LOCUS       DASUEG010000001         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1325421, whole genome shotgun sequence.
ACCESSION   DASUEG010000001 DASUEG010000000
VERSION     DASUEG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325421"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFX11_00005"
     CDS             <1..1194
                     /locus_tag="VFX11_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX4937025.1"
                     /translation="QPERDHIDHDYIDRIKTMVGHAKKHGLYTIIDMHQDAWGKHIAS
                     NGSEQCGFFETAIGWDGAPHWATLTNGASTCRLRVREISPAVATAFENFWRNREGIQD
                     ELIKVWGVLAREFADEPAVAGYDLLNEPNFGLSVGISQTLLMGNYYSKAIRAIRAAEK
                     GVPGGFAHIAFFEPSVEWSAFGVTLMPLGLFLFDNNIVFAPHLYGGSITVTGTIGSGY
                     QAAKKVADLYRTPFWSGEWGWFGSAEESEPQIWAYAQHEDAYRIGGAVWQWKQACGDP
                     HAQGIWSGREIEGEQNHVNVTWCPGDLPLRISPAYQRVLSRARPLYAPGELVALHSNP
                     YTGSMRLEGNAKASGTLLLWVPEREGVAGPEVRGGEVQGVVEVPGGYQVSVTVASRYA
                     VEVDY"
     gene            complement(1197..1526)
                     /locus_tag="VFX11_00010"
     CDS             complement(1197..1526)
                     /locus_tag="VFX11_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX4937026.1"
                     /translation="MTSEAPTKAVALTYDGQHAPVVSASGASELADEILRIAREHEVP
                     IYENPELVEILARLEVGDEIPELLYRAIAEIIAFVYMLKGKTPAGFGRPARDGVAALE
                     HRPNNDG"
     gene            complement(1523..3364)
                     /locus_tag="VFX11_00015"
     CDS             complement(1523..3364)
                     /locus_tag="VFX11_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX4937027.1"
                     /translation="MIEIPRGSAATPTATPSSTATPPAAAATSAATSAADVAADSVAK
                     SLEAVVVEVKARQASGNLEKPAWDILLKLNPAATASAGAVPVSSLTPEQLRALQNGQA
                     LLVRTQAPFPLPVDTRLNVNATAGQGIAIQQILPPGNSMPQQLVALLKQWVPAQQSAL
                     PLLANVLQLLSRSSAQTLATLPAPLQAVLQQLAQTLPNPDQIRQPGQMQAVVQHSGLL
                     LEAKLASLIAHLQKLPLDTNAPTGEPHAPNLQPNPSPAGTLAGLARAVRESFQRLVQD
                     SPDSSETSPATNAKSSATTTPVAPDLAEALGKDFKLALQKLEVALRDAQGQPAAEKNA
                     AQAKPGTAALAPAQADADTAAAGKNATPVAEPGAETPEQLLARLMNRHGRSGQPGIAG
                     TERAASHPGIQRYADAVRGETRLDTGKPTAADLLLLPPLPGQVLVQPHPRSRPTLKGD
                     EMADAIVSILLKQVRGALARMTLHQLTSSAQKQDNNAQPTLSFEVPFLHAGQVDMFQF
                     KIEQEPQTEQERRDKSLTKRWQVQLGFDIEGLGPMFCQLSLAGNNMSVTFWAAWENTL
                     AQTKAHFNFLEQALTDMGIHVEKMQGHLGIPENDRTGVRNQLVDIKT"
     gene            complement(3430..4185)
                     /locus_tag="VFX11_00020"
     CDS             complement(3430..4185)
                     /locus_tag="VFX11_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011218882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4937028.1"
                     /translation="MEIDVLHLCVLILAGVAAGFINTLAGGGSLFTLPALMLLGMPAD
                     IANGTNRIGVFLQSVAGVWGYRRHGKLEQAALVPILVPSVLGALIGALTASYIPPAIL
                     KPVLLGSMMAMMLLVLLKPSTFPAMGETTFTVAEKPAAWGWLFVAGLYGGFVQAGVGF
                     LLLTVLAGILRYDLVRANALKMACTVVFGLLPLAVFIVRDQVLWLPGLIVGISTIVGV
                     QLSVKFAVTAHQTTLKWIFLGMGMLVCVAALLK"
     gene            4339..4998
                     /gene="ccmA"
                     /locus_tag="VFX11_00025"
     CDS             4339..4998
                     /gene="ccmA"
                     /locus_tag="VFX11_00025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX4937029.1"
                     /translation="MSELLLHVKGLSCERDDRMLIRDLAFDLHGGEILQIEGPNGSGK
                     TTLIRILCGLSDDYEGELHWRGQPRHRNDDRFRQEHLYFGHLTGIKTSLSPRENLRWI
                     LQLKGVGTSSPALDTAIETALEKVGLASYEDVPVYALSAGQKRRVALARLQVEPALLW
                     VLDEPFTAIDRKGVAELEALIQQHAKRGGSVLLTTHHALDIPGLRKLQLGLGGGQWAL
                     N"
     gene            5040..>5705
                     /gene="ccmB"
                     /locus_tag="VFX11_00030"
     CDS             5040..>5705
                     /gene="ccmB"
                     /locus_tag="VFX11_00030"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX4937030.1"
                     /translation="MAQGFKAMLRRDLVVAFRQRSDLVNPLFFFIMVIALFPLGIGPE
                     PSTLQKIAPGVIWIAALLSTLLSVDSLFRQDFDDGSLELALLSPQPLFVLVLGKIVAH
                     WLVTALPLILVSPVLGVMLYMDADTIQATLCSLLLGTPVLSMISAIGAALTVGLRKGG
                     VLVAIIALPLYVPVLIFGTGAVEAASMGMAFGGQLAILGAFLILALAFAPFAIAAGLR
                     ISLN"
BASE COUNT         1017 a         1671 c         1838 g         1179 t
ORIGIN      
        1 cagcccgagc gcgatcatat tgaccatgac tacatcgacc gcatcaaaac gatggtgggg
       61 cacgcaaaaa agcacggctt atatacgatt atcgacatgc accaggatgc atggggtaaa
      121 cacattgcca gcaatggctc ggaacaatgc ggattttttg aaaccgctat cggttgggat
      181 ggcgcaccgc attgggctac gttgactaac ggtgcctcta cgtgtcgatt gagagtgcgc
      241 gaaatatccc ccgcagtcgc aacggctttc gagaatttct ggcgtaaccg tgaaggcata
      301 caggatgaat taatcaaggt gtggggcgtt ctggcgcgtg aatttgcaga tgaacccgcc
      361 gttgcaggat acgatttgct gaatgaaccg aatttcggtt tatcggtcgg aatttcccaa
      421 acgcttttga tgggtaatta ctacagcaaa gccattcgag cgatacgcgc ggctgaaaaa
      481 ggcgtacccg gtggttttgc acacatcgca ttttttgagc ccagtgtcga gtggtcggcc
      541 tttggcgtaa ccctgatgcc actgggcttg ttcctgttcg ataacaatat tgtcttcgcg
      601 cctcacttgt atggcggatc aatcaccgta acgggcacta tcggctccgg ctatcaggcg
      661 gccaaaaaag ttgccgatct gtatcgcact ccgttctggt cgggggaatg gggctggttt
      721 ggcagtgcag aagaaagtga accgcagata tgggcctatg cccaacacga agatgcctat
      781 cgcattggtg gtgcggtctg gcagtggaaa caagcctgtg gtgacccgca cgcgcaaggc
      841 atctggtctg ggcgcgagat cgaaggcgaa cagaatcacg tgaatgttac gtggtgcccg
      901 ggcgatcttc cgttacgtat atcaccggcg tatcaacgcg ttttgagccg ggcccggccc
      961 ttatatgcgc caggtgagct ggtggcgttg catagcaatc catatacagg atcgatgcgt
     1021 ctggagggca atgccaaagc cagcggcacg ctactgcttt gggtgccgga acgtgagggg
     1081 gttgcaggcc ctgaagtgag agggggcgag gtgcagggcg ttgtggaagt gcccggcggc
     1141 tatcaggtga gtgtgacggt ggcatcccgc tatgcggttg aagtggatta ctgagcttac
     1201 ccgtcattgt tggggcgatg ctccagcgcc gccaccccat cccgcgccgg cctcccgaac
     1261 cccgccggcg ttttcccctt caacatataa acaaacgcaa tgatctcggc gatggcgcga
     1321 tacaacaact ccggaatttc gtcgcccact tccagccggg ccagaatctc caccagttcg
     1381 ggattttcat agatcggcac ttcgtgttcc cgggcgatgc gtaaaatttc atccgccagt
     1441 tccgacgcgc ccgacgcact caccaccggc gcgtgctggc cgtcgtacgt caatgccact
     1501 gccttggtgg gcgcttcgct ggtcatgtct tgatgtccac cagttgattg cgcactccgg
     1561 tgcgatcgtt ctccggaata cccaggtgtc cctgcatttt ttccacatgg atgcccatgt
     1621 ccgtcagcgc ctgctccaga aaattgaagt gcgccttggt ctgcgccagt gtgttttccc
     1681 aggcggccca gaaggtcacg gacatgttgt tgcccgccag actcagctgg cagaacatgg
     1741 ggccgagccc ttcgatgtcg aaacccagtt gcacctgcca gcgtttggtg agcgacttgt
     1801 cgcggcgctc ctgttccgtt tgcggttcct gctcgatctt gaactggaac atgtccacct
     1861 gaccggcgtg cagaaacgga acttcaaaac tcagggtggg ttgcgcgttg ttgtcctgtt
     1921 tctgggcgct gctggtcagc tggtgcaacg tcatgcgggc cagtgcgcca cgcacctgct
     1981 tcagcagaat gctgacgatg gcgtctgcca tttcgtcgcc tttcagggtg gggcggctgc
     2041 ggggatgggg ttgcaccagc acctggccgg gcagtggcgg cagcagcaga aggtcggcgg
     2101 cggtgggctt gccggtgtcc agacgggttt cgccgcgcac ggcgtcggca tagcgctgga
     2161 tgccgggatg agaggccgcg cgctcggtgc ccgcaatgcc gggctggccg ctgcgaccat
     2221 gacggttcat gagccgggcc agcaactgct ccggtgtttc ggccccgggc tcggcgacgg
     2281 gcgttgcatt ttttcccgca gcagctgtat ccgcgtccgc ctgcgcgggt gccagtgctg
     2341 cggttcccgg cttcgcctgt gcggcatttt tttccgctgc cggttgcccc tgcgcatccc
     2401 gcaacgcgac ttccagtttc tgcagtgcca gtttgaaatc cttgccgaga gcttccgcca
     2461 gatcgggcgc cacgggcgtt gtcgttgcag acgatttggc attggtggcg ggcgaggtct
     2521 cgctactgtc gggggaatcc tgcaccagcc gctggaaaga ttcgcgcacg gcgcgcgcca
     2581 gtccggccag tgtaccggcg ggcgacggat ttggttgcag gttgggcgcg tgcggctcgc
     2641 cggtcggggc gttggtatcc agtggcagtt tttgcaggtg cgcaatcagg ctggccagtt
     2701 tggcttccag caacagtccg ctgtgctgca ccaccgcctg catctggccg ggctggcgga
     2761 tctgatcggg attgggcagt gtctgcgcca gttgctgcaa tacggcctgc agcggagcgg
     2821 gcagtgtggc gagggtttgc gccgatgacc tactcaacag ttgcagcaca ttggccagca
     2881 gcggcagcgc gctttgctgc gccggcaccc actgcttcag cagcgcgacc agttgctggg
     2941 gcatgctgtt gccgggcggt agaatctgct ggatcgcgat gccctggccc gccgttgcgt
     3001 tgacattgag ccgcgtgtcc accggcagcg gaaacggggc ctgggtacgc accagcaggg
     3061 cttgcccgtt ttgcagtgca cgcagctgtt ccggcgtgag gctgctgacg gggacagcac
     3121 cggcacttgc agtggcagcc ggattcagct tcagcagaat atcccaggcg ggcttttcca
     3181 gattgccgct ggcctgccgc gctttcactt ccaccaccac ggcttccagc gatttggcca
     3241 ccgagtcggc agccacgtcc gccgccgacg ttgctgccga tgttgcagcg gcggcgggcg
     3301 gtgtcgcagt gctggacggg gtggcagtgg gcgttgccgc cgaaccgcgt ggaatctcga
     3361 tcatatggcc tcgtttttcg ttcagtatag gccaggtcgc gagggttcag agcgcagctg
     3421 tcaacgctgc tatttcagca gggcggccac acacaccagc atgcccatgc ccaggaaaat
     3481 ccacttcaag gtggtctggt gggcggtcac ggcgaacttc acgctcagct gcacgccgac
     3541 aatggtgctg atgcccacga tcagccccgg cagccacagc acctgatcgc gcacgatgaa
     3601 caccgccagc ggcagcaggc cgaacaccac cgtgcaggcc attttcagcg cgttggcccg
     3661 caccaggtca taacgcagga tgcccgccag caccgtcaac agcagaaaac ccacgccggc
     3721 ctgcacgaat ccgccgtaca gtcccgccac gaacagccag ccccaggcgg cgggcttttc
     3781 cgcgacggtg aaggtggttt cccccattgc gggaaaggtg gacggcttca gcaggaccag
     3841 cagcatcatg gccatcatgc tgcccagcag gacgggtttg agaatcgcgg ggggaatgta
     3901 ggaggctgtc agcgcgccga tcagtgcgcc cagcacgctg ggcaccagga tcggcaccag
     3961 ggctgcctgt tccagcttgc catggcggcg atagccccac acaccggcta cggattgcag
     4021 aaatacaccg atgcgattgg tgccgttggc gatgtcggcg ggcatgccca gcagcatcag
     4081 ggcgggcagg gtgaacagcg agccgccacc cgccagggta ttgatgaagc cggcggctac
     4141 acccgcaagg atcagcacgc acaggtgcag aacgtcgatt tccatggtcc atccaccgcc
     4201 cactgcaagg agcaggcagt gtagtgagtg gcccgttgcg ctgcaattcc cgacagccac
     4261 tggccacagg caggggacat tgcggtgcga ttcgatataa tgtccgcctt tcaaccaaat
     4321 gctgttaggc catagccggt gtcggaattg ttgctgcatg tgaaaggact gtcctgcgaa
     4381 cgggatgacc ggatgctgat ccgcgatctg gcgttcgacc tgcatggcgg cgagatcctg
     4441 cagatcgaag gccccaatgg cagcggcaaa accacgttga ttcgcatcct gtgcggcctg
     4501 tccgatgatt acgaaggcga gctgcactgg cgcggccaac cccggcatcg caatgacgac
     4561 cgcttccgcc aggaacatct gtatttcggc catctcaccg gcatcaagac atcgctttcc
     4621 ccccgtgaaa acctgcgctg gatactgcaa ctgaaaggtg tgggcaccag cagccctgct
     4681 ctggatacgg ccatcgaaac cgcgctggaa aaagtggggc tggccagtta cgaggatgtg
     4741 ccggtgtatg cgctgtcggc cgggcagaag cggcgggtgg cgctggcgcg gctgcaggtg
     4801 gagccggcgc tgttgtgggt gctggacgaa cctttcactg ccattgaccg caagggcgtg
     4861 gcggagctgg aagcgctgat ccagcaacat gcaaagcgtg gcggcagtgt gctgctgacg
     4921 acccatcacg cactggacat tcctggcctg cgcaaattgc agctgggact ggggggtggc
     4981 cagtgggcac tgaactgaac ccggcttctg tgaacccggc tgttgtgaaa gcaccttcca
     5041 tggcgcaggg cttcaaggcc atgctgcggc gcgatctggt tgtcgcgttt cgccagcgga
     5101 gcgatctggt caacccgttg tttttcttca tcatggtgat cgccctgttt ccgctgggca
     5161 ttggccccga gccgtccacg ttgcaaaaaa tcgcgccggg ggtgatctgg atcgccgcgc
     5221 tgctgtctac cttgttatca gtggacagcc tgttccgcca ggatttcgac gacggttcgc
     5281 tggaactggc gctgctcagc ccgcagcccc tgttcgtgct ggtgctgggc aagatcgtcg
     5341 cccactggct ggtgacggcg ctgccgctga ttctggtgtc gccggtgctg ggtgtcatgc
     5401 tgtatatgga tgccgacacc attcaggcga cgctgtgcag cctgttgctg ggcacgccgg
     5461 tgctgagcat gatcagtgcg atcggcgccg cgctcacggt tggcttgcgc aagggcggcg
     5521 tgttggtggc catcatcgcg ctgccgctgt atgtgccggt gctgattttt ggcaccggcg
     5581 cggtggaggc agcgtcgatg ggcatggcgt tcggcgggca actggcgatc ctgggcgctt
     5641 ttctgatatt ggcgctggcg ttcgcaccgt tcgctatcgc cgcgggtctg cgcatcagtt
     5701 tgaac
//
LOCUS       DASUEG010000002         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1391732, whole genome shotgun sequence.
ACCESSION   DASUEG010000002 DASUEG010000000
VERSION     DASUEG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391732"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3194)
                     /locus_tag="VFX11_00035"
     CDS             complement(<1..>3194)
                     /locus_tag="VFX11_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937031.1"
                     /translation="GFTLELRYDVTRFDDLQLLQRIVHINQQILFGNLDKVSQLQFLS
                     VPEYNRLQKWSGENRATDTETVVGKFERQAAATPDAVALISGDVQLSYRELNAQSNRL
                     AHWLIGKGVKANVRVGICLNRSIDLVVSVLAVLKAGGAYVPMDPAYPRERLAFILQDS
                     AAPLVLARSEFSAALDVVKSEPVLLDRLSLADMPAHNPGVAIDPDQQIYVIYTSGSTG
                     QPKGAMVQHRGETNLQHWYLAALNVTAQDRTLLVSAVGFDLTQKNLFAPLLAGAALVL
                     PAMELFDEAELLHLIEQHRVTWVNCAPSAFYPLVESAAPHGYRALQSLRYVVLGGEPI
                     RLGALYPWLSTHGCHAQLINSYGPTECTDVVAWHKVDHIESVNQVLPIGKPVDNLQLF
                     ILNDNLQQVVPGLSGEICVAGVGVGLGYINRDELTQSVFIQNPFGAGKLYRTGDLGRY
                     LPNGDIEYLGRKDFQIKLRGLRIELGEIEHALKQLPDVEDGLALVKDDKLIAYVVSPQ
                     PVVEDWRGRMREHLPEYMIPAAIVTLPQWPLTPNGKIDRKALPDPAAVIAQASVYVAP
                     RSELEAQLAAIWQDLLQKPQVGMLDNLFDLGGNSLLATRILSRVKKQLGVQIAVRELF
                     IAPTIADLAVAINRAQQIENVPPITTVDYTVPQPLSFAQQRLWFLDQLDPGNTAYNMP
                     GAFRVKGRLDVSAFEQALQEIVRRHAVLRSHTVMVDEQPCQLIGDGGEWHLQKRDYST
                     LSDDALEPKIRERIAQWQAHAFDLATGPLFQVELVSLRPDDWLLLVNTHHIASDGWSN
                     GVMMRELGILYDAFLHRRPSPLPPLRIQYVDFAQWQRQWLSGDELERQVGYWRQQLTG
                     VEVLNLPTDRPRRADTSFAGNVLNFSIDPQLTAQLNQLTRQQGATLYMTLMAAYMVLL
                     AKYSNQTDISVGSPIAGRNHEDIESLIGFFVNTLVLRGDVQSSMPFVELVQQLRQNTL
                     NAYAHQDVPFERLVDEIVRERDLLHSPLFQVMFSLQNVPMDANFTLPGLKIANLPQQD
                     VVAKFDLDFSMVELDGGLKGEVVYRTGLFEKRTV"
BASE COUNT          654 a         1035 c          905 g          600 t
ORIGIN      
        1 cacggtgcgc ttctcgaaca ggccggtgcg gtacaccacc tcgcctttga ggccgccatc
       61 cagttccacc atgctgaaat ccagatcgaa cttcgccacc acatcctgct gcggcaggtt
      121 ggcgattttc aggcccggca aggtgaaatt ggcgtccatc ggcacgttct gcaacgagaa
      181 catcacctgg aacagaggcg aatgcagcag atcgcgctcg cgcacgattt catccaccag
      241 gcgctcgaac ggcacgtcct ggtgagcgta ggcattcagc gtgttctggc gcagctgctg
      301 caccagttcc acgaacggca tcgacgactg cacatcgccg cgcaacacca gcgtattcac
      361 gaaaaagccg atcagcgatt caatgtcttc gtgattgcgc ccggcaatcg ggctgcccac
      421 gctgatatcg gtctggttgc tgtatttcgc cagcagcacc atgtaggccg ccatcagcgt
      481 catgtacagg gtggcgccct gctgccgggt cagctggttg agctgcgcag tcagctgcgg
      541 gtcgatggaa aaattcagca cattgccggc aaagctggtg tcggcgcggc gcgggcgatc
      601 ggtcggcaga ttcagcactt ccacgccggt cagctgctgg cgccagtaac cgacctgacg
      661 ttccagctca tcgccgctca gccactgccg ctgccactgg gcaaaatcca cgtactgaat
      721 ccgcagcggt ggcagcggcg aaggccgcct gtgcaggaaa gcgtcgtaca gaatgcccag
      781 ttcgcgcatc atcacgccgt tggaccagcc atcggacgca atgtgatggg tattcaccag
      841 caacagccag tcatccggcc gcagtgaaac cagttccacc tggaacagcg ggcccgtcgc
      901 cagatcgaac gcatgcgcct gccactgggc aatacgctcg cgtattttcg gctccagcgc
      961 gtcgtcagac agggtggaat aatcgcgttt ctgcagatgc cattcaccac catcgccaat
     1021 cagctggcag ggctgttcat ccaccatgac ggtgtgcgac cgcagcacgg catgacgccg
     1081 cacgatttcc tgcaacgcct gctcaaacgc gctcacatcc agccggccct tgacgcggaa
     1141 cgcaccgggc atgttgtagg ccgtgttacc cggatccagc tggtccagga accacaaccg
     1201 ctgctgtgcg aacgacagcg gctgcggcac ggtgtaatcc acggttgtaa tcggcggaac
     1261 attttcgatc tgctgcgcgc ggttgatcgc caccgccaga tcggcaatgg tgggcgcaat
     1321 gaacagttca cgaaccgcga tctgcacgcc cagctgcttt ttcacgcgcg acagaatgcg
     1381 ggtggccagc agcgagttgc cgccgagatc gaacaggttg tccagcatcc ccacctgcgg
     1441 cttttgcagc agatcctgcc agatcgcggc cagctgcgct tccagttcgg agcgcggcgc
     1501 cacatagacg ctggcctgcg caataacggc ggccggatcc ggcaatgcct tgcgatcgat
     1561 tttgccgttc ggcgtcagtg gccactgcgg cagcgtcaca atggccgcgg gaatcatgta
     1621 ttccggcaaa tgctcccgca tccgcccgcg ccagtcctca accaccggct gcggcgaaac
     1681 cacatacgcg atcagcttgt cgtccttcac caacgccagg ccatcttcca cgtccggcaa
     1741 ctgtttcagc gcgtgctcga tttcacccag ttcaatgcgc aggccgcgca gtttgatctg
     1801 gaaatccttc cgccccaggt attcgatatc gccattgggc aggtagcggc ccaaatcgcc
     1861 ggtgcgatac agcttgccgg cgccgaacgg attctggata aacacactct gcgtcagttc
     1921 atcgcgattg atatagccga ggccaacccc cacaccggcc acacaaatct cgcctgacag
     1981 acccggcacc acctgttgca ggttgtcgtt gagaatgaac agttgcaggt tgtccaccgg
     2041 cttgccgatg ggcagcacct ggttaaccga ctcgatgtga tccaccttgt gccaggccac
     2101 cacgtcggta cattcggtgg ggccgtaact gttgatcagc tgtgcgtggc aaccgtgagt
     2161 cgacagccag ggatacaacg cccccagacg gatcggttca ccacccagca cgacatagcg
     2221 caggctctgc aatgcgcggt aaccatgggg ggcggcagat tccaccagcg gataaaacgc
     2281 gctgggcgcg cagttcaccc aggtcaccct gtgctgttcg atcagatgca gcagttcggc
     2341 ttcatcgaat agttccatcg ccggcagcac aagtgcggcg cctgccagca ggggcgcgaa
     2401 cagatttttc tgggtcaggt cgaagcccac cgcactcacc agcagggtgc ggtcctgcgc
     2461 agtcacattc agcgccgcca ggtaccagtg ctgcaggttg gtttcgccgc gatgctgcac
     2521 catggcgcct ttcggctgcc cggtggagcc ggaggtataa ataacgtaaa tctgctgatc
     2581 gggatcgatc gccacacccg ggttgtgcgc cggcatgtcc gccagcgaca gacgatccag
     2641 cagcaccggt tctgatttca ccacgtccag cgcagccgaa aattcactgc gcgccagcac
     2701 cagtggcgca gcgctgtcct gcaaaatgaa cgcaaggcgc tcgcggggat aggccgggtc
     2761 catcggcaca taggcgccac cggctttcaa caccgccaat accgacacca ccagatcgat
     2821 ggaacggttc aggcagatgc caacgcgcac attcgctttt acacccttgc caatcagcca
     2881 gtgcgccagc cggttgctct gtgcattcaa ctcacggtaa ctcagttgca catcaccact
     2941 gatcagcgcc accgcatccg gcgtagccgc cgcctgacgt tcgaatttgc cgacaaccgt
     3001 ttccgtatcg gtggcccggt tttcaccact ccacttctgc aggcggttgt attcaggtac
     3061 cgacaggaac tgcagctgcg acaccttgtc cagattgccg aacaggatct gctggttgat
     3121 atgcacgata cgctgcagca attgcaggtc gtcgaaacgg gtgacgtcgt aacgcagctc
     3181 cagggtaaag ccgc
//
LOCUS       DASUEG010000003         4961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_419711, whole genome shotgun sequence.
ACCESSION   DASUEG010000003 DASUEG010000000
VERSION     DASUEG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419711"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFX11_00040"
     CDS             complement(<1..383)
                     /locus_tag="VFX11_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937032.1"
                     /translation="MHSILNPFRRRAWPCLLGALGILASGDALAGIAQWSSTNFQLLY
                     GDNYQSIFFNEATGKLDSVDDVRSVITVEHVNGWKYGDNFMFFDITNADRTTETTTNY
                     YGEISPRLSFGKMTGVNMNHGLLNDV"
     gene            749..1426
                     /locus_tag="VFX11_00045"
     CDS             749..1426
                     /locus_tag="VFX11_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4937033.1"
                     /translation="MTAQHIYQSLRSMILDLELAPGSRFTETQVADYFQVSRTPVREA
                     LQRLEVEKQITIRPKQGCFVRVVDIARIRDYYDVRVAIETLAVRLACVHMPDAALREL
                     ATQWDPANLFFGAESSDRIKQAEEAFHIALAQGSGNEALVRYLTDINDHIRVVRRLGW
                     PDYSSIEDTYAEHHDICRLMLNRDAEAAEACMLQHIRKSQTRADQITLAQLSEPARNP
                     FASAALQ"
     gene            1539..2591
                     /locus_tag="VFX11_00050"
     CDS             1539..2591
                     /locus_tag="VFX11_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937034.1"
                     /translation="MIAVNRPAHPYSDAQLNRLRLSADPLTDAVALRIDQMPADSLLK
                     EVIFLAKSEIGIYQEWLDHLHTVPAWIDHAQLERARQLQLSFANERRLAWAINGLLRT
                     RTRFRCHLINPGQRLNEFLQLQNCLYQPATLLPGGPGHSQLMEQRLNRAMERWALRGH
                     GRNLLTLGEPFNQETLALEMLETGLLTVQGMAALGVTLQPDDQLALHHFNRYVAWLSG
                     VDGRLLAETPDQALQLLQRLQQRYGCAPRAEQAELLQMLAEQRTLPLPASLLPAAACH
                     CLGDARVDPTAPLPQRALHLYCTANRGKTFAFYHVPGVAPLTARLNLRKPLPKRNPPA
                     LQALPAPRNSSARHNR"
     gene            complement(2593..4542)
                     /locus_tag="VFX11_00055"
     CDS             complement(2593..4542)
                     /locus_tag="VFX11_00055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937035.1"
                     /translation="MRAILRFLLPGVLLLSLSSAALASRVLVQGGDLMLTDPAVGSQV
                     PPAAVRYRLDEQAFDSAPLQAPSPDQLLAPEQEDLWQVLPRKGLNFGMLNQPVWLRFT
                     VRNPSAQSGRWLLVMPWSQLNHIEFNVRHGDGHWGERFEAGYDVHYSRRYGQHRLFQF
                     PLQLAPGEQATVLIRVQAKYITFAPMLVWTPEAFQQQDFNDNMAYGIAFGILLAMLLY
                     NLSLSFVLRSSSYFFYSAYVFSIILYQLAVSGYGGRYLWADSDFMQVRAFSLFAAFSF
                     FCAGLYVRNFLRLKKRGGWLLHLNTWITGYWLVSTLVTVFYTPLLLRRLGDPMALVTC
                     VAALTTTIHLWRRGDVSARYFTIAWGQLILATMGVVLMFEGVLPYDSLTEKLQLVGFV
                     LETLLLSFALAERINRERHQRELLQQTALDMTRQASEERRAKLEAQAEALALQQRHTD
                     ELELRVLDRTAELERTMRNLELANRELAKLSVTDPLTGLHNRRYFDEVLASEIQRGQR
                     QQSPVSLILVDVDHFKRINDTYGHLIGDECLKLVAATLRQVVVRGTDLVARYGGEEFA
                     VILPATSDEDARLVAERIRIAIEKTQFIHAGRRISLNASLGIAGRIPLQHENSARLIA
                     AADEALYRAKETGRNRVVVADLAQR"
     gene            4850..>4961
                     /locus_tag="VFX11_00060"
     CDS             4850..>4961
                     /locus_tag="VFX11_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HEX4937036.1"
                     /translation="MYQATVPVFIRMLGNLGRIIDKAQGFAETKRIDTTVL"
BASE COUNT         1074 a         1583 c         1428 g          876 t
ORIGIN      
        1 acgtcgttga gcaggccgtg attcatgttc acgcccgtca tcttgccgaa actcaggcgc
       61 ggtgaaattt cgccgtaata attggtggtg gtttcggtgg ttcggtcggc gttggtgatg
      121 tcgaagaaca tgaagttgtc gccatacttc cagccgttca cgtgttccac cgtgatcacg
      181 ctgcgcacgt cgtcgaccga atccagcttg ccggtggcct cgttgaaaaa aatcgactgg
      241 tagttgtcgc cgtacagcag ctggaaattg gtggaactcc actgcgcgat tccggccagg
      301 gcatcccccg acgccagaat gcccagggca cccagcagac agggccaggc ccggcggcga
      361 aaaggattta aaatggaatg caaacggctg tgcccggttt tcatcatgtg ctcccccgaa
      421 tcattggttg gtatgtgcaa ttctgctcga accaggtggc atgcccccgc atttccgcct
      481 gttgtataca acgttgcatt gtctatgcca gactgtccgg agtggcacca ggctcagaaa
      541 aaaggctttt caatcagcaa gatatcgccc tgcattgggc cgcgagtgca cgcaccgccg
      601 gccgccggtg ataaaaaccg gtgccctccg acaccagctg ctcttgaaac gggcagccat
      661 cggcgacaga ttagggcaag ggaaatgcat ggtttccggg acggcgcccc tcaggcgcag
      721 cattagggag aacgaagtgc gatcaaaaat gaccgcccag cacatatacc agtccctgcg
      781 cagcatgatc ctggatctgg aactggcgcc gggcagccgc ttcaccgaaa cccaggtggc
      841 cgactatttt caggtcagcc gcacgccggt gcgggaggcg ctgcagcgtc tggaagtgga
      901 aaagcagatc accatccgtc ccaagcaggg ctgtttcgtg cgggtggtgg atatcgcccg
      961 catccgcgat tactacgacg tgcgggtggc gatcgaaacc ctggcggtac ggctggcctg
     1021 cgtgcacatg cctgacgccg ccctgcggga actggccacc cagtgggacc cggccaacct
     1081 gtttttcggc gccgaatcca gcgatcgcat caagcaggcg gaggaagctt tccacatcgc
     1141 cctggcccag ggcagcggca acgaggcgct ggtgcgctac ctcaccgaca tcaacgatca
     1201 catccgcgtg gtacggcggt tgggctggcc tgactacagc agcatcgagg acacctacgc
     1261 cgaacaccac gacatctgcc gcctgatgct gaaccgggat gccgaagcgg cagaagcctg
     1321 catgctgcag cacatccgca aaagccagac ccgcgccgac cagatcaccc tggcccagct
     1381 gagcgagccg gcccgcaatc cgttcgcgag cgctgcgctg caatgagtgc taaccgaatt
     1441 agggcaattt gcagccaatg gcttgcgccc cccaaggaat tgcgattaat gtctctatca
     1501 agccccacaa acagaataaa aaccgcggga cctattccat gatcgccgtc aaccgcccgg
     1561 cccaccccta ttccgacgcg caactcaacc gtctgcgcct gagcgccgat ccgctgacgg
     1621 acgccgttgc cctgcgcatc gaccagatgc cggccgacag cctgctgaaa gaagtcatct
     1681 tcctggccaa gagtgaaatc gggatttatc aggaatggct ggaccatctg cacaccgtgc
     1741 cggcctggat cgatcacgca caactggagc gcgcccgtca gctgcaactg agctttgcca
     1801 acgaacgccg gctggcgtgg gcgatcaacg gcctgctgcg cacccgcacc cgctttcgct
     1861 gccacctgat caatcccgga caacggctga acgaattcct gcagctgcaa aactgcctgt
     1921 atcagcccgc caccctgctg ccgggcggac ctggccacag ccagttgatg gaacaacggc
     1981 tgaaccgcgc catggaacgc tgggccctgc gcggacacgg ccgcaacctg ctgacgctgg
     2041 gcgaaccgtt caaccaggaa acgctggcgc tggaaatgct ggagaccggc ttgctgacgg
     2101 tgcaaggcat ggcggcactg ggtgtgacac tgcaacctga cgaccaactc gcactgcatc
     2161 atttcaaccg ttacgtcgcc tggctgtccg gtgtggatgg acgtctgctg gcggaaacgc
     2221 cggaccaggc cctgcaactg ctgcagcgat tgcagcaacg ctatgggtgt gcacccagag
     2281 cagagcaggc agagctgctg cagatgctgg cggagcagcg cacgttgccg ctgccggcca
     2341 gcctgctgcc ggccgctgcc tgtcactgtc tgggcgatgc acgggtggac ccgactgcgc
     2401 ccctcccgca acgggcgctg cacctgtact gcaccgccaa ccggggcaag acgttcgcgt
     2461 tctatcacgt gcccggtgtc gccccgctca ccgcgcggct caacctgcgc aagccattgc
     2521 ctaagcgcaa tccacccgca ttgcaggcat tacccgcacc ccgtaacagc agcgcgcgcc
     2581 ataaccgctg agctagcgct gcgcgagatc cgccaccacc acccggttgc gtccggtttc
     2641 cttcgcccgg tacaacgcct catcggccgc cgcgatcagg cgggcactgt tttcatgctg
     2701 cagcggaatc cggccggcga tgcccaggct ggcattcagt gaaatccttc ttcccgcgtg
     2761 aatgaactgt gttttttcga tggcgatgcg aatgcgttcc gccaccagac gcgcatcctc
     2821 atccgaggtt gccggcagga tcaccgcaaa ttcctcaccg ccgtaacggg ccaccagatc
     2881 ggtgccccgc accaccacct gacgcagggt ggccgccacc agcttcaggc actcgtcgcc
     2941 gatcagatgg ccataggtat cgttgatgcg cttgaaatgg tccacgtcca ccaggatcag
     3001 cgataccggt gactgttgac gctggccacg ctggatttcg ctggccagca cctcatcgaa
     3061 ataacgtcgg ttgtgcaggc cggtgagagg atcggtcacg ctcagcttgg ccagctcgcg
     3121 gttggccagt tccagattgc gcatggtgcg ctccagctcg gcggtgcgat ccagcacgcg
     3181 caattccagc tcatcggtgt ggcgctgctg caaggccagc gcttcggcct gcgcttccag
     3241 tttggcacgt cgttcctcgc tggcctgacg ggtcatgtcc agcgccgtct gctgcagcag
     3301 ttcgcgctgg tgacgctcac ggttgatgcg ttccgccagc gcaaaggaca acagcagggt
     3361 ttccagcacg aaaccgacca gctgcaattt ttccgtcaga ctgtcgtagg gcaacacgcc
     3421 ttcaaacatc agcaccacac ccatggtggc caggatcagc tggccccagg cgatggtgaa
     3481 atagcgcgcc gacacgtcac cccggcgcca caggtgaatg gtggtggtga gcgcggcgac
     3541 acaggtgacc agtgccatcg gatcgcccag cctgcgcagc agcaacggcg tgtagaacac
     3601 cgtcaccagc gtagacacca gccagtaacc ggtgatccag gtattcaggt gcagcagcca
     3661 gccaccgcgt tttttcagcc gcagaaaatt gcgcacatac aggccggcgc agaaaaaact
     3721 gaatgcggca aacagcgaaa acgccctgac ctgcataaag tcagagtcgg cccagagata
     3781 gcggccacca tacccgctca ccgccagctg atacagaatg atggaaaaca cataggccga
     3841 atagaaaaaa tagctgctgg aacgcagcac gaatgacagc gaaagattgt aaagcagcat
     3901 cgccagcaga atgccaaaag caatgccgta cgccatgttg tcgttgaaat cctgctgctg
     3961 gaacgcctcc ggcgtccaca ccagcatcgg cgcgaacgta atgtattttg cctgcacccg
     4021 gatcagcact gtggcctgct cgcccggcgc cagctgcagc ggaaactgga acagccgatg
     4081 ctgaccgtaa cgacgggaat aatgcacgtc gtaaccggct tcgaaccgct cgccccagtg
     4141 gccatcgcca tgccgcacat tgaactcgat atggttgagc tgggaccagg gcatcaccag
     4201 cagccagcgg cccgactggg ccgacggatt acgcacagtg aaccgcagcc atacgggttg
     4261 attcagcatg ccgaaattaa ggccctttct gggcagcacc tgccagaggt cctcctgctc
     4321 cggcgccagt agctggtcgg ggctgggcgc ctgcagtgga gcggaatcga aggcctgctc
     4381 atccagccgg taacggacag ccgcaggggg aacctggctg ccaacggcag gatcggtcag
     4441 catgagatcg ccaccctgca ccaacaccct ggatgccagg gctgcactcg acagcgacag
     4501 caacagcacg cccggcagca ggaaccgcag gatcgcgcgc aatggttgac aagaaaaaga
     4561 ggggaagatc atgcatagaa ataatagctt atggtccaac ggatagcgct gatatagcca
     4621 actattctga tacccccgga aaaagcctgg cgcgcggctg cgatgacgaa cgctaaggcg
     4681 cggcgttttt gacactccca aatccggtta gccctctgtt aagctgcgaa tgtcagtatt
     4741 ctgttgcacc acaccgggta tcccgttctt tggatttatc attcaggcac ctttttcagg
     4801 cctcaaactt caggcaccaa ccttcaggag agacacaatg caaatttcca tgtaccaggc
     4861 caccgtgccg gtattcatcc gcatgctggg caatctgggt cgcatcatcg acaaggccca
     4921 gggattcgcc gaaaccaagc gcatcgatac cactgtgctg g
//
LOCUS       DASUEG010000004         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1391609, whole genome shotgun sequence.
ACCESSION   DASUEG010000004 DASUEG010000000
VERSION     DASUEG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391609"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1120
                     /locus_tag="VFX11_00065"
     CDS             98..1120
                     /locus_tag="VFX11_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937037.1"
                     /translation="MSMKDFSTMIGHLRRRFFGASPKAPPVNLRGKKIMVTGASPGSI
                     GFETALTLAGWGACVTITTRSNANATADQLRRRLHQQGKSADIDSHVLDLADAGSVTH
                     FAQWFQKKHGSQLDVLINNAGIHLDLAGHWKQPQLTPDGFEIHWRTNYLGTQHLTLLL
                     LPLLKQAGKSTGNARIINVASHLHTKGTNAGLFAPIQPFHSWTAYGQSKLALIHAAFE
                     IQRRYASQGVQGFALHPGSIHTNISDKGLATRPLLRTLRNWFAPLEALVLLTPEQGAQ
                     TQILCATAAGLNGGIYYERCAPADCSEELDDLAAARRLWQETEVWVQNLQSEQGAGTR
                     KDVIHV"
     gene            1113..1556
                     /locus_tag="VFX11_00070"
     CDS             1113..1556
                     /locus_tag="VFX11_00070"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4937038.1"
                     /translation="MFEQTIYSRVVEINAPRERVWNILTDLKQYPQWNPFTYRVVTTL
                     AIGDPVDLYVRFRKRGEVTQKEQVCIVQKPERLAWGMKMGARFILCAQRDQVLTALDD
                     GHCTYQTWDAFSGLLTPLVMALFRQDIEDGFNAMAAALKVRAENT"
     gene            complement(1606..>3179)
                     /locus_tag="VFX11_00075"
     CDS             complement(1606..>3179)
                     /locus_tag="VFX11_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937039.1"
                     /translation="VYRDSGDNYLFMVESKFDPSIQPGVALSRSRFEDNAFTHGDNLK
                     QGVRTDYGTVSVRERTELELRFTDPATRDLHPGVRETIRNRHHVFVCYPGYPDYRHVP
                     VIGAGLTFKMPGSMDVWGLLCEGDLEEVYRERSIDLTLLTRLALLGGAGFAVAEYCKH
                     LLNVTGGATLLVNAGTGALILLVFWLLGLRPLRQRLQRLENFFLDIAEAGSSLQTRID
                     VDTFPKDETGQLALWINSFVDKLDDTVKQVLATATALTSASSTLQASSEIVSRSALEQ
                     HEIAQAAQHIMENITTTILDVADHSTETEKASREAHEISEKGGAVVTHAANGMAGVAQ
                     SVRDSTDIIRTLSQCADEIDGISNTIKEIAEQTNLLALNAAIEAARAGEQGRGFAVVA
                     DEVRNLSRRTTQATHEIARTICTIQTETGKAVRSMEMCNDLAQQGVLRADEASNALKH
                     IHTGALSTLERVQTVTQAVFAQKSLANQVNDQVQAMTVNASSSHQAVRDTLTTVNTLG
                     QLVLALQSAANKFRS"
BASE COUNT          685 a          981 c          861 g          652 t
ORIGIN      
        1 tgggaaaaga gcgcagtgct gctgcaacct tataccgaac cagcgcgaac cgcggaaccc
       61 gcatgattcg ctggtgtctg ccgtgcggag acaaaccatg tcaatgaagg acttttcgac
      121 catgatcggt cacctgcgcc gccgcttctt tggcgccagt cccaaggctc cacccgtgaa
      181 tctgcgtgga aaaaaaatca tggtcacggg agcttcgccc ggttccattg gttttgaaac
      241 ggcgctgaca ctggcaggct ggggcgcctg cgtgactatc accacccgca gcaacgccaa
      301 cgctactgct gaccagttgc gccgccgctt gcatcagcag ggcaagagcg ccgacatcga
      361 ttcgcatgta ctcgatctgg ctgatgcagg ttcagtaaca cactttgcac agtggtttca
      421 gaaaaagcac ggctcccaac tggatgtact gatcaacaac gccggcatcc atctggatct
      481 ggcgggccac tggaaacagc cgcaattgac acctgacggg ttcgagattc actggcgcac
      541 caattatctc ggcacccagc atctcaccct gctcttactg ccactgctga aacaggcagg
      601 caaatccacc ggcaatgccc gcattatcaa tgtcgcatcg catttgcata ccaaaggcac
      661 caatgccgga ctgttcgcac cgatccagcc attccattcc tggacggcgt atggccaatc
      721 caaactggca ctgattcatg cggcatttga aatccagcgt cgctatgcca gccagggcgt
      781 acagggattc gccctgcacc ccggttccat tcataccaac atttccgaca agggacttgc
      841 cacccgtccg ctgctgcgca cattgcggaa ctggttcgct ccactggagg cactggtgtt
      901 gctgacgccg gaacagggtg cgcaaacgca gattctctgc gccaccgctg ccgggctcaa
      961 cggtggtatc tactacgaac gttgcgcgcc tgctgactgc agcgaagaac tggacgatct
     1021 ggctgctgca aggcgcctgt ggcaggaaac cgaagtgtgg gtgcaaaacc tccagtcgga
     1081 acagggtgcc ggcacacgca aggacgtcat acatgtttga acagacgatt tattcccgtg
     1141 tggtggaaat caatgcaccc cgtgaacggg tttggaacat tctgaccgat ctgaaacagt
     1201 atccacagtg gaatcccttc acctatcgcg tggtaacaac gctggccatt ggtgacccgg
     1261 tggatctgta cgtgcgattc aggaagcgcg gggaagtaac gcaaaaagaa caggtctgta
     1321 tagtgcaaaa accggagcgg ctggcgtggg gaatgaaaat gggggcacga ttcatactat
     1381 gcgcgcaacg ggatcaggta ctgacggcgc tggatgatgg acactgcacc taccaaacct
     1441 gggatgcctt ttccggtctg ctgacaccgc tggtgatggc actgttcagg caggacattg
     1501 aggacggctt caatgcaatg gcggctgcgc tgaaagtgcg ggcggaaaac acctagcccg
     1561 aatatttcac ctggattcgg tcaagcacca gccaccctca tcgagtcagc ttctgaactt
     1621 gttggccgcc gattgcagcg ccagcaccag ctgccccagc gtattcacgg tcgtgagcgt
     1681 atcacgcacc gcctgatgac tgctggacgc gttcaccgtc attgcctgta cctgatcgtt
     1741 cacctgattc gccagcgact tctgtgcaaa caccgcctgc gtcaccgtct gcacccgctc
     1801 cagcgttgac agcgcgccgg tatggatgtg tttcagcgca ttgctggcct cgtccgcccg
     1861 caacacgccc tgctgcgcca gatcgttgca catctccatc gaccgcaccg ccttgccggt
     1921 ttccgtctgg atggtgcata tggtgcgcgc aatttcgtgc gtagcctggg tggtgcggcg
     1981 cgacagatta cgcacttcat ccgcaactac cgcaaatccc ctgccctgtt caccggcacg
     2041 ggctgcttcg atcgccgcgt tcagcgccag cagattggtc tgctcggcaa tttccttgat
     2101 ggtgttggaa atgccatcga tctcatccgc acactggctc agtgtacgaa tgatgtcggt
     2161 ggaatcgcgc accgattgcg ccacaccggc catgccattg gctgcatgtg tcaccacagc
     2221 accgcctttt tcggaaatct cgtgcgcctc gcgcgatgct ttttccgttt cggtggaatg
     2281 atccgccaca tccagaatag tggtagtgat gttctccatg atgtgctggg ccgcctgtgc
     2341 gatctcgtgc tgttccagcg ccgagcggct gacgatttcc gaactcgcct gcagcgtgct
     2401 ggacgccgat gtcagcgccg ttgccgtagc cagcacctgc ttcaccgtat catccagctt
     2461 gtcgacgaaa ctgttgatcc agagcgccaa ttgcccggtt tcgtccttgg gaaacgtatc
     2521 tacgtcgatg cgcgtctgca gtgagctgcc agcttcggcg atatccagaa aaaaattctc
     2581 cagccgctgc aatcgttgcc gcaggggccg caatccaagc agccagaaca ccagcaggat
     2641 caacgcgcca gtgccggcat tcaccagcag cgttgcaccg cccgttacat tcagcaggtg
     2701 cttgcaatat tccgccaccg caaaccctgc gccacccagc aatgccagcc gcgtcagcaa
     2761 cgtcagatca atactgcgct cgcgatacac ctcctccaga tcgccctcgc acaacaggcc
     2821 ccacacatcc atcgatccgg gcattttgaa ggtcaggccc gcgccaatca ccggcacatg
     2881 gcggtaatcg ggatagccag ggtagcagac gaaaacatga tggcggttac gaatggtttc
     2941 ccgtacaccg ggatgcagat cacgggtggc gggatcggta aagcgcagtt ccagttccgt
     3001 tcgctcgcgc acgctcacgg tgccgtaatc cgtgcgcaca ccctgcttca gattgtcacc
     3061 atgggtgaag gcattgtcct cgaaacgcga acgcgacagc gccacaccgg gctgaatcga
     3121 cggatcgaac ttcgattcca ccataaacag gtaattgtcg ccggaatcgc ggtacacat
//
LOCUS       DASUEG010000005         6176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1656656, whole genome shotgun sequence.
ACCESSION   DASUEG010000005 DASUEG010000000
VERSION     DASUEG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656656"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VFX11_00080"
     CDS             <1..1660
                     /locus_tag="VFX11_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937040.1"
                     /translation="YVPIDPANPEERIRFILDDAGVALLLTQSSVAGQIPAGRHKALL
                     LDQMQAELAVQPSHNPEPLADGDSRAYVIYTSGTTGKPKGVLIPHSNVIRLFTATEQW
                     YSFDEHDVWTIFHSFAFDFSVWEMWGALFYGGRAIVVPSRVAKSTEDFFELVCNEGVT
                     VLNQTPSAFTQFIRFDEYTREEKPDLAARLKLRYVIFGGEALDFSALQKWAAYHGVEH
                     PMLINMYGITETTVHVTYHRILEDDLDRRQSLIGRPIPDLDLYILDQHLNPVPIGVPG
                     ELHVGGQGLAHGYLNRTDLTDQRFIPSPFGFGVERDKGLYKRLYKTGDVGRYLPNGVV
                     EYMGRIDHQVKIRGYRIELGEIESTISQCAGVRESVVLAREDVPGDKRLVAYVAADEG
                     ALDLSALKADIRKLLPEYMVPSAYVVLSAFPLTVNGKIDKRALPVPDDAAVPKNEYLA
                     PRNETEEVLAEIWQEVLNLEKVGVRDNFFDIGGHSLLATQVVSRVRERFSVELALSAL
                     FEEPTIENIALHLLQTELAGADDLEMAELLAEIEGLSEEDLKNL"
     gene            1876..>6176
                     /locus_tag="VFX11_00085"
     CDS             1876..>6176
                     /locus_tag="VFX11_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937041.1"
                     /translation="MASLSPEQREALLKQLQKKKSQGAEPRKDAIQVYPRDNNRLPMS
                     YAQQRLWFIDQLQSGSASYNISAALRLKGSLDVEALRMSFDEIVRRHEVLRTTFITED
                     GQGRQVINVHQRWELATISLEPLSRDEQEEVIRARFKGDAHTSFDLINGPLLRTRLIR
                     LSSTEHVMIVSMHHIIADGWSMGVFIREMALLYDAFTQDRPTPLQDLKIQYADYSLWQ
                     REWLAGERLERQLSYWKTRLDGVPVLELPTDYPRPPMHTYQGANLHFKLDVETSRALN
                     EVAKKQGVTLFMTLFAALQVLLHRYSGQDDVCVGTPIAGRVRPELEQLIGCFVNTLAI
                     RSDLSGNPTFTSLLKQMQQNLTGAYDHQDIPFERLVDELGVAREMSHTPLFQVMFVLQ
                     NATTTSALALPGLNIELLPEESETSKFDITINMREEKGCLLGDWEFRTDLFEARTIQR
                     MITHFQNVLKAVAANPSGAIDDFDYLTAQERQQILVDWNNTRSEYERDDDVVEVFNRQ
                     VQQFADRTAVQLGSDKLTYAQLDRQANHVALWLIEKGVKPGQRVGLCAERSLETIAAV
                     LGILKAGAAYVPLDPSYPQDRLHYMLDDTAAQIVIAHPHLTANLPVVADNIIDLNLLR
                     HQTAPCPDPGVRVGPLAPAYVMYTSGSTGKPKGIEVTHRNIVRLVRNTNFMKLDDSVA
                     FLQYAPISFDAATLEIWGPLLNGGRIVVAQPGQLSAEDLGAVIAQGEVNAAWLTAALF
                     HLFAEYHVEQLKPVRQLLAGGDVLSPKMVRKVLDLNPGTTLINGYGPTENTTFTCCYP
                     MRTAQDVRHTVPIGRPIANTRVYVLDSKLKPVPVGVPGELYTSGDGVANGYLNREELT
                     AEVFIENPFRSGDDSRLYKTGDLVRYYPDGNIEYLGRIDQQVKIRGFRIELSEIEDAI
                     SRINTIREVAVIAREDVPRVKRLVAYYVPVEGALPTATELRAELKQTLPDYMVPSAFV
                     AMEHLPVTANGKLDRRALPKPDTDNEAALQRIPPRTEKEKILAQIWCQVLNLEEVGIR
                     DNFFELGGDSILSIQIISRAKLGGLHLTTKQIFENQTIEELAEVASEVETPILAEQGL
                     VVGDVPLSPIQHWFFDHEFAHEQHWNQSVLLDIKPSAKLTEQQIGRALASVVLQHDAL
                     RLRFVRSGDSVRQFHFENADGTSHFAMHPIDLVNAQEADLAAVITARCTDIQASLNLE
                     KGPLLAAGYFKLPEGRRKLLLVVHHLVVDGVSWRILLDDFTQALNRIVQHEDVKLGLK
                     TTSYQQWINALSDYVKNGSLMGEMRHWERSVSGEKPVLPLDDPQGRYTVADTRVHTLI
                     LDEEHTLSLLRDVNRAYRTEINDVLMTALARTFSDWSGNVETLIDLEGHGREIFTDKI
                     DLSRTVGWFTSVYPVRLRYENGQSLQDNLKSIKEQLRQIPNRGFGFGALRYLGSR"
BASE COUNT         1276 a         1821 c         1911 g         1168 t
ORIGIN      
        1 ctatgtgccg atcgacccgg ccaatccgga agagcgcatc cgtttcattc tggacgacgc
       61 gggcgtggcg ttgttgctga cccagagcag cgtggcgggg cagattccgg ccggtcgaca
      121 caaagccctg ctgctggacc agatgcaggc tgagctggcc gtgcaaccga gccataaccc
      181 cgagccgctg gcggacggtg acagccgtgc ctacgtgatc tacacgtcgg gtaccaccgg
      241 caagccgaag ggcgtgctga ttccacactc caacgtgatc cgcctgttca ccgccaccga
      301 gcagtggtac agcttcgacg aacacgatgt gtggaccatc ttccactcct ttgccttcga
      361 cttctcggtt tgggaaatgt ggggcgcgct gttctacggt ggccgtgcca tcgtggtacc
      421 gtcgcgcgtc gccaaatcca ccgaggattt cttcgagctg gtgtgcaacg aaggcgtcac
      481 cgtgctgaac cagacgccgt cggccttcac ccagttcatc cgcttcgacg aatacacccg
      541 cgaagagaag ccggatctgg cggcacggct gaagctgcgt tacgtgatct ttggcggcga
      601 ggcgctggac ttttcggcac tgcagaagtg ggcggcatac cacggtgtcg agcacccgat
      661 gctgatcaac atgtacggca tcaccgaaac caccgtgcac gtgacctatc accgcatcct
      721 ggaagacgac ctggaccgcc gtcagagcct gatcggccgg ccgattccgg acctggatct
      781 gtacatcctg gaccagcatc tgaatccggt gcccatcggc gtgccaggcg aactgcatgt
      841 cggtggtcag ggcctggccc atggctacct gaatcgcacc gacctgaccg accagcgttt
      901 catccccagc ccgttcggct ttggtgtgga gcgtgacaag ggcctgtaca agcgcctcta
      961 caagaccggc gacgtgggcc gctacctgcc caacggcgtg gtggagtaca tgggccggat
     1021 cgaccatcag gtgaagatcc gcggttaccg tatcgaactg ggcgagatcg agtccaccat
     1081 cagccagtgt gccggcgtgc gcgaatccgt ggtgctggcg cgggaagatg tgcccggcga
     1141 caagcgcctg gtggcctatg tggcagcgga tgagggcgcc ctcgacctgt cagccctgaa
     1201 ggccgacatc cgcaagctgc tgcctgagta catggtgccc agcgcttacg tggtgctgtc
     1261 cgcgttcccg ctcacggtga acggcaagat cgacaagcgc gccctgccgg tgcctgacga
     1321 tgcggccgtg ccgaagaatg agtacctggc accccgcaac gagaccgagg aagtgctggc
     1381 ggagatctgg caggaagtgc tgaatctgga gaaggtgggc gtacgcgaca acttcttcga
     1441 tatcggcggc cattccctgc tggcgaccca ggtggtgtcc cgcgtgcggg agcgcttcag
     1501 tgtcgagctg gcgctgagcg ccctgttcga ggaacccacc atcgaaaaca ttgccctgca
     1561 cctgctgcag acggagctgg cgggggctga tgatctggaa atggcggaat tgctcgctga
     1621 aatcgaggga ttgtccgaag aagacctgaa aaatctgtga cgaagacggg ggagtcggaa
     1681 gggagcgctg ggcgcaacct tccgatttca ttaaaatgga cgcatttaat tgatgcgatc
     1741 gcgaggcgga cagtgatgtt ccgcctaaag ttgtttataa cgaactaaaa tagcagaatc
     1801 ccccaaccag cataacaatc ataagaatcg acacgggccc atcgattaat gacagacaag
     1861 aagttactga accgtttggc gagcctttcc ccggaacagc gcgaggcact gctcaagcag
     1921 ctccagaaga agaagagtca gggagccgag ccgcgcaagg acgccatcca ggtctacccc
     1981 cgcgacaaca atcgcctgcc catgtcctat gcgcagcaac ggctgtggtt tatcgatcag
     2041 ctgcagtccg gcagcgccag ttacaacatc tccgcggccc tgcgcctgaa aggcagcctc
     2101 gacgtcgagg ccctgcgcat gagcttcgac gagatcgtgc gccgccatga agtgctgcgc
     2161 accaccttta tcaccgagga cggccagggc cggcaggtga tcaacgttca ccagcgctgg
     2221 gaactggcca ccatcagcct ggagcctctc agccgcgacg agcaggaaga ggtgatccgt
     2281 gcccgcttca agggtgatgc ccacacctcg ttcgacctga tcaacggccc gctgctgcgc
     2341 acccgtctga tccgcctgag cagcaccgaa cacgtcatga tcgtgtcgat gcaccacatc
     2401 attgccgatg gctggtcgat gggcgtgttc atccgtgaga tggcgctgct gtacgatgcc
     2461 ttcacccagg atcgcccgac accgctgcag gatctgaaaa tccagtacgc cgattattcg
     2521 ctttggcagc gcgaatggct ggccggtgag cggctggagc gtcaactgag ttattggaaa
     2581 acccgccttg acggcgttcc ggtgctggaa ctgccgacgg attatccgcg cccccccatg
     2641 cacacctacc agggcgccaa tctgcatttc aaactggacg tggaaacgtc ccgcgcgctg
     2701 aatgaagtgg cgaagaagca gggcgtgacc ctgttcatga cgctgtttgc cgcgttgcag
     2761 gtgttgctgc accgctacag cgggcaggat gatgtttgtg tcggtacacc gattgcggga
     2821 cgggtgcgtc cggaactgga acagctgatc ggctgcttcg tgaatacgct ggcgatccgc
     2881 agcgatctgt cgggcaatcc caccttcacc tcgctgctga aacagatgca gcagaatctc
     2941 accggtgcct acgatcacca ggatattccg tttgaacgtc tggtggacga actgggcgtg
     3001 gcgcgggaaa tgagccatac gccgctgttc caggtcatgt tcgtgctgca gaacgcgacc
     3061 accaccagcg cgctggcgct gccgggcctg aatatcgagt tgctgccgga agaatcggaa
     3121 acctcgaaat tcgacatcac catcaacatg cgggaagaga agggctgcct gctgggggat
     3181 tgggaattcc gcaccgatct gttcgaggcg cgcaccatcc agcgcatgat cacccacttc
     3241 cagaacgtgc tgaaggccgt ggcggccaat ccgtccggcg ccatcgacga tttcgattat
     3301 ctgactgcgc aggaacgcca gcagatcctg gtggactgga acaatacccg cagtgagtac
     3361 gagcgcgatg acgacgtggt ggaggtgttc aaccgccagg tgcagcagtt tgccgaccgc
     3421 actgcggtgc agctgggcag cgacaaactc acctacgcgc aactggaccg gcaggccaac
     3481 catgtggcct tgtggctgat tgaaaaaggt gtgaagcccg gtcagcgcgt gggcctgtgt
     3541 gccgagcgca gcctggaaac cattgccgct gtgctgggaa ttctgaaggc cggtgcggcg
     3601 tatgtgccgc tcgatccgtc ctatccgcag gaccgtctgc actacatgct ggacgatacc
     3661 gctgcgcaga tcgttatcgc gcatccgcac ctgaccgcga atctgccggt ggtggcggac
     3721 aacatcatcg acctgaacct gctgcgacac cagaccgcgc cgtgccccga tccgggtgtg
     3781 cgggtggggc cgctggcgcc ggcgtacgtg atgtacacct ccggttccac cggcaagccc
     3841 aaaggcatcg aagtgacgca ccgcaatatc gtgcggctgg tgcgcaacac caatttcatg
     3901 aagctggacg attccgtggc gttcctgcag tacgcgccga tttcgttcga tgcagcgacg
     3961 ctggaaatct ggggtccgct gctgaatggc ggccgcattg tggtggcgca gccgggccag
     4021 ctgtcggcgg aggatctggg tgcggtcatt gcccagggtg aggtgaatgc tgcctggctg
     4081 accgccgcgc tgttccacct gtttgcggaa taccatgttg aacagctgaa gccggtccgc
     4141 cagttgctgg cgggtggcga tgtgctgtcg ccgaagatgg tgcgcaaggt gctggacctg
     4201 aatccgggca ccacgctgat caacggatac ggcccaacgg aaaataccac cttcacctgc
     4261 tgctatccga tgcgcacggc gcaggatgtt cggcataccg tgccgatcgg cagacctatc
     4321 gccaataccc gcgtctatgt gctggacagc aaactgaagc cggtgcccgt gggtgttccc
     4381 ggcgagctgt acacgtccgg tgacggcgtg gcaaacggct acctgaaccg cgaggaactg
     4441 acggcggaag tcttcattga aaatccgttc cgttccggtg atgactcgcg cctgtacaag
     4501 actggcgatc tggtgcgcta ttacccggat ggcaatatcg aatacctggg ccgtatcgac
     4561 cagcaggtaa aaattcgtgg cttccgtatc gaactgagcg aaatcgaaga cgccatcagc
     4621 cgcatcaaca ccatccgcga agtggcggtg atcgcgcgcg aggacgtgcc tcgggtgaaa
     4681 cgcctggtgg cctattacgt gccggtggaa ggtgccttgc ccaccgccac cgaattgcgt
     4741 gccgaactga aacagacctt gccggattac atggtgccgt cggcgttcgt ggcgatggag
     4801 catctgccgg tcacggccaa tggcaagctg gaccgccggg cattgccaaa gccggacacc
     4861 gacaatgaag ccgcgctgca gcgtattccg ccgcgcaccg agaaagaaaa aatcctggcg
     4921 cagatctggt gtcaggtgct gaatctggaa gaagtgggaa ttcgggacaa ctttttcgaa
     4981 ctgggtggcg actccatcct cagcatccag atcatcagtc gtgccaaact gggcggactg
     5041 catctcacta ccaaacagat ttttgaaaac cagaccattg aagagctggc ggaagtggcc
     5101 agtgaagtgg agacgcccat tctggcggag cagggtctgg tggtaggtga tgtgcccctg
     5161 tcgccgatcc agcactggtt cttcgatcat gaatttgcgc acgaacagca ctggaaccag
     5221 tcagtgttgc tggacatcaa acccagcgcg aaactgaccg agcagcagat cggccgcgcc
     5281 ctggcgtcgg tggtgctgca gcatgatgcg ctgcgcctgc ggttcgtccg cagtggcgac
     5341 agcgtgcgcc agttccattt cgagaatgcg gatggcacca gccatttcgc catgcatccg
     5401 atcgatctgg tcaacgcgca ggaagcggat ctggcagccg tgatcacggc gcgctgtacc
     5461 gatatccagg ccagcctgaa tcttgaaaaa ggcccgctgc tggccgccgg ctatttcaaa
     5521 ttgccggaag ggcggcgcaa gctgctgctg gtggtgcatc acctggtggt ggacggcgtg
     5581 tcctggcgga ttctgctgga tgatttcacc caggcactga accggatcgt gcagcacgaa
     5641 gatgtgaaat tgggcctgaa aaccacgtct taccagcagt ggatcaatgc actgtcggac
     5701 tatgtgaaga acggttcgct gatgggtgag atgcggcact gggaacgctc cgtcagcggt
     5761 gaaaagccgg tgttgccgct ggacgatccg caaggccgct acaccgtagc ggatacccgc
     5821 gtgcacactc tcattctcga cgaagagcat acgctgtcgc tgctgcgcga tgtgaaccgt
     5881 gcctaccgca ccgagatcaa cgatgtgttg atgacggcgc tggcccgcac cttttccgac
     5941 tggagcggca acgttgaaac cctgatcgat ctggaaggtc acggccgcga aatatttacc
     6001 gataaaatcg atctgagccg cacggtaggc tggttcacgt cggtttatcc ggtgcgcctg
     6061 cgctatgaaa atggccagtc gctgcaggac aacctgaaat ccatcaagga acagctgcgc
     6121 cagattccca atcgtggttt tggttttggt gcgctgcgtt acctgggcag ccgcga
//
LOCUS       DASUEG010000006         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2120476, whole genome shotgun sequence.
ACCESSION   DASUEG010000006 DASUEG010000000
VERSION     DASUEG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120476"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /gene="metG"
                     /locus_tag="VFX11_00090"
     CDS             complement(<1..1827)
                     /gene="metG"
                     /locus_tag="VFX11_00090"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX4937042.1"
                     /translation="MSQHTHQRRILVTSALPYANGPIHLGHLVEYIQTDIWVRFQRAR
                     GHEVHYVCADDAHGTAIMLKAEQNGVTPEQQIATVKADHEQDFADFLIRFDNYHSTHS
                     EENREFSSFIYLKNRDGGHIATRPIRQLFDPEKQMFLADRFIKGECPKCSAKDQYGDS
                     CEVCGATYAPTELKNPYSTISGATPVEKESIHYFFKLPDFAAFLKDRERSGRLQPEVA
                     NKLDEWFEAGLNDWDISRDAPYFGFEIPEAPGKYFYVWLDAPIGYMASFKNLCSKNAA
                     LSFDEFWKEGSNTELYHFIGKDIVYFHALFWPAMLEGAGFRKPSAIFAHGFLTVNGQK
                     MSKSRGTFIKARTYLENLNPEYLRYYFAAKLSGSVEDIDLNLEDFSLRVNSDLVGKVV
                     NIASRCAGFINKKFDGRLTASCAEPVLLNEFIDAGDSIAQHYENRDFGRAVREIMALA
                     DRANQYIDEKKPWALAKVEGKDAEVQEVCSVGLNLFRQLMVYLSPILPATSTASAQFL
                     NISGFEWDSREQLLLDHTINAFQPLMTRIEKEKVDAMVDASKENLLAAQTPAAAPATT
                     PKKAASSADGEQVAEISIDDFMKVDLRVARIVNAEHVEGAEKL"
     gene            1990..>2633
                     /locus_tag="VFX11_00095"
     CDS             1990..>2633
                     /locus_tag="VFX11_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX4937043.1"
                     /translation="MTDLAVVKASLNQVKDPLLDQTLAELGWLESVVLDGSTVLVDLV
                     PTYAGAAQELYPLLLEALAQSGVDQLGLRERPAIPRVKPQGTVPALDRVKNVIAVSSG
                     KGGVGKSTTAVNLALALAAEGARVGMLDADIYGPSQPMMLGIPAGTRPGVKDNAWFIP
                     VQAHGIEAMSIGFLLEGDNSPVVWRGPKATGALLQMLQQTLWSDLDFLIIDMPPG"
BASE COUNT          507 a          688 c          847 g          591 t
ORIGIN      
        1 cagcttttcg gcgccttcca catgctcggc gttgacgatg cgcgcgacgc gcagatccac
       61 tttcatgaag tcgtcgatgc tgatttcggc gacctgttcc ccgtcagcgg aagaggcagc
      121 tttcttcgga gtggtagcgg gtgcggcagc aggtgtctgg gcagccagca ggttttcttt
      181 cgaggcatcg accatggcgt ccactttttc cttttcaatt cgggtcatga gtggctggaa
      241 ggcattgatg gtgtgatcca gcagcagctg ttcgcgggag tcccattcaa agccactgat
      301 gttgaggaac tgtgcgctgg cagtactggt ggccggcagg atgggggaga gatacaccat
      361 caactggcgg aacaggttga ggccgaccga gcacacttcc tgcacttcag cgtctttgcc
      421 ttctaccttg gccagtgccc aaggcttctt ctcgtcaatg tactggttgg cacgatcggc
      481 cagcgccatg atttcgcgaa cagcgcggcc gaaatcacgg ttttcataat gctgggcgat
      541 gctgtcaccg gcatcgatga actcgttcag caagaccggt tcggcgcagc tggcagtgag
      601 gcggccgtca aacttcttgt tgatgaagcc ggcgcagcgg ctggcgatgt tgaccacctt
      661 accgaccaga tcggaattca cgcgcaggct gaagtcttcg aggttgaggt caatgtcctc
      721 gacagaaccc gacaacttgg cggcgaagta gtagcgcaga tattccggat tcagattctc
      781 cagataggtg cgggccttga tgaaggtgcc gcgcgacttg gacatcttct ggccgttcac
      841 cgtcaggaag ccgtgcgcaa agatggcgct gggcttgcgg aaaccggcac cttcgagcat
      901 ggcgggccag aacagggcgt ggaagtacac gatgtctttg ccgatgaagt ggtacagctc
      961 ggtgttgctg ccttctttcc agaattcgtc aaagctcaat gctgcattct tgctgcagag
     1021 attcttgaag ctggccatgt aaccaatggg ggcgtccagc cacacataga aatacttccc
     1081 gggtgcttcc ggaatttcaa aaccgaaata gggcgcgtcg cgggaaatgt cccagtcatt
     1141 caggccggct tcaaaccatt cgtcgagctt gttggccact tccggctgca gacggccgga
     1201 gcgttcgcgg tctttcagga aggcagcaaa gtcgggcagc ttgaagaaat agtggatcga
     1261 ttccttttcg acaggtgttg caccggaaat ggtcgagtag gggttcttga gctctgttgg
     1321 agcataggtg gcgccgcaaa cttcgcagga atcaccgtac tggtccttgg cgctgcactt
     1381 agggcattcg cccttgatga agcggtcagc caggaacatc tgtttttccg gatcgaacag
     1441 ctgacggatc gggcgggtgg cgatatggcc gccatcacgg tttttcagat agatgaacga
     1501 cgaaaactcg cggttctctt ccgagtgcgt cgaatgataa ttgtcgaagc ggatcaggaa
     1561 gtcggcgaag tcctgctcgt gatcggcctt gaccgtagcg atctgctgtt ccggggtaac
     1621 gccgttctgt tcggccttga gcatgatggc cgtgccatgg gcgtcgtcgg cgcagacata
     1681 gtgcacttca tgaccacggg cgcgctggaa gcggacccag atgtcagtct ggatgtattc
     1741 gacgaggtgg cccaggtgga tggggccgtt ggcatagggc agggcgctgg tgacaaggat
     1801 gcgccgctga tgggtgtgtt ggctcatgac tcggacaatg tcgtggctgg aaagggcgta
     1861 atcataccgg aaaacagggg aaaaaccgga ataactgcgt gccattggcg gacgggtttg
     1921 gctggccctg tcacgggtgt ccggctagca tacgtgccct gaccgctttc ctgtttccgg
     1981 atgtacgccg tgaccgatct tgctgttgta aaagcttccc tcaatcaggt gaaagacccc
     2041 ttgctggacc agacgctggc ggagctgggc tggctggaaa gtgttgtgct ggatggcagt
     2101 acggtactgg tggatctggt gccgacctat gcgggcgctg cccaggaact gtatcccttg
     2161 ctgttggagg ctttggccca atcaggtgtg gaccagttgg gccttcgtga gcgcccggcc
     2221 attccgcggg tcaagccaca aggcacggtg cctgcgctgg atcgtgtgaa gaacgtcatt
     2281 gccgtgtcgt cgggcaaggg cggcgttggc aagtccacta cagcggtgaa tctggccctg
     2341 gcactggcgg cggagggtgc gcgcgtgggt atgctggatg ctgacatcta cggccccagc
     2401 caacccatga tgttgggtat tccggccggt acgcgacctg gcgtgaaaga caacgcctgg
     2461 ttcattccgg ttcaggcgca cggcattgag gccatgtcca tcggcttcct gctggaaggg
     2521 gacaattcac cggtggtctg gcgtggcccc aaggccacgg gtgctttgct gcagatgttg
     2581 cagcagacgc tctggagtga tctggacttc ctcatcatcg acatgccacc ggg
//
LOCUS       DASUEG010000007         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1700862, whole genome shotgun sequence.
ACCESSION   DASUEG010000007 DASUEG010000000
VERSION     DASUEG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700862"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VFX11_00100"
     CDS             <1..1069
                     /locus_tag="VFX11_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4937044.1"
                     /translation="YIRLASQLFGDRMIVANATGCSSIYGGNLPTTPWTQNSDGRGPA
                     WSNSLFEDNAEFGLGLRIAWDQQAQIARDYLQQLEPQLGTEIVTALLQADQADEAGIH
                     EQRERIAAAKLKLASINNPLAAPLLDVIDALSKKSVWIIGGDGWAYDIGYGGLDHVLA
                     SGMNVNILVLDTEVYSNTGGQTSKATPRGAVAKFSAGGKATAKKDLARIALTYENVYV
                     AHVAYGAKDVHTLHTFLEAESYDGPSLIIAYSPCIAHGVDLANNHRQQTLAVDSGHWP
                     LFRYDPRRIAQGQNPFKLDSKPPSIDYADFIKSETRFSMLWRSEPARADALLDQARAD
                     VKERFAFYEHLAAATEDKPHE"
     gene            1062..2132
                     /locus_tag="VFX11_00105"
     CDS             1062..2132
                     /locus_tag="VFX11_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX4937045.1"
                     /translation="MNKPTTTAPTTTALSATDLRTEYLGLSLKNPLVPSSSPLTGDFD
                     NARRLEDAGAAAIVLPSLFEEALLHEEEQMTRFLDHQPIGHPEAQSFLPDLPEYASEL
                     DQHVARIERYKESLSIPVIASLNGVTPQGWVRHAKDLQDAGCDALELNLYGIAARRDE
                     TAAEVEARYLQVVKSLLETVSIPLTVKLSAQFSSPVNFIGELENLGVKGVVLFNRFYQ
                     PDIDLASRHVVPRIQLSHSYEALLRIRWIALLRGQVGLGIAGTGGFHTHEDVLKGLMA
                     GADVVHVCSALLREGPDYLRTILFGMSAWMEQEEYSSVQQLKGSLSQRHAANPSAYER
                     ANYAELLGQDALLKVDLKRHRE"
     gene            2286..>3305
                     /locus_tag="VFX11_00110"
     CDS             2286..>3305
                     /locus_tag="VFX11_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937046.1"
                     /translation="MSTRFSSSKILTCLVASLLFACAFYQLPLFSVNQHTYFLHGLAQ
                     AGYGHLAGDWLSHQTDHVPVFSWLVTQVYRMGSPWLFQALFGLLASLYAASLLLIASS
                     AAGPSAAPQSWQSRLQFVILLLALLTALHSTWMLAPLRDIHAGAADPVALIHRLASVA
                     SYGVAGQTLLGHYLQPSSFGVLLLASIACFLLRRDYIAVVCALVAASLHPTFVLHAGI
                     LTVAYMLVLAREGRPRQAFALGLLALLLVLPIVLYVMAKMQPDSPASLARANAILANE
                     RIPHHTQISVWLSRGTWLKLAIVLAGILAARHNRRLFLVLSFCAAISIALSLLQWISG
                     SNGFAL"
BASE COUNT          630 a         1090 c          973 g          612 t
ORIGIN      
        1 ctacatccgg ctggcctcgc aactgttcgg cgaccgcatg atcgtcgcca acgccaccgg
       61 ctgctcgtcg atttacggcg gcaacctgcc caccacaccc tggacccaaa acagcgacgg
      121 ccgtggcccg gcctggagca attccctgtt cgaagacaac gccgaattcg gcctgggcct
      181 gcgcatcgcg tgggaccagc aggcgcagat tgcccgcgac tatctgcagc agctggaacc
      241 gcaactgggc actgaaatcg tcaccgcact gctgcaggcc gatcaagccg atgaagccgg
      301 cattcacgaa cagcgcgaac gcatcgcagc agcgaaactg aaacttgcca gcatcaacaa
      361 tccgctggca gcaccgctgc tggacgtgat cgatgccctc agcaagaaaa gcgtgtggat
      421 catcggcggc gacggctggg cctacgacat cggttacggc ggcctcgacc acgtgctggc
      481 gtccggcatg aacgtgaaca ttctggtgct cgacaccgag gtctattcca acaccggcgg
      541 ccagacctcg aaagccacac cgcgcggcgc cgtggcgaaa ttctccgccg gcggcaaagc
      601 cacggcgaaa aaggatctgg cccgcatcgc cctgacttat gaaaatgtct atgtggcgca
      661 tgtggcctac ggcgcgaaag acgtgcacac gctgcacacg tttctggaag cggaaagcta
      721 cgacggcccg tcgctgatca tcgcctactc gccctgcatc gcccacggcg tcgacctggc
      781 gaacaatcac aggcagcaga cgctggcggt ggacagcggc cactggcccc tgttccgtta
      841 cgacccccgc cgcattgcgc aggggcaaaa cccgttcaaa ctcgacagca agccgccgtc
      901 catcgactac gcggatttca tcaagagcga aacccgcttc agcatgttgt ggcgctccga
      961 acctgcccgc gccgacgccc tgctggatca ggcccgcgcc gacgtgaagg aacgttttgc
     1021 cttttacgaa cacctggctg cggccactga ggacaagccc catgaataag ccgaccacaa
     1081 ccgcgccgac cacaacggct ctttctgcaa cagacctgcg caccgagtac ctgggcctta
     1141 gcctgaaaaa tccgctggtg ccgtcctcat cgccgctcac cggtgatttc gacaacgcgc
     1201 gccggctgga ggatgccggt gccgccgcca tcgtgctgcc atccctgttc gaggaagccc
     1261 tgctgcatga agaggaacag atgacacgct tcctcgatca ccagcccatc ggccatcccg
     1321 aggcacagtc gtttctgccg gacctgccgg aatacgcctc cgaactggac cagcatgtgg
     1381 cccgcatcga gcgttacaag gaatccctgt caattccggt gattgccagc ctcaatggcg
     1441 tcacaccgca aggctgggtg cgccacgcga aggatctgca ggacgccggc tgcgacgcgc
     1501 tggaactgaa cctgtacggc atcgccgccc gccgcgatga aacagcagcg gaagtagagg
     1561 cccgctacct gcaggtagtc aaatcactgc tggaaacagt gtcgattcct ttgacagtca
     1621 agctgtcggc gcagttttcc tcgccggtga atttcattgg tgaactggaa aacctggggg
     1681 tgaaaggggt ggtgctgttc aaccgtttct accagccgga catcgacctg gcgtcccgtc
     1741 atgtggtgcc gcgcatccag ctgtcccatt cgtatgaagc actgttgcgc atccgctgga
     1801 ttgccctgct gcgcggccag gtggggctcg gcattgccgg caccggcggc tttcacactc
     1861 atgaagatgt gctcaaaggc ctgatggcag gcgctgatgt agtgcatgtg tgcagcgcgc
     1921 tgctgcggga aggcccggac tacctgcgta cgatcctgtt cggcatgtcc gcctggatgg
     1981 aacaggagga atacagctcg gtacagcagc tgaaaggcag cctgagccaa cgccatgcag
     2041 cgaatccttc ggcctatgag cgcgccaact atgcggaact gctggggcag gacgcactgc
     2101 tgaaagtcga cctgaagcgg caccgggaat gacaaatgca tgctaattgg acatcgccat
     2161 ggcgccgcaa cagcgccggc tggctggacg caagcaacca caacgggcag agtaggcaac
     2221 ccggtgggga cgcccaagcc ggcgcgctgt gacgctgcat ctgaagttcc gtattaaggt
     2281 aaagaatgag cacccgtttt tcatccagca agatcctgac ctgtctcgtg gccagccttc
     2341 tgtttgcctg tgctttctat cagctgcctc tgttcagcgt gaatcagcac acttactttc
     2401 tgcacggtct ggcgcaggcc gggtacggcc atctggcggg tgactggctc agccaccaga
     2461 ccgaccacgt gccggtgttt tcctggctgg taacccaggt gtaccgcatg ggcagtccgt
     2521 ggctgttcca ggctctgttc gggctgctcg ccagcctgta tgccgcgtcc ctccttctga
     2581 tcgcctccag tgccgccggg ccgtccgcag caccgcaatc ctggcaatcc cggctgcaat
     2641 tcgtaatcct gttgctggcc ctgctgacag cgctgcacag cacctggatg ctggccccgc
     2701 tgcgggacat ccacgcgggg gccgctgatc cggtcgccct cattcaccgg ctggccagcg
     2761 tggccagcta tggcgtggcc ggtcagaccc tgctgggaca ctatctgcaa ccctccagct
     2821 ttggcgtact gctgctggcc agcatcgcct gttttctgct gcgccgggac tacatcgccg
     2881 tcgtctgcgc cctggtggcg gccagcctgc accccacctt cgtgctgcac gccggcattc
     2941 tcacggtcgc gtacatgctg gtgctggcaa gggaaggacg cccccggcag gcgttcgcac
     3001 tgggtctgct ggcactgctg ctggtgctgc ccatcgtgct gtatgtcatg gcgaagatgc
     3061 agcccgacag ccccgcatcg ctggcccggg ccaatgccat tctggcgaat gaacgcattc
     3121 cccatcacac ccagatatcg gtgtggctgt cacggggtac ctggctgaaa ctggccatcg
     3181 tgctggcggg tatcctggcc gccagacata accggcgcct gtttctggta ctgtccttct
     3241 gcgccgccat cagcatcgcg ctcagcctgt tgcagtggat atcgggcagc aatggattcg
     3301 ccctg
//
LOCUS       DASUEG010000008         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_198377, whole genome shotgun sequence.
ACCESSION   DASUEG010000008 DASUEG010000000
VERSION     DASUEG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198377"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="VFX11_00115"
     CDS             <1..2016
                     /locus_tag="VFX11_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4937047.1"
                     /translation="VTAGIRELGFTPELLTGLPSSAPGQAAEQVPGASHSRWALLALS
                     GVAAVAAEASEWLDAPHWLAALLALVAILSVGLGTYKKGWIALKNRNLNINALMSIAV
                     TGAMLIGQWPEAAMVMFLFTVAELIEARSLDRARNAIRGLLDLTPQQASMRQDDGQWR
                     AVATQAVPVGAIVRVLPGERIGLDGTVVAGRSTVNQAPITGESLPVEKGPDEPLFAGT
                     INQTGVLEFRVTAIAADTTLARIIHAVEEAQGARSPTQRFVDRFSRIYTPVVLALALL
                     VAIAPPLYLAPADMQVWLDWIYRALVLLVIACPCALVISTPVTIVSGLATAARRGILV
                     KGGAFLEMGRNLQVLALDKTGTLTHGKPVQTDAVVLSGEPAVVRGLAASLSRHSHHPV
                     SKAVALAAAGAENRDITGFEDLPGRGVRGRVDGHHYHFGNHRLMEDLGLCSPALEQQL
                     EQFERQGKSVVMLADEQQVLALFAVADTLRPASAAAIQDLHALGIRTLMLTGDNPHTA
                     QAIAAQVGIDAVHANLLPQDKLQQVKDLVEAGTVVGMVGDGINDAPALARANIGFAMG
                     AAGSDTAIETADVALMDDDLRKIPEFLRLSRKTAAVLKQNIAIALGIKLVFLLITLAG
                     VGTLWMAVFADMGASLIVVANGLRLLRRAPDNAPTLSVNPATTVDAA"
BASE COUNT          360 a          656 c          703 g          375 t
ORIGIN      
        1 gtcaccgccg gaatccgcga gctgggtttt acgccggaat tgttgaccgg cttgccgtcg
       61 tccgccccag ggcaggcagc ggagcaggta cctggcgcca gtcactcccg ctgggccttg
      121 ctggcgctgt ccggcgtcgc cgcggtggcg gcagaagcgt cggaatggct ggacgctccg
      181 cactggctgg cggcgctgct ggcgctggtg gccatcctca gcgtcggcct gggcacctac
      241 aaaaagggct ggatcgccct gaaaaaccgc aacctcaaca tcaacgccct gatgagcatc
      301 gccgtcactg gcgccatgct gatcggccag tggcccgagg ccgccatggt gatgttcctg
      361 tttaccgtgg cggaactgat cgaagcccgt tcactggacc gcgcccgcaa tgccattcgc
      421 ggcctgctgg acctgacgcc gcagcaggcc agcatgcggc aggacgatgg ccagtggcgg
      481 gcggtggcca cccaggccgt gcccgtgggg gccatcgtgc gggtgctgcc tggcgagcgc
      541 atcgggctgg atggcaccgt cgtggcggga cgttccaccg tgaatcaggc ccccatcacc
      601 ggcgagagcc tgccggtgga gaaagggccg gatgagcccc tgtttgccgg caccatcaac
      661 cagaccggcg tgctggaatt ccgcgtcacc gccatcgccg ccgacaccac cctggcccgc
      721 atcattcatg cggtggaaga ggcccagggt gcccgttcac ccacccagcg tttcgtggac
      781 cggttttccc gcatctacac gccggtggta cttgcactgg cattgctggt tgccattgcg
      841 ccgccgcttt atctggcacc ggccgacatg caggtctggc tcgactggat ttaccgcgcc
      901 ctggtgttgc tggtgattgc ctgcccctgt gcgctggtga tctccacgcc ggtcaccatc
      961 gtcagcggac tggcgacagc agcgcgacga ggcattctgg tgaaaggcgg ggcgtttctg
     1021 gagatgggaa ggaatctgca ggtgctggcg ctggacaaga ccggcaccct gacccacggc
     1081 aaaccggtgc agaccgatgc ggttgtgctg tccggagaac cggccgtggt acgagggctg
     1141 gcagccagcc tgtcccgtca ttcccaccat cctgtttcca aagctgtcgc actggcggcg
     1201 gccggcgctg aaaatcgcga cattaccggg tttgaagatc tgccggggcg cggtgttcgc
     1261 ggccgggtgg acggccatca ttaccacttc ggcaaccacc gcctgatgga ggatctgggg
     1321 ctgtgttcgc ccgcactgga acagcagctg gagcagttcg aacggcaggg caaatccgtg
     1381 gtgatgctgg cggatgagca gcaggtgctg gccctgtttg ccgtggcgga taccctgcgc
     1441 cctgccagcg ccgccgccat tcaggatctg catgcgctgg gcatccgcac cctgatgctc
     1501 accggcgaca acccgcatac cgcacaggcc attgccgcgc aggtgggcat cgacgccgtg
     1561 cacgcgaatc tgctgccaca ggacaaactg cagcaagtga aggatctggt ggaagcgggc
     1621 acggtggtgg gcatggtggg cgacggcatc aacgatgccc cggccctggc ccgagcgaat
     1681 atcggttttg ccatgggcgc cgccggcagc gacaccgcca tcgaaaccgc agacgtggca
     1741 ctgatggatg acgacctgcg caagattccc gaattcttgc ggctgtcgcg caaaacggcg
     1801 gcggtgctga agcagaacat tgccatagcg ttgggcatca agctggtgtt cctgctgatt
     1861 acgctggccg gcgtcggtac attgtggatg gccgtgtttg ccgacatggg tgccagcctg
     1921 atcgtggtgg cgaatggatt gcggctgtta aggcgggcac ctgataacgc gcctacgctg
     1981 tcggtgaatc cggcaacaac cgtggatgct gcgtaatgaa caccatcacc aaaactgcgt
     2041 ttctgaagga aatggcgctg gcggaaatct gctggcaaca gcgtgcgctg gagc
//
LOCUS       DASUEG010000009         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1921337, whole genome shotgun sequence.
ACCESSION   DASUEG010000009 DASUEG010000000
VERSION     DASUEG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921337"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFX11_00120"
     CDS             <1..673
                     /locus_tag="VFX11_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX4937048.1"
                     /translation="LTTYSATKSFMDMFSDDPDFTQWLAVWLYEETKHPHALIKWLHM
                     VGAEVDSSFLMQGREITPMTPSKAEMLAFNIISEIVAGHTYTQTAALMDEPVLKQIAM
                     CLAKDELRHSVGFENYARQLIKNSEDPDKDKIICLRAAWAFLQDEGFISHPVFLTVRN
                     LGGIIGGEEVIRKIRKAVASRIGKLVGVEIPEPEMIFDVYSEFKRNYRKNRAAGASAL
                     AAESV"
     gene            673..1401
                     /locus_tag="VFX11_00125"
     CDS             673..1401
                     /locus_tag="VFX11_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4937049.1"
                     /translation="MTATLIELDAVSKQYALGESLVLALDDVSLGIGAGEFAAVWGPS
                     GSGKSTLLNLIGLIDQPSKGAVRIEGQDTHALSDRALTDIRFRTLGFVFQNYNLVPVL
                     SALENVMLPLQIRGAAEKPARVKAEALLEAVGLSQQMHSRPDKMSGGQRQRVAVARAL
                     VTDPRIVLADEPTANLDSRNSQIIIDLMRDLNQSRGVTFVFSTHDQQLLDHVDRHIHM
                     RDGKIIGDRKCTPAAAAKAVLAPA"
     gene            1431..2678
                     /locus_tag="VFX11_00130"
     CDS             1431..2678
                     /locus_tag="VFX11_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4937050.1"
                     /translation="MKASVVYFKLGLRNILKNRRRSFVTLFSLAAGFSAIALFTGYTS
                     YVYQGLALHAISGEMLGHLTVAKKGYALGSQMDPERYFFSSDELSRLTDTLKTDSNVD
                     FVSPRISIQGLFSNGTASVVFIGEGIVAEHVNRVRKAIAEESGLPLPPLKMDNEASAI
                     VATDLARIMGVKPGDSAVLFGSSLAGMANAVDVDVADTVNTGAQATNDKYLLIPFRHA
                     QSLLDTDGASRVQVMLKDPSEIDQQRSRVEAMLKNAGLDVEVNTWVEMSNSYQPIRNL
                     FNMIFLFIFSIVLTISITSVINTMGMVVVERTREIGTLRALGMHRFQVINLFSVEGML
                     LALLGGVIGTGTSVLLAQLVNRLDMSYVPPNFSEKVALQVDIQLPQLAVIFVFFLLLG
                     TLAALVPAWNASKRRIVDALGHV"
BASE COUNT          589 a          772 c          849 g          592 t
ORIGIN      
        1 gctcaccacc tattccgcca ccaagtcgtt catggacatg ttctcggacg atccggattt
       61 cacccagtgg ctggcagtct ggctgtatga ggaaaccaag catccgcacg cactgatcaa
      121 gtggctgcac atggtaggcg ccgaagtgga cagcagcttc ctgatgcagg gtcgcgaaat
      181 cacgccgatg acgccgtcca aggctgagat gctggcattc aacatcattt ccgaaatcgt
      241 ggcgggccat acctacaccc agaccgctgc gttgatggac gagcctgtgc tcaagcagat
      301 tgcaatgtgt ctggccaagg atgagctgcg ccactccgtt gggttcgaga attacgcgcg
      361 tcaactgatc aagaactccg aagatccgga caaggacaag atcatctgcc tgcgtgcggc
      421 ctgggcattc ctgcaggacg aaggcttcat cagccaccct gtgttcctga ccgttcgcaa
      481 tctgggcggt atcatcggtg gagaagaagt gatccgtaaa atccgcaagg ccgttgccag
      541 ccgtatcggc aagctggtgg gtgtggagat tccggagccg gaaatgattt tcgatgtcta
      601 cagcgagttc aagcgcaact accgcaaaaa tagagcggca ggtgcttcgg cgctggcggc
      661 agagtcagtc tgatgacagc gacactgatc gagcttgacg ccgtcagcaa gcagtatgca
      721 ctgggcgaaa gcctggtgct ggcgctggac gatgtcagcc tgggaatcgg tgctggcgaa
      781 tttgctgcgg tctggggccc gtcaggcagc ggcaaatcca cgctgttgaa cctgattggc
      841 ctgatcgatc agcccagcaa gggtgcggtc cgcattgaag ggcaggacac gcatgccctg
      901 tcggaccgcg cactgaccga tatccgtttc cgcacgctgg gattcgtgtt ccagaactac
      961 aatctggtgc ccgtgctcag cgcgctggaa aacgtgatgc tgcccctgca gattcgcggg
     1021 gctgccgaaa agcctgcccg cgtgaaagca gaagcgttgc tggaggccgt cggtctcagc
     1081 cagcagatgc attcgcgtcc ggacaagatg agcggaggcc agcgccagcg tgtagccgtg
     1141 gcgcgggcgc tggtgaccga tcccaggatc gtgctggcag atgagcccac cgccaatctg
     1201 gattcgcgca acagccagat catcattgat ctgatgcgtg acctgaacca gtcccggggt
     1261 gtcaccttcg tgttctccac ccatgatcag cagttgctgg atcatgtgga cagacacatt
     1321 cacatgcgtg atgggaagat catcggcgac agaaaatgca caccggcagc agcggcaaag
     1381 gccgttctgg cgccggcgta actggcagaa gatactgaac gggacagcct gtgaaagcga
     1441 gtgtggtgta tttcaaactg ggattgcgca atatcctcaa aaaccggcga cgcagttttg
     1501 tcacgctgtt ctcgctcgcc gccggatttt ctgccattgc gcttttcacc ggctatacct
     1561 cctatgtgta ccagggcctc gcgctgcatg cgatcagtgg ggaaatgctg ggacatctga
     1621 ccgtagccaa gaagggctat gccctgggat cgcaaatgga tcctgaacgc tattttttct
     1681 ccagtgacga actgagccgg ctcaccgaca cactgaaaac ggattccaat gtggatttcg
     1741 tctcgccgcg catcagcatt cagggcctgt tttccaacgg cactgcgtcg gtggtgttta
     1801 ttggcgaagg tattgtggct gaacatgtca accgggtgcg caaagccatt gccgaggaat
     1861 cgggtttacc gctgccaccg cttaagatgg acaacgaggc atccgcgatc gtggcgacgg
     1921 acctggcgcg catcatgggc gtgaagcctg gcgacagtgc agtactgttt ggatcgtccc
     1981 tggcgggcat ggccaatgca gtggatgtgg atgtggccga cacggtgaat accggcgccc
     2041 aggccaccaa cgacaagtat ttgctgattc cgttccggca cgcccaaagc ctgctggata
     2101 ccgatggcgc ttcgagggtg caggtgatgt tgaaggaccc ttccgagatt gaccagcaac
     2161 gcagccgggt agaagcgatg ctgaaaaatg ctggcctgga tgtggaagtg aacacctggg
     2221 tggaaatgtc gaattcctac cagcccatcc gcaacctgtt caatatgatc ttcctgttca
     2281 tcttcagcat cgtactgacc atctccataa ccagcgtcat caacaccatg ggcatggtcg
     2341 tggtggagcg cacgcgggag attggcacgt tgcgcgcgct gggcatgcat cgcttccagg
     2401 tcatcaacct gttttcggtg gaaggcatgc tgctggcact gctgggaggt gtcataggta
     2461 cgggcacctc tgtgctgctg gcgcaactgg tgaaccggct ggacatgtcc tatgtgccgc
     2521 ccaatttttc cgagaaggtt gcgctgcagg tggatataca gctgccgcag ctggcagtga
     2581 tattcgtgtt ctttctgctg ttgggaacac tggctgcgct ggtgccggcc tggaatgcgt
     2641 cgaaaaggag aatcgtggat gcgctgggcc atgtctgatt ttacattgtc gtcgcagttg
     2701 cgcggcctgt tgatcctgct gatgttgttc ccgtggcctg tcgccatcgc cagcgctcaa
     2761 acacccgacg ccgccacaat cctggcagcc agcgacaagg ca
//
LOCUS       DASUEG010000010         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2054444, whole genome shotgun sequence.
ACCESSION   DASUEG010000010 DASUEG010000000
VERSION     DASUEG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054444"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFX11_00135"
     CDS             <1..471
                     /locus_tag="VFX11_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937051.1"
                     /translation="QGVFLKRQDEVADHFARLTTMEMLSIRHIMVEILTGPRSDRTRQ
                     LIKKHMRPLLDSSVVRTATQMALGVQGYMELKDTIADKAVQMTMGPISEPAFNRDRAK
                     IIERIFSERMRALTSGEFQDLLRPAFQEDEWIFVVLGAVTGLIAGSIQLILGFH"
     gene            complement(611..1306)
                     /gene="ung"
                     /locus_tag="VFX11_00140"
     CDS             complement(611..1306)
                     /gene="ung"
                     /locus_tag="VFX11_00140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4937052.1"
                     /translation="MSASASSIKMEDSWKQRLLPEFSKPYMVQLREFLQQQKNAGKVI
                     YPKGTEYFNAFNLTPFDKVKVVILGQDPYHGPNQAHGLCFSVRHGVAFPPSLLNIFKE
                     IERDLKIPMPHHGCLSHWAEQGVLLLNAVLTVEQAKAAAHQGKGWEQFTDAVIRELND
                     HREGIVFMLWGSYAQKKGQFIDRKKHCVLQAPHPSPLSAHRGFIGCGHFSKANEYLKK
                     RGEKAIDWVLPEA"
     gene            complement(1314..1991)
                     /locus_tag="VFX11_00145"
     CDS             complement(1314..1991)
                     /locus_tag="VFX11_00145"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4937053.1"
                     /translation="MSDESAINYRGRKAARARSEQRRRAILEAALRIVVSEGVRGVRH
                     RAVAKEAGVPLSATTYYFDDISDLIADTFTLFAESAMSDVVDPIYQHVGDFVNQHKDA
                     MHTDPELLDQLLDRLTALIISLLQMELRDKRDHMLAEQAFMHECLRDPRLREVARTYF
                     DHLLEKLVQLCRLIGVEEPEIAGEVLMGTVFRIEQEALMAPADQFNIQKAHKMLKRQL
                     KSLIPDN"
BASE COUNT          458 a          648 c          593 g          464 t
ORIGIN      
        1 cagggggtgt tcctgaaacg ccaggacgaa gtggcagatc attttgcccg cctcaccacc
       61 atggaaatgc tgtccatccg ccacatcatg gtggaaatcc tcaccggccc gcgctcggac
      121 cgcacccgtc agctgatcaa aaagcacatg cggccgctgc tggattccag cgtggtgcgc
      181 acggccactc aaatggcgct gggcgtgcag ggctatatgg aattgaagga cactatcgcc
      241 gacaaggcag tgcagatgac catggggccc atttcggagc cggccttcaa tcgtgatcgc
      301 gccaagatca tcgagcggat tttctcggaa cgcatgcgcg cgctgacgtc tggcgaattc
      361 caggatctgc tgcgaccggc atttcaggag gatgaatgga ttttcgtggt gctgggcgcg
      421 gtgacagggc tgatcgccgg gtcaattcag ttgattctgg gcttccattg agggacgagc
      481 gaacatcggg gcctgcagag cgagacacgc tgcaagtaca tccatgtaag ctcggctgcg
      541 gccatccatg gccgcagatg gtctcgctct gcaagccctg atgtccgctc gctcaagccc
      601 cgatgctgct tcaagcctcc ggcaacaccc aatcgatggc tttctctccg cgctttttca
      661 gatattcgtt ggccttggaa aaatgcccgc agccaatgaa gccgcgatgg gccgacagcg
      721 gcgatggatg cggcgcctgc aacacacaat gcttcttgcg gtcgatgaac tggcccttct
      781 tttgcgcgta actgccccac agcatgaaca caatgccttc gcgatgatcg tttaactcac
      841 gaatcactgc atcggtaaac tgctcccaac ccttgccctg atgcgccgcc gctttcgcct
      901 gctccaccgt cagcaccgca ttcagcagca gcacgccctg ctccgcccag tggctaaggc
      961 agccatgatg gggcatggga attttcaggt cgcgctcgat ttccttgaag atgttcagca
     1021 gcgacggcgg aaacgcaacg ccatgccgca cggaaaaaca caaaccgtgc gcctgattcg
     1081 ggccgtgata cggatcctgt cccaggatca ccacttttac cttatcaaat ggtgtcaggt
     1141 tgaaagcgtt gaaatattcg gtgcctttcg ggtaaatcac cttgccggca tttttctgct
     1201 gctgcagaaa ctcgcgcaac tggaccatgt acggcttgct gaattccggc agcaggcgct
     1261 gcttccagct gtcttccatt ttgatgctgc tggcactggc agacatgaca acctcaatta
     1321 tcgggaatca acgacttcaa ctgacgcttc agcatcttgt gcgccttctg gatattgaac
     1381 tgatccgccg gcgccatcag cgcctcctgc tcgatgcgaa acacggtgcc catcagcacc
     1441 tcgccggcaa tttccggttc ttccacaccg atcagccgac acagctgcac cagtttttcc
     1501 agcagatgat caaagtaggt ccgtgccact tcccgcagac ggggatcgcg caggcattcg
     1561 tgcatgaacg cctgctccgc cagcatgtga tcgcgcttgt cgcgcagttc catttgcagc
     1621 agggaaatga tcagggcggt aagacgatcc agcaactggt ccagcaattc cggatctgtg
     1681 tgcattgcgt ccttgtgctg attgacgaaa tcgccgacgt gctgatagat cggatccacc
     1741 acatcgctca tggcgctttc ggcaaacagc gtgaaggtat cggcaatcag atcggagatg
     1801 tcatcgaaat aataagtggt ggctgacagc ggcacgcccg cctcttttgc caccgcccga
     1861 tgacgcacgc cgcgcacgcc ttcgctcacc acgatgcgta acgccgcctc cagaatggcg
     1921 cgccgccgct gttcgctgcg ggccctggct gctttccttc cccgatagtt gatggcgctt
     1981 tcgtccgaca taaatcctct ggccttgatg cttgagcaag gcggaatcat aacctaatcg
     2041 gacaagcaat tcatggactt gggaaggggg tattaacgga gggcaggcaa aaaaaggccc
     2101 cgcgggcttt tgacggcgcc gaggggctcc cagcaagaaa acgagacaga ttgtttttgt
     2161 tcg
//
LOCUS       DASUEG010000011         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1524044, whole genome shotgun sequence.
ACCESSION   DASUEG010000011 DASUEG010000000
VERSION     DASUEG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524044"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1216)
                     /gene="recF"
                     /locus_tag="VFX11_00150"
     CDS             complement(137..1216)
                     /gene="recF"
                     /locus_tag="VFX11_00150"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX4937054.1"
                     /translation="MSVVRLEIAHLRNLQSVSLLPHPHLNLLTGANGSGKTSVLEALH
                     LLGLGRSFRSGRARRLVNDAESACTVFAQFADGGQAGIRRGANGETALKVDNSSQVTL
                     AQLVHRLPLCLLDPESMDLLDAGSKPRRAQLDWGVFHVEHRFYPVWLRYQRALKQRNS
                     LLRSGNIGRLESAVWHREMAESARQLHAFREDYLARWQPQWEARIAAFLPDQSLSLEY
                     TPGWDVALPLEDQLAESWERDVDRGHTQIGPHRADLRVKRGTAPADEVLSRGQKKLVV
                     CALKLSQVALLQATGQQCVLLVDDLASELDALARQRLIDVLAVSGAQVFITSIEPEAV
                     RPQLESTGNSFKMFHVEHGTVVEVP"
     gene            complement(1246..2349)
                     /gene="dnaN"
                     /locus_tag="VFX11_00155"
     CDS             complement(1246..2349)
                     /gene="dnaN"
                     /locus_tag="VFX11_00155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX4937055.1"
                     /translation="MKLSISRDQLLKPLQQVSGVVEKRQTLPVLANVLMVVDAGKLAL
                     TGTDLEVELIARTELAGEFGDGEITVPARKLLDICKSLPASAEIELKQDGQKLSLKSG
                     KSRFSLATLPANEFPNLEEGVGAFSLTITQKALKKLIDKTSFAMAQQDVRYYLNGMLF
                     EVTAGRLRTVATDGHRLAMCDAEAQTDSVDRMQVIVPRKGVHELARLLNDDDGAVTIT
                     LGSNHIRATVGNVTFTSKLIDGKFPDYERVLPKGGDKTVLANREELRQSLTRAAILSN
                     EKFRGIRLQLTPDLLRIQANNPDQEEAEEDVTVEYNGASLEIGFNVQYLMDVLGVLDN
                     ATAKFTLGDSNSSALIQDAEGGSSVYVVMPMRL"
     gene            complement(2379..3731)
                     /gene="dnaA"
                     /locus_tag="VFX11_00160"
     CDS             complement(2379..3731)
                     /gene="dnaA"
                     /locus_tag="VFX11_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX4937056.1"
                     /translation="MAIWPVCLERLQDELPQNLFNMWIRPLQAEEQAGALRLLAPNPF
                     FVRHIADKYLARIRELVKELSQGSMEQVLLEVGNRQDREAPPPVSKPREASPTPTTTV
                     VPESLPRPANNLNPLFTFENFVAGKSNQLAFAACRQVAEKPGMSGHNPLFIYGPTGLG
                     KTHLMHSVGNAVLAANPNARILYLTSDRFVQDFITALQRGLMNDFKRLYRSLDVLLID
                     DIQFFAGKTSTQEEFFHTFNALLEGSRQVILTSDKFPKEINEVDERLKSRFAWGLTLQ
                     VEPPELETRAAILMKKAEQNAIRLPQPSAVFIAQHVQANVRELEGALNKVIATARFKG
                     RDLDLELVRESLKDVLAVRAKQINIDNIQRVVAEYYRIPLRELIGKKRNRSYARPRQV
                     AMALSRELTSHSYPEIGHSFDGRDHSTVIHACNLIDELRKSDPNMDEDYTNLLRLLQA
                     "
     gene            4226..4360
                     /gene="rpmH"
                     /locus_tag="VFX11_00165"
     CDS             4226..4360
                     /gene="rpmH"
                     /locus_tag="VFX11_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX4937057.1"
                     /translation="MKRTFQPSTLKRARTHGFRARMATKNGRTVLARRRAKGRKNLTV
                     "
     gene            4378..4782
                     /gene="rnpA"
                     /locus_tag="VFX11_00170"
     CDS             4378..4782
                     /gene="rnpA"
                     /locus_tag="VFX11_00170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX4937058.1"
                     /translation="MTARYRFPRTHRLLRPAEFQTVFDSTAFKVGEQHFLLLVRPNGL
                     DRPRLGLVIAKKKVRLAVERNRLKRTIRDSFRLHQADLPAVDVIFMARNDLGSLLSAD
                     LATQLDKSWKRLARKAGTPAATPSADVAEPKA"
     gene            4779..5060
                     /gene="yidD"
                     /locus_tag="VFX11_00175"
     CDS             4779..5060
                     /gene="yidD"
                     /locus_tag="VFX11_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX4937059.1"
                     /translation="MKILFILPIRFYRIAISPMMAPHCRFHPSCSAYAEEAIHRHGAV
                     KGMALAVNRILRCHPWAEGGLDPVPEKFSLRRVAPSTDIPDLPRISKAP"
     gene            5064..>5307
                     /locus_tag="VFX11_00180"
     CDS             5064..>5307
                     /locus_tag="VFX11_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions to insert inner membrane proteins into
                     the IM in Escherichia coli; interacts with transmembrane
                     segments; functions in both Sec-dependent and -independent
                     membrane insertion; similar to Oxa1p in mitochondria;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4937060.1"
                     /translation="MELRRTLLIVAMAVVGYMLILTWQKDYGAKPVADAVAATSPAVA
                     PTVANGDTPAVPAAPANGDVPAVPSAPASTDAPVEST"
BASE COUNT         1099 a         1630 c         1472 g         1106 t
ORIGIN      
        1 ctttcctgtt cgctcatcat catctccggg cttccatctg tccgctacgc ctgcgcgctc
       61 cgcggctgac tctgcctctt ctgtatatgg gtcttccacc tcgggagacc cggcttgaag
      121 gccgcaaacc cagtatttac ggcacttcta ctactgttcc atgttccacg tggaacattt
      181 tgaagctgtt ccccgtgctc tccagctggg gcctgacggc ttccggttcg atactggtga
      241 taaacacctg cgcgccggaa accgccagca catcaatcaa gcgctggcgg gcgagggcat
      301 ccagctccga tgccaagtca tccaccagca atacacactg ctgtcccgtg gcttgcagca
      361 aggccacctg cgagagtttg agagcgcaga caaccagctt cttctgcccc cgcgagagga
      421 cttcatcggc cggtgccgtt ccccgcttca cccgcaggtc agcccggtgc gggccgatct
      481 gggtgtggcc gcgatccaca tccctttccc agctttccgc caactggtct tccagcggca
      541 atgccacatc ccaacccggg gtgtactcca gggacaggga ctggtccgga agaaaggccg
      601 cgatacgggc ctcccattgc ggctgccatc gcgccagata gtcctcgcga aaggcatgca
      661 gctgccgcgc cgactccgcc atttcacggt gccagacggc cgattctaac cttccgatat
      721 tacccgatct gagcagggaa ttcctttgtt tgagagcccg ttggtagcgc aaccacacgg
      781 ggtagaagcg atgttccacg tggaacactc cccaatccag ttgcgcccgt cgcggcttgg
      841 atccggcatc cagcaaatcc atggattccg ggtctagcag gcacaggggt aacctgtgga
      901 caagttgtgc aagagtgacc tgggagctgt tatcaacttt cagggccgtt tcgccgttgg
      961 caccacggcg aatccccgcc tgcccgccat ccgcgaactg ggcgaacacc gtacaggcag
     1021 attccgcatc attcaccaac cgacgtgccc gtccggaacg aaaggaacgt cccagaccca
     1081 gcagatgcaa ggcctccagc acagaggtct tgccgctgcc attggcacca gtcaaaaggt
     1141 tgagatgagg gtgggggaga agggagacgg actggagatt gcgcagatgg gcaatctcca
     1201 gtcgaacaac ggacatgagg gaagggggac cgggtcagcc agaccttaga gacgcatcgg
     1261 catgacgacg tagacggagg aaccgccttc ggcatcctgg atcagagcag agctgttgga
     1321 gtcgcccagc gtgaacttgg cggtggcatt atccagaaca cccagcacgt ccatcaggta
     1381 ctgcacgttg aaaccaattt ccagcgacgc gccgttgtac tccaccgtga catcttcttc
     1441 cgcctcttcc tgatccgggt tgttggcctg aatgcgcaac aggtccgggg tcagctgcaa
     1501 gcgaatacca cggaacttct cgttggaaag aatggcggca cgggtcaggg actggcgcag
     1561 ttcttcacgg ttggccagca cggtcttgtc accgcccttg ggcagcacac gctcgtagtc
     1621 cgggaactta ccgtcgatca gcttggaggt gaaggtgaca ttgcccacgg tggcgcggat
     1681 atggttgctg cccagggtga tagtgacggc accgtcgtca tcgttcagca gacgggccag
     1741 ctcatgcaca cccttgcgtg gcacgatcac ctgcatgcga tccacgctgt cggtctgggc
     1801 ctcggcatca cacatggcca gacgatgacc atcggtggcc acggtacgca ggcgaccggc
     1861 agttacctcg aacagcatgc cgttcaggta gtaacgaaca tcctgctggg ccatggcaaa
     1921 ggaggtcttg tcgatcagct tcttcagggc cttctgggtg atggtcaggc tgaacgcgcc
     1981 cacaccttcc tccagattgg ggaattcgtt ggccggcagg gtggccagcg aaaagcggct
     2041 cttgccggat ttcagggaca gcttctggcc atcctgcttc agctcgatct cggcactggc
     2101 cggcagcgac ttgcagatgt ccagcagctt gcgggccggt accgtgatct cgccatcgcc
     2161 aaattcaccg gccagctcgg tacgggcaat cagttccact tccaggtcgg taccggtgag
     2221 ggccagcttg ccggcatcca ccaccatgag gacgttggcc agcaccggca gggtctggcg
     2281 cttttccacc accccggata cctgctggag cggcttcagc aactggtcac gcgaaatcga
     2341 aagtttcatg ggtcggcttc cttggtttca tctggtcatc acgcctgcag caggcgcaaa
     2401 agattggtgt agtcctcatc catgttcgga tcggacttgc gtaattcgtc gatcaggtta
     2461 caggcatgga tgaccgtgga atgatcacgg ccatcaaagc tgtgcccgat ttcggggtaa
     2521 ctgtgcgacg tcagttcgcg tgacagggcc atcgccacct gacgcggacg cgcataggaa
     2581 cggttacgtt tcttgccgat cagttcgcgc aagggaatgc ggtaatactc cgcaaccacg
     2641 cgctggatgt tgtcgatgtt gatctgcttg gcacgtaccg ccagcacatc cttgagcgat
     2701 tcccgcacca gttcgagatc gagatctcgt cccttgaaac gggcggtggc aatcaccttg
     2761 ttgagtgcac cttccagttc acgcacgttt gcctgcacat gttgtgcaat gaaaactgcc
     2821 gaaggttgcg gcaaacggat cgcattctgt tcggcctttt tcatcaggat ggccgcacgc
     2881 gtttccaact ccggcggctc tacctgcagc gtcagtcccc acgcaaaacg ggacttgaga
     2941 cgctcatcca cctcattgat ttctttcgga aatttgtcgg atgtcaggat cacctgacga
     3001 gagccttcga gcaaggcatt gaaggtgtgg aagaactctt cctgtgtgct ggtcttgccg
     3061 gcaaaaaact ggatgtcgtc aatcagcagc acatcgagcg agcgataaag ccgtttgaaa
     3121 tcgttcatca aaccgcgttg caacgccgtg atgaaatcct gcacgaaacg atccgacgtg
     3181 agatacagga tgcgcgcatt cggattggcg gccagcaccg cattgcccac cgaatgcatc
     3241 agatgggttt tacccaagcc ggtggggcca tatataaaaa gcggattgtg accactcatg
     3301 cccggctttt cggctacctg ccggcacgcc gcaaacgcca gttggttgga tttgccggcc
     3361 acaaagtttt caaaggtgaa caagggattc agattgttcg ccggacgcgg caacgattcc
     3421 ggcaccaccg ttgtagtcgg tgttggcgaa gcctcacggg gtttactgac cggtggcggt
     3481 gcttcgcgat cctggcgatt gccgacttcc agcaacacct gctccatgct gccctggctc
     3541 aattccttca ccagctcgcg gatacgagcc aggtatttgt cggcaatatg gcggacaaag
     3601 aaaggattgg gcgccagcaa tcgcagggca ccagcctgtt cttcggcctg aaggggccgt
     3661 atccacatat tgaagagatt ctgaggcaac tcgtcctgca gacgctccag acacacgggc
     3721 cagatcgcca ctagatcatc ctgaaaacag caaagcaaaa ggggcgcgat tttatccaca
     3781 aggcagggca gccgccagca ccggcaaaaa aatccacctt gccagtccat tttcaggcct
     3841 gtcagagaac tgtggaaaac cggcagggca acgccggtac aaacccgggg ataaatcctg
     3901 atgcctaagt tatccacaac tggccacagc ttttcaagtg ccagacagct caccttgcca
     3961 cagtttttga catcatctaa ctgactgatt ttcaagatct aaaagtactt atccccagaa
     4021 aaggtcttcc cctatcacta acaatcacta tcttttaaaa tcttttagtt cttttttgag
     4081 aagcggaagg gcaaaccggc ctcgcggcac gccgctcggc cggaacaccc gggtccgttt
     4141 ccgtttgaca cgcccggggg ccttacatag aatggccggc cttttccgac cctctcgggt
     4201 catgttcatt ttttgagcga tcatcatgaa gcgtacattc cagcccagca ccctgaagcg
     4261 cgcccgtacc catggcttcc gcgcccgcat ggctaccaag aacggccgta ccgttctggc
     4321 ccgccgtcgt gccaagggtc gcaagaacct gaccgtctga accgcgcact gttcgatgtg
     4381 actgcgcgct atcgctttcc ccggacgcac cgtctgctgc gtccggcgga attccagacc
     4441 gttttcgata gcacagcatt caaggtcggt gagcagcatt tcctgctgct ggttcgtccc
     4501 aacggtctgg atcgcccccg tctcggtctg gtgatcgcca agaaaaaagt ccggcttgcg
     4561 gttgaacgca accgactgaa gcgtacgatc cgcgacagct tccgcctgca tcaggccgac
     4621 ctgccggccg ttgatgtgat tttcatggcg cgcaacgatc ttggcagcct gctttccgca
     4681 gatctggcca cccagctgga caaaagctgg aagcgcctgg cccgcaaggc aggaacaccc
     4741 gcggcgaccc catccgcaga cgtggcagag cccaaggcat gaaaatcctt ttcattttgc
     4801 cgatacgctt ctaccgcatc gccatcagtc cgatgatggc gccgcattgc cgttttcatc
     4861 cttcctgttc tgcctatgcc gaagaagcca tccaccgtca tggtgcggtg aaaggcatgg
     4921 cgctagcggt caatcgcatc ctgcgttgcc acccttgggc agaaggcgga ctcgatccgg
     4981 ttcctgaaaa attttcgctc cgtcgcgtcg ctccgtcgac cgacattccc gacttacccc
     5041 gcatttccaa ggcgccctga tctatggaac tccgacgcac gttgctcatt gtggccatgg
     5101 cagttgttgg ctacatgctc atcctgacct ggcagaaaga ctatggtgcc aagcctgtcg
     5161 ctgatgcagt ggcggcgact tcccccgcag tggcgccgac agtggccaac ggtgacacac
     5221 cggcagttcc cgccgcaccg gccaacgggg atgtcccggc agtcccctcg gcgcccgcga
     5281 gcacggatgc accggtggag tccaccg
//
LOCUS       DASUEG010000012         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2098013, whole genome shotgun sequence.
ACCESSION   DASUEG010000012 DASUEG010000000
VERSION     DASUEG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098013"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..953)
                     /locus_tag="VFX11_00185"
     CDS             complement(90..953)
                     /locus_tag="VFX11_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937061.1"
                     /translation="MNLSPPRVVLVTGASRGAGKGIALAFGALGDIVYVTGRSQREGD
                     APLPGTVFATAEAITRRGGIGIAVVCDHSDDAQVENLFQKIISEQGRLDILVNNATAL
                     HDDLVRPGPFWEKSVDLVDILDVGLRSSYLASWHAAKIMAAQQRGLIVNTSSPGAACY
                     MHGPAYGAQKAGNDKMVWDMAHDLRPFNVACLSIWMGVLNTERLQLAAAANPEQYAPF
                     LQMAESPEFTGRVIDALYRDPKLMEKSGRALIGAEAGFELGVTDIDGRQPHSHREMLG
                     GPIAFNPAVVQ"
     gene            complement(1188..2390)
                     /locus_tag="VFX11_00190"
     CDS             complement(1188..2390)
                     /locus_tag="VFX11_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HEX4937062.1"
                     /translation="MTAHEKDLQNELSPAALVQRARDMVPYLQETAARAEAERRIPVE
                     TVQKMKDAGFFRVLQPKRWGGYQMDPQVFFDIQLTLAEGCMSTAWIYGVIAVHNWQIA
                     LFDLKAQEDIWRDDTNVLIASSYMPVGKVVPVEGGFKFSGRWAFSSGCDHCDWVFLGG
                     VVPPTAENPAPDYRTFLVPRSDFKVLDTWHTFGLRGTGSNDIVVDDVFVPEYRTHSSL
                     DGFRGTNPGIDSKTVPLYKIPFGQLFPRAVSGSTIGATQGAINAYRDVASKRIGTNTG
                     AKTAEDPHAQMAVARAQALVDQLKLRLNAIFDRLMDDARRGEATDMTQRIQYRYESAA
                     VPEACLAEVLELQKMCGGRGIFTSSPLQRFVLDILAGRAHVANNPYQFGRNYGGVQLG
                     LESTDFFL"
     gene            complement(2443..2940)
                     /locus_tag="VFX11_00195"
     CDS             complement(2443..2940)
                     /locus_tag="VFX11_00195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX4937063.1"
                     /translation="MTKTQMDSRAFRTALGQFATGVTVITALDAAGNRVGMTANSFSS
                     VSLDPMLVLWSIARTSKSYDVFMQAPHFAIHVLNAGQKALSSQFASTCDDRFQGIETR
                     TGEGGVPLLTDYSALFQCATEARYDGGDHVIIIGRVLDFDSTTKPPLIFHAGRYADIE
                     LPVAV"
     gene            complement(3034..3867)
                     /locus_tag="VFX11_00200"
     CDS             complement(3034..3867)
                     /locus_tag="VFX11_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4937064.1"
                     /translation="MLGMEDVSAQLRAPGDSSVFLKMDDRPFRIVVSQSGQDNFGFSG
                     WEIAGPVAFAEAVQTLEKHGVNFERAGADVLAARRVQELVSFADPDGNRHEIYWGPVS
                     DFRQFASPVGIKQFITGDQGFGHVVLPTPAFDKCLPFYRDVMGFGLSDLMKVRFTPDP
                     AEPEKRLYFMHCNERHHSLAIFECPMPSGCVHVMVEVDRVDEVGRAMDRMRANGVKLM
                     GTLGRHANDHMVSFYMATPSGFFIEYGAEGRTVADWSRYSPFQSTVNSFWGHDFSVGH
                     Q"
BASE COUNT          731 a         1115 c         1192 g          829 t
ORIGIN      
        1 catggtttgg ctcctggcgt ccttgaattg tttgttatgg gactctgtta ctgaagactt
       61 gttactgcac cactgttatt gcaccgctgt tattgcacga ctgccggatt gaacgcgatg
      121 gggccgccca gcatttcgcg gtgcgagtgg ggctggcggc cgtcgatgtc ggtaacgccc
      181 agttcgaaac ctgcctcggc gccaatcagc gcgcggccgg atttttccat cagcttcgga
      241 tcgcggtaca gtgcatcgat cacgcggccg gtgaattccg gtgattccgc catttgcaga
      301 aacggtgcgt actgctcagg attggcggcg gcggccagtt gcaggcgttc ggtgttgagc
      361 acgcccatcc agatcgacag gcaagccacg ttgaaagggc gcaggtcgtg cgccatgtcc
      421 cacaccattt tgtcgttgcc ggctttctgg gcgccgtagg caggaccgtg catgtagcag
      481 gctgcgccgg gggagctggt attgacgatc aggccgcgct gctgcgccgc catgattttt
      541 gccgcatgcc agctggccag atagctggag cgcaggccca cgtcgagaat atccaccagg
      601 tcgacggatt tttcccagaa cgggccgggt cgcaccagat cgtcgtgcag tgcggtggcg
      661 ttgttcacca gaatatcaag acggccctgt tcactgatga ttttctggaa caggttttcc
      721 acctgcgcat cgtcgctgtg atcgcatacc acggcaatgc cgatgccacc gcgccgggtg
      781 atggcttcgg cagtggcgaa cacggtaccg ggcaggggcg catcgccttc gcgctggctg
      841 cggccggtga catatacgat atcgcccagt gcgccgaacg ccagtgctat gcctttgccg
      901 gcgccacggc tggcacctgt taccaatacg acgcgcggtg gagataaatt catggttgta
      961 atcccggtta gctagggaac atctgaataa ctactgcgct tgcaaatact gcgttaaaaa
     1021 cgggctcaaa atcctcattt actcaatgta aactgcggct tttcgcccgt ttttgtcttg
     1081 tctttgctgc gctcgctacg ttattcagat gttccctaaa aaatccgcaa ccctgcgctg
     1141 ctgtacgcag gattgcggca agaacgtgtc cgaggacacg agcgcgatca aaggaagaaa
     1201 tcggtacttt ccaggcccag ctgcacgcca ccatagttgc ggccgaactg gtacgggttg
     1261 ttcgccacgt gggcacgccc ggccaggata tccagcacaa aacgctgcag cggactgctg
     1321 gtgaaaatgc cacggccgcc gcacatcttt tgcagttcca gcacttccgc cagacaggct
     1381 tccggcacgg cagcggattc ataacggtac tggatgcgtt gtgtcatgtc ggtggcttcg
     1441 ccgcggcggg catcgtccat cagccgatcg aaaatcgcat tcaggcgcag cttgagctgg
     1501 tccaccagcg cttgtgcgcg tgccaccgcc atctgtgcgt ggggatcttc ggcagtcttg
     1561 gcgccggtgt tggtgccgat ccgctttgat gccacatcgc ggtacgcatt gatcgcaccc
     1621 tgggtggcgc cgatggtgga accggacacg gcgcggggga acagctggcc aaacggaatt
     1681 ttatacagcg gcacggtttt ggaatcgatg ccgggattgg tgccacggaa gccgtccagc
     1741 gaactgtggg tgcggtattc cggcacgaac acatcgtcca ccacgatgtc gttactgccg
     1801 gtgccgcgca ggccgaaggt gtgccaggtg tcgagcacct tgaaatcact gcgtggtacc
     1861 aggaacgtgc ggtagtccgg tgccggattt tctgccgtgg gaggcaccac gccgccgagg
     1921 aacacccagt cgcagtgatc gcagccgctg gaaaatgccc agcgtccgct gaatttgaaa
     1981 ccgccttcca ccggcaccac tttgcccacc ggcatatagg acgatgcaat cagcacgttg
     2041 gtgtcgtcgc gccagatgtc ttcctgcgct ttcagatcga acaatgcgat ctgccagttg
     2101 tgcactgcga tcacgccgta aatccaggcg gtggacatgc agccttccgc cagcgtcaac
     2161 tggatatcga agaacacttg cgggtccatc tggtagccgc cccagcgttt gggctgcagt
     2221 acgcggaaga aaccggcatc cttcattttc tgcaccgttt ctaccggaat gcggcgctcc
     2281 gcttcggcgc gggctgcggt ttcctgcagg tagggcacca tatcacgtgc ccgttgcacc
     2341 aacgcggcgg gggacagttc gttctgcaga tccttttcgt gggctgtcat ggtcatgttc
     2401 tcgctgatgg taatgtgcgt tatcggttct tgttatggct ggtcagactg caacgggcaa
     2461 ttcgatatcg gcgtaacggc ctgcgtggaa aatcaggggc ggtttggtgg tgctgtcaaa
     2521 atccagcacg cggccgatga tgatgacgtg atcgccgccg tcgtagcgcg cttcggtggc
     2581 acactggaac agcgcgctgt aatcggtcag caggggtacg ccaccttcgc cggtgcgggt
     2641 ttcgatgccc tggaagcggt cgtcgcaggt ggaagcgaac tgggatgaca gggctttctg
     2701 cccggcgttc aggacatgga tggcgaagtg gggggcctgc atgaacacgt cataggattt
     2761 ggaagtgcgg gcgatgctcc acagcaccag catgggatcg agggataccg agctgaaact
     2821 gttggcggtc atgcccaccc ggttgccggc cgcgtcgagg gcggtgatga cggtgacacc
     2881 ggtggcgaac tggcccagcg cggtgcgaaa tgcccttgaa tccatttgtg tttttgtcat
     2941 gttgagaccc gttttttcgt tacctttcca tgaccgtgat cttcgcaaag ccagtgtggg
     3001 aattcatacc tcaaacggcc tagtccgttt gggttattgg tgtcccacgc tgaaatcatg
     3061 tccccagaaa ctgttcactg tgctctggaa cggtgagtag cgcgaccagt cggcaaccgt
     3121 gcggccttcc gcgccgtact cgatgaaaaa gcccgacggc gtcgccatgt agaaactcac
     3181 catatgatca ttggcgtggc ggcccagggt gcccatcagc ttcacgccgt tggcgcgcat
     3241 gcggtccatg gcgcggccca cttcatccac gcggtccact tccaccatca cgtgtacgca
     3301 gccggacggc atcgggcatt cgaaaatcgc caggctgtgg tgacgttcgt tgcagtgcat
     3361 gaagtacagg cgtttttccg gttcggcggg atcgggcgtg aagcgcacct tcatcaggtc
     3421 agacaggcca aagcccatca cgtcgcggta gaacggcagg cacttgtcga acgcgggtgt
     3481 gggcagtacc acatggccga agccctggtc gccggtgatg aactgcttga tgccaacggg
     3541 cgaggcgaac tggcgaaaat cggaaaccgg accccagtag atttcgtggc ggttgccatc
     3601 cggatcggca aagctcacca attcctgcac gcggcgcgcc gccagtacgt cggcaccggc
     3661 gcgttcgaaa ttcacgccgt gtttttccag tgtctgtacc gcttcggcaa aggcaacggg
     3721 tccggcaatt tcccagccgc tgaaaccgaa gttgtcctgg ccggattggc tgacgacgat
     3781 gcggaagggg cggtcgtcca ttttcaggaa tacgctgctg tcaccgggtg cgcgcagttg
     3841 cgccgacaca tcctccatgc ccagcac
//
LOCUS       DASUEG010000013         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_375090, whole genome shotgun sequence.
ACCESSION   DASUEG010000013 DASUEG010000000
VERSION     DASUEG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375090"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..715
                     /locus_tag="VFX11_00205"
     CDS             293..715
                     /locus_tag="VFX11_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937065.1"
                     /translation="MDRRNFVTLGAIGSAAGLLLPAALQAADAGSLQTPFAGGIYYTK
                     DKPGRWAAKAAGHSPVVESAGKMVLVTTPHEMKGYEHFIVKHVLLDANMNLLGEKLFN
                     PLQDKAAVSQFEIAAYSGPAYALSMCNQHDVWVTAFTV"
     gene            complement(712..1815)
                     /locus_tag="VFX11_00210"
     CDS             complement(712..1815)
                     /locus_tag="VFX11_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937066.1"
                     /translation="MPSLLKSPVNYVRHAVERRTHTEKFIVADKSAHQTIYRDGLMEL
                     RYYPHQDQAPFLLDGEAVTPLAQQHRVPVLLVPPLGVYHWIYDLMAERSWVRFLNARG
                     FQVYLVNWGAPGFKDAHLSLDTYVNRWMTEAVEQVQQHSGQRDISIVGYCMGGLLTLL
                     YAGAHDQGQLRNIVTIASPIDFHARPGGALLGWAKKGVSLLPLDRFDIRPEKFHVPGD
                     LLSVLFKLTNPFAGVVSYFDLVRNLADRDYVKAHLTTREWFNNMPDYPGATVQQLLVD
                     FTLLNKLATGRVQIGDRESDLTGIKSNLLAFAGKSDRIVSIRAAKRLMELVQSQDRTF
                     KVVPGGHAGVFAGGKAQEHTWAITADWLAQRSD"
     gene            1897..>2348
                     /locus_tag="VFX11_00215"
     CDS             1897..>2348
                     /locus_tag="VFX11_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4937067.1"
                     /translation="MTRNFLRWLLLWPLLVLSALVKADVVALDPSMQGMPLGRFLEYQ
                     VDTGGKASLEQIRARPDWLPLAVDVPNFGLAMPAHWFRLELQVADPGRPWLLEIGYSE
                     VDKLDFYLLDESGRLLDQRELGARRPRAEGSVLHLRPVIPLRLQQPGV"
BASE COUNT          418 a          685 c          766 g          479 t
ORIGIN      
        1 cagctggccg ccaaactgct gggtgacctg gcgggcttcc tgttgcaacg cggctgcatc
       61 cggttggtcg gtggcattgg tcagcgccgg ccagcaggcg ctgaccagca gcagtatcgg
      121 catccatagt ttcatcgcga atctccggtt aaataatcca gcccagtgta cgaaatcctg
      181 ccaccgtcca aattgatcca gatatgctta gttgtgaata agtcgctagt tggtagcacc
      241 cgcctggctt aggcttggcg tcgattgaag accaaccaat aggagatacg ccatggatcg
      301 tcgcaatttt gtcactttgg gagcgattgg cagtgcagca ggattgttgc tgccggccgc
      361 attgcaggcc gccgatgcgg gttccctgca gacacccttc gccgggggca tttattacac
      421 caaggataag ccgggccgct gggccgccaa ggcggcggga cattcaccgg tggtggaaag
      481 cgcgggcaag atggtgctgg tgaccacgcc ccatgaaatg aaaggctacg aacatttcat
      541 cgtgaagcat gtgctgctgg atgcgaacat gaacctgctg ggagaaaagc tgttcaatcc
      601 gttgcaggac aaggcggcgg tgtcgcagtt cgaaattgcc gcttactccg gccccgccta
      661 tgccctcagc atgtgcaacc agcatgatgt gtgggtgaca gcctttacgg tctagtccga
      721 ccgctgcgcc agccagtccg ccgtgatcgc ccaggtgtgt tcctgggcct tgccccccgc
      781 aaatactccc gcgtgcccgc cgggcactac cttgaaggtg cggtcctgtg attgcaccag
      841 ttccatcagc cgttttgccg cgcggatgct gacgatgcga tcgctttttc cggcgaacgc
      901 cagcaggttg ctcttgatgc cggtgagatc ggattcccgg tcgccaatct gcacccgtcc
      961 cgtggccagt ttgttcagca acgtgaaatc caccagcagc tgctgcacgg tggcgccggg
     1021 ataatccggc atgttgttga accattcccg cgtggtgagg tgggccttca cgtaatcccg
     1081 gtccgccagg ttgcgcacca gatcgaaata actgaccacg ccggcgaaag gattggtgag
     1141 cttgaacagc accgacagca ggtcgccggg cacgtggaat ttttccggcc ggatgtcgaa
     1201 gcggtccagc ggcagcagcg acacaccttt tttcgcccag cccagcagtg caccgccggg
     1261 acgggcatga aaatcgatgg ggctggcgat ggtgacgata ttgcgcagct ggccctgatc
     1321 gtgggcgccg gcatagagca gggtgagcag gccgcccatg cagtagccga cgatggagat
     1381 gtcacgctgg ccgctgtgct gctgtacctg ctccacggcc tctgtcatcc agcggttgac
     1441 gtaggtgtcg agggacaggt gggcatcctt gaagcccggt gcaccccagt tcaccagata
     1501 gacctggaag ccgcgtgcgt tcagaaaccg cacccagctg cgttcggcca tcaggtcgta
     1561 gatccagtga tacaccccca gcggcggcac cagcaggacc ggcacccggt gctgttgcgc
     1621 caggggggtg acggcttcgc cgtccagcag gaagggggcc tggtcctggt gcgggtagta
     1681 gcgcagctcc atcaggccgt cgcgatagat cgtctggtgg gcgcttttgt ccgccacgat
     1741 gaatttttca gtgtgggtgc ggcgttccac cgcgtgccgg acgtagttga ccggggactt
     1801 caggagcgaa ggcattgcca tggatgcgga actcgggatg aacgtcttct atagtttaat
     1861 ggaatgattt ccgattgccc tggccgattt tatcccatga cccgaaattt cctgcgctgg
     1921 ctgctgctgt ggcccctgct tgtgctgtcg gccctggtga aggccgatgt ggtggcgctg
     1981 gacccctcga tgcagggcat gccgctgggt cgttttctgg aatatcaggt agatacgggc
     2041 ggcaaggcca gtctggagca gatccgggcc cggccggact ggctgcctct ggcggtggat
     2101 gtgcccaatt tcggcctggc catgccggcg cactggttcc ggctggagct gcaggtggcg
     2161 gaccccggcc gtccctggct gctggagatt ggctacagcg aggtggacaa gctggatttc
     2221 tatctgctgg acgaatccgg ccggctgctg gaccagcgcg agctgggcgc gcgacggccc
     2281 cgcgcggagg gcagcgtgtt gcacctgcgc ccggtgattc cgctgcggct gcagcaaccg
     2341 ggggtgat
//
LOCUS       DASUEG010000014         4977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1214150, whole genome shotgun sequence.
ACCESSION   DASUEG010000014 DASUEG010000000
VERSION     DASUEG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214150"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFX11_00220"
     CDS             complement(<1..449)
                     /locus_tag="VFX11_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937068.1"
                     /translation="MKIFVAVSFHGFGHLAQVAPLVNTLFARHPDWQLVLQCAAGREL
                     LQHWITPDFIHVQVATDPGIPMHNALRVDAAETWRQYRLDNARNQDDVAGLQKLLALH
                     RPDLVLSNIGYRVARAASQLSIPCFHFCSLNWADSFYPYCAGQADAAA"
     gene            637..2415
                     /locus_tag="VFX11_00225"
     CDS             637..2415
                     /locus_tag="VFX11_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010384070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4937069.1"
                     /translation="MKDHVKESAKRNYIGLATTFHDSAIAIVNAAGEVVFAEATERYL
                     QIKSAINVMPDLLTRAVDLINTYCDPDAQLVFAHTWSEQFKHQYRASHQDIVRKGEQV
                     REQMGDVPFHLHREFEFIKSITDFLLNSTEFAGKTLAFELGQVENRRHLPADITRKYD
                     HHLTHAAAACYSSPHDEAVCVVVDGFGEHGASEVFAFKDGQFTSLLPKVEGEGSLGAF
                     YKWICRTCGFGLLSGEEWKVMGLAPYGKLDANLYRILRSTLKVNGLNNLTGGDLQMGR
                     VFGELYRRERRNNEPLLDYADLAFTGQAIFEEVLFELLNNVYDLDVSRNLVMAGGCML
                     NSSANGKVLDNTRFDDLFVFSAPADDGNALGAALLAYYEDHPYRPKAPQQIQSPYLGS
                     RMSHKTLEHVKQFGRIAKHQSLPGRIHRKAAELLAEGKIIAWVQGRAEFGPRALGNRS
                     ILADPRSPAVKERVNGEIKFREEFRPFAPSILHEFGPQYFEHYQETPYMERTLKFRSD
                     VLDRVPGVVHVDHTGRLQTVKREWNERYYDLIKAFHDITGVPLVLNTSFNVMGKPIVH
                     SVEDALAVFFTSGLDALIIEDELFEK"
     gene            2519..2701
                     /locus_tag="VFX11_00230"
     CDS             2519..2701
                     /locus_tag="VFX11_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937070.1"
                     /translation="MSNKALISIAIVLLIGILSIAVIRIQNPPEAESIGDKVSRSINE
                     GAEEAADEIDDHTDDR"
     gene            2892..>4977
                     /locus_tag="VFX11_00235"
     CDS             2892..>4977
                     /locus_tag="VFX11_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4937071.1"
                     /translation="MDSRRLRQVLSRNYILFVILLTSCAAALCYSLLSSKDNVEKTDE
                     LINHTHEIINLAEQMAGLVEGMLASQRGYLLSGDTAFLDKYEKNKAIVSEQLARLNEL
                     TADNKSQQSRLDEVRNYFALFTNHLEERAERFQPQPLPELVEGVAVIDDIRTNIGRIN
                     QAVLAEEHQLLQLRVQALEREKTQYVNTLVAGIVVGTVLLLVFNAFLLNAQAGRSQAE
                     ASLKDSQERFALAVEGAQDGIFDWNMRDNTLYFSAQFFRILGYEKTRNYSLNDFIELL
                     HPDDKDKSWATIAAGDRQCKPEYAQEFRLRHQSGRWIWIQARGKILFDDKLCPYRIVG
                     AHTDISQVVQANEKLQVERNLAQSANQAKSEFLAHMSHEIRTPLTAISGIAEILERQK
                     DTLTERQQKLVHTLALSSSSLKDLISDVLDFSKIESGDIVLENKPFRLGPLFEEIASM
                     MALRASEKGISFLYDCSEVRELSFQGDRSRIRQICVNLISNAIKFTEQGKVSVSAAVE
                     QRDNRAFLRIDVADTGIGISADNFELIFDRFKQVHSSDSRKYGGTGLGLPISKQLATL
                     MGGLITVSSQVGAGSTFSLLLPMQSQVAAMPMTEGPVSAAQLDADIKALITEDTRVLV
                     VEDYAGNIALVSYILDELGIGYDVATTGLMALDLWQKRAYNLVLMDIQMPEMDGFAAT
                     QSIRQIENAHGRV"
BASE COUNT         1158 a         1469 c         1390 g          960 t
ORIGIN      
        1 gccgccgcgt ccgcctgccc ggcacagtag ggataaaacg aatccgccca gttcagcgag
       61 cagaaatgaa aacaggggat gctcagctgc gacgctgccc gcgccacacg gtaaccgata
      121 ttggacagca ccagatccgg ccggtgcaac gccaacaatt tctgcaggcc cgccacatcg
      181 tcctggttgc gcgcgttatc cagccggtac tgacgccagg tttctgccgc atccacccgc
      241 aatgcattgt gcatgggaat acccggatcg gtcgccacct gcacatgaat gaaatccggc
      301 gtgatccagt gctgcagcag ctcgcgaccg gcggcgcact gaaggacaag ctgccagtcc
      361 ggatggcgcg caaacagcgt gttgaccagc ggcgcgacct gggccagatg accaaagccg
      421 tgaaacgaaa ctgcaacaaa aatcttcatg aaggagaacg cctttacagg taaacatgct
      481 gctggtgcgg cccagccttg attgtagaac ctatcagcca aaaaatggat tcgccgctct
      541 gtacaaaata ggtgacactg acgccgggca aggcacacgc cctgccatcg aatggacacg
      601 ccttactcac aatcagaagt ctcgaaaggt gattttgtga aggatcatgt gaaggaatct
      661 gcaaaaagaa attacatcgg actggccacc acatttcatg acagcgcaat cgccatcgtc
      721 aatgcggcag gcgaagtggt gttcgccgag gccacggaac gctacctgca aatcaaatcc
      781 gccatcaatg tgatgcccga tctgctgacc cgcgcggtag acctgatcaa cacctattgt
      841 gatccggatg cgcaactggt gttcgctcac acctggagcg aacaattcaa gcatcaatac
      901 cgggcctccc atcaggatat cgtgcgcaag ggcgaacagg tgcgcgaaca aatgggggat
      961 gttccctttc acctgcaccg cgaattcgaa ttcatcaagt ccatcacgga ttttctgctc
     1021 aacagcaccg aattcgccgg caagaccctg gccttcgaac tgggccaggt ggagaaccgc
     1081 cgtcatctgc cggccgacat cacccgcaag tacgatcacc acctgaccca tgcggcggcc
     1141 gcctgctatt ccagccccca cgatgaagcg gtctgcgtgg tcgtggacgg cttcggcgaa
     1201 cacggcgcca gcgaggtgtt tgccttcaaa gacggccaat tcaccagcct gctgccgaaa
     1261 gtggaaggcg aaggcagcct gggcgcgttc tacaaatgga tctgccgcac ctgcggtttc
     1321 ggcctgctgt cgggcgagga atggaaagtc atgggcctgg cgccttacgg caagctggat
     1381 gccaaccttt accgcatcct gcgctcaacg ctgaaagtga acgggttgaa taacctgacc
     1441 ggcggcgatc tgcagatggg ccgcgtgttc ggcgagctgt atcgacgcga acgccgcaac
     1501 aatgagccgt tgctcgacta cgccgacctg gcctttaccg ggcaagccat cttcgaagaa
     1561 gtcctgtttg aactgctgaa taatgtgtat gacctggatg tgtcacgcaa tctggtgatg
     1621 gccggcggct gcatgctgaa ttcctccgcc aacggcaagg tgctggacaa cacccgtttc
     1681 gacgatctgt tcgtgttcag tgcgccggcg gacgacggca atgcgctggg cgccgcactg
     1741 ctggcctact acgaggatca cccttatcgc cccaaagcgc cgcagcaaat ccagtcgcct
     1801 tacctgggtt cgcgcatgtc gcacaaaacg ctggagcatg tgaaacagtt cggacggatt
     1861 gcgaaacacc agagtctgcc gggccgcatt caccgcaaag ccgccgagct gctggcggaa
     1921 ggcaagatca ttgcctgggt gcagggccgc gccgaattcg gcccccgcgc cctgggcaac
     1981 cgctcgattc tggcagaccc gcgctcgccg gcggtgaagg aaagggtcaa tggcgaaatc
     2041 aaattccgcg aggaattccg tccgttcgcg ccatcgatcc tgcatgaatt cgggccgcag
     2101 tatttcgagc actaccagga aacgccctac atggagcgca ccctgaaatt cagatccgat
     2161 gtgctggacc gggtaccggg cgtggtgcat gtggaccaca ccggccgcct gcagaccgtg
     2221 aagcgggaat ggaacgaacg ttattacgat ctgatcaagg catttcacga catcaccggt
     2281 gtgccgctgg tgctgaacac cagcttcaac gtaatgggca agccgatcgt gcattcggtg
     2341 gaagatgcgc tggcagtgtt tttcaccagc gggctggatg cgctgattat tgaggatgag
     2401 ttgttcgaga aataagaaaa acaccagtgg gaacctttca ctccaggaaa cgtttgtgag
     2461 acagaccgta ttgaacgcgc cggttcacgt tcagcatctg cccacgacgg aggttcccat
     2521 gagtaacaaa gccctgatat ccattgccat cgtgttgttg atcggcattc tgtccatcgc
     2581 cgtcatccgc atccagaatc cgcctgaagc ggaatcgatc ggtgataagg tcagccgttc
     2641 catcaatgag ggcgccgagg aagccgccga cgagatcgac gatcacactg atgaccggta
     2701 accatcagtt gccctccttt ttccggacac cgcgtggggt tgttcgatgc cccactttcc
     2761 aacgctgagc gttgatccgc gcggacgacg cccggctttc tctttcatca catacccacc
     2821 gccgtgacgt gctttacagc gcgtcgcgat gcgcctgata ctggcaattc cgtcacggat
     2881 gagacaagtc aatggattcg cgccgcctgc gtcaggtgtt gtcacgcaac tacatcctgt
     2941 tcgttatcct gctcacttca tgcgcagcgg ccctttgcta ttcgctgctg tccagcaagg
     3001 acaacgtgga gaagacggac gagcttatca atcacacgca cgaaatcatc aatctggcag
     3061 agcaaatggc cggactggtg gaaggtatgc tggcgtcgca gcggggctac ctgctgtccg
     3121 gcgatacagc ctttctggac aaatacgaaa agaacaaggc catcgtgtcg gaacagctgg
     3181 ccaggctgaa cgaacttacc gctgacaaca aatctcaaca aagccggctt gacgaagtcc
     3241 gcaactattt cgcgctgttc accaatcatc tggaagaacg cgccgagcgc tttcagccgc
     3301 aacccctgcc ggaactggtg gaaggcgtcg ctgtgatcga tgacatccgc accaatatcg
     3361 gccggatcaa ccaggcagtt ctggcggagg agcaccagtt gctgcaactg cgggtacagg
     3421 cgctggagcg ggaaaaaacc cagtacgtaa ataccctggt ggccggcatt gtcgttggca
     3481 ccgttctgct gctggtattc aatgcatttc tgctcaatgc acaggcggga cggtcgcagg
     3541 cagaggcctc gctcaaggat tcccaggagc gtttcgcgct ggccgtggaa ggagcgcagg
     3601 acggcatttt cgactggaac atgcgcgaca acaccctgta tttttctgca cagttcttcc
     3661 gtatcctggg ttacgaaaaa acacggaact acagcctgaa cgatttcatt gaactgctgc
     3721 atccggatga caaggataaa agctgggcca ccatcgctgc cggtgatcgc cagtgcaaac
     3781 cggaatatgc ccaggaattc cgcctgcgcc accaatcggg ccgctggatc tggattcagg
     3841 cccgcggcaa gatcctgttc gatgacaagc tgtgcccata ccggatcgtt ggtgcccaca
     3901 ccgacatcag ccaggtggta caggccaacg agaagttaca ggtcgaacga aatctggcgc
     3961 aaagcgccaa ccaggccaaa agcgaattcc tggcccacat gagccacgag atccgcacgc
     4021 cactgaccgc catcagcggc atcgccgaaa ttcttgaacg ccagaaagac acactcaccg
     4081 aacgccagca gaaactggtg cacaccctgg ccttgagctc gtcttccctc aaggatctga
     4141 tcagtgacgt gctggatttc tcaaaaatag aaagcggcga tattgtgctt gaaaacaaac
     4201 cgttcagact cggtccgctt ttcgaggaaa tcgccagcat gatggccttg agggccagcg
     4261 aaaaaggcat cagctttttg tacgattgca gcgaagtccg cgagctttca tttcagggcg
     4321 accgctcccg catccggcag atctgtgtca atctgatcag caacgcgatc aagttcaccg
     4381 aacagggcaa ggtcagcgtc tcggccgcag tggagcagcg cgacaaccgg gcttttctgc
     4441 gaatcgatgt cgccgacacc ggtatcggca tcagcgcgga caacttcgaa ctgatcttcg
     4501 atcggttcaa acaggtgcat tcctcggatt cgcgcaaata tggcggcacc ggcctgggtc
     4561 tgcccatttc gaaacaactg gccaccctga tgggcggcct tattaccgtc agcagccagg
     4621 tgggggccgg ctccactttt tcactgctgt tgcccatgca gtcccaggtt gctgcgatgc
     4681 cgatgaccga aggaccggta tccgccgcgc aactggacgc ggatatcaag gcgctcatta
     4741 ctgaagacac ccgcgtgctg gtggtggagg actacgcagg caatattgca ctggtgagct
     4801 atatcctgga cgaactcggc atcggctacg acgttgccac cacgggactg atggcgctgg
     4861 acctgtggca aaaacgtgcc tacaacctgg tgctgatgga tattcagatg ccggaaatgg
     4921 acggttttgc cgccacccaa agtatccgcc agatcgaaaa cgcccacggc cgcgtcc
//
LOCUS       DASUEG010000015         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2164239, whole genome shotgun sequence.
ACCESSION   DASUEG010000015 DASUEG010000000
VERSION     DASUEG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164239"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1899)
                     /locus_tag="VFX11_00240"
     CDS             complement(7..1899)
                     /locus_tag="VFX11_00240"
                     /inference="COORDINATES: protein motif:HMM:NF011314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX4937072.1"
                     /translation="MSSSADSTLQSVLDALAAVRAELVSPESVPDALLQPVHGDWHES
                     ARNLLHYLALRRQDLRPLQLQLAALGLSSLGRAESCVLPTVDAVLQVLRRLAQQAVVE
                     EEPASPLDFAMGHQMLARHTDNLLGPAPGGRSVRIMVTMPSEAASEYSLVHDLLKQGM
                     NVMRINCAHDDATAWAAMIDNLRRAEATLGLSCRVAMDLAGPKLRTGPLEPGPRVLRI
                     RPERDLYGRVTRPARVWLTQIEAPCSPPAPADATIALPAHWLARLRTGDGIRLQDARA
                     ARRQWQVVDVTATGCWATLEKTTYLIPGIALRHVSVAAPDPAVPADARVVRIPDLPAR
                     ENHLELAAGDLLILTRNQNPGRAAILDSAGNVLTPATIGCSLPSVFNRVQAGETIVFD
                     DGKIGGLIEMVEAERVLVRITRTRPGGARLRADKGINLPDSDLDLPALTAKDCADLAF
                     VAQHADLVQLSFANHAHDVAALLHQLEQQSSRRPAVVLKIETRRGFENLPDMLLTAMR
                     WPTCGVMIARGDLAVECGFERLAEVQEEILWICEAAHVPVIWATQVLETLAREGLPSR
                     AEITDAAMGHRAECVMLNKGPHMLSAVRVLDDILCRMQSHQAKKSSMLRELQLAHTQR
                     MESAEY"
     gene            complement(2036..2410)
                     /locus_tag="VFX11_00245"
     CDS             complement(2036..2410)
                     /locus_tag="VFX11_00245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4937073.1"
                     /translation="MKISGQIEFTITEQTPDAVTSVMPVQPGILNPFGTVHAGATLWF
                     ADVTATALVLAGVKAEENMQGFPLAINLNANLAGNSKSGTFIAKAVYVKKGRSVSIVR
                     TTVTTEDGKLIADVTTSHVPSR"
     gene            complement(2490..2960)
                     /locus_tag="VFX11_00250"
     CDS             complement(2490..2960)
                     /locus_tag="VFX11_00250"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEX4937074.1"
                     /translation="MTLPSGSQSHLDHVRHKLRSQVPLIAHMGVDVISWDGCTVVVEA
                     PLAPNLNTHGTAFGGSLYNVAAMTGWSAVHLTLMDAGHTPSVWVVKGEIEYKTPVRGV
                     LRGSATLSDEDRAHLIAGYENKGRVKVAIDVLIREGEQDAVLFKGVYAAMVADP"
     gene            complement(2976..>3994)
                     /locus_tag="VFX11_00255"
     CDS             complement(2976..>3994)
                     /locus_tag="VFX11_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX4937075.1"
                     /translation="LKKESADFDLELAFNQVKALYKKDIASVHGTGDSLAAAMNAVDA
                     ALERKHTLLLRPLEIVMNDPHIFSGWLSMNKAHVKLDGDKVVWRRNPVYVLDDTYHYL
                     NMDFRADLPAAESIWSHDNDILQKGLKFYRELETRLGESDWLKLSALLDAAQAPAGFS
                     ADDWTQIQSAHYGFQCGMEVMSVLPKLARDAHFYELKVNPDLTVHIPQMLFDDALQEQ
                     MMKVLVPPPATKADEIVAVCGGMFYGREAPSLPPFVSEGAHVEAGQTIYIIEVMKMFN
                     KIQAPFACTIDKVLLENVDGAIVKQGQPLFKVTPDEKFVDVDPAVVAAQRKARTDHLL
                     QGLI"
BASE COUNT          785 a         1315 c         1131 g          763 t
ORIGIN      
        1 cgcgcctcaa tactccgccg actccatcct ctgcgtatgc gcgagctgca gttcccgcaa
       61 catcgaactt ttcttcgcct gatgtgactg catgcgacac agaatatcgt ccagcacccg
      121 caccgcgctc agcatgtgcg ggcccttgtt gagcatcacg cactcggcac gatgccccat
      181 ggcagcatcg gtgatttccg cccgtgacgg caagccttcc cgcgccagcg tctccagcac
      241 ctgggtggcc cagatcaccg gcacgtgggc ggcttcgcag atccagagaa tttcctcctg
      301 cacttccgcc agccgctcga aaccgcattc cactgccaga tcgccgcgcg caatcattac
      361 gccgcaggtg ggccagcgca tggctgtgag cagcatgtcc ggcaaatttt caaatccgcg
      421 ccgggtttcg attttcagca caacggcagg gcgccggctg ctctgctgtt ccaactggtg
      481 cagcagcgcc gccacatcgt gggcgtgatt ggcgaacgac agctgcacca gatccgcatg
      541 ctgcgccacg aacgccagat cggcgcagtc cttggctgtt agcgcaggaa ggtcaagatc
      601 gctgtccggc aggttgatac ctttgtccgc gcgcaaacgt gcaccgcccg ggcgggtgcg
      661 ggtgatgcgc accagcacgc gctccgcttc caccatttca atcaggccgc caatcttgcc
      721 gtcatcaaac acaatggtct cgccggcctg cacacgattg aatacggagg gcaacgagca
      781 gccgatagtc gccggcgtca gcacattgcc ggccgaatcc aggatggcag ctcgccccgg
      841 attctgatta cgggtaagga tcagcagatc accggccgcc agctccaggt gattttcccg
      901 ggccggcaga tccggtatcc gcacgacgcg ggcatccgct ggcacagcag ggtccggagc
      961 agcaacggaa acatgtcgca gcgcaatgcc gggaatcaga tacgtggttt tttccagcgt
     1021 cgcccagcag cctgtcgccg tgacatccac cacctgccac tggcggcggg cagcgcgcgc
     1081 atcctgcagc cggatgccgt cgccggtgcg caggcgtgcc agccagtgcg cgggcaatgc
     1141 gatcgtcgca tcggcgggtg caggcggtga acagggggcc tcaatttgcg tcaaccacac
     1201 ccgcgccggg cgtgtcaccc ggccatacag atcccgttcg ggacggatgc gcagcacacg
     1261 cggacctggc tccagcggcc cggtgcgcag cttcggcccc gccaagtcca ttgccacgcg
     1321 gcaggacaat cccagcgtgg cctcggcccg ccgcaggttg tcgatcatgg ccgcccacgc
     1381 ggtcgcatca tcgtgggcgc agttgatgcg catgacattc atgccctgct tcagcagatc
     1441 gtgcaccagg ctgtattcgc tggcggcttc actgggcatc gtcaccatga tacgcaccga
     1501 tcgccccccc ggcgccgggc ccagcagatt atccgtatgg cgcgccagca tctggtggcc
     1561 catggcaaaa tccagcggcg aagccggttc ttcctccacg accgcctgct gcgccagacg
     1621 ccgcaatacc tgtagcaccg catcgacggt tggcaacacg caagattccg ctcgccccag
     1681 cgacgacaat cccagcgccg ccagctgcaa ttgcagtggt cgcaaatcct gccggcgcag
     1741 ggccagataa tgcagcaggt tgcgcgcact ttcgtgccag tcgccatgca ccggttgcaa
     1801 caacgcatca ggcaccgatt caggcgacac cagttccgcc cgcacggcag cgagggcgtc
     1861 aaggacggat tgcaaggtgg aatcagcgga agaggacacg gcgttgtact cctgtgcgtt
     1921 cagcgcgtcc ggcaggctcg ccaggcgcaa acgcagaata ctggtgatgg cagacctgac
     1981 atcgtattac gacgccgtga aacttgtgtg tcaccggcgg acaaggaacg tttgctcagc
     2041 gcgaaggaac atgactggtg gtgacatcgg cgatcagctt gccgtcctcc gtcgtcaccg
     2101 tggtgcgcac gatactgacc gaacgcccct tcttcacgta cacggctttg gcgatgaagg
     2161 taccgctctt gctgttaccc gccagattgg cattcaggtt gatcgccagc ggaaagccct
     2221 gcatgttttc ctcggccttt acgcccgcca gtaccagcgc ggtagcggtc acatcggcaa
     2281 accagagcgt ggcgccggca tgcacggtgc caaacggatt gagaatgccc ggctgcaccg
     2341 gcatgaccga ggtcacggca tccggagttt gttcagtaat ggtgaattcg atctggccac
     2401 tgattttcat gagcctagcc ctgatatgaa agtcccgaga gagcagtccc gaaagaaaag
     2461 tcctgcacca acaaggaaca gagtatacct tacggatctg ccaccatcgc cgcgtacaca
     2521 cccttgaaca gcacagcgtc ctgctcccct tcgcggatca gcacatcgat cgccactttc
     2581 acccggccct tgttttcata gccggcaatc agatgggcgc ggtcctcatc gctcaacgtc
     2641 gcactaccac gcaatacacc gcgcaccggg gttttgtatt cgatttcacc cttcaccacc
     2701 cacacactcg gcgtatggcc ggcatccatc agggtgaggt gaacggcgct ccagccggtc
     2761 atggctgcaa cgttatacag actgccgccg aatgcggtgc cgtgggtatt gaggttgggc
     2821 gccagcgggg cttccaccac caccgtgcac ccgtcccaag agatgacatc cacccccatg
     2881 tgcgcaatca gtggaacctg actgcgcagc ttgtggcgga catgatccag atgcgattgc
     2941 gatccggacg gaagggtcat gacaaaaaaa tcacgtcaga tcaaaccctg cagcagatgg
     3001 tcggtacgcg ccttgcgttg cgccgccacc accgccggat ctacgtcgac gaatttctcg
     3061 tccggcgtca ccttgaacag gggctgaccc tgcttgacga tggcgccgtc cacgttctcc
     3121 agcagcacct tgtcgatggt gcaggcgaac ggggcctgga tcttgttgaa catcttcatc
     3181 acttcgatga tgtagatggt ctgacccgct tccacgtgcg caccttcgct cacgaacggc
     3241 ggcaggctcg gtgcttcgcg accgtagaac ataccaccgc acaccgccac gatttcatcg
     3301 gccttggtgg ccggcggcgg caccagcact ttcatcatct gctcctgcag tgcgtcatca
     3361 aacaacatct gcggaatgtg cacggtcagg tccggattca ctttcagttc gtagaaatgc
     3421 gcgtcgcgtg ccagcttcgg cagcacactc atcacttcca taccgcactg gaaaccgtag
     3481 tgggctgact gtatctgggt ccagtcgtcg gcactgaaac cggccggcgc ctgcgccgca
     3541 tccagcaacg cactgagttt caaccagtcg ctttcaccca gacgggtttc cagctcgcga
     3601 tagaacttca ggcccttttg caggatgtcg ttgtcatgac tccagatgct ttccgctgcc
     3661 ggcagatcgg cacggaaatc catgttcagg tagtgatagg tgtcgtccag cacatatacc
     3721 ggattgcggc gccagaccac cttgtcgccg tccagcttca cgtgagcctt gttcatgctc
     3781 agccagccac tgaagatgtg cggatcgttc atgacgattt ccagcggacg cagcagcagg
     3841 gtgtgtttgc gttccagcgc cgcatccacc gcgttcatgg ccgccgccag cgaatcgcca
     3901 gtgccgtgca ctgacgcaat atcttttttg tacagcgctt ttacctggtt gaaggccagc
     3961 tccagatcga aatccgcgga ttccttcttc agca
//
LOCUS       DASUEG010000016         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1920926, whole genome shotgun sequence.
ACCESSION   DASUEG010000016 DASUEG010000000
VERSION     DASUEG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920926"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFX11_00260"
     CDS             complement(<1..621)
                     /locus_tag="VFX11_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4937076.1"
                     /translation="MLNINTNISALNAQRNLNKNQATLGTTLQRLSSGLRINGAKDDA
                     AGLAIANRMTTQISATSQAIRNANDGISLVQTAESALGEITGNLQRIRDLSLQASSDT
                     LTQDDRKSLQAEVDQLINEINKVNDTTTFGGRKLFQRAGESDYADQNRSAVIKGLGYY
                     WLSEAESRITTYYGIKSDNNIMRVNLDDSDGAGGTLASVSGYVGADG"
     gene            complement(709..2022)
                     /locus_tag="VFX11_00265"
     CDS             complement(709..2022)
                     /locus_tag="VFX11_00265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4937077.1"
                     /translation="MATLLRCWSVPANFAALYRQDQSTFHLINGLRFFSFAWILAFHT
                     VLVYALKVDKQQFFTLAEQAPLALWWVWNADKAVDLFFVVSGFLIGLILLREIHRTGD
                     VDLPRFYFRRYLRLTPLYVLIALLYWWGGGSNADMLWANLLYINNFLPVEDMAMRWTW
                     TLAVEEQFYLALPLALLWLGRAQARHFWRWMTGLLLMSFAIRALVVVWFPELRLHSMQ
                     DLLAAPGVSDIYYEKLYDNLLTRYGPFVLGVMTAWLYLHRRPALAAWFIQHTGLWRIL
                     DTSALLLFLGLLFYPVNRADHGAPGGLLQLYLVTHHNLFALTISWFMLRTFINLQHPG
                     LLARFLSWRGWQPFSQLTYSMYLTHLMIIGLALANVKAHLQALTSLPSGTLVLYTLML
                     GFALSLLLSILVGVLCWLLVEKPFLNLRDWISEAGSTARATPSPA"
     gene            complement(2171..3748)
                     /locus_tag="VFX11_00270"
     CDS             complement(2171..3748)
                     /locus_tag="VFX11_00270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937078.1"
                     /translation="MRNLAFLLIALLLAGHACADTVEISDTREYFLKIRDALFTTSEP
                     VNAQLAVPEPAQLLPLTELPRAQTEYWVRFSLVNHTRETRWTLDTGNIIADQLVLHAQ
                     TGNRHQIARQGFQKLWPFDLRYGLPLELPSGSVTHLWVYIHSNVGASTPVFAILPQVE
                     YQQKAFSYNTQLLIAISALVILSVYQLLIFIPTRDWAWFWSAMFQLSAALAWSAQAKL
                     LLYGLSAGPSVALLYLPLFIAAATGLMFSRTYLSLFHPQPLARLLDGMAVLAVAAGIG
                     GLLLPAHYYPLLLQWMLGLSVSVMLGCAIWRTWTGFAANRFFLAGTALLILCGFFYLL
                     NQILHLQIIENDILLATRCQVLAMFAFMMGLIERVNLLQRQRPQQDTRTATDPVTRMP
                     NRSAFERDVRAWEAYCKEGIMKDFYLTFFDLVSLRQTNATRGMREGDRLLTLVGTWLQ
                     QQTGNHNVYRIGGDEFVVLTQKSFTWDLTPLYQRLQQEGFRDNQINIGCACYSESSGR
                     SSMLKLADDRLHGIDQP"
     gene            complement(3741..>4627)
                     /locus_tag="VFX11_00275"
     CDS             complement(3741..>4627)
                     /locus_tag="VFX11_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4937079.1"
                     /translation="VLTGGMLISSLLGNLLMIITGQQAATARMVDQRTLELQQQKNEL
                     EGIRNELLQANQHKNLFLANMSHELRTPLNAIIGFSRRLLKSAPDRLNERELEGLHAA
                     LRNGKMLLNLINDLLDLSRIEAGKLEIRMAEVPVQELLTNLCQNLEDDCADKGLTLIT
                     RFHQRPLVLQTDPVRLTQILSNLLVNAIKYTDHGSVTVELVKLHEKNREYCRINIIDT
                     GNGIPDDIRNSLFVAYTRAKEVQLKTIEGSGLGLSIAALLTARLGGTIGVESSPGQGS
                     TFHVQFPLQPTPPDPDDA"
BASE COUNT          949 a         1278 c         1490 g          910 t
ORIGIN      
        1 gccgtccgca ccgacatatc cgctgacgga ggccagggtg ccgcccgcgc cgtcgctgtc
       61 atccaggttg acgcgcatga tgttgttgtc cgacttgatg ccgtaatagg tggtgatccg
      121 gctttctgcc tcggacagcc agtaatagcc aagtcccttg atcacggcgc tgcgattctg
      181 gtcggcgtaa tctgattcgc cggcacgctg gaacagcttg cggccaccga acgtggtggt
      241 gtcgttgacc ttgttgattt cattgatcag ttgatccact tccgcctgca gcgatttgcg
      301 gtcgtcctgt gtcagtgtgt cggaggatgc ctgcagggac aggtcgcgga tgcgctggag
      361 gttgccagtt atttctccga gggcgctttc ggctgtctgc accagggaga tgccgtcgtt
      421 ggcgttgcgg attgcctggc tggtggcgct gatctgggtg gtcatgcggt tggcaatggc
      481 aaggccggcg gcatcgtcct tggccccatt gatgcgcagg ccggaggaca gcctctgcag
      541 agtcgtgccc agcgtggcct gattcttgtt cagattgcgt tgtgcgttca gagccgaaat
      601 attggtgttg atattcagca tggaacagcc ttgctcttga aaaaacaatc cagttacgtc
      661 tggaatcggc cgttctcgat gattctttag gcccccggtc aaaaaaaatc aggccgggct
      721 gggcgtggcg cgcgcggtgc ttcccgcttc ggaaatccag tcgcgcagat tcagaaaagg
      781 cttttccacc agcaaccagc acagcacgcc gaccaggatc gaaagcagca gtgacagcgc
      841 gaatcccagc atcagggtgt acaggaccag tgtgccggag ggtagcgacg tcagcgcctg
      901 caggtgggcc ttcacatttg ccagcgccag cccgatgatc atcaggtggg tcaggtacat
      961 ggaataggtc agctggctga agggctgcca gccgcgccag ctgaggaaac gcgccagcag
     1021 gccggggtgc tgaaggttga taaaggtccg cagcatgaac cagctgatgg tcagcgcgaa
     1081 caggttgtgg tgagtgacca gataaagctg aaggagcccg ccaggtgcac catggtcggc
     1141 ccggttgacc ggatagaaca gcaggccgag aaacagcagc aaggcgctgg tatccagtat
     1201 gcgccacaga ccggtgtgct ggatgaacca ggccgccagg gcagggcgcc ggtgcaggta
     1261 cagccaggcc gtcatcacac ccagtacgaa tggtccgtaa cgggttagca gattgtcgta
     1321 gagcttttca tagtagatgt cggagacgcc gggagctgcc agcagatcct gcatggaatg
     1381 cagacgcaat tcgggaaacc agacaaccac cagcgcgcgg atcgcgaaag acatcagtag
     1441 cagacctgtc atccagcgcc agaaatgccg cgcctgtgcc cgtcccagcc acagcagggc
     1501 cagcggcagc gccagataga actgttcttc caccgccagc gtccaggtcc agcgcatggc
     1561 catgtcttcc accggcagga agttgttgat gtacagcaga ttggcccaga gcatgtcggc
     1621 attactgccg ccaccccacc agtacagcag ggcaatcagc acatagaggg gggtcaggcg
     1681 caggtagcgg cggaaataga agcgcggcag gtcgacatca cctgtcctgt ggatttcccg
     1741 taacaggatc agcccgatca gaaagccgct gacgacgaaa aacagatcca ccgccttgtc
     1801 ggcattccac acccaccaga gtgccagtgg cgcctgctcg gccagggtga agaactgttg
     1861 cttgtcgacc ttgagtgcgt aaaccagcac cgtatggaag gccagtatcc aggcaaagct
     1921 gaaaaagcgc aggccgttaa tcagatggaa cgtcgactga tcctgacggt acaacgcggc
     1981 aaaattcgcg ggcactgacc agcaacgcag gagggttgcc atcctggctg gcagccgggc
     2041 tgttaccacg ggcatggtcg tcctcgatct tatttatttt tgtgtgagac aaaatagtaa
     2101 aatagtatca gatcgggctc aaggccattc tggtactggt ttgtaacccc ggattggcgg
     2161 cgtcggtcaa tcagggctga tcgatgccgt ggaggcggtc gtccgccagt ttcagcatgc
     2221 tggaacggcc gctggattcg gagtagcagg cgcagccgat gttgatctgg ttgtcgcgga
     2281 agccttcctg ttgcagacgc tggtagaggg gggtcagatc ccaggtaaag gatttctggg
     2341 tcagcaccac gaattcgtcg ccgccgatgc gatacacatt gtgattgcca gtctgctgct
     2401 gcagccaggt gcccaccagg gtcagcaggc ggtcgccttc acgcatgccg cgggtggcat
     2461 tggtctggcg caggctcacc agatcaaaga aggtgagata gaaatccttc atgatgcctt
     2521 ccttgcagta ggcttcccag gcccgtacgt cgcgctcgaa ggcgctgcgg ttgggcatgc
     2581 gggtgacggg atcggtggcg gtgcgggtgt cctgttgcgg acgctgccgc tgcagcaggt
     2641 tcacgcgctc gatcagcccc atcatgaagg cgaacatcgc cagcacctgg caacgggtcg
     2701 ccagcaggat atcgttttcg atgatctgca agtgcagtat ctggttcagc aggtaaaaga
     2761 atccgcacag aatcagcagg gctgtgccgg ccaggaaaaa acggttggcg gcaaagccgg
     2821 tccaggtgcg ccagatggca cagcccagca tgaccgacac actcaggccc agcatccatt
     2881 gcaacagcag tgggtagtaa tgggccggca gcagcaggcc gccgatgccg gcggccacgg
     2941 ccagcacggc catcccgtcc agcaggcgcg ccaggggctg gggatgaaac aggctcagat
     3001 aggtgcggct gaacatcagc cctgtagcgg cggcgatgaa gaggggcaga tagagcagcg
     3061 ccaccgatgg cccggccgac agtccgtaga gcagcagttt tgcctgcgcc gaccaggcaa
     3121 gcgcggctga cagctggaac attgccgacc agaaccaggc ccagtcgcgg gtgggaatga
     3181 aaatcagcag ttggtacacc gacaatatca ccagcgcact gatggcaatc agcaactggg
     3241 tgttgtagct gaaggctttc tgctggtatt ccacctgcgg caggatcgcg aagaccggcg
     3301 tcgacgcgcc cacgttgcta tgaatgtaaa cccacagatg ggtgacgctg ccggagggca
     3361 attccagggg caggccatag cgcaggtcga atggccagag cttctgaaaa ccctgccggg
     3421 caatctggtg acggttaccg gtctgcgcgt gcaataccag ctgatcggca atgatgttgc
     3481 cagtgtcgag ggtccagcgg gtttcccgcg tatggttgac gagactgaag cgcacccagt
     3541 attcagtttg tgcccgtggc agctcggtca gcggcagcag ttgtgccggt tcgggtacgg
     3601 ccagctgcgc gttgacgggt tcgctggtgg tgaacagggc gtcgcggatt ttcaggaaat
     3661 attcgcgtgt gtcggagatt tccaccgtgt cggcacacgc atggcccgcc agcaacaggg
     3721 caatcagcag gaaggccagg ttacgcatcg tccgggtccg gtggggtggg ctgcagggga
     3781 aactggacat ggaatgtgct gccttgtccg gggctggatt cgaccccgat ggtaccgccc
     3841 aggcgggccg tcagcaatgc cgcgatggac aaccccagcc cgctgccttc gatggttttc
     3901 agctgcactt ccttcgcccg tgtgtaagcg acgaacaggg agttgcggat gtcatccgga
     3961 atgccgttgc cggtatcgat gatgttgatg cggcagtact cgcggttctt ttcatgcagt
     4021 ttcaccagct ccacggtgac gctgccgtgg tcggtgtact tgatggcatt gaccagcagg
     4081 ttggacagaa tctgggtgag gcgcaccgga tcggtctgca aaaccagcgg ccgctggtgg
     4141 aagcgagtga tcagggtgag gcccttgtcg gcgcaatcat cctccagatt ctggcagaga
     4201 ttggtcagca gctcctgaac cggtacctcc gccatccgga tttccagctt gccggcttca
     4261 attcgcgaaa ggtccagcag atcgttgatc aggttcagca acattttgcc gttgcggagc
     4321 gccgcatgca gcccttcgag ctcgcgctcg ttgagacggt ccggggcgga tttcagcagg
     4381 cgccggctga aaccgatgat ggcattgagc ggcgtgcgca gttcgtgaga catgttggcc
     4441 agaaacaggt tcttgtgctg gttggcctgc agcagttcgt tgcggatgcc ttccagttcg
     4501 tttttctgct gctgcagttc cagtgtgcgc tgatccacca tgcgcgcggt ggcggcctgt
     4561 tggccggtaa tgatcatcaa cagattgcct agcaggctgc tgatcagcat gccgccagtg
     4621 agcacta
//
LOCUS       DASUEG010000017         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1015144, whole genome shotgun sequence.
ACCESSION   DASUEG010000017 DASUEG010000000
VERSION     DASUEG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015144"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFX11_00280"
     CDS             <1..971
                     /locus_tag="VFX11_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4937080.1"
                     /translation="IAMAWRAGCRVGNMEFNQFHPTCLYHPQAGNFLITEAMRGEGGH
                     LKLPDGTRFMQRFDERLELAPRDIVARAIDHEIKRLGIDCVYLDISHKPADFIKAHFP
                     TIYERCLEFGIDITQEPIPVVPAAHFTCGGVLTDLNGCTDVEGLYAIGETAFTGLHGA
                     NRLASNSLLECMVFAIAASKDIRARFNDPLQPVTIPDWDESQVTDSDEDVVISHNWDE
                     LRRFMWDYVGIVRTDKRLQRASHRVLLLQGEIREYYSNYKISNDLIELRNLVQVAELI
                     INCALARKESRGLHYTLDYPDLLPEAKDTLLVPKHYVPRAVPAVSG"
     gene            complement(963..1415)
                     /locus_tag="VFX11_00285"
     CDS             complement(963..1415)
                     /locus_tag="VFX11_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX4937081.1"
                     /translation="MWTPDLRLQASPGLSALILALHGLAALALVQMGGGWLWWIPLVL
                     ISGILHLFQTGLKRLPGSVQRVWLGPLGWHLQRRNGQQRGPFLLGSASRIDTRFIRLS
                     LHHQGGMLARLWPAHHLILTPAMIGNDDFRRLQVFLRWSPEKNQAPLS"
     gene            complement(1402..1668)
                     /locus_tag="VFX11_00290"
     CDS             complement(1402..1668)
                     /locus_tag="VFX11_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX4937082.1"
                     /translation="MSESNPSQSEHYRRLQWQCRRGIKEVEVLLVPFFERHYLALSPA
                     DQALFEKLLDQHDVEMFEWFTHRSLPEAPDLARIVKMVLHGVDA"
     gene            1818..>2672
                     /locus_tag="VFX11_00295"
     CDS             1818..>2672
                     /locus_tag="VFX11_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937083.1"
                     /translation="MWHTAYSFQQHPRWGLHARPLEQEAADLLAGASLAACLSTLEII
                     RVTGTDALTFLQGQTTCDFREVSEGHPRLGAHCNAKGRVQASFRAFHWDGDFLLLVPR
                     GQGEPTRAALAKFALFSKASLSLDTTLAPLALLGPQAADWAKALFGAAPAPGNSIANE
                     NGLITALDADRFLTLVPSERCVAVIKNLQALNAALAGDNVWWLSEIRAGIAQIPLEQS
                     EQWIPQEINYDLVNGVNFKKGCYKGQEIVARIHYRGQTKVRVFPVQIGPVQIEPVQND
                     TTVVPGTRI"
BASE COUNT          534 a          821 c          824 g          493 t
ORIGIN      
        1 gcattgccat ggcctggcgc gccggttgcc gggtgggcaa catggagttc aaccagtttc
       61 accccacctg cctgtatcac ccgcaggcag gcaacttcct gatcaccgag gccatgcgcg
      121 gcgaaggcgg ccatctgaaa ctgccggacg gtacccgctt catgcagcgt tttgatgagc
      181 ggctggaact ggccccccgg gacatcgtgg cccgcgccat tgaccatgaa atcaaacgac
      241 tgggtatcga ctgcgtgtac ctggatatca gccacaagcc agccgacttc atcaaggccc
      301 acttccccac catctacgag cgctgtctgg aattcggcat tgatatcacc caagaaccga
      361 ttccagtggt acccgcagcc cacttcacct gcggtggcgt gctgacggac ctgaacggct
      421 gcaccgatgt ggaagggctg tatgccatcg gcgagaccgc gttcaccggc ctgcacggcg
      481 ccaaccgtct ggccagcaat tcattgctgg agtgcatggt gtttgccatt gccgccagca
      541 aggacatacg cgcccgcttc aatgatcccc tgcaaccagt cacgatcccc gactgggatg
      601 aaagccaggt gacggattcc gacgaggacg tggtgatctc ccacaactgg gacgagctgc
      661 gccgcttcat gtgggactat gtgggcatcg tgcgcaccga caagcggttg cagcgggcca
      721 gccaccgggt gctgctgctg cagggtgaga ttcgcgagta ctacagcaac tacaagatca
      781 gcaacgacct gatcgaactg cgcaacctgg tgcaggtggc cgagctgatc atcaactgcg
      841 ctctggcacg caaggaaagc cgcggcctgc attacacgct ggattacccc gatctgctgc
      901 cggaggcgaa ggatacgttg ctggtgccaa agcactatgt gccgcgggcg gtgcccgcag
      961 tgtcaggata gtggcgcctg attcttttcc ggcgaccagc gcaggaaaac ctgcaggcgg
     1021 cggaaatcgt cattgccgat catggcgggt gtgaggatca ggtggtgggc aggccacagg
     1081 cgggccagca tgccaccttg gtgatgcagg ctgaggcgga tgaaccgggt gtcgatgcgg
     1141 ctggcagatc cgagcaggaa tggcccccgc tgctgtccgt tacggcgttg cagatgccag
     1201 cccagtggcc ccagccagac cctttgcacc gatcccggca agcgcttcag gcctgtctga
     1261 aacaggtgca gtattccgct gatcagcacc agagggatcc accacagcca gccgccaccc
     1321 atctgcacca gcgccaatgc agccagcccg tgcagggcaa ggatcaacgc tgacaggccc
     1381 gggctcgcct gcaggcgcag gtcaggcgtc cacaccgtgc agcaccattt ttacgatccg
     1441 cgccagatcc ggcgcctcgg gcagcgagcg gtgggtgaac cactcaaaca tctccacatc
     1501 atgctggtcc agcagctttt caaacagggc ttgatcggcg ggtgacaggg ccaggtaatg
     1561 ccgctcgaaa aacggcacca gcagtacttc cacttccttg atgccccggc ggcattgcca
     1621 ctgcaggcgg cggtagtgtt cggattggga cgggttggat tcggacattg tcggactgac
     1681 tcccctggac aggcctcgca ttataggaaa tgcgccgcgc tgcgcctatg gacccgtgtc
     1741 agtaacaggc tggtatttcg attgcagaac cgtatgatgg ttttcatttg cattcatctg
     1801 tccgggattt tcgcaccatg tggcacacgg cttattcctt ccaacaacat ccccgctggg
     1861 gcctgcacgc gcgcccgctt gagcaggagg cggctgacct gctggccggc gcttcgctgg
     1921 cggcctgtct cagcactctg gaaatcatcc gcgtcaccgg caccgatgcc ctgaccttcc
     1981 tgcagggcca gaccacctgc gattttcgcg aggtaagcga gggccatccc cggctgggcg
     2041 cccactgcaa tgccaagggg cgggtgcagg cgagtttccg cgcctttcat tgggacggcg
     2101 atttcctgtt gctggtgccg agaggccagg gtgaacccac ccgggcggcg ctggcgaaat
     2161 tcgcgctgtt ttccaaggcc agcctgagcc tggataccac gctggcgccg ctggcactgc
     2221 tgggaccgca ggcagcggac tgggctaaag ccctgtttgg tgcagcgccc gcgcctggca
     2281 acagcattgc gaacgagaac ggcctgatca cagcactgga cgctgaccgt ttcctgacgc
     2341 tggtgccgtc ggagcgctgc gtggcggtga taaaaaacct gcaggcactg aacgccgcgc
     2401 tggcgggaga caatgtctgg tggctgtcgg aaatacgggc gggcatcgcg cagattccgc
     2461 tggaacagag cgaacaatgg attccgcagg agatcaatta cgatctcgtc aacggtgtga
     2521 atttcaagaa gggctgttac aagggccagg aaatcgtggc gcggattcat tatcgcggcc
     2581 agaccaaagt gcgcgtcttt ccagttcaaa tcgggccagt tcaaatagag ccggtgcaaa
     2641 acgacacaac ggttgtgccc ggcacccgca tt
//
LOCUS       DASUEG010000018         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_330569, whole genome shotgun sequence.
ACCESSION   DASUEG010000018 DASUEG010000000
VERSION     DASUEG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330569"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..805)
                     /locus_tag="VFX11_00300"
     CDS             complement(227..805)
                     /locus_tag="VFX11_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEX4937084.1"
                     /translation="MLRFLSACGWLLLMGPVAAQPIIEEKTVYYEFSANTKKEIWQQI
                     LAKSPMGATNIAGHHAVNVATTEWTLGARYRLEGGLQRCTLTDVQPHLQITIRMPNWT
                     NKWEADAVLAENWDNYVRMVSSHEDVHRQYAIRMATEYEAALMELGSFRYCRELKEEM
                     EKVRQTILGKFNAHNKWFDAKEFVYQKELVWF"
     gene            1054..>2970
                     /locus_tag="VFX11_00305"
     CDS             1054..>2970
                     /locus_tag="VFX11_00305"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937085.1"
                     /translation="MSFMTYRRLAAVLVLGLSLAGCKITVTTLGEGTVSVVSNNATAC
                     TGDATCYDGGYNRRVELQAVAAPGYTFTGWAGACEGTGPCDVRTLGNRHVIATFKTSN
                     VLEPLMNYVPDFFFSGPWPTDTRRNADGQLDLTDFPLNGGLFARAVKRAAKRSDGFAR
                     NGGILVPLATTWDMDLADMEWSSDSLPGDALQLIRLEPGSPDHGQSIPVTFRLHRSPE
                     LAEDSLLHVQPALGHSLEPGATYALLLFTLDPLGYLPFPVQQGAAMKRIIAHGADGKL
                     GEHWGLVKSFVTENSAFAPEQVAAFTVFTTQGVSDKLPSVRTFVNRQDYATAARNIHF
                     ADADSIQSCNSYGDRNGYLLTLEMDLPNILTGRPPYLTSGGHLNRDAAGTLAANGSIR
                     TSVGIALPCIPPAPGERLPVEIHALPTANDIQQYWNSDRFLYDPMVTTNHRVLRVFIP
                     APYTTDRQYSAGVQSLEWLSNLLNIDIDTAVVAAVLADFNPLNLGAVEPQYLQLGMEV
                     LYLRHLLPHVLDQDFTTDPQQLGYWVGNTDWDFTNLTFAGTSLGALAALQANAIDPVP
                     AKNLILTNMPRPNSIHVNNLVDFLSSQIGSETIHAIERLLGVRFPLSPASPLANLLQT
                     GIDTIDPLNRLDALE"
BASE COUNT          618 a          973 c          811 g          568 t
ORIGIN      
        1 tgatcgaaac ccttcaggta caggctgacg gcatcatcaa acgtatcagc ccacactgac
       61 ggcatgaacg cacaggcaac aagggatgaa acgaacgcaa acacattgat tctgcgcata
      121 tttctatatt ggctcacagc aacaaaggga aggcagcaag gcgctttttt aaagtctagc
      181 agggctttta gtgaccagcc cgaacgccct ggcctgcgct tacccgctag aaccagacca
      241 gctctttctg gtagacaaat tccttggcgt cgaaccactt gttgtgggca ttgaatttgc
      301 ccaggatggt ctgccggact ttctccattt cttctttcag ctcacggcaa tagcgaaagg
      361 aacccagctc catcaaggcg gcttcgtatt cagtcgccat cctgatcgca tactggcggt
      421 gaacatcctc atggctggac accatgcgca cataattgtc ccagttttct gccagtaccg
      481 cgtccgcctc ccatttgttg gtccagttgg gcatgcggat cgtaatctgc aggtggggct
      541 gcacgtcggt cagggtgcat cgctgcaggc ccccttccag acgatagcgc gcacccagcg
      601 tccattcagt ggtggccaca ttcaccgcat gatgcccggc aatgttggtt gcccccatgg
      661 gcgacttggc cagaatctgc tgccagattt cctttttggt gttcgcactg aattcgtaat
      721 agacggtctt ttcttcaatg atcggctgcg cagcgactgg ccccatcaac agtagccagc
      781 cgcacgccga cagaaaacgc aacacaggta acatccttgg tttcaatcga ggtcaatcag
      841 acaaagcagt gcattgcacg gaaactgaat gatacacaaa tcgccggtgt catgccgtgc
      901 cggcgcaggc actgtcccgg cacggccaat ctgctgactt taccgcgtca ccgcgccggc
      961 aggcattgcg cgcggtgaaa gcgctcttct aatatcgtcc gcttagcagg ctgttagtca
     1021 cccataccaa aaaacaaagc agggaagcgc gcgatgtcat tcatgactta tcgccggctt
     1081 gcggcagtcc tcgtactggg gctgtcgctt gccggatgca agatcacggt caccacactt
     1141 ggggaaggca cggtcagcgt cgtcagcaac aacgcaacgg cctgcaccgg cgatgccacc
     1201 tgttacgacg gcggctacaa ccggcgggtg gaactgcagg cagtggcggc accgggctac
     1261 acctttaccg gctgggccgg cgcttgcgag ggcaccggcc cctgcgacgt gcgcacgctg
     1321 ggcaaccgcc acgtgatcgc caccttcaag accagcaacg tcctggagcc cctgatgaac
     1381 tacgtgccgg acttcttctt ttccggcccc tggcccacgg ataccaggcg caatgccgat
     1441 ggccaactgg acctgaccga cttcccgctc aacggcggcc tgttcgcccg cgccgtgaaa
     1501 agagccgcca aacgcagcga cggttttgcc cgcaacggcg gcatcctggt accgctggcc
     1561 actacctggg acatggacct cgccgatatg gaatggtcat cggattccct gcctggggac
     1621 gccctgcaac tgatcaggct ggaacccggt tcgccggacc atggccagag cattcccgtc
     1681 accttccgcc tgcaccgctc ccctgagctg gcggaggaca gcctgctgca tgtgcagcca
     1741 gcgctgggcc acagccttga gcccggcgcc acgtacgcct tgctgctgtt taccctggac
     1801 cccctcggat acctgccatt tccagtacaa cagggcgcgg ccatgaagcg gatcatcgcc
     1861 cacggggccg atggaaagct gggcgagcat tggggcctgg tgaaatcctt cgtcaccgaa
     1921 aattccgcct ttgcaccgga gcaggtggcg gccttcaccg tcttcaccac ccagggcgtc
     1981 agcgacaaac tgcccagcgt gcgcactttc gtgaaccggc aggactatgc caccgcggcc
     2041 cgcaacatcc actttgcgga cgccgacagc atccagagct gcaactctta tggtgatcgc
     2101 aatggctacc tgctgacact ggagatggac cttcccaaca tcctcaccgg cagaccaccc
     2161 tacctcacca gcggcggcca tctgaatcgg gatgctgccg gcacactggc ggccaacggc
     2221 agcatccgca ccagcgtcgg aattgcgctg ccctgcattc cacctgcgcc gggggaacgc
     2281 ctgccggtgg aaatccacgc cctgcctacc gcaaacgaca tccagcaata ctggaattca
     2341 gatcgcttcc tttacgatcc catggtaacg acgaaccacc gcgtgttacg ggtgttcatc
     2401 ccggctccct acaccaccga ccgccaatac agcgccggcg tgcaaagcct ggagtggctc
     2461 agcaacctgt tgaatatcga catcgatact gccgtggtgg cggccgtgct ggcggatttc
     2521 aatccgctga acctgggcgc ggtggaaccg caatacctgc agctgggcat ggaagtgctg
     2581 tacctgcgac acctgctgcc acacgtgctg gaccaggatt tcacgactga tccgcaacag
     2641 ctgggctact gggtgggcaa cacggactgg gacttcacca acctcacctt tgccggcacc
     2701 agtctgggcg cgctggcagc gctgcaggcc aacgcgattg atccagtgcc ggcaaagaac
     2761 ctgatcctga ccaacatgcc gcgccccaac agtatccacg tgaacaatct ggtggatttc
     2821 ctgtcgtcgc agatcggatc ggagaccatc cacgccatcg agcgcctgct gggcgtacgt
     2881 ttcccgctgt cgcccgccag cccgctggca aacctgctgc aaacgggcat cgataccatc
     2941 gaccctctca accggctgga tgcgctggaa
//
LOCUS       DASUEG010000019         4912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2164133, whole genome shotgun sequence.
ACCESSION   DASUEG010000019 DASUEG010000000
VERSION     DASUEG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164133"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFX11_00310"
     CDS             complement(<1..223)
                     /locus_tag="VFX11_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEX4937086.1"
                     /translation="MSSNDTTGKTLLVATALCVFCSIFVAYAAVALKPEQEANKQLDK
                     NKSILAAAGLLDSGKKVKDVFDQRIEVRVV"
     gene            complement(213..1430)
                     /locus_tag="VFX11_00315"
     CDS             complement(213..1430)
                     /locus_tag="VFX11_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEX4937087.1"
                     /translation="MIRKIFDSLQPHFEKGGKYEKYNAVFEMFDTFFYSPSSVTHSAS
                     HVRDGIDLKRIMITVWLCTFPAMFYGMYNVGAQALGYMAENGITAQGDWRFLITDLLI
                     GYNPDSLWDCFIYGAAWFVPIYVVTFAVGIAWEILFAVKRGHEVNEGFFVTSVLFALT
                     CPPTIPLWQVALGISFGVVLGKEVFGGTGKNFLNPALTGRAFLFFAYPASMSGDAVWT
                     AVDGFSGATALSVAAEGGMQALQGQLTWMDAFIGNLQGSVGETSALALAIGGLVLMIT
                     GIASWRIVAGVFAGTAAMGILFNSIGSTTNPMFEIPFWWHYVLGGWALGTFFMATDPV
                     SAAMTDTGKWVFGALIGVMVMLIRVVNPAFPEGMMLAILFANLLAPTIDYFVIQANVK
                     RRLARMASNSSVQ"
     gene            complement(1435..2769)
                     /locus_tag="VFX11_00320"
     CDS             complement(1435..2769)
                     /locus_tag="VFX11_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEX4937088.1"
                     /translation="MRKIKRGLNLPISGAPKQIIEDGPAIRTAAVLGNDYVGVKPTMQ
                     VKVGDRVRKGQTLFTDKKNEGVRFTAPVAGVVAAIHRGHKRVMQSVVIDVEEGGEESF
                     AAYSPTELSQLGHSRVEQNLLASGLWAAFRTRPFSKIPTPGTDPHSIFVTAIDTNPLA
                     ADPAVVIKDEVVAFQHGLDVLSNLSKTDYLCKAAGARIGGNGIAREEEFDGPHPAGLP
                     GTHIHFLDPVHLKKVVWHINYQDVIAIGKLFTTGKLCNQRVIALAGPQVTRPRLIRTV
                     LGASTDELVAGQTEPGENRVISGSVLSGDTAYGPFAFLGRYHLQVTVIREGRERNLLE
                     YVWPGAEKHSVLNTFLSKLTPGKLFPFTSTTNGSQRAMVPVGSYEKIMPLDILPTQLL
                     RALIVGDTETAQQLGCLELDEEDLALCTYVCPGKYEYGPILRDNLTRIEMEG"
     gene            complement(2998..4443)
                     /locus_tag="VFX11_00325"
     CDS             complement(2998..4443)
                     /locus_tag="VFX11_00325"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX4937089.1"
                     /translation="MTDQSKQHLQSWVNREALAESMIPMIGKLYREHSVVTSVYGRPI
                     INRSVIDIIKAHRYVRQLQGEPLDVSESFPVLQALTKLNLGPARIDIGKLATKYRSQG
                     GNLSVEEFVRREVADALDPTITPAFKGRDVVLYGFGRIGRLVARILIEKAGGGDALRL
                     RAIVVRNGGAENDLAKRASLLRRDSVHGSFRGTITIDEENQALIANGNYIQVIYSDAP
                     DSVDYTKYDIHNAIVVDNTGKWRDEAGLGKHLKCPGASQVVLTAPGKGELKNIVMGVN
                     DNEITNDDRILSAASCTTNAIVPVLKVVNDLYGIQYGHVETVHSYTNDQNLIDNYHKG
                     NRRGRSAALNMVITETGAAKAVAKALPELKGKLTGNAIRVPTPNVSMAILNLGLNKEV
                     SKEELNDVLRAASLDGPLRKQIDYVNSPEVVSTDFVGSRYASIVDAESTIVNGNRCVL
                     YIWYDNEFGYSCQVVRMLQKMAGVNYPTFPK"
     gene            4703..>4912
                     /locus_tag="VFX11_00330"
     CDS             4703..>4912
                     /locus_tag="VFX11_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937090.1"
                     /translation="MKTRDRILVTSLELFNLCGEPNVTTIDIANEMDISPGNLYYHFR
                     NKDEIIAELFQQFETSMHDVLQSPQE"
BASE COUNT         1048 a         1502 c         1354 g         1008 t
ORIGIN      
        1 tcaccacccg cacttcaata cgctggtcga acacgtcctt cactttcttg ccggaatcca
       61 gcaaaccggc cgcagcgaga atgctcttgt tcttgtcgag ctgcttgttg gcttcctgct
      121 ccggcttcag cgccacggcg gcataggcca cgaaaatcga acagaacacg caaagcgcgg
      181 tggccaccag cagggtttta ccggtcgtat cgttactgga cactgctatt actggccatg
      241 cgcgccagcc tccgtttcac gttcgcctga atcacgaaat agtcgatggt gggtgccagc
      301 agattggcga acaggatcgc cagcatcatg ccctcgggga aggcgggatt caccacccgg
      361 atcagcatca ccatcacgcc gatcagcgcg ccgaacaccc atttgccggt atccgtcatc
      421 gctgccgaca ccggatcggt cgccatgaag aaggtaccca gtgcccagcc gcccagcacg
      481 tagtgccacc agaacggaat ttcgaacatg ggattggtgg tgctgccgat actgttgaac
      541 aggataccca tcgccgccgt gcccgcgaaa acgccggcaa caatgcgcca ggatgcaatg
      601 ccggtgatca tcaggaccag gccaccgatg gccagtgcca gcgccgaggt ctcacccacc
      661 gaaccctgca agttgccgat aaacgcatcc atccaggtaa gctgtccctg caacgcctgc
      721 atgccgccct ctgctgcgac actgagcgcg gttgcaccgg agaaaccgtc caccgctgtc
      781 cacactgcgt caccggacat actggccgga taggcgaaaa acagaaacgc gcgccccgtc
      841 agcgccggat tgaggaaatt cttgccggtg ccgccaaaca cttccttgcc cagcaccaca
      901 ccgaacgaaa tacccagcgc cacctgccac agcggaatgg tgggcgggca ggtcagcgcg
      961 aacagcaccg acgtaacgaa gaacccttca ttcacttcat gaccgcgctt taccgcaaac
     1021 aggatttccc acgcgatgcc caccgcgaag gtgacgacat aaatgggcac gaaccaggca
     1081 gcaccgtaaa tgaagcaatc ccagaggctg tccgggttat aaccgatcag caggtcagtg
     1141 atcaggaaac gccagtcacc ctgcgccgta ataccattct ccgccatgta acccagcgcc
     1201 tgcgcgccga cgttgtacat gccataaaac atcgccggaa aggtacacag ccacacggtg
     1261 atcatgatgc gcttgaggtc gataccatcg cgtacgtgcg acgcggaatg ggtgaccgac
     1321 gacgggctgt agaagaacgt gtcgaacatt tcgaagacgg cattgtattt ttcgtacttg
     1381 ccgcctttct caaaatgggg ttgcagacta tcgaaaatct tgcggatcac cggctcaacc
     1441 ctccatctcg atgcgcgtca gattgtcccg caggatcggg ccatattcgt acttgccggg
     1501 acagacatag gtacacagcg ccagatcctc ttcgtccagc tccaggcaac ccagttgctg
     1561 tgcggtttcg gtgtcgccca cgatcagcgc ccgcaacaat tgggtgggca gaatatcgag
     1621 cggcatgatt ttttcgtagg aacccaccgg caccatcgcg cgctgactgc cattggtggt
     1681 ggaggtgaac gggaacagct tgcccggtgt cagtttcgac agaaaggtat tcagcaccga
     1741 atgtttttcc gcacctggcc acacgtattc cagcaggttg cgctcgcgac cttcccgaat
     1801 caccgtgacc tgcaagtgat agcgcccgag aaacgcgaag ggtccatagg cggtatcacc
     1861 cgacaacacc gatccggaaa tcacgcggtt ctcacccggt tcggtctggc ctgccaccag
     1921 ttcatccgtg ctggcaccca gcaccgtgcg gatcaaacgg gggcgggtca cctgcggacc
     1981 cgccagcgca atcacgcgct gattacacag cttgccagtg gtgaacagtt tgccgatggc
     2041 gatcacatcc tgatagttga tgtgccacac cacctttttc aggtgcaccg gatcgagaaa
     2101 atgaatatgc gtgcccggca gaccagcggg atgggggccg tcgaattcct cttccctggc
     2161 aatgccgttg ccaccgatgc gcgcgccggc cgccttgcac agatagtccg ttttactcag
     2221 attcgacaac acatccagac catgctggaa cgccaccact tcgtccttga tgacgacagc
     2281 gggatcggcc gcgagcggat tggtgtcgat ggcggtgacg aatatcgaat ggggatcggt
     2341 accaggtgtg ggaatcttgc tgaaaggacg ggtacggaag gcagcccaga gaccggatgc
     2401 cagcaaattc tgctcgacac gggagtgacc gagctggctc agctcggtgg gcgagtaggc
     2461 cgcgaaactc tcttcgccac cctcctccac atcgatcacc accgactgca ttacccgctt
     2521 gtggccccga tgtatggcgg cgacgacacc cgccaccggc gcggtgaagc ggactccctc
     2581 gtttttcttg tcggtaaaga gcgtctgccc tttccttacc cgatcaccca ccttgacctg
     2641 catggtgggc ttgaccccga cgtaatcatt acccagcacg gctgccgttc gaatggcggg
     2701 accatcttcg atgatctgct tcggcgcgcc gctgatgggg agattcagac ctcgcttgat
     2761 ctttctcatg acccctgacc gcttcctgat tcaaacctgg agtgtcccgg attatagcga
     2821 gggttcaatg cgatggatag gttaaatggc cagcaaaagc cggcgcaacc cactattttc
     2881 aatggaaaat tgcggcgtcg tggcgcaaaa aagggcagca actgctgccc ttttccgact
     2941 tgttttcggg gcttgatccg gatcatttat gccaccggac cagcccgttt cactgcatca
     3001 ttttgggaag gtcggatagt ttacgcctgc cattttctgc agcatgcgca ccacctggca
     3061 gctgtaaccg aattcgttgt cgtaccagat gtacagtaca caacggttgc cattcacgat
     3121 ggtggactcg gcatccacga tgctggcata gcgggagcca acaaagtcgg tggacaccac
     3181 ttccggcgag ttcacatagt cgatctgctt gcgcagcgga ccgtccaggg aagcagcgcg
     3241 caatacgtcg ttcagctctt ccttcgacac ttccttgttc aggcccaggt tcaggatcgc
     3301 catggatacg ttgggcgtcg gaacgcggat cgcattgccg gtcagcttgc ctttcagttc
     3361 cggcaacgcc ttggcaacgg ccttggcggc accggtttcg gtgatcacca tgttcagcgc
     3421 cgcgctgcgg ccacgacgat tgcccttgtg gtagttgtcg atcaggttct ggtcgttggt
     3481 ataggagtga acggtctcca cgtggccgta ctggatgccg tacagatcgt tcaccacttt
     3541 caataccggc acgatggcgt tggtggtgca gctggccgcc gacaggatgc ggtcgtcgtt
     3601 ggtgatttcg ttgtcgttca cacccatcac gatgttcttc agctcgccct tgccaggcgc
     3661 agtcagcacc acctggctgg caccggggca cttcaggtgc ttgcccaggc cggcttcgtc
     3721 gcgccacttg ccggtgttgt ccaccacgat ggcgttatga atgtcgtact tggtgtagtc
     3781 aacgctatcc ggcgcatcgg aatagatcac ctggatgtag ttgccgttgg caatcagcgc
     3841 ctggttttct tcatcgatgg taatggtgcc acggaaggaa ccgtgcacag aatcgcggcg
     3901 cagcaggctg gcacgcttgg ccaggtcgtt ctctgcgcca ccattacgta ccacgatggc
     3961 acgcagacgc agggcatcgc caccgccggc cttttcaatc agtatgcgcg ccaccagacg
     4021 gccaatgcgg ccaaatccgt acagcaccac gtcgcggcct ttgaacgccg gagtaatggt
     4081 ggggtccagc gcgtcggcca cttcgcgccg cacgaactct tccacgctca ggttgccgcc
     4141 ctggctgcgg tatttggtgg ccagcttgcc gatatcaatc cgggccgggc ccaggttcag
     4201 cttggtcagc gcctgcagca ccgggaagct ttccgaaaca tccagcggct cgccctgcag
     4261 ctggcggacg taacggtggg ccttgatgat gtcgatcacc gaacggttga tgatcgggcg
     4321 gccatacacg gaggtcacca cgctgtgctc gcggtacagt ttgccgatca tggggatcat
     4381 ggattcggcc agggcctcac ggttaaccca ggattgcaaa tgctgttttg actggtctgt
     4441 cacggaacgt caccttttat ggagtgccaa caatggaatg ccgacctagc accgtaagcc
     4501 ctgacggcgg tcacggttcg tcgctgtggg gcgcatatta tcttgtcatt gaaaccattt
     4561 atcaatccga gcggcgagcc atcgacccag cctgtttaac caactgaaaa aacaaccgat
     4621 taatgacttg atcgggacgc tgacgtattc ttaacgccag atcatcgatg acctcatctc
     4681 cttagggata gccgaccccg gcatgaaaac acgcgatcgc atcctggtta ccagcctgga
     4741 actgttcaac ctgtgtggtg aacccaacgt caccaccatc gacattgcca atgaaatgga
     4801 catcagtccg ggcaatctct actaccactt ccgtaacaag gacgaaatca tcgccgagct
     4861 gttccagcag ttcgaaacca gcatgcacga tgtgttgcag tcgccccagg ag
//
LOCUS       DASUEG010000020         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_838416, whole genome shotgun sequence.
ACCESSION   DASUEG010000020 DASUEG010000000
VERSION     DASUEG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838416"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VFX11_00335"
     CDS             complement(<1..1719)
                     /locus_tag="VFX11_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015827456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4937091.1"
                     /translation="MSHSPIVGGGPKKLLYTLSTLRQLGLRNAAKALTSHNACKACGL
                     GMGGQRGGMTNELGEFPAVCNKSVQAQSTDIQPAIPADLFAQHPIADFSQLSDRELEH
                     LGRLDTPLYKSASDNRYHPITWDSAFHLMAERFAATTPDRSFFYASGRSSNEAGFVLQ
                     LLARLYGTNNVNNCSYYCHQATGIGLYNAIGTGTATVTLEDVTHADLIFVIGANPSSN
                     HPRLVHQLKNCRERGGDVIVINPAKEPGLVRFAVPKSVTSMLSGGSWIASDYVQPIIN
                     GDLALLKGMAKALCEQNRIDVDFIAQHTEGFDAFARDLTLTSWQSLEAGSGISADRMR
                     ALAQRYAQAERAIFAWGMGITHHLNGVENVEAIANLALLRGMIGKPAAGLLPLRGHSN
                     VQGIGTVGVKPLLPDDVLAHLEQHFNLKLPTREGMDTLACLEAAHAGNIDACLLLGGN
                     LYAATPNSQWARAALDRIGFKVYLTTTLNRGHVTGMENSAALLLPVTARDEEWQPTTQ
                     ESMFNYVRLSDGGIRRLEQARPESHILSELGARLLPDGPVDFLAFQSHENVREAIAAS
                     VPGMEQL"
     gene            1784..2626
                     /locus_tag="VFX11_00340"
     CDS             1784..2626
                     /locus_tag="VFX11_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003197627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEX4937092.1"
                     /translation="MNGVLVLLYWQIGRDILARQAQQGWGAKVIERLAQDLRAAFPDM
                     KGFSPRNLKYMRAFAEAWPDSEFVQQAAAQLPWGHNLVLLDRLPGPETRRWYAAKAIE
                     HNWSRNVLVMQIESRLLERSSKTITNFEASLPKPQSDLARESIKDPYRFDFLGLTDDA
                     QEREIENALTKHVTEFLLELGAGFAFVGRQVLLDVGGDEFFIDLLFYHLKLRCYVVVE
                     LKGGKFKPEHLGQLSFYLTAVDTQIKHSQDGPTIGLLLCKSKNKVVAEYALRNVNQPL
                     GVAD"
BASE COUNT          514 a          743 c          844 g          555 t
ORIGIN      
        1 gagctgttcc atgccgggca cgctggcggc aatggcttcg cgcacatttt cgtgggattg
       61 aaacgccagg aaatccaccg ggccatctgg cagcagacgc gcgcccagtt cgctgaggat
      121 atgactttcc ggccgcgcct gttccagccg gcgaataccg ccgtcgctca ggcgcacgta
      181 attgaacatg gattcctggg tggtgggctg ccattcttcg tcgcgcgcgg tgacgggcag
      241 cagtaacgcg gcgctgtttt ccatgccggt gacatggccg cgattgagcg tggtggtcag
      301 gtacaccttg aagccgatgc gatccagcgc tgcgcgtgcc cactggctgt tcggcgtggc
      361 cgcgtacaga ttgccgccca gcagcaggca ggcgtcgata ttgccggcgt gggccgcttc
      421 caggcaggca agcgtgtcca tgccttcccg ggtgggcagt ttgagattga agtgttgttc
      481 caggtgggca agtacgtcgt ccggcaacag tggttttacg cccaccgtgc cgatgccctg
      541 cacattgctg tggccgcgca ggggcaggag tccggcggca ggtttgccga tcatcccgcg
      601 cagcaatgca aggttggcga tggcctcgac gttttccacg ccattcaggt gatgggtgat
      661 gcccataccc caggcgaaaa tcgcgcgctc ggcctgggcg tagcgttgcg ccagggcgcg
      721 catgcggtct gcactgatac cgctgccggc ttccagcgat tgccaggatg ttagcgtcag
      781 atccctcgca aatgcatcga acccttccgt atgctgcgcg atgaaatcca catcgatccg
      841 gttctgttcg cacagcgctt tcgccatgcc cttcagcaac gccagatcgc cattgatgat
      901 cggctgcaca tagtcgctgg caatccacga gccaccactg agcatggacg tgacgctttt
      961 cggcacggca aaccgcacca ggccgggttc ctttgcggga ttgatcacga ttacatcgcc
     1021 gccccgctcc cggcagtttt tcagctgatg caccaggcgc ggatggttgg acgagggatt
     1081 cgcgccgatc acaaagatca gatccgcgtg ggtgacatct tccagcgtca ccgtggcggt
     1141 gccggtgccg atggcgttgt ataaaccaat gcccgtggcc tgatggcagt aatacgagca
     1201 gttgttgacg ttgttggtgc cgtacagacg cgccagcagt tgcagcacga agcccgcctc
     1261 gttggatgaa cggcccgagg cgtagaaaaa actgcggtcg ggtgttgtgg cggcaaagcg
     1321 ttcggccatg aggtgaaacg cgctgtccca ggtgatcggg tgatagcggt tgtcgctggc
     1381 ggatttgtag agtggcgtgt cgagccggcc caggtgttcc agttcgcggt cactcaactg
     1441 cgagaaatca gcaatcggat gttgcgcaaa aagatcggcg ggaatggcgg gttggatgtc
     1501 ggtggactgg gcctgtacgc tcttgttgca gacggcggga aattccccca gctcattggt
     1561 cataccaccg cgctggccgc ccatacccag cccgcacgct ttgcaggcgt tatggctggt
     1621 cagggctttg gccgcattgc gcagccccag ctgccgcagc gtgctgaggg tatagagcag
     1681 ctttttcggt ccaccgccaa caatagggga gtgggacatg ggccattcct ggagagcgtg
     1741 ataatcgcga cagtagcgga gatggcgaga aaagggaaat acgttgaatg gcgtattggt
     1801 gctgctgtac tggcagattg gacgcgacat tctggcccgg caggctcagc agggctgggg
     1861 cgcgaaagtg atcgaacggc tggcacagga tctgcgggcg gcatttcctg atatgaaggg
     1921 gttttctccc cgcaatctca agtacatgcg tgcgtttgcc gaggcttggc cggattcgga
     1981 atttgtgcaa caggctgctg cacaattacc ttggggccac aatctggttc tgttggatag
     2041 gctccccggc cccgaaaccc gccgctggta tgctgccaaa gccatcgaac ataactggtc
     2101 acgcaatgtt ctggttatgc agattgaaag ccggttgctg gaaaggagca gcaagacaat
     2161 aaccaatttc gaggccagcc tgcccaagcc gcaatccgat ctggcccgcg aatccatcaa
     2221 agacccctat cgcttcgatt ttctgggcct gaccgatgat gcccaggagc gcgagatcga
     2281 aaacgccctg acaaagcatg tcaccgaatt cctgctggaa ctcggtgctg gctttgcgtt
     2341 cgtcgggcgg caagtcctgc tggacgtcgg tggcgacgag ttcttcatcg acctgctgtt
     2401 ctatcactta aaactgcgtt gctatgtggt ggtggaactg aagggtggca agttcaaacc
     2461 ggagcatctg ggtcaattga gcttttacct cacggccgtg gatacacaga tcaaacactc
     2521 gcaggatggt cctaccatcg gcctgctgct gtgcaaaagc aagaacaagg tggtggccga
     2581 gtacgcgctg cgcaatgtga accagccgct gggcgtggcg gactagcagt tggtggaatc
     2641 cttgccgccg gagctg
//
LOCUS       DASUEG010000021         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_330486, whole genome shotgun sequence.
ACCESSION   DASUEG010000021 DASUEG010000000
VERSION     DASUEG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330486"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="fadH"
                     /locus_tag="VFX11_00345"
     CDS             complement(<1..725)
                     /gene="fadH"
                     /locus_tag="VFX11_00345"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003904566.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEX4937093.1"
                     /translation="MNAFYPHMLQPLDLGFTTLRNRVLMGSMHTGLEDRFYHIDKLAS
                     YFAERARGGVGIIVTGGYSPNRRGELLPLGSRMDNKVTAMLHRHVTGKVHNEGGKIVL
                     QLLHAGRYGYTPYSVSPSGIKSPITPFKPWKMSGKQVENTIADFARAAELAKLAGYDG
                     VEIMGSEGYLLNQFLCARTNKRTDKWGGSAENRTRFPLEVVRKVRAAVGREFIIIYRM
                     SLMDLVEDGQSWDEVEMLAKGVEA"
     gene            864..1694
                     /locus_tag="VFX11_00350"
     CDS             864..1694
                     /locus_tag="VFX11_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4937094.1"
                     /translation="MSASPLPEKLRTDKLIGVPETMLIPLFARAVETPRPDAICRDPQ
                     AVELVNRIDYDFSKFDPATATVLGIAIRTRIFDDMARAYIEQHPDCVVINIAAGLDAR
                     FYRVDNGTLRWYELDLPEAIELRRQFFSESERHRFLAANALDPAWLEQIEHSKHTLVI
                     IEGLLMYFDESDVKFLLTLLAERLPGCETLVEVLGHSQALRTENNDMVNKTNAAFKWG
                     IRNAADIGDWHPVLQYVNDISIYDRHDDRWHQLPLQWPAPLAKLRNTANRIVHLRSRG
                     "
     gene            complement(1816..3096)
                     /locus_tag="VFX11_00355"
     CDS             complement(1816..3096)
                     /locus_tag="VFX11_00355"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4937095.1"
                     /translation="MKVDQVRKVAIIGGNRIPFARSNTAYSHASNQDMLTAALNGLVE
                     RYKLQGKLMGEVVGGAVMKHSRDFNLVRESVLSTQLAPETPCYDIQQACGTGLEAAIL
                     VANKIALGQIDCGIACGVDTTSDAPIGVSEGLRKILLDLNRAKTNQERMKLIAKIRPR
                     HLMPLIPANSEPRTGLAMGDHCEITAKEWNIPRAEQDKLAFESHTKMAAAYERGFFKD
                     MITPFMGLERDNNMRGDTKMEKLASLKPCFDKENGTMTAANSTPLTDGASSVLLASEE
                     WAKKNNLPILAYMTYCETAAVDFVGKKEGLLIAPAYAVPRLLDRAGITLQDFDFYEIH
                     EAFAAQVLYTLKAWETESFCKQKLGRDKVLGSIDRAKLNVNGSSLAAGHPFAATGGRI
                     IHTLAKLLNEKGSGRGLISICAAGGQGVTAILEK"
     gene            3416..>3709
                     /locus_tag="VFX11_00360"
     CDS             3416..>3709
                     /locus_tag="VFX11_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEX4937096.1"
                     /translation="MADLYESLLSNNVGRSLLNSLNLPVPTPLERHSSTQTSFLSGNV
                     LVGAAPGGRMLDVIAATLKQSNATALVAQNAANIADIKQAGDKAELKFTNVDPA"
BASE COUNT          761 a         1163 c         1006 g          779 t
ORIGIN      
        1 gcttccacgc ctttggccag catttccact tcatcccagc tctggccgtc ttccaccaga
       61 tccatcagcg acatgcgata aatgatgatg aactcacggc cgaccgctgc gcgcaccttt
      121 cgcaccactt caagcggaaa ccgcgttctg ttttccgcac tgccacccca tttgtctgtg
      181 cgtttgttgg tgcgcgcaca gaggaactgg ttgagcagat aaccttccga tcccattatt
      241 tcgacgccat cgtaaccggc cagtttggcc agctcggcgg cgcgggcaaa atcggcgatg
      301 gtgttttcaa cttgcttgcc gctcattttc cacggcttga acggcgtgat gggcgacttg
      361 atgcccgacg gcgacacact gtacggcgtg taaccgtaac gccctgcgtg taacagctgc
      421 agtacgatct tgccgccttc gttgtgcacc ttgccggtga catggcggtg cagcatcgcg
      481 gtgaccttgt tgtccatgcg cgaacccagc ggcagcagtt caccgcgccg gttgggggaa
      541 taaccacctg tcacgatgat gcccaccccg ccccgcgcgc gctcggcaaa atagctggcc
      601 agcttgtcga tatggtagaa gcggtcctcc agccccgtgt gcatggaccc catcagcacg
      661 cgattgcgca gcgtggtaaa gcccagatca aggggttgca gcatgtgcgg gtaaaaggcg
      721 ttcatccatc ttcctcatca atcaacacag tgaatggcaa taaaaaaacc tgccacaatc
      781 ctagcccagt tttgttgcgg aggggctcgc gaattgctaa cgtgaccaaa cagtcaagtc
      841 actagaatgc caaggaaacc gacatgtccg ccagccccct tccggaaaaa ctccgcacag
      901 acaaactgat cggcgtacct gaaaccatgc tcatccccct gttcgcacgc gcggtggaga
      961 cgccgcgtcc cgacgccatc tgccgcgacc cgcaggcggt cgaactggtg aaccgcatcg
     1021 actacgactt cagcaaattc gatcccgcca ccgccaccgt cctgggcatc gccatccgca
     1081 cccggatttt cgacgacatg gcacgcgcct atatcgaaca gcatccggat tgcgtcgtca
     1141 tcaacattgc tgccgggctg gacgctcgct tctaccgcgt cgacaacggc acgctgcgct
     1201 ggtatgaact ggatctgccg gaagccatcg aactgcgccg gcagtttttc agcgaaagtg
     1261 aacgtcaccg tttcctggcc gccaacgccc tggacccggc ctggctggag cagatcgaac
     1321 actccaaaca tacgctggtc atcatcgaag gtctgctgat gtatttcgat gaaagcgatg
     1381 tgaaattcct gctgacgctg ctggccgaac gtctgccggg ctgcgagaca ctggttgaag
     1441 tgctcggcca ctcgcaggcg ctgcgcaccg aaaacaacga catggtgaac aagaccaatg
     1501 ccgccttcaa atggggcatc cgcaatgcgg cagacatcgg cgattggcat ccggtactgc
     1561 agtatgtgaa cgacatttcc atttacgacc ggcacgacga ccgctggcat caactgccgc
     1621 tgcaatggcc cgcgccactg gcgaagctgc gcaatacagc gaaccgcatc gtgcatctgc
     1681 gttcccgcgg ctgatcggtt cccctgtcag ccacatcacg gtcagcctca tcacaaccac
     1741 tgtttagcca cctgcaaaaa aggcgactct tttcagtcgc ctttttcgtg ttgcgcctga
     1801 ttccagaccc ggtgcttatt tttccagaat cgcggtcacg ccctgaccac cggccgcgca
     1861 gatggagatc aggccacggc ccgagccctt ctcgttcagc agtttcgcca gcgtgtgaat
     1921 aatgcggccg ccggtagctg caaacgggtg acccgccgcc agcgaggaac cgttcacgtt
     1981 cagtttggcg cgatcgatac tgcccagcac tttgtcgcgg cccagcttct gcttgcagaa
     2041 ggattcggtt tcccaggctt tcagcgtgta cagcacctgc gccgcaaacg cttcatggat
     2101 ttcatagaaa tcgaaatcct gcagagtgat gccagcgcga tccagcagac gcggcacagc
     2161 ataggccggc gcgatcagca gtccttcttt cttgcccacg aaatccaccg cagcggtttc
     2221 gcagtacgtc atgtacgcca gaatcggcag gttgtttttc ttcgcccact cttcactggc
     2281 cagcagcaca gacgatgcgc catcggtcag cggcgtggaa ttcgcggcgg tcatggtgcc
     2341 gttttcttta tcgaagcagg gcttcaggct ggcgagtttt tccatcttgg tgtcgccgcg
     2401 catgttgtta tcgcgctcca ggcccatgaa cggcgtgatc atgtccttga agaagccgcg
     2461 ctcgtaggct gccgccattt tggtgtggct ttcgaatgcc agtttgtcct gttcggcgcg
     2521 gggaatgttc cattccttgg cagtgatttc gcaatggtcg cccatggcca ggccggtgcg
     2581 cggttcgctg ttggcaggaa tcagcggcat caaatgacgg ggacggattt tcgcaatcag
     2641 cttcatccgc tcctggttgg tcttggcgcg gttcaggtcc agcaggatct tgcgcaggcc
     2701 ttcgctgaca ccgatgggcg cgtcagaggt cgtgtccacg ccgcaggcaa taccgcagtc
     2761 gatctggccc agggcgatct tgttcgccac cagaatcgct gcttccagac cggtgccaca
     2821 ggcctgctgg atgtcatagc agggcgtttc cggcgccagc tgcgtgctca gtacggattc
     2881 acgcaccagg ttgaaatcgc gggagtgctt catcaccgcg ccgcccacca cttcacccat
     2941 cagcttgcct tgcagcttgt aacgctcgac cagtccattc agcgccgccg tcagcatgtc
     3001 ctggttgctg gcatgggagt aagcggtgtt ggaacgtgca aagggaattc ggtttccgcc
     3061 aatgatggcc accttgcgaa cttgatctac tttcatggaa aggcttcctg tcgagtggat
     3121 gccctaacaa tgagggcata aagggttgat ctgcctgaac ggatataaga gtcggcaccg
     3181 gctggattcc ggattacggg gcctagtcta gccgggtgtc atcccgaaac attggcagaa
     3241 ctgacccacg gccatttaaa cggggtcaat tcaatttgga atccaagtca tttactatac
     3301 gcgccgacta ccctgcgctt gcagccctgc cacagcccac ggattacgcg gcaccggcgg
     3361 ctgcaaagcg gccacaacta taccacacca acattaccca cacaggatca gaaccatggc
     3421 cgatctgtac gaatccctgc tcagcaacaa cgttggacgc tcgcttctca attcgctgaa
     3481 tctgccggtt cccaccccgc tggagcgcca ttcttcaacc caaacctcgt tcctcagcgg
     3541 caacgtgctg gtgggcgcag cccctggcgg ccgcatgctg gacgtgatcg cggcgacgct
     3601 gaaacagagc aatgccaccg cactggtcgc gcagaatgca gccaatatcg ctgacatcaa
     3661 acaggctggc gacaaggcgg agctgaaatt caccaacgtc gatcctgcc
//
LOCUS       DASUEG010000022         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1678131, whole genome shotgun sequence.
ACCESSION   DASUEG010000022 DASUEG010000000
VERSION     DASUEG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678131"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFX11_00365"
     CDS             complement(<1..523)
                     /locus_tag="VFX11_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate dioxygenase"
                     /protein_id="HEX4937097.1"
                     /translation="MPVNSPTPQSDSTPNDKLNLSRRKALGALGIVSSALLFGCGADS
                     TSTEETTDDSDTGTGSDDTTTSTGDGTCTLIPQETQGPYPLLAILSNSAMVRSDITEG
                     RSGVPLTVNLKLVNVNDSCAPVTSASVYIWHCDKDGLYSGYSQPGGNTVGETFCRGIQ
                     DVDSNGEVTFTTIY"
     gene            complement(659..1225)
                     /locus_tag="VFX11_00370"
     CDS             complement(659..1225)
                     /locus_tag="VFX11_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937098.1"
                     /translation="MNWNDRNYFSIDYFDDLVAAIRSHAFPLLGDVPPRSEQIGHWVV
                     ERVDHVLAPNPAATLSPELMAKAIHAFEVCSEFFADEVTAYFREHAGNHAFHGLFHTS
                     NLDLYAMWCRIAKRTTSSWHSEENWLHKLACALIRDVDFGDKMTEAHTCCSQGMSRKQ
                     TLDQIEQHHRELGSYNTSAKSLLLLDLL"
     gene            1564..>3284
                     /locus_tag="VFX11_00375"
     CDS             1564..>3284
                     /locus_tag="VFX11_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEX4937099.1"
                     /translation="MNSHDPTSPFSWKALDDVPCNAAGNPPLPAAPDPLRRDLLKGGI
                     GVALLGFFGSSASACAAARSNAPLQGLLGFTGIEPQTDPAFDRIVIAPGYQARAFFSW
                     GDPVLPGAPVWQPDAADDWRAQLQQAGDNHDGMHYFPFPDAPNDHGLLVINHEFVSAT
                     LHPQGMTRVDGKRPLGEVKKEQAAHGVSVIEVRKDVAGHWQRVSDSRFNRRLSALTPM
                     AIGGPLAGHDLMKTASDPEGRTVLGTIANCAMGFTPWGTYLICEENWHLYFVNRDAAD
                     HARRVSHWRYGISSADGSRVYGWETADLRFDATPQSGQPFGGHVQEPHRFGWVVEFDP
                     FDADCVPVKRTALGRFCREGASVAQGPDGRLAIYSGDDTKGEYIYKFVPSGRYNPDDP
                     AANRHLLDSGTLYVAQFRAGGEGAWKALVFGQNGLVPANGFSSQEEILLNARAAADQA
                     GATPMDRPEWVAVHPQTREVYVTCTNNDERGSTDPVDAANPRHRNLHGQILRWREAGA
                     DPTATRFQWDIFLLAGEPAGAKDSEGKPVDDNQVGTIRGDLFSSPDGLAFDAAGRLWI
                     ETDYDDRA"
BASE COUNT          592 a          953 c         1055 g          684 t
ORIGIN      
        1 gatagatcgt cgtgaaggtc acttcgccgt tgctgtccac gtcctggatg ccgcggcaga
       61 aggtttcgcc cacggtgttg ccgccgggct ggctgtaacc ggaatagagg ccgtccttgt
      121 cgcagtgcca gatgtaaacc gacgcactgg tgacgggcgc gcagctgtcg ttcacgttca
      181 ccagtttcag attgaccgtc agtggtacgc cggatctgcc ttcggtgatg tcgctgcgca
      241 ccatcgcgga attgctcaga atcgccagca ggggataggg cccctgggtt tcctggggaa
      301 tcagggtgca agtgccgtca ccggtgcttg tggtggtgtc gtccgagccg gtgccggtat
      361 cgctgtcgtc agtggtttct tcagtgctgg tactgtctgc gccgcagcca aacaggagtg
      421 cactgctgac gatgcccagt gcgcccagcg ccttgcgccg gctcaggttc agtttgtcgt
      481 tgggtgtgga atcggattgg ggggtggggg aattgacagg catggcttgc gctctcgtgc
      541 ggaatcgagt caccacgcta tgattcaatt gtgcagcaat tggtgagcaa tcgcgcaagt
      601 gcgaccggcg acgcgcactt gcacgtcaac tgtgatccgt ttctccgcgc aatgcgtgct
      661 atagcagatc cagcagcagc agggattttg cgctggtgtt gtaggagccc agttcgcggt
      721 ggtgctgttc gatctggtcc agtgtctgct tgcggctcat tccctggctg cagcaggtgt
      781 gcgcttccgt catcttgtct ccgaaatcga catcgcggat cagtgcgcag gccagtttgt
      841 gcaaccagtt ttcctcggaa tgccagctgc tggtggtgcg tttggcgatg cggcaccaca
      901 tggcgtacag atccagattg ctggtgtgga acaggccatg gaaagcatgg ttgccggcat
      961 gctcgcgaaa atacgcggtg acttcatcgg caaaaaattc cgaacagact tcaaacgcgt
     1021 ggatggcttt ggccatcagc tccggtgaca gggtagcagc gggattgggt gccagcacgt
     1081 ggtccacgcg ctccacaacc cagtggccga tctgctcgga ccgtggcggt acgtcgccca
     1141 gcaatggaaa agcgtgggat cggatggccg ccaccagatc gtcaaaatag tcgatgctga
     1201 aatagtttcg gtcgttccag ttcatgtgct gtctccgttg tattttcggt tgatgcgccc
     1261 ccgcgtttac caaaataccg gtatctttcc atgacagatt tgtgaccaaa attaatgcaa
     1321 ctgaccgcgg gcgttggcgg tctgggccgg attctggacc ccttgtctgc agcccgtcaa
     1381 ctggccctgg cgccgcttga gggactattc cagcgggcat tgacctgcgt cggaatgcag
     1441 cgcacctggg tgcacgcgcc gccgcgcacc gtgaatgcga atgtcatttc agtcaggtaa
     1501 atggattcga cagatgatgg cggcggcgtt taaatgatcc ggtgcgcggc aggagaacat
     1561 tccatgaatt cccacgaccc tacttcaccg ttcagctgga aggcgctgga tgacgtgcca
     1621 tgcaatgcgg ctggcaatcc acccttgccg gccgcgcccg atccgctgcg acgggatctg
     1681 ctcaagggcg gcatcggtgt tgcgctgctt ggctttttcg gtagctcggc gtcggcctgc
     1741 gctgccgccc gcagtaatgc acccttgcaa ggcttgctcg gtttcaccgg catcgagccg
     1801 caaaccgatc cagcgttcga ccgcattgtc attgcgccgg gctaccaggc gcgtgctttt
     1861 ttttcctggg gcgatccggt gctgcctggc gcgccggtgt ggcaaccgga tgctgcggat
     1921 gactggcggg cgcagttgca gcaggcgggc gacaatcatg atggcatgca ttattttccg
     1981 tttccggatg cgcccaacga tcacggcctg ctggtaatca atcatgaatt cgtcagtgcc
     2041 acgttgcatc cgcagggcat gacccgtgtt gatggcaaac gcccgctggg ggaagtgaaa
     2101 aaagagcagg cggcccacgg cgtcagcgtg atcgaggtgc gcaaggatgt tgccggccac
     2161 tggcagcggg tatcagattc ccgtttcaac cgtcgcctgt ccgcgctgac gcccatggcg
     2221 atcggcggac cgctggccgg acatgatctg atgaaaaccg ccagcgatcc ggaaggtcgc
     2281 acggtgctgg gcaccatcgc caattgcgcc atgggattca cgccctgggg cacctacctg
     2341 atctgcgagg aaaactggca cctgtatttc gtcaatcgcg atgcggccga tcatgcgcgc
     2401 cgggtgtcgc actggcgtta tggcatttct tctgcagatg gcagccgggt ctacggctgg
     2461 gagaccgccg atctccgctt cgatgccacg ccgcaatcgg gccagccttt cggcggccat
     2521 gtgcaggaac ctcaccgctt tggctgggtg gtggaattcg atccgtttga tgccgactgt
     2581 gtgccggtga aacgcacggc gctggggcgt ttctgccgtg aaggcgccag cgtggcgcag
     2641 ggcccggacg gacgtctggc aatctattcc ggcgacgaca ccaagggcga atacatctac
     2701 aaattcgtgc cgtccggccg ctacaacccg gacgacccgg ccgccaaccg ccacctgctg
     2761 gacagcggca ctttgtatgt cgcgcaattc cgggctggcg gggaaggggc gtggaaagcg
     2821 ctggtgttcg gccagaacgg tctggtgcct gccaacggtt tcagcagtca ggaagagatc
     2881 ctgctgaacg cccgcgccgc cgccgaccag gcgggtgcca cgccgatgga ccggccggaa
     2941 tgggtggctg tgcatccgca gacgcgcgag gtctatgtca cctgcaccaa taacgatgaa
     3001 cgcggcagca ctgatccggt cgatgcagcc aatccacgtc accgcaacct gcatggccag
     3061 atcctgcgct ggcgcgaagc cggtgccgat cccacggcca cccgctttca gtgggacatc
     3121 tttctgctgg cgggcgaacc cgccggagcg aaggattcag aaggaaaacc ggtagacgac
     3181 aatcaggtgg gcactatccg gggtgatctt ttttcctcgc cggacggact ggccttcgac
     3241 gcggcaggcc ggctgtggat cgaaacggat tacgacgaca gggc
//
LOCUS       DASUEG010000023         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_993013, whole genome shotgun sequence.
ACCESSION   DASUEG010000023 DASUEG010000000
VERSION     DASUEG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993013"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFX11_00380"
     CDS             complement(<1..961)
                     /locus_tag="VFX11_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4937100.1"
                     /translation="MKILFLSHYFPPEVNAPATRTFEHCVRWVRQGHEVTVVTCNPNH
                     PRGVLYPGYRNRLWSVEDHNGIRVIRLLTLLSANKGFGLRTLNYIAFMLMCVLLCWRF
                     SRPDVVISTSPQFFNGLAGYCVARLRRARWVLEIRDLWPESIVAVGAMKEGLVTALLY
                     RLELFCYRACDLIVPVTDSFRDYMVAKGINGEKIRVIKNGVDLDLFSASATENDSDVD
                     ALLAQHGLNGKFIAAYVGTHGMAHHLDTVLLAAQKIRHRTDIAFLLVGDGAERDRLLQ
                     MAGDMQLPNLVMLDQMPKRKMPALWSRVQVSLILLKKSDTFKSV"
     gene            complement(964..>2241)
                     /locus_tag="VFX11_00385"
     CDS             complement(964..>2241)
                     /locus_tag="VFX11_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX4937101.1"
                     /translation="WIKYFSAREQAAGSLSEEWLFSLVQQGEWLNRNIEYHIRANHLF
                     KNIVAMIWFCAFMQSEPTYRAWLKRYCDLLVRELNEQFNDDGGHYERSPMYHAICLED
                     CMDLYNLLHNAGVDHPCMARLVSVIQRGLSFLKLLSFADDKVALFGDSTLHGCHGWKT
                     LSAYWRKLQGNETEADKTGPQKLVHCADTGFVIRETPYYKIIISTGGVSPGYQPGHTH
                     CDMGSFELCTPQGRLVVDSGVSQYAQGELRRYSRSSLAHNVVMVNGDNQHELWGEFRV
                     GARASIELLKLSAEPTSCGDDADVILRISSHPACGTPWRQTRAYHLGENQIDIVDIVQ
                     SGDNASIESNFHLHPDVDIRAVGDGFNLRLAGESAARVVVSDPANTTVEHSYYCPDFG
                     VVMNGQRICLQKRGSATNRMAVRIELDPAKQWC"
BASE COUNT          526 a          660 c          593 g          462 t
ORIGIN      
        1 tgacggactt gaacgtatcg cttttcttga gaaggatcag ggatacctgt acccgggacc
       61 agagagccgg catcttgcgt ttgggcatct ggtccagcat gaccagattg ggcaactgca
      121 tgtcaccggc catttgcagc agacgatcac gctcggcacc atcgcccacg agaagaaagg
      181 cgatgtcggt gcgatggcgt attttctgcg ccgccagcag cacggtatcg agatggtgcg
      241 ccatgccatg ggttccgaca tacgccgcaa tgaattttcc attcagacca tgctgtgcaa
      301 gcaatgcatc cacgtcactg tcattttccg tggccgacgc ggaaaacaga tcgagatcga
      361 caccgttctt gatgacccgg atcttctcac cgtttatgcc tttcgccacc atgtaatccc
      421 tgaaggaatc cgtcaccggt acaatcagat cacaggctcg ataacagaac agctccagcc
      481 ggtacagcaa agcggttacc aggccttcct tcatcgcacc gacggcgact atggactccg
      541 gccatagatc gcgtatttcc agcacccagc gcgcgcgccg caaccgcgcc acacagtagc
      601 ctgccagccc gttgaaaaac tggggcgagg tggaaatcac aacgtctgga cgactgaagc
      661 gccagcaaag caacacgcac atcagcatga acgcaatgta gttcaatgtc cgcaggccaa
      721 atcccttatt ggctgacagc agggtcagca ggcgaatcac ccggatgccg ttgtgatcct
      781 ctacgctcca caagcggttt cgataacctg gatacaggac accgcgggga tgattgggat
      841 tgcaggtaac aaccgtcact tcatgtccct gccgtaccca gcgcacgcaa tgctcgaagg
      901 tgcgggtggc cggcgcattc acttctggtg gaaagtaatg agagagaaat agaattttca
      961 tgctcagcac cactgcttgg ccgggtccag ttcgattctg accgccattc tgttcgtagc
     1021 ggaaccgcgc ttctgcagac aaatacgctg gccattcatg acgacgccaa aatcgggaca
     1081 gtaataggag tgctcgaccg tggtgttggc aggatccgaa accactaccc tggcagccga
     1141 ttcccctgca agccgcaaat tgaagccgtc acccaccgcc cggatgtcaa catccggatg
     1201 cagatggaaa ttggattcaa tactggcgtt gtcgccggac tggacgatat ccacgatgtc
     1261 gatctgattc tccccaagat ggtaggcccg cgtctggcgc cacggcgtgc cacatgcggg
     1321 atgggagctg atgcgcagaa tgacatcagc gtcatccccg caactcgtcg gctctgccga
     1381 cagtttcaac aattcaatgg aagcgcgagc ccccactctg aattcgcccc agagctcgtg
     1441 ctgattgtca ccgttgacca tgacaacatt atgagccagc gacgaacgcg aatagcgtcg
     1501 caactctccc tgcgcgtact gactgacgcc actgtccact accagccggc cctgcggggt
     1561 gcagagttca aaactgccca tgtcacagtg tgtgtggcca ggctgatagc ccgggctgac
     1621 accgccagtg gaaatgatga tcttgtagta gggagtttcc cggatgacaa acccggtatc
     1681 cgcgcaatgc accagttttt gcggcccggt tttgtccgcc tctgtctcat tgccttgcag
     1741 ttttcgccag taggccgaca gcgttttcca gccgtgacag ccatggagcg tggaatcgcc
     1801 aaacagtgcc accttgtcat cggcaaagct caacagcttc aaaaaactca gaccgcgttg
     1861 aatgacggaa accaggcggg ccatgcaggg atgatccacg ccggcattgt gcagcagatt
     1921 gtaaaggtcc atacaatctt ccagacaaat ggcatggtac atgggagacc gctcatagtg
     1981 gccaccgtca tcgttgaact gctcattcag ttcgcgcaca agcagatcgc aataacgctt
     2041 cagccaggcc cggtaggttg gctcgctttg catgaacgca cagaaccaga tcatcgccac
     2101 tatattcttg aacagatgat tggcgcggat atggtactca atgttgcggt tgagccactc
     2161 gccctgctgc accaggctga acagccattc ttcgctgagg ctacctgcgg cctgctcgcg
     2221 agcgctgaaa tacttgatcc a
//
LOCUS       DASUEG010000024         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_263623, whole genome shotgun sequence.
ACCESSION   DASUEG010000024 DASUEG010000000
VERSION     DASUEG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263623"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFX11_00390"
     CDS             complement(<1..653)
                     /locus_tag="VFX11_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEX4937102.1"
                     /translation="MATINQLRSASLQERISCLLRASFCLVCLGCVDTRLMAQDLFAL
                     SLEELMMVTVEVASRQPEPLQYTPAAVTVFSREDIERMGVRWLDELVQYVPGAYPHQR
                     AGSAAPFWTTQFRGNPNENGSGLLLLIDGRRQNDAAFGTASTAWMRQSLAGFKRVEIL
                     RGPGSAIYGANATYAVINLITDPTQKAVTVAAGEHGATGVQLAAATSADDWQLSAKA"
     gene            complement(1089..>2594)
                     /locus_tag="VFX11_00395"
     CDS             complement(1089..>2594)
                     /locus_tag="VFX11_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX4937103.1"
                     /translation="AIRKSFTLLETVRGVRHTMADLLLQHDPKTYAMLQRGDSVGVFQ
                     VESRAQMAMLPRLKPEKFYDLVIQVAIVRPGPIQGDMVHPYLRRRQGLEPASYESEDI
                     KNVLERTLGIPIFQEQVIALAMVAAGFSGGEADQLRRAMAAWKRKGGLEPYEHKLAVG
                     MLERGYSPDFIQRIIAQIRGFGEYGFPESHAASFALLVYVSAWLKCHEPAAFCCGLLN
                     AQPMGFYSASQLVQDVKRHNIDVRPVDVLHSDWDHTLETDALQNHPQRQPALRLGLRL
                     VGNLSRTGADKILQKRAQKNFSSLADFTRRCRLQQKDMDALAHANALQSLSAHRFQAR
                     WDAQGLQQESPLLQQEQQRETLVELPAPTEAQNLLEDYHSTGLTLGRHPMALLRDHPA
                     LRGCKRYTDLPALGHKRFVRIAGIVTGRQRPGTATGVVFITLEDETGNSNIVVWSDLV
                     ERFRAEVLHARLLCVKGVVEREHNVIHVIAGHLQDMTELLGELDTQSRDFH"
BASE COUNT          525 a          776 c          763 g          530 t
ORIGIN      
        1 tgcgccttcg ccgatagctg ccagtcatcc gccgaggtgg cggcggccag ttgtacgccc
       61 gttgctccgt gctcaccggc cgccactgtc actgcctttt gggtaggatc ggtgatcagg
      121 ttaatcaccg cataggtggc attggcgccg tatatcgccg agcctggacc gcgcagaatc
      181 tccacccgct tgaatcccgc caggctctgt cgcatccacg cggtggaagc agtaccaaac
      241 gcggcgtcgt tctgccggcg cccgtcaatc agcagcaaca accctgaacc gttttcattg
      301 ggattgccgc ggaactgggt ggtccagaac ggcgcagcgg aaccggcgcg ctgatgggga
      361 taggcgccgg ggacgtattg caccagttcg tccagccagc gcacacccat gcgctcgata
      421 tcctcgcggg aaaacaccgt gacggccgct ggcgtgtact gcaagggttc cggctggcgc
      481 gaggcgactt ccacggtgac catcatcagt tcttccagcg acagcgcaaa cagatcctgt
      541 gccatcagcc gtgtgtcgac acagcctagg cacaccaggc agaagctggc gcgcaacagg
      601 cacgagatgc gttcttgcag tgacgcggag cgaagttggt tgatggttgc catgcgcgag
      661 cggatatcag ttgtcttcca ggtgaatgag ccagtacgtg agcgagccgg ggtgaaatgt
      721 gcgcgctgaa gtgcgcgttt ttttcatcca cggaagcaac actagcagca ggttctggaa
      781 ggcgccaaaa gcgtaaattg cggattttta tggataaaaa caggattttt agaacggggc
      841 ttttccattt taacgaagcg ttttccgcat cgcttgtaga aactgatcag catgaaaatt
      901 gtggttaatg agcccttggt accaacccgg ctttttcatt tgcgcaccgg gggtttggcg
      961 acaatggtgc gcaattgccg cacgtcggct ggcgcgggca taaaagcagt gttaccaatc
     1021 gcacataaat gcggggcata gatcagccaa tcatcaatgc aaaaaaaatc tgagcgccct
     1081 gcataaattc aatgaaaatc ccgcgactgc gtgtccagtt cgcccagcag ctctgtcatg
     1141 tcctgcaaat gaccggcgat gacgtgaatc acattgtgct cccgctccac cacgcccttc
     1201 acgcacaaca ggcgcgcatg caatacctcg gcacgaaagc gttccaccag atcgctccac
     1261 accacgatat tgctgttgcc ggtttcgtct tccagcgtga tgaagaccac accggtggcg
     1321 gtaccaggac gctggcggcc ggtaacaatg ccggcgatgc ggacgaagcg cttgtggccc
     1381 aacgcgggca gatcggtgta acgcttgcag ccacgcaacg cgggatgatc gcgcagcagc
     1441 gccatgggat ggcggcccag agtcaggccg gtgctgtgat aatcctccag caggttttgt
     1501 gcttcggtcg gcgccggcaa ttccaccagc gtttcccgtt gctgttcctg ttgcagcagc
     1561 ggtgattcct gctgcaatcc ctgcgcatcc cagcgcgcct gaaagcggtg cgcgctgagg
     1621 gattgcaggg cattggcatg ggcgagagca tccatgtcct tttgttgcag acggcaacgg
     1681 cgggtgaaat ccgccagtga actgaaattt ttctgtgccc gtttctgcag gattttgtcg
     1741 gcgccggtgc gtgacagatt gcccaccaga cgcaggccca gccggagcgc cggttgccgc
     1801 tgcggatggt tttgcagggc gtcggtttcc agcgtgtggt cccaatcgct gtgcagcaca
     1861 tccaccggcc gcacgtcgat gttgtgtcgc ttcacgtcct gcaccagttg cgaggcggaa
     1921 taaaatccca tgggctgggc gttgagcagg ccgcagcaaa acgccgccgg ctcatgacat
     1981 ttcagccagg ccgacacata caccagcagc gcaaaactgg cggcgtggga ttcaggaaag
     2041 ccgtattcac cgaaaccgcg gatctgcgca atgatgcgct ggatgaaatc cggcgaataa
     2101 ccgcgttcca gcatgccgac cgccagtttg tgttcgtaag gttccagtcc gcctttgcgt
     2161 ttccaggcgg ccatggcgcg gcgcagctga tccgcctcgc caccggaaaa acccgctgcc
     2221 accatcgcca gcgcgatcac ctgttcctgg aaaatcggaa tccccagcgt gcgttccagc
     2281 acatttttta tatcctctga ttcgtagctg gccggctcca atccctgtcg ccgccgcaga
     2341 tagggatgca ccatgtcacc ctggattgga ccgggccgca cgatcgccac ctggatcacc
     2401 agatcgtaaa atttttccgg cttcaggcgc ggcagcatcg ccatctgggc gcgggattcc
     2461 acctggaaca cgccgacgct gtcgccccgc tgtagcatcg cgtaggtttt cggatcgtgc
     2521 tgcagcagca ggtccgccat ggtgtgacgc acgccgcgca ctgtttccag caacgtgaaa
     2581 cttttgcgga tcgc
//
LOCUS       DASUEG010000025         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_286054, whole genome shotgun sequence.
ACCESSION   DASUEG010000025 DASUEG010000000
VERSION     DASUEG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286054"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFX11_00400"
     CDS             complement(<1..430)
                     /locus_tag="VFX11_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX4937104.1"
                     /translation="MNTSTFTFKKLSVSLLMAGALLVPAVQVSAAGPEPATTMDSVKT
                     NTKEGWKEGMIEGAYLFNSNLSALRIDVEVMGNTALLKGYVDSGAAKSLAEEIAYSVD
                     GIDKVNNQLTVDPNKAPAEPKTTSMLKSELSDAAITAKVKS"
     gene            complement(561..1289)
                     /locus_tag="VFX11_00405"
     CDS             complement(561..1289)
                     /locus_tag="VFX11_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4937105.1"
                     /translation="MHKGKILSVEDDKNLQLVISEYLQEDGYDVLLASNFSTAVEKIT
                     SERVNVVLLDLVLPDGDGLNLISTLKANSNAAIIVLSGKDNTMEKVVCLEMGADDYLT
                     KPFEMRELSARIKAVLRRVENLAQPARLETVAHPKWRLPNGWILDSLQYQLFDETARP
                     ADLTVGEFRLLSALTQAANRVLTREQLFEHARKGDCFDVYDRAIDTQIARLRKKMGDI
                     SKPPLLIKTIRGVGYMLCGSVTSA"
     gene            1481..1987
                     /locus_tag="VFX11_00410"
     CDS             1481..1987
                     /locus_tag="VFX11_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4937106.1"
                     /translation="MFSHHELVREMPNLRRFAIKLTRNNCDADDLLQSTMLKSLESRR
                     SFDRGSNLFGWTSRIMYNAFVSHYRRSVRFDSLYDPQHYIDQETVEPDQEITLELESV
                     LGAMARLSEDHREILSRVCLEGRRYAEVSELLQLPVGTVRSRLSRAREALVLALGQDP
                     EADARHAC"
BASE COUNT          452 a          569 c          618 g          501 t
ORIGIN      
        1 tggattttac cttggcagta atggccgcgt cggacagttc tgatttcagc atggatgtgg
       61 ttttcggttc ggccggtgcc ttgttggggt ccacggtcaa ctggttgttc accttgtcga
      121 taccgtccac actgtaggcg atttcttcag ccagcgattt ggcagcaccg gaatccacat
      181 agcctttgag cagcgcggtg ttgcccatga cttccacatc gatccgcagc gcactcaggt
      241 tggaattgaa cagataggcg ccttcgatca tgccttcttt ccagccttcc ttggtgttgg
      301 ttttcaccga atccatggtg gtggccggtt ccggcccggc agcgctcacc tgcacggccg
      361 gtaccagcag tgcacccgcc atcaagaggg aaaccgacag tttcttgaaa gtaaaggttg
      421 aagtgttcat ggtgtttgcc tcgcacgatg atgatgagtt gaagtgttcg ggtaacaaac
      481 gccgggcaga aaaaaaagtt ccggtagcaa ggggtgatcg aacggaacat aaatgctgat
      541 atatcttcac gtcagcgcga tcacgctgac gtaaccgatc cgcagagcat gtagcccaca
      601 ccgcgaatcg tctttatcag aagtgggggt ttgctgatgt cacccatctt tttgcgcagg
      661 cgggcaatct gggtgtcgat agcgcgatca taaacgtcga agcagtcgcc tttgcgcgca
      721 tgctcgaaca gctgctcacg ggtcagtacc cggttggctg cctgcgtcag ggccgacagc
      781 aaacggaatt caccgacagt cagatccgcc gggcgcgccg tttcgtcaaa gagttggtac
      841 tgcaggctgt caagaatcca gccgttgggc aatcgccatt tggggtgtgc caccgtctcc
      901 aggcgggccg gttgcgccag gttttccacc cggcgcaata cggccttgat acgcgccgag
      961 agttcgcgca tttcgaacgg tttggtcaga tagtcatcag cgcccatttc caggcagacc
     1021 actttttcca tcgtgttgtc cttgcccgac agcacaataa tcgctgcatt gctgttggct
     1081 ttgagtgtgc tgatgagatt caggccatcg ccgtcgggca gcaccaggtc gagcagtaca
     1141 acatttaccc gctctgatgt aattttttca acggccgtcg agaaattact ggccagcagc
     1201 acgtcatagc cgtcctcctg cagatactcg ctgatgacaa gctgcagatt tttatcatct
     1261 tcaacactca gaatcttgcc tttatgcatg ggaacgcatc cggaacaggg attgatgtca
     1321 tgactatgca aatgcaatgc acaaataacc ctgacgaacg tgtgctgaat tgaaacatgg
     1381 cagacacaaa ttgctatgtg gcggacatat ccgggagata aggcggctac gaacagggtt
     1441 tagcctttgc cgggtcaaac agcgataaag gagtttcgcc atgttcagtc atcacgagct
     1501 ggtacgggag atgcccaatc tccgccgttt tgccatcaag ctgacccgga ataattgcga
     1561 tgcggatgat ctactgcaaa gcaccatgct gaagtcgctg gaaagcaggc gcagtttcga
     1621 ccgcggctcc aatctgttcg gctggacgtc gcgcatcatg tacaacgcat ttgtgtccca
     1681 ttaccgtcgc agtgtgcgtt tcgattccct gtacgatccc cagcactaca ttgatcagga
     1741 gacggtcgag ccggatcagg aaatcacgct ggaattggaa tcggtgctgg gcgccatggc
     1801 ccggttgtca gaggatcacc gcgagatatt gtcccgggtg tgtctggaag gacgtcggta
     1861 tgccgaagta tcggagctgc tgcagttgcc agtgggcacc gtacgctcgc ggctttcgcg
     1921 cgcacgcgag gcattggtgc tggcgctggg gcaggacccg gaggcggatg cccggcatgc
     1981 ctgctgagaa cgggcgcagg atcaggccgt tgtgacggtt gcaccggtgt tcaggtattt
     2041 cagcatcacg cttttgagat ccgattccac aattggtttg ggcaggtagc tatccatgcc
     2101 ggcctggatg cagcggtcgc ggtcccccat cagggcgtgg
//
LOCUS       DASUEG010000026         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_285929, whole genome shotgun sequence.
ACCESSION   DASUEG010000026 DASUEG010000000
VERSION     DASUEG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285929"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFX11_00415"
     CDS             <1..663
                     /locus_tag="VFX11_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4937107.1"
                     /translation="EAAQEAIESVQVEPMGTVRLSCPTALLQYRISELVTRFMVEYPR
                     VNVVLEATNRRVDVIGERMDMALRVRFPPLEDSGLAMRTLVSSPQHLVVSPRFMENRC
                     MPQEPQDLVGLPSLDWGPPRDHSWCLESENGRSVELRHHPRYITDDMGSLRDAAIAGV
                     GVVHLPRMVVDADLAAGRLVSLLPGWAPRAGIVHAVFPSRRGRLPAVRALIDFLAENI
                     RL"
     gene            complement(747..835)
                     /locus_tag="VFX11_00420"
     tRNA            complement(747..835)
                     /locus_tag="VFX11_00420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(799..801),aa:Ser,seq:tga)
     gene            complement(871..2373)
                     /locus_tag="VFX11_00425"
     CDS             complement(871..2373)
                     /locus_tag="VFX11_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX4937108.1"
                     /translation="MSRAPNRTDISGNSVDTLPRPRLLAEQAFSRAAGAPLSGGNHLR
                     LLRDAAAHYPAWLNAIRSAQHSIHLENYIIENDRIGREFMAALAERARAGVQVRVLHD
                     WVGGFSANSPTLFRPLVEAGGEVRTFNPVRFDNPLSWLTRDHRKSLVIDGRLGYVTGV
                     CISARWLGDPARGIAPWRDTGIEISGPAVTDIAAAFAQAWATCGTPLPASELPVPGSL
                     PATGNTSLRVIASMPSTSGMYRLDQLIAAVASETLWLTDAYFVATPVYVQALCAAAQD
                     GVDVRLLVPSTSDIPLVSPLSRASYRPLLEAGVRVFEWNGSMIHAKTAVADGRWARVG
                     SSNLNLASWISNYELDVAVEDTAFAEEMEAMYLQDLENATEIILAGRNVKPVEPLQRR
                     RTPRAFREKGSGMTTAMRLGNTVASALRQQRVLGAAEAGLLAMLGVFLLLLAVVGLYW
                     PRLLAMPMAGISLWLAIWLLGRAWRLHRLHRPPPTITEPPKKSADVTPDG"
     gene            complement(2445..>2936)
                     /locus_tag="VFX11_00430"
     CDS             complement(2445..>2936)
                     /locus_tag="VFX11_00430"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX4937109.1"
                     /translation="VPDVMFPNVFDEADVGESALPNAMPWDTIPSARFFQYQDFGKQL
                     NAIRQASQARQADDPDFRYLNENISLARELKKQKTLSLKEAARKAEKQALDEKRLAME
                     NRRRAAKGQEPLKNWREAEQAEEEEQETPADPNEEKPEDEAFALEAGRILVDSLNPQL
                     GKR"
BASE COUNT          508 a          853 c          995 g          580 t
ORIGIN      
        1 gaggcggctc aggaggccat cgaatcggtg caggtcgagc cgatggggac ggtgaggctg
       61 tcctgcccga ctgccctgct gcaataccgt atcagcgaac tggtcacgcg cttcatggtg
      121 gaatacccgc gcgttaacgt ggtgctggaa gccaccaatc gacgcgtcga tgtcattggc
      181 gagcgtatgg acatggcact gcgtgtgcgc tttccgccgc ttgaggacag cgggctggcc
      241 atgcgcacac ttgtcagtag tccccagcat ctggtggtga gcccccgctt tatggaaaac
      301 cggtgtatgc cgcaagagcc gcaggatctg gttggattgc ccagcctgga ttgggggcct
      361 ccgcgtgatc acagttggtg tctggagagc gagaacggca ggtcggtcga gctgcgccat
      421 catccgcgct acatcaccga tgacatgggc tcgctgcgtg atgcggccat tgccggtgtc
      481 ggcgtggtgc acctgccccg catggtagtg gatgcagatc tggcggctgg acggctggta
      541 agtctcctgc cggggtgggc gcccagggca ggtatcgtcc acgcggtatt cccgtcgcgt
      601 cgagggcgtt tgccggctgt gcgtgcgctg atcgattttc tggcggaaaa catccgcctg
      661 taatctttgc ggaagcggtc agggaagtaa gggcgtctgc tggaacggga agatgaccgt
      721 gcaatggcca ggggtgctga agtgaatggc ggaagcggtg agattcgaac tcacggaggg
      781 cggaaccctc ggcggttttc aagaccgctg ccttcaacca ctcggccacg cttccacagg
      841 tcggcaagca tactggatga ggggtggcag tcaaccgtca ggcgtgacgt ctgctgattt
      901 tttgggcggt tcggtgatag tgggcggtgg gcgatgcagc cggtgcaatc gccacgcccg
      961 tcccaacagc cagatggcca gccacagact gatgccggcc atgggcattg ccagcagacg
     1021 cggccagtac agccccacga ccgccagcaa tagcaggaac acacccagca tggccagcag
     1081 gccggcctcg gcggcgccca gtacgcgttg ctgacgcagt gcactggcaa cggtgttccc
     1141 cagtcgcatg gccgtggtca tgcccgagcc cttttcacgg aaggcccggg gcgtgcgccg
     1201 ccgctgcagg ggctcgacgg gtttcacgtt gcggccggcc aggatgattt cggtcgcatt
     1261 ctccaggtcc tgcaggtaca tggcctccat ttcctcggca aatgccgtgt cctcgacggc
     1321 cacatccagt tcgtaattgc tgatccagct tgccaggtta aggttgctgg agccaacacg
     1381 tgcccagcga ccgtcggcca cagcggtctt ggcatggatc attgagccgt tccactcaaa
     1441 gacgcgcaca ccggcctcca gcagggggcg atagctggcg cgcgacaacg gtgacaccag
     1501 cggaatgtca ctcgtgcttg ggaccagcag gcgcacatcc acaccatcct gagcagcagc
     1561 acagagtgcc tgcacgtaga ccggagttgc cacgaaatag gcatcggtca gccacagggt
     1621 ttcgctggca acggcggcta tcagttggtc aagacggtac atgccgctgg tgctgggcat
     1681 gctggcaatc acgcgcaggc tggtgttgcc ggtcgcgggc aggctgccgg gaactgggag
     1741 ttcactggct ggtaacggcg taccgcacgt agcccaggct tgtgcaaatg ccgcggcaat
     1801 gtcggtgacg gcggggccgg agatttcaat gccggtatcg cgccacggtg ctatgccgcg
     1861 ggcagggtcg cccagccagc gggcgctgat gcagacgccg gtgacataac ccagccggcc
     1921 atcaatcacg agcgatttac gatggtctcg cgtgagccat gacagcgggt tgtcgaagcg
     1981 caccggattg aaggtgcgga cttcgccgcc ggcttctacc agcggccgga agagggtagg
     2041 tgagttggcg gaaaagccac ccacccagtc gtgcaagaca cgcacctgca caccggcgcg
     2101 ggcccgttca gccagcgcgg ccatgaattc gcgaccgatc cggtcgttct cgatgatgta
     2161 gttttcgaga tgaatgctgt gttgggcaga gcgaatggca ttcagccatg cggggtagtg
     2221 tgcggcagca tcgcgcagca ggcgcaaatg attgccgccg gacagcggtg caccggccgc
     2281 ccttgagaac gcctgttcgg ccagcagccg ggggcgtggc agggtgtcaa cgctgttgcc
     2341 ggaaatgtcg gtgcggtttg gagcccggga catgggtcat ttccattgat gcccggattg
     2401 atggtagcac cccggcctgc ctttggcagc gccggggtga ggtctcagcg tttgccgagc
     2461 tgcggattga ggctgtcgac cagaatgcgt ccggcttcca gggcaaaagc ttcgtcttcc
     2521 ggcttttcct cgttggggtc tgccggagtt tcctgctctt cttcttccgc ctgttcggct
     2581 tcacgccagt ttttcagtgg ctcctggccc ttggcagcac ggcggcggtt ttccatggcc
     2641 aggcgctttt cgtccagcgc ctgcttttcg gctttgcgtg ccgcttcttt cagcgacagg
     2701 gttttctgct tcttgagctc gcgggccaga gagatgttct cgttcaggta gcggaagtcg
     2761 ggatcatctg cctgacgggc ctgcgaggcc tggcgaatgg cattgagctg tttgccgaag
     2821 tcctggtact ggaagaagcg ggccgagggg atggtgtccc acggcatggc gtttggcagt
     2881 gcggattcgc cgacatcggc ttcgtcgaag acgttcggga acatcacgtc cggcac
//
LOCUS       DASUEG010000027         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1081441, whole genome shotgun sequence.
ACCESSION   DASUEG010000027 DASUEG010000000
VERSION     DASUEG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081441"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFX11_00435"
     CDS             <1..1121
                     /locus_tag="VFX11_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4937110.1"
                     /translation="HNEALVHIMIRLQFPVVSRYIEKPLAKLVRRRLAQHLAGVKTVA
                     DFQQLMVQFVTSVIQNSMTEFTFDGLDNLDPEESYVFISNHRDITLDSALLNYVLMQS
                     KRHSAEIAIGDNLLSNPLMSDLLRLNKSFIVKRSVPGLKAKLAALTELSGYISHSRAE
                     GNSIWIAQREGRAKDGCDRTDPAIIKMLHIYNKKQHIPFADSMRKLNIVPVAISYEYD
                     PCDVLKATEMQAREDASYVKREGEDVESILRGIALPKGRVHIQFGAPLQGEYHSADEV
                     AAAIDQAIVAGYRLFPSNLLAYEQLQKVREAVSSLQDAAAQMLVNIDTQELARKASEF
                     SARISSYPKRVQNLILEMYANPLLSKFQMQKDQPEQPA"
     gene            complement(1236..1805)
                     /locus_tag="VFX11_00440"
     CDS             complement(1236..1805)
                     /locus_tag="VFX11_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX4937111.1"
                     /translation="MRTLLSLLLALILFTPLAAPAKARQIGGIDLADSIQVGKQQLVL
                     NGAGVRNKFFMDVYVAGLYLSHASHDAATIVAADEVQSVRLVITSSQITRQRLLEAID
                     EGIHKSAGKDYPRYQPRMQELWDAITFEVKPGDIFAFTYIPGEGTHVSMNGKELRVLP
                     EFDFKKVLFGIWLGPDPIQASLKRDMLDE"
     gene            2008..>2110
                     /locus_tag="VFX11_00445"
     CDS             2008..>2110
                     /locus_tag="VFX11_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4937112.1"
                     /translation="MTGFDVVIVGGGAAGLMCAAQAARRGRKVVVLER"
BASE COUNT          439 a          597 c          635 g          439 t
ORIGIN      
        1 tgcataacga agcgctggtc cacattatga tccgcctgca gttccctgtg gtctcccgct
       61 atatcgaaaa gccgctggca aagctggtgc ggcggcgtct tgcccagcat ctggcgggtg
      121 ttaaaaccgt agccgacttc cagcagctga tggtccagtt cgtcaccagc gtgatccaga
      181 actcgatgac cgaattcacg ttcgacggcc tcgacaatct ggacccggaa gaatcttacg
      241 tcttcatcag caatcaccgc gacattaccc tggattcggc gctgctgaac tatgtgctga
      301 tgcagtccaa acgacattcg gccgaaatcg ccatcggcga taacctgctg agtaatccgt
      361 taatgtcgga tctgctgcgg ctgaacaaga gtttcatcgt caagcgctcg gtaccgggtc
      421 tcaaggccaa gctggcggcg ctgaccgaac tgagcggcta tatcagccat tcgcgtgcgg
      481 aaggcaattc catctggatc gcccagcgtg aaggccgcgc caaggatggt tgcgatcgca
      541 ccgatcccgc gatcatcaag atgctgcaca tctacaacaa gaagcagcac attcccttcg
      601 ccgactccat gcgcaaactg aacatcgtgc cggtggccat ttcgtacgag tacgatccgt
      661 gcgacgtgct gaaagccacc gagatgcagg cgcgggaaga cgcctcttac gtcaagcgcg
      721 aaggcgagga tgtggaaagc atcctgcgtg gtattgcgct gccgaaaggc cgcgtgcata
      781 tccagtttgg cgcgccgcta cagggtgagt atcactcggc agacgaggtg gccgccgcga
      841 tcgatcaggc cattgtggca ggttaccgcc tgtttccctc gaatctgctg gcatatgagc
      901 agctgcaaaa agtgcgggaa gcggtatcca gcctgcagga tgctgctgcg cagatgctgg
      961 tgaacatcga cacccaggaa ctggcgcgca aggccagcga gttttccgcc cgtatttcca
     1021 gttatcccaa acgggtgcag aacctgattc tggaaatgta tgccaatccc ctgctgagca
     1081 agttccagat gcagaaggac cagccggaac aaccggcctg aaccgcccgg gttgaccggc
     1141 gcgattggct ccaacagaaa tggccgcagt gatgcggcca tttttttccc ggctccccgc
     1201 gttcagtggg gccatgaaaa ctgcttgccg tacgcttact cgtccagcat gtcccgcttc
     1261 agtgacgcct ggatggggtc aggtcccagc cagatgccga acaacacttt cttgaaatcg
     1321 aattccggca gcacccgcag ttccttgccg ttcatgctca catgggtgcc ctcgcccggg
     1381 atgtaggtga aggcgaagat atcccccggt ttcacctcga aggtgatcgc atcccacagc
     1441 tcctgcatgc gcggctggta gcgcgggtag tccttgccag cggatttgtg aatgccttca
     1501 tcgatggctt ccagcagccg ctgacgcgtg atctgggacg atgtgatgac cagtcgtacc
     1561 gactgcactt catccgccgc gacaatggtg gcggcgtcgt ggctggcgtg ggacaggtag
     1621 aggcccgcca cgtaaacatc catgaagaac ttgttgcgca caccggcgcc gttcagcacc
     1681 agttgctgct ttccgacctg gatggaatcc gccagatcga tgccgccaat ctggcgtgcc
     1741 ttggctggcg cggccagcgg agtaaacagg atcagggcca gtaacaacga gagcagggta
     1801 cgcatggtgg actcccagac atggacttta atgcgcgtat cttaattgga ttgtcagaat
     1861 tgtaatttgc tccgctgcct gtgtgggtgg cgtcgcagcc aatgggcgcc ggcggtagtg
     1921 gggtacatgc tagaatgccg cggcccggcg gcggatgctg tggcgcgaaa cagggcgaaa
     1981 tattctgatt ttgaagggtt tttatctgtg acaggttttg acgtggtaat cgtgggcggt
     2041 ggtgcggccg gtttgatgtg tgccgcgcag gccgcccggc gcggcagaaa agtggtggta
     2101 ctggagcgta
//
LOCUS       DASUEG010000028         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_705600, whole genome shotgun sequence.
ACCESSION   DASUEG010000028 DASUEG010000000
VERSION     DASUEG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705600"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..1169
                     /locus_tag="VFX11_00450"
     CDS             6..1169
                     /locus_tag="VFX11_00450"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4937113.1"
                     /translation="MGKSFRGAFNDTEAPALGGHVVRAAVASAGIDPARIDDCIFGAA
                     AQQGTQAYNLGRLCAIAGGLPDSVAGMAIERQCSSGLMAIATAAKSIMCNEYDIAVAG
                     GVESISLTQNKFKNTYRSVSKTVTDIDPTAYIPMIETAEIVAQRYNISRAAQDEYSLL
                     SQLRTAAAQKAGIFEGEIVPMTVQKAIVNKETNETSYQTVTLSQDECNRADTTLASLQ
                     ALEPVWKDGKWVKQGQFITAGNASQLSDGASASVVMDGKLASQLGLNPLGAYRGLAVA
                     GCKADEMGIGPVFAVPKLLKRHGLKVDDIGLWELNEAFASQVIYCRDQLGIDNEKLNV
                     NGGAIAIGHPFGMSGARMVGHALLEGKRRKVKYVVVTMCIGGGMGAAGLFEVF"
     gene            complement(1268..>3115)
                     /locus_tag="VFX11_00455"
     CDS             complement(1268..>3115)
                     /locus_tag="VFX11_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937114.1"
                     /translation="FIRDLVITEPYVFLLRVGGSDNASGLNVLDVRNPHAPVLVKTLG
                     FSFLEIGNELERQGEFIYVVTDNRSSSDGTLRIVNVSDPLHPALVGSLPMEGDALSLV
                     VDGDYAYVVESHFGFPGGFNGVRVVDIRSPQQPALVASLELSAYPGKLVKSGSHLFVS
                     GYGGLSIVDVSNPLAPVLAGELPRRYGGAIAVAGDHVFLRHDDAGLDVVDVSNVTAPV
                     LLTTLNMDWANSDSTFGPIESILIEGNDAYIAFGGLGLHRVDISDPAAPRWAGSLDNP
                     DGINSAVALSQGLAYVATGTAGLRILDANALHRNPVLAGSLDLPGRLTAQTVANPLAA
                     YSPILGFGISIIDLWHPRAPELAATYNAETLQVSLAGRFAYLADFTAFHVLDIFNPWE
                     PVALPGTINMNERGFRYLIALDVSRDFAYVTGAAEGGSNTGRLAVIDIRDPQMPILSG
                     QLNFSGIPGEVVVQDDRAYVVVANSQLQVVDLQNPLQPLLAGSLNLPCTANFLAAADV
                     HVYVACGDGGMQVLDVSDLTAPILATTVDTAGSASTITLDGQFAYVADAVGGVVLIDV
                     ADPAAPVVIGNASVRGSARGAFVQNGLLYVSTSAGLDVLKKPRVAANSN"
BASE COUNT          702 a          980 c          882 g          551 t
ORIGIN      
        1 ccgcgctggg caaatcgttc cgtggcgcat tcaacgatac cgaagcgccc gcactgggtg
       61 gacacgtggt gcgtgcagcg gtcgccagtg ccggcatcga tcctgcccgc atcgacgatt
      121 gtattttcgg tgctgccgca cagcagggta cgcaggccta caatctcggc cgcctgtgcg
      181 cgattgccgg tgggctgccg gattccgtcg ccggcatggc catcgagcgc cagtgctcgt
      241 ccggattgat ggccatcgcc accgccgcca aaagcatcat gtgcaacgaa tacgatatcg
      301 ccgtcgccgg tggcgtcgaa tccatttcgc tgacccagaa caaattcaag aatacctacc
      361 gcagcgtgtc gaaaaccgtt accgatatcg atcccaccgc gtacattccg atgatcgaaa
      421 cggccgagat cgtggcgcag cgctacaaca tttcccgcgc cgcgcaggat gaatattccc
      481 tgctgagcca gctgcgcacc gctgccgcgc aaaaagccgg aatcttcgaa ggcgaaatcg
      541 tgccaatgac cgtgcagaag gccatcgtca acaaggaaac caacgagacc tcctaccaga
      601 ccgtcaccct cagccaggac gaatgcaacc gcgccgacac cacgctggcc agcctgcagg
      661 cgctcgaacc ggtgtggaaa gatggcaagt gggtgaaaca gggccagttc atcaccgccg
      721 gcaatgcgtc gcaattgtcg gatggtgcgt ccgcatcggt ggtgatggat ggcaagctgg
      781 catcgcagct gggcctgaat ccgctgggtg cttatcgcgg cctggcggtg gcgggatgca
      841 aggcggacga aatgggcatt ggcccggtgt ttgcggtgcc gaaactgctc aagcgtcacg
      901 gcctgaaagt ggatgacatc ggactgtggg aattgaatga agcctttgcg tcgcaggtga
      961 tctattgccg cgatcagttg ggaattgaca acgaaaaact caacgtcaac ggtggcgcca
     1021 tcgccatcgg ccacccgttc ggcatgagcg gcgcccgcat ggtgggccac gcactgctgg
     1081 aaggcaaacg gcgcaaggtg aaatacgtgg tggtcaccat gtgcatcggc ggtggcatgg
     1141 gtgccgccgg cctgttcgaa gtgttctgat cacaccggct ggagtgcagg gctaccatcc
     1201 cctgcgctcc aggtcattgg tctttttgcg gcgctttgta atcagaccgt ccaccaacaa
     1261 tgccacatca gtttgaattt gccgccacac gcggcttctt caacacgtcc agacccgcgc
     1321 tggtggaaac atacagcaac ccgttctgca cgaatgcgcc gcgcgcactg ccacgcacgg
     1381 atgcattgcc aatcaccacc ggggcagcag gatctgccac atcaatcagt acgacgccgc
     1441 cgacggcgtc cgccacatag gcgaattggc catccagagt gatggtgctg gcagaacctg
     1501 cagtatccac tgtcgtcgcc agtatgggcg cggtgagatc actcacatcc agtacctgca
     1561 taccgccgtc accacacgcc acatacacat gaacatctgc agctgccagg aaattggccg
     1621 tgcacggcag attcaggctg cccgccagca gcggttgcag cggattctgc agatccacca
     1681 cctgcagttg gctattcgcc accaccacgt aagcccgatc atcctgcacc acgacttcgc
     1741 cgggaatgcc ggaaaaattc agctgcccgg acaatatcgg catctgcgga tcgcggatat
     1801 cgatcaccgc cagtcgacca gtattgctgc cgccctcggc tgcacctgtg acataggcaa
     1861 aatcacggga cacatccagt gcaatgaggt agcggaagcc gcgctcattc atgttgatcg
     1921 tgcccggcag tgcaaccggt tcccagggat tgaaaatatc cagcacgtga aaagcggtaa
     1981 agtccgcaag ataggcaaag cgaccggcga gtgacacctg cagagtctcg gcattgtaag
     2041 tggccgccag ttccggtgcg cgcgggtgcc acagatcaat aatgctgatg ccaaatccca
     2101 ggatggggga atacgccgcc aacgggtttg ccacggtctg ggcggtcagg cgacccggca
     2161 agtccaggct gccggccaat accggatttc tgtgcagggc attcgcatcc agaatgcgca
     2221 aacctgcggt gccagtggcg acatacgcca gcccttgcga caacgccacg gcggaattga
     2281 tgccgtcagg attatccagc gatcctgccc agcgcggcgc tgccggatcg ctgatatcga
     2341 cgcggtgcag acccagcccg ccaaaggcaa tatacgcatc attaccctcg atcagaatgg
     2401 attcgatggg gccgaaggtt gaatcgctgt tcgcccagtc catattcagc gtcgtcaaca
     2461 gaactggcgc tgtcacgttg ctgacatcca ccacatccag gcccgcatcg tcatggcgca
     2521 gaaaaacgtg gtcaccggca acggcaatag cgccaccata cctgcgtggc aactcacccg
     2581 ccagcaccgg cgccagcgga ttgctgacat ccacgatgct cagcccgcca taaccgctga
     2641 cgaaaagatg cgagcccgac ttcaccaatt ttcccgggta ggcggacaat tccagactcg
     2701 ccaccagcgc gggctgctgc ggactgcgga tgtccaccac gcgcacaccg ttgaagccgc
     2761 cgggaaagcc aaaatgactt tccaccacgt aggcataatc gccatcaacc accagcgaca
     2821 gcgcatcgcc ctccatcggc aaactgccga ccagcgccgg atgcagcgga tcgctgacgt
     2881 tgacgattcg caaggtgcca tcgctggaac tgcggttgtc cgtcaccacg taaataaatt
     2941 cgccttgccg ttccagttcg ttgccgattt ccagaaaact gaaacccagg gttttcacca
     3001 gcacgggcgc atgcggattg cgcacatcaa gaacattcag gccactggca ttgtcgctgc
     3061 cacccacccg cagcagaaac acataaggct cggtgatgac cagatcccgg atgaa
//
LOCUS       DASUEG010000029         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1986902, whole genome shotgun sequence.
ACCESSION   DASUEG010000029 DASUEG010000000
VERSION     DASUEG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986902"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /gene="infB"
                     /locus_tag="VFX11_00460"
     CDS             <1..1628
                     /gene="infB"
                     /locus_tag="VFX11_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX4937115.1"
                     /translation="ETAQLIVEELGHKVKLVSADAVEDSLIESLGDTHQEGMPTARAP
                     VVTIMGHVDHGKTSLLDYIRRTRVAAGEAGGITQHIGAYHVDTGHGMITFLDTPGHAA
                     FTAMRARGAQCTDIVVLVVAADDGMMPQTAEAIDHARAAKVPLVVAVNKIDKPGADPE
                     RVRSELSQKEVISEEWGGDTQFVHVSALTGQGIDQLLDAILLQAEVLELQAVASGPAK
                     GVVIESSVDKGRGVVASLLVQSGTLNRGDMVLAGEHYGRVRAMLDENGRQIESAGPSI
                     PVEILGLAGAPDAGEEFLVVPDERKAREVADFRQKKLRHNRLSRQQATKLENIFETMG
                     AGDTASVNIVLKTDVRGSLEALQVALQDLSTPEVKVAIVSAGVGGINESDVNLALTSR
                     AVIIGFNVRADASARKLCQEEGVDLRYYSVIYDIIEDVKKAMSGLLEPELREEIVGVA
                     EVRDVFRSSKFGAVAGCKVMEGTVYRNKPIRVLRDNVVVFQGELESLRRFKDDVNEVN
                     AGIECGIAVKSYNDVKPGDKIEVFDVRKVARTI"
     gene            1640..2059
                     /gene="rbfA"
                     /locus_tag="VFX11_00465"
     CDS             1640..2059
                     /gene="rbfA"
                     /locus_tag="VFX11_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX4937116.1"
                     /translation="MRHHSRGFSRTDRIADQIQRDLALLLHREIKDPRVGLPTISSVK
                     VSRDLAYADVYITFMAIDEPEKVKQALSVLNNASGLLRSLLSKSIKLRVMPRLRFHYD
                     QTIVDGPRISALINAAVAEDKARHHDDGTDQTSPDGE"
     gene            2064..2990
                     /gene="truB"
                     /locus_tag="VFX11_00470"
     CDS             2064..2990
                     /gene="truB"
                     /locus_tag="VFX11_00470"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX4937117.1"
                     /translation="MAKRRGKRGRPVDGVLVLDKPQGCTSNEILQRVKRMYGADKAGH
                     TGSLDPLATGVLPLCFGEATKFSQYLLDADKAYVVKAKWGEKTNTADADGEVIETCAT
                     DNVSRERLQATLPQFMGEIEQVPSMYSALKHNGQPLYKLARQGVEIERQARKVTIHQL
                     DLLDCDAQHFSLFVRCSKGTYIRNLVEDIAQAMGNLSHVVELRRTQAGSYRLDQAVTV
                     EALVAALESGGHKALDELLLPLGTSADHWPTLQLSENTAFYLRNGHPVQVPGSPLEGW
                     VKLIGPAEEFIGVGEILDDGKVAPRRLINRSD"
     gene            3076..3345
                     /gene="rpsO"
                     /locus_tag="VFX11_00475"
     CDS             3076..3345
                     /gene="rpsO"
                     /locus_tag="VFX11_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX4937118.1"
                     /translation="MPISVQEKAKIVAEHQRAQGDTGSPEVQIALLTAQINQLQGHFK
                     AHEKDHHSRRGLIRMVNSRRKLLDYLKRKDQDRYTALIGKLGLRR"
     gene            3654..>4487
                     /locus_tag="VFX11_00480"
     CDS             3654..>4487
                     /locus_tag="VFX11_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX4937119.1"
                     /translation="MFKVVRKEFQFGNEKFVLETGKIARQATAAVMVTSGSCSVLVTV
                     VAKKDADPSKDFFPLTVNYQEKFYAAGRIPGSFHKREGRPSEKETLTSRLIDRPIRPL
                     FPEGFYNEVQVIATVMSTDKKTDPDIAAMLGASAALAISGIPFMGPIGAARVGYRDGM
                     YILNPNYKDLETSQLELVVAGTDEAVLMVESEAQELTEDQMLGAVLFGHLEMQVAVNA
                     IKEFAKEAAKAPMAWQPPAENTVLKEALAAYAAQVTEAYLIRDKQARYSRIGEIKSAA
                     IA"
BASE COUNT          970 a         1287 c         1376 g          854 t
ORIGIN      
        1 gtgaaacggc ccagctgatt gttgaagagt tgggccacaa ggtgaagctg gtgagtgctg
       61 acgctgtcga ggactcgctt atcgaatccc tgggcgacac ccatcaggaa ggcatgccga
      121 cagcccgtgc gccagtcgtc accatcatgg gccacgtcga ccacggtaaa acttcgctgc
      181 tcgactatat ccgccgcacc cgtgtggcgg ctggtgaagc cggcggcatt acccagcaca
      241 tcggcgccta tcatgtggat accggtcacg gcatgatcac gttcctggat accccgggtc
      301 acgccgcgtt taccgccatg cgtgcccgtg gtgcccagtg caccgacatc gtggtgctgg
      361 tggtagccgc agatgacggc atgatgccgc agactgccga agcgattgat cacgcccgtg
      421 ccgcaaaagt gccgctggtc gtggccgtga acaagatcga caaacccggt gcagatcccg
      481 agcgggtccg ttccgagctg tcgcagaagg aagttatttc cgaagagtgg ggcggcgaca
      541 cccagttcgt gcatgtgtct gcgctgaccg gtcagggcat cgaccagttg ctggatgcca
      601 tcctgctgca ggcagaagtt ctcgaactgc aggcggtggc ctccggtccg gccaagggtg
      661 tggtgatcga atcctccgtc gacaagggtc gcggtgtggt ggccagcctg ctggtgcagt
      721 ccggtaccct gaaccgtggc gacatggtgc tggcgggcga acattacgga cgtgtccgcg
      781 ccatgctgga cgagaacggc cgccagatcg aaagtgctgg tccgtccatt ccggtggaaa
      841 tcctgggtct ggccggagcg ccggatgcgg gcgaggaatt cctggtggtg cctgacgagc
      901 gcaaggcgcg cgaagtggcg gatttccgcc agaagaaact gcgccacaac cgcctgtctc
      961 gccagcaggc gaccaagctg gaaaatattt ttgaaaccat gggtgcgggt gataccgcct
     1021 cggtcaacat tgtattgaag accgacgttc gcggctcgct ggaagcgctg caggttgctc
     1081 tgcaggatct gtcgactccc gaagtcaagg tcgcaatcgt gtctgccggc gtgggtggca
     1141 tcaacgaatc cgatgtcaac ctggcgctga cttcccgcgc agtcatcatc ggctttaacg
     1201 tgcgtgcaga cgcgtcagca cgcaagctgt gtcaggaaga aggtgtcgat ctgcgctatt
     1261 acagcgtcat ctacgacatc atcgaagacg tgaagaaggc gatgtccggt ctgctcgagc
     1321 ccgagctgcg cgaggaaatc gtgggcgtgg cagaagtgcg cgatgtgttc cggtcctcga
     1381 agttcggcgc agttgccggc tgcaaggtca tggaaggcac tgtttaccgc aacaagccga
     1441 tccgggtact gcgtgacaac gtggtggtgt tccagggcga actcgaatcc ctgcgtcgct
     1501 tcaaggatga cgtcaacgaa gtcaacgccg gtatcgaatg cggtatcgcg gtgaagagct
     1561 acaacgacgt caagcctggc gacaagatcg aagtgttcga cgtcaggaaa gtggcccgga
     1621 ccatttgagg taagccgact tgcgtcatca ttcccgcggc ttcagccgca cggaccggat
     1681 tgccgaccag atccagcgtg accttgctct cctgttgcat cgggagatca aggatccgcg
     1741 ggttggcctg ccgaccatca gcagtgtgaa ggtgagccgc gatctggcct atgccgacgt
     1801 gtacatcacc ttcatggcca tcgatgaacc agagaaagtc aaacaggcgc tgagcgtgct
     1861 caacaacgcc agtggcctgc tgcgttcgct gctgtcgaaa agcatcaagc tgcgcgtcat
     1921 gccccgcctg cggtttcact acgaccagac catcgtcgat ggcccccgca tctcggccct
     1981 gatcaacgcc gccgtggcgg aagacaaggc ccgccatcac gatgatggca ccgaccagac
     2041 cagccccgac ggagagtaac ccagtggcaa aacgtcgcgg aaaacgtggc cgtccggtgg
     2101 acggcgtgct ggtgctggac aagccgcaag gctgcacctc caatgaaatc ctgcaacggg
     2161 tcaagcgcat gtacggcgcc gacaaggccg gccataccgg cagtctggac ccgctggcca
     2221 ccggtgtgtt gccgctgtgt tttggcgagg ccaccaagtt ctcccagtac ttgctggatg
     2281 cggacaaggc ctatgtggtg aaggcgaagt ggggcgagaa aaccaacact gccgatgccg
     2341 acggtgaggt tatcgagacc tgtgccaccg acaatgtcag ccgcgagcgt ctgcaggcga
     2401 ccctgccgca gttcatgggc gagatcgaac aggtgccgtc catgtattcc gccctcaagc
     2461 acaatggcca gccgctgtac aaactggcgc gccagggcgt tgaaattgaa aggcaagccc
     2521 gcaaggtcac catccatcag ctggacctgc tagactgtga cgcgcagcat ttcagcctgt
     2581 ttgtgcgctg cagcaagggc acctacatcc gcaatctggt ggaagatatc gcccaggcga
     2641 tgggaaatct gtcgcatgtg gtagaattgc gccgcactca ggcgggctct tatcggctgg
     2701 atcaggcggt caccgtggaa gccctggtgg cagcactgga aagcggcggc cacaaggcgc
     2761 tggatgagct gttgttgccc ctgggcacca gcgcagacca ctggccaacg ctgcaactgt
     2821 ctgagaatac tgcattttat ttacgcaatg gccaccccgt gcaggtaccg ggatcgccgt
     2881 tggaaggctg ggtcaaactg attggcccgg cagaagaatt tatcggggtg ggcgagatac
     2941 tggacgatgg aaaagtagct ccgcgccgcc tgataaatcg ctctgactag aggccagggc
     3001 gccaagcaag accgcagcaa ctgtaaatat gcgtggttgc agcggttttt cccaaaagca
     3061 tattggagca agacaatgcc aattagtgtg caagaaaaag caaagatcgt tgccgaacac
     3121 cagcgtgccc agggcgacac cggttcaccg gaagtgcaga ttgctctgct gaccgcccag
     3181 atcaaccagc tgcaaggaca tttcaaggcc cacgaaaagg atcaccattc ccgtcgtggc
     3241 ctgattcgca tggttaactc gcgccgcaag ttgctcgact acctgaagcg caaggatcag
     3301 gaccgttaca ccgccctgat cggcaagctt ggcctgcgtc gttgatgatc gattggctaa
     3361 acctgattct gccagccagc tctgccgcat gtccccgggc atgttgtggg gctggtttct
     3421 gaatcaagct ggaatacggc acttgttccc cggaacaggt gccgtgtttt tttctgttgt
     3481 tggaaaacgg gtcgggtgca tctaacagcc ggttgccgcc agggcggaac cagccggttg
     3541 ctggatgtac ccaacaacga acacataaat agcgtcccgg ttgtcatggc agctgacccc
     3601 tgatgagcca tggcaggaat tggccgggac tttgaactgg aaggtaaata aaaatgttta
     3661 aagtcgtaag aaaagaattt caattcggta atgaaaaatt cgtgctggaa accggcaaga
     3721 tcgcccgcca ggccacagcg gccgtgatgg ttacatcagg ttcctgttct gtgctggtca
     3781 ccgtggtggc caagaaggac gctgatccgt ccaaggattt cttccccctc accgtcaact
     3841 atcaggaaaa attctatgcc gccggccgta ttcccggcag cttccacaag cgtgaaggcc
     3901 gtccgtccga gaaggaaacc ctcacctcgc gcctgatcga ccgtccgatc cgtccgctgt
     3961 ttcctgaagg cttctacaac gaagtgcagg tgattgccac ggtcatgtcc accgacaaga
     4021 agaccgatcc ggatatcgcg gcgatgctcg gtgcctccgc ggcgctggcc atttccggta
     4081 tccccttcat ggggcccatc ggtgccgccc gcgtcggtta ccgtgacggc atgtacatcc
     4141 tgaatccgaa ctacaaggat ctggaaacct cgcagctgga gctggtggtt gccggtacgg
     4201 atgaagccgt actgatggtg gaatccgaag cgcaggaact gaccgaggac cagatgctgg
     4261 gcgcggtgct gttcggtcac ctggaaatgc aggtggcggt caacgccatc aaggaattcg
     4321 cgaaagaagc tgccaaggcg ccgatggcat ggcagccgcc ggcagaaaac actgtgctga
     4381 aggaagccct ggctgcctat gccgcgcagg taaccgaagc ctacctgatc cgcgacaagc
     4441 aggcccgcta cagccgtatt ggcgagatca agtcggcggc catcgct
//
LOCUS       DASUEG010000030         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2075277, whole genome shotgun sequence.
ACCESSION   DASUEG010000030 DASUEG010000000
VERSION     DASUEG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075277"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFX11_00485"
     CDS             <1..259
                     /locus_tag="VFX11_00485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX4937120.1"
                     /translation="CEGATAERKWILHTEELPVNPFTGEAPQSVCVAIGPEGGFSDEE
                     VTQAKEQGFSVITLGPRIWRTETAPVVILSLIQQAWGDFSL"
     gene            341..1672
                     /locus_tag="VFX11_00490"
     CDS             341..1672
                     /locus_tag="VFX11_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4937121.1"
                     /translation="MDDPILQSLLQALAVSPDNLPLRLMIVRHCLKADRAADALEHAR
                     ALQPEQLDENEDRLLLAQLYLDQNQPQAALPFCTGTLPRQALLRARALLALGQTAQAQ
                     DAYRAAIGQDKSLEDAAFARQLGIQEGDGRPKLRVVANDNTNVTEIRRLLAPEEAPIR
                     FADVGGLEDVKKQIHKRIILPFQKPSLFQRFRKRVGGGILLYGPPGCGKTLLARATAG
                     ECNARFLNVELSDILDMYIGESEKKLHAIFELARQQAPAVIFFDEVEALGGKRQYSRE
                     STSSKLVSQFLSELDGFSRNNEGVLILASTNVPWALDSAFLRPGRFDRLVFIPPPDLP
                     ARRSILQLLLQGRPVEDRIDLDFLARNSGGFSGADLKNLVETAIDLAIEATLQEGSEV
                     PVSDAHLKEALREVRPTTLEWLTSARNYAKYANESGLYNDVLDFLNKHGKG"
     gene            1676..2152
                     /locus_tag="VFX11_00495"
     CDS             1676..2152
                     /locus_tag="VFX11_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937122.1"
                     /translation="MATDYALPDEPKPGTLAQFALNPMWPMFTLMLIGSGAAWLWFLF
                     NSIAQGSPFRRQEMLWIGGGVLLSFAYVFGLFTLVNNGTVPKIAIPYCLIGLTVVKLV
                     VGYRVYLYQVRSFELFQYYSSTPVKNGALPFMLLWFVAGRLKLHEQLPVLVTLALG"
BASE COUNT          414 a          665 c          709 g          453 t
ORIGIN      
        1 gtgcgaaggc gccactgccg aacgcaaatg gattctgcac acggaagaac tgccggtgaa
       61 tccgtttacc ggcgaggcgc cgcaatcggt gtgtgtcgcc atcggcccgg aaggcggttt
      121 cagcgatgag gaagtgacgc aggcgaagga gcagggattc agcgtgatca ccctgggccc
      181 ccgcatctgg cgcaccgaga cggcgccggt ggtgattctg agcctgatcc agcaggcgtg
      241 gggggacttt tccctgtgac cataatcaag accgtgtcac cagactgccc tgccggccct
      301 gtatttttgc aattccaatt tttctgccag gtatcaatcc atggatgatc ccattctgca
      361 atccctgctg caggcgctgg cggtgtcgcc ggacaatctg ccgttgcgcc tgatgattgt
      421 acgccactgt ctgaaggccg accgtgccgc cgacgcgctg gaacatgccc gcgccctgca
      481 gccggaacaa ctggacgaga acgaggatcg tctgctgctg gcgcagctgt atctggacca
      541 gaaccagccg caggcagcct tgccgttctg caccggcacg ctgccacggc aggccctgct
      601 gcgggcgcgt gcgttgctgg cactggggca gactgcccag gcccaggatg cctaccgtgc
      661 tgccatcggc caggacaaat ccctggagga tgcggcgttc gcacgccagc tcggtattca
      721 ggagggcgac ggccgcccca aattgcgggt ggtggccaac gacaacacca acgtcaccga
      781 aatccgccgt ctgctggcac cggaggaagc gccgatccgc ttcgccgatg tgggcgggct
      841 ggaggatgtc aaaaaacaga ttcacaagcg catcatcctg ccgttccaga aaccgtcgct
      901 gttccagcgt tttcgcaagc gcgtcggcgg cggtatcctg ctgtacggtc cgcctggttg
      961 tggcaagacg ctgctggccc gcgccaccgc cggcgaatgc aatgcacgct ttttgaatgt
     1021 ggagctgtcc gacattctcg acatgtacat cggcgagtcc gagaagaaac tgcacgcgat
     1081 tttcgagctg gcgcggcagc aggcaccggc ggtgattttt ttcgacgaag tggaagcgct
     1141 gggtggcaag cgccagtaca gccgcgagtc cacgtcgtcc aaactggtga gccagtttct
     1201 gtcggagctg gatggttttt cccgcaataa cgaaggcgtg ctgatcctgg cgtccaccaa
     1261 cgtgccctgg gcgctggact cggcattttt gcgccccggc cgtttcgacc gtctcgtatt
     1321 tattccgcca ccggatctgc cggcgcggcg ttcgattctg caattgctgc tgcagggccg
     1381 gccggtggag gatcgcatcg atctggattt tctcgcccgc aacagcggcg gtttttccgg
     1441 tgccgatctg aagaatctgg tggagacggc gatcgatctg gccatcgaag cgacgctgca
     1501 ggaaggcagc gaagtgccgg tgtcggacgc gcatctgaaa gaagcattgc gggaagtgcg
     1561 ccccaccaca ctggaatggc tcaccagtgc gcgcaattac gcgaaatatg ccaacgaatc
     1621 cggcctgtac aacgacgtgc tggattttct caacaaacac ggaaagggct gagccatggc
     1681 gactgattac gcgttgccgg acgaacccaa gcccggcacc ctggcgcaat tcgcgctgaa
     1741 tccgatgtgg cccatgttca cgctgatgct gatcggcagc ggtgccgcct ggctctggtt
     1801 tttgttcaac agcattgccc agggcagccc gttccggcgt caggaaatgc tctggatcgg
     1861 tggcggtgtg ctgttgtcgt ttgcttatgt gtttgggctg ttcacgctgg tcaacaatgg
     1921 caccgtgccc aaaatcgcga ttccgtattg tctgatcggg ctgacggtgg tgaagctggt
     1981 ggtgggttac cgcgtgtacc tgtaccaggt gcgcagtttc gagctgttcc agtattacag
     2041 ttccacgccg gtgaagaatg gcgcgctgcc tttcatgctg ctgtggttcg tggcgggccg
     2101 gctcaagctg cacgagcagt tgccggtgct ggtcaccctg gcgctgggtt gaggaagcga
     2161 cgatggataa cggattcctg caacagctgc tcgcccacgc cgaacaccag ctgcgccacg
     2221 gcaaccggca tggcgccatc a
//
LOCUS       DASUEG010000031         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1345733, whole genome shotgun sequence.
ACCESSION   DASUEG010000031 DASUEG010000000
VERSION     DASUEG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345733"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFX11_00500"
     CDS             <1..383
                     /locus_tag="VFX11_00500"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX4937123.1"
                     /translation="PAAHIAVGRALASLRERNVLIVGSGMSFHNMQAFFHTAASEQGR
                     WAREFDKWLLATCCDPQLPVIEREQRLAHWEQAPHARYNHPREEHLLPLQVCFGAASV
                     ASPVAEAVFHGEVLGKTVAAFLWR"
     gene            559..786
                     /locus_tag="VFX11_00505"
     CDS             559..786
                     /locus_tag="VFX11_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEX4937124.1"
                     /translation="MKRVALTVLLLSVSTASFAFKPCDELKSEIQTKLDAKGVVNYAL
                     DVVAAADVGEQKVVGSCEGGSKKIVYSRTQP"
     gene            complement(855..>2193)
                     /locus_tag="VFX11_00510"
     CDS             complement(855..>2193)
                     /locus_tag="VFX11_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HEX4937125.1"
                     /translation="ELDDVFELPYQRIPHPVYGDARIPAYEMIRFSVNIMRGCFGGCS
                     FCSITEHEGRVIQSRSEDSIIREIESIRDKTPGFTGTISDLGGPTANMYQLNCVSEEI
                     HANCRRLSCVFPTICKNLVTDQSPTTRLYRRARALPGVKRVMVASGLRYDLAVLDPEY
                     VRELVTHHVGGYLKIAPEHTENGPLSKMMKPGMGAYDEFKKMFDQFSKEAGKEQFLIP
                     YFIAAHPGTTDEDMMSLAFWLKNNGFRADQVQTFYPSPMAMATTMYHTQKDPIHRVNY
                     KSDKVFIPKGLKQRRLHKAFLRYHDPDNWPMIREALQRMGKAHLIGFGKKHLVPPTQP
                     ANHPSAQTGAEGYKRKPAPQGDQPKRGEKSVRGEKVMRTEKAVRGERTGRSDQPRRGQ
                     ILTQHTGLPPRQGTNAKPVGKRGEKLAPASAGKRAPGAALKRGPGHPKGKKPR"
BASE COUNT          386 a          644 c          658 g          505 t
ORIGIN      
        1 atcccgccgc tcatattgct gtgggccgcg ccctggccag cctgcgtgaa cgcaatgtgc
       61 tgatcgtggg atccggcatg tcgttccaca acatgcaggc gtttttccac accgccgcgt
      121 ccgagcaggg acgctgggcg cgggaattcg ataagtggct gctggccacc tgctgcgatc
      181 cgcagttgcc agtgatcgag cgcgagcaac gccttgcgca ctgggagcag gcaccccatg
      241 cccgctacaa ccatccccgc gaagagcatc tgctgcccct gcaggtgtgt ttcggcgcgg
      301 catcggtggc atcccccgtg gcggaagccg tgttccatgg tgaggtgctg ggcaagacgg
      361 tcgctgcgtt tttatggcgc tgatgtgccc tgtcttcagg ctccgccgcc cacacatcac
      421 aattcgtcat aatcgtaccg gtatcctgcc gcattcccct tgcacactat cctcattcct
      481 ggcggtgatg gtttgaagga gaatgagcgc ctcactccat acctccaggc tcccactaca
      541 tttaaacgga gaattgtcat gaaacgcgtt gccctgactg tcctgctgct gtccgtatcc
      601 actgcttctt ttgctttcaa gccctgtgac gaactgaaat cggaaatcca gaccaagctg
      661 gatgcgaaag gcgtggtgaa ttacgcgctg gacgtggtgg ctgcagccga tgtgggcgag
      721 cagaaagtgg tgggcagttg cgagggtggc agcaagaaga tcgtgtacag ccgcacccag
      781 ccgtaagggt tgcggacaaa caaaaagggc ggattgcctg atgcactccg ccctttttgt
      841 ttttcgacgg caggttagcg cggtttcttc cctttcggat ggccagggcc gcgtttcagc
      901 gcagcgccgg gcgcgcgttt gcctgccgac gccggagcca gtttttcgcc ccgtttacca
      961 accggttttg cgttggtgcc ctgccgtggt ggcaggccgg tatgctgcgt caggatctgg
     1021 ccgcggcggg gttgatcgct gcgtccggtt cgttccccac gcaccgcttt ttctgtgcgc
     1081 atgacttttt ccccacgcac gcttttttcg ccacgtttgg gctggtcgcc ctgtggtgca
     1141 ggcttgcgct tgtaaccctc cgcaccggtt tgtgccgacg gatgattggc cggctgtgtc
     1201 ggcggcacca gatgtttctt gccgaacccg atcaggtgcg ccttgcccat gcgttgcagt
     1261 gcttcacgga tcatcggcca gttgtccgga tcgtgatagc gtaaaaacgc cttgtgcagc
     1321 cggcgctgtt tcagaccttt cggaatgaac accttatctg acttgtaatt cacccggtga
     1381 atcggatctt tctgggtgtg gtacatggtg gtggccatgg ccatcggcga cggatagaac
     1441 gtctgcacct gatcggcgcg gaaaccgttg ttcttcagcc agaacgccag gctcatcatg
     1501 tcttcatcgg tggtgcccgg atgcgccgcg atgaaatacg gaatcaggaa ctgttccttg
     1561 cccgcttcct tcgaaaactg gtcgaacatt ttcttgaatt catcgtacgc gcccatgccc
     1621 ggtttcatca ttttcgacag cgggccgttt tcggtatgct ccggcgcgat tttcagataa
     1681 ccgcccacat gatgtgtcac cagctcgcgc acgtattccg gatcgagcac tgccagatcg
     1741 tagcgcaggc ccgatgccac catcacccgt ttcacgccgg gcagggcgcg ggcacggcga
     1801 tacaggcggg tcgtcggcga ctgatcggtg acaaggttct tgcagatggt gggaaacacg
     1861 caggacaggc ggcggcaatt ggcgtggatt tcctcgctca cgcaattgag ctggtacatg
     1921 ttcgcggtgg ggccgcccag atcggaaatg gtgccggtga agcccggtgt cttgtcgcgg
     1981 atgctttcga tctcgcggat gatggaatct tccgaccggc tctggatcac gcggccttcg
     2041 tgttcggtga tggaacagaa cgagcagccg ccgaaacagc cgcgcatgat gttcaccgaa
     2101 aagcggatca tttcgtatgc cggaatgcgc gcgtcgccat acactggatg cgggatgcgc
     2161 tggtacggca actcaaatac atcatccagt tcc
//
LOCUS       DASUEG010000032         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1434443, whole genome shotgun sequence.
ACCESSION   DASUEG010000032 DASUEG010000000
VERSION     DASUEG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434443"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VFX11_00515"
     CDS             <1..1315
                     /locus_tag="VFX11_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4937126.1"
                     /translation="VASPEMARDFFNLKPEQIDQRQLMIPIDIGDNQTGFLNNKEHQD
                     SRKIMIPPLVATRLRDRAAVMHELTTRHINAMKPGDTFDMPRLIGDITLDIACYTLLG
                     LKEGPKLQRYRELMLAWIGASTNDTMFTLGNLWGAPRFRSMLHRAYLKKTAKKDFGKG
                     KKGWLPWSRSVELKAQLADLMRQEIHAVRASGDTTRTDLIATLSRATYDDGELLPEER
                     IISESMGMLVGGHETSAATGAWFMLWLLKRPDVMQKMREEVLACVAQEGHFDPLKIVE
                     LPYLNACLNESQRLTPSAVGTMRHLVGETKIGPLTLPAKSNVLAGAYLIHRRKDVWGE
                     DAEEFKPERWLAGIKPGPFEFFPFGGGRRACIGSNQARQQLRILFAEFARRVEFTSKF
                     ANNDVWPGQRQVSGQTEPEGGVPVTVKKVWPENTGYPVPAVAQSA"
     gene            complement(1398..2135)
                     /locus_tag="VFX11_00520"
     CDS             complement(1398..2135)
                     /locus_tag="VFX11_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937127.1"
                     /translation="MKLRQRSILITGGTSGIGAALVSQLAGVNAEIVVIGRNPDKLDR
                     LRQDYSNVTPYRCALDSRQAVERTIETILARHPSLSVVINNAAVQYTPTLLDAEFSFA
                     SIENEITTNLTAPIWISALLLGHFQALQQNAAVINITTGLALFPKTTSAVYCATKAGL
                     HFFSRSLRYQLEATRISVHEAIMPLVDTAMTADRDGGKMTAPAAAAAIIRGVERDRAE
                     FHVGKARLIPVIARISPALMGRIMKTG"
     gene            2231..>2542
                     /locus_tag="VFX11_00525"
     CDS             2231..>2542
                     /locus_tag="VFX11_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4937128.1"
                     /translation="MELNNVFADASAKQRKVPKGQHVFRQGEKTDRICLIQQGLIKAY
                     YETRDGKEFIKSFIAEGGIIASLQAIVERQPSTFSVLALEDCIILELPKSVLDARQAQ
                     DP"
BASE COUNT          545 a          767 c          756 g          474 t
ORIGIN      
        1 ggtggccagt cctgaaatgg cgcgggattt tttcaacctg aaaccggaac agatcgacca
       61 gcgccagttg atgattccca tcgacatcgg cgacaaccag accggctttc tcaacaacaa
      121 ggaacaccag gattcgcgca agatcatgat tccgccgctg gtggccaccc gcctgcgcga
      181 ccgcgcggca gtgatgcatg aactcaccac gcgccacatc aatgcgatga aacccggcga
      241 cacgttcgac atgccgcgcc tgatcggcga catcaccctg gatatcgcct gctacaccct
      301 gctgggcctg aaggaaggcc ccaagctgca gcgttaccgc gaactgatgc tggcatggat
      361 cggcgcatcc accaacgaca ccatgttcac gctgggcaac ctttggggcg cgccgcgttt
      421 ccgcagcatg ctgcaccgcg cctatctgaa gaaaaccgcg aaaaaggatt tcggcaaagg
      481 caaaaaaggc tggctgccct ggagccgctc ggtggagctg aaagcgcagc tggctgacct
      541 gatgcggcag gaaatccacg ccgtgcgcgc cagtggcgac accacgcgca ccgatctgat
      601 tgccaccctg tcccgcgcca cctatgacga cggcgaactg ctgccggaag aacgcatcat
      661 ttccgaaagc atgggcatgc tggtgggcgg acacgaaacc agtgccgcca ccggcgcctg
      721 gttcatgctg tggttattga agcggccgga tgtgatgcag aagatgcgcg aggaagtgct
      781 ggcgtgtgtc gcgcaggaag gccacttcga tccactgaaa atcgtcgagc tgccatatct
      841 gaatgcgtgc ctgaatgaat cgcagcgcct gacaccttcc gccgtgggca ccatgcgcca
      901 cctggtgggt gaaaccaaaa tcggcccgct gaccctgccg gccaaatcca acgtactggc
      961 gggcgcttac ctgattcacc gccgcaagga cgtgtggggc gaagacgcgg aagaattcaa
     1021 accggaacgc tggctggccg gcatcaagcc cggaccgttc gaatttttcc cgttcggcgg
     1081 cggccgtcgc gcctgtatcg gttccaatca ggcacgccag cagttgcgca ttctgttcgc
     1141 agaatttgca cggcgggtgg agttcacgtc aaagttcgcc aacaacgatg tatggccagg
     1201 ccagcgccag gtcagtggcc agaccgaacc ggaaggcggc gtgccggtga ccgtgaagaa
     1261 agtgtggccg gaaaacacgg gctatccggt gccggcggtg gcacagtccg cctgataggc
     1321 tgttaatcgc caagcggctg cagcgccggc ccgatttccg gtaaggggat cggccggggc
     1381 tgccgccagt gtccagatca gcctgtcttc atgatccgcc ccatcagcgc agggcttatc
     1441 ctggcaatga ccggaatcag gcgagccttg ccgacatgga attccgccct gtcccgttca
     1501 acgccgcgaa tgatggccgc tgccgctgcc ggcgccgtca tcttgccacc gtccctgtct
     1561 gccgtcatgg ccgtgtccac caggggcatg atggcctcgt gcacactgat cctcgtcgct
     1621 tccagctgat agcgcaggct gcgggaaaaa aagtgcagac ccgctttcgt cgcgcagtaa
     1681 acggccgatg tggttttcgg aaacagtgcc agacctgtcg tgatgttgat gaccgcggca
     1741 ttctgctgca acgcctgaaa atgccccagc agcagggcac tgatccagat cggcgccgtc
     1801 agattggtgg taatttcgtt ttcgatgctg gcgaagctga actcagcatc gagcaaggtg
     1861 ggcgtgtact gcacagccgc attgttgata accaccgaca gggaaggatg cctggcgaga
     1921 atcgtttcga tagtgcgttc caccgcctgc cgcgaatcca gcgcacatcg gtaaggcgta
     1981 acgttggaat aatcctggcg caaacggtca agcttgtccg gattccgtcc gatcaccacg
     2041 atttccgcat tgacaccggc cagctgactg accagtgccg ctccgatacc cgacgtgcca
     2101 ccggtaatca gtatcgatct ttgtcgcagt ttcatctacg ggtccactcc acgttggggc
     2161 ctttgcgcta cacgatagcg gtcccctaga atgccgtcgt taacccaggt taagtggcgg
     2221 cagaaatttc atggaattga acaacgtatt tgctgatgcc agcgccaagc aacgcaaggt
     2281 accgaagggt cagcatgttt ttcgtcaggg tgaaaagacg gaccggatct gtctgatcca
     2341 gcagggactg atcaaggcgt actacgaaac gcgcgacggc aaggaattca tcaagtcgtt
     2401 cattgccgag ggcggcatca ttgccagcct gcaggcgatt gtcgagcgtc agccgagcac
     2461 gttcagcgtg ctggcactgg aagactgcat catactcgag ctgccgaaat cggtgctgga
     2521 tgcccggcag gcgcaggatc cc
//
LOCUS       DASUEG010000033         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1743804, whole genome shotgun sequence.
ACCESSION   DASUEG010000033 DASUEG010000000
VERSION     DASUEG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743804"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..2489
                     /locus_tag="VFX11_00530"
     CDS             144..2489
                     /locus_tag="VFX11_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HEX4937129.1"
                     /translation="MRQPVILLLILLATGCASLVINDYDQRFGAANPARFDQPLATGS
                     TDYWRDVKPVLEQRCVVCHACYDAPCQLKLGSFEGIARGAHTDKVYDGERLLAARTTR
                     LFEDAQSTAGWRELGFFPVLNERRNDPEANLAGSTLAQMLQLKQDHPLPDQPILPDSF
                     DLALDRNQQCVKVEQFEDFRRDYPLWGMPYGLPALPAKERKLLLQWVQEGAPYRAPAP
                     IAPVLERHIQAWERFLNQDSPKAQLMSRYLYEHLYLGHLYFEDGAPPRYFELVRSATA
                     PGQPVQRIATRRPYDDPGVARVYYRILPVREAIVAKTHMPYRLSPTRLQRWQALFLTP
                     AYDVAELPPYDVETASNPFITFRQLPAGARYRFLLEEAQYTVMNFIKGPVCRGQMALN
                     VINDHSWVLFVNPDLPSLDVYADAMAEQLARTSLPGEADSNATPFRWMLYADEEKKFL
                     KAKSDFLNQHVKDRIPLDLNLLWHGDGNNQNAALTIFRHSDSASVVKGLQGDQPQTAW
                     VLTYPLLERIHYLLVAGYDVYGNVGHQLNSRIYMDFLRMEGEFNFLALLPQAQREKVR
                     AQWYRGSVNEVKEYVYQHGNRFDGETGIQYQTDQPLPELYQRIRQTLDPVLDTQYALE
                     NGITDPATLQSLRQIQQIRGTSAAILPQTLVIAIASDRQKKTHYYTLLHHNAYSNISN
                     IFGEEDRRLPDEDQMWLANGLIGAYPSAFLQLDQDEVPELVQHLRTLGNENDYRALLD
                     KFGVRRTDPRFWRFSDNLHQAYRKQAPIEAGWLDYNRLENR"
     gene            complement(2570..3004)
                     /gene="cynS"
                     /locus_tag="VFX11_00535"
     CDS             complement(2570..3004)
                     /gene="cynS"
                     /locus_tag="VFX11_00535"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX4937130.1"
                     /translation="MSEFAATILAAKESRGMTWDALAEAIGMSPVWTCSACMGMNSMP
                     QDKAVALCDVLGLGADVAAALQKYPTKTWDKSVPTDPLIYRLYEVVGVYGDTLKAVIQ
                     EKFGDGIMSAIDFTMHVDKEENPKGDRVVLTLNGKFLPYKAW"
     gene            3003..>3362
                     /locus_tag="VFX11_00540"
     CDS             3003..>3362
                     /locus_tag="VFX11_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937131.1"
                     /translation="MKFLLSVGGHAGGFRTDLDQAGRVMHRIPASILPLSARAAPLSA
                     RINCSLQQEHASCQLRHRRIESCRTGLLRSPHQHELLKNAAMPLRLIFIAALLLTCTF
                     SRTLLAAPLPAPADIEAS"
BASE COUNT          709 a         1175 c          873 g          605 t
ORIGIN      
        1 ctctcacgac gtcaacagcg tcctgcagca ggaagaattc gcgcccatcc ggctgagcct
       61 ggcagaggac gcgaccgcca tcatcgccct gctgtggcaa aaaaccctgg aaatctgacc
      121 caaccgcaac cagttgagac atcatgcgtc aacccgtcat cctgctcctg atcctgctgg
      181 ccaccggctg cgccagcctc gtcatcaacg actacgacca gcgcttcggc gccgccaacc
      241 cggcccgttt cgaccagcca cttgccaccg gtagcaccga ttactggcgt gacgtcaaac
      301 cggtgctgga acagcgctgc gtggtgtgtc atgcgtgtta cgacgcgccc tgccagctca
      361 aactgggctc ttttgaaggc atcgcccgcg gcgcccacac cgacaaggtg tacgacggcg
      421 aacgcctgct ggcagcgcgc accacgcgcc tgttcgagga cgcccaaagc accgccgggt
      481 ggcgcgaact gggcttcttc ccggtcctga acgaacgccg caacgatccc gaggccaacc
      541 tcgccggcag cacccttgcc cagatgctgc agctgaaaca ggaccacccg ctaccggatc
      601 aacccatcct gccggacagc ttcgatctcg ccctggaccg caaccagcaa tgcgtgaaag
      661 tggagcagtt cgaggatttc cgccgcgact atccattgtg gggcatgccc tacggcctgc
      721 cggccctgcc cgccaaagaa cgcaaactgc tgctgcaatg ggtgcaggaa ggcgccccct
      781 atcgagcacc ggcgcccatc gcccccgtgc tggagcgaca tatccaggcc tgggaacgct
      841 tcctcaatca ggattccccc aaagcgcaac tgatgagtcg ctacctttac gaacatctct
      901 atctggggca cctgtatttc gaggacggcg caccgccgcg ctatttcgaa ctggtgcgat
      961 ctgccacagc accgggccag cctgtgcagc gcatcgccac ccgcaggccc tacgacgatc
     1021 ctggcgtggc tcgcgtctac taccgcatcc tgccggtccg cgaagccatc gtcgccaaga
     1081 cccacatgcc ctaccggctc agccccaccc gtctgcaacg ctggcaggcg ctgttcctga
     1141 cgccggccta cgatgtggcg gaattgccgc cctatgatgt ggaaacggcc tccaatccgt
     1201 tcatcacctt ccgccagttg ccggccggag cccgctaccg cttcctgctg gaagaagccc
     1261 agtacaccgt gatgaacttc atcaagggcc ccgtctgccg gggccagatg gcactcaacg
     1321 tgatcaacga tcattcctgg gtactgttcg tcaatcccga cctgccctcc cttgacgtgt
     1381 acgccgacgc catggccgag caactggctc gcacctcact gccaggcgag gccgacagca
     1441 acgccacccc ctttcgctgg atgctgtacg ctgacgaaga gaagaaattc ctgaaagcga
     1501 aatccgattt tctcaaccag catgtcaagg accggattcc gctggatctc aacctgctct
     1561 ggcacggcga cggcaacaac cagaacgccg ccctcaccat tttccgccac tccgacagtg
     1621 ccagcgtggt gaaaggactg cagggcgacc agccccagac cgcctgggtg ctcacctacc
     1681 cgctactgga acgcatccat tacttgctgg tggccggcta cgacgtctat ggcaatgtcg
     1741 gacaccagct gaacagccgc atctacatgg attttctgcg catggaagga gaattcaact
     1801 tcctcgccct gctgccgcag gcgcagcgcg aaaaggtccg cgcccagtgg tatcgcggct
     1861 cggtgaatga agtgaaggaa tacgtgtacc agcacggcaa caggttcgac ggcgaaaccg
     1921 gcattcagta ccagaccgac caaccgctgc cagaactcta tcaacgcatc aggcagacgc
     1981 tggacccggt cctggacacg cagtacgccc tggaaaacgg catcactgat cccgccaccc
     2041 tgcagtcgct gcgccagatc cagcagatcc ggggcaccag tgccgccata ttgccgcaaa
     2101 ccctggtcat cgccattgcc agcgaccggc agaaaaagac ccactactac acgctgctgc
     2161 atcacaatgc ctacagcaac atttcgaaca ttttcggtga agaagatcgt cgcctgccgg
     2221 acgaagacca gatgtggctg gcaaacggac ttatcggcgc ctatcccagc gccttcctgc
     2281 aactggacca ggacgaagtg ccggaactgg tgcaacacct gcggacactg ggcaacgaaa
     2341 acgactatcg cgccttgctg gataaattcg gcgtacgccg gaccgatccc cgcttctggc
     2401 gtttcagcga caatttgcat caggcgtacc ggaaacaggc accgatcgag gccggctggc
     2461 tcgattacaa ccggctggaa aaccgctagc ttccaatcga cctgaaacgc ggctgctcat
     2521 tccccaccaa cagcgggcca caccggcccg ctactgttca caagcaaact caccacgcct
     2581 tgtagggcag aaacttcccg ttcagcgtca gcaccacccg atcgcctttc ggattttcct
     2641 ccttgtccac gtgcatggtg aaatcaatgg cgctcatgat gccatcgccg aatttttcct
     2701 ggatcaccgc cttcagcgtg tcaccataaa caccgaccac ttcatacagc cggtagatca
     2761 gcggatcggt gggcaccgat ttgtcccagg ttttcgtcgg gtacttctgc agcgctgctg
     2821 caacatcagc gcccaggccc agcacgtcac acagggccac cgccttgtcc tgcggcatgc
     2881 tgttcatacc catgcaggca gaacaggtcc acaccggcga catgccgata gcctccgcca
     2941 gcgcatccca ggtcataccc ctggattcct tggcggcaag aatggtggct gcaaattcac
     3001 tcatgaagtt tctcctgtcg gtgggaggac atgctggcgg cttccgcaca gacttggatc
     3061 aggccggcag ggtcatgcac cggattccgg cgtcgatcct gcctctgtca gccagggctg
     3121 ccccgctatc agcacgcatc aactgttccc tccagcaaga gcatgcatcg tgccagctgc
     3181 ggcacaggcg gattgaatcc tgccgcaccg gtttgctacg ctcaccgcac caacatgaac
     3241 tactgaaaaa tgccgccatg ccacttcgtc tgatctttat cgccgccctg ctgctcacct
     3301 gcaccttcag ccgtacctta ttagctgccc ctcttcccgc gccggccgac attgaagcca
     3361 gc
//
LOCUS       DASUEG010000034         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_682861, whole genome shotgun sequence.
ACCESSION   DASUEG010000034 DASUEG010000000
VERSION     DASUEG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682861"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1601)
                     /locus_tag="VFX11_00545"
     CDS             complement(81..1601)
                     /locus_tag="VFX11_00545"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4937132.1"
                     /translation="MIYAHPGQPKALVTFKSRYENFIGGEWVPPVKQQYFKNISPVNG
                     EVFCEIPRSSAEDINLALDAAHKAAETWGKTSATERSNLLLKIADRIEQNLEKIAIAE
                     TWDNGKAVRETLAADIPLTVDHFRYFAGCIRAQEGTMAEIDANTVAYHIHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALAAGNCVVLKPAEQTPASILVLMELLADILPPGVVNIVNG
                     FGEEAGQALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIYFADVM
                     QHEDAFISKCVEGLVLAFFNQGEVCTCPSRALIHESIFDDFMARVIARTKTIKRGDPL
                     DTDTQVGAQASLEQFDKIMSYIEIGRQEGAQVLIGGEIEKMAGSLQKGFYIQPTLLKG
                     TNSMRVFQEEIFGPVVGVTTFRTEAEALAIANDTEFGLGAGVWTRDMNLAYRMGRNIK
                     AGRVWTNCYHAYPAHAAFGGYKKSGIGRETHKKALEHYQQTKNLLVSYDVNPLGFF"
     gene            1958..2794
                     /locus_tag="VFX11_00550"
     CDS             1958..2794
                     /locus_tag="VFX11_00550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4937133.1"
                     /translation="MKPLKGLPRDRTLEQVQHHYRTEKAIADRLRQASREERTHIMAT
                     MYAELFQQVQDHPRLRKRQRVQAVATFNKGMRPLLQRFLQPSDRVAEFGPGDCRFTCE
                     IARDVVFAYGIDISDQRNPADSFPPNFRLIVYDGYRLDAIAEGSLDLVYSDQIIEHFH
                     PDDVAGHFRLVWRLLKPGGRYVFRTPHVLTGPHDVSQYFALEPEGFHLKEWSYVELRA
                     LLEQQGFRDFLPFWNARGVHWPMPYRYFALCEGIIGRLPRRRLRPLIRYWIPSLTMVA
                     VK"
     gene            3056..4339
                     /locus_tag="VFX11_00555"
     CDS             3056..4339
                     /locus_tag="VFX11_00555"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX4937134.1"
                     /translation="MNAHRYFAYTLARICLQSPWEREPMAEAVELSLQGQGPWVNSLI
                     RQVLHRFPRRPGLPALFDFLRQSEGLAAIRLRYQPRICRYPLTWPHVVLPPSLPPLDS
                     VGALCEWLRLTPADLDWFADRNNRAATHTTQKVRHYHVTTIPKRQGGMRLLEVPKSRL
                     KQIQRRIHDDILLQLPLHAAAQGFVRGRTPVAHAGLHVGQAVVLRFDLRDCFLHVHGG
                     RVFQAFRALGFPDDVSRLLMGLCTHRLPQWLSAQLPLDETQRQKARQNHLPQGAPSSP
                     LLSHYALRGVDVRLSAYASALGATYSRYADDLVLSGGEHLRHRFRAIEARAGAIVAEE
                     GFALNFRKSRCVPAAQRQQVTGVVVNRQLNIPRVQFDALKAELHNCVQQGWVSQRPGD
                     VVDYRAHLLGRIGWVMQVNPQRGAKLRGLFERIDW"
     gene            complement(4403..>4947)
                     /locus_tag="VFX11_00560"
     CDS             complement(4403..>4947)
                     /locus_tag="VFX11_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4937135.1"
                     /translation="LVCITLLRCGLKPQALRCLNSRALQSRQPLPAGWDHIQRRTDHH
                     CRIRFASLDLQRPGKRSQTAPPSRFKNLLPTIFARSPLLVQVEHALSNSEALLDAEQL
                     CHRLNISRATLHRALKTSGTHFQQLLEMEKLQRATYWLSVRHATIEETADHLGYSDAS
                     NFRRAFKKWAGCCPSDLRSA"
BASE COUNT          928 a         1408 c         1481 g         1130 t
ORIGIN      
        1 gggcttcccg tcaggttatg cagttgccaa tccagtgcgt tctgcatcaa gagtcgttct
       61 gcttcaaaac gccttccgtt tcagaagaag cccagcggat tcacgtcata actcaccagc
      121 agatttttgg tttgctggta atgctccagt gctttcttgt gggtttcgcg gccgatgccg
      181 gattttttgt agccgccgaa agcagcgtgg gccggatagg catgatagca gttggtccac
      241 acgcgaccgg ccttgatgtt gcgtcccatg cgataggcca gattcatgtc gcgggtccac
      301 acgccggcgc ccaggccgaa ttcagtgtcg ttggcaatgg ccagcgcttc ggcttcggtc
      361 ctgaacgtgg tgacacccac caccggtccg aagatttcct cctggaatac ccgcatggag
      421 ttggtccctt tcagcagggt tggctggata taaaaaccct tctgcagact gcccgccatt
      481 ttttcgatct cgccgccaat caacacctgc gcaccttcct ggcggccgat ttcgatataa
      541 ctcatgatct tgtcgaactg ctccagcgat gcctgcgcgc ctacctgggt gtcggtgtcc
      601 agcggatcgc cacgcttgat ggttttggtg cgtgcaatga cccgcgccat gaaatcatca
      661 aaaatggatt catgaatcag cgcccgcgat ggacaggtgc acacctcgcc ctgattgaaa
      721 aatgccagca ccagtccctc aacgcatttg ctgatgaatg catcttcatg ctgcatcaca
      781 tcggcgaaat aaatgttggg tgacttgccg cccagttcca ccgtcgaggg gatgatattt
      841 tccgccgcgc acttgagaat gtgcgaaccc accggggtcg agccggtgaa ggcaatcttg
      901 gcgatgcgct tgctggtggc cagggcctga cccgcttcct cgccaaagcc gttgacgata
      961 ttcaccacgc cgggcggaag gatatccgcc agcaattcca tcagtaccag aatcgaggcg
     1021 ggcgtctgct cggccggttt caataccaca caattgccgg ccgccagtgc cggtgccagt
     1081 ttccacgctg ccatcagaat cggaaaattc cacggaatga tctggcccac cacgcccagc
     1141 ggttcatgaa tgtgataggc cacggtattg gcatcgattt ctgccatggt gccttcctgc
     1201 gcgcggatgc agccggcaaa atagcggaaa tgatcaacgg tgaggggaat atccgccgcc
     1261 agtgtttcgc gcacggcctt gccgttgtcc caggtttcgg caatggcgat tttttccaga
     1321 ttctgttcga tgcggtcggc gattttcagc agcaggttgg aacgttctgt cgccgacgtc
     1381 ttgccccagg tttcggctgc cttgtgtgcg gcatccagcg ccagattgat gtcttcggcg
     1441 ctggagcggg gaatttcgca aaaaacctca ccgttcaccg gtgaaatatt tttgaaatat
     1501 tgttgtttta cgggcggaac ccattcgccg ccaatgaaat tctcgtaacg ggatttgaag
     1561 gtcaccagtg ccttgggctg gccgggatga gcgtagatca taagcggatt cctttcagtt
     1621 ttaattgcca atggttatta agcggcgcat ttcacgcaca tctggagcaa ctgaaagaca
     1681 gctcaccgca gaaacaggtt gtcagccacg ccacagtgca actaaatcca ccggatttca
     1741 agatcagggg cgtccccatg cggacatctg cgtccggtgt tgtggcaaac gcaattacac
     1801 ccattcaact ttcctgcaac gattagctga tacgatggca acataattgc ttatgttgga
     1861 aggaggcctg ccggcgttgc aggcatgcag cagcacgtca acgatgttat gggtccaggt
     1921 gcctgtctgg gctgtgttga agccagggag aataagattg aaaccgctca agggattgcc
     1981 gcgtgatcga acgctggaac aggttcagca ccattatcgc accgaaaaag ccatcgccga
     2041 ccgcttgcga caggccagcc gggaggaacg tacccacatc atggccacca tgtatgcgga
     2101 gctgtttcag caggtgcagg atcatccgcg tctgcgcaag cgtcagcgtg ttcaggccgt
     2161 tgccaccttc aacaagggca tgcgcccgct gctgcaacgg tttctgcagc cctccgaccg
     2221 ggtggcagag ttcggaccgg gggattgccg ttttacctgc gagatcgccc gcgacgtggt
     2281 gtttgcgtac ggcatcgata tctccgacca gcgtaatccg gctgattctt ttccgcccaa
     2341 tttccggctg atcgtgtacg acggttaccg gctggatgct attgcggaag gttcgctgga
     2401 tctggtgtac agcgatcaga tcatcgagca ttttcatcct gatgacgttg ccggacactt
     2461 tcggctggtg tggcgcctgc tcaagccggg tgggcgttat gtgttccgta ctccccatgt
     2521 gctgacaggc ccgcatgatg tgtcgcaata ttttgccctc gagccggagg ggttccattt
     2581 gaaagagtgg tcttatgtgg agcttcgggc gttgctggag cagcaaggat ttcgcgattt
     2641 tctgcctttc tggaatgcgc gcggtgtgca ttggcccatg ccttaccgct attttgcgct
     2701 gtgcgagggc attatcgggc gccttccaag aaggcgtttg cgtccgctga tccgctactg
     2761 gataccatcg ttgacgatgg tggcagtgaa ataacgttct caatcgatag cctgtgcgtt
     2821 ggcgttggcg ctgctgtccc gtacaatcgt ttcccggtgc ctgagcctgt gttgtccccg
     2881 tacagcacgc tttggggtta ccccaaggtt ccatcctgat ccaccgttgc atcgtatgaa
     2941 cttcgctgcg ctgcgcgcag tcacgttcac acgagcgtag tcgtctcgca cccgagggcg
     3001 cctgcagtgg gccgggtcgg catcaccgac tcacccattg ccgggcctgg atgccatgaa
     3061 tgcccaccgt tattttgcct acacgctggc gcgcatctgc ctgcaaagcc cctgggaacg
     3121 ggagccgatg gccgaggcgg tcgagctgtc gctgcaaggg caggggccgt gggtgaattc
     3181 gctgatccgg caggtgctgc accgctttcc ccgtcgaccg ggtttgccgg cactgttcga
     3241 ttttctgcgg caaagcgaag ggctggccgc gatccgcctg cgctatcagc cgcgcatctg
     3301 ccgctatccg ctgacgtggc cgcatgtggt gttgccaccg tcgttgccgc cgctggattc
     3361 agtgggcgca ctgtgcgaat ggttgcgact gaccccggcg gacctggact ggtttgccga
     3421 tcgcaataac cgggctgcta cacacaccac acaaaaagtg cggcactatc atgtcaccac
     3481 tatccctaag cgccagggcg gcatgcgtct gctggaagtg cccaagtccc gcctgaaaca
     3541 gatccagcgt cgcattcacg atgacattct gctgcaattg ccactgcacg ccgctgcgca
     3601 gggttttgtg cgtgggcgca cgccggttgc gcacgcgggc ttgcatgtcg gtcaggcggt
     3661 ggtgctgcga ttcgatctgc gcgactgctt tctgcatgtg cacggtggcc gcgtatttca
     3721 ggcgtttcgt gcgctgggtt ttccggatga tgtcagccgt ctgttgatgg gattgtgcac
     3781 gcatcgattg ccgcagtggc tgtcggcaca attaccgctg gacgagacgc aacggcaaaa
     3841 agcgcgacag aatcatctgc cccagggtgc ccccagttcg ccgctgctga gccactatgc
     3901 actgcgcggt gtggacgtgc ggttgagtgc ctatgccagc gcgctgggtg ccacctacag
     3961 ccgctatgcc gacgatctgg ttctttccgg tggcgagcac ctgcgccacc gtttccgcgc
     4021 gattgaagcg cgagcgggtg cgatagtggc ggaagaagga ttcgcgctga atttccgcaa
     4081 gtcccgttgc gtgccggcgg cgcaacggca gcaggtgacg ggagtggtgg tgaaccggca
     4141 gctgaatatt ccgcgggttc aattcgatgc actgaaagcg gaattgcata actgcgtgca
     4201 gcagggctgg gtgtcacagc ggcccgggga tgttgtggat tatcgcgcgc atctgctggg
     4261 gcggattggc tgggtgatgc aggtgaaccc gcagcgcggt gcgaagctgc ggggattgtt
     4321 cgagcggatt gactggtagc gaccaggttt gcttacgttt cgccaccaaa acgatgtccc
     4381 tcgtcgactg cattgtttgc cgtcacgcac tccgcaaatc cgaaggacag caccccgccc
     4441 acttcttgaa cgccctgcga aaattcgatg catcgctgta acccagatga tccgcagttt
     4501 cctcgatggt ggcgtggcgc actgaaagcc aatacgtcgc ccgctgcaat ttttccatct
     4561 ccagcaattg ctgaaaatgc gtgccgcttg ttttcagcgc gcggtgcagg gtagcgcggc
     4621 tgatgttgag ccgatggcac agttgttctg cgtcaagcag cgcctcgcta ttgctcaggg
     4681 cgtgctccac ctggaccagt agcggactgc gggcgaatat cgtgggcaac aggtttttga
     4741 accgcgaggg cggcgccgtt tgcgagcgct tgcccgggcg ttgcagatcc aggctggcga
     4801 agcggatgcg acagtggtga tcggtgcgcc tttgaatatg atcccagcca gccggtaatg
     4861 gctgacggct ctgcagcgcg cggctgttca ggcaacgcag tgcctgtggt ttcagcccgc
     4921 agcgcagcag tgtaatgcag accagac
//
LOCUS       DASUEG010000035         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1103091, whole genome shotgun sequence.
ACCESSION   DASUEG010000035 DASUEG010000000
VERSION     DASUEG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103091"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..498
                     /locus_tag="VFX11_00565"
     CDS             118..498
                     /locus_tag="VFX11_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011220535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4937136.1"
                     /translation="MFSHVMLGANDIHTSKKFYDSTLKVLGCQPGVIDEKGRCFYFTK
                     TGVFAISIPINGEPASKGNGSTIGFSAKSEKEVNDWHAAGLANGGVTCEDPPGIREGS
                     GMKLYLAYLLDPAGNKICALYRLP"
     gene            563..1594
                     /locus_tag="VFX11_00570"
     CDS             563..1594
                     /locus_tag="VFX11_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4937137.1"
                     /translation="MEHRQLGRSGLMVPALSLGTATFGGSGEFFKLWGSTQAEEARRM
                     VDVCLEHGLNFFDTADVYSNGLSEQVLGEALKGRRNQALISSKGTFGMGSGPNEVGSS
                     RYHITRAVEASLRRLGTDHIDLYFMHGFDALTPVEETLRALDDLISSGKIGYIGASNF
                     SGWHLMKALATSEKYGLGRYVAYQGYYSLIGRDYEWELMPLALDQGVGSMIWSPLGWG
                     RLTGKIRRGQASATGRIAAGGDVGGPPVEDELLFSVVDVLDEIAQQRDKSIAQVALNW
                     VLSRPSVANVVIGARNEEQLKQNLGAVGWQLSSDEIQRLDAASHREPVYPYWHQKGFD
                     SRNPKPTRW"
     gene            1679..2005
                     /locus_tag="VFX11_00575"
     CDS             1679..2005
                     /locus_tag="VFX11_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937138.1"
                     /translation="MMVFLVACSKSDSQALTEKLNISAFPAAAKIEHCGDVARDGGMM
                     WDCIVAINPEDLPVLLAGHTYRQLSTTAPERVYLAQPKGFKQADWIHVFYNEDTRKAA
                     IATHAP"
     gene            2137..2400
                     /locus_tag="VFX11_00580"
     CDS             2137..2400
                     /locus_tag="VFX11_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4937139.1"
                     /translation="MRFTKLEYGQRIEQAVDFQSEDPAFAGTMRVTWTLQPVEDGTLV
                     SVRAEDVPDGILPDDHAAGMRASLENLARFVESGANQGGSHEK"
     gene            2414..2995
                     /locus_tag="VFX11_00585"
     CDS             2414..2995
                     /locus_tag="VFX11_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX4937140.1"
                     /translation="MNPKVDGYFSSAGRWQQETEMLRTIVLDCGLAEELKWGSPCYTF
                     QDANIVLIHTFKEYCAFLFFKGALLKDARGILVQQTKNTQAARQIRFTSVQDIAKMKA
                     VLKAYIREAVEVEKAGLKVEFKETAEFEVPAEWQENLDKSPALEKAFRALTPGRQRAY
                     LLYFSQPKQSATRVSRIAKYVPKILAGKGLKDQ"
BASE COUNT          775 a          843 c         1027 g          714 t
ORIGIN      
        1 gcgctaaccg gctctaaaag agtgtcccgg ggttctcaat tgagcaagca gggtaaacag
       61 cgcaagcggg tccggaataa ccaaactcaa accgtcaata agaaatggga ttttcatatg
      121 ttcagccatg taatgctcgg cgcaaacgat attcacacgt ccaagaaatt ctatgattcg
      181 actttaaagg ttctcggatg ccagcctggc gtgattgatg aaaaaggtcg ctgcttctat
      241 ttcaccaaaa cgggcgtttt cgcgattagc attcccatta atggtgagcc cgcatcgaaa
      301 gggaatggca gtacgattgg cttttctgca aagagcgaga aagaggtgaa tgattggcat
      361 gcggctgggc tcgcgaatgg gggtgttacc tgcgaagatc caccaggcat tcgtgaaggg
      421 tcgggcatga aattgtattt ggcctatctg ctggaccccg ccggcaataa aatttgtgcg
      481 ttgtatcgtt tgccctgatg agtgcagtgc aggcatgtgc agatgcacca tccgagaatc
      541 ataaattaca ggagagattt tcatggaaca tcgacagcta ggccgctcgg gcctcatggt
      601 gcccgcgctc agtctcggca cggctacctt cggcggtagt ggtgaatttt tcaaactgtg
      661 gggaagcact caggccgaag aggcgcgccg gatggtcgat gtctgcctgg agcatgggct
      721 gaatttcttc gatactgccg acgtgtattc caatggcctg tcggaacagg tgctgggtga
      781 ggcgctgaaa gggcggcgca atcaggcgct gatttccagc aagggaacct tcgggatggg
      841 tagcgggccg aacgaggttg gatcgtcccg gtaccacatc acgcgagcgg tggaagccag
      901 cctgcgtcga ctgggcaccg atcacatcga tctgtatttc atgcatggtt tcgacgcgtt
      961 gacgccggtg gaagagacac tgcgggccct cgatgacctg atctccagcg gcaagatcgg
     1021 ttacatcggc gcctcgaatt tttcgggatg gcatctcatg aaggcgctgg cgacgtcgga
     1081 aaaatacggg ctgggtcgct acgttgcgta tcagggttac tattcgctga ttggccgcga
     1141 ttacgagtgg gagttgatgc cactggcgct ggatcaaggt gtcggctcga tgatctggag
     1201 cccgctcggc tggggccgtt tgaccgggaa gattcgtcgg ggccaagcga gcgctaccgg
     1261 ccgcatcgcc gctggcggcg acgttggcgg acctcccgtc gaagacgaac ttctgttcag
     1321 tgtcgttgat gtgctggacg agattgccca gcagcgcgac aagtcgatcg cgcaggttgc
     1381 gctgaactgg gtgctgtcac gcccttctgt tgccaacgtg gtcatcggcg cacgcaacga
     1441 ggaacagctc aagcagaacc tgggcgccgt cggctggcag ttgtcgtccg acgagatcca
     1501 gcggctggat gcggccagtc atcgtgaacc agtctacccg tactggcatc aaaaaggttt
     1561 cgattcaaga aatcccaagc caacccgctg gtagtgggcc ggtcagtgga gcagatacgt
     1621 gaggttagag tgaatgaaaa caacacgatt gaacattcag cgatggaccc tgttgggaat
     1681 gatggttttt ctcgtggcct gctcaaagtc ggatagccag gccctgacgg aaaaactgaa
     1741 tatttctgcg tttcccgctg ctgccaaaat cgagcattgc ggggatgttg ccagggatgg
     1801 cgggatgatg tgggattgta ttgttgcaat caatccggag gatctgcctg tcctgctggc
     1861 gggtcatacc tacaggcagt tgagcacaac cgcgccggag cgcgtgtacc tcgctcagcc
     1921 caagggtttc aagcaggcgg actggataca cgttttttac aacgaagaca cacgaaaggc
     1981 tgcaattgcg acacatgcac cctgacagta gcggcgaacc cgccaatgcc ggtgctgcaa
     2041 gtcgaatagc gatggagcag tggctgcctc cggctgactt accgggaggc gacacccgga
     2101 ctgggcaaaa cgaccggtga tgctgacgaa gtcgacgtgc gtttcacgaa actggagtat
     2161 gggcaacgga tcgaacaggc tgtggatttc caaagcgagg atcccgcgtt cgccggcacc
     2221 atgcgggtga cctggacgtt gcagccggtg gaagacggaa cgctggttag cgtccgcgcg
     2281 gaagatgtcc ccgacggcat tcttcccgat gatcacgcag cgggcatgcg cgcgtccctg
     2341 gagaatctgg cgcgctttgt ggaaagcgga gccaaccaag gaggttcaca tgaaaaataa
     2401 acgcagcgct ggaatgaatc cgaaagttga cggttatttc agtagcgccg gtcgctggca
     2461 gcaagaaacc gaaatgctga gaacaatagt ccttgattgt gggctggctg aggaattgaa
     2521 gtgggggtcg ccctgctaca catttcagga cgccaatatc gtcctgattc ataccttcaa
     2581 ggaatactgc gcgtttctgt ttttcaaggg cgccctgtta aaggatgcca ggggcattct
     2641 ggtccagcag acaaagaata cgcaggcggc gcgtcagatc cggttcacca gcgttcagga
     2701 cattgcgaaa atgaaggcgg tcctgaaagc ctatatccgt gaagcagttg aagtggaaaa
     2761 ggctggtctg aaagtggagt tcaaggaaac cgccgaattt gaagtgcctg cggaatggca
     2821 ggaaaatctg gacaaaagcc ccgccctgga aaaggccttt cgtgcactga cacccgggcg
     2881 ccagcgtgcg tacctgttgt atttttccca gcccaagcaa tcagcaaccc gggtatccag
     2941 gattgcgaaa tatgtgccga aaattcttgc tggcaagggt ctgaaggatc agtgaatttg
     3001 tttggagatc tgtgcgtagg gctgcttctg gttcggacat tatttgagct ggcattgtac
     3061 agagaccgac gcacaggtaa cggaatactg ctgtccagca cggtgccgtg gttgtagtat
     3121 ctgcaggtgc gccgtggtct ggaggcagga tcagtgacag ttcggcgcag cgtccaggaa
     3181 cccgcacctg cgtagtccga taaggagcaa tccatggaaa tgaccagcgc aacagtcgtc
     3241 cgcccccgtt cccgtcaaaa gcacacaccc gtcgcaccga tacaggcgcg gcgtgtggat
     3301 ttcaatttca gttcggccac ggcaaaacgc tatttctacg ccgacgatcc atttctcag
//
LOCUS       DASUEG010000036         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1279542, whole genome shotgun sequence.
ACCESSION   DASUEG010000036 DASUEG010000000
VERSION     DASUEG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279542"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX11_00590"
     CDS             <1..274
                     /locus_tag="VFX11_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX4937141.1"
                     /translation="SECYRFPELGAAAVEKTNLGIKRISAFIRESAARDGVPCKNPTA
                     IAQVFIFMIRGWYMQVLQANQVVSAVEREEWVNQAVNALLFARKDW"
     gene            560..1372
                     /locus_tag="VFX11_00595"
     CDS             560..1372
                     /locus_tag="VFX11_00595"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4937142.1"
                     /translation="MMFEALALKDGIGSEIRTDVESLLTAEVAANVRRLLVERGVVVF
                     KGLHLTDAQQVALARLMGSVRQEGENGIYKITLDKNANDRRDYLKGSFLWHIDGTHDD
                     VPVFASLLTGITLSTTGGQTEFANSYGAYEALPKEMKAKIEGLKVVHSVEVSMRRADI
                     EPTEVNLAFWRKFPDKTHNLVWTHKSGRKSLVIGCHASHIEGMDKDAGQTLLQELLNW
                     TTQPRFVYRHEWTPGDLLIWDNTGVLHRVEPYPLDCGRMMHRTTLLGEEAFA"
     gene            1458..>2983
                     /locus_tag="VFX11_00600"
     CDS             1458..>2983
                     /locus_tag="VFX11_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4937143.1"
                     /translation="MIRNLPAKWDLEADVVSIGSGGGGLCAAITAHDNGASAVVLERS
                     DQVGGVTAYSMGEVWIPGNHLAAALGIKDSPESGYRYIKGLSMNYGHDSAILNQAVHG
                     PVALKYFEDRIGLKMCAIRNLPDYYYPHVEDSLAEGRYLEVLPFPAATLGEWQFKTRV
                     SPHVPYSMTHDEIFDNGGLANMINWDFSVMSERLGRDERCAGPGLAAYFVKGVLDRKI
                     PLLTGVNAEELVVDGARVVGLRATRDGKDFFVKANRGVVIAVSSYERNPDFAKTLGHQ
                     LNPVSMVMPEIDGAHLRLAGQVGARVARVPDVSMLGFHVPGEEQEEGVPLWRGALPFL
                     GLPHTVLVNRTGKRFSNEAFYRSVYFALDIIDGATQTHPNFPCWAIFDSQAREKYPFG
                     SLMPGQEMPEALGTTADSIAALADKIGVDVQGLAATVAAFNGYCETGNDPEFKRGTFP
                     WGALMCGDPTRKPNANLGPLLKPPFYAVQLHRMAGGGICAAGVVADHHCRAIGWDNKP
                     "
BASE COUNT          638 a          797 c          896 g          652 t
ORIGIN      
        1 cagcgaatgc tatcgttttc cggagctggg ggcggccgcc gttgaaaaaa caaatctggg
       61 gatcaaaagg atttccgctt tcattcgaga aagtgccgcg cgcgacggtg tgccctgtaa
      121 aaatccgacg gcaatagccc aggttttcat cttcatgatc cgcggctggt atatgcaggt
      181 tttgcaggcc aaccaggtgg tttcagcagt ggagcgggag gagtgggtta accaggcggt
      241 caacgcattg ctgtttgccc ggaaggattg gtaagaggga atagtccttc agcatattga
      301 acggcacgct ggtcatcctt tcaaaatttc ccggcccctt tttccgtggc cccgcctaac
      361 agtcggttat ccatcatttt tccagctggc cgcatgtatt gcgcattcgc ggcccctttc
      421 cttgtttgtc gaattttttc gccggtctga aatcagttgc aaccggcaat tgccattaat
      481 agtacacaag tgtactatta aatgaaatgt cagtccgggg cgccctttca tgcccctttg
      541 ccgcacggaa aatataaaaa tgatgtttga ggcgttggct ctaaaagatg gaatcggctc
      601 ggaaattcgc acggatgtgg aaagtctgtt gaccgccgaa gtggctgcaa acgttcgtcg
      661 gctgctggtt gagcgtggcg tggtggtatt caagggcctc catctgaccg atgcacagca
      721 ggttgcgctg gcgcggttga tgggctcggt gcggcaggag ggtgaaaacg gcatctacaa
      781 aatcacgctc gataaaaacg cgaatgatcg ccgggattat ctgaagggat ctttcctctg
      841 gcatatcgac ggcacgcacg acgatgtgcc ggtattcgca tcgctgctga ccggaatcac
      901 cttgtccacg accggtggcc agacagaatt tgccaattcc tacggtgcct acgaagcgct
      961 accgaaggag atgaaggcca aaattgaggg gctgaaggtg gtgcacagcg tggaagtgtc
     1021 gatgcgccgc gccgacatcg aaccgactga agtcaatctt gcgttctggc ggaaatttcc
     1081 cgacaagacc cacaacctgg tgtggacgca caagtccggc cgcaaatcgc tggtcatcgg
     1141 ctgtcacgca tcgcatatcg aaggcatgga caaggatgcg ggtcagacac tgctgcagga
     1201 attgctgaac tggacgacac aaccgcgatt tgtctaccgc cacgagtgga cgccgggcga
     1261 tctgctgatc tgggataaca ccggtgtgct gcatcgtgtt gaaccttatc cgctggactg
     1321 cgggcggatg atgcatcgca ccacgttgct gggcgaggaa gccttcgcct gagtgcgggc
     1381 cgccaacata accagaaaca gtgtttaaac agaaacgaac tttccaaccc aacaataacg
     1441 atgaaagagg gagcgacatg attcgaaatc ttccggcgaa atgggatctt gaagccgatg
     1501 tcgtttccat cggttccggt ggtggcggtc tgtgtgcggc catcacggcc cacgacaacg
     1561 gggcgagtgc agtcgtgctc gaacgttctg atcaggtggg tggcgttacc gcctattcca
     1621 tgggtgaggt ctggattccg ggcaaccacc tcgcggcggc actgggcatc aaggattcac
     1681 cggagagcgg gtatcgctac atcaagggtc tttccatgaa ttacggccat gactcggcga
     1741 ttctcaatca ggccgtgcat gggccggtgg cgctgaaata tttcgaagac cgcatcggtc
     1801 tgaagatgtg cgcgatccgc aatctacccg actactacta tccccatgtc gaagacagcc
     1861 tggcggaagg acgctacctg gaagtactgc cgtttccggc cgcaacgttg ggggaatggc
     1921 aattcaagac ccgtgtgtcg ccccatgttc cctacagcat gacccacgac gagattttcg
     1981 ataacggcgg actggcgaac atgatcaact gggatttcag tgtcatgtcc gagcggctgg
     2041 gtagagacga gcgttgcgcg ggcccgggtc tggcggcgta ttttgtcaag ggtgtgctgg
     2101 accgcaagat tccgctgctc acaggcgtga atgcggagga actggtggtt gacggtgcgc
     2161 gtgttgtcgg actgcgcgcc acccgcgacg gcaaggattt ttttgtcaaa gccaatcgtg
     2221 gtgttgtcat cgccgtcagc agctatgagc ggaatcccga ttttgcgaag acgctgggac
     2281 accagctcaa tccggtatcg atggtgatgc cggaaatcga cggtgcccat ctgcgcctgg
     2341 ccgggcaggt cggcgcgcgc gtcgcccgcg ttcccgatgt cagcatgctg ggttttcacg
     2401 tgcccggcga ggagcaggaa gaaggcgtgc cgttgtggcg gggagccctg ccatttttgg
     2461 ggctgccgca cactgttctg gtcaatcgca caggcaagcg cttttccaat gaggcatttt
     2521 atcgctcggt ctattttgcg ctggatatta tcgacggcgc gacccagacc catcccaact
     2581 ttccctgctg ggctattttc gacagccagg cacgggagaa atatccgttc ggctcgctga
     2641 tgcctggaca ggaaatgccg gaagcgttgg gaaccactgc tgattccatc gcagcactgg
     2701 cagacaaaat cggtgtggat gtacaaggtc ttgccgccac cgttgccgcg ttcaacggct
     2761 actgcgaaac cggcaacgat ccggaattca aacgcggcac attcccgtgg ggcgcactga
     2821 tgtgcggtga tcccacccgc aaaccgaatg ccaatctcgg gccgctgctg aaaccacctt
     2881 tttatgccgt gcaactgcat cgcatggcgg gtggcgggat ctgcgccgct ggtgttgtgg
     2941 cggaccatca ctgccgtgcc atcggctggg acaacaaacc cat
//
LOCUS       DASUEG010000037         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1721557, whole genome shotgun sequence.
ACCESSION   DASUEG010000037 DASUEG010000000
VERSION     DASUEG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721557"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFX11_00605"
     CDS             <1..439
                     /locus_tag="VFX11_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937144.1"
                     /translation="SAQPGKRQGGHMRINLRSIAFSWMFALGLLGLLLLLGAPNVART
                     GTPDVIELPGQLAPAGKDQLTSLQSTIWCALESGQTVRLSVEGRQMQFHGAMAIAPEW
                     RGKALPAPQQKRVDHCVQQRLQQLAGTGREGHRAALTFLFTNI"
     gene            complement(461..1582)
                     /locus_tag="VFX11_00610"
     CDS             complement(461..1582)
                     /locus_tag="VFX11_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HEX4937145.1"
                     /translation="MPSWMRKRVNRVLQPLGTRITAEDRASSLTEYDYRVRVTQLHQE
                     TPRAVTITFDMLEGYRLIYRSGQFVKVSVPIGPTLFQRCYSFSSAPDDNRYSITVQTI
                     FQGRLSRHLVESLRVGDEFYISEPAGEFVLPQERPDEQRYVMVAAGSGIVPVFSLLKD
                     LLGKNPAADIQLIYACRTPDQCLFRRELERLEKLHPGLRLHLQFTRKEGNQHDPGRRL
                     DGQRILERIDNPAHALFYLCGPYGLVKKCLEAFHGAGIDESRIHIELFNAPPAEAEEI
                     ALKPRLVTFLPPTLLGRVRHVRQRQVESLLETARSAGIEIPAQCTAGSCKACKLKVKS
                     GSVILDEPNQLTLEEAREGYVLSCLAYPCENLVVQTPNR"
     gene            1748..2134
                     /locus_tag="VFX11_00615"
     CDS             1748..2134
                     /locus_tag="VFX11_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX4937146.1"
                     /translation="MAADPNDPLYQLLLDEKIEEFNKQRAAGKTVDLRGASLRGRDLR
                     LMNVDGLDMRDAYLRGADIRGVDFRNTQLEGASICEAKISGCFFPRELTAEEIRLSQE
                     KGTRMRYRSIRIVKKSEIPKSEAEED"
     gene            complement(2172..2489)
                     /locus_tag="VFX11_00620"
     CDS             complement(2172..2489)
                     /locus_tag="VFX11_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937147.1"
                     /translation="MAGFIVGSLILTWVLCSALNAFVEYAAIKGWLNRGTAFLSMVVG
                     VLLIAGAMVALAVWGFPESAIAREAVNPYQVSGAIRTSCVIDVLFALGYCAFQLRRFW
                     DDA"
     gene            complement(2492..4069)
                     /gene="prfC"
                     /locus_tag="VFX11_00625"
     CDS             complement(2492..4069)
                     /gene="prfC"
                     /locus_tag="VFX11_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008925708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX4937148.1"
                     /translation="MSHLDEVRKRRTFAIISHPDAGKTTITEKLLLYGNQIQVAGTVK
                     GRGSDRHATSDWMEMEKERGISVTTSVMQFPYKDCIVNLLDTPGHEDFSEDTYRTLTA
                     VDSALMIIDGAKGVETRTIKLMEVCRLRDTPILSFINKMDREIRDPIDLLDEVEHVLK
                     IKCAPITWPIGMGKLFKGVYNLYQDTTYLYATGQGHTIQDVRPIKGIDNPELDAAVGD
                     LADDLRSQLELVRGASHEFDLQEFLDGKLTPVFFGTALGNFGVDHMLDGLVQWAPPPL
                     HRDTDKRTVEAADERFTGFVFKIQANMDPKHRDRVAFMRVCSGHYEKGMKMKHVRLGK
                     EVRISDALTFLAGERAHLEEAWPGDIIGLHNHGTIQIGDTFTAGEDINFQGIPHFAPE
                     LFKRVNLRDPLKSKQLQKGIQQLSEEGATQVFMPLRNNDIIVGAVGVLQFDVVAYRLK
                     DEYKVECSYDLVNVSTARWVYCDDWKMLEDFKKKCHDNLALDGGGYLTYLAPTRVNLE
                     LTMERWPDVRFKETREH"
BASE COUNT          824 a         1251 c         1299 g          804 t
ORIGIN      
        1 ctcagcccag ccgggcaaac gccagggagg tcacatgcgc atcaacctac gatcgattgc
       61 attcagctgg atgtttgccc tcggcctgct ggggctgctg ctgttgctgg gtgcccccaa
      121 cgtagcccgc accggtaccc ccgacgtgat tgagctgccc gggcagctgg caccagccgg
      181 caaggaccag ctgacctcgc tgcagtccac catctggtgc gcactggaga gcggccagac
      241 cgtgcggctc tccgtcgaag gccggcagat gcaatttcat ggcgccatgg caattgctcc
      301 ggaatggcgt ggcaaggccc tgcccgcacc tcaacaaaaa cgggtagacc actgcgtaca
      361 gcaacggctg caacagctgg ccggcaccgg cagggaaggc catcgtgctg ccctgacctt
      421 cctcttcacc aatatctgaa tcaacctcga cggccaggca tcaacgattg ggcgtctgca
      481 ccaccagatt ctcgcaggga taggccagac aactgagcac gtaaccttcc cgcgcttctt
      541 ccagcgtcaa ctggttgggc tcatccagaa tcacactgcc actcttcacc ttcagtttgc
      601 aggccttgca actgcccgcc gtgcactggg cgggaatctc aatccccgcc gaccgcgccg
      661 tttccagcag gctttccacc tgccgctggc gcacgtggcg cacccggccc agcagggtgg
      721 gcggcagaaa ggtcaccaga cggggtttta gcgcaatttc ttctgcctcc gccggcggtg
      781 cgttgaacag ttcgatatgg atccgtgatt cgtcgattcc tgccccgtgg aaagcttcca
      841 ggcatttctt caccagacca taggggccgc acaggtagaa caatgcatgg gcgggattgt
      901 cgatacgctc aagaatgcgc tgcccgtcca gccgccggcc gggatcgtgc tgattgcctt
      961 ccttgcgggt gaactgaagg tgcaggcgca aaccgggatg cagtttttcc agccgctcca
     1021 gttcccggcg gaacaggcac tggtctggcg tgcggcaggc gtagatcagc tggatgtcgg
     1081 cggcgggatt cttgcccagc agatccttga gcagggaaaa caccggcacg atgccggaac
     1141 ccgccgccac catcacgtag cgttgctcat ccggccgttc ctggggcagc acaaattcgc
     1201 cggccggttc cgaaatatag aattcatccc ccacccgcag cgattccacc agatggcgcg
     1261 acaggcggcc ctgaaaaatc gtctgcaccg taatcgagta gcgattgtca tcaggggcgg
     1321 aggaaaacga atagcagcgc tggaacaggg tgggaccaat gggcacggat accttcacga
     1381 actggccgga cctgtagatc aggcgatagc cttccagcat gtcgaaggta atggtgacag
     1441 cgcggggcgt ttcctggtgc agctgggtga cccgcacgcg ataatcgtat tcagtcagcg
     1501 aagacgcgcg gtcctctgcg gtgatccggg ttcccagcgg ttgcagcacg cggttgacac
     1561 gcttgcgcat ccaggagggc atggttcggg acagtgtcct ggcgatcctg ctcacacaga
     1621 ctccggggcg ttatggggat gaactgcacc caacgataac gaagattgcc cctgctaggc
     1681 aacgcattca acggcaagta cgcggaaaat cagctataaa taaagccaga cccccaggag
     1741 atctatcatg gctgccgatc ccaacgaccc cctttaccaa ctgctgctgg acgagaagat
     1801 cgaggaattc aacaagcagc gggctgccgg gaaaacagtg gacctgcgcg gtgcgtcgct
     1861 gcggggccgc gatctgcgtc tgatgaacgt ggacggactg gacatgcgcg acgcctatct
     1921 gcgcggcgcc gacatccggg gcgtggattt ccgcaacacc cagctggaag gtgccagcat
     1981 ctgcgaggcg aaaatttccg gctgtttttt tccccgtgaa ctgactgcgg aggaaatccg
     2041 cctgtcgcag gaaaagggca cccgcatgcg ctaccggtcg atccggatcg tgaagaaaag
     2101 tgaaataccg aagtccgagg cggaagaaga ctgagccgcc ggatcaggcc cgacggccag
     2161 gcaaaacaga ctcaggcatc atcccagaaa cggcgcaact gaaacgcgca gtagcccaac
     2221 gcaaacagca catcgatgac gcaactggtg cggatggcac cactcacctg atagggattc
     2281 accgcctcgc gcgcgatggc ggattccgga aagccccaca ccgccagcgc caccatggcc
     2341 ccggcaatca gcaacacacc gaccaccatg ctcaggaacg cggtgccgcg attcagccag
     2401 cccttgattg ccgcgtattc cacgaaggcg ttgagggcgc tgcacagcac ccaggtgaga
     2461 atcagtgagc caacgatgaa tcctgccatg atcagtgctc gcgggtttcc ttgaagcgga
     2521 cgtcgggcca gcgttccatg gtgagttcca ggttcacgcg agtgggggcc agataggtga
     2581 gataaccgcc accatccagc gccaggttgt cgtggcattt tttcttgaag tcttcgagca
     2641 ttttccagtc gtcgcagtac acccagcgcg cggtggacac gttcaccaga tcgtaggaac
     2701 actccacctt gtactcgtcc ttcaggcgat aggccaccac gtcgaactgc agcacgccga
     2761 cggcaccgac gatgatgtca ttgttgcgca gcggcatgaa cacctgggtg gcaccctctt
     2821 ccgacagctg ctggatgcct ttctgcaact gcttgctctt gagcggatcg cgcaggttca
     2881 ctcgcttgaa cagttccggc gcgaaatggg gaatgccctg gaaattgatg tcttcccccg
     2941 ccgtgaaggt gtcgccgatc tggatggtgc cgtggttgtg caggccgatg atgtcgccgg
     3001 gccaggcttc ttccagatgg gcgcgctcgc cggcgaggaa cgtcagcgcg tcggaaatcc
     3061 gcacttcctt gccgaggcgc acgtgtttca tcttcatgcc tttctcgtag tggccggagc
     3121 acacccgcat gaaggccacg cggtcgcggt gtttcgggtc catgttcgcc tggatcttga
     3181 acacgaagcc ggtgaagcgt tcgtcggcgg cttccacggt gcgcttgtcg gtatcgcggt
     3241 gcagcggggg cggtgcccac tgcaccagac cgtcgagcat gtggtcaacg ccgaagttgc
     3301 ccagcgcggt accgaaaaac accggggtga gtttgccgtc gagaaattcc tgcaggtcga
     3361 attcgtggct ggcgccgcgc accagttcca gctgcgagcg cagatcgtcc gccagatcgc
     3421 ccacggcggc atccagttcc ggattgtcga tgcccttgat ggggcgcacg tcctggatcg
     3481 tgtggccctg gccggtggcg tacaggtagg tggtgtcctg gtacaggttg tacacgccct
     3541 tgaacagctt gcccatgccg atgggccagg tgatgggggc gcatttgatt ttgaggacgt
     3601 gctcgacttc gtccagcagg tcgatgggat cgcggatttc ccggtccatc ttgttgatga
     3661 acgacaggat cggcgtgtcg cgcaagcggc agacttccat cagcttgatg gtgcgcgtct
     3721 cgacgccctt ggcgccgtcg atgatcatca gtgcggagtc aacggcggta agggtgcggt
     3781 aggtgtcttc cgagaagtcc tcgtggcccg gcgtgtccag caggttgacg atgcagtcct
     3841 tgtaggggaa ctgcatgacg gacgtggtca ccgagatgcc ccgctccttt tccatttcca
     3901 tccagtcgga ggtggcatgg cggtcggagc cccggccctt gacggtgccg gccacctgga
     3961 tctggtttcc gtagagcagc agcttttcgg tgatggtggt tttaccggcg tccgggtgcg
     4021 agatgatcgc gaaggtgcgg cgttttctga cttcgtccag atggctcatg atgacctgta
     4081 tcggggtggt tcgggggtaa agaggcggga attatacctg aatcaggcag ggtcggggac
     4141 tgagggttat gctggccggt ggagatggaa tcgcccac
//
LOCUS       DASUEG010000038         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1942568, whole genome shotgun sequence.
ACCESSION   DASUEG010000038 DASUEG010000000
VERSION     DASUEG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942568"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..816
                     /locus_tag="VFX11_00630"
     CDS             154..816
                     /locus_tag="VFX11_00630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4937149.1"
                     /translation="MSNTSTLDLQALYPYLLAHSIREDSLLQRLQAETARDPLARMQI
                     APEQGQFMELIARLIGARRMIEIGTFTGYSSLCLARALPDDGLLLCCDIDAHWTGIAR
                     RYWQEAGLSHRIQLRLAPALDTLDELLRESQHNRFDLVFIDADKENYDAYYERSLQLV
                     RPGGLIMFDNMLWSGRVADERVQDIDTVALRALNLKLKGDDRVDISLLPMADGITLAR
                     KR"
     gene            complement(824..1546)
                     /locus_tag="VFX11_00635"
     CDS             complement(824..1546)
                     /locus_tag="VFX11_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937150.1"
                     /translation="MSGHYMSFEATVTLRRDAETFIANIQRGERASQSLLLQQVLDAF
                     VNQCLQVFFLAPADVAGLSPVSRKLLTSAIATIRKTLQLVLGRITRKLGNREMRPLAL
                     FMDEVMFRERNRPDGVAFIAFELEDDLSEQLRQLQRACRAGHDDVTDAMVTAFQSMAE
                     EGITRFFAEPVATLNLGPVLGKIAEMGVDTTRSVTSTLIRRLFVMLTHEQMAAVVEYF
                     CGMITDEAPDVQTPVVDQVEWA"
     gene            2169..>2660
                     /locus_tag="VFX11_00640"
     CDS             2169..>2660
                     /locus_tag="VFX11_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4937151.1"
                     /translation="MPDPQKHLRTCNLCEAMCGIEITHEQGAILSIKGDPHDPLSKGH
                     ICPKTVALQDLHEDPDRLRYPLLKTPQGWERISWDNAFNEAASRIRSIQAKHGRNAVA
                     LYVGNPSVHNVGTMLTLVPFFNALKTDNRYSATSVDQLAPMLVSMKLYGNQLLFPVPD
                     IDRT"
BASE COUNT          610 a          846 c          696 g          508 t
ORIGIN      
        1 tataaccgac aagaaagccg aacgaattca atatagccag cgcttatgat gacacaccct
       61 ttgccctggg ctggaatccc ggtacatttc cattcacacg ctcctccaca ccatccttta
      121 cacccacttc cacctacacc ggagccggcc accatgtcca atacctccac cctggatctg
      181 caagccctct acccctacct gctggcccac tcgatacgcg aagacagcct gctgcagcgg
      241 ctacaggccg aaaccgcgcg cgatcctctg gcccgcatgc agatcgcgcc ggaacagggc
      301 cagttcatgg aactgatcgc gcgtctgatc ggcgcccgcc gcatgatcga gatcggcacc
      361 ttcaccggct acagcagcct ttgcctggcg cgggcgctgc cggatgacgg cctgctgcta
      421 tgctgcgaca tcgacgcaca ctggaccggc atcgcacggc gttactggca ggaagcgggg
      481 ctttcccatc gcatccagct gcgactcgcc cccgccctcg acacactcga cgagctgctg
      541 cgggaaagcc agcacaaccg tttcgatctg gtgttcatcg atgccgacaa ggaaaactac
      601 gatgcctact acgagcgcag cctgcaactg gtgcgaccgg gcggcctgat catgttcgac
      661 aacatgctgt ggagcggacg ggtggcggac gaacgcgtgc aggacatcga taccgtcgcc
      721 ctgcgcgcac tgaatctgaa actgaaagga gatgacaggg tggacatcag cctgttgccg
      781 atggcagacg gcatcacgct ggcacgcaag cgttgaggga cgttcaggcc cactccacct
      841 gatccaccac cggggtctgc acgtccggcg cctcatcggt gatcatgccg cagaaatact
      901 ccaccacagc cgccatctgc tcatgagtga gcatgacaaa caaacggcga atcagcgtgg
      961 aagtgaccga gcgcgtcgtg tcgacaccca tctcggcaat cttgcccagc accggcccca
     1021 gattgagcgt agccacaggc tccgcaaaga aacgcgtgat cccttcctct gccatgctct
     1081 ggaatgccgt caccatcgcg tcggtcacgt cgtcatgtcc tgcccggcaa gcccgttgca
     1141 actgccgcaa ctgctcactc agatcgtcct ccagttcaaa cgcgataaag gccactccat
     1201 ccggacggtt ccgctcgcga aacatcactt cgtccatgaa tagcgccagt ggacgcatct
     1261 cacgattgcc aagcttgcgc gtgatacgac ccagcaccag ttgcagtgtt ttgcgaatgg
     1321 tagcaatggc actggtcagc aacttgcggc tgaccggact gagccccgcc acatccgccg
     1381 gtgccagaaa gaacacctgc agacactgat tgacgaaagc gtccagcacc tgctgcaaca
     1441 acagggactg cgaagcgcgt tcaccgcgct ggatattggc aatgaaggtt tcggcatcac
     1501 gccgaagagt gacggtcgct tcaaacgaca tgtagtgacc ggacatgcat cctcccgcat
     1561 tgaaacatcc ctgggcctgc cggatttcga ggcgcacatg gtctcaaaac ccgcaggttt
     1621 taatcaacca ttttgcgtga agatctgcta gacggtacaa aaagggcgtg gtttcagaca
     1681 gttacaggga aaatacgcgc aatgccagga ggggagctcg cgctacccga tcgcgaaaaa
     1741 gtgtctgctt caccatagct gaggaggata ttggataaca ggacaatttg tgcgatcggg
     1801 tagtcgagca ctcgataata gactgtataa acttacagaa gcaactcttg acaacgaata
     1861 tttcgcgcgc tttttttgac ctcatcagga ggttggtgct tgctcacttt taatggtttg
     1921 tcccgtctcg atttttcccg gaacaggctg ctcaaggcca attactgctg cggacaccca
     1981 tgatgggtga acgccctgta tagtcccgac tacccctttc cgcactgagc aaacaatcga
     2041 caatcccggc aaaataccgc accatataca gcctgtttgc cctgccaccg gcctgctttc
     2101 ctgactggtg tgtcaggggt taagatggct tcctcttcat ggacgcccgc ccaaatcaag
     2161 gaatcatcat gcccgatccg caaaagcacc ttcggacctg taacctgtgc gaagccatgt
     2221 gtggcatcga gatcacccac gaacagggcg cgatcctgtc catcaagggc gatccgcacg
     2281 acccgctcag caagggccac atctgcccca agacggtggc gctgcaggat ctgcatgaag
     2341 accccgaccg tctgcgctac ccgctgttga aaacgccgca aggctgggaa cggatcagct
     2401 gggacaatgc cttcaacgaa gccgcctcac gcatccgcag catccaggcc aagcatggtc
     2461 gcaatgcagt ggcgctctat gtgggaaatc cgtcggtgca caacgtcggc accatgctca
     2521 cgctggtgcc ttttttcaac gccctgaaaa ccgataaccg ctacagcgca acctcggtgg
     2581 accagctggc gccgatgctg gtgtcgatga aactgtacgg caaccagttg ctgttcccgg
     2641 tgccggatat cgaccgcacc
//
LOCUS       DASUEG010000039         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1036505, whole genome shotgun sequence.
ACCESSION   DASUEG010000039 DASUEG010000000
VERSION     DASUEG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036505"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..575
                     /locus_tag="VFX11_00645"
     CDS             408..575
                     /locus_tag="VFX11_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX4937152.1"
                     /translation="MLYYALLLLIIVVVAAALGFGGIAGSATLIAKILFVAALIALVI
                     SLVVGRRPPPL"
     gene            642..845
                     /locus_tag="VFX11_00650"
     CDS             642..845
                     /locus_tag="VFX11_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX4937153.1"
                     /translation="MNEDIIKGKWKQLKGDAQRKWGKLTNDDLDVLEGNREYLIGRIQ
                     ERTGRARDIVEREVKEFERKLSA"
     gene            complement(999..>3516)
                     /locus_tag="VFX11_00655"
     CDS             complement(999..>3516)
                     /locus_tag="VFX11_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX4937154.1"
                     /translation="PLMPRMYMALAQEDRFPITDILRQTPDIPEQCQWAIFLRNHDEL
                     TLEMVTDRERDYLWQKYATDPRARINLGIRRRLAPLLEYDRRRVELLNSLLLSMPGTP
                     VIYYGDEIFMGDNIYLGDRDGVRTPMQWSPDRNGGFSRADPPRLVLPPIMDSLSGFAS
                     VNVEAQSRDPHSLLNWMRRMLEIRKRQKVFGRGSLKLLTPANRRILAYLRSSADIAGA
                     VAPGTSAETVLCVANVARSAQAVELDLSAFAGRVPVEMIGGATFPPIGRLPYLLTLPP
                     YGFYWFLLAENSAMPAWHVPYPEPMPDLLTLILPDGIQGLLSVTILQRLERDILPAYL
                     PMRRWFAAKNEALASARLVDACVLDDKSASIVVTEVAATSASGTARYLLPLGLVDETA
                     LGGPLPEQLALARTRSGKHLGYLTDAFALKEFGNLLIRLLSREACLPARNGEVRFCRA
                     HADLPLLPLDDADVRWIGVEQSNSSLVVGDAVIIKIIRRVVDGQHPEAEMSRELTARG
                     FANTAPMLGEIVRIDQQGVPHVWAIAQGFIHTQGDGWSWMQSLLERLRQTTMLAVDDA
                     VLLDRTLLQREIAEFASIIGRRLGEMHGVLAAPSDNPDFTPEIADKDQCVRWTRSVQG
                     QVDAALAVLAQPHSWAESWQAEAALALVNARAFLHAAVQRLADSGHGALCCRIHGDLH
                     LGQVLVAQQDAYFIDFEGEPARPLQERRAKNSPQRDVAGMLRSLDYVIARAGNGASAL
                     FDALLEECGSAIHHAFLNAYRQAAEKWEISGTESTGSLVVLFLLEKVAYEVVYEATNR
                     PDWLPVPLLGMVRLAERLGLASIPGPAARGNAEALRKRSV"
BASE COUNT          835 a         1043 c          925 g          713 t
ORIGIN      
        1 agtggcacca ccacttgtcg ggcaagaacg gtacggaatg ctcgatcact gacaggatcg
       61 ctggtaacag gaggtggcag ttcggatgta ctcatcggct atcgatgctc ttatccggcc
      121 agctacggcc gaagggatta atgactgcgg catactgccc ggccaaccgc aaacggatga
      181 atcattatcg ctgacaagca gtagcacagc tgaaattgcg ctggccaccc gttgcaacgc
      241 ccaccatttg taactcttgc ggttaccgtt gcaaggcttg ctgaacttct gcgacttccg
      301 ccgggctgac aggctgcaag cccctgattt tggcacacct cacattggca tgaatcctga
      361 ttagacatcc ataacgacga ggaagccaaa aacagaggtg aatgaaaatg ctctattacg
      421 cactgttgct tctgataatt gtggtagtcg ctgcagcact gggattcggc ggcatcgccg
      481 gtagtgccac actgattgcc aaaatactgt ttgtggcagc gttaatcgcg ctggtcatat
      541 cgctagttgt gggacgtcgg ccaccaccac tttaatccaa tcggccgaca atatacttca
      601 tgcaagaacg atctgtatct ttaccaggga gaaacacgac catgaacgaa gatatcatca
      661 aaggtaagtg gaaacaattg aaaggcgacg cccaacgcaa gtggggcaaa ctcaccaacg
      721 acgaccttga tgttttggag ggcaaccgtg aatatctgat cggacgcata caagagcgca
      781 ccggtcgggc acgcgatatc gtcgaacgcg aagtgaagga attcgagcgt aaactcagcg
      841 catagcagga gcggctgact gccgctgaca aaagggcgct cggtgatttt ttaccaagcg
      901 cctttgcttg tggaaaaaca tattcccctg cattctttca gtgtttttat caggacaata
      961 ttgagttaat tttccgaacg attccctgac gaaattttct acacgctacg ctttcgcaat
     1021 gcttcggcgt tgccgcgcgc cgctggcccc ggaatggacg caagcccaag gcgctctgcc
     1081 agcctgacca tccccagcaa aggcactggc agccagtcgg gtcgattggt ggcctcatat
     1141 acaacttcgt atgccacttt ttccagaaga aaaagcacga ccaggcttcc cgttgactca
     1201 gtcccgctga tctcccattt ttcggccgct tgccggtatg cattcagaaa cgcgtgatga
     1261 atggcgctac cacattcctc cagcaatgca tcgaagagtg cagaggcacc attgcctgct
     1321 ctggcaatca cataatccag tgaacgcagc ataccggcca catcccgttg cgggctgttc
     1381 ttcgccctgc gttcctgcag cggacgtgca ggctcccctt caaaatcgat gaagtatgcg
     1441 tcctgctgcg cgaccagtac ctgccccaga tgcaggtcgc cgtgaatgcg acaacacaac
     1501 gcgccatgac cagagtccgc caaccgctgc acggctgcat gcaaaaatgc gcgcgcattc
     1561 accaatgcaa gtgccgcttc agcctgccat gattcagccc agctatgggg ttgcgccagc
     1621 acggcaagcg ctgcatcaac ctgcccctgc actgacctcg tccaacgcac acactgatct
     1681 ttatcggcga tttcaggtgt gaaatccgga ttgtcggaag gcgctgccag tacaccgtgc
     1741 atttcaccca atcgccggcc aatgatactg gcaaattcag cgatctcacg ttgcagcagt
     1801 gtgcgatcga gcaatacagc gtcatcaact gccagcattg tggtttggcg cagtcgctcc
     1861 agcagactct gcatccagct ccagccatcg ccctgcgtgt gtatgaaacc ctgcgcaatc
     1921 gcccagacat ggggaacacc ttgctgatcg atccgaacga tttctcccag catgggcgct
     1981 gtgttggcaa agccacgggc agtaagttca cgactcattt ctgcttctgg atgctggccg
     2041 tccaccactc gccggattat cttgatgatt accgcatcgc ctaccaccag cgagctgttc
     2101 gactgctcta ctccgatcca gcgcacatcc gcatcatcca gcggcagcag cggcaggtcc
     2161 gcatgcgccc gacagaagcg gacttctcca ttccgcgccg gcaaacaggc ctcccgactc
     2221 agtaaccgga tcagcagatt gccaaactcc ttcagtgcaa aagcatccgt caggtaaccc
     2281 agatgcttac cgctccgcgt gcgggcaagc gccagctgct ctggcaatgg tccaccgaga
     2341 gcggtttcat ccaccagtcc caatggcaac aggtaacggg ccgtgccact ggcgcttgtt
     2401 gccgccactt ctgtgacgac aatcgatgcg gacttgtcgt ccagcacaca ggcatccacc
     2461 agccgcgccg acgccagtgc ttcatttttc gcggcaaacc agcgccgcat gggtagatag
     2521 gccggcagaa tatcgcgctc cagccgttgc aaaatggtca ctgacagcaa accctgaatg
     2581 ccgtcaggca ggataagggt caacagatcg ggcatgggct caggatacgg cacgtgccag
     2641 gcgggcatgg cactgttttc agccagcagg aaccagtaga aaccataagg cggcaatgtc
     2701 agcagatacg gcaggcggcc gattggcggg aatgtggctc caccaatcat ttccacgggt
     2761 acgcgcccag caaaagcaga caggtcaagc tcaacggcct gcgccgaccg ggccacattg
     2821 gccacacata aaaccgtctc ggcggatgta ccgggagcaa cggcaccggc gatatcggcg
     2881 ctgctgcgca gatacgccag gatacgacgg ttggcgggtg tcagcagttt gagtgaacca
     2941 cgaccaaaca ctttctgacg cttgcgaatt tccagcatgc gccgcatcca gttcagcagc
     3001 gaatgcggat cccgtgactg cgcctctacg ttgaccgaag caaatccgga gagagaatcc
     3061 atgatgggcg gtaacaccag acggggcgga tccgcacggg aaaatccacc gttgcgatct
     3121 ggcgaccatt gcattggtgt acgcacacca tcgcggtccc ccagatagat attgtcaccc
     3181 atgaagattt catcgccgta gtaaatcacc ggtgtgcccg gcatcgacag cagaagcgaa
     3241 ttcaacagtt cgacgcgccg gcggtcgtat tcaagcagcg gtgccagacg gcgacgaata
     3301 ccaagattga ttcgcgctcg cggatcggtg gcatatttct gccataaata gtcgcgctca
     3361 cggtcagtca ccatctccag tgtcaactca tcgtggttgc gcagaaatat tgcccactga
     3421 cactgctcgg gaatatccgg tgtctgccgc agaatgtcgg taatcggaaa gcgatcttcc
     3481 tgggccagtg ccatatacat gcgtggcatc aacgga
//
LOCUS       DASUEG010000040         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_726970, whole genome shotgun sequence.
ACCESSION   DASUEG010000040 DASUEG010000000
VERSION     DASUEG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726970"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFX11_00660"
     CDS             <1..846
                     /locus_tag="VFX11_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX4937155.1"
                     /translation="QMLQKKLNRNRNWALREITRLVARARDLGFEVCIGCEDASRADI
                     EFVARVTETAQRAGARRLRFADTVGVMEPFSVFSKIRFLRERSDLELEMHAHDDFGLA
                     TANTLAAVVAGATHINTTVNGLGERAGNAPLEECVLALKHLHQIDTGIDTRGIPELSA
                     LVARASGRPVAWQKSVVGAGVFTHEAGIHVDGLLKDRHNYEGLNPDELGRAHVLVLGK
                     HSGAHMVKTSYQTIGIQLQEWETAALLQRIRRFSTEYKRAPAAADLQQFHDELRMARA
                     VAGGV"
     gene            846..1622
                     /gene="cysE"
                     /locus_tag="VFX11_00665"
     CDS             846..1622
                     /gene="cysE"
                     /locus_tag="VFX11_00665"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX4937156.1"
                     /translation="MKSFSDIFAIKLLAEWRDDIRCVFQRDPAARTTLEVLTTYPGVH
                     AIMAYRVSSRLWRRGWRYLARLLSFVARMASNVDIHPGATIGQRFFIDHGACVVIGET
                     AEVGDDVTLYHGVTLGGTSWNKGKRHPTLGDGVLVGAGAKILGPITVGANARVGANSV
                     VVQDVPMGCTVVGIPGKVVRVREAGRLNPYGIDLDHHLIPDPVGKAIACLLERIDLLE
                     IRLADVSNKPEFAPAIRYQECDADNEICARDCPGGAIAAQ"
     gene            1691..2233
                     /locus_tag="VFX11_00670"
     CDS             1691..2233
                     /locus_tag="VFX11_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937157.1"
                     /translation="MDLQDFKSDMLYFDEPLSPTVDNLLRRAAENYGTGNAERLLLEA
                     FQMARDNLSVLVGLYRFYYYQHRYGMAIEVAHHVMAVVAPRIDFPPQWREITMTSLSN
                     GVLSSIGMVRFYLFALKAAGYLNLRVGRFEEGLAMLKKVVELDTSDRLGARLLLEIVG
                     SHNADVIPFPAQPKKMEARP"
     gene            2230..2562
                     /locus_tag="VFX11_00675"
     CDS             2230..2562
                     /locus_tag="VFX11_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HEX4937158.1"
                     /translation="MNQTALFDDLEELSSAEDFLNYFGVEYNASVVHINRLHIMQRFH
                     DYLQKGKGVVPDDSSDEATRKIYRVLLEQAYQDFVTSDAQTEKVFKVFRMNEPQSTFV
                     SLESLLEG"
     gene            2566..3051
                     /locus_tag="VFX11_00680"
     CDS             2566..3051
                     /locus_tag="VFX11_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEX4937159.1"
                     /translation="MRPAYEYGDEVRLIRNVRNDGTYPGMATGALLVRRGSVGCVYDV
                     GTYLQDQLIYRVMFLESGRTIGCRQEELILASAPWIPSLYEYSDKVLARVALSLRGEV
                     VVPAGQPGSIEKVLRDAPGGVQYHVHFGNGRVLQVPETALMLPAVDSPEDAEAVHADE
                     C"
BASE COUNT          623 a          896 c          945 g          632 t
ORIGIN      
        1 cagatgctgc agaaaaagct caatcgcaat cgcaactggg ccctgcgaga aattacacgg
       61 ctggtggcgc gagcccgcga tctgggcttc gaggtctgca tcggctgtga agatgcctcc
      121 cgcgccgata tcgaatttgt cgcccgcgtt accgaaaccg cgcaacgggc cggcgcccgc
      181 cgcctgcgct tcgccgacac ggtgggcgtg atggaacctt tcagtgtttt cagcaagatc
      241 cgcttcctgc gcgaacgcag cgatctcgaa ctggaaatgc acgcccacga cgatttcggc
      301 ctcgccacgg ccaataccct ggccgctgtc gtggcgggcg caacgcatat caacaccacg
      361 gttaatggcc tgggcgagcg cgccggcaat gcgccgctgg aagaatgcgt gctggcactg
      421 aagcatctgc accagatcga tactggcatc gacacgcgcg gcattccgga attgtctgca
      481 ttggtggcac gcgcgtcggg ccgtccggtg gcctggcaga aaagtgtggt gggcgccggt
      541 gtcttcaccc acgaagccgg cattcacgtg gacggtctgc tgaaggatcg ccacaactac
      601 gaaggattga atccggatga actgggccgc gcccatgtgc tggtgctggg caagcattcc
      661 ggtgcccaca tggtgaaaac cagttaccag acaatcggca tccagttaca ggaatgggaa
      721 acggcagccc tgttgcaacg catccgccgc ttttccaccg agtacaagcg ggcgccggct
      781 gcggcggatc tgcaacaatt tcacgacgaa ctgcgcatgg cacgcgcagt agccggaggt
      841 gtctgatgaa atccttcagc gatattttcg ctatcaagct gctggcagaa tggcgtgacg
      901 acatccgctg cgtgttccag cgtgatcccg ctgcccgcac gacccttgaa gtgctcacca
      961 cctatcctgg cgtgcacgcc atcatggcct atcgcgtcag cagccggttg tggcggcgtg
     1021 gctggcgtta tctggcgcgg ctgctttcat tcgtcgcgcg catggccagc aatgtcgaca
     1081 ttcacccggg tgctaccatc ggccagcgct ttttcatcga ccacggcgcc tgcgtggtga
     1141 tcggtgaaac cgccgaagtg ggtgacgatg tcaccctgta tcacggcgtc accctgggtg
     1201 gcaccagctg gaacaagggc aagcgtcacc ccacgctggg cgacggcgta ctggtcggcg
     1261 ccggcgccaa aattctcggc ccgatcacgg tgggtgcgaa tgcgcgggtg ggggccaatt
     1321 ccgttgtggt gcaggacgtg cccatgggct gcaccgtagt gggtattccg ggcaaggtgg
     1381 tgcgcgtgcg ggaagcgggt cgcctcaatc cctacggcat cgatctggat catcacctga
     1441 ttcctgatcc ggtaggcaag gccattgcat gtctgctgga acgcatcgac ctgctggaaa
     1501 tccggctggc ggatgtgagc aacaagccgg agtttgcgcc ggccatacgt taccaggaat
     1561 gcgatgccga taacgaaatc tgcgcgcggg actgtcccgg cggcgccatc gcggcacagt
     1621 gaccatcgcg gctcagttaa aaaaactgct acgcaatcat caccagtatt caccacaggc
     1681 actcaccatc atggacttgc aggacttcaa aagtgacatg ctctacttcg atgaaccgtt
     1741 atcgcccacg gtcgacaacc tgctccggcg cgccgccgaa aactatggaa ccggcaatgc
     1801 cgaacgcctt ctgctggaag catttcagat ggctcgcgac aatctttcgg ttctggtcgg
     1861 actatatcga ttttactatt accagcaccg atacggcatg gccattgaag tcgcgcacca
     1921 tgtcatggca gttgtcgcgc cgcgcatcga tttcccgccg cagtggcgcg aaatcaccat
     1981 gacgtctttg tccaatggcg ttctcagttc catcggaatg gtgcgcttct atctgttcgc
     2041 actcaaggcg gcgggttatc tgaatctgcg tgtcggccgg ttcgaggaag gtctggccat
     2101 gctgaagaaa gtggtggagc tggatacctc cgatcgcctg ggtgcccgcc tgctgctgga
     2161 gatcgtgggc tcgcataacg ccgatgtgat tccgtttccc gcccaaccca aaaagatgga
     2221 ggcacggcca tgaaccagac tgccctgttc gacgatctgg aagaactcag ttccgccgag
     2281 gattttctga attattttgg cgtggagtac aacgcaagcg tggtgcatat caatcgcctg
     2341 cacatcatgc agcggtttca cgactatctg cagaagggca aaggcgtggt gccggacgat
     2401 agcagcgatg aagcgacccg caagatttat cgcgtgctgc tggaacaggc ctatcaggat
     2461 ttcgtcacct ccgacgcgca gactgagaag gtgttcaaag tgtttcgtat gaatgaaccg
     2521 cagtccacgt ttgtgtcgct cgaaagcctg ctggagggct gagccatgcg cccggcctac
     2581 gaatacggtg atgaagtccg gctgatccgc aacgtgcgca atgacggtac ctatccgggc
     2641 atggccacag gcgcattgct ggtgcggcgc ggttcggtgg gctgcgtgta tgacgtgggc
     2701 acttacctgc aggatcagct gatctaccgg gtgatgtttc tggaaagcgg gcgcaccatt
     2761 ggctgccggc aggaggaact tatcctcgcc tccgccccct ggattcccag tctgtatgag
     2821 tacagtgaca aagtgctggc acgtgttgcg ctgtcattgc ggggcgaagt agtggtgccg
     2881 gccgggcagc ctggcagcat cgaaaaagtg ctgcgcgatg caccgggcgg cgtgcagtat
     2941 cacgtacatt ttggcaatgg tcgtgtactg caggtgccgg aaacagcgct gatgctgccg
     3001 gctgttgatt ctcctgagga tgccgaggcc gtgcatgcag atgaatgctg ataccgatat
     3061 cgcctatcac ctgctacagg tggcacagca gaaatt
//
LOCUS       DASUEG010000041         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1036284, whole genome shotgun sequence.
ACCESSION   DASUEG010000041 DASUEG010000000
VERSION     DASUEG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036284"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="glpD"
                     /locus_tag="VFX11_00685"
     CDS             <1..1176
                     /gene="glpD"
                     /locus_tag="VFX11_00685"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX4937160.1"
                     /translation="NDSIHYGFEYADCRVDDARLVVANALAAQSRGARIQVRTRCIDA
                     VRVNGLWHITLQDLVTGSIHHCRARALVNAAGPWAQRFIEQNLRAQSPRRIKLIKGSH
                     FVTPKLYEGEQAYILQNEDQRIVFVIPYNGDFTLVGTTDKAYEGDPAKVEMDGEEEQY
                     LLNVVNQHFKRQIGSDDILWRYSGVRPLCDDESDSPSAMTRDYTLETQADENGKAPLL
                     SVFGGKITTYRRLAEAALLALKPHFPTMGSPWTRNATLPGGDIGEQTFAHWLQALQQQ
                     YAWLPQPLLQRFANAYGTRVNELLQGCSGMDDLGADFGAGLTQREVEFLLREEWARTA
                     DDILWRRSKLGLYFSAEMRAQLESFMQSRLSEAVPATSTVAPAPSTAGANEPLRRAC"
     gene            1459..2478
                     /gene="glsA"
                     /locus_tag="VFX11_00690"
     CDS             1459..2478
                     /gene="glsA"
                     /locus_tag="VFX11_00690"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX4937161.1"
                     /translation="MKNLKSVASLIVAGWLGACALPVAAVELSAANIETALQSAYSKY
                     KVLKEGANADYIPALAKVDSNLFGIALVTVDGKVYSVGDIKSEVSIQSISKVFTMALV
                     MDESGVESIANNMGVDATGQVFNSIVAIEQYKGAEMNALVNPGAIAATSMVKGDNYDG
                     IWNKILDKYSDFAGRKLTVLEDVYQSEAATNQRNRAIAALMSAYGHITTDPVRATDIY
                     TRQCSVGVNAKDLAQMAATLANAGKNPVSGKQVMNADNVPETLAVMATAGLYDDSGKW
                     LYRTGLPAKSGVGGGIIAVAPGKFGIAVISPPLDAAGNSVRAQKAIADISNALGGNPY
                     QVSAR"
     gene            2545..>2899
                     /locus_tag="VFX11_00695"
     CDS             2545..>2899
                     /locus_tag="VFX11_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937162.1"
                     /translation="MRFRRSLTLACGILVLTAGSLRAETLAEPPIPAIKHWLPPTPAA
                     DSEWDWIQLTSGEWLKGTLRSLEQDILDFDSDELGILELDWEDVQQVMSAKPMAILYG
                     DGATAVGYLTTENDQL"
BASE COUNT          621 a          879 c          861 g          538 t
ORIGIN      
        1 aacgacagca ttcattacgg cttcgaatac gccgactgcc gggtggacga cgcccgcctg
       61 gtggtggcca atgccctggc cgcgcaaagc cgtggcgccc gcatccaggt gcgcacccgc
      121 tgcatcgacg ccgtgcgggt gaacggcctg tggcacatca cgctgcagga tctggtcacc
      181 ggcagtatcc accattgccg agcccgcgct ctggtgaatg ccgccggccc ctgggcccag
      241 cgcttcatcg aacagaacct gcgggcgcaa tcgccgcgcc gcatcaagct gatcaagggc
      301 agccacttcg tcacgccgaa actgtatgaa ggcgagcagg cgtacatcct gcagaacgaa
      361 gatcagcgca ttgtgttcgt gattccctac aacggcgatt tcaccctggt gggtaccacc
      421 gacaaggcct atgagggcga tcccgccaaa gtggaaatgg atggcgaaga ggaacagtac
      481 ctgctgaacg tggtgaacca gcacttcaag cgccagatcg gcagcgacga catcctgtgg
      541 cgctattccg gtgtacgtcc gctgtgcgac gacgaatccg actcaccctc ggccatgacc
      601 cgcgactaca ccctggaaac ccaggcggat gaaaacggca aggcgccgct gctgagtgtg
      661 ttcggcggca agatcaccac ctatcgccgg ctggcggaag cagcgctgct ggccctgaaa
      721 ccccattttc ccactatggg cagcccctgg acccgcaacg ccaccctgcc cggcggcgat
      781 atcggcgagc agacgtttgc gcactggctg caggcactgc aacagcaata tgcgtggttg
      841 ccacagccgc tgctgcaacg attcgccaac gcctatggca cccgcgtgaa tgaactgctg
      901 cagggctgtt ctggcatgga cgatctgggc gcggatttcg gcgccggcct gacccaacgc
      961 gaagtggaat tcctgttacg ggaagaatgg gcgcgcacgg cggatgatat cctctggcgg
     1021 cgcagcaaac tcggcctgta tttcagcgcc gaaatgcgcg cacagctgga gagcttcatg
     1081 caatcccgct tgtctgaggc ggttccggca acatcaaccg tcgcaccggc cccgtccacc
     1141 gccggcgcca acgaaccctt gcgccgcgcc tgctgaggca cggcgctttt tcagcaccga
     1201 ttttttagca cccccctttc acaatcccct tttcacacga ccctcactct tgttgtgatc
     1261 gccaggaccc acccccttgg gccctggcat tttttcagcg cagcttttac cgtgactttc
     1321 gccgcgacgt gcccggtact gcccgcagct tgacgtaaaa aatcaattgt cgttagatgg
     1381 ggcagcacat ccatcgcaaa tcgacccaac ccgtttcatt gcctccaatc ctgaaccctc
     1441 aacgtcggga gtcgacccat gaaaaatctc aaaagcgtgg ccagtttgat tgtggcaggc
     1501 tggctcggcg catgcgcgct gccagtggcg gccgtggaac taagcgcagc gaacattgag
     1561 actgcgctgc agtcggcgta cagcaagtac aaggtgctga aggaaggcgc caacgccgat
     1621 tacattccgg cactggccaa ggtggattcg aacctgttcg gcattgcgct ggtgacggtg
     1681 gacggcaagg tgtacagcgt gggcgacatc aagtcagaag tgtccatcca gtccatctcc
     1741 aaggtattca ccatggcact ggtgatggac gaatccggcg tggaatccat cgcgaacaat
     1801 atgggcgtgg atgccaccgg ccaggtattc aattccatcg tcgcaatcga gcagtacaag
     1861 ggcgccgaga tgaatgcact ggtcaatccg ggtgccattg ccgccaccag catggtcaag
     1921 ggcgataatt atgatggcat atggaacaag atcctggata aatacagcga ttttgccgga
     1981 cgcaaactga ccgtgctgga agacgtgtat caatcggaag ccgccaccaa tcagcgcaac
     2041 cgtgccattg ccgcgctgat gtcggcctac ggtcacatca cgacagatcc ggtgcgcgcc
     2101 accgatattt atacccgcca gtgttccgtg ggcgtgaacg cgaaagatct ggcgcagatg
     2161 gccgcgaccc tggccaacgc cggcaagaat ccggtgtccg gcaagcaggt gatgaatgcc
     2221 gacaatgtgc cggaaaccct ggcggtcatg gcaacggcgg gcctgtatga tgattcgggt
     2281 aaatggttgt acaggacggg cctgccggcc aagagcggcg tgggcggtgg catcattgcg
     2341 gtggcgccgg gcaagttcgg catcgcggtg atctcgccgc cgctggacgc agcgggcaac
     2401 agcgtccgcg cgcaaaaggc cattgctgat atttccaatg cgctgggtgg caatccctat
     2461 caggtcagcg cgcgataacc cgcgtccaga aaatggcgag tgtcagtctg acactttttc
     2521 aaatttattt agcagcaggt tttgatgcga tttcgaagaa gtctgaccct ggcgtgtgga
     2581 attctggtac tgaccgccgg cagcttgcgg gcagaaaccc ttgcggaacc tcccataccg
     2641 gccatcaagc actggctgcc gccaaccccg gcggccgatt ccgaatggga ctggattcag
     2701 ctgacttccg gagaatggct gaagggcacg ctgcgcagcc tggaacagga cattctggat
     2761 ttcgacagcg acgagcttgg catactggaa ctggactggg aagatgtgca gcaggtgatg
     2821 tccgcaaagc ccatggcgat tctgtacggt gacggcgcca ccgcggtagg ctatctgacc
     2881 accgaaaacg atcagctgg
//
LOCUS       DASUEG010000042         4920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_550142, whole genome shotgun sequence.
ACCESSION   DASUEG010000042 DASUEG010000000
VERSION     DASUEG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550142"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFX11_00700"
     CDS             complement(<1..1233)
                     /locus_tag="VFX11_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4937163.1"
                     /translation="MSFELNRKLAAKRAPLPTTADIDPVTASIIRGALETVCFEACAH
                     LGRAASSPIINQSNERNSSIIDSNGRLAVGSVGTPHLTFVTQLTVRYGLMHFEDYDWG
                     PGDVFLGNDPDHGGGHLPDYNVYAPVFDDNGELVLVQALQAHQGDTGGKDPGGFTLEA
                     TDIFTEGLAIPCLKLVHRGEKRRDVIKLVERNNRFATFSGDLAAMIGGVEMAAREHQA
                     LIRKYGTDKVRAALNYTVENTEKRFREEIARWPDGTYEADVWIDADTQGTPDIKVHVA
                     CKVAGDQLTVDLTGTDDRQNLVGVWNTFGNSRGYIMIQLCAMIDPAIPKNEGLFNAVE
                     FIIPEGCIAHPPPNKPAALGSFHPACEITEAVCIALSKVVPERAQPQVYKIGMPNAVI
                     GFYDTGMMWMDQGVDSRSM"
     gene            complement(1325..2305)
                     /locus_tag="VFX11_00705"
     CDS             complement(1325..2305)
                     /locus_tag="VFX11_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4937164.1"
                     /translation="MAIAPNIEELKEEAKRYIRYEKDTENKIAYLTFNRPEALNATTT
                     GMRQYYADLVFKANVDDDVKVLVIRGEGKHFGSGGDLQEQADMYSDSGEDMSLLHEFG
                     INDPSVKYPPKGSYRFLHSMTDHYAKAPAGNRPLQDFKKISIIETKGYCYGWHFYQAA
                     DADLLISSDDTLFGHPAFRYAGWGPRLWTWIEQIGLRKFSEMLFTGRPFTAAEMYETN
                     FINSVVPLEKLEAETKKYAMACSRTRPTDVVQVQKTFLELYKQYRGEYMGSLLTGFVE
                     GMLPMMRNDRKDQAGVDSDTFDKGLNNVVKDIDMLYPPEWRMGMSNRKKP"
     gene            2738..3532
                     /locus_tag="VFX11_00710"
     CDS             2738..3532
                     /locus_tag="VFX11_00710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4937165.1"
                     /translation="MVNSSDKPVVLITGASSGIGDAIARRFAKQGWCIVAVARRQQRL
                     EQLRQELADITEVEFLVADVTEKDAPQRAVDLALGAFGRLDCLVNNAGAGKWAPPGET
                     DDATIDEVLDISLKAPFRFARESLRAMKSGSSIINIGSVFGLLGGMDGGIYCTAKAGM
                     IGMSQAFAVQYGAKGIRSNVVAPGVVKTEMTAAAWDFPGFQRTNQELTPFNRDGTVED
                     VANTVFFLASQEGSYINGQTIALDGGWSTTKYVAPEALICERVMPK"
     gene            3821..4141
                     /locus_tag="VFX11_00715"
     CDS             3821..4141
                     /locus_tag="VFX11_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937166.1"
                     /translation="MSTTAAVIDGAWSLTVKAPNGTQTSVVTLKSEGDVLTGTIANDS
                     FGVQSIEDGKFDGQNLSWKSKITKPMKLTVSYHATLDENNNISGHIKAAMAKILFSGT
                     PVAK"
     gene            complement(4458..>4920)
                     /locus_tag="VFX11_00720"
     CDS             complement(4458..>4920)
                     /locus_tag="VFX11_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4937167.1"
                     /translation="LIGWTGKVLADHPDQLAELVKDPSLINNTIDEILRFEPPSPVQA
                     RYVTQDVEHHGQIVPKGSVLLLLNASANRDERKYQNPDKFDIRRKIDQTLTFGHGIHF
                     CLGSHLAKLEGRVALEEVIKRFPRWKIDWSKAKQANTSTVRGWETLPVITY"
BASE COUNT         1049 a         1261 c         1371 g         1239 t
ORIGIN      
        1 catggagcgc gagtccacgc cctggtccat ccacatcatg ccggtgtcgt aaaatccgat
       61 gacggcgttg ggcatgccga tcttgtaaac ctgtggctga gcccgttccg gcaccacttt
      121 cgacagcgct atgcacaccg cttccgtgat ttcacaggcg ggatggaaag atcccagcgc
      181 ggcaggtttg ttcggtggcg ggtgcgcaat gcaaccttcg ggaataatga actcaaccgc
      241 gttgaaaaga ccttcattct tgggaatggc aggatcaatc atggcgcaca gctgaatcat
      301 gatgtagcca cgcgaattgc cgaatgtgtt ccatacgccg accagattct ggcggtcatc
      361 ggtgccggtg agatccaccg taagctggtc gccggccact ttgcaggcga cgtgtacctt
      421 gatgtccgga gtgccctgcg tgtccgcgtc aatccacaca tcggcttcat aggtgccatc
      481 gggccagcgg gcaatttctt cgcggaagcg tttttccgta ttttcaacgg tgtaattcag
      541 cgcggcccgt accttgtcgg taccgtattt tcgaatcagc gcctgatgtt cccgtgcagc
      601 catttcaacg ccgccgatca ttgccgccag gtcgccggag aaggttgcaa agcggttgtt
      661 gcgctccacc agcttgatca cgtcgcgccg tttttccccg cgatggacca gcttcaggca
      721 gggaatcgcg agtccttcgg taaagatatc ggtcgcttcc agcgtgaatc cacccgggtc
      781 cttgccgccg gtatcgccct gatgcgcttg cagcgcctgc accagtacca gttcaccgtt
      841 atcgtcgaaa accggtgcat aaacgttgta atccggcaga tgtccgccgc catgatcagg
      901 gtcattgccg aggaaaacat cgccggggcc ccagtcgtaa tcctcgaagt gcatgagtcc
      961 gtatcgaact gttaattgcg tgacgaaggt gagatggggt gttcccacgg aacccaccgc
     1021 caggcgcccg ttagagtcaa ttatcgatga attacgctca ttggactggt tgataatggg
     1081 ggatgaagcg gcgcggccca aatgggcgca ggcttcgaaa cagaccgttt ccagtgcgcc
     1141 ccggatgatg cttgccgtca ccggatcaat atcggcggtg gtgggcagcg gcgcccgctt
     1201 ggcggccagc tttctgttta actcaaacga cattggggaa ttcctctttc attgaacctg
     1261 tcgagaccag accacttcac tctcaaaatc atttcattgt ccaaaccact tcaccgcgct
     1321 gcattcacgg cttcttccgg tttgacatgc ccatgcgcca ctccggcggg tagagcatgt
     1381 cgatatcctt gaccacattg ttcaggcctt tatcgaaggt atcgctgtcc acacccgcct
     1441 gatccttccg gtcattgcgc atcatcggca gcatgccttc cacgaaaccg gtcagcaggc
     1501 tacccatgta ttcaccacga tactgcttgt agagttcgag aaaggttttt tgcacctgca
     1561 ccacatcggt cgggcgggtg cgcgaacagg ccatggcgta ttttttggtt tctgcttcca
     1621 gtttttccag cggtaccacg ctgttgatga aattggtttc gtacatttcc gccgcggtaa
     1681 acgggcgacc ggtgaacagc atttccgaga acttgcgcag tccgatctgc tcgatccagg
     1741 tccacagacg tgggccccag cctgcgtagc gaaaggcggg atggccaaac agcgtgtcgt
     1801 cggaggaaat cagcaaatcg gcgtcggccg cctggtagaa gtgccagccg tagcagtagc
     1861 ctttggtctc gatgatgctg attttcttga agtcctgcag cggacgattg ccggcgggcg
     1921 ctttcgcata gtgatctgtc atgctgtgca ggaagcggta ggatcccttg ggcgggtact
     1981 tgacgctggg atcgttgatg ccaaattcat gcagcaggga catgtcttcg ccggagtcgg
     2041 aatacatgtc cgcctgttcc tgcaaatcgc cgccgctgcc gaaatgtttg ccctcgccgc
     2101 gaatcaccag caccttgacg tcgtcatcta cgtttgcctt gaaaaccaga tctgcgtagt
     2161 actggcgcat gccggtggtg gtggcgttca gtgcttccgg gcggttgaat gtcaggtacg
     2221 cgatcttgtt ttcggtatct ttctcgtagc gaatgtatcg cttggcttct tctttgagtt
     2281 cttcaatgtt cggtgcgatg gccatggttt accgcctgtc agggaaacgc tggaaacgaa
     2341 atgcaggatg ctctgtgggg ttgtaccacg atggctcctg agcgtcctga cagttcgtgc
     2401 cacggactct accgtcagtg cagaccctta cccgcgacct gattcaacga tttttcgggc
     2461 gtttcccgcc cggcaagaca gaaccagccc gtactttgac gggcagatgg cggcgcaggc
     2521 tccaaatagt ctgaagagga gtaatgaaat gcctgcgtct gcagttcagc agattcctgg
     2581 tacagatcga gtcgtcagaa aagcagaatt atgtttaaga tcgctcgtat ttttatcaat
     2641 caggatgctc agtccgaatg ggtgattgac gcttattttt cctgcataac gtgtttgatt
     2701 taaaaagaat tatgttgagg cggaggcact acatcacatg gtcaacagtt cagataagcc
     2761 ggttgtattg attacgggtg ccagttccgg gatcggtgat gccattgccc gcaggtttgc
     2821 aaaacaaggc tggtgtattg tcgctgtcgc ccggcgtcag caacgccttg aacagctcag
     2881 gcaggaattg gcagacatta cggaagtgga atttctggtg gctgatgtta ccgaaaagga
     2941 tgcgcctcaa cgcgcagtgg atcttgcgct gggtgctttt ggccggctgg attgtctggt
     3001 gaacaacgca ggggcgggca agtgggcgcc gccaggtgaa accgatgatg caacgatcga
     3061 tgaagtactg gatatcagtc tgaaagctcc attccgcttt gcccgggaat cgctgagggc
     3121 catgaaatcg ggctcgtcca tcatcaacat cggttctgtt ttcggcctgt tgggtggaat
     3181 ggatggcggc atttactgca ccgcgaaagc cggcatgatt gggatgagcc aggcgtttgc
     3241 cgtgcaatat ggtgccaaag gtattcgttc caatgtggtt gcccctggtg ttgtcaaaac
     3301 tgaaatgacg gcggccgcct gggattttcc cggattccag cgtaccaatc aggaactgac
     3361 gcctttcaac cgcgacggca ccgttgagga tgtggccaat acagttttct ttctggcctc
     3421 gcaggaaggc agctacatca atggccagac catcgcgctg gatggtggtt ggtctacaac
     3481 aaaatatgtc gcgccggaag cgctcatctg tgagcgtgtg atgccaaaat aaagccaggt
     3541 gcgtatagtt aagtgctatt ttcgtcgcag gtatgttaaa ccggttcaac ataagtctgt
     3601 gaatagcgat gttcagggat aacgcttttt tggttttttt aatcgttact tgcctagttg
     3661 aagtcttcaa tattttgaat ggcgatgaac agaattatgc cgatttttaa gctgattttg
     3721 ccggtttttc gtggtgtctg gcttgtcgca cgtgtatagt tacgtgagca tggttgtatg
     3781 ctcatagaga ttcaaaacaa taaattttct ggaggaaatt atgtctacta ctgcagcggt
     3841 gatcgacggt gcgtggagcc tgactgtgaa agcgcccaac ggcacacaga ccagcgtggt
     3901 gactctgaaa tctgagggtg acgtacttac tgggaccatt gccaacgatt cctttggcgt
     3961 tcagtctatt gaagacggca agtttgacgg ccagaacctg agctggaaaa gcaaaatcac
     4021 caagccaatg aagttgaccg ttagctatca cgctactctg gatgaaaaca acaacatcag
     4081 tggtcatatc aaagccgcaa tggcaaagat tctgttttcc ggaactccgg ttgccaaata
     4141 aaaatttgtc cattgaatga tgtggaatgg cgatgagcca acgttttgtg cgttggctta
     4201 cccaatttgt ttgacgcagt accgcaccaa ttgatgtggt accccatccc tgatgcctgg
     4261 cacggtctct gtcgcccggc gcttcaattt ttcaaagacg cttaggtcgc acctgcaggc
     4321 attgcgaatg cggatcgccg gaatcgctgg cgtcaggttt gcgggccggt gtgcggcaat
     4381 tgctggaatc acaggcatta aaaaaggtgg ctgcctttgc tgcagccacc tttgttgttc
     4441 tgctatttca atgccaatca gtaggtaatg acaggcaatg tttcccagcc gcggaccgtg
     4501 gaagtgttgg cttgctttgc cttgctccaa tcgattttcc agcgtgggaa tcgcttgatg
     4561 acttcttcca gtgcaacccg tccctccagt ttggcgagat ggctgcccag gcagaaatga
     4621 atgccgtggc cgaaagtcag tgtctgatca atcttgcggc gaatatcaaa cttatccgga
     4681 ttttgatatt tccgttcatc ccggttggcg gatgcgttga gtagcaacag gacgctgcct
     4741 ttcggaacaa tttgaccatg gtgctcgaca tcctgtgtca cgtaacgggc ctgtaccggt
     4801 gaggggggct cgaaacgcag aatctcgtcg atggtgttgt tgatcaggct gggatcttta
     4861 accagctccg ccagctggtc cgggtgatct gccagtacct tgccagtcca gccaatcagt
//
LOCUS       DASUEG010000043         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1721215, whole genome shotgun sequence.
ACCESSION   DASUEG010000043 DASUEG010000000
VERSION     DASUEG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721215"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1607)
                     /locus_tag="VFX11_00725"
     CDS             complement(120..1607)
                     /locus_tag="VFX11_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4937168.1"
                     /translation="MTSRPQLPPQPNDSQLKQALGLGIGIWDYMEKNQRELGDTFTLK
                     LPGQGPMVWTADQDVIRDVLKLREDQYDASLVQLPVDVGEQNTVFLNEKEHQDARKLI
                     IPSFTTNLLKNRAGVMQEIVEAHINRLHPGERINLPRFVGDITLDIICYTLLGLRDGA
                     RKERYKELMLNWLLEATSDVNFTLGATVGARRYRKFLNNQYLRRTEKGQMGNGKKGLL
                     PWKRAVDLKVQLAALLREDIRAIRARNAADPAGCANESHVLSLLARATDADGQPLGEE
                     RLISESIGLLIAGHETSAATAAWFMVWLQQKPAVYRKIREEVVASIAHEGELNAVKIS
                     ELPYLTACLNESQRLTPSAIGFIRWLRQDTQLGHLFLPAGTAVLPNIYLTQRDKKIYG
                     PDALEYRPERWLEEGKKFGPSEFLPFGGGRRACVGMNQGRQQLRIIFAELARRVEFSS
                     EFQNTNKLPRSRMIGGQTEPEKGVWLTVKEVRPAQYGFAVLAKTA"
     gene            complement(1933..>2410)
                     /locus_tag="VFX11_00730"
     CDS             complement(1933..>2410)
                     /locus_tag="VFX11_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEX4937169.1"
                     /translation="RHHTVISLLEQVGTVDAIICTAGNLHFGPLTEMTAAQFNLGLQD
                     KLLGQVQLALAGQHHLTEGGSITLTSGILSQEPIRNGANATAVNAALEGFVAAAAIEL
                     PKGIRINVVSPTLLSESIPTYGPFFPGFETVPAQRVGLAYQRSVEGGQTGRVYRVQ"
BASE COUNT          438 a          747 c          707 g          518 t
ORIGIN      
        1 ggacacgccg tgaacccatc cctgggggct gctccgcggc atccctgccg cagagcgtcc
       61 agattaaaga gtcccctcgc ttcccgctcc gtagacatca ttctgtgcca ccaaaaaatt
      121 cacgccgtct tcgccagcac tgcaaatccg tactgcgccg gccgcacttc cttcaccgtc
      181 agccagacgc ctttttccgg ttccgtctga ccaccgatca tgcgcgagcg cggcagtttg
      241 ttggtgttct ggaattccga ggaaaattcc acccgccgcg ccagttccgc gaaaatgatg
      301 cgcagttgct gacggccctg gttcatgccg acgcaggcac gacgtccgcc accaaacggt
      361 agaaactccg acgggccgaa tttcttgcct tcctccagcc agcgttccgg gcggtattcc
      421 agcgcatcgg ggccgtagat ttttttatcg cgctgggtga ggtaaatatt cggcagcacg
      481 gccgtgcccg ccggtaaaaa caggtggccc agttgcgtgt cctgccgcag ccagcggata
      541 aagccgatgg cggaaggcgt cagccgttgc gattcgttca ggcaggcggt gagatagggc
      601 agctcggaaa ttttcaccgc gttgagttcg ccttcgtgcg caatcgacgc caccacttcc
      661 tcgcggatct tgcggtacac cgccggtttc tgctgcagcc acaccatgaa ccaggcggca
      721 gtggcggcac tggtttcgtg gcccgcaatc agcaggccaa tgctttcgga aatcagccgt
      781 tcttcgccca gcggctggcc atccgcatcg gtggcgcggg ccagcagcga cagcacatga
      841 ctttcattgg cgcagccggc gggatcggcc gcattgcgcg cgcggatggc acgaatatct
      901 tcccgcagca gcgccgccag ttgcactttc agatccaccg cgcgcttcca cggcagcagg
      961 ccttttttgc cattgcccat ctggcctttt tccgtgcgcc gcagatactg gttgttgaga
     1021 aacttgcgat aacgccgcgc accgaccgtc gcgcccagcg taaaattgac atcgctggtg
     1081 gcttccagca gccagttcag catcagttcc ttgtagcgtt ccttgcgtgc accgtcccgc
     1141 aggcccagca gggtgtagca gatgatgtcc agcgtgatgt caccgacaaa gcgcggcagg
     1201 ttgatgcgct caccgggatg cagccggttg atatgcgcct cgacgatttc ctgcatcacg
     1261 ccggcgcggt ttttcagcag gttggtggtg aacgacggaa tgatcagctt gcgcgcgtcc
     1321 tggtgttcct tctcgttgag gaacacagtg ttctgctcgc ccacatccac cggcaactgc
     1381 accagcgacg cgtcgtactg gtcctcgcgc aacttgagca cgtcgcggat cacatcctgg
     1441 tccgccgtcc acaccatggg gccctgaccc ggcagcttca gggtgaaagt gtcccccagt
     1501 tcgcgctggt tcttctccat gtaatcccag atgccgatac ccaggcccag ggcctgtttc
     1561 agctggctgt cgttgggttg cggcggcagt tggggacgcg acgtcatggg ggactccttt
     1621 tttattatgg acagttggtt atgggcagct tttcaggggc agttcgtttg aatagaagag
     1681 atgactgctg cgtcagtata cggcgtatac ttattgattc attgagctgg atcaggaatc
     1741 agaagaaaac cctgttcaca tgaggggttt gtagccgcgc cccgcgcggc cactcgtcag
     1801 taccgtgggt gaatcacgca cgttgtgtcg ccaacaaggg gctatcgggg gcgcggagga
     1861 ggatcaggta tgatgttgag gggcgtcgca cccgagcggg ttacactccg gcccgcattt
     1921 catctcaaga ttttattgca cccgatatac ccgcccggtt tgcccgccct ccacactgcg
     1981 ttgataggcc aggcccaccc gctgcgccgg cacggtttca aatcccggaa agaatgggcc
     2041 gtaagtcgga atggattccg acagcagcgt cgggctgacc acattgatcc ggatgccctt
     2101 tggcagttcg atggccgccg ctgccacaaa tccttccaga gctgcattta ctgcggtcgc
     2161 gttggcgccg ttgcgaatcg gctcctggct cagaatgccg ctggtcaggg tgatggagcc
     2221 accttcggtc agatggtgct gtcccgccag cgccagttgc acctgcccca gcagcttgtc
     2281 ctgcaggccc agattgaatt gggcggcggt catctccgta agcggtccga aatgcaggtt
     2341 acccgccgtg cagataatgg catccaccgt gccgacctgt tccagcaggc tgatcaccgt
     2401 atgatgacgg
//
LOCUS       DASUEG010000044         5044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_329480, whole genome shotgun sequence.
ACCESSION   DASUEG010000044 DASUEG010000000
VERSION     DASUEG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329480"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VFX11_00735"
     CDS             complement(<1..1182)
                     /locus_tag="VFX11_00735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937170.1"
                     /translation="MVDEAVRAEIVSRDREVAEIERLRDERRIGLVFSPLLEEVYRLD
                     YQQRAASAFRYGSILVFMLYVLLGTGIYVFMPDQKASSWLPLYLGIGTSILVAGGLSH
                     FRQLNSWFSVYMSACGFLCIALAVAANGVVVNAEASQLVQAAIIYTVVIIYGVVGLRF
                     QHAMLAGWAGGMAGVALSSLLGGSLTWEILIRTYMGSSILGMFMAYYAERRDREMFLQ
                     SRLIQIAKWRSERYVEELDKLSRQDALTGLANRRHFDESLEQEWRRAGRQKTALAVLM
                     IDVDNFKHFNDKLGHPEGDRCLKRVAKLLSEHARRPGELVARFGGEEFVMVFPDTDHQ
                     KAIHLAEHVLESIRMARIPQPEGLERWYVTVSIGVSSTIPSERLTASRLLVAADQALY
                     AV"
     gene            complement(1363..2007)
                     /locus_tag="VFX11_00740"
     CDS             complement(1363..2007)
                     /locus_tag="VFX11_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4937171.1"
                     /translation="MAFRIIPVTPFEQNCTLIWCDETMEAAVVDPGGDLDRIRAAVKA
                     AGVTLTQILLTHAHIDHAGGVAELAEQEHLPIVGPHEGDLFWINLLDEQSRRFGFKPS
                     RPFVPTRWLHQGDTVQVGKLTLEVRHCPGHTPGHVVFFEPASRLAIVGDVLFAGSIGR
                     TDFPGGDFQTLVDAIRTQLFTLGDDVRFIPGHGPMSTIGQERATNPFVADHRFG"
     gene            2213..3319
                     /locus_tag="VFX11_00745"
     CDS             2213..3319
                     /locus_tag="VFX11_00745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4937172.1"
                     /translation="MNQLFWESRRVENFRHMDRLFPAHPLAASTTPHVFPRAEMPAPL
                     PTTYIFNDSHRLTEAFLEKTRTTGLLVLRDGVILHEEYRRDANASSTLTSWSMAKSVV
                     ATLVAIALKEGTLHSLDDTVGQYVPELKNSAWAPVRIRDLLRMASGIRFDETYDNRLA
                     DIHRIFYKTFIFNRSIDSAVAGYPAEDVPGTRFHYKSVDTQVLSLVLRRATGRPLTTY
                     AQEKLWQPLGMQDTGFWNTDFHGNELGYCCLNISLRDYAKLGQLYLQQGQWNGQQLLP
                     ADWVREATKRPEPWLAAGNGYAERGYGYHWWVPRDPDQEFFANGVWGQTIFVDEKTRT
                     VIVKTSVDPNFKANTAEMIAFMRGMVKGLTPTPP"
     gene            complement(3316..3999)
                     /locus_tag="VFX11_00750"
     CDS             complement(3316..3999)
                     /locus_tag="VFX11_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937173.1"
                     /translation="MPAPDKLPVVPLVPAPWQLKGQGYILAIRLPDAFLDHQSFIADS
                     QRHTRRGRVAYVMFVDYRESDVGPYHELLYIPGTLAFGRRRHLSISKIYVSTRDSVVN
                     GHANWGIPKERCDFQVRYGVEGVDEVRLLRDGQVIAELDLRPRLFRVPFDGRLVPARF
                     RTLAQHFEGREFTYIPVAKGHVRPASLVRSRFDSRHFPDISQGKVVACVKVTDFDMTF
                     PVATVRMLD"
     gene            complement(4054..4401)
                     /locus_tag="VFX11_00755"
     CDS             complement(4054..4401)
                     /locus_tag="VFX11_00755"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4937174.1"
                     /translation="MAICEPLEQDARALLEAEARTQLSLFDLQGWQLLQEGGCWHLLK
                     TYPTSNFVQSLALAQRVGWLAEQEGHHPELTIAWGRCTVAWWSTRLNGLHENDFRMAA
                     ATDELLYSGRELS"
     gene            complement(4401..>5044)
                     /locus_tag="VFX11_00760"
     CDS             complement(4401..>5044)
                     /locus_tag="VFX11_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937175.1"
                     /translation="SLVLAGCASGPATVKTGFYHSPARAYSLALDSGVFRGGVTLTEK
                     CDANGGTLNLWDETNRFFRVDYLKLNKHPVAVIPSFASERTIDEMVLNGYQREVLAKA
                     PGISRYEVMLSEFVDTGRGDAMFSVASLEMKDEAMPSDVAARNFFYGILVFTRGDFVY
                     VLQHRFDAYQPDKLKNLLSALRQDMLVPGLLRHNESVKSVQSAARQPAAPDAC"
BASE COUNT         1182 a         1613 c         1340 g          909 t
ORIGIN      
        1 gacggcatac aaagcctggt ccgccgccac cagcaaccgc gacgccgtca gccgctcaga
       61 cgggatggtc gacgacacac cgatgctgac ggtgacatac caacgctcca atccttccgg
      121 ctgaggaatg cgcgccatgc gaatgctttc gagaacatgc tcggccaggt gaatcgcctt
      181 ctggtgatcg gtatccggaa acaccatcac gaactcctca ccaccaaacc gggccaccag
      241 ctcccccgga cgacgagcat gctcagagag caatttggca acccgcttca ggcagcgatc
      301 accctccgga tgtcccagct tgtcattgaa gtgcttgaag ttgtcgacat caatcatcag
      361 cacggccaac gcagtcttct gacgaccggc acgacgccac tcctgctcga gtgactcgtc
      421 aaaatggcga cggttagcca accccgtcag cgcatcctgt cgcgacagct tgtcgagttc
      481 ttcgacataa cgttcgctgc gccatttcgc aatctggatc agtcgtgact gcaaaaacat
      541 ctcgcgatca cgacgctccg cgtaataggc catgaacatg ccaagaatac tgctgcccat
      601 gtaggtgcgg atcaggattt cccacgtcag gctacccccc agcaagctgc tcaatgcgac
      661 ccctgccatg cctcccgccc agccggccag catggcatgc tgaaaccgca atccgaccac
      721 cccatagatg atgacaaccg tataaatgat ggcggcctgc accagctgac tagcctcggc
      781 atttaccact acgccattcg ccgccaccgc cagcgcaatg caaagaaatc cgcatgcact
      841 catgtaaacg ctgaaccagc tattgagctg gcgaaaatgg gacaagccgc cagccaccag
      901 gatggacgtg ccgataccga gatagagagg cagccaacta gaggccttct gatccggcat
      961 gaagacgtat atcccggtgc ctagcaggac atacagcatg aacaccagaa tggagccgta
     1021 acgaaatgcc gacgcggccc gttgctgata gtcaaggcga taaacctcct ccagcaacgg
     1081 actgaacacc agtccgatac gacgttcgtc gcgcagccgc tcaatttcgg ccacctcgcg
     1141 atcacgcgac acgatctctg cacgaaccgc ttcatccacc ataatccttt acccgcctac
     1201 tgctgactgg cctgatcctg tcctcagcct agaacacccc cttcctttgg acagtgaaca
     1261 aagtgcgtta ccgacaaacg caggataaac ggagccgagc agcagaatcc gtcgacgaca
     1321 tcacgccgga tgccaacata aaggcataag cgtcgtagcg gcttagccga agcggtgatc
     1381 ggccacaaag ggattcgtgg cccgctcctg gccgatggtg gacatggggc catgccccgg
     1441 aatgaagcgc acatcatctc ccagcgtgaa cagctgagta cgaatggcat caaccagtgt
     1501 ctggaaatcg ccaccgggga aatccgttcg accgatggag ccggcaaaca gtacgtcacc
     1561 cacgattgcc aggcgcgagg ctggctcgaa aaacacgaca tgccctgggg tatgccccgg
     1621 acaatgccgc acctccagcg taagcttgcc gacctgaacg gtatccccct gatgcagcca
     1681 acgggttggc acgaacggac gcgacggttt gaagccaaaa cggcgcgatt gttcatccag
     1741 gagattgatc cagaacagat ctccctcatg cggccccaca atcggcaaat gctcctgttc
     1801 agccagctca gccactcccc ccgcatggtc aatgtgggca tgtgtgagaa gaatctgcgt
     1861 aagtgtcaca ccggccgcct tgacagcagc tcgaatgcgg tcaaggtccc cgcccggatc
     1921 cactactgcc gcctccatgg tctcgtcaca ccagataagc gtgcagttct gttcaaacgg
     1981 ggtgacagga atgatgcgaa aagccatgag cgtctcggga gggcagtgac aaaggagagt
     2041 tgtgtgaatt atgcccttga ccccgctgcc ccgcacccct ttactcggaa agtcgcgata
     2101 acaagaacac accgccatgc gcctgcgcac tgccctgctt ctgttgacgt ccgtcatcat
     2161 cgcacttggc cttttcattg cccggccttg gtctccgtgg tccccctggg ccatgaacca
     2221 gctgttctgg gagtcccgcc gggtcgagaa ctttcggcac atggaccggc tttttcctgc
     2281 tcacccgctg gccgccagca caaccccaca tgtgtttccc cgggcagaga tgcctgcgcc
     2341 actgcccacc acctacattt tcaatgacag ccatcgactg acagaggctt ttctggagaa
     2401 aacccgcacg accggactgc tggtcctgag agatggcgtg atcctccacg aagaataccg
     2461 gcgtgacgcc aatgccagct cgacactgac gtcatggtcg atggccaaaa gcgtggtcgc
     2521 caccctcgtc gccatcgccc tgaaagaggg aaccctccac tcgctggatg acaccgtggg
     2581 tcagtatgtt ccggagctaa agaacagcgc atgggctccc gttcgcatcc gcgacctgct
     2641 gcgcatggcc agcggcatcc gcttcgacga aacctatgac aaccggctcg ccgacattca
     2701 ccgtatcttc tacaaaacct ttatcttcaa ccgatccatc gacagcgctg ttgccggcta
     2761 tcctgccgaa gatgtccccg gcacccgctt ccactacaag agcgtggaca cgcaggtgct
     2821 gtcgctggtc ctgcgccgcg ccaccggccg cccgctaacc acctatgcgc aggaaaagct
     2881 ttggcaaccg cttggcatgc aggatacggg cttctggaat acagacttcc atggcaacga
     2941 actgggttac tgctgtctga acatctccct gcgtgattat gcaaagctgg gacagctcta
     3001 cttgcagcaa gggcaatgga atgggcaaca actactgcca gctgactggg tacgcgaagc
     3061 caccaaacgc cccgagccct ggctggctgc tggcaatggc tacgccgaac gcggttacgg
     3121 ctaccactgg tgggtacccc gcgatcccga tcaggaattc ttcgccaatg gcgtgtgggg
     3181 ccagacgata ttcgtggacg agaaaacccg gaccgttatc gtgaagacca gtgtcgaccc
     3241 caacttcaag gccaacacgg cagagatgat cgcttttatg cgtggcatgg tgaagggact
     3301 cacccccaca cccccttaat ccagcatccg caccgtggcc accggaaagg tcatgtcaaa
     3361 atcggtcacc tttacacagg ccaccacctt gccctgactg atgtccggaa aatggcggct
     3421 gtcgaaacgc gaacgtacca gcgatgcagg gcggacatgc cccttggcca ccggaatgta
     3481 ggtaaattca cgcccctcaa agtgctgggc caacgtacga aagcgcgccg gaacaagacg
     3541 gccgtcaaag ggcacacgga acaggcgcgg tcgcaggtcc agttccgcaa tcacctgacc
     3601 atcccgcaac agacgcactt catccacacc ctctacgcca taacgcacct gaaagtcgca
     3661 gcgctccttg ggaatgcccc agtttgcgtg accattgacg accgagtccc gggtcgacac
     3721 ataaatcttg ctgatggaaa ggtggcgccg gcggccgaag gccaatgtac cgggaatgta
     3781 gagcagctcg tgatagggcc cgacgtccga ttcgcggtaa tcgacaaaca tcacataggc
     3841 cacccggccc ctgcgggtat gacgctggct gtcagcaatg aaagactggt gatccagaaa
     3901 ggcatccggc aagcggatgg caagaatgta accctgacct ttgagttgcc agggcgcagg
     3961 caccaggggg acaacaggaa gtttgtcagg cgcaggcatt gcggctcctt gtgaccaatc
     4021 ggtcaaggcc tgcagtgtag ccttcgttct gcttcaggac aattcacgcc cgctgtaaag
     4081 caattcgtct gttgcagccg ccatgcggaa gtcattttca tgcaggccgt tgagtcgcgt
     4141 cgaccaccag gccacggtgc aacggcccca ggcaatggtc agctcgggat gatgaccttc
     4201 ctgctcggcc aaccagccga cacgttgcgc cagcgccagc gactgcacga aattcgaggt
     4261 cggataagtt ttaagcagat gccagcagcc gccttcctga agcagctgcc atccctgcag
     4321 atcaaacagg gacagttgcg tacgggcctc tgcctccagc agcgcccggg catcctgctc
     4381 cagcggttcg cagatcgcca tcagcaggcg tccggagcag caggctgccg ggcggcagac
     4441 tgcaccgact tcacgctttc attgtgccgc aacaatcccg gcaccagcat gtcctggcgc
     4501 agcgccgaca agaggttctt cagcttgtcc ggctgatagg catcgaaacg gtgctgcagt
     4561 acatagacaa agtcaccgcg cgtgaacacc agaatgccgt aaaagaagtt gcgcgccgcc
     4621 acgtcggaag gcatcgcctc atccttcatt tccaggctgg ccacgctgaa catggcatca
     4681 ccacgtcctg tatccacaaa ctccgacagc attacctcat aacggctgat gccgggtgcc
     4741 ttggccagta cctcgcgctg ataaccgttc aacaccattt catcgatggt gcgttcgctc
     4801 gcgaagctgg ggatgaccgc aacaggatgc ttgttgagct tcaggtaatc cacacgaaaa
     4861 aagcggttgg tttcatccca cagattaagc gtaccgccgt tggcatcgca cttttccgtc
     4921 agggtcacgc caccgcggaa aacaccggag tccaaagcaa gggaataggc acgggcaggc
     4981 gaatgataaa agcctgtctt cacagtggca ggaccagagg cacagcccgc cagcaccaga
     5041 gagg
//
LOCUS       DASUEG010000045         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2008134, whole genome shotgun sequence.
ACCESSION   DASUEG010000045 DASUEG010000000
VERSION     DASUEG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008134"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1728)
                     /locus_tag="VFX11_00765"
     CDS             complement(148..1728)
                     /locus_tag="VFX11_00765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX4937176.1"
                     /translation="MKKSPETALSDKSYVLAIDNGTQSVRALIFDLKGQLIAKSKVPL
                     EPYFSVNPGWAEQHPQYFWDNLCRACQQLWDMIDFPRSAIKAVALTTQRATVVNLDKN
                     GEPLRPAIIWLDQRRAENLPAMSGAWDKLIKLSGNGDTLNYFRAQAECNWIAQQQPEI
                     WNNTHKYLLLSGYHIHKLTGRYADSTAAQVGYIPYDFKRFAWAGGADWKWQALAVRRQ
                     QLPDLVPPGKLIGHISADAALATGIPAGLPLISAGADKACEVIGSGCLTPDMGSLSYG
                     TTATFNTTNPKYIETIPMVPPYPAAIPNAYSTEIIVQRGYWMVNWFRREFGQREELQA
                     KERGIEPEQLFDELLKEVPAGSMGLMLQPYWSPGVKQPPEAKGAIIGFGDVHTRAHIY
                     RAIIEGLAYALREGREKVEQRSKVKVTTLRVSGGGSQSDEAMQITADIFGMPAERPHT
                     YETSGLGAAINAAVGVGLYPDHATAIRNMTHRGDVFHPIEANRRLYNQLYHEVYQKMY
                     DNLSPLYRAIRRITGYPK"
     gene            complement(1725..>2385)
                     /locus_tag="VFX11_00770"
     CDS             complement(1725..>2385)
                     /locus_tag="VFX11_00770"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4937177.1"
                     /translation="LQLAQQAFPGANVTAADVISSYAGVRPVISKNRSKDPSKEKRDH
                     QVWVDKGLVSVSGGKLTTFRLIARDTLQAAKPFLQGVEFRNQDEQLFDYPIATPHALG
                     LQDKAWAQRMIGRYGDQVKSLLQDAPESERNKIAGTEFCLAELRWSCRNEQIVHLDDL
                     LLRRTRLGLLLHNGGAEILPHVKEICQQELDWNQDRWHSEEQRYLSLWRHYYFLPDAT
                     A"
BASE COUNT          424 a          689 c          724 g          548 t
ORIGIN      
        1 gctgaaaaac atcgaaacga aaattgccga attgcagcgt atgcacaagg cgctgcagcg
       61 gatcaccgac cagtgttgcg gcggaccgga gccggcgatc cactgttcca ttctggatgc
      121 gctggagcag taacattttt acctgcgtta tttcggataa cccgtaatgc ggcgaatggc
      181 gcggtacagc ggactgaggt tgtcatacat tttctgatac acctcatggt acagctgatt
      241 gtacaggcgg cggttcgctt caatcggatg aaatacgtcg ccccggtgtg tcatgttgcg
      301 gatggcggtg gcgtgatccg gatacaggcc cacgcccacg gcggcgttga ttgccgcacc
      361 cagccctgac gtttcgtagg tgtgcggacg ttccgccggc atcccgaaaa tgtccgccgt
      421 gatctgcatc gcttcatcgc tctgtgagcc gccaccggac acccgcagtg tcgtcacttt
      481 caccttgctg cgctgctcca ctttttccct gccttcgcgc agtgcgtaag ccagtccttc
      541 gataatggca cggtaaatgt gggcgcgcgt gtgcacgtcg ccgaagccga tgatggcgcc
      601 cttcgcttct ggcggctgtt tcacgccggg ggaccagtaa ggttgcagca tcaatcccat
      661 cgacccggcc ggcacttcct tcagcaattc atcaaataac tgttccggtt caatgccgcg
      721 ttccttcgcc tgcaattcct cgcgctggcc gaattcgcgg cggaaccagt tcaccatcca
      781 gtacccgcgc tgcacgatga tttccgtgct gtaggcgttg ggaatggccg ccgggtaggg
      841 cggcaccatg ggaatggttt cgatgtattt cggattggtg gtgttgaacg tggcggtggt
      901 gccgtagctg aggctgccca tgtccggtgt caggcagccg gagccaatca cctcgcaggc
      961 cttgtcggcg ccggcggata tcaagggcag gccggcagga atgccggtgg ccagtgcggc
     1021 gtctgcgctg atgtgaccga tcagcttgcc ggggggcacc agatctggca gttgctggcg
     1081 tcgcacagcc agtgcctgcc atttccagtc ggcaccaccg gcccaggcaa aacgcttgaa
     1141 atcgtagggt atatacccca cttgcgcggc ggtggaatcg gcatagcgtc cagtgagttt
     1201 gtgaatgtgg tagccggaca gcagcagata tttgtgcgtg ttgttccaga tttccggctg
     1261 ctgttgtgcg atccagttgc attcggcctg ggcccgaaaa taattcagtg tgtcgccgtt
     1321 gccgctcagc ttgatcagct tgtcccaggc gccgctcatg gctggcaggt tctccgcgcg
     1381 acgctggtcc agccagatga tggcggggcg cagtggttcc ccgtttttat ccagattcac
     1441 cacggtggcg cgctgggtgg tgagcgccac ggccttgatg gcactgcggg gaaaatcgat
     1501 catgtcccac aattgctggc aggcacggca caggttgtcc cagaaatact gcggatgctg
     1561 ttccgcccag ccgggattga cggaaaaata cggctccagc ggcaccttgc ttttggcgat
     1621 cagctggcct ttcagatcga atatcagcgc gcgcacgctc tgggtgccgt tgtcgatggc
     1681 gagcacgtag cttttgtcgg aaagtgctgt ttccggcgat tttttcatgc agttgcatcc
     1741 ggcagaaaat agtaatgtcg ccacagggac aggtagcgtt gctcctcgct gtgccagcgg
     1801 tcctggttcc agtccagttc ctgctggcag atctctttca catgcggcag gatttccgcg
     1861 ccgccattgt gcaacagcag ccccagccgg gtgcggcgca acagcagatc gtccagatgc
     1921 acgatctgtt cattgcggca cgaccagcgc agttccgcca ggcagaattc agtgccggcg
     1981 attttgttgc gttcgctttc cggtgcgtcc tgcagcagtg atttcacctg atcgccgtag
     2041 cggccgatca tccgttgcgc ccaggctttg tcctgcaggc ccagtgcatg cggggtggcg
     2101 atggggtagt cgaacagctg ctcgtcctga ttgcgaaatt ccacgccctg caaaaaaggt
     2161 tttgccgcct gcaacgtgtc gcgcgcgatc agccggaaag tagtgagttt gccgccgctg
     2221 acactgacca gtcccttgtc gacccacacc tgatgatcgc gtttttcttt cgagggatct
     2281 ttgctgcggt tcttgctgat tactgggcgc acgccggcat aacttgaaat tacatcggcg
     2341 gccgtgacgt tggcgccagg aaatgcctgc tgcgccaatt gcagc
//
LOCUS       DASUEG010000046         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_394906, whole genome shotgun sequence.
ACCESSION   DASUEG010000046 DASUEG010000000
VERSION     DASUEG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394906"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1417
                     /locus_tag="VFX11_00775"
     CDS             275..1417
                     /locus_tag="VFX11_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4937178.1"
                     /translation="MSGARQDDEFSRDLMEDFFLDFREAHQSCENTLIDLEHDPANTE
                     LLNTLFRSVHTIKGNLVYVGLKDLTPLIQSVEDVLDAVRKHSLTYDASLSDVIMLAMD
                     KTKQMVEARMAQQPLPLDELALNQLCQQISRVAEVSLTDRPAVVRAVLAQMDPTILIE
                     PVAAPRPEQPAPLALQPEENEILQRFGIPLGDDIRFLQHLIQPLEERSHYWKGRNARL
                     LQLALAMNRHAGEPVEPDQLAVAVFLHDLGMAFLPISILHKTGALSAADLEAVRQHPL
                     VATQLIASLKHWEQAGLIVKEHHEREDGQGYPAGLKGAAICPGAKILAIADTFDACTH
                     ERAYSAAIKRPLVRAVLEINRCSGSHFDAEWVEVFNEVARRLQNQA"
     gene            1605..2051
                     /locus_tag="VFX11_00780"
     CDS             1605..2051
                     /locus_tag="VFX11_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX4937179.1"
                     /translation="MPAKKKATAKPAAKKPAAKKPAAKKAPAKKAAAPKAAAAPKKIT
                     TVTEAMTKSQIMTTIADATQLSRKQVQAVFDELSSLIEAHVKKKGAGQFTVPGLLKLK
                     VIDKPATKARKGINPFTGEETTFKAKPASRKVKAVALAKLKEMVNS"
BASE COUNT          520 a          616 c          607 g          350 t
ORIGIN      
        1 gccacaggac tgacactcat actcatagat cggcatggtg gtaaagcctc caactctcgt
       61 gctcaaacca ttgaaaaaac gcggctttgg ccggcctgcg catcaggaat agccgggcaa
      121 ggccaatgac gggtggaacc ggcaccgggg gccgggccgg aaaaagggcc acattataac
      181 gggttgcccg gcttttagca gtatgggccg gggcgcccct gtggcaggcg accactatac
      241 tgaaagaatc gacggcaagt gcgggagaaa gaccatgagc ggcgccagac aggacgacga
      301 attcagccgg gacttgatgg aggatttctt tctggatttt cgcgaggcgc accagtcctg
      361 cgaaaacacc ctgatcgatc tggagcatga tcccgccaat accgagctgc tcaacaccct
      421 gttccgctcg gtgcacacca tcaagggcaa tctggtctac gtggggctga aggatctgac
      481 gcctctgatc cagagcgtgg aggatgtgct ggatgcagtg cgcaaacaca gcctgaccta
      541 cgacgcatcc ctcagtgacg tgatcatgct ggcgatggac aaaaccaagc agatggtgga
      601 agcgcggatg gcgcaacagc cgctgccact ggacgagctt gcgctgaacc agctgtgcca
      661 gcagatcagc cgggtggcgg aagtgagcct gacagaccgg ccggccgtgg tgcgcgccgt
      721 gctggcacag atggacccga cgattctgat cgaacccgtc gccgccccac gccccgaaca
      781 acctgcaccg ctggcactgc agccggagga gaacgaaatc ctgcagcgct tcggcatccc
      841 cctgggtgac gacattcgtt tcctgcaaca tttaatacag ccgctggagg aacgctccca
      901 ctactggaag ggacgcaacg cccgcctgct gcaactggcg ctggccatga accggcatgc
      961 aggcgaaccg gtggaaccgg atcagctggc ggtggcagta tttctgcacg atctcggcat
     1021 ggcctttctg cctatcagca tcctgcacaa gaccggcgca ttgtcggcgg cggacctgga
     1081 ggctgtgcgt cagcatccgc tggtcgcgac gcagctgata gcttcactga aacattggga
     1141 gcaagccggg ctgatcgtga aggaacatca cgaacgggag gatggccagg gttatcccgc
     1201 cggattgaaa ggtgccgcca tctgtcccgg cgcgaaaatc ctggcgatcg cagacacgtt
     1261 cgacgcctgc acgcacgaac gcgcatacag cgcggcgatc aagcgcccgc tggtgcgtgc
     1321 ggtgctggaa atcaaccgct gttcaggctc acatttcgac gcggaatggg tcgaagtctt
     1381 caacgaggtg gcgcgcagac tgcaaaatca ggcctgattt tcaatttttt tgcagtaaac
     1441 ctcgaaaaaa ccgggaaaaa tcgcgtttta tcacttgcgg gcgccaatat taggatcaat
     1501 aattacccgc gactgtccgg gccggctggc gtaatccacc gcggaaaaac agcggtggaa
     1561 tgacgaaagt cgccgggcga taactagaaa taaaggaagc aatcatgcct gcaaagaaga
     1621 aagccaccgc aaaacctgct gccaaaaagc ccgctgccaa aaaacctgca gcgaaaaaag
     1681 cgcccgccaa gaaagctgct gctcccaagg ctgctgctgc gccgaaaaaa atcaccaccg
     1741 ttactgaagc gatgaccaag tcccagatca tgaccaccat cgccgacgca acccagcttt
     1801 cccgcaagca agttcaggct gttttcgacg agctgtcttc cctgattgaa gctcacgtga
     1861 agaaaaaggg cgctggccag ttcaccgttc ccggcctgct gaagctgaaa gtgatcgaca
     1921 agcctgccac caaggcacgc aagggcatca accccttcac tggcgaagaa accacgttca
     1981 aagccaagcc cgccagccgc aaagtaaaag cggttgccct ggccaagctg aaagagatgg
     2041 tcaacagctg attgccattc acacgggcaa tgtgaaaacg ggggctgcgg ccc
//
LOCUS       DASUEG010000047         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1853548, whole genome shotgun sequence.
ACCESSION   DASUEG010000047 DASUEG010000000
VERSION     DASUEG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853548"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /gene="rplL"
                     /locus_tag="VFX11_00785"
     CDS             <1..351
                     /gene="rplL"
                     /locus_tag="VFX11_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX4937180.1"
                     /translation="LNAVAEMSVMDLVELISAFEEKFGVSAAAVAVAAGPAAGAAAAA
                     EEKTEFDVILASFGANKVGVIKVVREATGLGLKEAKDLVEAAPKAVKEGASKEEAADL
                     KKKLEEAGATVEVK"
     gene            613..>2557
                     /gene="rpoB"
                     /locus_tag="VFX11_00790"
     CDS             613..>2557
                     /gene="rpoB"
                     /locus_tag="VFX11_00790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX4937181.1"
                     /translation="MAYSYTEKKRIRKNFGKLPALMDVPYLLAIQVDSYRSFLQDGKL
                     PRQREDLGLQAAFKSVFPIVSYSGNAALEFVEYHLGTPMFDVRECVLRGVTYAAPLRV
                     KIRLMIYDKESSVKAIKDIREQEVYMGEIPLMTENGTFIINGTERVIVSQLHRSPGVF
                     FDHDKGKTHSSGKLLYNARIIPYRGSWLDFEFDPKDLVFVRIDRRRKLPATILLRALG
                     YNADEILDMFFETNTIHVDKDGFKLDLVPERLRGETALFDIAAKGKVIVEAGRRITAR
                     HIREIEKAGLTQLDVPVEYLLGKVVAKTLVNKDTGEIISECNSIVADDLLTKITKAGI
                     ASFEILYTNELDRGAFISDTLRADPTRTELDALVEIYRMMRPGEPPTKDAAENLFKNL
                     FFSAERYDLSNVGRMKFNRRLGRDSDLGEGVLSREDIVDVLRTLIDIRNGKGEVDDID
                     HLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLAESENLMPQDLVNAKPVSAAIKE
                     FFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIE
                     TPEGPNIGLINSLASFARTNDYGFLESPYRRVIDGKVTDEYEYLSAIEEAEYVVAQAD
                     SKVDKNGRLVDDLVAVRHKNEFTV"
BASE COUNT          534 a          670 c          763 g          590 t
ORIGIN      
        1 ctgaatgcag ttgctgaaat gagcgtgatg gacctggttg aactcatctc tgctttcgaa
       61 gagaagttcg gcgtgtccgc tgctgctgtt gctgtggctg ctggtcctgc tgctggcgct
      121 gctgccgctg ctgaagagaa gaccgagttt gacgtaatcc tcgcctcctt tggcgccaac
      181 aaggttggcg tgatcaaggt tgtgcgtgaa gctaccggcc tgggcctcaa ggaagccaag
      241 gacctggtgg aagctgctcc gaaggctgtc aaggaaggtg cttccaagga agaagctgct
      301 gacctcaaga agaagctgga agaagctggc gccacggtgg aagtcaagta ataatacttg
      361 gctcgacagg tgcttgactg cctgaccgga cgctgaaaag tggctggtga ctcgaatggg
      421 agtcaccggc ctttttccgt tttcatgatt gtttgttcaa acggtgccag ggcggagaag
      481 ggaattaccc ggatttgccg gcatggatgc agggaagagt ggctgccaca ggtgtctgga
      541 aaggcatctg tggccgctgc tttttaatac aggcgccaca tcaactggcc tacgtcacgc
      601 tgaggacccc agatggcata ctcatatacc gagaagaaac gtatccggaa gaatttcggc
      661 aaattgccgg ctctgatgga tgtgccctac cttctggcca ttcaggtcga ctcctatcgg
      721 agcttccttc aggacggcaa gctgccacgg cagcgcgaag accttggtct gcaggctgca
      781 ttcaagtcgg tattcccgat cgtcagctac tccggcaatg ctgcccttga gtttgtcgag
      841 tatcaccttg gcacaccgat gttcgatgtg cgtgagtgtg tactgcgcgg cgtgacctat
      901 gctgcaccgc tacgcgtcaa gatccgtctg atgatctatg acaaggagtc ctcggttaaa
      961 gccatcaagg acatccgcga gcaggaagtg tacatgggtg aaattccgct catgactgag
     1021 aacggtacct tcatcatcaa tggtaccgag cgcgtgattg tctcgcagct gcatcgttcg
     1081 cctggcgtat tctttgacca tgacaagggc aagacgcaca gctccggcaa gctgctgtat
     1141 aacgctcgca tcattcctta tcgcggctct tggttggact tcgagtttga tccgaaggac
     1201 ttggtcttcg tgcgtatcga tcgtcgtcgc aaactgcctg cgacgattct gctgcgtgcg
     1261 cttggctaca atgccgatga aatcctcgac atgttctttg agaccaatac gattcatgtc
     1321 gacaaggatg gtttcaagct tgacttggtt ccagaacgtc tgcgtggcga gacggcgctg
     1381 ttcgacattg ctgcgaaggg caaggtcatt gtcgaggcgg ggcgtcgtat tactgctcgt
     1441 cacattcgtg agatcgagaa ggcaggcttg acccagcttg atgtgccggt tgaatacctg
     1501 ctcggcaagg tggtggccaa gacgctggtg aacaaggata ccggcgaaat catctccgag
     1561 tgcaacagca tcgtggcaga cgatctgctg accaagatta ccaaggccgg catagcttcc
     1621 tttgagatcc tgtacaccaa tgaactggat cgtggcgcct tcatttccga tacgctgcgt
     1681 gccgatccga cgcgtaccga gctggatgcg ctggttgaaa tttaccgcat gatgcgtccg
     1741 ggcgagcccc ccacgaagga tgccgccgag aacctgttca agaacctgtt cttctctgca
     1801 gagcgttacg atctgtctaa cgtcggtcgc atgaagttca accgccgtct ggggcgcgac
     1861 agcgatctgg gtgagggtgt gctgtcgcgt gaagacatcg ttgatgtgct tcgtacgctg
     1921 atcgatattc gcaacggcaa gggcgaggtt gatgatatcg atcatctcgg caatcggcgt
     1981 gtgcgctccg ttggagaaat gacggaaaac cagttccgag tggggctggt gcgtgttgag
     2041 cgcgccgtga aggagcgcct gtctctggcc gagtccgaga acctgatgcc tcaggacctg
     2101 gtgaatgcca agcctgtatc ggcggccatc aaggaattct ttggctcctc gcagctctcg
     2161 cagttcatgg accagaacaa tcctctgtcg gaagtgactc acaagcggcg tgtttctgcg
     2221 cttggtccgg gcggcctgac gcgtgagcgt gccggcttcg aagtgcgtga cgtgcatcca
     2281 acccattacg gtcgtgtatg cccgattgaa acgcctgaag gtccgaacat cggtctgatc
     2341 aattctctgg cgtcttttgc tcgcaccaat gattacgggt tcctcgagtc cccgtatcgc
     2401 cgcgtgattg atggcaaggt gacggacgaa tacgagtacc tgtccgccat cgaggaggcc
     2461 gagtacgtcg tggcccaggc cgactccaag gtcgacaaga acggccgcct ggtcgacgac
     2521 ctggttgccg tgcgccacaa gaacgaattc accgtga
//
LOCUS       DASUEG010000048         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_107996, whole genome shotgun sequence.
ACCESSION   DASUEG010000048 DASUEG010000000
VERSION     DASUEG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107996"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VFX11_00795"
     CDS             <1..110
                     /locus_tag="VFX11_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX4937182.1"
                     /translation="RVMVASQKSSLAFQAVVQVRNKLVDSYKEIMNMPV"
     gene            123..1820
                     /gene="fliF"
                     /locus_tag="VFX11_00800"
     CDS             123..1820
                     /gene="fliF"
                     /locus_tag="VFX11_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX4937183.1"
                     /translation="MAEVAEKSPVAGFAVLEGLGSLSVLKQVGLMVGVAASIALGFWV
                     VLWTQEPDFRPLYTDISHLEASQVADLLQQEEIRFKVDTNSGMLLVESDRINDARMKL
                     AQEGFPAGTAMGYELLDKEQAFGTSQFMEKTRYQRSIEGELAKTVSSMNRVRGARVHL
                     AMPKRSVFINDNRKPSASVFVELYPGQTLDRMQVLAIVQLVASSIPELDEKSVTVVDQ
                     RGQLLSDNETDGELALAAKQFDYGKKLEDKYRERIAGILEPVVGPEGFKVQVSADIDF
                     NQTEQTSEYFNPDLPAVRSEQTMNEVVGAGGAVAGIPGALSNQPPGAVTVPETAAAPG
                     AAGAQQSQAASNGNSRQQSTRNFELDRTISHTRHQVGGVKRLTVAVVLDDRQVQVTGA
                     DNKIETKRQPLTDAELERIRVLVRDAIGFSAARGDSVSVINQAFHTLQPEAVLDIPPT
                     PMWEQPWFWDLAKRGAGFLLVIFLIFGVFRPILKRLAESGKEESLAALESQLGGSLGL
                     EGMQTAGGPEVTLTGTSNPLLPGPNASYEQHVDAVKTLVAEDPRRVAQVVKTWIANE"
     gene            1813..>2298
                     /locus_tag="VFX11_00805"
     CDS             1813..>2298
                     /locus_tag="VFX11_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX4937184.1"
                     /translation="MSDPNSKRDAAKGVSQLEKAAILLMSLGEDNAAEVLRHMGPKEV
                     QKLGMTMSRLQNVQKDQVEGVMAEFMDVISNQTSLGVGADGYIRKMLNNALGEDKAST
                     LIDRILTGGNTTGLDTQRWMDARSISDLIRFEHPQIQAIVIAYLDPDQGAEALRYFDE
                     RV"
BASE COUNT          502 a          616 c          734 g          446 t
ORIGIN      
        1 cccgcgtgat ggtcgcctcg cagaaatcca gcctggcgtt ccaggcggtg gtgcaggtgc
       61 gcaacaagct ggtggattcg tacaaagaaa tcatgaacat gccggtgtaa cggagctgaa
      121 ccatggcaga agtcgctgaa aaaagcccgg tagccggatt tgcggtgctg gaagggctgg
      181 gcagcctgag cgtgctcaaa caggttggtt tgatggtggg cgtggccgcg agcattgccc
      241 tgggattctg ggtggtgttg tggacgcagg agcctgattt tcgcccgctg tacactgata
      301 tttcccacct ggaggccagc caggtggcgg atctgctgca gcaggaggag atccggttca
      361 aggttgatac caacagcggc atgttgctgg tggaaagcga ccgtatcaat gatgcccgca
      421 tgaagctggc gcaggaaggc tttcctgccg gcacggccat gggttacgaa ctgctcgaca
      481 aggagcaggc atttggcacc agccagttca tggagaaaac ccgctaccag cgcagcattg
      541 aaggcgagct ggcgaaaacc gtgtccagca tgaatcgcgt gcggggtgcg cgcgtacatc
      601 tggcgatgcc gaaacggtct gttttcatta acgacaatcg caaacccagc gcatccgtgt
      661 ttgtcgagct ctatccgggg cagaccctgg atcgcatgca ggtgctggcg attgtgcaac
      721 tggtagcgtc gagcattccc gagctggatg aaaaatccgt cacggtggtc gaccagcgtg
      781 gtcagttgct gtcggataac gaaacagacg gcgagctggc actggccgca aagcagttcg
      841 actatggcaa aaaactggaa gacaaatacc gtgagcgcat cgctggcatt ctggagccgg
      901 tggtgggtcc tgaaggcttc aaggtgcagg tcagcgccga catcgatttc aatcaaaccg
      961 aacagacatc ggaatatttc aatccggatc tgcccgccgt gcgcagcgag caaaccatga
     1021 atgaagtggt gggtgccggc ggcgcggttg ccggcattcc gggcgcgctg tccaatcagc
     1081 caccgggtgc ggtaaccgtg ccggaaacgg ccgcagcccc cggtgccgcc ggtgcacagc
     1141 agtcgcaggc cgccagcaat ggcaacagcc gtcagcaatc cacccgcaat ttcgagctcg
     1201 accgcaccat cagtcatacc cgtcatcagg tcggtggcgt caagcgtctg acagtggcag
     1261 tggttctgga tgacaggcag gtgcaagtta ctggcgccga taacaaaatc gaaaccaagc
     1321 ggcagccgct cacggatgcc gagctggagc gcattcgggt gctggtacgc gatgccattg
     1381 gattcagtgc agcacgtggc gacagtgtga gtgtgatcaa ccaggctttt cacaccctgc
     1441 aacctgaagc cgttttggat attcccccga cgcccatgtg ggaacagccc tggttctggg
     1501 atctggccaa gcggggcgcc ggtttcctgc tggttatctt cctgatcttt ggtgtattcc
     1561 ggcctatact gaaaagactg gcggaatccg ggaaggaaga atcgctggcg gcgcttgaat
     1621 cgcagttggg cggctcgctg ggcctggaag gaatgcagac cgctggtggt ccggaagtaa
     1681 ccctcaccgg cacgtccaat ccgttgctgc cgggtccgaa cgcatcgtac gaacaacacg
     1741 tggatgctgt gaaaaccctg gtggcggaag atccgcgccg cgtggcgcag gtagtgaaga
     1801 cctggatagc gaatgagtga tcccaattcc aagagagatg ctgccaaagg cgtctcccag
     1861 cttgagaagg ccgcgattct gctgatgtcc ctcggtgagg acaatgcggc ggaagtgttg
     1921 cgtcacatgg ggccgaagga agtacagaaa cttggcatga cgatgtcgcg gctgcagaac
     1981 gtgcagaaag accaggttga aggcgtgatg gcggaattca tggacgtgat ttccaaccag
     2041 acctcgctgg gtgtaggcgc cgatggttat atccgcaaga tgctgaacaa tgcgctgggt
     2101 gaagacaagg ccagcacgct gatcgaccgt attctcaccg gtggcaacac caccggcctg
     2161 gatacccagc gctggatgga tgcgcgttcc atttccgatc tcatccgttt tgagcatccg
     2221 cagatccagg ccatcgtcat cgcgtatctc gatcctgatc agggcgccga ggcgctgcgt
     2281 tatttcgatg agcgcgtg
//
LOCUS       DASUEG010000049         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1632347, whole genome shotgun sequence.
ACCESSION   DASUEG010000049 DASUEG010000000
VERSION     DASUEG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632347"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFX11_00810"
     CDS             complement(<1..441)
                     /locus_tag="VFX11_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937185.1"
                     /translation="MKDLNNKVAVITGAGSGIGRALSVALANEGCALALVDISEKGLQ
                     HTLAILHGRHNKITTHVASVADRARMVALPAEIEQAHGAIHLLFNNAGVTINKSFEDS
                     SLEDLDFVININLYGVIYGCHFFLPYLKKQAESHIVNTSSLAGFL"
     gene            complement(455..1687)
                     /locus_tag="VFX11_00815"
     CDS             complement(455..1687)
                     /locus_tag="VFX11_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX4937186.1"
                     /translation="MNAHLEKCFAPAHLNGLELRNRFIKAGTYEGMTPGGIPSPALKE
                     FHTRIASGGTAMTTIGYCAVEADGRLNENMLYMHEGIRKQLRELISSVHAQGAKVSGQ
                     MGHGGGFSKNRELQRKRPLGPSFGINMLGAANGMFFCDAMTQADIDNLVRTYHDAAVF
                     MKSVGFDAVEIHFGHGYGLCQFMSPKTNRRKDEYGGSLTNRMRLPLRVLAAVRDAVGD
                     TFPVIGKISLTEGVRGGLDYDDAVEISMLLDKGGIDGIITSGGTSTMNPMIMFRGGNI
                     HKPLIRAEKNPLMKAIMYIVGPSLFKDYPYEELYFLEQAKRIREKVQCNMIYVGGAST
                     NASFERLMAEGFDFIQLGRTLLSDPFLPKQAARTEHYESRCIHCNECVATIEHPKGIH
                     CTRFGVSHTSTEKITNTI"
     gene            complement(1704..2426)
                     /locus_tag="VFX11_00820"
     CDS             complement(1704..2426)
                     /locus_tag="VFX11_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937187.1"
                     /translation="MTTTSNAPARTSALSRDRIIKAAYQIAKKDPNDALSMRKIATKL
                     KVTPMAIYKYFSDKSELIAAVVDHQMTESNLIPDNIDHSDWRSWVKESFLRMWDAYDS
                     SPGMAQYMSHATSFGPAVLNWQNETLKVLITAGLTPKQALTAHAAMAELATGSTILVP
                     IRQQGVEKVFPTIWKDLKAGKAPDLSELNNGPVSVVDYPWLLMCGQAMLENMHNSRDA
                     FSAELDLILDGLTLQLAENKKK"
     gene            complement(2445..3161)
                     /locus_tag="VFX11_00825"
     CDS             complement(2445..3161)
                     /locus_tag="VFX11_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX4937188.1"
                     /translation="MSVTRTSNILQAGTRAKSRRGRNPSIDLEKIEHAVREIGTQGAD
                     ISMKDVADFLGVNVTTLYRHVGGIDGLRRIRASLSRSDLEELPSPKGHTWQSWMRVLG
                     DYYRRALLQHPDLLDFVQAALDPDFVNLEKEASILVQFGFEPRAALMAHSFLITTVTG
                     YVQQELQSIEEARAGYAPYYARLFQNLDSQPERLPTLSSVNLSQKDLDRDLNFKAVVN
                     YAIAGIGAQRDAPKDADKKL"
     gene            complement(3327..>3802)
                     /locus_tag="VFX11_00830"
     CDS             complement(3327..>3802)
                     /locus_tag="VFX11_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006992390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4937189.1"
                     /translation="GYWGDPEKTAETIDAEHWLHSGDLGVMDEEGYVRIVGRIKDMII
                     RGGENVYPREVEEFLYTHPAIQEVQVFGIPDAKYGEEICAWVQLRSGASATAEDIRAY
                     CHDRITHFKIPRHIRFVDSFPMTVTGKIQKFKMREAMEAEQASGGQAPNKSVQPA"
BASE COUNT          660 a         1070 c         1160 g          912 t
ORIGIN      
        1 cagaaatcca gccaggctgg aggtgttgac gatgtgggat tccgcctgct ttttcagata
       61 gggcaggaag aaatggcagc catagatcac gccatacaga ttgatgttga tgacgaaatc
      121 caggtcttcc agcgagctgt cttcaaaact tttgttgatg gtgacgccgg cgttgttgaa
      181 cagcagatgg atggcgccgt gggcctgctc gatctctgcc ggcagtgcga ccatgcgtgc
      241 gcggtctgcc acgctggcta cgtgggtggt gatcttgttg tggcggccgt gcaagatcgc
      301 cagggtgtgc tgcaatcctt tttcggaaat atccaccagc gccagcgcgc agccttcgtt
      361 ggccagtgcc acggacaagg cgcgaccgat gccgctgcca gcgccggtga tcacggcgac
      421 tttgttattc aggtctttca tgttcgtact tccctcaaat cgtatttgtt attttttcgg
      481 tactggtgtg gctgacgcca aatcgggtgc aatggattcc cttggggtgt tcaatcgtgg
      541 cgacgcattc gttgcagtgg atgcagcggc tttcgtaatg ctcggtgcgg gccgcctgct
      601 tgggcagaaa cggatcgctc agcaaggtgc ggcccagctg gatgaaatca aatccttccg
      661 ccatcagccg ttcaaagctg gcgttggtgc tggcgccgcc gacatagatc atattgcact
      721 gcactttttc gcgaatgcgt ttggcctgct ccaggaaata cagttcctcg taggggtagt
      781 ccttgaacag cgatggtccg acgatataca tgatggcttt catcagcgga tttttttccg
      841 cccggatcag cggtttgtgt atgttgcctc cacgaaacat gatcatcgga ttcatggtgc
      901 tggtgccgcc gctggtgatg atgccgtcga tgccgccttt gtccagcagc atggagattt
      961 ccacagcgtc gtcataatcc aggccgccac gcacgccttc ggtcaggctg attttgccga
     1021 tcacgggaaa ggtatcgccc acggcgtcgc gcacggctgc cagaacgcgc agtggcagcc
     1081 gcatgcgatt ggtgagtgag ccgccgtact cgtccttgcg cctgttggtt ttcgggctca
     1141 tgaactggca caggccgtag ccgtggccga aatggatttc cactgcatcg aagccgacgg
     1201 atttcatgaa gacggcggcg tcgtgatagg tgcgcaccag gttgtcgata tcggcctggg
     1261 tcatggcgtc gcagaagaac atgccgttgg cggcccccag catgttgatg ccgaaactcg
     1321 gccccagcgg acgcttgcgc tgcagttccc ggtttttgga aaagccgccg ccgtgtccca
     1381 tctggccgga tactttcgcg ccctgtgcat gcacgctgct gatcagttcg cgcagttgct
     1441 tgcgaatgcc ttcgtgcatg tacagcatgt tttcattcag ccggccgtcc gcttccaccg
     1501 cgcagtagcc gatggtggtc atggcggtgc cgccgctggc gatgcgagtg tggaattcct
     1561 ttaatgccgg ggaaggaatc ccacccggtg tcatgccctc gtaagtgccg gccttgatga
     1621 agcggttgcg caattccagg ccgttcaggt gtgcaggggc aaaacatttt tccagatggg
     1681 cattcatgcg tgcgttcccg ccactatttc tttttatttt ccgccaattg caatgtcaaa
     1741 ccgtccagta tcaggtccag ttcggccgaa aaggcgtccc gactgttgtg catgttttcc
     1801 agcatggcct ggccgcacat cagcagccag ggataatcca ccaccgatac cgggccgttg
     1861 ttcagctccg acaggtcggg tgctttgccc gccttcaggt ctttccagat ggtgggaaat
     1921 actttttcca cgccctgctg gcggatcggc accaggatcg tgctgccggt ggccagttct
     1981 gccatcgcgg cgtgggctgt cagcgcctgt ttcggtgtca ggccggcggt gatcagtact
     2041 ttcagagttt cgttctgcca gttcagcacc gcagggccga agctggtggc gtggctcatg
     2101 tactgggcca tgcccggcga actgtcgtag gcgtcccaca tgcgcaggaa ggattccttg
     2161 acccagctgc gccagtcgct gtggtcgatg ttgtcgggaa tcaggttgct ttcggtcatc
     2221 tggtggtcga cgacggcggc aatcagttcg ctcttgtcgc tgaaatattt gtagatcgcc
     2281 atcggcgtca ctttcagctt ggttgcgatc ttgcgcatgc tgagagcgtc gttcggatct
     2341 ttcttcgcaa tctggtacgc ggccttgatg atgcggtcgc gggacagcgc actggtacgt
     2401 gcgggtgcat tcgatgtggt ggtcatggat acaacaattc cgcgtcagag ttttttgtca
     2461 gcatctttcg gcgcgtcgcg ctgggcgcca atgccggcaa tcgcataatt caccacagcc
     2521 ttgaagttca ggtcgcggtc gaggtctttc tgactcagat tgacgctgct cagggttggc
     2581 aaccgttcgg gctggctgtc cagattctgg aacaggcgcg cgtagtaggg cgcgtaaccg
     2641 gcccgggctt cctcgatcga ctgcagttcc tgctgtacat atccggtaac agttgtgatc
     2701 agaaaactgt gggccatcag tgcggcacgg ggctcgaaac cgaattgcac caggatgctg
     2761 gcttcctttt ccagattgac gaaatccggg tccagtgccg cctgcacgaa gtccagcaga
     2821 tcgggatgtt gcagcagggc gcggcggtag tagtccccca gcacgcgcat ccaggattgc
     2881 caggtgtggc ccttgggtga cggcagttcc tccaggtcgc tgcgcgacag cgaggcacgt
     2941 atccgccgca ggccatcgat tccgcccaca tggcgataca gggtggtgac attgacgccg
     3001 aggaaatcgg ctacgtcctt catggaaatg tcggcgccct gggtgccgat ctcgcgcacc
     3061 gcgtgctcga ttttttccag gtcgatggaa gggttgcggc cacgccggga tttggcccgg
     3121 gtgcccgcct gcaggatgtt gctggttctg gtcacgctca tgctggtttc cgttttgctg
     3181 tgatcgcgct gaattcaaac gccagaggcg aaaatgagga gggcaagtgc cgaaaatgcc
     3241 ctcccattgt aacgattcgc cgaccatttc gcacggtggt tgcaatactc attgcactat
     3301 cagataagaa gcaggccgta cgcgtgtcag gccggctgca ccgacttgtt cggtgcctgg
     3361 ccgccggacg cctgctccgc ttccatcgct tcgcgcattt tgaatttctg gatcttgccg
     3421 gtcactgtca tcgggaagct gtcgacaaag cgtatatgcc gcggaatctt gaagtgcgtg
     3481 atcctgtcgt ggcagtaggc gcggatgtcc tcggccgtgg cgctcgcgcc ggaccgcagt
     3541 tgcacccagg cgcagatttc ctcgccgtat ttggcgtccg gaatgccgaa cacctgcact
     3601 tcctggatgg cggggtgggt gtagaggaat tcctccacct cgcgcggata cacgttctcg
     3661 ccgccgcgga tgatcatgtc cttgatgcgg ccgacgatgc gcacgtagcc ttcctcgtcc
     3721 atcacgccca ggtcgccgga atgcagccag tgttctgcgt cgatggtttc agcggttttt
     3781 tccgggtcgc cccagtagcc ct
//
LOCUS       DASUEG010000050         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_638459, whole genome shotgun sequence.
ACCESSION   DASUEG010000050 DASUEG010000000
VERSION     DASUEG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638459"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFX11_00835"
     CDS             complement(<1..848)
                     /locus_tag="VFX11_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4937190.1"
                     /translation="MKRAKIPKCLIFFCLLLAGGHNGALAADSLNPWLYFLLDETQQS
                     PQQIAALPPSAWQSAGVQVPNLDYSAGVVWLRLDIPADRNLEPWLLEIRWPFYDSVEL
                     FELDDNTVTGPLAVAGDHHTMSGRHFASRFPLFELHGGGQPKTLLLRLRSGGLLLLPV
                     FQWQINDYIREEQKAQIVLGLFYGILLLIMLYNLGVWLLVRDHSHLFYAGYVFTVALY
                     QASLHGVGFQYLWQDSPFLADKMLSISVGGTYMFGALFFTFFLGLARRWPWLHRITWI
                     ISAVYLG"
     gene            1082..1276
                     /locus_tag="VFX11_00840"
     CDS             1082..1276
                     /locus_tag="VFX11_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HEX4937191.1"
                     /translation="MDVRQGEKNKTWFRSDRILHINDKWFFLTREQTQEGPFASRGEA
                     ERELTYYIRNMNHFSVIRNP"
     gene            1499..1999
                     /locus_tag="VFX11_00845"
     CDS             1499..1999
                     /locus_tag="VFX11_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937192.1"
                     /translation="MGISSQEFLQYVIQPTLRQLGVQSKGAEQLLLATAHHQSGLGKH
                     LQFRNGIGVYGISEQQHCEVWDHYLAFDSDLASQIRGMASQHEFPKAPHLELATNLSY
                     ATAIAWMIYRYHDACIPEVFSAQDLSKCWRECFLDRPASEKDAADFQCGYHDLMQTVA
                     ATSLAA"
     gene            complement(2031..>3028)
                     /locus_tag="VFX11_00850"
     CDS             complement(2031..>3028)
                     /locus_tag="VFX11_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX4937193.1"
                     /translation="AAAPSAPTTTTAPAKAAASVATAAAPAPASKVHAGPAVRLLARE
                     LGVDLGLVKSTGPRNRILKEDVHAFVKQRLSDKSVVTAAASGSGLPPLPEIDFSQFGP
                     VEMVELSRINKLSAANLHRAWVHIPHVTQFDEADITDLETFRKSESAALKDQDVKLTI
                     LAFMVKACAKSLLAFPRFNSSLHVGGEQLVFKKDVHSGIAVDTPTGLVVPVIRDADKK
                     SVLDIAKDMQVLSQKARAKKLSPGDMQGATFSISSLGGIGGTAFTPIVNWPEVAILGV
                     SKSQFKPVYNGKEFVPRLMLPLSLSYDHRVIDGAEAARFTAFLSQQLADIRRLLL"
BASE COUNT          736 a          858 c          809 g          625 t
ORIGIN      
        1 cccagataaa ccgcgctgat gatccatgtt atgcggtgca gccagggcca gcgccgcgcc
       61 agtccgagaa aaaaggtaaa aaacagcgcg ccgaacatgt aggttccacc aaccgagatc
      121 gacagcattt tatccgccag aaacggactg tcctgccaca gatactgaaa gcccacaccg
      181 tgcaatgacg cctgatacag cgccaccgtg aacacatagc cggcataaaa aagatgactg
      241 tgatcgcgca ccagcagcca gacaccaagg ttgtacagca tgatcagcag caggatgccg
      301 taaaagagcc ccagcacaat ctgcgctttc tgttcctcgc gaatgtaatc gttgatctgc
      361 cactggaaca ccggcaacag cagcaacccg ccgctgcgca ggcgcaacag caaggttttc
      421 ggctggccgc cgccgtgcaa ttcaaacagg ggaaagcgac tggcgaagtg ccggccggac
      481 atggtgtgat gatcgcccgc caccgccagc ggaccggtca cggtattgtc atccagttcg
      541 aacagttcaa cgctgtcgta aaacggccag cggatttcca gcagccaggg ttccagattg
      601 cggtcggctg gaatgtccag ccgcaaccac accacgccag cgctgtaatc cagattgggc
      661 acctgcaccc cggctgattg ccaggccgat ggcggcagcg ccgcgatctg ttgcggcgat
      721 tgttgcgttt cgtccagcaa aaaatacaac cacggattaa gtgaatccgc tgccagcgca
      781 ccgttgtgac cgccagccag cagcaaacag aaaaaaatca gacatttcgg aatttttgcc
      841 cgtttcacga aaacccgttg aattgaatag tgaaaaagtg gctcgtcatt caaggatagc
      901 aatgccggac gtggaacagc accggtttcg ctttattccg cttttcaggc agttacatcg
      961 ccatataccc aaaagcagca aaattgcact catctacatg aattgaatct tagatgcaga
     1021 tcacaaaact gtatattttt taatcaaccc ctggtagttc cactccactt cgaggtgatt
     1081 tatggatgtc aggcagggcg aaaaaaacaa gacatggttc cgcagcgatc gcattttgca
     1141 tatcaatgat aagtggtttt ttctaacccg ggagcaaaca caggaagggc cgtttgcttc
     1201 acgcggcgaa gccgaacgcg aattgaccta ttacatccgc aacatgaatc acttttcagt
     1261 tataagaaat ccctgacgtt gcgtggcaga caaactgtgt caacaaatgg caacggattg
     1321 ccatcccata tagcgaacac ttaccaaatg tcacgtcctg ccaacgctaa cggtattgaa
     1381 tttcccggct tccggaccga tcttaatagc gagtagcaga gatttcagtt tccaaggatc
     1441 atggcagact ctttgcttca acgtagtatg aagatgaagt tttgacggga gtgacgtcat
     1501 gggcatcagc agtcaggaat ttctgcagta tgtgattcaa cccaccctgc gccaattagg
     1561 cgtgcagtca aaaggtgcag aacagctttt gctggccacc gctcaccatc agtcgggtct
     1621 gggcaaacac ctgcagttca ggaacggcat cggtgtatat ggcatcagcg agcagcaaca
     1681 ctgtgaagtc tgggatcact atctggcgtt tgactccgat ctggccagcc agattcgcgg
     1741 catggcatca cagcatgaat ttcccaaagc accccacctg gaactggcca ctaacctgag
     1801 ttacgccact gcgattgcgt ggatgatcta ccgctatcac gatgcctgta ttcccgaggt
     1861 tttcagtgcc caggatctgt cgaagtgctg gcgcgaatgc tttctggatc ggccggccag
     1921 cgagaaggat gcagcggatt ttcagtgtgg ctatcacgat ctgatgcaga ctgttgctgc
     1981 caccagcctg gcggcgtgat ggcagcatta caggtcttga attgcaggaa ttacaaaagc
     2041 agtcggcgaa tatccgccag ctgctgtgac aggaaggcag taaagcgcgc agcctcggca
     2101 ccgtcgatca cccggtgatc atacgacagc gacagcggta acatcaggcg cggcacaaac
     2161 tccttgccgt tgtacaccgg cttgaactgg gatttcgaaa cgcccagaat cgccacctcc
     2221 ggccagttca caatcggcgt gaacgccgtg ccaccaatcc cgcccaatga cgaaatcgag
     2281 aacgtggcgc cttgcatgtc gcccggcgac agcttcttcg cccgcgcttt ctgactcagc
     2341 acctgcatgt ccttcgcaat atcgagcacg gattttttgt ccgcgtcgcg aatgacgggc
     2401 actaccagac cggtgggcgt atccaccgcg atgccgctgt gcacatcttt tttgaacacc
     2461 agctgttcac cacccacatg cagcgagctg ttgaagcggg gaaaggccag cagcgacttg
     2521 gcgcaggctt tcaccatgaa cgccagaatc gtcagcttca catcctgatc cttcagcgcc
     2581 gccgattcgg atttgcggaa ggtttccaga tcggtgatgt cggcttcgtc gaactgggtg
     2641 acatggggaa tgtgcaccca ggcgcggtgc aggttggcgg cgctaagctt gttgatacgg
     2701 ctcaactcca ccatttccac cgggccaaac tggctgaaat cgatttccgg cagcggcggc
     2761 aggccactgc cagatgcagc agcggtgact acggatttgt cactcaaccg ctgtttcacg
     2821 aaggcgtgca cgtcttcctt caatatgcga ttgcgtggcc cggtggattt taccaacccc
     2881 aggtccacac ccagttcccg cgccagcaga cgcacggcgg ggccggcatg gactttggaa
     2941 gcaggagcgg gggctgccgc cgttgcgaca gacgcagcgg ctttcgcagg cgctgtggtt
     3001 gtcgtgggag cggaaggcgc agcggctg
//
LOCUS       DASUEG010000051         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1853384, whole genome shotgun sequence.
ACCESSION   DASUEG010000051 DASUEG010000000
VERSION     DASUEG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853384"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFX11_00855"
     CDS             complement(<1..1141)
                     /locus_tag="VFX11_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4937194.1"
                     /translation="MDPIIIVGTGLAGYNLAKEYRKLNQARPLILLTADDGRNYSKPM
                     LSTGFTKQKSADELALADAATMAQQLNADVKTFVQVTAIDTAARTLTLSNHEQLTYGE
                     LVLAWGADVFRPPLAGDGVDQVYSVNDLLDYARFRAAVEGKKKVVIIGGGLIGCEFAN
                     DLANGGFSVEVVEPLGRPLPTLLPEPASRAVQAGLESLGVQFHFGPHVKAVNRNGERL
                     GVVLSDDTALDAEIVISAIGLRPRIKVAQDAGIAVNRGIRVDRHLRTSAANVYALGDC
                     SEVEGNVMLYVLPLMAGARALAKTLAGEATAVDYGPMPVAIKTPVCPVVVAPVPVGVA
                     GDWQIQADGANVNAQFIDATGVLRGFALTGDATGEKNKLAKLLPPL"
     gene            complement(1199..1363)
                     /locus_tag="VFX11_00860"
     CDS             complement(1199..1363)
                     /locus_tag="VFX11_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX4937195.1"
                     /translation="MRKYECIVCGYIYDEALGWPDDGIPAGTKWEDVPADWVCPDCGV
                     SKEDFELVQD"
     gene            1575..>2064
                     /locus_tag="VFX11_00865"
     CDS             1575..>2064
                     /locus_tag="VFX11_00865"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX4937196.1"
                     /translation="MTHPFLPSILHDPTLYRGGHGSVPPYRVPRTFRDWLLDPGSLTR
                     RLVRESGGHFRVQVLRQGFLPAYPEERRELGLEWREWPFVREVALRCHDTPWVFARTL
                     IPAATLKGPARALTHLGTKPLGAVLFNDPRVRRGPIAVSRLNGARLGRSWTAEGEIWG
                     RRS"
BASE COUNT          370 a          657 c          612 g          425 t
ORIGIN      
        1 gcaacggcgg caacagcttc gccagtttgt ttttctcgcc agtggcgtcg ccggtcaggg
       61 cgaaaccacg cagcacgccg gtagcgtcaa tgaattgcgc gttgacgttg gcgccgtcgg
      121 cctggatctg ccagtcgcct gccacgccca ccggcaccgg cgccaccacc accgggcaca
      181 ccggggtttt gatcgccacc ggcatcgggc cgtaatccac tgcggttgct tcacccgcca
      241 acgttttcgc cagcgcccgt gcccccgcca tcagcggcag tacatacagc atgacgttgc
      301 cttccacttc ggagcagtcg cccagcgcat acacattcgc cgcgctggtg cgcaggtggc
      361 ggtccaccct gatgccgcga ttcaccgcaa tgccggcgtc ctgcgccacc ttgatgcggg
      421 gacgcaaacc gatggcggaa atcacgatct ccgcgtccag cgctgtgtcg tccgacaaca
      481 ccacacccag acgttcgccg ttgcggttca ccgctttcac atgggggccg aaatggaatt
      541 gcacgcccag gctttccagc ccggcctgca cagcgcggga ggccggctcc ggcaacagcg
      601 tgggcagcgg acgacccagg ggttccacca cttcgacact gaaaccgccg ttggccagat
      661 cgttggcaaa ctcgcagccg atcagaccac caccgatgat gacgactttc tttttgcctt
      721 caacagcggc gcggaagcgg gcgtaatcca gcagatcgtt gacggaatac acctgatcga
      781 cgccgtcgcc ggccagtggc gggcggaaca catccgcgcc ccaggccagc accagttcgc
      841 cgtaggtcag ttgttcgtga ttggaaaggg tgagcgtgcg ggcagctgtg tcgattgccg
      901 tcacctgcac gaaggttttc acatcggcgt tgagttgttg tgccatggtc gcggcatccg
      961 ccagcgccag ttcgtcggca cttttttgtt tggtgaagcc ggtggacagc atgggttttg
     1021 aatagttgcg gccgtcatct gcggtgagca ggatcagggg gcgcgcctga ttcaattttc
     1081 ggtactcctt ggccaggttg tagccggcca atccggtacc cacgatgatg atggggtcca
     1141 ctgcgcggtc tccgtagtct ttgataaaga gcgaggtaat ggtgccgtgg tgagaggctt
     1201 agtcctgaac cagttcgaag tcttccttgc tcacgccgca atccgggcat acccagtcag
     1261 ccggcacatc ttcccatttg gttcccgcgg gaataccgtc atccggccat cccagtgctt
     1321 cgtcgtaaat gtagccacat acgatgcatt cgtactttct catgttggta ttaccccctg
     1381 cgtttacagc catttttgga cagtgcggat ttttgggaaa cggcaaatta cgtgccctcc
     1441 cctcaaaaag caagcgggaa aagtacaaag ccggcaccgt gacgcggaca gcaaaaggca
     1501 aacgtcgtca ttttggatag aatcagcatc cgtgctcccg aatggtttca atcatttagc
     1561 ttaagcaggt gtccgtgacc catccgtttc tgccttccat cctgcatgat cccaccctct
     1621 atcgcggtgg ccacggcagc gtgccgcctt atagggttcc ccgcaccttt cgcgactggc
     1681 tgctggaccc gggctctctc acccgccgcc tggtccggga gtccggcggc catttccgcg
     1741 tgcaggtatt acgacagggc tttctgcctg cctacccgga agaacgccgc gagctgggtc
     1801 tggaatggcg ggaatggccg ttcgtgcggg aagtggccct gcgctgtcat gacacaccct
     1861 gggtgtttgc gcgcaccctg atccctgccg ctacccttaa aggcccggcg cgggcactga
     1921 cccatctggg caccaagccg ctgggtgccg tactgttcaa cgatccccgc gtgcgacgcg
     1981 ggcccattgc ggtgtcgcgc ctgaacggtg cacggctggg gcggtcatgg acggcagaag
     2041 gtgaaatctg gggccggcgt tcct
//
LOCUS       DASUEG010000052         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_42022, whole genome shotgun sequence.
ACCESSION   DASUEG010000052 DASUEG010000000
VERSION     DASUEG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42022"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFX11_00870"
     CDS             complement(<1..175)
                     /locus_tag="VFX11_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX4937197.1"
                     /translation="MAEVVIVGRTRGGAEWVPEFVTALDAAKCIGCGRCYKVCPRDVF
                     ELVDRADVVEGDDV"
     gene            complement(208..414)
                     /locus_tag="VFX11_00875"
     CDS             complement(208..414)
                     /locus_tag="VFX11_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HEX4937198.1"
                     /translation="MSEDEVKALQKEVSQKKRIATEWASQIHDLVEDRLMTGYNDLPE
                     LARQTVAACEAWAAAKAKLDQVGA"
     gene            complement(429..905)
                     /locus_tag="VFX11_00880"
     CDS             complement(429..905)
                     /locus_tag="VFX11_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HEX4937199.1"
                     /translation="MSEAQLEVISEGAAALQHPFVKQMAVQLRAMDSYGTYDTWTDAR
                     VLDPLVLTKERRRQIPVVGDPDETTISRVKAYYNALAQTVERETGLLAVPVINLTHEG
                     FGRALILVGKLVALDKTLRDVHRFGFDSLEKMVEESEKLLSKAVGLINEHRTVAEL"
     gene            complement(994..1473)
                     /locus_tag="VFX11_00885"
     CDS             complement(994..1473)
                     /locus_tag="VFX11_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX4937200.1"
                     /translation="MTRITRQLRVLDDDDSDSTLLKVAFATSDGKVVDQHFGSAQGFA
                     IYGVNPERVQLLTLSEFGPLDQDGNEDKLVSKLALLQGCIAVYSRACGASALRQLLAI
                     EVQPVKVPEDALIEELLAALQAELREGPSSWLAKAIQRHRGVDPSRFDSMEAEGWND"
     gene            complement(1470..>2289)
                     /locus_tag="VFX11_00890"
     CDS             complement(1470..>2289)
                     /locus_tag="VFX11_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX4937201.1"
                     /translation="VSESIESFGLRPVLIPDLSGSLDGHLDDAQFNPLTTGGLSVKDL
                     AEVGESVATLMIGPSLTAAADLLHQRTAVPDFRFGHLQGLDEVDRWFATLAQISGRPV
                     PEKWIRHRRQLQDAMLDTHFMLGDVRAAIAADPDVLMGFEALLRGMGASTVAAVVPAK
                     NAALASVPVASVQVGDLEDVEKIAAQTKANLLFGNSHAGDSAKRLGIPLLRIGFPQYD
                     LVGGFQRCWFGYRGTQQALFDIANLMLAHHREIQPYYSIYAQKPAGETRTRHWT"
BASE COUNT          423 a          761 c          622 g          483 t
ORIGIN      
        1 ccacatcgtc gccctcgacg acatccgccc ggtccaccag ttcgaatacg tcacgcgggc
       61 acaccttgta gcaacggcca cagccaatac atttcgcggc atccagtgcg gtcacgaatt
      121 ccggcaccca ctccgcaccg ccccgcgtgc gccccacaat cactacttcc gccatggccc
      181 ctctcctctc tcgttcaagg caacctttta agcgccgacc tgatccagtt tcgccttcgc
      241 tgccgcccag gcttcgcagg ccgccacggt ctgccgcgcc agttccggca gatcgttgta
      301 gccggtcatc aggcggtctt ccaccagatc atgaatctgc gatgcccact cggtggcaat
      361 gcgttttttc tggctgactt ccttctgcag tgcttttact tcatcttcac tcatggatat
      421 ctctcgcttt acagttccgc cacggtgcgg tgttcgttga tcaggcccac cgccttgctc
      481 agcagctttt cggattcctc caccattttt tccagcgagt cgaagccgaa gcggtgcacg
      541 tcgcgcaggg ttttatccag cgccaccagt ttgcccacca ggatcagcgc gcggccgaaa
      601 ccttcgtggg tcaggttgat cacgggcacc gccagcaggc cggtttcgcg ctccaccgtc
      661 tgggccagag cgttgtaata ggctttgacg cgggaaatgg tggtttcgtc gggatcgccc
      721 actacgggga tctggcggcg acgttccttg gtgagtacca gcggatcgag cacccgcgca
      781 tcggtccagg tgtcgtaggt gccgtagctg tccatggcgc gcagttgcac cgccatctgt
      841 ttcacaaagg gatgttgcag cgcggcggcg ccttcggaaa tgacttctaa ctgtgcttcg
      901 ctcatggtgt cctcaccgaa ctgtagtgcc ctttcgagcg tgtattgatt caaaacgtgt
      961 attgattgtt gaaatatctt gcgtctgcgc gtgtcagtcg ttccagcctt ccgcttccat
     1021 gctgtcgaag cgcgacggat caacgcccct gtgacgctgg attgccttgg ccagccagct
     1081 ggacggacct tcgcgcaatt ccgcctgcaa tgccgccagc agttcctcga tcagcgcatc
     1141 ctccggcacc ttcaccggct gcacttcgat cgccagcagc tggcgcaggg ccgaggcgcc
     1201 acaggcgcgg ctgtacacgg cgatgcagcc ctgcagtaac gcgagcttgc tcaccagttt
     1261 gtcttcgttg ccgtcctgat ccagcgggcc gaattcactc agtgtcagca gctgcacccg
     1321 ctcgggattg acaccgtaaa tggcaaatcc ctgggccgaa ccaaaatgct gatccaccac
     1381 cttgccatca ctggtggcga aggccacctt cagcagcgtg gaatcgctgt cgtcatcatc
     1441 cagtacgcgc aattgccggg taattcgggt catgtccagt gcctcgtgcg cgtttcgccc
     1501 gcaggcttct gcgcatagat cgagtaatag ggctgaatct cgcggtgatg ggccagcatc
     1561 agattggcga tatcgaacag cgcctgctgg gtgccgcgat aaccaaacca gcaccgctga
     1621 aaaccgccca ccaggtcata ttgtggaaag ccgatccgca acaacggaat gcccaacctt
     1681 ttcgcactgt cgccggcgtg gctgttacca aatagcagat tggctttcgt ctgcgcggcg
     1741 attttttcca cgtcttccag atcacccacc tgcacactgg cgacgggcac gctggccagc
     1801 gccgcatttt tggcgggcac tactgccgcc accgtgctgg cacccatgcc gcgcagcaac
     1861 gcctcgaatc ccatcagcac atccggatcg gccgcgatgg cagcgcgcac atcccccagc
     1921 atgaagtggg tgtcgagcat ggcatcctgc aactggcgcc ggtgacgaat ccatttttcc
     1981 ggtaccggcc tgccgctgat ctgcgccagc gtggcgaacc agcggtccac ttcgtccagg
     2041 ccctgtaaat ggccgaaacg gaaatccggc acggcggtgc gctggtgcag cagatcggca
     2101 gcagctgtca ggctggggcc aatcatcagg gtggcaacac tctcgcccac ttccgccaga
     2161 tccttcaccg acagtccgcc cgtggtcagc ggattgaact gcgcgtcatc cagatgacca
     2221 tccagcgaac cggacagatc cggaatcagc accggacgca gaccgaaact ttcaatgctc
     2281 tcgctgaca
//
LOCUS       DASUEG010000053         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1831270, whole genome shotgun sequence.
ACCESSION   DASUEG010000053 DASUEG010000000
VERSION     DASUEG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831270"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFX11_00895"
     CDS             complement(<1..187)
                     /locus_tag="VFX11_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4937202.1"
                     /translation="MSLSPGAFGARRIVCLTEETTETLYLLGEQDRIVGISGFTVRPP
                     QARRDKPKVSAFTSAKID"
     gene            complement(311..1294)
                     /locus_tag="VFX11_00900"
     CDS             complement(311..1294)
                     /locus_tag="VFX11_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937203.1"
                     /translation="MPDFNRPHIIAVNGIELAVYEAMPATQVQRWPVILCHGMPELAY
                     SWRHQFAPLVAAGFHVIAPDIRGVGHSQVLPNKEDYTLQARLDDMCGLLDHYGYEKGI
                     FIGHDFGGTIVWGMGIYHQDRVAGLCACNSPFADMPMNPLDLYNMLYGPTNYFAYFQT
                     QACEDKFNEDPARTFRFYLRRDTGKGTNLSRSRAHDAESIGHVHWIHDDESTWPGEVL
                     LNDEELAHYADAYHKTGFGPALNWYRCLPLDYEHQKKVLPEGLPKITVPVLAVGSELD
                     FIAAYHFYDLLDGYCTDWEKTLIANAGHWTQQENPEALNQVLVEWLRRRFL"
     gene            1509..2909
                     /locus_tag="VFX11_00905"
     CDS             1509..2909
                     /locus_tag="VFX11_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937204.1"
                     /translation="MSFDFTGKVAIVTGAGGGLGFAYAKYLAEKGCNVVVNDLGGDTF
                     GYDGQPNSKIAEEAAKKINALGKGKAVPNGSDVSDYKGAEAMVAAAIKNWGRVDILIN
                     NAGITSPAVFPDVDRKEMDLHMGVHVMGAINTMRAAWPHMVKQKYGRIINTASDSLLG
                     FAPQIAYPTMKAALLGLTRNTGLLGEGYGITINAILPAAFTRMTSLLPPGDFRAHLER
                     DFQPDKLAPVVAYLCHEKCNITREIFSIGGGKFTRVVFAASEALPVDTTMESVETQMA
                     KLMTDDVSNLMLMKSSFDDLKNLGFSAEEYKMFQDMSATQGEHTPIVGVKIDSVDNVW
                     DITIKSPVGDQHSTLVLKSSGSAVKGHVINKEHGNQQVLEGRVENGDTLTWKCKLTKP
                     VPLTLTYTAQVDKDQRIGGKVVGTLMGKTVMDSKFSGTPVFGAKADQAKQESAQQASL
                     DAQKKPGILQRLFAKA"
BASE COUNT          743 a          972 c          945 g          623 t
ORIGIN      
        1 tatcgatctt cgcgctggtg aaagccgaga ctttcggctt gtcgcgccgc gcttgcgggg
       61 ggcgcacggt gaaaccggaa atgccgacaa tacggtcctg ctcgcccagc agatacaggg
      121 tttccgtagt ttcctcggtg aggcagacaa tgcggcgggc gccaaacgcg ccgggggaaa
      181 gggacatgga actccgctgc gacagcacag gcaattcggg aatgaatggc gcgagtgtag
      241 cgcagcggcg gacaacagac tattggcgat tcagcgctgg aaccggcaag ccggtttgta
      301 aaaataacct ttacaaaaac cgccgccgca accattccac cagcacctga ttcagtgcct
      361 cgggattttc ctgctgggtc cagtggcccg cgttggcgat cagggttttc tcccagtcgg
      421 tgcagtagcc atccagcaga tcataaaagt ggtaggcggc gatgaaatcc agctccgatc
      481 ccaccgccag caccggcacg gtgattttcg gcaagccttc cggcagcact tttttctgat
      541 gctcgtaatc cagcggcagg caccggtacc agttcagggc cggaccaaac cccgtcttgt
      601 gataggcgtc ggcgtaatgg gccagttctt catcattcaa cagcacctcg ccgggccagg
      661 tgctctcgtc atcgtgaatc cagtgcacgt ggccgatact ttcggcatcg tgcgcgcggc
      721 tgcgggacag gttggtgccc ttgcccgtat cgcgccgcaa ataaaaacgg aacgtgcgcg
      781 cgggatcttc gttgaatttg tcctcgcatg cctgcgtctg gaaataggcg aaataattgg
      841 tggggccata cagcatgttg tacaggtcga gcgggttcat cggcatatcg gcaaatggtg
      901 aattgcaggc acacaggccg gccacccggt cctggtgata aatccccatg ccccacacga
      961 tggtgccgcc aaaatcgtgg ccgatgaaaa tacctttctc gtagccgtag tgatccagca
     1021 ggccacacat gtcatccagc cgcgcctgca gggtgtaatc ctctttgttc ggtaacacct
     1081 gcgagtggcc gacgccccgt atatccggcg cgatcacgtg gaagcccgcc gccaccaggg
     1141 gcgcgaactg atggcgccag gaataggcca gctccggcat gccgtggcac aggatcaccg
     1201 gccagcgctg tacctgggtt gccggcatgg cttcgtacac cgccagctcg atgccattca
     1261 cggcaataat gtggggccgg ttgaaatcag gcatggcagt tctcccgctg cagtggtttt
     1321 tatcggactg attcatatgg gtttctggcg aggtattccg ggtaacgcat atccggcaca
     1381 gacaacgctg gcctttattg gtatacatcg tatactattg acccactgtt cacaatacag
     1441 attcaaattc accgggctgc acatgcaaca agccgaattg cactacaaca agaacggagg
     1501 gaaagctcat gtcatttgat ttcaccggga aagtcgccat tgtcacgggc gcagggggcg
     1561 gactgggctt cgcctatgca aaatatctgg ccgagaaagg ctgcaacgtc gtggtcaacg
     1621 atctgggcgg cgacaccttc ggctacgacg gccaacccaa ttccaaaatt gcggaagaag
     1681 ctgccaaaaa aattaacgcg ctcggcaaag gcaaagccgt tcccaacggc agtgacgtgt
     1741 ccgattacaa gggtgccgag gccatggtcg ccgccgcgat caagaactgg ggccgtgtgg
     1801 acatcctgat caacaacgcc ggcatcacca gccccgcggt atttcccgat gtggatcgca
     1861 aggaaatgga tctgcatatg ggcgtgcatg tgatgggcgc catcaatacc atgcgcgccg
     1921 cctggccgca catggtgaag cagaaatacg gccgcatcat caacactgca tccgattccc
     1981 tgctgggctt tgcaccgcag atcgcctacc ccaccatgaa agccgcgctg ctgggcctga
     2041 cccgcaacac cggcctgctg ggcgaaggct acggcatcac catcaacgcg attctgccgg
     2101 cagcctttac ccgtatgacc agcctgctgc cgcccggcga tttccgtgcc cacctggaac
     2161 gggatttcca gccggacaaa ctggcgccgg tggtggctta tctgtgccac gaaaaatgca
     2221 acatcacccg cgagattttc tcgatcggcg gcggcaagtt cacccgcgtg gtgttcgcag
     2281 ccagcgaggc cctgccggtg gacacgacca tggaatccgt tgaaacgcag atggccaagc
     2341 tgatgaccga cgatgtgtcg aacctgatgc tgatgaaatc gtccttcgac gatctgaaaa
     2401 atctgggctt ctctgcggaa gaatacaaga tgttccagga catgtcggcc acccagggcg
     2461 agcacacgcc aatcgtgggc gtgaaaatcg acagcgtgga caatgtgtgg gacatcacta
     2521 tcaagtcacc ggtgggcgac cagcattcca ccctggtgct gaaatccagc ggcagcgcgg
     2581 tgaaaggtca cgtgatcaac aaggaacacg gcaaccagca ggtgctggaa ggtcgcgtgg
     2641 aaaacggcga caccctgacc tggaaatgca agctgaccaa gccggtgccg ctgacgctga
     2701 cctacaccgc gcaggtggac aaggaccagc gcatcggcgg caaggtggtc ggcacgctga
     2761 tgggcaagac cgtgatggat tccaaatttt ccggtacgcc ggtatttggt gccaaagccg
     2821 atcaggccaa acaggaaagc gcgcaacaag cttcattgga tgcacagaag aaacccggca
     2881 ttctgcagcg gctgtttgcg aaggcctgac aacaggcgcg ggccagtaca actggcctgc
     2941 gaattccggt agcagaagcc ccgcgtgtcg cggggctttt catttcaggg cgccggtttg
     3001 ccctgctccc tcatcaccac tatcatttgc attaccctcc caagacagcc aacctcatca
     3061 ggacaagccc gccgtctgcc tatgagcctg tatctgatcg tcaaagccca tgccacggaa
     3121 cagcagccat gactgaacat cagaacggca tctggttcat ctacgacggc gagtgtccaa
     3181 tctgccagta cgccgcccac gcgctgcgca tcaaatggca atttggcgaa ctgcatctgc
     3241 tggacgcccg cacgcagcag gatcatccgc aggtgcgcgc tgt
//
LOCUS       DASUEG010000054         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1742799, whole genome shotgun sequence.
ACCESSION   DASUEG010000054 DASUEG010000000
VERSION     DASUEG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742799"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..590
                     /gene="tnpA"
                     /locus_tag="VFX11_00910"
     CDS             159..590
                     /gene="tnpA"
                     /locus_tag="VFX11_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX4937205.1"
                     /translation="MDEFDSLSHSKWECKYHVVFIPKCRRKTLYGQLRPHLGEVFRKL
                     AKQKESTIEEGHLMSDHVHMLISIPPKYAVSQVVGFIKGKSAIHLARVYGEKRRNFVG
                     QHFWARGYFVSTVGRDEEMIREYIRNQEHEDQRLEQMNLWR"
     gene            745..>3010
                     /locus_tag="VFX11_00915"
     CDS             745..>3010
                     /locus_tag="VFX11_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4937206.1"
                     /translation="MSTSELPPPVTSDPVSDRAFRTVLARQVVVPLGFGLVTIVFFVV
                     IIYYLLSLSRWMESTEEIVSDSNLLMQLLVDHETAVRGFVITGEEEFLEPFRLAERRV
                     KPIIEKIQTSLGKDERIQRGRLDSVYESYEEWLQYANRLADAKRQNLKSFEMVNSREG
                     KRLMDQMRLLIAEFVAQAKLVKAEHRSQANLRVVVLSVTYFLISLLFSGLMAYGGRRQ
                     LRDLSYTYARLMHRQQEQTRALEEEAWLHAGESGVADCIIAQPSTLALAQSVLTFLAN
                     YLGGRVGAVYLADNGDGFSRIAEFGFSRTMATQEQTLAGNEGLVGQAASERALLYIDD
                     IQSSYFKVNSGLGEADPAALVLLPLLEENRVIAVLELGFLRALDPKERELLDRVSDNL
                     GTAFAAALYRQRLREMLLNVQQLNDELQSQQEELRVANEELEGQSQLLEESHARLETQ
                     RAELEESNLALMHQRQALRNKNDTLIQTQQLLEEHARELERASRHKSEFIASMSHELR
                     TPLNSQLILSQLLAENAQGNLTPDQVRNAKAVYAAGKDLLALINDVLDIAKIEVGKLE
                     VRPSQTRLADLLESLTAGFSAMAQEKNLNFQIHVAPDVVPELFTDRQRLEQILRNLLT
                     NAIKFTERGHVRLDVTVAEDGRVRFAVVDTGIGISADAMDYIFDPFRQVDSGNARKYG
                     GTGLGLSISRQLASLLGGSIHATSVPGAGSTFILDIPVTLTVNPPSEELDTVEAAIAV
                     PPPALMESPARHVED"
BASE COUNT          680 a          771 c          917 g          642 t
ORIGIN      
        1 cgccatttgg ccccgttatg gggtaatgcc agtcagttaa cccataaagg gaaatccccc
       61 ggctccgccg gggagacagc aaacgtttga catatcctgg agtccatcgc ggaaactccg
      121 aagcgtgagc cgccaagcac acgtaacgga gaatcgcgat ggacgaattt gacagcctaa
      181 gtcactccaa atgggagtgc aaatatcatg tagtgtttat tcccaagtgc cgacgaaaga
      241 cgttgtacgg tcagctcagg ccgcatttgg gtgaggtatt tcgcaagctg gcgaagcaga
      301 aagaaagcac gatagaagaa ggccatctga tgtcggatca cgtgcacatg ctgatatcga
      361 tcccaccgaa atatgctgtt tctcaggtgg ttggatttat caagggcaag agtgcaatcc
      421 acttggccag ggtttacggc gagaagcgcc gtaacttcgt aggtcagcac ttctgggcgc
      481 gaggctattt cgtttccacc gtgggtcgag acgaagaaat gattcgggag tacattcgga
      541 atcaggagca tgaggatcag cgcctggaac agatgaatct atggcgctga gaaccaccga
      601 atggtggttg ctataaatgc ggggccgcgt tagcgacccc gaatcagccg cttcgagcgg
      661 ctcacacaat taaagccccc ggctttgccg ggggatattt actcccttcg gccgtagctg
      721 gccggataag agcatcgata gccgatgagt acatccgaac tgccacctcc tgttaccagc
      781 gatcctgtca gtgatcgagc attccgtacc gttcttgccc gacaagtggt ggtgccactg
      841 ggatttggtc tggtcaccat tgtttttttt gtggtgatta tctattacct gctgtcattg
      901 agccgatgga tggaaagcac ggaggaaata gtcagtgatt ccaatcttct gatgcagctg
      961 ctggtggatc atgaaacagc cgtgcgcgga tttgtcataa cgggtgagga agagtttctc
     1021 gaaccctttc gcttggccga acggcgggta aaaccgatca ttgaaaagat ccagacgagt
     1081 ctgggtaagg atgagcggat tcagcgcggc agacttgata gcgtctacga aagctatgaa
     1141 gaatggctgc agtatgccaa tcggctggcg gacgcaaaac gtcagaatct gaagtccttc
     1201 gaaatggtga attcacgcga gggcaaaaga ttgatggacc agatgcgttt gctgatcgcc
     1261 gaatttgttg cccaggcaaa actggtcaag gccgaacacc gcagccaggc gaatctgcgg
     1321 gtggtcgtgc tgtccgtcac ctacttcctg atcagtctgc tgttcagtgg attgatggcc
     1381 tatggtggtc ggcggcagtt gcgggatctc tcgtacacct atgcccgcct gatgcaccgg
     1441 cagcaggagc agaccagggc gcttgaggag gaagcctggc ttcatgcagg ggagagtggc
     1501 gtggcggact gcattatcgc ccagccttca acgctggccc tggcgcagag cgtgctcact
     1561 tttctggcga attatcttgg tggcagggtc ggtgccgttt atctggccga taatggcgac
     1621 ggcttttccc gtatcgcgga atttggcttt tcccgaacca tggccaccca ggagcagacg
     1681 ctggcaggga atgaagggct ggtgggacag gcggccagtg agcgcgctct gctgtatatc
     1741 gatgacatcc agtccagcta tttcaaagtg aactccggtt tgggggaggc cgaccctgca
     1801 gcgctggtct tgttgccgct gctggaggag aaccgggtta ttgcagttct ggagttgggg
     1861 ttcctgcgtg cactggatcc gaaggagcgt gagctgctgg accgtgtgag tgataatctg
     1921 ggaactgcgt ttgcggctgc cctttatcgt cagcgcctgc gcgaaatgct gctcaatgtc
     1981 cagcagctga atgacgagct gcagtcccag caggaagagc tgcgcgttgc caacgaagaa
     2041 ctggaggggc aatcgcagct gctggaggag tcccacgccc gtctggaaac gcagagggct
     2101 gaactcgaag agagcaatct ggcactgatg catcaacgtc aggctctgcg caacaagaat
     2161 gataccctga ttcagacgca gcagcttctg gaggaacatg cccgtgaact ggagcgggcc
     2221 agccgccaca aatcggaatt cattgccagc atgtcccacg agctgcgcac tccgctcaac
     2281 agtcagctta tcctctccca gctgctggca gaaaatgcgc agggcaatct gacgcccgat
     2341 caggtgcgca acgcgaaagc ggtctacgcg gccggcaagg atctgctggc cctgatcaat
     2401 gacgtgcttg atattgcaaa aattgaggta ggaaaactgg aagtacggcc cagccagacc
     2461 aggctggcgg atttgctcga gtcattgaca gccggcttca gcgcgatggc gcaggaaaag
     2521 aacctcaact tccagattca tgtagcgccc gatgtcgtgc cagagctgtt taccgatcgc
     2581 cagcggctgg agcagattct gcggaacctg ctgaccaatg ccattaagtt cacagagcga
     2641 ggtcatgtcc gtctggacgt aacggtcgct gaagatggac gagtccgatt tgcggtcgtt
     2701 gataccggta tcggcatcag tgcggacgcc atggactata tctttgatcc ttttcgccag
     2761 gtggacagtg gcaacgcccg caaatacggt ggcacaggcc tggggctgtc catttcgcgc
     2821 cagctggcga gcttgctggg tggcagcatc cacgcaacca gtgtgccggg cgcgggaagc
     2881 acgtttatcc tggatattcc ggtgacgctg acagtgaatc cgccgtcgga agaactggac
     2941 actgtggaag ctgcgatcgc tgttccaccg ccggcgctga tggagtcacc ggcccggcat
     3001 gtggaagacg
//
LOCUS       DASUEG010000055         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1499260, whole genome shotgun sequence.
ACCESSION   DASUEG010000055 DASUEG010000000
VERSION     DASUEG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499260"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VFX11_00920"
     CDS             complement(<1..1541)
                     /locus_tag="VFX11_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4937207.1"
                     /translation="MPLQFTPHDQQLLLAAFEHAAIGAAIAADDGRWLRVSQALCDML
                     GYTAAELLATDCQSITHPDDVENDRHHLDLLRQGELPNCMLEKRYLHRNGHPVWVRFN
                     AFYARAGEQNKPHFVLLVQDISADREHLLQIRRNARRLHEIVTTQAMLANAQLDLAAF
                     MQTVVERMQAITPASGVVVELIEGDEMVYRAGSGTGAAFVGKRLKINGSLSGLCVQQR
                     TLLQSDDTASDPRVDAEACRLVGAGSMIVAPLIKDGDAAGVLKVLALQPHAFSADDAQ
                     TLQLMAGLMGSALAHQMAFDTNQTLLRERSRAIEALQSEMDLRNRYENFLRASEARTR
                     RIIEGSLDAFVGMNHAGIITEWNPQAEKTFGWLRDEVIGKPMAGILIPSAMRDAHFGG
                     LIRYNQTGVPVMLDRRIQAMALTRERGEIPVEMTISAIRIEGQYQFHAFLHDITERKK
                     NEARLQQMAQHDQLTGLPNRNLFFDRLEQALARNVRNRDWLALMYLDIDHFKQVNDSL
                     GHAAG"
     gene            complement(1651..3510)
                     /locus_tag="VFX11_00925"
     CDS             complement(1651..3510)
                     /locus_tag="VFX11_00925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937208.1"
                     /translation="MASSFRWILFACCWLLTFSLPGRADTVLHLSASDRFYNPAAHAA
                     YLIDDASTLTLDALPTDPAAWQPVPADSLNLGYLTGAVWLKWQFQRSADMPPVWYLLL
                     QYPMLDQIDVYLRPAGTRAEPMHVALGDRLPFAQRPLRHPDFVVPLNLDSGVVYDLFI
                     RVLSKGSLQVPVSMVAPEHFLQEDRIRTLLEGGSYFICLIMALYNFMLFLFLRDKSYF
                     FYVSYILMFVVALGANRGWGFEFLWPESPRVQEWAIVLGTLFAIASACAFATSFMRLS
                     QLAPLLHRIISGLAIACVALALVSVNLDYRLAIKLMVGFSLVCSLSLALMAFGFWYRT
                     RSRQAALYCISWLFFLAGVSLYMGNKLGLLPVNFLTEQGLRVGALVEIVFLSFALADR
                     VNQDRAASEAAQKKVIEMQLSMNVELEKQVKSRTQELEYLNHLLLQASITDALTATAN
                     RRHFDDMLDKEYRRAAREGTPLALLMVDLDYFKKINDTHGHLVGDMCLQNAARALTAC
                     IKRPPDMVARYGGEEFAVLLPNTPLEGARTVAESIRRKIESLTISIPSGQQLRLTVSV
                     GAAAHQPTQRDGHTQLVQEADTALYQAKHAGRNRVMPADTSTAPPSSAGDTAP"
     gene            complement(3644..4282)
                     /locus_tag="VFX11_00930"
     CDS             complement(3644..4282)
                     /locus_tag="VFX11_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4937209.1"
                     /translation="MRTPDPLQPAAGWHHIPQPRLTPPPPSQRGLFFRSLALVARRLG
                     RAEIPDIFPVLNIHRGLFPAWLWFASRLMPYGKLPAPVRELLILRTAWNCRCRYEWGQ
                     HVDLALVAGVSDADILASTRGAAGFADRHYAVLMQACDELCQQDAVSSDVWQALQKNW
                     DEASLIEIMLLVGNYRMLAGFLNSSGLVLEAPIEQRVQAFLQRVADDIPAAG"
     gene            complement(4269..5363)
                     /locus_tag="VFX11_00935"
     CDS             complement(4269..5363)
                     /locus_tag="VFX11_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937210.1"
                     /translation="MDQNPSSQPLHEATVEQTQAWISMHHVQHMVRSGRAAGINIDRL
                     LALAGLDDERLADGDATVPLRTIELLLAALQQDHADTLPGLHLANHIQPATLGALGFI
                     LQACTSFADLLDVVQRYNGMLSNIGHSSVLHQPGLVQLQWECRSGSAALRRHASEYVI
                     GTFVVVARMLAPAHEWAPVAVHFAHARPANPDRVREYFAFFRCPVFFGQPVSGISFPA
                     SLLPLRLPHGDAVLKDILERHGQNLLQQRTRTPSLVEDVRRLLSALMLQGVPSKESVA
                     QQLGTSSRSLHRRLQEQGTGYRDILDAVRLEQARALLLDSDATSADIADRLGFRSRQA
                     FIRWFSQHTRLTPGEFRRTRKTGDCPDADT"
BASE COUNT         1050 a         1644 c         1770 g          996 t
ORIGIN      
        1 tcgccagcgg cgtggcccag gctgtcgttc acctgcttga agtgatcgat gtccaggtac
       61 atcaatgcca gccagtccct gttgcggaca ttgcgggcca gtgcctgttc cagccggtcg
      121 aaaaacaggt tgcggttggg caggccggtg agctggtcgt gctgtgccat ctgctgcaga
      181 cgtgcttcat ttttcttgcg ctcggtgatg tcgtgcagga aagcgtggaa ttggtactgg
      241 ccttcgatcc tgatggcgct gatagtcatt tccaccggga tttcaccgcg ctcacgggtg
      301 agcgccatgg cctggatgcg gcgatcgagc atgaccggca caccggtctg gttgtagcgg
      361 atcagtccgc caaaatgggc gtcgcgcatg gcggacggaa tcagtatgcc ggccatgggt
      421 ttgccgatca cttcatcacg cagccagccg aaggtttttt ccgcctgtgg attccattcg
      481 gtgatgatgc cagcgtggtt catgccgacg aaggcatcga gcgatccttc gatgatgcgg
      541 cgggtacggg cctcgctggc gcgcaggaaa ttttcatagc ggttgcgcag atccatttcg
      601 gactgcagcg cctcgatcgc gcggctgcgt tcccgcaaca gggtctgatt ggtgtcgaac
      661 gccatctggt gggccagcgc gctgcccatc aatccggcca tcagctgcag ggtctgcgca
      721 tcgtcggcgc tgaaggcgtg aggctgcagt gccagcactt tcagcacccc tgcggcatca
      781 ccgtccttga tcaggggcgc cacgatcatc gaaccggcgc ccaccaggcg gcaggcctcg
      841 gcatccacgc gcggatcgct ggcggtatcg tccgattgca gcagggtgcg ctgttgtacg
      901 cacaggcccg acaggctgcc gttgattttg agccgcttgc ccacgaatgc cgctcctgtg
      961 ccactgccgg cgcggtagac catctcgtcg ccttcgatca gttccaccac cacaccactg
     1021 gcgggcgtga tggcctgcat gcgttccacc accgtctgca tgaacgcagc cagatccagc
     1081 tgcgcgttcg ccagcatcgc ctgggtggtg acgatctcat gcagacggcg ggcgttgcgg
     1141 cggatctgca gcaggtgttc acggtcggca gaaatgtcct gcaccagcaa gacgaagtgc
     1201 ggtttgttct gctcaccggc tcgggcgtag aacgcattga agcgcaccca gacaggatga
     1261 ccgttgcgat gaagatagcg tttttccagc atgcagttgg gcaattcgcc ctgtcgtagc
     1321 aggtcaaggt ggtggcggtc gttttcgacg tcgtcaggat gggtaatgga ttggcaatcg
     1381 gtcgccagca gttctgcagc ggtgtatccc agcatgtcac agagggcctg gctcactctc
     1441 agccagcgac cgtcatccgc ggcaatggct gcgccaatgg cggcatgttc gaatgcggca
     1501 agcagcaatt gctggtcgtg gggggtgaat tgcagtggca tgaccgacaa cacctgaaaa
     1561 tcccttgtca attgagtgta acggctgggc ccgatcacgc aaattgtagg gatttaatgc
     1621 acgaactgtg agggtataag cgcagtggtg tcagggggcg gtgtcgcccg cagatgatgg
     1681 tggtgcagtg gaggtgtcag cgggcatcac ccggttgcgg ccggcgtgct tggcctggta
     1741 tagcgccgta tccgcctcct gcaccagttg tgtgtggcca tcgcgctgcg ttggctggtg
     1801 cgcggcggca ccgacactga cagtgagacg caactgctgg ccggagggaa tgctgatggt
     1861 cagcgattcg atcttgcgcc ggatactttc cgccaccgtg cgggcgccct ccagcggggt
     1921 gttgggcagc agcacggcaa actcctcgcc accgtagcgg gctaccatgt ccggtggccg
     1981 cttgatgcat gcggtcagcg cgcgcgctgc attctgcaga cacatgtcgc ccaccagatg
     2041 gccatgggtg tcgtttattt tcttgaaata gtccagatcc accatcagca gggccagtgg
     2101 tgtgccttcg cgggcggcgc gccggtattc cttgtcgagc atgtcgtcga agtggcgtcg
     2161 gttggcggtg gcggtgagcg cgtcggtgat gctggcctgc agcagcagat gattgaggta
     2221 ttccagttcc tgggtacgcg atttcacctg tttttccagt tccacattca tcgacagctg
     2281 catctcgatc actttctttt gcgctgcctc ggaggcggcg cgatcctggt tcacccggtc
     2341 tgccagggca aatgacagga acacgatctc caccagcgcg cccacgcgca ggccttgttc
     2401 ggtgagaaaa ttcaccggca gcaaacccag cttgttgccc atgtacaggc tcacgcccgc
     2461 cagaaaaaac agccacgaaa tgcagtacag cgccgcctgc cgcgatcggg tgcggtacca
     2521 gaagccgaag gccatcaacg ccagtgacag cgaacagacc agggaaaatc ccaccatgag
     2581 tttgatcgcc aggcggtaat ccagattcac cgacaccagg gccagcgcca cacacgcaat
     2641 ggccagaccg ctgatgatcc ggtgcagcag gggcgccagc tggctcaggc gcatgaacga
     2701 cgtcgcaaac gcgcaggcac tggcgatggc gaagagtgtg cccagcacga tggcccattc
     2761 ctgcacccgc ggtgattccg gccacaggaa ttcaaagccc cagccacgat tggcacccag
     2821 ggccaccacg aacatcagga tgtacgacac gtagaagaag taactcttgt cccgcaaaaa
     2881 caggaacagc atgaagttgt atagcgccat gatcaggcag atgaagtagg agccgccttc
     2941 cagcagcgtt ctgatgcgat cctcctgcag gaaatgctcc ggcgccacca tgctcaccgg
     3001 cacctgcagt gagcccttgc tgagcacccg gatgaacagg tcgtagacca cgccggagtc
     3061 caggttcagc ggcacgacaa aatccggatg acgcaggggc cgctgcgcaa agggcaggcg
     3121 gtcgcccagc gcaacatgca tcggttcggc gcgggtgccg gccgggcgca ggtacacatc
     3181 gatctgatcc agcatcggat attgcagcag caggtaccag accggcggca tgtcggcgct
     3241 gcgctggaat tgccatttca gccacacggc accggtgagg tatcccaggt tcaggctgtc
     3301 ggccggcacc ggttgccagg cggccgggtc ggttggcagt gcatccagtg tgagggtgct
     3361 ggcgtcatcg atcaggtagg ctgcgtgggc agcgggattg taaaaccggt cgctggcaga
     3421 caggtgcagc acggtatcgg cccggcctgg caggctgaat gtcagcagcc agcaacaggc
     3481 aaacagaatc caccggaaag aagaggccat gggcgggaac gcactccata cgctgaaaaa
     3541 ccagaaccgc tacagcaagt atagaaaagg cccgccaaaa tcctgctctg ccgcgcgggg
     3601 aaaaataatg gccggtaaaa caaggaaatg cggctgcgca ccttcagccc gcggcgggaa
     3661 tgtcgtccgc cacgcgctgc aggaaggcct gcacccgctg ctcgatgggc gcttccagca
     3721 ccagtccact gctgttgaga aaacccgcca gcatgcggta gttccccacc agcaacatga
     3781 tttcgatcag cgacgcttcg tcccagtttt tttgcagtgc ctgccacacg tcgctgctga
     3841 ccgcatcctg ctggcagagc tcgtcgcacg cctgcatcag cacggcataa tggcggtcgg
     3901 caaaaccggc cgcgccgcgc gtggaggcca gaatgtccgc gtcactcacc ccggccacca
     3961 gcgccagatc cacatgctgt ccccactcat agcggcagcg acaattccat gcggtgcgca
     4021 ggatcagcag ttcgcgcacc ggcgccggca gttttccata cggcatcagg cgcgatgcaa
     4081 accacagcca cgccggaaac agaccgcgat gaatattcag cacggggaaa atatcgggga
     4141 tttccgcgcg tcccagacgc cgggccacca gcgccaggct gcgaaagaaa agtccgcgct
     4201 gcgacggcgg tggcggggtc aaccggggtt ggggaatgtg gtgccatcct gcggcgggct
     4261 gcaagggatc aggtgtccgc atcggggcaa tctcctgttt ttcgggtacg gcgaaattcg
     4321 cctggggtta accgggtgtg ctggctgaac cagcggatga aggcctgacg ggagcggaat
     4381 cccagacggt cggcgatgtc ggcgctggta gcgtcgctgt ccagcaacag ggcgcgggcc
     4441 tgttccagcc gcaccgcgtc cagaatgtcc cggtaaccgg taccctgttc ctgcaggcgc
     4501 cggtgcagac tgcggctgct ggtgcccagc tgctgtgcca cggattcctt cgagggaacg
     4561 ccctgcagca tcagggccga cagcaggcgc cgcacatcct ccaccagcga gggcgtccgg
     4621 gtgcgttgct gcagcagatt ctggccgtgg cgttccagaa tgtccttgag caccgcatcg
     4681 ccgtggggca ggcgcagtgg cagcaggctg gcagggaaac tgatgccgga caccggctgc
     4741 ccgaagaaca ccgggcagcg gaaaaaggca aagtattccc gcacccgatc cggattggcg
     4801 gggcgggcat gggcgaagtg caccgcgacc ggtgcccatt catgcgccgg tgccagcatc
     4861 cgcgccacca ccacgaatgt gccgatcaca tactcgctgg cgtggcggcg taacgccgcg
     4921 ctgccactgc ggcattccca ttgcagttgc accaggccgg gctggtgcag caccgaggaa
     4981 tggccgatgt tgctgagcat gccgttgtat cgctgcacca cgtcgagcag atcggcaaag
     5041 gaggtgcagg cctgcaggat gaagccgagg gcgcccaggg tggcgggctg gatgtggttg
     5101 gccagatgca gccccggcag ggtgtcggca tgatcctgtt gcagcgcggc cagcagcagt
     5161 tcgatggtgc gcaggggcac ggtggcgtcg ccgtcggcca ggcgctcgtc atccagtccc
     5221 gccagtgcca gcagccggtc gatattgatg cccgccgccc ggccgctgcg gaccatgtgc
     5281 tgcacatggt gcatggaaat ccaggcctgc gtctgttcaa cagtcgcttc atgcagaggc
     5341 tgggaagacg ggttctgatc cacgggaacg gttcctcgat gcagcgcgga tgacgcagtg
     5401 ccggtgtgaa gccatggtga cacaatttcg gctatgacgg gcatgctggc cggttttggc
//
LOCUS       DASUEG010000056         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1079710, whole genome shotgun sequence.
ACCESSION   DASUEG010000056 DASUEG010000000
VERSION     DASUEG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079710"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..2717
                     /gene="uvrD"
                     /locus_tag="VFX11_00940"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             504..2717
                     /gene="uvrD"
                     /locus_tag="VFX11_00940"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEX4937211.1"
                     /translation="MSADPIVNSLNEAQRRAVTSDADRLLVLAGAGSGKTRVLVHRIA
                     WLIQARHLAPHNILAVTFTNKAAAEMRHRIESLLRAPLGAMWVGTFHGIAHRFLRLHW
                     QEAKLPQNFQILDSDDQLRLVKRVMRGMELDETRWPPKQAQGFINANKDEGLRPEHID
                     DYGDIHQKTLVKIYQQYQDACETGGMVDFAELLLRSHELWLKHPALLQHYRQRFRHVL
                     VDEFQDTNRIQYAWLRVLVGNDNAITIVGDDDQSIYGWRGARVENIRQFGDDFGRAEV
                     VRLEQNYRSTSIILNAANGVIAHNSDRLGKNLWTSGAEGDPISVYAGFNEVDEARFIA
                     ERIQQALQLGHRRDDIAILYRSNAQSRVLEEALLRAAIPYRIYGGHRFFERAEIKNAL
                     AYLRLICHRDDDAAFERVVNTPTRGLGDKSLERIRALARELKIPLWQASLQVLQHREL
                     ATKAGNALKGFLALIEQLDQETRGTGLSEQAERVIEASGLIAFHQNEKDDRGEARVEN
                     LKELISATREFEISLQQESATLAAFLDHAALEAGETQAGENEDSVQMMTLHAAKGLEF
                     PIVFMAGVEEGLFPSMQSADDPHRMEEERRLCYVGITRAMKNLYITYAESRRIYGSEA
                     LHAPSRFIREIPLHLLQEVRASARLTRAMSTFDSGFGGVRERSAGYRSNTYGGARIQA
                     DDSPYQLGRSVRHPKFGDGVIMGCEGQGPNARVRVNFRGEGEKWLVAQYARLEFV"
     gene            complement(2778..3182)
                     /locus_tag="VFX11_00945"
     CDS             complement(2778..3182)
                     /locus_tag="VFX11_00945"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4937212.1"
                     /translation="MDEQQIDDIPTPVGELALQTIAMPADANADGDIFGGWLVSQMDL
                     AGAVTANRLARGRVATVAIDSMVFLRPVAVGSVVSCHTEVLNIGRSSITIRVEVWAQH
                     HSDTECRKVTEGQFTFVAIDENRRTRVVPRTA"
     gene            3587..>4160
                     /locus_tag="VFX11_00950"
     CDS             3587..>4160
                     /locus_tag="VFX11_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4937213.1"
                     /translation="MNFKKMIAAVSLTAGAAMLVSAAHAATNVDPKLPAYAKVGGISG
                     NLSSVGSDTLANLMTFWAEDFKKAYPNVAVQIQAAGSSTAPPALTEGTASFGPMSRPM
                     KDQEIEAFEKKFGYKPTAVPVAIDALAVFVHKDNPITGMTMEQVDAVFSNTRTCGASK
                     DVSKWGDLGLTGAWAARDLQMYGRNSVSGTY"
BASE COUNT          849 a         1195 c         1270 g          846 t
ORIGIN      
        1 cgctggcgcg ccaacctgcg ccagccgaca cagtcgtatc catttgaacc ttgaaatcac
       61 cgctttcata gtccaacgca taagccggag tcactgcgcc ggccgctgca attgctgcag
      121 tcagggcggt gaccttgaag gggttatgat gtgccatcag agtcgaactc catctagttt
      181 gtttttatcg agtgaaccaa accgtgcatg aaccggaggg gggagacgac aagaaggcaa
      241 gaggagagag gcagcaatcc aggtactttc ttcttgttat cccagtattt acccgaccaa
      301 tagacgcacc ttcgcacttt ggttagcaaa aaatatatac gtttatttac agaacgtgaa
      361 attttcttaa tacatttttt caacttggcc tcgaatcgcc tgatttttgg cagatctgcc
      421 cggctttaga gggaaagcag ttagaagtgg cgggttttta ttatgtttag cgctttctgt
      481 tccgcacttg tacaatgcct gccatgtctg cagatccgat agtcaattcc cttaatgaag
      541 cccagcgtcg tgcggtgacg tccgacgccg atcgcctgct ggtgctggcc ggcgccggca
      601 gcggcaagac ccgcgtgctg gtgcatcgca ttgcctggct gatccaggcc cgccatctgg
      661 cgccacacaa cattctggcg gttaccttca ccaacaaggc cgcagcggaa atgcgccacc
      721 gcatcgaatc cctcttgcgg gcacccttgg gggcaatgtg ggtaggcact ttccacggta
      781 tcgctcaccg tttcctgcgc ctgcactggc aggaggccaa actgccgcag aatttccaga
      841 tcctcgacag cgacgaccag ttgcggctgg tgaagcgcgt gatgcgcggc atggagctgg
      901 atgaaacccg ctggccgccg aagcaggcgc agggcttcat caatgcgaac aaggatgaag
      961 ggctgcggcc tgagcacatc gatgactacg gggatattca ccagaaaacg ctggtgaaga
     1021 tttaccagca ataccaggat gcctgcgaaa ccggcggcat ggtggatttc gccgaactgc
     1081 tgctgcgcag ccatgaactc tggctcaagc atcccgcctt gctgcagcat taccgccagc
     1141 gtttccgcca cgtactggtg gacgaattcc aggataccaa tcgcatccag tacgcctggt
     1201 tgcgggtgct ggtgggtaac gacaacgcga tcacgattgt gggtgacgac gatcagtcca
     1261 tttacggctg gcgcggcgcg cgggtggaga acatccgcca gttcggcgac gatttcggcc
     1321 gcgccgaggt ggtgaggctg gaacagaatt accgctctac gtccattatc ctgaatgctg
     1381 ctaacggcgt gattgcgcac aacagcgatc gtctcggcaa gaatctgtgg accagcggtg
     1441 ctgagggtga ccccatctcg gtttatgccg gtttcaacga agtggatgaa gcgcgcttca
     1501 tcgccgagcg cattcagcag gcgctgcaac tgggccatcg gcgagacgat attgcaattt
     1561 tgtatcgatc caacgcgcag tcgcgggttc tggaagaggc gctgttgcgc gcggccattc
     1621 cctaccgcat ttacggcggc caccgttttt tcgaacgcgc cgaaatcaag aatgcgctgg
     1681 cctatctgcg tctgatctgc caccgcgatg acgatgctgc cttcgagcgg gtggtgaaca
     1741 cgcccacgcg cggtctgggg gacaaatccc tggagcgcat ccgtgcgctg gcgcgggaac
     1801 tgaaaattcc gctgtggcag gcgagcctgc aggtgctgca acaccgcgag ctggccacca
     1861 aggctggcaa tgcgctgaaa gggtttctgg cgctgatcga acagctggat caggagacac
     1921 gaggcaccgg cttgtcggaa caggccgagc gggtaattga ggcttccggt ctgatcgcct
     1981 tccatcagaa tgaaaaagac gatcgcggcg aagcgcgggt ggagaacctg aaggaactga
     2041 tcagcgccac gcgggaattc gagatttcac tgcagcagga aagcgccaca ctggcggcgt
     2101 tcctggatca tgcggcgctg gaggcgggcg aaacccaggc cggcgagaac gaggactccg
     2161 tgcagatgat gacgttgcac gcggcgaagg gactggaatt ccccatcgtg ttcatggcgg
     2221 gggtggaaga aggtctgttc ccgtccatgc agtcggcgga cgatccgcat cgcatggagg
     2281 aggagcgccg cctgtgttac gtcggcatca cccgggccat gaaaaatctt tacatcacct
     2341 acgcggaaag ccggcgcatt tacggttcgg aagccttgca tgcgccgtcc cgtttcattc
     2401 gcgagatccc gctgcacctg ctgcaggaag tacgggcgtc ggcgcggctg acccgggcca
     2461 tgtcgacgtt cgattccgga tttggcggtg tgcgcgagcg ttccgccgga tatcgcagta
     2521 atacctatgg tggtgcgcgc atccaggcgg acgattcacc gtatcagctg ggccggtccg
     2581 tgcgccatcc caaattcggt gacggtgtga tcatggggtg cgaagggcaa ggccccaatg
     2641 cccgtgtgcg ggtgaatttt cgcggtgaag gcgagaaatg gctggtagcc cagtacgctc
     2701 ggctggagtt cgtttgaggc gttgcccggc gctgtaatcg tcacgcgcac cggctacagt
     2761 gcgcgcgacg atggtggtca ggccgtgcgg ggtaccacgc gggtgcgacg gttctcgtca
     2821 atcgccacga aggtgaactg gccttcggtg accttgcggc attcagtgtc ggaatggtgc
     2881 tgggcccaga cttccacccg gatggtgatc gagctgcgac cgatgttcag tacctcggta
     2941 tggcagctca cgaccgaacc cactgccacc ggccgcagga agaccatcga atcgattgcc
     3001 acggttgcta cccggccccg ggccaggcga ttggcggtga cagcaccggc caggtccatc
     3061 tgcgacacca gccagccccc gaaaatgtcg ccgtcggcat tggcgtcggc cggcatggcg
     3121 atggtttgca gcgccagttc ccccaccgga gtggggatgt cgtcaatctg ttgttcgtcc
     3181 attttgttgt ccacgctttg tactacctag agttatagac caaatccagc aaccggttgg
     3241 cctgtgtcag cctcccggga atgatgccca aacggtaaca cgccccggca gattgtcgaa
     3301 gtggaaaagg ggcgggtttg tgtcctgttg cctgcgggcg gtcggcgggc gttggtcagt
     3361 cctatttttg taaccagggg gaaatagtct tgtccgggac tggatgttca ttataacggc
     3421 agccttgcgg gcctagcaaa agcgctttgt gtccatttgc aggggggctg gcggcaacag
     3481 atctgtaata taattgtcac gaagccgtcc tatagttccg agcccagaag ggagagaccc
     3541 atccccccat gctcccttgg gaacacttac catctcggag aagctcatga atttcaagaa
     3601 aatgattgca gcagtcagcc tgactgccgg tgcggccatg ctggtgtccg ctgcccacgc
     3661 agcaacgaat gttgatccca agctgcccgc ttacgccaag gttggtggca tttccggcaa
     3721 cctgtccagc gtcggttccg acaccctggc caacctgatg acgttctggg cggaagattt
     3781 caagaaggct taccccaatg tggcggtcca gattcaggct gccggctcct ccacggctcc
     3841 cccggcgctg accgagggca ctgccagctt cggccccatg agccgtccca tgaaggatca
     3901 ggaaatcgaa gccttcgaaa agaaattcgg ctacaagccc accgcagtgc cggttgcgat
     3961 tgacgccctg gcggtcttcg tccacaagga caacccgatc accggcatga ccatggaaca
     4021 ggttgacgcc gtgttctcca acacccgcac ctgcggcgcc agcaaggacg tgtccaagtg
     4081 gggcgatctg ggcctgaccg gtgcctgggc tgcccgtgac ctgcagatgt acggccgcaa
     4141 ctcggtatcc ggcacctacg
//
LOCUS       DASUEG010000057         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_836272, whole genome shotgun sequence.
ACCESSION   DASUEG010000057 DASUEG010000000
VERSION     DASUEG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836272"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1653
                     /locus_tag="VFX11_00955"
     CDS             190..1653
                     /locus_tag="VFX11_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4937214.1"
                     /translation="MGFTPQQQNVNSPIDGSSLAKIGYASPDKINALVSEAQHCFMDW
                     RQLPAPRRGELVRVMGEIARECKTYLARFIVLEAGKTMQEALGEVQELIDICDFAVGL
                     SRQLTGLTIASERPQHRMMEQWHPLGVVGIITAFNFPMAVWAWNATLALVCGNTLVWK
                     PSEKTPLCALACQQLLQEAVKTCGMDPFESLSSVIVGGADVGEALAAHPQVALVSATG
                     SVAMGRSVAQTVAARLGRSLLELGGNNGIIVSKKADLDLALKGVTFSFVGTSGQRCTS
                     LRRLFLHESIANYFETKLIAALKTLRIGDPRDEQMLCGPLIDKGSFTRFQQAIKAAQA
                     QGGHLLYGGDRIKADVPAGGYYVNPAVIAIAPDAEILQQETFAPLLYVVRFLDLRQAL
                     DWHNNVPQGLSSAIFTNDLREAEQFLAVTGSDCGIANVNIGTSGAEIGGAFGGEKDTG
                     GGRESGSDAWKNYMRRSTNTINYGTDIPLAQGIRFEL"
     gene            1742..>2120
                     /locus_tag="VFX11_00960"
     CDS             1742..>2120
                     /locus_tag="VFX11_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX4937215.1"
                     /translation="MAKKNVDVLLGFGGVHPQENARALYWGEKLEIPMLLMALWIVLE
                     WYLSAKGVMSPHDRDISDWFVWTFFLIETSCLVYLCDDKWRHLKRNWANLVIIASAIP
                     FLQYTVHELGALRFLRLLFLVGLF"
BASE COUNT          406 a          562 c          704 g          448 t
ORIGIN      
        1 gaagatgtgc gcctgtcgga ttttctggcc accgaatttg gcgaagtcta caaaacggcg
       61 gaaccgatcg tgcatgcctg aaggcacaca caggagaaca acaacatggt atggaatgca
      121 gcgattcagg aattggcaga attctggcac gccgatcagg cgtttcccgc ctgggcctgc
      181 ggcagccgga tgggattcac cccgcagcag cagaatgtaa attcacccat cgatggcagc
      241 agtctggcga agatcggtta tgcgtccccc gacaaaatca atgcgctggt gagcgaagcc
      301 cagcactgtt tcatggactg gcgccaattg ccggcgcccc ggcgtggcga gctggtgcgg
      361 gtgatgggcg agattgcgcg cgaatgcaaa acctatctgg cccgtttcat cgtgctggaa
      421 gcgggcaaga cgatgcagga agccctgggt gaggtgcagg agctgatcga catctgcgat
      481 ttcgcggtgg gcctgtcgcg ccagctcacg gggttgacca ttgccagcga gcggccccag
      541 caccggatga tggagcagtg gcatccgctg ggcgtggtcg gcatcatcac cgccttcaat
      601 tttcccatgg ccgtgtgggc gtggaatgca acgctggcgc tggtgtgtgg caatacgctg
      661 gtgtggaagc cgtcggagaa aacgccgctg tgtgcgctgg cctgccagca actgttgcag
      721 gaagcggtga aaacctgcgg catggacccg tttgaatcgt tatcgagtgt tattgtcggt
      781 ggcgcggacg tgggtgaggc gctggcagcg catccgcagg tggcgctggt gtcggcgacc
      841 ggttcggtgg cgatggggcg cagtgtggcg cagacggtgg cagcgcggct gggccgcagt
      901 ctgctggagc tgggcggcaa caacggcatc atcgtgtcga agaaagcgga tctggatctg
      961 gccctgaaag gtgtgacctt ttcgttcgtt ggcaccagcg gccagcgctg taccagtctg
     1021 cgccggctgt ttctgcacga atccattgcc aattatttcg aaaccaaact gatcgcggca
     1081 ctgaaaacct tgcgcatcgg cgatccccgc gacgagcaga tgctgtgtgg tccgctcatc
     1141 gacaagggat catttacccg cttccagcag gcgatcaagg cggcgcaggc ccagggtggg
     1201 cacttgctgt atggcggcga ccgcattaag gctgatgtgc cggccggtgg ctattacgtg
     1261 aatcctgccg tgattgccat tgcaccggat gcggagatcc tgcagcagga aaccttcgcg
     1321 ccattgctgt atgtggtgcg gtttctcgac ctgcgccagg cgctggactg gcacaacaat
     1381 gtgccgcagg gattgtcgtc ggcgattttc accaatgacc tgcgggaggc ggagcagttt
     1441 ctggcggtga ccggatcgga ttgcggcatt gccaacgtca atatcggtac ctctggcgcg
     1501 gaaattggcg gcgcgtttgg cggcgagaag gataccggcg gcgggcggga atccggatcg
     1561 gatgcctgga aaaactatat gcgccgcagc accaacacca tcaactatgg cactgacatc
     1621 ccgctggcgc agggtatccg ctttgagctc tgacgtcgcg ttgttgcccg cagtcaagat
     1681 ggaatccttg ggacctctgt tatagtagcg ccttcctttt tgctgagcgg tgtggacagg
     1741 aatggccaag aagaacgttg atgtactgct gggattcggc ggcgtgcatc cccaggaaaa
     1801 tgcgcgggca ctgtattggg gggagaaact ggaaatcccc atgctgctga tggcgctctg
     1861 gatcgtgctg gagtggtacc tgtcggccaa gggggtcatg agcccgcatg accgcgatat
     1921 cagcgactgg ttcgtctgga ccttttttct gatcgaaacc agctgccttg tgtatctgtg
     1981 cgatgacaaa tggcggcatc tgaaacgcaa ctgggccaat ctggtgatca tcgcgtctgc
     2041 tatcccgttt ctgcaatata cggtacatga gctgggtgcc ctgcgtttcc tgcggctgct
     2101 gttcctggtg gggctgttcg
//
LOCUS       DASUEG010000058         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_662227, whole genome shotgun sequence.
ACCESSION   DASUEG010000058 DASUEG010000000
VERSION     DASUEG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662227"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2950
                     /gene="glnE"
                     /locus_tag="VFX11_00965"
     CDS             <1..2950
                     /gene="glnE"
                     /locus_tag="VFX11_00965"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX4937216.1"
                     /translation="KTCCMSLTLESLATLSPAVHAHVDQLLLRLTEAVHALPAPDADC
                     LTETRVQAQIVPVLVASDYVATQLQRHPQLLAELVRSQDLWRSYDDHVYHASLQSLLQ
                     DVNDETVLMKILRQLRRREMVRIIWRDSTGLAPFAETARDLSNLADACIDQTLQKLYP
                     ILEPLWGRPRYKQSGDPMPMVVLGMGKLGAQELNLSSDIDLMFAYAHDGETSGGARSL
                     DHREYFLRLGQRLIKALDENTADGFVFRVDMRLRPFGASGPLAMSFASMENYYEDQGR
                     EWERYAMIKARAIGTGTALGDGEKLLLALRPFVYRRYVDFGVIESLRELKNLINQEVL
                     RRGKEDNVKTGAGGIREIEFIAQVFQLIRGGQEQELQRRNLLTVLNVIRDLELLPHAI
                     VKELRDDYIFLRNVEHRIQALQDRQTQLLPDDDINRLRIAIGLGFASWETFYAHLEAV
                     RQRVRLHFSNLIADSNKDEAVSVRDQAVSLWMESESGPEALDDLRALGYRDVDDTWRL
                     LSEMKSSKQVTHLQAVARERLDKLMPLIINACGQQDNPDVALQRVLRLIEAVRRRSAY
                     MALLVENPRSLNRLAMLFSASPWVADTMCRYPLLLDTLLNEASLLKPLTVDVLSNELR
                     QQLLRIPEDDLERQMDTLRQFKHAHVLRVAASEMAGTLPLMKVSDYLTWLAETLLQSV
                     FDLAWRQMVEKHGRPQREKGVPCDPDFIIVGYGKVGGLELSYSSDLDLVFIHDAHPAL
                     ETDGTNAIDNAVFFARLGQRIIHILTTRMASGELYETDMRLRPSGNSGLLVSSLKSFG
                     EYQQDEAWTWEHQALVRARVLAGCPRLAQKFDAVRQAILGKPRDPAALLKDVTDMRLK
                     MLGQLSSHKGATDLDESLLRADETFDLKQDPGGIVDIEFLAQYLVLRWSGEKPAMARW
                     TDNMRILDAARDEGLLTVEEATALQQAYLTFRSESHRLALLNEPAKVTGDRFAEERRE
                     VRAIWHKTLG"
BASE COUNT          590 a          936 c          933 g          551 t
ORIGIN      
        1 gaaaacctgc tgcatgtcgc tcacactcga atccctggca acgctgtccc ccgctgtgca
       61 tgcccacgtc gatcaactgc tgctacggct gacggaggcg gtgcatgcgc tgccggcacc
      121 ggacgccgat tgcctgaccg aaacccgcgt gcaggcgcag atcgtgccgg tgctggtggc
      181 cagcgattat gtggcgacgc aactgcagcg gcatccgcaa ttgctggcgg agctggtgcg
      241 gtcacaggat ctgtggcgtt cctatgacga ccatgtttat cacgcatcat tgcagtcact
      301 gttgcaggac gtgaacgacg aaaccgtgct gatgaaaatc ctgcgtcaat tgcggcggcg
      361 ggaaatggtg cgcatcatct ggcgcgacag caccggtctg gcgccgtttg ccgaaaccgc
      421 ccgcgacctc agcaacctgg cggatgcctg catcgaccag acgctgcaaa aactctatcc
      481 cattctggaa ccgctgtggg gtcggccccg ttacaaacag agcggcgatc ccatgccgat
      541 ggtggtgctg ggcatgggca agctgggcgc gcaggaactg aatctttcat ccgacatcga
      601 cctgatgttt gcctacgccc acgatggtga aaccagcggc ggcgcacgct cgctggatca
      661 ccgcgaatat ttcctgcgcc tgggccagcg cctgatcaag gcgctggatg aaaacaccgc
      721 cgacggattt gtcttccgcg tcgacatgcg cttgcgtccg tttggtgcca gcggcccgct
      781 cgccatgagc ttcgcctcga tggaaaacta ttacgaggac cagggccgtg agtgggagcg
      841 ctacgccatg atcaaggccc gcgccatcgg cacgggaacc gcactgggcg atggcgaaaa
      901 actgctgctg gcgctgcgtc cgttcgtgta ccgccgctac gtggattttg gcgtcatcga
      961 atccctgcgc gaactgaaaa acctgatcaa ccaggaagtg ttgcggcgcg gcaaagaaga
     1021 caatgtgaaa accggcgccg gcggcattcg cgaaatcgaa ttcatcgccc aggtgtttca
     1081 actgattcgc ggcggccagg aacaggagct gcaacggcgc aacctgctca ccgtgttgaa
     1141 tgtgatccgc gatctggaat tgctgccaca cgccatcgtg aaggaattgc gcgacgatta
     1201 tatttttctg cgcaacgtgg agcaccgcat ccaggcgctg caggatcgcc agacgcagtt
     1261 gctgccggac gacgatatca atcgcctgcg cattgccatc ggtctgggtt ttgccagttg
     1321 ggaaaccttc tacgcccatc tggaagccgt gcgccagcgc gtgcgcctgc atttttccaa
     1381 cctgatcgcc gattccaaca aggatgaagc cgtcagcgtg cgcgaccagg cggtgagcct
     1441 gtggatggaa tccgaaagcg gcccggaagc gctcgatgat ctgcgcgcgc tgggctaccg
     1501 cgatgtggac gacacctggc gcctgctcag cgaaatgaaa tccagcaagc aggtgacgca
     1561 cctgcaggcg gtggcgcggg aacggctcga caaactgatg ccgctgatca tcaacgcctg
     1621 cggccagcag gataatccgg acgttgcgct gcagcgggtg ctgcgcctga tcgaggccgt
     1681 gcgccgccgt tccgcctata tggcgctgct ggtggaaaat ccgcgctcgc tcaaccggct
     1741 ggccatgctg ttcagcgcca gcccctgggt ggccgacacc atgtgccgct acccgctgtt
     1801 gctggatacg ctgctcaatg aagcctcgtt gctgaagccg ctcaccgttg acgtgctcag
     1861 caatgaactg cgccagcaat tgctgcgcat tcccgaagac gatctggaac ggcagatgga
     1921 caccctgcgc cagttcaagc acgcccacgt gctgcgtgtc gctgcgtcgg aaatggccgg
     1981 caccctgccg ctgatgaaag tcagcgacta cctgacctgg ctggcggaaa ccctgctgca
     2041 gagcgtgttc gatctggcct ggcgccagat ggtggaaaaa cacggccggc cgcagcgcga
     2101 gaagggcgtg ccctgcgatc cggatttcat cattgtcggt tacggcaagg tgggcggact
     2161 ggaactgagt tacagctccg atctggatct ggtgttcatt catgatgccc atccggcgct
     2221 ggaaaccgac ggtaccaacg ccatcgacaa cgcggtattt ttcgcccgtc tgggccagcg
     2281 catcattcac atccttacca cgcgcatggc ctccggtgaa ctgtatgaaa ccgacatgcg
     2341 cctgcgcccc tccggcaatt ccgggttgct ggtgagttcg ctgaaatcct tcggcgaata
     2401 ccagcaggac gaagcctgga cctgggagca tcaggccctg gtgcgcgccc gcgtgctggc
     2461 cggttgcccc cggctggcgc agaaattcga tgccgtgcgt caggccatcc tgggcaagcc
     2521 gcgcgatccg gccgcgctgc tgaaagacgt caccgacatg cgcctgaaaa tgctgggtca
     2581 gctcagcagc cacaagggtg caacggatct ggacgaatcc ctgctgcggg ccgatgaaac
     2641 cttcgacctg aaacaggacc ccggcggcat cgtcgacatc gaattcctgg cccagtatct
     2701 ggtgctgcgc tggagtggcg aaaaacccgc catggcccgc tggaccgaca acatgcgtat
     2761 cctcgacgcg gcccgtgacg aggggttgct gactgtggaa gaggccactg ccctgcagca
     2821 ggcctacctg accttccgca gcgagtcgca ccggctggcc ctgctcaacg agccggccaa
     2881 agtgaccggc gaccggttcg cggaggagcg gcgggaagtg cgggcaatct ggcataaaac
     2941 cctgggttga gcctcgtcaa aggccttgcc acttgtgcta tagtggccgg cctcagccgc
     3001 ggccgacgcg
//
LOCUS       DASUEG010000059         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1852808, whole genome shotgun sequence.
ACCESSION   DASUEG010000059 DASUEG010000000
VERSION     DASUEG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852808"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1002)
                     /locus_tag="VFX11_00970"
     CDS             complement(121..1002)
                     /locus_tag="VFX11_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX4937217.1"
                     /translation="MKFRVKHTTHYRYSSPVSLCFNEARMSPLNNTHQTCARNRFVIH
                     PAPDSVHKRFDYFGNQVTAFDILQQHKELTVTVYSDVQVLDPSRQFLFSADCPWEHVR
                     DSLRTTTQADLLPVREFVLPSPLLPALDEMRAFAEKSFLPQRPVLEAAMDLMNRIHTE
                     FTYDPSATTISTPLATVLAQKRGVCQDFAHLGIACLRSMGLAASYISGYLETLPPPGQ
                     KKLVGVDASHAWYAVHMLDDGWVAFDPTNNIMPTDQYITLAIGRDFADVTPLKGVIYG
                     GDHHLLKVSVDVDRIEA"
     gene            complement(999..3614)
                     /locus_tag="VFX11_00975"
     CDS             complement(999..3614)
                     /locus_tag="VFX11_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX4937218.1"
                     /translation="MTTDIKMKQSQSQTAAETPSELEQMLAVYPAGLDGYDEMWDAGS
                     ATVNPQWRRLMRDLQELGGDELERRRTEARRLLRENGVTYNIYNDPKGQQRTWQLDPI
                     PLLIQSADWAAISVGLKQRARLLDMVYRDLYGARRLIREGLLPPELIYAHQGFLRPCD
                     QVPQENMPQLLLYAADLARGPDNRMWVIGDRTQAPSGAGYALETRMAMTRVMADEFRR
                     TQVHRLSIFFRNLRATLASLLPNYEEEPRVVILTPGPRNETYFEHAYLASYLGYSLVQ
                     GDDLTVRDGKVWLKSLDGLKKVDIIVRRVDDTFCDPLELREDSHLGVPGLLQAARLGN
                     VIIANPLGSSLLESPGIMPFLPAIAKHWLGEEILLPSAATWWCGQKKEMDYVLEHMPD
                     LVIKTIDRRMPSFFGPQLSSAQRAELKARITAMPYLFVGQELLSCSTAPSLREAAIVP
                     RRVLLRAFVTAHQGDYDVMPGGLSRVAPDSDSLFISNQSGGLSKDTWILADKPVHQVS
                     LWPSVRQMDSLVTGEGSLPSRSGENLFWVGRYAERAEGLLRLLRTVIGKLADYDDYRD
                     EADMAVLTQLLVTLTHFTSIYPGFIGEEAKESLANPREKILQMALDRNQRCSLISTLE
                     SFMQSAYNVRDLWSVDTWRLIDDIDENIQYLGGKSDDFGLLQNELDSLIGTLMAIFGQ
                     NQESMPHDAGWYLLSMGRRIERCLQQIILLRTCLLEPSGDVVEHLMLESVLINQASLI
                     THRRRYRALQHVYTVLDLLLLDSQYPRSLAYQVEQIGQLLAKLPPVQKNLPPHQLSRE
                     QRLILQASTEIKLAEVEKLGRISEETGSRIHLAKLLDNVERRLAKASALLTQKYFSHT
                     ESARQLAPTRMDLSV"
BASE COUNT          657 a         1074 c         1207 g          736 t
ORIGIN      
        1 cgcgcaagtg tggccgacga actgcgcggc gtgccgttgg tttgaatcag ttcgtcgaaa
       61 aaatctttta cttcgtaatc tttccagtcg atgggcataa atcgtcttat aggtgaaaag
      121 tcacgcttcg atacgatcca catccaccga cactttcagc agatgatgat cgccgccgta
      181 tatcacgcct tttaagggtg tgacgtctgc aaaatcccgg ccaattgcca gagtgatgta
      241 ctggtccgtg ggcatgatgt tgttggtggg atcgaaggcc acccagccgt catccagcat
      301 gtgcacggcg taccaggcgt gggatgcatc cacccccacc agttttttct gccccggcgg
      361 cggcagggtt tcgagatagc cgctgatata gctggccgcc agccccatgc tgcgcaggca
      421 ggcgatgccc aggtgggcga agtcctggca cacaccccgt ttctgcgcca gtaccgtggc
      481 cagcggcgtg ctgatggtgg tggcgctggg gtcataggtg aattcagtgt gaatgcggtt
      541 catcagatcc atcgcagcct ccagcaccgg gcgctggggc agaaaggatt tttcggcaaa
      601 cgcccgcatc tcgtccaggg cgggcagcag tggcgacggc agcacgaatt cccgtaccgg
      661 cagcaggtcg gcctgcgtgg tggtgcgcag gctgtcgcgc acgtgttccc acgggcagtc
      721 ggcgctgaac aggaactggc gcgaggggtc gagcacctgc acgtcgctgt atacggtgac
      781 ggtcagttcc ttgtgttgct gcaggatatc gaaggcggtg acctggttgc cgaagtaatc
      841 gaagcgcttg tgcaccgaat cgggtgccgg gtgaatcaca aaacgattgc gcgcacaagt
      901 ttggtgcgtg ttgttcaggg gcgacatgcg ggcttcattg aagcagaggg aaaccgggct
      961 gctgtagcgg taatgggtgg tgtgtttgac gcggaatttc ataccgacag atccatgcgg
     1021 gtgggcgcca gctggcgtgc gctttcggtg tggctgaagt atttctgggt cagcagggcg
     1081 gaggccttgg ccaggcggcg ctcaacattg tccagcagtt tggccagatg aatccggctg
     1141 ccggtttctt cgctgatgcg accgagcttt tccacttccg ccagcttgat ttcggtgctg
     1201 gcctgcagga tcaggcgctg ttcacggctt agctgatgcg gcggcaggtt tttctgtacc
     1261 ggcggcagct tggccagcag ctgtccgatc tgttccacct ggtaggccag cgaacgcggg
     1321 tactggctgt ccagcagcaa caggtccagc acggtgtaga catgctgcag cgcacgatag
     1381 cgacgacggt gggtgatcag gctggcctgg ttgatcagca ccgattccag catcaggtgt
     1441 tccaccacgt cgccgctggg ttccagcaga caggtgcgca gcaggatgat ctgctgcagg
     1501 cagcgttcga tgcgacggcc catgctcagc agataccagc cggcatcgtg gggcatgctt
     1561 tcctggttct ggccgaagat cgccatcagg gtgccaatca gggaatcgag ttcgttctgc
     1621 agcaggccga aatcatcgga cttgccgccg aggtactgaa tgttctcgtc gatgtcgtca
     1681 atcaggcgcc aggtgtccac cgaccacagg tcgcgcacgt tgtaggcgct ttgcatgaag
     1741 gattccagcg tgctgatcag gctgcagcgc tgattgcggt ccagtgccat ctgcaggatc
     1801 ttttcccgtg gattggccag ggattccttg gcttcctcgc cgatgaaacc ggggtaaatc
     1861 gaagtgaaat gggtgagggt gaccagcagc tgggtcaaca ccgccatgtc cgcttcgtca
     1921 cggtaatcgt cgtagtccgc cagtttgccg atcacggtgc gcagcagccg cagcaggcct
     1981 tcggcgcgtt cggcatagcg gcccacccag aacaggttct cgccggagcg tgacggcagc
     2041 gagccttcgc cggtcaccag gctgtccatc tggcgcacgc tgggccacag gctgacctgg
     2101 tgcaccggtt tgtccgccag aatccaggtg tctttcgaca ggccgccgga ctggttggag
     2161 atgaacaggc tgtcggaatc cggcgcgacg cggctgaggc caccgggcat cacgtcgtag
     2221 tcgccctggt gcgcggtgac aaacgcccgc agcagcaccc ggcgcggcac gatggccgct
     2281 tcccgcaacg acggcgcggt ggagcagctc agcagctcct gaccgacgaa cagatagggc
     2341 atcgcggtga tgcgggcctt cagttccgcg cgctgggcgc tgctcagctg cgggccgaag
     2401 aacgacggca tgcggcggtc gatggtcttg atcaccagat ccggcatatg ctccagcaca
     2461 taatccattt cctttttctg gccacaccac caggtggcgg cggagggcag caggatttcc
     2521 tcgcccagcc agtgtttggc gatggccggc aggaagggca tgatgccggg actttccagc
     2581 aggctcgatc ccagcgggtt ggcgatgatc acattgccca gccgcgccgc ctgcagcagg
     2641 cccggcacgc ccaggtgaga atcctcgcgc agttccagcg gatcgcagaa ggtgtcgtcc
     2701 acccggcgca cgatgatgtc gacctttttc aggccatcga gggatttcag ccacaccttg
     2761 ccgtcgcgta ccgtgagatc gtcgccctgc accaggctgt agccgaggta ggacgccaga
     2821 taggcgtgct cgaaataggt ttcgttgcgc ggaccgggcg tgaggatcac cacgcgcggt
     2881 tcttcctcgt aattgggcag caggctggcc agggtggcgc gcaggttgcg gaagaaaatc
     2941 gacaggcgat ggacctgggt gcgacggaat tcatcggcca tcacccgggt catggccatg
     3001 cgggtttcca gcgcatagcc cgcgccggag ggcgcctggg tgcggtcacc aataacccac
     3061 atgcgattgt cggggccgcg ggccagatcc gccgcgtaaa gcagcagctg gggcatgttt
     3121 tcctgtggca cctgatcgca ggggcgcagg aagccctggt gcgcgtagat cagttccggc
     3181 ggcagcaggc cctcgcggat caggcgacgc gcgccgtaca gatcacggta gaccatgtcc
     3241 agcagacggg cgcgctgctt caggcccaca ctgatggcgg cccagtcggc ggactggatc
     3301 agcaggggaa tcgggtccag ctgccaggtg cgctgctggc ccttgggatc gttgtagatg
     3361 ttgtaggtga cgccgttttc ccgcagcaga cgacgcgctt ccgtgcggcg ccgttccagt
     3421 tcgtctccgc caagttcctg cagatcccgc atcagccggc gccattgggg attcaccgtg
     3481 gcggagccgg catcccacat ttcgtcgtag ccatccagtc cggcgggata aaccgcgagc
     3541 atctgctcca gttccgacgg cgtttccgcc gccgtttgcg actgcgattg cttcattttg
     3601 atgtctgtgg tcatgcctgg ttgcgcccag agtcgtgccg atgccaaggt gtgaagtata
     3661 acactcaggg cgta
//
LOCUS       DASUEG010000060         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_107292, whole genome shotgun sequence.
ACCESSION   DASUEG010000060 DASUEG010000000
VERSION     DASUEG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107292"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFX11_00980"
     CDS             <1..487
                     /locus_tag="VFX11_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX4937219.1"
                     /translation="GKSRPTLAAKSLVTFTRISRTAASGMDEYDPKKWMGAVRNRTSD
                     RYNEAVLDDRIGLQVCDAGAGIVEVALTPYVGNSIGTISGGVQAITMEAAANAMRPGM
                     TATDIQIHFLSQLKVGPSRTRGTIVRDARDHSVVLIELLDAGAGNKLLATATLTLQRL
                     S"
     gene            complement(502..813)
                     /locus_tag="VFX11_00985"
     CDS             complement(502..813)
                     /locus_tag="VFX11_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX4937220.1"
                     /translation="MSKEFARFCEASALPDGSKRAKKINDTMVLVCHTQGRLFAVSIV
                     CSHQDKFLHAGRVRNCKITCPLHGAQFDLATGKATCLPATKPIPTYEVRVVDDWIEVC
                     V"
     gene            complement(830..2221)
                     /locus_tag="VFX11_00990"
     CDS             complement(830..2221)
                     /locus_tag="VFX11_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4937221.1"
                     /translation="MTVKIKIDNGEARCPNAPSTQDIIARDKVGAPDWVRSESYQFLG
                     NEDISTDRYTDPAFAKKELNTLWKKTWQFACREEHIPSVGDYYVYDLEHYSFIVTRVS
                     KDEIRAYYNACLHRGTKLRASGTEGCANEFRCPFHGWSWNIDGTNKNVVCDWDFPHVD
                     RNDLCLPQARVETLGGFVFVNMDENAPSLPDYIGPEAMAHITRWKLEDRYIVAHVAKV
                     IPANWKLTIEAFMEAYHVLETHPQVAPSNGDANSQYDIFGEHVDRFISTLGVVSPHLK
                     GRISEQDVLDQFTVGDSSVLQGADRKLKEGETARQRTADMMRSMFEKATNTDLSNVSD
                     SELLDCFSYTVFPNTFLFPGISLPMVYRFRPVKGDHRKCLYEVFFLRPVPKDGNRPEP
                     ACEVRLQEHQSFTEAEGMDPGFGAILDQDTENLLLEQEGLEASAKKGITLGNYQEIRI
                     RHFENTVDKYVNG"
     gene            complement(2258..>3211)
                     /locus_tag="VFX11_00995"
     CDS             complement(2258..>3211)
                     /locus_tag="VFX11_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4937222.1"
                     /translation="TSAWGASEAKLRLGVAVRNAVNAGHLKGPRIRAGSLEISVTGAM
                     GDESRLHDPRPGGPSVIVDGPEEMRKAVRLACREGCDNIKLDVSGDPFYPGTPANTTP
                     MTFEEVKMAVDTAHAYGRRVNAHVRSIEGAKVCLRAGVDALFHCEFTDEEALDLFEEA
                     KDRVFVVPTVSLFHTLLHEAEPYMSNAIARAMQIDRLLEASQDTHNALRKRGIRHTIG
                     GDYGFAWSHNGTNARDIGFFVQYFGYSPKEALVCATANGGAMMTMGTDEKLGLIKEGY
                     LADLLLVKGDPIADPAVVVNASNLKLIMKGGSLHKNTVAAG"
BASE COUNT          644 a          865 c          967 g          735 t
ORIGIN      
        1 cggtaaatcg cggccgacac tggcggcaaa aagtctggtg acctttacca gaatctcccg
       61 caccgcggca agcgggatgg atgagtacga tccgaagaaa tggatgggtg cggtacggaa
      121 cagaacatca gaccgttaca atgaagctgt gctggatgac cgcatcggcc tgcaagtgtg
      181 cgatgcagga gcgggaatcg tcgaggttgc gctgacgccc tacgtgggca acagcattgg
      241 cacgatcagc ggcggtgtgc aggccatcac gatggaagct gctgccaacg ccatgcgacc
      301 tggcatgacc gcgaccgata tccagatcca cttcttgtcc caattgaaag tgggtccgtc
      361 gcgaacccgg ggaaccattg tcagagacgc ccgggatcac agtgtcgttt tgattgaatt
      421 gctggatgcc ggtgcaggca acaagctgct cgcaaccgcc acactgacct tgcaacggct
      481 gtcgtagcta tttcgcgttt gtcacacgca aacttctatc cagtcatcca ctacgcgcac
      541 ttcataagtc ggaatgggtt tggtggcggg caggcaggtg gcttttccgg ttgcaagatc
      601 aaactgcgcc ccatgcagcg ggcaggtgat tttgcaattg cgtacccgac ccgcatggag
      661 gaatttgtct tgatgggagc agacgatgga aaccgcaaac agccggccct gggtgtgaca
      721 caccaggacc atggtgtcgt tgattttctt cgcccgcttg gaaccatcag gaagcgctga
      781 ggcttcacaa aaacgcgcaa attctttaga caaaatcaaa ttcctgctgt tatccgttga
      841 cgtatttgtc cactgtgttt tcgaagtggc gaatacggat ttcctggtag ttgcccagcg
      901 taataccttt tttagcactg gcttccaggc cttcctgttc cagcagcagg ttctcggtgt
      961 cctggtcgag gatggcgccg aatccggggt ccatgccttc agcttccgtg aacgattgat
     1021 gttcctgcag acgaacctca caagcaggct ccggacggtt gccgtccttg ggtaccggac
     1081 gcaggaagaa aacttcatac aggcatttgc gatgatcccc tttcaccggg cggaagcgat
     1141 acaccatcgg cagggagatg ccggggaaca ggaaagtgtt gggaaacacg gtgtaggaaa
     1201 agcaatccag cagctcggaa tcggatacgt tgctcagatc ggtgttggtc gccttttcga
     1261 acatgctgcg catcatgtct gccgtgcgct gacgtgcggt ttcaccttcc ttcagtttgc
     1321 gatccgcacc ttgcagtacg gagctgtcac ccactgtgaa ctggtccagc acatcctgtt
     1381 cggagatgcg gcctttcagg tgggggctga cgacgccgag cgttgaaata aagcggtcaa
     1441 cgtgctcacc gaaaatatcg tactgggagt tggcatcgcc attggagggc gcaacctgcg
     1501 ggtgggtttc cagcacatgg taggcttcca tgaacgcttc gatggtcagc ttccagttgg
     1561 ccggaataac cttggcgaca tgcgccacaa tgtagcggtc ttccagtttc cacctggtga
     1621 tgtgggccat ggcctcggga ccgatatagt cgggcagtga gggcgcattt tcgtccatgt
     1681 tgacgaatac gaaaccaccc agcgtttcaa cgcgagcctg gggcaggcac aggtcgttgc
     1741 gatctacatg ggggaagtcc cagtcgcaga cgacgttctt gttggtgccg tcgatattcc
     1801 aggaccagcc atggaacggg cagcggaatt cgttggcgca gccttcggtg cctgacgcac
     1861 gcagtttggt gccccggtgc aggcacgcat tgtagtaggc gcggatttcg tcttttgata
     1921 cgcgggtgac gatgaaggag tagtgttcca gatcatagac gtaatagtcg cccacggacg
     1981 gaatgtgctc ttcgcggcag gcgaactgcc aggtcttttt ccagagagtg ttcagttctt
     2041 ttttggcgaa cgcgggatcg gtatagcggt cggtggaaat gtcttcgtta ccgagaaatt
     2101 gataggactc ggaacggacc caatccggtg cgcccacttt gtctcttgcg ataatatcct
     2161 gggtgctggg agcattgggg caacgtgctt cgccgttgtc gattttgatt tttactgtca
     2221 ttgcactctc tccggaatct tcttgcgttc aatcggctca gccggcggcg acggtgttct
     2281 tgtggagcga accgcctttc atgatgagct tgaggttgct ggcgttgacc accactgcag
     2341 gatctgcaat cggatcaccc ttcaccagca gcaggtcggc cagatagcct tccttgatca
     2401 gcccgagttt ttcgtcggtt cccattgtca tcatggcacc gccgttggcc gtggcacata
     2461 ccagtgcttc cttgggtgaa tagccaaaat actggacgaa aaatccgatg tcgcgcgcgt
     2521 tggtgccgtt gtggctccag gcaaatccgt agtccccgcc gatggtgtgg cggataccgc
     2581 gcttgcgcag tgcgttgtgc gtgtcctggg atgcttccag caggcggtcg atctgcatgg
     2641 cccgtgcaat ggcgttgctc atgtagggtt cggcttcatg cagcagcgtg tggaacaggc
     2701 tgacggtggg aaccacgaat acgcggtcct tggcctcctc gaacaggtcg agcgcttctt
     2761 cgtcggtgaa ttcgcagtga aacagcgcat cgacaccggc ccgcagacac acttttgcac
     2821 cttcaatgga acgcacgtga gcgtttaccc tgcgaccgta agcgtgggcc gtatccaccg
     2881 ccattttgac ttcttcaaac gtcatcggtg tggtgttcgc gggtgtgccg ggatagaagg
     2941 gatcgccgga cacatcgagt ttgatgttgt cgcaaccttc gcggcaggcc agtcgcacgg
     3001 ccttgcgcat ttcttcggga ccgtcaacga tgacggaagg gccgccgggg cgcggatcat
     3061 gcaatctgct ttcgtcgccc atggcgccgg tgacggaaat ttccagcgaa ccggcgcgga
     3121 tgcgggggcc tttcagatgg ccggcattga ccgcattgcg gaccgcaacg cccagccgca
     3181 gtttggcttc ggatgcgccc caggcactgg t
//
LOCUS       DASUEG010000061         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2183912, whole genome shotgun sequence.
ACCESSION   DASUEG010000061 DASUEG010000000
VERSION     DASUEG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183912"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..446
                     /locus_tag="VFX11_01000"
     CDS             111..446
                     /locus_tag="VFX11_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX4937223.1"
                     /translation="MNVLQIDNRQIALDKEGFLENLADWSETVALALARREQIELTAA
                     HWEIIRALQDFYRQFEHSPPMRVLVKYVKQQLGDDKGNSIYLLQLFPGSPAKLAAKIA
                     GLPRPTNCL"
     gene            460..1473
                     /locus_tag="VFX11_01005"
     CDS             460..1473
                     /locus_tag="VFX11_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEX4937224.1"
                     /translation="MKAMKTSIPPSHEHPFAQFVRILGKGKKGSRPLTEDEAFQAMQM
                     IMQGEVLDVQLGAFLMLLRVKEETAEELLGFVRAVRSSVTPPQPLRVDLDWSSYAGKR
                     RHLPWYLFSIFLLAAHGHRVLVHGASGHTIDRIYTEDVLTDLGVPVAADWSSCAAQLD
                     QRNFSYFPLRNLCPRLHDIIELRNIMGLRSPVHTLSRLINPAGAPCVIQGIFHPGYRP
                     VHQVAALALGYSRVDVIKGEAGEIERNPDIECLVQSVRNGELVDETWPALFKQRHVKE
                     ESLDLTTFVKVWRGELHHEYGEAAVTGTLAIALKLLGQADSQEAAQTLANQWWQERDR
                     ALF"
     gene            complement(1565..>2608)
                     /locus_tag="VFX11_01010"
     CDS             complement(1565..>2608)
                     /locus_tag="VFX11_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937225.1"
                     /translation="VLITIVLIMVSLYVTMIVTGNIGRVVTSLQEIASGEGDLTKRIR
                     QNSQDEIGTLVYWFNSFVERLQGIIGDVVTSISPLSDVSKELGALATDSEHASSEQLE
                     ATVSVNRSMKEMFESLNENAVNTSNAAEAASDANDQAKKGHGIVSEAIRTINDLAREV
                     AKAGETIHQLEADTANVGQILDVIQGIASQTNLLALNAAIEAARAGEHGRGFAVVADE
                     VRTLASRTQESTEEIHRVIEQLQRTARAISEVMANGQRKAEESVAKAGETGSSLQAIT
                     SRVEEINVMNTQIASATEEQQQTSHFIQQAIDEISSSAQRAADGSAKVASSTEQLHKV
                     TQKLSAVAKQFRV"
BASE COUNT          523 a          791 c          739 g          555 t
ORIGIN      
        1 cggactggat ttgcctgcgc aggtgcagag cgtggattac aacggcttcg tcgatctggt
       61 cgcaaagttc gacaaatccg tatcctggtt ttagacggcg gttttccccg atgaatgtgc
      121 tgcagatcga caaccggcag atcgccctcg acaaggaagg ttttctggag aatctggctg
      181 actggagcga aaccgtggcg ctggcgctgg cgcggcgcga acagatcgaa ctgaccgcag
      241 cccattggga aatcatccgc gccctgcagg atttttatcg ccagtttgaa cacagcccac
      301 cgatgcgggt tctggtaaag tacgtcaaac agcaactcgg cgatgacaag ggcaacagca
      361 tttacctgct gcaactgttc cccggcagcc ctgccaaact ggctgcgaaa atcgccgggc
      421 tgccccgccc cacgaattgt ctgtagggaa tccgacatca tgaaagccat gaagaccagc
      481 attccgccgt cacacgaaca tcctttcgcc cagttcgtgc gcattctggg caaaggcaag
      541 aagggctcgc gcccgctcac cgaagacgaa gcattccagg ccatgcagat gatcatgcag
      601 ggtgaagtgc tggacgtgca gctgggcgcc tttctgatgc tgctgcgggt gaaagaggaa
      661 accgccgagg aactgctggg cttcgtgcgc gccgtgcgct ccagcgtcac accgccgcaa
      721 ccgctgcggg tggatctgga ctggtcgtcc tacgccggca agcgccgtca tctgccctgg
      781 tacctgttca gcatttttct gctcgccgcc catggccacc gcgtattggt gcacggtgcc
      841 agcggccaca ccatcgaccg catttacacc gaagacgtgc tgaccgatct gggcgtgccg
      901 gttgccgctg actggtccag ttgcgccgca caactggacc aacgcaactt cagctatttt
      961 ccgctgcgta acctgtgccc gcgcctgcac gacattatcg agttgcgcaa tatcatgggc
     1021 ctgcgctcgc cggtgcacac gctgtcgcgg ctgatcaatc ccgccggcgc accctgcgta
     1081 atccagggca ttttccaccc gggctatcgg ccggtgcatc aggtggcggc actggcgctg
     1141 ggttattccc gcgtggatgt gatcaagggc gaagctggcg aaatcgagcg caatcccgat
     1201 atcgaatgtc tggtgcagtc ggtgcgcaat ggcgaactgg tggatgagac ctggccggcg
     1261 ctgttcaagc aacggcacgt gaaagaggaa agcctggact tgaccacctt cgtgaaagtc
     1321 tggcgtggag aactgcatca cgaatacggc gaggcagcgg tgaccggcac actggcgatt
     1381 gcgctgaaac tgctgggaca agccgattca caggaagcag cgcagacgct ggcgaatcaa
     1441 tggtggcagg aacgggatcg cgcgctgttc tgaagttccg gcgcccatgc ctgccataaa
     1501 aaagcctcgc aacccttgca caggttgcga ggcttttttt gagccctgac aaagtacgcg
     1561 gtgcttaaac ccggaactgc ttcgccaccg cactcagttt ctgtgtcacc ttgtgcagct
     1621 gttcggtgga gctggccact tttgccgaac catccgctgc ccgttgcgcc gacgaactga
     1681 tctcatcgat cgcctgctgg atgaagtgcg atgtctgctg ctgctcttca gtagccgaag
     1741 caatctgggt gttcatcaca ttgatttcct cgacgcggga cgtaatggcc tgcaacgagg
     1801 aaccggtttc gccggctttc gccacgctct cttccgcctt gcgctggccg ttggccatga
     1861 cttcggaaat ggcgcgggcg gtgcgctgca attgctcgat cacacgatgg atttcttctg
     1921 tggattcctg ggtacgtgac gccagcgtgc gcacttcatc tgccaccacc gcaaacccgc
     1981 ggccatgctc accggcacgg gccgcttcaa tggcagcatt caacgccagc agattggtct
     2041 gcgacgcgat accttgaata acatccagaa tctggccgac gttggcagta tcggcttcca
     2101 gctggtggat ggtttcaccg gccttcgcca cttcccgcgc cagatcgttg atggtgcgaa
     2161 tcgcctcgct cacgatgcca tggcccttct tcgcctggtc attcgcatca gacgccgctt
     2221 ccgcagcatt ggacgtattc accgcatttt cattcaggct ttcgaacatt tctttcatgg
     2281 agcgattcac gctgaccgta gcttccagct gctcggacga cgcgtgctcg ctatcagttg
     2341 ccagggcacc caattccttg ctgacatcac tcaacggcga aatactggta accacgtcgc
     2401 cgatgatgcc ctgcaagcgt tcgacgaagg aattgaacca gtacaccagg gtaccgattt
     2461 catcctggga attctgcctg atgcgtttgg tcagatcacc ttcgccgctg gcgatttcct
     2521 gcagcgacgt caccacacgg ccaatattgc cggtcactat catcgtgaca tacagcgata
     2581 ccatgatcag cacgatggtg atcagcac
//
LOCUS       DASUEG010000062         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_990869, whole genome shotgun sequence.
ACCESSION   DASUEG010000062 DASUEG010000000
VERSION     DASUEG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990869"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFX11_01015"
     CDS             <1..735
                     /locus_tag="VFX11_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4937226.1"
                     /translation="GLKTLLIDLDPQCNSTQYLLGRDFQTGESDVGIFFAQSLTFRHR
                     NSKNLEDFITPSRYENLHVIAANPELGEIMSQLEAKHKIFKLRDALASITDYDAIYID
                     TPPAFNFYTLSALIAADSCLIPFDCDEFSRQALYNLIENVEETRADHNESLEVEGIIV
                     NQFQPRASLPTRMVDELKEEGLPILDRFISSSIKMRESHDLGVPLIYMAPKHKLTQEF
                     VELFELLHPGFAAAKKTDEEVESETA"
     gene            complement(851..2317)
                     /locus_tag="VFX11_01020"
     CDS             complement(851..2317)
                     /locus_tag="VFX11_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4937227.1"
                     /translation="MKQFLMSALLAGSALVAHAQEEPAALTDSVTHKLFQVQSQVLPN
                     GLHVIRHHRDTSDTFTVQLVVEVGLQDFPCKTQQAPHLLEHMLFEGTQRYDRKALRQL
                     IRDHGGMSNGFTKEEYTHYTFDIHSDYPDIALDALYSMMSEPLFNPADMETSRQVIHA
                     ELGTSSNQLQLALSGKRVLTSIAKARIYSGSNLECPDLTTPEGISLEQIKTIFEQHYV
                     PANMTLILLGHFDDARIDALLQQTFARLPARAPTPRAPVEFAAINYNPLQEKRALFDP
                     EVDVHLFIPAVGSIDPAHNSYQIVAEYLGEQLFYDVRGQRGMAYTPQALVDNNSLYGY
                     LQATTRTSDRWYDDVVRLFQEKYAQIRQQGVPQADVERLRQKLILEFESKQRDNAELA
                     QVYRHYRHIIRARGSMPDLVAQLQQVDAAQVKADIDRHFPEKPLLAILRPPTTEEILL
                     RIGPPALLLGGIGVILLRWSHRRRMKASLAATNVPAKK"
     gene            complement(2314..>2745)
                     /locus_tag="VFX11_01025"
     CDS             complement(2314..>2745)
                     /locus_tag="VFX11_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937228.1"
                     /translation="ARYGIAWGTLGAAECCWLAARDYTLQRTQFGKPLAANQLVQKKL
                     ADMQTDIALGLNGALRMGRLMDAGQCAPELISLMKRNNCGKALDIARTARDMHGANGI
                     SDLYPVLRHAINLETVNTYEGTHDIHALILGRAQTGLQAFQ"
BASE COUNT          511 a          767 c          866 g          601 t
ORIGIN      
        1 ggcctgaaaa cgttgctgat cgacctggac ccccagtgca attccaccca gtacctgctg
       61 ggccgcgatt ttcagaccgg cgaatccgat gtcggtattt tcttcgcgca gtcgctcacc
      121 ttccgccatc gcaacagcaa gaatctggaa gatttcatca caccctcccg ctacgaaaac
      181 ctgcacgtga ttgctgcgaa ccccgaactg ggcgagatca tgtcgcagct ggaagccaag
      241 cataaaattt tcaagctgcg ggatgcgctg gcgtcgatca ccgattacga cgcgatctat
      301 atcgacacgc cgccggcttt caatttctac acactgtcgg cgctgattgc ggcggacagc
      361 tgcctgattc cgttcgactg cgacgagttt tcccgccagg cgctgtacaa cctgatcgag
      421 aacgtggaag aaacccgcgc cgaccacaac gagagcctgg aagtggaagg catcatcgtc
      481 aaccagttcc agccccgcgc cagtcttccc acgcgcatgg tggacgagct gaaggaagag
      541 ggcctgccga ttctggaccg gtttatttcg tcgtcgatca agatgcgcga atcccacgac
      601 ctgggcgtgc ccctgattta catggcgccc aagcataaac tgacgcagga attcgtggag
      661 ctgtttgagt tgctgcatcc gggttttgcg gcagcgaaga aaaccgatga ggaagtggag
      721 tcggagacag cctgaatttt tctgacaggc acgttgcggc aacaggtgtg tcgtacgctg
      781 cgaaaataaa aaagggttcg ccggcagatc tggcgaaccc ttttttgtgt cggcatgtcg
      841 atgcccgcac ttattttttt gccggtacgt tggtggctgc gaggctggct ttcatccgcc
      901 gccggtgcga ccagcgcagc aatataacgc cgatgccccc cagcagcagg gcaggcggtc
      961 caatgcgcag caggatttcc tctgtggtgg gcgggcgcag aatcgccagc aatggcttct
     1021 ccggaaaatg ccggtcgata tcggccttta cctgtgcagc atccacctgc tgcagctgcg
     1081 ccaccagatc cggcatggaa ccccgggcgc ggatgatgtg ccgatagtgc cggtaaacct
     1141 gtgccagttc ggcgttgtcg cgctgtttgc tttcgaattc caggatcagt ttttgccgta
     1201 gccgttccac gtcggcctgg ggcactccct gttgccggat ctgtgcgtat ttttcctgga
     1261 acagtcgaac gacgtcgtcg taccagcgat cgctggtgcg ggtggtggct tgcagatagc
     1321 cgtacagact gttgttgtcc accagcgcct gcggtgtgta ggccatgccg cgctggccgc
     1381 gcacgtcgta aaacaactgt tcccccagat attccgccac gatctggtag ctgttgtggg
     1441 caggatcgat gctgccgacg gcggggatga acaggtgcac atccacttcc ggatcgaaca
     1501 gggcacgctt ttcctgcagc ggattgtaat tgatggcggc gaattccacc ggtgcgcggg
     1561 gtgttggcgc acgggcgggc agtcgcgcga aagtctgttg cagcagtgcg tcgatgcggg
     1621 cgtcatcgaa atggcccaac agaatcagcg tcatgttggc gggaacatag tgctgttcga
     1681 agatggtttt gatctgctcc agcgaaatgc cttccggcgt ggtgaggtcg gggcattcca
     1741 gattgctgcc ggaataaatg cgcgccttgg cgatactggt gagcacccgt ttgccagaca
     1801 gcgccagctg cagctggttg ctgctggtgc ccaattcggc atgaatcacc tggcgcgagg
     1861 tttccatgtc cgccgggttg aacaggggtt ccgacatcat ggaataaagt gcatcgaggg
     1921 cgatgtcggg ataatccgaa tggatgtcga aggtgtagtg ggtgtactcc tccttggtaa
     1981 aaccgttgct catgccgccg tggtcgcgaa tgagttggcg cagggccttg cggtcgtacc
     2041 gttgcgtgcc ttcgaacagc atgtgctcca gcaggtgcgg tgcctgctgg gttttgcagg
     2101 gaaaatcctg cagccccact tccaccacca attgcacggt gaaggtatcg ctggtgtcgc
     2161 ggtggtggcg gatcacgtgc aggccgttgg gcagcacctg gctttgcacc tggaacagtt
     2221 tgtgggtgac ggaatcggtc agtgcggcgg gttcttcctg cgcgtgcgcg accagggcgc
     2281 tgcccgccag cagcgcagac attaaaaact gcttcattgg aatgcctgta gtccggtctg
     2341 ggcgcggccc aggatcaggg cgtggatgtc gtgggtgcct tcgtaggtgt tgaccgtttc
     2401 cagattgatg gcgtgacgca gcacgggata cagatcgctg atgccgttgg cgccgtgcat
     2461 gtcgcgggca gtgcgtgcaa tgtccagcgc cttgccgcag ttgttgcgtt tcatcaggga
     2521 aatcagttcc ggtgcgcact ggccggcgtc catcagacga cccatgcgca gggcgccgtt
     2581 caggcccagg gcaatgtcgg tttgcatgtc ggcgagtttt ttctgcacca gctggttggc
     2641 ggccagcggt ttgccgaact gggtgcgttg cagtgtgtag tcccgcgcag cgagccagca
     2701 gcattccgca gcgcccaggg tgccccaggc gatgccgtag cgcgc
//
LOCUS       DASUEG010000063         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1609321, whole genome shotgun sequence.
ACCESSION   DASUEG010000063 DASUEG010000000
VERSION     DASUEG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609321"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFX11_01030"
     CDS             complement(<1..420)
                     /locus_tag="VFX11_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937229.1"
                     /translation="MNLNLSDDQRMMRENFARFLNEESSKVRVRAAQPSGFDPRLWQG
                     LAEMGAFSMRVPEEAGGLGLGLLDATVLMEEVGRTLASGPIAETLVAARLLAQLGAEA
                     NAELLNNVMRGETVVTLAFQDIADEPLQWIAGGAVADF"
     gene            961..1872
                     /locus_tag="VFX11_01035"
     CDS             961..1872
                     /locus_tag="VFX11_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937230.1"
                     /translation="MNASKKTAVITGANAGIGYQAALKLAERDTRILMACRSMDKARQ
                     ARRDLLARVPGANIEILPLDVSELSSIKEFVARFSEQVETLDILINNAGIVTPNLSRN
                     SLGYELHLATNYLGAFALTGMLLPYFRKDAQARIVNVGSLAHRFGKFDFDDPNWNNTT
                     YNNWKAYARSKIATAGFTMELNRRLQQSGSNIIALSAHPGFAATDMGRKSGATTPKTA
                     FGRWYQGKMEAWIVAKPDRAAEPIIHAASAEGIGGGDYYGPTGLFEIKGKTGKARLNP
                     LAKNIDFGRRLWAISESLTGARYLSNL"
     gene            1912..>2412
                     /locus_tag="VFX11_01040"
     CDS             1912..>2412
                     /locus_tag="VFX11_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937231.1"
                     /translation="MKANDNIEALRQEVRAWLRNSVPDGWRDAMTNVDQDKFVALQRD
                     WFVRLSQAGYATPHWPEGWLGGGRSLAEQKVIFEEVARADAPRLILYFVALYHAACTL
                     FECGTDQQREKYLQGILKGDIWCQGFSEPNAGSDLASLRTRAERRGDRYIINGQKIWS
                     TMAQYAD"
BASE COUNT          540 a          644 c          704 g          524 t
ORIGIN      
        1 aaaatccgcc acggcgccgc cggcaatcca ttgcagcggc tcgtcggcta tgtcctgaaa
       61 cgcgagggtg acaacggttt cgccgcgcat gacattgttc agcagctcgg cattggcttc
      121 cgcacccaac tgcgccagca accgggcggc cacgagggtt tcggcaatgg ggccggacgc
      181 cagcgtcctg cccacttctt ccatcagcac agtagcgtcc agcaggccaa gccccagacc
      241 gcccgcttcc tccggaacgc gcatggaaaa ggcgcccatt tccgccagcc cctgccagag
      301 acgcggatcg aatccgctcg gttgtgctgc gcgtacgcgc accttgctgc tttcttcatt
      361 cagaaagcgc gcaaagttct cgcgcatcat gcgttggtcg tccgagagat ttaaattcat
      421 ggtgaggcga ctccctaaat gtaactgcag ggggcaagcc tgtcctgcgg acaggcaagt
      481 aaaagcgagt gactgcaata gcgattggct caaggctggc tgttgcgatc cggcttttgt
      541 tctaaggcat gcccctgttt ttgtcgttat gcccctgatt gggagctgtg gggccggctt
      601 ccatggcagg ccggctggtg cagtatctgt tggtgttacc aaagcggccg gtactaccag
      661 tcaattactg cgaaacgctt gaatatcgtt gtttttcaat gaaatccact gcggaaagcc
      721 acgtctgcgg ggcctggttt tgttgccgca aacacgacca ttttctattg aattcatgct
      781 atcacccccc acaattacta gtcaacaggg ttgtttaata atggattttg tggcacaaag
      841 gtttttgtgg atttgagtgc gaatacaacc aactgagcca gtcaataata agatcagcca
      901 gaagcggttc aggatcagcg gtatgcgaac tgcgttggcg tgtacgaagg tagaacagga
      961 atgaatgcgt caaaaaaaac cgccgtcatt acgggggcga atgcgggaat tggttatcag
     1021 gcagccttga agcttgcgga gagagacacc cggattctca tggcctgccg gagcatggac
     1081 aaggcaaggc aggcgcgccg ggatctgctt gcccgggtgc cgggtgccaa tattgagatc
     1141 ctgccgctgg atgtgtccga gctgtcatcg ataaaggaat ttgtggcgcg tttcagcgag
     1201 caggttgaaa cgctggacat attgatcaat aacgcaggga tcgtgacacc caacctgtcc
     1261 cgcaacagtc ttggctatga gttgcatctg gcgaccaatt atctgggagc ctttgcgctg
     1321 accggaatgt tgctgcccta ttttcgcaag gatgcgcaag cccgcatagt caacgttggc
     1381 agtcttgccc accgatttgg gaaatttgat ttcgacgacc ccaactggaa taacaccacc
     1441 tacaacaact ggaaagctta tgcccgcagc aaaatcgcaa cggcgggctt taccatggag
     1501 ttgaaccgtc gtctgcagca gagtggcagc aatatcatcg ccctgagtgc ccatcccgga
     1561 tttgcggcaa cggatatggg caggaagtca ggtgccacca cgccgaaaac cgcattcggc
     1621 cgctggtacc agggaaaaat ggaagcctgg atcgtggcca agcctgaccg ggcagccgaa
     1681 cccattattc atgctgccag cgcagagggt atcggtggcg gcgattacta cggtcccacc
     1741 ggcttgttcg aaatcaaagg caaaaccgga aaagcccgac tcaatccgct ggcgaagaat
     1801 atcgattttg gccggcggct ttgggctatc tccgaatccc tgacaggcgc ccgttacctt
     1861 tccaacctgt agtccgaaac gtccagaacc cggttctcaa aaggctgttg catgaaggca
     1921 aatgacaata tcgaagcgtt gcgtcaggaa gtgcgcgcgt ggttgcgaaa cagtgtgccc
     1981 gatggctggc gggacgccat gactaacgtc gatcaggaca aatttgtcgc gctgcagcgc
     2041 gactggtttg taaggttgtc gcaggccggt tatgccacgc cccactggcc tgaagggtgg
     2101 ctgggtggtg ggcgttccct ggcggaacag aaagtgattt ttgaggaagt ggcgcgtgcg
     2161 gatgcgccgc ggctgattct ctattttgtg gccctttatc atgcagcctg cacgttgttt
     2221 gagtgtggta cagaccagca gcgggaaaaa tatctgcagg ggattctgaa gggtgacatc
     2281 tggtgtcagg gattttccga gcctaatgcc ggttcggatc tggcatcgct gcgcacccgg
     2341 gcggaacgtc gcggcgaccg ttacatcatc aatggccaga aaatctggtc gaccatggcg
     2401 cagtacgccg ac
//
LOCUS       DASUEG010000064         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_483213, whole genome shotgun sequence.
ACCESSION   DASUEG010000064 DASUEG010000000
VERSION     DASUEG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483213"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="lptG"
                     /locus_tag="VFX11_01045"
     CDS             <1..887
                     /gene="lptG"
                     /locus_tag="VFX11_01045"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX4937232.1"
                     /translation="RIYDFMPVAVLIGCLVGMGTLAGTSELTVVRAAGVSIARLVYSA
                     VRPVLVIVALGLVLGQFVVPKTEQYAQSERSRNLAAGPPSSARDGYWYREEGQYIHIQ
                     AIQPNGVMYGVSRFLFDAKNDLVQSDFSERAIYQGDHWILERVRQTRMPGEHTESQTF
                     ETLRWDTSVTPDVMSILALKPDYLSITGLYNYATYLSKQSLESKNYYFSFWKKVFQPI
                     TTIVMVFIAISFVFGPLRSVTMGQRITAGVLVGLLFHYAQQLLGHASIIYDIPPLLGA
                     MLPMLICLVTGILLLRRV"
     gene            complement(895..1404)
                     /locus_tag="VFX11_01050"
     CDS             complement(895..1404)
                     /locus_tag="VFX11_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4937233.1"
                     /translation="MTTSYLDFPSAGLFKRLASMVYDLLILVAMWLLIGFLYMQLYQV
                     ITGVPANTEDPHYKQLLPFTLLPLLLAGTFTFYAWFWLHGGQTLGMRAWRLQVVDAHL
                     DGRPVNLVQCLSRFLMALISLSTFGLGYLWVLVSPSGDTWHDSLSGTRTLLLPKEENK
                     KVAPYRRRR"
     gene            1532..>2965
                     /locus_tag="VFX11_01055"
     CDS             1532..>2965
                     /locus_tag="VFX11_01055"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX4937234.1"
                     /translation="MAPQIFNRQQRAAMATPAGVRHSGQRLAGGGLAQALVLLASFLM
                     PVALLAAPKISVEILGEAVDPGIAPNVTSHVNRLGISSGAEARRLRVLIQKNAHTALE
                     AFGHYDAGIEVDIAGDNGKASITLRVTPGEPVRWRDTAVRLSGPGAQDPLFLDVLARH
                     SPQPGTPLRHDQYETLKKQLHNTALSHGYFDGEFLQQRLLIDRAGRRADVDLAFDTGV
                     RYRLGAVTFGPSELGKRELQRQVPFRPGDPYDEAQMTRLNRNLLDSGYFQSVSLQPQR
                     PKAREGEEAIVPVKVELQDAKDNRVSVGLGYGTDTGPRVKLGWVKPLLNSAGHSLNLS
                     TSLSEPKKEAVAQYKIPDGEPGYDFWLLQAGYQEEEFEDTNYELVSYGASRQQRVFGD
                     WQRTYFANVKHERGFLDGEYTEDRPSDSFYIAPGISFSHTRSEGGLHPRSGHRFSTDL
                     EFSDPWIGSDTEYVRLQVMAKYLFSLSE"
BASE COUNT          564 a          870 c          947 g          584 t
ORIGIN      
        1 ggcgtattta cgatttcatg ccggtggcgg tactgatcgg ctgtcttgtg ggcatgggca
       61 ccctggccgg taccagtgag ctgaccgtgg tgcgtgccgc cggtgtgtcg attgcgcggc
      121 tggtgtattc cgccgtgcgc ccggtgctgg tgatcgttgc gctggggttg gtgctggggc
      181 agtttgtcgt gccgaaaacg gaacagtacg cgcagagtga gcgctcgcgc aatctggcgg
      241 caggtccgcc ctccagtgcc cgggacggct attggtatcg ggaggaggga cagtacattc
      301 atatccaggc gatccagcct aacggcgtca tgtacggcgt gtcgcgcttt ctgttcgacg
      361 ctaagaacga tctggtgcag tcggattttt ccgagcgcgc gatttatcag ggggatcact
      421 ggattctgga gcgggtgcga cagacgcgca tgccgggtga gcataccgaa tcgcaaactt
      481 tcgagactct gcggtgggat acatccgtga cgccggacgt gatgtcgatt ctggcactga
      541 agccggatta cctgtcgatc acagggcttt acaactatgc cacctatctc agcaagcagt
      601 cgctggagag caagaactac tatttttcgt tctggaaaaa agtgttccag ccgatcacta
      661 ccatcgtgat ggtgttcatc gcaatatcct tcgtattcgg gccgctgcgc tcggtcacca
      721 tgggccagcg gatcaccgct ggcgtgttgg tggggctgct gtttcattac gcgcagcaac
      781 tgctggggca tgccagcatc atctacgaca ttccgccgct gctgggagcc atgctgccga
      841 tgctgatctg tctggtgacg ggtatcctgc tgctgcggcg cgtttgatct gccttcagcg
      901 ccgtctgcgg tacggcgcca ctttcttgtt ctcttctttt ggcaacagca aggtgcgggt
      961 gcccgacaga ctgtcatgcc aggtgtcgcc gctggggctg accagcaccc acagataacc
     1021 cagtccgaac gtggacagcg aaatcagcgc catcagaaag cggctcaggc actgcaccag
     1081 attgaccggg cggccgtcca gatgtgcgtc caccacctgc aggcgccagg cacgcatgcc
     1141 cagggtctgc ccgccgtgca gccagaacca ggcgtaaaaa gtgaacgtgc ccgccagcaa
     1201 tagtggcagc agcgtgaacg gcagcagctg cttgtagtgg ggatcctcgg tgttcgcggg
     1261 cacaccggtg atgacctgat acagctgcat gtacaggaag ccgatcagca gccacatggc
     1321 caccaggatg agcaggtcgt acaccattga cgccagccgt ttgaacagcc cggcagaggg
     1381 aaaatcgaga taggacgtgg tcatggtatt ccgtggacag atctgatttc gagggcgcag
     1441 cttagcataa tttccgcacc ggcccgcccg ccaaatcatg ctaaccttgc gcgattgcac
     1501 gagggatgca actgcaacgg acggagtgtt cttggcacca cagatcttca atcggcaaca
     1561 gcgtgcggcg atggccacgc cggcaggcgt ccgccactcg ggacagcgcc tggctggcgg
     1621 cgggctggcg caggcgctgg tgttgctggc gtcgttcctg atgcccgttg cgttgttggc
     1681 ggcaccgaag atcagcgtgg agatcctggg ggaggctgtt gatccgggca ttgctcccaa
     1741 cgttaccagc cacgtcaacc gtctgggtat ttccagcggt gccgaggcgc ggcggctgcg
     1801 ggtgctgatc cagaagaacg cgcacacggc gctggaagca ttcggccatt atgacgcggg
     1861 catcgaggtg gacatcgctg gcgacaacgg caaggccagc atcaccctgc gggtaacacc
     1921 gggggagccg gtgcgctggc gtgacaccgc cgtgcgtctg agtggacccg gcgcacagga
     1981 cccactgttt ctcgatgtgc tggccaggca ttcgccccaa cccggcaccc ctttgcgtca
     2041 cgatcaatac gagacgctga aaaagcagct gcacaatacc gccctgtccc acggctattt
     2101 tgatggtgag tttttgcagc aacgcctgct gatcgaccgc gcgggccgtc gcgccgatgt
     2161 ggatctggcg ttcgataccg gcgtgcggta ccggctgggc gcggtcacat tcggccccag
     2221 cgaactgggc aagcgggaac tgcagcgcca ggtgccgttc cgtcccggcg atccttacga
     2281 tgaggcgcag atgacgcgtc tgaaccgcaa cctgctcgat tccggctatt ttcaatcggt
     2341 gtcgctgcaa ccgcagcggc ccaaggccag ggagggcgaa gaggcgatcg tgccggtgaa
     2401 ggtggaactg caggatgcga aagacaaccg ggtcagcgtg ggcctgggtt atggcaccga
     2461 caccgggccc cgggtcaagc tgggctgggt caagccactg ctgaacagcg cgggccattc
     2521 cctcaatctg tccacgtcgc tgtcggaacc caagaaagag gccgtcgccc agtacaagat
     2581 tccggatggc gagccgggct acgacttctg gctgttgcag gctggttatc aggaagaaga
     2641 attcgaggac accaactacg agttggtgtc ctatggtgcc agccgccagc agcgggtgtt
     2701 tggcgactgg cagcgcacct atttcgccaa cgtgaagcac gaacgcggat ttctggacgg
     2761 cgaatacact gaagatcgtc ccagcgattc tttttacatc gcgcccggca tcagtttcag
     2821 ccacacccgc agcgagggcg ggctgcatcc ccgcagtggc caccgcttct ccaccgacct
     2881 ggaattttcc gacccctgga ttggctccga caccgaatat gtgcgcctgc aggtcatggc
     2941 gaaatacctg ttctccctga gcgag
//
LOCUS       DASUEG010000065         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_483148, whole genome shotgun sequence.
ACCESSION   DASUEG010000065 DASUEG010000000
VERSION     DASUEG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483148"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..947
                     /gene="truA"
                     /locus_tag="VFX11_01060"
     CDS             159..947
                     /gene="truA"
                     /locus_tag="VFX11_01060"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX4937235.1"
                     /translation="MRIALGVEYDGSSFGGWQRQGHDPNTVQEKLEKALSRVADHEVG
                     VFCAGRTDAGVHGVGQVVHFDTESVRDMRGWIFGCNTNLPDSISVRWAKIASDEFHAR
                     FSAKARAYRYVIYNHPVRPALGLKHLCWNFRPLELEPMQQAAQFLLGEHDFSAFRAQG
                     CQAKSPVKEVQKIDLYRRGRLIVLDIQASAFLHHMVRNIAGVLMAIGAGKYPAGWAKD
                     VLESRDRTQGAPTAAPYGLYFMRVHYPEHFEIPAADPDAPFMPL"
     gene            1047..1676
                     /locus_tag="VFX11_01065"
     CDS             1047..1676
                     /locus_tag="VFX11_01065"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007962209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX4937236.1"
                     /translation="MATRVKICGITRTEDALTAAHAGADAIGFVFYGPSPRNVAPEKA
                     AEIVRALPAFVTTVGLFVDAPVEWVEAVMHTVPLDLLQFHGDEPEPYCSRFRKPYIKA
                     IRMQDGVDLHQQARIYHSSRGLLLDTFVAGQAGGTGQTFDWSRIPRDLTKPVILAGGL
                     TPSNVAQSIAQVSPWAVDVSGGVERAKGIKDSALIDAFMQGVHSGSQRA"
     gene            1633..2874
                     /gene="trpB"
                     /locus_tag="VFX11_01070"
     CDS             1633..2874
                     /gene="trpB"
                     /locus_tag="VFX11_01070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX4937237.1"
                     /translation="MHSCKEYTVALNERDFLHTPDADGNFGPYGGRFVSETLMAALDE
                     LEEIYSRLSKDPEFQAAFDKDLAHYVGRPSPLYHAERWSKQLGGAQIYLKREDLNHTG
                     AHKVNNTIGQALLAKYTGKRRVIAETGAGQHGVATATVAARLGLECEVFMGWDDIQRQ
                     SLNVYRMKLLGAKVTPVHSGTKTLKDAMNEALRDWVTNVDHTFYIIGTVAGPHPYPML
                     VRDFQCVIGREARQQCLDQAGKLPDALIACVGGGSNAIGLFHPMLGDASVKMIGVEAG
                     GLGVSTGQHAAPLNAGRPGVLHGNRTYLMEDADGQIIPTHSVSAGLDYPGVGPEHSWL
                     KDCGRVEYVAINDDEALAAFRKLTLVEGIMPALESSHAVAYAEKLAPTMPKDSIIIVN
                     LSGRGDKDIHTVAKLDGISLG"
     gene            2896..>3303
                     /gene="trpA"
                     /locus_tag="VFX11_01075"
     CDS             2896..>3303
                     /gene="trpA"
                     /locus_tag="VFX11_01075"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX4937238.1"
                     /translation="MSRIKACFATLKENRRKALVPFITAGDPVLSATVPLLHVLAENG
                     ADLIEVGIPFSDPMADGPVIQLADERALANGVTTHKVLDMVREFRKTNTTTPIVLMGY
                     LNPVEIYGYQKFAQDAAAAGVDGMIIVDLPPEAS"
BASE COUNT          643 a          998 c         1013 g          649 t
ORIGIN      
        1 agcaggaagc acgggatctg ctgaaccggc tggaggcttg aactggccgg gttccatccc
       61 taacatggcg gtccctcaat gagaccgcgc agtaaaagag ggcttcggcc ctcttttcat
      121 tttgggcgcg gcccccgttc cagtggagaa ccttcctcat gcggattgcc ctaggcgtcg
      181 aatacgacgg cagctctttt ggcggctggc agcgccaggg ccatgatccg aatacggtgc
      241 aggaaaaact ggaaaaagcg ttgtcgcggg tggcggatca cgaggttggt gtgttctgtg
      301 ccggccgcac cgacgcgggt gtgcacgggg tggggcaggt ggtgcatttc gacaccgaat
      361 ccgtgcgcga catgcggggc tggattttcg gctgcaacac caatctgccg gacagcattt
      421 cggtgcgctg ggccaagatc gccagcgacg aattccatgc ccgcttttcc gccaaagccc
      481 gggcttaccg ttacgtcatc tacaaccacc cggtgcggcc ggcgctgggc ctgaaacacc
      541 tttgctggaa cttccgcccg ctggaactgg agcccatgca gcaggcagcg caattcctgc
      601 tgggggagca tgatttcagc gccttccgcg cccagggttg ccaggccaaa tcccccgtca
      661 aggaagtgca gaaaatcgac ctgtatcgcc ggggccgcct gatcgtgctg gacatccagg
      721 ccagcgcttt cctccatcac atggtgcgca acattgccgg cgtgctgatg gccattggtg
      781 cgggcaaata cccggcgggc tgggcgaagg acgtgctgga atcccgcgac cgcacccagg
      841 gcgcgcccac tgctgcaccc tatggcctgt atttcatgcg ggttcattat cccgagcatt
      901 ttgaaattcc ggccgccgac ccggacgccc cctttatgcc gctgtaacag cgtggtttga
      961 aagggcagca atgcgacagt ttcgccccct gcggttccct tctgaagtct gttaaactgc
     1021 ctgcccccga tcattccgag gtgactgtgg caaccagggt caaaatctgt ggcattaccc
     1081 gtactgaaga cgccctgaca gctgcccatg ccggggcgga cgccatcggc tttgtgttct
     1141 acggccccag cccccgcaat gtggcgccgg aaaaagccgc ggaaatcgtg cgcgcgctgc
     1201 ctgcgttcgt gaccacggtg ggcctgtttg tcgatgcacc ggtggaatgg gtcgaggcgg
     1261 tcatgcatac tgtgccgctg gatctgttac agttccacgg cgacgagccc gagccttatt
     1321 gcagtcgctt ccgcaagccc tacatcaagg ccattcgcat gcaggatggc gtcgatctgc
     1381 accagcaggc acgtatctac cacagcagcc gcggcttgct gctcgatacc tttgtggcag
     1441 ggcaggcggg tggtaccgga cagacgttcg actggtcacg cattccacgt gatctgacga
     1501 agccggttat tctggcgggc ggattgaccc cctccaatgt ggcccagtcc attgcgcagg
     1561 tttctccctg ggcggtggat gtcagcggcg gggtggaacg ggccaagggc attaaagatt
     1621 cagctttgat cgatgcattc atgcaaggag tacacagtgg ctctcaacga gcgtgatttt
     1681 ctgcataccc cggacgcgga cggcaatttc ggcccctatg gcggccggtt tgtttccgaa
     1741 accttgatgg ccgccctcga cgaactcgag gagatttaca gccggctgtc gaaagatccg
     1801 gaattccagg cggcgtttga caaggatctg gcgcattacg tgggccgtcc ttcccccctg
     1861 tatcacgcag agcgatggtc aaagcaactg ggcggcgcac agatctacct gaagcgggaa
     1921 gacctgaacc acaccggcgc ccataaagtg aataacacga ttggtcaggc gctgctggcg
     1981 aaatacaccg gcaagcgccg cgtgatcgcc gagactggtg ccggccagca cggcgtagcc
     2041 actgctactg ttgccgcgcg tctgggcctg gagtgcgaag tgttcatggg ctgggacgac
     2101 atccagcgcc agtcgctgaa cgtgtaccgc atgaaactgc tgggcgcgaa agtgaccccc
     2161 gtgcattccg gcaccaaaac cctgaaagac gccatgaacg aagccctgcg cgactgggtg
     2221 accaacgtcg atcacacctt ctacatcatc ggcactgttg ccggaccgca tccgtatccg
     2281 atgctggtac gcgatttcca gtgcgtcatc ggtcgtgagg cccgtcagca gtgtctggat
     2341 caggccggca aattgccgga tgcgctgatt gcctgcgtgg gcggtggttc caacgcgatt
     2401 ggcctgttcc acccgatgct gggtgatgcg tcggtcaaaa tgattggcgt ggaggcgggc
     2461 ggcctgggcg tttctaccgg tcagcatgcg gcaccgctca atgccggtcg cccgggcgta
     2521 ttgcacggca accgcaccta cctgatggaa gatgccgacg gccagatcat tcccacccat
     2581 tcagtatcgg cgggcctgga ttatcccggc gttgggcctg agcattcctg gctgaaagac
     2641 tgtggccgcg tggaatatgt ggcgatcaac gacgacgaag ccctggcagc attccgcaaa
     2701 ctgaccctgg tggaaggcat catgccggca ctggaatcca gccatgcggt ggcctatgcc
     2761 gagaagctgg cgcccaccat gcccaaggac agcatcatca tcgtcaacct gtccggccgc
     2821 ggcgataaag acattcacac cgttgccaaa ctcgatggca tttcccttgg ctaaggtgaa
     2881 caggagtttt tgaacatgag ccgcatcaag gcttgctttg ccaccctgaa agaaaaccgc
     2941 cgcaaggcac tggtgccctt tatcacggcc ggcgatccgg tactgagtgc caccgttccc
     3001 ctgctgcatg tgctggcgga aaatggcgcc gacctgatcg aagtcggcat tccgttttcc
     3061 gatccgatgg ccgatggccc ggtgatccag ctggcggacg agcgtgcgct ggcgaacggc
     3121 gtcaccaccc acaaggtgct ggacatggtg cgggaattcc gcaaaaccaa caccactacg
     3181 ccgatcgtgc tgatgggtta cctgaacccg gtggaaatct acggctacca gaagtttgcg
     3241 caggatgcag cggccgccgg cgttgatggc atgatcatcg tggatctgcc gccggaagcg
     3301 tcc
//
LOCUS       DASUEG010000066         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1808297, whole genome shotgun sequence.
ACCESSION   DASUEG010000066 DASUEG010000000
VERSION     DASUEG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808297"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1972
                     /locus_tag="VFX11_01080"
     CDS             134..1972
                     /locus_tag="VFX11_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEX4937239.1"
                     /translation="MSNRLAELEHNIGRRAANRKKPAKQDKMRSALEKMQQPRKPAPV
                     APPKPVAPAVAAQGLSGRVSRWPTRTARAMSSRAFNAISYDIPGNFWVLAQPSGMACW
                     ATVFTMMKTWRSQREMTIEQAVGTVGQKWVDIYKADTGLSGDDKPTFISTAGLVAEPP
                     QSFSIEGWENLLRSYGPVWITTDEAPGAAWAIHARIITGIYGDGSPENTKFKIVDPAG
                     GRRYEESIAVFIPKYEEEVRRTGYMRIQVVHWQSDARSEQRALSLGRSQSGRSSARAF
                     SAGFSTPPAVHSRGFDYSGALAAHQRSRALGDAIDVRYNVELVPQQTGMSCWAAGFAM
                     IVGWRDRICIDPSEIPRATGHWAQYQTGLSPEDVSVMNVWGFVPEPPQSYTVEAFADL
                     LRRYGPLWVATAEPGPHIRVITGIKGDGTPDGTKLDINDPWEQGMTSFKPSNRGAQYT
                     ETYRQFEEKQANLARQEMSVAAPVYIAHLPRLPDWMNSSTAKSFSHLVGRPITMEAPP
                     NPGNFRLLTSGVWNRNSSLNVQGGQAMWFKIRNTNVLGTTITITDQSGQLKQSIILPA
                     SSVEFVFSCFGPEPMGWRFDIRSDSDAFMVTWELWSTWVPGMPPNR"
     gene            2143..3192
                     /locus_tag="VFX11_01085"
     CDS             2143..3192
                     /locus_tag="VFX11_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4937240.1"
                     /translation="MHDQVQEYYGKTLQGSADLQTNACCTDSALPAYLKPVLANIHDE
                     VMTRYYGCGLIAPDALHGMRILDLGCGAGRDVYALSALVGEAGTVIGVDMTDEQLAVA
                     NRHRDYHADKFGFRQSNVTFLKGYIEKLDELDLPDNSIDIIVSNCVINLSPDKAAVLR
                     EAHRLLKPGGELYFSDVYSDKRVPAELAADPVLYGECLGGALYWNDFQNLAKRCGFAD
                     PRLVEDRPISISNPVIEEKIGHVRFFSATYRLFKIDGLEPACEDYGQAVVYKGTVAHQ
                     PQVFRLDKHHLIQRGKIFPVCGNTYRMLQESRFRDHFDFIGNWDTHYGIFEGCGTSLP
                     YSATAADAQGSAGCC"
BASE COUNT          723 a         1056 c          954 g          601 t
ORIGIN      
        1 tatgcaaatc ccatgagcct ggatgaattc gatctggtgg cggaactggc agaaatcggt
       61 gccgaaaaga aagtttgccc gccaccccaa tccaacaccc caaattcaac tgacacgaca
      121 gcaggtgagc accatgagca accgattggc tgaactggaa cacaacatcg gccgccgcgc
      181 ggcgaaccgg aaaaagccgg ccaagcagga caagatgcgc agcgcgctgg aaaaaatgca
      241 gcagccgcgc aaacccgcgc cggtggcacc gcccaagccc gttgcgcccg ccgtggcggc
      301 acaggggctg agtggccgtg tatcacgctg gcccacccgc acggccagag ccatgagcag
      361 ccgcgcgttc aacgccatca gttatgacat ccccggcaat ttctgggtgc tggcgcaacc
      421 ctcgggcatg gcgtgctggg ccaccgtatt caccatgatg aaaacctggc gcagccagcg
      481 ggaaatgacg atcgaacagg ccgtcggcac cgtgggccag aaatgggtgg atatctacaa
      541 ggctgacacc ggcctgtccg gcgacgacaa gcccaccttc atttctaccg ccgggctggt
      601 agccgaacca ccgcaaagct tcagcatcga aggctgggag aatctgctgc gcagctacgg
      661 ccccgtgtgg atcaccaccg atgaagcacc gggtgccgcc tgggccatcc acgcccgcat
      721 catcaccggc atttacggcg acggctcacc tgaaaacacc aaattcaaaa tagtcgatcc
      781 cgcaggtggc cgccgttacg aggaatccat cgccgtattc attcccaaat acgaggaaga
      841 ggtgcgccgc accggctaca tgcggattca ggtggtgcac tggcagagtg atgcccgtag
      901 cgaacagcgc gcactgtcgc tgggccgcag ccagtccggt cgctcatcag cacgcgcatt
      961 cagcgctggc ttcagcacac ctcccgcagt gcacagccgt ggcttcgatt attccggcgc
     1021 gcttgccgct caccagcgca gccgcgccct cggcgacgcc atcgacgtgc gctataacgt
     1081 ggaactggta ccgcaacaga ccggcatgtc ctgctgggca gcaggcttcg ccatgatcgt
     1141 cggctggcgc gaccgcatct gtatcgaccc gtcggaaatt ccccgcgcca ccggccactg
     1201 ggcgcaatac cagaccggcc tcagtccgga agatgtgtca gtgatgaacg tgtggggctt
     1261 cgtaccggaa ccgccgcaaa gctacaccgt cgaagccttt gctgatctgc tgcgccgcta
     1321 cggcccgctg tgggtggcca ctgccgagcc tggcccgcat atccgcgtga tcaccggcat
     1381 caagggggac ggcacgccag acggcaccaa actggacatc aacgatccct gggaacaggg
     1441 catgaccagc ttcaaaccat cgaatcgcgg cgcgcagtac accgaaacct atcgccagtt
     1501 cgaggaaaag caggccaacc tggcgcggca ggaaatgagc gtcgcggcgc ctgtctatat
     1561 cgcccacctg ccccggctgc cggactggat gaattcgtcc accgccaaga gcttttccca
     1621 tctggtgggc cgcccgatca ccatggaagc cccgcccaat cccggcaatt tccgcctgct
     1681 gacctccggc gtgtggaacc gaaattccag cctgaatgtg cagggcggcc aggcaatgtg
     1741 gttcaagatc cgcaatacca acgtgctggg caccaccatc accatcaccg accagagcgg
     1801 acagttgaaa cagtccatta tcctgccggc atccagtgtg gagttcgtct tcagctgctt
     1861 tggtccggaa cccatgggct ggcgctttga tatccgcagc gacagtgacg ccttcatggt
     1921 gacctgggaa ctgtggagca cctgggtacc gggcatgccg cccaaccggt gatgccagca
     1981 cagaacgctc actgaagtaa cgcaaaaaag gaaaggctcg gcagtaaacc gggcctttcc
     2041 ttttttaagt ggcgctggca gcagcgcctt gattgacaag cgcagggcgg attggcaccg
     2101 tggtcacacg cttttcattg tgcccccaat ccggagttca ccatgcacga ccaggtccag
     2161 gagtattacg gcaagaccct gcaaggttcc gccgacctgc aaaccaacgc ctgctgcaca
     2221 gacagcgccc tgcccgccta tttaaaaccg gtactggcca acatccacga cgaagtcatg
     2281 acccgctatt acggctgcgg tctgatcgcg ccggacgcgt tgcacggcat gcgcattctg
     2341 gatctgggtt gtggcgcggg tcgcgatgtc tatgcactgt ctgcgctggt gggcgaagcc
     2401 ggtaccgtga tcggcgtcga tatgactgac gaacagttgg ccgtggccaa ccgccatcgc
     2461 gactaccacg cggataaatt cgggttccgt caatccaacg tcacctttct gaaaggctac
     2521 atcgaaaaac tggatgaact ggatctgccg gacaacagca tcgacatcat cgtatccaat
     2581 tgcgtgatca acctgtcgcc tgacaaggcg gcggtattgc gggaagcgca ccgtctgctc
     2641 aagcccggcg gcgaactgta tttctccgac gtttacagcg acaaacgcgt accggcagag
     2701 ctggcggccg atccggtgct gtatggcgaa tgcctgggcg gcgcgctgta ctggaacgat
     2761 ttccagaatc tggccaagcg ctgcggcttt gccgatcctc ggctggtcga ggatcggccc
     2821 atcagcattt ccaatcccgt gatcgaagaa aaaatcggcc acgtccggtt tttcagtgcc
     2881 acctatcgtt tgttcaagat cgacggactg gaaccggcct gcgaggatta cggccaggcg
     2941 gtggtttaca aaggcaccgt tgcgcaccag ccgcaagttt tccggctcga caagcaccac
     3001 ctgatccagc ggggcaagat attccccgtg tgcggcaata cgtaccgcat gctgcaggaa
     3061 tcccgctttc gcgatcactt cgatttcatc ggcaactggg acacgcacta cggcattttt
     3121 gaaggctgcg gcaccagcct gccgtacagt gcaacggcgg ccgacgcgca aggttctgcg
     3181 ggctgctgct aaaacagttc cacgctttcc agatcgactg catccatgcc ggtcacggtg
     3241 gctggcgtgt tgttgcgttg catgaaagcc accatttcat tgacgacagc gacgaaatct
     3301 tcattcgccg ctgaatggtg gcagaaactc tttt
//
LOCUS       DASUEG010000067         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_393800, whole genome shotgun sequence.
ACCESSION   DASUEG010000067 DASUEG010000000
VERSION     DASUEG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393800"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VFX11_01090"
     CDS             complement(<1..2183)
                     /locus_tag="VFX11_01090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937241.1"
                     /translation="MQFRLNDIRSKLGRKFIVLLFLVALLPMLLFASFSYNYLGNYLY
                     RQSQQRLANETLGYSTSLYERLLLSSALFEALARGDTAGTDIDEGKRMFSSTATVPWQ
                     RWQDLSAHWPAGMRAQAQQRLEAGKLVLAARPDGSLYFLQLQKRDPQQPLFQAQLDAG
                     YLLGARATLDPDLTYCVFASFQEPLLCTQRNFLSAPEFTRLRNRHPDDGFSLEWDWGG
                     QIQLAQVRPLLVTARFGVPNWHVWVSEPRRHVFEALGELQRMFSLVVAVALLLATVLA
                     VWQIRRYLTLLMKIHEGTRAVARRDFDVAIAVDSGDELQELAESFNDMAHQLDKQFAM
                     LTGLSRIDQLILNVPDPEQVAQTTLQALQRLVSSDVMAVALCEADTSQTLAVFGYQRA
                     RNHHLLHRHELSAVEAEWFHRLGPVHRAGLNDAPYLGWLWPEHREMLGGSAYLFPIVV
                     NQRNQGLMAVAWEKSVRLPDADRELLKDFADRLAVAIAAVLREKTLYQQAHFDALTRL
                     PNRQLLKDRLEQALRHALKKRVQGAVLFVDLDKFKQVNDAGGHSLGDEILQRTAERLR
                     ICVSDEDTVARQGGDEFIVVLHEIDSPLRATRVAEKIHTMLTSPFRIGERKFFLGASV
                     GIAIFPNDGADTETLLRNADTALYRVKEEGRGHYRFFEEEMNLASQRRVMTERRIRDA
                     LEMGNIVLHYQPQWYLNRDQFSVEALVRIRDPEAGLLAPLEFIGVA"
     gene            2548..2709
                     /locus_tag="VFX11_01095"
     CDS             2548..2709
                     /locus_tag="VFX11_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937242.1"
                     /translation="MKAIIVAFIGIIFSSAAIALDLQSAAPGSDSLGVLFLLVVGIIC
                     LVIARRRSA"
BASE COUNT          539 a          917 c          794 g          522 t
ORIGIN      
        1 gccacaccga taaattccag cggcgccagc agtcccgctt ccggatcgcg gatgcgcacc
       61 agcgcctcca cgctgaactg gtcccggttc aggtaccact gcggctggta gtgcagcaca
      121 atattgccca tctccagcgc atcgcggatg cggcgttccg tcatcacccg ccgctgcgac
      181 gccaggttca tttcctcttc gaaaaaacgg taatggccgc ggccttcttc cttcacccgg
      241 tacagcgcag tgtcggcatt gcgcagcagg gtttcggtat cagcgccgtc attgggaaaa
      301 atcgcgatgc cgacgctggc gcccagaaaa aacttgcgct cgccgatacg gaagggcgag
      361 gtcagcatgg tgtggatttt ttccgccacc cgcgtggccc gcaacggcga atcgatttcg
      421 tgcagcacca cgatgaattc gtcgccgccc tgccgcgcca ccgtatcctc gtcgctgaca
      481 cagatgcgca gccgctcggc cgtgcgctgc aggatttcgt cccccagcga atgaccaccg
      541 gcatcgttca cctgtttgaa cttgtccaga tccacgaaca gcaccgcgcc ctgaacgcgc
      601 tttttcagcg cgtggcgcag cgcctgctcc agccggtcct tcagcagttg ccggttcggc
      661 agccgggtca gggcatcgaa atgggcctgc tggtacagtg ttttttcccg caataccgcc
      721 gcaatcgcca ctgccagccg gtcggcaaaa tccttcagca attcgcgatc ggcgtcgggc
      781 aggcgcacgg atttttccca ggccactgcc atcaggccct ggttgcgctg gttcactaca
      841 atgggaaaca gatacgcgct gccgccgagc atttcccggt gctccggcca cagccagccc
      901 agatagggtg cgtcattgag gccggcgcgg tgcacagggc ccagccgatg gaaccactcc
      961 gcctccaccg ccgacaactc gtggcggtgc agcagatgat ggttgcgggc acgctggtag
     1021 ccgaacacgg ccagcgtctg gctggtatcg gcttcgcaca gggcgacggc catcacatcg
     1081 ctgctcacca gccgctgcaa ggcttgcagg gtggtctgtg ccacctgctc cgggtccggc
     1141 acgttgagga tcagctggtc gatacgggaa aggcccgtca gcatggcgaa ctgcttgtcc
     1201 agctggtgcg ccatgtcgtt gaaggattcc gccagctcct gcagctcgtc tccgctgtcc
     1261 accgcgatgg ccacatcgaa atcccgccgc gccaccgccc gggtaccttc atggattttc
     1321 atcagcagcg tcaggtagcg gcggatctgc cacactgcca gcaccgtcgc cagcagcagc
     1381 gccactgcca ccaccaggga aaacattcgc tgcagctcgc ccaacgcctc gaacacgtgc
     1441 cgccgtggct cgctcaccca gacatgccag ttgggcacgc caaaacgggc cgtgacgagc
     1501 aaagggcgca cctgggccag ctggatctga ccaccccagt cccactccag gctgaaaccg
     1561 tcgtccggat ggcggttgcg caggcgggta aattccggcg ccgacaggaa attgcgctgg
     1621 gtacacaaaa gcggttcctg aaagctggcg aacacacaat aggtcagatc cggatcgagg
     1681 gtcgcccgcg cgcccagcag ataaccggca tccagttgtg cctgaaacag tggttgctgg
     1741 ggatcgcgct tttgcagctg caggaaatac aggctgccat cggggcgcgc cgccagcacc
     1801 agtttgccgg cttccagccg ttgctgggcc tgcgcgcgca ttccagccgg ccagtgcgcc
     1861 gacagatcct gccagcgctg ccagggcacc gttgcggtgg aggagaacat gcgcttcccc
     1921 tcgtcaatgt cggtaccggc ggtgtcgccc cgtgccagcg cctcgaacag ggcgctggac
     1981 agcagcagcc gctcgtacag gctggtgcta taacccagcg tttcattggc cagccgctgc
     2041 tggctctgcc ggtagagata attgcccagg tagttatagg agaaggatgc aaacaacaac
     2101 atcggcagca gcgccaccag aaacagcagc acgatgaact tgcgtcccag cttgctgcga
     2161 atgtcgttga gtctgaactg catgaggtga ggtcggccat gattccgggt tgggaaccgc
     2221 cccggcccgc agccggtggc agtctttttt cagcatagca tagcgatccg ttgcccccgt
     2281 gtcgggaact ttgacacatc ccggcaacgc gacattgcac atttgctgac caatcaatca
     2341 gtgttaattc aggccattcc gttcctgtgg attccgcccc agccggacct attccgccgc
     2401 cagcactgtc gacgctattg acaccgatca gcaaagtcat aaaaaaataa catctatata
     2461 tttgaaaata ttatacaatt tcgacatggc ccgacacttg ctttgcacag cccgccctga
     2521 acgggcccag ttatcaggag tagtaatatg aaagccatca tcgtcgcttt tattggaatc
     2581 attttcagca gcgcggccat cgcactggac ctgcagtcag ccgcccccgg cagcgatagc
     2641 ctgggcgtcc tgttcctgct ggtggtcggc atcatttgcc tggtcatcgc gcgccgccga
     2701 tccgcctgag gcaatgtcag cgaacggcgc tcgcagtaca gcgccgttcg ccaccgccct
     2761 cccgccccaa cc
//
LOCUS       DASUEG010000068         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1476473, whole genome shotgun sequence.
ACCESSION   DASUEG010000068 DASUEG010000000
VERSION     DASUEG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476473"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFX11_01100"
     CDS             complement(<1..341)
                     /locus_tag="VFX11_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937243.1"
                     /translation="MIGEPWMWGAFIVFVLVMLALDLFVFGGRKAHRVSVKEAAVWSL
                     VWVALALAFAALLWWYLKGVAGVDVAEQKSLEFLTGYVIEKSLSVDNVFVFLLIFTAF
                     AVPPEYQRRVLL"
     gene            464..1399
                     /gene="nhaR"
                     /locus_tag="VFX11_01105"
     CDS             464..1399
                     /gene="nhaR"
                     /locus_tag="VFX11_01105"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEX4937244.1"
                     /translation="MFDKTEQFAAMLNLNQLRCFWAVARAGGVHRASEQLHLTPQTLS
                     GQVSRLEDTLGVQLFERTGRRLVLTEAGRMALSYAEEIFGASRELEEILRQRPGREHP
                     RLLRVGVADAVPKAIVHSVLAPALTAGEPVRLVCREGTLTRLLADLAIHQLDMVLADA
                     PHPAALDIKCYSHLLGKSGIAFLGSSSILRNLNRKTGFPALLDSAPLLLPGPDTALRG
                     PLMRWLAAEGVRPTIIGEFDDSALMKDFGRAGAGLFPVPAALAGELCGQYSVKEVGRT
                     DALREQFFAITAERRLTDTLVEEVIATARHGLFGL"
     gene            complement(1451..2815)
                     /gene="mpl"
                     /locus_tag="VFX11_01110"
     CDS             complement(1451..2815)
                     /gene="mpl"
                     /locus_tag="VFX11_01110"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX4937245.1"
                     /translation="MHIHILGICGTFMGSLAVLAKELGYRVTGSDANVYPPMSTQLEA
                     QGIALTQGYDPAQLTPRPDLVVVGNAMSRGNPCVEYVLNEGIPYTSGPAWLAQHVLQG
                     RHVVGVAGTHGKTTTTTMVAWVLDQAGLNPGFLIGGVPNGFAASARLGGGKYFVVEAD
                     EYDSAFFDKRSKFIHYAPTTAILNNLEFDHADIFENLDAIKKQFHHLVRTVPGKGRLI
                     VPADDANLADVLAKGAWTPVERFSVDAQMPEAGWRAECLSEDGSAFAVFRDGVKQGEV
                     HWTMTGRHSVNNGMVAIMAAEHCGVSVAQACAALSSFPGVKRRMEVVGVARGVTVYDD
                     FAHHPTAIATTLGGLRKKVGDARIIAVIEPRSNTMRLGAHKDNLAPSVSDADEVIWYQ
                     PAGLNWDLGPVVAASTVPSVVLDSIDGIIAKIVTEAMPGDQVLIMSNGGFGGIHRKLV
                     EALG"
     gene            complement(2827..>3340)
                     /locus_tag="VFX11_01115"
     CDS             complement(2827..>3340)
                     /locus_tag="VFX11_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937246.1"
                     /translation="FWDICRLRRGPEDVPYSVQLLVLLVLLDFLVGVGGQLLAQPERL
                     HIALSLTALALAMDAVALWGLLAFKHLKARWVQSLATVVGIDLLLSLVALPLTLLSHV
                     VEPKSFMIGIIVVSQMLLVGWNIGLRGFVLHRALNIGMLQGNMLSLALFMLNVAVSVQ
                     IFPELVPAKG"
BASE COUNT          696 a         1109 c          970 g          565 t
ORIGIN      
        1 agcaacacgc gacgctggta ttcaggtggc acggcgaagg cggtaaagat cagcaggaag
       61 acaaagacgt tgtctaccga caacgatttc tcgatcacgt agccggtcag gaactcgagc
      121 gacttctgct cggccacgtc aacgccagcc acccccttga ggtaccacca gagcagggcg
      181 gcaaacgcca gcgccagcgc cacccagacc agcgaccaca cggccgcctc cttgacggac
      241 acgcggtgcg ccttgcgccc cccgaaaacg aacaggtcaa gcgccagcat caccaggaca
      301 aacacgatga acgcccccca catccagggt tcaccgatca tgggtacagg tgcatcagga
      361 ttcacgacaa ctctcgactt gtggcgggac acaatgcccc cgcaatggcc agcccacgct
      421 agtcccgcac acttgtcagg taaagtcgat ttaactatag aatatgttcg acaaaaccga
      481 acagtttgcc gccatgctca acctcaacca gctacgctgt ttctgggcag tcgcccgcgc
      541 cggcggcgta caccgcgcca gcgagcagtt gcacctgacg ccacaaaccc tgagcggaca
      601 ggtcagtcgc ctggaggaca ccttgggcgt acagcttttc gagcgcacgg gccggcgact
      661 ggtgctgacc gaagcaggcc ggatggccct ctcttatgcc gaggagatct tcggggccag
      721 tcgtgaactg gaggaaatac tgcggcagcg ccctggccgc gaacacccac gcctgctgcg
      781 cgtgggtgtt gccgatgccg ttccgaaggc cattgtgcat agcgtgctgg cacctgcact
      841 gactgctgga gaaccagtac gactggtctg tcgggagggc acgctgacgc gcctgctcgc
      901 tgacctggca atccaccagc tcgatatggt actggccgat gccccccacc cggccgcgct
      961 tgatatcaag tgctactcgc acctgctggg caaaagcggc attgcattcc tgggcagctc
     1021 aagcatcctg cgcaacctca accgcaagac cgggtttcct gccctgctcg acagcgcccc
     1081 cttgttactc cctggtcccg ataccgccct gcggggacca ctgatgcgct ggctggccgc
     1141 agaaggtgtc cggccgacga tcatcggcga gtttgacgat agtgcactga tgaaagactt
     1201 cggtcgcgcc ggtgcgggcc tgttccccgt gccggccgcg ctggcagggg aactgtgcgg
     1261 gcaatactcg gtaaaggaag tagggcgcac ggacgccttg cgcgagcagt tcttcgccat
     1321 cacggccgaa cgccggctga ccgatacgct ggtggaggaa gtcatagcaa ccgcccgcca
     1381 cggtctgttc ggcctctgac cgggacaggg agcgagtcat ctctgccggc cccggccaac
     1441 ccgtctccgt tcagccaagc gcctcgacca gcttgcggtg aatgccgcca aagccgccgt
     1501 tgctcatgat caatacctga tctcccggca ttgcctccgt cacgatcttc gcaatgatgc
     1561 cgtcaatcga gtccagcacg accgacggca cggtgctggc agctaccacc ggccccagat
     1621 cccagttcaa tccggcgggc tgataccaga ttacctcgtc tgcatcgctc accgacggcg
     1681 cgagattgtc cttgtgcgcg ccgaggcgca tggtgttgga acgcggctcg atcacggcaa
     1741 tgatgcgggc atcgcccacc ttcttgcgca ggccgcctag cgtggtggca atcgccgtcg
     1801 ggtgatgcgc aaaatcgtcg tacacggtca cgccgcgggc cacgcccacc acttccatgc
     1861 ggcgcttcac gccagggaag gatgacagtg ccgcacaggc ctgcgccacg ctgacaccac
     1921 aatgctcggc ggccatgatg gccaccatgc cgttgttcac gctgtggcgc ccggtcatgg
     1981 tccagtgcac ttcgccttgc ttcacgccat cacgaaacac ggcaaatgcc gaaccatctt
     2041 cgctcaggca ttcggcgcgc caaccggctt ccggcatctg cgcgtccacg gaaaaccgct
     2101 ccaccggggt ccacgcaccc ttggccagca catccgccag attggcatcg tcggccggca
     2161 cgatcaggcg gcccttcccg ggcaccgtgc gcaccagatg gtgaaactgt ttcttgatgg
     2221 catccagatt ctcgaaaatg tcggcgtggt cgaattcgag attgttgagg atggccgtgg
     2281 tcggggcgta gtggatgaat ttggagcgct tgtcgaaaaa ggccgaatcg tattcatcgg
     2341 cctcgaccac gaagtacttg ccaccgccca gacgggcgct ggccgcaaat ccgttgggca
     2401 cgccgccgat caggaagccc gggttgaggc cggcctggtc cagcacccag gccaccatgg
     2461 tggtggtcgt ggtcttgccg tgcgtccccg ccaccccaac gacatgccgt ccctgcagaa
     2521 catgctgcgc cagccaggcg ggaccggacg tgtaaggaat cccttcgttc agcacgtatt
     2581 ccacgcaggg attgccgcgc gacatggcat tgccgaccac caccagatcc gggcgcggcg
     2641 tcaactgagc cgggtcataa ccctgcgtca gcgcaatgcc ctgtgcttcc agttgagtgc
     2701 tcatgggcgg atagacattg gcatcggaac cggtgacgcg atagcccagc tccttggcca
     2761 acacggccag cgaacccatg aaggtgccgc agatgccgag aatgtgaata tgcatcaaaa
     2821 ctcctgtcag cccttggcgg gcaccagttc cggaaaaatt tgtacgctga ccgccacatt
     2881 gagcatgaac agcgccagcg acagcatgtt gccctgcaac atgccgatgt tgagcgctcg
     2941 gtgcagcaca aagccgcgca ggccgatatt ccagcccacc agcagcatct ggctgactac
     3001 gataatgccg atcatgaagg acttgggctc aacaacatgg ctgagcaagg tcagcggcag
     3061 ggccaccagg ctaagcagca ggtcgatccc gactaccgtg gccagactct gcacccatcg
     3121 ggctttcaga tgcttgaagg ccagcaatcc ccaaagcgcc actgcatcca tggcaagagc
     3181 aagcgctgtc agcgacaggg cgatatgcag ccgctccggt tgtgccagga gctggccgcc
     3241 aactccgacc agaaaatcca gcaagaccag cagcaccagc agctggacgg aataaggcac
     3301 atcttcaggc ccccgacgca atcggcaaat atcccagaac
//
LOCUS       DASUEG010000069         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1145140, whole genome shotgun sequence.
ACCESSION   DASUEG010000069 DASUEG010000000
VERSION     DASUEG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145140"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..>2106
                     /locus_tag="VFX11_01120"
     CDS             225..>2106
                     /locus_tag="VFX11_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4937247.1"
                     /translation="MKRYVITSLVLALYGHCAHADDTRVVATSDLWDMPLEELMLVPV
                     SSVATGTPTPVSRAASSVTVISRADIAAMGATDVDQVLETVPGLHITRSSQSGFARYI
                     FRGIASASNPEALVMFNGIPQKTLFSGSRSNAWAGMQVKSIERIEVIRGPGSALYGAD
                     AFAGVINIITRSGRDMEGSNAGVRAGSFDTWGAWTEQRIDFDDAVLGLVAEYEDSNGP
                     RETIHSDAQTLLDGFTGTDASLAPGPLNLGRQSTDVRMDLASGPWQARLGYQARNNVG
                     SFAGIASALDPSGLFSSERITGDIGFTQEQFRPDWDLQATLSVLHLTQVAERDSLLFP
                     PGSNPGTGVFPDGLIGNPSYWEDQARADVATAYHGFEQHILRLGSGYYWGDIYKVHET
                     KNFGPFLNPLPGGLTDVSDTPDAFLKEAQRTSGYLFLQDEWRLLDHTALTSGIRYDHY
                     SDFGDTTNPRVALVQEWRPALSTRLSYGRAFHAPTFVDLYATNNPVGLGNPALEPEVI
                     DTWELSLHHNPVAEFNYSLTGFSYRIDDAITYVGVQAANAGQRKGRGGELEGTWQPLA
                     ILRLVANYSYQVATDINTDSDVGEAPGEDAYLRLEWEFLPDWHWNTELLWVGKQLRVA
                     GDP"
BASE COUNT          398 a          727 c          607 g          374 t
ORIGIN      
        1 ggatttcttg ctggaggaat ttgtcatctt ccaatttgac tacaaactgc cacccgccgt
       61 aaagcgacgt ccatcgatgt cgaataatcc tgcccccggg caggctgcct ggggaaaagg
      121 ctgtcgaaac ttctgacctc cgcagattat tttatttctc aatcaatgcg gaacgttcgt
      181 actgcgctat attcattcgc aaatgactgg catccgggaa tcgaatgaag cgttatgtca
      241 tcaccagcct ggtactggcg ctctatggcc attgcgcgca cgcagacgac acgcgcgtgg
      301 tcgcgaccag cgacctgtgg gacatgccac tggaggagct gatgctcgtc ccggtcagca
      361 gcgtcgctac cggaacccct acccccgttt cccgggccgc ctccagcgtc accgtcatca
      421 gccgcgccga cattgccgcc atgggtgcca ccgacgtcga ccaggtgctg gaaaccgtgc
      481 ccggcctgca tatcacccgc tccagccagt ccggtttcgc ccgctacatc ttccgcggca
      541 tcgcctcggc ctccaacccc gaagccctgg tcatgttcaa cggcattccg caaaaaaccc
      601 tgttcagcgg ctcccgcagc aacgcctggg ctggcatgca ggtgaagtcg attgagcgca
      661 tcgaagtgat ccgggggccc ggctctgcgc tttacggcgc cgacgccttt gccggggtga
      721 tcaacatcat cacccgcagc gggcgcgata tggaaggcag caatgctggc gtgcgcgccg
      781 gcagcttcga cacctggggc gcctggaccg aacagcgcat cgatttcgat gacgccgtac
      841 tgggtctggt ggcggaatac gaagacagca acggcccccg ggaaaccatc cacagcgatg
      901 cccagaccct gctcgacggc tttaccggta ccgatgcgtc cctcgccccc ggcccgctca
      961 acctcggccg ccagtccacc gacgtgcgca tggacctcgc cagcggcccc tggcaggccc
     1021 gcctcggcta ccaggcgcgc aacaacgtgg gctcctttgc cggcatcgcc agtgccctcg
     1081 atccctccgg cctgttcagc agcgagcgca tcaccggcga catcggattc acgcaggaac
     1141 agttcagacc ggactgggat ctgcaggcga cgctaagcgt cctgcacctg acccaggtag
     1201 cggagcgcga cagcctcctg tttccgcctg gcagcaaccc cggcaccggc gtgtttcccg
     1261 acggcctgat tggtaacccc tcctactggg aagaccaggc ccgggccgat gttgccaccg
     1321 cctaccacgg ttttgagcag cacattctgc gcctgggcag cggctactac tggggcgaca
     1381 tctataaagt gcacgaaacc aagaacttcg gccccttcct gaacccgctg cccggtggcc
     1441 ttaccgatgt cagcgacacg ccggacgcct tcctcaagga agcgcaacgc acctccggct
     1501 acctgttcct gcaggatgaa tggcgcctgc tcgatcacac ggcacttacc agcggcatcc
     1561 ggtacgacca ctactccgac ttcggcgaca ccaccaatcc ccgtgtcgcc ctggtacagg
     1621 aatggcggcc ggcactgtcc acccgcctga gttacggccg cgcctttcac gcccccacct
     1681 ttgtcgacct gtacgccacc aacaatccgg tggggctggg gaacccggcg ctggagcccg
     1741 aagtcatcga tacctgggag ctgtccctgc accacaatcc ggttgcggag ttcaattaca
     1801 gcctgactgg cttcagctac cgcattgacg acgccattac ctatgtcggc gtgcaggcag
     1861 ccaacgccgg ccagcgcaag gggcggggcg gtgaactgga aggcacctgg cagcccctgg
     1921 ccatcctgcg cctggtggcg aactacagct atcaggtcgc caccgacatc aacaccgatt
     1981 ccgatgtggg cgaagcgccg ggggaagatg cctatctgcg gctggaatgg gaattcctgc
     2041 cggactggca ctggaacacg gaactgctgt gggtgggtaa gcagctgcgg gtggccgggg
     2101 atccgc
//
LOCUS       DASUEG010000070         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_239402, whole genome shotgun sequence.
ACCESSION   DASUEG010000070 DASUEG010000000
VERSION     DASUEG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239402"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFX11_01125"
     CDS             <1..248
                     /locus_tag="VFX11_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX4937248.1"
                     /translation="GLNIRGVPKSRPEDVAAEIIESCHHGKPEITVPRWLATIGAVEE
                     LLPEKVGTFLKKLAGAQNRLLHDTPQSRAYQDRVSRS"
     gene            379..1251
                     /locus_tag="VFX11_01130"
     CDS             379..1251
                     /locus_tag="VFX11_01130"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4937249.1"
                     /translation="MKKTVLITGCSSGIGRETALRFQREGWNVIATMRTPSSEQELVC
                     LPNVICPALDVTQETTIKQAIAEGHRTFGKIDVVINNAGYGLIGPFETFSTDQINRQF
                     QTNVLGLMSVTREILPYFRKQRSGLVINVASFAGRSIFPLYSVYHASKWAVEGFCDSL
                     QYEVSSYGIQVKIIEPGLVKTEFWGRSTDRENRTGINAYEEDARPVLDLIDVAAGLLA
                     TKPRDVAETIWKSANDDSNRLRYIVGIDAHTTLLARKVLSDRLYRGVMKAVFSRSSVS
                     MLQKISGKLQKVAT"
     gene            complement(1724..3475)
                     /locus_tag="VFX11_01135"
     CDS             complement(1724..3475)
                     /locus_tag="VFX11_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX4937250.1"
                     /translation="MSSAMRKIKVKSPLVILHGDEMAQVSFQRVLDQFVSNRLEIPLI
                     ELDLSAENRLRTNGQIVRESIEALKKYGVGIKNAGMTVNKAQLEELLAKMPEVDRSKL
                     AGLATKSPNGAIRAGIGGNITREDIPFRNLKRLTPNWIDRDIDVDTMDTGGNKHSHNE
                     ISKHTGIIKVVFVGASGNPVELHRRKINKGDPWLLASNDIEQVKSWAHNFFQRAIREK
                     RDAYLGLKDTVIPGYEGVMRDAIETIFKHEYKAAFERAGIKYFYELIDAQAARIVATP
                     PQRALWGVPENTTGRKMFKLVDALKKWGIPERHYSVSISRMSAGGGDQYGSFNMPVEE
                     DGIVKIILDGEEKCARDVRKGDPIIFMTNDRDAIKDWVGQVFRDAAKNDKEIYFGLKR
                     EYMEYDDVFSDVIKEVRQEVVNDNLQPPSFMIMRPSSQLKKMITDPPRNALYPTLNLD
                     GDIFSDISAALGGSLATASSIIESQDGTMLYEAPHGTAHDLFVKYQASGGKEAYFNSS
                     ALLYAVATALDTLGLREGNDELKAYADQLKAALIATVDQGIITADLKGKTYHPDKETI
                     VDMYGFLDAIEKNLKVV"
BASE COUNT          747 a         1015 c          952 g          867 t
ORIGIN      
        1 ctggcctgaa catccggggc gtgcccaaat caaggcccga agacgtcgcg gcagaaatca
       61 tcgagtcctg tcatcacgga aagcccgaga tcaccgttcc ccggtggttg gcgacaattg
      121 gggccgttga agaactcctg ccggaaaaag tgggaacctt cctgaagaaa ctggcgggag
      181 cacagaaccg gctgctgcat gacacgccac agtcccgcgc ctatcaagac cgtgtttccc
      241 gatcctgatg aaggcacacg aacatatcgg tattgtcccg gatgtcattc agatacctgg
      301 ttacaccgat atccggcact aatgactccc tcctgagtgc atcaacagcg atcatcagga
      361 aatgccaggc atcaaatcat gaaaaaaacg gttctgatta caggttgctc cagtggaatt
      421 ggtcgggaga ccgccctccg ttttcagcgg gaaggatgga atgtcattgc gaccatgcgc
      481 acgccttcca gcgaacagga actggtctgc ctgccaaatg tcatctgtcc agcactggat
      541 gtgacgcagg aaacgacgat caagcaagcc attgctgagg gtcataggac tttcggaaaa
      601 atcgatgtgg ttatcaacaa tgccggttat ggcctgatag gcccattcga aacgttcagt
      661 accgatcaaa ttaaccgtca gttccagacc aatgtacttg gcctgatgtc cgtaacccgc
      721 gaaatactcc cgtattttcg taaacagcgc tccggacttg tgatcaatgt agccagcttt
      781 gcaggccgtt ccattttccc tctttacagt gtctatcacg ccagcaaatg ggcagtggaa
      841 ggcttctgtg attccctgca gtacgaagtt tcctcttatg gaatacaggt caagatcatt
      901 gagccgggtc tggtaaaaac cgaattctgg ggccgctcta ccgaccgtga aaatcgcacg
      961 ggcattaatg cctatgaaga ggatgcacgt cctgtcctgg atctgattga cgttgctgcc
     1021 ggtctgcttg cgacaaaacc tcgcgatgtt gccgaaacca tctggaagtc agccaacgat
     1081 gacagcaaca ggcttcgata cattgtcgga atagatgccc atacaacgct gctggctaga
     1141 aaagtgctat ctgacaggct atatcgcggc gttatgaaag cggtattttc ccgctcgtct
     1201 gtttcgatgc tgcagaaaat ctctggaaaa ctccagaaag tggcaacata gctttttgac
     1261 agcggtattg attcggcctg cttaatgctg gcgcaatcaa tctcaaaaaa agtgggggcg
     1321 ttcaatgcgc tgcagtggcg ggaatggcac ggtctcgtcg gccactggca atttcatcgc
     1381 atgcactttt cgggacgcca ttgtctttgg tcattgaacc ggaagaacga tgattggaga
     1441 aagcttcggg cttgcccgac ggatcgaggt aaatttcagt ccaatagccc ctgttcaggt
     1501 aaagcgtcgt gtgtttgatt ctgtatccca ctcgaccaat tgcctgcacg aagatgcgtg
     1561 gaacatccgg gttactatcc tttttgattg aacacgttcg cgctgccagt caacctgctc
     1621 tctacatctt aaaggtggcg cgcaagccgg acgagatcct tttcacagtc tgggcagtag
     1681 gctcggatag atccaaagat ctgctttcag ctcattgcaa cactcacact accttcaaat
     1741 tcttctcgat cgcatccaga aagccataca tgtccacgat ggtttctttg tccggatggt
     1801 aagtcttgcc tttcaggtcc gccgtgatga tgccctgatc caccgtcgcg atcagcgccg
     1861 ccttgagctg gtcggcgtaa gctttgagct catcattacc ttcccgcaag cccagcgtat
     1921 ccagcgcggt agccaccgca tacagcagtg ccgatgaatt gaagtacgcc tccttgccgc
     1981 ccgacgcctg gtatttcaca aacagatcgt gcgcggtgcc gtggggcgct tcgtacagca
     2041 tggtgccgtc ctggctttcg atgatcgagc tggcggtggc caggctgccg cccagtgccg
     2101 cgctgatatc ggaaaaaatg tcgccgtcga gattgagcgt cggatacagc gcattgcgcg
     2161 gcgggtcggt gatcattttt ttcagctggc tggatggccg catgatcatg aacgacggcg
     2221 gctgcaggtt gtcgttcacc acttcctgcc gcacttcctt gatcacgtca ctgaacacat
     2281 catcgtattc catgtactcg cgcttgaggc cgaagtagat ttccttgtcg ttcttcgccg
     2341 catcgcggaa cacctgcccc acccagtcct tgatggcatc gcgatcattg gtcatgaaaa
     2401 tgatcggatc gcccttgcgc acgtcgcgcg cgcatttttc ttcgccgtcc aagatgatct
     2461 tgacgatgcc gtcttcttcc accggcatgt tgaaactgcc gtactggtcg ccaccgccgg
     2521 cgctcatgcg ggaaatggat acgctgtaat ggcgttcggg tatgccccat ttcttcagcg
     2581 catccaccag tttgaacatc ttgcggccgg tggtgttttc cggcacgccc cacagtgccc
     2641 gctgcggcgg agtcgccacg atccgcgccg cctgggcgtc gatcagctcg tagaagtact
     2701 tgataccagc gcgctcgaac gccgccttgt attcatgctt gaaaatggtt tcgatggcat
     2761 cgcgcatcac gccttcatag ccgggaatca ccgtgtcttt caggcccagg taggcatcgc
     2821 gtttttcgcg gatcgcgcgt tggaaaaagt tgtgggccca gctcttcacc tgctcgatat
     2881 cgttggatgc cagcagccag ggatcaccct tgttgatctt gcgccggtgc aattccaccg
     2941 gattgccgct ggcgcccacg aacaccacct tgatgatgcc ggtatgtttc gagatttcgt
     3001 tgtggctgtg cttgttgccg ccggtgtcca tggtgtccac atcgatatcg cgatcgatcc
     3061 agttgggcgt cagccgcttg agattgcgga agggaatatc ctcgcgggtg atgttgccgc
     3121 caatgccggc acggatcgcg ccgttggggg atttggtcgc caggccggcc agcttgctgc
     3181 gatccacttc cggcattttc gccagcagct cttccagctg ggccttgttc acggtcatgc
     3241 cggcattctt gatgcccacg ccatattttt tcagggcttc aatggattcg cgcacgatct
     3301 ggccgttggt gcgcagccgg ttttcggcgg acagatccag ttcgatcagc gggatctcca
     3361 ggcggttcga tacaaactgg tcgagcacac gctggaatga cacctgcgcc atctcgtccc
     3421 catgcaatat caccagcggg cttttgactt tgattttccg catcgcgctg ctcatcgtgt
     3481 tcggctccca aacgctcatt gtttccatcc gattccggtg gtgggtaatg acccgtccca
     3541 tccatcaagc ctagtgtagc cggtgccacc ccgcccgtgg c
//
LOCUS       DASUEG010000071         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_331166, whole genome shotgun sequence.
ACCESSION   DASUEG010000071 DASUEG010000000
VERSION     DASUEG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331166"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFX11_01140"
     CDS             <1..214
                     /locus_tag="VFX11_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937251.1"
                     /translation="QQAANGESAKLVVKSAFEGLDNFFVRVGMGIVSDRVIPDPQVSV
                     ELRKLIFDTQEAFAHDLDGFVQTASL"
     gene            complement(259..570)
                     /locus_tag="VFX11_01145"
     CDS             complement(259..570)
                     /locus_tag="VFX11_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4937252.1"
                     /translation="MILSERSYEEKRNFIRMKVDTMVTFTKADSKERYEGRCRNLSGA
                     GMLLETQKKLEMGDRLRVTIPSEGPEFAPLEAIVEVVRVIPQPDLHKFRLGVVIRKIC
                     N"
     gene            669..2036
                     /locus_tag="VFX11_01150"
     CDS             669..2036
                     /locus_tag="VFX11_01150"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX4937253.1"
                     /translation="MAMFSRNEFGAHAVVVGSGAVWLQKQRSRSNLKTPWFLTSQIRF
                     SIHRQKDTVRPMKPMQHNKQDGIVLIIDQEPELRGAMASWLSREGFQIHLESNGMQGL
                     EYWRIHQPAVLICDVRLPGIDGLGILSQIAEAESETQVIMMSRIEDTETVLSALRLGA
                     ADYLTKPIQDRAVLVHAVNRAFEEYHLIRQNRLYREALEAKNRELNDSLRLLKEDQEA
                     GRAVQLKMLPAPSKDYGNVHIEYKIIPSLYLSGDFVDYFSIGPRQIGFYLADVSGHGA
                     SSAFITVLLKTMANRVKQRYRESVDSVKNLLPADILRRANDELIPLGLGKHLAIFCGL
                     IDLEEKRLVFCSAAHFPPPLLVTLGQIRPLEGKGLPVGLFEDVLYENQTVELGDSFEL
                     VLFSDGILEVMPQSSVAQKEAHLVEMVGKGIHNIRRILDHLDLHRHQAVPDDIAIMTV
                     SCKGS"
     gene            2045..2593
                     /locus_tag="VFX11_01155"
     CDS             2045..2593
                     /locus_tag="VFX11_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4937254.1"
                     /translation="MAESNCGKALYAVHQGTYVVKLVGDIRVPICATFDGFIENMFHD
                     AQLNSVVIDLSEASVVDSTALGLMAKIAIQLRKRFNQKPLIISTRPDISRILSTMGFE
                     KVFNIVHEAPVKNPPMSEMPNIPCTQGSACDKVLEAHRILMEMNEKNRETFKDVVAAL
                     EYDQVDRSKDVPPVQDSKRHLM"
     gene            complement(2633..3580)
                     /gene="tal"
                     /locus_tag="VFX11_01160"
     CDS             complement(2633..3580)
                     /gene="tal"
                     /locus_tag="VFX11_01160"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018983274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX4937255.1"
                     /translation="MTNKLDQLKTMTLVVADTGDMQAIKKYKPVDATTNPSLLLKAAG
                     LPEYQPLIEAAVRETTGSGQARVADACERLAVAIGSEILNIIPGRISTEVDARLSFDT
                     AATIAKARKIINLYAKKGIGRERVLIKIAATWEGIQAARTLEQEGIHCNLTLLFSFAQ
                     AVACAEAGATLISPFVGRILDWYKASTGKKEYSKDEDPGVLSVSRIFRYYKHYGYPTI
                     VMGASFRNIGEIEALAGCDRLTISPQLMDELQQDTGHLPRALHPGQVNCTDAKLALDE
                     KTFRWLLNEDAMTTEKLAEGVRNFAKDQVLLEEMLGKRF"
BASE COUNT          762 a          968 c         1072 g          881 t
ORIGIN      
        1 ccagcaggcg gccaatggtg aaagcgccaa actggtggtg aaatcagcgt tcgaagggct
       61 ggacaacttt ttcgtgcggg tgggaatggg gattgtgagt gaccgcgtga tacctgatcc
      121 gcaagtgtca gtggaattgc gcaaactgat ttttgacacg caggaagcgt tcgcccacga
      181 cctggatggt ttcgtgcaga cggcatcgct gtaaatcagc cggtggtaca aaaaggccct
      241 cttaacaggg cctttttttc agttgcagat ttttcggatc accacgccca gccggaactt
      301 gtgcagatcc ggttgcggaa ttacccgcac gacttcaacg atcgcttcga gcggtgcgaa
      361 ttccggacct tcggatggaa tggtgacccg caggcggtcg cccatttcaa gtttcttctg
      421 ggtttcgagc agcatgccgg cgccactcag gttgcggcag cggccttcgt aacgttcctt
      481 gctgtctgcc ttggtgaagg tcaccatcgt atcgaccttc atgcgaatga aattacgttt
      541 ttcttcgtat gagcgctctg acaaaatcat gcgacctctc tgcttggcac tgctttatta
      601 ttgtcgtggg cctgtagacg aaacccgcgt ggcgcgggtt tcgattgagt atatcaaagg
      661 tccgccggat ggccatgttt tcacgcaatg aatttggtgc gcatgcggtc gttgttggct
      721 ccggcgccgt ctggttgcaa aagcagcgtt caaggagtaa tttgaagact ccttggtttt
      781 tgactagcca aatcaggttt tccattcacc gtcaaaagga caccgttcgg cccatgaagc
      841 cgatgcagca taacaagcag gatggcatcg ttctcatcat tgatcaggaa cccgagctgc
      901 gtggcgcaat ggcgtcctgg ttgagtcggg aaggttttca gatccatctt gaatccaacg
      961 gaatgcaggg gcttgaatac tggcgcattc atcagccggc cgtgctgatt tgcgatgtgc
     1021 gtctgccggg gattgatggg cttggcatac tgagccagat tgccgaggcg gaatccgaaa
     1081 cccaggtaat catgatgtcc cgcatcgagg acacggaaac cgtgctgtcc gcgttgcgtc
     1141 tgggtgctgc cgactatctg acgaagccga ttcaggatcg ggccgttttg gtgcacgcgg
     1201 tcaaccgtgc ttttgaggaa taccatctta ttcgccagaa tcgcctgtat cgggaggcat
     1261 tggaagcgaa aaaccgcgaa ctcaatgaca gtctgcgttt gctgaaggaa gatcaggaag
     1321 ccgggcgggc agtgcaactg aaaatgctgc cagcaccatc caaggattac ggcaacgtcc
     1381 acatcgaata caagatcatt ccctctctct atctcagtgg cgatttcgtc gattatttca
     1441 gtatcggccc ccgccagatc ggtttttatc tggcggacgt gtccgggcat ggtgcctcgt
     1501 cggcgttcat cacggtactg ctcaaaacca tggcgaaccg tgtcaagcag cgctatcgcg
     1561 aatccgttga cagcgtcaag aacctgcttc cggccgatat cctgcgtcgt gccaatgatg
     1621 aattgatccc cctggggctg ggcaagcacc tggcgatctt ctgcggcctg atcgacctcg
     1681 aggagaagcg gctggttttc tgcagcgccg cccattttcc accgccgctg ctggtcactc
     1741 tgggccagat ccgtcccctg gagggaaagg ggttgccggt ggggctgttt gaggatgttc
     1801 tttacgaaaa tcaaacagtt gagctgggtg acagctttga actggtgctg ttctcggacg
     1861 gtatcctgga agtcatgcca cagtcatccg tggcacaaaa agaagcgcat ctggtggaaa
     1921 tggttggaaa agggattcat aatatccggc gaattctgga ccatctggat ttgcacaggc
     1981 atcaggcggt tccagacgac attgcaatca tgacagtctc ttgcaagggc agttaagcac
     2041 catcatggct gaatccaatt gtggcaaagc gttgtatgcg gtccatcagg gcacgtatgt
     2101 cgtgaagctg gtgggtgaca tccgggttcc catttgtgcg acctttgacg gctttattga
     2161 gaacatgttc catgatgcac agctgaattc cgttgtcatc gatctgagcg aagccagcgt
     2221 ggtcgattcc actgccctgg ggttgatggc gaagattgcg atccagctgc gaaaacgctt
     2281 caaccagaaa ccgctcatca tcagcacccg ccctgatatc tcccgcatcc tgagcacaat
     2341 gggtttcgaa aaagtcttca atatcgtcca cgaagcccct gtcaagaatc ctccgatgtc
     2401 cgaaatgccc aacattccct gcacgcaggg cagcgcctgc gacaaggtgc tggaagcgca
     2461 tcgcatactg atggagatga acgaaaagaa tcgcgagacc ttcaaggatg tggtggcagc
     2521 gctggaatat gatcaggtgg acaggtcgaa agatgtgccg ccggtgcagg attccaagcg
     2581 gcacttgatg taagccggaa attctcttta tatcccctct gccagcgttc gctcagaaac
     2641 gcttgcccag catctcctcc agcaatacct gatccttcgc gaaattgcgc acgccttctg
     2701 ccagtttttc ggtggtcatg gcgtcttcgt tgagcagcca gcgaaatgtc ttttcatcca
     2761 gcgccagttt tgcatcggtg caattgacct gcccgggatg caatgcgcgt ggcagatggc
     2821 cggtgtcctg ctgcagttcg tccatcagtt gcgggctgat ggtcaggcgg tcgcagccgg
     2881 ccagcgcttc gatttcgccg atattgcgga agctggcgcc cattacgatg gtcgggtaac
     2941 cgtagtgctt gtaatagcgg aagatgcggc tcaccgacag cacgccggga tcttcatcct
     3001 tgctgtattc ctttttcccg gtactggcct tgtaccagtc caggatgcgg ccgacgaagg
     3061 gggatatcag ggtggcgccg gcctcggcgc aggccacggc ctgggcgaaa ctgaataaca
     3121 gtgtgagatt gcagtgaatg ccttcctgct ccagcgtgcg tgccgcctga atgccttccc
     3181 aggtggccgc aatcttgatc agcacccgtt cgcgaccaat gccttttttt gcgtacagat
     3241 tgatgatctt gcgtgccttg gcgatggtgg cagccgtgtc gaaggaaagg cgggcgtcca
     3301 cttcggtgga aatgcggccg ggaatgatgt taaggatttc actgccaatg gcgacggcca
     3361 gtcgttcaca ggcgtcggcg acgcgggcct gcccgctgcc cgtggtttcg cgcacggccg
     3421 cttcgatcag cggctggtat tccggcaatc cggctgcttt cagcagcagg gacgggttgg
     3481 tagtggcgtc caccggcttg tattttttga tggcctgcat gtcgccggtg tcggcaacaa
     3541 caagtgtcat ggttttcagc tggtccagct tgttggtcat gggaatcctt gcgcgttttg
     3601 ctagtggaac acaaccttat gacagatttg ggaccagact agcagcttgg cgaatcacgt
     3661 caacggtggc gtcgtcccgg tgg
//
LOCUS       DASUEG010000072         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_460448, whole genome shotgun sequence.
ACCESSION   DASUEG010000072 DASUEG010000000
VERSION     DASUEG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460448"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..935
                     /gene="gloB"
                     /locus_tag="VFX11_01165"
     CDS             150..935
                     /gene="gloB"
                     /locus_tag="VFX11_01165"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX4937256.1"
                     /translation="MSNLHIHPISAFSDNYIWCLHNQQLAVVVDPGDAGPVQRYLQQQ
                     GLQLAGILVTHHHPDHIAGINTLVEHWPGIPVWGPAQEHIPARTQALQQGNRVQLPLG
                     LELQVLDVPGHTLGHIAYFGTGASTGPVLFCGDTLFSSGCGRLFEGTPEQMFHSLGLF
                     RRLPGETRVFCTHEYTLSNLKFALAVEPDNMDIQQRKTAVEQLRADHLPSLPSTLALE
                     LKVNPFLRTDQAPVIEAVSRNCGARPASEAQTFARLRAWKDHF"
     gene            1021..2466
                     /locus_tag="VFX11_01170"
     CDS             1021..2466
                     /locus_tag="VFX11_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4937257.1"
                     /translation="MQRQVFRFASLTLAVFWIAGCALFPGREAPPATPPVAPPPPVVE
                     SVPPEPVAADELPLPPKPRKNRRITPTDSAPAEATPSAPVTVWDTFRDYAQLDLELDN
                     ARIREQRNWHIRNSRHLNQVLTQAEPYYHYVQTEAIRRGLPAELALIPIIESGYNPAA
                     RGRGPAGLWQLMPGTAKILGVRQSSWYEGRFDVVASTDAALRYLELLNRHFDGDWLLT
                     LAAYNAGEASIDNAISANRKRGLPADYWSLNLRQVTRLYIPKLLALAQLIDDPARYQF
                     NLHPIPNRPYFTAVDTQGQLDLNEAARMAGISTTELRRLNPAFRGSATDPVGPHRLLV
                     PVARADGFRQALASVPVGKRMQWDQYTIRSGDSVSTIARRFNITPAELKSINHMTSDR
                     LQAGKTLLVPHGSQRPPAVVSAKAATPATGAGAPAPAGKTRQLHKVAPGESLWVIAKQ
                     YNVGIKTLAQWNGLAPNAQLKAGHQLVIWIP"
BASE COUNT          528 a          816 c          745 g          475 t
ORIGIN      
        1 gacgatgaac gtcatcgccg tgcacctgct gaaagggctt gaagagtcgg ggcagcgtca
       61 tggtggtagg cggattcctt cattggcgat acattaaagg tgtccttcag attaacaaaa
      121 cttcgacgcc agcgagagtt ctttttgcca tgagcaacct gcacatccat cccatttctg
      181 ccttttccga caactacatc tggtgcctgc acaatcagca gcttgcggtg gtggtggacc
      241 ccggcgacgc cggcccggtg caacgctatc tgcaacaaca gggcctgcaa ctggcgggga
      301 ttctggtgac gcatcatcac ccggaccaca tcgccggtat caacacgctg gtggaacatt
      361 ggccaggcat tcccgtgtgg ggtccggcgc aggaacacat tcctgcgcgc acccaggcgt
      421 tgcagcaggg taacagggtc cagttgccac tgggtctgga gctgcaggtg ctggacgtgc
      481 cgggtcacac gctgggacac atcgcctatt tcggtaccgg cgccagcact ggcccggtcc
      541 tgttctgcgg cgatacgctg ttttcctctg gctgcggccg cctgttcgaa ggcacgccgg
      601 aacaaatgtt ccactcgctg ggcctgttcc ggcgcctgcc gggggaaaca cgggtgttct
      661 gcacccacga gtacacgctt tccaacctga aattcgccct ggcggtggag ccggacaata
      721 tggacatcca gcagcgcaag accgcagtgg agcaactgcg cgcggaccat ttgcccagcc
      781 tgccgtccac cctggcgctg gaattgaagg tgaatccttt cctgcgcacg gatcaggcgc
      841 ccgtcatcga ggcggtgagc cggaattgtg gtgcccgtcc cgccagcgaa gcacagacct
      901 ttgctagact tcgagcctgg aaggatcatt tttaaccagc agtcaaaaaa cctgacaact
      961 ctttgattac aacaagttgg gtttataaag taataaacaa cttgaactca agggtgattc
     1021 gtgcaacgtc aggtgtttcg atttgcgtcg ctgacgctgg ccgttttctg gattgcgggc
     1081 tgcgccctgt tccccggacg cgaggcgccg ccggcaaccc cgccggttgc cccgcctcca
     1141 cccgtcgttg agagcgttcc gccagaaccc gtcgctgccg acgaattgcc cctgccgcca
     1201 aagccgcgca agaaccgtcg cataaccccc accgattcag caccggccga ggccacaccc
     1261 agtgcacccg ttacggtctg ggataccttc cgcgattacg cacagctgga tctggaactg
     1321 gacaatgccc gtatccgtga gcagcgcaac tggcatatcc gcaacagccg tcatctgaac
     1381 caggtcctga ctcaggcgga accgtactac cactatgtcc agaccgaggc catccgccgc
     1441 ggcctgcccg cagaactggc gttgatcccg atcattgaga gtggatacaa ccccgccgcc
     1501 cggggccgtg gcccggcagg tctgtggcag ctgatgccgg gcacggccaa aatcctgggc
     1561 gtgcgccaga gcagctggta tgaagggcgt ttcgacgtgg tggcatccac cgacgccgcg
     1621 ttacgctatc tggaattgtt gaaccggcat ttcgatggtg actggctgct gacgctggcc
     1681 gcctataacg ccggtgaagc cagcatcgac aatgcgatca gcgccaatcg caagcgcgga
     1741 ctgcctgccg attactggtc cctgaacctg cgccaggtta cccggctgta catcccgaaa
     1801 ctgctggcac tcgcacaact gatagacgat cccgcccgct accagttcaa cctgcacccg
     1861 attccgaacc gcccctattt cacggcggtc gatacccagg gccaactgga tctaaacgaa
     1921 gctgcgcgaa tggcgggcat cagcacgacg gaactgcggc gcctgaatcc ggcctttcgc
     1981 ggcagcgcca ccgatcctgt cgggccgcac cgcctgctgg tgccggttgc ccgggccgac
     2041 ggattccgcc aggcactggc cagcgttccc gtcggtaaac gcatgcaatg ggaccagtac
     2101 acgatacgca gtggcgacag tgtcagcacc atcgcgcgtc gctttaatat cacgccggca
     2161 gaactgaaat ccatcaatca catgaccagc gaccgcctgc aggcaggcaa aacgctgctg
     2221 gttccgcacg gcagccagcg accgccagcg gttgtatcgg ccaaagccgc cacacctgcg
     2281 accggagccg gggcacccgc accggcaggc aaaacccggc agttgcacaa ggtcgctccc
     2341 ggggagtcac tgtgggtgat tgcaaagcaa tataacgtgg gaatcaaaac gctggcgcag
     2401 tggaatggtc tggcgccgaa cgcccaattg aaggcaggac atcagctggt gatctggatt
     2461 ccctgaggat gtggcaggta ccgttcagcc attcacggca gtcagtgcca gaccagcctg
     2521 ctgtgccagc atattgaaac ccgcatactg ctggtcattg aggg
//
LOCUS       DASUEG010000073         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2205491, whole genome shotgun sequence.
ACCESSION   DASUEG010000073 DASUEG010000000
VERSION     DASUEG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205491"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /gene="topA"
                     /locus_tag="VFX11_01175"
     CDS             <1..2228
                     /gene="topA"
                     /locus_tag="VFX11_01175"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX4937258.1"
                     /translation="QARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKMIVERER
                     EIRAFIPEEYWEIFADTLTGKNIDLRLQVAKQGGNEFRPTNQTDAEKALALLQKQAYK
                     VTGREDKPTKSRPAAPFTTSTLQQAASTRLGFSVKKTMMMAQRLYEAGYITYMRTDST
                     NLSQDALNSCRDYIEKKYGKRYVPENPQLYSSKEGAQEAHEAIRPTDVALLAKDVSGL
                     EQDAQKLYDLIWKRFVACQMPAAEYMSTNLSVTAGDFELRTKGRILQFDGHLIVNMPG
                     SKKSDEDIELPDVKVGETLQLKQLDHSQHFTKPPARYSEASLVKELEKRGIGRPSTYA
                     SIISTVQERGYVKLENRRFYAEKMGDIVTDRLNENFNNLMDYGFTAKMEEQLDEIAEG
                     KLNWKQVLDEFYDDFRQKLEAAGSDAKGMRANPPVETSIKCPECGRNMMIRTGSTGVF
                     LGCSGYALPPKERCKATINLIPGHESINTDDEESEARALREKHRCKKCGTAMENYLID
                     EKRKLHVCGNSPDCDGYEIEQGNFKIKGYDGPIVECDKCGSDMQLKTGRFGKYFGCTN
                     AECGNTRKLLRNGEVAPPKAEPIPMPHLRCEKTDDFFLLRDGASGLFMAASQFPKHRE
                     TRAPYVDELKSVADRIDEKYHYLLDAPETDPAGNRAIVRFSRKSKSQYVMTEQDGKPT
                     GWVATFNGMKWLAKSEAGGEKAPAGKKAAGDTVEKTGAKSAAPKKAAKKTTTKKAAAK
                     K"
BASE COUNT          565 a          677 c          685 g          364 t
ORIGIN      
        1 agcaggctcg tcgcttcctc gaccgggtgg tgggttatat ggtgtcgccg ctgctgtggg
       61 ccaagatcgc ccgtggcctg tccgccggtc gggtgcagtc cgtcgcggtg aaaatgatcg
      121 tggagcggga gcgggaaatc cgcgccttca ttcccgaaga atactgggaa attttcgccg
      181 acacgctgac cggcaaaaat atcgatctgc gcctgcaggt agcgaagcag ggtggcaatg
      241 aattccgccc caccaaccag accgatgcgg aaaaggcgct ggcgctgctg cagaagcagg
      301 cttacaaagt caccggccgc gaggacaagc ccaccaagtc ccgtcctgcc gcgccgttca
      361 ccacgtccac gctgcaacag gccgccagca cccgcctggg cttcagtgtg aagaaaacca
      421 tgatgatggc ccagcgcctg tacgaggccg gctacatcac ctacatgcgt accgactcca
      481 ccaacctgag ccaggatgcg ctgaacagct gccgcgacta tatcgagaaa aagtacggca
      541 agcgttacgt gccggaaaac ccgcagctct attccagcaa ggaaggtgcg caggaggccc
      601 acgaggcgat ccgtccaacc gatgtcgccc tgctggccaa ggatgtgagc ggcctggagc
      661 aggatgcaca aaagctctac gacctgatct ggaagcgctt cgtggcctgc cagatgccgg
      721 cggcggaata catgagtacc aacctgtcgg tcacggctgg cgatttcgag ctgcgcacca
      781 agggccgcat tctgcagttt gatggccatc tgatcgtgaa catgcctggc agcaagaaat
      841 ccgacgagga catcgaactg cctgacgtga aagtgggcga aacgctgcaa ctgaaacagc
      901 ttgaccacag ccagcacttc accaagccgc cagcgcgtta ttctgaagcc agcctggtaa
      961 aagaactgga aaagcgcggc atcggccggc cgtccactta cgcgtcgatc atttccacgg
     1021 tgcaggagcg cggctacgtc aagctggaaa accgccgctt ctacgctgag aaaatgggcg
     1081 acatcgtcac cgatcgcctg aatgaaaatt tcaacaacct gatggattac ggcttcaccg
     1141 ccaagatgga agagcagctc gacgagatcg ccgagggcaa gctcaactgg aagcaggtgc
     1201 tggacgagtt ttacgacgat ttccgccaga aactggaagc ggccggctcc gacgccaaag
     1261 gcatgcgcgc caatccgccg gtggaaacca gcatcaagtg tcccgagtgc ggccgcaaca
     1321 tgatgatccg caccggtagc accggtgtgt tcctgggctg ctccggctac gccttgccgc
     1381 cgaaagaacg ctgcaaggcc accatcaacc tgattcccgg acacgagtcc atcaatacgg
     1441 acgacgagga aagcgaagcc cgcgcgctac gggaaaagca ccgctgcaag aaatgcggca
     1501 cggcgatgga aaattacctg attgacgaaa aacgcaaact gcatgtgtgc ggcaacagcc
     1561 cggactgcga cggctacgaa atcgagcagg gcaatttcaa gatcaagggt tacgacggcc
     1621 ccatcgtgga gtgcgacaag tgcggctctg acatgcagct gaaaaccggc cgcttcggca
     1681 agtattttgg ttgcaccaat gcagagtgcg gcaacacccg caagctgctg cgcaatggtg
     1741 aggtggcgcc gcccaaggcc gagccgattc ccatgccgca cctgcgttgc gagaagaccg
     1801 acgatttctt tctgctgcgg gatggcgcct cgggcctgtt catggccgcg agccagttcc
     1861 ccaagcaccg cgagacccga gcgccttacg tggacgagct gaaatccgtg gcggacagga
     1921 tcgacgagaa ataccactac ctgctggatg cgccggaaac cgatccggcc ggcaatcgtg
     1981 ccattgtgcg tttcagccgc aagagcaaat cgcagtacgt catgacagag caggacggca
     2041 agcccacggg ctgggtggcg accttcaacg gcatgaagtg gctggcgaaa agcgaggcgg
     2101 gtggcgaaaa ggctccggcg ggcaaaaagg ccgctggtga cacggtggag aaaacgggag
     2161 caaaatctgc agcgcccaaa aaggcagcca aaaagaccac gaccaagaaa gccgctgcca
     2221 agaagtaaat ccccctgtgt gctgattcac acgcataaaa aaacggagcc ggtcatggct
     2281 ccgttttttt a
//
LOCUS       DASUEG010000074         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_791611, whole genome shotgun sequence.
ACCESSION   DASUEG010000074 DASUEG010000000
VERSION     DASUEG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791611"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFX11_01180"
     CDS             complement(<1..1150)
                     /locus_tag="VFX11_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4937259.1"
                     /translation="MARKTPIDRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWSGMGANFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQATKYGVPRIAFVNKMDRAGADFFKVCEQMRD
                     RLRATAVPIQIPIGAEENFKGVVDLVKMKAILWDEENKGTTFRYVDIPAELQEQADEW
                     HEKMVEAAAESSDVLMEKYLEGEKLTEEEIKQGLRKRTINLEIFPMLCGSAFKNKGVQ
                     AMLDAVIEYLPAPGDVPAIRGIDANSEEPVERHPVDEEPFSALAFKIMTDPYVGTLTF
                     VRVYSGVLSSGDTVYNSQNGGKERIGRILQMHANQREEIKELRAGDIGA"
     gene            complement(1181..1651)
                     /gene="rpsG"
                     /locus_tag="VFX11_01185"
     CDS             complement(1181..1651)
                     /gene="rpsG"
                     /locus_tag="VFX11_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX4937260.1"
                     /translation="MARRKIAAKRAILPDPKYGSEQLAKFVNMVMCDGKKSIAEGIVY
                     KALDEVSQKRGGDPLSVLDAALEAVRPAVEVKSRRVGGATYQVPTEVRPTRRTALAMR
                     WIIDAARARGEKSMVRRLAGELLDAADNRGTAVKKREETHRMAEANKAFSHFRW"
     gene            complement(1691..2065)
                     /gene="rpsL"
                     /locus_tag="VFX11_01190"
     CDS             complement(1691..2065)
                     /gene="rpsL"
                     /locus_tag="VFX11_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX4937261.1"
                     /translation="MATINQLVRKPRRSKAAKSDVPALEQCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVAKRRQARSKYGAKRPKS"
     gene            complement(2229..>2800)
                     /locus_tag="VFX11_01195"
     CDS             complement(2229..>2800)
                     /locus_tag="VFX11_01195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017172001.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4937262.1"
                     /translation="SSNPHDILRVLGSKELARYLVDEVQEVYRLQGVKINDKHIETII
                     RQMLRKVEVVDPGDTHFLQGEQVDRARLIEENDAALAENKLAARWDPLLLGITKASLA
                     TESFISAASFQETTRVLTDAAVRGVMDPLRGLKENVIVGRLIPAGTGLAYHTHRRELR
                     GFGMSQLEAEHAAATASVAPRPEDVFEDR"
BASE COUNT          563 a          871 c          790 g          576 t
ORIGIN      
        1 aggcgccgat gtcgccggcc cgcaattcct tgatttcttc gcgctgattg gcatgcatct
       61 gcaaaatgcg gccaatgcgc tccttgccgc cattctggga gttgtacacg gtgtcacccg
      121 aggacaggac acctgaatac acacgaacga aggtcaacgt gcccacatag ggatcggtca
      181 tgatcttgaa agcgagcgcc gaaaagggct cctcatcaac cggatgacgc tcgaccggct
      241 cttcgctgtt cgcatcgatg ccccgaatgg caggcacgtc accgggcgcc ggcaaatact
      301 cgatcacggc gtccagcatc gcctggacac ccttgttctt gaaggcagaa ccacagagca
      361 tgggaaagat ttcaaggttg atggtgcgct tgcgcagccc ctgcttgatt tcttcctcgg
      421 tcagcttttc gccttcgagg tatttctcca tcaggacgtc agaggattca gcagcagctt
      481 cgaccatctt ttcatgccac tcgtcggcct gctcctgcaa ttctgcagga atatcgacgt
      541 agcggaacgt agtgcccttg ttttcctcat cccacaggat ggccttcatc ttgaccagat
      601 caacgacacc cttgaagttc tcttcggcgc caatcgggat ctggatcggc accgcagtcg
      661 cacgcagccg atcgcgcatc tgctcgcaga ccttgaaaaa gtcggcgccg gcacgatcca
      721 tcttgttgac aaaagcgatc cggggcacgc catatttggt ggcctgacgc cataccgtct
      781 cggactgggg ctgaacccca cccaccgcgc aatacaccat gcaagcgcca tcgagcactc
      841 gcatggagcg ttcaacttcg atggtgaaat ccacgtggcc cggggtgtcg ataatgttga
      901 tgcggtgctc agggaaattc gcccccatgc cgctccagaa acaggtggtg gcagcggaag
      961 tgatggtgat cccgcgctcc tgctcctgct ccatccaatc catggtggca gcgccatcgt
     1021 ggacttcgcc aatcttgtga ctgacgccgg tatagaacag cacgcgctcc gtggtcgtgg
     1081 tcttcccggc gtcaatgtga gcggaaatac cgatattacg gtagcgatca atgggggttt
     1141 tgcgagccac ctgaaaattc cttagatccg ggacggatac ttaccagcga aaatgggaga
     1201 acgctttgtt ggcttctgcc atgcgatggg tttcttcccg ctttttgact gcggtgccgc
     1261 ggttatccgc cgcatccagc agctcgccag ccagccggcg caccatggac ttttcgccac
     1321 gcgcccgggc cgcatcaata atccagcgca tggcaagggc agtccgccgc gtcgggcgca
     1381 cttccgtcgg gacctgataa gtggccccac caacccgccg tgacttcact tcaaccgccg
     1441 gacgcaccgc ttccagcgcc gcatccagga ccgaaagggg gtcgccgcca cgcttctggc
     1501 ttacttcgtc aagcgccttg tagacgatgc cttcggcaat cgatttcttg ccgtcgcaca
     1561 tcaccatgtt gacaaacttt gccagctgct cacttccgta cttggggtcc ggaaggatgg
     1621 cccgcttggc tgcaatcttt cttcttgcca tgattcaact ccgtcgcgca agcgaccaaa
     1681 acccgaacaa tcagctcttg ggacgcttcg cgccatactt ggaacgcgcc tggcgacgct
     1741 tggcgacccc tgcggtatcc aggctgccgc gaacaatgtg ataacgcacg ccgggcaagt
     1801 ccttgacacg accgccacga atcaggacca ccgaatgctc ctgaagattg tgcccctcgc
     1861 cgccgatgta gccggtgact tcaaagccat tcgtcagacg aacgcgagcc accttgcgaa
     1921 gcgcggagtt tggcttcttg ggcgtcgtgg tatacacgcg tgtgcagaca ccgcgccgtt
     1981 gcgggcactg ctccagcgcg gggacgtcac tcttcgctgc cttgctgcga cgcggcttgc
     2041 gaaccaactg attgatcgtt gccatgaacc actcgtcgta tcggaaaaag agggtgggcg
     2101 ctcgggccgc cggacatgaa agaggcgcaa ttttagagat gcgggggtgg actgtcaacc
     2161 taaattgacg gaccgccaaa aagaaagagg gggccagcgc cccctccaag tggaattgga
     2221 tcgggatttc accgatcctc gaacacatcc tccgggcgtg gggctacgga ggcggtggca
     2281 gcggcatgtt cagcctccag ctgactcatg ccaaagccac gcagctcacg ccggtgggtg
     2341 tggtaagcca gcccggttcc ggcgggaatg agccgtccca caatgacatt ttccttgagt
     2401 ccgcgcagcg ggtccatgac gccgcggacg gcagcgtcgg tcaatacgcg ggtggtctcc
     2461 tggaaggagg ctgccgaaat gaaggactcc gtggccagag acgccttggt gataccaagc
     2521 agcaatggat cccagcgtgc cgccagcttg ttttcagcca gcgccgcatc gttctcctcg
     2581 atcaaccgcg cacgatcgac ctgctctccc tgcaggaagt gggtatcgcc cggatcaaca
     2641 acttccacct tgcgcagcat ctgccgaatg atggtctcga tgtgcttgtc gttgatcttg
     2701 accccctgca gtcgatagac ttcctgcacc tcgtccacca gataacgggc cagttccttg
     2761 gagccaagta cccgcaggat gtcgtgcggg ttggaggagc
//
LOCUS       DASUEG010000075         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1896939, whole genome shotgun sequence.
ACCESSION   DASUEG010000075 DASUEG010000000
VERSION     DASUEG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896939"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VFX11_01200"
     CDS             <1..1739
                     /locus_tag="VFX11_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX4937263.1"
                     /translation="NEWMRLTGVEKTDRILYGAPIHSAPALVNYTSRSSAGVPLEIGD
                     QTNLVFVSTNDGKLYAVDSQSGEEKLTFMPRVLLQRPAADQLSIAERMYQAARSDDPG
                     SFIYGLDSSWTVWRQDMNGDGNITAGSSNDFVYLYGGMRRGGRNYYGLDMTSAAETNA
                     SMSQMFVLEGGDTGTAFENMGQTWSEPELALIKFNGRNEVVMIVGGGYDPLYDGGRPA
                     DGVRPQGNALYFVRARAGTGRPAGEVLWWTSSALTGSGNHVTDADLTDSIPSRVKVLD
                     KDGDGFADHIYVGDLGGQLHRFDIRQSVNTEGASVTNISHDLIAQLGRRGGSSDTAAD
                     RRFFYPPSVAIMQDGTGRYAGVAIASGLQTSPKDETVDERFYFIKDREPFTSAPQGVL
                     THASTGEGSTDLELVDLQVVDDDGSALAGSPPAASDTQLNAARGYSAPLLLQAEKSMG
                     SPLIIKGTVVYTTYNREQQSGGVGDPDSGSSSAQCTGSLGSAALYSFSPEQGFISQDL
                     TRMPQTLAGNIAVLFPTDGSSDSGDDSSDLRDVQGWGGGPSGAIDLPDLDLGNIRKTR
                     WVQCPPGSAVCN"
     gene            complement(1845..2369)
                     /locus_tag="VFX11_01205"
     CDS             complement(1845..2369)
                     /locus_tag="VFX11_01205"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX4937264.1"
                     /translation="MKSITLRRLQGFTLIEAMIVLIIMAIILAFGIPAFGDLIDRTRV
                     RTATETVVEAFRVARLTAVQDRASVNVCPWNSGDTCTGNDWDKGIMVVNSKNEVIYRM
                     RFNEKLYVKKNNLSANDRVTINPSGWTPNDMASLYICAGDGDSKNAYRITISMAGKVT
                     PSEVTGSPECNNAG"
     gene            2500..3609
                     /gene="thiO"
                     /locus_tag="VFX11_01210"
     CDS             2500..3609
                     /gene="thiO"
                     /locus_tag="VFX11_01210"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX4937265.1"
                     /translation="MTTAVIVGGGVIGLLTARELLAGGMQVTLVDKADTGAEASWAGG
                     GIVSPLYPWNYVPAISALASWAQSVYPDLIQMLRQETGIDPQLNPCGLLMLDPPEAEQ
                     ALCWARQFGKNMQRVDADFVAQREPAATADFSDALWLPYVSNVRNPRLLQALKASLLR
                     HPAFTLLDNSPVLAIEQTQQGSAAVVCAERRLIADQVVVCAGAWSASLLQQAGCDLPI
                     VPVRGQMLLFDSRPGLIHSIILKDGKYLIPRLDGHILVGSTTEEVGFDKTTTDEAREL
                     LLSHAFDMVPVLRDVPVVKQWAGLRPGSPGGLPFIGRIPGWNNLHVNAGHYRNGLVLA
                     PASARLMADLLLQRAPVVDPAPYDPAGVRAVMAMV"
     gene            complement(3623..>3836)
                     /locus_tag="VFX11_01215"
     CDS             complement(3623..>3836)
                     /locus_tag="VFX11_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4937266.1"
                     /translation="RVNPAVGDRNLPDFEHGDSLEDYLTDIEKQAIMRALEETRWNRT
                     AAAKKLGMSFRSLRYRLKKLGLDTDD"
BASE COUNT          722 a         1043 c         1212 g          859 t
ORIGIN      
        1 cgaatgaatg gatgcggctg acgggtgtag aaaaaaccga ccgcattctt tacggtgccc
       61 ccattcactc tgcgccggca ctggtaaatt ataccagccg ctcgagcgca ggcgtgccgc
      121 tggaaatcgg tgatcagacc aatctggtct tcgtcagcac caacgatggc aagctctatg
      181 ccgttgattc gcagagtggt gaggaaaaac tgacattcat gccgcgagtc ttgctgcaac
      241 gtccggccgc cgaccagtta tcgatagctg aacgtatgta ccaggcggcg cgcagtgacg
      301 atccaggcag ttttatctat ggtctcgatt cctcctggac ggtctggcgg caggacatga
      361 atggcgatgg caacatcacc gcaggcagca gtaatgactt tgtttatctg tatggcggta
      421 tgcgccgggg aggccgcaat tactatggcc tggacatgac cagtgcggcg gaaacaaatg
      481 cctccatgag ccagatgttt gtgctcgaag gcggcgatac tggcactgcg tttgaaaaca
      541 tggggcaaac ctggtccgag ccggaactgg cgctgatcaa attcaatggc cgcaatgaag
      601 ttgtcatgat tgtgggtggt ggttacgatc ctctgtatga cggtggtcgt cctgcggatg
      661 gtgtgcggcc tcagggcaat gcgctctatt tcgtgcgtgc gcgggccggt actggtcggc
      721 cggctggtga ggtgctctgg tggacatcct cggcacttac cggctcgggc aatcatgtca
      781 ccgatgcgga tttgacggat tccattcctt cgcgggtcaa ggtgctcgac aaggacggtg
      841 atggatttgc agatcatatt tatgtcggcg atctgggtgg gcaattgcac cggtttgata
      901 tccgccagag tgtcaacacg gaaggcgctt cggtgaccaa tatcagtcac gatctgattg
      961 cccagctggg acggcgcggt ggcagcagtg atacagcagc tgaccgccga ttcttctatc
     1021 cgccaagtgt ggcgatcatg caggatggca ctggccgcta tgcaggcgtt gcgattgcca
     1081 gtggattgca gaccagtcct aaagacgaaa cggttgacga gcgcttttat ttcatcaagg
     1141 atcgcgagcc tttcaccagc gcgccgcaag gggtgctgac ccatgccagc acgggggaag
     1201 gcagcaccga tcttgagttg gtggatctgc aggtggtgga cgatgacggt tctgccctgg
     1261 cggggtcacc gccagcagcg tctgacacgc aactcaatgc cgcgcggggc tattccgcgc
     1321 cgttgttgct gcaggcggaa aagtccatgg gctctccgct gatcatcaag gggactgtgg
     1381 tctataccac ttataatcgc gagcagcaga gtggcggggt tggtgatccg gatagcgggt
     1441 cgtcctcagc tcagtgtacg gggtctttgg gtagtgcggc actctactcg ttttcgccgg
     1501 aacagggctt catttcgcag gacctgacgc gcatgccgca aacgctggcg ggcaatattg
     1561 cggtgctgtt cccgactgac ggtagctcgg attccggtga cgattcgtcg gatctgcggg
     1621 atgtgcaggg gtggggcggt ggaccctcgg gtgcgattga tctgccggat ctggatctcg
     1681 gcaacatccg caaaacccgc tgggttcagt gcccgcctgg ttctgcagtc tgcaattgat
     1741 tgagcggaat cagatacgga aataaaaaag gggccaagca ggcccctttt ttatttccca
     1801 gatttgtgtg ctgcggtaaa tgccgtttct ggattagagc cacctcagcc cgcgttattg
     1861 cattcaggtg agccagtcac ttcagagggt gtcaccttgc ccgccatact gatggtgatg
     1921 cggtacgcat ttttgctgtc gccatctccg gcgcaaatgt acaggctggc catgtcgttg
     1981 ggcgtccagc cactgggatt gatggtgact ctgtcgttgg ctgaaagatt gtttttcttc
     2041 acatacagct tttcattgaa ccgcatgcgg taaatcactt cattcttgct gttcaccacc
     2101 atgatcccct tgtcccagtc gttgccggtg caggtgtcgc cagaattcca gggacagacg
     2161 ttgacagatg cgcggtcctg cacggcagtg aggcgggcga cgcgaaatgc ttccacaacg
     2221 gtctcggttg cagtgcgcac ccgtgtcctg tcgatcaggt cgccaaaggc cggaatgcca
     2281 aacgccagaa tgatggccat gatgatcagc acgatcatcg cttcaatcag tgtgaatccc
     2341 tgcaaacgtc gcagcgttat acttttcatt accattcccc gtgtccattt cccaggacca
     2401 aaccggttga ttacccccac cgtggccttc agccacccca cggggtaggg taccatggct
     2461 cccccaaccc cataaaccgg cgagtggaca ggcggcgaca tgacaacagc agtaatcgtg
     2521 ggcggtggtg tcatcggttt gctgacggcg cgcgaactgc tcgccggtgg catgcaggtc
     2581 accttggtcg ataaggcgga cacaggggcc gaggcgtcct gggcgggcgg tggcatcgtg
     2641 tcgccgctgt atccatggaa ttatgtgccg gccatttccg cgctggcctc gtgggcgcaa
     2701 tcggtttacc cggacctgat tcagatgctg cgccaggaaa ccggaatcga tccccagctg
     2761 aatccctgcg gcctgctcat gctcgatccg ccggaggcgg agcaggcttt gtgttgggcg
     2821 cggcagttcg gcaagaacat gcagcgggtg gatgcggatt ttgtggcgca gcgggagcct
     2881 gccgccaccg cagacttttc tgatgcgctg tggctgccct atgtcagcaa cgtgcgcaat
     2941 ccacgactgt tgcaggcatt gaaggcgagc ctgctgcggc atccggcgtt cacgctgctg
     3001 gacaacagtc cggtactggc aatcgaacag acacagcagg gtagtgccgc ggtggtgtgc
     3061 gctgaacgtc gactgattgc ggatcaggtg gtcgtatgtg cgggtgcctg gagtgcgtca
     3121 ttgctgcagc aggctggttg cgatctgccg attgttcccg tgcgcggcca aatgctgctg
     3181 ttcgattccc gcccggggct gattcacagc attatcctga aagacggcaa gtacctgatc
     3241 ccgcgtctgg acggccatat tctggtgggc agtaccaccg aggaagtggg attcgacaag
     3301 accacgacgg acgaggcgcg tgaattgctg ctgtcacatg ccttcgacat ggtgccggtg
     3361 ttgagggatg tgccggtggt gaaacagtgg gctggcctgc gcccgggctc gcccggtggc
     3421 ctgcctttta ttggccggat tcccggctgg aacaatttgc atgtgaatgc cggacattac
     3481 cgcaacggac tggtgttggc gccggcatca gcgcgcctga tggcggacct gttgctgcag
     3541 cgcgcgccgg tggttgaccc ggcgccgtac gatccggctg gcgtccgtgc agtgatggcg
     3601 atggtgtaac cggacgaccg gctcagtcgt ccgtatccag ccccagcttt ttcagccggt
     3661 agcgcagcga gcggaaactc atgccgagtt ttttcgcggc ggcggtgcgg ttccagcggg
     3721 tttcttccag tgcacgcatg attgcctgtt tttcaatgtc ggtcagataa tcttccagcg
     3781 aatcgccgtg ctcaaagtcc ggcaggttgc ggtcgcccac ggcgggattg acgcgc
//
LOCUS       DASUEG010000076         5874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1167147, whole genome shotgun sequence.
ACCESSION   DASUEG010000076 DASUEG010000000
VERSION     DASUEG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167147"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="rpoD"
                     /locus_tag="VFX11_01220"
     CDS             complement(<1..1798)
                     /gene="rpoD"
                     /locus_tag="VFX11_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX4937267.1"
                     /translation="MTTDKEQQKSQLKQLITRGKEQGYLTYAEVNDHLPADITDPDQI
                     EDIIGMINDMGIPVHEIAPETDELLLNDNAATTDEDAAEEAAAALASVESEPGRTTDP
                     VRMYMREMGTVELLTREGEIVIAKRIEEGIRQVMHSLAYWPGCIQTILAEYERIQASE
                     EKRLSDVISGFIDPNMSDEPPVAVEVELELPEPEAKAGAEDEEEGEEGDGTSDTAEDS
                     NIDPELARANFEELAKLQQKANQAISKHGRESKKAEAALQAMADLFVTFKIVPRLFEE
                     MVRQIRGTLDTIRANERQIMNQCIRLAKMPRKNFIKSFPGNEVNLEWIDSHIKSNKPY
                     SAALENVKPEVLRAQRRILALEEQIGLSIPDIKEVNRRLSIGEAKARRAKKEMVEANL
                     RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRS
                     IADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPTPEELGVRLDMPEDKVRKVLKI
                     AKEPISMETPIGDDEDSHLGDFIEDNTIESPIESATIEGLREATRAVLAGLTAREAKV
                     LSMRFGIDMNTDHTLEEVGKQFDVTRERIRQIE"
     gene            complement(1923..3821)
                     /gene="dnaG"
                     /locus_tag="VFX11_01225"
     CDS             complement(1923..3821)
                     /gene="dnaG"
                     /locus_tag="VFX11_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX4937268.1"
                     /translation="MAGRIPDDFIDDVLTRTDLVELIDSYVPLKRTGKNYAACCPFHQ
                     EKTPSFTVSPDKQFYYCFGCGASGNAIGFLMDYTRLNFVEALEVLARNQGLEIPRDDS
                     PNQQREDHQPLFRILEQATDFYEQQLRNPKVKERPVDYLKKRGLSGQTAKHFRVGYAP
                     PGWDNLLTALGGDDKKRALLLKAGLLIENEQGRNYDRFRDRVMFPIRDNRGRVIAFGG
                     RVLGDDKPKYLNSPETPVFHKGRELYGLWEARQASNKISRFVIVEGYMDVIALAQFGI
                     TYAVATLGTATSQEHVEKLFRHSDELIFCFDGDEAGRRAAWRALENTLPTLKDGRQVK
                     FLLLPEGEDPDTLVRKDGKTYFEGLLDEATALSEFFFQHLADEVDTRTMDGRARLASL
                     AKPYLEKLPQGVFQQLMVQRLGEITQLKEIRLDPPTPANPPQQTHSPAVQHTATTGPV
                     HAEREYFGDPGPGPEHSASFPGNDDPASHPAPRPRQRQPLSRGDKSAGAKTELSLVDS
                     TIRILLNAPRQAAGTPLPDELQQLNLPRMPLLLELLQYLKQKPDASTAAILGHWQNQD
                     GGEALFNLAAREFLLPNEEQYPELEDALRRLRLQKVEQDLDQIIAGGVKDKDRFKQLL
                     HLQKELSK"
     gene            complement(3889..4335)
                     /locus_tag="VFX11_01230"
     CDS             complement(3889..4335)
                     /locus_tag="VFX11_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX4937269.1"
                     /translation="MSEIQARINEAVKDAMRAKDKDRLGTLRLATSEFKKIEVDERVT
                     LDDARVIAILDKMIKQRKDSVTQFNAGGRADLAAKEQAEISVLQEFLPAALSDAELEA
                     LMKKAIAESGAEGMKDMGKVMAILKPQVQGRTDMGAVSQKIKALLG"
     gene            complement(4440..4655)
                     /gene="rpsU"
                     /locus_tag="VFX11_01235"
     CDS             complement(4440..4655)
                     /gene="rpsU"
                     /locus_tag="VFX11_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX4937270.1"
                     /translation="MPQVKVKENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTQI
                     RKRKQAAAVKRHAKKVARDVVRRKRLF"
     gene            4879..>5874
                     /gene="tsaD"
                     /locus_tag="VFX11_01240"
     CDS             4879..>5874
                     /gene="tsaD"
                     /locus_tag="VFX11_01240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX4937271.1"
                     /translation="MRVLGIETSCDETGIAIYDSQAGILADALYSQVELHAEYGGVVP
                     ELASRDHVRKVIPLIKEVMSRAGCQPTDLDAVVYTAGPGLVGALLVGGAVAKSLAYAW
                     GVPSFPVHHMEGHLLAPMLEDPAPTAPFVALLVSGGHTMLVNVKAIGDYEILGESLDD
                     AAGEAFDKTAKMLGLGYPGGPRLAARAEQGNPQRFRFPRPMTDRPGLDFSFSGLKTFC
                     LNTRDECERSPTGLSEQDVSDICWAFQDAVVETLAIKCRRALQHTGVKQLVVAGGVSA
                     NLHLRARLQEVAGKLRAQVFYPQLKYCTDNGAMIAFAGYQRLLAGAGADLAVRARA"
BASE COUNT          964 a         1635 c         1858 g         1417 t
ORIGIN      
        1 cctcgatctg ccggatgcgc tcgcgggtca cgtcgaactg cttgccgacc tcttccagcg
       61 tgtggtcagt gttcatatcg ataccgaagc gcatgctcag caccttcgcc tcccgcgcag
      121 tcaggcccgc cagcacggcg cgggtcgctt cacgcagacc ttctatggtc gcggattcga
      181 tcggagactc gatggtgttg tcttcgatga aatcccccag atgcgaatct tcgtcgtcgc
      241 cgattggcgt ttccattgaa atgggttctt tggcgatttt cagcaccttg cgtaccttgt
      301 cttccggcat gtccaggcgt acgcccagtt cttccggcgt tggttcgcga cccatttcct
      361 gtagcatctg ccgcgacacg cggttcagct tgttgatggt ttcgatcatg tgcaccggaa
      421 tgcggatggt gcgcgcctgg tcagcaatcg agcgggtgat ggcctgtcga atccaccagg
      481 tggcgtaggt cgaaaacttg tagccgcgac gatattcgaa cttgtccact gccttcatca
      541 ggccaatgtt gccttcctga atcaggtcga ggaactgaag gccgcggttg gtgtattttt
      601 tcgcgatgga aataaccaga cgcaagttgg cttccaccat ttccttcttc gcgcggcggg
      661 cctttgcttc gccaatggac agacggcggt tcacttcctt gatgtccggg atgctcaggc
      721 cgatctgttc ttccagtgcc agaatccggc gctgcgcgcg caatacttcc ggttttacat
      781 tttccagcgc ggccgaatag ggtttgttcg acttgatatg gctgtcgatc cactccagat
      841 tcacttcgtt gccagggaaa ctcttgatga aattcttgcg cggcattttc gccaggcgaa
      901 tgcactggtt catgatctgg cgttcattgg cccggatggt gtccagtgtg ccacggatct
      961 gccgcaccat ttcttcgaac aggcgcggca cgatcttgaa ggtgacaaaa aggtctgcca
     1021 ttgcctgcag tgccgcttcg gcttttttgg attcacggcc atgcttgctg atggcctggt
     1081 tggctttctg ctgcagcttg gccagctctt cgaaattggc gcgggccagt tccggatcga
     1141 tattgctgtc ttcagcggtg tcgctggtgc cgtcgccttc ttcaccttcc tcttcgtctt
     1201 cggcaccggc cttggcttcc ggctcgggca gttcaagctc aacctcaacg gccacgggcg
     1261 gttcatccga catgttcggg tcgataaagc cactgattac gtcggaaagg cgtttttcct
     1321 cgctggcctg aatgcgttcg tattcagcca gaatagtctg gatgcagcca ggccagtagg
     1381 ccagcgagtg catgacctgg cgaatgcctt cctcgatgcg cttggcgata acgatttcgc
     1441 cttcgcgggt cagcagttcg acggtgccca tttcgcgcat gtacatgcgg acggggtcgg
     1501 tggtgcggcc gggttcgctt tcaacggagg ccagcgcagc agcagcttct tctgctgcgt
     1561 cttcgtcggt ggtggcggca ttgtcgttca gcagcagctc atcggtttcc ggtgcaatct
     1621 catgcacggg aatacccatg tcattgatca tgccgatgat gtcttcgatc tggtccgggt
     1681 cggtgatgtc ggcgggcagg tgatcattga cttccgcgta ggtcagataa ccttgttcct
     1741 tgccgcgtgt aatcaactgc ttgagctgag acttttgctg ttctttatca gtcgtcatga
     1801 ggtgcccatt tccgtccagt gcgcgaagaa cgcggcatta tacaggcatc gggatggcct
     1861 tgccataaaa tccccgggtc ccgcactggg ctttggctaa tgaattaagg tggtttattc
     1921 ggctatttgg acagttcttt ctgtaagtgt agtagctgtt tgaaacggtc tttgtctttg
     1981 actcccccgg cgattatttg atccagatcc tgctcgactt tttggagtcg cagccggcgc
     2041 agggcgtctt ccagttcggg gtattgttct tcatttggca gcaggaattc ccgcgccgcc
     2101 agattgaaca gtgcttcgcc gccgtcctgg ttttgccagt gccccaggat ggcggcggtg
     2161 gaggcgtcgg gcttctgttt caggtattgc agcagttcca gcagcagcgg catgcgcggc
     2221 aggttcagct gttgcagctc gtccggcagc ggggtgcctg cggcctggcg cggggcattc
     2281 agcaggatgc ggatggtgct gtcgaccagt gaaagttccg ttttggcgcc ggcggatttg
     2341 tcgccccggc ttaagggctg gcgctggcgc ggccgggggg cagggtgact ggcagggtcg
     2401 tcattcccgg gaaaggacgc tgaatgctcc gggcctggac ccgggtcacc aaagtattcg
     2461 cgctcggcgt ggacgggtcc tgttgttgcc gtgtgttgta cggctggcga atgagtctgt
     2521 tgtggcggat tggccggcgt gggcggatcg agccggatct ctttcagctg ggtgatttcg
     2581 cccagccgct gcaccatcaa ctgctggaac acaccttgag gcagcttttc caggtagggt
     2641 ttggccaggc tggcgaggcg ggcgcggccg tccatggtgc gggtgtccac ttcatctgcc
     2701 agatgctgga agaaaaactc cgacagggcg gtggcctcgt ccagcaggcc ttcgaaatag
     2761 gttttgccgt ccttgcgcac cagggtgtcg ggatcttcgc cttccggcag cagcaggaat
     2821 ttcacctgac ggccgtcttt cagggtgggc agggtgtttt ccagggcccg ccaggcggcg
     2881 cggcggccgg cttcgtcacc gtcgaaacag aaaatcagtt catcactgtg gcggaacagc
     2941 ttttcaacat gttcctggct ggtggcggtg ccgagggtgg ccacggcgta ggtgatgccg
     3001 aactgtgcca gggcgatcac gtccatgtag ccttccacga tcacgaaccg gctgatcttg
     3061 ttgctggcct ggcgcgcttc ccacaggccg tacaactctc ttcctttatg gaacaccggt
     3121 gtttccggcg agttcaggta tttgggtttg tcgtccccca gtacccggcc gccgaaggcg
     3181 atgacccggc cccggttgtc gcggatgggg aacatgacac ggtcacggaa gcggtcgtaa
     3241 ttgcggccct gttcgttttc gatcagcagg ccggctttca gcagcagggc gcgttttttg
     3301 tcgtcgccgc ccagggctgt cagcagattg tcccagcccg gtggtgcgta gccgacgcga
     3361 aaatgtttgg cggtctggcc gctgaggccg cgttttttca ggtagtcgac ggggcgttcc
     3421 ttcaccttgg ggttgcgcag ctgctgttcg tagaaatcgg tggcttgttc cagtatgcgg
     3481 aacaatggct ggtggtcttc ccgctgctgg ttggggctgt cgtcgcgggg gatttccagg
     3541 ccctgattgc gggccagtac ttccagcgcc tcgacaaaat tgaggcgggt gtaatccatc
     3601 aggaagccga tggcattgcc gctggcgccg cagccaaagc agtaatagaa ctgtttgtcg
     3661 ggcgacacgg taaaggacgg tgttttttcc tggtggaacg ggcagcaggc cgcgtagttc
     3721 ttcccggtgc gcttcagggg aacatagctg tcgatgagtt ccaccagatc ggtgcgggtg
     3781 agcacgtcat cgataaagtc gtcgggaatg cggcctgcca tgaaacacct tgtcgtggga
     3841 acaccgcgcg gcgggcgtcg tgcgcgcccg ccgtcatgcc ggattttctt agcctagcag
     3901 cgccttgatc ttctggctca cggcgcccat gtcggtgcgg ccctggacct gcggcttcag
     3961 gatcgccatg accttgccca tgtccttcat gccttcggcg ccggattcgg caatggcttt
     4021 cttcatcagg gcttccagtt ccgcgtcgct gagggcggcg ggcaggaatt cctgcaacac
     4081 gctgatttcc gcctgctctt tggcggccag atcggcgcgg ccgccggcgt tgaactgggt
     4141 gaccgaatcc ttgcgctgct tgatcatctt gtcgaggatc gcgatgacgc gggcgtcatc
     4201 cagtgtgacg cgctcgtcca cttcgatttt cttgaattcc gacgtggcca ggcgcagggt
     4261 gcccaggcgg tctttgtcct tggcgcgcat ggcatcttta acggcttcat ttattctggc
     4321 ttggatctct gacatgctgg gttccttcca aataagttgg cagttaggaa aacacaaata
     4381 cgccggatcg ggtagccccg ccggcgtaat cgcgtaaggt agtctggtgc ggtgtgcgct
     4441 tagaacaggc gcttgcgacg gacgacgtcg cgggccactt tcttggcgtg gcgcttcact
     4501 gcggcagctt gcttgcgctt gcggatctgg gtgggctttt cgtaaaactc gcgacggcga
     4561 acttctgcca gaacacccgc tttttcgcag gaacgcttga aacgacgcag tgcaacgtcg
     4621 aacggctcgt tttctttgac cttgacctga ggcatttaac tcatcacctt actgtatgca
     4681 tgctgtggca aattcactta tggtcacgcc gggcgcgggc cataaaacct gattcgatta
     4741 ccgattctgt gtggtcggga tacagcatgt ttagtcctgg accacagggg tggtgcattt
     4801 taaagggatt gcccggcaat tgcaaagcct cctgattccg ttaatatcgc gccctttcca
     4861 gccacaaggt gaacgttcgt gcgggtattg ggaatcgaaa cctcctgcga tgaaaccggc
     4921 atcgccattt atgacagcca ggccgggata ctggcggatg cgctgtacag ccaggtggaa
     4981 ctgcacgctg aatacggcgg ggtggtgccg gaactggcgt cgcgcgacca tgtgcgcaag
     5041 gtcattcccc ttataaagga agtgatgtcc cgggctggct gccagccgac ggacctggat
     5101 gcggtggttt ataccgctgg ccccggtctg gtgggggctt tgctggtggg gggcgcggtg
     5161 gcgaaaagtc tggcctacgc ctggggtgtg cccagctttc ccgtgcacca tatggaaggt
     5221 catctgctgg cgcccatgct ggaagacccg gctccgacgg cgccgttcgt cgctctgctg
     5281 gtgagtggcg gccacaccat gctggtgaat gtaaaggcta ttggcgacta cgaaatcctg
     5341 ggggaatccc ttgatgacgc tgccggtgaa gccttcgaca agaccgccaa gatgctgggg
     5401 ttgggttatc ccggcggccc ccggctggcg gcccgggcgg aacagggcaa tccgcagcgc
     5461 ttccgtttcc cccgcccgat gaccgatcgc cccggcctgg acttcagctt cagcggcctg
     5521 aaaacctttt gtctcaatac tcgtgacgaa tgtgagcgct cgccaactgg cttgagcgaa
     5581 caggatgtca gtgacatctg ctgggcgttt caggatgccg tggtggagac gctggcgatc
     5641 aagtgccggc gggcgctgca acacaccggt gtgaaacagc tggtggtggc cggtggcgtc
     5701 agtgccaacc tgcatctgcg ggcgcggttg caggaagtgg ctggcaagct gcgggcccag
     5761 gtgttttatc cccagctgaa atactgcacc gataacgggg cgatgatcgc gtttgccggg
     5821 tatcagcgtc tgctggcggg agccggggcg gatctggcgg tacgggcgcg ggcg
//
LOCUS       DASUEG010000077         6408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_18670, whole genome shotgun sequence.
ACCESSION   DASUEG010000077 DASUEG010000000
VERSION     DASUEG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18670"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX11_01245"
     CDS             <1..377
                     /locus_tag="VFX11_01245"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4937272.1"
                     /translation="ESNRTNVQPATLLEDERFGVNHTQVGHEVALQWFLPESIAEAIL
                     YHHQDLATQDEEDQQNDQVYSLIGILKMAEFISATLRKAWRNDDSHEWNVIGPRVLQV
                     MGINEDDFTEVCGEVMEDFRGS"
     gene            429..1151
                     /locus_tag="VFX11_01250"
     CDS             429..1151
                     /locus_tag="VFX11_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013784131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HEX4937273.1"
                     /translation="MNHPNSILNLRQQTLAQSTRTFRARGGKVVRCPDCIMPQGDCIC
                     ADRPRVTSTSAFCFLLYQGEAYKPSNTGRLICDVVADNHAFLWDRTRPDPALLALLKD
                     PRYAPVVVFPHEYAEAERCIAHPRQVPAVLDGKKPLFVMLDGTWREAKKMFRSPYLAT
                     LPVLGIQPQQASAYLMRDAALSHQLCTAEVGIAVLRLLQDTNAADALADYFALFCRRY
                     TVVRPHLLEKVRATNGLPVPQG"
     gene            complement(1123..2436)
                     /locus_tag="VFX11_01255"
     CDS             complement(1123..2436)
                     /locus_tag="VFX11_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937274.1"
                     /translation="MHYFRIFCLLLWSWAGTALASSLPPLSENEAYKPILVEGEDLPM
                     ALGVPLEELSLAAIVDDVMEPVPFQIDQYSEGGAPWFDGWEIPLAGASGRLNPTDKLL
                     FIYKDAGPRRVSDVPVDGTVLADITLTDSDGVERHVYLLRHSRLRSEEQYVRYSAELG
                     QVETDFYSLRYQGNNHLRWDDLQFAGFVGERPLDSMKLKVSGGVMLPQLRMDLTNDNM
                     VATPAGAIIGPIRTTTQADFNVHFMGVHVVRFSLQIHHYPKSVIYDVRGVMPGMLRQF
                     AAEPTVSMEVDANSMLGAQVRTATGPQQPATVDGKMGELESAMLQAGMNREQNWVWVS
                     SKRNLDILAFLDYAGDFNEPFSLLLQDDLHMQEDGEMFPGRLPSVGYRIDHLPEGGSI
                     GILASIYFSADFGGEPGQVAQQLRTQPEMRVIPVQNAIPGELANR"
     gene            complement(2580..3749)
                     /locus_tag="VFX11_01260"
     CDS             complement(2580..3749)
                     /locus_tag="VFX11_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008842491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEX4937275.1"
                     /translation="MRKLPRDKDNDYSRELASARCETVRAETGAALTHVSSYSFDPAV
                     LPGNIENFSGVAQIPLGFAGPMLVNGEFAQGEFYVPMATTEGTLVASYSRGMRLTREA
                     GGIRTTVIKDSMQRAPVFVFSNARAARDFGDWVTHNFAQIKAKAEETTRSGKLIEIEQ
                     HASAKMRWLRFNYSCGDAAGQNMVSKATRHACMWMLDQGIPGLEHFSLAANFDTDKKH
                     SFINSLNTRGKRVIAEVTLPADLIKNVMHTTAEKLHQQRQLSNMGAIMCGSVNNGAHF
                     ANGITAMFIACGQDVANVAESHAGFVYSELKDNGDYYFAVTIPSLVVGTYGGGTGLPT
                     QKECLDVLGCYGKDKVKKFAEIVAATVLCGELSLASAIVSDQWVSSHDQLGRNRP"
     gene            complement(3841..>6408)
                     /locus_tag="VFX11_01265"
     CDS             complement(3841..>6408)
                     /locus_tag="VFX11_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937276.1"
                     /translation="NGGASVMPYNRFFIFMNAPTDGELLDNGDWIEQVFTHEYVHILH
                     LDQADGLPSGLRNVFGRIFFNFPQAFSPLWITEGIAVKGETDAQKGFGRGQSALYDAM
                     MRAEYSKGFRTYSQTSYQGYWGTDWPSGQVYLYGYYFFEFLEQRHGHEKAMAYLKNWN
                     SNLIPWRMNSRAQDTFNISAEALWQQYIDYLNEKFSAQVKQIPAGEQTAVVSDGRINT
                     NPEWLPGGDFYYYRNDGRNKPSLQRIDAQGVETRVAKVDRFVQFDVHPRAGVLLARED
                     VCDNRKVYTDLYRLKDNGRWRRITHCGRYPSIAWSTSGEQIAAVHVQGGQNQIALLDG
                     EGELQQLLKALPAGDTIGQIAWSPDDRQLVAAIRRRASGWNLELLDLATGNWRPLTAN
                     SDLEQQPRFSADGKSVYFISDQNHLWNVRRLDMASGRVETVSHTQTAIVDYALDETTA
                     QMRVAEYTADGVMLRQQKILPQQTVYAAVTAQPAALPTIVNQTGFNPDQYSDSTDYSA
                     LSTIAPHGWFALLYADTQDNTALQFLVNGQDVLGYHFWQLAPTFYLDKDKVGGSAAYI
                     AFHRLALLWDSTVDVEVEAEPGVLEQWDTETRYQAVWMQPFNSFDGTFRIDTGVGTEN
                     VVREMENYGEIADFNDNFGGIALSWADYDNYLHSISVEDGRWIKLNYEKYNLLGGAYH
                     EGEAKTLDWREYISLINNHVLAIRAVAGRAGDDTKPYELGNELDQFESMGGNIGFGKT
                     GYTLRGYNDGYDELSGTNLRLYSAEWRFPLLEHFDGFTTFPLGIGKAAMHLFVDHGAA
                     WDKSEGHHYYTGAGFEIKPDLLVGYSTFKLDSTIGFAKGLDEDIGETTVYLRLGASF"
BASE COUNT         1271 a         1951 c         1870 g         1316 t
ORIGIN      
        1 tcgaatccaa tcggaccaat gtccagcccg ccaccttgct ggaagacgag cgcttcggtg
       61 tgaaccacac ccaggtgggg cacgaggtgg cactgcagtg gttcctgccg gagagcattg
      121 ccgaagccat cctgtatcac caccaggatc tggcgacgca ggacgaggag gatcagcaga
      181 acgatcaggt gtattccctg atcggcattc tgaaaatggc ggaattcatc agcgccacgc
      241 tgcgcaaggc ctggcgcaat gacgacagcc acgaatggaa tgtgattggt ccgcgcgtgt
      301 tgcaggtaat gggcatcaat gaagatgatt tcactgaagt ctgcggtgag gtcatggagg
      361 attttcgcgg tagctgatgg ccgcagggct ggtatcatac ccatcccttc attgtggcga
      421 cggcaactgt gaaccacccc aactccattc tgaatctgcg ccagcagact ctggcccaat
      481 ccacccgcac gtttcgcgcc cggggcggca aggtcgttcg ctgcccggac tgcatcatgc
      541 cgcagggcga ctgcatttgc gccgaccgcc cgcgggtcac cagcaccagc gcattctgct
      601 tcctgctgta ccagggtgaa gcctacaagc ccagcaacac cggccgcctg atctgcgatg
      661 tggtggcaga caatcacgcc tttctgtggg atcgcacccg gcccgatccg gccctgctgg
      721 cactgctgaa ggatccccgt tatgcgcccg tggtggtgtt tccccatgaa tatgccgaag
      781 cggagcgctg tatcgcgcat ccgcggcagg tgccggcggt gctcgacggg aaaaagccgc
      841 tgtttgtgat gctggacggc acctggcgcg aggcgaagaa aatgtttcgc agcccctatc
      901 tggcgacgct gccggtgctg ggcatccagc cgcagcaggc gtcggcgtat ctgatgcggg
      961 atgccgcgct gtcacaccag ttatgcacgg ccgaagtggg cattgccgta ctgcgattgc
     1021 tgcaggacac caatgcggcg gatgcgctgg ccgactactt cgcgctgttt tgccggcgct
     1081 ataccgtggt gcggccgcat ctgctggaga aggtgcgcgc gactaacggt ttgccagttc
     1141 cccagggata gcgttctgaa ccggaatcac ccgcatctcc ggctgggtgc gcagctgctg
     1201 cgcgacctgg ccgggttcac caccgaaatc cgcgctgaaa taaatactcg ccagaatccc
     1261 gatggacccg ccttccggca agtgatcgat acgataaccc acactgggca gccggcccgg
     1321 gaacatctcg ccgtcttcct gcatgtgcag atcatcctgc aacagcagtg agaacggctc
     1381 attgaaatcg ccggcgtaat ccagaaacgc cagaatatcc agattgcgct tgctgctgac
     1441 ccagacccag ttctgctccc ggttcatgcc tgcctgcagc atcgcgctct ccagctcgcc
     1501 catcttgcca tcgacggtcg ccggctgttg cggtccggtg gccgtgcgca cctgggcgcc
     1561 cagcatgctg ttggcatcca cttccatgct taccgtaggt tccgctgcaa actggcgcag
     1621 catgccgggc atcacgccgc gcacgtcgta aatcaccgac ttgggataat ggtgaatctg
     1681 cagactgaaa cgcaccacat gcacgcccat gaaatgcacg ttgaaatctg cctgggtggt
     1741 ggtgcggatc gggccgatga tcgccccggc tggcgtcgcc accatgttgt cgttggtgag
     1801 gtccatgcgc agctgcggca gcatcacgcc accactgact ttcagcttca tgctgtccag
     1861 cggccgttcg ccgacaaagc ccgcgaactg cagatcgtcc cagcgcaggt gattgttgcc
     1921 ctggtaacgc agggaataga aatccgtttc cacctggccc agctccgcgc tgtaacgtac
     1981 gtactgctcc tcggaacgca ggcgggaatg gcgcagcagg taaacgtggc gctcgacgcc
     2041 gtcgctgtcc gtcagggtga tgtccgccag cacggtgcca tccaccggga cgtcggacac
     2101 acggcgcggc cctgcatcct tgtagatgaa cagcagcttg tcggtaggat tgagtcggcc
     2161 ggacgcaccg gccagcggaa tttcccagcc atcgaaccag ggcgcaccgc cctcgctgta
     2221 ctggtcgatc tggaacggca ccggctccat gacatcatcg acaatggccg ccagcgacaa
     2281 ttcctccagc ggcacgccaa gggccatggg caggtcctcg ccttccacca gaatgggttt
     2341 gtaggcttcg ttttcagaaa gcggtggcaa ggaggaggcg agcgcggtac cggcccagct
     2401 ccacagtaac aggcagaaaa ttcggaagta gtgcatatcc cgggcgtccc ggccgcataa
     2461 cggtcatttg ttgttgtttt gatgacagca agacaaccgg gatgctagca cgaccaaatg
     2521 gtcaccgaat tggcagaaaa cgacagggcc tgacgcgcga atcggcaagc cccggagact
     2581 cagggccggt tgcgccccag ctgatcgtgg ctggacaccc actgatccga cacgatggca
     2641 gaggccagcg acagctcgcc gcacagcacg gtggccgcga cgatctcggc gaatttcttc
     2701 accttgtcct tgccgtagca gcccagcaca tccaggcatt ccttctgcgt cggcaggccg
     2761 gtgccaccgc cgtaggtacc caccaccaat gagggaatcg tcaccgcgaa atagtaatcg
     2821 ccgttgtcct tcaattcgct gtagacgaag ccggcatggg attccgccac gttcgccacg
     2881 tcctggccac aggcgatgaa catcgcggta atgccgttgg cgaagtgcgc gccattgttc
     2941 accgagccgc acatgatcgc acccatgttc gacagctggc gttgctggtg caatttttcc
     3001 gccgtggtgt gcatgacgtt tttgatcagg tccgccggca aggtgacttc cgcaatcacg
     3061 cgtttgccgc gcgtgttaag gctgttgatg aaggaatgct ttttgtcggt gtcgaaattc
     3121 gccgccaggc tgaaatgctc caggccggga atgccctgat ccagcatcca catgcaggcg
     3181 tgacgggtgg ccttgctcac catgttctgc ccggccgcat caccgcaact gtaattgaag
     3241 cgcagccagc gcattttcgc cgatgcatgc tgttcgattt cgatcagctt gccactgcgg
     3301 gtggtttcct ctgccttcgc ttttatctgt gcgaaattgt gcgtcaccca gtcgccgaaa
     3361 tcccgcgcgg cccgcgcgtt gctgaacacg aacaccggtg cgcgctgcat ggaatccttg
     3421 atcacggtgg tgcgaatacc acccgcttcc cgcgtcaatc gcatgccgcg actgtaactt
     3481 gccaccaagg tgccttcggt agttgccatg ggcacataaa attcgccttg cgcaaattca
     3541 ccgttcacca gcatcgggcc agcaaatccc aacggaatct gcgccacacc ggaaaaattt
     3601 tcgatattgc caggcagcac ggccggatcg aaggagtagg aactcacatg ggtcagagca
     3661 gcaccggttt cggcgcgcac agtttcgcag cgggcactgg ccagttcacg gctgtaatcg
     3721 ttgtctttgt cgcggggtag tttgcgcatg ggaggctcct gtaatgcgtt acagactact
     3781 ttagagcatc gacgccaaat aaaaaggcca accgtcaggg aaggctggcc atggcagaat
     3841 ttagaacgag gcccccaaac gcaggtagac ggtggtttcg ccgatatcct cgtcaagccc
     3901 cttggcaaaa ccgatcgtgc tgtcgagctt gaaggtgctg taaccgacca gcaggtcagg
     3961 ctttatctca aaaccggcac cggtgtaata gtggtggcct tcgctcttgt cccaggccgc
     4021 gccatgatcc acgaacaggt gcatggcggc cttgccgatg cccagcggaa acgtggtgaa
     4081 accgtcgaaa tgctccagca gcgggaagcg ccattccgcc gaatacagtc gcagattggt
     4141 gccgctgagc tcgtcatagc catcattgta accacgcaag gtatagccgg ttttgccgaa
     4201 gccgatgttg ccacccatgg attcaaactg gtccagctcg ttgcccagct cgtagggttt
     4261 ggtgtcgtca ccggcgcggc cggccactgc gcggatcgcc agcacatggt tgttgatcag
     4321 gctgatgtat tcgcgccagt ccagcgtctt ggcttcgcct tcgtgatagg cgccgcccag
     4381 cagattgtat ttttcgtaat tgagtttgat ccagcgacca tcctcgacac tgatggaatg
     4441 cagataattg tcgtagtcgg cccagctcag cgcgatgccg ccgaagttgt cgttgaagtc
     4501 ggcgatctcg ccgtaatttt ccatttcccg caccacgttt tcggtgccca cgccggtatc
     4561 gatgcggaag gtgccgtcga aactgttgaa cggttgcatc cacacggcct ggtaacgggt
     4621 ttcggtgtcc cactgctcca gcacgccggg ctcggcttcc acttcaacat ccaccgtgga
     4681 atcccacagc agcgccaggc gatgaaatgc gatgtacgcc gcactgccac ccactttgtc
     4741 cttgtcgaga taaaacgtcg gcgccagctg ccagaagtgg taacccagca cgtcctgtcc
     4801 gttcaccaga aactgcagcg cggtgttgtc ctgggtgtcg gcatacagca gtgcaaacca
     4861 gccatgcggc gcaatggtac tcagcgcgga ataatccgtg ctgtcgctgt actgatccgg
     4921 attgaaaccg gtctgattga caatggtggg caatgccgcc ggttgcgcgg tcaccgctgc
     4981 atacacggtc tgctgcggca atattttctg ctggcggagc attacaccat cggccgtgta
     5041 ttccgcgaca cgcatctgtg cagtggtttc gtccagcgca taatccacta tcgccgtttg
     5101 cgtgtgggaa acggtctcga cccggccgct ggccatatcc agccgccgca cattccatag
     5161 gtgattctgg tcggaaataa aatagaccga tttgccgtcg gcggaaaagc gtggctgctg
     5221 ttccagatcg gaattggcgg tgagaggccg ccagttgccg gtggcaagat ccagcagctc
     5281 cagattccag ccggaggccc tgcgccggat tgcagccacc agctggcgat cgtcaggcga
     5341 ccaggcaatc tgcccgatgg tgtcaccggc aggcagggct ttcagcaatt gttgcagctc
     5401 gccttcgcca tccagcagcg cgatctggtt ctgtccgccc tgcacatgca cggcggcgat
     5461 ctgctcaccg ctggtggacc aggcaatgct cggatagcga ccgcaatgcg taatgcggcg
     5521 ccaacgtccg ttgtccttca gccggtacag atcggtgtac accttgcggt tgtcgcacac
     5581 atcctcgcgg gccagcagca caccggcgcg gggatgcaca tcgaactgca cgaagcgatc
     5641 cactttcgca acccgggttt ccacgccctg cgcatcgatg cgttgcagac tgggtttgtt
     5701 gcggccgtcg ttgcggtagt aatagaaatc gccgccgggc agccactccg gattggtgtt
     5761 gatgcggcca tcgctcacca ccgccgtctg ttcaccggcg ggaatctgtt tcacctgcgc
     5821 ggagaatttt tcattcagat aatcgatgta ctgctgccag agcgcttcgg cgctgatgtt
     5881 gaaggtgtcc tgcgcgcggc tgttcatgcg ccacggaatc aggttgctgt tccagttttt
     5941 cagataggcc attgcctttt catggccatg acgctgctcc aggaactcaa agaaatagta
     6001 accgtacaga taaacctggc ccgacggcca gtcggtgccc cagtagccct ggtagctggt
     6061 ctgggaatag gtgcgaaaac ctttgctgta ttccgcccgc atcatcgcgt cgtacagcgc
     6121 gctctggccg cgaccgaaac ctttctgcgc atcggtctcg cccttcacag cgattccctc
     6181 ggtaatccac agcgggctga aggcttgcgg gaagttgaag aatatccggc caaacacatt
     6241 gcgcaggccg ctgggcaaac cgtcggcctg atccagatgc aggatgtgca catattcgtg
     6301 agtgaacacc tgctcgatcc agtcgccgtt gtcgagcagt tcgccgtcgg tgggcgcgtt
     6361 catgaaaatg aaaaaacggt tatagggcat gacggatgcg ccgccatt
//
LOCUS       DASUEG010000078         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1875920, whole genome shotgun sequence.
ACCESSION   DASUEG010000078 DASUEG010000000
VERSION     DASUEG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875920"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1139)
                     /locus_tag="VFX11_01270"
     CDS             complement(129..1139)
                     /locus_tag="VFX11_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4937277.1"
                     /translation="MKNGLKLGIALGSGSARGWSHIGVLRALKRYGIEPDIVAGTSIG
                     ALVGACYAGGTLDELERWVTSLGWRDVVSLLDLKLGGGLIEGRKVFQFFEQHMSELTF
                     EQLQKPFAAVATDLETGREIWLQHGHLAEAVRASLSIPGLFRPYRSQAGRILVDGGLV
                     NPVPVALCRAMGADLVIAVNLNSEIVGKHLRNSRKASTEFQPQNGSADGDESDKPAAP
                     ADAVRSGESDFMRRLTQLFADEGSDNVSSSAGSNGGSNDRNQPMPGLMEVIASSINIM
                     QDRVTRSRMAGDPPDLVLTPRLSHLGLMEFYRAAEAIAAGEQTVEHALPALRTLLGLD
                     ET"
     gene            complement(1136..2191)
                     /locus_tag="VFX11_01275"
     CDS             complement(1136..2191)
                     /locus_tag="VFX11_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4937278.1"
                     /translation="MTKTRGILLLTVVCAGLLACARDLPADWHSLRRLLQAETLSQPV
                     SPPLTVQFLGAGGIYLHYRDQSLLGDPFFSNPPISQWITLRNLKSRPDVIDAHLPPLD
                     QVQGILVGHGHFDHAMDVPYIAQKLPASVKVYGSDSTRNLLAPELPLERLVGLDGKMA
                     QQQAGGEWIYLTSTLRILPIYSEHAPHFGETIVASGNIQHPLQERAGDALDWKAGVNL
                     NYVIDFLEPGTGGAVTSVAYRLFYQSSSSSAPIGFPPQWLLEDGVPFDLALVCAANYS
                     HVKDYPQGLLAYIKPREVLLIHWEIFWDDYSTTTASPMPGMDFADLEQRIRSVLGKDV
                     PVRVPNRGSSFELGGRS"
     gene            complement(2319..>2453)
                     /gene="purT"
                     /locus_tag="VFX11_01280"
     CDS             complement(2319..>2453)
                     /gene="purT"
                     /locus_tag="VFX11_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403797.1"
                     /note="non-folate utilizing enzyme, catalyzes the
                     production of beta-formyl glycinamide ribonucleotide from
                     formate, ATP, and beta-GAR and a side reaction producing
                     acetyl phosphate and ADP from acetate and ATP; involved in
                     de novo purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase 2"
                     /protein_id="HEX4937279.1"
                     /translation="QLRLFGKPEVKGSRRMGVGLALADTVDAAVQKALRVANAVDVKL
                     "
BASE COUNT          472 a          788 c          724 g          469 t
ORIGIN      
        1 tgatccttga actgtaactt cagcatgtcc ctcgatgact cccagacgtt gaaggcagga
       61 ttgaatgact gccgcttagt atagtgggca cgggccccgc ctgttaagaa acctctccaa
      121 caatcggctc aggtttcgtc cagccccagc agggtacgca gggccggcaa cgcgtgctca
      181 acagtctgct cgccggcggc gatggcttca gctgctcggt agaattccat cagacccaga
      241 tgcgacagcc ggggagtcaa caccagatcc ggcgggtcac cggccatgcg gctgcgggtg
      301 acgcggtcct gcatgatgtt gatggacgat gcgatcactt ccatcagtcc cggcatgggc
      361 tggttacggt cgttgctgcc accattactg cctgcgctgc tgctcacatt gtcgctgcct
      421 tcatcagcaa acagctgcgt gaggcggcgc atgaaatcgg actcaccgct gcggacagca
      481 tcggcagggg ccgctggctt gtcggattca tcaccgtccg cactgccatt ctgcggctgg
      541 aactcagtac tggccttgcg ggaattgcgc aggtgcttgc cgacaatttc gctgttgagg
      601 ttgaccgcga tcaccagatc ggcacccatg gcgcgacaca gggcgaccgg caccggattc
      661 accagccctc cgtccaccag gatgcgtccg gcctgggaac gatagggccg gaacaggccg
      721 ggaatcgaca gggacgcccg cacggcctct gccaggtggc catgctgcag ccagatttcg
      781 cgaccggttt ccagatcggt ggcgacggca gcgaagggtt tctgcagttg ctcgaaggta
      841 agctccgaca tgtgctgctc gaagaactgg aacaccttgc ggccttcgat caggccaccg
      901 ccgagcttca ggtccagcag gctcaccaca tcccgccagc ccaggctggt gacccagcgt
      961 tccagctcat ccagcgtgcc gccggcatag caggcaccga ccagcgcgcc gatactggtg
     1021 cccgcgacga tgtccggctc aataccgtaa cgcttcagcg cccgcaacac accaatgtgg
     1081 gaccagcccc gcgccgaacc actgcccagt gcgattccca gtttcaaccc gtttttcatg
     1141 accgtcctcc cagttcgaac gaactgcccc ggttcggcac ccgcaccggc acgtccttgc
     1201 ccagcaccga acggatacgc tgttccagat cggcaaaatc cattcccggc atcgggctgg
     1261 cggttgtggt gctgtagtcg tcccagaata tttcccagtg gatcaacagg acttcgcgcg
     1321 gtttgatata tgccagcagg ccctggggat aatccttcac gtggctgtaa ttggcggcgc
     1381 agaccagagc cagatcgaac ggtacgccgt cttccagtaa ccactggggc ggaaaaccga
     1441 tgggcgcact gctggaggag gactggtaaa aaagccggta ggccacgctt gttactgcgc
     1501 caccggtgcc aggttcaaga aaatcgatga cgtagttgag attgaccccg gccttccagt
     1561 ccagcgcatc ccctgcccgc tcttgcaatg ggtgctggat gttgccggac gccacgatgg
     1621 tctcgccaaa atgcggcgcg tgctcggaat aaatcggcag gatgcgcagg gtggacgtca
     1681 ggtaaatcca ttcaccgcca gcctgctgct gcgccatttt cccgtccagc cctaccaacc
     1741 gttccagcgg cagttccggc gccagcaggt tgcgggtgct gtcgctgcca tagactttca
     1801 cgctggccgg cagcttctgc gcgatgtagg gcacatccat ggcgtgatcg aaatggccgt
     1861 gtcccaccag gatgccctgc acctgatcca gcggcggcag gtgagcgtcg atgacgtccg
     1921 gccgcgactt gagattgcgc aacgttatcc actgggaaat cggcggattg gagaaaaagg
     1981 gatcgcccag caggctttgg tcacggtagt gcaggtagat gccgccggca ccgagaaact
     2041 gaacggtgag cggcggcgag accggctgcg atagcgtttc agcctgcagc aaccgccgca
     2101 ggctgtgcca gtcggcaggc aggtcgcggg cgcaggccaa caggccggca cacaccactg
     2161 tcagcagcag gattccccgt gtttttgtca tgtgtgctcc tggtgccgtt cgattcaaat
     2221 cgtgctccct ttcaggaagt ttcctttcaa gaaattcccg tcaggacatt ctctgcgaga
     2281 aaatgcctgc ctcacgctcc cggctactcg gcgccgcctc agagtttcac atccaccgcg
     2341 ttcgccaccc gcagcgcttt ctgcacggcg gcgtcgacgg tatccgccag cgccagacct
     2401 acgcccatgc gccggctgcc tttcacttcc ggtttgccga acaggcgcaa ctg
//
LOCUS       DASUEG010000079         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1652961, whole genome shotgun sequence.
ACCESSION   DASUEG010000079 DASUEG010000000
VERSION     DASUEG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652961"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFX11_01285"
     CDS             <1..301
                     /locus_tag="VFX11_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4937280.1"
                     /translation="GQVRFGPDTAYVAQEDYRVTDDLRADFARAIRHYFPALDESRLI
                     PAYAGIRPKIQGPGEPAQDFRIDQPLPGLINLFGIESPGLTAALAIARHVADRMA"
     gene            331..1482
                     /locus_tag="VFX11_01290"
     CDS             331..1482
                     /locus_tag="VFX11_01290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4937281.1"
                     /translation="MSDRAPSCQVTYQVDYQVTIGAPDRDALPRLWQSLEARATPNFF
                     LSWLWVDAWLTTFNPECRLVQIQCDSVLVGLGLLTCNEEIRHRILRSRMLRLGRTGNA
                     VQDQIWIEYNGLLLDRQHEHSAPAALLQQLLQQDDWDEFELGASDAAVLQRYAHPDCT
                     AIIRWSAPAYGVNLAALREAKTDYLASLSRNTRYQINRSRKLYAADATLTFSTHTAPD
                     AILNLWPQLADLHRARWGNAPGQSGFANDAFVRFHQHLIRHGGADDRVELCTLHRNDE
                     LLGCLYNFLYKGRVYFYLSGLRAETDPHLKPGLLLHAMAIQHYLERHYDYYDFMGGEA
                     RYKQSLGNQHCELQLVSFQRPRLKLKLEQLARRIKHLTPDKQSTDDHQQ"
     gene            1466..2992
                     /gene="xrtA"
                     /locus_tag="VFX11_01295"
     CDS             1466..2992
                     /gene="xrtA"
                     /locus_tag="VFX11_01295"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEX4937282.1"
                     /translation="MTISNSETQDQALEGFPLRTFVFAVTVWLLAWLLVFHDALLSAA
                     SVWLINDTFNHCFFVLPVVLYAIWQQRGAVLAQAPRYSALGGLLVLGALLTLALGQAA
                     FIDVLQHLAVFGLLPAMVLFLLGWKVVRVIWFPLLFVLFSVPLGEELIPQFQQITADI
                     AIALLHLIGVPVYRDGLYISVPNGHFVVAEACSGVRFFIACVVLGTAYAYLNFVSRWR
                     ALAFALFSVVMPIIANGIRAFGIIYIGHATNMKHAAGADHLVYGWFFFAVVVVLLLVT
                     GHFFSDGHRPWQNRITTIDPGWRRHASRTTLLLAAAPLLLALTLKLVQDQYTETAFDL
                     DSAGLNSVSKEQAQGQDWTPRLDTADEFRLGRDTATGAQLFQALYRHNEAGRELVSWA
                     NRVYDIDQWSLKEQTTQTVDGLGAVTVLDLTSLRNRQRLVAYWYVVPNRISSSQTRIK
                     LQQAINTLLLQPSGGGLVAVSLDYRGNTETAHAQLQQVLDNNARHLSGLLQPGQPTSE
                     "
     gene            3026..3850
                     /locus_tag="VFX11_01300"
     CDS             3026..3850
                     /locus_tag="VFX11_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEX4937283.1"
                     /translation="MQHAMTIDVEDYFQVSAFENLVKPKDWDSMTPRVEANTDKLLQL
                     FADHNTTATFFTLGWIAQRFPAMIRRIVAQGHELASHGTLHQRASAQTREEFKADVGD
                     AKRLLEDLSGKPIVGYRAPSFSFTKNNLWAYEVLAEEGYRYSSSVYPVVHDHYGIPDA
                     PRFRYHTDSGIDEIPLSTLPLFGRNLPISGGGYFRLYPYALTRWAVSRFSSKETQPYI
                     FYMHPWEVDPNQPRMQGASAKSRFRHYLNLKRVEARLTHLLDDFEWSSMAKLYGYS"
     gene            complement(3971..>4081)
                     /locus_tag="VFX11_01305"
     CDS             complement(3971..>4081)
                     /locus_tag="VFX11_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4937284.1"
                     /translation="VGKKCIIGAGSVVTDDVEDYSIVVGNPAKVVKKRNN"
BASE COUNT          785 a         1304 c         1199 g          793 t
ORIGIN      
        1 gggccaggtg cgttttggac cggacacagc gtacgtcgcc caggaagact atcgcgtgac
       61 ggacgatctg cgcgccgact ttgcccgggc cattcgccat tactttcctg cgctggatga
      121 atcccgcctg attccggcct atgctggaat caggccgaaa atccagggcc cgggtgagcc
      181 ggcgcaggat ttcaggatcg accagccgct gccggggctg atcaatctgt ttggcatcga
      241 atcgccggga ctgacggctg ccctcgccat cgcgcggcac gtcgccgacc ggatggcatg
      301 aatgaaccgc cgcaacccga aaggaccaac gtgagcgacc gcgccccttc ctgccaggtg
      361 acttaccagg tggattacca ggtgactatc ggcgcgcccg accgcgacgc actgccgcgc
      421 ctgtggcaat cgctggaagc acgggcgacg ccgaatttct ttctgtcctg gctgtgggtg
      481 gacgcctggc tgaccacctt caatcccgag tgccgcctgg tgcagatcca gtgcgattcc
      541 gtgctggtcg ggctcggatt gctcacctgc aatgaggaaa tacgccaccg cattctgcgc
      601 tcgcgcatgc tgcgtctggg aagaacgggt aacgcagtcc aggatcagat ctggatcgaa
      661 tacaacggcc tgctgctgga ccggcaacat gaacacagcg caccggcagc actgctacaa
      721 cagttgctgc agcaagacga ctgggacgaa ttcgaactgg gcgccagcga cgcagcggtg
      781 ttgcagcgct atgcccatcc ggattgcacc gccatcatcc ggtggtcggc accggcctat
      841 ggtgtgaatc tggccgcatt gcgggaggca aaaacggatt acctcgccag cctgtcccgc
      901 aacacgcgct accagatcaa tcgcagccgc aagctgtacg cagccgatgc cacactgaca
      961 ttcagcacgc acaccgcacc ggatgccatc ctgaacctgt ggccgcagct ggcagacctg
     1021 caccgggcgc gctggggcaa cgcacccggg cagagcggct ttgccaacga cgcgttcgtg
     1081 cgcttccatc aacacctgat caggcatggc ggcgcggacg acagggtgga actctgcacc
     1141 ctgcaccgca acgacgaact gctgggctgt ctgtacaatt ttctgtataa gggccgcgtg
     1201 tatttttatc tgagcgggct gcgcgccgaa accgatccac atctgaagcc gggcctgttg
     1261 ctgcatgcca tggcgatcca gcactatctg gagcggcatt atgactatta cgatttcatg
     1321 ggcggcgagg cacgctacaa gcagtcgctg ggcaaccagc attgcgaact gcagctggtc
     1381 agcttccagc gcccccggct caagctgaaa ctggaacagc tggcccgccg catcaaacat
     1441 ctgacaccgg acaagcagtc aaccgatgac catcagcaat agcgaaaccc aagatcaggc
     1501 cctggaaggc ttcccgctgc gcacattcgt atttgcagtg acggtctggc tgctggcctg
     1561 gctgctcgtt ttccatgacg ccctgctgtc ggccgccagc gtctggctga tcaacgacac
     1621 cttcaatcac tgctttttcg tgctgccggt ggtgctgtac gccatctggc aacagcgcgg
     1681 tgccgtgctg gcgcaagcgc cgcgctattc cgcactgggt ggactgctgg tgctgggtgc
     1741 gctgctgacc ctggccctgg ggcaggccgc gttcatcgat gtgctgcaac acctggccgt
     1801 attcggcctg ctgccggcaa tggtgctgtt tctgctgggc tggaaggtgg tgcgcgtcat
     1861 ctggtttccc ctgctgttcg tgctgttcag tgtgccgctg ggggaagaac tgattcccca
     1921 gttccagcag atcaccgccg acatcgccat cgccctgctg cacctgatcg gtgtgcccgt
     1981 gtatcgggac ggactctata tcagcgtgcc caatggccat ttcgtggtgg ccgaagcctg
     2041 ctccggcgtg cgttttttca ttgcctgtgt ggtgctgggt acggcctatg cctatctcaa
     2101 cttcgtctca cgctggcgcg cactggcgtt tgccctgttt tcggtggtga tgccgattat
     2161 cgccaacggc atccgcgcct tcggcatcat ctacattggc cacgccacca acatgaagca
     2221 cgcggccggg gccgaccacc tggtgtatgg ctggtttttc ttcgcggtgg tggtggtgtt
     2281 gctgctggtg acagggcact tcttttccga cggccatcgc ccgtggcaaa accggatcac
     2341 gacgatcgac cctggctggc gccgccatgc ctcccgcacc accctgctgc tggccgccgc
     2401 ccccctgctg ctggcgctga ccctgaaact ggtgcaggac cagtacacgg aaaccgcctt
     2461 cgacctggac agtgccggac tgaacagcgt cagcaaggaa caggcacagg gtcaggactg
     2521 gacgccccgc ctggacaccg ccgacgaatt ccgcctgggg cgggataccg ccaccggcgc
     2581 ccagctgttc caggccctct accggcacaa cgaagcaggg cgggaattgg tgagttgggc
     2641 caaccgcgtt tatgatattg atcagtggtc actcaaggag cagaccaccc agacagtgga
     2701 cggtctcggt gccgtcaccg tgctggacct cacctcgctg cgcaaccgtc agcggctggt
     2761 ggcttactgg tatgtagtac ctaacaggat tagcagcagc cagacccgga tcaagctgca
     2821 gcaggccatc aacacgttgc tgctgcagcc ctccggtggc gggctggtcg ccgtcagcct
     2881 ggattatcgc ggcaacactg aaacggctca cgcgcaactg caacaggtgc tggacaacaa
     2941 tgcacggcac ctgtccggcc tgctccaacc cggtcaacca acatctgagt gattcagggg
     3001 ccaacgccct tacggagacc ccaccatgca acacgccatg accatcgacg tggaagacta
     3061 cttccaggta tcggcctttg aaaacctggt caagcccaag gactgggaca gcatgacacc
     3121 ccgcgtcgag gccaacaccg acaagctgct gcaactgttc gcggaccaca acaccaccgc
     3181 cacctttttc accctgggct ggattgccca gcgcttcccc gccatgatcc ggcgcattgt
     3241 cgcccagggc cacgaactgg ccagccacgg caccctgcac cagcgcgcca gcgcgcagac
     3301 gcgggaggaa ttcaaggcgg acgtgggaga tgccaagcgg ctgctggagg atctgtcggg
     3361 aaagcctatc gtcggttatc gcgcgccgag tttttccttc accaaaaaca atctgtgggc
     3421 ctatgaggtg ctggcggagg aaggttaccg ctacagttcc agcgtgtacc cggtggtgca
     3481 tgatcattac ggcattccgg atgcgccgcg ctttcgctac cacacggatt ccggcatcga
     3541 cgaaattccg ctgagcacgc tgccgctgtt cggccgcaac ctgccgattt ccggcggcgg
     3601 ttacttccgg ctgtacccgt atgcgctgac gcgctgggcg gtgagccgtt tcagcagcaa
     3661 ggaaacccag ccctatattt tttacatgca cccgtgggaa gtggacccga accagccgcg
     3721 catgcagggc gcgtcggcga aaagccggtt ccggcattat ctgaacctga agcgggtgga
     3781 agcacgcctg acccacctgc tggatgattt cgagtggtcg tcgatggcga agttgtatgg
     3841 ttacagttga aggccgaaac aaacaaaacc cacttgaact tcaaatctgg acacgccgtg
     3901 aacccatccc tgggggctgc tccgcggcat ccatgccgca gagcgtccag atttgaaatt
     3961 caagtgggct tcagttgttt ctttttttca cgaccttcgc cggattcccc actacgattg
     4021 aatagtcctc cacatcatcg gtgacgaccg aaccggcgcc gatgatgcac ttcttgccaa
     4081 c
//
LOCUS       DASUEG010000080         6716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_173139, whole genome shotgun sequence.
ACCESSION   DASUEG010000080 DASUEG010000000
VERSION     DASUEG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173139"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VFX11_01310"
     CDS             <1..1460
                     /locus_tag="VFX11_01310"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4937285.1"
                     /translation="TAPTTLDAWVRRLGEEEFPLFAHTARSIAAMSTDDGSSTSELAQ
                     VILRDNTMTARVLKMANSVYYNPGGVRINTVSRAIVILGFDTVRNIALSIGLVETMLS
                     GSRHDNALAELVRSFHAAVQAKELAEQSKLGNTEELFIAALLYRIGAVAFWCFPYGKA
                     DALEWQYHNTADREEAERRVLGFTLRNLTLALIRDWHLSALLATALASGNNRERNLRE
                     VELAYRLADAAEQGWNHPDVTTVLHNIGSMLGRNNEWVTRQVYLNAQQAAKVMSDFGI
                     VNTDRFIPPPPGESPELVPAIAAAPDPQLMLQLGILRELSALLSEKADLNVVLSTVLE
                     GIYRGLKMDRVVLAMLDLRQPRLVARFVLGKERETLLQKFQVTMPSPPPATGDGREQI
                     FSHVLAQNEPVWIDRRTRQQLGGLFVPELQALLGPGEFFVAPVRVQGQPRGVIFADRS
                     PANGPLTEQEFLTFRHFCDHANIAFNLLSSEVRRS"
     gene            complement(1464..1763)
                     /locus_tag="VFX11_01315"
     CDS             complement(1464..1763)
                     /locus_tag="VFX11_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937286.1"
                     /translation="MKNLEQIAELALRQLDTAGYYLGRYGISDRINRHNRIALVMTRK
                     ERVRGELSRLELRAALQRKRLQQTLDQVSRSADQLIDLAPAPLAHQLHRAKARVI"
     gene            1997..2500
                     /locus_tag="VFX11_01320"
     CDS             1997..2500
                     /locus_tag="VFX11_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX4937287.1"
                     /translation="MTRALCCLLIGLAPAQIQAEDIYQIKAAFIYNFTKFVTWPEEME
                     LRGGDLRLCIFDRNPFGEYLYQLSGRKVRNFELKVVQPASVSELEGCAILFVAGTVKS
                     ATVLRGLTNHPVLTVSDQSGFVQDGGVIELLSENNRIRFDINLQAVRDSGLQMSSKLL
                     QLARQVR"
     gene            2500..3930
                     /locus_tag="VFX11_01325"
     CDS             2500..3930
                     /locus_tag="VFX11_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4937288.1"
                     /translation="MHGFWRRFSFKQKLTLMMLFTIASGVVLIGLFFVVYERSHMRDQ
                     LEHEIDVVSRVAIAQLSAALMFDDARTLQESVNSLRFDNTVEVACIYAASGELKVHSF
                     PGDNNPVDCPSSPDTIVTGFMDDHYEMRKTIYMDDTLLGSLYVRANLDYVDQHMRRYI
                     YMMLTALAVISVVSLLVAMWLQQVITHSITRLAETAGRIAHQNDYSLRAEKTSEDEIG
                     QTVDAFNRMLSVIEQSDKELKQNKVELDTIVSERTAELRTANRELEAFSYSVSHDLRS
                     PLRAIDGFSQALLEEYSDVLDDVGKSYLDRVRAATQRMGLLIDSLLRLSRVTRQEIVP
                     EPTDLSAMARDIVRELQEEYPSRMVSVEIQDGMVASCDAKLMRIALTNLIDNAWKYSA
                     KQAVASVAMGCVDKVYFVRDNGAGFDMRYADKLFGAFQRLHTKEEFEGTGIGLATVAR
                     VIHRHGGDIWAESEPGKGATFHFTLG"
     gene            4082..4513
                     /locus_tag="VFX11_01330"
     CDS             4082..4513
                     /locus_tag="VFX11_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937289.1"
                     /translation="MTILLVEDNADDELLALRALKKTSVPNHVVVARDGEEALEYLFG
                     RGKYEGRNVGEQPKVVFLDLKLPKLNGIDVLRSIRMDNRTSRMPVVLLTSSDEPQDLI
                     DGYDSGANSYINKPVDFNEFVEQVKLLGQYWLGVNRVPALY"
     gene            4537..6219
                     /locus_tag="VFX11_01335"
     CDS             4537..6219
                     /locus_tag="VFX11_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4937290.1"
                     /translation="MDRLLKVLIADDSEDDALLLVRQLKKGGLTCWFERVDTAEDMRV
                     SLNRQHWDLVVTDHNMPAFSSAEALQLVKAIQPDTPVIVVSGAITDDVAVNSMKAGAQ
                     DYIMKDNLARLVPAVERELRDAEHRNARKEAEQQVAYLIYHDSLTDLMNRDQFKKQLE
                     TVLENARVHGHEHALLYVDLDQFKIINDTCGHVAGDELLKQIGKVIKGELREQDRVSR
                     MGGDEFGILLWQCNLDEALEAARKVHNAIKEFRFLWNEQRFVISSSIGVVQLNSASTD
                     VEEVLASADMACYAAKDKGRSYIQVFDAHDRELTQRRDQMHWASRIRRALDENAFVLY
                     SQAIVGLAQPARHEEFLVRMRDGDTIIAPGMFMPAAERFNLMPLIDRWVIRNAFAHVK
                     KRLEQNRLADGDLFFINLSGSSLSDESFFEFVHQCREEFELPQRKICFEITETTAISN
                     LETASQFISEIRDLGFLFALDDFGVGMSSFAYLKAIPVDVLKIDGGFVRNILNDPMDQ
                     AIVEACNNIAHSAGLITVAEFVENKAIEDRLRELGVDFGQGYGIEKPRPIDA"
     gene            6212..>6716
                     /locus_tag="VFX11_01340"
     CDS             6212..>6716
                     /locus_tag="VFX11_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937291.1"
                     /translation="MPETTTARTLVFALASLLAVSAQADTYSSPPHSGSSLAVAPQPV
                     SDAAPLVAYAPDFLPQEWPWIDTSVELEDLPEEEELPEAEPEGYMDRSVDYLLEQRKS
                     ISTRVNVMARAIDRYLAGDRAMAEENETFMKLQLAERWIEGGKWDPDNDLKFRLDLPA
                     TKRRYRLV"
BASE COUNT         1370 a         1878 c         2111 g         1357 t
ORIGIN      
        1 tgaccgcgcc gaccaccctg gacgcctggg tccgtcgcct gggggaggag gagttccccc
       61 tgtttgccca tacggcgcgc agtattgcgg ccatgtcgac cgacgatggc tcgtccacca
      121 gcgaactggc ccaggtgatc ctgcgcgaca acaccatgac cgcgcgggtg ctgaaaatgg
      181 ccaacagcgt ctactacaac cccggtggcg tgcgcatcaa tactgtgagc cgtgccatcg
      241 tcattctggg ttttgatacg gtgcgcaaca ttgcgctgtc gatcggactg gtggagacca
      301 tgctgtccgg ctcccgccac gacaacgcac tggcggaact ggtgcgatcc tttcatgcgg
      361 cggtgcaggc caaggagctg gcggagcagt cgaagctggg caataccgag gaactgttca
      421 ttgccgccct gttgtaccgc atcggtgcgg tggcattctg gtgctttccc tatggcaagg
      481 ccgatgcgct ggagtggcag taccacaata ctgccgaccg cgaggaagcc gagcgccggg
      541 tgctgggatt caccttgcgc aacctgacgc tggcgctgat ccgcgactgg cacctgagcg
      601 ccctgctggc cacggcgctg gcgtcgggca acaatcgtga gcgcaacctg cgtgaggtgg
      661 aactggctta ccggctggcg gacgcagcag aacagggctg gaaccacccg gatgtcacca
      721 cggtgctgca caacatcggc agcatgctgg ggcgcaacaa cgaatgggtg acgcggcagg
      781 tgtatctgaa tgcgcagcag gcggcgaagg tgatgtcgga tttcggcatc gtcaatactg
      841 accgttttat tccacccccg ccgggcgagt cgccggagct ggttcccgcc atcgccgccg
      901 cgcccgatcc ccagttgatg ctgcaactgg gaattctgcg ggaactgtca gcgctgctga
      961 gtgaaaaggc ggatctgaat gtggtgctga gcacggtgct ggagggcatt taccggggtc
     1021 tgaagatgga ccgggtggtg ctggcgatgc tggatctgcg gcagccgcga ctggtggcgc
     1081 gattcgtgct gggaaaggag cgggaaaccc tgctgcaaaa gtttcaggtg acaatgcctt
     1141 cgccgccacc agcaacgggt gatggccgcg agcagatttt ttcccacgtg ctggcgcaga
     1201 acgaaccggt gtggatcgac cgccgcaccc gccagcagct gggtggactg tttgtgccgg
     1261 agctgcaggc cctgcttggc ccgggcgagt ttttcgtggc gccggtgcgg gtgcagggcc
     1321 agccgcgcgg tgtcatcttt gccgaccgca gccctgccaa cgggccgctg accgagcagg
     1381 aattcctgac attccggcat ttctgcgatc atgccaatat cgcgttcaac ctgctcagca
     1441 gcgaggtgcg tcggtcctga gggtcagata acccgcgcct tggcccggtg cagctggtgt
     1501 gccagcgggg cgggggccag atcgatcagc tggtcggcac tacggctgac ctgatccagt
     1561 gtctgctgca ggcgcttgcg ctgcagtgcc gccctgagct ccaatcggga cagctcgccc
     1621 cgtacccgtt ccttccgggt cattaccagg gcaatccgat tgtgacggtt gattcggtcg
     1681 ctgatgccgt agcgacccag gtagtagccg gcggtgtcca gctgacgcag cgccagttcg
     1741 gcaatctgtt ccagattttt catggggtac ctgcaagtag ggggtgggtt aaccgggtgg
     1801 ataaacatga atgcggcgca atgtagacgg atttattagg ggaatccacc taaccctgag
     1861 cgctggaatc ccggcgccgc cccgggcgac aggcacgaaa aaagtgcact atagtgaagt
     1921 gcgtacacca aggaattcac caggatagat gactaaggga taccggcaag ggacaggtag
     1981 gcggggcggc agctggatga cgcgggcttt gtgttgcctg ctgattggcc ttgctcctgc
     2041 acagattcag gccgaggaca tctaccagat caaggcggcc ttcatctaca acttcaccaa
     2101 gttcgtcacc tggcccgagg agatggaact gcggggcggt gacctgcgtc tgtgcatttt
     2161 cgatcgcaat ccctttggtg aatatctcta tcagttgtct ggccggaaag tccgcaattt
     2221 cgaactcaag gtggtccagc ccgccagcgt cagcgaactg gaaggctgcg ccattctgtt
     2281 tgtggccggt acggtgaagt ccgccacggt gctgcgaggg ctgaccaacc atcctgttct
     2341 caccgtgagc gaccagtccg gcttcgtcca ggacggcggt gtcatcgagc tgctgtccga
     2401 aaacaaccgc atccgtttcg atatcaacct gcaggctgtt cgcgacagcg ggctgcagat
     2461 gagttcgaaa ctgctgcaac tggccaggca ggtgcgctga tgcacggatt ctggcgccgc
     2521 ttctccttca agcagaaact caccctgatg atgctgttca ccatcgccag cggcgtggtg
     2581 ctgatcggat tgttcttcgt ggtgtatgaa cgttcccaca tgcgcgacca gctggagcac
     2641 gagatcgacg tagtgtcgcg ggtggccatc gcgcaactgt cggcggcgct gatgtttgat
     2701 gacgcccgca ccctgcagga aagcgtcaac agcctgcgct tcgacaatac ggtggaagtg
     2761 gcctgcatct acgcggccag tggcgaactg aaggtgcatt cgttccccgg cgacaacaat
     2821 ccggtggatt gcccgtcatc gccagacact atcgtgaccg gtttcatgga tgatcattac
     2881 gaaatgcgca aaaccatcta catggacgat acgctgctgg gcagccttta cgtgcgcgcc
     2941 aatctggatt atgtcgatca gcacatgcgg cgctacatct acatgatgct gacggcgctg
     3001 gcggtgattt ccgtggtgtc gttgctggtg gcgatgtggc tgcagcaggt catcacgcat
     3061 tcgatcaccc ggctggcgga aacggcgggt cgcattgcgc accagaatga ctactcgctg
     3121 cgggcggaaa agaccagtga ggacgaaatc ggccagaccg tggatgcatt caaccggatg
     3181 ctgtctgtga tcgaacagag tgacaaggaa ctcaagcaga acaaggtcga actggacacc
     3241 attgtcagcg aacggacggc ggagctgcgc actgccaacc gcgagctgga agcgttcagc
     3301 tactccgtgt cccacgattt gcgttcgccc ctgcgcgcta tcgacggctt cagccaggcc
     3361 ttgctggagg aatacagcga tgtgctggac gatgtcggca agagctatct ggatcgcgtg
     3421 cgtgccgcca cccagcgcat gggattgctg atcgacagcc tgctgcggct gtcgcgggtg
     3481 acacggcagg aaatcgtgcc ggagcccact gacctgtccg ccatggcacg ggacattgtg
     3541 cgggagctgc aggaggagta tcccagccgt atggtgagtg tcgagatcca ggatggcatg
     3601 gtggccagct gcgacgccaa gctgatgcgg attgcgctga cgaatctgat cgacaacgcc
     3661 tggaagtaca gtgccaaaca ggcggtggcg tcggtggcga tggggtgcgt ggacaaggtt
     3721 tattttgtcc gcgacaacgg agccggtttc gatatgcggt atgcggacaa gcttttcggc
     3781 gcgttccagc gcctgcatac caaggaggaa ttcgaaggca ctggcattgg cctggccacg
     3841 gtagcgcggg tgatccatcg ccatggcggg gatatctggg ccgaaagcga acctggcaaa
     3901 ggcgccacct tccatttcac gcttggctga gcgctgttcc ggtcagcttc aaatgacaaa
     3961 ttcctgacat gggtgaatga aaagcgtcgg agatgttgac ctttccggcg actggacgta
     4021 acgtcgttaa tgctcgcagt catcttcgat atttcgtcct ggaataataa gaatggatcc
     4081 tatgaccata ctgctagttg aagataacgc cgatgatgaa ctgttggcgc tgcgagcatt
     4141 gaaaaaaacc agtgtcccca atcatgttgt cgtggcccgc gacggggaag aagcgctgga
     4201 atacctcttc gggcgcggta aatacgaggg acgcaatgtg ggtgaacagc ccaaggttgt
     4261 gtttctggat ctgaaacttc ccaagctcaa tggcattgat gtgttgcgtt ccatccgtat
     4321 ggacaaccgt accagccgga tgccggtggt actgctgaca tcctcggatg aaccgcagga
     4381 tctgatcgac ggctacgaca gcggtgccaa cagctatatc aacaagcccg tggatttcaa
     4441 tgaatttgtc gagcaggtca agctgctggg ccagtactgg ttgggtgtca atcgcgttcc
     4501 cgcactatac tgattcaaaa ccgggtggag agacgcgtgg acaggctgct caaggtgctg
     4561 atcgccgatg attcggaaga tgatgcactg ctgttggttc ggcaactaaa aaagggggga
     4621 ttgacctgct ggttcgaacg ggtggacacg gcggaggaca tgcgcgtttc actcaaccgc
     4681 cagcactggg atctggtggt aaccgaccac aacatgcctg ctttcagttc tgccgaagcc
     4741 ctgcagctgg tcaaggccat ccagcctgac acccccgtga tcgtggtgtc gggcgccatc
     4801 accgacgatg tggcggtgaa ctcgatgaag gccggcgcgc aggattacat catgaaggac
     4861 aatctggcgc gactggtgcc ggcggtggag cgcgaattgc gtgacgccga gcatcgcaat
     4921 gcacgcaagg aagcggagca gcaggtcgcc tacctgatct atcacgactc cctcaccgat
     4981 ctgatgaacc gcgaccagtt caagaagcag ctggagacgg tgctggagaa tgctcgcgta
     5041 cacggccacg aacatgcgct gctgtatgtg gacctggacc agttcaagat catcaatgac
     5101 acctgcggcc atgtggccgg cgatgaactg ctcaagcaga tcggcaaggt gatcaagggc
     5161 gagctgcgcg agcaggatcg cgtgtcccgc atgggcggcg atgaattcgg catcctgctg
     5221 tggcagtgca atctggatga agccctggag gcggcgcgca aggtacacaa cgccatcaag
     5281 gaattccggt ttctgtggaa tgaacaacgt ttcgtgattt catccagcat cggtgtggtg
     5341 cagctcaaca gtgcatccac cgatgtagaa gaagtgctgg ccagcgccga catggcctgc
     5401 tatgccgcca aggacaaggg ccgcagttac atccaggtgt tcgatgccca cgaccgtgaa
     5461 ctgacccagc gccgcgacca gatgcactgg gcttcgcgaa tccgccgcgc actggatgaa
     5521 aacgcgttcg tgctgtattc ccaggccatc gtggggttgg cacaaccagc ccggcacgaa
     5581 gagttcctgg tgcgcatgcg tgatggcgat accattattg ctcccggtat gttcatgccg
     5641 gcggcagaac gcttcaacct gatgccgctg atcgaccgct gggtgatccg caatgctttc
     5701 gcccatgtta aaaagcggct ggagcagaac cggctggcgg atggcgatct gttcttcatc
     5761 aatctgtccg gctcgtccct gagcgatgaa tccttttttg aattcgtgca ccagtgccgg
     5821 gaagaattcg aactgcccca gcgcaagatc tgtttcgaaa tcaccgaaac cactgccata
     5881 tccaatctgg aaaccgcgtc gcagttcatt tccgaaatcc gcgatctggg ctttctgttt
     5941 gcactggatg attttggcgt gggcatgagt tcgttcgcct atctgaaggc aattccggtg
     6001 gacgtgctga agatcgacgg cggctttgtg cgcaatattc tcaacgatcc catggatcag
     6061 gccattgttg aagcctgcaa caatatcgcc cattccgcgg gtctgatcac cgtggcagag
     6121 ttcgtcgaga acaaggccat cgaggatcgt ctgcgtgaac tcggggtgga tttcgggcag
     6181 ggctatggta tcgaaaagcc ccgtcccatc gatgcctgaa acgaccactg cccggacgct
     6241 ggtgttcgcg ctggccagcc tgctggcggt gtccgcgcag gctgatacct attccagtcc
     6301 cccgcattcc ggatcgtctc ttgccgtggc tccacagccg gtgtcggatg ctgcgccgct
     6361 ggtggcgtat gcgccggatt tcctgcccca ggaatggccc tggatcgata ccagcgtgga
     6421 gctggaggat ctgccggaag aggaggagct gccggaagcg gaaccggaag gttacatgga
     6481 tcgcagcgtc gattatctgc tggagcagcg caaatccatt tccacccggg tgaacgtgat
     6541 ggcccgcgcc atcgaccgct atctggccgg cgatcgcgcc atggccgagg aaaatgaaac
     6601 cttcatgaaa ttacagctgg cggagcgctg gattgaaggt ggcaagtggg atccggacaa
     6661 cgatctgaaa ttccgccttg atctgccggc caccaagcgg cgctaccggc tggtac
//
LOCUS       DASUEG010000081         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1895633, whole genome shotgun sequence.
ACCESSION   DASUEG010000081 DASUEG010000000
VERSION     DASUEG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895633"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VFX11_01345"
     CDS             complement(<1..2154)
                     /locus_tag="VFX11_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004588883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4937292.1"
                     /translation="MLNAIIDLGVKNRLLVLLSLIALIVGSVLILPRLNLDAFPDVTN
                     VQVQINTEAQGLAAEEVEQLITFPVEAVMYALPDVEAVRSISKTGLSVITVVFKEGTD
                     IYFARQLVFERLQAAREEIPDGIGTPEMGPNTSGLGQVYQYILRSERPQQYDAMALRS
                     LNDWVVKMLLMPVAGVTEVLSYGGEVKQYQVNVDPSRLLAHDLTIDEVVEAIETNNRN
                     AGGWYLDRGAEQLVIRGVGWVRSDSAGLEDIGNIPLKNIKGVSVKVSDVANIGFGSEI
                     RQGALTMTRRNASGEPEALGEVVAGIVLKRMGANTKATIEGVKARLPAIQAALPDGVT
                     IEPFYDQAELVDKAVETVTKALLEAFVLIVVVLLLFLMNLRAALLVLISVPVSIGLAL
                     MAMAYWGISANLMSLGGLAIAIGMMVDGSVVMMEHIFSHLTHPDREHDPQSGRLTSPE
                     DADRHSVESDDHGIGLRIQEAAKEVGRPVFFAVMIIIIVFAPLFSLQGVEGKLFQPMA
                     MSIVLAMLASLLVALLMVPALATYLFRRGVKHRDNVLTRTLDRGYRRLLQSALKQRSL
                     VTGSALAMLVGALFLVPFLGTEFVPELEEGTLNIRVTLAPSSSLDTSVEVAQKMEQVL
                     MTFPEVLYAASRVGRPELGGDPEPVSNVELYVGLKPVEEWTSAPDRRALQALMQERMS
                     IHPGLLFSFSQPIATRVDELLSGVKAQLAVKLFGPD"
     gene            complement(2166..>2796)
                     /locus_tag="VFX11_01350"
     CDS             complement(2166..>2796)
                     /locus_tag="VFX11_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4937293.1"
                     /translation="LSESDIQALESGAKVKLGEYTLRAVAAGVVLSDDFHQGQRVDAG
                     ETIMDLADETELWVEARLPPGTALSIPVGTPALIKTGGESFAARVTQEAHKIDPRSRT
                     RIVRLVVDNAQHKLHSGLFADVAFQFTTPEPVLALPESAVLRGADGDWQVFVETAQGR
                     YEPREVELVQTLGEHLVISGVDSGSRVVTSGAFFVQSEIAKSGFEVHNH"
BASE COUNT          627 a          881 c          728 g          560 t
ORIGIN      
        1 atcagggcca aacagtttca ccgccagctg ggctttgacg ccggacagca gttcatccac
       61 gcgggtcgca atcggttggg agaaagagaa caacaaaccc ggatgaatcg acatacgttc
      121 ctgcatcaat gcctgcaatg cgcgccggtc aggtgccgag gtccattcct caacgggttt
      181 gaggcccacg tacagttcca cgttggaaac cggctcgggg tcgccgccca gctccggtcg
      241 ccccacgcgg ctcgcagcgt acaacacttc cggaaacgtc atcagcacct gttccatttt
      301 ctgcgcgact tccaccgagg tatccagact ggaagacggt gccagggtga cgcgaatgtt
      361 gagcgtgcct tcctccagct cgggcacgaa ttcagttccc agaaacggaa ccaaaaacaa
      421 cgcgcccacc aacatggcca gcgcacttcc cgttaccaat gaacgctgtt tcagcgcact
      481 ttgcagcaga cgccggtaac cacggtccag tgttcgggtc agcacgttgt cccggtgttt
      541 gactccgcga cggaacagat aggttgccag ggcgggcacc atcaacaacg ccaccagtaa
      601 cgaagcgagc atcgccagca cgatggacat ggccatgggc tgaaacaatt tgccttctac
      661 cccttgcaga ctgaacagtg gcgcgaacac gataataatg atcatgacgg cgaaaaaaac
      721 cgggcggccg acttcctttg cggcttcctg aatacgcaac ccaataccgt gatcatcgga
      781 ttcaacgcta tgccgatccg catcctctgg cgaggtgaga cgtccggact gggggtcatg
      841 ttcgcgatca gggtgcgtca gatgactgaa gatatgctcc atcatcacca ctgaaccatc
      901 caccatcatg ccgatggcga tcgccagccc acccagggac atcaggttgg cggaaatacc
      961 ccagtacgcc atggccatca atgccagccc gatcgatacc ggcacggaaa tcagcaccag
     1021 caacgccgcg cgcagattca tcagaaacag cagcaatacc acgacgatca gcacgaaggc
     1081 ttccagcaaa gctttcgtca ccgtttccac cgctttgtcg accaactctg cctgatcata
     1141 aaaaggctcg atggtaacgc cgtccggcaa tgcggcctga atggcgggca agcgtgcttt
     1201 gacgccttcg atggtggctt tggtgttggc gcccatgcgc ttgagtacga ttcccgccac
     1261 cacctcgccc aatgcttctg gctcgccact ggcattgcgt cgcgtcatgg tgagcgcgcc
     1321 ctggcgaatt tcagaaccaa atccgatatt ggccacgtca ctgactttca ccgacacgcc
     1381 cttgatattt ttgagcggga tattaccaat gtcttccagt ccggcactgt cgctgcgtac
     1441 ccagcccacg ccccggatca ccagctgttc ggcgccacga tccagatacc aaccaccagc
     1501 gttgcgattg ttggtttcaa tcgcttccac cacttcatcg atggtcaggt catgggccag
     1561 cagccgggag ggatcgacat tgacctgata ctgtttgacc tcgccaccgt atgacagcac
     1621 ttcggtaacg cctgccaccg gcattagcag catcttcacc acccaatcgt tcaggctgcg
     1681 caaggccatc gcatcatatt gctgtggacg ctcgctgcgc aggatgtact ggtaaacctg
     1741 acccagcccg gacgtattcg gccccatctc cggcgtgccg ataccatcgg gaatttcttc
     1801 cctggcggcc tgtagccgct caaaaaccag ctggcgtgcg aaatagatat cggtgccttc
     1861 cttgaacacc acagtgatga cggacaatcc ggttttggaa atggagcgca cagcctccac
     1921 atccggcagt gcatacatga ctgcctcgac gggaaaggta atcagctgtt caacttcttc
     1981 ggccgccagt ccctgcgctt cggtattaat ctgaacctgt acgttggtga catcagggaa
     2041 cgcatccaga ttgagacgcg gcaggatgag cacactgccc acgatcaggg caatgagcga
     2101 caacaagacc agcaggcggt ttttgacgcc caggtcgata atagcattca gcatcgcacg
     2161 gtccctcagt ggttgtggac ttcaaaaccg cttttggcga tttcagactg cacgaagaaa
     2221 gccccgctgg taaccacccg gcttccactg tcaacaccgc ttattaccag gtgctcgccc
     2281 agtgtctgca ccagttccac ttcacggggc tcatatcgac cctgcgccgt ttccacaaac
     2341 acctgccagt cgccgtcggc accacgcagg acagcagatt caggcaacgc cagaaccggt
     2401 tcgggcgttg tgaactgaaa agccacgtcg gcaaacagtc cggaatgcag tttgtgctgc
     2461 gcattgtcca ccaccagacg cacaatgcgg gtccggctgc ggggatcgat tttgtgtgct
     2521 tcctgcgtaa ctcgggcggc aaagctttct ccgccggttt tgatcagggc aggcgtgccc
     2581 acgggaatac tgagcgcggt gcctggtggc aaccgtgctt ccacccagag ttcggtttca
     2641 tccgccagat ccatgatggt ttcaccggca tccacccgtt gcccctgatg aaaatcgtcg
     2701 gacaacacca cgccagccgc cactgcgcgc aaggtgtatt cacctagctt caccttggcg
     2761 ccggactcca gcgcttggat atcggattca gacaac
//
LOCUS       DASUEG010000082         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_371881, whole genome shotgun sequence.
ACCESSION   DASUEG010000082 DASUEG010000000
VERSION     DASUEG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371881"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /gene="glgP"
                     /locus_tag="VFX11_01355"
     CDS             complement(<1..1162)
                     /gene="glgP"
                     /locus_tag="VFX11_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX4937294.1"
                     /translation="MAGTRYTIEVQPKIPEALNRLDELANNLLYSWDRQVRGLFFRMD
                     RELWESTGHNPKMFLRRIDQRKLEQAADDPVYMQEYAKVISTYDTYLKTRAKLKAVKS
                     INTEQDLISYSCAEFGLHESLPIYSGGLGILAGDHCKAASDIGLPFAAVGMLYRQGYF
                     TQQIDAHGNQIAQYNPSRFSDLPVKPATGANGECVEVSIEVPGRVIRLQVWTARVGHI
                     TLLLLDSDLEKNSEEDRRITYQLYGGDITNRLLQEMVLGIGGIRAKRAMGLKPTVFHI
                     NEGHAAFQVLERCRELVKEGLDFYSALEAVASNTVFTTHTPVPAGHDIFDHRLIEFYF
                     SQYVTELKISMSEFLALGASPVNDHGFNMTALALRGSRFHNGVSRIHGGVASE"
     gene            1384..2346
                     /locus_tag="VFX11_01360"
     CDS             1384..2346
                     /locus_tag="VFX11_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX4937295.1"
                     /translation="MPLLAGDIGGTKTLLGLQSAHGALEQVREYRNQAHDGVDAILHD
                     FLQQTGTRPDDGLHLVLAIAGPVDQARCQMTNLPWLIDSATLQQQFRFKSVHLLNDLE
                     ATGWAMPGLGAAGLLLPLRGDHIDFGRPVAVMSIGTGLGQAALIPQKGGTLQVLSSEG
                     GHKQFAPFDARSASLLQQAYATGHTGVSWENWFSGSGLPRLFQALFPDMEAPDSQTIT
                     ELAVAQPGSCEAQCVHLLMQGLMAEAGNLALQYAAWGGVIFAGGVAQHLLPYWHDPDL
                     QRWLEHKTDHVQRLRQVPLAMCTDTQAALRGAADYFWHQQMRGN"
     gene            complement(2325..3359)
                     /gene="hypE"
                     /locus_tag="VFX11_01365"
     CDS             complement(2325..3359)
                     /gene="hypE"
                     /locus_tag="VFX11_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX4937296.1"
                     /translation="MKDARILLTHGSGGESTRDLIRQLFLPGYGNSQLNLLTDGAVLT
                     SATAQQVFTTDSYVVNPPFFPGGDIGALAVIGTCNDLAVMGAVPRWLSLSFILEESLP
                     IERLEKIVQSIQRTAAEAGISIVTGDTKVVERGKADRLFINTAGIGELHIPQALTPTR
                     IQPGDAILVSNDIGRHGIAVLASREGINLDVDLLSDMALLHPLIQALLNQGIDLHCVR
                     DLTRGGLGGAMLELAEQCGQNFRVQETDVPVSDTVKAACELLGFDPLFIANEGCCALF
                     LPQSQVADALDILREFSAGRWSARIGTVLHGSATQPVAGKSGGVFLENAYGVERLLAW
                     HTVDQLPRIC"
BASE COUNT          728 a         1090 c         1068 g          696 t
ORIGIN      
        1 tttccgacgc gacaccgccg tgaatccggc tcacaccgtt gtggaagcgg gaaccgcgca
       61 gggccagcgc cgtcatgttg aagccgtggt cgttgaccgg gctggcgccc agcgccagga
      121 attcgctcat gctgatcttc agctcggtaa cgtactggga aaaatagaac tcgatcagac
      181 ggtgatcgaa aatatcgtgc cccgccggca ccggtgtgtg ggtggtgaac acggtattgg
      241 acgccaccgc ttccagcgcg ctgtagaaat ccagcccctc cttcaccagt tcccggcagc
      301 gttccagcac ctgaaaagca gcgtggcctt cgttgatgtg gaatacagta ggcttcaggc
      361 ccatggcgcg cttggcccgt atgccgccaa tgcccagcac catttcctgc aacagccggt
      421 tggtaatgtc gccaccatag agctgatagg tgatgcggcg atcctcctcg ctgttttttt
      481 ccagatcgct gtccagcagc agcagggtga tgtgccccac ccgcgccgtc cacacctgca
      541 ggcgaatgac gcggcccggc acctcgatgc tgacttcgac acactcgccg ttggcaccgg
      601 tggccggctt cactggcaga tcgctgaaac gcgaaggatt gtactgggca atctggttgc
      661 catgagcgtc gatctgctgg gtgaagtaac cctgccggta caacatgccc accgcggcaa
      721 atggcaggcc gatgtcactg gcggccttgc agtggtcacc cgccagaatg ccaagcccgc
      781 ccgagtagat cggcagactt tcgtgcaatc cgaattcggc acaggaatag gaaatcagat
      841 cctgctccgt attgatgctt ttcaccgcct tgagtttggc ccgggtcttc agataggtgt
      901 cgtaggtgga gatgaccttg gcgtactcct gcatgtacac cggatcgtcc gctgcctgtt
      961 ccagcttgcg ctggtcaata cggcgcagga acattttcgg attgtggccc gtgctttccc
     1021 acagttcgcg atccatgcgg aagaacaggc cgcgcacctg gcgatcccag ctgtacagca
     1081 ggttgttggc aagttcgtcc aggcggttca gggcttcggg aatcttgggt tgaacttcaa
     1141 tggtgtatcg ggtaccggcc atggagatgt cctgatgcaa atgggtggtt cagtgtaatc
     1201 acaagattag atcaaacggg aatccgcacc gcttgattgc cgtcaatcgc ttggtggaag
     1261 gtccgggtca gaatacactt attcggacgg caccaaaacc cgatgcaaaa aacggtgcag
     1321 tggacgctgt gtcctttgcc agggacagca aaccggggcc acccaacaaa ggagggacgc
     1381 aggatgccat tactggcggg cgatatcggc ggaaccaaaa ccctgctggg tctgcaatcg
     1441 gcgcacggcg cgttggagca ggtacgggaa taccgcaatc aggcccatga cggggtggac
     1501 gccatcctgc atgacttcct gcagcaaacg ggcacccgtc ccgatgacgg actgcatctt
     1561 gttctggcca tcgctggccc ggtggatcag gcccgctgcc agatgaccaa cctgccctgg
     1621 ctgatcgaca gtgccaccct gcaacagcaa ttccgcttta aatcagtgca tcttctgaac
     1681 gacctggaag ctaccggctg ggcgatgccc ggcctgggtg ccgcaggcct gctgctgcca
     1741 ctgcggggag atcacatcga ttttggccgc ccggtcgcgg taatgagcat aggcacgggg
     1801 ttgggacagg cggcgctgat cccgcagaaa ggcggcactt tgcaggtgct gtcatcagaa
     1861 ggcggtcaca agcagttcgc tccattcgac gcccgcagcg ccagtctgct gcaacaggcc
     1921 tatgccaccg gccacaccgg cgtgagctgg gagaactggt tttccggatc gggtctgcca
     1981 cggctgttcc aggcgctgtt tccggacatg gaggcccccg acagccagac catcaccgaa
     2041 ctggcggtgg cacagcccgg ttcctgcgag gcgcaatgcg ttcacctgct gatgcaggga
     2101 ttgatggccg aggccggcaa cctggcgttg cagtatgcag cctggggcgg cgtgattttt
     2161 gccggcggcg tggcccagca tctgctgcca tactggcacg atcccgatct gcaacgctgg
     2221 ctcgaacaca agaccgatca cgtgcaacgg ctgcggcagg tgccactggc aatgtgcacc
     2281 gacacgcagg ctgcgttacg aggggctgcg gattacttct ggcatcagca gatgcgcggc
     2341 aactgatcca ctgtgtgcca tgccagcagg cgctcgacac cgtaggcatt ttccagaaac
     2401 acacctccgc tttttccggc gactggttgg gtcgcgctgc cgtgcagaac agttccgatt
     2461 cgggccgacc agcgcccggc agaaaattcc cgcagaatat ccagcgcatc cgccacctgc
     2521 gattgcggca ggaacaatgc gcaacaccct tcgttggcga tgaacagcgg atcgaaaccc
     2581 agcagctcgc acgccgcttt cactgtatcc gacaccggca catccgtttc ctgcacacgg
     2641 aaattctgcc cacactgctc tgccagttcc agcattgccc cgcccagtcc gccccgggtg
     2701 agatcacgga cacaatgcag atcaatgccc tgattgagca aggcctggat cagcggatgc
     2761 aatagcgcca tatccgacag cagatccacg tccagattga tgccttcgcg actggccagt
     2821 accgcaatgc catgacggcc gatgtcgttg ctcaccagaa tcgcgtcgcc gggctggatg
     2881 cgcgtgggcg tgagcgcctg cggtatatgc agttcaccga tgccggcagt attgatgaaa
     2941 agccggtccg ccttgccccg ttccaccacc ttggtgtcac cggtgacaat ggagatgccc
     3001 gcttcagcag ccgtgcgctg gatcgactgc acgatttttt ccagccgttc gatgggcagc
     3061 gattcttcca gaatgaagga cagcgacaac cagcgcggca cagcgcccat caccgccaga
     3121 tcattgcagg tgccgatcac cgccaaggcg ccaatgtcgc cgccgggaaa aaacggcgga
     3181 ttgacgacat agctgtcggt ggtgaacacc tgctgggcag ttgcgctggt caacaccgca
     3241 ccgtcggtga gcaggttcaa ttgcgaattg ccgtaacccg gcagaaacag ctgccggatc
     3301 aaatcgcggg tggattcgcc accgctgccg tgagtcagca ggatgcgcgc gtctttcatg
     3361 gctgcaggtc cgtttttaag ttgtcttgcg tattttgcgc tggaaatacc gggcggagac
     3421 ggcttaatga caagaatcgg gatacaccac ttgccccagc gccagtccgc cgtcgttgag
     3481 gggcaaggtc gcgggccaca tcaggtgcac cgccattggc ttcgcccgat ccagcagcaa
     3541 ggattgcagt agccggttct gaaagcaacc tccactcagg ga
//
LOCUS       DASUEG010000083         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_40538, whole genome shotgun sequence.
ACCESSION   DASUEG010000083 DASUEG010000000
VERSION     DASUEG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40538"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VFX11_01370"
     CDS             <1..1496
                     /locus_tag="VFX11_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX4937297.1"
                     /translation="DRITRLFLSFICAGALTGTAEAAPWIDPGDEQLRHHIEVLADAG
                     VIRSPITTWPIMWGAVSRDLEQAKDQSLDDATLWSLAYVRFALNRATEALNLTAYAGG
                     RSDAAAIADFANDQREQNEARGEADWVGEHLAARLRLAWVPDATDGKDYRYDGSYVAG
                     LLGNWSLSAGAIDRWWGPGWQSSLNLSTSARPVNSVQLQRNFSDPFETRWLSWIGPWT
                     LTLFAGQLESESAVPDAKLLGARLAFRPFSSLELGFARNAQWGGEGRPQDLDSLWDLV
                     VGNDNGDDDGYDPVVDPANDPSNQLGAVDLRWSFPLLQTRSAFYLQYTGEDESNGFPS
                     RGMDLAGIETAFASRNLFHRFALEYVNSMAGAHTDPRPNTAYEHSVYLSGYRYRSRPI
                     GASFDNDTRALTLMGDHYFTDGDQISWRIIQVALNRDGENRNNGWGGSVFAAEAVDTL
                     LLQASYSIVIKNFKISTSVYNVDEEIMWNDESINGTGAGLAVEYRFQ"
     gene            1540..3981
                     /locus_tag="VFX11_01375"
     CDS             1540..3981
                     /locus_tag="VFX11_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX4937298.1"
                     /translation="MLWGWVGGAYAFTPTPEQIEQFRQLPREEQERLARQYGVDLNQL
                     MPAAGVQAPAMPADTPNQRKMRVQPDSRTDDQNVVTFPTEDAQEPEQVEATDAETALK
                     PYGYDLFSNNATAFQPNVDIPVPADYVVGPGDTFVVQLYGKDNASHSLSVSREGVIQL
                     PEIGPIALAGLRFSEAQGVIERTISEQMIGVKCSVTMGTLRTIRVFVLGEAVQPGSYI
                     VGSLSTMTNALFASGGVTPIGSLRSIQLKRQGEIVTTLDLYDLLLNGDTRDDARLLPG
                     DVIFIPPVGATVGVSGEVRRPALYEIKHEKSAADLVRLAGGFMPTAYPPASRIERIST
                     QGERTVVNIDLARAEGKNLAVKTGDVLRIFSVLDTMENIVTVEGHVKRPGINAWKPGA
                     RVSTVLGSIRELLPNPDLNAAIIERELPPTGRLKVISFNLGEALTHPGSAADISLQPR
                     DKIRVFDLEKDRSKLLEPVIGNLRMQASRENRQKVVSISGNVRFPGNYPLTDSMSAQQ
                     LIALAGGLSENAYNLEGEITRYFLDAEQHQQIEYVDLKLASATQYKLQEEDRLNIRRL
                     PDWSPEETIELSGEIRFPGKYTIRRGETLSQILQRAGGLTNEAYPEGAVFTRKDLQVL
                     ESQRLQELKQQLESDIAAANIEQQDDKGKVDVKDAEQLLKNIEGVRPLGRMVIDLPLL
                     VSKPEQQDVRILNGDKLHVPRYKQSVTVVGEVQFPTSHLYEAKLDVQDYIERSGGTNL
                     KADKARVYVVKANGRVFLPQKSGWFSRDMTSVEPGDTVVVPLDADRIKSLTLWTSVSQ
                     VFYQIALGAAAVASF"
BASE COUNT          890 a         1105 c         1221 g          853 t
ORIGIN      
        1 cggatcgaat cacacggctt tttttgtctt ttatctgcgc aggcgcgctg accggcactg
       61 cggaggctgc cccctggatc gatccagggg atgaacagct gcgtcatcac atcgaagtgc
      121 tggcggatgc tggcgtcatc cggtccccca ttaccacctg gcccatcatg tggggtgcgg
      181 tgtcgcggga tcttgagcag gcgaaagacc agtctctcga cgatgccaca ctctggtccc
      241 tggcctatgt gcgctttgcc ctaaaccgtg ccactgaagc gttgaacctg accgcgtacg
      301 cgggcggacg cagtgatgct gccgccattg ccgacttcgc caatgaccag cgcgagcaga
      361 atgaagcacg gggcgaggcg gactgggtgg gtgaacatct ggcagcgcgg ctgcggctgg
      421 cctgggtgcc ggatgccacc gacggcaagg attaccgcta cgacggcagc tacgtggccg
      481 gcctgctggg aaactggtcg ctcagtgccg gcgccattga tcgctggtgg gggccgggct
      541 ggcagagcag cctcaatctg tccaccagtg cgcgcccggt caattccgtg cagctgcaac
      601 gcaatttttc cgatccgttc gaaacccgct ggctgagctg gattggcccc tggacgctca
      661 ccctgtttgc cggtcagctg gaaagcgaga gcgcggtgcc ggatgccaaa ctgctgggtg
      721 cgcggctggc cttcaggccg ttttcatcgc tggagctggg ttttgcgcgc aatgcgcagt
      781 ggggtggcga ggggcggccg caggatctgg acagtttgtg ggatctcgtc gtgggcaatg
      841 acaacggcga tgacgatggc tacgatccgg ttgtcgatcc cgccaacgat cccagcaatc
      901 agctgggtgc tgtcgatttg cgctggagtt ttccgttgct gcaaacccgc tccgcatttt
      961 atctgcaata caccggtgaa gacgaaagca acggctttcc ctcgcggggt atggatctgg
     1021 ccggcattga aactgcgttc gcttcgcgca atctttttca ccgtttcgca ctggaatatg
     1081 tgaattcaat ggccggcgcc cacactgacc cgcgccccaa caccgcttat gaacacagcg
     1141 tttatctttc aggttatcgt tatcgttcgc gtcccatcgg tgcatctttc gacaatgaca
     1201 cgcgcgcact tacgcttatg ggtgatcatt atttcaccga tggtgatcag atttcctggc
     1261 gcatcatcca ggttgcactg aatcgcgatg gtgaaaatcg taacaacggc tggggtggca
     1321 gcgtatttgc cgccgaggcc gtcgacacgc tcctgcttca ggcgtcgtat tcaatcgtta
     1381 taaaaaactt caaaatctcc acgtctgtct ataatgttga cgaggaaata atgtggaacg
     1441 atgaatcgat caatggaaca ggggcggggc tggcagttga atacagattc caataactcc
     1501 aataaaaaaa ctgctcgaat catactgact gttgttttga tgttgtgggg atgggttggc
     1561 ggagcctatg cgttcacgcc cacacccgag cagatcgaac aattccggca gctgccacgg
     1621 gaagagcagg agcggctggc gcggcagtat ggtgtggatc tcaaccagct gatgccggca
     1681 gcgggtgtgc aagcgcctgc aatgccagcc gatacaccga accaacgtaa aatgcgggtt
     1741 cagccggata gccggacaga tgaccagaat gtcgtgacgt tccctaccga agacgctcag
     1801 gaacctgagc aagtcgaagc aacggacgcc gaaaccgcgc tcaagcccta cggctacgat
     1861 ctcttcagca ataacgccac tgcctttcag cccaatgtcg atattccagt gcccgccgat
     1921 tacgtggtgg ggccgggcga cacgtttgtc gtgcagctgt atggcaagga taacgccagc
     1981 cattccctca gcgtgagccg cgaaggcgtg atccagttgc cggaaatcgg ccccatcgca
     2041 ttggccgggc ttcggttcag cgaagcgcag ggcgtcattg aacgtaccat ttccgaacag
     2101 atgatcggtg tgaaatgctc tgtcaccatg ggcacactgc gtaccatccg cgtatttgtg
     2161 ctgggcgagg cggtgcagcc tggttcctat attgtgggtt cgctgtccac catgaccaac
     2221 gcactgttcg ccagcggtgg cgtcacgccg atcggttcgc tgcgttccat tcagctgaaa
     2281 cgtcagggcg aaatcgtcac gacactggat ctttatgatc tgctgctgaa cggcgacacg
     2341 cgcgacgatg cacgcctgct gccgggcgat gtgattttca ttccgcccgt gggcgccacg
     2401 gtgggcgtca gcggtgaagt gcggcgtcct gcactgtatg aaatcaaaca cgaaaagagc
     2461 gcggcggatc tggttcgtct ggcgggcgga ttcatgccca cggcgtatcc accggcgtcg
     2521 cggatcgagc gcatcagtac ccagggcgag cgcaccgtag tgaatatcga tctggcgcgt
     2581 gctgaaggca agaacctggc ggtgaagacc ggcgatgtgt tgcgcatttt ctcggtgctc
     2641 gacaccatgg aaaacatcgt aacggtggaa gggcatgtca aacgcccggg gatcaacgcc
     2701 tggaaacccg gtgcacgcgt aagcacggtg ctgggaagca ttcgcgaact gttgcccaat
     2761 cctgatctga atgccgccat catcgagcgc gaacttccgc ccacaggccg cctgaaagtc
     2821 atcagcttca atctgggcga agcactcacc catcccggca gtgccgctga catcagtctg
     2881 cagccacggg ataaaatcag agtattcgat ctggaaaagg atcgcagcaa actgctggaa
     2941 ccggtgattg gaaatctgcg catgcaggcc agccgcgaaa accggcagaa agtggtgagc
     3001 atcagcggca acgtgcgatt ccccggcaac tatccactga ccgacagcat gtcggcccag
     3061 caattgattg cattggcggg cggattgagt gagaacgcct ataatctgga aggggagatc
     3121 acccgctatt tcctggatgc cgagcagcat cagcagatcg agtatgtcga tctgaagctg
     3181 gcgtccgcga cacaatataa attgcaggaa gaagaccggc tcaacattcg ccgactgcct
     3241 gactggtcgc cggaagagac tattgaactg agtggtgaaa ttcggtttcc gggcaaatat
     3301 accattcgcc gtggcgaaac cttgtcgcag attctgcagc gggctggcgg cctgaccaat
     3361 gaagcctatc ctgaaggggc tgtctttacc cgcaaggatc tgcaggtgct ggaatcgcaa
     3421 cggttgcagg aactgaagca gcagctggaa agcgacattg ctgcggcgaa tatcgagcag
     3481 caggatgaca agggaaaagt cgatgttaaa gatgctgaac aattgctgaa aaatatcgaa
     3541 ggtgtgcgac cgttgggccg catggtgatt gatctgcctt tgctcgttag caagcccgag
     3601 caacaggatg ttcgcattct gaatggcgac aaactgcatg ttccgcgcta caagcagtcc
     3661 gttactgtcg tgggtgaggt gcagtttcca acatcgcact tgtatgaggc aaagctggat
     3721 gtgcaggatt acatcgagcg ttctggcggc accaatctga aggctgacaa ggcgcgtgta
     3781 tatgtcgtga aagccaatgg tcgtgtattc ctgccgcaga aatccggctg gttctcgcgc
     3841 gatatgacaa gcgtggaacc cggtgacacg gtagtggtgc cgctggacgc cgatcgcatc
     3901 aagtcactca ccctgtggac cagcgtgtcg caggtttttt accagattgc actgggcgcc
     3961 gctgcagtgg cgagtttcta atatttatgg gaggttgcag taaacacaca atgatgtttt
     4021 aagcgtagaa aagacgtcaa tccatgggta tcgaagagca acgcccggc
//
LOCUS       DASUEG010000084         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1453964, whole genome shotgun sequence.
ACCESSION   DASUEG010000084 DASUEG010000000
VERSION     DASUEG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453964"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..795)
                     /gene="rsuA"
                     /locus_tag="VFX11_01380"
     CDS             complement(97..795)
                     /gene="rsuA"
                     /locus_tag="VFX11_01380"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004395387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HEX4937299.1"
                     /translation="MRLDKYLSNFSGLSRKEARQVIKAGDVLVNGVMLTDPTCSIADD
                     AQVQWSGLHIAPTGPRYFMLNKPVGYVCANSDSHNPTVFELLDEDALEDLQVAGRLDI
                     DTTGLVLITDDGQWNHRVTSPKTQKSKCYDVQLAEPVTADAIPLFAEGILLRSEKKRT
                     RPAQLDIISPTQVRLTIHEGKYHQVKRMFAAIGNQVVALHRDSIADIVLDPNLAPGEY
                     RPLTAAEIASIDDV"
     gene            1104..1250
                     /locus_tag="VFX11_01385"
     CDS             1104..1250
                     /locus_tag="VFX11_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937300.1"
                     /translation="MSSVRILDWLGSAALTIAIVSLLFLFLPGIVRAESGMAGNAGVE
                     FCSE"
     gene            complement(1466..>2285)
                     /locus_tag="VFX11_01390"
     CDS             complement(1466..>2285)
                     /locus_tag="VFX11_01390"
                     /inference="COORDINATES: protein motif:HMM:NF038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor H family protein"
                     /protein_id="HEX4937301.1"
                     /translation="DDGDGYYHQFNINFDIDTRLSSASIYVTGQLNNGTSTPLFRTDP
                     FTIRGATGSDSYQATVLLTDGYPATRYELTLRVYDASSNALLFTYGPQQDSDLAQLYL
                     EDVTREAVSSGDLDLFVLEFTLHGDSDGDGYYTRADVRFDADAPGRTATLYASLYLID
                     QRGEWIPLRDTGTFTASHYSHSDAVNFGFDLNSGFDPQRYRLGLEIRDAYSHMVLLTS
                     VTPDATPLHMESVDYDASYTVVEEHHDDGHSGGSLHWLLLGGLTLLLARRLRRF"
BASE COUNT          433 a          591 c          739 g          522 t
ORIGIN      
        1 tgcagcaggg cggagcagtc cagatgctca tcggcgatca gcagcttgcg gccggaggcg
       61 ttgcgcagcc gctcgaacag ggcgtgcagg gcaggatcag acatcgtcga tactcgcaat
      121 ttcagcggct gtcagtggcc ggtattcacc gggcgccagg ttagggtcca gcacaatgtc
      181 cgcaatgctg tcccggtgca acgccaccac ctgattgcca atggcggcga acatgcgctt
      241 cacctgatgg tatttgcctt cgtgaatggt gagtcgcacc tgcgtggggg aaatgatgtc
      301 cagctgtgcc ggacgggtgc gttttttttc gctgcgcaac aggatgccct cggcgaacaa
      361 gggtattgca tcggccgtga ccggttctgc cagctgcacg tcatagcatt tggatttttg
      421 ggttttcggc gacgtgacac ggtgattcca ctggccgtca tcggtgatca acaccaggcc
      481 ggtggtgtcg atgtccaggc gacccgccac ctgcaggtct tccagcgcat cttcatccag
      541 cagttcgaac acggtggggt tgtggctgtc gctgttggcg cacacatagc ccaccggttt
      601 gttgagcatg aaatagcgcg ggcccgtcgg ggcgatgtgc aggccggacc attgcacctg
      661 ggcgtcgtcg gcaatgctgc aggtgggatc ggtcagcatg acgccgttta ccagcacgtc
      721 gccagccttg atcacctgtc gcgcttcctt gcgggacaat ccggaaaaat tgcttaggta
      781 cttatcgagt cgcatgaggg gccgtgtcga aaaacggtga ttatacgggg cttcgcgtgc
      841 caataaaacc aatcggtctg cgcggtggga tcgggccggt tggctgggtt ttagattgat
      901 tggttacatg ctgtcgggcg tcctagaatg tgatgccatt aatcattttt tgaaaattca
      961 atcataacag gcggcttgct gcattagttc aactgactgc tcagctttgc cggtcaatgg
     1021 cggcgacagt gtcgccttgc ggctctgacg gtgaccatta cactgacttt atcgtgcggt
     1081 tttgagcaat aagggagtgt gccatgagca gtgtcaggat acttgattgg ctgggttctg
     1141 ccgcgttgac cattgccatt gtgtcattgc tgtttttgtt tttgccgggc attgtgcgag
     1201 cggaatctgg catggccggt aatgctggcg tcgaattctg tagtgagtga tgtgaagtcg
     1261 cggcctgttg aaaccgcaga agaagtgcaa ggagtgaatc agtattgaat gatggtgaaa
     1321 cgatttggca gtgccgacct gtaagaccgg agtgaaagac agcggcaatc agcaggcagt
     1381 cggttgaccc ggggtagcaa gccacagttc tcacgaactg tggctttttc gttggcgcaa
     1441 ctttatatag ttatttcggt gcgaatcaaa accggcgcag gcggcgcgcc agcagcaggg
     1501 tcagtcctcc cagcagcagc cagtgcagcg atccgcccga gtggccgtca tcgtgatgct
     1561 cctccacgac cgtgtagctg gcgtcatagt ccacgctttc catgtgcagg ggagtggcat
     1621 ccggtgtgac cgatgtcagc agtaccatgt gactgtaggc atcacggatt tccagcccca
     1681 gccggtagcg ttgcggatca aagccgctgt tcagatcgaa gccgaaattt accgcgtcgc
     1741 tgtgggaata gtggctcgcg gtgaaggtgc cggtgtcgcg caggggaatc cactcgccgc
     1801 gttgatcgat caggtacaga ctggcgtaga gcgtggcggt ccggcccggg gcatcggcat
     1861 cgaaccgcac gtccgcccgg gtgtagtagc cgtcgccgtc actatcgccg tgcagggtga
     1921 attccagcac gaacaggtcc agatcgccgc tgctgacggc ttcccgggtc acgtcctcca
     1981 gatacagctg ggccagatcg ctgtcctgtt gcggaccgta agtgaacagc agggcattgc
     2041 tgctggcgtc atagaccctc agtgtcagtt cgtagcgggt ggctggatag ccgtcggtca
     2101 acagcacggt ggcctggtag ctgtcgctgc cggtggcacc ccggatggtg aatggatcgg
     2161 tgcggaacag gggcgtgctg gtgccgttgt tgagctggcc ggtgacgtaa atgctggcgc
     2221 tggacaggcg cgtgtcgatg tcgaaattga tgttgaactg gtggtaatag ccatcgccat
     2281 cgtcg
//
LOCUS       DASUEG010000085         4798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_724917, whole genome shotgun sequence.
ACCESSION   DASUEG010000085 DASUEG010000000
VERSION     DASUEG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724917"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2937
                     /locus_tag="VFX11_01395"
     CDS             <1..2937
                     /locus_tag="VFX11_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937302.1"
                     /translation="GRVGFMFNYYQFLAHTEFLGEVFDPEGTPSDPAANVQFVVTEVG
                     GKLKFNLFYHAHLFADVGFLQRIEALSQQILFGKTMQIGALQYVTDPAEKLLLTEIWN
                     NTAVDFDLGQCIHQRFEQQAHLHPERVAIADDQVSYSYRELNQRANQLAHHLRRQGVD
                     RNHLVGLCAERSADFLVGIIGIMKAGAAYVPMDPKYPQDRIEYMIENSEVAVLLTQEP
                     LLPKVSGNNQAKHICLDRDWADIARESTVNLNVDSSPRDRAYMLYTSGSTGLPKGALI
                     RHDGALNHIEAERRALEFKQDFSFLQTAPASSDISVWQFVGPVTCGGKVVVLDDVTHS
                     RKLFDLVKQHDISVVELVPVALQLLMEHVRSLPQEQRALPSLRWMMATGEAVSVDLVN
                     DWLALYPDTPVVNAYGPTEAADDVIQCSIHAPLPADRKSVPIGKPLANLDVFIVDEQM
                     RLVPPGVPGEICIGGIGVGEGYWKNPEKTAQAFVPNPFPGTKGGMLYRTGDLGRWLQD
                     GTVEYLDRVDNQVKVRGFRIELGEVEAAVSALPGVRENVVTVRHDMPGGTALAAYVVA
                     TDEAGTLEPTALRAQLRERLPDFMVPAAITVLDKMPLTPAGKIDRKSLPRPDSIQLGG
                     ADYVAPRSDLEAQLATIWEAFLPVERVSIRDNFFELGGHSLIGVRIMARINKELGTQL
                     QVASLLTTQTIEKLAQLIGQGGGDSNAILVPLAGGDQANSTLPALFFIHPVGGDVLIY
                     ADLANALRESFRAFGLRARGLSGAEPPFASLQEMVAGYVAAIREIQPVGPYRLAGQSL
                     GGIFALALARHLEAQGEMVADVVLLDSYSPAHLRQSHQDEAQVVGAALGRPLPVASPD
                     SGLKTEAYADWLYRTGIAAGAIPADFPRAQFEALYQVAVTNHRFASQYEVETVQANVH
                     HFTARDNITGIASGTTWQHAPLRMQCHTVPGGHETLMQGEQAATLARQLAGIFAGDST
                     DNKNKQ"
     gene            2946..4202
                     /gene="ectB"
                     /locus_tag="VFX11_01400"
     CDS             2946..4202
                     /gene="ectB"
                     /locus_tag="VFX11_01400"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX4937303.1"
                     /translation="MRTEVFQQYESEVRSYCRAFPTVFKRAKGSFIEGEDGRRYLDFF
                     AGAGALNYGHNPDFIRDKLIDYLRDDGIMHALDMYTVAKGEFIQSLQEKILLPRKLDY
                     RVQFCGPTGTNAVEAALKIARKVKGRQNIFAFQGGFHGMSLGSLACTGNQYNRKGAGT
                     TLPNVTFMPYPFGAMEKIDTIAYMDAVLSDPSSGIDKPAAVLLETVQAEGGVVVAPVE
                     WLKKLRALCDRHDILMIVDDIQVGCGRTGNFFSFERAGIVPDVITLSKSISGCGLPMA
                     ILLLKPEIDKWSPGEHNGTFRGNQLAFIGGAAALEMWTSLDIQGQVEKKSRFVESALR
                     SIIEEVDPRLSLRGLGLIWGVDFTVTGDNDLIERVLAKCFEKGLIIENAGRKGQVMKL
                     LPPLTVSESELQQGLDIIRASMKECF"
     gene            complement(4340..>4798)
                     /locus_tag="VFX11_01405"
     CDS             complement(4340..>4798)
                     /locus_tag="VFX11_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937304.1"
                     /translation="GYSVANCTGALDLEITAETTMNQIRSSIVKAMRQKEDLSPFKED
                     QKIAKWLPGAMFRQSDESLNKLHAEGRYRMTGVISYMGDVGEELFRCPGFTPKSLFGA
                     PIALENRPIFVGASTHGNVTSIFVGAPKALASQQDLQAFCTVLAERLHAL"
BASE COUNT          979 a         1459 c         1417 g          943 t
ORIGIN      
        1 ggccgtgtcg gcttcatgtt caactactac cagttcctgg cgcacaccga attcctcggc
       61 gaggtgtttg atcctgaagg cacgcccagc gatcccgccg ccaatgtgca gttcgtggtg
      121 acggaggtgg gcggcaaact gaaattcaac ctgttctacc acgcacacct gtttgccgat
      181 gtcggtttcc tgcagcggat cgaggccctg agccagcaga tcctgttcgg gaaaacaatg
      241 cagatcggcg cattgcaata cgttaccgat ccggcggaaa aattgctgct caccgaaatc
      301 tggaacaaca cggcagtgga tttcgatctc ggccagtgca ttcaccagcg tttcgaacag
      361 caggctcatc tgcaccccga gcgcgtggcg attgccgatg atcaggtgag ttacagctat
      421 cgtgaactga accagcgcgc caaccagctc gctcatcacc tgcgcaggca gggtgtggac
      481 cgcaatcatc tggtgggtct gtgtgccgag cgctcggcgg atttccttgt gggcattatc
      541 ggtatcatga aagcaggcgc cgcctacgtg ccgatggacc cgaaataccc gcaggaccgc
      601 atcgaataca tgatcgagaa ttccgaggtg gcggtactgc tcacccagga acccttgctg
      661 ccgaaagtca gcggcaacaa tcaggcaaag catatctgcc tggatcgcga ctgggccgac
      721 atcgcgcggg aatccacagt caatctgaat gtggattcct cgccccgtga ccgcgcctac
      781 atgctctaca cgtcgggttc caccggtctg ccgaaaggtg cgctgatccg tcatgacggt
      841 gcgctgaatc acattgaagc agaacgtcgc gcactggaat tcaagcagga tttcagtttc
      901 ctgcagacag cgcctgcgtc gtcggatatt tccgtgtggc agttcgtcgg cccggtcacc
      961 tgcggcggta aagttgtggt gctggacgac gtcacccatt cgcgcaaact gtttgatctg
     1021 gtgaaacagc acgacatcag cgtggtggaa ctggtgccgg tggcgctgca gctgctgatg
     1081 gagcatgtgc gttctctgcc gcaggaacaa cgtgcactgc cgtccctgcg ctggatgatg
     1141 gccactggcg aagccgtgtc ggtggatctg gtgaatgact ggctggcgct atatccggac
     1201 actcccgtgg tcaatgccta tggtcccacc gaagcggccg acgacgtgat ccagtgctcc
     1261 attcacgcgc cgctgccggc agaccgcaag agcgtcccca tcggcaagcc gctggcgaac
     1321 ctggacgtgt tcatcgtcga cgaacagatg cgactggtcc cccccggcgt gcccggtgag
     1381 atctgcattg gcggcatcgg cgttggcgaa ggctactgga aaaatcccga gaaaaccgcg
     1441 caggcgtttg tgcccaatcc attccccggc accaagggcg gcatgcttta ccgcactggc
     1501 gacctcggcc gctggctgca ggatggcacc gtagaatacc tcgaccgtgt cgacaaccag
     1561 gtgaaagtgc gcggtttccg cattgagctg ggcgaagtgg aagcggccgt gtctgcattg
     1621 ccgggcgtgc gtgaaaatgt cgtcaccgtg cgccacgaca tgcctggcgg caccgcgctc
     1681 gccgcctatg tcgtcgcgac ggatgaagcc ggcacgctgg aaccgaccgc actgcgcgcg
     1741 caattgcgcg aacgtctgcc ggatttcatg gtgcccgccg ccatcactgt gctggacaaa
     1801 atgccgctca cacctgctgg caagatcgat cgcaaatcct tgccgcgtcc tgacagcatc
     1861 cagctgggtg gcgccgatta cgtcgcgccg cgcagcgatt tggaagcaca actggccacc
     1921 atctgggaag ccttcctgcc ggtggaacgc gtcagcatcc gcgacaattt cttcgaactc
     1981 ggtggccact ccctgattgg cgtgcgcatc atggcgcgca tcaacaagga actcggtacg
     2041 caactgcagg tggcgtcgct gctcaccacc cagaccatcg aaaaactggc gcagctgatc
     2101 gggcagggtg gtggcgactc caatgccatc ctcgtgccgc tggcgggcgg cgatcaggcc
     2161 aacagcacat tgcccgccct gttcttcatc catccggtgg gtggcgacgt gctgatttac
     2221 gccgatctgg ccaacgcact gcgggagtcc ttccgggcgt tcggtctgcg cgcccggggc
     2281 ctgtccggtg ctgagccgcc ctttgcgtcg ctgcaggaaa tggtggccgg ctacgttgct
     2341 gcgattcgcg aaatccagcc cgttggaccc taccgtctgg cggggcaatc cctcggtggc
     2401 atcttcgccc tggcgctggc ccgtcatctg gaagcacagg gcgagatggt agcggatgtg
     2461 gtgctgctcg acagctacag ccccgcccat ctgcgccagt cccaccagga cgaggcccag
     2521 gtggtcggcg cggcgctggg acgtccgttg ccggtggcca gccccgattc cggcctcaaa
     2581 acagaggctt atgcagactg gctgtatcgc accggcattg cggccggtgc cattccggcg
     2641 gatttcccgc gcgcacagtt tgaagcgctt tatcaggttg ccgtcacaaa tcaccgtttt
     2701 gcttcacaat atgaagtgga aacggtacag gcaaacgtgc atcattttac ggcgcgggac
     2761 aacatcaccg gcatcgcatc cggtaccacc tggcagcacg caccgttgcg aatgcagtgc
     2821 catactgtac caggcgggca cgagaccctg atgcagggtg agcaggcggc gacactggcg
     2881 cggcaactgg ccggcatctt cgctggcgat tcaaccgata acaagaacaa gcaatgaggc
     2941 attgaatgcg taccgaagta tttcagcagt atgaatccga agtgcgttcc tactgtcgtg
     3001 cgtttcccac cgtattcaaa cgggcgaaag gctccttcat agaaggcgag gacggccgcc
     3061 gctacctgga ctttttcgcc ggcgccggcg cgctcaatta cggtcacaac ccggatttca
     3121 tccgcgacaa gctgatcgac tacctgcgtg acgacggcat catgcacgcc ctcgacatgt
     3181 acaccgtcgc caagggcgaa ttcatccagt ccctgcagga aaaaatcctg ctgccgcgca
     3241 aactggatta ccgcgtgcaa ttctgcggcc ctaccggcac caacgcggtg gaagcggcac
     3301 tgaaaatcgc gcgcaaggta aaagggcggc aaaacatttt cgccttccag ggcggcttcc
     3361 acggcatgag cctcggcagc ctcgcctgca ctggcaatca gtacaaccgc aagggggcgg
     3421 gcaccacact gccgaacgtc accttcatgc cgtacccgtt cggcgcgatg gaaaagattg
     3481 acactatcgc ctacatggat gcggtattgt ccgatcccag ctccggcatc gataaacccg
     3541 ctgccgtgct gctggaaacc gtgcaggccg aggggggcgt cgtggtggcg ccggtcgaat
     3601 ggctgaagaa actgcgcgcc ctgtgcgaca gacacgacat cctgatgatc gtcgacgaca
     3661 tccaggtagg ctgcggtcgc accggcaatt tcttctcgtt cgaacgtgcc ggcattgtgc
     3721 cggacgtgat caccctgtcc aaatccatca gcggctgcgg gctgcccatg gccatcttgc
     3781 tgctgaagcc cgagatcgac aaatggtccc cgggcgagca caacggtacg ttccgcggca
     3841 accagctggc gttcatcggc ggcgccgctg cgctggaaat gtggacgtcc ctcgatatcc
     3901 agggtcaggt ggagaaaaaa tccagattcg tggaatctgc attgcgctcg atcatcgaag
     3961 aagtcgatcc gcgcctgtcg ctgcggggtt tgggtctgat ctggggcgtg gatttcaccg
     4021 tcactggcga caacgacctg atcgagcgcg tgctggcgaa gtgcttcgaa aaaggcctga
     4081 ttattgaaaa cgccggtcgc aagggtcagg tgatgaaact gctgccaccg ctgaccgtgt
     4141 cagaaagcga gctgcagcag gggctggata tcatccgggc atccatgaag gaatgcttct
     4201 gaggcgggat gatgtggcac tgctggtgcc cgcattgatc catccgcaag tgaaatcccg
     4261 acagaaaaaa aggcccacaa aaaagggaac cgctcaggtt ccctttttta ttccgatttc
     4321 caatcaatgc gcgagtcaat caaagcgcat gcagccgttc cgccagcacc gtgcagaacg
     4381 cctgcagatc ctgctgactg gccagtgcct tgggcgcacc gacaaaaatg ctggtcacat
     4441 tgccatgggt agaagcaccg acaaatattg gccggttctc cagcgcaatg ggcgcgccga
     4501 acaatgactt gggcgtaaag cccgggcagc gaaacagctc ttcccctaca tcacccatat
     4561 agctgatgac gccggtcatc cggtagcgtc cttccgcgtg cagtttgttc agcgattcat
     4621 ccgattgcct gaacatcgca cccggcagcc atttcgcaat tttctggtct tccttgaatg
     4681 gcgaaagatc ctctttctgt cgcattgctt tcacgatcga cgagcggatc tggttcatgg
     4741 tggtttccgc cgtgatctcc agatccagcg caccggtgca gttggcaact gaatatcc
//
LOCUS       DASUEG010000086         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_393314, whole genome shotgun sequence.
ACCESSION   DASUEG010000086 DASUEG010000000
VERSION     DASUEG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393314"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFX11_01410"
     CDS             <1..183
                     /locus_tag="VFX11_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937305.1"
                     /translation="VSSTCTLRGPNGMHIQAGNHRIYWLARKIGAAPNMTVTDNSMTF
                     VCENDLLDSDGPGDGD"
     gene            complement(185..2002)
                     /locus_tag="VFX11_01415"
                     /pseudo
     CDS             complement(185..2002)
                     /locus_tag="VFX11_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
     gene            2279..2494
                     /locus_tag="VFX11_01420"
                     /pseudo
     CDS             2279..2494
                     /locus_tag="VFX11_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2628..4061
                     /locus_tag="VFX11_01425"
     CDS             2628..4061
                     /locus_tag="VFX11_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HEX4937306.1"
                     /translation="MMHAPQGNSLSLKRSTLNSASLRYRVGAVALAALLSAPLQAAVP
                     NSYAPVDDRETFDAVKSRMEKNKSAVEKKHKELLELRYDLSDKPAKGVTMSKGKAVQA
                     GVRVKLPKGTGWNDLAGMTPEQIKAKNLFPQGFLPMPHVNQAEGGMLFPKFMLDQLKK
                     QEERDLSRFDLDFVLHDRFLPEFPAPVYLTTRPDLGDVSKGKLIGLSNYYELFSGVLN
                     PKQLEGLRLLVTPFPQQQFNATIDRRTVKPSQGVACFDCHANGHTNDATHLVGDIRPQ
                     EHRHRINTPTLRGVNIQRLFGSQRALKSVEDFTEFEQRAAYFDGDPVIATKKGVNILE
                     RGSQVHFMGEFQALLDFPPAPKLNVFGRLDPSKASDAEKRGEAVFFGKGQCATCHTPP
                     YYTDNTMHNLQVERFYAPRDIDGRLASADGPIKTFPLRGIKDSPTYLHDGRLLTLDDT
                     VEFFNLVLGLQLNDQEKQDLVAFMYAL"
     gene            4216..6579
                     /locus_tag="VFX11_01430"
     CDS             4216..6579
                     /locus_tag="VFX11_01430"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX4937307.1"
                     /translation="MSRPVRMILFALLGMLALVVLAVGALVVLVDPNDYREDISTMAR
                     EGAGVELKLGGELGWSFYPVLGFRAHDVGLSLRSDAPQLVQVDAFSVGVRLLPLLSKK
                     IDVDGLDISGLKAHLIVDAKGQTNWQPPAASGDAGASAAQTSAPAGPATSPAAAEESS
                     AAIPDIRIPKITIRDSQIQYEDQQAKAAYTVDLPLLELTDVNLDEAFPLVLQARVRDN
                     AKLDLDVDLKTQVRADLAGKKFAATDLDLNTTVGGIFAAPVKAAVQGDLQFDQGADKA
                     NVKLSKLQFANVQATAQVEASAVSSAPQFKGTLKTDSFDLKALLKTLGIEPPATSDAA
                     AMTKVQADLAFSGTPEKIRIAPLQLKLDDSTLSGEAAITDVASQALRFNLKLDRLDAD
                     RYMPPESADTKSSGQVAAGGAGASGTATGASSTATSSAPAGDLIPVEPLRGLNINGTF
                     SAGEVVVKKIPVRDIGLSIKALDGDVQVSNLGAKLLQGSLSGSAGVDVRGAQPKIATN
                     IDLQNLQLGDLLQPFVKTEVLTGRSSVKLDTRTSGNDMDTLLKQALGQLNLNVADGML
                     HGVNLNQVVVDALKQKIGDFTLLMPDYETRLPKALKGDTQIRNMLANMKVENGHLITP
                     AMNADTDAGQLNATGDIDLLEQTFDYRFGVVLASLADHKYLKGTEWPVRCKGGASIPV
                     QDWCRPDMSAFGGVLQKAAGIAMRDKATEKLGEKLGIKGASEAEVKQQAEQKAKEKVN
                     QELGKQLDKLFGGKKDKKEKAPATPAPAPAETTAPAAENPATEAPAN"
BASE COUNT         1468 a         2101 c         2016 g         1198 t
ORIGIN      
        1 gtgagttcca cctgcacgct gcgcggtccc aatggcatgc atatccaggc gggcaatcac
       61 cgcatctatt ggctggcccg gaaaatcggt gccgcaccca acatgacggt gactgacaac
      121 tcaatgacct ttgtttgcga gaacgatctg ctggacagcg atggtcctgg cgatggggat
      181 tgatctagga ggaggacggg gctgccgcct tgtcctccag caaatcccct tcgaacaggg
      241 tttccagctc ggcgcgggcc tcgcgggcgg actgcatcac cgcatcctga tccccgtgca
      301 ccttgtactg ctgtttcagc acgttctcat cgtgacgctg gaagcgtcgc aggcgcgcct
      361 gcacctgttc ttccgtcaat cccagtccca gcagcaccag ccggctcatt tccatgctgg
      421 aaaaaaacgt ttcccgcacc ggcgtggcat tgctgtccat caggtggaac acatgacggc
      481 ggttgcgggc gcgcgcgatg atcttgatgt gggggaacag ctcgcgcacc tgccgcgcgg
      541 tctggatatt ggtttcggga tcgtcggtgg ccacaatgaa gaattctgcg tcggccacgc
      601 cggcggcgcg cagaatgtcc ggccgcagtg gatcgccgta ataaatcggg aaagagccca
      661 ggctgcgcac cagatcgata ctgtccaccg aggtgtccag tgcgacgaac ggcacgccct
      721 gcgcgcgcag gatacgggta atgatctgcc ccatccgtcc catgccggcc actaccacgc
      781 ggggtttgtg atcgtcgatc tgttcgtatt ccggtggcgg tttgcgcgcg agcacgggtt
      841 cgggtttcag ccagcgcccc gcggccagca gcagaagcgg tgtcagtgcc atggacaggg
      901 tgatgcacag caccagccgg ttgttgaagg cggcgtccca caggccctgg ttctgcgcca
      961 gcttgaacac cacgaaggca aattcgccgc cgcccgccag cagcagcccc agctgaaatg
     1021 cggcacgcct gttcagaccg ccgttgaagc aggcgacggc atagagcact ggcaacttca
     1081 gtgcaatcag caggcaggtc agccccagta tctgcagcgg ctcctgcacc agcagattca
     1141 ggtcggcgct catgcccaca ctcatgaaaa acagccccag caaaaggccc ttgaatggct
     1201 ggatctgcgc ttccagttcg tggcggtatt ccgaatccgc cagcagcaga cccgccagaa
     1261 atgagcccag cgccatcgac acgccggccg cttccatcag ccaggcagtg ccggccacta
     1321 ccagcagcgc ggtggcggtg gacacttccg gcagaccggt gcgggcgacg atgcggaaca
     1381 ccggccgcaa cagctagcgc ccaccgagaa ccaccagcgc cacgctgcca atcactttca
     1441 gccagggcgg caggccgtcg ctggcggcgc tggcatgtac gtcgcccagc aggggaatca
     1501 ccgcgatcag cgggatggct gcaatgtcct gaaacagcag aatggaaaat gccagccggc
     1561 catgagggct gttgagctgc ttgctctccg ccagaatctg caggccgaat gcagtggacg
     1621 acagcgccag ccccaggccc agcaccaggg ccgcattcca gtgctggtca aaaccccaga
     1681 acgccaccgc gcccagcacg gcgcaggtca gcagcacctg cagcagtccg ccgccgaaca
     1741 ccgagcgccg catcacccac agccggtgcg gtgacagttc cagcccgatg atgaacagca
     1801 acagcaccac cccgagttcc gagatatggc ccacgctggc gggatcgccc accagcccca
     1861 gcagcgacgg cccgatcagg cagcccgcca gcaggtagcc catcacggcg cccagtcgca
     1921 ggcgtttggc cagcggcacc gcaaacacgg cggccagcag gaaaatgacg gcggaaccga
     1981 ggaaagaatc ggtgtgttcc aacgggattg ctccaggaag atgggggagg gcccagatta
     2041 ggggcggttg gcacggctgt aaacggtcaa ttggtacgca aacgcacaga ccgaagagtt
     2101 ccattgattg aaactatctt gtgccacagt tttatcaatt ggcgggatca tgatcatgga
     2161 gctagcctca ttttcacatt gctgcctgga ccgacatcca ggcttctttc tattcaggta
     2221 tttcccgtcc agtagtcacc gcagctgcct gatcttccaa caaccgatgg agcgcatcat
     2281 gaccctcaag gaactggcct atatcgtggc cgtggcagag gaactgcatt ttcaccgcgc
     2341 cgccgagcgc tgccatgtca ctcagtccac cctcagcaac cagttgaaaa agtgcgagga
     2401 ctatctcggg gtcaccattt ttcgccgcaa ccggcatcag gtggagctga ccccggaagg
     2461 cgaaaaaatc gtcgcgctgg cccagcaggc gctggcggcc accgatgcca ttcgtcgcta
     2521 ttcccgccag caggcgttgc tggacacgct gtaattcaca caatcaatca cgccgatttt
     2581 tcagctggtt agaattccct gaacctggac aaaaaaagag ggaattgatg atgcacgcac
     2641 cgcagggaaa ttcactgtcg ctaaaacgat caactttaaa ttccgcgtca ctccgttacc
     2701 gggtgggtgc tgttgccctg gccgcgctgc tgtctgcgcc cctgcaggca gccgttccca
     2761 attcctacgc acccgtggac gaccgcgaaa ccttcgacgc cgtgaaatcg cgcatggaaa
     2821 aaaacaaaag cgcggtggag aaaaagcaca aggagttgct ggagctacgc tatgacctga
     2881 gtgacaagcc ggcgaagggc gtcaccatgt cgaaaggcaa ggcggtgcag gcgggcgtgc
     2941 gggtgaaact gcccaagggc actggctgga acgatctggc gggcatgacg ccggaacaga
     3001 tcaaggcgaa aaacctgttt ccgcagggct ttctgccgat gccgcatgtg aatcaggccg
     3061 aaggcggcat gctgtttccc aaattcatgc tcgaccagtt gaaaaagcag gaagagcgcg
     3121 atctgtcccg cttcgatctg gattttgttt tgcacgaccg ctttctgccg gagtttcccg
     3181 cgccggtgta tctcaccacg cggccggatc tgggcgatgt gagcaaaggc aagctgattg
     3241 gcctgagcaa ttactacgaa ctgttttccg gcgtgctgaa tccgaaacag ctggaagggc
     3301 tgcggctgct ggtgacaccg tttccgcagc agcagttcaa tgccaccatc gaccgtcgca
     3361 cggtgaaacc cagccagggc gtggcctgtt ttgattgtca tgccaacgga cacaccaatg
     3421 acgccaccca tctggtgggc gacatccgcc cgcaggaaca ccgtcaccgc atcaacacgc
     3481 ccaccctgcg cggcgtgaat atccagcgcc tgttcggttc gcagcgggca ttgaaatcgg
     3541 tggaggattt caccgagttc gaacagcgcg ccgcctattt cgatggtgat ccggtgattg
     3601 ccaccaagaa aggcgtaaat attctggagc gcggcagcca ggtgcatttc atgggcgagt
     3661 tccaggcgtt gctggatttt ccgccggcac cgaaactgaa tgtgttcggc cggctggacc
     3721 cgagcaaggc cagcgatgcg gaaaaacgcg gtgaagccgt gttcttcggc aagggccagt
     3781 gcgccacctg ccacaccccg ccgtactaca ccgacaacac gatgcacaac ctgcaggtgg
     3841 agcgcttcta tgcaccgcgc gacatcgatg gccgcctggc cagtgccgat ggccccatca
     3901 agactttccc gttgcggggt atcaaggatt cgcccaccta tctgcacgat ggccgtctgc
     3961 tgacgctcga cgacacggtg gaattcttca acctggtgct gggcctgcag ctcaacgatc
     4021 aggagaaaca ggatctggtg gcgttcatgt acgccttgta aattccttcc gaatgcaagg
     4081 gtgaatccgc acaaccgcga cgcaatgtga tctggcgcgc ggttgtgttc ctgcctgcgt
     4141 ccgcccccta cgcatcgctc cgtcactggt gtacccttga cggcaattca agtcggtcaa
     4201 ggaaagggga atcttatgtc gcgtcctgtt cgcatgattc tgttcgcgct gctcggcatg
     4261 ctggcactgg tggtgctggc cgtgggcgcg ctggtggtgc tggtggatcc caacgattac
     4321 cgcgaggaca tcagcacaat ggcgcgggaa ggcgccggtg tggaattgaa actgggcggt
     4381 gaactgggct ggagtttcta tccggtgctg ggtttccgcg cccatgatgt gggcctgtcg
     4441 ctgcgcagcg acgcgccgca gctggtgcag gtggatgcgt tttccgtggg cgtgcgcctg
     4501 ctgccgctgc tgtcgaagaa aatcgatgtg gatggactgg acatcagcgg gctgaaagcg
     4561 catctgatcg tcgatgccaa aggccagacc aactggcaac cgcccgcagc gtccggtgat
     4621 gccggcgcca gtgctgcaca gaccagtgcg cctgccggtc cggcgacatc acctgccgcc
     4681 gccgaagaat cctcagccgc catccccgac atccgcatcc ccaaaatcac cattcgtgat
     4741 tcgcaaatac aatacgaaga ccagcaggcc aaagccgcct acacggtgga cctgccgctg
     4801 ctggaactca ccgacgtgaa tctggacgaa gcgtttccgc tggtgctgca ggcgcgggtg
     4861 cgcgacaacg ccaaactgga tctggacgtg gacctgaaaa cccaggtgcg cgccgatctc
     4921 gccggcaaga aattcgctgc caccgatctg gacctgaata ccacagtggg cggtattttc
     4981 gctgcgccgg tgaaagcagc cgtgcagggc gatctgcagt ttgaccaggg cgccgacaag
     5041 gcgaacgtga aactgtcgaa gctgcaattc gccaatgtgc aggccactgc ccaggtcgag
     5101 gccagcgccg tcagcagcgc tccgcaattc aagggcacac tgaaaaccga cagcttcgat
     5161 ctgaaagcct tgctgaaaac gctaggcatt gaaccgcccg ccaccagtga cgctgcggcc
     5221 atgaccaagg tgcaggcgga tcttgccttc agtggcacgc cggaaaaaat ccgcatcgcg
     5281 cccctgcagc tgaaactgga tgacagcacc ctgagcggcg aggccgcgat caccgacgtg
     5341 gcctcgcagg cactgcgttt caacctgaaa ctcgacaggc tggacgcgga ccgttacatg
     5401 ccgccggaat ccgccgacac gaaatcatct ggccaggttg ctgctggcgg cgctggtgcg
     5461 tctggcactg cgacaggcgc gtcatccacc gccacgtcat ccgcgcctgc cggcgatctg
     5521 attccggtgg aaccgttgcg tggactcaat atcaacggca ccttctctgc cggcgaagtg
     5581 gtggtgaaaa aaattccggt gcgcgacatc ggtctcagca tcaaggcact ggatggcgat
     5641 gtgcaggtga gcaatctggg cgcaaaactg ctgcaaggct ccctgagcgg ttctgccggc
     5701 gtggatgtgc gcggtgcgca accgaaaatt gccaccaata tcgatctgca gaatctgcaa
     5761 ctgggtgatc tgttgcagcc tttcgtgaag acagaagtgc tcaccggccg ctcctcggtg
     5821 aagctcgata cccgcaccag cggcaacgac atggacacgc tgctgaagca ggccctgggc
     5881 cagctcaacc tgaatgtggc ggacggcatg ctgcacggcg tcaacctgaa ccaggtggtg
     5941 gtggatgcgc tgaaacagaa aatcggcgac ttcacactgc tgatgcccga ctatgaaacc
     6001 cgcttgccca aggccctgaa aggcgacacg caaattcgca atatgctggc caacatgaaa
     6061 gtggaaaacg gtcacctgat cacgccagcg atgaacgccg acaccgacgc cggccagctc
     6121 aacgccacgg gcgacatcga tctgctggag cagacattcg attaccggtt tggcgtggtg
     6181 ctggcgtcgc tggcagacca caaatacctg aaaggcaccg aatggccggt gcgttgcaag
     6241 ggcggcgcat cgattccggt gcaggactgg tgtcgccccg acatgagtgc cttcggcggt
     6301 gtgctgcaga aagccgccgg cattgccatg cgcgacaagg ccacggaaaa actcggcgag
     6361 aaactgggca tcaagggtgc cagcgaagcg gaagtgaagc agcaggccga gcagaaggcg
     6421 aaggaaaagg tgaaccagga actgggcaaa cagctcgaca aactgtttgg tggcaagaag
     6481 gacaagaagg aaaaggcacc ggccacgccg gcacctgctc cggctgaaac gactgcgcct
     6541 gccgctgaaa accctgcgac tgaagctcct gccaactgaa caatgcagcg ccggctggaa
     6601 cgttcactct gccggcgctg taatccccat ctcaatcccc gccatcccgc ctaactcctt
     6661 tcggcacgcc tccatggcgc cgtgcccaaa ctcaaccata ctttcaatat tgccaatccc
     6721 tattccctga caggcccaat cccatggtgt cgacccagcc tctgccgagc aatgcacccg
     6781 tga
//
LOCUS       DASUEG010000087         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1124938, whole genome shotgun sequence.
ACCESSION   DASUEG010000087 DASUEG010000000
VERSION     DASUEG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124938"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VFX11_01435"
     CDS             <1..122
                     /locus_tag="VFX11_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX4937308.1"
                     /translation="RSMATGYAGLDNDLFYMDKSMMIFGDAKKVVEDMIKGLH"
     gene            419..1321
                     /locus_tag="VFX11_01440"
     CDS             419..1321
                     /locus_tag="VFX11_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937309.1"
                     /translation="MKRKIVPSRLSLAVLGMLAFFAITLEATAATTCPAGAICRYEEA
                     PGSYSGNGPYTVRSYTLSRLQTPGGATVYYPSNARPPFSGVVFTPPYTGIQSMFAAWG
                     PFFASHGIVMVTMDTSTTLDTVDQRASQQKQVLDALKRENTRSASALYGKLNTARLGA
                     VGWSMGGGATWINSAEYAGLKSAMSLAGHNLTAVDIDSRGGNTRIPTLILNGALDLTY
                     LGGLGQSDGVYNNIRSGVPKVFYEVSSAGHFDWGSPTAANRAVAGIALAFHKTFLDGD
                     TRWVSYIRRPSSDVSKWATASLPQ"
     gene            1400..2029
                     /locus_tag="VFX11_01445"
     CDS             1400..2029
                     /locus_tag="VFX11_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937310.1"
                     /translation="MSDRKRWRISALVAAAVASAMLFAALSRDIGKPAATASTPLTDS
                     VAPLPDWSWQQDAGVRPVVAEESNAAAVDEQQIDMDAVSSDGTDDQSDEISLTKRELE
                     YLKEALPGNLVVPAEKTPAEVEALFAEFDEYQALARRMEEGTASAEERSRYYDIRMAK
                     FQEEIALINLCNDVLVNQASTPLCANIAATSAERLADIEKSMQALEQDW"
     gene            complement(2246..3556)
                     /locus_tag="VFX11_01450"
     CDS             complement(2246..3556)
                     /locus_tag="VFX11_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4937311.1"
                     /translation="MISVEPSALVSLFDQPAIFIDRQYRILASNGAYQQRYGVPKPDC
                     HCFQASHGYSSPCHANGEPCPLLQSQQTGTHARALHIHQDRDHRHFCQVEMHPVQDSS
                     GATVGFIEIMNAVESASVNAGNKGLIGESPAFQKMMEAVYRVAPTDVSVLLEGESGTG
                     KELVARAIHDASNRKLGKFVVLECSGINENLFESELFGYRKGAFTGARSDKTGLAASA
                     SGGTLFLDEVGDIPINLQVKLLRLLETGAFRPVGATEMERTDFRLICASHKNVKAMVE
                     AGTFREDLYYRLATFPIHLPPLRERREDLALLCEAMLGRPPVPAGTHVSNAVIKQLAR
                     YRFPGNIRELRNLIIRASLLAEEHVIQTKHLPPEIAQQTAPRDKTHFAENVLPLEQIE
                     LDYLRHIQEVFEGSSAELALKLGISERTLYRKLARLKSNTVADT"
     gene            3749..3922
                     /locus_tag="VFX11_01455"
     CDS             3749..3922
                     /locus_tag="VFX11_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937312.1"
                     /translation="MTRPALSLRKHLFWLLLIKLAAIIAIKLTFFPSLKAEHPPADLF
                     EPASFIAVPKESS"
     gene            3919..5511
                     /locus_tag="VFX11_01460"
     CDS             3919..5511
                     /locus_tag="VFX11_01460"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006881377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX4937313.1"
                     /translation="MIDADLVEVSRLQFALTALFHFIFVPLTLGMSFLLAIMESAYVL
                     TQKTIYKDMTRFWGKLFGINFALGITTGITLEFQFGTNWAYYSHYVGDIFGVPLAIEG
                     LMAFFLESTLIGLFFFGWERLSQRQHLAVTWLVALGSNLSALWILIANGWMQNPVGAE
                     FNYQTMRMEMTSFAALLFNPVAQVKFVHTVAAGYVTGAMFVLGVSSYYLLRQRDVPFA
                     RRSFAIAASFGTASILSVIVLGDESGYEMGDVQKVKLAAIEAEWHTHEPPAAFTLVGW
                     PDDEQEKTHFAVRIPYVLGIIATRSFDKPVLGISDLKKINEGRIREGMVAYGLLQKLR
                     NGDTSDETRVAFDQVKDHLGYGLLLKKTSPTVVDATEADIQAAVDRSIPKVWPLFWSF
                     RVMVGAGLFMLVVFVLAFWQSTRHRVAEKKWLLKLAMWSIPLPWIAAECGWIVAEYGR
                     QPWTIGGILPTDLSVSNLAVEDVWSSLIAIVLFYTALFVVEMYLMLHFIRKGPGSLGT
                     GKYHGEQTAPLDPNSSATGAHA"
BASE COUNT         1083 a         1522 c         1756 g         1250 t
ORIGIN      
        1 agcgttcgat ggcaaccggt tacgccggtc tggacaacga tctgttctac atggacaagt
       61 cgatgatgat tttcggcgac gcgaagaaag tcgtggaaga catgatcaag ggtctgcact
      121 gatcagtgcc ggattccggt ccacccaaaa cgcgccttcg ggcgcgtttt tttatgcctg
      181 acagatggcc gtagcgaccg aaaacactgg caatgtggcc aatccggcaa cgtcgaccgg
      241 gtgtttgccg gaccccggga ttattcttcc cccgctcgtt cgcgagcgct gacaccaacg
      301 aaattcctct attcccgtgg tgatttcgca gttcggcagt aacttgctca gggaggcagt
      361 tagcagggcg taatggtgag aaacacatca tccattctcc ggaaaaaagg aaattttcat
      421 gaaaagaaaa atagtcccct cccgtttgtc attggctgtt ttgggaatgc tggcgttctt
      481 tgcgattacg ctggaagcaa ctgctgcaac cacctgcccc gcaggtgcca tctgccgtta
      541 tgaagaggcg ccgggttcct acagcgggaa cggtccctat acggtgagaa gctatacgct
      601 gtccagattg cagacgccgg gtggcgcaac tgtttattac ccgtccaatg ccaggccgcc
      661 gttttcaggc gtcgttttca ctcctcccta taccggcatc cagtccatgt ttgcagcctg
      721 gggtccgttc ttcgcttccc atggcattgt gatggtgacc atggatacca gcaccacgct
      781 ggacacagtg gaccagcgcg ccagtcagca gaagcaggtg ctggatgcgc tgaagcggga
      841 aaatacccgc tccgccagtg cgctgtatgg caagctgaat acagcacgac tgggagcagt
      901 gggctggtcg atggggggtg gtgccacctg gatcaactcc gctgaatatg ccggcctgaa
      961 gagcgccatg tcactggccg ggcacaatct gacggcggtg gatatcgatt cgcggggtgg
     1021 caacacgcgt attcccaccc tgattctgaa tggcgcactg gatctcacct acctgggtgg
     1081 attggggcag tccgatggcg tctacaataa tattcgcagc ggagttccca aggtgtttta
     1141 tgaagtgtcc agcgcgggcc atttcgactg gggctcaccg accgcggcca acagggcggt
     1201 tgctggcatt gcgctggcat tccacaagac ctttctggat ggcgacacgc gctgggtgag
     1261 ttacatcaga cgccccagct cggacgtctc gaagtgggca acagcaagcc tgccgcagta
     1321 atcgcgcgga cggatgcggt gtgtgccggc ggtagcaccg gcacacacct tttcctggac
     1381 acaggaggac ggattcacga tgagtgacag aaagcgctgg cggatttcag cattggtggc
     1441 ggcagccgtt gccagtgcaa tgctgtttgc tgcgttgtcg cgcgatattg gcaagccggc
     1501 agccacagca tcgaccccgc tgacagattc ggtggcgccg ctgcctgatt ggtcctggca
     1561 gcaggatgca ggtgtgcggc cggttgttgc agaggaaagt aacgccgctg ctgttgatga
     1621 acaacagatt gatatggatg ccgtgtcgtc agacggcact gacgatcaga gcgacgaaat
     1681 cagtctgacg aagcgggagc ttgagtatct gaaagaagca ttgcccggca atctggtggt
     1741 gcccgcggaa aaaacgcccg ccgaagtgga ggccctgttt gctgagtttg atgaatacca
     1801 ggcgctggcc aggcgcatgg aagagggcac tgcctcggcc gaagagcggt cacgctatta
     1861 cgatatccgg atggcgaagt tccaggaaga aattgcgttg atcaacctgt gtaacgatgt
     1921 gctggtcaat caggcgtcca cgccactgtg cgccaacatc gcggcaacca gcgcagagcg
     1981 gctggctgac atagaaaaat ccatgcaggc gctcgagcag gactggtagt tttcccgcgc
     2041 tgaaactgac agcacagaaa ttctgctatg cattgaagat gtcacaggcc ggattgtttc
     2101 atgtgctgat gggtgcagct acctgtccgg ctgatctggc gctgctgatt gttgcgttca
     2161 atccgacgct ggcagaaagg tcgctgtcat cgccacccgg ccgtgtttta ttgcggggct
     2221 tccaaccata aagaaccaca gaacgtcagg tgtccgccac tgtattggat ttcaatcgtg
     2281 ccagcttgcg atacagcgta cgttcgctga tccccagttt cagtgccagc tcggcgctcg
     2341 acccttcaaa cacttcctgg atgtgacgca ggtaatccag ttcgatctgc tccagcggca
     2401 gcacgttttc cgcaaagtgg gttttgtccc gcggcgctgt ttgttgtgca atttccggtg
     2461 gcagatgctt ggtctggatc acatgttcct cggccagcag cgaggcgcgg atgatgagat
     2521 tgcgcagctc gcggatattg ccgggaaaac ggtagcgcgc cagctgcttg atcacggcgt
     2581 tgcttacgtg agtgccggcc ggtaccggtg gtcgccccag catggcttcg cacagtaacg
     2641 cgagatcctc gcgccgttcc cgcagcggcg gcagatgaat ggggaaggtg gccaggcggt
     2701 agtacagatc ctcgcggaag gtaccggctt ccaccatggc tttcacgttt ttgtggctgg
     2761 cgcagatcag gcggaaatcc gtgcgttcca tttccgtggc gccgaccggg cggaaggcac
     2821 cggtctccag cagccgcagc agtttcacct gcaggttgat gggaatgtcg cccacttcat
     2881 ccagaaacag ggtgccaccg ctggcgctgg cggcaaggcc ggtcttgtcg ctgcgggcgc
     2941 cggtgaaggc gcctttgcgg taaccgaaca gttcgctctc gaacaggttt tcgttgatgc
     3001 cgctgcattc cagcaccacg aacttgccca gcttgcggtt gctggcgtcg tggatggcgc
     3061 gggccaccag ttccttgccg gtgccggatt cgccttccag caatacgctt acatccgtgg
     3121 gcgcgacgcg atacaccgct tccatcattt tctggaacgc cggagattcg ccgatcaggc
     3181 ccttgttgcc ggcattgacg ctggcggatt ccaccgcatt catgatttcg ataaagccga
     3241 cagtggcgcc gctgctgtcc tgcaccgggt gcatttccac ctggcagaaa tggcggtggt
     3301 cgcggtcctg gtggatatgc agggcgcggg cgtgggtgcc ggtctgctgg gattgcagca
     3361 gcgggcaggg ctcgccgttg gcgtggcagg gtgagctgta gccgtgtgac gcctggaagc
     3421 agtggcagtc gggtttgggc acgccgtagc gctgctggta ggcgccgttg ctggccagga
     3481 tgcggtattg ccggtcgatg aaaatggcgg gctgatcgaa cagggaaacg agggcggaag
     3541 gctcgacaga gatcatacgg gctcctgtca gatttggcag taggtgcctg tcaaatatga
     3601 ccctgtgctg tacggtgcgc aaccgctgat tttttcgatc gacaaataat gtcgattttt
     3661 caataggata attatttctg gcggcgcgaa tttgctggca cgctcggtgc taattccttg
     3721 cttacaatcc gtcggcaagg tgaatttcat gaccaggccc gccctgtccc tgcgcaagca
     3781 ccttttctgg ctgctgctga tcaagctggc ggcgatcatt gcgatcaagc tgactttttt
     3841 cccgtccctg aaggcggagc atccgcccgc cgacctgttc gagcctgctt cattcattgc
     3901 cgttcccaag gagtcctcat gatcgacgct gatctggtgg aagtgtcgcg cctgcaattt
     3961 gcgctcacgg ccctgtttca tttcatcttt gtgccgctca cgctgggcat gtcgtttttg
     4021 ctcgcgatca tggagtcggc gtatgtgctc acgcagaaaa ccatctacaa ggacatgacg
     4081 cggttctggg gcaagctgtt cggcatcaat ttcgcgctgg gcatcaccac cggcatcacg
     4141 ctggaattcc agttcggcac caactgggcc tactactcgc actacgtggg cgacatcttt
     4201 ggcgtaccgc tggcgattga agggctgatg gcgttttttc tggaaagcac gctgatcgga
     4261 ttgttctttt tcggctggga acgcctgagc cagcgccagc acctggcggt gacctggctg
     4321 gtggcgctgg gttcgaatct gtcggcgctg tggattctga ttgccaatgg ctggatgcag
     4381 aatccggtgg gcgcggaatt caattatcaa accatgcgca tggaaatgac ctcgtttgca
     4441 gcgctgctgt tcaacccggt ggcgcaggtg aaattcgtgc acacagtggc ggccggttat
     4501 gtcaccggtg cgatgtttgt gctgggcgtg tccagttact acctgctgcg ccagcgtgac
     4561 gtgccgtttg cgcgccgctc gtttgcgatt gcggcgagtt ttggtacggc gtcgattctg
     4621 tcggtgatcg tgctgggtga tgaatccggc tatgaaatgg gggatgtgca gaaggttaag
     4681 ctcgcggcca tagaagcgga gtggcacacc cacgaaccgc ccgctgcatt taccctggtg
     4741 ggctggcctg atgatgagca ggagaaaacc cacttcgcgg tgaggattcc gtatgtgctg
     4801 ggcatcatcg ccacgcgctc gttcgacaag ccggtgctgg gcatttccga tctgaaaaaa
     4861 atcaacgagg ggcggattcg cgaaggcatg gtggcttacg gcctgttgca gaaattgcgc
     4921 aacggcgaca ccagcgacga aacccgcgtg gcatttgatc aggtcaaaga ccatctcggc
     4981 tacggtctgc tgctgaaaaa aaccagccca actgtggtgg acgcgacgga ggcggacatc
     5041 caggcggcgg tcgaccgcag cattcccaaa gtctggccgc tgttctggag ttttcgcgtg
     5101 atggtgggcg cgggcctctt catgctggtg gtattcgtgc tggcgttctg gcagtccacc
     5161 cgccaccgcg tcgcggagaa aaaatggctg ctgaagctgg caatgtggtc cattccgcta
     5221 ccctggatcg cggcggagtg cggctggatc gtggcagagt acggtcgcca gccctggacc
     5281 atcggcggca ttctgcccac ggatctgtcg gtgtcgaatc tggcggtgga ggacgtgtgg
     5341 agcagtctga tcgcgatcgt gctgttttac acggcgctgt tcgtcgtcga gatgtatctg
     5401 atgctgcatt tcatccgcaa ggggccgggc agtctgggta caggaaaata tcacggggag
     5461 cagacagcgc cgcttgatcc gaattcttcc gccacaggag cacacgcatg attaccgatt
     5521 acgaaacatt gaaactggtc tggtgggtac tggtgggggt gctgctgatc ggcttcgccg
     5581 tcaccgacgg cttcgacatg ggagtgggca c
//
LOCUS       DASUEG010000088         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_592185, whole genome shotgun sequence.
ACCESSION   DASUEG010000088 DASUEG010000000
VERSION     DASUEG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592185"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFX11_01465"
     CDS             <1..203
                     /locus_tag="VFX11_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEX4937314.1"
                     /translation="LVEEFVTREGTDYGQQEVALERKVAQVVNGIRRKDYVIVFDSEA
                     ESTHILTRQQWREAARQSGLDV"
     gene            325..765
                     /locus_tag="VFX11_01470"
     CDS             325..765
                     /locus_tag="VFX11_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4937315.1"
                     /translation="MKSVRDILRSKAISHTFSVQPGTKVLDAIKLMAEKGIGALVVLE
                     DGKLVGIVSERDYARKVVLMARSSHTAEVREIMTADVITVEPGQSIEHCMELMTERRL
                     RHLPVLEDGQLLGMISIGDIVKHIISDQQFLIRQLEGYIRGEAV"
     gene            complement(701..>2548)
                     /gene="mnmC"
                     /locus_tag="VFX11_01475"
     CDS             complement(701..>2548)
                     /gene="mnmC"
                     /locus_tag="VFX11_01475"
                     /inference="COORDINATES: protein motif:HMM:NF002481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEX4937316.1"
                     /translation="ETGFGTGLNVLCAWELWRQARKPGQHLHVVSVEKFPLGRDDLER
                     ACTLRPDFADMAAQLLAQYPPLVPGSHRLHFHGEQLTLTLIFGDAIDSFAALDGQVDA
                     WFLDGFAPARNPDMWRPELFAQLARLSHAGTTFATFTAAGDVRRGLQDAGFSVEKCAG
                     FGRKRDMLKGLFTATDKPATFPPAQAPWFCFDYRRQTPGHAAIIGAGLAGCTAAHALA
                     LRGWQVTVLEAENSIATQASGNPTGITYTRLSVHDSPQNRYYQFAYLHACRFVRDFFA
                     ARKMEPGTDWNLNGVLQLAWDENEHTEQQKLLASGLWPSEVVEALTPEQVSVLTCTPC
                     PHPALLMKMGGWLNPATLCQALLQHRNIQLRTNFRAEQFTRTDSRWHINNDSTPFDAV
                     VLANTFESSQYAFTQHLPLRWVRGQISYVPATPASQQLQHAINYDGYLNPARDGFHCI
                     GATFNPKDKDPQQREQDHHKNLQQLRDTWPDMAAALQLDASKKMEGRVGFRCQTPDYL
                     PVAGPLPDPQTFAQNYEDIGKGFLKREFGRAPLLPGLYITTGHGSKGITSSLLAADIL
                     AGYITGEPQPVDRAVLFAVHPARFLLRGIRRPRREYTLPADGSGTADRK"
BASE COUNT          508 a          733 c          814 g          493 t
ORIGIN      
        1 cgctggtgga ggaattcgtc acccgcgagg gcaccgatta cggccagcag gaagtggcgc
       61 tggagcgcaa ggtggcccag gtggtgaacg gcatccggcg caaggattac gtgatcgtct
      121 tcgacagcga ggccgaatcc acgcatattc tgacccggca gcagtggcgc gaggcagcac
      181 ggcagtccgg actggatgtc tagcgtattg aaaaaccacg ggaaaacccg gggaatttag
      241 tgcaattgtc aaaccccttg gaacagtagt aagctcggaa aacactccaa tttataaaaa
      301 tcaataaaaa cgaggacggt gcggatgaag agcgttaggg atattttgag gtccaaggcc
      361 atttctcaca ccttcagtgt gcagccgggc accaaggtgc tggacgccat caagctgatg
      421 gcggaaaaag gaattggcgc gctggtggtg ctggaagacg gcaagctggt gggcatcgtc
      481 agcgagcggg attacgcacg caaggtggtg ttgatggcgc ggtcctccca cacggccgag
      541 gtgcgggaaa tcatgaccgc cgacgtgatt accgtggagc ccggccagag catcgagcac
      601 tgcatggaac tgatgactga acgccgcttg cgtcacctgc cggtgctgga agacggtcag
      661 ttgctgggca tgatctccat cggcgatatc gtgaagcaca tcatttccga tcagcagttc
      721 ctgatccgtc agctggaagg gtatattcgc ggcgaggccg tctgattccc cgcagcagaa
      781 accgcgccgg gtgcactgcg aacaagacag cgcggtccac cggttgcggc tcacccgtga
      841 tgtaacccgc cagaatgtcc gccgccagca atgacgatgt aattcccttt gaaccgtgac
      901 cggtggtgat gtacagaccc ggcagcaacg gcgcacgtcc gaattcccgt ttcagaaatc
      961 cctttccaat atcctcataa ttctgcgcga atgtttgcgg atctggcagc ggcccggcca
     1021 cgggcagata gtccggcgtc tggcagcgga aacccacccg gccttccatt tttttgctgg
     1081 catccagctg cagcgcggcg gccatgtccg gccaggtgtc gcgcagttgc tgcaggtttt
     1141 tgtggtgatc ctgctcccgt tgctgcgggt ctttgtcttt cggattgaag gtggcgccga
     1201 tgcagtggaa gccatcccgt gccggattca gatagccgtc gtagttgatg gcatgctgca
     1261 gctgttgcga tgcgggtgtg gccggcacat aactgatctg gccgcgcacc cagcgcaacg
     1321 gcagatgttg ggtgaaggca tactggctgg attcaaaagt gttggccagt accacggcat
     1381 cgaagggcgt gctgtcgttg ttgatgtgcc agcgggaatc ggtgcgcgtg aactgttctg
     1441 cgcgaaagtt ggtgcgcagc tggatattgc gatgctgcaa caacgcttgg cacagtgtcg
     1501 ccggattcag ccagccgccc attttcatca gcagggccgg gtgagggcag ggcgtgcacg
     1561 tcagcacgga tacctgctcc ggtgtcagcg cttccaccac ttcgctgggc cacaggcccg
     1621 aggccagcaa tttttgctgc tcggtatgct cgttttcatc ccacgccagt tgcagcacgc
     1681 cgttgagatt ccagtcggtg cccggttcca ttttgcgcgc ggcaaaaaaa tcccgcacga
     1741 aacggcaggc gtgcagatag gcgaactggt agtaacggtt ttgcggcgag tcgtgcacgc
     1801 tcagacgggt gtaggtgatg ccggtgggat tgcccgaggc ctgcgtcgca atgctgtttt
     1861 ccgcttccag cacggtgacc tgccaacccc gcaatgccag tgcatgcgca gcggtgcaac
     1921 ctgccagacc ggcgccgatg atggcggcat ggcccggggt ctggcgccgg tagtcaaaac
     1981 aaaaccaggg cgcttgtgcg ggcggaaagg tggcaggctt gtcagtggca gtgaacaggc
     2041 ccttcagcat gtcgcgcttg cggccgaagc ccgcgcattt ttccacgctg aaaccggcgt
     2101 cctgcagtcc gcgccgcaca tcgccggcgg cggtaaaggt ggcaaaggtg gtgccggcgt
     2161 gactgagccg cgccagttgc gcgaacagtt ccggccgcca catgtcggga ttgcgggcgg
     2221 gagcgaaccc atccagaaac caggcgtcca cctggccgtc cagtgccgcg aagctgtcga
     2281 tggcatcgcc gaagatcaac gtcagcgtca gttgctcacc gtgaaaatgc aggcgatgac
     2341 tgccgggcac cagcggcggg tattgcgcca gcagttgcgc ggccatatcc gcaaaatccg
     2401 gtcgcagcgt gcaggcccgc tccagatcgt cccttcccag cggaaatttt tccacgctca
     2461 ccacgtgcag atgctgtccc ggtttgcgcg cctgccgcca cagttcccag gcgcacagaa
     2521 cattcaggcc ggtgccgaag ccggtttc
//
LOCUS       DASUEG010000089         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_923535, whole genome shotgun sequence.
ACCESSION   DASUEG010000089 DASUEG010000000
VERSION     DASUEG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923535"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1470
                     /locus_tag="VFX11_01480"
     CDS             10..1470
                     /locus_tag="VFX11_01480"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4937317.1"
                     /translation="METDVLIIGTGFGGIGLAIRLLQAGRSRFVMLEKAADVGGVWRE
                     NTYPGAACDVPSHLYSFSFERQFDWSRTFAPQSDILRYLRHCVDHYGLRPRIHTGKEV
                     SSARYDRTSARWDVECHDGSHYRCRVLVAATGQLCRPLMPALPGLDQFRGVLFHSAQW
                     RHDVDLAGKSVAVIGTGASAIQFVPTLARQAAKLTLFQRSPPWILPKPERLYTGAWGR
                     LFRALPWLVTLHRLWIYIALEYRQPMLRGFRPWMALVERMFRQHLEEQVSDPDLRARL
                     TPDYPLGCKRILISDDYYPALQQPNVTLVNEPVARLEADAIVSSSGQRFPVDCVILGT
                     GFDTTHFLAPIQVTGLHGQTLADTWKDGAEAYLGLTVHGFPNLFLLYGPNTNLGHNSI
                     VYMLESQFNYVLSALDALGQYPQPAAALNVRADVQQAFNRGLQQQLAKSVWSGGCRSW
                     YVDAQGHNANNWPGFTFRYRALTRRLNPGDYEWVGG"
     gene            complement(1476..1943)
                     /gene="smpB"
                     /locus_tag="VFX11_01485"
     CDS             complement(1476..1943)
                     /gene="smpB"
                     /locus_tag="VFX11_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX4937318.1"
                     /translation="MSNKKDGGGTIALNKKAKHEYFIEERFEAGLALQGWEVKSLRAG
                     KVNLLDSYVLMKDGEGYLFGAHISPLPTVSTHFVPDPLRSRKLLLHKRELGRIFGAIA
                     KQGMTCVPLALYWKNGNVKCEIALAKGKQLHDKRATERDRDWNREKGRLLKNG"
     gene            2099..2407
                     /locus_tag="VFX11_01490"
     CDS             2099..2407
                     /locus_tag="VFX11_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX4937319.1"
                     /translation="MGDSIRVEVAYALPDRQLVVPLQVKKGTTLFEAAVQSGIATKFD
                     GLDLEKAPMGVFGKAERKPKERVLEDGERVEIYRPLIADPKEVRKKRAAAKMDDAEDD
                     "
     gene            complement(2417..>2629)
                     /locus_tag="VFX11_01495"
     CDS             complement(2417..>2629)
                     /locus_tag="VFX11_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937320.1"
                     /translation="GKGIFIENHLVLYFEGERLARIDNTEYKDPEQLRNDLMKQLGIE
                     VKQNTTEMGTPAPVEEPRDAGPAAPI"
BASE COUNT          469 a          833 c          816 g          511 t
ORIGIN      
        1 caaggcagaa tggaaaccga cgtactgatc attggcaccg gcttcggcgg cattggcctg
       61 gcgatccggt tgctgcaggc aggtcgcagc aggtttgtga tgctggaaaa agccgccgac
      121 gtgggcggtg tgtggcggga aaatacctat cccggcgcgg cctgcgatgt gccctcgcac
      181 ctgtattcat tttcctttga acggcagttc gactggtcgc gcaccttcgc gccccaatcc
      241 gatatcctgc gctacctgcg tcactgcgtg gaccactacg gcctgcgtcc ccgcattcac
      301 accgggaaag aagtcagcag cgcgcgctac gaccgcacca gcgcccgctg ggacgtcgag
      361 tgccacgacg gcagccacta ccgctgccgc gtgctggtcg cggccaccgg tcaactgtgc
      421 cgtccgctga tgccggcact gcccggtctg gaccagttca ggggcgtgct gtttcattcc
      481 gcgcaatggc ggcatgacgt ggatctggcc ggcaagtcgg tggcggtcat cggtaccggc
      541 gccagtgcga tccagtttgt gcccacgctg gcgcggcagg cggcgaagct caccctgttc
      601 cagcgttccc cgccctggat actgcccaag ccggagcgcc tttataccgg cgcctggggc
      661 cgcctgttcc gcgcgctgcc ctggctggtg acgctgcacc gtctctggat ctacatcgca
      721 ctggaatacc gccaaccgat gctgcggggc ttccggccct ggatggcgct ggtggagcgc
      781 atgttccgcc agcacctgga ggagcaggtg tccgatcctg acctgcgtgc ccggctcacg
      841 cccgattatc ccctgggctg caagcgcatc ctgatttccg acgattatta cccggccctg
      901 cagcagccga acgtcacgct ggtgaacgag ccggtggcgc gactggaagc ggatgccatc
      961 gtcagttcat ccggccagcg cttcccggtg gactgcgtga ttctgggcac cggcttcgac
     1021 accacccatt tcctggcacc catccaggtc accggcctgc acggccagac cctggcggac
     1081 acctggaaag acggcgccga agcctacctc ggcctgacag tgcacggttt ccccaacctg
     1141 tttctgctgt atggccccaa caccaacctg ggccataact ccatcgtcta catgctggaa
     1201 agccagttca attacgtgct gtcggcgctg gacgcactgg gccagtaccc gcaaccggcc
     1261 gccgcgctca acgtgcgcgc tgatgtccag caggcattca atcggggttt acagcagcag
     1321 ctggcgaaat cggtgtggtc cggcggttgt cgcagctggt atgtggacgc gcagggacac
     1381 aacgccaaca attggccggg cttcaccttc cgctatcgcg cactgacgcg gcggttgaat
     1441 ccgggtgact atgagtgggt ggggggataa cagattcagc cgttcttcag caaccggccc
     1501 ttctcccggt tccagtcgcg gtcgcgctca gtggcgcgct tgtcgtgtaa ctgcttgcct
     1561 ttggccaggg cgatttcgca tttgacgttg ccattcttcc agtacagcgc cagtggaaca
     1621 caggtcatgc cttgcttggc gatggcgccg aaaatgcgtc ccagctcgcg cttgtgtagc
     1681 agcagcttgc gcgaccgcag cgggtccggc acgaagtggg tcgagacggt cggcaacggc
     1741 gaaatgtgcg cgccgaacag atagccctcg ccatccttca tcagcacata gctgtccagc
     1801 aggttcacct tgccggcacg cagacttttc acttcccagc cctgcagcgc caggcccgcc
     1861 tcgaagcgtt cctcgatgaa atactcgtgc ttggctttct tgttcagggc gatggtaccg
     1921 ccgccatctt tcttgttcga catgagctgt tcgacagaat ccaggctgaa tggctaaaag
     1981 gggcaccgat tataggattt aaccggcgcg ctgtcgtcag cggttttggc cgctgcgata
     2041 aatcgtggga caatggtcaa ttccgttcaa caccgcaagg cagtaaaagg ggaaaacgat
     2101 gggagacagc atccgggtgg aagtggccta cgccctgccg gaccggcagt tggtggtgcc
     2161 gctgcaggtg aaaaagggca ctaccctgtt cgaggccgcc gtgcagtccg gcatcgctac
     2221 caaattcgac ggacttgacc tggaaaaagc cccgatgggc gtgttcggca aggccgagcg
     2281 caagccgaag gagcgcgtgc tggaagacgg cgagcgggtg gaaatctacc gacccctgat
     2341 cgccgacccc aaggaagttc gcaagaaacg cgccgccgcc aaaatggacg acgcggagga
     2401 cgactgagcc ctcgtctcag atgggggcgg ccgggccagc gtcgcggggt tcctcgaccg
     2461 gcgcgggggt gcccatttcg gtggtgttct gcttgacttc gatgcccagc tgcttcatca
     2521 ggtcgttacg cagctgctcc gggtccttgt actcggtgtt gtcgatgcgg gccaggcgct
     2581 cgccctcgaa atacagcacc agatggtttt caatgaaaat gcccttgcc
//
LOCUS       DASUEG010000090         6815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1851619, whole genome shotgun sequence.
ACCESSION   DASUEG010000090 DASUEG010000000
VERSION     DASUEG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851619"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFX11_01500"
     CDS             <1..351
                     /locus_tag="VFX11_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937321.1"
                     /translation="TDYSIKVYAYNHGEHGAAAVMGFITGFTLGVIPSAVTDQYTLVV
                     EASGKDGGKLKDISNNDAVTTWLGLWFIPLMGNSPIEAVSGTLENQLRSALKELVESG
                     ALQYSGVQVPFRNA"
     gene            438..992
                     /locus_tag="VFX11_01505"
     CDS             438..992
                     /locus_tag="VFX11_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937322.1"
                     /translation="MRIHGLSLVAIASLLLTACAIPVAPYSPSYDNVKKLPAPPPTSR
                     IGDFQAANASVGSISVRADHLESPYGKDLVHYLQTALQSEFAKAGLIDDNSHREVTAV
                     IEENDIDTGGISDAHGVIQARFIVKKDGASAYDRQIRIDHHWESSVLAAIANTRAAAA
                     YPKMAQLLLEKLYADEDFIEALKN"
     gene            complement(1068..2066)
                     /gene="zipA"
                     /locus_tag="VFX11_01510"
     CDS             complement(1068..2066)
                     /gene="zipA"
                     /locus_tag="VFX11_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEX4937323.1"
                     /translation="MEIGLREVLILIGFLVVAGILLDGYRRMRRGRLKMDIIQQKEGS
                     SDDAWDLYRSELPNGGARVVPISKSPHHAGDADPDVRNDAGYAEPFGSAQETDDARPE
                     SLGSAYARENSDAGQERKRKPEGDALNQTDLFADDDVLLAARADQQARLAKATRGKGR
                     PQPSEPAPARDKSVADKPVEAPLPDIQEVLVINVMARQGELPGADLQRVLLSCGFRFG
                     EMNIFHRYEQHSGKGAMLFSVANGVEPGTFDLDNMDNFSTPGICMFMQLPGPKRPMHA
                     FEIMMDSARKIANLLEADIRDEQHNILRQQTAEHYRQRVLDFERKMLTMQGGQRQY"
     gene            complement(2226..5729)
                     /gene="smc"
                     /locus_tag="VFX11_01515"
     CDS             complement(2226..5729)
                     /gene="smc"
                     /locus_tag="VFX11_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX4937324.1"
                     /translation="MRLKSIKLAGFKSFVDPTTVAFPDNLSAIVGPNGCGKSNVIDAV
                     RWVMGETSAKHLRGESMTDVIFNGSNTRKPTTQASVELVFDNSDGTLGGEYASYAEIA
                     LKRLVTRDGQSNYYLNSVKCRRKDITDIFLGTGLGPRSYAIIEQGMISRLIEAKPEEL
                     RIYIEEAAGISKYKERRKETEHRMRRTRENLDRLQDLRDELGRQLQHLERQAQAAEKY
                     KQYREEERLAKAQLQALRWKGINDQVEVREARIRDLEVKLEAFVADQRAMDAQIELRR
                     DQHHELSDKLNEVQGRFYGLGADIARIEQTIQHTKERGSQLQKDLQQTIEACKQAEDQ
                     IRLDQEQLEEISIRLAEIEPEYELVKAGADNANENLHEAEERMQEWQLAWDEFNQKAN
                     EPRQRAQVEQSRIQHLEQSVERLRDRIERLKGERETLSAGPLEEEVAILEERMAEADL
                     KLEDLQGTALGYQEQIKQQRERNNQLNLELDQFKNERQTLKGRYASLEALQQAAMGES
                     NLVEWLAQNQLGDKPRLAQQVTAESGWEKAVETVLGDYLQAVCINGLDAVTAVIGSLN
                     KGVVAFLDTGKPVAIPARSGLQPLADKLNGDAAAKNLLAGVFVARDLAEALQLRNQLQ
                     AGESLITQDAIWIGANWMRVARDTAQEDSVLKRHKALEETGARLEEVEAKIESLTETL
                     TDGREGLRELEENREQSQRQFTEFNRSLSDLKAQLSAKQVRLEQFNMRRERISKELDE
                     CREQQQQEQETLGEARLVLQEAMDLMTNDTEQRERLLKQRDENRARLDQVRLAAREGK
                     DKAHQLALQTQQLQTQSTSIKNALERLRQQLSQFKERRTAIEEQLALGDDPFVELQAE
                     LEAKLEARLNVEDELRNARRAQEDCEHDLRASEKKRSEAEQSAQSVRSELERNRMDWQ
                     GMRVQRETLQNQLKEAQYDLETVVAELPPEANEEQWNADLERIAARISRLGPINLAAI
                     DEFKSQSERKNYLDAQHNDLNQALETLENAIRKIDRETRTRFKETFDKVTHGLSTLFP
                     KVFGGGSAYLELTGEDLLDTGVTIMARPPGKKNSTIHLLSGGEKALTAIALVFSIFQL
                     NPAPFCMLDEVDAPLDDANVGRYSRMVEEMSSKVQFIYITHNKIAMEMAHQLMGVTMH
                     EPGVSRLVTVNVEEAAALAIA"
     gene            5981..6766
                     /gene="cysZ"
                     /locus_tag="VFX11_01520"
     CDS             5981..6766
                     /gene="cysZ"
                     /locus_tag="VFX11_01520"
                     /inference="COORDINATES: protein motif:HMM:NF003433.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HEX4937325.1"
                     /translation="MLKEFREGVGYVSEGYRLARRPALRPYMMVPLALNIVLFTISGW
                     LVVYYAYGWIGGLDTVVDLWSWLDWAEGAVNSTLAVLKWVAFAAIILFLLFVMGSSFT
                     MVVHLLVSPFIGVLGEQAEKQLHTPNYPQHTLAQIAWRSIKREIRKLAYWLLRALGLV
                     VLTLILNFIPVVNLAVPVIWFLFGSWVLALQYIDVPADNNGKSFQDVLALMQANRAAV
                     MGFGAVVMALTSVPIVNLFIIPVAVCGGVVFWVRKIQGEAAAS"
BASE COUNT         1157 a         2073 c         1992 g         1593 t
ORIGIN      
        1 accgactact ccatcaaagt ctatgcatat aaccacggcg agcacggtgc ggccgccgtc
       61 atgggcttta tcaccgggtt taccctgggc gtgattccgt cggccgtaac cgaccaatac
      121 acgctggttg ttgaagcgtc cggaaaagac ggcggcaaac tcaaggatat ttccaacaac
      181 gacgccgtca cgacctggct gggtctctgg ttcattccgc tgatgggcaa cagccccatc
      241 gaagccgtct ccggcacact ggaaaaccag ttgcgcagtg cactgaagga actggttgaa
      301 tccggtgcgc tgcagtattc cggagtccag gtgccattcc gtaacgcctg accctcacct
      361 ggttcccacg gccccgcccg cgcaccatca gcgtcgggcc gtggtttttc ttcacaccga
      421 cacaattcga ggcacccatg agaattcacg gcttgtccct ggtggcaatt gcatccctgc
      481 tgttgaccgc ctgcgccatc ccggtcgcgc cctattcacc gtcctacgac aacgtcaaga
      541 aactgcctgc tccaccaccc accagccgca taggtgattt ccaggctgcc aatgccagcg
      601 tgggttccat atctgttcgc gcagatcacc tggaatcacc ctatgggaag gatttggtgc
      661 attacctgca aaccgcactg cagagcgagt ttgccaaagc cgggctgata gacgacaaca
      721 gccatcggga agtgaccgcg gtcattgaag aaaatgatat cgataccggt gggatcagcg
      781 atgcccatgg cgttatccag gcccgcttta ttgtgaagaa ggatggcgcg tccgcctatg
      841 acagacagat ccggatagat caccactggg aatcatccgt gctggctgcg atcgccaaca
      901 cgcgggccgc ggccgcctat cccaaaatgg cgcagctgct gctggagaaa ctgtatgcag
      961 acgaggactt tatcgaagcg ctgaagaact gacggggatt ttttctacgc ggcggctgcc
     1021 ccgatttcac ggtcacagcc gccgcagtca tgattgatgc acggtgttca atactgccgc
     1081 tgcccaccct gcatcgtcag catcttgcgt tcgaaatcca gcacccgctg ccggtaatgt
     1141 tcggccgtct gctggcgcag gatattgtgt tgttcgtcgc gaatgtcggc ttccagcaga
     1201 ttggcgatct tgcgcgcgga atccatcatg atctcgaagg catgcatcgg ccgcttgggt
     1261 ccaggcagtt gcatgaacat gcagatgccc ggcgtgctga agttatccat gttgtccaga
     1321 tcgaaggtgc ccggttccac gccgttggca acgctgaaca gcatggcgcc cttgccgctg
     1381 tgctgctcgt aacgatggaa gatattcatc tcgccgaaac ggaaaccaca ggacagcagg
     1441 acccgctgca gatccgcacc cggtagttcc ccctgccgtg ccatcacatt gatgaccagt
     1501 acttcctgga tatcgggcag aggcgcctcc accggcttgt cggccacgga tttatcgcgg
     1561 gccggtgccg gctcggatgg ctggggccgt cccttgcccc gggtggcttt tgccagtcgt
     1621 gcctgctggt cggcgcgagc agccagcagc acatcgtcat cggcaaacag gtccgtctgg
     1681 ttgagggcat cgccctccgg cttgcgcttg cgctcctgac cagcatcact gttttcgcgc
     1741 gcataggcac tgcccaagga ttccggacgc gcgtcatcgg tttcctgggc cgagccaaac
     1801 ggttctgcgt aacccgcatc attacgcacg tcaggatcag catcaccggc atggtgaggg
     1861 gatttgctga tcggcaccac ccgggcgcca ccattgggca gctcactgcg gtacagatcc
     1921 caggcgtcgt cactgctgcc ttctttctgc tgaattatgt ccatcttcag acggccacgg
     1981 cgcatgcggc gatatccatc cagcaggatg cccgccacca ccaggaagcc gatcagaatc
     2041 agcacttctc tcaggccaat ttccatcaaa ggggtcctct tgtcttccct atgcgccctg
     2101 cgccacttgc gcaatggcaa cgctttgatt cactaatcaa caacatagag caaatccgtc
     2161 aaaaataaag ttgtctgcat caatcttcgg ccaatccggg cattccgggg ccgcttttgc
     2221 cagcctcagg caatcgccag ggccgccgct tcctccacgt tcacggtcac cagacgcgac
     2281 acgcccggct cgtgcatggt cacccccatc agctgatgcg ccatctccat ggcgatcttg
     2341 ttgtgggtga tgtaaatgaa ctgcaccttg gacgacatct cttccaccat gcgcgaatag
     2401 cggcccacgt tggcgtcgtc cagcggcgcg tcgacttcgt ccagcataca aaagggtgcc
     2461 ggattgagct ggaagatgga gaacaccagc gcaatcgctg tcagcgcctt ctcgccaccg
     2521 gacagcaggt gaatggtgct gtttttcttg ccgggcggac gcgccatgat ggtgacgccg
     2581 gtatccagca gatcctcgcc ggtgagttcc agatacgcgc tgccgccgcc gaacactttc
     2641 gggaacaggg tggacaggcc gtgggtcacc ttgtcgaagg tttccttgaa gcgggtgcgg
     2701 gtctcgcggt cgatcttgcg gatggcgttt tccagggttt ccagcgcctg gttcaaatcg
     2761 ttgtgctggg cgtccaggta atttttgcgc tcggattgcg acttgaattc gtcgatggcg
     2821 gcgaggttga tgggacccag gcgggaaata cgcgcggcaa tccgttccag atcggcgttc
     2881 cactgttctt cattggcttc aggcggcagt tcggccacca cagtttccag atcgtattgc
     2941 gcttctttca actggttttg cagggtttcg cgctgcacgc gcatgccctg ccagtccatc
     3001 cggttgcgtt ccagttcgct gcgcacgctc tgcgccgact gttccgcctc gctgcgtttc
     3061 ttctccgagg cgcgcagatc atgttcgcaa tcttcctgcg cacggcgggc gttgcgcagc
     3121 tcgtcttcca cgttcaggcg cgcttccagt ttcgcttcca gttccgcctg cagttccaca
     3181 aaaggatcgt cacccagagc caactgttcc tcgatggcag tacggcgttc cttgaactgc
     3241 gacagctgct ggcgcagacg ctccagcgca ttcttgatcg acgtggactg ggtttgcagt
     3301 tgctgggttt gcagggccag ctgatgcgcc ttgtccttgc cttcgcgggc cgccagccgc
     3361 acctgatcca gccgcgcgcg gttttcatcg cgctgcttca gcagcctttc ccgctgctcg
     3421 gtgtcgttgg tcatcaggtc catggcttcc tgcagcacca gccgcgcttc gcccagggtt
     3481 tcctgttcct gctgctgctg ttcgcgacat tcgtccagtt ccttgctgat gcgctcgcgg
     3541 cgcatgttga actgttccag ccgcacctgc ttggcgctca gctgtgcttt caggtcggac
     3601 agcgaacggt tgaattcggt gaactggcgc tgcgactgct cgcggttttc ttccagttcg
     3661 cgcagacctt cgcggccatc agtgagggtt tcggtgaggc tttcgatttt cgcttcgact
     3721 tcttccagtc gcgcgccggt ttcttccagg gccttgtgac gcttgagaac gctgtcttcc
     3781 tgtgcggtgt cgcgagccac ccgcatccag ttggcgccga tccagatggc atcctgggta
     3841 atcagggatt caccggcctg caattgattg cgcagttgca gtgcttccgc cagatcgcgg
     3901 gccacgaaca cgccggccag caggtttttc gcggcggcat cgccattcag tttgtccgcc
     3961 agcggttgca ggccggagcg cgcaggaatc gcaaccggtt tgccggtatc gagaaacgcc
     4021 accacaccct tgttcaggct gccgataacg gcggtgacgg catccagacc attgatgcac
     4081 accgcctgca gataatcgcc cagcacggtt tccacggcct tttcccagcc gctttccgct
     4141 gtcacctgct gcgccagacg gggcttgtcg cccagctggt tctgcgccag ccattccacc
     4201 agattgcttt cgcccatcgc cgcctgttgc agtgcttcca gcgaggcgta acggcctttc
     4261 agggtctggc gctcgttctt gaactgatcc agttccaggt tgagctgatt gttgcgctcg
     4321 cgctgctgct tgatctgttc ctggtagccc agtgcagtgc cctgcagatc ctccagtttc
     4381 agatccgctt ccgccatgcg ctcttccaga atggcgactt cttcttccag cgggccggca
     4441 gacagcgttt cgcgctcgcc cttcaaacgt tcgatgcgat cccgcagccg ttcgacggac
     4501 tgctccagat gctggatgcg ggattgttcc acctgcgcgc gctgacgcgg ctcgttggct
     4561 ttctggttga attcgtccca cgccagctgc cattcctgca tgcgctcttc cgcttcatgc
     4621 aggttttcat tggcattgtc ggcgccggct ttcaccagtt cgtattcggg ttcaatttcc
     4681 gccaggcgga tggagatttc ttccagctgt tcctggtcaa ggcggatctg gtcttcggcc
     4741 tgcttgcagg cttcgatggt ttgctgcaga tctttctgca gctggctgcc gcgctctttg
     4801 gtgtgctgga tggtctgttc gatgcgggcg atatcggcgc ccagaccgta aaaacggccc
     4861 tgcacttcat tcagcttgtc ggacagttca tggtgctgat cgcggcgcaa ttcgatttgg
     4921 gcatccatcg cgcgctggtc agcgacaaac gcttccagtt tcacttccag atcacggatg
     4981 cgggcttcgc gcacttccac ctgatcgttg atgcctttcc agcgcagcgc ctgcaattgc
     5041 gccttggcca gccgttcctc ttcccggtac tgcttgtatt tttccgcggc ctgcgcctgc
     5101 cgttccagat gctgcagctg gcgccccagt tcgtcacgca gatcctgcag gcggtccagg
     5161 ttttcccgcg tgcggcgcat gcggtgctcg gtttccttgc ggcgttcctt gtatttggaa
     5221 ataccggcgg cttcttcgat gtagatgcgc agttcttccg gcttggcttc gatcaggcgg
     5281 gaaatcatgc cctgctcgat gatggcgtag ctgcgggggc ccaggccggt gccaaggaag
     5341 atgtcggtga tgtccttgcg gcggcatttg acgctgttca ggtaatagtt ggactggccg
     5401 tcgcgggtca ccagacgttt cagcgcaatt tcggcgtagg aagcgtattc accgcccagt
     5461 gtgccgtcac tgttgtcgaa caccagttcc accgacgcct gcgtggtggg cttgcgggtg
     5521 ttggagccgt tgaagatgac atcggtcatg gattcgccac gcagatgctt ggcggaggtt
     5581 tcacccatta cccagcgtac ggcgtcgatg acattggatt tgccgcagcc attggggccg
     5641 acgatggcac tgaggttgtc ggggaacgcc acggtggtgg gatcgacgaa ggatttgaaa
     5701 cccgccagtt tgatcgattt cagacgcatg gatcgttctt tttccttatt ggaatggccg
     5761 gcgcgattgc tcgcttttcg tacaacgatt acccaacgct gcacgcggca taaacgccct
     5821 attgtagcga gttttggcgg cggcaccacc ccggcgggcc aacagttttt caacctataa
     5881 aaacttgatt ttgctgtcaa tccgggcgct tgtggcaggg ttgtgttacc gtttccctcc
     5941 atcctggacc cagtccctgt tcctataagg agaagcccgc atgctcaagg aattccgcga
     6001 aggcgtcggc tatgtcagcg agggttaccg gctggcccgc cggcctgcgc tgcgccccta
     6061 catgatggtg ccgctggcac tgaacattgt gctgttcacc atcagcggct ggctggtggt
     6121 ttattacgcc tatggctgga tcggcgggct ggatacggtt gtggacctgt ggagctggct
     6181 ggactgggcc gagggcgcgg tcaacagcac gctggcggtg ctgaaatggg tggcgttcgc
     6241 cgccatcatt ctgtttctgc tgtttgtcat ggggtccagc ttcaccatgg tggtccacct
     6301 gctggtcagc ccctttatcg gcgttctggg ggaacaggcg gaaaagcagc tgcacacccc
     6361 caactatccc cagcacaccc tggcacagat cgcctggcgc tccatcaaac gggagatccg
     6421 caaactggct tactggctgc tgcgggcgct gggactggtg gtgctgacgc tgatcctgaa
     6481 tttcattccg gtggtgaatc tggcggtgcc ggtgatctgg ttcctgttcg gttcctgggt
     6541 tttggcgctg cagtacatcg acgtgcccgc cgacaacaac ggcaaatcct tccaggacgt
     6601 gctggcgctg atgcaggcca accgggcggc agtgatggga ttcggcgcgg tggtgatggc
     6661 gttgacgtcg gtgcccatcg tcaacctgtt catcattccg gtggcggtgt gcggcggggt
     6721 ggtgttctgg gtgcggaaga ttcagggtga agctgcggcc agctgaagtg gcgcggggag
     6781 cggcaggtcg gcatcaagaa acgctgcaag tacat
//
LOCUS       DASUEG010000091         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1431759, whole genome shotgun sequence.
ACCESSION   DASUEG010000091 DASUEG010000000
VERSION     DASUEG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431759"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFX11_01525"
     CDS             <1..563
                     /locus_tag="VFX11_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937326.1"
                     /translation="QPTNKSPSGITSWADATDQGRLSATFNPSGEHLYLVAGGISREH
                     TKQAHRLMVLNALSLMERHDMGSAFACLAMPGYSAAYESDDYTPEEYLALTHGNGVPR
                     DAFLAFFQDLWPGTHRPLRLLRDGYPPDQRSGGHGACACVDVPDHPAAIHQLFDKLAQ
                     QRIALFGSQSNHPAAQDATDASVVNA"
     gene            560..1468
                     /locus_tag="VFX11_01530"
     CDS             560..1468
                     /locus_tag="VFX11_01530"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4937327.1"
                     /translation="MNASLSTVQTTAAEKRARQQLDEPAPSLRDWPSYLYIGLFAVNS
                     GAIALCNSVWLLAPLVLIQGLFFMGLLELMHQAVHRNFVTGRAGNEVFGTLAGALIGI
                     NMVTYRYFHLEHHRHTCDSADPEGLLYAHSPRTRWSAVAAPIGQAWVAFSINQLAGRY
                     VPAARKPEWQRCRFALLLFMGSLALLAFMAPPVFLAVYLLPFCVFAWIDFFFSQAEHY
                     AAPIRSENEKTDVAQLSYDIRVPRWLSHLMLNRNLHRVHHVWPRTRWFEAPDRLPALH
                     AHEGGRTLSLPAFLTRWMEGGPRLWE"
     gene            1680..3047
                     /gene="eat"
                     /locus_tag="VFX11_01535"
     CDS             1680..3047
                     /gene="eat"
                     /locus_tag="VFX11_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEX4937328.1"
                     /translation="MQAQLKPTLGKLHLWGIAVGLVISGEYFGWSYGWGAAGTLGFLV
                     TTLLVATMYTAFIFSFTELTCAIPHAGGPFAYSLRAFGPRAGLMAGLATLIEFVFAPP
                     AIAMAIGAYLNVQFPALDPKLIAVGAYIVFMTLNILGVKIAATFELVVTVLAILELLV
                     FMGVVSPGFSFSSFVLNGWAGSDTFTLAAIGGIFAAIPFAIWFFLAIEGAAMAAEEAK
                     DPKRTIPVAYVGGILTLVVLALGVMIFAGGVGDWKALSNLNDPLPQAMKTVVGDDSGW
                     LHMLVWIGLFGLVASFHGIILGYSRQLFALARAGFLPAYFARIHPRFHTPHRAIIGGG
                     VVGIGAIYSDSLINLGGMSLTAAMITMSVFGALVMYIMSMLSLFRLRRTAPDMERSFR
                     APLYPFAPALALAIAVICLIAMIYYNPLIAGIFAAFMTIGFVYFQFTQQQRNAAPADA
                     LLASS"
BASE COUNT          527 a          974 c          928 g          698 t
ORIGIN      
        1 tgcaacccac aaacaaatcc ccgtcgggca ttacctcgtg ggccgatgcc actgatcagg
       61 gacgactgag cgcgacgttc aatccctctg gcgaacatct gtatctggtg gccggcggca
      121 tatcccgcga gcacaccaag caggcgcacc ggctgatggt attgaacgcg ctgtcgctga
      181 tggagcggca cgacatgggc agcgcgttcg cctgcctcgc catgccgggc tattccgccg
      241 cctatgaatc cgatgattac acaccggaag aatatctggc gctgacccat ggcaacggcg
      301 taccgcgcga tgcgtttctc gcgttcttcc aggatctgtg gcccggcacg caccgccccc
      361 tgcgcctgct gcgggatggc tatccgccgg accagcgctc cggcggacac ggcgcctgcg
      421 cctgcgtgga cgtaccggat caccccgccg ccatccatca gctgttcgat aaactggcgc
      481 agcagcgcat cgcattgttc ggcagtcagt cgaatcatcc ggccgcgcag gatgcaacgg
      541 atgcgagcgt ggtgaacgca tgaatgcgag cctctccact gtgcaaacca ccgctgcaga
      601 aaagcgggcc agacagcaac tggacgaacc ggcgccttcg ctgcgcgact ggcccagcta
      661 cctgtatatc ggtttgtttg ccgtcaacag cggcgccatc gcgctctgca acagtgtgtg
      721 gctgctggcg ccgctggtac tgatacaggg gctttttttc atgggtctgc tggaattgat
      781 gcaccaggcc gtgcaccgca atttcgtcac cgggcgcgcc ggcaacgaag tcttcggcac
      841 gctggcgggc gcactgattg gcatcaatat ggtgacctac cgctattttc atctggagca
      901 tcaccgccac acctgcgaca gcgccgatcc tgaaggtttg ctgtatgcac acagcccgcg
      961 cacgcgctgg tcggccgtgg cggcgcccat tgggcaggcg tgggtggcgt tcagcatcaa
     1021 ccagctggcg gggcgctatg tgccggcggc acgcaagccg gaatggcaac gttgccgttt
     1081 cgctttgctg ctgttcatgg gcagtctggc actgctggcc ttcatggcac cgccggtttt
     1141 cctcgccgtg tatctgttgc cgttctgcgt gtttgcctgg atcgatttct ttttcagcca
     1201 ggccgagcat tatgccgcgc ccatcagaag cgaaaacgaa aagaccgatg tggcgcagtt
     1261 gtcatatgac atccgtgtac cgcgctggtt gtcccatctg atgctgaatc gcaacctgca
     1321 tcgcgtgcat catgtctggc cgcgcacccg ctggtttgag gcgcccgacc gtctgcctgc
     1381 actgcacgcc catgaaggcg gacgcacgct gtcactgccg gcttttctga cgcgctggat
     1441 ggagggcggt ccccggctct gggaataagg atcagccgcc gcgagacaaa ctttcacggc
     1501 tgccgggttg ctcccgcaca aggcagccgc tttcctgcac gcaccggcaa cgggcacttt
     1561 cctctgcaca aatcacttca ttcccggatt tttccggcac ttgccggccg tttgcgcgct
     1621 ggcacgcatt ctgctggcgt cttctccgcc attccaacac tttcgatgga gaatgcgtca
     1681 tgcaagcaca attgaagccc acattgggca agctgcattt gtggggcatt gcagtggggc
     1741 tggtgatatc cggcgagtat tttggctgga gttacggctg gggcgcggcg ggcacgctgg
     1801 gctttctggt aacgacattg ctggttgcca ccatgtacac cgcgtttatt ttcagtttca
     1861 cggaattgac ctgcgccatt ccgcacgcag gcgggccatt tgcctacagt ctgcgcgctt
     1921 ttggcccccg cgccggactg atggccgggc tggcgacgct gattgaattt gtgtttgcac
     1981 cgccggccat tgcgatggcc atcggcgcgt atctcaatgt gcaatttccc gcgctcgatc
     2041 ccaagttgat cgccgtcggc gcgtatatcg ttttcatgac gctgaatatt ctgggcgtga
     2101 agattgcggc aaccttcgaa ctcgtggtca cggtactggc cattctggaa ctgctggtat
     2161 tcatgggtgt ggtgtcaccg ggcttttcct tctccagttt cgtgctgaac ggctgggccg
     2221 gctccgacac ctttacgctg gcagcgatcg gcggcatatt cgcggccatt ccgtttgcca
     2281 tctggttttt cctcgccatt gaaggcgccg caatggccgc cgaagaagcc aaagatccga
     2341 aacgcacgat tccggtggcc tatgtgggcg gcatcctgac actggtcgta ctcgccctgg
     2401 gcgtgatgat ttttgccggc ggcgtgggtg actggaaggc attgtcgaac ctgaatgatc
     2461 cgctgccaca ggcgatgaaa accgtggtgg gcgacgacag tggctggctg cacatgctgg
     2521 tgtggatcgg actgttcgga ctggtagcgt ctttccacgg catcatcctg ggctattcgc
     2581 gccagctgtt cgcgctggcc cgcgccggat tcctgcccgc ctacttcgcc agaatccatc
     2641 cacgcttcca cacgccgcac cgcgccatca tcggtggcgg cgtggtgggt atcggcgcca
     2701 tctacagcga cagcctgatc aacctgggcg ggatgtcgct gacggcagcg atgatcacca
     2761 tgtcggtgtt cggtgccctg gtcatgtaca tcatgagcat gctcagcctg tttcgtctgc
     2821 gccgcacagc gcccgatatg gaacgcagct tccgcgcgcc gttatatccg ttcgcgcctg
     2881 cactggccct ggcgatcgcc gttatctgcc tgatcgcgat gatctattac aatcccctga
     2941 ttgccggtat ttttgccgct ttcatgacga ttggcttcgt ctacttccag ttcacccagc
     3001 agcaacgcaa cgctgcgcca gcggatgcgc tgctggcaag ctcctgaact ggctgggaaa
     3061 ttgcgcacgg cgggtcagag cgttctggcc cgcccgcagt ctgccgttaa ctcaccgtca
     3121 gattttt
//
LOCUS       DASUEG010000092         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1409822, whole genome shotgun sequence.
ACCESSION   DASUEG010000092 DASUEG010000000
VERSION     DASUEG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409822"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX11_01540"
     CDS             <1..294
                     /locus_tag="VFX11_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4937329.1"
                     /translation="NFLLTIPAMVIVGREITISALREWMAELGKRASVAVSYIGKVKT
                     TVQMIAITGLIANAPDVGNWVVWLAYLLLYAATVLTLWSMLLYLKAAWPDLKE"
     gene            399..474
                     /locus_tag="VFX11_01545"
     tRNA            399..474
                     /locus_tag="VFX11_01545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:432..434,aa:Gly,seq:gcc)
     gene            508..582
                     /locus_tag="VFX11_01550"
     tRNA            508..582
                     /locus_tag="VFX11_01550"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Cys,seq:gca)
     gene            953..2455
                     /locus_tag="VFX11_01555"
     CDS             953..2455
                     /locus_tag="VFX11_01555"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4937330.1"
                     /translation="MSIEYVDVLIIGAGLSGIGAACHLRRDCPNKTFAILEGRNAMGG
                     TWDLFRYPGIRSDSDMYTLGYNFKPWTNPKAIADGASIRDYIRETAAEHKVDRKIRYG
                     HKVIRADWSSDQAQWTVDILRTDSGDTLQMRCNFLLGCTGYYNYAAGFTPHFEGREQF
                     KGTIIHPQHWPEDLDYSGKRVVVIGSGATAVTLVPAMTDKAAHVTMLQRSPSYVASVP
                     LEDKLSNKLRRYLPEKLVYRMARTRNVGFQMGFFKLARSKPDTIKRLLLSQVKRQVGP
                     NVDMAHFTPNYNPWDERLCAVPNGDLFKVIRKGKASVVTDHIERFTENGILLKSGKTL
                     EADIIITATGLDLQLLGGTQLAVDGKPFEMSQTMNYKGVMFRDLPNFAMVFGYTNASW
                     TLKADITCEYLCRLLKLMDRKGARQCTPRAKPGSVHEVPFLDMKSGYVQRALSRLPRQ
                     GSRAPWKLYQNYALDLAMLRFGKIEDDVLEFTRPASARGRSAARVSSATG"
     gene            2690..>3231
                     /locus_tag="VFX11_01560"
     CDS             2690..>3231
                     /locus_tag="VFX11_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937331.1"
                     /translation="MPLKSANMQRLPQERHWLKWWGPVGKISMRWSYWLNQHRVEDVE
                     KTFEGIVQTRRQLLTQWAEQQWAYLRSLARDVEKNWPHVSGPLLATACTLHKEASEIF
                     IVGPGHDVLHSSAPAHEGQRDLNAQACATGMREPFLHGPYPDPLTRTLGATTSSFHDD
                     MTLMFYHPLRVNGAVVGCLCV"
BASE COUNT          644 a          904 c          965 g          718 t
ORIGIN      
        1 aacttcctgc tgacgatccc ggcgatggtg atcgtcggcc gcgagatcac catttctgcc
       61 ctgcgggaat ggatggcgga actgggcaag cgggcgagcg tggcggtgtc ctatatcggc
      121 aaggtcaaaa ccacggtgca gatgatcgcc atcaccggcc tgatcgcgaa cgcgccggat
      181 gtaggcaact gggtggtgtg gctggcctac ctgctgctgt acgcggccac ggttctgaca
      241 ttgtggtcga tgctgctgta tctgaaagcc gcctggcccg acctgaaaga atgatcaaat
      301 cgagccaacc cattgagaac ataacgcttt tatttgggtt gactcgaaag gctttgtggg
      361 tagaataggc aacccacaaa gcgcgagctt ttggtaatgc gggaatagct cagttggtag
      421 agcacgacct tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc tccaggtttt
      481 tcatctcttc ttttgtttca ccccgatggc gcgatagcaa agcggttatg ccgcggattg
      541 caaatccgtt gtaggccggt tcgactccgg ctcgcgcctc catctcctgt ttcctttatt
      601 gcctcagatt ccagcgttga actgtcctgc gcattgcatt cctgtgggta caccgccgtt
      661 ccagcaggca ttaagtcaac tgtgttaatc cgtgacagcc tgcccttatt ttgtcggaat
      721 acacactggc tacctggccc cccggatctg cacaatgtcc cctggatcag gctgcctttg
      781 tccgggcgac aggggctgcc ggcgctgcgg cgcctccgca gtgccagcct gattgctagg
      841 ataattgctg cgctgatgac tgccatctct gcaattgaca gctgaatggc gactgaaatc
      901 ctggcctgat tgcaaaggcc agccgggtta aaacagaagg gttgtttcct atatgtccat
      961 tgaatacgtt gatgttctga tcattggtgc gggtctgtcc ggcattggcg cagcttgcca
     1021 tttgcgccgg gattgtccga acaagacttt cgccatcctg gaaggccgca atgccatggg
     1081 cggaacttgg gatctgttcc gttacccggg catccgctcc gattccgaca tgtacaccct
     1141 gggctacaac ttcaaaccct ggaccaatcc caaagccatt gcggacggtg cttctatccg
     1201 cgattacatt cgcgagacgg ccgcggagca caaggtggat cgcaagatcc gttacgggca
     1261 caaagtgatt cgggcggact ggtccagcga ccaggcgcaa tggacggtgg atatcctgcg
     1321 caccgacagc ggcgataccc tgcagatgcg ttgcaacttc ctgctgggct gtaccggcta
     1381 ttacaactat gcagcgggat tcacgccgca cttcgaaggt cgcgagcagt tcaagggcac
     1441 cattattcat ccgcagcact ggccggaaga tctggattac agcggcaagc gtgtggtggt
     1501 gatcggcagc ggtgccacgg cggtcacgct ggtgccggcc atgaccgaca aggcggccca
     1561 tgtcaccatg ctgcagcggt cgccgagcta tgtggcgtcg gtaccgctgg aagacaagct
     1621 gtccaacaag ctgcgccgct acctgccgga aaaactggtg taccgcatgg cgcgcacccg
     1681 caacgtcggc tttcagatgg gctttttcaa gctggcgcgc agcaagcccg atacgatcaa
     1741 gcggctgctg ttgtcgcagg taaagcgtca ggtgggcccc aatgtggaca tggcgcattt
     1801 cacgcccaat tacaatccgt gggacgaacg cttgtgcgcg gtacccaatg gcgatctgtt
     1861 caaggtgatc cgcaagggca aggcgtcggt ggtgacggat cacatcgagc gcttcactga
     1921 aaacggcatc ctgctgaaat caggcaagac gctggaggcg gacatcatca tcaccgccac
     1981 cggcctggac ctgcaattgc tgggcggcac ccagctggcg gtggacggca agccgttcga
     2041 aatgtcccaa accatgaatt acaagggcgt gatgttccgc gacctgccga atttcgcgat
     2101 ggtgtttggc tacaccaatg cgtcctggac cctgaaggca gacattacct gcgaatacct
     2161 gtgtcgcctg ctcaagctga tggaccgcaa aggggcgcgc cagtgcacgc cgcgggcgaa
     2221 acccggttcg gtgcacgagg tgccgttcct ggacatgaaa tccggttatg tgcagcgggc
     2281 gctgtcgcgg ttgccgcgtc agggttcccg ggcgccctgg aagctgtacc agaactacgc
     2341 actggatctg gcgatgctgc ggttcggcaa gatcgaggac gacgtgctgg aattcacccg
     2401 tccggccagc gcgcgcggcc gaagtgcggc gcgggtgagc agcgcgacgg gttaaggttt
     2461 tccttgttca gtcagtggat tgattgcgcc tgaaaacgca ccattttgat cgcatggcaa
     2521 taaatgtgat taaaaatggt gcgttattct ttttgtcttc ttcatttttc cccgatttat
     2581 ctttccccca taaaaaactc ccgtcttgct ttcgaatttg tccgttcaat gtctattctg
     2641 aattttgaat ggtctttgat tactccgtta gcgaagtgga ttccctgtta tgcctctgaa
     2701 aagcgcgaac atgcagcgtc tgccgcagga acggcactgg ctgaaatggt ggggaccggt
     2761 cggcaagatc agcatgcgct ggtcgtactg gctgaaccag caccgggtgg aagacgttga
     2821 aaagaccttt gaaggcattg tgcagacccg tcgtcagttg ctgacgcaat gggccgagca
     2881 gcagtgggcc tatctgcgca gtctggcgcg ggatgtggaa aaaaactggc cccatgtgtc
     2941 gggcccgttg ctggcgacag cctgcacctt gcacaaggag gcgtcggaga ttttcattgt
     3001 cggtcccggc cacgacgtgt tgcattcgag cgctcccgcc cacgaggggc agcgtgatct
     3061 gaatgcgcag gcgtgtgcca ccggcatgcg cgaacctttc ctgcacggac cctatccaga
     3121 tccgctgacg cgcaccctgg gtgccaccac atcgagtttc cacgacgaca tgacgttgat
     3181 gttctaccac ccgttgcggg tgaatggtgc ggtggtgggc tgcctgtgcg t
//
LOCUS       DASUEG010000093         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_282801, whole genome shotgun sequence.
ACCESSION   DASUEG010000093 DASUEG010000000
VERSION     DASUEG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282801"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFX11_01565"
     CDS             complement(<1..1284)
                     /locus_tag="VFX11_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4937332.1"
                     /translation="MSIRFFLDITPQRLINKELPPHYIRLLAYLAAGITIVAALMTGG
                     LTTSLWWTPLMAVGYPLASHFATGGQRKRNPSTTNSILILVDTIITGGAFVVFDFDPT
                     LCLMLFLLVSAGTITIGGLFGWVVSLCSLLLGIIASLILMGPPTLLFAAPNMPTLVTA
                     LVSSTFYVSVLGYFSFKQANTLIRIQNELTERQKDAASLSRKLSKYLPPQVWGSIFSG
                     QTDVKLQTQRKKLTVFFSDIKGFAEISEELQPEALTDLLNSYFTEMSRIALKYGGTID
                     KFVGDAILIFFGDPASRGHKQDAIACVSMALEMRKRMKLLRQQWRKEGISKPLQVRMG
                     INTGYCTVGNFGAETRMDYTIIGREVNLASRLESVAQPGEILISEATYNLVRDVIMCR
                     DRGQINVKGFSKTVPIFEIVDHRKELGATQSFLECE"
     gene            complement(1482..2201)
                     /locus_tag="VFX11_01570"
     CDS             complement(1482..2201)
                     /locus_tag="VFX11_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEX4937333.1"
                     /translation="MKTHQLHPWNVSVEQARAIQKNLSAWVVTEGRCRNPRLLGRIEM
                     ACTHENHNLATVQASVSIRSLPSLQLLERKVSIRTSEFPAVEGLASFRKVPAVVAALA
                     KLVRVPDVIICDGRGRTGSNSFGVASHVGLITNLPTIGIRAPRPRLLSDLLGNARGSW
                     LPVIDGGVEAVLLRVIEGLDPLMVSPAHRIDLEDAMRQVLKFVPASTPAQEYLQLLYP
                     EAGIRGKLTTPLTLVSSRRPA"
     gene            2431..2991
                     /locus_tag="VFX11_01575"
     CDS             2431..2991
                     /locus_tag="VFX11_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX4937334.1"
                     /translation="MSDVIHAIQNRISIPRLIAPAPGPEQLRTLFSCAVRAPDHGRMR
                     PWRFVAMQGEALVRLGLAFEQAGLAGDPGADDSKRARWRDMPLRAPMVIAAIARIRPN
                     PKVPDWEQVVAVATAVQNIQLAARELGFGTMWRTGEMTVAPAVLEHLEMAATDRIVAF
                     LYIGTPEGDARAPEFQPLDECVEYRS"
     gene            3105..3195
                     /locus_tag="VFX11_01580"
     tRNA            3105..3195
                     /locus_tag="VFX11_01580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3139..3141,aa:Ser,seq:gga)
BASE COUNT          646 a          927 c         1040 g          667 t
ORIGIN      
        1 ctcgcattcc aggaaactct gggtggcgcc cagttccttg cgatgatcca cgatttcgaa
       61 aatcggtaca gtcttgctga agcctttcac gttgatctgg ccccggtcgc ggcacatgat
      121 cacgtcgcgc accagattgt aggtggcttc ggaaatcagg atctcgcccg gttgtgccac
      181 cgattccagc cggctggcca ggttcacttc gcggccgatg atggtgtaat ccatccgtgt
      241 ttccgcgccg aagttgccca cggtgcaata accggtgttg atgcccatgc gcacctgcaa
      301 cggtttgctg atgccttcct tgcgccactg ctgccgcagc agtttcatgc gcttgcgcat
      361 ttccagcgcc atcgacacgc aggcaatggc gtcctgcttg tggccgcgcg acgccgggtc
      421 gccgaagaaa atcagaatcg cgtcgcccac gaatttgtcg atggtgccgc catatttcag
      481 cgcgatgcgt gacatctcag tgaagtagct gttgagcaga tcggtcagcg cttccggttg
      541 caattcctcg gaaatctcgg caaagccctt gatgtcggaa aagaacacgg tgagtttctt
      601 acgctgggtt tgcagcttca catcggtctg gccggaaaag atcgagcccc acacttgagg
      661 cggcagatac ttggacagtt tgcgcgacag gctggcggcg tccttctgcc gctcggtcag
      721 ttcgttctgg atgcggatca gcgtattggc ctgcttgaac gagaaatagc ccagcacgct
      781 gacataaaaa gtggacgaca ccagtgccgt caccagtgtg ggcatgttgg gcgccgcaaa
      841 cagcagggtg ggcgggccca tcaggatcag gctggcaatg atgcccagca acaggctgca
      901 caggcttact acccagccga acaggccgcc gatggtgata gtgccggcgc tgaccagcag
      961 gaacagcatc aggcagagcg tgggatcgaa atcgaatacc acgaaggcac cgccggtaat
     1021 gatggtgtcc accaggatca ggatggaatt ggtggtggac ggattcctct tgcgttgccc
     1081 gccggtcgca aagtgggatg ccagcggata cccgaccgcc atcagcggtg tccaccacag
     1141 gctggtggtc aggccgccgg tcatcagggc cgcgacaatg gtaatgcccg ccgccagata
     1201 ggccagcaag cgaatgtagt gggggggcag ttccttgttg atcaggcgtt gtggcgtaat
     1261 gtccaggaaa aacctaattg acatgcaacg tcttccagag cgtcgaaacc attggtttca
     1321 tgagtttcct gccaaatgga tatgttcgga acactgcgtc ttttacctgt aaaaaacggc
     1381 aggttattca acagtgtaag ccgaacatcc gggggctaac aacggcatcc tgcgtgattt
     1441 tttgtggttc gcgtttttgt gttttgggtt ctgcggcgcc ctcaggcggg gcgccgggac
     1501 gaaaccaggg ttagcggcgt ggtcagtttg ccacggatgc cggcctccgg atacagcagt
     1561 tgcaggtatt cctgcgccgg cgtgctggcc ggcacgaact tcagaacctg gcgcatggcg
     1621 tcttccagat cgatgcggtg ggcaggggac accatcagcg ggtccagccc ctcgatcacc
     1681 cgcagcagaa ccgcttccac gccgccgtcg atgaccggta gccagctgcc gcgggcgttg
     1741 cccagcaggt cgctcagcag gcgggggcgg ggtgcgcgta tgccaatggt gggaaggttg
     1801 gtgatcaggc ccacgtggga tgcgacgccg aaactgttcg acccggtgcg gccgcgcccg
     1861 tcgcagatga tgacatccgg cacccgtaca agtttggcca gggcggccac cacggccggc
     1921 accttgcgaa aggatgccag cccttccacg gccggaaatt cgctggtgcg gatcgacacc
     1981 ttgcgttcca gcagctgcag tgatggcagc gaccgtatcg aaaccgacgc ctgcacggtc
     2041 gccaggttgt ggttttcgtg ggtgcaggcc atttctatcc ggcccagcag gcgcggattg
     2101 cggcagcggc cctcggtcac aacccaggcg ctgaggtttt tctggatggc acgggcttgc
     2161 tcaacactga cattccatgg atgtagttga tgcgttttca tgcagaaatc ccgacagggc
     2221 atgtggtagt tggttaaagt atcgacaaca tttggtaaca gaagcgagaa atagatcatg
     2281 ttttgaacaa aacggcgtca aattttatac agtccggctg gaaggaagga aacccaggat
     2341 gagcggttac tggtttgcgg gatttggata agcgcttgtc tgggctatca tttcgcttct
     2401 gttcacaacc tctcccagcc caggtgcacc atgtcggacg ttatccacgc catccagaac
     2461 cgtatttcga tcccccgcct gattgccccg gcccccggcc ctgagcagct gcggaccctg
     2521 ttttcgtgcg ccgtgcgggc accggatcat ggtcgcatgc ggccgtggcg gtttgtggcg
     2581 atgcaggggg aggccctggt ccggctggga ctggcgttcg aacaggccgg actggcgggc
     2641 gacccgggcg ccgatgacag caagcgggcc cgctggcggg acatgcccct gcgggcgccg
     2701 atggtgattg cggcgattgc ccgtatccgg cccaatccca aggtacccga ctgggagcag
     2761 gtggtggcgg tggcgacggc cgtacagaac attcagctgg cggcgcggga actgggtttc
     2821 ggcaccatgt ggcgcaccgg ggagatgacg gtggccccgg cggtactgga acatctggag
     2881 atggcggcaa ctgaccggat tgtggcattc ctttatatag gaacgccgga aggagacgcc
     2941 cgggcaccgg aattccagcc cctggatgag tgcgtcgaat accgtagcta gcgcccgcaa
     3001 ttaacccagg ctgcaggatt gaaatttccc cccgaacgct atatcttacg cgcctcttgt
     3061 gtgagcgctg gccggttggc ccgggctgca cagattccag atccggtgag gtgtccgagc
     3121 ggttgaagga gcacgcctgg aaagtgtgtg tacgttaata gcgtaccgag ggttcgaatc
     3181 cctccctcac cgccatataa acgaaaaagc cccggacaca atgccggggc tttttcgttt
     3241 atatgacggt gagggagggg ccagacgccg cagcgggttc
//
LOCUS       DASUEG010000094         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2030714, whole genome shotgun sequence.
ACCESSION   DASUEG010000094 DASUEG010000000
VERSION     DASUEG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030714"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFX11_01585"
     CDS             <1..1300
                     /locus_tag="VFX11_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4937335.1"
                     /translation="QVAQIEQVRNQAYVDSVTGLGNGRFFNAQLQSRLESPEEPFSGV
                     LVRLQVKGMGEYNEHYGHESGNLLLRRIAQIWRDQLQEVEGHCVARVAGARFAALLPH
                     LSQDMAQQKLKHAMESIQLLDAINQGGASVQVFAGMASCRLGNDPQLLEAQASAALER
                     AQNLRSGHLEIYDESAHGDAMAPNLPGITNWAAFLRDVIDHKRVVLHYQPVMSCVDQS
                     LMHYEVLARVSVEGKLVTAGHFIPLVERFAMIADFDRMVIRLLIEHLKSQEQGTRVPL
                     AINLSSHSIRTEGFDDWVLDAVELNPDIAPYLIFEVPELTVRTAHGKLRRLAAGLKEV
                     GAKLTIDHFGTTNSSFGYLSGLPLYSIKVDNSYIRDIERNLDHQFFVQSLVRIAHSRQ
                     ILLTAEMVEGIEQWNLLRRFQLDGAQGYFLGEPQAARAAA"
     gene            1367..>2055
                     /locus_tag="VFX11_01590"
     CDS             1367..>2055
                     /locus_tag="VFX11_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX4937336.1"
                     /translation="MSTQENKGLVAKIVLGIVGIYLLFATGLGVYWSDEPEIWNVNSA
                     VEQDLVPYNAPRAPGFATTSTLIRLADLLLEKPGGYLTNDITPPGLWLDNIPSWEYGV
                     LVQIRDLTRSLRKDIARSQSTSVENAELAIAEPQFNFDSSSFWFPSSESEYRRGILYL
                     KKYQKSLVIQETNSGQFYARADNLRNWLADVETRLGSMSQRLSASVGKEQLNVDLAGD
                     SAATQSATAPS"
BASE COUNT          404 a          626 c          661 g          364 t
ORIGIN      
        1 gcaggtggcg cagatcgagc aggtgcgtaa tcaggcttat gtggattctg tcaccggcct
       61 gggcaacggc cgctttttca atgcccaatt gcagtcgcgg ctggaatccc cggaagaacc
      121 cttcagcggc gtgctggtgc ggctgcaggt gaaggggatg ggggagtaca acgaacatta
      181 cggccacgaa tccggcaatc tgctgctgcg gcgcattgcc cagatctggc gcgatcagtt
      241 gcaggaagtg gaaggccact gcgtggcgcg ggtggcgggc gcccggttcg ctgcgctgct
      301 gccgcacctg tcccaggaca tggcgcagca gaaactgaag cacgcaatgg agagcatcca
      361 gttgctggac gccatcaatc agggcggtgc cagcgtgcag gtgtttgccg gcatggccag
      421 ctgccggttg gggaatgatc cccagttgct ggaggcccag gccagcgcgg cgctggagcg
      481 ggcgcagaac ctgcgcagcg gccatctgga aatctacgat gaatccgccc acggcgatgc
      541 gatggcgccc aatctgccgg gcatcaccaa ctgggcggcc tttttgcgcg acgtgatcga
      601 tcacaagcgc gtggtgctgc actaccagcc ggtgatgtcc tgcgtcgatc agagcctgat
      661 gcactacgaa gtgctggcgc gggtatcggt ggagggcaag ctggtcaccg ccggccattt
      721 catcccgctg gtggagcgct tcgccatgat cgcggacttc gatcgcatgg tgatccgtct
      781 gctgatcgaa cacctgaaat cccaggagca gggcacccgg gtgccgctgg ccatcaacct
      841 gtccagccac agcatccgca cggaaggctt cgatgactgg gtgctggacg cagtggaact
      901 gaatcctgac atcgcgccct atctgatttt cgaagtgccg gaactcaccg tgcgcaccgc
      961 ccatggcaaa ctgcggcggc tggcggcggg cttgaaagag gtgggcgcca aactcaccat
     1021 cgaccatttc ggtaccacca acagcagttt tggctatctg agcggcctgc cgctgtattc
     1081 catcaaggtg gacaattcct acatccgcga catcgagcgc aatctggatc accagttttt
     1141 cgtgcagtcc ctcgtgcgca tcgcccacag ccgccagatc ctgctgacgg cggaaatggt
     1201 ggaaggcatc gagcaatgga atctgctgcg ccggtttcag ctggacggcg cgcagggata
     1261 cttcctgggc gagccccagg ccgcccgcgc ggctgcctga acgccttgcg ggccagggcc
     1321 taaacaggta cggttacggg caggaatcac tgcagaggat acgagcttga gcacacaaga
     1381 aaacaaaggc ctggtggcga aaatcgtcct gggcatcgtg gggatctatt tactgtttgc
     1441 gacaggcctg ggcgtttact ggagcgacga gcccgagatc tggaatgtga attcggcggt
     1501 cgagcaggat ctggtgccgt acaacgcgcc gcgggccccc gggttcgcca cgacgtccac
     1561 cctgatccgt ctggcggacc tgctgctgga aaagcccggc ggctacctga ccaacgacat
     1621 cacgccgccg ggcctctggc tggataacat tcccagctgg gaatacggcg tgctggtgca
     1681 gattcgcgac ctgacccgct ccctgcgcaa ggacatcgcg cggtcgcagt ccacctcggt
     1741 ggagaatgcc gagctggcca tcgccgaacc ccagttcaat ttcgacagca gcagcttctg
     1801 gtttccctcc tccgaatcgg aataccgccg cggcatcctg tacctgaaga aataccagaa
     1861 gagcctggtg atccaggaaa ccaactccgg ccagttctat gcccgggccg ataacctgcg
     1921 caactggctg gcggacgtgg aaacccgcct gggcagcatg tcgcagcgcc tgagtgccag
     1981 cgtgggcaag gaacagctga atgtggatct ggcgggtgac agcgcggcga cgcagtccgc
     2041 caccgcgccc agcga
//
LOCUS       DASUEG010000095         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1144413, whole genome shotgun sequence.
ACCESSION   DASUEG010000095 DASUEG010000000
VERSION     DASUEG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144413"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFX11_01595"
     CDS             <1..483
                     /locus_tag="VFX11_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937337.1"
                     /translation="AEFSSFDNLSFTVEGDLNEAELASLNDLFGQVNDVAETFYGGNI
                     EEAFNQAMDVGMDPEQLASFAVNMNQTEAVAVRSTYAAVQNMAGPARTNPFEDLFQRL
                     GDFASQVQESSDVVAGIESAIENLQSLYTDLLTRLHPGRGEDNGRGNDFQRFMNRLTS
                     "
     gene            619..>4509
                     /locus_tag="VFX11_01600"
     CDS             619..>4509
                     /locus_tag="VFX11_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937338.1"
                     /translation="MIGRAILGMMLWWITQLALAVTVPVPDALQPWKDWALFGEETAR
                     CPFYYQDFANKQCQWPGQLQLSVEPGGLRFEQDWRLYSESTLALPGSEGFWPGAVTVN
                     GKPATVVEQNNRPFVRLPAGQFRLAGLISFSTKPDSLLIPAQTALVQLKQQGVAVPNP
                     RIDADGRLWLVERQNAEPTDTGASEDKLNIRVFRHLVDDIPFRLETLIELEVGGKPRE
                     MQLGKVLFTDFTPVSFTSPLPARIDADGTLRVQVRPGQWTLRIDAHRNGAVQQLTLET
                     VADKLWPEDEIWVFETLPDLRQIRVEGVDSIDPSQTSLPGEWQQFPAYRLQAGNSMTF
                     AELRRGDPQPSPNQLTLERQFWLDFDGAGLTVRDRFSGNLYRDWRLTAQPELQLGSLE
                     LNGAPQVITTLETEAGKTQGVEVRSGSLQASALSRMAETGGLHVRSLSSTGWQHDVDQ
                     LNATLQLPPGWRVFTVTGVEQAMNTWTGSWNVWDIFIVLIAIVAVIRLRGIVPGAICA
                     LALVLIYPEASEFLYLWLNVIAVMAILFFLPEGKLRTTLRWYGSASALLLALWFLSFM
                     VDQARLGLYPQLQQPWTQMGQGTSYYGGYGENQQYDMATAVAPASAPMEMAMDAAMEA
                     GAGAEQESYRSLAKLSSPRSAPRKPAPNIERSDPNLAAQTGPGQPNWEWMQTTLYWNG
                     PVTQGEQFSVYLLTPWENRLLAWLRVILGVAMLASVLGLQKIQGHWKIAGWRPRAVAS
                     AAALVLMAVLLPAAPNANAADIPDEKMLETLKKRLLEERDCTPACLGIERTHLQLNGN
                     RLTVRFSVHAQQDLFWPLPDSQQQWRIDSVQLDDQPHVARKLQGSLQLPVSAGTHTLQ
                     LSGTVNPQREFQLTFPVMPHNLTVSADGWDVRGLDNGRLQGNGLYFYPAAREAQTADA
                     EEKQLTPDPVPPFVQVQRELRLGLNWYLETTVQRIAPQQGGITLEIPLLPGESITTQE
                     IEVNAGKARIQLKPGQDAIAWTSTLDKTNTLTLQAGENAQWSEHWSVDPSPLWHISVD
                     GIAPVKETGVVGQWRPRWRPYPGEKIVLNIVRPEAVAGATTTIDQVQQTWTPGLRESR
                     SVVKLHLVSSKGSEQHIELPEGAQVKQVTVDDEARAADEDSNKVIVPVNPGQHWIEVE
                     WRQPEEHSVRVKTPAVSIGAPYVNHGIQVEMPRDRWVLFAGGPAMGPAILFWGVTLVT
                     LLVAVVLGRFRQLPLKTVHWVLLVLGLCAGWIQAMIPIALWFFAMHRRSQLNPQKITR
                     TQFNLLQLFLAGFSLFVFAVLIAAIPNGLLGSPDM"
BASE COUNT          898 a         1492 c         1365 g          754 t
ORIGIN      
        1 gccgagttct ccagcttcga caacctgtcc ttcaccgtgg aaggcgatct gaacgaagcc
       61 gaactggcgt cgctgaacga tctgttcggt caggtgaacg atgtggcgga aaccttttac
      121 ggcggcaata tcgaggaagc attcaaccag gccatggacg tgggcatgga cccggaacaa
      181 ctggccagct tcgccgtgaa catgaaccag accgaagccg ttgcggtgcg cagcacctat
      241 gcggcggtgc agaacatggc tggcccggcc cgcaccaacc cgttcgaaga cctgttccag
      301 cgcctgggtg attttgccag tcaggtgcag gaatccagtg acgtggtggc cggcatcgaa
      361 agcgccatcg aaaacctgca atccctgtac accgatctgc tgacccggtt gcatccgggc
      421 cgtggcgaag acaacgggcg tggcaacgat ttccagcgct tcatgaaccg ccttaccagc
      481 tgatcgatcc gttttaccct gcagcttttt tgttgcaagg cagcccgcac cgaatttaac
      541 ccggcgcggg ctgttttttt tcagccactc cgtttatgtt gcaattcgtt ccccgcttaa
      601 cgaactggag atggatgcat gatcgggcgc gcaatactgg gcatgatgct gtggtggata
      661 acgcaactgg cgctggcggt gaccgtgccg gtgccggatg cactgcaacc ctggaaggac
      721 tgggcactgt tcggcgagga aaccgcccgc tgtccgtttt actaccagga cttcgccaac
      781 aagcaatgcc agtggcccgg ccagctgcaa ctgagcgtgg aacccggcgg cctgcgcttc
      841 gaacaggact ggcggctgta cagcgaaagc acgctggcgc tgccggggtc ggaaggcttc
      901 tggcccggtg cggtgacggt gaacggcaaa cccgccacgg tggtggaaca gaacaaccgc
      961 cccttcgtgc ggctgccggc gggacaattc cgcctggcgg gcctgatcag cttcagcacc
     1021 aaacccgatt cactgctgat tcccgcgcaa accgcgctgg tgcagctgaa acagcaaggc
     1081 gttgcggtgc ccaatccgcg catcgacgcc gacggccgcc tctggctggt ggagcgccag
     1141 aacgccgaac ccaccgacac cggcgccagc gaagacaaac tcaacatccg cgtcttccgg
     1201 catctggtgg acgacattcc cttccgcctg gaaaccctga tcgaactgga agtgggcggc
     1261 aagccccgcg aaatgcagct gggcaaggtg ctgttcaccg atttcacgcc tgtctcattc
     1321 accagcccgc tgccggcccg catcgacgcc gacggcacat tgcgcgttca ggtgcgcccc
     1381 ggccagtgga ccctgcgcat cgacgcccac cgcaacggcg cggtgcaaca actgacactg
     1441 gaaaccgtgg cagacaaact ctggccggaa gacgaaatct gggtgttcga aaccctgccg
     1501 gacctgcgcc agatccgcgt ggaaggcgtc gacagcatcg accccagcca gacctctctg
     1561 cccggtgaat ggcaacagtt tcccgcctat cgtttgcaag ccggcaacag catgaccttc
     1621 gcggaactgc gccggggcga tccgcaaccg tcgcccaacc agctcacact ggaacgccaa
     1681 ttctggctgg atttcgacgg cgccggcctc accgtgcgcg accgtttcag cggcaacctg
     1741 taccgcgact ggcgcctcac cgcccagccc gaactgcaac tgggcagcct ggaactgaat
     1801 ggcgcgccgc aggtgatcac cacgctggaa acggaagcag gcaaaaccca gggcgtggaa
     1861 gtgcgcagcg gatcattgca ggccagtgcg cttagccgca tggcggaaac tggcggcctg
     1921 cacgtgcgca gcctcagctc caccggctgg cagcacgacg tggatcagct caacgccacc
     1981 ctgcaactgc cgccgggctg gcgcgtgttc accgtcaccg gcgtggagca ggccatgaac
     2041 acctggaccg gctcctggaa cgtgtgggac atctttatcg tactgatcgc catcgtcgcc
     2101 gtgatccgtt tgcgcggcat cgtgccgggt gcgatctgcg cgctggcgct ggtgctgatc
     2161 tatcccgagg cgtcggaatt tttatacctg tggctgaacg tgatcgcggt aatggcgatc
     2221 ctgttcttcc tgccggaagg caaactgcgc accacgctgc gctggtatgg ctcggcgtca
     2281 gccttgctgc tggcgctgtg gtttttgtcg ttcatggtgg accaggcgcg gctgggcctg
     2341 tacccgcaac tgcaacaacc ctggacacag atggggcagg gcacgtcgta ttacggtggc
     2401 tacggtgaaa accagcaata tgacatggcc accgccgttg ctcccgcctc tgcaccgatg
     2461 gaaatggcga tggatgccgc gatggaagcg ggcgccggcg cggagcagga atcctatcgt
     2521 tccctggcca agttgtcgtc gccacgctcg gcaccacgca aaccagcgcc caacatcgaa
     2581 cgctccgacc ccaacctggc cgcgcaaacc ggccccggcc agcccaactg ggaatggatg
     2641 cagaccacgc tgtactggaa cggccccgtc acccagggcg aacagttcag cgtctacctg
     2701 ctcaccccgt gggaaaaccg cctgctggcg tggctgcgcg tgatcctcgg cgtggccatg
     2761 ctcgccagcg tgctgggcct gcagaaaatc cagggccact ggaaaatcgc cggctggcgt
     2821 ccgcgcgctg ttgcatccgc cgccgcgctg gtgctgatgg ccgtgctgct gcctgccgca
     2881 cccaacgcca atgccgccga catccccgat gaaaagatgt tggagacatt aaaaaaacgc
     2941 ctgctggaag aacgcgactg cacgccggcg tgtctgggta tcgagcgcac ccacctgcaa
     3001 ctgaacggca accgcctcac cgtgcgtttc agcgtgcacg cgcagcagga tctgttctgg
     3061 ccgctgcccg acagccagca gcaatggcgc atcgacagcg tgcaactgga cgaccagccc
     3121 cacgtcgccc gcaaactgca aggcagcctg caactgccgg tcagcgccgg cacgcacacg
     3181 ctgcaactgt cgggcaccgt caacccgcag cgggaattcc agctcacctt cccggtgatg
     3241 ccgcacaacc tcactgtcag cgccgacggc tgggacgtgc gcggcctcga caacggccgc
     3301 ctgcaaggca acggcctgta cttctacccg gccgcacgcg aagcacaaac cgccgacgcc
     3361 gaggaaaaac aactcacacc cgatccagtg ccaccgttcg tgcaggtgca acgcgaattg
     3421 cgcctgggcc tgaactggta cctggaaacc accgtgcagc gcatcgcgcc gcagcagggt
     3481 ggcatcaccc tggaaattcc gctgctgccg ggggaatcca tcaccacgca ggaaattgaa
     3541 gtcaacgccg gcaaggcgcg catccagctg aaaccgggcc aggatgcgat tgcgtggacc
     3601 tccactctcg acaaaaccaa cacgcttaca ttgcaggccg gtgaaaatgc gcaatggagc
     3661 gaacactgga gcgtcgatcc ctcgccgttg tggcacatca gcgtcgatgg cattgcaccg
     3721 gtgaaggaaa ccggcgtggt ggggcagtgg cgtccgcgct ggcggccata ccccggcgaa
     3781 aaaatcgtgc tcaacatcgt gcggcccgaa gccgtcgccg gcgccaccac caccatcgat
     3841 caggtgcagc aaacctggac cccgggcctg cgcgaatccc gcagcgtggt gaaactgcat
     3901 ctggtgagca gcaagggcag cgagcaacac atcgaactgc cggaaggcgc gcaggtaaaa
     3961 caggtcaccg tggatgacga agcccgcgcc gccgacgaag acagcaacaa agtgatcgtg
     4021 cccgtgaacc ctggccagca ctggatcgaa gtggaatggc gccagccgga agaacattcc
     4081 gtgcgcgtga aaacaccggc cgtttccatc ggcgcgccgt acgtgaacca cggcattcaa
     4141 gtggaaatgc caagggaccg ctgggtgctg ttcgccggcg gccccgccat gggcccggcc
     4201 atcctgttct ggggcgtcac cctcgtcacc ctgctggtgg ccgtggtgct gggccgcttc
     4261 cgccagttgc cgctgaaaac cgtgcactgg gtgctgctgg tgctgggcct gtgcgccggc
     4321 tggattcagg ccatgattcc catcgcactc tggtttttcg ccatgcaccg gcgcagccag
     4381 ctgaacccgc agaaaatcac ccgcacccag ttcaacctgc tgcaactttt tttggcgggc
     4441 ttcagtctgt ttgtgttcgc cgtgctgatc gctgcgatcc ccaacggcct gctgggcagc
     4501 cccgacatg
//
LOCUS       DASUEG010000096         7720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1497772, whole genome shotgun sequence.
ACCESSION   DASUEG010000096 DASUEG010000000
VERSION     DASUEG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497772"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFX11_01605"
     CDS             <1..1051
                     /locus_tag="VFX11_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX4937339.1"
                     /translation="ILDEQNLVRELEPLRNLPSSTQDGHHISLMVNTGLLADVQRSLG
                     RGAEGVGLYRTEIPFMVRDRFPSELEQMEIYRQQLEAFHPASVTMRTLDIGGDKALPY
                     FPIHEDNPFLGWRGIRVTLDHPEIFLVQIRAMLWASESLDNLRIMLPMVSSITEIEEA
                     MHLIHRAYLEVLEEGAHVVMPQVGVMIEVPAAVYQCREFVQQCDFVSVGSNDLTQYIL
                     AVDRNNRRVANLYHAFHPAVLRALQTVVTDVKAEGKPVSICGELAGDPRGAILLMAMG
                     YDTLSMSASSLLRVKWVLRKIGLARAKEILQQVWTMDNAQVIRSHMDLMMERAGLGSF
                     IRPMHFPGPAKEPTV"
     gene            complement(1123..1710)
                     /locus_tag="VFX11_01610"
     CDS             complement(1123..1710)
                     /locus_tag="VFX11_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEX4937340.1"
                     /translation="MFRMSAFALLAVLALQPLSSHAEMSQAELQQILKPRIDGLMFLT
                     KNAMILDAIREQNRANASLESIQKIDEEWKAGKSPLIQELQENKVGAFLKNIIVQQSD
                     VYNEAFVTDAKGANVAAFPATSDYWQGDEDKFTKAFAGGKGEVFIGDVEEDESTKQTA
                     VQISVPMLYNKEAIGVLVIGVKVSVLEAEKLKAGK"
     gene            complement(1833..2375)
                     /locus_tag="VFX11_01615"
     CDS             complement(1833..2375)
                     /locus_tag="VFX11_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937341.1"
                     /translation="MTTIPALLLIDDDSLFLQTLGRSLQRRGTEVATARDGTSALQIL
                     QHKRFDLILLDLNLDGESGLKLLQQILALQPDARVVMLTAYASVATAVDAIKRGAENY
                     LCKPVTAADILALLQDDADNTTDTNPLQENPLSVDRLEWEHLQRVLLEHNGNISATAR
                     ALNMHRRTLQRKLQKRPVSK"
     gene            complement(2372..3700)
                     /locus_tag="VFX11_01620"
     CDS             complement(2372..3700)
                     /locus_tag="VFX11_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4937342.1"
                     /translation="MKQPATHASASWWQAPLQRDSLRYLAWYMCILLAVMLTASLAFH
                     LQQISLPARLHWLLLPSQALLTAVTFIAWRRRASVPVALYLLLLFCSIGLLGWYLHDA
                     GGHTNPVISLLLVPLAMGAALLGWQATALIALSVLLLYTALTEYFIPLEMISNMAGDT
                     ASHAHHNHAGASDHFMQLHLLGMWITFAVSVLLIVGLVLPLAQSMRRQRDQIARQQEK
                     ILQDEKLIAMATFAASAAHHLGTPLSTLAVLTDDLKDALTDRPDLQQDLQLMAQQIHT
                     CKATLHGMMHKADNLRNDVREPVAIDLLTQRLRQQFNLLHPARELRVIGQPPSGTVVG
                     DETLDQALLNLLDNAARVSATDPELAFEQQGNIICLRITDTGPGIPDAVRPQLGEAFV
                     TSRQDGLGLGLFLSHATINRLGGTLRLLPSTRASGTVTEVRLPLAGTETP"
     gene            complement(3800..6865)
                     /locus_tag="VFX11_01625"
     CDS             complement(3800..6865)
                     /locus_tag="VFX11_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4937343.1"
                     /translation="MELSAVILLTFNFLYIGATPAFFFRKDGSFNLMWFITGLPLFLW
                     PVSLFGVYWGYLEPNLSLDSEMYASMKTLGVLLSCGSIALISLTMGTHRIPLALWHQD
                     NDAPRTIVTWGAYRFIRHPFYTSFIIAHTAALMIAPHWFQLLLLTYQILILNHTAARE
                     ERNLSQSSFGAEYRDFITRTGRFFPKPNAAAFMETYLVWVTQYRHLVLGLLAGFTIFF
                     SVFLVNIGINATPYFISPDHPGRVTESEIKGIFTNTEEQAFVALVDEKNGIFTTANLE
                     LVQKLTQQLERISFADDTDLERLRSIAGDDTEAQAIVATVAQNGIGLEDVEPLRQLVQ
                     HGKSAKTLTTQELEKIEDIIVRARPVKRVRSLFTVEDIRVDGDDLAIDSLIEQIPSDS
                     AGLQALQQHIMANPLLVGVIVSQDGKATNMQVELNIAEDDSPNMQAVYAAIEKLLAEN
                     GGDAQLHFSGPPMVTAQIAQIIQQDNMLFFPLVSTVICLILFISFRRLQAVVLTMTVC
                     TLSTLWTMGFMAMFGIDVNIVTASLPVFLMTIAVADSIHYLTEYYGLLNTHDRVEAVK
                     VALRHLMKPMFMTSLTDFFGFIALAYTSLVFVREFGIFIAAGAVFAFIISVTLLPAIL
                     PLLKQPANASENKQSPLMNPVNRGFGKLYLTLGRAPLLVLAATAAIAAFTTYLSSEVR
                     VDNHNIVQFSEDTRIRQDDAILNRHFGGTVPINVWFSAADERRFTQPDVIIAMDQIAQ
                     RFEQHDIIGYVGSPSNLVKRIHQLLNNTDYALPADMSPELIAQYFLLYENGNGQEIRD
                     SLDENYRNARLVALSHTDQSSKVRAVLQDVQAYARTVLPADVKMQVAGFGQIMVTSTD
                     EIVNGQISSLTIATLLIAGVMILLFRSWWTGVLSVIPLALTILINFGTMNGLGLDIDI
                     GTALITGIVFGIGVDYAIHLLSYIKHATREGKPLAQAIEAAIEHASWPIMVNSVCLAL
                     GFSVLVASHYGSTGRLGLLIAATMIVCALLTLIVLPVLVKLFKPKALQN"
     gene            complement(6896..>7720)
                     /locus_tag="VFX11_01630"
     CDS             complement(6896..>7720)
                     /locus_tag="VFX11_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4937344.1"
                     /translation="QGMQLASSLAPTNPMRRFLNRFILSELGPKLNTLIVGGATLDPS
                     TLTFFNNLGINAWQGYGLTETSPVAAVNSPGQIKRGSVGRAMPGCSIRIEAAPGEKIG
                     EILIKGPCIMNGYFNDRAYTREAIDQQDWFHTGDLGYVCDDGYLFITGRKKNLIVLDG
                     GKKVYPEEVEKALSASLLFQELCVTGLKVPHPSMPGKLTEQVSAVIVPQAQLRESKNL
                     ADLQTLCEQEVQRLCRELSSYKRPVQVIVQLDELPKTRTGKVKAPLVQQQLSASKA"
BASE COUNT         1448 a         2163 c         2502 g         1607 t
ORIGIN      
        1 cattctcgac gaacagaacc tggtccgcga actggagccg ttgcgcaacc tgccgtcgtc
       61 cacccaggac ggccaccaca tatcgctgat ggtgaacacc ggcctgctcg ccgatgtgca
      121 gcgctcgctg ggccgcggtg ccgagggcgt cggcctgtac cgcaccgaga ttccgttcat
      181 ggtgcgcgac cgctttccgt cggaactgga gcagatggaa atctaccgcc agcagctgga
      241 agcgtttcac cccgccagtg tcaccatgcg tacgctggac atcggtggcg acaaggcgtt
      301 gccgtatttt cccattcatg aagacaaccc gttcctgggc tggcgcggca ttcgcgtgac
      361 gctggatcat ccggaaattt tcctggtgca gatccgcgcc atgctgtggg ccagcgaaag
      421 cctggacaac ctgcgcatca tgctgccgat ggtgagttcc atcaccgaga tcgaggaggc
      481 gatgcacctg attcaccgcg cctatctgga agtgctggag gaaggcgccc atgtggtgat
      541 gccgcaggtg ggtgtgatga tcgaggtgcc tgccgccgtg taccagtgcc gcgagttcgt
      601 gcagcagtgc gatttcgtgt cggtgggcag caacgatctg acccaatata ttctggcggt
      661 ggatcgcaac aatcgacgag tggccaatct gtatcacgcg tttcatcctg ctgtgctgcg
      721 cgccttgcag acggtggtga ccgatgtgaa ggccgaaggc aagcccgtgt cgatctgtgg
      781 tgaactggcg ggcgatccgc ggggggcgat tctgctgatg gcgatgggtt atgacacgct
      841 gagcatgagt gccagcagtc tgctgcgggt gaaatgggtg ttgcgcaaga ttggtctggc
      901 acgtgccaag gaaattctgc agcaggtgtg gaccatggac aacgcccagg tgatccgcag
      961 ccatatggat ctgatgatgg agagggccgg tctgggcagc ttcatccggc ccatgcattt
     1021 tcccggtccg gcgaaagagc cgactgtcta gcaggccgca atgctgcggt tccggctggg
     1081 tgcgtgacgc gagcgcccag cgcgatattc ccgccgcagt ctttatttcc ccgctttgag
     1141 tttttccgct tccagcaccg acactttcac gccgatcacc agcacgccga tggcttcctt
     1201 gttatacagc atgggcacgg aaatctgtac ggcggtctgc ttggtgcttt cgtcttcttc
     1261 cacatcgccg atgaaaacct cacccttgcc gccggcaaag gccttggtga atttgtcctc
     1321 gtcgccctgc cagtaatcgc tggtggcggg gaaggctgcc acattggcgc ccttggcgtc
     1381 ggtgacgaac gcttcgttgt agacatccga ttgctgcacg atgatgtttt tcagaaatgc
     1441 gccgacttta ttttcctgta attcctggat cagcggtgat ttaccggcct tccattcctc
     1501 gtcgattttc tggatcgact ccaggctggc gttggcgcgg ttctgctcgc ggatcgcgtc
     1561 gagaatcatg gcgttcttgg tgagaaacat caggccgtcg atgcggggct tgagtatctg
     1621 ttgcagttcg gcctggctca tttcggcgtg gctggacagg ggttgcaggg ccagaacagc
     1681 gagaagggca aatgcggaca tcctgaacat gatgaaactc cttgataggt gcattttctt
     1741 gaagcttaga ccgcgttacg gcggttgtaa cgggtggctg gggtccgttt gtgtgtggct
     1801 ggcgcaagga gttgaattgg cgcaggtttt cgttacttgc ttaccggccg cttctgcagt
     1861 ttgcgctgca gggtgcggcg gtgcatgttg agggcgcggg cggtggcgga gatgttgccg
     1921 ttgtgttcca gcagcacccg ttgcaggtgt tcccattcca gccggtccac tgacagtgga
     1981 ttttcctgca ggggattggt gtcggtggtg ttgtcggcgt cgtcctgcag cagggccagg
     2041 atgtcggcgg cggtgaccgg tttgcacagg tagttttcag cgccgcgctt gatggcgtca
     2101 acggcggtgg cgacgctggc gtaggcggtg agcatcacca cccgggcgtc cggctggaga
     2161 gccagtatct gctgcagcag tttcaggccg ctttcgccgt ccaggttcag atccagcagg
     2221 atcaggtcga agcgcttgtg ctgcaggatt tgcagcgcgc tggtgccgtc ccgggcggtg
     2281 gccacttcgg tgccgcgccg ttgcagcgag cggcccaggg tctgcaggaa cagggaatcg
     2341 tcgtcgatca gcagcagggc cgggatggtg gtcatggagt ttccgttcct gccagtggca
     2401 ggcgcacttc ggtgacggtg ccgctggcgc gggtggatgg cagcaggcgc agggtgccac
     2461 ccagccggtt gatggtggcg tgggacaaaa acaggcccag ccccagaccg tcctggcgcg
     2521 aggtgacgaa ggcttccccc agttgcgggc gcacggcgtc gggaattccc ggtccggtgt
     2581 cggtgatgcg caggcagatg atattgccct gctgttcaaa ggccagctcg ggatcggtgg
     2641 cgctgacgcg ggcggcgttg tccagcaggt tgagcagggc ctggtccagg gtttcgtccc
     2701 cgaccacggt gccactgggc ggctgaccga tcacgcgtaa ttcccgcgcc ggatgcagca
     2761 ggttgaactg ctggcgcagg cgctgggtga gcagatcaat ggccaccggt tcgcgcacgt
     2821 cgttccgcaa attgtcggcc ttgtgcatca tgccgtgcag ggtggccttg caggtgtgaa
     2881 tctgttgcgc catcagttgc aggtcctgct gcaggtcggg ccggtccgtc agcgcgtctt
     2941 tcaggtcgtc ggtgagtacc gccagggtgg aaagcggcgt gcccaggtga tgggcggcgc
     3001 tggcggcgaa cgtggccatg gcaatcagct tttcgtcctg caggattttt tcctgctggc
     3061 gcgcgatctg gtcgcgctgg cggcgcatgg actgcgccag cggcagcacc agtcccacga
     3121 tcagcagcac cgacaccgca aaggtgatcc acattcccaa cagatgcagc tgcatgaaat
     3181 ggtcgctggc accggcgtgg ttgtggtgtg cgtggctggc cgtgtccccg gccatattgc
     3241 tgatcatctc aaggggaatg aaatattcgg tcagcgccgt gtacagcagc agcacgctga
     3301 gggcgatcag tgcggtggcc tgccatccca gcagggcggc gcccatggcc aggggcacca
     3361 gcagcaggga aatcaccgga ttggtgtggc cgccggcatc gtgcagatac cagcccagca
     3421 ggccaatgga gcagaacagc aacagcaggt agagcgccac tggcacggat gcgcgccggc
     3481 gccaggcgat aaaggtgacg gcggtgagca gggcctggga tggcagcagc agccagtgca
     3541 ggcgcgccgg caggctgatc tgttgcagat ggaaggcaag gctggccgtg agcatcaccg
     3601 ccagcaggat gcacatgtac caggcgaggt agcgcaggct gtcccgttgc aggggggcct
     3661 gccaccagga tgcggatgca tgggttgccg gttgcttcat ggggtggacc agtagggcgc
     3721 atcgatgcgg gtggaattgc ccgcattata cacgccggcc cgcgcgcagt tggttgtcgc
     3781 gcgggccggt gttgcttctt cagttttgca gcgccttggg cttgaacagt ttcaccagta
     3841 ccggcagcac gatcagcgtc agcagcgcac acacgatcat ggtggcggcg atcagcaggc
     3901 ccagacgacc ggtggagccg tagtgcgagg caaccagcac gctgaagccc agtgccagac
     3961 ataccgagtt caccatgatc ggccaggagg catgctcgat ggcggcttcg atggcctggg
     4021 ccaggggttt gccttcccgg gtggcgtgct tgatgtagga cagcaggtgg attgcatagt
     4081 ccacgccgat gccgaacacg atgccggtga tcagtgcggt gccgatatcg atatccagcc
     4141 ccagcccgtt catggtgccg aagttgatca ggatggtcag cgccagcgga atgacgctga
     4201 gcacgccggt ccaccaggag cggaacagca ggatcatcac gccggcaatc agcagggtcg
     4261 cgatggtgag gctgctgatc tgaccattca cgatttcgtc ggtgctggtc accatgatct
     4321 ggccgaagcc ggcaacctgc attttcacgt cggccggcaa tacggttctg gcgtatgcct
     4381 gcacatcctg cagcacagcg cgcaccttcg aagactggtc ggtgtgggac agcgccacca
     4441 gccgggcgtt cctgtaattc tcatccagcg agtcacgaat ttcctgccca ttgccgtttt
     4501 catacagcag gaaatattgc gcgatcagtt ccggtgacat atccgccggc agggcatagt
     4561 cggtattgtt gagcagctgg tgaatacgct tcaccagatt gctgggcgag ccaacgtaac
     4621 caatgatgtc atgctgctcg aaacgctgcg cgatctggtc catggcgatg atcacgtcag
     4681 gctgggtgaa acggcgttca tcggcagcgc tgaaccagac gttgatgggc acggtgccac
     4741 cgaagtgacg gttcaggatt gcatcgtcct gacggatacg ggtgtcctcg ctgaattgca
     4801 cgatgttgtg attgtccacg cgcacttcgc tcgacagata agtggtgaac gcggcgatgg
     4861 ctgcggtggc tgccagcacc agcagcgggg cacgacccag cgtcaggtac agtttgccga
     4921 aaccgcggtt gaccggattc atcagcgggc tttgcttgtt ctcgctggcg ttggccggct
     4981 gtttcagcag cggcagaatg gcgggcagca gggtgacgga aatgatgaag gcgaacaccg
     5041 cacctgcggc gatgaaaatg ccgaactcgc gcacgaacac caggctggtg tatgccagcg
     5101 cgatgaagcc gaagaaatcg gtgaggctgg tcatgaacat tggcttcatc agatgacgca
     5161 gcgccacttt cactgcttcc acccggtcgt gggtgttgag caggccataa tattccgtca
     5221 gataatgaat ggaatccgcc acggcaatgg tcatcaggaa caccggcagg ctggcggtga
     5281 cgatattcac atcgatgccg aacatggcca tgaatcccat ggtccagagt gtggacagcg
     5341 tgcacaccgt catggtcagc accactgcct gcaggcggcg gaaactgatg aaaagaatca
     5401 gacagattac tgtggacacc agcgggaaaa acagcatgtt gtcctgctgg atgatctgcg
     5461 cgatctgggc cgtgaccatc ggtgggccgg aaaaatgcaa ttgcgcatcg ccgccgtttt
     5521 cagccaacag cttttcaatg gcagcgtaca ccgcctgcat gttgggagag tcgtcttcgg
     5581 cgatattcag ttccacctgc atgttggtgg ccttgccatc ctgcgacacg atcacgccca
     5641 ccagcagcgg attggccatg atgtgctgtt gcagtgcctg cagtccggcg ctgtccgacg
     5701 gaatctgttc gatcagggaa tcgatcgcca gatcgtcgcc gtccacccgg atgtcttcca
     5761 cggtgaacag gctgcgcacg cgcttcacgg ggcgcgcccg cacgatgatg tcctcgattt
     5821 tctccagttc ctgggttgtc agggttttcg cagacttgcc gtgctgcacg agctggcgca
     5881 gcggctccac gtcttccagc ccgataccgt tttgcgcaac cgtggccacg atggcctgcg
     5941 cttcagtgtc atccccggcg atgctgcgca ggcgttccag gtcggtgtcg tcggcgaaag
     6001 agatccgttc cagctgctgg gtcagtttct gcaccagttc cagattggcg gtggtgaaaa
     6061 tgccgttctt ttcatccacc agcgccacga aagcctgttc ttcggtgttg gtgaaaatgc
     6121 ctttgatttc cgattcggtg acgcgccccg gatggtccgg cgaaatgaaa tacggcgtgg
     6181 cgttgatgcc gatgttgacg agaaacacgc tgaagaaaat cgtgaagccg gccagcagtc
     6241 ccagtaccag atgacgatac tgggtgaccc acaccagata ggtttccatg aaggctgcgg
     6301 cattgggttt gggaaagaag cggccggtgc gggtgatgaa atcacggtat tccgcgccga
     6361 agctggactg gctcagattg cgttcctcac gggcggcggt gtgattcagg atcagtatct
     6421 ggtaggtgag cagcagcaac tggaaccagt gcggtgcgat catcagcgcg gcggtgtggg
     6481 cgatgatgaa cgatgtgtaa aacggatgcc ggataaaccg gtaggcgccc caggtgacga
     6541 tagtgcgtgg cgcgtcattg tcctggtgcc acagggccag cggaatgcgg tgcgtgccca
     6601 tcgtcagcga gatcagcgcg atggaaccgc agctgagcag cacgcccagg gttttcatgc
     6661 tggcgtacat ttcgctgtcc agggacaggt tcggctccag gtagccccag tacactccga
     6721 acagggaaac cggccacaga aacagcggca acccggtgat gaaccacatc aggttgaacg
     6781 agccgtcctt gcggaagaaa aacgccgggg tggcgccgat atataaaaag ttaaaggtca
     6841 acaatatgac cgcagacaat tccacgcagt actccttccc tatgatgatt aaccgttatg
     6901 ctttcgaagc cgagagttgc tgttgcacca gcggcgcttt caccttgccg gtgcgggttt
     6961 tgggcagttc atccagttgc acgatgacct gcaccggccg cttgtaggac gacagttcgc
     7021 gacacaggcg ctgcacttcc tgttcgcaca acgtttgcag atccgccaga tttttcgatt
     7081 cccgcagctg ggcctggggc acgatcaccg cactcacctg ttcggtgagt ttgcccggca
     7141 tggacggatg cggcactttc aggcccgtca cgcacagttc ctggaatagc aggctggcgg
     7201 acagcgcttt ctccacctct tccggataca ccttcttgcc gccatcgagc acgatcagat
     7261 ttttcttgcg gccggtgatg aacaggtaac catcgtcgca gacatagccc agatcgccgg
     7321 tgtggaacca gtcctgctgg tcgattgctt cgcgggtgta ggcgcggtcg ttgaaatagc
     7381 cgttcatgat gcaggggcct ttaatcagga tctcgccgat tttttccccc ggcgccgcct
     7441 cgatgcggat gctgcatccc ggcatggcac gacccaccga accacgcttg atctgtcccg
     7501 gtgaattcac cgccgccacc ggactggttt cggtgagtcc gtaaccttgc caggcattga
     7561 tgcccaggtt gttgaaaaaa gtgagcgtgg acggatcgag cgtggcaccg cccacgatca
     7621 gcgtgttcag tttcggcccc agttcgctga ggatgaaccg gttcagaaaa cgccgcatcg
     7681 gattggttgg ggccaggctg gatgccagct gcatgccctg
//
LOCUS       DASUEG010000097         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1939902, whole genome shotgun sequence.
ACCESSION   DASUEG010000097 DASUEG010000000
VERSION     DASUEG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939902"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFX11_01635"
     CDS             complement(<1..1027)
                     /locus_tag="VFX11_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4937345.1"
                     /translation="MKKLTVIQTLPALEAGGVERGTLEIARALVAAGHRSIVISNGGR
                     MVEQLEREGSEHITMPVHRKSLASLFQIRPFRQKLAELKPDIVHARSRIPAWIAWLAW
                     RKLDPATRPHFVTTVHGLYSVSPYSAIMTKGERVIVISEAVQDYVRTNYPSCPPDRMQ
                     LIYRGADTAEFPYGLNPGPEWIADWEQQFPELKGKTVLALPGRISRVKGHETFLKLLK
                     ALEQRHPEVHGLIIGGAEAAKEDYAEEVRRKVRDMGLQDRVTFTGHRDDMNRVLTRCD
                     LVLALRTTPEAFGRTTLEPLRLGKPVIGWDVGGVGEMLRRIYPFGAVPAGREDLLLAR
                     VEEWLAHP"
     gene            complement(1039..2034)
                     /gene="lpxL"
                     /locus_tag="VFX11_01640"
     CDS             complement(1039..2034)
                     /gene="lpxL"
                     /locus_tag="VFX11_01640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEX4937346.1"
                     /translation="MNPPAFSKAFLAPKYWPAWLLIGLFWLMARLPWAVQRRLGAGLG
                     WLSWQLLGQRRDDTLVNLALCFPELDDDAREQMGRDTFRHAGIGIFETANAWFRSPEY
                     YRDRISFEGLEHLRAAEARGKGILLLGAHYSLLDLGAAMASLHFKVDTVYRPQPNPVF
                     EFVMTRRRLRYHHWTIPYRDMRLLIKALKAGHTVWYTPDQDFGRKHSVFAPFFGVSAA
                     TITTPPRLAQVNDATVLLIHFCRDKNEERYRFLITPPLDNYPTGDDITDAARINKELE
                     TLIRRSPAQYMWSHRRFKTQPVGQLPPYPPKRHAAERRQRRRAARAAARAAGKKS"
     gene            complement(2034..>2612)
                     /locus_tag="VFX11_01645"
     CDS             complement(2034..>2612)
                     /locus_tag="VFX11_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4937347.1"
                     /translation="ILAGGQGNQGLLEDLGLRQPAMERRTVHMVLIKHAGLPALHAHC
                     LGSSTKPRLTITSHPTRDGGRVWYVGGELAEEGAQRDDRLQVEHAQRELKELLPWIDL
                     RGARFKTLKLDRILPDGERGLFKPEHAFVEPRGNNIVAWPCKMTLAPNLGRQVLSLLE
                     KEGMKPEHPPLAEALPLLYPEIGQPAWETLFD"
BASE COUNT          465 a          783 c          855 g          509 t
ORIGIN      
        1 tcgggtgggc cagccactct tccacgcgcg ccagcagcag gtcctcacga ccagccggaa
       61 ccgcgccgaa agggtagatg cggcgcagca tttcccccac accgccgacg tcccagccga
      121 tgacgggttt gcccaggcgc agcggttcca gcgtcgtacg cccgaaggct tcgggggtcg
      181 tgcgcaatgc tagcaccaga tcgcaccggg tcagaacccg gttcatgtcg tcgcggtggc
      241 cggtaaacgt cacccggtcc tgtagcccca tgtcgcgcac tttccggcgc acttcctcgg
      301 catagtcttc ctttgcagct tcggcaccac cgatgatcag gccatgaacc tctggatgcc
      361 tttgctcaag ggccttgagt agcttgagaa aagtctcatg ccctttcacc cgtgagatgc
      421 gaccgggcag ggcgagaact gtcttgccct tgagttcggg gaactgctgc tcccaatcag
      481 cgatccattc tggtccgggg ttcaggccat agggaaattc ggcggtatcc gcgccacgat
      541 agatcagctg catgcggtcg ggcgggcagg atgggtaatt ggtgcgtacg tagtcctgca
      601 cggcctcgga gatcacgatg acgcgttcgc ccttggtcat gattgcgctg taggggctga
      661 ccgaatacag gccgtgcacg gtggtgacaa agtgcggccg ggtggccggg tccagcttgc
      721 gccaggctag ccaggcaatc cacgccggga tgcgggagcg ggcgtgcacg atgtccggct
      781 tcagctcggc aagcttctgg cggaacggcc ggatctggaa caggctggcc aaggacttgc
      841 gatgcaccgg cattgtgatg tgctcgctgc cttcccgctc cagttgctcg accatgcggc
      901 cgccgtttga gatgacaatg gagcggtggc cggcggccac cagggcgcgg gcgatttcca
      961 gtgtgccgcg ctcgacgcct ccggcctcga gggccggcag ggtctggatg acggtcagtt
     1021 tcttcatgat tgcttccgtc atgacttctt tccggcagcg cgtgcagctg cgcgcgcggc
     1081 acgacggcgt tgcctgcgct cggcggcatg ccgcttgggc ggataagggg gaagttgacc
     1141 gaccggctgc gtcttgaagc gccggtgcga ccacatgtat tgcgccggcg agcggcgaat
     1201 cagcgtttcc agttccttgt tgatgcgtgc ggcatccgtg atgtcatcac cggtggggta
     1261 gttgtccagc ggtggtgtga tcaggaagcg ataacgctct tcattcttgt cgcggcagaa
     1321 gtgaatgagc agaacagttg cgtcgttgac ctgcgccagt cggggcgggg tcgtgatggt
     1381 ggcggcactc acgccaaaaa acggtgcaaa cacgctgtgc ttgcgaccaa agtcctggtc
     1441 cggggtgtac cagaccgtat gtccggcttt gagcgccttg atcagcaggc gcatgtcgcg
     1501 atacggaatg gtccaatggt ggtaacgcag gcgacgacgc gtcatcacga attcgaacac
     1561 cgggtttggt tgtggacgat agaccgtatc caccttgaag tgcagggaag ccatggcagc
     1621 gcccaagtcc agcaggctgt agtgggcgcc cagcaacagg atgcctttgc cacgagcctc
     1681 ggcagcacgc aggtgttcca ggccctcgaa ggaaatgcgg tcacggtaat actcgggact
     1741 gcggaaccag gcattcgccg tttcaaagat gccgatgccg gcatggcgga aggtatcccg
     1801 gcccatctgt tcgcgcgcgt cgtcatcaag ttccggaaag cagagcgcca ggttgaccaa
     1861 cgtatcgtcg cgacgctggc ccaacagttg ccacgatagc cagcccagtc ctgcgcccag
     1921 tcggcgttgt acggcccatg gcaatcgggc catcagccag aacaggccga tgagcagcca
     1981 ggcgggccag tacttggggg caaggaaggc cttgctgaag gcgggaggat tcatcagtcg
     2041 aacagggttt cccaggcagg ttgcccgatt tccggataca gcaggggaag cgcttctgcc
     2101 agcggtgggt gctcgggctt catgccttcc ttctccagta gggacagcac ctgccgtccc
     2161 agattgggtg caagcgtcat tttgcatggc caggccacga tgttgttgcc tcgtggttcg
     2221 acaaacgcat gctccggctt gaacagaccg cgttcgccat ccggcaggat gcggtcaagc
     2281 ttcagggtct tgaagcgtgc acctcgcagg tcgatccagg gcagcagctc ctttagctcg
     2341 cgctgcgcat gctcgacctg caggcgatca tcgcgctggg cgccttcttc ggccagctcg
     2401 cctcctacat accatacgcg cccgccatcc cgtgtcggat ggctggtaat ggtcaggcga
     2461 ggcttggtgc tgctgccgag gcaatgggca tggagggctg gcaggccggc gtgcttgatc
     2521 aggaccatat gcactgtacg ccgctccatc gcgggttggc gcaggccaag atcctccaga
     2581 agtccctggt taccttgccc acccgccagg at
//
LOCUS       DASUEG010000098         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_790998, whole genome shotgun sequence.
ACCESSION   DASUEG010000098 DASUEG010000000
VERSION     DASUEG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790998"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFX11_01650"
     CDS             <1..305
                     /locus_tag="VFX11_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX4937348.1"
                     /translation="RDTLLPKVHIHTRFKPFVEDELPALAAGKRCLVAHPLPESQSCP
                     ATLNEQTLLAVGPEGGFIPYEVEKLQQAGFTAVTLGPRILKVETAIPALVARLFFP"
     gene            366..863
                     /gene="tpx"
                     /locus_tag="VFX11_01655"
     CDS             366..863
                     /gene="tpx"
                     /locus_tag="VFX11_01655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX4937349.1"
                     /translation="MAKITLKGNPVDTAGNLPAAGAPAPAFTLVKTDLSDVSLKDFAG
                     KKLVLNIFPSIDTPTCATSVRKFNQQASDMPNTQVLCVSADLPFALARFCGAEGIKNV
                     ISASVFRNPEFGTAYGAVITSGPLKGLLSRAVVVIDEQGKVLHAEQVGEIANEPNYEA
                     ALKVL"
     gene            complement(1012..1839)
                     /locus_tag="VFX11_01660"
     CDS             complement(1012..1839)
                     /locus_tag="VFX11_01660"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4937350.1"
                     /translation="MDWLFLCKALLLGILEGLTEFLPISSTGHLIVVGSWLEFDSGTG
                     KVFEVVIQLGAIMAVVWLYRMKIIHLLQGMLKGNVADLRFAGCVLLAFFPAVIIGVLA
                     IDFIKQVLFDPLVVAIALIVGGVILMLAERHAAEAKVTRIEDITWKRALGIGIAQCFA
                     MIPGTSRSGSTIVGGLLAGLSRQAATEFSFFLAMPTMLGAAVYDGYRHRDLLDQNDLW
                     LIAMGFTAAFVTALLVVRALVQFIASHSFRVFAWYRIGLGSLILVLLAFNFSFSANA"
     gene            complement(1839..2921)
                     /locus_tag="VFX11_01665"
     CDS             complement(1839..2921)
                     /locus_tag="VFX11_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX4937351.1"
                     /translation="MTLLYLAGGLVLLVLGAEFLVKGASRLAAAAGISPLVIGLTIVA
                     FGTSAPEMAVSVGAAWSGQADLALGNVVGSNIFNVLFILGVSALIVPLVVAQQLVRLD
                     VPIMIVASVATLLMGMDGAIGRIDGLILFAGILAYTVFLIRQSKKESAAVKDEYSQEF
                     GESQGKVGRYALNFVYIAGGLVLLVLGARWLVDSSVAIAQYFGVSELVIGLTIVAAGT
                     SLPEVATSVIASIRGERDIAVGNVVGSNIFNILSVLGLSSVVAPAGIEVAPAALVFDI
                     PVMIGVALACLPIFFTGYIITRWNGALFLFYYVAYTAYLIMAAIHHDALATFGATMLW
                     FVIPLTAITLLALAIRFHRNRQAVGI"
     gene            3145..3507
                     /locus_tag="VFX11_01670"
     CDS             3145..3507
                     /locus_tag="VFX11_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937352.1"
                     /translation="MQYKEIQEEADSLRRQARKSVSKFQTELDSMVGLVEAMRNHGEN
                     ALYGRIMLDHLDPIFKALENEDQAQAEREVAQYLSERTEFLTGMKPWVPSSTDIISNL
                     LNTVTATVLADLIEAFKG"
     gene            complement(3497..3793)
                     /locus_tag="VFX11_01675"
     CDS             complement(3497..3793)
                     /locus_tag="VFX11_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937353.1"
                     /translation="MDRRIMMLRLRNQLEQQVALAELADSVIVLQSLKSCYLEATRLV
                     EMAHPSVPDVEALALRLRAAALQPGPLSGQATLRIAASYHWLARQLESESLVSP"
     gene            4514..>5117
                     /locus_tag="VFX11_01680"
     CDS             4514..>5117
                     /locus_tag="VFX11_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4937354.1"
                     /translation="MLLKTKLFAPPIRQNAVPRLRLLQQLGQPAPARLTLIHAPAGYG
                     KTTLARQWLDQLPNPSAWLSLDLQDNDPVRFWRYVSGALGQPPRTAAGTPGDSDHEAW
                     IIDLLNRWNQPDHALPTLLILDDFHVIQEPVLLASVSWFLDRLPTSLHVLITSRSLPS
                     LHIPQRRVRNTVTEIGAGALSFGPAEARLFFEDTLQLSLTA"
BASE COUNT         1175 a         1672 c         1374 g          896 t
ORIGIN      
        1 cccgcgacac cctcctgccg aaggtgcata ttcacacccg cttcaaaccc ttcgtggaag
       61 acgaactgcc ggccctggcg gctggcaaac gctgcctggt ggcccacccg ctgccggaat
      121 cgcaatcctg cccagccacg ctcaacgagc agactctgct ggccgtcggc cccgaaggcg
      181 gcttcattcc ctatgaagtg gaaaaactgc agcaggcggg gttcaccgca gtaaccctgg
      241 gcccccgcat cctgaaagtg gaaaccgcca ttcccgcact ggtggcgcgg ttgtttttcc
      301 cctgaatcag cgccattctg acgcggcatt ttgtatcgcc attcttaatc aaggagcaga
      361 cacccatggc caagatcaca ttgaaaggta atccggtgga caccgccggc aacctgcccg
      421 ctgccggcgc gcccgccccc gccttcaccc tggtgaaaac cgatctgtca gacgtcagcc
      481 tgaaagattt cgccggcaag aagctggtgc tgaacatctt ccccagcatc gacaccccca
      541 cctgcgccac gtcggtacgc aagttcaacc agcaggccag cgacatgccg aacacccagg
      601 tgctgtgcgt gtccgccgac ctgccgtttg cactggcgcg tttctgcggt gctgagggca
      661 tcaagaacgt gatcagcgca tccgtcttcc gcaatccgga attcggcact gcgtatggtg
      721 ctgtgatcac cagcggcccg ctgaagggtc tgctgtcccg cgccgtggta gtgattgacg
      781 aacaaggcaa ggtgctgcac gccgagcagg tgggcgagat tgccaacgaa cccaactatg
      841 aagccgcgct gaaagtactg tgagctgacc cgggttgctg ccggcgatag aggaagcaac
      901 caccccaaaa aacggccttg ccatcccaat gacacggccg ttttttttat gctaccgcta
      961 tcgcgaaccc cgcctcagcc ccgttacgga attcaattct gccgcgcccg atcacgcatt
     1021 cgcgctgaac gagaaattga acgccagcaa caccaggatc agcgaaccca acccgatccg
     1081 gtaccaggcg aacacgcgga aactgtggct ggcaatgaac tgcaccagcg cccgcaccac
     1141 cagcagcgcc gtgacaaacg cggcggtaaa tcccatggcg atcagccaca ggtcattctg
     1201 gtccagcaga tcccggtgcc ggtaaccgtc atacaccgcc gcccccagca tggtgggcat
     1261 ggcgaggaaa aatgaaaact ccgtcgccgc ctggcgcgac aatcccgcca gcagaccgcc
     1321 cacgatggtg gagccggacc gcgatgttcc cggaatcatc gcaaaacact gcgcaatccc
     1381 gatacccagc gcccgcttcc aggtgatgtc ctcgatgcgg gtgacttttg cttccgccgc
     1441 gtgccgttcc gccagcatca gaatcacacc accgacgatc agcgcaatgg ccaccaccag
     1501 cgggtcgaac agcacctgct tgatgaaatc gatcgccagc acgccgatga tcacggcggg
     1561 aaaaaacgcc agcagcacgc agccggcaaa acgcaggtcc gccacattgc ctttcagcat
     1621 gccctgcaac aggtggatga tcttcatgcg gtatagccac accaccgcca tgatcgcccc
     1681 cagctggatc accacctcga acaccttgcc ggtgccggag tcgaattcca gccagctacc
     1741 caccacgatc agatgccccg tactggaaat gggcaggaac tccgtcaacc cttcgagaat
     1801 gcccaacaac aacgctttgc acaggaacaa ccaatccatc agatacctac tgcctgtcgg
     1861 ttacggtgaa aacgaatcgc gagcgccagc agcgtgatcg cggtaagggg aatgacaaac
     1921 cagagcatgg tggcgccgaa tgtcgccagt gcatcgtgat ggatggcggc catgatcagg
     1981 taagcggtgt acgccacgta gtagaacaga aacagcgcgc cattccagcg cgtaatgata
     2041 tacccggtaa agaaaatcgg cagacaggcc agcgccacgc cgatcatcac cggaatatca
     2101 aacaccagcg cggccggtgc aacctcgatg cccgccggcg ccactacgct ggacagaccc
     2161 agcaccgaca aaatgttgaa aatgttgctg cccacgacat tgcccacggc gatatcgcgt
     2221 tcgccacgga tactggcgat caccgacgtc gccacttccg gcagggaggt gccagcggcc
     2281 acgatggtca ggccgatcac cagttcgctc acgccgaagt actgggcaat ggcgaccgag
     2341 ctgtccacca gccagcgcgc acccagcacc agcaggacca gtccgcccgc tatatagacg
     2401 aagttcagtg catagcgacc gaccttgccc tgactttccc cgaattcctg gctgtactcg
     2461 tccttcactg cggcactttc cttcttgctc tgccggatca gaaacacggt ataggccagg
     2521 atgccggcaa acaggatgag tccatcgatg cggccgatgg caccgtccat acccatcagc
     2581 agagtggcaa ccgacgccac aatcatgatc ggcacatcca gccgcaccag ctgctgcgcc
     2641 accaccagcg gcacgatcaa tgccgaaacg ccgaggatga acaggacgtt gaaaatgttg
     2701 ctgcccacca cattgcccag cgccagatcc gcctggccgg accaggctgc acccacgctc
     2761 actgccattt ccggcgcact ggtaccaaac gccacgatgg tcaacccgat caccagcggc
     2821 gaaataccgg cagccgccgc cagccgtgac gcacctttca ccagaaactc agcgcccagc
     2881 accagcaaca ccaaaccacc ggcaagatag agcaatgtca tatgcagcca ctccctacag
     2941 gacaatcgaa aaacgcagac acccgcctgc gtaaagtgcc gggccaattt taaagcagca
     3001 ggctgccccg gcaacgccgg tggtccacta ttttttccgg acgggattac ggtcaggctg
     3061 acggggacca accacgccag gtgccaatcc gttcaactgg actatgcttt aaaaacgaat
     3121 ctgcaacacg cggagagaga cagcatgcaa tacaaggaaa ttcaggaaga ggccgacagc
     3181 ctgcgacggc aggcccgcaa gtcggtatcg aaattccaga ccgaactgga ttccatggtg
     3241 ggactggtgg aagccatgcg caatcacggc gagaacgccc tgtacgggcg catcatgctg
     3301 gatcacctgg accccatctt caaggcgctg gaaaacgaag atcaggccca ggcggaacgg
     3361 gaagtggcac agtacctgtc agagcgcacg gaattcctca cgggcatgaa accctgggta
     3421 cccagctcca ccgacatcat cagcaacctg ctgaacacgg tgaccgccac cgtgctggcg
     3481 gacctgatcg aggctttcaa gggctgacga ggctttcgct ttccagctgc cgcgccaacc
     3541 agtgataact ggcggcgatg cgcagggtgg cctgtccgga cagcggcccc ggctgcagtg
     3601 cagcggcccg cagccgcagc gccagcgctt ccacatcggg cacggacgga tgggccattt
     3661 ccaccagccg ggtggcttcc agataacagc tcttgaggga ttgaaggacg ataacggaat
     3721 cagccagttc cgccagagcg acctgctgtt ccaactggtt tcggagtctt aacatcatga
     3781 tgcggcgatc catgcgcgca gcttcctttc cttggatgta cctggatggt gttccggcaa
     3841 gccctgaagg ccgccgtcag gcacggggtt gattcctgac tgagaccggc atcaccggtg
     3901 cataactgat caaatcttaa cgccttattg cctgatctgg ctatcggcgc ttaccggtgc
     3961 tgacaaagat caaacagtcc ctacacaaac aaaaacccgc attttccatc gaggatcccg
     4021 gaaaatgcgg gtttatcgga attcgtcatt tttgttatgg ctgtcagtgg gttggatatc
     4081 agttgctgag tgcttccagc agcgaaccat ctggcttgga agagacatcc tctacctcga
     4141 ccgatgactg gtattcgctt gcactgactc cgcgacggtt caaatcctgt tcagatgtaa
     4201 ccgccacacc cgctatatta atagccccta ccagggccag tatcactatc aattgcataa
     4261 cttcaccttt tgctgacgac taataaaacg gctctgtagc aaggcgtacc aacaaggtcg
     4321 gttcagcaaa ggcttggtca atctggtccg ccaagcgcgg cggccaagat tttattgtgc
     4381 ccgcattata gtgccaaaaa cgccagactg cacttgataa ggcatcctga cggatgaccg
     4441 gcccgtctgc tttttgactt gtgacggggt ttcggaaaac actgatcggc cgtccagata
     4501 acaagaacag accatgctgc tgaaaaccaa gctgttcgcg ccgccaatcc ggcagaatgc
     4561 ggttccccgc ctgcgcctgc tgcaacagct ggggcaaccg gcgcccgccc gcctgaccct
     4621 gatccatgca cccgccggtt acggcaaaac cacgctggcg cggcagtggc tggaccagtt
     4681 gccgaatccc agtgcctggc tgtcactgga cctgcaggac aatgacccgg tgcgcttctg
     4741 gcgttatgtc agcggcgcgc tgggacagcc tccccgcacg gccgccggca cacccggcga
     4801 cagcgaccat gaagcctgga tcatcgatct gctcaatcgc tggaaccagc cggatcatgc
     4861 gctgccaacc ctgctgatcc tggacgattt tcacgtgatc caggagccgg tgctgctggc
     4921 cagcgtgagc tggttcctcg atcgcctgcc caccagcctg catgtgctga tcacctcgcg
     4981 cagcctgccg tcgctgcaca ttccccagcg ccgggtgcgc aataccgtga ccgaaatcgg
     5041 agcgggcgca ttatcgtttg gaccggcgga ggcacgcctg ttctttgagg acactttgca
     5101 actgtcgctg accgccc
//
LOCUS       DASUEG010000099         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_260509, whole genome shotgun sequence.
ACCESSION   DASUEG010000099 DASUEG010000000
VERSION     DASUEG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260509"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VFX11_01685"
     CDS             complement(<1..1413)
                     /locus_tag="VFX11_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX4937355.1"
                     /translation="MPTDVADKGLRFDIQAGLTAAAVVLPKSMAYATVAGLPVAVGLY
                     TAFLPMLVYALLGSSRVLSVSSTTTLAILAGTQLALVVPDGDPARLLVACATLTTLVG
                     IMLLLAAVLRLGFVANFISTPVLTGFKAGIGLVIVLDQVPKLLGIHIQKEGFFRDLLN
                     LVQEVPHTSLLTAGVAATTLALLIGMERWRPHSPAPLVAVAGGIAATWYFGLADMGLA
                     IVGTIPQGLPHLTLPDLSLIEQLVPGALGIALMSFTESIAAGRAFTDRDDPAIDANRE
                     LIATGAGNLAGAFFGAMPAGGGTSQTAVVRAAGARSQKASLVAAAMAAATMLLLAPLL
                     GRLPNAVLAAVVIVYSIGLIQPQEFIAIRKVRTMEFRWALIAFLGVLVFGTLQGILVA
                     IIVSLIGLASQTANPPVYVIGRKRDEDVLRPLSPEHPDDETFPGLLMLRPEGRIFFVN
                     APHVTETIRTLVAQHNPRILA"
     gene            complement(1413..1697)
                     /locus_tag="VFX11_01690"
     CDS             complement(1413..1697)
                     /locus_tag="VFX11_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937356.1"
                     /translation="MSDDQTDAGVITALIQRMTDFRLPRALDIKAKVDKGEVLDEFDI
                     KFLEEVFEDANAGRAYLVRHPELGDIVNKMVNLYKEITDKALANEQARQS"
     gene            complement(1779..2603)
                     /locus_tag="VFX11_01695"
     CDS             complement(1779..2603)
                     /locus_tag="VFX11_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937357.1"
                     /translation="MRVARFLLINSVLAATVTCATHAALAEDVIPDHPLLSDSLHVTL
                     SGFYSRNATTAALGPSGGGTGVSVDFEETLDLSDRDATPMLGVVWRATERWRMEVEYF
                     ELKRDATRRLSGDLEWGDETYTAGSDVDSTFNVSDTRISAGYSFFRTRDKELGAGVGL
                     HISKLEASLGTTNIATEGSDVTAPLPVINLYGAFALTDTWAIRLRTDWLSLTYGDYSG
                     DIRDMSIDVNYQPWRHMGLGFGVRNLVMDLDVDANDWHGQARVNFQGPTAFVSASF"
     gene            complement(2704..>2955)
                     /locus_tag="VFX11_01700"
     CDS             complement(2704..>2955)
                     /locus_tag="VFX11_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HEX4937358.1"
                     /translation="NRAVAVAMRDGPAAGLTLVDGLLERGELTDYHLAYATRADLCRR
                     LGRTEDARAAYQAALERVKLGAERRFLEQRLQALTNTAE"
BASE COUNT          558 a          898 c          967 g          532 t
ORIGIN      
        1 cgccaaaata cggggattgt gctgcgctac cagcgtgcgg atggtttctg tcacgtgggg
       61 tgcgttgacg aaaaagatcc ggccttccgg ccgcagcatc agcaagcccg gaaaggtttc
      121 gtcgtcggga tgttcaggcg acagcgggcg cagcacatct tcgtcgcgct tgcgaccgat
      181 cacgtagacc ggcggattgg cggtctggct ggccaggccg atcagcgaca cgatgatggc
      241 caccagaatg ccctgcaggg tgccgaacac cagcacgccc aggaaggcaa tcagcgccca
      301 gcggaattcc atggtgcgta ccttgcggat ggcgatgaat tcctgcggct ggatcaggcc
      361 gatcgagtac acgatcacca ctgctgccag cactgcattg ggcaagcggc ccagcagggg
      421 cgccagcaac aacatggtgg cagctgccat ggccgcagcc accagcgatg ccttctggct
      481 gcgggcaccg gcggcgcgca ccacggcggt ttgcgacgtg ccaccgccgg cgggcatggc
      541 accaaaaaat gccccggcca gattgccggc gccggtggcg atcagttccc ggttggcatc
      601 gatggcgggg tcgtcgcgat cggtaaaggc gcggcctgcc gcgatggatt cggtgaaact
      661 catcagcgcg ataccgaggg cgccgggcac cagctgttca atcagggaca gatccggcag
      721 tgtcaggtgt ggcaacccct ggggaatggt gccaacgatg gccagcccca tgtcggccag
      781 gccgaaatac caggtggccg cgatgccgcc ggccacggcc accagcggtg cgggggaatg
      841 gggccgccag cgttccatgc cgatcagcag ggccagagtg gtggcggcta cgcctgctgt
      901 cagcagcgag gtatgcggca cttcctgcac cagattcagc agatcgcgga aaaatccttc
      961 cttctgaata tggatgccca gcagcttggg cacctgatcg agcacgatca ccaggccaat
     1021 gccggccttg aatccggtca gtaccggggt ggaaatgaaa ttcgccacaa agcccagccg
     1081 cagcaccgct gccagcaaca gcatgatgcc caccagcgtc gtgagggtgg cacaggccac
     1141 cagcaagcgg gcggggtcgc cgtcgggtac caccagtgcc agctgggtgc ccgccagaat
     1201 ggcgagcgtc gtggtggaac tgacactgag tacccgcgag ctgcccagca gcgcataaac
     1261 cagcatgggc aggaaagcgg tataaaggcc caccgctacc ggcaggcccg ccaccgttgc
     1321 atacgccatg gattttggca gtaccaccgc ggcggcagta agacccgcct gaatatcgaa
     1381 acgcaggccc ttgtccgcca catcagtggg cattacgact ggcgcgcctg ttcgttggcc
     1441 agtgccttgt ccgtgatttc cttgtacagg ttcaccatct tgttcactat atcgcccagt
     1501 tcagggtggc ggaccagata ggcgcggccg gcgttggcat cctcgaacac ttcttccaga
     1561 aacttgatat cgaattcgtc cagcacttcg cctttgtcta ccttggcttt gatgtcgagg
     1621 gcccggggca ggcggaaatc ggtcatgcgc tggatcagcg cggtaatgac gccggcgtcg
     1681 gtttggtcgt cgctcatggt gtgctccttg agaggggtga agagtgtagg ggtaacaatt
     1741 ctctagccta atccaggtat gtcagcgatc gccagcgctc agaaggacgc gctgacaaaa
     1801 gccgtggggc cctggaaatt cacccgtgcc tgaccgtgcc agtcgttggc atcgacatcc
     1861 aggtccatca ccagattgcg cacgccgaag cccaggccca tatggcgcca gggctggtaa
     1921 ttcacgtcga tggacatgtc gcggatatcg ccgctgtagt cgccataggt gagcgacagc
     1981 cagtcggtgc gcagccggat ggcccaggtg tcagtcagcg caaaggcgcc gtacaggttg
     2041 atcaccggca gcggggcggt cacatcgctg ccttcggtgg cgatgttggt ggtgcccagg
     2101 ctggcctcca gtttggagat gtgcaggccg acgcccgcac ccagttcctt gtcgcgggtg
     2161 cggaagaagg agtagccggc gctgatgcgg gtgtcgctga cgttgaaggt ggaatccacg
     2221 tccgagccgg cggtgtaggt ttcgtctccc cactccagat ctccactcag gcggcgggtg
     2281 gcatcccgtt tcagttcgaa atattccact tccatgcgcc agcgctcggt ggcgcgccat
     2341 acaacaccga gcatgggagt ggcgtcgcga tcgctcaggt ccagggtttc ttcgaagtcg
     2401 acgctgactc cggtgccgcc accagagggc cccaatgccg cggtcgtggc gttgcgggaa
     2461 tagaagccgc tcagggtgac gtgcaggctg tcactcagca ggggatggtc gggaatgacg
     2521 tcttcggcca acgcggcatg ggtggcgcag gtaacggtgg cggccagtac gctgttgatc
     2581 agcaggaagc gggcgacgcg catgttgctt ttccttgtgc agatgactgg caggataatc
     2641 gcctcttttg gcgatgtgga tcatgaccaa acgcaaaacc caacacaaaa gtcgggcccg
     2701 cattcattct gctgtgtttg tcagggcctg caggcgctgt tccagaaacc gccgctccgc
     2761 gcccagtttt acccgttcga gcgctgcctg gtaggcggcg cgtgcgtctt cggtgcgacc
     2821 caggcgccgg cacaggtctg cccgggtggc ataagccagg tgatagtcgg tcagttcgcc
     2881 ccgttccagc aatccgtcca ccagcgtcag gcctgccgcc gggccgtcgc gcatggccac
     2941 cgccacggcg cggtt
//
LOCUS       DASUEG010000100         6178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1873237, whole genome shotgun sequence.
ACCESSION   DASUEG010000100 DASUEG010000000
VERSION     DASUEG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873237"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFX11_01705"
     CDS             <1..658
                     /locus_tag="VFX11_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX4937359.1"
                     /translation="IEGGWVSVDGKVIEAPQHQVRDDQKVTVHPDARPDPVLPVTILL
                     HKPAGIDADADTGPRPAFSLLNPDNRLAGDNPSIQTLHRHFTRLAAPLPLETEASGLL
                     VLTQDGRVIRKLNEDGDLLEQEYVADIEGSISDAALKNLQTPVTFDGEPLPPVKVSRQ
                     SDTRLRFAMKGALPGQVAWMCERAGLTLVSLKRLRIGRIALAGLPEGQWRYLHERERF
                     "
     gene            746..1555
                     /locus_tag="VFX11_01710"
     CDS             746..1555
                     /locus_tag="VFX11_01710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4937360.1"
                     /translation="MNRLSSWLVAGLFSVSALPLHAAVQTRVIDYKTADGTTLRGYYA
                     WDDAIREKRPGVVVVHEWWGLNDYAQRRARELAAMGYPALAVDMYGDGQTANNPKDAG
                     HLMHTVVVDTEKQFARSLAGLQLLKAQPEVDATRVAAIGYCFGGKMVLDMARRNMDLA
                     GVVSFHGALATATRAERGKIKAAVLVLNGEADGFIPQADIDALKMEMKAAGADFGFVN
                     YPGAKHGFTSPDADKLAKEHGMDIAYDPVADADSWERMQAFFNKIFGTRKN"
     gene            1559..2593
                     /locus_tag="VFX11_01715"
     CDS             1559..2593
                     /locus_tag="VFX11_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937361.1"
                     /translation="MTKKTMHPKAAALLDAHVAHITAQLSGKSLHTLIEDEIGQIMAD
                     AEQITLNEAVTPQMIKETARNYAVDLELSGAIPELVGEIARALYANPVHNLTTVGELL
                     PDGLFEEFLDKVLEMREARERFVHEVVANPVYSALASDILLEGIRGYVQQGRDIARQI
                     PGAARAARLGQSLLSQAFPMLEESLEDNLRKYVTRILEGILHRSEHFLLTHFHEDRLR
                     EISLEVWDVIKEKPVSEFKRSVSSLDMEEFFVIGYETWRELRNTAFYQAMIDTGIDCF
                     FEKYGETNLRELVDEMGITHEIMVSEAMRYAPHVVKMLKRKKMLEPMLRRNLEPFYSS
                     KAVLAILEAP"
     gene            complement(2597..3049)
                     /locus_tag="VFX11_01720"
     CDS             complement(2597..3049)
                     /locus_tag="VFX11_01720"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4937362.1"
                     /translation="MTVITLTADIAATPHQVYDYVTRPVRWKEWHPASQGVSDVDDES
                     LVAGRRFEEDVLSAGRRRHLTWLVEESRPGQRWRASAYMADGSTVRLTYEMVAGSNGT
                     RFTRTLEYTLRPLLLRWLNDLFLWKRVRAESEAALANLCARLSGQQAV"
     gene            3220..4110
                     /locus_tag="VFX11_01725"
     CDS             3220..4110
                     /locus_tag="VFX11_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937363.1"
                     /translation="MPSLIARAVKAGSRLTIRHQPKSPASLVRHLRLTMGNPPTLPLL
                     PRGVNQASFREGNLKGDWLRVAKPRQVILYLHGGGYVCGSPKTYHNLCGRLAKALDAD
                     VLMPAYRLAPEHPFPAAVDDAIEAYEFLLSQGWRGDQISVAGDSAGGGLTLALLLALR
                     DRQRPLPKCAVVYSPYADVTMTSPSRRYNDGKDDMLSYRMLEVGMDVYVQNGDHENPY
                     ASPVYGDYTGLPPLFITVCEQEIFRDDAYRVAEAARKAGVQVSLLARNDLLHVWPIFV
                     PFLPEAREDLEKVVRFIGSH"
     gene            complement(4169..4750)
                     /locus_tag="VFX11_01730"
     CDS             complement(4169..4750)
                     /locus_tag="VFX11_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937364.1"
                     /translation="MNDIFKNEHLVWDLGRLSDHDRATRFAMRFQQSLCVYSPPVQQL
                     YTNYEIIVPDDDNRKLIILPNPHAFHDTFNRINEDAIIQTSLFITPDDKGDLQLLIPM
                     SGGRQRAMPLAVGLNFIQNKLGPEVPFLPVLAKGDLREFKASRPMLHLHRIVLSKIDS
                     RISELEIRAIRKVIQDKLIKHFGFAAVGKASVA"
     gene            5007..6020
                     /locus_tag="VFX11_01735"
     CDS             5007..6020
                     /locus_tag="VFX11_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937365.1"
                     /translation="MHPTAATLLEAHTRHMLDRLSGPTLNTLLNEETAALCAWAATVP
                     VSKVTDEVRVRDFLLRNVFEVTPSETLLTQIGTLATVALKNPLNESTSLDQLLNAREY
                     DMIVDRIISLEDLRRQIVRAILQNPSVIQLISDLIYHGVKNYLVDENALAKKVPGMSS
                     LMKVGKGMMERMGSLDAALENTLKSYVKRNTRATMDLAERLVEHAMETPKLKAVSRQF
                     WQQIKSEKLHRVTQYITADDVDDVVRIGNTLWNHFRTTPYARDMLNDLVHAWFEQWGN
                     QSVMQVLLDIGLTPERLQVEVRQIGEPLVAELIASGHLEARIRLHLEVFYSSPEATAA
                     LGG"
BASE COUNT         1258 a         1981 c         1829 g         1110 t
ORIGIN      
        1 catcgagggc ggctgggtca gcgttgacgg caaagtgatt gaagcgccgc agcatcaggt
       61 gcgtgacgac cagaaagtca ccgtgcatcc cgacgcgcgt ccggatccgg tgctcccggt
      121 caccatcctg ctgcacaagc cggcgggtat tgatgctgat gccgataccg gcccccggcc
      181 ggccttctcc ctgctcaacc cggacaaccg cctcgccggc gacaacccct ccatccagac
      241 ccttcatcgc cacttcacac gactggccgc accgctgccc ctggaaacgg aggccagcgg
      301 tctgctcgta ctgacgcagg atggccgtgt gatccgcaag ttgaacgaag atggcgacct
      361 tctggaacag gagtacgtcg ccgatatcga gggctccatc agcgacgccg cgctgaagaa
      421 cctgcaaacc ccggttacct tcgacggaga accgttgcca cccgtcaagg tcagtcggca
      481 gagtgacaca cgactgcgct ttgccatgaa gggcgccctg cctggccagg tggcctggat
      541 gtgcgagcgt gccggcctga cattggtgtc actcaagcgg ctgcgcattg gccgcatcgc
      601 attggccgga ttgcccgagg gacagtggcg ctacctgcac gaacgcgagc gcttttgagt
      661 ctgcatctgc catgattgcc ggatgaccct gccgccttgc tgggctacca tcagggctct
      721 tttcatttgg acagggagag tactcatgaa ccgcctttcc tcctggttgg tggccggcct
      781 gttttccgtt tcggccttgc cgctgcacgc tgctgtgcaa acccgcgtga tcgactacaa
      841 gactgctgat ggcaccacgc tgcgtggcta ttacgcctgg gatgacgcca tccgcgagaa
      901 gcgccccggc gtcgtggtgg tacacgagtg gtggggcctc aacgactatg cccagcgtcg
      961 ggctcgtgaa ctcgccgcca tgggttatcc cgccctcgct gtcgacatgt acggtgacgg
     1021 ccaaaccgcc aacaatccca aggatgccgg tcacctgatg cataccgtgg tggtcgacac
     1081 cgagaagcag ttcgcccgct cactggccgg tctgcaactg ctcaaggcac aaccggaagt
     1141 ggacgccact cgcgtcgctg cgatcggata ctgctttggc ggcaagatgg tgctggacat
     1201 ggcgcgccgc aacatggatc tggccggcgt ggtgagcttc catggcgcac tggccactgc
     1261 cacccgcgcc gaacgtggca agatcaaggc ggccgtcctc gtgctgaatg gcgaggctga
     1321 cggcttcatc ccgcaggcag atattgacgc cttgaagatg gaaatgaagg ccgccggtgc
     1381 cgacttcggc tttgtgaact atcccggcgc caaacatggc ttcaccagcc ccgatgccga
     1441 caagctggcc aaagagcatg gcatggacat cgcctatgac ccggtagccg atgccgactc
     1501 atgggaacgc atgcaggcgt ttttcaacaa gattttcggc acgcgcaaga actgattcat
     1561 gaccaagaaa acgatgcacc ccaaggccgc tgcgctgctt gatgcccatg ttgcccacat
     1621 cacggcgcag ttgagtggca agtcactgca tacactgatc gaagacgaga tcggccagat
     1681 catggccgac gccgagcaga tcacgctgaa cgaggccgta acaccgcaga tgatcaagga
     1741 aacggcgcgc aattatgccg tggaccttga gctctcgggc gccatccccg aactggtcgg
     1801 cgaaatcgcc cgcgcgctct acgccaatcc ggtgcacaac ctcaccacag tcggcgaatt
     1861 gttgccggac gggctgttcg aggagttcct cgacaaggtg ctggaaatgc gtgaagcacg
     1921 cgaacgcttt gtgcacgaag tggtcgccaa tccggtgtac tcggcactgg cctcggacat
     1981 cctgctggaa ggcattcgcg gttatgttca gcagggccgc gacattgccc gccagattcc
     2041 cggtgccgca cgtgccgcac gtctcggcca gtccctgctg tcgcaggcgt ttcccatgct
     2101 ggaggagtcg ctggaagaca atttgcgcaa gtacgtgacg cgcattctcg aaggcatcct
     2161 gcaccgcagc gagcacttcc tgctcacgca cttccatgaa gaccggttgc gcgagatctc
     2221 gctggaagtg tgggacgtca tcaaggaaaa gcctgtcagc gaattcaagc gcagcgtcag
     2281 cagcctcgac atggaagagt tcttcgtgat cggctacgaa acctggcgcg agctgcgcaa
     2341 tacggccttc taccaggcga tgatcgatac cggcatcgac tgcttcttcg agaagtacgg
     2401 ggaaacgaat ctgcgtgaac tcgtggacga aatgggcatc acccacgaga tcatggtgag
     2461 cgaagcgatg cgctacgccc cccatgtggt aaagatgctg aaacggaaaa agatgctgga
     2521 gccgatgctg cgccgcaatc tggagccgtt ttactcctcg aaagccgttc tggcgattct
     2581 cgaagcgccc tgaacctcag acggcctgtt gcccgctcag gcgggcacac aggttggcca
     2641 gtgcggcttc ggactcggcc cgcacccgct tccagagaaa caggtcattc agccagcgca
     2701 acaacaacgg ccgtagcgtg tactccagtg tgcgcgtaaa ccgcgtgcca ttgctgccgg
     2761 ccaccatctc gtaggtcagg cgtacggtgc tgccatcggc catgtaggcc gacgcccgcc
     2821 agcgctggcc cggacggctc tcctccacca gccaggtcag gtgacgccga cgccctgccg
     2881 acagcacgtc ttcctcaaaa cgccgccctg ccaccagcga ctcgtcatcg acatcgctca
     2941 caccctgcga ggcgggatgc cactccttcc agcgcacggg gcgagtcaca tagtcataga
     3001 cttgatgcgg ggtagcggca atatcagcgg tcagggtgat aacggtcatc agggccggct
     3061 cggcaatcag ggagaccgct agcgtaatgc agcgaccacg ccgggaacag cggcaacctt
     3121 caggaattgg cccggatcga caatttcgcg acctgccaag ccatgacggc cccggggcaa
     3181 gcccgctagt atcgtggcgg tttccagagg agtccctgca tgcccagcct gattgcccgt
     3241 gccgtgaaag ccggctcccg cctgaccatc cggcaccagc ccaagtcgcc agccagcctg
     3301 gtacgccatc tgcgcctgac catgggcaat ccccccacgc ttcccctgct gcctcgcggt
     3361 gtgaaccagg cctccttccg tgaaggcaac ctcaagggcg actggttgcg ggtagccaag
     3421 ccccgtcagg tcattctcta cctgcacggt ggcggctatg tctgcggctc acccaagacc
     3481 taccacaacc tgtgcggccg cctggccaag gcactggatg ccgacgtact gatgccggcc
     3541 tatcgtctgg cccccgagca cccctttccg gcggcagtgg acgacgccat cgaggcctac
     3601 gaattcctgc tcagccaagg ctggcgcggc gaccagatct ccgtggctgg cgattcggcc
     3661 ggcggtgggc tcaccctggc cctgctgctg gccctgcgtg accgccagcg ccccctgccc
     3721 aagtgcgcgg tggtgtactc gccctatgcc gacgtcacca tgacctcacc cagccgccgc
     3781 tacaacgacg gcaaggacga catgctgtcc tatcgcatgc tggaggtagg catggatgtc
     3841 tatgtgcaga acggcgacca cgaaaatccc tacgcctcgc cggtctacgg cgactacaca
     3901 ggactgcctc ccctgttcat cacggtctgt gagcaggaaa tcttccgtga tgatgcctac
     3961 cgtgtggccg aagccgcccg caaggcgggc gttcaggtgt cattgctggc ccgcaacgac
     4021 ctgctgcatg tctggcccat cttcgtcccc ttcctgcccg aggcgcggga ggatctggaa
     4081 aaggtggtgc gcttcatcgg ctctcactga aaatgtcgcg cacaaaaaag cccgcatgac
     4141 gcgggctttt tcgtttccgg cagagggctc aggccacgct ggccttaccc acggcggcaa
     4201 agccgaagtg cttgatcagt ttgtcctgga tgaccttgcg gatggcgcgg atctccagct
     4261 cggaaatgcg gctgtcgatc ttgctcagca caatgcggtg caggtgcagc atgggacgcg
     4321 aggccttgaa ctcgcgcagg tcgcctttag ccagcaccgg caggaacggc acctccggcc
     4381 ccagcttgtt ctggatgaaa ttgagcccca cggccagtgg catggcacgc tgacggccac
     4441 cgctcatggg aatcagcaac tgcaggtcac ccttgtcatc gggcgtgatg aacaggctgg
     4501 tctggatgat cgcgtcttcg ttgatgcgat tgaacgtatc gtgaaaggca tgcggattgg
     4561 gcaggatgat cagcttgcgg ttgtcatcgt caggcacaat gatttcgtaa ttggtgtaga
     4621 gctgttgcac cggcggggaa tacacgcaca gcgactgctg gaaacgcatc gcaaaccgcg
     4681 tggcacggtc atggtcggag agccgcccca gatcccagac cagatgttca ttcttgaaga
     4741 tatcgttcat cggcaagccc aacgcataac aaccctaccg gcacgcacgg cgccggtaac
     4801 aagaaggata gacaaagact gagcatgtgc ctgcaactcg tcgcggacaa gcggctaccc
     4861 gtcaggcggc acgacagggc gatgactgcc tcccccgtcc ggcctgccgg atttgccggc
     4921 aggccgcgaa ccccctgcaa atcggcccat gccgacaagc agaattgcgg taaggttgcc
     4981 gcaaacagaa taacaaggag catccgatgc acccgacggc agccaccctg cttgaggccc
     5041 acacccgcca catgctggac cgcctgagcg gccctaccct gaacaccctg ctcaacgagg
     5101 aaaccgctgc cctctgcgcc tgggccgcca ccgtacccgt cagcaaggtt accgacgagg
     5161 tccgtgtccg tgacttcctg ctgcgtaatg tcttcgaggt cacgcccagc gaaaccctgc
     5221 tcacccagat cggcaccctc gccacggttg ccctgaagaa cccgctcaac gagagcacct
     5281 cgctggacca gctgctcaac gcccgcgaat acgacatgat cgtggaccgg atcatttcgc
     5341 tggaggatct gcgccgccag atcgtgcgtg ccattctgca gaatccgtcc gtcatccagc
     5401 tgatttccga cctgatctac cacggcgtga agaactatct ggtggatgaa aacgcgctgg
     5461 ccaagaaagt acccggcatg tcgtcgctca tgaaggtggg caagggcatg atggagcgca
     5521 tgggatcact ggatgccgcc ctggagaaca ccctcaagtc ctatgtgaag cgcaacacgc
     5581 gcgccaccat ggacctggcc gagcgtctgg tggaacacgc catggaaacg cccaagctga
     5641 aggccgtgtc tcgccagttc tggcagcaga tcaagagcga gaagctgcat cgcgtcaccc
     5701 agtacatcac cgccgacgat gtggatgacg tggtgcgcat cggcaacaca ctgtggaacc
     5761 acttccgtac cacgccatat gcccgcgaca tgctgaacga tctggtgcat gcctggttcg
     5821 aacaatgggg caaccagagc gtgatgcagg tgctgctgga tatcggcctt accccggaac
     5881 ggttgcaggt ggaggtgcgc cagatcggcg aaccgctggt ggccgagctg atagcgtccg
     5941 gacacctgga agcccgcatc cgtctgcatc tggaagtgtt ctattcctcg ccagaggcca
     6001 ctgcggcact gggcggctaa ctgtccggca aaggcatgca aaggggagct gcggctcccc
     6061 tttttgttgc ttcgtcgccg acagaaggca ctggcagtca cagatggaag gagtaccgtt
     6121 gggacaggca accaaaaagg atttggtcat gacaacaaca accgccgacg tcaccatc
//
LOCUS       DASUEG010000101         5522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_525752, whole genome shotgun sequence.
ACCESSION   DASUEG010000101 DASUEG010000000
VERSION     DASUEG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525752"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFX11_01740"
     CDS             <1..687
                     /locus_tag="VFX11_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX4937366.1"
                     /translation="DLGIALDGDGDRIGIVTPSGKIIWPDRLLMLCARDLLSRNPGAD
                     ILYDVKCTRDVAELVSSLGGRAIMCATGHSLMKAKMKETGAVVGGELSGHIFFNDRWY
                     GFDDALYTAARVLEILSMEPFGADDVFAEFPEKVNTPELHIKVSDASKFNIMKKLEDQ
                     GKFPGGNLVKIDGLRVDFPDSWGLVRASNTTPVLVARFEADTDAALEQVKTIFRDQLK
                     AVEPGLNIPF"
     gene            758..1660
                     /gene="argB"
                     /locus_tag="VFX11_01745"
     CDS             758..1660
                     /gene="argB"
                     /locus_tag="VFX11_01745"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4937367.1"
                     /translation="MTLSKDQALNIAQVLSEALPYIQRFTGKTIVIKYGGNAMETEEL
                     NNSFARDIVMMKLVGINPVVVHGGGPQIGDLLKKIGKESRFVDGMRVTDSETMDVVEM
                     VLGGLVNKDIVNLINNNGGRAVGLTGKDGQLIRAKKLVITRPSPELKEPEIIDIGHVG
                     EVEKINTGVVDMLVHSDFIPVIAPIGVGEDGASYNINADLVAGKVAEALKAEKLMLLT
                     NTAGLLDKAGKVLTGLSPAEVDALIADGTIYGGMLPKINCALSAVNNGVTSSHIIDGR
                     VPHAVLLEIFTDAGVGTLITRKSR"
     gene            1679..2278
                     /gene="slmA"
                     /locus_tag="VFX11_01750"
     CDS             1679..2278
                     /gene="slmA"
                     /locus_tag="VFX11_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX4937368.1"
                     /translation="MATSERKASRKEQILQALAHMLEATPGGRITTAALAKEVGVSEA
                     ALYRHFPSKAKMFEGLIEFVEQSLFSRISMILKEAPDAFTQCGQILQLLLGFSEKNPG
                     ITRILTGDAITGETDRLRQRVIQLFDRIETQLKQVLREAELKEGKRTRLSAAQSANLM
                     LSAAEGRIGQYVRSGFQRNPTSGWDDQWQLLGECLFRPA"
     gene            complement(2291..2920)
                     /locus_tag="VFX11_01755"
     CDS             complement(2291..2920)
                     /locus_tag="VFX11_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4937369.1"
                     /translation="MKVLLAMLLAVLVGTDAMAAVRMYRYENAQGVKVIDYQVPPEYV
                     SKGYEVLNSQGRVIEVVPRSLTPQELMAKMKDEKRKLDEARQAEEDKKLLTIFSSPAD
                     AERARDRKLEAIDAYINITKGNILKLKDDFNYTQAQAAERERAGQAVPDFQLEKIESL
                     QRQISQAEEAILEKEKEKVVIRAEYGKHIERLRELKQRRETSTPVPASP"
     gene            2996..3766
                     /locus_tag="VFX11_01760"
     CDS             2996..3766
                     /locus_tag="VFX11_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4937370.1"
                     /translation="MSSQLLLFAASLIANTLSALAGGGAGLLQLPVLLFLGLGFASAL
                     VTHKIASVALGIGASVRHFRESSLNRLFALFILACGLPGVVTGAMIILKFDDHFSKLA
                     LGGLTLGIGIYSILKKDLGQQMLLRNRDGRGYLIGGAALFAIGVLNGSLTSGTGLLVT
                     LWLVRWFGLDYKTAVAYTLVLVGLFWNGFGAITLAFQAPVQWDWLPALLVGSLVGGYL
                     GAHLSIIKGNQWVKRSFEVLTFVVGIKLIWDGITPFLV"
     gene            3954..4337
                     /locus_tag="VFX11_01765"
     CDS             3954..4337
                     /locus_tag="VFX11_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937371.1"
                     /translation="MNNSTSISILCVDDDLGFRRYLRQSLDQRFQIRYFALGFECLTR
                     LQDSHFDLILVDATLPDMPSDQLVDKLKAMPNLNLTPVVIVSYKDSEQERKRFARCQC
                     DGFLSKVASPAEITERLGKALHRAA"
     gene            complement(4410..>5522)
                     /gene="creD"
                     /locus_tag="VFX11_01770"
     CDS             complement(4410..>5522)
                     /gene="creD"
                     /locus_tag="VFX11_01770"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX4937372.1"
                     /translation="TQDALQGGILLPAEQERRHAVFLPAELQLQAVAEPEIRYRGLYQ
                     VPLYQAALELNARFDAPDLAALHLKPEQMLWQEARVVVHVSDIKALTGALNAVWNEQP
                     LDVLPGTTSEQSGDGFHFPVPLAAPEAPHQFHLRFQLHGSNQLFVTPAGKRSTLQMSG
                     QWPAPSFQGEWLPSTRTVDAGGFSAQWDIPFIAKGMPMQWEGESANLRLDGTRWVGVK
                     LQPEMDAYRKNQRSTKYQLLFLVMIFGFFWLFQWLTGIGVHPLQYLLTGASMCVFYLL
                     LLSLSEHLGFFTAYVLASAALVVQIGLYARSVLQSWKRAGGMALMLAGLLGYLGSLLQ
                     EQDFALLYGSLGVFAALSVVMYLTRRLQQVPPSQAA"
BASE COUNT         1097 a         1728 c         1625 g         1072 t
ORIGIN      
        1 gatctgggca tcgccctgga cggcgacggc gaccgcattg gcatcgtcac tccgtccggc
       61 aagatcatct ggcccgaccg cctgctgatg ctgtgcgccc gggacctgct gtcacgcaac
      121 cccggcgccg acatcctgta cgacgtgaaa tgcacccgtg acgtggcgga actggtgagc
      181 agcctggggg gccgtgccat catgtgcgcc accggccatt ccctgatgaa agccaaaatg
      241 aaggaaaccg gtgccgtggt gggcggcgag ctgtccggcc acatcttctt caacgaccgc
      301 tggtacgggt ttgacgacgc cctgtatacc gccgcccggg ttctggaaat cctgtccatg
      361 gaaccgtttg gcgccgacga cgtgttcgcc gagttcccgg aaaaggtcaa tacaccggag
      421 ctgcacatca aggtatccga cgccagcaag ttcaatatca tgaagaagct ggaggatcag
      481 ggtaaattcc cggggggaaa tctggtcaag atcgacggac tcagggtaga cttccccgac
      541 agctggggtc tggtgcgggc atcgaacacc accccggtgc tggtcgcccg ctttgaagcc
      601 gacacggatg ccgcgctgga acaggtgaaa accattttcc gcgatcagct gaaagccgtg
      661 gaacccggcc tgaacatccc gttttaaacc cgctcattcc ttgcagcaca gacgtaaccg
      721 ttaggacgtc ggcgcattgc cgccggcgag aaattccatg accctgagta aagaccaagc
      781 gcttaacatc gcccaggtgc tgtccgaagc cctgccctac atccagcgtt tcactggcaa
      841 gaccatcgtc atcaagtacg gcggcaatgc catggaaacc gaggaactga acaacagctt
      901 cgcccgtgac atcgtgatga tgaagctggt gggcatcaat ccggtggttg tgcatggcgg
      961 cggcccgcag atcggcgacc tgctgaaaaa gatcggcaag gaaagccgct tcgtcgatgg
     1021 catgcgcgtc accgacagcg aaaccatgga cgtggtggaa atggtgctgg gtggcctggt
     1081 caacaaggac atcgtcaatc tgatcaacaa taacggcggc cgcgctgtgg gcctcaccgg
     1141 caaggacggc cagctgatcc gggcgaaaaa gctcgtgatc acccgcccgt cgccggaact
     1201 gaaagaaccc gaaatcatcg acatcggcca cgtgggcgaa gtggagaaaa tcaacaccgg
     1261 cgtggtcgac atgctggtac acagtgattt catcccggtg atcgcgccga tcggtgtggg
     1321 cgaggacggt gcgtcctaca acatcaacgc cgatctggtg gcaggcaagg tggcggaagc
     1381 gctgaaagcg gaaaagctga tgctgctgac caacaccgcc ggcctactgg acaaggcagg
     1441 caaggtgctg accggtctgt caccggccga agtggacgcc ctgattgcgg atggcaccat
     1501 ttacggcggc atgctgccca aaatcaactg cgcactgagc gcggtcaata acggcgtcac
     1561 cagttcccat atcatcgacg gccgggtgcc gcacgcggta ttgctggaaa tcttcaccga
     1621 cgccggtgtg ggcaccctga tcacccgcaa gagccgataa cggcaagagc acgcaacgat
     1681 ggccaccagc gagcgcaagg catcccgcaa agaacagatc ctccaggctc tggcccacat
     1741 gctggaagcc acgccgggcg gacgcatcac cacggcggca ctggccaagg aagtgggcgt
     1801 gtcggaagcg gcgctctatc ggcattttcc cagcaaggcg aagatgttcg aggggctgat
     1861 tgaattcgtc gagcagtccc tgttttcccg catcagcatg atcctgaagg aagcgcccga
     1921 cgccttcacc cagtgcggcc agatcctgca gctgctgctg ggtttttccg agaaaaatcc
     1981 cggcatcacc cgcatcctca ccggcgacgc catcaccggt gaaactgatc gcctgcgcca
     2041 gcgggtgatc cagctgttcg atcgcatcga aacgcagttg aaacaggtgt tgcgggaagc
     2101 ggagctgaag gaaggcaagc gcacacggct gtcggcggcg cagagcgcca acctgatgct
     2161 gtcggcggcg gaagggcgga tcggtcagta tgtgcggtcg ggtttccagc gcaatcccac
     2221 cagcggctgg gacgaccagt ggcaattgct gggtgagtgt ttgttccggc cggcctgaca
     2281 gccgcaacgg tcagggtgat gccggcaccg gggtcgaggt ttcacgcctc tgcttcagtt
     2341 cccgcaatcg ctcgatgtgt ttgccatatt cggcgcggat cacgactttt tccttttcct
     2401 tttccagaat ggcttcctct gcctggctga tctgacgctg caggctttcg attttttcca
     2461 gctggaaatc cggtaccgcc tgaccagcac gttcacgctc cgccgcctgc gcctgggtgt
     2521 agttgaaatc gtccttcagc ttcaggatat tgcccttggt gatgttgatg taggcgtcga
     2581 tggcttccag tttgcgatcg cgggcgcgct cggcatccgc cgggctgctg aaaatagtga
     2641 gcagtttctt gtcttcctcg gcctggcgcg cctcatccag tttgcgcttt tcatccttca
     2701 tcttggccat cagttcctgc ggggtgaggg agcgcggcac cacttcaatg acgcgaccct
     2761 gggaattgag cacctcatag cctttgctca cgtactcagg gggcacctga taatcgatca
     2821 ccttcacgcc ctgggcattt tcatagcggt acatgcgtac cgccgccatc gcgtcggttc
     2881 cgaccagcac agccaacaac atggctaaca acactttcat tttgccgatt atcccctcat
     2941 agggctttgc tgtatcatcg cgccgctcgc gctcaatcca ggattgcctg tggacctgtc
     3001 atcccaactg ttgctgtttg ccgcctccct gatcgccaac accctgtctg ccctggccgg
     3061 tggcggcgcc gggttgctgc agcttcctgt gctgctgttt ctgggcctgg gttttgcgag
     3121 tgccctggtg acccacaaga tcgccagtgt ggccctcggc attggcgcca gtgtgcgcca
     3181 cttccgcgaa agcagcctca accgcttgtt tgcactcttc atattagcct gcgggctgcc
     3241 gggtgtcgtg accggggcca tgatcatact caaatttgac gatcacttct ccaagctggc
     3301 gctgggcggt ttgaccctgg gaatcggcat ttattccata ctgaagaagg atctgggcca
     3361 gcagatgctg ctgcgcaacc gggacggacg cggctatctg attggcggtg cagccctgtt
     3421 tgccatcggc gtgctcaatg gcagcctgac gtcgggcacc ggcctgctgg tgaccctgtg
     3481 gctggtgcgc tggttcggcc tggattacaa gaccgccgtg gcctacacgc tggtgctggt
     3541 gggcctgttc tggaacggtt ttggcgccat caccctggcc ttccaggcgc ccgtccaatg
     3601 ggactggctg ccggccctgc tggtagggtc gctggtgggg ggctatctcg gcgcgcacct
     3661 gtccatcatc aaaggcaatc aatgggttaa gcgcagcttt gaggtgctga ccttcgtggt
     3721 gggcatcaaa ctgatatggg acggaatcac accctttctg gtgtaaaacg ccaacatcct
     3781 caactggaac cgctctaaaa ccgaattttc tttttttttc cggcacccac ggaatttgcg
     3841 tcccggttcc ctgtggcttc cggccgcaac gtctatattt aaacggtcga cagtcaacaa
     3901 gcgcctgcct cgtggcgcac tcgcaatgac ggggaaaaac cggaggcata acaatgaaca
     3961 attcaacctc tatcagcatc ttgtgcgtgg acgacgacct gggcttccgc cgttatctgc
     4021 gccagtccct ggaccagcgc ttccagatcc gctatttcgc gctgggcttc gagtgtctga
     4081 cccgcctgca ggacagccat ttcgacctga ttctggtgga tgccaccctg cccgacatgc
     4141 cgtcggacca gctggtggac aagctcaagg ccatgcccaa tctgaacctg acgccggtgg
     4201 tcatcgtgtc gtacaaggat tcggagcagg agcgcaagcg ttttgcccgc tgccagtgcg
     4261 acggcttcct gagcaaggtg gccagcccgg cggaaatcac cgagcggctg ggaaaggcat
     4321 tgcaccgcgc cgcctgaggc cagtgcgctg atccagcacc gtttgcagcg gaccggcaat
     4381 ccggcccgct gcatcagagt ctgcactctt cacgctgcct gcgacggagg cacttgctgc
     4441 agccgccgcg tcagatacat caccaccgac agtgccgcga acacccccag cgatccgtac
     4501 agcagggcaa aatcctgctc ctgcaacagc gaccccagat accccagcaa tcccgccagc
     4561 atcagcgcca tgccgcccgc ccgcttccac gattgcagca cactgcgcgc atacagcccg
     4621 atctgcacca ccagcgccgc actcgccagc acataggcgg tgaagaaccc caggtgttcc
     4681 gacagcgaca gcagcaacag gtagaacacg cacatggacg caccggtaag caggtattgc
     4741 aacggatgca caccaatacc ggtcaaccac tggaacagcc agaaaaagcc gaaaatcatc
     4801 accaggaaca gcagctggta tttggtggaa cgctggttct tgcgataggc atccatttcc
     4861 ggctgcagtt tcacgccaac ccagcgggtg ccgtccagtc gcagattcgc gctctcgcct
     4921 tcccactgca tgggcatgcc cttggcaatg aacgggatgt cccactgcgc actgaaaccg
     4981 ccggcatcca ccgtgcgcgt ggacggtagc cattcgccct ggaaactggg cgccggccac
     5041 tgcccgctca tctgcaatgt tgaacgttta ccggcggggg tgacgaacag ctggttgctg
     5101 ccgtgcagct ggaaacgcag gtggaactgg tgtggcgctt cgggcgcagc cagcggcacc
     5161 ggaaaatgaa aaccgtcgcc ggactgttcc gacgtggtgc ccggcaacac atccagcggc
     5221 tgttcgttcc acaccgcatt cagtgcgccg gtgagcgcct tgatgtcgct gacatgcacc
     5281 accacgcgcg cctcctgcca cagcatctgt tcgggcttga gatgcagcgc tgccaggtcc
     5341 ggcgcatcga agcgggcatt cagttccagc gcggcctgat acagcggcac ctggtacagg
     5401 ccgcgatagc ggatctcggg ctctgccacc gcctgcaact gcagttccgc cggcagaaac
     5461 accgcgtggc gccgctcctg ctccgccggc agcaggatgc caccctgcag cgcgtcctgc
     5521 gt
//
LOCUS       DASUEG010000102         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_327018, whole genome shotgun sequence.
ACCESSION   DASUEG010000102 DASUEG010000000
VERSION     DASUEG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327018"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFX11_01775"
     CDS             complement(<1..559)
                     /locus_tag="VFX11_01775"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4937373.1"
                     /translation="MKHRHLDVLIIGAGLSGIGAACHLQKKCPNVRFAIIERRERIGG
                     TWDLFRYPGIRSDSDMFTLGYSFRPWTQTKMLADGPSIRTYIEDTARDYDVKRHIHFG
                     LKVISEEWDSKNCHWNVTVLNEKTSEIETFRAGFVFNCTGYYKYDAGYTPNIPGLDKF
                     KGKLIHPQHWPEDYDYSGKCVVVIGS"
     gene            complement(556..1524)
                     /locus_tag="VFX11_01780"
     CDS             complement(556..1524)
                     /locus_tag="VFX11_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937374.1"
                     /translation="MTSSFTPSTGSYGKKDSSTSSAKPTLFVGANGLVLRASQYGAFT
                     APIVLLLHGGGQTRHAWADTADTLARAGYCAITLDARGHGESDWCAQGDYSTQSLVAD
                     LHAVIHSLPMAPIIVGASMGGLTAMLALGENTSLHCSALVLVDVAPRLEQQGVRRIIE
                     FMRRHQDGFDSLEQVRDAVAAYNPHRPPSNDLTGLRKNLRRRDDGRLYWHWDPAFLDY
                     ARVPSETSESLFEKSSLERAARKLSMPVLLIRGFQSDVLSDQGARELLDLVPHAHYVV
                     LNQAGHMVAGDRNTIFTEAVLGFLQRAGKHTRSHHASSTFDHGVLT"
     gene            complement(1873..>3482)
                     /locus_tag="VFX11_01785"
     CDS             complement(1873..>3482)
                     /locus_tag="VFX11_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4937375.1"
                     /translation="PGDPVILNFNKPLNAASVFLDGAIEMRVDGTLVPKELAPMRHDG
                     ATVIIDGSNLQHNTDIEIVLTSLLRDTQGNPLSQDETLALRFEDLSLPDGSAANLRSP
                     VAAVFPGFSCALTDALLTGPRPDWRNGRCVGGQNSDPLLHVDSLYKDYPVRVTFNRSI
                     DPVTINDGTFFVERRDLDNNWVPVPGRLDILPQRVQFFPDTPWRIGDLYRYTLRSVPN
                     DADCGVNAICSPDGAPLQTRQISRSSASAPAPREGGPDMVNHFVAADEDDRYTLVGLR
                     VLPVVDVNANMVFDEEEQGVLVLPDNSIEAPPNHLRLELKDFSGVITQANLGCAIGES
                     CPEKRFAFLSSGALAAGPRTFDPNVQVNRRLIDGNPATDNFMSGAIRANVYPTTISTT
                     SVFLQARALAIITIGLDTGPLVLRLIPQGADEPFITGFISDTPDGPWFNATFDILVDA
                     PELKPALGPITLGHDIRSKRVRNVTLEGPLRFVDDGRLILKIRNPDPLLIPASINLAG
                     IGLASVQLEVPSLAADLTLTFLPVKDF"
BASE COUNT          771 a          935 c         1042 g          734 t
ORIGIN      
        1 cgctgccgat gaccactacg cacttgccgc tgtaatcata gtcttcgggc cagtgttgcg
       61 gatgaatcag cttgcccttg aatttgtcga ggccgggaat gttgggagtg taaccggcat
      121 catatttgta gtatccagtg caattgaata cgaagccggc acggaaggtt tctatctcgc
      181 ttgttttttc attaagaacc gtgacgttcc agtgacaatt tttactatcc cattcttccg
      241 aaatgacttt caatccgaaa tgaatgtgtc gtttgacgtc atagtcgcgc gcggtgtctt
      301 caatgtacgt tcgaatagac gggccgtcgg caagcatttt ggtttgcgtc cagggacgaa
      361 agctatagcc gagcgtgaac atgtcggagt ccgagcgaat cccagggtag cgaaacaggt
      421 cccaggttcc gccaattctt tcgcgccgct caataatggc gaaacgtacg ttcgggcatt
      481 tcttctgcag gtggcaggcg gcaccgattc cggacaggcc ggcgccgatg atcagtacat
      541 ccagatggcg atgtttcatg tcaatactcc atggtcgaaa gtcgatgagg catggtggct
      601 gcgagtgtgt tttccagccc tttgcagaaa gcccagcacg gcctcagtga agattgtgtt
      661 tctgtccccg gcgaccatat ggccggcctg attcagcacg acatagtgcg catggggaac
      721 caagtcgagc aactcgcgag ccccttgatc gctgagcacg tcgctctgga atccgcgaat
      781 cagtagcact ggcatggaaa gctttcgggc tgcacgctcc agactggatt tctcgaacag
      841 gctttcgctg gtttcagagg gaacgcgggc gtagtccaga aaggcaggat cccagtgcca
      901 gtaaagacgg ccgtcatccc gccggcgcag attcttgcgc aatcctgtca ggtcgttcga
      961 aggagggcgg tgcgggttgt aggcagcgac ggcatcgcgc acctgttcaa gactgtcgaa
     1021 gccgtcctga tggcgacgca tgaattcgat gatgcggcgc acgccctgtt gctccagtcg
     1081 tggcgccacg tcgaccaaca ccagtgcgga acagtgcagc gaggtattct caccaagtgc
     1141 caacatggct gttaggccgc ccatggaagc accgacgatg attggtgcca taggcagcga
     1201 gtgaatgacg gcgtgaaggt ctgcgaccag gctttgggtg ctgtaatcac cttgcgcaca
     1261 ccaatcgctt tcgccgtgcc cccgcgcatc cagagtgatg gcgcagtagc ccgctcgggc
     1321 aagagtatca gccgtatcgg cccaggcatg gcgtgtttgg cctccgccgt gaagcagcag
     1381 cacgatgggg gcagtgaacg cgccgtattg actggcccgg agcacaagac cattggcacc
     1441 gacaaaaagt gtcggtttcg ctgacgaagt cgacgaatcc tttttcccgt acgaaccggt
     1501 ggatggggtg aaactggatg tcatggccac ctccattctg tatgagcaca acaaattatg
     1561 attggtcagc caaccaaata atagtctaaa gaagtccagc tggcaaagcg tttctagcgg
     1621 ccgcccgtac aggcttctac tttttgtctt tttatccgga agtgcacctt aaatccagcg
     1681 tgtgaacgcc ggatacaaca gacacgagga ggccttcggg agctgggccg cgaaatgaaa
     1741 cagtcccttc tgaaaagtcg cctgacgccg tcacaaaaga aaatgccagt cagggattaa
     1801 ctgactggca ttaccccata acggggccaa atggcgtatg cccgggggca taacggcgag
     1861 tcggctttcg cttcagaagt ccttaacagg cagaaaggtt aacgtcagat cggcagcaag
     1921 ggagggaacc tccaattgca cactcgcaag accgataccc gccaagttga tactggccgg
     1981 aatcaggagt ggatcaggat tgcggatctt gaggatcaac cggccatcgt cgacaaagcg
     2041 cagtggcccc tcaagagtca cattacgcac ccgtttgctg cgaatatcgt gaccaagcgt
     2101 aatagggccc agtgccggtt tcaactcagg ggcgtccacc agtatgtcga aggtggcgtt
     2161 gaaccagggg ccgtccggcg tgtcggagat gaacccggtg atgaagggct catcagctcc
     2221 ttgaggtatt agcctgagca ccagtgggcc cgtgtccaga ccgattgtga tgatggccag
     2281 agcccgagcc tgcagaaaaa cgctggtggt gctgatggtg gttgggtaga cgttggcgcg
     2341 tatggctcca gacatgaaat tgtctgtggc cgggttgccg tcgatcaggc gccgattcac
     2401 ctggacattg ggatcaaagg tgcgcgggcc ggccgccaga gcgccgctgc tgaggaaggc
     2461 gaagcgcttt tccgggcaac tctctccgat tgcacagccg agattggcct gtgtgataac
     2521 gccgctgaag tccttcagtt ccagccgcag atgattgggt ggcgcttcga tgctgttgtc
     2581 cggaaggacc aggacacctt gctcttcttc atcaaacacc atattggcat tgacatccac
     2641 cactggtaga acgcgcaacc caactaatgt atagcggtcg tcctcgtcgg cagcgacaaa
     2701 gtgattcacc atatcaggac cgccctcgcg cggggcaggg gccgatgcac tgcttcgcga
     2761 gatttgccgg gtttgcagcg gtgcgccatc aggcgagcaa atggcattga cgccgcaatc
     2821 ggcatcgttc ggcaccgatc ttaatgtgta acgatacagg tcaccaatcc gccacggggt
     2881 atctgggaaa aactgaacgc gttggggcag gatatccagc cggccgggca cgggtaccca
     2941 attgttatcc aggtcgcggc gttcaacgaa gaaggtgccg tcgttgatcg tcaccggatc
     3001 tatgctacgg ttgaaagtca ctctgaccgg gtagtccttg tacagactat ccacatggag
     3061 cagggggtcg ctgttctggc cgcccacgca gcgaccgttg cgccaatcag gccgtggccc
     3121 ggtaagcagt gcgtctgtaa gcgcacagga aaagccggga aacacggcgg cgactggcga
     3181 gcgcagatta gcggcgctgc cgtcgggaag agacaggtct tcaaagcgaa gtgccagtgt
     3241 ctcatcctgg ctcaatggat tgccctgcgt atcgcgcagc aagctggtca ggacaatttc
     3301 gatatcagtg ttgtgctgca ggttactgcc gtcgatgatc accgttgcgc cgtcgtggcg
     3361 catgggcgcg agctccttgg gtaccagagt gccgtcgaca cgcatctcga tggcgccgtc
     3421 aaggaatacc gaagctgcat tcaggggttt attgaagttc agaataacag ggtccccggg
     3481 gc
//
LOCUS       DASUEG010000103         7244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1431441, whole genome shotgun sequence.
ACCESSION   DASUEG010000103 DASUEG010000000
VERSION     DASUEG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431441"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..907
                     /locus_tag="VFX11_01790"
     CDS             245..907
                     /locus_tag="VFX11_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX4937376.1"
                     /translation="MKAEIKGGPAFAYMDVELAPGDTITTESNAMSSMDASLELRSRF
                     NGGFFIALLRKFLGGETLFINDFSNPTEQPRRLTLVQPTPGDVRCIELNGNTLYLQPG
                     AFLACTPGVKIGVVFAGFISWLAREGLFRIAVSGNGKVWYGAFGALLEKEIDGEYIVD
                     TSHLVSYEPGIKLKLQLAGGIFSSLFGGEGLVTRVVGKGKIVIQTRSISGITGWINPR
                     LP"
     gene            918..1601
                     /locus_tag="VFX11_01795"
     CDS             918..1601
                     /locus_tag="VFX11_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX4937377.1"
                     /translation="MQIELINRPGNTAAKISLAEGETCTTESGAMIAMSGNMNITTST
                     HKKGSGGLLKALKRMVAGESLFLNHFEPEGGNGELWLGTTLAGDMTTIRLDNENLIVQ
                     GSSFLACEEGIDIDLGWQGFKSILSGESVFWVNLKGSGQVVLSSFGAIYPVDVDGDYI
                     VDTGHIVAFSETLDFSITKAGSSWLHSILGGEGLVCKFQGQGTVWCQSHNPLSFGQAL
                     SSGLRPRKV"
     gene            1642..2697
                     /locus_tag="VFX11_01800"
     CDS             1642..2697
                     /locus_tag="VFX11_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX4937378.1"
                     /translation="MNTEYSVKLTGQTQPHASIDDVVIAFARMAKLSEDQARNLFARA
                     PVVVKKGLDSATAEKFRSALEKIGAVATIEPPLGAAPSAPELSLAPLEPAPAAPAQKS
                     PDQSTSAQTPAAPRQPAAPVREVVVEEQPGYQFRIEGRPDYAFLTVQIPPDETLKVEA
                     SAMATMDTNLRMKTKLRGGLGRFVTGESIFVNEFTAEGGPAEIGIAPGAPGDLAHVFL
                     DGNVIFLQNSAFVAAAMDVTLETKWQGLVKGFFSGEKLFLIRASGHGDLWFNTYGALI
                     EIDVSGDYVVDTGNIVAFTEGLEYNVTKVGGYKSLFFSGEGFVCRFRGQGKVWIQTRG
                     VAAFTSWAHWFRPAAKG"
     gene            2804..3196
                     /locus_tag="VFX11_01805"
     CDS             2804..3196
                     /locus_tag="VFX11_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937379.1"
                     /translation="MRPLPLFSLTLLLLLPAGAALADDGEQYQETYQEMPVEESYPQE
                     SYPEETYPDQGYPEEQYPDQPIPDQTMPMEEPTHQEPEYSPEQAPEYQQQLMEFRRNC
                     EESATQAELPIEEREQFIQDCLQSQGLQ"
     gene            3332..3538
                     /locus_tag="VFX11_01810"
     CDS             3332..3538
                     /locus_tag="VFX11_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937380.1"
                     /translation="MSGASSDGNWDFNEFEEADSYEAIPNLESDNPKKAKKNKLDIRR
                     KLEDRLERKRLEDRIGKLDDDWFD"
     gene            complement(3569..4414)
                     /locus_tag="VFX11_01815"
     CDS             complement(3569..4414)
                     /locus_tag="VFX11_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4937381.1"
                     /translation="MDTDLLKTFLEVNTTRHFGRAADNLYLTPAAVSARVRQLEHILG
                     VELFIRNRNNIQLTPEGERLVPHAETMLLAWSHARQDVVMPADQGRRLNVGATYSFWR
                     FAMAEQVPRLYELLPDLSLRAEAHPVDILVRRLEERTLDLALLLEPPALAGFRADKIG
                     QIRLVLVSSDPEASVRTALRDGYVHVDWGDAFDLFFSKRFGDAAVPLLHTNLASIALD
                     YLLSHKAAAWLPQSVLESGEGKGLVQVKGVPAFSRPVHAVYRTGTERRDAIAAFVGRL
                     KPLKI"
     gene            4593..5198
                     /locus_tag="VFX11_01820"
     CDS             4593..5198
                     /locus_tag="VFX11_01820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX4937382.1"
                     /translation="MTFDVRPVLLENTWVRLEPLQPHHADALFAIGQHAGDWAYMPHG
                     CFTSIEQTRSWIADALALQEKGEQVSFVIIDRSNGAVAGSTRFLNIRRRDRGLEIGWT
                     WLGRDFQRSAVNTATKLLLLQHAFDTLGALRVELKTDARNLRSQAAIARLGATREGVF
                     RRHMIVQDGFVRDTVYFSITDQDWPRVRDGLLQKLGATHPA"
     gene            5212..6093
                     /locus_tag="VFX11_01825"
     CDS             5212..6093
                     /locus_tag="VFX11_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4937383.1"
                     /translation="MTANSALRNDLLLVLVTVLAAAGWMFSKNALNGIPPLYFLGIRF
                     LIAGATLCLIGAGQLRQLQRRNWLQALGMGSLFALALMIWILGLEHSEHVGEGAFISS
                     LGVVLVPLFARFVFREVLPLSTWVALPVAIAGLALLSLDKGLRLETGQILMLVSAALF
                     AFHFNLITRVVAQMPALALTAIQLCVVGAASCLAALPFETLPDPIPPDTWGWILAAAL
                     IASSARFFLQAYAQARVSASHAAVIMVLEPVWASLLAGYFLQESMSLSQLAGCALIFA
                     ALLVNRWRAIANLIKSG"
     gene            6101..6301
                     /locus_tag="VFX11_01830"
     CDS             6101..6301
                     /locus_tag="VFX11_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX4937384.1"
                     /translation="MEDRITELETRIAFQEHSLQELSDEMYRQQKQIERLDNICRMLL
                     QQMQDVSAALPAKPLDEKPPHY"
     gene            complement(6334..6888)
                     /locus_tag="VFX11_01835"
     CDS             complement(6334..6888)
                     /locus_tag="VFX11_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937385.1"
                     /translation="MSIRNALSTRYLRWLALAPSVLLLLSCASGGGTTKDAGGLAQSA
                     DPCGEVRKLIASHADGFASLRGARTRSRYADIFQANYHAVGSSCEIWQAGKGGTRYVC
                     TRAAPDEEVARAYHDKAVTNLRTCLDASWKTSTEARPADSGFQTRFERDGSPTVVLIQ
                     GVQAQNVLSPQWTVFYSIGDFRKP"
     gene            complement(6927..>7244)
                     /locus_tag="VFX11_01840"
     CDS             complement(6927..>7244)
                     /locus_tag="VFX11_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX4937386.1"
                     /translation="HAKTFAGDKRFVFVGSFNLDPRSVELNTEMGVVFESPELAGKMN
                     EVFEHDLLDHAYRLDLDEDGDLNWTTGENGRKIRFDQEPETGWWQRLSTRVMSVFVIE
                     SML"
BASE COUNT         1440 a         2358 c         2165 g         1281 t
ORIGIN      
        1 tcgctcatca gcacccaccc ggccaccgaa gaccgcatcg aaaacctgca ggaactgatc
       61 gcggcccaac cgcaccagcg tcatcgcgat ctggacgaac ctttcctaca cttgcaggat
      121 gccgtgaaaa actacgtgac ccactccggc gcggaacagg acgaaacgga gtgaacctcc
      181 gcattgccgc cagcgccggc aacccgcaca caccccgaat ggcttgaaaa aggagttatc
      241 cagcatgaaa gccgagatca agggcgggcc cgcctttgcc tacatggacg tggagctggc
      301 gcccggcgac accatcacca ccgaatccaa tgccatgtcc agcatggacg ccagcctgga
      361 actgcgctcg cgcttcaacg gcggattttt tatcgccctg ctgcgcaaat tcctcggtgg
      421 cgaaaccctc ttcatcaacg atttcagcaa tcccaccgag cagccccgtc gcctcaccct
      481 ggtgcaaccc acccccggcg acgtgcgctg catcgaactc aacggcaaca ccctctacct
      541 gcagccgggt gcgttcctgg cctgcacacc cggcgtcaaa atcggcgtgg tctttgccgg
      601 cttcatttcc tggctggcgc gggaaggctt gttccgcatc gcggtcagtg gcaatggcaa
      661 ggtctggtac ggcgcgttcg gcgcgctgct ggaaaaggaa atcgacggcg agtacatcgt
      721 cgacaccagc catctggtgt cctacgagcc gggcatcaaa ctgaagctgc aactggcggg
      781 cgggattttc tccagcctgt tcggcggcga gggcctggtc acccgcgtgg tgggcaaggg
      841 caaaatcgtg attcaaaccc gcagcatttc cggcatcacc ggctggatca acccgcgtct
      901 gccctgagga accggccatg caaatcgaac tgatcaaccg acccggcaac accgccgcga
      961 aaatcagcct ggcggaaggc gaaacctgca ccaccgaatc cggtgccatg atcgccatga
     1021 gcggcaacat gaacatcacc acctccaccc acaagaaagg cagcggcggc ctgctcaagg
     1081 cgctcaagcg catggtggcg ggggaatccc tgttcctcaa ccatttcgag cccgagggcg
     1141 gcaacggcga actctggctg ggcaccaccc tggccggcga catgaccacc atcaggctcg
     1201 acaatgaaaa cctgatcgtg cagggcagct cgttcctggc ctgcgaggaa ggtatcgaca
     1261 tcgacctggg ctggcagggt ttcaaatcca tcctctccgg cgagagcgtg ttctgggtga
     1321 acctgaaagg cagcggccag gtggtgctgt cgtcgttcgg cgccatctac ccggtggacg
     1381 tggacggcga ttacatcgtc gacaccggcc acatcgttgc cttcagcgag accttggatt
     1441 tcagcatcac caaggccggc tccagctggc tgcactcgat cctcggcggc gaaggtctgg
     1501 tgtgcaaatt ccagggccag ggcacggttt ggtgccagtc ccacaacccg ctcagctttg
     1561 gccaggccct cagcagcggc ctgcgcccac gcaaggtctg aaccggatcg atactgaacg
     1621 ggaccaacag gggaaaccga catgaatacg gaatacagcg tcaaactcac cggccagacc
     1681 cagccgcacg ccagcatcga cgacgtggtg atcgccttcg cccgcatggc gaaactgagc
     1741 gaggaccagg cccgcaacct gttcgcccgc gcgcccgtgg tggtgaagaa gggactagat
     1801 tcggccacgg cggagaaatt ccgcagtgca ctggaaaaaa tcggcgcggt ggccacgatc
     1861 gagccgccgc tgggagcagc accgtcagca ccggaactgt cactggcgcc gctcgaaccc
     1921 gcaccggctg cgccagcgca gaaatcgcct gaccagagca catccgctca gacgccggcg
     1981 gcaccccgcc agcctgccgc gcccgtgcgc gaagtcgtgg tggaagaaca gcccggctac
     2041 cagttccgca tcgaaggccg ccccgactac gccttcctca ccgtgcagat tccaccggat
     2101 gaaaccctga aagtggaagc ctccgccatg gccaccatgg acaccaacct gcgcatgaaa
     2161 accaagctgc ggggtggcct gggccggttc gtcaccggcg aatccatctt cgtcaacgaa
     2221 ttcaccgccg agggcggccc ggcggaaatc ggcatcgccc ctggcgcgcc cggcgatctg
     2281 gcccacgtgt tcctggacgg caacgtcatc ttcctgcaga actccgcctt cgtggccgcc
     2341 gccatggacg tcaccctgga aaccaagtgg cagggactgg tgaaaggctt cttctccggc
     2401 gagaaactgt tcctgatccg cgccagcggc cacggcgacc tctggttcaa cacctacggc
     2461 gcgctgatcg agatcgacgt cagcggcgac tacgtggtgg acaccggcaa catcgtcgcc
     2521 ttcaccgaag ggctggagta caacgtcacc aaggtgggcg gctacaaatc gctgttcttc
     2581 tcgggcgagg gctttgtctg ccgcttcagg ggccagggca aggtctggat acaaacccgg
     2641 ggcgtggcgg ccttcacctc ctgggcgcac tggttccggc ctgccgccaa gggctgagca
     2701 tccactccct gccgcccggg tccactattt ttgcgatgtt cgccgcgcct ggaagggggc
     2761 tgcggcgtta tagtgtcatc ctaccgttga tcaggaaggt gtcatgcgtc cactgcctct
     2821 tttcagcctc accctgctac tgctgctgcc ggccggcgcc gctctggccg acgatggcga
     2881 acagtaccag gagacctacc aggaaatgcc ggtggaggaa agttacccgc aggaaagcta
     2941 tccggaagag acttatccgg atcagggcta ccccgaagaa caatacccgg accagccgat
     3001 tccggaccag accatgccca tggaagagcc gacgcatcag gaacccgagt acagcccgga
     3061 gcaggcaccg gaataccagc aacagctgat ggagttccgc cgcaactgtg aggagtccgc
     3121 cacccaggca gaactgccga tagaggaacg ggagcagttc atccaggact gcctgcagtc
     3181 ccagggcctg caatgagcct gactgacaaa aaacgttcag cgtgacagca aaaatctttt
     3241 gctgtcattt gccaatctga ctgtttatta atgcgcgtgt tttcacagga gcatgtgatc
     3301 ctgtataccc tggagtggcg tgaggcaggc gatgagcggc gcaagtagtg acggcaattg
     3361 ggatttcaac gagtttgagg aagcggacag ctacgaggcc attcccaacc tcgagtccga
     3421 caaccccaag aaagccaaga agaacaagct ggatattcgc cgcaagctgg aagaccgtct
     3481 ggagcgcaag cgcctggaag accgcatcgg caagctggat gacgactggt tcgactgaac
     3541 cgtcacgcta tccgctattg gcctgctgct agatcttcag tggtttcagc cggcccacaa
     3601 aggccgcaat ggcatcccgg cgctcagtgc cggtccggta caccgcatgc acgggcctgc
     3661 tgaaggccgg cacccccttt acctgcacca atccctttcc ttccccgctc tccagcacgc
     3721 tctgcggcaa ccaggccgcc gccttgtggc ttagcagata gtccagggca atcgaggcca
     3781 ggttggtatg cagcagtggc accgccgcgt cgccaaaacg cttgctgaag aacagatcga
     3841 aggcgtcacc ccagtccaca tgaacatagc cgtcccgcaa tgcggtgcgg acgctggcct
     3901 ccggatcgct gctcaccagc accaggcgga tctggccgat cttgtcggca cggaagcccg
     3961 ccagggcagg gggctccagc aacagcgcca gatccagcgt gcgctcttcc agccggcgca
     4021 ccagaatgtc gacgggatgg gcctcggcgc gcaacgacag gtccggcagc agctcgtaga
     4081 ggcgtggcac ctgttccgcc atggcaaagc gccagaaact gtaggtggcg cccacgttga
     4141 gccggcgccc ctggtccgcc ggcatgacca catcctgccg cgcgtgggac caggccagca
     4201 gcatggtctc ggcatggggc accaggcgct ccccttccgg cgtcagctgg atgttgttgc
     4261 ggttgcggat gaacagctcc acccccagga tatgctccag ctgccgtacc cgggcactga
     4321 cggcagcggg cgtcaggtac aggttgtccg ccgcccgacc gaaatggcgg gtggtgttca
     4381 cctcaaggaa cgttttcagc aaatcggtat ccatcggcgg cgctcgtcgt acctgtggca
     4441 cccggccgtt gagacctggt ggtgccagca gttgatgcgg aaaggccggg caagcttagg
     4501 ggaacaaaag cggcctgcgc aagggtccac cctggtctat tgtggtaacg taacggccct
     4561 cccccgagga cacccgcact aggaccgacc gcatgacatt cgatgttcgc cccgttctgc
     4621 ttgaaaacac ctgggtacga ctggagccgc tgcaaccgca ccacgccgac gcgctgttcg
     4681 ccatcggcca gcacgccgga gactgggcct acatgcccca cggctgtttc acctcgatcg
     4741 agcagacccg cagctggatt gcggacgcgt tggcgctgca ggaaaaaggc gagcaggtca
     4801 gcttcgtcat catcgaccgc agcaacggtg ccgtggccgg cagcacccgt ttcctgaaca
     4861 tccgtcgccg cgatcgcggc ctggaaatcg gctggacctg gctgggccgg gattttcagc
     4921 gcagcgccgt caacaccgcc accaagctgc tgctgttgca gcatgcattc gacacgctgg
     4981 gcgcactgcg ggtagaactg aaaaccgatg cccgcaacct gcgctcacaa gcggcgattg
     5041 cgcgcctggg cgccacccgc gaaggcgtgt tccgccgcca catgatcgtg caggacggct
     5101 tcgtgcgcga caccgtgtat ttcagcatca ccgatcagga ctggccccgg gtgcgcgatg
     5161 gcctgctgca gaaactgggc gccacccatc cggcatgacg acccgctgcg catgaccgcc
     5221 aactctgccc tcaggaatga tctgctgctg gtgctggtga ccgtgctggc cgccgccggc
     5281 tggatgtttt ccaagaacgc gctgaacggg attccgccgc tgtattttct cggcatccgt
     5341 ttcctgattg ccggcgccac cctgtgcctg atcggggccg gccagctgcg gcaactgcag
     5401 cgccggaact ggctgcaggc ccttggcatg ggcagcctgt tcgcgctggc attgatgatc
     5461 tggattctgg ggctggaaca cagcgaacac gtgggggaag gtgcgttcat ttccagcctg
     5521 ggcgtggtgc tggtgccgct gttcgcgcgt ttcgtttttc gtgaagtgct gccgctgtcc
     5581 acctgggtgg cactgccggt ggcgattgcg ggcctggcgc tgctgtcgct ggacaagggc
     5641 ctgcggctgg aaaccgggca gatcctgatg ctggtgtccg ccgcactgtt cgcgtttcat
     5701 ttcaatctga tcacgcgcgt tgtagcgcag atgccggccc tggcgctgac agcgatccaa
     5761 ttgtgtgtgg tgggtgctgc gtcctgcctg gcggcactgc catttgaaac cctgccggac
     5821 cccattccgc ctgacacctg gggctggatt ctggcggcgg cgctgatcgc cagcagcgcg
     5881 cgttttttcc tgcaggccta tgcccaggcc cgcgtctctg ccagccatgc cgccgtgatc
     5941 atggtactgg agccggtgtg ggcttcattg ctggcgggct attttctgca ggaatccatg
     6001 tcgctgtcgc agctggccgg ctgcgcactg atttttgccg cgctgctggt gaaccgctgg
     6061 cgcgccatcg ccaatctgat caaatccgga tgacctcaca atggaagacc gcatcaccga
     6121 actggaaacc cgcattgcgt ttcaggaaca cagcctgcag gaactgagcg acgagatgta
     6181 ccgccagcag aagcagatcg agcggctgga caacatctgc cggatgttgc tgcaacagat
     6241 gcaggatgtg tccgccgccc tgcccgccaa acccctggac gaaaagccgc cccattactg
     6301 agcagctcgg cctgtcccgg ggccctggcc ggtttacggt ttgcgaaaat caccgatgga
     6361 ataaaatacg gtccactgcg gactgagcac gttctgcgcc tgcacaccct ggatcagcac
     6421 caccgtgggc gaaccatcac gctcgaagcg ggtctggaaa ccactgtcgg ccggacgcgc
     6481 ctccgtgctg gttttccagg atgcgtccag acaggtacgc agattcgtca ccgccttgtc
     6541 gtgataggcg cgtgccactt cctcatccgg cgcagcgcgg gtacaaacat aacgggtgcc
     6601 ccccttgccc gcctgccaga tttcgcatga cgagccgaca gcatggtagt tcgcctgaaa
     6661 aatgtcggca taacgggaac gggtgcgggc accccgcagt gatgcaaacc cgtccgcatg
     6721 gctcgcaatc agcttgcgga cttcaccgca gggatcggcg ctctgggcca gcccgccggc
     6781 gtccttcgtg gtgcctccgc cgctggcgca ggacagcagc aacaacaccg acggtgccag
     6841 tgccagccat cgcagatatc gggtcgacag tgcgtttcgg atcgacatga agtgaattct
     6901 ccctggtttt aaaatgtgcg cgtcaatcaa agcatgcttt cgatgacaaa tactgacatc
     6961 acgcgggtgg acaggcgctg ccaccagccg gtttccggtt cctgatcgaa gcgaatcttg
     7021 cggccgtttt cgccggttgt ccaattcagg tcaccatcct cgtccagatc cagccgatag
     7081 gcgtggtcca gcaggtcatg ctcgaatacc tcattcatct tacccgccag ttcggggctt
     7141 tcgaacacca cacccatttc ggtattgagc tcgacggagc ggggatccag attgaacgaa
     7201 cccacgaaca caaaccgctt gtcaccggca aaggtcttgg catg
//
LOCUS       DASUEG010000104         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1409521, whole genome shotgun sequence.
ACCESSION   DASUEG010000104 DASUEG010000000
VERSION     DASUEG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409521"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFX11_01845"
     CDS             complement(<1..1362)
                     /locus_tag="VFX11_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4937387.1"
                     /translation="MLRVLLSLCLVWFGVGAALADTAMAPLLLQIGGSADRVPLNHAV
                     RYLEDPQGRLTVSDLQGRDAGWQAGGSGAFNQGYSSSVWWLHLQLRNDGDKVLERYLE
                     LSYAVLDYVDVHVYAGADALKTFQLGDLYPYHERPVDSRFFVLPLQWQPEQTLDVYFR
                     IKTSSAVQAPLTLWRQDAFVRSENNSNIAQGLYYGAMVVIAVYNLLIFLVLLERSYLF
                     YVGFVMSLPMFMAAVSGQGFRYLWPDAVAWNDHAIPFFLASAFMFAALFARRFLQVGK
                     WSPLANKMLLVSAACAGTCGVLAFVMPYYISIHLLVPLGLFVIILDMVVGVLALFHQV
                     PNARYYLIAWSAFLVGALMFALNKLGVLPANFLTEYSMQIGSMLEAVLLSFAMAERIN
                     VERKLRFEAQTEALLVTRRLNEELEQRVQERTLALEQANRKLEELSTTDQLTGLKNRR
                     YLDT"
     gene            1571..2017
                     /locus_tag="VFX11_01850"
     CDS             1571..2017
                     /locus_tag="VFX11_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006645769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX4937388.1"
                     /translation="MKILVDADACPNPVKEILFRAADKRAVETLLVANQRVNVPPSPH
                     IRAIQVPKGFDVADNEIVLRAEPGDLIITADIPLASAVIDKGAFALNPRGEFYTTENI
                     RQRLNMRDFMETLRSSGIQSGGPPPLTQQDRQAFANALDRFLTKNS"
BASE COUNT          541 a          758 c          613 g          384 t
ORIGIN      
        1 tgtgtccaga taacgccggt tcttcaggcc ggtaagctgg tcggtcgtgc tcagctcctc
       61 cagcttgcgg ttggcctgtt ccagcgccag ggtccgctcc tgtacccgct gctccagctc
      121 ctcgttcagg cgacgggtta ccagcagcgc ttcggtctgg gcttcgaaac gcagcttgcg
      181 ttccacgttg atgcgctccg ccatggcgaa ggacaacaga acggcttcca gcatcgaacc
      241 gatctgcatg gaatattcgg tgagaaaatt ggccggcagc acgccgagct tgttcagcgc
      301 aaacatcagc gcacccacca gaaaggcact ccaggcgatc aggtagtagc gtgcattcgg
      361 cacctggtgg aacagcgcca gcacacccac caccatgtcc agaatgatga caaacagtcc
      421 caatggcacc agcaggtgga tcgagatgta atacggcatc acgaaagcca gcacaccgca
      481 ggtaccggca caggcagccg ataccagcag cattttgttc gccagcgggg accatttgcc
      541 cacctgcagg aaacggcggg caaacagggc ggcaaacatg aacgcgctgg caaggaagaa
      601 cggaatcgcg tgatcattcc aggccacggc atcgggccac agatagcgaa aaccctgccc
      661 gctcaccgcc gccatgaaca tcggcagcga catcacaaat cccacgtaga acaggtagct
      721 gcgctccagc agcaccagga agatcagcag gttgtacacc gcaatcacca ccatggcacc
      781 gtaatacagg ccctgggcga tattgctgtt gttttccgag cggacaaacg catcctgccg
      841 ccacagggtc agtggcgcct gcacagcact gctggtcttg atgcggaagt acacatccag
      901 cgtctgttcc ggttgccact gcaacggcag cacgaaaaag cgactgtcca caggacgctc
      961 gtgataggga tacagatcgc ccagctggaa ggtctttaac gcgtcggccc cggcgtacac
     1021 atgcacgtcg acataatcca gcaccgcata ggacagttcc agatagcgtt ccagcacctt
     1081 gtcgccatca ttgcgcaact gcagatgcag ccaccagacg gaactgctgt agccctggtt
     1141 gaaggcgcca ctgccgccgg cctgccagcc ggcatcccgc ccctgcaggt cggacaccgt
     1201 caagcggccc tggggatctt ccagatagcg caccgcatgg ttcaggggga cccggtcggc
     1261 agacccgcca atctgcagca gcaacggcgc catcgccgta tccgccaggg cagcgcctac
     1321 accaaaccag accagacaaa gggacaaaag aacgcgcagc atgcaggcgg acctcggctt
     1381 caggagaaag agaacgggtt tacaaccggt cgcatccagc gctgactgat aagttccatt
     1441 actcaagaat agccgactcg ctggaacaat cttgcctttt tgtgccagga ggggcaccgc
     1501 acccggcttg ccagcggtac cgtgatccgc catgctgccc tgattcgaac cccgtaccgg
     1561 agccaacccc atgaaaatcc tggtggatgc cgacgcctgc cccaatccgg tcaaggagat
     1621 cctgttccgg gcagcagaca agcgcgccgt ggaaaccctg ctggtggcca accagcgcgt
     1681 caacgtgcca ccctcccccc acatccgcgc catccaggtg ccaaagggct tcgacgtggc
     1741 cgacaacgaa atcgtactgc gggccgagcc gggcgatctg atcatcaccg ccgacattcc
     1801 cctggcttcc gctgttatcg acaagggcgc cttcgccctc aacccccggg gcgagttcta
     1861 caccactgaa aacatccgcc agcgcctgaa catgcgggat ttcatggaaa ccctgcgctc
     1921 cagtggcatc cagtccggtg gaccgccacc gctcacccag caggaccgcc aggccttcgc
     1981 caatgcactg gatcgctttc tgaccaaaaa cagctgaaac tgatcaaggc atgagcaccc
     2041 ataaaacagt tcagtctata ccctgcacct gcatatagat tttctatcct gaaaatacag
     2101 acaatcaatt ttatcaatca tcgaaatttg cctagtcttg ctcctgtagc gggccggaag
     2161 ctgtcactgt ccggcccggc aatcgcccaa actgaatgga gagcagacca tgagcaacac
     2221 gcccttgacc acctccgccg gcgcaccggt ggccgatgac aacaacagca tcagcgtcgg
     2281 accgcgcgga ccgctc
//
LOCUS       DASUEG010000105         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_591758, whole genome shotgun sequence.
ACCESSION   DASUEG010000105 DASUEG010000000
VERSION     DASUEG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591758"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2176)
                     /locus_tag="VFX11_01855"
                     /pseudo
     CDS             complement(71..2176)
                     /locus_tag="VFX11_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
     gene            complement(2288..2437)
                     /locus_tag="VFX11_01860"
                     /pseudo
     CDS             complement(2288..2437)
                     /locus_tag="VFX11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
     gene            complement(2497..>2673)
                     /locus_tag="VFX11_01865"
     CDS             complement(2497..>2673)
                     /locus_tag="VFX11_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX4937389.1"
                     /translation="KITIKHGDIAHLDMPGMTMVFTARNKDMLQNVKAGDKIRLMVVY
                     EGGKMVVTDIQPVR"
BASE COUNT          549 a          788 c          738 g          598 t
ORIGIN      
        1 cctatacctt ctggcgccgt taccagtttg gcagggggga ttttggtccc gggatctgaa
       61 gcaccggatg ctaactgagc tttcgacctt taagacgcaa agcattccct attacagatg
      121 cagagctgaa gctcatcgcc aatgccgcaa tcatgggcga tagcagccat cctgtcagtg
      181 ggtacagcac gccagccgca atgggaatac ccaatgcatt gtagagaaat gcaaacgcca
      241 ggttctgctt catattgtcc acagtggcct cagacaacgt gcgcgccaca gcaatgcccc
      301 gtaaatcacc ttttatcagt gtgacctgcg cgctattcat ggccacgtcg gtgcccgtgc
      361 ccatggccac tccaacgtca gccttcgcca gagcaggcgc atcgttaatg ccgtcgcccg
      421 ccatggcaac gatgcgtcct tctcgctgca gtttttcgac cagctgtaat ttatccgcag
      481 gcttgacctc accatgcacc tcatcgatac ccaggcgcgc accgaccgct tttgctgtgg
      541 tcaggccgtc ccctgttgcc ataacgatac gcaaacctgc tgctttcagt gcggacaacg
      601 cttccggtgt gctggccttg atcggatctg ataccgcgag caagcccgcc aattttccat
      661 ccacggcgag gtgcatcacg ctggctccct ctgctcgcag gttttcagcc tgtggcagca
      721 atacatcaat cgatacattc tgttgcagca tcaacgtggt atttcccagt acaagggtgc
      781 gccctgcgac aatgccctgt acgccgatac cggaacctga ttcgaaatgg tccggttttt
      841 ccagggccaa accccgttca cgcgccgcct gaacgatcgc atcagccagg ggatgttcgc
      901 tgccctggtc caggcttgcg gccagacgca agacctccga ttcggtgaag cccggcgcag
      961 caaccgcacg gtcaaaggca gggcgtcctt ccgtcagtgt gccggttttg tcgatgataa
     1021 gtgtgtcgat cttgcgcagg tgttcgatgg cggccgcatc gcggaagagc acaccttgcg
     1081 tggctccccg tccggtagcg accatgatgg acatgggtgt ggccagtccc agtgcgcagg
     1141 ggcaggcaat gatcagcacg gccaccgcat tgattaaccc gaatacccag cgcggctcgg
     1201 gtccgaacaa gccccaggaa aaaaatgtca gcacggcaat gcaaaccacc gccatgacga
     1261 aatagcccgc caccacatcg gccatgcgct gcatgggtgc cttggaacgt tgcgcctgcg
     1321 ccaccatctg cacgatttgc gacagcatgg tttccgatcc cacgcgttcg gatcgcatca
     1381 cgattgcgcc attgatgttc agtgtcgcac caatgacctt gtcgcccacc cgtttggtca
     1441 cgggcactgg ctcgcccgtc agcatggatt catctaccgc gctgctgcca tcggttaccg
     1501 taccatccac tggaactttt tctccaggcc ggacccgcaa ccggtcaccg acatggacat
     1561 gggcgagcgg tacatcctcc tcagtgccat cgtccagaat gcggcgggcg gttttgggtg
     1621 ccaaacccag cagtgacttg atggcggcgg atgtttgcga gcgggccttg agttcaagca
     1681 gctggcctag caaggtcaac gaaatgatga ccgctgccgc ctcgaagtac accgcgaccc
     1741 gtcccatgga gacgaaagaa tccgggaaca cctgtggcgt cagggttgct accaggctgt
     1801 agatgaatgc agcgcctgta cccagactga tcagcgtcca catgttaggg ctgcggtggg
     1861 cgacagactg ccagccgcgc agaaaaaatg gccatccggc ccagaggaca atgggcaacg
     1921 tcagaacgag ttcaatccag gtctgggtgc gggcatcgaa ccagtgcagc tggtgcccga
     1981 acattgcgag tactgtgact gcgatggtca gcggaagagt ccaccagaac cggtgctgaa
     2041 aatcgcgcag ttccggattg ccgtcctcct cgagctctgg cagcaagggt tccagagtca
     2101 tgccgcactt tgggcaattg ccgggatgat cttgccggat ctcgggatgc atgggacagg
     2161 tataaatcgt actgccacca ctttccggcg ctgttgccga ctggacttct gacgtcgcga
     2221 tggcagtgtg atgatggtgt ccggttttcg cggtatgcgc tgtgtgatcg tgactggtat
     2281 tcatggatta tccccgtgtc tcatcgccag gtttactaag caagctgggt atgaacctgg
     2341 gagtcttttg agcgtaggtt tcatacattg caccgaactt tttgtgcatt tcgttttctt
     2401 cggtaaccgc caggcgtcca tacattacga gcgatacgtt gcatgtctct gggcacagca
     2461 tgacagctgt gattgatcgg tgggaatgtc attttcctac cgaacaggct ggatgtccgt
     2521 gacaaccatc ttgccgcctt cataaactac catgagcctg attttgtctc cggctttgac
     2581 gttctggagc atatccttgt tcctggcagt gaataccatg gtcatgcccg gcatgtcgag
     2641 gtgcgcaata tcgccatgtt tgatcgtgat ctt
//
LOCUS       DASUEG010000106         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1607890, whole genome shotgun sequence.
ACCESSION   DASUEG010000106 DASUEG010000000
VERSION     DASUEG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607890"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX11_01870"
     CDS             complement(<1..385)
                     /locus_tag="VFX11_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase TmcA
                     domain-containing protein"
                     /protein_id="HEX4937390.1"
                     /translation="MTASAHRLELESLLARCGARRWRLPVLVQGSHEQCLRWCVDNLP
                     VRDVLWVSRSAPSGGWQLPPGKANHELGREADCVVVDLFDGLNADTLAAVAGMVRAGG
                     CLVLLGPPLEEWGDFPDPEYDRIRVE"
     gene            439..1161
                     /locus_tag="VFX11_01875"
     CDS             439..1161
                     /locus_tag="VFX11_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937391.1"
                     /translation="MDKKYSKRVRYEFIFKSGEEFQYQIDMDPDTLEHPVPVGMPPEW
                     AKLDVDKCSICPLKSSISEYCPLALRIAPILSFAPHPAFESVQIRVHKDNMTIESYTT
                     VQDAYRSLIGLIMATSGCPHTGFFKPMAWFHLPFATQDETLFRACATFLFFQFFDPAD
                     PEQGSHFSDLKQVYENIHQVNQQIAKRLRRASASESTLNALTILDIFAQSFLPTLNQS
                     LQQLAFLFKPHINELNFEMPKD"
     gene            1166..>3080
                     /locus_tag="VFX11_01880"
     CDS             1166..>3080
                     /locus_tag="VFX11_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937392.1"
                     /translation="MKRRLSSLPLVKKLVIAFLVGGLVPMLILGLVSLNNANDTIRTQ
                     VSNQLLAVRDQKSHEIARYFDVIRKQATVLASEPAIVAAALQLPDAFRDYARETELDP
                     QEIAKLREELAHYYKQQFGTEFARLNNGIKPDIMTPLQQLDDASVVMQHSYISANPNP
                     LGSKHLLDAAAIDTRYNRMHARLHELLRPYVEKFGYYDAFIADASSGLLVYSVFKELD
                     FATSLKNGPYADSNLGAAFRKGLTLQQNASVMVDFDHYRPSYDAPASFVAAPIVSNGK
                     TIALLILQIPINHINAIMTDRSGMGETGESYLVGPNQLMRSDSFLSPQFHTVIASFRN
                     PEKGKVDTEATRRALAGESGVDTIIDYNGNPVVSAYSTVDLGDFRWAVLAEQDVAEAF
                     APERSLLLSTLLTAGLCTAALIGLGLYLSRLIAHPINQVAAVLKTVSTRGDFTVRAKT
                     SSTDEVGQMAQAFNDFLQTLGESFRQINEVLTAVSDGNTRVRVNDAYMGDIAALAQGV
                     NSAIAKINDFQDQQKLAAAKLQQSALALEEKVNESAEMARLANVEAERANRIKQALDV
                     ASTSVMMTDAQNNITYTNAALDSMMREHEADIRKALPQFRANALMGANMDQFHRNPQH
                     QRNIIGNLHGTHEA"
BASE COUNT          711 a          974 c          808 g          587 t
ORIGIN      
        1 gctccacccg gattcggtca tactcgggat cagggaaatc accccattct tcaagaggag
       61 gccccagcag caccaggcat ccccctgccc gcaccatgcc ggccacggcc gccagggtgt
      121 ccgcattgag gccatcgaac agatccacca ccacgcagtc ggcttcccgt cccagctcat
      181 gattggcctt gcccggcggc aactgccagc cccccgacgg cgccgaccgc gacacccaca
      241 acacatcccg taccggcagg ttgtcgacac accagcgcag gcactgctca tggctgccct
      301 gcaccagcac cggcagccgc cagcgtcggg cgccacaacg ggccagcagg gattccagtt
      361 ccaggcgatg ggcagatgcg gtcatatggc ggaaattacc tatactgaag aaaggcttgg
      421 atcaggaagc atcttctcat ggataaaaaa tacagcaaac gcgtccgcta tgaattcatt
      481 ttcaaatccg gcgaggaatt ccagtaccag atcgacatgg accctgacac gctggaacac
      541 ccggtgccgg tgggcatgcc gccggagtgg gcaaagctgg acgtggacaa gtgcagcatc
      601 tgcccgctga aatccagcat ctccgaatac tgcccgctgg ccttgcgcat cgcgccgatc
      661 ctcagcttcg cgccgcaccc ggcatttgag tcggtgcaga tccgcgtgca caaggacaac
      721 atgaccatcg aaagctacac caccgtgcag gatgcctacc gttcgctgat cggcctgatc
      781 atggccacca gcggctgccc gcacaccggc tttttcaaac cgatggcctg gttccacctg
      841 cccttcgcca cccaggacga aaccctgttc cgcgcctgcg ccaccttcct gttcttccag
      901 tttttcgatc cggcagaccc ggagcaaggc agccattttt cggatctgaa acaggtttat
      961 gaaaacattc accaggtgaa ccagcagatc gcgaagcgat tgcggcgggc gtcggcgtcg
     1021 gaatccacgc tgaacgcgct gacgattctg gatatcttcg cccagtcgtt cctgcccacc
     1081 ctcaaccagt cgctgcaaca gctggcgttc ctgttcaagc cgcatatcaa cgagctgaat
     1141 ttcgaaatgc ccaaggattg atagtatgaa gcgccggctg tcttcgctcc cgcttgtcaa
     1201 aaaactggtt attgcatttc tggtcggcgg actggtgccg atgctgatcc tcgggctggt
     1261 ttcgttaaac aacgccaacg acactatccg tacccaggtt tccaaccagt tactggccgt
     1321 gcgcgaccag aaatcccacg aaatcgcacg ctactttgat gtgatccgca agcaggcaac
     1381 cgtcctagcg agcgaacctg ccattgtcgc cgccgccctg cagttacccg atgcatttcg
     1441 tgattacgcc cgtgaaaccg agttggaccc acaggaaatt gcgaagctgc gggaagaact
     1501 ggcccattat tacaagcagc aattcggtac cgaatttgcg cggttaaaca atggcatcaa
     1561 acccgacata atgactccgc tgcagcagct ggacgacgca tccgtcgtga tgcagcacag
     1621 ctacatttcg gccaatccca acccgctggg atcgaaacac ttgctggatg cagccgctat
     1681 cgacacccgg tacaaccgga tgcatgcgcg tctgcatgaa ctgttgcgcc cctatgtgga
     1741 aaagttcggc tattacgatg ccttcatcgc tgacgccagc agcggactgc tggtctattc
     1801 cgtgttcaag gaactggatt tcgccacatc gttgaaaaat gggccctacg cggacagcaa
     1861 cctgggcgca gccttccgca aagggttgac gctgcagcag aatgcctcgg tcatggtgga
     1921 tttcgaccac taccgtccat cctacgacgc gcccgccagc tttgtcgcag cccccattgt
     1981 cagcaacgga aaaaccattg cgctgttgat actgcaaatc cccatcaacc acatcaacgc
     2041 catcatgacc gaccgcagcg gcatgggaga aaccggtgaa tcctatctgg tgggacccaa
     2101 ccagctgatg cgctcggatt cgtttctgtc gcctcagttt cacacggtca ttgcatcgtt
     2161 tcgcaatcct gaaaagggca aggtggacac cgaagccacc cgccgtgcgc tggctggcga
     2221 gtctggagtg gataccatca tagactacaa cggcaacccg gtcgtatctg cgtattcgac
     2281 ggttgatctg ggtgatttcc gctgggccgt actggccgaa caggatgtgg ctgaagcatt
     2341 cgctccggag cggtcactgc tgttatccac cctgctgaca gccggattgt gcaccgcagc
     2401 actgatcggc ctggggctgt atttaagccg actgattgcc caccccatca accaggtagc
     2461 tgcggtattg aaaactgtca gcactcgcgg cgatttcacc gttcgggcca aaaccagcag
     2521 taccgacgaa gtgggacaga tggcgcaggc ttttaacgat tttctgcaaa ccctgggcga
     2581 atcatttcgc cagatcaacg aggttctgac agccgtcagc gatggcaaca cccgcgtcag
     2641 agtgaacgac gcgtacatgg gggacattgc cgcactggcg cagggcgtga acagcgcaat
     2701 cgccaagatc aatgatttcc aggaccagca aaagctggcc gccgcaaaat tgcagcagag
     2761 tgcacttgcg cttgaagaaa aggtcaacga gtcggcagaa atggcccgcc ttgccaacgt
     2821 cgaggctgag cgcgccaatc gcatcaagca ggcgctcgat gtagccagca ccagcgtcat
     2881 gatgaccgat gcacaaaaca atatcaccta taccaatgct gccctggaca gcatgatgcg
     2941 cgagcacgaa gccgacattc gcaaggcact gccccagttc cgggccaatg cgctcatggg
     3001 tgccaatatg gaccagttcc accgtaaccc tcagcaccag cgcaacatca ttggcaacct
     3061 gcacggcact cacgaagccc
//
LOCUS       DASUEG010000107         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_614197, whole genome shotgun sequence.
ACCESSION   DASUEG010000107 DASUEG010000000
VERSION     DASUEG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614197"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFX11_01885"
     CDS             complement(<1..442)
                     /locus_tag="VFX11_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4937393.1"
                     /translation="MKKQLCMLMASALVSTAAVADMGEGGYVGFDLSLVQGEMSIDEV
                     PGSFDFNPVALRVKGGGTFNKYFGLEGYIGMSVQGDALEQSDYDLKADNLIGLDALCI
                     LPVGNVLGLYGKVGVASFEYEDDYNDGYRESGLSYGVGAKTYLGS"
     gene            complement(489..1406)
                     /locus_tag="VFX11_01890"
     CDS             complement(489..1406)
                     /locus_tag="VFX11_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4937394.1"
                     /translation="MTDLILHHYPMSPFSEKVRVMLGYTQQPWLSALTREMPPRPLLE
                     QLAGGYRKIPVAQIGADIFCDSRTIAAEIAARSGKPELALENCSAEVQAYVAKVDLDV
                     FFACILAGGSKKLNQKVRQTMSWLDIARFFWDRINMGRKASVKIVGLREARPKALEHI
                     AQVEQRLQQDFLFGSQPNHADFSTYHSLWFIRDLGESRMINPYPKTIAWMDRMKAFGH
                     GARREIDPQQTLEIAKNATPRPIAAEHRTDPLIGKPVQIAPADYGQVPSVGTLAGSTP
                     AQWILARDVKGLGTLHVHFPKPGFMLTPL"
     gene            complement(1543..>2091)
                     /locus_tag="VFX11_01895"
     CDS             complement(1543..>2091)
                     /locus_tag="VFX11_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX4937395.1"
                     /translation="AYTLRLILDQIDSVNFPRSGWSGGARLFDSNPDLGADNPYTKWD
                     ADGVAVYSWRAHTFQFAAKAGGKIGENSLPRYDQFQWGGFLQQSGYATGQLLGESLQY
                     GRIMYYHQIMRGTFMDGAYSGFSLEATEMGEPLVPGNSTDVLKSVGIFLGTDTPIGPA
                     YLGYGLAEDGNEAFYFYLGKPY"
BASE COUNT          428 a          638 c          602 g          423 t
ORIGIN      
        1 tggagcccag ataggtcttg gcaccaacac cgtaggaaag cccgctctca cgatagccgt
       61 cgttgtaatc gtcctcgtat tcgaacgagg ctacaccgac cttgccatac aggcccagaa
      121 cattgcccac gggcagaatg caaagggcgt ccaggccgat caggttgtcg gctttgaggt
      181 catagtccga ctgctccagc gcgtcgccct gcacgctcat tccgatgtag ccttccaggc
      241 cgaagtactt gttgaaggta ccaccgcctt tcacgcgaag tgcaacgggg ttgaagtcaa
      301 aactgcccgg cacttcatcg atcgacattt cgccctgcac caaggacaga tcgaatccca
      361 cataaccgcc ttcacccatg tccgccactg cagctgtcga taccagcgcc gatgccatga
      421 gcatacagag ttgttttttc acgttctctc cctacctttc cggttttctc tttgatgtta
      481 ttgatgtctt acagcggtgt cagcataaac ccgggcttcg gaaaatgcac atgcagcgtg
      541 cccagccctt tcacgtcccg cgccagaatc cactgcgccg gcgtgctgcc cgccagggtg
      601 ccgacgctgg gcacctgtcc gtaatccgcc ggcgcgatct gcaccggttt gccgatcagc
      661 gggtcggtgc ggtgttcggc cgcaatcggg cgcggtgtgg cgttcttcgc gatttccagc
      721 gtctgctgcg gatcgatctc gcgacgcgcg ccatggccaa acgccttcat gcgatccatc
      781 caggcgatgg ttttcgggta ggggttgatc atgcgggatt cccccagatc gcggatgaac
      841 cacaggctgt gatacgtgga gaaatcggcg tgattgggct gggaaccaaa caggaaatcc
      901 tgctgcaggc gctgctccac ctgcgcgata tgttccagcg ctttgggccg tgcctcgcgc
      961 aggccgacga tcttgaccga cgccttgcgg cccatgttga tgcggtccca gaagaaacgg
     1021 gcaatgtcca gccaggacat ggtctggcgc accttctggt tcagcttttt gctgccacct
     1081 gccaggatgc aggcaaaaaa cacatccaga tccactttgg ccacgtaggc ctgcacctca
     1141 gcgctgcagt tttccagcgc cagctccggc ttgcccgagc gcgccgcgat ttccgcggcg
     1201 atggtgcggg aatcgcaaaa aatatcagcg ccgatctgcg ccaccggaat cttgcggtaa
     1261 ccgcccgcca gttgctccag caaggggcgt ggtggcatct cgcgggtgag cgccgacagc
     1321 cagggctgct gcgtataccc cagcatgaca cggacttttt ccgagaaggg ggacatgggg
     1381 taatggtgca ggatcagatc ggtcatggcg gctccagaca ttgcagggtg atcgcgggat
     1441 ggccgcagtg tgtggtgccg cagggaagcg gtcaacggcg aaatcggcca atattgctgc
     1501 cagaaccaaa gctagtcctt gcccggttta gcccgttgcg catcagtagg gtttacccag
     1561 atagaagtaa aaggcttcgt taccatcctc cgccaggcca tagcccagat aggccggacc
     1621 gatgggcgtg tcggtgccca gaaaaatgcc gacggatttg agcacgtccg tagaattgcc
     1681 cggcaccagc ggctctccca tctcggttgc ttccagcgaa aaaccgctat aggcaccgtc
     1741 catgaaagtg ccgcgcatga tctggtggta gtacatgatg cggccatatt gcaggctttc
     1801 gcccaacagc tggcccgtgg cataaccaga ctgctgcaga aaaccgcccc attgaaactg
     1861 atcatacctg ggcaaggaat tctcaccgat cttgccaccg gctttcgccg cgaactggaa
     1921 cgtgtgcgca cgccaggaat acacggcaac cccatccgca tcccacttgg tatagggatt
     1981 gtccgcaccc agatccgggt tggaatcaaa gagtctggcg ccgccgctcc agccggagcg
     2041 gggaaagttg acactgtcga tctggtccag aatcaggcgc agggtatagg c
//
LOCUS       DASUEG010000108         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_636062, whole genome shotgun sequence.
ACCESSION   DASUEG010000108 DASUEG010000000
VERSION     DASUEG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636062"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFX11_01900"
     CDS             complement(<1..463)
                     /locus_tag="VFX11_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX4937396.1"
                     /translation="MKFVSFNANGIRARLHQIEALLKLHTPDVIGIQETKVEDGQFPL
                     AELQGMGLHVEFFGQKSHYGVALLSRQPPTNVHKGFNGGDDEQKRLIRADYALPDGKS
                     VTVLNGYFPQGESRDHPVKFPNKEKYYAGVRQLLDERFQPAHNLILMGDMNV"
     gene            695..1345
                     /locus_tag="VFX11_01905"
     CDS             695..1345
                     /locus_tag="VFX11_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4937397.1"
                     /translation="MTDLSHISIFEGLPEKELAALQELCITRTYPKNTVIINEGDQAN
                     AMFICTGGKVKVYVSDENGREFVLNSMGPDEYFGELSLLDDDTRSASVITTDKSTFSI
                     LYKDDFTKVIMNNPQIALVLLRNLAQRIRQLTGNVKTLALQDVYGRIRKILMDLSTEK
                     EGETVIEEKLTQQDIANRIGSSREMVARILKDLATGGYIEIEKKQFRILKKLPESY"
     gene            1483..2172
                     /locus_tag="VFX11_01910"
     CDS             1483..2172
                     /locus_tag="VFX11_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937398.1"
                     /translation="MTNDIRMFRAAAAAGLLVAAIGIVLVIVYPQRLETLPPGYYTPI
                     LAIEFVTSLPEAQTLFANNATLVARTQTGHWVDMAFLFAHGAFLALANLGFWKRQRRP
                     LNLVGVFAAMIAASADLAENQYLLQLGEALQGLSAPPDFWLLRLYVTTKFLGICVAML
                     CLSLPLWQQGRVGKAFALVTLVLVPVSLGGVSGLPTLTEAMAGLIALGWVLLLVWLLR
                     TGKNLPPATPA"
     gene            complement(2179..2820)
                     /locus_tag="VFX11_01915"
     CDS             complement(2179..2820)
                     /locus_tag="VFX11_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937399.1"
                     /translation="MSALLLLADSQLLFRPDQLPQLLRHCSGQERSTAKPLSAAYIGA
                     ANGNRPEFFDLARLALATLFGQPVPCHFIQSESDIPPGPCALVILAGGSVETGWAFLQ
                     QQPVWDWLETCRSEADAVFIGVSAGAIHLASGCDPEQPSPRAQTYLGWAPLFVAVHEE
                     GENWPTARIWREAKSLQASGSLTFAGVPMGGGLWLDDGGCEPVGREGPLFYKP"
     gene            complement(2817..>3598)
                     /locus_tag="VFX11_01920"
     CDS             complement(2817..>3598)
                     /locus_tag="VFX11_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4937400.1"
                     /translation="RLDVLIIAGDATDRLDKLEYLFDQLVPCFYRVMFVPGNHELWIR
                     RSDFADSVEKFNAIQALCHHYGVQTEPLRLGQYQHVWLVPLQSWYDDKQFAGHTLFME
                     KTSALDRTDDMWGDFFHTRWPASIEVPLAQWFAQHNEPFLNWNYDAPVISFSHFLPRQ
                     ELIFGSDFNRSLLAKMFDPHPEFNFTRVAGSLRIHDQIRRLGSQVHVYGHQHRNRVRE
                     LDGVTYVSHCMGYPRERQMGFVKADAREPRCLWREDTGFCI"
BASE COUNT          728 a         1080 c         1057 g          733 t
ORIGIN      
        1 ccacgttcat gtcacccatc aggatcaggt tgtgggccgg ctggaagcgt tcatccaata
       61 actgtcgcac gccggcgtaa tacttttcct tgttgggaaa ttttacggga tggtcgcggc
      121 tttcgccttg aggaaaatag ccgttgagga cggtgacgga tttgccgtcc ggcagcgcat
      181 aatcggcgcg gatcaggcgt ttctgctcgt cgtcgccacc attgaaaccc ttgtgcacgt
      241 tggtgggcgg ctggcgtgac agcaaggcca caccgtaatg gcttttctgg ccgaagaact
      301 cgacatgcag gcccatgcct tgcagttccg ccagcggaaa ctggccgtct tccaccttgg
      361 tttcctggat tccaatcacg tcgggcgtgt gcagcttcaa cagcgcttca atctggtgca
      421 ggcgggcgcg aatgccgttg gcattgaagg atacgaattt cattttcttt ccccgggatg
      481 agccaatctg gttcagcgca gtttatcagt ccatcaccaa agatcacctg caatcttggc
      541 ggtgtcaggc cacaacgatt cgtcgcgcgg cggtgagtgt agactttctt tacacagttg
      601 aagccactac aatgatcaag ctgcatacca acaaaaaact cttccgctcg ctcaatccag
      661 attgagtaaa aaatcgccta acagggtgtt tcgcatgacc gatctcagtc acatttccat
      721 ctttgaaggg ctgcccgaaa aggagttggc ggcactgcag gagttgtgta ttacccgcac
      781 ctatcccaag aataccgtga tcatcaatga aggtgatcag gccaatgcca tgttcatctg
      841 caccggcggc aaggtaaagg tgtatgtgag cgatgaaaat ggtcgcgagt tcgtactcaa
      901 ttccatgggg cctgacgagt actttggcga actgtcgctg ctggacgatg acacccgttc
      961 tgcctcggtc atcaccaccg acaagtccac cttttcgatc ctgtacaagg acgacttcac
     1021 caaggtgatc atgaacaatc cgcagatcgc cctggtcctg ctgcgcaacc tggcccagcg
     1081 tatccgccag ctgaccggca acgtgaagac gctggcactg caggacgtat atggccgcat
     1141 ccgcaagatc ctgatggacc tgtcgaccga gaaggaaggc gaaaccgtca tcgaggaaaa
     1201 gctgacccag caggatattg ccaaccgcat tggctcgtcc cgcgagatgg tggcgcgcat
     1261 cctcaaggat ctggccaccg gcggttacat cgagatcgag aaaaagcagt tccgcatcct
     1321 gaaaaagctg cccgagagtt actgaggcac gcaattcaaa tcgacacggg acacggatgt
     1381 ccccgcatct gccctccatt tcatccggtt caatcgcatt tgccgactgt gttatggtta
     1441 cggcaaaaag tatgacacca cgatgacggg gacctccgtt caatgacgaa tgacatccgg
     1501 atgtttcgag ccgccgccgc cgccggcttg ctggtggcgg ccattggtat tgtgctggtg
     1561 atcgtttacc cgcaacggct ggaaacgctg cctcccggct actacacccc cattctggcc
     1621 atcgaattcg tgacttctct gccagaagcg cagaccctgt tcgccaacaa cgccaccctg
     1681 gtggcgcgga cacagaccgg ccactgggta gacatggcat ttctgttcgc ccacggcgcc
     1741 tttctggcac tggccaacct gggattctgg aaacggcagc gacgtccctt gaatctggtg
     1801 ggcgtgttcg cggccatgat cgcagccagc gcggatctgg ccgagaatca gtatctgctg
     1861 caactgggtg aagcactgca ggggttgtcg gctccgccgg atttctggtt gctgcgtctg
     1921 tatgtgacga ccaaattcct cggcatctgc gtggcgatgc tgtgtctgtc gctgccgcta
     1981 tggcagcagg gccgggtcgg gaaagcgttt gcactggtga cgctcgtgct ggtgccggtg
     2041 agcctgggtg gcgtcagtgg cctgccgacg ctgaccgagg cgatggcggg gctgatcgca
     2101 ctgggctggg tattgctgct ggtatggctg ctgcgtacag gcaagaatct gccgcccgcc
     2161 accccggcct gacctcggtc agggcttgta aaagagcggt ccctcccgac cgaccggttc
     2221 gcacccgccg tcatccaacc agagccctcc tcccattggc acgccggcaa aagtcaggct
     2281 gccggacgcc tgcaacgatt ttgcttcccg ccagatccgg gcagtcggcc agttctctcc
     2341 ttcttcatgc acggccacga acagtggcgc ccagcccagg tacgtttgcg cgcgcggcga
     2401 tggttgctcc ggatcgcaac cgctggccag atgaatcgcg ccggccgaca cgccgatgaa
     2461 caccgcatcc gcttcactgc ggcaggtttc cagccagtcc cacacgggtt gctgttgcag
     2521 gaacgcccag ccagtctcga cactgccgcc cgccaggatc accagcgcac aaggtcccgg
     2581 cgggatgtcg ctttccgact gaatgaaatg acagggcacc ggctgaccga acaacgtcgc
     2641 cagtgccaag cgtgcgagat caaaaaactc cggccggttg ccattggcgg caccgatata
     2701 ggcggccgac agcggcttcg ctgtggagcg ctcttgtcca gagcaatggc gcagcagctg
     2761 cggcaactgg tctggccgga acagcagctg gctgtcggcc agcaggagca gcgcgctcat
     2821 atgcagaatc cggtgtcctc gcgccagagg cagcgcggtt cccgggcgtc ggccttcacg
     2881 aatcccatct ggcgctcgcg cggatagccc atgcagtgcg acacataggt cacgccgtcc
     2941 agttcgcgca cccggttgcg gtgctggtgg ccgtacacat gcacctggct gcccaggcga
     3001 cggatctgat cgtgaatgcg cagcgatccc gccacgcggg tgaaattgaa ttccgggtgc
     3061 ggatcgaaca tttttgccag caggctgcga ttgaagtccg atccgaaaat cagctcctgg
     3121 cgcggcagga aatggctgaa gctgatcaca ggcgcgtcgt aattccagtt caggaatggt
     3181 tcgttgtgtt gcgcgaacca ctgcgccagc ggcacttcaa tggacgccgg ccagcgggtg
     3241 tggaagaaat ccccccacat gtcgtcggtg cggtccagtg ccgaggtctt ttccataaac
     3301 agcgtgtgcc cggcgaactg cttgtcgtcg taccaggatt gcagcggcac cagccacaca
     3361 tgctggtact gccccagccg cagcggttcg gtctgcacgc cgtagtggtg gcacagggcc
     3421 tggatggcgt tgaatttctc caccgaatcg gcgaaatccg accggcgaat ccagagttcg
     3481 tgattgcccg gcacgaacat gacgcggtag aaacagggca ccaactgatc gaacaggtat
     3541 tccagcttgt ccaggcgatc ggtggcatcg ccggcgatga tcagcacgtc cagccggt
//
LOCUS       DASUEG010000109         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_503649, whole genome shotgun sequence.
ACCESSION   DASUEG010000109 DASUEG010000000
VERSION     DASUEG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503649"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFX11_01925"
     CDS             complement(<1..224)
                     /locus_tag="VFX11_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937401.1"
                     /translation="MDFFLSDEIEQTRLQVRDFVQKEVIPLESNPANFDEHESLLEPI
                     VAQLREKAKAQGLWGFQLPKMYGGRELGVVG"
     gene            complement(281..1051)
                     /locus_tag="VFX11_01930"
     CDS             complement(281..1051)
                     /locus_tag="VFX11_01930"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4937402.1"
                     /translation="MSDINFRLDGKVVLITGASSGFGQFFAPALARQGAKVVVAARRR
                     NPLLALEEKIESDGGKCLAVAMDVTDRASVKDALDQVEAAFGCVDVLINNAGIGDSNA
                     MFDVTAEEWDRVMSTNLTGAFNVAQESARRMNQHGKHGSIINIASVLGLRVASHLTAY
                     CASKAGLIQLTKAMALELARYQIRVNAICPGYFLTDINRDFMETDAGKKMIQRVPQRR
                     LGELSELLGPLFLLASDASSFMTGSILSVDGGHAVSTL"
     gene            1255..>2311
                     /locus_tag="VFX11_01935"
     CDS             1255..>2311
                     /locus_tag="VFX11_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX4937403.1"
                     /translation="MSQLNPRQSEAVKYIDGPLLVLAGAGSGKTSVITRKIAYLIEQC
                     DLPAHRIVAVTFTNKAAREMKERVSQLVKGKQARGLTISTFHNLGLNIIRKELKLLGL
                     KNGFSIFDDQDSRTLLAELLHRETDVGGEDVDYIRNTISSWKNDLVLPAIAQSRAVDE
                     AELRAARTYEQYNRMLRAYNAVDFDDLILLPTLLFRDNDEARERWQHKIHYMLVDEYQ
                     DTNTSQYLLVRLLVGKRARFTVVGDDDQSIYAWRGARPENLAQLAQDYPALKVIKLEQ
                     NYRSTGRILKAANQVIANNPHVFEKQLWSEHGIGEHIRVVRCRSEDHEAEKIASDILN
                     QHLQKKRQFRDFAVLYRG"
BASE COUNT          502 a          723 c          660 g          426 t
ORIGIN      
        1 cccaccacac ccagctcacg gccgccgtac attttcggca gctggaagcc ccacaggccc
       61 tgcgcttttg ccttttcccg cagctgcgcc acgatcggtt ccagcaggct ttcgtgctca
      121 tcgaaattcg ccggattgga ttccagcgga atcacttcct tctgcacgaa gtcgcgcacc
      181 tgcagccggg tctgttcaat ttcgtccgac aaaaagaaat ccatgttgtt tccctcttca
      241 aaatgccaat gcaaaaaatg ccaacaataa gaaaaaaaac ttacagtgtg ctgaccgcgt
      301 gaccaccatc caccgacagg atcgagccgg tcatgaagga cgatgcgtca gaggccagca
      361 ggaacagagg gcccagcagt tcgctcaatt cgcccaggcg gcgctgcggc acgcgctgga
      421 tcatcttctt gccggcgtcg gtttccatga aatcgcggtt gatgtctgtc aggaaatagc
      481 cgggacagat ggcgttgacg cggatctgat aacgggccag ttccagcgcc atggccttgg
      541 tgagttggat caaaccggct ttcgaggcgc aataggcagt gaggtggctg gctacccgca
      601 ggcccagcac cgaggcgatg ttgatgatgc tgccgtgctt gccatgctgg ttcatgcggc
      661 gggcgctttc ctgcgccacg ttgaatgcgc cggtgaggtt ggtggacatg acccggtccc
      721 actcctcggc ggtgacatcg aacatggcat tgctgtcacc gatgcccgca ttgttgatca
      781 gcacgtcgac acaaccgaag gcggcttcca cctgatccag cgcgtccttc accgaagcgc
      841 gatcggtgac gtccatggcc acggccaggc acttgccgcc gtccgactcg attttttcct
      901 ccagtgccag caggggattg cgccgccgcg ccgccaccac cactttcgcc ccctgccgcg
      961 ccagcgccgg cgcgaaaaac tggccaaagc cgctggatgc accggtaatc agcacgacct
     1021 tgccatcgag ccggaaattg atgtcgctca tgtgtttccc cctgctgcta aactgctgtt
     1081 ttctggatga ttatgtcagc cgcggacggc cgtgacccac gggccacccc acgacgggag
     1141 cggcaagcat acgccaaagc cccagcccga tccaatcgac ggaactgaca gtttggtatc
     1201 caaaattaac agcaggcagg tacaatttcg ccccccgccc gagagagcac gcccgtgagt
     1261 cagttgaatc cccgtcagtc cgaagccgtg aagtacatcg acggacccct gctggtgctg
     1321 gcgggcgccg gatcgggcaa gaccagcgtg atcacccgca agatcgcgta cctgatcgag
     1381 cagtgcgacc tgcccgccca tcgcatcgtc gccgtcacct tcaccaacaa ggcggcgcgg
     1441 gaaatgaagg aacgggtgag ccagctggtg aagggcaagc aggcacgcgg cctcaccatt
     1501 tccacgttcc ataatctggg cctcaacatc atccgcaagg aactgaagct gctgggtctg
     1561 aaaaacggct tctcgatttt cgacgaccag gattcgcgca ccctgctggc agaactgctg
     1621 caccgggaaa cggacgtggg cggcgaggat gtggattaca tccgcaatac gatttccagc
     1681 tggaagaacg atctggtgct gccggccatc gcgcagtcac gggcagtgga cgaggcggaa
     1741 ctgcgcgccg cccgcaccta cgaacagtac aaccgcatgt tgcgcgccta caacgcggtg
     1801 gatttcgacg atctgatttt gctgccgact ttattgttcc gcgacaacga cgaagcgcgc
     1861 gaacgctggc agcacaagat ccactacatg ctggtggatg aataccagga caccaacacc
     1921 tcgcaatatt tgctggtgcg gctgctggtg ggcaagcgcg cccgcttcac cgtggtgggc
     1981 gacgacgatc agtccattta cgcctggcgc ggcgcccggc cggaaaatct ggcgcagctg
     2041 gcacaggatt atccggcgct gaaagtgatc aagctggaac agaattaccg ctccaccggc
     2101 cgcatcctga aagccgccaa ccaggtgatc gccaacaatc cccacgtgtt tgaaaagcag
     2161 ttgtggagtg aacacggcat cggcgaacac atccgcgtcg tgcgctgccg cagcgaggat
     2221 cacgaggcgg aaaaaatcgc ttccgacata ctgaaccagc atctgcagaa gaagcggcag
     2281 tttcgcgact tcgccgtgct gtatcgcggc a
//
LOCUS       DASUEG010000110         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1519608, whole genome shotgun sequence.
ACCESSION   DASUEG010000110 DASUEG010000000
VERSION     DASUEG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519608"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /gene="pepP"
                     /locus_tag="VFX11_01940"
     CDS             complement(<1..1176)
                     /gene="pepP"
                     /locus_tag="VFX11_01940"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX4937404.1"
                     /translation="MKISKKEFAERRRELMALMEPNSIAIVPAAPARTRSRDTEYPYR
                     QDSDFYYLSGFGEPEAVLVLVPGREHGEYVLFCRERDRAMEIWNGFRAGPEGAVKDFG
                     ADDAFPIGDLDEILPGLIEGRERVYYLMGRDPEFDRQVMEWVNTIRAKVRSGAHPPGE
                     FIALDHLLHDMRLFKSPAEVRIMQEAADMTASAHLRAMEKCHPGMMEFQLEAEFLHEF
                     SRNGSRFPAYSSIVGGGSNACVLHYIDNNCELKDGDLVLIDAGAEYQHYASDITRTFP
                     VNGKFSREQRALYDVVLKAQLAAIKQVKPGNHWNQPHEAALKELVKGLVQLGLLQGKV
                     DELIASEAYRRFYMHRTGHWLGMDVHDVGDYKVGDEWRVFEPGMVLTVEPGLYVAADD
                     "
     gene            complement(1219..1794)
                     /locus_tag="VFX11_01945"
     CDS             complement(1219..1794)
                     /locus_tag="VFX11_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX4937405.1"
                     /translation="MQPEDILDYYELEAELGKIQALSSPSEAHGILCGQLSGGVKAEG
                     LAWLKLYLPNLGVKREPWEDSREWFYQLRNFTLEELADDQFNFMPLLPDDEDPLEQRL
                     TSLAEWCSGFLAGFGSTGERRKEDFSEDTLMALRDLQQISQVDTDVEEEEMGENAFYE
                     VLEYVRMAALMLFTEFALDRNRMIPPSTTLH"
     gene            1959..2174
                     /locus_tag="VFX11_01950"
     CDS             1959..2174
                     /locus_tag="VFX11_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HEX4937406.1"
                     /translation="MNKIEQLFKNLENRVDEFIDLCDEYKRHNKFLKTREVQLTEERA
                     NLMKKNDMARTKVESMISRLRALEQDS"
     gene            2171..2485
                     /locus_tag="VFX11_01955"
     CDS             2171..2485
                     /locus_tag="VFX11_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX4937407.1"
                     /translation="MSTPLETLNVTILDRDYGVSCPAEEVEDLRTSAKLLDERMREIR
                     KSGKIVGIERIAVMAALNIAHELIRAKAELNSYDRITEKHLSRLNDKIERALASARQL
                     DL"
     gene            2584..2765
                     /gene="ssrS"
                     /locus_tag="VFX11_01960"
     ncRNA           2584..2765
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFX11_01960"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2811..3488
                     /locus_tag="VFX11_01965"
     CDS             2811..3488
                     /locus_tag="VFX11_01965"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX4937408.1"
                     /translation="MTDNFTAPAHTPHLPPAITQDLTPPDGAVPGKNSLRKTLRQRRR
                     QLTRQQQQIASMGLCRQLVQLPVFVNSQRIAAYIPNDGEINPQPLLELAWRLNKRIYL
                     PVLHPFKKGHLFFMEYRPHQPLAINRFGIPEPLCDHDTRCPAWTLDLVLTPLVGFDEQ
                     GNRMGMGGGFYDRTFAFMQTGHTPRRPRMVGVAHECQKVPALPCERWDIGMDFIVTDR
                     KIYGTSN"
     gene            complement(3538..3663)
                     /locus_tag="VFX11_01970"
     CDS             complement(3538..3663)
                     /locus_tag="VFX11_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937409.1"
                     /translation="MKKKPDLLLVLVLVLGLGVLASSYTQGEPDPEMVASQFTIR"
BASE COUNT          814 a         1197 c         1068 g          784 t
ORIGIN      
        1 gtcatcggca gccacgtaca ggcccggctc caccgtcagc accatgcccg gttcgaacac
       61 gcgccactcg tcacccacct tgtagtcgcc cacgtcatgc acgtccatgc ccagccagtg
      121 accagtgcgg tgcatgtaaa aacggcgata cgcttccgac gcaatcagtt cgtccacctt
      181 gccctgcagc aggcccagct gcaccagacc cttcaccagt tccttcaggg ccgcttcgtg
      241 gggctgattc cagtgattgc caggcttcac ctgcttgatc gccgccagct gcgctttcag
      301 caccacgtcg tacaatgcgc gctgctcgcg actgaacttg ccgttcaccg gaaacgtacg
      361 cgtgatgtcg gacgcataat gctggtattc ggccccggcg tcgatcagca ccagatcgcc
      421 gtctttcagt tcgcaattgt tgtcgatgta atgcagcacg caggcattgc tgccaccacc
      481 cacgatactg gaataggcag gaaaacggct gccgttgcgg gaaaattcgt gcaggaattc
      541 cgcttccagc tgaaactcca tcatgcccgg atggcatttt tccatcgcac gcaaatgggc
      601 gctggcggtc atgtccgctg cttcctgcat gatgcggact tctgccgggc ttttgaaaag
      661 gcgcatgtcg tgcagcaggt gatccagcgc gatgaactcg ccaggcggat gcgcgccgct
      721 gcgcaccttg gcgcggattg tgtttaccca ctccatcacc tgacggtcaa attcgggatc
      781 acgacccatc aggtaataca cgcgctcgcg accctcgatc aggcccggca ggatctcgtc
      841 cagatcgccg atgggaaacg catcgtcagc accgaaatct ttcactgcac cttccggccc
      901 tgcgcgaaag ccgttccaga tttccatggc gcgatcgcgc tcgcggcaga acagcacgta
      961 ttcgccgtgt tcacggccgg gtaccagtac cagtacggct tcgggctcgc caaagccgga
     1021 caggtagtaa aagtcactgt cctggcggta gggatattcg gtgtcgcggc tgcgggtgcg
     1081 ggcgggggcg gcgggaacga tggcaatgct gttgggttcc atcagcgcca tcagttcacg
     1141 gcggcgctcg gcgaattctt tcttgctgat tttcatagac acctttattc acggacacct
     1201 tgtcaggcgg agcaacactc aatgcagtgt agtggacggc ggaatcatgc ggttgcggtc
     1261 cagcgcgaat tcggtgaaca gcatcagcgc cgccatgcgc acatattcca gcacttcata
     1321 gaaggcgttc tcgcccattt cctcttcttc cacgtccgtg tccacctggc tgatctgctg
     1381 caggtcgcgc agtgccatca gggtatcctc gctgaaatcc tctttcctgc gctcgccggt
     1441 actgccgaaa ccggccagaa agccggagca ccattccgcc agggacgtga gacgctgttc
     1501 cagcgggtct tcgtcatcgg gcagtagagg catgaaattg aactggtcgt ccgccagctc
     1561 ttccagggtg aaattgcgca gctggtagaa ccattcacgg gaatcttccc agggttcgcg
     1621 cttcaccccc aggttgggca ggtacagctt gagccatgcc aggccctccg ccttcacccc
     1681 cccgctcagc tggccgcaca ggatgccgtg ggcttccgag gggctgctca gggcctggat
     1741 cttgcccagt tccgcttcca gttcgtaata gtcgagaatg tcttccggtt gcacggacag
     1801 ggtacctttg cagttttgta acaggacgga gcattgtaac acgcacggac tggctgcaaa
     1861 ggcaggattg gggctatgct tgcgatggag aacgtcacat cctttcgtgt aaaataacaa
     1921 gaatgccggc caaggtccgg cgcggaaacc cgacccccat gaacaagatt gagcagcttt
     1981 tcaagaatct ggaaaaccgc gttgacgagt tcatcgatct ctgtgacgag tacaagcgtc
     2041 acaacaaatt cctgaagaca agggaagtcc aactgaccga ggaacgggcc aatctgatga
     2101 agaagaacga tatggcacgc accaaggtcg aatccatgat ttcgcgatta agagctctgg
     2161 agcaggattc atgagcaccc ccctggaaac attgaacgtc accattctgg accgggacta
     2221 cggcgtcagc tgtccggccg aggaagtgga agatctgcgc acctccgcca aactgctgga
     2281 tgagcgcatg cgggaaatcc gcaaatccgg caagatcgtc ggcatcgagc gcatcgcggt
     2341 gatggcggca ttgaacatcg cccacgagct gatccgggcc aaggccgaac tgaacagcta
     2401 cgaccgcatt acggaaaaac acctgtcacg cctcaacgac aagatcgagc gggcgctggc
     2461 gtcagcgcgt cagctggacc tgtaaaggcc gcacgcggct actcctattc aagccaaaat
     2521 tcaatcaatc aattttcccc ggccccgctg tccgcgccgc caaatccgtt atactgaaat
     2581 ctcagccccc tgagctgttc gcgttctggc ctacgtccct gagccgatgc attaatttaa
     2641 aacgggggtc tcggctggtg tgggagtgca cgtccgcttg acggaaagcc cgaaacatca
     2701 cagaggccgc caccttgaac cttgggttca agggcagacg tcagtagcga cagatcgggg
     2761 ggcgcttgat ttttgctgca agcctgaact tcctgtcacc gaggctctgc atgaccgaca
     2821 atttcactgc gcctgcccat acgccccatc tgccacccgc cattacccag gacctcacac
     2881 cgcctgatgg cgccgtaccc ggcaagaaca gcctgcgcaa aaccctgcgt cagcgccgcc
     2941 gccagctgac ccggcagcaa cagcagatcg cctccatggg cctgtgccgt caactggtgc
     3001 agctgccggt attcgtgaac agccagcgca ttgctgccta cattcccaat gatggcgaga
     3061 tcaatcccca acctctgctg gaactggcat ggcgcctgaa caaacggatc tatctgccgg
     3121 tgctgcaccc gttcaaaaag ggccatctgt ttttcatgga ataccggccg caccagccgc
     3181 tggccatcaa ccgcttcggc attccggagc ccttgtgcga tcacgacacc cgctgcccgg
     3241 cctggacgct ggacctggtg ctgacaccgc tggtgggatt cgatgaacag ggtaaccgga
     3301 tgggaatggg gggcggcttt tacgaccgca cctttgcgtt catgcagacc ggccatacgc
     3361 cgcgccgtcc gcgcatggtg ggcgtggccc atgaatgcca gaaggtgccg gcgctaccct
     3421 gcgaacgttg ggacatcggt atggacttta ttgtgacgga caggaaaatc tacgggacca
     3481 gcaactgaga cggcactgca acagccggcg tcagttgtgg gaattgcagt gtcgtaatca
     3541 gcggatcgtg aactggctgg ctaccatttc cggatcgggc tcaccctgag tgtagctgct
     3601 cgccagcacg cccagaccca gcaccagtac cagtaccagc agcaaatcgg gtttcttctt
     3661 catctcgctc gttgcctcgt caaaccttgc catcctccct gttgaaacgg gtccacctcc
     3721 ctggtggtcc ggacggccga ttccatagcg ttatagttgt ttaaaagatg ccaggaatgg
     3781 gagcctgtct gtaatctgtt ccatacctgc gcgtacgcgg tacggatttc cttgccctaa
     3841 ctatagaaca gccctgacag cac
//
LOCUS       DASUEG010000111         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_503577, whole genome shotgun sequence.
ACCESSION   DASUEG010000111 DASUEG010000000
VERSION     DASUEG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503577"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            702..1964
                     /gene="glgC"
                     /locus_tag="VFX11_01975"
     CDS             702..1964
                     /gene="glgC"
                     /locus_tag="VFX11_01975"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX4937410.1"
                     /translation="MTKYADSSYSYLKNTLALILAGGSGSRLQSLTRWHSKPAVPFGG
                     KYRTIDFPLSNCINSGIRRISILTQYKSHSLNTHIQKGWNFLRPELGEFIDLLPAQQR
                     VKDSWYLGTADAVYQNLDIIDNIHPDYVLILAGDHIYKMNYALMLEHHINTGADMTVG
                     CIEVPVQQAREFGVMSVDEDNWITRFTEKPAHPETIPGRRNLCMASMGIYVFSRDFLY
                     QVLKRDAGQLSSHHDFGKDVIPSAVGQYKVAAFPFTDSATGDTAYWRDVGTVDSYYEA
                     NMELVSVTPPLNLYDQTWPIWTYQEQLPPAKFVFDDDGRRGMAVDSLVSAGCIVSGSE
                     VRRSLLSNNVRIHSYCEIDDAVILPNVEIGCHCRLRNVIIDRDCRIPPGTVVGFDPEE
                     DAKHFYVSGAGIVLISPEMLGQSLSNVA"
     gene            1954..3672
                     /locus_tag="VFX11_01980"
     CDS             1954..3672
                     /locus_tag="VFX11_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX4937411.1"
                     /translation="MSPDASTDSKPHPFPVNVVLCWHMHQPQYQDQHNRRFHLPWTYL
                     HAIKDYVDMAAVVEELPGCRVVVNFTPVLLDQLDDYIRQFDRFFNEGAVFDDALLQAL
                     VAPTIGKTEAQRLAVVKQCVRANENRLIRRFEPYHDLVQLARQALTKPGYLAYLDEQY
                     FFDLLVWYHLVWMGETVREHNATVQRLLCKARDFSPGDRRELLGVIDQLLRTIVPRYR
                     HLAENGQMELSVTPDKHPILPLLLDFRSALETMPHAPLPQSQSYPGGEDRARDHLERG
                     IAIFERHFGFRPVGCWPSEGALSQDTLRLLREAEFQWTASGGGVLGNSRNASGGGNIC
                     IHHPFRFDDCDLTCFFRDDNLSDLIGFTYSGWNEEDAVNNLLHHIRNIRAACGYAEHT
                     IVPIILDGENCWEHYAENGYPFLKTLYSRLSEDPEIHLTTFRDYLKTRPTPTRLTRLA
                     AGSWVYGTLSTWIGDEGKNLAWDLLCQAKLDFDRVMQAGTLSPEQADRAREQLAICEG
                     SDWFWWFGDYNPAESVADFDNLYRCHLKNLYRLLGEPAPPALEQIISQGGGSPENDGV
                     MRRGQG"
     gene            3676..5136
                     /gene="malQ"
                     /locus_tag="VFX11_01985"
     CDS             3676..5136
                     /gene="malQ"
                     /locus_tag="VFX11_01985"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX4937412.1"
                     /translation="MVNILKTRRAGVILHPTSLPGPQACGSLGADAVRWINWLAACGF
                     RLWQFLPLTPVSDGSPYNSYSAFAGNTQLIDIAALQELGFPPLTGVTLSPSAPDYVRT
                     ALQHQFDWFEHQQEGPLREQFRHFTQSQHWLEDYCLFSALKEVHPQHWVEWPAPLRDR
                     EPAALQAFATQYRQTIHFHRFAQFIFMTQWHRLRQHANGKGIALFGDMPIFVAHDSVD
                     VWSNRGLFKLDDQGNTTVIAGVPPDYFSQQGQRWGNPLYDWEQHAADGYGWWQQRIHH
                     GLSLYDAVRIDHFRGFLACWEIPASETTAVNGRWVPAPGDDLFNALLQARGELPLIAE
                     DLGVITPDVIELRKKYKLPGMKILQFAFDSDGANPYLPHNHSRDSVVYSGTHDNNTTL
                     GWFQAMNAEQKNHVLNYFAWPGEPMPWPIIKSALASPARWAILPLQDLLVLGAEHRMN
                     VPGTTTGNWRWRFSWHQVGDSLPTYLHSLNRIYHRI"
     gene            complement(5194..>6106)
                     /gene="glgP"
                     /locus_tag="VFX11_01990"
     CDS             complement(5194..>6106)
                     /gene="glgP"
                     /locus_tag="VFX11_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX4937413.1"
                     /translation="PGQEFIRQIHRYSRDPLFEGRIILLEDYDMSLARKLVTGVDVWL
                     NNPTYPLEASGTSGQKAGINGVLNLSVLDGWWDEGYNGENGWAITPHGSQFTPDFRDR
                     EESNELLDVLENQVIPLYYQRDGQGFSASWIDKSKHAMASLIPAFNAQRMVMDYVEGF
                     YGPAIAHGRLLCANHHEPARQLAEWKRRVRAAWPKVSIRRLDTPLNHIYRDDNFEVKV
                     AVNLDGLDPSDVVIDCLVGRQEKDAAFERHSCFQLQFVERDANGESIFSLAVTLTEPG
                     KQCYMLRAYPYHQLLGQRFETGCMIWL"
BASE COUNT         1264 a         1832 c         1759 g         1251 t
ORIGIN      
        1 ttttatgctt tgcgcttgaa tcattagctg gaccgtggcg tagcgcggga tgccattacg
       61 atcgtggata ctggcgtccg cggaacaatt gcatttatgc agggcatcgc cgatcagccg
      121 ttcatcatgc aacacccaat aattcgcatg catgaatggt gggcgcatgg acaccgcaat
      181 gcttccgacg cctgaagcgt tcgtcgaagc aacgtccctg cagtgggttg cggcggttgg
      241 ggaccgacgg catttttcct tcgattctca acctgtgcgc tatcaagcaa gatccgaacc
      301 accgcgccac gatcaactaa gccactgaaa tttataccat tcagccacaa gcacctggaa
      361 cctgttgcaa atcctgcaac agatcaaggc gagtaccgga attttttgcc aggatgctac
      421 gcccgcttgt aacgcagagc ctgacccatt tgcgcggaat gggtgcattg aacccggctt
      481 tgcgtcagtc aggttacagg cggattaatt gggaaaaaga agttgccgta gcacgcacca
      541 atatttattt ttatcgcggt gaagaaagac ttatgatgaa agcatcgcac aggagttaaa
      601 gcgatttggt agtccgacgc aattgcgtcg gggatttcga ggcagcaacg tcgcagaaaa
      661 ttttgaccag cgttgtatca cttggaacac atgggtacgt catgactaaa tacgcagatt
      721 ccagctattc ctatctgaaa aacaccctgg cgctgattct ggcaggcggt agcggttcac
      781 gcctgcagtc actcacccgc tggcactcga aaccggcggt gcctttcggc ggcaagtacc
      841 gcaccatcga tttcccgctt tccaattgca tcaattccgg catccgccgc atcagcattc
      901 tgacccagta caagtcccac tctctcaaca cccatatcca gaaaggctgg aatttcctgc
      961 gcccggagct gggtgaattc atcgacctgc tgccggccca gcagcgggtg aaggattcct
     1021 ggtacctggg caccgcggac gccgtgtacc agaacctgga catcatcgac aatattcatc
     1081 cggattacgt gctgattctg gccggcgacc atatctacaa aatgaactat gcgctgatgc
     1141 tcgaacatca catcaacacg ggtgccgaca tgacggtggg ttgcattgaa gttccggtgc
     1201 agcaggcacg ggaattcggc gtgatgtcgg tggacgagga caactggatc acacgcttca
     1261 cggaaaagcc ggcccatccg gaaaccattc ccggacgtcg caacctgtgc atggcttcca
     1321 tgggaatata tgttttttca cgggattttc tgtatcaggt tctgaaacgt gacgctggcc
     1381 agctcagcag tcatcatgat ttcggcaagg atgtgattcc ctctgcagta ggccagtaca
     1441 aggtcgccgc cttccccttc accgacagcg ccaccggcga cactgcctac tggcgcgacg
     1501 tgggcacagt ggacagctat tacgaagcca atatggaact ggtgtcggtg actccccccc
     1561 tgaacctgta cgatcagacc tggccaatct ggacctacca ggaacaattg ccaccggcca
     1621 agttcgtatt cgacgatgac ggccgacgcg gcatggcggt ggattcgctg gtgtcggcag
     1681 gctgcatcgt gtcgggcagt gaagtacgcc gttccctgct gtcaaataac gtgcgcatcc
     1741 acagctactg tgaaatcgac gacgccgtca tcctgcccaa cgtggagatc ggttgtcact
     1801 gccgcctgcg caacgtgatc attgaccgcg actgccgcat tccacccggc accgtggtgg
     1861 gattcgatcc ggaagaagat gccaaacatt tttacgtatc aggtgccggc atcgtgctga
     1921 tttcgcctga gatgctcggg cagtctttat ccaatgtcgc ctgacgccag cacggacagc
     1981 aagccgcacc cgtttcccgt taacgtggtg ctgtgctggc acatgcacca accccagtac
     2041 caggaccagc acaatcgccg gtttcacctg ccttggacct acctgcacgc catcaaggat
     2101 tacgtggaca tggcggccgt agtggaagaa ctgcctggat gccgggttgt ggtgaacttc
     2161 acgcctgtac tgctggatca gctggacgac tacatccggc agttcgaccg cttcttcaat
     2221 gaaggtgcag tgtttgacga tgcgctgctg caggccctgg tcgcgcccac catcggcaag
     2281 accgaggcgc agcggctggc agtggtgaag caatgcgtgc gcgccaacga aaaccggctg
     2341 atccgccgct tcgaacccta tcacgacctg gtgcagctgg cgcgacaggc gctgacaaaa
     2401 ccgggctacc tcgcgtatct ggacgaacag tactttttcg atctgctggt ctggtatcac
     2461 ctggtatgga tgggcgagac ggtacgcgag cacaatgcga ccgtgcaacg gctgctgtgc
     2521 aaggcgcgcg atttcagccc gggcgaccgg cgtgaattgc tgggcgtgat tgaccagttg
     2581 ctgcgcacga tagtgccacg ctaccggcac ctggcggaaa atggccagat ggaattgagt
     2641 gtgacgccgg acaaacatcc gatcctgccg ctgctgctgg attttcggag cgctctggag
     2701 accatgccgc acgcaccgct gccgcagagc cagtcctatc ctggcggtga agaccgtgcc
     2761 cgcgaccacc tggagcgggg cattgccatt ttcgagcgcc acttcggatt tcgcccggtg
     2821 ggatgctggc catcggaagg tgcgctcagc caggatacac tgcggctgtt gcgggaagcg
     2881 gaattccagt ggaccgccag tggtggtggc gtgctgggta acagtcgcaa cgccagtggt
     2941 ggcggcaaca tctgcatcca tcacccgttc cgcttcgacg actgcgacct cacctgtttt
     3001 tttcgcgacg acaatctttc agacctgatc ggcttcacct attccggctg gaacgaagaa
     3061 gacgcggtca acaatctgct gcaccacatc cgcaatatcc gtgcggcctg cggctatgcc
     3121 gaacacacca tcgtgcccat catcctcgat ggcgaaaact gctgggaaca ctatgcagag
     3181 aacggctatc cgttcctgaa aaccctttac agccgcctga gcgaagaccc agagatccac
     3241 ctcaccactt ttcgcgacta cctgaaaacc cggcccacgc ccacccgcct gacccggctg
     3301 gcggccggca gctgggtgta tggcaccctg tccacctgga tcggtgacga gggcaagaat
     3361 ctggcctggg atctgctgtg tcaggcaaaa ctggatttcg atcgcgtcat gcaggcgggc
     3421 acgctgtcac ccgaacaggc ggaccgggca agggagcagc tggcgatctg cgaaggatcg
     3481 gactggttct ggtggttcgg cgattacaac ccggcggaat ccgtggcgga tttcgataat
     3541 ctgtaccgtt gccatctgaa gaacctgtac cgtcttctgg gtgagccggc cccgccggcg
     3601 ctggagcaga tcatcagcca gggcggtggc agtccggaaa atgatggcgt gatgcggcgg
     3661 ggacaaggct gatacatggt gaacattctg aaaacgcgcc gcgctggcgt tatcctgcac
     3721 ccgacttcac tgcctgggcc gcaggcctgt ggcagcctgg gcgccgatgc agtgcgctgg
     3781 atcaactggc tggcagcctg cggttttcgc ctgtggcaat tcctgccgct gacgccggtg
     3841 tccgacggct ctccctacaa cagttattcc gcgttcgccg gcaacacgca gctgatcgac
     3901 atcgccgcac tgcaggagct gggcttccct cctctcaccg gcgtcacgct cagccccagt
     3961 gcacccgatt atgtgcgcac tgcgttgcag caccagttcg actggttcga gcaccagcag
     4021 gaaggtccgc tgcgtgaaca gttccggcac ttcacgcaat cccagcactg gctggaggat
     4081 tactgcctgt tctcggcact gaaggaagtg cacccgcagc actgggtgga gtggccggca
     4141 ccactgcggg accgggagcc agccgcgttg caggccttcg ccactcaata ccggcagacg
     4201 atccatttcc accgctttgc gcaattcatc ttcatgacgc agtggcaccg cctcaggcaa
     4261 catgccaacg gcaagggcat tgccctgttc ggcgacatgc cgattttcgt tgcacacgac
     4321 agcgtggatg tgtggagcaa tcgcggtctt ttcaagctgg acgaccaggg caacaccacg
     4381 gtgattgccg gtgtgccgcc ggactatttt tcccagcagg gccagcgctg gggcaatccg
     4441 ctgtacgact gggaacagca tgccgccgat ggatacgggt ggtggcagca acgcatccat
     4501 cacgggctgt cgctgtacga tgccgtgcgc atcgatcatt tcagaggctt tctggcctgc
     4561 tgggaaattc cggcgtccga aaccaccgca gtgaacggtc gctgggtacc ggcaccgggc
     4621 gacgatctgt tcaacgcgct gctgcaggcc cgcggagaac tgccactgat tgccgaggac
     4681 ctgggcgtga tcacgccgga tgtcatcgaa ctgcgcaaga aatacaaact gccgggcatg
     4741 aagatcctgc agtttgcctt cgacagtgac ggcgccaatc cctatctgcc gcacaaccat
     4801 tcgcgggatt cagtggtgta ttccggcacg cacgacaaca acaccacgct gggctggttc
     4861 caggcaatga atgcggagca gaaaaatcat gtactgaatt atttcgcctg gcccggcgag
     4921 cccatgccct ggcccattat caagagtgcg ctggcgtcgc cggcgcgctg ggccattctg
     4981 cccctgcagg atctgctggt gctgggtgcg gaacatcgca tgaatgtgcc gggcaccact
     5041 accggtaact ggcgctggcg cttcagctgg catcaggtgg gcgacagcct gcccacctat
     5101 ctgcacagtc tgaaccggat ctaccatcgg atctgaaccg cattgaacgt ctgaatccgc
     5161 tgccttgcag gaagcggagc cctgaggtca ccgtcagagc cagatcatgc agccggtttc
     5221 gaagcgctgt ccgagcaact ggtgatacgg ataggcccgc agcatgtagc actgcttgcc
     5281 aggttccgtc agtgtcactg ccagcgagaa aatggattcg ccgttggcat cgcgctcgac
     5341 gaactgcagc tgaaagcagg aatggcgttc aaacgcggcg tccttctcct gccggcccac
     5401 caggcaatcg attaccacgt cactgggatc gagcccgtcc agattcaccg ccactttcac
     5461 ttcgaaattg tcatcgcggt aaatgtggtt caggggcgta tccagccgtc ggatggaaac
     5521 cttgggccat gccgcgcgca cccgccgctt ccattccgcc agttgacgcg ccggttcatg
     5581 gtgattggcg cacagcaagc ggccgtgggc aatcgccggg ccgtagaatc cttccacgta
     5641 gtccatcacc atgcgttgcg cgttgaacgc cgggatcagt gatgccattg catgcttgct
     5701 cttgtcgatc cagctggcgg aaaaaccctg gccgtcacgc tggtagtaca gcggaatcac
     5761 ctgattttcc agtacgtcga gcaattcatt ggattcctcg cgatcgcgaa aatccggtgt
     5821 gaactgcgag ccgtggggcg taatcgccca gccgttttcg ccgttgtagc cttcgtccca
     5881 ccagccatcc agcacgctca ggttgagcac gccgttgatg ccggccttct gccccgaggt
     5941 gccgctggct tccaggggat aggtgggatt gttcagccat acatcgacac cggtcaccag
     6001 cttgcgtgcc agcgacatgt cgtaatcctc cagcaggatg atgcggcctt cgaacagcgg
     6061 atcgcgcgaa taacggtgga tctggcgaat gaattcctgt cccggg
//
LOCUS       DASUEG010000112         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_172302, whole genome shotgun sequence.
ACCESSION   DASUEG010000112 DASUEG010000000
VERSION     DASUEG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172302"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFX11_01995"
     CDS             <1..505
                     /locus_tag="VFX11_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4937414.1"
                     /translation="AIYWHFKNKVDLFNAMCDRVTLPLEAMTEAATRDDVADPLGELR
                     HSVRYFFHKVVTDEHYRLVFDILFNKCEFVAELGPIVERDSRVREEFRQRFERMLLNA
                     EKRGQLPAGLNHRLAVESYQAYVKGILRNWLLNPASFDLLVDGDRMLDAFFELLQVSR
                     ALRPGHS"
     gene            575..2407
                     /locus_tag="VFX11_02000"
     CDS             575..2407
                     /locus_tag="VFX11_02000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937415.1"
                     /translation="MKRIHTLLSLILLLMAVPLVQAASISLQPGFRQQNLAGVLAYLE
                     DREGTLALADLLQDDARFAWHISEQPNLNFGFTDAVYWARFDVDSPSTPQTQNLFLEI
                     SYPLLDDIRVYLLQDGTLLREFHVGAGLPFAERLVRHGNFLIPLDVSPQPLQVVLRVQ
                     SQSSMQIPLLLWEKNAFVEHTYAEATAFGFFYGTVLIIAVYNLLVFFSVREPGFLYFF
                     FYSTGIVIVEAALRGAAFAFVWPEAVNWVDRSIVVGIASCTLFGSLFTNNVLLVRQTR
                     PVISRWLQLVMVLSAACFVGAMVLPYKVMILVGLLVVTLSSILNPLAHALRVKDGYTP
                     ARYILVGSVVFILSIFVTIVSKVGWLPRNAFTEYTPYYGTVFQLLLFSLSLADRLNAD
                     RKLREEAQLKAAASQQELLGVQLKINEDLDRRVRERTDELEALNTKLQELSTTDSLTG
                     LRNRRYFDDMLKNEYQRAFRDKLPLSVLLIDIDHFKKLNDNFGHPFGDLCLVEAANLI
                     RGCIKRPPDVAARYGGEEFVVLLPQTDREGAVKVAESLRAAFNAKRIEDGPHSVVMTI
                     SIGVSSTVPPDRDGHEILLKRADALLYRAKQNGRNRVEWETGSA"
     gene            2459..>2857
                     /locus_tag="VFX11_02005"
     CDS             2459..>2857
                     /locus_tag="VFX11_02005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937416.1"
                     /translation="MASLAARAVKAYARRVIKKVPRDPEHLVQHLRRALNNSPLPNWL
                     PRGVRRTQFAVEGVRGEWLRVNNPTQVILYLHGGGYIAGVPTTYLTLCAKFAKALQAD
                     VYMPAYRLAPEHPFPAALDDAMTVYRLLLKK"
BASE COUNT          533 a          811 c          875 g          638 t
ORIGIN      
        1 cgccatctat tggcacttca agaacaaggt agacctgttc aacgccatgt gcgaccgggt
       61 gaccctgccg ctggaagcca tgacggaggc cgccacccgc gacgatgtgg ccgatcccct
      121 gggggaactg cgccattccg tgcgctattt cttccacaag gtggtcaccg acgagcatta
      181 ccggctggtg ttcgacatcc tgttcaacaa gtgcgaattt gtggcggaac tggggcccat
      241 cgtcgagcgc gattcgaggg tgcgtgagga attccgccag cgctttgagc gcatgctgct
      301 gaatgcagaa aaacgcgggc agctgccggc gggactgaat caccggctgg cagtggaaag
      361 ctatcaggcg tacgtgaagg gcattctgcg caactggctg ctgaatcctg ccagcttcga
      421 tctgctggtg gacggcgatc gcatgctgga cgccttcttt gagctgctgc aggtctcgcg
      481 ggcactgcgg cccggccaca gttaattcga acgcgcctgt ttacacgccc gtctacactg
      541 aagtcaggac caatgactgc tggtggggct gtctttgaaa cgcatccata cactgctctc
      601 cctgattctg ctgctgatgg ccgtaccgct ggtgcaggcc gcgtcgattt cgctgcagcc
      661 gggtttccgc cagcagaacc tggcgggtgt gctggcctat ctggaagaca gggagggcac
      721 gctcgcgctg gcggatctgt tgcaggatga tgcccgtttc gcctggcaca tttccgaaca
      781 acccaacctg aatttcggtt tcacggacgc cgtgtactgg gcgcgcttcg acgtggacag
      841 tccgtccaca ccgcagacac agaatctgtt cctggaaatc agctatccgc tgctggatga
      901 catccgggtg tatctgctgc aggacggtac cctgctgcgc gaattccatg tcggcgccgg
      961 cctgccgttt gcagagcggc tggtgcgcca cggcaatttt ctgatccccc tggatgtgag
     1021 cccgcagccg ttgcaggtgg tgttgcgggt gcaatcgcaa tcatcgatgc agattccgtt
     1081 gttgctgtgg gaaaagaatg cgttcgttga gcacacctat gccgaggcca ccgcgttcgg
     1141 atttttttat ggcacggtgc tgatcattgc ggtgtacaac ctgctggtat ttttctcggt
     1201 gcgtgaaccg ggattcctgt atttcttttt ctactccacc ggcattgtca tcgtggaagc
     1261 ggcgttgcgt ggtgcggcgt ttgcgttcgt ctggccagag gcggtgaact gggtggatcg
     1321 cagtattgtg gtgggtatcg ccagctgcac gctgttcggc agcctgttta ccaataatgt
     1381 gctgctggtg cgacagaccc ggccggtcat ttcgcgctgg ctgcagctgg tgatggtgct
     1441 gtcagcggcc tgcttcgttg gcgcgatggt attgccatac aaggtgatga ttctggtggg
     1501 cctgctggta gttaccctgt catcgattct caatcctctg gcgcatgcgc tgcgggtgaa
     1561 ggatggttat acaccggccc gctacatcct ggttggcagc gtcgtgttca tcctgtcgat
     1621 ttttgtcacc atcgtttcca aagtgggatg gttgccgcgc aacgctttca ccgaatacac
     1681 accctattac ggcacggtgt tccagttgct gctgttttcg ctgtcgctgg ccgaccgtct
     1741 gaatgcagac cgcaagctgc gggaagaagc gcagctgaaa gccgctgcca gccagcagga
     1801 attgctgggg gtgcagctga aaatcaacga ggatctggac cgccgggtgc gcgaacgcac
     1861 cgatgaactc gaagcgctca acaccaaact gcaggaactg agcaccaccg acagcctcac
     1921 cggtctgcgc aaccgtcgct atttcgacga catgctgaag aatgaatacc agcgcgcttt
     1981 tcgcgacaag ctgccactgt cggtgctgct gatcgatatc gatcacttca agaaactgaa
     2041 cgacaacttc ggccatccgt tcggtgatct gtgcctggtg gaagccgcca atctgatccg
     2101 tggctgcatc aagcggccgc cggatgtggc ggcccgttat ggtggtgagg agttcgtagt
     2161 gttgttgccg cagacagacc gcgaaggggc ggtgaaagtg gccgaaagtc tgcgtgctgc
     2221 gttcaatgcc aaacgcatcg aggatggccc gcacagtgtt gtcatgacga tcagcattgg
     2281 cgtgtccagc acagtcccgc cggatcgcga cggccatgaa atactgttga aacgcgccga
     2341 tgcgctgttg tatcgcgcca aacagaacgg ccgcaaccgc gtggagtggg aaaccggttc
     2401 cgcctgagcg atgctgttcc tataatggcg ccacccgaac ctcataccag gagtcgccat
     2461 ggccagtctt gccgcacgtg ccgtcaaagc ctacgcccgt cgtgtcatca agaaagttcc
     2521 gcgcgatccg gaacatctgg tgcagcatct gcgccgggcg ctgaataatt ccccgctgcc
     2581 caactggctg ccgcgtggcg tgcggcggac ccagtttgcg gtagagggcg tgcggggtga
     2641 atggctgcgg gtgaacaatc cgacgcaggt catcctgtat ctgcatgggg gtggttatat
     2701 cgccggcgtg cccactacct atctgaccct gtgtgcaaaa tttgccaagg cattgcaggc
     2761 cgatgtgtat atgccggcct accgtctggc gccggaacac ccgttccccg ctgcactgga
     2821 cgatgcgatg acggtgtatc gcctgcttct gaaaaaa
//
LOCUS       DASUEG010000113         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_790445, whole genome shotgun sequence.
ACCESSION   DASUEG010000113 DASUEG010000000
VERSION     DASUEG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790445"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFX11_02010"
     CDS             <1..318
                     /locus_tag="VFX11_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937417.1"
                     /translation="QSNAGLRRCWWYTCLEENINPKNATRMPLLFALDAKLNIPNGFR
                     YTLDCSKMKSGGGDGSNTSVVHCGSDFSNSSFDGTTDGFGGESSGGGADGSGCGGGCG
                     GGD"
     gene            complement(487..1284)
                     /locus_tag="VFX11_02015"
     CDS             complement(487..1284)
                     /locus_tag="VFX11_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937418.1"
                     /translation="MAEFRDRYGPWALVTGAGNGLGAEFARQLAKQGLNVVLADLDAA
                     GLDAVATELRVQTGVEIRTVQLDLAQPAFMTQLDPVIQGLDIGLVINNAGISALGLFT
                     GRPLQDHLSVVDVNVRAPLVLAHHFAPRLIQRGKGGLLFVSSLSALQGTAYVANYAAT
                     KSWNLIFAEGLWEEMQGTGVDVLGYLVGSTRTPGFVNSRPRLERASLVKVMEMPAVVK
                     GALASLGKGPTCVAGAGNGMAAALLCRLLPRRWAVRVVSGTTRKLYG"
     gene            1359..2162
                     /locus_tag="VFX11_02020"
     CDS             1359..2162
                     /locus_tag="VFX11_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4937419.1"
                     /translation="MSPILTLNVHEILRAATGSIECTLDLGKTTSRVEVSADGWHVEG
                     QHYPFMAKCKDRTIYYWDGESFEPVSRYSGALIKLVPTEWGPPTFEIDGIKMLPSAQL
                     SPYDDARRKVDLIQPEGKTILDTCGGLGYFAAWCLQRNARQILSFEKNPDVIWIRSHN
                     PWSPVADGHYQLEQGDSAQQIPQLPAASFDAILHDPPRFGIAGELYSQAFYHQLARVL
                     KRKGRLFHYTGTPNKLTSGRDVPNEVAKRLREAGFTTEIIGDGVLAIKK"
     gene            complement(2204..>2690)
                     /locus_tag="VFX11_02025"
     CDS             complement(2204..>2690)
                     /locus_tag="VFX11_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2860 family protein"
                     /protein_id="HEX4937420.1"
                     /translation="KIEIDNEESGSALPLLTANERDMLDRNGATSQVNASYYWKLSPR
                     LFLIPAVIVGRNALDGSAVSCDSAGAKLDIGYQDGVNIVTVGLYAGQSTYDEGHPVFA
                     NTKADSSEFVGGVSYIRRNFFGQKHWASVVNLAYGDSDSDIDFFDTEIRRLAVGAMYI
                     F"
BASE COUNT          594 a          864 c          726 g          506 t
ORIGIN      
        1 cagagtaacg ccgggctcag acgttgctgg tggtatacct gcctggaaga aaacatcaat
       61 ccaaaaaatg ccacaaggat gccgttactg tttgcgctgg atgcgaaact gaatattccc
      121 aatggctttc gctatacgct cgactgcagc aaaatgaagt caggcggtgg cgacgggagc
      181 aacacctctg tcgtccattg tggaagcgat ttttcaaatt caagttttga tggcaccacg
      241 gacggctttg gcggtgaatc ctcgggtggc ggcgccgatg gcagtggctg cgggggcggt
      301 tgcggcggag gtgactgaag tgacaccccc taaccagccg gcaccgactg ttcccctgcc
      361 gggaactctg acagaatcgc cattccggat tcccgataac catcaggcca cccactatgc
      421 tcatcccgca agcctgaaaa ttcacgcccc ggtcgagacg atcagttggc gcaggctcga
      481 tgcccatcac ccatacagct tccgcgtagt gccggacacc actctcaccg cccatctgcg
      541 cggcaacagc cggcacagca acgccgccgc catgccgttg ccggcacccg ccacgcaggt
      601 aggccccttg cccagactgg ccagcgcgcc ctttaccacc gctggcattt ccatcacctt
      661 caccaatgac gcacgctcca gccgggggcg ggaattcaca aagcccggcg tgcgggtgga
      721 acccaccaga taacccagca catccacgcc ggtgccctgc atttcttccc acaaaccttc
      781 ggcgaaaatc aggttccagg atttggtggc ggcatagttg gccacatacg cggtaccctg
      841 caatgccgaa agggaggaaa cgaacagcag gccgcccttg ccccgctgga tcaaccgggg
      901 cgcgaagtga tgggccagca ccagtggcgc acgcacattc acatccacga cactcaggtg
      961 atcctgcaac ggccgcccgg tgaacagccc aagcgcgctg atccccgcat tgttgatcac
     1021 cagcccgata tcgagccctt gaatcaccgg gtccagctgc gtcatgaatg ccggttgcgc
     1081 cagatccagc tgcacggtgc gtatttcgac gcccgtctgc acacgcaatt ccgttgcgac
     1141 cgcgtccagg ccagcggcat ccagatccgc cagcactacg ttcaggccct gttttgccag
     1201 ttgccgggcg aattccgcgc ccaagccatt acccgcgccg gttaccagcg cccagggtcc
     1261 ataccgatcc ctgaattcag ccattaggat tttcctgtcg gtttcgatat aaaggatttg
     1321 atagtattgc ccaccccagc cagtgacgtg acaatcccat gagtcccatt ctcacactca
     1381 acgtacatga aatcctgcgc gccgcaaccg gcagcatcga gtgcacgctg gatctgggca
     1441 aaaccacgtc gcgggtcgag gtcagtgccg atggctggca tgtcgaaggc cagcactatc
     1501 cgttcatggc caaatgcaag gaccgcacga tctactactg ggatggggaa tcgttcgagc
     1561 cggtttcacg ctacagcggc gccctgatca agctggtacc gacggaatgg gggccaccca
     1621 cgttcgaaat cgacggcatc aagatgctgc ccagcgcgca gctgtcacct tacgacgacg
     1681 cgcgcagaaa ggtcgacctg atccagccgg aaggcaaaac cattctcgac acctgtggcg
     1741 ggctgggcta tttcgccgcc tggtgtctgc agcgcaatgc ccgccagatc ctgtccttcg
     1801 agaaaaaccc cgacgtgatc tggatacgca gccacaaccc ctggtcaccc gtggcggatg
     1861 gacattacca gctggagcag ggcgacagtg cgcagcagat tccccaactg ccggctgcgt
     1921 cattcgatgc gattctgcac gacccgcccc gcttcggcat tgccggcgaa ctgtattcgc
     1981 aggctttcta tcaccagctg gcgcgtgtcc tgaagcgcaa gggcaggctt tttcactata
     2041 cgggaacccc gaacaagctc acgagcgggc gcgatgttcc caatgaagtc gccaagcggt
     2101 tgcgggaagc aggatttacc accgagatca ttggcgacgg cgtgctggcg atcaagaaat
     2161 agcgggatgc cagcacggat tgcccggctg gatcagccca ccgtcagaaa atatacatgg
     2221 cacccaccgc caggcggcga atctcggtat caaagaaatc aatatcggaa tcggaatcgc
     2281 catacgccag attgacgacg ctggcccagt gcttttggcc aaagaaattg cggcggatat
     2341 aactcacacc ccctacgaat tcactggaat ccgctttcgt attggcaaaa accggatggc
     2401 cttcgtcgta ggtactctgg cctgcataaa gccccaccgt cacaatgttc accccatcct
     2461 gatagccgat atccagcttg gcgccagcac tgtcgcagga caccgctgaa ccgtccagcg
     2521 catttctgcc gacaatgacg gccggaatca ggaacaaccg cggcgacagt ttccagtaat
     2581 agctcgcatt cacctggctg gtggcgccgt tgcgatccag catgtcgcgc tcattggccg
     2641 tcagcaaagg caatgcgctg ccgctttcct cattgtctat ttcgattttc
//
LOCUS       DASUEG010000114         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2027457, whole genome shotgun sequence.
ACCESSION   DASUEG010000114 DASUEG010000000
VERSION     DASUEG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027457"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..2160)
                     /locus_tag="VFX11_02030"
     CDS             complement(121..2160)
                     /locus_tag="VFX11_02030"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEX4937421.1"
                     /translation="MTHPVFPNLFSPLDLGFTRLKNRVLMGSMHTMLEEMPNGFEKMA
                     AYYGERAAGGVGMIITGGIAPNEVGLPTPGLAKLSTPEEAAHHRIVTERVHAIAPDCK
                     ICMQILHVGRYAYHPNLVAPSPIKARISPCVPNELTEEQIWEQIGDFVNCALLAKEAG
                     YDGVEVIGSAGYLISEFLVTHTNRRTDAWGGPYENRMRFALEVITRIRQAVGPNFILV
                     YRIAAMELLENGSSWDEVVTLAKAIEKAGATIISTHFTWHEARVPTLATMVPRAAFTG
                     VTARLRKELTIPVITSNRINMPDVAEQVLAEGRADIVSLARPMLADPEFVIKARDNRV
                     DEINTCIACNQACLDHAFSGKLTTCLVNPRACYETELNFLPVTQVKKIAVVGAGPAGM
                     AFAINAAGRGHQVTLFESSDRIGGQFNIAKRIPGKEEFNETLRYYSRQIELTGVQLKL
                     NQRVTAQELIAGGFDEVVLATGVVPRTPDIAGIDHPMVMGYLDAILGRKKVGKAVAII
                     GAGGIGFDVAELISHKGVSSALSLEKFGAEWGVDFNNHPRGGVAGVEPQVEKADRDIW
                     LLQRKTTPVGAGLGRTTGWTHKILLERKDVRMMNGVEYQRIDDAGLHILVGNQPITLP
                     VDTVILCAGQEPLRELQADLEAANVKVHLIGGADVAAELDAKRAISQASWLAAAI"
     gene            complement(2281..2907)
                     /locus_tag="VFX11_02035"
     CDS             complement(2281..2907)
                     /locus_tag="VFX11_02035"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937422.1"
                     /translation="MAIDPNGLNTSGLKKTPTQPRARRTVERIIAATAALIEEQGLDA
                     LTTNRVAERANVNIASLYQYFPNKQALLSALLQQYWQDLARVLNDLLDQLGDVTVDES
                     TRLWAQLGIQHFRNQGGVLVELLRHPSQLAALPEGREFEHRLMEAMRRFLTRQRDRLN
                     VPDLDRAIYVAFQACTAILSRHLLEPMPYYRDDEIVEELARLMRGYFY"
     gene            2985..4088
                     /locus_tag="VFX11_02040"
     CDS             2985..4088
                     /locus_tag="VFX11_02040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4937423.1"
                     /translation="MSKATSPNPAWIEKYADSHMDRLRHTCDPLADAVAVRIDRKRPS
                     HMIDEVYARAKTEGGPFQAFVDHAHTVPGWVDWGRIEQARRVNLAFANLRATSLLVSS
                     LVEGYSLSKAAHVLVATGRLHQDVLKRIYETSQMSHNMNVPGGLRPGAHGHRTMMEVR
                     LLHAMVRKYLRGHGWDVTLYDEPINQEDMAFTIIEFDYLALRGMQRMGAELSQDDQDA
                     MHHLWRYAAYLNGVDDYLNTESLDEEIYLYQRIRDRQRNPNDESRLLAHSVITALAGQ
                     PPFNLPAPLLYELSRLCLGDELADAYLLPRNRLWQRALQLYRAGNRIGALAHYHVPGM
                     DRLSEKLNFRLLQKAIRENLDADPEKRAFRHIA"
     gene            complement(4121..4525)
                     /locus_tag="VFX11_02045"
     CDS             complement(4121..4525)
                     /locus_tag="VFX11_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016957623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4937424.1"
                     /translation="MQIVNQLDDAQTRELHRLYQNEWWSQGRTLEDTIRCVNGSSIVF
                     GFIEQGQLIAFARVVTDYVFKAFIFDVIVAQSARGKRLGERMIETIQAHPDLQRVKHI
                     ELYCRPDMVPFYQRYGFTTDVGDIILMRHTTC"
BASE COUNT          933 a         1408 c         1431 g          978 t
ORIGIN      
        1 gctgcaaacg cttttcggca tcggcatccc agatcggttt gtccttgctc atggggattg
       61 gggctcctgc tgcggtgtct ggctttaacc atagcacaga caccgccaca gcgtttttgt
      121 tcagatagcc gccgccagcc aggacgcctg actgattgcg cgcttggcgt ccagttccgc
      181 tgccacatca gcgccgccga tcaggtgcac tttcacgttt gccgcttcca gatccgcctg
      241 caattcccgc agtggctcct gtccggcgca cagaatcacg gtatccaccg gcagcgtgat
      301 gggctggttg cccaccagaa tatgcaatcc cgcgtcatca atgcgctggt attccacgcc
      361 gttcatcatg cgcacatcct tgcgctccag caggatcttg tgggtccagc cggtggtacg
      421 gcccaggccc gcgcccacgg gcgtggtctt gcgctgcagc agccagatgt cgcggtcggc
      481 cttttccacc tgcggttcca cgcctgccac gccgccgcgc ggatgattgt tgaaatccac
      541 gccccattcc gcgccgaatt tttccaggct cagggcggag gaaacgccct tgtgggaaat
      601 cagttccgcc acgtcgaatc cgatgccgcc agcgccgatg atcgccaccg ccttgccgac
      661 ttttttgcgg ccgagaatcg catccagata gcccatcacc atcggatgat cgatgccggc
      721 gatatcgggt gtgcgcggta ccacaccggt ggccagtacc acctcgtcga agccgccggc
      781 aatcagttcc tgtgccgtca cgcgctggtt cagtttcagc tgcaccccgg tgagttcaat
      841 ctgccggctg taatagcgca gggtttcgtt gaattcctcc ttgccgggaa tccgtttggc
      901 gatgttgaac tggccaccga tgcggtcgct gctttcaaac agtgtcacct gatggccgcg
      961 ccctgcggca ttgatcgcaa acgccatgcc tgcagggccg gcgcccacga cagcgatttt
     1021 tttaacttgg gtaaccggca aaaaattcag ttcggtttca tagcacgcgc gcggattcac
     1081 cagacaggtg gtgagcttgc cgctgaaggc gtgatccaga caggcctggt tgcaggcaat
     1141 gcaggtattg atttcatcga cgcggttgtc gcgcgcttta atcacaaatt caggatcggc
     1201 cagcatcggc cgtgccagcg acacaatgtc ggcgcggcct tccgccagca cctgttccgc
     1261 cacatcgggc atgttgatgc ggttgctggt gatcaccgga atagtcagtt ccttgcgcag
     1321 gcgcgcggta acgccggtaa atgcggcgcg cggcaccatg gtggccagtg tgggaacccg
     1381 cgcttcatgc caggtgaaat gcgtgctgat gatcgtggca ccggcttttt caatcgcttt
     1441 ggccagtgtt accacttcgt cccaggaact gccgttttcc agcaattcca tcgcggcgat
     1501 gcggtacacc agaatgaaat tcggccccac cgcctgacgg atgcgcgtaa tcacttccag
     1561 cgcaaagcgc atgcggtttt catacggccc gccccaggcg tcggtgcggc ggttggtgtg
     1621 ggtcaccaga aattcgctga tcaggtagcc ggcggagcca atcacttcca cgccgtcgta
     1681 acccgcttcc tttgccagca gcgcgcaatt cacgaagtcg ccaatctgtt cccagatctg
     1741 ttcttccgtc agttcattgg gcacgcacgg gctgatgcgc gccttgatcg gcgacggcgc
     1801 caccagattg ggatgatagg cataacggcc cacgtgcaga atctgcatgc agatcttgca
     1861 gtccggtgcg atggcgtgca cacgctcggt gacgatgcgg tgatgcgccg cttcctccgg
     1921 cgtcgacagt ttggccaggc ccggcgtggg caatcccact tcgttgggcg cgatgccgcc
     1981 ggtgatgatc atgccgacgc cgccggctgc gcgctcgccg tagtaggcgg ccattttttc
     2041 aaaaccgttg ggcatttctt ccagcatggt gtgcatggag cccatcagta cgcggttttt
     2101 caggcgggtg aagccgaggt ccagtgggga aaacaggttc gggaaaacag ggtgcgtcat
     2161 attgtgcgtt ccgggattcg atggcgaggg tcggttgcag ggatgggctg gattatggga
     2221 agggcggcaa cggtgcggca atgacggtaa ctgtcaaaaa ggtgaacgtt tggagcagca
     2281 tcaataaaaa tacccccgca tcagcctggc cagttcctcc acgatctcgt catcccggta
     2341 gtaaggcatg ggctccagca gatgccgcga cagaatggcg gtgcaggcct gaaacgccac
     2401 gtaaatggca cggtccagat ccggcacatt cagccggtcg cgctggcggg tcagaaagcg
     2461 ccgcatggct tccatcagcc ggtgttcgaa ttcgcggcct tccggcaacg ccgccagctg
     2521 gctgggatgg cgcagcagtt ccaccagcac accgccctgg ttgcgaaaat gctggatgcc
     2581 cagctgcgcc cacaggcggg tggattcgtc cacagtcaca tcgcccagct gatccagcag
     2641 atcgttcaac acccgcgcca gatcctgcca atactgctgc aacagcgcgc tcagcagggc
     2701 ctgcttgttg ggaaagtact ggtacagcga cgcgatgttc acattggccc gctctgccac
     2761 ccggttggtg gtgagggcat ccaggccctg ttcctcgatc agtgcggcgg tggcggcgat
     2821 gatgcgctcc acggtgcggc gggcgcgggg ttgggtgggc gtctttttca gaccgcttgt
     2881 attcaggccg ttggggtcga tagccataaa aaccaagttt tatgtaagta aatacttata
     2941 ttagagtctg gcatagcctt cccgacactg caaccggagt ccccatgtct aaagcaacct
     3001 cgccgaatcc cgcctggatc gaaaaatacg ccgactccca catggaccgc ctgcgccata
     3061 cctgcgatcc cctggcggac gctgtggcgg tgcgtattga tcgcaagcgg ccgtcgcaca
     3121 tgatcgacga ggtgtatgcc cgcgccaaaa ccgaaggcgg gccgttccag gcgtttgtcg
     3181 atcatgccca tacggtaccg ggctgggtgg actggggccg catcgagcag gcgcgacggg
     3241 tgaatctggc attcgccaac ctgcgtgcca cgtcgctgct ggtgagcagc ctggtggaag
     3301 gttattccct cagtaaggcg gcccatgtgc tggtggccac cgggcggctg caccaggatg
     3361 tgctcaagcg catttatgag acgtcgcaga tgagccacaa catgaatgta cccggtggac
     3421 tgcgccccgg agcgcatggc catcgcacca tgatggaagt gcggctgctg catgcgatgg
     3481 tacgcaaata tctgcgcggg catggctggg acgtgacgct gtatgacgag cccatcaacc
     3541 aggaagacat ggccttcacc atcatcgaat tcgattacct ggcgttgcgc ggcatgcagc
     3601 gcatgggcgc ggaactgagc caggacgatc aggacgccat gcaccatctg tggcgctacg
     3661 cggcctacct gaacggtgtg gacgattacc tgaacacgga atcactggac gaggaaattt
     3721 acctgtacca gcgcatccgc gatcgccagc gcaatcccaa cgatgaaagc cgcctgctgg
     3781 cacattcggt gatcacggcg ctggcggggc agccgccatt caacctgccg gcgccgttgc
     3841 tgtatgaatt gagccgcctg tgtctgggcg atgaactggc ggacgcgtat ctgttgccgc
     3901 gcaaccggtt gtggcagcgg gcgttgcagt tgtaccgcgc cggcaatcgc atcggcgcac
     3961 tggcgcatta tcacgtaccc ggcatggatc gtctgagcga gaaactgaat ttccgactgc
     4021 tgcagaaagc gatacgggaa aatctggatg ccgatccgga aaagcgggcg ttccggcata
     4081 tcgcataatg gtgtgcagtc attttggcag ggcagttttg ctagcaggtt gtatgacgca
     4141 tcagaatgat atccccgacg tcggtggtaa atccgtaacg ctggtagaac ggcaccatgt
     4201 ctggccggca atacagttca atgtgcttga cgcgctgaag atcgggatgc gcctgtatgg
     4261 tttcaatcat gcgctcgccc agccttttgc cgcgggcgga ctgtgccacg atcacatcga
     4321 aaataaaggc cttgaacaca tagtccgtca ccacccgcgc gaacgcgatc agctggccct
     4381 gctcgataaa accaaaaaca attgaagagc cgttgacgca gcggatcgtg tcctccagcg
     4441 tgcggccctg cgaccaccat tcgttctggt acagccggtg cagttcgcgt gtctgcgcgt
     4501 cgtcgagttg attcacaatc tgcatgtcga tgccctcatg cgccgacggg agtgggaatg
     4561 ggaaaattgg gatggcaggc gtttcagatt tttctcgcgg tcacgaatac tgccagatag
     4621 gcctggccgt tgtcgaaaat gaacacccgg aaatccgatg caaaaaaggt gtacagatac
     4681 ggcagatcca gcgcaccaat gaaggcatcg gcgtcgatca gccgcagcag cttgcggcca
     4741 tagttgatca
//
LOCUS       DASUEG010000115         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1960834, whole genome shotgun sequence.
ACCESSION   DASUEG010000115 DASUEG010000000
VERSION     DASUEG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960834"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="atpA"
                     /locus_tag="VFX11_02050"
     CDS             complement(<1..992)
                     /gene="atpA"
                     /locus_tag="VFX11_02050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4937425.1"
                     /translation="MQQLNPSEISELIKQRIANLDTTSEARNEGTIVSVSDGIVRIHG
                     LADVMYGEMIEFQGGVFGMALNLEQDSVGAVVLGEYLGLAEGQKVKCTGKVLEVPVGP
                     ELLGRVVDSLGNPIDGKGPLNTKLSDVVEKVAPGVIARKSVDQPVQTGLKNIDSMTPI
                     GRGQRELIIGDRQIGKTAVAIDTIINQKGTGIKCIYVAIGQKQSSIANVVRKLEEHGA
                     MDHTIVVAAAAADPAAMQYIAAFTGCTMGEYFRDRGEDALIVYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSAEEGGGSLTALPVIETQANDVSAY"
     gene            complement(1009..1545)
                     /locus_tag="VFX11_02055"
     CDS             complement(1009..1545)
                     /locus_tag="VFX11_02055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX4937426.1"
                     /translation="MAELTTIARPYAKAAFQFADESGALTQWSAMLGFAAAVAGDASV
                     SRLLANPKVTAEQKADGFLKLCADKLDPAGRNFVSLLAHNKRLAALPAIAVLFEELKS
                     QKEQSVDVEIVSAFALSADQSSKLAASLKKRLGREVNVTTAVDPSILGGAIIKAGDLV
                     IDGSVQGKLARLAETLNS"
     gene            complement(1557..2027)
                     /locus_tag="VFX11_02060"
     CDS             complement(1557..2027)
                     /locus_tag="VFX11_02060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX4937427.1"
                     /translation="MDINATILGQVVAFAIFVWFCMKFVWPPLIKAMHERQEKIAHGL
                     QAADQAGKDLELAKEKAAETLREAKHQAAALIEQANKRANQLVEEAKEQARVEGERLK
                     HSAQTEIEREVNRAKDQLRAQVAALSIAGAEKILAASVDQAAHGKMLEQLAAEL"
     gene            complement(2086..2322)
                     /gene="atpE"
                     /locus_tag="VFX11_02065"
     CDS             complement(2086..2322)
                     /gene="atpE"
                     /locus_tag="VFX11_02065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX4937428.1"
                     /translation="METASALVLIAAGLVIGLGALGTAIGFGLLGGRLLEGTARQPEM
                     APMLQGKMFLMAGLLDAVPMIGVGIGMYMIFVLA"
     gene            complement(2377..3213)
                     /gene="atpB"
                     /locus_tag="VFX11_02070"
     CDS             complement(2377..3213)
                     /gene="atpB"
                     /locus_tag="VFX11_02070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX4937429.1"
                     /translation="MAAGSASDYIKHHLTNLVFGQKADGTWGLAHDAAEAAQMGFWKF
                     HVDSLGWSFGLGILFLFLFSRAAKRATTGVPTGFQNFVEIMVDFVSTQVKENFHGRNP
                     LIAPLALTIFCWVFLMNLMDLIPVDWIPSLFALFGVHYMKIVPSTDPNITLGMSFTVF
                     AMIIYYSIKVKGIGGFAGELTLHPFSSDNIAVKILLIPINLFLETINLIAKPISLGLR
                     LFGNMYAGEMIFILIALLPWFLQWTLSVPWAIFHILVITLQAFLFMVLTIVYLSTAHE
                     DH"
     gene            complement(3260..3655)
                     /locus_tag="VFX11_02075"
     CDS             complement(3260..3655)
                     /locus_tag="VFX11_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX4937430.1"
                     /translation="MTKPRAINDRHLAYYVVGTQAAVSVLLPVLVWIVLGNGAAVAAL
                     AGGWIATLGNLYFAIQAFRFSGARASRDMVRAFYRGEAGKFVIVMLLFIATFRLVDGI
                     RDTAPYLFAAFFLVYGIAWMAPLILRRGK"
     gene            complement(3830..4723)
                     /locus_tag="VFX11_02080"
     CDS             complement(3830..4723)
                     /locus_tag="VFX11_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX4937431.1"
                     /translation="MSAKKKGLGRGLDALLASASLPKKEVPTDPAQPVPASENLDGEL
                     RHLPIEFLQRGRYQPRRDMDPQALEDLAASIKAQGVMQPIVVRPIGGKNYEIIAGERR
                     WRASQLASLEKVPCLIRDVPDEAAIAMALIENIQREDLNPIEEAAALQRLQDEFELTH
                     QQVADAVGKSRTTVTNLLRLMSLSAEARKLVEHGDLEMGHARAILTLDTLKQTEAART
                     IAAKGLSVRQTEALVRRLQQEQIASGDVRLDPDIKLLQDNLSDKLGAPVLIQHSAKGK
                     GRLVIRYSSLDELDGILDHIK"
     gene            complement(4797..5597)
                     /locus_tag="VFX11_02085"
     CDS             complement(4797..5597)
                     /locus_tag="VFX11_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4937432.1"
                     /translation="MGKVLAVTNQKGGVGKTTTSVNLAASLVATKRKVLLIDLDPQGN
                     ATMGSGVNKHALENSIYDVLVDELSIAQARVTTSPGFDLVAANGDLTAAEVELLDMDD
                     KEQRLKRALAPVQGEYDYIIIDCPPSLNMLTVNALVASDSVLIPMQCEYYALEGLTAL
                     LNTIEKIKAVLNPQLEIDGLLRTMYDPRIALANQVSSQLTDHFGDKVYRTIIPRNVRL
                     AEAPSHGLPVLEYDAKSRGAVCYLALAGELIRRAEQKKRDPAVETLSA"
     gene            complement(5609..6265)
                     /gene="rsmG"
                     /locus_tag="VFX11_02090"
     CDS             complement(5609..6265)
                     /gene="rsmG"
                     /locus_tag="VFX11_02090"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX4937433.1"
                     /translation="MAVTNQQRELLAQGCGELGLALSEQQQQTLNAYLDSLVKWNQAY
                     NLTAIRDPGEMVVKHLLDSLAIHRAVNGNSILDVGTGPGLPGIPMAICRPHQHWTLLD
                     SNGKKTRFLLQMKHELRLDNLSVIKDRIEALPQQQQFDCITCRAFSSLQDFVGHCLRL
                     LKPGGYLLALKGQIPAEEIAALDQNTLDIAIEKIAVPFLHEERHLINIRKKNAGSQIS
                     "
     gene            complement(6277..>6800)
                     /gene="gidA"
                     /locus_tag="VFX11_02095"
     CDS             complement(6277..>6800)
                     /gene="gidA"
                     /locus_tag="VFX11_02095"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX4937434.1"
                     /translation="GMVCDARWEAFCEKREAIVREEQRLRSTWILPQSELAQKLNPQL
                     DNPLSHEYSLMDLLRRPELSYAQLAELSNSEPVAPVVAEQIEIAAKYSGYIVRQQEEV
                     EKLRRSEETRVPADFDYDAVKSLSHEVRNKLKQSRPETLGQASRIPGVTPAAISLLLI
                     HLKKRNGQQQKSA"
BASE COUNT         1333 a         1969 c         2017 g         1481 t
ORIGIN      
        1 atataggcag agacgtcgtt ggcctgggtc tcaatgaccg gcaacgctgt caaactgccg
       61 ccgccttctt cagccgacaa cttggcggcc cgttccaaca aacggctatg gagatagaaa
      121 acgtcacccg gatacgcttc acggcccggc ggacggcgca gcaacagcga aatctggcgg
      181 taagcccagg cctgtttggt caagtcatca tatacgatca gggcgtcttc accgcggtca
      241 cggaaatatt cgcccatggt gcagccggtg aaggcggcga tgtactgcat cgcggccgga
      301 tcagcagcgg cagcggccac tacgatggtg tggtccatcg cgccgtgctc ttccagcttg
      361 cgcacgacgt tggcgatgga ggactgcttc tgtccgatcg ccacgtagat acacttaata
      421 ccggtgcctt tctggttgat gatggtgtcg atcgccacgg cagtcttgcc gatctggcgg
      481 tcgccgatga tcagctcgcg ctggccgcgg ccgattggcg tcatggagtc gatgttcttc
      541 agaccggtct gtaccggctg gtccaccgat ttccgcgcga ttacacccgg tgcaaccttt
      601 tccaccacgt cagacagctt ggtgttgagc gggcctttgc catcaatagg gttacccagg
      661 gaatccacca cgcggcccag cagttccgga ccgaccggca cttccagtac cttgccggta
      721 catttcactt tctggccttc tgccagaccc aggtattcac ccagtaccac cgcgcccacg
      781 gaatcctgtt ccaggttcag cgccatgccg aacacgccgc cctggaattc gatcatctcg
      841 ccgtacataa catcggccag accgtggatg cgcacaatac cgtcagacac gctgacgatg
      901 gtaccttcgt tgcgggcttc ggacgtggtg tccagattcg cgatccgctg tttgatcagc
      961 tcgctgatct ctgacgggtt gagctgctgc atggttagat cctcaaactc aagaattcaa
     1021 tgtttcggcc agtcgggcca gcttgccctg cacggatcca tcaatcacaa ggtcacctgc
     1081 cttgatgatg gcgccaccca gaatcgaagg atcgacagcg gtggtgacgt tgacttcccg
     1141 gccgaggcgt ttcttcaggg aagcagccag tttgctggac tggtcggcgc tcagggcaaa
     1201 tgcagaaacg atttccacat cgacgctttg ttccttttgc gatttcagct cttcgaacag
     1261 caccgcgatc gccggcaggg ccgccagtcg cttgttgtgg gccagcaggg acacaaaatt
     1321 gcgtccggcc gggtccagct tgtccgcgca cagtttcaga aatccgtccg ccttttgctc
     1381 ggctgtaacc ttcgggttgg ccagcaatcg ggaaacgctg gcgtcacccg caacagcagc
     1441 ggcgaagccc agcatcgccg accattgtgt cagggcgccg gattcgtcag cgaactgaaa
     1501 ggctgctttc gcgtagggtc gggcaatcgt tgtaagttct gccatatccg ttaccgttac
     1561 agttcagctg caagctgctc aagcatcttg ccgtgggcgg cctgatcaac cgaggcagcc
     1621 aggatttttt ccgcgccggc gatggacaga gccgccacct gcgcacgcaa ctgatccttg
     1681 gcccggttca cttcgcgttc gatttcagtc tgtgcagaat gtttcaggcg ctcaccctcg
     1741 acacgggcct gctccttggc ttcttccacc agctgattgg cgcgcttgtt ggcctgttcg
     1801 atcagggcag cggcctggtg cttggcttcc cgcagggttt ccgcggcttt ttctttagcc
     1861 agttccagat ccttgccagc ctggtcggcc gcctgcagac cgtgggcgat cttttcctgc
     1921 cgttcgtgca tggccttgat caatggcggc cacacgaatt tcatgcagaa ccatacgaag
     1981 atggcaaagg cgacgacctg tcccaggatg gtagcgttaa tatccatacc aaaccctctc
     2041 gctcgttaaa gtaaaaagaa tgcgttcttt tttctgagcg aagacttagg ccagaacgaa
     2101 gatcatgtac atgccgatac caacgccgat catcggcacc gcgtcgagca gacctgccat
     2161 caggaacatt ttgccttgca gcatcggagc catttccggt tgacgtgcgg taccttccag
     2221 caggcggcca cccagcaggc cgaagccgat tgcggtgccc agcgcgccca gaccgatgac
     2281 cagaccagcg gcgatcaata caagtgcaga tgcagtttcc atgttatctc ctggtgtttg
     2341 acttatttaa aggtgaatga aaaagcggtt acgttatcag tgatcttcgt gcgccgtgct
     2401 caggtacacg atggtcagga ccatgaacag gaacgcctgc agggtaatga ccagaatgtg
     2461 gaaaatcgcc cagggtaccg acagggtcca ctgcaggaac cagggcagca gcgcgatcag
     2521 gatgaagatc atttcgccgg cgtacatgtt tccgaacaga cgcaggccga gggaaatggg
     2581 cttggcaatc aggttgatgg tttcgaggaa caggttgatc ggaatcagca gtatcttgac
     2641 ggcgatgttg tcgctgctga acgggtgcag ggtcagttcg ccggcaaaac cgccgatgcc
     2701 tttcaccttg atgctgtagt agatgatcat ggcgaacacg gtgaaggaca tgcccagggt
     2761 aatgttgggg tccgtggacg gcacgatctt catgtaatgc acgccaaaca gggcgaacag
     2821 ggagggaatc cagtccaccg gaatcaggtc catcaggttc atcaggaaca cccagcagaa
     2881 aatggtcaga gccaggggcg cgatcagcgg gttgcggccg tggaagtttt ccttcacctg
     2941 ggtgctgacg aaatccacca tgatctcgac gaaattctgg aagccggtgg ggacgccggt
     3001 ggtggcgcgc ttggccgccc tgctgaacag gaacaggaac aggatgccaa ggccaaatga
     3061 ccagccgagg gaatcaacgt ggaatttcca gaaacccatc tgggcggcct cggccgcatc
     3121 atgggccaaa ccccaggtac cgtcggcttt ctggccaaat acgagattgg tcaaatggtg
     3181 cttgatatag tctgaagcac ttccggctgc catagattat cccaataccc ttaacaaaaa
     3241 tcgcaggttt aagtcggcgt tacttgcctc gcctcaggat cagcggcgcc atccaggcga
     3301 tgccatagac cagaaaaaag gctgcaaaca gatacggtgc tgtgtcgcga atgccgtcta
     3361 cgagacgaaa ggtggcgatg aacagcagca tgacgatgac aaatttcccc gcctcgcccc
     3421 tgtaaaatgc ccgcaccatg tcgcgggacg cccgcgcgcc agagaatcgg aaagcctgta
     3481 tagcgaagta gagattgccc agcgtggcaa tccatccccc agccagcgcc gcaacggcag
     3541 cgccatttcc caagacaatc cacaccaaaa cagggagcaa aacagagacg gcggcttggg
     3601 ttcctaccac gtaatacgcg agatgacgat cgttgatcgc acgaggtttt gtcaaagcca
     3661 ttgttccaga taacccgaca ttcagcagag atccgtacct tttgggccag ccgatcgctg
     3721 agtgcaggtt cgagcctgcg taaggcggag ccgattatag agactttccc aggggcaggc
     3781 aacacgcggt ccgcgcggtt ttcgcgagtt tcggagggaa catgggccgc tattttatgt
     3841 ggtcaagaat accgtccagt tcatccaggc tggaatagcg gatgaccagc cggcccttgc
     3901 ctttggcact gtgctggatc agcaccggcg cacccagctt gtcggagaga ttgtcctgca
     3961 gcagcttgat gtccggatcg aggcggacat cgccactggc gatctgctcc tgttgcagac
     4021 ggcgcaccag ggcttcggtc tggcggaccg acagtccctt ggcagcgatg gtgcgggcgg
     4081 cttccgtctg cttgagggtg tccagggtaa gaatggcgcg ggcgtggccc atttccagat
     4141 cgccgtgctc caccagcttg cgggcctcgg cagacaggct catcaggcgc agcaggttgg
     4201 tgaccgtggt gcgggacttg cccacggcgt cggcgacctg ctggtgggtc agttcgaatt
     4261 cgtcctgcag gcgctgcagg gccgcggcct cctcaatcgg gttgagatct tcacgctgga
     4321 tgttttcgat gagggccatg gcgatggccg cttcgtccgg cacgtcgcga atcaggcagg
     4381 gcactttttc cagactggcc agctggctgg cgcgccagcg ccgctcaccg gcaatgattt
     4441 cataattctt gccaccgatc gggcgcacca cgatgggctg catcacgccc tgggccttga
     4501 tcgacgcagc gagatcttcc agggcttgcg ggtccatgtc gcggcggggc tggtaacggc
     4561 cgcgctggag gaattcaatc ggcaggtggc gcaattcgcc gtcgaggttt tcgctggcgg
     4621 gtaccggttg ggcaggatcg gtcggcactt cttttttcgg cagggaggca ctggccagca
     4681 gtgcgtcgag tccgcgacct aaacctttct tcttggcact catggttcgc ctttattgtc
     4741 gtgtgggggc cggctggggg agagccccat tataagcgcc ccctgtagcg gcgcaatcag
     4801 gcggacaggg tttccacggc aggatcgcgc tttttctgtt cagcacgacg gatcagttcg
     4861 ccggccagcg ccagatagca gacggcgcca cgggatttgg catcgtattc cagcaccggc
     4921 aggccgtggc tgggagcttc ggccaggcgc acgttgcggg gaataatggt gcggtagacc
     4981 ttgtcgccga agtgatcagt gagctggctc gacacctggt tggccagggc gatgcgcggg
     5041 tcgtacatgg tgcgcagcag accatcgatt tccagctgcg gattgagcac ggccttgatc
     5101 ttctcgatgg tgttgagcag cgcggtgagt ccttccagcg cgtagtattc gcactgcatg
     5161 ggaatcagca cggaatcgga tgccaccagt gcgttcacgg tgagcatgtt caacgacggc
     5221 gggcagtcga tgatgatgta gtcgtattca ccctgcaccg gcgccagtgc gcgtttcagg
     5281 cgctgttcct tgtcgtccat gtccagcagt tccacttcgg cagcggtgag atcgccgttg
     5341 gcggccacca ggtcaaaacc gggcgaagta gtcacgcggg cctgcgcaat ggacagctcg
     5401 tccaccagca cgtcgtaaat ggaattttcc agtgcgtgct tgttgacgcc actgcccatg
     5461 gtggcgttgc cctgcggatc gagatcgatc agcagcacct tgcgtttggt cgccaccagc
     5521 gaagccgcca aattcaccga cgtggtggtt ttgcccacgc cgcctttctg gttggtcact
     5581 gccagtacct tgcccaccac cttgctcctc aggatatctg tgatcctgca tttttcttgc
     5641 gaatgttgat caggtgccgt tcctcgtgca gaaagggcac ggcgattttt tcaattgcga
     5701 tgtcgagtgt gttctggtcc agtgccgcga tttcctcggc gggaatctgc cccttcagtg
     5761 ccagaagata accaccgggc ttgagcagac gcaagcagtg tccgacgaaa tcctgcagcg
     5821 atgagaaagc gcggcaggtg atacagtcga attgctgctg ttgcggcagt gcttcaatgc
     5881 gatccttgat gacgctcagg ttgtccagcc gcagctcgtg cttcatctgc agcagaaacc
     5941 gggttttctt gccgttactg tcgagcaggg tccagtgctg gtgtggccgg cagatcgcca
     6001 tgggtatccc cggcaggccg gggccggtgc ccacatccag tatggagttg ccgtttactg
     6061 cgcgatggat ggccagactg tccagcagat gcttcaccac catttcaccc ggatcgcgga
     6121 tggcagtgag attataggcc tgattccatt tcaccaggct gtcgagatag gcattgagtg
     6181 tttgttgctg ctgttcactc aatgccagcc ccagttcccc gcagccttgc gccagcaatt
     6241 cgcgctgttg attcgttact gccaatgccg tctcgctcag gctgatttct gttgttgtcc
     6301 gtttcgcttt ttaagatgaa tcagcagcag ggaaatcgcc gccggtgtga caccgggaat
     6361 gcggctggcc tggcccaggg tttccggccg gctctgcttc agcttgttgc gcacttcgtg
     6421 ggacaggctt ttcaccgcgt cgtaatcaaa atccgccggt acgcgggttt cttcgctgcg
     6481 acgcagtttt tccacttctt cctgctggcg tacgatgtag ccggaatact tggcggcgat
     6541 ttcaatctgc tcggccacca ccggcgcaac cggttcgctg ttgctcaatt ccgccagctg
     6601 cgcgtaactc aactccggcc ggcgcagcag atccatcaag gaatattcgt gcgacagcgg
     6661 attgtccagc tgcggattga gtttttgcgc cagttccgat tgcggcagaa tccaggtgct
     6721 gcgcaggcgc tgttcctcgc gcacgatggc ttcgcgtttt tcgcagaagg cttcccagcg
     6781 cgcgtcgcac accatgccca
//
LOCUS       DASUEG010000116         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1762151, whole genome shotgun sequence.
ACCESSION   DASUEG010000116 DASUEG010000000
VERSION     DASUEG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762151"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..969)
                     /locus_tag="VFX11_02100"
     CDS             complement(130..969)
                     /locus_tag="VFX11_02100"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4937435.1"
                     /translation="MSAVIQEKLKATEVSPFIGSRVHLSKDELLSGEFAAGLRELLEQ
                     RGVLIFPKINFTDEEQVRFTKTLGTLAPEMAGELLYKVTLDTSANSRADYLKGSWYWH
                     LDGTMNEVPILASILSARVLSPTGGNTEFCNTYSAWDQLPEEDKKHLEGLRVMHSAWT
                     SLFYYDPEPSLAKAQEMMRIGERVLPMVWKHRSGRKSLVLGCTAHHVEGMSARESTEL
                     LVRLREWATQPQFSYSHKWSVGDTVIWDNTGTMHRATPYDPASGRLMMRTKLQGEEPF
                     AYA"
     gene            complement(1032..1664)
                     /locus_tag="VFX11_02105"
     CDS             complement(1032..1664)
                     /locus_tag="VFX11_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4937436.1"
                     /translation="MTNKSTPRIAPLLPSDWDATAHDAVSAFPAARDFVLNNWENGGA
                     RGMNGLGAMLNHPSLAKAFLTFNNHVAATNTLSKRICELLILRISWLRRSEYEFQQHV
                     ILGLRAGLTAEEIDRVQEGPDAAGWNEIDTLLLRAADDLIADASVHHDTWSQLGQHFS
                     TEQMIDIVFTVGCYEVLAMAFKSFGVPFESSLDPMSPDVRARMNNSSWAK"
BASE COUNT          448 a          538 c          605 g          534 t
ORIGIN      
        1 ggtgtcgttg cagcgcgaga acctctgatt ggcttcaccg gttccatcct gtatgcaggc
       61 gcggaaccgg tgaagcaggc tggtgatttc gatcgaagtg cggatctgaa tttccgggct
      121 tgatatggat tacgcgtacg cgaaaggctc ttcaccctgc agtttggtgc gcatcatcaa
      181 acgtccggac gccgggtcat agggagtggc ccggtgcatg gttcccgtgt tgtcccagat
      241 cacagtgtca cccacggacc atttgtggct gtaggagaat tgtggctggg ttgcccattc
      301 acgcaggcga accagcagct cggtactctc gcgggcgctc atgccttcca cgtgatgtgc
      361 agtgcagccc agtaccaggg atttacgtcc ggagcgatgc ttccacacca tcggcaatac
      421 gcgttcacca attctcatca tctcctgtgc cttggccaga ctgggttccg gatcgtagta
      481 gaacagcgag gtccaggcgg agtgcattac acgaagacct tccagatgct ttttatcttc
      541 ttccggcagc tggtcccagg cggaataggt gttgcagaat tcggtattgc ctccggtggg
      601 ggacagcact ctggcagaga gaatggatgc caggatcgga acttcgttca tggtaccgtc
      661 gagatgccag taccaggagc ctttcaggta gtctgcccgt gagttggcgc tggtatccag
      721 ggttaccttg taaagcaatt cccccgccat ttcgggcgcc agtgtgccga gcgttttggt
      781 aaaacgtacc tgctcttcgt ctgtgaaatt gatttttgga aaaatgagca cgccccgctg
      841 ctcgagcagt tcgcgcaggc cggcagcaaa ttctccactc agcagttcat ctttgctcag
      901 atgaaccctg ctaccgatga aagggctgac ttcggttgcc ttcagttttt cctggataac
      961 ggctgacata atgtgatcct tctgaaaatt tgactatgac tggtctttga gcaaggttta
     1021 ggttgattta cttatttggc ccaggatgag ttgttcatcc tggctcggac atcgggtgac
     1081 attggatcca ggctggattc aaagggcaca ccaaaagact tgaacgccat tgccaacacc
     1141 tcatagcagc ccaccgtgaa tacgatgtcg atcatttgtt cggtactgaa gtgttggcca
     1201 agctggctcc aggtatcgtg atggacgctg gcatctgcaa tcagatcgtc cgcagcccgc
     1261 agcaatagcg tgtcaatttc gttccatccg gcggcatccg ggccttcctg tactcggtct
     1321 atttcttctg cggtcagtcc ggcgcgcagg cccagaatga cgtgctgctg aaattcatat
     1381 tccgaacgcc gcagccagct gatgcgcagg atcagcagtt cgcagatgcg tttcgacagg
     1441 gtgttggtgg cggcaacatg attgttgaag gtcaaaaagg cttttgccag ggatgggtgg
     1501 ttgagcatgg cgccgagacc gttcatgccg cgggctccgc cattttccca attgttgagc
     1561 acaaaatcgc gggcagctgg aaacgcgctc actgcatcat gagcggtagc atcccaatcg
     1621 gatggtaaca ggggcgcgat acgcggcgtg gatttgttcg tcatgttcag tgctctgctg
     1681 gataaccctt tatgcaatac agcaacgttg caggtcggga taagcgttac cggttttgaa
     1741 tattagtacc acacggtact aatagagtac agccggccgt gcagcatttc aaccgcaata
     1801 atttctgtta ttcaacttgt tgaatttcat ggctgacaga aggttgtcgt ggccagccat
     1861 aacgggtacg gactcatctt gatgagcggg caggtttgtc ttggtgtgga tgatcggcgc
     1921 ggacagtcag gatcggcaaa gcagaggggg gccacaccca agatgtgagt gagatatgaa
     1981 ttgagcgccc cttccggaca aagaagcgcg tattggaacc ggcctgtttc aatccttcct
     2041 gggaatgagt gtagtgaggt caaccgccag atctgccagg tcggtttcct tcatcactat
     2101 tttctggctg atgattttct tcgcc
//
LOCUS       DASUEG010000117         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_657831, whole genome shotgun sequence.
ACCESSION   DASUEG010000117 DASUEG010000000
VERSION     DASUEG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657831"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFX11_02110"
     CDS             <1..255
                     /locus_tag="VFX11_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX4937437.1"
                     /translation="MNRRVDGMYVALESRLGEAAYLGGAEFSCADIMSAFNLTSLTGL
                     GARVVDDSLPNTKAYLQRIVSRPAYQKAMAIAGPRASKPL"
     gene            complement(298..1125)
                     /locus_tag="VFX11_02115"
     CDS             complement(298..1125)
                     /locus_tag="VFX11_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4937438.1"
                     /translation="MRSLLFIVLLALVPLQLSAHGLVHDQISKLNHEIHHHPDKADLY
                     LQRGRLYLEEAHDPEAQRDFERALQLDRNARAAHYFLGQLALKRDEPDLALRHAETFI
                     ASLHGERGGLTRGYRLLGEIYSHQHEFGRAADAYRAAIAQAESPQPEYFLDLADACLK
                     QGAGHLDDAIAALDDGIRRLGPLTVLQDKAVEIESRAGRFDAALQRLDTMIAQGQRLP
                     ELYARQGRLLLAAGRTDAARESFQLALARLDSLPVARRETAAMRELRGSLQAQLSGT"
     gene            complement(1192..3333)
                     /locus_tag="VFX11_02120"
     CDS             complement(1192..3333)
                     /locus_tag="VFX11_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX4937439.1"
                     /translation="MTTFNSRVKYAALYGAVLTCALAQGAALAATVVRGPYLQMGDSD
                     SMVVRWRTDVATNSRVQFGTSQGSLAFSATNSTVTTEHEVAVTGLNPLTRYFYNVGDS
                     ASVHAGDASYYFETSPTPGAAVPTRIWVIGDAGTGSAGQAAVYNAYRNFTGTRYTDLW
                     LMLGDNAYNSGTDAEYQSKLFAMYPELLRQSPVWATLGNHDGASADSASQTGPYYNIH
                     TFPRNGEVGGVASGTEAYYSFDYGNVHFVVLDSYETSRSTSGAMMNWLQADLANNTAE
                     WLVAVWHHPPYSKGSHNSDTESNLIDMRQNFLPVLENYGVDLVLTGHSHSYERSKFID
                     GHYGLSSTYNSSYEIDGGSGRADGSGAYVKDASQPHSGAVYSVVGASGQTSGGTLNHP
                     AMYLSLNELGSMVIDVNGATMDLKYLNNNGTVRDYFTITKDSTPPAPPAAPSSLAATA
                     TSSSNINLTWTDNASDETGFDIERSLDGANWTALASVGSNVVSYSNGGLAASTTYFYR
                     VRARNGGGASGYSNVSSATTQAAGSNPVTVTLRDGLNSYAGTLDTYVASGKTGNNYGT
                     TAAVLADGADGSNGELVALLKWSVASIPASASVTNATVTLRVSNASAGTYRLYARNAS
                     WSEGSATWANADIAGSQGTEIGSFTPTATGSHTLTLNAAGIALVQGWVNGSLANNGFT
                     IRSGGTSDGVDMRSSEYGTASQRPALTVTYQ"
     gene            complement(3519..4040)
                     /locus_tag="VFX11_02125"
     CDS             complement(3519..4040)
                     /locus_tag="VFX11_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937440.1"
                     /translation="MNAYMMAAAAMVITKVSYDLLNDIRIRDRVYTQPLKTMPANAKQ
                     LIDEMMAEAAKDKSLSTDQLAQIEKGIYKMLSEGAVEQRNFEMKKTREMISYVGLFLS
                     WFMKQKGLSYEQIQAKYIKEFSVSEREGHLKIIESAAGSNSDLMGKFYITLRKKFGFG
                     ASASNSHPKQAKL"
     gene            complement(4232..>4495)
                     /locus_tag="VFX11_02130"
     CDS             complement(4232..>4495)
                     /locus_tag="VFX11_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HEX4937441.1"
                     /translation="YLRWLDKYDRAPGEESPIGLILCAGANAEQVELMDLSSANIRVA
                     EYLDHMPDMKVMQAQLHRAAELARERAARGRLPHVAETKGEPQ"
BASE COUNT          851 a         1334 c         1359 g          951 t
ORIGIN      
        1 atgaaccgtc gcgtggatgg tatgtacgtt gcactggaga gtcgcctggg ggaagcagcg
       61 tatctgggtg gggccgaatt cagttgcgca gacatcatgt cggcattcaa tctgacatcg
      121 ctgacgggtc ttggtgcgcg cgtggtggat gacagcctgc cgaataccaa agcctatctg
      181 caacgcattg tgtcaaggcc agcctatcag aaggccatgg ccattgccgg gccgagagca
      241 agcaagccgc tgtaggaaaa gcacctcgca cgctgcgttg gcctaacccg gcagcggtca
      301 cgttcccgac aattgcgcct ggaggctgcc gcgcagttcc cgcatggctg cggtttcgcg
      361 gcgggccacc ggcaggctgt ccagccgcgc cagtgccagt tggaaggatt cccgcgccgc
      421 atcggtccgc cccgccgcca gcagcaaccg gccctggcgc gcatacagct ccggcaggcg
      481 ctgcccttgg gcaatcatgg tgtccagacg ctgcagggcc gcatcgaacc ggccagcgcg
      541 ggattcaatc tccaccgcct tgtcctgcaa cacagtcagc ggacccagcc gccggatgcc
      601 atcgtccagg gcggcaatgg cgtcatcaag gtggcccgcg ccctgcttca ggcaggcatc
      661 cgccagatcc agaaaatatt caggctgcgg cgactcggct tgtgcaatgg cagcgcgata
      721 ggcatccgcc gcccgaccga actcgtgctg gtggctgtaa atttcaccca gcagccggta
      781 tccacgggtc aggccgccgc gctcaccgtg cagggatgca ataaaggttt cggcgtggcg
      841 caatgcaaga tccggctcat cgcgtttcaa tgccagctgt cccagaaagt aatgcgccgc
      901 gcgggcgttg cggtccagct gcagggcgcg ctcgaaatcc cgctgcgctt cagggtcgtg
      961 cgcttcttcc agatacagac ggccccgctg cagatacaga tcggccttgt ctggatgatg
     1021 atgaatctcg tgattcagct tgctgatctg gtcatgcacc aggccatgag cggacagctg
     1081 cagtggcacc agcgccagca gcacgatgaa aagcagtgat cgcatgctga tcctccgtca
     1141 aaacaaaagc cgggcgaaag cgcgtttcgc ccggcgtggt ggccaccgtt tttactggta
     1201 ggtgacggtc agggcaggac gctggctcgc cgtgccgtat tcactggagc gcatatcgac
     1261 gccatcgctg gtgccgccgc tgcggatggt gaagccgtta ttggccaggc tgccgttcac
     1321 ccatccctgc accagcgcaa tgcctgcggc attgagcgtg agcgtatgac tgccggtggc
     1381 ggtgggtgtg aacgatccga tctcggtgcc ctgactgccc gcgatatcgg cattggccca
     1441 ggttgcactg ccctcgctcc agctggcgtt gcgcgcatac agcctgtaag tgccggcgct
     1501 ggcgttggac acccgcaacg ttaccgtggc attggtgaca gacgcagacg ccggaatcga
     1561 cgccacgctc catttcaaca gcgccaccag ttcgccgttg ctgccatcgg caccgtccgc
     1621 cagcactgcg gctgtagtgc cgtaattgtt gccggttttg ccgctggcca cgtaggtgtc
     1681 cagggttcct gcgtagctgt tcaggccatc ccgcagcgtc actgtcaccg ggttggaccc
     1741 cgccgcctgg gtggtagcgc tggacacatt gctgtagccg gacgcaccgc caccattccg
     1801 ggcacgcacg cggtagaagt aagtggtgct ggctgccagt ccgccattgc tgtagctcac
     1861 cacgttgctg cccaccgagg ccagcgccgt ccagttggct ccgtccagcg aacgttcgat
     1921 atcgaaaccg gtttcgtcgc tggcgttgtc ggtccaggtc aggttgatgt tgctggatga
     1981 cgtggcggtt gcggccagac tggaaggcgc agcgggtggt gccggcggtg tgctgtcctt
     2041 ggtgatggtg aaataatccc gcaccgtgcc attgttgttc aggtatttca gatccatggt
     2101 ggcgccgttc acatcgatca ccatggaacc cagttcattc agcgaaaggt acatggccgg
     2161 atgattgagc gtgccgccgc tggtctggcc cgatgcgccc accacggaat acaccgcgcc
     2221 ggaatgcggc tggctggcat ccttcacgta ggcgccggag ccatctgcac ggccactgcc
     2281 accgtcgatt tcataggaac tgttgtaggt gctggacaag ccgtaatgac catcgatgaa
     2341 ctttgaccgc tcataggaat gactgtggcc ggtcagcacc agatccacgc cgtagttttc
     2401 cagcaccggc aaaaaattct gccgcatgtc gatcaggttg gattcggtgt cggaattgtg
     2461 cgagcccttg ctgtaaggcg gatggtgcca aaccgccacc agccattccg ccgtgttgtt
     2521 ggccagatcc gcctgcagcc agttcatcat ggcaccgctg gtggaacggc tggtttcgta
     2581 ggaatccagc accacgaaat ggacgttgcc gtaatcgaac gagtaatacg cctcagtgcc
     2641 ggacgccacg ccgcccactt caccgttgcg gggaaacgtg tgaatgttgt aataggggcc
     2701 ggtctggctg gcggaatcgg cgctggcgcc atcgtgattg cccagcgtgg cccataccgg
     2761 cgactggcgc agcagctcgg gatacatggc gaacagcttc gactggtatt cagcatcggt
     2821 gccgctgtta taggcattgt cgcccagcat cagccacaaa tccgtgtagc gggtgccggt
     2881 gaaattgcga taggcgttgt acaccgccgc ctggccggca ctgccggttc ccgcatcgcc
     2941 gatgacccag atccgggtgg gcacagccgc tcccggcgtg ggcgaggttt caaaataata
     3001 gctggcatcg ccggcgtgga ccgacgcgct gtcacctacg ttgtagaagt aacgggtgag
     3061 cggattcagg ccggtgacgg ccacttcatg ctcggtagtc accgtggagt tagtcgcaga
     3121 aaacgccagg ctgccctgac tggtaccgaa ctggacgcgg ctgttggtgg ccacgtcggt
     3181 gcgccagcgc actaccatac tgtcgctgtc gcccatctgc agataagggc cgcgcactac
     3241 cgtagccgcc aacgcggcgc cctgtgccag cgcgcaggtg agcacggcgc catagagggc
     3301 agcgtactta acccgggagt taaatgtcgt catttttatt ggctccagaa ttgataaaac
     3361 ggaatgggat gaaatctgcg agctgctgaa tcggcagggt tcagctgaag acgataggcg
     3421 ggggacttta acaatcgctg atagtttcgt gaatttttta tgcgtcagga tgacagtgcc
     3481 ctgacagcgc gcagggcact attccgcgca atactgcgtt acagctttgc ctgtttcgga
     3541 tgggaattgg acgcgctcgc gccaaagccg aacttcttgc gcagcgtgat atagaacttg
     3601 cccatcagat cactgttgga acccgccgca gactcgatga tcttcaggtg cccttcccgt
     3661 tcgctgaccg aaaactcctt gatgtacttg gcctggatct gttcgtaact gagccccttc
     3721 tgtttcatga accaggacag gaacagaccc acataggaaa tcatctcgcg ggtttttttc
     3781 atctcgaaat tgcgttgttc caccgcgcct tccgacagca ttttataaat gcccttctcg
     3841 atctgggcca gctggtcggt gctcaggctt ttatccttcg ccgcctcggc catcatctcg
     3901 tcgatcagct gtttggcgtt ggcaggcatg gttttcaggg gttgcgtgta cacccggtcg
     3961 cggatacgga tgtcgttgag cagatcgtag ctcaccttgg tgattaccat tgcggcggcg
     4021 gccatcatgt aggcgttcat gaaaagtccc tctggtgggt ggtcttcctg tggattgggg
     4081 gcaatgaaat ggggctggcc cggggattca gcgtaaacct gatagcggga actgcaatgt
     4141 gaaaaatacc gattattggc gtcgtgcctg tcgccataga ggaacgaggg tgttcaattt
     4201 ttcgcctgtt tgaaacgcca gcgtttcatg gtcattgtgg ttctcccttc gtttcagcca
     4261 catgcggcag ccgaccgcgt gctgcacgtt cgcgcgccag ctctgctgcc ctgtgcaact
     4321 gtgcttgcat cactttcatg tcaggcatgt gatcaaggta ttcagccaca cggatattgg
     4381 cactactcaa atccatcaac tctacctgct cggcgttggc acccgcacac aaaatcaggc
     4441 caatcggcga ctcttcaccc ggcgcacggt catacttgtc cagccagcgc aaata
//
LOCUS       DASUEG010000118         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1740193, whole genome shotgun sequence.
ACCESSION   DASUEG010000118 DASUEG010000000
VERSION     DASUEG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740193"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFX11_02135"
     CDS             <1..607
                     /locus_tag="VFX11_02135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4937442.1"
                     /translation="TMELLVQEARRRGLDQQARVAAEARLATGSWLARQMVQEYTRSI
                     QVDPVEILERYRELTPRREYRVSHILLNSRAQAQKIARQLRAGQDFTALADAESEDVM
                     AGPGGDLGWLMLNQMLASFADAVRTLQPGSVTAEPVASEFGWHIIRLENQRELPPPTF
                     EEVQLTLKDEIVQARLQRWMARLRIDADVELNSTAWLADAQ"
     gene            629..1207
                     /locus_tag="VFX11_02140"
     CDS             629..1207
                     /locus_tag="VFX11_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEX4937443.1"
                     /translation="MQPSMQRNIRVLIAVSVMLTSGLAVLVWAIRLEPRTAVAAQLIA
                     SAAATPAIPADIHIPAALLRESAGGDLMVDGATAQALDSVHAALSQRDDRAALSRLVN
                     QLRQTPGGAAGHQAATLLMHYHEYRKRMVEMSAGAAAADPAEVDAIQHDQARLRAAYF
                     SRRDAAVLFGDDAAASGRLTEPRLAGRNNVDQ"
     gene            complement(1201..1728)
                     /locus_tag="VFX11_02145"
     CDS             complement(1201..1728)
                     /locus_tag="VFX11_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEX4937444.1"
                     /translation="MTDSPRTPGPDAPGETRATPPGWFRRTRTGGSIFLTTLFDFSFS
                     KFITVQMFPVLYGVILASTLVGVIYLTVEAFFTSWWRGLFYLFVAGPIGFITVATITR
                     ALMEFYIVVFRIAENMDEIRVITERFSGISQGVESMRELTRLIPFWKTAGSKSGDSAD
                     KPDRTKRKKDQNWPY"
     gene            complement(1827..3176)
                     /locus_tag="VFX11_02150"
     CDS             complement(1827..3176)
                     /locus_tag="VFX11_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4937445.1"
                     /translation="MRRDSSRDLLTAPIGSTLTRMTIPTVIGIVAILFFNLADTYFIG
                     LMGPAELAAVSFTFPVCAMLMNVSMGLGIGTSIHISSRIGKGELAEARRTAAHALMLA
                     VTIAVPVGLLGILTIDPFFRLLGADSATIDLIRDYMGLWYAGFFLLVIPMVGNSVIRA
                     TGDTKTPSLIMGIAGLCNGVLDPLLIFGIGPFPELGIQGAVIATLVSWLMSGIAVLHV
                     LIRRLELMTPTDLLGPKLAHWRSILRVAIPATTTNLMTPLAGAVLTAMAAREGVHAVA
                     GYGVGTRLEAIALLVIMALSSALTPFVGQNFGAGQINRIRTAVKSALLFTVGWELLMA
                     LLLFLGGDHLAGLFSEDPETRVAIVWYLWLVPASYAFLGIVMVSCSALNALHRPMYGT
                     LISSLRLFALAVPLAWLGGWLYDTQGLYAGVALGNVLAGVGTLLWMRPYLRRLEAAA"
     gene            complement(3250..3663)
                     /locus_tag="VFX11_02155"
     CDS             complement(3250..3663)
                     /locus_tag="VFX11_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX4937446.1"
                     /translation="MKKLFNAIPVLVAMALASPVLAAGASKEEADPNGITYVELAPAF
                     VTNFVAPKIRYLKADVTLKVKGTATADAVNRHVPYIRNNLVMLFSRQEEEALNSPEGR
                     QHLKEEALQEVINALQAESEPVEVQEVLFTSFIVD"
     gene            3872..4552
                     /locus_tag="VFX11_02160"
     CDS             3872..4552
                     /locus_tag="VFX11_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX4937447.1"
                     /translation="MQFNALLFSPVLMLSAWLLAGVLLFAALRLAPWRPVLVVPLRQH
                     ALFAGLALLGLLWSFRFEVVPGFHCHPFLMVTLTLVFGWSFALLGGAVVALLVVVAGR
                     ADLQALPLDWLLTVVLPATLAYGLLQAVYRLRLRNLFFYILGLGFFGTIAITFVTCCA
                     VWLLLAATQTAPFMDELWEKSAVVVPLLYAEGFLNGVLVTAITVFVPDLVKTFDDRHF
                     LDGNGPDR"
     gene            4650..4928
                     /locus_tag="VFX11_02165"
     CDS             4650..4928
                     /locus_tag="VFX11_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937448.1"
                     /translation="MRTIHKFEIALNKEIQSIDMLDPPHYLRVEYLVHKRSIFLWAEV
                     PADLSVKRQTRRFRVFSTGDGIPDSARYVGTCVDQYLPESYHLYELLD"
     gene            5009..>5229
                     /locus_tag="VFX11_02170"
     CDS             5009..>5229
                     /locus_tag="VFX11_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX4937449.1"
                     /translation="MSQPQTLGKYQIQGILGKGAMGVVYKGFDPNIARLVAIKTIHAS
                     LLDTDMGRELLNRFRNEAQAVGRLNHANIV"
BASE COUNT          992 a         1579 c         1651 g         1007 t
ORIGIN      
        1 caccatggaa ttgctggtgc aggaagcccg ccgtcggggg ttggaccagc aggcgcgggt
       61 agcggccgaa gcgaggctgg ccaccggttc ctggctggcg cggcagatgg tgcaggaata
      121 tacccgttcg attcaggtcg atcccgttga aatccttgag cgttaccgcg agctgacgcc
      181 ccggcgcgag tatcgggtca gtcatattct gctgaactcc cgcgcacagg cgcagaagat
      241 cgcgcgccag ttgcgggccg ggcaggattt cactgcgctg gcagatgctg aatccgagga
      301 tgtgatggcg ggtccgggtg gtgatctggg ctggttgatg ctaaaccaga tgttggcgtc
      361 cttcgccgat gccgtacgta cattgcagcc gggcagcgtc accgccgaac ccgtggcttc
      421 ggagttcggc tggcatatca tccgtctgga aaatcagcgt gagctgccgc cgccgacgtt
      481 tgaagaggta cagttgacgc tgaaagacga aattgtgcag gcccgactgc agcgatggat
      541 ggcgcgcctg cgcattgatg ccgatgtgga actgaattcc accgcctggc tggcggatgc
      601 acaataacaa cgggaaaagg gacttctcat gcagccttca atgcaacgca atatccgggt
      661 attgatcgcc gtctctgtaa tgctgaccag cgggctcgcc gtgctggtgt gggccatccg
      721 gctggagcct cgcacagcgg ttgcggcgca gctgattgcg tctgcggccg ccactcccgc
      781 gattccggcc gacatccaca ttcctgcagc gctgttgcgg gagagtgccg gcggcgatct
      841 gatggtggat ggggctacgg cccaggcgct ggattccgtc catgcggccc tgtcgcagag
      901 ggatgacagg gctgcgctgt cgcgcctggt caaccagctg cggcagacgc cgggcggtgc
      961 agcgggacac caggcggcta ccctgctgat gcactatcac gaataccgga aacgcatggt
     1021 ggaaatgagc gctggcgcgg ctgccgccga tccggctgag gtggacgcaa tccagcacga
     1081 ccaggcacgc ctgcgcgcgg cctatttttc acggcgtgat gcagcagtcc tgttcggtga
     1141 tgatgccgcc gcgagcgggc ggctgacgga gcctcgcctg gcgggccgca acaatgtgga
     1201 tcagtaaggc caattctggt ccttcttgcg tttcgtccgg tcgggtttgt ccgcgctatc
     1261 gcctgacttg ctgcccgccg ttttccagaa cggaatcagc cgcgtcagtt cgcgcatgga
     1321 ttccacgccc tgcgagatgc cggagaaacg ttcggtgatc acccggattt catccatgtt
     1381 ttcggcaatg cggaagacca cgatgtaaaa ctccatcagc gcgcgggtga ttgtggccac
     1441 cgtgataaat ccgatgggcc ccgccacgaa cagatagaac agtccgcgcc accaggaagt
     1501 gaaaaacgcc tccaccgtca ggtagatcac acccaccagc gtggacgcca ggatcacacc
     1561 gtacagcacc gggaacatct gcacggtgat gaacttggaa aaggaaaagt cgaacagggt
     1621 ggtgaggaat atgctgccgc cggtgcgggt gcgtctgaac cagccggggg gcgttgcgcg
     1681 ggtttcgccc ggagcgtccg ggccgggggt gcgtggggaa tccgtcatgg gcgacttcct
     1741 gactgggcgg aatacaggtt acagagccgg aagcggttac tgtaccagaa gggccaacag
     1801 tagcagatcc cggtggcaat gtggcgtcag gccgctgctt caaggcgccg cagatacggc
     1861 cgcatccaga gcagtgtacc cacacccgcc agcacgttgc ccagcgcgac gccggcatac
     1921 agtccttgcg tgtcatacag ccagcccccc agccaggcca gcggcactgc cagggcaaac
     1981 aggcgcagac tgctgatgag cgtgccgtac atggggcggt gcagcgcatt cagggccgag
     2041 caactcacca tgacgatacc cagaaacgca tagcttgccg gcaccagcca cagataccag
     2101 acgatggcga cgcgggtttc cggatcttcg ctgaacaggc ccgccaggtg atcgccgccg
     2161 aggaacagca gcagtgccat caacagttcc caccccacgg tgaacagcag cgcgctcttc
     2221 accgccgtgc ggatgcgatt gatctggccc gcaccgaaat tctgcccgac gaagggtgtc
     2281 agcgccgacg acagcgccat gatcaccagc agcgcgatgg cctccagccg ggtgcccacg
     2341 ccatagcccg ccaccgcgtg cacgccttcg cgggcggcca tggcggtcag cacggcgccg
     2401 gccaggggcg tcatcaggtt ggtggtggtg gccggaatgg cgacccgcag tatcgagcgc
     2461 caatgcgcca gtttcggtcc cagcagatcg gtgggtgtca tcagttccag ccgcctgatc
     2521 agcacgtgca gcacggcaat gcctgacatc agccaggaca ccagcgtagc gatcaccgcg
     2581 ccttgaatgc caagctcggg aaacgggccg atgccgaaaa tcagcagcgg gtccagcacg
     2641 ccgttgcaca gcccggcgat gcccatgatc aggcttggcg tcttggtgtc tccggtggcg
     2701 cggatcacgc tgttgcccac catgggaatc accagcagga aaaagccggc gtaccacagc
     2761 cccatgtagt cgcggatcag gtcgatggtg gcgctgtcgg ctcccagcag gcggaaaaac
     2821 ggatcgatgg tgagaatgcc gagcaggccc acgggaacgg caatggtcac cgccagcatc
     2881 agcgcgtggg cagccgtgcg gcgggcctcc gccagttcac ccttgccgat gcgcgaggaa
     2941 atgtggatcg acgtgccgat gcccaatccc atcgacacgt tcatcagcat ggcgcagacg
     3001 ggaaaggtaa aactcacggc cgccagttcc gccgggccca tcaggccgat gaaataggtg
     3061 tctgccagat tgaaaaacag aatggcgaca atcccgatca cggtcggaat cgtcatgcgg
     3121 gtgagggtgg agccgatggg tgcggtgagc agatccctgc tggagtcccg gcgcattcaa
     3181 gtcacaggct gaattcgaag tgcgcgatta tcccacaggc gggtgctcct ccgcacccgc
     3241 cgtgaacggt cagtccacga tgaagctggt gaacagcacc tcctgcacct cgacgggttc
     3301 gctttcagcc tgcaatgcgt tgatcacttc ctgcagcgcc tcttctttca gatgctggcg
     3361 gccttcgggc gagttgagcg cttcttcttc ctggcgactg aacagcatca ccagattgtt
     3421 gcggatgtag ggcacgtgcc ggttgacggc atctgcagtg gcggtacctt taaccttcag
     3481 cgtgacgtca gccttcagat agcggatctt gggcgcgaca aaattggtga cgaaggcggg
     3541 agccagttcc acataggtga ttccgttagg gtcggcttct tccttgctcg cgccagcggc
     3601 cagcacaggg gaggcaaggg ccatggcaac cagcaccggg attgcgttga acagcttttt
     3661 cattgcggtg aatcctgctt gggtgtcgat gagtccgggt acccctcaag aatagttcgg
     3721 agggaaaaat ttgccgggat aatttgtgcg aatccggcac tggtgcacgt tgctgccgca
     3781 gtggatgaaa ttgcttgatg atcagatggt tgcagcgtat cgctgagcga atgcctgtat
     3841 attcagcgcc aagggtttat actcccgcgc catgcaattc aatgccctgt tgttttcccc
     3901 tgtcctgatg ctgtcggcct ggctgctggc cggtgtcctg ctgtttgcgg ccctccgtct
     3961 ggccccctgg cgaccagtgc tggtggtgcc cctgcgccag cacgccctgt ttgccgggct
     4021 ggccctgctg gggctgctct ggtccttccg tttcgaagtc gtgcccggct tccactgcca
     4081 tccgttcctg atggtcacgc tgaccctggt gttcggctgg agctttgcgc tgctgggtgg
     4141 ggccgtcgtc gccctgctgg tcgtggtggc tggacgggcg gatctgcagg ccctgccgct
     4201 ggactggctg ctcaccgtgg tactgcctgc cacactggcg tacggcctgc tgcaggcggt
     4261 gtaccggctg cgtctgcgca atctgttctt ttacatcctg gggctgggtt ttttcggcac
     4321 catcgccatc accttcgtca cctgttgtgc cgtctggctg ctgctggcgg ccacccagac
     4381 ggcgccgttc atggacgagt tgtgggaaaa aagtgccgtg gtggtgccgc tgctgtatgc
     4441 ggaaggattt ctcaacggcg tgctcgtcac cgccatcacg gtgttcgtgc cggatctggt
     4501 gaaaacgttt gatgatcgtc acttcctcga cggcaacggc ccggaccggt gagcctgata
     4561 ccgcggtatt tgccggcaaa atcgtttgac ctgatccagt cgtttcgcaa tcattgaacc
     4621 tgattccgct gaacggacga cagcccgcca tgagaaccat ccacaaattc gaaatcgccc
     4681 tcaacaagga aatccagtcc atcgacatgc tggatccgcc gcattacctg cgcgtggaat
     4741 acctggtgca caagcgcagc attttcctgt gggccgaggt gcccgccgac ctgtcggtga
     4801 aacgccagac ccggcgcttc cgcgtgttct ccaccggtga cggcatccct gacagcgccc
     4861 gttatgttgg cacctgcgtc gatcaatacc tgccggagtc ttatcatctc tacgaattgc
     4921 tggactgatc gcctataatc aacgggatct caaggggctg cggccccttt tttgcagaac
     4981 ggaataattc gcgcaacagg aaccccgcat gagccaaccg caaaccctcg gcaagtacca
     5041 gattcagggc attctgggta aaggcgccat gggagtggtg tacaaggggt tcgaccccaa
     5101 catcgcccgt ctggtggcga tcaagaccat tcatgcctcc ctgctggata ccgacatggg
     5161 gcgcgaactg ctcaaccggt tccgcaacga ggcgcaggca gtggggcggc tcaaccacgc
     5221 caacatcgt
//
LOCUS       DASUEG010000119         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_635383, whole genome shotgun sequence.
ACCESSION   DASUEG010000119 DASUEG010000000
VERSION     DASUEG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635383"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1142)
                     /locus_tag="VFX11_02175"
     CDS             complement(249..1142)
                     /locus_tag="VFX11_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937450.1"
                     /translation="MRVLQRILAFFVGWLNRQKEPREHALCDFDRVRSELRPGDVLLL
                     EGRSVAERILKTVTLSPWTHAALYIGRPMELEDSDLKNTLSNFFSAAPDTPLIIETRL
                     GCGVQVRPLLDLEHDHIRICRPKGLAPKDVQQVIRFAINRLGSFQNGIYLMDLTRFLF
                     PWGLLPSSWRLPLFRRWPGRHTKNLSSSFIAESFGFIQFPVYPLVKITTDQGTQLWRR
                     HPRLCLPYEIDMSPNFEIVKYPFLDFRQYDHDRMIPWKGSGLYSGVEQEPALTFQRPP
                     ATKPAADQPAGNVLPINLRSD"
     gene            complement(1149..2273)
                     /locus_tag="VFX11_02180"
     CDS             complement(1149..2273)
                     /locus_tag="VFX11_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4937451.1"
                     /translation="MDLNSEFPLDPELCYLNHAAVAPWPRRAAEAVARFAQDNGNRGA
                     ADYERWLETEQTLRQRLARLINAPSTRNIALQKNTSEGLSAIAWGLDWHPGDCVVISD
                     QEFPSNRIVWESLQRLGVQVVEATLATADPEQSIIDAMDGNTRLLSVSSVQYGTGLAL
                     DLVRLGEACRERGVLFCVDAIQSLGALPFDVSQCRADFVVADGHKWMLGPEGVALFYV
                     AEPHLERLQLHQFGWHMVRERGNYDIKTWTIASDAKRFECGSPNMLGIHGLEASLALL
                     EEIGGERILAELMTRVGVLEQLLREHPELELITVTDRPLRSGILTFRHRTMATPALFK
                     HLRDQQVVCAPRGGGIRFSPHFYTPLDVLERAVSLIPGKE"
     gene            2503..3144
                     /locus_tag="VFX11_02185"
     CDS             2503..3144
                     /locus_tag="VFX11_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937452.1"
                     /translation="MSDLQKASQKAQEFRRREEEILERAQELFIAKGEDKVTVEMIAD
                     AVNIGKGTIYKHFETKDEIYLRLMIRYEEQLADVFRRLDESDGKDRAQLAKDYFSFRM
                     KEPEKYALFDRLEHKLTNQTSCPDLVAKLHEIRAANLDQLAAAIKTRIDEGKLEDVPP
                     YFHIFAAWALVHGAVALYHSKFYQEFIKDKDGFFNFLAEIGVRMGNRRPQKGK"
     gene            3312..3872
                     /locus_tag="VFX11_02190"
     CDS             3312..3872
                     /locus_tag="VFX11_02190"
                     /inference="COORDINATES: protein motif:HMM:NF027431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4823 domain-containing protein"
                     /protein_id="HEX4937453.1"
                     /translation="MMGDWSPVGSQQITRQRQFVLPSHYRLYVGRLEGGLSTGDYPDL
                     NQTTSAELARYLRRYFVEVEMAGKSQSLASALKGAAAADADVLLLPRLESWPSIKPIG
                     SSDCGTKPEQAGAAPEACAESTDQQGELALSVAIYDVRARHQIDTVSAHGRRGAAAFL
                     TEKKFEDLQSLCSAIASQLAVQGRVE"
     gene            4024..>5210
                     /locus_tag="VFX11_02195"
     CDS             4024..>5210
                     /locus_tag="VFX11_02195"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4937454.1"
                     /translation="MKRFFQKLSIARKLVLINLVVVLAALCFAFILFVVLERAEAREQ
                     VVRELTAQTEIMANGVVSSLQAADPAQAQHVLGGFRVAANVREAILFDASRKVLAVYN
                     ASEDVPVEDTGFSYLHGGHLQWPGMGSRSVVFSREGLYLYQDIMQDDVVLGGLYVHST
                     LDHFHAYKSQAMLAIATVFAIALLMALLVMTRIMQVVTRPVQDLLQAVHTVRRDGNFS
                     VRVEKCADDELGELAQEFNTMLMAIGERDAELGKQHLRLEAEVQDRTRELKLTNESLE
                     ETVRALQQANRAIRISEENKRVAEASAQAKAHFLANMSHELRTPMNGVLGMLSLLNDT
                     TLNEEQRDYVNVAYESGHILLDLINNVLDLSKIDQGKLVLEAIVFDVRKSIEEVYSIL
                     AESA"
BASE COUNT         1111 a         1451 c         1573 g         1075 t
ORIGIN      
        1 cgatccacgg tacctggacc accgaagtga cccaaccaat gttatctctc acatttgggg
       61 ggaagtgagc aacatcagta agctaagtat tgcttaacat tctgaaacat ccagggtcct
      121 gttaaatcta tttgccatta aaaaacggcc gtaacatccg gaacggctat attcgttttt
      181 ttcgctgcaa aatcatgccg ctggctcgtg caagcgggga tttccgcagg tgcacgcccc
      241 gcgacctgct agtcggaacg caggttgatg ggcagcacgt tgccggccgg ttggtccgcc
      301 gcaggtttcg tcgcgggagg acgctggaag gtcagtgccg gttcctgctc cacgccggaa
      361 taaaggccgc tgcctttcca gggaatcatg cgatcgtggt catactggcg aaaatccagg
      421 aagggatatt tcacgatttc aaaattcggc gacatgtcga tttcgtacgg cagacacagg
      481 cgcggatgcc ggcgccacag ctgggtgccc tggtccgtgg tgattttcac cagcggataa
      541 accggaaact ggatgaagcc gaaggattcg gcgatgaagg acgatgacag attcttggtg
      601 tggcgcccgg gccagcgccg gaacagcggc aaccgccagc tggacggcag cagaccccag
      661 ggaaacagga agcgtgtaag gtccatcagg tagatgccgt tctggaatga accgagccgg
      721 ttgatcgcga agcggatcac ctgttgcaca tccttgggcg caaggccctt gggccggcag
      781 atgcggatgt ggtcatgttc aaggtccagc aacgggcgca cctgcacgcc gcagccgagc
      841 cgggtttcga tgatcagcgg ggtatccggc gcggcgctga aaaaattgct cagggtgttc
      901 ttcagatcgc tgtcttccag ctccatcgga cggccgatat agagggccgc atgggtccag
      961 ggactaagcg tgacggtttt gagaatccgc tccgcgacgc tgcgaccttc cagcaacagc
     1021 acatcgcctg gtcgcagctc gctgcgcacg cgatcgaaat cgcacagggc atgttcgcgc
     1081 ggttctttct gccggttcaa ccagccgacg aaaaaggcca gaatgcgctg cagtacacgc
     1141 atgatcgact actccttccc gggaatcagg ctcactgcac gctcaagcac gtccagcggt
     1201 gtatagaaat ggggtgagaa acggatgcca ccgccgcgcg gtgcgcacac cacctgctgg
     1261 tcgcgcagat gtttgaacag ggcaggtgtc gccatggtgc gatgccggaa cgtcaggatt
     1321 cccgaccgca gcgggcggtc tgtcactgtg atcagctcca gctctggatg ctcacgcagc
     1381 agctgctcca gtacgccaac gcgggtcatc agttccgcca ggattcgctc gccgccgatt
     1441 tcttccagca gcgccaggct tgcctccagc ccatggatgc ccagcatgtt ggggctgccg
     1501 cattcgaagc gtttggcatc gctggcaatg gtccaggtct tgatatcgta attgccccgc
     1561 tcgcgcacca tatgccagcc gaactgatgc agctgcaggc gttccagatg gggctcggcc
     1621 acgtagaaca acgcaacgcc ttcggggccc agcatccatt tatggccgtc tgccaccacg
     1681 aaatccgcgc ggcactggct gacatcgaag ggcagggcac ccaggctctg gatcgcatcc
     1741 acgcaaaaca gcacgccccg ctcccggcag gcctctccca gccgcaccag atcgagggcg
     1801 agaccggtgc cgtattgcac cgagctgact gacagcaaac gggtattgcc gtccatggcg
     1861 tcgatgatcg actgttccgg atccgccgtc gccagcgtcg cttccactac ctgcacaccc
     1921 aggcgttgca gggattccca gacaatgcgg ttgctgggaa actcctggtc gctgatcacc
     1981 acgcaatccc ccggatgcca gtccaggccc caggcaatgg ccgacaggcc ttccgatgtg
     2041 tttttctgca gggcgatgtt gcgggtggat ggcgcgttga tcagccgcgc cagtcgttgg
     2101 cgcagagttt gctccgtttc cagccagcgt tcgtaatcgg cagcaccgcg gttgccattg
     2161 tcctgggcaa aacgcgccac ggcctcggcg gcgcgtcggg gccagggcgc caccgccgca
     2221 tggttgaggt agcagagttc agggtcgagc ggaaattcag aattcagatc aatgcgcttc
     2281 aaggcaattg catccagctg gccagagtag aatagtctat agtgtttgaa ttggtgactc
     2341 caaggtcatc tttggtcgga caaagcaaag ttgtcgcaat tgtctgactt ataaggaatt
     2401 tgtggcaata aaaaaccgta ctttgctctc gcttggtaat aggaaatgag cgggaaattg
     2461 cgcataatcg cgcctgttgt tggatagttg gaggcttgtc ggatgagtga tttacaaaaa
     2521 gccagtcaaa aggctcagga attccgcaga agagaagagg aaattctgga gcgcgcccag
     2581 gaattgttta ttgccaaggg cgaagacaag gtgacggtgg aaatgattgc ggacgcagtc
     2641 aatatcggca agggcaccat ttacaagcac ttcgagacca aggacgaaat ctacctgcgg
     2701 ttgatgatcc ggtatgagga acagctggca gatgtattcc ggcgtctgga cgaaagcgat
     2761 ggcaaggacc gtgcccagct ggccaaggat tacttttcgt tccgcatgaa ggaacccgag
     2821 aaatacgcat tgttcgaccg tctggagcac aagctcacca accagacatc ctgcccggat
     2881 ctggtggcga agttgcacga aatccgcgcc gccaatctgg accagctggc agcggccatc
     2941 aagacccgca tcgacgaggg caagctggaa gatgtgccgc cctatttcca tatttttgct
     3001 gcctgggcat tggtgcacgg cgcggtggct ctgtatcact ccaagttcta tcaggaattc
     3061 atcaaggaca aggatggctt tttcaacttc ctggccgaaa tcggtgtccg catggggaac
     3121 cggcgtccgc agaagggcaa gtagtcggtt cctccgctgc gccgggcttc ttctatactg
     3181 gtttgcatgg caatgaacct gtaacgaaaa ggcccggaat cgtgcattcg attgttctgc
     3241 ttgtcattct tgttgtggtt gggcttgcgg gctgtgtttc gcgttcaacc actgccaatt
     3301 ccgccaccag tatgatgggg gactggtcgc cggtgggtag ccagcagatc acccgccagc
     3361 gccagtttgt gctgcccagt cattaccgcc tgtatgtggg ccggctggag ggcgggctgt
     3421 ccactggtga ttaccctgat ctgaaccaga ctaccagcgc tgaactggcc cgctatctgc
     3481 gccgttattt tgtggaagtg gaaatggcgg gcaagtccca gagcctggca tccgcgctca
     3541 agggcgcggc ggcggcggac gcggatgtgc tgttgctgcc ccgcctggaa agctggccca
     3601 gtatcaagcc gatcggatca tctgactgcg ggacaaaacc ggagcaggcc ggcgccgcgc
     3661 ccgaggcctg tgcagaatcc acggatcaac agggcgagct ggcgctgtca gtggcaattt
     3721 atgacgtgcg tgcccgtcat cagatcgata ctgtttctgc ccatggccgg cgcggcgcag
     3781 cggcgtttct caccgagaaa aaatttgagg atctgcagtc gctctgcagt gcgatcgcat
     3841 cgcagctggc cgtgcagggc agggtggaat aatacgtatg acgtcatcat tgtttgctgc
     3901 tatgatcccc gcgattgtta aacaatatga caaacactga cgcctttccg gatgttttct
     3961 taagctttcc aaactgctga tttccagatt tacggggtac accaggggaa acggggaagt
     4021 cccatgaaac gcttttttca gaagctttca attgcacgca agctggtact gatcaacctg
     4081 gtagtggtgc tggcggcact gtgctttgca ttcatcctgt ttgtggtgct ggagcgggca
     4141 gaagcccgcg agcaggtggt gcgcgaactc acggcgcaaa ccgagatcat ggccaacggc
     4201 gtcgtatcca gtctgcaggc tgccgatccc gcgcaagcgc agcatgtact gggcggattc
     4261 cgtgtcgccg ccaacgtgcg cgaagccatt ctgttcgatg ccagccgcaa ggtgctggct
     4321 gtgtacaacg cgtccgaaga cgtgccggtg gaagacacag gcttttccta tctgcacggt
     4381 ggccatctgc aatggccggg catgggatcg cgctcggtag tgttttcccg cgaagggctt
     4441 tatctgtatc aggacatcat gcaggacgat gtcgttctcg gcggccttta cgtccattcc
     4501 actctcgatc attttcatgc ctacaagagc caggcgatgc tggcaattgc cactgtgttt
     4561 gccattgccc tgctgatggc gctgttggtg atgacccgca tcatgcaggt ggtgacacgg
     4621 ccggtgcagg atctgctgca ggcggtacac acggtgcggc gcgacggcaa tttcagtgtg
     4681 cgggtcgaga agtgtgccga cgacgagctg ggtgagctgg cgcaggaatt caacaccatg
     4741 ctgatggcca tcggcgagcg tgacgcagag ctgggcaagc agcatctgcg actggaagcc
     4801 gaggtgcagg atcgcacccg cgaactcaaa ctcaccaacg aaagtctgga agaaaccgtg
     4861 cgcgcgttgc agcaagccaa ccgcgccatc cgcatttccg aggaaaacaa gcgggtggcc
     4921 gaggccagcg ctcaggccaa ggcgcatttc ctggccaaca tgagccatga gttgcgcaca
     4981 ccgatgaatg gtgtgctggg catgctgtcg ctgctcaatg acaccaccct gaacgaagag
     5041 cagcgcgatt atgtcaacgt ggcctatgaa tccgggcaca tattactgga cctgatcaac
     5101 aatgtgctcg acctgtcgaa gatcgaccag ggcaagctgg tgctggaagc catcgtgttc
     5161 gatgtgcgca aatccatcga ggaagtgtac tccattctgg cggaatcggc
//
LOCUS       DASUEG010000120         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_900383, whole genome shotgun sequence.
ACCESSION   DASUEG010000120 DASUEG010000000
VERSION     DASUEG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900383"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..847
                     /locus_tag="VFX11_02200"
     CDS             188..847
                     /locus_tag="VFX11_02200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4937455.1"
                     /translation="MPAALHLPRFTPAQHVVLYVDDVLMVVNKPARLLSVPGRGEDKQ
                     DCLIARLQLDYPDARIVHRLDYDTSGLLVMGRNADSHRELSRQFQDREVDKQYIALAL
                     GYVAEDSGEIDLPIRYDPPTKPRQVVDMINGQPALTRWKVLDRRQGHTRLALEPFTGR
                     THQLRVHMQALGHPLLGDALYAPEEARSTESRLCLHAETLAFTHPVHRGRLKFRTPAP
                     F"
     gene            869..>3432
                     /gene="rapA"
                     /locus_tag="VFX11_02205"
     CDS             869..>3432
                     /gene="rapA"
                     /locus_tag="VFX11_02205"
                     /inference="COORDINATES: protein motif:HMM:NF003426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEX4937456.1"
                     /translation="MSSSTSVFTPAMGQRWISDSETELGLGVIIETDARSLTVLFPKS
                     DETRVYARNNAPLSRIRFAEGDTVTDVGGQQWKVVSVEEQRGLLRYHVEGAAGDSTVL
                     NETRLSADIHLARPQERLLACQLDSIDLFDLRVQALLQQEKLAQSPVRGLVGPRLGRV
                     PHQFYIAHDVGLRPQPRVLLGDEVGLGKTIEAGLIIHHQLQTGRAGRVLILVPENLQY
                     QWLVEMRRRFNLNAALFDLERCAALRESDEEANPFETEQIVLMALELLVDHPDLADAV
                     RNTAWDMLVVDEAHHLEWTPEAPSDAYALVASLAETIPGVLLLTATPEHLGVASHFAR
                     LRLLDPARYHDLEQFIAEEEAFASVASAADALLGEEPLDPTALAVLQEKLPADLIAHL
                     DTDEGRQSALTALLDRHGVGRVLFRNTREAIGGFPERRCLPVPLPAPADAATTELRVA
                     LYPEMHGDDDWCEGDARVEWLISLLKSLKRDKVLLICRSSPVALALEEYLRLKEGVRT
                     SVFHEGMSLLERDRAAAYFADEDYGAQILICSEIGSEGRNFQFAHHLVLFDLPADPEL
                     LEQRIGRLDRIGQRETIKLHVPYFAGTAQERLFRWYHEGLNAFESISPTARTILEAQR
                     PQIPDALRNGGDTFERLLDDARTLRATLLAELEKGRDRLIELNSCRPDVAASLIESID
                     NQDDDPALSSFLERAWSAFGVDQEEQQETHLHILRPGDHQLLDGFPGLDPEGMTVSLD
                     RDYALAREDVHFLTWEHPMAQGLLDLFRSQEYGNANVALIRNKGIKPGTMLLELFFRL
                     ETVAPKGLGVDAALPSQTLRVLLDQEGRDLSARVAHEVLAKQLQSLERGVARQVVKS"
BASE COUNT          655 a         1156 c          986 g          635 t
ORIGIN      
        1 gccatggtga caacggcgaa gaagagcaac ccatgaactc ggcgtgatga tgagagggat
       61 tcaggcatga tgccggattc tgcccgccac cttgcttgaa aagaagcgtt ccggcagaaa
      121 accggtgccg aaaatggccg gctcctctac aatccgcgct cccggacacg accagccgcc
      181 tgactgcatg cccgccgcgc tccacctccc ccgcttcacc cccgcccagc acgtggtgct
      241 ttacgtggat gacgtcctta tggtcgtcaa caagccggct cgcctgctca gcgttccagg
      301 ccgtggcgag gacaagcagg actgcctgat tgcccgcctc cagctcgact atcccgatgc
      361 ccgtatcgtg caccgtctcg actacgacac ctcgggtctg ctggttatgg gccgcaacgc
      421 cgactcacac cgtgagctgt cccggcagtt tcaggaccgg gaagtcgaca agcagtacat
      481 tgcccttgcg ctgggctatg tcgccgaaga ttcgggggag atcgacctcc ccattcgcta
      541 cgacccgccg acaaaaccac gccaggttgt ggacatgatc aatggccagc ctgcgctgac
      601 gcgctggaaa gtgcttgatc gccgtcaggg ccatacacgg cttgcgctgg aaccgtttac
      661 cggacgcacg catcaactgc gcgtgcacat gcaggcactg ggtcatcccc tgctgggtga
      721 tgccctttat gcgcctgaag aagcccgctc aaccgaaagc cggctctgcc tgcatgcaga
      781 aacccttgcc ttcacccatc ctgttcatcg tggccgcctg aagttccgca ccccggcccc
      841 attctgacta cccgagattg agacaagcat gagttcgtcg acctctgtgt tcacgcctgc
      901 catgggccaa cgctggattt ccgattccga aacggaactg ggcctgggcg tcatcattga
      961 aaccgatgcc cgttccctga ccgtgttgtt tcccaagagc gacgaaaccc gggtgtatgc
     1021 ccgcaacaat gcccccctct cccgcatccg cttcgcggag ggtgacaccg ttaccgacgt
     1081 tggcggacag caatggaaag tggtcagtgt cgaggaacaa cgcggcctgc tgcgttatca
     1141 cgtcgaaggc gctgccggtg acagcacggt actgaacgaa acccgtctct ccgccgacat
     1201 tcatctggcc cgcccccaag agcgcctgct ggcctgccag ctcgacagca tcgacctgtt
     1261 cgacctgcgt gtgcaggccc tgctgcaaca ggaaaagctg gcccagtctc cggtacgcgg
     1321 gctggtcgga ccgcgcctcg gccgtgtgcc gcaccagttc tacattgccc atgatgtcgg
     1381 tcttcgtccg caaccccgcg tcctgctcgg tgacgaagtc ggccttggca agaccatcga
     1441 agccggcctc atcattcatc accagttgca gaccggccgt gccgggcgcg tgctcatcct
     1501 ggtgcccgaa aacctgcagt accagtggct ggtggaaatg cgccgccgct tcaacctgaa
     1561 tgctgcgctg ttcgaccttg agcgttgcgc tgcccttcgc gagagcgatg aagaagccaa
     1621 tcccttcgaa accgaacaga ttgtgctgat ggcgctggag ttgctggtcg accaccccga
     1681 tctcgccgac gccgtacgca ataccgcgtg ggacatgctg gtcgtcgacg aagcccatca
     1741 tctggaatgg acacccgagg cgcccagcga cgcctacgcg ctggttgcct cgcttgccga
     1801 aacgattcct ggcgtactgc tgctgaccgc aacaccagaa catctgggcg tcgccagcca
     1861 ctttgcccga ctgcgcctgc tcgacccggc ccgttaccac gaccttgaac agttcatcgc
     1921 cgaagaagaa gcctttgcca gtgtcgccag cgcagccgac gccttgctgg gtgaagagcc
     1981 cctggacccg acggcccttg ccgttctgca ggaaaaactg ccagccgatc tgatcgccca
     2041 tctggacact gacgaaggac ggcagtcagc cctgaccgca ttgctggatc gccatggtgt
     2101 cgggcgcgta ctgttccgca acacccgcga agccatcggc ggcttcccgg aacgccgctg
     2161 cctgcccgtg cccttgcctg ccccggctga tgccgccacc accgagctgc gcgtcgcgct
     2221 gtacccggaa atgcacggcg atgacgactg gtgcgaaggc gacgcccgcg tcgaatggtt
     2281 gatcagcctg ctgaaatcgc tcaagcgcga caaggtgctg ctgatctgcc gctccagccc
     2341 tgttgcgctg gcgctggaag agtatctccg gctgaaggaa ggggtacgta cctctgtctt
     2401 ccacgaaggc atgagcctgc tggaacgaga ccgcgccgcc gcctattttg cagatgaaga
     2461 ttacggtgcc cagatcctga tctgctccga aatcggctcg gaaggccgca acttccagtt
     2521 cgcccatcat ctggtgctgt tcgacctgcc cgccgacccc gaattgctgg agcagcgcat
     2581 cggccgactg gaccgcatcg gccagcgcga aaccatcaag ctgcatgtac cttattttgc
     2641 cggcaccgcc caggaacgcc tcttccggtg gtaccacgag ggtctgaatg cctttgaaag
     2701 catcagcccg actgcccgca ccatcctgga agcgcagcgc ccgcaaattc cggatgccct
     2761 gcgcaatggt ggcgacacct tcgaacgcct gctggacgat gcccggaccc tgcgagccac
     2821 cctgttggcc gaactggaga agggtcgcga ccgtctgatc gaactgaact cctgccgtcc
     2881 cgatgttgcg gccagtctga tcgagtccat cgacaaccag gatgacgatc cggcactttc
     2941 cagcttcctt gaacgcgcct ggtccgcctt tggagtcgat caggaagagc agcaggaaac
     3001 ccatctgcac atcctgcgtc ccggcgacca ccagctgctg gatggattcc ccggcctgga
     3061 cccggaaggc atgaccgtct cactggatcg cgattacgcg ctggcccgtg aagacgtgca
     3121 cttcctcacc tgggaacatc cgatggccca gggcctgctg gacctgttcc gtagccagga
     3181 atacggcaac gccaatgtgg ccctgatccg caacaagggc atcaaacccg gcaccatgct
     3241 gctggagctt ttcttccgac tggaaaccgt ggcacccaag gggctggggg tagacgccgc
     3301 acttccctcg cagacattgc gtgtgctgct ggaccaggag gggcgggacc tctctgcgcg
     3361 tgtggcgcac gaggtattgg ccaagcaact gcaatcgctg gagcgcggcg tggcacgtca
     3421 ggttgtgaaa tc
//
LOCUS       DASUEG010000121         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_458715, whole genome shotgun sequence.
ACCESSION   DASUEG010000121 DASUEG010000000
VERSION     DASUEG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458715"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFX11_02210"
     CDS             complement(<1..870)
                     /locus_tag="VFX11_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937457.1"
                     /translation="MLKTDARIFVLLVLLLLARGAGAATCADVWPGAMTANSTVAPTL
                     PAFTGSSALTLSRTLAAGDFHYSSSSIASGTLTTSSATTRLYFNGSLSLSGTTLLNGG
                     GNPQNLIVIVNGSLSLANSARINGLVYATGSITLANTASITGAATAAGGIATSGSSSV
                     TYSENGVVGASYGTLCTPPVQPALKIVSPICSVSNKLIVTFNSSGGRLPLGSSATTLG
                     NYSLVSETGTVHTITGARLSDEGYEVQLTFTPTLVNGRDYTLSVANVKDQDGITMVSA
                     SDGFYFSSTTNGIV"
     gene            complement(857..1252)
                     /locus_tag="VFX11_02215"
     CDS             complement(857..1252)
                     /locus_tag="VFX11_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937458.1"
                     /translation="MKQYSPDKGFALPAVIFMMVIVVLLITAMTRLLSTQSAISDMQL
                     LGVRAFWAAKAGAEWAAYRINATHACATGNLAINGFQVTVGCVSNSYSEAGNTVSVFE
                     VNVSAQFGSDPGGVDYVSRQLNLVLNVEN"
     gene            complement(1249..2124)
                     /locus_tag="VFX11_02220"
     CDS             complement(1249..2124)
                     /locus_tag="VFX11_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4937459.1"
                     /translation="MNRRPPAAEIALHPAAGFTLVELVIVITLTGVVAVLASVLVGNQ
                     MLGYVDTARRAGLMAKADLALQHIARDLRGAVPYSIRVSGGAALEWVPIQSWGRYRKL
                     PDTSEDAALDFSEPDAQFEVLFGSAMPAIASGMQLVIGNTDAAGIDGINLYGDVSSGA
                     LVPAGSNVITPTSVIVGSSGSNITLTPAFQFALASLASRFYLVNGAASYVCSAGAVTR
                     YTGYPLQKSQPVNGAAAPLGTAASALLLDGVSSCQFGYTALDSTYGVVSVQLQLQDGS
                     ETVNMTRMITIENRP"
BASE COUNT          443 a          679 c          648 g          397 t
ORIGIN      
        1 gacgatgccg ttggtggtgc tggaaaaata gaagccgtcg ctggcggaca ccatggtgat
       61 gccgtcctgg tccttaacat tcgccaccga cagggtgtag tcgcggccgt tcaccagcgt
      121 cggggtgaag gtcagctgca cttcgtaacc ctcgtccgac aaccgcgccc cggtgatcgt
      181 gtgcacggtg ccggtttcac ttaccagcga gtagttgccg agcgtcgtgg cgctgctgcc
      241 cagcggcaaa cggccaccac tgctgttgaa ggtgacgatc agcttgttgc tgacactgca
      301 aatggggctg acgattttca gcgcgggctg caccggtggc gtgcagagag tgccgtagct
      361 ggcaccaacg actccattct cgctgtaggt gacgctggac gatccactcg tggcaatgcc
      421 gccggcagcg gtcgcagcgc cggtaatgct ggccgtgttg gcgagggtaa tgctccccgt
      481 tgcgtatacc aggccgttga tgcgcgcgct gttggccagc gacaacgaac cgttcacgat
      541 cacaatcaga ttctgcggat ttccaccccc gttcagcaag gtggtaccgg acagggacag
      601 actgccgttg aagtacagcc gggtggtggc ggatgacgta gtaagcgtcc cgctggcaat
      661 actggagctg ctgtaatgaa aatcccccgc cgccagcgtg cgcgagagcg tcagcgcgga
      721 actgccggta aatgcgggca atgtcggcgc caccgtactg ttggcggtca tggcacccgg
      781 ccagacgtcg gcgcaggtgg ccgctccagc gccacgggcc agcagcaaca acaccagcag
      841 cacaaaaatc cgcgcgtcag ttttcaacat tcagcaccag attcagttgt cgcgaaacgt
      901 agtccacacc gcccggatca cttccaaatt gcgccgacac atttacttca aacaccgaca
      961 ccgtattacc cgcttcgctg tagctgttcg atacgcagcc caccgtgacc tgaaaaccgt
     1021 tgatggcgag attgccggtg gcgcaggcgt gggtggcatt gatgcggtat gcggcccact
     1081 ccgcaccggc cttggcagcc cagaaggcgc gtacgcccag caactgcatg tcgctgatgg
     1141 cggactgggt gctgagcagg cgcgtcatcg ccgtgatcag caacaccacg atcaccatca
     1201 tgaaaatcac ggcaggcagg gcgaatccct tatccggcga gtactgcttc atggtcggtt
     1261 ctcgatggtg atcatgcggg tcatgttcac ggtctcggag ccatcctgca attgcagctg
     1321 caccgatacc acaccgtaag tcgaatccag tgcggtatac ccgaactggc aactgctgac
     1381 gccatccagc agcagggccg aggccgccgt tcccagtggc gcggctgcgc cattgaccgg
     1441 ttgcgatttt tgcagcggat agcccgtgta gcgcgtcacc gcgccggcac tgcaaacata
     1501 actggcggcg ccgttcacca gataaaaccg gctggccaat gatgccagcg caaactggaa
     1561 cgccggtgtg agcgtaatat tgctaccgct gctgccaacg atcaccgagg tgggtgtaat
     1621 cacattgcta cccgcgggca ccagcgcgcc actgctgaca tcgccataca ggttgatgcc
     1681 gtcaatgccg gccgcatcgg tattgccgat caccagctgc atgccgctgg caatggcggg
     1741 catcgcgctg ccgaacagca cttcaaactg tgcatccggc tcggagaaat ccagcgctgc
     1801 atcctcactg gtatccggca gcttgcggta gcggccccag ctctggatcg gcacccactc
     1861 cagcgcggca ccaccggaaa cacgaatgct gtacggcact gcgccacgca gatcgcgggc
     1921 gatatgctgc agtgcaagat ccgctttcgc catcaggccg gcacggcggg cggtatcgac
     1981 ataccccagc atctgattgc ccaccagaac cgatgccagc accgcgacca ctcccgtcag
     2041 cgtgatgaca atcaccagtt ccaccagcgt gaaaccggcc gcaggatgca atgcaatttc
     2101 agctgcaggg gggcgtcgat tcataatcgg tccggtagcc tgtcagaacg gtggcttcat
     2161 gcagcgg
//
LOCUS       DASUEG010000122         9999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_524936, whole genome shotgun sequence.
ACCESSION   DASUEG010000122 DASUEG010000000
VERSION     DASUEG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524936"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2776
                     /locus_tag="VFX11_02225"
     CDS             <1..2776
                     /locus_tag="VFX11_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX4937460.1"
                     /translation="PQRAYSYTAGLIDGIDQFDPAFFGISPREARGMDPQQRLVLQSC
                     VQALAHAGYAWEALRNSSTGVFVGVAANEYNRFQDLFNAGEDASHAASGNALNVIAGR
                     VAYTLGLQGPAIALDTACSSSLVALHTAVESLHAGDCEQAIVGGVNVILSPATFMLLC
                     QGQMLAPDGRCKTFDKDANGYVRAEAVATVILKPLAQARKDGDRILACIKGSAVNQDG
                     RSAGLTVPSSTAQQAVIEAALARAKLSPEQIEYVECHGTGTALGDPIEVQALAQAYCS
                     SQRTPLTLGAAKSCIGHAESAAGLVGLIKAVLALQADCIPPQAHFNQWNPLIHVDPAQ
                     LRIPTSALPWPRRNDVLRRCAVSAFGFSGTNAHVILEEYRAEADSPQRAPLAPVQFNQ
                     QRYWWRHPVDPITASTHAAATQVTAASASQQQHATTLEDAVVLNVARISGMEATALQP
                     AMSLSADLGFDSLMTGSLRDQLSRMLRHPQHDRVPLQILFGQDSLAEIVAALQSAGFA
                     LAQSGVVEENLAQDIHTGTDAAGQWDEKTQAYFSTRTALFDADRPSRIDRKLVHKSLD
                     ENVLVADLVQLSEKEFIAEMTQDRRHGYFYEHFLDHVPGLYVVEAVRQAATALCHLYR
                     GVDYSHAFILNRLSVSFSHFIETDQPAFIHLRITDETVRDGRVKLMEVDARVVHQGRE
                     VARISGGGDVSSRDEYAGYRGEALAAATVAAKSAQVHVTGSNEKQTGRVIACFDLDGT
                     LTHSHTFWRFLLRCAGSGRFVGSILRSLPILPAVWRGRMDLMQAREVLIERCLRGVSE
                     QKVQAVAARLTDQIATRLLRDGALDAVRRHQQLGHETFIVSNSAEYYLSLLAQRLQMN
                     GVFGSRFEVVDGHLTGKLQGTHCQGAEKVARVQSVLPDRATAQVYAYGDSSGDIAMLD
                     WADFPFLKRF"
     gene            2818..4545
                     /locus_tag="VFX11_02230"
     CDS             2818..4545
                     /locus_tag="VFX11_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4937461.1"
                     /translation="MDSMTHTHVCIVGAGPSGLTLANELQRYGVPFELIERRTERTVD
                     SKAFALHARSLEVFRRMGVADAILARGLPVAGMAAWAGKRRVLEGDLRLLNSEFPCVL
                     SIPQADTEAVLLDTLLESGGTVHWGQTLTAVRQNALQAQVDVRDNDGNTRTIACRWLV
                     GCDGAHSQVRRLANIDWSGDDYDFEFMVADGNIEWSGDLESGHTFLNRQGYVMLFPLP
                     KGRHRIVINVDVGRFSREELSAELVNRILQDRGMGDVTFSNPFWLSSTQGRRRIAGSF
                     RSGRVLLAGDAAHVHSPLGGQGLNTGIQDAYQLAWRLAAIRNCVDAEHPLLSAYALER
                     RQVAQEVLKNTDKLTRLFTARAPLALLVRNYVIPLLSRRRAFNISLAQTASGVGIRLA
                     QPALALRGPVNAKLAGTRLLHASINELYSATPVLMGNVIARDAFELVLLLGKAQTPAF
                     YEAKRCLAQLRNAVPHPADQHRNAVAIEPRRSLIVTPAIEPMLATWPEDLTDTRYLDV
                     SGAVLQHYGINDAGYLLVRPDGYIACGGPLLNAEDLIHNLGAVLHWPSTSATSFPQPI
                     ATPHQLAEV"
     gene            4547..6865
                     /locus_tag="VFX11_02235"
     CDS             4547..6865
                     /locus_tag="VFX11_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4937462.1"
                     /translation="MKFIGDFIVFRPITTLLIMFLLSAAAISGVTKLETRNNQDSELP
                     DTDPIVATKHNIDAIFGDKGVVVIGIEADNVYNPSTLQKIKDISEELKSVQYVLPDQI
                     ASLASANNVKSRDWGLDVGPFMRDVPTTPEEIEQLKADVRANTLVDGRLVTRDGTFTL
                     ISANVEEDYDQATVFDQVYKIVEKYQGPEKIYANGDPIFTQEIELGLQRDADVLVPIA
                     LLIIMIGLYFFFRTLRGVFLPALVIVLCIGWIMGLMGHLGLPQTVVSTAIPLLMVAIA
                     TSYGVHALLMYYEETVGQDQKTAVRNMLAKLFPVLSFVAFTTLLGTSSLVVFDVLSIR
                     EFAVEVMIGTGVAYLMTVTVIPAALSLMKAPTKVPTVVQENWLDGALVRLTEFSVRHK
                     YKVLGGYGVLVAICVVGIVQLKTGVDVTAVFPSDHRGRVSFDKFSEKLGGGRYFNVMV
                     EAPDAEGIKDPALLKTILDYQQFIEQQAGVGYTYSFANVVRHISHLVNENYSGNDIPA
                     TREELAQCLLLYDMSAQPGEFTDLVDNQYQRAKIRVMLDTSDPDDHIRLYEMSRAYLE
                     QHLPDNVKIEFGGDTMFWIAQVRYVVIGKIENIISGILLIVLFVTVISRSFVAGMISI
                     SPAVIGSVLAFGVMGFSGIRLEISAALITSIAIGIGTDFSIHYLGRLREFMPTSASID
                     EALVKVSRSNGKAIVYDTASNVLGFAVLLLSGFLQIRTFGSLVSLTMIVMAAGTLMLI
                     PALVAAIQPAYLYKRREVSEPPVTPAPALSAE"
     gene            6939..7754
                     /locus_tag="VFX11_02240"
     CDS             6939..7754
                     /locus_tag="VFX11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX4937463.1"
                     /translation="MFKYNRIAAALLAGFVATAAQASLEMDPREIMRKNEDARKSLNE
                     SAQMKMTLLDKSNNARYRWIDFIADDTDPFNRKSLIRFSQPNDIKGTGFLSIEQGNEE
                     DDLRWLYLPSLRKIRRISAGNKTDSFMGTDWWYEDFEFVDGVAKGGNNNYKILREEEM
                     NGARCWVIEAIPTTEQEIKESGYSKRLMWVRQDNYVLTREEKFDKKGELAKIQIATEI
                     KQIPGEENAWRAHRLEMVTVKTGHKTLIEYSSYQANVALPEGAFSTRYLEQAR"
     gene            7806..9155
                     /locus_tag="VFX11_02245"
     CDS             7806..9155
                     /locus_tag="VFX11_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX4937464.1"
                     /translation="MSTTRLAVTGLLLMPYAAMAADYEFRTRFEFEAAQNVAEGYAQK
                     MEFQARPELHVFPDADSEITAIARLRADAEDRLEVGRPLQEEVSEYSRRAIIGSHGDA
                     ELRELYYKHRFGQSYLTVGKQQVVWGTADGLRVLDVVNPFSFREFLLEDFDEARIPLW
                     MVNYIHSIGSDTDLQLLVIPDLTFNDFPDGDGDDAPEYEITSPRFQPGFPADVPPNLA
                     GVTNLGLQRPSDSLQNADAGLRLSTRLGELDLTFNYLYQYDDNPVPYRRINTASGQPV
                     LEFWQEYERTHLLGSTLSTDLSSTLVLRYEIGLVHGRRVLRENLNNDNGVLNTDELSY
                     VLGLDWSGVSDTLFSVQLFQSVLKDDAPDLVRDRVDSTMTFNVNSRFFNDTLTLEFMA
                     LHNLNDHDDLIRPSVTYAVRDGVNLWLGADIFTGEEAGVFGQFGETDRVLAGVEWYW"
     gene            complement(9158..>9999)
                     /locus_tag="VFX11_02250"
     CDS             complement(9158..>9999)
                     /locus_tag="VFX11_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937465.1"
                     /translation="LVLRGEGFDLLWRHNSQPSIQEWVVPSGHWVLHLPHPASDALTA
                     FQAHPEGYRHSLLGLPGDEPVWHMTAGQPCLVLVTRTQILESIFRAEELESLQQQTRT
                     LRRRQLDGQRLLAVSAALLAHLQALCPSLTPVAGPVDTGLTSLIRLAYSPLTITQPGE
                     PRISNRDRILTQALDFIRRHYARDICIADIVNHVHTTTRNLQIVFKAQFGMSPLQFLK
                     RYRLSRFHRELRRHGTVTAAAVYSGLRHMGRVSDEYRKLFGRNPLESLNRFRLENADL
                     VLQ"
BASE COUNT         2001 a         2904 c         3079 g         2015 t
ORIGIN      
        1 gccgcaacgc gcctacagct acacggccgg gctgatcgat ggcatcgacc agttcgatcc
       61 cgcctttttc ggcatttcgc cccgggaagc gcgcggcatg gacccgcagc agcgtctggt
      121 gctgcaatcc tgcgtgcagg cgctggcgca cgccggttat gcctgggaag cgttgcgcaa
      181 ttccagcacc ggcgtgttcg tgggcgtggc cgccaatgaa tacaatcggt ttcaggattt
      241 gtttaatgca ggtgaggatg cgagccatgc tgcatccggc aatgcactca acgtgatcgc
      301 cgggcgcgtc gcctacaccc tgggcctgca aggaccggcc attgcgctcg acaccgcctg
      361 ctcgtcttcg ctggtggcgt tgcacacggc ggtggaaagt ctgcacgcgg gtgattgcga
      421 acaggcgatt gtcggtggcg tcaatgtgat cctgtcgccg gctaccttca tgctgctgtg
      481 ccagggccag atgctggcac ccgatggccg ctgcaagacc ttcgacaagg atgcgaacgg
      541 ttatgtgcgg gccgaggcgg ttgccaccgt gattctgaaa ccactggcgc aggcgcgaaa
      601 agatggcgat cgcatcctgg cctgtatcaa gggcagcgca gtgaatcagg atggccgcag
      661 tgccggcctc accgtgccca gttccaccgc ccaacaggcg gtgattgaag cggcgctggc
      721 gcgggcgaag ctgtcgccag aacagatcga gtatgtggaa tgtcatggca ctggaacggc
      781 tctgggtgat ccaattgaag tgcaggcgtt ggctcaggcc tactgcagca gccagcgtac
      841 gcccctgaca cttggcgcgg caaagagctg catcggccat gcagaatctg ccgccggtct
      901 ggtggggctg atcaaagccg tgctggcatt gcaggcagac tgcattccgc cgcaggcgca
      961 tttcaatcag tggaacccgc tgatccatgt ggaccctgca cagctgcgca ttcccacgtc
     1021 tgcgctgccc tggccgcgcc ggaatgatgt gttgcgtcgc tgcgcggtga gtgcctttgg
     1081 tttcagcggc accaacgccc atgtaattct ggaagaatac cgcgcggaag cggatagccc
     1141 ccagcgcgca ccactggccc cggtacagtt caaccagcag cggtactggt ggcgtcaccc
     1201 cgtggacccg atcaccgcca gcacgcacgc agccgcaacg caggtaacag ccgccagtgc
     1261 atcgcaacag cagcatgcca cgacgctgga agacgcggtg gtgctcaatg tcgcccgcat
     1321 cagcggtatg gaagcaacag ccttgcaacc ggccatgtcg ctgagcgctg atctgggttt
     1381 cgattccctg atgaccggtt ccctgcggga tcagctcagc cgtatgctgc gccatccaca
     1441 gcatgatcgc gttccgctgc aaatactgtt cggccaggac agtctggcgg aaattgttgc
     1501 tgcactgcag tcggcaggat ttgcgttggc ccagtctggc gtagtcgagg agaacctggc
     1561 acaggacata cacaccggca cggatgccgc tggtcaatgg gatgaaaaga cacaggcgta
     1621 tttttccacc cgcacggcct tgttcgacgc cgatcgcccc agccgtattg accgtaaact
     1681 ggtgcacaag tcgctcgacg agaacgtgct ggttgccgat ctggtgcagt tgagcgaaaa
     1741 ggaatttatc gcggaaatga cacaggaccg tcgtcacggc tatttctacg agcatttcct
     1801 ggaccatgtt cccggcctgt acgtggtgga agccgtgcga caggccgcca cagcgctgtg
     1861 ccatctgtat cgtggcgtcg attattccca cgcttttatc ctgaaccggt tgtcggtctc
     1921 gttttcccat ttcatcgaaa cagatcagcc ggcgttcatt cacctgcgca tcaccgatga
     1981 aaccgtgcgc gatggtcgcg tgaaactgat ggaggtggat gcccgggtag tgcatcaggg
     2041 gcgggaagtg gcgcgcatca gcggcggtgg cgatgtgtcg tcgcgggatg agtatgcggg
     2101 ttaccgtggt gaggcgctgg cggcagccac cgtggcggcg aaatcggcgc aagtgcacgt
     2161 tactggcagc aacgaaaaac aaaccgggcg cgtgatcgcc tgtttcgatc tggatggcac
     2221 gctgactcac agtcacacct tctggcgttt cctgctgcgc tgcgctggca gcggacgctt
     2281 cgtgggttcc attctgcgtt cattgccgat cctgccagcg gtgtggcgtg gccgcatgga
     2341 tttgatgcag gcacgggagg tgctgatcga gcgctgcctg cgcggtgtgt ccgagcagaa
     2401 agtgcaggcg gtggcggcac gtcttaccga ccagatcgcc acccgtctgc tgcgtgatgg
     2461 cgcactggat gcggtgcgca ggcaccagca gctgggacat gaaactttca tcgtatccaa
     2521 ctccgccgaa tattacctga gcctgctggc gcagcggctg cagatgaacg gtgtattcgg
     2581 cagccgtttc gaggtggttg atggccatct caccgggaaa ctgcagggga cccattgcca
     2641 gggcgcggaa aaagtggcac gcgtgcaatc ggtgctgccg gatcgtgcca cggctcaggt
     2701 gtacgcctac ggtgacagca gcggcgacat tgccatgctg gactgggccg actttccgtt
     2761 tctgaaacgc ttctagacgc acttatcgat accgaccatt accaggagtc aattgtcatg
     2821 gattcaatga cacatacgca tgtctgcatc gtcggcgccg ggccgagcgg tctgacactc
     2881 gccaacgaac ttcagcgtta tggtgtgccg ttcgaactga tcgaacgtcg caccgagcgc
     2941 accgtggatt ccaaagcctt tgcgttgcat gcgcgcagcc tggaagtatt ccgccgcatg
     3001 ggcgttgccg atgccatcct tgcccgcggc ctgccggtag cgggcatggc ggcctgggcc
     3061 ggaaagcgcc gcgtactgga aggcgacctg cgcctgctca acagcgaatt tccctgcgtg
     3121 ctcagcattc cgcaggctga taccgaagcg gtgctgctcg acacattgct ggaaagcggt
     3181 ggcactgtgc actgggggca gaccctgacg gcagtgcgcc agaacgcatt gcaggcacaa
     3241 gtggacgtgc gcgacaacga cggcaacacc cgtaccatcg cctgtcgctg gctggtgggt
     3301 tgtgacggcg cccacagcca ggtgcgtcgc ctggccaata tcgactggtc cggcgacgat
     3361 tacgatttcg aattcatggt ggcggacggc aatattgaat ggagcggcga tcttgaatcg
     3421 ggccatactt ttttgaaccg gcagggttac gtgatgttgt tccccttgcc aaaaggacgt
     3481 caccgcatcg tgatcaacgt ggacgtgggc cgtttcagcc gcgaagaatt gtcggccgaa
     3541 ctggtgaacc ggattctgca ggaccgcggc atgggcgatg tgacattcag caatccgttc
     3601 tggctgtcga gcacccaggg tcgccgccgc attgcgggca gtttcaggag cggaagggtg
     3661 ttgctcgcgg gcgatgccgc ccatgtccac agtccactgg gcgggcaggg gctcaacacc
     3721 ggcattcagg atgcctatca actggcgtgg cggctggcag ccatccgcaa ttgcgtggat
     3781 gccgaacatc cgctgctgtc agcttacgcg ctagagcgcc gtcaggttgc acaggaggtg
     3841 ttgaagaata ccgacaagct gacccgtctg ttcaccgcac gggcgccact ggcactgctg
     3901 gtgcgcaatt atgtgattcc gttgctgtca cggcggcgcg cattcaatat ctcgcttgcc
     3961 caaaccgcct ccggcgtggg catccggctg gcgcaacctg cgctggcatt gcgcggcccg
     4021 gtgaatgcaa agctggcggg aacccgactg cttcacgcca gtatcaatga gctgtattcg
     4081 gcaacaccgg tgctgatggg caacgtcatc gcccgcgacg cttttgagct ggtgctgctg
     4141 ttgggcaaag cgcagactcc cgccttttat gaagccaaac gctgtctggc tcaactgcgc
     4201 aacgcggtcc cgcaccccgc ggatcagcac cggaatgccg ttgccatcga accccgtcgc
     4261 agcctgatcg tgacacccgc catcgaaccc atgctggcca cctggccgga agacctgact
     4321 gacacgcgct atctggatgt aagcggtgcg gtactgcagc actacggtat caacgatgct
     4381 ggctacctgc tggtgcgtcc cgacggctac atcgcctgtg gggggccgct gttgaatgcg
     4441 gaggatctca tccacaacct tggcgcagta ctgcactggc catccacaag cgctaccagc
     4501 ttcccacaac ctattgcgac gccgcaccag ctggcggagg tgtaacatga aattcattgg
     4561 cgattttatt gtcttccgcc cgatcacaac cctgctgatc atgttcctgc tgtcggcagc
     4621 ggccatctcc ggtgtcacca aactggaaac ccgcaacaac caggattctg aattaccgga
     4681 caccgatccc atcgtggcca ccaaacacaa catcgacgcc attttcggcg acaagggcgt
     4741 ggtggtgatc ggcatcgagg cggacaacgt ctataacccg tcgacgctgc agaaaatcaa
     4801 ggacatctcc gaagagctga agtctgtcca gtatgtactg ccggaccaga tcgccagcct
     4861 ggcgtcggcc aacaacgtga aatcgcggga ctgggggctg gatgtggggc cgttcatgcg
     4921 cgacgtgccc accacgccgg aagaaatcga gcaactgaaa gccgacgttc gcgccaacac
     4981 gctggtggac ggccggctgg tgacccgtga tggtaccttc accctgattt ctgccaacgt
     5041 cgaagaggat tacgaccagg ccaccgtgtt cgatcaggtg tacaagatcg tcgagaaata
     5101 ccagggcccg gaaaaaatct acgccaatgg cgatccgatt ttcacccagg aaatcgagct
     5161 gggcctgcag cgggatgccg atgtactggt gcccattgcg ctgttaatca tcatgattgg
     5221 cctgtatttc tttttccgca ccctgcgcgg tgtatttctg ccggcgctgg tgatcgtgct
     5281 ctgcatcggc tggatcatgg gattgatggg gcatctgggc ctgccgcaaa cggtggtgtc
     5341 caccgcgatc ccgttgttga tggtggcgat cgcaacatcg tacggcgtgc acgcgctgct
     5401 gatgtattac gaagaaacgg tcggccagga tcagaaaacg gccgtgcgca acatgctggc
     5461 caagctgttt ccggtgttga gtttcgtcgc cttcaccacc ttgctgggta catcgtcgct
     5521 ggtggtgttc gatgtgttgt cgatccgcga gtttgcggtg gaagtcatga tcggcaccgg
     5581 cgttgcgtat ctgatgaccg tgaccgtgat tccggcggca ctgtcactga tgaaggcgcc
     5641 caccaaagtg cccaccgtgg tgcaggaaaa ctggctggac ggtgcgctgg tacggctgac
     5701 tgaattttcc gtgcgtcaca agtataaggt gctgggtggc tacggtgtgc tggtggctat
     5761 ctgtgtcgtg ggtatcgtgc agctgaaaac cggcgtcgat gtcactgccg tgtttccgtc
     5821 ggatcatcgc ggccgcgtat cgttcgacaa gttcagcgaa aaactcgggg gcggccgcta
     5881 tttcaacgtg atggtggaag cacccgacgc ggaaggcatc aaggaccccg cgctgctgaa
     5941 aaccatcctg gattaccagc agtttatcga acagcaggcc ggcgtgggct atacctattc
     6001 gtttgccaac gtggtacggc acatctccca tctggtgaac gagaattaca gcggcaacga
     6061 tattccggct acccgcgagg aactggcgca gtgcctgctg ctgtatgaca tgtcggcgca
     6121 gccgggcgag ttcaccgatc tggtggacaa ccagtaccag cgcgccaaaa tccgggtgat
     6181 gctcgacacc agcgatccgg acgaccacat cagactgtat gaaatgtcgc gggcctacct
     6241 ggagcagcac ctgccggaca acgtgaagat cgagttcggc ggtgacacca tgttctggat
     6301 cgcccaggta cgctacgtgg tgattggcaa gatcgaaaac atcatctccg gcattctgct
     6361 gatcgtgttg ttcgttaccg tgatcagccg ctcctttgtc gccggcatga tcagtatctc
     6421 gcccgccgta attggcagtg tgctggcgtt cggcgtgatg ggctttagcg gtatccgtct
     6481 ggaaatatcc gccgcgctga tcaccagtat cgccatcggt attggcaccg atttctcgat
     6541 tcactacctg gggcgcctgc gggaattcat gcccacttcc gcttccatcg acgaggcatt
     6601 ggtgaaagtg tcgcgcagca acggcaaggc catcgtgtac gacacggcgt ccaacgtgct
     6661 gggttttgcg gtgctgctgt tgtccggctt cctgcagatc cgcaccttcg gcagcctggt
     6721 ttccctcacc atgattgtga tggccgccgg cacgttgatg ctgattcccg cgctggtcgc
     6781 cgccatccag cctgcctatc tgtacaagcg gcgcgaagtg tccgagccac cggtgacacc
     6841 ggctcctgca ctgagtgcgg agtaatgcca tgctgacctc atttacaact gcataccttt
     6901 catcttcatc cgatttgcgc accaaaagga gtaacaccat gttcaagtac aatcgtattg
     6961 ccgccgcact gctggccgga ttcgtcgcca ccgccgccca ggccagtctg gaaatggatc
     7021 cgcgcgaaat catgagaaag aacgaagacg cccgtaaatc cctgaatgaa tcggcgcaga
     7081 tgaaaatgac gctgctggac aaatccaaca acgcccggta ccgctggatt gatttcattg
     7141 ccgacgatac cgatccgttc aatcgcaagt cgctgatccg cttctcgcaa cccaacgaca
     7201 tcaagggcac cggcttcctg tcgatcgaac aaggcaatga ggaggacgac ctgcgttggc
     7261 tctacctgcc gtcgctgcgc aagatccgcc gcatctccgc aggcaacaag accgacagtt
     7321 tcatgggcac cgactggtgg tatgaggatt tcgaattcgt tgatggtgtc gccaaaggcg
     7381 gcaacaacaa ctacaagatc ctgcgcgagg aggaaatgaa cggtgcccgc tgctgggtga
     7441 tcgaagcgat ccccaccacc gagcaggaaa tcaaggaaag cggttacagc aagcgtctga
     7501 tgtgggtgcg ccaggacaat tacgtgctga cccgcgagga aaaattcgac aaaaaaggcg
     7561 agctggccaa aatccagatt gctaccgaga tcaagcagat tcccggcgag gaaaatgcct
     7621 ggcgtgcgca ccggctggaa atggtgacgg tgaaaaccgg ccacaagacg ctgatcgaat
     7681 actcttcgta tcaggccaac gttgcattgc ctgaaggtgc attcagtacg cgttatctgg
     7741 agcaggcccg ctgatgcggg cccatcttga atctgcacgc tcattgttga aaaaggaatg
     7801 atttcatgag tacgacccgc cttgctgtca cgggcctgtt gctgatgcca tacgccgcga
     7861 tggcggccga ctacgagttc cgcacccgct tcgaattcga ggcggcgcaa aacgtcgcgg
     7921 aaggttacgc gcagaaaatg gaattccagg cgcgaccgga gctgcatgtg tttcccgacg
     7981 cggacagtga aataaccgcc attgcccggt tacgggccga tgctgaagac cggctggaag
     8041 tgggccgtcc gctgcaggag gaagtcagcg agtacagccg ccgcgcgatc atcggcagtc
     8101 acggcgatgc cgaattgcgt gagctgtatt acaaacaccg cttcggccag agctatctta
     8161 cggtcggcaa acagcaggtg gtctggggca cggcagacgg cttgcgggta ctggacgtag
     8221 tcaatccgtt cagttttcgc gaattcctgc tggaggattt cgacgaggcg cgcattccgc
     8281 tgtggatggt gaattacatc cactccatcg gatcggacac ggatctgcaa ctgctggtga
     8341 ttcccgatct gaccttcaat gattttcccg acggcgatgg cgacgacgca ccggaatatg
     8401 aaattacctc gccgcgtttc cagccggggt ttcccgccga cgttccaccc aatctggccg
     8461 gcgtgaccaa tctggggctg cagcgcccgt ctgattccct gcagaacgcc gatgcgggcc
     8521 tgcgcctgtc cacccggctg ggcgaactgg atctgacgtt caactatctc tatcagtacg
     8581 acgacaatcc ggtaccgtac cgtcgcatca ataccgccag cggccagccg gtgctggaat
     8641 tctggcagga atatgaacgt acccatctgc tgggttccac gctcagcacg gatctgagca
     8701 gcacgctggt gctgcgctat gaaatcggtc tggtgcacgg ccgccgcgtg ctgcgggaaa
     8761 atctgaacaa tgataacggc gtgctgaaca cggatgaact cagttatgtg ctgggtctgg
     8821 actggagcgg tgtgtccgac accctgttca gcgtgcagct gtttcagagc gtgctgaagg
     8881 atgatgcgcc ggatctggtg cgcgacaggg tggacagcac catgaccttc aacgtcaaca
     8941 gccgtttttt caacgacacg ctgacactgg aattcatggc gctgcacaac ctgaatgatc
     9001 acgacgacct gatccgcccc agcgtcactt atgctgtacg ggacggtgtg aacctatggc
     9061 tgggcgccga tatctttacc ggcgaagagg cgggcgtatt cggccagttc ggcgagaccg
     9121 accgggttct ggccggggtg gagtggtact ggtaagccta ctgcagcacc agatcggcgt
     9181 tttccagacg aaaccggttc aggctttcaa gcggattgcg tccaaacagc ttgcggtatt
     9241 catccgacac gcggcccatg tggcgcagcc cgctgtaaac ggccgccgcc gtgactgtgc
     9301 catgccggcg tagctcgcgg tggaagcggc tgagccggta gcgcttgagg aattgcagtg
     9361 gcgacatgcc gaactgggcc ttgaagacga tctgcaggtt gcgggtggtg gtgtgcacgt
     9421 gattcacgat atcggcaatg cagatgtcgc gcgcgtaatg gcggcggatg aaatccagcg
     9481 cctgggtcag gatacggtcg cggttgctga tgcggggttc gccgggctgg gtgatggtca
     9541 acggagagta ggccagccgg atcaggctgg tcagccctgt atcgacaggt ccggcaacgg
     9601 gcgtcagtga tgggcaaagg gcttgcaggt gtgcgagcag ggccgcgctc accgccagca
     9661 ggcgttgtcc gtccagctgc ctgcgcctca gggtgcgcgt ttgctgctgc agcgattcca
     9721 gttcctctgc ccggaaaatg gattccagta tctgtgtgcg ggtgaccagc accaggcagg
     9781 gctgtccggc tgtcatatgc cagacgggtt catcgccagg cagacccagc agcgaatggc
     9841 gatagccctc aggatgggcc tgaaaggccg tcaaggcgtc gctggcaggg tggggcagat
     9901 gcagcaccca atgaccgctg ggcacgaccc attcctggat ggaaggctga ctgttgtggc
     9961 gccagagcag atcgaagcct tcgcccctca ggacaagcg
//
LOCUS       DASUEG010000123         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_259344, whole genome shotgun sequence.
ACCESSION   DASUEG010000123 DASUEG010000000
VERSION     DASUEG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259344"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..708)
                     /gene="sfsA"
                     /locus_tag="VFX11_02255"
     CDS             complement(4..708)
                     /gene="sfsA"
                     /locus_tag="VFX11_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX4937466.1"
                     /translation="MRFDPPLQSGVLIQRYKRFLADVQLDSGEQITLHCPNTGSMKNC
                     AEPGWRVWFSDSGNAQRKYRHTWELVQMAEGAMVGVNTGRANALVKEAISACAIPSLQ
                     GYDSIRSEVKYGSENSRIDLLLERPGATCHVEVKSVTLGDGRIGYFPDAVTERGRKHL
                     RELMVMAEKGNRAVLFFCVQHTGIECVRPADHIDPAYGKQLREAAESGVEIMAWRWQL
                     SQRECRIEVPLPVQLD"
     gene            complement(705..1892)
                     /locus_tag="VFX11_02260"
     CDS             complement(705..1892)
                     /locus_tag="VFX11_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4937467.1"
                     /translation="MATLVPLAKRLNQIKSFHVMALLDRAKQLESQGRDIIHLEVGEP
                     DFPTPDNVVQAGIRAMSAGQMRYTAPTGLPELKRAIADFYRARYGVVLDWRRVVVTPG
                     ASGAISLVTALLFNPGDGVLLADPGYPCNDNFLHLLGAQPQRVATGPESRYQLTAAQV
                     RHHWRDNTRGAWVASPSNPTGTLLSLAELGAIQAEVDSRSGALLVDEIYHGLTYGLEA
                     ETALALPDRSNSLFVINSFSKYFNMTGWRLGWLVAPERYVEPLDRMAQNFFLAPPTVA
                     QHAALEAFSPQSIALYEQRREILHQRRDFLVRELPALGIRLPCVPQGAFYLYCDVSAI
                     TRDSFAFCGQVLEQTGVAITPGIDFGDHEANHHVRIAYTADLDRLAQAVARLRDFIAR
                     GGA"
     gene            2225..2671
                     /gene="dksA"
                     /locus_tag="VFX11_02265"
     CDS             2225..2671
                     /gene="dksA"
                     /locus_tag="VFX11_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX4937468.1"
                     /translation="MATRKKKNVVELSRSQFTPYEPAKGEEYMSKGQLEHFKAILLRW
                     RQELMEEVDRTVHHMKDESVSLPDAADRATQEEEFSLELRTRDRERKLIRKITQALDK
                     IEKEEYGWCDACGIEIGIRRLEARPTANLCIDCKTLEEIKEKQWGT"
     gene            2883..>3508
                     /locus_tag="VFX11_02270"
     CDS             2883..>3508
                     /locus_tag="VFX11_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4937469.1"
                     /translation="MHLNITTSQKQRIVFVINSLEGGGAERVLSILLDQLAPALPHAD
                     LYLILLDQATERYSIPPSINKITLDCRGSMPASMLQLWQTLRRIAPDVVMSFLNRANC
                     ANIIAARLLGYRCIVSERVNTSSHFGNSLKGRLNKAVVRWLYPLADQVLVVSKGVGSE
                     LERNFGVPAHRLHVIYNPYDLPKLAALAAETPALQIEGDYIVSVGRLVP"
BASE COUNT          746 a         1136 c          976 g          650 t
ORIGIN      
        1 tgttcagtcc agctgcaccg gcagcggtac ctcgatccgg cattcccgct gcgacaactg
       61 ccagcgccac gccatgatct caactcctga ctctgccgct tcccgcaact gtttgccata
      121 cgctggatca atatgatcag caggtcgcac gcactcgatg ccagtgtgct gcacgcagaa
      181 aaacaacacc gcgcggttgc ccttctctgc catgaccatc agctcccgca ggtgcttgcg
      241 gccccgctca gtaacggcat cgggaaaata accgatgcga ccgtccccaa gggttacgct
      301 cttgacctcg acatggcagg tagcgcccgg acgttccagc agcagatcga tacggctgtt
      361 ttcactgccg tatttcactt cggaacggat cgaatcataa ccctgcagcg atggaatcgc
      421 gcaggcggaa attgcctctt tcaccagcgc attggcacgc ccagtattca cacccaccat
      481 cgcgccttct gccatttgca ccagctccca ggtatggcgg tatttgcgct gggcattgcc
      541 gctatccgag aaccacaccc gccagcccgg ctcggcgcaa tttttcatgg agccggtatt
      601 cgggcagtgc agcgtgatct gctcgccact gtccagctgc acatccgcca gaaaccgctt
      661 gtaacgctgg atcagcacac ccgactgcag aggcggatca aacctcatgc gccaccccgc
      721 gcgatgaaat cccgcaaccg cgccactgcc tgcgccagcc ggtccaggtc ggcggtatag
      781 gcaatgcgta catggtgatt ggcttcgtgg tcgccgaaat cgattccggg cgtgatcgcc
      841 acaccggtct gctccagcac ctggccgcaa aacgcaaagc tgtcgcgggt aatagcggac
      901 acatcgcaat acaggtagaa cgccccctgc ggcacacagg gcaacctgat ccccagggcc
      961 ggcaactccc gcaccagaaa atcccgacgc tggtgcagga tctcgcgccg ctgttcgtac
     1021 agggcaatgc tctgcgggga aaaagcctcc agcgccgcat gctgggccac tgtcggcggc
     1081 gccaggaaga agttctgcgc catacggtcc agcggctcga catagcgttc aggcgccacc
     1141 agccagccca gacgccagcc ggtcatgttg aaatacttgg agaagctgtt gatcacaaac
     1201 aggctgttac tccggtccgg cagcgccagg gccgtctccg cctccaggcc gtaggtcagg
     1261 ccgtggtaga tctcatccac cagcagggcg ccgctgcggg aatccacttc cgcctggatc
     1321 gcccccagtt ctgccagcga caataacgtg cccgtgggat tggacggcga agccacccag
     1381 gcaccccgtg tgttgtcgcg ccagtggtgc cgcacctggg ccgccgtcag ctggtaacgg
     1441 gattccggcc ccgtggccac ccgctgcggt tgcgcaccca aaaggtgcag aaagttgtca
     1501 ttgcagggat acccagggtc cgccagcagc acgccgtcgc ccggattgaa cagcagcgcc
     1561 gtcaccaggc tgatggcgcc cgacgccccc ggtgtcacca ccacccgccg ccagtccagc
     1621 acgaccccgt agcgggcccg gtaaaaatcc gcgatcgccc gcttcagttc cggcaacccg
     1681 gtgggcgcgg tatagcgcat ttgccccgcg ctcatggcgc gaattcccgc ctgcaccacg
     1741 ttgtccggcg tgggaaaatc cggctccccc acctccaggt gaatgatgtc gcggccctgg
     1801 gattccagct gtttggcccg gtccagcagc gccattacat gaaaggattt gatctggtta
     1861 agccgtttcg cgaggggtac caaggttgcc atccttctgt catctgattt tcacacaagt
     1921 ggtaaaagat cacggaccgc cagtattcca tagccccgta aatggggtta tgatcgggcg
     1981 ttcgctgccg gcagggcctg tccgcaaagc ccggccaccg atgatataac agcctgttat
     2041 ataactcccg caacggctgg ttcggcgcag cctaacgggg taacacgcgg ttcccggggc
     2101 aggcagcagt gacggcaaat gcggagcggc gttcggggaa cggctccgga gcactactgg
     2161 aatttgccaa tcttgcaatt tatgttatat gaataaagat tgagcagttg cgaggataat
     2221 gcttatggca acacgtaaaa agaagaacgt cgtcgagctt tcgcgctccc agttcacgcc
     2281 gtatgaaccg gccaaaggcg aagagtacat gagcaagggc cagctggagc acttcaaggc
     2341 catcctgctc agatggcgcc aggagctgat ggaagaagtg gaccgcaccg tacaccacat
     2401 gaaagacgaa tctgtcagcc tgccggacgc cgccgaccgc gccacccagg aagaagaatt
     2461 cagcctcgaa ctccgcaccc gcgaccgcga acgcaaactc atccgcaaga tcacccaggc
     2521 actcgacaaa atcgaaaagg aagaatacgg ctggtgtgac gcctgcggca tcgagatcgg
     2581 cattcgccgc ctggaagccc gcccaactgc caatctctgc atcgactgca agaccctgga
     2641 agaaatcaaa gagaaacagt ggggaacctg attcaccact gcgtcatcgc aatactcagg
     2701 cacgtcatca tgacgtgcct tttttgtttt cacccaccgc tctccttcag tcatccttct
     2761 gtcataactt tgtgaccgtt tgcgcaagca tgaatgaccg ccgtggccgg tgcggtcagt
     2821 atcaggcatg atacgtgctt gcctgacact gaccgcaccg ggctacagga atctggaaag
     2881 gaatgcacct caatatcacc acctcgcaga aacagcgtat cgtgtttgtc atcaactcac
     2941 tcgaaggtgg cggtgccgag cgggtactga gcatcctgct ggatcagttg gcgccagcgc
     3001 tgccccatgc cgatctgtac ctgatcctgc tggaccaggc cactgaacgt tactccattc
     3061 caccttcgat caataaaatt acgctggatt gccgcggctc gatgccggcc agcatgcttc
     3121 agctctggca aaccctgcgc cgcatcgctc cggatgtggt gatgagtttt ctcaaccgcg
     3181 ccaactgcgc caatatcatc gccgcgcgcc tgctcggcta ccgctgcatc gtcagcgagc
     3241 gcgtcaacac gtcaagccat ttcggcaaca gcctgaaagg gcgactcaac aaagcggtcg
     3301 tgcgctggct gtatccactg gcagaccagg tgctggtggt gtccaaaggc gtcggctccg
     3361 agctggagcg caacttcggc gtgccggcgc accgtctgca cgtgatctac aacccctacg
     3421 atctgccgaa gctggcggca ttggcggcag agacgccggc actgcagatc gagggcgact
     3481 atattgtgag tgtcggccgg ctggtgcc
//
LOCUS       DASUEG010000124         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2049233, whole genome shotgun sequence.
ACCESSION   DASUEG010000124 DASUEG010000000
VERSION     DASUEG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049233"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1268
                     /locus_tag="VFX11_02275"
     CDS             162..1268
                     /locus_tag="VFX11_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4937470.1"
                     /translation="MIKPFKPYAPEQPWLPFSEVLLDWDEDFHQLMLCDHIRMVAYES
                     AIRSQVRPGQVVVDLGTGTGILALWALQAGAGRVYGIDLDADVLELATQRIHAHGFAD
                     RFEAVNRLSYDVVLPEPADVLISEIIGNMGDNEDFQPILQDAIARLLKPGGTVIPEAV
                     ETFIVPVEAIRTHQDIGNGAVATLNGRYQFQQLLRQKNIRNPFNLYYDAIIPSQQHLA
                     DAQQVKRYDGHWQQAPQYSRALEFAVTATGTLTGFKGFFRARLAPQVILDISSGDIGG
                     RKCSDSWKHCFFPIEQPLAVEQGDRIELRFERRYPHDQAPFRQIYSWHGRVVPRNTAV
                     DAARERPLHFRQSMDESALAGISLPAPDSVPDAG"
     gene            complement(1228..1914)
                     /locus_tag="VFX11_02280"
     CDS             complement(1228..1914)
                     /locus_tag="VFX11_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4937471.1"
                     /translation="MPHPELVLISHHLCPYVQRSVIVLAEKGIAHERRYIDLSDKPDW
                     FLELSPTGKVPLLWVDEQSVLFESAVICEYLNDITPGNLHPDDPLLRAQERAWIEFGS
                     QILNDIARLYNAREEQDFNDQCRLLARKWRRLEMEVQAPYFRGGQFGMVDAAYGPIFR
                     YFDVMDSLLPLDLFDGCDGIRAWRRQLQQRRSVQQAVESSYPERLRQFLRNRNSCISQ
                     RLVQNLVLAG"
     gene            2070..>2376
                     /locus_tag="VFX11_02285"
     CDS             2070..>2376
                     /locus_tag="VFX11_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4937472.1"
                     /translation="MKKLDWSDLYYFAAVARHGSLAKAAQALGVTHSTVFRRITGLEE
                     DLSAKLFDRLPEGYRLTALGRELENYSERIAGTIDEMQRIFENRNEELKGPINLTAPH
                     "
BASE COUNT          495 a          752 c          689 g          440 t
ORIGIN      
        1 tgtttctggc gttcatgtcg ctgagcctgg tgtttattgg cgaaggtgaa tatgcgctga
       61 aaccggaagg cgcaacgcag cccgatctga gtgcctatct gcgcaatccc ggcgccagtc
      121 actcctgatc ctttcactcc atgcaccgca ggtacccggc catgatcaaa cccttcaaac
      181 cctacgcacc ggaacaaccc tggttgccgt tttctgaagt gctgctggac tgggacgagg
      241 atttccacca gctgatgctg tgcgatcaca tccgcatggt ggcgtacgaa agcgccatcc
      301 gttcgcaggt gcggccgggg caggtcgtgg tggatctggg cacgggtacc ggcattctgg
      361 cactgtgggc gctgcaggcc ggcgcaggcc gcgtgtacgg catcgatctg gatgccgacg
      421 tgctggagct ggccacgcag cgcatccacg cccacggttt tgccgatcgc tttgaagccg
      481 tcaaccgcct gtcatacgat gtggtgctgc cggaaccggc agatgtactg atctcggaaa
      541 tcatcggcaa catgggcgac aacgaagatt ttcagcccat cctgcaggac gccatcgccc
      601 ggctgctgaa gccgggcggt acggtgattc ccgaagcggt ggaaaccttc atcgtgccgg
      661 tggaagccat ccgcacccac caggatatcg gcaacggcgc tgtcgccacg ctcaacgggc
      721 gctaccagtt ccagcaactg ctgcgccaga aaaatatccg caatccgttc aacctgtatt
      781 acgacgccat cattccctcg caacagcatc tggccgatgc gcagcaggtg aagcgttacg
      841 acggccactg gcagcaggcg ccgcaataca gccgcgccct ggaatttgcc gtcaccgcca
      901 ccggcaccct gaccggattc aagggatttt tccgtgcccg gctggcgccg caggtgatcc
      961 tggatatttc ctccggcgac atcggcggcc gcaaatgttc cgacagctgg aagcactgct
     1021 ttttccccat tgaacaaccg ctggcggtgg aacagggaga ccgcatcgaa ctgcgattcg
     1081 aacgccgcta tcctcacgac caggcaccgt ttcgccagat ctacagctgg cacggccggg
     1141 ttgttccgcg caacaccgcg gtcgacgctg cgcgcgaacg gcccctccac ttccggcaaa
     1201 gcatggatga atccgccctg gcaggcatca gcctgccagc accagattct gtaccagacg
     1261 ctggctgatg cagctgttgc ggttgcgcag gaactggcgc agccgctccg gataggacga
     1321 ctccaccgcc tgctgcacgc tgcggcgttg ctgcaattgc cgccgccagg cccggatacc
     1381 gtcgcagcca tcaaacagat ccagcggcag cagggaatcc atcacatcga aataacggaa
     1441 aatgggtcca taggcggcat ccaccatgcc gaactgcccg ccgcgaaaat acggcgcctg
     1501 cacttccatt tccagccgtc gccacttgcg cgccagcagc cggcactgat cgttgaaatc
     1561 ctgttcttcc cgggcgttgt acagacgcgc gatgtcattc aatatctggg aaccgaattc
     1621 tatccaggca cgctcctgtg ccctgagcag gggatcgtcc ggatgcagat tgccgggcgt
     1681 gatgtcgttc aggtattcgc agatcaccgc cgattcgaac agcacgctct gctcgtccac
     1741 ccacagcagt ggcaccttgc cggtgggcga cagttcgaga aaccagtcgg gcttgtcgct
     1801 cagatcgata tagcggcgct cgtgggcaat gcctttctca gcgagcacga tgactgagcg
     1861 ttgtacgtag ggacacagat gatggctgat caaaaccagc tccggatgcg gcatgggact
     1921 acctccttgc ggttgttgct ggaacggcgg ctgccgggga tgagaacacc agcagacaca
     1981 tcagccaaca atagcgccac ggctggcgac ggccagcgcg ttttccgcaa accgcatttg
     2041 cgggaaatcg gtaacgagga aactgcaaca tgaaaaaact ggactggagt gatctgtatt
     2101 atttcgccgc cgtggcccgc cacggttcac tggccaaggc tgcgcaggcg ctcggcgtga
     2161 cccactccac cgtattccgc cgcatcaccg ggctggagga agacctgtcg gccaaactgt
     2221 tcgaccggct gccggaaggc tatcgcctga ctgcgctggg gcgcgaactg gaaaactatt
     2281 cggaacgcat cgccggcacc atcgatgaaa tgcagcggat attcgaaaat cgcaacgagg
     2341 aactgaaagg cccgatcaat ctcaccgcgc cgcaca
//
LOCUS       DASUEG010000125         5943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1496532, whole genome shotgun sequence.
ACCESSION   DASUEG010000125 DASUEG010000000
VERSION     DASUEG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496532"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..3248)
                     /gene="carB"
                     /locus_tag="VFX11_02290"
     CDS             complement(27..3248)
                     /gene="carB"
                     /locus_tag="VFX11_02290"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX4937473.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPAMADATYIEPVEWKTVAKVIEKERPDALLPTMGGQTALNCALDL
                     ARHGVLEEFGVELIGATQDAIDKAEDRERFDKAMKSIGLACPKSSIAHSMEEALQVCD
                     MLGFPCIIRPSFTLGGSGGGIAYNREEFMEICERGLDLSPTNELLIDESLIGWKEYEM
                     EVVRDKKDNCIIVCAIENFDPMGVHTGDSITVAPAQTLTDKEYQILRDASLAVLREIG
                     VETGGSNVQFGMCPETGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAIGYTLDE
                     LQNDITGGRTPASFEPSIDYVVTKIPRFNFEKFPKAKPVLTTQMKSVGEVMAIGRTFQ
                     ESIQKALRGLEVGTSGFDPFLDPAAEDFQDQLIHHLKTPGPNRIWVLGDAFRAGLTIE
                     EIHEYTNIDPWYLVQIRDLINDEATLQKSALGDLTKDRLWRLKRKGFSDVRLAKLLGV
                     KEKQVRKRRQELGVKPAYKRVDTCAAEFATTTAYMYSTYEEECEAAPSTRDKIMVLGG
                     GPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLAIVEIEKPKGVIVQYGGQTPLKLARDLEAAGVPIIGTSPDAIDRAEDRERFQQMIE
                     RLGLKQPPNRTARSTEDAVRFAEEIGYPLVVRPSYVLGGRAMEIVYKESELRRYMREA
                     VQVSNDAPVLLDRFLDDAIEVDLDAVSDGQQVVIGGLMQHIEQAGVHSGDSACSLPPY
                     SLGPEIQDQMREQARKMAMELGVIGLMNVQFAIKNNEVYVLEVNPRASRTVPFVSKCI
                     GQSLAKVAARCMAGQTLEAQNFTREIVPKHFFVKEAVFPFAKFQGVDPILGPEMKSTG
                     EVMGSGDTFGEAFGKAALGAGERYPTKGKAFISVREGDKPAAVKVAQQLHDLGFKLVA
                     TGGTSRLIRDAGLPVESINKVKEGRPHVVDAIKNREIDLIINTTEGTQAVADSYEIRR
                     EALQGKISYTTTIAGAQAICAALSVKVEKNVRRLQDLHAELAK"
     gene            complement(3333..4466)
                     /gene="carA"
                     /locus_tag="VFX11_02295"
     CDS             complement(3333..4466)
                     /gene="carA"
                     /locus_tag="VFX11_02295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX4937474.1"
                     /translation="MSKPAILALEDGTLFRGVSIGADGQSVGEVVFNTAMTGYQEILT
                     DPSYARQMVTLTYPHIGNTGVNDEDEESPHIWAAGLIIRDLPLLMSSWRAQKTLDQYL
                     RENNILGIADIDTRRLTRILREMGAQNGCLMAGDNIDEAAAVAAAKGFAGLNGMDLAK
                     EVTVQKAYRWDEGSWQWGVGHKAAPKERPFKVVAYDYGVKRNILRMLVDRGCDLTVVP
                     AQTPAKDVLAMNPDGIFLSNGPGDPAPCDYAINAIRSFLETDIPVFGICLGHQLLALA
                     SGARTIKMKFGHHGANHPVQNLDDKTVLITSQNHGFAVEETTLPANLRATHRSLFDGS
                     LQGIHRTDKPAFSFQGHPEASPGPRDASPLFDHFIELMTARKR"
     gene            complement(4881..5693)
                     /gene="dapB"
                     /locus_tag="VFX11_02300"
     CDS             complement(4881..5693)
                     /gene="dapB"
                     /locus_tag="VFX11_02300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX4937475.1"
                     /translation="MTTNVRVAVIGAAGRMGRALIQAVTEAEGVALTAAIDRPGNSLI
                     GADAGELAGIGKLGVKIADSLDAVKNDFDVLIDFTAPAATVANARFCAAHGKKMAIGT
                     TGLNDEQKAELKICAQKTAIMFAPNMSVGVNLCFKLLEIAAKVLGDEVDIEVLEAHHR
                     HKVDSPSGTALRMGEVVADALGRNLKEVAVYGREGQDGPRKRETIGFATVRAGDIVGE
                     HTVMFAADGERVEITHKATSRMNFARGAVRACRWLNDREAGLFDMQDVLGLR"
     gene            complement(5760..>5943)
                     /locus_tag="VFX11_02305"
     CDS             complement(5760..>5943)
                     /locus_tag="VFX11_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262384.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX4937476.1"
                     /translation="GGNPGDLLCRVVIETPVNLNKEQKELLRQFQESLEKEGSRQSPK
                     RNGWFDGVKSFFDDML"
BASE COUNT         1024 a         1788 c         1843 g         1288 t
ORIGIN      
        1 gccgccgacg gtcatgggta ctcgaatcat ttcgctaact ccgcatgcag atcctgcaat
       61 cgtctgacgt tcttttcgac cttcacactc agtgcggcac agatggcctg ggcaccggcg
      121 atggtggtgg tgtacgagat cttgccctgc agcgcctcgc gccggatttc ataggaatcc
      181 gcgaccgcct gggtgccttc ggtggtgttg atgatcaggt cgatctcgcg gttcttgatg
      241 gcatccacca cgtgggggcg gccttccttc accttgttga tggattccac cggcaggccg
      301 gcatcacgga tcagacgcga ggttccgccc gtggccacca gcttgaagcc caggtcatgc
      361 agctgctgcg caaccttcac ggcggccggc ttgtcgccct cgcggacgct gatgaatgcc
      421 ttgcccttgg tggggtagcg ctcgccggca cccagtgcgg ccttgccgaa ggcttcgccg
      481 aaggtgtcgc cagagcccat gacttcgccg gtggatttca tttccgggcc caggatcgga
      541 tcgacgccct ggaatttcgc gaacgggaat accgcttcct tcacaaagaa atgtttcggc
      601 acgatttccc tggtgaagtt ttgcgcttcc agtgtctggc ccgccatgca gcgcgccgcc
      661 actttcgcca gcgactgacc gatgcacttg gacacgaacg gcacggtacg cgacgcacgc
      721 ggattcactt ccagcacata gacttcgttg ttcttgatgg cgaactgcac gttcatcaga
      781 ccgatgacac ccagttccat ggccatcttg cgcgcctgtt cccgcatttg atcctgaatt
      841 tccggaccca gactgtaggg cggcaatgag caggcggaat caccggagtg cacgcccgcc
      901 tgttcgatat gctgcatcag accgccgatc accacctgct ggccgtcgct cacggcatcc
      961 agatccactt cgatggcgtc gtcgaggaat cggtcgagca gtaccggcgc gtcgttggaa
     1021 acctgcacgg cttcgcgcat gtagcggcgc agttccgatt ccttgtacac gatttccatc
     1081 gcgcggccac ccagtacgta ggacggacgc acgaccagcg gataaccgat ttcctcggcg
     1141 aaacgcacgg cgtcttcggt gctgcgggcg gtgcggttgg gcggctgctt caggcccagg
     1201 cgctcgatca tctgctggaa acgctcgcgg tcttcggcgc ggtcgatggc gtcggggctg
     1261 gtgccgatga tgggcacgcc ggccgcttcc agatcacggg ccagtttcag cggcgtctga
     1321 ccgccgtact gcacgatcac gcctttgggt ttttcaatct cgacgatggc gagcacgtct
     1381 tccagtgtca ccggttcgaa atagaggcgg tccgacgtgt cgtaatcggt ggacacggtt
     1441 tcggggttgc agttgaccat gatggtctcg taaccgtctt cccgcatcgc cagcgccgca
     1501 tgcacgcagc agtaatcgaa ttcgatgccc tggccgatac ggttgggacc accacccagc
     1561 accatgattt tgtcacgggt gctgggtgct gcctcgcact cttcctcgta agtggagtac
     1621 atgtaggcgg tggtggtcgc aaactcggcg gcgcaggtgt cgacgcgctt gtaggcgggc
     1681 ttcacgccca gttcctggcg acgcttgcgc acctgctttt ctttcacgcc cagcagcttg
     1741 gccagacgca cgtcggagaa ccccttgcgc ttcaggcgcc acaggcggtc tttggtcaga
     1801 tcgcccagcg ccgatttctg cagcgtggct tcgtcattga tcaggtcacg gatctgcacc
     1861 agataccagg gatcgatgtt ggtgtattcg tggatttcct cgatggtcag accggcgcgg
     1921 aacgcatcgc ccaacaccca gatccggttc gggccggggg ttttcaggtg atggatcagc
     1981 tggtcctgga aatcttccgc cgccgggtcc aggaaaggat cgaaaccgga ggtgcccact
     2041 tccagcccgc gcagggcttt ctgtatcgat tcctggaacg tgcgaccaat cgccatgact
     2101 tcacccacgg atttcatctg ggtggtgagc accggcttgg ccttggggaa tttctcgaaa
     2161 ttgaaacgcg gaatcttggt gaccacgtaa tcaatggacg gctcgaacga cgccggagtt
     2221 ctaccgccgg taatgtcgtt ctgcagttca tccagcgtgt aaccgattgc cagtttcgct
     2281 gcgacttttg caatcgggaa gccggtcgct ttcgatgcca gcgcggagga acgcgacacg
     2341 cgcggattca tctcgattac gacaagacgg ccggtttccg ggcacatgcc gaactgcaca
     2401 ttggagccgc cggtttccac gccgatttcg cgcagtaccg ccagcgaggc gtcgcgcagg
     2461 atctggtatt ccttgtcggt cagggtttgc gccggcgcca cggtgatgga gtcgccggtg
     2521 tgcacgccca tcgggtcgaa gttttcaatc gcgcagacga tgatgcagtt atctttcttg
     2581 tcccgcacca cttccatttc atattctttc cagccgatca gcgactcgtc gatcagcaac
     2641 tcattggtgg gcgacagatc caggccgcgc tcgcagattt ccatgaactc ttcgcggttg
     2701 taggcgatgc cgccgccgga accgcccagc gtgaacgagg gccggatgat gcaggggaaa
     2761 cccagcatgt cgcacacctg cagcgcttct tccatgctgt gggcgatgga ggatttcgga
     2821 catgcgagac cgatggattt catcgccttg tcgaaacgtt cgcggtcttc cgccttgtcg
     2881 atggcatcct gggtggcgcc gatcagttcc acgccgaatt cttccagcac gccgtgccgg
     2941 gccaggtcga gggcgcagtt cagcgcggtc tggccgccca tggtgggcag cagcgcgtcg
     3001 gggcgctctt tctcgatcac cttggcaacg gttttccact ccaccggttc gatataggtg
     3061 gcgtccgcca tggcaggatc ggtcatgatg gtcgcggggt tggagttcac cagaatcacc
     3121 cggtaaccct cttcccgcag ggccttgcag gcctgtgcgc cggagtagtc gaattcgcac
     3181 gcctggccga tcacgatcgg acccgcgccc agaatgagaa tgctcttgat gtcggtacgt
     3241 tttggcatgt gtgctccggt ggtcacgctg ggcgccgatc cggcgccgca cgtttcaaac
     3301 tcttaaacct tgttgctatc ccgtctgccg tatcagcgct tgcgggctgt catcagctcg
     3361 atgaaatgat cgaacagcgg actggcatcg cgcgggccgg ggctggcttc cgggtgaccc
     3421 tggaaactga aggccggctt gtcggtgcgg tgaataccct gcagcgagcc gtcgaacagc
     3481 gaccggtgcg tggcacgcag gttcgccggc agggtggttt cctccaccgc gaaaccgtgg
     3541 ttctggctgg tgatcagcac cgtcttgtca tccaggttct gcaccggatg gttggcgccg
     3601 tggtggccaa acttcatctt gatggtgcgg gcaccactgg ccagcgccag cagctggtga
     3661 cccaggcaga tgccgaatac gggtatgtcg gtttccagga acgacctgat cgcattgatg
     3721 gcgtaatcac agggcgccgg atcacccggg ccgttggaca ggaagatgcc atcgggattc
     3781 atggccagca cgtctttggc cggggtttgc gcgggcacca cagtgaggtc gcaaccgcgg
     3841 tccaccagca tgcgcaggat gttgcgcttg acgccgtaat cgtacgccac caccttgaac
     3901 gggcgctctt ttggcgcggc cttgtggccc acgccccact gccaggaacc ttcgtcccag
     3961 cggtaggctt tctgcaccgt cacttccttc gccagatcca tgccgttgag tccggcaaaa
     4021 cctttggctg cggcgacggc agcggcttca tcgatattgt caccggccat caggcagccg
     4081 ttctgggcgc ccatttcccg caggatccgg gtgaggcgac gggtgtcgat gtcggcgata
     4141 ccaagaatat tgttctcgcg caggtactgg tcgagggtct tctgcgcccg ccagctgctc
     4201 atcagcaggg gcaggtcacg gatgatcagg cccgccgccc agatatgggg cgactcttcg
     4261 tcttcatcgt tgacgccggt attgccgatg tggggatagg tcagcgtcac catctggcgc
     4321 gcataggaag ggtcagtcag gatttcctga tagccggtca tggcggtatt gaataccact
     4381 tcaccaacgg attgcccgtc ggcgccgatg gacacacccc ggaaaagggt gccgtcttcg
     4441 agggcgagga tggcgggttt gctcaagaca acctccagtg atcgtgcttt atttctgcgg
     4501 gcgccagttg caaaaaagcg gaacggcccc tgtagctcca tcccgctttt ttgtgatggc
     4561 tgctacccgg tggcagcgac cacccggacg acaacagcca acttgtacgt gcgattcttt
     4621 gcgacattcc gacgcttttt ccaacgccct gattttgata cggttttggc cggcaacctg
     4681 ggcgccaact tggcaccggg gcgacaaaaa ctccgggccg aacagaagcg ggctttatac
     4741 agttggactg ccaagccgtg agtactgtct gtcagaggat ttgcgggtag gttgaatccg
     4801 gccccgaccg gggcgctgat cggggcgtat tctataagat tgacccgggc aggtccacgc
     4861 ctttgtggac ccgggtcaga tcagcgcagt cccagtacgt cctgcatatc gaacagaccg
     4921 gcttcgcgat cgttcagcca gcggcaggcg cgcacggcac cgcgagcaaa attcatgcgg
     4981 ctggtggcct tgtgggtgat ttcgacgcgc tcgccgtcgg cggcgaacat gacggtgtgc
     5041 tcgcccacga tgtcgccggc gcgcacggtg gcgaagccga tggtttcgcg tttgcggggg
     5101 ccgtcctggc cttcgcggcc gtacacggcg acttccttca ggttgcggcc gagggcgtcg
     5161 gccaccactt cgcccatgcg cagggcggtg ccggagggag agtccacctt gtggcggtgg
     5221 tgggcttcaa ggacttcgat gtccacttcg tcacccagta ctttggcggc aatttccagc
     5281 agcttgaagc agaggttgac gccgacgctc atgttggggg cgaacatgat ggcggttttt
     5341 tgcgcgcata ttttcagctc ggctttctgt tcgtcgttca ggccggtggt gccgatagcc
     5401 attttcttgc cgtgggcagc gcagaaccgg gcgttggcaa cggtggcggc cggcgcagtg
     5461 aagtcgatga gaacgtcgaa gtcgtttttg acagcgtcga gggaatcggc gattttcacg
     5521 cccagtttgc cgatgccggc cagttcacca gcgtcggcac cgatcaggga gttgccgggg
     5581 cggtcgatgg cagcagtgag ggccacgccc tcggcttcgg tgacagcctg gatgagtgcg
     5641 cggcccatgc ggccggcggc gccgataacg gcaacacgta cgttggtggt cacggggttt
     5701 ccttcgttat tccggaatgg ttttggagtg atcctggcgt ccgtagcgct ggacttcaat
     5761 cacagcatat cgtcaaaaaa cgacttcacg ccatcaaacc agccattgcg cttcggtgac
     5821 tggcgtgagc cttccttctc caggctttcc tggaactggc gcagcagttc cttctgttcc
     5881 ttgttcaggt tcaccggggt ttcaatcacc acacggcaca gcagatcgcc cggattgcca
     5941 ccg
//
LOCUS       DASUEG010000126         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_701323, whole genome shotgun sequence.
ACCESSION   DASUEG010000126 DASUEG010000000
VERSION     DASUEG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701323"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..>2070)
                     /locus_tag="VFX11_02310"
     CDS             complement(161..>2070)
                     /locus_tag="VFX11_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4937477.1"
                     /translation="DDAIVVVEAVEYHMKVNGLSPRDATVRAMPVVSGPVVAIALVLC
                     AVFVPVALLGGISGVMFRQFGLTVAVSVLLSALVALTLTPALCALLLRPHVAHARGLM
                     AGFFAAFNRGFDWLNARYGSGVALALRRSLLMMLTLAALVLATAELFRQVPTTFVPNE
                     DQGYFLASVQLPEASTLARTKDVLAQVETVVRAIPGVEKTLGISGVNLLQSTPQSHAG
                     LLVVRLKQWDERTTEEMSLRNILLTFNREVQAVKEAQVMPFNPPPIPGLSSTGGFSMM
                     LQDKGGRGAAALQQVADDFVAAARQRPEIGMAFSKLQSSTPSVRLEVDRDKAKQLGVS
                     LSDLFATLQTMLGGTQINDFSRFGRTYKVTMQAASEFRANTGALNLLYVRGAGGAMVP
                     LSTLVSWEKAGAPQVLKRFNGYLAAEIGGNPATGYSSGEVLTALEELAAGLPDGYGFE
                     WAGLSRQEKESAGQTLPILVLAVLVVFLFLAALYESWAVPFAVLLALPLGVFGAMLAL
                     WVSGAPGSVYTQIGLILVIGLAAKNAILIVEFAKMKREEGASIRDAAIEAARQRLRPI
                     LMTSLAFILGVVPLILATGAGAASRVTLGLAVFGGMLMATVLAIFLVPVLYALIQQLA
                     ERWSGGDRERG"
BASE COUNT          440 a          742 c          560 g          328 t
ORIGIN      
        1 acatagccgc acaatatacc acgcacctct caaacacctg gcgtggtacg gcctccagaa
       61 atttttcagt ttcgcgggtc tgactggaga ccggaatctt catttcagtg aagttgcgac
      121 agatttatgc gacgttgata ttccacagca gacccgcacc tcagccccgt tcccggtcgc
      181 ccccactcca gcgctctgcc agttgctgga tcagcgcata cagcaccggc accaggaaga
      241 tggccagcac tgtcgccatc aacatgccgc caaacaccgc cagccccagc gtaacgcgcg
      301 acgccgcacc agcaccggtg gccagtatca gcggcaccac gcccagaatg aacgccagcg
      361 atgtcatcag gatcggtcgc aggcgctgac gtgcggcctc gattgccgca tcgcggatgc
      421 tggcgccttc ctcacgtttc atcttggcga attccacaat caggatcgcg ttcttcgccg
      481 ccagcccgat caccagaatc aggccgatct gcgtgtacac agaaccgggc gcaccggaaa
      541 cccagagcgc cagcattgcg ccgaacacgc ccagcggcag cgccagcagc actgcgaacg
      601 gcacggccca gctttcatac aacgccgcca ggaacaggaa caccaccagc actgccagca
      661 ccaggatcgg caacgtctgg cctgcacttt ctttctcctg ccgcgaaagg cctgcccatt
      721 cgaaaccgta accatccggc agacccgcag ccagctcctc caatgccgtc agcacttcgc
      781 cggaactgta gccggttgcg ggattgccgc cgatttctgc cgccagatag ccgttgaaac
      841 gtttcaatac ctgcggcgca ccggcttttt cccaactcac cagcgtggag agcggcacca
      901 tggcaccgcc ggcgccacgc acgtacagca gattcagcgc accggtgttg gcgcggaatt
      961 cgctggcggc ctgcatggtg accttgtagg tgcgaccgaa gcgggaaaag tcgttgatct
     1021 gggtaccgcc cagcatggtc tgcaacgtgg caaacagatc cgacagcgac acacccagct
     1081 gtttggcctt gtcgcgatcg acttccagcc gcaccgatgg cgtgctggac tgcagcttcg
     1141 aaaacgccat accgatttcc ggccgctgcc gcgccgccgc cacgaaatca tcggccacct
     1201 gctgcaacgc cgcagcacca cgcccgccct tgtcctgcag catcatgctg aaaccgccgg
     1261 tggatgacag acccggaatg ggcggcggat tgaacggcat cacctgcgct tccttcaccg
     1321 cctgcacttc gcgattgaag gtcagcagga tgttgcgcag cgacatttct tccgtggtgc
     1381 gctcatccca ctgtttcagc cgcaccacca gcaaacccgc gtgggattgc ggcgtgctct
     1441 gcaacaaatt cacgccgctg atgccgaggg ttttctcgac accgggaatg gcccgcacca
     1501 ccgtctccac ctgcgccagc acgtccttgg tgcgggccag ggtggacgct tccggcaact
     1561 gcaccgatgc caggaagtag ccctgatcct cgttcggcac gaaggtagtg ggcacctggc
     1621 ggaacagttc ggcagtggcc agcaccagcg ccgccagcgt cagcatcatc aacaggctgc
     1681 gacgcagggc cagcgccaca ccgctgccgt agcgggcatt aagccagtca aaccccctgt
     1741 tgaaggcagc gaaaaaaccc gccatcagcc cgcgggcgtg ggccacatgg ggccgcagca
     1801 gcagcgcgca caacgcgggc gtcaacgtca gcgccaccag cgccgacagc agcaccgaca
     1861 ccgccacggt caaaccgaac tggcggaaca tcacgccgga aattccgccc agcaacgcca
     1921 ccggcacgaa caccgcgcat aacaccagcg caatcgccac cacaggccct gacaccaccg
     1981 gcatcgcgcg cacggtggca tcacggggcg acagtccatt cactttcatg tgatattcca
     2041 ccgcctccac caccacgatc gcgtcgtcca
//
LOCUS       DASUEG010000127         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2062274, whole genome shotgun sequence.
ACCESSION   DASUEG010000127 DASUEG010000000
VERSION     DASUEG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062274"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFX11_02315"
     CDS             complement(<1..1051)
                     /locus_tag="VFX11_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4937478.1"
                     /translation="MPYYPEIRTLGDIARFHGTQRPDATAFVFEDRASDYRTFNTHTN
                     QIANGLLAEGIPAQSRIAVMGKNSDWYYELLIGNAKADMVTVGINWRLAPPEVAYILN
                     DAEARILFVGPEFIPLYESIRDQLPAIQKVIVLADGDATYPTFAQWRAAQPVQDPDID
                     VQPHAVAVQMYTSGTTGHPKGVQVAHRAFYALNEIAAQADEPEEEWTTWTDRDVSLLA
                     MPNFHIGGTGWALIGMYAGARNVVLSEFTPAACLQAIRDHRISKLFIVPAAMQFVLAD
                     PTCAETDFSSLKYMIYGASPIPLPLLKRAMDVFKCGFIQLYGMTETIGIGTYLPAEDH
                     HPDGNKRMRSAGKTRA"
     gene            complement(1222..>3681)
                     /locus_tag="VFX11_02320"
     CDS             complement(1222..>3681)
                     /locus_tag="VFX11_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4937479.1"
                     /translation="WQGLPLLLQENRLLNQQIGENIAASLSQQFKQAETLSRNLAQLA
                     EVMPYDDMTYRRVLPHLMDLPELKSLIAGGGLWPEPRQFDPALERNSLFWGRDRDGAL
                     QFFDDYNDPAGHGYHREEWYVPARLQPRDRPYWSRSYTDPYSLQPMVTCTTRFSRQGQ
                     FAGVATVDLKLEGVNALLQEKFRDRPGYAFVVDRNNKFIAFPELDLVRTERPGNNGPV
                     TDFSYASELALIKPVFQPVAEQLALLNQRDSSQADQDSDAVQQLAQAIDDGSYQIERQ
                     EAGVIAHNLTGLASDKPLPLAQFTLDDDILLRQPADVVIHQMPHTFWKIVSVFPRSET
                     TRSAMAISRSLTWGTLAGITLTGLLFALFLWRVLLRRLDYMTHQIREAAQQDPSLSLQ
                     RATLVHHRSEDELDLLGHWYNQRTRQLHEALLAARAGTQQLARENADHRATASLLEKT
                     LALQHALLDSTNLIILSLDSGGMIQHCNAGTLKLLGYGEAELIGKTFPHKLILPVHLH
                     VRQRELQQRLGRSVEGFTLFTQAPESRQWTFHRKDGSPLPVLLQISPVLDSNGRAHGW
                     VAVGTDLSGQPDTTAAPATPAPMPATDDNASPSFLGSLSQELRTALTSILGFSRQLQK
                     KIGSTDALDSIERNAHQLLAIANSLLDLSRIDGGQPDLQTSEFNLGELVDEVHTELRP
                     LLEGKPIDYGYHIPKEPVLMEADRRMIRQILHNLLSCAIRATDHGSVDLTLSHGDTGG
                     HVHLIFSDTGRGFSPEMSQRLFQPHTPLEGYTQHKPGNGLDLYLTWKLVLLHQGRISL
                     DSTPGKGTHITVELPLRPAAA"
BASE COUNT          634 a         1040 c         1251 g          756 t
ORIGIN      
        1 cggcgcgggt cttgccggcg gaacgcatgc gtttgttgcc atcaggatgg tgatcttcgg
       61 cgggcaggta ggtgccgatg ccgatggttt ctgtcatgcc gtacagctgg atgaagccgc
      121 atttgaaaac gtccatcgcg cgtttcagta acggcagggg aatcggtgag gcgccgtaga
      181 tcatgtattt caggctggag aaatcggttt cggcgcaagt gggatcggcc agcacgaact
      241 gcatggccgc cgggacgatg aacagtttgg aaatgcggtg gtcgcggatg gcctgcaggc
      301 aggcggcagg ggtgaattcg cttagcacca cattgcgggc gcccgcatac atgccgatca
      361 gtgcccagcc ggtgcctccg atgtggaaat tgggcatggc cagcaggctg acgtcgcgat
      421 cggtccaggt cgtccattcc tcctccggct catcggcctg tgcggcgatt tcattgagcg
      481 cgtaaaacgc cctgtgtgcc acctgcacac ccttgggatg tccggtggtg ccgctggtgt
      541 acatctgcac ggcaacagca tgaggctgca cgtcgatatc cggatcctgg acgggctggg
      601 ctgcgcgcca ctgggcgaag gtgggatagg tcgcgtcacc gtcggccagc acgatgactt
      661 tttggatggc gggcagctgg tccctgatgc tttcgtacag cggaatgaat tccgggccga
      721 caaacaggat tctggcctct gcatcgttga ggatgtaagc cacttccggc ggtgccaggc
      781 gccagttgat gcccacggtg accatgtcgg ccttggcgtt gccgatcagc agttcataat
      841 accagtcgga atttttgccc atcactgcaa ttcgcgactg ggcgggaata ccttctgcga
      901 gcagcccatt tgcgatctgg ttggtgtgcg tattgaacgt ccggtaatcc gaagcgcgat
      961 cctcaaacac gaaggcggtc gcgtcggggc gctgggtgcc atgaaaacgg gcaatgtcgc
     1021 ccagcgttct gatttccggg taatacggca tgattcgttt cctctcgtga cggtgaatcc
     1081 ggtcgttgcg gtgaaacagg tggacgtccg gaattccggt ggcgcctgcg agagtaacgc
     1141 atatcccttg agtagcagta cacaggctcc ggaactgcct gtcgtcaatg gccagtcaga
     1201 cggcccggtt agcgttatcg ttcaggcagc ggcagggcgc aggggcagct caaccgtgat
     1261 atgggtaccc ttgccgggcg tgctgtccag gctgatgcgg ccctgatgca gcagcaccag
     1321 tttccaggtg aggtacagat ccagcccgtt gccaggcttg tgctgggtgt agccctccag
     1381 cggggtgtgc ggctggaaca ggcgctggct catttccggc gagaaaccac gtccggtgtc
     1441 gctgaagatc aggtgcacat ggccgccggt gtcgccgtgg ctgagggtga gatccacgct
     1501 gccatgatcg gtggcgcgaa tggcgcaact gagcaggttg tgcaggatct ggcggatcat
     1561 gcgccggtcc gcttccatca gcaccggttc cttcggaatg tgatagccgt agtcgatggg
     1621 tttgccttcc agcagcggtc gcagttcggt gtgcacctcg tccacaagtt ctcccaggtt
     1681 gaattcactg gtctgcagat cgggctggcc gccatcgata cgggacaggt ccagcaggct
     1741 gttggcgatg gcgagtagct ggtgggcatt gcgttcgatg ctgtcgaggg catcggtgct
     1801 gccgatcttt ttctgcagtt gacgggagaa acccaggata ctggtgaggg cggtgcgcag
     1861 ttcctgcgac aggctgccca ggaatgacgg gctggcattg tcgtcggtgg ctggcatggg
     1921 agcgggcgtc gccggagcgg ccgtggtgtc cggctggccc gacagatccg tacctaccgc
     1981 cacccagcca tgggcgcgac cgttcgaatc cagcaccggg ctgatctgca gcagcacggg
     2041 cagcgggctg ccgtccttgc gatggaaggt ccactgccgt gattccggtg cctgggtgaa
     2101 cagcgtgaaa ccttccaccg accggcccag gcgctgctgc agttcccgct gccgcacatg
     2161 caggtgtact ggcagaatca gcttgtgcgg gaaggttttg ccgatcagtt ccgcttcgcc
     2221 ataacccaac agtttgaggg tgcccgcgtt gcagtgctgg atcatgccgc cgctgtccag
     2281 gctgagaatg atcaggttgg tgctgtcgag cagggcatgt tgcagcgcca gggttttttc
     2341 cagcaggctg gcggtggcgc ggtgatcggc attttcccgt gccagctgtt gcgttcctgc
     2401 gcgcgcagcc agcagcgcct catgcagctg gcgggtgcgc tggttgtacc agtggcccag
     2461 cagatccagt tcgtcctcgc tgcggtggtg cacgagggtg gcgcgctgca gtgacagcga
     2521 ggggtcctgc tgcgcggctt cacggatctg gtgcgtcatg tagtccagcc gtcgcagcag
     2581 cacccgccac aggaacaggg caaacagcag accggtgagc gtgatgccgg ccagcgtgcc
     2641 ccaggtgagg ctgcgactga tggccatggc ggagcgggtg gtttccgaac ggggaaacac
     2701 gctgacgatt ttccagaacg tatgcggcat ctggtgaatg acgacgtcag ccggttgccg
     2761 cagcaggatg tcgtcatcca gagtgaactg ggccagtggc aggggtttgt ccgaggccag
     2821 tccggtaaga ttatgggcga tgacgcccgc ctcctggcgc tcgatctggt agctgccgtc
     2881 gtcgatggcc tgggccagtt gctgcacggc gtcgctatcc tggtcggcct ggctggaatc
     2941 gcgttggttg agcaaggcca gctgttccgc caccggctgg aaaaccggct tgatcagcgc
     3001 cagttcactc gcatagctga aatcggtgac ggggccgttg ttgccagggc gttcggtgcg
     3061 caccagatcc agttcgggaa aggcgatgaa cttgttgttc ctgtccacca caaaggcgta
     3121 ccccggccgg tcgcggaact tttcctgcag cagcgcgttc accccttcca gtttcaggtc
     3181 cacagtggcg acaccggcaa actgcccctg ccggctgaat cgcgtggtgc aggtgaccat
     3241 gggctgcagg gagtaggggt cggtgtagga gcgggaccag tagggcctgt cgcggggttg
     3301 cagccgggca ggcacgtacc attcctcacg gtggtatccg tggccggcgg gatcgttgta
     3361 atcgtcaaag aactgcaatg caccatcgcg gtcgcggccc cagaacaggc tattgcgctc
     3421 gagtgccgga tcgaactggc ggggttcggg ccagagcccg ccgccggcaa tcaggctttt
     3481 cagttccggc agatccatca ggtgaggcag cacacgccgg taggtcatgt catcgtaggg
     3541 catcacctcc gccagctgcg ccagattgcg tgacagggtt tctgcctgct tgaactgctg
     3601 actgagggaa gccgcgatgt tctcgccgat ctgctggttg agcagacgat tttcctgcaa
     3661 cagcagcggc agcccctgcc a
//
LOCUS       DASUEG010000128         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_714583, whole genome shotgun sequence.
ACCESSION   DASUEG010000128 DASUEG010000000
VERSION     DASUEG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714583"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1531)
                     /locus_tag="VFX11_02325"
     CDS             complement(125..1531)
                     /locus_tag="VFX11_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937480.1"
                     /translation="MKKRVPAITFGLMLLTAALSGTVQADTLVDATTQAYFNEILKEC
                     REQLEQWKRLWMIEVSLAFLVLALGAVSAAIQNFSFKSVKIITVICGLVVTVTTGFIN
                     LVGWNDYRTLDKSISKVQSIIRNMERARRDYEMVKDEDKPVPLKTFGALYADFKRLQE
                     PPPDQIASVLESGFGFLSAAYAGEDVPAWIGTVPDDSRNLYFVGIADSATLKDAKASS
                     REHAIQNATRFLAERFTASGDKALDVDDLVPSLTKSAEDVDSYIVFDAKNGTYRYYSL
                     IRINKSVAEADVRLFAVQRGVRAPTSAIKALGDSQRVRDDYTSKQLVQYEALLDKTGA
                     MLTEQEYRTFSEARKLRKEKKDYARSIVMINEVLAAKPEFYLGWYNLALAYAASGDDA
                     AARSAYEKTIQLEPSQPLRDGTVYNAYGHFLLQRNHSCDAVAMFQKAVELDGNNPRAQ
                     NNLEQARHQVQAAGTNCQ"
     gene            complement(1528..2037)
                     /locus_tag="VFX11_02330"
     CDS             complement(1528..2037)
                     /locus_tag="VFX11_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX4937481.1"
                     /translation="MDVEIVQFPQTRVAVLEHRGPPWREHESVRRLVQWRIENAIPSS
                     LRHRSYGLHYNDPRKVAPAEYRVDLCISVEDDVQPNNHGVIAKVIPACRCAKARHFGS
                     REQVEAAAYLYEQWLPASGEQAADLPVIFHYVNVGPQIQEGEMITDVYLPLCLPEDSQ
                     SSIKGDNKK"
     gene            complement(2438..>3676)
                     /locus_tag="VFX11_02335"
     CDS             complement(2438..>3676)
                     /locus_tag="VFX11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX4937482.1"
                     /translation="KYIAFANDGLRGLLIAMRTIPQAAFDASADLSGWIRELTFVGLV
                     GLMDPPRPEAKEAIVQCTQAGIAVKMITGDHKATGAAIAKELGLEGNAITGAELDQLD
                     AAQLAAIIDDTAVFARVNPAHKVEIVRALQSKGHVVAMTGDGVNDAPALKGADIGVAM
                     GITGTAVAKEAATMVLVDDNFATIVHAVRRGRTLYDNILKFVRFQLSTTIGAILTVFF
                     APLAGLPEPFTPIQILWVAIIMDGPPAISLALDAARPGIMSELPRRRAEPILPFSRLA
                     KIIAYGIVMMVGTLTVLYVGLQTGTEQRALTLAFTTFVLFQVFNVFNARNENDSSINP
                     HLFDNRMLWTSLAGVVVLQVVAVHWPPAKAAFGVGGMTPADWGIAIGVAASVLVLEEV
                     RKLVAVLLIRFRSKAISAAK"
BASE COUNT          832 a         1134 c          933 g          777 t
ORIGIN      
        1 catccggttc atcatccccg ccggactcaa ggtaatccat catgccatcc aggatttgtc
       61 ccttcagcac tacaaattct tctggaatat tggctgacac cttaccgacc tcacatggaa
      121 gaagctactg gcaattggtc cctgcggcct gcacctgatg cctggcctgc tcaagattgt
      181 tttgcgccct gggattattg ccatccagct ccaccgcctt ctggaacatg gccacagcat
      241 cgcaggaatg attgcgctgc agcagaaaat gcccatacgc attgtaaaca gtaccgtccc
      301 gcagcggctg ggaaggctcc agctgaatgg tcttttcata ggccgagcgg gcggccgcat
      361 catcgcccga tgccgcatag gccaaagcga gattatacca gcccagataa aattcaggtt
      421 tcgccgccag cacttcattg atcatcacga tggatctggc gtagtccttc ttttcctttc
      481 gcaatttgcg ggcttctgaa aaggtccggt actcctgctc cgtcagcatc gcacccgtct
      541 tgtccagaag cgcttcgtat tgcaccagct gcttcgatgt atagtcatcg cggacacgct
      601 gggaatcacc caacgccttt atcgcgcttg tgggtgcgcg gacgcctcgc tggacagcga
      661 aaagtcgcac gtccgcttca gcgactgatt tattgatcct gatcaggctg taatagcgat
      721 aggttccgtt cttcgcatca aacacaatat agctgtcaac atcttcggcc gacttcgtca
      781 gcgacggcac aagatcatca acgtccagcg ctttgtcacc cgatgcagtg aacctttcgg
      841 ccagaaaacg ggttgcgttc tggatggcgt gctccctgga actcgccttg gcatccttga
      901 gggtggcact gtccgctatg ccgacaaaat acaggtttct ggaatcgtcc ggcacggtac
      961 cgatccacgc gggcacatcc tcccccgcat aggcagcgga cagaaagcca aatccggact
     1021 ccagcacact ggctatctgg tccggtggcg gctcctgcaa tcgcttgaaa tccgcataca
     1081 gtgcgccaaa cgtcttgagc ggtaccggct tgtcttcgtc cttgaccatc tcatagtcac
     1141 gccgtgccct ttccatgttg cggataatgg actgcacctt gctgatggac ttgtccagcg
     1201 tccggtaatc attccagccc accagattga taaacccggt cgtgacggtg accaccaacc
     1261 cgcagatcac ggtgataatc ttcaccgatt tgaaactgaa gttctgaatg gccgccgaca
     1321 ccgctcccag cgccaggacc agaaacgcca gcgacacttc aatcatccac aatcgtttcc
     1381 actgctctag ctgctccctg cattccttca gaatctcatt gaagtacgcc tgggtagtgg
     1441 catccaccaa ggtgtcagcc tgtaccgtgc cggaaagcgc agcggtcaac agcatcagtc
     1501 cgaaagtaat cgctggaacc cgttttttca ttttttattg tctcctttga tgctggattg
     1561 ggaatcttcc ggcaggcaaa gcggcagata gacatctgtg atcatctcgc cttcctgtat
     1621 ctgcgggcca acattgacat agtgaaaaat aaccggaagg tctgccgcct gttcaccact
     1681 ggcgggcaac cattgttcat acagatacgc tgcggcctcc acctgttcgc gcgagccaaa
     1741 atgcctggct ttcgcgcaac ggcaagcggg aatgactttg gcaatcacgc catggttatt
     1801 gggctgaaca tcgtcttcaa ccgaaataca gagatcgacc cggtattctg ccggtgcgac
     1861 cttgcgtggg tcattgtagt gaagcccata gctgcggtgc cggagtgaag acggaattgc
     1921 attctcgatg cgccactgaa ccagtcgcct aaccgattcg tgttcacgcc agggcggccc
     1981 ccggtgctcc aataccgcaa cccgcgtttg cggaaattgc acaatttcaa catccatagc
     2041 ttgtttccaa cgtaagttgc ctgtgctgac tcctgttccg ccccacctta cctgacgtgg
     2101 aaccgactga catgatgcat ggcaaacttt tcaacacacg gattgcccca gctccactac
     2161 tgcgtgccaa cgtccagcag gccaacctgc ggcccgggat ctgcaatccg caggcaggca
     2221 tcgatgaaaa aatccggata taacactgtt tcaaagatgg cactgtttac catgaaacat
     2281 gacgcccttt ccataacagg gctcgtccaa gcgcactacg aacaggatca tggcgcttgt
     2341 acaaaaagcg aacacaaaag tcccagcttc gtcctgactg cctgatccga agttgaaaga
     2401 agcctggccc tgactaccag cgaacagggg actgatgtca ctttgccgca gaaatggcct
     2461 ttgaccggaa ccgtatcaac aacacggcca ccagcttccg cacctcttca agtacaagca
     2521 cagacgcggc cacaccgatg gcgatccccc aatccgcggg cgtcatgcca cccaccccaa
     2581 aggctgcctt tgctggaggc cagtgcacgg ccacaacctg aagtacaaca acacctgcaa
     2641 gcgacgtcca caacatcctg ttgtcgaaca ggtgtgggtt gatcgaggag tcgttctcgt
     2701 tgcgcgcatt gaacacattg aagacctgga acagcacaaa cgtggtgaag gccagcgtca
     2761 gcgcgcgctg ctcggtgcca gtctgcaacc cgacatacag cactgtcagc gtgccgacca
     2821 tcatcacgat cccgtaagcg atgatcttcg ccagccggga aaacggcagg atgggctccg
     2881 cacgacggcg cggcagctca gacatgatgc ccggacgcgc tgcatcaagt gcgagcgaga
     2941 tcgccggagg accgtccata atgattgcaa cccagagaat ctgaatcggc gtgaagggct
     3001 ccggcaagcc ggccagaggc gcgaagaaga ccgtgagaat tgcaccgatg gtggtcgaca
     3061 gctggaagcg gacgaacttc agaatgttat cgtagagcgt acgcccgcgt cgcacagcgt
     3121 gcacaatggt ggcgaagttg tcatcgacca gcaccatggt ggcggcttcc ttggccactg
     3181 ccgtgccggt gattcccatg gccacaccga tatcggcacc cttgagggcg ggcgcatcat
     3241 tgacaccgtc gcccgtcatg gcgacaacgt gtccttttga ttgcagcgca cgcacgatct
     3301 ccactttatg cgccgggttg acgcgggcaa aaaccgccgt gtcatcgata atcgcagcca
     3361 gttgggcggc atccagctga tcaagttccg cccccgtgat cgcgttaccc tcgaggccga
     3421 gctccttcgc aatcgcggcg ccagtcgcct tgtgatcgcc cgtaatcatc ttgaccgcga
     3481 tgcccgcctg cgtgcactgg actattgctt cctttgcctc gggccgtggc gggtccatca
     3541 aacctaccag gccaacgaac gtcagctccc tgatccaccc ggatagatcg gccgaggcat
     3601 caaatgcagc ctgcggaatc gtcctcatag caatcagcag accccgcagc ccatcattcg
     3661 caaacgcaat atactt
//
LOCUS       DASUEG010000129        10311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_945754, whole genome shotgun sequence.
ACCESSION   DASUEG010000129 DASUEG010000000
VERSION     DASUEG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945754"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFX11_02340"
     CDS             complement(<1..351)
                     /locus_tag="VFX11_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937483.1"
                     /translation="MSFVARTIFEEEHLVFRDNFRRFCEEEVAPHGEKWIEQGIVDRE
                     IWKLAGDNGFLAPFVDEEFGGLGLKDFRFSQIMIEELSRIGESGFALSLHNDVIAPYL
                     ESYGTEEQKQRWLPG"
     gene            complement(348..518)
                     /locus_tag="VFX11_02345"
     CDS             complement(348..518)
                     /locus_tag="VFX11_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937484.1"
                     /translation="MSLKMAHYVIVGGNGGTNNEHQSVTPASLLPEPLLKQFDRTPND
                     VAISFGFYGVAK"
     gene            677..1690
                     /locus_tag="VFX11_02350"
     CDS             677..1690
                     /locus_tag="VFX11_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937485.1"
                     /translation="MTGNIIPITQVLLLLEAAKRSGVSSEELLAESGIDPKLLREGEA
                     HVDKRLFIQLMLAVMHRTEDEFMGFGEGRVSKPGTFSMMAHAVINCATLEKAIRRGIK
                     FYELFDLAIVGRLLQTEDEASIRFVSRAEVNFRPYIMEAVVFLTVRFLSWLIGQQIVP
                     KRINLDFSALDDGDYAALFPCQVHYKQSHCEILLDSRYMTMPLVQNALSLSKFLRSSL
                     EELMDGDIKNASLNAQIRSIISKEFGNNFPDFSVVCDKLAMTPQTLRRRLKDENTSYQ
                     EIKDTIRKDASIFYLSKPELTIDEIALLMGFSEASSFHRAFKKWTGKTPSAYRHELLA
                     PDS"
     gene            complement(1701..3365)
                     /locus_tag="VFX11_02355"
     CDS             complement(1701..3365)
                     /locus_tag="VFX11_02355"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEX4937486.1"
                     /translation="MKILVVDDTEANRKLLAWILEDEGHSVIEARDGQQAVDMFRQHE
                     PDLVLMDVMMPVMDGFEATQAIKEHLGGRYVPIIFLTALSDDASLAKCLSIGGDDFLS
                     KPINEQVLQAKIKAHSRIKELNEQLNRKNQELQRLHQITVREHEIAKAVFDNSLAASR
                     SDSGNIRQYISPATTFNGDLLLVSVSPSGGLYALIADFTGHGLPAAIGAIPLSQTFFD
                     ATQQGASVSSISRSLNRALTKFLPDDMFASAAIAELNADGTRLSLWLGGMPDAILIDS
                     QGNLKETIGSNHMPLGILGDEEFERDPFTLEVSRGDRLFLYTDGIPESENPAGEMYGE
                     TRLLSHFNSRRADPFTALIDDVRDFTGGKSQIDDITLLELTCQPVDIALQQSDDQTRH
                     KPALPWHIDMKLSPEELRSGSPVALLIDMLGSTPDMERHKDYLHTILAELFSNALEHG
                     VLALSSDLKKTEDGYLDYYQQREEKLRRLNQGFVNIGVQYAVHEGKGRLLIRLEDSGK
                     GFDFSGRRPSDDDDAFGRGISLLQTLCEKVTYSNGGSRVEVEYRIE"
     gene            complement(3376..3672)
                     /locus_tag="VFX11_02360"
     CDS             complement(3376..3672)
                     /locus_tag="VFX11_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4937487.1"
                     /translation="MPVESALSSDGKTLTISISGRFDFEIVQEFRGAYSQHKGVNYVI
                     DMRATEHMDSSALGMLLNMRKSLGDSTKISIINSRPQIKKILTISRFDKKFDIE"
     gene            complement(3703..4875)
                     /locus_tag="VFX11_02365"
     CDS             complement(3703..4875)
                     /locus_tag="VFX11_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937488.1"
                     /translation="MTKYNLVTGLLAGVAAAVILLNQNPFIEAAAIVLSAIAVVVLRK
                     WESRANTQPVSAELIALAQHPTLQTALNDNLSGCARINRSVDTLGSTMRESSAQLSSS
                     FSGLVSKSNQTNALITSIMQRVTERNHTASQNADDPVTVGKFAEEVSDVLMQYVSLLI
                     DVSEKSIQAVHHIGDMVTELDQMFRLLADIRTIAEQTNLLALNAAIEAARAGESGRGF
                     AVVADEVRRLSKATNALSDQIRNRAETAKSKVTEVRDIVGKIASLDLNDAINAKGHVD
                     QMLQGLEEANQSISATMDQLNALNTAAKQDVSAAVQALQFEDRANQILAELRTDLERI
                     NNINYRMGVLCEVARIDAADKDRIETLQNLLAVKSKDSLLHKRSGSNDSSGEIDLF"
     gene            complement(4994..6916)
                     /locus_tag="VFX11_02370"
     CDS             complement(4994..6916)
                     /locus_tag="VFX11_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4937489.1"
                     /translation="MPLIRLRDLHLAFGGPQLLSGASLTLDKGERICLVGRNGTGKST
                     LLKIISGQIKPDDGVVEVQPGIRIAWLEQEVPQDARGTVFDVVASGLGRVGEYIQRYH
                     HLLDDVAHGDEKAFAELEQVQKQLEAHDGWQMEQRVETALSKLDLKGDVEFLAQSGGV
                     KRRVLLARALVQEPDILLLDEPTNHLDIESIQWLEEFLLQYRNCVLFITHDREFLRKL
                     ATRIVDLDRGKLSDWPGDYDNYLRRKEEVLHAEALEWERFDKKLAQEEVWIRQGIKAR
                     RTRNEGRVRALEAMRNERSQRRERQGNVKASIQQAESSGQKVIEAEHISVAFNDRPIF
                     TDFSTVILRGDRIGIIGPNGVGKSTLIQTLLGQRKPDSGKVTVGTNVEVAYFDQLRAQ
                     LDENMNVRDNVGGGRDSVTINGQQRHIMGYLQDFLFSPDRAHTPIRQLSGGERNRLLL
                     AKLFTKPSNLLVLDEPTNDLDIETLELLEEMVSQYQGTILLVSHDRQFLNNIVTSTFV
                     FEGNGVINEYVGGYDDWLRQRKPVASPQPAKETSSLQGKSKNEAPAPAPASTPASAAK
                     PRKKLSYKEQRELETLPQTIEKFEQEIAALTAQLSDPNLYQPGNSKAADLQSRLAAAE
                     QALEVAFARWAELDSQ"
     gene            complement(6918..9446)
                     /locus_tag="VFX11_02375"
     CDS             complement(6918..9446)
                     /locus_tag="VFX11_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4937490.1"
                     /translation="MLSSAQRNHFALALRMLKRDWQSGHLNVLVMALLVAVSTHATIG
                     FISERIQNSMLVQASALLGGDLVIRSPSPPTAAWFEAAGQRGIRHAQTLEFSSVVLAG
                     DAMQLAAVKAVTDEYPLKTPLKVSDAPFAPEQAVTHGPNPGKVWLEQRMLTALSINVG
                     DTVEVGDATLRVEKVLVFEPDRSGNFYSFVPRVLMHWDDIDATGVIQPGSRLDYYAQF
                     TGDTAAIQTLQDEIKLQLQPDQRILSVHSQQRSISSALQRAEGYLQLASLMAILLAAV
                     AIAMGAHFFSRQHFDTAALLRCFGLSSRQILQLFIIQLIVLALLAGLIGSAIGWSLHF
                     VAVTMLGELLPANTPLPGPQPLFTGILLALVILPGFALPSLTQLQRTPPLRVLRRDLA
                     PQPLSQLVSYGSSALLVALLMLWYARDPVLIAAIFAGAAAGWLVATLLTDLFMRALLV
                     ISRFSPLSVQAGLRNLARRRNGTRIQLLGFGLTGMAMLMVILVRTELISTWEGQLPEQ
                     APNHFVLNVLPEQATAFTQFLHANDVVSQPLYPVVRGRLTHINDNAVKQAVSKEKQQD
                     GEFDAPEALNRELNLTFAPDIPSDNVIVEGNWWNTGVASTEKQISIEQKLAQKLNVQL
                     GDKLTFFVANQTFSARVASIRTVKWESFQPNFYVIFHPGDLDEMPTTYLTSFYLSAHQ
                     KALLKDLVQQFPAVSVLEVDAILARVKGILAQVSAAVEFILLFVLAAGLTITLATLIT
                     TMPERYREGALMRTLGATGRQLRLQQWSEFFAIGFLAGIVAAGGCELARFGVYWKIFN
                     LPYTPALWIWIIMPPLFGVLIGLAGHWSSRKILGQSPLILLRDS"
     gene            complement(9446..10087)
                     /locus_tag="VFX11_02380"
     CDS             complement(9446..10087)
                     /locus_tag="VFX11_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4937491.1"
                     /translation="MAEQPLHILKSVSLSVHAGESLAIVGASGSGKSTLLGLLAGLDQ
                     PSSGSIRMLGHALEKMSEDERARVRAKHVGFVFQSFQLLPNLTALENVMLPLEISNTD
                     RVRERAADMLSAVGLGERMLHYPNQLSGGEQQRVALARAFVCEPDILFADEPTGNLDT
                     QTGARIADLLFGLNETRNTTLVLVTHDHTLAQRCARKLVMNAGQLESTAGSAP"
BASE COUNT         1966 a         2944 c         3134 g         2267 t
ORIGIN      
        1 gcccggcagc cagcgctgct tctgctcttc agtgccgtag gactccaggt agggagcgat
       61 cacgtcgttg tgcaggctca gtgcaaagcc gctttcaccg atgcgggaca gttcctcgat
      121 catgatctgg gaaaaacgaa aatccttgag gcccaggccg ccgaattcct cgtcgacgaa
      181 gggggcgaga aagccgttat cgccggccag tttccagatc tcgcggtcga caatgccttg
      241 ctcgatccat ttctcgccgt ggggagccac ttcctcttcg cagaagcgac ggaagttgtc
      301 acggaatacc aggtgctctt cttcaaaaat ggtgcgggcg acgaaactca ttttgctact
      361 ccgtagaaac caaatgaaat ggcgacatca ttcggcgtgc gatcgaattg ttttagtaac
      421 ggttccggca acaaacttgc cggagtgact gattggtgtt cattattagt gccgccatta
      481 ccaccaacaa tgacgtaatg tgccattttt aatgacataa aacgacaggg cgtatggaag
      541 ttccgggacg aaggcagtag agttacaagc gatttactcc gttgcgcgcg gaacgtacag
      601 tttccatcgt aatgaacatc caggtttctg tcgagcctgc ataacatatt gaaatccaat
      661 gtaaaaggcg tggagtgtga ctggaaacat aattcccatc actcaggttc tgttgctgct
      721 ggaagcagcc aagcgctcgg gcgtcagcag tgaggagctg ctggcagaaa gtggtatcga
      781 tcccaagctg ttgcgggaag gtgaagccca cgttgacaag cggcttttca ttcagctgat
      841 gctggcggtc atgcaccgca ccgaagatga attcatgggg tttggcgagg gacgggtgtc
      901 gaaacccggc accttcagca tgatggcgca tgccgtgatc aattgtgcca ccctggaaaa
      961 agcgattcgc cggggtatca agttctacga actgtttgac ctggcgattg tcggccgttt
     1021 gctgcagacc gaggacgaag ccagcattcg ctttgtgtcc cgcgccgaag tgaatttccg
     1081 gccttacatc atggaagcgg tggtgtttct cactgtgcgc ttcctgtcct ggctgattgg
     1141 ccagcagatc gtgccaaagc ggatcaacct ggatttttcc gcgctggatg atggcgatta
     1201 cgcggcgctg ttcccctgtc aggtgcacta caaacagtcc cactgcgaga ttctgctgga
     1261 cagccgctac atgacgatgc cgctggtgca gaatgcgctg tcgctcagca aattcctgcg
     1321 ctcgtcgctg gaagagctga tggatggcga tatcaagaat gccagcctga acgcgcagat
     1381 ccgcagcatt atcagcaagg aattcggcaa caacttcccg gatttttccg tggtgtgcga
     1441 caagctggcg atgacgccgc aaaccctgcg tcgccggctg aaggatgaaa acaccagcta
     1501 tcaggaaatc aaggacacga tccgcaagga tgcgtcgatc ttctatctgt cgaaaccgga
     1561 attgaccatt gacgagattg cgttgctgat gggattttcc gaggccagtt cctttcaccg
     1621 cgccttcaag aaatggacgg gcaagacgcc gtctgcttac cgtcacgaac tgctggcgcc
     1681 ggattcctga accgccggcg tcactcgatc ctgtattcca cttccacccg gctgccgccg
     1741 ttgctgtagg tgactttttc gcaaagtgtc tgcaacagtg agatgccgcg accgaaagcg
     1801 tcgtcatcgt ctgacgggcg acgcccgctg aaatcgaagc ccttgccgct gtcttcaagc
     1861 cggatcagca gacggccctt gccctcgtgg acagcatact ggacgccaat attgacgaaa
     1921 ccctggttca ggcggcgcag tttttcttcc cgctgctggt aatagtccag gtagccgtcc
     1981 tcggtttttt tcaggtctga gctcagggcc agtaccccgt gttccagcgc attggaaaac
     2041 agttccgcca gaatggtgtg cagataatcc ttgtgccgtt ccatgtccgg ggtggagcca
     2101 agcatgtcga tcagcagagc gacgggcgag ccactgcgca gttcctccgg gctcagcttc
     2161 atgtcgatgt gccagggcag ggcgggcttg tggcgggtct ggtcgtcgct ctgctgcagc
     2221 gcgatatcca cgggctggca ggtaagctcc agcagggtga tgtcatcgat ctggcttttg
     2281 ccgccggtga aatcccgcac gtcgtcaatc agcgcggtaa acggatcagc ccggcggctg
     2341 ttgaaatggc tgagcagacg cgtttcgccg tacatttcgc cggcgggatt ttccgattcc
     2401 ggaatgccgt cggtgtaaag gaagagacgg tcgccccggc tcacttccag cgtaaaaggg
     2461 tcccgttcga attcctcgtc gccgagaatg cccagtggca tgtggttgga accgatggtt
     2521 tccttcaggt tgccctggct gtcgatgaga atggcgtccg gcatgccgcc gagccagagg
     2581 gacaggcggg tgccgtcggc attgagttcg gcgatggcgg ccgaggcaaa catgtcgtcc
     2641 ggcaggaatt tggtcagcgc gcggttgagc gaacgcgaga tgctgctgac agaggcgccc
     2701 tgctgggtgg cgtcgaaaaa ggtctgcgac aggggaatgg cgccaatggc cgccggcagg
     2761 ccgtggccgg tgaaatccgc gatcagcgcg tacagaccac cggaagggct gaccgaaacc
     2821 agcagcagat cgccattgaa ggtggtggcc ggggagatgt actggcggat attgccactg
     2881 tcggagcggc tggcggccag cgaattgtcg aagacggcct tggcgatttc gtgttcccgt
     2941 acggtgatct ggtgcagtcg ctgcaattcc tgattcttgc ggttgagctg ctcgttcagt
     3001 tccttgatgc ggctgtgggc cttgatcttc gcctgcagca cctgttcgtt gatgggcttg
     3061 ctgagaaagt cgtcgccgcc gatcgacagg catttcgcca gtgacgcatc gtcggacagg
     3121 gcggtgagaa aaatgatggg aacgtagcgg ccacccaggt gttccttgat ggcctgggtg
     3181 gcctcgaatc catccatcac cggcatcatc acgtccatca gcaccagatc cggttcgtgc
     3241 tgccggaaca tgtccaccgc ctgctggcca tcgcgggctt cgatgacgct gtggccttcg
     3301 tcttccagaa tccaggccag aagcttgcgg ttggcctcgg tgtcgtccac caccaggatt
     3361 ttcataacgt cggcgtcatt cgatatcgaa tttcttgtcg aagcgggaaa tggtcaggat
     3421 tttcttgatc tggggccggc tgttgatgat cgatattttc gtactgtcgc ccaggctctt
     3481 gcgcatgttc agcagcatgc ccagagcaga actgtccatg tgctcggtgg cgcgcatgtc
     3541 gatgacgtag ttgacgccct tgtgctggga ataggcaccg cggaattcct gcacgatctc
     3601 aaaatcgaag cgtccgctga tactgatggt gagggtcttt ccgtcgctgg aaagtgcgct
     3661 ttcaaccggc attgtatttc tccttgatcg gtttcgggct gttcagaata aatcgatttc
     3721 gcctgagctg tcgttgctgc cggaccgttt gtgcaggagg gaatccttgc ttttcacggc
     3781 cagaagattc tgcaatgttt cgatgcgatc tttgtcggca gcatcgatgc gggcgacctc
     3841 acagagtacg cccatgcggt agttgatatt gttgatccgc tccagatccg ttcgaagttc
     3901 tgccaggatc tggttggccc tgtcttcgaa ctgcaatgcc tgcaccgccg cgctgacgtc
     3961 ctgtttggca gcggtgttga gtgcgttaag ctggtccatg gtagcggaaa tggactggtt
     4021 tgcttcctcc agtccctgca gcatctggtc aacatgcccc ttggcattga tggcatcgtt
     4081 caggtcgagc gaagcgatct tgcccacaat atcacgaact tccgttactt tggactttgc
     4141 tgtttcggcg cggtttcgta tctggtcgct gagggcattg gtggctttcg acagccggcg
     4201 tacttcatcg gcgaccacag caaatccccg ccccgattcg ccggctcggg ccgcttcaat
     4261 ggcggcgttg agtgcgagca ggttggtttg ctcggcgatg gtgcggatat cggccagcag
     4321 ccggaacatc tggtccagtt cggtcaccat atcgccaatg tggtgtacgg cctggatgct
     4381 cttttcgctc acgtcgatca gcagcgacac gtactgcatg agaacatcgc tgacttcttc
     4441 ggcgaatttt ccaacggtga cgggatcgtc cgcattctgg ctggcagtat ggttccgttc
     4501 ggtgaccctt tgcatgatgc tggttatcaa ggcattggtc tggttggact tgctgacaag
     4561 gccggagaaa ctggatgaca attgcgcaga gctttctcgc atggtgctgc ccagtgtgtc
     4621 gacgctgcga ttgatgcggg cgcagccgga gagattgtcg ttcaatgccg tttgcagggt
     4681 gggatgttgc gccagcgcga tcaattctgc actgacaggc tgtgtgttgg cgcggctttc
     4741 ccatttccgc aggacaacaa cagcgatagc agacagcacg atggccgctg cttcaataaa
     4801 tggattctgg ttcagcagga tgacggcggc ggccacccct gccagcaatc cggtcactag
     4861 attgtacttt gtcaaaggga atgatcctgc agacaggcat acgccaggca ataaaattat
     4921 tgcctgtttt gtcatcagtt tagatcagac cgtccctttt tccactgcgt gactcggggt
     4981 ttgggcgggg tggttactgg gaatcgagct cggcccagcg tgcgaatgcc acttccagcg
     5041 cctgttctgc agcggccagt cgggattgca ggtcggcagc cttggaattg ccaggttgat
     5101 acaggttggg atcggacagt tgcgcggtca gcgcggcgat ttcctgctca aatttttcaa
     5161 tggtttgagg cagtgtttcc agttcccgtt gttccttgta actgagtttt ttgcgcggtt
     5221 tggccgcgct ggcgggagtg gatgcgggcg ccggcgcagg tgcttcgttt ttcgatttgc
     5281 cctgcaggct ggacgtttct tttgccggtt gcggtgatgc cacgggcttg cgctggcgca
     5341 gccagtcgtc gtagccaccc acatattcgt tgatcacgcc gttgccttcg aatacgaacg
     5401 tactggtgac gatgttgttg agaaactggc ggtcgtggct gaccagcagg atggtaccct
     5461 ggtactggct caccatttcc tccagcagtt caagggtttc gatgtccaga tcgttggtcg
     5521 gttcgtccag caccagcagg ttggatggct tggtgaacag cttcgccagc agcagacggt
     5581 tgcgttcacc accggacagc tggcggatcg gtgtgtgggc acggtccggg ctgaacagga
     5641 aatcctgcag gtagcccatg atgtgacgtt gctggccgtt gatggtgacg gaatcgcggc
     5701 cgccgccgac gttgtcgcgc acattcatgt tctcgtccag ttgcgcgcgc agctgatcga
     5761 aataggcgac ttccacattg gtgccgacgg tgaccttgcc gctgtccggc ttgcgctggc
     5821 ccagcagggt ctggatcagg gtggatttgc ctacgccgtt ggggccaatg atgccgatgc
     5881 ggtcgccgcg cagaatgacg gtggaaaaat ccgtgaagat gggtctgtcg ttgaaggcga
     5941 cgctgatgtg ttcagcttcg atgactttct gcccgctgga ttccgcctgc tggatgctgg
     6001 ctttcacgtt gccctggcgt tcacggcgct gggagcgttc gttgcgcatg gcttccagtg
     6061 cgcgcacgcg gccctcgttg cgggtgcggc gcgcttttat gccctggcga atccagactt
     6121 cctcctgcgc cagcttcttg tcgaagcgtt cccattccag cgcctctgcg tgcaacactt
     6181 cctctttgcg gcgcaggtag ttgtcgtaat cgccgggcca gtcggagagt ttgccgcgat
     6241 cgaggtcgac gatgcgggtg gccagtttgc gcagaaactc gcggtcgtgg gtgatgaaca
     6301 gaacgcagtt gcggtattgc agcagaaatt cttccagcca ctggatggat tcgatgtcca
     6361 gatggttggt tggttcgtcc agcagcagga tgtcgggctc ctgcaccagc gcgcgggcca
     6421 gcagcacgcg gcgcttcaca ccgccggact gggccaggaa ttccacatca cctttcagat
     6481 ccagtttgga cagcgcggtt tccacccgct gttccatctg ccagccgtcg tgggcttcca
     6541 gctgtttttg gacctgctcc agttcggcga aggctttttc atcgccgtgg gccacgtcgt
     6601 cgagcagatg atggtaacgc tggatatatt cgccaacgcg accgaggcct gatgccacca
     6661 catcgaatac ggtgccgcgc gcatcctgcg gcacttcctg ctccagccac gcgatcctga
     6721 tgcccggttg cacttccacc acgccatcgt ctggcttgat ctggccgctg atgattttca
     6781 gcagcgtgga cttgccggtg ccgttgcggc ccaccagaca gatgcgctcg cccttgtcga
     6841 gggtgaggga ggcgcccgac agcagttgcg ggccgccgaa ggccaggtgc aggtcgcgca
     6901 gtcggatcag gggcatgtca ggaatctcgc agcaggataa gggggctctg gcccaggatt
     6961 ttgcggctgc tccagtggcc ggcgaggccg atcagcacgc caaacagggg cggcattatt
     7021 atccagatcc agagcgccgg ggtatagggc aggttgaaaa tcttccagta cacgccaaac
     7081 cgtgccagtt cgcagccgcc cgctgccacg attcccgcca gaaaaccgat ggcgaagaac
     7141 tcgctccact gctgcagccg tagttgccgg ccggtggcgc ccagggtgcg catcagggcg
     7201 ccttcgcggt agcgctccgg catggtggtg atcagggtgg ccagggtaat ggtgagcccc
     7261 gccgccagca cgaacagcag gatgaattcc accgccgcgc tcacttgcgc caggatgcct
     7321 ttcacccgcg ccagaatcgc atccacttcc agcacgctca cggcggggaa ctgctgcacc
     7381 agatccttca gcaaggcttt ctgatgggcg gaaagataga aactggtgag gtaggtggtg
     7441 ggcatctcat ccagatcacc cggatggaag atgacataga aattcggctg gaaactttcc
     7501 catttcaccg tgcggatgct ggcgacacgg gcgctgaagg tctggttggc aacgaagaac
     7561 gtgagcttgt cgcccagctg cacgttcagc ttctgcgcca gtttctgttc gatggaaatc
     7621 tgcttttcgg tgcttgcgac gcctgtgttc caccagttgc cttccacgat gacgttgtcg
     7681 ctgggaatgt ccggtgcaaa ggtcaggttc agttcgcggt tgagggcctc gggtgcgtcg
     7741 aattcgccgt cctgctgctt ttccttgctc acggcctgtt tcacggcgtt gtcgttgata
     7801 tgggtgagac gaccacgaac cacgggatac agcggctgcg agaccacatc gttcgcgtgc
     7861 aggaattgtg taaatgcagt ggcctgttcg ggcagtacat tcagcacgaa atgattgggt
     7921 gcctgctccg gcaactgacc ttcccaggtg gaaatcagct cggtgcgcac cagaatcacc
     7981 atcagcatgg ccatgccggt aaggccgaaa cccagcagct ggatgcgggt gccgttgcgc
     8041 cgccgcgcca gattgcgcag ccccgcctgc accgacaggg gactgaaccg gctgatcacc
     8101 agcagcgcgc gcatgaacag atccgtcagc agggttgcca ccagccagcc tgccgctgca
     8161 cccgcaaaaa tcgcggcgat cagaaccgga tcgcgggcgt accacagcat cagcagtgcc
     8221 accagcagtg ccgaggaacc gtaactcacc agttgcgaca gcggttgcgg cgccagatcc
     8281 cgccgcaata cccgcagcgg tggcgtgcgc tgcaattgcg tcagcgaggg cagggcgaaa
     8341 cccggcagaa tcaccagcgc cagcagaatt ccggtgaaca ggggctgcgg accgggcagg
     8401 ggagtgttgg ccggcagcag ttcccccagc atcgtcaccg caacgaaatg cagcgaccag
     8461 ccgatagcac tgccgatcag ccccgccagc agtgccagca caatcagctg gatgatgaac
     8521 aattgcagaa tctgtcgcga cgacaaaccg aaacagcgca gcagtgccgc cgtgtcgaaa
     8581 tgctggcgac tgaaaaaatg cgcgcccatg gcaatggcca ctgctgccag caggattgcc
     8641 atcaggctgg ccagttgcag gtagccttcc gcccgctgca gcgccgaact gatgctgcgc
     8701 tgctgggaat gaactgacag aatccgctgg tccggttgca gctgcagctt tatttcgtcc
     8761 tgcaaggtct gaatggcggc ggtgtcgccg gtgaactggg cgtaatagtc cagccggctg
     8821 ccgggctgga tcacgccggt ggcgtcaata tcgtcccagt gcatcagtac gcggggtacg
     8881 aagctgtaaa aattgccgct gcgatccggt tcgaacacca gcactttttc cacgcgcagt
     8941 gtcgcgtcgc cgacttccac cgtatcgccg acatttatgc ttaacgccgt cagcatgcgc
     9001 tgttccagcc acaccttgcc ggggttgggg ccatgtgtca ccgcttgctc cggtgcaaac
     9061 ggcgcgtcgc tcactttcag cggcgttttc agcggatatt cgtcggtcac cgcttttacg
     9121 gctgccagct gcatggcatc gccggccagc accacgctgg aaaactccag tgtctgcgcg
     9181 tgacggatgc cgcgttgccc ggctgcctca aaccaggccg cggtgggtgg cgacggcgag
     9241 cggatcacca gatcgccgcc cagcagcgcc gacgcctgca ccagcatgga attctggatg
     9301 cgctcggaaa taaagccgat ggtggcgtga gtggatacgg ccaccagcag cgccatcacc
     9361 agcacattga gatggccgga ctgccagtcg cgcttcagca tgcgcaatgc cagcgcaaaa
     9421 tgatttcgtt gcgcagatga cagcatcagg gtgcacttcc cgcagtggat tccagctggc
     9481 ctgcgttcat caccagcttg cgcgcgcagc gctgagccag ggtatgatcg tgggtcacca
     9541 gcaccagtgt ggtgttgcgg gtttcattca ggccgaacag cagatcggcg atgcgggcac
     9601 cggtttgcgt atccagattg ccggtgggtt cgtcggcaaa cagaatgtcc ggctcgcaca
     9661 cgaaggcgcg ggccagggcc acccgctgct gttcgccgcc ggacagctgg ttggggtagt
     9721 gcagcatgcg ctcacccagg cccacggcgc tcagcatatc cgctgcccgt tcccgcacgc
     9781 ggtcggtatt gctgatttcc agcggcagca tcacgttttc cagcgcggtg agattgggca
     9841 gcagctggaa cgactgaaac acaaagccca catgctttgc tcgcacccgg gcgcgttcgt
     9901 cctccgacat tttttccagc gcgtgcccca gcatgcggat cgacccgctg ctgggctggt
     9961 ccagccccgc cagcaatccc agcaaagtgg acttgcccga gccggacgcc cccacgatcg
    10021 ccagggattc accggcatgc acgctcaaac tcacggattt cagtatgtgc agcggctgtt
    10081 ccgccatgta cacggttttt tcaatggctt ccgccagcaa gatgggcccg gcagcacgct
    10141 gcgtctgagc attgtgagcc gctgaacttt gcgcccggtt gtcctgtccc atacactgtt
    10201 ccactcctac acagtcaggg agccatccta cccaatgctg aagcgaatgt gcactcttgc
    10261 ctgcattctc ctgctggcta taaccagcaa tcaaatccat gcgcagcaaa a
//
LOCUS       DASUEG010000130         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_620524, whole genome shotgun sequence.
ACCESSION   DASUEG010000130 DASUEG010000000
VERSION     DASUEG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620524"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VFX11_02385"
     CDS             <1..1823
                     /locus_tag="VFX11_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX4937492.1"
                     /translation="DQQAQATPNNIAVKQGSVLFTYAQLQQKSNQLANWLLQTGVQKG
                     QKIGICLDRSPDMLVAVLGALKAGAAYVPMDSEYPPERLAYMLEDSRAPVLLTQKCVV
                     ERLQQGSVNLHGVNVVQLDQQPAYLQAAYLQAPGTTPSVTISGDDPIYVIYTSGSTGK
                     PKGAVVRHAGEVNLQHWYINALNISAQDKFLLISAFGFDLTQKNLFAPLLTGGTLVIP
                     AMENYDVEVIADTLQKEAISIVNCAPSAFYPVVEETARKGYPFSALRYLVLGGEPIRM
                     NALQAWLDHPSCQCKVVNSYGPTECTDVVSYHINQDNAATVLPIGKPVCNTQLYIVGA
                     GNHLLPEGVVGELCVAGNGVGLGYLDRPELTASVFQNNPFARPGSAANNLWYRTGDLA
                     RYWPDGNIEYIGRKDFQIKLRGLRIELGEIEHALRRQDGVSDSLTLVRDDQLVSYVIA
                     PKTFDTQGCRVKLRNALPDYMVPAHILVLERWPLTPNGKVDRNALPSPTDTGRGPYVA
                     PRNATEEKLAAIWSEVLGVDRIGVHDSFFDLGGHSLLAARAVSKFRHTFNVDIPLRAL
                     FELHTIADIAQYLETLQWAAQAAETSQQAGSSEGRDEGFL"
     gene            1961..3742
                     /locus_tag="VFX11_02390"
     CDS             1961..3742
                     /locus_tag="VFX11_02390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937493.1"
                     /translation="MPVSLFRCSLLFLLLLCALGAQADEPAPRIALNSHAWVYLDETG
                     TQNLAQIADPDQLLQKFPFQPTPHGEDNWGYGQPVFWVLVPLDKLGAHNKWFLEFAYP
                     LMDKLDLWLKFPHSEWLHRTTTGDQRPWGTREVESPQFYFQIPRQTTHLLLRLETNGA
                     MRFPLALVSESVLQRDERLLQFGYGLFFGALLVMMFCNAVIYLLAAERSYLFYMALLT
                     AVTLYQAAMSGFGFMYLWPGSGYWINEHIQPITVGLAMWFVCLFTRDVLQLELLRPRL
                     DRLLVWLGWSGALLAVAGILLPFRWLIHISALWPLVVIMTVVHSGIIALRARQKGAGI
                     FVVAWSCGFLGAALFILQQLGVLAPSWITEHGLKLGLILNMTLLSFVLVSHMQQLRRE
                     KAKLEQTARDNYELALIDGLTGVPNRRAFDDRLLSEFERTSRDGTSMALLMIDIDYFK
                     KFNDAYGHHAGDEALIRVALIMRNCLRRPTDSLYRYGGEEFTVILADTDQQGADHIAT
                     RIMRAIRGLCLPHQDSPYRQITVSIGSSLTTGREDSASQLLQRADAALYRAKRQGRNT
                     CCPGETSSPGAVVNMGDYFKNTPKDTG"
BASE COUNT          725 a         1206 c         1182 g          758 t
ORIGIN      
        1 tcgaccagca ggcacaggcc acgccgaaca atatcgcagt gaagcagggc agtgtgctgt
       61 tcacttacgc ccagctgcag cagaaatcca accagctggc caactggctg ctgcaaaccg
      121 gcgtgcagaa gggccagaag atcggcatct gtctcgaccg ttcgcccgac atgctggtgg
      181 cggtgctcgg tgcgctgaaa gccggcgctg cctacgtgcc gatggattcg gaatatccgc
      241 ccgagcgtct ggcctacatg ctggaggatt ccagggcacc ggtgctgctc acgcagaaat
      301 gcgtggtgga gcggctgcag cagggcagtg tgaatctgca tggcgtcaat gttgtgcaac
      361 tggaccagca gccggcttat ctgcaggcgg cttatctgca ggcgccaggc acaacgccgt
      421 ccgtcaccat cagcggcgac gatccgatct acgtgatcta cacctccggc tccaccggca
      481 agccgaaagg cgcggtggtg cgccatgccg gcgaagtgaa tctgcagcac tggtacatca
      541 acgccctgaa catttctgcg caggacaaat tcctgctgat aagcgcattc ggtttcgacc
      601 tgacccagaa aaacctgttt gcgccgctgc tcaccggcgg caccctggtg attccggcga
      661 tggaaaacta cgacgtggaa gtgattgccg acacgttgca gaaggaggcc atcagcatcg
      721 tcaactgtgc gcccagtgcg ttctacccgg tggtggagga aacggcgcgc aagggttatc
      781 cattctccgc gctgcgttat ctggtgctgg gtggcgaacc gatccgcatg aatgcgctgc
      841 aggcctggct ggatcatccg tcgtgccagt gcaaggtggt gaacagctac ggccccaccg
      901 agtgcaccga cgtggtgtcg taccacatca accaggacaa cgccgccaca gtgctaccca
      961 tcggcaagcc ggtgtgcaat acgcagcttt acattgtcgg cgccggcaat catctgctgc
     1021 cggaaggcgt ggtgggtgaa ctgtgcgtgg cgggcaacgg cgtgggtctg ggctatctgg
     1081 accggcccga actcacggcg tcggtgttcc agaacaatcc gttcgccagg cccggttccg
     1141 ccgcgaataa cctgtggtat cgcaccggcg atctggcccg ttactggccc gatggcaaca
     1201 tcgaatacat cggccgcaag gacttccaga tcaagctgcg gggcctgcgc atcgagctgg
     1261 gtgaaatcga acacgccctg cgccggcagg acggtgtcag cgacagcctg accctggtgc
     1321 gcgacgatca gctggtgtcc tacgtgatcg cacccaaaac gttcgatacc cagggctgcc
     1381 gcgtgaaact gcgcaacgcg ctgccggact acatggtgcc ggcccacatc ctggtgctgg
     1441 agcgctggcc ccttacgccc aatggcaagg tggaccgcaa cgcactgccg tcgcccaccg
     1501 acaccggccg tggcccctac gtggcgccgc gcaacgcgac ggaagaaaaa ctggcggcga
     1561 tctggagtga agtgctgggt gtcgaccgca tcggcgtgca cgacagcttc ttcgacctgg
     1621 gtggccattc cctgctggcg gcgcgggcgg tgtccaagtt ccgtcacacc ttcaatgtcg
     1681 acatcccgct gcgggcgctg ttcgaactgc acaccatcgc cgacatcgcc cagtacctgg
     1741 aaaccctgca gtgggcggcc caggcggcgg aaacctcgca gcaggccggc agcagcgaag
     1801 ggcgggacga gggcttcctc tgatggtccg cagtcagtat tggttcaatc aggcacggca
     1861 gccgctccgg ttgttgccgt gcctccagcc ccccgcacag gccatcatgg cagggatgtg
     1921 ctaggctgga cttctgtccc cagccagtca ggcccaccgg atgccggtct ccctgtttcg
     1981 ctgcagcctg cttttccttc tgctgctgtg cgcccttggt gcacaggcgg acgagcctgc
     2041 gccgcgcatt gccctcaaca gccacgcctg ggtttacctg gatgaaaccg gaacccagaa
     2101 tctggcccag atagccgatc ccgaccaact gctgcaaaaa tttccgtttc agcccacgcc
     2161 ccacggcgag gataactggg gctatggcca gccggtgttc tgggtgctgg tgccgctgga
     2221 caagctcggc gcccataaca agtggtttct cgagttcgcc tatccgctga tggacaagct
     2281 ggatctgtgg ctgaagtttc cccacagcga gtggctgcac cgcaccacaa ccggcgacca
     2341 gcgtccctgg ggcacccgcg aagtggaaag cccccagttc tatttccaga ttccgcgcca
     2401 gaccacccac ctcttgttgc gcctggaaac caacggtgcc atgcgtttcc ctctggcact
     2461 ggtcagcgag tcggtgctgc aacgggatga gcgtctgctg cagttcggct acggcctgtt
     2521 tttcggcgcg ctgctggtga tgatgttctg caatgcggtg atctatttgc tggcggcgga
     2581 acgttcctat ctgttctaca tggcgctgct cacggccgtc accttgtatc aggccgcgat
     2641 gagcggtttc ggtttcatgt acctgtggcc ggggagtggc tattggatca acgagcacat
     2701 ccagccaatc accgtcgggc tggccatgtg gtttgtctgc ctgttcaccc gcgatgtcct
     2761 gcagctggag ctgctgcggc cgcggctcga ccgtctgctg gtgtggctgg gctggagcgg
     2821 tgcgctgctg gcggtggcgg gaatcctgtt gccattccgc tggctgatcc atatctccgc
     2881 gctgtggccg ctggtggtga tcatgacggt ggtgcattcc ggcatcatcg ccctgcgggc
     2941 gcggcagaag ggcgccggta tttttgtggt ggcctggagc tgcggttttc tgggggctgc
     3001 gctgttcatc ctgcagcaac tgggtgtgct ggcgccatcc tggataaccg aacatggttt
     3061 gaaactgggt ctgatcctga acatgacgct gctgtcgttc gtgctggtgt cgcacatgca
     3121 gcaattgcgg cgggaaaagg caaagctgga acagaccgcc cgcgacaatt acgaactggc
     3181 gctgatcgac ggcctcaccg gcgtgcccaa ccggcgtgca ttcgacgacc gcctgctgtc
     3241 cgagtttgaa cggacgtccc gcgatggcac ctccatggcg ctgctgatga tcgatatcga
     3301 ctatttcaag aagttcaacg acgcctacgg ccatcacgcc ggcgatgaag cgctgatccg
     3361 ggtggcgctg atcatgcgca actgcctgcg gcggcccacc gacagcctgt accgttacgg
     3421 tggcgaggaa tttaccgtga ttctggcaga taccgaccag cagggcgccg accatattgc
     3481 cactcgcatc atgcgtgcca tccgcgggct gtgcctgccg caccaggaca gcccctaccg
     3541 gcagatcact gtcagtatcg gttccagtct caccaccggg cgcgaggaca gtgccagcca
     3601 gttgctgcag cgcgccgacg ctgccctcta ccgcgccaag cgccagggcc gcaatacctg
     3661 ctgcccgggc gaaacctcat cgccgggcgc tgtcgtcaat atgggcgact atttcaaaaa
     3721 cacaccaaaa gacaccggct aacccctttc aaaccgttgc tttttgactg aaaattgccg
     3781 gtttgtatct gcttcggaaa gtccgtttca atcgcgtata cactcggtat acattacaag
     3841 aaaaagaaca ggaacattat gaacgtagta g
//
LOCUS       DASUEG010000131         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_418412, whole genome shotgun sequence.
ACCESSION   DASUEG010000131 DASUEG010000000
VERSION     DASUEG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418412"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFX11_02395"
     CDS             complement(<1..749)
                     /locus_tag="VFX11_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4937494.1"
                     /translation="MIKWLLAPLVLLALATGILILLPSPISATAWQPPKAPAMIDVYA
                     PNQLLDAAELLGEGKIDGPEDVDVDSQGRVYGGTADGNIVRVLKDGSVETVATTGGRP
                     LGLHWDANGRLIICDAFKGLFSLDVDTGTLTNLLTEVDGVPLVFTDDLEIARDGMIYF
                     SDASIKYDQKHYMYDMLEARPWGRLIRYNPATGEATTLLRDLYFANGIALSQNNDFVL
                     INETFRYRIIRYWISGPNAGQHDVFIDNLPG"
     gene            962..1037
                     /locus_tag="VFX11_02400"
     tRNA            962..1037
                     /locus_tag="VFX11_02400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:996..998,aa:Glu,seq:ttc)
     gene            1149..1850
                     /gene="nfi"
                     /locus_tag="VFX11_02405"
     CDS             1149..1850
                     /gene="nfi"
                     /locus_tag="VFX11_02405"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953981.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEX4937495.1"
                     /translation="MPSSPVQTKPLHGWDVSPAEARALQSRLAPLVEVQDRLPDVRFI
                     GGVDVGFEHGNRVTRAALVVLSYPDLQIVERQLAKIPTAFPYVPGLLSFREMPAILQA
                     YSRLNQKPDLLLCDGQGIAHPRRLGIASHLGLWLDLPTIGVAKKKLVGQHEAVPDTKG
                     AWVPLQDKGETIGAVLRSRQGVLPLYVSPGHRVSQAGAVDWVLRCLTRYRLPEPTRQA
                     HKLASLEKLSVPEQK"
     gene            complement(1857..2558)
                     /locus_tag="VFX11_02410"
     CDS             complement(1857..2558)
                     /locus_tag="VFX11_02410"
                     /inference="COORDINATES: protein motif:HMM:NF006562.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX4937496.1"
                     /translation="MKKILLIKTGSTHAAIRDQFGDFEHWFLQSLTGCDTQLVNAVEA
                     TTLPACTDIDGVVITGSPAMVTDREAWSERLAEWLATQAHGQVPILGVCYGHQLLAHA
                     LGGAVDWHPQGREIGTTPIHLTDAGTRDPLLGTLPARFLAQVTHAQRVSRLPDEAVLL
                     AGNDYESHHAFRVGDATWGLQFHPEFSAEVMRAYLHASAAALRQHQRDPDQLLQQVDT
                     AHADQALLRRFVELL"
     gene            2638..>3350
                     /locus_tag="VFX11_02415"
     CDS             2638..>3350
                     /locus_tag="VFX11_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4937497.1"
                     /translation="MHDRMYGADARAQTDWKVIYRLLPYLWEFRLRVIVAMSFLIIAK
                     LANVAVPVLLKHIVDALDKSRQPEVTTLIAIPLALLLAYGLLRFSSVLFGELRDAVFG
                     LVAERSLTRIGLVVFRHLHKLDLEYHLSRRTGGLSRDIERGTTGVSFLLRSLVFSVIP
                     ILVEVAMVIGIFIWQFDAGFAWITLGGIVVYVAFSVSVTNWRTEFVRTANLKDSQANT
                     KAVDSLLNFETVKYFTNEE"
BASE COUNT          612 a          929 c         1094 g          715 t
ORIGIN      
        1 aagccgggca ggttgtcgat gaacacgtcg tgctggcccg cgttggggcc actgatccag
       61 tagcggatga tgcggtagcg gaaggtttcg ttgatcagca cgaagtcgtt gttctgcgac
      121 agggcgatgc cgttggcgaa gtacagatcc cgcagcaggg tggtggcctc gccggtggcc
      181 gggttgtagc ggatcagccg gccccaggga cgggcttcca gcatgtcgta catgtagtgt
      241 ttctggtcgt acttgatgct ggcatcgctg aaatagatca tgccgtcgcg ggcgatttcc
      301 agatcgtcgg tgaagaccag gggaacgccg tccacttcag tgagcaggtt ggtgagggtg
      361 ccggtgtcca cgtccaggga aaataatccc ttgaaggcat cgcagatgat caggcgaccg
      421 ttggcgtccc agtgcagtcc cagtgggcgt ccgccggtgg tggcgacggt ttccacgctg
      481 ccgtccttca atacgcgcac aatgttgccg tcagctgtgc cgccatagac ccggccctgg
      541 ctgtccacat ccacatcttc cgggccgtcg atcttgcctt caccgagcag ctccgcggcg
      601 tccagcaact ggttgggcgc gtagacatcg atcattgcag gcgccttggg aggctgccag
      661 gccgtggcac tgatgggcga gggcagcagg atcaggatgc cggtggcaag agcaagcaga
      721 acaagggggg cgagcagcca tttgatcatt gttattttcg tccttggcgt gtggggcgcg
      781 ggcttgtgca gcccgtgagg ggtgtcatgc taggggaatc ggcacgcaaa tgaaacctgc
      841 tgatccgcca ccaggttgtt gatatgtgac agaaagcaga tcgggccgga aaaaattctt
      901 gacgcaattt ttgccactcc atagaatgcg cgcctcgaca cgatgtcggc cgtttcattg
      961 cgtccccttc gtctagtggc ctaggacact gccctttcac ggcagtaaca ggggttcgaa
     1021 ccccctaggg gacgccacta ttctcatgtg caaactcata cgaaaaaagc aggcttgatg
     1081 cctgcttttt ttgtttctga tgccgtccga taatccgggt tctgcccgat tcagccggag
     1141 cccgccgcat gccttcatct cctgttcaaa ccaaacccct gcatggctgg gatgtctcgc
     1201 ccgccgaggc ccgcgcgttg caaagccggc tggcgccgct ggttgaagtg caggatcgtc
     1261 tgcccgacgt gcgttttatc ggcggagtgg atgtgggatt cgagcatggc aaccgcgtca
     1321 cccgcgccgc gctggtagtg ctgtcgtatc cggacttgca gattgtcgag cggcagctgg
     1381 caaaaattcc cacggcgttt ccctatgtgc ccggcctgct gtcgttccgc gagatgccgg
     1441 cgattctgca ggcgtattcc cgtttgaatc aaaaaccgga tttgctgttg tgcgatgggc
     1501 aaggtattgc ccatccgcgc cggcttggta tcgcgagtca tctgggattg tggctggatc
     1561 tgccgaccat tggcgtcgcc aagaaaaagc tggtgggaca acatgaagcg gtgcccgata
     1621 caaaaggtgc ctgggtgccg ctgcaggaca agggtgagac gattggcgcg gtgttgcggt
     1681 cgcggcaggg tgtgctgccc ttgtatgtgt cgccagggca tcgggtgagt caggccggcg
     1741 cggtggactg ggtgctgcgt tgtctgacgc gctaccgtct gccggagccg acgcggcagg
     1801 cgcacaagct ggcgtcgctg gaaaaactgt ctgtgccgga acaaaagtag cggcggttac
     1861 agcagttcca cgaaacgccg cagcagtgcc tgatcggcgt gggcggtgtc cacctgctgc
     1921 agcaattgat ctggatcgcg ctggtgctgg cgcagggcag cggcgctggc gtgcaggtag
     1981 gcccgcatca cctccgcgct gaattcgggg tgaaactgca gaccccaggt ggcgtcgccg
     2041 acgcgaaagg cgtgatggga ttcgtagtca tttcccgcca gcaggacagc ttcatcgggc
     2101 aggcggctga ccctttgcgc atgggtgacc tgggccagaa agcgggcggg cagggtgccc
     2161 agcaggggat ctcgcgtgcc ggcgtcggtg aggtgaatgg gcgtggtgcc gatctcgcgg
     2221 ccctgcggat gccagtccac cgcgccaccc agcgcgtgcg ccagcagttg atgtccgtag
     2281 cagaccccca gaatgggcac ctgtccgtgc gcctgcgtgg caagccattc cgccagccgt
     2341 tcgctccagg cttcgcgatc cgtcaccatg gccggcgagc cggtgatgac gacgccgtcg
     2401 atgtcagtac aggccggcag cgtcgtggct tccaccgcat tcaccagttg ggtatcgcac
     2461 cctgtcaaac tctgcagaaa ccagtgttcg aaatcgccga actggtcgcg gatggcggca
     2521 tgggtggaac cggtcttgat cagcaggatt tttttcatgc gggtgaatcg atccggaatg
     2581 cgggcggatg gattatgctt gtctgaaaac ggtataacaa aatcaacggg agaacaaatg
     2641 cacgatcgca tgtacggcgc tgacgcccgg gcccagaccg actggaaagt gatttaccgg
     2701 ttattgccgt atctgtggga attccgcctg cgggtcattg tggccatgag ctttctgatc
     2761 attgccaagc tggccaacgt ggcggtgccg gtgctgctga aacacatcgt cgacgcactc
     2821 gacaagagcc ggcagcccga ggtaaccacg ctgattgcga ttcccctggc actgttgctg
     2881 gcctatggtc tgctgcgttt cagcagcgta ctgttcggcg aactgcgcga tgcagtattc
     2941 ggactggtgg ctgagcgcag cctgacgcgg atcgggctgg tggtgttccg tcatctgcac
     3001 aaactggatc tggaatatca cctcagccgg cgcaccggcg gcctgtcgcg ggatatcgag
     3061 cgtggtacca caggcgtgtc ttttctgctg cgttcgctgg tgttcagcgt gattccgatt
     3121 ctggtggaag tggcgatggt gatcggcatt ttcatctggc agttcgatgc cggattcgcc
     3181 tggatcacgc tgggcggtat tgtggtgtac gtcgcctttt ccgtgtccgt taccaactgg
     3241 cggaccgagt tcgtgcgcac tgccaatttg aaagacagcc aggccaatac caaggctgtg
     3301 gacagcctgc tgaatttcga aaccgtgaaa tattttacca acgaggaatt
//
LOCUS       DASUEG010000132         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_427247, whole genome shotgun sequence.
ACCESSION   DASUEG010000132 DASUEG010000000
VERSION     DASUEG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427247"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFX11_02420"
     CDS             complement(<1..301)
                     /locus_tag="VFX11_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937498.1"
                     /translation="MSETRPSHLADSELPIPAPYFELYGRFWGVASLLIFSLAAVGVL
                     IARANELTWREWAVAGLSIAQGLLYFFCITRGGWPVSARNLVLYFVGGIGMWALAC"
     gene            complement(411..1139)
                     /locus_tag="VFX11_02425"
     CDS             complement(411..1139)
                     /locus_tag="VFX11_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4937499.1"
                     /translation="MKRFLILTKAIFLMHLRNGFVLFWNFAFPVLLMLIYGVVMSQYM
                     DYMTPGVIVLNALSFGLVGSSNMMLEMREKSVLRRLQASPLPAGQLISSYLLVNLLIG
                     ILQSTLIIIAGVLLYKVPVSALSLALAYPMILAGLLAFMALGGIISGVAAKAGTATAV
                     GMTIYSSLMFISDLVFPLEMLPDWLRNVVPYLPSYPVVQLVRSALLDATLDPKWLTHL
                     LLLAAYGIGATFIAAKLFRWEPKA"
     gene            complement(1170..2099)
                     /locus_tag="VFX11_02430"
     CDS             complement(1170..2099)
                     /locus_tag="VFX11_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4937500.1"
                     /translation="MSQPIIDVQSLTAGYGSTQALKGVNLKVEKGEIFGLLGPNGAGK
                     TTLLAVVEGLHKPKEGSVHIGGINVQADSTATKRQLGIPLQKTALMDDMTVAELVEVY
                     AALYEVYLTSGQINKLLARFDLVEKRNTLARRLSGGQQQRLALAVAIANDPQIVLLDE
                     PTSALDPHARRAVWDIIRQWHAEGRTVVLTTHAMEEAEALCGRIAIIDRGEIVACDSP
                     SALIASLQLNSMLKATVELPLDQVRSLPSVSNARYTGQHLEMETAKPELTLTALHELA
                     ASAGRSVRDVMLRQPNLEDVFLKLTGRALSESN"
     gene            complement(2312..>2833)
                     /locus_tag="VFX11_02435"
     CDS             complement(2312..>2833)
                     /locus_tag="VFX11_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX4937501.1"
                     /translation="CREIARNGLKKIVFVNAHGGNDYLMHYFAQIQLATPRDYVVYVV
                     TPDFSPEDEAMIKAQWETPVDGHAGEKETSAILAIHPNLVHKDKLPTDKEGYPLNRLK
                     SLRDLGVDIGIWWYADYPSHYCGDGIPATVEKGNNWLDARARAFAKVVRAIKADTETK
                     RLQDEFFGRVETF"
BASE COUNT          681 a          782 c          772 g          598 t
ORIGIN      
        1 aacatgccag cgcccacata ccaatgccac ccacaaaata caaaaccagg tttcgcgctg
       61 agacgggcca gccgccacgc gtgatacaga aaaaatataa cagcccttgc gcaatcgaca
      121 acccagccac cgcccactcc cgccaggtca gttcgtttgc acgtgcaatg agaaccccga
      181 cagcagcaag gctgaatatc aataaagagg ctacacccca aaacctgcca tacagctcaa
      241 agtatggggc agggattggg agttcgctat cagccaggtg agatgggcgg gtttctgaca
      301 tgtggaaagt gtactcggga agaggaaaag tggaaagtaa ttatggctca acgtcccgca
      361 ggtttagatc cttcacttca ttcaggacca gcctgcggga cgttattcac ttaggccttc
      421 ggctcccagc ggaacaactt tgcggcgata aaggtcgcgc cgatgccata ggcggcgagc
      481 aggagcaagt gcgtcagcca tttgggatcg agcgtggcat ccagcagagc ggaacgcacc
      541 agttgaacca ccggatagga cggcagataa ggcactacat ttcgcagcca gtccggcagc
      601 atttccaggg gaaagaccag gtcactgatg aacatgagcg aagaatagat ggtcatccca
      661 accgctgtcg ctgtgccggc ttttgccgca actccactga taatgccgcc cagagccatg
      721 aacgccagca ggccagccag gatcatcgga taagccagcg cgaggctcaa ggcactaaca
      781 ggaactttat agagcaaaac accggcgatg atgatcagcg tgctttgcaa aatgccaatt
      841 aggaggttga ccaggagata cgagctgatc aattgtccgg caggcagcgg cgaggcttgc
      901 agccggcgca ggacactctt ttcacgcatc tcaagcatca tgttggaact gccgaccagc
      961 ccgaaactca aagcattcaa cacgataacg ccgggggtca tataatccat gtactgactc
     1021 atgacgactc cgtagatgag catcaacaga actgggaagg caaagttcca gaataacaca
     1081 aagccgtttc gcaagtgcat gaggaatatg gctttggtga ggatcaaaaa tcttttcatg
     1141 ggggttttcc tttcaaaggt aaatatcaat taattggatt cagacagcgc gcgcccggtc
     1201 aatttcagga agacatcttc caggttgggc tgacgcagca tgacgtcacg gacagagcgc
     1261 ccggcagagg cggctaattc gtgaagggcg gtcaaagtca gttcgggttt ggcagtttcc
     1321 atttccaggt gctgaccggt gtaacgcgca ttgctcacgc tgggcaacga ccgcacttga
     1381 tccaggggca attcaacggt tgctttcagc atcgaattca attgcagact ggcaatcagg
     1441 gcgctcgggc tgtcgcaggc tacgatctcg ccgcgatcaa tgatggcgat gcggccgcag
     1501 agtgcctcgg cttcctccat cgcgtgtgtg gtcaggacga cggtgcggcc ctccgcatgc
     1561 cactgacgga tgatgtccca gactgcgcgg cgggcatgcg ggtcaagcgc ggaggtgggt
     1621 tcatcgagca gaacaatttg cgggtcgttg gcaattgcca cagccagtgc caggcgttgt
     1681 tgctgtccac cggagaggcg gcgcgccagg gtattccgtt tttcgaccag gtcaaatcgc
     1741 gccaggagtt tgttgatctg cccagaggtg agatacacct catagagcgc ggcatagact
     1801 tcaaccagct cggccacggt catatcgtcc attaaggcag tcttttgcag tgggataccc
     1861 aactggcgtt tcgtcgcggt tgagtcagcc tggacgttaa ttccgccaat gtgaacgctg
     1921 ccctctttgg gcttatgcaa gccttccaca acggcgagca gggtggtctt gcccgcgccg
     1981 ttggggccca gcaagccaaa gatttcgcct ttttccactt tcaggttcac acctttaagc
     2041 gcctgagtgg aaccatagcc ggctgtcaaa ctttgtacat caataatcgg ttgagacatc
     2101 gttattactc ctttgtcccg ctcgtttcga tatactcttt caagtacgag cagggcgttg
     2161 ttgatgtcaa aatcatacaa aaaaaccggg tgtgaaccca gtcccggaag caggattacg
     2221 actatgtcga aagtcatggt gctggatatg aaaaacgaga ccggctcagg gccggtctcg
     2281 cgtaggctag gacaaaaaac tggttacctc ctcaaaacgt ttccaccctc ccgaagaact
     2341 catcttgtaa acgcttcgtc tccgtatccg ccttgatggc gcggaccacc ttggcaaatg
     2401 cccgggcgcg cgcgtccagc cagttgttac ccttctcgac cgtcgccggg atgccatcgc
     2461 cgcaatagtg actcgggtaa tccgcgtacc accagatgcc tatatccacg cccaaatcac
     2521 gaagagactt cagccgattg agcggatacc cctctttgtc ggtggggagt ttatccttgt
     2581 gcaccagatt tgggtgaatg gccaggatcg cactggtctc tttttcgccc gcgtgaccgt
     2641 ccaccggggt ttcccattgc gccttgatca tggcctcatc ttccgggctg aaatccggcg
     2701 tgaccacata caccacgtaa tcgcgcggcg tcgctaactg gatctgcgca aagtaatgca
     2761 tcaaatagtc gttgccgcca tgcgcattga cgaagacgat cttcttgagt ccgttgcgcg
     2821 cgatctcgcg gca
//
LOCUS       DASUEG010000133         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1745851, whole genome shotgun sequence.
ACCESSION   DASUEG010000133 DASUEG010000000
VERSION     DASUEG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745851"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFX11_02440"
     CDS             complement(<1..1268)
                     /locus_tag="VFX11_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011220302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX4937502.1"
                     /translation="MLDQEQCRQARLSRDRRFDGLFFVGVKTTGIYCRPVCPVRPPLE
                     KNVLYFPSAIAAANAGFRPCLRCRPDSAPGSAAWQGTQTTLQRALRLINAGALQETDL
                     PALADRLGVSDRYLRQLFERELGVSPKQYALYQQSLFAKKLLHETRLPITDIALASGF
                     HSLRRFNDCFRKQFQLAPRAVRKANGAAGGGIQLFLASRPPYHWPLLQRFLAARLIPG
                     LEWCDEHRYGRSFQVGNARGQFTAQFDAGRCGFTIDLALDDLQHLSQVVQNIRRVLDL
                     DADIALIETTIAALFDVPSLPHSGLRLPGIWDPFEAGVRAILGQQVSVAAARNLVQQV
                     VDAFNPDADGIRYFPSAAQLAASNLDFLRMPASRKATLRAFAERATQQAVGDDPAALL
                     GIKGVGPWTVDYIRMRGLSDPDIFLARDLGV"
     gene            complement(1424..1855)
                     /locus_tag="VFX11_02445"
     CDS             complement(1424..1855)
                     /locus_tag="VFX11_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937503.1"
                     /translation="MWFKMKKTVIPVLTLLVAAGLFGARQAYALNDDETAVLVGVAVG
                     GLAAISTKHHLDHDRWDRQRGGYREYHYYEREAPRRYCPPKRVEHYVIHEPPRYREYE
                     REYKRVGYGHGHDRGRHRGWDKHGRRGGGYEYEYEYSRTGF"
     gene            2022..>2229
                     /locus_tag="VFX11_02450"
     CDS             2022..>2229
                     /locus_tag="VFX11_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937504.1"
                     /translation="MKKNLLILLFLLPALMASRCASAGETSQQKFTGKIIANPERVES
                     PADATGATEMKAQAETSASAFSIQA"
BASE COUNT          464 a          698 c          669 g          398 t
ORIGIN      
        1 acgcccagat cccgcgccag aaatatatcg gggtcactga ggccacgcat tctgatgtaa
       61 tcgacagtcc aggggccaac gcccttgata cccagcagcg cggcgggatc gtcgccaaca
      121 gcctgttgcg tcgcccgttc cgcaaacgcg cgcaacgtcg ccttgcgcga cgccggcatg
      181 cgcagaaaat ccaggttgct cgctgccagt tgcgccgctg aaggaaaata ccggatgccg
      241 tcggcgtcgg ggttgaacgc gtccaccacc tgctgcacca gattacgcgc tgcggccacc
      301 gagacctgct ggcccaggat cgcccgcaca cccgcttcga acgggtccca gatgccgggc
      361 aggcgcagtc cgctgtgggg cagcgatggt acgtcgaaca acgcggcgat ggtggtttcg
      421 atcagcgcga tgtccgcatc cagatccagc acgcggcgga tattctgcac cacttgcgac
      481 aggtgttgca gatcgtccag tgccagatcg atcgtgaagc cacaacgccc ggcgtcgaac
      541 tgcgccgtga actggccgcg cgcattgccc acctgaaaac tgcggccgta acggtgttcg
      601 tcacaccact ccagtcccgg aatcagccgc gccgccagaa accgctgcag caagggccag
      661 tggtacggag gacggctggc cagaaacaac tggataccgc caccggctgc accattggcc
      721 ttgcgtaccg cccgtggcgc gagctggaac tgcttgcgga aacaatcgtt gaaacggcgc
      781 aggctgtgaa agccgctggc cagtgcaata tccgtaatgg gcagacgggt ttcatgcagc
      841 agcttttttg cgaacaggct ttgctggtac agcgcatatt gcttgggcga gacgcccagc
      901 tcgcgctcga acagctggcg caggtaacga tcgctgacgc cgagccggtc cgccagcgct
      961 ggcagatccg tttcctgcag ggcaccggca ttgatcaacc gcaatgcacg ctgcagggtg
     1021 gtctgggtgc cttgccaggc cgccgacccc ggcgcactgt cgggccggca gcgcagacag
     1081 ggccggaaac cggcattggc tgcggcaatg gcgctgggga aatagagcac gttcttttcc
     1141 agcggcggac gcaccgggca gacgggacga caataaatgc ccgtggtttt cacgcccacg
     1201 aaaaacaggc cgtcgaaacg acgatcgcga ctgaggcggg cctgacggca ttgttcctgg
     1261 tctagcatgg gcgttttccg gtggcgtgtt tcccgcattg taacccggcg ccccggcaaa
     1321 accggccgga tccggaactg gtcccaccct gattgcgcct gcccaaaaaa cagactgcgg
     1381 cccgaaggcc gcagtctggt gttgccggtt acaaggtcag ggatcagaag ccggtgcggc
     1441 tgtactcgta ttcatactcg tagccgccac cacgacgtcc gtgcttgtcc caaccgcggt
     1501 gacgaccacg gtcgtgaccg tgaccataac ctacccgttt gtattcacgc tcgtactcgc
     1561 ggtaacgggg cggttcatgg atcacgtagt gctcgacccg tttcggcggg cagtaacggc
     1621 gcggggcttc ccgctcataa tagtgatatt cgcggtagcc accccgttgc cggtcccaac
     1681 gatcatgatc caggtggtgc ttggtggaaa ttgccgccag accaccgacc gccacaccga
     1741 ccagcacggc ggtttcgtcg tcattcagtg cataggcctg acgggcgccg aacagaccgg
     1801 ctgcaaccaa cagggtcaat accggaataa cggttttctt catcttgaac cacataagga
     1861 attcctcctt gttgtttgac tgttcctgca gattaaaccc ggtcggctga attgatcctg
     1921 aacgcctgca tccgtttgat tcagtttcga ttcagtggcc ggccgctgta atggcgccac
     1981 aacaagcggc aatggcgtcg cgacggaacg gaggcacacc catgaaaaaa aacttgctca
     2041 tactgttatt tttactgcca gcactgatgg ccagtcgctg cgccagtgcc ggggaaacca
     2101 gccagcagaa attcaccggc aagatcatcg ccaatccgga acgggtggaa tcacctgcgg
     2161 atgcgacagg cgcgacagag atgaaagcgc aagcggaaac cagcgccagt gcgttcagca
     2221 tccaggccc
//
LOCUS       DASUEG010000134        10834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1818870, whole genome shotgun sequence.
ACCESSION   DASUEG010000134 DASUEG010000000
VERSION     DASUEG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818870"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFX11_02455"
     CDS             complement(<1..861)
                     /locus_tag="VFX11_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX4937505.1"
                     /translation="MNRALQAALQHRLAEALGSAYASAQFEPSGTGCINETWAARAPG
                     VEPVFIKLGSAEALAMYEQEALGLDTLRRCESIRVPGVHFYDRIESDDGAVAAVLVLA
                     FIALTSPRAQDDAPVGEALAFLHDIQGPAFGFQADNFIGRTPQINAWREDWWDFWCQN
                     RLQPQLRLAQLRGMRTSLLDRLQRLIDLVPARFAAHRPQPSLLHGDLWSGNLAVDEQG
                     RPVLFDPAVYFGDAETDLAMLKMFGGVRPGVFAAYHQRRPESPQSQQRRNLYDLYHWL
                     NHFNLFGVGYL"
     gene            complement(858..1757)
                     /locus_tag="VFX11_02460"
     CDS             complement(858..1757)
                     /locus_tag="VFX11_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX4937506.1"
                     /translation="MANPDTVYLLDASIYVFRAWFGLPDSIRDQRGRPVNAVLGYWRL
                     LLEQLSRLKPRLGLAAFDESLFSGFRHLLYQPYKSHRALPDEELAYQLELCRLLTEAL
                     GIPCRSSQIYEADDVLATASRLARGNGLPVTLLTRDKDLAQLVAPGDHWWDWSGGAPL
                     DHAALCQRWGVPPAQIPDLLALAGDAVDSIPGVLGVGIQSARALIRHFGNLENLYRRL
                     DELPALPLRGSARIQRALQAQQHEAFLFRELIRLHDADDLDEPISLAGLELDSPDPLY
                     LRALIGELQLGRAFLTPLERYFS"
     gene            complement(1810..2016)
                     /locus_tag="VFX11_02465"
     CDS             complement(1810..2016)
                     /locus_tag="VFX11_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937507.1"
                     /translation="MAFVVGEAYREPDDRKWQANARGNILELSPLLESAQSRRLDRLL
                     KMCAGYPNPPVSGPQGARFWQTAF"
     gene            2044..4992
                     /locus_tag="VFX11_02470"
     CDS             2044..4992
                     /locus_tag="VFX11_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4937508.1"
                     /translation="MSNNILQLRKALAIGTLSALSISLSACLSPQGGGKPATLADLQR
                     RDVVIQQQQLDPVEAERVIDTYQQAIDLFSSSEERSLALRRMADLTMVATEDRLIESA
                     DETPEVMAAGDAAAAAKVKQASGGSLQYEKAIALYESLIASAPKGADLAEEYYLLSKA
                     YDLNGQTDKALATLDTLVTRYPNSDFTAEAQFRRGEQFFLLGRYQDAADAYGYVLKAG
                     RDNEYYEHSLYKHGWSLYKLGDFDLAVNDFVALLDTYMPTPPPKSPEQIKAENKDRAL
                     PKIEAIPLPQVSGTQRKIMDDTLRVLSMSFSNLEGADSIYQYFRQNGSRIYEHEVYQA
                     LGELYLLQERYKDAADTFAMFAKKNPLHPMAPSMSSREIATYQKGGFPSLVLPAKENF
                     VKQYGVYSAYWERATPEAREQYSTDLKLHLVELANHYHAQAQTTKLPDDYMKAARWYR
                     EFLATWPNDQTAPTMNMLLAEVLFAAKDFDGAIEEFERTAYDYPAHPGAEKAGYFALL
                     AHQEHIKLTPKDDPIYRGLIAKRASSSLRFAKAYPTNAHTPEILDGVIEDELTLGDMD
                     GVIVATQLVIGLVPPAPQPLREKAWTTYANAVYDKQQYREAETAYSKVLEFKSLSAKD
                     RANYQERLATSVYKQGEALEKAGKLSEAAGEYLRVGVVAPAATVRANAEYDAANILLQ
                     QQQYDKAIPVLEKFRKTFPNHELAKSVPAKLSMAYEQTGNLGAASTELVAIAALNKGS
                     DPELARQALWQAAGMKEKSGDNSAALKLYEQYEREFPNPVGPRMEAQYKAATFNEKAG
                     NSSQRLVYLNKLVESHMRAGSEANDRTRYLAAYGSYNAADVLFQNFSAIRLTQPLKAS
                     IARKKDAMQKASDAYTNTVKFGVIDFTTGANYKLGELYRQFAESIMQSERPRGMDDAA
                     LEEYEILLEDQALPFEDKAIAIFQTNAERTKDGVWDEWIEKTYESLRKLSPGRYSKTE
                     LVEDSVDVIY"
     gene            4979..5770
                     /locus_tag="VFX11_02475"
     CDS             4979..5770
                     /locus_tag="VFX11_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4937509.1"
                     /translation="MSFINGSATFRRHTLSALALALLLSACSSNEPKEEEFAMPESVV
                     VDLEGGDAKAAAPAAEAAATEGDAAAADAKKATTDQDSAAVLERLRTQAAPVTARDPA
                     HEKQAKAAKDGFDRALVEMKKGNLDGALAQFRQLSAQYPALAGPVVNQAIILRKKGQL
                     DAAHKLLQDGLMTHGRNPYYLNQLGIYSREQGQFRKAQASYESAIRIDENYAKAHYNL
                     AVLADLYLHDPALALREFQAYQKLLPEPDAKVAGWIKDLERRQAP"
     gene            5789..6073
                     /locus_tag="VFX11_02480"
     CDS             5789..6073
                     /locus_tag="VFX11_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937510.1"
                     /translation="MKRILYWAALTLLPLHALALDEGTTIIGTKEAPNVLNVVPWQSR
                     ELGVDPWGAQPTHNSELLNDALKPVDRDELQRQVEYFNLLQDSSPQAVEP"
     gene            6131..6838
                     /locus_tag="VFX11_02485"
     CDS             6131..6838
                     /locus_tag="VFX11_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4937511.1"
                     /translation="MESTTAAINAAETSAGFFPTLVQFFVEGGVFMYVILFVFLLGLA
                     IAVERFIYLNLVQTRNQTVWKKLFPLLTQGKFKSAMDEAKTSTAAVAKILVYGLSRSA
                     YATHHDEVEMAMEEGLMEAIPSLERRTHYIAIFANIATLLGLLGTIQGLITAFDAVAG
                     ADPAQKGDLLSASISVAMNTTAFGLIAAIPLMLIFAYLQSKTNQLIDSMEMASVKFLN
                     VFRQAQNQSKNKPSGTP"
     gene            6875..7423
                     /locus_tag="VFX11_02490"
     CDS             6875..7423
                     /locus_tag="VFX11_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4937512.1"
                     /translation="MRRRTLRSRDTEVQLNITTFMNLMVVLVPFLLITAAFSEMSVLQ
                     LTLPPTSAVPQQQQDEQKKPIVLEVLIYKNRLEVVDRQTGPLKIIPVTSTGHDYKTLT
                     TLLKAVKLRFPEITDVTLLLERDTAYDVLVQTMDHVRMTYQLQGGTSVEVELFPDIAI
                     GDAPPDVQAVPQAAPAVPGGDA"
     gene            7420..7926
                     /locus_tag="VFX11_02495"
     CDS             7420..7926
                     /locus_tag="VFX11_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4937513.1"
                     /translation="MKLSARAKRMERRRKRSKFPEINLVSMMDIFTILVFFLLVNSSD
                     AIQPPNQKVVKLPQSTAEMLPDQTVSILVNDQAILVNGHAVIDVPSVMAMPSDMIPAL
                     QKELRYQASKSVAPVDELGVAQRDITIMGDKAIPYGLLRKIMVTCSNSEYTNISLAVL
                     KKPADKNS"
     gene            7923..8918
                     /locus_tag="VFX11_02500"
     CDS             7923..8918
                     /locus_tag="VFX11_02500"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX4937514.1"
                     /translation="MIETPYFSTPTLPWDRRAEEEKLFRRLAALFILIFFIITLIVPY
                     IPLPEKQREEVERIPPRLAKLVMEKQKPPPPKVEEKPKEELKEEPKKEEPKKEEPKKE
                     EKKEEPKPKKEEPKKPQPTKQEAAKKQAAAAVAVFDDLADLRDTQDLANLKVEQQSVT
                     SASVSAQGTATDRALIGRAAIGGSGGVAVAKASSGGGGTGKLGTAPGAGMQSSQVKSA
                     IADPAAQQAEKRGKGGKARRTTEDVQLVFDRNKGAIYSLYRRALRENPALEGTLVLRL
                     EIQPNGAVSRCEVVSSELKDPDLERKIVTKVKSINFGKMDVDVWNDSYPISFIPS"
     gene            8931..9434
                     /locus_tag="VFX11_02505"
     CDS             8931..9434
                     /locus_tag="VFX11_02505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX4937515.1"
                     /translation="MIKSLASVLMLIATLVVTGPALAKKPPVGDQAPDFTLHADGPFN
                     LRLEEQKGYVVVVGFWASWCRSCPIQLKALETLHQKYKDMGVKVWAITLDEDFEAARH
                     HAHKHGLNFTLLQDGEVRVSERYDIDDLPATFVVDRDGKLRLMTEGFNPGDEARLDQF
                     LETLVRE"
     gene            9475..10284
                     /locus_tag="VFX11_02510"
     CDS             9475..10284
                     /locus_tag="VFX11_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937516.1"
                     /translation="MTPVTRWSTSFSRSLLLGFSLLTLLLSLSAPLQAAKPADDIDAD
                     IDDAELEYVEIDEILSEDENAPRPSKADQAAKKKKAQVEAASTDEEKEFVDLNQILYG
                     GEQPAKGGKGAAANAPAPDRKSMQQEMQSLKEKVLQVNRDLFVLEEDLLYPSSTQVNV
                     FVSCDALPYFQLDGVTVKINDRAVGNHLYTERELKAMNRGAVQRLFTGNLPVGEHELV
                     AIVSGVGPEGRDYKRAVSIEFEKTSGTKYIELKIEGDNQRQQPTFRLKQWE"
     gene            10304..>10834
                     /locus_tag="VFX11_02515"
     CDS             10304..>10834
                     /locus_tag="VFX11_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937517.1"
                     /translation="MKSIFSLIKPAPAPQPLRALTLALLLAGTPALAAEPEAATETPP
                     AAQAPATTSIQSADEGGFMAPVKKAFGSVSDSVTHLFSFGEDDAAEKPAAETAPAQPI
                     PIDQPHPVGDLAYGDLLFEYYQQRYFAAITKILVAQQRGLLSRDAEHTQILLGALYIS
                     YGMLDKGEAIFRQLLAG"
BASE COUNT         2526 a         3365 c         3094 g         1849 t
ORIGIN      
        1 caggtaaccc acgccgaata ggttgaaatg attgagccag tggtacagat catacaggtt
       61 gcggcgctgc tgcgattgcg gtgattcagg ccgccgctgg tgataggcgg caaatacgcc
      121 gggccgcacg ccgccaaaca tcttcagcat ggccagatcg gtttcggcgt caccaaaata
      181 aacggcggga tcgaacagta ccgggcggcc ctgctcatcc accgcaagat tgccgctcca
      241 cagatcgccg tgcaacagcg atggctgggg ccggtgtgct gcaaatcgcg cgggaaccag
      301 gtcaatcagc cgctgcagcc ggtccagcag actggtgcgc atgccacgca gctgcgccag
      361 acgcaactgg ggctgcaggc ggttttggca ccagaaatcc caccagtctt cacgccatgc
      421 attgatctgg ggcgtacggc cgatgaaatt gtcagcctga aagccgaagg ccgggccctg
      481 gatgtcatgc aggaatgcca gcgcctcgcc aacgggggca tcgtcctgcg cacgcgggga
      541 tgtcagcgca ataaaggcca ggaccagcac ggctgcaacc gcgccgtcat cgctttcaat
      601 ccgatcgtaa aaatgcacgc cgggcacccg gatcgactca caacgccgca acgtatccag
      661 gcccagcgct tcctgctcat acatggccag cgcctctgcg ctacccagct tgatgaaaac
      721 cggctccacg cccggcgccc gcgccgccca ggtttcattg atgcagccgg tgccggacgg
      781 ttcgaactgc gcgctggcat aggcagaacc cagcgcctcc gccagccggt gttgcaacgc
      841 tgcctgcagg gcccggttca tgaaaaatag cgctccagtg gagtgagaaa cgcgcgtccc
      901 agttgcagtt caccgatcag cgcccgcagg taaagggggt cgggagaatc cagttccagg
      961 ccggcaaggc tgatcggctc gtcgagatca tccgcgtcat gcagccggat cagttcccga
     1021 aacagaaagg cctcgtgctg ctgtgcctgc aaggcccgtt gaatccgggc actgccacgc
     1081 aggggcaagg ccggcagttc atccaggcga cggtagagat tttccagatt gccaaaatgc
     1141 cggatcagcg ccctggcgga ctggatgcca acgcccagca caccaggaat gctgtccacc
     1201 gcatcacccg ccagggccag cagatcgggt atctgcgccg gcggcacacc ccagcgttgg
     1261 cacaatgccg catgatccag cggcgcaccg ccggaccagt cccaccagtg atcacccggc
     1321 gccaccagct gtgcaagatc cttgtcccgg gtcagcaggg tcaccggcag gccgtttcca
     1381 cgggccaggc ggctggccgt ggccagaaca tcatcggcct catagatctg gctggaacgg
     1441 cacggtatgc ccagggcttc cgtcagcaaa cggcacaatt cgagttgata ggccagctct
     1501 tcatccggca gcgcccgatg ggatttatag ggttgataga gcaggtgacg gaagccactg
     1561 aacaaacttt catcaaacgc agccagtccc aaacggggct tcagtcgcga cagctgctca
     1621 agcaaaagac gccagtaacc cagcaccgca ttcaccggcc gtccccgctg atcccggatc
     1681 gaatcgggca gtccaaacca cgcgcggaac acatagatgc tggcatccag cagataaacc
     1741 gtgtcgggat tcgccaattt cgcgtttttc caatataacc atttgataat tgagaaaatg
     1801 aactatacct tagaaggccg tctgccaaaa gcgcgcgcct tgcgggccgg aaacaggcgg
     1861 atttggataa ccggcgcaca ttttcagcaa gcgatccagg cgccggcttt gcgcactttc
     1921 caaaagcggc gacaattcaa gaatattgcc gcgtgcgttt gcttgccatt tgcgatcatc
     1981 gggttccctg tacgcttcac caacgacaaa cgccataaaa agaagtcacg ggttttcaga
     2041 cgagtgagta acaacatcct acaactgcga aaagcgctgg caatcggcac cctctctgcc
     2101 ctttccatca gcctgagcgc ctgtctctca ccccagggtg gcggcaaacc ggcaacgctg
     2161 gcggacctgc agcggcggga tgtggtgatc cagcagcagc aactggatcc ggtggaagcg
     2221 gaacgtgtca tcgacacgta tcagcaagcc atcgatttgt tctcctcttc cgaagaacgc
     2281 agcctggcct tgcgccgcat ggccgacctc accatggtcg ccaccgaaga ccgcctgatc
     2341 gaatccgctg acgaaacccc tgaagtcatg gccgccggcg acgccgcagc agccgcaaag
     2401 gtcaagcagg ccagtggtgg atcgctgcag tatgaaaaag ccattgccct gtatgaatcc
     2461 ctgatcgcct ctgccccgaa aggcgctgac ctggcggaag aatattatct gctgtccaag
     2521 gcgtacgatc tgaacggtca gaccgacaag gcccttgcca cgctcgacac cctggtgacc
     2581 cgttacccca acagtgattt caccgccgaa gcgcaattcc gccggggtga acagttcttc
     2641 ctgctggggc gctaccagga cgccgccgat gcctacggtt atgtactcaa agccggtcgc
     2701 gacaacgaat actacgagca ttccctctac aagcacggct ggagcctgta caagctgggg
     2761 gatttcgatc tggcggtaaa cgatttcgtg gcgctgctgg acacctacat gcccaccccg
     2821 ccgccgaagt cgccggaaca gatcaaggcg gaaaacaagg atcgcgcgct gcccaagatc
     2881 gaggccattc cgctgccgca agtcagcggc acccagcgca agatcatgga tgacactctg
     2941 cgtgtgctga gcatgtcgtt ctcgaacctg gaaggcgcgg acagcatcta ccagtatttc
     3001 cgccagaacg gctcgcgcat ttacgagcat gaggtgtacc aggccctggg cgaactgtac
     3061 ctgctgcagg aacgctacaa ggacgccgcc gacacctttg cgatgttcgc caagaaaaac
     3121 ccgctgcacc cgatggcgcc gtccatgtcg tcgcgggaaa tcgccaccta ccagaaaggt
     3181 ggcttcccca gcctggtgtt gccggcaaaa gagaatttcg tcaaacagta tggcgtgtac
     3241 agtgcctact gggagcgcgc cacccccgaa gcccgcgagc aatacagcac cgacctgaaa
     3301 ttgcacctgg tggaactggc caatcactac cacgcccagg cccagaccac caaactgccc
     3361 gacgattaca tgaaagccgc gcgctggtat cgtgaattcc tggccacctg gccgaacgac
     3421 caaaccgcgc ccaccatgaa catgctgctg gcggaagtgc tgttcgccgc caaggatttc
     3481 gacggcgcga tcgaggaatt cgaacgcacc gcctacgatt accccgcaca tcccggtgcg
     3541 gaaaaagccg gctattttgc cctgcttgcg caccaggaac atatcaagct gacaccgaag
     3601 gacgatccca tctaccgtgg cctgattgcc aaacgtgcca gcagttcgct gcgtttcgcc
     3661 aaggcctatc ccaccaacgc ccacaccccg gaaattcttg acggcgtgat cgaggacgaa
     3721 ctcacattgg gggatatgga tggcgtgatc gttgccaccc agctggtgat cggtctggta
     3781 ccgccggcgc cgcagccgct gcgtgaaaaa gcctggacca cctacgccaa cgctgtttac
     3841 gacaagcagc agtaccgcga agcggaaacc gcctattcca aggtcctgga attcaagtcc
     3901 ctgtctgcca aggatcgcgc caactaccag gaacgcctgg ccaccagcgt gtacaaacag
     3961 ggcgaggcac tggaaaaggc cggcaagctg tcggaagcgg caggcgaata cctgcgggtg
     4021 ggtgtagtgg cgcccgccgc caccgtgcgc gccaacgccg aatacgacgc cgccaacatc
     4081 ctgctgcaac agcaacagta cgacaaggca attccggtgc tggagaaatt ccgcaaaacc
     4141 ttccccaatc atgaactggc caagtccgtt cccgccaagc tgagcatggc gtatgaacag
     4201 accggcaacc tgggcgccgc ttccaccgaa ctggtggcca tcgccgcact gaacaagggc
     4261 agcgatccgg aactggcacg gcaggccctg tggcaagcgg caggcatgaa ggaaaaatcc
     4321 ggcgacaaca gtgccgccct gaagctgtat gaacaatacg agcgggaatt ccccaacccg
     4381 gtgggcccgc gcatggaagc ccagtacaag gccgccacct tcaacgaaaa agccggcaac
     4441 agcagccagc gcctggtgta cctgaacaag ctggtggaat cccacatgcg cgccggcagc
     4501 gaagccaacg accgcacccg ctacctggcc gcctacggct cctacaacgc cgccgacgtg
     4561 ctgttccaga atttcagcgc catccgcctg acccagccgc tgaaagccag catcgcgcgc
     4621 aagaaagacg ccatgcagaa agccagcgac gcctacacca ataccgtgaa attcggcgtc
     4681 atcgatttca ccaccggcgc caactacaag ctgggtgaac tctaccgcca gttcgccgaa
     4741 tccatcatgc agagtgaacg cccccgcggc atggacgacg cggcgctgga ggaatacgaa
     4801 atcctgctgg aagaccaggc tctgcccttc gaggacaaag ccatcgccat cttccagacc
     4861 aacgccgaac gcaccaagga tggcgtctgg gatgagtgga tcgagaaaac ctacgaatcc
     4921 ctgcgcaagc tgagcccggg ccgttacagc aagaccgaac tggtggagga ctccgtcgat
     4981 gtcatttatt aacggttccg ccacattcag acgccacacc ctcagcgccc tcgccctcgc
     5041 tctgctgctg tctgcctgtt ccagcaacga gccgaaggaa gaggaattcg ccatgccgga
     5101 atcggtggtg gtggatctgg agggtgggga tgccaaggct gccgcccctg cagcagaggc
     5161 agcagccact gaaggcgatg cggccgcagc cgacgcaaaa aaagccacga ctgatcagga
     5221 ttctgctgcc gtgctggagc gcctgcgcac tcaggcagcg ccggttaccg cccgcgatcc
     5281 cgcgcacgaa aaacaggcca aggcagccaa agacggcttc gaccgtgccc tggtggaaat
     5341 gaaaaaaggc aacctggatg gcgcgctggc gcaattccga cagctgtcgg cacaataccc
     5401 cgcgctggcc ggtccggtgg tgaaccaggc gatcatcctg cgcaagaaag gccagctcga
     5461 cgcggcgcac aaactgctgc aggacggtct gatgacgcac ggccgcaacc cttattacct
     5521 gaatcagctt ggcatctaca gccgcgaaca gggccagttc aggaaagcgc aggccagcta
     5581 tgaatctgcg atcaggatcg acgaaaatta cgccaaggca cactataatc tggcagtgct
     5641 ggcggatctt tatctgcacg atccggcgct ggcgctgcgg gaatttcagg cttaccagaa
     5701 actgttgccg gaaccggatg caaaagtggc tggctggatc aaggaccttg agcgccggca
     5761 ggcgccctga cgtgaagcga gaacgaacgt gaaacggata ctgtactggg ctgctctgac
     5821 tctgctgcca ctgcacgcac tggcactgga cgaaggcacc accattatcg gcaccaagga
     5881 agcgcccaac gtgcttaacg tggtgccctg gcaatcccgc gaactgggtg tcgatccctg
     5941 gggcgcgcag ccaacgcaca attccgaatt gctcaatgac gcattgaaac cggtggaccg
     6001 ggacgagttg cagcggcagg tggaatattt taacctgctg caggattcat caccgcaagc
     6061 cgttgaacca taagaatttt ttaaatcgaa tcccaccaaa taaagatatc gggtgaatca
     6121 ggagactcta atggaaagta caaccgccgc catcaacgcc gcagaaacca gtgcgggatt
     6181 tttccccact ctggtgcaat tctttgtcga gggtggcgtg ttcatgtatg tcatcctgtt
     6241 tgtttttctt ctcggcctgg cgatagccgt cgagcgtttc atctatctga acctggtgca
     6301 gacccgaaac cagaccgtgt ggaaaaagct gttcccactg ctgacccaag gcaaattcaa
     6361 aagcgcaatg gatgaagcga agacatccac tgccgcggtt gcgaaaattc tggtgtatgg
     6421 actatcccgt agtgcctatg ccacccacca cgacgaagtg gaaatggcca tggaggaggg
     6481 gctgatggaa gccatcccca gtctggagcg ccgcacccat tacatcgcca tcttcgccaa
     6541 cattgccacc ctgctcgggt tgctcggcac catccagggc ctgatcaccg cattcgatgc
     6601 cgtggccggc gcagatcccg cccagaaagg cgacctgctc tccgccagta tctcggtggc
     6661 gatgaacacc actgcattcg gtctgatcgc cgccattccg ctgatgctga tcttcgccta
     6721 cctgcagtcc aagaccaacc agctgatcga cagtatggaa atggcatcgg tgaaattcct
     6781 caacgtattc cgtcaggccc agaaccagag caagaacaaa cccagcggta ctccctgatt
     6841 gcgctcgcca gactgaacag gagttttcat ctgtatgcgt agacgcacgc ttcgcagccg
     6901 ggacaccgag gtccagctca acatcaccac cttcatgaat ctgatggtgg tgctggtgcc
     6961 gttcctgctg attacggcgg ccttctcgga aatgtcggtc ctgcagctga cgctgccacc
     7021 cacttcagct gttccgcagc aacagcagga cgagcagaaa aaacccattg tcctggaagt
     7081 actgatctac aagaaccgac tggaagtggt ggaccgccag accggcccgc tcaaaatcat
     7141 tcccgtgacc agcaccggtc acgactacaa gacgctgacc accctactga aggcagtcaa
     7201 actgcgcttt cccgagatta ccgatgtgac tctgctgctg gaacgggaca cagcttacga
     7261 tgtcctggtg cagaccatgg atcacgtgcg catgacctat cagctgcaag gtggcacttc
     7321 cgtcgaggtg gagctgttcc cggacatcgc tatcggtgat gcaccaccag atgtccaagc
     7381 cgtcccgcaa gctgcgcccg ccgtgccagg aggcgatgca tgaagctgtc cgcccgcgcc
     7441 aagcgcatgg agcggcgccg caagcgctcc aagtttccgg aaatcaactt ggtgtcgatg
     7501 atggatatct tcaccattct tgtgtttttc ctgctggtga attcgtctga cgcgatccag
     7561 ccacccaacc agaaagtggt gaagctgccg cagtccaccg ccgagatgct gccagaccag
     7621 accgtttcta tcctggtgaa cgatcaagcc atccttgtca atggccatgc tgttatcgat
     7681 gtgccgtccg tcatggccat gcccagcgat atgattcccg ccttgcagaa ggaactgcgc
     7741 tatcaggcca gcaaatccgt tgcaccggtg gatgaactgg gtgtcgccca gcgcgatatc
     7801 accatcatgg gcgacaaggc aattccctac ggcctgctgc gcaagatcat ggtgacctgc
     7861 agcaatagcg aatacaccaa catttcgctg gcagtactca agaaaccggc ggacaagaac
     7921 tcatgatcga gactccgtat ttttcgacgc cgaccctgcc ctgggacaga cgggcggagg
     7981 aagaaaagct gttccgccgg ctggcggcgc tgttcatcct gattttcttc atcatcaccc
     8041 ttatcgtgcc gtatattccc ctgccggaaa aacagcgcga ggaagtggag cgcattccgc
     8101 cacgcctggc caagctggtg atggaaaagc agaaaccgcc accgccgaag gtggaggaaa
     8161 agccgaagga agaattgaag gaagaaccga aaaaagaaga gccgaaaaag gaagaaccta
     8221 aaaaagaaga gaagaaagaa gaacccaagc ccaagaagga agagcccaaa aagccgcagc
     8281 ccaccaagca ggaagccgcg aaaaaacagg cagcggcagc agtggcagta tttgatgatc
     8341 tggcggatct gagagatacc caggacttgg ccaacttgaa agtggagcag caatcggtga
     8401 ccagcgccag cgtcagcgct caaggcaccg ctactgatcg tgcgctgatc ggcagggccg
     8461 cgattggtgg cagcggagga gtggccgtcg cgaaagccag ctctggcggt ggcgggaccg
     8521 gcaaactggg tactgcccct ggcgccggca tgcagtccag ccaggtgaag agcgccatag
     8581 ccgatcctgc ggcgcagcag gcagaaaaac gcggcaaggg tggcaaggcc cgccgcacca
     8641 ccgaagacgt ccagctggta ttcgaccgta acaagggcgc catctattcc ctgtatcgcc
     8701 gtgccctgcg ggaaaatccg gcgctggaag gcacgctggt gctgcggctt gaaatccagc
     8761 ccaatggcgc cgtctcccgc tgcgaagtgg tgagcagcga gctgaaagac ccggatctgg
     8821 agcgcaagat tgtcaccaaa gtgaagagca tcaattttgg caagatggat gtggatgtgt
     8881 ggaatgactc ctatccgatt tccttcattc cttcctgacg gggcactgtc atgatcaagt
     8941 cccttgcctc cgtcctgatg ctgattgcca ccctggtggt gacaggccct gccctggcca
     9001 agaagccacc ggtgggcgac caggcgccgg atttcacgct gcacgccgac ggtcccttca
     9061 atctgcggct ggaagaacag aaaggctatg tggtggtggt gggattctgg gccagctggt
     9121 gccggtcgtg tccgatccag ctcaaggcgc tggaaacgct gcaccagaaa tacaaggaca
     9181 tgggcgtgaa ggtctgggcc atcacgctgg atgaggattt tgaagccgcg cgccatcacg
     9241 cccacaagca cggcctgaat ttcaccctgc tgcaggatgg cgaagtgcgg gtgagcgagc
     9301 gctacgacat cgacgatctg cccgccacct ttgtggtgga ccgggacggc aagctgcggc
     9361 tgatgacgga aggtttcaat ccgggtgacg aagcccggct ggaccaattt ctggaaaccc
     9421 tggtgcgcga atgaccgcac accctgcaag aaccacgcat agagcatgac gacaatgaca
     9481 cctgtgacac gctggtccac ctcattttcg cgatccctac tgctgggatt ctccctgctg
     9541 acgctgctgc tcagcctgtc ggcgccgctg caggccgcca agccagcgga tgatatcgac
     9601 gcggacattg atgacgccga actggaatac gtcgagatcg acgaaatcct gagcgaggac
     9661 gaaaacgcgc cccgtccgag caaggccgat caggctgcca agaaaaagaa agcccaggtt
     9721 gaagcggcca gtaccgacga ggaaaaggaa ttcgtcgacc tgaaccagat tctgtatggc
     9781 ggcgaacagc cggccaaggg cggcaaggga gccgctgcga acgcgccggc acccgatcgc
     9841 aaaagcatgc agcaggaaat gcagtcgctg aaggaaaagg ttctgcaggt gaaccgcgat
     9901 ctgttcgtgc tggaagagga tctgctctac cccagcagca cccaggtcaa cgtcttcgtg
     9961 tcctgcgatg ccctgcccta cttccagctg gacggcgtga ccgtgaagat caatgaccgc
    10021 gccgtaggca atcacctgta caccgagcgc gaactcaagg ccatgaaccg gggcgcggtg
    10081 cagcgcctgt tcaccggcaa cctgccggtc ggcgagcatg aactggtggc catcgtcagc
    10141 ggcgtgggcc ccgagggccg cgattacaag cgcgcggtca gcatcgaatt cgagaaaacc
    10201 agcggcacca agtacatcga actcaaaatc gaaggggata accagcgtca gcagcccacc
    10261 ttccgtctga aacagtggga gtgagcgggc tgaggacacg gcattgaaat ccatcttctc
    10321 attgatcaag ccggccccgg caccgcagcc cctgcgggcg ctgacgcttg ccctgctgct
    10381 ggcaggcacg ccggccctgg cagccgaacc ggaggccgcg acagaaacgc caccggcagc
    10441 ccaagccccg gccaccacca gcatccagtc tgccgatgaa ggcggattca tggcgccggt
    10501 taaaaaggct tttggttccg tttcggattc cgtcacccat ctgttcagct tcggcgagga
    10561 cgacgctgcg gagaagcctg cggcggaaac cgcccctgcc cagccgatcc ccatcgacca
    10621 gccccatccc gtcggcgacc tggcctatgg cgacctgctg ttcgagtact accagcaacg
    10681 ttatttcgct gccatcacca agattctggt ggcgcagcag cggggcctgc tcagccgcga
    10741 cgccgagcac acgcagatcc tgctgggtgc gctctacatt tcctacggca tgctggacaa
    10801 gggcgaagcc attttccgcc aactgctggc cggt
//
LOCUS       DASUEG010000135         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1377074, whole genome shotgun sequence.
ACCESSION   DASUEG010000135 DASUEG010000000
VERSION     DASUEG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377074"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VFX11_02520"
     CDS             <1..2044
                     /locus_tag="VFX11_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4937518.1"
                     /translation="LQELADDPLQRIFQYTRGWPAGVKLAQVYLQRSNRTLSPGQDLF
                     GKHVFVDLCNGVLQTLPPALQQFLVQTALLESFSAPLCDYMLHSAHGAEHIRQLQELG
                     LFIEADPAQSAFRYHALFRHHLLQLFRQTPLEQQDRVIARACTWLMDSGQREAACRLA
                     REHSQRGFFVEVLQRSFHAWFKAGRGDPVFYWARELGEKALLALPETRFAWCWALTMF
                     GRWDDAESLLVRTPVSAESADQMQPDPASLVLQSIIRLFRCELDDSQIELLQRLYKLP
                     ELARDLRASIDNILAQHAIHRCQYQEAWKRASQAALMLAQNGNLFGQTLAQYLIANAS
                     FQNNDIRNALATCDQYLEQQPLDADHPSRALLAGFRAYLLYQGEQPLQAEQLIHDVLA
                     GSQPGYSIDLQLFLYVPLIRLRARRGQFVAAHELLEELEAAASASASPLFQAHTRFEA
                     LRLAYAKGNGNELQRLAAQYGVEEAARKALDPNLPLQWETREYRIMAGIIVYMHQEQF
                     DRARALALQLLHLNVDHGYPIRFLPINMYIAYLDLCLGQISSAYRRLNDTLTQAEATG
                     MLMGLIDDLPGLDDMLRLALSHQRILNPEHVRRIRAMGILELQAPAQTPALTPPELDI
                     TRLLLQGLNVHDIADHLQLSLTTVQWHLQRILYKLDARQIGDLVPRLRQLDILPSS"
     gene            complement(2039..3046)
                     /locus_tag="VFX11_02525"
     CDS             complement(2039..3046)
                     /locus_tag="VFX11_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4937519.1"
                     /translation="MNVGRVDLNLLVYLDVLLRELNVTRAAEQLGITQPAMSNGLKRL
                     RELFNDPLLIRSSDGMKPTERALDLQPKVRQILAETQYLLEPRQEFKPLTSKRVFRIM
                     VSDYAEATLIPALIKHIRSEAPHIILDMLTPSDVSYQDMEQGRVDMAINRFDEIPQSF
                     HQTVIWKDSFSCLINRDNPIVHNFNLKTYLLAQHIWVSKTGMGVGFGINPEKSGGLGW
                     IDSALARIGKKRKITIFTRHYQMPGLLAQNNDLVATLPTRVARMQAENPCLVITEPPF
                     YIPPFELKMAWSPLLHPNAAHQWLRRLIMYTAERLLEAEPLVRLQEESAVNRPLDADR
                     L"
     gene            complement(3138..>3680)
                     /locus_tag="VFX11_02530"
     CDS             complement(3138..>3680)
                     /locus_tag="VFX11_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937520.1"
                     /translation="TAIGQQGRLLAWLAFEKIRTWPGRFGPASMVRLCAPPQMQGMLE
                     KMGQMSGFHGLGGADFMRDENGNLFALEQHGRPNGQFVLADQVGIDLPALLRTLILGS
                     EHSGEVITSLQRVQPPQIRDTHWLPLFPQEAERQLAQGQWRTLRRWLLHPRYRAMLPL
                     DDPALLDAELRRLQQRAQTR"
BASE COUNT          680 a         1134 c         1140 g          726 t
ORIGIN      
        1 gcttcaggag ctggccgacg atccgctgca acgcattttc cagtacaccc gtggctggcc
       61 agcgggtgtg aagctggcac aggtttacct gcagcgcagc aaccgcacgc tctcgccggg
      121 tcaggacctg tttggcaagc atgtctttgt cgatctctgc aatggcgtgc tgcagaccct
      181 gccgccggca ctgcagcagt ttctggtaca gaccgccctg ctggaaagtt tcagcgcgcc
      241 tctttgcgat tacatgctgc acagtgccca tggcgccgag catatccgcc agttgcagga
      301 actggggttg ttcatcgagg cggacccggc gcaatccgcc ttccgttacc acgccctgtt
      361 tcgccatcat ctgctgcagc ttttccggca gacgccgctg gaacagcagg accgggtgat
      421 tgcgcgggcc tgcacctggc tgatggatag cggtcagcgt gaagcggcct gccggctggc
      481 gcgcgaacac agccagcgcg gctttttcgt tgaagtgctg caacgcagtt ttcacgcctg
      541 gttcaaggcc ggacgtggcg atccggtgtt ttactgggcc cgtgaactgg gtgaaaaggc
      601 gctgctggcg ctaccggaaa cccgtttcgc ctggtgctgg gctctcacca tgttcggccg
      661 ctgggacgat gccgaaagcc tgctggtgcg cacaccggtg tccgccgaga gcgcggacca
      721 gatgcaaccg gatcccgcca gcctggtgct gcaatccatc atccgtctgt tccgttgcga
      781 actggatgac agccagatcg agctgctgca acggctgtac aagctgccgg agctggcccg
      841 ggatctgcgg gcctccatcg ataacatcct cgcccagcat gcaattcacc gctgtcagta
      901 ccaggaggcc tggaagcgcg cgtcccaggc agccctgatg ctggcacaga acggcaatct
      961 gttcggccag accctggccc agtacctgat cgccaatgcc agtttccaga acaatgatat
     1021 ccgtaacgcc cttgccacct gtgaccagta cctggaacag caaccgctgg atgcggatca
     1081 cccctcccgc gccctgctgg caggattccg cgcctacctg ctgtaccaag gcgaacagcc
     1141 gctgcaggcg gagcagttga tacatgacgt gctggccggc agccagccag gctacagcat
     1201 cgacctgcag ttgtttctgt acgtgcccct gatccggctg cgtgcccgcc gtggccagtt
     1261 tgtggcagcc catgaattgc tggaggaact ggaagccgct gccagtgcga gcgcatcgcc
     1321 gctgttccag gcccataccc gctttgaagc gttgcgtctg gcctacgcca agggcaacgg
     1381 caatgaactg cagcgactgg ccgcccagta tggcgtggaa gaagcggcgc ggaaggcgct
     1441 ggatccaaac ctgccattgc agtgggaaac ccgggaatac cggataatgg ccggcataat
     1501 tgtttacatg catcaggaac agtttgaccg ggccagggca ctggcgctgc agctactgca
     1561 cctgaacgtg gaccatggct accccatccg ctttctgccc atcaatatgt acatcgcgta
     1621 tctggatctg tgcctgggcc agatcagctc ggcctaccgt cgcctgaacg acaccctgac
     1681 gcaggcggaa gccaccggca tgctgatggg gctgatcgac gatctgccgg gcctggatga
     1741 catgctgcgg ctggcgctgt cacaccagcg catactgaat ccggagcacg tccgccggat
     1801 acgggcgatg ggcattctgg aactgcaggc cccggcgcag acgccggcac tgacgccgcc
     1861 ggagctggat atcacccgcc tgctgctgca ggggctcaac gtgcacgata ttgccgatca
     1921 tctgcagctg tcgctgacga cggtgcaatg gcatctgcag cgcatcctgt acaagctgga
     1981 tgcgcgccag atcggcgatc tagtgccccg cctgcgtcag ctcgatatcc tgccctcctc
     2041 ataaacggtc cgcatccaac ggccggttca ccgcactttc ttcctgcaag cgcaccagcg
     2101 gctccgcttc cagcagccgt tccgccgtgt acatgatcag ccgtcgcaac cactgatgcg
     2161 ccgcattggg atgcagcagc ggactccagg ccattttcag ttcgaacggg ggaatgtaga
     2221 acggcggctc ggtgatgacg aggcagggat tttccgcctg catgcgggcg acacgggtgg
     2281 gcagtgtcgc caccagatcg ttgttttgcg ccagcaggcc gggcatctgg taatggcggg
     2341 tgaagatggt gatcttgcgc ttcttgccga tccgcgccag tgccgaatcg atccatccca
     2401 gaccgccgga tttttccgga ttgataccga agcccacgcc catgccggtc ttgctcaccc
     2461 agatgtgctg ggccagcaga taggttttca ggttgaagtt gtgcacgatg ggattgtcgc
     2521 ggttgatcag gcaggaaaag ctgtctttcc agataaccgt ctggtggaac gactggggaa
     2581 tttcatcgaa acggttgatg gccatgtcca cccggccctg ctccatgtcc tggtaactga
     2641 cgtcgctggg ggtgagcatg tcgaggatga tgtggggcgc ttcgctgcgg atgtgcttga
     2701 tcagcgcggg gatcagggtc gcctcggcat aatcgctcac catgatgcgg aacacgcgct
     2761 tgctggtgag cggcttgaat tcctggcgcg gttccagcag gtactgggtt tcggccagga
     2821 tctggcgcac cttgggctgc aggtcgagcg cacgctcggt gggcttcatg ccatcgctgg
     2881 agcggatcag cagcgggtcg ttgaacagct cgcgcaggcg cttgaggcca ttgctcatcg
     2941 cgggctgggt gatgcccagc tgctccgccg cccgggtcac gttgagttcc cgcagcaaaa
     3001 catcaaggta taccagcagg ttgagatcga cgcggccgac attcatggga tatatggcaa
     3061 tacctgaaat ataaattcag gaaattatgg cagatctggg ggtcttgttc acggaatctg
     3121 tgttgtccga gaggtgctta gcgtgtctga gcccgttgtt gcaggcgccg cagctcggcg
     3181 tccagcagtg cgggatcatc caacggcagc atggcgcgat agcggggatg cagcagccag
     3241 cgccgcagtg tgcgccattg tccctgtgcc agttgccgct ctgcctcctg ggggaagagc
     3301 ggcaaccagt gggtgtcgcg aatttgcggc ggttgcaccc gctgcaggga cgtaattact
     3361 tcgccggagt gctccgagcc gagaatcagt gtccgcagca gcgcgggcag atcgatgccc
     3421 acctgatccg ccagaacaaa ctgcccgttg gggcgaccgt gctgttccag cgcaaacagg
     3481 ttgccatttt catcccgcat gaaatcggcg ccacccagac cgtggaaacc ggacatctgc
     3541 cccattttct ccagcatccc ctgcatctgc ggcggcgcgc acaaacgcac catactggcc
     3601 gggccaaagc ggccaggcca ggtgcggatt ttttcgaacg ccagccaggc cagcaggcgc
     3661 ccctgttgcc cgatcgcggt
//
LOCUS       DASUEG010000136         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_222408, whole genome shotgun sequence.
ACCESSION   DASUEG010000136 DASUEG010000000
VERSION     DASUEG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222408"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1321
                     /locus_tag="VFX11_02535"
     CDS             77..1321
                     /locus_tag="VFX11_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX4937521.1"
                     /translation="MAFDNIYIIGIGMTPFGRHLDKSLKQLTAAAVDEALRDAQLDRG
                     AVEEIHFGNCVQGYMEGQHMIRGHSIFLEQGFQGIPVFNLESGCASGSMAFHNAVRGL
                     LAGQADVVMAVGAEKMVSTDKDKMFNAFTSAWDVARTDWQMDQIAAMGAGMPIPPGST
                     SPKPYSPFMDVYKGEFLQHVRAYGVTQRQVAAICAKNHRHSVHNPKAQFRVVYDIDQV
                     LAAPAITWPLTLPMCAPISDGAAAVILCNAAGLQRAGLYRQRAIRVLGSVTTSGIERH
                     VNDESRHIVRRAANKLYEQTGLGPQDMHLAELHDATAVGELLETEALGFCGPGEGGTL
                     AESGATTIGGRIPVNTSGGLESRGHPIAATGIAQIHDLVQQLRGEAGARQVSQARLAL
                     QCNVGGFWGVEEASAHVAVLGK"
     gene            1367..>2243
                     /locus_tag="VFX11_02540"
     CDS             1367..>2243
                     /locus_tag="VFX11_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937522.1"
                     /translation="MQFQSTIRRLASVSLLALLSGCTHNMVRDMAGSGMVNFGNEYIT
                     PWFMASDDTDVMCAMGEGMGAMTFPLGPATDPMVPMLTMASGMCADERAKEEELRYIR
                     AMRKNDAEEAQDARTLQKRWSELAARRQYFGYQAVVRYFGEPGEECPDFADRNEQLSY
                     MFGLFGGIQAFQTDLSNGGTAGVPLSVLPKSLKGLDCLKGDEFWGLPQAIKASVDIMK
                     ANIGGEKEALDVGMAQMDAASRIGQKQGVRIVQLVEASLYAAMGNTEKTKQVIREHVK
                     LKNTVAADPELKILDE"
BASE COUNT          494 a          665 c          672 g          412 t
ORIGIN      
        1 gcaaaccgga tttccgtggc tactgacatc catggctact gacttccgca gtgactaaac
       61 aagggcgacc actgacatgg ctttcgacaa catctacatc atcggcattg gcatgacgcc
      121 gtttggtcgc cacctcgaca aatcgctgaa gcagctgacg gctgccgcag ttgacgaagc
      181 cctgcgcgac gcacaactgg atcgcggcgc cgtcgaagaa attcatttcg gcaattgcgt
      241 gcagggctac atggaaggcc agcacatgat tcgcggccac tccattttcc tggaacaggg
      301 ttttcagggt attcccgtgt tcaacctgga aagcggttgc gccagtggca gcatggcatt
      361 ccataacgca gtgcgcggat tgcttgccgg acaggccgat gtcgtgatgg ccgtgggcgc
      421 ggaaaaaatg gtgagcaccg acaaggacaa gatgttcaat gccttcacca gcgcctggga
      481 tgtggcccgc accgactggc agatggacca gattgctgcg atgggtgccg gcatgcccat
      541 tccgccgggc agcacctctc ccaagccata cagtccgttc atggacgtgt acaagggcga
      601 attcctgcag catgtccgcg cgtacggcgt cacccagcgc caggttgccg ccatctgcgc
      661 caagaaccat cgccattccg tccataatcc aaaagcgcag ttccgcgtag tctacgacat
      721 tgatcaggtt ctggccgcac cggcgattac ctggccgctc acgctgccga tgtgtgcccc
      781 gatcagcgac ggagccgccg ccgtgatact gtgcaacgcc gccggattgc aaagagccgg
      841 cctctatcgt cagcgcgcca ttcgcgtgct gggcagcgtg accaccagcg gtatcgagcg
      901 ccatgtcaat gacgaatcgc gccacattgt gcgccgcgct gcaaacaagc tctatgagca
      961 gactggcctt ggcccgcaag atatgcatct cgctgaattg cacgacgcca ccgccgtggg
     1021 tgaattgctg gaaaccgaag cactcggctt ctgtggaccc ggtgaaggcg gcacgctggc
     1081 ggaaagtggc gccacaacca ttggtggccg cattcccgta aatacgtctg gcggactgga
     1141 atcccgtggc catccgattg cggctaccgg catcgcgcag attcacgatc tggtgcagca
     1201 attgcggggc gaagccggcg cacgtcaggt gagccaagct cgcctggcgc tgcagtgcaa
     1261 tgtcggtggt ttctggggag tggaagaggc gtccgcccac gtggcggtgc tgggaaaata
     1321 gaacactcat aacaaataga aactgaacaa tacaaggaag cacaacatgc aatttcaatc
     1381 gaccatccgg cgcctggcgt cggtttcgct gctggcgctg ctgagcggct gcacgcacaa
     1441 catggtgcgg gacatggccg gctccggcat ggtgaatttt ggcaacgaat acattacccc
     1501 ctggttcatg gccagcgacg acaccgacgt gatgtgcgcg atgggcgaag gcatgggcgc
     1561 catgaccttt ccgctgggac ccgccaccga tcccatggtg ccgatgctga cgatggcctc
     1621 cggcatgtgc gccgatgagc gcgccaagga agaagagctg cgttacatcc gcgccatgcg
     1681 caaaaacgac gccgaagaag cacaggatgc ccgcaccctg cagaaacgct ggtccgaact
     1741 ggcggcccgc cgtcagtact tcggctacca ggctgttgtg cgttactttg gtgaacccgg
     1801 tgaagagtgt ccggatttcg ccgaccgcaa cgaacagctc agctacatgt tcggcctgtt
     1861 cggcggcatc caggcattcc agaccgatct gtccaatggc ggcaccgcag gtgttccgct
     1921 gtcagttctg ccgaaatccc tgaaaggtct ggactgcctg aaaggcgatg aattctgggg
     1981 cctgccacag gcaatcaagg cctcggtcga catcatgaaa gccaatatcg gcggcgagaa
     2041 ggaagcactg gatgtaggca tggcacaaat ggacgctgcc tcccggatcg ggcagaagca
     2101 gggcgtgcgc attgtgcagc tggtggaagc ttccctgtat gccgccatgg gcaacaccga
     2161 gaaaaccaag caggtgattc gcgagcatgt gaaactcaag aacactgtag ccgccgatcc
     2221 ggagctgaaa attctcgacg aaa
//
LOCUS       DASUEG010000137         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_7537, whole genome shotgun sequence.
ACCESSION   DASUEG010000137 DASUEG010000000
VERSION     DASUEG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7537"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VFX11_02545"
     CDS             complement(<1..147)
                     /locus_tag="VFX11_02545"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:NF014004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX4937523.1"
                     /translation="MNERGAEKRNRQNLRTGFTTGACSAAAARAAAVGLVTGTVPEQI
                     ECLLP"
     gene            complement(154..840)
                     /locus_tag="VFX11_02550"
     CDS             complement(154..840)
                     /locus_tag="VFX11_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX4937524.1"
                     /translation="MTQNIITEQMTRLGQQIEHESFAVVDAEAGAHAYRAEQWPVVRR
                     MIHATADFEFNGLTRFHPDAIAAGVNALLAGRPVVADVDMICVGVSKPRLAHFGVVAH
                     QFISDSDVIEQSRRADSTRAVYAMKKARQLGLLDGGIVAIGNAPTALLEVIRMIREDD
                     LRPALVIGMPVGFVSAAESKAALEQIDAIPWIITHGRKGGSTLVVAALHALLALAEQR
                     QHQSDAADSL"
     gene            complement(856..1767)
                     /locus_tag="VFX11_02555"
     CDS             complement(856..1767)
                     /locus_tag="VFX11_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEX4937525.1"
                     /translation="MTDVILLVGHGSREQNGNTEIEEFAHQWRARRPRWLIQFCFIEF
                     ADVLLEEGLDRAARSGQRVIVVPLILNAAGHVKMEIPQHIAEARARHPLVRFAYTPHL
                     GASDPILRILKRRLQQGMAALDMPDPCTTGVVVLGRGASDKMANGDVAKMARWLQEDG
                     RHELVDIAFTGITYPRLERVVQRQVLLGMTQIVVLPYYLFTGTLIERIRRQVEHLRLQ
                     YPRVRFACGEYFGFEDEIFQLLEQHVESALRGEEQTLMPCDGCKYREVAEQHGYGHSH
                     HRDHGHDHEPRPVRKPAHPHDHDHAHA"
     gene            complement(1760..2593)
                     /gene="cobM"
                     /locus_tag="VFX11_02560"
     CDS             complement(1760..2593)
                     /gene="cobM"
                     /locus_tag="VFX11_02560"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX4937526.1"
                     /translation="MSEHPLETPSAIAGKVWFVGAGPGDPDLITLKGRDLVARAGAIL
                     YAGSLVAQAATQWASADCEITDSKDMTLEQITAWLMAQAHRHATVVRLQTGDPSLYGA
                     LIEMVQPLDAAGIAIEVVPGVSSAMAAMAAAVESLTLPEVTQTVILTRVEGRTPMPAG
                     ESLAELAQHQCTLCLYLSITLLKKIRSELLQAGWSDDTPVLVVQKASWPGEQKIIRGT
                     LADIRQRCIDADVSSQAMIVVSPTLGARSWSTLTKSKLYDAGFSHRFRRGHPTEQILH
                     D"
     gene            complement(2590..3909)
                     /locus_tag="VFX11_02565"
     CDS             complement(2590..3909)
                     /locus_tag="VFX11_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX4937527.1"
                     /translation="MNVAPQCCRALFIGAPASGQGKTTVTAALARLYRRQGLRVRVFK
                     TGPDFLDPMILEQASGAPVYQLDLWMGGEQHCRALLQRAAAEADVILVEGVMGLFDGE
                     PSGADLAALFGLPVLAVIDASAMAQTFGALAHGLAHYRKHLLFAGVLANRVASPGHTD
                     MLRDSLPADLRWFGALPRDAQQALPSRHLGLHQAAEIADLDARIELAADALAAGAGLD
                     LLPPPVAFGSDNTAGAAAFDCSGLGTPLQGCRIAVAFDAAFSFLYRANLDLLRQLGAD
                     IRFFSPLVDAAVPDADALYLPGGYPELHVHTLADNASMKKSIHFHHARNKPIVAECGG
                     MMLLLETLQNSAGERGDMVGLLPGAAIMQSRLVNLGLHSVALPEGTLRGHTFHHSLTE
                     TTCTPLTHTEGRRGRPEAVYRVGRLHASYLHCYFPSNPLACARLFAA"
     gene            complement(3921..4661)
                     /locus_tag="VFX11_02570"
     CDS             complement(3921..4661)
                     /locus_tag="VFX11_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX4937528.1"
                     /translation="MLKMLSPALFAALLAALVLSLSQALWVSPLILQAEVYESDPAQP
                     VEDVAGHHHDDHAAHEHDESAWAPEDGWQRHAATLASNLVMAVGYGLILCACYAWSFQ
                     TRKKPAGWRAGLAWGGAGYVVFFLAPALGLPPELPGTLAAELEARQLWWLLTLLCTAA
                     GLALLVWARPLWLKVVALLPLVLPHVLGAPHPAHAFALAPATLTWQFIVATSLVNALF
                     WLVLGAISAQLFARARRGDNVALQGIVL"
     gene            complement(4672..4857)
                     /locus_tag="VFX11_02575"
     CDS             complement(4672..4857)
                     /locus_tag="VFX11_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HEX4937529.1"
                     /translation="MSGSTSPYSSLSLPGSTAAVFKPLALLFAGVCLIFLVGFAPVSA
                     LHNAAHDTRHSAAFPCH"
     regulatory      complement(5002..>5219)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1092 a         1767 c         1467 g          893 t
ORIGIN      
        1 gggcagcaga cactcgatct gctccggcac ggtacccgtt accaatccca ccgccgcagc
       61 ccgggcagcg gcagccgaac aggcaccggt cgtaaatccg gtgcgcaggt tctgccgatt
      121 gcgtttttcc gcaccacgct cattcatcgg cgatcagagg gaatctgctg cgtcggattg
      181 atgctgtcgt tgttccgcca gcgccaacag tgcatgcaat gctgccacca ccagggtgga
      241 accgcccttg cggccgtggg taatgatcca gggaatcgca tcgatctgtt ccagtgccgc
      301 cttcgattcc gccgccgaca cgaagcccac cggcatgccg atgacaagtg ccggacgcaa
      361 atcgtcttcc cggatcatcc ggatcacttc cagcagcgcg gtaggcgcat tgccgatggc
      421 aacaatgccg ccatcgagca aaccgagttg ccgcgctttt ttcattgcat agaccgcgcg
      481 cgtgctgtcg gcccgtcgcg attgttcaat cacatcgctg tcgctgatga actggtgggc
      541 gacaacacca aaatgcgcca gccgcggttt cgacacgccc acgcagatca tgtccacgtc
      601 cgccaccaca ggccgccccg ccagcagcgc gttgacgccc gccgcaatcg cgtccggatg
      661 aaaccgggtc aggccgttga attcgaaatc cgccgtcgcg tggatcatgc gccgcaccac
      721 aggccactgc tccgcgcggt aagcgtgggc gcccgcctcg gcatccacca cggcaaacga
      781 ttcgtgctcg atctgctggc ccaggcgggt catctgctcg gtgattatgt tttgggtcat
      841 ggccgtctgc ctcgctcagg cgtgtgcgtg atcgtgatca tgcggatgag ccggcttccg
      901 gactggccgg ggttcatgat cgtgtccatg atcccgatga tgggaatggc cgtagccatg
      961 ctgctccgcc acttcgcggt atttgcagcc gtcgcacggc atcagcgtct gctcttcgcc
     1021 gcgcaacgcg gattccacat gctgttccag caactgaaaa atttcgtcct cgaacccgaa
     1081 gtactcgccg caggcgaaac gcacgcgcgg atattgcagg cgcaggtgtt ccacctggcg
     1141 ccggatacgt tcgatcaaag taccggtgaa caggtaatac ggcagcacca cgatctgcgt
     1201 catgcccagc aaaacctggc gctgcaccac ccgctccaga cgtggatagg taatcccggt
     1261 gaacgcgatg tccaccagtt cgtggcgacc gtcctcctgc agccagcgcg ccattttcgc
     1321 cacatcgccg ttggccattt tgtcggatgc gccgcgcccc agcaccacca cgccggtcgt
     1381 gcacgggtcc ggcatgtcca gcgccgccat tccctgctgc agacggcgtt tcaggattcg
     1441 caggatggga tcgctcgcgc ccaggtgtgg cgtgtaggcg aaacgcacca gcggatgccg
     1501 tgcgcgcgcc tcggcaatat gctgcggaat ttccatcttc acatgaccgg ccgcgttcag
     1561 gatcagcggc accacgatca cgcgctgacc gctccgcgcg gcgcgatcaa gtccctcttc
     1621 cagcagcaca tcggcaaact cgatgaagca gaactggatc agccagcgtg gccggcgcgc
     1681 acgccactga tgcgcgaatt cttcgatttc cgtgttgccg ttctgttcac gggaaccatg
     1741 tcccaccagc aggatgacgt cagtcatgca gaatttgctc cgttggatgg ccgcgccgga
     1801 aacgatggct gaatccggcg tcatacagtt tgctttttgt gagcgttgac cagcttcgtg
     1861 cgcccagcgt cgggctgacc acgatcatcg cctggctgct cacgtcagcg tcgatgcagc
     1921 gctggcggat atccgccagc gtgccgcgaa tgattttctg ctcgcccggc cagctggctt
     1981 tctgcaccac cagcaccggc gtatcatccg accagccggc ctgcagcagt tcgctgcgga
     2041 tttttttcag cagcgtgatc gacaggtaca gacacagcgt gcactgatgt tgcgccagct
     2101 ctgccagcga ttcaccggct ggcatgggcg tgcggccttc cacccgcgtc aggatcaccg
     2161 tctgcgtcac ttccggcaac gtcaggcttt ccaccgctgc cgccatcgcc gccatcgccg
     2221 acgacacgcc cggcactact tctatcgcga tgccggccgc atccagtggc tgcaccatct
     2281 cgatcaacgc gccatacagc gatggatcgc cggtctgcag tcgcaccacg gtggcatgac
     2341 gatgtgcctg tgccatcagc caggccgtga tctgttccag cgtcatgtct ttggaatcgg
     2401 taatctcgca atccgctgaa gcccactgcg tcgccgcctg cgccaccagc gaaccggcat
     2461 acagaattgc gccggcgcgg gccactagat cacggccttt caatgtgatc agatcaggat
     2521 cgcccgggcc ggcgccaaca aaccagactt tgccggcgat ggctgacggc gtttccaaag
     2581 gatgctcgct catgcggcaa acaacctcgc acatgccaat ggattcgacg gaaaatagca
     2641 gtgcagatac gacgcatgca ggcggccgac acggtacacc gcctccggcc ggccacggcg
     2701 accttcggtg tgcgtcagcg gcgtgcatgt cgtctcggtc agcgaatgat gaaaggtgtg
     2761 gccgcgcaga gtgccttccg gcagggccac cgagtgcaat cccagattca ccagccttga
     2821 ctgcatgatt gcagcacccg gcagcaaacc caccatgtcc ccgcgttcgc ccgcgctgtt
     2881 ctgcagcgtt tccagcaaca gcatcatgcc gccacattca gcgacaatcg gtttgttgcg
     2941 tgcatgatga aaatggatgg attttttcat gctggcattg tctgccagcg tatgcacatg
     3001 cagttccgga taaccgccag gaagatacag cgcgtcagcg tctggcacgg cggcatccac
     3061 caggggcgag aaaaaccgga tgtccgcgcc cagttgccgc agcagatcca gattggcgcg
     3121 atacaggaat gaaaatgcag catcgaacgc caccgcaatg cggcatccct gcaacggcgt
     3181 acccaatccg ctgcaatcga acgcagcggc gcccgctgtg ttatcgcttc caaacgccac
     3241 cggcggcggc aacagatcca gccctgcacc ggccgccagc gcatccgcgg ccaactcgat
     3301 ccgcgcatcc agatcggcga tctccgccgc ctgatgcaaa cccagatggc gcgaaggcag
     3361 tgcctgttgc gcatcgcggg gcaacgcgcc gaaccagcgc aaatccgccg gcaaactatc
     3421 gcgcaacatg tccgtgtgac cggggctggc aacccggttg gccagcacgc cggcaaacag
     3481 cagatgcttg cggtaatggg ccaggccatg tgcgagcgcc ccaaacgtct gggccatggc
     3541 ggaagcatca atcactgcca gcaccggcaa accgaacaac gctgccagat cagcaccgga
     3601 aggttcgcca tcaaacaacc ccatgacgcc ttccaccaga atcacatcgg cttccgccgc
     3661 cgcccgctgc aacaacgcgc gacaatgctg ctcaccaccc atccacagat ccagctggta
     3721 cacgggcgcg ccagacgcct gctccagaat catcggatcg agaaaatccg ggccggtctt
     3781 gaacacgcgc acccgcaaac cctgccggcg ataaagccgc gccagcgccg ccgtcaccgt
     3841 ggttttaccc tgaccggaag ccggagcgcc gataaacagc gcacgacagc attgcggtgc
     3901 aacattcatc tcggcacgcg tcacaacacg ataccctgca acgcaacatt gtcaccgcgc
     3961 cgggcacgtg caaacaattg tgccgaaatc gcgcccagca ccagccagaa cagcgcattc
     4021 accaacgatg tcgccacgat gaattgccac gtcagcgttg cgggcgcgag cgcaaacgcg
     4081 tgcgccggat gcggcgcacc cagcacatgc ggcaacacca gcggcagcaa cgccacgact
     4141 ttcagccaca gcggacgggc ccacaccagc aacgccaacc ccgctgccgt gcacagtaaa
     4201 gtcaacagcc accacagctg tcgcgcttcc agttctgccg ccagagtgcc cggcaattcc
     4261 ggcggcaatc ccagcgccgg agccagaaaa aacaccacat aacccgcacc gccccacgcg
     4321 agacccgcac gccagcctgc cggttttttt cgcgtttgaa aggaccacgc ataacacgcg
     4381 cacaggatca ggccatatcc cactgccatc accagattcg acgccagcgt ggcagcatga
     4441 cgttgccagc cgtcctccgg tgcccacgcc gattcgtcat gctcgtgcgc cgcgtgatcg
     4501 tcatgatggt gccccgccac gtcttcgacc ggctgcgccg gatcgctttc atacacttcc
     4561 gcctgcaaaa tcagcggcga aacccacagc gcctgcgaca gcgacaacac cagcgcagcc
     4621 agcagcgccg caaacagcgc gggcgacagc atcttcagca tcgtcatggc ctcagtggca
     4681 gggaaaggcc gccgaatgac gggtgtcatg cgcggcatta tggagagcgg acaccggcgc
     4741 aaagcccacc agaaaaatca ggcagacacc ggcaaacagc agcgcgagcg gcttgaaaac
     4801 ggcagcagtg gacccgggga gggaaaggga tgaatagggc gaagtggaac cggacatggg
     4861 attttccttc tgcgaatcag cgtgagaacg gcaagctgca gaaaggaaaa ccagaacaga
     4921 tcaactcagg ggatgcagaa aaagcgtcga gcgctgcagg aatccgagat tcaccggtct
     4981 ggcccgccca ccgcaggaca tttaccatct ttcagctcag gccggtatcc agactcgcaa
     5041 gcccgtcatc cttcgatgac ggcaaaacag cgccttccca tgccgcacat gacacagtgg
     5101 ctttgtgctg tttctgactc acttactgtt gcgggggcag taccggaatt gccgcaatga
     5161 tgcagcgcac cggtttcccg tttaacctct acgtcgaacg cttcgaggca cctgaaacg
//
LOCUS       DASUEG010000138         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1267140, whole genome shotgun sequence.
ACCESSION   DASUEG010000138 DASUEG010000000
VERSION     DASUEG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267140"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1195)
                     /locus_tag="VFX11_02580"
     CDS             complement(164..1195)
                     /locus_tag="VFX11_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4937530.1"
                     /translation="MTKTAAPTQLPPLVDQPLAPELLQQLQQASALKKWPLAKLISLF
                     ENRNPDAAQIRHDIARHIQQHPQDFPRNARVIGFTGTPGAGKSSLIAELCRKLLTLND
                     KIAIAVLAVDPSSQISGGSILGDRTRMHYGKKEERLFFRSQASNLELGGVSEATFQAT
                     RLLRHLFDLIIIETVGIGQNEVDIQFLTDHTFLVMQPLAGDQIQFMKSGIMEIPDSFI
                     VNKCDEERLARTRYHMLRSSLKQARLVDLHAQDDYQNAARDQIFLTSALKGLGIDTLA
                     NAVLNIPQRRTAEHCESCYLRKGVLRRYGEFGLRILKHEMNLLDTATTFEEKELAAFA
                     TIQRHIIPR"
     gene            complement(1212..>2330)
                     /locus_tag="VFX11_02585"
     CDS             complement(1212..>2330)
                     /locus_tag="VFX11_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX4937531.1"
                     /translation="RDARCRALQLPAWNEALSLPRPWDQQWSLRLQQILAYETDLLEY
                     PDLFAGSPVVQSKVEELKAQARAEIQKILDAGGGVEAIKTGYMKSQLVISQAERMAKI
                     NTGEQVVVGKNRWMDGLASPLVGGNDGGVFKVDPESANETVHALEETLRKRDAAKVKA
                     CLDRLREDAKAGRNLMEASIACAHARVSTGEWAGTLREVFGEYRPPTGVEGQTLSLEN
                     EKLQQVRAKVQQFMDQHGHRPRIVVGKPGLDGHSNGAEMISVAARHAGFDVIYFGIRL
                     SAVDIVQSAIEENVDVIGISLLSGSHNEIIAQLMNELEQEGARDAIPVVLGGIIPQND
                     IPALTALGIRAVFTPKDYDLMDIMNKIMDIVLEHKARG"
BASE COUNT          437 a          682 c          692 g          519 t
ORIGIN      
        1 gaaaaaaatg ccgatcttgc tcatgacgtc agccccgttt gtaggatgtg cgacaacgct
       61 ctggcgtgcg tagcgccaca agacgttttt tgtgggaagt ccgacaggaa agcgggcccc
      121 ttgcagggta attgcaacag gcataccaga ggggcgggtg ttgtcagcgc ggaatgatgt
      181 ggcgctggat cgtggcgaat gcggccagtt ctttttcttc aaacgtggtg gcggtgtcca
      241 gcaggttcat ctcgtgcttg aggattctca gaccgaactc gccataacgc cgcagcacac
      301 cctttctcag atagcaggat tcgcagtgct ccgccgtgcg gcgctgggga atattcagca
      361 cggcgttggc cagggtgtcg atgccgagac ctttcagcgc gctggtgaga aaaatctggt
      421 cccgcgccgc gttctgataa tcgtcctggg cgtgcaggtc caccagccgc gcctgcttca
      481 gcgacgaacg cagcatgtgg tatctggtgc gggccagccg ttcctcgtcg catttgttga
      541 cgatgaagct gtccgggatt tccatgatgc cgcttttcat gaactggatc tggtcacccg
      601 ccagtggctg cattaccagg aatgtgtgat cggtaagaaa ctggatatcg acttcattct
      661 ggccgatgcc cacggtttcg ataatgatca gatcgaacag atgccgtagc aaacgcgtgg
      721 cctgaaacgt ggcttcactc acaccaccca gttccagatt cgatgcctgc gagcggaaga
      781 aaagccgttc ttccttcttg ccgtaatgca tgcgggtgcg gtcgcccaga atcgagccgc
      841 cggaaatctg gctggacggg tccaccgcca gtacggcgat ggcgattttg tcattcagtg
      901 ttaacagctt gcggcacagt tccgcaatca gcgaggattt acccgcaccc ggggtaccgg
      961 taaaaccgat tacgcgggcg ttgcggggaa aatcctgcgg atgctgctgg atgtggcgcg
     1021 cgatatcgtg gcggatctgc gccgcatcgg gattcctgtt ttcaaacagg ctgatgagct
     1081 tggccagcgg ccattttttc agggcgctgg cctgttgcag ttgttgcagc agctcgggtg
     1141 caagcggctg gtccactaac ggcgggagct gtgttggcgc tgctgtttta gtcatgcaag
     1201 gtggcgcccg gtcagccgcg cgccttgtgt tccagcacga tgtccatgat cttgttcatg
     1261 atgtccatca gatcatagtc tttcggcgtg aacacggccc ggatgcccag cgccgtcaga
     1321 gcgggaatgt cattctgcgg aatgatgccg ccgagcacca ccggaattgc atcgcgggca
     1381 ccttcctgct ccagttcgtt catcagctgt gcgatgattt cattgtgcga gccggacagc
     1441 agggaaatac caatcacatc cacgttttct tcaatggccg actgcacgat atccaccgcg
     1501 ctcaggcgga tgccgaaata gatcacgtcg aaaccggcat gacgcgcggc cacggaaatc
     1561 atctcggcgc cattggagtg gccatccaga cccggcttgc cgacgacgat acgcgggcgg
     1621 tgaccgtgct ggtccatgaa ctgctgcacc ttggcgcgca cctgctgcag tttttcgttt
     1681 tccagcgaca gcgtttgtcc ttccacgccc gtgggcgggc gatattcacc gaacacttcg
     1741 cgcaatgtgc cggcccattc gccggtggaa acccgggcat gggcgcaggc gatggacgct
     1801 tccatcagat tgcggccggc tttggcgtct tcacgcagcc ggtccaggca ggcttttacc
     1861 ttggcggcat cgcgtttgcg aagggtttct tccagcgcgt gcacggtttc gttggcggat
     1921 tccggatcga ccttgaacac accgccgtcg ttaccgccca ccagcggcga cgcaaggccg
     1981 tccatccagc ggtttttgcc cacgaccacc tgttcgcctg tattgatttt ggccatgcgc
     2041 tcggcctgcg aaatcaccag ctgggatttc atgtagccgg tcttgatagc ttccacgccg
     2101 ccgccagcat cgagaatttt ctggatttcg gcgcgggcct gcgctttcaa ctcttccact
     2161 ttcgattgca ccaccggcga gccggcgaac agatccgggt attccagcag atccgtttcg
     2221 taagccagaa tctgttgcag gcgcaacgac cactgctgat cccacggccg cggcagcgac
     2281 aacgcttcat tccatgccgg caactgcagc gcacggcaac gggcgtcacg
//
LOCUS       DASUEG010000139         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1907071, whole genome shotgun sequence.
ACCESSION   DASUEG010000139 DASUEG010000000
VERSION     DASUEG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907071"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2011)
                     /locus_tag="VFX11_02590"
     CDS             complement(<1..>2011)
                     /locus_tag="VFX11_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4937532.1"
                     /translation="VAVLDRVFGEIVRRHEVLRTNFAENDGVPQLCVREPGPWHSTLT
                     DLSGLDKTAQQKEVERLVDQDANVTFSLANDPLFTTRIVKLAAQEHILLLNMHHIISD
                     GWSLTVLVQEIQALYLAFAAGRASPLPELPIQYSDFAVWQRHWLQGDTLDKLRSYWKD
                     ALNGAPDVLRMPTDKPRPKIQTFNGAHFPLSLGPALAGKVKRYCEQHDLTPFMVLMGA
                     YHVLLSRYSNQQDICVGIPIAGRNRAEIEGLIGFFVNGLVFRSRLDGNPSVEQYFRQI
                     KQVALGAYAHQDMPADLLLDAIKMERSADTSPGAQVGFALQNVAETDVQASMAGLSIE
                     TVPREHKTAKYELSLILQEGAGDIAGVMEYNTDLFVAGTIERMLQHYRHILEQMLDQS
                     ELLLDSIHVVELQDLYGLLKQNPADCELQRLSPMQRDMYLDSLLEPDTLKNSLGYHFI
                     TDGEFDVALWQRASQQLVDSQPMLRAQLLRADIPYADVAYLRIAKRKQVHVAFEDWSA
                     RSTSDEAAAAFAQGKIWQPYDIHGELSQYWVYKLDRNRHLVIFRMNHILLDGAGMAVH
                     LLNSIAAAHSIKSGQPYVPAPVIYPDYVQDNTRRTDTTEVIAHWREVSRAVEALDFPL
                     PPQARQTAGTGRRVEKQVRLDGAHWAAVQQYCEQQRITPSLYFKAL"
BASE COUNT          377 a          621 c          609 g          404 t
ORIGIN      
        1 acagcgcctt gaaatacagc gatggcgtga tgcgctgctg ttcgcaatac tgttgcacgg
       61 cggcccagtg ggcaccgtcc agccgcacct gtttttcgac acggcggccg gtgccggccg
      121 tctgtctggc ctgcggcggc agcgggaaat ccagcgcttc caccgcacgg ctcacttcgc
      181 gccagtgggc gatgacctcg gtggtgtcgg tgcgacgggt gttgtcctgc acatagtccg
      241 gatagatcac cggcgccggc acatacggct ggcccgactt gatgctgtgc gccgccgcaa
      301 tcgagttcag cagatgcacc gccatgcccg cgccatccag caggatgtgg ttcatgcgga
      361 aaatgacgag gtgacgattg cgatccagct tgtaaaccca atattgcgac agttcgccgt
      421 gaatgtcgta cggctgccag attttgcctt gcgcgaacgc cgcggccgct tcgtcactgg
      481 tgctgcgagc agaccagtcc tcgaaggcaa cgtgcacctg tttgcgtttt gcgatccgca
      541 gataggccac atccgcatac ggaatatcgg cgcgcagcag ttgcgcccgc agcatgggct
      601 ggctgtccac cagctgttgc gacgcccgct gccagagcgc gacatcgaat tcgccgtcgg
      661 taatgaaatg gtaacccagg ctgttcttga gtgtgtccgg ttccagcagc gaatccagat
      721 acatgtcgcg ctgcatcggc gacaggcgct gcagttcgca gtcggcgggg ttctgtttga
      781 gcagtccgta cagatcctgc agctccacta catgaatcga atccagcagc agttccgact
      841 ggtccagcat ctgttccaga atgtgccggt aatgctgcag catgcgttcg atggtgcccg
      901 ccacaaacag atcagtgttg tattccatca cgccggcaat atcacccgcg ccttcctgca
      961 ggatcagcga cagttcgtat ttcgcggttt tgtgttcgcg cggcaccgtc tcgatactga
     1021 ggccggccat gctggcctgc acgtcggttt cagctacgtt ctgcagcgca aaacccacct
     1081 gggcaccggg cgaggtgtca gcgctgcgtt ccatcttgat ggcgtccagc agcagatcgg
     1141 ccggcatgtc ctgatgggcg taggcaccca gcgctacctg tttgatctgg cggaaatact
     1201 gctccaccga tggattgcca tccagacggg aacggaatac cagaccgttg acgaagaagc
     1261 cgatcagccc ttcgatctcg gcgcgattgc ggccggcaat gggaatgccg acgcagatat
     1321 cctgctggtt gctgtaacgg ctgagcagca cgtgatacgc acccatcagc accatgaacg
     1381 gcgtgaggtc gtgctgctcg cagtaacgtt tcaccttgcc ggccagcgcg gggcccagcg
     1441 acaacgggaa gtgagcgccg ttgaacgtct ggattttcgg gcgcggcttg tcggtgggca
     1501 tgcgcagcac atcgggcgcg ccgttcaatg cgtctttcca gtagctgcgc agtttgtcca
     1561 gcgtgtcgcc ctgcagccag tggcgctgcc acacggcaaa atcgctgtac tggatcggca
     1621 gttccggcaa cggcgaggcg cggccagcgg caaacgccag atacagcgcc tggatctcct
     1681 gcaccagcac cgtgagggac cagccgtcgg aaatgatgtg gtgcatgttc agcagcagga
     1741 tatgttcctg cgccgccagt ttcactatgc gggtagtaaa cagcggatcg ttcgccagac
     1801 tgaaggtgac attggcgtcc tgatccacca gccgttccac ttccttctgc tgcgccgtct
     1861 tatccaggcc ggacagatcc gtcagggtgg aatgccaggg gcccggctca cgcacgcata
     1921 actgcggcac gccatcgttt tccgcgaaat tggtgcgcag cacttcatgc cggcgcacga
     1981 tctcgccaaa cacgcggtcc agcaccgcca c
//
LOCUS       DASUEG010000140         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1574193, whole genome shotgun sequence.
ACCESSION   DASUEG010000140 DASUEG010000000
VERSION     DASUEG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574193"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFX11_02595"
     CDS             <1..1215
                     /locus_tag="VFX11_02595"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4937533.1"
                     /translation="KTFEDAKGDVWRGIEVVEHAANIPSLLMGETVENVATNIDSYSI
                     TQPLGVCAGITPFNFPAMIPLWMFPLAIACGNTFILKPSEQDPLTPTRLAELFLEAGA
                     PKNVLQVVHGRKQQVDTLLTHPDIKAISFVGSVPVGQYIYTTGTANMKRVQAFAGAKN
                     HMVVMPDANKQQVINNIVGASVGAAGQRCMAISVAVLVGDAKDWINDIKQTLESVRPG
                     VWNDPKAAFGPLISPQARNRVLKLIEQGKAEGATCLVDGSACVVEGFPDGNWVGPTLF
                     SNVTPEMSIYREEIFGPVLCIVTMDTLEDAIEFINNHPCGNGTSIFTASGAAARKYQH
                     EIEVGQVGINVPIPVPLPFFSFTGWKASFYGDQHAYGKQAVRFYTETKTVTARWFDSD
                     IPTGPNMTIHLK"
     gene            1278..2438
                     /locus_tag="VFX11_02600"
     CDS             1278..2438
                     /locus_tag="VFX11_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937534.1"
                     /translation="MNFNLSDEQQAFQDTARAFARAELMPNAAQWDAEAYFPVEVIRK
                     AGAMGFCGIYSPESAGGMGLSRLDAHLIFEQLAYGCTATTAYITIHNMSTWMLTTFGS
                     EELVAQWGPDLTSGEKLASYCLTEPNAGSDAASLKTTARRDGDYYVLNGSKCFISGAG
                     STDVLILMARTGGEGAKGISCLLVPADLPGIEYGRKEEKMGWNCQPTRTISFANVRVP
                     VSNRVGNEGDGFKFAMKGLDGGRINIATCSLGTAQAALDTAQRYLNERKQFGRALADF
                     QALQFKLADMATDLVAARQMVRLAACKLDENDPEASLYCAMAKRFATDAGFAICNDAL
                     QIHGGYGYIREYPLERFVRDTRVHQILEGTNEIMRHIIARRLLLEGATEILQ"
     gene            2435..>2644
                     /locus_tag="VFX11_02605"
     CDS             2435..>2644
                     /locus_tag="VFX11_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4937535.1"
                     /translation="MTQAVLFETLATTGGRKIGVATLNAEKKLNSLSVEMVELLRPQL
                     QQWEQDDSIVCVWLQGAGEKAFCAGG"
BASE COUNT          570 a          724 c          819 g          531 t
ORIGIN      
        1 aagacctttg aagatgcgaa gggcgatgtc tggcgcggta tcgaggtggt ggagcacgcc
       61 gccaatattc ccagcctgct gatgggcgag acggtggaga acgtggccac caacatcgac
      121 tcctattcca ttacccagcc gttgggtgtg tgcgcgggca tcacgccgtt caacttcccc
      181 gcgatgattc cgttgtggat gtttccgctg gcgattgcct gtggcaacac cttcattctg
      241 aaaccgtcgg agcaggaccc gctgacgccg acccgtctgg cggaactgtt tctggaagcc
      301 ggtgcgccga aaaatgtgct gcaggtagtg cacggtcgca agcagcaggt ggatacactg
      361 ctgacccatc cggatatcaa ggcgatttcg ttcgtcggct ccgtgccggt cggccagtac
      421 atctacacca ctggcaccgc gaacatgaag cgggtgcagg cgtttgccgg cgcgaaaaac
      481 cacatggtgg tgatgccgga tgccaacaag cagcaggtga tcaacaatat cgtcggcgcg
      541 tcggtgggtg cggctggtca gcgttgcatg gcgatcagcg tggcggtgct ggtgggcgat
      601 gcgaaagact ggatcaatga catcaagcag acgctggaaa gcgtgcgtcc cggcgtgtgg
      661 aatgatccga aagcggcgtt tggcccgctg atcagcccgc aggcccgcaa ccgcgtgttg
      721 aaactgatcg agcagggcaa ggctgaaggc gccacctgtc tggtggatgg ttctgcctgt
      781 gtggtggaag gctttccgga cggcaactgg gttggcccga ccctgttcag caacgtgacg
      841 ccggaaatga gcatctaccg cgaggaaatc ttcggcccgg tgttgtgcat cgtcaccatg
      901 gacacgctgg aagacgcgat tgagttcatc aacaatcatc cctgcggcaa cggtacttcc
      961 attttcaccg ccagcggcgc cgctgcccgc aaataccagc acgagattga agtggggcag
     1021 gtgggcatca acgtgccgat tccggtgccg ctgccgttct tctccttcac cggctggaag
     1081 gcgtctttct acggcgacca gcatgcctac ggcaagcagg cggtacgttt ctacactgaa
     1141 accaaaaccg tcaccgcgcg ctggttcgac agtgatattc ccaccggtcc gaacatgacc
     1201 attcacctga aataacaaca gcatttgaaa taacaacagc acctgaaaca acaataaatc
     1261 cgaaatagtg gcgcgccatg aatttcaatc tgagtgacga acagcaggcg tttcaggata
     1321 cggcccgggc ctttgcccgc gccgaactga tgccaaacgc ggcgcagtgg gatgcggaag
     1381 cgtattttcc ggtggaggtg attcgcaagg ctggcgcgat gggcttttgc ggcatttact
     1441 cgccggaaag tgcaggtggc atggggctgt cgcggctcga tgcccatctg attttcgaac
     1501 agctggccta cggctgcacc gccaccaccg cgtacatcac cattcacaac atgagcacct
     1561 ggatgctgac caccttcggt tcggaagaac tggttgcgca atgggggccg gatctgacca
     1621 gcggtgaaaa gctggcgtcc tattgtttga cggaacccaa tgccggatcg gatgcggcgt
     1681 cgctgaaaac caccgcgcgg cgcgacggcg attattacgt tctgaatggc agcaagtgtt
     1741 tcatttccgg cgccggttct acggatgttt tgattctgat ggcgcgcacc ggcggtgaag
     1801 gcgcaaaagg catttcctgt ctgctggtgc cggcggatct gcccggcatt gaatacggac
     1861 gcaaggaaga aaaaatgggc tggaactgcc agcccacccg cacgatttcc ttcgccaatg
     1921 tgcgtgtgcc agtgagcaat cgtgtcggca acgaaggtga cggtttcaaa ttcgccatga
     1981 aaggactgga cggtggccgc atcaatatcg ccacctgctc gctgggaacg gcgcaggcgg
     2041 cactcgacac ggcgcaacgc tatttaaatg aacgcaagca attcggtcgt gcgctggcgg
     2101 attttcaggc cctgcaattc aagctggcgg acatggctac ggatctggtt gctgcgcggc
     2161 aaatggtgcg gttggcagcg tgcaagctgg atgaaaacga tcccgaagcc tcgctgtact
     2221 gcgccatggc caaacgtttc gccactgacg ccggctttgc catctgcaac gacgccctgc
     2281 aaatccacgg cggttacggt tatattcgcg aatatccgct ggagcgtttc gtgcgcgaca
     2341 cgcgggttca tcagattctg gaaggcacca acgaaattat gcgccacatc atcgcgcgcc
     2401 ggttgttgct ggaaggtgcg acggagattt tgcaatgacg caggcagtat tgtttgaaac
     2461 actggcgacc accggcggca gaaaaatcgg cgtggccacg ctgaatgctg agaaaaaact
     2521 gaattccctg agtgtggaaa tggtggagct gttgcggccg caattgcagc agtgggagca
     2581 ggacgacagc atcgtctgcg tctggctgca gggtgccggg gaaaaagcgt tttgtgccgg
     2641 cggc
//
LOCUS       DASUEG010000141         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_818617, whole genome shotgun sequence.
ACCESSION   DASUEG010000141 DASUEG010000000
VERSION     DASUEG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818617"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1041
                     /locus_tag="VFX11_02610"
     CDS             82..1041
                     /locus_tag="VFX11_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937536.1"
                     /translation="MQTSNRQWQKQGQIYVPAGDKPWSRSHAQLPLVQKRDSRWRIYF
                     ATRNDAGQSNISFLETAAGNPLDILTIHDGVLLPFGELGTFDESGIMPLSLVRADDGR
                     WHLYYAGWTQRKTIPYHNSIGLAVSDDDCRSFHKLYQGPLLDARRDEPFFTGTAHVRI
                     ESGLWRMWYQSCTGWVRQGQAIEPLYHIKYAESKDGIEWKREGVVAIDYRDKEEGGIC
                     SATINARGDGYEMWYCHRNAFDYRTNPRNSYRIGYATSQDGIVWQRQDSQAGIDVSPE
                     GWDSTMIAYPHFVRDRDTDYLFYNGNGFGASGFGVATRAGERK"
     gene            1038..1787
                     /locus_tag="VFX11_02615"
     CDS             1038..1787
                     /locus_tag="VFX11_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937537.1"
                     /translation="MIQYPVSGLALFQSSLQQALQTDMLDTIAAHIRKGTVVILKNAI
                     PAERALHLRDSIVAWRNQREVVSFSVNTNAPGINFHRIDAHPEKSQLPHIFHQHGFGQ
                     TNSLTPDFSSELLAVANPMLNLQNAVAHTRYSFSMPEVRIKALQYPCGGGFLMKHTHP
                     IDPQGVGLILALSKSGSDFGKGGTTFVTPAGFVDTSAWHDAGDLILFRYDLEHAVGPV
                     DSDSVLDWNSDKGRWTLVLELLSTHNRSEAA"
     gene            1784..2812
                     /locus_tag="VFX11_02620"
     CDS             1784..2812
                     /locus_tag="VFX11_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4937538.1"
                     /translation="MTEHHFSTEQVVCPQCGSQRWRKLTSQWLCRNGHVIPTSAGLPD
                     FSAGGTLAAQDRKLRDRLYNGLLGRYYGFMMPLLSMPARPIMQSLSQWAVFFIAWIVL
                     IDLLWGMTVAFSEGHTIIGGLLLALLLADGVLLYRHSYLFWLLLVAIPVKLILLLRPY
                     KPAETFPDVHRRWVNALKDAGCEQVLDVSTGTCNSLLRHGWTGLGAQLHGVDLSTTML
                     LQGADNAAAAGIPIQLYVADAQAMPFPDACMDLVLNYGALNGYQDQGRALTEMARVLK
                     PGGVLVCLDEQLYDRAGKLEKLYFERVLASHDRIMRFPRESVPVALDLVELHQIYQFY
                     YLAIMRKS"
     gene            2815..4281
                     /locus_tag="VFX11_02625"
     CDS             2815..4281
                     /locus_tag="VFX11_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937539.1"
                     /translation="MSESRNFYVYIDGKMRDWIIDEFDQGKDLLNIKKQIRARTNLSV
                     DMDQIREAIVLSYVDSGESEKAFQLACAFRDQSPTANIHNTVIKCMLSSWEYSQQLFL
                     DESRKWAALYANERMGKFAGYKNAKPRIGFVCDYGSTVFGENAIFPLCHALSHSGFDV
                     YYYNFERNVFSISDNSINILNGYDLSSGALSERIKKDKIDILVDLNGRLREHHRLGVF
                     AQRSAPVQVNYFNLVGTMGMQNYDYIVVDEMQVPRSDEQYYTEKALHLPCGVNGAYAF
                     KRDVDIAVPDRGVGVPFTFASFNAFFKVNAVLLETWAEILRRVPDSRLVIKCHETNRN
                     RVVKKIARVFSRAGIDLGRILIEGWSSLLHLRRQYAEVDLCLDTFPYSGGSTTLNALW
                     QGVPVLTWCGEGWRARTSASMLQAAGVGEMVVSDRKDYVDAAVEFARDFRRSSDMRHF
                     LKANIGSNAYFQPERVYGELAVVLKAIVRREDETAHAM"
BASE COUNT          992 a         1088 c         1329 g         1053 t
ORIGIN      
        1 gcgttctggc agtgaatttg cactggttgt cagatcagtg acgcaagcca gcgtttcgtc
       61 aaatttatgg cgagtggaat catgcagacc agcaaccggc agtggcaaaa gcagggacag
      121 atctacgtac cggcaggcga caagccctgg tcgcgcagcc atgcccaatt gccactggtg
      181 caaaagcggg acagccgctg gcgcatctat ttcgccaccc gcaatgatgc cggccagagc
      241 aatatcagct ttctggaaac cgccgccggc aatccgctcg acatcctgac gatccatgac
      301 ggcgtgctgc tgccgttcgg tgaactgggc acctttgatg aaagcggaat tatgccgctg
      361 tccctggtgc gtgcggacga tggccgctgg catttgtact atgccggctg gacccagcgc
      421 aaaaccattc cctatcacaa cagcatcggg ctggccgtaa gcgatgatga ctgccgcagt
      481 tttcacaagc tgtaccaggg cccgctgctg gatgcccggc gcgacgagcc cttctttacc
      541 ggcacggcgc atgtgcgtat tgaaagcggt ctttggcgca tgtggtatca gagttgtacc
      601 ggctgggtga ggcaggggca ggccatcgaa ccgctgtatc acatcaagta cgcggaatcg
      661 aaagacggca tcgaatggaa gcgggaaggt gtcgttgcga ttgactaccg cgacaaggaa
      721 gaaggtggca tctgcagcgc taccatcaat gcccgcggtg acggctatga aatgtggtac
      781 tgccaccgca atgccttcga ttaccgcacc aatccccgta acagttatcg tatcggctat
      841 gccacttcac aggatggaat tgtgtggcaa cgccaggatt cacaggcggg catcgatgtg
      901 tcgccagaag gttgggatag caccatgatt gcctatcccc attttgttcg cgaccgggac
      961 actgactacc tgttctataa cggcaacggt tttggcgcca gtggctttgg cgtggcgacc
     1021 cgcgcaggag agcgaaaatg attcaatatc ctgtcagtgg actggcgttg ttccagtcct
     1081 ccctgcaaca ggcattgcag accgatatgc tggacaccat tgcagcccat attcgcaaag
     1141 gaaccgtggt aatcctgaag aacgcgattc cggcagagcg ggccctgcat ctacgtgaca
     1201 gcattgtggc gtggcgtaac caaagggagg ttgtgagttt ttctgtcaac accaatgcgc
     1261 ccggcatcaa ttttcaccgc attgatgccc acccggaaaa atcccagcta ccacatattt
     1321 ttcaccagca tggcttcggg caaaccaatt cactgacgcc ggatttttcc agcgagttgc
     1381 tggcggttgc caacccgatg ctgaacctgc aaaacgcggt ggcacacacc cgctacagtt
     1441 tcagcatgcc ggaagtgcgg atcaaggcgc tgcaatatcc gtgtggcggt ggcttcctga
     1501 tgaaacacac ccacccgatt gacccgcaag gtgtcggcct gatcctggcc ctttcgaaaa
     1561 gcggcagtga tttcgggaag ggcggcacta cctttgtcac gccggcagga tttgtcgata
     1621 ccagcgcgtg gcatgacgcg ggtgacctga ttctgttccg ctatgatctg gaacatgcgg
     1681 tgggccccgt agacagtgac tccgtgctgg actggaacag cgataaaggg cgctggacgc
     1741 tggtacttga actgctgagc acccacaacc ggtcggaggc tgcgtgacag aacatcattt
     1801 cagtaccgag caggtggtct gcccgcagtg cggttcgcag cgctggcgta aactgacatc
     1861 gcaatggcta tgcagaaacg gccatgtcat tccaaccagt gccggattgc cggatttttc
     1921 agccggcggc acgctggcgg cacaagacag aaaattacgg gaccggctgt acaacggatt
     1981 gctgggccgc tattacggct tcatgatgcc gctgctgtcg atgccggcgc gcccgattat
     2041 gcagtcactg tcgcagtggg cagtcttttt tattgcctgg attgtgctga tcgatctgct
     2101 gtggggaatg acagtcgcat tcagtgaggg ccataccatc attggcggtc tgctgctggc
     2161 gctgctgctt gccgatggtg tgctgctcta tcgtcattcc tatctgttct ggctgttgct
     2221 ggtggcgatt ccggtcaagc tgatactgct gttgcggccg tacaaacctg cagaaacctt
     2281 tcccgatgta caccggcgct gggtcaatgc attgaaggat gctggctgcg agcaggtgct
     2341 ggacgtttcc accggcacct gcaattcatt gttgcgacat ggctggaccg gcctgggcgc
     2401 acaactgcac ggcgttgatc tttccaccac catgctgttg caaggggccg acaatgcggc
     2461 tgcggccggt attccaatcc agttgtatgt ggcagatgcg caggcaatgc cgtttcccga
     2521 tgcctgcatg gatctggttc tgaattatgg ggcattgaac ggctaccagg atcagggcag
     2581 ggcgctgacg gaaatggcgc gtgtgttgaa gccgggcggt gttctggtat gtctggatga
     2641 acagttgtat gaccgggctg gaaaactgga aaagctgtat tttgaaaggg tgctggcgtc
     2701 gcacgacagg atcatgcggt ttccccggga gtcagttcct gttgcgctgg atctggtgga
     2761 gctgcatcag atttaccagt tctattatct ggcgatcatg agaaagagct gagtatgtca
     2821 gaaagcagaa atttctatgt ctacattgac ggaaaaatgc gcgactggat cattgatgag
     2881 ttcgatcagg gcaaggatct gctgaacatc aaaaagcaga tccgcgcaag gaccaatctg
     2941 agtgtcgata tggaccagat tcgggaagcc attgtgttga gctatgtgga ttccggtgaa
     3001 agcgaaaagg cttttcagct ggcgtgcgcc ttcagggatc aatcgccaac ggcgaatatt
     3061 cataacaccg ttatcaagtg catgctttcc tcgtgggagt acagtcagca gctgtttctg
     3121 gacgagagta ggaagtgggc tgcgctatat gcgaatgaaa ggatgggaaa gtttgcggga
     3181 tataaaaatg cgaaaccacg aatcggattt gtctgtgact atggatcaac ggtttttggg
     3241 gagaatgcga tatttccatt gtgtcatgcg ctgagccatt caggatttga tgtttattac
     3301 tacaattttg agcgcaatgt tttcagtata agtgacaaca gtataaacat attgaacggt
     3361 tatgatttgt cttcgggcgc gctttcggaa cggataaaaa aggacaaaat tgacattttg
     3421 gtggatctga atggacgcct gcgcgaacat caccgcttgg gggtttttgc gcaaaggtca
     3481 gcgccggtgc aggtcaacta cttcaatctg gtgggcacca tgggaatgca gaattacgat
     3541 tacatagtgg tggatgagat gcaggttccc cgttcggatg agcagtacta tactgaaaag
     3601 gcattgcacc tgccgtgtgg cgtgaatggc gcttacgctt tcaagcgcga tgtggatatc
     3661 gcagtgccag atcggggtgt gggtgttccc ttcacgttcg catccttcaa cgcattcttc
     3721 aaggtcaatg ccgttttgct ggagacctgg gctgaaattc tgcgccgtgt tcccgattcc
     3781 cgtctggtca tcaagtgcca tgaaaccaat cgcaaccgtg tcgtcaagaa aatcgccagg
     3841 gtattcagcc gggcgggcat cgatctgggc cgcatcctga tcgaagggtg gtcttcgctg
     3901 ctgcatttgc gccgccagta tgccgaagtc gatctgtgtc tggatacctt tccatacagt
     3961 ggtgggtcaa ccacgctcaa tgcgctgtgg caaggcgtac cggtattgac ctggtgtgga
     4021 gaggggtggc gtgcaaggac gtcggcttcg atgttgcaag cggccggtgt gggtgaaatg
     4081 gtggtgtcgg acaggaagga ctatgtggat gcggccgtag aatttgcgag agacttcagg
     4141 cggtccagtg atatgagaca ttttctgaaa gcgaatattg ggtcaaacgc gtattttcag
     4201 ccagagcggg tatatggtga gttggcagtt gtgttgaaag ccattgtcag gcgtgaggat
     4261 gaaacagcgc acgccatgta gggatgctca tgtcatgcct tggctggatt agagagtttt
     4321 cgacattttg caaggagagc ctgatatgtt gctgagcctg tttctgaaat atggtgtgag
     4381 tagacgggat gaaaaatctg atgaaacact ggcggattat gcggtcaggt taacatctca
     4441 tgagctttcg gctgcccgct ca
//
LOCUS       DASUEG010000142         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_880954, whole genome shotgun sequence.
ACCESSION   DASUEG010000142 DASUEG010000000
VERSION     DASUEG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880954"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..923)
                     /locus_tag="VFX11_02630"
     CDS             complement(21..923)
                     /locus_tag="VFX11_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937540.1"
                     /translation="MTTQKLQGAAPAGNVDGEMLSVISGDGVLNRLTLYRAAQDRAVL
                     LCMPAMGVRASFYRPLAVQLALAGWHVVTADLRGVGESGVRVKQGARFGYREMLEQDW
                     PANLAVVREHFPGLPVYLLGHSLGGQLNALFAAQNPGAVAGLIFSACGSVYFRGWSFP
                     ASLKILGQSQLIRGITEIVGYLPGDKVGFGGIEAKGLVRDWANVALTGRYRPAGSEFD
                     YEAALEKLQLPVLALSYTDDAFAPVSATRWLLAKMPRAQATHLTLSPSDLGASVIGHI
                     GWVKNNGRVVQQIADWLAPLTGVQ"
     gene            1124..2854
                     /locus_tag="VFX11_02635"
     CDS             1124..2854
                     /locus_tag="VFX11_02635"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX4937541.1"
                     /translation="MKATEFLLATLRETPSDAEVISHQLMLRAGMIRKLATGLYTWLP
                     TGLRVLRKVEQVVREEMNRAGAQEVLMPVVQPAELWEESGRFNAYGPELLRFKDRHDR
                     DFVLGPTHEEVITDMVRSNIQSYKQLPCNFYQIQTKFRDEIRPRFGVMRSREFIMKDA
                     YSFHMDAASLQKTYDAMYDAYTRIFTRLGLDFRAVLADTGSIGGNSSHEFHVLAQSGE
                     DDIAFSDSSNYAANIEMAEALVIGEQRPAPTQQKQQVATPGQHSIEEVAAFLKVGADQ
                     IVKTLIVHGRREKDEKEVKLVALLLRGDKELNEIKAEKNPLVASPLQFASDAEVEAVV
                     GCKPGSIGPIGLTIPVIADRSVVNMADFVCGANQDGFHLTGVNWDRDASIAEVADIRN
                     VVEGDPSPDGQGTLVIKRGIEVGHIFQLGVKYSTALKATVLDENGKNHIVPMGCYGIG
                     VTRVVAAAIEQNHDEFGIRWPDALAPFQVAIAPLNNHKSPAVQEAADHLYQELTAAGV
                     DVFYDDRKERPGVKFSDIELIGIPHRIVISDSGIEKKAYEYKHRRSGEKLEIPADSVI
                     AFLLGKLRGA"
     gene            2877..3464
                     /locus_tag="VFX11_02640"
     CDS             2877..3464
                     /locus_tag="VFX11_02640"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4937542.1"
                     /translation="MKTSSRRLFLSYLGCSLVAPAPAWAATTERPDDSMRVLLKDTIN
                     RANSFKDRFDAEVWLVDMSGRMAPFVKDPDERLHILRLAHEQARRFNLKPELVLSVMH
                     VESLFDRFAISSVGAQGLMQVMPFWRNEIGAPDDNLIDVATNIRYGCAILRAYLTRED
                     GNLMHALARYNGSRGKLWYPEKVFNAWERYWYVKA"
     gene            complement(3472..4434)
                     /locus_tag="VFX11_02645"
     CDS             complement(3472..4434)
                     /locus_tag="VFX11_02645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937543.1"
                     /translation="MSEFAPSHGLLEFIVDRINVGVFIVNQDLEVKLWNKFMAANSGV
                     NEQQVVGKKLFDCFPDLPQRWFAKKVSSVFMLKNFAFTSWEQRPYLFPFRHNRPVTGG
                     MEFMCQDLTLMPIKNDAGEVENVCIILFDVTDTAIYRSMHQAAMKKLEMVSRVDGLTQ
                     LFNRSHWQSRLAEEFSRSGRYQAPLTLIMFDLDHFKSVNDTHGHLGGDAVLVQVASLI
                     KSALRDSDIAGRYGGEEFGIILPSTPLDGAKVVAERLRASIESSPVPFEKIQIPVTAS
                     LGVAQFDPTIHDPEELIAHADAALYEAKAKGRNRVVVFEELAKA"
     gene            complement(4435..5070)
                     /locus_tag="VFX11_02650"
     CDS             complement(4435..5070)
                     /locus_tag="VFX11_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937544.1"
                     /translation="MKESLALSEEQRDCLQEVVNVAMGQAGDSLARFLEVFINLSVPR
                     IRLVARDELNRELEAMVGGPDTRVSGVTQGFYQMDNGRGIRGEAIVVFTDSSFKELAD
                     LLAYDGELGVEAEKELLLDVTNILNGACLNGIGVQMEAELGFSPPTILGQHVKISGLL
                     ENQKVNWDHALLVEISYTLEDRSFSCTMFLLMPGESIVVVKAALDRLLEEL"
     gene            complement(5067..>5336)
                     /locus_tag="VFX11_02655"
     CDS             complement(5067..>5336)
                     /locus_tag="VFX11_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937545.1"
                     /translation="ASNGIEGLEAIRAGRGEMVFLDLTMPELDGYGVLEHVKNEGHKS
                     IVIVISADIQPEARERVMKLGALDFIKKPVDGKKLQDVLKKYGLI"
BASE COUNT         1155 a         1715 c         1469 g          997 t
ORIGIN      
        1 gcccccgttt ttattggcac ttactggacg cccgtcagcg gcgccagcca gtccgcgatt
       61 tgctgcacca ccctgccatt atttttaacc cagccaatat gaccgatcac actcgcaccc
      121 agatctgacg gagacagcgt caggtgtgtg gcctgggccc tgggcatttt tgccagcagc
      181 cagcgggtgg cgctgaccgg cgcaaatgca tcgtcagtgt aggacaatgc cagcaccggc
      241 aattgcagtt tttccagtgc cgcctcgtaa tcaaattccg atcctgccgg ccgatagcgc
      301 ccggtgagcg cgacgttggc ccaatcgcgg accagcccct tcgcctcaat tccgccaaaa
      361 cccaccttgt ccccgggcag gtatccgacg atttccgtaa tgccgcggat cagctgcgac
      421 tgacccagaa ttttcagcga agccggaaat gaccagccgc gaaagtacac cgaaccacag
      481 gcagaaaaaa tcaaccccgc caccgcaccc ggattttgtg ccgcgaatag cgcattgagc
      541 tgcccgccca gggaatggcc cagcaaatac accggcagac ccggaaaatg ctcgcgcacg
      601 accgccagat ttgccggcca gtcctgctcc agcatctcgc gatagccaaa gcgggcacct
      661 tgcttgaccc gcacgccgct ctcaccgact ccgcgcaggt ccgccgtcac gacatgccag
      721 cccgccagtg ccagctgtac cgccagcggc cggtagaatg acgcccgcac ccccattgcc
      781 ggcatgcaca gcagcactgc gcgatcctgg gcagcgcgat aaagcgtgag ccggttcaat
      841 acgccgtccc cggaaatgac actgagcatt tcaccgtcga cattgcccgc cggggcggca
      901 ccctgcaact tctgtgtagt catcccatcg cactcctgac aggtttccca acccgtcttc
      961 cataaatcgc atgcctttat aaatagcatg accggcgccg ttggcccacc ctgatttcgc
     1021 cggggcgccc gccgcttgat tgtgaagcgc cacgatagcc cgtaaaatcg ggcaaatttt
     1081 ccggcatccc ctgcccacgc tctcaccgag gttgaccatt tccatgaaag ccactgagtt
     1141 cctgctagcc accctgcgcg aaacaccgtc tgacgcggaa gtcatcagtc accagttgat
     1201 gctgcgggcg ggcatgatcc gcaagctggc gactggcctt tatacctggc tgccgacagg
     1261 cctgcgggta ctgcgcaagg tggaacaggt ggtacgcgag gaaatgaacc gcgccggcgc
     1321 ccaggaagtg ctgatgccag tggtgcagcc ggcagaactg tgggaagaat ccggccgctt
     1381 caacgcctat ggtccggaac tgctgcgttt caaggaccgt catgatcgcg atttcgtgct
     1441 tggccccacc cacgaggaag tcatcaccga catggtgcgc agcaacatcc agagctacaa
     1501 gcaactgccc tgcaatttct atcagatcca gaccaaattc cgcgacgaga tccgtccgcg
     1561 cttcggcgtg atgcgttcgc gcgagttcat catgaaggac gcctactcct tccacatgga
     1621 cgcagcgtcg ctgcaaaaaa cctacgatgc catgtacgac gcctataccc gcatcttcac
     1681 ccgcctgggc ctggatttcc gcgccgtact ggctgacacc ggcagcatcg gcggcaacag
     1741 ctcccacgaa ttccacgtgc tggcgcaatc cggtgaagac gatatcgcct tcagcgacag
     1801 cagcaattac gccgcgaaca tcgaaatggc cgaagcactg gtgatcggtg agcagcgccc
     1861 tgctcccacc cagcagaaac agcaggtggc cacgccaggc cagcacagca tcgaggaagt
     1921 cgcagcgttc ctgaaagtcg gcgcagacca gatcgtgaaa accctgatcg tgcacggccg
     1981 gcgcgagaaa gacgaaaaag aagtgaaact ggtggcgctg ctgctgcgcg gtgacaagga
     2041 actgaacgag atcaaggcgg agaaaaatcc gctggtggcc tcgccgctgc aatttgccag
     2101 cgatgcggag gtggaagcgg ttgtgggctg caaacccggc tccatcggcc ccatcggcct
     2161 gaccattccg gtaatcgcag accgcagcgt ggtgaacatg gcagacttcg tctgcggcgc
     2221 caaccaggac ggcttccacc tcaccggcgt caactgggac cgtgacgcca gcattgccga
     2281 ggtggccgac atccgcaacg tagtggaagg cgatcccagt cccgatggcc agggcaccct
     2341 ggtgatcaag cgcggcatcg aagtaggcca cattttccag ctgggagtga aatacagcac
     2401 cgcactgaaa gccacagtgc tggacgaaaa cggcaagaac cacatcgtac ccatgggttg
     2461 ctacggcatc ggcgtaaccc gcgtggtggc tgccgccatc gagcagaacc acgacgaatt
     2521 cggcatccgc tggcccgacg ccctggcacc gttccaggtg gcgattgcgc cgctcaacaa
     2581 ccacaagtcc cccgccgtgc aggaagccgc cgaccacctg taccaggaac tgactgcggc
     2641 cggcgtcgac gtattctatg acgaccgcaa ggaacgcccg ggcgtgaagt tttccgatat
     2701 cgagctgatc ggcattcctc accgcattgt catcagcgac agcggcatcg agaaaaaagc
     2761 ctatgaatac aagcaccgcc gcagcgggga aaagctggaa attccggccg attccgtgat
     2821 cgcgttcctg ctgggcaagc tgcgcggcgc ctgatgccgg tctaaaatga cggagtatga
     2881 aaacgtctag ccgccgcctg ttcttgtcgt atctgggatg cagtctggtc gcccccgccc
     2941 cggcgtgggc ggccaccacc gagcgcccgg acgacagcat gcgcgtcctg ctgaaagaca
     3001 ccatcaaccg cgccaacagc ttcaaggacc gcttcgacgc cgaagtctgg ctggtggaca
     3061 tgtccgggcg catggcccct ttcgtgaaag atccggacga gcgtctgcac atcctgcgcc
     3121 tggcccacga gcaggcccgc cgcttcaacc tgaagccgga gctggtgctg tcggtaatgc
     3181 atgtggaaag cctgttcgac cgcttcgcca tctccagcgt gggagcacag gggctgatgc
     3241 aggtgatgcc gttctggcgc aatgaaatcg gtgcgccgga cgacaacctg atcgacgttg
     3301 ccaccaacat ccgctacggc tgcgccatcc tgcgcgccta cctcacccgc gaggacggca
     3361 acctgatgca tgccctcgcc cgctataacg gcagccgggg caaactctgg tatccggaaa
     3421 aggtattcaa tgcctgggaa cgttactggt acgtgaaagc ctgacgcgtt ttcaggcctt
     3481 cgccagctct tcgaacacca ctacccggtt gcgccctttt gccttcgctt catacagcgc
     3541 ggcatcggcg tgcgcgatca gttcttccgg atcgtgaatg gtgggatcaa attgcgcgac
     3601 acccagactg gcggtcacgg gaatctggat cttttcgaac ggcaccggcg aagactcgat
     3661 ggacgcgcgc aggcgctcgg cgaccacttt ggcgccatcc agcggagtac tgggcagaat
     3721 gatgccgaat tcctcgccac cgtaacggcc ggcaatatcc gaatcacgta gcgcactctt
     3781 gatcagggac gccacctgca ccagtaccgc atcgccaccc agatggccat gggtgtcatt
     3841 gacggatttg aaatggtcca ggtcaaacat gatcaacgta agcggcgcct ggtagcggcc
     3901 ggaccggctg aattcttccg ccagccgcga ctgccagtgg ctgcggttga acagctgcgt
     3961 gaggccatcc accctgctca ccatttccag ctttttcatc gccgcctggt gcatggagcg
     4021 atagatcgcg gtgtcggtca catcgaacag gatgatacag acattctcca cctcgccagc
     4081 gtcattcttg atgggcatca gcgtcagatc ctgacacatg aactccatgc caccggtaac
     4141 cgggcgattg tgacgaaacg ggaagagata cgggcgttgt tcccaggacg tgaaggcaaa
     4201 attcttcagc atgaacacgc tgctgacttt cttggcaaac cagcgctggg gcagatcggg
     4261 aaaacagtcg aacagctttt ttcctaccac ctgctgctcg ttgacgccgc tgttggcggc
     4321 catgaatttg ttccagagtt tgacttcaag atcctggttg acgatgaaca cgccaacatt
     4381 gatgcgatca acaatgaatt ccagcaagcc gtgagagggg gcgaactcgc tcactcagag
     4441 ttcttccagc aggcggtcca gcgccgcctt gacgaccaca atggattcgc ccggcatcag
     4501 cagaaacatg gtgcagctga acgagcggtc ttcgagggtg taggaaattt ccaccagcag
     4561 cgcgtgatcc cagtttactt tctggttttc cagcaggccg gaaattttga catgctgacc
     4621 cagaatcgtt ggaggggaga aacccagctc cgcctccatt tgcacgccaa tgccgttgag
     4681 acaggcgcca ttcagaatat tggtaacgtc cagcagcagt tctttttccg cttcaacacc
     4741 cagctcgccg tcgtaggcca gcagatcggc cagttccttg aacgagctgt cggtgaacac
     4801 cacgatggcc tcgccgcgaa tgccgcgacc attgtccatc tggtaaaagc cctgggtgac
     4861 accggacacc cgggtatcgg gcccgcccac catcgcttcc agttcgcgat tgagttcgtc
     4921 ccgcgccacc agccggatac ggggcacgga caaattgatg aagacttcca gaaaccgggc
     4981 cagtgaatcc ccagcctgac ccatggcgac attcaccact tcctgcaggc agtcccgctg
     5041 ctcttccgat agcgctagcg attctttcat atcagtccgt acttcttcag cacatcctgc
     5101 agctttttgc catccacagg tttcttgatg aaatccagcg cgcccagctt catgactcgc
     5161 tcgcgggctt ccggctgaat gtcggcggag atcacaatca cgatcgactt gtggccttcg
     5221 tttttcacat gttccagcac accgtaacca tccagttccg gcatggtaag gtccagaaac
     5281 accatctcac cccgcccggc gcggatggct tccaatccct cgattccgtt ggaagc
//
LOCUS       DASUEG010000143         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_732632, whole genome shotgun sequence.
ACCESSION   DASUEG010000143 DASUEG010000000
VERSION     DASUEG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732632"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFX11_02660"
     CDS             <1..355
                     /locus_tag="VFX11_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEX4937546.1"
                     /translation="LYLLQKQGEGGAQKLARALGAGLRKFAPWLMKTLSVVGTAAMFL
                     VGGGILVHGIPGSHDWMHHLEEAVAALPAAGVVLAWLTPSLVSAVVGIVAGALVLALV
                     SLVQRVIAAFRSKPA"
     gene            371..1030
                     /gene="bluB"
                     /locus_tag="VFX11_02665"
     CDS             371..1030
                     /gene="bluB"
                     /locus_tag="VFX11_02665"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009833361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEX4937547.1"
                     /translation="MKNWQFTAADQEVLQHIMQLRRDVRGNRFCRDEIPAAELDRILQ
                     AATWAPSVGFSQPWQFVLVRDRAVREKIAASFAEENRKAAPLFADKKREQYVQLKLEG
                     ILETPLNIAVFYTPSPQPVLGQTSISEVGEYSVVCAVQNMWLMARALNIGMGWVSILD
                     PSTVKNVLNAPADHKLVAYLCVGYVTEFCEQPELETLQWEPRRPLPSVVLTDSYPVPE
                     S"
     gene            1137..2414
                     /locus_tag="VFX11_02670"
     CDS             1137..2414
                     /locus_tag="VFX11_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017443936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4937548.1"
                     /translation="MKLESIALHHGYTAEATTKAAAVPIYQTTSYTFDNTQNGADLFD
                     LKVAGNIYSRIMNPTTAVLEQRLAQMEGGIGALGLASGMAAITYAIQCITRTGDNIVS
                     TSQLYGGTYNLFAHTLPQQGVTVRMASFDDFAGLEKLIDDKTRALFCESIGNPAGNVV
                     DIQKLAEIAHRHGVPLIVDNTVATPYLCRVFDHGADIAIHSLTKYIGGHGTTIGGVII
                     DSGQFDWVANKKRFPMLNEPDPSYHGVVYTDALGPAAFIGRCRVVPLRNTGAALSPHS
                     AFLILQGLETLGLRIERHCENAEKVAAYLQKHPKINWVKYAALPDSPYFETCKKITGG
                     KASGILSFGIKGGKEAGGQFIDALQMILRLVNIGDAKSLACHPATTTHRQLNPQELQK
                     AGVSEDLVRISVGIEHIDDIIADIEQALAAVSA"
     gene            2628..3125
                     /locus_tag="VFX11_02675"
     CDS             2628..3125
                     /locus_tag="VFX11_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX4937549.1"
                     /translation="MSSETESIAIFVDVQNVYYTTRQAYSRNFDYNAFWARVTANGSV
                     TNAIAYAIDKGDAKQRQFQNILKAIGFEVKLKPYIQRADGSAKGDWDVGITLDVMEWA
                     PQVQRIVLVSGDGDFDLLVQRVRDLYGVKVDVYGVPQLTAASLVRAATTFIPIDASLL
                     LEIKS"
     gene            3178..3642
                     /locus_tag="VFX11_02680"
     CDS             3178..3642
                     /locus_tag="VFX11_02680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4937550.1"
                     /translation="MSKQVSTNQQRDHYSWTYRLRVRYSEIDYQGIVFNAHYLTYFDV
                     ALTEFMREQNFDFVELVKRDQLDFHLVKSTVEYRQPIRADQLIEIGVRPGRLGQSSVT
                     WHLAIFAEGQDDCLASGEIIWVCSKVGAHKSHPLPEQFRAKVQPLLDATPST"
     gene            3658..4053
                     /locus_tag="VFX11_02685"
     CDS             3658..4053
                     /locus_tag="VFX11_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4937551.1"
                     /translation="MVLLTAYYASLMAIILVYLAVRVTLCRRRFQIGLGDGGNQELSV
                     LIRAHANALENIPFTLVLMLLAELNGLAPAYLHAAGIALLVSRLAHAYGFIVARGAYA
                     RARAFGILVNWILLLALAFANVGLVYAQY"
     gene            complement(4050..>4722)
                     /locus_tag="VFX11_02690"
     CDS             complement(4050..>4722)
                     /locus_tag="VFX11_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4937552.1"
                     /translation="FVSAVSHELKTPLTSIRMFSEMLKEGWVGDDKRREYYHFISDES
                     ERLSRLIANVLQLARMERNDLPLDVRAQPVATLLDLIRSRVTSQVERSGFQVTFDCAP
                     ACAELQVLADADALVQIMLNLVDNALKFSGKAERRQIDISIALADGGLVLGVRDYGPG
                     IPAGQIRRIFDLFYRVGSELTRETQGTGIGLALVRQLARAMGGEISVRNAQPGAEFRL
                     WLRLD"
BASE COUNT          940 a         1437 c         1401 g          944 t
ORIGIN      
        1 attgtacctg ctgcaaaagc agggcgaggg tggcgcgcag aagctggcgc gggcactggg
       61 cgcgggcctc cggaaatttg caccctggct gatgaagacc ctgtcggtgg tgggcactgc
      121 tgccatgttc ctggtgggcg gcggcatcct ggtgcacggc attcccggct cccacgactg
      181 gatgcatcat ctggaggaag ccgtggctgc gctgccggca gcaggtgtgg tgctggcctg
      241 gctcacgccc agcctggtga gcgcggtggt gggcatcgtc gccggcgcac tggtgctggc
      301 gctggtgagt ctggtgcagc gcgtcattgc cgctttccgt tccaaaccgg cctgagtatt
      361 gacgtccgcc atgaagaact ggcaattcac tgcagccgat caggaggtgc tgcagcacat
      421 catgcagctg cgccgcgacg tgcgtggcaa tcgcttctgt cgcgatgaga ttcccgccgc
      481 cgaactggac cgcattctgc aggccgccac ctgggctcca tcggtgggat tttcccagcc
      541 gtggcaattc gtgctggtgc gcgatcgcgc cgtgcgggaa aaaatagccg ccagtttcgc
      601 cgaggaaaac cgcaaggccg cgccgctctt tgccgacaaa aaacgcgaac agtatgtgca
      661 gctgaaactg gaaggcattc tggaaacgcc gctcaacatc gcggtgtttt acacgccgtc
      721 gccgcaaccg gtgctgggcc agaccagcat cagtgaagtg ggcgaataca gcgtggtgtg
      781 cgcggtgcag aacatgtggc tgatggcgcg ggcgctgaat atcggcatgg gctgggtcag
      841 catcctcgat ccatcgacgg tgaaaaacgt gctcaacgca ccggcggatc acaagctggt
      901 ggcctatctg tgtgtcggct acgtgaccga attctgcgag cagccggaac tggagaccct
      961 gcagtgggaa ccgcgccggc ctctcccgtc cgtggtgctg acagattcct atcccgtgcc
     1021 ggagtcctga tcccggttcc accggagtgt gattttttgc ggtagattat caggcagctg
     1081 ttgtttatgt tttgccaatc cgacaaccca gccgacaacc cagccgagac gccaccatga
     1141 aactggaatc catcgccctt caccacggtt acaccgccga agccaccact aaggctgccg
     1201 ccgttcccat ctaccagacc acgtcctaca ccttcgacaa cacccagaac ggcgccgacc
     1261 tgttcgacct gaaggtggcc ggcaacatct acagccgcat catgaatccc accaccgccg
     1321 tactggagca gcgcctggcg caaatggaag gcggcatcgg cgcgctggga ctggcctcgg
     1381 gcatggcggc gatcacctac gcgatccagt gcatcacccg cactggcgac aatatcgtca
     1441 gtaccagcca gctgtatggc ggcacttaca acctgttcgc gcacaccttg ccgcagcagg
     1501 gcgttaccgt gcgcatggcg tcgttcgacg atttcgccgg gctggaaaaa ctgatcgacg
     1561 acaagacccg cgcgctgttc tgcgaatcca tcggcaaccc ggcgggcaat gtggtggaca
     1621 ttcaaaagct ggcagaaatc gcgcaccggc acggcgtgcc gctgatcgtc gacaacacgg
     1681 tggccacacc ctatctgtgc cgcgtcttcg atcacggcgc cgacatcgcc atccactcgc
     1741 tgaccaaata tatcggcggc cacggtacca ccatcggcgg tgtgatcatc gattccggcc
     1801 agttcgactg ggtggcgaac aaaaaacgtt tcccgatgct gaacgaaccc gatccgtcct
     1861 atcacggcgt ggtgtacacc gacgcgctcg gccccgcggc cttcatcggc cgctgccgcg
     1921 tggtgccgct gcgcaacacg ggtgcggcgt tgtcgcctca cagcgcgttc ctgattctgc
     1981 aggggctgga aaccctgggc ctgcgcatcg aacgtcactg tgaaaacgcg gagaaagtgg
     2041 cggcctatct gcagaagcat ccgaaaatca actgggtgaa atacgctgcg ctgcccgaca
     2101 gcccttactt cgaaacctgc aagaaaatca ccggcggcaa ggcatcggga attctcagtt
     2161 tcggcatcaa gggtggcaag gaagccggcg gacagttcat cgatgcgctg cagatgattt
     2221 tgcgactggt gaacatcggc gacgccaaat ccctggcctg ccatcctgcg accaccaccc
     2281 accgccagct caatccgcag gaattgcaga aggccggcgt gtcggaggat ctggtgcgca
     2341 tttccgttgg catcgagcat atcgacgata tcattgccga tatcgagcag gcgttggcgg
     2401 cggtcagcgc gtaaagcctg ccatttgcaa tgttctttca gactggcggt atcaaatcag
     2461 cataccgcca gcgctgagcg ttgcgccgcg gtcaggtggc aagactatcg ggatgaggtg
     2521 cggtatcctg cagacaacag tcattggctg caatgacagc aggatgccag caagggaaac
     2581 aggtccatgg aaatactgaa gcacgcactt ccacccaggc ggccgcgatg agtagcgaga
     2641 ctgaatccat tgccatcttt gtcgatgtcc agaatgtgta ttacaccacc cggcaggcct
     2701 acagccgcaa tttcgattac aacgctttct gggcccgcgt cactgccaat ggcagcgtga
     2761 caaacgccat cgcctatgcc atcgacaaag gcgacgccaa acagcgccag ttccagaata
     2821 tcctcaaggc catcggtttc gaggtgaagc tgaaacccta catccagcgt gccgacggtt
     2881 cggcgaaggg cgactgggat gtgggcatca cgctggatgt gatggaatgg gcgccccaag
     2941 tgcagcgcat cgtgctggtg tccggcgacg gtgatttcga tttgctggtg cagcgtgtcc
     3001 gcgacctgta cggcgtcaag gtggatgtat acggcgtgcc gcaactgacg gcggcgtcgc
     3061 tggtgcgggc ggcaaccact ttcattccga ttgatgcctc cctgctgctg gaaataaaat
     3121 cctgatgtgc gggaccaatc caacactacc ggttatcttc atgagcagat actcttcatg
     3181 agcaaacaag tctccacgaa ccagcagcgc gatcactatt cctggactta ccgcctgcgg
     3241 gtgcgttact cggaaatcga ttaccagggc attgtgttca acgcccacta cctgacctat
     3301 ttcgatgtgg cgctgacgga gttcatgcgc gaacagaatt tcgattttgt cgagctggta
     3361 aaacgcgatc agctggattt ccatctggtg aaatccacgg tggaataccg gcagccgatc
     3421 cgggctgacc agttaatcga aattggcgtg cggcccggtc gtctgggtca gtccagtgtc
     3481 acctggcatc ttgccatctt tgcagaaggt caggacgatt gcctggccag cggcgaaatc
     3541 atctgggtgt gttcgaaagt gggtgcacac aaatcgcatc cgctgccgga acagtttcgc
     3601 gccaaagtgc agccgctgct ggatgccaca ccttcaacct gatcggggac agcgctcatg
     3661 gttctgctga cggcctatta tgcttcgctc atggccatca ttctggtcta tctggctgtg
     3721 cgtgttacct tgtgccgccg gcgtttccag attggtctgg gtgacggcgg caatcaggag
     3781 ttgagtgtgc tcatccgtgc ccacgccaat gccctggaaa atattccctt cacgctggtg
     3841 ttgatgctgc tggctgaact gaatggcctg gcgccggcgt atctgcatgc cgccggcatt
     3901 gcactgctgg tgtcgcggct ggcccacgct tatggcttca tcgtggcccg cggtgcttac
     3961 gcccgcgctc gcgcctttgg aattctcgtc aactggatac tgctgctggc actggcgttt
     4021 gcgaacgtgg ggctggtgta tgcgcagtat taatccagcc gcaaccataa cctgaattcc
     4081 gcgcccggtt gcgcattgcg cacactgatc tcgccaccca tggcccgcgc cagttgccgc
     4141 accagcgcca gaccgatgcc ggtgccctgg gtttcccgcg tcagctcgct gccgacgcgg
     4201 taaaacaaat cgaaaatccg tcgaatctga cccgctggaa tgccggggcc gtaatcgcgc
     4261 acgcccagta ccagaccgcc atccgccagt gcgatggata tgtcgatctg ccggcgttca
     4321 gccttgccgg aaaatttcag tgcgttgtcc accagattca gcatgatctg caccagcgcg
     4381 tcggcatcgg ccagcacctg caattcggcg caggccggcg cgcaatcgaa cgtcacctga
     4441 aatccgctgc gctccacctg tgaggtcacg cgggagcgga tcagatccag cagcgttgcc
     4501 accggctgcg cgcggacatc cagcggcaga tcattgcgtt ccatccgcgc cagttgcagc
     4561 acgttggcga tcagccgcga cagccgttcg ctttcgtcgc tgatgaagtg gtagtactcg
     4621 cggcgcttgt cgtcgccgac ccagccttcc ttcagcatct cgctgaacat gcggatggag
     4681 gtgagcggcg ttttcagttc atggctgacg gcggacacaa aa
//
LOCUS       DASUEG010000144         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_957017, whole genome shotgun sequence.
ACCESSION   DASUEG010000144 DASUEG010000000
VERSION     DASUEG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957017"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFX11_02695"
     CDS             <1..789
                     /locus_tag="VFX11_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937553.1"
                     /translation="PVVIFLHGFAALYPALYRTHIDHLVQQGTIVIFPQFQKSTLSGF
                     LREAGLGQAADQNVWAQRAVDSVAQVLTQLGDQVQQDEIYLYGHSLGGLIALAWQANG
                     GVPVNAIVLSHPQVNAQEGIPPFVRAFLKIIEIPWRDYAPSISAPVIILNGNEDTIAP
                     VSQSEEILSLLPGVPSKVLYVAQRDTYGYPKISPNHGAPLDDIKGLPDHLKFFNISGE
                     LDALDYRYYFAGLDAVMDGLRDELPFDLGTWSNGRPVKPVQQFH"
     gene            880..1407
                     /locus_tag="VFX11_02700"
     CDS             880..1407
                     /locus_tag="VFX11_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014753380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937554.1"
                     /translation="MCRLPVLSAFLSLLIAFTTACSSAPPVKPLTIGSQVDFSALEDQ
                     NGNAFTHEDVMQTLLFVDTMKAKNLVRDELATIDLSCLDAGRVVYLADISGMPSLISK
                     LIAVPRMRDYDYPIWLDYSGLATDPLPTHENEVTYIQLDGTAIRTIEFIADAKVLHTR
                     LVAQCGPAKQQMATY"
     gene            1421..2338
                     /locus_tag="VFX11_02705"
     CDS             1421..2338
                     /locus_tag="VFX11_02705"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937555.1"
                     /translation="MTAKFRGLAAALTILLLLLAGCTGTRLLVANGIARSGDYRLSAD
                     LAYGDDPRQRLDVYLPEQHLPARPTLIFVPGGCWGACETYPKEDYRFVADAFTAQGYA
                     VVIPDYRLYPAVKFATIIGDVRDAVEWVVHHGAQHGLNTDALVLMGHSAGAQMTALLT
                     LDESYLSADTRTRLKGYVGLAGPYDFLPFTKSYQPDLFGPESLYPASQPVNFVGGDEP
                     PLLLLYGTADTTVKPRNILSLTRRVQEQGGAVQAHCYAGIDHGGIVGALSRPLQDDTP
                     VFADILAFLAQIGRPAAVAHASPLQCSAS"
BASE COUNT          426 a          728 c          731 g          492 t
ORIGIN      
        1 cccgttgtca tttttctgca cggatttgca gcgctttacc ccgcgctgta ccgcacccac
       61 atcgaccatc tggtgcagca gggcactatc gtgatcttcc cgcaattcca gaaaagcacc
      121 ctgagcggct tcctgcgtga agcggggctg ggtcaggcgg cggaccagaa tgtctgggct
      181 cagcgggcag tggactcggt ggcgcaggtg ctgacgcagc tgggcgatca ggtgcagcag
      241 gatgaaatct atctgtacgg ccattccctg ggcggtctca tcgcgctcgc gtggcaggcc
      301 aatggcggtg tgccggtcaa tgccattgtg ctcagccatc cgcaggtgaa cgcgcaggaa
      361 ggcattccgc ctttcgtacg ggcctttctg aaaatcatcg agattccctg gcgggactat
      421 gcgccgtcca tcagcgcccc ggtgatcatc ctcaacggca acgaggacac tatcgccccg
      481 gtgagccaga gcgaggagat tctgtcgctg ctgccgggcg tgccgtcgaa agtgctgtat
      541 gtggcgcagc gggatacgta tggctacccg aaaatttccc ccaaccacgg cgcgccactg
      601 gacgatatca agggcctgcc ggaccacctg aaattcttca atatctcggg tgagctggat
      661 gcactggatt accgctacta ttttgccggc ctggatgcgg tgatggatgg cctgcgcgat
      721 gagctgccat ttgatctggg cacctggagc aacggacgcc cggtgaagcc ggtacagcag
      781 tttcattgac cggtatcgtc aggggagtgg aatcaggctt gcgccggtcg cacgcctgac
      841 tctaccatcc ggatattcgt tcccttccga gatacgccca tgtgccgcct gcctgttctg
      901 tccgctttcc tgagccttct gattgccttc accactgcct gttcctccgc tccgccggtg
      961 aagccgctca ccatcggcag ccaggtggat ttttccgcgc tggaggatca gaacggcaat
     1021 gcattcacac acgaggacgt gatgcagaca ttgctgttcg tggatacgat gaaggccaag
     1081 aatctggtgc gcgatgaact ggcgaccatc gacctgtcct gcctggatgc tggacgggtg
     1141 gtgtatctgg cggatatcag tggcatgccc agcctgattt ccaagctgat cgcggtaccg
     1201 cgcatgcgtg actacgatta tccgatctgg ctggattaca gcggtctggc cactgatccg
     1261 ctgccaaccc atgaaaatga agtgacctac atccagctgg atggcactgc gatccgcacc
     1321 atcgaattca tcgcggatgc caaggttctg cacaccagac tggtggcgca atgcggcccg
     1381 gccaagcagc agatggcgac ctactgaaca cccgcccgtc atgactgcga aattccgggg
     1441 actggccgct gcactgacaa tcctgttgct gctgttggct ggttgcaccg gtacccgtct
     1501 gctggtggcc aacggcattg cccggagcgg cgattaccgg ctgagtgccg acctggccta
     1561 tggcgatgat ccccgccagc ggctggatgt ctatttaccc gaacagcatt taccggcacg
     1621 ccccaccctc atttttgtac ccggcggttg ctggggcgcc tgcgaaacct atcccaagga
     1681 ggattaccgc ttcgtggccg acgcgtttac ggcacaaggc tacgccgtgg tgattccgga
     1741 ttaccggctg tacccggcgg tgaaattcgc caccatcatc ggtgacgtgc gggatgcggt
     1801 ggagtgggtg gtgcatcatg gagcgcagca cgggctgaac acggacgcgc tggtgctgat
     1861 ggggcattcc gctggcgcac agatgacggc gctgctgacg ctggacgagt cgtacctgtc
     1921 agcagacacc cgcacccgtc tgaaaggcta tgtcgggctg gcggggccct acgatttcct
     1981 gcccttcaca aaatcctacc agccggacct gttcgggccc gagtcgctgt atcccgcatc
     2041 gcagccggtg aattttgtcg gcggcgatga accgcccctg ttgctgctgt atggcacagc
     2101 ggacaccacg gtgaaaccgc gcaatatcct gagcctgacc cggcgtgtgc aggagcaggg
     2161 cggggcagtg caggcgcact gctacgcggg tatcgatcat ggtggcatcg tcggcgccct
     2221 gtcgcggccg ctgcaggacg acacgccggt gtttgcggac atcctggcct ttctggcaca
     2281 gatcggtcgg ccggcggcgg ttgcccacgc ctcgcccctg caatgcagcg cttcctgaac
     2341 cctgcagtct gatcctggcc gtggggttat ctggccc
//
LOCUS       DASUEG010000145         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_559372, whole genome shotgun sequence.
ACCESSION   DASUEG010000145 DASUEG010000000
VERSION     DASUEG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559372"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFX11_02710"
     CDS             complement(<1..204)
                     /locus_tag="VFX11_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937556.1"
                     /translation="MSKPTWQDPHAGREAERYENPIPSRELILELLGKGPKALTADQV
                     AAHFGLFEEDRAEALRRRLGAMIR"
     gene            311..395
                     /locus_tag="VFX11_02715"
     tRNA            311..395
                     /locus_tag="VFX11_02715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Leu,seq:cag)
     gene            complement(529..1701)
                     /locus_tag="VFX11_02720"
     CDS             complement(529..1701)
                     /locus_tag="VFX11_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937557.1"
                     /translation="MSVTVPPSVAPMIPRTLFNSDHEAFRATARRFFETEIAPFHEKW
                     EEQQHIDRNLWNKAGELGLLCCTMPEEYGGSGVDRLYSMILMEEQARVGDSASGFSLH
                     SDIVANYILNFGNEEQKKTWLPKMATGEAVGAIAMTEPGTGSDLQSIRTTAVADGDDY
                     IVNGSKIFITNGYLCDVAVVAVKTGDIGGGAQSVSLLIMDAKMPGFTKGKPLKKVGMR
                     GQDTCELFFDNVRVPKSNLLGMEGMGFIMLMKELAWERMMIAIMCSAAAEFALATTVD
                     YVKQRKVFGKPVSAYQNTRFKLAEMRSEIQVARVFVDRCMEQVLKGELSVEAACAAKY
                     WVSDLLSKVVDEGVQLHGGYGFMLEYPIARAYIDNRANRIYGGTNEIMKELISRSI"
     gene            1915..2622
                     /locus_tag="VFX11_02725"
     CDS             1915..2622
                     /locus_tag="VFX11_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937558.1"
                     /translation="MSPPSSAPDTTTDGTTASRRTQLERSEAMRQRLIDATLSCLESE
                     GYAGTTISKIVVRAEVSRGAPVHHFPTKAALIEATAEQLVRHVYVRLGEVLLALNDSE
                     DRLRAVVVNAWQTVFGTREHAAMMELLLASRHDEELAVMMKRLWTSGYLLIQGAAEHY
                     FEPVDSQGTPLDMMILMQWLLRGMALDRHLVDDTRFYDHFLDLWCGLLATRIRPRQGV
                     TTPPPRPGFWDETLARK"
     gene            complement(2700..3122)
                     /locus_tag="VFX11_02730"
     CDS             complement(2700..3122)
                     /locus_tag="VFX11_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4937559.1"
                     /translation="MSQQRIEIVQEFPFPVSKLFAYLSVHENLETIFAPTKIHTVRKG
                     DDAPCGVGSVREMRIFPLPSFDETVTAYKPDELIEYRITRGSPLKNHHGVMRFYPLGE
                     GSRLHYTIVFEGKLPVVASIVKLGLERSIRQGLKGLRL"
     gene            complement(3180..3626)
                     /locus_tag="VFX11_02735"
     CDS             complement(3180..3626)
                     /locus_tag="VFX11_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX4937560.1"
                     /translation="METFAQNPALWWLSAGVFMLVLEVVGNTLFFLWFSGAMFVTALV
                     TWLFGPPPAVQGLVFAIATVFALLGWQRFRPLQKMEGRDAASGLNNRLSGFVGRELVL
                     TEAVVNGQGRVHIDDTYWVALGEDMPAGTRVRVVGLDGMRLRLERI"
     gene            complement(3630..4544)
                     /locus_tag="VFX11_02740"
     CDS             complement(3630..4544)
                     /locus_tag="VFX11_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4937561.1"
                     /translation="MGTFVLVLMVLAVVLVIMTVKQVPQGYEWTVERFGRYTRTLSPG
                     LDFIWPVFEKIGNKVNMMEQVLDIPPQQVISRDNAMVTIDAVCFYQVFSASQASYEVS
                     NLDYAIRNLTMTNIRTVIGSLDLDQMLSQRDQINSQLLSVVDQATNPWGIKITRIEIK
                     DITPPSDLVDAMASQMKAERTKRAQILEAEGTRQAAILAAEGKKQAQILEAEGERQAA
                     FLEAEARERAAEAEAKATMMVSQAIAKGDIQAVNYFVAQKYVQALGEIASSNNTKLIM
                     MPLESSQVIGSIAGIGDIVKEVQNKLKD"
BASE COUNT          940 a         1419 c         1362 g          914 t
ORIGIN      
        1 gcggatcatg gcgccgagac ggcggcgcag ggcctcggcc cggtcttctt caaacaaccc
       61 gaaatgggcg gccacctggt cggcggtcag cgccttgggg cctttgccca gaagctcaag
      121 gatgagctcc cggctgggaa tggggttttc gtaacgctcg gcttcgcggc cggcatgggg
      181 gtcctgccag gtaggcttgg acatggagac ttcctgtatg ggtggccact ggccacgaaa
      241 tgatcaaaca aacacattgt agcgcgaagg cgttgacaat cccattaagg agtaataaat
      301 tgcgcgcctc gcccaggtgg tgaaattggt agacacgcta gtttcaggta ctagtgctgc
      361 aaggcgtgga ggttcaagtc ctctcctggg caccaattca agcaaaaagg ccggtcagaa
      421 atgaccggcc tttttgtttt ttccggccgg cagccacctt cctgccaccg gcccgccaat
      481 gacaaagggc agcattgctg ctgccctttg tctccccaaa ccgccggctc agatggaacg
      541 cgagatcagt tccttcatga tttcattggt gccgccgtaa atgcggttgg cgcggttgtc
      601 gatgtaggca cgggcaatcg ggtactccag catgaagccg tagccaccgt gcagctgcac
      661 gccctcgtcc accaccttgg acagcaggtc ggacacccag tacttggcgg cgcaggcagc
      721 ttcgacactc agctcaccct tcagcacctg ctccatgcag cggtccacga agacacgggc
      781 cacctggatt tccgaacgca tttccgccag cttgaagcgg gtgttctggt aagccgatac
      841 cggcttgccg aagaccttgc gctgcttgac gtaatcaacg gtggtggcca gcgcgaattc
      901 ggctgcggcc gagcacatga tggcgatcat catgcgctcc caggccagct ccttcatcaa
      961 catgatgaag cccatgcctt ccatgcccag caggttgctc ttgggcacgc gcacgttgtc
     1021 gaagaagagc tcacaggtgt cctggccacg catgccgacc ttcttcagcg gcttgccctt
     1081 ggtgaagccg ggcatcttgg catccatgat cagcagggat acggactgtg cgccaccgcc
     1141 gatatcaccg gtcttcactg cgacgacagc cacatcgcag aggtaaccgt tggtgataaa
     1201 gatcttggag ccgttaacga tgtagtcatc accgtcggcc acagccgtgg tgcggatgga
     1261 ctgcaggtca gagccggtgc ccggttcggt catggcgatt gcacccacgg cctcgccggt
     1321 agccatcttc ggcagccagg tcttcttctg ctcttcgtta ccaaagttga ggatgtagtt
     1381 ggcgacgatg tcggagtgca gggaaaagcc ggaggccgag tcaccgacac gggcctgttc
     1441 ttccatgagg atcatggaat agaggcgatc cactcccgat ccaccgtact cctcgggcat
     1501 ggtgcagcac agcaggccca gctcaccggc cttgttccag aggttgcggt cgatgtgctg
     1561 ctgttcttcc cacttctcgt ggaagggggc gatttcggtc tcgaagaagc ggcgtgccgt
     1621 ggcacggaaa gcctcgtggt ccgagttgaa cagggtacgg gggatcatcg gggcaacgga
     1681 cggcggcacg gtgacgctca tgggagactc cttcaatgca gggtggggaa ttgactgtat
     1741 ttacaatacg gtcgtccaac atgtatcttg ataccaatcg gtcaccttgc tgtcaatcat
     1801 caggggccgt ctcttgggga cagggcgtct ggcccggcct gaccggccag ccactcatcc
     1861 ccttgcaagg tgttgatgga aaaagaaaaa ttcgccactc agcccggaga ccccatgagc
     1921 cctccttcat ccgcccccga caccacgacc gacggcacga ccgccagccg ccgtacccag
     1981 ctggagcgca gcgaggccat gcgccagcgt ctgattgacg ccaccttgtc ctgcctggaa
     2041 agcgaagggt atgccggcac caccatcagc aagatcgtgg tccgggccga ggtgtcacgc
     2101 ggagcgcccg tccatcactt ccccaccaag gcggcactga tcgaggcgac tgccgaacag
     2161 ctggtgcgcc atgtctatgt acggctgggt gaggtgctgc tggcactgaa tgattcggag
     2221 gatcgcctgc gggccgtggt ggtgaatgcc tggcagacgg tattcggcac ccgcgagcat
     2281 gcggccatga tggagttgct gctggccagt cgtcacgacg aggaactggc agtgatgatg
     2341 aagcggctgt ggacgtcggg ctacctgctg attcaggggg ccgccgaaca ctatttcgag
     2401 cccgtggact cgcagggcac accgctggac atgatgatcc tgatgcagtg gctgttgcgc
     2461 ggcatggcgc tggaccggca cctggtggac gacacgcgct tttatgacca ctttctggac
     2521 ctgtggtgcg ggctgctggc cacgcgcatc cgtccgcgcc agggcgtcac gacgccacca
     2581 ccccgccccg gtttctggga cgaaacactg gcgcgcaaat aagcctgccc gccaacgaaa
     2641 aagccggtcg ttgtgaccgg ctttttcatc acacatcacg acatcacaac atcacaacat
     2701 cacaagcgca gtcctttcag gccctggcgg atggagcgct ccaggcccag ctttacgatg
     2761 ctggccacga cgggcagctt gccttcgaac acgatggtgt aatgcaggcg cgagccctcg
     2821 cccagcggat agaaacgcat gacgccatga tggttcttga gcgggctgcc acgcgtaatg
     2881 cggtactcga tcagctcgtc cggcttgtag gccgtcacag tctcatcaaa tgacggcagc
     2941 ggaaaaatac gcatttcacg cacagagccc acgccacacg gcgcatcgtc gcctttgcga
     3001 acggtgtgga ttttggtcgg cgcaaaaatg gtttccaggt tttcgtgcac gctcaggtag
     3061 gcaaacagct tgctcaccgg aaacggaaac tcctgcacga tctcgattcg ttgctgactc
     3121 acggtgacat catcccggtt ggtggaaaaa agccccgcca ggcggggctg cagactgggt
     3181 cagatgcgct ccagacgaag ccgcatgcca tccagaccca cgacacgaac gcgcgtgccg
     3241 gccggcatgt cttcacccag cgccacccag taggtgtcgt cgatatggac acgcccctgc
     3301 ccgttcacca cggcctcggt cagcaccagc tcacgaccga caaaaccgct caggcgattg
     3361 ttgaggccgg aggccgcatc gcggccctcc atcttctgca aggggcggaa acgttgccag
     3421 cccagcaagg caaagaccgt ggcaatggca aacaccagcc cttgcacggc aggaggcggg
     3481 ccaaacagcc acgtcaccag tgccgtcacg aacatggcgc cgctgaacca gaggaagaac
     3541 agggtgttgc ccacgacctc cagcaccagc atgaagacgc cggcactcag ccaccacaag
     3601 gcaggattct gggcaaaggt ttccatggct cagtccttca gtttgttctg cacttctttc
     3661 acgatatcac cgataccggc gatggacccg atcacctgcg acgactccag cggcatcatg
     3721 atgagcttgg tgttgttgct gctggcaatt tcgcccagtg cctgcacgta cttctgggcc
     3781 acgaagtagt taacagcctg gatgtcgccc ttggcgatgg cctgcgacac catcatggtg
     3841 gccttggctt cggcctcggc ggcacgttca cgggcttcag cttccaggaa ggccgcctga
     3901 cgctcacctt cggcctccag aatctgcgcc tgcttcttgc cttcagcagc gagaatggcg
     3961 gcctgacggg taccttcggc ttcgaggatc tgcgcacgct tggtacgttc cgccttcatc
     4021 tgcgaggcca tggcatcaac cagatcggac ggcggtgtga tgtccttgat ttcgatacgg
     4081 gtgatcttga taccccaggg attggtggct tggtccacca ccgagagcag ctgcgaattg
     4141 atctggtcac gctgtgacag catctggtcc agatcgagcg aaccgatcac ggtacggatg
     4201 ttggtcatgg tcaggttgcg gatggcatag tcgaggttgc tcacctcgta cgaagcctgc
     4261 gaggcactga acacctggta aaagcagacg gcgtcgatgg tgaccatggc gttgtcgcgc
     4321 gagattacct gctgcggcgg aatatccagc acctgctcca tcatgttgac cttgttgccg
     4381 atcttctcga acaccggcca gatgaagtcc agacccggcg acagggtgcg cgtataacga
     4441 ccaaagcgtt ccaccgtcca ctcataaccc tgcggtacct gcttcacggt catgatgacc
     4501 agaaccaccg cgagcaccat cagcaccaac acaaacgttc ccattcctaa agctcctttt
     4561 gcgttttccg catttgatta aaacagttca gttgagcggc gccacgcgca gcacttcttc
     4621 aatcgtggtc tggcc
//
LOCUS       DASUEG010000146         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_408939, whole genome shotgun sequence.
ACCESSION   DASUEG010000146 DASUEG010000000
VERSION     DASUEG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408939"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFX11_02745"
     CDS             <1..257
                     /locus_tag="VFX11_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4937562.1"
                     /translation="AALDGSGHFPPMMMQMIASGESSGELDSMLSRAATNQERELEDL
                     IDTIVALFEPLMLVVMGGVVMMIVLSIMMPILSMNSLVGK"
     gene            313..750
                     /gene="gspG"
                     /locus_tag="VFX11_02750"
     CDS             313..750
                     /gene="gspG"
                     /locus_tag="VFX11_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX4937563.1"
                     /translation="MKGFKHSQALRQQGFTLIEVMIVVAILGILATLVVVSIGGRQDQ
                     ALVTATKSDLQAISQALDLYKLDNFKYPTTDQGLEALVVKPDNARNWPEGGYLKKVPV
                     DQWKNPYVYISPGSTGAYDLYSLGADGAEGGEETAADISVADL"
     gene            756..1409
                     /gene="gspH"
                     /locus_tag="VFX11_02755"
     CDS             756..1409
                     /gene="gspH"
                     /locus_tag="VFX11_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HEX4937564.1"
                     /translation="MRIHSPRHQGFTLIEIMVVVTIIGILSAMIVVNAVTSDPQKDLE
                     REARRLKAVIELAEEEALFGQVDIGIIVKENSYAFARYELPPDNAAATQPPATTPTGA
                     NAQSNVSPQWTMIEDETEFRQYELSEDYEIILEVEDDEIDPSGLSNSNNSTGPLTRPS
                     SKITEDEDAIEPAIYISPGGEITPFVMEIYIKENSDLMVKISGDEFGRIWIGDEEEI"
     gene            1393..1764
                     /gene="gspI"
                     /locus_tag="VFX11_02760"
     CDS             1393..1764
                     /gene="gspI"
                     /locus_tag="VFX11_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX4937565.1"
                     /translation="MKKKSDGFTLIEVMIALAIFAVAMGALMSTVRNNLNTVTGLQNR
                     TMAQWIASNQLVRYHAINAQMPVADRKDKLTYAGREWAIRTVVIKKTDSINEVNISVG
                     EKVGREENYYTTLTGYLSNVD"
     gene            1754..2470
                     /gene="gspJ"
                     /locus_tag="VFX11_02765"
     CDS             1754..2470
                     /gene="gspJ"
                     /locus_tag="VFX11_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX4937566.1"
                     /translation="MWTSARRERQSGITLIELMIAMAIFAVMAASMFIAFNSIQQSKA
                     GGDAASERLRQYQFMFNRMGQDFQQITPRPIRDEYGDPKGALIAGPEGGIEFTRTGWT
                     RSRFSRSQRSNLQRIQYYLEDGQLVRAYWYHLDREPAAQPTRSVLLEGVTELKFKFYY
                     SYTSDAMEPEPVEEWPPAAVLAGGGGGATAGAAPASPIIPQVEHMMLPRVIEVILTTE
                     DLGEITRLFLVADRWSTLPL"
     gene            2541..>3452
                     /gene="gspK"
                     /locus_tag="VFX11_02770"
     CDS             2541..>3452
                     /gene="gspK"
                     /locus_tag="VFX11_02770"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX4937567.1"
                     /translation="MLVTVLLMVAVAAIIATDIAYRQRLDIKRTSAFLAREQAFQVLL
                     ASEELGHAFLMEDLKADARNVSANTDPFDNLGDKWYQVVPPAPVLGGAGFVSGELEDL
                     QGRFNINSLFATDPAVAASQKQRLAQLFTNLQVPDPNAPSSSGVSASGLVDRIMDWVD
                     DNDQPTGIDGREDDAYLNQPKKPYRTANHIITDVSELLLIEEFTQADIDKIRDFICFL
                     PRDATLNLNTATEQVLRAYGFTSPDAFIKDRPARGREGTDYSDITQIMSILDPGGTVS
                     QQQAPQQPQTSSEPGDAPPPVPPAAPGS"
BASE COUNT          788 a          947 c         1015 g          702 t
ORIGIN      
        1 tcgctgccct tgatggttcc ggtcattttc cgccgatgat gatgcagatg atcgccagcg
       61 gtgaaagcag tggcgaactt gattccatgc tctcgcgcgc tgccaccaac caggagcgcg
      121 aactggaaga cctgatcgat acgattgttg ccctgttcga acccctgatg ctggtggtga
      181 tgggcggggt ggtgatgatg atcgtactgt cgatcatgat gccgatcctg agcatgaatt
      241 ccctggtcgg aaaatagggt tggaaagtaa cgtcagattc attcattaca taaaaaagga
      301 agtcgaggca ctatgaaagg tttcaagcat tcccaagcgc tgcgccagca gggtttcacg
      361 ctgatcgaag tgatgatcgt ggtggccatt ctgggtattc tcgccaccct ggttgtggtc
      421 agcatcggcg gccggcagga tcaggcgttg gtcaccgcga caaaaagcga cctgcaggct
      481 atttcccagg cgctggacct gtacaagctg gacaacttca agtatcccac gacggatcag
      541 ggcctcgaag cgctggtggt caagccggac aacgcgcgca actggccgga aggtggctat
      601 ctgaaaaagg tgccggtgga ccagtggaaa aatccgtatg tttatatcag ccccggcagc
      661 accggtgcct atgatttgta ttcgctcggt gccgatggtg ccgaaggtgg cgaagaaacg
      721 gcggctgaca tttccgtagc cgatctgtaa tttccatgcg gatccactct ccccgccacc
      781 agggtttcac actgatcgaa atcatggtgg tggtgaccat catcggcatt ctgtctgcaa
      841 tgatcgttgt gaatgccgta acgtccgatc cgcagaaaga tctggagcgt gaggcccgcc
      901 gtctcaaggc tgtcatagag ctggcggagg aggaggccct gttcggccag gtggatatag
      961 gcatcattgt gaaggaaaac agctacgctt ttgcgcgcta tgagttgcca cccgacaatg
     1021 ctgctgcaac acaaccacct gccaccaccc caaccggtgc caacgctcag tccaacgtgt
     1081 cgccgcaatg gaccatgatc gaggacgaaa ccgagttccg ccagtatgaa ctgtctgagg
     1141 actacgagat cattctggag gtggaagacg acgaaatcga tccatcgggt ctcagcaaca
     1201 gtaacaacag taccggcccc ctcacgcggc cgtcatccaa gatcacagaa gatgaagacg
     1261 caattgagcc ggcgatctac atcagtccgg gcggtgaaat caccccgttc gtgatggaaa
     1321 tctatatcaa ggaaaacagt gatctgatgg tgaaaatctc cggtgatgag ttcggccgca
     1381 tttggattgg cgatgaagaa gaaatctgat gggttcacct tgatcgaagt catgatagcc
     1441 ctggcgattt tcgccgtggc gatgggtgcc ctgatgtcca ccgtgcgtaa caacctcaat
     1501 acggtgaccg gtctgcagaa tcgcaccatg gcacagtgga ttgccagcaa ccagttggtg
     1561 cgctatcacg ccatcaacgc tcagatgccc gttgccgatc gcaaggacaa gctgacctat
     1621 gccggacgtg agtgggcgat ccggaccgtt gtgatcaaga agaccgatag catcaatgag
     1681 gtgaacatca gcgtcgggga aaaagtgggc agggaagaaa attactacac cacgctgacg
     1741 ggataccttt ccaatgtgga ctagtgcgcg gcgggagcgg cagtccggca tcaccctgat
     1801 cgaattgatg attgccatgg cgatattcgc tgtcatggcc gccagcatgt tcattgcctt
     1861 taacagtatc cagcagtcga aggccggtgg tgatgctgcg tccgaacggt tgcgccagta
     1921 ccagttcatg ttcaaccgca tgggtcagga ttttcagcag attacgccgc gcccgatccg
     1981 cgatgagtat ggtgatccga aaggcgcatt gatcgcaggc cccgaaggtg gcatcgagtt
     2041 cacgcgcact ggctggaccc gttcccgttt cagccgaagc cagcgttcca acctgcagcg
     2101 aatccagtac tacttggaag atggtcagct ggtacgcgcc tactggtatc acctggaccg
     2161 ggagccagcg gcacaaccga cgcgctcggt gctgctggaa ggggtcacgg aactgaaatt
     2221 caagttctac tacagctaca ccagtgatgc gatggagccc gaacccgtgg aggaatggcc
     2281 accggcggcc gttctggcag gaggcggtgg cggggcgacc gctggtgcag cgccggcttc
     2341 acccatcatc ccgcaggtgg aacacatgat gttgccccgg gtgatcgaag tgattttgac
     2401 caccgaagat ctgggtgaaa ttacccgtct gttcctggtg gctgaccgct ggagcacatt
     2461 accgctatga agaaatggtc gctatgaaaa gccggtgtgc tgcaaagatt ccgaccgtat
     2521 cggcgcgctc aaggggcact gtgctggtga cggtgctgct gatggtcgcc gtggccgcca
     2581 tcatcgcaac cgatattgcc taccgccagc ggctggatat caaacgtacc agcgcttttc
     2641 tggcgcggga gcaggccttc caggttctgc tggcgtcgga agaactgggc catgcttttt
     2701 tgatggaaga tctgaaggcc gatgcccgca atgtcagcgc caacaccgat cccttcgaca
     2761 atctgggaga caagtggtat caggtagtgc cacccgcgcc cgtgctcggt ggtgccgggt
     2821 ttgtcagtgg tgaactggaa gacctgcagg gacggttcaa catcaactcg ttgtttgcga
     2881 cagatccggc cgtggctgca tcacaaaaac agcgcctggc gcagctgttc accaatttgc
     2941 aggtacctga tcccaatgcg ccgtccagtt ccggcgtttc ggcatccggt ctggtggacc
     3001 gcataatgga ctgggtggat gacaacgacc agccgaccgg tatcgacggg agggaggatg
     3061 atgcctacct caaccagccc aaaaagccct atcgaaccgc caatcacatc atcacggacg
     3121 tttcggagct gctgctgatc gaggagttca cgcaggcgga tatcgacaag atccgcgatt
     3181 tcatctgctt cctgccaagg gatgccacgt tgaatctcaa tacagccact gagcaagtgt
     3241 tgcgggccta cggattcacg tcaccggacg ctttcatcaa ggacaggccg gcccggggca
     3301 gggaagggac ggattattcg gacataacac agatcatgtc gattctggat cccggcggta
     3361 ctgtgtccca gcagcaagcc ccgcagcaac cgcagacatc ctcggaacca ggagatgcac
     3421 ccccccctgt gccgccagca gcccccggaa gc
//
LOCUS       DASUEG010000147         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_200111, whole genome shotgun sequence.
ACCESSION   DASUEG010000147 DASUEG010000000
VERSION     DASUEG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200111"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFX11_02775"
     CDS             complement(<1..198)
                     /locus_tag="VFX11_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937568.1"
                     /translation="MPSVQQYQHKPSVFIDFRFFQGNPANTTNPVEMAAAKTQLQPTI
                     ERTLQESQLFSRYTFDEFEKEK"
     gene            complement(611..1942)
                     /locus_tag="VFX11_02780"
     CDS             complement(611..1942)
                     /locus_tag="VFX11_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4937569.1"
                     /translation="MRSFTRLSHATRLGLNVERFFQRMRARHGNTFTVLMPGLNDVVF
                     TSEPALVSQLLQADSDTLSSSLPSPLQPLLGNGSLILLQGRAHTQERQQLRPLFQGAC
                     LHRYSGFIRQAISDACHPLSSPVDAQAFMKMVTLDVILRAVFGIDTPDRRALFSAAIE
                     RLLNAFTSLLLLVPQARFSLFGISPWDRFLQARQTLDDLIFAEIRQRAPGREADDILS
                     HLLRQHASQSPLTEADLPHIRDQLTTLLLAGHETTANSLAWALYYLATHQQAQTSIRQ
                     QLGEQAAAPQETQNSAAMKSERLDAFCKEVLRLRPVVPLVIRSVLQPFTLGQHPLRPG
                     TYVGVATHNLHTDPDLYSHPFQFDPERFLRRKYKANEFVPFGGGDKKCLGYGFALHEM
                     KLILAHLLERYTIHLHQAVLPESSIQGLTMGPRQRILISLKAPQQATGIRS"
BASE COUNT          391 a          509 c          693 g          444 t
ORIGIN      
        1 tttttctttt tcgaattcat caaatgtgta gcgactgaag agctggcttt cctgcagcgt
       61 gcgctctatg gtcggctgga gctgggtttt ggcagccgcc atttccaccg gattggtcgt
      121 gtttgccgga ttgccctgga agaagcggaa atcaatgaac acggaaggct tgtgctgata
      181 ttgctgaacg ctgggcattg cggttacttc aggaacctgg tttttggcga aagaagcgca
      241 cccggccaat acggcaatgg aaacggcggc aacagtccgc actgctattt tggataacat
      301 gattgagatc tcttgtgtga attaattgtt gattgatgtg atggatattt ttttgatctg
      361 acaccgcacc cggatttcag tgcagcaaac gtttgctccg gtcggacgcg tgtaagcgga
      421 tgagtccttg cagggctgtg tttgcgctgg ggtaaaaacg ggttgcggcg cagccaggtc
      481 agtatagaga acagccaggg gtcgttcaat tccggtatcg gttttggtca ggggcgaatg
      541 cagcaatcag gagcgaacgg acgggctgga gtcgtcagcg gcagcggtaa acagggtgct
      601 gcattttcgt ttatgagcga atccccgtgg cctgttgcgg cgctttcagg ctgatcagga
      661 tccgctggcg aggtcccatg gtgaggccct gaatgctgga ctctggcagc acggcctggt
      721 gcaggtggat tgtgtagcgt tccagcagat gggcgaggat cagtttcatt tcgtgcaacg
      781 cgaagccgta gcccaggcat tttttgtcgc cgccgccaaa gggcacaaat tcgttggcct
      841 tgtacttacg ccgcagaaag cgttcgggat cgaactggaa cggatgcgag tagaggtcag
      901 ggtctgtatg caggttgtga gtggcgacgc ccacatacgt gcctggccgg agcgggtgct
      961 gtcccagtgt gaatggctgc agcaccgagc gaatgaccag ggggaccacg ggccgtaacc
     1021 gcaacacttc cttgcagaag gcgtccagcc gctccgattt catcgcggca ctgttctgtg
     1081 tttcctgcgg ggctgcggcc tgttccccca gttgctggcg gatgctggtc tgtgcctgct
     1141 gatgagtcgc caggtagtaa agggcccagg ccagcgaatt ggcggtcgtt tcgtgcccgg
     1201 ccagcagcag agtggtgagc tggtcgcgga tatgaggcag gtctgcttcc gtcaatggcg
     1261 attggctggc gtgttgccgc agcaggtggc taaggatgtc gtcggcttcc cggccgggcg
     1321 cacgctggcg gatttccgca aagatcaggt catcgagtgt ctggcgcgct tgcagaaagc
     1381 ggtcccacgg gctgatgccg aacaaactga agcgggcttg tggcaccagc aacagcagcg
     1441 aggtaaatgc gttcagcaag cgttcgatag cggcgctgaa aagggcgcgc cggtcggggg
     1501 tgtcgatgcc gaaaaccgcg cgcagaatga catccagggt caccatcttc atgaatgcct
     1561 gtgcgtcgac agggctgctg agcgggtggc aggcatcgct gatcgcctgc cggatgaagc
     1621 cggaatagcg atgcaggcaa gcgccctgga acagcggcct gagttgctgc cgttcctgcg
     1681 tgtgggcccg gccctgcagc aggatcagcg aaccgttgcc cagcaatggc tgcagtgggc
     1741 tgggcaggga cgacgacagg gtgtcgctgt ccgcctgcag taactgactg acgagtgccg
     1801 gttcgctggt gaacaccacg tcgttcagtc cgggcatcag cacggtgaag gtattgccat
     1861 gccgggcgcg catgcgctgg aagaaccgtt ccacattcag gcccaggcgt gtggcgtggg
     1921 agagtcgggt aaaagatcgc atggcctgtt tatcggtggt cctgttcggg gagcaacggg
     1981 tgtttgtcat cagaatgcgc aagctgggaa gagcgcggtg cgacgatgtc ggcattc
//
LOCUS       DASUEG010000148         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1180607, whole genome shotgun sequence.
ACCESSION   DASUEG010000148 DASUEG010000000
VERSION     DASUEG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180607"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..704
                     /locus_tag="VFX11_02785"
     CDS             168..704
                     /locus_tag="VFX11_02785"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX4937570.1"
                     /translation="MHNWLIILLLLVSQTVAAETQNGTQPSTQPGTQPWEQLPESTRA
                     LLQNKAAQWNSLNPEQQQRLLQRAEKFRQLSPEQQQKALERFQRWQALPPERKQELRE
                     RYQKFRQLPPEQQKQIKERFRWFRSLPEAERQKLRESWQSLSPAERTELRERFRSASP
                     EQRRALRDQLLQRRLQRR"
     gene            720..1619
                     /locus_tag="VFX11_02790"
     CDS             720..1619
                     /locus_tag="VFX11_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937571.1"
                     /translation="MSSRTSRFTPITLLVIAMLMSTTRTLRAETPAWDLLLSAEHIHQ
                     EFDTSDADLDTLTFSPAVSLGSFSLSASLPWQKVSGVFFLDIARPNVASLCSLVQGLT
                     PLQKLRLVRNGTLTLAQLQYCNQYSGEVTSIEEKDSATGAGDIELFASYYLPPLTDTT
                     SASLGLGYKHDNGDADSGLGTGTRDLFAETALQLQPGPLRLLLTLGHNWLQHNDTGLA
                     LNDYGYGSLDAGVLLLQRLTLGIEYHYQQAGTDVTEDLDYLVWYGELDLPAGWGGRLF
                     LTDYLDADNLPREEFGGSLSWHF"
     gene            1736..2728
                     /locus_tag="VFX11_02795"
     CDS             1736..2728
                     /locus_tag="VFX11_02795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4937572.1"
                     /translation="MTEHNPHTAPSAAASKPGLITKEHLRAQLEDMAYRVLDPKEGLY
                     GPRSMAWKVNRHTEILLGAGCANLLQLAHPWVAQAIDQHSQTQTDPFGRLQRTFLNVH
                     SMVFGNLDQVLESALRVHNIHAKITGQVTESTGRTAKGSAYFANQIDAMLWVHATLWV
                     TGLRVYELFHKPLTSEEREQYYRESKLFAYLFGIPESAMPPSWADYLAYYNGVVNSDL
                     LAVGEVGRQLVEFIFSMRSSLVPILNRHKIHTAVLLPPSVRDAFGFPDVTPKVKASFD
                     FDVKLLGGVLRVAPKSIKYLPPYLEAQRRIAGGKAGPILRWTNQMIYGQPLLVS"
     gene            2898..3509
                     /locus_tag="VFX11_02800"
     CDS             2898..3509
                     /locus_tag="VFX11_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4937573.1"
                     /translation="MEVEIRAARPDDQGPIAELMYSSGPEIYDFLYKTRKTGAVDFIR
                     YEYGTGIGFCGHRNVTVAVKNGKVVGTGCFYDKTTYEKLISGSGKNFFGYFGIFGALP
                     VVWRSRHLGSTMKPPKDGELYLANFGVDPNLRSQGIGSAMIRHKIAEARQGGYKIFGL
                     DVAVTNPRGQALYTKLGLEVKKEKVFSGNDPNVPACRKMELAL"
BASE COUNT          762 a         1206 c         1003 g          613 t
ORIGIN      
        1 gaatacggcg atgccagcgg cgaactgttc gatccgctcg acctgggcga cgccgaagcg
       61 gcagcgcaac aggcccgtgc gcagcaaccc ctgatccgca ccggcaattc cgatcaggcc
      121 aacaaccgcc gccccgacgg cgccaccgaa tcagaggagt agaccccatg cacaactggc
      181 tcatcatcct gctgcttctg gtcagccaga ccgtcgcagc cgaaacgcaa aatggcacgc
      241 aacccagcac acaacccggt acacaaccat gggaacagtt gccggaatcc acccgcgccc
      301 tgctgcagaa caaggctgcg cagtggaaca gcctgaaccc ggaacagcag caacgcctgt
      361 tgcagcgcgc ggagaaattc cgccagctgt cgccggagca gcagcaaaag gcactggagc
      421 gtttccagcg ctggcaggca ctgccgccgg aacgcaaaca ggaactgcgc gaacgctatc
      481 aaaaattccg gcagctgccg cccgagcagc agaagcagat caaggagcgg ttccgctggt
      541 tccgctcgct gcccgaagca gagcggcaaa aactgcggga aagctggcaa tccctgtcgc
      601 cggccgaacg cactgaactg cgcgaacgtt ttcgcagcgc atcgccggaa cagcgccgcg
      661 ccctgcgcga ccagctgttg cagcgccgcc tgcaacgccg ctagaaaagg gatcaaacga
      721 tgtcatcccg aacgtcccgt ttcacaccca ttacgctgct ggtcatcgcc atgctgatga
      781 gcaccacccg caccctgcgc gcggaaacgc ctgcctggga cctgctgctg tccgccgaac
      841 acatccacca ggaattcgac accagcgacg ccgatctcga cacgctcacc ttctctcccg
      901 ccgtcagcct gggctccttc agcctcagcg ccagcctgcc ctggcagaag gtcagcgggg
      961 tcttcttcct tgatatcgcc cgccccaacg tggccagcct gtgcagcctg gtgcaaggcc
     1021 tcacgccgct gcagaaactg cggctggtcc gcaacggcac cctcaccctg gcgcagctgc
     1081 agtactgcaa ccagtacagt ggcgaggtca cctccattga ggaaaaggat tccgccaccg
     1141 gcgccggcga catcgaactg ttcgccagct actacctgcc gccactgacg gacaccacca
     1201 gcgccagcct cggcctgggc tacaagcacg acaatggcga cgccgacagc ggcctgggca
     1261 ccggtacccg cgaccttttt gccgaaaccg ccctgcaact gcagcccgga cccctgcgcc
     1321 tgctgctgac cctgggccac aactggctgc aacacaacga caccggcctg gccctcaacg
     1381 attacggtta cggctccctc gacgccggcg tgttactcct gcagcggctc accctgggca
     1441 tcgaatacca ctaccagcag gccggcaccg acgtgaccga ggatctggat tacctggtct
     1501 ggtacggcga actggacctg cccgccggct ggggcggccg cctgttcctg acggattatc
     1561 tggatgccga caacctgccc cgggaagaat tcggcggcag cctgtcctgg catttctgac
     1621 ggctggcaat tcatgacggg gcagtcacat tgatacaatt ttgatgatat gtatcaatga
     1681 aaacggcacc ggctctgcgt aacactgcag tcacaacacg agcaggagct gtcccatgac
     1741 tgaacacaac ccacacaccg cgccctccgc cgccgccagc aagccgggcc tgatcaccaa
     1801 ggagcatctg cgggcacaac tggaagatat ggcgtatcgc gtgctggacc ccaaggaagg
     1861 cctgtatggg ccccgctcca tggcctggaa ggtgaaccgc cacaccgaaa tcctgctggg
     1921 cgccggctgt gccaacctgc tccagctggc gcatccctgg gtagcccagg ccatcgacca
     1981 gcactcgcaa acccagaccg acccgttcgg ccgcctgcag cgcaccttcc tcaacgtgca
     2041 ctccatggtg ttcggcaatc tggaccaggt gctggaatcc gccctgcgcg tccacaacat
     2101 ccacgcgaag atcaccggcc aggtcaccga gagcaccggc cgcaccgcca agggcagcgc
     2161 ctatttcgcc aaccagattg acgccatgct ctgggtacac gccacactgt gggtcaccgg
     2221 cctgcgggtg tatgaactgt tccataagcc cctgaccagc gaagaacggg agcagtacta
     2281 ccgcgaatcc aaactgttcg cctacctgtt cggcattccc gaaagcgcca tgccgccctc
     2341 ctgggccgac tacctggcct actacaacgg cgtggtgaat tccgatctgc tggccgtggg
     2401 cgaagtaggc cggcaactgg tggaattcat tttcagcatg cgctccagcc tggtgcccat
     2461 cctgaaccgc cacaagatcc acaccgccgt gctgctgccg ccatcggtac gggacgcatt
     2521 tgggttcccc gacgtcacgc ccaaagtgaa ggcaagcttc gacttcgacg tgaagctgct
     2581 ggggggtgtt ctgcgcgttg cgccgaaaag catcaaatac ctgccaccct accttgaagc
     2641 ccagcgccgc attgccggtg gcaaggccgg cccgatcctg cgctggacca accagatgat
     2701 ctacggccag ccgctgctgg tgtcctgaac ctgccgccga ctctttgcgg ttggtgcaat
     2761 cctgttgtca tggcttatgt ccattcatgc cgcttgtgtc gcaccgtcgc acaggcggtt
     2821 ttttatggcc gcaatggaat tgcgcacggc tggcgttcat gtcaaattgg cgagccttgt
     2881 tcctgcagga aaatcccatg gaagtggaaa tcagagccgc ccgccccgac gatcagggcc
     2941 ccattgccga actgatgtac agctcaggcc cggaaattta cgattttctt tacaagacgc
     3001 gcaaaaccgg cgcggtggat tttatccgtt acgaatacgg caccggcatc ggcttttgcg
     3061 gccaccgcaa tgtcacggtg gcggtgaaga acggcaaagt ggtaggcacc ggctgctttt
     3121 acgacaagac cacctatgaa aaactcatct ccggttccgg caagaatttt tttggttact
     3181 tcggcatctt cggcgcacta ccggtggtat ggcgctcccg ccatctgggc agcaccatga
     3241 aaccgccgaa ggacggcgaa ctgtacctgg cgaatttcgg cgtcgatccg aatctgcgca
     3301 gccagggcat cggctccgcc atgatccggc acaaaatcgc cgaggcgcgt caagggggtt
     3361 ataaaatctt cgggctggat gtggctgtca ccaatccgcg cggccaggcg ctgtacacca
     3421 aactggggct ggaagtgaaa aaggaaaagg ttttttcggg gaatgatccg aacgtgccgg
     3481 cgtgcaggaa gatggagttg gcgctgtagt ggatcgaaga caattaaccg atgcgccgca
     3541 acaacaaccg ccgcatcggt tccggatctt tctgcttcac cgcc
//
LOCUS       DASUEG010000149         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2017294, whole genome shotgun sequence.
ACCESSION   DASUEG010000149 DASUEG010000000
VERSION     DASUEG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017294"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VFX11_02805"
     CDS             <1..1906
                     /locus_tag="VFX11_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX4937574.1"
                     /translation="LQRELSYRPSDRHSIEQISEVDHLNRGQLVQALATVLTAPGNES
                     HQTIGLLGDWGVGKSTLVHLLKNELVRRQEQQPFLFAEFNAWAYEHTDNLQAGIAQEM
                     LKALSSDMPRPKGDVAPAATLPAKVRRWWDGFFSQSLWLCKRVLITCRFAIALNGWRM
                     LWLLLMLLVAVLPFTWGDVGETLVGLATESGTTSAGDAVAATLGTGIWVAGFIYYFVT
                     RLKPVLANPFAKELLTYLKLPDYGEHLGAIPVMRKNIETLCRVRLKSQRRHHKDKRLL
                     FVVDDLDRCGHEGIVKVLEAVRLVLDLKRVTVIIAVDQRIALAALALHYKDLAEHHQL
                     QNPRAIARDYLAKVIHLPILLSPPDDKSIEGYLNHIWQEQTGDVSVALSTPEQKPVAP
                     TSSADASAGNARPVQQANAAPPRESTPANGTAPVTDAGLTPAGNVIPLRPSVNASTAS
                     NASAATPDTGNNPAPSPDKPAPLVVPGLSTLQKQAFLHWVKHFHLTNPRQLKRLHNSY
                     NLLRHFYGEDNAGPAPATVAELVKTLEFPLMVTLFALECLNSLDDPPRRAQLKARLRG
                     RSEKPFSNEAEPEADGQPFSAAVIALVNRRMPVTQVHLVDAVEPFVLPAIEQDVVRES
                     QAQSEHTKQA"
     gene            complement(1959..3308)
                     /locus_tag="VFX11_02810"
     CDS             complement(1959..3308)
                     /locus_tag="VFX11_02810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4937575.1"
                     /translation="MTAVAPVRANTSIAGAWAPGKNLAALFARQQDAFDLINGLRSMS
                     FLWILIFHTCYSYGMVAGKDSVFALSESSPLLWWIFNADKAVDLFFILSGFLISLILF
                     KEVNRTGTIQLGRFYFRRYLRLTPVYALILFLYWLSGGRNHEYVWANLFYVNNFLELD
                     QMALQWTWTLAVEEQFYLLLPLLLIVIARMPGQPFLRVLGGLLALSFVARLAAFYFLP
                     DMWSSDYRALLTDKEICPTFYTNIYDNLITRYGPFVCGTFAACAYCFHREQLTTWLLT
                     HRRTFFAINLLMATLTLFFLFFPVTNQVLEGGSWQLRTYLVVNRTLFSAGIAWFMLTV
                     FLQLHNGHWLARALSIRLWQPFSQLTYSMYLVHMIVIIGVMFQINYLFNHQLAIADAT
                     WRIFATLTVTATLSLMITMLIGVLCWLLVEKPFLNLRDRAATSRGATTAAPIQHSLG"
BASE COUNT          728 a         1001 c         1053 g          617 t
ORIGIN      
        1 gctgcaacgg gaactttcct accgcccctc tgaccgccac agcatcgagc agatcagcga
       61 ggtggaccat ctcaaccggg gccagctggt gcaggctctg gccaccgtgc tgacggcgcc
      121 cggcaatgaa agccaccaga ccatcggcct gctgggcgac tggggcgtgg gcaaatccac
      181 cctggtgcat ctgctgaaaa acgaactggt gcggcggcag gagcagcagc cgtttctgtt
      241 tgccgaattc aatgcctggg cctatgagca caccgacaac ctgcaggccg gcatcgccca
      301 ggaaatgctg aaagcgttgt cgagcgatat gccacggccg aaaggtgatg tcgcgcccgc
      361 tgcgacgctc ccggcgaaag tgcggaggtg gtgggacggc tttttttcgc aatctctctg
      421 gctgtgcaag cgggtcctga tcacctgccg gttcgccatc gcgctgaacg gctggcgcat
      481 gctctggctg ctgctgatgc tgctggtggc ggtgctgccg ttcacctggg gcgatgtggg
      541 cgaaacactg gttggcctgg cgactgaatc gggtacaact tctgcgggcg atgccgtggc
      601 ggccacgctg ggcacaggca tctgggtggc gggtttcatc tactatttcg taacccgcct
      661 caagcccgtg ctggccaatc cctttgccaa ggaattgctg acctatctga aacttcccga
      721 ctatggcgag cacctcggtg ccattccggt gatgcgcaaa aatattgaaa ccctgtgccg
      781 ggtgcgactg aaatcgcaac gccggcacca caaggacaag cgcctgctgt tcgtggtgga
      841 cgatctggat cgctgcggcc atgagggcat cgtgaaagtg ctggaagcgg tgcggctggt
      901 gctggatctg aagcgcgtga ccgtgatcat cgccgtggac cagcgcattg cactggcggc
      961 actggcgctg cattacaagg atctggcgga acatcatcaa ctgcagaatc cccgcgccat
     1021 tgcccgcgac tatctggcca aggtcattca tctgccgatt ctgctgagcc cgcccgatga
     1081 caagtctatc gaaggctacc tcaatcacat ctggcaggag cagaccgggg acgtcagcgt
     1141 ggcgctgtcg acgccggagc aaaagccggt tgcgccgacg tccagtgcag atgcatctgc
     1201 tggcaacgcc cgtccggtgc agcaggcaaa cgccgcgccg ccgcgcgaat ccacgccggc
     1261 aaacggtacg gcaccggtga cagatgccgg gctcactccc gcgggcaatg taatcccgct
     1321 gcgtccgtcg gtgaatgcat caacagcatc gaatgcgtct gcggccacgc ccgacaccgg
     1381 caacaatccg gcgccgtcac ccgacaagcc cgctccgctg gtggtccccg ggctgagcac
     1441 gctgcagaag caggcctttc tgcactgggt aaaacatttt cacctgacca atccgcgtca
     1501 gctcaaacgg ctgcacaaca gctacaacct gctgcggcat ttttacggcg aggacaatgc
     1561 cggcccggca ccggccacag ttgccgagct ggtgaaaacg ctggaatttc cgctgatggt
     1621 gaccttgttc gcgctggagt gtctcaacag cctggacgat ccaccgcggc gcgcgcaact
     1681 gaaagcccgc ctgcgcggac gcagcgaaaa accgttcagc aacgaggccg aaccggaggc
     1741 cgacggacag ccgttcagtg ccgccgtgat cgcgctggtg aaccggcgca tgcccgtcac
     1801 ccaggttcat ctggtggatg cagtggagcc cttcgtattg ccggctatcg aacaggatgt
     1861 ggtgcgggaa tcgcaggcgc agtcggaaca cacaaaacag gcgtgaagac gctgccgttg
     1921 cggcagcgcc tgtcaaacct tttggcttgc gcctgctgtc atcccagcga gtgctggatg
     1981 ggtgccgccg tggtggcgcc gcggctggtg gcagcgcggt cgcgcagatt caggaacggc
     2041 ttctccacca gcaaccagca caatacgccg atcagcatgg tgatcatcag cgacagtgtc
     2101 gccgtgacgg tcagcgtggc gaaaatccgc catgtggcat ccgcgatcgc cagctggtgg
     2161 ttgaagagat aattgatctg gaacatcacg ccgatgatca cgatcatgtg caccagatac
     2221 atggaatagg tgagctggct gaacggctgc cacagccgga tggacagcgc ccgcgccagc
     2281 cagtgaccgt tgtgcagttg caggaacacg gtcagcatga accaggcaat gcccgccgaa
     2341 aacagtgtgc gattcacgac cagatacgtc cgcagttgcc atgaaccgcc ttccagcacc
     2401 tgattggtca ccggaaagaa caggaagaac agtgtcagcg tggccatgag caggttgatg
     2461 gcgaaaaacg tgcggcgatg cgtcagcagc caggttgtca gttgttcgcg gtgaaaacag
     2521 taggcgcagg ccgcgaaggt gccgcacacg aacgggccgt agcgggtgat caggttgtcg
     2581 taaatattgg tgtagaacgt cgggcagatt tccttgtcgg tgagcagggc gcggtagtcg
     2641 ctgctccaca tgtcgggcag aaaataaaac gcggccagcc gggccacgaa cgacagcgcc
     2701 agcaatcccc ccagcacccg caggaacggc tgtcccggca tacgggcgat gacgatcagc
     2761 agcagcggca gcagcagata gaattgttcc tccactgcca gtgtccaggt ccactgcagc
     2821 gccatctggt ccagctcaag aaaattgttc acgtaaaaca ggttggccca gacgtactcg
     2881 tgattgcgcc cgcccgacag ccagtacaga aataatatca gggcatacac gggggtcagg
     2941 cgcagatagc ggcggaaata aaaacgtccc agctggattg tgccggtgcg attcacttcc
     3001 ttgaacagga tcaggctgat aagaaagcca ctgagaatga aaaacagatc cacggccttg
     3061 tcggcattga atatccacca cagcagggga gagctttccg acagtgcgaa cacactgtcc
     3121 ttgccggcca ccatgccata ggaatagcag gtgtggaata tcaggatcca caggaagctc
     3181 atgctgcgca ggccgttgat caggtcaaat gcgtcctgct ggcgggcaaa cagcgcggcc
     3241 aggttctttc cgggggccca ggcaccggca atgctggtgt tggctcgcac aggggcgacg
     3301 gcggtcattg cgtttccttt tttgttattt ggtttgggcg gtcgccagtt gattctagcc
     3361 ggatgattct agccggatga ttctagaaag gaactaacg
//
LOCUS       DASUEG010000150         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1078493, whole genome shotgun sequence.
ACCESSION   DASUEG010000150 DASUEG010000000
VERSION     DASUEG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078493"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFX11_02815"
     CDS             complement(<1..232)
                     /locus_tag="VFX11_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937576.1"
                     /translation="MNRIPMLLPRVLMAVLLMLLNSVAQALPAEIEADRLVLAAEEKI
                     AQQDFDGARQYLERVAPLKVEPKPVYYYLYGQV"
     gene            complement(348..707)
                     /locus_tag="VFX11_02820"
     CDS             complement(348..707)
                     /locus_tag="VFX11_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4937577.1"
                     /translation="MEKRIREKRVIKRHHLRYYLSVYNRKTGKPIGYIVNISTEGMRL
                     VSHIPLLTHSVFQFRIKLPREIEGARNIDFDALSCWCRPDVSPDCFDTGFKLIDAPQE
                     LLDLIEGLTQYFSFKLD"
     gene            976..>2537
                     /locus_tag="VFX11_02825"
     CDS             976..>2537
                     /locus_tag="VFX11_02825"
                     /inference="COORDINATES: protein motif:HMM:NF026068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937578.1"
                     /translation="MRITLPLLVLCFFCQFCLPARAANLLPITDAQEKQWESQRAAYR
                     QTLAMLGKKPRPEYQAAAEKLKDYALYPDLKYREYSHYIGAVSRQEVDNYIARFGDSI
                     LATRLRQQYVQMQAIQQNWRNYLAEYQPGQYGARFECQYYWALHQTGKRDQAFAGARK
                     LWLVGTPQDKACDPLFTAWKSAKGITSSLAWERTRLAMEQQQYQLAKNLEVYLPKQQQ
                     ALSREWRELVRDPKRLRDSSRYRALGDAARPLLVTGFSRLIRQNSALALQLWPQYERM
                     FRFSTDEKRAVTGEFAYVLGANQAPEAGYWLVQAAQYAGNEHLAPMAVRTALRQRDWP
                     QARRSLALLGEKDRQLQDWRYWIARADQHVGPLDPANQPRIRTRDNRRDVLGFHQRYL
                     NALYNPYSTFTLLPESVLRQVFPIQKPEETFKTISRERSFYGFLASERLKQPLQLNQI
                     TSQVTEEELRALTRKAGIRRARELYLLGETFQSRLEWHHMINRMSPKERGAAAHLAYI
                     WGWHNPALLAAV"
BASE COUNT          540 a          810 c          729 g          458 t
ORIGIN      
        1 gcacctggcc gtacagatag tagtagacag gcttgggttc cactttcagt ggcgcaaccc
       61 gctccagata ctggcgggca ccatcaaaat cctgctgggc aattttctct tccgccgcca
      121 gcaccagacg atcggcctcg atctcggcgg gaagggcctg cgcaacgctg ttcagcagca
      181 tcagcaacac agccatcagc acgcgcggca gcaacattgg aattcggttc ataggacgct
      241 ttcgcttgac ggatgaaacg gaacaaaaat cagacaatgg aagggattct gacaacaggg
      301 aggcgcgggc gcaagccggc cgtgtccctg gccagcggaa gtgtgaatca atcgagcttg
      361 aagctgaaat actgggtcag gccttcgatc aggtccagca gttcctgggg cgcgtcgatc
      421 agcttgaagc cggtatcgaa gcagtcgggg ctgacatccg gccggcacca gcagctgagg
      481 gcgtcaaaat cgatgttgcg tgcgccctct atttcccggg gcaacttgat gcggaactga
      541 aacacgctgt gcgtcagcaa cggtatatgg ctgaccagac gcatcccctc ggtggaaata
      601 ttgacgatgt aaccgatggg cttgcctgtc ttgcgattgt aaacgctcag gtagtaacgc
      661 agatggtggc gtttgatgac ccgtttttct ctgattcgct tttccataat ccccggccgc
      721 gcaaaaaaca gtctgacatg aatttaagtt tagcgttccc cgccgggtcc acaaccgcgt
      781 cagccgtttt tttactgccg gccccggtgt caggataccg tcacgcccgt tgcggcccat
      841 acgcaaggcc atttagcgca ggtgaaataa gcagctgaaa attattgaaa tgtattcccg
      901 ggactggttc ataatggatg gcaattttta tcgccccatc tacgcttgag ttagccattc
      961 gttgccagta tgtccatgcg tataacgtta cccttattag tattgtgttt cttctgtcag
     1021 ttttgccttc cagcccgtgc cgccaacctg ctgccgatca cggatgccca ggaaaaacag
     1081 tgggaatccc agcgtgctgc ctaccgccag accctcgcca tgctgggcaa gaagccccgc
     1141 cccgaatatc aggctgcggc ggaaaagctg aaggactacg cgctctatcc tgacctgaaa
     1201 tacagggaat acagccacta catcggtgcc gtcagccggc aggaagtgga caactacatc
     1261 gcccgcttcg gcgattccat cctggccacc cgcctgcgcc agcagtacgt gcagatgcag
     1321 gccatccagc agaactggag aaactacctg gcggaatacc agccggggca gtatggcgcc
     1381 cgcttcgaat gccagtacta ctgggccctg caccagaccg gcaagcgcga ccaggccttt
     1441 gccggcgcgc gcaaactgtg gctggtggga accccccagg acaaggcctg cgatcctctg
     1501 ttcaccgcct ggaaatccgc caagggcatc accagcagcc tggcctggga acgaacccgg
     1561 ctggccatgg aacagcagca ataccagctg gccaaaaatc tggaagtcta cctgcccaaa
     1621 cagcaacagg ccctgtcgcg ggaatggcgg gaactggtgc gggacccgaa acggctgcgc
     1681 gacagcagcc gctaccgggc actgggcgac gccgcccggc cgctgctggt gaccggcttc
     1741 tcgcgcctga tccggcaaaa cagcgccctg gcgctgcagc tgtggcccca gtacgaacgc
     1801 atgttccgct tcagcaccga cgaaaagcgg gctgtcaccg gcgaattcgc ctacgtgctg
     1861 ggtgccaacc aggctcccga ggccggttac tggctggtgc aggccgccca gtacgcgggc
     1921 aacgaacacc tggcacccat ggccgtgcgc accgccctgc gccagcgcga ctggcctcag
     1981 gcccgccgct cgctggcact gctgggggaa aaggatcgcc agctgcagga ctggcgctac
     2041 tggatcgccc gggcggatca gcatgtcggc ccgctggacc ccgccaatca gccccgcatc
     2101 cgcacccgcg acaaccgccg cgacgtgctg ggctttcacc agcgctacct gaatgcgttg
     2161 tacaaccctt actccacctt cactctgctg ccggaatccg tcctgcgcca agtgttcccg
     2221 atccagaagc ccgaggagac tttcaagacc atctcccgcg aacgcagctt ctacggcttc
     2281 ctcgccagcg aacgcctcaa gcagccgctg cagctgaacc agatcaccag ccaggtgacg
     2341 gaagaggaac tgcgcgccct cacccgcaag gccggcatcc gccgggcgcg ggaactgtat
     2401 ctgctggggg aaacctttca gtcgcggctg gaatggcatc acatgatcaa ccgcatgagc
     2461 cccaaggaac ggggcgcagc ggcgcatctg gcctacatct ggggctggca caatccggcg
     2521 ctgctggcgg cggtaca
//
LOCUS       DASUEG010000151         8637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_101774, whole genome shotgun sequence.
ACCESSION   DASUEG010000151 DASUEG010000000
VERSION     DASUEG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101774"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFX11_02830"
     CDS             complement(<1..976)
                     /locus_tag="VFX11_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937579.1"
                     /translation="MKTNSLLRCLILLFGFTLLQNAVALPKQNDNGDPNDLPEDPRPA
                     APTVTPRRMDNDKLLFGVNGIKVNWKDASYNETGFRVFRQREGTTDWTKIKTLAAIDG
                     SGNFRNFMDNSVEWDTRYCYKIEAYNANGRRESAPRCVKTSIEGINPPVVRAQLRIDV
                     GEHGGGHEGTNNILGVGLPGYTGLYPAHAEGTSLADLIQGAVIRRGSSRLYDLNFYKV
                     KYVGDLEDFRLSISGQDDICIKKLALYVNGVELFNHPMRDADSDCKHFRGTNDIYNLI
                     YTVPRQDMRSSPGWAHVRSNIYVNPTEHNAGVTDFDNRFPWRSWVFRDE"
     gene            complement(1127..2197)
                     /locus_tag="VFX11_02835"
     CDS             complement(1127..2197)
                     /locus_tag="VFX11_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937580.1"
                     /translation="MNNWHEWMRQLLGLIGLPASLAGSYVNAFIPVARQLYDIDPYAI
                     LRAVDIQPEQLAQPGFRLSLYRTFKLLKIAEDVTGDPLVGLRLGQHVQPKSYEVLGYA
                     LMSRSNLQQAIEQLIRFDALIWDVGGTRFFREGKFAVLELNPLYVPWVPPQAFELAIS
                     GQVAFGRWITGFEGAPFAVSFRHACRGDHAVYADVFGCDVLFDAPRNAVIFPEEYLQM
                     PLRYADPTLARIMIGTGQRLMENYRQETNIANETRALICELLPTAQQDMDSVAQRMGM
                     SGYQLSRRLQGAGYRFPDLVEEVRQDLAGHYLASADLALTDIAFLLGFSEQSAFTRAF
                     KRWTGQAPAHFRRQQLAESTVA"
     gene            complement(2343..4196)
                     /locus_tag="VFX11_02840"
     CDS             complement(2343..4196)
                     /locus_tag="VFX11_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX4937581.1"
                     /translation="MTLAADNVGAVAPQYRFLTFLVFGAIIALTMFVTYRAAKRVHSA
                     SDFYTAGGGISGLQNGWAIAGDYLSAASFLGIAGLISLYGYDGFMYSVGFLVAYITVL
                     LVIAEPCRNIGKYTLADILAYRNDPKKTRVAGALSVITVSTIYLTAQMVGGGVLVKTL
                     IGIEYEISVVVVGILMLAYVLFGGMVATTWVQIIKAILLVVASLILVVLVWAPYGFSL
                     PGFLGAAVSDPKVQAQVARLIGDPASVMTAEQLGQRFLEPGLYLKDPLDQISLGLALV
                     LGTAGLPHILMRFFTVPTAQEARKSVIWAMAIIGGFYVLTIFLGLGSAINVGAPAISA
                     IDKGGNMAAPLLAQYLGGGADSLLGNLFLAFVAAVAFATIVAVVAGLVLAAASAIAHD
                     IYVGVIRDLHVTPQEQVRAARISTVVVGVVAIAIGIAAKGQNVAHLVGLAFAVAASAN
                     LPCIVLTLYWRRCNTGGIIAGMVVGTFSAIAMVLVSPNMTYPNKVRTDSEKIVATATV
                     ERDALTQRFANASAEEQVAMQTRLDKLQKDLAKAQEDLQKVAGQTTSMVGLEQPLVPL
                     KNPGIVSIPLGFLAVFIGSLLWRRDPRADALWDEVYVRQNTGIGAAKAQQH"
     gene            complement(4231..4575)
                     /locus_tag="VFX11_02845"
     CDS             complement(4231..4575)
                     /locus_tag="VFX11_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX4937582.1"
                     /translation="MSQPPPIDWAAIEADPRFQHLHRRKNRFLWSLMGFSLCYYFLLP
                     LGSAYTPELFRQQVWGPLNVGLLFALSEFIVAWGVAVLYSRRANREFDVLAAEIVAAY
                     SPAKASAGARHE"
     gene            4847..5488
                     /locus_tag="VFX11_02850"
     CDS             4847..5488
                     /locus_tag="VFX11_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX4937583.1"
                     /translation="MRIVAILLGLLAALSTSTVAMADDYREGEHYVVLQPAVRTADPG
                     KIEVAEVFAYSCPHCFHFEPLLQAWEKKQAADVYLMQVHANWNSQMEPLIRGYYTSVA
                     LKIRDQTHMAVFNALHLEHKNLTSAEQWADFFAPFGIDKAKAISTYNSFGVASQVKQA
                     DARTRQYQVRGTPELIVNGKYRVSSRLTGGQEEMLKVADFLVAKERAARQGSQ"
     gene            5656..7866
                     /locus_tag="VFX11_02855"
     CDS             5656..7866
                     /locus_tag="VFX11_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937584.1"
                     /translation="MKTNHHGLQGLRTLAALCLTLLLTACGGSDIQFQTPAKNAVYGS
                     ADLIDFDIRFVKTLPADATFKLNGTDVTYQFAVDNTAGTATAQLAASTILDEKNRLEV
                     STPQVTRTLVFYVDRTGPEFHITNIDPWLMLPGVTVTFSGYVTDLSPINTSGAPGTLP
                     PALWVKTGDASYRSVAPITLNENNEFSVTYQLEADPSGVVGEFLPLVFRAEDIHRIRS
                     NDAFGSIVNVPQLAKAKVTSNAFANVITPAAETAVNALNLEQLIKARNPVVDKGWSIF
                     SFKLDVTNFELDDLDLAILPTSEGGYQVRANVNAQNLDVSIWSRVLADVYLGTIGVGV
                     PTGLHNADVTGNVLVKFRVDGNPNANPTLVVDHIEPNLNIIGGDFDAIDFGFPIDLPG
                     LGTLEQLIFEAIDSSIAELIGEKVGEKVADKINDQLKLIPTDFDITLRNKTLNFYLMT
                     ASVTGDSTGLEVAVTEADALPPVNQRDPSVIREPGYHIHDRSAVLPGFSATTPNGQPF
                     DVGLSLDWDILNKTIYSAHLTGIDKLSTTVAGSSIPKLGTILKNYEMKLDVVPLYSPY
                     LDLPLPRTTPALASVQAKDVQITAQVRRTDIEDTFHPLIQIVANVRADIGLGLSGNRI
                     RVLLDTDPRIDIDSVVKEQSSIVLDEQFIQALVDFAIPKVMPRLADAIQEIRLPTVSG
                     YTLNLLEAENIGNRFFKVYARVQQGNTTGGGGGICCIGGVGGIGGVLEAELATQ"
     gene            complement(7992..8333)
                     /locus_tag="VFX11_02860"
     CDS             complement(7992..8333)
                     /locus_tag="VFX11_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX4937585.1"
                     /translation="MKIQEITMPERIMLAEALWDSLEDQGSEFDVTDEQAKELDRRLA
                     GFEIDNDECSDWTSVRSGLVFTSCAIEPTLRKPIRRFPRCIYCLVQQNKIIVTAVFRV
                     REDPGSWSNRT"
BASE COUNT         1917 a         2647 c         2436 g         1637 t
ORIGIN      
        1 gttcatcgcg gaatacccag ctgcgccagg ggaaacggtt gtcgaaatcg gtgacgcccg
       61 cattgtgctc ggtgggattc acataaatat tgctgcgcac gtgcgcccag ccgggcgagc
      121 tgcgcatatc ctggcgcggc acggtgtaga tcaggttgta gatgtcgttg gtgccgcgaa
      181 aatgtttgca gtcagaatcc gcatcgcgca tcggatgatt gaacagctca acaccgttca
      241 catagagcgc cagttttttt atgcagatat cgtcctgccc ggaaatcgac agcctgaaat
      301 cttccagatc gcccacgtac ttcaccttgt agaaattcag gtcgtacagg cgcgagctgc
      361 cgcgacggat caccgcaccc tggatcagat ccgccagcga tgtgccttcg gcatgggccg
      421 gatacagtcc ggtgtagccg ggcaagccca cgccgagaat attgttggtg ccttcgtggc
      481 cgccgccgtg ttcacccacg tcgatgcgca gctgggcgcg caccacgggc ggattgatgc
      541 cttcgatgga cgtcttgacg cagcgcggtg cgctttcgcg ccggccgttc gcgttgtagg
      601 cctcgatctt gtagcagtag cgggtatccc attccacgct gttgtccatg aagttgcgga
      661 agttgccgct gccatcaatg gcggcgaggg ttttgatttt ggtccagtcg gtggtgcctt
      721 cgcgctggcg aaacacgcgg aagccggttt cattgtacga cgcatccttc cagttcacct
      781 tgatgccatt cacgccgaac agcagcttgt cgttgtccat gcggcgaggc gtgacggtgg
      841 gagctgcggg gcgcgggtct tccggcagat cattgggatc accgttgtca ttctgtttgg
      901 gcagggcaac ggcgttttgc agcagcgtaa aaccgaacaa caatatcagg cagcgcagga
      961 gtgagttcgt tttcatggcg caactccatg ggtagtggcg aagtgggagt gttcacagct
     1021 ccactctaca aacgctgcat cgacaatgct tgactgtgcc acactcggca atcgcgccgt
     1081 gttgtcaaat tttttggagt aattcgcaac cacagaaaat acaaaatcag gcaaccgtgg
     1141 attccgccag ctgctgacgg cggaaatggg ccggcgcctg gccggtccag cgcttgaacg
     1201 cgcgggtgaa ggcgctctgc tcggaaaaac ccagcaaaaa cgcgatatcg gtgagagcga
     1261 gatctgcact cgccaggtag tgtcccgcca gatcctgccg cacttcctcc accagatccg
     1321 ggaaacggta ccccgcccct tgcagccgcc gcgacagctg gtaaccggac atacccatgc
     1381 gctgcgccac actgtccatg tcctgctgcg ccgtgggcag cagttcgcag atcagcgcgc
     1441 gggtttcgtt ggcaatgttg gtttcctgac gataattttc catcaggcgc tggccggtac
     1501 cgatcatgat ccgcgccagc gtgggatcgg cgtagcgcaa cggcatttgc aggtattctt
     1561 cgggaaaaat cacggcgttg cggggcgcgt cgaacagcac atcgcaacca aacacgtcgg
     1621 cataaaccgc atgatcgcca cggcaggcgt gacggaagct gaccgcaaac ggcgcgccct
     1681 cgaaaccggt gatccagcgc ccgaacgcca cctgcccgct gatcgccagc tcgaatgcct
     1741 gcggcggcac ccagggaaca tagagcggat tcagttccag cacggcgaat ttgccttcgc
     1801 gaaaaaaacg ggtgccaccc acatcccaga tcagcgcgtc gaaccggatc agctgctcga
     1861 tggcctgctg caggttgctg cggctcatca gcgcataacc cagcacttcg taggatttcg
     1921 gctgcacatg ctggcccagg cgcagtccca ccagcgggtc gccggtcaca tcctcggcga
     1981 ttttcagcag tttgaaggtg cgatagaggg aaagacggaa gccgggctgg gccagctgct
     2041 ccggctggat atcgacagcg cgcagaatgg cataaggatc gatgtcgtac agctgtcgcg
     2101 ccacgggaat aaaggcgttg acgtaggaac cggccaggct ggccggcaat ccgatcaggc
     2161 ccagcagttg tcgcatccat tcatgccagt tgttcatggg aatccctgcg cccggcgcgt
     2221 aagtggcagt gaacgctagt gtgcgccgcc agaggcaggg attccgttaa ttattacggc
     2281 cacagcatcc gtaatttgtg caaccggttt tgcgctgcag tacgggtgag cagcggggcc
     2341 ggtcagtgct gctgggcttt ggcggcgccg atgcccgtgt tttgccgcac atacacttcg
     2401 tcccacaagg catcggcacg gggatcgcgg cgccacaaca gcgaaccgat gaacaccgcc
     2461 agaaagccca atggaatcga cacgataccg ggatttttca gcggcaccag cggctgttcc
     2521 agccccacca tcgaggtggt ctgtccggcc actttctgca gatcctcctg cgccttggcc
     2581 agatctttct gcagtttgtc gagccttgtc tgcatcgcga cctgttcttc cgcgctggcg
     2641 ttcgcaaaac gctgcgtcaa cgcgtcgcgt tccactgtcg cagtggcgac gattttctct
     2701 gaatcggtgc gcaccttgtt ggggtacgtc atgttcggcg acaccagcac catcgcgatg
     2761 gcactgaagg tgcccaccac catgccggcg ataatgccgc cggtattgca gcgccgccag
     2821 tacagcgtca gcacgatgca aggcaggttg gcactggcgg ccacggcaaa cgcgaggcct
     2881 accagatgcg ccacattctg gcctttggca gcaatgccga tggcgatcgc gacgaccccc
     2941 accaccaccg tggaaatgcg cgccgcccgc acctgttcct gcggcgtgac atgcagatca
     3001 cggatgacac ccacgtaaat gtcatgggcg atggcactgg cagcggccag caccagccct
     3061 gccacaaccg cgacgatagt ggcaaaggcc acggccgcca caaaggcgag gaacaggttg
     3121 cccaaaaggg aatccgcacc gccacccaga tattgcgcca gcaacggcgc tgccatgttg
     3181 ccgcccttgt cgatggcgga aatggcgggc gcacccacgt tgattgcaga acccaggccc
     3241 agaaaaatcg tcagcacgta aaagccgccg atgatggcca tggcccagat caccgacttg
     3301 cgggcttcct gcgcggtggg cacggtgaag aaacgcatca gaatatgcgg cagtccggcg
     3361 gtacccagca ccagcgccag tccgagggaa atctggtcca gcggatcttt cagatacaga
     3421 cccggctcca ggaaacgctg gcccagctgt tccgctgtca tcacactcgc cggatcaccg
     3481 atcagccgcg ccacctgcgc ctgcactttc ggatcggaca cggccgcacc cagaaaaccc
     3541 ggtagcgaaa atccgtacgg tgcccacacc agcaccacca gtatcaacga cgccaccacc
     3601 agcagaatgg ccttgatgat ctgcacccag gtggtggcca ccatgccgcc gaacagcaca
     3661 taggccagca tcaggatgcc caccaccacc actgaaattt cgtactcgat gccgatcagc
     3721 gttttcacca gcacgccacc gcccaccatc tgcgcagtca gatagatcgt cgacaccgta
     3781 atcaccgaca gcgcaccggc gacgcgggtt ttcttgggat cattgcgata ggcgagaata
     3841 tccgccagcg tgtatttgcc gatattgcgg cacggctcgg caatcaccag caacactgtg
     3901 atatacgcca ccagaaatcc taccgaatac atgaagccgt catagccata gagggaaatc
     3961 aggccggcaa taccgagaaa cgacgctgcc gacagatagt ccccggcaat cgcccagcca
     4021 ttctgcaaac cggaaatgcc accaccggcg gtgtagaaat ccgatgccga atgcacgcgc
     4081 ttggcggcgc ggtaggtaac aaacatggtg agggcgatga tcgcaccgaa caccagaaac
     4141 gtcaggaagc ggtattgcgg cgcgaccgcg cccacgttgt ccgctgccag tgtcatgccg
     4201 ggcagcagca tcaggaacag cagcagggat ttattcatgg cgggctcctg cgctggcctt
     4261 cgcaggggaa taggccgcca caatctcggc ggcgagcaca tcgaattcac gattggcgcg
     4321 gcgcgaatac agcaccgcca caccccaggc cacgatgaat tccgacagcg caaacagcaa
     4381 ccccacattc agcggccccc acacctgctg ccggaacagt tccggcgtgt aagccgaacc
     4441 cagcggcaac agaaagtagt aacagaggga gaagcccatc aggctccaga ggaaacggtt
     4501 cttgcgccgg tgcagatgct ggaaacgggg atcggcctcg atggcggccc aatcgatggg
     4561 gggtggctgg ctcatatcgc tctcctgctg aaaaaaatcc ttgccacaag tctgtgccat
     4621 ggaaggtgcg ccagcaagtt tccgtggcaa tcaagcctgt ctatacgacg acctggcgaa
     4681 tgcggctttc aagtgactga ttttcatggc gacaaaaaac gctccaccca ggtgccggtt
     4741 gcgatcggct ctttacagaa ggtgcaaggc accagagaat ccattacccg cgggaatcgc
     4801 ggcattcccc attcgatttc agacaagcac ccatcaagga gcgtttatgc gcatcgtggc
     4861 aatactgttg ggcctgctgg ctgccctgag caccagcaca gtggcaatgg ccgacgacta
     4921 tcgggaaggc gagcactatg tggtactgca gcccgccgtg cgaaccgcgg accccggcaa
     4981 aatcgaagtg gcggaagtct tcgcctattc ctgcccccac tgcttccatt tcgagcccct
     5041 gctgcaggcc tgggaaaaga aacaggcggc cgacgtttac ctgatgcagg tgcatgccaa
     5101 ctggaactcg cagatggaac cgctgatccg gggttactac acgtcggtgg cgctcaaaat
     5161 cagggaccaa acccacatgg ccgtgttcaa cgcactgcat ctggagcaca agaacctgac
     5221 atcggccgag cagtgggccg acttctttgc ccccttcggc atcgacaagg ccaaagccat
     5281 cagcacctac aactccttcg gcgtggccag ccaggtcaaa caggccgatg cccgcacgcg
     5341 ccagtaccag gtgcggggaa cgccggagct gatagtgaat ggcaaatacc gcgtcagttc
     5401 acgcctgacc ggtggccagg aggagatgct gaaggtggcg gattttctgg tggccaaaga
     5461 gcgcgccgca cgccagggca gccagtagcc agccccggtt gcggcccccg ccccgctgca
     5521 cttgactccc agcaacagcc ggcagcaggc acagggtcaa ttctttgagc ggcacatccc
     5581 ctagtctggt cccatccgtt ctctttgggg cagtaacgga acgggccaag ctaaccactt
     5641 ccaaggggat caaccatgaa gacaaaccac cacgggttac agggcctgcg cacactcgca
     5701 gccctgtgcc tgaccttact actcaccgcc tgcggcggct ccgacatcca gttccagacg
     5761 ccggccaaga acgcagtata cggctcggcg gatctcattg atttcgacat ccgtttcgtc
     5821 aaaaccctgc ccgccgacgc caccttcaaa ctgaacggca ccgacgtcac ctaccagttc
     5881 gccgtggaca acaccgccgg caccgccacc gcgcagctgg cggcctccac cattctggat
     5941 gaaaagaacc ggctggaagt cagcacgccg caggtcaccc gcaccctggt gttctatgtg
     6001 gatcgcaccg ggccggaatt tcacattacg aatattgatc cctggctgat gctgcccggt
     6061 gtcaccgtga cgttttccgg ctatgtcacc gacctgtcac ccatcaacac cagtggcgcg
     6121 cccggcaccc tgccaccggc actctgggtt aagaccggcg acgccagtta ccgcagcgtg
     6181 gcgccgatca ccctgaatga aaacaacgaa ttctcggtga cctaccagct ggaagccgat
     6241 cccagcggcg tggtgggtga attcctgccg ctggtgttcc gggccgagga tattcatcgc
     6301 atccgcagca acgacgcctt tggctccatc gtcaacgtgc cgcaactggc caaggccaag
     6361 gtcacctcga acgcgtttgc caacgtgatt acaccggccg ccgaaaccgc cgtgaatgca
     6421 ctgaatctgg aacagctgat caaggcgcgc aacccggtgg tggacaaggg ctggtccatt
     6481 ttttccttca agctggatgt gaccaatttt gaactggacg atctggatct cgccatcctg
     6541 cccaccagcg aaggcggcta tcaggtgcgc gccaacgtca acgcacaaaa cctggatgta
     6601 agtatctgga gccgcgtgct ggccgacgtg tacctgggca ccatcggcgt gggcgtgcct
     6661 accggcctgc acaatgcgga cgtgaccggc aacgtactgg tgaaattccg cgtcgacggc
     6721 aatcccaacg ccaacccgac gctggtggtg gatcacatcg agccgaacct gaatatcatc
     6781 ggcggtgatt tcgacgccat cgatttcggt ttccccatcg atttgcccgg ccttggcacg
     6841 ctggaacaac tgatttttga agccatcgac agctctatcg ccgaactgat cggggaaaag
     6901 gtgggtgaaa aagtcgctga caaaatcaac gaccagctca aactgattcc cacggatttt
     6961 gatatcacgc tgcgcaacaa gacgctgaat ttctacctga tgacggcgtc cgtcactggc
     7021 gacagcaccg gtctggaagt ggcggtgacc gaagccgatg ccctgccacc ggtcaaccag
     7081 cgcgacccca gcgttattcg cgagccgggc tatcacattc acgatcgcag cgccgtgctg
     7141 ccgggctttt ccgccaccac tcccaatggc cagccgttcg atgtgggcct gagcctggac
     7201 tgggacattc tcaacaagac catctacagc gcccacctca ccggcatcga caaactgtcc
     7261 accaccgtgg ccggcagctc gattccgaaa ctgggcacca tcctgaaaaa ttatgagatg
     7321 aaactggatg tggtgccgct gtattcaccg tatctggatc tgccgctgcc gcgcactacg
     7381 cccgcactgg ccagcgtgca ggcgaaagat gtgcaaatca ccgcacaggt acgccgcacc
     7441 gatatcgagg acacgttcca cccgctgatc cagatcgtcg ccaatgtgcg ggcggacatt
     7501 ggcctgggcc tgagcggcaa ccgcatccgc gtgctgctgg acaccgatcc ccgcatcgat
     7561 atcgattcag tggtgaagga gcaatcctcg atcgtgctgg atgaacagtt cattcaggcg
     7621 ctggtggatt ttgcgattcc caaggtcatg ccgcgactgg cagatgcaat tcaggaaatc
     7681 cgcctgccga cagtctcggg ctacacgctg aatctgctgg aagccgaaaa catcggcaac
     7741 cggttcttca aggtgtatgc gcgggtgcag caggggaaca cgactggcgg tggcggtggc
     7801 atctgctgca tcgggggagt gggtggtatt ggcggtgtgc tggaagccga actggctacg
     7861 cagtgagagc ccgaccttaa cgtccagatt ccgaaagtca acaaggccac atgccgcccg
     7921 cttcaatcgg gcggcatgtt tttttggatc atgggctggc agggagaacg gctttgggcg
     7981 agtggctctt gttatgtacg attgctccac gaacctggat cctcccgaac gcgaaaaaca
     8041 gcagtgacga taattttatt ttgttgaaca agacaataaa tgcagcgagg aaaacggcga
     8101 attggtttgc gcaaggtggg ttcaatagcg catgacgtaa acacaagtcc agaccgcacg
     8161 cttgtccaat ccgaacactc atcattatct atttcaaaac ctgcaaggcg cctatccagc
     8221 tccttcgctt gctcatcggt aacgtcaaat tctgaaccct gatcttccaa gctatcccaa
     8281 agcgcttcag ccaacattat tcgctcaggc atggtaatct cttgaatctt cataaaaaca
     8341 acccaaacaa gtcccatagg tttggctggc ttaccacagc ttgaaactga ggcagttcag
     8401 aacacaccac gttctgcggc aaatttagag tgagcttggg aatttgttcg acagcggtga
     8461 tttgttagct tcttgttctc gcccttgcaa caacatgtct gggcgaaaaa ttttgcagcg
     8521 agattaatac cgtttcgggc tgaaaacctg ccatttttac aaaccctttg cttcaactat
     8581 cggccaggag cgaacattat tatatctgcc tgcaatgggc taaactcagg cctggac
//
LOCUS       DASUEG010000152         4817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_912690, whole genome shotgun sequence.
ACCESSION   DASUEG010000152 DASUEG010000000
VERSION     DASUEG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912690"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="VFX11_02865"
     CDS             <1..1730
                     /locus_tag="VFX11_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4937586.1"
                     /translation="DVLEAHRQWQQLPDFSAQLDRDTADAMLAEAARITEERIAPLNR
                     SGDEEGARWDSGVVTTPAGFVDAYRVWRDGGFTALTGNPDFGGLGMPKALSVLVEEML
                     YAANSSFALYPALSIGACMSIDAHATNELKQRFLPKLYAGEWTGSMCLTESHSGSDLG
                     LIRTKAVPNGDGTYAISGTKIFITGGEHDLSDNIVHLVLAKLPDAPAGSRGISLFLVP
                     KIWVNEDGSLGHHNGVSCGSIEHKMGIKASATCVLNFDGARGYLVGEANKGLACMFTM
                     MNCERLSIGIQGTGCAEMSYQNAVAYARERLQGRDVNGGDKSRAADPIIVHPDVRRML
                     LNIRSTTEGGRALAVYLGMQLDLANHADSAEQRARADALVALLTPVAKAFFTDLGLDG
                     TVLGQQVFGGHGYIREWGQEQLVRDARIAQIYEGTNGIQALDLIQRKVFANRGSSIDL
                     FLAEVNDCAKQIGSATLKPFAADVLQRSAQLQDLTHTLLDRGAAAPAELGAAACDYLH
                     VVGYVTYAYLWLRMMAVAEQQKGNPFALTKLKTGTYYFHRILPRVDSLIAAIRAGASD
                     IMAFSAGEF"
     gene            complement(1803..3689)
                     /locus_tag="VFX11_02870"
     CDS             complement(1803..3689)
                     /locus_tag="VFX11_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937587.1"
                     /translation="MKHSAQLKLCSTLFAAALGVVTAASSRADSFDDAVTRYLKGFEH
                     CKEAKAHLGAGRVDAAQSSLKKYDKLLDEAAGIHRDILTTNKRGMDGNLKYCQRVARD
                     VEVEAGMPVMNQALAACDNAQQALQENKPDVAKSRLEQFRSLKAEALAIAPSLSDIFT
                     VKNQISRCERIESKIAASSKQQVALALALETAQEESTAFVTSCQKTLEQLNREKIDDP
                     VLRDARQGQTSAASHRKNAQGEKLAQATFREQPNHPVKLAVDANLKQGDQCMTSLNQT
                     IARKDSELAAIKTRFDTVGQQLQTASAHCEQARQAADRTPTKDLQETVRSGYEKALQT
                     RNQVRDSLGKDAAYREYDGWGSVRGIEQGMQTLNQCLDNTRQQLDRLYAALPAAVTTA
                     AVATPAVAPDSAAAVAGKAGTSTAPAITTASTTPATKSTTAGQTDQLSGAAASRITGT
                     LRLLNLTPEFALFYVPDGTRPRPAEIVIDRAGFESPLYVCASGDNLTLRNRDNTSHRV
                     TASNESLSMSSVLARLQPRQSKTASVNWPEHQIVTLRADRGALAISYVANVPSANYQQ
                     LRLESAGKDTRFSFSNDKGVKTAYLIMPDTDPLTFTLTKGETKSLALTRNGVPAGSLL
                     VTGE"
     gene            complement(3807..>4817)
                     /locus_tag="VFX11_02875"
     CDS             complement(3807..>4817)
                     /locus_tag="VFX11_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX4937588.1"
                     /translation="KAQLQANDRSWLESSLTYPEDSVGDIMSRDSLIVADDISVNAAI
                     EQVRSADELPSQTDKLFVVDRHKHLVGVVPLIALMRHDGERPLNAIMDRDLVSFFPED
                     DAEEAGQAFERYDLISAPVIDDRRRVVGRITVEAVMDYLRARAENLALAKEGLSADTD
                     LLGPVWQGARERWPWLCINLMTAFLATRFISLFEGTIQQLVALATLMPIVASVGGNTG
                     NQTSALLIRGLALDQVHRDTLSFIYRKEISIGLINGLLWGSVLGLFAWGLYHNWLLGL
                     VMMGAVTLNLMLAALIGISVPLLLDRFDRDPAMGSSVVLTFATDSMGFFIFLGLATLT
                     LT"
BASE COUNT          800 a         1441 c         1587 g          989 t
ORIGIN      
        1 acgacgtgct ggaggcccat cggcaatggc agcagctacc ggacttctcc gcccagcttg
       61 accgcgacac cgccgacgcc atgctggcag aagcggcccg catcaccgag gaacgcattg
      121 ccccgctgaa ccgctccggc gatgaggaag gcgcacggtg ggacagcggc gtggtcacca
      181 cccccgccgg cttcgtggat gcttaccggg tctggcggga tggcggcttc actgccctga
      241 ccggcaaccc ggacttcggc ggcctgggca tgcccaaggc cctgtcggtg ctggtggagg
      301 aaatgctcta cgccgccaac agttcgttcg cgctgtatcc cgcattgtcc atcggcgcct
      361 gcatgtccat tgacgcccac gccacgaacg aactcaagca gcgcttcctg ccgaaactct
      421 atgccggcga atggaccggc tccatgtgcc tgacggaatc ccattccggc agcgacttgg
      481 gcctgatccg cacgaaagcc gtgcccaacg gcgacggtac ctacgccatc agcggcacca
      541 agattttcat caccggtggc gagcatgacc tgagcgacaa catcgtccac ctggtgctgg
      601 caaaactgcc cgacgcgccg gccggttccc gcggcatttc cctgttcctg gtaccgaaaa
      661 tctgggtaaa cgaggatgga tcactgggac atcacaatgg cgtcagctgc ggttccattg
      721 aacacaagat ggggatcaag gcgtccgcca cctgcgtgct gaattttgat ggcgcgcgcg
      781 gttatctggt gggcgaggcg aacaagggac tggcctgcat gttcaccatg atgaactgcg
      841 agcggctgtc gatcgggatt cagggtactg gctgcgccga gatgtcgtac cagaacgcgg
      901 tggcctatgc ccgcgaacgc ctgcaggggc gcgatgtgaa tggcggcgac aaatcccgcg
      961 cggcagatcc gatcatcgtg catccggacg tgcgccgcat gctgctgaac attcgctcca
     1021 ccaccgaggg cggtcgcgcg ctggcggtgt atctgggcat gcagctggat ctggccaatc
     1081 atgccgacag tgccgaacag cgtgcacgcg ccgatgcgct ggtggcgctg ctgacgccgg
     1141 tggcgaaggc cttcttcacg gatctgggtc tggatggcac ggtgctgggt cagcaggtgt
     1201 tcggcggcca cggctatatc cgtgagtggg gccaggagca actggtgcgt gatgcgcgca
     1261 tcgcccagat ttacgagggc accaacggca ttcaggcgtt ggacctgatt cagcgcaagg
     1321 tgttcgccaa tcgcggcagc agcatcgatc tgtttctggc ggaggtgaat gactgcgcga
     1381 aacagattgg cagtgccacc ttgaaaccgt ttgcggcgga tgtgctgcag cggtcggcgc
     1441 agttgcagga tctgacccac accttgctgg atcgcggtgc tgcggcgccg gcggaactcg
     1501 gggcggctgc ctgcgattac ctgcatgtgg tggggtatgt cacctatgcg tatctgtggc
     1561 tgcgcatgat ggcggtggcg gagcagcaga agggcaatcc gttcgcgctc accaagctca
     1621 agaccggcac ttactacttc catcgtatcc tgccccgggt ggacagcctg attgcagcca
     1681 tccgcgccgg tgccagcgac atcatggcct tttccgccgg agaattttga ggcgctctgc
     1741 cgcaggaggc ttttccagac aagaagggtg tgcggcataa ccggcacacc cttttctgtt
     1801 gcctactccc ctgttaccag caggctgccg gccggcacgc cgttgcgggt gagcgccaga
     1861 cttttcgtct cgcccttggt gagggtaaag gtcagcgggt cggtgtccgg catgatcaga
     1921 taggcagttt tcacgccctt gtcgttgctg aagctgaagc gggtgtcctt gccggcggat
     1981 tccaggcgaa gctgctggta attggcgctg ggcacattgg ccacgtaact gatggcgagt
     2041 gcgccgcgat cggcacgcag ggtgacgatc tggtgttccg gccagttcac gctggcggtc
     2101 ttgctctgac gcggctgcag gcgggccagc acgctggaca tcgacaggct ttcattgctg
     2161 gcggtgacgc ggtggctggt gttgtcccgg ttgcgcaggg tcaggttgtc gccgctggcg
     2221 cagacgtaca gcggggattc gaatccggcc cggtcgatga cgatttccgc cggccgcgga
     2281 cgggtgccgt ccggtacgta aaacagcgcg aactcaggcg tcaggttcag caggcgcagt
     2341 gtgccggtga tgcggctggc agcggcgccg ctcagttggt ctgtctgtcc ggctgtggtg
     2401 gattttgtcg ctggcgttgt tgaggccgtt gtgattgcgg gtgcagttga ggtgccggcc
     2461 ttgcctgcca ccgcagccgc agaatcgggc gcaacggcgg gcgtggcaac ggctgccgtc
     2521 gtgaccgctg ccggcagcgc cgcatacagg cggtcgagtt gctggcgggt gttgtccagg
     2581 cactgattca gcgtctgcat gccttgctca atgccgcgta cgctgcccca gccgtcatat
     2641 tcgcgatagg ctgcatcttt ccccagactg tctcgtacct gattacgggt ttgcagggct
     2701 ttttcgtagc cgctgcgcac tgtttcttgc aggtcttttg ttggcgtccg gtccgccgcc
     2761 tgtcgtgcct gctcgcaatg ggcggaggct gtctgcaact gctgtcccac cgtgtcgaag
     2821 cgggtcttga tggcggccag ttcgctgtcc ttgcgggcaa tggtctgatt caggcttgtc
     2881 atgcattgat cgccctgttt cagattcgcg tccaccgcca gtttcaccgg gtggttgggc
     2941 tgttcgcgga atgtcgcctg ggccagtttc tcgccctgtg cattcttgcg gtgggaagcc
     3001 gcgctggtct ggccttgccg ggcgtcccgc agtaccgggt cgtcgatctt ttcccggtta
     3061 agctgctcca gggttttctg gcagctggtg acgaaggcgg tgctttcctc ctgtgccgtc
     3121 tccagcgcca gcgcgagggc cacttgctgc ttgctggatg cggcaatctt gctttcgatc
     3181 cgttcgcagc ggctgatctg gtttttgacg gtgaagatgt cgctcaggct gggggcaatg
     3241 gccagcgctt cggctttcag gctgcggaac tgctccagcc gggatttcgc aacgtccggc
     3301 ttgttttcct gcagagcctg ctgggcattg tcgcaggcgg cgagagcctg gttcatcacc
     3361 ggcatgccgg cttccacctc aacgtcgcgc gccacccgct ggcaatattt cagattgccg
     3421 tccatgccgc gcttgttggt agtgagaatg tcccgatgga tgccggcggc ttcatccagc
     3481 agcttgtcgt actttttcag gctgctttgg gcggcatcta cccggcctgc gcccaggtgt
     3541 gccttggctt ccttgcagtg ttcgaagcct ttcaggtacc gggtgacggc gtcatcgaag
     3601 ctgtcggcgc ggctgctggc ggcggtgacg acgcccaagg ctgcggcgaa aagggtggag
     3661 cagagtttta actgcgcaga atgtttcatc cggggcctgt cttgtccagg tggaagcggg
     3721 tcacgggcgc tctcggtttc gcccgtttca tccgattaag attagaccag tcgggatgaa
     3781 ggcagggtga ttttgaaacc agtcagtcac gttagcgtga gagtggccag ccccagaaag
     3841 ataaagaagc ccatgctgtc ggtcgcgaag gtgagtacca ccgaagaccc catggcggga
     3901 tcgcggtcga agcggtccag cagcagtggc accgagatgc cgatcagggc cgccagcatc
     3961 aggttcaggg tcactgcccc catcatcacc agccccagca gccagttgtg atacaggccc
     4021 caggcgaaca gccccagcac cgagccccac agcaacccgt tgatcaggcc gatggagatt
     4081 tccttgcgat agatgaagga cagggtgtcg cggtggacct ggtccagtgc cagaccgcgg
     4141 atcagcaggg ctgaggtctg attgccggta ttgccgccca cgctggccac gatgggcatc
     4201 agcgtcgcca gtgccaccag ctgctggatg gtgccctcga acaggctgat gaagcgggtg
     4261 gcaagaaaag ccgtcatcag gttgatgcag agccagggcc agcgctcgcg ggcaccctgc
     4321 cagaccgggc ccagcaggtc agtatcagca ctcagacctt ccttggccag cgccaggttc
     4381 tcggcccggg cgcgcagata gtccatcacc gcttctacgg tgatgcgccc taccacccgg
     4441 cggcgatcgt cgatcaccgg ggcggaaatc aggtcgtagc gttcgaacgc ctgacccgct
     4501 tcctcggcat cgtcttcagg gaaaaagctc accagatcgc ggtccatgat ggcgttgagc
     4561 ggacgttcgc cgtcatggcg catcagggcg atcaggggaa cgacaccgac gagatgcttg
     4621 tgacggtcga ccacgaacag cttgtcggtc tgggacggca actcgtcggc ggagcgcacc
     4681 tgctcgatgg cggcgttgac gctgatgtca tcggccacaa tgaggctgtc gcggctcatg
     4741 atgtcgccga cgctgtcttc gggataggta aggctgcttt ccagccagct gcgatcgttg
     4801 gcctgcaact gggcctt
//
LOCUS       DASUEG010000153         4335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2017173, whole genome shotgun sequence.
ACCESSION   DASUEG010000153 DASUEG010000000
VERSION     DASUEG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017173"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="tolA"
                     /locus_tag="VFX11_02880"
     CDS             <1..523
                     /gene="tolA"
                     /locus_tag="VFX11_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX4937589.1"
                     /translation="PKKPEVKKPEPKKPEPKKPEPKKTPPKKVEPTEDFSDILKSEMK
                     QLEKQKPKAEAAKSAKPAAAAAAATATSDEEQDEIQQYIGLMQQTIARYWVRPPSART
                     GMVVTLRINLLPGGELNDVNVVESSGNPAFDRSAVVAVQKAGRFSVPDDPVKFDRYFR
                     TLKLQFNPEDLKY"
     gene            546..1829
                     /gene="tolB"
                     /locus_tag="VFX11_02885"
     CDS             546..1829
                     /gene="tolB"
                     /locus_tag="VFX11_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX4937590.1"
                     /translation="MFVRKLLALFLFCVASVANAELTVIINSGQDDAIPIGIVPFGWN
                     QPSRLPEDVAAIVHADLYRSGQFKPLERADMLSRPTEASQVFYRDWKVLGTEYLVIGR
                     MQPAGGGQYRADFEIFDVFQQRPIKKGTVTSNNLRGMAHYISDVIYEAITGIRGAFST
                     RMLYVTMERMQARKQRYRLYVADVDGFNEKIILESPEPILSPTWAPNGRHIAYVSFQS
                     TRPAIYIQDTITGRQQKITGFPGLNGAPSFSPDGRQLAMTLSKDGNPEIYVMDVASGS
                     LRRITNHYAIDTEATWSPDGQNLIFTSERGGSPQIYRQHIASGKVERLTFEGSYNARP
                     ALTPDGRFLAMVHRSNGVFHIAVKDLVKGTFNVLTETDLDESPSIAPNASMILYATLK
                     GGRGVLGAVSLDGRVKVQLPSRFGDVREPSWSPFL"
     gene            1908..2414
                     /gene="pal"
                     /locus_tag="VFX11_02890"
     CDS             1908..2414
                     /gene="pal"
                     /locus_tag="VFX11_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX4937591.1"
                     /translation="MPMFTRNHLIALIAAASIAGCSSTPEETTDTTTAAPVTSGSTTD
                     TTGGYGSSDPSLQNRVVYFDFDKYDIRVDSEATLNAHAQYLSAHPGAKLRVEGHADER
                     GTREYNMALGERRAKATSAFLTSKGASASQIEVISYGEERPVALGHEEGSWAQNRRAE
                     LQYTSEAP"
     gene            2414..3313
                     /locus_tag="VFX11_02895"
     CDS             2414..3313
                     /locus_tag="VFX11_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HEX4937592.1"
                     /translation="MQRLAAFCVLMAATTVSVVAAPPYEPVKRSSSASPIDPSSPYYS
                     PSQNEGAGAVYGSGVKTYGTGGTDGGDTSYAKPAESARPSTYSSASTAGFDSYSYMQQ
                     MQQMQQEIQELRGAVEQLTHDMEMMQKQARERYLDLDSRINQLRGGTAAAAGAAVAVD
                     QAGSPAAGSAVESTGEDKDLYDKASQLRRDGKYEESIAVLEDLLKRSPEGLYAPYCEY
                     WLGETYMALKPPKMDVAKTHFISLLGNHPDHVKVPDAMYKLGKLFASQGENSKAKSTL
                     NELIKKHPDKPAANLAKDLLKTL"
     gene            3398..4042
                     /gene="queE"
                     /locus_tag="VFX11_02900"
     CDS             3398..4042
                     /gene="queE"
                     /locus_tag="VFX11_02900"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX4937593.1"
                     /translation="MLQVRVSEIFHSLQGEARTVGWPTVFVRLTGCPLRCQYCDTAYA
                     FSGGELLAIDDILEKVGRHQSRYVTVTGGEPLAQPNCLPLLNRLCDLGYDVSLETSGA
                     LSIEGVDPRVSVVLDLKSPDSGECHRNLYDNIPLLKPIDQVKFVLCSRNDYQWARLKL
                     DEFNLAGRVQDVLFSPSYQQLSPTDLAEWILEDRLPVRFQIQLHKLLWADEPGR"
     gene            4061..>4335
                     /locus_tag="VFX11_02905"
     CDS             4061..>4335
                     /locus_tag="VFX11_02905"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018168454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX4937594.1"
                     /translation="MNTQVSKAVVLLSGGLDSATCLALARSQGYECHAISFEYGQRHE
                     SELRAAEQVAKAQGAAAFKVIHINMGDIGGSALTDRSIAVPESPTDG"
BASE COUNT          944 a         1227 c         1232 g          932 t
ORIGIN      
        1 gcccaaaaag cctgaggtga agaagcccga accgaaaaag ccggagccca agaagcctga
       61 gcccaaaaaa acgcccccga aaaaagtgga gccgaccgag gacttcagcg acatcctcaa
      121 gagcgaaatg aagcagctgg agaagcagaa acccaaggcc gaagctgcca aatcggccaa
      181 gccggcggcg gcagctgcag cggcgacggc caccagtgac gaggagcagg acgagatcca
      241 gcagtatatc ggtctgatgc aacagacgat tgcccgttac tgggtgcgcc cgcccagcgc
      301 ccgtacggga atggtggtaa ccttgcgcat caatttgctg cccgggggcg agctaaatga
      361 cgtgaacgtg gtcgaaagca gcggtaaccc cgcttttgac cggtctgcag tcgtggcggt
      421 gcagaaagct ggacgtttca gcgtgccgga tgatccggtc aaattcgacc gctatttccg
      481 taccctgaag ctgcagttca atcctgaaga cctgaaatac tgattcaccg gagacgttag
      541 ttgccatgtt tgttcgtaaa ctgttagccc tgttcctgtt ctgtgttgcc tcggtggcaa
      601 acgctgagct gacggtcatc atcaactcgg gccaggatga cgccatcccc atcggcatcg
      661 ttccctttgg ctggaaccag ccatcccgcc tgccggagga cgtcgccgcc atcgtgcatg
      721 cagatctgta ccgctcgggc cagttcaagc cgctggagcg ggctgatatg ctcagccgtc
      781 cgacggaagc cagccaggtg ttctatcgcg actggaaggt tctgggaacc gaatacctgg
      841 ttatagggcg catgcagcct gccgggggag gccagtaccg cgctgatttc gagatttttg
      901 acgtattcca gcagcgcccc atcaaaaaag gcaccgtcac cagcaacaac ctgcgtggca
      961 tggcccacta catcagcgac gtgatctacg aagccattac cggcattcgc ggcgcgttct
     1021 ccacccgaat gctctacgtg acgatggagc gcatgcaggc ccgcaagcag cgctaccgtc
     1081 tctacgtagc ggatgtggat ggcttcaatg agaagatcat tcttgagtcc cctgagccga
     1141 tcctgtcgcc aacctgggct cccaacggtc gtcatatcgc ctacgtgtct ttccagagca
     1201 cccgtcctgc catttatatc caggacacga tcacaggtcg acagcagaaa atcacgggtt
     1261 ttccaggcct gaacggagcg ccctcgttct cgccggacgg tcgtcagctg gccatgacac
     1321 tgtcgaagga cggcaatcct gaaatctatg tcatggacgt ggcgagcggc agtctgcgtc
     1381 gcatcaccaa ccattacgcc atcgacacgg aggccacctg gtcccctgac ggacaaaacc
     1441 tgattttcac gtcggaacgc ggcggatctc cacaaattta ccggcagcat attgcatcag
     1501 gaaaggtgga acgattaaca ttcgagggca gttacaatgc gcgtccagca ttgactccag
     1561 atggtcgttt cctggccatg gtgcatcgct ctaacggggt gttccatatt gcggtcaagg
     1621 atctggtcaa gggcacgttc aatgtcctga ccgaaacaga tctggatgaa tcgcccagta
     1681 tcgctcccaa tgccagtatg attctgtacg ccacgctcaa gggcggtcgt ggcgttttgg
     1741 gggctgtatc tctggatggg cgagttaaag tgcagctacc atcgcgcttt ggagatgtgc
     1801 gggagccaag ctggtcgccg tttttgtaaa aaatttgttg cagcctgttt taaaagcttt
     1861 aatgaaccct taatattcgc gacgagtcca tctcttagga gtttgctatg cctatgttta
     1921 cccgtaatca tcttatcgcc ctgatcgctg ctgcatccat cgctggctgt agctccactc
     1981 cggaagaaac taccgacacc actactgctg ctccggtaac ttctggctct actaccgata
     2041 ctactggtgg ttacggttct tccgatccgt ccctgcagaa ccgtgttgtg tatttcgatt
     2101 tcgacaagta cgacatccgc gttgattccg aagccactct gaacgctcac gcccagtacc
     2161 tgtccgccca cccgggtgcc aagctgcgcg ttgaaggtca cgctgacgag cgcggcacac
     2221 gcgaatacaa catggctctg ggcgagcgtc gtgcgaaagc cacttccgct ttcctgacct
     2281 ccaaaggcgc ttctgccagc cagatcgaag tgatcagcta cggcgaagag cgtccggttg
     2341 ctctgggtca tgaagaaggt tcctgggctc aaaaccgtcg cgcagaactg cagtacactt
     2401 ctgaagctcc ctgatgcagc gattggcagc cttttgtgtg ctgatggcag ctaccaccgt
     2461 ttcggtggta gctgctcccc cctacgaacc cgtcaaacgc agttcctccg ctagccccat
     2521 cgatccttcc agcccttatt attctccttc ccagaacgaa ggtgcgggcg ctgtgtatgg
     2581 ttctggtgta aaaacctacg gtaccggtgg cactgatggc ggcgatacct cgtacgccaa
     2641 accggcggaa tccgctcgtc cttccactta ttccagtgca tccactgccg gtttcgacag
     2701 ctattcctac atgcagcaga tgcagcagat gcagcaggaa atccaggaat tgcgtggtgc
     2761 ggtggagcag ctcacccatg acatggaaat gatgcagaag caggccaggg aacgttatct
     2821 ggacctcgat tcccgcatca accagttacg cggtggcacg gctgcagcgg cgggtgccgc
     2881 agtggctgta gatcaggccg gctctccagc ggcaggctcg gcagttgagt ccaccggtga
     2941 ggataaagat ctctacgaca aggccagtca gttgcgccgg gacggcaaat atgaagagtc
     3001 cattgccgtg cttgaggatc tgctgaagcg ttcacccgaa ggtctttatg ctccttattg
     3061 cgagtattgg ctgggtgaaa cctatatggc gctcaagccg cccaagatgg atgttgcgaa
     3121 aacccacttc atcagtctgc ttggcaatca ccccgatcat gtcaaggtac cggatgccat
     3181 gtacaagctg ggcaagctgt ttgcgtccca gggtgagaac tccaaggcta aaagtaccct
     3241 gaatgagctg atcaagaagc acccggacaa accggcggcg aacctggcca aggatctgct
     3301 caaaaccctt tgaacctgtg cccgcgcggc cattctgttg agttagcaag cgggcttcag
     3361 gtaccatgtg ccccctttct gcagacaggt gtgagtcgtg ctccaggtac gcgtcagcga
     3421 gattttccat tccctgcaag gtgaagccag gacagtagga tggcctacgg tgttcgtgcg
     3481 gctgaccggc tgtcctctgc gatgccagta ttgcgatacc gcctacgcgt tctccggcgg
     3541 tgagctgctt gccattgacg acattctcga gaaagttggt cgtcatcaat cccgatacgt
     3601 gaccgtgacc ggtggtgagc cactggccca gcccaattgc ttgccgctgt tgaatcgcct
     3661 gtgtgatctt ggttatgacg tttcattgga aaccagcggg gcgttgagta ttgagggtgt
     3721 cgatccgcgc gtcagtgttg tgctggacct gaaaagcccg gattccggcg agtgtcatcg
     3781 caatctttac gacaacattc cgctgttgaa gcccatcgat caggtgaaat tcgttctttg
     3841 cagccgcaat gattaccagt gggcgcggtt aaagctggat gagttcaatc tggctggtcg
     3901 cgtgcaggat gtactgttct ctcccagtta ccagcaactg tcgcctaccg acctggcgga
     3961 gtggattctg gaagaccggt taccggtacg gttccagatt cagctgcaca agctgttgtg
     4021 ggcggacgag cccgggcgct gatgccagga tgatgacgcg atgaatacgc aggtgagcaa
     4081 agccgtggtg ctgttgtcag gtgggctgga ttcggccaca tgcctcgcgc ttgccagaag
     4141 ccagggttac gagtgccatg ccatcagctt cgaatacggt cagcgccacg agtcggagct
     4201 gcgcgcggca gagcaggtgg cgaaggcgca gggggcagcg gcattcaagg tcatacacat
     4261 caatatgggt gacattggcg gatcggcgct gacagacagg tccattgcgg tgccggaaag
     4321 cccaactgac ggcat
//
LOCUS       DASUEG010000154         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_414580, whole genome shotgun sequence.
ACCESSION   DASUEG010000154 DASUEG010000000
VERSION     DASUEG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414580"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFX11_02910"
     CDS             <1..888
                     /locus_tag="VFX11_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4937595.1"
                     /translation="KENSEPFVRVARHLLRAGVQVPELLALDLAQGFMMLSDFGDRLL
                     LPALTADSVDGHYGTAMRTLIDIMQADFSDAPLPAYNQPLLRREMELFREWFCGVHLQ
                     LSLSADENRQLDDVFTWLEQQALAQPQVTVHRDYHSRNLMLLDDGGLGVLDFQDAVHG
                     PITYDLVSLLRDCYISWPEDQVKEWTLRFADRLRRVSLLDDVTDATYWQWFNGMGAQR
                     HLKVAGIFARLNHRDGKPGYLNDIPLTLAYLIQECESFQGNGRHLMAPFADWLRQRIV
                     PALLAKQPAAANLLGRYAP"
     gene            885..1574
                     /locus_tag="VFX11_02915"
     CDS             885..1574
                     /locus_tag="VFX11_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4937596.1"
                     /translation="MMAMLLAAGRGERMAPLTDHTPKPLLPVAGKPLLQHHIERVAAA
                     GFTGLVINTAWLGQQIADFVGDGSRFGIPVRVSAEGERLETGGGIFNALPLLGSAPFA
                     VINSDIWTDFPLASLPRQISGQAHLVLVPNPDHNPRGDFALEKGQCVPGGDNRLTFSG
                     ISVLTPELFAGCQPGRFPLAPLLRDAIARGKVSGQRHDGIWIDVGTPQRLQAVETLLQ
                     HPGSSFKEYDV"
     gene            1571..2401
                     /gene="djlA"
                     /locus_tag="VFX11_02920"
     CDS             1571..2401
                     /gene="djlA"
                     /locus_tag="VFX11_02920"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEX4937597.1"
                     /translation="MTSFIWLGPLIGVLTGFFLAGGGVVGVVGATLGAWLGFQFDTII
                     QLSSSVYQFTTRGKGGDTEIQQAFFRATFICLGKVAKCDGAVCDAEIQWTTAVMDRMG
                     LSAEKKREAIELFDLGKSGQHDLTAELTRLHETCGRRVTLLQIFMEIQVQAALSDGAL
                     DQKEWATLSHIAQTIRFRITLLERIVRSAQAYQEFRTQDKSRPRSPQEELLRAYEILG
                     IEPTASVAELKRAYRRLMSQHHPDKLIARGLPEEMLDMAKEKTQAIRTAYEAIMASRK
                     "
     gene            complement(2431..2844)
                     /locus_tag="VFX11_02925"
     CDS             complement(2431..2844)
                     /locus_tag="VFX11_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014999075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX4937598.1"
                     /translation="MLEQLQYSLTGLQSFVPYFFMALTDLILFKLAYTALTPHDEWKL
                     VKQSNSAAAIALGGAIVGYAIVLEEAINSSINLVDFAVWSAVGFAIQLLAFFITRLFV
                     PQLSQRITEGQVAAGTMSAAFSIAIGLLNAACISF"
     gene            complement(2985..>3389)
                     /locus_tag="VFX11_02930"
     CDS             complement(2985..>3389)
                     /locus_tag="VFX11_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937599.1"
                     /translation="ALNGTPKGRMLAIRGVDKTFLFSPITAFSKLSLFPWDGKSFNAT
                     SDTTGDGINRINLLAQLNWFPFKTRIEPSVIDGKDCIYLDYEQPGNPFFIAKIRDELR
                     EVAPGLFLGPAMWKTGKGSAELVLWFAIDTTR"
BASE COUNT          643 a          985 c         1093 g          668 t
ORIGIN      
        1 aaggaaaaca gcgagccgtt cgtgcgggtg gcgcggcatc tgctcagggc cggcgtgcag
       61 gtgccggagc tgctggcgct ggatctggcg cagggtttca tgatgctgag tgatttcggc
      121 gatcgcctgt tgttgccggc actcaccgcc gattcggtgg acggtcacta tggcaccgcc
      181 atgcgcacgc tgatcgacat catgcaggca gatttttccg acgcgccgct gccggcctac
      241 aatcagccac tgctgcggcg ggaaatggag ctgttccggg agtggttttg cggcgttcat
      301 ctgcagttgt cgttaagcgc cgatgaaaac cgccagctcg acgacgtgtt cacctggctg
      361 gaacagcagg cgctggcaca accccaggtc accgtgcacc gggattacca ttcgcgcaac
      421 ctgatgctgc tggatgatgg cggcctgggc gtgctggact tccaggatgc cgtgcacggg
      481 cccatcacct acgatctggt gtccctgctg cgggattgct atataagttg gcccgaggat
      541 caggtgaagg agtggacgct gcgttttgcc gatcgcctgc gccgggtgag cctgctggat
      601 gacgtgactg acgccaccta ctggcagtgg ttcaacggca tgggcgccca gcggcacctg
      661 aaggtggccg gcatttttgc acgcctgaac catcgcgatg gcaagcccgg ttatctgaac
      721 gacattcccc tcacgctggc ctatctgatc caggagtgcg aaagctttca gggcaacgga
      781 cggcatctca tggcgccgtt cgcagactgg ttgcgccagc gcatagtgcc ggctttgctg
      841 gccaagcagc cggcggcggc aaacctgctg ggcagatacg cgccatgatg gccatgctgc
      901 tggcagcggg acggggcgag cgcatggcac ccctgaccga tcacactccc aaacctctgc
      961 tgccggtggc gggcaaaccc ctgctgcaac atcatatcga acgcgtggcg gcggcaggat
     1021 ttaccgggct ggtgatcaat actgcctggc tgggtcagca gattgcggat ttcgtcggcg
     1081 acggctcccg tttcggcatt ccggttcgcg tcagcgcgga aggagagcgg ctggaaaccg
     1141 gcggcggtat tttcaatgcg ctacccttgt tgggcagcgc gccatttgca gtgatcaaca
     1201 gcgatatctg gaccgatttt ccgctggcat cgctgccacg acagatatcg gggcaggcac
     1261 atctggtgct ggtgccgaat ccggatcaca atccgcgcgg cgatttcgcg ctggaaaaag
     1321 gtcagtgcgt gcctgggggc gacaatcggc tgaccttcag cggcatcagt gtgctgacgc
     1381 cggaattgtt cgccggctgc caaccgggcc gttttccgct ggcgccgctg ttgcgggatg
     1441 ccatcgcacg tggaaaggtg agcgggcaac gccatgacgg catctggatc gatgtgggca
     1501 ccccgcagcg gctacaggca gtcgaaacgt tattgcagca tccaggatct tccttcaagg
     1561 agtacgacgt ttgacctcat ttatctggct aggccccctg atcggcgtgc tgacgggatt
     1621 tttcctggcg ggcggtggcg tcgtgggcgt ggtgggcgcg acgctcggcg cctggctcgg
     1681 cttccagttc gacaccatca tccagctcag cagcagcgtt taccagttca ccacccgcgg
     1741 caaggggggc gacaccgaga tccagcaggc ttttttccgc gcgacattca tttgtctggg
     1801 gaaagtggcg aagtgcgacg gtgcggtgtg cgacgccgaa atccagtgga ctaccgccgt
     1861 gatggaccgc atggggctga gcgccgagaa aaaacgcgaa gccatcgagc tgttcgatct
     1921 gggcaagtcc ggccagcatg atctcaccgc tgaactgacc cggctgcatg aaacctgcgg
     1981 ccgccgcgtc accctgctgc agattttcat ggagattcag gtgcaggcgg ccctgagcga
     2041 cggcgcgctg gaccagaagg aatgggccac cctgtcgcac atcgcccaga ccattcgctt
     2101 ccgcattacg ctgctggagc gcatcgtgcg cagtgcgcag gcataccagg aattccgcac
     2161 ccaggacaaa tcccgcccgc gctcaccgca ggaggaattg ttgcgtgcct atgaaattct
     2221 cggcattgaa cctactgctt cagttgcgga actcaagcgc gcctaccgcc gcctgatgag
     2281 ccagcaccat ccggacaagc tgatcgccag aggcctgccg gaggagatgc tggatatggc
     2341 caaggaaaaa acccaggcca ttcgtactgc ctacgaagcg atcatggcca gccgcaagtg
     2401 aatgcgggcg cgccagaata gcgctttgtt tcagaacgat atgcaagctg cgttgagcag
     2461 gccgatggca atggaaaacg ccgccgacat ggtgcctgct gccacttgtc cttccgtgat
     2521 gcgctgcgac agttgcggca caaacaggcg ggtgatgaaa aacgccagca gctggatcgc
     2581 aaatcccacc gccgaccaca ccgcgaaatc caccagattg atcgaactgt tgatggcttc
     2641 ctccagcacg atggcatagc ccactatcgc accgcccagc gcaatggcgg cggccgagtt
     2701 gctctgtttc accagcttcc attcgtcgtg cggcgtcagc gccgtgtagg ccagcttgaa
     2761 caggatcagg tcggtcagtg ccatgaaaaa atagggaacg aaactttgca ggccggtgag
     2821 ggaatattgc agttgctcca gcatggtcgg gattccgtga ggtgagggtg ggcggaatta
     2881 tacgggaggc gacgccaacc tccacggtgg acgggcgccc gctggcaagc gcccgtccac
     2941 cggatcgttc tgcatgaagt tctgtgtgca gtgctatgag ctgttcagcg tgttgtgtcg
     3001 atcgcgaacc agagcaccag ttcggcgctg cccttgccgg ttttccacat ggcggggccg
     3061 aggaacaggc cgggtgccac ttcccgcagt tcgtcgcgga ttttcgcaat gaagaacggg
     3121 ttacccggct gttcgtaatc caggtagatg cagtccttgc cgtcgatcac ggacggttcg
     3181 atgcgggtct tgaacggaaa ccagttcagt tgcgccagca ggttgatgcg gttgatgccg
     3241 tcgccggtgg tgtcgctggt ggcgttgaag cttttgccgt cccagggaaa caggctgagt
     3301 ttgctgaagg cggtgatcgg actgaacagg aaggttttgt cgacgccgcg gatcgccagc
     3361 atgcgacctt tgggggtgcc gttcagggc
//
LOCUS       DASUEG010000155         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_280156, whole genome shotgun sequence.
ACCESSION   DASUEG010000155 DASUEG010000000
VERSION     DASUEG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280156"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFX11_02935"
     CDS             <1..536
                     /locus_tag="VFX11_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937600.1"
                     /translation="GVSDSLFIHHFGSKLMLWREAVDRMIALEFPQLIARLSRLDHTA
                     SPLVLLRDNFAEILRLAQQRPAIFRLLFNELDSGGERADYLKEKYLIPYVSIFDHTIA
                     QCQAQGLIKPVSHSSLHALLLGAVNILIRPWILRQGKPADAQQAAISIPVEDLLEMMF
                     SGVVNPGAPGTAQNCER"
     gene            complement(518..2221)
                     /locus_tag="VFX11_02940"
     CDS             complement(518..2221)
                     /locus_tag="VFX11_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4937601.1"
                     /translation="MADVRHQDCQQYAQQCAKGRRASGAVVMAVVAGLLAPPATAALV
                     ENLTMGNAKALALGNAVTADPPGIDSIHFNPAGLARLAGRQYELKVLAAAMSFTVEFG
                     EHDAKSQQMIDAFGYEDDVVGQTSETSTIGLRLPYQEGIYEWPAPMLVVPLGGASYRP
                     PNSNITFATAVYAPMAAGYIRDEDDPARFMGQYLSLAKITYFSPSVGIEINDTLSVGA
                     AIHFSWQGVTAGTRIRVPNFALAFGEQLTKQLQDQNLCPNPGDPEPPVNLCGIDQEIA
                     RLGPYTDAASLEFDAETGMVTGVNLGVLWQPVSWFTWGAVYQFESSADLDGTYRLGYS
                     DEWVNFFTGLSQSDMGRALNVLIPMPTGQADSPYGRGLEQGDAVINVITPAHFATGIS
                     LQVTEQWKFNMDAKWTDWGAWDGLTVKFDQALDFTKLASIVSEYSELTELTIPRHYAS
                     VWNWAFGAEYQYNQRLALRFGYEPRKSSIPDDKQDVLLPLGDADLYTGGFEYKMGEGQ
                     LLAVGVGYLVASADVPANSSTNANSTDDYHNFIYNPYAGTAFESSAEAFLLELSYRSQ
                     F"
     gene            complement(2491..>2696)
                     /locus_tag="VFX11_02945"
     CDS             complement(2491..>2696)
                     /locus_tag="VFX11_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX4937602.1"
                     /translation="GTCRMGSDAATSVCTPHGETHDVKNLFITDASLFPTSIIVNPQV
                     TVYTLASYIADHILQNQAQYFAT"
BASE COUNT          530 a          817 c          822 g          527 t
ORIGIN      
        1 ccggcgtgag cgattccctg ttcatccatc atttcggctc gaaactgatg ctctggcggg
       61 aagcggtgga ccggatgatc gcactggaat ttccccagct gatcgcgcgc ctgagccggc
      121 tcgaccacac cgccagcccc ctggtgctgc tgcgggacaa ttttgcggaa atcctgcgtc
      181 tggcccagca acgtcccgcc attttccggc tgctgttcaa cgagctggac agcggtggcg
      241 aacgggccga ttacctgaaa gagaaatacc tgattcccta cgtgtcgatc ttcgaccaca
      301 ccattgcgca gtgccaggcg cagggcctga tcaagccggt gtcccacagt tcgctgcacg
      361 cattgctgct gggcgcggtg aacatcctga tccggccctg gattctgcgt cagggcaagc
      421 ccgccgacgc ccagcaggcc gccatttcca ttccggtgga agacctgctg gaaatgatgt
      481 tcagcggcgt cgtcaatccg ggtgcgccag gcaccgctca gaactgcgag cggtaactca
      541 gctccagcag aaacgcctcg gcactggatt caaaagcagt gcccgcataa gggttgtaga
      601 tgaagttgtg gtagtcgtcg gtgctgttgg cattggtgct ggaattcgcc ggcacgtcgg
      661 cactggccac cagataaccc acgcccaccg ccagcaactg gccttccccc attttgtatt
      721 cgaaccctcc ggtgtacaga tcggcatcgc ccagcggcag cagcacgtcc tgcttgtcgt
      781 cggggatcga actcttgcgc ggctcgtaac cgaagcgcag cgccagacgc tggttgtact
      841 gatattccgc gccgaacgcc cagttccaca cgctggcata atggcgcgga atggtgagtt
      901 cggtgagttc ggaatattcc gacacaatgg acgccagctt ggtgaaatcc agcgcctgat
      961 cgaatttcac ggtcagtccg tcccaggcgc cccaatcggt ccacttggca tccatattga
     1021 atttccactg ctccgtcacc tgcaggctga tgccggtggc gaaatgggcc ggggtgatga
     1081 cattgatcac cgcgtcgccc tgctccaggc cgcgtccgta gggtgagtcg gcctggccgg
     1141 tgggcatcgg gatgagcacg ttgagggcgc ggcccatatc cgactggctc aatccggtaa
     1201 aaaaattcac ccattcgtcg ctatagccca ggcgataggt gccatccaga tcggcggagc
     1261 tttcgaactg gtacaccgca ccccaggtaa accagcttac cggttgccac agcacgccca
     1321 ggttgacgcc ggtcaccatg ccggtttccg catcgaactc cagactggcg gcatcggtgt
     1381 aggggcccag ccgcgcgatt tcctggtcga tcccgcacag gttcaccggc ggttcgggat
     1441 caccgggatt ggggcacaga ttctggtcct gcaactgctt cgtcagctgt tcaccgaacg
     1501 ccagcgcgaa attgggcacg cggatgcggg tgccggcggt gacaccctgc caggaaaaat
     1561 ggatcgctgc gcccaccgac agcgtgtcgt ttatctcgat gcccaccgac ggtgaaaaat
     1621 acgtgatctt cgccagtgac aggtactggc ccatgaagcg ggcaggatca tcctcatcgc
     1681 gaatataacc ggcggccatc ggcgcataaa cagcggtggc gaacgtgatg ttggaattgg
     1741 gcggccggta ggaagcgccg cccaaaggca ccaccagcat gggcgccggc cattcgtaga
     1801 tgccctcctg ataaggcagg cgcagaccga tggtggaggt ctcgctggtc tgccccacca
     1861 cgtcatcctc gtacccaaag gcatcgatca tctgctggga cttggcgtca tgctcaccga
     1921 attccacggt gaacgacatg gccgccgcca gcaccttgag ttcgtactga cgccctgcca
     1981 gccgggccag gcccgccgga ttgaaatgga tggaatcgat acccggagga tcggcggtaa
     2041 cggcgttgcc cagggccagg gccttggcat tgcccatggt gaggttttcc accagcgcgg
     2101 cggttgcggg cggcgccagc aggccggcaa ccaccgccat gaccaccgcg ccgctggcac
     2161 ggcgcccctt tgcgcactgc tgggcgtact gctggcaatc ctgatgacga acgtcagcca
     2221 tcacatcccc cctgtaccgt tgcttcaatg caactttatt tattgttttt gcttgttttt
     2281 agttgttgga ccgggaacac cggccaggca gaacaattga gcacctgctc agtctaaacc
     2341 aggcgaatca tccggggaaa gcaaaatgtg taaagcctgt ttactaaaat gatctgcagc
     2401 aatgacggtt gccgtcaggg acggcggacg cgaggggcgg gacaacaaaa ggggtgatgc
     2461 gatcacccct tggcgggtcg ggaagcgatc tcaggtcgca aaatactgcg cctggttctg
     2521 caggatgtga tcggcaatgt agctggccag ggtgtacacg gtgacctgcg gattgacgat
     2581 gatcgacgtg gggaacaggc tggcgtcggt gatgaacagg ttcttcacat cgtgggtttc
     2641 gccgtggggc gtgcacaccg aggtggcggc gtcgctgccc atgcggcagg tgccct
//
LOCUS       DASUEG010000156         8919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1732897, whole genome shotgun sequence.
ACCESSION   DASUEG010000156 DASUEG010000000
VERSION     DASUEG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732897"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFX11_02950"
     CDS             complement(<1..611)
                     /locus_tag="VFX11_02950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4937603.1"
                     /translation="MDQRKFPRQGLGVEAIFVIADDGGNNGIIQDFNQEGLFITYGDD
                     DQFIELKRRRIDPGTALDVLLEQDGRQISIAGRIVHINDSGIGVTLTGDKRNSYQMLD
                     SARRRHDAAALDTANQFVSGHRISDEARKIKILNACNDHLKAFLNEMLQEYFTVLDEA
                     LIKEAGKQKNDAAQHPYFDAIAYFKRSRSKLPELVIGKLVSDA"
     gene            complement(700..1740)
                     /locus_tag="VFX11_02955"
     CDS             complement(700..1740)
                     /locus_tag="VFX11_02955"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="HEX4937604.1"
                     /translation="MTNPALRIQLLRKLWVHLTPREMFGLTSPDRVQRAGQNLGEVRR
                     KELANLLGIERQLHELRRGHASLDAGGKIIKSAEIRDIREVKFSSIIEAAEQQEVQSE
                     VLPDLPKMLENALEIARYDQLERLLRLQKLYLLTERIILSLDVDDVDTGALDRRWFNR
                     WKLNACEISNGALQQLWARILVRELRAPGSTSLKALEYLSFFTLQDAENLNRIASWAL
                     DDFIYRSALQALPNHFDADLFDRLEEQGILRGVFGKVLSKALLSTRVDSFECQLALAD
                     CRLIVSSPQPRTELHVPAYIITRVGRELLSLVAPPADTDYLDLVRQDLQARGMTVRVA
                     ELPRDTSSLEVG"
     gene            complement(1828..2628)
                     /locus_tag="VFX11_02960"
     CDS             complement(1828..2628)
                     /locus_tag="VFX11_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4937605.1"
                     /translation="MNPSLKFQLAADDIALITLNRPDLRNAIDEDMIDGIERAVQKVN
                     SEDNIKVVVLTGAGPAFCAGGNVKDMQAKTNIFGGTPAQIRTNYQRFIQRIPLALADL
                     RVPAIAAVNGAAYGAGCDMSLMCDMRIASEQATFAESFIKLGLIPGDGGAWFLPRAVG
                     RARAAEMSFTGDAIDAKTALEWGLVNRVVSAEQLLPEALELARRIARNPATAIRMTKQ
                     LLKESERQSLASLLELSAGLQSIAHFTKDHAEAVNAFLEKRAPVFTGD"
     gene            complement(2687..2995)
                     /locus_tag="VFX11_02965"
     CDS             complement(2687..2995)
                     /locus_tag="VFX11_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937606.1"
                     /translation="MKTAERTHADLAGEELDYQMYCHACSVTGQAASRQAFEQGYGEG
                     RFHFHQDKALLADLLETYRINVQYLAESWLASNLVASAWGSTPSEAVCRLVLILRSKS
                     "
     gene            complement(3093..4730)
                     /locus_tag="VFX11_02970"
     CDS             complement(3093..4730)
                     /locus_tag="VFX11_02970"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HEX4937607.1"
                     /translation="MDTIRTVSTQPFKDQKPGTSGLRKKVRVFQQPHYLENFVQSIIN
                     ALPESQRQRLVLGGDGRYYNREAIQIILEILVANGVGEVLVGEEGILSTPAVSNLIRQ
                     YQADGGIVLSASHNPGGPDEDFGIKFNNASGSPAPESLTERMYQETLSISQYRRLPLP
                     PLELKAGREYRHGNTLIRVLDSVDDYAALMTRLFDFDRLQRAIRQGKVSLTFDAMHAV
                     TGPYARRIFHDMLELPAHLLRNVQPLPDFGAGHPDPNRVHAHELWDAMMADGAPDLGA
                     ASDGDGDRNMIMGRNIFVSPSDSLALIAEHHRCIPQFRSGLAGVARSMPTSTAIDRVA
                     KHLKVPCYETPTGWKFFGNLLDAGKVRLCGEESFGTSGDHVREKDGVWAVLCWLSILV
                     ETGLPVEELLLRHWHAFGRSHYCRHDYEGLDAAAADAVLQGLLAQASSLRGTSCDSAS
                     IATVDSFTYEDPVDGSVSRNQGVRLLFDNGSRIIYRLSGTGTGSATLRVYLEQLEQRR
                     DKLALDSIEHNRRLGQFAHQVSRIQALTGAIEPSVIT"
     gene            4898..6268
                     /gene="ppnN"
                     /locus_tag="VFX11_02975"
     CDS             4898..6268
                     /gene="ppnN"
                     /locus_tag="VFX11_02975"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEX4937608.1"
                     /translation="MTPKIDCVTVSPEASLELLSPSEVDQLVRQTDPTLFRTFRRCAL
                     AVLNTGNVNDNTAEILEEFKDFDIRFIRQARGIKLQLRHAPASAFVDGRMIRGIREQL
                     FSVLRDVIYVTNEIRNSHRFNLNTRNGITDAVFHVLRNANVLHKGETNPLIVCWGGHS
                     ISRDEYDFTKKVGYELGLRDMNIITGCGPGAMKGPMKGATIGHSKQHIVNRRYIGITE
                     PGIIAAESPNPIVNELVIMPDIEKRLEAFVRTAHGVIVFPGGVGTAEEILYILGLLLH
                     PSNTRMPFPLIFTAAESSASYFDTINEFIQQTLGEQARARYEIIIGDAAQVARSIKSG
                     SDEVFQFRRKHKDAFYFNWKLNVPKELQSPFEPNHDTMRKLNLHKDQPVHQLAANLRR
                     AFSGIVAGNVKEHGIKLIREKGPFELEGDPEIMEPLDKLLRTFVEQNRMKIGEGTYKP
                     CYRVVR"
     gene            6269..7030
                     /gene="ubiG"
                     /locus_tag="VFX11_02980"
     CDS             6269..7030
                     /gene="ubiG"
                     /locus_tag="VFX11_02980"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX4937609.1"
                     /translation="MKSVSPATLGNTGDAKEIARFNALAATWWNPSGPMWPLHKMNEF
                     RVQVILDVLHVQGLIDRHQPQPLQGLQVLDIGCGGGILSEALARLGARVTGIDLAERN
                     IRIAQQHAASSGLNIEYRVQDVTQLRESFDLIFNMEVVEHVGDLPTFMQACQAQLKPG
                     GITIASTINRTLLSYLIAILGAEYVLKLLPKGTHRWRKFVTPQELETLLNAGGVDCFW
                     RAGIRVNPWRRSFALHPSMAVNYMLAGKKQAWLAV"
     gene            complement(7044..7808)
                     /locus_tag="VFX11_02985"
     CDS             complement(7044..7808)
                     /locus_tag="VFX11_02985"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX4937610.1"
                     /translation="MTDTDTGARPGKTLVVPSRKLEWSAPLRWLQLGWQDYRSAPRLS
                     IEYGLFFAVGGLVLTVLIMLFGDKILLFSLGGLFVLLGPLFAFGLYDVSRQLQKGETP
                     TLRHSIGQIRSSAPNQWVFAVVVIVIALVWMRAATIITIFYPDDPDPALEELLTFFAI
                     GTGAGAFFAGLVFGISAFSLPMMVDRNVDAITAALTSLRAVMNNSGVAIFWGFLIVLL
                     VTLGFATFYLGLILVLPLIGYATFHGYRDTIPRPPN"
     gene            complement(7828..8358)
                     /locus_tag="VFX11_02990"
     CDS             complement(7828..8358)
                     /locus_tag="VFX11_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4937611.1"
                     /translation="MKAAGLERVHMLQGVPTFGGINDSVLSFLLRESRTINLSAGTLI
                     FAEGEYTASMFIVEDGEVAIYKHKDGRDCIMAILGEGECLGEMALFDYMPRSASAVAK
                     TDCRLIEITSQNLYGVYQMDMEQFALIQMNLGREIARRLRKADDLCMKCPLRADTEVK
                     TFRPVSAGKQEKSGTH"
     gene            8504..>8919
                     /locus_tag="VFX11_02995"
     CDS             8504..>8919
                     /locus_tag="VFX11_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937612.1"
                     /translation="MLRLQSRLRQLACCLLLTASPWSLAEDVNTVNSEMQKAGSALSA
                     LIPFIYNDTRFRNPGNREMIELQLDQLIRSMQASPNLMNDHALLRRLSQTSVQNQLVQ
                     ARDLFKSGNYATAQYLLSGVPVLCSNCHIQDGITAS"
BASE COUNT         1865 a         2748 c         2535 g         1771 t
ORIGIN      
        1 tgggcgtcgg aaaccagctt gccgatcacc agctccggca atttgctgcg gctgcgcttg
       61 aagtaggcga tggcatcaaa atacggatgt tgcgcagcat cgttcttctg ctttcccgct
      121 tccttgatca gcgcttcatc cagcacggta aaatattcct gcagcatttc gtttaaaaat
      181 gctttcaggt gatcgttgca ggcattcaga atcttgatct tgcgcgcttc gtcggaaatg
      241 cggtggccac tgacaaactg attcgccgta tccagtgcgg cagcatcgtg gcggcggcgt
      301 gccgaatcca gcatctggta gctgttgcgt ttgtcgccgg taagggtaac gccgatgccg
      361 ctgtcgttaa tgtgcacgat cctgccggcg atgctgatct ggcgtccgtc ctgctccagc
      421 aacacatcca gtgcggtacc gggatcaatg cggcgacgct tgagttcgat gaactgatcg
      481 tcgtcgccgt aggtgatgaa cagtccttcc tgattgaagt cctggatgat cccgttgttg
      541 ccaccatcgt ctgcgatgac aaaaattgcc tcaaccccca acccctgcct gggaaatttg
      601 cgctgatcca cgacaccacc catcgttaaa cgtcaaagtt cgacttgtcc aaaacaagtt
      661 tagacaaaaa cgggaggatt agaggttatt cgaggaaaat cagccaactt ccaggctgga
      721 ggtgtcccgc ggcagctcgg caacgcgcac ggtcatgcct cgggcctgca gatcctgcct
      781 gaccagatca aggtagtcag tatccgccgg tggcgcgacc agactcagca gctcgcgccc
      841 aacccgtgtg atgatgtagg ccggcacatg gagctccgta cggggctgcg gactgctgac
      901 gatcaggcgg cagtcggcca gcgccagctg gcattcgaag ctgtccacac gggtgctgag
      961 cagggctttc gacagcacct tgccaaacac cccacgcagg atgccctgct cttccagccg
     1021 gtcgaacaga tctgcgtcga agtgattggg cagggcctgc aatgcactgc gatagatgaa
     1081 atcatccagc gcccacgagg caatgcggtt gagattttcc gcatcctgca aagtaaaaaa
     1141 agacaggtat tccagcgcct tcagactggt gctgccgggg gctcgcagct cacgcaccag
     1201 aatgcgcgcc cacagctgct gtaacgcgcc attggaaatc tcgcaggcgt tgagtttcca
     1261 gcgattgaac cagcgccggt ccagcgcacc ggtgtcgacg tcatcgacgt ccaggctgag
     1321 aatgatgcgc tcggtaagca aatagagttt ctgcagccgc agcaggcgtt ccagctggtc
     1381 gtatcgcgca atttccagcg cgttttccag cattttgggc agatccggca gcacctcgct
     1441 ctgaacttcc tgctgctcgg ccgcctcgat gatggaggag aacttcactt cgcggatatc
     1501 gcggatctcc gctgacttga tgattttgcc accggcatcc aggctggcgt gaccccgtct
     1561 cagttcatgc agctgccgct cgatgcccag cagattggcc agttccttgc gtcgcacttc
     1621 acccaggttc tgacccgccc gctgtacccg gtcaggggac gtcaggccaa acatctcccg
     1681 tggcgtcaga tggacccaca gctttctcaa caactggatt ctgagtgccg gattagtcac
     1741 tccgccccct cggcaggttg ttttgctaaa aaccacccaa atggccaacg caatgaagag
     1801 tatagcgttt gccggcaatt gtgcaggtca gtcgccggtg aacaccgggg cacgcttctc
     1861 cagaaaggca tttacggctt ccgcatgatc cttggtgaaa tgggcaatgg actgcaggcc
     1921 ggcagacaat tccagcaggc tggccaggga ctggcgttcg ctctctttca gcaattgttt
     1981 ggtcatgcgg atggcggtgg ccggattgcg ggcaatgcga cgggccagct ccagcgcttc
     2041 cggcagcagt tgctccgcgc tgaccacacg attcaccagc ccccattcca gggcggtttt
     2101 ggcgtcgatg gcgtcgccgg tgaagctcat ctccgccgcc cgcgcccgac ccacagcccg
     2161 gggcaggaac caggcgccgc cgtcaccggg aatcaggccc agcttgatga agctctccgc
     2221 aaaggtggcc tgttccgagg caatgcgcat gtcgcacatc agactcatgt cgcagccggc
     2281 accgtaggcc gctccgttga ctgccgcgat ggcgggcacc cgcagatccg ccagcgccag
     2341 gggaatgcgc tggataaagc gctggtagtt ggtgcggatc tgggctggcg tgccgccgaa
     2401 aatattggtt ttcgcctgca tgtccttgac gttgccgccg gcgcaaaaag ccgggccggc
     2461 gccggtgaga accaccacct tgatgttgtc ctcgctattg accttctgca ccgcacgttc
     2521 gatgccgtcg atcatgtcct catcgatggc gttgcgcagg tccggacggt tcagggtgat
     2581 cagcgcgatg tcgtcagcgg caagctggaa tttcagggac ggattcatgg cgtctccatg
     2641 gttacgcgta ggattggcag ccggtcggca agcgaccgct gccacatcag gattttgatc
     2701 gcaggatcag caccaggcgg caaacggcct cgctgggcgt gctgccccag gcgctggcca
     2761 ccagattcga cgccagccag ctctccgcca ggtattgcac attgatgcgg taggtttcca
     2821 gcaggtctgc cagcagtgcc ttgtcctgat ggaagtgaaa cctgccttcc ccataaccct
     2881 gctcgaatgc ctgacgggac gcggcctgac cggtgacgga gcaggcatgg caatacattt
     2941 ggtagtccag ttcctcgccg gccagatcgg catgcgtgcg ttcggctgtc ttcatcgttg
     3001 attccagggc ttgtcgttgg aaagtcaggg attctgtgga ttgccaccgg ccccgtcaag
     3061 agcaggtggc gctcggcagc ggtcaaaccg gctcaggtga taaccgaggg ttcgatcgca
     3121 cccgtcaggg cctgaatgcg gctgacctga tgggcgaact gccccaggcg ccggttgtgt
     3181 tcaatggaat ccagcgccag cttgtcgcgc cgctgttcca gttgctccag atacacccgc
     3241 aaggtggcgc tgccggtacc cgtgcccgag agccggtaaa tgatgcggga accattatcg
     3301 aacagcagac gcacgccctg attgcgggaa acactgccat cgaccggatc ttcgtaagtg
     3361 aaggaatcca ccgtggcaat gctggcagaa tcgcaggagg tgccgcgcag ggaactggct
     3421 tgcgccaaca atccctgcag caccgcgtcg gccgcggcgg catccagccc ttcatagtca
     3481 tgacggcaat aatggctgcg gccaaacgca tgccagtgcc gcagcagcag ctcttccacc
     3541 ggcaaaccgg tttccaccag aatgctcagc cagcacagca ccgcccagac accatctttt
     3601 tcccgcacat ggtcgccact ggtaccaaag ctttcctcac cgcacaggcg cactttgccg
     3661 gcgtccagca ggttgccaaa gaacttccag ccagtaggcg tttcgtaaca gggcactttc
     3721 aggtgcttcg ccacccggtc gatggcggtg ctggtgggca tggaacgggc cacacccgcc
     3781 agaccggagc ggaactgggg aatgcagcga tggtgctcag cgatcagggc caggctgtcg
     3841 ctggggctca cgaaaatgtt gcggcccatg atcatgttgc ggtcgccatc accgtccgag
     3901 gcggcgccca ggtccggcgc accgtcggcc atcatggcat cccacagctc gtgggcatgg
     3961 acgcggttgg gatcggggtg gccggcaccg aaatccggca gcggctgcac gttgcgcagc
     4021 aggtgggccg gcaattccag catgtcgtgg aaaatacggc gggcgtaggg cccggtcacg
     4081 gcgtgcatgg cgtcgaaggt caggctcacc ttgccctggc ggatggcccg ctgcagccgg
     4141 tcaaaatcaa acagccgggt catcagcgca gcgtaatcgt ccacgctgtc cagcacgcgg
     4201 atcagggtgt tgccatggcg gtattcgcga ccggccttca attccagtgg cggcagtggc
     4261 aggcgcctgt actggctgat ggacagggtt tcctggtaca tgcgctcggt cagggattcc
     4321 ggcgccgggc tgccgctggc attgttgaac ttgatgccga aatcctcgtc cggcccgccg
     4381 ggattgtggc tggccgacag cacgatgcca ccatccgcct ggtactgccg gatcaggttc
     4441 gacactgccg gcgtcgacag gatgccctcc tcgcctacca gcacctcgcc caccccattg
     4501 gccaccagga tttccagaat gatctggatc gcctcccggt tgtagtagcg gccgtccccg
     4561 cccagcacca ggcgctggcg ctgtgactcg ggcagggcat tgatgatgga ctggacgaaa
     4621 ttctccagat aatgcggctg ctggaacaca cgcaccttct tgcgcaggcc ggaggtgccg
     4681 ggcttctgat ccttgaaggg ctgggtactg acagtgcgaa tggtgtccaa gggtgactcc
     4741 ctgtatgctg gtgggctttg ccaaaaaagc ccgtctcacg gagatgccgg tttgctatag
     4801 ttaccggtac tgaatccgat aatcgtaaaa taattaagcg tttactgcat tagtgttttg
     4861 cttgtatcca atttgctctc aaggaatccc gctcttcatg acacccaaga tcgattgcgt
     4921 tacggtttcc ccggaagcct cgctggagct gctgtccccc tctgaagtcg accagctcgt
     4981 ccggcagacc gaccccacgc tgttccgcac cttccgccgc tgtgcactgg cggtgctgaa
     5041 taccggcaac gtgaatgaca acaccgccga gatcctggaa gagttcaagg atttcgacat
     5101 ccgcttcatc cgccaggccc ggggcatcaa actgcagctg cgccacgccc ccgccagtgc
     5161 cttcgtcgat ggcagaatga tccgcggtat tcgcgaacag cttttttcag tgctgcggga
     5221 cgtcatttat gtcaccaacg aaatccgcaa ctcgcaccgt ttcaacctga atacccgcaa
     5281 tggcatcacc gatgcagtat tccacgtgct gcgcaatgcc aacgtcctgc acaaaggtga
     5341 aaccaatcct ctcatcgtct gctggggtgg ccactccatc agccgcgacg aatacgactt
     5401 caccaaaaaa gtgggctatg aactgggcct gcgcgacatg aacatcatca ccggctgcgg
     5461 ccccggcgcc atgaaaggcc ctatgaaagg tgccaccatc ggccattcca agcagcacat
     5521 cgtcaaccgg cgctatatcg gcatcaccga gcccggcatc atcgcggcag agtcgcccaa
     5581 ccccatcgtc aacgaactgg tgatcatgcc ggacatcgaa aaacgcctgg aagccttcgt
     5641 gcgcacggct catggcgtga tcgtgttccc tggcggcgtc ggcacggcgg aggaaatact
     5701 ctacatcctg ggattgttgc tgcacccttc caacacccgc atgcccttcc cgctgatttt
     5761 caccgccgcc gaatccagcg ccagctattt cgacacgatc aatgaattca tccagcagac
     5821 cctgggcgag caggcccgcg cccgttacga gattatcatc ggcgacgcag cgcaggtggc
     5881 acgctccatc aaatccggct ccgacgaggt tttccagttc cgccgcaagc acaaggatgc
     5941 gttctacttc aactggaaac tgaacgtgcc gaaggagctg caatcaccct tcgagcccaa
     6001 ccacgacacc atgcgcaaac tgaacctgca caaggaccag ccggtgcacc agctggcagc
     6061 gaatctgcgc cgcgcgttct ccggcatcgt agccggcaac gtgaaggagc acggcatcaa
     6121 actgattcgc gagaaaggcc cgttcgaact ggaaggcgat ccggaaatca tggagccgct
     6181 ggacaaactg ctgcgtacct tcgtcgagca gaaccggatg aaaatcggcg aaggaaccta
     6241 caagccctgc tatcgggtgg tgcgctgagt gaaatccgtt tcacctgcta ccctgggtaa
     6301 caccggcgac gcgaaagaga tcgcgcgttt caatgcgctg gccgcgacgt ggtggaaccc
     6361 gtccggcccg atgtggccgt tgcacaagat gaacgaattc cgggtgcagg tcattctgga
     6421 cgtgctgcat gtacaaggat tgattgaccg gcaccagccg caacccttgc aaggcctgca
     6481 ggtgctggat atcggttgcg ggggcggcat tctgtcggaa gcgctggcgc ggctgggagc
     6541 gcgggtcacc ggcatcgatc tggcggaacg caatatccgc atcgcgcagc aacacgcagc
     6601 cagttccggg ctgaatattg aataccgcgt ccaggatgtc acccagctgc gggaaagttt
     6661 cgacctgatt ttcaacatgg aagtggtgga gcatgtgggc gacctgccca ccttcatgca
     6721 ggcctgtcag gcccaactaa aacccggcgg catcaccatt gcatccacga tcaatcgcac
     6781 gttactgagt tatctgattg ctatcctcgg cgcggaatat gtgctgaaac tgctgccgaa
     6841 aggcactcac cggtggcgca agttcgtgac accgcaggaa ctggaaacat tgctgaatgc
     6901 cggcggcgtc gactgtttct ggcgcgccgg aatccgcgtg aacccctggc gacgcagctt
     6961 cgcgctgcat ccgtcgatgg cggtgaatta tatgctggca ggaaagaagc aggcctggct
     7021 ggcagtttga tccggctggc cactcagttc ggcgggcgtg gaatcgtatc ccggtagccg
     7081 tgaaatgtcg cgtaaccaat cagcggcagc accagaatca aacccagata gaaagtcgca
     7141 aatcccagcg tcaccagcag cacgatcaga aaaccccaga aaatcgccac gccactattg
     7201 ttcatcaccg cgcgcagact ggtgagcgcc gccgtgatcg catccacatt gcgatccacc
     7261 atcatcggca gtgaaaacgc gctgatcccg aacacgaggc ccgcgaaaaa cgcgcccgct
     7321 ccagtgccaa tggcaaagaa cgtcagcagc tcctccagcg cagggtcagg atcgtccgga
     7381 taaaaaatcg taatgatggt ggctgcccgc atccagacca gcgcgatcac gatcaccact
     7441 acggcaaata cccattggtt gggcgcactg ctgcggatct ggccgatgga atgacgcaag
     7501 gtgggggttt cgcctttctg caattgccgg ctcacgtcat acaggccaaa tgcgaacaga
     7561 ggtcccagca acacaaacag tccacccagg ctgaacagca ggatcttgtc gccaaacagc
     7621 atgatcagta ccgtgagcac cagaccgccc accgcaaaaa acaaaccgta ctcgatgctg
     7681 agccggggtg cacttctgta atcctgccaa cccagctgca gccagcgcag cggggctgac
     7741 cactccagtt tgcgggatgg caccaccagc gttttgcccg gtctggcgcc ggtatccgta
     7801 tcggtcattg ttccccctgc ctgctggtca gtgtgttcct gacttttctt gttttcctgc
     7861 tgatacgggc cggaacgtct tcacttcggt atcggcgcgc agcggacact tcatgcacaa
     7921 atcatccgcc ttgcgcaggc ggcgggcgat ctcgcggccc agattcatct ggatcagcgc
     7981 gaactgctcc atgtccatct ggtaaacgcc atacaggttc tgtgaggtta tttcgatcag
     8041 gcggcaatcc gttttggcca cggcggaagc actgcgaggc atgtaatcga acagggccat
     8101 ctctcccaga cactcgccct cccccagaat ggccatgatg cagtcgcgtc catccttatg
     8161 tttgtagatc gcgacttcgc cgtcttcaac gatgaacatc gacgcggtat attccccttc
     8221 agcaaagatc agagtccctg ccgacaggtt gatcgttctg gactcccgca acagaaacga
     8281 caataccgaa tcgttgatgc ctccaaaggt gggaacaccc tgtaacatat gaacccgttc
     8341 cagaccggct gctttcatgg ttcttgtccc tgtgaacagt tagttcaact ataggccaag
     8401 caacaggttt tctcagcaac aattgatttg cgccgctggc cagagcgata aatggctgat
     8461 aattattcga ccagtgtcga ccacatccaa ccaaggaatc atcatgctcc gcctccaatc
     8521 ccgtctgcgg caattggcct gctgcttact cctgacggcc agtccctggt cgctggccga
     8581 agatgtcaat accgtcaatt ccgaaatgca aaaagccggc agcgcgctgt ctgcgctgat
     8641 tccattcatt tataacgaca cccgcttccg gaatccgggc aaccgggaaa tgatcgagct
     8701 gcagctggac cagctgatcc gctcgatgca ggcttcaccc aacctgatga acgatcacgc
     8761 gctgttgcgc cgcctgtccc agaccagtgt gcagaatcag ctggtacagg cccgcgacct
     8821 gttcaagtcc ggcaattacg ctactgccca atacctgctg agcggcgtgc cggtgctgtg
     8881 cagcaactgc cacatccagg acggcatcac cgccagcag
//
LOCUS       DASUEG010000157         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_448901, whole genome shotgun sequence.
ACCESSION   DASUEG010000157 DASUEG010000000
VERSION     DASUEG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448901"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFX11_03000"
     CDS             complement(<1..341)
                     /locus_tag="VFX11_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937613.1"
                     /translation="MNKPAEATADKSALKPKTPFSFRIDLRWCLDELLKDGRISQRDA
                     NLVSTTPRGREQLNWHPLQVIAHFDFEDARGGGKLTLDALTVWLAEKAGQPVFHIDPL
                     KIRAGDVTSIMS"
     gene            454..1101
                     /locus_tag="VFX11_03005"
     CDS             454..1101
                     /locus_tag="VFX11_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4937614.1"
                     /translation="MRHFLATLLALATLALGGCGYNTMQAQDEAINAAWAEVLNQYQR
                     RADLVPNLVNVVKGYAAHEAGVLIAVTNARANVGSIKADASLLNDEAAFAKFQAAQGE
                     MTSALSRLLVVAENYPNLKADANFRDLQAQLEGTENRITVARNRYIKEVQAYNTTVRS
                     FPSNFTAMAFGLKTRPTFSVQNEAAISAPPVVDFSAPAAPAPAPAPAAPAPAPAP"
     gene            1150..2010
                     /locus_tag="VFX11_03010"
     CDS             1150..2010
                     /locus_tag="VFX11_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4937615.1"
                     /translation="MRLLPLLFAWLIWLPSLAVAVEGEPQPVPALKAPVTDLAELLPP
                     AEEEQLNARLLAFSQEKGSQIAVLIVPTTQPEDIFSYSFRVADTWKLGRKGVDDGVLL
                     VVAVQDRKTHLQVGYGLEGAIPDARAKQILQDIIRPHFKAGNFPAGINAGVDAVITLI
                     NGEQQPAPAAQGGRSDPQQNPIVLALVIGVIAGLFLRAALGRLIGGLSGGGIALTIAL
                     FLGAALGTAVIVAVIVLLFAALAGDGRRLGGYHGGFGGGGFGGGSFGGGGGFSGGGGG
                     FGGGGASGDW"
     gene            2053..2679
                     /locus_tag="VFX11_03015"
     CDS             2053..2679
                     /locus_tag="VFX11_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4937616.1"
                     /translation="MPSSRIFTLGPDVPRRGNTFSHLCGLFLLWLIGWRMRGDFPNVR
                     KGMVIVAPHTSNYDGVVAVAAILALRLQLLFFVKDSAFVWPIGGLMKWFGARPVDREN
                     SKDLVGYTVRQYAANDSLMLAIAPEGTRKASPAWKTGFYWMAYQAGVPIIMIAFDYSK
                     KEIVVLGCFTPTGNVEQDLPLIIEHFRGMEPCHEERLSAPLRALREKR"
     gene            complement(2753..2938)
                     /locus_tag="VFX11_03020"
                     /pseudo
     CDS             complement(2753..2938)
                     /locus_tag="VFX11_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
     gene            3186..>3752
                     /locus_tag="VFX11_03025"
     CDS             3186..>3752
                     /locus_tag="VFX11_03025"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX4937617.1"
                     /translation="MKFLRTPDERFAQLPGYPFAPHYVMVDDTEGGELRMHYLDEGPA
                     GAPVVLMLHGEPSWSYLYRKMIPVVAAAGYRVIAPDLIGFGRSDKPEQRTDYTYQRHV
                     DWVRSLLQQLQLRDITLVCQDWGGLIGLRLVGEHPDWFARVVAANTMLPTGDFHPGDA
                     FLKWQEYSQTTPVFNTGKIVAGGCATPLS"
BASE COUNT          673 a         1184 c         1129 g          766 t
ORIGIN      
        1 gacatgatgg aggtgacatc accggcccgg atcttgagcg gatcgatgtg aaacaccggc
       61 tgcccggcct tctcggccag ccataccgtc agcgcatcca gcgtcagctt gccgccccct
      121 ctggcatcct cgaaatcgaa atgcgcgatg acctgcagcg gatgccagtt gagctgttcg
      181 cgaccacgcg gggtcgtcga gaccaggttg gcatcacgct ggctgatgcg gccatccttg
      241 agcaactcgt ccaggcacca gcgcaggtcg atgcggaagg agaatggcgt tttgggcttg
      301 agcgccgact tgtcggctgt cgcttctgcg ggcttgttca tgttctgtgg ccccgttgga
      361 atgctgttga ctataacgcc tgccagcgcc cttgtaattc ctgtcgtccg ccttatcttg
      421 tgaacatcgg taaccagtga aggacatcac accatgcgcc acttccttgc caccctgctt
      481 gccctggcca ccctggccct tggcggctgc ggctacaaca ccatgcaggc tcaggacgaa
      541 gccatcaatg ctgcctgggc cgaagtgctc aaccagtacc agcgtcgtgc cgatctggtg
      601 cccaatctgg tcaacgtggt gaagggctat gccgcccacg aggccggcgt gctgattgcc
      661 gtaaccaacg cccgcgccaa tgtcggcagc atcaaggccg atgcctcgct gctgaacgac
      721 gaagctgcgt ttgccaagtt ccaggcggcg caaggcgaga tgacctcggc tctgtcgcgc
      781 ctgttggtgg tggccgagaa ctatccgaac ctcaaggcgg atgccaactt ccgcgacttg
      841 caggcccagc tggaaggcac tgagaaccgc attaccgtgg cccgtaaccg ctacatcaag
      901 gaagtgcagg cctacaacac caccgtgcgc tccttcccgt ccaacttcac ggccatggcc
      961 tttggcctga aaacgcgccc gaccttctcc gtgcagaacg aagcggcaat cagtgcacct
     1021 cctgtcgtgg acttcagcgc acccgccgct ccggcaccgg caccagcccc cgcagcccct
     1081 gctccggcac cggcgccttg atgcggcaag ggcaggcatg acgcctgccc gtttgccatt
     1141 ggacatccca tgcgcctgct cccactcctc ttcgcctggc tgatctggct gccttcgctg
     1201 gcagtggctg tcgagggcga accgcaaccc gtcccggccc tgaaggcgcc ggtcacggat
     1261 ctggccgagc tgttgccgcc tgctgaagaa gagcagctga atgcccgcct cctggcgttc
     1321 tcgcaggaaa aaggcagcca gattgccgtg ctcatcgtgc ccactaccca gcccgaggac
     1381 attttcagct acagcttccg cgtggctgac acctggaagc tcgggcgcaa gggcgtggat
     1441 gacggcgtgc tgctggtggt cgccgtacag gatcgcaaga cccacctgca ggtgggctac
     1501 gggctggaag gcgccattcc ggatgcgcgt gcgaaacaga ttctgcagga catcatccgt
     1561 ccgcacttca aggccgggaa ctttccggcc ggcatcaatg caggcgtgga cgccgtcatc
     1621 accctgatca atggcgagca acagcctgca cccgccgccc aaggcggccg ctccgacccg
     1681 cagcagaacc cgattgtgct ggccctggtc atcggcgtga ttgccggcct gttcctgcgc
     1741 gcggcgcttg gccgcctgat tggaggactg tccggcggcg gcattgccct caccattgcc
     1801 ctgtttctgg gtgctgcact gggtacggcc gtcatcgtgg cggtgattgt cttgctgttt
     1861 gcggcattgg cgggtgatgg ccgccggctg gggggctatc acggcggctt tggcggaggc
     1921 gggttcggtg gcggcagttt tgggggtggc ggcggatttt ccggcggtgg cggaggcttc
     1981 gggggcggcg gcgcctctgg agactggtaa gataactacc attacaacaa catcacttcc
     2041 ccttattcag cgatgcctag ctcccgaatt ttcaccctgg ggcccgatgt cccccgacgc
     2101 ggcaatacct tcagccatct gtgcggcctg ttcctgctct ggctgatcgg ctggcgcatg
     2161 cgtggcgatt tccccaatgt gcgcaagggt atggtgatcg ttgcgccgca tacctccaac
     2221 tacgatggcg tggtggcggt ggctgccatc ctggccctgc gcctgcaact gctgttcttc
     2281 gtgaaggata gcgcctttgt gtggccgatt ggtgggctca tgaaatggtt tggcgcccgt
     2341 cctgtggacc gtgaaaactc caaggatctg gtgggctaca cggttcgcca gtatgccgcc
     2401 aacgactcgc tgatgctggc gattgcgccc gagggtacac gcaaggcctc tcccgcctgg
     2461 aaaaccggct tttactggat ggcctaccag gccggcgttc ccatcatcat gatcgccttc
     2521 gactattcga aaaaggaaat tgtggtgctg ggctgcttta cgcccaccgg caatgtcgag
     2581 caggacctgc ccctgatcat cgaacatttt cgcggcatgg aaccctgcca cgaggagcgg
     2641 ctttccgccc cgttgcgggc actgcgcgag aaacgctgac ccacacactt ttcttcagga
     2701 cctgtataga tgacagggcg atgccgctct ccgcgacatc gccgtgcggg acttactggc
     2761 aggatgcctg cggggtattc accactaccg tgctgtagtg ataggtgtaa tcgcccgtga
     2821 tggcctgggt gccgtcaaca ccggcgtttt ctccaatgat ccaggtcccc gccttgggaa
     2881 ttccaccatc ggcatacgcc gcaatatccg tcgtcgcact gccataggca cggatgataa
     2941 cgcgcgcatg acaactccgt tttatggttg ctggtttccg ttgcgactta tccgaggaga
     3001 gattgagtcg cgaatgtttc tagcacccat gtacagaagg atgaatcacg caaaatgcag
     3061 acggaacacc tttgcggaca ccagacaagc gatggtgctt gtcccctccc gaagaagggg
     3121 gcagcgaccc tgaggcaatt cccctatgct gttgccagac aacgatgcac attcgggaga
     3181 actccatgaa gttcctgcgc acccctgacg aacgttttgc ccagctgcct ggctaccctt
     3241 ttgcaccgca ctacgtgatg gtagatgaca cggaaggcgg cgaactgcgc atgcattatc
     3301 tggatgaagg ccctgccgga gcaccggtgg tcctcatgct gcacggcgaa ccgtcgtgga
     3361 gctatctgta ccgcaagatg attcccgttg tggccgccgc cggttaccgc gtgattgcac
     3421 ccgacctgat cggctttggc cgttcagaca aaccggagca gcgcacggac tacacctacc
     3481 agcgccacgt cgactgggta cgctcactgt tgcaacaact gcagctccgc gacatcacgc
     3541 tggtgtgcca ggactggggc gggctgattg gtctgcgtct ggtgggcgaa catccggact
     3601 ggtttgcgcg cgtggtggcg gccaatacca tgctgcccac cggagacttt catcctggtg
     3661 acgccttcct gaaatggcag gaatattcac agaccacgcc ggtgttcaat accgggaaaa
     3721 ttgtggcggg tggctgcgcc acgccgctgt cc
//
LOCUS       DASUEG010000158         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_537800, whole genome shotgun sequence.
ACCESSION   DASUEG010000158 DASUEG010000000
VERSION     DASUEG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537800"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFX11_03030"
     CDS             complement(<1..516)
                     /locus_tag="VFX11_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX4937618.1"
                     /translation="MPIPLVIFHSNCLDGFGSAYAAFAHFDIKLGTSAEYLPAAHGDT
                     PPDASGRTVYLLDYVYKRAAMETLCRQAERVVVLDHHISAVEDLAGLDQAFSNLELNL
                     DMDRSGAVITWEYFHTEPAPLLLAGVQDRDLWRFEVPGSHDLNAALMSHPFSFPAWHE
                     WANDPVALQTLI"
     gene            complement(602..1828)
                     /locus_tag="VFX11_03035"
     CDS             complement(602..1828)
                     /locus_tag="VFX11_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4937619.1"
                     /translation="MPRHQLIEYPMHLRDRFADLTCQLQNHASFWRPFPFRDPRPPWT
                     QAQPDLVRQLLALPDDALHRLATIPQELTAFLAPYLPFASALAQLCELPQCSQRALPP
                     LAPRLLAGIPGRKWQQIEAFVAALPTGELPILEWCAGKSHLGFVLQHCSGLPVTALEW
                     NAALVEQANARASQWQCDLHSHTVDVLTPAADAFVLPNQQIVALHACGDLHVKLLQLA
                     ARNGVQQVQLAPCCYQKTSDEQYQPLSHAARQQPLPLSKQELHLAVTETVTAGEYQQR
                     QRLRLQAMRLGFDCLQRDVRGLDEFLPVPSLPGEWARTDFKTFCEHCAQLKSLTLPDQ
                     VNWAHYEQRGQQRLREVSALDLVRFLFRRPLEIWLALDRALFLQEQGYQVELGTFCPR
                     HATPRNILIVAARCKP"
     gene            complement(1862..2809)
                     /locus_tag="VFX11_03040"
     CDS             complement(1862..2809)
                     /locus_tag="VFX11_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937620.1"
                     /translation="MVLIALALLSGCLPKEPIVNITAAENMRRTPDGRIFISGRAALY
                     ELRQNENGQHEAVVVSRDEACDYHAGIAQLHDWIFFVCAKQAKVLKGIIFADSGKLMA
                     YSLTDGRVEPVLDLTGFQFPNGLDALVQEDAILIADEDFLGKGGVSRIRFDFSGDVPV
                     LLDYQRDWIGAAQQVYAANGVRVHDNAVYLTDIGFVKRVPLDGDGMPENAQILYRGAT
                     VLDDLDVLCEGLLVADFVLGRLIYVPFDGSRPQVTPGGLTSPSSVLAIATPQFDAGDI
                     LVTETTALSAKSGNQLVRIRRDDLGVGACRNPGESGSGS"
     gene            3019..>3243
                     /locus_tag="VFX11_03045"
     CDS             3019..>3243
                     /locus_tag="VFX11_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937621.1"
                     /translation="MPVLPIESVLAPLLQALQDHPRLVVQAPPGAGKTTRVPLALLEQ
                     PWLAGKILLIQPRRLAVYGAARRMAYTLGEK"
BASE COUNT          661 a         1012 c          974 g          596 t
ORIGIN      
        1 gatcagcgtc tgcagtgcca ccggatcatt ggcccattcg tgccaggccg gaaaggaaaa
       61 cggatgcgac atcagggccg cattcagatc gtgactgccc ggcacttcga agcgccacag
      121 gtcgcgatcc tggacaccgg ccagcagcag aggcgccggc tccgtatgga aatattccca
      181 ggtgatcacc gcgccggaac ggtccatgtc cagattcagt tccaggttgc tgaatgcctg
      241 gtccagcccg gccagatcct ccaccgcact gatgtgatga tccagcacca ccacccgctc
      301 cgcctgccgg cacagggttt ccatggccgc gcgtttgtac acgtaatcca gcaggtaaac
      361 agtgcgtccg ctggcatccg gtggcgtatc cccatgagcg gccggcaaat attccgcgct
      421 ggtgccgagc ttgatatcga aatgcgcgaa cgcggcgtaa gcactgccaa atccatcaag
      481 gcaattgctg tgaaagatga ccaacgggat gggcataaaa acgaaactcg cagactgcaa
      541 acggcaagac aatttggcgt gactatatca tgccaccgca gtcagcccgc gtttcaaaat
      601 gttatggttt gcagcgcgct gcaacaatca ggatgttgcg cggcgtggca tggcgcgggc
      661 aaaaggtgcc gagttccacc tgataaccct gttcctgcaa aaacagcgcg cggtccaggg
      721 ccagccagat ttccagtggc cgccggaaca ggaatcgcac cagatccagt gcggagactt
      781 cccgcaaccg ttgctgtccg cgctgttcgt agtgggccca attgacctga tccggcaacg
      841 tcagggattt cagttgtgcg cagtgttcgc aaaaggtttt gaaatccgtt cgcgcccact
      901 ctcccggcag cgacggtacc ggcaggaatt cgtccaggcc gcgcacgtcc cgctgcagac
      961 aatcgaatcc cagccgcatg gcctgcaggc gcaaacgctg gcgttgctgg tattcgcccg
     1021 ctgtcacggt ttccgtcacc gccagatgca gctcctgctt gctcaatggc aacggttgtt
     1081 ggcgcgccgc gtgcgacagc ggctgatact gttcgtctga agttttctgg taacagcagg
     1141 gcgccagctg cacctgctgc acgccattgc gcgcggccag ttgcagcaat ttcacatgca
     1201 ggtcgccgca ggcatgcagc gcgacaatct gttgattcgg caggacaaac gcgtctgccg
     1261 ccggcgtcag cacatccacc gtgtgggaat gcagatcgca ttgccactgg cttgcccgcg
     1321 cgttggcctg ctccaccagc gccgcattcc attccagcgc ggtcaccggc agtccgctgc
     1381 aatgctgcag cacaaagccc agatgggatt tgccggcgca ccattccagt attggcagct
     1441 cgccggtggg cagcgcagca acgaaggcct cgatctgctg ccacttgcgg ccgggaatgc
     1501 cggccagcag gcggggtgcc agcggcggca aggcccgttg cgagcattgc ggcagctcgc
     1561 acaactgcgc cagcgcggat gcaaagggca ggtacggggc gagaaaggcc gtcagttcct
     1621 gcgggatggt cgccagacgg tgcagggcat catccggcaa tgccagcagc tgccgcacca
     1681 gatccggttg ggcctgcgtc cagggtggcc gcggatcacg gaagggaaag gggcgccaga
     1741 aggatgcatg gttttgaagc tggcaggtca ggtctgcaaa acggtcgcgc aggtgcatgg
     1801 ggtattcgat cagttggtga cgcggcatta tacgccggcc atcgaatccc cgtgacagtg
     1861 ttcaggaacc gctgcccgat tcgcccggat tccggcaggc acccacaccc agatcatccc
     1921 ggcgaatccg caccagctgg ttgccggatt tcgcgctcag ggcggtggtt tccgtcacca
     1981 gaatatcgcc ggcgtcgaat tgcggcgtgg caatcgccag tacggaagag ggactggtca
     2041 ggccacccgg ggtgacttgc ggacgggaac catcgaaagg cacgtagatc agtctgccga
     2101 gcacgaagtc ggccaccagc agcccttcgc acagtacgtc cagatcatcc agcacggtag
     2161 cgccgcgata caggatctgc gcgttttccg gcataccgtc accatccagg ggcacccgtt
     2221 tgacgaagcc gatatcggtg agatacaccg cattgtcgtg cacccgcacg ccgttggcgg
     2281 cgtaaacctg ctgggccgcg ccaatccagt cgcgctggta atccagcagc accggcacgt
     2341 cgccgctgaa atcaaaccgg atcctggaca caccgccttt gccgagaaaa tcctcatctg
     2401 caatcaggat ggcatcctcc tgcaccagcg catccagccc gttgggaaac tggaaaccgg
     2461 tcaggtcaag caccggttcg acgcggccat ccgtcagcga atacgccatc aatttgccgg
     2521 aatcagcgaa tatgattcct ttgagcacct tggcctgctt cgcgcagacg aagaaaatcc
     2581 agtcatgcaa ctgcgcgatg cccgcgtggt aatcgcacgc ctcgtcacga ctgacgacga
     2641 ccgcttcatg ctggccattt tcattctgcc gcagttcata cagcgcggct cggccgctga
     2701 taaagatgcg tccgtccggc gtgcgacgca tgttctcggc ggcggtaatg ttgacgatgg
     2761 gctctttggg cagacagccc gacagcaatg ccagtgcgat taaaaccaac ggaagtccct
     2821 tgcaattcat ggcgtccacg ctttcacggt ttgttgttat tgggatggga attttttcca
     2881 gcttttgtcc aggttgcggg atactgacag cggccattgt cacacgtcaa gacggctccg
     2941 ggaccggaca tgggagcaac aggctttccc agcctgcgtc cgattgccta cactacgggc
     3001 cccgcttccg gagaaaccat acccgtgctg cccatcgaat ccgtccttgc ccctctgctg
     3061 caggccctgc aggatcaccc gcggctggtg gtgcaggcgc caccgggtgc gggcaagacg
     3121 acgcgagtac cgctggccct gctggaacag ccctggcttg cgggaaaaat cctgctgatc
     3181 cagccacgcc ggctggcggt gtacggtgcg gcacggcgca tggcgtacac actgggggaa
     3241 aaa
//
LOCUS       DASUEG010000159         7402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1966544, whole genome shotgun sequence.
ACCESSION   DASUEG010000159 DASUEG010000000
VERSION     DASUEG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966544"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFX11_03050"
     CDS             <1..511
                     /locus_tag="VFX11_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX4937622.1"
                     /translation="EFVRWQRNPELLDAWKNGTTGYPIVDAAMRQLSTLGWMHNRLRM
                     VTAMFLTKHLFIHWKEGERWFMQQLIDGDFAANNGGWQWSASTGADAAPYFRVFNPTR
                     QSERFDADGTFIRHWLPELNTLGGKQIHDPTPLERQLCSYPHPIVDHRVAVDRIKQEF
                     QSLKTVALA"
     gene            539..1468
                     /locus_tag="VFX11_03055"
     CDS             539..1468
                     /locus_tag="VFX11_03055"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX4937623.1"
                     /translation="MKSISNGIRLWFDSEATEVGNSEQVNWLRCIPFVLLHLACLAAF
                     WTGVSTFAILFCVAFFWLRMFAITAFYHRYFSHRSFKTSRTAQFLFAVLGNMSAQRGA
                     LWWAGHHRHHHQHSDQALDLHSPVQRSFWWSHMGWFTCDASFKTPYDRIRDFARFPEL
                     VFLNRYDALVPALTMLAIFSLGELLAAFAPGLETSGLQLLVWGFFISTTLLFHSTVTI
                     NSLAHVWGKRRFATGDNSRNNLALALITLGEGWHNNHHRYPASARHGFARWEIDITYL
                     LLLLLEKVGLIWELKPIPARIRQEMQSGAASVR"
     gene            1509..2813
                     /locus_tag="VFX11_03060"
     CDS             1509..2813
                     /locus_tag="VFX11_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4937624.1"
                     /translation="MEYKKIAVVGSGISGLLSALLLQKKYSVSLFEQNAYLGGHTHTL
                     DVDLDDQRFPVNTGFIVYNDWTYPNFIRLMDHLGVASEASSMSFSVRCDRSDLEYNGT
                     SFNSLFCQRKNLVSRRFWRMVSDIVRFNRQATRAHQQGDIDPGMTLQRFLDENRYSEE
                     FIRYYIIPMGAAIWSAPESDMLGFPLAFFVRFFHNHGMLSVDQRPQWRVISGGSRSYV
                     NALKQQLRGPTHIGRRVEQIERFSHGVLLRDDQGQLEQFDAVVLACHSDQALAMLTQP
                     SPEERQFLGAIPYQDNDVVLHTDIRLLPQRKLAWAAWNYRIPERADRPVHVTYNMNIL
                     QNFVSDTTFCVSLNQNDRIDPARIIARYRYSHPAYTLAGMHAQNSFDRINGKHHTYYC
                     GAYWFNGFHEDGVNSAIRAARSLGVDFDEVIAPCKAPSTKAS"
     gene            2783..3577
                     /locus_tag="VFX11_03065"
     CDS             2783..3577
                     /locus_tag="VFX11_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX4937625.1"
                     /translation="MQSALYEGVVSHRRQTPTAHEFRYPLFMLYLNLDELEDFFALSP
                     LWSLERFNWASFYRRDFLNPSVASLRDAVRLEIRQQTGHTFDGDIFLLTHIRYLGYCF
                     NPVSFYYCFEQGKLTWIVAEINNTPWNERFRYVLQCSAEENRQSFAFDKAFHVSPFLP
                     MGMQYEWQFGLPGEKLSVFMRNRQDGNEVFNATLLLQRQDATASALNRILLKFPAVTI
                     KTVAGIYWQALKLWLKRTPIYDHPLSDEATPHAILNRQQSNTTDQH"
     gene            3531..4790
                     /locus_tag="VFX11_03070"
     CDS             3531..4790
                     /locus_tag="VFX11_03070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019095041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX4937626.1"
                     /translation="MQSSTVSKATPLTSTERFCRQLLLKAFAQAEFGTLVICDPDGEY
                     ECRGSKDASAIQARIDVQDSACYRDMVLGGSIGAAEAYMTGDWTSPDLTAVIRFMSRN
                     LNALNRMESGLARLAQPFQRLYHWNNRNTEKGSRRNIAAHYDLGNDLFQLFLDPTMMY
                     SAGIFPSPYATMEQASVTKLDRICQKLDLQPHDHVIEIGTGWGGFAIHAATHYGCKVT
                     TTTLSQQQHDYARNEIRARGLEDNITLLLQDYRDLKGEYDKLVSIEMVEAVGHQYYGA
                     YFETLGRLLKPNGIGLIQAIIIDHKRYQTAIKNVDFIQRYIFPGSCIPSVNALLQNCS
                     DHSDLQLTHFEDITPHYARTLREWRHAFFRNLDKIKALGYSDEFVRMWDFYFCYCEGG
                     FEERVIGDVQLLFAKPGYRGQPVLGRL"
     gene            4792..5301
                     /locus_tag="VFX11_03075"
     CDS             4792..5301
                     /locus_tag="VFX11_03075"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HEX4937627.1"
                     /translation="MLLNAVLFQAGWLLCVMERGPLALLASALILLVHGRYVTRNRRE
                     WLFILALGSVGIALDFLFMKSGVFHIDGHDFPPPWLMLVWLLFASTLNHSLRWLDQRL
                     WLAAVLGAIAAPLSYLAGARLGALQIELHMLPVLGAAWAALLPLAFLCNRRLLLTQPP
                     TQTTGSTTP"
     gene            5298..6080
                     /locus_tag="VFX11_03080"
     CDS             5298..6080
                     /locus_tag="VFX11_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937628.1"
                     /translation="MNRSHATLLTLVVIITFPLAHARQDSPSPQTVSAKQLHYVGTAT
                     RLDTVIPAYTESYTEHYDAQGNLLSGQVRYQDAQGEPLADKQLDYRPHPHAPAFSFHN
                     RKTGYREAVIWRDDGRILLMNADAAHTLPREKILRVPEPVVADAGFNRFVQDNLARLE
                     QGERIEFHFLNPARLDWFRFSASGNVLDQQNMQVVIAPANPVLQWLVEPIRLVYARDS
                     GRLLQYEGITNISLDGGDTLVARIQYRYSMKPVEYPDTLPEG"
     gene            6084..6986
                     /locus_tag="VFX11_03085"
     CDS             6084..6986
                     /locus_tag="VFX11_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4937629.1"
                     /translation="MHSIQSVAERTGLTPDVIRIWERRYQAVAPDRTASNQRLYSDDD
                     VERLALLKRVTDNGRRISIVANLSNDELRQMAAQVQVAPRTALPAVQVDPLALQEQAL
                     DAIQQLDPALFDQLLERAMVGMGSVGFMMRFAAPLMVTVGNLWRSGAVRTCQEHFASA
                     HLRSFLGRYMLDANTDPLGPRIVIATLPGQAHELGATMAAVVAAQSGWNVLYLGANVP
                     IEEIAFAADCKDARAVAVSLSYPLDDPRVPVLLKELRQKLPSSSTLIVGGIGSTRQQP
                     LLDELKALQADDLVRLAEHLDRMR"
     gene            complement(7030..>7402)
                     /locus_tag="VFX11_03090"
     CDS             complement(7030..>7402)
                     /locus_tag="VFX11_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937630.1"
                     /translation="EIQDIKPSETMQQAMEEQAAAERQRRAAVTKAEGEKQAAILQAE
                     GRLEASRKDAESQVVLANASRDAITLVTESIKGDSLPVTYLLGEKYVNALTDLAASPN
                     TKTLVLPADMLNTVRGLMGTK"
BASE COUNT         1467 a         2293 c         2092 g         1550 t
ORIGIN      
        1 cgaattcgtg cgctggcagc gcaatcctga gttgctggac gcctggaaaa atggcaccac
       61 cggctacccc attgtggatg ccgccatgcg ccagttatca acgctgggct ggatgcacaa
      121 ccgcctgcgc atggtgacag ccatgtttct gaccaagcat ctgtttatcc actggaagga
      181 aggcgagcgc tggttcatgc agcagctgat cgacggagat tttgccgcca acaacggtgg
      241 ctggcagtgg agcgcgtcca ccggcgccga cgctgcgccc tattttcggg tgttcaatcc
      301 tacccgtcag tcggaacgct tcgatgccga cggtacgttc atccgtcact ggctgccgga
      361 actgaatacc ctcggcggca aacagattca cgatccgacg cctctggaac ggcaactgtg
      421 cagctacccg catcccatcg tggatcaccg cgtggccgtg gaccggatca agcaggaatt
      481 ccagtcgctc aagaccgtgg cattggcgta aacccgaacc agaacaaatg gagaactcat
      541 gaagagcatc agcaatggca tccgcctctg gtttgattcg gaagcaaccg aggtgggcaa
      601 cagtgagcaa gtgaactggt tgcgctgcat tcccttcgtg ctgctgcacc tggcgtgtct
      661 cgccgcattc tggaccggcg tgagtacgtt tgccatcctg ttctgtgttg catttttctg
      721 gctgcgcatg tttgcgatta cggcgttcta tcaccgctat ttttcccacc gcagcttcaa
      781 aacatcccgc accgcacagt tcctgtttgc cgtgctgggt aacatgtcgg cccagcgtgg
      841 cgccctgtgg tgggccgggc atcaccgcca tcaccaccag cattccgatc aggcgctgga
      901 cctgcattca ccggtgcagc gcagtttctg gtggtcgcac atgggctggt tcacctgcga
      961 cgcctccttc aaaacaccgt acgaccgcat ccgtgatttc gcgcgttttc cggaactggt
     1021 attcctcaat cgctacgacg cactggtgcc ggcactgacc atgctggcga ttttcagcct
     1081 gggtgagctg ctggccgcat tcgcgccggg tctggaaacc agcggtctgc aattgctggt
     1141 gtggggattt ttcatttcca ccacactgct gttccattcc actgtcacca tcaactcact
     1201 ggcccatgtg tggggcaagc gccggttcgc aaccggcgac aacagccgca acaacctggc
     1261 gctggccctg attaccctgg gcgaagggtg gcacaacaac caccaccgct atccggcgtc
     1321 ggcgcggcac ggctttgcac gctgggaaat cgacatcact tatttgctgc tgttgctgct
     1381 ggaaaaagtg gggttgatct gggaactgaa gccgattccc gcgcgcatcc ggcaggaaat
     1441 gcagtccggc gcagcatcag tgcgctgaac tgatttgcat gaaagacaat tgtgtgagaa
     1501 ctgaaatcat ggaatacaaa aagatcgccg tcgttggcag cggcatatcg ggactcctga
     1561 gtgcactgtt gctgcaaaaa aagtacagcg tatccctgtt tgaacaaaac gcttacctgg
     1621 gtggccacac gcacaccctc gacgtcgatc tggatgacca gcgctttccg gtgaataccg
     1681 gctttatcgt ctataacgac tggacctatc cgaatttcat ccggctgatg gatcacctgg
     1741 gtgtagccag cgaagcgtcc agcatgagct tcagcgtacg ctgcgaccga tccgatctgg
     1801 aatataacgg cacctctttc aacagtctgt tctgtcagcg caagaatctg gtgagtcgcc
     1861 gcttctggcg catggtgagt gatatcgtgc gtttcaaccg gcaagccacc cgtgcccatc
     1921 agcagggcga catcgatcct ggcatgaccc tgcagcgctt tctggatgaa aaccgctaca
     1981 gcgaggaatt catacgctat tacatcatcc cgatgggcgc ggcaatctgg tcggcgccgg
     2041 agtccgacat gctgggattt ccgctcgcct tttttgtgcg cttctttcac aaccacggca
     2101 tgctgtcagt ggaccagcgc ccccaatggc gcgtcatttc cggcgggtcc cgcagttacg
     2161 tcaatgccct gaaacagcaa ttgcgcggac ccacgcatat cgggcgcaga gtcgagcaga
     2221 tcgaacgttt ctcgcatggc gtgttgctgc gcgacgatca gggccagctg gaacagttcg
     2281 acgcagtggt gctggcctgc cacagcgacc aggcgctggc gatgctgacg cagccttcac
     2341 cggaagaacg ccagtttctg ggcgccattc cctaccagga caacgatgtg gtgctgcaca
     2401 ccgacattcg cctgctgccg caacgcaaac tggcgtgggc cgcgtggaac taccgcatcc
     2461 cggaacgggc tgatcgtccg gtccacgtca cctacaacat gaacattctg cagaattttg
     2521 tcagcgatac cactttctgc gtcagtctga accagaacga ccgcatcgat ccggcacgca
     2581 tcattgcccg ctaccgctac agccaccccg cctacacgct ggcgggcatg catgcgcaaa
     2641 acagtttcga ccggatcaat ggcaaacacc atacctatta ctgcggcgcc tactggttca
     2701 acggttttca cgaagacggc gtcaacagcg ccattcgtgc cgcccgctct ctgggcgttg
     2761 atttcgacga ggtgattgcg ccatgcaaag cgccctctac gaaggcgtcg tgagccaccg
     2821 gcgacaaacg ccgactgcgc acgaattcag gtatcccctc ttcatgctgt acctgaatct
     2881 ggacgaactg gaggactttt tcgcgctgtc gccgctgtgg tcgctggagc gtttcaactg
     2941 ggccagcttt taccgcaggg attttctgaa tccctccgtg gccagcctgc gcgacgcggt
     3001 acggctggaa atccggcagc agaccggcca cacgttcgac ggcgatatct ttctgctgac
     3061 ccacatccgt taccttggat actgcttcaa cccggtgtcc ttctactatt gtttcgagca
     3121 aggcaagctg acctggattg tagcggaaat caacaacacg ccctggaacg agcgcttccg
     3181 ttatgtgctg cagtgcagcg ctgaagaaaa caggcagtcc tttgcattcg acaaggcgtt
     3241 tcatgtttcc ccgtttctgc ccatgggcat gcaatatgaa tggcagttcg gcctgcccgg
     3301 cgaaaagctg tcggtattca tgcgcaaccg gcaggacggc aacgaggttt tcaacgccac
     3361 cctgctgctg cagcggcagg acgccaccgc gtcagcactg aaccggatac tgttgaaatt
     3421 tcctgccgtc accatcaaga cggtcgccgg catttactgg caggcgctaa agctctggct
     3481 gaagcgcacg ccaatttacg atcacccctt gtcagatgag gcaactcccc atgcaatcct
     3541 caaccgtcag caaagcaaca ccactgacca gcactgagcg tttttgccgc cagctgttgc
     3601 tgaaagcgtt cgcccaggcg gaattcggta ccctcgtgat ctgcgatccg gatggcgaat
     3661 acgaatgccg tggcagcaag gacgccagcg cgattcaggc ccgtatcgat gtgcaggatt
     3721 ccgcctgtta ccgcgacatg gtgttgggtg gcagcattgg cgcagccgaa gcctacatga
     3781 ccggcgactg gacctcgccc gatctcactg cggtgatccg cttcatgtcg cgcaatctga
     3841 acgcgctcaa ccggatggag agcggactgg cccggctggc acaacccttc cagcggctgt
     3901 atcactggaa caaccgcaac accgaaaagg ggtcgcgtcg caatattgcc gcgcactacg
     3961 atctgggcaa cgatctgttc cagctgttcc tcgaccccac catgatgtac tccgccggca
     4021 ttttcccaag tccatatgca acgatggagc aggcgtcggt caccaagctg gaccggatct
     4081 gccagaagct ggacctgcaa ccgcacgacc atgtgatcga gatcggcacc ggctggggtg
     4141 gctttgccat tcacgccgcc acccactatg gctgcaaggt caccaccacc acgctgtcgc
     4201 aacagcagca cgattatgcc cgcaatgaaa tccgtgcgcg gggactggag gacaacatca
     4261 cgttgctgct gcaggattac cgcgacctga agggcgagta tgacaagctg gtgtccatcg
     4321 aaatggtgga agcggtgggt caccagtact acggcgccta tttcgaaacg ctgggccggc
     4381 tgctgaagcc caacggcatc ggattgatcc aggcgattat catcgatcac aagcgctacc
     4441 agactgccat caaaaacgtc gactttatcc agcgctatat ttttccgggc tcctgcatcc
     4501 cgtcagtgaa tgcgttgctg cagaactgta gcgatcacag cgatctgcaa ctcacccatt
     4561 tcgaagacat caccccccac tatgcccgca cgctgcgcga gtggcgtcat gcttttttcc
     4621 gtaatctgga caagatcaag gcactggggt acagcgacga attcgtccgc atgtgggatt
     4681 tttatttctg ctattgcgaa ggcggcttcg aggaacgggt aatcggcgac gtgcaattgc
     4741 tgttcgccaa acctggctat cgtggccagc ccgtgctggg acgtctttga gatgttgctg
     4801 aacgccgtgc tgttccaggc aggatggttg ctgtgcgtga tggagcgtgg gccgctggcc
     4861 ctgctggcgt cggcactcat cctgctagtg cacggacggt atgtgaccag gaaccggcgc
     4921 gaatggctgt tcatcctggc gctgggcagc gtcggcattg cgctggattt tctgttcatg
     4981 aagtccggtg tctttcacat cgacggccat gattttccgc cgccctggct gatgctggtg
     5041 tggctgctgt tcgccagtac gctcaatcac tccctgcgct ggctggatca gcgactctgg
     5101 ctggcagccg ttctgggtgc cattgccgcg ccactgagct atctggctgg cgcgcggctg
     5161 ggtgcgctgc agatcgaact gcacatgttg ccggtgctgg gcgccgcctg ggctgcgctg
     5221 ctgccgctcg cctttctgtg caatcgccgt ctgctgctaa cacagccacc gacgcagacg
     5281 accgggagca caacaccttg aaccgttcgc acgccacgct gctaacgctt gttgtcatca
     5341 tcacattccc gctggcgcac gcccggcagg attcaccgtc accgcaaacc gtatcggcca
     5401 aacagctgca ctatgtcggc actgccacgc gactggacac tgtcattccg gcctacaccg
     5461 aatcctacac cgagcactat gatgcgcagg gcaatctgct gagcggtcag gtgcgatacc
     5521 aggatgcaca gggtgaaccc ctcgccgaca aacagctgga ttatcgccct catccccacg
     5581 caccggcttt cagctttcac aatcgcaaaa ccggctatcg tgaagcggtg atctggcggg
     5641 acgatggccg cattcttctg atgaatgccg acgcggccca tacactgcca cgggaaaaaa
     5701 tcctccgcgt gccggagccc gtggtggcag acgccggctt caaccgtttc gtgcaggaca
     5761 atcttgcacg gctggaacag ggtgagcgga tcgaattcca tttcctgaat cccgcccggc
     5821 tcgactggtt tcgtttcagt gccagcggca atgtgcttga tcagcagaac atgcaggtgg
     5881 tgatagcacc cgccaatccg gtgctgcaat ggctggtgga accgatccgg ctggtctatg
     5941 cgcgggattc gggacgtctg ctgcagtacg aaggcattac caacatcagc ctggacggcg
     6001 gcgacaccct ggtggcccgc atccagtacc gctattccat gaaaccggta gaatatccgg
     6061 acactttacc cgagggctaa cgcgtgcatt ccattcagtc agttgcggaa cggacagggc
     6121 tgacaccgga tgtcatccgc atctgggagc ggcgctatca ggccgtggca ccagaccgca
     6181 cggcgtcgaa ccagcgcctg tacagtgacg acgacgtaga acggctggca ctgctgaaac
     6241 gggttaccga caacggccgt cgcatcagca tcgtggcgaa tctcagcaac gacgaactgc
     6301 gccagatggc ggcccaggtg caagtcgcac cacgcaccgc cctccccgcc gtccaggttg
     6361 atccgctcgc tctgcaggaa caggcgctgg acgccattca gcaactggac ccggcgctgt
     6421 tcgatcaatt gctggaacgg gcgatggtgg gcatgggatc ggtgggcttc atgatgcggt
     6481 tcgccgcacc gctgatggtg acggtaggca acctgtggcg ttccggcgcc gtgcgcacct
     6541 gccaggaaca tttcgccagc gcgcacctgc gttccttcct gggccgctac atgctggatg
     6601 ccaacaccga tccgctggga ccgcgcatcg tcatcgccac cctgccgggc caggctcacg
     6661 aactgggcgc caccatggcc gccgtggtgg cggcgcaatc cggctggaat gtgctgtacc
     6721 tgggcgcgaa tgtacccatc gaggaaatcg ccttcgccgc cgactgcaag gatgcccgcg
     6781 ccgtggccgt cagcctgagt tatccgctgg atgatccccg cgtgccggtg ctgctgaaag
     6841 aactgcggca gaaattgcca tcgtcatcaa cgttgatcgt cggcggtatt ggctctaccc
     6901 ggcaacaacc attgctggat gagttgaaag cgctgcaggc cgacgatctg gtgcgactgg
     6961 cagaacatct ggaccggatg cgctgagagg ctgcgtcact gacgtcgcag ccttccatgc
     7021 tttcggcatt tatttggttc ccatcaaccc ccggaccgta ttcagcatat ctgccggcag
     7081 caccagcgtt ttcgtattgg gcgacgccgc cagatccgtc agcgcgttca cgtatttctc
     7141 gcccagcaga taggtcaccg gcaatgaatc acccttgatc gactccgtca ccagcgtgat
     7201 ggcatcgcgg ctggcattgg ccagcaccac ctgggattcg gcgtccttgc ggctggcttc
     7261 caggcgccct tccgcctgca ggatcgcagc ctgcttttca ccttcagcct tggtcactgc
     7321 cgcgcgccgc tgccgttctg cggccgcctg ttcctccatt gcctgctgca tggtttccga
     7381 cggtttgatg tcctggattt cc
//
LOCUS       DASUEG010000160         6049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_114235, whole genome shotgun sequence.
ACCESSION   DASUEG010000160 DASUEG010000000
VERSION     DASUEG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114235"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFX11_03095"
     CDS             <1..557
                     /locus_tag="VFX11_03095"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937631.1"
                     /translation="DAIVLLLTPPFTDDAQPRPGRITQYPPGVRENGAQYSHGSSWLV
                     DAALHLADMLEQQGNAAAAQHWRTRAGTLWHKISPLTHTTPERWLNYGLEPHQQPADV
                     YFGPGYEGRGGWSWYTGSAARMLTAAWGLLGLEFRAGELHVADWAKRGDVWPHLQQVE
                     WRGHVVEVTAAQAGATGSAPPASG"
     gene            complement(505..921)
                     /locus_tag="VFX11_03100"
     CDS             complement(505..921)
                     /locus_tag="VFX11_03100"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX4937632.1"
                     /translation="MGSYIVLAADQARARLFKAEKREGALVEITGMANPEARLREQDL
                     VSDASGGNPHDIGNHDSAHQHVAEVFAKQVGQALEQALQAHKPDRIYVLASPSFLGLL
                     RPHWSTALKGKIAQEFAKDVSRMPAEQIRSHLPELL"
     gene            1131..3998
                     /locus_tag="VFX11_03105"
     CDS             1131..3998
                     /locus_tag="VFX11_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HEX4937633.1"
                     /translation="MSQPKEIAPDLQDGIDRKQLKQLKERFLQVNHGRLERMLSTLGS
                     RHRLFLESLPLLLHINHPSLPGYVSGRTPSGVCGFTPEKENLRAAQTISRSFVYRRKV
                     NHQPRIFSIFLMGSTGTVAHSESSDMDIWICYDPGIPQEAIDELQDKLSAIERWADET
                     GLEVHFFLMDPIKFRTGVRSDLSGEDCGSAQHYLLLDEFYRTSIMVAGRYPLWWMVPA
                     YNEKNYDALADKLVRNRFIEKDDCVDFGGISDFPAGEFIGAGMWQLYKGIDSPYKSVI
                     KIVLTEVYAGEYPEVESLSVMYKKAVYAGQLDLDELDPYVMLYRKIERYLSARQELRR
                     LELIRHCFYFKVNESLSRAVKAADCSWRRHLMIRLTREWGWDDGYIKQLDNRKHWKVT
                     RVRAERQELVQELNQSYRVLSAFARDNDIEASIKQEDMNLLGRKLYAAFERKGGKIEL
                     VNPGIAASLVEEHLSFHYVRDTEHGINDYWAVYQGYLKMREAKGREPVKKARSIIELM
                     TWSYFNGLLDASTRMSAEANGTDMTDAELQQTVQSLFQLFPGGAPYAAQENFLHAAIP
                     TRIVLYLNAGLDPMRPMTQKGIHRLTDQTDALSYSALHHNLVVTVDQILCNSWGEIIC
                     SHFSGENALIDCLVHYLRQIPPDGSIPLPKLDVRCYCPTRASSIATRVEELFRDIIAC
                     YYSGTRTIHTRYILEIEQYSYLLQFKRNVPVVRGLRSRRELLDVLSEPQQTYSPIVLD
                     RNALDKHPLRVISKLASPDRVQVFYLRNNDSSEIYIHDELGSLFCTQKNNVDEETLLN
                     PIRLFLQNIQHRRTTVNQRDGYSTPQPQLQKVAYYELKRNNRGEFQSERKPLLEVVDS
                     KQFHHVQAIAQTGTFGDVFYTVYCDDQEFSQLEYGDALFAAVAGHIASLRRKRERYPI
                     YITDLDLSQLDLAGGAPLQTVQFLQHKEKLETAINQALKSS"
     gene            4083..4385
                     /locus_tag="VFX11_03110"
     CDS             4083..4385
                     /locus_tag="VFX11_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX4937634.1"
                     /translation="MRIPLLIGLLALTAACGQKGDLYLPEDDPNAPGAVVEQTCRSRT
                     CKPVETAPASEATAEPAASTPATAPAGNEAAAPSPEQPATITLEPKPQAPSEETTQ"
     gene            4382..5629
                     /gene="lysA"
                     /locus_tag="VFX11_03115"
     CDS             4382..5629
                     /gene="lysA"
                     /locus_tag="VFX11_03115"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX4937635.1"
                     /translation="MSQFEYRNNRLHAEQVEAQAIAAAVGTPCYVYSRAALESHWRAF
                     DSAFGSHPHLICYAVKANSNLAVLNVLAQLGAGFDIVSQGELERVLRAGGDPAKIVFS
                     GVGKLRGEMVRALEVGVHCFNVESEAELDTLNDVAGSLGKIAHISLRVNPDVDAQTHP
                     YISTGLKQNKFGIDIDRSLAVYQHAATLPHLKITGIDCHIGSQLTSVTPFLDALDKVL
                     ERIDQLAAHGIHIRHLDIGGGLGVRYRDEQPPTPQTYVTALLHKLGDRDLKILMEPGR
                     AIAANAGILLTEVLLLKSTEVKNFAVVDAAMNDLIRPSLYGAWQNIIPLQPNEKAQKQ
                     TYDIVGPVCETGDFLGKDRELAIAPGDLLAVCSAGAYGFVMSSNYNTRNRAAEVMVDG
                     DHFHIVRRRETYDDQLALESLVS"
     gene            5634..>6049
                     /gene="dapF"
                     /locus_tag="VFX11_03120"
     CDS             5634..>6049
                     /gene="dapF"
                     /locus_tag="VFX11_03120"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX4937636.1"
                     /translation="MKIEFTKMHGLGNDFMVINQVTQNVELTPDMIQRWADRHTGVGF
                     DQLLLVSPPTSPDVDFRYRIFNADGTEVEQCGNGARCFARFVYDKNLTNKNVIPVETN
                     TGRIELQLVSGGLVTVNMGAPIFEPERIPLIAPQQQP"
BASE COUNT         1308 a         1974 c         1722 g         1045 t
ORIGIN      
        1 gggacgccat cgtgctgctg ctgacaccgc cgttcaccga cgacgcgcaa ccgcgccccg
       61 gccgcatcac gcaatatccg ccgggcgtgc gcgaaaacgg cgcccagtat tcccacggca
      121 gttcctggct ggtcgacgcc gcactgcacc tggccgacat gctggaacaa cagggcaacg
      181 ccgctgccgc acagcactgg cgcacccgcg ccggcaccct gtggcacaag atttcacccc
      241 tcacccacac gacgccggaa cgctggctca actacggcct ggaaccacac cagcaaccgg
      301 ccgacgtgta tttcggcccg ggctatgagg gccgtggcgg ctggagctgg tacaccgggt
      361 cagccgcgcg catgctgaca gcagcgtggg gattactggg actggaattc cgcgccggcg
      421 aactgcacgt ggccgactgg gcgaaacggg gggatgtctg gccgcacctg caacaggtgg
      481 aatggcgcgg ccatgtggtg gaggttacag cagctcaggc aggtgcgacc ggatctgctc
      541 cgccggcatc cggctgacat ccttggcaaa ctcctgcgcg attttgcctt tcagcgcggt
      601 gctccagtgg gggcgcagca ggcccagaaa ggacggactg gccagcacat agatgcggtc
      661 cggcttgtga gcctgcagcg cctgctccaa tgcctgtccc acctgcttgg cgaacacctc
      721 tgccacatgc tggtgagccg aatcgtgatt gccaatgtcg tggggattgc cgccactggc
      781 gtcactcacc agatcctgtt cccgcaaccg cgcctcgggg ttcgccattc cggtgatttc
      841 caccagcgcc ccctcgcgct tctcggcctt gaacagacgg gcacgggcct gatcggccgc
      901 cagaacgatg tacgatccca tggttagcat ccttttgctg gtaggtagac agaaacgact
      961 ggccccagca tacacccgtg ccggtggcgg cctgccttga taggggtcaa acattccccc
     1021 attgacctgg cccgcacggc acccaactca ccaaccccct gaaattccgg cgaaaaatcc
     1081 cggggctgcg aaggcgggca aaattcacca cccgccctat acttggatgg atgtctcagc
     1141 ccaaagaaat tgcacccgat ctgcaggacg ggattgaccg caagcagctg aagcagctca
     1201 aagagcgctt cctgcaggtc aatcacggca ggctggagcg tatgctatcc acgctcggca
     1261 gccgccaccg gttgttcctg gaatcgttgc cgttactact gcatatcaac cacccgtcac
     1321 tgcccggcta tgtctctgga cgcacgccca gcggtgtgtg tggtttcacc cccgaaaaag
     1381 aaaatctgcg ggcggcccag accatctccc gcagcttcgt ctaccggcgc aaggtgaatc
     1441 accagccgcg catcttcagc atttttctga tgggcagcac cggcaccgtg gcacattcgg
     1501 aatccagcga catggatatc tggatctgct acgacccggg cattccccag gaagccatcg
     1561 acgaattgca ggacaagctc agcgccatcg aacgctgggc cgacgagacc ggactggaag
     1621 tgcatttctt cctgatggac ccgatcaagt tccgcaccgg cgtgcgcagc gatctgtccg
     1681 gcgaggattg cggcagcgcg cagcattacc tgctgctgga cgagttttac cgcaccagca
     1741 tcatggtggc ggggcgctat ccgctgtggt ggatggtgcc ggcgtacaac gaaaaaaatt
     1801 acgacgcact ggcagacaag ctggtgcgca accgcttcat cgaaaaagac gactgtgtgg
     1861 atttcggcgg catctccgat tttcccgccg gcgaattcat cggcgccggc atgtggcagc
     1921 tgtacaaggg aatcgattcc ccttacaaat ccgtcatcaa gatcgtgctc accgaagtgt
     1981 acgccggcga gtatcccgag gtggaaagcc tgagcgtgat gtacaaaaaa gcagtctacg
     2041 ccggccagct ggatctggat gagctggacc cttacgtgat gctgtaccgc aagatcgaac
     2101 gctacctcag cgcacgccag gaattgcggc ggctggaact gatccgtcac tgtttctatt
     2161 tcaaggtgaa cgaaagcctg agccgcgcgg tgaaagcagc ggactgctcc tggcgccggc
     2221 acctgatgat ccggctgacc cgcgaatggg gctgggatga cggctacatc aagcagctgg
     2281 ataaccgcaa acactggaaa gtgacccggg tacgcgcgga acggcaggag ctggtgcagg
     2341 aactcaacca gagttaccgc gtgctgtcgg cgttcgcccg cgacaacgac atcgaggcct
     2401 cgatcaagca ggaggacatg aacctgctgg ggcgaaagct gtatgcagcc ttcgaacgca
     2461 agggcggcaa gattgaactg gtgaatccgg gcatcgctgc ctcgctggtg gaagaacacc
     2521 tgtcatttca ctacgtgcgc gacaccgagc acggcatcaa cgactactgg gcggtgtacc
     2581 agggctacct gaaaatgcgc gaggccaagg gccgcgaacc ggtcaagaag gcacgcagca
     2641 tcatcgagct gatgacctgg agctatttca acggcctgct ggacgccagc acccgcatgt
     2701 cggcggaagc caacggcacc gacatgaccg atgccgaact gcaacagacc gtgcaatcgc
     2761 tgtttcagct gttccctggt ggcgcgccct atgccgccca ggaaaatttc ctgcacgcgg
     2821 caattcccac ccgcatcgtg ctgtacctga atgccggtct cgatccgatg cggccgatga
     2881 cgcagaaagg catccaccgc ctcaccgacc agaccgatgc gctcagctac agcgcgctgc
     2941 accacaatct ggtggtgacg gtggaccaga tcctctgcaa cagctggggg gaaatcatct
     3001 gcagccattt ttccggcgag aacgcgctga tcgactgtct ggtgcactat ctgcggcaga
     3061 tcccgccgga tggctcgatt ccgttgccga agctcgacgt gcgctgctat tgccccaccc
     3121 gcgccagctc aatcgccacc cgggtggagg aactgttccg cgacatcatt gcctgctact
     3181 acagcggcac ccgcaccatt cacacccgct acattctgga aatcgaacag tacagttacc
     3241 tgctgcaatt caagcgcaat gtgccggtgg tgcgcggcct gcgttcacgt cgcgaactgc
     3301 tcgacgtgct gagcgaaccg caacagacct acagcccgat cgtgctggac cgtaacgcgc
     3361 tcgacaaaca tccgctgcgg gtgatcagca agctggcctc tcccgaccgc gtgcaggtgt
     3421 tctacctgcg caacaacgac agcagtgaaa tctacattca tgacgagctc ggctcgctgt
     3481 tctgtaccca gaagaacaat gtcgacgagg aaaccctgct caatccgatc cgcctgttcc
     3541 tgcagaatat ccagcatcgc cgcaccacgg tgaaccagcg cgacgggtat tccacgccac
     3601 aaccgcagct gcaaaaggtg gcgtactacg aactcaagcg caacaatcgc ggcgaatttc
     3661 agagcgagcg aaaacctttg ctggaagtgg tggattccaa acagttccac cacgtacagg
     3721 ccatcgccca gaccggcacg ttcggcgacg tgttctacac cgtatattgc gatgaccagg
     3781 aattttccca gctggaatac ggcgacgccc tgtttgccgc cgtggccggc catatcgcct
     3841 cgctgcgacg caagcgcgag cgctatccca tctacatcac cgacctggac ctgagccagc
     3901 tggacctggc cggcggcgcc ccgctgcaga ccgtgcagtt cctgcaacac aaggaaaaac
     3961 tggaaaccgc catcaaccag gcgttgaagt cgtcctgaac ggaaagccgc ctgaacggaa
     4021 agcctcctga tttggcggcc gcttggttat aatggccagt ccccgctcac ccggaatatt
     4081 ccatgcgcat cccattactg atcggcctgc tggcgcttac tgccgcctgc ggccagaaag
     4141 gcgatctcta cctgccggaa gacgacccca atgccccagg tgcagtggtg gaacaaacct
     4201 gccgcagccg tacctgcaag cccgtggaaa cagctccggc cagcgaagcc acggcggaac
     4261 ccgcggcgtc cacgccagcc actgcgccag ccggcaacga agcggctgcc ccatccccgg
     4321 agcagccagc gaccattacg ctggaaccca aaccccaagc gccctctgaa gagaccaccc
     4381 aatgagccag tttgaatacc gcaacaaccg cctgcacgcc gaacaggtcg aggcgcaggc
     4441 catcgccgcc gccgtcggca ccccctgcta cgtctattcc cgcgcggcgc tggaaagcca
     4501 ctggcgcgcg ttcgattccg ccttcggtag ccacccccac ctgatctgct atgccgtaaa
     4561 agccaattcc aatctggcgg tgctgaacgt gctggcccaa ctgggcgccg gcttcgacat
     4621 cgtgtcccag ggcgaactgg aacgggtact gcgggcgggc ggggatcccg ccaaaatcgt
     4681 gttctccggc gtgggcaagc tgcgcgggga aatggtgcga gcactggaag tcggcgtgca
     4741 ctgcttcaac gtggaatcgg aagcggaact ggataccctg aacgacgtgg caggatcgct
     4801 gggcaagatt gcccatatct ccctgcgggt caatccggac gtggacgccc agacccaccc
     4861 ctacatttcc accggcctga aacagaacaa gttcggcatc gatatcgacc gcagcctggc
     4921 ggtgtaccag cacgcggcaa cgttgccgca tctgaaaatc accggcatcg actgccacat
     4981 cggctcgcag ctcaccagtg tcacgccgtt tctggacgca ctggacaagg tactggaacg
     5041 catcgaccag ctcgccgccc acggcatcca catccgtcat ctggatatcg gcggcggcct
     5101 gggtgtgcgc tatcgcgacg aacagccacc cacgccgcaa acctacgtca ccgcactgct
     5161 gcacaaactg ggtgaccgcg acctgaaaat cctgatggaa ccgggccgcg ccattgcggc
     5221 aaacgcgggc atcctgctca ccgaagtgct gctgctgaaa tccaccgaag tgaaaaactt
     5281 cgccgtggtg gacgccgcca tgaacgactt gatccggcct tccctgtacg gtgcctggca
     5341 gaacatcatt ccgctgcaac cgaatgaaaa agcgcaaaaa cagacctacg acattgtggg
     5401 ccccgtgtgc gaaaccggcg atttcctcgg caaggaccgc gaactggcca tcgcgccggg
     5461 cgacctgctg gcggtctgtt ccgccggcgc ctacggcttt gtgatgagtt ccaactataa
     5521 tacccgtaac cgcgccgccg aagtgatggt ggatggcgac cacttccaca tcgtgcgccg
     5581 ccgcgaaacc tacgacgacc aactggccct ggaatcactg gtatcctgag acgatgaaaa
     5641 tcgaattcac caaaatgcac ggactgggca acgatttcat ggtcatcaac caggtgacgc
     5701 agaacgtgga attgaccccc gacatgattc agcgctgggc cgaccgccac accggcgtcg
     5761 gcttcgacca gttgctgctg gtgtcgccgc ccacctcgcc cgacgtggat ttccgctacc
     5821 gcatattcaa cgccgacggc actgaagtgg aacaatgcgg caatggcgct cgctgttttg
     5881 cccgcttcgt ctatgataag aatctcacca acaagaacgt gattccggtg gaaaccaaca
     5941 ccggccgcat cgaattgcaa ctggtgagcg gcggactggt caccgtgaac atgggtgccc
     6001 cgattttcga accggaacgg attcccctga tcgcgccaca gcaacaacc
//
LOCUS       DASUEG010000161         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1707596, whole genome shotgun sequence.
ACCESSION   DASUEG010000161 DASUEG010000000
VERSION     DASUEG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707596"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFX11_03125"
     CDS             <1..481
                     /locus_tag="VFX11_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937637.1"
                     /translation="VEYITSKPMNLDKLREYGPVVELGRLSVAEDYRMAPEVLAGLIM
                     CATETALAHDTCFIVLQAVEKTARIYSKMGFAPILEESVTNLEYGTPMKLFALNLAAQ
                     SGTRNPYRDNMAHHFSPYIVHRFVWRQIVKTLLRGRSRQKRISDLSTQELPALAQFT"
     gene            783..1580
                     /locus_tag="VFX11_03130"
     CDS             783..1580
                     /locus_tag="VFX11_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4937638.1"
                     /translation="MNTPLASASQKTIFITGATAGIGRATARLFAEKGWYVGLAARSA
                     GKLQELQKELGAANCSGHVMDVTDAGQVNQALQAFGENTGGRLHVLVNNAGVLHAGDF
                     EDMSTHQHHSILDTNIKGVIHCMQGAFPLLKNTPDSHVINMSSASAMYGSPTLATYSA
                     SKFAVRALTEALNVEWARHDITVTDIMPPFVDTDMLDNPVRSRIKAISLLGVQLSAED
                     VAQSVWNAIASRQLHDRVTPSFRLLALMQKHAPDRIQRRFVKFLSGY"
     gene            complement(1592..>2038)
                     /locus_tag="VFX11_03135"
     CDS             complement(1592..>2038)
                     /locus_tag="VFX11_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937639.1"
                     /translation="YWYSEPMPTPATSAMWLVVNPVMPSFWRSRVRCCLPCAPGGGEP
                     FGIRVGVIEPEAHRTGISMQQPALPLREYDAARRQVGNGIEEQIRTGPDPLNIARIAH
                     GMAVSASAPYRRLAGRRAFLVGLLRRFASDAALHWISRRAFGLGKG"
BASE COUNT          439 a          609 c          606 g          384 t
ORIGIN      
        1 ggtggaatac attacatcaa agccaatgaa cctggacaaa ttgcgcgaat acggcccggt
       61 ggtggaactc ggacgtctga gcgtggcgga agattaccgc atggctccgg aagtgctggc
      121 aggtctgatc atgtgtgcca ccgaaacggc gctggcacac gatacctgtt tcatcgtgct
      181 gcaggccgtg gagaagacgg cccgcatcta ttccaagatg ggattcgcgc ccattctgga
      241 agaatcggtg accaatctgg aatacggtac gccgatgaaa ctgtttgctc tcaacctggc
      301 ggcccagtcg ggcacccgca acccgtaccg ggacaacatg gcccaccatt tttcgccgta
      361 cattgtgcat cgcttcgtgt ggcgtcagat tgtgaaaacc ctgttgcgtg gccggagcag
      421 gcagaagcgg atttcggatc tgagtaccca ggaactgcca gcactggcac agttcacgta
      481 aggtgaatca gtcgggggaa acgcgcgcca gcgcgcgcat atccctgcat gcaattccct
      541 cccgcacgtt gtacctggca gcagccatgg ttgcagtcgg cacgagccaa cagccacgag
      601 atcagggacc gagtctgccg cagcatgttt tcaatccctt ggcaagcgca ggaacactcg
      661 gcatacaacg tacgtaacgg gcaatgacct gacatcgagc aaggtagaca atatctcccc
      721 cgtcggttta tcagcggaac cacagtacct attcgacttg cggtcattca agggagccat
      781 tgatgaatac cccacttgcc tctgcgtcgc agaaaaccat ctttatcaca ggcgccaccg
      841 ccggtatcgg cagggctact gcgcgcctgt ttgccgaaaa aggctggtac gtgggacttg
      901 ccgcccgcag tgcgggcaag ctgcaagaac tgcaaaaaga actgggcgcc gcgaactgca
      961 gtgggcatgt gatggatgtc accgatgccg gacaggtaaa ccaggctctg caggcgtttg
     1021 gagaaaatac cggaggccgt ctgcatgtac tggtgaacaa tgctggcgtg ttgcatgccg
     1081 gtgacttcga agacatgtcc acgcaccagc accacagcat cctcgatacc aatatcaaag
     1141 gcgtgattca ttgcatgcag ggcgcctttc cgctgctgaa aaacactccg gattcgcacg
     1201 tcatcaacat gagctcggca tcggccatgt atggatcgcc gacgctggcc acctattccg
     1261 ccagcaagtt tgccgtgcgg gccctgaccg aggcgctgaa tgtggaatgg gcgcggcatg
     1321 acatcactgt gacggacatc atgccgccgt ttgtggatac ggacatgctg gataatccgg
     1381 tgcgcagccg catcaaggcg atctcgctgc tgggcgtgca gctgtcggcg gaggatgtgg
     1441 cgcagtcggt atggaatgcg atcgcatcgc gccagttgca cgaccgggtg acgccctcat
     1501 tccggctact ggcgctgatg cagaagcatg cgccggaccg catccagcgc cggtttgtga
     1561 aattcctgtc gggatattga aagcgctttc atcaaccttt tcccaaacca aatgccctgc
     1621 ggctgatcca gtgcaacgct gcatcactgg cgaaacgccg cagcagcccc accagaaagg
     1681 cgcgccttcc cgccaggcga cgatagggag ctgacgccga aaccgccatg ccgtgcgcaa
     1741 tgcgggcgat gttgagcggg tccggcccgg tgcggatctg ctcttcaatc ccgttgccca
     1801 cctgccgacg cgctgcatcg tattcccgca acggcagggc cggctgctgc atgctgatgc
     1861 cggtgcgatg ggcttccggc tcgatcacgc caacgcggat gccaaagggt tccccgccgc
     1921 cgggcgcgca tggtaggcag caccgcacgc gtgaccgcca gaaggatggc atcaccgggt
     1981 ttaccaccaa ccacatcgcg gacgtggccg gcgtgggcat cggttcgctg taccagta
//
LOCUS       DASUEG010000162         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1143732, whole genome shotgun sequence.
ACCESSION   DASUEG010000162 DASUEG010000000
VERSION     DASUEG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143732"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1395
                     /locus_tag="VFX11_03140"
     CDS             46..1395
                     /locus_tag="VFX11_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019839235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEX4937640.1"
                     /translation="MQSRQAYLDTLKTLSDRLIAIQQPIRILDSIKWPAQWRQDFFKS
                     GMKTLPPADSTYYQSIPLNFNFDAKQAELQDLRKDIVRKLGRKDGLGRILLATTEQYL
                     KVIDLLRARGTPAFGGFSKDLYGSAHDHLSGDKRTLREVGEQLCSIFSHPAAQHIAFS
                     QPKKYDAEAAVRILRHRLASYFPDGELQVKLSDGIVSDAAAGGDSIKMNARSMFSERD
                     LQVLEVHEGWVHVGTTLNGRRQPYASWLSVGSPRIAAIQEGLALLMETLTFSSFPHRA
                     RRVSDRVAAIHLAEEGADFIQVFRHLADRGVSEPDAFTLTQRVFRGGLCDGGAAFTKD
                     LAYVKGFVENVNFMRSAIAAGLPELIPMLFVGKITLDDIPVLYERYLEGMIEPPKYLP
                     AMFRDLNGLYVWFGFSNGMGMVDIKRVQKHFLKNFAQIRPVCPIEMAPRAPVSTEPE"
     gene            1486..2253
                     /locus_tag="VFX11_03145"
     CDS             1486..2253
                     /locus_tag="VFX11_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4937641.1"
                     /translation="METLPNSGLHNFPSAHHCHTCPLQSRCVPSSLNLTDLEQFDHII
                     HRSRPLRRGEHLYMAADPFRSIYMILQGTIKTYCLTEDGDEQVTGFLTTGDCFGFDGL
                     NIMKHTNSAKALERTQVCEIPFERLEQLCRRIPSLQHYAYQLASRQILQEQKLLQFLG
                     KRSAEARVAYLLLNFIGRVNHDHPLTRPIQLPMTRYDTANYLGLSVETVSRILSRMQQ
                     LGVIRLRGRLVAIQSPAALTECAQVETNVKPVRVMSL"
     gene            2388..>3702
                     /gene="pyk"
                     /locus_tag="VFX11_03150"
     CDS             2388..>3702
                     /gene="pyk"
                     /locus_tag="VFX11_03150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX4937642.1"
                     /translation="MRRTKIVATLGPATDDPAVLERLLLSGVNVVRLNFSHGAGSDQA
                     RRAEQVRAVAERHDLNIAILADLQGPKIRIGKFTEGKVLLRKDDRFILDANRTELGDE
                     QCVGIDYKALPQDCRRGDILLLNDGSIELEVLAVNGARVETRVILGGPLSNHKGINKK
                     GGGLSAPALTDKDRIDIRSAAEIGVDYVAVSFPKHADDMRLARTLLDECGTHALLVAK
                     IERAEAIETDQILDEIIQASDAIMVARGDLGVEIGDAELIGVQKHLIRRARHLNRLVI
                     VATQMMESMITNQVPTRAEVFDVANAVLDSTDAVMLSAETASGANPVTVVEHMARICE
                     GAEKHPDTRRSRHRNDDIFTRTDESIAMAAMYTANHLQGVKAIVSLTESGNTPLWMSR
                     IRSGLPIFALTRHPQTRRRMALCRGVEALAFDPTSYPYDEVNEAAI"
BASE COUNT          739 a         1204 c         1074 g          685 t
ORIGIN      
        1 taatagagtg aaaaccgtcc tccattcgcc gggttgctct atgccatgca gtcgcgtcaa
       61 gcgtatctcg ataccctcaa aaccctctct gaccgactga tcgcgatcca gcaaccgatc
      121 cgcatcctcg acagcatcaa atggccggcc cagtggcggc aggatttttt caaatccggc
      181 atgaaaacac tgccgccagc cgacagcacc tattaccagt ccattcccct caacttcaat
      241 ttcgacgcca agcaggcgga actgcaggac ctgcgcaagg acatcgtgcg caagctgggg
      301 cgcaaggacg gattgggccg catcctgctg gccaccaccg aacagtacct gaaggtgatc
      361 gacctgctgc gggcgcgggg cacccccgct tttggcggtt tcagcaagga tctgtacggc
      421 tccgcccacg atcacctgtc cggcgacaag cgcaccctgc gggaagtggg cgagcagctg
      481 tgctcgatct tctcgcaccc ggccgcgcag cacatcgcct tttcgcagcc gaagaaatat
      541 gacgccgagg ccgcggtgcg gattctgcgc catcgcctgg ccagctattt tcccgatggc
      601 gaactgcagg tgaagctgtc ggacggtatc gtgtcggacg ccgccgccgg tggcgacagc
      661 atcaagatga atgcccgctc gatgttttcc gagcgggatc tgcaggtact ggaagtgcac
      721 gagggctggg tgcatgtggg caccacgctc aacggccggc gccagccgta tgccagctgg
      781 ctcagtgtcg gttcgccacg cattgccgcc atccaggaag ggctggcact gctgatggaa
      841 accctcacct tcagcagttt tccccaccgc gcccgccgcg tcagcgaccg ggttgccgcc
      901 attcacctgg cggaggaagg cgcggacttc atccaggtat tccgccacct ggcggatcgc
      961 ggcgtgtccg agcccgatgc cttcacgctg acccagcgcg tgttccgcgg cggcctgtgt
     1021 gatggcggtg cggccttcac caaggatctg gcctatgtga agggcttcgt cgagaacgtg
     1081 aatttcatgc gcagcgccat cgccgccggt ctgccggaat tgatccccat gctgttcgtg
     1141 ggcaagatca ccctggacga tatacccgtg ctgtatgaac gctacctgga aggcatgatc
     1201 gagccgccga aatacctgcc cgccatgttc cgcgatctga acggcctgta tgtgtggttc
     1261 ggattttcca acggcatggg aatggtggac ataaagcggg tgcagaaaca ctttctgaag
     1321 aacttcgccc agatacggcc ggtgtgcccg atcgagatgg cgccccgtgc gccggtgtcc
     1381 actgaaccgg agtaagaggt ccactggaaa tatcacattg cactgctagg cttccttaac
     1441 tacaggatgt ccgttgcgat cgctcttgat cattaggtgc acaccatgga aactttgcct
     1501 aactctggcc tgcacaactt cccttccgct catcactgtc atacctgccc actgcaatcc
     1561 cgctgcgtgc cctccagcct gaacctgacc gacctggagc agttcgacca catcattcat
     1621 cgcagccgac cgttgcgccg gggcgagcac ctgtacatgg cggcggaccc gttccgctcc
     1681 atctacatga ttctgcaggg caccatcaag acgtactgcc tgaccgagga tggcgacgaa
     1741 caggtgaccg gctttctcac caccggtgac tgtttcggtt tcgatggtct caacatcatg
     1801 aagcacacca actccgccaa ggcgctggag cgcacccagg tgtgcgagat tcccttcgaa
     1861 cgtctggaac agttgtgccg tcgcataccc agcctgcagc attacgccta ccagctggcc
     1921 agccgccaga tcctgcagga acagaaactg ctgcagttcc tgggcaagcg cagcgccgag
     1981 gcgcgggtgg cctatctgct gctcaacttt atcggccgcg tcaaccacga tcatccactc
     2041 acccgcccca tccagttgcc gatgacacgc tatgacaccg ccaactacct gggtctgagc
     2101 gtggaaaccg tcagccgtat cctgtcgcgc atgcagcagt tgggcgtgat ccggctgcgg
     2161 ggacgactgg tggcgatcca atcccccgct gcgctgaccg agtgcgctca ggtggaaacc
     2221 aacgtcaagc cggtgcgggt catgagcctg taacgcacgc ctgcattccg ttacaacttc
     2281 atccactcca caacggttgc accggttgcg gcaacggcac aaagccacta gccttgctgg
     2341 tgaatttgtc caagcccccg cctgccgccg ctgttacgga gtctgccatg cgccgcacca
     2401 aaatcgttgc caccctcggc cccgccaccg atgatcccgc tgtccttgaa cgcctgctgc
     2461 tgtccggtgt gaatgtggtg cggctgaatt tttcccacgg tgccggcagt gatcaggccc
     2521 gccgcgccga acaggtgcgg gcagtggcgg aacggcacga tctcaacatt gcgatcctgg
     2581 cggacctgca aggcccgaag atccgtatcg gcaagttcac cgagggcaag gtgctgctgc
     2641 gcaaggatga ccgcttcatt ctcgacgcca accgcaccga actgggcgac gaacaatgcg
     2701 tgggcatcga ctacaaggcc ctgccccagg attgccggcg cggcgacatc ctgctgctga
     2761 atgacggcag catcgaactg gaggtgctgg ccgtcaacgg cgcccgggtg gaaacgcggg
     2821 tgattctggg cggccccctg tccaaccaca aaggcatcaa caaaaagggc ggcggtctgt
     2881 cggcaccggc cctcaccgac aaggaccgca tcgatatccg cagtgcagca gaaatcggcg
     2941 tcgattatgt ggcagtgtcc tttcccaaac atgccgacga catgcggctg gcgcgcacac
     3001 tgctggacga atgcggcacc catgcgctgc tggtggccaa gatcgagcgg gccgaagcga
     3061 tcgaaaccga ccagatcctg gatgaaatca ttcaggccag cgatgccatc atggtggcgc
     3121 gcggggatct gggcgtggaa atcggcgatg cggaactgat cggcgtgcag aagcacctga
     3181 tccggcgcgc gcgtcacctg aaccggctgg tgatcgtcgc cacccagatg atggaatcca
     3241 tgatcaccaa ccaggtgccc acccgggcgg aagtgttcga cgtggccaat gccgtgctgg
     3301 acagcaccga cgcggtgatg ctgtcggcgg aaaccgcgtc cggcgccaat ccggtcaccg
     3361 tggtggaaca catggcgcgc atctgcgagg gtgcggaaaa gcatccggac acccgccgct
     3421 cccgtcaccg caacgatgac attttcaccc gtaccgacga atccatcgcc atggccgcca
     3481 tgtacaccgc caatcacctg caaggcgtga aggcgattgt ttccctcacc gaatccggca
     3541 acaccccgtt atggatgtcg cgcatccgtt ccggcctgcc gattttcgcg ctcacccgtc
     3601 atccacagac ccgtcgtcgc atggcgctgt gccggggcgt ggaggcactg gcgttcgatc
     3661 ccaccagtta tccgtacgat gaagtgaatg aagcagcgat ca
//
LOCUS       DASUEG010000163         6038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_625306, whole genome shotgun sequence.
ACCESSION   DASUEG010000163 DASUEG010000000
VERSION     DASUEG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625306"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFX11_03155"
     CDS             <1..662
                     /locus_tag="VFX11_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX4937643.1"
                     /translation="RVSAQNLHRAWVTIPHVTQFDEADITELEAFRVAQKDALKAEGL
                     NLTVLAFLVKACAHVLRQFPKFNSSLDSDGENLVYKKYVHIGFAVDTPNGLVVPVIRD
                     ADKKSVREIVRDMGELSAKAREKKLTPAEMQGACFSISSLGGIGGTAFTPIVNWPEVA
                     ILGVSRSAMKPVWSGKDFQPRLMLPLSLSYDHRVIDGADAARFTTTLSKVLADIRQLL
                     L"
     gene            complement(724..1293)
                     /locus_tag="VFX11_03160"
     CDS             complement(724..1293)
                     /locus_tag="VFX11_03160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4937644.1"
                     /translation="MGNRLSKIYTRTGDDGSTGLGDGSRVAKNHLRVEAYGTVDELNA
                     QIGLLRAELPVGHAEQSFLETVQHDLFDLGGELCIPGYTLLNAECVSEAESEIDKLNA
                     NLPPLKDFILPGGSKAAAVAHVARTVCRRAERRVYTLSQQEAVNEDGLKYLNRLSDYL
                     FVLARVLARENGGQEVLWRQRNTPRHPEA"
     gene            complement(1326..3137)
                     /locus_tag="VFX11_03165"
     CDS             complement(1326..3137)
                     /locus_tag="VFX11_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4937645.1"
                     /translation="MKFASKTLMAMCAVTPTLAVSAESAVTPTLMPVVTVTATRTPKL
                     ADEALASVIVIDREALDSSSGLDLGEVIRFHSGIDIVRSGGAGQQTSIFMRGANSNQT
                     LVMIDGVRVNSGTYGGVNIQNLRPENVDRVEIVKGPRSSLYGSDAIGGVINIITKPSE
                     KTATHVELGGGDNNTAYGTFRQSIRMGKLSASVNAGGIYTDGYPIFLESDEPRGHTNK
                     ESGVDVGYAAENFSVRARYQNNSGTTEYLSFGMPIDQEFTNDLGLLEATYKASEVISA
                     TLRLSNVRDEIDQNQSDDFAHTDRREADLKFDLALPGNQLFTVGGTYTDSDVSALIYG
                     SGYASVVNNHAVYVQDQLQHGAFSLLVAGRHENHEAYGEHTTGELSVGYALNANNRIR
                     ASASTGYRTPDGNQLFGFGGDPALEPEEAENLEIGSTHTFGNWQLGTAIFRNEVKNLI
                     DFVEIGPWIYELQNINNSRLEGVELSVNWQTANWSWKNQAGYTKAIDRDTEEDLSRRP
                     RQSFSSQLEFKQERYSVGGTLLAKSHSDNSAFDDIRLPGHAVLNLHASVKLDQNVKVR
                     LNVENVADKEYGLSANGTDGIYVAQPLTMSASLVLDF"
     regulatory      complement(3244..3439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3508..4416)
                     /locus_tag="VFX11_03170"
     CDS             complement(3508..4416)
                     /locus_tag="VFX11_03170"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4937646.1"
                     /translation="MTPSLSHWHILGAGAIGGLWALRLQAIGCNVTLLEAVLSSPLRQ
                     LCLKADGANHCHAFPVSMVAQSPLLVTTKAGDTIDALRPVLDSLPSGSSLVLLQNGMG
                     VDDLIRQQRPDLRIITGITTDGVFRENRNQLVLAGQGITLLGGETPAEQDIANDIAQQ
                     WSRTGATVSAATDIRIRRWQKLAINCAINPLTARYRCRNGELLDKPEALATMQAICHE
                     VAAVMHAEGMAADGDDLFGKAKEVAFQTGANTSSMLADVQAGRRTEIDFMNGYIVHLA
                     SRHGLTVPANKAITAAIRLLSQTPSA"
     gene            complement(4413..5387)
                     /locus_tag="VFX11_03175"
     CDS             complement(4413..5387)
                     /locus_tag="VFX11_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937647.1"
                     /translation="MPNTDTSILVVDDTKFSSAMVNRVIARAGYQDVRHAHSAADALR
                     LLEERPAHILIADWLMPEMDGLELSQRVRQLDEAGNHFTYVMLLTAKEGVEALAVAFE
                     QGVDDFVNKSHMQDQLLPRLLSAERITTLQNRLLLENQNLIEANAKLKKLSPFDGLTG
                     LGNRAYASRRLADTIKHVSSRGGAACCLLITISNFDAMRKQYPAAIVSQMLLAVARRL
                     RQLVRPLDVVTRIGPNQFAIITHQPDIDHCSAQSYRRIFDGVNLKAIKTAAGFLSIKA
                     AMTIAACDEEALAPTPEALMDLAQSQVNKAIETASIVAARWSSEAQPA"
     gene            complement(5483..>6038)
                     /locus_tag="VFX11_03180"
     CDS             complement(5483..>6038)
                     /locus_tag="VFX11_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4937648.1"
                     /translation="MFSCLYGVGYVIVRYRDSGVLKRYQATPVTALEFLGAQAASRLL
                     ISVATLAVVFAGSHFFLDFPVKGSWLLLLLVTVLGALSMIALSLVCAARIDSLEFMNG
                     LLNFISWPMMLLSGLWFTLDTAPSLVQQLSLLMPLTHLVNAARAIMLDGAGFTAVADE
                     LLVLLLTSIILMAIAARWFRWQKE"
BASE COUNT         1196 a         1693 c         1865 g         1284 t
ORIGIN      
        1 agcgcgtatc agcgcagaat ctgcaccgtg cgtgggtgac catcccgcac gtgacgcagt
       61 tcgatgaggc cgacatcacc gagctggaag cgttccgtgt ggcacagaag gatgcattga
      121 aggcggaagg gctcaacctg accgttttgg ccttcctcgt gaaagcctgt gctcatgtgt
      181 tgaggcagtt ccctaaattc aatagctcgc tcgacagtga tggcgaaaac ctggtgtaca
      241 agaaatatgt gcatattggc tttgccgtgg atacgccgaa tggtctggtg gtgccggtga
      301 ttcgggatgc ggacaagaaa tccgtgcgtg agattgttcg cgatatgggt gaactgtcgg
      361 ccaaggctcg cgaaaagaag ctgacgccgg cggaaatgca gggcgcctgt ttcagcattt
      421 cgtcactggg cggaattggt ggcacggcct tcacgcctat tgtgaattgg ccggaagtgg
      481 ccattctcgg ggtgtcgcgc tcagccatga aaccggtctg gagcggcaag gatttccagc
      541 ctcgcctgat gctgccgcta tcgctttcct atgaccatcg tgtgattgat ggggccgatg
      601 cagcgcgctt cacgaccacc ttgtcgaagg tgttggcgga tatccggcag ttgctgttgt
      661 aagcactgtt ttgtgccaat aaaaagcccg catcgctgcg ggctttttat tggggggtgc
      721 ggtttatgcc tccgggtgtc gcggcgtatt gcgctgccgc cacaggactt cctgtccgcc
      781 gttttctcgg gccagtaccc gggccagcac aaagaggtag tctgaaagcc ggttcaggta
      841 tttcagacca tcctcgttca cggcctcttg ctggctgagc gtgtagacac gccgttcagc
      901 acgccggcaa acggtgcggg ccacatgggc aacggccgcc gctttgctgc cgccaggcag
      961 gatgaaatcc ttcagcggcg gcaggttggc attaagcttg tcgatctcgc tctcggcttc
     1021 cgacacgcat tcagcattca gcagggtgta acccggaatg cacagctcgc cgcccagatc
     1081 gaacaggtcg tgctgaaccg tttccagaaa ggactgctcg gcatgaccga caggcagctc
     1141 ggcgcgcaac agcccgatct gggcattcag ttcgtcgact gtgccgtagg cttccacgcg
     1201 cagatggttc ttggcgacgc gactgccgtc accaaggccg gtactgccat catcacccgt
     1261 gcgggtgtag atttttgaaa gtcggttacc catggctact ccgtgatacc cgttttatgg
     1321 acttatcaga agtccaggac cagactggcg gacatggtca ggggctgggc tacatagatc
     1381 ccgtccgtgc cattggcact cagaccatat tccttgtcgg ccacgttctc gacattcaag
     1441 cgcaccttga cgttctgatc cagcttgacg ctggcgtgaa ggttgagaac ggcatgcccc
     1501 ggcaggcgga tatcgtcaaa ggcagagttg tcgctgtggc tcttggccag caacgtgccg
     1561 cccacactgt aacgctcctg cttgaattcc agctgtgaag agaagctctg gcgtggtcgg
     1621 cgcgacagat cttcttcggt gtcgcgatca attgccttgg tgtagccggc ctggttcttc
     1681 caggaccagt tagcggtctg ccagttgacg ctcagctcca caccttcaag acggctgttg
     1741 ttgatgttct gcagctcata gatccaaggg cctatctcga cgaagtcgat gaggttcttt
     1801 acctcgttgc ggaagatcgc tgtgccaagt tgccagttgc cgaaggtgtg cgtgctgccg
     1861 atttccagat tttcggcttc ttccggctcc agagcaggat ctccgccgaa gccaaacagc
     1921 tggttgccgt ccggtgtgcg gtagcctgtg ctggcgctag ccctgatacg gttgttcgca
     1981 ttcaaagcgt acccgactga caactctccg gtggtgtgct cgccatatgc ctcatgattc
     2041 tcatgacgac ctgccacgag cagcgagaag gcgccatgct gcagttggtc ctgaacgtag
     2101 acggcatgat tgttgacgac agatgcatag ccggagccat agataagggc gctgacatcc
     2161 gagtcagtgt aagtccctcc aacggtgaag agctggttgc ctggcagtgc caagtcgaac
     2221 ttcaggtcgg cttcgcggcg atcggtatgg gcaaagtcgt cagactggtt ttggtcaatt
     2281 tcgtcacgga cattcgacag acgcagggtg gcagaaatga cctcgctggc cttgtaggtg
     2341 gcttcaagca agcccaggtc gttggtgaat tcctgatcaa taggcatgcc gaaagacagg
     2401 tattcagtcg tgccgctatt gttctgatag cgagcacgga ccgagaaatt ttcagccgca
     2461 tagccgacat ccacgccact ttccttgttc gtatggccgc gtggttcatc actttcaagg
     2521 aaaatggggt agccatctgt ataaatgccg cccgcattta cgctggcgct cagcttgccc
     2581 atgcggatgg actggcggaa ggtgccatag gccgtattgt tgtcgccgcc accaagctct
     2641 acatgggtag ccgttttttc cgagggtttg gtaatgatgt tgatgacacc gccaattgca
     2701 tcggaaccat aaagagacga acggggacct ttcacaatct ccacgcggtc aacattttcc
     2761 gggcgcaggt tctggatgtt gacgccgcca taggtgcccg agttgactcg cacgccatca
     2821 atcatgacca acgtctggtt gctgttggcg ccgcgcatga aaatcgatgt ctgctggcca
     2881 gcaccaccag agcgcacaat atcaatcccg gaatgaaagc ggatcacttc tcccagatcc
     2941 agtccgctgc tgctgtcgag tgcttcacga tcaatcacga tgacacttgc cagtgcttca
     3001 tcggccagtt ttggcgtgcg ggtggcggtg acggtgacga caggcatcaa cgtcggagta
     3061 acggcggatt ctgccgacac ggcaagtgta ggggtaacgg cacacatggc catcagggtc
     3121 ttggatgcga atttcatcag tggctccagg ctgtttgggt ccccgcaaac aggaatgtgg
     3181 ccagcatgga ggcaagaaga gtgtgcgcag gcacaagaag tcttccggtc gtgagccctc
     3241 cgcactgacc atggggcttc aggccggtct ccgggctcgt cgcgggatga atccagaccc
     3301 ttcgccttcc cgtctgatcg acagtggcat gatgaagtgg ctgacacgac ttaccgttgc
     3361 gggggcagca tcgggttttc accgatttcc cgtccattcc agtttgcggc aggaacggac
     3421 cagcacgctg agcgtgccgg acacctgaag gcgcggcgat tctagggccc ggttgggtgt
     3481 aaaccaagtg tcatttgcgg ccagccgtca ggcggacggt gtctggctca gcaagcggat
     3541 ggccgccgtg atggccttgt tggcgggcac cgtcagtcca tgtcggctgg ccagatgcac
     3601 aatgtagcca ttcatgaagt cgatttccgt gcgtcgaccg gcctgcacat cggccagcat
     3661 ggaggaggtg ttggctcctg tctgaaacgc aacttctttt gctttgccga acaggtcgtc
     3721 accatcggcg gccatgcctt cggcgtgcat gacggcggcc acctcgtggc agatggcctg
     3781 catggtggcc agcgcctccg gcttgtccag cagttcaccg ttgcggcaac ggtaccgggc
     3841 ggtgaggggg ttgatggcac agttgatggc cagcttctgc cagcggcgta tccggatgtc
     3901 cgtcgcggca ctcactgttg ctccggtacg ggaccactgt tgggcaatat cgttggcgat
     3961 atcctgttcg gcaggggttt caccgcccag cagggtgatg ccttgcccgg ccagtaccag
     4021 ctgattgcgg ttttcccgga agaccccatc ggtggtgatg cccgtgatga tgcgcaggtc
     4081 tggccgctgt tggcggatca ggtcatccac gcccatgccg ttctgcagca gaaccaggct
     4141 gctgccgctt ggcagcgagt ccagcacggg gcgcagggca tcaatcgtat caccggcctt
     4201 ggtggtcacc agcaaaggcg attgcgcgac cattgagacc gggaaggcat ggcagtgatt
     4261 ggcgccatcg gctttcaggc agagctggcg caagggggag gagaggacgg cttcaagcag
     4321 ggtaacgttg cagccgatgg cctgcaggcg caatgcccac aggccgccga tcgcaccggc
     4381 accgagtatg tgccagtgcg agagggaggg agtcacgctg gctgggcctc actgctccag
     4441 cgtgctgcga caatcgacgc cgtctcgatg gccttgttga cctgggactg tgccaggtcc
     4501 atcagggctt ccggtgttgg cgccaatgct tcttcatcgc aggcggcaat cgtcatggcg
     4561 gccttgatgg agagaaagcc ggcagccgtc ttgatggctt tcaggttcac gccatcgaaa
     4621 atgcggcgat aactctgcgc actgcaatga tcaatgtcgg gctggtgcgt gatgatggca
     4681 aactggttgg gaccgattcg cgtcaccacg tcaagggggc gtaccagttg acgcaggcgg
     4741 cgtgccaccg ccagcagcat ctggctgaca atggcggccg ggtactgttt gcgcatggcg
     4801 tcgaagttgc tgatggtgat cagcaggcag caggcagcgc cgccgcgcga ggagacatgc
     4861 ttgatggtgt cggccaggcg gcgtgacgca taagcccgat tgcccagccc ggtcagcccg
     4921 tcaaacggtg acagcttctt gagcttggcg ttggcttcaa tcaggttctg gttttccagc
     4981 agcagacggt tctgcagagt ggtgatgcgc tcagccgaca acagtcgtgg cagcagttga
     5041 tcctgcatgt gggacttgtt gacgaagtcg tcgacaccct gctcgaacgc cacggccaag
     5101 gcctcaacac cctcttttgc cgtcagcagc atgacatagg tgaagtggtt gccggcctcg
     5161 tccagctgac gcacacgctg gctcagttcc agcccgtcca tttccggcat cagccagtca
     5221 gcaatcagga tatgggccgg tcgctcctcc agcaatcgga gggcatcggc tgccgagtga
     5281 gcatgacgca cgtcctgata gccggcgcga gcgatgacac ggttgaccat cgcactggaa
     5341 aacttggtgt cgtccaccac caggatcgag gtatcggtat tcggcatatt attattctca
     5401 ggcatccgat gcccgttttg tgaagtcgtt cctttccttt aactgcccct tgtgtcgccg
     5461 tcaagcagcg caggacaaaa ggtcattcct tctgccagcg gaaccagcgg gcggcgatgg
     5521 ccatcaggat aatcgaagtc agcagcagga ccagaagctc gtccgcaacg gcagtaaaac
     5581 cggcgccatc cagcatgatg gcgcgtgctg cgttaaccag atgcgtgagt ggcatcagca
     5641 gggaaagctg ctggaccagc gatggtgccg tatccagtgt gaaccagagg cccgagagca
     5701 gcatcatggg ccaactgatg aagttgagca gcccgttcat gaattccagg ctgtcgatgc
     5761 gggccgcaca gaccaggctc agggcgatca tgctgagcgc gcccagtacc gtgaccagaa
     5821 gaagcagcag ccaggagccc ttgaccggaa agtcgaggaa gaagtggctg ccggcaaaga
     5881 caacggcaag ggtggcaacg ctgatcagca ggcgcgaggc agcctgcgcg cccagaaact
     5941 ccagtgcggt cacgggcgtt gcctgatagc gcttcagtac gccgctgtcc cgatagcgca
     6001 cgatgacata acccactccg tacaggcagg aaaacatc
//
LOCUS       DASUEG010000164         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_581587, whole genome shotgun sequence.
ACCESSION   DASUEG010000164 DASUEG010000000
VERSION     DASUEG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581587"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /gene="rne"
                     /locus_tag="VFX11_03185"
     CDS             complement(<1..1991)
                     /gene="rne"
                     /locus_tag="VFX11_03185"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HEX4937649.1"
                     /translation="MKRMLINASHAEEKRVAMVDGQRLYDFDLEHTAREQKKASIYKG
                     KVTRIEPSLEAAFVDYGADRHGFLPLKEIAREYFNKSPKEAGGRITIRDAIKEGQEII
                     VQVEKEERGNKGAALSTFISLAGRYLVLMPNNPRAGGISRRIEGEERQELKDALNAIN
                     IPQDMGAIVRTAGLGRSAEELQWDLDYLMTLWTSIKTASQDRAAPFLIYQESNVIIRA
                     VRDYLRQDINEVLIDAEPAYNEAINFVQQVMPHYQNKIKLYKDSVPLFNRYQIESQIE
                     TAYQREVKLPSGGSIVIDPTEALVSIDINSSRATKGGDIEETALNTNLEAADEIARQL
                     RLRDIGGLIVIDFIDMTPAKHQRQVEDRLRDALSMDRARIQIGRISKFGLLELSRQRL
                     RPSLEETTGLVCPRCNGTGVIRDVRSLALSIMRVIEEEVLKERTAQINAQVPISVATF
                     LLNEKRAALASLEGRSGVRVVILPNEHLETPHYEVTRLRDDQIEEGQEDVPTYKMVER
                     IQPPANEDVLKAVDPEPRRQEAAVKMIQHQTQAPAPKPAAPAPLAPVAAEPSKQGFFA
                     WLSNLFGGGEKAPEPVVEETPKKPEGSNRRRNDRDRRDGNRQGGRNRNEGNREGREAR
                     ENREPRPEREPREPREPKEAREPRESSRGEGRGQGGENRR"
BASE COUNT          448 a          764 c          845 g          567 t
ORIGIN      
        1 cggcgatttt caccgccctg gccacggcct tcgccacggc tgctctcgcg cggctcgcgg
       61 gcttccttgg gttcacgggg ctcacgaggt tcgcgttcag gacgcggttc gcgattctca
      121 cgtgcttcgc ggccttcacg attgccttcg ttacggttgc ggccaccctg acgattacca
      181 tcgcggcggt cacgatcatt gcggcggcga ttgctgcctt ccggcttctt cggtgtctct
      241 tcgacaacag gctctggagc cttctcgcca ccgccaaaca ggttggacag ccaggcaaag
      301 aagccctgct tgcttggttc ggctgccaca ggtgccagcg gggcaggggc agcaggcttg
      361 ggagcagggg cctgggtctg gtgctgaatc atcttcaccg ccgcttcctg acggcgcggc
      421 tccggatcca cggctttcag cacgtcttca ttggccggcg gctggatgcg ttccaccatc
      481 ttgtaggtcg gcacgtcttc ctggccttcc tcgatctggt catcacgcag gcgagtcact
      541 tcgtaatgcg gggtttccag atgttcgttc ggcaggatca ccacgcgcac accgctacgt
      601 ccctcaaggg aggccagtgc agcacgcttt tcgttgagca ggaaggtggc gacggagatc
      661 ggcacctgtg cgttgatctg cgcggtacgt tccttcagca cttcctcttc aatgacgcgc
      721 atgatggaca gggccagcga gcgcacatca cggatgacgc cagtgccatt gcaacgcggg
      781 caaaccagtc cggtggtttc ttccagcgac ggacgcagac gctggcgcga cagttccagc
      841 agaccgaact tggagatgcg gccgatctgg atacgggcac ggtccatgga caaggcatcg
      901 cgcagacggt cttccacctg acgctggtgc ttggctggcg tcatgtcgat gaagtcgatg
      961 acgatcaggc cgccgatatc gcgcaggcgc agctggcggg cgatttcgtc tgccgcctcc
     1021 agattggtgt tgagcgcggt ttcctcaatg tcgccgccct tggtggcgcg cgaggagttg
     1081 atgtcgatgg acaccagtgc ttcggtcggg tcgatcacga tggaaccgcc cgacggcagt
     1141 ttcacttcac gctggtaggc cgtctcgatc tggctttcga tttggtaacg attgaacagc
     1201 gggaccgagt ccttgtagag cttgatcttg ttctggtagt gcggcatcac ctgctgtacg
     1261 aagttgatcg cttcgttata ggcaggttcc gcgtcgatca ggacttcatt gatgtcctga
     1321 cgcaggtaat cgcgcacggc acggatgatg acattacttt cctgatagat caggaagggc
     1381 gcagcacggt cctggctggc cgtcttgatc gacgtccaca gggtcatcag gtaatccaga
     1441 tcccactgca gttcttcggc gctacggccc aagccggcgg tacggacgat ggcacccatg
     1501 tcctgcggga tgttgatggc gttcagggca tccttcagct cctgacgttc ttcaccctcg
     1561 atgcggcggg agatgccacc ggcgcgcggg ttgttcggca tcagcaccag ataacggccg
     1621 gccagcgaga tgaaggtgga gagggcggca cccttgttgc cacgttcttc cttctcgacc
     1681 tggacgatga tttcctggcc ttccttgatg gcatcgcgaa tggtgatgcg gccgccggct
     1741 tctttcggcg atttgttgaa gtattcgcgg gcgatttcct tcagcggcag aaagccgtga
     1801 cggtctgcgc cgtaatccac aaaagcggct tcgaggctgg gctcgatgcg ggtgaccttg
     1861 cccttgtaga tgctggcttt tttctgttcg cgggcggtgt gttcgagatc gaaatcgtaa
     1921 agacgttgtc cgtccaccat cgcaacgcgt ttttcttcgg cgtgcgaggc attgataagc
     1981 atgcgtttca tgttctaccc aacgggttcc ggcactcaag gcaggaacgc cgccggcagg
     2041 ggcgacggat gtgacgtgac tgtggtctcc ggtcgagaca acacagtgcg gcttccgctt
     2101 gccgggcaaa gccggcgtga tgcagcagaa tccgcatcgt tcacctgatg ccggaaatcg
     2161 cgggcttgtg gcccggaatc agactgatgg cctgaccggc ggggaaacac cgtaatgtct
     2221 cccggaattc attcgtaacc cgctatcgac agcgtcccat ttgccaggtg caaatggttt
     2281 caaccggcgg acgtgccgcc acatgtgaga gtgcctgtac tgaccgggcc ggcaatgccg
     2341 gaaggcgggc ggggcaggca gcctcggttg ccacaacggg gaagtgcatg gattgcggcc
     2401 tggtcaaccc cgtacaaagg gggtgagggc cggcgaggtg cggtccctgc cgtcatggct
     2461 gtcactggca gtcgtggaac ggggtcgggc agctgcacgg tgcctcacgc acatcgggcc
     2521 gggctaaggc cgggcgggat ggcgggcaag aaactgcagc cgttcgcgac actggttcag
     2581 ggcttcctgt acaatggaaa ccctactggc tggcgggcgg ttac
//
LOCUS       DASUEG010000165         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_462857, whole genome shotgun sequence.
ACCESSION   DASUEG010000165 DASUEG010000000
VERSION     DASUEG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462857"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFX11_03190"
     CDS             <1..469
                     /locus_tag="VFX11_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX4937650.1"
                     /translation="RSPQVMLGYWNLPEETAKSITDDGWFKSGDAGYIDADGYVYIHD
                     RVKDMIVSGGENVYPAEVESVLFGLEGIADVAVIGVPDDRWGEAVKACVVLKPGASLS
                     AQDIMAFARERIAGYKVPKSVDFIAALPRNPSGKILKRELRAPYWHGRDRQVN"
     gene            765..>2009
                     /locus_tag="VFX11_03195"
     CDS             765..>2009
                     /locus_tag="VFX11_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937651.1"
                     /translation="MKPLSGFALALLATIPLLPGCGGGGSSSSGSNSDRAATTAEAGT
                     TSTPSTAFDSHAFFDARIAPRLNFCATCHVPGGLADTDAGDGLLLSGNSASRYDDFYA
                     AWRELGEGTTANPILVENAVLSEPHSGGKSWPAGSEPYRDVAQLFGCWDDPVSCAEQT
                     EEEPPEVVDDADDLPLLESGPIKTPLVAGCDGKPDDTPLPLDPRSLVVEGVNKPGVAV
                     QFNAYWVDVHNPLPPYRANPLQPAATCGEWKARVAAGGELVKFGSRFMGGLRLFTPRN
                     YRNLWKSWGLKERPANFDEEVLKRYGLFKAEFRNPYPLDGEDPNQTDGGSGQLPLGLA
                     MIKDRDGRYNGEMTVNCSFCHDSRLGTDEEGQQFHWGRGSNFMDIGVFVNDFTKRLAG
                     IPAIAPYPFGAHAGGSNALGVID"
BASE COUNT          401 a          656 c          605 g          347 t
ORIGIN      
        1 gcgctcgccg caggtcatgc tcgggtactg gaacctgccg gaggagacgg ccaagagcat
       61 caccgacgac ggttggttca agagtggcga cgccggctac atcgacgccg acggctacgt
      121 ctacatccac gaccgcgtga aggacatgat cgtgtccggc ggcgagaacg tgtatccggc
      181 ggaagtggaa agcgtgctgt tcggcctgga gggcattgcc gacgtggccg tgatcggcgt
      241 gcccgatgac agatggggcg aagcggtgaa agcttgcgtg gtactgaaac ccggtgccag
      301 cctgtccgcg caggacatca tggcctttgc gcgggagcgg atcgccggtt acaaggtgcc
      361 gaaatcggtg gacttcattg ccgcactgcc ccgcaatccc tccggcaaaa tcctgaaacg
      421 ggaattgcgg gcgccgtact ggcatggccg cgatcgtcag gtgaactaga accagcaccg
      481 ctgccggcag ccggatatgc tggcagcaaa agtgtgctat ttcatcccgc gactgcctga
      541 ttttccagca cttgcgaagg acgacagcca tcccctttcg ctttttgacg ccttgacacc
      601 ccggtgccac catttcgtga ccgattggtc acttcgctta cgacaagcgg atcacaccat
      661 cgaaaaccgg cgctgacacc ttcgcaaagg tctgtcccgc agtctccttt ctctgtcgcc
      721 gttcgccatc caaaacaaga atgaagcaag caaaggagaa caccatgaag cccttgagcg
      781 gtttcgcatt ggcattgttg gccaccatcc ctctgctgcc tggatgtggc ggcggaggtt
      841 catcctcctc cggcagtaac agcgaccggg cggcaacgac ggcagaagcc ggcacgactt
      901 ccaccccatc cactgccttc gacagccacg cctttttcga cgcccgcatc gccccgcgcc
      961 tgaatttctg tgccacctgc catgtaccgg gcggtctggc ggataccgac gcaggcgacg
     1021 gcctgctgct gtccggcaac agcgccagcc gctacgacga cttttacgcc gcctggcgtg
     1081 aactgggcga aggcaccacg gccaacccca tcctggtgga aaacgcggtc ctgtccgaac
     1141 cccacagcgg cggaaaaagc tggccggccg gcagcgaacc ctaccgggac gtggcacaac
     1201 tgttcggctg ctgggacgac cctgtcagct gcgcagaaca gacggaagaa gaaccaccgg
     1261 aagtggtgga cgatgccgat gacctgcccc tgctggaatc cggcccgatc aaaacgccgc
     1321 tggtcgccgg ctgcgacggc aagcccgatg acaccccgct gccgctggac ccgcgcagcc
     1381 tggtggtgga aggtgtgaac aagcccggcg tggcagtgca gttcaatgcc tactgggtcg
     1441 acgtgcacaa tccgttgcca ccgtaccgcg ccaatccgct gcaacccgcc gccacctgcg
     1501 gcgagtggaa agcccgcgtc gctgccggtg gggaactggt caaattcggc tcgcgcttca
     1561 tgggcggcct gcgcctgttc acgccacgca attatcgcaa tctctggaaa tcctggggcc
     1621 tcaaggaacg gcccgccaat ttcgacgagg aagtgcttaa gcgctacggc ctgttcaagg
     1681 cggaattccg caatccgtat ccgctggacg gcgaagatcc caaccagacc gacggcggca
     1741 gcggccagtt gcccctgggc ctggcgatga tcaaggatcg ggatggccgc tataacggcg
     1801 agatgaccgt gaactgttcc ttctgccacg attcacgtct gggcaccgac gaggaaggcc
     1861 agcagttcca ctggggccgc ggcagcaact tcatggacat cggcgtgttc gtcaacgatt
     1921 tcaccaaacg ccttgccgga attcccgcca tcgcacccta cccgttcggt gcccatgccg
     1981 gcggctccaa cgcactgggc gtgatcgac
//
LOCUS       DASUEG010000166         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_994931, whole genome shotgun sequence.
ACCESSION   DASUEG010000166 DASUEG010000000
VERSION     DASUEG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994931"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFX11_03200"
     CDS             <1..938
                     /locus_tag="VFX11_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4937652.1"
                     /translation="ILKEEIQHKNPTGMQAFVMNTRRDLFMDARVREALDYAFDFEWT
                     NKQLFYGAYKRTNSFFSNSDLAAQGIPTDKELALLEPYRDDLPPQLFTQEYQSPTTDG
                     SGKNRSNIRKAVELLKAAGWEYRGTDLVNTATGQPFKFEILLYSKDFERIALPFIKNL
                     KKLGIEASARIVDTTNYVRIVRDFDFDMIIGGFAQSNSPGNEQRDYWFSHFADHKGSQ
                     NSIGVKDPIVDALIDRIVSAESREDLVAACRALDRVLLWNHYVIPQWHINTHRIAYWN
                     KFARPAIAPIYGDVGFMTWWLDPAKAATLTPVEKP"
     gene            940..2016
                     /locus_tag="VFX11_03205"
     CDS             940..2016
                     /locus_tag="VFX11_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEX4937653.1"
                     /translation="MLAYIIRRLLLIIPTLLGILTLNFFIIQAAPGGPVEQMIAQLEG
                     LEGGATARMGGGTQAEVRSSNSQYRGAQGLDPELIKRIEKMYGFDKPIGERFLIMLKS
                     YAMLDFGNSFFRDKKVTELILEKMPVSISLGLWSTLLIYLISIPLGIRKAVSNGSRFD
                     VWTSSVVIIGNAIPSFLFAILLIVLFAGGSYYQLFPLRGLTSPDFDEMSALGKILDYF
                     WHLVLPIGSLVIGGFATLTLLTKNSFLDEISKQYVVTARAKGLTDSKVLYGHVFRNAM
                     LIIIAGFPGALVGIFFTGAFLTEIIFSLDGLGLLSFESVLSRDYPVIFGTLFIFTLIS
                     LLVNLISDITYTLVDPRIDFESRN"
     gene            2020..>2355
                     /locus_tag="VFX11_03210"
     CDS             2020..>2355
                     /locus_tag="VFX11_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4937654.1"
                     /translation="MSRKPFKLTPMNQRRLQRFKANRRGWYSLWIFTALFIVSLGAEL
                     IANDKPLLIKFDDQYYFPVLFTYAEQEFGGELPTEADYRDEYVLELIQEKGWIMYPPV
                     RFSYNTINYD"
BASE COUNT          514 a          693 c          608 g          540 t
ORIGIN      
        1 aaatactgaa agaggaaatc cagcacaaga atcccacagg catgcaggct tttgtgatga
       61 acacccggcg cgatctgttc atggatgccc gcgtgcggga agccctggac tatgcgttcg
      121 acttcgagtg gaccaacaag cagttgttct acggagccta caaacgtacc aacagcttct
      181 tctccaattc ggatctggcg gcgcagggaa ttcccaccga caaggaactc gccctgctgg
      241 agccttaccg cgacgatctg ccgccgcaac tgttcaccca ggaataccag tcccccacca
      301 cggatggcag cggcaagaac cgcagcaaca tccgcaaggc cgttgaattg ctcaaagccg
      361 ccgggtggga atacaggggc acggatctgg tgaacaccgc caccggccag ccgttcaagt
      421 ttgagattct cctttacagc aaggatttcg aacgcattgc cctgcccttc atcaagaacc
      481 tgaaaaaact cggcatcgag gccagcgccc gcattgtcga tactaccaat tatgtgcgca
      541 ttgtccgcga cttcgatttc gacatgatta tcggtggttt cgcccagagc aactcccccg
      601 ggaacgaaca gcgcgattac tggttctccc atttcgccga ccacaagggc agccagaatt
      661 ccatcggcgt gaaggacccg attgtggatg cactgatcga caggattgtg tccgcggaat
      721 cccgcgagga tctcgttgct gcctgccgcg ccctggaccg cgtgctgctg tggaatcact
      781 acgtcatccc gcagtggcat atcaacaccc accgtattgc gtactggaac aagtttgccc
      841 ggccggccat tgcgcccatt tacggcgacg tgggattcat gacctggtgg cttgatcctg
      901 ccaaggccgc cacactgacc ccggtggaaa aaccctgaca tgctggcgta tatcattcgt
      961 cgcctgctgt tgatcattcc gaccctgctg ggcattctca ccctgaattt tttcatcatt
     1021 caggctgcgc ccggcggccc ggtggaacaa atgatcgccc aactggaagg gctggaagga
     1081 ggcgccactg cgcgcatggg cggcggcacc caggcggaag tccgcagcag caattcgcag
     1141 tatcgcggcg cacaggggct ggatcccgaa ctgatcaagc gtatcgaaaa gatgtacggc
     1201 ttcgacaaac ccatcggcga acgcttcctg atcatgctga aaagctatgc catgctggat
     1261 tttggcaaca gtttctttcg cgacaagaaa gtcaccgagc tgatcctgga aaaaatgccc
     1321 gtatcgattt cactgggcct gtggtccaca ttgctgattt atcttatctc gattccgctg
     1381 ggcatcagga aggccgtgag caatggttca cgtttcgatg tctggaccag ttctgtggtc
     1441 atcattggaa acgccatccc ctcgttcctg tttgccatcc tgctgatcgt actgtttgcc
     1501 ggcggcagct attaccagct gtttccgctg cgaggcctca catcaccaga tttcgacgag
     1561 atgtcagcgt taggcaagat actcgactat ttctggcacc tggtattgcc catcggttca
     1621 ctggtgatcg gtggtttcgc caccctgacc ctgctcacca aaaattcgtt tctggacgaa
     1681 atcagcaagc aatatgtcgt gacggcgcgg gccaagggac taaccgattc caaggtgctc
     1741 tacggccacg tcttccgcaa cgccatgctg atcatcattg cgggatttcc cggcgccctg
     1801 gtaggtattt tcttcaccgg cgccttcctg acggaaatca ttttttccct ggatggcctg
     1861 ggtctgctga gttttgaatc cgtactgtcg cgggactatc cggtgatttt tggcaccctg
     1921 ttcatcttta cgctgatctc gctgctggtg aatctgatca gcgacattac ctacactctc
     1981 gtggatcccc gcattgattt cgagtcgaga aattaacgga tgagtcgcaa accgttcaaa
     2041 ctcactccca tgaaccagcg ccgcctgcag cgcttcaagg ccaaccggcg cggctggtat
     2101 tccctgtgga tctttactgc cctgtttatc gtcagcctgg gcgcggaact gatcgccaac
     2161 gacaaaccgc tgctgatcaa atttgacgat cagtattatt ttcccgtgct gttcacctac
     2221 gctgaacagg agtttggcgg cgaactgccg acagaggccg attaccgcga cgaatacgta
     2281 cttgagctga tccaggaaaa gggctggatc atgtacccgc ccgtgcgctt cagttacaac
     2341 accatcaatt acgat
//
LOCUS       DASUEG010000167         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1685715, whole genome shotgun sequence.
ACCESSION   DASUEG010000167 DASUEG010000000
VERSION     DASUEG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685715"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFX11_03215"
     CDS             complement(<1..336)
                     /locus_tag="VFX11_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4937655.1"
                     /translation="MTTPTPEWKKTACILCSLNCGLEVQTANGRITKIRGDDDHPASH
                     GYVCEKSQRMDYYQNGADRLDSPMRRRPDGSYEAIDWDTAIREVAQKFLAIKQKHGGE
                     SILYYGGGAQ"
     gene            complement(402..740)
                     /gene="fdxA"
                     /locus_tag="VFX11_03220"
     CDS             complement(402..740)
                     /gene="fdxA"
                     /locus_tag="VFX11_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX4937656.1"
                     /translation="MTFVVMEECIRCKHTDCVEVCPVDCFYEGPNFLVINPEECIDCA
                     LCEPECPVDAIRAEDEVPDDQQAFIVLNAELSRHPNWTQITRKKPAPDDHEQWKGVKG
                     KLAELDRNGA"
     gene            complement(737..3025)
                     /locus_tag="VFX11_03225"
     CDS             complement(737..3025)
                     /locus_tag="VFX11_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4937657.1"
                     /translation="MTMEKTACILCSRNCGLTVDIEDGKFVRIRGDEDHPVSKGYICQ
                     KAARLEHYQNHGDRLTHPLKRQPDGSFVQVSWDEALDDIAQRLLRIRQRHGGDAFAFV
                     GGGGQGNHLGGAYSRQILAAMKSRYAYNALGQEKTGDFWLNGRLFGSQTCHTTEDVEH
                     ADYVLFIGTNPFQAHGIPNARDTLRDLKKDPNRTMVVIDPRRSETAKQADIHLQLKPG
                     TDAYLMSAMLAIIVREGLHDREFIARHCTGFDDVEKALKAIPVEEYARRADVPLADIE
                     RVARGYATARTACVRIDLGIQQTLHTTLNGYLEKLLYLITGNFGIKGGNNLHSFLLPI
                     LGNTDERKKRKGKSLQLTAHHKMHPIAGIYPPNLLPDEILLAGEKRIRSVFVDSANPL
                     LTYADTGAYEKAFQSLELLVVVDVAMSETARLAHYVLPAASQFEKWEATGFNLEFPEN
                     AFHLRHPLFPSLGDTLPEPEIYTRLLEKMGEIPARFPRLERIARFEPEASAHMAYMAA
                     LGVTLASNKAWIPHAASVVYRTLGKALPDGAAAAAPLLPLSIQYTNTHYAAVKRAGHE
                     GNRLTLGTSLFRAILEKRAGTVMSLHDFREMWSFIRHKDGRIHLAIPEMLQELRALAS
                     ETPPGANFPFILMAGERRSYNANQIYRDPAWRKVDPHGSMRMHPDDAAELNISDGETV
                     SCASARDSINVVVEIDDSVRRGMVTLPHGYGQRYKGSAPIGPELNRLTSSTHCDPLSR
                     TPYHKYVPVHIQKIATEEEMPA"
     gene            complement(3085..3546)
                     /locus_tag="VFX11_03230"
     CDS             complement(3085..3546)
                     /locus_tag="VFX11_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEX4937658.1"
                     /translation="MNALASFNRLKNLPGGTRLFSRGVCIAAPYFSSIKPHVEVLEPD
                     LARVSIKKRRAVTNHLGTVHAIAMCNMAELAGGLMTDVSIPPTHRWIPKGMTVEYLKK
                     AGTDLVATARPTTTPDFSQAGEYPVQVEVTDTLGTPVFRALITMWVSPKKA"
     gene            3660..4229
                     /locus_tag="VFX11_03235"
     CDS             3660..4229
                     /locus_tag="VFX11_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937659.1"
                     /translation="MLAALACFTEHGVEATSIDMIREASGASVGSLYHHFGNKEGLAA
                     EVYLEGLRDFRQFLAEALRPVTGLEAATRAIVHANVDWICANPDWARFIFGYRMVLAK
                     ANRETDLQAEAQGAQSAIVAQLERLLAPGQNMPWPPEVYHSLVIGPTHDYARNWLEGR
                     RQRPLAELREVFADAAWNSLGFSASAKKG"
     gene            complement(4258..5160)
                     /locus_tag="VFX11_03240"
     CDS             complement(4258..5160)
                     /locus_tag="VFX11_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937660.1"
                     /translation="MRQPDLFVQSGEVRLAVYTWGQKPDAKHPRETVVLAHGFPDRAL
                     FWEKVATTLQHDFHVVAFDMRGCADSTHISGTKYYHFAPLLKDLYAVIDAVSPGRKVH
                     LVGHDWGGIYGWDAICEPEGIKRIASFVTMSPSLDHVGFYLRRRFLKPTPRNLLQAFG
                     QLLRNSLMTFFTLPLLPELLFRSGLGNVMFRKLVAHYEPRITFQKNDGMEGDAIRYLG
                     IYRANLLQRALLPKKHTSPVPVHVLMAVNDPFLPPAVFEVSPETASRYSHSPIDAAHW
                     APLARPLEMAVTVRDFVQKHGRHA"
     gene            complement(5186..>6223)
                     /locus_tag="VFX11_03245"
     CDS             complement(5186..>6223)
                     /locus_tag="VFX11_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937661.1"
                     /translation="ALTAPHHIYAHLVAWIALWIFGALALVSIAMALRALKVRRIFEH
                     QAWMALSFGCLMVAPLLRLDWVLLAWLFPAIDQETLSLVTVGIMLPEVLIIAYGLILV
                     NRQYARPMTKRPAAAIAGHATELFRRALPALHVMALLLLGVNVAFYIAGQGMSSLDAG
                     HTLVPAALVVHEQSVLASHPFIAVLFALSLSLAFPAVVQRLNGLLQQAPSHAFVNHAA
                     PWLALTAGLTSCVIGANIGLAPGKALLSGGTMYMVNGLVLTGFSLLLLAGQRVGQHAL
                     MKESLVFLLCLLPFPALFFLTLQVMSLLPVPADYITAGQGFVVPVGFSGGLLFMAMIY
                     VVYGQATREHN"
BASE COUNT         1253 a         1758 c         1924 g         1288 t
ORIGIN      
        1 ctgcgcgccg ccaccgtaat acaggatgct ttcaccgcca tgtttctgct tgatggccag
       61 gaatttctgc gctacttcac ggatggccgt gtcccagtcg atggcctcat agctgccgtc
      121 cgggcgacga cgcatggggg agtccagacg atcggcaccg ttctggtagt agtccatgcg
      181 ctgtgatttt tcgcagacat aaccgtgcga ggcaggatga tcatcgtcgc cacgaatctt
      241 ggtgatgcgt ccgtttgcgg tctgtacttc gaggccgcag ttgaggctgc agaggatgca
      301 ggcggttttt ttccattccg gagtcggtgt tgtcatggtt ctttactcca aaagagttcg
      361 tgacgattcc ctgcagggca catggtgcca gcagcggcgg gtcaggcgcc gttgcggtcc
      421 agctcggcca gtttgccctt aacccctttc cattgctcgt gatcgtccgg tgccggtttc
      481 ttgcgggtga tctgcgtcca gttggggtgt cgggacagct cggcattcag gacaatgaag
      541 gcctgctggt cgtccggaac ttcatcctct gcacggatgg catcaaccgg gcactccggt
      601 tcacacaatg cacagtctat gcactcttcc ggattgatca ccagaaaatt ggggccctca
      661 tagaagcaat cgaccgggca gacttccacg cagtcggtgt gtttgcagcg aatgcattct
      721 tccatcacga caaaggtcat gccggcatct cctcttcggt ggcaattttc tggatatgga
      781 ccgggacata cttgtggtac ggcgtgcgcg acagggggtc gcaatgcgta ctggaggtaa
      841 gacggttcag ttcgggcccg atcggggcgc tgcctttgta gcgctgacca tagccatgcg
      901 gcagcgtgac catgccacga cgaacggagt catcaatttc cacaacgaca ttgatgctgt
      961 cgcgtgcgga agcacaggaa accgtttcgc catcgctgat gttcagctct gcggcatcgt
     1021 cgggatgcat gcgcatggag ccatgcgggt ccaccttgcg ccaggcggga tcgcggtaga
     1081 tctgattggc attgtagctg cggcgttcgc ctgccataag gatgaacggg aagtttgcgc
     1141 ccggcggtgt ttcgctggcg agggctcgca gttcttgcag catttccggg atggcaaggt
     1201 gaatgcggcc atccttatgg cggatgaaac tccacatttc ccggaagtca tgaagactca
     1261 tcacggtacc ggcccgcttt tcaagaatgg cccggaacag tgatgtgccc agtgtcaggc
     1321 ggttgccctc atggccagca cgcttcacgg ccgcatagtg cgtgttggtg tactggatgg
     1381 acaagggcag cagtggggct gcggccgcgg cgccatcagg cagtgccttg cccaatgtgc
     1441 gatatacgac ggaggcggca tgcggtatcc aggctttgtt gctcgccagg gtgacaccga
     1501 gcgctgccat gtaggccata tgtgccgacg cttccggttc aaatcgtgca atgcgctcca
     1561 ggcgaggaaa gcgtgcgggg atttcaccca tcttttccag caggcgcgta tagatttctg
     1621 gttcgggcag cgtatcaccc agggacggga acagcggatg gcgcaaatgg aaggcgtttt
     1681 ccgggaactc cagattgaag ccggtggcct cccatttctc gaactgtgag gcggccggca
     1741 acacatagtg tgccaggcgc gcggtttcgg acatggcgac atccaccacc accagcagtt
     1801 ccagggactg gaaggccttt tcgtaggctc ccgtatcggc ataggtgagg agtggattgg
     1861 cactgtcgac aaataccgag cgtatccgct tctcgcctgc cagcaggatc tcgtcgggca
     1921 ggagattggg gggatagatg cctgcaatcg gatgcatctt gtggtgtgcc gtcagctgga
     1981 gtgacttgcc tttccgcttc ttgcgttcgt cggtgttgcc cagaatgggt agcagaaagc
     2041 tgtgcaggtt gttgccgccc ttgatcccga agttgccggt gatgagatac agcagctttt
     2101 ccagatagcc gttcaacgtg gtgtgaagcg tttgctggat gccgaggtcg atgcgcacac
     2161 aagcagtacg ggcggtggca taaccgcgtg ccacgcgttc gatatcggcc agggggacgt
     2221 cggcccgccg ggcatattct tccaccggaa tggctttcag tgctttttcc acgtcatcga
     2281 agccggtgca gtgcctggca atgaactcac ggtcatgcag tccttcgcgc acgatgatag
     2341 ccagcatggc gctcatcaga taggcatcgg tgccgggctt caactgcagg tgaatatcgg
     2401 cctgcttggc cgtttctgac cgacgtggat cgattaccac catcgtccgg ttggggtctt
     2461 tcttcagatc gcgcagggtg tcacgggcat tcggaatgcc gtgagcctga aacggattcg
     2521 taccgataaa gagcacgtaa tcggcatgtt ccacatcttc cgtggtatgg caggtctggc
     2581 tgccaaagag gcggccattc agccagaagt ccccggtttt ttcctggccc agcgcgttgt
     2641 aggcatagcg gcttttcatt gcggcgagaa tctgccggct gtaagcgccc cccagatggt
     2701 tgccttggcc gccaccaccc acaaaggcga acgcatcacc accgtgccgc tggcgaatcc
     2761 tgaggaggcg ttgggcgatg tcatccagcg cttcgtccca gctcacttgc acaaagctgc
     2821 cgtcaggctg acgcttgagc ggatgagtca ggcgatcgcc atgattctgg taatgctcaa
     2881 ggcgtgcggc cttctggcag atataccctt tggagacggg atgatcctcg tcgccgcgga
     2941 tgcgcacgaa cttgccgtct tcgatatcga ccgtcagtcc gcaattgcgc gaacagagga
     3001 tgcaggcggt tttttccatt gtcatggcgg actcctggtg ccgttattca tgaatcccga
     3061 actttcccgc tccgggcgaa ggggtcatgc tttcttgggg ctgacccaca tggtgataag
     3121 ggcacgaaac acaggcgtac cgagggtgtc cgtcacttca acctgcaccg gatactcgcc
     3181 tgcctgtgaa aagtcaggcg tggtggttgg tcgggcggtg gcaacaagat cggtcccggc
     3241 cttcttcagg tattccacgg tcatgccctt cggaatccag cggtgggtcg gagggatgga
     3301 gacatccgtc atcaggccgc cggccagttc ggccatgttg cacatggcaa tggcatgcac
     3361 ggtgccgaga tggttggtga cggcgcggcg ctttttgatg ctgacccggg caaggtccgg
     3421 ttcaagcact tccacatgcg gtttgatgct ggagaaataa ggggcggcaa tacagactcc
     3481 gcgactgaag agcctggtgc caccgggcag gtttttcagg cggttgaagc tggccagtgc
     3541 attcatacaa ttctccgggg tagctgggcc tgaagatgct tttcaggcta tactagaaac
     3601 atattctaga ataatcttct gatatgagcg caaaatcaag tactcgccgc cgggaaatcg
     3661 tgttggcggc attggcctgc ttcaccgagc acggcgttga agcgacctcc atcgacatga
     3721 tccgggaggc gtctggcgcc agtgttggca gcctctacca ccactttggc aacaaggaag
     3781 gccttgctgc cgaggtctat ctggaaggct tgcgggattt tcgccagttc ctggcagagg
     3841 ccctgaggcc cgtgaccgga ctggaggcgg cgacccgtgc catcgtccat gccaatgtgg
     3901 actggatctg tgccaacccg gactgggcgc gttttatctt tggctaccgc atggtgctgg
     3961 cgaaggccaa ccgtgagacc gatctgcagg ctgaggcgca gggtgcccag tctgcgatcg
     4021 ttgcccaact ggagcgactg ctcgccccgg gtcagaacat gccctggccg cccgaggtgt
     4081 atcactcctt ggtgatcggt ccgacccatg attacgcgcg caattggctg gagggccgcc
     4141 gccagcgacc gctggcggag ctgcgcgagg tttttgccga cgcggcctgg aatagcctcg
     4201 gattctccgc gtccgcaaaa aaaggctgat tcatgcccat tggtgcgcac cgacaggcta
     4261 tgcatgacgg ccatgtttct gcacaaagtc gcgtaccgtc accgccattt ccagcggccg
     4321 ggctaacggg gcccagtgtg ctgcatcaat gggggaatgg ctgtagcggg aggctgtttc
     4381 cgggctgact tcaaacacgg cgggtggcag gaaaggatcg ttgaccgcca tcagcacatg
     4441 aacggggacc gggctcgtgt gttttttcgg tagcagggcg cgctgcagca gattggcgcg
     4501 ataaatgccg agataacgaa tggcgtcacc ctccataccg tcgttcttct ggaaggtaat
     4561 gcgcggttcg tagtgtgcga ccagcttgcg gaacatcacg ttgccgagac cgctgcggaa
     4621 cagcagctcc ggtaacagcg gcaaggtgaa gaacgtcatc agactgttgc gcagcagctg
     4681 cccaaaagct tgtagcagat tgcgcggtgt tggcttcaag aagcggcgac gcaggtaaaa
     4741 gccgacgtgg tcaaggctgg gcgacattgt gacaaacgag gcaatgcgct tgatgccttc
     4801 cggctcgcag atggcatccc agccatagat cccgccccag tcatgtccca ccagatgtac
     4861 cttgcgacca ggactgacgg catcaatcac ggcgtacaga tccttgagca gtggtgcgaa
     4921 gtggtaatac ttcgtgccgg aaatgtgcgt ggagtcggca cagccgcgca tgtcgaatgc
     4981 cacgacatga aaatcgtgct gaagggtggt ggccactttc tcccagaaca acgcccggtc
     5041 gggaaagccg tgggccagca ccactgtttc gcgcggatgc tttgcatccg gcttttggcc
     5101 ccaggtgtat accgccagcc tgacttcgcc agactgaaca aacagatccg gttgacgcat
     5161 tgtcattact cctcgcaggt ccgcatcagt tgtgttcacg cgtggcttgt ccgtagacca
     5221 cataaatcat ggccataaac agcaggccgc cactaaaacc cactggcacc acaaagccct
     5281 gcccggcagt gatgtagtcg gccggcacag gcaacaggct catgacctgc agcgtcagga
     5341 aaaacagggc agggaaggga agcaggcaga ggagaaagac aaggctttct ttcatgagtg
     5401 cgtgctggcc gacgcgctgc cccgcgagca gcagcaggct gaagccggtc agcacgaggc
     5461 cgttcaccat gtacatcgtg ccacccgaca gcagcgcctt gccgggcgcc agcccgatgt
     5521 tcgcgccaat aacgcaggag gtgagcccgg cggtgagggc cagccatggt gcggcatgat
     5581 tcacgaaagc gtgcgacggt gcctgttgca acaggccgtt caggcgttgt acgactgccg
     5641 ggaaagccag gctgagactg agtgcaaaca gaacggcaat aaacggatgc gaggccagca
     5701 cactctgttc atgcactacc agcgcagccg gtaccagtgt gtgaccggca tcgagggacg
     5761 acatgccctg ccccgcgatg taaaacgcta cattcacccc cagcaacagc agcgccatca
     5821 cgtgcagtgc cggcaatgcg cgacggaaca gctcggtcgc gtgacctgca attgccgccg
     5881 ccggccgttt tgtcatcggg cgtgcgtatt gccgattcac cagaatcagg ccgtaagcaa
     5941 tgatcaggac ctccggcagc atgattccca cggttaccag actcagggtt tcctgatcga
     6001 tggcgggaaa cagccaggcc agtagtaccc agtccaatcg caacagtggt gccaccatca
     6061 ggcagccaaa agagagggcc atccaagcct ggtgctcgaa gatgcgacgc accttgagcg
     6121 cacgcagcgc catggcaatg gagaccagcg ccagcgcacc gaagatccac aacgcaatcc
     6181 acgccaccag atgagcataa atgtggtgcg gtgccgtcag tgc
//
LOCUS       DASUEG010000168         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_313240, whole genome shotgun sequence.
ACCESSION   DASUEG010000168 DASUEG010000000
VERSION     DASUEG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313240"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFX11_03250"
     CDS             complement(<1..711)
                     /locus_tag="VFX11_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937662.1"
                     /translation="MPIQVLVVDDSAFFRRRIKEILEKNPEIRVCGFADNGRDAVDKA
                     LTLKPDVITMDYEMPLMDGISAVRAIMAKQPTPILMFSSLSFEGARVTLDALDAGALD
                     YLPKSFESMSGDPGESARILQDKVLAITRRGRIMPRPVPPVAAAAPVKPAPSAHSPAP
                     PSPAAPRVAARPLPAATAPSSPAVARKSTVLDTLNGERPRLILIGASTGGPVALQNVL
                     QELPANFPFPILLVQHMPG"
     gene            complement(756..3107)
                     /locus_tag="VFX11_03255"
     CDS             complement(756..3107)
                     /locus_tag="VFX11_03255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX4937663.1"
                     /translation="MSFDADEEILQDFLVEAGEILEVLSEQLVELENRPDDANLLNAI
                     FRGFHTVKGGAGFLQLDTLVELCHAAENVFDLLRNNKLSITSELMDVVLQALDAVNNM
                     FAQVNARAYPEPADPSLMEALHAIIRGEAPGAAHAAAPEPEEEAPAPAAKKSAAAGKT
                     QGDITDEEFEQLLDALADEKAPAKPAAPADTGVDLFGEDEEVKAAAGGGDEITEAEFE
                     ALLDQLHGKGKPVGAAKPAASTAAPAKAAGGEEISDDEFEALLDELHGKGKHGGLPKS
                     ADESPAPKAKGKPGSEDLISDDEFESLLDELHGKGKGPTHKGGSETPASTVAAAKPAA
                     PKAPASEKPAAKPAARPEPKADAPAAARPAAKPAAAAAFDDEDDERKAARPGAAGAAA
                     PKEAAENTVRVDTKRLDDIMNMVGELVLVRNRLVRLGLRSADEDLAKAVANLDVVTGD
                     LQTAVMKTRMQPIKKVFGRFPRVVRDLARNLKKEINLELEGEETDLDKNLVEALADPL
                     VHLVRNSVDHGIEAPDVREKMGKHRTGRVVLAAQQEGDHIVLSITDDGGGMDPAVLKR
                     KAVEKGLMDTETAERLSDTEAFNLIFHPGFSTKDTISDVSGRGVGMDVVKTKISQLNG
                     TIDVKSERGVGTRIAIKVPLTLAIMPTLMVMLGRQAFALPLVNVNEIFHLDLSRTNVV
                     DGQEVVVVREKALPLFHLKRWLVRGAAASEREAHVVIVSVGTQKVGFVVDQLIGQEEV
                     VIKPLGKMLHGTPGMAGATITGDGRIALILDIPSMLKAYAHRG"
     gene            complement(3120..3896)
                     /locus_tag="VFX11_03260"
     CDS             complement(3120..3896)
                     /locus_tag="VFX11_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX4937664.1"
                     /translation="MTERAQQEPLVEYAKALLAQLESGDAVEAMQTIASLHKARDQYL
                     FQEVGKLTRSLHESIKNFSIDASKAGSAMHDEMSRMQDASQRLNYVIEKTEQAANKTM
                     DMVESTIPVSSQLGDRARELKPEWQRLMRREMKPEEFRLLTREMDNFLDMAAHETSKI
                     DSNLSNILLAQDFQDLTGQVIKRVIQLVQEVEESLVHLVRMAGAIDEITGMKHDIPEK
                     IVDTSQPNIGPEGPIINPDKRNDVVAGQDDVDALLSSLGF"
     gene            complement(3929..4315)
                     /gene="cheY"
                     /locus_tag="VFX11_03265"
     CDS             complement(3929..4315)
                     /gene="cheY"
                     /locus_tag="VFX11_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEX4937665.1"
                     /translation="MNKNMKILIVDDFSTMRRIIKNLLRDLGFTNTQEADDGSSALPL
                     LKASDFDFLITDWNMPGMTGIDLLKAVRSDDKLKSLPVLMVTAEAKREQIIEAAQAGV
                     NGYVVKPFTAQALKEKIEKIFERVDA"
     gene            complement(4425..>4823)
                     /locus_tag="VFX11_03270"
     CDS             complement(4425..>4823)
                     /locus_tag="VFX11_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4937666.1"
                     /translation="EHRTGRDASDREVAAELNISLDDYHGMLQDSMATRLFSIDEMES
                     PDDHIDLSAQDRGSDNPLDELTGDNFRQHLAAAIETLSEREQLVLSLYYEQELNLKEI
                     GEVLGVSESRVSQIHSQAAHRLRARLREWL"
BASE COUNT          856 a         1484 c         1367 g         1116 t
ORIGIN      
        1 gccgggcatg tgctgcacca gcaaaatcgg gaacggaaaa ttggcgggca gttcctgcag
       61 gacattctgc agtgccaccg gaccgccagt ggacgcaccg atcagaatca ggcgcggacg
      121 ctcgccattg agcgtatcca gcacggtgga tttgcgcgcc actgccggcg acgacggggc
      181 ggttgcagcg ggcagcggtc gcgctgcaac ccgcggcgct gctggtgatg gcggcgcggg
      241 cgaatgcgcg gacggtgctg gcttaactgg cgctgcagcg gcaaccggtg gcacaggccg
      301 cggcatgatc cggccgcgtc gcgtaatcgc aagcaccttg tcctgcagga tgcgggcgct
      361 ttccccggga tcgccagaca tggattcgaa acttttggga aggtaatcca gcgccccggc
      421 gtccagcgcg tcgagcgtaa cgcgcgcacc ttcaaaactc agggatgaaa acatcaggat
      481 aggcgtgggc tgcttcgcca tgatggcgcg cacggcagag atgccatcca tcaacggcat
      541 ctcgtaatcc atggtgatga catccggctt caacgtcagc gccttgtcca ccgcatcacg
      601 gccgttgtcg gcaaaaccgc agacccggat ttcaggattt ttttccagaa tttcctttat
      661 ccgccggcga aaaaaagcgg aatcatcgac cacaaggacc tggatcggca tctgtcatct
      721 ccccacgcgc tgccggactt cactcaacac cggcttcatc ctcgatgcgc gtacgctttc
      781 aacatgctgg gaatatccag gatcagcgca attcggccgt cgcccgtgat ggtggcgccg
      841 gccatacccg gcgtgccatg cagcatcttc cccagcggtt tgatcacaac ttcttcctgg
      901 ccaataagct ggtccaccac aaagccgact ttctgtgtac ccacgctgac gatcaccaca
      961 tgggcctcgc gttcgctggc cgctgctcca cgcaccagcc agcgtttgag atgaaacagc
     1021 ggcagcgctt tttcccgcac caccaccact tcctgtccat ccaccacatt ggtacgtgac
     1081 agatccagat gaaaaatttc gttcacattc accagcggca gcgcgaacgc ctggcggccc
     1141 agcatgacca tcagcgtcgg catgatggcg agagtgagtg gcaccttgat ggcgatacgg
     1201 gtgcccactc ctctttcgga tttgacgtcg atggtgccat tgagctggct gatcttggtt
     1261 ttcaccacgt ccatgccgac accacggccc gacacgtcgg agatggtgtc cttggtggaa
     1321 aaacccgggt gaaaaatcag gttgaaggct tcggtgtcgc tgaggcgctc ggcggtttcc
     1381 gtgtccatca gccctttttc cacggccttg cgtttgagta ccgccgggtc catgccgccg
     1441 ccatcgtcgg tgatggacag cacaatatga tcgccttcct gctgcgcggc cagtacaacg
     1501 cggccagtgc ggtgtttgcc cattttttcc cgcacatccg gtgcctcgat cccatggtcg
     1561 acgctgttgc gtaccaggtg caccagcgga tcggccagcg cttccaccag attcttgtcg
     1621 agatcggttt cctcgccttc cagttccaga ttgatttctt tcttgaggtt gcgcgccaga
     1681 tcgcgtacca cgcgcgggaa gcggccgaat actttcttga tcggctgcat gcgggttttc
     1741 atcacggcgg tttgcagatc gccggtgact acgtccaggt ttgccaccgc tttcgccaga
     1801 tcctcgtcag cactgcgcag acccagccgc accaggcggt tgcgcaccag caccagctcg
     1861 cccaccatgt tcatgatgtc gtccaggcgt ttggtgtcga cgcgcacggt gttttcggct
     1921 gcttccttgg gtgccgccgc accggcagca ccaggccggg ccgccttgcg ctcatcatct
     1981 tcatcatcga aagccgctgc cgcagcgggt ttggccgccg ggcgcgctgc agcaggtgca
     2041 tccgctttcg gttccggacg ggcggccggc ttggccgccg gtttttcgct ggcaggcgct
     2101 ttgggtgctg ccggtttcgc cgccgctacg gtgctggcag gggtttcgct cccacccttg
     2161 tgggttgggc ccttgccctt gccatgcagt tcgtccagca gggattcaaa ttcatcatcg
     2221 gaaatcaggt cttcgctacc gggcttgcct tttgctttgg gcgccggtga ttcatcggca
     2281 gactttggca ggccaccatg tttgcccttg ccatgcagtt catccagcaa cgcctcgaat
     2341 tcgtcgtcgg aaatttcctc gccacccgcc gctttggcag gcgccgcagt agatgccgcc
     2401 ggtttggctg cgcccactgg tttgcccttg ccatgcaact ggtccagcag agcttcgaat
     2461 tcggcttccg tgatttcgtc tccgccgccg gcggcggcct tcacttcctc gtcttcacca
     2521 aaaagatcga ctccggtgtc cgccggtgca gcgggtttcg ccggcgcctt ttcatccgcc
     2581 agtgcgtcga gcagctgctc aaattcctcg tccgtgatat cgccctgcgt ttttccggcg
     2641 gcggctgatt ttttcgctgc gggcgccggt gcttcctctt caggttcagg cgcggcagca
     2701 tgtgcagcgc caggtgcctc gccgcggatg atggcatgca gcgcttccat cagagaagga
     2761 tcggcaggtt cgggataggc gcgcgcattc acctgcgcaa acatgttgtt cactgcatcg
     2821 agggcctgca gcaccacatc catcagttcg gaggtgatcg acagcttgtt gttgcgcagc
     2881 aggtcaaaca cgttttcggc agcgtggcag agttccacca gtgtatccag ctgcagaaag
     2941 ccggcaccgc cttttacggt gtggaagcca cgaaagatgg cgttgagcag attggcgtcg
     3001 tcgggccggt tttccagttc caccagctgt tccgataaca cttcgaggat ttcgccggct
     3061 tcaaccagaa aatcctgcag aatctcttca tcagcgtcga aactcatccc ctgactccat
     3121 cagaaaccaa gactggacag cagcgcatcc acatcatcct gaccagccac cacgtcgttg
     3181 cgcttgtccg gattgatgat gggtccttcc ggaccgatgt tgggctgcga ggtatccact
     3241 atcttttcgg gtatatcgtg tttcattccg gtgatctcgt cgatggcgcc tgccatgcga
     3301 accagatgca ccaggctttc ttccacttcc tgcaccagct ggatcacgcg cttgatcacc
     3361 tggccggtga gatcctggaa atcctgcgcc agcagaatat tggaaagatt gctgtcgatc
     3421 tttgaggttt catgcgccgc catatcaagg aaattgtcca tttcccgcgt cagcagccgg
     3481 aattcttccg gcttcatttc ccgccgcatc aggcgttgcc attccggttt caattcgcgg
     3541 gcacggtcac ccagctgcga tgagaccgga attgtgcttt ccaccatgtc catggttttg
     3601 ttcgcggcct gttcggtttt ttcgatcacg tagttcaggc gctgcgaggc atcctgcatg
     3661 cgcgacatct catcgtgcat ggcgctgccg gccttgctgg catcaatgga aaaattcttg
     3721 atcgattcgt gcaggctgcg ggtaagtttg cctacttcct gaaacaggta ctggtcccgc
     3781 gccttgtgca gactggcgat ggtttgcatg gcttcgacag catcgccact ttccagttgc
     3841 gccagcaggg ccttcgcgta ctcgaccagt ggttcctgtt gcgctcgttc cgtcatgctt
     3901 gtctcacatc cgttcggctg tcgctggctc aggcatccac gcgttcaaag atcttctcaa
     3961 ttttttcttt cagcgcctgc gcggtgaaag gtttgaccac atagccattg acgccggcct
     4021 gggccgcctc gatgatctgc tcgcgcttgg cttcagccgt caccatcagc accggcaggc
     4081 tcttcagctt gtcgtcggaa cgcacagcct tgagcaggtc gatgccggtc atgccgggca
     4141 tattccaatc ggtaataaga aagtcaaaat ctgacgcctt cagcaagggc agcgccgaac
     4201 ttccgtcatc cgcttcctgg gtgttggtaa accccagatc gcgcagcagg tttttgatga
     4261 tgcgcctcat cgtggagaag tcgtcgacga tgagaatttt catgttcttg ttcaaggctg
     4321 cctccattga accgttacaa ctacacagat ttgtttaggg aagctttccc gctagaaatt
     4381 aagtctagta gcagaatccg ccgtatccag tttttgcagc ggaatcaaag ccactcacgc
     4441 agacgggcac gcaggcggtg ggctgcctgg ctgtggatct ggctgacgcg ggactcgctg
     4501 acccccagca cttcgccgat ttccttgagg ttgagctcct gctcgtaata cagggacagt
     4561 accagctgct cccgttctga cagggtttcg atggcagccg ccaggtgctg gcggaagttg
     4621 tcgccagtaa gttcgtccag gggattgtcg gaaccccggt cctgcgcgga cagatcgatg
     4681 tgatcgtccg gggattccat ttcgtcgata ctgaacagac gggtggccat gctgtcctgc
     4741 agcatgccgt ggtaatcgtc caggctgatg ttcagttctg cggccacctc acgatcactg
     4801 gcatcgcggc cagtgcggtg ctc
//
LOCUS       DASUEG010000169         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1970876, whole genome shotgun sequence.
ACCESSION   DASUEG010000169 DASUEG010000000
VERSION     DASUEG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970876"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFX11_03275"
     CDS             <1..992
                     /locus_tag="VFX11_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4937667.1"
                     /translation="RMPWSSRKRLSKGRFTIPVGGDGGVQNPNLPPPSRPEEEDVPIR
                     ADQRAGIPYPEWNVWTKRFLPHHVAVLERKHPPSPQQSPQASANLRRWFEAHTHRAMK
                     NRLEDGSDLDVDRYINHYADVLSGTASDARVFRDLLPAARDVTTALLLDGSSSLGVHQ
                     GRVFQLELACADALSQAMAQARERHGLFVFSGNTRHRVDVICLKDFTDRRTVIPGKLG
                     LSVGGYTRLGAPLRHMTSRLLEQPSERRLLIVIGDGLMSDEGYEGHYAWADVAHAVEE
                     AEEAGVFPYYIGIGPARVDPLPDVFGAQRSTRIRRIEELPRVLAHVHQQLVAA"
     gene            1015..1854
                     /locus_tag="VFX11_03280"
     CDS             1015..1854
                     /locus_tag="VFX11_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX4937668.1"
                     /translation="MNIKVDGLQPAASGSDTYHVNCNEEQLFRLAYSQRLPVMLTGPT
                     GCGKTRFVEYMGEKLGRPVITISCHDDLTSSDLVGRFMVTGGDVVWNDGPLTRSVKQG
                     AICYLDEVVEARHDSLAVLHSLTDHRRALYLDRAGEVIQAPDTFMLVCSYNPAYRSSL
                     KELKPSFRQRFMTLAMNYLPADREAEVLVTESNIPLSVAQRLVRFAVNIRNADAAFHF
                     EPPSTRVLVTAAKLIAAGATEMEAAEVCILAPLSSDGAITDGLRELAAASLSTHAGTA
                     NKQ"
     gene            2019..>2227
                     /locus_tag="VFX11_03285"
     CDS             2019..>2227
                     /locus_tag="VFX11_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937669.1"
                     /translation="MNTNTLTMGGMNDLDDHHERSRKAQWQADKWMMAGALFLGTAVF
                     GLIGFPLFLRGLALQIKAQRQGLSVR"
BASE COUNT          457 a          661 c          656 g          453 t
ORIGIN      
        1 gtcgcatgcc ctggtcgtca agaaaacgct tgtcgaaggg ccggttcacg attccggtcg
       61 gcggcgacgg cggcgtacag aatccgaatc tgccaccgcc cagtcgcccg gaagaggaag
      121 atgttccgat tcgcgcggac cagcgcgccg gcattcccta tccggaatgg aatgtctgga
      181 caaagcgttt tctgcctcat catgtcgccg tactggaacg aaagcatccg ccgtcaccac
      241 agcagtcgcc gcaagcgtcg gcgaatctgc gccgctggtt cgaagcgcac acgcatcggg
      301 ccatgaaaaa ccggctggaa gatggttccg atcttgatgt ggaccgttac atcaatcatt
      361 acgccgatgt gttgagtggc acagcgtccg atgcgcgcgt ctttcgggat ttgctgccgg
      421 ccgcacgcga tgtcaccacg gcgctgctgc tggatggcag ctcgtcgctg ggtgtgcatc
      481 agggtcgcgt tttccagctt gaacttgcct gtgccgacgc gctgtcccag gcgatggcac
      541 aggcgcgtga acgtcacggc ctgtttgtgt tcagcggcaa cacccggcat cgggtcgatg
      601 tgatctgcct gaaggatttt accgatcgcc ggactgtgat tcccggcaaa ctcggtcttt
      661 ccgtcggcgg ctacacacga ctgggcgcgc cgttgcgaca catgaccagc cgcctgctgg
      721 agcaaccatc cgaacggcgc ctgctgattg tgattggcga cggcctgatg tccgacgaag
      781 gttacgaagg tcattacgcc tgggccgatg tcgcgcatgc agtggaagag gctgaagagg
      841 ccggcgtgtt cccctactac atcggcatcg gccccgcccg ggttgatccc ttgccggatg
      901 tgttcggcgc acaacgctcg acccgcattc gtcgcatcga agaactgccc cgcgttctcg
      961 ctcatgtcca tcaacaactg gtggcagcgt gatgattttt actggatgca caccatgaat
     1021 ataaaagttg acgggctaca gccagcagcc agcggttccg atacttatca cgtcaactgc
     1081 aatgaagagc agctgttccg gcttgcatat tcgcaacgac tgcctgtaat gctgaccgga
     1141 ccaaccggat gcggtaaaac ccgctttgtc gaatacatgg gtgaaaagct cgggcgtccg
     1201 gtgatcacca tcagctgtca cgacgatctg accagttccg atctggtggg gcggtttatg
     1261 gtcaccggcg gcgacgtggt ctggaacgac gggccgctga cccgctcagt gaaacagggg
     1321 gccatctgct atctcgatga ggtggtggaa gcacgacacg attcgctggc cgtgctgcac
     1381 tcgctcacgg atcaccggcg tgcactgtat ctcgatcgcg ccggggaagt cattcaggcg
     1441 ccggacacgt tcatgctggt gtgctcctac aaccccgctt accgcagttc gctcaaggaa
     1501 ttgaaacctt ctttccgcca gcgcttcatg acgctggcca tgaattatct gccagccgat
     1561 cgcgaggccg aagtgctggt aaccgaatcc aatattccgt tatcggtcgc gcagcgcctg
     1621 gtgcgctttg ccgtcaacat ccgtaacgct gacgctgcgt ttcatttcga accgccctcc
     1681 acccgcgtgc tggtaaccgc cgccaaactg attgcagcgg gcgccaccga aatggaagcg
     1741 gcggaagtct gcattcttgc accgctgagc agtgatggtg ctatcaccga tggcctgcgg
     1801 gaactggcgg cagccagcct gtccacgcac gcaggcacag ccaataaaca atgacaacaa
     1861 gaggtctccg caatgaagga acagccaatc agtgaacatg accgtcgacg ccgcaaagcg
     1921 cttatcgctt tccagatagt ctgctacggc tatctgctga cgatgttttt gatccagatg
     1981 tatatgcact ccgtgcgcga ctggtgaggg acgaaacgat gaacaccaac acactgacca
     2041 tgggcgggat gaacgatctc gacgaccatc acgagcgcag ccgaaaggca cagtggcagg
     2101 cggacaaatg gatgatggcg ggtgccctgt ttctgggaac cgcggtattc ggcctgattg
     2161 gctttcccct gttcctgcgc ggactggcac tgcaaatcaa ggcgcaacgg caagggcttt
     2221 cagtacg
//
LOCUS       DASUEG010000170         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1853623, whole genome shotgun sequence.
ACCESSION   DASUEG010000170 DASUEG010000000
VERSION     DASUEG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853623"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2141
                     /locus_tag="VFX11_03290"
     CDS             <1..>2141
                     /locus_tag="VFX11_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4937670.1"
                     /translation="VLPGVFIGAVVANAIITLWQGHDLAYRQLLLAVMLAIGATLQAA
                     ISAALILRNQPQQGLLDDERAMIRFVLLAGPVGCLISATNGATQLSLFGVIHWSLWSS
                     NWLTWWIGDAVGTLLVTPALLKILTPQLRRTSVHPLVISAPSLIMLALVVGSFYYVRN
                     LGEEKRYSSTAEYGSKIAAEIRWQLQEAHFALKAMQGLYYSSDYITSEEFDRFSRHLQ
                     LTMPGLQALEWAPKVTGDQRAAVEARMRQQGYSNFEFIHRTPNGELIAAEPRPVYFPI
                     LYVYPYETNKRVHGLDIHQLSYRRDEILDAIQNNLTVFSSPMRLVQGGKDFSYIVFTP
                     VYQLDRLTADLSSGGSNKKSAGMDEVLGLVQVVVRFRDIIDQITERLYLDKVSLSVYD
                     VNDRSRPVLLWGEEERTSRYRWSTEFPLHNRLLQLHIEPTPQMLKEVSQWQTYGLLIG
                     GLLYVAMLEFMLLSLSSRHGIIERQVAEKTQELERAKESAELANRAKTEFPANMSHEL
                     RTPLNGIIGFTRRILKNESASLSPRAQESLEVVTRNSLHLLTLINDLLDLSKVEAGKF
                     MLEFSSFDLVEMLREVEAQFALEATSKGLHWAVNMPPQPLQITADRQRLMQVLINLVS
                     NAIKFTQQGEVSLAVAERSINGVRGIEFSVRDTGMGIAREDIPRLFNKFEQLKNRNQG
                     SLRGTGLGLALAREIITLHQGTVRVESTPGS"
BASE COUNT          449 a          632 c          650 g          410 t
ORIGIN      
        1 gggtactgcc cggcgtgttc atcggcgcag tggtggccaa tgcgatcatc accctgtggc
       61 aagggcatga cctggcctac cgccagctgc tgctggccgt gatgctggcg attggcgcca
      121 ccctgcaggc cgcgatcagc gcggcgctga tactgcgcaa ccagccgcaa caggggctgc
      181 tggacgacga acgcgccatg atccgtttcg tgctgctggc cggtcccgtc ggctgtctga
      241 tctcagctac caacggcgcc acccagctga gcctgttcgg tgtcattcac tggtcgttgt
      301 ggtccagcaa ctggttgacc tggtggatcg gtgacgcggt ggggacgctg ctggtcacac
      361 cggcactgct gaaaatcctg acgccacagc tgcgccggac atcggtgcat ccactggtga
      421 tcagcgcgcc gtcgctgatc atgctggcat tggtagtggg atcattttat tacgtgcgga
      481 atctgggcga agaaaagcgt tacagttcca ccgccgaata cggcagcaag attgcggcag
      541 agatccgctg gcaattgcag gaagcccatt ttgcgctgaa agcgatgcag ggcctgtact
      601 acagcagcga ttacataacg tccgaagaat tcgaccgctt ttcacgccac cttcagctca
      661 ccatgcctgg tctgcaggcg ctggaatggg cacccaaggt aaccggcgat cagcgtgcgg
      721 cggtggaagc ccgcatgcgc cagcaggggt attccaattt cgaattcatt catcgcactc
      781 ccaacggtga actgattgcg gcggaaccgc gccccgtgta tttccccatc ctgtatgtgt
      841 atccctacga aaccaacaag cgggtacatg ggctggatat ccaccagcta tcctaccggc
      901 gggacgaaat cctggatgcc atccagaata atctgacggt gttcagcagc ccgatgcggc
      961 tggtgcaagg tggcaaggat ttctcgtaca tcgttttcac gccggtttac caactcgacc
     1021 gcctgactgc cgacctgtcc agtggcggca gcaacaagaa gtccgctggc atggacgagg
     1081 tgctggggct ggtgcaggtg gtggtgcgct ttcgcgatat catcgaccag attaccgagc
     1141 gcctctacct ggacaaggtg agcctgtcgg tttacgacgt caatgaccgt tcccgtccgg
     1201 tgctgctgtg gggcgaggaa gaacgcacgt cccgctatcg ctggagcacc gagttcccgc
     1261 tgcataaccg cctgttgcag ctgcatatcg aacccacacc gcaaatgctc aaggaggtca
     1321 gccagtggca gacctatggc ctgctgattg gcggcctgct gtatgtggcg atgctggaat
     1381 tcatgttgct gtcgctgtcg agccggcacg gcatcatcga acgccaggtg gcggaaaaaa
     1441 cccaggaact ggagcgcgcc aaggaatcgg cagaactggc caaccgggcc aaaaccgaat
     1501 ttccggccaa catgtcgcac gagctgcgca caccactcaa cggcatcatc ggattcaccc
     1561 gtcgcatcct gaaaaacgag agcgccagcc tgtcaccgcg ggcgcaggaa tccctggaag
     1621 tggtcacgcg caacagcctg catctgctta ccctcatcaa cgatctgctg gatctgtcca
     1681 aggtggaagc aggcaaattc atgctggagt tctccagctt cgacctggtg gaaatgttgc
     1741 gggaagtgga agcccaattc gcgctggagg ccaccagcaa gggcctgcac tgggcggtga
     1801 acatgccgcc acagccgctg cagattaccg ccgaccgaca gcgcctgatg caggtgttga
     1861 tcaatctggt gagcaatgcg atcaagttca cccaacaggg cgaagtgtct ctggccgtgg
     1921 cggagcgcag catcaacggc gtcaggggaa tcgaattctc ggtgcgtgat accggcatgg
     1981 gcatcgcgcg ggaggacatt ccgcgcctgt tcaacaaatt cgagcagctg aaaaaccgca
     2041 atcagggttc gctgcgggga accgggctcg ggctggcact cgctagggaa atcatcaccc
     2101 tgcaccaggg tactgtgcgg gtggaaagca ccccgggatc a
//
LOCUS       DASUEG010000171         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_846187, whole genome shotgun sequence.
ACCESSION   DASUEG010000171 DASUEG010000000
VERSION     DASUEG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846187"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1396)
                     /locus_tag="VFX11_03295"
     CDS             complement(212..1396)
                     /locus_tag="VFX11_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4937671.1"
                     /translation="MIKGQNNSGAGNAGLRHEQAPLSYSVCSGKPPFMPGDKLDNSTK
                     LPGKIFRYIHFILAISVAGCSAAGDKGLESSSRDSSLKWITDTSVPTHAENGVAKLQF
                     IGAGGFYLSLPQGGVLVDPFYSNPGLLSLASLRTLVPDTQTIDRYLPPPDDINAVLIG
                     HVHFDHALDLPYIASKLAESARIYGSATLKNSLATAIASDRLIDVEPGMSVDGRGGQW
                     LMLAPHLRVFPIKSEHAPHIGSWVFGSGNVEKPLDHVPEDVLDWQAGVNISYLLDLLD
                     DEKRPVYRVFIQTSASSSPNGLAPNSVLDDGKAVDMAVLCAANYDKMEHYPEYLLERM
                     SPQKVVLVHWEKFWTPYEPGQASPLPGLNIDNLYRRINAVLPQAEVSILQRGAILSWS
                     LN"
     gene            complement(1377..2345)
                     /locus_tag="VFX11_03300"
     CDS             complement(1377..2345)
                     /locus_tag="VFX11_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEX4937672.1"
                     /translation="MLSTYDASPKAAASSTPPPDSVALAMLARQETVPSITTGLEQLP
                     PSLRGTEVDGVLRVDNNGNLVIDHDIRRVFDYFLSTLGEEPLPVIELRLRAYIRHQLP
                     ATAAQQAEQLLEDYLALNRALSDLQAPADGDTSNAPDTATLRDRLLSIQDLRRQYLQA
                     DVVDAFYADDDALDQLALGRMEIVEDRTLSATQKTQALNALEQSLPQHLQGAIKELNR
                     HQQMSTLTARLRRQGGTDAEVYQLRESFFGAEAAERLAQLDQSRQQWQARATLWLDER
                     DSLIARQDIDEADRQRQLDLRRRDLFEPAELRRIESIEHLHDQRSK"
     gene            complement(2409..>3104)
                     /locus_tag="VFX11_03305"
     CDS             complement(2409..>3104)
                     /locus_tag="VFX11_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX4937673.1"
                     /translation="QLLDYVDEVLAITGAEKVNLIGHSLGALTVRYVAAVAPEKVASV
                     SAIGSLTLGSPVGDWAQEVFKMPVIGEPIQTIAEAVGNLMGYVLNTLEGEKFDQDAHS
                     LFITLSGAGAADYSARFPQGVPETRCGEGDHEVNGVRYYSWGGTKVFTHVLDPFDYLA
                     AVTSLFFKGVDDGTSDGLVGRCSSHLGMVIRDNYRMNHLDEVNQVLGLRDPYTDPVAI
                     FRQQANRLKLQGL"
BASE COUNT          689 a          878 c          855 g          682 t
ORIGIN      
        1 aggtgtggtg cggttacacc ggggcaggat tgagatactg gacagtgaac agttgcagaa
       61 aataatccag actgacgaag aaaactacta ggcgcaaatc gcatttgatt cgaccccttt
      121 tgccgtcaat gaaccgctga acaaattcca cggtttggca gcgaccgacc tgcgcaagga
      181 aatatcgcca gcacccactg catttgcggc ctcaattgag gctccaactc aaaatggctc
      241 cgcgttgtaa tatgctgacc tcggcttgcg gcaggacggc gttgattcgc cgatagagat
      301 tatcgatatt caggccgggt agcggagagg cttgcccggg ttcgtaaggt gtccagaact
      361 tctcccaatg cacgagtacc actttttgcg gactcatcct ttcgagcaaa tattccgggt
      421 aatgttccat cttgtcgtaa ttcgccgcgc acagtaccgc catgtcgacc gctttcccgt
      481 catccagcac gctattcggt gccaggccgt tgggcgagga cgaggcggac gtctgaatga
      541 atacccgata gacgggcctt ttctcatcat ccagcagatc aagcagatag ctgatattca
      601 cgccggcctg ccaatcgaga acatcctcgg gcacatgatc caaaggtttt tcgacattac
      661 ccgaaccgaa cacccaggac ccgatatgag gcgcgtgttc cgacttgatc ggaaataccc
      721 gcaaatgtgg ggcgagcata agccactgcc caccgcgccc atcgacggac atgccgggtt
      781 caacatcaat cagtctgtct gacgcaattg cggtggcgag actgttcttt aacgtggcgc
      841 tgccatagat tctggcgctt tcagccagtt tgctggcgat gtagggcaaa tcaagtgcgt
      901 ggtcaaaatg cacatggcca atcagtacag catttatatc gtcgggcggt ggcaggtagc
      961 ggtcaatggt ttgcgtgtct ggaaccagcg ttctcaaact ggccagggac aataaacccg
     1021 ggtttgagta aaaggggtcc accagcacgc caccctgtgg aagtgagaga tagaacccgc
     1081 ccgcaccgat gaactgcaac ttcgccacgc cattctctgc gtgcgtcggc acgctggtgt
     1141 cggtgatcca tttcaaggag gaatccctgc tggatgactc aaggcctttg tcgccggctg
     1201 cactgcaacc tgccactgaa atggcaagaa taaaatgtat atacctgaaa atttttccag
     1261 ggagtttcgt cgaattatcg agcttgtcgc cgggcataaa aggaggtttt ccagaacata
     1321 cagaatacga tagcggtgct tgttcatgcc tgaggccagc gttgcctgcg ccggaattat
     1381 tttgaccttt gatcatgcaa gtgttcgatg gactcgatgc gccgcaattc ggcaggctcg
     1441 aagagatcgc gccgccgcaa gtcaagctgg cgctgacggt cggcttcgtc gatatcctgc
     1501 ctggcgatca agctgtcgcg ctcatccagc cagagcgtag cgcgtgcttg ccactgctgt
     1561 cgcgattgat caagttgcgc gagccgctct gcggcttcgg ccccaaaaaa actttcacgc
     1621 aactggtaga cctcggcgtc agtgccaccc tggcgacgca gacgcgctgt cagggtgctc
     1681 atttgctgat gtcggttgag ttccttgatg gcaccctgca gatgctgagg cagcgattgt
     1741 tcgagtgcat tcagggcctg cgttttctgt gttgccgaca gtgtccggtc ttccactatt
     1801 tccatgcggc ccagggcgag ctgatccagg gcatcgtcat cggcgtagaa ggcgtcaaca
     1861 acatctgcct gcaggtattg ccgacgaaga tcctggatag acaagagccg gtcacgcagc
     1921 gtagccgtgt ccggtgcgtt gctggtatcg ccatctgcgg gcgcctgcaa gtctgacagt
     1981 gccctgttca gtgccagata gtcctccagc agttgctccg cctgttgcgc tgcggttgcg
     2041 ggtaattgat gccggatata ggcccgcagc cgcagctcga tgacaggtaa cggctcctcg
     2101 cccagtgtcg aaaggaaata atcaaacacg cgacgaatgt catggtcgat gacgagattt
     2161 ccattgttgt cgacacgcag tacgccatcc acttccgtgc cccgcagtga agggggcagt
     2221 tgctcaagcc cggtggtgat cgaaggcacc gtttcctgcc gggcaagcat cgcgagagca
     2281 accgaatccg gcggaggcgt tgatgacgcc gcggcttttg gcgatgcgtc gtaggtggac
     2341 agcaaccgga tgcctgcgaa catgacgatc aacaggacga acaaggctgc cgtttttttc
     2401 atggcggctt acagtccctg gagtttcagg cggttggcct gctggcggaa aatcgcgacc
     2461 ggatcagtat aaggatcgcg cagccccagc acctgattga cttcatccag atgattcatc
     2521 cgatagttgt cgcgaatcac catgcccaga tgactgctgc aacgcccaac caggccatcg
     2581 cttgtgccat cgtcgacacc tttgaaaaac agtgaagtaa cggccgccag gtaatcaaat
     2641 ggatcaagca cgtgcgtaaa caccttggtt ccgccccagg agtaatagcg cacaccgttg
     2701 acctcgtggt ctccctctcc gcagcgggtc tcgggaacgc cttgtggaaa acgggcgctg
     2761 tagtccgctg cgccggcacc cgacaacgta atgaaaagac tgtgagcatc ctgatcgaac
     2821 ttttcacctt ccagggtatt caacacgtag cccatcaagt tgccgacagc ttccgcaatc
     2881 gtttgaattg gctcgccaat aacgggcatc ttgaatactt cttgggccca atcccccacc
     2941 ggggatccca atgtcaggga gcctatggcc gacacggatg ccactttttc cggcgctact
     3001 gccgccacat agcgcaccgt cagtgcgccc aggctgtgtc ctatcagatt gactttttcg
     3061 gcgccagtaa tcgccaacac ctcgtccaca taatccagca gctg
//
LOCUS       DASUEG010000172         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1308176, whole genome shotgun sequence.
ACCESSION   DASUEG010000172 DASUEG010000000
VERSION     DASUEG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308176"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFX11_03310"
     CDS             <1..253
                     /locus_tag="VFX11_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4937674.1"
                     /translation="HQPHSAGNGHMAAFIAPDRKVVDQAYALALAKGGLSEGAPGLRS
                     EYHANYYGAYFRDPDGNKLCVVCHLPEAESENRQNNNLG"
     gene            778..1152
                     /locus_tag="VFX11_03315"
     CDS             778..1152
                     /locus_tag="VFX11_03315"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX4937675.1"
                     /translation="MAGKRPVTIDEYIQAAPLEGQPHLRRLYALLKSVAPQAEEAIKW
                     GTPFFVEPRFLFAFSAHKAHLNFTPMPTGLEPFRKELEKHKTTKGSLQVPYSKPLPED
                     LIRRMAEHSLRVVSEREDDAFW"
     gene            1208..1648
                     /locus_tag="VFX11_03320"
     CDS             1208..1648
                     /locus_tag="VFX11_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4937676.1"
                     /translation="MIEAVNSDNLHEILPLIHRYQEFYEVSAIDDDRNYQFFSQFGLH
                     SPMGCQFLARVEGVAVGFATVYFTYSSTAVAKVGVLNDLYTLADHRGKGVGRALIEHC
                     LAYAVENGAIRLQWVTATDNEVAQRLYDSLAVNKKPWLFYSYKG"
     gene            1746..2144
                     /locus_tag="VFX11_03325"
     CDS             1746..2144
                     /locus_tag="VFX11_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX4937677.1"
                     /translation="MLLIETEIRESEIHGIGLFCVEPVAKGTKVWEFNPLFDLTLREA
                     ELGSLPPPMHSFLRKYAYRSLETGELIVNLDLSRHMNHSDEPNLVSDAQSNYFAVCDL
                     APGTELTCDYRAFSVEGCSDFLDAVLDGAQ"
BASE COUNT          546 a          572 c          720 g          551 t
ORIGIN      
        1 tcatcagccg cattcggcag gcaatggtca tatggctgct tttatcgcgc cagaccgaaa
       61 ggtcgtcgat caggcttatg cactcgcatt ggcaaagggc ggtttgtctg aaggtgctcc
      121 aggtttgcga tctgagtacc atgcaaatta ctacggtgcc tactttcgcg atcctgacgg
      181 caataaactt tgtgtagtgt gtcatttgcc tgaggctgag agtgaaaata ggcaaaacaa
      241 taatctggga tagcaaggaa ctgcatgatc agtacgccaa gaaagtgagg gcatccggat
      301 cgttcgataa aatggcggcg tttttggccg ggccgccgga gtcggagttg ttcatcatca
      361 aatagtggtt gggctggaaa tggaaattgc acatgcctaa gagccacaaa accggaacaa
      421 atcccgaggt tgacgcatgt ctgaaaaagc caagcaggga aggaagaatt cgaaaagcca
      481 ggagagatcg cgcttgagcg tcagcaggct gaagagctga agtggggcac gccctgctac
      541 gaattccaat aaagttgcat catcatcctg cagggcttcc tggaaacttg cgcggtgctg
      601 ctttccaagg gtgcgttgtt agaagatccc aacggtgtta tggaaatgcc cggcgagaat
      661 taacagacag agtgcccgat tccgttcatc agcgcacgct ggatgaaatg ccggccattg
      721 aaaatggcct accagccctg gcaagggatt gagtgaccag taaaggaggc aaagacgatg
      781 gcaggcaagc gtcccgtgac gattgacgaa tatatccagg cggccccgct ggagggccag
      841 ccgcacttgc gccggctgta tgcactgctc aagagtgtcg cgccgcaggc cgaggaagcg
      901 atcaagtggg gaacaccttt cttcgtggag ccccggttcc tgttcgcgtt ctccgcacac
      961 aaggcccatc tgaatttcac gcccatgcca acggggctgg agccatttcg caaggaactg
     1021 gaaaagcaca agaccaccaa aggctcactg caggtgcctt acagcaagcc cctgccagag
     1081 gatctgattc gcaggatggc ggagcacagc ctccgggtcg tgagcgaacg tgaagacgat
     1141 gctttctggt aacgtcctct tcactggcat gggcgtcact ttataatgac ctttattcgg
     1201 tatttgtatg attgaagcgg tcaattctga caatctgcat gaaattcttc ccctgatcca
     1261 tcgttatcag gaattctatg aagtgtcagc tatcgacgac gacaggaact atcagttttt
     1321 cagccagttt ggtctgcata gcccgatggg ttgtcagttt ctggcgaggg tggagggtgt
     1381 tgctgtcggt tttgccacgg tttatttcac ttactcatcc acggcggtgg ccaaggtggg
     1441 tgtgttgaat gatctctaca cattggcgga tcatcgcgga aaaggcgtgg gccgggcgtt
     1501 gatagagcat tgcctggcgt acgccgtgga aaatggtgcg atcaggctgc aatgggtcac
     1561 cgcaaccgac aatgaagttg cccagcgcct gtatgattca cttgcagtca ataaaaagcc
     1621 gtggctgttc tattcctata aaggctagga gctggcagat cggcgagggt gttggatata
     1681 gtggggccgg ctcgcgacga gccgggcgaa aaattccgta accaatcatg tgagggtggt
     1741 gttttgtgct gttgatcgag accgagatac gcgaatcgga gattcatgga attggactgt
     1801 tttgtgttga gcctgttgca aaggggacca aagtctggga attcaatccg ctgttcgatc
     1861 tgacgctccg cgaggcggag ctgggttccc tgccgccgcc gatgcattcc ttcctgcgca
     1921 aatatgccta ccgttccctg gaaaccggag agctgatcgt caatctcgat ctttcccgcc
     1981 atatgaacca ctccgatgaa cccaacctgg tgtctgatgc gcagtcgaat tatttcgcgg
     2041 tatgtgatct ggcgccgggt accgagctga cctgtgacta ccgcgctttt tcggtggaag
     2101 gctgcagcga ctttctggat gcggttcttg acggagcgca atgaacgatt gtcaggaggc
     2161 ttgatatagt cacggcccag gggctgccaa cggcgacggg aagcgcggtt ggcgggtgaa
     2221 tcaatgatgg ttgggctgat accgggcagt agcatgcata tttcaattca tttgttggcg
     2281 gcattctggg tactcacggc gggtaccctg caacttgtga tgcagaaggg gacactggcc
     2341 caccgggcag tggggtggtc ctggatgctg gccatgctgg tggtggcgg
//
LOCUS       DASUEG010000173         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_699256, whole genome shotgun sequence.
ACCESSION   DASUEG010000173 DASUEG010000000
VERSION     DASUEG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699256"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFX11_03330"
     CDS             <1..221
                     /locus_tag="VFX11_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4937678.1"
                     /translation="APVQNVDIVFCQNLLIYFQRWRKQDIVTKLADRLAPGGMMVLGV
                     GEVIDWHRPDLERVQYEDTLAYVKRNHS"
     gene            240..>3414
                     /locus_tag="VFX11_03335"
     CDS             240..>3414
                     /locus_tag="VFX11_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4937679.1"
                     /translation="MAEHHDYQALEWVKGEIDETLKQAQQSLEAFVDNPDDSTRMRFC
                     LTHLHQVYGTLQMVEFYGAALVAEEMEKLAQALLNQTVPHVADAQEVLMRAILQLPVY
                     LDSIKSARRDMPVVVLPLLNDLRAARGESLLSETALFKPDLAAGSAAAPKEKVSPEAL
                     AQLPELLKKIRQLYQFALVGLIRHQDMPTNLGYMAKSLTKLEQLYSNTPMAQLWWVSA
                     ALVDGVSRNEIELGTSVKMLFGQLDRQLKHSIDTGADRNEPVPMDLLKNLLFYVARCK
                     GDAPRIRKVKEAFKLDSALPSEEMLDVERRRLTGPDRSAISSVVRALLDELGTVKDTL
                     DLFMRGQNSATADLKPVIPTLKQVADTLAVLGIGNPRRVIMEQIDVVGRITATNAMPA
                     DTVLMDVAGALLYVEATLQGMADDAGRNSGAAAASSEPGHVSPEHVVKARAAVIEESR
                     AGLENAKDAISEYHATGFNPAALDLVPDILHSVRGGLLMIPLTYPGALLQSCIDFIRE
                     QLIDKRVKPTWRELDTLADAITSIEYYLERLGDRFPGDEDILAIAEEAVAALGYPAAR
                     VSSEEMAPTLSTPVPAPVTEQPAPVMAEPAAAEVEEEADETAIEAELTLVPGEDEEFI
                     TFSAEELAELEAEPSPAPTAPVVPAASAEPLEEEDNELIDDEVIEIFIEEAGEVLETI
                     NENLPQWAADTENEGALKELRRAYHTQKGSGRMVGAKIIGELAWSVENMLNRVLDHSV
                     PASGAVIRIVETVTGVVPSLVKSFENRTRHTINVDALAAVADALAKGQTPPPIPADLS
                     LAAAAPAPVAPTAAAIAEEAPEAIALAAAEPAQEAAETEAAAEEWGFALEDESGAHAQ
                     ETAAAEAAPAFEQPAVEETEFELADEFASLEDREHVEAAAEMAEESGLEFDLLEPEAE
                     LPILEAETAADWSDLGFVEEQVDDAAAQVPEVAADDFVLAASSDELPLVEPPLVEEIS
                     LAEEIPAAVEAAPAPTAIEIDPVLVDIFSQEAAAHLQTIKEFLAAASEREPLTETLLR
                     ALHTLKGSAHMAGIKPIAELATPIERFI"
BASE COUNT          735 a         1084 c         1054 g          541 t
ORIGIN      
        1 aagcgccggt gcagaacgtt gatatcgtgt tttgccagaa tctgctgatc tattttcagc
       61 gctggcgcaa gcaggacatc gtaaccaagt tggccgaccg gttggcgcca ggcggcatga
      121 tggtgttggg cgtgggcgag gtgatcgact ggcaccgccc ggacctggag agggtccagt
      181 acgaagacac ccttgcctac gtcaagcgca accattcttg agaagagccc ggagcgaaca
      241 tggctgagca ccacgattat caagcgctgg aatgggtgaa gggtgaaatc gatgaaaccc
      301 tgaaacaggc gcagcagtcg ctggaagcgt ttgtggacaa tccggacgat tccacccgga
      361 tgcgcttctg cctcactcat ctgcatcagg tgtacggcac cctgcagatg gtggagttct
      421 acggcgccgc actggtggcc gaagaaatgg agaagctggc ccaggcgctg ctcaaccaga
      481 ccgtgccgca cgtggccgac gcgcaggaag tactgatgcg cgcgatcctg caattgccgg
      541 tgtacctgga cagcatcaaa tccgcccgcc gtgacatgcc ggtggtggtg ttgccgctgc
      601 tgaacgatct gcgtgccgcc cgcggtgaaa gcctgctgtc ggaaaccgcc ctgttcaaac
      661 ccgatctggc cgccggcagt gccgctgcgc caaaggaaaa agtatccccc gaagccctgg
      721 cccaattgcc ggagctgctg aagaaaatcc gccagctgta ccagttcgcc ctggtgggac
      781 tgatccgcca tcaggacatg cccacgaatc tgggctacat ggccaaatcc ctcaccaaac
      841 tggaacaact ctattccaat acgccgatgg cccagttgtg gtgggtgtcg gcggcgctgg
      901 tggacggcgt atcccgcaac gagatcgaac tgggcacgtc agtgaaaatg ctgttcggcc
      961 agctcgaccg gcaactcaaa cacagcatcg ataccggcgc tgaccgcaat gagccggtgc
     1021 cgatggatct gctgaaaaac ctgctgtttt acgtcgcccg ctgcaagggc gatgcgcccc
     1081 gcatccgcaa ggtaaaggaa gccttcaagc tggattccgc gctgccgtca gaagaaatgc
     1141 tcgatgtgga acgtcggcgc ctgaccgggc cggaccgctc cgccatcagc tccgtggtgc
     1201 gtgcgctgct ggatgaactg ggcaccgtga aagacacgct ggatctgttc atgcgggggc
     1261 agaattccgc caccgccgat ctgaaacccg tgattcccac cctgaagcag gtggccgaca
     1321 cgctggcggt gctgggtatc ggcaatccgc gccgggtgat catggagcag attgacgtgg
     1381 tgggccgcat taccgccacc aacgccatgc cggcggacac cgtgctgatg gatgtggccg
     1441 gcgccctgct gtatgtggaa gccacactgc agggtatggc agacgatgcc ggccgcaaca
     1501 gcggcgccgc cgctgccagc agcgagccgg gccatgtttc gccggagcat gttgtcaaag
     1561 cccgcgccgc cgtgatcgag gaatcccgcg ccggcctgga aaacgccaag gacgcgatct
     1621 cggaatatca tgccaccggc ttcaatccgg cggcactgga tctggtgccc gacatcctgc
     1681 acagcgtgcg cggtggcctg ctgatgattc ccctgactta cccgggcgca ctgctgcaga
     1741 gctgcattga tttcatccgc gagcagctga ttgacaagcg cgtcaagccc acctggcgcg
     1801 aactggatac cctggccgac gccatcacca gcatcgaata ctacctggag cgcctgggtg
     1861 accgcttccc cggcgacgaa gacatcctgg ccattgccga agaagccgtg gccgccctcg
     1921 gttatcccgc tgcgcgcgtc tccagcgaag agatggcacc gaccctgagc acacccgtgc
     1981 cagcacccgt gacagaacag ccagcgcctg taatggcgga accggctgct gcagaagtcg
     2041 aagaagaggc agatgaaacg gccatcgaag ccgagctgac cctggtgccc ggcgaagacg
     2101 aggaattcat caccttcagc gcagaagaac tggctgaact ggaagcggaa cccagccctg
     2161 caccgaccgc gccggtggtc ccggccgcca gcgcggagcc gctggaagaa gaagacaacg
     2221 agctgatcga cgatgaagtc attgaaatct tcatcgagga agccggcgaa gtactggaaa
     2281 ccatcaacga aaatctgccc cagtgggccg cggatactga gaacgagggt gccctcaagg
     2341 aactgcgccg tgcctatcac acccagaagg gcagcggccg catggtgggc gcaaaaatca
     2401 tcggcgaact ggcctggtcg gtcgagaaca tgctcaaccg tgtgctggac cacagcgttc
     2461 ccgccagcgg tgccgtgatc cgcatcgtcg agacagttac cggtgtggtg ccgtcactgg
     2521 tgaaatcctt cgagaaccgc accagacaca ccatcaatgt ggatgcactg gccgccgtgg
     2581 ccgatgccct ggccaagggc cagaccccac ccccgattcc cgccgatctc agcctggctg
     2641 ctgccgcacc ggcacctgtc gcaccaacgg ctgctgcaat tgccgaggaa gcgccggaag
     2701 cgatcgccct ggccgcagca gagccggcac aggaagccgc cgaaaccgaa gctgccgccg
     2761 aggagtgggg cttcgcgctg gaggacgaat ccggtgcgca cgcgcaggaa actgccgctg
     2821 ccgaagccgc acctgccttc gagcagcctg ctgttgaaga gactgaattc gaactggcag
     2881 acgaatttgc cagcctggaa gaccgcgagc acgtcgaagc ggcagcggag atggcggaag
     2941 aatccggcct cgaattcgac ctgctggaac cggaagccga attgccgatc ctggaagcgg
     3001 aaaccgctgc ggactggagc gatctcggct tcgtcgaaga acaggtggac gacgctgcag
     3061 cacaggtacc agaggtggcg gccgacgact tcgtcctggc agccagcagc gacgaacttc
     3121 ccctggtgga accgccgctg gtggaagaaa tatccctggc cgaggaaatc cccgccgctg
     3181 tcgaagccgc acccgcgccg accgccatcg aaatcgatcc ggtactggtg gacatcttca
     3241 gtcaggaagc tgccgcccac ctgcagacca tcaaggaatt cctggccgcc gccagcgagc
     3301 gcgagcccct caccgaaacc ctgctgcgcg ccctgcatac cctgaaaggc agtgcccaca
     3361 tggccggcat caagccgatc gccgaactgg ccacgcccat cgagcgcttc atca
//
LOCUS       DASUEG010000174         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_492412, whole genome shotgun sequence.
ACCESSION   DASUEG010000174 DASUEG010000000
VERSION     DASUEG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492412"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFX11_03340"
     CDS             complement(<1..388)
                     /locus_tag="VFX11_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX4937680.1"
                     /translation="MQVAIVGGGQLARMLALAGWPLGLRFAFLVNEGDDTRSVEGLGR
                     VVKCEPGLEPSVLYEALGRPVVITVEKEAVDTGLLEQLNAFCPVHPRPDVLRVCQNRR
                     RQKLWLESLGIAVARHRFAHDAESLRT"
     gene            complement(388..870)
                     /gene="purE"
                     /locus_tag="VFX11_03345"
     CDS             complement(388..870)
                     /gene="purE"
                     /locus_tag="VFX11_03345"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX4937681.1"
                     /translation="MSKPVVSIIMGSRSDWPTLQKATEPLQQLDVPFETQVVSAHRTP
                     DRLFDFARSAPERGIRVIIAGAGGAAHLPGMTAALTWLPVVAVPVMSHTLRGVDSLLS
                     IVQMPRGVAVATQAIGDAGAFNAGLMAAQMLALEDEALRERLIRWRSEQSASVPVEVI
                     "
     gene            complement(898..1836)
                     /locus_tag="VFX11_03350"
     CDS             complement(898..1836)
                     /locus_tag="VFX11_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010558776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HEX4937682.1"
                     /translation="MAMQIDVVIAFFLLGVVARLLRADIHFPVALHQTLTLFLLLAIG
                     LKGGVALAAHASWSLLPQALAVVAFGLVLPLLAFPLLRQIGHFSRVDAASVAAHYGSV
                     SIGTYAVGAALLEARAIPFESYFPFFVALLEVPAIAVGLILARQGQAIAWRPLIGELG
                     RNQGLFLLLGSLLIGVAWAGRVDSVLPLFRDLFHGALALFLLDMGLRAASRLPDLRQH
                     GPFIAAFGVFMPLMGGLLGGLLGWTLGLSTGGVFLLAVLGASASYIAVPAAMQVALPE
                     ANQGLAMTASLGVTFPFNVLAGLPVYALYAHWLTAA"
     gene            complement(1836..2030)
                     /locus_tag="VFX11_03355"
     CDS             complement(1836..2030)
                     /locus_tag="VFX11_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937683.1"
                     /translation="MHKRQIQFGWSVGLGLALAGAGLALVDGAGWGLLYAGLLVAGLT
                     AAAQKRPLAATIPLERHRRD"
     gene            2153..>2570
                     /locus_tag="VFX11_03360"
     CDS             2153..>2570
                     /locus_tag="VFX11_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019441305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4937684.1"
                     /translation="MASRLHAHIGTLRQLEILLAVYDQGSVTAASAHLHLTQPTVSMQ
                     LKKLAEAIGMPLYEQIGRRLVFTDAGRALVSTAREVLDQFARLDMTLSELRGLKAGHL
                     RLAVVTTSKYFIPHLLGPFCRRYPAIDVQMKVANREQ"
BASE COUNT          563 a          886 c          742 g          379 t
ORIGIN      
        1 cagtccgcag cgattcagca tcatgggcaa agcggtgacg ggcgactgca atgcccagag
       61 attccagcca gagcttctga cggcgccggt tctggcagac acgcagcaca tccggtcgtg
      121 gatgcacggg acagaaggca ttcagttgtt ccagcagacc cgtgtccacc gcctcctttt
      181 ccaccgtgat gacaaccggc cgacccaggg cctcgtacag caccgacggt tccaggccag
      241 gttcgcactt caccacccgg cccagtccct ccacgctgcg cgtgtcatcc ccctcattca
      301 cgagaaaagc aaaccgcagt ccgagcggcc agccggcaag cgccagcatg cgcgccagtt
      361 ggccccctcc cacaatcgcg acctgcatca gatcacctcc acgggcacgg acgccgattg
      421 ctcgctgcgc cagcggatca ggcgttcgcg cagcgcttcg tcttccagtg ccagcatctg
      481 ggccgccatc agaccggcgt tgaatgcccc ggcatcacca atggcctggg tggcgactgc
      541 cacgccccgg ggcatctgca caatcgacaa cagactgtcg acacctcgca gcgtgtggct
      601 catcaccggc actgccacca ccggcagcca ggtgagcgcg gccgtcatgc ccggcagatg
      661 ggccgcaccg cccgcccccg caatgatcac gcgaatgccg cgctccggcg cgctgcgggc
      721 aaagtcgaac aggcggtccg gcgtgcgatg ggccgagacc acctgcgtct cgaacggcac
      781 gtccagctgc tgcaacggtt ccgtggcttt ctgcagggta ggccagtcgg atcgtgatcc
      841 catgatgatg ctgacaacag gttttgacat aggcaggctc cagaaataaa tgagacgtca
      901 ggcagcggta agccagtgtg cgtagagcgc atacacaggc aaccctgcca atacattgaa
      961 gggaaaagtg actccgaggg acgctgtcat tgccagcccc tggttggctt cgggcaatgc
     1021 cacctgcatg gccgccggca cggctatgta ggaagcgctg gcacccagca cggccagcag
     1081 gaacacgccg ccggtggaca agcccagggt ccagcccagc agacctccca gcaaaccgcc
     1141 catcaaaggc atgaacacac cgaaggcggc gatgaacggc ccgtgctggc gcaggtccgg
     1201 caaacggctg gcggcgcgca atcccatatc cagcaggaac agcgccagtg ccccatggaa
     1261 caggtcgcgg aacagcggca gcacagagtc caccctgccg gcccatgcca ctccgatcag
     1321 cagtgaaccc agcagcagga acagtccctg gttgcggccg agctcaccga tcaatggccg
     1381 ccaggcaatg gcctggccct gccgcgccag gatcagaccc accgcaatgg cgggcacttc
     1441 cagcagggca acaaagaacg ggaaataaga ttcaaacggg atggcccggg cttccagcaa
     1501 tgcagccccc acggcgtagg tgcctatgct gaccgaaccg taatgagcag ccacggatgc
     1561 tgcatcgact cgcgagaaat ggccaatctg gcgcaacagg ggaaaggcca gtaacggcag
     1621 caccagcccg aaggccacca cggccagcgc ctgcggcaac agcgaccagc tggcatgcgc
     1681 cgccagcgcc acaccgccct tcaggccaat ggccagcagc aggaacagcg tgagcgtctg
     1741 gtgcagcgcc accgggaagt ggatatcggc ccgcagcagc cgcgccacca cacccagcag
     1801 gaagaaggcg atcaccacat cgatctgcat cgccatcagt ccctgcggtg ccgctcaagc
     1861 ggaatcgtcg ccgccagcgg gcgtttctgc gccgccgcgg tgaggccagc caccagcaac
     1921 ccggcgtaca gcaggcccca gccggcccca tctaccaacg caagcccggc gcccgccagg
     1981 gccagtccca atcccacact ccagccaaac tggatctgtc gcttgtgcat cgtctgatct
     2041 ccctgcaata gaaggaacac ccttctcttt caggagcgca gcatgcgcca tccacaaatt
     2101 agataaaaat agatattatt taagcatcac atagataaag atctaattcg tcatggccag
     2161 ccgtctacat gctcacattg gtaccctgcg ccagctggaa atcctgcttg ccgtctacga
     2221 tcagggcagc gttaccgcgg cgtcagccca tctgcacctg acacagccca ccgtctcgat
     2281 gcagctgaaa aagctggcgg aggccatcgg catgcccctg tacgagcaga tcggcagaag
     2341 gctggttttc accgatgccg gccgcgcact ggtgagcacc gcccgagagg tactggacca
     2401 gtttgcgcgc ctggacatga ccctgtcgga attgcggggg ctgaaggccg gtcacctgcg
     2461 actggccgtg gttaccacgt cgaaatattt cattccgcac ctgctggggc cattctgccg
     2521 ccgctaccct gccatcgacg tgcagatgaa agtggccaac cgcgaacaga
//
LOCUS       DASUEG010000175         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1675256, whole genome shotgun sequence.
ACCESSION   DASUEG010000175 DASUEG010000000
VERSION     DASUEG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675256"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VFX11_03365"
     CDS             complement(<1..1393)
                     /locus_tag="VFX11_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4937685.1"
                     /translation="MDQPAFQSLLRSDAFPHPVSALQVRETHISRVLLTGDFVYKIKK
                     PVNFGFLDFSTLERRRHFCHEEVRLNRRTCPNIYLDVVAVVAREGQLFLYGDGDIVEY
                     AVKMRQFDADATLADWFASGKNVTPESLQALGYRLAEFHAGAAIASADTAWGTADAVL
                     APVLENFRQIEPLVKDEHQRAQLGEIKRWSLEQAQVLRELFTARRQQGFVRELHGDLH
                     AGNIAWIDQQWLPFDCIEFNPDLRWIDTASDLAFLLMDLEFSGLRAQANRILNAYLEF
                     SADYGLLAVLDFYRIYRAMVRAKVSILRRQQAGLGLEQAAVLEQAFAGYLDYCIVTRP
                     SRPLFLAITTGISGSGKSTVARLVAQQSGAIQLRSDVTRKRLCGLKPLQPSSQVPGGI
                     YTEAISRRTFDQLVQDAGAVLAAGFPVIVDATFLKRAHRQPFLALAQQRGVQAAILVC
                     AADHGELVRRIQAR"
     gene            complement(1424..2332)
                     /locus_tag="VFX11_03370"
     CDS             complement(1424..2332)
                     /locus_tag="VFX11_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4937686.1"
                     /translation="MTTEAELAHQNHARLLHLAATASVLVAGLLMLIKAYAWWVSDSI
                     SLLASLTDSALDALASLLNFIAVRYSLQPADAEHRFGHGKAESLAGLGQALFIMGSAV
                     FLLVRAVDRMLNPQPLEQLDVAVAVMLVSIVLTFALVLYQRHVITLTHSPAIKADSLH
                     YLSDLLTNAGIIAALLLSWAGWPQVDPVLAIAIALYIFYTAVQIWNESIQLLLDRELP
                     EEELRRIEAIALQREEVLGVHGLRTRQSGHVKIVQMHVELDGNMPLARAHRICDQVES
                     DILREFPNADVLIHQDPHMPGATHRN"
     gene            2443..3234
                     /gene="amrB"
                     /locus_tag="VFX11_03375"
     CDS             2443..3234
                     /gene="amrB"
                     /locus_tag="VFX11_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX4937687.1"
                     /translation="MSDIREPAVAGLFYPEKPQELQQVINGFLETAPHSHLDRPKAIV
                     VPHAGYVYSGSMAARAYVTLEHHAREIRRVVLLGPSHRVGFRGIAFCSADSYKTPLGL
                     IPVEQSAFVAISNLPNVGMLDRAHAQEHSLEVQLPFLQSVLRDFTLVPLVVGDVEPED
                     VTRVLEKLWGGPETLIVISSDLSHYHDYATARELDAETCHAVESCRPDQIGYEQACGR
                     IPLNGLLQLAQRKHLKVTTLGLCNSGDTAGDRQRVVGYGAWAFSE"
     gene            3231..3785
                     /gene="amrA"
                     /locus_tag="VFX11_03380"
     CDS             3231..3785
                     /gene="amrA"
                     /locus_tag="VFX11_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX4937688.1"
                     /translation="MTALSATQRQRLLEIARESIRHGLSHGKPLSVRDEDAALLQPGA
                     SFVTLNLHDELRGCIGSLEAHQPLVKDVAQNAFSAAFRDPRFPPLTDREFAALDLHIS
                     ILSPSEPLHFSSESDLLRQLRPGIDGLVLSDGYHRGTFLPQVWEQLPEPAEFLAHLKR
                     KAGLPVNYWSPTLQVERYTVDSIP"
     gene            3840..>4659
                     /locus_tag="VFX11_03385"
     CDS             3840..>4659
                     /locus_tag="VFX11_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4937689.1"
                     /translation="MSNDLKLGAFYLLLGEFLLAMMAALIKHLSGDVPQEVLVFSRNL
                     FGLVALLPIVLHQGVRNLKTRRLGVHLQRSIVGLIAMYGYFYVIAHLPLAEAVLVKLS
                     APFFLPIIAFFWLKEKIRLRTALAILLGFFGVVLVLRPGSDTFQPVALVGIGAAVLAS
                     LAKVTIRDMADTEPTYRVVFYFGLLATLVSAVPLLWGWQMPPAHTWIWLFVIGVAGTG
                     GQMLMTKAYQIANPGQVGPYTYASVIYAAILGWLFWDEALLITTLIGSVLIVASG"
BASE COUNT          939 a         1433 c         1393 g          894 t
ORIGIN      
        1 ggcgcgcctg gatgcgccgt accagttcac catgatccgc cgcgcaaacc agaatcgctg
       61 cctgcacccc ccgctgctgc gccagggcaa gaaacggctg ccggtgggca cgcttgagaa
      121 aggtggcgtc cacaatgacc ggaaaaccgg ctgccaggac tgcgccggcg tcctgtacca
      181 gctgatcgaa tgtgcgacgc gaaatcgcct ctgtgtaaat tccgccaggc acctgcgagc
      241 tcggctgcag gggtttcagg ccgcatagac gtttgcgagt gacatcagaa cgcaactgga
      301 tcgccccgct ctgctgcgcc accaggcgcg cgacggtgct tttaccgctg ccggaaatgc
      361 cggtagtgat cgcgaggaac agcggacgcg acggccgtgt gacgatgcag taatccaggt
      421 aaccggcaaa tgcctgctcg agtactgcgg cctgctccag gccgagacca gcctgctggc
      481 gacgcagaat gctcaccttg gcccgcacca tggcgcggta tatccgatag aaatccagca
      541 ccgccagcaa gccataatcg gcactgaatt ccagataggc attcaggatg cggttggcct
      601 gggcacgcaa gccgctgaat tccagatcca tcagcagaaa tgccagatcg ctggcggtat
      661 cgatccagcg caggtccgga ttgaattcga tgcagtcgaa cggcaaccac tgctggtcga
      721 tccaggcaat attgccggcg tgcagatcgc cgtgcagctc acgcacgaaa ccctgctgcc
      781 ggcgcgcggt aaaaagttcg cgcaacacct gcgcctgctc cagcgaccag cgttttattt
      841 cgcccagctg cgcgcgctgg tgttcgtcct tcaccagtgg ttcaatctgg cggaaatttt
      901 ccagcaccgg cgccagcacg gcatcagcgg tgccccacgc tgtatccgcc gaggcaatgg
      961 ccgcgccggc gtgaaactcc gccagccggt aacccagtgc ctgcagggat tccggtgtca
     1021 catttttccc gctggcaaac cagtccgcca gcgtggcatc cgcatcgaac tggcgcattt
     1081 ttacggcgta ctccacgata tcgccgtcac cgtacagaaa cagttgaccc tcgcgggcca
     1141 cgaccgctac cacgtccaga taaatgttcg ggcaggtgcg acggttgagg cggacttcct
     1201 cgtggcaaaa atgccggcgc ctctccagcg tggaaaaatc cagaaatccg aaattcaccg
     1261 gcttcttgat tttgtagacg aagtcgcccg tgagcagcac gcgtgaaata tgggtttcac
     1321 gtacctgcag cgcagatacg ggatgcggga acgcgtcgct gcgcagcaaa gattggaaag
     1381 cgggctggtc catggcatgt cgtccgtgag caccgaagtg cattcagttg cggtgagtag
     1441 cgccaggcat gtgggggtcc tggtgaatca gcacatcggc attgggaaat tcccgcagga
     1501 tgtctgactc cacctgatcg cagatgcggt gcgcgcgggc cagcggcatg ttgccatcca
     1561 gttctacatg catctgtacg attttcacat ggccggactg gcgggtgcgc aaaccatgca
     1621 cacccagcac ctcttcacgc tgcagcgcga tggcttcgat tcgacgcagc tcctcttcgg
     1681 gcaactcccg atccagcagc aactggatcg attcattcca gatctgcacg gcggtgtaaa
     1741 aaatatagag cgcaatggca atggccagca cgggatccac ctggggccag ccggcccacg
     1801 acagcagcag cgcggcgatg ataccggcgt tggtcagcag atccgacagg taatgcagtg
     1861 aatccgcctt gatggcgggg gaatgggtca gcgtgatcac gtgacgctga tacagcacca
     1921 gggcaaaggt gagcacaatc gacaccagca tgaccgccac tgccacatcc agctgctcca
     1981 gcggttgcgg attcaacatg cggtccaccg cccgcaccag cagaaaaaca gcggagccca
     2041 tgatgaacag cgcctggccc agaccggcca gcgactccgc cttgccgtga ccgaaacgat
     2101 gttcggcgtc ggccggttgc agcgagtagc gcacagcaat gaaattgagc agcgatgcaa
     2161 gcgcgtcgag ggcggaatcg gtcagcgacg ccagcaggct gatggaatcg gaaacccacc
     2221 aggcataagc cttgatgagc atgagcaggc ccgcaaccag cacagaggcg gtggcagcca
     2281 gatgcagcag gcgtgcatga ttttggtggg caagttcggc ttcagtcgtc ataacctgcc
     2341 attttaacag catgtcatca caacaaaacc cgcaagacga ttgaatttga caatcgcgcc
     2401 cccattttta acgcaaggca aatgaaaacg aggtgcattc ccgtgagcga catccgcgag
     2461 ccagcagtag ctggtctgtt ctatccggaa aaaccgcagg aattacaaca ggtcatcaac
     2521 ggctttctgg agacggctcc ccattcccat ctggaccggc ccaaagcgat cgtggtgccc
     2581 catgccggct atgtgtattc cggcagcatg gccgcgcgcg cttatgtgac gctggaacat
     2641 catgcccgtg aaatccggcg ggtggtgttg ctggggcctt cgcaccgggt cggctttcgc
     2701 ggcatcgcat tttgcagcgc cgattcctac aagacaccgc tgggcctgat cccggtggaa
     2761 cagtccgcct tcgtcgccat ttccaacctg cccaatgtcg gcatgctcga ccgcgcccac
     2821 gcccaggaac acagcctgga agtgcagctg ccctttctgc aatccgtgct gcgggatttc
     2881 accctggtac cgctggtagt gggcgatgtg gagccggaag atgtcaccag ggtgctggaa
     2941 aagctctggg gcggacccga gacgctgatc gtgatcagtt ccgatctgag ccactatcat
     3001 gactacgcaa ccgctcgcga actcgacgcg gaaacctgcc acgcggtgga aagctgccgc
     3061 cccgaccaga tcgggtacga gcaggcctgc ggtcgcattc cgctgaacgg actgctgcaa
     3121 ctggcacaac gcaaacatct gaaagtcacc acactgggcc tgtgcaactc tggcgacacg
     3181 gccggtgacc gtcagcgcgt ggtgggttat ggcgcctggg cattttccga atgaccgcgc
     3241 tctcggccac acagcgccag cgcctgctgg aaattgcgcg ggaatccatc cgtcatggcc
     3301 tttcacatgg caaaccgtta tccgtgcggg atgaggatgc tgcgctgctg cagcccggtg
     3361 ccagtttcgt cacgctgaac ctgcacgacg agttgcgcgg ttgcatcggt tcactggagg
     3421 cccatcaacc gctggtgaag gatgtggcgc agaatgcctt cagtgctgcg tttcgcgatc
     3481 cccgctttcc acccctgacc gaccgcgaat ttgctgccct ggatctgcat atatccattc
     3541 tttcccccag tgagccgctg cacttttcca gtgaaagcga tctgctaaga cagctgcgcc
     3601 cgggcatcga cggtctggtg ctgagtgacg gctatcatcg cggcaccttc ctgccgcaag
     3661 tgtgggaaca attgccggaa ccggcggaat ttctcgccca cctcaagcgc aaggccggtt
     3721 tgccggtaaa ttactggagc ccgacattgc aggtggaacg ttacaccgtc gacagcattc
     3781 cctgaccgca ccaggcccgg ggccgcctgc tatactgcgc gctgcttcgg agacgcagta
     3841 tgtccaacga cctgaaactg ggcgcattct atttgctgct gggggaattc ctgctggcca
     3901 tgatggcggc gctgatcaag cacctgtccg gcgatgtgcc gcaggaggtg ctggtattca
     3961 gccgcaacct gttcggtctg gtggcgctgt tgccgatcgt gctgcaccag ggcgtgcgca
     4021 atctgaaaac ccggcggctg ggtgtgcatc tgcaacgctc catcgtcggg ctgatcgcca
     4081 tgtacggcta tttctatgtg atcgcccacc taccgctggc agaggcggtg ctcgtcaaac
     4141 tgtcagcgcc gtttttcctg cccatcattg cgttcttctg gctgaaagaa aaaatccgtc
     4201 tgcgcacggc gctggcgatc ctgctgggat tcttcggcgt ggtattggtg ttgcggcccg
     4261 ggtccgacac gtttcagcca gtggcgctgg tgggcatcgg cgcggcggtg ctggccagcc
     4321 tggccaaggt gacgatccgc gacatggccg ataccgagcc cacctatcgc gtagtgttct
     4381 atttcggcct gctggcaacg ctggtgtcgg cagtgccgct gctatggggt tggcagatgc
     4441 caccagccca cacctggatc tggctgttcg tgatcggtgt ggccggcacc ggcgggcaga
     4501 tgctgatgac caaggcctac cagatcgcaa accccggcca ggtgggcccg tacacctatg
     4561 cctcggtaat ttacgccgcc atcctgggct ggctgttctg ggatgaagcc ttactcatca
     4621 cgacgctgat cggcagcgtg ctgattgtgg cgtccggcg
//
LOCUS       DASUEG010000176         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_357946, whole genome shotgun sequence.
ACCESSION   DASUEG010000176 DASUEG010000000
VERSION     DASUEG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357946"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFX11_03390"
     CDS             <1..958
                     /locus_tag="VFX11_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008224552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4937690.1"
                     /translation="RPLTLDDADALTRLPHVDYVVPSVQGAGTVEAGERSRNSDIIGA
                     NHALNDAWQFKLAMGSSLPKDESGRSRNFAVLGSKLKEELFGARNPLGQFVRVGGIRF
                     RVVGVMEEKGNMLGIDLDDIVFIPVDKALTLFNREGLMQIDVVFAESTTSTIMAQAVR
                     KLMLERHGNEDFTIVTQDQMLKSLDKILSVLTAAVGALGAISLLVGAVGILTIMTTTV
                     RERTAEIGLLNALGTSQQQILLLFLWEAVALAFAGGLAGIVTMVGLILLIQWLAPTLP
                     LTLSPFYLGLSLALSVIIGLVAGLAPARTAARMNPIDALRTE"
     gene            1007..1975
                     /locus_tag="VFX11_03395"
     CDS             1007..1975
                     /locus_tag="VFX11_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937691.1"
                     /translation="MGQVETHLSATPKTGDTPQNAQQQASLAIDLFHAHTLRMARHEL
                     LITILVLSRFIQRHRGATLALLGGDKSFRPQVQTLQQQINAQLEFIHCLNTTAHESIA
                     ENDWKQLSLGWHTIIKDLENDAIHHSFEFHSHLLELLLRVARQLSEAVLYQPPLAQMP
                     EALRNRVEKTCLEPLQNLNQTCVIELYELVEYLARIRGMGTHMAAVGHSDTDSDTRVA
                     FWLQEFRYRKDRFDQNLQVLPSAYLPFIPALRALPNLNMKLNYFINLLGHEMTSQRSI
                     KIPSHKLFLMGTEIIDGYLNVMDQANAVIREQLHQMNMMCLQRLME"
     gene            2274..>2717
                     /locus_tag="VFX11_03400"
     CDS             2274..>2717
                     /locus_tag="VFX11_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937692.1"
                     /translation="MTAETSDNLSAGINLRELASSAHFALKLFFLLLTAAFSFMLDLQ
                     TLGLACVFLGSASLLIERRLSVRDPASTILLLSGLALCLLQWLPTVQSALPANFLPAL
                     PFVAVFTFYLGYSLLRQPFAELFAQISSLPALRRQHTQLWLIQSAA"
BASE COUNT          528 a          858 c          772 g          559 t
ORIGIN      
        1 ccggccactc actctggatg atgccgatgc gctgacgcgg ctgccccatg tggactacgt
       61 ggtgcccagt gtgcagggtg caggcaccgt ggaagccggc gaacgctcgc gcaacagcga
      121 catcatcggc gccaaccacg ccctcaacga cgcctggcag ttcaagttgg ccatgggcag
      181 ctcgctgcca aaagacgaat ccggccgcag ccgcaatttc gcggtgttag ggtcgaagct
      241 gaaagaggaa ctgttcggcg cacgcaatcc actgggccag tttgtccgcg tcggcggcat
      301 ccgcttccgg gtggtggggg tgatggagga aaagggcaat atgctgggca tcgacctgga
      361 cgacatcgtg ttcatcccgg tggacaaggc gctgaccctg ttcaatcgcg aagggctgat
      421 gcagatcgac gtggtgttcg ccgaaagcac gacgtccacc atcatggcgc aggccgtgcg
      481 caagctgatg ctggaacgtc acggcaacga ggacttcacc atcgtcaccc aggaccagat
      541 gctgaaaagc ctggacaaga tcctgtcggt actcaccgcc gccgtgggag cgctgggggc
      601 gatctcgctg ctggtggggg ccgtgggcat actcaccatc atgaccacca cggtgcggga
      661 acggacggcg gaaatcggcc tgctcaatgc cctgggcacc tcgcagcagc agattctgct
      721 gctgttcctg tgggaagcgg tggcgctggc ctttgccggc gggctggccg gcatcgtcac
      781 catggtgggc ctgatcctgc tgatccagtg gctggcgccg acgctgccgc tgaccctgag
      841 ccccttctac ctgggtctgt cgctggcgct gtccgtcatc atcggcctgg tggctgggct
      901 ggcgcctgcc cgcaccgctg cccgcatgaa ccccattgac gcactgcgca cggaatgatg
      961 gcacggggtt tgaattaccc ctgcaaaggt ccttctggag attttcatgg gtcaggtcga
     1021 aacacacttg tccgccaccc ctaaaaccgg cgacacccca caaaacgcac aacaacaggc
     1081 ttccctggcc atcgatttgt ttcacgcgca cactctgcgc atggcccggc acgaactgtt
     1141 gatcaccatt ttggttctca gccgcttcat tcagcgccat cgcggtgcaa cgctggccct
     1201 gctggggggc gacaagagct tccggcccca ggtgcagacc ctgcagcaac agatcaatgc
     1261 ccagctggaa ttcatccact gcctgaatac caccgcccac gaatccatcg ccgaaaatga
     1321 ctggaagcag ctgagcctgg gctggcacac catcatcaag gatctggaaa acgacgctat
     1381 ccaccacagt ttcgagttcc attcccacct gctggaactg ctgctgcggg tggcacgcca
     1441 gctgagcgag gccgttctgt atcagccgcc gctggcacag atgcccgagg ccctgcgtaa
     1501 ccgggtggag aagacctgcc tggagccact gcagaacctg aaccagacgt gcgtgatcga
     1561 gctgtatgaa ctggtggaat acctggcgcg gattcgcggc atgggcaccc acatggccgc
     1621 cgtgggccac agcgacaccg attccgatac ccgggtggcg ttctggctgc aggaatttcg
     1681 ctaccgcaag gatcgcttcg accagaacct gcaggtgctg cccagcgcct acctgccgtt
     1741 cattcccgcc ctgcgcgcgc tgcccaacct gaacatgaaa ttgaattact tcatcaatct
     1801 gctggggcac gagatgacgt cgcagcgttc gatcaagatt cccagccaca aactgttcct
     1861 gatgggcacc gaaatcatcg acggctacct gaacgtgatg gatcaggcca atgccgtgat
     1921 tcgcgagcag ctgcatcaga tgaacatgat gtgcctgcag cgcctgatgg agtaatccgg
     1981 aattccactg tgcagccacc cggttgcaca gtggccacaa aatgcagtct ccccggaaat
     2041 tctgggcgtt aacatgcacg acactgatgc cagcgttgtc aaacctgttt cctgctctcc
     2101 ccacccccga aacaatgact gccgcgccac cgtcaatccc ggtagcactt gaaatattcc
     2161 gtgtgatagc atctccgaac ttttttaggc tcggtgaata ttgatgtggg cctgctctat
     2221 gcccgcatcg accttgcatc gacaactccg acaaccgact aggcgataag ggaatgactg
     2281 ctgaaacgtc tgacaatctg agcgctggca tcaatctgcg cgaactggct tcttccgctc
     2341 actttgccct gaaactgttt tttctgctgc tgacggctgc tttcagtttc atgctggatc
     2401 tgcaaacgct ggggctggcc tgcgtttttc tgggcagcgc atcgctgctg attgaacgcc
     2461 gtctgtcagt gcgcgatccg gcttccacca tcctgctgct gagtggcctg gctctgtgcc
     2521 tgttgcagtg gcttcccacc gtgcaatccg cgctgcccgc gaattttctg ccggcactgc
     2581 ccttcgtggc cgtattcact ttctatctgg gttattcact gctgcggcag ccattcgccg
     2641 aactgtttgc ccagatttcc tcattgccag ccttgcgccg tcagcacacg caactgtggc
     2701 tgatccagtc cgcggct
//
LOCUS       DASUEG010000177         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_890096, whole genome shotgun sequence.
ACCESSION   DASUEG010000177 DASUEG010000000
VERSION     DASUEG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890096"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2052)
                     /locus_tag="VFX11_03405"
     CDS             complement(<1..2052)
                     /locus_tag="VFX11_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4937693.1"
                     /translation="MTVFELISRLGAAGIKLWVEEGQLKFKAPKGALTPELKDALVAR
                     KQDVIDFISGTRISGGDDADRIPAVDRSKPLPLSHAQNRLWFIEQLAPGNSTFHIPAP
                     LFLTGILDQSALEKAFLKLLQRHEALRTVFRVVDDEPVQIVQQVERFVVPMDDLTTLP
                     SHERDAEARRRIEREIRTPFNLEQGPLLRAHLYKLDDNRYGLLVVMHHIVTDGWSMTI
                     FVREIAALYAAERMGMNSPLPPLDIQYGDFAQWQRHWLQGEMLERQLGYWRTQLAGAP
                     EQLMLPFDRPRPPMQTSNGAVLDVRLDDALTAQIKKVARQFDTTPYVILLAAYKIVLA
                     RWARQKDLCVGMPVAGRTRPEVEGLIGFFVNTLVIRTRQDGKPSFGQFVAQVKEQVLG
                     AQSHQDVPFEAIVDDLNIPRNLSFAPVYQVALSLTAAEGSSQKSVLGGLEIEPMPVEL
                     VAARLDLTLMLADYGDHLGGMLEFNTDLFDHATVGTFIEHFRRVLDGATRDVDQCIDS
                     LQLVNDVELRRLLVLDDGAEAVLPLAPMQRDFCLDSVREPDTQRNSIGAWLALPFAVD
                     AEQWRQALQAVVAANPFLRARFVESSRTWLEPFYQVIARNAPAQLELLDWRDRALPEA
                     ELQQALESLALPAWTVLGGPLTRHYLVRVSDQKHIAVVASHHAVFDGVSKRQYFHQML
                     DA"
     gene            complement(2049..>3077)
                     /locus_tag="VFX11_03410"
     CDS             complement(2049..>3077)
                     /locus_tag="VFX11_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX4937694.1"
                     /translation="GGEACDPLQVRAVLNHPSRPKHLLHMYGPAENTTYATWYEVRDV
                     LPGATTVPVGYPVSNSRLYLLDTQLRPVPIGVPGEIFVAGDGLAHGYLNRPELTRDVF
                     LPDPFDDGKLMYKTGDLGRMQPDGAVEILGRIDDQVKIRGFRIELGEVENAVNQLPGV
                     RESVVAVRLDANGNKQLVAYCKRPQGGMTLAVADLKARLKQHLPDFMVPSAIVMLDEL
                     PITPNGKVDKRALPAPAPEDYASNAYVAPRNAIEDTLVNIWRDVLGVDQIGVYDDFFE
                     LGGHSLLVTKVATRLKQQLGVELPLRTLFEVPTVAALAEIFSAVSATSSVTDELAAGD
                     EEFEEGSL"
BASE COUNT          618 a         1005 c          881 g          573 t
ORIGIN      
        1 ggcatccagc atttgatgga aatactggcg cttgctgacg ccgtcaaaca ccgcgtgatg
       61 ggatgccacc actgcaatat gtttctgatc actcacgcgc accagataat gccgcgtcaa
      121 cggcccgccc agcacagtcc aggccggcaa tgccagagat tccagcgcct gctgcaattc
      181 tgcttccggc aaggcgcggt cgcgccagtc cagcaactcc agttgtgccg gcgcattgcg
      241 cgcaatcacc tgatagaagg gttccagcca ggtgcgactg ctttccacaa accgcgcccg
      301 cagaaacgga ttggcggcga caacggcctg caatgcctga cgccattgct ccgcgtccac
      361 cgcaaacggc agcgccagcc aggcaccaat gctgttgcgc tgggtatcgg gttcgcgcac
      421 gctgtccagg cagaaatccc gttgcatggg tgccaacggc agcacggctt cggcgccatc
      481 atccagcacc agcagacggc gcagttccac atcgttcacc agctgcagac tgtcgatgca
      541 ctgatcgacg tcgcgggttg cgccatccag cacgcgacgg aaatgttcga tgaacgtgcc
      601 aacagtagcg tgatcgaaca gatcggtatt gaattccagc atgccaccga gatgatcgcc
      661 gtaatccgcc agcatcaggg tgagatcgag acgggccgcc accagttcca ccggcatcgg
      721 ctcgatttcc agaccgccca gcacggattt ctgtgacgaa ccttctgccg cagtgagcga
      781 cagcgccacc tgatacaccg gcgcgaaact caggttgcgc ggaatattga gatcgtcaac
      841 gatggcttcg aacggcacgt cctggtgact ctgcgcaccc agcacctgct ccttcacctg
      901 ggccacgaac tggccgaagg aaggcttgcc gtcctgccgc gtacggatca ccagcgtgtt
      961 gacgaaaaag ccgatcagcc cttccacttc cggccgggta cggcccgcca ccggcatgcc
     1021 tacacacaga tccttctgcc gcgcccagcg tgccagtacg atcttgtagg cggccagcag
     1081 gatcacatag ggcgtggtat caaattgacg cgccactttt ttgatttgtg cggtgagcgc
     1141 gtcgtcaaga cgaacatcca gcaccgcgcc attggatgtc tgcatcggcg gacgcggacg
     1201 gtcgaatggc agcatcaact gctccggtgc accggccagc tgcgtgcgcc agtaacccag
     1261 ctgccgctcc agcatttcac cctgcaacca gtgtcgctgc cactgggcaa aatcaccgta
     1321 ttgaatgtcc agcggcggca gcggcgaatt catgcccatc cgctccgccg catacagcgc
     1381 ggcaatttcc cgcacgaaaa tcgtcatcga ccagccgtcg gtcacgatgt ggtgcatcac
     1441 caccagcagg ccgtagcggt tgtcatccag tttgtacaga tgggcgcgca gcagcgggcc
     1501 ctgctccaga ttaaaaggcg tgcggatttc ccgctcgatg cgacgacggg cttccgcgtc
     1561 acgctcatgg ctgggcagcg tggtgagatc gtccatcggc accacgaacc gttctacctg
     1621 ctgcacgatc tgcaccggct cgtcatcaac cacgcggaat acggtgcgca gtgcttcatg
     1681 gcgctgcaac agtttcagaa acgccttttc cagcgcggat tgatccagaa tgccggtgag
     1741 aaacaggggg gcgggaatat gaaaggtgct gttgcccggc gccagctgct cgatgaacca
     1801 gagccggttc tgggcgtggg acaacggcag cggcttgctg cggtctaccg ccggaatgcg
     1861 atcggcatcg tcgccaccac tgatgcgggt gccgctgatg aaatcgatga catcctgctt
     1921 acgagccacc agcgcgtctt tcagttccgg cgtcagtgcg cccttgggcg ccttgaattt
     1981 cagctgccct tcttccaccc acagcttgat gccggctgcg ccgagccggg aaatcaattc
     2041 aaacactgtc ataacgaacc ttcctcgaac tcctcatccc cggccgcaag ctcatccgtc
     2101 acggaactgg tggcgctgac ggcgctgaaa atttcggcca gcgcggccac cgtgggtact
     2161 tcaaataatg tgcgcagggg caattccact cccagctgct gcttcaaccg cgtggcaacc
     2221 ttggtcacca gcagcgaatg gccgccgagt tcgaagaaat cgtcgtacac gccgatctgg
     2281 tccacaccca gcacgtcgcg ccagatattc accagggtgt cttcaatcgc gttgcgcggt
     2341 gccacatagg cattcgacgc gtaatcctcc ggcgcgggcg cgggcaatgc gcgcttgtcc
     2401 accttgccgt tgggcgtgat cggcagctcg tccagcatga cgatggcact gggcaccatg
     2461 aaatccggca aatgctgctt gaggcgcgcc ttgagatccg ccaccgccag cgtcatgccg
     2521 ccctgcggcc gtttgcagta agccaccagc tgtttgttgc cgttcgcatc gagacgcacg
     2581 gccaccacgg attcgcggac gcccggcaac tggttgacgg cgttttccac ttcgcccagc
     2641 tcgatacgga aaccacgaat ttttacctga tcgtcgatac ggccaagaat ttccactgcg
     2701 ccatccggct gcatgcggcc gaggtcaccg gtcttgtaca tcagcttgcc gtcgtcgaac
     2761 ggatcgggca ggaacacatc gcgggtgagc tcgggccggt tcaggtagcc gtgggccaga
     2821 ccatcccccg ccacgaaaat ttcgccgggc acgccaatcg gaaccggccg cagttgcgta
     2881 tccagcagat acagccggct gttcgacacc ggataaccca ccggcaccgt ggtggcaccc
     2941 ggcagcacat cacgtacttc ataccaggtg gcataggtgg tgttttccgc cgggccatac
     3001 atgtgcaaca gatgtttggg ccggctggga tgattcagca ctgcgcgcac ctgcagcgga
     3061 tcgcaggcct cgccgcc
//
LOCUS       DASUEG010000178         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_559876, whole genome shotgun sequence.
ACCESSION   DASUEG010000178 DASUEG010000000
VERSION     DASUEG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559876"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4196)
                     /locus_tag="VFX11_03415"
     CDS             complement(<1..>4196)
                     /locus_tag="VFX11_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937695.1"
                     /translation="QIIQHCTQPDVFGYTPADFPLAKLDQQQIDRLVGKQRGIVSVYP
                     LSPMQEGMLFHSLFDNDAGVYFEQLSVEVIGSVDRDLLKHAWQFVVNRHPALRSAFVW
                     QDLDRPLQIVHNRIDMEVNEFDWRNTDPVQEQHRLNVWLEKDRQRGFDFDKPGLMRLA
                     WLDLPGHRSRLIWSFHHIVLDGWSLPLVMGEVFQTYGALAKGVQPQFHQPGSYEDFIA
                     YLERADKGDALQFWKLYLAGFEAATPLPNKKPRSNSHDKVFDEAHLTLDETLTSRLQN
                     FAREQHVTLNTVIQAAWGVLLARYSGEKDVVFGTTVSGRPAELANVENIVGLFINTLP
                     LRITIDDDTTIAPLLRRMQEDQLDLRRFEFTPLVDIHRHSEVPGDQSLFDSILVFENY
                     PVGEAVHSADELLDIGHITTIEHTNFPLTLIVEPSDKLAIRLSYDATLFESVAIQRTL
                     QHLQRVIHGVLSQPDVPLSRLPILGEQERNQILHEWNPAPANYPRNLCLHQIFERQVK
                     AHPNRVAVIAGDDEISYDELNRRANKLARVLIDAGVKDQDFVTVALDRSVNMIVSVLA
                     ILKAGGAYVPVDPEYPAERITFMLEDTKAPVMIATSPQLERIQPIIDATGAHAKLVLL
                     DRDAEIIAAKAADNMRTAMLEHPSRHAYVVYTSGSTGKPKGVLVNQLAVIRLVLDANY
                     FQLDEDDRVAHLSNVSFDASTFEMWAPLLNGIAMVLFNKEQVLTPDAFYNEVKRTRAN
                     VVFITTALFNVFAMTRPEIFPRLKYLMFGGEACDPNQVRRILKGYKPKHLLHVYGPSE
                     NTTFSTWYPIEHVSDWATTIAIGRSISGSTSYILDHHQQPVPIGVIGEIYVGGEGLAD
                     CYLNDPEKTAGSFVANPFSSNSKDRLYRTGDLARYTEDGQIEILGRADDQVKIRGFRI
                     ELGEVETSLTRLEQVAEAVVVVKKDKNQQKRLVAYVRTHGGADAALIRREAAKVLPKH
                     MMPSAFMVLKEFPLTQNGKINKRALPEPELDMGSGYRYVPPRTEKEKTLVHIWEQVLK
                     ADTVGVHDNFFELGGDSILSIQIISRAKRAGLHLTAKQIFDYQTIAELAEAAMELQDI
                     ILAEQGLVEGEAPLTPVQQWFFEQDVADAHHFNQSVMLKLDRSVTPDLLRSAWEAVLW
                     QHDALRMRYTRQESGRWLQTFRPSEHAETLLSEVFEVVNLGGLPVALRGEQITQLAGQ
                     VQAELSLAKGPLVRALYFDVGRDQPARMLVVIHHLVVDVFSWRILMEDLQTALQQLQK
                     GERVDLGSKTTSFRQWGEALTTLANSAELDADYEFWRGQLRHKPLRLPQDLQGKNQVS
                     SVKTVERRLDVSTTKRLLTETSRCYRTEINDLLLTALARSVSAWTGADSVSFDLEGHG
                     REYVADAIDVSRTVGWFTSI"
BASE COUNT          807 a         1316 c         1214 g          859 t
ORIGIN      
        1 taaatcgacg tgaaccagcc taccgtgcgc gacacatcaa tggcatctgc cacgtattcg
       61 cgaccgtggc cttccagatc gaaactgacg ctgtcggcgc cggtccaggc cgatacgctg
      121 cgagccagtg ctgtcagcag cagatcgttg atctcggtgc ggtaacagcg gctggtttct
      181 gtcagcaggc gtttggtggt ggacacatcc agccggcgtt ccaccgtttt cacgctggac
      241 acctgattct tgccctgcag atcctgcggc aaacgcagcg gcttgtgccg caactggcca
      301 cgccagaatt cgtaatcggc atccagttcc gcgctgttgg ccagcgtggt caacgcctcg
      361 ccccactggc ggaacgacgt ggtcttgctg ccaagatcga cgcgctcgcc tttctgcagc
      421 tgttgcagcg cggtctgcag gtcttccatc agaatgcgcc aggagaatac gtccaccacc
      481 aggtgatgga tcaccaccag catccgtgcc ggctgatcgc gccccacatc aaaatacagt
      541 gcgcgcacca gcgggccctt cgccaggctc agttccgcct gcacctgccc cgccagctgg
      601 gtgatctgtt cgccgcgcag tgccaccggc agtccgccca gattcaccac ttcgaacact
      661 tccgacagca aggtttcagc atgctcggag ggacggaatg tctgcagcca gcgcccgctt
      721 tcctgccgcg tgtaacgcat ccgcaacgca tcgtgctgcc acagcaccgc ttcccaggca
      781 ctgcgtaaca gatcgggcgt cacgctgcgg tcgagcttca gcatcactga ctggttgaaa
      841 tggtgcgcgt cggcaacatc ctgttcgaag aaccactgct gcaccggtgt caatggcgct
      901 tcgccttcca ccaggccctg ctccgccaga atgatgtcct gcagttccat cgcggcttct
      961 gccagttccg cgatggtctg ataatcgaat atctgcttcg cggtcagatg cagaccggcg
     1021 cgctttgcac ggctgatgat ctggatactg agaatggaat cgccgcccag ctcgaagaag
     1081 ttgtcgtgca cgccgacggt gtcggccttg agcacctgct cccagatatg aaccagggtt
     1141 ttttccttct ccgtgcgcgg cggcacgtag cggtagcccg aacccatgtc cagttccggc
     1201 tccggcaatg cgcgcttgtt gatcttgccg ttctgggtga gcggaaattc cttcagcacc
     1261 atgaacgccg acggcatcat gtgcttgggc agcactttcg ccgcctcgcg ccggatcagc
     1321 gccgcatcgg cgccgccgtg ggtgcgcaca tacgccacca gccgcttctg ctggttcttg
     1381 tccttcttca ccaccacgac cgcttcggcc acctgctcca gacgggtcag gctggtttcc
     1441 acttcaccca gctcgatgcg gaagccacga attttcacct gatcgtcggc gcggcccaga
     1501 atttcgatct ggccatcctc ggtatagcga gccaggtcgc cggtgcggta caggcgatcc
     1561 ttgctgttgc tggagaacgg attcgccaca aaactgcccg ccgttttttc cgggtcattc
     1621 aggtaacaat ccgccaggcc ttctccaccc acataaattt cgccgatgac accgatcggt
     1681 accggctgct ggtggtggtc caggatgtac gaggtggaac cggaaatact gcgaccgatg
     1741 gcaatggtgg tcgcccagtc gctgacatgc tcgatgggat accaggtgga aaacgtggtg
     1801 ttttccgacg gcccgtacac gtgcagcaga tgcttcggct tgtagccttt cagaatgcgg
     1861 cgcacctggt tcggatcgca ggcttcgccg ccgaacatca ggtatttcag acgcgggaaa
     1921 atttccggcc gggtcatggc aaacacgttg aacagcgccg tggtgatgaa caccacattg
     1981 gcgcgggtac gcttcacttc gttgtagaag gcatccggcg tcagcacctg ttccttgttg
     2041 aacagcacca tggcgatgcc gttcagcagc ggcgcccaca tttcaaaggt ggaggcatcg
     2101 aaggacacgt tggacagatg cgcaacgcga tcatcctcgt ccagctggaa gtaattggca
     2161 tccagcacca gacggatcac cgccagctgg ttcaccagca cgcccttggg cttgccggtg
     2221 gaaccggacg tgtacaccac gtaagcgtgc cgcgacggat gttccaacat ggcggtgcgc
     2281 atattgtccg ctgccttcgc ggcaatgatc tcggcgtcac gatccagcag caccagtttg
     2341 gcgtgggcac cagtggcgtc gatgatcggc tggatgcgtt ccagctgcgg gctggtggca
     2401 atcatcaccg gcgctttcgt atcctccagc atgaaggtga tgcgctcggc gggatattca
     2461 gggtccaccg gcacgtaggc gccgcccgcc ttcagaatcg ccagcacgct gacgatcatg
     2521 ttgacggaac ggtccagcgc cacagtgaca aaatcctgat ccttcacacc ggcgtcgatc
     2581 agcacccggg ccagtttgtt ggcgcgccgg ttgagttcgt cgtaactgat ttcgtcgtca
     2641 cccgcgatga cggccacccg gttcggatgg gctttcacct gacgctcgaa aatctggtgc
     2701 aggcacagat tgcgcggata gttcgccggc gccggattcc attcgtgcag gatctggtta
     2761 cgctcctgct ctcccaggat cggcagacgc gacagcggca catccggctg cgacagcacg
     2821 ccatgaatca cccgctgcaa atgctgcagc gtgcgctgga tggcaacgct ttcaaacagg
     2881 gtggcgtcat aggacaggcg gatcgccagt ttgtccgacg gctccacgat cagcgtcaac
     2941 gggaagttgg tgtgttcgat ggtggtgatg tggccgatat ccagcagctc gtccgcgcta
     3001 tgaacagcct cgcctaccgg atagttttcg aagaccagaa tgctgtcgaa cagcgactgg
     3061 tcgccgggca cttccgaatg gcggtgaatg tccaccagcg gcgtgaattc aaaacggcgc
     3121 aggtcgagct ggtcttcctg catgcgtcgc agcaacggcg caatggtggt gtcgtcgtcg
     3181 atggtgatgc gcagcggcag cgtgttgatg aacaggccga cgatattttc cacgttggcc
     3241 agttccgccg gacgccccga cacggtggtg ccgaacacca catctttctc gccactgtag
     3301 cgggccagca acacacccca cgcagcctga attaccgtgt tcagcgttac gtgctgttcg
     3361 cgcgcaaaat tctgcaggcg ggaggtcagc gtttcgtcca gtgtcaggtg cgcttcgtcg
     3421 aacaccttgt cgtggctgtt ggaacggggc ttcttgttgg gcagcggtgt cgccgcctcg
     3481 aagccggcca gatacaactt ccagaattgc agcgcatcgc ccttgtcggc acgttccaga
     3541 taggcaatga aatcctcata ggagccaggc tgatggaact gtggctgcac gcccttggcc
     3601 aacgcgccat aggtctgaaa cacctcgccc atcaccagcg gcagcgacca gccgtccagc
     3661 acgatgtgat ggaaactcca gatcagtcgc gagcgatgcc ccggcagatc cagccacgcc
     3721 agccgcatca ggccaggctt gtcgaaatcg aaaccgcgct gacgatcctt ctccagccac
     3781 acattcaaac ggtgctgctc ctgcaccgga tcggtgttgc gccagtcgaa ttcgtttact
     3841 tccatgtcga tgcggttatg cacgatctgc agcgggcgat ccagatcctg ccacacgaat
     3901 gcactgcgca gcgcggggtg acggttcacc acaaactgcc acgcatgttt cagcagatcc
     3961 cggtccacgc tgccgatcac ttccacgctc agctgttcga aataaacgcc ggcgtcattg
     4021 tcgaacaggc tgtggaacag catgccttcc tgcatcggcg acagcggata caccgacacg
     4081 atgccacgct gcttgcccac cagacggtcg atctgctgct ggtccagctt tgccagcggg
     4141 aaatctgccg gggtgtaacc gaatacgtca ggctgggtac aatgctggat gatctg
//
LOCUS       DASUEG010000179         5612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_784983, whole genome shotgun sequence.
ACCESSION   DASUEG010000179 DASUEG010000000
VERSION     DASUEG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784983"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2772
                     /locus_tag="VFX11_03420"
     CDS             <1..2772
                     /locus_tag="VFX11_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4937696.1"
                     /translation="DHWLHDVAASQHNWDGFRELMRCSLERINEEAEMHLRELAGPQL
                     CREMAELTIYASNQLHFEKPDHALLQTQPGSIAHRKQQWNLFLTQNRTIRSRADKTWG
                     FPAETHFSSKAEKELAKAYKQRVQAVLQTLSAQETTLLPLVLDFINAPPPELDDQEWQ
                     LLSDLVLVLYYAAAHLKLVFQQRKTVDFSEIALAALDTLGNPDQPGEAALALDERIQH
                     ILVDEFQDTSFIQVNLLEKLTAGWTPGDGRTLFLVGDPMQSIYAFRKADVGLFLRLWQ
                     QNLLGNVSVESLQLRMNFRSSPAVLDWVNAHFRQIFPERDDARLGAVTFAHSQAGKIR
                     KEGDATSAQLFIARDDVGAAAQAEAQWIAQRILALEPGQSVAVLVKSKSHIAWIAQAF
                     RQHDIAYQAVDIEKLNDSQIIQDLLSVYRAYLAPGDKVAWFALLRGPWCGLTLKELQL
                     VAAADAHPWRALEKLVTAPQTDELDAEMRARIDHIHATFQRAYQQRYQQPFAEALREL
                     VLQLGIPSTANSEEEVDAIELFFSMLTEVEETASTPTPDAITTRLEKLFVPAAPPQPG
                     KSMVQIMTMHKSKGLEFDVVFLPQLHRTGQADDKPLILVDKQTAVLDDVQEIFMAPRP
                     NTETARANSVYSYLWKQKQQRTKNEAVRLLYVACTRARKQLHLSACLTEDDKQQVRKP
                     RSGSLLAVIWPVLEQDANRHPIEQAPAPATHRPLRRLSLRPATGAVASVASATSGLTT
                     AITAAETEESSQFRRHAGTLVHKLFEQWVRFPAFISTTISDTVRERWRIQLQALGVDD
                     AGLDPALDIVTRAVSAVARNTGRMEWLFTQPHEQSSAEFRLSVQIDGQPQHLTIDRTF
                     VHAGTRYIIDYKLSEPDADPAAFLTAETEKYRPQLQAYRQALAAQENLPARLYLYFPL
                     LDHLQEIED"
     gene            2774..3682
                     /locus_tag="VFX11_03425"
     CDS             2774..3682
                     /locus_tag="VFX11_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX4937697.1"
                     /translation="MPISFEGKLVVATSSRALFDLSESHNIFNTQGLDAYRQYQIEHE
                     DAPLNPGEAFPLVKKLLAINEKLPGEHKVEVILLSRNSADTGLRVFNSIEHWKLPITR
                     AAFSGGKSPYRYVEAFGAHLFISTNAEDVRNALELGYAAASIIAPARTSSAPESTELK
                     IAFDGDAVLFSDESEQVYQSQGLEAFAASERAAAKTPMRGGPFKPFLQALHNLQSQFS
                     EDSCPIRTALVTARSAPAHERVVRTLRDWNIRIDESLFLGGLDKGVFLKAFGADVFFD
                     DQEGHCLSASQHVAAGHVPHGVVNRK"
     gene            complement(3698..4039)
                     /locus_tag="VFX11_03430"
     CDS             complement(3698..4039)
                     /locus_tag="VFX11_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937698.1"
                     /translation="MPEPSFANAGQVDMHSVGATANAVPLISFSLSASTDAMRGNSVD
                     FAASYRSVGLNVVTQQLGYNEVSTEFSVNAPRVKSLDFSDSIREKVENCPEKMLDKLI
                     VDGAVGLNYSW"
     gene            4442..4945
                     /locus_tag="VFX11_03435"
     CDS             4442..4945
                     /locus_tag="VFX11_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4937699.1"
                     /translation="MLRNILYATDLGLFGPFILEHVGELAKRHNARVTVVHAIEPVSI
                     FADALLATYVPQESMSALREHGYDAVLATIRARVRQAFHEDFIDFNGSTDWIADVQVI
                     GGDPAQVILDQAELSHADLIVMGSHGGRSSSQTAIGSVASKVLQLSRVPIYLVPTLST
                     PLPAIAL"
     gene            5186..>5612
                     /locus_tag="VFX11_03440"
     CDS             5186..>5612
                     /locus_tag="VFX11_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4937700.1"
                     /translation="MKLQQLRYIWEVARHDLNVSVTAQSLYTSQPGISKQIRLLEDEL
                     GVEIFSRSGKHLTRVTPAGEAILRIAGEILRKVDSIKQVAQEFSDESKGSLSLATTHT
                     QARYALPPVIETFIHKYPEVALHMHQGTPIQISEMAADGT"
BASE COUNT         1242 a         1665 c         1519 g         1186 t
ORIGIN      
        1 gatcactggc tgcatgatgt ggcggcgagc cagcacaact gggatggatt ccgcgagctg
       61 atgcgttgtt cactggaacg catcaatgaa gaagcggaaa tgcacctgcg cgaactggcg
      121 gggccacagc tgtgccgtga aatggctgaa ctcaccattt atgccagcaa ccagctccat
      181 tttgaaaaac cggaccatgc gctgctgcaa actcaacccg gttccatcgc ccatcgcaag
      241 cagcagtgga atttgtttct gacccagaat cgaaccatcc gctcccgcgc ggacaaaacc
      301 tggggctttc ccgccgaaac ccatttttcc agcaaggccg aaaaggagtt ggccaaggct
      361 tacaaacaaa gggtgcaggc agttctgcaa acgctgtcgg cccaggaaac cacgctgctg
      421 cccctggtgc tggattttat caacgcgccg ccgccggaac tcgatgacca ggaatggcaa
      481 ctgctgtcgg atctggtgct ggtgctgtat tacgcggcgg cgcatctgaa gctggtgttc
      541 cagcaacgca agactgtcga tttcagcgaa attgcgctgg ccgcgctcga tacccttggc
      601 aatcctgatc aacccggcga agccgcgctg gcgctggacg aacgcatcca gcacattctg
      661 gtggatgaat tccaggacac gtccttcatt caggtcaatc tgctggagaa gctcacggcc
      721 ggctggacgc ccggcgatgg tcgcaccctg tttctggtcg gcgatcccat gcaatcgatt
      781 tacgccttcc gcaaagccga tgtgggcctt ttcctgcgtt tgtggcagca gaacctgctg
      841 ggcaacgtga gtgtggaatc cttgcagttg cgcatgaatt ttcgttcttc tccggcggtt
      901 cttgactggg tcaacgcgca tttcagacag atttttcccg agcgcgatga cgcccgtctg
      961 ggtgccgtca ccttcgccca ttcccaggcc ggcaaaatcc ggaaggaggg cgacgcaacc
     1021 tccgcgcagc tgttcatcgc ccgtgatgat gtcggcgcgg cggcgcaggc agaagcccag
     1081 tggattgccc aacgcattct ggcactggag ccgggccagt ccgttgcagt gctggtgaaa
     1141 agcaaatccc acattgcctg gatcgcgcag gcatttcgcc agcatgacat tgcctatcag
     1201 gccgtcgaca tcgaaaaact taacgacagc cagatcattc aggatctgct gtcggtgtat
     1261 cgcgcctatc ttgcgccggg tgacaaggta gcctggtttg cgctgctgcg cggtccctgg
     1321 tgtggtctga cattaaaaga gctgcaactg gtagctgcag ccgatgccca cccctggcgc
     1381 gcactggaaa agctggtgac cgcgccacaa acagatgaac tggatgcgga aatgcgcgcc
     1441 cgcatcgacc atattcatgc cacgttccag cgtgcttacc agcagcgtta ccagcagccg
     1501 tttgcagaag cgctgcgtga actggtcctg cagctgggaa ttccctccac cgcgaatagc
     1561 gaagaggaag tggacgcgat cgaactgttt ttctccatgc tgacggaggt ggaggaaacg
     1621 gcatccaccc cgacgcccga cgccattacc acccggctgg aaaagctgtt cgtccccgcc
     1681 gctccgccgc aacccggcaa atcgatggtg cagatcatga ccatgcacaa atcgaaaggc
     1741 ctggagttcg atgtcgtctt cctgccgcag ctgcatcgca ccgggcaggc ggatgacaag
     1801 ccgcttattc tggtggacaa gcaaaccgcg gtgctggatg acgtgcagga aatcttcatg
     1861 gcgccaaggc ccaataccga aacagcgcgc gccaactcgg tttactccta tctctggaag
     1921 caaaaacagc aacgcacaaa aaatgaagcg gtgcgtctgc tttacgtggc ctgcacccgc
     1981 gcccgcaagc aactgcacct gtcggcctgc ctgaccgaag acgacaagca acaggtacgc
     2041 aaacccagat ccggcagcct gctggcggtg atctggcccg tgctggagca ggatgccaat
     2101 cgccacccga ttgaacaggc tcccgcgcca gcgacgcatc gtcccttgcg acgtttgtcc
     2161 ctgcgtcctg caaccggagc agtggccagc gttgcaagtg cgacttccgg cctgaccacc
     2221 gcgatcaccg cggctgaaac tgaggaatcc agccagttcc gccgtcatgc cggcaccctg
     2281 gttcacaaac tgtttgagca atgggtccgc tttccggctt tcatcagcac aaccatttcc
     2341 gatacagtgc gggaacgctg gcgcatccag ctgcaggcgc tgggtgtgga tgatgcgggg
     2401 ctcgacccgg cccttgatat cgtgacgcgc gctgtatcgg cggtcgcccg caacaccgga
     2461 cgtatggagt ggctgttcac ccagccgcat gaacagtcaa gcgcggaatt ccgtctcagt
     2521 gtgcagatcg acggccaacc ccagcacctc accatcgatc gcaccttcgt gcacgcgggc
     2581 acccgctata taatcgatta caagctgtca gaaccggatg ccgatcctgc cgcattcctg
     2641 acggccgaga ctgaaaaata caggccgcaa ctgcaggcct accggcaggc gctggcggca
     2701 caggaaaacc tgccggcccg cctgtatctt tattttccac tgctggacca cttgcaggaa
     2761 atcgaggact gaaatgccca tttcatttga aggaaaactg gtggttgcca cctcgtcccg
     2821 cgcgctgttc gatctgagcg aaagccacaa catctttaac acccagggac tggatgctta
     2881 ccgtcagtac cagatcgaac acgaagatgc gcccctgaat ccgggcgagg cgtttcctct
     2941 ggtcaaaaaa ctgctggcta tcaacgagaa attgccgggc gaacacaaag tggaagtgat
     3001 cctgctgtcg cgcaattccg ccgataccgg tctgcgggtt ttcaattcca tcgagcactg
     3061 gaaactgccc atcacccgtg cggccttttc cggcggcaaa agtccctatc gctacgtcga
     3121 agccttcggc gcgcatctgt ttatttccac caacgccgag gatgtgcgca acgcactgga
     3181 actgggttat gccgctgcca gcatcatcgc accggcacgc acgtccagcg caccggaatc
     3241 caccgaactg aagatcgcct ttgacggtga tgccgtgctg ttttccgacg aatccgaaca
     3301 ggtttatcaa tcccagggac tggaagcgtt cgccgcatcc gagcgcgccg ccgccaaaac
     3361 gccgatgcgc ggcggcccgt tcaaaccctt cctgcaggca ttacacaatc tgcaatcgca
     3421 gttttccgaa gacagttgcc ccatacgcac tgcgttagtc acggcgcgct cggcaccggc
     3481 gcacgaacgg gtagtgcgca ccttgcgcga ctggaacatc cggatcgatg aatcactgtt
     3541 tctgggcggg ctagataaag gcgtgttcct gaaagcgttt ggcgcagatg tattctttga
     3601 tgatcaggaa gggcattgcc tgtcggcgag tcagcatgtg gccgcgggtc acgttcctca
     3661 cggcgtcgtc aacaggaaat aacgtcagaa atagagctta ccaggagtag ttcaggccca
     3721 ctgcgccgtc gacgatcagc ttgtcgagca ttttctcggg gcagttttcc actttctcgc
     3781 ggatggaatc agaaaaatcc aggcttttca cgcgcggtgc gttcacggaa aattccgtgg
     3841 acacttcgtt gtagcccagt tgctgcgtca ccacattcag acctaccgac cggtaggatg
     3901 cagcgaaatc cacgctgttg ccgcgcatgg cgtcagtact ggcagaaagg ctgaaggaaa
     3961 taagcggcac tgcattggca gtggcaccca cagagtgcat gtcaacctgg ccagcgttgg
     4021 cgaatgaagg ttcaggcatg taattgcttg cctgtgtttc cagagtagtg agcaacagca
     4081 gacatcctgc gacggccgaa actgctttta ctgccacgga gcggactttc ataattcaac
     4141 caacttcgtt attgaaagtg ttattggatg attgaagcgt agtagcggca actggcctaa
     4201 accatgttgg aattttcagt ttgtggcagt tttcccgctt tcagtcctta aatattctgc
     4261 tggcatattc gttgctttgt ttttgctgat gccagttcct cgcaagaacg tttcctctcc
     4321 tttttcctgt ttctcttttc tcgcgtattc gtcccatatc gatcaggcaa atcacatcaa
     4381 gcctgctatt ctcaactatg gtcaacgttt gcccattccg ccgttaacgt gctgtaaata
     4441 aatgttaaga aatattcttt atgccaccga cctggggttg tttggcccgt ttattctgga
     4501 acatgtggga gaactggcca aacgccacaa cgcacgggtc accgtggtgc atgcaatcga
     4561 gccagtgagc atttttgcgg atgcgctgct ggctacctac gtgccgcagg aatccatgtc
     4621 ggcactgcgc gaacacggtt atgacgctgt gcttgccact atccgtgcgc gggtaagaca
     4681 ggcctttcat gaagacttca ttgacttcaa cggctccacc gactggattg cagatgtgca
     4741 ggtcatcggc ggcgatcccg cccaggtgat tctggaccag gccgaactgt cccacgccga
     4801 cctgattgtc atgggcagtc acgggggacg ctcctccagc cagaccgcca tcggttccgt
     4861 ggcctccaag gtattgcaac tgtcccgggt gcccatttac ctggtgccca ccctgtctac
     4921 ccctctgccg gccatagccc tctagggcag tgcccatatt ccagccaccg ccggaatcct
     4981 gtttttctgc accggatcgg gaatatccat agacgaattc gatattctct tctaacttta
     5041 actggccatt aagggaataa atttaacaat ccggttattg atgggttggt aaatcagaaa
     5101 ttctgtccta ttctgcaatg gaatagacgc ctcatttttt ataaccccac aacgagaatt
     5161 caaaccaggg ggagggaagg aaagaatgaa actccagcaa ttgcgttaca tctgggaagt
     5221 ggcgcgacac gatctgaacg tctccgttac tgcacagagc ctgtatacat cacagcccgg
     5281 catcagcaag cagatccgct tgctggaaga cgagctgggt gtagaaatat tctcccgcag
     5341 tggcaagcac ctgacccgcg tgactccagc tggcgaagcc atcctgcgta tcgccggcga
     5401 gatcctgcgc aaggtcgaca gcatcaaaca ggtagcccag gaattcagcg acgagagcaa
     5461 gggcagcctg tccctggcca ccacccacac gcaggcgcgc tatgcgttgc cgccggtgat
     5521 cgaaaccttc atacataaat atccggaagt cgccctgcac atgcaccagg gcacgccaat
     5581 ccagatttcc gagatggcgg ccgacggcac cg
//
LOCUS       DASUEG010000180         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1552705, whole genome shotgun sequence.
ACCESSION   DASUEG010000180 DASUEG010000000
VERSION     DASUEG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552705"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFX11_03445"
     CDS             complement(<1..323)
                     /locus_tag="VFX11_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937701.1"
                     /translation="MSQAHLSLRVKVITVVILCSIALLGSLYLHNQSQAREQLQAQRQ
                     VMQYLSVANQIQSLVIRTQQLRASTDAATNRQLLQKLQDNNSQLATVASLSFSSANPL
                     LENVTS"
     gene            complement(429..1502)
                     /locus_tag="VFX11_03450"
     CDS             complement(429..1502)
                     /locus_tag="VFX11_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4937702.1"
                     /translation="MPHSEALLKLESVRCAFDDGTVSNTVVDDLSFHVNKGDIACLLG
                     PSGCGKTTTLRAIAGFEPVTNGVIYLNSKPVSTPVHRVPPEQRHLGMVFQDYALFPHL
                     TLWENVCFGLRKLSRAEKQKVAARVLEMVGLNQISHRYPHEISGGQQQRVALARALAP
                     EPALILLDEPFSNLDVDLRRRLALEVRDILKQQGTTALMVTHDQEEAFAMADKVGVMQ
                     NGQLQQWDSPFTLYHEPVNRFVAGFIGQGRFIPGKALSPEQVSTELGLIQGNRAYHWD
                     KGTAVQVLLRPDDVQPDPAGPLRALILNKTFTGATSLVQLKLPSGAVVDALFPSNAEE
                     QPGDFVGIRLDVSHLVAFVAEAG"
     gene            complement(1523..2437)
                     /gene="argF"
                     /locus_tag="VFX11_03455"
     CDS             complement(1523..2437)
                     /gene="argF"
                     /locus_tag="VFX11_03455"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX4937703.1"
                     /translation="MATRHFLTLLDIEPATLEKLLDRAIELKAMLRKGVQHEPFRNKV
                     LGMIFEKSSTRTRVSFESAMLQCGGGAIFLSPRDTQLGRGEPVADTARVLSRMVDIIM
                     IRTFEHEKIETFARYSQVPVINALTDTYHPCQLLADIQTYIEHRGSIRGKIVAWVGDG
                     NNMCHSYIEAARQFGFQLRIACPEGFEPNANLVATHSDRVTIVRDPKAAVEHAHLVAT
                     DVWASMGQEDEQDHRAGAFAGYQVSPALMDLAAKDALFMHCLPAHRGEEVSEDMLDDP
                     RSVVWDEAENRLHAQKALMEYLLGYWKP"
     gene            complement(2493..3692)
                     /locus_tag="VFX11_03460"
     CDS             complement(2493..3692)
                     /locus_tag="VFX11_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4937704.1"
                     /translation="MATMGLMSTYRQMDVAFEKGEGVWLFDTDGNRYLDAISGIAVCG
                     LGHCHPKITRVIQEQAARLVHTSNLYRITNQELLGKKLCEVSGMDAVFFGNSGAEANE
                     CAIKIARLYGHGKEIDKPTIIVMDHSFHGRTLATLTATGSRKVQAGFEPLVQGFVRAP
                     YNDLDAVRTIASNNPKVVAVLVEPVQGEGGINIASDAYLQGLRTICDENGWLLMLDEI
                     QTGNGRTGTYFAYQQMGFMPDVVTTAKGLGNGLPIGACLARGPAATTFKPGNHGSTYG
                     GNPLCCGVALAVVDTIVSENLPARAKALGERILQGFRAQLDGAEYISEIRGKGLMIGI
                     ELKEPCAELVMLAKTQGLLINVTSDKVIRLLPALTMSDSEADQLVSTLSKLIKIWAAD
                     ERTKPRR"
     gene            3924..>4153
                     /locus_tag="VFX11_03465"
     CDS             3924..>4153
                     /locus_tag="VFX11_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4937705.1"
                     /translation="MNPIELGLFSSRIESICDDMGAALRRAAFSPNIKDRLDFSCAIF
                     AASGELTAQAAHIPVHLGSMAYAMRSIVSGIA"
BASE COUNT          709 a         1215 c         1367 g          862 t
ORIGIN      
        1 gaggtgacgt tttccagcag cggattggcg ctgctgaaac tcaggctggc gaccgtggcc
       61 agctggctgt tgttgtcctg cagtttttgc agtagctggc ggttggtggc ggcatcggtg
      121 ctggcgcgca gctgctgggt ccggatcacc aggctctgga tctggttggc taccgacagg
      181 tattgcatca cttgccgctg ggcttgcaat tgttcacggg cctggctctg gttgtgcagg
      241 tacaggctgc ccagcagggc aatgctgcac aggatgacga ccgtgatgac cttgacgcgg
      301 agggataggt gggcctgtga catcgggcgc ttcgctcaaa aaccagtgac tgagttttaa
      361 gcatagcagc cggccggagg ggtggctgac gggccggggc cgggcagggg ttcgggaagg
      421 gcgggcgcct agcctgcttc ggcgacgaag gccaccaggt ggctgacgtc cagccggatg
      481 cccacgaagt cgcccggctg ttcctcggcg ttgctgggga agagggcatc caccacggcg
      541 ccggacggca gtttcagctg taccagggac gtggcaccgg tgaaggtctt gttcaggatc
      601 agggcccgca gggggccggc gggatcaggc tgtacatcgt cgggccgcag cagcacttgc
      661 accgcggtcc ccttgtccca gtgataggcc cggttgccct ggataagccc cagttcggtc
      721 gatacctgct ccggtgacag ggccttgccc ggtatgaagc ggccctggcc aatgaatccc
      781 gccacgaagc ggtttaccgg ctcgtgatac agggtgaaag gcgaatccca ctgctgcagc
      841 tggccgttct gcatcacgcc caccttgtct gccatcgcga aggcttcttc ctggtcgtgg
      901 gtgaccatca gggccgtggt gccctgctgc ttgaggatgt cgcgcacctc cagcgccagg
      961 cggcggcgca ggtcgacgtc caggttggaa aagggttcgt ccagcaggat cagggccggc
     1021 tccggtgcca gggcgcgggc gagggcgacc cgctgttgct ggccgccgga tatttcatgg
     1081 gggtagcgat ggctgatctg gttcagcccc accatttcca gcacgcgcgc cgccactttc
     1141 tgcttttccg cgcgggacag tttgcgcagg ccgaagcaga cgttttccca cagggtcagg
     1201 tgggggaaga gggcgtagtc ctggaacacc atgcccagat ggcgctgttc cggcggcacc
     1261 cggtggacgg gggtggagac aggtttgctg ttcaggtaga tgacgccatt ggtgaccggt
     1321 tcgaaaccgg cgatggcgcg cagggtggtg gtcttgccgc agccgctggg gcccagcagg
     1381 caggcgatgt cgcccttgtt gacgtggaag ctgagatcgt ccaccaccgt gttgctgacg
     1441 gttccgtcat cgaaggcgca gcgcaccgat tccagtttaa gcaaggcttc cgagtggggc
     1501 atcgactgcg tttccccgcc gctcagggct tccagtagcc cagcagatat tccatcaggg
     1561 ccttctgcgc gtgcaggcgg ttttccgctt cgtcccacac caccgagcgc gggtcgtcca
     1621 gcatgtcctc gctgacttcc tcaccgcgat gggctggcag gcagtgcatg aacagcgcgt
     1681 ctttcgccgc cagatccatc agtgccggtg acacctgata gcccgcaaac gcaccggcgc
     1741 ggtgatcctg ttcgtcttcc tggcccatgc tggcccagac gtcggtggcc accaggtggg
     1801 cgtgttcaac cgccgctttc ggatcgcgca cgatggtgac gcggtcgctg tgggtggcca
     1861 ccagattggc gttgggctca aacccttccg ggcaggcgat gcgcagctgg aagccgaact
     1921 ggcgcgccgc ttcgatgtag gaatggcaca tgttgttgcc gtcgcccacc caggccacga
     1981 tcttgccgcg aatggagccg cggtgttcga tgtaggtctg gatgtccgcc agcagctggc
     2041 agggatggta ggtgtcggtg agggcgttga tcactggcac ttgcgaatag cgggcaaagg
     2101 tttcgatctt ctcgtgctcg aaggtgcgga tcatgatgat gtcgaccatc cgcgacagca
     2161 cacgcgcggt gtcggccacc ggctcgccac ggcccagctg ggtatcgcgg ggtgacagga
     2221 atattgcacc gccgccgcac tgcagcatgg cgctttcgaa cgacacgcgg gtgcgggtgg
     2281 aggatttttc aaaaatcatc cccagcacct tgttccggaa tggctcgtgc tgcacgcctt
     2341 tgcgcagcat ggctttcagt tcgatggccc ggtccagcag tttttccagc gtggccggtt
     2401 cgatgtccag taatgtcaga aaatgccgtg tcgccatggc gtgatcccgc aaatgctatt
     2461 ggtcaaaaaa ttaaggcgta agcaatttca tttcagcggc gcggtttggt gcgctcgtct
     2521 gcagcccaga tcttgatgag cttcgacagt gtgcttacca gctggtccgc ttcgctatcg
     2581 ctcatggtga gggccggcag caaccggatc accttgtctg atgtaacatt gatcagcagg
     2641 ccctgtgtct tggccagcat caccagttcc gcgcagggtt ctttcagttc gatgccgatc
     2701 atcaggccct tgccgcgtat ttcactgatg tactcggcgc catccagttg ggcgcggaag
     2761 ccctgcagga tacgctcgcc cagtgccttg gcccgggccg gcaggttttc cgatacgatg
     2821 gtgtccacca ccgccagcgc gacgccgcag cacagcggat tgccgccata ggtggaaccg
     2881 tggttgccgg gtttgaacgt ggtggccgcc ggaccccgtg ccaggcaggc gccgatgggc
     2941 aggccgttgc cgaggccttt cgcggtggtg acgacatcgg gcataaaacc catttgctgg
     3001 taggcgaaat aggtgccggt gcggccgttg ccggtctgga tctcgtccag catcagcagc
     3061 cagccgtttt cgtcgcagat ggtgcgcagg ccctgcaggt aagcgtcgga cgcgatgttg
     3121 atgccgcctt caccctgtac cggctcgacc agtaccgcaa ccaccttggg attgttgctg
     3181 gcgatggtgc gcacggcatc cagatcgttg tagggcgcac gcacaaaacc ctgcaccagc
     3241 ggctcgaagc cggcctgcac cttgcggctg ccggtggcag tgagggtggc cagggtacgg
     3301 ccgtggaagg aatggtccat cacgatgatg gtgggcttgt cgatttcctt gccgtggcca
     3361 tacaggcgcg cgatcttgat ggcacactca ttggcttcgg cgccggagtt gccgaaaaag
     3421 accgcgtcca tgccggatac ttcacacagc ttcttgccca gcagttcctg gttggtgata
     3481 cggtaaaggt tggaggtgtg caccagtcgc gccgcctgtt cctggatgac gcgcgtgatt
     3541 ttgggatggc agtggcccag tccgcagact gctatgccgc tgatcgcgtc cagataccga
     3601 ttgccatcgg tatcgaacaa ccagacgcct tcgccttttt caaaggctac atccatctgc
     3661 cggtaggtgg acatcaaacc cattgttgcc atagtgcttt accgatcctt aaccccgtgc
     3721 cacaaaataa aaaaggcagc gattgctgcc gtcggaaagt caatcatagt cccaatccgg
     3781 ggtgggtgca atcagggggc ggccaggggg ggcgcaggtg cctcaagtgc ctggaaaagg
     3841 gcggatttcg ccgttgaccg ggtggttgtg cagggccaac atgggggtcc gctgatcact
     3901 gttttcaagg cattgaacga accgtgaatc ccatcgaact gggcctgttt tccagccgca
     3961 ttgaatccat ctgcgacgat atgggcgccg ccctgcgtcg tgccgcgttt tcccccaaca
     4021 tcaaggaccg gctggatttc tcctgcgcaa tttttgctgc gagtggcgag ctgacagcgc
     4081 aggctgcgca cattcccgtg cacctgggca gtatggctta cgcgatgcgc tccatcgtga
     4141 gcgggattgc atg
//
LOCUS       DASUEG010000181         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1625033, whole genome shotgun sequence.
ACCESSION   DASUEG010000181 DASUEG010000000
VERSION     DASUEG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625033"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1135
                     /locus_tag="VFX11_03470"
     CDS             59..1135
                     /locus_tag="VFX11_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4937706.1"
                     /translation="MKILNKLTGLLGAGAKNEAKQGFVLDVSHLKILVPFNEMPDNHL
                     QEVLKIARVQSVPARKKLFKRGQEDTLVHYVLQGTVDLLDEHFNITSIKAGDAAGAHA
                     LDNETPHRLSAITTCDSLLLEFDKDRLDLLMTWEQAASGEQAGADTPEEDWMSALLES
                     ELFSKVPPAHIQQLFATFKSQQCSAGDVIVQEGQPGDRFFVIAAGRARVSRRGADGDR
                     QLAELGAGRFFGEEALIGETTRNATITMETAGKLMFLDKAEFKKLLEEPVLRSLSGPD
                     LERMRTSGQPLVLLDVRLSGEYKHLHDEGSINVPLNRLRDRMTEFAREKFYVVSANAG
                     RRSELGVYLLISAGFQAYLLRQTE"
     gene            1304..>2418
                     /locus_tag="VFX11_03475"
     CDS             1304..>2418
                     /locus_tag="VFX11_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4937707.1"
                     /translation="MALLMLCCWMGLCLPQAWASVTLTEPHFKLSLLTSVSYFEDSDN
                     AFEPDSFADPALIRQFTPARTPILRFGYTDSTLWLRLQIDNRQEATRNALLYLTRPNI
                     GLVEVYQLAPAGVLPLGKAGSQTRAVHGDIHHRTPVIALPLAPHQTSEYLIRMRSDQY
                     LNFSLQLADPASFYQEQQVQELITGIASGIVLMLFLGALFEFAKRRELNDLNFAAYAL
                     ALLMYIGSALGYLGFLWVPVTNLQTRLDALSMMLVCATGLQFTRTLLDTQKTRRHLDA
                     LLRLVLGIIALTMLACLAASPRMSTEAAMMVAMLTIPLTLYAVVLRAMDGHPPARYLL
                     PARTLVLLVGGLSAQTVYAQLAFGLETTWLMLGALFL"
BASE COUNT          492 a          779 c          704 g          443 t
ORIGIN      
        1 cagccacgtc tacacttagg gcaaggaaac ggtgattcaa taccgtcaaa gacatgccat
       61 gaagattctc aataaattga ccggtctgct gggtgctggc gccaagaatg aagccaaaca
      121 gggtttcgtt ctggatgtat cgcatctgaa aatcctggtg cctttcaacg agatgccgga
      181 taaccatctg caggaagtgc tgaaaattgc ccgcgtgcag tcagtaccgg cgcgcaagaa
      241 actgttcaag cgtggccagg aggacaccct cgtgcactac gtgctgcagg gcacggtgga
      301 cttgctggac gagcatttca acattacctc gatcaaggca ggagatgctg ccggcgccca
      361 cgccctggac aatgaaacac cccaccgact gtcggccatt accacctgcg acagcctgct
      421 gctggagttc gacaaggacc ggctcgacct gctgatgacc tgggaacagg ccgccagcgg
      481 agagcaggcc ggtgccgaca cccccgagga agactggatg tcggccctgc tggagtcgga
      541 actgttcagc aaggttcccc ccgcccatat ccagcagctg ttcgccacat tcaaaagcca
      601 gcaatgcagc gccggtgatg tgatcgtgca ggaaggccag ccgggtgacc gcttcttcgt
      661 cattgccgcc ggccgcgcac gggtcagccg ccggggtgcc gacggcgacc gtcaactggc
      721 tgaactgggg gccggacgct ttttcggcga agaagccctg atcggcgaaa ccacccgcaa
      781 tgccaccatc accatggaaa ccgccggcaa gctgatgttc ctggacaagg cggaattcaa
      841 gaaactgctg gaagaaccgg tactgcgcag cctgagcggg cctgacctgg agcgcatgcg
      901 caccagtggc cagccactgg tactgctgga tgtgcggttg tcgggcgaat acaagcacct
      961 gcacgacgaa ggcagtatca atgtgcccct gaaccggctg cgggatcgga tgacggaatt
     1021 tgccagggaa aagttctacg tggtatccgc caacgctgga cgtcgaagcg aattaggggt
     1081 ctacttgctg atcagtgccg gctttcaggc ctacctgctg cggcagacgg aataggccac
     1141 caccggcctg acagggcatc gtcaaattgt ctgacaataa ccgattcatc ctgtaggaac
     1201 cggcgccaag tgggatatac ttggcccaaa attaaccaat cgcaggacgc aaagcaccgc
     1261 tctcatgagg cgacaggccc cccaccatcg atctgccacc ggaatggcac tgctcatgct
     1321 gtgctgctgg atgggattgt gcctgcccca ggcatgggcc agcgtgacgc tgacggaacc
     1381 ccatttcaag ctgtcgctgc tgaccagcgt gagttacttc gaggacagcg acaacgcctt
     1441 cgaaccggac agctttgccg accccgccct gattcgccag ttcacccccg cccgcacccc
     1501 catcctgcgc tttggctaca ccgattccac cctttggctg cgcctgcaga tcgacaaccg
     1561 gcaggaagca acccgcaacg cgctgctgta cctgacccga cccaacatcg gcctggtgga
     1621 ggtgtatcaa ctcgcccccg ctggcgtact gcccctgggc aaggccggca gccagacccg
     1681 ggccgtgcac ggcgacattc accaccgcac accggtgatc gcactgccgc tggcaccgca
     1741 ccagaccagc gaatatctga tccgcatgcg ctcggaccag tacctgaatt tctcgctgca
     1801 actggccgac cccgccagct tctatcagga acagcaggtg caggaactga tcaccggcat
     1861 tgccagcggt attgtgctga tgctgttcct gggcgcgctg tttgaattcg cgaaacggcg
     1921 cgaactgaac gatctcaact tcgccgccta tgcgctggcg ctgctgatgt atatcggatc
     1981 ggcgctgggt tatctgggtt tcctgtgggt gccagtgacc aatctgcaaa cccggctcga
     2041 cgccctgtcg atgatgctgg tgtgcgccac cggcctgcag ttcacccgca ccctgctgga
     2101 tacccagaag acgcgccgcc atctggacgc cctgctgcgg ctggtgctgg gcatcattgc
     2161 cctcaccatg ctggcctgcc tggccgcatc gccgcgcatg tccaccgagg ctgccatgat
     2221 ggtggccatg ctcaccattc cgctgaccct gtacgcagtg gtgctgcgcg ccatggacgg
     2281 ccacccgccg gcccgctatc tgctgccggc ccgcaccctg gtactgctgg tgggtggact
     2341 cagtgcgcag accgtgtacg cgcaactggc attcgggctg gaaaccacct ggctgatgct
     2401 gggcgccctg ttcctcga
//
LOCUS       DASUEG010000182         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_227056, whole genome shotgun sequence.
ACCESSION   DASUEG010000182 DASUEG010000000
VERSION     DASUEG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227056"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFX11_03480"
     CDS             complement(<1..927)
                     /locus_tag="VFX11_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937708.1"
                     /translation="MTESAQAKARQLLDAQVNFWLQELAPDRLAAIVREEIAYLYGEL
                     DRISLHEAVSAAKIKATAHRYALEMEIGGAIPELFGEIANLVYEHPAQYDTRVSDIFS
                     SRIAQEFIEKVFESGSLLDQAVRQIQQSDPFKVFLADVVLLAVKGYLLEQNRLLGTTP
                     VIGAGLRRLRAFLDERLPSLQDGIEEASHQLLERSIGNTLTLLDQLLDQGEYREQALA
                     ATLNLWDDISTWPVSRLRDFTSENDLQELMVLGYEFWREFRETDYLTSIIDAGVDFFF
                     DKYGEDSLQKIISEMGVTYEMIESEILNYSADM"
     gene            complement(1078..2058)
                     /locus_tag="VFX11_03485"
     CDS             complement(1078..2058)
                     /locus_tag="VFX11_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937709.1"
                     /translation="MAIATLAACGGGGSGGSTASSASADTQAPAPAAVMQGQFLDSAV
                     SGLYYETDSLFGYTGLNGEFSYVPGETVRFYLGSTLLGETTAQDEVTPLDLVTGTDDP
                     DKLQNILRTLQTIDEDSDPSNGIQINNIAQNYLDAYVLPINQPAVLFEASEVIQDMIA
                     AVTNGTGLKDAIDSFIHFRETLLLSRRNTDETVLLNLLGTTWDAEITSTACAGDAKAK
                     LVYSFNSLGVATTGYHRLNTNLDDETGEPICKKAGYGILFSTYETDPIFTCANQCVDS
                     DLNRVILDSDENGDIVIAMNYDPINQQISIRETRTNGDEEVTTTKVLTRR"
     gene            complement(2368..3204)
                     /locus_tag="VFX11_03490"
     CDS             complement(2368..3204)
                     /locus_tag="VFX11_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX4937710.1"
                     /translation="MRIFAGTLLPLLLLLPLSSQGSSAAPARIAIILDDIGYNRSRGE
                     QALQLPPAVTFSVIPFTPHSKTLAQKAHAQQREVLLHLPMESADSDRRLDEGGLTQQQ
                     PEAEIRSRVNAAIAAVPHAVGLNNHMGSGLTTDPQRMGWVMEEVRRTPLFFIDSMTSA
                     ESVAGSTARRLQIPSIRRDVFLDNQPDASAIDTAFRLLLQKARQDGYAVAIGHPHAAT
                     LRYLEQALPRLAAEGIELVPVSHLAHQYGRALDPPGRWQDLMRLIIAMPAPSSTRLDF
                     SR"
     gene            complement(3217..>4537)
                     /locus_tag="VFX11_03495"
     CDS             complement(3217..>4537)
                     /locus_tag="VFX11_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4937711.1"
                     /translation="TAPAQPATAAPAPQQGLLPLNELRTFADVFDRIKQAYVEEVDDK
                     TLLENAIKGMLTELDPHSAYLEPEAYQDLQISTTGEFGGLGIEVGMEDGFVKVITPID
                     DTPAQKAGVLTGDLIIKLDDTPVKGLSLNDAVKLMRGKPGSVIKLTIIRQGVDKPLEI
                     DVVRAVIQVQSVKERMLEKNYGYVRLSQFQVHTGSDLLKSLEKLKKENNGELKGLVLD
                     MRNNPGGVLQAAVDVADAFINDGLVVYTKGRLPDSDMQFSATPGDHLKGTPIVVLING
                     GSASASEIVAGALQDHHRAVIMGTTSFGKGSVQTVLPLHNNRALKLTTARYYTPGGRS
                     IQAEGIVPDIQVDNARLTTVEENDRIKEKDLTRHLINPANGDSKDAKEKTDDSKKKDD
                     KPAKPEASKGATEPPAKLSESDYQLYEALNLLKALNVLGKTVQAANR"
BASE COUNT          755 a         1265 c         1509 g         1008 t
ORIGIN      
        1 catgtctgcg ctgtaattca ggatttccga ttcgatcatt tcgtaggtca cgcccatttc
       61 gctgatgatt ttctgcagtg aatcctcgcc gtatttgtcg aagaagaaat ccactcccgc
      121 atcgatgatg ctggtcagat aatcggtctc gcggaattcg cgccagaatt cgtaacccag
      181 caccatcaat tcctgcagat cgttttcgga ggtgaaatcc cgcaggcgcg acactggcca
      241 ggtgctgatg tcgtcccaca ggttgagcgt ggccgccagc gcctgctcgc ggtattcccc
      301 ctggtccagc aactggtcca gcagggtgag ggtgttgccg atgctgcgct ccagcagctg
      361 gtgcgacgct tcctcgatgc catcctgcag gctgggcagg cgctcatcca gaaacgcccg
      421 cagccggcgc aggccggcac cgatcaccgg cgtcgtgccc agcagtctgt tctgttccag
      481 caggtagcct tttaccgcca gcaacaccac atccgccaga aacaccttga acggatcgct
      541 ctgctggatc tggcgcacgg cctggtccag caggctgccg gattcgaaaa ccttttcgat
      601 gaattcctgg gcgatgcgac tggaaaaaat atcgctgacc cgggtgtcgt attgcgcggg
      661 gtgttcgtac accagattgg cgatctcgcc gaacagttcg ggaatggcgc cgccgatttc
      721 catctccaga gcgtagcggt gcgcggtggc cttgatcttg gcggcgctca cggcttcgtg
      781 caggctgatc cggtccagtt cgccgtacag ataggcgatt tcctcgcgca cgatggcggc
      841 aaggcggtcg ggcgccagtt cctgcagcca gaaattgacc tgggcgtcca gtagttgacg
      901 ggctttggcc tgcgcggatt cggtcatggt ggcggacatt cctgctggtg gggcttgggg
      961 acagaagccc gccgagtata ccaaggttcg gcgcggggac tgcagcgtca cattccttgc
     1021 aaaaaaaagg ctgcgggtta gcgccgcagc cttggttgtt acgcgcgatg ctgtcgatca
     1081 gcggcgggtc agcaccttgg tggtggtgac ttcttcatca ccgttggtgc gggtttcgcg
     1141 aatgctgatc tgctggttga tgggatcgta gttcatggca atcacgatat cgccgttctc
     1201 gtcgctgtcg aggatcacgc gattcaggtc ggaatccaca cactggttgg cgcaggtgaa
     1261 gatgggatct gtctcgtaag tgctgaacag gatgccgtag cctgccttct tgcagatggg
     1321 ctcgccggtc tcgtcgtcga ggttggtgtt caggcgatgg taaccggtgg tggccacacc
     1381 caggctgttg aaggagtaaa ccagtttggc tttcgcgtca ccggcgcagg cagtggatgt
     1441 gatctctgcg tcccaggtgg tgccgagcag attcagcagc acggtttcat cggtgttgcg
     1501 gcgcgacagc agcagggttt cgcggaagtg gatgaaggaa tcgatggcat ccttcagtcc
     1561 ggtgccgttg gtgacggcgg cgatcatgtc ctgaatcact tcactggcct cgaacagcac
     1621 agccggctgg ttgatcggca ggacgtacgc gtccagataa ttctgggcaa tgttgttgat
     1681 ctggatgccg ttgctggggt ccgaatcctc gtcgatcgtc tgcagggtgc gcaggatgtt
     1741 ctgcagcttg tccgggtcgt cggtaccggt gaccaggtcg agcggtgtca cctcgtcctg
     1801 ggcggtggtt tcgcccagca gtgtgctgcc caggtagaag cgcacggttt cacccggcac
     1861 gtagctgaat tcaccattca ggccggtgta gccgaacagg gaatcggttt cgtagtacag
     1921 gcccgacact gcgctgtcga ggaactggcc ctgcatgacg gcggccggcg cgggtgcctg
     1981 ggtgtcggcg gatgcgctgg atgcagtgct gccaccgcta ccaccgccac cacaggcggc
     2041 aagcgtggca atagccatgg aaagtacaag gggttttgcg gctgggaaat aacggagcat
     2101 taaagcttct cctgctgaac gggacttcaa gtacacacat tgacagtcgc cttccggtcc
     2161 ggagagtcag gctgtcaaaa cctgaattga atctagacga cgatttttcg gcatgcctgt
     2221 cagcaaagtt gaccggtatc gcattattgg cgcggacaga tccggctgcg tcgattttgt
     2281 gacgcggcta gcggtattga tgaagcgggg ttgtgaaggg ggattgaggt gctagcggac
     2341 gcgtggcacg gggctggcgc gagcccctca gcgggaaaaa tccagccggg tgctgctggg
     2401 agcgggcatg gcgatgatca agcgcatcag atcctgccag cggcccggcg ggtcgagggc
     2461 gcggccgtac tggtgggcca ggtgtgacac gggcaccagc tcgatgccct ctgccgccag
     2521 ccttggcagt gcctgttcca ggtaacgcag ggtggcagcg tgggggtggc cgatggcaac
     2581 ggcgtagccg tcctggcggg ccttctgcag cagcaaccgg aatgcggtgt cgatggcact
     2641 ggcgtccggc tggttgtcca gaaacacgtc gcggcggatg gatggaatct gcaaccggcg
     2701 cgccgtgctg ccggccacgg attcggcgct ggtcatgctg tcgatgaaaa acaggggtgt
     2761 gcggcggact tcctccatca cccaacccat gcgctgggga tcggtagtca gcccgctgcc
     2821 catgtgattg ttgaggccga cggcatgggg cacggccgca atggcggcgt ttacccggga
     2881 ccgtatttcc gcttccggtt gttgctgggt gaggccgcct tcgtccagcc ggcggtcgct
     2941 gtcggcactt tccatcggca ggtgcagcag cacttcccgc tgctgggcat gggctttttg
     3001 cgccagcgtt ttgctgtggg gtgtgaaagg aatgacggag aaagtgactg ccggcggcag
     3061 ctgcagcgcc tgttcaccgc gactgcggtt gtagccgatg tcgtcgagga tgatggcgat
     3121 gcgggccggt gcggcgctgc tgccctggct cgacaggggc agcagcaaca gcaacggcag
     3181 cagggtgccg gcgaaaatgc gcaatggccg tgtccgtcag cggttggcag cctgcactgt
     3241 cttgcccagc acattcagtg ctttcagcag gttcagtgct tcgtacagct ggtaatcgga
     3301 ttccgacagt ttggcgggtg gctcggttgc acccttgctg gcttccggct tggcgggttt
     3361 gtcgtccttt ttcttgctgt cgtcggtttt ttccttggcg tccttgctgt caccatttgc
     3421 cggattgatc aggtggcggg tgagatcctt ttccttgatg cgatcgtttt cttccaccgt
     3481 ggtcaggcgg gcgttgtcca cctggatgtc gggcacgatg ccttccgcct ggatcgagcg
     3541 gccgcccggg gtgtagtagc gcgcggtggt gagtttcagg gcgcggttat tgtgcagcgg
     3601 cagtacggtc tgaaccgagc ctttgccgaa agaggtggtg cccatgatca cggcacgatg
     3661 gtgatcctgc agtgcgccgg ccacgatttc ggatgcggac gcggaaccgc cattgatcag
     3721 taccacgatc ggggtgccct tgaggtggtc gccaggggtg gcgctgaact gcatgtcgga
     3781 atccggcaga cggcccttgg tgtacaccac caggccgtcg ttgatgaagg cgtcggccac
     3841 gtccaccgcg gcctgcagca cgccgccggg gttgttgcgc atgtccagca caagcccttt
     3901 cagctcgccg ttgttttcct tcttcagttt ttccagcgat ttcagcaaat cggagcctgt
     3961 gtgcacctgg aactgggaca gccgcacgta gccataattt ttctccagca tgcgttcctt
     4021 cacgctctgc acctggatca cggcgcggac gacgtcgatc tccagcggct tgtcgacgcc
     4081 ctggcggatg atggtgagct tgatgacgga accgggttta ccccgcatga gcttgaccgc
     4141 gtcgttcagg ctgaggcctt tgaccggggt gtcgtccagc ttgatgatca gatcgccggt
     4201 gagtacgccg gctttttgcg cgggggtgtc gtcgatcggg gtgatgacct tgacgaaccc
     4261 gtcttccatg cccacttcaa tgcccaggcc gccgaattcg ccggtggtgc tgatctgtag
     4321 gtcctggtac gcctcgggtt ccagatacgc cgaatgcgga tcgagttcgg tgagcatgcc
     4381 cttgatggcg ttttccagca gggtcttgtc gtccacttct tctacatagg cctgcttgat
     4441 gcggtcgaac acgtcggcga aggtgcgcag ttcgttcagc ggcagcaaac cctgttgcgg
     4501 ggcaggcgcg gctgtggcgg gttgggccgg ggcagtt
//
LOCUS       DASUEG010000183         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1177686, whole genome shotgun sequence.
ACCESSION   DASUEG010000183 DASUEG010000000
VERSION     DASUEG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177686"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFX11_03500"
     CDS             <1..743
                     /locus_tag="VFX11_03500"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4937712.1"
                     /translation="AMDAALSCGVERCVFTSTMGTLGINPAGPVTEDTEFNWVDRAPP
                     YILARLQAERHFLRYCRERGLPGVALCVANTYGPEDYQPTPHGKMLWDVARGKVRFTI
                     DAGAPTVDIRDVAQAALLAEQHGKSGDRYIIANEFISNRDFYNMAAAEGGSQPVHVVA
                     RPLAYGIAWTAERLLKLFGQKDYLVSTDAVFLSNVFQEMDNSKARRELRWSPRPIRET
                     VRDAVAWYAQRENANRRDSPAKKTPAGT"
     gene            complement(774..1751)
                     /locus_tag="VFX11_03505"
     CDS             complement(774..1751)
                     /locus_tag="VFX11_03505"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX4937713.1"
                     /translation="MSFKTRITELLGIEYPIVQGGMMWVGRAELAAAVSNAGGLGILT
                     ALTQPSPDELRREIDRCRSMTDKPFGVNLTILPSVNPPPYAAYRKAIIDGGIKVVETA
                     GHNPQEHVEDFKAHGIRVIHKCTAVRHALSAERMGVDAISIDGFECAGHPGEDDIPGL
                     ILIPAAANKVKIPMLASGGFADGRGLVAALALGADGINMGTRFCATVEAPIHQRVKQF
                     LVDNDERATNLIFRSLRNTGRVGKNSVSDEVVKILKNPEAKFEHVSHLVKGADGRKAL
                     ESGDINAGLIWAGQVQGLINDIPTVAELMHRIIGEARDIIQSRLNPVLG"
     gene            complement(1789..2556)
                     /locus_tag="VFX11_03510"
     CDS             complement(1789..2556)
                     /locus_tag="VFX11_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX4937714.1"
                     /translation="MMKDVKGLAAIVTGGASGLGAATAEMLASAGAKVTIFDMNEEAG
                     QAQAKKINGTFIKVNVTDEDNVREAIATAEKQNGIARILVNCAGIATGAKTVGKENTP
                     HPLGQFRKTIEINLIGTFTVIAHFAARLSTAEPIGEERGVIVNTASVAAFDGQIGQAA
                     YSASKGGVVGMTLPIARDLAQFGIRVMAIAPGIFYTPMMAGMPQNVQDALGKQVPFPS
                     RLGQPSEYALMVHSIIANPMLNGEVIRLDGAIRMPPR"
BASE COUNT          539 a          743 c          777 g          620 t
ORIGIN      
        1 atgccatgga cgctgccttg tcctgtggtg tcgagcgatg tgtttttacc agtaccatgg
       61 gtactctggg aatcaatcct gccggaccgg ttacggaaga cactgaattc aactgggttg
      121 atcgggcgcc gccctacatc cttgcccggt tgcaggcgga gaggcatttt cttcgttact
      181 gtcgtgaaag gggattgccg ggtgtggctt tgtgcgtggc caatacctac ggccctgagg
      241 attaccagcc aacgccgcac ggaaaaatgc tgtgggatgt tgcgcggggc aaagttcgct
      301 tcaccattga tgcgggtgcg cctaccgttg atattcggga cgttgcgcag gctgcgttgc
      361 tggcggagca gcatggcaaa tctggtgatc gttacattat tgcaaatgaa ttcatcagca
      421 atcgtgattt ctataacatg gccgcagctg agggaggcag tcagccggtg catgtggtgg
      481 cccgcccttt ggcatacggt atcgcctgga cggccgagag attgctgaaa ctgtttggac
      541 aaaaagacta tctagtgagt actgacgctg tttttctctc caatgtcttt caggagatgg
      601 ataacagcaa ggcccgcagg gaattgcgtt ggagtccgcg ccccatcagg gaaacggtga
      661 gggatgcagt ggcctggtac gcgcaaaggg aaaatgcaaa tcgacgcgac agtcccgcca
      721 aaaaaacgcc tgctggtact tgaggaaaag cagtcagcag gcgcaacgtc aaactacccc
      781 agtaccggat tcaggcgact ctgaatgatg tccctggctt cgccaataat ccgatgcatc
      841 aattctgcaa cggtaggaat atcattgatc aggccctgta cctgacctgc ccagatcaga
      901 cctgcattga tgtctccgct ttcgagcgct ttcctgccgt cggcgccctt caccagatgc
      961 gatacatgtt caaattttgc ttccggattc ttgaggattt tcaccacctc atccgaaacg
     1021 ctgttcttgc cgacgcggcc ggtgttgcgc agtgagcgga aaatcagatt ggtggcgcgt
     1081 tcatcgttat ccaccaggaa ttgttttacg cgctggtgga tgggtgcttc gacggtggcg
     1141 cagaagcggg tgcccatatt gattccgtct gcgcccagcg ccagtgcggc taccagaccc
     1201 cggccatcgg caaagccgcc ggaagccagc atggggatct tgaccttgtt ggcagccgcg
     1261 ggaatcagga tcaaccctgg aatgtcatct tcacccgggt ggcctgcgca ctcaaaacca
     1321 tcgatggaaa tggcatccac gcccatgcgc tcggcggaca aggcatgacg aaccgcggtg
     1381 catttgtgaa tcaccctgat gccgtgcgcc ttgaagtctt ccacatgttc ctgcggattg
     1441 tggccggcgg tttccaccac cttgatgcca ccgtcgatga tggccttgcg ataggccgcg
     1501 taaggagggg gattcaccga aggcagtatg gtcaggttga cgccgaaagg tttgtcggtc
     1561 atggaccggc aacgatcgat ttcccggcgc aattcatcgg gcgagggttg tgtcagtgcg
     1621 gtcagaatgc ccaggccgcc ggcattggac acagcggccg ccagttctgc gcgcccgacc
     1681 cacatcatgc cgccctgtac gatggggtat tcgatgccca gcaattcggt aatgcgtgtt
     1741 ttgaatgaca tgtctttttc tcacgtaaat ttgtctgcga cctgtgtctc agcgtggcgg
     1801 catgcggatc gcgccgtcca ggcgaatgac ttcgccattg agcatgggat tggcaatgat
     1861 gctgtgtacc atcagcgcgt attccgacgg ctgccccaga cggctgggga agggcacctg
     1921 tttgcccagt gcgtcctgta cgttctgcgg catgcccgcc atcatcggtg tgtagaaaat
     1981 gccgggagcg atagccatta cgcgaattcc gaactgcgcg agatcgcgtg cgatgggcaa
     2041 tgtcatgccg accacgccgc ctttggacgc cgagtacgca gcctgaccga tctgaccgtc
     2101 gaaagcggca acgctggcgg tattcacaat gacgccacgt tcttcaccaa tgggttcggc
     2161 ggtcgacaac cgtgctgcaa agtgggcgat gaccgtgaag gtgccgatca ggttgatctc
     2221 aatggtcttg cggaactggc ccagcgggtg cggcgtgttt tccttgccta ccgttttggc
     2281 gccggttgca atgccggcac agttcaccag aatgcgcgcg ataccatttt gcttctctgc
     2341 agtggcaatg gcttcccgca cattgtcttc atcggtgaca ttcaccttga tgaaggtgcc
     2401 gttgattttt ttggcctggg cctggccggc ttcctcgttc atatcaaaaa tagtcacttt
     2461 ggcgccggcg ctggcgagca tttctgctgt cgctgcgccc agaccagagg caccgcccgt
     2521 gacaatggcg gctaatccct tgacgtcttt catcatccaa cctcgttcaa atacggtttt
     2581 gttcagatca gttcgacagc catggcagtg gcttcaccac cacccagaca aatgccagct
     2641 acgccccgtt tcaagccacg gtttttcagc gcggccaac
//
LOCUS       DASUEG010000184         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_359675, whole genome shotgun sequence.
ACCESSION   DASUEG010000184 DASUEG010000000
VERSION     DASUEG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359675"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFX11_03515"
     CDS             <1..723
                     /locus_tag="VFX11_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4937715.1"
                     /translation="YRQHMIDSGMNPDAKAFNMFFTKSTASITGPNGKIIKPRHVTLL
                     DYEIELTLVLGKRTDSTVTVTEGNLHEYVAGICIGNDISARDVQIPQMQFHKGKSYRT
                     FCPLGPVLCLLEPEEMHYLHDLQLTLTVNAGIRQQDSTANLVFKPAESLTEYSQIQNF
                     EPGDVLMTGTPAGCALGLPSPTAVRLSALLPEAKKWELFLKSQRSRKQYLKAGDKVES
                     TIVSRDGKINLGRQSHVIENEK"
     gene            720..2687
                     /locus_tag="VFX11_03520"
     CDS             720..2687
                     /locus_tag="VFX11_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX4937716.1"
                     /translation="MKQGQGISTISDVLAIEAAGIPPLPASTYEMLCAGANLDPDAIA
                     LSFFLQAKEHRNPETWSYRQLLDNINRTANFFHSLGATKDTVIAFILPNLPETHFVIW
                     GGQAAGIVAAINPLLEPTAIAELLNAAGATILVTLAPFPGTDLWPKVSKVLPKVASLR
                     HLVLVDLADRVRGPKHFLARLAQKKETVQLHGLQGIRKALPRGIRAHDFGSAIRHQNR
                     HGLDSGRCIDPGDYSSFFCTGGTTGTPKIAMRTHRNEVANAWSVAQTIGSGMSNRKNV
                     FCGLPLFHVNGVLVTGLIPFWKGAHVILGTPQGYRGEGVIPGFWEIVAHHQINFFSGV
                     PTLYAGLLQVPIGHHRIDCLEYGLCGAAPMPVEVFRRFQDSTGLKILEGYGLTEGTCV
                     SSVNPSLGDRRLGSIGIRIPGQQMKTVVLDAEGRYERDCRIDEAGVIAISGPNVFSGY
                     RIPEQNKGLWIEAGDGQRWLNTGDLGRQDKDGYFWLTGRKKELIIRGGHNIDPASIEE
                     PLHRHPAIQLAAAVGRPDAHAGEIPVAYVQLKPGAVINADELLAFAQSEIPERAAWPK
                     QIRIVDQIPLTAVGKIYKPTLKHREIKDALSEALKKHNIEVKHIHVTEDKTLGTRVQV
                     ALAEDTCESAKRVLGQFPFEVRFEALEDISG"
     gene            2680..>3739
                     /locus_tag="VFX11_03525"
     CDS             2680..>3739
                     /locus_tag="VFX11_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4937717.1"
                     /translation="MDNVSVTLAHRCFLAALAFSLPCVADASAVTYAISPIEQALTLA
                     QVRTQSRVETLLVTAQTDTTITAINLSRLLQIHVDDPLELVTRVGADRVRELAGSPAG
                     NYSVSDLVIPGGLASEQLAAGANYAAHGEEGGLSHTFLFPKFALPTAHSSSISVHQNQ
                     LLDYEVEICARFDRNIQSVTDVNNANVGLFLCGDFTDRATLMREIDKDNWESGVGYTD
                     AKSGAGLFPIGAFTVVPLDWASFIDGIDLQLSVNGEIRQQDSAKKLLLKLDDILALAF
                     AGHTSMRWTFRGQDIPLLRDGILYRGQSILTGTPEGVVFRPPGTLFIISSVLKWLATG
                     SFMNSNVADYVIESYIADR"
BASE COUNT          873 a         1060 c         1027 g          779 t
ORIGIN      
        1 tatcgccagc acatgatcga ctccggcatg aatccggatg ccaaagcctt caatatgttt
       61 ttcaccaaat ccactgcgtc catcaccggg cccaacggaa agatcatcaa gcctcgtcat
      121 gtcacgctgc tcgactatga aatcgagctt acgctggtac tgggcaagcg caccgacagc
      181 accgtcacgg ttaccgaagg caatctgcac gaatacgtgg caggcatctg cataggcaac
      241 gatatttccg cccgcgacgt gcaaattccg cagatgcagt ttcacaaggg caagagttat
      301 cgcacgttct gcccgctggg gccggtactg tgtctgctgg agccggaaga aatgcattat
      361 ctccatgatt tgcagctgac tctgaccgtc aacgccggta tccgacagca ggattcaacc
      421 gccaacctgg ttttcaaacc ggcggaatct ctcaccgaat actcgcaaat ccaaaatttc
      481 gaaccgggcg atgtgttgat gaccggaacc ccggctggat gtgcgctggg tctgccgtca
      541 cctacggccg tccgtctgtc tgcactgctg cccgaagcca aaaagtggga gctttttctc
      601 aaatcccaac gcagccggaa acagtacctg aaagccggtg acaaagtgga aagcaccatc
      661 gtcagccggg acggcaaaat caatctgggc cggcaaagcc atgtcattga gaacgaaaaa
      721 tgaaacaggg tcagggcatt tccaccatca gcgatgtgct ggccattgaa gccgcgggga
      781 ttccgccact ccctgccagt acctatgaaa tgttatgtgc gggcgccaac cttgatccgg
      841 atgccattgc actctctttc ttcctgcagg caaaagaaca ccgcaatccg gaaacctgga
      901 gctaccggca attgctggac aacatcaaca ggacagcaaa cttctttcat tcactgggcg
      961 ccacgaagga cacggtcatc gcgtttattc tgcccaacct gcccgagacg cacttcgtta
     1021 tctggggtgg acaggccgcc ggcatcgtcg ctgccatcaa cccgctgctg gagccaacgg
     1081 ccattgcgga gctgctgaat gcagcaggcg ccaccatact ggtcaccctc gcgcctttcc
     1141 cgggcaccga tctgtggcca aaagtgagca aggtcctacc gaaggttgcc agcctgcgcc
     1201 atctggttct ggttgatctg gctgaccggg tgcgggggcc aaaacatttt ctggcgcgac
     1261 tggcgcagaa aaaggaaacc gtccagctgc atggtctgca gggaatcaga aaagcgctgc
     1321 cccgcgggat cagggcgcat gactttggca gcgccatcag gcatcagaac aggcatgggc
     1381 tggattcggg aagatgcatt gatcctggcg attattcctc attcttctgc accggcggca
     1441 caacgggcac acccaaaatc gccatgcgga cccatcgcaa tgaagtagcg aacgcctgga
     1501 gcgtggcgca aacaatcggc tccggcatga gtaaccgcaa gaacgttttc tgtggactgc
     1561 cgctttttca tgtcaatggg gttctggtga cagggctgat tcccttctgg aagggcgccc
     1621 atgtcattct gggcactccc cagggctatc gtggcgaggg cgtcataccc ggattctggg
     1681 aaatcgtggc gcatcaccag atcaatttct tcagcggcgt gcccactttg tacgcgggct
     1741 tgttgcaggt acccatcggg catcacagaa tcgattgcct tgaatacggt ttatgtggtg
     1801 cagccccaat gcctgtcgaa gtatttcgcc ggttccagga ttcaacgggc ctcaaaatac
     1861 tggaaggcta tggcctcacc gaaggcactt gcgtgagcag tgtcaatcca tcccttggcg
     1921 accgcaggct aggctcgatc gggatcagaa tcccaggcca gcagatgaaa accgttgttc
     1981 tcgacgcaga ggggcgctac gaacgggatt gcagaatcga tgaagcgggt gtcatcgcca
     2041 tttcgggccc caacgtattt tccggctaca ggataccgga gcaaaacaag ggattgtgga
     2101 tcgaagccgg agacggccag cgctggctca acaccggcga tctgggcagg caggacaagg
     2161 atggctattt ctggctaacc ggccgcaaaa aggaattgat cattcgcggt gggcacaaca
     2221 tcgatcccgc atccattgaa gagcctttgc accggcatcc cgcgattcaa ctggcagcag
     2281 cagtgggtcg accggatgcg catgcaggtg aaattccggt tgcctatgtt cagttgaagc
     2341 caggcgcagt catcaacgcg gacgaactcc tggcgtttgc gcaatctgaa atccccgaac
     2401 gtgccgcctg gccgaagcag atcaggattg tggaccagat tccgctgacg gccgttggca
     2461 agatatacaa gcccacactc aagcacaggg aaataaaaga tgcgctcagt gaagcactca
     2521 aaaagcacaa cattgaggta aaacacatac atgtcacgga agacaaaacc ctgggaacca
     2581 gggtccaggt ggcgctggca gaagatacct gcgaatcagc caaacgtgtt ctgggccagt
     2641 ttccatttga ggtccggttt gaagcgctgg aggacatcag tggataatgt cagcgtgaca
     2701 ttggcacacc ggtgcttcct tgccgcgctt gcattttcac taccgtgcgt ggccgacgct
     2761 tccgctgtaa cgtatgcaat ttcgcccatt gaacaggcat tgacgctggc acaggtgaga
     2821 acgcaatcgc gggttgaaac gttgctggtt acggcgcaaa cggacaccac catcaccgcc
     2881 atcaacctga gccggctact tcagatccac gttgacgatc cactggagct ggtcacccgt
     2941 gtcggcgctg accgcgtgcg cgaactggcg ggttcaccgg caggcaacta ttcggtgtcc
     3001 gatcttgtca taccaggtgg actggcctct gaacagctgg ccgctggtgc caattatgct
     3061 gcccacggag aagaaggcgg gctcagccac acgtttttat tccccaagtt cgctcttcca
     3121 accgcgcaca gctcttccat ttctgttcac caaaatcaat tattggatta cgaagtcgaa
     3181 atctgcgcgc ggttcgatcg caacatccag tcagtgaccg acgttaacaa cgcgaacgtg
     3241 ggattgtttc tgtgcggtga ttttacggac agggccaccc tgatgcgcga aattgacaag
     3301 gacaactggg agtcaggtgt tggctatacg gatgcaaaaa gtggcgcggg gctttttccc
     3361 attggcgcgt tcaccgtcgt acctctcgac tgggcatcgt tcatcgacgg catagacttg
     3421 cagctatccg tcaatggtga aatccgtcaa caggacagtg cgaagaaact gctgctcaag
     3481 ctggacgaca ttctcgccct ggcttttgcc ggacatactt caatgcgctg gacttttcgc
     3541 ggtcaggata ttccgctgct ccgggatggc atcctgtacc gtggacaatc aattctcacg
     3601 ggcactcccg agggcgtggt tttccgccca cccggaaccc tcttcatcat ttccagcgta
     3661 ttgaagtggt tggcaacggg gagtttcatg aacagcaacg tcgcggacta cgtgattgaa
     3721 agctacatag cggatcgac
//
LOCUS       DASUEG010000185         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1221607, whole genome shotgun sequence.
ACCESSION   DASUEG010000185 DASUEG010000000
VERSION     DASUEG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221607"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1079
                     /locus_tag="VFX11_03530"
     CDS             258..1079
                     /locus_tag="VFX11_03530"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX4937718.1"
                     /translation="MGRKILVPVLIVIAGLIAIAWMLRQQPATAPTPGSLAPPVAAPA
                     PDKPQSHPVIRDEAAGSVETTPAIATAREEAGSAQIPVIAIPPQLDDSDAYVLQAATE
                     LNPQVTGWLIPQEQLRKWVMLVNLVAEGKFPVKERPLSYDLQPFRARKENDRFWLDAA
                     SFSRKDVLVSALTTIPPSRAADYFHAWEPLLQRAHDELGIGGQFRDRLQLAITRVNSV
                     QPLQGDVELKQSIISYQYLDPILEQSSALEKLLWRLGPDNSARLQAYLKQLAPLL"
     gene            complement(1113..2699)
                     /locus_tag="VFX11_03535"
     CDS             complement(1113..2699)
                     /locus_tag="VFX11_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937719.1"
                     /translation="MPQFEPGLVLGALLIFIYATERFNTPRTIRASTTAGRYYSAATI
                     YLLIYLLTFYLFSKYPHLLKVLINDADLQKQMSAMDSSNTPIFVAMIFSLLVPKVPLI
                     SELDNRLRIFLHRLASIPYEAIRMSKEVQGMRFEVPDAVAEELRREMEERNFPVQVDS
                     VDNADPIVRNWMKIAALVIQLRHWEQANPFAPFMQERSGQLQRIHERYLRLCTAAINA
                     YALCQQAKSQPEVPALQDAALRFCRNLRADEKSIYSELCDFISQAILSNCYRAQTRRQ
                     ALAQLGFQSKESIIYEGLSIHQGVTLAGLLLMLLLTSFILFSQSVMEIELLLLRATMI
                     VSIYSAAVFFAVYPKAQWPHFQHREGQFYPVASYLVSGVLAMVASALINLTFRTLINI
                     SDSKNDGVAMAMQEAWQRFSTLAYPWLCMSFVATMVLAFLIDWTIPARLNERWQRWCK
                     AVVLMVTLMGATVLVHQWLQELYGALQKPFPVELHSLLRNSAIIGLVLGYFVPAWFRR
                     SSLQRRTRVIEAPELAPALT"
BASE COUNT          656 a          898 c          825 g          537 t
ORIGIN      
        1 atcgcgcgct ccagccctat cgctgcatgg gtaaaggatt ccggcgccag gtacagggcg
       61 gcagtggcgg tggcacccac cagcagaaca aaaacgatca ggtatcgaag tacgcgcacg
      121 ggggcctcac gggatcaggg caggaatgaa cggcccatta tatacagtgc gcgtccggtc
      181 tcatatgtga acgcaaggtt tgttagaatg gttcgcggcg catcgccaca ctttgaattt
      241 gagcaggggg aatcatcatg ggcaggaaaa ttctggtacc ggtgcttatt gtcattgccg
      301 gcctgatcgc cattgcctgg atgctgcgcc agcaaccggc caccgctccc acacccggca
      361 gtcttgcgcc gccggttgcc gctcccgcac cggataaacc tcaatcccat ccggttatcc
      421 gcgatgaagc cgcaggcagc gtggaaacga cgcccgccat cgccactgcc cgcgaagagg
      481 ccggcagtgc gcagatcccc gtcatcgcca taccaccgca actggatgac agtgacgcct
      541 acgtcttgca ggctgccacc gaactcaacc cgcaggtcac cggctggctg atcccgcagg
      601 agcagctgcg caaatgggtg atgctggtga atctggtggc agaaggcaaa tttccggtga
      661 aagagcggcc tttgtcctac gacctgcagc cgtttcgcgc gcgcaaggaa aatgaccgct
      721 tctggctgga tgccgccagc ttcagccgca aggacgtcct ggtgtccgcg ctgactacca
      781 tcccgccatc ccgcgcggcg gattatttcc acgcctggga accgctgctg caacgggcac
      841 acgacgagct gggcatcggc ggccagttcc gcgatcgcct gcagctggcc atcacccgtg
      901 tcaacagcgt gcagccgttg cagggtgacg ttgaactgaa acagtcgatc atctcctacc
      961 agtatctgga tccaatcctg gagcagtcca gcgcgctgga gaaactgctg tggcggctgg
     1021 gccccgacaa cagcgcccgc ctgcaggcct acctgaagca gctggcgccg ctgctgtaat
     1081 ccggcccgcc atcacccgat tccggtgcag gttcacgtca gcgcgggggc cagctcgggc
     1141 gcttcgatca cccgcgtccg ccgctgcaga ctggagcgcc ggaaccaggc cggcacgaaa
     1201 taccccagca ccagaccgat gatcgcgctg ttgcgcagca agctgtgcaa ttcaaccgga
     1261 aacggcttct gcaacgcgcc gtacaattcc tgcaaccact gatgcaccag caccgtagcg
     1321 cccatcagcg tcaccatcag caccactgcc ttgcaccagc gctgccagcg ctcattcagc
     1381 cgggccggga tcgtccagtc gatcaggaac gccagcacca tcgtggcgac aaacgacatg
     1441 cacagccacg gataggccag cgttgaaaag cgctgccagg cttcctgcat tgccatcgct
     1501 actccgtcat tcttgctgtc actgatattg atcagggttc tgaatgtcag attgatcagt
     1561 gcgctggcca ccattgccag cactccggac accaggtaac tggccaccgg ataaaactgc
     1621 ccttcccgat gctggaaatg cggccactgc gccttgggat agaccgcaaa aaaaacggca
     1681 gcggaataaa tggacacaat catggtggca cgcagcaaca gcagctcgat ctccatgaca
     1741 ctttgcgaga acaggatgaa gctggtgagc agcagcatca gcagcaaacc cgccagcgta
     1801 acaccctgat ggatggaaag gccttcgtag atgatgctct ctttcgactg gaacccgagc
     1861 tgggccagcg cctggcgccg ggtctgcgcc cggtagcagt tggacagaat cgcctggctg
     1921 atgaaatcgc acaactcgct gtaaatggat ttttcatccg cacgcaggtt tcggcagaag
     1981 cgcaacgcgg catcctgcag ggccggcact tccggctggg atttggcctg ctggcacaac
     2041 gcatacgcat tgatggcagc cgtgcacaac cgcaggtaac gttcatggat gcgctgcaac
     2101 tggccgctgc gttcctgcat gaaaggcgca aacggattgg cctgctccca atgccgcagc
     2161 tgaatgacca gcgccgcaat tttcatccag ttgcgcacga tcggatcggc attgtccacc
     2221 gagtccacct gcaccggaaa attgcgctct tccatttcgc ggcgcagctc ctctgccacc
     2281 gcatccggaa cctcgaagcg catcccctgc acttccttgc tcatgcgaat ggcctcgtag
     2341 ggaatggagg ccagccggtg cagaaaaatg cgcagccggt tatccagttc ggagatcagc
     2401 ggcaccttgg gcaccagcag cgaaaaaatc atggccacga aaatgggcgt gttgctgctg
     2461 tccattgcac tcatctgttt ctgcaggtca gcgtcattga tgagcacttt cagcaggtgg
     2521 ggatatttgc tgaacaggta aaacgtcagc agatagatca gcaggtaaat ggtggcggca
     2581 gaatagtaac ggcccgccgt ggtggacgcg cggatggtgc gcggtgtatt gaagcgttct
     2641 gtggcataga taaaaatcaa caacgcaccc agaaccagcc ctggttcaaa ttgcggcata
     2701 tacatgccct ccggaatagc gattcaccgg caacgaaaac cggttcacgt ccgcagtaaa
     2761 aatgagccgg gacaaacctg tcaacgcact ttccggcctt cgtgaaaagt gcggcaatcc
     2821 cgtcgccaaa agtggcaacg ggttcattga acgccgaaat aatcagccgt ttttagcgcg
     2881 tgcagaaata acgcttatac gctttggcta tgacga
//
LOCUS       DASUEG010000186         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1663203, whole genome shotgun sequence.
ACCESSION   DASUEG010000186 DASUEG010000000
VERSION     DASUEG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663203"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2669)
                     /locus_tag="VFX11_03540"
     CDS             complement(<1..>2669)
                     /locus_tag="VFX11_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937720.1"
                     /translation="GRSGRLKIDAERLSGGLDGLAKLLDSSSAHGGVDLRLRGSEQQV
                     LSAGQVLKADDIALAFDQAGVEIHGQLDASGKRGGSIDVQANGDVFIGAGAGLLARGS
                     ENRGGDVRVSSHGGALSFAAGSTVDVSGGEGGDVVFRADIDDTRGVNIGDAGFAGSII
                     GARRARLEVHDTPYLDNGTFDQADADALRARAQAIQATADSIRARLLVDEQVDIVSSL
                     EVVSQGAYNWSQEVNFDEWQSYTDTGTLADGSVLAGTLTVKATGNLSMLGGLSDGFNT
                     EGLLSTQDSWSYRLVAGADLAAANALHTAVSQANFVVGKDTKIRTGNGRIDIASANDF
                     SLTNRNSVVYSAGRNTGLVLDPNDALVFGIAGEKVVAAEGGHVSITAGHDLKGHAPAK
                     GNGVRVQAWTDWLQDNGAVAEEVLIDPETGEPLIDAETGGYYYMRVQEDAGRFVNYAA
                     FAQGIAAIGGGNLSLVAGQDIVRLDASLPTTIAVSSQTGLRTETGAGQLRVHAAGDVI
                     SSHFLIGEGEGSVTALGSIRAERTLADGRVAAGYTALAGGDFRYSARKDVTLLGAFDL
                     SSVNHLFADGMDSSAKYYFSMDGDTAFSAMSIAGNLDFNTTLSAAQRNLFDLQGENLF
                     ASGPYSQDKIVPADFTLAALSGKTSVENLLSFAQADSTLVLWASDSLFLKSGVTLMDV
                     PLAELPTLENPESPKGGGFDPRQGVQVSSLSALENEESNILLVATDGSVSMPSVFRSA
                     LPVDVFAAQDIDRINMVATHYDETDVSRFTAGRDIKLVSGAERISVAGPGRVDVMAGR
                     NINLGSSKGIETVGATTNRQLQGREGADLFVFAGVRSSDLQYDSFIVPLLDNRSSMNS
                     FATQVQSYVALVTTEKLSERDASERFRSLTLAQ"
BASE COUNT          626 a          856 c          649 g          538 t
ORIGIN      
        1 ctgtgccagc gtcaagcttc tgaatcgctc ggaggcatca cgttccgaca acttttcagt
       61 cgtgactaaa gcaacataag attgaacctg cgtggcaaag ctgttcatcg aactgcgatt
      121 gtccaacaac ggcacgataa aactgtcata ttgcaaatcg ctcgaacgaa cgccggcgaa
      181 tacaaataga tcagcccctt cacggccctg caattgtctg ttggtcgttg ccccaacagt
      241 ctcgatcccc ttggacgaac ccagattgat attgcggccc gccatgacgt ctacccgtcc
      301 aggtcccgca acactgatgc gctccgcgcc agaaacgagt ttgatatcac gccctgcggt
      361 aaagcgcgat acatctgtct catcgtaatg ggtagcaacc atgttgatgc ggtcgatgtc
      421 ctgagcggca aatacatcca ccggcaatgc ggaacggaac acggatggca tcgaaacact
      481 gccgtccgtc gccaccagca aaatattcga ctcttcattt tccagtgcag ataacgagct
      541 cacctgcaca ccctggcgcg ggtcaaaacc gccaccctta ggcgattccg gattttccag
      601 tgtcggtaat tcggcaaggg gaacatccat caaggtcacg ccggatttaa gaaacagcga
      661 atcgctcgcc cacagcacaa gagtcgaatc cgcttgcgca aaactgagca gattttcgac
      721 tgaggttttt ccacttaagg ctgccagtgt aaaatctgca ggcacaatct tgtcctgact
      781 gtaagggcca ctggcgaaca ggttttcgcc ttgcagatcg aacaagttgc gctgggcggc
      841 ggacagtgtc gtattgaaat ccagattccc ggcgatactc atggctgaga acgcagtatc
      901 gccatccatc gagaaataat atttcgccga gctatccatg ccgtcagcaa aaagatgatt
      961 cacagagctc aaatcgaaag cacccaacaa ggtcacatcc ttacgcgccg agtaacggaa
     1021 gtcgccaccc gccagcgcgg tgtaacccgc tgccacacga ccatccgcca aggtccgttc
     1081 tgcgcgaata ctgcccaggg cagtgacact gccctcacct tcaccaatca aaaaatgact
     1141 gctgatgacg tcgcccgccg catgcacccg cagttggccc gcacctgttt cagtgcgaag
     1201 gcccgtttgg gaactcacgg caatggtcgt gggtaatgac gcgtccagac gcacaatgtc
     1261 ctgaccggcc acaagcgaca gattgccccc accgattgca gcaattcctt gtgcgaaggc
     1321 cgcataattg acaaaacggc cggcatcttc ttgcacgcgc atgtagtagt agccacccgt
     1381 ttcagcatcg atcaatggct cgcctgtctc aggatcgatc agcacctctt cggccaccgc
     1441 accgttatcc tgcaaccaat cggtccaggc ctgtacgcgt accccgttgc ccttggctgg
     1501 cgcatgccct ttgagatcat gcccggcagt gatactgaca tgaccacctt ccgctgccac
     1561 gactttctca cccgcaatgc caaagaccag cgcatcgttc gggtcaagca ccagaccggt
     1621 attacggccc gcgctgtaca ccacagaatt ccggttggtc agcgaaaaat cattggcaga
     1681 agcaatgtcg atacggccgt tacccgtgcg tatcttcgta tccttgccca ccacaaaatt
     1741 tgcctgtgat actgcggtat gcaaggcgtt ggcggcagca agatcggcac ctgccaccag
     1801 gcgatagctc cagctgtcct gggtgctcaa caagccttct gtgttgaagc cgtccgaaag
     1861 accgcccaac atagagaggt tacctgtcgc tttcaccgtc agcgtgccag ccaacacact
     1921 gccatcggcc agcgttcccg tgtcggtgta gctttgccac tcatcgaaat tcacttcctg
     1981 tgaccagtta taggcgcctt gcgacaccac ctccagcgac gaaacgatat cgacctgctc
     2041 atccactaac aaacgggcac gaatcgaatc ggcggtggcc tgaatggcct gcgcccgcgc
     2101 ccgcagtgca tcagcatccg cttgatcaaa agttccattg tcgagatagg gggtgtcgtg
     2161 cacttccagg cgggcacgac gcgccccgat aattgacccg gcaaatccgg catcgccgat
     2221 attcacaccg cgcgtgtcat cgatatcagc acggaagacc acatcgccgc cttcaccgcc
     2281 gctgacatcc accgttgaac ctgcggcaaa cgacagtgcg ccaccatggg acgacacccg
     2341 cacatcgcca ccacggtttt cgctgccacg cgcaagtaaa ccggcgccgg cgccgatgaa
     2401 gacatcaccg ttcgcctgta cgtcaatgct gcccccgcgc ttgcctgatg catcgagttg
     2461 tccatggatt tcaacaccgg cctgatcaaa ggcaagtgca atgtcgtcgg ccttcaatac
     2521 ctggccagcg ctcagtacct gctgttcgct accacgcaag cgcaggtcca caccaccgtg
     2581 ggcgctgctg ctgtctaaaa gcttcgccag gccatccagc ccacccgaca accgttcggc
     2641 atcgattttt aaacgccccg accgtcctt
//
LOCUS       DASUEG010000187         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1344408, whole genome shotgun sequence.
ACCESSION   DASUEG010000187 DASUEG010000000
VERSION     DASUEG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344408"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1076
                     /locus_tag="VFX11_03545"
     CDS             282..1076
                     /locus_tag="VFX11_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937721.1"
                     /translation="MTSSPKVAQSPWFQVPRPVDAPRLRLFCFSYAGGNASTYRDWHR
                     LLPADVEVCSVQLPGRGSRFKERAHTTLNSLLDALQTAIQPYVGVPYAFFGHSMGAQV
                     AFELARRLRDSGARQPACLIVSGRRGPQRPPRNKPIHTLPEPQFRDEIRRLNGTPEEA
                     LNNPELMDLVSPILRADCQVVETWEYRPSEPIDVPVLALGGVKDKQVSLDDLEDWRSV
                     TKGPFSLELFSGDHFFINQVTESLLNTVSQALNCVLGRAEGLLSRR"
     gene            1307..>2542
                     /locus_tag="VFX11_03550"
     CDS             1307..>2542
                     /locus_tag="VFX11_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4937722.1"
                     /translation="MKKLLKIGLPVVAVVAALAVYFFHFANKTEVASQKPEVRIPLVN
                     IAPVRAQSMNIPLFTRGTVTPSTQIHLVSEVSGQIVEISPNFSAGGFFKRGEVLVRVD
                     TLEYDVMIKRAEAAKAQAYQTFLQAKAERKARSMGRSVQDNTLASFEVQVRQAEAHYL
                     ATSSELEAMKKQRERTTVRAPFDGRVLTAGLNIGQFLRPGAQMGVIYAVDVAEVRLPL
                     SDRQLGLVDVPGRMSDQETLAMPEVLFTEEFAGKTFTWKGQVVRTEGGVDERNRLLYV
                     VAHVPDPYGIDAGQPGRPELVAGSFVEAKIAGRKFERVFAIPRKALRNGNEVWVVTDD
                     SRLSKREIGILHKEKDTVYINTGIDDGEQVVLNQLDIAVEGMRVRTQLQENPDQVESE
                     VVAGDNPFAGGRQVAPAITA"
BASE COUNT          516 a          733 c          728 g          565 t
ORIGIN      
        1 ctgtcggccc agaccgtccg tccagcgacg aacggctgac tgtaagttgt gtaacttgtt
       61 gttttcaaac tgttaagttt tcacatttct cgtatcccgg gtttgaccgg ggcaggtgta
      121 tcgcttttaa ttcttggtag acagtggaat aactgcggta tacttttcct attcttgcac
      181 cgttgtacta ctttggtgct cagcagtcac attggggttg cccgctatcc ggcgacggtt
      241 caggcaaaat aacaagaaaa cggtggcagt aagcaatggt catgacatcc agtcccaagg
      301 ttgcccagag tccctggttc caggttcctc gtcctgtcga tgcgccccgg ttgcggttgt
      361 tctgtttttc ctacgccggc ggcaatgcat ccacctatcg cgactggcac cgtttgctgc
      421 ccgccgacgt tgaagtctgt tccgtccagt tacccggccg tggctcccgt ttcaaggagc
      481 gggcccatac caccctgaac agcctgcttg atgccctgca gacagccatt cagccttatg
      541 tcggtgttcc ctacgcattt ttcggccaca gcatgggcgc ccaggtggcg tttgaactgg
      601 cccggcgtct gcgtgattcc ggcgcacgcc agccagcctg tctgatcgtg tccggcagac
      661 ggggcccgca gcgtccaccg cgtaacaaac ctattcacac cttgccggaa ccccaattcc
      721 gcgatgaaat ccgtcgcctg aatggcactc cggaagaagc gctgaacaat cccgaactga
      781 tggatctcgt cagcccgatc ctgcgcgccg attgccaggt tgtggaaacc tgggaatacc
      841 gcccgtccga gcccatcgac gtgccagtac tggcattggg tggagtcaag gacaaacagg
      901 tgtccctgga tgatctggaa gactggcgtt ccgtgacaaa aggaccgttc agccttgagt
      961 tgttctccgg ggaccacttc ttcatcaacc aggtgaccga atccctgctc aataccgtca
     1021 gccaggccct gaactgtgtg ttggggcggg ccgagggtct gctttccagg cgttgattct
     1081 gtcgaaatct ccggctcccc atcgtcgtca ccattcgcca ttggaagtcg ctgctgctac
     1141 agtgacttcc aagtgcaaat tcttggttca accaattaat ttttgggatc aattcagcta
     1201 atatgccgga tcgccgcatg tttacggtac cctgcatgcc attgattccc ccgtatttct
     1261 tgatagcgac ggcactgtcc gttcctgaag aaaagaagat aacaagatga aaaagctatt
     1321 gaagataggg cttcccgttg tagcagttgt cgcggcgctt gcggtttact tctttcactt
     1381 cgccaacaag accgaggtcg catcccagaa gcccgaagtg cgtattcccc ttgtgaatat
     1441 cgctccggtc cgcgcccagt ccatgaatat cccgctattt acccggggca ccgtcacacc
     1501 cagcacccag atccatcttg tatccgaagt ctccggacag atcgtcgaga tttcgccgaa
     1561 tttttctgcg ggtggctttt tcaagcgcgg cgaagtgctg gtgcgtgtcg acaccctcga
     1621 atacgacgtc atgatcaagc gcgccgaggc tgccaaggcc caggcgtacc agaccttcct
     1681 gcaggccaag gccgaacgca aggcccgctc catggggcgc agcgtgcagg acaacacact
     1741 ggccagcttt gaagtgcagg tgcgccaggc ggaagcgcat tacctggcaa ccagttccga
     1801 actggaagcg atgaagaagc agcgcgagcg cacgaccgtg cgggcgccct ttgatggccg
     1861 ggtgctgaca gccggtctca acataggcca gtttctgcgg cctggagcgc aaatgggcgt
     1921 gatctatgcg gtggacgttg cggaagtgcg gctgccactg tcggatcgcc agctcgggct
     1981 ggtggacgtg ccggggcgga tgtccgacca ggaaacgctg gccatgcccg aagttctctt
     2041 caccgaggaa tttgcgggca aaacctttac ctggaaagga caggtggtcc gtaccgaagg
     2101 gggcgtggac gagcgcaacc gtcttttgta cgtggtggct catgtgccag atccctatgg
     2161 catcgatgcc ggtcaacctg gtcgtcccga actggtggcg ggttccttcg ttgaagccaa
     2221 aattgccggt cgcaaattcg agcgcgtctt cgccattccg cgcaaggcgt tgcgtaatgg
     2281 caatgaggtc tgggtagtga ctgacgacag tcgtctcagc aagcgcgaaa tcggcatcct
     2341 ccacaaggaa aaggataccg tctacatcaa caccggcatt gatgacggtg agcaggtggt
     2401 cctgaaccag ctggatattg cggtcgaggg catgcgggtg cgaacccagc tgcaggaaaa
     2461 tccggatcag gtggagtcag aagtcgtcgc gggggacaac ccgtttgccg gcggtcgaca
     2521 ggtcgcgccc gcgattacgg ca
//
LOCUS       DASUEG010000188         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_28285, whole genome shotgun sequence.
ACCESSION   DASUEG010000188 DASUEG010000000
VERSION     DASUEG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28285"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFX11_03555"
     CDS             <1..206
                     /locus_tag="VFX11_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX4937723.1"
                     /translation="ADHPIRVHTDPQSGEPAPYVCVRGNLDALISRAVFYQLVDMAEE
                     VTLDGVRQSYVSSAGQRFVLGTW"
     gene            complement(343..1983)
                     /locus_tag="VFX11_03560"
     CDS             complement(343..1983)
                     /locus_tag="VFX11_03560"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX4937724.1"
                     /translation="MERDSMEYDVVIVGAGPAGLAAACKLKQLSPDISVCVVEKGSEV
                     GAHILSGAVIEPRALNELFPNWKELGAPLNTPVVGDEFYFLTSQTQGIKLQHWMIPKP
                     THNDGNYVVSLGNVCRWMAQQAEGLGVEIFPGFAAAEVLFHDDGRVKGIATGDMGVGH
                     NGEHKASYQPGMELHAKYTVFSEGCRGHLGKQLIQHFKLDAGKDPQHYGIGVKELWDI
                     DPAKHKPGLVMHGLGWPLTESGSSGGWFLYHTENNQVVVGLITDLNYSNPHVSPFEEF
                     QRCKLHPTLRHYLEGGKRVSYGARAVEKGGVNSLPKMTFPGGMLIGCDAGTLNPLKIK
                     GTHTAMKSGMLAAEAIVEAVGAGKSGEELTNFDPKLKASWLWDEMYSVRNFVGAMHTF
                     GTFIGAGYLWVEQNIFKGKLPFNFRDAVPDHATLKPAAESKKIDYPKPDNKITFDRLG
                     SVFLSNTNHEEDQPCHLKLKDASIPISVNLPKWNEPAQRYCPAGVYEVLDNADGSKRF
                     QINAQNCVHCKTCDIKDPSQNINWVVPEGAGGPNYPNM"
     gene            complement(2207..>2461)
                     /locus_tag="VFX11_03565"
     CDS             complement(2207..>2461)
                     /locus_tag="VFX11_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937725.1"
                     /translation="RGRYYELLNDQVTPLRGNGRHQVEMMAVSTHLEAGDVVGIWLFG
                     YHNQYRFSHTGWFMDAAIEGTIALPLLNLAASTGPAPAVD"
BASE COUNT          469 a          702 c          747 g          543 t
ORIGIN      
        1 atgcggatca tccgatccgg gtgcacaccg atccgcaatc cggcgaaccg gcaccctacg
       61 tttgcgtgcg tggcaatctg gatgcgctga tcagccgcgc tgtgttctat cagctggtgg
      121 acatggccga agaggtaaca ctggacggag tgcgccagtc gtatgtgagc agcgccgggc
      181 agcggtttgt gctgggcacg tggtaaggcc gggcctggaa acgcgggcga tatgcccttt
      241 cgaacacagc tgcgcaagcg ccagacgttg ccggaattga aaagctgaaa caaacgcaga
      301 aacagaaaag gggccactga ggcccctttt tttgtcacaa ccttacatgt tgggatagtt
      361 cgggccgcca gcgccttccg gcaccaccca gttgatgttc tgggacggat ccttgatatc
      421 gcaggttttg caatgcacgc agttctgcgc gttgatctgg aagcgcttgc tgccgtcggc
      481 attgtccagc acttcataca caccggccgg gcaataacgc tgggccggct cgttccactt
      541 cggcaggttg acgctgatgg ggatgctggc gtccttcagc ttcaggtggc agggctggtc
      601 ttcttcgtgg ttggtgttgg acaggaacac cgaacccagg cgatcgaagg tgatcttgtt
      661 gtccggtttc gggtagtcga ttttcttgct ctctgccgct ggtttcagcg tggcgtgatc
      721 cggcacggca tcgcggaaat tgaacggcaa tttgcctttg aaaatgttct gctccaccca
      781 cagataacct gcgccgatga aggtgccgaa cgtgtgcatg gcgccgacaa aattgcgcac
      841 actgtacatc tcgtcccaca gccagctggc tttcagtttc gggtcgaagt tggtgagttc
      901 ctcaccggat ttgccggcgc ccacggcttc cacaatggct tctgccgcca gcatgccgga
      961 tttcatcgcc gtgtgggtgc ccttgatctt cagcgggttc agcgtgccgg cgtcgcagcc
     1021 gatcagcatg ccgcccggaa acgtcatttt cggcagcgag ttcacgccgc ctttttccac
     1081 tgcgcgggcg ccgtaggaaa cccgtttgcc gccttccagg tagtggcgca gggtggggtg
     1141 cagtttgcag cgctggaatt cctcaaacgg gctgacgtga ggattgctgt aattcaggtc
     1201 ggtgatcagg cccactacga cctggttgtt ctcggtgtgg tagaggaacc agccgccgct
     1261 gctgccgctc tcggtcagag gccagcccag gccgtgcatg accaggccgg gtttgtgttt
     1321 ggcggggtcg atgtcccaca gttctttcac gccgatgccg tagtgttgcg gatctttgcc
     1381 ggcgtccagc ttgaagtgct ggatcagctg cttgcccagg tggccacggc agccttccga
     1441 gaatacggtg tatttggcat gcagttccat gccgggctgg tagctggcct tgtgttcacc
     1501 attgtggccg acgcccatgt cgccggtggc aatgcccttg acgcggccat cgtcgtggaa
     1561 cagtacttcg gcggcggcga agccggggaa gatttccacg cccaggcctt cagcctgttg
     1621 tgccatccag cggcagacgt tgcccaggct cacgacataa ttgccgtcat tgtgggttgg
     1681 cttggggatc atccagtgtt gcagcttgat gccctgggtc tggctggtga ggaaatagaa
     1741 ttcgtcgccc accaccggtg tattcagcgg agcgcccagt tctttccagt tgggaaagag
     1801 ttcgttcagc gcacggggtt cgatgacggc accggacagg atatgggcac cgacttccga
     1861 gcccttttcc acgacacaga ccgagatatc cggcgacagt tgtttcagtt tgcaggccgc
     1921 cgcaagaccg gcaggaccgg cacccacgat gactacatca tattccatcg aatctcgttc
     1981 cataacgcgt ctcctcaggc tagcttatgg atggatgggg aaggcgaccg gcgaaatccc
     2041 agggaaacca atggtttctg cgctgcaact gcaaatacgc ccgaaaaatt caaacgggcg
     2101 tattatatga aagaagcgga acaaaaccaa tgaattgccg agcgggcacc ggcggccctg
     2161 tggtcaatcg tcgcaacctg ctggccggcg gtggattctg tggcgttcaa tccacagcag
     2221 gtgccggccc ggttgatgcg gcgaggttca gcaacgggag cgctatcgta ccttcaattg
     2281 cggcgtccat aaaccacccg gtgtggctga atcgatactg attgtgataa ccgaacagcc
     2341 agatccccac cacgtcacct gcctcaagat gggtgctgac agccatcatc tccacctgat
     2401 ggcggccgtt accccgcagt ggcgtgacct gatcgttcag caattcgtag taacgacccc
     2461 g
//
LOCUS       DASUEG010000189         9197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_766784, whole genome shotgun sequence.
ACCESSION   DASUEG010000189 DASUEG010000000
VERSION     DASUEG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766784"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFX11_03570"
     CDS             <1..598
                     /locus_tag="VFX11_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4937726.1"
                     /translation="MLAPNSSNLQPWEFYVVRSPELKAKLAEACLGQNAATTANVLIP
                     IIARTNTWKKNSLRNIEYWPEETMPKIVKTYYSKVAPIHYNQGPLGALGLAKKAAGLI
                     AGLKRAVPRGPYSIAEMKAWAVKSTALAAENMMLGFRAHGFDTCPMEGFDERRVRKLL
                     KLPDDAIVTMILGVGRRAPNGIYHRRYRFPREEFVHYL"
     gene            complement(599..1513)
                     /locus_tag="VFX11_03575"
     CDS             complement(599..1513)
                     /locus_tag="VFX11_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEX4937727.1"
                     /translation="MKVAVVGLGKVGSSLAFVLAIKNFVQELVLVGRTRESVLGDVLD
                     LRHGQLFVDAPTRIVAGTVEDTAGSDIIAICASAPTPRDMQSRLQLAPANVALLRALL
                     PDLARLSPQCKMVLVSNPVDVLVHFALQITGFKPNQIMGTGTLVDSARFRQLLAEEIR
                     IHQEDIRAYILGEHGDSQFPAMSCADAGGEPIDPTPARYALFEQASRAGFEVFKHKGH
                     TNYAIALAAAFIIQCIALDSRHTMPISLKVDGFLGVDDVCLSLPAVVGKNGVERVLHP
                     SLNAAEQQAFLRSAAIVRSTIAKVAMDA"
     gene            1738..3255
                     /locus_tag="VFX11_03580"
     CDS             1738..3255
                     /locus_tag="VFX11_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX4937728.1"
                     /translation="MTTSYGVVAGRSDGQVTRFYGIPYAQPPVGDLRWADPLAPQPWD
                     GVRDAAKMGPGCTQYGVGMPVFNADEDCLTLNIWVPNTPGPHPVMVWVHGGGQMGGAS
                     SEPQYNGRSLAVSQNVMVVSVNYRLLTSGFFALPATGSRPAISGNQAIKDLIAALQWV
                     HAEIAPFGGDPANVTVFGESAGSTNVCALLATPKTQTPQRLLNRVILQSGACDTLGIM
                     TLEQAQQEGLNLLQKLGCTDAAEPLDCARAAPIKTLRDLTKMNMLKSLTYRLDEWRFR
                     IGLVIDGDVFPDNPLTLLAQDTRPDTPVLLGSNRDEGSLFAGFLDHPDDAAGYRDFLE
                     SRYPGQGDVLATLYPFENYRNAGDAHAALRGDLIMKCPTLNMARTYSVHNTTWHYYFT
                     QDVSSLTLGTVSLLFGRNPPRLGTFHGSDIGFIFDFPLATSSYQKSDRAVKNFMRQAW
                     GNFARTGNPNGPGLAQWEPFDAARNNYLELKYPPENRDGFRGNACDYWFETGYGF"
     gene            complement(3268..5091)
                     /locus_tag="VFX11_03585"
     CDS             complement(3268..5091)
                     /locus_tag="VFX11_03585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937729.1"
                     /translation="MVLSAVRLCLLLAFAGWYTPLLAAPVAEFGTGQRVLALGPFAAT
                     LEDPARTLDLSSLTGGSHAWQPLMADSLNPGFSESAWWLRVQVRNGGAQVLNALLDTG
                     TPLVDYLDLHVVREDGSIQSVVTGDRLPFSTRPMDTRTFVLPLALAPGERAWIYLRQS
                     TWDGLHEAIRLQLWNAQDYPEQLQFETLLFGLYFGTLGAILLYNLFLYLSTRERGFLL
                     YLLYVALFFVWSFAFRGYGLQYWWPQSPALNNQALPVVSSLCYVSFGLFALEYLKVKR
                     DAPRWLYRANCTGILLNGVAMIPALLGTYALAFACAVPIGAFMLTMALLAGSLLWHRG
                     SRPARYFMLAFTLLALGVMLYYVQMLGWVSAGPLTEYGLQAGSSLEVLLLALGLADQM
                     NTLKADKLKAEQQARAAQLALNTQLSIEVRQRTEELELANRRLGELAITDELTGAFNR
                     RHFNQVFEAEFAAHRRLQTPFAFCMIDIDRFKQYNDQCGHQAGDDVLRQISQCLREQL
                     RRSKDCLFRLGGEEFAILLCMDQPPEKSLPYMEKLRQSIERLALPHPGNSAGVVTASF
                     GLLTLAGTSQLERSEEAYARADALLYQAKAAGRNCVIHAAG"
     gene            5269..5823
                     /locus_tag="VFX11_03590"
     CDS             5269..5823
                     /locus_tag="VFX11_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937730.1"
                     /translation="MNLVRSATLALTLLTTLSACAQNASTTTSTQSVTGSAATAVATP
                     VAGSAPMTASDMKILADKIKADKRLLVAANMQLTDAEAKGFWPLYDAYQKELEAINVN
                     LVKGIADYADASAKGPLSDAEAKNLVDNSLKVEASELELKRSYVPKLGKVLPAMKVAR
                     YLQIENKIRAVIRYELASKIPLVQ"
     gene            5935..6399
                     /locus_tag="VFX11_03595"
     CDS             5935..6399
                     /locus_tag="VFX11_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014505316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEX4937731.1"
                     /translation="MNNNELLRLLRKLLTANETAMAEVFALGEAAVEPDTARALLKHE
                     NEPGFAPCNDEMLARFMEGLVIRQRGRKDPATPLAVEWPLDNNRLLKKLRVAFELQDE
                     AMHAILAESGQELSKQELNALFRSPGHKNFQPCSDQVLRAFIKGLTQRVRDH"
     gene            6476..7942
                     /locus_tag="VFX11_03600"
     CDS             6476..7942
                     /locus_tag="VFX11_03600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4937732.1"
                     /translation="MITDDLISVPGYTVHRLLGKGGMAAVYLATQNSLEREVAIKVLL
                     NPGDEQFNQRFVSEARIIATLNHPRVITVYDVAQLDDGRHYIAMEYLSGGDLSRLRGQ
                     MLGPLYALELIRQIAEGLSVVHRRGIIHRDIKPANILFREDGTVVLTDFGIAKDLQQD
                     CDLTQAGVSVGSPSYSSPEQSHCEPLDQRADIYSLGVILLELLVGSNPFKGDNFTQTV
                     LNHAQMPVPELPAPLRQYQPLLNRMLAKNPAERFASTDELLTVLTDLVHGQTVPSDLS
                     ITRQHFAPVRPPEPEQTGRKIVFALAVTLLLALLGGGIFWFTYESETEKKVNDLLALA
                     EQRLTEAKLTEPEFDNARYFFNEVLVFDPQNTAALEGLERVRQMQITQLLKLGAERFE
                     QQRLTRPLRDNAYFYYQQVLLLDPESAVAREGLSRITAEYIVLAQQAFSGRDYGAGHN
                     YVNLGLEVEPENATLLELKDRYKYQSNAIQRFFNRVFN"
     gene            complement(7961..9052)
                     /locus_tag="VFX11_03605"
     CDS             complement(7961..9052)
                     /locus_tag="VFX11_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4937733.1"
                     /translation="MPTDGSSTTPTLPLFDEPVLREILGQFFVASFNAILITGSEPGY
                     PILYANPAFCRMTGYTADELRGQSPAIFQGEKSNPRILHRLHEDLQAGRSFHGAAINY
                     RKNGEPYPVEWNITPVRDPTGRITHFISVQKDLSDLRQLMSRFKYTNENFRAFLRDLP
                     LSALPAAPATDLDGRKQQLTEALLDNVRLYNPALRSDEQVALFDETEFFDLSEGFTGV
                     LGEQLEQEFISAEQYALRQPVSADELDELQELLREIQQQLDLLQIGSNKSAALTNIAA
                     CLQDVASSIFYLEDFVSLSSVLAELATHTRAHAQAALPDFLVDTYRGLAQDMETWVER
                     IFVSRSAANIHEMDASIISSAKQLLMFLP"
BASE COUNT         1838 a         2860 c         2784 g         1715 t
ORIGIN      
        1 gatgctggcg cccaattcca gcaatctgca gccgtgggag ttctacgtgg tgcgctcgcc
       61 ggaactgaaa gcgaagctgg cggaggcctg cctggggcag aatgccgcca ccaccgccaa
      121 cgtgctgatt cccatcattg cccgcaccaa tacctggaaa aagaacagcc tgcgcaatat
      181 cgaatactgg ccggaagaaa ccatgccgaa aatcgttaag acctactatt cgaaggtggc
      241 gccgattcac tacaaccagg gtccgctggg ggcactgggg ctggccaaga aggcggcggg
      301 gctgattgcc ggcctgaaac gcgcggtgcc gcggggccct tacagcattg ccgagatgaa
      361 agcctgggca gtgaaatcca ccgcgctggc ggcggaaaac atgatgctgg gttttcgcgc
      421 ccacggtttt gacacctgtc ccatggaagg atttgacgaa cgccgtgtgc gcaagctgct
      481 gaaattgccg gacgacgcca tcgtcaccat gattctgggc gtaggccggc gcgcgccgaa
      541 cggtatctat caccgccgct accgctttcc gcgcgaagaa ttcgtgcact acctgtaatc
      601 aggcatccat cgcaaccttg gctatcgtgc tgcggacaat cgcggcgcta cgcaggaacg
      661 cctgttgctc cgctgcattc aggctggggt gcagcacgcg ctcaacgcca ttttttccca
      721 ccaccgccgg caggctcagg cacacatcgt ccacgccgag aaatccatcc actttcaggc
      781 tgatgggcat ggtgtgccgg gaatcgagcg caatgcactg aatgatgaaa gccgccgcca
      841 gcgcgatggc gtaattggtg tggcccttgt gtttgaatac ttcaaatccg gcgcgggaag
      901 cctgttcgaa cagcgcatag cgcgcaggcg tggggtcgat gggttcgccg ccggcatcgg
      961 cgcagctcat ggcgggaaac tggctgtcgc cgtgttcgcc caggatatac gcgcggatgt
     1021 cttcctggtg tatccggatt tcttccgcca gcagctggcg aaaccgcgcg gaatccacca
     1081 gcgtgccggt gcccatgatc tggttgggtt tgaagccggt gatctgcagc gcgaaatgca
     1141 ccagcacatc caccggattg gacacgagca ccattttgca ctgcggcgaa agccgcgcca
     1201 gatccggcag cagtgcgcgc aacagcgcca cgttggccgg cgccagttgc agccggcttt
     1261 gcatgtcgcg gggcgtgggg gcggacgcgc agatcgcgat gatgtcggaa ccggcggtgt
     1321 cctccacggt gccggcgacg atccgggtgg gtgcgtccac gaacagctgg ccgtggcgca
     1381 ggtccagcac atcgcccagc acggattcgc gggttcttcc caccagtacc agttcctgca
     1441 caaaattctt gatggccaga acaaacgcca ggctcgatcc cactttgccc aatccgacga
     1501 cggctacttt cattggcttc cccgttgctg ctaaaaaatg gagtatacga aacctgccgc
     1561 caccagcacc ccttttgtac aatactcgcc aaccattctg tcctacactc cgtcaaataa
     1621 caaaaataac ggagttgctt tcccatgccc gcctgcatct ttttgccccg gactattttc
     1681 tgtttcctgg ttttcgcact gactggcatg atcgttggct gaggcggcac ggtggacgtg
     1741 accacctcct atggtgtggt ggcgggccgc agtgatggcc aggtgacgcg cttttacggc
     1801 atcccctacg cgcagccgcc ggtgggcgat ttgcgctggg ccgatccgct ggcgccgcaa
     1861 ccctgggacg gcgtgcgtga tgcggcaaag atgggccccg gctgcaccca gtatggcgtg
     1921 ggcatgccgg tgttcaacgc tgacgaggat tgcctgaccc tgaatatctg ggtgccgaac
     1981 acgcccgggc cgcacccggt gatggtgtgg gtgcacggcg gcggccagat gggcggtgcc
     2041 agcagcgaac cgcagtacaa cggccgttcg ctggcggtgt cgcagaatgt gatggtggtg
     2101 agtgtgaact accggctgct gacgtcggga ttctttgcgc tgccggccac cggatcgcgc
     2161 ccggccatca gcggcaatca ggccatcaag gatctgatcg ccgcgctgca gtgggtccat
     2221 gctgaaattg caccattcgg cggtgatccc gccaacgtca ccgtgttcgg tgaatccgcc
     2281 ggttccacca acgtctgcgc gttgctggcc acgccgaaaa cgcaaacgcc gcaacggctg
     2341 ctcaaccgcg tgattctgca aagcggcgcc tgcgacacgc tgggcatcat gacactggaa
     2401 caggcgcagc aggaaggcct caacctgctg cagaaactcg gctgcactga cgcggccgaa
     2461 ccgctcgact gcgcccgcgc cgcgccgatc aaaacgttgc gcgatctcac caaaatgaac
     2521 atgctgaaat ccctgaccta ccggctcgat gagtggcgct tccgtatcgg actggtgatc
     2581 gacggcgacg tgtttcccga taacccgctc accctgctgg cccaggacac ccgtcccgac
     2641 acgccggtcc tgctgggcag caaccgcgat gaaggcagcc tgtttgccgg ctttctcgat
     2701 catccggatg acgcggccgg ctaccgcgat tttctggaat cccggtatcc cggccagggc
     2761 gatgtgcttg ccacgctgta cccgttcgag aattatcgca atgccggtga tgctcacgcc
     2821 gcgttgcgcg gtgatctcat catgaagtgc ccaaccctga acatggcgcg cacctacagc
     2881 gtccacaaca ccacgtggca ttattacttc acccaggatg tgtccagcct gacgctgggc
     2941 accgtgtcgc tgctgtttgg tcgcaatccg ccgcgcctgg gcaccttcca tggttccgac
     3001 atcggtttca ttttcgattt tccgctggcc acttcttcgt accagaaatc cgaccgggcg
     3061 gtgaaaaatt tcatgcggca ggcgtggggc aatttcgccc gcaccggcaa tcccaacgga
     3121 cccggcctgg cgcagtggga accgttcgat gccgcgcgca acaattatct ggaactgaaa
     3181 tatccgccgg aaaatcgcga cggctttcgc ggcaatgcct gtgattactg gtttgaaacg
     3241 ggctacgggt tttgagccac tctgtcttta accggccgcg tgtatgacgc agttgcggcc
     3301 ggcggccttg gcctggtaga gcagggcatc ggcgcgggcg taggcctcct cagagcgttc
     3361 cagctgtgac gtgcccgcca gcgtcagcag tccgaaactg gcggtgacca cgccggcgct
     3421 gttgcccgga tgcggcagcg ccagccgttc tatggactgt cgcaactttt ccatgtaagg
     3481 cagggatttt tccggcggct gatccatgca caacaggatg gcaaattcct ctccgcccaa
     3541 ccggaacagg caatccttgc tgcggcgcag ctgttcccgc agacactggc tgatctggcg
     3601 cagtacgtcg tcgccggcct ggtggccaca ctggtcgttg tactgcttga accggtcaat
     3661 gtcgatcatg cagaacgcga acggtgtttg cagacggcgg tgagcggcaa attccgcttc
     3721 gaacacctga ttgaagtggc gccggttaaa cgcgccggtg agttcgtcgg taatcgccag
     3781 ctcgcccagg cgacggttgg ccagttccag ttcttcggta cgctgtctca cctcgatcga
     3841 cagctgggtg ttcagcgcca gctgcgccgc gcgggcctgc tgttcggctt tcagcttgtc
     3901 ggccttgaga gtattcatct ggtccgccag gccgagggcc agcagcaaca cctcaaggct
     3961 ggagccggcc tgcaggccgt attcggtcaa cggcccggcg gacacccagc ccagcatctg
     4021 gacgtagtac agcatcaccc ccagcgccag cagcgtaaat gccagcatga aatagcgggc
     4081 cggccgcgat ccgcggtgcc acagcaggct gccggccagc agcgccatcg tcagcatgaa
     4141 ggcgccgatg ggcaccgcgc aggcaaacgc cagcgcatag gtgcccaaca gcgccgggat
     4201 catggcaacg ccattgagca ggatgcctgt gcagttggcg cggtacagcc agcgtggcgc
     4261 atcccgtttc accttcaggt attccagcgc gaacagcccg aagctgacgt agcacagcga
     4321 cgacaccacc ggcagggcct gattgttcag cgccggcgac tgcggccacc agtactgcag
     4381 gccatagccg cgaaaggcga agctccagac aaagaacagt gccacgtaca gcaggtacag
     4441 caggaacccc cgttcccgcg tggacaggta caggaacagg ttgtacagca ggatggcccc
     4501 cagggtgccg aaatagaggc cgaacagcag cgtctcgaac tgcagctgct caggataatc
     4561 ctgcgcattc cagagttgca gccggatcgc ctcgtgcagc ccatcccagg tggactggcg
     4621 caggtagatc cacgcgcgct cccccggcgc cagcgccaac ggcagcacaa aggtgcgggt
     4681 gtccatgggg cgggtgctga aggggagacg gtcgccggtc acgacactct ggatgctgcc
     4741 atcctcccgc accacatgca ggtccaggta gtccaccagt ggcgtgccgg tatccagcag
     4801 ggcattgaga acctgggcgc ccccgttgcg cacctgcacc cgcagccacc aggcggattc
     4861 ggaaaacccc gggttcaggc tgtcggccat taggggttgc caggcgtggc tgccgccagt
     4921 cagtgaggac agatccagcg tcctggccgg gtcttccagc gttgccgcaa acggacccag
     4981 cgccagcacc cgttgcccgg ttccgaactc cgccaccggc gcagccagca ggggggtgta
     5041 ccacccggca aaagccagca acaggcacag gcggacagca gataaaacca tggaagcgct
     5101 caggggcggg aagtggtgga gtacatggat tcagtctaga tgaaccaatc cgggcacgga
     5161 tcaataaacg caatggtttt gtaccggacg gcttgtttgc cggcagccga aaccctaacc
     5221 tcggcccgag agtaagccct tgccgcctga acaaatggag acttgtcgat gaacctggtc
     5281 cgatccgcta cccttgccct gaccctgctc accaccctga gcgcctgcgc ccagaatgcc
     5341 tctaccacca cgtccaccca gtcggtgacc ggcagcgccg caaccgccgt cgccacgcct
     5401 gttgccggca gtgctcccat gacggcctcc gacatgaaga tcctggcgga caagatcaag
     5461 gccgacaaac gcctgctggt ggccgccaac atgcagttga ccgacgccga agccaagggc
     5521 ttctggccgt tatatgacgc ctatcagaaa gaactggaag ccatcaacgt gaatctggtg
     5581 aagggcattg ccgactatgc cgacgcgtcg gccaagggcc cgctgagcga tgccgaggcc
     5641 aaaaacctgg tggacaactc cctgaaggtc gaagccagcg aactggagct gaagcgcagc
     5701 tatgtgccca agctgggcaa ggtattgccg gccatgaaag ttgcccgtta cctgcagatc
     5761 gaaaacaaga tccgtgctgt cattcgctac gaactggcca gcaagatccc gctggtgcag
     5821 tgagacagtt gcaggcctgg tgtgtcagcg ccgggcctga atccgcgctt gtcaggtccc
     5881 ggggtgctcc cccatacttc cccgcctgat gccgccaccc ccgatagatt tcccatgaac
     5941 aacaatgaac tgctgcgtct gctgcgcaag ctgctgacgg ccaacgaaac tgccatggcg
     6001 gaggtctttg ctctgggcga ggctgccgtg gagccggata ccgcacgtgc tttgctgaag
     6061 cacgagaacg agccgggttt tgcgccctgc aacgatgaaa tgctcgcgcg ttttatggaa
     6121 ggactggtga tccgtcagcg cggacgcaag gaccccgcca cgccgctggc agtggaatgg
     6181 cccctcgaca ataaccgact gctgaaaaaa ctgcgcgttg cctttgaact gcaggacgag
     6241 gccatgcacg ccatcctggc agaatccggc caggaactgt ccaaacagga gctgaatgcc
     6301 ctgtttcgca gccctgggca caagaatttt caaccctgtt ccgatcaggt tttgcgcgcc
     6361 tttatcaaag ggctcacgca acgcgtgcgc gaccactgac ggccctccgg atcgtgctac
     6421 aatcccctcg catttttcgt agtacatact gacgcggtcc agcatttcgc cagccatgat
     6481 caccgatgac ctgatttccg taccgggcta caccgtccac cgcctgctgg gcaaaggtgg
     6541 catggcagcg gtgtatctgg cgacccagaa ttccctggaa cgggaagtgg ccatcaaagt
     6601 gctgctcaat cccggcgacg aacagttcaa tcagcggttc gtcagtgaag cccgcatcat
     6661 cgccaccctc aatcatcccc gcgtgatcac cgtgtacgac gtggcgcagc tggacgacgg
     6721 ccgccactac atcgccatgg aatacctcag cggcggcgac ctgtcccggc tgcgggggca
     6781 gatgctgggc cccctgtacg cgctggaact gattcgccag atcgccgaag gcctgagcgt
     6841 ggtgcaccgg cgcggcatca tccaccgcga catcaagccc gccaacatcc tgtttcggga
     6901 agatggcacg gtggtgctga cggatttcgg cattgccaag gatctgcagc aggattgcga
     6961 cctgacccag gccggcgtct cggtgggcag cccgtcctat tccagcccgg aacaatccca
     7021 ttgtgaaccg ctggaccagc gcgccgacat ttacagcctg ggggtgatcc tgctggaact
     7081 gctggtgggc tcgaatccgt tcaagggcga caacttcacc cagaccgtgc tcaatcacgc
     7141 ccagatgccg gtaccggaac tgccggcgcc cctgcggcaa taccagcctc tgctcaaccg
     7201 gatgctggcc aagaatcccg cggaacgttt cgcctccacc gacgaattgc tgacggtgct
     7261 gacggatctg gtgcacggcc agaccgtgcc gtcggacctg tcgatcacgc gccagcattt
     7321 tgcgccggtg cggccaccgg aaccggagca gaccggccgc aagattgttt ttgccctggc
     7381 ggtgacgctg ctgctggcac tactgggcgg cggcattttc tggttcacct atgagtccga
     7441 gaccgagaaa aaagtcaacg atctgctggc gctggcggaa cagcggctga cagaagccaa
     7501 gctcaccgag ccggagttcg acaacgcgcg ctatttcttc aacgaggtgc tggtgttcga
     7561 tccacagaac accgctgcac tggaaggact ggaacgggtg cggcagatgc agatcaccca
     7621 gctgctgaaa ctgggtgccg aacgcttcga gcagcagcgc ctgacacggc cgctgcggga
     7681 caatgcctat ttctactacc agcaggtgct gctgctggac cctgaaagcg cagtggccag
     7741 ggaaggactg tcccgcatta ccgccgagta catcgtgctg gcgcagcaag ctttcagcgg
     7801 ccgcgattac ggcgccggac acaactacgt gaatctgggc ctggaagtgg aacccgagaa
     7861 cgccaccctg ctggaactga aggatcgcta caagtaccag tccaacgcca tccagcgttt
     7921 tttcaaccgc gtctttaact gaccggcatt cgcgccgcgc tcagggcagg aacatcaaca
     7981 gctgcttcgc cgatgagatg atgctggcat ccatttcatg gatgttggcg gcgctgcggc
     8041 tgacaaaaat ccgctccacc caggtttcca tgtcctgggc caggccgcgg taggtgtcca
     8101 ccagaaaatc cggcaacgcc gcttgcgcgt gagcgcgggt gtgagtggcc agttccgcca
     8161 gcaccgacga caggctgacg aaatcctcca gataaaagat cgagctggcg acatcctgca
     8221 ggcaagccgc gatgttggtc agtgcggcgg atttgttgga accgatctgc agcagatcca
     8281 gttgctgctg gatttcccgc agcaattcct gcaattcatc cagttcgtcg gcgctgaccg
     8341 gttgtcgcag tgcgtactgt tcggcgctga tgaattcctg ctccagttgt tcgcccagca
     8401 cgccggtaaa accttcgctc agatcaaaaa attcggtttc atcgaacagc gccacctgtt
     8461 catccgaacg cagcgccgga ttgtacaggc gcacgttgtc cagcagggct tccgtcaatt
     8521 gctgctttct gccgtcgagg tcggttgcag gcgcagcagg aagagcggaa agcggaagat
     8581 cccgcaggaa ggcacggaaa ttttcattgg tgtatttgaa gcgggacatc aactggcgca
     8641 agtccgacaa atctttctgc acggaaatga aatgggtgat gcgtcccgtg ggatcgcgca
     8701 cgggcgtgat gttccattcc accggatagg gctcgccgtt cttgcggtaa ttgatggcgg
     8761 cgccgtgaaa gctgcgcccg gcctgcaggt cttcatgcag ccggtgcaga atgcgcggat
     8821 tgcttttttc gccctggaat attgcgggcg actggccgcg caattcatcc gcggtgtatc
     8881 ccgtcatgcg gcagaaggca ggattggcgt acaggatcgg gtagccgggt tcgctgcctg
     8941 taatcaggat ggcgttgaag gaggcgacga aaaactgccc cagtatttcg cgcaaaaccg
     9001 gttcatcgaa tagcggcaat gtgggggtgg tgctggaacc gtctgtcggc atggtggagg
     9061 ctccggcgct ggtcagcccg gtcccatttc ccggttcttg atctgctcga acagcgccag
     9121 tgtgatgcgt tgttcaccgc gttcgcgggc agccttttcc actttggcgc gggcgagtgg
     9181 gcgaataaaa aatgggg
//
LOCUS       DASUEG010000190         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_7793, whole genome shotgun sequence.
ACCESSION   DASUEG010000190 DASUEG010000000
VERSION     DASUEG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7793"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFX11_03610"
     CDS             complement(<1..601)
                     /locus_tag="VFX11_03610"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX4937734.1"
                     /translation="MRANHESRFVRETDADRSAVLKAIVTPRLMISWLAPNVDAGAES
                     VKAAVAQPLLVEVNICCDGHEQLSAQLMFQPAGSEEGICVPLTQQDEAKWSAVIYPTQ
                     TGVHRLMVEAWVDDWSTYCKALQKKRAAHLDIRLDVQEGLLLLEQLAGAAAPPRRKQA
                     LELLQMGSELAHATVADACERLLGDQIARQVQSLKHRSFL"
     gene            complement(585..2042)
                     /gene="glgC"
                     /locus_tag="VFX11_03615"
     CDS             complement(585..2042)
                     /gene="glgC"
                     /locus_tag="VFX11_03615"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017220124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX4937735.1"
                     /translation="MFKKQGVHPSLNISSMCGDTLALIMAGGKGTRLHQLTLRRAKPA
                     VHFGGNYRVIDFTLSNCVNSGIHRIGVLTQYKSHSLIKHLQSGWSSLVARPGAFIEFL
                     PADQFPENDRWYAGTADAVYQNIECIRAHRPRNVLILAGDHIYKMDYGPMLAHHVAMR
                     AQVTIGCIEVPLAEAHDFGVIDMQADTRITRFTEKSPSPKSMPGKPDHALASMGIYVF
                     DTQFLLECLDRDAAKNVSSRDFGCDVIPEAIRAGRVFAFPFRDSSDESRPGYWRDVGT
                     IDTYWLANMELRGDRPKLDLYDESWPIWTHREWVPPAKFTVDAGGNSGTAMDSLVSGG
                     CIVSGAAVIRSVLFVRSCVDAGSVVEDSLLLPGCRVGRGCHIRKAIIDAGCVIPDGMT
                     VGINPEADALRFYRSPCDVTLVTRAMVEAVSSPAKSQYQQQSPAPPPVSPLLSDESTV
                     ELHSIVSRLDHAVLEPLSERRDWVHSGRRGCARKS"
     gene            complement(2062..2436)
                     /locus_tag="VFX11_03620"
     CDS             complement(2062..2436)
                     /locus_tag="VFX11_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937736.1"
                     /translation="MEADRHSILQTNTISTHSTESAPSCLFPPLFGSLLCRFDVAGSG
                     WDGIRWPSIELIAGILGKKILQSAAVFLRLHGKYYSAASLQHPSKGRNDAHPEDLIHF
                     LRVAHPLLPCFHNDPIRFLAEG"
     gene            complement(2518..>3548)
                     /locus_tag="VFX11_03625"
     CDS             complement(2518..>3548)
                     /locus_tag="VFX11_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4937737.1"
                     /translation="SRVDRPIDLKYEINTLREELRRLGRFGCLLGSSPAMQHVYDQIG
                     RVAPTSATVLITGESGTGKELVAQTLHNLSSRHSAPFLAINCGAISPQLIESEMFGHE
                     KGSFTGATREHKGYFERAHSGTLFLDEVTEMPVELQVKLLRVLETGFFMRVGSEREIE
                     TDIRVIAATNRSPEEAVANGKLREDLLYRLQVFPLQLPPLHARGDDVVQLAQHFLAEL
                     NNGEQVKRSFTPEVLARLKGYNWPGNVRELKNVVHRAFIMANDVIDESCLPAEFGGGS
                     APKGPSFMVRVGCSIADVERRLIQSTLDEYGGDKELAAEVLGISLKTLYNRLHEYKSS
                     ENATADSS"
BASE COUNT          779 a         1084 c          947 g          738 t
ORIGIN      
        1 ccaggaaact gcggtgcttc agtgactgca cctgtctcgc aatctggtcg cccagaaggc
       61 gttcacaggc atcagcaacc gtggcatggg ccagctcgct gcccatctgg agcagctcca
      121 gcgcctgttt gcggcgaggc ggtgcagcag cacccgccag ctgctccagc aacaacaggc
      181 cctcctgaac atcaagcctg atatccagat gggcggcacg ctttttttgc aacgccttgc
      241 aataggtact ccagtcatcc acccaggcct ccaccatcag gcgatggacc ccggtctggg
      301 tgggatagat gacagcactc cattttgcct catcctgctg cgtcagaggc acgcagatac
      361 cctcttccga cccagcaggc tggaacatca gctgggcact caactgctca tgcccatcgc
      421 agcagatatt cacttccacc agcaacggct gtgcaacggc cgccttcacc gactcagcac
      481 cggcatcgac attgggcgca agccagctga tcatcaggcg cggtgtaaca atggccttga
      541 gtacggcgct acgatccgca tccgtttccc ggacaaagcg actttcatga tttgcgcgca
      601 caccccctcc tgccactgtg cacccagtcg cgccgctctg acagcggctc cagcaccgca
      661 tgatccaggc gtgacacgat cgagtgcaat tccactgtgg actcatcgga caagagtgga
      721 gacaccggcg gcggcgccgg gctctgctgc tgatattgcg atttcgccgg cgatgaaacc
      781 gcttccacca tcgcccgcgt taccagcgtg acgtcgcagg gcgaacggta aaagcgcagc
      841 gcatcggctt ccggattgat gccaaccgtc atcccatccg gtattacgca accagcgtca
      901 atgatggcct tgcggatatg gcatccacgc cccactctgc aaccgggtaa cagcaacgag
      961 tcctcgacga cgctgccagc atcgacacag gatctgacga acaggaccga ccggatcacc
     1021 gcggcaccgg acacgatgca gcctccgctg accagcgaat ccattgccgt ccctgaattc
     1081 ccgccagcat caacggtaaa tttggcaggc ggcacccatt ccctgtgggt ccagatgggc
     1141 cagctctcgt cgtacagatc gagcttcggc cggtcaccgc gcaattccat gttggccagc
     1201 cagtaagtgt cgattgttcc aacatcccgc cagtagcccg ggcgcgattc atcgctggaa
     1261 tcccggaacg gaaacgcgaa gacccggccg gcgcggatcg cttccggtat cacatcacac
     1321 ccgaagtcac gcgatgacac gtttttcgcc gcatccctgt ccaggcattc cagcaggaac
     1381 tgcgtgtcaa acacatagat tcccatgctg gccagtgcat gatcgggttt tcccggcatg
     1441 cttttgggcg aaggggattt ctccgtgaaa cgggttatac gggtatcggc ctgcatatcg
     1501 ataacaccga aatcgtgcgc ttctgccaga ggcacttcga tacacccgat ggtcacctga
     1561 gcgcgcatgg caacgtgatg ggccaacatg ggaccgtaat ccatcttgta gatatgatct
     1621 ccagccagta tcaggacatt tctgggccgg tgggcacgga tgcattcgat attctggtaa
     1681 accgcgtccg ccgttcccgc ataccagcga tcgttttccg gaaactggtc tgccggcaga
     1741 aattcaatga acgcacccgg tcttgccacc agtgaactcc agccggactg aaggtgcttg
     1801 atcagggaat ggctcttgta ttgcgtcagg accccgatcc ggtggatacc gctgtttacg
     1861 caattggaca gcgtaaaatc aatcacgcgg tagttgccgc cgaaatgcac ggcgggcttg
     1921 gcgcggcgca aggtcaactg atgcagacgc gtgcctttgc caccggccat gatgagtgcc
     1981 agtgtgtcgc cgcacatgga gctgatattt aacgagggat gaactccctg ttttttgaac
     2041 atgccatgaa ctccactcga ttcaaccctc cgccagaaaa cggataggat cgttatggaa
     2101 acaagggagc aatggatgtg ccactcgtaa aaaatgaatg aggtcttcag gatgcgcgtc
     2161 attgcggccc ttggaagggt gctgaaggct cgcggcgctg taatatttgc catgaagccg
     2221 caaaaaaacg gctgcagatt gtaaaatttt cttccccagt attcctgcga tcaattcgat
     2281 cgacggccac cgaatcccat cccatccgga accggcgaca tcaaaacggc agagtagcga
     2341 gccgaacaaa ggcgggaaca ggcatgacgg cgctgattct gtggagtggg tactgatcgt
     2401 gttggtctga aggatgctgt ggcgatccgc ttccacagcg caacggttaa tccgttggtt
     2461 cccggtggat gtccaggcgc tgaaagccgc cactgaaaag ggggttttta ctgagagtca
     2521 tgacgaatcc gctgttgcgt tctcactcga cttgtattcg tgcagccggt tgtaaagcgt
     2581 tttgaggctg atgccaagaa cttcggccgc gagctccttg tcaccgccgt attcgtccag
     2641 cgttgactgg ataagccgtc gctcgacatc cgctatcgaa cagccaaccc tcaccatgaa
     2701 gctcgggcct ttgggtgcac ttcctcctcc gaactccgcc ggcaggcagc tctcgtcaat
     2761 gacgtcattc gccatgataa acgctcgatg cacgacattt ttcagttccc gcacattgcc
     2821 gggccagtta taccctttca ggcgggccag gacttccggc gtaaagctgc gcttgacctg
     2881 ctctccgtta ttcagctctg ccagaaaatg ttgagccagc tgcacgacgt catcgccacg
     2941 ggcatgcaac ggcggcagtt gcaacggaaa aacctgcaga cggtacaaca gatcctctct
     3001 caacttgcca ttggcaaccg cttcttcggg tgaccgattg gtagcggcga tgacacggat
     3061 gtcggtttca atctcccgct cggagcccac ccgcatgaaa aagccggttt caaggactct
     3121 gagcagtttt acctgtaact caaccggcat ttcggtaact tcgtcaagaa acagagtgcc
     3181 actgtgcgca cgctcgaaat atcccttgtg ctcgcgtgtg gcaccggtga agctcccttt
     3241 ttcatgtccg aacatctcgc tttcaatcaa ctggggcgaa atcgcgccac aattgatggc
     3301 aagaaaaggc gcactgtggc ggctgctcag attatgcagg gtttgcgcga ccagctcctt
     3361 ccccgttcca ctctcaccgg taatcagaac cgtagccgac gttggtgcca cccggccaat
     3421 ctggtcatac acatgctgca ttgccggcga actgcccagc agacagccaa agcgtcccag
     3481 acgacgcagc tcttcgcgca acgtattgat ttcatatttc agatcgatgg gccggtcgac
     3541 cctggaca
//
LOCUS       DASUEG010000191         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1640644, whole genome shotgun sequence.
ACCESSION   DASUEG010000191 DASUEG010000000
VERSION     DASUEG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640644"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1196
                     /locus_tag="VFX11_03630"
     CDS             141..1196
                     /locus_tag="VFX11_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4937738.1"
                     /translation="MRTSMRILHVAYQQWRRYGNDRRTSWVEKLDYGLIKNNHYVINY
                     SDRDVAAFEAPFGLRDLGRGRANKHLLEMVDASEPDMIILGHCDMITPETVREMRRLQ
                     PSALIAHCNCDPIFVPNNVKNIKSRASMVDAVFISTGKRALGEFEGLGARIYHMPNPV
                     DPAMEPLDNSVRSDLGIDLLFCSNATKHSTRGVTVDFIRNAVANEMNFKIYGSFGQPP
                     IWGRDYDRVLADTRMSLNLNRQEGDYWYSSDRMAQLAGNGILQFTHSSQGFQDLLPPE
                     SMVYFDSNEDLLKKVREFHHDDARRMAWAARARDFFQREMNATLYARYIVEATAQQPF
                     SHDYVWARDIHPDGRLK"
     gene            1193..1900
                     /locus_tag="VFX11_03635"
     CDS             1193..1900
                     /locus_tag="VFX11_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
                     /protein_id="HEX4937739.1"
                     /translation="MTVQLAGVAPFARGGNRLCFVHPAHPDRCIKVRRPEFTLEDLRR
                     SKGFPKNLRPLSWFDDNLEEFGVMQDIDRQTGEAAYELVSRCHGFEDTDMGRGLTSEL
                     IRDGRGTISNTLKQYLWDHGYTPALQAVLLRFAERWIALALPSRDLLLHNILVQCDKD
                     DSDIRRLVVIDGLGSAGIIPYAWLPMFLRRYMAQRKIAKLGEKIRELLAARETGTFPG
                     RMGKLFHHGQPEREKSE"
     gene            1897..2937
                     /locus_tag="VFX11_03640"
     CDS             1897..2937
                     /locus_tag="VFX11_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4937740.1"
                     /translation="MSTNPIRVAQVLAGAEHGGAENFFTRLTIALNQHPDMVQKAYIR
                     GHAHRLATLREAGVESDGFRFGGPLDILDTWRFRRALKQWKPEIVMTWMGRASQATPR
                     GDYALVNRLGHYYNLKYYRHADYWVGITKGICQHLIDGGMPAERVFNIPNFADERPVQ
                     PLPRDSFDTPADRPLLVAAGRLHVNKGFDTLLHALQKVPDATLWLAGAGPEEAALKSL
                     CQQLGLNDRVRFLGWRNDVTTLMKTADVFVCPSRHEGLGSIVMESWAHECPIVATRSQ
                     GPGEVIENEATGLLTDIDDVDQLAAALNRTLGDADLRDRLRRQAHAHYQAHYSKAVIV
                     EAYHKLYTTIRP"
     gene            2953..>3406
                     /locus_tag="VFX11_03645"
     CDS             2953..>3406
                     /locus_tag="VFX11_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEX4937741.1"
                     /translation="MTLPCWIISLNPASPTASALSSALTSQGVEHCFVPAVDGRQSRP
                     ALQGREQIDVRKSLIRHQKVLTNSELGVYLAHYRAIQKAFDEGLERVCIMEDDASLEP
                     DFAPVLQQLGELPDHVEMVRLMALRIRKRKVISTLPDGKHQLVRPERGW"
BASE COUNT          698 a         1140 c          973 g          595 t
ORIGIN      
        1 ggtcgtgaag acttgttgct ggcgcgcgtg gaggaatggc tggcacatcc gacacagccg
       61 gcggaaagcc aggacttcca gctcagcacc atgtgtgcac aaaccctggc catttatcag
      121 gccctggcca acgagaaaag gtgagaacga gcatgcgcat cctgcacgtg gcctatcagc
      181 aatggcgccg ctacggcaat gaccgccgca ccagctgggt cgaaaagctc gactacggcc
      241 tgatcaagaa caaccattac gtcatcaatt acagcgatcg tgacgtggcc gccttcgagg
      301 ccccgttcgg cctgcgcgac ctgggccgcg gccgtgcgaa caaacacctg ctggaaatgg
      361 tcgatgccag cgaaccggac atgatcatcc tgggtcactg cgacatgatc acgcccgaaa
      421 cggtgcgaga gatgcgccgc ctgcagccgt cggcacttat cgcccactgc aactgcgacc
      481 ccatcttcgt gcccaacaac gtgaagaaca tcaagtcgcg cgcgagcatg gtggatgccg
      541 tcttcatctc gaccggcaag cgcgccctcg gcgaattcga agggttgggc gctcgtatct
      601 atcacatgcc caatccagtc gatccggcca tggaaccgct cgacaactcg gtgcgcagcg
      661 acctcggcat cgacctgctg ttctgcagca atgccaccaa gcactccacc cgcggcgtca
      721 cggtggactt catccgtaac gccgtggcga acgagatgaa cttcaagatc tacggcagtt
      781 ttggccagcc ccccatttgg ggacgcgact acgaccgcgt gctcgccgac acccgcatga
      841 gcctcaacct gaaccgccag gaaggcgatt actggtactc ctcggaccgc atggcccagc
      901 tcgccggcaa cggcatcctg caattcacac acagcagcca gggcttccag gacctgctac
      961 cgccggaaag catggtctat ttcgacagca acgaagacct gctaaagaaa gtccgcgagt
     1021 tccaccatga cgacgcccgc cgcatggcct gggctgcgcg tgcccgcgac ttcttccagc
     1081 gcgaaatgaa cgccacgctg tacgcccgct acatcgtcga agccacggca cagcagccgt
     1141 tcagccatga ctatgtctgg gcgcgcgaca ttcatccgga cggccgtctg aaatgaccgt
     1201 gcagttggcc ggagtagcgc cctttgcccg tggcggcaac cgactgtgct ttgtacatcc
     1261 ggctcatccg gatcgctgca tcaaggtgcg tcgcccggag ttcaccctgg aagacctgcg
     1321 ccgctccaaa ggctttccga aaaacctgcg cccgctctcc tggttcgatg acaacctgga
     1381 agaattcggc gtcatgcagg acatcgaccg acagacaggc gaagccgcat acgaactggt
     1441 cagccgctgc catggtttcg aagacaccga catggggcgc ggcctcactt ccgagctcat
     1501 ccgcgacggc cggggcacca tctccaatac gctcaagcaa tacctctggg accacggcta
     1561 cacgccggca ttgcaggcgg tgttgttgcg ttttgccgaa cgctggatcg ccctggcact
     1621 gccctcgcgc gacttgctgc tgcacaacat ccttgtacag tgcgacaagg acgacagcga
     1681 catcagacga ctggtggtga tagacggact ggggtctgcc ggcattattc cgtatgcctg
     1741 gttgccgatg ttcctgcgcc gctacatggc gcaacgcaag atcgccaaat taggcgaaaa
     1801 aatccgtgaa ctgctggccg cccgcgagac cggcacattc cccggccgca tgggcaaact
     1861 gtttcaccat ggccaacctg aacgcgagaa atccgaatga gtaccaaccc catccgcgtc
     1921 gcccaggtgc tggcgggcgc cgagcacggc ggcgccgaaa actttttcac acgcctgacc
     1981 atcgccctca accagcaccc ggacatggtg cagaaggcct atatccgtgg ccatgcacat
     2041 cgcttggcga ccttgcgcga ggccggggtg gaatcggacg gtttccgttt tggcgggccg
     2101 ctcgacatcc tcgacacctg gcgtttccgt cgcgcgctga aacagtggaa gcccgagatc
     2161 gtgatgactt ggatggggcg cgccagccag gcgacacctc gcggtgatta cgccctggtt
     2221 aaccggctgg gccactacta caacctcaag tactaccgcc atgccgacta ctgggtgggg
     2281 atcaccaagg gcatctgcca gcacctgatc gatggcggaa tgccggccga gcgcgtgttc
     2341 aacattccga actttgctga cgaacgcccg gtgcagcccc tgccgcgcga cagtttcgac
     2401 acccctgccg accgcccgct gctggtggcc gccggccgcc tgcatgtgaa caaaggcttc
     2461 gacaccctgc tgcatgcctt gcagaaagta ccggatgcca ccttgtggct ggctggtgcc
     2521 ggcccggagg aagctgcgct caagagcctg tgccagcaac tgggcctgaa tgaccgcgtg
     2581 cgcttcctgg gctggcgcaa tgacgtcacc accctgatga aaaccgccga tgtgttcgtc
     2641 tgcccttctc gtcatgaagg gctgggctcc atcgtgatgg aatcgtgggc ccatgaatgc
     2701 cccatcgtcg ctacccgctc gcaagggcct ggcgaagtca tcgagaacga ggccaccggc
     2761 ctgctcaccg acattgacga tgtcgaccag ctggctgcag cactcaaccg cacgctgggc
     2821 gatgccgacc tgcgcgaccg gttgcgccgg caggcgcacg cgcactatca agcgcattac
     2881 agcaaggccg tgatcgtcga ggcctaccac aagctctaca ccaccattcg cccctgactc
     2941 ggaccggcca ccatgacgct gccctgctgg atcatcagcc tgaaccccgc ctcccctacc
     3001 gccagcgcgc tcagctcggc cctcacctcc caaggcgtgg agcattgctt tgtccccgcc
     3061 gtggatggcc gccagagccg cccggcattg caagggcgcg aacagattga tgtgcggaaa
     3121 tcactgatcc gtcaccagaa agtactgacc aactccgaac tcggtgtcta cctcgcgcac
     3181 taccgcgcca tccagaaagc ctttgatgaa ggccttgagc gcgtctgcat catggaggac
     3241 gacgcgtcac ttgagcctga ttttgccccg gtactgcaac agctgggcga gctgcccgac
     3301 catgtcgaaa tggtacggct gatggcactg cgcattcgca aacgcaaggt gatctccact
     3361 ttgcctgacg gcaagcacca actggtgcgg ccagagcgtg gctggg
//
LOCUS       DASUEG010000192         3911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_315172, whole genome shotgun sequence.
ACCESSION   DASUEG010000192 DASUEG010000000
VERSION     DASUEG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315172"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFX11_03650"
     CDS             complement(<1..372)
                     /locus_tag="VFX11_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX4937742.1"
                     /translation="MSKRDYYEVLGVPKGADAAEIKKAFRKMAMKYHPDRNPDDKSAE
                     AKFKEVNEAYEVLSDDQKKAAYDRFGHAGVDPSAGGGGFHGGGAGGPSFSDIFGDVFG
                     DIFGGGGGRPGGGRNNRGSDLR"
     gene            complement(676..2604)
                     /gene="dnaK"
                     /locus_tag="VFX11_03655"
     CDS             complement(676..2604)
                     /gene="dnaK"
                     /locus_tag="VFX11_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX4937743.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGDKPKVIENSEGDRTTPSIIAYNE
                     DGEVLVGQSAKRQAVTNPHNTLYAVKRLIGRKFTDNEVQKDISRVPYKIAKADNGDAW
                     VEVRGKKMAPPQISAEVLKKMKKTAEDYLGETVTEAVITVPAYFNDSQRQASKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKKRGDSIIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTHLGGEDFDLRLIEYLAGEFKKDTGIDLHKDPLALQRLKEASEKAKIELSSS
                     QQTEVNLPYITADASGPKHMNIKLTRAKLESLVEDMIVNSLKPCETALKDAGKSKSEI
                     DEIILVGGQTRMPLVQSKVKEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGDVKDVLLL
                     DVTPLTLGIETMGGVMTPLIEKNTTIPTKKSQIFSTADDNQTAVTVHVLQGERKQASQ
                     NKSLGRFDLADIPAAPRGMPQIEVAFDIDANGILNVSAKDKATGKQQSIVIKASSGLS
                     EEEIQKMVRDAESHAEEDRKFEELVTARNQADGLIHATKKTLADAADKVQPEEKSRIE
                     AAILALEAALKGDDKADIEAKTKALTEASSSLAQRLYAEQQAQPGADGAQATDAGAKA
                     KSGDDVVDAEFEEVDEKK"
     gene            complement(2769..3359)
                     /gene="grpE"
                     /locus_tag="VFX11_03660"
     CDS             complement(2769..3359)
                     /gene="grpE"
                     /locus_tag="VFX11_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX4937744.1"
                     /translation="MANEDAARAHTEAEQAPGADQSAAQQAVHEAEMLDAPSIPALEQ
                     EIAQLMARVNEQQESVLRAQAEMQNVRRRAERDVENAHKFALDKFSADLLPVVDSLER
                     ALDAAGQIDNEAVKGMKDGIELTLKMFTDTLAKHGVQQVNPLGQPFNPEFHQAMSMVP
                     NPDLAPNSVMAVLQKGYTLSGRLVRPAMVVVSKAAS"
     gene            complement(3450..>3911)
                     /locus_tag="VFX11_03665"
     CDS             complement(3450..>3911)
                     /locus_tag="VFX11_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX4937745.1"
                     /translation="LVAIRDRIVSAMKDDKTRMDSLMQTVIEVASASFGQQEQPECVV
                     SGQSHLLGMADQGGVDRLKDLFEAFQHKRDLLHLMDSCVSAQGVQIFIGHESGYKMLD
                     ECSVISSPYGNGDEVLGVLAVIGPTRIPYQKVIPMVDLTAKILSAALKPFK"
BASE COUNT          696 a         1197 c         1109 g          909 t
ORIGIN      
        1 gcgcagatcg ctgccacggt tattgcgtcc accaccggga cggccaccgc caccaccaaa
       61 aatatcgcca aaaacatcac cgaaaatatc gctgaagctg ggaccgccgg cgccaccgcc
      121 gtggaaccca ccaccgcccg cgctgggatc gacgccggca tgaccaaagc ggtcgtaggc
      181 tgcttttttc tggtcgtctg aaagcacttc gtaggcctcg ttcacttcct tgaacttggc
      241 ttcagcggac ttgtcgtcgg gattgcggtc ggggtggtac ttcatcgcca tcttgcggaa
      301 ggctttcttg atttccgctg cgtcggcgcc cttgggcacc ccgagtactt cgtaataatc
      361 tcttttcgac atgcgtgcag accgttgcgt tgtgctgacg tttggaaaga ttgccgggga
      421 tgtcgcgtgg cacgacggag gcgggtactg acttgcagga acgtcagcgg ccagggatgg
      481 ccgctgtcga gcctacaggg atgtacttgt ggcgtgtcct gcaaggcagt acccgcctcc
      541 gcttcgatcc cgctacggag atcccctaaa ttcttgctgc cctttaaaca gctgcgcgga
      601 gcatgaactc cgcggcggta ttgcctcctg cccggacctt gcagcccggg caggctaatc
      661 agaccagagg ccggattatt tcttctcgtc cacttcctcg aactcggcat ccaccacatc
      721 gtcgccggac ttggccttcg caccggcatc cgtagcctgc gcgccatcag ctccaggctg
      781 ggcctgttgc tcagcgtaca agcgctgggc cagactggaa gaagcttcag tcagggcctt
      841 ggtcttggct tcgatgtcag ccttgtcatc gcctttcagc gcggcttcca gtgccagaat
      901 cgcagcctcg atgcgggact tctcttccgg ctgcaccttg tcggcggcgt cggccaaagt
      961 cttcttggtg gcgtgaatca gaccatctgc ctggttgcgc gcggtaacca gctcttcaaa
     1021 cttgcgatcc tcctccgcat gggattcagc atcgcgcacc atcttctgga tttcttcctc
     1081 gctcagaccg gaagacgcct tgatcacaat ggactgctgc ttgccggtgg ccttgtcctt
     1141 cgcgctcacg ttcaggatac cgttggcgtc gatgtcgaac gcaacttcga tctgcggcat
     1201 gccgcgcggt gcagcaggaa tgtcagccag gtcaaaacgg cccagcgact tgttctggct
     1261 tgcctgcttg cgctcgccct gcagcacatg cacggtcact gcagtctggt tgtcatcagc
     1321 ggtcgagaaa atctgcgatt tcttggtcgg aatcgtggtg ttcttctcga tcagcggcgt
     1381 catcacgcca cccatggttt cgatacccag ggtgagcggg gtcacgtcca gcagcagcac
     1441 gtctttcacg tcaccggaca gcacggccgc ctgaattgca gcgccgacgg ccacggcttc
     1501 atccgggttc acatccttgc gcggttcctt gccgaaaaac tcttttacct tggactgcac
     1561 cagcggcatg cgggtctgac cgcccaccag gatgatctcg tcaatttcag acttgctctt
     1621 gccagcatct ttcagcgcgg tttcgcacgg cttcagcgag ttgacgatca tgtcttccac
     1681 cagcgactcc agcttggcgc gggtcagctt gatgttcatg tgcttgggac cggaagcatc
     1741 tgcggtgatg tacggcaggt tcacttcggt ctgctggctg gaggacagct cgattttcgc
     1801 tttttcagac gcttccttca gacgctgcag ggccaatggg tccttgtgca ggtcgatacc
     1861 agtgtctttc ttgaattcgc cggccagata ctcgatcagg cgcagatcaa agtcttcacc
     1921 acccaggtga gtgtcaccgt tggtagccag tacttcgaac tggtgttcgc catccacttc
     1981 ggcgatttcg atgatggaaa tatcgaaggt accaccaccc aggtcgtata cggcgatgat
     2041 ggagtcaccg cgtttcttgt ccatgccgta ggccagggcc gcggcagtgg gctcgttgat
     2101 gatgcgcttg acatccagac cggcaatgcg gccggcgtcc ttggaggcct gacgctggct
     2161 gtcgttgaag taggccggca ctgtgatgac cgcctcggtg acagtttcac ccaggtagtc
     2221 ttcagcggtc ttcttcattt tcttcagcac ttcggctgaa atctgcggcg gcgccatttt
     2281 cttgccacgc acttccaccc aggcgtcacc gttgtcggcc ttggcgatct tgtacggcac
     2341 gcgggaaatg tctttctgca cttcgttatc ggtgaatttg cggccgatca ggcgcttcac
     2401 cgcatacagg gtgttgtgcg ggttggtaac cgcctgacgc ttggcggact ggcccaccag
     2461 tacttcgcca tcctcgttgt acgcgatgat ggacggcgtg gtgcggtcgc cttcgctgtt
     2521 ctcaatgacc ttgggtttat caccctccat gattgcaacg caggagttgg ttgtgcccag
     2581 gtcaataccg ataatcttgc ccatgttgat ctccaccttg ttcagcctgc aggaggctta
     2641 ctcactcaat ttgaaactgt tgaacgtctg gttcgtgctg cgggatcctg aaatcccgtt
     2701 ccgcttgaaa cccttaatgg gggccggggc ttcattttca agacccgtgt tttcaagagg
     2761 cggacacctc aagacgctgc cttgctcacc accaccatcg ccggacgcac cagacgaccg
     2821 ctcagggtgt agcccttctg cagcaccgcc atgacggagt tgggtgccag gtcggggtta
     2881 ggcaccatac tcatggcctg atggaattcg gggttgaacg gctgacccag gggattgacc
     2941 tgctgcacgc catgcttggc cagggtgtcg gtgaacatct tcaatgtcag ctcgatacca
     3001 tccttcatgc ccttcaccgc ttcattgtcg atctggccgg cggcatccag ggcgcgctcc
     3061 aggctatcca ctaccggcaa caggtcagca gagaacttgt ccagcgcaaa cttgtgagca
     3121 ttctccacgt cccgctcggc gcggcggcgc acattctgca tttccgcctg ggcccgcagc
     3181 acgctttcct gctgctcgtt gacccgggcc atcagctgcg caatttcctg ctccagggcg
     3241 gggatggaag gggcatccag catttccgct tcgtggactg cctgctgggc tgctgactgg
     3301 tctgcgccgg gcgcctgttc ggcttcggtg tgggcgcggg ccgcgtcttc gttcgccatg
     3361 ataaaactcc agaattaaca aagggttgga tctaaaaaca aaaaaggata cgcccagaga
     3421 ctgggcaaac tgtgggggct atatggggcc tatttgaagg gcttcaaggc ggcactcaga
     3481 atttttgccg tcaaatcaac catcgggatc actttctggt agggaatgcg ggtcggcccg
     3541 atcaccgcca gcactcccag cacttcatcg ccattgccgt agggggacga aatcacactg
     3601 cactcgtcca gcatcttgta gccagactca tggccgatga agatctgcac cccctgggcg
     3661 ctgacacaac tgtccatcag gtgcagcagg tcgcgcttgt gctggaaggc ctcgaacaga
     3721 tccttgagcc ggtccacccc gccctggtcc gccatgccca gcaggtggga ctggcccgac
     3781 accacgcatt ccggctgctc ctgctggcca aagctggcgg atgccacctc gatcacggtc
     3841 tgcatcaggg aatccatccg ggtcttgtcg tctttcatgg cggacacgat gcggtcgcgg
     3901 atggccacca g
//
LOCUS       DASUEG010000193         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1507910, whole genome shotgun sequence.
ACCESSION   DASUEG010000193 DASUEG010000000
VERSION     DASUEG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507910"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2622)
                     /gene="treY"
                     /locus_tag="VFX11_03670"
     CDS             complement(<1..>2622)
                     /gene="treY"
                     /locus_tag="VFX11_03670"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX4937746.1"
                     /translation="TYRLQLNSQFTFADASAQVAYLSRLGISHVYLSPVLTARSGSTH
                     GYDVIDYGTVNPELGGEHGLRTLVAALRAHEMGVIVDIVPNHMAVGGNDNALWSDLLE
                     WGRASRSAHFFDIDWDVPDPALTNRILAPFLGETYGEALTGGKLRFGFDNVQGRFHID
                     YFEHRFPLAAMHYASLLRLGGEALRALQSRFRAVFASGHGGHRDRFMASCADLAQQYA
                     DDHAIRAAVDQLLRWFDPQTATGQRLLHQLLERQHYRLSNWRTAADEINWRRFFDIIQ
                     LAGVRIQEPAAFELVHSTIFRLYAEGLIDGIRIDHIDGLADPRTYCRRIHKRLRQLSR
                     LRPPEAPGGPAYVVVEKILAPEERLPREWQVDGTTGYSFMNDVSAMLHDPKGEPPLTE
                     LWTHLSGRFSDFEAESKQARRWILKELLAAEFNACAHALHQVARSDPMTRDWSQMAVR
                     RVLEELLVQFPIYRTYADARGRSEANTEIMLTAIAAARSNCRLVDRPLLELIDRWLGA
                     EPLPAGLPRQRRRMHLRAITRFQQLCSPLAAKSVEDTAFYRHGRLLSRNEVGANPAQF
                     ANSPQDFHNECARRATCFPRAMLATATHDHKRGEDLRARLAVLSEIPQHWSDRVQQWM
                     EANRKHKSAGQPHPADEYMLYQMLIAGWPLMLDTQDTQGLQDLCERLQQWWIKALREA
                     KQLSSWSTPNLHYEQSCQHFLKSLFDTGLAPHFLEEVSDFAHHIAPAGVANSLSQTLL
                     KLTTPGIPDIYQGCELWDFSFVDPDNRRPVDYAQRKRLLEGGPASQRDWRSGAIKQQL
                     IAQLLGLRRAHPELFLCGSYEPLEITGDRAEQVFAFARHWQQRTLVVATVRLPATLIL
                     DWPGIDPGIWGNTQLHLP"
BASE COUNT          525 a          743 c          819 g          535 t
ORIGIN      
        1 ggcaaatgta gctgggtgtt gccccaaatc ccaggatcaa taccgggcca gtcgagaatc
       61 agcgtggcag gcaggcgaac cgtggccacg accagggtac gttgctgcca gtgacgggcg
      121 aaggcgaaaa cctgttcggc acggtcaccg gtgatctcca gcggctcata gctgccgcag
      181 agaaaaagtt cagggtgtgc cctgcgcaag cccaacagct gtgcgatcag ctgttgcttg
      241 atggcacccg accgccagtc ccgttgtgaa gcagggccgc cctcaagcaa ccgtttgcgt
      301 tgggcatagt ccaccggtct ccggttgtcg ggatcgacga aactgaagtc ccagagttca
      361 cagccctggt aaatgtcggg aatgcccggt gtggtcagtt tcagcagcgt ttgggaaagg
      421 ctgttggcga cgcctgcagg tgcaatgtga tgggcaaagt cagaaacctc ttcgagaaaa
      481 tgcggcgcca atcccgtatc gaacaggctt ttcaggaagt gctggcaact ctgttcgtag
      541 tggagattgg gcgtactcca gctggacagt tgtttggctt cccgcaacgc cttgatccac
      601 cactgttgca gtcgctcgca caggtcctgc aggccctggg tgtcctgggt gtcgagcatc
      661 aggggccagc ctgcgatcag catctggtaa agcatgtatt cgtctgctgg atgcggctgg
      721 ccggcggatt tatgtttacg gttggcctcc atccattgct ggacgcgatc agaccagtgc
      781 tgtgggattt ccgaaagtac ggccagtcgt gcccgcaggt cttccccacg tttatgatca
      841 tgtgtcgcgg tggcgagcat cgcccttgga aaacaggtgg cacggcgcgc gcattcattg
      901 tgaaaatcct gcggcgaatt ggcgaactgg gccggattgg cgcccacctc gttgcgcgac
      961 aacagccttc catgacggta aaaggcggtg tcctccacag atttggcggc caatggcgag
     1021 cacaattgct gaaaacgtgt aattgcgcgc agatgcatgc gccgacgttg ccgcggcagg
     1081 ccggcgggca ggggttcggc ccccagccag cggtcaatca gttccagcag gggacgatcc
     1141 acgaggcggc agttcgaccg tgccgcggcg atggccgtca gcatgatttc cgtattggct
     1201 tcgctgcgtc cccgcgcatc tgcgtaggta cggtaaatgg gaaactgcac cagcagttct
     1261 tccagcacgc ggcgaaccgc catttgactc cagtcgcggg tcatgggatc gctgcgtgca
     1321 acctggtgta gcgcgtgagc gcatgcattg aattctgctg ccaggagctc ttttagtatc
     1381 cagcggcgtg cctgctttga ctcggcttca aaatcagaga agcgaccgct cagatgcgtc
     1441 cagagttcgg tcaggggtgg ctcgcctttg gggtcgtgaa gcatggcgga cacatcattc
     1501 atgaagctgt agccggtggt gccgtcaact tgccattccc gcggcagtcg ttcttccggc
     1561 gccaaaatct tttccacaac aacatacgca ggaccgcccg gtgcctccgg cggacgtagt
     1621 cgggacagct ggcgcaatcg cttgtggatc cgtctgcagt aagtgcgggg atcggccagg
     1681 ccgtcgatat gatcgatgcg gatgccatcg atcaggcctt cggcgtacag gcggaaaatg
     1741 gtggaatgca ccagttcgaa tgcagcgggt tcctggatgc gaacgcccgc caactggatg
     1801 atatcgaaaa agcgacgcca gttgatttca tctgcggccg tgcgccagtt ggatagccgg
     1861 tagtgctgtc gttccagcaa ctggtgcagc agccgctgcc ctgttgccgt ttgcggatca
     1921 aaccagcgca acaactgatc gacggccgca cggatggcat ggtcgtcggc atactgctgc
     1981 gcgaggtcgg cgcaggacgc catgaagcgg tcgcgatgcc ctccatgtcc cgaggcgaat
     2041 acggcgcgga atcggctttg cagcgctctc agagcctcgc cacccagccg cagcagcgat
     2101 gcgtagtgca tcgcggccag cggaaaccgg tgctcaaaat aatctatgtg aaagcggcct
     2161 tgcacgttgt caaagccgaa acgtaatttt ccgccggtca gcgcttcgcc gtacgtttca
     2221 ccgagaaacg gcgcaagaat gcggttggtg agcgcgggat cgggtacatc ccagtcaata
     2281 tcgaaaaaat gcgcagagcg gctggcgcgg ccccattcca gcagatcaga ccagagtgcg
     2341 ttgtcgttac cacctaccgc catgtgattg ggtacgatgt caacgatcac tcccatctca
     2401 tgggcccgca atgctgctac cagcgtccgt aatccgtgtt cgccgcccag ttccggattg
     2461 accgtgccgt agtcgatgac atcgtagccg tgggttgatc cgctgcgagc ggtcaacacc
     2521 gggctcagat agacgtgact gatcccgagc cgcgacagat aggcgacctg tgcgctggcg
     2581 tctgcaaatg tgaattgcga gtttagctgg agtcgatagg tg
//
LOCUS       DASUEG010000194         4776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_602176, whole genome shotgun sequence.
ACCESSION   DASUEG010000194 DASUEG010000000
VERSION     DASUEG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602176"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFX11_03675"
     CDS             complement(<1..1362)
                     /locus_tag="VFX11_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937747.1"
                     /translation="MSTPFIRSLIPGVMSLVLSAWMSGCSQAPVTGADSATQNIQQRA
                     ESGAAGANAATDFDPVQLKKDADALMQRAGKENSVALLQDAAVAYEQLLQLAPEDSSL
                     QYQLYDVYYRLAAGGADNVEPRLRALFVALPAPLRDAVRPPSLAVFLQLFEQSENSKQ
                     YDDDRLGGLLLAAIQEQPTEPATYLLLADGYLRTRHPALAIATVKQGLVHNPDHPASQ
                     KYLGQAYRAFVRSQSCVYSYRDEIQKAAESLRIASSQLPEDLKIRELLVDVYSLMNLA
                     PLSANEARKLYAERSDDVSAWLLAQSLSAIAQYEKSDELYHGLVARYPDVMYEPLAGN
                     AALQGKWDAASQHWAQHFYYSRQPAFYAALMRSVSDEQAYGNAAATKIFAVLTENMDV
                     SPWQARLREFRLGRLAADQLVATATDTCERTEAFFYAGYAQKTQGNARKAAEYFQSAV
                     NEKA"
     gene            complement(1359..3584)
                     /locus_tag="VFX11_03680"
     CDS             complement(1359..3584)
                     /locus_tag="VFX11_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX4937748.1"
                     /translation="MNYCLFRRAVWPVLGFLALFAGVPVNGFAQSARLAPPLTELVRQ
                     FPATGQNTEVIRKILHIDVDEQWQETTTAYIAVRIGDSEAARDYSQISVFYTSYYETL
                     ELDFANVLAENGTTIPLASDAVQVQTPKEDSFYQDTRQLVFSLPSIRPGAVLEFQYTR
                     KPLRAIIPGEWFSRLTPYWWEDAANGQGTRVDAVRSALMQVTAPSGKPLYLSAKSKGA
                     YPFERKHGQGRQTLTWVAQDVPKFLLESNMPFDQGVERFVEVSTIRDWRTINQWAQSL
                     IEPQINADAALQATARQLAATAATPDEKVRAVYAYVQNNIRYIFAHVGHGGYQPHAAA
                     DVVKNAYGDCKDQTVLVVTLLRAMGVDAYPALVSTKSNGYFNPDIGMVGFDHMITYIA
                     DSGSSTRWLDTTGDHVLFPGIGMQTEGQRALVINGKDAAFTLLPVRSPAQQSAHMDLD
                     FRHLPGNDVEVRFEMTLSGMFEQHFRSMWVYDQNHEKTFESFAEQIFNKAEVLEVGAR
                     NADNLWQPFSLHGKLLFKDSWEGVPKTSNLAVSVNQLVRLFAGISSLEKPAQRLTPYE
                     NYIGYRLSMRATMTAPDAQYTPMAMSAGPSIRNDFYELKQSGRQEQDRYVINLSIDFP
                     RHTTSLARYPEFYEKMNGLEDLPFWNIAFVQKTAPTLAGAAKATPATDAISSQISITR
                     QYIDKGEFEKALEAATKAVAIAPKNGEARYLLGMAQGYRGLYKESDQSFAEALKLGYK
                     L"
     gene            complement(3708..4589)
                     /gene="dapA"
                     /locus_tag="VFX11_03685"
     CDS             complement(3708..4589)
                     /gene="dapA"
                     /locus_tag="VFX11_03685"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4937749.1"
                     /translation="MFTGCFTALITPFRDGQLDEPALRKLVRFQLDHGIHGLVPMGTT
                     GESPTVSAQEHKCVIDIVVQETAGQVPVIAGAGSNNPQEAIEYARHAQQAGADAVLAV
                     AGYYNRPSQEGLYRHFKALHDATDIPVLVYNIPPRTIVDIQPDTLARIAELPRIVGIK
                     DACGDLGRICHERQRIRKPFSFLSGDDITALAYNANGGNGCISVTSNVLPAQCAQLQS
                     LCAAGHFAAALALHEQLVPLHAALFREPNPAGIKYAASLLDLCSEECRLPMVALAESS
                     RAAIGRTLADIRSAAPV"
BASE COUNT          935 a         1447 c         1404 g          990 t
ORIGIN      
        1 tgccttctca ttcacagcag actggaaata ctcggcggcc ttgcgcgcat tgccctgcgt
       61 tttctgcgcg taacccgcat agaaaaaagc ttcggtgcgt tcacaggtat ccgttgcggt
      121 ggcgacaagc tggtctgccg ccagccggcc caggcggaat tccctgagcc gggcctgcca
      181 cggagagaca tccatgtttt ccgtcagtac agcaaatatt ttcgtggccg ccgcattgcc
      241 gtaggcctgc tcatcgctga cgctgcgcat cagcgcggca taaaatgccg gttgccggga
      301 atagtaaaaa tgctgggccc aatgctggct ggcggcatcc cattttccct gcagggccgc
      361 gttgccggcc agcggttcat acatgacgtc cggatagcgg gctaccagcc catggtaaag
      421 ttcatccgat ttctcgtact gtgcaatggc ggataatgac tgagccagca accaggccga
      481 cacatcgtcg gaacgctccg catacagttt gcgtgcctcg ttggcactga gcggcgccag
      541 attcatcagg gaatagacat ccaccagcag ttcgcggatt ttcagatcct ccggcaactg
      601 gctgctggcg atgcgcaacg actccgccgc tttctggatt tcatcccgat acgaataaac
      661 gcagctttga ctgcggacga aggcacgata cgcctgcccg aggtatttct gcgatgccgg
      721 atgatccggg ttgtgaacaa gcccctgctt gaccgtggcg atagccagcg ccgggtgccg
      781 ggtgcgcagg tagccgtccg ccagcagcaa ataggtggcc ggttctgtgg gctgttcctg
      841 aatggccgcc agcagcaatc cgcccagccg gtcatcgtcg tactgtttgc tgttttcgga
      901 ttgttcaaac agctgcagga aaacagcaag gcttggcggc cgcacggcgt cccgcagggg
      961 tgcgggcaac gccacaaaca gcgcccgcaa tcgcggttcc acgttgtcgg ccccgccggc
     1021 ggcaaggcga taatagacat cgtacagctg gtactgcagg ctgctgtcct cgggtgccag
     1081 ttgcagcagc tgctcatagg caaccgcagc gtcctgcagc agcgcaaccg aattttcctt
     1141 gccggcgcgc tgcatcagcg catcggcatc tttcttcagt tgcacgggat caaaatcagt
     1201 ggcggcattc gcgcccgccg cgcccgactc tgccctttgc tggatgttct gcgttgcgga
     1261 atcggcgcct gtcacgggag cctggctgca tcctgacatc cacgccgaca gcacaaggga
     1321 catcacgccc gggatcaggc tacggataaa gggcgtgctc ataatttata tccgagcttc
     1381 agtgcttccg cgaacgactg gtccgattcc ttgtacaggc cccggtatcc ctgcgccatg
     1441 cccagcagat aacgggcctc gccatttttc ggcgcaatcg ccacagcttt ggtggccgct
     1501 tccagcgcct tttcaaattc gcccttgtcg atgtactggc gggtgatgct gatctgtgaa
     1561 ctgatggcat ccgtcgccgg tgtcgccttt gctgccccag ccagcgtggg tgccgtcttc
     1621 tgcacaaagg caatattcca gaacggcaga tcttccagcc cgttcatctt ttcataaaac
     1681 tccgggtacc gcgccagact ggtggtatgg cggggaaaat cgatcgacag gttgatcaca
     1741 tacctgtcct gctcctgacg gccgctttgc ttcagctcgt aaaaatcgtt gcggatgctg
     1801 gggcccgcag acatggccat cggggtgtac tgcgcatccg gcgctgtcat cgtggcccgc
     1861 atgctgagac gatagccgat gtaattttcg taaggcgtca gccgctgtgc cggcttttcc
     1921 agactgctga tgccggcgaa gagtcgcacc agctgattga cgctgaccgc caggttggat
     1981 gtcttcggca cgccttccca ggaatccttg aacagcagtt ttccgtgcag ggaaaagggt
     2041 tgccacaagt tgtcagcgtt acgcgcgccg acctccagca cttcggcctt gttgaagatc
     2101 tgctcggcaa aggattcaaa tgtcttttcg tgattctggt cgtaaaccca catgctgcgg
     2161 aaatgctgtt caaacatgcc cgacagcgtc atttcaaacc gcacctcgac atcgttgccg
     2221 ggaagatggc gaaaatccag atccatgtgc gcactctgct gggcaggcga tcgcaccggc
     2281 aacagggtga acgctgcatc cttgccattg atgaccagtg cgcgctgccc ttcggtctgc
     2341 atgccgatgc cgggaaacag aacgtgatca ccggtcgtgt ccagccagcg ggtgctgctg
     2401 ccgctgtcgg caatgtaggt aatcatgtga tcgaaaccca ccatgccgat atcaggattg
     2461 aaatagccgt tactcttggt ggaaacgagg gcgggatatg cgtcaacgcc catggcccgc
     2521 aacagggtta ccaccagcac cgtctggtcc ttgcaatcac cataggcatt tttcaccaca
     2581 tccgccgcgg catgaggttg atagccaccg tgccccacat gcgcaaagat gtagcggata
     2641 ttgttctgca catacgcata gaccgcccgc actttctcgt cgggggtggc ggcggtggcg
     2701 gccagttgcc gcgcggtggc ctgcagtgcc gcgtcagcgt tgatctgggg ttcgatcagc
     2761 gactgcgccc actggttgat agtgcgccag tcacgaatgg tggacacctc aacaaaccgt
     2821 tcgacaccct gatcgaacgg catattgctt tccagcagga atttcggcac atcctgcgcg
     2881 acccaggtca gcgtctgccg accctggcca tgcttgcgtt cgaacgggta cgcgcctttg
     2941 ctcttcgccg acagatacag cggcttgccc gatggagcgg tgacctgcat caaggcactg
     3001 cgcaccgcat cgacccgggt tccctgaccg ttggccgcgt cttcccacca atagggcgtc
     3061 aggcgcgaaa accattcacc gggaatgatc gcccgcaacg gcttgcgcgt gtactggaat
     3121 tccagcaccg cgcccggccg gatgctgggt aacgaaaaaa ccagctgccg cgtatcctga
     3181 tagaagctgt cttccttcgg cgtctgcacc tgcacagcgt cactggcgag gggaatggtc
     3241 gtgccgttct cggccagcac attggcaaaa tccagctcca gcgtttcgta gtaactggtg
     3301 tagaacacgc tgatctggct gtagtcgcgg gccgcttcgc tatcgccaat acggacagcg
     3361 atgtaggccg tcgtggtttc ctgccactgc tcatccacat cgatgtggag gattttgcgg
     3421 atgacttcgg tgttctgccc tgtggcagga aactggcgca ccagctcggt caggggcgga
     3481 gccagacggg ccgactgggc gaacccattg accggtacac ccgcgaagag cgccagaaaa
     3541 cccaggactg gccagacagc ccggcgaaac aaacagtaat tcatgaaatt gcctgcagga
     3601 atagcgttac gtagcacatt ggtttgatgc cacaagcctg gctattttat cgctagttga
     3661 tgccgttgtc tgccggctac cccgcccgcg cggcagccgg cccacactta tactggcgcg
     3721 gcggaacgga tatctgccag ggtccgcccg attgccgccc gtgacgattc cgccagggcc
     3781 accatgggca ggcgacattc ctcgctgcac agatccagta gggacgccgc atatttgatt
     3841 cccgccgggt tgggctcgcg gaacagcgcg gcatgcaggg gcaccagctg ttcatgcaat
     3901 gccagcgccg ccgcgaaatg gccggctgcg cataatgact gcagctgcgc gcactgcgcc
     3961 ggcagcacgt tggacgtcac ggaaatgcag ccgttaccac cgttcgcgtt ataggccagc
     4021 gccgtaatgt cgtcgccgga cagaaagctg aacggcttgc gaatgcgctg gcgctcatgg
     4081 cagatacgac ccagatcgcc gcacgcgtcc ttgatgccga cgatccgcgg cagttccgca
     4141 atgcgcgcca atgtgtccgg ctggatatcg acgatggtgc gcggcggaat gttgtagacc
     4201 agaacgggaa tatcggtggc gtcatgcagc gccttgaagt gccggtacaa tccttcctgc
     4261 gacggccggt tgtaatagcc ggccaccgcc agcacggcgt cggcaccggc ctgctgcgca
     4321 tgtcgcgcgt attcaattgc ttcctgcgga ttgttggagc cggccccggc aatcaccggc
     4381 acctgccccg ctgtttcctg caccacgatg tcaatgacgc acttgtgctc ctgcgccgac
     4441 acggtgggtg attcgccggt ggtacccatg ggcaccagcc cgtggatgcc gtggtccagc
     4501 tgaaaccgca ccagtttgcg cagcgcgggt tcatccagct ggccatcgcg gaaaggggtg
     4561 atcagcgccg taaaacaacc ggtaaacatg gttttctcct ttattgaaca tgacaggttt
     4621 ggggaacgac aggcaataaa aaacccggtg gcggattccg cgaccgggtt atggggtgaa
     4681 gacgttgagt caggaactat gtcatccaca cacgcagaac cgggccgcaa acaggtttct
     4741 tctgtttgct gcgtttgagc ggtttgcggt tgtgca
//
LOCUS       DASUEG010000195         7556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1712351, whole genome shotgun sequence.
ACCESSION   DASUEG010000195 DASUEG010000000
VERSION     DASUEG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712351"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFX11_03690"
     CDS             <1..1200
                     /locus_tag="VFX11_03690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4937750.1"
                     /translation="NSAKFDELTGLWTVGTKAGDTWTCRHFVLASGPLHVPQIPTIKG
                     LDKFKGKLFHSAQWDHNYDLNGKTVVSIGTGGSAIQYCPEIAPKVKQLYVFQRTPAWV
                     IPRDERKYPEITKQAFAKVPGLRQLYRARLYWSNESRVWPLFNPSIAKVVQKLAEGFI
                     RFQVKDPALAKKLTPDYTIGCKRILISNKYFPMFNRPNVELVTDGIREIRENSIVTAD
                     GKERPVDCIILGTGFIVDPRIYMKDFELTGLGGRDLREDWKDHAECYYGTTANGYPNL
                     WQLVGPNAGLGHNSIIFMIEAQVHYILECMKLMKERGADFMDVKADVQRRFNDELQKD
                     FKGSVWSEGGCASWYNQADGRNIALWPKSTWRFWLETLRVKASDYNFGFVEAQVAKKT
                     RKSKATA"
     gene            1540..3642
                     /locus_tag="VFX11_03695"
     CDS             1540..3642
                     /locus_tag="VFX11_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937751.1"
                     /translation="MNKQVRKRIAQLAQCVLAVSLLAGCKASTTVQMKMSVAPDTRAT
                     ASEVTIPPGSFFTNGFPTDLRIKSNGKADISDFPRRVHLLTNAYVKSVDNEVTGYSPL
                     LPIYIPFTAPILTDLMPQDEMDYVRDDAAVQLIDVDSDSPEYGRRFPLNLKMTAKADQ
                     YRPDNLLQVLPMLGVNLRPGTTYALIVTDQVPVAEDLSIVQDPQLQTLLDPESGAAAV
                     SERALAVYAPLRTLLETEGFDKDSIIGATVWTTGEMKTRMRTGAAHVAALPVASSKDV
                     KLLEEFDDYCVIQGFVDVPGFQKGAIPYPLTGGKILFNSDNTPIQQYTRSAEWIITIP
                     KRTPMPENGFPVLSFHHGAGGNAEQVFTRGAFLRRAIDIILPYSVLDRGNGPSQIAAE
                     RGWASSGFAGHMSMDHLGAEGGGYGLFGYNMFNPVAFKYTYYQMVWERIYFRRVLGNL
                     KIDASLCPDAIAGDGNSEFRIDPDMQVSMGQSLGNWTSSLQVANDPDPFEGVIFTGIA
                     GTWLLMFIDDPSRRSAFELGVVSLLPGEHLDRYHPFLALLEWVLSDVDPTLYMDEIFR
                     NPTKTAPHIMAISGIDDHGSSEVVQRTFLTALGLDLAGEELGTTPENNLLAYLQKTGT
                     QQLPYPVVNNISVPGQGQRTGAIVRYENTVRPDHNGHHVTFDIPAPKHQYGCFMQNLA
                     EGRAPVIVEGSVQNGPCL"
     gene            3828..5882
                     /locus_tag="VFX11_03700"
     CDS             3828..5882
                     /locus_tag="VFX11_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937752.1"
                     /translation="MLTVNCLRCSRFLVCVLLLLIAGCKQTIEMRMDVNAVPAARSKQ
                     FFHNGFPTDLRRQEDGSISLIDIPHGQEYFTRLYADSIQQEAIGYSPVMPVYLPFSGA
                     LQLDLLSSNETDFASADAPIQLIDIDADSPEYGRRFPLQLSMTWQADSYRPSRLLQAL
                     PSPGVNLRTHTTYALIVTDAVPVSAEDRFRQHPQLQTLLDPDSNAADVSDRALAVFAP
                     LRNFLATENIDAARIMGATVWTTGDPTAWLRRGAQHMTTLPVPAPQNVVRAEDFPDYC
                     VIRGTFAVPGFQRGTIPYPLVNYGGDIQWNEDGAPIQQYTREAKFVVTIPKHASMPDN
                     GFPVMWFNHGAGGTADQVFTRGAHIDIGVRVEGGGPAMIAAQKGWVTASMAAHMSMDH
                     LGVLNGGQGIWEYDLINPVAMRNNYAQMVWEKILFRYFIRQLRLDASLCPETDPGANR
                     VEFFLDRDREVLMSQSLGNWVGTIQTAVEPEPYIGVIYSGFAASWLRFFTDNFVKAHA
                     IETGMLALPPLEHFDQWHPFMMLLQWLAAAADPTLYAPDILRAPTKTAPHVLAISGQF
                     DRSSSEWVQQVYATALGVDLAGPELGADNKTRILEYMQQFGARQLDYPVANNLDIPGQ
                     GMRTAVVVRHFNSGRAGNNGHNVTYDIDPPKHQYGCFLQQLADGRAPVIVEGSVQNGP
                     CD"
     gene            5955..6944
                     /locus_tag="VFX11_03705"
     CDS             5955..6944
                     /locus_tag="VFX11_03705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019839433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937753.1"
                     /translation="MASPKMDTAADPAQGASPTPPDVTAPAGTEPDRPLILIIDDSPE
                     SIRLLSGLILDKGDVVFATSGEAGLTLARQRRPDLILLDVEMPDLSGYEVCRRLKDDA
                     DTRDIAVMIVTAHHSSAHEVQALEAGAIDFLTKPLAPAVVRARVQTQLTLKRQADALH
                     RQAMLDGLTGLYNRAYFHEHLELEWRRHLRQQAPLGFALLDIDYFKAFNDQYGHVAGD
                     ACLRRVAAALVASTRRPAEFVVRYGGEEFAIVMPNTPREEIGRFGEWICRQVQDLAIP
                     HSASSVAPVVTLSAGLVVLVPGTAVTWSDLIERADRALYRAKQEGRNRAVVAD"
     gene            6944..>7556
                     /locus_tag="VFX11_03710"
     CDS             6944..>7556
                     /locus_tag="VFX11_03710"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX4937754.1"
                     /translation="MLRAVSLFAVITLLALPCIMLTREAGNIAYLWLPNVVGFVVLLR
                     AGTRQWPFLLLAILAGILSAGLFYGDAFSFALGTTLANVLEILLAATLVRRFVLRPGD
                     MPGIQNALQLLGLYVLVAAPLGALLGALVVRASFGTPLPEVIGNWWLGDMIGVLLLGL
                     PGLLLRREHLRYLRQPRQVADFLLTFAILALVSYLLLRYVRFPF"
BASE COUNT         1433 a         2236 c         2330 g         1557 t
ORIGIN      
        1 aattccgcca aattcgatga actcaccggc ttatggaccg tcggcaccaa agccggcgac
       61 acctggacct gccgccattt cgtactggcg tccggtccgc tgcacgtgcc gcagattccc
      121 accatcaagg gcctggataa attcaagggc aagctgttcc actcggcgca gtgggatcac
      181 aactacgatc tgaacggcaa gactgtggtg tccattggca ccggcggcag tgccatccag
      241 tactgcccgg aaattgcgcc aaaagtgaaa cagctttatg tgttccagcg cacgccggcg
      301 tgggtgattc cgcgcgacga acgcaagtac ccggaaatta ccaagcaggc gttcgcgaaa
      361 gtgccggggc tgcgccagct gtaccgcgcg cgtctgtact ggagcaacga atcccgcgtc
      421 tggccgctgt tcaatccgtc cattgcgaaa gtcgtgcaga aactggcaga aggatttatc
      481 cgctttcagg tgaaagatcc ggcgctggcg aaaaagctca cgcccgatta caccatcggc
      541 tgcaagcgca tcctgatttc caataaatat ttccccatgt tcaaccgccc caatgtggag
      601 ctggtgactg acggcatcag ggaaatccgt gagaacagca ttgtcacggc agatggaaaa
      661 gagcggccgg tggactgcat cattctgggc accggcttta ttgtggaccc gcggatttac
      721 atgaaggatt tcgaactcac cggcctgggc ggacgcgatc tgcgcgagga ctggaaagat
      781 cacgcggaat gctactacgg caccaccgcc aacggttacc ccaacctgtg gcagctggtg
      841 ggaccgaacg cgggtctggg ccacaactcg attattttca tgatcgaggc gcaggtgcac
      901 tacattctgg aatgcatgaa gctgatgaaa gagcgcggcg ctgatttcat ggacgtgaaa
      961 gcggatgtgc agcgccggtt caacgatgaa ctgcagaaag atttcaaggg ctcggtgtgg
     1021 agcgaaggcg gttgcgccag ctggtacaac caggccgacg gccgcaacat cgcgctgtgg
     1081 ccgaaatcca cctggcgttt ctggctggaa accctgcggg tgaaagcgtc ggactataac
     1141 ttcggttttg tcgaagcgca ggtggcgaag aaaacccgca agagcaaagc cacagcctga
     1201 tagaaatcac cgccgcgccg ccgtgaccgg cagcgcgtgt gctggtagtg aggtgtgccg
     1261 actcgaacac ctgacgggcg gaccggtgac ggtgccgccc tttttcgttt cggctgccag
     1321 ttgactgatt ttccgttcat taaccgagct ataaccctcc cctgattttc ctatcagccg
     1381 cgatggccgc ccctgcggtc gcccgtcacc gggcatccgt ttacaataat caaacttttg
     1441 cggaaagaat gtcgatataa catagggcgg acttgcggtc tgtccgcgtg tgtcactgac
     1501 aacaacaaaa tcacaacaag aagcagattg gggaatgcca tgaacaagca ggttcggaag
     1561 cggattgcgc agttggcgca gtgtgtgctg gcagtcagtt tactggccgg atgcaaggca
     1621 tccaccacgg tgcagatgaa aatgagtgtg gcaccggata cccgcgccac cgccagcgaa
     1681 gtcaccattc ctcccggcag tttcttcacc aacggtttcc ccaccgatct gcgcatcaaa
     1741 tccaacggca aggccgatat ttccgatttc ccgcgccggg ttcacctgct gaccaacgct
     1801 tacgtcaaga gtgtcgacaa cgaagtcacc ggttattcgc cgctgctgcc gatctacatt
     1861 ccgttcactg cgccgatcct gacggatctc atgccgcagg acgaaatgga ttacgtgcgt
     1921 gacgacgcag ccgtgcagct gatcgacgtg gacagcgatt cgcccgaata cggccgccgc
     1981 tttccgctca atctgaaaat gaccgccaag gcggatcagt accggcccga caacctgctg
     2041 caggttctgc cgatgctggg cgtgaatctg cgcccgggca ccacttatgc gctgattgtc
     2101 accgaccagg tgccggtggc cgaagacctg agcatcgtgc aggacccgca gctgcaaacc
     2161 ctgctcgatc cggagtcggg cgcggcggcc gtttccgagc gcgcgctggc cgtgtatgcg
     2221 ccgctgcgga cgttgctgga aactgaaggt ttcgacaagg acagtatcat cggcgccacc
     2281 gtgtggacca cgggtgaaat gaaaacccgc atgcgtaccg gtgcggccca tgtggcggcg
     2341 ttgccggtgg catcgtcgaa ggatgtcaaa ctgctggaag agtttgacga ttactgcgtg
     2401 atccagggct tcgtcgatgt gccgggcttc cagaagggcg ccattcccta cccgctgacg
     2461 ggcggcaaga tcctgttcaa cagcgacaac accccgatcc agcaatacac ccgttccgcc
     2521 gaatggatca tcaccattcc caagcgcact cccatgccgg aaaacggttt cccggtattg
     2581 agtttccatc acggcgccgg tggcaatgca gagcaggtgt tcacgcgcgg cgctttcctg
     2641 cgccgcgcca tcgatatcat cctgccctat tccgtactgg atcgcggcaa cggcccgtcg
     2701 cagattgcgg cggagcgcgg ctgggccagt tccggtttcg ctggccacat gtcgatggat
     2761 cacctgggtg ccgaaggcgg tggttacggc ctgtttggct acaacatgtt taaccccgtg
     2821 gccttcaagt acacctatta ccagatggtg tgggaacgga tttatttccg ccgcgttctg
     2881 ggcaacctga agatcgacgc cagcctgtgc ccggatgcca ttgccggcga tggcaacagc
     2941 gaattccgca tcgatccgga catgcaggtg tcgatggggc agtcgctggg caactggacg
     3001 tccagcctgc aggtggcaaa cgatcctgat ccgttcgaag gcgtgatctt caccggtatc
     3061 gccggcacct ggctgctgat gttcattgat gatccctccc ggcgcagtgc gtttgaactg
     3121 ggtgtagtca gtctgctgcc gggtgagcat ctggatcgct accatccgtt cctggccttg
     3181 ctggaatggg tgttgagcga tgtggacccc accctgtata tggatgagat tttccgtaat
     3241 cccaccaaga cggcgccgca catcatggcc atttccggca ttgatgatca cggcagttcg
     3301 gaagtggtgc agcgcacgtt cctgacggcc ctgggtctgg atctggccgg tgaggaactt
     3361 ggcaccacgc cggagaacaa tctgctggcc tacctgcaga aaaccggcac gcaacagttg
     3421 ccgtatccgg tggtgaacaa tatcagtgta cccggccagg gccagcgtac cggcgcgatc
     3481 gtgcgttatg aaaataccgt gcgccccgat cacaacggcc accatgtcac cttcgacatt
     3541 cccgcaccca aacaccagta cggctgcttc atgcagaatc tggcggaagg ccgggcgccg
     3601 gtgatcgtgg aaggcagtgt gcagaacggt ccctgcctgt aaggatgttg gaatagcgct
     3661 gcaggcgcgg ttgttgttct gacattgcca agcgttaccg gacggtgcct tgtttccaat
     3721 catggcgccg cccaccccgc aggtgcgcac agcgtacagt tatgctgttg cgcctgcgtt
     3781 atcacggagt cgtttgtcgt caccgttttt catcgcaagg attgccgatg ttgactgtca
     3841 actgtttgcg ctgttcgcgc tttcttgtct gtgtcttgct gctactgatc gccggctgca
     3901 aacagaccat cgaaatgcgg atggatgtga atgcggtgcc ggcagcgcgc tcgaaacagt
     3961 tcttccacaa cggatttccc accgacctgc gtcggcagga ggatggcagc atcagcctga
     4021 tcgacattcc gcacggccag gaatatttca cgcgtttata cgccgacagc attcagcagg
     4081 aagccattgg ctactcgccg gtgatgccgg tgtacctgcc cttcagtggc gcgttgcagc
     4141 tggatttgct gtcatcgaac gaaacggatt ttgccagtgc cgatgcgccg atccagctga
     4201 tcgacatcga tgcggattcg cctgaatacg gccgccgttt tccgttgcag ctctcgatga
     4261 cctggcaggc ggacagctac cgccccagtc gtctgctgca ggcgttgccg tcgcccgggg
     4321 tgaatttgcg cacgcacacc acttatgcgc tgattgtcac cgatgcggtg cccgtttccg
     4381 cggaggaccg gttccggcag catccgcagc tgcagaccct gctcgatccg gattcgaacg
     4441 cggcggatgt cagtgatcgc gcgctggcag tgtttgcgcc gctgcggaat tttctggcaa
     4501 cggaaaacat cgacgctgcc cgcatcatgg gcgccaccgt gtggaccaca ggcgatccca
     4561 cggcgtggtt gcgccggggc gcgcaacaca tgacgacact gccggtgccg gcaccgcaaa
     4621 atgtcgtgcg cgccgaggat tttcccgact actgcgtgat ccgcggcacc tttgccgtac
     4681 ccggtttcca gcgtggcacg attccatatc cgctggtcaa ttacggcggc gacatccagt
     4741 ggaatgagga cggcgcgccc attcagcagt acacgcgcga agcgaaattc gtggtgacga
     4801 ttccgaagca cgccagcatg cctgacaacg gttttccggt gatgtggttc aatcacggtg
     4861 ccggtggcac ggcggatcag gtattcaccc gcggtgcgca tattgatatc ggcgtgcggg
     4921 tggaaggcgg cggacctgcg atgatcgcgg cacagaaagg ctgggtcacc gccagcatgg
     4981 cggcgcacat gtcgatggac cacctgggcg tactcaatgg cggtcagggc atctgggaat
     5041 acgatctgat aaatccggtg gcgatgcgca acaactacgc gcagatggtg tgggagaaaa
     5101 tcctgttccg ttattttatc cgccagttgc ggctcgatgc gtcgctgtgt ccggaaaccg
     5161 atcccggtgc caaccgcgtg gaatttttcc tggaccgcga cagggaagtg ttgatgtcgc
     5221 agtcgctggg caactgggtg ggcacgatcc agacagcagt ggaaccggag ccctatatcg
     5281 gcgtgatcta cagcgggttt gccgccagct ggctgcggtt tttcaccgac aatttcgtca
     5341 aggcgcacgc catcgaaacc ggcatgctgg cgttgccgcc gctggaacat ttcgatcagt
     5401 ggcatccgtt catgatgctg ctgcagtggc tggcagcagc ggccgatccg acactgtatg
     5461 cgccggacat tctgcgcgcg cccaccaaaa ctgcgcccca cgtgctggcg atctcgggcc
     5521 agttcgaccg ttccagcagc gagtgggtgc agcaggtcta cgccaccgcg ctgggcgtgg
     5581 atctggcggg gccggaactg ggtgcggaca ataaaacgcg cattctggaa tacatgcagc
     5641 agttcggcgc gcgtcagctc gattatccgg tggcaaacaa tctggacatt cccggccagg
     5701 gcatgcgcac ggcggtggtg gtgcggcatt tcaattccgg cagggcgggc aacaacggac
     5761 acaacgttac ctatgacatc gatccaccca agcaccagta cggctgtttc ctgcagcagc
     5821 tggcagacgg gagggcaccg gtgattgtgg aaggcagcgt gcagaacggg ccctgcgatt
     5881 gatctccggc ggtatttggc gcggcagctt tgctatgctt gtctcaccac agtatggact
     5941 ccgggtgcac tcgcatggct tcaccgaaga tggataccgc cgccgatccg gcccagggtg
     6001 ccagcccaac cccgcctgac gtgacagcgc ctgctggaac ggaaccggat cgtcctctca
     6061 tcctgattat tgatgacagc cccgaaagca tccgcctgct gagcgggctc attctcgaca
     6121 agggcgacgt cgtgtttgcc accagcggcg aggccggtct gaccctggcg cgtcagcgcc
     6181 gcccggatct gattctgctg gatgtggaaa tgcccgacct gtcgggctac gaggtgtgtc
     6241 gccggttgaa ggatgatgcg gatacccgcg acattgctgt gatgatcgtc accgcacatc
     6301 attccagcgc ccatgaagtg caggcactgg aagccggtgc gatcgatttt ctgaccaagc
     6361 cgctggcacc ggccgtggtg cgcgcacggg tgcaaaccca gctcaccctg aagcgtcagg
     6421 ccgatgccct gcaccggcag gccatgctcg acggccttac cggcctctat aaccgcgcct
     6481 attttcacga acatctggaa ctggaatggc gacgccacct gcgccagcag gctccgctcg
     6541 gcttcgcgtt gctggatatc gattatttca aggcattcaa tgaccagtat ggtcatgtcg
     6601 ctggcgatgc ctgtctgcgc cgggtagcgg cagcgctggt ggccagcacg cgccggccgg
     6661 cagagttcgt ggtgcgttac ggcggggagg aattcgccat cgtgatgccc aatacgccgc
     6721 gagaggagat tggccggttt ggtgagtgga tctgccgcca ggtgcaggat ctggcgattc
     6781 cccacagtgc ttcgtctgtt gcaccggtgg ttacgttgag tgccgggctg gtggtgctgg
     6841 tgccgggtac ggccgtgacc tggtcggacc tgatcgagcg ggccgaccgt gccctgtacc
     6901 gggccaagca ggaggggcgc aaccgggcag tggttgccga ctgatgctgc gcgccgtttc
     6961 gctgtttgcc gttatcaccc tgctggcgct gccctgcatc atgctgacgc gggaggccgg
     7021 caatatcgcc tatttgtggt tgcccaatgt ggtgggcttt gtggtgctgc tgcgtgccgg
     7081 tacgcgccag tggcccttct tgctgctggc aatcctggca ggaattctgt cggcggggct
     7141 gttctatggc gacgccttca gtttcgccct cggtacaacc ctggccaatg tcctggaaat
     7201 cctgctggcg gcgacgctgg tgcggcgctt cgtgctgcgg cccggtgata tgcccggcat
     7261 ccagaatgcg ctgcagttgc tgggtctgta tgtattggtg gcggccccgc tgggggccct
     7321 gctgggtgca ctggtggtgc gggccagttt tggcacgccg ctgccggagg taatcggcaa
     7381 ctggtggctg ggggatatga tcggcgtgct gctgctgggc ctgccgggtt tgctcctgcg
     7441 ccgtgagcac ctgcgctacc tgcgccagcc gcggcaggtg gccgatttcc tgctgacctt
     7501 cgcgattctg gcgctggtgt cctacctgtt gctgcgttat gtccgcttcc ccttcg
//
LOCUS       DASUEG010000196         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1772868, whole genome shotgun sequence.
ACCESSION   DASUEG010000196 DASUEG010000000
VERSION     DASUEG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772868"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1946)
                     /locus_tag="VFX11_03715"
     CDS             complement(180..1946)
                     /locus_tag="VFX11_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4937755.1"
                     /translation="MPSIRIDVRWVLDGLLEDARITERDLNFVTGQRREKTELNWNPL
                     QFVAKFNLTDQHDEHHKLTLDKLTDWLSKRANQPVYHIDPLKVDVTATTSVMSFNFAE
                     RHEILAVAVDKDSVTIASGQPFLSDWEHGLEQTLRGKKIKRVIVNPADLRRYTLEFYN
                     LARSVKGATGKDAGQSHVANFEQLLQLGELGSPDANDQHIVKIVDWLLQYAFDQRASD
                     IHLEPRREHGYIRFRIDGVLHDVYELPAAVMAAVTSRIKIIGRMDVAEKRRPQDGRLK
                     TKSGEGDEVELRLSTLPTAFGEKMVMRIFDPEVLVRSAAQMGLTDEDYEKWQTMVNQP
                     NGIVLVTGPTGSGKTTTLYSTLKQLATREVNVCTIEDPIEMVEPAFNQMQVMDNIDLT
                     FADGVRALLRQDPDIIMVGEIRDLETANMAIQAALTGHLVISTLHTNDAPSAVVRLMD
                     LGVAPYLISATLLGVMAQRLVRTLCPHCKRDTHIDPEAWDALISPFKGKMPERIKMPV
                     GCLECRKTGYLGRVGIYEIMVLSQELKDMIAHSAELNTIRKQALKEGMRTLRLSGAHK
                     VATGLTTPEEVLRVAPATTGLS"
     gene            2191..>2627
                     /locus_tag="VFX11_03720"
     CDS             2191..>2627
                     /locus_tag="VFX11_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEX4937756.1"
                     /translation="MESVIEKILYASRWLMAPVYLGLSVLLLALAIKFFQELIHIFPL
                     LFTMKEQDLILAMLTLIDLTLVGSLIVMVMFSGYENFVSRIDIDTSGDTPAWLSKLDS
                     GTLKLKVAASIVAISSIHLLRIFMNAQQVPNDKILWYVVLHLT"
BASE COUNT          498 a          794 c          782 g          553 t
ORIGIN      
        1 cggatccgtt ggcctgtgtc cgcttatacc cggtgagttt ttgtacaacg cacggccatt
       61 ctaacaagcg ttgatgccgg gttggaacga atggtaatta aaaagataaa caaaaggaca
      121 gtaaaggttc acacattcag ccagacccct gccagatcgg gtcgctgcca cccggcccct
      181 caggacaaac cggtggtggc tggcgccacc cgcagcactt cctccggcgt ggtcaggccg
      241 gtggcgacct tgtgagcccc ggacaggcgc agggtgcgca tgccttcctt cagggcctgc
      301 ttgcggatgg tgttgagttc ggcggagtgg gcgatcatgt ccttcagttc ctgcgacaac
      361 accatgatct catagatccc cacccggccc aggtagcctg tcttgcggca ttccaggcag
      421 cccaccggca tcttgatcct ctccggcatc ttgcccttga agggactgat caatgcgtcc
      481 caggcctcgg ggtcgatgtg ggtgtcacgc ttgcagtgag ggcacagggt gcgcaccagg
      541 cgctgggcca ttacacccag cagggtggca gagatcaggt aaggggccac acccagatcc
      601 atcaatcgca ccacggcgga cggcgcatcg ttggtgtgca gggtggaaat caccaggtgc
      661 ccggtcagcg ccgcctgaat tgccatgttg gcagtctcca gatcgcggat ctcgcccacc
      721 atgatgatgt ccgggtcctg ccgcagcagc gcgcgcacgc catcggcaaa ggtcaggtca
      781 atattgtcca tcacctgcat ctggttgaag gcgggctcca ccatctcgat cggatcttcg
      841 atggtgcaca cattcacttc ccgcgtggcc agttgcttca gggtggaata cagcgtggtg
      901 gtcttgccgg acccggtagg accggtcacc agcacgatgc cattgggctg gttcaccatg
      961 gtctgccatt tttcataatc ctcgtccgtg aggcccatct gggcagccga gcgcaccagc
     1021 acttccggat cgaaaatccg catcaccatc ttttcgccga acgcggtggg cagggtggac
     1081 agacgcagtt ccacctcatc accctcgcca ctcttggtct tcaggcggcc atcctgtggc
     1141 cggcgttttt ccgccacatc catgcggccg atgatcttga tccggctggt gacggcggcc
     1201 atcacggcag cgggcagttc gtaaacatca tgcaacacac catcgatgcg gaagcggata
     1261 tagccgtgtt cgcgacgcgg ttccagatga atgtcgctgg cgcgctggtc gaaggcatat
     1321 tgcagcagcc agtccacgat tttcacgatg tgctggtcgt tggcgtcagg ggaacccagc
     1381 tcccccagct gcagcagctg ttcgaaattg gcgacatggg actggccggc atccttgccg
     1441 gtggcgccct tcaccgaacg cgccagattg tagaattcca gcgtgtaacg gcgcaggtcg
     1501 gcgggattga cgatcacccg cttgatcttt ttgccgcgca gggtctgttc cagcccgtgc
     1561 tcccagtccg acaaaaaagg ctggccgctg gcgatggtga cggaatcctt gtccaccgcc
     1621 accgccagaa tctcgtggcg ctcggcgaaa ttgaacgaca tcaccgaagt ggtggcggtc
     1681 acatccacct tgagcggatc gatgtgatac accggctggt tagcacgctt ggacagccag
     1741 tcggtgagct tgtccagggt cagcttatgg tgttcgtcat gctggtcagt caggttgaat
     1801 ttggcgacga actgcagggg attccagttc agttcggttt tttcccggcg ctgccccgtg
     1861 acgaaattca gatcccgctc ggtgatgcgg gcatcctcca gcaggccgtc gagcacccag
     1921 cgcacatcaa tgcggatgct gggcatgctg aaattggctt tgccgggttt ggcactggac
     1981 acggggggat aactcctgac tgggtgagtg gcagatgcag cacactaaca ggcggcactc
     2041 tgaaaggatt gtagtctcaa gcgcttaagg gctcaaaatc cggcccccgg aatcgtgacc
     2101 cggcccgtaa aatccggtat cgttcgcgca aatcaacctt cccgggttgc ccgttgtcgg
     2161 gcgatcctgg cgcagtctcg agggcctgtc atggaatccg tcattgaaaa aatcctgtat
     2221 gccagccgct ggctgatggc gccggtgtac ctgggcctga gcgtgctgct gctggccctg
     2281 gccatcaagt tcttccagga gctgatccac atcttcccgc tgctgttcac catgaaggaa
     2341 caggacctga tcctggccat gctgacgctg atcgacctga ccctggtggg cagcctgatc
     2401 gtgatggtga tgttctccgg ctacgagaat ttcgtctcgc gcatcgacat cgataccagc
     2461 ggcgacacgc cggcctggct gtcgaagctc gactcgggca cgctgaagct gaaggtcgcg
     2521 gcctcgatcg tcgcgatctc ctcgatccac ctgctgcgca tcttcatgaa cgcgcagcag
     2581 gtgccaaacg acaagatcct ctggtacgtg gtgctgcacc tgacctt
//
LOCUS       DASUEG010000197         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1286032, whole genome shotgun sequence.
ACCESSION   DASUEG010000197 DASUEG010000000
VERSION     DASUEG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286032"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..451)
                     /locus_tag="VFX11_03725"
     CDS             complement(80..451)
                     /locus_tag="VFX11_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX4937757.1"
                     /translation="MNRILTSLLLILCLSACGADGGAQGSDAASRSATPSFADTDAAM
                     EPCAAVASQQPQLESRVVALRELADAGEVCAQYGIGTLYVFGYETIDPDPALAQRYLQ
                     LAAAQGHSAAQRELDQHFSGL"
     gene            complement(448..2469)
                     /locus_tag="VFX11_03730"
     CDS             complement(448..2469)
                     /locus_tag="VFX11_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937758.1"
                     /translation="MKCRRSQRHPFGRNLLLVVAGGLLSISAHAATLAGDCHPARRLT
                     CTLPFPSDLFAEEDAQSPTGKRVIIPNSALALLRNETQNKPVLLPESVRPEGLFQDAS
                     GFSAGAPVLFELDRAVNESTLPFDGGLALLVFEIGGDDVPVPVNVRVMEVARGQRAAR
                     KDVVIEAWPRARFTFGKSYVAALTRAVKPLMGTDFAPAPVLAGILAGQPSPLTAAYAP
                     ALTTLDARLASRGLTRADILDLTFFTVRDEAEVVQPIGRFMQRAYADAHPLKRLSVEY
                     FPYQTIAAVVTGLVRLTDFRAADGNLVRDPDYPGTENWVEFRFAIPRDVRLASAPVVI
                     YGHGLASKKEDAGYTEKLNALRNIATLSIDQPNHGYRGEFEGGGLFDILQPQDLGRVL
                     GMVIQSSIDQVSLLAALEHEFRQLDVYPRNAKANVFPWLALRGQDQQNDLNLERIFYK
                     GTSMGGVLGSAFIGAAPRLDGAFIHVPGVGVANIVSHSVVWALFGRAVPNGTVGAEIA
                     AYLGMVQQGLDYGDGLNFAGYYRTGTDLLPYPVNPKPVSIEYGVGDGVVFNDSSVAMM
                     EIAGMPLAHPHNPAIADHYDASPVPKLPDDVWFDDGYGAMPLESPRDPDARDPWVINL
                     VSPYLNGWSTGSLAAHGAFRTDAGAAYEGAWTDTFIQKNPRDRQPESAP"
     gene            2738..4078
                     /locus_tag="VFX11_03735"
     CDS             2738..4078
                     /locus_tag="VFX11_03735"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX4937759.1"
                     /translation="MANDKKDDKRFKATTPAKRFMKLAGMSASITKNLTTHKLKGLFA
                     DEETQQKNQEKLYADIGQQIAQTLGEMKGAVMKVGQIASQVKDMLPKEVASALEVLQK
                     ESPPMPFPMIRRQLIKALGDTPENLFARFDEVPFAAASIGQVHRALTKEGVECVVKIQ
                     YPGVKESCDSDLKHLRRLLKLAGLVKVSAEVMDETFAEIRTMLYEELDYKHEAENLKL
                     FGDYYRNHPYIRLPELIENLSAETVLTLTLVEGDRLQDVKPPRYSQETINLIGYRIFE
                     TFGKQIYELRAVHSDPHPGNFAFRPDGTIIIYDFGAVKKIPEATIAKLRSLVADAMAG
                     RIDTIDQHMLDIGVRQPNGEAPSPEYYHEWMDIVMQPFQSYESFDFAHTNLHRRALGK
                     VKRDGLKYMGSFQPSPETLAVNRVLTGHYWTMVDLGVKASFRPLIDEIVLKKTA"
BASE COUNT          875 a         1255 c         1247 g          706 t
ORIGIN      
        1 cgcgcattat atcgaaagaa ctgcgcgtgc acagtaacgg cgcaacggat tttgacgaac
       61 agcccgaaac ggtgctggtt tataaaccgg aaaagtgctg atccagttca cgctgtgcag
      121 cactgtgccc ctgcgccgcc gccagttgca ggtaacgttg tgccagtgcc ggatcgggat
      181 cgatggtttc ataaccgaac acatacagcg taccgatacc gtactgggcg caaacctcgc
      241 cggcatcggc cagttcacgc agcgccacta cccgcgattc cagttgcggt tgctgactgg
      301 ccacggccgc gcagggttcc atcgcagcgt cggtatcggc aaaagacggg gtggcgctgc
      361 ggctggctgc gtcactgccc tgggctccgc cgtcggcgcc gcaggcggac aggcacagga
      421 tcagcaacag gctggtcaga atacggttca tggtgcgctc tccggctggc ggtcgcgggg
      481 atttttctgg atgaaggtgt cggtccaggc gccttcgtag gcggctccgg catcggtacg
      541 gaaggcaccg tgagcggcca ggctgccggt ggaccagcca ttgagataag gcgataccag
      601 gttgattacc cagggatcgc gggcgtcggg atcgcggggg gattccagcg gcatggcgcc
      661 gtagccgtcg tcgaaccaga catcgtccgg cagcttgggt actggcgatg cgtcgtagtg
      721 gtcggcgatc gccgggttgt ggggatgggc cagcggcatg ccggcgattt ccatcatggc
      781 cacgctgctg tcgttgaata ccacgccgtc acccacgccg tattcgatgc tcaccggttt
      841 gggattcacc gggtacggca gcagatcggt gccagtgcga tagtagcccg cgaaattgag
      901 gccatccccg taatccagtc cctgttgcac catgcccaga tacgcagcaa tttctgctcc
      961 cacagtgcca ttcggcacgg cacgtccgaa cagggcccag accacggaat ggctgacgat
     1021 gttcgccacc cccacaccgg ggacatgaat gaaggcgccg tccagccggg gtgccgcacc
     1081 gataaaggcc gaccccagca cgccacccat cgacgtaccc ttgtagaaga tgcgctccag
     1141 attcaggtcg ttttgctgat cctgcccacg cagggccagc cagggaaaaa cgttggcctt
     1201 tgcattgcgc ggatacacat ccagctgacg aaattcatgc tccagcgccg ccagcagcga
     1261 cacctgatcg atgctggact ggatcaccat gcccagcacc cggcccagat cctgcggctg
     1321 caggatgtca aacaggccgc cgccctcgaa ttcaccccgg taaccgtgat tgggctggtc
     1381 gatggacagg gtggcaatgt tgcgcagggc gttgagcttt tcggtgtagc cggcgtcttc
     1441 cttcttgctg gccagtccgt gcccgtaaat caccaccggc gccgacgcca gccgcacgtc
     1501 gcggggaatg gcgaagcgga attccaccca gttttcggtg ccggggtaat ccgggtcgcg
     1561 taccagattg ccgtccgcgg cgcgaaaatc cgtcagccgc accagcccgg tgacgaccgc
     1621 tgcgatggtc tgatagggaa agtattccac cgacaggcgt ttcagcggat gggcatcggc
     1681 ataggcgcgc tgcatgaaac ggccgatggg ctgaaccacc tcggcctcgt cgcgcacagt
     1741 gaaaaaggtg aggtcgagaa tatcggcgcg ggtcaggcca cggctggcca aacgggcgtc
     1801 cagcgtggtc agcgcgggcg cataggccgc cgtcagcggc gatggctgcc cggccagaat
     1861 accggccagc accggagccg gagcgaagtc ggtacccatc aagggtttaa cggctcgcgt
     1921 cagggccgcc acataggatt tgccgaacgt gaaacgcgcg cggggccagg cttcgatcac
     1981 cacgtccttg cgcgcagcac gctggccccg ggccacttcc atcactcgca cattcacggg
     2041 aaccggaaca tcgtcgccgc cgatttcaaa caccagcaag gccaacccgc cgtcaaaagg
     2101 cagcgtggat tcattcacgg cccggtccag ttcgaacagc accggcgcac cggcggaaaa
     2161 accgctcgca tcctggaaca ggccctccgg gcgcaccgat tccggcagca gcaccggctt
     2221 gttctgggtt tcgttgcgta gcagcgccag ggcggaattg ggaatgatca cccgcttgcc
     2281 ggtgggcgat tgggcgtctt cctcggcaaa cagatcgctg gggaacggca gggtacaggt
     2341 cagacggcgg gccggatggc agtcgcccgc cagggtggcc gcgtgcgcgg atatgctgag
     2401 cagtccgccc gccaccacca gcagcagatt gcgcccgaac gggtggcgtt gagaacggcg
     2461 acatttcatg ggattcccct gtttattctt gtttttgatg gaggcgcccg ccgacaccac
     2521 gccggcgtcc ggaaccgtcc acgggcccgt cattctatag cgccccgaca aaaccaggca
     2581 acaaaatggt accaacgtgc acaatttaag cgctcaggcc cctgccattt acgccagcca
     2641 gtgaacggat cgcgtactag ccactccaac gtcatgctat gtttattgac agtatccggt
     2701 tgactgacag acccgcccca ccacgcagag gtaccccgtg gcgaacgaca agaaggacga
     2761 caagcgcttc aaggccacca cccccgccaa gcgcttcatg aaactggcgg gcatgtccgc
     2821 cagcatcacc aagaacctca ccacccacaa gctcaagggc ctgtttgccg acgaggaaac
     2881 ccagcagaag aaccaggaaa agctgtacgc cgacatcggc cagcagatcg cccagacgct
     2941 gggggaaatg aaaggcgccg taatgaaggt ggggcagatc gcctcgcagg tgaaggacat
     3001 gctgcccaag gaagtggcca gtgcgctgga agtgctgcag aaagaatccc cgcccatgcc
     3061 cttccccatg atccgccgtc agctgatcaa ggccctgggt gacacgccgg aaaacctgtt
     3121 cgcccgcttc gatgaagttc cgtttgctgc cgcgtccatt ggccaggtgc accgcgcgct
     3181 caccaaggaa ggcgtcgagt gcgtggtgaa gatccagtat cccggcgtga aggaatcctg
     3241 cgactccgat ctgaaacacc tgagacgcct gctgaaactg gcggggctgg tgaaagtcag
     3301 cgccgaggtg atggatgaaa ccttcgccga aatccgcacc atgctgtacg aggaactgga
     3361 ttacaaacac gaagcggaaa acctgaaact gttcggcgat tactaccgca accaccccta
     3421 tatccgactg ccggaactga ttgaaaacct gtcggcggaa acagtgctga cactgacact
     3481 ggtggaaggc gaccgcctgc aggacgtcaa gccaccgcgc tattcgcagg agacgatcaa
     3541 tctgatcggg taccgcattt tcgaaacgtt cggcaagcag atctatgaac tgcgtgccgt
     3601 gcattccgat ccgcacccgg gaaattttgc gtttcgcccg gacggcacga tcatcattta
     3661 cgatttcggt gcggtgaaaa aaattcccga ggcaaccatt gccaaactgc gcagcctggt
     3721 ggcagatgcg atggccggca ggatcgacac catcgaccag cacatgctcg acatcggcgt
     3781 gcgccagccc aacggcgagg caccatcgcc ggagtactat cacgagtgga tggatatcgt
     3841 gatgcagccg ttccagagtt atgaaagttt cgatttcgcc cacaccaacc tgcatcgccg
     3901 cgcgcttggc aaggttaagc gggatgggtt gaaatacatg ggatcattcc agccgtcgcc
     3961 ggaaaccctg gcggtgaacc gggtgctgac cggacactac tggacgatgg tggacctggg
     4021 cgtgaaagcg tcgttccgtc cgctgatcga tgaaattgtg ttgaagaaga cggcgtaaaa
     4081 aca
//
LOCUS       DASUEG010000198         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1342623, whole genome shotgun sequence.
ACCESSION   DASUEG010000198 DASUEG010000000
VERSION     DASUEG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342623"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFX11_03740"
     CDS             <1..599
                     /locus_tag="VFX11_03740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4937760.1"
                     /translation="TLEYQVRLGFLQRGALKNLAKTDPLTGLYNRGAINQKLNHLIYY
                     AFRERKPITLLLVDVDFFKNYNDFYGHIQGDNCLIRLAGALASCCKRPLDFAGRYGGE
                     EFLVMWFDAEPNEAEAFARRVRESIRELGIRHDASGVNSDVTISGGMVTGVPDSQHLT
                     EKLLHQADQCLYRAKESGRNRIIVQDFGDDKSIAHIIK"
     gene            760..2766
                     /locus_tag="VFX11_03745"
     CDS             760..2766
                     /locus_tag="VFX11_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4937761.1"
                     /translation="MTKRVLNPSAWKLALCLILAWCCTSVASAAETIALDDNSDQLNL
                     VHHVEYLVDDSGKLTVNDVQDSTRDREWQRPVNVIDNVNFGYTSASYWFRFRLHNDTT
                     HTIAKQLEIGYPVLDYIDVYQQFSNGNSVNLSMGDKRPFQDRPVNHRNFIMPLKLQPQ
                     DMVDIYIRVHTTSSMQVPLMLWRDTTLLEAAQAEMLGLGIYYGTMVVMILYNLFVFLS
                     VREANYLYYVLYVACMALFLASLNGVSYQYLWPTGIWWNDQSIVFFLAGVVLFAALFT
                     INFLRIHEVFPRLRIAANGVVVISAGVMLATLFLPYSTMISVVIAWAVAGISLAILFG
                     SYRWLNGDSSAKYYCIAWYTMLFGGVILALNKFDMVPRNFFTENATQFGSALEVILLS
                     FALADRLNVEKRRRFEAQLQALEHERVARLAQAEALQQEKNARLAQEKALLHEREARN
                     AQDKALEIQMRANETLEYKVKERTHELEIANKRLEELTYTDGLTGIRNRRYLNRALER
                     EFSRAKREKLPLSLLLIDIDHFKQFNDKYGHLVGDDCLRTVATTIEDSALRENDIVAR
                     YGGEEFCVLLPNTDQPGAHQVAELIRQRVQQADFQVGGTAVTITVSVGLTTCIPERHH
                     EIDQFVAAADSALYSSKRNGRNQVTAELPSFGPNLQPAPGPAAS"
     gene            2763..>3539
                     /locus_tag="VFX11_03750"
     CDS             2763..>3539
                     /locus_tag="VFX11_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4937762.1"
                     /translation="MMRMLQRLLPPLVLLLASFTSLCSQASTGLVLDSSVTNLRLNGQ
                     LQFFEDKSNRLRVEDIAQQLPAFHIYEKDSVNEGYSSSAWWFSFQLYNNEQMPIHWWL
                     DISYPLLDEVELYELDPATGSIRSIALTGDTRPLSSRSAKTGTFVFPLQLQPQREYRY
                     LLRVWTTGTIAVPMELHSSTAFVGYYGDQRLLTGIYHGGALFLMLYNIGLLVAVRERL
                     YLYYVMYVACFLITTFTATGVGFEFLWRDQYWFQSGAMPVT"
BASE COUNT          765 a         1057 c          959 g          758 t
ORIGIN      
        1 acacgctgga ataccaggta cggctgggct tcctgcagcg cggtgcgctg aagaacctgg
       61 ccaagaccga tccgttgacc gggctgtaca accgcggcgc catcaaccag aaactcaacc
      121 atctgattta ttacgcgttc cgcgaacgca agcccatcac gctgctgctg gtggatgtgg
      181 atttcttcaa gaactacaac gacttctatg gacatatcca gggcgataac tgtctgatcc
      241 ggcttgccgg cgcactggca agttgctgca agcgaccgct ggattttgcc ggtcgctacg
      301 gcggtgagga atttctggtg atgtggttcg atgctgaacc caacgaagcc gaagccttcg
      361 cccgccgcgt gcgggaatcc atccgggaat tggggattcg tcatgatgct tcgggcgtca
      421 atagtgacgt gaccatatcc ggtggcatgg taacgggcgt gcctgacagc cagcatctca
      481 ccgaaaagct gctgcaccag gccgaccagt gcctgtaccg cgccaaggaa tccggacgca
      541 accgcatcat cgtccaggat ttcggcgacg acaaaagcat cgcgcacatc attaaataaa
      601 cgtacgccca ttcggtttca aataacccgg ccctttaaca ttgcgcctct tgattccatt
      661 tggttctttc aacgaaagaa cggcgccttt tgccatcact gactaaactg aataaataag
      721 gggtaacccg cagcaaagcc gtggcgagag aaaaaccgag tgacaaaacg tgttctgaac
      781 ccgagcgcat ggaagctggc actctgcctg atactggcat ggtgctgcac cagcgtggca
      841 tccgccgctg aaaccattgc actggatgac aacagcgacc agctcaacct ggtgcatcat
      901 gtcgaatacc tcgtggatga cagcggcaaa ctcactgtca acgacgtgca ggactccacg
      961 cgggaccgcg aatggcagcg tcctgtcaac gtcatcgaca atgtcaactt cggttacacc
     1021 agcgccagct actggttccg cttccgcctg cacaacgaca ccacgcacac catcgccaag
     1081 caattggaaa tcggttaccc ggtgctggat tacatcgatg tctatcagca gttcagcaac
     1141 ggcaacagcg tcaatctgag catgggtgac aaacggccgt ttcaggatcg cccggtcaac
     1201 caccgcaatt tcatcatgcc cctgaaactg cagccgcagg acatggtgga tatttatatt
     1261 cgcgtgcaca ccaccagttc gatgcaggta ccgctgatgc tctggcgcga taccactctg
     1321 ctggaagcgg cacaggccga gatgctgggg ctgggcatct actacggcac catggtggtc
     1381 atgatcctgt acaacctgtt cgtattcctg tcggtgcgcg aagccaacta tctctattac
     1441 gtgctgtatg tcgcctgcat ggcgctgttc ctggccagcc tgaatggcgt ttcctatcag
     1501 tacctgtggc ccaccggcat ctggtggaac gatcagagca ttgtattttt tctggctggt
     1561 gtggtgctgt ttgccgcgtt gttcaccatc aatttcctgc gcattcacga agtgttcccg
     1621 cgcctgcgga ttgccgccaa cggggttgtc gtgatcagtg ccggcgtgat gctggccacc
     1681 ttgttcctgc catattccac catgatatcg gtcgtgatcg cctgggccgt ggccggcatc
     1741 tccctggcca tcctgttcgg ttcataccgc tggctgaatg gcgattcatc cgccaagtat
     1801 tattgcatcg cctggtacac catgctgttt ggtggtgtga ttctggcgct caacaaattc
     1861 gacatggtgc cgcgcaattt ctttaccgaa aacgccaccc agttcggttc ggcgctggaa
     1921 gtgatcctgc tgtcgtttgc gctggctgac cggctcaatg tggagaagcg ccgccgtttc
     1981 gaagcgcaac tgcaggccct ggagcatgag cgcgttgccc gcctggccca ggccgaagca
     2041 ctgcaacagg aaaaaaatgc gcgcctggca caggaaaagg cattgctgca tgagcgcgag
     2101 gcccgcaacg cccaggacaa ggcgctggaa atccagatgc gcgccaacga aaccctggag
     2161 tacaaggtca aggaacgcac tcatgagctg gaaattgcca acaagcggct ggaagagctg
     2221 acctataccg acggtctgac cggcattcgc aaccggcgct acctgaaccg cgcactggaa
     2281 cgggaattct cccgcgccaa acgggaaaag ctgccactgt cgctgctgct gatcgatatc
     2341 gatcacttca agcagttcaa tgacaagtac ggtcatctgg tgggtgatga ttgcctgcgc
     2401 acggtcgcca ccaccatcga agattccgcc ctgcgggaaa acgatattgt cgcgcgctat
     2461 ggtggcgagg aattctgcgt gttgctgccc aacacagatc aacctggcgc ccatcaggtg
     2521 gcagaactga tccggcagcg ggttcaacag gcggattttc aggtgggcgg cacagcagtc
     2581 accatcaccg tcagcgttgg actcaccacc tgcattcccg aacgccacca cgaaattgac
     2641 cagttcgtcg ccgctgcgga cagcgcgctt tactcatcaa aacgcaacgg gcgcaatcag
     2701 gtgaccgctg aactgccctc attcggcccc aacctgcaac cggcacccgg tcctgccgcc
     2761 tcatgatgcg aatgctgcag cgcttacttc cgcctctggt gctgctgctt gcttccttca
     2821 ccagcctttg cagccaggcc agcaccggcc tggtgctgga cagctcggtt accaatctgc
     2881 gcctgaacgg ccagctgcaa tttttcgagg acaaaagcaa tcgcctgcgg gtggaagaca
     2941 tcgcgcaaca attgccggct tttcacattt atgaaaagga ttcggtgaac gaaggctatt
     3001 ccagcagtgc ctggtggttc agtttccagc tttacaacaa cgaacagatg ccgattcact
     3061 ggtggctgga tatcagttac ccgctgctcg acgaagttga actgtatgaa ctggatccgg
     3121 ccaccggcag cattcgcagt atcgcactga ccggcgatac ccgccccctt tccagccgca
     3181 gcgccaaaac cggcactttt gtgtttccac tgcagcttca accgcagcgg gaataccgtt
     3241 acctgctgcg tgtgtggacc acgggcacga ttgccgtgcc gatggaattg cacagcagca
     3301 ccgcgttcgt cggatactat ggagaccagc gcctgctgac cggcatttac cacggcggcg
     3361 cgctgtttct gatgctgtac aacatcggac tgctggtagc ggtacgcgag cgcctgtatc
     3421 tgtattacgt gatgtacgtg gcctgcttcc tcatcactac ctttaccgca accggcgttg
     3481 gctttgagtt tctctggcgc gaccagtact ggtttcagtc cggcgccatg ccggtcact
//
LOCUS       DASUEG010000199        10924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_117503, whole genome shotgun sequence.
ACCESSION   DASUEG010000199 DASUEG010000000
VERSION     DASUEG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117503"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VFX11_03755"
     CDS             complement(<1..1133)
                     /locus_tag="VFX11_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937763.1"
                     /translation="MKNERFQVFLDYIWRWRAAIFSVLALSSFYGVIISLVHGDGAWI
                     YVDHVAETNVYWGDDAYRWFLARSAWIKPDIYWFNFSLPAWVFLDGVVATLSQGDLLS
                     ARYIKAMLSAFSVFLVYRTCLGLQLARWSALGAALLLAVMPLYFFVGMSFYGESWLLV
                     LVAASAFCHVYGHKRLFLLATAVMPLVRPEGIFFVLVFSAIFLYQRKWRELAALLSFG
                     FVFFISIFIFSDIDSFLGWRVEAAKIYQKQGMSYGWTPISAGFFDIFPAPLFVFSLIG
                     MFMPKGRSLRGFYFGVALVVGHWIYTVSGNRGLIEPRYFVATMPVFMLAFAVFLDGLP
                     AVSWLGRWHGRAWQAVAATLAVAVLLCHLYSLTVVRMAVVAGLR"
     gene            complement(1159..2019)
                     /locus_tag="VFX11_03760"
     CDS             complement(1159..2019)
                     /locus_tag="VFX11_03760"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4937764.1"
                     /translation="MNEGMGQKRIFDYERWGAQLAGLKSRYQGNAPFPHLVLEDFLDK
                     DSLEGALADFEALDNSDAWINYVHFNENKMGMSKIDALPPGLQGVIRHLNSPDFVRFL
                     SALSGIESLKADESCEGGGIHQSTRGGFLNIHADFTVHPHHRNWRRRMNVIVYLGKDW
                     QDEWGGHLELWNRGMTTCEVRVAPLFNRCVIFNTDANSFHGHPEPMNCPDGCFRRSIA
                     LYYYTEEESPLKRATNYRARPDDGVKKVLIRIDSLMVSIYTVIKSFLGSNDNFASQFL
                     KAIQKIVRKI"
     gene            2119..3819
                     /locus_tag="VFX11_03765"
     CDS             2119..3819
                     /locus_tag="VFX11_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4937765.1"
                     /translation="MLQAQLQIRRCKVMLATLRPYAIVISLLIIAVLAIYGQTATFGY
                     VSYDDPGYTLQNHVVNAGLTLDGIKWALTSTTYMSNWQPLVWISYMLDVSLFGAYRPG
                     IHHLVNLGLHLTSSIILFLVLTRMTRQLWPSAFVAALFAVHPLHVESVAWIAERKDVL
                     SGLFWMLTMAAYVFYSERPSIGRYLLVFISMACGLATKPMLVTLPFVLLLMDYWPLRR
                     GLQLPASAAAPSPDAPPLRLILAEKLPLLALSLISSLITYLAQRQAEAMSFGSSLTVA
                     QRVSSAIISYAEYILKTIWPTNLAVIYPYNESWATLQVVMTASALLFVTLIVVLQARR
                     FPYLPVGWFWFLGTLIPVIGLVQVGIQGMADRFTYIPHIGLFIAVVFLLADLGQRHVR
                     IRQASTILGGVAVIILTAIAAVQTSYWRSSEDLFRQAVRVPGNYVAETNLAAILLDKP
                     SRTQDELIEAFARARYAVTLKNNHADAHWILGVALANMGKTDEAILQYLEAIRYNADH
                     GTAWAYLAAIYAQNKLMPKAAAALNEAERIAARQAQLPPRANYRPEGDPDGHLLLRER
                     "
     gene            3889..4632
                     /locus_tag="VFX11_03770"
     CDS             3889..4632
                     /locus_tag="VFX11_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4937766.1"
                     /translation="MYQGKKIIVVMPAYNAARTLRRTHDEIMAQGIVDLVIVVDDASH
                     DETIALAQTLPNTLVHRHAVNRGYGANQKTCYRLALENGADIVIMIHPDYQYTPLLIP
                     AMASMVANLYPCVLGSRILGGYALTGGMPLWKYIANRFLTFVENLLTGAKLSEYHTGY
                     RAFSRELLMRLPLEENSDGFIFDNEVLAQILWTGTVIAEVSCPTRYDAESSSINFTQS
                     IKYGFGCLAVGLRYRLARWGLKSKLFPRL"
     gene            complement(4664..6874)
                     /locus_tag="VFX11_03775"
     CDS             complement(4664..6874)
                     /locus_tag="VFX11_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937767.1"
                     /translation="MNLNKKENNRVLLGAAISMGLTALAVDASALQALPDEEMSSVVA
                     KAGITLKMNEPAAGITASSAALLTNNPTPWTPNEANELGVRLSTINIKRATGVTSPFF
                     TATLDFGVPTASSDEFVALSAGLARSQIDIGAVNLAGTTSGVGSGSLGHYLIETPIDL
                     SLVGRNLFDSNTASYGSSLSLNFGKVNKAGWAPGYQGATDPGKIFWRRADDVAGTSPE
                     MVMNDFSFLFELADGRFGFKNNSFVIQSKTGSKVGFSLSLDMLYDGSGSSAFAIGATD
                     KPMLFWGWRGHWTDLNLEFLKNGGRVDGSGRGINSSIKFNYDPDFVWAVGEGGDKPGY
                     LEFGNWTKLPNNTYAFDIPFFSIEALAGGATAQGKGGLCWGNAAITATANNACSGAGV
                     GPVASLDAVQPIKVRRDTDPSVLAITFRDMALSAYASTVKVVDRTLTGKAGVPVVTTY
                     NWALIYTLADLDAELFVSPYADGQSARLDLAVMSQTHAADANSTDRTRWNTGTNFMIG
                     DSDTDFGIGLMGADVLIALRNGRMGMTSTGIKTDAPLFRYQLRGMLGGGAIPDMTIPQ
                     QMAYVDVNVEADRYVFTIGPGQGVTDSYLAYSGFINLANLSEADFASPSGTGNHAHDD
                     GSYLSFAEPSFSKRGIDVRFADVRGSVGVGQGRIEMQGEGDLGAGKIQLLVSNNLYVG
                     LSSSVPCAWGVSCTEAAGNPLVVNTLEFGGKSLGSLAMPSGIVRSQVTIFPKIP"
     gene            complement(6947..8530)
                     /locus_tag="VFX11_03780"
     CDS             complement(6947..8530)
                     /locus_tag="VFX11_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937768.1"
                     /translation="MKVERIVGLLMTAFMRASLKWQPGLVVFRGRGRADARKWRISQR
                     GAVKAMSLLMGLVVGLMLLPAINQAGTQRLEEKGLVAAGARPAPVPAGLTAMDDSEMA
                     QVNGQALFMSSHERNVLGTTTYDFYKMGLDAQLDLNVNIAELKLGEDTSGVDLWIKNL
                     AMGCIGATASPTTADCIVAGSGTTETGKVLKPFTLLRPFVQIAVKGDNAATREVVGIR
                     LGAQNAQGPFSAGNFNSFSGYMSGTAALQMQGQGNDGRVGWYSNGGNPSGCTNNMCTG
                     VALTNDSDFRVANTRWDHLNQSQLSCASTSVTNTGCAGGYNFGFNNQYVDAGITGANT
                     RTLLVGFNTVNANGNVVASGSRLSQALVSGLNLANVVGTLGDSVSVKASTSLGSGLLN
                     TFLPFLIGQVKTDLKNQLATGLGITYTGNVSDAALNGYKMPFNLENFHSTMVDSSLFG
                     LSFQKESVQYPGYAVAMQKGWSMYLPNAFALNIAGPTTLLVSNIVSSNSDTAGNITGL
                     PANAGGRVYDNCWGTSVFC"
     gene            complement(8533..9312)
                     /locus_tag="VFX11_03785"
     CDS             complement(8533..9312)
                     /locus_tag="VFX11_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937769.1"
                     /translation="MKLLSGFVMALVAFPVMAADLRPLDDGELAGVSGGDGIAIGWEY
                     GVNTDTSGAPLATLGSCAGSGTVTATGGDRCRFAWQVAKRTGNGNAAWTVAKNGWLSV
                     KIPALNIDVQATMGGAGGVGANNTYFDNDRFMGYDNLGASVCLLPGGGASCAYATIDT
                     LPAIKLYYPVASYNATTYTPGTGVSTGFTSLQIGLNIGRMSIEYDTCTALTNCTSASP
                     GYVPGYLRDANAGSFLGTQIRDNNGNFAMMAIGGSALIYGF"
     gene            complement(9316..>10924)
                     /locus_tag="VFX11_03790"
     CDS             complement(9316..>10924)
                     /locus_tag="VFX11_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937770.1"
                     /translation="VGSCLLVVSLAMGTQAHALESLDDGALAEVNGTGVALAFDNFSS
                     RFAPTSYINMLGTNVVDNTGIVFDATLNGGEGGCRNSASGSPALPASSCDPYDAPSNW
                     RRGDLYYYGLSMSGGNVNYGMDWYGTGTAGGGCTPGADGLGCPIGRINGSSQVEGIKN
                     WASVYNPYMIRVFQYAGYSYEGTCLGSEVSGKCSQVSLATSPTYYEFVGPSKGDKWRW
                     AFWGELLVNNGALALGGAGDTFSGTCNGASVTAGANCAGILKSQTIILGNNFTLDNKP
                     TRLQIVQTPTTTVSEQSLSVVYQSRLSGNFRFSVAQTGTYAQSVAHGIVPDFNDVEGM
                     VFKNVDAFLPLGQNNYQSVVFRNNGTSGNFTVELTPISSNTNVSNTFYCGQNNCASFV
                     TSGIPEATGGVGLNGSNAAIGCVNQLGNVVACTGIYDRSVKVKTGENCTTAWTSICDT
                     AESIINPANSNPDSHGYVYWGTPTETRSGVAGVTAPAACTAPCNTNTDNGIYFRAPNG
                     DVVNIGRAKVTGMMIQMMKITSLGVGP"
BASE COUNT         2387 a         3397 c         2967 g         2173 t
ORIGIN      
        1 cgcaggcctg ccaccaccgc catcctgacc accgtcagcg aatagaggtg acagagcaaa
       61 acagccacag ccagagtcgc cgccaccgcc tgccaggccc gcccatgcca tcttcccagc
      121 caggaaacgg caggcagccc atcaagaaag acagcaaaag ccagcatgaa aaccggcatc
      181 gttgccacga agtagcgagg ctctataagt ccgcgatttc ccgagaccgt gtagatccag
      241 tgcccaacaa ccaaggcgac cccaaaatag aaaccccgca aggagcgacc tttcggcatg
      301 aacatgccaa tgagcgaaaa tacgaaaagg ggggcgggga agatatcgaa gaatccagcg
      361 cttattggcg tccatccata ggacatccct tgcttctggt agatttttgc cgcctctact
      421 cgccagccaa gaaacgagtc aatatctgag aaaatgaaaa tgctaatgaa aaatacaaat
      481 ccaaaagaaa gcaaggcagc caattcccgc cattttctct gataaaggaa aattgccgag
      541 aataccaaga caaagaatat cccttctggc cgcaccaggg gcatgaccgc ggtcgccaac
      601 aggaacagcc tcttatgacc ataaacatgg caaaaagcgg aggccgccac caataccaga
      661 agccatgatt ccccatagaa actcatgccc acgaaaaaat acaggggcat gactgccaac
      721 aggagggccg cccccagcgc cgaccaacgc gccaattgca gccctaggca ggtccggtag
      781 accagaaaaa cagaaaaggc cgacagcatt gccttgatgt aacgggcact gagcaagtca
      841 ccctggctca aggttgcaac caccccgtcc agaaaaaccc acgccggcag cgaaaagttg
      901 aaccagtaga tatccggttt tatccacgcc gatctcgcca ggaaccagcg gtaagcgtca
      961 tctccccaat aaacgttggt ttcagcaaca tgatcaacat aaatccatgc accatcacca
     1021 tgcaccaggc taatgatcac cccataaaaa gacgacagcg caagcactga gaaaatggca
     1081 gcccgccaac gccatatgta atcaaggaaa acctggaacc tttcattctt cataaatcac
     1141 taccacaaag actctcacct aaatcttcct gacaattttt tgtatggcct ttaaaaattg
     1201 gctggcaaag ttgtcgttgc tgccgagaaa actcttgata accgtataaa ttgacaccat
     1261 caggctatcg attcttatca ggaccttttt aaccccgtcg tcggggcgcg ccctatagtt
     1321 ggtggcccgc ttcaatggac tctcttcctc ggtgtaataa taaagtgcaa tcgagcgccg
     1381 aaagcagcca tcagggcagt tcatgggctc ggggtgacca tgaaacgagt tggcatcggt
     1441 attgaagatc acgcaacgat tgaataaagg tgccaccctc acttcgcagg tggtcattcc
     1501 cctgttccac agctcgagat ggccgcccca ttcgtcctgc cagtccttac cgaggtaaac
     1561 aataacgttc attcgccgcc gccagttacg atgatgcggg tgcaccgtaa aatcggcatg
     1621 gatattcaga aagccgcccc tggtgctctg atggatgccg cccccctcgc aggactcgtc
     1681 cgccttcagg ctttctatcc cggacagggc actcagaaaa cgcacgaaat cgggactatt
     1741 cagatgcctg atgactccct gcaaaccagg gggaagcgca tcgatcttgc tcattcccat
     1801 tttgttttca ttgaaatgga catagttgat ccaggcgtcg ctgttgtcca gggcctcaaa
     1861 gtcggcaagc gcaccttcaa gcgagtcttt gtccagaaag tcttccagca ccaaatgtgg
     1921 aaacggcgcg ttgccttgat accggctttt caagccagcc agctgggcac cccatctttc
     1981 ataatcaaaa atacgctttt gacccatgcc ttcattcata agcttgaact tttatcttga
     2041 gttattcgtt cgtccatgtg gaattcaatc tcacatcgct tacgcctgcc gttgattggc
     2101 atcccgggac aatatagagt gctgcaagcc caactgcaga ttaggcgttg taaagtcatg
     2161 cttgctacac ttcgccctta cgccattgtc atttcattgc ttatcatcgc cgtcctggct
     2221 atctatgggc agaccgccac cttcggctac gtcagctacg acgatcccgg ctacaccctc
     2281 caaaatcatg tcgtcaatgc cggccttact ctcgacggca tcaagtgggc tcttacctcc
     2341 accacctata tgtccaactg gcagcccctg gtctggatat cgtacatgct cgatgtcagt
     2401 ctgttcggcg cctaccgtcc cggtatccat cacctggtca acctgggact gcacctgacc
     2461 agttcgatca tcctgttcct ggtgctgacg agaatgaccc ggcaactctg gccaagtgct
     2521 ttcgtggccg ccctgttcgc cgtacatccg ctccatgttg agtcagtcgc ctggattgcc
     2581 gaacgcaagg acgtactcag cggcttgttc tggatgctta ccatggccgc ctatgtattt
     2641 tacagcgagc gccccagcat cgggcgttac ctgctggtct tcatcagcat ggcctgcggt
     2701 ctcgcgacca aaccgatgct ggtgacactg ccattcgttc tgcttctcat ggactactgg
     2761 cctctgcgac gaggtcttca attgccagcg tcagcagcag ccccctctcc cgatgcccct
     2821 cccctccggc tgatcctggc cgaaaagctc cccctcctcg ccctgtcgct gatctcgtcg
     2881 ctcatcacct atctggccca gcggcaggcc gaagccatga gcttcggcag cagcctgaca
     2941 gttgcacagc gcgtttcctc ggccatcatc tcctatgccg agtatatttt gaaaaccatt
     3001 tggccgacga atctggcggt catttacccc tacaacgaga gctgggcaac actgcaggtc
     3061 gtgatgactg cctcggcgtt gctcttcgtc accctgattg ttgttctgca ggcgcgccga
     3121 tttccctatc tgccggtcgg atggttctgg tttctgggca cgctgatccc ggtcattgga
     3181 cttgtacagg ttggaataca gggcatggcc gaccggttta cctatatacc gcatatcggg
     3241 ctcttcatcg ccgtggtatt cctgctggcg gacctgggac aacggcacgt gcgaatcagg
     3301 caagcctcca ccatcctcgg cggggtagcc gtcatcatcc tgacggctat tgccgcagtg
     3361 cagacaagct actggcgcag tagcgaggac ctctttcggc aagcggtaag agtccccggc
     3421 aactacgttg ccgagaccaa cctggccgcc atactgctcg acaagccctc gcggacgcag
     3481 gatgaactca tcgaggcctt tgctcgcgcc cggtacgctg taaccctgaa gaacaaccat
     3541 gccgacgcac actggattct cggggtggcc ctcgccaaca tgggcaagac agacgaggcc
     3601 atcctccagt acctcgaggc gatccgttac aacgctgatc acggaaccgc atgggcctat
     3661 ttagcagcaa tctatgctca aaataaactc atgccgaaag cagccgccgc ccttaatgag
     3721 gctgaaagga tcgctgcccg acaagcccaa ctgccccccc gcgccaatta tcggccagaa
     3781 ggcgacccgg atggccattt gcttctgcgt gagagatgag gactgccgag gaggccgcac
     3841 actgcggaat gcggaaggtg tcacagcgcg ctgcttacag gagctcaaat gtatcagggc
     3901 aagaagatca tcgtcgtcat gccggcctac aacgcggcca ggacactgcg tcgcacccat
     3961 gacgagataa tggcccaagg catcgttgac ctggtaattg ttgttgatga tgccagccac
     4021 gacgagacaa ttgccctggc gcagaccttg ccgaataccc tcgtccaccg ccacgcggtg
     4081 aatcgtggtt atggcgccaa ccagaagacc tgctaccgcc tggccctgga gaatggcgcc
     4141 gacatcgtca tcatgattca ccccgactat caatatacgc cactgctcat tccggccatg
     4201 gcatcgatgg tcgcgaacct gtacccctgt gtactgggta gtcgaatcct ggggggatac
     4261 gccctcacgg gcgggatgcc tctctggaag tacatcgcca accgcttcct gaccttcgtg
     4321 gagaacctgt tgacgggcgc caagctgtcg gaatatcaca cgggctaccg ggccttctcg
     4381 cgcgaactgc tgatgaggct gccgctcgag gagaactccg atggcttcat atttgacaat
     4441 gaggtgctgg cacagattct ctggacgggg accgtcatcg ccgaagtctc ctgccccacc
     4501 cgttatgacg cggaatcgag ttccatcaat ttcacgcaaa gcatcaagta cggcttcgga
     4561 tgcctggcag tgggcctgcg ctaccggctg gcccggtggg gcttgaagtc gaagcttttc
     4621 cctcgactgt aaaccagaag gcgggccgtc ggacctcagc gactcacggt atcttgggga
     4681 aaatggtcac ttgcgagcgc acgatgccgc tgggcatggc gagggagccc agggatttgc
     4741 ccccgaactc cagcgtattc accaccagcg gattgcccgc cgcctccgtg caggagacgc
     4801 cccaggcgca aggcaccgaa gaagacaggc ctacgtagag attattggac accaggagct
     4861 ggatcttgcc tgcccccagg tcgccctcac cctgcatctc gatcctgccc tgccccacac
     4921 cgacagagcc gcggacgtcc gcaaagcgca cgtcaatccc ccgcttgctg aagctgggct
     4981 cggcgaacga caagtagctc ccgtcatcat gggcgtgatt accggtgccg gacggcgagg
     5041 cgaaatcggc ctcgcttaaa ttcgccaggt tgatgaagcc ggaataggca agatagctgt
     5101 ccgtcacgcc ctgcccgggc ccgatcgtga acacgtagcg atctgcctcg acgttcacat
     5161 ccacataggc catctgctgc ggtatggtca tgtcagggat ggcgccgccc cccagcatgc
     5221 cacgcagctg gtagcgaaac agaggcgcat ccgtcttgat gcccgtactg gtcatgccca
     5281 tgcggccatt gcgaagggca atcaatacat cagcccccat caggccaatg ccaaaatccg
     5341 tatcgctatc accaatcatg aaattggtac ccgtattcca ccgggtacgg tcggtcgagt
     5401 ttgcatctgc ggcatgggtt tgcgacataa ccgccagatc caggcgtgcg gattgtccgt
     5461 cggcataggg agagacgaac aactctgcgt ccaggtcggc cagcgtataa atcagggccc
     5521 agttataagt ggtaacaacc ggcacacctg ccttgccggt gagggtacgg tccaccacct
     5581 tcacggttga ggcatacgcc gagagagcca tgtcgcggaa ggtgatggcc agcaccgaag
     5641 gatccgtgtc gcgtcgcacc ttgataggct gcactgcgtc cagcgacgcc accgggccta
     5701 cgccggctcc agaacaggcg ttgttggcgg tcgccgtgat ggcggcattg ccccagcaca
     5761 accctccctt cccctgggca gttgcgcccc cggccagggc ctcgatgctg aagaacggaa
     5821 tatcaaaggc ataagtgttg tttggcagct ttgtccagtt gccaaactcc aggtaacccg
     5881 gcttgtcgcc gccctcaccc acggcccaga caaaatcagg atcgtagttg aacttgatgc
     5941 tgctgttgat gccccggcca ctgccgtcta cacgaccacc atttttcagg aactccaggt
     6001 tcaaatcggt ccagtggcca cgccatcccc agaacagcat gggcttgtca gtcgccccga
     6061 tggcaaaggc ggaagagcca ctaccgtcat acaacatatc cagcgacagc gagaatccca
     6121 ctttggagcc tgtcttggat tgaatgacaa aactattgtt cttgaatcca aaccggccgt
     6181 ccgccagctc aaacaggaag gagaagtcat tcatcaccat ttccggcgat gtgccagcca
     6241 catcatcggc ccggcgccag aagatcttgc ccggatccgt cgcaccctgg taaccaggcg
     6301 cccaaccggc cttattgacc ttgccgaagt tcagggagag ggaagagccg tagcttgcgg
     6361 tgttggagtc gaacaggttt cgtccgacca ggctcaggtc gatgggagtt tcgatgagat
     6421 agtggcccag gctaccgcta ccaacaccgc ttgtcgttcc cgccagatta accgcgccga
     6481 tatctatttg agagcgcgcc agcccggcac tcagcgcgac gaattcatcg ctcgaggcag
     6541 tgggaacacc gaaatccagt gtggcagtga aaaaaggcga cgtgacgccc gtggcgcgtt
     6601 tgatattgat cgtggaaaga cgcaccccca gctcattggc ctcgtttggc gtccagggag
     6661 tggggttgtt ggtcagaagg gcggcactgc ttgccgtaat ccccgccgcc ggctcgttca
     6721 tcttgagcgt gatgccggcc tttgccacga cggaggacat ttcttcatcg ggcaatgcct
     6781 gcagggcact cgcgtcaaca gcaagcgccg tcagccccat ggagatggcg gccccgagca
     6841 acacacgatt attttctttc ttattcaggt tcattgcttg actcaatgtc agccgaaact
     6901 tgtgtctcac ccgcgcctca tcccgcctgc ggggaaaggg gccgcctcaa cagaacacgc
     6961 tggttcccca gcagttgtca tacacacgcc cccccgcatt ggccggcagg ccggtgatgt
     7021 tacccgccgt atcggagttg gagctcacga tattggacac cagcagggtg gtcgggcccg
     7081 cgatgttcaa ggcaaaggca ttgggcaaat acatgctcca gcccttctgc atggcgacgg
     7141 catagccggg gtactgcaca ctttctttct ggaaggacaa accgaacagg ctggaatcca
     7201 ccatggtgct gtggaagttt tccaggttga aaggcatctt gtagccgttc aacgcggcgt
     7261 cagaaacatt accggtatag gtgatgccca gaccggtggc cagctggttc ttgaggtccg
     7321 tcttcacctg gccgatcagg aagggcagga aggtattgag caagccggag ccaaggctcg
     7381 tggacgcctt gaccgagacg gagtcgccca gcgtaccgac cacgttcgcc aggttcaggc
     7441 cgctcaccag cgcctggctc aaacggctac ccgaggccac aacgttgcca ttggcattga
     7501 ccgtattgaa gcccaccagc agggttcggg tattggcgcc cgtgatcccg gcatcgacgt
     7561 actggttgtt aaaaccaaag ttgtagcccc ccgcacagcc ggtgttcgtg acgctggtgc
     7621 tggcacagct cagctgtgac tggttgaggt ggtcccagcg ggtattggcg actcggaaat
     7681 ccgagtcatt ggtcaaggcc acgcccgtac acatgttgtt ggtgcagcca ctcgggttgc
     7741 cgccgttgga ataccagccc acacggccat cattgccctg cccctgcatt tgcagagcgg
     7801 ccgttcccga catatagccg gagaaggagt tgaagttacc ggccgagaac gggccctggg
     7861 cattctgcgc gcccagacga atgcccacca cttctcgggt ggccgcattg tcgcccttga
     7921 ccgcaatctg gacaaaggga cgcaacaggg tgaacggctt caggaccttg ccggtctcag
     7981 tcgtgccact gccggccacg atgcaatccg ccgtcgtcgg actggcggtc gcgccaatgc
     8041 agcccatggc taagttcttg atccacaggt cgacaccgct ggtatcctca cccagtttca
     8101 gttcggcgat gttgacgtta aggtcgagct gggcatccag acccatcttg taaaaatcgt
     8161 aggtagtcgt ccccagcaca ttgcgttcat gcgacgacat gaacagggcc tgaccattga
     8221 cctgggccat ctcggaatca tccatggcgg tcagccccgc gggcacgggc gcaggacggg
     8281 ccccggccgc caccaacccc ttttcctcca gacgctgggt gcccgcctgg ttgatcgcgg
     8341 gcaggagcat cagccccacc accagcccca tcagcaagga catggccttg acagcgccac
     8401 gctgggaaat gcgccacttc ctcgcatcgg cccgcccccg cccccggaac accacgaggc
     8461 ccggctgcca ctttagcgag gcacgcatga atgccgtcat cagcaagccc acaatacgtt
     8521 caaccttcat gttcagaacc cgtagataag ggccgagccg ccaatggcca tcatggcgaa
     8581 gttgccgttg ttatccctga tctgcgtccc cagaaaggaa cccgcgttgg cgtcacgcag
     8641 atagcccggg acatagcccg ggctggcgga ggtacaattg gtcagggcgg tacaggtgtc
     8701 gtactcgatc gacatacggc cgatattgag gccgatctgc agactggtga agcccgtact
     8761 gacgcccgta cccggcgtgt atgtcgtggc gttgtagctg gcgacggggt aatacagctt
     8821 gatcgcaggc agggtatcga tggtggcata ggcacagctg gcgccaccgc ccggcaggag
     8881 gcagacgctg gcgccgagat tgtcgtagcc catgaatctg tcattatcaa agtaggtgtt
     8941 attggcgccg acgccccccg cgccccccat ggtggcctgc acatcgatat tgagcgccgg
     9001 gatcttcacg ctcagccagc cgttcttggc caccgtccac gcggcgttgc cgttgcccgt
     9061 gcgcttggcg acttgccagg caaaacggca gcggtcaccg ccggtcgccg tcacggtgcc
     9121 gctcccggcg cagctcccca gggtcgccag gggcgcgccg gacgtgtccg tattcacgcc
     9181 atattcccaa ccgatggcaa tgccatcccc cccggacaca ccggcgagct cgccatcatc
     9241 cagcggccgc aagtccgccg ccatcacggg aaacgccacc agagccatca cgaagcctga
     9301 caggagtttc atggctcaag gccccacgcc cagtgaagtg attttcatca tttgtatcat
     9361 catgcctgtg accttggcgc gaccgatatt gacgacatcg ccattgggag cacggaaata
     9421 gatgccgttg tcggtattgg tgttgcaggg cgcggtacag gccgcgggag ccgtcacccc
     9481 cgccacgccg cttcgcgttt ctgtcggcgt gccccaatag acataaccat gggagtccgg
     9541 attggaattg gcgggattga tgatgctttc agccgtatcg catatcgagg tccaggcggt
     9601 cgtgcaattt tcacccgtct tcaccttgac cgaccgatcg tagatcccgg tacaggccac
     9661 cacattgccg agctggttga cgcagccaat ggcggcattg ctgccattca ggccaacccc
     9721 gcccgtggcc tccggaatcc cgctcgttac gaaactagcg cagttgttct ggccgcaata
     9781 gaaggtgttc gacacattgg tgttgctgga aatgggcgtc agctcaaccg tgaaattacc
     9841 ggaggtgccg ttgttgcgga aaaccacgga ctggtaattg ttctggccca gggggagaaa
     9901 ggcatcgacg ttcttgaaaa ccatgccttc gacgtcattg aagtccggca cgatgccgtg
     9961 cgcaaccgat tgcgcgtagg tgcccgtttg cgccaccgag aaacggaaat taccggaaag
    10021 acggctctgg taaaccacgc tcagggattg ctcgctgacg gtcgtggtcg gggtctgcac
    10081 gatctgcagg cgcgtgggct tgttgtccag cgtgaagtta ttgccgagaa tgatggtctg
    10141 gctcttgagg atgcccgcgc aattcgcgcc tgccgtcacc gaagcgccat tacaggtgcc
    10201 ggagaacgtg tcgcccgccc cgcccagggc cagcgccccg ttattgacca gcagttcgcc
    10261 ccagaacgcc cagcgccatt tgtcgccctt gctcgggccc acgaattcgt aataggtcgg
    10321 cgaggtggcc aggctcacct ggctgcactt gccactgact tccgaaccca ggcaagtgcc
    10381 ttcgtagctg tagcctgcat actggaagac gcggatcatg taggggttgt acaccgaggc
    10441 ccagttcttg atgccctcga cctggctgct gccgttgata cgaccgatcg ggcagcccag
    10501 tccatcagcc cccggggtac agcccccgcc tgccgtcccc gtgccatacc agtccatgcc
    10561 atagttaaca ttaccgccag acatggacag gccgtagtaa tacagatcgc cacgacgcca
    10621 gttgctcggc gcgtcatacg gatcacatga ggaggcagga agcgcgggcg aaccggaagc
    10681 cgaatttctg cacccccctt ccccgccatt gagcgtcgcg tcgaaaacaa tgccggtgtt
    10741 atccacgaca ttcgtcccca gcatattgat gtagctggtc ggtgcgaatc gcgagctgaa
    10801 gttgtcgaag gccaacgcga cgcccgtgcc attgacctct gccagggcgc cgtcatcaag
    10861 cgattccagt gcatgcgcct gcgtgcccat agccaaagag accacgagca gacagcttcc
    10921 gacg
//
LOCUS       DASUEG010000200         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2080917, whole genome shotgun sequence.
ACCESSION   DASUEG010000200 DASUEG010000000
VERSION     DASUEG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080917"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFX11_03795"
     CDS             <1..1403
                     /locus_tag="VFX11_03795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX4937771.1"
                     /translation="YAASQGLDIAQLRQQCPRKSEKPFDAAWKYMRVTVLEDGQCISY
                     YKGAPEMLLGLCTLTAEQRQICQATINRHAARGYRLLALAREQGANETGLQWLGTALL
                     WDPPRPEVADAIARCRSAGVRVLMITGDHPATARAIADHVGIQAGRTVTGDELSGLST
                     TQLQSLLMDATVFARVKPEHKLAIVQALQAGGDVVAVTGDGVNDAPALKAADVGVAMG
                     LRGSDVSREVADLVLLDDNFSTIVAAIEEGRSIYENIQKFIRTLFSTNVTEVMLIAIG
                     AMLAFYFTDAGVALLLPLTAAQVLWVNLLTDSLPALAITTDTNPGLLRRRPRKAVSPL
                     MDPATLIYVFLVGGVGGIVALLLFWWLPASGTSPAEAQTVVFCYLVFVQMTFVPPARR
                     VNVSAVWNRWVFLALLVGVVAQLAALLNDELGRFVSVEKLSASSTGVLAVSVTLSWLV
                     AEATALALRRRRSKGW"
     gene            complement(1474..1698)
                     /locus_tag="VFX11_03800"
     CDS             complement(1474..1698)
                     /locus_tag="VFX11_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937772.1"
                     /translation="MKMVNSNMLRQLAIDSLSFMVVLALSLGGLWGLFLVDASLFTMV
                     VFGLLMIPALFSSTYHLGKDINEATHKLIA"
     gene            2204..3415
                     /locus_tag="VFX11_03805"
     CDS             2204..3415
                     /locus_tag="VFX11_03805"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4937773.1"
                     /translation="MTTEAYVFDAVRTPRGKGKKDGALHEVKPVDLVVNLMHALQARN
                     PGLNTAQIDDIVLGIVSPVGEQGAVLPKTAAMKAKWATEVAGMQINRFCASGLETVNL
                     AAQKVASGWEDLVVCGGVESMSRVPMGSDGGAWAMDPQTNLMTDFIPQGIGADLIATI
                     EGFSREDVDTFAYNSQMKAARAKADGSFSKSIVPVRDMNGIVILAEDEYNRADTSLEG
                     LGKLQPSFLMMGQMGFDAVALQKYHMVEAINHVHTPGNSSGIVDGASLVLIGNKETGA
                     KMGLKPRGRIVATAITSTDPTIMLTGPAPAANKALKKAGLTYKDIDLFEVNEAFASVV
                     MRFMKDTGVPAEKINVNGGSIALGHPLGATGGMILGVVLDELERRGLKRGLCTLCVGA
                     GMGIATIIERV"
     gene            3438..>3961
                     /locus_tag="VFX11_03810"
     CDS             3438..>3961
                     /locus_tag="VFX11_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4937774.1"
                     /translation="MTDHIRYEKDSDNIVVLTMDMVGQSANTMNLNYQQAMDACLDRL
                     EKEEKLAGVVITSAKKTFFAGGDLNDLIKASPEVAEAFMNGTLLVKKQLRRIEKLKAP
                     VVAAINGAALGGGYEICMACNHRIAINDAKTKIGLPEVTLGLLPGAGGVVRLTRMLGL
                     EAAAPFLMEGKQVSP"
BASE COUNT          823 a         1086 c         1219 g          833 t
ORIGIN      
        1 actatgccgc ttcccaggga ctggatatcg cccagctgag gcagcagtgt ccccggaaaa
       61 gtgaaaagcc gttcgatgcc gcctggaaat acatgcgggt cacagtgctg gaagacgggc
      121 agtgcatcag ctactacaag ggagcgccgg aaatgctgct gggtctgtgc acgctgacgg
      181 cggagcagcg gcagatctgt caagccacca tcaaccgaca tgccgcgcgg ggttaccgcc
      241 tgctggccct ggcgcgggag cagggtgcca atgaaactgg cctgcaatgg ctgggtactg
      301 cgctgctgtg ggatccgccc cggcctgaag tggccgatgc catagcccgt tgccgctctg
      361 cgggtgtacg ggtgttgatg atcaccggcg accacccggc taccgcgcgg gccattgccg
      421 atcatgtcgg catccaggcg ggcaggacgg tcacaggaga tgaactgagc gggctgtcca
      481 ccacccagct gcagtcattg ctgatggatg ccactgtctt tgcccgggta aaacccgagc
      541 acaaactggc cattgtgcag gcattgcagg caggcggcga cgtggtggca gtgaccggtg
      601 atggagtaaa tgacgctcca gccctgaagg cggcagatgt aggcgtggcc atgggactgc
      661 gcggcagcga tgtcagccgg gaagtggcgg atctggtgct gctggacgac aacttttcca
      721 ccattgtggc cgccattgag gaaggtcgca gtatctacga aaacatccag aagtttatcc
      781 gcaccctgtt ttccaccaac gtcaccgagg tgatgctgat agcgataggt gcgatgctgg
      841 cgttctattt tacggatgct ggtgtagcac ttcttttgcc gctgacagcg gcgcaggtgc
      901 tgtgggtgaa tctgctgaca gattccctgc ctgcgctggc catcaccacc gacactaatc
      961 caggcctgct gcgcaggcgg ccgcgcaagg ccgtttcacc actgatggac ccggctaccc
     1021 tgatctatgt atttctggtg gggggcgtag gaggcattgt cgcgctgctg ttgttttggt
     1081 ggttgccagc gtctggcacg tcaccggcag aagcccagac cgttgttttc tgttacctgg
     1141 tgtttgtgca gatgacgttt gttccacccg cacgcagggt caatgtgagt gccgtctgga
     1201 atcgatgggt ttttctggcg ttactggtag gcgttgtcgc gcagctggcg gccctgttga
     1261 atgacgaact gggtcgattt gtttcggtgg aaaagctgtc tgcatccagc accggcgtac
     1321 tggcggtatc cgtgacgctc agctggctgg ttgcagaggc tacagcactc gcattgcgac
     1381 gtcgacggag caaaggctgg taaaacagcg agaagggaat ttgttgccac agcgggcgtc
     1441 acctctacag ggcgcccggc tgcggcgatc gtgtcacgca atcaacttat gcgtggcttc
     1501 gttgatgtcc ttgcccaggt ggtaagtgct gctgaacagg gccgggatca tgagcaggcc
     1561 gaacactacc atggtgaaca gggaggcatc taccaggaac aagccccaca gtccgcccag
     1621 ggaaagggcc agcacgacca tgaatgacag gctgtcgata gccagttgac gcagcatgtt
     1681 tgaatttacc attttcatac tcgaactcct cgtgtgtatc tgacacagcg ttaagccaga
     1741 tctgcttgcc tcacagtgcc agttcgttgg agttctctct ggcggtctgt taacccttca
     1801 cagggtcttg gcgagcagtg tgccttcttg ggaagtcacg tgatttaatc tagacctgaa
     1861 aattagagtt ttcctactaa tccaccctaa tcaactttct ggtcttaccg tattccgtgc
     1921 ttccatgttc actactatag cgctggttta accaatttca agcttttgcg tgtgtgaata
     1981 tgtaacactt gggcagaatg gtaacagtgt cacatggcaa aatggttaca tacctcataa
     2041 gttccgtgtc tctatcccgt ttggttacct aatgagctga tgctttttgt cacctccgct
     2101 gatctgtacg gtcattgtct ggatttggca aaaagcgcaa ggtatgcagc ctgagtgtcc
     2161 cgtgggtcac ggactcaaaa ccgaaactat gaggaatggt tgaatgacta cagaagccta
     2221 cgtcttcgat gccgtgcgca ctccgcgtgg caagggcaaa aaggatggcg cactgcatga
     2281 agtgaagccg gttgatctgg tggtaaacct gatgcatgcc ttgcaggcgc gcaatccggg
     2341 cctgaatacc gcgcagattg acgacatcgt gttgggcatc gtcagcccgg tgggcgagca
     2401 gggtgcagta ctgcccaaga ctgccgcaat gaaagccaag tgggccactg aagtggccgg
     2461 catgcagatc aaccgtttct gcgcctcggg cctggagact gtcaacctgg cggcccagaa
     2521 ggtggcctct ggctgggaag atctggtggt gtgtggtggc gtggaatcca tgtcccgcgt
     2581 gccgatgggc tccgacggcg gtgcctgggc tatggatccg cagaccaacc tgatgacgga
     2641 tttcatcccc cagggtattg gtgcggacct gatcgcgacc attgaaggct tctcccgtga
     2701 agacgtggat acctttgcct acaactccca gatgaaagcc gcccgcgcca aggccgacgg
     2761 cagcttcagc aagtcgatcg tgccggtccg cgacatgaac ggcatcgtga tcctggcaga
     2821 agacgaatac aaccgcgccg atacttcgct ggaaggtctg ggcaagctgc agccctcgtt
     2881 cctgatgatg gggcagatgg gcttcgatgc cgttgcgctg cagaaatacc acatggtgga
     2941 agccatcaac cacgtgcaca cgcccggtaa ctcctcgggc atcgttgatg gtgcatcact
     3001 ggtgctgatc ggcaacaagg aaaccggcgc gaagatgggc ctgaagcccc gcggtcgcat
     3061 cgtggcaacg gccattacca gcaccgaccc caccatcatg ctgaccggcc cggctcccgc
     3121 tgccaacaag gcgctgaaga aggccggcct gacctacaag gacatcgacc tgttcgaagt
     3181 gaacgaagcg ttcgcatccg tggtcatgcg cttcatgaag gacaccggtg ttcctgccga
     3241 gaagatcaac gtgaatggtg gctcgattgc gctgggtcac ccgctgggcg ctaccggcgg
     3301 catgattctg ggtgtcgtgc tcgacgaact tgagcgtcgc ggattgaagc gcggcctctg
     3361 caccctctgc gtcggcgcag gcatgggtat cgccaccatc atcgagcgcg tgtgagaaca
     3421 ggattcggag actgacaatg actgaccata ttcgttacga aaaagacagc gacaatatcg
     3481 tcgtgctgac catggacatg gtgggccagt ccgccaacac catgaacctg aactaccagc
     3541 aggcgatgga tgcgtgcctg gaccgtctgg aaaaagaaga aaagctggcc ggcgtggtca
     3601 tcacgtccgc caagaaaacc ttcttcgccg gcggtgacct gaacgacctg atcaaggcca
     3661 gcccggaagt ggcggaagcg ttcatgaatg gcactctgct ggtgaagaag cagttgcgcc
     3721 gcatcgagaa gctgaaagcg ccggtggtgg ccgccatcaa cggtgctgcc ctgggtggtg
     3781 gttacgagat ctgcatggcg tgcaatcacc gcatcgccat caatgatgcc aagaccaaga
     3841 tcggtctgcc ggaagtgacc ctgggcctgc tgccgggcgc cggtggtgtg gtgcgtctga
     3901 cccgcatgct gggtctggaa gcagccgctc ccttcctgat ggaaggcaag caggtgagtc
     3961 c
//
LOCUS       DASUEG010000201         9626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_991241, whole genome shotgun sequence.
ACCESSION   DASUEG010000201 DASUEG010000000
VERSION     DASUEG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991241"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..543
                     /gene="rpsP"
                     /locus_tag="VFX11_03815"
     CDS             292..543
                     /gene="rpsP"
                     /locus_tag="VFX11_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4937775.1"
                     /translation="MVTIRLARGGAKKRPFYHLVVTNSRSARDGRFIERVGFFNPIAT
                     GGEDRLRINFERVEYWIGTGAQPSERVAALIRDAKKAAA"
     gene            552..1070
                     /gene="rimM"
                     /locus_tag="VFX11_03820"
     CDS             552..1070
                     /gene="rimM"
                     /locus_tag="VFX11_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930638.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4937776.1"
                     /translation="MNDWVVVGKFMSPFGIKGMLKLFSHTQPIENIAEYNPLWINRGG
                     SWQPINLEHVQRHGKGLVAKIKGCDDRDQTPAYIGCEVAIKREQLPALEQGDYYWSQL
                     EGLAVITTTGDRLGKVDHLLETGSNDVLVVQPTADSIDRQERLIPYLWQDVVTQVDLQ
                     AGTITVDWDKDF"
     gene            1116..1871
                     /gene="trmD"
                     /locus_tag="VFX11_03825"
     CDS             1116..1871
                     /gene="trmD"
                     /locus_tag="VFX11_03825"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008485149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4937777.1"
                     /translation="MKCSIVTLFPDMFKAITEHGITSRAVKNGIIELETVNPRDFTHD
                     VHRTVDDRPYGGGPGMLMLVEPLLAALEQARQNLAAKGVTQPKVVYVSPQGKRFDQHA
                     AREFSRQDGIIFLCGRYEGIDERVIELAVDEEWSIGDYVLSGGELPVMAMIDAAARLL
                     PGVLGHKDSAAEDSFFEGLLDCPHYTRPEDYQGRVVPPVLLSGNHAAIRRWRLKQSLG
                     RTWQRRPDLLQGRVLSKEEQTLLREYQDEQGDA"
     gene            1961..2317
                     /gene="rplS"
                     /locus_tag="VFX11_03830"
     CDS             1961..2317
                     /gene="rplS"
                     /locus_tag="VFX11_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4937778.1"
                     /translation="MSGKNKIIQMLEKEQMKTDVPTFGIGDTVVVNVKVQEGDRSRLQ
                     AYEGVVIAKRNRGLNSAFTVRKISNGVGVERVFQTHSPLVDSISLKRRGAVRRAKLYY
                     LRDRAGKSARIREKLN"
     gene            complement(2424..2849)
                     /locus_tag="VFX11_03835"
     CDS             complement(2424..2849)
                     /locus_tag="VFX11_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937779.1"
                     /translation="MLRRGALITTLLLLLSPPATADIAIITHPDNPVANLNQKEVQKI
                     FLGRLRMYPESELETQAVDLPAEDPLFLRFYDTIARMNAAKIKRYRAYYLFSGKGRLP
                     LVEPDEKHVMARVASDPSAIGYVDASLVTDQVRVVFVLK"
     gene            complement(2851..4137)
                     /locus_tag="VFX11_03840"
     CDS             complement(2851..4137)
                     /locus_tag="VFX11_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937780.1"
                     /translation="MLREPWSNLLHLMKNRTLINILLAGLLLACCGPNVRATENWEWS
                     GFASFGGGQINRDQLKFIDYDDDWSFNSDSVAGLHTQGDITEHWSLTAQVIAQGHHWD
                     DTGDFHPELDWLFLSHALSPGLRLRLGRMRSPHYLFSETLDVGYSYPWVRPPVEVYTF
                     FMSPFSNFDGMDLTWNTELGDADLDIQALAGTMDGEFLGLDIEVEPVAGANVTLRQGE
                     WLLRYGVLLHRTDLTNAGSQAAADAFNQLAAIDPQFEEVANSFEADNAWYQYHALGGQ
                     WDHGNWTLLGEAFEVIGPDEGFSTNASGWYLSWQYHFARLAPYITTGHYKNTLNEDQI
                     QLLEDSFITYPPGSLGPAVDALRYGTIDAFRNFYSEQYSWTLGVRYELLANTSLKCEV
                     QYFDFIGGSTGHMIPEPGAPRPDDALLTSFIVDVVF"
     gene            4251..6068
                     /locus_tag="VFX11_03845"
     CDS             4251..6068
                     /locus_tag="VFX11_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937781.1"
                     /translation="MLRLDQNAKIRLNILLIALLGGLGFLCYLLVNVYLAQKNGERLD
                     NLLTRQYPVIEQIRLLKQDAQAIRDSLTAAVGLEDAFMVEDSQETATQFRERLRQLQA
                     MEPALTPVTQGIGAAFDRYYEKSQALALELIENQDNLSLFKRRVDDSHRDFAQLAQRV
                     DDLQRQRQQEYEALLLLTNAAVKDANLLGAILGALAIAGLGLLAWTISVRVLVEINRS
                     NRLKNEFLATISHELRTPMNGIIGALNLLRSGSLTPEQSSWVGVASRAASAMMMSIDD
                     ILQFSEILSGQSARHDAEFDLRAALEKLLATFRPEFAARQLAFSWHLDDALPPAVSGD
                     EQKILHVVRQLVSNALKFTESGRVAVQVSLAQPAEAPPPSDAVHMVCVRVQDSGPGIA
                     TERMRELFQPFQQLDGAFNRRHQGMGIGLAICRAIATLLGGTVELRTPEQGGVEAEFR
                     FPVGFKALSAVTSPEPVAPVIRSDALILVAEDNPVNQMVLKGYLAKLGFRVVTAGNGQ
                     EAVAAAQNNPVQLVLMDCQMPVMDGFEATRRIRQLPPPAGQVPIIAVTANALETDRQN
                     CLAAGMDGYLKKPVDIEALRASLQPYLAVPAYPAGVARD"
     gene            complement(6004..6477)
                     /locus_tag="VFX11_03850"
     CDS             complement(6004..6477)
                     /locus_tag="VFX11_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937782.1"
                     /translation="MLLLALLALGSGSARADIAIIVHPQNPLTELSEDDLKKIFLGRL
                     PLFPQTGQEIHPLDLPEEHTTYADFYRRLLQLDGTKLKRYRAYYLFSGRGRLPAGANS
                     VVEMIRKVSEDTAAIGYVDAAHVSDKTRTLLILRTSPERPRQDTQARQGKAAATP"
     gene            complement(6513..7763)
                     /locus_tag="VFX11_03855"
     CDS             complement(6513..7763)
                     /locus_tag="VFX11_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4937783.1"
                     /translation="MSKANHLFTKGKHLLLGLLASSPALAGDDWSFSGFASLGAGRVE
                     DSAYTYMDYNGEEWSYESDSALGLHARGNLAERLSLTVQVVSRGHNWDDTAEFEPELD
                     WLFLSYQLDSEWRVRLGRMRTPHYLYSETLDVGYSYVWVRPPVDVYSPILSPFSNFDG
                     ADVAWITDWRDFTIDLQLLAGRMERSRDSLNIEVEPMAGGNLTVQKEEVTLRYGLIFD
                     RTDIEVDAYEEAESAYRAAASIDPLFDDIANSLSADDAWYRYQTLGARWEHGDLALIG
                     EVFDIQNLDEGYTNNANGWYTSLQYRLGKFTPYCVAGQFTDRFNDEMKDDIQASYAVV
                     PAGYYAVLDDLRTTSLAVVNGLNYEQNTWTLGVRYELLSNLSLKGEWQYFEFAYGSSG
                     QMLQQTGEATQYTSLTTFVLDLVF"
     gene            7947..>9626
                     /locus_tag="VFX11_03860"
     CDS             7947..>9626
                     /locus_tag="VFX11_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937784.1"
                     /translation="MERNARIRLQIMLIAVIGGLAFLSYLLVNVVLAAVNADRLDRLQ
                     HHHYPIIEEIRLLQQDLNSMREGFATAVGLEDPVLLEDTRQVVQHARARLARLQTRDP
                     DLTDEVEGISAAFEAYVQPALLLTEALVADPRQLPGYKGAMEASLRDYQLLSDWIGGL
                     HAARQVAYRNLLRETNLAVNRANLWGAVLGALVIVALGVLAWAISARVLREINRSDRL
                     KDEFLSAISHELRTPMNGIIGALSLLRATTLDREQVSWLDVANRSASSMMSSIDDLLQ
                     FSELMAGQEALHVGEFRLRGGLTRLLAALRPEFEDKNLALEFYGGVVLDRVLVSHEGK
                     ILYVIRHLLSNALKFSDHGPVCLSIGQANRSAEVGEGSIRVLVQDSGPGIPEPLLAEL
                     GRPFRQLDGSFSRRHGGMGIGLATCHAIAHLLGASLTLRNRESGGLEVEFIVPVKFGD
                     TAPESGPDSTTLTGGSYRRSGLVLVAEDNLVNLMVLKGYLKHLGCNVVSARNGYQALD
                     LMQSKLVDLVLMDCQMPLMDGLEATRQIRKLPEPLRDTPIVALTANATDADR"
BASE COUNT         1908 a         2640 c         3099 g         1979 t
ORIGIN      
        1 tccaagggcg gcatgaagaa catgatgagg ggcatgaagg gaatgatggg cggcgggggt
       61 atgccaccct tcaagatgtg acggaaagcg ggactttcca tcggggtgga tttcccgtag
      121 aattcagcgc cttctgtgcc ctgcacaccc tcaagagccg gcaggaacag taagcaaagg
      181 ctgcacaaca tcaaatattt cagtgggtta ctgaggcccg aaacgggttt tggtgatctg
      241 ctttactttt aaatcgctga acagatagct tgaataaagg aaattgaaac catggtaact
      301 attcgtcttg ctcgtggcgg cgccaaaaag cgtcctttct accacctggt agtgaccaac
      361 agccgcagcg cccgcgacgg ccgtttcatc gagcgcgttg gcttcttcaa ccccatcgcc
      421 accggtggtg aagaccgtct gcgcatcaac ttcgagcgcg ttgaatactg gatcggcact
      481 ggcgcccagc cttcagagcg cgttgccgcc ctgatccggg acgccaagaa agcagcagcc
      541 tgaacaccgg catgaatgac tgggttgtcg tggggaaatt catgtccccg ttcgggataa
      601 aaggcatgct gaagcttttt tcccataccc agcccattga aaacatagcg gaatacaacc
      661 ccctctggat caatcgcggg ggtagctggc aacccatcaa ccttgaacat gttcagcgac
      721 atggcaaggg gctggtggcc aagatcaagg gttgtgacga ccgtgatcag acaccggcct
      781 acatcgggtg tgaagtagca atcaagcgcg agcagttgcc agcgctggaa cagggtgatt
      841 attactggtc ccaattggag gggctggcag tgattaccac cacgggtgat cgcctgggca
      901 aggtggatca cctgctggaa accggttcga acgacgtgct ggtggtgcag cccaccgccg
      961 acagtatcga ccgccaggaa cgactgattc cctacctgtg gcaggacgtg gtcacccagg
     1021 tcgatctgca ggcagggacc atcaccgtgg actgggacaa ggatttttga ccgtctgcgg
     1081 gcgggtcagt tggatctaga gtcagggtgg cccacatgaa atgttccatc gtcaccctgt
     1141 tcccggacat gttcaaagcc attaccgagc atggcatcac cagccgggcc gtgaagaacg
     1201 gcatcatcga gctggagacg gtgaaccccc gggatttcac ccacgacgtg catcgcacgg
     1261 tggatgatcg cccctacggt ggcggccctg gcatgctgat gctggtggag cccctgctgg
     1321 cggcgctgga acaggccaga cagaatctgg cggcaaaggg cgtgacgcag ccgaaggtgg
     1381 tgtatgtgtc gccccagggc aagcgcttcg accagcatgc ggcccgggaa ttcagccggc
     1441 aggatggcat catcttcctg tgcgggcgtt acgaaggtat tgatgagcgc gtgatcgagc
     1501 tggcggtgga tgaggaatgg tccatcggcg attacgttct cagcggtggt gaactgccgg
     1561 tgatggcgat gatcgatgcc gccgcacggt tgttgccggg tgtgctgggc cacaaggatt
     1621 ccgccgccga ggattcgttt ttcgaggggc tgctggattg cccccactat acccggcccg
     1681 aggattatca gggcagggtg gtgccaccgg ttttattgag cggcaaccat gccgcgatcc
     1741 ggcgctggcg actgaagcag tcgctgggcc gaacctggca gcgccggccc gatctgttgc
     1801 agggccgggt tctcagcaag gaagagcaga ccttgctgcg ggaataccag gatgaacagg
     1861 gtgacgcctg aaggcgctgc caccgaaccg attttttaac gacgtgaatg tagcgttatt
     1921 caaccctacc tctgacgtca taacgtagag gaaatctacc atgagcggta agaacaaaat
     1981 catccagatg ctggaaaagg aacagatgaa aaccgacgtg ccgaccttcg gcatcggtga
     2041 cactgttgtg gtgaacgtga aggtgcagga aggcgatcgc agccgtctgc aggcttacga
     2101 aggcgttgtg attgccaagc gtaaccgcgg cctgaactct gctttcaccg tacgcaagat
     2161 ctccaacggc gtgggtgttg agcgtgtatt ccagacgcac agcccgctgg ttgacagcat
     2221 ctcgctgaag cgtcgtggtg ccgtgcgtcg cgccaagctg tactacctgc gcgaccgggc
     2281 cggcaaatct gcccgtatcc gcgagaagct caactgatct ccgatccgtt ccggcttcca
     2341 ataaaaaagc gcctttaccg gcgctttttt attgccttca tatttcctgt tgtacctggc
     2401 tggtggtgtt gccagtccag ccgctatttc agaacaaaca ccactctcac ctggtctgta
     2461 accagactgg catccacgta gcctatggca gaggggtcgc ttgccacgcg cgccatgacg
     2521 tgtttttcgt caggttcaac aagcggtagc cggcccttgc cgctgaacag gtaataggcg
     2581 cgatagcgct tgatcttggc ggcattcatc cgggcgatgg tgtcgtagaa gcgcaggaaa
     2641 agtggatctt ccgcgggcag atccactgcc tgggtttcca gttctgattc cgggtacatg
     2701 cgcagacggc ccagaaaaat cttctgtact tccttctggt tgaggttggc taccggattg
     2761 tccgggtgag tgatgatggc aatgtccgcg gttgcaggtg ggcttagcag aagcagcagt
     2821 gtagtgatca gggctccgcg tcgcagcatg tcagaacacc acgtcgacga tgaagctggt
     2881 caggagtgcg tcgtccggcc ggggggctcc aggctcggga atcatgtggc ctgtgctgcc
     2941 tccgatgaag tcgaagtatt gaacctcgca tttcagactg gtgttggcca gcagttcgta
     3001 gcgtacaccc agggtccagc tgtactgctc ggagtagaaa ttccggaaag catcgatggt
     3061 gccatagcgc agtgcgtcaa ccgcagggcc cagcgagccg ggtgggtagg tgatgaaact
     3121 gtcttccagc agctgtattt gatcttcatt cagtgtgttc ttgtaatggc cggtggtgat
     3181 gtagggggcc agtcgcgcaa agtgatattg ccaggacagg taccagccgc tggcgttggt
     3241 ggagaatcct tcgtccggac ctattacctc gaaggcttcc cccagcaggg tccagttgcc
     3301 gtgatcccac tgtccgccca gggcgtgata ctgataccac gcgttgtcgg cttcaaagga
     3361 gtttgccact tcctcaaact gcggatcgat ggcggccagc tggttgaaag catctgcggc
     3421 ggcctggctg ccggcattgg tcaggtcggt gcgatgaagc aggacgccgt agcgcagcag
     3481 ccattcgccc tggcgcaggg tgacgttggc gcctgccacc ggttcgactt cgatgtccag
     3541 gccaagaaac tcgccgtcca tggtgccggc cagcgcctgg atatccaggt cggcgtcacc
     3601 cagttcggta ttccaggtca ggtccattcc atcgaagttg ctgaacggcg acatgaagaa
     3661 ggtgtaaacc tccaccgggg ggcgcaccca ggggtaggag tagcccacat ccagggtctc
     3721 ggagaacagg tagtggggcg agcgcatgcg gcccaggcgc agacgcaggc ctggtgacag
     3781 ggcgtggctg aggaacagcc agtccagctc gggatggaag tcgccagtgt catcccagtg
     3841 atggccctgg gcgatgacct gtgcggtcag tgaccagtgc tcggtgatat cgccctgggt
     3901 gtgcaggccg gccacggaat cggagttgaa gctccagtcg tcgtcgtagt cgataaattt
     3961 gagctggtca cggttgatct ggccaccgcc aaaggaggca aagccagacc actcccagtt
     4021 ctcggtggcc cggacattgg gtccgcaaca agccagcagc agaccagcca gtaagatatt
     4081 aatcagcgtg cggtttttca tcaggtgcag caggtttgac cagggttccc taagcatagc
     4141 caacatctgc cggaccaacg tgcatcccgc ggtggcctgg cggtgggctg ctagactttt
     4201 gcaaggctgt gccgcgtggc gcagccattg tcgccggtcg ggggaatgct gtgctgcgcc
     4261 tggatcagaa cgccaaaatc cgcctcaaca ttctgctgat tgccctgctg ggcgggctgg
     4321 ggttcctctg ttatctgctg gtgaatgtct atctggcgca aaagaacggc gagcggctgg
     4381 acaatctgct gacccgtcag tacccggtca tcgaacagat ccgccttttg aagcaggacg
     4441 cacaggcgat ccgcgatagc ctgacggcgg ccgtgggcct ggaagatgcg ttcatggtgg
     4501 aagattccca ggagaccgcg acccagttcc gtgagcggct tcgacagctg caggcgatgg
     4561 aaccggctct gacgccggtg acgcagggca tcggcgcggc tttcgatcgc tattacgaga
     4621 agagtcaggc tctggcgctg gaactgatcg aaaaccagga caacctgagt ctcttcaagc
     4681 gccgggtgga tgattcgcac agggactttg cccagctggc gcagcgggtg gatgatttgc
     4741 agcgccagcg tcagcaggag tatgaggcct tgctgctgct gaccaatgcg gcagtgaagg
     4801 acgccaatct gctgggggcc attctgggtg ccctggcgat tgccgggttg ggcctgctgg
     4861 cctggaccat cagcgtgcgg gtactggtgg aaatcaatcg cagcaaccgg ttgaagaacg
     4921 aattcctggc caccatcagc cacgaactgc gcaccccgat gaacggcatt atcggcgccc
     4981 tgaatctgtt gcgttccggt tcgctgacgc cggaacagag cagctgggtg ggcgtcgctt
     5041 cgcgcgcggc atcggcgatg atgatgtcca tcgacgatat cctgcagttc agcgagattc
     5101 tctccgggca gagtgcgcgt cacgatgcgg aattcgatct gcgggcggcg ctggaaaaac
     5161 tgctggcgac gttcaggccg gaatttgccg cgcgccagct tgccttcagc tggcatctgg
     5221 atgatgcact gccacctgcc gtgagcggag atgaacagaa aattctgcat gtcgtgcgtc
     5281 agctggtgag caacgcactg aagttcacgg agtccggccg ggtggccgtt caggtgtcgc
     5341 tggcccagcc ggcagaagcc cctccaccgt ccgacgcagt gcacatggtg tgtgtgcggg
     5401 tgcaggattc cggtcctggc attgcgacgg aacgcatgcg tgaactgttc cagccgttcc
     5461 agcagctgga tggcgcgttc aaccggcgcc atcagggtat ggggatcggt ttggcgatct
     5521 gtcgtgccat tgccaccctg ttgggcggta cggtggaatt gcgcacgccg gagcaagggg
     5581 gggttgaggc agagttccgt tttcccgtgg gcttcaaggc cctgtcggca gtaacgagtc
     5641 cggagccggt cgcaccggtc atcagatccg acgcgctgat cctggtggcc gaagacaatc
     5701 cggtgaacca gatggtgctc aagggttatc tggccaagct gggatttcgg gtggtgaccg
     5761 cgggcaatgg ccaggaggca gtggcggcag cccagaataa tccggtgcaa ctggtgctga
     5821 tggactgtca gatgccggtc atggatggct tcgaggcaac gcgccgcatc cgacagctgc
     5881 cgccgccggc ggggcaggtg ccaatcatag cggtaacggc caatgcgctc gaaacggacc
     5941 ggcagaactg cctggcggcc ggcatggacg gctatctgaa aaaaccggta gacatcgaag
     6001 ccctcagggc gtcgctgcag ccttaccttg ccgtgcctgc gtatcctgcc ggggtcgctc
     6061 gggactagtg cgcaggatca gcagcgttct ggttttgtcc gatacatgtg ccgcatcgac
     6121 gtagccgatg gcagcggtgt cttcggacac tttccggatc atctccacca ccgaattcgc
     6181 gccggccggc agacggccgc gaccgctgaa caggtaatag gcgcggtagc gtttcagttt
     6241 ggtgccgtcc agctgcagca ggcggcgata gaaatcagca taggttgtgt gttcttcggg
     6301 caggtccagc ggatggattt cctgtccggt ctgtggaaac aggggcagtc ggccgagaaa
     6361 gatttttttc aggtcatcct cggacagttc cgtcagcgga ttctgcggat gcacaatgat
     6421 cgcgatgtcg gcgcgtgcgc tgccgctgcc cagcgccagc agcgcgagca ggagcaatat
     6481 tggaaccgcc tgtgtccgga atccggtcat tgtcagaaca ccagatcgag cacaaaggtg
     6541 gtgagggagg tgtactgtgt tgcttcgccg gtctgttgca gcatttgccc gctggagcca
     6601 taggcaaatt cgaagtactg ccattcccct ttcagcgaaa gattcgacag cagctcgtaa
     6661 cgcacgccca gggtccaggt attctgctcg tagttgagcc cgttcacgac cgccaggctc
     6721 gtggtgcgca aatcatccag taccgcgtaa tatccggcgg gcacgacggc gtagctggcc
     6781 tggatgtcat ccttcatctc gtcgttgaat ctgtcggtga actggcctgc cacacaatag
     6841 ggcgtgaact tgcccaggcg gtattgcagc gacgtgtacc agccgttggc gttgttggtg
     6901 tagccttcgt cgaggttctg gatatcgaag acttcgccga tcagcgccag gtcgccgtgc
     6961 tcccagcggg cgcccagggt ctggtagcgg taccaggcat catccgcgct gagcgaattt
     7021 gcgatatcgt caaacagggg atcgatgctg gcggcggcgc ggtatgcgga ttccgcttcc
     7081 tcataggcgt ccacttcgat atcggtccgg tcaaagatca gaccgtagcg cagggtgact
     7141 tcctctttct gcaccgtcag gttgccgccg gccatgggtt ccacttcgat attcagtgaa
     7201 tcgcgcgagc gctccatgcg ccctgccagc aactgcaggt cgatggtgaa atcacgccag
     7261 tcggtgatcc aggcaacatc agcgccatcg aaattgctga acggggagag gatgggcgag
     7321 tacacgtcaa ccggcggacg cacccagaca taggaatagc caacgtcgag ggtttccgag
     7381 tacagataat ggggcgtgcg catgcggccc aggcggacac gccattcgga atcgagctga
     7441 tagctcagga aaagccagtc cagttccggt tcgaattcag cggtgtcgtc ccagttgtgg
     7501 ccacggctta cgacctgcac ggtgagggaa aggcgttctg ccagattgcc gcgggcatgc
     7561 agccccaggg cactgtcgga ttcgtaactc cattcttccc cgttgtaatc catgtacgtg
     7621 taggcgctgt cttccacacg accggcgccc agggaggcga aaccggagaa cgaccagtcg
     7681 tcccccgcca gcgcaggcga gctcgccagc aggcccagca gcaggtgttt tcccttggtg
     7741 aacaaatggt tcgctttcga cattccctgt actcttgttc gtttacacaa tcacaatcaa
     7801 ttgctgcaac tggaaagtgg cgagcgagta taaaatgctc aataacggcc tgtgtagtca
     7861 gacacccgac ctatcctgtg tcgatttgta gcggtgcgac tgcccgccac tgcttgatgg
     7921 acgtataaaa agaatccgct atcgccatgg aacgtaacgc ccgcatccgc ttgcagatca
     7981 tgttgattgc cgttatcggc ggtctggcat ttctttccta tttattggtt aacgtggtgc
     8041 tggcagcggt gaacgcggat cgcctggacc gcctgcaaca tcatcactat cccatcatcg
     8101 aggaaatccg tctgctgcag caggatctga acagcatgcg ggaaggtttt gcaacggcgg
     8161 tgggtctgga agatcctgtg ctgctggagg atacccgtca ggtggtgcag cacgcgcgcg
     8221 cgcggttggc gcgattgcag acgcgcgacc cggatctgac ggatgaagtg gagggcatca
     8281 gcgcggcatt cgaagcctac gtgcagccag cgttgttgct gacggaagcg ctggtggcgg
     8341 acccgcgcca attgccgggc tacaagggtg cgatggaagc gtcgctgcgg gattaccagt
     8401 tgctgagcga ctggatcggc gggttgcatg cagcccgcca ggtggcgtat cgcaatctgt
     8461 tgcgtgaaac caatctggcg gtgaatcgcg ccaatctgtg gggtgctgtg ctgggcgcgc
     8521 tggtaattgt cgcgctgggc gtgctggcct gggcgatttc cgcgcgggtg ttgcgtgaaa
     8581 tcaaccgcag cgatcgtttg aaagatgaat ttctgagtgc catcagccac gagctgcgca
     8641 cgcccatgaa cggcatcatc ggtgcgctca gcctgctgcg tgccacgacg ctggaccggg
     8701 agcaggtgtc ctggctcgat gtggccaacc gttccgccag cagcatgatg agttccatcg
     8761 atgatctgct gcagttcagt gaactgatgg cggggcagga ggcattgcat gtgggcgagt
     8821 ttcgtctgcg cggtggcctg acccggttgc tggcggcgct gcggccggag ttcgaggata
     8881 aaaatctggc actggagttt tacggtggcg tggtgctgga ccgggtgctg gtcagccacg
     8941 aaggaaaaat tctctatgtg atccgccatc tgctgagcaa tgcgctgaag ttttccgatc
     9001 atggaccggt atgcctgtcg atcgggcagg cgaacaggtc ggcagaagtg ggcgagggca
     9061 gcatccgcgt gctggtgcag gattcgggac cgggaattcc ggagccactg ctggcggagc
     9121 tggggcgtcc gttccgtcag ctggatggtt ccttcagtcg ccgtcatggt ggcatgggca
     9181 tcgggctggc cacctgtcat gcgattgcgc acttgctggg tgccagtctg acgctgcgca
     9241 atcgcgagag cggcgggctg gaagtggaat tcatcgtgcc agtgaagttt ggcgatactg
     9301 cgccggaatc cgggcccgat tccacgacgc tgaccggtgg cagttatcgc cggtccggtc
     9361 tggtgctggt ggccgaggac aatctggtga acctgatggt gctgaagggt tatctgaagc
     9421 atctgggctg caatgtggtc agcgcgcgca acggctatca ggcgctggac ctgatgcaga
     9481 gcaaactggt ggatctggtg ctgatggatt gccagatgcc attgatggac gggctggagg
     9541 caacccgcca gatccgcaag ttacccgagc cgttgcgcga tacgccgatc gttgcgctga
     9601 cggcgaatgc caccgatgcc gatcgt
//
LOCUS       DASUEG010000202         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1748573, whole genome shotgun sequence.
ACCESSION   DASUEG010000202 DASUEG010000000
VERSION     DASUEG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748573"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFX11_03865"
     CDS             complement(<1..731)
                     /locus_tag="VFX11_03865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4937785.1"
                     /translation="MPSLNICICTCKRETLLVQCLESVARVEVPANVHVTVTVIDNDA
                     DRSAEPSVNALTRQFPFPLQYVCEPRRGIPLARNRALDETYRLGSDYLLFIDDDEWVR
                     PDWLRIIYEYAVAQNGNVIVSGEVIPELPDTTPPEIRGVYKIKNRKTGERVQSCATNN
                     VLVPVSMIRGWGLRFDESNPLAGGTDTIFFHEAVSRGAVIHKCAEALVHEAIPASRAN
                     LKWLAKRKYRAGITEAWRKQRKGRPR"
     gene            1024..>2267
                     /locus_tag="VFX11_03870"
     CDS             1024..>2267
                     /locus_tag="VFX11_03870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX4937786.1"
                     /translation="MKITVFGTGYVGLVTGACLADVGHDVLCVDVDESKIRKLREGII
                     PIHEPGIDEVVKRCVAEGTLKFTTSAQDAVTHGTLQFIAVGTPPDEDGSADLKYVLAV
                     AKTIGQYMTGYKVVVDKSTVPVGTAEKVKAAVLAQLAERGANLPVDVCSNPEFLKEGN
                     ALEDFTKGARIVVGTDSEQVVELMRECYAPYNRNHDKMMFMDIRAAELTKYAANAMLA
                     TKISFMNEMSQLAERLGVDIEQVREGIGSDPRIGYHFIYPGCGYGGSCFPKDVKALTR
                     TAQSIGYAAELLEAVDSVNERQKSILFDKLVKAFDGNLKGKTIAVWGLAFKPNTDDMR
                     EAPSRVLMEAVWAAGGRIQAFDPEAMQECARIYGTRPDLVLCENRKDAVAGADALVIC
                     TEWKTFRSVDFGWLKTQLKFPVV"
BASE COUNT          471 a          673 c          682 g          441 t
ORIGIN      
        1 cgcggtctgc ccttgcgctg cttgcgccat gcctcggtaa tgcccgcacg atacttccgc
       61 tttgccagcc acttcaggtt ggcacggctg gccggaatgg cctcgtgtac cagggcttcc
      121 gcacatttgt gaatcaccgc gccacgggac accgcctcgt ggaagaagat ggtgtcggtg
      181 ccaccggcca gcgggttgct ttcgtcaaag cgcaaccccc agccacggat catagacacc
      241 ggcaccaaca cgttattggt ggcacagctt tgcacgcgct caccggtttt gcggttcttg
      301 atcttgtaga caccacgaat ttccggcgga gtggtgtctg gcagctcggg aatgacctcg
      361 cccgatacaa taacgttccc attctgggcg acagcatact cataaatgat acgcagccag
      421 tctggccgta cccattcgtc atcatcgata aacagcaggt agtcactgcc cagccggtag
      481 gtttcatcca gagcgcggtt acgtgccagc gggatgccac gacggggttc gcagacgtac
      541 tgcaggggga acggaaactg ccgggtcagg gcattgacac tcggctctgc cgagcggtcg
      601 gcatcattgt caatgacggt aacggtaaca tgcacgttcg caggtacttc tacccgggcg
      661 acgctctcca ggcattgcac cagcaacgtc tcccgcttgc aggtacagat acagatatta
      721 agggatggca tcggcggcgt tcacctgttc cggcgagggt tggaccggcg gcaagtgtac
      781 accagcaggt gtcccagcgg aatgcacggc ttcggcggga aatttgcagg cattttcggg
      841 caatgaccca gctgatagat tttaggaccg atgccgttta ttctttgtcg gccagaggtc
      901 ccgtgtggca ccggttttcc aggaggaagt ccgggccatt ccgctcagga acgacgtcct
      961 gacacgacag cagcggtcgc tccgttcaat ttcaagtgca tcagtaaagg gaaaaccatc
     1021 gatatgaaga tcaccgtatt cggcaccggc tatgtaggac tggtcacggg cgcctgcctg
     1081 gcggacgtgg gccatgatgt tctgtgtgtg gatgtggatg aaagcaagat ccgcaagctg
     1141 cgggagggga tcatccccat tcatgaaccc ggtatcgacg aggttgtgaa acgctgcgtt
     1201 gcggaaggca ccctcaaatt taccacctcc gcccaggacg cggtcaccca tggcacgctc
     1261 cagttcatcg ccgtgggcac ccccccggat gaagatggct ccgccgatct caagtacgta
     1321 ctggcggtgg ccaagaccat cggccagtac atgaccggct acaaggtcgt ggtggacaag
     1381 tccacggttc cggtcggtac ggcggaaaag gtgaaggcag cggttctggc gcagctggcc
     1441 gagcgcggcg ccaacctgcc tgtcgacgtc tgctccaacc cggaattcct caaggaaggc
     1501 aatgccttgg aagatttcac caaaggcgcc cgcatcgtcg tcggcacgga ttccgagcag
     1561 gtggtggagc tgatgcgcga gtgctatgcc ccctacaacc gcaaccatga caagatgatg
     1621 ttcatggaca tccgcgcggc ggaactgacc aaatatgctg ccaacgccat gctggccacc
     1681 aagatcagct ttatgaacga gatgtcgcag ctggccgaac gcctgggcgt ggacatcgag
     1741 caggtccgtg aaggtatcgg ttccgacccc cgcatcggct atcacttcat ttatcccggc
     1801 tgcggctacg gcggctcctg tttccccaaa gacgtcaagg ccctgacccg taccgcgcag
     1861 tccattggtt acgcggcgga actgctcgaa gcagtggaca gcgtcaacga gcggcagaaa
     1921 tccatcctgt tcgacaagct ggtcaaagcc tttgacggca acctgaaagg caagaccatc
     1981 gcggtgtggg gcctggcgtt caaacccaac accgacgaca tgcgcgaagc tcccagccgg
     2041 gtgctgatgg aggcggtctg ggctgccggc ggtcgcatac aggccttcga ccccgaggcc
     2101 atgcaggaat gtgcccgcat ttacggcacg cgtccggatc tggtgctgtg cgaaaaccgc
     2161 aaagacgccg tggccggcgc cgatgcgctg gtgatctgca cggaatggaa aaccttccgc
     2221 tcggtggatt ttggctggct gaagacccag ctcaagttcc cggtcgt
//
LOCUS       DASUEG010000203         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1680524, whole genome shotgun sequence.
ACCESSION   DASUEG010000203 DASUEG010000000
VERSION     DASUEG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680524"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFX11_03875"
     CDS             <1..905
                     /locus_tag="VFX11_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937787.1"
                     /translation="QYLPLSLLLWLGLVAGVDARSPADSAPVALYSASLTPAAVAPAS
                     PANPVPDAPAIDWLALLDGTGVSEILDQANSLIGQEVANLEKAPLGFTAEELAALQMN
                     FRFRLGSERLKQDLLAQLQQKLPPDQAHHLQKLLQSPRVQFLQTLQAQLNDEVVREAM
                     RSYRVQVQESAPSANRVELVSTLDQTLRQSALEADLKVELRKQLLVTVSQLKSHETIP
                     ESMLDEQLRPYRQDVEQQISQNALHAYLYLFKRTPSPQLQDLISSLDQPAFDSFMSIC
                     QSTLLDSFRAARAQLQQDMRLARQ"
     gene            933..1628
                     /locus_tag="VFX11_03880"
     CDS             933..1628
                     /locus_tag="VFX11_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4937788.1"
                     /translation="MSLTVRNARLLLVEDHRALAETILDFLQTVGFETDYAADGLTGL
                     HLACHHHYDLIVLDVMLPGLNGFELCARLRNEKAITTPVLMLTARDQLQDKLEGFQHG
                     ADDYLVKPFDLPELVARLVALLRRSRGEVAQDILQVADLTFHTGTLEVKRAGQTLHLS
                     PTCLRILKILMRESPRVVTREALEQELWGDLLPDSDTLRSHLYKLRRTVDKPFAQPLL
                     HTLAGQGFKLAPE"
     gene            complement(1609..2946)
                     /locus_tag="VFX11_03885"
     CDS             complement(1609..2946)
                     /locus_tag="VFX11_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4937789.1"
                     /translation="MQRPRVNTKLLKIFLFQVILISAAASIGIYSAALVAEDLLVNEA
                     LAGEADYFWQRYQQDSNASLPDTLNLLGYLHDPIAGHHAPDWLLNLPAGMQRAQHEGQ
                     QPIVHVSENAGRTLYLVFDEEQVSRLSFYFGIVPLAVVLLVLYALSYLTYLQAKRAIS
                     PIVQVAAQMAHFDINRTELPQPDWTSVRRIADAETAALVDAIDAFLARMHKFVARERN
                     FTRYASHELRTPLAVIKGSLANLQALSGLDEQPPPNMSAHTITISAEKMQRQLQRMDT
                     TVKDMEALLETLLLLAREDEQLGSSDSILVNDVAALLMEPLHLQNTRPQVRIELQHNG
                     FLSTRAPQRLVSIVLTNLLRNALTYTKEGQVTLTIDENGFSVEDTGIGIPPHELASIF
                     DPFFRSSGNQEKGFGLGLAIVQKICAQLGWTIAVKSTAGSGTRFDVRVTPAPV"
     gene            3104..>3525
                     /locus_tag="VFX11_03890"
     CDS             3104..>3525
                     /locus_tag="VFX11_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX4937790.1"
                     /translation="MSRPIFKTTISGVVLLSAIALGGCDDDDNNTGAGGMRAVSPAAD
                     GVARLRVFHAVADAPNVNVLANGNAVLTNVPYGAASALVGVNPATYNVQVDGILPGGT
                     ATVIGPAGIPLADADITNVLAVNTLAAIEPLVVTVPDET"
BASE COUNT          674 a         1033 c         1087 g          731 t
ORIGIN      
        1 ggcagtatct gccgctgagt ctgctgctat ggctgggact ggtggcgggt gtcgacgcgc
       61 gttcccccgc cgattcagcc ccggttgcac tctattccgc cagcctcaca cccgccgccg
      121 tcgccccagc ctcccctgcc aatcccgttc cggatgcacc tgccatcgat tggcttgccc
      181 tgctcgatgg taccggtgtc agcgaaatcc tcgatcaggc caacagcctg atcgggcagg
      241 aagtggcgaa cctggagaag gcaccgctgg gctttacggc agaggaactg gcagccctgc
      301 agatgaattt ccgttttcgc ctgggcagcg agcggctgaa gcaggatctg ttggcgcaac
      361 tgcagcagaa gctgcccccg gatcaggccc accatttgca gaaattgctg caatcccccc
      421 gcgtgcagtt tctgcagacc ctgcaggcgc aactgaatga tgaagtggtg cgcgaggcga
      481 tgcgatccta ccgggtgcag gtgcaggaat ccgcgcccag tgcaaaccgg gttgaactgg
      541 tgtccacgct cgatcagacc ttgcgccagt cggcgctgga agccgatctg aaagtggaat
      601 tgcgcaaaca actgctggta accgtgagcc agctgaaatc ccacgaaacc attcccgaat
      661 ccatgctgga tgagcagttg cgtccctacc gccaggatgt ggaacagcag atcagccaga
      721 atgcgttgca tgcctatctt tacctgttca agcgcacgcc cagcccgcaa ttgcaggatc
      781 tgatcagctc cctcgatcag cccgccttcg acagcttcat gtccatctgc cagtccacgc
      841 tgctggattc cttccgcgcc gcccgtgcgc agttgcagca ggacatgcgc ctggcccgcc
      901 aatagcctcg ctgcaatggg tatcgcatgc tgatgagtct caccgtcaga aacgcccggc
      961 tgctgctggt ggaagatcac cgcgcactgg cggaaaccat tctcgatttt ctgcagacgg
     1021 tgggctttga aacggattat gcggcggacg ggctgaccgg cttgcacctg gcctgccatc
     1081 atcattacga cctgatcgta ctcgacgtga tgttgcccgg gctcaatgga tttgaattgt
     1141 gcgcacgttt gcgcaatgag aaggcaatca ccacgccggt gctgatgctg actgcccgcg
     1201 atcaattgca ggacaagctg gaaggcttcc agcatggtgc tgacgactat ctggtgaagc
     1261 cctttgatct gccggaactg gtggcgcggc tggtcgcgtt gctgcggcgt tcacggggtg
     1321 aagtggcaca ggatattctg caggtggctg atctcacttt tcataccggt acgctggagg
     1381 tcaagcgtgc cgggcagacg ctgcaccttt cacctacctg cctgcgcatt ctgaaaatcc
     1441 tgatgcggga atctcccagg gtggtaacgc gggaggcgct ggagcaggaa ttatggggcg
     1501 atctgctgcc ggacagcgat acgctgcgca gccatctcta caaactgcga cgcacagtgg
     1561 acaagccttt tgcccaaccc ctgttgcaca cgctggcagg tcagggcttc aaactggcgc
     1621 cggagtgacg cgcacgtcaa aacgggtgcc gctgccggcg gtgcttttca ctgcaatggt
     1681 ccaccccagc tgcgcgcaga ttttctgcac gatggcaaga cccagaccaa accctttttc
     1741 ctgattgccg ctgctgcgga aaaacggatc aaagatgctg gccagttcgt gcggcggaat
     1801 gccgatgccg gtgtcttcca cgctgaatcc attctcatcg atcgtcagtg tcacctggcc
     1861 ttcctttgtg taagtgagtg cattgcgcag caggttggtg agtacgatgc tcaccagtcg
     1921 ttgcggtgcg cgcgtgctca ggaatccatt gtgctgcaat tcaatgcgga cctgggggcg
     1981 cgtattctgc agatgcaggg gttccatcag caatgctgca acatcgttca ccaggatgct
     2041 gtcactgctg ccgagctgtt cgtcttcccg cgccagcagc agcaaggttt ccagcagcgc
     2101 ttccatgtct ttcacagtgg tatccatgcg ctgcagctgg cgctgcattt tttcggcact
     2161 gatagtgatc gtgtgtgcag acatgtttgg tggtggctgt tcatccagtc ctgacagggc
     2221 ctgcagattt gccagtgaac ccttgatcac cgccagtggc gtgcgcagtt catggctggc
     2281 gtagcgggtg aaattgcgtt cgcgcgccac gaatttgtgc atgcgggcaa gaaaggcgtc
     2341 gattgcgtcc accagggcgg cggtttccgc gtcggcgata cgacgcacgg acgtccagtc
     2401 cggctgtggc agctcggtgc ggttgatatc gaaatgcgcc atctgtgccg ccacctgcac
     2461 gatcggcgat atcgcgcgct tggcctgcag ataggtgaga taggaaagcg cgtacagcac
     2521 cagcagcacc accgccagcg gcacgatgcc gaaatagaac gacaatcgcg atacctgctc
     2581 ctcgtcgaat accagataca gggtgcgacc cgcattttcc gatacgtgga cgatgggttg
     2641 ctggccttcg tgctgggccc gctgcatgcc ggcgggcagg ttgagcagcc agtccggtgc
     2701 atggtgaccg gcgatcggat cgtgcaggta gcccagcagg ttgagtgtgt ctggcaggct
     2761 ggcgttggaa tcctgctggt agcgctgcca gaaataatcg gcctcaccgg ccagtgcctc
     2821 gttcaccagc agatcctcgg ctaccagcgc ggcggagtag atgccgatgc tggcagcggc
     2881 gctgatcagg atcacctgga acagaaaaat cttcagcagc ttggtgttga cgcgtggacg
     2941 ttgcacagca cgacttcctg gcaggggtgg aataccgatc ctatcaggct agacaaatgg
     3001 ctgtgaacgt tgcgtgaacg tccggccgga aacgttcacc gcctgctcac ggacggaacg
     3061 ctagagtggc gtctcccaca aaaacaggat cgaggagatg atcatgagca ggcccatttt
     3121 caagacaacc atttccggag tggtgctgct gtcggccatt gcgctgggcg gttgtgacga
     3181 cgatgacaac aacaccgggg ccggtggcat gcgcgcagtg tcgcctgctg ccgatggtgt
     3241 ggcccggttg cgggttttcc acgcggtggc ggatgcgccc aacgtcaatg tgctggccaa
     3301 tggcaatgcg gttctgacca atgtgcccta cggtgcggcc agtgcgctgg tcggcgtgaa
     3361 cccggcgacc tacaacgttc aggtggacgg tattttgccg ggcggtacgg cgacggtcat
     3421 tggtccggcg ggcattccgc tggccgacgc tgacatcacc aacgtgctgg cggtcaacac
     3481 gctggctgcc atcgagccgc tggtggtgac ggtgccggat gaaac
//
LOCUS       DASUEG010000204         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2105288, whole genome shotgun sequence.
ACCESSION   DASUEG010000204 DASUEG010000000
VERSION     DASUEG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105288"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFX11_03895"
     CDS             <1..653
                     /locus_tag="VFX11_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937791.1"
                     /translation="AWHLLHEPTAVARQLEPLPAWPVAALENVSAAELFGADPAAVLV
                     GCNERPCRLVAPRLGMMQEHDHSEHQSSRSQFALTDVQAEEGNQSDLNAAAVAAARNA
                     LGLSPDVPAASVATITVFEGEYGFLNKRLPVLKVTFASADHATVYWEPATGAVAAVLR
                     DRDRYEGYSFGFLHKYHWLDAAGKNVRDAVMVLVALLVATVTVLGCWMAWRRRGNL"
     gene            729..1334
                     /gene="cobO"
                     /locus_tag="VFX11_03900"
     CDS             729..1334
                     /gene="cobO"
                     /locus_tag="VFX11_03900"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4937792.1"
                     /translation="MDQDKKERHNERMQRKKAVVDAGIEKAQEVRGIFIVITGNGKGK
                     STSAFGTLFRALGHGHQVGVVQYIKGTQSSGEVLYLQQHTPNLRYHAMATGFTWNTQN
                     WDADKKAADAAWEQSRLMLQDASLNLVVLDELTYMLKYNYLDTQEVLEAIRNRPPLQT
                     VIITGRAASPELIELADTVSEIRDIKHAFQAGVKAQPGVDF"
     gene            complement(1365..1790)
                     /locus_tag="VFX11_03905"
     CDS             complement(1365..1790)
                     /locus_tag="VFX11_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007624025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX4937793.1"
                     /translation="MKIISCLLLGTLLTLAGCQSLNASATLYDELGGMPVIEKITDNF
                     INEISFDQQIAAHFRETKIERFREKLIEQLCNVSDGPCKYTGDSMLDVHQKMNITEGE
                     FNRTVDLLINAMNQAGVAHRTQNKLIARLAPMRPDIIYH"
     gene            complement(1796..2641)
                     /locus_tag="VFX11_03910"
     CDS             complement(1796..2641)
                     /locus_tag="VFX11_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HEX4937794.1"
                     /translation="MAKCKGIATLLLCAPAALAWAGSGKLQETAGVTQIEGSSGGGLV
                     PWATLAGYDSREETAASVFATRVSTDDYRLHSWGGAFSWHDRVELSATHQTFDLTATA
                     FEISQNVIGAKVRVYGDVIYSRWPQLSIGVQYKELLDTTLAEAVGAKDFDNGTDVYLA
                     ATKAHLGALFGYNLVWDLTLRGTKANQFGLLGFGGDQNDSYEIMGEGSLAILFSRSLA
                     LGVEYRQKPDNLGFAEEEDAADIFVAYIPNKQFNLTVAWVDLGSIAGSDQQTGPYVSL
                     TGYLW"
     gene            complement(2709..>4669)
                     /locus_tag="VFX11_03915"
     CDS             complement(2709..>4669)
                     /locus_tag="VFX11_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4937795.1"
                     /translation="PAMDMVQSTLNAGSATAVVQLDTDLYQVIMLPVKVPVPVALTVI
                     GFRINEALAQELRQVTHLDVTFAAESTSSAPVRISTMPRDEMENALATSDAELNRRLP
                     FLSQLRHTTKRYPLSEQNRIYVYLSTAVDSAFARFDELQLEIILISLVAIGLSLLGGA
                     LVARNLTRPLRMLAELAAEIAAGHYRRDVQVSRNVREIGDLMSAFNTMQADLSEREAR
                     IIYQAHHDPLTGLINRQHVLTLLDDMLQQPDAPALLVACLNILDFRIVNDTFGHNVGD
                     SCLKQISTRLQELGGEQHLAARLGGDEFLFIAPQKEMATTANNILTALQQPYSSHELD
                     INLHFSMGVAASPQDARSANALVNKASIALDLSRRQRQGIVYYERKMEEAYLKRLTML
                     ADLKAAIQANDGQLKMYYQPKVCARTLTVSRFEALIRWIHPHHGFVPPDQFIPLAEQA
                     GLINALTEWVVGAVIRQISDWQQQDFTAQVAVNLSAQDLSREQLLEHVNNLLQRHGVA
                     ASALSFEITESELMRDAKAAIQLLEKFRGQGFHLAIDDFGTGYSSLSQLKNMPVTELK
                     IDRSFVMQLDTLEDDQIIVKSTIDLAHSFSLEIVAEGVENQAALQLLQQWGIDWIQGY
                     YFSRPLPAGEVLPWVLQFTASQVISATR"
BASE COUNT          882 a         1350 c         1449 g          988 t
ORIGIN      
        1 gtgcctggca tctgttgcat gaaccaactg cggttgcccg tcagctggaa ccgttgccgg
       61 catggccagt ggcggcgctg gaaaatgtgt ccgcagccga actgttcggc gctgatccgg
      121 ctgctgtgct ggtgggatgc aatgagcgcc catgccgcct cgtcgcgccc aggctgggca
      181 tgatgcagga acatgatcac agtgaacatc aaagttcccg cagccagttt gccctgaccg
      241 acgtgcaggc tgaggaaggc aaccagtctg atctgaatgc ggctgccgtg gccgcggcac
      301 gcaacgcgtt ggggctgtcg ccggacgttc ccgccgccag cgtcgccacc atcactgtgt
      361 tcgaaggcga gtacggtttt ctcaacaagc ggctgccggt gttgaaggtg accttcgctt
      421 ccgccgatca cgccaccgtt tattgggagc cggccactgg agcggtggcc gccgtgctgc
      481 gcgatcgcga ccgttatgaa ggttacagct tcggtttcct gcacaaatat cactggctgg
      541 atgcggccgg caagaatgtg cgcgatgcag tgatggtgct ggtagccctg ctggtggcga
      601 ctgtcaccgt gctgggctgc tggatggcgt ggcgccgtcg cgggaatctg taaaccgttc
      661 catgacggcc gaatttttcc ggtacactac cggcgatttt tattcgccat caaccgggag
      721 cggccacaat ggaccaggac aagaaagaac gtcataacga gcgcatgcag cgcaagaaag
      781 cggtggtgga tgccggtatc gaaaaggcac aggaagtgcg cggaattttt atcgtgatca
      841 ccggcaacgg caagggcaag tccacctcgg ccttcggtac cctgtttcgc gccctgggtc
      901 atggccatca ggtgggggtg gtgcagtaca tcaagggtac ccagtctagc ggcgaggtgc
      961 tgtatctgca gcagcacacg ccgaatctgc gctatcacgc catggctacc ggcttcacct
     1021 ggaataccca gaactgggat gcggacaaga aggccgccga tgcggcatgg gagcagtctc
     1081 ggctaatgct gcaggatgcc agcctgaacc tggtggtgct ggacgaactg acctacatgc
     1141 tgaagtacaa ctatctggac acgcaggagg tgctggaggc gattcgcaac cgtccgccgc
     1201 tgcagacggt gatcatcacc ggccgtgccg ccagccccga actgattgaa ctggcggaca
     1261 cggtgagcga gatccgcgat atcaagcacg cgttccaggc cggcgtgaag gcgcagcccg
     1321 gcgtggattt ctgatccgcg actttacagg ccagaaatga tggctcagtg atagatgatg
     1381 tccggccgca tcggcgccag ccgcgcaatc agtttgttct gcgtgcggtg cgcgacgccg
     1441 gcctgattca tcgcattgat cagcagatcc acggtgcggt tgaattcgcc ttcggtgata
     1501 ttcattttct ggtgcacgtc cagcatgctg tcgcccgtgt atttgcaggg gccatcgctg
     1561 acattgcaca actgttcgat cagcttttcc cggaaacgct caatcttggt ttccctgaaa
     1621 tgcgccgcga tctgctgatc gaaactgatt tcattgatga agttgtcggt gattttttcg
     1681 atcaccggca tgccgcccag ttcgtcgtac agtgtggcgg aggcattcag gctctggcag
     1741 ccggccagtg taagcagcgt acccagtaac aggcaggaaa tgattttcat ggcggtcacc
     1801 acagatatcc ggtgagggaa acgtagggtc cggtctgctg gtcggaaccg gcgatggagc
     1861 ccagatccac ccaggcgacg gtgagattga actgtttgtt gggaatgtag gcgacaaaaa
     1921 tatccgctgc gtcttcttcc tctgcaaagc ccagattgtc gggtttctgc cggtattcca
     1981 cgcccagtgc cagcgagcgg ctgaacagaa tcgccagcga tccctcgccc atgatttcgt
     2041 agctgtcgtt ctggtcgccg ccgaaaccca gcaggccgaa ctggttggcc ttggtgccgc
     2101 gcaacgtcag atcccacacc aggttgtagc cgaacagcgc gcccagatgt gccttggtgg
     2161 cggccagata aacatccgtg ccgttgtcga aatccttcgc gcccaccgct tctgccagcg
     2221 tggtatccag cagttccttg tactgtacgc cgatgctcag ctggggccag cgcgaataaa
     2281 tcacgtcgcc gtacacgcgt actttcgcgc cgatgacatt ctggctgatt tcaaatgcag
     2341 tggcggtgag atcgaaggtc tggtgggtgg cagacagttc aacccggtca tgccagctga
     2401 aggcgccgcc ccaggaatgc aggcggtaat cgtcggtgct gacacgggtg gcgaacacgc
     2461 tggccgccgt ttcctcgcgg ctgtcatagc cggccagagt ggcccagggc accaggccgc
     2521 cgccgctgct gccttcgatc tgggtgacgc ctgccgtttc ctgcaatttg ccgctgccgg
     2581 cccaggccag cgctgccggt gcgcacagca gcagcgtggc aattcccttg catttcgcca
     2641 tggtattgct ccgtggaggg aggtggattc gttttgagta taggcggtgg ccaggattaa
     2701 cagaggcttt agcgggtggc gctgatgacc tggctggcgg tgaactgcag aacccagggc
     2761 agcacttcgc cagcgggcag ggggcggctg aagtagtagc cctgaatcca gtcgatgccc
     2821 cattgctgca gcaattgcag ggccgcctgg ttttccacgc cttcggccac gatttccaga
     2881 ctgaaactgt gggcgaggtc gatggtggat ttgacgatga tctggtcgtc ttccagggtg
     2941 tcgagctgca tcacgaagct gcggtcgatt ttcagttccg tcaccggcat gtttttcagt
     3001 tgcgacaggg atgaatagcc ggtgccgaaa tcgtcaatgg ccagatgaaa accctggccg
     3061 cggaattttt ccagcaactg gatcgccgct ttcgcatcgc gcatcagttc gctttcagta
     3121 atttcgaatg acagcgcgct ggccgctacg ccgtggcgct gcaacaggtt gttgacgtgc
     3181 tccagcaatt gctcgcgcga cagatcctgc gccgacagat tcaccgccac ctgggcagtg
     3241 aaatcctgct gctgccagtc gctgatctgc cggataacgg ctcccaccac ccactccgtc
     3301 agcgcattga tcaggcctgc ctgttccgcc agcggaatga actggtcggg cggaacaaag
     3361 ccgtggtggg ggtgtatcca gcgaatcagt gcttcgaagc gcgagacggt cagggtgcgc
     3421 gcgcagactt tcggctgata atacattttc agctggccat cgttggcctg gattgccgcc
     3481 ttcaggtctg ccagcatcgt cagccgtttc aggtaagcct cttccatctt gcgttcgtaa
     3541 tagacaatgc cctggcgttg ccgccgtgac agatccagtg cgatgctcgc cttgttgacc
     3601 agcgcattgg cgctgcgcgc gtcctgcggg ctggcggcca cgcccatgga aaaatgcaga
     3661 ttgatgtcca gctcgtggct ggaataaggc tgctgcaacg ccgtcaggat attgttggca
     3721 gtggtggcca tttccttttg cggtgcgatg aaaagaaatt cgtcgccccc caggcgggcg
     3781 gccagatgct gttcaccgcc cagttcctga agccgcgtgc tgatctgttt caggcagctg
     3841 tcacccacat tgtggccgaa ggtatcgttg acgatacgga aatccagaat gttcaggcag
     3901 gccaccagca gtgcgggcgc gtccggttgc tgcagcatgt catccagcag cgtgagtacg
     3961 tgctggcgat tgatcaggcc ggtcagggga tcgtgatgcg cctggtagat gatgcgcgcc
     4021 tcccgttcgc tcaggtctgc ctgcatggtg ttgaacgcgc tcatcagatc gccgatttcg
     4081 cgcacgttgc ggctcacctg cacgtcgcgc cggtaatgtc cggcggcaat ttctgccgcc
     4141 agttccgcca gcatgcgcag tggccttgtc aggttgcggg caaccagcgc gccacccagc
     4201 agcgacaggc caatggccac cagactgatc aggatgattt ccagctgcag ttcatcaaaa
     4261 cgggcaaagg ctgaatccac tgccgtggac agatacacat agatgcggtt ctgctccgac
     4321 agcggatacc gcttcgtggt gtgccgcaac tgcgacagga agggcaggcg ccgattcagt
     4381 tccgcatcgc tggtggccag cgcattttcc atttcgtccc gcggcattgt cgaaatgcgc
     4441 acgggggcgc tgctggtgct ttccgccgcg aaggtgacat ccagatgcgt gacctgcctg
     4501 agttcctgcg ccagcgcttc gttgatgcgg aatccgatga cggtaagcgc cactggcacc
     4561 ggcactttca ccggcagcat gatcacctga tacagatcgg tgtccagctg caccacggcg
     4621 gtggcgctgc cggcgttgag tgtggattgc accatgtcca tggcgggaa
//
LOCUS       DASUEG010000205         7036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_71849, whole genome shotgun sequence.
ACCESSION   DASUEG010000205 DASUEG010000000
VERSION     DASUEG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71849"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..356)
                     /locus_tag="VFX11_03920"
     CDS             complement(33..356)
                     /locus_tag="VFX11_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937796.1"
                     /translation="MGLLNKIELLKDMIQTAIDNGARTVEDVHKSIADLPFEVLEKNG
                     LLDEDGQQLRERNQQTIGAVYGKIREVNQKIGELASDMFESLEDGQHIARVMDEKSNS
                     KTTDP"
     gene            complement(380..1519)
                     /locus_tag="VFX11_03925"
     CDS             complement(380..1519)
                     /locus_tag="VFX11_03925"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX4937797.1"
                     /translation="MTIAANPNPEQLQHVPGALTGNAPRTRPSKETLDALQKELDELR
                     DSVRADLGQRDADYIRNIIRIQRLCEIAGRTLIHFSFTPIPFLLGVTALSVAKIMDNM
                     EIGHNVMHGQYDWMNDPKIHSTNFDWDTACDNKSWMRTHNFEHHTFTNILNKDRDFGY
                     AVLRLSDDTPWHPLRSIQIIYFGLLSTFFQWGVALHELESEKLREGTFDLEEKKPYIK
                     AMLKKAGRQVFKDYIFFPALAGPFFWKVALGNGMANLARNLWASSIIFCGHFTEHTQT
                     FTEEECENESRGHWYYRQMLGSSNFDGNRLLHIMSGHLSYQIEHHLFPDIPAHRYAEM
                     SPQVREICERHGLHYNTAPFAKQYASVIKRVLKFSLPSSLRKLTA"
     gene            complement(1534..2667)
                     /locus_tag="VFX11_03930"
     CDS             complement(1534..2667)
                     /locus_tag="VFX11_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX4937798.1"
                     /translation="MESNKPTLASLTRSWFNLSLFNSRRAGDYFEYLLQGIDPMWSAT
                     ECRARVLEVRDETADTKTFVLRPNATWKGFQAGQHLHISMEINGARQTRTFTISSLPQ
                     EWEQQGFITLTVKKVPDGRVTPWMHEHLLPGTVVSISQAGGDFVLPTDLNESLAFIAA
                     GSGITPIASMLRSLVARNMPVPSSLLYFARTDQDFIFGDTLQGLSRAMSRFSLHLIAA
                     DGGSKKKGRSTLPQGFISAEHVRAVLTGEPKTIYVCGPHPFRELAKQLLAEQGFPLNQ
                     IREEAFGLPPLPRTEGAPVTVSFSRSHTSTTTDQPGTLLEMAEQSGLKPTSGCRMGIC
                     YTCKCTKKSGQVRNVITGEISSNDEEDIQICVTTPVSNVELAI"
     gene            2936..3565
                     /locus_tag="VFX11_03935"
     CDS             2936..3565
                     /locus_tag="VFX11_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4937799.1"
                     /translation="MNNRQERKLLTRQSLMNAALQWVEEGQHISSLSIREVAKRAGVV
                     PTAFYRHFSDVDDLTLNLVDELSKVLRQLMREVRQNRTDPQALIQDSIARYCDYAFRQ
                     RVYFVFLTQSMTGGTPLIRQAIRSELQYFANELATDVRHLGLLPNMEQTMLENVALLT
                     INTVANATMELLDLPDGNGAARQQFEKRLKRHLQVIFIGAAHWKTAAPG"
     gene            3694..6432
                     /locus_tag="VFX11_03940"
     CDS             3694..6432
                     /locus_tag="VFX11_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4937800.1"
                     /translation="MRVNMPVTRNEIKLAPGQTIVSKTTPKGVITYVNRDFVEISGFS
                     EPELIGQAHNLVRHPDMPQAAFKDLWDTLQAGKPWRGMVKNRCKNGDYYWVEANVTPI
                     WSGGRVTEYMSVRTAPSQQQIDDAEALYARMNAGEKVRPSLAARGERYFRNLPVNGKL
                     GWLVGVFALVLLGDFLLSGEALPWPYIMTLLIACGALFQFVQDVVKKPMQEAAVALRK
                     FSSGDFDTHLNILRDDEPGQVLQGMQCMKIKLNFELENTRLIANEALRVKNALDVCDT
                     NVMLADPDYNIIYMNDSLRKMMKNAEADLKTVLPAFDAEKLLGCNMDMFHRDRTHQRR
                     ILDNAKSTHRQEVVVAGRTIVITATPVFDQGGKRLGTVIEWLDRTEELRKAAEQQRIE
                     QGRLDAERRVANENARIRQALDSVSANVMVADNDLNVVYTNHAVISMMRKAERDIQTE
                     LPNFHVDKIMGSNIDIYHKNPSHQRQMLKNLKQPVKSVFLLGGRTLQVYASPVTDSHG
                     ESIGTVVEWTDRTEEVAIEKELDHMIRSAAIGNLSSRATVSGRAGFFLTMSQGINQLL
                     EICEDVVSEMVAVMERLAHGDLRAKINKEYQGEFGRLKDNVNETVDRLVDIVTEIRTA
                     SSAVLNGADEIAQGNADLSQRTEEQASSLEETASSMEEMTSAVRQSADNARRVEQLTA
                     ATRENAQKSGEVAQQAVGAINAINVSSKKIADIIGVIDEIAFQTNLLALNAAVEAARA
                     GEQGRGFAVVAGEVRSLAQRSANAAREIKALITESVTRVEDGTALVRESGNMLNQIIQ
                     SVNDVSGLISELAQSAQEQSSGIDQVNTAVAQMDEMTQQNAALVEEASAASEAMAEQA
                     RKLGEQIAFFRLDDTERTPARRAAPVASGGRAAAPRAVAQPPRSRGHQSSDEWEEF"
     gene            complement(6536..>7036)
                     /locus_tag="VFX11_03945"
     CDS             complement(6536..>7036)
                     /locus_tag="VFX11_03945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4937801.1"
                     /translation="ATDQLTGLKNRRYLDNTLAEEFARCARYGHTLSVLLMDIDHFKR
                     FNDTYGHLIGDECLKDVAHSVRKGLRWPSDRAARYGGEEFCVILPETETEGALTVAER
                     IRRNVELMNFMVKDQRVPVTVSIGLAVIKPQPHQKPELVLEFADSALYLSKEAGRNRV
                     TLHNQN"
BASE COUNT         1450 a         1901 c         2223 g         1462 t
ORIGIN      
        1 cggccctgca aggaccggcc gccatggggg gcttatgggt ctgtggtctt gctgttggac
       61 ttttcgtcca ttacgcgagc aatatgctgt ccgtcttcga gggactcgaa catatcagaa
      121 gccagctcac caatcttttg attaacttcg cgaatcttgc catatacggc tcctatggtc
      181 tgttgattgc gttcgcgtaa ctgctggccg tcttcatcca ggagtccatt cttctccagt
      241 acctcgaaag gcaggtcggc aatggacttg tgcacgtctt ccactgtgcg ggcgccattg
      301 tcgatagcgg tctggatcat gtctttcagc agttcgatct tgttcagaag tcccatgttc
      361 agtcctcggc cggggctgct caggccgtca gcttgcgcaa tgacgacggc agcgagaatt
      421 tcagcacccg ttttatcacg ctcgcgtact gcttggcgaa tggcgcggtg ttgtagtgca
      481 ggccgtgacg ttcgcagatt tcccgcacct ggggcgacat ttcagcgtac cggtgggccg
      541 ggatatccgg gaacaggtga tgctcgatct ggtagctcag gtggccactc atgatgtgca
      601 gcagacgatt gccgtcgaag ttgctggagc ccagcatctg gcggtaatac cagtggccac
      661 gggattcatt ctcgcactct tcctcggtaa aagtctgggt gtgttcggtg aagtgaccgc
      721 agaaaatgat gctggacgcc cacaggttgc gcgccagatt ggccatgccg ttgcccagtg
      781 ccactttcca gaagaagggg ccagccagcg ccgggaagaa aatgtagtct ttgaacacct
      841 gacggccggc ctttttcagc atggccttga tatagggctt tttctcttcc agatcaaaag
      901 taccttcccg cagcttttcc gattccagtt catgcagcgc cacgccccac tggaagaacg
      961 tgctgagcag gccgaagtaa ataatctgga tgctgcgcag cgggtgccac ggagtgtcat
     1021 ccgacagacg cagtacggcg taaccgaaat cgcggtcctt gttcaggata ttggtgaagg
     1081 tgtggtgctc gaaattatgg gtgcgcatcc acgacttgtt gtcgcaggca gtatcccagt
     1141 cgaaattggt tgaatggatt ttcggatcgt tcatccagtc gtactggccg tgcatcacgt
     1201 tgtgtccgat ttccatgttg tccatgatct tggcgaccga cagcgcggtg acgcccagca
     1261 gaaatggaat cggtgtgaag ctgaaatgga tcagggtgcg gccggcgatt tcgcacaggc
     1321 gctggatgcg gatgatattg cggatgtagt ccgcgtcccg ctgacccagg tcggcgcgta
     1381 cgctgtcgcg cagttcgtcg agttcctttt gcagcgcgtc cagggtttcc ttgctgggac
     1441 gggtgcgggg agcgttgccg gtcagggcgc cgggtacgtg ttgcagctgt tccggattcg
     1501 gattggcggc gatggtcatg gggaactcct ctctcagatg gccagttcga cgttgctgac
     1561 gggggtggtg acgcagatct ggatgtcttc ttcatcattg gaagaaattt cacccgtgat
     1621 cacattgcgt acctgtccgg attttttggt gcatttgcag gtgtagcaga tacccatgcg
     1681 gcaaccgctg gtgggcttca ggccactctg ctccgccatt tccagcaagg tgccgggctg
     1741 gtcggtggtg gtactggtgt ggctgcggct gaaactgaca gtgacgggtg cgccctcggt
     1801 gcggggcagc ggcggcaggc caaaggcttc ctcgcgaatc tgattcaggg ggaagccctg
     1861 ttccgccagc agttgcttgg ccagttcccg gaagggatgc gggccgcaga cataaatggt
     1921 tttcggttcg cctgtcagta ccgcgcgaac gtgctcggcg ctgatgaaac cttgcggcag
     1981 ggtggagcgg ccttttttct tgctgccacc atcggcggcg atcaggtgca ggctgaagcg
     2041 cgacatggcg cggctgaggc cctgcagggt gtcgccgaaa atgaaatcct gatcggtgcg
     2101 ggcaaaatac agcaggctcg aaggcactgg catgttgcgg gccaccagac tgcgcagcat
     2161 ggatgcaatc ggtgtgatgc ccgagccggc ggcaatgaag gccagggatt cgttcaggtc
     2221 ggtgggcagc acaaaatcgc cacccgcctg ggaaatgctg accacggtgc ccggaagcag
     2281 atgctcatgc atccaggggg tgacacggcc gtcaggcact ttcttcacgg tcagggtgat
     2341 gaagccctgc tgctcccatt cctggggcag cgaggaaatg gtgaaggtgc gggtctggcg
     2401 tgcgccattg atttccatgc tgatatgcag atgctggccg gcctggaagc ctttccaggt
     2461 ggcattgggg cgcagcacga aagttttcgt atcggcggtt tcatcgcgga cttccagtac
     2521 ccgggcccgg cattcggtgg ccgaccacat ggggtcgatg ccctgcagca ggtattcgaa
     2581 atagtcgccg gcgcggcggc tgttgaacag gctgaggttg aaccaggatc gggtcaggct
     2641 ggcaagagtc ggtttgtttg attccatggc gatggctccc gttgatttgc gagtacagtt
     2701 gtacaccaaa atgaaaatgc acgtcaacga gtgtgccgat taattttgta ctacaaatat
     2761 aaaaatatta aaaaacaaat agttatgatt tattaatttg tgttcaagtg tacactttgt
     2821 gagtatggat gccgtgtttc agggccttgt ttgggagcag ccggcgcgtc aatgtggcac
     2881 ttccaggtct aatctgcgta caattgcgca ccgatttgaa ccgggccgga accagatgaa
     2941 caaccgtcag gaacgcaagc tgcttacccg ccagtccctc atgaatgccg cgctgcagtg
     3001 ggttgaggag gggcagcata tttccagcct cagcatccgc gaggtggcca agcgtgctgg
     3061 cgtggtgccc acggcgttct accggcattt cagcgatgtg gatgacctga ccctgaatct
     3121 ggtggacgaa ctcagcaagg tgttgcgcca gctgatgcgg gaagtgcgcc agaatcgcac
     3181 cgatccccag gcactgatcc aggattccat tgcccgttac tgcgactatg cgttccgcca
     3241 gcgtgtgtat tttgtatttc tcacccagtc catgaccggt ggaacgcccc tgatccggca
     3301 agccattcgc agtgaattgc agtacttcgc caacgaactg gccactgacg tgcgtcatct
     3361 gggcctgctg ccgaacatgg agcagaccat gctggagaat gtggccctgc tgaccatcaa
     3421 tacggtggcg aatgccacca tggaactgct ggacctgccg gatggcaacg gtgctgcccg
     3481 tcagcagttc gaaaaacgcc tcaagcgcca cctgcaggtg attttcatcg gcgctgccca
     3541 ctggaagacc gcagcgccgg gctgatccgc ccggatttaa tcaggatcaa tatccccgcg
     3601 ctttgccccg ctttttagtc cacattgacg cgtccttttc tacacttaac aggaacggcc
     3661 accgctgagt cctgcgacag gaaaggaaat gccatgcgcg tcaacatgcc cgtcacccgg
     3721 aacgagatca agctcgctcc cggccagacc atcgtctcca aaaccacgcc caagggcgtc
     3781 atcacctacg tcaaccggga tttcgtcgag atcagcggtt tctccgaacc cgagctgatc
     3841 ggtcaggccc acaatctggt gcgccacccg gacatgccgc aagccgcatt caaggatcta
     3901 tgggacacgc tgcaggcggg caagccctgg cgtggcatgg tgaaaaaccg ctgcaagaac
     3961 ggcgactatt actgggtgga agccaacgtt acgccgatct ggtccggcgg acgggtgacg
     4021 gaatacatgt cggtacgcac cgcgccatcg caacagcaga tcgacgatgc ggaggcactt
     4081 tatgcgcgca tgaacgccgg tgagaaggtg cggccgtcgc tggcagccag gggcgagcgc
     4141 tatttccgca atctgccggt gaacgggaag ctgggctggc tggtgggggt gtttgcgctg
     4201 gtgttgctgg gggatttcct gctcagcggc gaagcgctgc cctggcctta cataatgaca
     4261 ttgctgattg cctgtggcgc gctgttccag tttgtgcagg acgtggtgaa aaagccgatg
     4321 caggaagcgg ccgttgcgct gcgcaaattc agcagcggtg atttcgacac ccatctgaat
     4381 attctgcgcg atgatgaacc gggtcaggtg ctgcagggca tgcagtgcat gaagatcaaa
     4441 ctcaatttcg aactggaaaa tacgcgcctg atcgccaacg aagcgctgcg ggtgaagaat
     4501 gcgctggatg tgtgcgacac caatgtcatg ctggcggacc cggattacaa catcatctac
     4561 atgaacgatt cactgcgcaa aatgatgaag aatgcggagg cggatctgaa aaccgtgctg
     4621 ccggcattcg atgcggaaaa gctgctgggc tgcaacatgg atatgttcca ccgcgaccgg
     4681 acgcatcagc gccgcattct ggataacgcg aaatccacgc accgccagga agtggtggtg
     4741 gcagggcgca ccattgtcat caccgccacg cccgtgttcg atcagggcgg taaacgcctg
     4801 ggcaccgtga ttgaatggct ggaccgcacc gaggaactgc gcaaggcagc cgagcagcag
     4861 cgcatcgaac agggtcggct ggatgcggag cgccgcgtgg ccaacgaaaa tgcgcgcatc
     4921 cggcaggcgc tggattccgt gtcggcgaat gtgatggtgg cggacaacga tctcaacgtc
     4981 gtctacacca atcacgcggt gatcagcatg atgcgcaagg cggaacgcga tatccagacc
     5041 gaactgccga atttccatgt cgacaaaatc atgggttcca atatcgatat ctatcacaag
     5101 aatccctcgc atcagcgcca gatgctgaag aatctaaaac agccggtgaa atcggtattc
     5161 ctgttgggcg gacgcacgct gcaggtgtat gccagcccgg taaccgacag tcacggtgaa
     5221 tccatcggca ccgtggtgga atggaccgac cgtaccgagg aagtggcgat cgaaaaagag
     5281 ctggatcaca tgatccggtc ggcggcgatt ggcaatctgt catcccgcgc cacagtgagc
     5341 ggacgcgccg gcttcttcct caccatgagt cagggcatca accagctgct ggaaatctgt
     5401 gaggacgtgg tgagcgaaat ggtcgccgtc atggagcggc tggcgcacgg cgatctgcgc
     5461 gccaagatca acaaggaata ccagggcgag ttcggccggc tgaaagacaa cgtgaatgaa
     5521 accgtggacc ggttggtgga tatcgttact gaaatccgca cggcgtcttc ggcggtgctg
     5581 aatggcgcag atgaaattgc ccagggcaat gcggatctga gccagcgcac ggaagagcag
     5641 gcgtcgtcgc tggaggaaac cgcgtcgagc atggaagaga tgaccagcgc ggtgcgccag
     5701 agtgcggaca atgcccggcg ggtagagcag ctcaccgccg caacgcggga gaatgcgcag
     5761 aagagtggtg aggtggcgca gcaggcagtg ggcgcgatca acgccatcaa tgtcagcagc
     5821 aaaaaaatcg ccgacatcat cggcgtgatc gacgagatcg ccttccagac caatctgctg
     5881 gcattgaacg ccgcggtgga agccgcgcgc gccggtgagc aggggcgcgg atttgccgtg
     5941 gtggcgggcg aagtgcgcag tctggcgcag cggtctgcca acgccgcgcg tgaaatcaag
     6001 gcgctgatta ccgaaagcgt gacccgggtg gaggatggca cggcgctggt gcgggagtcg
     6061 ggcaacatgc tcaaccagat catccagtcg gtgaacgatg tgagcgggct gatcagcgaa
     6121 ctggcgcagt cggcacagga gcaatcctcc ggtatcgatc aggtgaatac ggccgtggcg
     6181 cagatggacg aaatgacgca gcagaatgcg gcgctggtgg aggaggcatc tgccgccagc
     6241 gaggccatgg cagagcaggc gcgcaagctg ggcgagcaga ttgcgttttt ccggctggat
     6301 gataccgagc gcactccggc ccggcgcgct gcaccggttg cgtctggcgg tcgtgccgca
     6361 gcgccccggg cggtggcaca gcctccgcgg tcgcgcggtc atcagagcag tgatgagtgg
     6421 gaagaattct gaggcaggcg ggttgttgaa aaaacgattg catccgtagc gtttttcaac
     6481 agcggtatcg tctgtcgggg tgtccactgc aggtggtttc gcctgcaggt ttctctcagt
     6541 tctggttgtg aagggtaacc ctgttgcgac cggcttcctt cgacagatac agcgcgctgt
     6601 cggcgaattc cagcaccagt tcgggttttt gatggggctg gggtttgatc actgccaggc
     6661 caatgctcac ggtgacaggt acgcgctggt ccttcaccat gaagttcatc agctctacat
     6721 tgcggcgaat gcgttcagcc acggtgagcg cgccttcggt ttcggtttcc ggcaggatca
     6781 cgcagaattc ctcaccacca taccgggcgg cgcgatccga tggccagcgc agtcccttgc
     6841 gtacgctgtg tgccacgtct ttcagacatt cgtcgccgat caggtggccg taggtgtcgt
     6901 tgaagcgctt gaagtgatca atgtccatca gcagcaccga cagcgtgtga ccgtaacggg
     6961 cgcaacgggc aaattcctct gccagcgtat tatcgagata gcgacggttc ttcagtccgg
     7021 tcagctggtc ggtggc
//
LOCUS       DASUEG010000206         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_956167, whole genome shotgun sequence.
ACCESSION   DASUEG010000206 DASUEG010000000
VERSION     DASUEG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956167"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VFX11_03950"
     CDS             <1..1528
                     /locus_tag="VFX11_03950"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019411456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX4937802.1"
                     /translation="VTMIKMDPYINVDPGTMSPYQHGEVFVTNDGAETDLDLGHYERF
                     IRNTMTKKNNFTSGRIYLDVIEKERRGDYLGATIQVIPHITDEIKNRIVNSDLGVDVL
                     IVEIGGTVGDIESQPFLEAVRQLRVELGSNNSVFIHLTLVPYIDTAGETKTKPTQHSV
                     KELRSIGLQPDVLLCRSDHKIPDSSLDKIALFTNVERRAVIALQDVNTIYKIPSVLHA
                     QGLDDFVVEKLHLQCGPADLSEWTRVVDAQLNPQHEVTIAMVGKYTDLADAYKSLNEA
                     LLHAGIQTRTKVIIDYVDSEDIERDGVEKLAHYDGILVPGGFGKRGTEGNIAAIRYAR
                     EHGVPYLGICLGMQLAVIEYARNMAGLSDAHSSEFNKGARTPVVGLITEWIDEDGSVK
                     TRSENSDLGGTMRLGAQDCKLVKGSISRELYGKDVISERHRHRYEVNNKYVPELEKAG
                     LRVAGRSMDGSLVEMIEVPSHPWFVACQFHPEFTSTPRDGHPLFTGYVKAAKAHKDQK
                     "
     gene            1596..2465
                     /gene="kdsA"
                     /locus_tag="VFX11_03955"
     CDS             1596..2465
                     /gene="kdsA"
                     /locus_tag="VFX11_03955"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX4937803.1"
                     /translation="MSAQSQQVTAQKILELGKIRMGNELPFVLFGGMNVLESRDMAMQ
                     VAETYVKVTQKLGIPYVFKASFDKANRSSINSYRGPGLDEGLKIFEEIKKTFNVPIIT
                     DVHEPHQAAPVAEVCDIIQLPAFLSRQTDLVVAMARTGAIINVKKAQFLAPREMKHII
                     KKCEEAGNDKVILCERGSSFGYNNLVVDMLGFGIMKEFGAPVFFDVTHSLQMPGGLAE
                     AAGGRRAQVTELALAGMSQGLAGLFLEAHPDPDNAKCDGPCALRLNKLEPFLARVKAI
                     DSLVKSFEKLDTH"
     gene            2502..>3097
                     /gene="eno"
                     /locus_tag="VFX11_03960"
     CDS             2502..>3097
                     /gene="eno"
                     /locus_tag="VFX11_03960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013660353.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX4937804.1"
                     /translation="MSKIAQIRAREVLDSRGNPTIEADVILESGAAGTACAPSGASTG
                     SREALELRDGDKSRYLGKGVLKAVEFANKDIFKLLQGKDATQQRLIDKLMIDADGTEN
                     KSKFGANAILAVSLATAKAAAVHKVEPLFQHISEMIDGKAGPYTMPVPMMNIINGGAH
                     ADNNVDIQEFMIQPVGAPSFREALRWGAEIFHSLKAVL"
BASE COUNT          715 a          934 c          898 g          550 t
ORIGIN      
        1 ggtcacgatg atcaagatgg acccctacat caacgtcgac ccgggcacca tgagccccta
       61 ccagcatggc gaagtgttcg tcaccaatga cggcgccgaa accgacctgg accttggcca
      121 ttacgaacgc ttcattcgta ataccatgac caaaaagaac aacttcacgt ccggccgcat
      181 ctatctggac gtgatcgaga aggagcgccg gggcgattac ctgggcgcca ccatccaggt
      241 cattccccac atcaccgacg aaatcaagaa ccgcatcgtc aacagcgatc tgggcgttga
      301 tgtgctgatc gtggaaatcg gcggcacagt gggcgacatc gaatcgcagc cgtttctgga
      361 agccgtccgt cagttgcggg tggaactggg ttccaataac tccgtgttca ttcacctgac
      421 cctggtgccc tacatcgata cggcaggaga aaccaagacc aagcccaccc agcattcggt
      481 gaaggaactg cgttccatcg gcttgcagcc cgacgtattg ctatgccgtt ccgaccacaa
      541 aattccggat tcctccctcg acaagatcgc cctgttcacc aacgtggaac ggcgcgcggt
      601 cattgcgctg caggatgtga acaccatcta taaaatcccg tccgtgctgc atgcgcaggg
      661 gctggatgat ttcgtggtgg aaaaactgca cctgcagtgc ggcccggcgg acctgtccga
      721 atggacccgc gtggtggatg cgcagctcaa tccccagcac gaagtcacca tcgccatggt
      781 gggcaagtac accgatctgg cggatgccta caaatccctg aacgaagcgc tgctgcacgc
      841 cggcatccag acccgcacca aggtcatcat cgattacgtc gactccgaag acatcgagcg
      901 cgatggcgtg gaaaagctgg cccattacga cggcatcctg gtgcccggcg gtttcggcaa
      961 gcgcggcacc gaaggcaaca tcgccgcgat ccgctatgcc cgcgaacacg gcgttcccta
     1021 cctgggtatc tgcctgggca tgcagctggc ggtgatcgaa tatgcccgca acatggctgg
     1081 cctgtccgat gcccacagca gcgaattcaa caagggcgcc cgcaccccgg tggtggggct
     1141 gatcaccgag tggatcgatg aagacggctc ggtgaaaacc cgcagcgaaa actccgatct
     1201 gggcggcacc atgcgtctgg gcgcgcagga ctgcaaactg gtgaaaggca gcatctcccg
     1261 cgagctgtac ggcaaggatg tgatttccga acgtcaccgt caccgttacg aagtgaacaa
     1321 caaatacgtc ccggaactgg aaaaggccgg cctgcgcgtg gctggccgtt ccatggacgg
     1381 cagcctggtg gaaatgatcg aagtgccctc gcatccctgg tttgtcgcct gtcagttcca
     1441 cccggagttt acctccacgc cccgtgacgg ccatccgctg ttcaccggtt acgtgaaggc
     1501 ggcgaaagcg cacaaggacc agaaataaag ggaccagaag tgaagggatt caaaggtcga
     1561 ttgaaaggta ccgtgatccg caacaggaga ctgccatgag cgcacagtca caacaggtca
     1621 cagcgcaaaa gattctggag ctgggcaaga tccgcatggg caacgaactg cccttcgtgt
     1681 tgttcggcgg catgaacgtg ctggaatccc gcgacatggc catgcaggtg gcggaaacct
     1741 acgtgaaggt gacgcagaaa ctgggcattc cctatgtgtt caaggcgtcc ttcgacaagg
     1801 ccaaccgctc ctcgatcaac tcctatcgcg gccctggtct ggatgaaggt ctgaagatct
     1861 ttgaagaaat caaaaagacc ttcaacgtac ccattatcac tgacgtgcac gaaccccatc
     1921 aggccgcgcc ggtggcggaa gtgtgcgaca tcatccagtt gcccgcgttt ctgtcacggc
     1981 agacggatct ggtggtggcc atggccagaa ccggcgccat catcaatgtg aagaaagcgc
     2041 agtttctcgc cccgcgcgag atgaagcaca tcatcaagaa gtgcgaagag gcgggtaacg
     2101 acaaggtcat cctgtgtgag cgcggttcca gtttcggcta caacaatctg gtggtcgaca
     2161 tgctgggctt cggcatcatg aaggaattcg gcgcgccggt gtttttcgac gtgacccact
     2221 cgctgcagat gccgggtgga ctggcggaag cggcgggcgg tcgccgcgcg caggttaccg
     2281 aactggcact ggcgggcatg tcgcaaggat tggcgggcct gttcctggaa gcgcatcccg
     2341 atccggacaa cgccaagtgc gacggcccct gcgcgctgcg gctaaacaaa ctcgagccgt
     2401 tcctggcccg ggtgaaagcc atcgacagcc tggtaaaaag tttcgaaaaa ctggatacgc
     2461 actgaaacac tcaagcagtc accacaggag acatctttac aatgtccaag atcgcccaaa
     2521 tccgtgcccg tgaagtcctc gactcccgtg gcaatcccac catcgaagcg gacgtgattc
     2581 tcgaatccgg cgccgccggc acagcctgtg ctccgtcggg cgcgtccacc ggttcccgtg
     2641 aagcgctcga actgcgtgat ggcgacaaga gccgttacct gggcaagggt gttctgaaag
     2701 cggttgaatt cgccaacaag gacatcttca aactgctgca aggcaaggac gcgacccagc
     2761 agcgcctgat cgacaagctg atgatcgacg ccgacggcac cgagaacaaa tccaagttcg
     2821 gtgccaacgc gatcctggcg gtgtcgctgg ccacggcgaa agccgctgcc gtgcacaagg
     2881 ttgagccgct gttccagcac atctccgaaa tgatcgacgg caaggccggc ccctacacca
     2941 tgccggtgcc gatgatgaac atcatcaacg gcggcgccca tgccgacaac aacgtcgaca
     3001 ttcaggaatt catgatccag cccgttggcg cgccgagctt ccgcgaagcc ctgcgctggg
     3061 gcgcggagat tttccattcg ctgaaagcgg tgctgaa
//
LOCUS       DASUEG010000207         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_119855, whole genome shotgun sequence.
ACCESSION   DASUEG010000207 DASUEG010000000
VERSION     DASUEG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119855"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /gene="mutS"
                     /locus_tag="VFX11_03965"
     CDS             complement(<1..1618)
                     /gene="mutS"
                     /locus_tag="VFX11_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX4937805.1"
                     /translation="MSIVEEQYSQHTPMMQQYLRIKAQHPHELVFYRMGDFYELFYGD
                     AQKAADLLDITLTARGQSGGAPIPMAGVPYHAAENYLARLVKLGVSVAVAEQMGDPAL
                     AKGPVDRKVVRIVTPGTISDEALLDESQDNLLCAITQHGERYGIAHLDISSGRFFVCE
                     VEGEAALEAELGRINPAELLVAEDSHVLKKVQNRRGVRSRPVWEFDTESARRALIQQF
                     ETQDLKGFGCDHLPLAIATAGCLLQYAKETQRAALPHIRGLSLEAQDDCVILDAATRR
                     NLELTENLKGDDQHTLAWVLDKTKTAMGGRMLRRWINRPLRKRKLLLERQNAIAALLQ
                     NYQYEPVQAVLKQIGDIERILARIALKSARPRDLSRLRDALNLLPSLQSELAQFAEPV
                     IARLGERISLYPALAETLTKALVETPPVLMRDGGVIATGYDAELDELRALSENAGQFL
                     LDLEEREKARTGISTLKVGYNRIHGYYIEISRAQAKQAPEDYMRRQTLKNAERYITPE
                     LKAFEDKALSAKSRALAREKGLYDALLDIIS"
     gene            1803..>2103
                     /locus_tag="VFX11_03970"
     CDS             1803..>2103
                     /locus_tag="VFX11_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX4937806.1"
                     /translation="MLTYADQTLLIEALATQLGTLLRGRGLYVVTAESCTGGWVAEAI
                     TQIAGSSGWFDRGFVTYSNDAKREMLGVKRRTLERHGAVSEEVAREMALGALDRSP"
BASE COUNT          334 a          580 c          731 g          458 t
ORIGIN      
        1 ccgagatgat gtcgagcagg gcgtcgtaaa gtcctttctc acgggccagg gcgcggctct
       61 tggcgctgag ggccttgtct tcgaatgctt tcagttccgg cgtgatgtag cgttcggcgt
      121 ttttcagtgt ttgccggcgc atgtagtctt ccggtgcttg cttggcctgg gcgcggctga
      181 tttcgatgta gtagccgtgg atgcggttgt aaccgacttt cagcgtggag ataccggtgc
      241 gggctttttc gcgctcttcc agatctaata aaaattgccc ggcgttttcg cttagggcgc
      301 gcagttcgtc gagttcggca tcgtagccgg tggcgatgac gccgccgtcg cgcatgagga
      361 cgggtggggt ttccaccagg gctttggtga gggtttccgc cagagcagga tagagactga
      421 tgcgctcgcc gagccgggcg atgacgggtt ctgcaaattg cgccagttca ctctgcagcg
      481 atggcagcag gttcagcgca tcgcgcagac gggagaggtc gcgcgggcgg gcggatttaa
      541 gcgcgatgcg cgcgagaatg cgttcgatgt caccgatctg tttcaggact gcctggaccg
      601 gttcgtattg atagttttgc agcagggcag cgatggcgtt ctggcgttcc agcagcagct
      661 ttcttttacg caagggccgg ttgatccagc gccgcagcat gcggccgccc atggcggttt
      721 tggttttgtc gagcacccag gccagggtgt gctggtcgtc gcctttcagg ttttcggtca
      781 gctccaggtt gcggcgggtg gcagcgtcca ggatcacgca gtcgtcctgg gcttccaggc
      841 tgaggccgcg aatgtggggc agggcggcgc gctgggtttc cttggcgtat tgcagcaggc
      901 agccggcggt ggcgatggcc agcggcagat ggtcgcagcc gaaacctttc aggtcttgcg
      961 tttcgaattg ctggatcagg gcgcggcggg cggattcggt gtcgaattcc cagactggac
     1021 gcgatcgcac gccacggcgg ttctgcactt ttttcagaac gtggctgtct tcggccacca
     1081 gcagttcagc ggggttgatg cggcccagtt cggcctccag cgcggcttcg ccttccactt
     1141 cacagacgaa gaaacggccg ctgctgatgt cgaggtgggc gatgccgtaa cgctcgccat
     1201 gctgggtgat ggcgcagagc aggttgtcct gggattcgtc cagcagggct tcgtcgctga
     1261 tggtgccggg ggtgacgatg cgcactactt tgcggtccac agggcctttg gccagggcgg
     1321 gatcgcccat ttgttccgcc accgccactg acacgcccag cttcaccagc cgcgccaggt
     1381 aattttccgc cgcatggtag ggcacgcccg ccatggggat cggtgcaccg ccggactggc
     1441 cgcgggcggt gagggtgatg tcgagcaggt cggcagcctt ctgggcgtcg ccgtagaaca
     1501 gttcgtagaa atcccccatg cggtagaaca ccagttcgtg gggatgctgg gccttgatgc
     1561 gcaggtattg ctgcatcatg ggggtgtgct gggaatactg ttcttcgacg atggacatgg
     1621 cggcctgcga aatcgggaat ggctcggtca aaagccgtca ttgtacgcgt tcgcggcggc
     1681 tttggaaccg gttgacaggg gccggaaaaa tgccggatga cgccatggtc cgccctgggg
     1741 ggaacaggta caatcaggcc cattttctgc tcttcccttt catccagcca aaacggattt
     1801 cgatgcttac ctacgcagac cagaccctgt tgatcgaagc cctcgccacc caactgggta
     1861 cccttttgcg tggccggggt ctgtatgtgg tgacagcaga atcctgcacc ggtggctggg
     1921 tggcggaagc cattacccag attgccggca gctcgggctg gttcgaccgt ggtttcgtca
     1981 cctattccaa cgatgccaag cgcgagatgc tgggcgtgaa gcgccggacc ctggagcgcc
     2041 acggcgcggt gagcgaggag gtggcccggg agatggcgct gggggccctg gaccgaagtc
     2101 ccg
//
LOCUS       DASUEG010000208         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1454708, whole genome shotgun sequence.
ACCESSION   DASUEG010000208 DASUEG010000000
VERSION     DASUEG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454708"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1131)
                     /locus_tag="VFX11_03975"
     CDS             complement(118..1131)
                     /locus_tag="VFX11_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937807.1"
                     /translation="MSGFTDAGVMIRLAYQAMQEMGVDAFEVLRRIGLKHEQLAEKDL
                     RTPHSAQTLFWSVIEDITGDTDIGLHLGENIPVFKGQVIEYLFLSSATFGEGLKRALN
                     YQRLLSDAVNATLDQDAKGYFVGNITFTGGLRHLSECVVIGVLKFFDYVTEGRFKPLE
                     IHFEHAAGKHPEEYARVYKCPVLFSQPGNRIYFAPEIMNLPSGHAEPELLRLHEQLAS
                     EHVAKLEKQDFIAKVNRLIGELLESGNANLETVAERLGIKPRSLRTKLTEVDTNFNQL
                     LADYRCNLAKKLLARTDESIDEIVYLTGFSEPSTFYRAFKRWVGSTPVEYRKSKQKSL
                     IDE"
     gene            1425..>2256
                     /locus_tag="VFX11_03980"
     CDS             1425..>2256
                     /locus_tag="VFX11_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX4937808.1"
                     /translation="MSATIELDQAPSTLNLYLRAATAKKSGRLQGDALPKVKVCLKNV
                     TANPKKLATYRQVCGFPVSGSMPVTYPHILAFPLHMEILVNPLFPFPLLGLVHVRNEI
                     TQYRAIGNQEVLDIECELAGPHTVAKGLEFDIVTRVTAGGKVVWESVSTNLFRTKGNG
                     DKKDAEKKEDTFSADHIDFWNVPENIGRRYAKVSGDTNLIHIHAFTAKMFGFPRAIAH
                     GMWSKARALGALDSQLPKGPFRVSVAFKLPVFLPNKVQFLYTSTGDHTEFRIKDQQGV
                     K"
BASE COUNT          514 a          750 c          552 g          440 t
ORIGIN      
        1 gtcgttctcg tactggtccc cgagttcggc agttggtctt gtgtttcaag acacgccccg
       61 aaaccccacc cgctccactc tgcgactcgt ctggcacaaa gccaaatcca accaacctca
      121 ctcatcaatc aaactcttct gcttgctctt ccgatactcc accggcgtcg atcccaccca
      181 gcgtttgaac gcccggtaaa acgtactcgg ttccgaaaag cccgtcagat acacaatctc
      241 gtcaatcgac tcatccgtcc gcgccagcag cttcttcgcc agattgcagc gataatccgc
      301 cagcaactga ttgaaattgg tgtccacctc ggtgagcttg gtgcgcaaac tgcgcggctt
      361 gatccccaac cgctccgcca ccgtctccag attcgcattg cccgactcca gcaactcacc
      421 tatcagccgg ttcaccttgg caatgaaatc ctgcttctcc agcttcgcca cgtgctcact
      481 ggccagctgc tcgtgcaaac gcagcagttc cggctcggca tgaccggacg gcagattcat
      541 gatctccggc gcgaaataaa tccggttgcc cggctggctg aacagcaccg ggcatttgta
      601 cacccgcgca tattcctccg gatgcttgcc cgccgcgtgt tcgaaatgaa tctccagcgg
      661 tttgaaacga ccctcggtga cataatcgaa aaacttcagc acaccgatca ccacgcactc
      721 actcagatgc cgcagaccgc cggtgaacgt gatattgcct acgaaatagc ccttcgcatc
      781 ctgatccagc gtcgcattca ccgcatcgct caacagccgc tggtaattca gcgcccgctt
      841 caaaccctca ccaaaggttg cgctgctcag aaacagatat tcaatcacct gccccttgaa
      901 taccgggatg ttctccccca gatgaagacc tatgtcagta tcgccggtga tatcctcgat
      961 cacactccag aacagcgttt gcgcagaatg gggtgtgcgc agatcttttt cggccagctg
     1021 ctcgtgcttc agaccaatgc ggcgcaacac ttcaaaggca tcgaccccca tttcctgcat
     1081 ggcctggtag gcgagccgta tcatcacacc cgcatcggta aatccgctca ttcgtacctg
     1141 ctgtagggtc aattgcccgg gggaaaatag agaaccagaa acagtccaat aactgcatga
     1201 aataccggaa atttccggca agactgcggt aatgatagac agcacggcca ccattgacaa
     1261 tagagacgca aacaggctcc agccagcctg ccattgactg caccgactgt ccggaacggg
     1321 cccagggcaa ttgcggagac ccttgattcc tttatacttt ggcccacttt gcggcaccgg
     1381 cccaaccgcc gcgccaacgc atccaaccgt ccgaggaagg cgacatgtca gcaaccatcg
     1441 aactcgacca ggcaccctcc accctcaatc tgtacctgcg ggccgccacc gccaagaaat
     1501 ccggccgcct ccagggcgat gccctgccca aggtcaaggt ctgcctgaag aacgtgaccg
     1561 ccaacccgaa aaagctggcc acttaccgtc aggtgtgcgg ctttcccgtc agtggcagca
     1621 tgccggtcac ctatccgcac atcctggcgt ttccgctgca tatggagatt ctggtgaacc
     1681 ccctgttccc gttcccgctg ctgggtctgg tgcatgtgcg caatgagatt acccagtatc
     1741 gcgccatcgg caatcaggaa gtgctcgaca tcgaatgtga actggcgggc ccgcacaccg
     1801 tggccaaagg ccttgaattc gacatcgtca cccgagtgac cgccggcggc aaagtggtgt
     1861 gggaaagcgt gagcaccaac ctgttccgca ccaaaggcaa cggcgacaag aaagatgccg
     1921 agaagaagga agacacgttc tccgccgacc atatcgattt ctggaacgtg ccggagaaca
     1981 tcggccgccg ttatgccaaa gtgtccggcg acaccaacct gatccacatt cacgccttca
     2041 ccgcgaaaat gtttggcttc ccccgcgcca tcgcccacgg catgtggagc aaggcccgcg
     2101 cgctgggtgc gctggattcg caactgccga aaggcccgtt cagggtgagc gtggcgttca
     2161 agctgccggt gttcctgccc aacaaggtgc agttcctcta taccagcact ggcgaccaca
     2221 ccgagttccg catcaaggac cagcaaggcg tcaagc
//
LOCUS       DASUEG010000209         4598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_13511, whole genome shotgun sequence.
ACCESSION   DASUEG010000209 DASUEG010000000
VERSION     DASUEG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFX11_03985"
     CDS             <1..254
                     /locus_tag="VFX11_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX4937809.1"
                     /translation="FALIHLLRAIEIDFHDVLAKKNALVSMQQIIRELEASQQPVMSP
                     MILNGSGFGMLANHSLVMANYISRANAAIIDLRRLLEQG"
     gene            491..1123
                     /locus_tag="VFX11_03990"
     CDS             491..1123
                     /locus_tag="VFX11_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4937810.1"
                     /translation="MGLKQIVTGLGIGLMASAAQAGVIYNLSGAGVYSTQVAGATTVN
                     FNDGSCGAYVACYGNGALVTGSVSGKYASPYGITDRYLSVPYHSSSGSVTLQLDSTYD
                     YYGLYWGSVDTYNHVSFYLGGNLVDTFSGGDLAPLMAIGGQGSWTSNRFVNFFFDGGD
                     LFDRIVLTSTNYAFETDNHAFGKVAVPEPGTLALFALGLLGLGLSRRFTN"
     gene            complement(1182..1394)
                     /locus_tag="VFX11_03995"
     CDS             complement(1182..1394)
                     /locus_tag="VFX11_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937811.1"
                     /translation="MLVRISSGADDKFALAWCKTLLFRLNLMNYVLTWGGVNKKHATK
                     VVGKLRIWRGAIEYLRGEERQQDHRP"
     gene            1428..3617
                     /locus_tag="VFX11_04000"
     CDS             1428..3617
                     /locus_tag="VFX11_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006031795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX4937812.1"
                     /translation="MLFGRQDRKKIKILDKQVTDWKYTTCGYCSTGCSIEVGLDAQGR
                     AVATRGVANADVNRGKLCIKGIFEHELFDASGRGSVPIVRDKPWNPWQPTNWDSALDK
                     MAGEIKRIQSQYGRDSFAIVSTGQIMTEEFYTLGKLVRGCIGTNNYDGNTTLCMASAV
                     SGYTRSFGSDGPPGCYDDFEHTECMIAWGSNLPEQHPVIYWRLKEALEKRKFPLIVVD
                     PRVTMFAQFADIHLPITPGTDVVLQNALMHVILKEGLEDREYNNAHTKGIEALEACVR
                     DYDPTTAARICGIDEDTIRHVARLYAKAKCAMSIWTMGINQSTHGSDGVVGINNLNLI
                     TGNIGKPGGTSLSITGQCNAMGTREWSSCSGLPGYRKLEKPKDRKEIAEFWGVEESFF
                     PEKRGLFMTDIFPAIETGQIKGLWLIATNPMTSMPNTPRIRKTLEKLEFLVVQDAYQD
                     VEANQYAHMFLPAAVWAEKTGCFTNTERRVNLIRPVVPPRAESKPDFWIFTQLAKRFP
                     NGQKIRFPETPEGAFEEMKQLSKGAKRTLDISGMSHDLIEKSRGIQWPYREGMEGGKG
                     DPRLYVDGKFQTDDGRANLIPLPFIDNNEKPCTDFPFWLNSGRVVEHFHTRTRTGKIG
                     NCNKFSPTPYMEMNPDSAAEIGVQHQTYVRLVSRRGDAVVLVQLTQRVPRNMVFIPFH
                     FHDCVNRLSLGLLDPHSRQPAFKQCSVRIEHTDQALAAKLNAERRTF"
     gene            3630..>4598
                     /locus_tag="VFX11_04005"
     CDS             3630..>4598
                     /locus_tag="VFX11_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4937813.1"
                     /translation="MLNVLKSVRDKLDQIGAEPAPLFYEAKRIFEVRPEEQPYALLND
                     CESKRQNRYGVKIDLLDLSGGALPAGRSMYINENPAVGENPNRYKQHGFHFTADNCIG
                     CHACEAACSEKNGNPAHISFRSVGYVEGGTFPDYKRMNISMACNHCDDPVCLKGCPTR
                     AYTKHAEYGAVLQDPDTCFGCGYCTWVCPYNAPQLDPIQGQVSKCNMCVDRLEEGLKP
                     ACVSACLGNALNFGVIENTPENREQVLTQIPGFPDPSITQPNIRFQQTKTMPNVVTRT
                     DSMPVKYHKGEDGQFRSVVDQKQGKTISWNFKRLSSRENPLVIFTLA"
BASE COUNT          998 a         1323 c         1358 g          919 t
ORIGIN      
        1 cgttcgcgct gatccacctg ctgcgggcca tcgagatcga tttccacgac gtgctggcga
       61 agaagaacgc gctggtgagc atgcagcaga tcatccgtga gctggaagca tcgcagcagc
      121 cggtgatgag cccgatgatc ctgaacggct ccggcttcgg catgctggcc aaccactccc
      181 tggtgatggc gaactacatt tcccgcgcca acgccgccat catcgatctg cgccggttgc
      241 tggaacaggg ctgatccggc gtcctgccaa cgggtcgggc cgctggggtg gcctgacctg
      301 ctttacccct cgcgccgtgt gtcgcggccc aagtgtcgaa cggttttcca cttctgaagc
      361 cccctcatct gcgcggattc tctatcccgc tgaagattgt acaaatttta atcatggcac
      421 tctttctgct tatattctga tcaatagatg gtcgaatcgg ataacaacaa cgttgttagg
      481 ggagtgtttc atgggtctta agcagatagt gacaggatta ggcatcggac tgatggcatc
      541 ggctgcgcag gcgggtgtga tctacaacct gtccggcgca ggcgtctaca gtacccaggt
      601 agccggtgcc accaccgtga atttcaacga tggttcctgt ggtgcctatg ttgcttgcta
      661 tggcaacggg gcgctggtca cgggcagcgt ctctggcaag tacgcctcgc cttacggcat
      721 caccgaccgc tacctgagcg tgccctacca ttccagcagc ggcagcgtga ccctgcagct
      781 ggacagcacc tatgattact acggcctgta ctggggttcg gtggatacct acaaccatgt
      841 gtccttctat ctgggcggca atctggtgga cactttcagt ggtggcgatc tggctccgct
      901 gatggcgatc ggtggccagg gttcctggac atcgaaccgg tttgtgaatt tcttcttcga
      961 tggcggtgac ctgttcgacc gcatcgtgct gaccagcacc aactacgctt ttgaaacgga
     1021 caaccatgcg ttcggaaaag tggcggtgcc ggagccgggt acgctggcgc tgttcgcgct
     1081 gggtctgctg ggactgggtc tgagccgtcg cttcaccaac tgattttcca tcgcaggcgt
     1141 ccgatcagaa aaacccgctg ccaatgccgc agcgggtttt tttatgggcg atgatcctgc
     1201 tgccgttcct ccccgcgcag atattcgatt gcgccgcgcc aaatccttaa ttttcctacc
     1261 actttggtgg catgcttctt gttaactcct ccccaggtca acacgtaatt catcaaattc
     1321 aggcgaaaaa gcagtgtttt gcaccatgca agtgcgaatt tgtcatcggc acccgaactg
     1381 atgcgcacca gcatgacgcc tgaagagggg agagaggaac caaaacgatg ctttttggcc
     1441 gtcaggatcg caagaagatc aagatactcg acaaacaggt gacagactgg aagtacacca
     1501 cctgtggcta ctgttcgacg ggttgttcca ttgaagtggg gctggatgcg cagggccgtg
     1561 ccgtggcgac gcggggcgtg gcgaacgccg atgtgaatcg gggcaagctg tgcatcaagg
     1621 ggattttcga gcacgagctg tttgacgcgt ccgggcgcgg cagtgtgccg atcgtgcgcg
     1681 acaagccgtg gaatccctgg cagccgacca actgggacag cgcgctggac aagatggcgg
     1741 gcgaaatcaa gcgcatccag agccagtacg gccgcgattc ttttgcgatc gtgtccaccg
     1801 gccagatcat gacggaagag ttttacacgc tgggcaaact ggtacgtggc tgcatcggca
     1861 ccaacaatta cgatggcaat accacgctgt gcatggcgtc ggcggtgagt ggctatacgc
     1921 gctcgttcgg ctctgacggc ccgcccggtt gctacgacga tttcgagcac accgagtgca
     1981 tgatcgcgtg gggttccaat ctgccggaac agcatccggt gatttactgg cgcctgaagg
     2041 aagcgctgga aaaacgcaaa ttcccactga tcgtggtgga cccccgcgtc accatgttcg
     2101 cgcagttcgc cgatattcat ctgccgatca cgccgggcac cgatgtggta ctgcagaacg
     2161 cgctgatgca cgtgatcctg aaggaaggcc tggaagaccg cgagtacaac aacgcgcaca
     2221 ccaagggcat cgaggcgctg gaagcctgcg tcagggatta cgatcccacc accgccgcgc
     2281 gcatctgcgg catcgatgaa gacaccatcc gccacgtggc acgcctgtat gccaaggcca
     2341 aatgcgccat gagcatctgg accatgggta tcaaccagag cactcacggt tctgacggtg
     2401 tggtgggcat caataacctg aacctgatca ccggcaacat cggcaaaccc gggggcacca
     2461 gtctgtcgat cactggccag tgcaatgcga tgggcacgcg ggagtggtcc agctgctcgg
     2521 gcctgccggg ttaccgcaag ctggaaaagc cgaaggaccg caaggaaatc gctgaattct
     2581 ggggtgtgga ggaatccttc ttcccggaaa aacgtggcct gttcatgacc gacatttttc
     2641 ctgccatcga gactggccag atcaagggcc tgtggctgat cgccaccaat ccgatgacgt
     2701 cgatgcccaa tacgccgcgc atccgcaaga cgctggagaa gctggaattc ctggtggtgc
     2761 aggatgcgta tcaggacgtg gaagccaacc agtacgcgca catgtttttg cccgccgccg
     2821 tatgggcgga aaaaaccggt tgtttcacca ataccgaacg ccgcgtgaat ctgatccgcc
     2881 cggtggtgcc accgcgtgcg gaatcgaaac cggatttctg gatattcacg cagctggcca
     2941 agcgttttcc caatggccag aaaatcagat tcccggaaac accggaaggt gcgttcgagg
     3001 aaatgaagca gctgtcgaaa ggtgcgaagc gcacgctgga tatttccggt atgagccacg
     3061 acctgatcga aaaatcccgc ggcatccagt ggccataccg ggaaggcatg gaaggcggca
     3121 agggagatcc gcgcctgtat gtggacggca aattccagac ggacgatggc cgcgccaacc
     3181 tgattccatt gccattcatc gacaacaacg aaaagccctg cacggatttt ccgttctggc
     3241 tcaacagcgg ccgcgtggtg gagcatttcc acacccgcac ccgcaccggg aaaatcggca
     3301 actgcaacaa attcagcccg acgccctaca tggaaatgaa tccggattcc gccgccgaaa
     3361 tcggcgtgca gcatcagacc tatgtgcggc tggtgtcgcg ccggggcgat gcggtggtac
     3421 tggtgcaact gacgcagcgg gtgccgcgca acatggtgtt catcccgttc cactttcacg
     3481 attgtgtgaa ccggctgtcg ctgggactgc tcgatccgca ttcgcgccag cctgcgttca
     3541 aacagtgctc ggtgcgcatc gagcacacgg atcaggccct tgcggccaaa ctgaatgccg
     3601 aacggcgcac gttttaacgg agacaaatta tgttgaacgt actgaagtcg gtgcgcgaca
     3661 agctggacca gatcggcgcc gaaccggcac cgctgtttta cgaagccaaa cgcatttttg
     3721 aagtgcggcc ggaagagcag ccctatgcgc tgctgaacga ctgcgaatcg aagcggcaga
     3781 accgctacgg cgtgaaaatc gatttgctgg atctgagcgg tggcgcgttg ccggcaggcc
     3841 gctccatgta catcaatgaa aatccggcgg tgggcgagaa tccgaatcgc tacaagcagc
     3901 atggttttca cttcaccgcc gacaactgca ttgggtgtca tgcctgcgaa gcggcctgca
     3961 gcgagaagaa cggcaatccc gcgcacatca gcttccgcag tgtgggttac gtggaagggg
     4021 gcaccttccc cgactacaaa cgcatgaata tttccatggc ctgcaaccac tgcgacgatc
     4081 cggtgtgtct gaaaggctgc cccacccgtg cctataccaa gcacgcggaa tacggcgcag
     4141 tgctgcagga cccggacacc tgtttcggtt gcggttactg cacctgggtg tgcccgtaca
     4201 acgcgccgca gctggacccg atccagggtc aggtgtccaa gtgcaacatg tgcgtggacc
     4261 gtctggaaga agggttgaag cctgcatgcg tgtcggcgtg tctgggtaac gcactgaact
     4321 ttggcgtgat tgagaacaca ccggaaaatc gcgagcaggt gctgacccag attcccggtt
     4381 tccccgatcc gtccattacc cagcccaata tccgtttcca gcaaaccaaa accatgccca
     4441 acgtggtgac ccgcaccgac tccatgccgg tgaaatacca caagggtgaa gacggccagt
     4501 tccggtcggt ggtggaccag aagcagggca aaaccatcag ctggaatttc aaacgcctga
     4561 gttcgcgcga gaatccgctg gtgattttca cgctggcg
//
LOCUS       DASUEG010000210         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1527299, whole genome shotgun sequence.
ACCESSION   DASUEG010000210 DASUEG010000000
VERSION     DASUEG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527299"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /locus_tag="VFX11_04010"
     CDS             <1..2323
                     /locus_tag="VFX11_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4937814.1"
                     /translation="LGGASYSRAAFLNGQPAAPLAIKLSPSGNAVETAALVRARMAEL
                     APYFPKGMIWDIPYDTSAFVSISIQEVVKTLLEAIALVFLVMLVFLQNWRATLIPTLV
                     VPVALMGTFIGMNLFGFSINVLTLFGLVLAIGILVDDAIVVVENVERIMTEEGLSPLA
                     ATRKAMSQITGAIIGITTVLVAVFIPMAFFGGSVGAIYRQFSLALIVSILFSAFLALS
                     LTPALCATLLKRHPHDGPRKGFFGWFNRAFAATTRGYETGVRHILKRSLRFFVLYMVV
                     VTAVTWFAVRLPTAFIPEEDQGYFITIVQLPAGATQQRTLAVLRQMENYFLKNEPNVA
                     KMVAVAGFSFFGGGQNVGIAFIRMKDWDERPNPEQHVTAIVGRARAYLSTLGDANIFP
                     LNPPSIPELGTATGFEFQLQDRGGQGHVALTEARNQLLSLASKHPALASVRGEGLEDT
                     PQLDVQIDRVKASALGVAISDINGTLSIALGSAYANDFVRAGRIQKVLVQADAPARML
                     PEQVLNLRVRNNQGHMVPFSAFAHARWIMGSPRLERYNGMPSMKIGGQAAPGFSSGEA
                     MRAMEQLAAKLPDGFGYEWSGTSYEERLSGAQAPGLFVISVLVVFLCLAALYESWTIP
                     VSVLLVVPLGILGSLAAVYLRDMPNDVYFKVGLITIIGLAAKNAILIVEFAKDQMALG
                     KPLLDATLDACRMRFRPIIMTSLAFAFGVLPLAISTGAGAASRHAIGTGVLGGMISAT
                     VLAVLFVPLFFVMVIKYFPKGKEREGEWRGDRS"
BASE COUNT          405 a          733 c          776 g          495 t
ORIGIN      
        1 tctgggcggc gccagctatt cccgcgccgc gttcctcaat ggccagcccg ccgcgccgct
       61 ggcgatcaag ctgtcgccgt ccggcaacgc ggtggaaacc gccgccctgg tgcgcgcgcg
      121 catggcggaa ctggcaccgt attttcccaa gggcatgatc tgggacattc cctacgacac
      181 ctccgccttc gtcagcattt ccatccagga agtcgtgaag acgctgctgg aagccatcgc
      241 gctggtgttc ctggtgatgc tggtattcct gcagaactgg cgcgcaacac tgatccccac
      301 cctggtggtg ccggtggcgc tgatgggcac cttcatcggc atgaacctgt ttggtttttc
      361 catcaacgtg ctgacgctgt tcgggctggt gctggccatc ggtattctgg tggacgacgc
      421 catcgtggtg gtggagaacg tcgagcgcat catgacggaa gaagggctgt cgccgctggc
      481 ggccacccgc aaggccatga gccagatcac cggcgcgatc atcggcatca ccacggtgtt
      541 ggtggcggtg ttcattccga tggcattttt cggcggctcg gtgggcgcga tttaccggca
      601 attctcgctg gcgctgattg tgtcgatcct gttctcggcc tttctggccc tgagcctgac
      661 accggcactc tgtgccaccc tgctcaagcg ccacccgcac gacggcccgc gcaagggttt
      721 cttcggctgg ttcaaccgcg ccttcgccgc caccacccgt ggctatgaaa ccggcgtgcg
      781 ccatatcctg aaacgcagcc tgcgcttttt cgtgctgtat atggtggtag tgaccgcggt
      841 gacctggttc gctgtgcgcc tgcccaccgc gttcattccg gaagaagacc agggctactt
      901 catcaccatc gtgcaactgc cggccggtgc cacccagcag cgcacgctgg cggtgctgcg
      961 gcagatggaa aattattttc tcaagaacga acccaacgtt gccaagatgg tggcggtggc
     1021 aggtttcagc tttttcggcg gcggccagaa cgtgggcatc gctttcatcc ggatgaaaga
     1081 ctgggatgaa cggcccaatc ccgagcagca tgtgacggcc atcgtgggac gcgcccgcgc
     1141 ctatttgtcc accctggggg acgccaacat tttccccctc aatccgccgt cgattcccga
     1201 actgggcacg gccaccggct ttgaattcca gctgcaggat cgcggcggcc agggccatgt
     1261 ggcgctgaca gaggcgcgca accagctgct gagcctggcg tccaagcatc cggcactggc
     1321 aagcgtgcgg ggagaaggac tggaagacac gccgcaactg gacgtgcaga tcgaccgcgt
     1381 caaggccagt gcactgggcg tggccatcag cgacatcaac ggcaccctgt cgatcgcgct
     1441 gggctcggct tacgccaacg atttcgtgcg cgccggccgc atccagaaag tgctggtgca
     1501 ggccgatgca ccggcgcgca tgctgccgga acaggtgctg aacctgcggg tgcgcaacaa
     1561 ccagggccac atggtgccct tcagtgcctt cgcccacgcc cgctggatca tgggatcgcc
     1621 gcgactggaa cgttacaacg gcatgccgtc gatgaaaatt ggcggccagg cggcgcccgg
     1681 tttcagttcc ggtgaagcga tgcgagcgat ggaacagctg gccgccaaac tgccggatgg
     1741 tttcggctac gaatggtcgg gcacgtcgta tgaagagcgg ctgtcgggtg cgcaggcgcc
     1801 ggggctgttc gtgatttcgg tgctggtggt gttcctgtgt ctggcggcgc tgtacgaaag
     1861 ctggaccatt ccggtatcgg tgctgctggt ggtgccgctg ggcatattgg gatcgctggc
     1921 ggcggtgtat ctgcgcgaca tgccgaacga tgtttatttc aaggtgggcc tgatcaccat
     1981 catcggtctg gcggcgaaaa atgcgatcct gatcgtggaa ttcgccaagg atcagatggc
     2041 gctcggcaaa ccgctgctgg atgccacgct ggacgcctgc cgcatgcggt tccgcccgat
     2101 catcatgacg tcactggcat ttgcgttcgg cgtgctgccg ctggcgatca gcactggtgc
     2161 cggcgcggcc agccggcatg ccatcggcac cggtgtgctg ggcgggatga tctcggccac
     2221 cgtgctggca gtgttgtttg tgccgctgtt ttttgtgatg gtgataaagt attttccgaa
     2281 ggggaaggaa agggaggggg agtggcgcgg cgatcgctcc tgatctgttg accctgcata
     2341 gtcgacgatt tctataccgt tggtgatctc ccgcaatcac ccccgacgcg tatcactgcg
     2401 cctccacct
//
LOCUS       DASUEG010000211         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2172661, whole genome shotgun sequence.
ACCESSION   DASUEG010000211 DASUEG010000000
VERSION     DASUEG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172661"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2437
                     /gene="rpoB"
                     /locus_tag="VFX11_04015"
     CDS             <1..2437
                     /gene="rpoB"
                     /locus_tag="VFX11_04015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX4937815.1"
                     /translation="GFEVRDVHPTHYGRVCPIETPEGPNIGLINSLATFARANDYGFL
                     ESPYRRVQNGKVLDDVIYLSAIEEGEYVIAQANAEVDEDGMLVEDLVPVRHMNEFTVT
                     TADNVTLMDVSPRQVVSVAASLIPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTG
                     MEGIVARDSGVCQVARRGGLVDRVDAARIVVRVHDSEVGTTDDAGVDIYNLTKYTRSN
                     QNTCINQRPLVKVGDKVARGDILADGPSVDMGELALGQNMRVAFMPWNGYNFEDSIML
                     SERVVQEDRFTTIHIQELTCVARDTKLGPEEITADIPNVGEAALSKLDESGIVHIGAE
                     VVAGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGTKGTVIDVQVF
                     TRDGVQKDQRAIDIENAEIDKFRKDLNDEFRIIQGAALQRLRRTLNGEKVSAAGGGLK
                     KGDTITDDILGSLEPAQWFDLRPSSDQVAEQLESIKSYLEEVRADQEKKLAEKKRKIS
                     SGDDLAHGVLKIVKVYLAVKRRVQPGDKMAGRHGNKGVISVIMPVEDMPYDADGVPVD
                     VVLNPLGVPSRMNVGQILETHLGLAAHGLGHRIGEMLKQKQDAAKLREFLEKVYNGIG
                     EKAEDLASFTDGEIFELAQNLKRGVPMATPVFDGAKESEIKEMLVLGGQPENGQMVLF
                     DGRTGERFDCPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFG
                     EMEVWALEAYGAAYTLQEMLTVKSDDVNGRTRIYKNIVDGDHRMDPGMPESFNVLVKE
                     IRSLGIDIELENE"
     gene            2503..>3612
                     /locus_tag="VFX11_04020"
     CDS             2503..>3612
                     /locus_tag="VFX11_04020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817147.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4937816.1"
                     /translation="MKDLLNLLKAQGQDAEFDRIRIGLASPEMIRAWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRDRM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFESFVVTEPGTTSLERGQML
                     TDEQYYEALEEFGDEFEASMGAEAIQRLLQDMDLEQEIRELREELPTINSETKVKKVT
                     KRLKLMEAFHESGNDPQWMILSVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGQRVDYSGRSVIVVGPTLRLHQCGLPK"
BASE COUNT          794 a         1031 c         1111 g          676 t
ORIGIN      
        1 cggtttcgaa gtgcgcgacg tgcatcccac ccattacggc cgggtctgcc cgatcgaaac
       61 gccggaaggt ccgaacatcg gtctgatcaa ctcgctcgca acctttgccc gcgccaacga
      121 ttacggcttc ctggaaagcc cttatcgtcg cgtgcagaac ggcaaggtgc tggatgacgt
      181 gatttacctg tcggcgatcg aggaaggcga gtacgtgatc gcgcaggcga acgcggaagt
      241 cgatgaagac ggcatgctgg tggaagatct ggtgccagta cgtcacatga acgaattcac
      301 tgtgaccacc gccgacaacg ttaccctgat ggacgtatcg ccccgtcagg tcgtatcggt
      361 tgctgcgtcg ctgattccgt tcctggagca tgatgacgcg aaccgcgcac tgatgggctc
      421 gaacatgcag cgtcaagcgg ttcccactct gcgttccgag aagccgctgg tcggtaccgg
      481 catggaaggc atcgtggcgc gtgactccgg tgtgtgccag gttgcccgcc gtggcggctt
      541 ggttgaccgc gttgatgctg cgcgtatcgt ggtgcgcgtg catgacagcg aagtcggtac
      601 taccgatgac gctggtgtcg acatctacaa cctgaccaaa tacacccgct ccaaccagaa
      661 cacctgtatc aaccagcgtc cgctggtgaa agtgggtgac aaggtggcgc ggggagacat
      721 cctggccgac ggtccgtccg tggacatggg tgagctggcc ctgggccaga acatgcgcgt
      781 cgcgttcatg ccctggaacg gttacaactt cgaagactcg atcatgctgt ccgagcgtgt
      841 ggtgcaggaa gatcgcttca ccacgatcca cattcaggaa ctgacctgtg tggcccgtga
      901 caccaagctg ggtcccgagg aaatcaccgc ggatattccg aacgtgggtg aggccgccct
      961 gtccaaactg gacgagtccg gcatcgtgca cattggcgcc gaagtggtgg caggtgacat
     1021 cctggtaggc aaggtcacgc cgaaaggcga aacccagctg accccggaag aaaaactgct
     1081 gcgcgccatt ttcggtgaga aagcatctga cgtgaaggac acctctctgc gcgtgccgtc
     1141 cggcaccaag ggtactgtca tcgacgtgca ggtgttcacc cgtgatggcg tgcagaaaga
     1201 ccagcgcgcc atcgacatcg agaatgccga gatcgacaag ttccgcaagg acctgaatga
     1261 cgagttccgc atcatccagg gcgccgcgct gcagcgtctg cgtcgtaccc tgaatggcga
     1321 aaaagtgtcg gcagccggtg gtggcctgaa gaaaggcgac accatcaccg acgacatcct
     1381 gggttcgctg gaaccggcgc agtggttcga tctgcgtccg tcctcagatc aggttgcgga
     1441 gcagctggaa agcatcaagt cctacctgga ggaagtgcgc gccgatcagg aaaagaaact
     1501 ggcggagaag aagcgcaaga tttccagtgg cgacgatctg gcccatggcg tgctgaaaat
     1561 cgtcaaggtc tatctggccg tgaaacgtcg cgtacagccc ggtgacaaga tggcgggccg
     1621 ccacggtaac aagggtgtta tctcggtcat catgccagtt gaagacatgc cttatgacgc
     1681 cgatggcgtg ccggttgatg tggtgctgaa cccgctgggc gtaccttcgc gtatgaacgt
     1741 cgggcagatc ctggaaacgc atctggggct ggcggcgcac ggtctgggtc atcgcattgg
     1801 cgaaatgctc aagcagaagc aggatgctgc caagctgcgc gagtttctgg aaaaggtcta
     1861 caacggcatc ggcgagaaag ccgaggatct ggccagcttc actgacggcg agattttcga
     1921 gctggcgcag aacctgaagc gcggcgttcc catggcaacc ccggtatttg acggcgccaa
     1981 ggaatccgag atcaaggaaa tgctggtgct gggcggtcaa cccgaaaacg gccagatggt
     2041 tctgttcgat ggccgcaccg gcgagcgttt cgactgtccc gtaaccgtgg gttacatgta
     2101 catgctgaag ctgaaccacc tggtggacga caagatgcat gcgcgttcca ccggttcgta
     2161 cagcctggtt acccagcagc cgctgggtgg taaggcgcag ttcggcggcc agcgtttcgg
     2221 agagatggaa gtgtgggcac tggaagcata cggtgccgcc tacaccttgc aggaaatgct
     2281 gacagtgaaa tcggacgacg tgaacggacg tacccgcatc tacaagaaca tcgtggatgg
     2341 cgaccaccgt atggatcccg gcatgccgga atccttcaac gtactggtca aggaaatccg
     2401 ttccctcggt atcgatatcg agctggaaaa cgaataacgg ttgatacaaa tacggcggac
     2461 aggattctgt ccgcctaact ggctcccatt ggaggatctt cagtgaaaga cctactgaac
     2521 ctgctgaaag cacagggaca agacgcggaa ttcgatcgca ttcgcattgg cctggcgtca
     2581 ccggaaatga ttcgcgcctg gtcgtttggc gaagtcaaaa aacctgaaac catcaactac
     2641 cgtacgttca aacccgagcg tgacggcctg ttctgcgccc gcatctttgg tcccatcaag
     2701 gactatgagt gcctgtgcgg aaagtacaag cgcctgaagc accgcggcgt gatctgcgag
     2761 aagtgcggcg tggaagtcac gctgtcgaaa gtgcgtcgcg atcgcatggg ccatatcgaa
     2821 ctggccagcc ccgtcgcgca catctggttc ctgaagtcgc tgccgtcccg tatcggcctg
     2881 ctgctggaca tgaccctgcg tgacatcgag cgcgtgctgt atttcgaaag cttcgtcgtg
     2941 accgaaccgg gcaccacctc gctggaacgc ggccagatgc tgaccgacga acagtattac
     3001 gaagccctgg aagaattcgg tgacgaattc gaagccagca tgggtgccga agcgattcag
     3061 cgcctgctgc aggacatgga tctggagcag gaaatccgcg aactgcggga ggagctgcct
     3121 accatcaact ccgagaccaa ggtgaagaaa gtcaccaagc gtctgaagct gatggaagcg
     3181 ttccacgaat ccggcaacga tccccagtgg atgatcctga gcgtactgcc cgtgctacca
     3241 ccggacctgc gtccgctggt accgctggat ggcggccgtt ttgcgacctc cgatctgaac
     3301 gatctgtacc gtcgcgtcat caaccgtaac aaccgtctga agcgtcttct cgacctgaac
     3361 gcgccggaca ttatcgtgcg caacgagaaa cgcatgctgc aggaagcggt tgacgcgctg
     3421 ctggataacg gccgccgtgg ccgcgccatc accggttcca acaagcgccc gctgaaatcc
     3481 ctggctgaca tgatcaaggg caagcagggt cgtttccgcc agaacctgct gggccagcgc
     3541 gtcgactact ccggccgttc cgtgatcgtg gtgggcccga ccctgcgcct gcaccagtgc
     3601 ggcctgccca ag
//
LOCUS       DASUEG010000212         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_734371, whole genome shotgun sequence.
ACCESSION   DASUEG010000212 DASUEG010000000
VERSION     DASUEG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734371"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1628
                     /gene="yidC"
                     /locus_tag="VFX11_04025"
     CDS             3..1628
                     /gene="yidC"
                     /locus_tag="VFX11_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4937817.1"
                     /translation="MTWQKDYGAKPATEAVAATTATPVAGNTDTPTAPAAPANGDVPA
                     VPTAAASTDAPVDSAVQTSGLVNVKTDVLDLQIDTRGGDIVRAALPQYTESIDSKKSF
                     VLMDHSAARTYIAQSGLIGANGPDAAAAGRPQYQVTARDFVLADGKDTLAVTLVLPQE
                     NGVEIHKVFEFTRSNYLVKVRYDIINRGTAPWNGLMYAQLKRDNSEDPSASNQGFGMM
                     TFLGGAWWTKEKPYNKVALDDFAEEPLKEAVTGGWAAMIQHYFVSAWIPAKDSANVYT
                     TRKSGNDNIIGYTGPQVTVAPGTQQKIEASLYVGPKIQKVLEEVSPGLELTVDYGWLW
                     PVSQFLFWLLTMIHGWIGNWGWAIVLLTVLVKAAFFQLSATSYKSMAKMRTVMPEMQR
                     IKEQHAGDRAKQSQAMMDLYKKEKINPLGGCLPILVQMPVFIALYYCLMESVELRHAP
                     WLGWIRDLSVMDPYFVLPLIMGVTMWVQQHLNPAPPDPMQAKVMKIMPIVFTVMFLWF
                     PAGLVLYWVVNNTLSILQQWVITRQIEKAAGKA"
     gene            1693..>2054
                     /locus_tag="VFX11_04030"
     CDS             1693..>2054
                     /locus_tag="VFX11_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX4937818.1"
                     /translation="MMKNTDTIAAIATPPGRGGVGIVRVSGPKAQAIGQAILQRETSL
                     PVRRALFGGFHAADGSEIDEGLALFFQGPQSFTGEDVIELQGHGGPVVMDMLLQRVLA
                     LGARMARPGEFSERAFLN"
BASE COUNT          424 a          580 c          634 g          416 t
ORIGIN      
        1 tcctgacctg gcaaaaggac tacggtgcca aacctgccac tgaggccgtg gccgccacta
       61 ctgcaacacc ggttgccggc aacaccgata cgcccaccgc accggccgcc cccgccaatg
      121 gtgatgtccc cgctgtgccg acagcagcag ccagcacgga tgcaccggtc gattccgctg
      181 tgcagacgag tggtctcgtc aatgtcaaaa ccgatgtgct cgatctgcag attgacaccc
      241 gcggtggtga catcgtgcgt gctgcgctgc cgcagtacac cgagagcatc gacagcaaaa
      301 aatctttcgt gctgatggat cattccgctg cacgcactta tatcgcacag agtggcttga
      361 tcggtgccaa tggcccggat gccgctgctg ccggtcgtcc gcagtatcag gtaacggcgc
      421 gtgatttcgt gctggctgat ggcaaggaca cactcgccgt gacgctggtg ttgccgcagg
      481 aaaacggcgt cgaaattcac aaggtcttcg aattcacacg cagcaattat ctggtgaagg
      541 tgcgctacga catcatcaac cgcggcaccg caccgtggaa cggcctgatg tatgcgcagt
      601 tgaagcgtga caacagtgaa gacccaagcg ccagcaatca gggcttcggc atgatgacct
      661 tcctgggcgg tgcctggtgg accaaggaaa agccgtataa caaggttgcg ctggatgatt
      721 ttgccgaaga accgttgaag gaagcggtga ccggcggttg ggctgcgatg atccagcatt
      781 atttcgtgag tgcctggatt ccggccaagg acagtgccaa cgtttacacc acccgcaagt
      841 ccggcaatga caacatcatc ggctacaccg gcccgcaagt gaccgtggca ccgggcacac
      901 agcagaagat tgaagcctcg ctgtatgtgg gcccgaaaat ccagaaggtg ctggaagaag
      961 tgtcgccagg tctggagctg acggttgatt atggctggct gtggccggtg tcgcagttcc
     1021 tgttctggct gctgaccatg atccacggct ggattggcaa ctggggttgg gccatcgtgc
     1081 tgctgaccgt gctggtaaag gccgcgttct tccagctctc cgccaccagc tacaagtcca
     1141 tggccaagat gcgtaccgtg atgccggaaa tgcagcgcat caaggaacag catgccggtg
     1201 accgtgccaa gcagtcgcag gcaatgatgg acctgtacaa gaaggaaaaa atcaatccgc
     1261 tcggcggctg tctgccgatc ctggtgcaga tgccggtgtt cattgcgctg tattactgcc
     1321 tgatggaatc ggtcgagttg cgtcatgcac cgtggctggg ctggattcgc gacctgtccg
     1381 tgatggaccc gtatttcgtg ttgccgctaa tcatgggtgt gacgatgtgg gtgcagcagc
     1441 atctgaatcc ggcaccgcca gatcccatgc aggccaaggt gatgaagatc atgccgatcg
     1501 tgttcaccgt gatgttcctg tggttcccgg ccggtctcgt gctgtactgg gtggtgaaca
     1561 acacgctgtc catcctgcag cagtgggtga ttacccgtca gatcgagaag gcggcgggca
     1621 aggcctgatc gccacctgaa cgctgcatca caaaaaggct cctgacggag cctttttgct
     1681 accatggatg ccatgatgaa aaacaccgac accattgccg ccattgccac accaccggga
     1741 cgcggaggtg tagggattgt gcgcgtgtcg ggaccgaagg cgcaggccat cggccaggcc
     1801 attctgcaac gtgagacttc gctgccggta cgtcgcgcct tgtttggcgg ttttcatgcg
     1861 gccgatggca gcgaaatcga cgaagggctg gcgctgtttt tccagggccc gcaatcgttc
     1921 accggtgaag atgtcatcga gttgcagggt cacggtggtc cggtggtaat ggacatgctg
     1981 ttgcagcgcg tgctcgcgct gggcgcgcgc atggccaggc ccggggaatt ttccgagcgc
     2041 gcctttctca acga
//
LOCUS       DASUEG010000213         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1573741, whole genome shotgun sequence.
ACCESSION   DASUEG010000213 DASUEG010000000
VERSION     DASUEG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573741"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFX11_04035"
     CDS             complement(<1..180)
                     /locus_tag="VFX11_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937819.1"
                     /translation="MSTARLHSDTVAFFDEFTAVFPTFDGAQIALRYAAPYLAIHADG
                     SSDLFPTTESIASYFQ"
     gene            complement(190..795)
                     /locus_tag="VFX11_04040"
     CDS             complement(190..795)
                     /locus_tag="VFX11_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX4937820.1"
                     /translation="MPLEFWFEFASTYSYPAAMRIQSVAARQGVSILWRPFLLGPIFK
                     DSGWSNSPFNLYPAKGRYMWRDLERICDDLGLPFRRPTVFPRNGLLAARIACQFADAP
                     WLPTFALAVYRANFERDLDIAEAATLADCLQQAGQDPMAILATAQLPEAKEKLRKQNE
                     RAVQLGIFGAPSFVAGNELFWGNDRMENAMRWCKEHQICQD"
     gene            complement(829..1872)
                     /locus_tag="VFX11_04045"
     CDS             complement(829..1872)
                     /locus_tag="VFX11_04045"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX4937821.1"
                     /translation="MSVNQLSAAGARLPSAWSDARRLLFCLLALLASDGRAQDLMAYR
                     SPQPTQSTVPAGTAADTTGPLNPFRLQPGDHEFKLESDRKYLLHIPAGYSPAKPAPLV
                     LFFHGGGGHMEQAADAYGWREKSDKEGFIVAFPNGTSRFPRQHLATWNAGNCCGSARD
                     KNVDDVGFVRQVIADIQRQVPIDRARIFATGMSNGGMMSYRLACEAADIIRAVAAVAG
                     TDNTQTCKPSQPVSVLHIHARDDTHVLFDGGAGKDAFRDESKVTDFTSVNDTIRRWIA
                     LNHAAGTPRRVLDVPGAIGDLYTSKQNSARIQLVVTETGGHSWPGGKAVRRKKPSTAI
                     NATDLIWDFFQSQ"
     gene            complement(1893..2537)
                     /locus_tag="VFX11_04050"
     CDS             complement(1893..2537)
                     /locus_tag="VFX11_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937822.1"
                     /translation="MPEHYSLNIRGYTVDFHVYVTVPNSDVKVQQLQRFFARMPDQHL
                     AVLYPIFVMERKPGGRAGGGTWPPGLVRSQVMGAGHARNTGIPDADVERLVVSRASGM
                     IGLSRDRWERPIARLPFTVFHEVGHCVDIALGLVPGGATEAEFAGMATDRCGAGNMMA
                     RRVVEAYARCICSPNAIYHTLPPRETPASANQRLLGALRRSAAFRTVPAAWQPR"
     gene            complement(2616..3173)
                     /locus_tag="VFX11_04055"
     CDS             complement(2616..3173)
                     /locus_tag="VFX11_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937823.1"
                     /translation="MDGINRSLPGQETGIVYKRNSAWWFATCYLLGSFFLLLLTVILL
                     FGSVLWLGKEGVVALKQASDEQEVERAISGAVRDHIKLAGFKVTSRLDMATLSVSIRN
                     ESKYAIEDVKVEIAHLDANGSPVFVRDEPLRDLRTIFPGDTAHSQIDFQLRPEYQDSS
                     YSVRISGFKVMGDAVLKEFCKQPQS"
     gene            complement(3184..>3514)
                     /locus_tag="VFX11_04060"
     CDS             complement(3184..>3514)
                     /locus_tag="VFX11_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX4937824.1"
                     /translation="VEAAVSDRMLKAQISEEQQRMVGYGTFERAVDVLAQAVSAHPYV
                     AGPTFTAADVYVGSHVIWGMQFGSLPKRPEFEAYAAKLVVRPAYKAAKAIDEKLGAAL
                     AARQGKE"
BASE COUNT          740 a         1094 c          990 g          690 t
ORIGIN      
        1 ctggaaatag gaggcgatgg actcagtggt gggaaacagg tcactgctgc cgtcagcgtg
       61 gattgcaagg tagggagcag cataacgcag ggcgatttgt gcaccgtcga aggtgggaaa
      121 aacagcggtg aattcgtcga agaatgctac ggtgtccgag tggagtctgg ctgtgctcat
      181 cgtcgtgatt caatcctgac agatttgatg ttctttacac cagcgcatgg cattttccat
      241 gcggtcgtta ccccagaaaa gctcgttgcc tgccacaaac gacggcgcgc caaaaatgcc
      301 cagctgcacg gcccgctcgt tttgtttgcg caacttttct ttcgcctccg gcaattgggc
      361 tgttgccaga attgccatcg ggtcctgccc cgcctgctgc aggcaatccg caagcgtggc
      421 tgcctctgca atgtccagat cccgttcgaa attggcacgg taaacggcca gcgcaaaagt
      481 cggcagccag ggggcatcgg caaactgaca ggcgatccgc gccgccagca ggccattgcg
      541 cgggaaaacg gtcggtcgac ggaacggcaa tccaaggtcg tcacagatcc gttccagatc
      601 ccgccacata taacggccct tcgcaggata aagattgaag ggggaattgg accagcccga
      661 atccttgaaa atggggccca gcagaaacgg gcgccagaga atgcttacgc cctgccgcgc
      721 ggctacgctc tgaatacgca tcgcggcggg ataggaatag gtgctggcaa attcaaacca
      781 gaattccagc ggcatgaccg ggctccatta cgctattgca ctgcatcatt actgactctg
      841 aaaaaaatcc cagatcagat ctgtggcgtt gatcgcggtg gaaggctttt tccttcgcac
      901 ggccttgccg cccggccagg aatggccgcc ggtttcggtc accaccaact gtatccgcgc
      961 gctgttctgc ttggaagtgt acagatcgcc aatcgcgccc ggcacgtcca gcacgcggcg
     1021 tggggtccct gcggcatggt tcaatgcaat ccagcgcctg atggtgtcgt ttacggacgt
     1081 gaagtcggtc accttcgatt catcccggaa tgcatccttc cccgcaccgc cgtcgaacag
     1141 cacgtgcgta tcatcccggg cgtggatatg cagaaccgaa acaggttggg acggcttgca
     1201 ggtttgcgtg ttgtcggtac cggcgaccgc tgccaccgct ctgataatat ccgccgcctc
     1261 gcacgccagg cgataagaca tcatgccgcc attggacatg ccggtggcaa aaattctggc
     1321 tctgtcgata gggacctgtc gctggatatc ggcaatgacc tgtcgcacaa atcccacatc
     1381 gtccacattc ttgtcgcggg cagagccaca acagttgccc gcgttccagg tggccagatg
     1441 ttgccgggga aaacggctgg tgccgttggg aaaagctacg atgaatcctt ccttgtccga
     1501 cttctcgcgc cagccgtacg cgtcagcggc ctgctccatg tgcccgccac caccgtggaa
     1561 gaacagtacc agcggcgccg gtttcgctgg cgagtagccc gccggaatat gcagcaggta
     1621 ttttctgtcg ctttccagct tgaactcatg atcacccggc tgcagcctga atgggttgag
     1681 agggcctgtg gtgtccgccg cagtgcctgc gggtacagtg gactgcgttg gctgcgggga
     1741 tcgataggcc atcaggtcct gcgcccgacc atcgctggcc agcaatgcca gcaggcagaa
     1801 caatagtcgg cgagcatcac tccatgcaga aggaaggcgc gcgcccgctg cggacagttg
     1861 attgacgctc acggcatgcc tcctgaaatt cgtcagcgtg gctgccacgc cgcgggtacg
     1921 gtgcggaatg cagccgagcg gcgcaacgcg cccagcagtc gctgattggc actggccggc
     1981 gtttcccggg gcggcagcgt gtggtagatc gcgttgggcg aacagatgca gcgggcatag
     2041 gcttccacca cgcgccgtgc catcatgttc ccggcaccac agcggtcggt ggccatcccc
     2101 gcgaactccg cttcggtggc gccacccgga accagcccca gtgcaatatc aacacagtgg
     2161 cccacttcat gaaacaccgt gaaaggcagc cgcgcaatcg gccgctccca gcggtcgcgc
     2221 gaaaggccaa tcatcccgct ggcccgcgac accaccaacc gttccacatc cgcatccggt
     2281 atgccggtgt tgcgggcatg gcccgccccc atcacctggc tgcgcaccag tccgggtggc
     2341 caggtaccgc ctccggccct gccaccgggt tttctttcca tcacaaaaat cggatacaga
     2401 actgcaaggt gctgatccgg catgcgggca aagaaacgct gcagctgctg cacctttaca
     2461 tccgaattcg gcacagtgac atacacatga aaatccaccg tataaccgcg aatgttcaga
     2521 ctgtaatgtt cgggcatgag gactccttat cgggcgagct tcgggatcgc cgccagtaac
     2581 cgggtagaaa gggtcacata cggcgacccg tccggttatg attgcggctg tttgcagaat
     2641 tccttcagca cggcatcgcc catcaccttg aaacctgaaa tgcgcaccga atagctggaa
     2701 tcctgatact cgggcctgag ctggaaatca atctggctgt gcgcggtatc gcccgggaag
     2761 atcgtgcgca aatcccgcaa tggctcatcg cggacaaaaa cgggcgaacc attggcatcc
     2821 agatgggcga tttccacctt cacatcctca atggcgtact tgctttcgtt cctgatcgat
     2881 accgacagcg tggccatgtc caaccgggag gttaccttga accccgccag cttgatgtgg
     2941 tccctgacag cgccggagat tgcgcgctcg acttcctgtt catcgctggc ctgcttcagc
     3001 gcaaccacgc cctccttgcc caaccacagc accgagccaa acaacaggat aacggtgagc
     3061 agcaacagaa aaaaactgcc cagcaggtag caggttgcaa accaccaggc cgaatttcgc
     3121 ttgtagacga ttccggtttc ctgcccggga agactgcgat tgattccatc cataatctgc
     3181 gaattattcc ttgccttgcc tggcggccag tgccgcaccg agcttctcat caatggcctt
     3241 ggcggccttg tatgccggtc gtactaccag ttttgccgca taggcttcaa attccggacg
     3301 cttcggcaac gagccaaact gcatgcccca gattacatgc gatcccacat acacatccgc
     3361 cgccgtgaac gttggcccgg ccacataagg atgcgcgctg acagcctgcg ccagcacatc
     3421 cactgcgcgt tcaaacgtgc cataccccac catgcgctgc tgctcttcgc tgatctgggc
     3481 cttcagcatg cgatccgata cagccgcctc cacc
//
LOCUS       DASUEG010000214         5627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_28011, whole genome shotgun sequence.
ACCESSION   DASUEG010000214 DASUEG010000000
VERSION     DASUEG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28011"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1225
                     /locus_tag="VFX11_04065"
     CDS             266..1225
                     /locus_tag="VFX11_04065"
                     /inference="COORDINATES: protein motif:HMM:NF036558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937825.1"
                     /translation="MKPFSRLFFQRFLTVSLLSAGTAAPALAQAPFDLKVHSGMGNET
                     GNLQWSIADDPTGATGPNVLSELTYRDLQFSVFHASGELAFNHGALRNTVLFLDYQTG
                     QATDGEVQDSDYNGNNRTREYSRSLSSAEESSLDGFTVGLGYRFRINQYNALTPMAAY
                     TRQNQDLVMTQGQQVVDAYNPNNLGAFRGTLNSTYLTDWTGAWAGVRWNLETRAHTLA
                     LTLKNFWMDYHAEADWNLRNDFAHPKSFEHWATGGGTGVDLSYQYRLSNNFSLRADWY
                     QQNWETGTGQDTVYFANGTTGGSQLNEVSWESTGFSMGIQLEI"
     gene            complement(1230..1547)
                     /locus_tag="VFX11_04070"
     CDS             complement(1230..1547)
                     /locus_tag="VFX11_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX4937826.1"
                     /translation="MSVQSTIQSKLQSRFQPRHLEVINESFMHSVPPGSETHFKVVVV
                     SDAFDGKRSVARHQAVYQALADEVAGPVHALAIHTYTGAEWEAVAAAPDSPQCRGGSK
                     HDH"
     gene            complement(1608..4253)
                     /locus_tag="VFX11_04075"
     CDS             complement(1608..4253)
                     /locus_tag="VFX11_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4937827.1"
                     /translation="MQAKKVWFWGLSVWVLCLVLGSAWPGRVLASGSLVMADSQPRIL
                     LSGRTAGELDISNYLEVLPSADPRLDLQAVLSGDLPFQPIDNNGISTAPGANLYWIRG
                     SMSRSLADLSGPGSWLLILENTRVRQAQLYVRQSAGLPGADLAAPYLPVERIPGERYP
                     QFALTLSPDSTLDFYLRLETDGLFSIPVKVWARDAYLLSKRSQLPVWGLLFGAISALT
                     LYNLFIYLSLRERAYLQLSLFLLSTLLLTLAREGFLVTGLSRMPLIGDRFEAVAEFVT
                     LALAILFTRSYLNTARYYPAIDAVLRANRWVCMGFAFAVLAGLLSTTQSFAGLLVSGA
                     LFFVPAVLASMHAADRATRYYIAGWSVFFVGYMIYLASQLGLLPVNDFTQRGRDLALC
                     FLGLSLSLGMAEQIRRERFEKQRALQRQQDTMLELKYSEEQIQKKVLRDTLQEFPGLP
                     TLQERLETVVDPSMQSGESVILVMMDLHNVNRVEEQLGHAARNELLTRATKRLSVILR
                     SVSGVLPLTESAADYVPMAVLEDGSYGFILRGVSDITINHAIEQVEQAMSRPFFYQGL
                     ELQPGISFGLARLGEHGEDADALLLHAERSLMVDRRKNLDKVCELDGVDQYNERNIAL
                     INELRSAIHEDQITLYFQPVYDLRRNLACGVEVFTRWESLSGARISPGEIFHLAEIGG
                     FVSELTLRVIEKALRHFMVAVNVQQSTLKLSVNLSPRCLREEHFLEEVDLMLTRYGLP
                     PQRLSLEIKEAAIIEDPSITREVLNRIRNLGIGLTIDELGAAYSNPSYLSSLPVTEVK
                     LDQSFVARLQEADCRVMVLMLIELCREQHIKLVVHGVEEESTLQQLELMGCSFAQGHY
                     LAAPVQAREFKLPRHQLTPLQFQQV"
     gene            4530..4838
                     /locus_tag="VFX11_04080"
     CDS             4530..4838
                     /locus_tag="VFX11_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937828.1"
                     /translation="MLLMPRIHRRSIERLKSNRSIRVYESERIHPVGQEIKPTYSERR
                     LVRERRKQEVPVQEDRRRQPRRNAHQNLRPEIRALLQNSGDRNAATIRRDGVYINEDV
                     "
     gene            5050..>5627
                     /locus_tag="VFX11_04085"
     CDS             5050..>5627
                     /locus_tag="VFX11_04085"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX4937829.1"
                     /translation="MKQPVRVVVTGAAGQISYSLLFRIAAGQMLGADQPVILQMLEIT
                     PALGALQGVAMELDDCAFPLLAGMVVTDDPKVAFKDADYALLVGARPRGPGMERKDLL
                     EANAAIFSVQGKAIDAVAKRSIKVLVVGNPANTNSLIAQRNAPGINPRQFTAMTRLDH
                     NRALSQLATKTGTSINDITKVTIWGNHSSTQYP"
BASE COUNT         1302 a         1780 c         1491 g         1054 t
ORIGIN      
        1 actttttata caaacctttt ccaagccaaa tcctgcaatt gatcacttaa taaacaaacg
       61 aaggctcaaa tgccaatctt ttgacagccc ccggttacaa ttttttttat cccgctaagt
      121 gattgatgtt caatatcaag cgtattttca atgagttacg aacacttttg caggaaaaat
      181 cccgtctacc ctgaaaagga acttcgggtg atctgttgct gttccccggc tttgatgaac
      241 ctgcttcagt gagtcggtct gccttatgaa accttttagc cgcctgtttt tccagcgttt
      301 tctgacagta tccctgctga gcgccggcac cgccgcgccg gcactggcgc aagcgccgtt
      361 tgatctcaag gtgcattccg gcatgggcaa tgaaaccggc aatctgcagt ggagcatcgc
      421 cgatgacccc actggtgcaa ccggacccaa tgtactgtca gaactgacct accgcgatct
      481 gcaattcagc gttttccacg cgagcggtga actggctttc aaccacggcg ctttgcgcaa
      541 taccgtgctg tttctggact accagaccgg tcaggccacc gacggcgaag tacaggattc
      601 ggactataac ggcaacaacc gcacccggga atacagccgc agcctgtcat ctgccgagga
      661 atccagtctg gatggcttca ctgtgggcct gggctatcgc ttccgcatca accagtacaa
      721 cgcactgacc cccatggccg cctacacccg ccagaaccag gatctggtaa tgacgcaagg
      781 ccagcaggtg gtggacgcat acaatccgaa caacctcgga gccttccgcg gcacactcaa
      841 cagcacctac ctgacagatt ggaccggcgc ctgggcaggt gtgcgctgga atctggaaac
      901 ccgtgcgcac accctggcac tgaccctgaa aaatttctgg atggattatc acgccgaagc
      961 ggactggaac ctgcgcaatg atttcgccca cccgaaaagt ttcgagcact gggcaaccgg
     1021 cggcggtacc ggcgtcgatc tgtcgtacca ataccggctt tcgaacaatt tctccctgcg
     1081 ggcagactgg tatcagcaga actgggaaac cggcaccggc caggacaccg tgtatttcgc
     1141 caacggcacc accggcggca gccagctgaa cgaagtcagc tgggagtcca ccggcttctc
     1201 aatggggatt caactggaga tatgagacgt cagtggtcgt gcttgctgcc accccggcac
     1261 tggggtgaat ccggcgcggc cgctactgct tcccactctg cgccggtgta ggtatgaatc
     1321 gccagcgcat gcaccggccc tgccacttca tcggccaggg cctggtaaac cgcctgatgg
     1381 cgcgccaccg agcgcttgcc gtcaaacgca tccgacacca ccaccacctt gaaatgggtt
     1441 tctgaacctg gcggcacgct gtgcatgaaa ctttcgttga tgacttcaag atgccggggc
     1501 tgaaagcggg actggagttt ggactggatg gtggactgga cggacatggg cacacctgcg
     1561 aaggacgact gcggacgttc cctatattac gccttcaaac ccggtggtca aacctgctga
     1621 aattgtagcg gcgtcagctg gtgacgaggc agtttgaatt cgcgcgcctg taccggcgcc
     1681 gccagatagt gtccctgggc aaaactgcat cccatcagtt ccagctgctg caaggtggat
     1741 tcttcctcca ccccgtgcac caccagtttg atgtgctgtt cccgacacaa ctcgatcagc
     1801 atcagcacca tcacccggca atccgcttcc tgcaaccgcg caacaaaact ctggtccagt
     1861 ttcacttcag tcaccggcag gctgctgaga taagacggat tgctgtaggc cgcacccagt
     1921 tcatcgatgg tcaggccgat gcccagattg cggatgcgat tgagcacctc gcgggtgatg
     1981 ctgggatctt caatgatggc cgcttccttg atttccagcg acaaccgctg cggcggcaga
     2041 ccataacggg tcagcatcag atccacctct tccagaaaat gctcttcccg cagacagcgc
     2101 ggcgacaggt tcactgacag tttcagtgtc gactgctgca cgttcaccgc caccatgaag
     2161 tgtcgcagcg ccttttcaat cacgcgcaag gtgagctccg atacgaagcc gccaatttcc
     2221 gccagatgaa agatttcgcc cgggctgatt ctggcaccac tgaggctttc ccagcgggta
     2281 aacacctcga cgccacaggc cagattgcgg cgcaggtcgt acaccggctg gaaatacagg
     2341 gtgatctgat cctcgtgaat ggccgagcgc agttcattga tgagcgcaat gttgcgttcg
     2401 ttgtactgat ccacgccgtc cagctcacac accttgtcga gattcttgcg gcgatccacc
     2461 atcagggaac gctcggcgtg cagcagcaaa gcatccgcat cctcaccatg ctcacccagc
     2521 cgcgccaggc caaacgagat gcccggctgc aactccagcc cctgatagaa gaacggccgc
     2581 gacatggcct gctccacctg ctcgatggcg tggttgatgg tgatgtcgga aacaccccgc
     2641 aggataaagc cgtaactgcc gtcttccagc acggccatgg gcacgtagtc ggcagcactt
     2701 tcggtcagcg gcaacacgcc actcacactg cgcagaatca cactcaggcg cttggtggcc
     2761 cgcgtcagca attcattgcg ggcagcgtgc ccgagctgct cttccacccg gttcacgtta
     2821 tgcaggtcca tcatcaccag aatgacggat tcaccggact gcatggatgg gtccaccacg
     2881 gtttccaggc gttcctgcaa cgtcggcaat ccgggaaatt cctgcagggt gtcgcgcagc
     2941 acttttttct ggatctgttc ttcggaatat ttcagttcca gcatggtgtc ctgctggcgc
     3001 tgcagggcac gctgcttttc gaagcgttca cggcgaatct gctcggccat gcccagcgac
     3061 agtgacaggc ccagaaagca cagtgcgagg tcacggccac gctgggtgaa atcattcacc
     3121 ggcaacaatc ccagctggct tgccagataa atcatgtagc ccacaaaaaa caccgaccag
     3181 ccggcaatgt aataacgggt ggcacggtct gccgcgtgca tggacgccag taccgccggc
     3241 acaaagaaca gcgcgccact caccagcaat ccggcgaagg actgggtggt tgacaacaag
     3301 cccgccagca cagcaaaggc gaaccccatg cagacccagc gattggcacg caacacagca
     3361 tcgattgccg ggtaatagcg cgcagtattc agatagctgc gcgtgaacag gatggccagc
     3421 gccagcgtca cgaattccgc cacggcttcg aagcggtccc caatcagggg catgcgggaa
     3481 agcccggtaa caaggaatcc ttcccgggcc agcgtcagca acagcgtgga cagcagaaac
     3541 aggctcaact gcaggtatgc ccgttcccgc aatgacaaat agatgaacag gttgtagagc
     3601 gtcaatgcgg agatggcgcc aaacaacagc ccccagaccg gcagctggct gcgcttgctg
     3661 agcagatagg catcgcgggc ccagactttc accggaatac tgaacaggcc atcagtttcc
     3721 agccgcaagt agaaatcgag ggtcgaatcc ggcgacaggg tcagcgcaaa ctgcggataa
     3781 cgctcccccg gtatccgctc cacgggaaga tagggggccg ccaggtcggc tccgggtagt
     3841 ccggcgctct gccgcacata aagttgtgcc tgccgcacgc gggtattctc cagaatcagc
     3901 agccagctgc caggaccgct cagatcggcc agcgaacggc tcatgctgcc acgaatccag
     3961 tacaggttgg cgcccggcgc ggtggagatg ccgttgttgt ctatcggctg gaagggcaaa
     4021 tccccggaca gaaccgcctg cagatccaga cggggatctg ccgatggcag cacttccagg
     4081 taattcgaga tatccagctc accggccgtg cggccactga gcagaatgcg gggttgggaa
     4141 tccgccatca ccagggaacc ggacgccagc acccgccccg gccatgcgct ccccaggacc
     4201 agacacaaca cccacaccga caatccccaa aaccagacct tctttgcctg cataacggca
     4261 aagcctgtag cgaccattac gagcacccat caaaatcagc caccctccct ggtgaccagc
     4321 ataagacgat aaatacctaa cccggcttaa aaaatagcct ctgaatccaa aattgtctaa
     4381 caacattaga gagaatcgtt aacaatcggg aacaaacgca cacgcaatct gcacgaaatg
     4441 ccgctttacg atcaaaacgt tgattcgtaa agtttttcac aagagtgccg ataatccagt
     4501 tgagccctcg aaaaccaccg gagcggacca tgcttttaat gccccgcata catcgacgat
     4561 cgatcgaacg gctgaagtcc aaccgttcca ttcgcgttta tgaatccgaa cgcatccatc
     4621 cggtcggcca ggaaatcaaa cccacctact ctgaacgccg cctggtgcgc gaacgccgca
     4681 agcaggaggt gcccgttcag gaggaccggc gccgccagcc ccgtcgcaat gcacaccaga
     4741 atctccgccc cgaaatccgc gccctgttgc agaattccgg ggaccgtaat gccgccacca
     4801 tccgccgtga cggtgtttat atcaacgagg acgtctgatt cagggcacgt caatcacggg
     4861 caccctgagt cgccgataaa aatccgccag gctgcagcat cccgctcatc ttgcgcccgc
     4921 accggacggg ttccggtatt ccaacgacct tcactgacct ccaaacggcc tgcaaatctg
     4981 atggtatttg ccattgcaga tgcatagaat tgtgcggtcc taaaaccagg ctaacaggag
     5041 atcccactca tgaaacaacc tgtacgtgta gtcgttaccg gtgcagcagg ccagatcagc
     5101 tattcccttc tcttccggat tgccgccggc cagatgctcg gcgccgatca acccgtcatt
     5161 ctgcaaatgc tggaaattac cccggccctg ggcgccctgc aaggtgtagc catggaactg
     5221 gacgattgcg cgttcccgct gctggcgggc atggtggtca ccgatgatcc gaaagtggca
     5281 ttcaaggatg ccgactacgc cctgctggtc ggtgcccgtc cccgcggccc cggcatggag
     5341 cgcaaggacc tgctggaagc caacgctgcc atcttctcgg tacagggcaa ggcgatcgac
     5401 gccgttgcca agcgcagcat caaggtgctg gtagtgggca accccgccaa caccaactcg
     5461 ctgatcgccc agcgcaatgc gcccggcatc aacccgcgcc agttcaccgc catgacacgc
     5521 ctggaccaca accgcgccct gagccagctg gcgaccaaaa ccggcaccag catcaacgac
     5581 atcaccaagg tcaccatctg gggcaaccac tcctccaccc agtaccc
//
LOCUS       DASUEG010000215         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_189599, whole genome shotgun sequence.
ACCESSION   DASUEG010000215 DASUEG010000000
VERSION     DASUEG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189599"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..551
                     /locus_tag="VFX11_04090"
     CDS             3..551
                     /locus_tag="VFX11_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008483700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX4937830.1"
                     /translation="MLIYLFDAYIMVVFTRFLLQLLRADFYNPVSQFVVKATTPVLKP
                     LRRVIPGVGGLDNASLVLIFALIVAKLLLWSFINFGALAPLPDLAILLLKCTALLLIN
                     YFFMILLVSAVMSWVVQGYHPVAAVLFQLSDPIVAPVRRLLPAMGGIDFSPMVVMLGL
                     YVVRILFQLDMAFVAGIPVQVP"
     gene            567..860
                     /locus_tag="VFX11_04095"
     CDS             567..860
                     /locus_tag="VFX11_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX4937831.1"
                     /translation="MSWYRWDGDDLILDCRLQPKASSDELVGDLGDELKIRITAPPVD
                     GKANAHLVQFLAKVFGVSKSRVTIEKGELGRSKRVRIQAPGKFPANLNIIKPC"
     gene            949..2940
                     /locus_tag="VFX11_04100"
     CDS             949..2940
                     /locus_tag="VFX11_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX4937832.1"
                     /translation="MDSARLSSQMDAYLQWRQEIFRELTRYRAWLNEHHVQSPDLENK
                     LQQAMQTLKDDRITIAFVGEFSRGKTELINALFFAHYGTRILPSGAGRTTMCPTELFF
                     DHRAQDSYIRLLPIETRMVGSSIASFRKIPEKWIFVPLNAKDPKMMKKAFAEVANSKA
                     VSPNEAAAMGFQVELLEPAPEKPDKVLIPAWRHAMISFDHPLLRQGLTIIDTPGLNAL
                     GCEPELTFSLLPEAQSILLLLSASTGVSATDLDIWNRHIRDLALRESTGVYAVLNKID
                     AVWDELESDAYNQKALGTIRAQCARQLSIAPDEILTVSAKEGLLAQVGGNQARLQKSQ
                     LPALEELLSNDMMRRKELLIQKSVIRDIHAMLHGSKKTLLRRRENALEELKIMKSDET
                     NRKEQLDNLVTQAKAEQGFYHKKLILLKTSRKMIEREGALMLEPVTQARLDTYLKEAG
                     KALTSSWTIVGMNNAIDTFFDRLKADMSALAQKQEQIQIMVADIYARYEAEQGLAPLD
                     YPRFSPKEYLTRLDDLRAKSGSFRRNLRKLLTEQKSTSKRFLATVVNEGSSIYRLYER
                     SGRDWLNNVLMPLFQNAQEQKHLLDEHVQKLRLMASVDGNAAERVGQMEAMLADVETQ
                     IAEVEDMIKGLRKPAPIHQQRKVIPLITAQFSPPAPVKK"
     gene            complement(2950..>3625)
                     /locus_tag="VFX11_04105"
     CDS             complement(2950..>3625)
                     /locus_tag="VFX11_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4937833.1"
                     /translation="NAACVVDKDWDIDDAVKRIIIGAFYQSGQSCISVQRIYAHADIY
                     EQLKAKLVAATRQLKSGDPKNPDTFIGPMISEGEAKRLHGWINEAIESGATLLCGGQR
                     KGNMLEATLLENVDSQQNACAEEAFGPLAILSKFTDFDQVLKDVNNSRFGLQAGIFTR
                     DLYKAHKAWDELEVGGVVIGDVPSWRVDHMPYGGVKDSGLGREGIRWAMHDMTELRLM
                     VMRTPQ"
BASE COUNT          733 a         1092 c         1077 g          723 t
ORIGIN      
        1 tcattctgat ctaccttttt gacgcttaca tcatggtggt gttcacccgt ttcctgctgc
       61 agctgttgcg ggcggatttc tacaatccgg tatcccagtt cgtggtgaag gccaccacgc
      121 cggtgctcaa acccctgcgt cgcgtcatcc ccggcgtagg tgggctggac aacgcgtccc
      181 tggtgctgat atttgccctg atcgtggcca agctgctgct gtggagtttc atcaacttcg
      241 gggccctggc gccactgcca gacctggcga tcctgctgct gaaatgcacg gcgctgctgc
      301 tgatcaacta tttcttcatg atcctgctgg tcagcgcagt gatgagctgg gtggtgcagg
      361 gttatcaccc ggtggccgct gtcctgttcc agctgtccga tcccatcgtc gcgccggtgc
      421 ggcgtctgct gccggccatg ggtggcatcg atttctcccc catggtggtc atgctcgggc
      481 tgtatgtcgt gcgcatcctg ttccagctgg acatggcgtt cgtggccggc attccggtgc
      541 aagtgccctg agcggcggcg tccggcatga gctggtaccg ctgggacggc gatgacctga
      601 tcctggactg ccgtctgcaa cccaaggcgt cctccgatga actggtgggc gacctgggtg
      661 acgaactgaa aatccgcatc accgcaccac cggtggacgg caaggctaac gcccaccttg
      721 tacagtttct ggccaaggtg ttcggtgtca gcaaaagccg cgtgaccatc gaaaaaggtg
      781 aactggggcg cagcaaacgg gtgcgcattc aggcacctgg caaatttcct gctaatctga
      841 acataatcaa gccctgttag ctcaaccggt tagtcagggc gggggccctg ctttccggga
      901 aggcatggcg ttcgtgagca gcattggggt ttgagcgggc ttcgggcaat ggattcggcg
      961 cgtctttcca gtcagatgga tgcctatctt cagtggcggc aggaaatctt ccgcgaactg
     1021 acccgttacc gtgcctggct gaatgagcac catgtgcaga gcccggacct ggagaacaag
     1081 ctccagcagg ccatgcagac gctcaaggac gacaggatca ccatcgcctt tgtgggcgag
     1141 ttctcccgcg gcaagaccga gctgatcaac gctctcttct tcgcccatta cggcacccgc
     1201 attttgccgt cgggcgccgg ccgcaccacc atgtgtccca ccgaactttt cttcgaccac
     1261 cgtgcccagg acagctatat ccgcctgctg cccatcgaaa cccgcatggt gggctccagc
     1321 attgccagct tccgcaagat tcccgaaaaa tggatcttcg tgccgctcaa cgccaaagac
     1381 cccaagatga tgaaaaaggc gttcgcggaa gtggctaaca gcaaggcagt cagccccaat
     1441 gaagcggctg cgatgggctt tcaggtggaa ttgctggaac cggcgccgga aaagcccgac
     1501 aaggtgctga tcccggcctg gcgtcacgcc atgatcagtt tcgatcaccc gctgctgcgg
     1561 caggggctca ccatcattga tacgccggga ttgaatgcgc tgggctgcga gccggaactg
     1621 accttcagcc tgctgccgga agcgcaatcc attttgctgc tgctgtcggc gtccaccggt
     1681 gtcagcgcca ccgatctgga tatctggaac cgtcatatcc gcgatctggc gttgcgcgaa
     1741 tccaccggcg tgtacgcggt gctgaacaag atcgatgcgg tgtgggatga actggaaagc
     1801 gacgcctaca accagaaggc cctcggcacc attcgcgccc agtgcgcgcg ccagctgtcg
     1861 atcgcgccgg acgaaattct tacggtgtcg gcgaaggaag gactgctggc ccaggtgggt
     1921 ggcaaccagg cgcggctgca gaagagccag ttgccggcgc tggaggaatt gctgtccaac
     1981 gacatgatgc ggcgcaagga attgctgatc cagaaaagcg tgatccgcga cattcacgcc
     2041 atgctgcacg gcagcaagaa aaccctgctg cgccggcgcg aaaatgcgct ggaagaactc
     2101 aagataatga aatccgatga aaccaatcgc aaggagcagc tggacaatct ggtgacccag
     2161 gcaaaggccg aacagggctt ctaccacaag aaactgattc tgctgaaaac cagccgcaag
     2221 atgatcgagc gcgaaggcgc gctgatgctg gaaccggtca cccaggcgcg gctggatacc
     2281 tatctgaagg aagccggcaa ggcactgacg tcgagctgga ccatcgtcgg catgaacaac
     2341 gccatcgaca ctttcttcga tcgcctgaaa gcggacatga gtgcactggc gcagaagcag
     2401 gaacagatcc agatcatggt ggcggacatc tatgcccgct acgaagcgga gcaggggctg
     2461 gcgccgctgg actatccccg tttcagcccg aaggaatacc tgacgcgcct ggacgatctg
     2521 cgcgccaagt ccggcagttt ccgccgtaac ctgcgcaagc tgctgaccga gcagaaatcc
     2581 acctcaaagc ggtttctggc gacggtggtc aacgagggct ccagcattta ccgtttgtat
     2641 gaacgcagcg gccgcgactg gctcaacaac gtgctgatgc cgctgttcca gaatgcgcag
     2701 gagcagaaac atctgctgga tgagcatgtg cagaaactgc ggctgatggc cagcgttgac
     2761 ggcaacgccg ccgagcgtgt cgggcagatg gaagccatgc tggccgatgt ggaaacccag
     2821 atcgcggaag tggaagacat gatcaagggc ctgcgcaagc cggccccgat ccatcaacaa
     2881 cgcaaggtga ttccgctgat caccgcgcag ttctcgccgc cggcgccggt gaaaaaatag
     2941 cggcgccttt tactgcggcg tgcgcatgac catgagtcgc aattccgtca tgtcgtgcat
     3001 ggcccagcgg atgccttcgc ggcccagacc gctgtccttc acgccgccat acggcatgtg
     3061 atccacccgc cacgacggca catcgccgat caccacgcca cctacttcca gctcatccca
     3121 cgccttgtgc gccttgtaca gatcgcgggt aaaaatgccg gcctgcaaac cgaagcggct
     3181 gttgttcacg tctttcagca cctgatcgaa atcggtgaat ttcgacagga tcgccagcgg
     3241 gccgaacgct tcctcggcgc aggcattctg ttgcgagtcg acattctcca gcagcgtcgc
     3301 ttccagcatg ttgcccttgc gctggccacc gcacagcagc gtggcgccgg actcgatggc
     3361 ttcgttgatc cagccgtgca gacgtttggc ttcgccttcg ctgatcatcg ggccgatgaa
     3421 ggtgtcggga tttttcgggt cgccgctttt cagctgcctg gttgcggcca ccagtttcgc
     3481 tttgagttgt tcgtaaatat ccgcgtgcgc ataaatgcgc tgcacgctga tgcagctttg
     3541 tcccgactgg tagaaagcgc cgatgatgat gcgcttcact gcgtcgtcga tgtcccagtc
     3601 tttatccacc acacaggcgg cattg
//
LOCUS       DASUEG010000216         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2061009, whole genome shotgun sequence.
ACCESSION   DASUEG010000216 DASUEG010000000
VERSION     DASUEG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061009"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFX11_04110"
     CDS             <1..1063
                     /locus_tag="VFX11_04110"
                     /inference="COORDINATES: protein motif:HMM:NF033876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellinolysin"
                     /protein_id="HEX4937834.1"
                     /translation="VGADGKTEGMMLNIDLADFNPPNLPDGGTDPFYNDRVIAHEMVH
                     AVMGRATTFNGITDWFKEGTAEFIHGADERLAGDIASVGVGGVMAAYGSIASSAGYSA
                     AYAATRYMHDRIKEAGGEGIKDIMTYLSGNAGATLDDALANASSGAFSGEADFDTDFG
                     NDGASYITNEMDLTNEDTGAIGGFDADGGEILGAEDILPNNDSAADKPLENFKLVVED
                     AYEVFNGPEFSLHIGAESMQSLTLELSAFGSWELGVHDIDLTTHPGDGVRLVDSALRY
                     IDSRRGDMGAVINRLESTISNLAAVNEALAGSRSRIRDADFAAETADLSKAQVLQQSG
                     IAILSQANAQPQAVLNLLR"
     gene            1234..3258
                     /locus_tag="VFX11_04115"
     CDS             1234..3258
                     /locus_tag="VFX11_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4937835.1"
                     /translation="MIKRTVLALAIAPLCTTALAESDGDLFGLNLTQLMNLEVVSATK
                     RPQTLADTAAAMHVISQDDIRRSGATTLPEVLRQAPGVEVARINASTWAVTVRGFQSQ
                     FANKLLVMVDGRSVYTPLFSGVYWDAEMPMLEDIDRIEVIRGPGSSVWGSNAVNGVIN
                     IITKHSADTQGQLITAGAGDVENGFVRARTGHDYGSGTVRVYGQYRNQDQSIDAATGD
                     SAQDDYQASSAGLRTDWDNGAEDRFTLQSDYQRLEKNYNIALDPYVAAQIPNFREDIG
                     SDNANALFRWQRPFANGDSQELQTYLDWTDRNESTYAYRRTTVDVDYQYNLHQISMNR
                     LTLGLNYRYITDHFEGTYAFDLDEPDLDYDQGTMFIQDEISFTDEFATILGVKLESTA
                     FTNFEYQPTARFLWTPDQDLTLWAAASRAIATPSRAARGITVNNGVKQSLQDRARTLY
                     PFAVVLGELSGQEDFESESVISYELGYRHQITPEFFVDATTYYNEYQDLRTFDPVGSG
                     YRFRITPAPSTFVYQLEYGNEAEAHSTGFEMAANWHPSDIWQLKLTYTYFNLVVEPKD
                     DHNGLGALELVDQSPQHQAMLRSQHEIGELWEFDWTVKYMDELPDDEIEAYTDMDMRL
                     GYRATPEVDLSLVGRDLFASPRTEFQDTIQGPYRTEMARSVHLQATWRPE"
     gene            3394..3846
                     /locus_tag="VFX11_04120"
     CDS             3394..3846
                     /locus_tag="VFX11_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4937836.1"
                     /translation="MGIPRTTQDIMATQVLTCTEQDTLATAHKLMRDHHIRHMPVVNS
                     QSGEFMGLVTQKEVLRQAFTIAGTQGLAALEHAEARIPVTQVMSRDTETVQPQLPLLA
                     AGQYFLESKHGCLPVVVDGKLVGIVTSADFVKLSVKLLELNASQASTQ"
     gene            3877..>3982
                     /locus_tag="VFX11_04125"
     CDS             3877..>3982
                     /locus_tag="VFX11_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004782086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4937837.1"
                     /translation="MKKVLVTGAAGFIGSHLVQQLTQKGIRVKAAVMPR"
BASE COUNT          895 a         1242 c         1088 g          757 t
ORIGIN      
        1 tgtcggcgcg gacggcaaga ccgaaggcat gatgctcaat atcgacctgg ccgatttcaa
       61 cccgcccaac cttcccgacg gcggcaccga ccctttctat aacgatcgcg tgatcgccca
      121 cgaaatggtg catgccgtca tgggccgtgc caccacattt aatggcatca ccgactggtt
      181 caaggaaggc actgcggaat tcattcacgg cgccgacgaa cgtctggcgg gcgacattgc
      241 cagtgttggc gttggcgggg tcatggccgc ctacggtagt attgccagca gcgcaggata
      301 ttctgccgcc tacgcggcca cccgttacat gcatgatcgt atcaaggagg ccggcggcga
      361 gggtatcaaa gacatcatga cctatctgtc cggcaatgca ggcgcgacac tggatgacgc
      421 acttgccaat gccagcagcg gcgccttttc cggcgaagcc gatttcgaca ctgatttcgg
      481 caacgatggc gccagctaca tcaccaacga gatggatctc acgaacgagg acacgggcgc
      541 cattggcggc ttcgatgcgg atggcggcga gattctcggg gctgaagaca ttttgccgaa
      601 caacgacagc gccgctgaca aaccactgga gaatttcaaa ctggtggtgg aagacgccta
      661 cgaagtcttc aacggaccgg agttttcgtt gcacatcggg gcggagtcca tgcaatccct
      721 gacactggaa ctgagtgcct ttggcagctg ggaactgggc gtgcacgaca tcgacctgac
      781 cacgcatccc ggtgatggtg tgcggctggt ggattcagcg ctgcgttaca tcgattcccg
      841 ccggggcgac atgggcgctg tcatcaaccg gctcgaatcc accatttcca atctggccgc
      901 ggtgaatgaa gcgctggccg gcagccgctc ccgcattcgc gatgccgact tcgccgctga
      961 aacggccgac ctgagcaagg cccaggtgtt gcagcagtcc ggtattgcca ttctgtcgca
     1021 ggccaacgcc cagccccagg ccgtcctcaa cctgctgcgc tgatcaattc tgcggcggca
     1081 tcccatttca gtttttcatt acaatctctc attacaattt cctctgccgg gcagcgctga
     1141 taaaaattgc gcactattcc atttgctgca tatactcaat gcggcatagc aaaaaacgac
     1201 ataactgttc ccccacttga atggttgtca gacatgataa aaagaaccgt gctcgcgctc
     1261 gccattgctc cactgtgcac caccgccctg gccgaatctg atggggattt gttcggcctc
     1321 aacctgaccc agctgatgaa tctcgaagtg gtgtctgcca ccaagcggcc acagaccctg
     1381 gcggataccg ctgccgccat gcacgtcatt tcccaggacg acatccgccg ctccggcgcc
     1441 accacactgc cggaagtcct gcgccaggca cctggcgtgg aagtggcacg catcaacgcc
     1501 tccacctggg cggttaccgt gcgcggtttt caaagccagt tcgccaacaa gctgctggtg
     1561 atggtggatg gccgcagcgt ttacacgccc ctcttctccg gcgtttactg ggatgcggaa
     1621 atgcccatgc tggaagacat cgaccgcatc gaagtcatcc gtggccccgg cagcagcgta
     1681 tggggttcca acgcggtgaa cggcgtgatc aacatcatca ccaaacacag tgctgacacc
     1741 cagggccagc tgatcactgc aggtgccggc gatgtggaga acggctttgt ccgtgcccga
     1801 acgggacatg actatggcag cggcaccgtg cgggtttacg gccagtaccg caatcaggat
     1861 cagtccatcg atgcagcaac cggagacagc gctcaagacg actaccaggc cagttcagcc
     1921 ggcttgcgca ccgactggga taacggcgcc gaagatcgtt ttacattgca aagcgattac
     1981 cagcgtctgg aaaagaacta caacatcgcc ctcgatccct atgttgcggc acagattccc
     2041 aattttcgcg aagacatcgg cagcgacaac gccaacgcgc tgttccgctg gcaacgcccc
     2101 ttcgccaacg gcgattccca ggaattgcag acctacttgg actggaccga ccgcaacgaa
     2161 agtacttatg cctatcgtcg cacgacggtc gatgtcgact accagtacaa cctgcaccag
     2221 ataagcatga accggctcac gctgggactt aactaccgct atatcaccga tcatttcgaa
     2281 ggcacttacg cctttgatct tgatgaacct gacctggatt acgatcaggg tactatgttc
     2341 atccaggatg aaatcagctt caccgatgag ttcgccacta tcctcggggt gaagctggaa
     2401 tccaccgcct tcaccaattt cgaatatcag cccacggccc gttttctgtg gacacctgat
     2461 caggatctga ccctgtgggc ggcagcatcg cgagcaattg caacgccatc gcgcgcggcg
     2521 cgcggcatca cggtgaacaa cggggtgaag cagtcactgc aggatcgcgc gcgtacactg
     2581 tatccgttcg ccgtggttct gggagaactg agcggccagg aggatttcga atcggagtcc
     2641 gtcatttcct acgaactggg ttacaggcac caaataacgc ctgagttttt tgtggatgcg
     2701 accacctact acaacgaata tcaggacctg cgcacatttg atcccgtcgg cagcggctac
     2761 cgtttccgca tcacgcctgc cccctccacc tttgtttacc agctcgaata cggtaacgag
     2821 gcagaagctc attccacggg gtttgagatg gcagccaact ggcacccgtc ggacatctgg
     2881 cagctcaaac tgacctacac ctatttcaat ctggtagtag aacccaaaga cgatcacaac
     2941 ggcctgggtg cgctggaact ggtcgaccag tcgccacaac atcaggccat gctgcgctcg
     3001 cagcatgaga tcggcgagct gtgggaattc gactggaccg tgaaatacat ggacgaactg
     3061 ccggacgacg agatcgaagc ctacaccgac atggacatgc gcctgggcta ccgtgccaca
     3121 ccggaagtgg atctgtccct ggtgggtcgc gacctgtttg cctcaccccg caccgaattc
     3181 caggacacca tccagggccc ctaccgcacc gagatggccc gttcggttca cctgcaggcc
     3241 acctggcgac cggaataagc cagtcctgtc tatcctgccg cgacgccagc cccacccgcg
     3301 cgtcaggatt tgccttcccc gcccacctcc gtttcagcgt ctattcttag aacgagcgcc
     3361 gccacgacgc cggcacaatg aaggagaaca accatgggca tcccccgcac cacccaggac
     3421 atcatggcca cccaggtgct gacctgcacc gaacaggaca ccctggccac cgcccacaaa
     3481 ctcatgcgcg accaccacat ccgccacatg ccggtggtga acagccagag cggcgaattc
     3541 atgggcctgg tcacccagaa ggaagtactg cggcaggctt tcaccattgc cggcacccag
     3601 gggctggccg cgctggagca cgccgaagcg cgcattcccg tcacgcaggt catgagccgt
     3661 gacaccgaaa ccgtgcagcc gcaattgcca ttgcttgcgg cgggccagta ttttctggaa
     3721 agcaaacacg gttgtctgcc cgtggtcgtg gacggcaaac tggtgggcat cgtcacctcg
     3781 gcggatttcg tcaagctcag cgtcaagctg ctggaactca atgcatcgca agcgtcaaca
     3841 caataatcga caccagaaaa acgacgggta gcagacatga aaaaggtact ggtcacaggc
     3901 gcggcaggat ttattggctc gcatctggta caacaactta cacagaaagg gatcagggtg
     3961 aaggcagcgg tgatgccccg cg
//
LOCUS       DASUEG010000217         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1181976, whole genome shotgun sequence.
ACCESSION   DASUEG010000217 DASUEG010000000
VERSION     DASUEG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181976"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX11_04130"
     CDS             <1..198
                     /locus_tag="VFX11_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX4937838.1"
                     /translation="ARQGACDADHRVFGTSGLYVMDGSAVPGSLGANPQVTIMAMATR
                     AAEKLAGTLNAEFPIEDRVAC"
     gene            192..638
                     /locus_tag="VFX11_04135"
     CDS             192..638
                     /locus_tag="VFX11_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937839.1"
                     /translation="MLTLKLHETMSGWIELNADRKKEALEFSIDVIFLNRSEPWQAQP
                     FSGVLRLSDRRYETHVQGTLSFKISGPRYELLFDHPDLGAIELKGEKSYDLFNLKESL
                     TVCPLTVYQDGNAIGYAEVAYRDSILAFPFRSLRLVKDAGLLGARG"
     gene            635..1651
                     /locus_tag="VFX11_04140"
     CDS             635..1651
                     /locus_tag="VFX11_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937840.1"
                     /translation="MNAVADPFAEQRQRLNACRFTGQTDGHYESYFLRANHPDRPLAF
                     WIRYTIFAPRKNPQNNIGELWVIVFDGERNRMTAAKSEYPLADCSFDNTGLNARIGAA
                     TLFNGHAQGEVKQRDNRIQWRLDYSPGVEPVLLLPDNLYATALPKAKALVANPLAIFR
                     GELRVNDEIMQVDGWTGSENHNWGSKHTDQYAWGQVAGFDNAPDAFLECGTARIKLGP
                     LWTPWLTTLVLRLDGRAIKLNGLLKAARANAHYGYFHWHFDTADDNVRIVGDIQAPRE
                     HFIGLNYYNPPGGSHTCLNCKIASCRLLVQEKGKPDRVLHTASRAAFEILTDDTHHGV
                     SVVA"
     gene            1855..>2130
                     /locus_tag="VFX11_04145"
     CDS             1855..>2130
                     /locus_tag="VFX11_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937841.1"
                     /translation="MLIPKLIARAVSAVCLLALVTGHSLAWADTIGIGLINPLLTPQE
                     QGWLVMENVQDPLDVSANGAFITASTLGAVVPGQGNIGVQLFHRYVPI"
BASE COUNT          435 a          630 c          629 g          436 t
ORIGIN      
        1 gcgcgtcagg gcgcctgcga cgcggaccac cgcgtgttcg gcaccagcgg tctgtacgta
       61 atggacggca gcgccgtacc cggctccctg ggcgccaatc cgcaggtgac catcatggcc
      121 atggccaccc gcgcggcgga aaaactggcc ggcaccctta atgccgaatt cccaatcgag
      181 gacagagtgg catgctgaca ctgaaactgc acgaaaccat gagcggctgg atcgaactga
      241 atgctgaccg caaaaaggaa gcgctggaat tttccatcga cgtgattttt ctcaatcgca
      301 gcgaaccctg gcaggcgcaa ccgttcagcg gcgtgttgcg gctgagcgat cgtcgctacg
      361 aaacccatgt gcaaggcacc ctgtcattca agatatccgg gccgcgctat gaattgctgt
      421 tcgatcaccc cgacctgggt gccatcgaac tcaaaggcga gaaaagctac gacctgttca
      481 atttaaaaga atcgctgacg gtgtgcccgc tcacggtcta tcaggacggc aatgctatcg
      541 gctatgcgga agtggcctat cgcgattcga tcctggcatt tccgtttcgc tcgctgcggc
      601 tggtgaagga cgccggcctg ctgggtgcgc gcggatgaac gccgtcgccg atccttttgc
      661 cgagcaacgc cagcgtctga atgcctgccg tttcaccggg cagactgatg gtcattatga
      721 aagttatttc ctgcgtgcca accatcccga ccggccgctg gcgttctgga ttcgctacac
      781 gatttttgcg ccgcgcaaaa acccgcagaa caatatcggc gagctgtggg tgatcgtgtt
      841 tgacggtgag cgcaaccgga tgacagcagc gaagtctgaa tatccgctgg ccgattgcag
      901 tttcgacaac accggcctga atgcgcgcat tggcgcagcc acgctgttta acggtcatgc
      961 acagggtgag gtcaaacagc gcgacaaccg gattcagtgg cgtctcgatt acagccccgg
     1021 tgtcgagccg gtgttgctgc tgccggacaa tctgtacgcc acggcgctgc cgaaagccaa
     1081 ggcgctggtg gccaatccgc tggcgatttt tcgtggtgag ctgcgcgtta acgacgagat
     1141 catgcaggtc gatggctgga ccggcagcga gaatcacaac tggggttcca aacacaccga
     1201 ccagtatgcc tggggtcagg tagcaggctt cgataatgcg cctgatgcat tcctggaatg
     1261 cggtaccgcc cgcatcaaac tgggcccgct gtggacgccc tggctgacga ctctggtgct
     1321 gcgcctggat ggccgcgcga tcaaactgaa tggcttgttg aaagccgccc gcgccaacgc
     1381 ccactacggt tatttccact ggcatttcga tacggcagat gacaatgtgc gcatcgtcgg
     1441 tgacatccag gcaccgcgcg aacattttat cggcctgaat tattacaatc ctcccggcgg
     1501 ttcccacacc tgcctgaact gcaagattgc cagctgccgt ctgctggtgc aggaaaaagg
     1561 caagcccgat cgtgtgctgc acactgcatc acgcgcggcg ttcgaaattc tcaccgatga
     1621 tacccatcac ggcgtctccg tcgtggcatg accggacgca cttgcatgtg ccgggcaatc
     1681 ggcggacgat aaccggtgct tccggcaata ccggattcag gctggatgct gtcattccgg
     1741 caggcgcggg aaaaatgaca atattttcct gcgcttgcct gttcccaccg gagcgacaga
     1801 ttgttgaacg gtgccgcccc ggacatcgtg acaactacgc acaggagagt gcatatgctt
     1861 attcccaaat tgatcgcgcg cgctgtttcc gccgtttgcc tgcttgcgct ggtaaccgga
     1921 cattcgctgg cctgggccga cactattggc atcggtttga tcaatcccct gctgacgccg
     1981 caggaacagg gctggctggt gatggaaaat gtgcaggacc cgctggatgt cagcgccaac
     2041 ggcgcgttca ttacggccag caccttgggc gcggtggttc ccgggcaggg caatattggc
     2101 gtgcagctgt ttcaccgcta tgtgccgatc
//
LOCUS       DASUEG010000218         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1087746, whole genome shotgun sequence.
ACCESSION   DASUEG010000218 DASUEG010000000
VERSION     DASUEG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087746"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VFX11_04150"
     CDS             complement(<1..156)
                     /locus_tag="VFX11_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX4937842.1"
                     /translation="MRMRTLELTVGAFMLAGVLALVFLALRVSGLSAESGEDTYRIKA
                     KFENIGGL"
     gene            complement(158..940)
                     /gene="mlaE"
                     /locus_tag="VFX11_04155"
     CDS             complement(158..940)
                     /gene="mlaE"
                     /locus_tag="VFX11_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX4937843.1"
                     /translation="MIDRIRLMGRNGLDVLNAFGRSGLFIFHSLWALPNLRTNVPLTV
                     KQIYSVGVLSLVIIVVSGLFIGMVLALQAYNILNRFGSEQALGQMVALSLLRELGPVV
                     AALLFAGRAGSALTAEIGLMKATEQLASMEMIGVDPLRRIIAPRFWAGVICMPTLALI
                     FSAVGIWGGYLVGVPWLGVDDGSFWANMQESVEWGEDVFNGIIKSIVFGIVVTWIAVF
                     QGYDTIPTSEGISRSTTRTVVYASLAVLGLDFLLTAVMFGDL"
     gene            complement(937..1752)
                     /locus_tag="VFX11_04160"
     CDS             complement(937..1752)
                     /locus_tag="VFX11_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4937844.1"
                     /translation="MSPNPENYVEIRNMSFYRGERAIYNNVNIRIRKGKVTAIMGPSG
                     TGKTTLLRLIGGQLKPDQGEIFVDGHNIPRLNRKELFQVRSKMGMLFQSGALFTDISV
                     FENVAFPLRVHTRLPEDMIRDLVLMKLQAVGLRGAKDLMPSELSGGMARRAALARAIA
                     LDPELVMYDEPFAGQDPMAMGVVVQLVRLLNDALGHTSIVVSHDVHETASIADYIYVI
                     ADGEVIGEGTSEEVMNSSLPRVRQFMQGLPDGPVPFHYPARKLGDDFLRGNPA"
     gene            2059..>2974
                     /locus_tag="VFX11_04165"
     CDS             2059..>2974
                     /locus_tag="VFX11_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX4937845.1"
                     /translation="MEFDFIRSGQRVVDIEAAATQALHARIDATFARACQIILACKGR
                     VIVTGMGKSGHIGTKIAATLASTGTPAFFVHPGEASHGDLGMITGTDVVIAISNSGKT
                     EEIVTIVPLIKRKGAPLISMTGVPTSPLATQSDVNLDVAVKEEACPLGLAPTASTTAT
                     LVMGDALAVALLEARGFTSDDFALSHPGGSLGRRLLLLVENIMRTGNDIPLVRDDTTV
                     SHALLEMSSKGLGMTGVIDHSGKLSGIFTDGDLRRALDHGTDLKNTPIRDVMTRNPKT
                     VQAHVLAVEALNLMDQKKITALMVLDRDG"
BASE COUNT          621 a          989 c          857 g          507 t
ORIGIN      
        1 gaggccgccg atattctcga atttggcctt gatgcggtag gtgtcctcgc cgctctcggc
       61 gctcaggccg ctcacccgca gcgccaggaa caccagcgcc agcacacccg ccagcatgaa
      121 agcgccgacg gtcagttcca gggtacgcat gcgcatctta cagatctcca aacatgactg
      181 cggttaaaag gaagtccagg cccagcacgg cgagggacgc gtacaccacg gtacgggtcg
      241 tggaacggct gatgccttca gaagtgggaa tggtgtcgta accctgaaac acggcgatcc
      301 aggtgacgac aataccgaat acgatgctct tgatgatgcc attgaacaca tcctcgcccc
      361 attcgacgct ttcctgcatg ttggcccaga acgaaccatc gtccacgccc agccagggta
      421 cgcccaccag gtagccgccc cagatgccga cggcagaaaa gatcagcgcc agcgtcggca
      481 tgcagatgac accggcccag aaacgcggtg caatgatgcg gcgcagcggg tccaccccga
      541 tcatttccat gctggccagc tgttcggtgg ctttcatcag gccgatctcg gccgtgaggg
      601 cggaaccggc gcgacccgca aacagcagcg ccgccaccac cggacccagt tcccgcagca
      661 ggctcagcgc caccatctgc cccagggcct gttcactgcc aaagcggttc aggatgttgt
      721 aggcctgcaa cgccagcacc atgccgataa acaggccgga caccacgatg atcaccagcg
      781 acagcacacc caccgaatag atctgcttca cggtcagggg aacattggtg cgcagattgg
      841 gcaacgccca cagggaatgg aaaataaaca ggccggaacg gccgaaggca ttgagcacat
      901 cgagcccatt gcggcccatc aggcggatgc ggtcgatcat gccggattcc cccgcaggaa
      961 atcgtcaccc agcttgcgcg ccggatagtg gaacggcacc gggccgtccg gcaatccctg
     1021 catgaactgg cgcacccggg gcaggctgga attcatcact tcctcggagg tgccctcgcc
     1081 gatcacctcg ccatcggcga tcacatagat atagtcggca atgctcgccg tttcgtgcac
     1141 gtcatgggac accacgatac tggtatgccc cagcgcgtcg ttgagcagcc gcaccagctg
     1201 caccaccacg cccatggcca tcgggtcctg gccggcaaag ggttcgtcgt acatcaccag
     1261 ttccgggtcc agcgcgatgg cccgcgccag tgccgcccgc cgcgccatgc caccggacag
     1321 ttcgctcggc atcagatcct tggcgccacg cagccccacg gcctgcagct tcatcagcac
     1381 cagatcgcgg atcatgtcct ccggcagccg ggtgtgaacc cgcagcggga aggccacatt
     1441 ctcgaacacg gaaatatcgg taaacagcgc gccgctctgg aacagcatgc ccatcttgct
     1501 gcgcacctgg aacagttcct tgcgattcag ccggggaatg ttgtggccat ccacaaaaat
     1561 ctcgccctgg tcaggcttga gctggccacc gatcagccgc agcagcgtgg tcttgccggt
     1621 gccgctggga cccatgatcg cggtgacctt gcccttgcgg atacggatgt tgacgttgtt
     1681 gtagatcgcc cgctcgcccc ggtaaaagga catattgcgg atctcaacat agttttcggg
     1741 attgggtgac atgaacgtcc tatcgactgg cgaatgcacg cgcaccggct gcagcgggat
     1801 atggccccgg ccatgcagga taagggccgt acactttata caagggccgc actatagcat
     1861 tccggccggt aaaggaaaaa tccgtggtca cacttgcagg gtcgacggtt tctcattaaa
     1921 ttgctgccgc ttttgtcgcg cagcatttgc cgcgtaactt tagtcgtaca ggggggcagg
     1981 gaacggatct gacagttcgc gcccgattgg tttacactgg gcgccaccgt gaccgcagcc
     2041 cccgaggaca cgcacaccat ggaattcgat ttcatccgct ccggccagcg cgtcgtcgat
     2101 atcgaggcag cggccaccca ggccctgcac gcccgcatcg acgccacgtt tgcccgcgcc
     2161 tgccagatca tcctggcctg caagggtcgc gtcatcgtca ccggcatggg caagtccggt
     2221 catatcggca ccaagatcgc cgccaccctg gccagcaccg gcaccccggc tttttttgtc
     2281 catccgggcg aagccagcca cggcgacctg ggcatgatta ccggcaccga cgtggtgatc
     2341 gccatctcca actccggcaa gaccgaggaa atcgtcacca tcgtgcccct gatcaagcgc
     2401 aagggggccc ccctgatcag catgaccggt gtccccacct cgccgctggc cacccagtcc
     2461 gacgtgaatc tggatgtggc cgtaaaggaa gaagcctgtc cgctcggact ggcccccact
     2521 gccagcacca ccgccacgct ggtgatgggc gacgcactgg cagtcgcact gctggaagcc
     2581 cgcggtttca cctcggacga tttcgcgttg tcgcatcccg gtggcagtct tggccgccgc
     2641 ctgctgctgc tggtggaaaa catcatgcgc accggcaacg acattcccct ggtcagggat
     2701 gacaccaccg tcagccacgc cctgctggaa atgagcagca agggcctggg catgaccggc
     2761 gtgattgatc acagcggcaa actgtctggc atcttcaccg acggcgacct gcgtcgcgcg
     2821 ctggatcatg gcaccgacct gaaaaacacc ccgatccgtg atgtgatgac cagaaatccg
     2881 aaaaccgtgc aggcccatgt gctggccgtg gaagcgctga acctcatgga ccagaaaaaa
     2941 atcaccgccc tgatggtgct ggaccgcgac ggca
//
LOCUS       DASUEG010000219         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2008089, whole genome shotgun sequence.
ACCESSION   DASUEG010000219 DASUEG010000000
VERSION     DASUEG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008089"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFX11_04170"
     CDS             <1..526
                     /locus_tag="VFX11_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX4937846.1"
                     /translation="PDKFEVREFFWFGCGHCFQLEAPLEEWAKQAPADVNFVRTPAPM
                     NDRWVSHAHAFYVAESLGKLDQITPALFNALHVKKESIHSQEELATFFTGFGISEDDF
                     NKRYNSFSVRTKVRQARTQAQQYGLRGVPALVVNGKYLVKGKEGAGPAYMLRVVDFLL
                     DRERVARQITQPVK"
     gene            611..1444
                     /locus_tag="VFX11_04175"
     CDS             611..1444
                     /locus_tag="VFX11_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4937847.1"
                     /translation="MTFKINSLPAAQPAAAAPPQVLRLLSYNIQAAIGTSSFHHYVTR
                     SWRHVLPDWRGLQRLDRIAQVLAHYDLVSLQEVDGGSLRSGFVNQVAYLADLARFEYW
                     FQQLNRNLGKLGQYSNGVLSRFVPYEIEDHKLPGLKGRGAIVAKYGNAENTLVLVNLH
                     LALSEKARFRQLGYVRELIQHHEHVVVMGDMNCRSDQIIDTPLRDTHLLQVTGNHHTY
                     PSWNPTRNIDHILVSPSLTINHVKVLDYDYSDHRPIAMEVQLPQPLADAMGTQGAGSR
                     P"
     gene            1548..>2875
                     /locus_tag="VFX11_04180"
     CDS             1548..>2875
                     /locus_tag="VFX11_04180"
                     /inference="COORDINATES: protein motif:HMM:NF044950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4937848.1"
                     /translation="MAETGVQNWKEKYLAALEEFETSHKKDQQRLDLLRRGLVRVSLA
                     ADGLDKTLDQQLDELRTGLRGSRDINSLEPLVSQLERTIIALDDRRKDNHGELESLLD
                     AQLEQCLTLDLPRQEKSAVKKLRKELPDLLANSGASLELWKQFQQVQEQVRQHLALRI
                     DELSANRGSFLSRLFSGHQHPATDPAPDVTVSHVSREPDRHLPSRSDEYQLEEPGLTG
                     TVVPENEPEADPHLLERLSRLLCNLLSQLEVPVEFSVRKEKLVDRIKSPFTLEQLPEF
                     IDETTQLVASTRLTAQKEFESFLVALHQRLLDIQEFLVTAKQGEEKSLRNQEKLDADV
                     RQELKEMRVSVAGTEDMGRLRQEIEGMVNRIVAAVDSFHLEEKSRRDEVFGRIEILAK
                     RMESMEAEASELKSSLEAQRMEALRDALTELPNRAAYDEQIEREFSRWRR"
BASE COUNT          632 a          876 c          862 g          505 t
ORIGIN      
        1 gccggacaaa ttcgaggtac gcgagttctt ctggtttggt tgtggccact gcttccagct
       61 ggaagccccg ctggaggaat gggcgaagca ggcaccggcc gacgtgaatt tcgtgcgcac
      121 accggccccc atgaacgatc gctgggtcag ccacgcccat gcgttctacg tggcggagtc
      181 cctgggcaag ctggaccaga tcacaccggc cctgttcaat gccctgcacg tgaagaaaga
      241 gagcatccat agccaggaag aactggccac gttcttcacc ggcttcggca tcagcgaaga
      301 tgacttcaac aagcgttaca attccttcag cgtgcgtacc aaggtacgtc aggcccgtac
      361 ccaagcccag caatatggtc tgcgcggcgt gccggcgctg gtcgtaaacg gcaagtacct
      421 ggtaaagggc aaggaagggg ccggcccggc ctatatgctg cgggtagtgg actttctgct
      481 ggaccgggaa cgtgtggccc ggcaaatcac ccagcccgtc aaataaaacc ctggcgggcg
      541 ccagaaactt tcacacgcaa cacctacact gaaaccctgg cttgaccgtt tgcattcggg
      601 tcgcactacc gtgacattca agatcaactc cttgcctgca gcccagcctg ccgcagccgc
      661 tccaccccag gtgttgcggc tgctgagcta taacatccag gccgccattg gcaccagctc
      721 gtttcaccac tacgtgacgc gcagctggcg ccatgtgctg ccggactggc gtggcctgca
      781 acggctggac cgcatcgccc aggtgctggc tcactatgat ctggtgtcgc tgcaggaagt
      841 ggacgggggc agcctgcgca gcggcttcgt gaatcaggtg gcctacctgg cggacctggc
      901 ccggtttgaa tactggtttc agcagctgaa ccgtaacctg ggcaaactgg ggcagtacag
      961 caatggcgtg ctgtcgcgtt tcgtacctta cgaaatcgag gatcacaagc tgccgggcct
     1021 gaaggggcgc ggcgccatcg ttgccaaata cggcaatgcc gagaacacgc tggtgctggt
     1081 gaaccttcac ctggccctga gcgaaaaggc ccgtttccgc cagctgggtt atgtgcgcga
     1141 gctgatccag caccacgagc acgtggtggt gatgggcgac atgaactgtc gcagcgacca
     1201 gatcatcgac accccgctgc gggataccca cctgctgcag gtgaccggca accatcacac
     1261 ctaccccagc tggaatccca cccgcaacat cgaccacatc ctggtcagcc ccagtctgac
     1321 aatcaatcat gtaaaagtgc tggattacga ctactccgat caccgcccca ttgccatgga
     1381 agtgcaattg ccacaaccac ttgccgacgc gatgggcacg cagggcgccg ggtcccgccc
     1441 ctgacctgga tatccccaca gcctgtgtaa tctttccggc gtgacggctg aagttttcta
     1501 tactttgtct catcacccca aagatttgct gcagggcctg catcgacatg gcggaaaccg
     1561 gtgtccagaa ctggaaggaa aaatacctgg ccgcgctgga ggagtttgaa accagccaca
     1621 agaaggacca gcagcggctg gatctgctgc ggcggggtct ggtccgggtc agtcttgcgg
     1681 cggatgggct cgacaaaacc ctggaccagc aactggacga gctgcgcacc ggactgcggg
     1741 gcagccgcga catcaattcc ctggaaccgc tggtgtccca gctggaacgc accatcatcg
     1801 cactggacga ccgccgcaag gacaatcacg gcgaactgga atccctgctg gatgcgcagc
     1861 tggaacagtg tctgaccctg gatctgccac ggcaggaaaa atccgccgtc aaaaaactgc
     1921 gcaaggaatt gccggacctg ctggcgaaca gcggcgccag cctggaactg tggaagcagt
     1981 tccagcaggt gcaggaacag gtacgacagc atctggcgct gcgcatcgac gagctgagcg
     2041 ccaatcgcgg cagctttctt tcgcgtcttt tttccggaca ccaacatccg gccaccgatc
     2101 ccgcgccgga cgtgaccgtg agccacgtca gccgtgagcc ggaccgtcat ttgccctcac
     2161 gctctgatga ataccagctg gaagaacccg gtcttaccgg caccgttgtg ccggagaatg
     2221 agccagaggc cgatccccac ctgcttgaac gcctcagccg tctgttgtgc aatctgctca
     2281 gccagctgga agtgcccgtg gaattcagcg tgcgcaagga aaagctggtg gaccgcatca
     2341 agtcgccgtt cacgctggag cagctgccgg aattcatcga cgagaccacc cagctggtgg
     2401 cctccacccg cctgaccgcg cagaaggagt tcgagagttt tctggtggcg ctgcaccagc
     2461 gcctgctgga tatccaggag ttcctggtga ccgccaagca gggcgaagaa aaatccctgc
     2521 gcaaccagga gaaacttgac gccgatgtgc gccaggaatt gaaggaaatg cgggtgagcg
     2581 tggccggcac tgaggatatg ggccgtctgc ggcaagaaat cgaaggcatg gtaaaccgca
     2641 tcgtggcggc ggtggacagt tttcacctgg aggaaaaaag ccgccgcgac gaggtgttcg
     2701 gccgcatcga aatattggcc aaacgcatgg agtcaatgga ggcggaagcg tcggaactca
     2761 agagcagcct ggaagcacaa cggatggaag cgctgcgcga cgcgctgaca gaattaccca
     2821 atcgtgccgc ctacgatgaa cagatcgagc gggagttttc gcgctggcgc cggca
//
LOCUS       DASUEG010000220         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1286638, whole genome shotgun sequence.
ACCESSION   DASUEG010000220 DASUEG010000000
VERSION     DASUEG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286638"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1923
                     /locus_tag="VFX11_04185"
     CDS             142..1923
                     /locus_tag="VFX11_04185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4937849.1"
                     /translation="MSCLLLLLCGAAGAAESLVLEARAGRVSLDSHLAYLEDPTLQLT
                     LDQIRAPDRQWTDNGNDVLNKSYSDSAWWVHLQLVNPLPDEQSRLIEISYAVLDYIDL
                     YVVSGDQVVQQYKMGDKLPYRERLFDHRFFVIPLDWQPRQTLDIYLRVRTSSSVQVPL
                     TLWQSDEFFGFDTTRTILQGIFFGTMLVIAVYNLLIYFVLGERTYILYVGYVLCMPAF
                     LASLGGHTFRYFWPTLTEWNDESIIVFLAGVVVFAATFTRRFLDLRNFNRGLDIALIV
                     TAVAGGVMVLTAFMLPYRYNIRTLIPLAAAGCIICFIAGIYCWYRHQETAKYYTIAWG
                     CMLSGGLILALNKYDLIPANIFTEYATHIGSSLEVVLLSFALAERINAERRLRFEAQA
                     QALKTTQRMNEELEGRVHERTRELEALTLRLRELSNTDALTGLHNRRFLDNQLREEWE
                     RCRRYQRSLAVILLDIDHFKQVNDLHGHQSGDECLFEVAQRVQQGLRWPADQAARYGG
                     EEFCIVLPETALEGAVTVAERIRQLIEDLPVDTTTGPLRITVSAGVCALIPDTGTSIG
                     QLLKNADQALYKAKEAGRNRVLTHLEN"
     gene            2073..>2723
                     /locus_tag="VFX11_04190"
     CDS             2073..>2723
                     /locus_tag="VFX11_04190"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX4937850.1"
                     /translation="MQAFTFTTTKTILNEAGSATRLGELCKDQGARSVLIVTDPGIRK
                     VGLLDKVLPGFASAGVKVAVFDETEADPSDVTVLKAVDLGKAHKVDLVVGFGGGSSMD
                     VAKLVAFLAAPKCTQSLPDMYGVNNAKGARLPLIQVPTTAGTGSEVTPIAIVTTGATT
                     KSGVVSSVLQPDIALLDAELTLGLPPQVTAATGIDAMVHAIEAYTSRHKKNPVSDML"
BASE COUNT          509 a          839 c          811 g          564 t
ORIGIN      
        1 ccctgcgccg gtggggacgg gccggagtat ctgtcgggcg gacaattccc gcctgctctt
       61 cactttgctg ctaagctgaa tttattccca cttttcactg cataggcgga ttccgttccc
      121 gtgattttgc tgcggtcctg cttgtcctgt ctgctcctgc tgctgtgtgg cgccgccggc
      181 gccgcggaat ccctcgtact ggaagcgcgg gccggtcgcg tcagtctgga cagtcatttg
      241 gcctacctgg aagatcccac cctgcagctg accctggatc agatccgggc accggaccgg
      301 cagtggactg acaatggcaa tgatgtactc aacaagagtt acagcgattc tgcctggtgg
      361 gtgcacctgc aactggtcaa ccctctgccg gacgagcagt cacgcctgat cgaaatcagt
      421 tacgccgtac tggactatat cgacctgtat gtggtgagcg gcgatcaggt cgtgcagcaa
      481 tacaaaatgg gcgacaagct gccctatcgc gagcgtctgt tcgaccaccg ctttttcgtg
      541 attcccctcg actggcaacc ccggcagacg ctggatattt acctgcgtgt gcgcaccagc
      601 agctcagtac aggtaccgct gaccctgtgg cagagcgacg agtttttcgg tttcgatacc
      661 acccgcacca tcctgcaggg catcttcttt ggcaccatgc tggtgatcgc ggtgtacaac
      721 ctgctgatct attttgtgct gggtgagcgc acctacattt tgtacgtggg ctacgtgctg
      781 tgcatgccgg cgtttctggc cagtctcggc gggcatacct tccgctattt ctggcccacc
      841 ctgacagagt ggaacgacga gtccattatc gtgtttctgg cgggggtcgt ggttttcgcc
      901 gcgacgttca cccgccgctt tctggatttg cgcaacttca accgcgggct ggacattgcg
      961 ctgatcgtca cggccgtggc cggcggggtg atggtgctga ccgccttcat gctgccctac
     1021 cgctataaca tccgcaccct gattcccctg gcggccgcag gctgcatcat ctgtttcatc
     1081 gccggcatct actgctggta ccggcaccag gaaaccgcca agtactacac catcgcatgg
     1141 ggctgcatgc tgtcgggcgg gctgatcctg gcgctgaaca aatacgatct gattccggcc
     1201 aatattttca ccgagtatgc tacccatatc ggttcctcgc tggaggtggt gctgctgtca
     1261 ttcgcactgg cggaacgcat caacgccgag cgccggctgc ggttcgaagc gcaggcccag
     1321 gcgctgaaaa ccacccagcg catgaacgag gaactggagg gccgcgtgca tgaacgcacc
     1381 cgcgaactgg aagcgctgac tctgcgcctg cgggaactga gcaacaccga cgcgctgacc
     1441 gggctgcaca accgccgctt cctcgacaac cagttgcggg aggaatggga gcgctgccgg
     1501 cgctaccagc gcagcctggc cgtcattctg ctggacatcg atcatttcaa gcaggtgaac
     1561 gacctgcacg gacaccagtc tggcgatgaa tgcctgttcg aggtggcgca acgggtccag
     1621 cagggcctgc gctggccggc ggatcaggct gcccgttatg gtggagagga attctgcatt
     1681 gtgctgccgg aaaccgctct ggaaggtgct gtcaccgtgg ccgagcgcat tcgccagctg
     1741 atcgaggatc tgccggtgga tacgaccacc ggaccgctgc gcatcacggt cagcgcaggc
     1801 gtgtgcgcgc tgataccgga tacgggcacg tcgatcgggc aactgctgaa aaatgccgat
     1861 caggccctgt acaaagccaa ggaagcaggc cgtaaccgcg tgctgactca cctggaaaat
     1921 tagccgttcc tggccgctta tagcgtttcc cttggccttt atgccgcagc cgtccatgct
     1981 atggtgcgct gccttgcgtt tcccacggtt acgggcagaa tggttgcaaa ggatttcaac
     2041 cgcaggacag aaccacacga ggtggaaaca ttatgcaggc tttcactttt acaaccacca
     2101 aaaccattct gaatgaagcc ggcagcgcaa cgcggctggg cgaattgtgc aaagaccagg
     2161 gcgctcgttc cgtcctgatc gtaaccgatc ccggtattcg caaggtcggg ctgctggata
     2221 aagtgttgcc aggcttcgcc agcgccggcg tcaaggtggc cgtgttcgat gaaaccgagg
     2281 ccgacccgtc cgatgtcacc gtgctgaagg cggtggacct gggcaaggcc cacaaggtgg
     2341 atctggtggt cggattcggc ggcggcagct ccatggacgt ggccaaactg gtggcttttc
     2401 tggctgcgcc caagtgcacc cagtcactgc ccgacatgta cggcgtcaac aatgcgaaag
     2461 gcgctcgttt gccgctgatc caggtgccca ccacggccgg caccggttcc gaagtcaccc
     2521 ccattgccat tgtcaccacc ggcgctacca ccaagtccgg cgtggtgtcc tcggtgctgc
     2581 agccggacat tgcgctgctg gatgcggaac tcaccctggg cctgccaccc caggtcactg
     2641 ctgccaccgg catcgacgcc atggtgcacg ccatcgaggc ttacaccagc aggcacaaga
     2701 aaaatcctgt gtcggacatg ctg
//
LOCUS       DASUEG010000221         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_336504, whole genome shotgun sequence.
ACCESSION   DASUEG010000221 DASUEG010000000
VERSION     DASUEG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336504"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VFX11_04195"
     CDS             <1..1040
                     /locus_tag="VFX11_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011575689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4937851.1"
                     /translation="GGAYVPAMSDESIIVKKQGTIFLAGPPLVKAATGEEVTAEDLGG
                     ADVHCRISGVADHFAENDDHALQLARQSVARLNRVKRIEMDRAEISAPLYPAEELLGL
                     IPTDSKKTFDVREVIARVVDGSEFDEFKALYGTTLVCGFARLYGYPVGIVANNGILFS
                     ESAMKGAHFIELCAQRKIPLLFLQNITGFMVGKEYENQGIAKHGAKMVTAVATAQVPK
                     LTLIIGGSFGAGNYGMCGRAYDPRFLFMWPNARISVMGGEQAAGVLAQVKRDNLEKAG
                     KTWSAEDELAFKRPIIDTYEHQGNPFYASARLWDDGVIDPAQTREVLGLALSATLNAP
                     IEETRFGVFRM"
     gene            1048..1836
                     /locus_tag="VFX11_04200"
     CDS             1048..1836
                     /locus_tag="VFX11_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4937852.1"
                     /translation="MHYNTIQLGIDPRGVATLALNRPDTNNAFNAEMIREMTDAITGC
                     ATDTAVRALILRSTGRHFSAGADLHWMRSTADMSVAENRADAAKLSELMRSLHEFPAP
                     TIARITGASYGGALGLIACCDMAFASDDATFCLSEVRLGLVPAVISPYVVSAIGERAA
                     RRYFLTGEVIDASLADKLGLIYRAGSPDALDETIQNICKSVLKNGPVATQEAKRLLRQ
                     VAGHPINDELRDLTIDLIARLRVSPEGQEGLNSFLEKRKPDWSK"
     gene            1833..>3243
                     /locus_tag="VFX11_04205"
     CDS             1833..>3243
                     /locus_tag="VFX11_04205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX4937853.1"
                     /translation="MITMKARYFSKVLIANRGEIACRIIRTLNRMGIRSVAVYSDADR
                     NALHVEMADEAIHIGGPSSSESYLNIPRILAAAQQCNADAIHPGYGFLSENADFAEQC
                     EAKGIRFIGPSATAIRAMGSKSAAKRLMESIGVPLIPGYHGDDQSEARLVLEAEKIGF
                     PVLLKAAMGGGGKGMRVVHAAEDLPHSIASCRREALASFSDDHLLIEKYIECPRHVEV
                     QIFCDEHGNAVHLFERDCSIQRRHQKIIEECPAPDLPEHIRTGLHRDAIAAAKSIQYT
                     GAGTVEFLYDGRAHYYFMEMNTRLQVEHPVTEMITGQDLVEWQILVAQSLPLPLRQDE
                     IVQNGHSIEVRICAENPANDFMPSIGQFQILCKPERSSNIRLDSGVRDADTISIYYDS
                     MIAKLITWDKTRDGAIRRMRQALEHFCVCGIETNISYLYQLVSNADFQQCKLATNFIA
                     VNQHTLQLTAPLSDQHVAVA"
BASE COUNT          749 a          937 c          892 g          665 t
ORIGIN      
        1 caggcggcgc ctacgtgccc gcgatgagcg atgaaagcat tatcgtgaag aaacagggca
       61 ccatttttct ggcaggaccg ccactggtga aagccgcgac aggcgaggaa gtgactgcgg
      121 aagacctggg tggcgccgat gtacattgcc gcatttccgg cgtcgccgac cactttgccg
      181 agaacgacga tcacgcactg cagctggcgc gtcagtcagt ggcacgcctt aaccgcgtca
      241 aaagaatcga gatggatcgc gccgaaataa gcgccccgct ctacccggcg gaagaactgc
      301 tgggcctgat tcccactgac agcaagaaaa ccttcgacgt gcgggaagtt atcgcccggg
      361 tcgtggacgg atccgagttc gacgaattca aagcactgta tggaacgaca ctggtgtgcg
      421 gttttgcccg cctgtacggt tatccggtcg gtatcgtagc gaataatgga attctgtttt
      481 ccgaatcggc aatgaagggc gctcacttca tcgaactctg tgcccaacgg aaaataccgc
      541 tgctgtttct gcagaacatc acgggattca tggtgggcaa ggaatacgaa aaccagggta
      601 tcgccaaaca cggtgccaag atggtgacgg cggtggccac ggcgcaggtg cccaagctga
      661 cccttatcat cggcggcagt ttcggtgccg gcaactacgg catgtgtggc cgcgcctacg
      721 atcctcgctt cctgttcatg tggcccaacg cgcgcatttc cgtgatgggc ggtgaacagg
      781 ccgccggcgt tcttgcgcag gtgaaacggg acaatctgga aaaggccggc aagacctggt
      841 cggccgagga cgaactggcc ttcaagcgcc ccatcattga tacctatgaa caccagggca
      901 atccctttta cgccagcgca cgcctgtggg atgacggcgt catcgatcca gcacagaccc
      961 gcgaagtgct ggggctggcg ttgtcggcaa cgctgaatgc accgatcgaa gagacacgct
     1021 ttggcgtatt cagaatgtga ggtgcccatg cactacaaca ccattcagct cggtatcgac
     1081 cctcgcggcg tggcgacact ggcactgaac cgcccggaca ccaacaatgc gttcaacgca
     1141 gaaatgatcc gggaaatgac ggatgccatc acaggatgtg caactgatac cgccgtgcgt
     1201 gcattgatac tgcggtcgac cggacgtcac ttctcggcgg gggccgatct gcactggatg
     1261 cgcagcacgg ccgacatgag cgttgccgag aacagggctg atgcggcaaa actgtcagag
     1321 ctcatgcgca gtttgcatga atttcccgca cccacaattg cacgaatcac aggcgcatcc
     1381 tacggcggag cactgggcct gatcgcctgt tgcgatatgg ctttcgcgtc cgatgacgcc
     1441 actttctgcc tgagcgaagt ccggcttgga ctggtgccgg ccgtcatcag cccctacgtc
     1501 gtcagcgcca tcggggaacg tgcagcccgg cgctactttc ttaccggtga agtcattgat
     1561 gcctcccttg cggacaagct tggcttgatt taccgcgccg gcagccccga tgcactggat
     1621 gaaacgatcc agaacatctg caaatccgtg ctgaaaaatg gccctgtcgc cacacaggaa
     1681 gccaaacgac ttctcaggca ggtggcagga catcccataa acgacgaatt acgcgaccta
     1741 accattgacc tgattgcccg tctccgtgtt tcaccggaag gacaggaagg gctgaattcg
     1801 tttctggaaa aaagaaaacc ggattggagc aaatgatcac tatgaaagcc cgttattttt
     1861 ccaaggttct gattgccaat cgcggtgaaa tcgcctgccg cattatccgc acactcaacc
     1921 ggatgggcat tcgcagtgtc gcggtgtatt ccgatgctga tcgcaatgct ttgcatgtag
     1981 aaatggcgga tgaagccatt cacattggag ggccctcctc cagcgaaagc tatctcaata
     2041 tcccccgcat tcttgctgcc gctcagcaat gcaatgccga cgccattcat ccaggttatg
     2101 gatttttgtc ggaaaatgcc gactttgccg aacagtgcga agccaagggc atccgcttca
     2161 ttgggccctc cgccacagcc atccgggcaa tgggatcaaa gtccgcagcc aagcggctga
     2221 tggagtccat cggtgtcccg cttattccgg gatatcacgg cgacgaccag tcggaagcga
     2281 gactggtcct ggaagcggaa aaaatcggct ttcctgtgtt actcaaggcc gcaatgggtg
     2341 gcggcggcaa gggaatgcgc gtagttcatg cagcagaaga tctgccacac tccatcgcca
     2401 gctgccgtcg tgaggcattg gcctcttttt ccgacgatca cttgctgatc gaaaaataca
     2461 tcgaatgtcc tcgccatgtg gaggtgcaga ttttctgtga cgagcatggc aatgctgtcc
     2521 acttgtttga gcgagactgc tccattcagc gccgacacca gaagataatt gaggaatgcc
     2581 ccgctcctga tctcccggaa catattcgca ccggtctgca ccgggacgcg atagcagcgg
     2641 caaagtccat acagtacacc ggcgccggga ctgtggagtt cctctacgac ggccgtgctc
     2701 actactactt catggaaatg aatacccgtc ttcaggtcga gcatcctgtt acagaaatga
     2761 tcaccggaca ggacctcgtg gagtggcaga ttctggtggc gcaatcgctt ccgctaccac
     2821 ttcgtcaaga cgaaattgtc cagaacgggc attcaatcga ggtgcgcatc tgcgctgaaa
     2881 atccggcaaa cgatttcatg ccctccatag ggcagttcca gatactgtgc aagccagagc
     2941 gttcctcgaa tatccggctt gactcaggtg tccgcgacgc agacaccatc agcatttact
     3001 acgattccat gatcgcaaaa ctgatcacct gggacaaaac ccgtgacggg gcaattcgcc
     3061 gcatgcgcca ggcactggaa catttttgcg tttgcggcat cgagaccaac atcagctatc
     3121 tctaccagct cgtttccaac gctgacttcc agcaatgcaa gcttgccacc aatttcatcg
     3181 cagtcaatca gcataccctt cagttaacag cccctctgtc ggaccagcac gtggcagttg
     3241 ctg
//
LOCUS       DASUEG010000222         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1414652, whole genome shotgun sequence.
ACCESSION   DASUEG010000222 DASUEG010000000
VERSION     DASUEG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414652"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /locus_tag="VFX11_04210"
     CDS             complement(<1..2458)
                     /locus_tag="VFX11_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4937854.1"
                     /translation="MERRRFGHWLAPTIQNKLLAVMLVVSLAPLVVFGTLAYFKSRET
                     IINQVGERLQAASLLAMSQVDRTFGFSEENIRSWAALAAMQEVERGDPQGIVSDMLTD
                     YQRAYAVYSTLVAVDLEGVVVAAGDRDLLGIQVSDAEWFQQVKATGKAQMSPLRLDPL
                     LGGYGVSIAVPIFRSFSRDEVIGVLKASFDWRELLQQIHAIEVIPGGQNEQGYAVLID
                     RDGFIIAAPDFILSSDDGSISESDLLRVYGRRWWVTENPLLLDRLLHRSGHRYIRRGD
                     TELLLVNMPAEEFRNIKDTGWSLVLVRDATDALADIAFIRERAILIGLLAILLIGVVA
                     YVMSRQIGEPIARLSRWASDLSRGNLDRKLALRSNDELGQLAVSLDNMRQDLKSYLDE
                     ITESKERYQTIMSSIDCVVWEAQIDPPRVTLLSGQVEHVLGHSPDQVLEMLGQWQRWI
                     HPDHHARIMAAFSEAVSGGRDSYVEFKVRHGDGQWVWLKALVSVVIDGLRVVGLRGVL
                     VDINDIVKASEEMADARDIAVKTAENKSRFMAIVSHEIRTPMNGMLGMLDLLNDSELN
                     AEQREALALATRSGRNLLALVDDVMDFSRLESGEMEFRHEQVNVHELFHSAVNLVAPD
                     AYRAGLDIGVVLEANLPQKIVTDTTKLRQLLTSLLSNAVKFTSHGSILLWAEMLPPHR
                     LYVEVKDTGMGIEAERQQDLFLPFVQADGSSTRKFGGSGLGLALCQGIVKAMGGKIGV
                     KSIKDVGSSFYFELPVEIPHGQTSLAEQMRRGFQAYQSGQRQAGAAVLLIGDLPATQM
                     VIQIACQQWGLEFHWEPKEAR"
     gene            2674..3759
                     /gene="mutY"
                     /locus_tag="VFX11_04215"
     CDS             2674..3759
                     /gene="mutY"
                     /locus_tag="VFX11_04215"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX4937855.1"
                     /translation="MIAPADFQLRLFQWFDRHGRKHLPWQQAISPYRVWVSEIMLQQT
                     QVNTVIPYFERFMDRFPTVESLAEAPEDAVLQHWSGLGYYARARNLHKAAQQVVAEHD
                     GQFPDSVDALAALPGIGRSTAGAIASISMGITAPILDGNVKRVLTRLHAVEGWPGLPK
                     VADQLWQIAERFTPAERTGDYTQAMMDLGATLCTRSKPACGICPFMDVCQARIQGRQA
                     EFPQSKAKKALPERATWMLMLQSGDEILLYKRPQKGIWGGLWSFPEFTTAEDLDARLA
                     LDGLRVASRQDWPPFRHTFSHYHLDIHPVRVELERRAELVGEGSHMADRSMEGAGQVW
                     YNRRLIQELGLAAPVRKLIDQLLQEIP"
     gene            3756..>3870
                     /locus_tag="VFX11_04220"
     CDS             3756..>3870
                     /locus_tag="VFX11_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HEX4937856.1"
                     /translation="MSRTVFCRKYQKEMEGLPAPPYPGPRGKDIFEHVSRQA"
BASE COUNT          732 a         1282 c         1157 g          699 t
ORIGIN      
        1 cccgtgcttc cttcggctcc cagtggaatt ccagccccca ctgctggcag gcgatctgga
       61 tcaccatctg ggtggcgggc agatcgccga tcagcagcac cgccgcgcca gcctggcgct
      121 gaccactctg gtaagcctga aagccacgcc gcatctgttc cgccagcgag gtctggccgt
      181 gggggatttc caccggcagt tcgaaataga aggaagaacc cacgtccttg atggacttga
      241 cgccgatctt gccacccatc gctttgacga tgccctgaca cagcgccagc cccagcccgg
      301 agccgccgaa tttgcgggtg gacgaaccgt cggcctgcac gaacggcagg aacaggtcct
      361 gctggcgctc ggcctcgatg cccatgccgg tgtctttcac ttccacatac aaacggtgcg
      421 gcggcaacat ttccgcccac agcaggatcg aaccgtggct ggtgaatttc actgcgttgg
      481 acagcaggct ggtgagcagc tggcgcagct tggtggtgtc ggtgacgatt ttctgcggca
      541 ggttggcttc cagcaccacg ccgatatcca gcccggcgcg ataggcatcc ggcgccacca
      601 gattcacggc ggaatgaaac agttcgtgca cgttgacctg ctcgtggcgg aattccattt
      661 cgccgctttc cagacgggag aaatccatca catcgtccac cagcgccagc aggttgcggc
      721 cggaacgggt ggccagcgcc agcgcctcgc gctgttcggc attcagttcg ctgtcgttga
      781 gcaggtcgag catgcccagc atgccgttca tgggcgtgcg gatctcgtgg ctgacgatcg
      841 ccatgaagcg gctcttgttc tcggcggttt tcacggcgat gtcgcgggca tccgccattt
      901 cctcggatgc tttcacgata tcgttgatat ccaccagcac gccgcgcagg cccaccaccc
      961 gcagaccgtc gatcaccacc gacaccagcg ccttcagcca cacccattgg ccatcgccgt
     1021 ggcgcacctt gaattccaca tagctgtcgc ggccgccgga caccgcctcg gaaaacgccg
     1081 ccatgatgcg ggcgtgatga tccgggtgaa tccagcgctg ccactggccc agcatttcca
     1141 gcacctgatc gggggaatgg cccagcacgt gctccacctg gccggacagc agggtgaccc
     1201 ggggcgggtc gatctgcgct tcccacacca cgcagtcgat ggaactcatg atggtctggt
     1261 agcgttcctt cgattcggtg atttcatcca gataggattt gagatcctgc cgcatgttgt
     1321 cgagcgacac cgccagctgc cccagttcgt cattgctgcg caaagccagc ttgcggtcca
     1381 gattgccgcg ggacagatcc gacgcccagc gcgacagccg cgcaatgggc tcgccgatct
     1441 gccgcgacat gacgtaggcc accacaccga tcagcaggat agccagcagg ccgatcagga
     1501 tggcgcgctc gcggatgaag gcgatgtcgg ccagcgcgtc ggtagcgtcg cgcaccagca
     1561 ccaggctcca gccggtatcc ttgatgttcc tgaactcctc cgccggcatg ttcaccagca
     1621 gcagttccgt gtcgccgcgc cggatatagc gatgaccgga gcggtgcagc agacggtcca
     1681 gcagcagggg attctccgtc acccaccagc gccgaccgta cacccgcagc aggtctgatt
     1741 cgctgatgct gccgtcgtca ctgctgagaa tgaaatccgg cgccgcgatg atgaagccgt
     1801 cgcggtcgat cagcactgcg tatccctgct cgttctggcc gccgggaatc acttcgatgg
     1861 catgaatctg ctgcagcagc tcgcgccagt cgaaactggc cttgagcacg ccgatcactt
     1921 cgtcgcggga aaaactgcgg aagatgggca ccgcgatgct gacgccgtat cctcccagca
     1981 aagggtccaa ccgtagcggt gacatctgcg ccttgccggt cgccttgacc tgctggaacc
     2041 attccgcatc gctcacctgg atgcccagca gatcgcgatc gccagcggct accaccacgc
     2101 cctccagatc caccgctacc agagtgctgt acaccgcata ggcgcgctgg taatcggtga
     2161 gcatgtcgct cactatgccc tggggatcgc cacgctccac ttcctgcatg gccgccagcg
     2221 ccgcccagga gcggatgttt tcctcgctga agccaaaggt gcggtccacc tgggacatgg
     2281 ccagcaacga cgccgcctgc aggcgttcgc cgacctgatt gatgatggtt tcacgggatt
     2341 tgaaataggc gagcgtgcca aagaccacca gcggcgcaag tgacaccacc agcatgaccg
     2401 ccagcagctt gttctggatc gtgggcgcca gccagtggcc gaatctgcgg cgctccatgg
     2461 tcagcccggc accggggggc cgagtttcgg gggttctttc gtcactgagt caaactcgca
     2521 aggaaccaac atactgccgg gatatccgtt aattatagtg ggtttatgcg cctgtctcgg
     2581 gggctaccgg gcagatcttc aaaaagggtg gctcaaaata ttgattttga ttaggctttg
     2641 cgcgttttcc tgaccccgac ggacccgcct gccgtgattg cacccgccga cttccagctt
     2701 cgcctgttcc agtggttcga ccgccatggc cgcaagcact tgccctggca acaggccatt
     2761 tccccctacc gtgtctgggt gtcggaaatc atgctgcagc aaacccaggt gaacaccgtc
     2821 atcccgtatt tcgaacgctt catggaccgc ttccccaccg tggaaagcct ggccgaagct
     2881 cccgaggatg cggtgctgca gcactggtcc ggcctgggct attacgcccg cgcacgcaac
     2941 ctgcacaagg cggcacagca ggtggtggcc gaacacgatg gccagtttcc ggattccgta
     3001 gacgccctgg ccgccctgcc cggcatcggc cgctccaccg ccggcgccat tgccagcatc
     3061 agcatgggca tcaccgcgcc gatcctggat ggcaacgtca aacgggtact gacccgactg
     3121 cacgcggtcg agggctggcc cggcctgccc aaggtggccg accaactgtg gcagatcgcg
     3181 gaacgcttca cacccgccga acgcacgggc gactacaccc aggccatgat ggacctgggc
     3241 gccaccctct gcacccgcag caaacccgcc tgcggcatct gcccgttcat ggatgtgtgc
     3301 caggcgcgaa tccagggccg gcaggcagaa ttcccgcaat ccaaagcgaa aaaagcattg
     3361 ccagagcggg ccacctggat gctgatgctg cagagcggcg atgagatcct gctgtacaag
     3421 cggccgcaaa aggggatctg gggcgggctg tggagctttc cggaattcac cacggctgaa
     3481 gatctggacg cccggctggc actggatgga ctgcgggtcg ccagccgcca ggactggccg
     3541 cccttccgcc acaccttttc ccactaccat ctggatattc atccggtgcg ggtggaactg
     3601 gagcgccggg cggagctggt tggggaaggc agtcacatgg cagaccgttc aatggaaggt
     3661 gccgggcagg tctggtataa caggcgcctg attcaggaac tggggctggc cgccccggtc
     3721 cgcaaactga tcgaccagct tctgcaggaa atcccatgag ccgtaccgtg ttctgccgca
     3781 agtaccagaa agaaatggaa ggcctgcccg ccccacccta ccccggcccc cggggcaagg
     3841 acatcttcga gcatgtgtcg cgccaggcct
//
LOCUS       DASUEG010000223         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1109761, whole genome shotgun sequence.
ACCESSION   DASUEG010000223 DASUEG010000000
VERSION     DASUEG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109761"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1781
                     /locus_tag="VFX11_04225"
     CDS             105..1781
                     /locus_tag="VFX11_04225"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEX4937857.1"
                     /translation="MRTQTQAPVTLPTGYLLQNCRRVLTWVRQLYEDLLTEPERQFLQ
                     DFDTLSTDAQSLYVRLLTRKGPLLAIDSLNYPEVNSTEIALDSLCKAGFATRNPAADA
                     ESLLALLTKPELLTWFADKTAARLPRAELVQYICTQTPLAVIHQTLIQHRPYVRAEHS
                     DIFATCLLLFFGNNRQDLSEFVITDLGHVRYENYALSRETRRFNHRAEIDILRQYFAI
                     DDQLQDEQLLQDAPRLIALQQQLPHHQNLPELQRRHERIALTIARQLERLDQHDAAIA
                     IYRTCQQHPSRERQARILAKQGNPLAALALCHAILDDSHHPEEREFAEKFGATLARKQ
                     QQTFPNIAADADDIDTFHLQLPWQDEPVELLAARALSDTQHSCLYVENSLFCSLFGLY
                     CWDIVYTPLPGAFFHPFQTGPHNLRSEFFYADRREQLDQRFATMDQPGWQERLWHHFL
                     HKQGIANPFVFWDWLTPELIELTLRAIPAPHLQAVFRQLCQHPGFYASGFPDLIRFAF
                     DTGTIPRYELIEVKGPGDRLQANQRRWFRRFRQAGIPARLLTVSWKETGE"
     gene            1778..>2021
                     /locus_tag="VFX11_04230"
     CDS             1778..>2021
                     /locus_tag="VFX11_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937858.1"
                     /translation="MNTNGPRQFRISVTELAEFCCRRGDLNLGLERTPSAQDGQRGQR
                     SVQANRPPAYQREISVNATFGDNVFQCTLAGRIDGLL"
BASE COUNT          434 a          675 c          538 g          374 t
ORIGIN      
        1 aaggaccagc aaggcgtcaa gccccacatg tcgggcacca tcgagtcgct gtaagaacac
       61 acgggccggt aatgccggcc ctttttcgtt gtgccaaacc atttatgcgt acacaaacgc
      121 aggctcccgt cacgcttccc accggttacc tgctgcaaaa ctgccgccgc gtactgacgt
      181 gggtgcggca actgtatgaa gacctgctca ccgaacccga acgccagttc ctgcaggatt
      241 tcgacacgct ttcaaccgac gcgcaaagcc tgtacgtccg cctgctgaca cgcaaggggc
      301 cgctgctggc catcgacagc ctgaattatc cggaagtgaa ctctacagaa atagcgctgg
      361 attccctgtg caaagcagga ttcgccacac gcaatccggc agcggacgcg gaatccctgc
      421 tcgccctgct gaccaaaccg gaactgctga cctggttcgc cgacaaaacc gccgcccgtc
      481 tgccccgcgc tgaactggtc caatacatct gcacgcaaac gccactggcg gtcattcacc
      541 aaacgctgat tcagcatcgc ccctatgtgc gcgccgaaca cagcgacatc tttgccacct
      601 gcctgctgct gtttttcggc aacaaccgcc aggatctgag tgaattcgtc atcaccgatc
      661 tgggccacgt gcgttacgaa aattatgcgc tgtcacggga gacccgccgc ttcaaccacc
      721 gcgccgaaat cgacatcctg cgccagtatt ttgccatcga cgatcaactg caggatgagc
      781 aactgctgca ggacgcgccc cggctgatcg cgctgcagca acaactgccg caccaccaga
      841 acctgcctga attgcagcgt cgccacgaac gcatcgcgct caccattgcc cgtcagctgg
      901 aacggctgga ccagcacgac gcagccatcg ccatctaccg cacctgccag caacatccca
      961 gccgggaacg gcaggcccgc attctggcca aacaaggcaa tccactggct gcgctggcgc
     1021 tatgccacgc gattctggat gacagccatc atccggaaga acgggaattc gccgagaaat
     1081 tcggtgccac cctggcgcgc aaacagcaac agactttccc caacattgct gcggatgccg
     1141 atgacatcga tacttttcac ctgcagctgc cctggcagga cgagccggta gaactgctcg
     1201 ccgcccgcgc cctgagcgac acacaacaca gctgccttta tgtagaaaac agcctgttct
     1261 gcagtctgtt cggcctctac tgctgggata ttgtgtacac gccgctgccc ggcgcttttt
     1321 tccatccgtt ccagaccggc ccccataacc tgcgcagcga atttttctac gccgaccgcc
     1381 gcgaacagct ggaccagcgt ttcgccacca tggaccagcc tggctggcag gaacggctgt
     1441 ggcaccactt cctgcacaaa cagggcatcg ccaatccgtt cgtgttctgg gactggctga
     1501 caccggaact gatcgaactg acactgcgcg cgattcccgc gccacacctg caggccgtgt
     1561 tccgccagct gtgtcagcac cccggattct atgccagcgg tttccccgac ctgatccgct
     1621 ttgcgttcga caccggcaca ataccccgct atgaactgat cgaggtgaaa ggtccgggtg
     1681 atcgtctgca agcgaaccag cgacgctggt ttcgccgttt ccgccaggct ggtattccgg
     1741 caagattgct gactgtcagc tggaaggaaa caggggaatg aacaccaacg gtccgcgtca
     1801 gtttcgcatc agcgtcactg aactggcgga attctgctgc cggcgtgggg acctgaatct
     1861 gggcctggag cggacaccat ccgcgcagga tgggcaacgg ggtcagcgct cggtgcaggc
     1921 taaccggcca cccgcctacc agcgtgaaat cagcgtgaac gcgacttttg gcgataacgt
     1981 tttccagtgc acactggcag ggcggatcga cgggttattg g
//
LOCUS       DASUEG010000224         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_160208, whole genome shotgun sequence.
ACCESSION   DASUEG010000224 DASUEG010000000
VERSION     DASUEG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160208"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..638)
                     /locus_tag="VFX11_04235"
     CDS             complement(168..638)
                     /locus_tag="VFX11_04235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4937859.1"
                     /translation="MATVELDKPVPDFAAQATGMKDIVLSKLKGWKVVLYFYPKDNTP
                     GCTTEGLDFREFYADFLKAKTLVFGVSKDTLKSHEQFKAQHGLPFELISDTDAYLCRM
                     FEVIQLINLQGKQIPGVERSTFLIDSSGVLRREWRKVRVNGHVEEVLEAAEGIS"
     gene            complement(697..1224)
                     /locus_tag="VFX11_04240"
     CDS             complement(697..1224)
                     /locus_tag="VFX11_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4937860.1"
                     /translation="MQEFLVVSAIGEDKPGIINALSQVATESGCNILDTRMTVLGGEF
                     ALLMMLNGTEAAIVKASSQFPAVADRFGLTTILKRTQPRRIDQAVRPYRVTVVALDHP
                     GIVREIAGFFGNRQINIEEMETGTYAAAHTGTPMFSLEMTVNVPATIPIAGLKDAFVT
                     FCDERNLDASIEPRR"
     gene            1513..2388
                     /gene="dapA"
                     /locus_tag="VFX11_04245"
     CDS             1513..2388
                     /gene="dapA"
                     /locus_tag="VFX11_04245"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4937861.1"
                     /translation="MITGSIVALVTPMHADDSIDWDALARLIDWHIEQGTSAIVAVGT
                     TGESATLDVDEHCDVIRFTVERSDRRIPVIAGTGANSTREAIELTQEAKSVGADACLL
                     VTPYYNKPTQEGLYRHHMAIADAVDIPQMLYNVPGRTACDMLPETVARIAVHPNVVAI
                     KEATGDLARGRKILELCGDQIVLYSGDDATAWELILLGAKGNISVTANVAPKAMADLC
                     AAALAGDRARAEAINAPLTDLHKNLFLEANPIPVKWALQRMGLIGPGIRLPLTPLSEK
                     FHQPVTDALKKAGLV"
BASE COUNT          529 a          735 c          765 g          557 t
ORIGIN      
        1 tggccgcggc gggagttgcc acccaattcg acggaatggc tcgatattgc tgacgaatga
       61 aagtggccga gagctggccg aaaaatatgc aacaaaaagg gcggctcgca ggggccgccc
      121 tttttacttt gggctgagtc aacagatgca ggctgtcgaa tgtgcgatca gctgatacct
      181 tccgctgctt ccagcacttc ctccacgtgg ccgttcaccc gcaccttgcg ccactcccgg
      241 cgcagtacgc cactggagtc gatgaggaag gtactgcgtt caacacccgg gatctgcttg
      301 ccctgcaggt tgatcagttg aatgacctca aacatgcggc aaaggtaggc atcggtgtcg
      361 gagatcagtt caaagggcag tccatgctgg gccttgaact gctcatggga cttcagggtg
      421 tctttggaga ccccgaacac cagggttttg gctttcagga aatcagcgta aaactcgcga
      481 aaatccagtc cctcggtggt gcagccgggg gtgttgtcct tgggatagaa atagagcacg
      541 accttccagc ctttgagctt tgaaagcacg atgtctttca tgccggtggc ctgtgccgcg
      601 aaatccggaa ccggtttatc gagttctacg gttgccattg actcctccta agcctgtctt
      661 ggttcttttt gtgagtggaa cacgagctgc accggcttat ctgcgcggct caatcgatgc
      721 atccaggttg cgttcatcgc agaaggtcac gaacgcgtct ttaagtcccg caatcgggat
      781 cgttgccgga acatttacgg tcatttccag agaaaacatg ggtgtacctg tatgggcagc
      841 agcataggtg ccggtttcca tttcttcaat gttgatttgc cgattgccaa aaaaaccggc
      901 aatttcccgt acgatgcctg ggtggtccag tgccaccacg gtgacgcggt agggccggac
      961 agcctgatcg atgcgacgcg gctgggtgcg tttcaggatg gtggtgaggc cgaagcggtc
     1021 ggccacggcc gggaactggc tggacgcctt gacgatggcc gcttcggtgc cattgagcat
     1081 catcagcagg gcgaattcgc cgcccagcac ggtcatgcgc gtgtccagaa tattgcagcc
     1141 cgactccgtc gcgacctgcg acagcgcatt gatgatgccg ggtttgtctt cgccaatggc
     1201 ggatacgacc aggaattcct gcatgtcagc tttccgttct gttcattctg tccgttctgc
     1261 tggtctttcc ccggttggga acgggaattg cggaaccaaa accggtgcgc acatgaccaa
     1321 gtgaatgcct gaaaggagtt tagcacccga tttggggaac aactagctga aaatgctgca
     1381 aatattcaga ttgatcgcgg attttgccgg aaccaggcgc gaattgtggt gccagccgga
     1441 cgggcgcttg ttattgctcc ggtgcatcag taccatagcc gcttgcttga cagggtggag
     1501 aacggaataa acatgattac aggaagcatc gtcgcgttgg tgacgccaat gcacgcggat
     1561 gacagcatcg attgggatgc actggcccgt ctcatcgact ggcacattga gcagggcacc
     1621 agcgccattg tggcggtggg caccaccggt gaatccgcca ccctggacgt cgacgagcat
     1681 tgtgatgtca tccgtttcac tgtcgagcgc agtgaccgcc gcattcctgt cattgccggc
     1741 accggtgcca acagcacccg ggaggccatc gaactgacgc aggaagccaa gtccgttggc
     1801 gccgatgcct gtcttctggt aaccccctat tacaacaagc ccacccagga aggtctgtac
     1861 cgccatcaca tggcgattgc cgacgcggtg gacattcccc agatgctcta taacgtgccg
     1921 ggccgtaccg cctgtgacat gctgcctgaa accgtggccc gtattgccgt tcaccccaac
     1981 gtggtggcga tcaaggaagc cactggcgat ctggcccggg gtcgcaagat cctggaattg
     2041 tgtggcgacc agatcgtgct ctattccggc gatgacgcca ctgcctggga attgatcctg
     2101 ctgggtgcca agggcaatat ttccgtcact gccaacgtgg cgcccaaggc catggccgat
     2161 ctgtgtgccg ccgccctggc cggcgaccgt gcccgcgccg aggccatcaa tgcccccctg
     2221 accgacctgc acaagaacct gttcctggaa gcgaatccga ttccggtgaa atgggccctg
     2281 cagcgcatgg gactgatcgg cccgggcatt cgcctgccgc tgacacccct gtcggaaaaa
     2341 ttccaccagc ccgtgaccga tgcgctgaaa aaagcgggcc ttgtctgatt ccagctcttc
     2401 ggagacgttc atgcgtttga tgcaacacgc gctggtttta cccgtgctgc tggcgatttc
     2461 cggctgtgcc tggctgttcg gcgaggaggg ccagttccgt gagcgctcca tggattacaa
     2521 ggatgcacac agcattgaac cgctgaaagt cccggccggt atggagacga aggagctcaa
     2581 acagcg
//
LOCUS       DASUEG010000225         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_402865, whole genome shotgun sequence.
ACCESSION   DASUEG010000225 DASUEG010000000
VERSION     DASUEG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402865"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..925
                     /locus_tag="VFX11_04250"
     CDS             140..925
                     /locus_tag="VFX11_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX4937862.1"
                     /translation="MTASTFFENYHIGLSLLTWVGIIGMTLLGRKFGPMIPAIGRANA
                     INQEAFNKKMQREFYAANQKLNRKWGLLDQVLIFVLIIPFCVTLDSQPLWTMVLDIFV
                     ILMFYDFFYYLTHRFLFHNGGFLGGPLMWVHAVHHQQNNPCRQDSSYIHPLEVAIGLG
                     LFAGSILVLSLIMGEFHVVTIILSWTLFSEINLHNHDLWEEKRFPWKYLNYMSKMHHY
                     HHAKFTGGNFATISLFYDWLFGTYDRGNGYKKLKQEEGPQKAA"
     gene            1338..1781
                     /locus_tag="VFX11_04255"
     CDS             1338..1781
                     /locus_tag="VFX11_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX4937863.1"
                     /translation="MSDHETKEVQELITILNDSADFYREAHDKVTNAALKETFDEIAG
                     IKEQLVDRLQPLVVLQTGEAEHGHSVSVKVRQIYTNVLASLQKDKAETFIDNLEEVED
                     KTRQQARKAMDAADTTVVAIALRDAFPDIERCHDEMSRLKAARAR"
     gene            1853..2146
                     /locus_tag="VFX11_04260"
     CDS             1853..2146
                     /locus_tag="VFX11_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937864.1"
                     /translation="MTVREEAFARDKAEHQEYTNLKRDVETLRKDLARLSSGLLNDAK
                     TSAEQVLSSVNQKSQDVVRQAEEKIGEKPFLSLLMSFVLGLILAKVLDSRSAR"
     gene            2202..2618
                     /locus_tag="VFX11_04265"
     CDS             2202..2618
                     /locus_tag="VFX11_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937865.1"
                     /translation="MKSMVQWLLICFFLVVGLWLLLFGVLAAGIVLLLKALILALTPV
                     AGLAGAYAASGIVCLVGVALVLLVVWLVMRQATTHQPASLAGPAKVNAYDSATLILKK
                     YPLESVVVAFAAGLSSQDMGELKKMAGMMLRESEGG"
BASE COUNT          633 a          709 c          811 g          653 t
ORIGIN      
        1 agaacattgt tcccaaatga agtgtatctt ttgatgtcag tcaatcattc tcttgcggta
       61 tcgtcgattt ttcgttgact aggactcatt ttgcgattct acttcgtgag aacgctgttc
      121 tttcccgagg ttctatgcga tgactgcatc gacatttttt gagaattacc acattggcct
      181 gtcgctgctg acatgggtcg gcatcattgg catgacgctg ctggggcgaa agttcggccc
      241 gatgattccc gccatcgggc gcgccaatgc gatcaatcag gaggcgttca acaagaagat
      301 gcagcgtgag ttctatgcgg ccaaccagaa gctcaaccgc aaatggggtc tgcttgacca
      361 ggtgttgatt ttcgtgctga tcatcccgtt ttgcgtgacg ctggattcgc aaccgctgtg
      421 gacgatggtg ctggatatct tcgtcatcct gatgttctac gatttctttt attacctgac
      481 ccaccgcttc ctgtttcaca atggcggctt tctggggggg ccgctgatgt gggtgcacgc
      541 ggtgcatcat cagcagaaca atccatgccg gcaggattcc agttacattc acccgctgga
      601 agtggccatc ggcctgggcc tgtttgcggg cagtattctg gtattgtcac tgatcatggg
      661 tgaattccat gtggtgacga tcatcctgtc gtggacactg ttttccgaga tcaatctgca
      721 caaccacgat ttgtgggaag agaagcgttt cccctggaaa tatctgaact atatgtcgaa
      781 gatgcatcac tatcaccacg cgaaattcac gggtggcaac tttgcaacaa tctcgctgtt
      841 ctacgactgg ctgtttggca cctacgatcg tggcaacggc tacaagaaac tcaagcagga
      901 agaggggcca cagaaggcag cctgatgcgg cacggatatc cagtgcggct caggatataa
      961 gtggtcgtga tgaaagtcct gaaggcctgt gcgggatgaa gtcggtcccg cagtctgcca
     1021 tttttttgcg ccagtctttt tttgcatttt atgcaatccc ctccattgac gctgtaagaa
     1081 ttgccgggca agggatgccc ggtacgttcg ccatccagtt cacttcgccg cacaaccctg
     1141 acaatactgg ctgcagcccg cgccagccgt gctctgcgcg cgacatcttc agatacgggt
     1201 aatgccaggc acggaagttg ccttactgtc tgcgtacgat acgaaaagcg ggtttgtagc
     1261 catcgctcca acgggagaca cgctggccac aagcccacca gccatcatca tgttgcagaa
     1321 cggaatggga gaagaccatg tcggaccatg aaacaaagga agtgcaggaa ctcatcacga
     1381 tactgaatga cagcgccgat ttttatcggg aagcccatga caaggtgacc aatgcagccc
     1441 tcaaggaaac gtttgacgag atcgccggta tcaaggagca gctggtagac cggttgcaac
     1501 cgctggtggt gctgcagacc ggggaagcgg aacacggtca cagtgtatcg gtcaaggtgc
     1561 gtcagatata caccaatgtt ctcgcctcgt tgcaaaaaga caaggcggaa acctttatcg
     1621 acaatcttga agaagtggaa gacaaaaccc gccagcaggc gcgcaaggct atggatgcgg
     1681 cagacacaac tgtcgtggcc attgcgctgc gcgatgcttt tccggatatc gagcgttgcc
     1741 atgatgaaat gagccggctg aaggcggccc gcgcccgtta gccacgtccc gtggcatgag
     1801 ggcgggcgcg aaccgtcctg cagataacct ttccaattca aggagaacca ccatgactgt
     1861 cagagaagaa gcgtttgcac gcgacaaggc cgagcatcag gaatacacca atctgaagcg
     1921 ggatgttgaa acactgcgca aggatctggc caggctgtcg agcggacttc tgaacgatgc
     1981 gaaaaccagc gccgagcagg tgctgagcag cgttaaccag aaatcccagg acgttgtgcg
     2041 ccaggccgag gaaaaaatcg gcgagaaacc tttcctcagt ctgctgatga gcttcgtgct
     2101 gggtttgatt ctggccaagg tgctggactc gagaagcgcg cggtagtgtt ggcgaagcac
     2161 aatgccggag ccgcagcagg atcaatcagg agaacgaacg gatgaaaagc atggtgcagt
     2221 ggttgctcat ttgcttcttt ctcgtggttg gtctgtggct tttgctgttc ggcgtattgg
     2281 ctgcgggaat cgtgctgctg ctgaaggcgc ttatactcgc attgacgcca gtggcgggtt
     2341 tggccggggc ctatgcggcc agcggtatcg tatgcctggt gggtgttgcc ctggtcttac
     2401 tggttgtatg gctagtcatg cggcaagcga caacgcatca acctgcatcg cttgccggcc
     2461 cggccaaagt gaacgcgtac gattccgcca cactgattct gaagaaatat ccgctggaat
     2521 cggtcgtggt ggcgtttgcc gccggccttt cgtcccagga tatgggcgaa ctgaaaaaaa
     2581 tggcagggat gatgttgcgg gagagcgaag gcggctagag ccattgcctg caacgctgga
     2641 tttgctgtgc gatcaggcat cgttcaggtt tttcgccggt tcgtccgttg ccgtcccatg
     2701 aaaacagatc gcatgtcgtc cggttacgag gagaaagtca catggacagt cattccgttt
     2761 ttggaattta cgacgaatat cccgacgcca cggcggctgg ttttgg
//
LOCUS       DASUEG010000226         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_203920, whole genome shotgun sequence.
ACCESSION   DASUEG010000226 DASUEG010000000
VERSION     DASUEG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203920"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2527)
                     /locus_tag="VFX11_04270"
     CDS             complement(194..2527)
                     /locus_tag="VFX11_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX4937866.1"
                     /translation="MTATAIQSIFGETKADLLVEPASECRTPKLSLHQFIADFGDDLL
                     QAVERELPPVYQGQRPAVRDLALDSLKRKPFDAQRETVHAATTLLVDRDEQACVLNCD
                     MGTGKTMMAICISVLLHQAGYKRMLVLSPPHLVYKWRREILDTVPGAVVWVLNGPDTL
                     AKLLKLRESLDVTVKGPEFFILGRVRMRMGFHWRVAHGVRHRYSMDAEDGNNANSRKV
                     LSKETYACCPDCGAPIVINESGRYASPEDMTCFDRRIGCPECGARLWTLMRPKAQRSR
                     RDLVLDTVQQLPTIGPKTANRLINLFGEDSLADMLSDNLYSFINLMDDEGNLVFSDRQ
                     AKRMERALANMELSFGDGHYQATEFVKRYLPKSAFDVLVVDEGHEYKSGDSAQGMAMA
                     VLTTQVRKTLLLTGTLMAGYADDLFFLLWRILTRRMLEDGYRYNSRGTLGSASMAWMR
                     EHGVLKDIYKETEGGDHRTAKGKRISVRTSKAPGFGPKGIARYILPFTVFLKLKDIGQ
                     NVLPSYDEIFHEVAMTSEMDGIYRRMKEDLSDMLKKALRVGDHTLMGVVLNALLAWPE
                     CCFRPEQITHPRDRRNVLHFNAALFGEGEPTPKEQALVDICRQERSQGRRVLIYTVYS
                     GTRDTTTRLKMLLAREGFQASVLKATVPTDKREDWIIDQVERGCDVLITNPELVKTGL
                     DLLDFPTIVFMQTGYNVYTLQQAARRSWRIGQKWAVRVIFLGYQGTAQIQCLQLMAKK
                     IAVSQSTSGEMPESGLDILNQEDDSIEVALAKQLIAA"
     gene            complement(2629..3210)
                     /locus_tag="VFX11_04275"
     CDS             complement(2629..3210)
                     /locus_tag="VFX11_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937867.1"
                     /translation="MLNLDFSSSVFCDGLLTNQDGELLFMSVWGRDTDITELLARLTL
                     PDHPDGIRSFNAKGDGHAMKVVVPNPKLLDKQQGRAAGSVFGELIQLFVFARSLQRPD
                     RVSREAWALFRVRDGQPDIPDVWPLVMETCHVPLLPQWREPVLAAFRYRGWISQVAGY
                     RMGALGVHLGNDNVELCIEDLVRTRQIGLSNAF"
     gene            complement(3220..4371)
                     /locus_tag="VFX11_04280"
     CDS             complement(3220..4371)
                     /locus_tag="VFX11_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HEX4937868.1"
                     /translation="MALMFSRIARNFIKNGYFPTDEDTLQRVLSALVPQSDQPIRICD
                     PCCGEGVALAEAQHYLTSLGATVTSYGIEYDEERAWHAKQLLSRCIHADLNDCKVSLR
                     TFSLLWLNPPYGDLVSDSERVMTNRSGGRKRLEKEFFQRCIHMLQPGGVLVLIVPYTV
                     LDQEFSGWISKYCRDVSVFAAPEQQFRQAVILGVKRSRAVEPDKRLVLQMTQAQKNLY
                     CDTEGHWVDGFSVHCQLPEVWNKAPYEVPASAGSPEIDFSTIKLDVKQLEAEIAAAPV
                     LWGRFDTLFRTRTDTARQPLRKLSDWHLALMLAAGQVSGVVTGKSGRVLLVKGDTLKT
                     KNIRQETELNEETGSVTLTRIATDRFVPLIRGIDFTPGGAFFGRIIHIE"
     gene            complement(4466..4771)
                     /locus_tag="VFX11_04285"
     CDS             complement(4466..4771)
                     /locus_tag="VFX11_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937869.1"
                     /translation="MNYTLLDGYRCDIASLETGKMYLRLTGGREDLSVRPDLPQDDDP
                     VFGPLTWFKLFDTETCGIEFQDGGCYLIIETGPVLHFRSGMIWYQKFYYSPVLFVAG"
     gene            complement(4768..>5598)
                     /locus_tag="VFX11_04290"
     CDS             complement(4768..>5598)
                     /locus_tag="VFX11_04290"
                     /inference="COORDINATES: protein motif:HMM:NF023108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HEX4937870.1"
                     /translation="VRFHSIPSFFFKPNFPTNPSGDANSPWGHCVSGFQLRSQEHTMS
                     IMLEGTLAVQRISGVNGEFSVGKLETEVGVLNLKDPHLDQYETGKYQGRFLIDRIFPH
                     GYMSRTNCFIVEIRAIVKEYIIHADEPEELDTDVGLTEMDPLEAVPSAIPSPAVSHSA
                     SPAEASRDAVATKGDQSKPAKSVTPPSVKPAPAQTSSGKPTQAPQGTPEEALAELFGE
                     LWPLAETVTLDPTTIRSDAENHRKRTQYLKSQGYIFKATSQTWFKQRSTANIGEAMVL
                     "
BASE COUNT         1359 a         1600 c         1392 g         1247 t
ORIGIN      
        1 aagcggcctt tttagcggat gcaaaacgca tgagccaggt gaaactggcc aagcactacg
       61 gaatcagcat tacaacagcg cgcaagtatt tagcggaatg gcaagagtaa ctcgccgacg
      121 caaaggggct gaatattgga tgcgtgctcg cgcaagcccc accacctgac aggatgtcgg
      181 gtggtgggat ttttcaagct gcaatcagtt gcttcgccaa cgctacttcg atggaatcgt
      241 cttcctgatt gagaatatcc aacccggatt ccggcatttc acccgatgtg ctttgactca
      301 ctgcaatctt ctttgccatt agttgaaggc actgaatctg tgccgtgccc tgatagccca
      361 gaaagatcac tctgaccgcc cacttctgcc caatccgcca ggagcggcgg gctgcttgtt
      421 gcaatgtata aacgttgtaa cccgtctgca taaacacgat ggtcggaaaa tcaagcagat
      481 ccaagccggt cttgaccagt tccggattcg tgatgagcac atcacaaccc cgctccactt
      541 ggtcaatgat ccagtcttcc cgcttgtccg tgggtaccgt cgccttcagc accgatgctt
      601 gaaagccctc acgggccagc agcatcttga gcctagtggt ggtatccctc gtgccgctat
      661 agacggtata gatcagcacc ctcctccctt gcgaacgttc ctgccgacaa atgtcgacca
      721 atgcctgttc cttcggagtt ggttcgcctt cgccaaacag tgccgcattg aaatgcaaca
      781 cgttgcgacg atcgcgcggg tgtgtaatct gctccggccg gaaacaacac tccggccagg
      841 ccagcagcgc attgagcacc acacccatta aggtatgatc ccctacgcgc agggccttct
      901 tgagcatgtc tgacagatcc tccttcattc tccgatagat tccatccatt tcgctcgtca
      961 tcgccacctc atgaaaaatt tcatcatagg atggcagaac gttttgaccg atgtccttca
     1021 acttcaggaa caccgtaaat ggcaggatgt agcgggcaat acccttggga ccaaaccctg
     1081 gggctttgga tgtacgcacc gaaatgcgct tccccttcgc agtgcgatga tcgccgcctt
     1141 cagtttcttt gtagatgtcc ttgagtacgc cgtgttcgcg catccaggcc atcgacgctg
     1201 accccaaagt gccacgactg ttatagcgat aaccatcctc cagcatgcgg cgcgtcaaga
     1261 tacgccacag aagaaaaaac aggtcatcgg catagcccgc catcagcgtg cccgtaagca
     1321 gcagggtctt gcgaacctgt gtcgtcagca ccgccatggc cataccctgt gcgctatccc
     1381 cggacttgta ctcgtgccct tcgtccacca ccagaacatc gaatgcgctc ttcggtaaat
     1441 accgcttcac aaattcggtt gcctgatagt ggccgtcgcc aaagctcaac tccatgttgg
     1501 caagcgccct ttccatccgt ttggcctggc gatcggaaaa caccagattg ccttcatcgt
     1561 ccataaggtt gatgaaggag tacaggttgt ccgacaacat atcggccagc gagtcctcgc
     1621 caaacagatt gatcagtcga ttagccgttt tggggccgat ggtgggtaac tgctgcaccg
     1681 tgtccagcac cagatcccgc cgactccgtt gtgccttggg gcgcatgaga gtccacagtc
     1741 ttgcaccaca ttccggacag ccaatgcgcc gatcgaagca agtcatgtct tccggcgagg
     1801 cataccgacc gctttcattg atgacaatag gcgcaccaca atccggacag caggcatagg
     1861 tctctttcga gagcactttc cggctgttcg cgttgttgcc gtcttccgca tccattgaat
     1921 agcggtgacg tacaccatgc gcaacgcgcc aatggaaacc cattcgcata cgcacacgtc
     1981 ccagaataaa aaactccggg ccttttacag tgacatcgag ggactcccgg agcttcagca
     2041 gctttgccag ggtatccggg ccattgagca cccaaaccac tgcacccggc acggtatcca
     2101 ggatctctcg acgccacttg tagaccaggt gcggtggcga cagcaccaac atacgcttgt
     2161 aacccgcctg atgaagcaag accgaaatgc aaatggccat catggtcttg cccgtgccca
     2221 tgtcgcagtt caacacgcag gcctgttcat cgcgatcgac caacaatgtg gtcgccgcat
     2281 gaacagtctc gcgttgtgca tcgaagggtt tgcgcttaag gctgtccagg gcaaggtcac
     2341 gcaccgctgg ccgctgcccc tgatatacag gtggcagttc ccgctccacc gcctgtaaca
     2401 gatcatcacc gaaatcggca ataaactgat gcagggacag cttgggtgtc cgacactctg
     2461 atgcaggctc taccaacaga tcagctttgg tttcgccaaa aatcgattga atcgctgttg
     2521 cagtcatggt tttctccgtg tgaaaaaaga aaccccgaca gcaacaacac accccaacgg
     2581 gatgcactga tcccgccagg gtggttgatt aaaaaaatga acgttggatt aaaaggcgtt
     2641 actgagacca atttgacgag tacgcaccag atcctcaatg cacagctcga cgttgtcgtt
     2701 gccaagatga acacctagag cgcccatccg gtaacccgcg acctgtgaaa tccagccccg
     2761 gtatcgaaac gcagccagca ccggttcccg ccattgaggc agcaacggga catggcaggt
     2821 ttccattacc aaaggccaga catcaggaat atccggttga ccatcccgaa ccctgaacag
     2881 tgcccaggct tcacggctga cacgatccgg acgttgcagt gatcgcgcga acacgaacaa
     2941 ttgaatcaac tcaccgaaaa cactgcccgc tgccctgccc tgttgcttgt ctagcagttt
     3001 gggatttggg actaccactt tcatggcatg tccgtcccct ttggcattga aactgcgaat
     3061 gccatcggga tgatcaggca gggttagccg cgccaatagt tcagtaatgt ccgtatcacg
     3121 tccccagact gacataaaca gcagttcgcc atcctggttg gtgagcagac cgtcgcagaa
     3181 cacagatgaa ctgaaatcca gattcaacat agggaaacct cactcgatat gaatgatgcg
     3241 gccaaagaag gcaccgccgg gtgtgaaatc gattccgcga atcaacggta caaatctatc
     3301 cgttgcaata cgggtcaacg tcacactgcc ggtttcttca ttcaactcgg tttcctgccg
     3361 aatgtttttg gttttgagag tatccccttt taccaacaac acccgtccgg attttcccgt
     3421 taccacaccc gacacttgcc ctgcggccaa catcaacgcc aggtgccaat ctgacaactt
     3481 gcgtaaaggc tggcgtgcag tatcggtacg ggtgcgaaag agcgtatcga agcgccccca
     3541 cagtaccggg gcggccgcga tttccgcttc cagttgcttg acatcgagct tgatggtact
     3601 gaaatcaatt tctggtgatc ctgcgctcgc gggtacctca taaggcgcct tgttccagac
     3661 ctccggcaat tggcaatgca cgctgaaacc atcgacccag tgaccctctg tgtcgcagta
     3721 gaggttcttc tgggcctgcg tcatctgcag taccaatcgt ttatctggct caacagcacg
     3781 agacctttta acacccaaga ttaccgcttg acgaaactgc tgttcaggag cggcaaatac
     3841 agaaacatcc cggcagtact tcgagatcca gccggaaaat tcctgatcca ggacggtata
     3901 gggaacgatg agcaccaata caccaccggg ttgcagcatg tggatacagc gctggaaaaa
     3961 ctctttctcc agacgcttgc gcccaccgct gcgattcgtc ataacacgtt ctgaatcgct
     4021 gaccagatcc ccatagggtg gattcagcca gagcaacgaa aatgtacgca ggctgacttt
     4081 gcaatcattg aggtcggcat gaatgcaccg actcaacaac tgcttggcat gccaggctcg
     4141 ctcttcgtca tactcgattc cgtaggatgt gacggttgcc cccagacttg tcagatagtg
     4201 ctgtgcttct gcaagggcaa caccctcacc gcagcatgga tcacagatgc ggatcggctg
     4261 atccgactgc ggtaccagtg ccgacagcac ccgctgcaga gtatcttcgt ccgtcggaaa
     4321 ataaccgttc ttgataaagt tgcgtgcgat acgtgaaaac attaatgcca tgcgagttgc
     4381 tccgtgtgaa tgaaagaaag ccaacgacgg agcaccacct gacggtaatg aaccgccgat
     4441 ggcgtaaggg gtgagaaacg cagtattaac cggccacaaa cagaaccggg ctatagtaga
     4501 acttctgata ccagatcatg ccgcttcgaa agtgcaacac tggaccagtc tcgataatca
     4561 ggtaacagcc accatcctga aattcaattc cacaagtttc agtatcgaac agcttgaacc
     4621 acgtcagtgg cccaaaaaca ggatcatcat cttgtggcaa atccggccgc actgaaaggt
     4681 cttccctgcc accggtcagg cgtaagtaca tctttccggt ttcaagagat gcaatatcac
     4741 aacggtaacc atcgagaagc gtatagttca tagaaccatt gcctcaccaa tgttggccgt
     4801 actacgttgt ttgaaccagg tctgacttgt cgccttgaag atatatccct gcgacttcag
     4861 atactgcgtc cgcttgcggt ggttttccgc atcggaacgt atcgtggtcg gatccagcgt
     4921 gacggtttca gccaaaggcc acaactctcc aaacagttca gccagtgcct cctctggggt
     4981 accttgcggt gcctgagttg gtttgccgct gctcgtttgt gccggtgctg gtttgacact
     5041 gggtggcgta accgatttgg cgggcttgga ttgatcaccc ttggtcgcca cagcatcccg
     5101 cgaggcttca gccggtgatg ctgagtgcga aactgccggt gagggaatgg cagaaggcac
     5161 cgcttccagc ggatccattt ccgtcagacc aacatccgta tcgagctctt caggttcgtc
     5221 cgcatgaata atgtactctt tgacgatggc cctgatttcc acgataaagc aattggttct
     5281 ggacatgtat ccatgcggaa aaatgcgatc gatgagaaat cgcccttgat acttaccggt
     5341 ttcatattga tccaggtggg gatctttgag attgagaacc cccacttccg tttccagctt
     5401 gccaacggag aattcaccgt tgacccctga aatacgctga acagcgaggg taccttcaag
     5461 catgattgac attgtgtgct cctgtgaacg caattgaaat ccggagacgc aatgacccca
     5521 cggggaattg gcatcccctg atgggttggt aggaaagttg ggtttgaaga aaaaagatgg
     5581 aattgagtga aagcgaac
//
LOCUS       DASUEG010000227         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1352647, whole genome shotgun sequence.
ACCESSION   DASUEG010000227 DASUEG010000000
VERSION     DASUEG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352647"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX11_04295"
     CDS             <1..211
                     /locus_tag="VFX11_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937871.1"
                     /translation="DPLIVAVRLVPDLDGDGDVDNNDMLIVTAARNTAALNNDPRDIN
                     RDGIINVTDARALSLQCTRARCAIN"
     gene            228..905
                     /locus_tag="VFX11_04300"
     CDS             228..905
                     /locus_tag="VFX11_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX4937872.1"
                     /translation="MNSLFMKNRFVKNRIVKRTATLKNNESRKKSALKQAIVCALLTG
                     SLLGAGSASALTVEMVPATGSIALGGSLQIDVRVSDLTDLAAPSLGAYDLNVLFDAAA
                     LQYSHVSWGNQLDLEQLGSLTLEDSGNAGNGLLNLAEISYDTIGTLNDLQAGNITLFS
                     LFFTAVALGNTQVGLDVLALSDAGGSALTADAVSGAQVAVVPVPAALPLLASGLLLLL
                     GRMRRRG"
     gene            1216..>2356
                     /locus_tag="VFX11_04305"
     CDS             1216..>2356
                     /locus_tag="VFX11_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937873.1"
                     /translation="MDLTIKPEYRQFREEVRAFLEANLTPDLREAGRLATSVFHDVER
                     SMAWQKILHKKGWAAPHWPQAYGGTGWDVVQRAIFAEECLMANAPAGIPMGLHMCGPC
                     IIGCGTQEQKDYYLPRILSGEDFWCQGYSEPGAGSDLASLQCSAESDGDDYIINGTKI
                     WTTYAHYANKIFCLVRTNKNCKPQEGITFLLIDMNTPGITVEPIIGLDMIREQNTVFF
                     DNVRVPKANRIGAENAGWTVAKYLLMFERGGQEYAPALHYALSRVKEIAEQEAGSFGG
                     KLSDDAAFMRRVHEVEIEITALEFTENRIKAALGSGQSPGALASMTKILGTEISQKVT
                     ELAIEAVGVYGLPLQVEAIQPGARLPFVGPEYALTAMPRYLNTRAT"
BASE COUNT          465 a          728 c          726 g          437 t
ORIGIN      
        1 tgatccgctg atcgtggccg tgcgcctggt gcccgatctg gatggcgacg gcgatgtcga
       61 caacaacgac atgctgatcg tgacggcagc acgcaacact gctgcgctca acaacgatcc
      121 ccgcgacatc aaccgcgacg gcatcatcaa cgtgacggac gcccgcgccc tgtcactgca
      181 atgcacccgc gcccgctgcg ccatcaactg attgggggag tctctttgtg aatagtctct
      241 ttatgaaaaa ccgctttgtg aaaaaccgta ttgtgaaaag aacggcaacg ctgaagaaca
      301 acgaaagccg caaaaaatcc gctctgaagc aggcgatcgt gtgcgcactg ctgactggct
      361 cgctgctggg tgcaggttcg gccagcgcgc tgaccgtcga gatggtgccg gcgacgggca
      421 gtattgcgct gggtggcagc ctgcagatcg acgtgcgtgt cagcgacctg acggatctgg
      481 cagcgccgtc cctgggagcc tacgacctca atgtgctgtt cgacgctgcc gcgttgcaat
      541 acagccatgt cagctggggc aaccagcttg acctggaaca actgggcagc ctgacgctgg
      601 aagacagcgg caatgccggc aacgggctgc tcaatctggc ggaaatttcc tacgacacca
      661 tcggcacgct gaacgatctg caggccggca acatcaccct gttcagcctg ttttttaccg
      721 ccgttgcatt gggcaatacg caggtggggc tggatgtgct ggcgctgtcg gatgccgggg
      781 gctccgcgct gaccgcagac gcagtgtcgg gcgctcaggt tgcggtggtg ccggtgccgg
      841 cagcactgcc gttgctggcc agcggactgc tgctgttgct gggccggatg cgccgtcgcg
      901 gctgagggct cgcacgatcc ggacagcggg cgctgtactc tggtgcccgc tgtcagccgg
      961 cggtttccag ccagcgccgt cctgctggcc gctttcccgc tcaactcccc ctgtccactg
     1021 acccggttga tgccgccccc cgatcttttt tgccatcgac tgaccagtcg cacccatgca
     1081 caatgacatg cgggcttggg tccggcgttg atctgtctgc cgtttcgccc cccattattc
     1141 gaacaagcaa ccagttggac attgggtttg gacgctgcct ccgtgccgtc cggattcccc
     1201 tgaggaaacc gatttatgga tctgaccatc aagccggaat accggcagtt ccgcgaggaa
     1261 gtgcgcgctt ttctggaagc caacctgacg cccgacctgc gtgaagcagg ccggctggcc
     1321 accagcgtgt tccatgatgt ggagcgcagc atggcgtggc agaaaatact gcacaagaaa
     1381 ggctgggctg cgccgcactg gccgcaagcg tatggcggta ccggctggga cgtggtgcag
     1441 cgcgccattt tcgccgagga atgcctgatg gcgaacgcgc ctgccggcat ccctatgggc
     1501 ctgcacatgt gcggcccctg catcatcggc tgcggcaccc aggagcagaa ggattactac
     1561 ctgccgcgca tcctgtccgg cgaggatttc tggtgtcagg gttattccga accaggtgcc
     1621 ggttccgacc tggcgtcgct gcagtgttcg gcagaatccg acggcgacga ctacatcatc
     1681 aacggcacca agatctggac cacctacgcg cattacgcca acaagatttt ctgtctggtg
     1741 cgcaccaaca agaactgcaa gccccaggaa ggcatcacct ttctgctgat cgacatgaac
     1801 acgcccggca ttaccgtgga gcccatcatc ggcctcgata tgatccgtga gcagaacacc
     1861 gtgtttttcg acaacgtgcg ggtgcccaag gccaaccgca tcggcgcgga aaatgccggc
     1921 tggaccgtgg cgaaatacct gctgatgttc gaacgcggtg gccaggaata cgcgcccgca
     1981 ctgcattacg cactgagccg ggtgaaggaa atcgcagagc aggaagccgg ttcgttcggc
     2041 ggcaagctga gcgacgatgc cgcgttcatg cgccgcgtgc acgaagtgga aatcgaaatc
     2101 accgcgctgg aattcaccga gaaccgcatc aaggcggcgc tgggcagtgg ccagagcccg
     2161 ggcgcgctgg catcgatgac aaaaatcctc ggcaccgaaa tcagccagaa agttaccgaa
     2221 ctggcgattg aagccgtggg tgtttatggc ctgccgctgc aggtggaggc gatccagcct
     2281 ggcgccaggc tgccgttcgt ggggccagaa tatgccctca ccgcgatgcc gcgttatctg
     2341 aacacacgcg ccacca
//
LOCUS       DASUEG010000228         5732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1280469, whole genome shotgun sequence.
ACCESSION   DASUEG010000228 DASUEG010000000
VERSION     DASUEG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280469"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFX11_04310"
     CDS             <1..804
                     /locus_tag="VFX11_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937874.1"
                     /translation="GLERKQVQLDDGTDYVYLEGGTGEPLLLLHGFGADKDNFTRVAR
                     HLTPHYRVIIPDHVGFGESSHPPQADYSPQAQASRLQALMRKLGVTQLHIGGNSMGGH
                     IAITYAAQFPAEVKSLWLLDSGGVWSAPGNDFPKMVRETGVNPLMVRSEEDFARMFDY
                     VMADPPFVPRPILNVKARERIRNFDLEQIIFTQVSADAVEERAATVAVPTLIVWGRED
                     RALNYAAGELLDRVMVNSQLIIMDGVGHAPMLERPAQSAADYLQFRGSF"
     gene            complement(896..2809)
                     /gene="dxs"
                     /locus_tag="VFX11_04315"
     CDS             complement(896..2809)
                     /gene="dxs"
                     /locus_tag="VFX11_04315"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX4937875.1"
                     /translation="MFNEIPLTRPVTPLLDQIERPEQLRALDESLLPQLAQEVRAFMM
                     YAVGKTGGHFGAGLGVVELTIALHFVFNTPADKLVWDVGHQTYPHKILTGRRDRMLSM
                     RQKGGIAGFPKRCESEYDTFGVGHSSTSISAALGMALAARMQGRPDKTVAIIGDGALT
                     AGMAFEAINHAAHTKADMLIILNDNEMSISENVGGLSNYLSRVWASRTYNSLREGGKK
                     VLEKMPAAMEFVRRTEEHMKGMVSPGTLFEELGLNYIGPIDGHNLDKLVTYLGKLRDI
                     PGPHLLHVITQKGRGYQPAEKDPIGYHALSKIEPKPPLAAAKSKPAQPKYCDVFGQWL
                     CDMAEADPRLVAITPAMREGSGMVEFSRRFPEQYQDVAIAEQHAVTLAAGLACEGQKP
                     VVAIYSTFLQRAYDQLIHDVALQNLDVTFGIDRAGLVGEDGPTHAGAYDLSYLRCIPN
                     MVVMAPSDENECRQMLYTAYQHRGPAAVRYPRGTGPGVPVQQALTALPMGKGRIVQPG
                     KGVAILAFGTVLANCKDAGKAFGATVVDMRFVKPIDEALILELAASHHLLVTVEENAV
                     MGGAGSAVNELLAQRNLVLPVLNLGIPDIYVDHAKPAEMIEQCGLHAGGIETAIRVRL
                     ADLEADTLNQTHI"
     gene            complement(2943..3845)
                     /locus_tag="VFX11_04320"
     CDS             complement(2943..3845)
                     /locus_tag="VFX11_04320"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX4937876.1"
                     /translation="MSNTEFDLKSYQAHCQQRVNTRLELWLARQNGLAPRLVDAMRYA
                     TFNGGKRVRPVLAYAAAEALGGNPDHVDDIAAALEMVHSYSLVHDDLPAMDDDDLRRG
                     KPTCHIAFDEATAILAGDALQCLAFEVLTRSDAPLDALIRLQLIQALATASGTRGMAG
                     GQALDLMGEGKTLSLEQLETIHAHKTGSLIRASVRMGALTVPGVTTAQLDALDRYAGA
                     IGLAFQVHDDILDVVGDTAVLGKQQGADAALAKATYPAILGLQQAQQLARDLCDQALA
                     ALQSLDGRAEPLRALARYIVSRQH"
     gene            complement(3842..4087)
                     /locus_tag="VFX11_04325"
     CDS             complement(3842..4087)
                     /locus_tag="VFX11_04325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX4937877.1"
                     /translation="MAKKKSAVDFEKSLQTLEQLVEKMESGELTLEKSLEAFEQGIRL
                     TKECQQALQEAEQKVQILMSRNGSDELQPFSESDDDQ"
     gene            4204..4887
                     /locus_tag="VFX11_04330"
     CDS             4204..4887
                     /locus_tag="VFX11_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4937878.1"
                     /translation="MAFSRRMLCWLLAGGCVLVLSLPVRATEPAPAATGSPPASGRAA
                     AVMKRLTGWTRADAAWLAAQLQADIGRGSLEVEAQKRRILVRVLDRDAFGAGTATLRP
                     SFQPVMDRVRVAIRQLQGDISVEGHTDDIPVNNARFRSNWELSASRAAAVAQDLLQQG
                     EFEPTRFRVVGYGNTRPLVANDTEEHRSLNRRVEIVIDQGRDDAAYLSGAKPLPAAQR
                     PASAADELF"
     gene            4981..>5732
                     /locus_tag="VFX11_04335"
     CDS             4981..>5732
                     /locus_tag="VFX11_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4937879.1"
                     /translation="MVESTLAALDRSGAHVIYAALLADLLAERGIGVDALLHNTGIAP
                     ADLKSIDGRLSTRQYIALIRNARRLANDPALAFAFGLHLKLSTHGFLGFAAMSASTLG
                     EALELAVKYCRTRFEFMRLLYYRQQGFGVLEIDEAVPMGEAYLFMLEAFMTSFAVMSM
                     NLLGEIPRDIQVRRTGDKPLHFDDAMRYFQQGHAVRFNDTVNQILIPEVHLQRKLVLS
                     DPVARQLAEAQCEKELQTIQVQDDLVYRVTRLL"
BASE COUNT          987 a         1725 c         1861 g         1159 t
ORIGIN      
        1 ggcctggagc gcaagcaggt gcagctggat gacggtaccg actatgttta tctggaaggt
       61 ggcacgggcg agcccttgct gctgctgcac ggcttcggcg ccgacaagga caacttcacc
      121 cgtgtggccc gtcacctgac accgcattat cgtgtgatca ttccggatca cgtgggattc
      181 ggcgaatcgt cccatccgcc gcaggcggat tattcgcccc aggcccaggc cagccggttg
      241 caggcgctga tgcgcaagct gggcgtgacc cagctgcata tcggtggcaa ttccatgggc
      301 ggacacattg cgattaccta tgccgcacaa ttcccggcgg aagtgaaaag tctgtggctg
      361 ctggattcgg gcggcgtgtg gagcgcgccc ggcaatgatt ttccgaaaat ggtacgcgag
      421 accggtgtca atccgctgat ggtccgcagc gaagaggatt ttgcgcgcat gttcgattac
      481 gtgatggcgg acccgccgtt tgtgccgcgc ccgatactga acgtgaaggc tcgcgagcgc
      541 atccgcaatt tcgatctgga gcaaatcatt tttacccagg tcagcgcgga tgcggtggag
      601 gagcgggcag ccacggttgc tgtgccgacg ctgattgtgt gggggcgtga ggaccgggcg
      661 ctgaattatg ccgctggcga gttgctggat cgggtgatgg tgaattcgca actgatcatt
      721 atggatggcg tggggcatgc gccgatgctg gagcgaccgg cgcagtcggc agcggattat
      781 ctgcaatttc gcggcagttt ctgatagcgg gcattttttc agcgtgcaaa ataaaaaggg
      841 cgcggtcatc aactgcgccc ttttttgttg aggcttttgg cctgttgtca gccgttcaga
      901 tgtgcgtctg atttaatgtg tccgcttcca gatccgccag acggacacgg atggcggttt
      961 caataccgcc agcatgcagt ccgcactgtt cgatcatttc tgccggcttg gcgtgatcca
     1021 cgtaaatatc cggaatgccc agattcagca ccggcagcac cagattgcgt tgcgccagca
     1081 gttcgttcac cgccgaaccg gcgccaccca tcacagcatt ttcctccacc gtcaccagca
     1141 ggtgatggct cgccgccagt tccaggatca gtgcttcgtc gatcggcttc acaaaacgca
     1201 tgtccaccac agtggcgccg aaggccttgc cggcgtcctt gcagttggcc agcacggtgc
     1261 cgaacgccag gatcgccacg cccttgccgg gttgcacgat gcggcccttt cccatgggca
     1321 gggcggtcag cgcctgttga accggcactc cggggccggt accgcgcgga taacgcactg
     1381 cggcgggccc gcgatgctga tacgccgtgt acagcatctg ccggcattcg ttttcatccg
     1441 acggggccat caccaccatg ttgggaatgc agcgcagata actcagatcg taggcaccgg
     1501 catgcgtagg tccatcttca ccaacgagcc cggcgcggtc gatgccgaaa gtcacgtcca
     1561 gattctgcag cgccacgtcg tggatcagtt gatcgtacgc ccgctgcaag aacgtggaat
     1621 aaatggccac caccggcttt tggccttcgc aggccaggcc tgccgccagt gtcaccgcat
     1681 gctgctcggc aatcgccaca tcctggtatt gctctggaaa ccggcgcgag aattccacca
     1741 tgccggagcc ttcgcgcatc gccggcgtga tcgccaccag acgtggatcg gcttccgcca
     1801 tatcgcacag ccactggccg aacacatcgc agtatttggg ctgtgccggt ttgctttttg
     1861 cggcggccag cggcggtttg ggctcgattt tggatagcgc gtgataacca atgggatcct
     1921 tttccgccgg ctggtagcca cggcctttct gcgtgatcac gtgcagcaga tgcgggccgg
     1981 gaatatcgcg cagcttgccc agatacgtca ccagcttgtc caggttgtgg ccgtcgatgg
     2041 ggccgatgta gttcaacccc agttcctcaa acagtgtgcc cggcgacacc atgcctttca
     2101 tgtgctcttc ggtgcgacgc acgaattcca tcgccgccgg cattttttcc aatactttct
     2161 tgccgccttc gcgcaaggaa ttgtaggtgc ggctggccca gacccgggac aggtaattgg
     2221 acagaccgcc gacgttttcc gaaatcgaca tctcgttgtc gttgaggatg atcagcatgt
     2281 cggccttggt gtgggcggcg tggttgatgg cctcgaacgc catgccggcg gtgagggcgc
     2341 catcgccgat gatggccacg gttttgtcgg ggcggccctg catgcgggcg gccagggcca
     2401 tgcccagggc cgcgctgatg gaggtgctgg aatgaccgac gccaaaggtg tcgtactcgc
     2461 tctcgcagcg cttgggaaag ccggcgatgc cgcccttctg gcgcatgctc agcatgcggt
     2521 cgcggcggcc ggtgaggatt ttgtgagggt aagtctgatg gcccacgtcc cataccagtt
     2581 tgtcggcggg agtgttgaag acgaagtgca gggcgatggt gagttccacc acacccaggc
     2641 cggcgccgaa atgcccgccg gtcttgccca cggcatacat catgaaggcg cgcacttcct
     2701 gcgccagctg gggcagcagg gattcatcca gggcccgcag ctgctccggc cgctcgatct
     2761 ggtccagcag cggcgtgacg gggcgggtca gggggatctc attgaacatg ctgcagcctt
     2821 gcggaggtgt ccgggcgggt gggcgaaaaa gtaacgctta ttataggggg tgtccggttc
     2881 gcacacaccc cttgcttgaa atgaccggtg aaaaaagtgc ccggttcggc aagcccgccg
     2941 gctcagtgct ggcgggatac gatgtagcgg gcgagggctc gcaggggctc cgcccgacca
     3001 tcgagggact gcagggcggc aagagcctga tcgcacaggt cccgggccag ttgttgcgcc
     3061 tgctgcagcc ccagaatggc agggtaggtg gccttggcca gggccgcatc ggcaccctgc
     3121 tgtttgccca ggacggcggt gtcacccacc acatcgagga tatcgtcatg cacctggaag
     3181 gccaggccaa tggcaccggc atagcggtcc agcgcatcca gttgcgcagt ggtgacgcca
     3241 ggcacggtca gcgctcccat gcgcacgctg gcacggatca ggctgccggt cttgtgggcg
     3301 tggattgttt ccagctgttc cagtgacagg gtcttgcctt cacccatcag gtccagtgcc
     3361 tggccgccgg ccatgccacg ggtgccggaa gcggtggcca gcgcctggat cagttgcagc
     3421 cggatcagcg catccagcgg cgcgtcggag cgggtgagca cctcgaaggc caggcattgc
     3481 agcgcgtccc ccgccaggat ggcggtggct tcgtcgaagg cgatatggca ggtgggcttg
     3541 ccgcggcgca gatcgtcgtc atccatggcg ggcaggtcgt cgtgcaccag ggaataggag
     3601 tgcaccattt ccagggcggc ggcgatgtcg tccacgtggt cagggttgcc acccagcgct
     3661 tccgcagcgg cgtaggccag taccggtcgc acccgcttgc cgccgttgaa agtggcgtag
     3721 cgcatggcgt ccaccagccg gggcgccagg ccgttctgcc gggccagcca gagttccagc
     3781 cgggtgttga cccgttgctg gcagtgggcc tggtaggact tcagatcgaa ttcggtattg
     3841 ctcattgatc gtcgtcgctt tcgctgaagg gttgcaattc gtcgctgccg ttgcgggaca
     3901 tcaggatctg caccttctgc tcggcctcct gcagcgcctg ctggcattcc ttggtgaggc
     3961 ggatgccctg ctcgaacgcc tccagcgact tctccagcgt cagttcgccg ctttccattt
     4021 tttccaccag ctgttccagg gtttgcaggg atttttcgaa atccaccgcg gattttttct
     4081 tggccattgg gggcacctcg cgcgaaggcg gaaagtgtcg gtgattgccg tcacgcggtc
     4141 aatccgccgc ggtctgcctg ctagactgaa atcacatgac attatgaaaa aggggaagcg
     4201 cgcttggcat tttcacgcag gatgttgtgc tggctgctgg cgggcggctg cgttctggtg
     4261 ctgtcgctac cggtccgggc aacggaaccg gcaccggctg cgacagggtc gccacctgct
     4321 tccggacgcg cggcggcggt gatgaagcga ctgaccggct ggacccgggc ggatgcggcc
     4381 tggctggcgg cgcagttgca ggcggatatc ggccgtggtt cgctggaagt cgaagcccag
     4441 aaacggcgca tcctggtgcg ggtgctggac cgcgatgcct ttggtgccgg cacggctact
     4501 ctgcggccct cattccagcc ggtaatggac cgggtgcgcg tggccatccg ccagctgcag
     4561 ggcgacattt cggtggaagg ccataccgac gacattccag tgaacaatgc ccgctttcgt
     4621 tccaattggg agctgtctgc ttcgcgggcg gcagcagtgg cccaggatct gctgcaacag
     4681 ggcgaatttg agccgacccg atttcgcgtc gtgggctatg gcaatacccg gcccctggtg
     4741 gccaacgaca cggaagagca ccgcagcctc aaccgcaggg tggaaatcgt gatcgaccag
     4801 gggcgtgatg acgccgccta cctgtcgggc gcaaagcccc tgccggctgc ccagcgaccc
     4861 gcatccgccg ccgacgagct gttctgatgg gttggccccg cgcgtcctga cccggcaggc
     4921 attgccttgc cggagccagc gggttaatgt gcggtcattt agggccacct gaagatcgtc
     4981 atggttgaat ccactctggc cgcgctcgac cgcagcggcg cccatgtcat ttatgcagcc
     5041 ttgctggcgg atctgctggc ggagcggggc attggcgtgg acgccctgct gcacaatacc
     5101 ggcattgcgc cggcggatct caaatccatc gatggccgtc tcagcacgcg ccagtacatt
     5161 gccctgatcc gcaatgcgcg ccggctggcc aatgatcctg cgctggcgtt tgctttcggc
     5221 ctgcacctga aactgtccac ccacggtttt ctcggtttcg ccgccatgag cgcgtccacg
     5281 ttgggcgagg cgctggaact ggcggtgaaa tactgccgca cccgcttcga attcatgcgc
     5341 ctgctgtatt accgccagca gggctttggt gtgctggaga tcgacgaggc cgtgcccatg
     5401 ggcgaagcct acctgttcat gctcgaagcc ttcatgacca gttttgccgt catgagcatg
     5461 aacctgctgg gggaaattcc ccgtgacatc caggtgcgcc gcaccggcga caagccactc
     5521 catttcgatg atgccatgcg ttatttccag caggggcatg cggtgcgctt caacgacacg
     5581 gtgaaccaga tcctgattcc cgaagtgcat ctgcagcgca aactggtgct gtccgacccg
     5641 gtggcgcggc agttggctga agcgcaatgc gagaaggaat tgcagacaat acaggtgcag
     5701 gacgacctgg tgtatcgtgt cacgcggctg ct
//
LOCUS       DASUEG010000229         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2122923, whole genome shotgun sequence.
ACCESSION   DASUEG010000229 DASUEG010000000
VERSION     DASUEG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122923"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1485
                     /gene="ccoG"
                     /locus_tag="VFX11_04340"
     CDS             76..1485
                     /gene="ccoG"
                     /locus_tag="VFX11_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX4937880.1"
                     /translation="MANSGKIAVKEINPGDRNSGDLYKKREHIYQRHISGFFQTLRIA
                     TIWVTLGAYFILPWFTWDGRQGILFDLPERKFYILWFTFWPQDFLYLSWLLIIAAFAL
                     FFFTVLAGRVWCGYTCPQTVWTTFFMWIEFITEGDRSQRIKLDAQPWSVSKGIKKFSK
                     HFLWLALSFWTAFTFVGYFAPVRELWSDIYHFNASAWAWFWLFFFTIATYTNAGWLRE
                     QVCLYMCPYARFQSVMFDRDTLVVTYDKQRGEPRGSRKKDADFKAEGLGSCINCQMCV
                     QVCPTGIDIRNGLQYECIGCAACIDACDHIMDQMNYPRGLIRYSTESIMEGKPYNILR
                     PRLIGYGVMLMAMCLLFLYVLLSRVPLQVDIIRDRSALYRETFEGNIENTYTLKIMNK
                     TQDHQEYTVTASGLDDAIAHIPESIRALPGELVNLPVTLEIAPDKLKQRNTTVYFTVV
                     AKSDPAQEVTEESRFLGPH"
     gene            1526..2023
                     /locus_tag="VFX11_04345"
     CDS             1526..2023
                     /locus_tag="VFX11_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX4937881.1"
                     /translation="MTTPSKPWYKQFWPWFLIAIPGSSVVVGTVMIVLAMQDQGGLVR
                     DDWYKEGMAINERLDQQKKAKTAGIKGFFSFATDENIISLRVENIDAAQEASLTLDLI
                     HPTQKQRDIAVELVRTPQNTYFAKLDHTPAGFYYVKLRSAPGQWEIESQLNFGNTLHD
                     VELAN"
     gene            2068..>2316
                     /locus_tag="VFX11_04350"
     CDS             2068..>2316
                     /locus_tag="VFX11_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEX4937882.1"
                     /translation="MNAPPTGPACFHCGLPVPADCTLQVTIAGTPRAMCCPGCQAVAQ
                     TIVDIGMESYYEHRTASAPGTLQAPELVPEFLNELQDWD"
BASE COUNT          480 a          714 c          639 g          483 t
ORIGIN      
        1 cggtgaccgg gcgcgccttt tttccgccag cagtttctta caccctgatt caacccgtct
       61 gaaagcaggt gacgtatggc aaattccggc aagatcgcgg tcaaggaaat caatcccggt
      121 gaccgcaaca gtggcgatct ctacaagaaa cgcgagcata tttaccaacg ccacatctcc
      181 ggttttttcc agacactgcg catcgcgacc atctgggtca cccttggcgc ctacttcatt
      241 ctgccctggt tcacgtggga tgggcgccag ggcattctgt tcgatctgcc tgaacgcaaa
      301 ttctatattc tgtggtttac cttctggccg caggattttc tctacctgtc ctggctgctg
      361 atcatcgcag cgtttgcgct gttctttttc accgtgctgg ccggccgcgt ctggtgcggc
      421 tacacctgtc cgcaaacggt gtggactact ttcttcatgt ggatcgaatt cattaccgaa
      481 ggcgaccgca gccagcgcat caagctggac gcacagccct ggtctgtctc caagggcatc
      541 aagaaattca gcaagcattt cctgtggctg gcgctgtcgt tctggacggc gttcaccttc
      601 gtcggttact tcgccccggt acgcgaactc tggtccgaca tttaccattt caatgcatcg
      661 gcgtgggcct ggttctggct gttcttcttc accatcgcca cctacaccaa cgccggctgg
      721 ctgcgggagc aggtgtgtct gtacatgtgc ccctatgccc gcttccagag tgtgatgttt
      781 gaccgcgaca ccctggtggt aacctacgac aagcaacgtg gcgaacctcg cggctcgcgc
      841 aaaaaggatg ccgatttcaa agccgagggc ctgggttcct gcatcaattg ccagatgtgc
      901 gtgcaggtgt gtcccaccgg tatcgacatc cgcaatggtc tgcagtacga atgcatcggc
      961 tgcgccgcct gcatcgacgc ctgcgatcac atcatggacc agatgaatta cccgcgcggc
     1021 ctgatccgtt attccaccga aagcatcatg gagggcaagc cgtacaacat cctgcgaccg
     1081 cgcctgatcg ggtacggcgt gatgctgatg gccatgtgcc tgctgttcct gtatgtgctg
     1141 ttgtcccgcg tgccgctgca ggtggacatc atccgcgacc gcagtgcgct gtaccgcgag
     1201 acgttcgaag gcaacattga aaacacctat acgctgaaga tcatgaacaa gacgcaggac
     1261 catcaggaat acaccgtgac ggcgtccggc ctggatgatg cgattgccca tattcccgag
     1321 tcgatccgcg ccctgcccgg cgagctggtg aatctgccgg taacgctgga aatcgcgcca
     1381 gacaaactga agcagcgcaa taccaccgtg tacttcaccg tggtggcaaa atccgatccg
     1441 gcgcaggaag tgacggaaga aagccggttc ctcggtcctc actgaccgtt gccggaatgc
     1501 acccgattca cttgcgagca cgagcatgac cacaccaagc aaaccctggt acaaacagtt
     1561 ctggccctgg ttcctgatcg ccatccccgg cagctcggtg gtggtgggaa ctgtgatgat
     1621 cgtgctggca atgcaggatc agggcggtct ggtgcgcgat gactggtaca aggaaggcat
     1681 ggcgatcaac gagcgcctgg accagcagaa aaaggcgaaa actgcgggca tcaagggctt
     1741 cttcagtttt gccacagatg aaaacatcat ttccctgcgg gtggaaaata tcgacgccgc
     1801 gcaggaagcc agccttacgc tggatctaat ccaccctacc cagaaacagc gtgacattgc
     1861 ggtggagctg gtgcgcacgc cgcagaatac ctacttcgcc aaactggacc acacaccggc
     1921 cggtttctac tacgtcaagt tgcgctcggc gcccgggcag tgggaaatcg aaagccagct
     1981 gaacttcggc aacaccctgc acgatgtcga gctggcgaac tgagcctgct cccatgaaca
     2041 cctcaccgcc atgatagcct tgctgccatg aacgccccgc cgaccggtcc tgcctgtttc
     2101 cactgcggcc tgcccgttcc cgccgactgc acgctgcagg tcaccattgc cggcaccccc
     2161 cgcgccatgt gctgtcccgg ctgccaggcg gtggcgcaga ccatcgtcga catcggcatg
     2221 gagagctatt acgaacaccg gaccgccagc gcgccaggca cattgcaggc gccggaactg
     2281 gtgccggaat ttctcaatga attgcaggac tgggat
//
LOCUS       DASUEG010000230         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1581381, whole genome shotgun sequence.
ACCESSION   DASUEG010000230 DASUEG010000000
VERSION     DASUEG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581381"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFX11_04355"
     CDS             complement(<1..550)
                     /locus_tag="VFX11_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX4937883.1"
                     /translation="MKAGVTVNRNTLITVIILTAAVLALGVYLYQHLERYEKHTNTGP
                     SLKVLLNPWFAAQRFLERHNIESHRSHDLHSILGRLKPDDTLVLFNDTAIYSTSNRAR
                     LEEWMQSGGHLIIAANYEWDEEEESSGDPFLDAYGVRMSWTEEDEEAEEEDEDTYEEE
                     ATENDAATADEAETAIAESDIAE"
     gene            complement(547..2148)
                     /locus_tag="VFX11_04360"
     CDS             complement(547..2148)
                     /locus_tag="VFX11_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX4937884.1"
                     /translation="MDLSKITARVRPRNPYEAADLGIVMARQWFRPLMLLWLLPSLPL
                     MLVLYGIFHAQPLWALLILWWLKPLFETLQLQFIAEALFNPDARWQDFAKKLPTLLRH
                     QLLAKLFVQRWSLSRSFNMPVGELERLGGSQRARRLSTLHRGANAASIWLTLLGTHLE
                     ALLVSALFLAAWMLVPVEMSVDFEIDDLLDSPLLFPTLAWGGYLAMWIIAPFYVCCGF
                     MLYINRRTWLEAWDIELTFRQLAARHAAPHTAPHATPTRTLPVLLLGTLLLLPLAQPS
                     PVQAESSAPAQTEAAAPTPPAPLTPEQSLQEISDILESDDFNQIKTRQSLRWADDSAR
                     RKAELKGEDKGLLSDLGSWLDDFFKNSPGFKTTLDWLAGLPELLEILLWGLVIALFVI
                     LIYRFRDFAIPGLRRQTKTARTIPTHLFGLELDQTSLPDDLIAAARSLWQQQRPREAL
                     GLLYRGALSFLVFECQLPLEDSHTEDECVTLCGRREHPARADYFRRLTAHWVRLAYAH
                     QTLGEADFTLLCEQWPDFTHSGEAA"
     gene            complement(2138..>2625)
                     /locus_tag="VFX11_04365"
     CDS             complement(2138..>2625)
                     /locus_tag="VFX11_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX4937885.1"
                     /translation="DDFVMFGFYIKNNIGIGFQTFASGILLGIGALFHLIFNGVAIGT
                     VAGYLTELGHTETFWPFVVGHGAFELTGIAISGAAGLKLGYGLLCPGRLTRRQSLINA
                     AREAMPLVYGVFFLLLIAAFVEAFWSSSTNLPDTAKYVAGASFWLLVISYLCLGVRRG
                     S"
BASE COUNT          497 a          815 c          822 g          491 t
ORIGIN      
        1 actcagcaat gtcggactca gcaatggcag tctcagcctc gtccgcagtg gcagcgtcgt
       61 tttccgttgc ctcttcctcg tacgtgtcct cgtcctcttc ttctgcttcc tcgtcctctt
      121 cggtccagct catgcgcacg ccataggcgt ccaggaacgg gtcgccgctg gattcttcct
      181 cttcatccca ttcgtagttg gcggcgatga tcaggtggcc gccagactgc atccactcct
      241 ccagccgcgc gcggttgctg gtgctgtaga tcgcggtgtc gttgaacagc accagggtgt
      301 cgtccggttt cagccggccc aggatgctgt gcagatcgtg cgagcggtgg gattcgatat
      361 tgtgccgctc cagaaaacgc tgggcggcga accaggggtt cagcagcact ttcaggctgg
      421 ggccggtgtt ggtgtgtttc tcgtagcgct ccaggtgctg gtacaggtag acgcccagtg
      481 ccagcaccgc cgctgtcagg atgatcacgg tgatcagcgt gttgcgattg acggtgactc
      541 ccgctttcat gccgcctccc cgctgtgggt gaagtcgggc cactgctcgc acagcagtgt
      601 gaaatcggct tcgcccaggg tctggtgggc ataggccagg cgaacccagt gggcggtcaa
      661 ccggcggaaa taatcagcgc gcgccggatg ttccctgcgg ccgcacaggg tcacgcattc
      721 atcttcggtg tggctgtctt ccagcggcag ctgacattca aacaccagga acgacagcgc
      781 gccccggtac agcaggccca gcgcttcccg tggccgctgt tgttgccaca gtgagcgggc
      841 ggcggcgatc aggtcgtccg gcaggctggt ctggtccagt tccagcccga acaggtgagt
      901 gggaatcgtg cgtgccgttt ttgtctgccg gcgcaggccg ggaatggcga aatcccggaa
      961 tcggtagatc aggatgacga acagcgcgat caccagcccc cacagcagga tctccagcag
     1021 ttccggcagg ccggccagcc agtccagcgt ggttttgaaa ccgggtgagt tcttgaaaaa
     1081 atcgtccagc cagctgccca gatcgctgag caggcccttg tcctcgccct tgagttcggc
     1141 tttgcgtctg gcgctgtcgt cggcccagcg cagggattgc cgggttttga tctggttgaa
     1201 atcgtcgctt tccagaatgt cgctgatttc ctgcaggctc tgttctgggg tgagtggcgc
     1261 gggcggcgtg ggcgcggctg cttccgtctg tgcgggtgcg gatgactccg cctgcacggg
     1321 ggagggttgc gccagcggca gcagcagaag cgtgcccagc agcaataccg gcagcgtgcg
     1381 tgtcggtgtt gcatgtgggg cggtgtgggg ggccgcgtgg cgcgcggcca gctggcggaa
     1441 ggtgagttcg atgtcccagg cttccagcca ggtgcgacgg ttgatgtaca gcatgaaacc
     1501 gcagcagacg tagaacggcg cgatgatcca catggccaga taaccgcccc aggccagagt
     1561 gggaaacagc agcgggctgt ccagcagatc gtcaatttcg aaatccaccg acatttccac
     1621 cggcaccagc atccatgcgg ccaggaacag tgcgctcacc agcagggctt ccagatgggt
     1681 gcccagcagc gtcagccaaa tgctggccgc attggcgccc cggtgcagcg tgctcaaccg
     1741 gcgtgcgcgc tggcttccgc ccagccgctc cagttcgccc accggcatgt tgaacgagcg
     1801 cgacaggctc cagcgctgca cgaacagttt tgccagcagc tggtgccgca acagggtggg
     1861 aagctttttc gcgaagtcct gccagcgcgc gtccggattg aacagcgctt cggcgatgaa
     1921 ctgcaattgc agggtttcga acagcggttt cagccaccac aggatcagca gcgcccacag
     1981 aggctgggcg tggaaaatgc cgtacagcac cagcatcaac ggcagggacg gcaacaacca
     2041 cagcagcatc agtggacgga accactgccg cgccatcacg atgcccaggt cggcagcctc
     2101 gtagggattg cgtggccgta cccgcgcggt gatcttgcta agatccacgg cgcaccccca
     2161 ggcacagata gctgatcacc agcagccaga aactggcgcc ggccacatat tttgcagtgt
     2221 ccggcagatt cgtgctcgat gaccagaatg cttccacgaa cgcagcaatc agcagcagga
     2281 aaaacacgcc atacaccagc ggcatcgcct cgcgcgccgc attgatcagc gactgccgcc
     2341 gggtcagccg gcccgggcac agcaagccat aacccagctt caacccggcc gcgccggaaa
     2401 tcgcgatgcc ggtcagttca aacgcgccat gtcccaccac gaatggccag aacgtttcgg
     2461 tatggcccag ttccgtcaga tagccggcca ccgtgccgat cgctacgcca ttgaatatca
     2521 gatgaaacag cgcgccgatc cccagcagaa tgccgctggc aaaggtctgg aaaccgatgc
     2581 cgatattgtt cttgatataa aatccgaaca tcacgaaatc gtcgc
//
LOCUS       DASUEG010000231         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1065217, whole genome shotgun sequence.
ACCESSION   DASUEG010000231 DASUEG010000000
VERSION     DASUEG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065217"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFX11_04370"
     CDS             <1..600
                     /locus_tag="VFX11_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4937886.1"
                     /translation="LLEVMQEKQITIEGKAFPALTPFMVLATQNPIEQEGTYPLPEAE
                     LDRFMLKVQIDYPAEAEEQALVRQVTTGRVQDTLSVDAIETVVKAADIPVLQGITANI
                     RVDDAVFDYAVRIVRTSRTWHSFSHGAGPRASIALIRAARAQALLKQRDFVTPDDVKQ
                     MVLPVMRHRVALTAEMEIEGLKTDQALTQMLQEVEAPRV"
     gene            597..1943
                     /locus_tag="VFX11_04375"
     CDS             597..1943
                     /locus_tag="VFX11_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4937887.1"
                     /translation="MRPTRRAFWLATLWMLIGLLPAMNNWLPKDLAKPELQEQLIQLW
                     QIATAGLLFLLVLDALGVRKVSGLIEVSRQHNDNLALGVWSPVTVRIRHRFKRAVPLQ
                     MFDHFPPDGEADQPHQSARLQPGNGVEFQYKVKINKRGNHRFGPVDLLLDSPMGFWQR
                     RLLAGAPTEARVFPNFAAVAQYAMLAMEQQSAQLGIRQQQRRGEGMEFQQLREFRQGD
                     SLRQIDWNASARQRKLISKEYQDEKDQQILFLVDCGRRMRSQDDALSHLDHALNAMLL
                     MSYAALKQGDAVGLMSFGGQERWLKPVKGVANISKVLHAAYDLEATTRASDYTGAVRS
                     LLTRHSKRSLIVLLSNIRDENTDDLVPALRLLQRKHLVLVANLEEQGIHALLNEKVDG
                     FDQALRHAATRAYLERRNQITRQFNRSGLITVNTTPQHMPVKLVNQYFDIKREGLL"
     gene            2055..>2533
                     /locus_tag="VFX11_04380"
     CDS             2055..>2533
                     /locus_tag="VFX11_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937888.1"
                     /translation="MNVAFRSTLPVILSASLLLTGCSTPVAKKPAGRYQPPAGIKVAP
                     PLQEKPPAQTATPPAAGTPSTPQTVALQPITPAPAIQPAPVAKPLQPTPTPQPAPKPA
                     PQPAVKSAAATTDVPAAAALTPTPTQQPPAPAKPVTPKPSEPVPVAAAKTSPASSVGA
                     "
BASE COUNT          517 a          852 c          756 g          408 t
ORIGIN      
        1 ctgctggaag tgatgcagga aaagcagatc accatcgaag gcaaggcatt tcctgcgcta
       61 acgcccttca tggtgctggc gacgcagaac cccatcgagc aggaaggcac ctacccgctg
      121 ccggaagcgg aactggaccg cttcatgctg aaagtgcaga tcgactaccc cgccgaagcg
      181 gaagaacagg cactggtgcg gcaggtcacc accggccggg tgcaggacac cctgagcgtc
      241 gatgcgattg aaaccgtggt gaaggccgcc gacattccgg tgctgcaggg catcaccgcc
      301 aacatccggg tggacgacgc cgtattcgac tacgccgtgc gcatcgtgcg cacctcgcgc
      361 acctggcaca gtttctcgca cggtgccggc ccccgcgcca gcatcgcgct gatccgtgct
      421 gcccgcgcgc aggcactgct gaaacagcgc gatttcgtca cgcccgacga cgtgaaacag
      481 atggtgctgc cggtaatgcg gcaccgcgtt gccctgacgg cagaaatgga aatcgagggc
      541 ctgaagaccg atcaggcgct gacgcaaatg ctgcaggaag tggaagcccc ccgcgtatga
      601 ggcccacccg ccgcgccttc tggctggcca ccctgtggat gctgatcgga ttactgccgg
      661 caatgaataa ctggctgccg aaggatctgg cgaaaccgga actgcaggag caactgatcc
      721 agctgtggca gatcgccact gccggcttgc tgttcctgct ggtgctggat gcactgggcg
      781 tgcgcaaggt cagcggcctg atcgaagtca gccgccagca caacgacaac ctggccctcg
      841 gcgtgtggtc gccggtcacc gtgcgcatcc gtcatcgctt caaacgcgcg gtgccgctgc
      901 agatgttcga ccacttcccg ccagacggcg aagccgacca gccccaccag tccgcgcgac
      961 tgcaaccggg caacggcgtg gaattccagt acaaggtgaa gatcaacaaa cgcggcaatc
     1021 accgcttcgg cccggtggac ctgctgctgg attcccccat gggcttctgg caacgccgtc
     1081 tgctggcagg tgcacccacc gaagcacggg tgttccccaa tttcgccgcc gtcgcccagt
     1141 acgccatgct ggcgatggag cagcagtcgg cgcaattggg tatccgccag cagcaacgcc
     1201 gtggtgaggg catggagttc cagcagttgc gggaattccg ccagggtgac agcctgcgcc
     1261 agatcgactg gaatgccagc gcccgccagc gcaagttgat ttccaaggaa taccaggacg
     1321 agaaggacca gcagatcctg tttctggtgg actgtggccg tcgcatgcgg tcgcaggatg
     1381 atgcgctcag ccacctggat catgcactga acgcgatgct gctgatgagt tatgccgcgc
     1441 tgaaacaggg cgacgccgtg ggcctgatga gtttcggcgg ccaggagcgc tggctgaaac
     1501 cggtgaaggg cgtggccaat atttcgaaag ttctgcacgc cgcctacgat ctggaagcca
     1561 ccacccgcgc cagcgattac accggtgccg tgcgcagcct gctgacccgg cacagcaagc
     1621 ggtcgctgat cgtgctgctg tcgaacatcc gcgacgagaa caccgacgat ctggtgccgg
     1681 ccctgcgcct gctgcagcgc aagcacctgg tgctggtggc caatctggag gagcaaggta
     1741 ttcacgccct gctcaatgaa aaggtcgacg gcttcgacca ggccctgcgt cacgccgcca
     1801 cccgcgccta cctggaacgg cgcaaccaga tcacccgcca gttcaaccgc agcggcctga
     1861 tcaccgtgaa caccacgccg caacatatgc cggtcaaact ggtgaaccag tatttcgaca
     1921 tcaagcgcga ggggttgctg tagccccttt tgcaggattt gtggaatttt gacttgtata
     1981 atgtccggcg gccgatggac catactgttt tggatcacct tgtccgcctg tccttgaacc
     2041 gggaatttcc gccgatgaac gttgcgttca gatccacact gcccgttata ttgagtgcca
     2101 gcctgctgct gacgggctgc agcacacccg ttgccaagaa gcccgccgga cgctaccagc
     2161 cgccggccgg catcaaggtg gcgcctccgc tgcaggaaaa accgcctgcg caaactgcaa
     2221 cgccaccggc agcgggcacg ccgtccacac cgcagacagt cgcactccaa ccgatcactc
     2281 ctgcgccggc tatacaacct gcgccggtgg caaagccgct gcaaccgaca cccactccgc
     2341 agccggcgcc caaacccgct ccgcaaccgg cggtaaaatc cgcggccgcc accaccgacg
     2401 tccctgcagc agcggcgtta accccgacgc cgacacaaca gccccctgcc ccagccaaac
     2461 ccgtcacacc caagccgtct gaacccgtgc cggttgcagc cgcgaagacc agtccggcca
     2521 gttccgtggg ggc
//
LOCUS       DASUEG010000232         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1310637, whole genome shotgun sequence.
ACCESSION   DASUEG010000232 DASUEG010000000
VERSION     DASUEG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310637"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFX11_04385"
     CDS             <1..336
                     /locus_tag="VFX11_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4937889.1"
                     /translation="MPYLWAIIAKGSAPKFNNSKPREWLSKLEGRGARANWAQQNSFE
                     AMPGFLAAVIIAHLAQAPQHQIDLIAVNFVVLRLFYGYLYLADQATLRSVVWALGVAC
                     VVALFVISV"
     gene            complement(637..1560)
                     /locus_tag="VFX11_04390"
     CDS             complement(637..1560)
                     /locus_tag="VFX11_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEX4937890.1"
                     /translation="MPDPHQGLAPHVALQQIDELTCLRITHPRASALIALQGAQVLEF
                     TPTNTQPLIWLSETACFKRGQSVRGGIPVCWPWFGDLARNPQAVRDTIGQLEPPAPAH
                     GWARSQPWLLQEIGETDDEVKIRLEYPAPMGLPVAWANQVSMTLDISIGQRLHLALTT
                     HNHGNHALTLSQALHSYFAVSHIDQVRILDLENVRYLDTLQQWAQRQNPTPLPINQET
                     DRIYLDTPPLIRIDDAGWQRIVCIESRSSRSAVVWNPWIEKSKRLSQFEADAYQRMLC
                     IETARVMDDCLVVPAGGSSCMEVSIWQEESR"
     gene            1704..>2395
                     /locus_tag="VFX11_04395"
     CDS             1704..>2395
                     /locus_tag="VFX11_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937891.1"
                     /translation="MIHLPRGLRAVALGTTLFTMTGCEYVNDVPLLARFIAKNVCGDI
                     LVSGYDEAAAKSYVANIAPPFKNSWNISRNADSGVVSVRNRWLPTYGTQTALPVNADV
                     GCRNPYAGVSAQVPVALPVPSISLDWPDARAQFPALQQFVQAEVDAGAPTNTTAILVV
                     HDNRIVAEAYRDGIGPDSPLKGFSMSKSLANLFVGRLADQGRLQVTDPMPMAAWQGDA
                     RASIRWEDSLRM"
BASE COUNT          398 a          679 c          784 g          534 t
ORIGIN      
        1 atgccgtatc tgtgggccat tattgccaag ggcagtgcgc ccaaattcaa caacagcaag
       61 ccgcgggagt ggctgagcaa gctggaaggg cgtggcgccc gcgccaactg ggctcagcag
      121 aacagtttcg aagccatgcc cggattcctc gcggcggtga ttattgcgca tctggcgcag
      181 gcgccgcagc accagatcga tctgatcgct gtcaattttg tggtgctgcg cctgttttat
      241 ggttatctct acctggcgga ccaggccacc ctgcgttcgg tggtctgggc actgggtgtg
      301 gcctgcgtgg tggcattgtt cgtgatcagc gtctgatccg tcagtccggg ccggcggcag
      361 gtttctgcgc tggcccggaa caagctgctt atcgattccc gcgttttaat tattttttcc
      421 atttggttca cttcagccgc ggtcggtgcc ggattgtccg caatgcaccg tcatgttttt
      481 tacataaacg ctaagatgga gacgtcatgg ctaagtcgcg ccgtactccg ggccgacgct
      541 ggactccatt aaggattttc gtcatcggcc cggggctccc tctgctccgg gttcttttgg
      601 ccgcgcctcc ggatacccct gggagcgcgg ttttttttag cggctttctt cctgccagat
      661 actcacttcc atgcagctgc tgccaccggc tggcaccacc agacaatcgt ccatcacccg
      721 cgcagtttcg atgcagagca tgcgctggta ggcgtctgct tcgaactggc tcaggcgttt
      781 tgatttttcg atccagggat tccacaccac ggcagaacgg gagctgcggc tttcgatgca
      841 gacgatgcgt tgccagccgg cgtcgtcgat gcggatcagg ggtggcgtgt ccaggtagat
      901 gcggtcggtt tcctggttga ttggcaacgg ggttgggttc tgccgttgcg cccactgctg
      961 cagcgtgtcg agataacgca cgttttccag atcgagaatc cgtacctgat cgatatggct
     1021 gacggcgaaa tagctgtgca gtgcctgcga cagggtgagt gcgtggttgc cgtgattgtg
     1081 tgttgtcagc gcgaggtgca ggcgctggcc gatgctgatg tccagcgtca tgctcacctg
     1141 attggcccag gctaccggca ggcccatggg ggcgggatat tccagccgga tttttacttc
     1201 atcgtcggtt tcgccgattt cctgcaacag ccagggctgg ctgcgggccc agccgtgggc
     1261 gggtgcgggc ggttccagtt gtccaatggt gtcgcgcacc gcttgcggat tgcgggccag
     1321 atcgccgaac cagggccagc acaccggaat gccgccgcgc actgactggc cgcgcttgaa
     1381 gcaggcggtt tcgctcagcc agatcagcgg ttgcgtgttg gtgggggtga attccagcac
     1441 ctgggcgcct tgcagcgcga tcagggcgct ggcgcggggg tgggtgatgc gcaggcaggt
     1501 cagctcgtcg atctgctgca gcgcgacatg gggcgcgagg ccctgatgcg ggtccggcat
     1561 ggggatttcc tttcggcgta tggccaggca agtggactgt taaaggcgcc agtgtgccca
     1621 aaggcgggag cggtgaaaag ctgcctgttt tctgctagcg tgggttggct gaaaaaccca
     1681 ataataaatt cgagggaacg aacatgattc acttgccacg ggggctgcgc gctgttgcgc
     1741 tgggcacgac actgttcacc atgaccggct gcgagtatgt caatgatgtg ccgctgctgg
     1801 cccgttttat tgccaagaac gtctgcggcg acatcctggt gtcgggctat gacgaggccg
     1861 ctgccaaatc ctatgttgcc aacatcgcgc cgccattcaa aaacagctgg aacatcagcc
     1921 gtaatgccga tagcggtgtg gtcagcgtgc gtaaccgctg gttgccgacc tatggcaccc
     1981 agacagcgtt gccggtaaac gctgacgttg gctgccgcaa tccttacgcc ggcgtctctg
     2041 cccaggtgcc ggtggcattg ccggtacctt ccatcagcct ggactggcct gacgcccgcg
     2101 cgcaattccc tgcgctgcag cagttcgtgc aggccgaggt ggatgccggc gcgcccacca
     2161 acaccaccgc gattctggtg gtgcatgaca accggatcgt ggcggaagcc tatcgtgacg
     2221 gtattggccc ggattcgccg ctgaaaggtt tttccatgtc gaaaagcctg gccaatcttt
     2281 tcgtgggtcg gctggcggac caggggcggt tgcaggtgac ggatccgatg ccgatggcgg
     2341 cctggcaggg ggatgcgcgt gcctccatcc gctgggagga cagcctgcgc atgag
//
LOCUS       DASUEG010000233         4779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_910561, whole genome shotgun sequence.
ACCESSION   DASUEG010000233 DASUEG010000000
VERSION     DASUEG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910561"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFX11_04400"
     CDS             <1..1726
                     /locus_tag="VFX11_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4937892.1"
                     /translation="AHPIITNSEELRHHHQTFYRYTARPLGEILLDMEAITPAQLSDA
                     LAEQKISHKRLGEILQQLGLATREQVLRALSERFGVPFINLRQFDIDPKALPCVPAAF
                     ARKHMVIPVLLDNDRLLVAMSDPTDTELINMLRFLTGKVLEFCIAPGDDIAYAITTYY
                     GVQEVQTALDGMTVLSRDEPFHVDANSEEQLGNERPVVQLVQTVIADAMVREASDIHI
                     RPREGVVDLLFRVDGVLQRIRTFSKKLLPAVVSRIKIIGNMDISEHRLPQDGRSRIHY
                     LDKHVDLRLSVIPTIYGESVVIRLLDTQFALKNLEQLGFDVADALKLRHLLTRTSGIF
                     LVTGPTGSGKSTTLYTALDHVKASNVNIITVEDPVEYHMDGIAQIQVNHSTGYSFARA
                     LRHILRHDPDVIMVGEIRDQETAKMAVESALTGHLVLSTLHTNDAATSVTRLIEIGVE
                     PYLVNSSLIGVLAQRLARCNCPHCKAPEEIDPEIRRVLGVGIDEVFYVGKGCERCHQT
                     GVKGRMAVYELLEVTPELRKLVMQSVSADVIERQAVNDGMTPLTQNALKAARAGRLSL
                     AEVYRVRL"
     gene            1903..2733
                     /locus_tag="VFX11_04405"
     CDS             1903..2733
                     /locus_tag="VFX11_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4937893.1"
                     /translation="MLQFLTLRFPAVSSERWHDRMSRGDVFDARGQPIPPDSPYLPGT
                     RLYYYRELETELPVPFPHRILHEDDHLLVADKPHFLPTVPSGGYLHETLISRLRHERG
                     QGDLEVLHRLDRDTAGLVLIAKHPGARDAYHRLFRERQVDKVYLAMAPLPEALEFPLT
                     RASHIGAAADFYRRQEGAGEVNAITRIELAAQRQEGLGLYRLFPVTGKTHQLRIHMAA
                     LGMPILNDPYYPAETPREPGDFSQPLQLLAQALVFVDPFTGAERQFTSTFTLAQDFSD
                     "
     gene            complement(2758..3585)
                     /locus_tag="VFX11_04410"
     CDS             complement(2758..3585)
                     /locus_tag="VFX11_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX4937894.1"
                     /translation="MQIEPFFQVPQHPHATSAGDVQLPLLYKEADAVLTLFLCDLDRV
                     RQQLEGTGLEPALVVGRRAIVTMAIYDFIHCNIGAYHTLPVSIPVWRDQGFRPRSAWR
                     ELFLPADKRHMGFYMLSAPTSSNASCTAGRELWGFPKHLVEIDFKLQGTRVACRMSAG
                     HDSLLEFGGRGVPLSWIMQLDINLFSIKQDLLLRTLLNTRGRFRAHFPFGYQLRLGST
                     DHPATRPLRALGMDRKRPLALITCADYQGRMQEGVAVERLHRPVAPRPGPNPSHLMA"
     gene            3910..>4779
                     /locus_tag="VFX11_04415"
     CDS             3910..>4779
                     /locus_tag="VFX11_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX4937895.1"
                     /translation="MRIAQEALTFDDVLLIPGYSDILPRDVELKTRLTRDITLNIPLI
                     SAAMDTVTESRLAIAMAQEGGIGILHKNMTEEEQARQVRLVKKYESGVVRDPITINPE
                     ATIGQLLDLTNAHKISGVPVVKGQELVGIVTSRDLRFENRYELPVSSIMTPKDKLVTA
                     KEGASTDEIKSLLHKYRIEKVLLVDDNFHLKGMVTNTDILKAEAYPNGCKDNQGRLRV
                     GAAVGTGAGTEERVAALVEAGVDVLVVDTAHGHSQGVLSRVKWVKDNFPQVQVIGGNI
                     ATAAAGLALADHGA"
BASE COUNT          933 a         1481 c         1488 g          877 t
ORIGIN      
        1 ggctcacccc atcatcacca actcggaaga gttgcgccat caccaccaga ccttctatcg
       61 ctacaccgcc cgcccgctgg gtgaaatcct gctggacatg gaggcgatta cgcccgccca
      121 gctgagcgat gccctggccg agcagaagat cagtcacaag cggctgggcg aaatcctgca
      181 gcagctgggg ctggcgaccc gcgagcaagt gttgcgggcc ctcagcgagc gcttcggcgt
      241 gcctttcatc aacctgcgtc agtttgatat cgatcccaag gcattgccct gtgtgccagc
      301 ggcatttgcc cgcaagcaca tggtgattcc ggtgctgctg gacaacgacc gcctgctggt
      361 ggccatgtcc gatcccaccg acaccgaact catcaacatg ctgcgcttcc tcaccggcaa
      421 ggtgctggag ttctgcatcg cgcccggtga cgatatcgcc tacgccatca ccacctatta
      481 cggcgtgcag gaagtgcaga ccgccctcga cggcatgacc gtactgtcgc gggatgaacc
      541 ctttcatgtc gacgccaaca gtgaggagca gctgggcaat gaacgcccgg tggtgcaact
      601 ggtgcagacg gtaattgccg acgccatggt gcgcgaggca tccgacatcc acatccgccc
      661 ccgcgaaggc gtggtggacc tgctgttccg cgtcgatggc gtgttgcagc gcatccgcac
      721 cttcagcaag aaactgctgc cggcggtggt gagccgtatc aagatcatcg gcaacatgga
      781 tatttccgaa caccgcctgc cccaggacgg ccgctcccgc atccactacc tcgacaagca
      841 tgtggacctg cgtctgtcgg tgattcccac catttacggt gaaagcgtgg tgatccgtct
      901 gctggacaca cagttcgcgc tgaagaatct ggagcagctc ggtttcgatg tggcggacgc
      961 gttgaaactg cgtcatctgc tcactcgcac cagcggcata ttcctcgtca ccggccccac
     1021 cggttccggc aaatccacca cgctgtacac ggcgctcgat catgtgaagg cgagcaacgt
     1081 caacatcatc accgtggaag atccggtgga ataccacatg gacggcattg cccagatcca
     1141 ggtgaaccac tccaccggct acagttttgc gcgcgcgttg cggcatatcc tgcgccacga
     1201 tcccgatgtg atcatggtgg gcgagatccg cgatcaggaa actgcgaaaa tggcggtgga
     1261 aagcgcgctc accggccacc tggtgctgag caccctgcac accaacgacg ccgccacgtc
     1321 cgtcacgcgg ctgattgaaa tcggcgtgga gccctatctg gtgaacagtt cgctgattgg
     1381 cgtgctggcg caacggctgg cgcgctgcaa ctgcccccac tgcaaggcgc cggaagagat
     1441 cgatcccgag atccgccgcg tgctgggggt gggcatcgac gaagtgtttt atgtcggcaa
     1501 gggttgcgaa cgctgccacc agaccggtgt gaaagggcgc atggccgtgt acgaattgct
     1561 ggaggtgacg ccggaactgc gcaagctggt gatgcaaagc gtgtcggcgg acgtgattga
     1621 gcggcaggca gttaatgacg gcatgacgcc gctcacccag aacgcgctga aagcggcgag
     1681 ggcgggccgg ttgtcgctgg cggaagtgta tcgggtgcgg ctgtaatcgc ggcgtgggtt
     1741 gcgggataat gccggtccgg tgccggtcag gcatttcccg aaggcccaac agatcctgtg
     1801 cgaaagaaac gctccgctcc cgtgctgccg gttcgtgccg gtataagccc caattcggtt
     1861 tacctgccgg cacctgagcc ggacggcccg gcctgtacct ctgtgctcca atttctgacg
     1921 ctccgttttc ccgccgtatc ctccgagcgc tggcacgatc gcatgtcgcg tggcgatgtg
     1981 ttcgacgccc ggggtcaacc catcccgccg gattcccctt acctgcccgg cacccgcctg
     2041 tattactacc gcgaactgga aactgaactt ccggtgccgt tcccccatag aatcctgcat
     2101 gaggacgatc acctgctggt ggcagacaaa ccgcatttcc tgcccacggt gccctccggc
     2161 ggctacctgc acgaaaccct gatcagccgg ttgcggcacg agcgggggca gggcgatctg
     2221 gaagtgctgc accggctgga ccgggatacc gccgggctgg tgctgatcgc caagcatccg
     2281 ggcgcacggg acgcctacca ccggctgttc cgcgagcggc aggtggacaa ggtttatctg
     2341 gctatggcac cgttacctga agcgctggaa ttccccctga cccgcgccag ccacatcggc
     2401 gccgctgccg atttctaccg ccggcaggaa ggggcgggag aggtgaatgc catcacccgc
     2461 attgagcttg ccgcacagcg gcaggaaggc ctgggtttgt accggctgtt cccggtcacc
     2521 ggcaagaccc accagctgcg tattcacatg gcggcgctgg gcatgccgat cctgaacgat
     2581 ccctactatc ccgctgaaac cccgcgtgag cctggcgatt tcagccagcc cctgcaattg
     2641 ctggcgcagg ccctggtgtt tgtcgaccca tttaccggcg cggagcgtca gttcaccagt
     2701 acgtttacgc tggcgcagga tttttccgat tgagcgcatg ttcctgtcgc caggatttca
     2761 ggccatcagg tgggatgggt tagggcccgg acgcggggcc actggccggt gcagtcgctc
     2821 caccgccacg ccttcctgca tgcgaccctg gtaatcggcg caggtgatca gggccagcgg
     2881 gcgtttgcgg tccatgccca gtgcccgcag ggggcgggta gcgggatgat cggtgctgcc
     2941 cagccgcagc tggtagccga aagggaagtg ggcgcggaag cggccccggg tattgagcag
     3001 ggtacgcagc agcagatcct gcttgatgga aaacaggttg atatccagtt gcatgatcca
     3061 gctcagcgga acgccgcggc cgccgaattc cagcagcgaa tcgtgtccgg ctgacatccg
     3121 gcaggcgacg cgggtgccct gcagtttgaa atcgatctcc accaggtgct tggggaatcc
     3181 ccacagttcc cgtccggcgg tacagctggc gttgctgctc gtgggcgccg agagcatata
     3241 gaaccccatg tggcgcttgt ccgccggcag gaacagctca cgccaggcgg acctggggcg
     3301 aaagccctgg tcacgccaca cgggaatcga gaccggcagg gtgtggtagg cgccgatgtt
     3361 gcagtggatg aagtcataga tcgccatcgt cacgatggcc cgacggccca ccaccagcgc
     3421 cggttccagc ccggttccct ccaattgctg acgcacccgg tccaggtcac acaggaacag
     3481 ggtcagcacc gcatcggctt ccttgtacag caatggcagc tgcacatcgc cggcgctggt
     3541 ggcgtggggg tgttgcggta cctgaaaaaa cggttcgatt tgcatggctt ccctcgcgaa
     3601 gtaccggcgg aacaggcagg ccggcgcagg tgtgataccc atcagtcaca gttgtctggg
     3661 cagtcgatct tagcaatgaa tcaggtggct taattgttta atccggtcaa ctttgcggca
     3721 gacaggtgcc gggtgcctgg aaatggctaa cgcagccttg atccagggcc ggatttccgg
     3781 ctggccggac acttaccgtt acggggtggc gctttgtatc gacgagcagc tttatttcaa
     3841 acagacacgc agctttgtta taattccgcg tttaaccgcc ctcccccctc ccacaggcag
     3901 gtgcccatgc tgcgaatcgc ccaggaagcc cttacgttcg atgatgtttt gctgatcccc
     3961 ggctactccg acatcctgcc ccgcgacgtc gaactgaaaa cccggctgac ccgcgatatc
     4021 accctgaata tcccgctgat ttccgctgcc atggatacgg tgaccgaatc ccggctggcc
     4081 attgcgatgg cccaggaagg ggggatcggt atcctgcaca agaacatgac ggaagaagag
     4141 caggcccgtc aggtgcggct ggtgaagaaa tacgaaagcg gcgtggtgcg cgatccgatc
     4201 accatcaatc cggaagccac catcggccag ctgctcgatc tgaccaacgc ccacaagatc
     4261 tccggtgtgc cggtggtgaa ggggcaggaa ctggtgggca tcgtgaccag ccgtgacctg
     4321 cgctttgaaa accgctatga actgccggtg tccagcatca tgacacccaa ggacaagctg
     4381 gtaaccgcca aagagggcgc cagcaccgac gaaatcaaat ccctgctgca caaataccgc
     4441 atcgagaaag tcctgctggt ggatgacaac ttccacctga aaggcatggt caccaacacc
     4501 gacatcctga aagcagaagc ctatcccaat ggttgcaagg acaatcaggg ccgcctgcgc
     4561 gtgggcgcag cggtgggcac tggcgccggc accgaagaac gggtggcggc actggtggaa
     4621 gctggcgtgg atgtgctggt ggtggatacc gcccacggtc attcccaggg cgtgctgagc
     4681 cgggtgaagt gggtgaagga taacttcccg caggtgcagg tgatcggggg caacattgcc
     4741 accgcagcgg cgggtctggc gctggcggat catggcgcg
//
LOCUS       DASUEG010000234         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1700424, whole genome shotgun sequence.
ACCESSION   DASUEG010000234 DASUEG010000000
VERSION     DASUEG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700424"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1417
                     /locus_tag="VFX11_04420"
     CDS             35..1417
                     /locus_tag="VFX11_04420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4937896.1"
                     /translation="MVFDRFSVFFMALILGASAVITVFCYSYFNGREGLQEELYILLL
                     TATLGALVLVSSTHFASFFLGLEILSVSLFAMVAYPVTTLTRSEGESANPLEAGIKYL
                     ILSGISSAILALGMAFVYAATGAMDFSGIDKVLSHSAYNIYSIAGIAMMIAGVGFKLS
                     LVPFHMWTPDVYQGAPAPVTAFLSTVSKSAVLALILRGFIALDGYRYDSVLLGISVIA
                     IASMLIGNLLALRQNNIKRLLAYSSISHSGYILVAFLAAMSVSMHTSGQADPIALEAV
                     TYYIVTYVLTTLTAFGVVSVLSSSNHIGGETRDVDRFEDYTSLFWRRPVVATVFTAAL
                     LSSAGIPLTIGFIGKFYIFAAGVNGALWSLLLVLIVSSGIGLFYYLRLILVMFKSESD
                     HSPDIVGGGVTGNGVTDIDVLTAGSETRPLGSRWLIALLGALILFLGIYPSPLMAVIR
                     EVVQSMGVGG"
     gene            complement(1438..>2219)
                     /locus_tag="VFX11_04425"
     CDS             complement(1438..>2219)
                     /locus_tag="VFX11_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4937897.1"
                     /translation="FSQIAGDAFLDWGWRLPFALSAILVGVGLWVRLAVVETPVFQRL
                     VAENKIERTPMLEVLRKQPREILLTSLARLGEQTPFYIFTAFIFAYGTGELQMSRDLL
                     LTAVLTAACVSFVSVPAFGHLSDRIGRRKTYLIGLVLTGIFGFIYFALLDTRMPALIF
                     LAIVLSLIPHDMLYGPQAAMIAESFTPRLRYSGASLGYQLASIIAGGPAPLIATWLFG
                     VYKTGYAIAGYILCCVAISLIATLALKDHTNKDIADEYDAP"
BASE COUNT          459 a          577 c          635 g          548 t
ORIGIN      
        1 ggccgacatt ccacccagtg aagtcacacc cctgatggtg ttcgaccgct tttccgtctt
       61 tttcatggcg ttgattctgg gcgcgagcgc cgtcatcacc gtattttgct acagctattt
      121 caatggacgg gagggcttgc aggaggaact ttacatcttg ctgttgaccg caacgctggg
      181 tgcgttggtg ctggtatcca gcacgcactt cgcgtcgttc tttctggggc tggaaatact
      241 cagtgtttcg ctgtttgcga tggtggccta ccccgtaacc acgttgacgc gcagtgaagg
      301 cgagtcggcc aaccccctgg aagccggtat caaatatctc atattgtccg gcatatcctc
      361 cgccattctg gcgctgggta tggcctttgt gtacgcagcg accggtgcaa tggatttcag
      421 cggtatcgac aaagtgcttt cccactccgc atacaatatt tattcaatcg ccggaatcgc
      481 catgatgatt gccggggtgg gattcaaact gtcgctcgtg ccatttcata tgtggacacc
      541 cgatgtgtac cagggagcgc ccgcaccggt tacagcgttt ttatccactg tttccaaaag
      601 tgcagtgctg gcgttgattc tgcgcggttt catcgcgctg gatggctatc gttatgacag
      661 cgttctgctg ggcatcagtg tgattgcaat tgcctccatg ttgatcggca atctgcttgc
      721 gttgcggcaa aacaatatca agcgcctgct ggcgtattcc tcgatttcgc actcgggcta
      781 cattctggtg gcttttctgg cggccatgtc ggtgtccatg cacacttcag gtcaggctga
      841 tcccatcgcg ctggaagccg ttacctatta catcgtcacc tacgtgctga caacgttgac
      901 ggcatttggt gtggtgtcgg ttctgtcatc gtcaaaccat attggaggag aaacgcgcga
      961 tgtcgaccgg tttgaagact acacgtccct gttctggcgt cgaccggtgg tggccactgt
     1021 ctttaccgcc gcactgctgt catctgccgg tatcccgctg acgattggtt tcattgggaa
     1081 attctatatt tttgcagcag gagtcaatgg tgcattgtgg tcgctgttgc tggtgctgat
     1141 tgtgagcagc gggattggat tgttttacta tctgcgcctg atactggtca tgttcaaaag
     1201 tgaatccgac cattcgccag atattgtcgg aggcggagtg acgggcaacg gggtgacgga
     1261 tattgacgtg cttactgctg gcagtgaaac ccggcctctg ggcagtcgct ggttgatagc
     1321 attgctgggc gctttgatct tgtttctggg aatttatccc agtccattga tggcggtcat
     1381 tcgcgaagtt gtgcaatcga tgggtgtggg tgggtgaaac gcgccgtaac gctatcgtca
     1441 cggcgcgtcg tattcgtcgg caatatcctt gttggtgtga tctttcaacg ccagggttgc
     1501 gataagactg atcgccacac agcacagaat gtatccggcg attgcgtagc ctgttttgta
     1561 cacaccgaac agccaggtgg caatcagcgg cgctggtccg cccgcaatga tggatgccag
     1621 ttgatagccc agcgaagcgc cgctgtagcg caggcgaggg gtaaaggatt cggcgatcat
     1681 ggcagcctga gggccataca gcatgtcgtg gggaattagt gacagcacaa tggcaagaaa
     1741 tatcagcgca ggcattctgg tgtcgagcag ggcgaaatag ataaatccaa agatgccggt
     1801 tagcaccagt ccgatcaaat aggtcttgcg gcggccaatg cgatccgaaa gatgcccgaa
     1861 cgctggtacc gacacaaacg agacgcacgc ggccgtcaat actgccgtca gcagcaggtc
     1921 gcgcgacatc tgcaattcac ctgtaccata ggcaaatatg aaagcggtga agatgtagaa
     1981 cggtgtttgc tcccccaagc gggccagaga agtgagcagg atctccctgg gttgcttgcg
     2041 aagcacttcc agcatgggtg tgcgttcgat tttattctcg gccaccagcc gctgaaatac
     2101 gggcgtttcc actactgcaa gccgcaccca gaggcccacg cccactaaaa tggcgctgag
     2161 ggcaaacggc agccgccaac cccaatccag aaaggcatcg cccgcaatct ggctgaaac
//
LOCUS       DASUEG010000235         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2058079, whole genome shotgun sequence.
ACCESSION   DASUEG010000235 DASUEG010000000
VERSION     DASUEG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058079"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1782)
                     /locus_tag="VFX11_04430"
     CDS             complement(46..1782)
                     /locus_tag="VFX11_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4937898.1"
                     /translation="MPKQYRVVSQAAPVKGKMPQDVIQGLARIGMKAEQAAMLLQKPL
                     VIKKDLDNPKALAYLEKFTEAGLLVKIEAYEVAAPPPVNAARSQADEVFTLLESTFAQ
                     PIAPTTLTRDYQKSFAIAAATSLVSPLIYVGLILLSVWALVWYFSSGHGLLFGGIEWH
                     GKFLLFLGALSWVVPAVAGSVLILFLLYPLWPQGKPPKPYVLDRKKHARFYRLIEKMT
                     AAIGVPAPQFIEVIPDMNAAAGPVHGMASLAKGELKLVIGMSLVMGTSVQQFMGVLAH
                     EFGHFSQRSSMMAYIWINTVSHWLWQCGYGQDVWTERLERWQEQFDNEIAQVSLFVTD
                     WLLKLVRLLFQNLYRLNVRLTNAMSQQMEFDADLYEARVVGSAQFRAGTLALRRMGYA
                     WSQARQTTFQGLHEQDKMLRNLPAATLEIANGFSPGLLKKIEDDLQVEETRHWDTHPA
                     DLERIANAENAGERGLLQCELPASVLFVEVEKLCETATLNWYLKLGISGAREFMVDNA
                     VLLQAARSPAGPVSTPAKPAASGARDTAGPAPAPALSAHGSDVAAEPKSAPNDSGMIE
                     WTGARLDQKNAD"
     gene            complement(1801..2760)
                     /gene="pip"
                     /locus_tag="VFX11_04435"
     CDS             complement(1801..2760)
                     /gene="pip"
                     /locus_tag="VFX11_04435"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX4937899.1"
                     /translation="MQVLYPEIKPYREHKINVDAPHVLHVEECGNPDGIPVLFLHGGP
                     GAGCESYHRRFFDPQKYRIVLFDQRGCGRSTPHGCLELNTTQHLVADIETIRTQLQVD
                     DWVLFGGSWGSTLALVYAQTHPERVRGMIVRGIFLSRQKDLDWFYKDGANRIFPDAWT
                     DLIKPVRENMRNDLIAAYDKLLHSNNDLQRMSAAKAWSAWEGHCATLRPNNNVLDHFT
                     DTHTAVSLALIEVHYFKHHSFLEENQILRNMDRIKHVPGMIVHGRYDMICPLDNATDL
                     HHEWVNSELQIIRDAGHSAAEAGIIDALVRATDEMARKVGESA"
BASE COUNT          585 a          911 c          853 g          622 t
ORIGIN      
        1 tgtcgtctcg cggattgcga tgacagggcg tcgcgcatcc gcgcgctaat cggcgttttt
       61 ctggtcgaga cgggcgcccg tccattcaat cataccgctg tcattgggtg cggatttggg
      121 ctcggcggcc acgtcactgc catgagccga caaggccggt gccggggcgg gacctgcagt
      181 gtcacgcgca ccggaagccg ccggcttcgc tggagtcgaa acaggtcctg cgggtgacct
      241 ggctgcctgc agcaataccg cattgtccac catgaattcc cgtgcgccgc tgatgcccag
      301 tttcagatac cagttcaggg tggctgtctc gcacagtttt tccacctcga cgaacaacac
      361 cgatgccggc agttcgcatt gcagcaggcc gcgttcgcct gcattttccg cgttggcaat
      421 gcgttccaaa tctgccggat gggtatccca gtggcgggtt tcctccacct gcagatcatc
      481 ttcgattttt ttcagcaggc ccgggctgaa accgttggcg atctccagtg tcgctgccgg
      541 caggttgcgc agcattttgt cctgttcatg caggccctgg aacgtggtct ggcgcgcctg
      601 actccaggca tatcccatcc ggcgcagcgc cagcgtgccc gcgcgaaact gcgcactgcc
      661 caccacccgc gcctcataaa gatcggcatc aaattccatc tgctgcgaca tggcattggt
      721 caggcgcaca ttgagtctgt acagattctg gaacagcagc cgcaccagtt tcagcaacca
      781 gtcggtgacg aacaggctga cctgtgcgat ctcgttgtcg aactgttcct gccagcgctc
      841 cagccgttcc gtccagacat cctggccgta gccgcactgc cacagccagt ggctgacggt
      901 gttgatccag atataggcca tcatggaaga gcgttgcgaa aagtggccga attcgtgcgc
      961 cagtacgccc atgaactgct gcacgctggt gcccatcacc agggacatgc caatgaccag
     1021 tttgagttcg cctttcgcca gactggccat gccgtgcacc gggccggccg cggcattcat
     1081 gtcgggaatc acttcaatga actgcggcgc cggcacaccg atggcggccg tcattttttc
     1141 aatcagcctg taaaagcgtg cgtgtttttt gcgatccagc acgtacggtt tgggcggctt
     1201 gccctggggc cacagcgggt acagcagaaa caaaatcagc accgatcctg ccaccgccgg
     1261 taccacccag gacaacgccc ccaggaacag cagaaatttg ccgtgccact cgattccgcc
     1321 aaacagcaag ccatggccgc tgctgaaata ccagaccagc gcccacaccg acagcaggat
     1381 cagccccacg tagatcagcg gcgacaccag cgaggtcgcc gccgcgatgg caaaggattt
     1441 ctggtagtcg cgggtcagcg tggtgggcgc gatgggctgg gcgaaggtgg attccagtaa
     1501 tgtgaacacc tcgtcggcct ggctgcgcgc ggcgttcacc ggcggcggcg ccgctacttc
     1561 ataggcctcg attttcacca gcagcccggc ctcggtgaat ttttccagat acgccagcgc
     1621 cttcggatta tccagatcct ttttgatgac gagcggcttc tgcagcaaca tggctgcctg
     1681 ctctgccttc atgccgatac gggccagccc ctggatcacg tcctgcggca ttttgccctt
     1741 gacgggcgcg gcctgggaaa cgacgcggta ttgtttaggc atggttcagg ctgactccaa
     1801 tcaggccgat tcgccgactt tgcgtgccat ctcgtcggtg gcacgcacca gggcatcgat
     1861 gatgcccgct tccgccgccg agtggccggc atcgcggatg atctgcagct cggaattgac
     1921 ccattcatga tggagatcag tggcattgtc cagcgggcag atcatgtcgt accggccgtg
     1981 cacgatcatg cccggaacgt gcttgatgcg atccatgtta cgcagaatct gattttcctc
     2041 caggaaggaa tggtgcttga aataatgcac ttcgatcagc gccaggctca ccgccgtgtg
     2101 ggtatcagtg aaatggtcca gcacattgtt gttggggcgc agcgtcgcgc agtgtccttc
     2161 ccacgcagac caggctttcg ccgccgacat gcgctgcaga tcgttgttgc tgtgcagcaa
     2221 cttgtcgtag gcagcgatca gatcgttgcg catgttttcc cgcaccggct tgatcagatc
     2281 ggtccaggca tcgggaaaaa tgcgattggc tccatccttg taaaaccagt ccagatcttt
     2341 ttgccgggac aggaaaattc cgcgcacgat catgccgcgc acgcgctccg gatgcgtctg
     2401 cgcatacacc agcgccagag tggagcccca ggaaccgccg aacagaaccc agtcgtcgac
     2461 ctgcaactgc gtgcggatgg tttcgatatc ggccaccaga tgctgggtgg tattcagttc
     2521 cagacagcca tgcggtgtgg agcggccaca accacgctga tcaaacagca cgatgcggta
     2581 tttctgcgga tcgaaaaagc ggcggtgata ggattcgcaa ccggcaccgg ggccgccatg
     2641 caggaacagc acgggaatgc catccgggtt gccgcattcc tccacgtgca acacgtgcgg
     2701 cgcgtccaca ttaatcttgt gttctctgta gggttttatc tcaggataca gaacctgcat
     2761 cgagcggtac tcctcacggt caatccgtta actgtagttc attgataccg ctagagaaag
     2821 ccgatacgcc cccgaattcc ggcgcagccg ggccccgccg cgaaaaaggt ctacaatttc
     2881 cgagtagtgt ccaatacctt gaaattcaag tactcacctg atgattggaa ccacggtttt
     2941 cccgattctg cgcagcggtg gcatcgcgct g
//
LOCUS       DASUEG010000236         7282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1507137, whole genome shotgun sequence.
ACCESSION   DASUEG010000236 DASUEG010000000
VERSION     DASUEG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507137"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2560
                     /locus_tag="VFX11_04440"
     CDS             <1..2560
                     /locus_tag="VFX11_04440"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4937900.1"
                     /translation="EIRMFSPILNMLKALQLMSGSSSPRLWCVTQSAVAAQRSDTDLN
                     PAQTALLGFGKVLDMEHPEYSSVLIDVDAESDERAARAIITELQLPAKEKQVAYRRGS
                     RMVARLARTGVAAQGMPIPPAPYRLIIEKKGTFEDLKFVQFTPRVLGSTQVAVKVLSA
                     GLNFRDVMGVLNVYPGEAGPLGGECIGEIIELGSEVKKYSVGDVVMIPLTESCMSTQT
                     IAEEALICRVPRNLSLNEASTLPVVYCTALHGLKNLAKLKKGERVLIHAGAGGVGLAA
                     IYIAKHIGAEVFATASEKKRDYLRKIGVEHVFDSRSLDFAQEIRRVTGGEGVDVVLNS
                     LAGEFITTSMALLNPGGRFIEIGKADIWTQDRVQAFRTDITYEAFDLVIVTLQNPLQL
                     KELMDEIVDNVEAGHYKALPYTVFKHKEAMEAFRYMAQGKHIGKILINRDDPAITIKR
                     DRSYLITGASGGLGMLFAKWFAEQGAGEIILAARRDVRELDAAGVAAIEALGAKITTV
                     KADTADRASVHGMIESVKGSSLPLAGVIHAAGVLADAFVVNQDIAGFKKVMGPKLAGA
                     WYLHEETKHIDLDMFVLFSSLSSMLGAPGQANYAASNAFLDGLAQYRRAKGLPAIAVN
                     WGPWAEVGMAANSQVEANAKASGVHLIPPADGLAWFGQVLESNPIQRGLMVIDWAALA
                     NMSGSIPAFLSELDVKASVGLDANLQKMADEFRANLADAPIDERTPMLIDMICEQIKR
                     VMGLDESDTINPNQPLQELGLDSLMAVELRNILCALIGKQLPATLMFKYPTVSSLSTF
                     LIQDMFPDEVAAAEPVVAEQTQEEKATEEDDQLDDLSQDELAAMLAAELGNDKD"
     gene            2636..7267
                     /locus_tag="VFX11_04445"
     CDS             2636..7267
                     /locus_tag="VFX11_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX4937901.1"
                     /translation="MSGKSTQDLMLNALKEIKTLKRQLAAYEKAAHEPIAIIGMGCRY
                     PGQSVSPAEFWNKLYNGVDCITQQENNERWNMDDLYDPNPDAPGKIYSKGLGIIERPD
                     MFDAEFFGVSPREAEDIDPQHRILLEVCHEAMEDAGYAPSRLNGSKTGVFVGIASSDY
                     AHLGSMLGSADKLTPWQGIGNAGSAAPCRISYLYNLKGPALGIDTACSSSLVALHYAC
                     QSLRNRECDAAFSGGVHLVLNPAISIVFAKAHMLSADGKCKTFDAAADGYVRSEGCGV
                     VMLKRLSDAVADGDNILALIKGTAINQDGKSQGFTAPNETAQEEVIRAALQQAQLEPA
                     QVSYVEAHGTGTPLGDPIELGALNSVYCAGQNRRHPLLVGSAKTNIGHSEAAAGAAGL
                     VKLVLSLQHRTIPPHIHYKTPNPYIPWSKMAIQVTDKLTQWDNFAAPDGKLRAGLSSF
                     AFTGTNAHIILEEYKPEPVTESPTATAPWVPSLVLTSAKQPLAVSLRQDALKNHMDVN
                     TSLSVAQIARTCAAGRDHYKFRTALLPQSAEHLKALLVAKKGEGVLSGVKQKPKYKIA
                     FVFSGQGAQFAGMGQAFYDHIPAFRTHFDQVAAQLNPLLDVELTQLMWGEHSDKLDQT
                     RYTQPALFALEYALAKTWMQWGVKPDCVSGHSVGEITAACIAGVFSLADACKLIVARA
                     HLMGTTAQGAMLVVFANEAKTRTLIQASPGLEICAVNGPLNTVVGGDPALVDALEAQL
                     AGQDIQFRRLPVQHAFHTAAMDSALKPFAEKLNGIAFTSPRMRIYSNLNGEHVGKQMA
                     EPDYWVQQIRQPVQFMACAKNIIDAQCDLVLEVGPGTALIAMLQEVASSLRAGGQDLQ
                     PLVTVGSLQKKIPALDALTRSLAQLYVAGLDLDWAEIMPLKQVAPVELPTYPFQRKSF
                     WNETLREALSQQEMPEKARNWLYQMRWDAAPAPQVAAGAIPSKRWVLLFPTLSLAEQF
                     SALLPAAQARQCGSIAIDNGQVTLNLNGSREMLPVDGSVSPWLASVLQGSAAEPVQLV
                     ISLAQLPLVVTAADYDALTALFLGLAKTLQTVSHCHLSIITEAAQGTKGQQAVVRADQ
                     AVVLGFAKNLLLELADRMQSTIDVEDWTAATAQLCANALQAVQPSPLLSVRQNQLLTP
                     SLLRVEGERLSQRKLNLNANSFYVVAGGTGAIGLAVANWLAGKGALHIALLSRNGARG
                     EDAQQQIETLRKNGVQVVVPAVDIADRAALDRVLQSLRAEHPIAGVIHAAGQFELVPV
                     GELTTARCSQLMANKVLGLQWLDELCADDKLEFFAGFSSIASVWGSAGNFHYSAANQA
                     VDAIIQRRRQQGKPALAINWGPWAESGMVSDESGAQAAKRGLLQLEKNIGVQTFGRLL
                     GSDEIQITIADIQWNRFKPLMSMLPVGALFNRVNDLNGDAPAEVVVELNQEDIAFKEE
                     LATCAPEERADRLLVYLRHQLARALQLNALEVDDTQPLIDMGIDSLLAVEFKNRVTQV
                     TQVELPVVRLLGGASLRDVAKWMAEGYGDLNQADQPVLDDEQALVEGVL"
BASE COUNT         1496 a         2111 c         2309 g         1366 t
ORIGIN      
        1 ggaaatccgc atgttctcgc ccatcctgaa catgctgaag gcactccagc tgatgtccgg
       61 ttcgtcgtcg ccgcgcctgt ggtgcgtgac ccagtccgcc gttgccgcac agcgcagcga
      121 caccgacctg aacccggcgc agaccgcgct gctgggcttc ggcaaggtgc tggacatgga
      181 gcatccggaa tattcgtccg tgctgatcga tgtggatgcg gaaagcgacg agcgtgccgc
      241 ccgtgccatc atcaccgaac tgcaattgcc ggcgaaggaa aagcaggttg cctatcgtcg
      301 tggcagccgc atggtggccc gtctggcgcg caccggcgtt gccgcgcagg gcatgccgat
      361 tccgccggcg ccgtaccgtc tgatcatcga gaagaaaggt acgttcgaag atctgaagtt
      421 cgtacagttc acgccgcgcg tactgggttc cacccaggtg gccgtgaaag tgctgagcgc
      481 cggtctgaac ttccgcgacg tcatgggtgt actcaacgtg tacccgggtg aagccggtcc
      541 gctgggcggc gaatgtatcg gtgaaatcat cgagctgggc agcgaagtca agaaatacag
      601 cgtgggcgat gtagtgatga tcccgctgac cgagtcgtgc atgagtaccc agaccatcgc
      661 tgaagaagcc ttgatctgcc gcgtgccgcg caacctgtcg ctgaatgaag cctcaaccct
      721 gccggtggtg tactgcaccg cgctgcatgg tctgaagaac ctggccaaac tgaagaaggg
      781 cgagcgcgtg ctgattcatg cgggcgccgg tggtgtcggt ctggcggcga tttacatcgc
      841 caagcatatc ggcgcggaag tgtttgccac cgccagtgag aagaagcgcg attacctgcg
      901 caagatcggt gtcgagcacg tgttcgattc ccgttcgctg gatttcgccc aggagatccg
      961 ccgcgtaacc ggtggtgaag gtgtggacgt ggtgctgaac tcgctggccg gtgaattcat
     1021 caccaccagc atggcgctgc tgaatccggg cggccgcttt atcgagatcg gcaaggcgga
     1081 catctggacc caggatcgcg tgcaggcgtt ccgtaccgac atcacctacg aagcgttcga
     1141 cctcgttatc gtcacactgc agaacccgct gcagctgaag gaactgatgg acgagatcgt
     1201 cgacaacgtg gaagccggtc attacaaggc gctgccctac accgtgttca agcacaagga
     1261 agcgatggaa gccttccgct acatggcgca gggcaaacac atcggcaaga tcctgatcaa
     1321 ccgcgacgat ccggcgatca ccatcaagcg cgatcgctcg tacctgatca ccggtgccag
     1381 cggcggtctg ggcatgctgt tcgcgaagtg gtttgctgaa cagggcgcag gcgaaatcat
     1441 cctggccgca cgccgcgacg tgcgtgaact ggatgccgcc ggtgtcgctg cgatcgaggc
     1501 gctgggagcg aaaatcacca ccgtgaaagc cgacactgcg gatcgcgcat cggtgcatgg
     1561 catgatcgaa agtgtgaaag gtagcagcct gcccctggct ggtgtcattc acgccgccgg
     1621 tgtgctggcc gacgccttcg tggtgaacca ggacatcgcc ggattcaaga aggtcatggg
     1681 tccgaagctg gcaggtgcct ggtatctgca tgaagaaacc aagcacatcg atctggacat
     1741 gttcgtgctg ttctcgtcgc tgtcgtccat gctgggtgcg ccgggtcagg ccaactacgc
     1801 ggcctcgaac gccttcctgg atggtctggc gcagtaccgc cgcgcgaagg gcctgccggc
     1861 catcgccgtg aactggggcc cttgggccga ggtgggcatg gcggccaaca gccaggtgga
     1921 agccaatgcg aaagccagcg gcgtgcacct gattccgccg gccgatggtc tggcctggtt
     1981 cggccaggtg ctggagtcca acccgatcca gcgcggcctg atggtgatcg actgggctgc
     2041 tctggccaac atgtccggca gcatccccgc gttcctgtcg gaactggatg tgaaagccag
     2101 cgtgggtctg gatgcgaatc tgcagaagat ggcagacgaa ttccgcgcca atctggcgga
     2161 cgcacccatc gacgagcgca cgccgatgct gatcgacatg atctgcgaac agatcaagcg
     2221 cgtcatgggt ctggacgagt cggataccat caaccccaac cagccgttgc aggaactggg
     2281 tctggattcg cttatggcgg tggaactgcg taacatcctg tgcgcgctga tcggcaagca
     2341 gctgccggcg acactgatgt tcaagtaccc caccgtgtcg agtctgagta ccttcctgat
     2401 ccaggacatg ttcccggatg aggtggccgc cgctgaaccg gttgtggcag agcaaacaca
     2461 agaagaaaaa gcgacggaag aagacgatca gctggatgat ctgtcccagg acgagctggc
     2521 tgccatgctg gctgccgaac tgggcaacga caaggactga tcgcaataaa gtccgtcaac
     2581 agctgacacc aggcgccttc actggcgcct ggttttaaaa aataggggca gcaacatgag
     2641 tggcaagtca acgcaggact tgatgctgaa tgcgttaaag gaaatcaaaa cgctgaagcg
     2701 tcagctggcg gcctatgaga aagcggccca tgaacccatc gccatcattg gcatgggttg
     2761 ccgttatccg gggcagtcgg taagcccggc agaattctgg aacaagcttt acaacggagt
     2821 ggattgcatt acccagcagg aaaacaacga acgctggaac atggatgatc tgtacgaccc
     2881 gaaccccgat gcgcccggca agatctattc gaaaggcctg ggcatcatcg agcggccgga
     2941 tatgttcgac gctgaattct tcggcgtgtc gccccgggaa gcggaggata tcgatccgca
     3001 gcaccgcata ctgctcgaag tctgtcacga agcgatggaa gacgccggct atgcgccgtc
     3061 ccgcctgaat ggcagcaaga ccggcgtgtt cgtcggtatc gcttcctccg attatgcgca
     3121 cctgggcagc atgctgggga gcgccgataa actcacaccc tggcagggca tcggcaacgc
     3181 cggcagcgcc gcgccgtgcc ggatttccta tctgtataac ctgaaaggcc ctgcgctggg
     3241 catcgacact gcctgttcgt cgtcgctggt ggcactgcat tacgcctgcc agagcctccg
     3301 caaccgtgaa tgcgatgcgg ccttttccgg tggcgtgcat ctggtgttga atccggcgat
     3361 ctccatcgtg tttgcgaaag cacacatgct gtccgccgat ggcaaatgca aaacgtttga
     3421 cgccgctgcc gatggctatg tgcgctccga aggttgtggc gtggtgatgc tgaaacggct
     3481 gtcggacgcg gttgccgacg gtgacaacat tctcgcgctg atcaagggca ccgcgatcaa
     3541 ccaggacggc aagtcgcaag gctttaccgc acccaacgag accgcgcagg aagaagtgat
     3601 ccgcgccgcg ctgcaacagg cgcaactgga accggcgcag gtgagctatg tagaagcgca
     3661 cggcactggc accccgctgg gcgatcccat tgagctgggc gcactgaatt ccgtgtattg
     3721 cgcgggacag aatcgccggc atcctctgct ggtgggttcc gccaaaacca atatcggtca
     3781 cagtgaagcg gccgccggtg ccgccggact ggtcaagctg gtgctgtcat tgcaacaccg
     3841 cacgattccg ccgcacattc attacaaaac gccgaatccc tatattccct ggagcaagat
     3901 ggcgatccag gtgaccgaca aactcaccca gtgggacaac tttgccgcac ccgatggcaa
     3961 gctgcgcgcc ggcctgagtt catttgcgtt caccggcacc aacgcccaca tcattctcga
     4021 ggaatacaag ccggaaccgg taaccgaatc accgacagcg accgcaccct gggtgccgtc
     4081 actggtgtta acctctgcca aacagcccct ggcagtgtcc ctgcggcagg atgctctgaa
     4141 gaatcacatg gatgtgaata cttctctcag cgtggcgcag atcgcgcgca cctgcgccgc
     4201 cggacgggat cactacaaat tccgcacggc cctgctgccg caatcggcgg aacacctgaa
     4261 ggcattgctt gttgccaaaa aaggcgaggg cgtgctgtcc ggtgtgaagc agaagccgaa
     4321 atacaagatc gcgtttgtgt tctcggggca gggcgcccag tttgccggca tggggcaggc
     4381 attctacgat cacattcccg cattccgcac ccatttcgac caggtggcgg cgcaactgaa
     4441 tccgctgctg gatgtggaac ttacccagtt aatgtggggt gagcacagcg ataaactgga
     4501 ccagacccgt tatacccagc cggccttgtt cgcgctggaa tatgcgctgg caaaaacctg
     4561 gatgcagtgg ggcgtgaaac ccgattgcgt gtcgggtcac agcgtcggtg aaatcaccgc
     4621 tgcctgtatt gccggcgtgt tcagtctggc cgatgcctgc aagctgatcg tggcgcgggc
     4681 acacctgatg ggcaccaccg cgcagggtgc catgctggtg gtgtttgcca acgaagcgaa
     4741 aacccgcacg ctgattcagg cgtcacccgg cctggaaatc tgcgccgtga atggcccgct
     4801 caataccgtg gtgggtggcg atcccgcgct ggtggatgcg ctggaagcac aactggctgg
     4861 gcaggacatc cagttccgcc gcttgccggt gcaacacgca tttcacaccg ccgcgatgga
     4921 ttccgccctg aagccgtttg cggaaaaact caacggcatc gctttcacca gtccgcgcat
     4981 gcgcatctat tccaacctga atggcgagca tgtcggcaag cagatggccg agccggatta
     5041 ctgggtgcag cagatccgtc agccagtgca gttcatggcc tgcgcgaaaa acatcatcga
     5101 tgcgcaatgt gatctggtgc tggaagtcgg ccctggcact gcgctgattg ccatgttgca
     5161 ggaagtggcg tcgtcgttgc gtgcgggcgg tcaggacctg cagcccctgg taacggtggg
     5221 cagtctgcag aaaaaaatac ctgcgctgga tgctctgacc cggtcgcttg cgcagctgta
     5281 tgtggcggga ctggatctgg actgggcgga aatcatgccg ctgaaacagg tcgcgccggt
     5341 ggagctgccg acgtatccat tccagcgcaa gagcttctgg aatgaaacct tgcgagaagc
     5401 gctgtcgcag caggaaatgc cggaaaaggc ccgtaactgg ctgtaccaga tgcgctggga
     5461 cgcagcacct gcaccgcagg tggcggccgg agcgataccg tcgaaacgtt gggtgctttt
     5521 gttcccgacg ctgtcactgg cggaacagtt ctccgcgctg ttgccagccg ctcaggcaag
     5581 gcagtgtggc agtattgcca ttgataacgg ccaggtgacc ttgaacctga acggtagtcg
     5641 ggaaatgctg cccgtcgacg gcagtgtcag tccatggctg gcgagcgtgt tgcaaggcag
     5701 cgccgccgag ccggtgcaat tggtgatatc actggcgcag ctgccactgg ttgtaacagc
     5761 agcggattat gatgcgctca ctgcgctgtt cctggggctg gcgaaaacgt tgcagaccgt
     5821 ttcccattgc catctcagca tcatcactga agcggcgcag ggcacgaagg gccagcaggc
     5881 cgtcgtgcgg gccgatcagg ccgtcgtgct cggctttgcc aagaacctgc tgctggaact
     5941 ggcagaccgg atgcagtcca ccatcgatgt ggaagactgg actgcagcga ccgcgcagct
     6001 gtgcgcgaat gcattgcagg cggtacagcc ttcacccttg ctgagcgtgc gccagaatca
     6061 attgctgacg ccctcgttgc tgcgggtgga aggcgagcgt ttgtcgcagc gcaaactgaa
     6121 cctgaacgcc aattcttttt acgttgtggc cggtggcact ggcgccattg gtctggctgt
     6181 ggcgaactgg ctggcaggca agggcgcgct gcatatcgcg ttgctcagcc gcaacggtgc
     6241 ccgtggtgag gacgcgcagc agcagattga aaccctgcgc aagaacggtg tgcaggttgt
     6301 ggtgcccgcc gtggacatcg ccgaccgtgc cgcgcttgat cgcgtgctgc aatccctgcg
     6361 tgcagagcat cccattgcgg gcgtgattca cgccgccggc cagttcgaac tggtgccggt
     6421 gggcgaactc accactgcac gttgcagcca gctgatggcg aacaaggtgc tgggcctgca
     6481 atggctggat gaactctgcg ctgacgacaa gctggaattt ttcgccggct tttcctcgat
     6541 tgcatcggtg tggggttcgg cgggcaactt ccattattcc gccgccaacc aggccgtgga
     6601 cgccatcatc cagcgccgtc gccagcaggg caaaccggca ctggccatca actgggggcc
     6661 gtgggcggag tccggcatgg tcagtgacga gtccggcgcc caggcagcca agcgcggcct
     6721 gctgcagctg gaaaaaaata tcggcgtgca gacctttggc cgcctgctgg gcagcgacga
     6781 aatccagatc accattgccg acatccagtg gaatcgcttc aagccgctga tgagcatgct
     6841 gccggtgggg gcgctgttca atcgtgtcaa cgatctgaac ggcgatgctc cggcagaagt
     6901 agtggtggaa ctgaatcagg aagacatagc cttcaaggaa gagctggcca cctgcgcgcc
     6961 ggaagaacgg gcggatcgtc tgctggtgta tctgcgtcac cagctggccc gcgcactgca
     7021 gctcaatgca ctggaagtgg acgacaccca gccgctgatc gatatgggca ttgattccct
     7081 gctggcggtg gagttcaaaa accgtgttac gcaggtgacg caggtggaac tgccggtggt
     7141 gcgtctgctg ggcggcgcca gtctgcgcga cgtggcgaag tggatggccg aaggatacgg
     7201 cgatctgaat caggccgatc aaccggtgct ggatgatgag caggcgctgg tcgaaggcgt
     7261 attataagaa gagttccgga ag
//
LOCUS       DASUEG010000237         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_424537, whole genome shotgun sequence.
ACCESSION   DASUEG010000237 DASUEG010000000
VERSION     DASUEG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424537"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFX11_04450"
     CDS             complement(<1..562)
                     /locus_tag="VFX11_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX4937902.1"
                     /translation="MSALFVKHLTVLDFSYVHPERGILGESWIVDVELHGDLDDQGMV
                     FDFGHVKRQVRDAVESLFDHKLVVPKSLPGLQVQRQGDQLDLQWQDLAGRTFHHLSPS
                     EGVELVEAAEVTPDTLVDQLQQTALAAVPSNVKKVVIELRTEDISGAFYHYSHGLKKH
                     DGNCQRIAHGHRSRLKILRNGKRDPLL"
     gene            657..1994
                     /locus_tag="VFX11_04455"
     CDS             657..1994
                     /locus_tag="VFX11_04455"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4937903.1"
                     /translation="MSSVLITHAKVVNEGRIAELDVLVRNGRIEKIGADLSSHKADRI
                     IDATGKHLLPGMIDDQVHFREPGLTHKGEIATESAAAVAGGITSYMEMPNVNPLTINR
                     AALEDKYDRAVQKSLANFAFYLGASNDNLDEIKAIDPNAACGIKVFMGASTGNMLVDK
                     RETLEGIFANSPIIVVTHCEDSPTIKLNEDRYREQYGEDVPMACHPLIRSEEACWKSS
                     TLAVELAKKHGTQLHILHLTTAKELALLTPGDLAGKRITGEACAHHLYFNEADYATKG
                     ALIKCNPAIKTAADQEALLNAVNDGRIDVIATDHAPHTWDEKQQSYFKAPSGLPLVQH
                     ALISLLEHYHNGRFSLETIVQKTSHNVARLFQIAERGFIREGYWADLVLVDLTAPTVV
                     DQQKILYKCGWSPFAGTIFKSSIVTTLVNGDIAFENGQVNSAVRGKRLAFNRR"
     gene            2125..>2333
                     /locus_tag="VFX11_04460"
     CDS             2125..>2333
                     /locus_tag="VFX11_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX4937904.1"
                     /translation="MIGFGRKPRICIVGGGFAGLNAAQQLKSSRYDVTVIDPSPHIEW
                     LPNIHEIISGVKKGDELRLNRAILLR"
BASE COUNT          511 a          740 c          660 g          422 t
ORIGIN      
        1 ccagcagcgg gtcgcgcttg ccgttgcgca ggatcttcag gcgtgaccgg tggccgtggg
       61 caatgcgctg gcagttgccg tcatgctttt tcaggccgtg ggaatagtgg tagaaagcgc
      121 cggagatgtc ctcggtacgc agctcgatca ccactttctt cacgttggac ggcacggcgg
      181 ccagggcggt ctgctgcagc tgatccacca gggtatccgg cgtgacttcc gctgcctcca
      241 ccagttccac gccctcggat ggcgacagat gatggaatgt gcggccggcc aggtcctgcc
      301 actgcagatc cagctggtcg ccttgccgct gcacctgcag gcccggcagg ctcttcggca
      361 ccaccagctt gtgatcgaac agggattcca ccgcgtcgcg cacctgccgc ttcacatggc
      421 cgaagtcgaa caccatgccc tggtcgtcca gatcgccgtg caattccacg tcgacaatcc
      481 agctttcgcc cagaatgccg cgctccggat gcacgtagga aaaatcaaga acggtcaggt
      541 gtttaacaaa cagtgcagac atgggacaag gctcggggtc cagtacggga gggcaatcag
      601 gtataatacg ctgcccctgt tgccggtgcc aattcagtca ttataggaag cgaatcatgt
      661 ccagtgtgtt aatcacccat gcgaaggttg tcaacgaagg ccggattgcc gaactggatg
      721 tgctggtgcg caatggccgc atcgagaaaa tcggcgccga tctgtccagc cacaaggccg
      781 atcggatcat cgacgccacc gggaaacacc tgctgcccgg catgatcgac gaccaggtgc
      841 atttccgcga accgggcctg acccacaagg gcgagatcgc caccgaatcc gccgcagcgg
      901 tggcgggcgg catcaccagc tacatggaaa tgcccaacgt gaatccgctc accatcaatc
      961 gcgccgcgct ggaagacaag tatgaccgcg ccgtgcagaa atccctcgcc aattttgcgt
     1021 tctacctggg tgccagcaac gacaacctgg acgaaatcaa agccatcgac cccaacgccg
     1081 cctgcggcat caaggtgttc atgggcgcct ccaccggcaa catgctggtg gacaagcgcg
     1141 agaccctgga aggcattttc gccaacagcc ccatcatcgt cgtcacccac tgcgaggatt
     1201 cgccgacgat caagctgaac gaggaccgtt accgcgaaca gtacggcgag gacgtgccca
     1261 tggcctgcca tccgctgatc cgttccgagg aagcctgctg gaaatcctcc accctggcgg
     1321 tggagctggc aaaaaagcac ggcacccagc tgcacatcct gcacctgacc acggccaaag
     1381 aactggccct gctcacgccc ggcgatctgg cgggcaagcg catcaccggc gaagcctgcg
     1441 cgcaccatct gtatttcaac gaagccgatt acgccaccaa gggcgcactg atcaagtgca
     1501 acccggccat caaaaccgcc gccgatcagg aagccctgct caatgccgtc aacgacggcc
     1561 gcatcgacgt gatcgccacc gatcacgcgc cccacacctg ggatgaaaaa cagcagtcct
     1621 atttcaaggc accatcgggt ctgccactgg tgcagcacgc gctgatttcc ctactggagc
     1681 attaccacaa cggccgcttc agcctggaaa ccatcgtgca gaaaaccagt cataacgtgg
     1741 cgcgcctgtt tcagatcgcc gagcgcggct tcatccgcga aggttattgg gccgatctgg
     1801 tgctggtgga tctgaccgca cccacggtgg tggaccagca gaagattctc tacaagtgcg
     1861 gctggtcgcc gttcgccggc accatcttca agtcgtccat cgtcaccacc ctggtgaatg
     1921 gcgacatcgc gttcgagaac ggccaggtca acagtgcagt acgtggcaag cgtcttgctt
     1981 tcaaccgcag gtaatcggtt gccggcaaag ggcggccgtt acaaaattgt cgcctgatta
     2041 aaggttcagg ctttgcgttc tgggatagaa tgcgcgcact ttgtaaacag cgtgtgcacc
     2101 aatcaggatt gagggagcaa cgacatgatc ggatttggcc gcaaaccccg catttgcatc
     2161 gtcggcgggg gatttgccgg gctcaatgcc gcccagcagc tgaagtcgtc ccgttacgac
     2221 gtcaccgtca tcgatccgtc gcctcatatc gaatggctgc ccaacattca tgaaatcatc
     2281 tccggcgtaa aaaagggcga cgagctgcgg ctcaaccgcg ccatcctgct acg
//
LOCUS       DASUEG010000238         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1937386, whole genome shotgun sequence.
ACCESSION   DASUEG010000238 DASUEG010000000
VERSION     DASUEG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937386"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1301)
                     /locus_tag="VFX11_04465"
     CDS             complement(66..1301)
                     /locus_tag="VFX11_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX4937905.1"
                     /translation="MSKPRIYILGGYQSDFAQNWARQGVTLFDSFANAVRKGLESAAL
                     DAHELGVGHVGNFVSDLFTGQAHLGGFFGHVDPALCYLPASRHEAACASGSMALLAAM
                     ADLEAGRYDLACVLGIEMMRNVSGDQAAANLRAAAWVEREWCDTPYVWPCAFDQLVEV
                     YRQRHGIDRVHLAAISEKNFANARRNPNAQTRHWQFGVDSFTANDAANPVISGHIRRQ
                     DCGQITDGAAVVFLATESRARQYAQKRGIALDELPWIQGWGHINAPMLLAEKLLHAEP
                     GGYLFPHIRELFRQTLARAGLPDVRAVDGLEVHDCFNITEYMILDHTGLNAPGEAWRA
                     IENGTTARGGRLPVNASGGLIGLGHPVGATGVRMALDCYRQVRGIAGDCQIEGARRMM
                     TFNLGGSTTTCASLIIGRS"
     gene            complement(1298..2785)
                     /locus_tag="VFX11_04470"
     CDS             complement(1298..2785)
                     /locus_tag="VFX11_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX4937906.1"
                     /translation="MPILYQNNVQHTLQPSSHPYLNGAWTPNFTEYTATDLEVIGTIP
                     ADIDGIYLRNTENPVQEPIGLYHPFDGDGMLHAMLFRDGKAEYRNRFIRTKGFNAEQE
                     AGAAIWAGIANGIDKSKRPGWGAQGFVKDASSTDVVVHAGNVMTTFWQCGDGYRLDPF
                     TLEQLGAEGWTPLDGISAHPKVDARTGEMLFFNYSKYPPYQHYGVVNRDNRLVHYTPV
                     PLPGPRLPHDMAFTENYSILVDLPLYWDPKLLAQGIHHAEYFPNQPTRFGILPRYGTA
                     ADVKWFEAAPTYVLHWNNAYEDGDEIVLDGYFQEDPNPPPLPGMPARAGKMMANLDMH
                     SFKPHLHRWRFNLRTGEVKEQVLDARVLEFGTFNQRYAGRKSRYAYSVTSKPGWFLFT
                     ALIKHDLETGESWRLDFGDERYGSESPFAPRVNAQDEDDGYLVSFITDMKLDRSECVL
                     IDAKNIEAGPVCRIILPERICSGTHATWADGPALRAQRKTEGNVP"
     gene            2952..3896
                     /locus_tag="VFX11_04475"
     CDS             2952..3896
                     /locus_tag="VFX11_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4937907.1"
                     /translation="MTQSQNGQDMMEQALILNRDMVTALDIIGDRWTLLILRDTFLGR
                     CRFEEFRQHTGASRATLSQRLERLVAGDILYRRAYGEGSNRFEYRLTVKGLELFGASL
                     LAWQWEREWSPQDDLPRQLHHALCGHPLQPRAQCRNCQATLKIRDVSWPAGILGAQFS
                     EIRSLSGQRRARSDARGSDSALTTISDLIGDRWTLLILIAAFFGVKRYDEFQKQLGIA
                     SNILTARLSRLVDAGIVNRRQYQQNPPRSDYRLTTKGESLYPLVIVLRQWAGDRRSES
                     GSTATLLHSCGQPLTVDVVCGNCGQKPWPKDVVTFARS"
     gene            complement(3927..4274)
                     /locus_tag="VFX11_04480"
     CDS             complement(3927..4274)
                     /locus_tag="VFX11_04480"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4937908.1"
                     /translation="METKLSEQVCEACRADAPSVSDAELAQFMRQLPGWAPVVRDGIL
                     QLERQFSFPDFAAALAFTNRVGALAEAAGHHPALLTEWGKVTVTWWTHKIKGLHKTDF
                     ILAARTDEVVNGA"
     gene            4545..>5718
                     /locus_tag="VFX11_04485"
     CDS             4545..>5718
                     /locus_tag="VFX11_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937909.1"
                     /translation="MPQYKAPLRDMNFVMNEVLDYPGHYAKLPCAENSTPDMVEAILS
                     ECAKFCEEVLSPLNQSGDIEGCHWENGDVTTPKGFKEAYAQFCQGGWQGLSHPEEFGG
                     QGLPLSLSIMKSEMIGTANWSWGMYPGLSLGAMNTIFIHGTVEQKHHFLPKMVEGTWS
                     GTMCLTEPQCDTDLGQVKTKAEPLPNGAYAISGTKIFISAGEHDLAENIIHIVLARLP
                     DAPPGTKGISLFIVPKIKCDPAGNLGARNTVTCGSIEHKMGIRASATCVMNFDKAEGY
                     LIGPPNKGLECMFTFMNSARVGTSLQGLGAAELSYQGALPYAKERLSMRSLSGKKFPD
                     KVADPLIVHGDVRRMLLTQKAFAEGGRAMIYYAAKFTDHLIFGKTQEEKDEADDQLG"
BASE COUNT         1190 a         1852 c         1589 g         1087 t
ORIGIN      
        1 gtcaggcatg ccgacacaac cactgaggca ggccagcaac agcagaagca tccccgattt
       61 tttcatcatg atctccctat gatcaaactg gcgcaggtgg tggtactgcc ccccaggttg
      121 aacgtcatca tacgccgggc gccttcaatc tgacagtcgc cagcgatgcc acgcacctgc
      181 cgatagcaat ccagtgccat gcgcacgccc gtggcaccga ccggatgtcc cagcccgatc
      241 agaccgccac ttgcgttcac cggcaatcgc ccgccacgcg ccgtcgtgcc gttctcgatg
      301 gcgcgccagg cttcacccgg cgcattcagg ccggtgtgat ccagaatcat gtattcggta
      361 atgttgaagc agtcgtgcac ttccagcccg tccactgccc gcacgtcggg caaccccgcc
      421 cgcgccagcg tctgccggaa cagttcgcgg atatggggaa acagataacc gccaggttcg
      481 gcatgcaaca gcttttccgc cagcagcatc ggcgcattga tgtgacccca gccctgtatc
      541 cacggcaact catccagcgc aattccgcgc ttttgcgcgt actggcgcgc gcgggattcc
      601 gtggcaagaa acaccaccgc ggcaccatcg gtaatctgcc cgcaatcctg gcggcggatg
      661 tgtccactga tcaccggatt ggctgcgtcg ttcgccgtaa aactgtcgac gccgaactgc
      721 cagtgacggg tttgcgcatt ggggttgcgc cgcgcattgg cgaaattctt ttccgagatc
      781 gccgccagat ggacgcggtc gatgccatgc cgctgccggt atacctccac cagctgatcg
      841 aatgcgcacg gccacacgta cggcgtgtcg caccactcgc gctctaccca cgcagccgca
      901 cgcagattgg cagccgcctg atctccgctc acattgcgca tcatctctat acccagcacg
      961 cacgccaggt cgtaacggcc cgcttccaga tccgccatcg ccgccagcag cgccatcgaa
     1021 cccgaggcgc acgccgcttc atgtcgcgat gccggcagat agcagagcgc cggatcaacg
     1081 tgaccaaaaa atccgcccag atgcgcctgc ccggtaaaca gatcgctgac aaaattgcca
     1141 acgtggccca cacccagttc atgcgcatcc agcgcggcgc tttccagacc ttttcgtacg
     1201 gcattggcaa aactgtcaaa cagtgtcact ccctggcgcg cccagttttg cgcaaaatcg
     1261 ctctgataac cgccaaggat gtaaatacgc ggtttgctca cggcacattt ccctctgtct
     1321 tccgctgtgc ccgcaacgcc ggcccgtccg cccaggtggc atgggtaccg ctgcagatcc
     1381 gctcgggcag aatgatgcgg cacaccggcc cggcctcaat atttttcgcg tcgatcaaca
     1441 cacattcgga ccggtccagt ttcatgtcgg taatgaaact caccagataa ccatcgtcct
     1501 catcctgcgc attcacccgc ggtgcaaatg gcgactcgct gccataacgt tcatcgccga
     1561 aatccagccg ccaggattca ccggtctcca gatcgtgttt gatcagcgcc gtgaacagga
     1621 accagcccgg cttcgacgtc accgaatagg cgtagcgcga cttgcgcccg gcataacgct
     1681 gattaaaggt gccgaactcc agcacgcgcg catccagcac ctgttccttc acttcaccgg
     1741 tgcgcagatt gaaacgccag cggtgcagat gcggcttgaa ggaatgcatg tcgagattcg
     1801 ccatcatctt gccagcgcgc gccggcatgc ccggcagcgg cggcggattc ggatcttcct
     1861 ggaagtaacc gtccagcacg atttcatcgc cgtcttcata ggcgttgttc cagtgcagca
     1921 cataggtcgg cgctgcttca aaccacttca cgtcagcggc cgtgccgtag cggggcagaa
     1981 tgccgaagcg cgtcggctga ttgggaaaat actcggcgtg atggatgccc tgcgccagca
     2041 gtttcgggtc ccagtacagc ggcagatcaa cgaggatgga ataattttcg gtgaacgcca
     2101 tgtcgtgcgg cagacgcgga cccggcagcg gcaccggcgt gtaatgcacc agcctgttgt
     2161 cgcggttcac cacgccgtag tgctggtacg gcggatattt ggagtaattg aaaaacagca
     2221 tctcgcccgt gcgcgcatcc actttgggat gcgcggaaat gccgtccaac ggcgtccagc
     2281 cttccgcgcc cagctgttcc agcgtgaacg gatcgagacg gtaaccatca ccgcactgcc
     2341 agaacgtggt catcacgtta ccggcatgca ccaccacatc ggtggacgac gcatccttca
     2401 caaaaccctg tgcgccccag cccggtcgct tggacttgtc gatgccgttg gcgatgccgg
     2461 cccagatcgc cgcgccggct tcctgctcgg cattgaaacc cttggtgcgg atgaagcggt
     2521 tgcggtattc cgccttgccg tcgcgaaaca gcatcgcgtg cagcatgccg tcgccatcga
     2581 acgggtgata aaggccaatg ggttcctgca ccgggttttc cgtgttgcgc aggtaaatgc
     2641 catcaatatc cgctggaatc gtgccaatca cctccaggtc ggtggccgtg tattcggtga
     2701 aattcggtgt ccaggccccg ttcagatagg gatgggaact gggctgcaac gtgtgctgga
     2761 cgttgttctg gtacaggatg ggcatgaccg acgctcccgg aggcagtgca gactgacact
     2821 gcaaaggttc aatgaacaaa tagtaacttg cattaagata ccaatattgc ccggcccggt
     2881 gtcaatggcg gatatttatt gcctttagac taaaatgagc aaaatttgct ggcttttata
     2941 agtgaaccaa aatgacgcag tcacagaacg gacaggacat gatggagcag gcgctgatcc
     3001 tcaatcgcga catggtgacg gcactggaca tcatcggcga ccgctggacc ctgctgatcc
     3061 tgcgcgacac ctttctgggc cgctgccgct tcgaggaatt ccgccagcac accggcgcca
     3121 gccgcgccac cctgtcacaa cggctggagc ggctggtggc gggcgacatt ctctatcgcc
     3181 gcgcttacgg tgagggcagc aaccgctttg aataccggct cacggtgaaa gggctggaac
     3241 tgtttggcgc ctcactgctg gcctggcaat gggaacggga gtggtcgccg caggacgatc
     3301 tgccccggca gttgcaccac gcactgtgcg gccacccgtt gcaaccacgg gcgcaatgcc
     3361 gcaactgcca ggccacactg aagatcaggg acgtcagctg gcccgccggc atactcggcg
     3421 cccagttcag cgaaatccgc tccctcagcg gccagcggcg cgcccgcagc gacgcccgcg
     3481 gcagcgacag cgcccttacc accatcagcg atctgatcgg cgaccgctgg accctgctga
     3541 tcctgatcgc cgcgtttttc ggcgtgaagc gttatgacga attccagaag cagctgggga
     3601 ttgcgtccaa cattctcacc gctcgcctgt ctcggctggt ggacgccggc atcgtcaacc
     3661 gcaggcaata ccagcagaat ccaccgcgca gcgattaccg cctgacgacc aagggtgaat
     3721 cgctgtatcc gctggtgatc gtgctgcgtc aatgggcggg ggaccgacgc tccgaatccg
     3781 gcagcacggc aacgctcctg cacagctgcg ggcagccact gaccgtggat gtggtgtgcg
     3841 gtaactgcgg gcagaaaccc tggccaaagg atgtcgtgac attcgcgagg tcgtgagcaa
     3901 ggccgcgaca atgaaccgga tgcgcactac gcgccgttca ccacctcatc tgtccttgcc
     3961 gccagaatga aatccgtttt gtgcagccct ttgatcttgt gggtccacca ggttaccgtc
     4021 actttgcccc attccgtcag cagcgccgga tgatgccctg ccgcctccgc cagcgcgccc
     4081 acccggttgg tgaacgccag tgcagcggca aaatcgggaa agctaaattg ccgttccagc
     4141 tgcagaattc cgtcgcgcac cacaggcgcc cagcccggca gttgccgcat gaactgcgcc
     4201 agttccgcat cactcacgct gggcgcatcc gcccgacagg cttcgcacac ctgttcactc
     4261 agcttcgttt ccatcctgca ctctcccttt gctgtcataa ccaaatggcc gatccgctgc
     4321 cggattcaga ttatttgttc attccggcct gcatcaggcc gaattttcca ggtactgccg
     4381 ctatccttgc ttaaaacaag gatttccaaa cagctgattc aaacaactgt tggctgcgct
     4441 gacggtcaaa atgatctccc ttgccaatgg ccggacgcgg tcgctcgtgc acactcgcac
     4501 acctataaaa gcccctcaac cggaatcgag aaggaagcag tgccatgccc cagtataaag
     4561 cgcccctgcg tgacatgaac ttcgtcatga atgaagtgct ggattacccc ggccattacg
     4621 ccaagctgcc ctgcgccgag aattccacgc cggacatggt ggaagcgatc ctgtccgagt
     4681 gcgcgaaatt ctgcgaggaa gtattgtctc ccctcaacca gagcggtgac attgaaggct
     4741 gccattggga aaacggtgac gtcaccacac caaaaggctt caaggaagcc tacgcccagt
     4801 tctgtcaggg cggctggcag ggtttgtcac acccggaaga attcggtggc cagggcctgc
     4861 ccctgtcgct gtccatcatg aagtcggaaa tgatcggcac cgccaactgg tcctggggca
     4921 tgtaccccgg cctcagcctc ggcgccatga acaccatttt catccacggc acggttgaac
     4981 agaagcacca cttcctgccg aaaatggtgg agggcacctg gtccggcacc atgtgtctga
     5041 ccgaacccca gtgcgacacc gacctgggtc aggtcaagac caaggccgag cccctgccca
     5101 atggcgccta cgcgatttcc ggcaccaaga ttttcatttc agcgggcgaa cacgatctgg
     5161 cggaaaacat cattcacatc gtgctggcgc gtctgccgga tgcacctccc ggaaccaagg
     5221 gcatctcgct gttcatcgtg cccaagatca agtgcgatcc cgccggcaac ctgggcgcgc
     5281 gcaacaccgt tacctgcggt tccattgaac acaaaatggg tatccgcgcc tctgccacct
     5341 gcgtgatgaa tttcgacaag gccgaaggct acctgatcgg cccgccgaac aagggcctgg
     5401 agtgcatgtt caccttcatg aactcggcgc gcgtgggcac ctccctgcag ggactgggcg
     5461 cggcagagct gtcgtaccag ggagcattgc catatgcgaa agagcgtctg tccatgcgct
     5521 ccctcagcgg caaaaaattc cccgacaaag tggccgatcc gctgatcgtg cacggcgacg
     5581 tgcgccgcat gctgctgacc cagaaagcct tcgctgaagg tggtcgcgcc atgatttatt
     5641 acgccgccaa attcaccgac cacctgatct tcggcaaaac ccaggaagag aaggacgaag
     5701 ccgacgacca gctgggct
//
LOCUS       DASUEG010000239         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_404400, whole genome shotgun sequence.
ACCESSION   DASUEG010000239 DASUEG010000000
VERSION     DASUEG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404400"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VFX11_04490"
     CDS             <1..1085
                     /locus_tag="VFX11_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4937910.1"
                     /translation="KTKMLVNGKLSDYIPNPTFDVVAAPGSTVEYLKGNNPEGKSFRD
                     FMIVERCIPEYRVKTEKRYQLLEEHGLCGTLMFPTLASVVEGHMYEDPDLCHAIIHSL
                     NQWMLDEWGFGEDGKFFATPVITLMDADKALEEARWIVSKGSKVVLMRPSFVPGIHGC
                     RGMGSAEFDPIYALLAANDIIIAFHNSDNGVHDMYERHQRSEEGHQGEYQPFKKTDPL
                     ELVMDLNQATVGHNLAGLVCHGVFDRHPNLKVCYVETGVAWLYPLWDRMEHVYKMAPQ
                     LFKRHPHDVIREHVWFHPHFEENLDRLVKLVGVDRIIFGSDWPHPEGLARPGDWLECL
                     DGMSEVDKDKIMRTNMLKLLKLENRP"
     gene            1082..2398
                     /locus_tag="VFX11_04495"
     CDS             1082..2398
                     /locus_tag="VFX11_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4937911.1"
                     /translation="MKMSDMVIVSVDDHISEPPDMFNRHLQGDDLATAPKFHTSPTGA
                     NYWTYQGMTMTSVGLNAVVGRVPEEYGMEPQSLEQVRKGCYDPKARIDDMNVNGIAAS
                     LNFGSVGGFDGSRCFHKAPDLTLAVKHLRAYNDWHILEWCGFDRSRFIPCALLPTWDM
                     SATVTEIKRIASLGCTSVTLSENPTRMGLPSIHNAYWEPMWKALAVHNITICLHIGSG
                     NPPPHASDETPIEAWITTMPMSISVGAADWLQLEALQRYPDLKIILSESGIGWVPYFM
                     ERADFSHSRHKAWTHSGYGGKKPSEVFRDHFMTCFIDDAFGLQNLGAIGEDNVAYECD
                     YPHSDALWPEVPEYLWKSLQHLTDTQIDKITHLNAMRWLHHDLFKHYKRDELTVGALR
                     AKAAADKVDITPISSGGAAPLAEGEVKRRVTSGDIFRMMAKQANVA"
     gene            2435..>2699
                     /locus_tag="VFX11_04500"
     CDS             2435..>2699
                     /locus_tag="VFX11_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4937912.1"
                     /translation="MPNAATLEATASLEKRLNATDITPLIASRVELSKQDLLSGHYAA
                     DLRELLERRGVLVFKEIKFTDAEQVAFTKTMGQFMPELEGQEIY"
BASE COUNT          626 a          757 c          789 g          527 t
ORIGIN      
        1 gcaaaaccaa aatgctggtg aacggcaagc tgtccgacta cattcccaat cccacctttg
       61 acgtggtggc agcgccgggc agcacagtgg aatatctgaa gggcaacaat cccgagggca
      121 aatcgttccg ggacttcatg attgtcgaac gttgcatccc ggaataccgg gtgaaaaccg
      181 aaaagcgcta ccagctgctg gaagagcatg gcctgtgtgg caccctgatg tttcccacgc
      241 tggcatcggt ggttgaaggc cacatgtatg aagatcctga cctgtgccac gccatcattc
      301 attccctgaa ccagtggatg ctggatgagt ggggttttgg tgaggacggc aagttttttg
      361 ccactccggt tatcaccctg atggatgccg acaaggccct ggaagaagcc aggtggattg
      421 tgtcaaaagg ctccaaagtc gtgttgatgc ggccctcctt cgtgccgggt attcatggct
      481 gccgtggcat gggatcggcg gaattcgatc cgatctacgc actgctggcg gccaacgaca
      541 tcatcattgc gtttcacaat tcggataacg gcgtgcacga catgtatgaa cgccaccagc
      601 gaagcgaaga aggtcatcag ggcgaatatc agccgttcaa aaagaccgac ccgctggaac
      661 tggtgatgga tctcaatcag gccaccgtgg gccacaacct ggcaggactg gtgtgtcacg
      721 gcgtgttcga ccgtcatccc aacctgaagg tgtgctacgt ggaaaccggt gtggcctggc
      781 tctacccgct gtgggatcgc atggagcacg tctacaaaat ggcaccccag ttattcaaac
      841 ggcatcccca cgatgtgatt cgcgaacacg tgtggttcca tccccatttc gaggaaaatc
      901 tcgaccgcct ggtgaagctg gtgggcgttg accgcatcat cttcggcagc gactggcccc
      961 atccggaagg cctggcgcgg ccaggggatt ggctggaatg tctggatggc atgagtgaag
     1021 tcgataagga caagatcatg cggaccaaca tgctgaagct tctgaaactg gagaaccggc
     1081 catgaagatg tcggacatgg tgattgtcag cgtcgacgat cacattagcg agccgcccga
     1141 tatgttcaac cggcatctcc agggcgacga tctggcaacg gcccccaaat tccataccag
     1201 ccccaccggt gccaattact ggacctacca gggcatgacc atgacatcgg tgggactcaa
     1261 tgccgtggtg ggacgggtgc cggaagaata cggcatggaa ccgcagtcgc tggaacaggt
     1321 gcgcaagggt tgctacgacc cgaaagcccg catcgatgac atgaacgtca acggcattgc
     1381 cgcctcgctg aactttggca gcgtgggcgg tttcgatggc agccgctgtt ttcacaaggc
     1441 gccggacctg acgctggccg ttaaacatct gcgcgcctac aacgactggc atatcctgga
     1501 atggtgcggc ttcgatcgca gccgcttcat tccctgcgcg ctgctgccga cctgggacat
     1561 gagcgcaacg gtgactgaga tcaagcgcat cgcttcgctg ggatgcacct ctgtcaccct
     1621 cagcgaaaat cccacccgca tgggtttgcc gagtatccac aacgcctatt gggagccgat
     1681 gtggaaagcg ctggccgtac acaacattac gatctgtctg catatcggct cgggcaatcc
     1741 gccgccccac gcatcagatg aaacaccgat cgaggcatgg atcaccacga tgcccatgtc
     1801 gatttccgtg ggcgcggcgg actggttgca actggaagcc ctgcaacgct atccggatct
     1861 gaaaatcatt ctgtcggaaa gcggtatcgg ctgggtgccc tacttcatgg agcgcgccga
     1921 tttcagtcat tcgcgccaca aggcgtggac gcactccgga tacggcggca aaaagcccag
     1981 cgaggttttc cgcgaccatt tcatgacctg cttcatcgac gatgcgtttg gcttgcagaa
     2041 cctcggcgcc atcggcgagg acaatgtggc ctacgaatgt gattaccccc actcggacgc
     2101 gctctggccg gaagttccgg aatatctgtg gaagagcctg caacatctga ccgacaccca
     2161 gattgacaag atcacgcatc tgaatgcgat gcgctggttg caccatgatc tgttcaaaca
     2221 ctacaaacgt gacgagctga cggtgggagc actgcgcgcc aaagccgcag ctgacaaggt
     2281 ggatatcacg ccgatttcca gtggtggcgc ggcgccgctg gcagagggcg aagtcaaacg
     2341 ccgggtcacc tcgggtgaca ttttccgcat gatggcaaag caggccaacg tggcctgagc
     2401 cattattttt ggtgaggaca acaggggatt caacatgccg aacgcagcaa cgctggaagc
     2461 aacagccagc ctggaaaaac gactgaacgc cacggatatc acgccgctga ttgccagccg
     2521 ggttgaactg agcaagcagg atctgctgag cggccattac gcggccgatc tgcgtgaact
     2581 gctcgaacgg cgcggcgtac tggtattcaa ggaaatcaaa ttcactgacg cggaacaggt
     2641 ggccttcacc aagacgatgg gccagttcat gcctgaactg gaagggcaag agatttaca
//
LOCUS       DASUEG010000240         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_844298, whole genome shotgun sequence.
ACCESSION   DASUEG010000240 DASUEG010000000
VERSION     DASUEG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844298"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1387)
                     /locus_tag="VFX11_04505"
     CDS             complement(122..1387)
                     /locus_tag="VFX11_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937913.1"
                     /translation="MRKVLIRLLAAFRRLSLWWLQLRGKTEQDVAAQRIVSGQSWDEF
                     CDTLKAAGAALNFPGAPRDPFNQAEGYRYLTRLARAGLMAFLEHADPRAPVLHRVVHE
                     TVKMGADNPDNFYQTACISGEYDYVIRGQRNTVKYLGFGTQIGHYGQGGGLPPSGYLE
                     SSAIELEADGSFLVHVSCKPHAKNWLPMQPQTGCLIVRQTFLDRTQEIPADLIIERVD
                     GDAIPTPLTPKQVDDGLKSASTLVAGAALLFAKWARDFQRHSNQLPLFDPAVSTTAGG
                     DPNIRYYHSHWALAPDEALLIEVTPPACEHWNFQLNNYWMESLDYVHFQIHTNKHLAH
                     YEKNGAVKIIVAHQDPGLPNWLSTAGHESGTMCFRWIKLQSPDSAPQPQTRVVNFSHI
                     ARSNAPHQTTVTQGKTRHEKENAGDLQSL"
     gene            complement(1398..2018)
                     /gene="cysC"
                     /locus_tag="VFX11_04510"
     CDS             complement(1398..2018)
                     /gene="cysC"
                     /locus_tag="VFX11_04510"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX4937914.1"
                     /translation="MTTHLHWHSGLISRADRARLLGHGGATLWFTGLSGSGKSTIAVA
                     LESALARQGKLSYRLDGDNIRLGINRNLGFSAADRTENIRRVGEVSKLFADAGVIVLS
                     SFISPYSADRELVRQLHEEADLPFLEIFVDCTLAEAERRDPKGLYKKARAGEIKNFTG
                     IDDPYEAPNKPDLHLHSHLQTLDDEIDTILTALQQRGILAQEALPA"
     gene            2242..>2766
                     /locus_tag="VFX11_04515"
     CDS             2242..>2766
                     /locus_tag="VFX11_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937915.1"
                     /translation="MSSANRRNRATENRGADSTRQRILDAAEELFATQTFSATSLREI
                     AARVGIREPSIYAHYAGKDAIYGEVIDRALQPFMAELDSWSQSELTLRELFDIPRKLL
                     YLHEQHPFRAQILHREFCNPPERISPKILVWLEQFAAKSAVFMSHLPENRMTRPDRHK
                     VIINIIALTNMTLGF"
BASE COUNT          510 a          742 c          900 g          614 t
ORIGIN      
        1 ggtcttgcgc cgcgcggcat tcatcagttg cgcaacgtcc aggtccgaca gcaggcccat
       61 tcgttccgcc acacggcctg cgcgattgaa cagggcaatc ggcaggggcc ggtagggttt
      121 gtcatagtga ttggaggtct ccagcgtttt ctttttcatg ccgagtcttg ccttgcgtaa
      181 cggtggtttg gtgtggtgcg ttgctgcgtg cgatgtggga gaaattcacc acgcgggttt
      241 gcggttgtgg cgcgctgtcg ggcgattgca gcttgatcca gcggaagcac atggtgccac
      301 tttcgtgccc tgccgtgctg agccagttcg gtagccccgg gtcctggtgg gcgacaatga
      361 ttttcactgc gccgttcttt tcgtagtgcg ccagatgttt gttggtatgg atctggaaat
      421 gcacgtagtc cagcgattcc atccagtagt tgttgagctg gaaattccag tgctcgcacg
      481 ccggcggagt gacttcgatc agcagcgctt catccggtgc cagcgcccag tggctgtggt
      541 aatagcggat gttgggatct ccgcccgcag tggtggacac ggcggggtcg aacagtggca
      601 gctggttgct gtgacgctgg aaatcccgcg cccatttggc aaacagcaag gcggcaccgg
      661 ctaccagtgt gctggcggat ttcaggccgt cgtccacctg cttgggcgtg agcggggtgg
      721 gaatggcgtc gccgtccacc cgttctatga tcagatccgc gggaatttcc tgcgtccggt
      781 ccagaaaggt ttgccgcacg atcaggcagc cggtttgcgg ttgcatcggc agccagtttt
      841 ttgcgtgcgg tttgcaactg acgtgaacca ggaaactgcc gtccgcttcg agttcgatgg
      901 cggaactttc caggtagccg gacggtggca aaccaccgcc ctggccgtaa tggccgatct
      961 gggtaccgaa gccaagatat ttcaccgtat tgcgctggcc acgaatcaca tagtcgtatt
     1021 cgccgctgat gcaggcggtc tggtaaaaat tgtcgggatt gtcggcgccc atttttacgg
     1081 tttcatgcac gacgcggtgc agcaccggtg cacgcggatc ggcgtgttcc agaaatgcca
     1141 tcagtccggc gcgggcgagg cgggtgaggt agcgatagcc ttccgcctgg ttgaacggat
     1201 cgcgcggtgc gccgggaaaa ttcagcgcgg cgccggcggc tttcaatgtg tcgcagaatt
     1261 cgtcccagga ctggccgctg acgatgcgtt gcgcggccac gtcctgttcg gttttgccgc
     1321 gcagttgcag ccaccacaat gacaggcggc gaaaggcggc cagcaggcgg atcagaacct
     1381 ttctcatagg ggcagcctca tgctggcagc gcttcctgcg cgaggatgcc gcgttgctgc
     1441 agcgcggtca gaatggtgtc gatttcgtcg tccagcgtct gcaggtgtga atgcaggtga
     1501 aggtcgggct tgttgggtgc ttcataggga tcgtcgatgc cggtgaagtt tttgatctcg
     1561 ccggcacggg cttttttgta caggcccttg ggatcgcgcc gctccgcttc cgccagcgtg
     1621 cagtcgacaa aaatttccag gaatggcagg tcggcttctt cgtgcagttg gcgcaccagt
     1681 tcgcggtcgg cgctgtaggg actgatgaag ctggacaaca cgatgacgcc cgcatccgcg
     1741 aacagtttcg atacctcgcc cacacggcga atgttttcgg tgcggtcggc agcggaaaaa
     1801 cccagattgc gattgatgcc gaggcggatg ttgtcgccgt cgagccggta actgagtttg
     1861 ccctggcggg cgagtgcgga ttccagtgcg acggcaatgg tgcttttgcc gctgccggac
     1921 agcccggtga accagagggt ggcgccgccg tggcccagca ggcgggcgcg gtcggcgcgg
     1981 gaaatcaggc cggaatgcca gtgcaggtgg gtggtcatgg gcgggttcct ccgattttag
     2041 cctatcgttt attaggttgt tttggtgaat ctatccaatg aaaatgcatt ttgcaagtca
     2101 attgcaaaat aaatgcgtat tgttttgact atcatatgat aggtttgtgg gctaggatta
     2161 ggcatcagcg gcgccccggg ctggcgccgg atcaatgccg caatgcaagc ggaacaactt
     2221 ggcgatcaat atggggtgaa gatgagcagt gccaaccgtc gcaacagggc cacggaaaat
     2281 aggggggcag attccacccg gcagcgcatt ctcgacgcgg ccgaggagtt gtttgccacc
     2341 cagacgttct cagccacttc gttgcgggaa atcgcggcac gggtgggtat tcgggagccc
     2401 agcatttatg cgcactacgc ggggaaggat gccatttatg gcgaggtaat cgatcgggcg
     2461 ctgcaaccgt tcatggcgga gcttgacagc tggagccagt ccgaactcac cttgcgcgag
     2521 ctgttcgata ttccgcgcaa actgctgtat ctgcatgagc agcacccgtt tcgcgcgcag
     2581 atcctgcatc gggaattctg caatccgccg gagcgcatca gcccgaagat actggtgtgg
     2641 ctggaacagt tcgcagcgaa atccgcagtg ttcatgagcc acctgccgga aaaccgcatg
     2701 acccgtcctg atcgtcacaa ggtgatcatc aacatcatcg cgctgaccaa tatgaccctg
     2761 ggattc
//
LOCUS       DASUEG010000241         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_421527, whole genome shotgun sequence.
ACCESSION   DASUEG010000241 DASUEG010000000
VERSION     DASUEG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421527"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFX11_04520"
     CDS             complement(<1..340)
                     /locus_tag="VFX11_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4937916.1"
                     /translation="MNICFIGGGNMATALIGGLQRQAAGQHQIGVVEPDADKCQQLAS
                     QFGVQAAWQLDLAAGSDVLVLAVKPQQLREVAYSLQQYIAPTTLVISIAAGIRLADLQ
                     RWLGGHARLVR"
     gene            complement(337..1065)
                     /locus_tag="VFX11_04525"
     CDS             complement(337..1065)
                     /locus_tag="VFX11_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX4937917.1"
                     /translation="MATTETALQAIQQRIRLACEAAGRTAQSVRLIAVSKTFPAADVR
                     ALAAAGQRAFGENYVQEAIAKMAELHDLALEWHFIGPLQSNKTRAIAEQCNWVHSLDR
                     LRIAQRLSDQRPLSMDSLRVLLQVNISGEASKSGCTPEELPALAAAVAQLPRLQLLGL
                     MTVPEPTPDVAVQRARFARLRQLRDSLQQQLGLALPELSMGMSADLEAAIGEGATLVR
                     VGTALFGARSYAPSSTSSEHKDPS"
     gene            1130..2170
                     /locus_tag="VFX11_04530"
     CDS             1130..2170
                     /locus_tag="VFX11_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX4937918.1"
                     /translation="MDISELLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMEH
                     KDVHDMLYDIMNDGQRKQYEETLECDFSFEIPNVARFRVNAFNQHRGAGAVFRTIPSK
                     VLTLEELNCPPIFKDIAQNPRGIVLVTGPTGSGKSTTLAAMINYINENDYGHILTVED
                     PIEFVHEAKKCLVNQRELGPHTLSFANALRAALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTVDRIVDVFPAAEKDMVRAMLSESLRAVISQTLLKTKDGQG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNIGMQTLDQCLTNLVNRGIVSAAE
                     ARTKAANKDNFN"
     gene            2204..>2944
                     /locus_tag="VFX11_04535"
     CDS             2204..>2944
                     /locus_tag="VFX11_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX4937919.1"
                     /translation="MERDQATRFVHDLLKLMVSKRASDLFLTADFPPALKIDGKVTPV
                     SNQGLTGQHTKELARALMNDRQSEEFEASRECNFAINPPGIGRFRCNAFVQQGRVGIV
                     LRVITTTIPQFDQLGLPDVLKDIAMTKRGLVMFVGGTGSGKSTSLAAMIGHRNQNSHG
                     HIITIEDPIEYVHEHQGCIITQREVGVDTDSWAHALKNTLRQAPDVILIGEIRERDTM
                     DYAIAFSETGHLCMSTLHANSANQALDRI"
BASE COUNT          643 a          895 c          841 g          565 t
ORIGIN      
        1 cccgcaccag gcgggcatgg ccgcccagcc agcgctgcag atctgccagc cggatgccgg
       61 cagcgatgga aatcaccagc gtggtcgggg caatgtattg ctgcaggctg taagccacct
      121 cgcgcagctg ctgcggcttg accgccagca ccagcacgtc actgccagcg gccagatcca
      181 gctgccaggc ggcctgcacg ccaaactggc tggccagctg ctgacacttg tcggcatccg
      241 gctccaccac cccgatctgg tgctgccccg ccgcctgccg ctgcaagccg ccgatcagtg
      301 ccgtagccat attgccgccg ccgataaaac agatgttcat gaaggatcct tgtgctcaga
      361 agaggtagaa gaaggggcat aactgcgtgc gccaaacagg gccgtgccca cccgcaccag
      421 ggtggcgccc tcgccaatgg cggcttccag gtcggccgac atgcccatgg acagctcggg
      481 caatgccagt cccagctgct gttgcaggct atcgcgcagc tggcgcagcc gggcaaagcg
      541 ggcgcgctgc acggctacat ccggcgtcgg ctcgggcacg gtcatcaggc ccagcagctg
      601 caggcgcggc aattgtgcca ctgcggcagc cagagccggc agttcttcgg gcgtacatcc
      661 cgatttactc gcctcaccac tgatattgac ctgtaataaa accctgagac tgtccatact
      721 gagaggacgc tggtccgata aacgctgcgc aatacgcagc cggtccagtg agtgcaccca
      781 gttgcattgc tcggcaatag cccgcgtttt gttactttgc agcggcccga tgaaatgcca
      841 ttccagtgcg aggtcgtgca gctcggccat tttggctatt gcttcctgca cgtaattctc
      901 gccaaatgcg cgttgcccgg ccgcggcgag tgcacgcaca tccgcggcgg gaaaggtctt
      961 ggaaactgca atcaggcgca cgctctgtgc tgtgcgcccg gcggcctcac aggccagccg
     1021 gatccgttgc tgtattgctt gcaatgccgt ttcggttgtt gccataatcg attgggacag
     1081 ccaataaaaa tggggcgaaa atcgccaccg atgcagagga taggattata tggacatttc
     1141 ggaactgtta gcgtttggcg taaaaaacaa agcctcggat ttgcaccttt ccgccggcct
     1201 gccacccatg atccgcgtgc acggtgatgt tcgccgcatc aatctgccgg ccatggagca
     1261 taaagacgta cacgatatgc tgtacgacat catgaacgac ggccagcgca agcagtatga
     1321 agaaacgctg gagtgtgact tttcgtttga aattccgaat gtcgcccgtt tccgtgtcaa
     1381 tgcgtttaac cagcatcggg gtgccggcgc ggtattccgg accattccgt ccaaggtgct
     1441 gacgctggaa gagctgaact gcccgcccat tttcaaggat attgcacaga atccgcgcgg
     1501 catcgtgctg gtcaccggtc cgaccggctc gggcaaatcc acgacgctgg ccgccatgat
     1561 caactacatc aatgaaaacg actatggtca catcctgacg gtggaagacc cgatcgaatt
     1621 cgtgcacgaa gctaaaaaat gtctggtcaa ccagcgtgag ctcggcccgc acaccctgtc
     1681 gttcgccaac gcactgcgcg ccgcgctgcg ggaagatccg gacgtgatcc tggtgggcga
     1741 aatgcgtgac ctggaaacca tccggctggc gctgaccgcg gccgaaaccg gtcacctggt
     1801 gtttggtacc ctgcacacgt cctcggccgc caaaacggtg gaccgtattg tggacgtatt
     1861 cccggcggca gaaaaagaca tggtgcgcgc catgttgtcc gaatcgctgc gcgcggttat
     1921 ttcgcagacc ctgctcaaga ccaaggacgg ccaggggcgt gtagctgccc acgaaatcat
     1981 gatcggcacg ccggctatcc gtaacctgat tcgtgaaaac aagatcgccc agatgtactc
     2041 gtcgattcag accggtcaga atatcggcat gcagacactg gatcagtgtc tgaccaatct
     2101 ggtcaatcgt ggcatcgtat cggcagccga agcgcgcacc aaagccgcca acaaggataa
     2161 tttcaactga taaacgcctg ttgacaggca ctggagagag gcaatggaac gcgatcaagc
     2221 cacacgcttt gtgcacgatc tgctcaagct tatggtcagc aaacgtgcgt ccgacctttt
     2281 cctgaccgcg gattttccgc cggccctgaa aatcgacggc aaggtaactc cggtttccaa
     2341 tcagggactc acgggccagc acaccaagga actggcccgg gcgctgatga atgaccggca
     2401 gagtgaagag tttgaagcca gccgggaatg taatttcgcc atcaatccgc ccggcattgg
     2461 ccgcttccgc tgcaacgcct ttgtgcagca ggggcgggtg ggcatcgtac tgcgcgtcat
     2521 taccacgacc attccacaat tcgatcagct gggtctgccc gatgtgctga aagatatcgc
     2581 catgaccaaa cgcggtctgg tgatgtttgt ggggggtacc ggctccggta aatcgacctc
     2641 gctggcagcg atgattggcc atcgcaatca gaacagccat ggccacatca ttaccatcga
     2701 agatccgatc gaatatgtgc acgaacacca gggctgcatc attacccagc gtgaagtggg
     2761 cgtagacacc gattcctggg cacatgcgct gaaaaacacc ctgcgtcagg cgcccgatgt
     2821 cattctgatc ggcgaaatcc gtgagcgcga taccatggac tacgccattg ccttctcgga
     2881 aaccggtcac ctgtgtatgt ctaccctgca cgccaacagc gccaatcagg cgctggaccg
     2941 tatc
//
LOCUS       DASUEG010000242         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2154940, whole genome shotgun sequence.
ACCESSION   DASUEG010000242 DASUEG010000000
VERSION     DASUEG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154940"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFX11_04540"
     CDS             complement(<1..251)
                     /locus_tag="VFX11_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4937920.1"
                     /translation="MSIPASFEGKLKLPVVAAPMFLVSGPQLVIETCKAGVVGTFPAL
                     NQRTSEGFQEWLVQIEQALRQHETATGTKCAPFGVNLIV"
     gene            369..1118
                     /locus_tag="VFX11_04545"
     CDS             369..1118
                     /locus_tag="VFX11_04545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4937921.1"
                     /translation="MAVLFHQQHNGHIYEVRGAGQTRRLYTDGVFHSQYNPQHGVTGS
                     VWDLLFLPSQFYDPAELKRVLVLGVGGGAAIHMLNRFAAPQAVVGVELNRIHLQVARK
                     FFGLRYRNLQLVHANAIDWLIGYDGEPFDLIIDDLYYEDQGLPTRAIAADASWFDVLL
                     EHLTDRGMLVLNFVGQKEWKQSAYFQESFVRDEFPSVYRFTTPTCHNAVIALLRQKKT
                     LRELRSRLRTHAELDERNARCRLRYKVSRIV"
     gene            complement(1102..>2038)
                     /locus_tag="VFX11_04550"
     CDS             complement(1102..>2038)
                     /locus_tag="VFX11_04550"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4937922.1"
                     /translation="LKAIPDLQILTNRQDIQQCAQQAGLSCVFNDMDLTPLDTGFDLI
                     AYRISKEKALVHHIINQSALHLSPQGELVLAGFKDEGTKTYISKAEQYFGCKAQIVRG
                     ERQLQIATLQPQRLGEPLDVRQYSELQLIAEQDGLRIFSKPGQYGWNKIDSGSAHLID
                     CLRQQLQDGLIKPATALDLGCGYGYLALHAARLGIAAITATDNNAAAVFSCRHNFTEN
                     RINGEVIADDCAASLSAQFDLVLCNPPFHRGFETEQALTEKFVVSAFRHLKKGGSALF
                     VINHFIPLDAVAAPWFRGIELLFRDKQFKVFKLSC"
BASE COUNT          411 a          584 c          595 g          448 t
ORIGIN      
        1 tgcacgatca ggttgacgcc gaacggtgcg cattttgtgc cggtggcagt ttcatgctgg
       61 cgcaatgcct gctcgatctg caccagccat tcctggaagc cttcgctggt gcgctggttc
      121 agcgccggaa aggtgccgac cacgccggcc ttgcaggttt caatcacgag ctgcgggccc
      181 gacaccagaa acatgggtgc ggcgacaacg ggcagtttca gttttccttc aaaggaagcg
      241 ggtatcgaca tggttcacct gaacgaaagg gttgcaaagt tctgcaagga taccatgagg
      301 cgcgccgccg ccccagtgag ctgctacaat gcgcgcactt ttggccgtag cacagggctt
      361 ttctcaccat ggcagtactg tttcaccagc agcacaacgg acacatctat gaagtgcgcg
      421 gcgctggaca aacccggcgc ctctacaccg acggcgtttt ccacagtcag tacaacccgc
      481 agcatggcgt taccggcagc gtgtgggatc tgctgtttct gcccagccag ttttacgacc
      541 cggcggaatt gaaacgcgtg ctggtgttgg gagtgggagg aggcgctgcg attcacatgc
      601 tgaaccgatt tgcagcacct caggccgttg taggcgtaga actgaatcgc attcacctgc
      661 aggtggcgcg gaagtttttc ggtctgcgct accgcaatct gcagctggtg catgccaatg
      721 ccatcgactg gctgatcgga tacgacggcg agccctttga tctcatcatc gatgatctgt
      781 attacgagga tcagggcctg cccacccgcg ccattgccgc cgatgccagc tggttcgatg
      841 tgctgctgga gcatctcacc gatcgcggca tgctggtgct gaattttgtc ggccagaagg
      901 aatggaagca gagcgcctat tttcaggaat cattcgtgcg cgatgaattt ccgtcggtgt
      961 accgtttcac cacgcccacc tgccacaacg ccgtcattgc actgttgcgg caaaaaaaga
     1021 ccctgcggga acttcgttcg cgcctgcgga cacacgctga actggatgaa cgcaacgccc
     1081 gctgccggct gcgctacaag gtcagcagga tagtttgaac accttgaact gcttgtcgcg
     1141 gaacagcagt tcgatgccgc gaaaccaggg cgcggccact gcgtccagtg gaatgaaatg
     1201 gttgatcacg aacagcgcgc tgccgccttt cttcagatgc cggaaggcgc tgacaacgaa
     1261 tttttccgtc aatgcctgtt ccgtttcgaa gccgcggtga aacggtggat tgcacagcac
     1321 cagatcgaat tgcgccgaca gactggcggc acagtcgtca gcgatgactt cgccatttat
     1381 gcggttttcc gtgaaattgt gacggcaact gaaaacagca gctgcgttat tgtcggtggc
     1441 agtaatggcg gcgatgccca gccgcgccgc atgcaacgcc agatagccat agccacaacc
     1501 cagatccagc gctgttgccg gtttgatcag accgtcctgc agttgctggc gcaggcagtc
     1561 gatcaggtgc gcgctgccgc tgtcgatctt gttccagcca tactggcccg gcttgctgaa
     1621 aatgcgcagg ccgtcctgct ctgcaatcag ctgcagttca ctgtattgac gcacatccag
     1681 cggctcgcca agtcgctgcg gttgcaatgt cgcaatttgc agctggcgtt caccccggac
     1741 aatctgtgcc ttgcaaccga aatattgttc ggccttgctg atgtaggtct tggtcccctc
     1801 gtccttgaag cccgccagca ccagttctcc ctgtggtgac agatgcaggg cggactggtt
     1861 gatgatgtgg tgcaccagcg ccttttcctt ggaaatgcga taggcgatca ggtcgaatcc
     1921 ggtatccagc ggcgtcagat ccatatcgtt gaaaacgcag gataatccgg cctgttgcgc
     1981 gcactgctgg atgtcctgcc ggttggtgag gatctgcagg tcaggaatgg ctttcagc
//
LOCUS       DASUEG010000243         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2003646, whole genome shotgun sequence.
ACCESSION   DASUEG010000243 DASUEG010000000
VERSION     DASUEG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003646"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1687)
                     /locus_tag="VFX11_04555"
     CDS             complement(14..1687)
                     /locus_tag="VFX11_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937923.1"
                     /translation="MKRIARLGSEQFSAADALAGHAELTQILKQHLPPSTVALFARPK
                     AVEGGVMEWYSELGGQPVPFSQLPPAEAAQVKRLLDERLDSIQHLAAKLDSQGADGQQ
                     QAVLLRQAARYPDTSTLYSLNGQPVLTFWGYGLRVPEPVPGAVNLNGVAPAGPEVPPP
                     VVDAVAAAEPERKRRRWWPWLLLALLLLGLLAALLWWLFCREPEPVAPPQEPPVVEQP
                     EEPKEEPVVEPEPPAEPEPEPQPEPEPEPEPVPPPPPPPPPPPPDPLDELAKKVSATK
                     DCAALQKMQAEEKLLQGNDPRAIALKQQIGATLNRDCKEQLIKQAKNLCPDQRPKELA
                     PELIIVFDASGSMDFSLNASREEIMRAEQMLRGMSNAGPGGQILGVLAGMATLEHLQR
                     EPKRITAAKQATTAVVQQVPRDVNIGLVMIERCPGARSAGYFSPAQRGSLMGQLQGIR
                     PVEGTPLADGVARAGQMLDGVNKESVMLVVSDGEESCGQDPCAVGKQLARTKPHLKIN
                     VVDIMGTGAGNCLAQATGGRVFTAKNVNELSVMTNQAAQDVLGPQNCRP"
     gene            complement(1719..>2168)
                     /locus_tag="VFX11_04560"
     CDS             complement(1719..>2168)
                     /locus_tag="VFX11_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937924.1"
                     /translation="VRKTPRDTNIGLVMIEQCPQARSAGYYSPAQRGSLTGLIQSLQP
                     QQGTPLGDGIAKGGQMLDGVNRASLMVVISDGQESCGADPCAIAKQLARTKPNLKINV
                     VDIMGTGAGTCVAQATGGRVFTAKSVNELNMMVTQAAQDVLGPANCP"
BASE COUNT          327 a          717 c          696 g          428 t
ORIGIN      
        1 gatccgcttt tcatcagggc ctgcagttct gcggcccgag cacatcctgc gccgcctggt
       61 tcgtcatcac cgacagttca ttcacattct tcgccgtgaa tacgcggcca ccggtggcct
      121 gggccaggca gttgccggcg ccggtgccca tgatgtccac cacgttgatt ttgaggtgcg
      181 gtttggtgcg ggccagctgc ttgccgacag cacacgggtc ctgaccgcag ctttcctcgc
      241 catccgacac caccagcatc accgattcct tgttgacgcc gtccagcatc tgcccggcac
      301 gggccacgcc gtcggccagc ggggtgcctt ccaccggacg gattccctgc agctgcccca
      361 tcaggctgcc gcgctgcgca ggtgaaaaat aaccggcact gcgggcaccg gggcagcgct
      421 cgatcatcac cagcccaatg ttcacgtcac ggggaacctg ctgcaccacg gcggtggtgg
      481 cctgcttggc ggcggtgatg cgcttgggtt cgcgctggag gtgctccagc gtcgccatgc
      541 ccgccagcac acccaggatc tggccgccgg ggccggcgtt gctcatgccg cgcagcatct
      601 gctctgcccg catgatttcc tcgcggctgg cattgaggct gaaatccatg gaaccggacg
      661 cgtcgaacac gatgatcagt tccggcgcca gttccttggg ccgctggtcc gggcacaggt
      721 ttttcgcctg cttgatcagc tgctccttgc agtcacggtt cagggtggcg ccgatctgct
      781 gtttcagggc aatcgcgcgc ggatcgttcc cctgcagcag tttttcttcg gcctgcattt
      841 tctgcagggc ggcgcagtcc ttggtggccg acactttttt cgccagctcg tccagcggat
      901 cgggtggtgg tgggggcggc ggcggtggag gcggtggaac cggttccggc tcgggctcag
      961 gttccggttg cggttcgggc tccggttcag caggaggctc gggctccacc acgggttctt
     1021 ccttcggctc ttccggctgt tcgaccaccg gcggttcctg agggggtgca accggttccg
     1081 gctcacggca gaacagccac cagagcaacg ccgcgagcag acccagcagc aacaatgcga
     1141 gcaacagcca gggccaccag cgacgacgct tgcgttccgg ttcggcggct gccactgcgt
     1201 ccaccacggg tggcggcacc tcggggccgg ctggagcgac accgttcagg ttgactgcac
     1261 ctggcaccgg ctcgggcacg cgcaggccat acccccagaa ggtgagcacc ggctggccgt
     1321 tgagggaata cagcgtgctg gtgtcgggat agcgtgccgc ctgccgcagc agcaccgcct
     1381 gttgttgtcc gtctgctccc tggctgtcca gcttggccgc caggtgctgg atcgaatcca
     1441 gccgttcgtc cagcaggcgt ttgacctgcg ccgcctcggc ggggggcaac tggctgaacg
     1501 gtaccggctg gccacccagc tcggagtacc attccatcac gccgccctcc accgctttcg
     1561 gcctggcgaa cagcgcgacg gtgctgggcg gcaaatgctg tttcaggatc tgcgtgagtt
     1621 cagcgtgtcc ggcgagcgcg tcggcggcac tgaactgttc cgatcccagg cgtgcaattc
     1681 gtttcatgcg ctagggcttc cgttcaaggc aatcagcatc aggggcaatt ggccgggccc
     1741 agcacatcct gggccgcctg tgtcaccatc atgttcagtt cattcacact cttggcggtg
     1801 aacacgcggc cgccggtagc ctgggccacg caggtgccgg cgccggtgcc catgatatcc
     1861 accacattga ttttcagatt cggtttggtg cgtgcaagct gtttggcgat cgcacaggga
     1921 tcagcaccgc agctttcctg gccatcggaa atcaccacca tcagcgaagc gcgattgacg
     1981 ccatccagca tctggccccc ttttgcaatg ccgtctccca gcggtgtgcc ctgctggggc
     2041 tggagagact ggatcagccc cgtgaggctg ccgcgctgcg ccggcgaata atagccggcg
     2101 gaacgggcct ggggacactg ttcaatcatc accaggccga tgttggtatc gcgcggtgtc
     2161 ttgcgcac
//
LOCUS       DASUEG010000244         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1926591, whole genome shotgun sequence.
ACCESSION   DASUEG010000244 DASUEG010000000
VERSION     DASUEG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926591"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFX11_04565"
     CDS             complement(<1..364)
                     /locus_tag="VFX11_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937925.1"
                     /translation="MPTKKSLKVKPKPRQPGRPKPEDVDAIESKLLEVGLKEFLEQGY
                     GNAAMARIAKAAGGSKTTLYSRYPSKEDLFRAIIFDQIERAAPQASLQSDAGPLSLEQ
                     GLKSYANHMLEHSLQDDMI"
     gene            complement(466..1188)
                     /locus_tag="VFX11_04570"
     CDS             complement(466..1188)
                     /locus_tag="VFX11_04570"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX4937926.1"
                     /translation="MHQSIEPSILYFGTPVIVVSSLNPGGSTNLAPISSAWWLGWSCM
                     LGFDASSQTVANLRRHGECVLNLAAEQNLPQVDKLALLTGSASVPLHKKALGSRYCRD
                     KFAAAGFTAQPSDVVLPDRVRECPIQLEARVSNVYGFGNDDPRMSVAACAIEVTVERI
                     HALPELCVDGRAHHVDPDKWRPLLMSFRHFYGLSARLQDSRLARGDEEQYAPWKQGGL
                     KGLVTNGVLRRVKRRGLIKNPE"
     gene            complement(1316..2170)
                     /locus_tag="VFX11_04575"
     CDS             complement(1316..2170)
                     /locus_tag="VFX11_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937927.1"
                     /translation="MPVLNLPTGPVHYTETGSGIPLVLLHANPGDSQDFAAVLPALAQ
                     RFRVLALDWPGYGQSPLPAHPESVHVDVFFQVLQDWLAQLQLPPAFFIGNSLGGYAAA
                     RLAAEAPERVRGLILVSPGGFTPHTAVTRAFCGLQGSRFALAPARWSGLYLKRRSATA
                     LAMLERAAGAQSQPSVRQLNRAIWRCFSLPCHDLREAAAAIKAPTLLMFGRYDPAIPA
                     DKDGVVAARCLPHADLVILPCGHAAFAELPESFLEYALPFLEQQAELTAADMPALDDV
                     RLQQKEVC"
BASE COUNT          516 a          763 c          723 g          466 t
ORIGIN      
        1 cgatcatgtc gtcctgcagg ctgtgttcga gcatgtgatt ggcatatgat ttcaaaccct
       61 gctcaagact gagcgggccc gcatcggatt gcagcgaggc ctgcggcgcc gcacgctcaa
      121 tctgatcgaa aataatggcg cggaacaaat cctctttcga cggatagcgg gaatagagcg
      181 tggtcttgga gcctcctgct gccttggcga ttcgcgccat ggcggcgttg ccatagccct
      241 gttccagaaa ctccttcagt ccgacttcca gcaatttgct ttcgatggca tccacatctt
      301 ccggtttcgg gcgcccgggc tgccggggtt tgggcttcac ttttaatgat tttttggtag
      361 gcattacggc ttggcacgct cttatggtca tcgaatctga acatcatagg ccagatggga
      421 accagcaggc gacccccgtc tttatccggt ggttttttga aaactttact cggggttctt
      481 gatcaggccc ctgcgcttga cccgccgcaa cacaccattg gtgacaagcc ctttcaaccc
      541 accctgcttc cagggagcat attgctcctc atcgccccgg gccagccgcg aatcctgcaa
      601 ccgcgccgac agcccgtaaa aatggcggaa gctcatcagt agtggccgcc acttgtcagg
      661 atcgacgtga tgcgcgcgac cgtccacgca caattccggc aacgcgtgta tccgttcgac
      721 cgtgacctcg atcgcgcagg ccgcgacgct catgcgtgga tcatcattgc caaatccata
      781 cacgttgctc acccgcgcct ccagttggat gggacattcg cgcacccgat ccggcagtac
      841 gacatctgaa ggttgcgcag tgaatccggc agcggcgaat ttgtcgcggc aataacggga
      901 accaagggct tttttatgca gtggcaccga cgcactgccg gtcagcagcg ccagtttgtc
      961 cacctgcggc agattctgtt ccgccgccag attcagcacg cactcgccgt gacgacgcag
     1021 gttggccacc gtctgcgacg aggcgtcaaa ccccagcatg cagctccagc ccagccacca
     1081 ggcggaggaa atgggcgcga ggttggtgct gccgccgggg ttgagcgaac tcaccacgat
     1141 caccggcgtg ccgaaataca gaatggaagg ttcgatggac tgatgcatga caagctctcc
     1201 gtgaaaaata aaatacacgg agagtgtggg tggtcgggcc aattcgcaac aacccggaac
     1261 ttgcggtatt gatcaacggc agattgttac agctgccaac atgcaacagc gccgttcagc
     1321 aaacttcttt ttgctgcaac cgcacatcgt ccagcgctgg catatccgcc gccgtcaatt
     1381 ccgcctgctg ctccagaaac ggcaacgcgt attccaggaa agattccggc agctctgcaa
     1441 acgcggcgtg gccgcagggc agaatcacca gatccgcatg gggcaggcag cgggccgcca
     1501 ctacaccatc cttgtccgca ggaatggcgg ggtcgtagcg gccgaacatc agcaaggtgg
     1561 gcgccttgat cgccgcggcg gcctcgcgca gatcatggca gggcagcgag aaacagcgcc
     1621 agatcgcccg gttcagctgt cgcacagacg gctgtgactg ggcgccggcc gcacgttcca
     1681 gcatggccag cgccgttgca gagcggcgtt tcagatacag gcccgaccag cgcgcaggag
     1741 ccagcgcaaa acggctgccc tgcagaccac aaaaggcgcg ggtcactgcg gtatggggcg
     1801 taaagccacc gggagatacc aggatcagcc cgcgcacccg ctctggcgct tccgctgcca
     1861 gccgcgctgc cgcgtagcca cccagggagt tgccgatgaa aaacgctggc ggcaattgca
     1921 gctgcgccag ccagtcctgc agtacctgga agaatacatc cacgtgcacg gattcgggat
     1981 gtgccggcaa cggcgactgg ccgtaaccgg gccagtccag cgccaacacc cggaaccgtt
     2041 gtgccagtgc gggcagcacg gcggcgaaat cctggctgtc gccgggattc gcgtgcagca
     2101 gcaccagggg aatgccactg ccggtttcag tgtagtgaac agggccggtg ggcagattca
     2161 gaacgggcat gaaacctccg ttgcgcgcaa tgtcattgtt attgtggagc cgggatgggc
     2221 gcgcaagcgc cattctggtc ccgccgctgc gccttggcaa cgtcatgcag tggcatcgtg
     2281 caaacaatgc gcggaaccgt ctcccgcagc ggcaggcagc aaaatgacat ttattggcaa
     2341 ctaaaccgga catcccctgc caaagatagc aggaggcagc ttcgccccgc atccgacagg
     2401 gagtgtccac aatgagcgta ctgttttttt ccatcactgt actggctctt ctttatctga
     2461 acacccgc
//
LOCUS       DASUEG010000245         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_910686, whole genome shotgun sequence.
ACCESSION   DASUEG010000245 DASUEG010000000
VERSION     DASUEG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910686"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFX11_04580"
     CDS             complement(<1..541)
                     /locus_tag="VFX11_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX4937928.1"
                     /translation="MLLQIPDVLTPDQVAEMRAALLAADWVDGRVTAGYQSAKAKQNL
                     QLPEDHPVARALGERIVQALSRHPLFVSAALPTKIYPPLFNRYEGGGNFGVHVDNAVR
                     DVRGTPHRVRTDLSATLFLCDPDEYDGGDLVIEDTYGAKSVKLPAGHMILYPGTSLHK
                     VTPVTRGARIASFFWIQSMI"
     gene            complement(597..>2101)
                     /locus_tag="VFX11_04585"
     CDS             complement(597..>2101)
                     /locus_tag="VFX11_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4937929.1"
                     /translation="NERIPDRGILWRRPTGEVGEPVPVDKSTYFGWANTDREEAVVDT
                     FTAVVEHDFSKQVSIRNTTRLARTDNFSSLATMNGLVCIDGEPFNATGTCPVAGGDTF
                     SMTGVPGNIRDDLTKVMANITDVTWKFTTGGIAHTLVTGFALTEEDFEREGWTARTPT
                     NGALPGSVVRDLNDPDPTYDIPFRYFRGGNQVEADVSNRALYVFDTMEFGAHWQLNAG
                     VRYERNEASYETTNITVASGAVASVSEAEVGDDLVSSRIGVVYKPVEHGSIYAAVGNS
                     LTPSATSVVTACTATGNNTYCGLDPEETTSYEVGTKWDLFDSRLSLSGALFRNERNSY
                     RVNSADTSEPQKLDGENYVEGIELGASGKITDQWAVYAGYSFMDSAVLQGAADEALTD
                     TQKDYEVPNTPRHTGNVWTTYLLPGGIELGYGLRHIGSYKTEAAAVTATVPRSTVHNA
                     MIGYRINRNVFARLNVNNLNDETYWTSVRAHGWGHPGEGRSAVLSVDVTL"
BASE COUNT          412 a          659 c          599 g          431 t
ORIGIN      
        1 gaatcatgct ctgtatccag aagaacgagg caatccgtgc accgcgcgtt actggcgtca
       61 ccttgtgcag gctggtgccc ggatacagga tcatgtggcc ggccggcagc tttacgcttt
      121 tggcaccgta ggtgtcctcg atgaccagat cgccaccgtc gtactcatcg ggatcacaca
      181 gaaaaagtgt tgctgacaga tcagtgcgta cccgatgcgg cgtaccccgt acatcacgta
      241 cggcattgtc gacatgcaca ccgaagttcc cgccgccttc gtaccgattg aacaagggcg
      301 gatagatttt ggtgggcagg gccgccgaca cgaacaacgg atggcgactg agcgcctgca
      361 caatgcgctc gcccagcgca cgggctaccg gatgatcctc aggcaattgc agattctgct
      421 ttgcctttgc cgactggtat ccggccgtga cacggccatc cacccagtcg gccgccagca
      481 aggcagcacg catctccgcc acctggtccg gcgtcagtac atcaggaatc tgcaacagca
      541 tgaaaatctc cggtcgcctg ccggtgcatc cgctgtacgc ggcaggcgtg caagcgtcag
      601 agtgtcacat ccacggacag cacggctgaa cgtccttcac ccgggtggcc ccaaccgtgc
      661 gcgcgcacgg atgtccagta ggtttcgtcg ttcaggttgt tcacattgag gcgggcaaac
      721 acgttgcggt tgatgcgata cccgatcatg gcgttatgca cggtgctgcg cggcaccgtt
      781 gcggtaaccg ccgcggcctc cgtcttgtag ctgccgatgt ggcgcaggcc gtagcccagc
      841 tctataccgc ccggcagcag gtaagtcgtc cacacgttgc cggtgtggcg cggcgtgttg
      901 ggaacttcat aatccttttg cgtatcggtc agcgcctcat cagccgcgcc ctgcagcaca
      961 gcgctgtcca tgaacgaata gccggcgtac acagcccact gatcggtgat cttgccggaa
     1021 gcgcccagct cgatgccttc cacataattc tcgccatcca gcttctgcgg ctcgctggtg
     1081 tcggcgctgt tcacgcgata gctattgcgc tcgttgcgga acaacgcacc gctcaatgac
     1141 aggcggctgt caaacagatc ccatttggta ccgacttcat agctggtggt ttcttccggg
     1201 tccaggccgc agtacgtgtt gttaccggta gccgtacagg ccgtcacgac agacgtggcg
     1261 gatggagtca gcgaattgcc cacggctgca taaatgctgc cgtgttcgac cggcttgtag
     1321 acgacaccga tacggctcga caccagatcg tcgcccacct ctgcctcgct cacggacgcg
     1381 acagcaccgg aggccaccgt gatgttcgtg gtctcataac tggcctcgtt acgctcataa
     1441 cgcacgcccg cattcagctg ccagtgcgca ccgaattcca tggtgtcgaa cacataaagg
     1501 gcccgattgc tgacgtcggc ctctacctga ttgcctccgc ggaaatagcg gaacggaatg
     1561 tcgtaggtgg ggtccggatc attcaggtcg cgcacgaccg agcccggcaa ggcgccattg
     1621 gtgggcgtac gtgcggtcca gccttcgcgc tcgaaatctt cctcggtcaa cgcaaaaccg
     1681 gtgaccagcg tatgggcaat cccacccgta gtgaatttcc atgtcacgtc cgtgatgttc
     1741 gccatcactt tggtcaggtc atctcgaatg ttgccaggaa cacccgtcat gctgaacgta
     1801 tcaccacctg ccacggggca cgtgccggtt gcgttgaaag gttcgccatc aatgcagaca
     1861 agcccgttca tcgttgccag gctggagaaa ttatcagttc gcgcaagacg cgtggtgtta
     1921 cgaatcgaca cctgcttgct gaagtcatgc tccactacgg cggtgaaggt atccaccacg
     1981 gcctcttcac gatcagtatt ggcccagccg aaataggtgc tcttgtcgac cggcaccggc
     2041 tcgcccacct ccccggtcgg acgacgccag agaatgccgc gatccggaat gcgttcgttt
     2101 t
//
LOCUS       DASUEG010000246         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1926579, whole genome shotgun sequence.
ACCESSION   DASUEG010000246 DASUEG010000000
VERSION     DASUEG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926579"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFX11_04590"
     CDS             <1..596
                     /locus_tag="VFX11_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4937930.1"
                     /translation="LPDCRMPLQIFEKRYLDMVSHCMRTNTGFCVALLRPGSERHEVN
                     RPEQTPAGTGLPFYPLGTEARIVDFGQRENGLLSITILGGQRLALTDVRQAADGLWMA
                     SAEPRPEQADLNITDRGEWTALLTRLLEMTGLGALGIDADAQRSEQLMNYLIMLLPMP
                     AEVKQDLLDTDSHAARWEKLSGAMALLSGQDHRQTRN"
     gene            complement(627..2690)
                     /gene="glgX"
                     /locus_tag="VFX11_04595"
     CDS             complement(627..2690)
                     /gene="glgX"
                     /locus_tag="VFX11_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX4937931.1"
                     /translation="MPNAIQTVPFTFSAGQHLPPGAQPDASGVNFAIFSRDAVAVELC
                     LYTDGDAEPLQIIRLDPRQHRTFYFWHVHVEGAQPGLRYTWRVAHAGQDLETAPELLD
                     PWARAVDDAHWNRRATMAGELGHALRAIVTTRDERDPGLRLVSCDLSDAIIYEVHVGG
                     FTRHPSAGVQHPGTFAALIEKIPYLQALGITHVELLPVMAFDEQDVPPGAVANGLGNY
                     WGYSTCGFYAPHPRYCVDPARAPQEFRACVRALHEAGIGVLMDVVFNHTAEGGKEGPV
                     INFKALANDIFYHLDPHDRSRYRDYTGCGNTVNCNHPLVTNFIVHCLEYWAASLGVDG
                     FRFDLASVFARDENGNVMANPLLPWSIELSRVLIERPLIAEAWDAAGLYQVGAFPGMH
                     WSEWNGRYRDLVRRFVRGDHGLIGELATRLTGSSDLYGPSNRLPANGVNFVTCHDGFT
                     LQDLVSYNSKRNEANGENNRDGSDDNLSWNCGAEGDTDDATVLELRRRQARNVFAILL
                     LSQGVPMLLAGDEVLHSQRGNNNAYCQDNELSWFDWRRQESQADMLRFVRELIALRKR
                     HPSLRARRFLTGELQSGTDMPDIRWHGRELDKPLWHDPSAQLLAFTLAPAQPDEPPLH
                     VVLNMADACCTVELPSLPGLHWHRALDTARTSPDDILPPSQQPVLATDSVELQPRSVL
                     VLEAR"
     gene            complement(2700..>2920)
                     /locus_tag="VFX11_04600"
     CDS             complement(2700..>2920)
                     /locus_tag="VFX11_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX4937932.1"
                     /translation="QVRQLHDTPLIFVGPMWRGLVEWASAQMLRPGFELASPDDMKIP
                     RCVDPAEEAIAIVREHQARWKAVQAGSQ"
BASE COUNT          583 a          930 c          906 g          501 t
ORIGIN      
        1 tgctgccgga ctgcaggatg ccgctgcaga ttttcgaaaa acgctatctg gacatggtgt
       61 cgcactgcat gcgcaccaac accggttttt gcgtggcact gctgcggccc ggatcggaac
      121 gccacgaagt gaaccgtccg gaacagacgc cggccggcac cggattgccg ttctacccgc
      181 tgggcaccga ggcccgcatc gtggatttcg gccagcggga aaatggtctg ctgtcgatca
      241 ccattctggg cggccaacgc ctggcgttga cggacgtgcg ccaggccgcc gacggactgt
      301 ggatggccag cgcggagccc cggcccgagc aggccgatct caacattacc gaccgcggag
      361 agtggaccgc cctgctgacg cggctgctgg agatgaccgg cctgggcgca ctgggcatcg
      421 acgccgatgc gcaacgcagc gagcaactga tgaattacct gatcatgctg ctgcccatgc
      481 cggcggaggt gaaacaggac ttgctggata ccgactccca cgctgcccgc tgggaaaagc
      541 tgtccggcgc gatggccctg ctgagcggac aggaccatcg ccagacgcgg aactgacaga
      601 gacgtaaccg ataagagccg cgaaaatcag cgcgcctcca gtaccagcac gctgcggggt
      661 tgtagctcaa cggaatcggt cgccagcacg ggctgttgcg acggcggcag aatgtcgtca
      721 ggggacgtgc gcgcagtgtc aagcgcgcgg tgccagtgca aaccgggcag cgacggtaac
      781 tcgaccgtgc agcatgcgtc ggccatattc aataccacat gcagtggtgg ttcatcaggc
      841 tgtgccggcg ccagcgtgaa ggccagcagc tgcgcggatg gatcgtgcca caaaggttta
      901 tccagttcgc ggccatgcca gcggatatcc ggcatatcgg tgccagactg cagttcaccc
      961 gtgagaaagc gacgcgcgcg caacgaggga tgccgtttgc gtaatgcaat cagctcgcgc
     1021 acaaaacgca gcatatcggc ctgggattcc tgccggcgcc agtcgaacca gctcagctcg
     1081 ttgtcctggc agtaggcgtt gttgttgccg cgctggctgt gcagcacttc atcgcccgcc
     1141 agcagcatgg gcacgccctg gctcagcagc agaatggcaa atacattgcg cgcctggcgc
     1201 cggcgcaatt ccagtacggt ggcatcgtcg gtgtcacctt ctgcgccgca attccagctc
     1261 aggttgtcgt cgctgccatc acgattgttc tcaccgttgg cttcgttgcg cttgctgttg
     1321 tagctcacca gatcctgcag cgtaaaacca tcgtggcagg tgacgaagtt gacgccgttg
     1381 gccggcagcc ggttggacgg cccgtataaa tcgctggaac cggtgagccg ggtggccagc
     1441 tccccaatca gcccgtggtc accgcgcaca aaacgccgca ccagatcgcg gtagcggccg
     1501 ttccattccg accagtgcat gccgggaaac gcgcccacct gatataaacc ggcggcatcc
     1561 caggcttccg caatcagcgg ccgctcgatc aaaacgcgcg acagttcgat actccacggc
     1621 agcagcggat tcgccatcac attgccgttt tcatcgcgcg cgaacacgct ggccagatca
     1681 aagcggaagc catcgacacc cagactcgcc gcccagtatt ccaggcagtg gacgataaaa
     1741 ttcgtcacca gcggatggtt gcagttcacg gtgttgccgc agccggtgta atcacggtag
     1801 cggctgcggt cgtgcggatc gagatgatag aaaatatcgt tggccagcgc cttgaaattg
     1861 atgaccgggc cttccttgcc gccctcggcc gtgtgattga acaccacgtc catcaacacg
     1921 ccaatgccgg cttcatgcag cgcccgcacg caggcgcgga attcctgcgg tgcccgggca
     1981 ggatcgacac aatagcgcgg atgcggcgca taaaaaccgc aggtgctgta accccagtaa
     2041 ttgccgaggc cgttcgccac cgcgcccggc ggcacgtcct gttcatcgaa agccatcacg
     2101 ggcaacaatt ccacgtgcgt gatgccgagc gcctgcagat agggaatttt ttcgatcagc
     2161 gcagcaaagg tgccggggtg ttgcactccc gcagacgggt gccgcgtgaa gccaccaaca
     2221 tgcacttcat aaatgatggc gtcgctcaga tcacaggaca ccagccgcaa gccgggatcg
     2281 cgctcatcgc gcgtggtgac gatagcgcgc aaggcatggc ccagttcacc cgccatggtg
     2341 gcacggcgat tccagtgggc gtcgtccacc gcccgcgccc aggggtccag caattccgga
     2401 gcggtttcca gatcctgccc cgcgtgagcg acgcgccagg tgtaacgcaa cccgggctgc
     2461 gcaccttcca catggacatg ccagaaatag aacgtgcgat gctggcgcgg atcgagccgg
     2521 atgatttgca gcggttcggc gtcaccatcg gtgtacagac acagttccac ggcaacggca
     2581 tcgcgggaaa aaatcgcgaa gttgacgccg gatgcatccg gctgcgcgcc cggcggcaga
     2641 tgttgcccgg cgctgaaggt gaacggaact gtctggatcg cgttcggcat accatcgcct
     2701 cactggctac cggcctgcac cgctttccag cgggcctgat gctcgcggac gatggcgatg
     2761 gcctcctccg ccggatctac acagcggggg attttcatgt cgtcggggct ggccagttca
     2821 aagcccggcc gcagcatctg cgcactggcc cattccacca gaccgcgcca catggggccg
     2881 acaaaaatca gcggcgtgtc gtgcagctgc cgcacctgca
//
LOCUS       DASUEG010000247         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1513803, whole genome shotgun sequence.
ACCESSION   DASUEG010000247 DASUEG010000000
VERSION     DASUEG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513803"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2624)
                     /locus_tag="VFX11_04605"
     CDS             complement(<1..>2624)
                     /locus_tag="VFX11_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX4937933.1"
                     /translation="IADLKVINPFDFFVEDYASKFPFEYEKQLAKELVPYLEIKEQGP
                     LLLDWIGTIERTPRTIVDFLVEVNRALFQHIEYSIRMEPGVQTCEETLTSRIGSCRDS
                     AFLLVQVLRHLGLAARFVSGYLVQLKPDIESLDGPSGPHADFTDLHAWCEVYVPGAGW
                     IGLDPTSGLFAGEGHIPLACTPDPVSAAPVTGATSKCEVEFEFENRVDRILEDPRVTK
                     PYTEEQWQNIQALGNQVEEDLVNGDVRLTMGGEPTFVSVDDMEGAEWNVTADSPNKRK
                     LAHSLFLGMQKHFAPGGVKHYGQGKWYPGEPLPRWQYACYWRKDGNPLWHNPALMADP
                     NHNYNFSVHNARQFMHQLCHELKLPERFAIPAYEDAVYYLWQEGNLPAEFDPTKKDLK
                     LDLERKRLLAQLQRGLDQPVGYTLPLNWDWYDRTWHSCSWTFRRGHLHLVPGDSPLGM
                     RLPLEVIHWLPPERREQHQPMSLFEEAGALPYFPPNLAPLEASETSEKNEKEKFIQTA
                     LCIEPRHGVLYIFLPPLSNADQFILLINAIESAAAKLSMPVVLEGYDPPKDLRLEKFM
                     VTPDPGVIEVNIHPAHSWKQLQQNTLDLYEIARKSRLGTEKFMVDGRHAGTGGGNHVT
                     MGGATPADSPMLRRPDVLRSLITFWQHHPGLSYLFSGLFIGPTSQAPRVDEGRNENLY
                     ELEIAFGQMPEGDVVQPWLVDRLLRNLLVDISGNTHRSEFCIDKLYSPDSAAGRLGLL
                     EFRAFEMPPHARMSLVQMLLLRTLVALFWKQPYRQRLVRWGTELHDRFLLPHFVASDL
                     NDVTETMQRWGIPFAMDWLAPFLEFRFPRYGAVQIGDAELALRFAIEPWHVMGEEVTR
                     SGTARFVDSSIERMQVKV"
BASE COUNT          506 a          795 c          796 g          527 t
ORIGIN      
        1 caccttcacc tgcatgcgtt cgatggatga atccacgaag cgcgcggtgc cggagcgcgt
       61 cacttcctca cccatcacat gccagggctc gatcgcgaag cgtagcgcca attccgcatc
      121 gccgatttgt accgctccgt aacgcggaaa acggaattcc agaaacggcg ccagccaatc
      181 catggcgaac ggaattcccc agcgctgcat cgtttcggtc acatcgttta agtcgcttgc
      241 gacaaaatgg ggcagcagga agcgatcgtg cagttcggtg ccccagcgca ccagtcgctg
      301 cctgtacggc tgtttccaga acagggccac cagcgtgcgc agcaacagca tctgcaccag
      361 gctcatgcgg gcatggggcg gcatctcgaa ggcgcggaat tccagcaggc caagtcggcc
      421 ggcagcggag tctggcgaat acagtttgtc gatgcagaat tccgagcggt gcgtgttgcc
      481 ggaaatgtcc accagcagat tgcgcagcag acggtccacc agccagggtt gcaccacatc
      541 accttccggc atctggccaa acgcaatttc cagttcatac aggttttcat tgcggccttc
      601 gtcgacgcgg ggggcctgcg aggtgggacc gatgaataac ccggagaaca ggtaggacag
      661 accgggatga tgctgccaga aggtgatcag actgcgcaac acatccgggc gccgcagcat
      721 cggcgaatcc gccggcgtgg caccgcccat ggtgacgtgg ttgccgccgc cggtaccggc
      781 atgacggcca tccaccatga acttttcggt gcccaaccgg gatttgcgcg cgatctcgta
      841 caggtccagc gtgttctgct gcagctgttt ccagctgtgg gctggatgga tgttcacttc
      901 gatcacaccc gggtccggcg tcaccatgaa tttttccagg cgcagatctt tgggtggatc
      961 gtagccttcc agcacgaccg gcatgctgag tttggcggcg gcggattcaa tggcattgat
     1021 cagcaggatg aactggtcgg cgttggacag gggcggcagg aaaatgtaca gcacgccgtg
     1081 gcgcggctcg atacagagcg cggtctggat gaatttttcc ttttcatttt tttcggacgt
     1141 ttcactggct tcgagtggcg ccagattggg cgggaaatag ggcagcgcac ccgcttcctc
     1201 gaacagactc ataggctggt gctgttcgcg ccgttccggc ggcagccagt ggatcacttc
     1261 cagcggcagc cgcatgccca atggtgaatc gccgggcacc aggtgcagat ggccccggcg
     1321 gaaggtccag ctgcagctgt gccaggtgcg gtcgtaccag tcccagttca gcggcagggt
     1381 gtatcccacc ggctggtcca gcccgcgctg caattgcgcc agcaggcgtt tgcgttccag
     1441 atccagcttc aggtcttttt ttgtgggatc gaattccgcc ggcaggttgc cttcctgcca
     1501 caggtaatac acggcgtctt cgtaggcggg aatcgcgaaa cgttccggca gcttcagctc
     1561 atgacacagc tggtgcatga actgccgcgc gttgtgcacg gaaaaattgt agttgtggtt
     1621 gggatcggcc atcagcgccg gattgtgcca cagcggattg ccgtccttgc gccagtagca
     1681 cgcatactgc cagcgcggca aaggctcgcc cgggtaccat ttgccctgac cgtaatgctt
     1741 cacgccgccg ggtgcgaagt gtttctgcat gccgagaaaa agactgtgcg ccagtttgcg
     1801 cttgttgggg ctgtcggcgg tgacgttcca ctcggcgcct tccatgtcgt ccaccgacac
     1861 aaaggtgggt tcgccaccca tggtcaggcg cacatcgcca ttcaccagat cctcttccac
     1921 ctggttgccc agcgcctgga tgttctgcca ctgttcttcg gtgtagggtt tggtgacgcg
     1981 cggatcttcc agaatgcggt cgacgcggtt ttcaaattca aactccactt cacacttgga
     2041 cgtggcaccg gtgaccggcg cggcgctcac cgggtccggc gtgcacgcga gcggaatgtg
     2101 gccttcaccg gcaaacaagc ctgaagtagg atcaagcccg atccagccgg cgcccggcac
     2161 atacacttcg caccaggcgt gcaggtcggt gaaatccgcg tgagggccac tggggccgtc
     2221 gagagattcg atatccggtt tcagctgcac caggtagccc gacacgaacc gcgccgccag
     2281 ccccagatgc cgcagcacct gcaccagcag gaatgcggaa tcgcggcagg agccgatgcg
     2341 actggtgagc gtttcctcgc aggtctgcac gcccggctcc atgcggatgc tgtattcgat
     2401 gtgctggaac agcgcacggt tcacctccac cagaaaatcc acgatggtgc gcggcgtgcg
     2461 ctcgatcgtg ccgatccagt ccagcagcag cggcccctgc tcttttattt ccagataagg
     2521 caccagttcc ttcgccagct gtttctcata ctcaaagggg aacttgctgg cgtaatcctc
     2581 cacgaagaaa tcgaacgggt tgatcacctt cagatcggcg atca
//
LOCUS       DASUEG010000248         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_314014, whole genome shotgun sequence.
ACCESSION   DASUEG010000248 DASUEG010000000
VERSION     DASUEG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314014"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFX11_04610"
     CDS             <1..248
                     /locus_tag="VFX11_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937934.1"
                     /translation="YGSNNSQMGTFSNLGVWNWDTSAAPYLLFTPPCTRTAPIGASAA
                     TFNGCLTLTLALHPFLRPDETMLQNLLQGWVDRLMQN"
     gene            complement(267..1277)
                     /locus_tag="VFX11_04615"
     CDS             complement(267..1277)
                     /locus_tag="VFX11_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX4937935.1"
                     /translation="MSVDYIKLPDHSTQNGSVSLQFPRLFFIAATLSITSPGLIAMEE
                     SIVSTFLIPGVLAAMMVGMGMTLQPLDFQRLYLYPKAVITGLLAQVLLLPALGFLIVS
                     LVPMAPELAVGLMIIALCPGGVGSNLLTLLARADSALSITLTAISNVLLVITLPMFIN
                     FFLRHFMGQGTDIQLPVLQTILSTALLTVLPIAVGMALRRYAGDLSGRMETGFRRLSV
                     AVLVLLMLGIGWRNREVFLNSLLDLGPAVLALNLGSMALGFALAALVRLELSQRVTIA
                     IETGFQNAALAILVTTTFLHNPQMALAPAFYGGIMFIGGFLLIGLLKLKAPPATASAT
                     TG"
     gene            1298..2839
                     /locus_tag="VFX11_04620"
     CDS             1298..2839
                     /locus_tag="VFX11_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4937936.1"
                     /translation="MNIYHDTFARHLLSAPDAPALCVQGSTFSYRDLECWVNGCRIAL
                     RDCGVQTGDPVAVLASRSLVAYVAILACAAEGFTWVPLNPAFPDARNQRMLTLSAARV
                     LVAGREAADQSQRLAEDANLQAVLAPEEAVPDARRFAPSDQVLPLVRFAADLPAYLLF
                     TSGTTGVPKGIPVSVGNLLAYLQNFHALYPLPAGLRYSQMFDLTFDLSVHDLFVCWQQ
                     GGCLVIPTAQDLLMPLQFVRRQQIQVWFSVPSMASIAKQSGLLRPGVLPSLQFAFFCG
                     EALPTASALAWMDAAPNARVANLYGPTEATIAITAFELQRDRDDWRQLSVVPIGWPYP
                     GQQARVVREDATLAAPGESGELWLAGTQLSAGYLADAAKTAAVFVQQRFAGCDETRWY
                     RTGDCATIHPDFGILYAGRLDRQVKINGYRVELQEVEHCLRQVAGTQLAAVVPWPRSA
                     DGLFTGVVGFVAQAWQTEDQVLAQLTATLPAYMQPRQIISLERLPVNANGKVDYGALQ
                     AMLAQ"
     gene            3020..3823
                     /gene="lgt"
                     /locus_tag="VFX11_04625"
     CDS             3020..3823
                     /gene="lgt"
                     /locus_tag="VFX11_04625"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4937937.1"
                     /translation="MLRFPEIDPVAISIGPLDIRWYGLMYLAGFVSGFMLLNRRARRP
                     QSGWTTEQVSDLIFYGALGVILGGRIGYVLFYNFGAFLNNPLTLFQVWNGGMSFHGGL
                     LGVMCAVLLFARNMQKNYWDVLDFGAPIVPIGLGLGRIGNFIGGELVGRPTDGPWGMV
                     FPHVDNLARHPSQLYQATLEGLALFSILWFFSRKPRPRYAVSGLFALFYGLFRFIVEF
                     ARQPDVQLGFVAFDWLTMGQLLSLPLIAVGLTLLYLAYGRHSSYSNSKI"
     gene            3839..>4575
                     /locus_tag="VFX11_04630"
     CDS             3839..>4575
                     /locus_tag="VFX11_04630"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX4937938.1"
                     /translation="MKQYLDLLRHIKENGVTKSDRTGTGTISLFGYQMRFNLADGFPL
                     VTTKKVHLKSIIHELLWFVRGETNIAYLKENGVSIWDEWADANGELGPVYGKQWRSWE
                     GPNGETVDQLQQLVDEIKRNPDSRRLVLSAWNPVVLPDVKFSPKENAAAGKQALPPCH
                     CLFQFYVVEGRLSCQLYQRSGDVFLGVPFNIASYALLTLMVAQVCGLKPGEFVHTLGD
                     THLYLNHLEQAREQLARLPRTLPKIRL"
BASE COUNT          854 a         1382 c         1431 g          908 t
ORIGIN      
        1 attacggcag caacaacagc cagatgggta ctttttcgaa cctgggtgtg tggaactggg
       61 ataccagtgc agcgccctat ctattgttta cgccaccttg cacacgcacg gcaccgattg
      121 gcgcatcggc tgccacgttc aatggttgcc tgacgctgac gctcgcgctg cacccgttcc
      181 tgcgtcccga tgaaaccatg ctgcagaatc tgttgcaggg ctgggtggat cggttgatgc
      241 agaactgagt ggcggctggc cgtagctcag ccagtggtgg cactggccgt tgccggtggg
      301 gctttcagtt tcagcagacc gatcagcaga aagccgccga tgaacataat gccgccgtaa
      361 aaggccggtg ccagcgccat ctgcggatta tgcaggaacg tggtggtcac caggatggcc
      421 agcgccgcat tctggaagcc ggtttcaatg gcgatggtga cgcgttgcga caattccagc
      481 cggaccagtg cggccagcgc aaagcccagc gccatggagc ccagattgag ggccagcacg
      541 gcgggtccca gatccagcag cgaattcagg aacacctcgc ggttgcgcca gccgatgccc
      601 agcatcaaca acaccagtac ggccacagac agccggcgaa agcccgtttc catgcgtcct
      661 gacagatcgc cggcatagcg cctcagcgcc atccccaccg caatgggcag aacggtgagc
      721 agcgcagtgg acaggatggt ctgcaatacc ggcagctgga tgtcggtacc ctggcccatg
      781 aaatggcgca ggaaaaaatt gatgaacatc ggcagcgtga tcaccagcag cacattgctg
      841 atggccgtga gtgtaatgga cagtgcactg tcggcccggg ccagcagggt cagcagattg
      901 gagccgactc cgccgggaca caacgcaatg atcatcagcc ccacggccag ttccggcgcc
      961 atcggcacca gtgacacgat caggaaaccc agcgccggca atagcagcac ctgcgccagc
     1021 aggcctgtga taacggcttt cggatacaga tacaggcgct ggaaatccag cggttgcagc
     1081 gtcatgccca tgcccaccat catggcggcg agcacacctg gaatcagaaa agtgctgaca
     1141 attgattctt ccatcgcaat caggcccggt gaagtgatcg acaaggttgc agcaataaaa
     1201 aacagacgcg ggaattgtaa tgaaacagat ccgttttgag tgctgtggtc tggtaattta
     1261 atgtagtcaa ccgacaggaa gagaagcgaa tttcttcatg aatatttacc acgacacctt
     1321 tgcccgccat ttgctgtccg caccggatgc gcccgccctt tgcgtgcagg gcagcacttt
     1381 ttcctaccgc gatctggagt gctgggtaaa cggctgtcgt attgcgttgc gtgattgtgg
     1441 cgtgcagacc ggcgatcctg tcgccgtgct ggccagccgc agtctggtgg cgtatgtggc
     1501 gattctggcc tgtgcggcgg agggtttcac ctgggtgccg ctgaatccgg catttcccga
     1561 tgcccgtaac cagcgcatgc tcacgctgtc cgcggcgcgg gtgctggtgg cggggcggga
     1621 agcggcagac cagagccagc gtctggcgga ggacgcgaat ttgcaggcag tgctggcacc
     1681 ggaggaggcc gtgccggacg cccgccggtt tgcgccatcg gatcaggtgt tgccgctggt
     1741 gcggtttgcc gccgatttgc cggcctacct gcttttcacc tcgggcacca ccggcgtgcc
     1801 caagggcatc ccggtcagcg tgggcaacct gctcgcctac ctgcagaatt tccacgccct
     1861 gtatcccctg ccggcgggcc tgcgctattc gcagatgttc gacctgacgt tcgatctgtc
     1921 ggtacacgat ctgttcgtgt gctggcagca gggcggctgt ctggtgattc ctaccgccca
     1981 ggatctgctg atgccgctgc agttcgtgcg ccggcagcag atccaggtct ggttttccgt
     2041 gccctccatg gccagcatcg ccaagcagag tgggctcttg cggccgggcg tattgccgtc
     2101 actgcagttc gcatttttct gcggcgaggc gttgcccacc gccagtgcac tggcctggat
     2161 ggatgcggcg cccaatgccc gcgtcgccaa tctgtacggt cctacggaag cgaccattgc
     2221 catcacggcg ttcgaactgc agcgcgaccg cgacgactgg cgccagttga gcgtggtgcc
     2281 catcggctgg ccctatcccg gccagcaggc ccgcgtggtg cgcgaggacg caacgctggc
     2341 ggcgccggga gaaagcggtg aactctggct ggcgggcacg cagctcagcg cgggttatct
     2401 ggcagacgcc gcaaaaaccg ctgccgtgtt cgtgcagcag cggttcgccg gatgtgatga
     2461 aacacgctgg taccgcaccg gcgattgcgc caccatccat ccggatttcg gtatcctcta
     2521 tgccggccgt ctcgaccgtc aggtgaagat caacggctac cgggtggaat tgcaggaagt
     2581 ggagcactgc ctgcgccagg tggcgggcac ccagctggcg gctgtcgtgc cctggccgcg
     2641 cagtgccgac ggcctgttta ccggcgtggt cggatttgtg gcacaggcct ggcagacgga
     2701 ggatcaggtg ctggcgcaat tgacggccac cctgccggcc tacatgcagc cccgtcagat
     2761 catcagtctg gagcgactgc cggtgaatgc aaatggcaag gtggattacg gcgcgctgca
     2821 ggcaatgctg gcacagtgaa gccaggcggc ggccgtcatg cgcgccggga tttggggtca
     2881 ggccaaaacg tcggcagcct gtgctggtct ggcccacccc atgccctccc ggacccaatc
     2941 gtgtacacta cttccctcac gccaaagccc ggtccagcca ccggcgcctg caaccaaaaa
     3001 aacctgaaag gattagtcca tgttgagatt cccggagatc gatccggtcg cgatttccat
     3061 cggccccctc gacatccgct ggtacgggct gatgtacctc gccggctttg tgtccggctt
     3121 catgctgttg aaccggcgtg cccgccggcc ccagtccggc tggaccacag agcaggtgag
     3181 tgacctgatt ttctacggtg cactgggcgt gatcctgggc ggccgtattg gctatgtgct
     3241 gttctacaat ttcggtgcgt tcctcaataa ccctctcacg ctgttccagg tctggaacgg
     3301 cggcatgtcg tttcacggcg gcctgttggg cgtgatgtgc gcagtgctgc tgttcgcgcg
     3361 taacatgcag aaaaactatt gggacgtgct ggatttcggc gcgcccatcg tgccgatcgg
     3421 actgggcctg ggccgcatcg gcaatttcat cggcggcgaa ctggtggggc gccccaccga
     3481 cggaccctgg ggcatggtgt tcccgcacgt ggacaatctg gcgcggcatc cgtcgcagct
     3541 gtatcaggcc acgctggaag ggctggcgct gttcagcatc ctctggttct tttcgcgcaa
     3601 gccgcggccc cgttacgcgg tgtcgggcct gtttgcgctg ttttacggcc tgttccgctt
     3661 cattgtggaa tttgcgcgcc agccagatgt gcaactgggc ttcgtcgcct ttgactggct
     3721 caccatgggt cagctgctgt cgctgccgct gatcgcggtg ggcctcacgc tgctttatct
     3781 ggcctacggg cgtcattctt catattcaaa ttccaagatc tgaggccgcg ttcgatccat
     3841 gaaacagtat ctcgatctac tgcgccacat caaagaaaac ggcgtcacca aaagtgatcg
     3901 caccggcacc ggcaccatca gtctgttcgg ctaccagatg cgcttcaatc tggcggacgg
     3961 ctttccgctg gtcaccacca agaaagtgca tctgaaatcg attattcacg aactgctgtg
     4021 gttcgtgcgc ggcgaaacca acatcgccta tctgaaagaa aacggtgttt cgatctggga
     4081 cgaatgggcc gacgccaacg gcgaactggg cccggtgtat ggcaagcagt ggcgcagctg
     4141 ggaaggtccc aacggtgaaa ccgtcgatca gctgcaacag ctggtggacg agatcaaacg
     4201 caatccggat tcgcgccgcc tggtgttgag cgcctggaat ccggtggtgc tgccggacgt
     4261 gaaattctcg ccgaaggaaa acgcggcggc gggcaagcag gcgttgccgc cgtgccactg
     4321 cctgttccag ttttatgtgg tggaaggcag gctgtcgtgc cagctgtatc aacgcagtgg
     4381 cgatgtgttt ctcggtgtgc cgttcaatat tgcgtcctat gccctgctca ccctgatggt
     4441 ggcgcaggtg tgcggcttga aacccggaga gttcgtgcac acgctcggcg acacgcacct
     4501 ctacctcaac cacctggagc aggcgcgcga gcagctcgca cgcctgccgc gcacgctgcc
     4561 gaagatccgg ctcaa
//
LOCUS       DASUEG010000249         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1904000, whole genome shotgun sequence.
ACCESSION   DASUEG010000249 DASUEG010000000
VERSION     DASUEG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904000"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFX11_04635"
     CDS             complement(<1..224)
                     /locus_tag="VFX11_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4937939.1"
                     /translation="MSWHYIEKGRGRPLILLHGIGMNAQAWSPVFDRLAIQRRVIALD
                     IPGFGKTPGLPDTVEPGPAAMVDGLRQTLHA"
     gene            433..876
                     /locus_tag="VFX11_04640"
     CDS             433..876
                     /locus_tag="VFX11_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX4937940.1"
                     /translation="MYTIALMLHVLGATVWTGGHLVLSFCILPVALRERSPTYIRTFE
                     QRFEKIGILALVIQIGTGLYMAFQLQPDFSQWFGFGNLVSQTLTLKLSFLAATALLAV
                     DARLRIIPNLTEHNLTSLAWHVIPVTVISVLFVLVGVSFRTGGFA"
     gene            873..2573
                     /locus_tag="VFX11_04645"
     CDS             873..2573
                     /locus_tag="VFX11_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HEX4937941.1"
                     /translation="MTILGIDLGTTNSACAIWRDGKPELIPNRFGDFLTPSVVGLDDN
                     GELLVGKFAKQRLLSHPHLCASVFKRYMGTDNQQTLGKKNFTSPELSALVLKSLKQDA
                     ETLLGETVTEAVISVPAYFNDVQRKATRLAGELAGLKVERLINEPTAAAMAYGLHEKP
                     EGSRFMVVDLGGGTFDVSIMEYFSGVLEVHASAGDNFLGGEDFLEALVQWYLELTGID
                     KNALNRIQVQQLYSTLEQAKRKLSSVEEVKLDLILPQQKEPISLRRDTFNQRVQSLLS
                     RIQLPVERALNDADISPTDLDDVVLVGGATRMQIFRSMIARMFRRMPAANLDPDLVVA
                     MGAGIQAGLKAKDAALDDIVLTDVCPYTLGTGIHNENDPGGKQGTLFLPIIERNSVVP
                     VSVVKTLYTVTDNQSRVLVDIYQGESRLTRNNVKLGELEVKVPKNKAGAESIDVRYSY
                     DMNGLLEVDIKVNSTGEVFNTLIKNNAGNLSETDIAKTREKLATLKFHPREDEVNRAL
                     LHRAERMFESALGEKREHLSHLLQHFESVLEGQNPHEIKLAQKRFHEMLDELEGEHWF
                     "
     gene            2573..3850
                     /locus_tag="VFX11_04650"
     CDS             2573..3850
                     /locus_tag="VFX11_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4937942.1"
                     /translation="MNYWDVLQLAPTTDIRAIKKAYAVLLKQNKPDENPEGFQRLHAA
                     YQQAQAWAERHAEEAPRRAPRTVAPAAGPIGTDAPPETPNETLPAETEQIAAVMPDSR
                     PPPAEPTSLIQRPVAEEPLPEHRWQDAPSRPDADVPLIRPEPTPVHGQAMHPHEEPLS
                     AEEEAWENFLDQQWDSLVDQAEAVLEQPALRNDPRAWDFLVHAEALLDIEFKAEFSLR
                     FLQRLVQIFFEQRESGELMLIPDTIRYLNNTFWWDQRRHHLEDHFEEDELDQFLAWWQ
                     PPPAPSSFSHAAKDAGPLIPYANYYRRWLALIIDGALLFACGWLINRFGHFMDESMVF
                     AALFMIAAYAILNPLFESSPLQATPGKLWCRMKVCDAAGNRIPLWRALLRCALFGVSL
                     YFLYITVIVNMLIWDGRLLHDRLSGSCVLRRQP"
BASE COUNT          821 a         1228 c         1184 g          757 t
ORIGIN      
        1 gcgtgcaggg tctggcgcag gccgtccacc atggcggcgg ggccgggctc caccgtgtcc
       61 ggcaggccgg gggttttgcc gaaaccgggg atgtcgaggg caatcacccg gcgctggatc
      121 gccagacggt caaataccgg cgaccaggcc tgcgcgttca tgccgatgcc gtgcagcagg
      181 atcagggggc ggccacggcc cttttcgatg taatgccagc tcatgggaat atccttcttg
      241 ttctggaaag cgtcttgttc tggaatatgg gtgtctgcga ctctaagccg gatgatttaa
      301 aacgcccgca gcttatcacg cccgccaaca cctgcccatc cacagcctga ttttcgaccg
      361 ccccggcaac tgcccattgg ttgtacccgt gcgccggtct atcatagccg gctcaaagaa
      421 ggagcctccc ggatgtacac gatagcgttg atgctgcatg tgttgggtgc cacagtctgg
      481 accggcggac atctggtgct gtccttctgc attctgcccg tggcgctccg tgagcgctca
      541 ccaacttata ttcgtacctt cgaacagcgg ttcgagaaga tcggcattct ggccctggtg
      601 atccagatcg gtaccggcct ttacatggcg ttccagctac agccggattt cagccagtgg
      661 ttcggcttcg gcaacctggt cagccagacc ctcaccctca aactcagttt cctggcggcc
      721 accgcactgc tggccgtcga cgcgcgcttg cgcatcattc ccaacctcac cgagcacaac
      781 ctgacatccc tggcctggca cgtcatcccg gtcaccgtga tttcggtgct gtttgtgctg
      841 gtgggcgtca gcttcagaac aggcggattt gcatgaccat tttgggtatc gacctgggta
      901 ccaccaacag cgcctgcgcc atctggcgcg acggcaagcc ggaactgatt ccgaaccggt
      961 tcggcgattt tcttacgccc tcggtggtgg ggctggacga caacggcgaa ctgctggtgg
     1021 gcaagttcgc caagcagcgc ctgctgagcc atccgcatct gtgcgcgtcg gtgttcaagc
     1081 gctacatggg caccgacaac cagcagaccc tgggcaagaa aaatttcacc tcgccggaat
     1141 tgtcggcgct ggtgctgaaa agcctgaagc aggatgcgga aaccctgctg ggtgaaaccg
     1201 tcaccgaagc cgtgatcagc gtgcccgcct atttcaacga cgtgcagcgc aaggcgacac
     1261 gactggcggg cgaactggcg ggtctgaaag tggaacgcct gatcaacgaa cccaccgccg
     1321 cagccatggc ctacggcctg cacgaaaaac ccgaaggcag ccgcttcatg gtggtggatc
     1381 tgggcggtgg tacgtttgat gtttccatca tggaatattt cagcggcgtg ctggaagtgc
     1441 atgcatcggc gggcgacaac tttctgggcg gcgaggattt tctggaagcg ctggtgcagt
     1501 ggtatctgga actcaccggc atcgacaaga atgcgctgaa ccgcatccag gtgcagcagc
     1561 tgtattccac gctggaacag gccaagcgca agctgtcgtc ggtggaggaa gtgaagctcg
     1621 acctgatcct gccgcaacag aaggaaccca tcagcctgcg ccgcgacacg ttcaaccagc
     1681 gtgtgcaatc gctgctgtcg cgcatccagc tgccggtgga acgcgcgctg aacgacgccg
     1741 acatttcgcc aacggatctg gacgacgtgg tgctggtggg cggtgcgacg cggatgcaga
     1801 ttttccgttc catgatcgcg cgcatgttcc gccgcatgcc ggccgcgaat ctcgatcccg
     1861 atctggtggt ggcgatgggc gccggcattc aggccggcct gaaagcgaaa gacgccgcgc
     1921 tggatgacat cgtgctgacc gacgtctgcc cctacaccct gggtactggc attcacaacg
     1981 aaaatgatcc cggcggcaag cagggcacgc tgttcctgcc gatcatcgag cgcaattccg
     2041 tggtgccagt gagcgtggtg aaaacgcttt acaccgttac cgacaaccag tcccgcgtgc
     2101 tggtggatat ttaccagggt gaatcccggc tgacgcgcaa caacgtcaag ctgggcgagc
     2161 tggaagtgaa agtgccgaaa aacaaggccg gtgccgaaag catcgacgtg cgttacagct
     2221 acgacatgaa cggcctgctg gaagtggaca tcaaggtgaa ttccaccggc gaagtgttca
     2281 acacgctgat caagaacaac gccggcaacc tgagcgaaac cgacattgcc aaaacgcggg
     2341 agaaacttgc cacactgaaa ttccacccgc gcgaggacga agtgaaccgc gcgctgttac
     2401 accgcgccga gcgcatgttc gaatcggccc tgggcgaaaa gcgcgaacac ctgtcccacc
     2461 tgttgcagca tttcgaatcc gtgctggaag gacagaaccc gcacgagatc aaactggcgc
     2521 agaaacgttt ccacgaaatg ctggatgaac tggaaggcga gcactggttc tgatgaacta
     2581 ctgggacgtg ttgcagctgg cgcctaccac cgatattcgc gccatcaaga aagcgtacgc
     2641 cgtgctgctc aagcagaaca agcctgatga gaatccggaa ggcttccagc ggctgcatgc
     2701 tgcataccag caggcacagg cctgggcgga acggcatgcg gaggaagcgc cgcgcagggc
     2761 accacgcacg gtcgcgccgg cagccggacc catcggcaca gatgcaccgc ctgaaacacc
     2821 aaacgaaacg ctgcctgcgg aaacggagca gatcgcggcc gtcatgccgg attcccgccc
     2881 tccgccggca gagccaacat cgctgatcca gcgtcctgtg gcggaagaac cattgccgga
     2941 acatcggtgg caggacgctc cgtcacgtcc cgacgcggac gtgccactga tccgccccga
     3001 acccactccc gtgcacggcc aggccatgca tccacacgaa gaacccctgt cggcggagga
     3061 ggaagcctgg gaaaacttcc tcgaccagca gtgggacagt cttgtggatc aggcggaagc
     3121 agtgctggag cagcccgcgt tgcgcaacga tccgcgcgcc tgggattttc tggtgcacgc
     3181 cgaagcgctg ctggacatcg aattcaaggc ggaattttcc ctgcgttttc tgcaacgtct
     3241 ggtgcagatt tttttcgaac agcgggaaag cggcgaactg atgctgatac cggacaccat
     3301 ccgctacctg aacaacacct tctggtggga tcagcgtcgc catcatctgg aagatcattt
     3361 cgaagaagac gaactggacc agttcctggc ctggtggcaa ccaccgccag caccctcttc
     3421 tttctcacat gcggcgaagg atgccggtcc gctgattccc tacgccaact attatcggcg
     3481 ctggctggcg ctgatcattg acggcgcgct gctgttcgcg tgcggctggc tgatcaaccg
     3541 gttcggccac ttcatggatg agtccatggt gtttgccgcc ctgttcatga tcgccgccta
     3601 tgccattctc aatccgctat tcgagtcctc gccgctgcag gccacgcccg gcaaactctg
     3661 gtgcaggatg aaagtgtgcg acgccgcagg caaccgcatc cctctgtggc gggcattgct
     3721 gcgctgcgcg ctgttcggcg tcagcctgta tttcctctac atcaccgtga tcgtcaacat
     3781 gctgatctgg gacggacgcc tgctgcatga ccggctgagc ggttcctgcg tgctccgacg
     3841 ccagccctga agggcagttg ttcccacatc atctaacccg acccgaaaac ccaactgctg
     3901 gctattggac gtgcagctgc ccctctacta gattcatgct gtcgtgccag cgcccgaggc
     3961 gaagttcatg gatcccatca gcagttccgt
//
LOCUS       DASUEG010000250         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1188760, whole genome shotgun sequence.
ACCESSION   DASUEG010000250 DASUEG010000000
VERSION     DASUEG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188760"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFX11_04655"
     CDS             complement(<1..453)
                     /locus_tag="VFX11_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4937943.1"
                     /translation="MLGRITRPLLACCLLLLASAAVASHPASAQATSRPDFRFLESTG
                     ALTPEQALQALKQAPAQVVSDEGIDIRNRHATYWLLADLRVDSDEARVDRILRVKHLY
                     PKFDRLELFLFADGQLVDHQINGESVPYSQRPTPGLQMAFALRLESGKP"
     gene            complement(619..1641)
                     /locus_tag="VFX11_04660"
     CDS             complement(619..1641)
                     /locus_tag="VFX11_04660"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HEX4937944.1"
                     /translation="MATYDKLIDASGQPHYGRFDAPVQQVNHTDFHYLNAMDRPAGQL
                     ARHFHYKQFQFIGVTGPRYTLGCAIVSLKYVSNAFVYLFDRLTGERREHSLLQPLSLG
                     CSMVNTPDAGMHRFSKGDFEFNFYAASEPRQRQVEIFGDARINIDLQISEPAGFQPLA
                     VCTRTGFTGWTYTQKAAALDAGGRIRWGDLDIPVDKEFLGSYDWSCGYLRRHTAWNWA
                     SLSGLLADGRRVGANLANGVNETGFTENAVWIDGQLHPLGPVMFEFDRNDRRKPWRVR
                     TADGRLDLAFTPAGERREKLDLLLLASNFTQLTGCFTGTFIADGGERIRLETTPGFCE
                     DHYAKW"
     gene            1681..2058
                     /locus_tag="VFX11_04665"
     CDS             1681..2058
                     /locus_tag="VFX11_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX4937945.1"
                     /translation="MALKHSHMLFVVISGLFFLVRGCWMLMDSPMLQKVWVKVLPHVN
                     DTLLLVCGIGLAVVTQQYPFVQAWLTVKVLLLVAYIVLGTVALKRGKTKTIRVVAFFA
                     ALACFLFMLSVARTHDPLGFLSY"
BASE COUNT          406 a          564 c          744 g          510 t
ORIGIN      
        1 gggtttgccg ctttccagcc gtagcgcgaa tgccatctgt aatcccggag tggggcgctg
       61 gctgtagggc acgctttcgc cgttgatctg atggtccacc aactggccgt ccgcaaacag
      121 gaacaattcg agccggtcga atttggggta caggtgcttt acacgcagga tgcggtccac
      181 ccgggcttcg tcgctgtcca cccgcaggtc tgccagcagc cagtaggtgg cgtggcggtt
      241 gcggatgtcg atgccttcgt cgctgacaac ttgggccgga gcctgtttca gggcctgaag
      301 ggcctgctct ggtgtcagcg cgccggtgct ttccagaaaa cggaaatccg gccgggaggt
      361 ggcctgtgca gacgcagggt gactggcgac tgcggcgctg gccaacagca acaggcagca
      421 ggccagcagt gggcgcgtga ttcgtccgag caacaggttc cagttgtgtg ttccaatacc
      481 catctagtgt gtgagctaat tcaaatcagg tgggtggggc cataaaaaac gggcgttcgc
      541 aaaacagctg gtgcccgttg ttatggttat cgttctagaa gtgtagctga tcaaaaaacg
      601 tccgcgatta ggtgcttatc accatttggc ataatggtct tcgcagaacc cgggagtcgt
      661 ttccaagcga atgcgctcac cgccgtctgc tataaatgtc cctgtaaaac aaccggttaa
      721 ttgggtgaaa ttagaggcca gcaggagcaa atcgagcttt tcgcggcgct ctccggcggg
      781 cgtgaaggcg aggtcgagcc ggccgtcggc ggtgcggacc cgccagggtt tgcggcggtc
      841 gttgcggtcg aattcgaaca tcacagggcc gagggggtgc agctggccgt caatccagac
      901 ggcgttttcg gtgaagccgg tttcgttgac gccattggcc aggttggcgc ccactcgccg
      961 gccgtctgcc agcaggccgg acaggctggc ccagttccag gcggtgtgac ggcgcaggta
     1021 gccgcaactc cagtcgtagc tgccaagaaa ttctttgtcc accggaatgt ccaggtcgcc
     1081 ccagcggatg cgcccaccgg catcgagtgc ggcggctttc tgggtatagg tccagccggt
     1141 gaagccggtg cgggtgcaga cagcaagtgg ctggaagccg gccggctcgc tgatctgcag
     1201 gtcgatgttg atgcgggcat cgccaaagat ttccacctgg cgctggcgtg gttcggacgc
     1261 tgcgtagaaa ttgaattcga agtcgccctt gctgaaccgg tgcatgcctg catcgggggt
     1321 gttgaccatc gagcagccca gactgagggg ttgcagcagg ctgtgttccc gccgctcgcc
     1381 ggtcagccgg tcaaacagat agacgaaagc gttgctgaca tatttgaggc tgacgatggc
     1441 gcagcccagg gtgtagcggg ggccggtgac gccaatgaac tggaactgtt tgtagtggaa
     1501 gtgccgggcc agctggccgg cggggcggtc catggcgttc aggtagtgga aatcggtgtg
     1561 gttcacttgc tgcacggggg cgtcgaaacg gccgtaatgg ggttgcccgc tggcatcaat
     1621 caacttatcg taggtggcca ttttttcatt catacgattg tgggggagat ttttatgtat
     1681 atggcgctca agcatagcca tatgttgttc gttgtcatca gcggactgtt ttttctggtg
     1741 cgtggctgct ggatgctgat ggactcgcca atgctgcaaa aagtgtgggt gaaggtgctg
     1801 ccgcacgtca atgatacgct gctgctggtg tgtggtatcg gtctggcggt ggtgacacag
     1861 cagtatccgt tcgtgcaggc ctggctgacg gtaaaggtgc tgctgctggt ggcttacatc
     1921 gtgctgggca cggtggcgct gaagcggggc aagaccaaga cgatccgtgt ggtggccttt
     1981 tttgctgcgc tggcctgttt tctgttcatg ttgtccgtgg cgcgtaccca tgatccgctg
     2041 ggatttttga gttactgact ggcgggtagt aaaaggcgct gattgcgctc gggagatcga
     2101 aattctgcct tgcgcaatcg atacaaactc catgactgtt gattcatgga atgattcagc
     2161 tgtttgggtg ttgatctggt tgtctcggtg ccacgcgagt agtcccatac caatgcgtgg
     2221 ctca
//
LOCUS       DASUEG010000251         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1528913, whole genome shotgun sequence.
ACCESSION   DASUEG010000251 DASUEG010000000
VERSION     DASUEG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528913"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFX11_04670"
     CDS             complement(<1..1082)
                     /locus_tag="VFX11_04670"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4937946.1"
                     /translation="MKHHQLDVLIVGAGLSGIGAACHLARKCPDYRYGIVERRTRIGG
                     TWDLFRYPGIRSDSDMFTLGYSFRPWTDTKMLADGPAIRQYIEDTAREYQVKKHIHFG
                     RKVVSESWDSTKNLWTVLVVNEETGEEETFTARFLFNCTGYYNYDAGYTPRIPGIKRF
                     GGEVIHPQHWPENYDYTGKRVVVIGSGATAVTLVPAMTDKAAHVTMLQRSPTYVVTVP
                     EQDLISKSLRLLLPEMAVYRMARARNILLQRTVFKLSIKKPKAIRRLLRVATRKQLGP
                     EFDMRHFTPHYNPWEERMCAVPKGDLFKALREKKASVVTDHIETVTKTGVKLKSGNLL
                     PADVIITATGLDLQMFGGAKIFVDGE"
     gene            complement(1102..2025)
                     /locus_tag="VFX11_04675"
     CDS             complement(1102..2025)
                     /locus_tag="VFX11_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4937947.1"
                     /translation="MKDIAQGRIRQAPLSEFTQAPLEIASPDGVVLRGARFGQPDSPV
                     VVLLHGGGQTRHAWTDTAHLLAASGFCAVSLDARGHGDSDWCPRGDYSADALVRDLRA
                     ILGSFSSPPYLVGASMGGLTALLTLGEDHTLDCRGLVLVDVAARLESDGVRRIIDFMR
                     QHTAGFESLEQARDAVNAYNPTRKASASPAGLLKNLRHAPDGRLFWHWDPAFLGDVSM
                     EGSGKGMFDPARLDRAAARLTLPVLLIRGFHSDVLSEEGARDLLARIPQARYQVVEQA
                     GHMVVGDRNNAFTHAVLEFLNHAGDTSSANT"
BASE COUNT          486 a          684 c          660 g          481 t
ORIGIN      
        1 tcttcgccgt caacgaatat ctttgcaccg ccaaacattt gcaggtcaag cccggtagcg
       61 gtaatgatca cgtcggcagg caacagattt cctgacttca gttttactcc ggttttggtc
      121 actgtctcga tatgatcggt aacaaccgag gctttctttt ctcgaagcgc cttgaacaga
      181 tcgccttttg gcaccgcaca cattctttct tcccagggat tgtaatgcgg ggtgaagtgc
      241 cgcatgtcaa actccgggcc gagttgctta cgggtggcga cgcgcagcaa gcggcggatg
      301 gctttcggtt ttttgatcga cagtttgaaa acggttcgtt ggagcaatat gtttctcgct
      361 cgcgccattc tgtagaccgc catttccggc agtaagaggc gcaggctttt cgagatcaga
      421 tcctgttccg gaacggtgac aacataagtc ggtgagcgtt gcagcatggt aacgtgggcg
      481 gctttatcgg tcatggccgg gaccagagtg accgcagtcg ccccgctacc aatgacgacg
      541 acgcgcttgc ctgtgtaatc gtagttttcc ggccagtgct gtggatggat cacttctccg
      601 ccaaagcgct tgatgccggg tattcgtggg gtgtaaccgg catcgtagtt gtagtacccg
      661 gtgcagttga acaggaacct ggcggtgaac gtttcttcct cgccggtttc ctcattgacg
      721 accagtaccg tccacaggtt cttcgtactg tcccagcttt cagacaccac cttgcggccg
      781 aaatgaatat gcttttttac ctggtattca cgagcggtat cctcgatgta ttggcgtatg
      841 gcaggcccgt cggcaagcat tttggtatca gtccacgggc ggaagctata gcccagcgtg
      901 aacatgtccg agtccgagcg aattcccggg taacgaaaca gatcccaggt gccaccgatg
      961 cgggtgcggc gctcgacaat accgtaacgg taatcaggac atttgcgtgc cagatgacag
     1021 gctgcaccaa tgccggacag accagccccg acgattagca catccaactg atgatgtttc
     1081 atggtaaggc tccggaaatt attaagtgtt cgcggacgat gtatcgccag catggttaag
     1141 aaattccagc actgcatggg tgaacgcgtt attgcgatcg ccaacaacca tatggccggc
     1201 ttgctctacc acctggtagc gagcttgagg aatgcgcgcc aacagatccc gggcgccctc
     1261 ttcgctaagc acatcactgt gaaatccgcg aatcagcagc acgggtaacg tcagtctggc
     1321 ggcagcccgg tcgagtcggg cagggtcaaa catccctttg ccgctgcctt ccattgaaac
     1381 gtcgccgaga aaggcgggat cccagtgcca gaacagcctg ccgtcaggag cgtggcgcag
     1441 atttttcagc agaccggcag ggctcgctga ggccttgcgc gtcgggttat aggcattcac
     1501 cgcgtctcgc gcctgctcca atgactcgaa gcctgccgtg tgttgccgca tgaaatcgat
     1561 aattctgcgt acgccatcgc tttccaggcg cgcggccacg tccaccagca ccagaccacg
     1621 acagtccagc gtgtgatcct cccctagggt gagaagggcg gtgagcccgc ccatcgaggc
     1681 accaaccaga tacggtggcg atgaaaatga gcccagtatt gcccgcaggt cgcgcaccag
     1741 ggcatcggcg ctgtagtctc cccgcggaca ccagtcgctg tcgccatgcc cacgggcatc
     1801 caggctgaca gcgcagaacc ccgaggcggc cagcagatgc gcggtatcgg tccaggcatg
     1861 acgggtttgt ccaccaccgt ggagcagaac gaccacaggc gaatcgggct gaccaaagcg
     1921 ggcgccccgc aagaccacgc cgtccggaga agcgatctcg agcggcgcct gtgtgaattc
     1981 actcaatggc gcctgcctga tccgcccctg tgctatgtcc ttcattcagt aaacagttcc
     2041 attaagttgg ttccccaacc aaaatagagg agcccggcat aaaaggcaag cagttagggt
     2101 cacttagtct cattgcatgc atgggtgcgc gcgagctagg cggggcctgc tccatcaggt
     2161 ggtaatcctt gcctgccgca aggccgccgc ccagcccgcg ggccaggcgc ccgccgtcag
     2221 cgagtccgcc gagctgcgcc gcctcaaggc cgaactcaag cgcgtcaccg aggaacgcga
     2281 catcctaaaa aaagccgccg cgtactttgc c
//
LOCUS       DASUEG010000252         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_239619, whole genome shotgun sequence.
ACCESSION   DASUEG010000252 DASUEG010000000
VERSION     DASUEG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239619"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFX11_04680"
     CDS             <1..405
                     /locus_tag="VFX11_04680"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4937948.1"
                     /translation="DIPEPWYLLAIPACHAPTAELFQEKQLTRNSDVITICAFLAGAG
                     HNDFEPVLRKRFPLIDRCLHLMDTVSKARVTGSGACLFSSFESEQLAIAARQQLATQM
                     PDFSLSDQDVKWQVTRGMNRSPLFTGPLAGNG"
     gene            491..565
                     /locus_tag="VFX11_04685"
     tRNA            491..565
                     /locus_tag="VFX11_04685"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Gln,seq:ttg)
     gene            610..1563
                     /locus_tag="VFX11_04690"
     CDS             610..1563
                     /locus_tag="VFX11_04690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX4937949.1"
                     /translation="MSNLIIFTGNATPELATKVCTHLHIPLGKADVGKFSDGEIAVEI
                     NENVRGSDVFIIQSTCAPTNDSLMELIVMADALRRASAGRITAVVPYFGYSRQDRRVR
                     SARVPITAKVVADMITTVGIDRVLTVDLHADQIQGFFDIPVDNVYGSPILLEDILKQN
                     YQNLMVVSPDVGGVVRARAVAKQLDDCDLAIIDKRRPKANQSQVMHIIGEVEGRTCIL
                     VDDMVDTAGTLCKAAEALKDHGATKVVAYCTHAVLSGGAIDNLTKSKLDELVVTDTIP
                     LSAASRACGRIRQLSLAPMLAECVRRISNEESLSAMFAPKD"
     gene            1635..2267
                     /locus_tag="VFX11_04695"
     CDS             1635..2267
                     /locus_tag="VFX11_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX4937950.1"
                     /translation="MSNEFTIEATTRSDLGKGASRRLRRLENRVPGILYGADKAPTPI
                     SVLGKDLSKALENEAFYSHILSLKLDGGDVQVVLKDLQRHPAKNNPTHVDFQRIDATH
                     KLHMHVPLHFLNEDKCVGVKTGGGIVSHQLTEVEVVCLPKDLPEFIAVDVADLDMGKS
                     IHLSQLTLPAGVELRALQLGEDHDLSVVSVIATRASIGEEEAKPAAPAAE"
     gene            2291..2881
                     /gene="pth"
                     /locus_tag="VFX11_04700"
     CDS             2291..2881
                     /gene="pth"
                     /locus_tag="VFX11_04700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX4937951.1"
                     /translation="MKEGIDLIVGLGNPGRQYEETRHNAGAWFVERLARRFSVSLSPD
                     SKYQALTGRAVIHGRDIRLLIPATFMNLSGQSVVPFASFYKIPPQRMLVAHDELDLPP
                     GTAKLKQGGGHGGHNGLRDIISKLGNNADFGRLRIGIGHPGHASQVSSYVLGKAPAKE
                     QQLIDDAISEAISILPDLLDGNASKAMNRLHAFAAT"
     gene            2921..4012
                     /gene="ychF"
                     /locus_tag="VFX11_04705"
     CDS             2921..4012
                     /gene="ychF"
                     /locus_tag="VFX11_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX4937952.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTKAGIEAQNFPFCTIEPNTGVV
                     PVPDPRQDALAAIVKPERVVPTTVDFVDIAGLVAGASKGEGLGNQFLGNIRECDAIAH
                     VVRCFEDDNIIHVAGKVNPLSDIETINTELALADLDAVDKAMLRAAKLAKGGDKEAAA
                     RMKALEKLQPILNEGKPIRSVALTDEESKAIRTLNLLTLKPTMYIANVMEDGFENNPL
                     LDAVRKLAEAENAQVVPICAKIESEIAELDEADKADFLAELGMKEPGLNRVIRAGYAL
                     LGLQTYFTAGVKEVRAWTVKVGATAPQAAGVIHTDFEKGFIRAEVIAYDDFIQYKGEA
                     GAKEAGKWRLEGKEYIVKDGDVVHFRFNV"
     gene            4093..4169
                     /locus_tag="VFX11_04710"
     tRNA            4093..4169
                     /locus_tag="VFX11_04710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4127..4129,aa:Met,seq:cat)
     gene            complement(4241..4483)
                     /locus_tag="VFX11_04715"
     CDS             complement(4241..4483)
                     /locus_tag="VFX11_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937953.1"
                     /translation="MKRPKATTSISERYSLDRLEAMVQRDIVRLEEQLARVEGDTNNQ
                     TRVSTARTYREMIADRRELLAQIRRQSSEFLGQAVV"
BASE COUNT         1050 a         1490 c         1267 g          853 t
ORIGIN      
        1 gacattcccg aaccctggta cctgctcgca atccctgcct gccatgcacc gacagctgaa
       61 cttttccagg aaaaacaatt gacaagaaat tccgacgtta tcacaatatg cgccttcttg
      121 gccggggccg gacataacga cttcgaaccc gtgctgagaa aacgctttcc actgatcgat
      181 cgatgcctgc atctgatgga cacagtgagc aaggcaaggg tgaccggaag cggtgcctgc
      241 ctgttcagtt ctttcgaatc cgaacagctc gccatcgcgg cacgccagca actggcaacg
      301 caaatgcccg acttttcatt gagcgatcaa gacgttaaat ggcaagtcac ccgcggcatg
      361 aaccgatccc ccctgtttac tggaccgctc gccggcaacg gctagaatcc agaaaaaacc
      421 tctaatacat ttggcgattt tctggtagta ttccgcgccc agactgatcg ccagatcgtc
      481 aaagcacaat tgggccgtcg ccaagcggta aggcagcagg ttttgatcct gccataccta
      541 ggttcgaatc ctagcggccc agccattttt tctgatcgat ccccactgac agattcaagg
      601 tgacccatcg tgtccaatct gatcatattc accggcaacg ccacgcccga gctggcgacc
      661 aaggtatgca cccatctgca tattcccctg ggcaaggccg atgtagggaa gttcagcgac
      721 ggcgagatcg ccgttgaaat caacgaaaac gtgcgcggca gcgatgtctt tatcatccag
      781 tccacctgcg cacccaccaa cgacagcctg atggagctga tcgtcatggc tgacgccctg
      841 cgccgcgcct cggccggccg catcaccgcc gtggtcccct acttcggcta ctcccgccag
      901 gaccgccgtg tacgttccgc ccgggtcccc atcaccgcca aagtggtggc agacatgatc
      961 accaccgtcg gcatcgaccg cgttctcacc gtcgacctgc acgccgacca gatccagggc
     1021 ttcttcgata tccccgtgga caacgtctac ggctcgccga tcctgctgga agacatcctg
     1081 aaacagaatt atcagaacct gatggtggta tcgcctgacg tcggcggcgt ggttcgcgcc
     1141 cgcgctgtag ccaaacaatt ggacgactgc gatctggcca tcatcgacaa acgccgcccg
     1201 aaggccaacc agtcgcaggt catgcacatc atcggcgaag tcgaaggccg cacctgcatc
     1261 ctggtggacg acatggtcga caccgccggc accctgtgca aagcggccga agcactgaaa
     1321 gaccacggcg ccaccaaagt agtggcctac tgtacccatg ccgtgctgtc cggtggcgcc
     1381 atcgacaacc tgaccaaatc caaactggac gagctggtgg tgaccgacac catcccgctg
     1441 tccgcagcca gcagagcctg cggccgcatc cgccagctga gtctggcccc catgctggcc
     1501 gaatgcgtgc gccgcatcag caacgaagaa tcactcagcg cgatgttcgc tcccaaggac
     1561 tgaacagcgc gcttaaacct gccgccggac gctggtcgca agcgtccggt ggtttaacct
     1621 gaaggagcat gctcatgtcc aatgagttca ccattgaagc aacaacccga agcgatctgg
     1681 ggaaaggtgc gagccgccgc ctgcgtcgcc tcgaaaaccg cgttcccggc atcctgtacg
     1741 gcgccgacaa ggccccgacc ccgatttccg ttctgggcaa ggatctgtcc aaggccctgg
     1801 aaaacgaagc cttttactcc cacatcctgt ccctgaaact ggatggtggc gacgttcagg
     1861 tggtactgaa ggacctgcag cgtcaccccg ccaagaacaa cccgacccac gtcgacttcc
     1921 agcgcattga cgccacccac aagctgcaca tgcacgtgcc gctgcacttc ctgaatgaag
     1981 acaagtgcgt gggcgtgaag accggcggcg gcatcgtgtc gcaccagctg accgaagtgg
     2041 aagtcgtgtg tctgccgaag gatctgccgg agttcatcgc agtggacgtg gccgacctgg
     2101 atatgggcaa atcgattcac ctgagccagc tgaccctgcc cgccggcgtt gaactgcgcg
     2161 cactgcagct gggcgaagat cacgatctgt ccgtggtcag cgtcatcgcc acccgcgcca
     2221 gcatcggtga agaagaagcc aaacctgcag ctccggctgc cgagtaacac ctccagacag
     2281 ggcgccgacg ttgaaagaag gcatcgatct tattgtgggc ctgggcaatc cgggccggca
     2341 gtacgaagag acccgacaca acgccggcgc ctggtttgtg gaacggctgg cgcggcgatt
     2401 cagcgtcagc ctctctcccg attccaaata ccaggcactc accggccgcg ccgtcattca
     2461 cggccgcgac atccgcctgc tgatccccgc gactttcatg aacctcagcg gccagtccgt
     2521 tgtaccgttc gccagcttct acaagattcc accccagcgc atgctggtcg cccatgacga
     2581 actcgatctg ccccccggca cagccaagct gaaacagggc ggcggccacg gcggccacaa
     2641 cgggctgcgc gacatcatca gcaaactggg caacaatgcg gattttggcc gcctgcgcat
     2701 cggcatcggc cacccgggcc acgccagcca ggtctccagc tatgtgctgg gcaaggcgcc
     2761 cgccaaggaa cagcagctga tcgacgacgc catcagcgag gccatcagca tcctgccaga
     2821 cctgctcgac ggcaatgcca gcaaggccat gaaccggctg cacgccttcg cagccacctg
     2881 acactcaatt attcgaattc acttacaggg tacgaacacc atgggaatca aatgcggaat
     2941 tgtgggactg cccaacgtcg gcaaatccac cctgttcaac gcgctgacca aggcgggcat
     3001 cgaagcccag aacttccctt tctgcaccat tgaacccaac accggcgtcg tacccgtgcc
     3061 cgatccccgc caggacgctc tggctgccat cgtcaagccc gagcgcgtgg tccccaccac
     3121 cgtggatttc gttgatatcg ccggcctggt ggcaggcgcc tccaagggcg agggtctggg
     3181 caaccagttc ctgggcaata tccgtgaatg cgatgccatt gcccacgtgg tgcgctgctt
     3241 cgaggacgac aacatcattc acgtggccgg caaggtgaat cccctgtccg acatcgaaac
     3301 catcaacacc gaactggctc tggccgatct ggatgccgtg gacaaagcca tgctgagagc
     3361 ggccaagctg gccaagggtg gcgacaagga agcggcggcg cgaatgaagg cgctggagaa
     3421 actgcagccc atcctgaacg aaggcaagcc gatccgctcc gttgccctta cggacgaaga
     3481 gagcaaggcc atccgcaccc tgaacctgct gaccctgaag cccaccatgt acatcgccaa
     3541 tgtgatggaa gacggcttcg agaacaaccc gctgctggac gccgtgcgca aactggcaga
     3601 agcggaaaac gcccaggtgg tgcccatctg cgccaagatt gaatccgaga tcgccgaact
     3661 ggacgaggcc gacaaggctg attttctggc ggaactgggc atgaaagagc ctggactgaa
     3721 ccgggtaatc cgtgccggct acgcgctgct gggactacag acctatttca ccgctggcgt
     3781 aaaggaagtc cgtgcatgga ccgtaaaagt cggcgccacc gcgccccagg ccgccggcgt
     3841 gatccacacc gactttgaaa aaggctttat ccgcgccgaa gttatcgcct atgacgactt
     3901 cattcagtac aagggcgaag ccggtgcaaa ggaagccggg aaatggcgcc tggaaggcaa
     3961 ggaatacatc gtcaaggatg gtgacgtggt gcacttccgc ttcaacgtct gacccggatg
     4021 ggacggcgat ggaacagccg ttttttcttg acattaaggg gcggggttgc gagaatctgc
     4081 gccctcgaat ttggctatgt agctcagttg gttagagcac atcactcata atgatggggt
     4141 cggcagttca actctgccca tagccaccat aagcacaaaa aagcccggca ttgccgggct
     4201 ttttcgtttc ggatccctgt cgggtcatgt cacaacccgc tcaaacaacc gcctgcccca
     4261 gaaactcact ggattgacgg cggatctgcg ccagcaattc ccggcggtcg gcaatcattt
     4321 cccggtaagt acgggctgtg gagacacggg tctggttgtt agtgtcgccc tcaacccgcg
     4381 ccagttgctc ttccaggcgc acgatatcac gctgcaccat cgcctccagc cggtccagac
     4441 tgtagcgctc gctgatggaa gtagtcgcct ttggtctttt catcaaaagt tcgtccctaa
     4501 tgcctttagc cccccgaatc cccctcgcag gaccaaatcg tgccttggaa tattgacact
     4561 atttatggat ttaaaaaaac tagcctgcaa gtggttaatt tgcaagcgat ccaattttag
     4621 cgacgaacgg caaagcagca ctacttccgt tttgtataga
//
LOCUS       DASUEG010000253         7864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2032705, whole genome shotgun sequence.
ACCESSION   DASUEG010000253 DASUEG010000000
VERSION     DASUEG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032705"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1138)
                     /locus_tag="VFX11_04720"
     CDS             complement(11..1138)
                     /locus_tag="VFX11_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4937954.1"
                     /translation="MNFNLSEEQSLLADSVTRFIGNDYGYDIRKKHVALPDGFDRATW
                     ATFAELGWLCVPFSEEDGGIGGGPIETMIMMEEFGKGLVVEPYVSCVVLAGSVLKRAG
                     NASQKAELIGGIIDGSRLLTLAYLEPQSRFDLFDVKTTAKKDGDRYIVNGAKAMVLHG
                     GTADTFIVSVRTSGNPRDASGVSLLVVPADAAGVSVRDYPTLDGQRAAEVTFTNVSVP
                     AAALLGEEGKALAVLEAVADEARLALCAEAVGMMEKLYRETVEYTKTRKQFGVPIAVF
                     QALQHRMVDMFTLHAECKSLLLRATLTSATGDAEASRNIAALKYAIATKGQKVAHEAV
                     QIFGGMGMTDEMSVGMYLKRINVINTLFGNGDYHLQRFMAA"
     gene            complement(1149..2318)
                     /locus_tag="VFX11_04725"
     CDS             complement(1149..2318)
                     /locus_tag="VFX11_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4937955.1"
                     /translation="MDIQFKPEDLAFRDQVRAFLKSKLTPAIKDAVVGGHINKELIIQ
                     WQKILYEQGWIAPNWPKKYGGTDWDITKKYIFENECAEAGAPAVVPFGLKMVGPVIYK
                     FGNDAQKARFLPDILQSNVWWCQGYSEPGAGSDLAALQTKAVRDGDHYIVNGQKIWTT
                     YAQLADWIFCLVRTDPSAKKQEGISFLLIDMKTPGITVRKIDSIDNQHSLNEVFFDNV
                     KVPVENRIGEENQGWTYAKYLLTHERTAIAGVAQSKKAVQRLREIAALEMSGGRPLAE
                     DNDFRAKLAKIEIDLLALEYTELRAIASVAAGGAPGPESSILKIRGTEIQQALSELAV
                     EVCGHYAHALNVDAPVGNDYAWALSAKYLYGRAATIYGGSNEVQKNIIAKAVLGL"
     gene            complement(2554..2630)
                     /locus_tag="VFX11_04730"
     tRNA            complement(2554..2630)
                     /locus_tag="VFX11_04730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2594..2596),aa:Arg,seq:cct)
     gene            2756..3226
                     /locus_tag="VFX11_04735"
     CDS             2756..3226
                     /locus_tag="VFX11_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX4937956.1"
                     /translation="MPSHASIVLFCSLLCAAVLHPAAARATDSPSYGNVTVTEVTSIY
                     DADTFKVDIRQWPAVVGSRIAVRVKGIDAPEMQGKCEREKELARKAKQTTVAMLRAGK
                     KIELRNLQRDKYFRLLADVHVDGKNLATILLQAGLAYPYNGGTKKSWCQVSQPR"
     gene            3460..5409
                     /gene="nosZ"
                     /locus_tag="VFX11_04740"
     CDS             3460..5409
                     /gene="nosZ"
                     /locus_tag="VFX11_04740"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HEX4937957.1"
                     /translation="MKKDQSPNHNLDTDAPLDAGRRKFLTQTTAVAGLAGAGLTLGLA
                     GCKDESAPAAGAPAAKTEPHAAPAGHIKPGELDTHYGLWSGGHTGDLRVLGFPSGREI
                     HRIPCFTPDALVGWGITNESKAIMGTKADGTLKYTVGDSHHTHASYKDGNYDGRYAWI
                     NDKINARIARIRLDVFACDKITEIPHVQGFHGIFPDKRDPVDPAINYTTRVFCGAEFH
                     IPLVNDGKNMGDDSQYRALFSCVDAETMEVRWQVLIDGNCDLVATSFDGKLAASNQYN
                     TENGQRFENMMSAERDACIFFNIARIEQAVKDGKFTRIGDSKIPLVDGTRAANKNPAT
                     ALTAYVSVPKNPHGVNASPDGKYFICAGKLSPTATVIELAKVLDWFDGKLPELDKSIV
                     AEVELGLGPLHTAFDGRGNAYTTLFLDSQVVKWNVEAAIRFHGGDNTAKYVVDRLDVQ
                     YQPGHINASQSETMFADGKWLAVGCKFSKDRYLPVGPLHPENEQIVDISGDKMVLIAD
                     HPVRPEPHDFIIFKRDLVRTKQVYNMADFPNAVKSSSESGVFREGNKVTVKMTSQAPI
                     FSLRDFTLKLGDEVTLILTNLDNIEDLTHGFAIEKYNINFIVNPQETASVTFKADKPG
                     VFWCYCSHFCHALHLEMRSRMMVEA"
     gene            5517..>7864
                     /locus_tag="VFX11_04745"
     CDS             5517..>7864
                     /locus_tag="VFX11_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX4937958.1"
                     /translation="MNTLQALPKLLLLLSLLWTTQMALAGAYEAKLSEEIFSAPDLCA
                     HAPCTDVIPGATAFSQRKGQPPYVDAFADVNGKRELIGYVMLSTDITDTPAYSGKPVI
                     TLIGMDTTGKFTGAKVLKHSEPILLLGIPETALTNFLNQYVGLFAGDSIEVGHTRPDQ
                     DSIGLDGISGATVTVIAQNQVMLASATAVARQTGIIEPTIRNQATFAAAGKPQDWNSM
                     VAEGSVQQLRVEPEQVGLPASATPFIELWFGYLNQPDIGSAILGDNGYRNLMLQLKDG
                     EHALFVIRTDGGESFKGSGFVRGGIYDRVQVRQDTDSFTFRDRDYLNLYSIDVAGAPA
                     YTESAIYIIRSPAFSGAYPWKFVFLGNLVDKATGTRTFTNFDAEYWLPARYLQGGRPD
                     VVRPDPTWLKIWKNRLPEIILFTLFLIAVGVVYSQRNRLTRRSNRHNKWPVNGFKYVA
                     WVISVTLVGFGLLAQPSVTQILTWFHSLLFKWTWQLFLSDPFIFLFWVFISVTVFLWG
                     RGLFCGWLCPFGSLSELLYKIGGWIGLGRFQRALPKALHDKLKWIKYAVFFGLLGVSL
                     FSMTLAEQLAEVEPFKTTFLVGMFNRSWPYTLFAGGILGISIFIERPFCKYLCPLGAA
                     LAVPSTFRWFGLQRKNECNRCEACAKGCGSLAIDKHGRIDQRECLLCLDCMVLYTDDH
                     ACPPLAQERKYREKNALPLTAISPAGYFLPLPETTARSLPARKIAVQDLSVDPRMPSD
                     PATPACATPRNFIDAVRVEIIEHLWPWQRDQFNWQRTLVAFVL"
BASE COUNT         1612 a         2439 c         2224 g         1589 t
ORIGIN      
        1 agggcgccgt tcaggccgcc ataaaacgct gcagatgata atccccattg ccaaacaggg
       61 tattgatcac attgatgcgt ttcaggtaca tacccacgct catttcgtcg gtcatgccca
      121 tgccgccaaa aatctgtacg gcctcatggg ccaccttctg gcccttggtg gcgatggcat
      181 atttcagcgc ggcgatattg cgtgacgcct cggcatcgcc ggtagcactg gtgagcgtgg
      241 cacgcagcag cagggatttg cattccgcat gcagggtgaa catgtccacc atgcgatgct
      301 gcagcgcctg gaacacggcg atgggtacgc caaactgctt gcgggtcttg gtgtattcca
      361 ccgtttccct atacagcttt tccatcatgc ccacggcttc ggcacacagc gccagacggg
      421 cttcatccgc cacggcttcc agcaccgcca gggccttgcc ctcttcgccc agcagcgccg
      481 ctgcgggaac gctcacgttg gtgaaggtga cttccgccgc ccgctggcca tccagggtgg
      541 gatagtcccg cacgcttaca cctgccgcat ccgccggcac caccagcagg gaaacgccgc
      601 tggcatcgcg cgggttgcct gatgtgcgca ctgacacgat aaaggtgtct gccgtgccac
      661 cgtgcagcac catggctttg gcgccgttga caatgtagcg gtcgccgtct ttcttcgccg
      721 tggttttcac gtcgaaaaga tcaaagcggg attgcggctc gagataagcc agtgtcagca
      781 ggcggctgcc gtcgatgatg ccgccgatca gctccgcttt ctggctggcg ttacccgcgc
      841 gtttcagcac ggaacccgcc agcaccacgc aggatacata cggctccacc accaggccct
      901 tgccgaattc ctccatcatg atcatggttt cgatggggcc accgccgatg ccgccgtcct
      961 cttccgaaaa cggcacacac agccagccca gttctgcaaa ggtagcccag gtggcgcgat
     1021 cgaaaccgtc cggcagcgct acgtgcttct tgcggatgtc ataaccgtaa tcattgccta
     1081 tgaaccgggt gacgctgtcc gccagcaggc tttgttcttc actcagattg aaattcatga
     1141 tgcaactcct agaggcccag cacggctttg gcgatgatat ttttctgcac ttcgttggaa
     1201 ccgccataaa tggtggcggc gcggccatac agatacttgg ccgacaatgc ccaggcataa
     1261 tcgttgccca ccggcgcgtc tacattgagc gcatgggcgt aatggccgca cacttccacc
     1321 gccagttccg acagcgcctg ctggatttca gtaccgcgga ttttcaggat cgaggattcc
     1381 ggccccggcg caccgcctgc tgccacactg gcgatggcac gcagttcggt gtattccagc
     1441 gccagcagat caatttcgat tttcgccagc ttggcgcgga aatcattatc ttccgccagc
     1501 ggacgtccgc ccgacatttc cagcgccgcg atttcgcgca ggcgctgcac cgccttcttc
     1561 gactgtgcca caccggcaat cgccgtgcgc tcgtgcgtca gcaggtattt ggcataggtc
     1621 cagccctggt tttcctcacc gatgcggttt tccaccggca ccttgacgtt gtcgaaaaac
     1681 acttcattga gggaatgctg gttgtcgatg gaatcgatct tgcgcacggt gatgcccggc
     1741 gtcttcatgt cgatcagcag gaatgaaatg ccttcctgtt tcttggccga cggatcggtg
     1801 cgcaccaggc agaaaatcca gtcggccagc tgggcgtagg tggtccagat tttctggccg
     1861 ttcacgatgt agtggtcgcc atcgcgcacc gccttggtct gcagcgctgc cagatcggac
     1921 ccggcgcccg gctcggaata accctggcac caccatacgt tgctttgcag gatgtccggc
     1981 aggaaccgcg ccttctgcgc atcgttgccg aatttgtaga tcacgggccc gaccattttc
     2041 aggccgaacg gcaccacggc cggcgcgccc gcctcggcgc attcgttttc aaagatgtat
     2101 tttttggtga tgtcccaatc ggtaccgccg tacttcttcg gccagttcgg cgcaatccag
     2161 ccttgttcgt acagaatttt ctgccactgg atgatcagtt ccttgttgat atgtccgccg
     2221 accactgcat ccttgatggc gggggtcagt ttggacttca ggaacgcgcg gacttgatcg
     2281 cgaaaggcca gatcttcagg tttgaattgg atgtccattg ctctcctcga ctggtaagca
     2341 gcgggtagtg attgaacgaa gtgaaacgcg ggcaaatttt acgggccgcg cagggacggg
     2401 tcactgacag gaactgccag aagtcatgac cgaatacaac ctagcctaaa ggtaaacaga
     2461 tttccggcca gtggaaaggt ggaatgcgcc agaatgcggc cgcggctggc gcccacgggt
     2521 atcgggttgt aaagcgggtg aataagagga aaatggcgcc ccggagacga cttgaacgtc
     2581 cgacctgccc cttaggaggg ggctgctcta tccagctgag ctaccggggc gctgcgggcg
     2641 ctgattctaa caggttgttc cgccggtggg tatgtgccgc aggcggccca tgccattgcg
     2701 gcagctcgcg tttttcacta atctgccccc cgcctaactg cccaggggaa ttcccgtgcc
     2761 ttcacacgcc tccatagtgc tgttctgcag cctgctgtgc gctgccgttc tgcacccggc
     2821 cgccgcgcgc gccaccgaca gcccgtccta cggcaacgtc accgtgaccg aggtcaccag
     2881 catctacgac gccgacacct tcaaggtgga catccgccag tggccggcag tggtgggcag
     2941 tcgcatcgcc gttcgggtca agggcatcga cgctccggaa atgcagggca agtgcgagcg
     3001 ggaaaaggaa ctcgccagga aagccaagca gaccacggtg gcgatgctgc gggccggcaa
     3061 gaaaatcgaa ctgcgcaatc tgcaacggga caaatatttc cgcctgctgg ccgacgtcca
     3121 tgtggatggc aagaatctgg cgacaatcct gctgcaggcg ggccttgctt acccatataa
     3181 cggtggcacc aagaagagct ggtgtcaggt ttcgcaacct cgctgatcca acgtcaagcg
     3241 caacatacct ccaaagaggt gcactgccac tcctcttcat aacacctgca aaagcagtta
     3301 tttcgcttgc ataaaaatag ttttgtgcag ccctgcgcca acaccggaaa cagtccgttc
     3361 gtaaatgctc cagatcaagt attggctgat tgccctgcga caaaatgtcg cagtcaattc
     3421 agatcacgat ctcgcgcacg ctacaggagc ggacaaccca tgaagaagga tcaatccccg
     3481 aatcataacc tcgataccga cgcccccctg gacgccggac gccgcaaatt cctcacccag
     3541 accactgcgg tggcagggct tgccggtgcc ggcctgaccc tgggactggc cggttgcaag
     3601 gatgaatccg caccggcggc gggtgcaccg gctgccaaaa ccgaacctca cgcggcccct
     3661 gccggacata tcaagcccgg tgaactcgac acccattatg gcctctggag cggcggccac
     3721 accggggatc tgcgggttct gggcttcccg tcgggccggg aaatccaccg tattccctgc
     3781 ttcacccccg atgcgctggt gggctggggc atcaccaatg aatccaaggc catcatgggc
     3841 accaaagccg atggcaccct gaaatacacc gtgggcgaca gccaccatac ccatgcgtcc
     3901 tacaaggacg gcaactatga cggccggtac gcctggatca acgacaagat caacgcccgc
     3961 atcgcgcgca tccgtctgga tgtcttcgcc tgcgacaaga tcaccgaaat cccccacgtg
     4021 cagggctttc acggcatctt tcccgacaag cgcgatccgg tggatcccgc catcaattac
     4081 accacccggg tattctgcgg cgccgaattc cacattccgc tggtcaacga cggcaaaaac
     4141 atgggcgacg acagccagta ccgcgccctg ttcagctgcg tcgacgcgga gaccatggag
     4201 gtgcgctggc aggtgctgat cgacggcaac tgcgacctgg tggccacttc gttcgacggc
     4261 aagctggcgg catccaacca gtacaacact gaaaacggcc agcgttttga gaacatgatg
     4321 tcggcagaac gggacgcctg cattttcttc aacatcgccc gcatcgagca ggcagtgaag
     4381 gatggcaaat tcacccggat cggtgattcg aaaattccgc tggtggatgg cacccgcgcc
     4441 gccaacaaaa atccggccac tgcactgacc gcctatgtat cggtgccgaa aaatccgcac
     4501 ggcgtcaacg ccagccccga tggaaaatat ttcatctgcg ctggcaagct ttcccccacc
     4561 gccactgtga tcgaactggc gaaagtgctg gactggtttg acggcaaact gccggaactg
     4621 gacaagtcca tcgtggcaga agtggaactg ggcctggggc ccctgcacac cgcattcgac
     4681 ggccgtggca acgcctacac caccctgttc ctcgacagcc aggtagtgaa atggaacgtg
     4741 gaagccgcca tcaggttcca cggcggcgac aacaccgcca agtacgtggt ggaccgcctc
     4801 gatgtgcaat accagcccgg ccacatcaat gcctcgcagt cggaaaccat gttcgccgac
     4861 ggcaaatggc tggcggtggg ctgcaagttc tcgaaggacc gctacctgcc ggtaggcccg
     4921 ctgcatccgg agaacgagca gatcgtcgat atttccggtg acaagatggt gctgattgca
     4981 gatcacccgg tacgccccga accgcacgat ttcatcatct tcaagcggga tctggtgcgc
     5041 accaaacagg tgtacaacat ggctgacttc cccaacgcgg tgaagagttc cagcgagagc
     5101 ggtgtgttcc gggaaggcaa caaggttacg gtgaagatga cttcgcaggc gccgattttc
     5161 agcctgcgcg atttcactct gaagctgggt gatgaagtca cgctgatcct caccaacctg
     5221 gacaacatcg aggatctgac ccacggcttt gcgattgaaa aatacaacat caatttcatc
     5281 gtcaacccgc aggaaaccgc atccgtcacg ttcaaggccg acaagcccgg ggtgttctgg
     5341 tgttactgct cgcatttctg ccacgcattg cacctggaaa tgcgctcgcg catgatggta
     5401 gaggcctgag catcagcggg atctgtacca ggaggggcgc aacgcccctc cacaaccttc
     5461 cggccagccg cgacggacgc gccactgacc cattgaaccg ccggcggaat cgcccaatga
     5521 acacactgca agcactgcca aaattactgc tgctgttgtc gctgctgtgg acaacgcaaa
     5581 tggccctggc aggtgcctat gaagccaaac tgagcgaaga aatcttcagc gcgccggatc
     5641 tgtgcgcgca tgctccctgc accgatgtta ttcccggtgc cactgcgttt tcccagcgca
     5701 agggccaacc gccctatgtg gacgcctttg ccgatgtgaa tggcaagcgc gaactgatcg
     5761 gctatgtgat gctgtccacc gacatcaccg acacgcccgc ctattccggc aaaccggtga
     5821 tcacgctgat cggcatggac accaccggca agttcaccgg cgccaaggtg ctgaagcact
     5881 ccgaacccat cctgctgctg ggcattccgg aaacggcgct gacgaatttt ctcaaccagt
     5941 atgtcggcct gtttgccggc gacagcattg aagtgggcca cacgcggccg gatcaggaca
     6001 gcatcggcct ggacggcatt tccggcgcca ccgtcaccgt gattgcgcag aatcaggtga
     6061 tgctggcctc ggccactgca gtcgcgcgcc agaccggcat catcgaaccc accatccgca
     6121 accaggcaac atttgccgca gccggcaaac cgcaggactg gaacagcatg gtggccgaag
     6181 gcagtgtgca gcaactgcgg gtggagccgg aacaggtggg tctgcccgcc agcgccaccc
     6241 cgttcatcga gctgtggttc ggctatctga accagcccga cattggctcc gccattcttg
     6301 gcgacaacgg ctatcgcaat ctgatgctgc aattgaaaga cggcgaacat gcgctgtttg
     6361 tgatccgcac tgacggcggc gaatcgttca aagggtcggg ctttgtgcgc ggtggtattt
     6421 atgatcgcgt gcaggtacgg caggacacgg attcattcac attccgtgat cgcgattatc
     6481 tgaatctcta cagcattgac gttgcaggcg cgcctgccta caccgaatcc gccatctaca
     6541 ttatccgctc accggcgttt tccggcgcct atccatggaa gttcgtgttt ctgggcaatc
     6601 tggtcgacaa ggccaccggc acgcgcacct tcaccaattt tgatgcggaa tactggttgc
     6661 cggcgcgcta tctgcaaggc ggcagaccgg acgtggtccg ccccgatccg acctggctga
     6721 aaatctggaa aaaccgcctg cccgaaatca tcctgtttac actgtttctc attgcggtgg
     6781 gagtggtgta cagccagcgc aatcgcctga cccggcgctc gaaccggcac aacaaatggc
     6841 cggtcaacgg tttcaaatat gtggcctggg tgatcagcgt gaccctcgtt ggttttggat
     6901 tactggcaca gccctcggta acgcagattc tgacgtggtt ccactcgttg ctgttcaaat
     6961 ggacctggca gctgttcctg tccgatccgt tcatttttct gttctgggta ttcatttccg
     7021 tcaccgtatt cctgtgggga cgcgggctgt tctgcggctg gctgtgtccg ttcggatcgc
     7081 tgtcggaact gctgtacaag atcggcggct ggatcggcct gggccggttt cagcgcgcgc
     7141 tgcccaaggc actgcacgac aaactgaaat ggatcaagta cgcggtattt ttcggcctgc
     7201 tgggcgtgtc gctgttttcc atgaccctgg cggaacagtt ggccgaagtg gaaccgttca
     7261 agaccacgtt tctggtgggc atgttcaatc gcagctggcc ttacacgctg tttgccggcg
     7321 gcattctggg tatctctatt ttcattgagc ggccattctg caaatacctg tgcccgctcg
     7381 gcgcggcact ggcagtaccc agcaccttcc gctggttcgg cctgcaacgc aaaaatgaat
     7441 gcaaccgctg cgaagcctgc gcgaaaggat gcggctcgct cgccatcgat aaacacggcc
     7501 gcatcgacca gcgcgaatgc ctgttgtgtc tggattgcat ggtgctgtac accgacgatc
     7561 acgcctgccc gccgctggcg caggagcgta aataccgtga gaaaaatgcc ctgccgctga
     7621 cggccatcag tcccgctggc tatttcctgc cactgccgga aaccacggcg cgttcattgc
     7681 cggcgcgcaa gattgccgtg caggatctgt cggtcgatcc gcgcatgccc agcgacccgg
     7741 ccacaccggc ctgcgccacg ccgcgcaact ttattgatgc cgtgcgggtg gaaatcatcg
     7801 agcatctgtg gccgtggcag cgcgatcagt tcaactggca acgcacgctg gtggcttttg
     7861 ttct
//
LOCUS       DASUEG010000254         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1397683, whole genome shotgun sequence.
ACCESSION   DASUEG010000254 DASUEG010000000
VERSION     DASUEG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397683"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3692
                     /locus_tag="VFX11_04750"
     CDS             <1..3692
                     /locus_tag="VFX11_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4937959.1"
                     /translation="VRFPFVYTIVALTAVAFRDGALRAGLHGNLVVLSLGLLQLAGVI
                     DLSGVVSEAGAKAMWAAGAVTCLFPTLMGMAVENLREQQAAVNSLTERLHLATRSVGM
                     GVWDWNLRTRELHLDSQTQQLYELDRGLIRGDLDIWLHRVHPEDQARTEQELRAAIAG
                     LAEYNSEFRVLRQDGSVRHLHAAALSVRDSQGRVQRLVGLNWDVTQERQAERALRDAR
                     AQLQGVISAASEFSIIATDTRGIITLFSVGAERMLGYQASELVGICSPAQLHLADELV
                     TRGLELSAEYGRPIGGFDVFVENARHGKVDAREWTYVRKDRTTLPVKLVVSPISDDQG
                     HITGFLGVAQDITAQKLAQNTLLTTNRVLEHQISVAQRSRQEFESLFALAPGALLVVD
                     AAGHIIKANARAHDQFDYPQGSLLGVSVEDLIPFDRRHRHQQLREQYMQAPAPRLMAA
                     QRVLAALKRDGTQFLAEITLSPLRLNGMQCTIAIVRDVTEQRLAQEALALAKEQAESA
                     SRAKSEFVANMSHEIRTPLNAVLGTAQLLEKLTLNEQQRRYVDMIRSAGQSLLGILND
                     ILDFSKIEAGRMDLARVDFALDEVLANLATLMALNAGDKDIELAIGVAPGTPSHFRGD
                     PLRIQQILVNLVGNAIKFTERGEVVLRVALQSRQGDQAVLAFSVRDTGIGMTQEQQAR
                     LFSAFSQADTSMTRRFGGTGLGLVICKRLIELMQGEISLSSSAGVGTEFRFTLHLETA
                     APVLSLVPPPSERRVLVVDDNPTASTILTELIRSRGWQAECVNSVDAALACLRGAPDT
                     RPDTLLVDWKLPGAEVLAHLPDSAGAPRRILMVSSYGRERLQNPAHQWQGLLVKPITA
                     AYLFDTLLSTTPRRPDGSAGASPDAGLNGLRVLLVEDNPLNQTVACGLLEHMGALVDV
                     MENGRQAVDWLREHRDDYHVILMDVQMPVMDGFTATRLIRDELKLDLPVIAMSAGVML
                     PEQQECLASGMNDFIGKPIDYLHMRDTIARHTRRGRPHMPDVVKPAAPTATPVALSAP
                     AGATHFAPDRMLGFVRGKPNRLREILAMIDAVIAAGSEPVEEGRRLLAAGQTADAARH
                     FHTLKGSLGNLGAAQACEIAQQLEQAIKADQRDQWEPLLKEATVRMDAMAAAARAWRQ
                     AHPDLHAMTDPATPRPTDSGGPDPARLQELRLRLAENNIVACELYARLRDALASRLDE
                     SRLARLDQAIQRLDFLTARQLLPEA"
BASE COUNT          708 a         1301 c         1278 g          656 t
ORIGIN      
        1 atgtccgctt ccccttcgtc tacaccatcg tggcactgac ggcagtggcc ttccgcgacg
       61 gtgcgctgag ggcgggactg cacggcaatc tggtggtgct gtcgctgggc ctgctgcagc
      121 tggcaggggt tatcgatctg agtggcgtgg tcagtgaggc gggtgccaag gccatgtggg
      181 cggcaggcgc ggtcacctgc ctgttcccca ccctgatggg catggcggtg gaaaacctgc
      241 gcgagcagca ggccgccgtc aacagcctga ccgagcgtct gcacctggcc acgcgctcgg
      301 tgggcatggg cgtgtgggac tggaatctgc ggacccgtga gctgcatctg gacagccaga
      361 cccagcagct gtacgaactc gaccgcggcc tgatcagggg tgatctggat atctggctac
      421 accgggtaca cccggaagat caggcccgta ccgagcagga actgcgcgcc gccatcgcgg
      481 ggctggcgga atacaactcg gaattccgcg tcctgcgcca ggacggcagc gtgcgccacc
      541 tgcatgcagc ggcactgtcg gtacgtgaca gccagggccg ggtgcagcgc ctggtgggtc
      601 tgaactggga tgtgacccag gagcgacagg cggaacgggc cctgcgcgac gcccgcgccc
      661 agctgcaggg ggttatcagt gccgccagcg aattctcgat catcgccacc gacacccggg
      721 gcatcatcac cctgttcagt gtgggtgccg aacgcatgct gggttaccag gcatctgaac
      781 tggtggggat ctgttcgccg gcgcaactgc atctggcgga cgaactggtg acgcgggggc
      841 tggaactcag tgccgaatac ggccggccca tcgggggctt cgacgtgttt gtcgagaacg
      901 cccggcatgg caaggtggat gcgcgggaat ggacttacgt gcgcaaggac cgcaccacgc
      961 tgccggtgaa actggtggtg agccccatca gcgacgatca gggccatatt accggcttcc
     1021 tgggagtggc ccaggacatt actgcgcaga aactggccca gaacaccctg ctgaccacca
     1081 accgggtgct ggagcaccag atcagcgtgg cgcagagatc acggcaggaa ttcgaatccc
     1141 tgtttgcact ggcaccgggc gcgctgctgg tggtggatgc cgccggccac atcatcaagg
     1201 ccaacgcccg cgcccacgac cagttcgatt atccccaggg cagtctgctg ggggtgtcgg
     1261 tggaagacct gatccccttc gaccggcgtc accgccacca gcaactgcgg gaacaataca
     1321 tgcaggcgcc ggcgccgcgc ctgatggccg cacagcgggt actggcggcc ctgaaacggg
     1381 atggcaccca gttcctggca gaaattaccc tgagcccgct gcggctcaac ggcatgcagt
     1441 gcaccatcgc catcgtgcgt gacgtcaccg agcagcgcct ggcccaggaa gccctggcac
     1501 tggcgaagga acaggccgaa agtgccagcc gcgccaagag cgaattcgtt gccaacatga
     1561 gccacgagat ccgtacgccg ctcaatgccg tgctggggac ggcgcagttg ctggaaaagc
     1621 tgaccctgaa cgaacagcag cgccgctatg tcgacatgat ccgcagcgcc ggtcagtccc
     1681 tgctcggcat cctcaacgac atccttgatt tctccaagat cgaagctggc cgcatggatc
     1741 tggcgcgggt ggacttcgcc ctggacgaag tgctggccaa cctggcgacc ctgatggccc
     1801 tcaacgccgg cgacaaggat atcgagctgg ccatcggcgt tgcgcctggt acgccctcgc
     1861 atttccgcgg cgaccctctg cgcatccagc agatcctggt gaatctggtg ggtaacgcca
     1921 tcaagttcac ggaacgggga gaagtggtgc tgcgggtggc attgcagtcg cgccagggcg
     1981 accaggccgt gctggcattc agtgtgcgcg acaccggaat cggcatgacg caggagcagc
     2041 aggcacgcct gttctcggcg ttttcccagg cagacacctc catgacccgg cgctttggcg
     2101 gtaccgggct ggggctggtg atctgcaagc ggttgatcga attgatgcag ggcgagatca
     2161 gcctcagcag cagtgccggc gtcggcaccg aattccgctt caccctgcac ctggaaaccg
     2221 ccgccccggt cctgtcgctg gtgccacccc cgtctgagcg tcgggtgctg gtggtggatg
     2281 acaaccccac cgccagtacc atcctcaccg aactgatccg cagccggggc tggcaggccg
     2341 aatgtgtgaa ttccgtggac gcggcgctgg catgcctgcg cggcgcgccg gacacccgcc
     2401 cggacaccct gctggtggac tggaaactgc cgggcgcaga agtcctggcc catctgccgg
     2461 acagtgccgg ggcgccgcgt cgcatcctga tggtgagcag ctacggtcgt gaacgcctgc
     2521 agaacccggc gcaccagtgg cagggcctgc tggtgaaacc gatcaccgcc gcgtacctgt
     2581 tcgacaccct gttgtccacc acgccgcgcc gcccggatgg cagtgccggt gcttcccccg
     2641 atgcaggcct gaatggcttg cgggtgttgc tggtggaaga caatcccctc aaccagaccg
     2701 tggcctgcgg gctgctggaa cacatgggtg cgctggtgga cgtaatggaa aacggtcgcc
     2761 aggcagtgga ctggttgcgg gaacaccggg acgactacca cgtgattctg atggatgtgc
     2821 agatgccggt gatggatgga ttcaccgcca cccggctgat ccgcgatgaa ctgaaactgg
     2881 atctgccggt gatcgccatg agcgccggcg tcatgctgcc ggaacagcag gagtgtctgg
     2941 ccagtggcat gaacgacttt atcggcaagc cgattgatta cctgcacatg cgtgacacca
     3001 ttgcccgtca tacccgcagg ggcaggcccc acatgcccga cgttgtcaaa ccggcagcgc
     3061 caaccgccac cccggtggcg ttgtctgccc cggccggcgc cacccacttt gcccccgacc
     3121 ggatgctggg attcgtgcgt ggcaaaccca accggctgcg ggaaatcctc gccatgatcg
     3181 atgctgtcat cgccgccggc agtgaaccgg tggaggaagg tcgccgtctg ctggccgcgg
     3241 gccagaccgc ggacgctgcc cggcacttcc acactctcaa gggttccctc ggcaacctgg
     3301 gggccgccca ggcctgtgaa atcgcccagc aactggaaca ggccatcaag gcagaccagc
     3361 gcgaccagtg ggaacccctt ctaaaggaag cgaccgtgcg gatggacgcc atggccgccg
     3421 ccgcccgcgc ctggcggcag gcccacccgg acctgcacgc catgaccgac cctgccacgc
     3481 cccggcccac cgactccggc ggccccgatc cggcccgcct gcaggaactg cggctgcggc
     3541 tggcggaaaa caatatcgtg gcgtgtgaac tctacgcccg cctgcgcgat gcgctggcgt
     3601 cacggctgga cgaatcccgc ctggcgcgac tcgaccaggc catccagcgc ctcgattttc
     3661 tcaccgcccg acagctgctg ccggaggcct gacggcccgc gcccctcatt tgacttcccc
     3721 tgacccgggt ctatacctaa aacacggcgc ggcgccagca agcgcggctg cgaccacctg
     3781 ctcaattcac agggactgat ggcgttttct tatgaaaaag tcggtattac ccgcgctggc
     3841 cggactgctg gttccgtccc ttgtccttct gatcctggcg ttcaatagca cttccctcac
     3901 ttactggcac tggaccggca ttctgatagc cctggtggct gcc
//
LOCUS       DASUEG010000255         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1041459, whole genome shotgun sequence.
ACCESSION   DASUEG010000255 DASUEG010000000
VERSION     DASUEG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041459"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFX11_04755"
     CDS             <1..731
                     /locus_tag="VFX11_04755"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX4937960.1"
                     /translation="QSSTSSAQVMTSCWKEFADREGVVVAYPQALGFPTTWDAGDFCC
                     EPRGHDDEGFALHLVQCLMNPKKSRLELDPQRVYAVGLSNGGAMAGKLACSHSDVFAG
                     VALASQSFPYSDTESCRAPDEMGWKKAAFPVVEVRGKWDFVVPYYISLGWSLTARTSL
                     KRWAETNQCAGEPLVSDACDQPSAGPGCVRGQGFCATYDNCEGGVAVTQCSLHDDHLV
                     YVNPQRYNQCDAAWEQFQRVPVSR"
     gene            816..>2281
                     /locus_tag="VFX11_04760"
     CDS             816..>2281
                     /locus_tag="VFX11_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4937961.1"
                     /translation="MLIRLRRRLVMMLPALLLALYAVWTSSPWGMALEQRLGLEMLFQ
                     LRGERPAPPAVVVAAVTRESAQALGLPDKPHQWSRSVHARAVERLHALGARLLVFDIF
                     FEQPRDDAGDQAFANALRNAGNVLLFARSDRQLIELGNGTTADRQVLLQPLPPLAAAA
                     LGTAPLILPKVPARIDRFFLRHPALPDHATLPVRAYMQLHADAVLPAVDALLYNFYGP
                     PRSITTIEYADLIQQPQWVATHVRDAVVFIGYSASYQHDQRDGFYTAFTAQSGLDISG
                     VELAATAFANLQDDSWLRELPPLASVVLVWLLGLLVFALTRTLAPVTSAVLVLLVAAG
                     VSLLCFVLFTTQYLWLPWFNGVLILAPLAGGIGMWLRSRELHLQKMRLQQAFGKYLPA
                     EEIRRLVEQHELPASQEHHHSVCLVTDAQGYSRLSEQLAPAELARLMQDYYSAVIGAI
                     RNGKGLISDVAGDGIIALWPHLEPAAAWSALAPVIREI"
BASE COUNT          386 a          665 c          723 g          507 t
ORIGIN      
        1 gccagagttc aacttcatcc gcccaggtca tgacatcctg ctggaaagaa tttgcagatc
       61 gcgaaggcgt tgtggtggca tatccacagg cgctgggatt tcccactacc tgggacgcgg
      121 gtgatttctg ctgcgagccg cgcggacatg acgacgaagg ttttgcattg catctggtgc
      181 agtgtctgat gaatcccaaa aagtccaggc tggagctcga tccgcagcgg gtatatgcgg
      241 tgggcctttc caacggcgga gcaatggccg gcaaactcgc ttgcagtcat tctgatgttt
      301 ttgccggtgt tgcgctggcg tcgcaaagtt ttccctattc cgataccgaa tcctgccgcg
      361 caccggatga aatgggctgg aaaaaagcgg ccttcccggt agtggaggtg cgcggcaaat
      421 gggatttcgt ggtgccctat tacatatcgc tggggtggtc cctgacggcg cgaaccagtc
      481 tgaaacgctg ggcggagacg aatcagtgtg cgggtgagcc gctggtgtcg gatgcctgcg
      541 atcaacccag cgcaggtccc ggctgcgtgc gaggacaagg attctgtgcc acctacgaca
      601 actgtgaagg tggtgtggcg gtcacccagt gcagcctgca tgacgaccat ctggtgtacg
      661 tcaatcccca gcgctacaac cagtgtgacg ctgcctggga gcaattccag cgcgttcctg
      721 tcagtcgctg agtgcattgc ggcgtgcacc ggcatttttc ctggccggga ttgacgctat
      781 agtgtcggct cttcccagaa gcaggatgaa ttcccgtgct tatccgttta cggcgccggc
      841 tggtcatgat gctgccggcg ctgctgttgg cgctgtatgc agtatggacc agctcgccct
      901 gggggatggc gctggagcag cggctgggtc tggaaatgct gtttcagttg cggggggaaa
      961 ggccggcgcc gccagcggtg gtggtggctg ctgtcacccg ggaaagtgca caagccctgg
     1021 gtttgccgga caagccgcac cagtggtccc gttccgtgca tgcccgggcg gtggagcgat
     1081 tgcatgcact gggcgcaagg ctgctggtat tcgacatctt tttcgaacag ccccgggatg
     1141 acgcaggtga ccaggcattt gccaacgcgt tgcgaaatgc gggcaatgtg ttgctgtttg
     1201 cgcgctcgga ccggcagctg atcgaactgg gcaatggcac cactgctgat cgtcaggtgt
     1261 tgctgcaacc cctgccaccg ctggctgctg ctgcactggg taccgcgcca ctgatcctgc
     1321 cgaaagtacc cgcccgcatc gaccgtttct ttttgcgcca tcccgccctg ccagaccatg
     1381 ccacgctgcc ggtgcgcgcg tacatgcagt tgcatgccga tgccgttctg ccagccgtcg
     1441 atgcactgct ttacaacttt tatggaccgc cccgttccat taccaccatc gagtatgcgg
     1501 atctgatcca gcagccgcag tgggtagcga cccatgtgcg cgatgcggtg gtgttcatcg
     1561 gctattccgc cagttaccag catgaccagc gtgacggctt ctacaccgca ttcactgcgc
     1621 agagtggtct cgacatcagc ggcgttgaac tggcggccac cgcttttgcc aacctgcagg
     1681 atgattcctg gttgcgggag ttgcccccgc tcgcaagtgt ggtgctggtc tggctactgg
     1741 ggttgctggt attcgcgttg acgcgcacgc ttgcgccagt gacgtcggca gtgctggttc
     1801 tgctggtggc cgccggagtc agcctgcttt gctttgttct gttcacgacg cagtacctgt
     1861 ggttgccctg gttcaacggc gtgctgatac tggctcctct tgccggcggc attggcatgt
     1921 ggctgcgcag ccgggaattg catttgcaga aaatgcgttt gcagcaggcc ttcggcaagt
     1981 atctccccgc cgaggaaatc cgccgcctgg tggaacagca tgaacttccg gccagtcagg
     2041 agcatcatca cagtgtctgc ctggtgacgg atgcgcaggg ctactcgcgc ctgtccgagc
     2101 aactggcgcc ggctgaactg gctcgcctga tgcaggatta ctactccgcc gtgattggcg
     2161 ccattcgcaa cggcaagggt ttgatttccg acgtggcggg cgacggcatc attgcgctct
     2221 ggccgcacct ggagcccgcc gctgcatggt ccgccctggc gcctgtcatc cgagaaatcc
     2281 a
//
LOCUS       DASUEG010000256         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_897128, whole genome shotgun sequence.
ACCESSION   DASUEG010000256 DASUEG010000000
VERSION     DASUEG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897128"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFX11_04765"
     CDS             <1..343
                     /locus_tag="VFX11_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4937962.1"
                     /translation="ATVVPPTKLPRTTPVSKVVRVDASRFAVEGDMLFADAHALRAAG
                     EALLPTLTSPVTVDLAAVEGVGSVGVSVLLCWMRAAEKLGKSLIFVNMPERMYDVSRV
                     SGLDEVIPCSH"
     gene            454..717
                     /locus_tag="VFX11_04770"
     CDS             454..717
                     /locus_tag="VFX11_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX4937963.1"
                     /translation="MISADDIKQRIESGLPGAEVQVRLDGNKCVVAVVSAAFEGQRAV
                     KKQQLVYGCLNDIIASGELHAVSMHTYTPAEWATQKKLGVPGL"
     gene            736..1998
                     /gene="murA"
                     /locus_tag="VFX11_04775"
     CDS             736..1998
                     /gene="murA"
                     /locus_tag="VFX11_04775"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4937964.1"
                     /translation="MDKLIITGGRRLDGEIRISGAKNSALPVLAATLLCSEPTKVCNL
                     PHLQDITTMIELLGQMGVDLIIDDKLNVEVNARTIKELNAPYELVKTMRASILVLGPL
                     VAHFGEAHVSLPGGCAIGSRPVDLHLKGLEAMGAEIVLEAGYIHAYAKKGLHGARIVL
                     DCVTVTGTENLMMAATLAKGQTILENAAREPEVVDLAECLNKMGADVKGAGTDTIVIN
                     GVDRLYGCDYTVIPDRIETGTYLVAGAISRGRVKLKQTRPELLDSVLLKLQDAGAHIS
                     TGPDWIELDMKGNRPKSVNIRTAPYPAFPTDMQAQFTALNCVAEGTGTIIETIFENRF
                     MHVPELSRMGANIVLEGNTAIVKGVESLTSAPVMATDLRASAGLVLAGLAADGDTLVD
                     RIYHIDRGYESIEEKLQRLGAHIQRVPS"
     gene            2012..2656
                     /gene="hisG"
                     /locus_tag="VFX11_04780"
     CDS             2012..2656
                     /gene="hisG"
                     /locus_tag="VFX11_04780"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4937965.1"
                     /translation="MTAKLRIALSKGRILDETLPLLKTAGIELLEEPSKSRKLIFPTT
                     DSNVEIIIIRATDVPTFVEHGAADIGVAGKDVLMEHGARGVYEPLDLKISRCKLMVAG
                     KVAPGPVSGRLRVATKFVNITKRYYAEKGEQVEVIKLYGSMELAPLVGLADKIVDIVD
                     TGNTLRANGLEPTELIAEISARLIVNKASMKTKHAAIQKLIDQLGVAVVNANRG"
BASE COUNT          581 a          808 c          862 g          477 t
ORIGIN      
        1 agcgacagtg gttccgccga cgaagctccc aaggacgacg ccagtgagta aggtggtgcg
       61 ggtggatgcc agccgcttcg cggtggaagg cgacatgctg tttgccgatg cgcacgcgct
      121 gcgggcggcc ggggaagccc tgctgccgac cctcaccagc ccggtaaccg tggatctggc
      181 ggcagtggaa ggcgtgggca gcgtcggcgt gtcggtactg ctgtgctgga tgcgggcggc
      241 ggagaaactc ggcaagtccc tgatttttgt gaacatgcct gagcgcatgt acgacgtcag
      301 ccgggtcagc ggtctggacg aagtgattcc ctgctcccac tgacgctgcc ggctgttcag
      361 cttggggccg cttttgatta caatggcggc ccgattcggc atcccgacat gcttcatcac
      421 gattttgcca taccagcaac aggatcccat ctcatgatca gtgcagacga catcaagcag
      481 cgcatcgaat ccggcctgcc gggcgcggaa gtgcaagtgc ggctggacgg aaacaagtgc
      541 gtggttgctg tcgtttcagc tgcattcgaa ggccagcgtg ccgtgaagaa acagcagctg
      601 gtttatggct gcctgaacga catcatcgcc agtggcgagc tgcacgcagt cagcatgcat
      661 acctataccc ccgccgaatg ggcgacccag aagaagctgg gcgtacccgg gttgtaatcc
      721 acacgaggcc tcatcatgga caaactgatt atcaccggag gcagacgtct cgacggtgaa
      781 atccgcattt ccggcgccaa gaactcggcc ttgccggtgc tggctgccac cctgctgtgc
      841 agcgagccca ccaaggtctg caacctgccg cacctgcagg acatcaccac catgatcgag
      901 ctgctcggtc agatgggggt ggatctgatc atcgacgaca aactgaatgt tgaagtcaac
      961 gcccgcacca tcaaggaact gaacgcgccc tatgaactgg tgaaaaccat gcgcgcctcg
     1021 atcctggtgc tgggcccgct ggtggcgcat ttcggcgaag cccacgtgtc gctgcctggt
     1081 ggttgcgcca tcggcagccg tccggtggac ctgcacctga aagggctgga agccatgggt
     1141 gccgagatcg tgctggaagc cggctacatt cacgcctacg ccaagaaagg cctgcacggc
     1201 gcccgcatcg tgctggactg cgtcaccgtt accggcaccg aaaacctgat gatggcggcg
     1261 accctggcca aagggcagac catcctggaa aacgccgccc gcgaaccgga agtggtggat
     1321 ctggcggagt gcctgaacaa gatgggcgcc gacgtgaaag gcgcgggcac cgacaccatc
     1381 gttatcaacg gcgtggatcg cttgtacggc tgcgactaca ccgtgattcc cgaccgcatc
     1441 gaaaccggca cctatctggt ggcgggcgcc atcagccggg gccgcgtgaa actgaaacag
     1501 acccgccctg aactgctgga ttccgtgctg ctgaaactgc aggacgccgg cgcccacatc
     1561 agcaccggcc ccgactggat cgagctggac atgaagggca accggcccaa atccgtcaac
     1621 atccgcaccg cgccctatcc cgcgtttccc accgacatgc aggcgcagtt caccgcgctg
     1681 aactgtgtgg cggaaggtac cggaaccatc atcgaaacca ttttcgaaaa ccgcttcatg
     1741 cacgtgccgg aactcagccg catgggcgcc aatatcgtgc tggaaggcaa caccgccatt
     1801 gttaaagggg tggagagcct gaccagcgcg ccggtgatgg cgaccgatct gcgcgcgtcg
     1861 gccggactgg tgctggcggg gctggcagcc gatggcgata cgctggtgga tcgcatctac
     1921 catatcgatc gcggctacga gagcatcgag gaaaaactgc agcggctggg cgcgcacatt
     1981 cagcgcgtgc cgtcgtaaac aggaacacgc catgactgca aaactgcgca ttgccctgtc
     2041 caagggccgc attctcgatg aaaccctgcc gttgctgaaa acggcaggga tcgagctgct
     2101 ggaagaaccc tcaaaaagcc gcaaactgat tttccccacc actgattcca atgtggagat
     2161 catcatcatc cgcgcaaccg acgtgccgac gtttgtcgaa catggcgcgg cggatatcgg
     2221 cgtggccggc aaggatgtgc tgatggagca cggagcgcgc ggcgtgtatg aaccgctgga
     2281 cctgaaaatc tcgcgctgca agctgatggt ggcgggaaag gtggcgccgg gcccggtgag
     2341 cgggcgtttg cgcgtggcca ccaagttcgt caacatcacc aaacgctact acgccgaaaa
     2401 gggcgagcag gtggaagtga tcaagctgta cggctcgatg gagctggcgc cgctggtggg
     2461 cctggcggac aagatcgtgg atatcgtcga cactggcaac acgctgcggg ccaacggact
     2521 ggaaccgacg gaactgattg cggaaatcag tgcgcggctg atcgtgaaca aggcgtcgat
     2581 gaaaaccaag cacgctgcca ttcagaagct gattgatcag ctgggtgtgg cggtggtgaa
     2641 cgcgaatcgt ggttaattcc gtgagccggg tctgtttgcc tttcaagaca cgctgttgat
     2701 acgtccctgt acgctcgaca gcgaccat
//
LOCUS       DASUEG010000257         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_94687, whole genome shotgun sequence.
ACCESSION   DASUEG010000257 DASUEG010000000
VERSION     DASUEG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94687"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFX11_04785"
     CDS             <1..491
                     /locus_tag="VFX11_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX4937966.1"
                     /translation="SSMMMTIWLIMTALGFGAIVERAGLLNRVITPLINRTKSVAGAV
                     TALVGTCVASNALTSDQYISIVLPARMFRKTFEDRRLAPVMLSRVVGDSATVTSPLIP
                     WNSCGAYMSAALGVSAISFAPYCFFNLLNPLLTVIFTAVGFRVLRAVVPQKSVTSAEA
                     GS"
     gene            531..1958
                     /gene="arcD"
                     /locus_tag="VFX11_04790"
     CDS             531..1958
                     /gene="arcD"
                     /locus_tag="VFX11_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEX4937967.1"
                     /translation="MPEASNKLKLGALIALVVGSMVGGGIFSLPQNIAASASAGATLI
                     GWLITGVGMLTLAFVFQTLANRKPQLDGGVYAFAKAGFGDYMGFSSAWGYWISAWIGN
                     VSYMVLLFSTLGYFFPVFGEGNTPVAIGCASVLLWLLHFLVLRGIREAAFINTITTIA
                     KMVPLALFIVLAAVAFKLDVYTSDFWGTGNTELGSVMEQVRKMMLVTVWVFIGIEGAS
                     IFSARAEKRSDVGKATVIGFIGVLLLLVLVNLLSQGIMAQAQLAGLKNPSMAAVLEQV
                     VGAWGAKLIGIGLLVSLAGALLSWTLLCAEIIFSAAKDHTMPEFLRKENGNHVPANAL
                     WLTNGLIQLFLIITLFNSSTYLSLLYLATSMILVPYLWSAAYAVLLAFRGETYETDAG
                     QRNKDLFIAVLATVYAIWLIYAAGVQYLLLSALLYAPGAILFAKAKHELGQPIFTNLE
                     KLIFIGVLIGAGIAAYGLYAGFLSL"
     gene            1974..3227
                     /gene="arcA"
                     /locus_tag="VFX11_04795"
     CDS             1974..3227
                     /gene="arcA"
                     /locus_tag="VFX11_04795"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX4937968.1"
                     /translation="MSKIKLGVHSEAGKLHKVMVCSPGLAHLRLTPGNCDELLFDDVI
                     WVSQAKRDHFDFVTKMRERGVEVLEMHNLLTETVQNKDALKWILDRKLTANQVGVGLQ
                     DDMRGWLQSLEPRKLAEHLIGGVSVADLANALGQSKTISMLGDFIGLTSFVLPPLPNT
                     QFTRDTTCWIYGGVTLNPMYWPARRQETLLTTAIYKFHPDFVNAEFEVWYGNPDEDHG
                     AATLEGGDVMPVGNGVVLIGMGERTSRQAIGQVAQALFAKGAAQRVIVAGLPKSRAAM
                     HLDTVFSFCDRDLVTIFPEVVKQIVPFTLRPDESKTGGIDIRREEKSFLDVVAEALNL
                     KQLRVVQTGGDAYEAEREQWDDGNNVVCLEPGVVVGYDRNTYTNTLLRKAGVEVVTIS
                     ASELGRGRGGGHCMTCPILRDPVNY"
     gene            3258..4268
                     /locus_tag="VFX11_04800"
     CDS             3258..4268
                     /locus_tag="VFX11_04800"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX4937969.1"
                     /translation="MAFNLHNRNLLSLMHHTTRELKYLLDLSRDLKRAKYSGTEQQHL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDPTSSQIGHKETMKDTARVLGRMY
                     DAIEYRGYKQEIVEELAKFAGVPVFNGLTDEYHPTQMLADVLTMRENSDKPLHDISYA
                     YVGDARNNMGNSLLLIGAKLGMDVRIGAPKALWPADEFVAQCEQFAKESGARITLTED
                     PKQAVKDVDFIHTDVWVSMGEPIETWDSRIKLLLPYQVNKALMQATGTPRTKFMHCLP
                     AFHNTDTKVGKQIAEKYPHLAGGIEVTEEVFESPACIAFEQAENRMHTIKATMVATLG
                     NV"
     gene            4281..>4889
                     /locus_tag="VFX11_04805"
     CDS             4281..>4889
                     /locus_tag="VFX11_04805"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253860.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX4937970.1"
                     /translation="MRIVVALGGNALLRRGEAMTSENQRENVRIAAVELAKVAPGNEL
                     VIAHGNGPQVGLLALQGAAHNPDNPFPLDVLGAETEGMIGYMIEQELGNLLPFEVPFA
                     TILTQVEVDANDPAFKDPTKPIGPVYSKAEAEKLAKEKGWAIKPDGDKFRRVVASPRP
                     KRIFELRPIKWLLEHGTIVICAGGGGIPTVYDANRKLKGIEAV"
BASE COUNT          994 a         1390 c         1506 g          999 t
ORIGIN      
        1 tgtccagcat gatgatgacc atctggctga tcatgactgc attgggtttt ggcgcgattg
       61 tcgagcgcgc cggcctgctc aaccgggtca ttacgccgct gatcaatcgc accaaatcgg
      121 tagccggtgc ggtgaccgcg ctggtcggca cctgcgtggc ctcgaacgcg ctgacatctg
      181 atcagtacat atccatcgtg ttgccggcgc gcatgttccg taaaaccttc gaggatcgaa
      241 ggctggcgcc agtcatgctg tcccgcgtgg tgggtgattc ggccacggtg acgtcgccgc
      301 tgattccctg gaacagttgc ggggcttata tgtcggccgc tttgggtgtg tctgccatca
      361 gctttgcacc gtactgtttt ttcaatctgc tcaatccgtt gctgactgtg atattcactg
      421 ccgtgggatt ccgggtgctg cgtgcggtcg ttccgcaaaa aagcgtgacg tcggcagaag
      481 cgggcagctg aatacgccat tttctacctt attcaaacaa ggagacctac atgccagaag
      541 catcgaacaa gttgaagttg ggggcgttga ttgcgcttgt ggtgggctcg atggtgggcg
      601 gcggtatttt ctcgctgccg cagaacatcg cggccagcgc cagcgccggt gccacgctga
      661 tcggctggct gatcaccggt gtgggcatgc tgacgctggc atttgtattc cagacgctgg
      721 ccaatcgcaa gccgcagctg gacggcggtg tgtacgcctt cgccaaggcc ggtttcggcg
      781 attacatggg tttttcatcg gcctgggggt actggatcag cgcctggatc ggtaacgtca
      841 gctacatggt gctgctgttc agtacgctgg gttatttctt cccggtattc ggcgaaggca
      901 atacgccggt ggcgattggc tgcgcgtcgg tgctgttgtg gttgctgcat tttctggtac
      961 tgcgtggcat cagggaagcg gctttcatca acaccatcac gaccatcgcg aaaatggtgc
     1021 cgctggctct gttcatcgtg ctggctgccg ttgcgttcaa gctggatgtg tataccagtg
     1081 atttctgggg cacgggcaat accgaactgg gcagcgtgat ggagcaggtg cgcaagatga
     1141 tgctggtcac cgtgtgggtg ttcatcggca tcgaaggcgc cagcattttt tctgcccgcg
     1201 ccgaaaagcg cagcgatgtg ggcaaggcca ccgtgatcgg tttcatcggc gtgctgctgc
     1261 tgttggtgct ggtcaacctg ctgtcgcagg gcatcatggc gcaggcgcaa ctggcggggc
     1321 tgaagaatcc ctccatggcc gcggtgctgg aacaggtggt gggtgcctgg ggtgccaagc
     1381 tgatcggcat cggtctgctg gtgtcgctgg caggagcgct gctgtcctgg acgctgctgt
     1441 gcgcggaaat cattttctcc gccgccaagg atcacaccat gccggagttc ctgcgcaagg
     1501 aaaacggcaa tcatgtgccg gccaatgcgc tgtggctcac caacggtctg atccagctgt
     1561 tcctcatcat caccctgttc aacagctcca cttacctgag cctgctttac ctggccacgt
     1621 cgatgattct ggtgccgtac ctgtggtccg ctgcctatgc tgtgctgctg gcgtttcggg
     1681 gtgaaactta tgaaacggat gccggtcaac gcaacaagga tctgttcatc gccgtgctgg
     1741 caacggttta cgcgatatgg ctgatttatg ccgccggtgt gcagtacctg ttgctgtcgg
     1801 ccctgctgta tgcgcccggt gcgatcctgt tcgccaaggc gaaacacgaa ctcggccagc
     1861 cgatcttcac caacctcgag aaactgattt tcattggcgt tctgatcggt gcgggtattg
     1921 ccgcctacgg tctgtatgcc ggcttcctca gcctctaaga gaaggagttc aacatgtcca
     1981 aaataaaact cggcgttcat tctgaagcag gcaagctgca caaggtcatg gtgtgttcac
     2041 ccggccttgc ccatctgcgg ctgacccccg gcaattgcga tgaactgctg ttcgacgatg
     2101 ttatctgggt gagccaggcc aagcgcgatc acttcgattt tgtcaccaag atgcgcgagc
     2161 ggggcgtgga agtgctcgag atgcacaacc tgctgactga aaccgtgcag aacaaggacg
     2221 cactgaaatg gattctggat cgcaagctca ctgccaatca ggtgggcgtg ggcctgcagg
     2281 atgacatgcg cggctggctg cagagtctgg aaccgcgcaa gctggcggaa cacctgatcg
     2341 gcggcgtgtc ggtggcggat ctggccaatg cactgggcca gagcaaaacc atctccatgc
     2401 tgggcgattt tattggcctg accagttttg tgctgccgcc gctgcccaac acccagttca
     2461 cccgcgacac cacctgctgg atttacggcg gcgtcacgct gaatccgatg tactggcccg
     2521 cgcggcgcca ggaaaccctg ctcaccacgg cgatctacaa attccatccg gatttcgtca
     2581 acgccgaatt cgaagtgtgg tacggcaacc cggatgaaga tcacggcgcc gccacgctgg
     2641 aaggcggtga tgtcatgccg gtgggcaatg gcgtagtgct gatcggcatg ggcgagcgta
     2701 cctcgcgcca ggcgatcggt caggtggcac aggcactgtt tgccaaaggc gctgcgcaac
     2761 gcgtgattgt ggcgggtctg cccaagtccc gcgccgccat gcacctggat accgtgttca
     2821 gtttctgcga ccgcgatctg gtcaccatct tcccggaagt ggtgaagcag atcgtgccgt
     2881 tcacgctgcg tccggacgaa agcaagaccg gtggcatcga catccgccgc gaggaaaaat
     2941 ccttcctcga cgtggtggcc gaggcgctca acctgaaaca gctgcgcgtg gtgcagaccg
     3001 gcggcgacgc ctatgaagcc gaacgcgaac agtgggacga cggcaacaac gtcgtgtgcc
     3061 tggaacccgg cgtggtagtg ggttacgacc gcaataccta caccaacacc ttgctgcgca
     3121 aagccggcgt ggaagtggtg accatttccg ccagcgaact gggacgcgga cgtggcggcg
     3181 gccactgcat gacctgcccg atcctgcgcg accctgtcaa ttattgattc gatcactatt
     3241 tactgaagga ttagaccatg gctttcaatc tgcacaaccg caacctgctc agcctgatgc
     3301 atcacaccac ccgggagctg aaatacctgc tggacctgtc ccgcgatctc aaacgcgcga
     3361 aatattccgg cactgaacag cagcatctga aaggcaagaa catcgcgctg attttcgaaa
     3421 agacctccac ccgcacccgt tgcgcgtttg aagtggcggc gtacgaccag ggtgcaaacg
     3481 taacctatat cgacccgacg tcgtcgcaga tcggtcacaa ggaaacgatg aaagacactg
     3541 cccgcgtgct gggccgcatg tacgacgcca tcgaataccg tggctacaaa caggagatcg
     3601 tggaggagct ggccaagttc gccggcgtgc ccgtgttcaa cggtctgacc gacgaatatc
     3661 acccgacaca aatgctggca gacgtgctga ccatgcgcga aaacagcgac aagccgctgc
     3721 acgacatcag ctatgcctat gtgggcgacg cccgtaacaa catgggcaac tcgctgctgc
     3781 tgatcggcgc gaagctgggc atggatgtgc gcatcggcgc gcccaaggcg ttgtggcccg
     3841 ccgacgaatt cgtggcacag tgcgagcagt ttgccaagga aagcggcgct cgcatcacac
     3901 tgacagaaga tccgaagcag gcggtgaagg atgtggattt cattcacacc gacgtgtggg
     3961 tatccatggg tgagccgatc gaaacctggg acagtcgcat caagctgctg ctgccctatc
     4021 aggtgaacaa ggcactgatg caggcaacgg gtacgccgcg taccaagttc atgcactgtc
     4081 tgcctgcgtt ccataacacc gacaccaagg tgggcaagca gatcgcggaa aaatatccgc
     4141 atctggccgg cggtatcgaa gtgaccgagg aagtgttcga atccccggcc tgtatcgcgt
     4201 tcgagcaggc ggagaaccgc atgcacacca tcaaggccac catggttgcc accctgggta
     4261 acgtgtaagg agaacgcatc atgcgcatag tggttgcact cgggggtaac gccctgctgc
     4321 gtcgtggcga ggccatgaca tcggaaaacc agcgcgaaaa cgtgcgcatt gcggcggtgg
     4381 agctggccaa ggtcgcgccg ggcaacgagc tggtgattgc ccacggcaat ggtccgcagg
     4441 tgggcttgct ggcgctgcag ggcgccgcgc acaatccgga caatccgttt ccgctggatg
     4501 tgctgggcgc cgaaacggaa ggcatgatcg gctacatgat cgaacaggag ctgggcaact
     4561 tgctgccgtt cgaagtgccg ttcgccacca ttctcacaca ggtggaagtg gacgcgaacg
     4621 atccggcctt caaggatccc accaagccca tcggccccgt gtattccaaa gcggaagccg
     4681 aaaagctggc gaaggaaaaa ggctgggcga taaaacccga cggcgataaa ttccgtcgcg
     4741 tggtggccag cccgcgcccg aaacggattt tcgaactgcg cccgatcaag tggttgctgg
     4801 aacacggcac gattgtgatc tgcgccggtg gcggtggtat tcccaccgtg tacgatgcca
     4861 atcgcaaact gaaaggcatc gaagcggtg
//
LOCUS       DASUEG010000258         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_430116, whole genome shotgun sequence.
ACCESSION   DASUEG010000258 DASUEG010000000
VERSION     DASUEG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430116"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1873)
                     /locus_tag="VFX11_04810"
     CDS             complement(206..1873)
                     /locus_tag="VFX11_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4937971.1"
                     /translation="MMEILKAGKDLRRLNELSNTLIKYGFGDLIRRMGLADSLERAGK
                     LIRYQMSEDFLHLQPHERARRAMEEMGPTFIKLGQILATRVDLFPAEWIDEFGKLQDE
                     VPAVPFADLQPQVERSLGQPLNRVFRVVDDKPLGTASMAQVHRATTVKGERVVLKIRK
                     PNIRDKIEADLRLLKYVAKLAESQSAELKRYSPFQIVREFERSLIRELDFSVECKNAE
                     RIAQNLKQFNFVKIPRIYWEWTCEDLNVQEFIEGIPARNVREIDAAGLDRKLIARRGA
                     KVAWHMMLVDGFFHADPHPGNILVLPRNQIAILDFGMVGKLSNSRRDQLMKLVRHAVF
                     REIPAAVRILQQWSNKTGNFEQLVSECEDFMQQYYGVPLDQLNIPQIITQAADTLREH
                     DIALPPDIAMLSKTCITLEGFGRLLYPQFDLMQEAEGLIRIWGKERYKPGNLVKKLGL
                     RAFNYVDSLFDDAEVTAATDHAPTLNGIDRGLVERLAKRQERLRYRQVQLMVAVGFLL
                     SSTLLMLLPEGPKLWGMHVVGIVLFLGSAVGIQWLLFLLWWGHRSTD"
     gene            complement(1982..>2238)
                     /locus_tag="VFX11_04815"
     CDS             complement(1982..>2238)
                     /locus_tag="VFX11_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937972.1"
                     /translation="GYVGFFDPVTQDVEQWAPADDALAHKRMDIKGRLASGRGYMRKN
                     SKAVVSNERRHQASLEQGNSKKPAPAGAKRTAAPGKRRKG"
BASE COUNT          397 a          680 c          694 g          467 t
ORIGIN      
        1 acagcaccgc cagcccggtg gccagccgga accgcaggct gcgatgccag gaaaaggccg
       61 gacggggcgg tccatgctcc tgcattggcg cctctgttgg ctgaaagtcg gacaattctc
      121 cgggattttc ttccatgtgc attccgtctg ttgtcgactt gtgcagtata ggtcggtctg
      181 gtggacctgg aatagcgacg gtggctcagt cggtgctgcg atgcccccac cagagcagga
      241 acagcagcca ttggatgccg acagcagaac ccaggaacaa gacgatgccc accacatgca
      301 tgccccagag cttggggcct tccggcagca gcatcagcag ggtggacgac agcagaaagc
      361 ccactgccac catcagctgc acctggcggt acctgagccg ctcctgccgc ttcgccagcc
      421 gttccaccag cccccggtcg atgccgttca gcgtcggcgc atgatcggtg gccgccgtca
      481 cttcggcgtc gtcgaacagg ctgtccacat aattgaacgc gcgcaggccg agcttcttca
      541 ccaggttgcc gggcttgtag cgctccttgc cccagatcct gatcaggcct tccgcttcct
      601 gcatcagatc gaactgcgga tacagcaggc ggccgaatcc ctccagtgtg atgcaggttt
      661 tgctgagcat ggcgatgtcc ggcggcagcg cgatatcgtg ttcgcgcagc gtgtcggcgg
      721 cctgggtgat gatctgcgga atgttgagct ggtccagggg cacgccgtaa tactgctgca
      781 tgaaatcttc gcattcgctg accagctgct cgaaattgcc ggtcttgttc gaccactgct
      841 gcaggatgcg cacggcggca ggaatttccc ggaacacggc gtggcgcacc agtttcatca
      901 actggtcgcg gcgggagttg gagagtttgc ccaccatgcc gaaatcgaga atggcgatct
      961 ggttgcgcgg cagcaccagg atgttgcccg gatgcggatc ggcgtggaaa aaaccgtcca
     1021 ccagcatcat gtgccaggcc accttggcac cgcgccgggc aatcagcttg cggtcgaggc
     1081 cggcggcgtc gatctcgcgc acgttgcggg ccggaattcc ctcgatgaat tcctgcacgt
     1141 tgagatcctc gcaggtccac tcccagtaga tgcgcgggat tttcacgaaa ttgaactgct
     1201 tgagattttg cgcgatgcgt tcggcgttct tgcactccac gctgaaatcc agttcgcgga
     1261 tcagtgagcg ttcgaattcg cgcacgatct ggaacgggct gtaacgtttc agctcggcgg
     1321 actggctttc cgccagcttg gccacgtatt tcagcagacg cagatcggct tcaatcttgt
     1381 cgcggatgtt gggcttgcgg attttcagca ccacgcgttc gccttttacc gtggtggcgc
     1441 gatgcacctg cgccatcgat gcggtgccca gcggcttgtc gtcaacgacg cggaacactc
     1501 ggttcagcgg ctggcccagc gagcgttcca cctgcggctg cagatcggca aagggcacgg
     1561 cgggcacttc gtcctgcagc ttgccgaatt cgtcgatcca ctcggcggga aacagatcca
     1621 cccgggtagc cagaatctgc cccagcttga tgaaggtggg gcccatttcc tccatggcgc
     1681 ggcgggcacg ttcgtggggt tgcagatgca ggaagtcttc agacatctgg taacggatca
     1741 gcttgccggc gcgctccagg ctgtcggcca ggcccatgcg gcggatgagg tcgccaaaac
     1801 cgtacttgat gagcgtgttg ctgagttcgt tcagtcggcg caggtcttta cctgccttga
     1861 gaatttccat catgagatga gcgtatccgg cagcgggtgg agtaactcgc tgattctagc
     1921 gcaatttggc cgggaaagcg gagcggcggc gacagagatc agccgccgcc agacacgcac
     1981 gtcagccttt gcgacgtttg ccgggcgctg ccgttcgttt ggccccggct ggcgcgggct
     2041 ttttgctgtt accctgttcc aggctggcct gatgccgtcg ctcgttggac accaccgcct
     2101 tgctgttctt gcgcatatag ccacgtccac ttgcgaggcg gcccttgatg tccatgcgct
     2161 tgtgggcaag cgcatcatct gccggcgccc attgttcgac gtcctgggtg accggatcaa
     2221 aaaatcccac gtaaccgc
//
LOCUS       DASUEG010000259         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_724, whole genome shotgun sequence.
ACCESSION   DASUEG010000259 DASUEG010000000
VERSION     DASUEG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..851
                     /locus_tag="VFX11_04820"
     CDS             231..851
                     /locus_tag="VFX11_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEX4937973.1"
                     /translation="MAGLDEVKGFLGCATPTAWLARAPENLDILLIDHAHCEKKAAST
                     ALNLLFRYVDKRNLLSSMSQLAREELLHFEQVVEMMERHGVQYGHLSPSRYAQGLRQH
                     IRPTEPDKLVDTLIVGAFIEARSCERFDALAPLLQANPATADIGKYYIFLLKSESRHY
                     EGYLALAREYSPVDIEDRIAFFRAVERELIETPDTDFRFHSGVPQA"
     gene            complement(867..1604)
                     /locus_tag="VFX11_04825"
     CDS             complement(867..1604)
                     /locus_tag="VFX11_04825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX4937974.1"
                     /translation="MAMANLFVSDLHLEDQRPDILRAFFRLLDEQKKDLQRIYLLGDI
                     FEVWLGDDTPSSCADALAEKLSGLAREGIKIFLLHGNRDFLIGSAYAARCGATLLHEP
                     VLLNVAGRPTALLHGDSLCTLDTDYMAFRKMVRDPQWIEQFLAKPLNERIAIGRSLRE
                     QSRQSAQQKQEYIMDVTPDEVLATLRDLKVELLIHGHTHRPAVHDLMLDGKAVQRLVL
                     GDWHTQGWYLLADDNDIQLKSFPFSGD"
     gene            complement(1607..2128)
                     /locus_tag="VFX11_04830"
     CDS             complement(1607..2128)
                     /locus_tag="VFX11_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4937975.1"
                     /translation="MEAILVILHTNMGDIKLELNKEKAPVTVENFVQYVNEGHYNGTI
                     FHRVIGNFMIQGGGFDKDMQQKPTRASIKNEANNGLKNEVGTIAMARTQDPHSASAQF
                     FINVTNNSFLNFSAENRQGWGYAVFGKVVEGMDVVNNIKGVKTATQGFHENVPVTPVI
                     IEKAEVVSAAKQL"
     gene            complement(2146..3003)
                     /gene="folD"
                     /locus_tag="VFX11_04835"
     CDS             complement(2146..3003)
                     /gene="folD"
                     /locus_tag="VFX11_04835"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX4937976.1"
                     /translation="MTTKIIDGTRIANQLKDQVKLRVQHRREQGLRAPGLAVILVGGD
                     PASKVYVNKKRQSCEEVGFVSRAYDLPESSSQADLLALIHELNADKSIDGILVQLPLP
                     KHIDDKAVINAILPDKDVDGFHPYNMGRLAQRIPLLRPCTPKGIMTLIESTGVELLGL
                     EAVVVGASNIVGRPMSLELLLAGCTVTTCHRFTRNLQEHVGRADLVVVAVGKAEFLPG
                     EWVKPGAIVIDVGINRCSDGKLRGDVAYTAAAERAGWITPVPGGVGPMTVATLLENTL
                     IAAEELHSR"
BASE COUNT          694 a          907 c          905 g          740 t
ORIGIN      
        1 cttggcaaaa taccagccga gccgaccgag aaggtgcccg atcctgccca ccacaggcag
       61 cggcagcaaa cccagcaact tcaacgtcgt cacaatgacg gtgcctttga tatacactcg
      121 ttccggttcc cgtggcagtc gattcggcgg aaagaatagg gcaaaaatcg ccatcgctct
      181 atgaaaaccg tataattcac gaccttgaat tcgtaacggt tggtaaaagt atggcaggtc
      241 tggacgaggt aaaggggttt ctgggttgcg cgactccgac ggcctggctt gcgcgtgcgc
      301 cggagaatct cgacatcctg ctgatcgatc atgcccactg cgaaaaaaag gcggcgtcca
      361 ccgcgctgaa cctgctgttc cgctatgtcg acaagcgcaa tctgctgtcc agcatgtcac
      421 aactggcccg ggaggagctg ctgcatttcg agcaggtggt ggaaatgatg gagcgtcatg
      481 gagtgcagta tggtcacctg tcgccgtcac gttacgccca gggcctgcgc cagcatatcc
      541 gtcccaccga gccggacaaa ctggtagaca cgctgattgt cggcgctttc atcgaagccc
      601 gttcctgtga gcgttttgat gcgctggcgc cattgctgca agccaatccg gccacagccg
      661 acatcggcaa atactatatt ttcctgctga aatccgagag tcgtcattac gaaggctatc
      721 tggcgctggc gcgggaatat tcacccgttg atatcgaaga ccggatcgcg tttttccgcg
      781 cagtggaaag ggaattgatc gaaacgccgg atacggattt ccgctttcac agcggagtgc
      841 cgcaggcgta gaggcggagt ccggaattag tcgccgctaa agggaaacga tttcagctgg
      901 atgtcattgt catcagccag cagataccag ccttgcgtgt gccagtcccc caacaccaga
      961 cgctgtaccg cttttccatc cagcatcaga tcatgcaccg ccgggcgatg ggtgtggccg
     1021 tgaatcagca gctccacttt taaatcacgc agggttgcca gcacttcgtc cggcgtaaca
     1081 tccatgatgt attcctgctt ctgctgcgcg ctctgtctgc tttgttcccg cagcgagcga
     1141 ccaatggcga tgcgttcatt cagtggtttc gccaggaatt gctcaatcca ctgtggatca
     1201 cgcaccattt tgcgaaacgc catgtaatcc gtatccagcg tgcagagcga atcgccgtgc
     1261 agcagggcag tggggcggcc ggctacgttc agcaacaccg gttcatgcag cagcgtcgcg
     1321 ccgcaacggg cggcataagc agaaccaatc agaaaatccc ggttgccgtg cagcaggaaa
     1381 atcttaatac cttcccgcgc caggcccgat aatttctccg ccagtgcatc ggcacaggac
     1441 gatggcgtat cgtcccccag ccagacctcg aaaatgtcac ccagcagata aatccgctgc
     1501 agatcttttt tctgttcatc cagcaaacgg aaaaacgccc ggaggatatc cgggcgctgg
     1561 tcttccagat gcaggtctga aacgaacagg ttggccatgg ccatctttac agttgcttgg
     1621 cggcgctgac cacttcagcc ttttcgatga tcacgggtgt cacgggcacg ttctcgtgga
     1681 aaccctgggt agcggttttc acgcccttga tgttgttcac cacgtccatg ccttcaacca
     1741 ctttgccgaa cacagcgtag ccccaaccct gacggttttc ggcagagaaa ttcaggaagc
     1801 tgttattggt gacgttaatg aagaattgcg cgctggcgga gtgggggtct tgcgtccgtg
     1861 ccatggcgat ggtgcccact tcatttttca gtccattgtt ggcttcgttt ttgatggaag
     1921 cacgggtggg cttctgctgc atgtccttgt cgaaaccgcc accctgaatc atgaagttgc
     1981 cgatcacacg gtggaaaatg gtgccgttat aatggccttc attcacgtac tggacgaagt
     2041 tttcgacggt gacgggggct ttctccttgt tcagttcaag tttgatatcg cccatgttgg
     2101 tgtgaagaat gacaagaata gcttccatca tatagttcct tcgttttagc gtgagtgaag
     2161 ttcttccgct gcaatcagcg tattttcgag caaagtggcg actgtcatcg ggccaacgcc
     2221 gccgggcacc ggcgtgatcc agccggcccg ctccgccgct gccgtatagg ccacatcgcc
     2281 gcgcaatttg ccatcgctgc aacgattgat gcccacgtca atgacgatag cgccaggttt
     2341 gacccattca cccggcagaa attccgcttt gcctacggca accaccacca gatccgcccg
     2401 acccacgtgc tcctggagat tgcgggtgaa gcgatggcag gttgtcaccg tgcagccggc
     2461 cagcagcagt tcaagcgaca tggggcgacc gacaatgttg gatgcaccca ctaccaccgc
     2521 ttccagcccc agcagttcaa cgccggtgga ctcgatcagg gtcatgatgc ctttgggcgt
     2581 gcagggccgc agcaggggaa tgcgttgcgc cagacgtccc atattgtaag gatggaagcc
     2641 atccacatcc ttgtccggca gaatggcgtt gatgacggcc ttgtcgtcga tatgcttggg
     2701 caatggcagt tgaaccagga tgccatcgat gcttttgtca gcattcagct catggatcag
     2761 ggcaagcaga tccgcctggc tgctggattc tggcaggtcg taagcgcggg aaacaaatcc
     2821 cacttcctcg cacgactgcc gttttttatt cacatacacc ttcgacgccg gatcgccccc
     2881 caccagaatg acggccagtc ccggtgcccg caaaccctgt tccctgcggt gctgaacgcg
     2941 cagttttacc tggtccttga gctggtttgc aatgcgggtg ccgtcgatga tcttggttgt
     3001 cataatatag agggagtagc gtcgtccgtt gggcggaggc tggaaaaggg cggccatttt
     3061 ctcactaatg gcccgatgtg gcaaagaaat cggtgcaatg tgctgatctt gttgatattt
     3121 atgaaaatgt ttgacgagta aatcagctat gtatattatg ctgccctcgc tactggaacg
     3181 gtatcgacac ggggtatagc gcagtctggt agcgcgcctg ctttgggagc aggatgtcgg
     3241 gggttc
//
LOCUS       DASUEG010000260         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_506454, whole genome shotgun sequence.
ACCESSION   DASUEG010000260 DASUEG010000000
VERSION     DASUEG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506454"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..882)
                     /locus_tag="VFX11_04840"
     CDS             complement(148..882)
                     /locus_tag="VFX11_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4937977.1"
                     /translation="MSKTQHPKKEEKQGARRIPRPQRELQMLEAAAVEFGRKGFEATS
                     MDDIAAACGVTKPMLYNYFGSKDGLYAAMINQAGSHLVKSIIALRELTDPVQRLHGAM
                     GVFLEFVERYRDSWRMVFSGKGFSGKGSSGQDQQGSIAGYRQQILIGTLYTLGQLRPA
                     GTSGEAARQHMEPYAWGLLGAGESIAQWWLATPGIGIEQARAAISKMIDATVVLARAE
                     LLAADLPTGKSEGKSEGKPKEKSAGT"
     gene            1014..1838
                     /locus_tag="VFX11_04845"
                     /pseudo
     CDS             1014..1838
                     /locus_tag="VFX11_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
     gene            1979..3715
                     /locus_tag="VFX11_04850"
     CDS             1979..3715
                     /locus_tag="VFX11_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4937978.1"
                     /translation="MSRQREVVSGAVRLAVRCDGDPAAPPVLLVHGYPDASQVWDAVA
                     DRLATEFLVIRYDVRGAGQSSAPAEARGYRLEQLSQDLQAVMDAVSPDRPVHLVGHDW
                     GSIQTWEAVTDPALKERIASFTSLSGPCLDHMGYWLRDKLRQPSPAHVLALMRQLIRS
                     WYVMLFHLPGTGLVWKLGLARLWPRLLRITEGIDVPGRPSQSRDGWNGVALYRANFLR
                     RLLLPRERIAVAPVQLVIAGNDAYVSAGIFDGLARWVPELYVKRVRGGHWLPLKQPTL
                     VAGLIADFIRARENPGGTQSRLTPVQGPLREFSGRQVLVTGAASGIGRATALAFAEQG
                     ASLILADLNLDGAERTALLARLHSVSAWACRVDVGDGVAMRTFADGVEDAFGLPDIII
                     NNAGIGMAGGLLHTSERDWERILRVNLWGLIHGIRLFGERMVQSGNGGHIVNVASAAT
                     FTPSRTFPAYATTKAAALMLSECARAEFSRFGIGVTAVCPGFVDTGIALSTHYTGVNA
                     DEQQRRREQAAKLYQRRGFTPEKVAAAIVGAVRRNRPLALVGVEAQGARWMSRFMPWA
                     ARLFARIDFSPN"
     gene            3768..4655
                     /locus_tag="VFX11_04855"
     CDS             3768..4655
                     /locus_tag="VFX11_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4937979.1"
                     /translation="MSETQHHHPLVARNVKFQWENTPHPWLPGDSFTSHFINVLHLLL
                     PEGELWFCRLYNQALPDIEDETLRADVKGFIRQEAIHSRSHQVVLDRYFADNGIVTEP
                     YTRRVRWLFTDLLGDHPFGIATERQWIKRAWLTFRLGLIAAIEHFTCVIGRWILECRG
                     LEEAGADPVMMDLLRWHGAEEVEHRTVAHDLFEQRGGGYVYRVMLMLLVGPMLLLLWF
                     LGTRFFLRQDPAADPNLKFIREWRRAARQDRLPTMAFLWLSVWRYLRPGYHPRDEAET
                     GQALDYLQRSPAALAATVR"
     gene            4725..>5124
                     /locus_tag="VFX11_04860"
     CDS             4725..>5124
                     /locus_tag="VFX11_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937980.1"
                     /translation="MKKIFAGALALTFVSQPIAAIPSSYIDPVWRPFLLLAGDGDPQR
                     ASDRLQRIKRLQTTMLNNTRYDTETQAFVDNLWRSMEKFDLDGFYGSLPDQKGRQREL
                     NVRGIWQDDAYLYERVPAAELSPQLTTLPLG"
BASE COUNT          865 a         1479 c         1690 g         1090 t
ORIGIN      
        1 gttacaccag tcagtcgctg gtgctgtggg ggccgcaagg gcagcctgtg gtgctgggcc
       61 ggcagaatat ggtgattttc ggctgacgtt tttagaggtg ccctggtttt ctgatgagcc
      121 agtcctgact ttgtgccggt acctgtttca ggtgccggct gatttctctt tcggtttccc
      181 ttctgacttc ccttcagact ttccggtcgg cagatccgct gccagcagtt ccgcccgtgc
      241 cagcaccacg gtggcgtcga tcatcttgct gatggccgcc cgggcctgct cgatgccgat
      301 gcccggcgtc gccagccacc actgcgcgat ggattccccc gcgcccagca atccccaggc
      361 atagggttcc atgtgctgcc gcgccgcctc gccgctggtg ccggcgggtc gcaactggcc
      421 gagggtgtac agcgtgccga tcagaatctg ctgccggtag ccggcgatgc tgccctgctg
      481 gtcctgtcct gaagatccct ttcctgaaaa ccccttgccc gaaaacacca tgcgccagct
      541 gtcgcgatag cgctccacaa attccaggaa cacgcccatg gcgccgtgca ggcgctgcac
      601 cggatcggtc agttcccgca acgcgatgat ggatttcacc agatgactgc cggcctggtt
      661 gatcatggcg gcgtacagcc cgtccttcga gccgaaatag ttgtacagca tgggtttggt
      721 gacaccgcag gcagccgcga tgtcgtccat cgaggtggcc tcgaagccct tgcggccgaa
      781 ttccacagca gcggcctcca gcatctgtag ctcgcgctgg ggccggggaa tgcgccgggc
      841 gccttgtttt tcttcttttt ttggatgctg cgtcttgctc atggagtggc tccgttgcct
      901 gctgccgttc aactttgctt gacagtttat tacccagtgg taagttatgt cgatgttact
      961 tttgggtaac atgatatttg gccggctcgg ccttttgact cagagggatt ccaatgaaca
     1021 gtacgacaga ggtgttgccg ggggtggatc tggcccggtt caaggcgctg cagcggctgg
     1081 cctacgattg cgccgagacg gtggcgcggg aactgcagcc gggggtgacg gaaaagcagg
     1141 tggcgcagaa gatgaaggtc tggctgctgg atcacggggt ggacgactgg tttcaccagc
     1201 ccttcgcctg gttcggcgat cgcaccgcct ttcgcggctt tagtgggttc aacccgcgtt
     1261 tcttcccgtc gtcgcgccgg ctggaggaga acatgcccta catcctcgac tgcgctccgg
     1321 tgcagggtgg cgcggtggcg gacatcggct attcgggctg ccttggccac aatccgatgc
     1381 tggaccagtt gatggacgat ctggcggcgc accgggatct gattttgcgg ctgatccgcc
     1441 agcgtcagtc catggcggag gtgagccggg cggtggaccg gctgtgtgtg tcgcaggggg
     1501 tggagccgcg ccacaaggcc tatccgtttt cggtgctggc ccatcgggtg ggtccgctgg
     1561 atgaacgcag ccgccgtttc agcgtggcgc gcttcggcgt acgcagtgtg gtgtggctga
     1621 gcagggcgat ggcggcgggg cagcgcaagg ggcagtcgcc gctgtggagc agtggcaagc
     1681 ggtccgacca tgcgcccatg ccgggcctgt gggcggtgga gccccacctg ggtttttacg
     1741 gcgtgggagc gaaattcgag gaactgctgg tgatcaccga tacccatgcc tggtggctgg
     1801 acgacgatct gccccatgtg cggcgctggc aggaacggca ggaatcccgg acggaaccgg
     1861 agcccgtgtt gcggttgcat gatctgacgc ggggcagtgc acaaacaagc agtgcacaaa
     1921 caagcaatgc ccaaaaaagc ggggcttcaa aaagccgtgc cgccaggagc agtaccgcat
     1981 gagcagacag cgcgaagttg tgtcgggagc ggtgcggctg gcggtgcgtt gcgacggcga
     2041 tccggccgcg ccaccggtgc tgctggtgca tggatacccc gatgccagtc aggtctggga
     2101 tgcagtggct gatcggctgg cgacggaatt cctggtgatc cgctacgacg tgcgcggcgc
     2161 cggacaatcc agcgcgcctg cggaggcgcg aggttatcgg ctggagcagt tgtcgcagga
     2221 tttacaggcg gtgatggatg cggtcagccc cgatcgtccg gtgcatctgg tggggcatga
     2281 ctggggctcg atccagacct gggaagcggt gaccgatcct gcgttgaaag aacgcattgc
     2341 atcgttcacc agtctgtcgg ggccttgcct ggatcacatg ggttactggc tgcgggacaa
     2401 attgcggcag ccttcgcctg cccatgtgct ggcgttgatg cgacagctga tccggtcctg
     2461 gtacgtgatg ctgtttcatc tgccgggcac tggcctggtg tggaaactgg gactggcgcg
     2521 gctgtggccc cggttgctgc gcatcacaga aggcatcgat gtgcccgggc ggccatcgca
     2581 atcccgtgat ggctggaacg gcgtggcgct gtaccgcgcc aattttctgc gccggctatt
     2641 gctgccgcgc gaacgtattg cagtagcgcc agtgcaactg gtgattgcgg gaaatgatgc
     2701 ctatgtcagc gcggggattt tcgacggact ggcgcgctgg gtgccggagc tgtacgtgaa
     2761 gcgcgtgcgt ggcgggcact ggctgccatt gaaacagccg acgctggtgg cgggattgat
     2821 tgctgatttt attcgtgcgc gcgaaaaccc gggtggcacg caatccaggc tgacaccggt
     2881 gcaggggccg ctgcgggaat tttccggccg gcaggtgctg gtgaccggtg ctgccagtgg
     2941 cattggtcgc gccacggcgc tcgcctttgc cgaacagggt gccagcctga tcctggcgga
     3001 tctcaatctt gacggcgcgg aacgtacggc gttgctggca cgtttgcaca gtgtgtccgc
     3061 ctgggcctgt cgcgtcgatg tgggtgacgg tgttgccatg cgcacctttg ccgacggtgt
     3121 cgaggatgca tttggcctgc ccgacatcat catcaacaac gccggcatcg gcatggccgg
     3181 cggtctgctt cacaccagcg aacgggactg ggagcgcatc ctgcgcgtga acctgtgggg
     3241 cctcattcac ggcatccgcc tgttcggcga gcgcatggtg cagtccggca acggcggaca
     3301 cattgtcaat gtggcctcgg cggcgacctt cacgccttcg cgcacgtttc ctgcttacgc
     3361 cacgacaaag gccgccgcgc tgatgctgag cgaatgcgcg cgcgccgaat tttcccgctt
     3421 cggtattggt gtgacggcgg tgtgccccgg ctttgtcgac accggcatcg cgctgtccac
     3481 ccactacacc ggcgtgaatg cggacgaaca gcaacgccgg cgcgagcagg ccgccaagct
     3541 gtatcagcgc cggggtttta cgccggaaaa agtggcggcg gccatcgtgg gtgccgtgcg
     3601 ccgcaaccgg ccattggcgc tggtgggcgt ggaagcgcag ggcgcgcgct ggatgagccg
     3661 ctttatgccc tgggccgcgc ggctgtttgc ccgcatcgat ttttcgccca actgaacaca
     3721 ggcattgaat ttaatagttt gaaaaatatg gcaggagtat tttcaccatg tctgagacac
     3781 aacatcatca tccgctggtg gcgcgcaatg tgaaattcca gtgggagaac acaccgcacc
     3841 cctggctgcc cggcgacagc tttacctcgc atttcatcaa cgtgctgcac ctgctgctgc
     3901 cggaaggcga actgtggttc tgccggctct acaatcaggc gctgccggac attgaagatg
     3961 aaaccctgcg cgccgatgtg aaaggcttta tccgccagga agcgattcac tcgcgctcac
     4021 accaggttgt gctggatcgc tattttgccg acaacggcat tgtcaccgag ccgtacaccc
     4081 gtcgtgtgcg ctggctgttc accgatctgc tgggggacca tccgttcggc atcgcgacgg
     4141 aaaggcagtg gatcaaacgc gcctggctga ccttccgcct gggcctgatt gcggccatcg
     4201 aacatttcac ctgcgtgatc gggcgctgga ttctggaatg ccggggtctg gaggaagccg
     4261 gtgccgaccc ggtgatgatg gatctgctgc gctggcatgg cgccgaagag gtggagcacc
     4321 gcacggtggc acacgatctg ttcgaacagc gcggtggtgg ctatgtttat cgtgtcatgc
     4381 tgatgttgct ggtgggccct atgttgctgc tgctatggtt cctgggtacc cgcttttttc
     4441 tgcgtcagga cccggctgcc gacccgaacc tgaaattcat ccgcgaatgg cggcgggcgg
     4501 cgcggcagga ccggttgccc accatggcat ttctgtggct gtcggtgtgg cgttatctgc
     4561 gccccggtta ccacccgcgc gatgaagcgg aaactggcca ggcactggat tatctgcaac
     4621 gttcgcctgc tgcgttggcg gctacagtac gctgattgtt ttgttctgta cctttacaca
     4681 ccggaatacc gctttcgatt cacaacaaaa aatggatgta cgacatgaaa aaaatattcg
     4741 ctggtgcatt ggcattgacc tttgtttcgc aacccatcgc tgccattcca tcgagctata
     4801 tcgatccggt atggcggcca tttctgctgc tggccggtga cggcgatccc cagcgtgcat
     4861 cggatcgtct gcagcgcatc aagcgcctgc agaccaccat gctgaacaat acccgctacg
     4921 acaccgagac tcaggcgttt gtggataatc tgtggcgttc aatggaaaaa ttcgacctgg
     4981 acggatttta cggctcgctg ccggatcaga aaggccgtca gcgggaactg aacgtgcgcg
     5041 ggatatggca ggacgatgcc tatttgtatg agcgggttcc agcggctgaa ttgagccctc
     5101 agctgaccac gttgccgctg ggct
//
LOCUS       DASUEG010000261         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1749766, whole genome shotgun sequence.
ACCESSION   DASUEG010000261 DASUEG010000000
VERSION     DASUEG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749766"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFX11_04865"
     CDS             <1..181
                     /locus_tag="VFX11_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937981.1"
                     /translation="TVSLGTFSAVPGDQCSLEQMVHGADMALYASKENGRNRVTCSSQ
                     GDWRNVEALPVHQRT"
     gene            373..2214
                     /locus_tag="VFX11_04870"
     CDS             373..2214
                     /locus_tag="VFX11_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4937982.1"
                     /translation="MLRFVLMLWVAWLCTSASASPVLPFVLQDADRVSLDTSVRYVED
                     PQGNLTADDVRRPDTAWQQNDSHAFKQGYNPSAWWLHLRIANPGSQPQARALELSYAV
                     LDYVDVYVYSGATQLQRYRMGDRYPFRQRPLDNRFFVVPLHWDAGQTLDIYYRIQTSS
                     SVQAPLALWQNDAFTSFESNSNLLQGFYYGAMVVIVVYNLLIFFMLWERAYLYYVGFV
                     LSMPLFMAALSGQGFRYLWPHSVSWNDHAIPIFLASSLMFGALFTRRFLRLGKLSNRL
                     DKMLLTFVLLGGAMLVVAPFAPYHVTIKLLVPIGLFACIADMIAGVVAWYRGVATARY
                     YCIAWGSFLICSVVLALNKMNLLPSNWFTEYSVQFGSVLEAVLLSFAMAERINVERKL
                     RYEAQSDALQVTRRLNEELEQRVQERTQELEKANRKLEALSNTDQLTGLNNRRFLEAV
                     LLEEWTRCKRYGHPLSLILLDIDFFKKVNDVHGHQVGDVCLQQVAQRVSASTRWPSDR
                     VARYGGEEFCLVLPETDADGAAIVAERIRAAVATEPVEADGKSLSVTVSLGIYTALPG
                     EECGVEQLVHCADLALYASKEKGRNRVTPFSQIDVHNVASLPVRKRT"
     gene            complement(2218..3300)
                     /locus_tag="VFX11_04875"
     CDS             complement(2218..3300)
                     /locus_tag="VFX11_04875"
                     /inference="COORDINATES: protein motif:HMM:NF044660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4937983.1"
                     /translation="MRQSYRWRMLVLGLAAIASALVAEGFEAWSTLASVNTALSGQIS
                     PNTTTISGIAPAATNASDTETQRLTQLHALHARLLAQPNRTEFQQHLLDHIQAANRFP
                     PNNRLFAATELDPVINALTPDERQSGSEDGRTRLTLRCDKRRYLPGETATIQAFQTDV
                     DSGRKIPAQLNAQLILNENRGLGPVKFADPDGDRVYTATLSSRGDPPLQQGRYKVRVS
                     TETNITDAVAFTLTDELARLTGRYRDHIGNGHLVLEAEVQSETQGSYFLRATLYADGA
                     GGGLFTQQRMELTPGRQWVALSFHGYLFQQRALDGPYRLQNITLQRISFPASTGTLSQ
                     PGYRTRAYNHREFSAHPYLELAAAPD"
     gene            complement(3513..4997)
                     /locus_tag="VFX11_04880"
     CDS             complement(3513..4997)
                     /locus_tag="VFX11_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HEX4937984.1"
                     /translation="MSELLVVVDSLKDWGPYYPTENVITFEDYLARQEENKKSRTRVI
                     NLCRNYKYLSKGYYCSLLAEARDHHVLPSVRVINDLNQRSLYTLHLDDLDEIDAKTLT
                     RGNENDTIHFRSYFGNTSNPAFKSLAKELFEKFPCPILEVELAFHRRWEIESLHALSP
                     HALEREEDETEFAESLDQFAHKIWRSPKSRKQYRYDLAILCNKDEKLPPSDKTAIKRF
                     IKAGNKLGIDVEVIDRKDYVRLAEYDALFIRETTAIDHHTFRFAKKAEAEGMVVIDDS
                     TSILRCCNKIYLTDLFNVNKVPTPKTLILAKNSKKALEQAVTDLGFPMVLKIPDGSFS
                     QGVFKVKDPEEFEQRTTELFKKSSLLLAQEYMYTDFDWRIGVLNNRPLYACRYYMAKN
                     HWQIYNHGSPSTRFTTGGYDTMPTYEVPKVVLDAALKATKLIGTSLYGVDLKQVGDRA
                     MVIEVNDNPSIESDVEDKYLDMQLYEQIMQEFVNRIEAKRGNRS"
     gene            5172..>5651
                     /locus_tag="VFX11_04885"
     CDS             5172..>5651
                     /locus_tag="VFX11_04885"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX4937985.1"
                     /translation="MRSPLLSEQTLSAFSDTYASARQQFLNTVATLPRPVFSQTLDYV
                     GSGPLGETLATDVLWLGDQDARKVVVLLSAVHGVEGFAGSAIQTDLLRRLASGEATLP
                     DGLALAIVHAVNPWGFAWCRRVDEQGIDINRNFVDFSQPLPRNDGYRALADALIPADG
                     "
BASE COUNT          985 a         1574 c         1821 g         1271 t
ORIGIN      
        1 taccgtcagt ctgggcacct tcagcgcggt gcccggcgac cagtgcagcc tggagcagat
       61 ggtgcacggt gctgacatgg cactttacgc atcgaaggaa aatggccgca accgggtgac
      121 ctgtagcagc cagggcgact ggcgcaatgt agaggcgctg ccagtacacc agcggacctg
      181 acagatcggg ggtccgttgc cagccggccg caggccgccc tttccccgga aatgaatttc
      241 tggtcacaaa ttagccgatt tttattggac gatcgtctat gctttatcaa agcaggactg
      301 ataagtctgc agcggatgca ccgatttcaa acaattgttt gataataaat aacaaaccga
      361 ggccaggttt acatgctccg ctttgttctg atgctctggg tcgcctggct ctgtaccagc
      421 gccagtgcct cccccgtgct cccgtttgtg ctgcaggatg cagatcgtgt ttccctcgac
      481 acctccgtcc gttatgtgga ggacccgcaa ggcaacctga cggccgatga tgtgcgccgg
      541 ccggacaccg cctggcagca gaacgacagc cacgccttca agcagggcta caacccgtcg
      601 gcctggtggt tgcacctgcg tatagccaat cccggatcgc agccgcaggc gcgcgcgctg
      661 gagctgtcct acgccgtgct ggattatgtc gacgtgtacg tctattccgg tgccacccag
      721 ctgcaacgtt accgcatggg ggatcgttat cccttccgcc agcgaccgct ggacaaccgc
      781 ttcttcgtgg tgccgctgca ctgggatgca ggccagaccc tcgacattta ctaccgcatc
      841 cagaccagca gttcggtgca ggcgcccctg gcactgtggc agaacgatgc cttcacctcc
      901 ttcgaaagca acagcaatct gctgcagggt ttttattacg gcgcgatggt ggtgatcgtc
      961 gtgtacaacc tgctgatctt tttcatgctg tgggagcgtg cctaccttta ttacgtggga
     1021 tttgtgctgt cgatgccgct attcatggcg gcgttgagcg ggcagggttt ccgctatctg
     1081 tggccgcact cggtgtcctg gaacgatcac gccattccta tcttcctggc gtcgtcgctc
     1141 atgttcggtg cgctgttcac ccgacgtttc ctgcgtctgg gcaagttgtc gaaccggctc
     1201 gacaaaatgc tgcttacctt tgtgctgctg ggaggtgcca tgctggtggt ggcgccgttt
     1261 gcgccctacc acgtgaccat caagttgctg gtgcccattg gcctgttcgc ctgcatcgcc
     1321 gacatgatcg ccggtgtggt ggcctggtat cggggcgtgg cgaccgcgcg ctattactgc
     1381 attgcgtggg gctcgttcct gatctgttcc gtcgtgctgg cgctgaacaa gatgaacctg
     1441 ttgcccagca actggttcac tgaatattcg gtgcagttcg gctcggtgct ggaggcggtg
     1501 ttgctgtcgt tcgcgatggc ggaacgcatc aacgtcgagc gcaagctgcg ttatgaagcg
     1561 cagtccgacg cgctgcaggt aacccgtcgc ctgaatgagg aactggagca gcgggtacag
     1621 gagcgcacgc aggaactgga aaaagccaat cgcaagctgg aggccttgag taacactgat
     1681 cagttgacgg gcctgaacaa ccggcgcttc ctggaggcgg tgctgctcga ggagtggacc
     1741 cgctgcaaac gttatggcca tccgctgtcg ctgattctgc tcgacatcga tttcttcaag
     1801 aaggtgaatg acgtgcatgg ccatcaggtt ggcgatgtgt gcctgcagca ggtggcgcag
     1861 cgtgtcagtg ccagtacccg ctggccgtcg gaccgggtgg cccgttacgg tggcgaggaa
     1921 ttctgcctgg tgctgccgga aaccgatgcc gatggtgcgg ccattgtggc agagcggatt
     1981 cgcgcggcgg tggcaacaga gcccgtcgag gccgatggca agtcgctgag cgtcactgtc
     2041 agcctgggca tctatacagc cttgcccggt gaggagtgcg gtgttgaaca gctggtgcat
     2101 tgtgccgatc tggcgctgta tgcatcgaag gaaaaaggcc gcaaccgggt gactcccttc
     2161 agccagattg atgtgcacaa tgtggcgtcg ctgccggtgc gcaagcgtac ctgaccatca
     2221 gtccggcgcg gcggccagtt ccagataagg atgggcgcta aactcccgat ggttgtaggc
     2281 cctggtgcgg taaccgggct gggacagggt gccggtggac gcgggaaatg aaatccgttg
     2341 cagcgtgatg ttctgcagcc ggtacgggcc gtccagcgcc cgttgctgga acagatagcc
     2401 gtggaacgac agcgccaccc actgccgtcc tggcgtaagc tccatgcgct gttgcgtgaa
     2461 caggccaccg ccggcaccgt ctgcatacag ggttgcccgt agaaaatagg agccttgggt
     2521 ttcgctctgt acttccgctt ccagcaccag atgtccgttg ccgatatgat cgcgatagcg
     2581 tccggtcaga cgcgccagtt cgtcagtgag ggtaaaagcc acggcgtcag tgatgttggt
     2641 ttccgttgac acccgtacct tgtaccgccc ttgctgcagt ggcggatctc cccgggagct
     2701 cagcgtggcg gtgtagaccc ggtcgccgtc gggatcggca aatttcaccg ggcccaggcc
     2761 gcggttttca ttcagaatca gctgcgcgtt cagttgcgcg ggaattttgc gcccgctgtc
     2821 cacgtcggtc tggaaggctt gaatggtcgc ggtttcaccc ggcaggtagc gacgcttgtc
     2881 gcaacgcagg gtgaggcggg tgcggccgtc ttcgctgccg gactggcgtt cgtccggcgt
     2941 cagtgcgttg atgaccggat cgagctctgt ggcggcaaat aatcggttgt tgggcggaaa
     3001 gcggttggct gcctggatgt gatcgagcag gtgctgctgg aattcggtac ggttgggttg
     3061 ggccagcagc cgggcgtgca acgcatgcag ctgggtcagg cgttgtgttt cggtatcact
     3121 cgcgttcgtt gcggctggcg caatgccgga gatggtggtc gtgttggggg agatctgccc
     3181 ggaaagcgcc gtatttacac tggccagcgt tgaccaggct tcgaagcctt ccgccaccag
     3241 tgcactggcg atggcagcca gccccagaac cagcatccgc cagcgataac tctgcctcat
     3301 gtcattgcct cgtccagccg tcccggtcga cgtttgggaa tgcagcagct ggcgcatggc
     3361 gatcattttc cggatgacgt gggaatggga gtgtcatggc catgctacgg gggcagcggc
     3421 cgtttccgga aatacgaaag tgggattgtc agaggcggag caatttgcta gcgcaccatg
     3481 gcgggcgcgc tgttgcggtc agaatcaggt ggtcaggaac ggttgccgcg cttggcttcg
     3541 atgcggttga cgaactcctg catgatctgt tcgtacagct gcatgtcgag gtatttgtcc
     3601 tccacatcgg attcgatgct ggggttgtcg ttcacttcga tcaccattgc ccggtcgccc
     3661 acctgtttca ggtcgacacc gtacaggctg gtgccgatca gcttggtggc tttcagggcg
     3721 gcatccagca ccacctttgg cacttcgtag gtgggcatgg tgtcgtagcc gccggtggtg
     3781 aagcgagtgc tggggctgcc gtggttgtag atctgccagt gattcttcgc catgtagtaa
     3841 cggcaggcgt acagcggacg gttgttgagc acgccgatgc gccagtcgaa atccgtgtac
     3901 atgtattcct gcgccagcag cagggaggat ttcttgaaca gctcggtggt gcgctgctcg
     3961 aattcctccg gatctttcac cttgaacacg ccttgcgaga aggagccgtc gggaattttc
     4021 agcaccatgg gaaagcccag atcggtcacg gcctgttcca gcgctttttt gctgttcttg
     4081 gccaggatca gggttttcgg tgtgggcacc ttgttcacgt tgaacaggtc ggtgaggtaa
     4141 atcttgttgc agcagcgcag gatggacgtg gaatcgtcga tgaccaccat gccttcggct
     4201 tcggcctttt tcgcaaagcg gaaggtgtga tggtcgatgg ccgtcgtctc gcggatgaac
     4261 agcgcatcgt attccgccag acgcacgtag tccttgcggt cgatcacttc cacgtcgatg
     4321 cccagcttgt tgccggcctt gatgaagcgc ttgatggccg tcttgtcgct ggggggcaac
     4381 ttttcgtcct tgttgcacag gatcgccaga tcgtagcggt actgcttgcg tgacttgggc
     4441 gagcgccaga tcttgtgggc gaactggtcc agcgattcgg caaattcggt ttcgtcctct
     4501 tcccgctcca gcgcgtgggg cgacagcgcg tgcagactct cgatttccca gcggcggtga
     4561 aaggccagtt ccacttccag gatcgggcag gggaattttt cgaacagctc cttggccagc
     4621 gatttgaatg ccgggttgct ggtgttgccg aaatagctgc ggaagtggat ggtgtcgttt
     4681 tcgttgccgc gggtgagggt cttggcgtcg atttcgtcga gatcgtcgag gtgcagggtg
     4741 tagagtgagc gctggttcag atcgttgatc acccgcaccg acggcagcac gtgatgatcg
     4801 cgcgcctcgg ccagcagcga acagtagtaa cccttcgaca ggtatttgta gttgcggcac
     4861 aggttgatga cgcgggtgcg cgactttttg ttttcttcct gacgggcgag gtagtcctca
     4921 aacgtgatga cgttttccgt ggggtaatag ggaccccagt ctttgagaga atctacaacg
     4981 actaatagct ctgacattag tacctgaacc gacgggaaga acgaagaagt gcactctttg
     5041 gcatgccttg gcattttgga ttcaagctcc ccggattaca aggatgaaaa aagaaaaaag
     5101 tgaaccgggt ctggcaaaca ggcggggtgg gcgctaccct agttttctta ctggcagtgc
     5161 gagtcccgcc tatgcgctcc cccctcttgt ccgagcagac cctctcagcc ttttccgaca
     5221 cttacgccag cgcccgccag cagttcctga acacggtggc tacgctgccc cgtccggtgt
     5281 tttcgcagac cctcgattac gtcggctcgg gccctctggg cgagaccctg gccacggatg
     5341 tgctgtggct gggcgatcag gacgcgcgca aggtggtggt gctgctgtcg gcggtgcacg
     5401 gggtggaggg ctttgccggt tcggcgatcc agaccgatct gctgcggcgt ctggccagcg
     5461 gcgaggcgac gctgccggac gggctggcgc tggccatcgt gcacgcggtg aatccctggg
     5521 ggtttgcctg gtgccgccgt gttgacgagc agggcatcga catcaaccgc aatttcgtgg
     5581 atttcagcca gccgctgccg cgcaacgacg gctaccgcgc gctggcggat gcgctgattc
     5641 ccgccgacgg c
//
LOCUS       DASUEG010000262         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_359837, whole genome shotgun sequence.
ACCESSION   DASUEG010000262 DASUEG010000000
VERSION     DASUEG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359837"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFX11_04890"
     CDS             <1..1059
                     /locus_tag="VFX11_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4937986.1"
                     /translation="ALMDAAVDAIIVIDHRGLIQEFNPAAERLFGYSEQDVLLRNVSV
                     LMPEPYAAEHDRYLGRYNTTGEARIIGIGREVQARRQDGSVFPIDLSVGEVKEGNLTF
                     FVGIIRDISDRKEVQELHRRLAHVTRLSTLGEMASGIAHEINQPLTAIATYAQAARRL
                     LEKGATSDLDAVLDKIAKQAERAGAVIRQMRSFVRRQDREQALSHPAELVASVVQFLQ
                     MDSGRRNIDIHQELPEDLPTLMVDTVQIQQVLLNLLLNAMDATESAGSRESVRVRAYL
                     DDHSSVLFEVLDRGCGVSEEHRGQLFTPFFTTKDAGMGMGLPICRSIVQAHGGQLNFR
                     PNPGGGSVFHFSLPALLE"
     gene            1096..1731
                     /gene="fixJ"
                     /locus_tag="VFX11_04895"
     CDS             1096..1731
                     /gene="fixJ"
                     /locus_tag="VFX11_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEX4937987.1"
                     /translation="MQETKTTSATVYVVDDDEAVRDSIQLLLQSVDYAVETFDSPLTF
                     LKAAPAIQSGCLVLDVRMPAMSGLELQQRLKQNGVPIPVIFITGHGDVPMAVQAMQAG
                     AFDFLQKPFRDQDLLDRIGEALAWDRNRSRGGRRQDSIRTNLKLLSPREREVLDRVVQ
                     GQANKVIAADLELSQRTVEIHRARVMEKMQAESLADLVRMMLIIETDPTNP"
     gene            1849..2253
                     /locus_tag="VFX11_04900"
     CDS             1849..2253
                     /locus_tag="VFX11_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4937988.1"
                     /translation="MDQSVAGHAGITTPHRIDILLAEPDEAVRDSMQLLFETVGFAVE
                     SARDAGQVEALLLQQLPRCLVMASEFTPAGGIALLSQLREHGFNVPAIITACRGGVPL
                     AVEAMRAGATDFLEKPLLDARLLLAVKKVMSP"
     gene            2344..3483
                     /locus_tag="VFX11_04905"
     CDS             2344..3483
                     /locus_tag="VFX11_04905"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX4937989.1"
                     /translation="MPKQLPEDSVGIVQPQSFRWDAPITLQCGKTLPRYDLVYETYGQ
                     LNAARSNAVLVCHALSGHHHAAGYHSENDSKPGWWDACIGPGKAIDTDRFFVVSLNNL
                     GGCHGSTGPSTQNPETGKPYGPDFPIVTVKDWVISQKALMDFLGIQQWAAVVGGSLGG
                     MQAMQWSIDFPDCVRNAVVIAAAPKLSAQNIAFNEIARKAILLDPDFHGGHFYEKNTY
                     PKTGLILARMLGHITYLSDAAMREKFGRELRDGSISFDFDVEFQVESYLNYQGEVFSK
                     NFDANTYLLMTRALDYFDPAADFNDDLAEALRRAKANFMIMSFTTDWRFSPERSEEIV
                     TALLAAKKNVSYADIDCPQGHDSFLLDVPRYISMFRAYMNAIEVD"
     gene            complement(3499..>4068)
                     /locus_tag="VFX11_04910"
     CDS             complement(3499..>4068)
                     /locus_tag="VFX11_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX4937990.1"
                     /translation="KHALNGLPRANLQHIDSILAEWQQVLGADYEGYRNHVVRMVTFC
                     LMLRRLASQDVSEEDQRKIEIAGCFHDIGLWTGNTLDYLSPSLPPARRYLAAQGLDGW
                     SSEIEQMILLHHKVTPVQDSEPALAELFRRGDLVDFSLGAVRFGLPGKVVRKVQAIFP
                     NAGFHKMLARRSARWAVQHPLNPLPMLKW"
BASE COUNT          822 a         1320 c         1203 g          723 t
ORIGIN      
        1 gccctgatgg atgcggcggt ggatgccatc atcgtgatcg accaccgcgg cctgatccag
       61 gaattcaacc cggcggcaga gcgcctgttc ggctacagcg agcaggacgt gctgctgcgt
      121 aatgtcagcg tgcttatgcc agagccctat gcggcggaac acgaccgcta cctgggccgc
      181 tacaacacca ctggcgaggc ccgcatcatc ggcatcggcc gcgaagtgca ggcccggcgc
      241 caggacggct cggtctttcc catcgacctg tcggtgggcg aagtgaagga aggcaacctc
      301 acgtttttcg ttggcatcat ccgcgacatc agcgaccgca aggaagtgca ggagctgcac
      361 cgccggctgg cccatgtcac ccgcctgagc accctggggg aaatggcatc cggcatcgcc
      421 cacgagatca accagcccct caccgccatc gccacctatg cgcaggccgc ccgccggctg
      481 ctggaaaagg gcgccacatc agatctggac gccgtgctgg acaagattgc caagcaggcg
      541 gaaagggccg gtgcggtgat ccgccagatg cgcagtttcg tgcgccggca ggaccgggaa
      601 caggcgctgt cccaccccgc cgaactggtg gccagtgtgg tgcaattcct gcagatggac
      661 tccggccgcc gcaatatcga cattcaccag gaactgcctg aagacctgcc taccctgatg
      721 gtggacaccg tgcagatcca gcaggtgctg ctgaacctgc tgctcaatgc catggacgcc
      781 accgaatccg ccggcagccg cgaaagtgtg cgggtgcggg cctacctgga cgaccattcc
      841 agcgtgctgt tcgaggtgct ggatcgcggc tgcggcgtca gcgaggaaca tcgcggccag
      901 ctgttcactc cctttttcac caccaaggac gccggtatgg gcatggggct gccaatctgt
      961 cgcagcatcg tccaggccca cggcggccag ttgaattttc gccccaatcc cgggggcggc
     1021 agcgttttcc acttctcgct gcccgccctg ctggagtgag gccttagtac ctgttgatct
     1081 gaccgggacc caaccatgca agaaacaaaa acaaccagcg ccaccgtcta tgtggtggac
     1141 gacgatgaag ccgtgcggga ttccatccag ctgctgctgc aatcggtgga ctatgcggtg
     1201 gagaccttcg attcacccct gacgtttctg aaagcagcac cggccatcca atcgggctgc
     1261 ctggtactgg atgtgcgcat gccggccatg agcgggctgg aactgcagca gcgcctgaag
     1321 cagaacggcg tgccgattcc cgtcatcttc atcaccggcc acggcgatgt gcccatggcg
     1381 gtgcaggcca tgcaagccgg ggccttcgac ttcctgcaga agcccttccg cgaccaggat
     1441 ctgctggacc gcatcggcga ggccctggcc tgggaccgca accgcagccg gggcggccgc
     1501 cgccaggaca gcatccgcac caacctgaaa ctgctgagcc cacgcgagcg ggaagtgctg
     1561 gaccgggtag tgcagggtca ggccaacaag gtcattgccg ccgacctgga actgagccag
     1621 cgcaccgtcg agatccaccg cgcccgggtc atggagaaaa tgcaggcgga atccctggcg
     1681 gacctggtgc ggatgatgct gatcatcgaa accgacccca ccaatccctg atccccgtca
     1741 acgaaggggc cgggctaggt acaagcacgt atggcccggt catccttctg acctcaaaat
     1801 agaacaagtc cgcactccat gtgcgtgttt agtaacgagg acttgttcgt ggaccagtca
     1861 gtggccggcc atgccgggat caccacccct cacaggatcg acattttgct ggcggaaccg
     1921 gatgaagccg tccgcgacag catgcagctg ctgttcgaaa ccgtgggttt tgccgtggag
     1981 tccgcccggg acgccggcca ggtggaagcc ctgctgctgc aacaactgcc gcgctgcctg
     2041 gtcatggcgt cggagttcac cccggccggc gggatcgccc tgctgtcgca gctgcgggaa
     2101 cacggcttca acgtgccggc catcattacc gcctgccggg gcggcgtgcc cctggcggtg
     2161 gaagccatgc gcgccggtgc caccgacttc ctggaaaaac ccctgctgga tgcccgtctg
     2221 ctgctggcgg taaaaaaggt gatgagcccg tagccgtcac ccttgccacc caccctttcc
     2281 acccccccca attctgatag actgcgcaca ccttgaactc ttgcccggaa gtcacctgcg
     2341 ccgatgccga aacagttgcc tgaggattcg gtgggaatcg tgcagcccca gtctttccgc
     2401 tgggacgccc ccatcaccct gcaatgcgga aaaaccctgc cccgctacga tctggtctat
     2461 gaaacctatg gccagctgaa tgctgcccgc agcaacgccg tgctggtgtg ccacgccctg
     2521 agcggccacc accatgccgc cggttaccac agcgagaacg acagcaagcc cggctggtgg
     2581 gacgcctgca tcggccctgg caaggccatc gacaccgacc gcttcttcgt cgtcagtctg
     2641 aacaacctgg gcggctgcca cggcagcacc ggccccagca cccaaaatcc cgaaaccggc
     2701 aaaccctacg gccccgactt tcccatcgtc accgtgaaag actgggtgat cagccagaaa
     2761 gcactgatgg attttcttgg catccagcaa tgggcggctg tggtgggcgg cagcctgggc
     2821 ggcatgcagg ccatgcagtg gagcatcgac ttccccgact gcgtgcgcaa tgcggtggtc
     2881 atcgccgccg cacccaaact gtcggcgcag aacatcgcct tcaacgaaat tgcccgcaag
     2941 gccatcctgc tggacccgga tttccacggc ggccatttct acgaaaaaaa cacctacccg
     3001 aaaaccggcc tgatcctggc gcgcatgctg ggccacatca cctacctgtc ggacgcggcg
     3061 atgcgggaaa aattcggccg cgaactgcgt gacggctcga tcagttttga tttcgacgtg
     3121 gaattccagg tggagagtta tctgaattac cagggcgagg tgttctcgaa aaacttcgac
     3181 gccaacacct acctgttgat gacacgggca ctggattatt tcgatccggc ggcggatttc
     3241 aacgacgatc tggcggaagc gctgcgccgg gccaaagcca atttcatgat catgtccttc
     3301 accaccgact ggcgtttctc gccggaacgg tcggaggaaa tcgtcaccgc actgctggcg
     3361 gccaaaaaga acgtgagtta cgcggatatc gattgcccgc aggggcatga ttcatttctg
     3421 ctggacgtgc cgcgctatat cagcatgttc cgggcgtaca tgaatgcaat tgaagtggat
     3481 tgaactgatt ctgcttcatc accacttcag catcggcaat ggattcagcg gatgctgcac
     3541 cgcccagcgc gcagagcgcc gggccagcat tttgtgaaag cccgcgttgg gaaaaatcgc
     3601 ctgcaccttg cgcacgacct tgccgggcag accaaagcgg acagccccca gcgagaaatc
     3661 caccagatcc ccccgccgga acaattccgc cagtgcgggc tcactgtcct gcaccggcgt
     3721 caccttgtga tgcagcagaa tcatctgctc gatttcgctg ctccatccat ccaggccctg
     3781 ggctgccaga tagcggcgcg ccggcggcag cgagggtgac agataatcca gcgtgttgcc
     3841 ggtccacaat ccgatatcat gaaagcaccc ggcaatctcg attttgcgct gatcttcttc
     3901 gctgacgtcc tgggaggcaa gacgcctcag catcaggcaa aaagtcacca tgcggacgac
     3961 atgattgcga taaccctcgt agtcggcgcc gagcacttgc tgccactcgg ccaggatgct
     4021 gtcgatatgc tgaagattgg cgcggggaag tccgtttaat gcgtgttt
//
LOCUS       DASUEG010000263         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_559202, whole genome shotgun sequence.
ACCESSION   DASUEG010000263 DASUEG010000000
VERSION     DASUEG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559202"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFX11_04915"
     CDS             complement(<1..963)
                     /locus_tag="VFX11_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX4937991.1"
                     /translation="MPLSRLLMPFVAKLATSPKLRKFRRGWFEIKRRLVPSLNTVVFY
                     HRVDDPYSYLLLQALPRFLEDFRVNLQIRFVLDLPVDGVPQPKLLERYALEDGKRLAA
                     LHELVYPEHPQAPSREDAFHASAILLKHQDRPKLLHLVTEVTGALWGCSTTTFESCLR
                     RYGTLPDRETQALLDSASADLASRGHYQSAMLYYGGEWYWGLDRLGHLADRLNKPRLR
                     RTSGDIADYQRQYRHVLQSYSTLRPRPRQVHPLDFYFSFRSPYSYLAADRVFKLAELY
                     KIPVTIKPVMPMVTRGIPLPKAKRLYIIKDAKREAEKYNLPFGKI"
     gene            complement(1008..2312)
                     /gene="argA"
                     /locus_tag="VFX11_04920"
     CDS             complement(1008..2312)
                     /gene="argA"
                     /locus_tag="VFX11_04920"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX4937992.1"
                     /translation="MHTQDYVKWFRASSPYINAHRDKTFVVAFGGEAVQHANFMHVIQ
                     DIVLLHSLGIRMVLVHGARPQIDARLQESNISTRVEQHRRVTDLAALPSVIEAIGRTR
                     IQVEAGLSQGIAAAHLQGSKTQVASGNFVTARPYGVRNGVDYQHSGEVRKVNAEAIRT
                     QLHNGALVLLSPLGFSPTGEVFNLLWEEVAEAAAVALQADKLIYLNADTGVLGDSGEL
                     IRELTVPQATDLLAKLDAPHYTLISATRALKQVGRAHLISYQTDGALLQELFTVDGSG
                     TLVTQQNFENIRNATIDDVGGVLELIEPLEQEGILVRRSRELLETEISQFTLIERDEK
                     IIACAALYPFTADRTGELACVAVHPDYRHGKRGDQLLQAIEQRARGMGLNRLFVLTTR
                     TAHWFMERGFTAATVAELPQHKQSLYNYQRNSKVFIKILDPA"
BASE COUNT          422 a          708 c          847 g          485 t
ORIGIN      
        1 gatcttgcca aagggcaggt tgtacttttc cgcctcgcgc ttggcgtcct tgataatgta
       61 caggcgcttg gccttgggca ggggaatgcc gcgcgtcacc atgggcatca ccggtttgat
      121 ggtgacggga atcttgtaca gttccgccag cttgaacacc cggtccgccg ccagatagga
      181 gtaggggctg cggaaggaaa aatagaaatc cagcggatgc acctggcgcg ggcgcgggcg
      241 cagggtgctg tagctttgca gcacgtggcg gtactggcgc tggtagtcgg cgatgtcgcc
      301 gctggtgcgg cgcaggcgtg gcttgttgag gcggtccgcc agatgaccca ggcggtcgag
      361 gccccagtac cattcgccgc cgtaatacag catggcggac tggtagtgtc cgcgggaagc
      421 cagatcggcg ctggcactgt ccagcagggc ctgggtttcg cggtcaggca gagtgccgta
      481 acgccgcagg cagctttcga aggtggtggt gctgcagccc cagagcgcgc cggtgacttc
      541 ggtgaccaga tgcagcagct tggggcggtc ctgatgcttg agcagtatcg cgctggcgtg
      601 gaaggcgtct tcccggctgg gcgcctgcgg atgctcgggg tataccagtt cgtgcagtgc
      661 cgccagccgc ttgccgtcct ctagcgcgta acgctccagc agtttgggct ggggtacacc
      721 gtccaccggc agatccagca cgaaacggat ctgcagattg acgcggaaat cttcgaggaa
      781 acgcggcaat gcctgcagca acaggtagga atagggatcg tccacccggt ggtagaacac
      841 cacggtgttg agggacggca ccagacggcg cttgatttcg aaccagccgc ggcggaactt
      901 gcgcagtttc gggctggtgg ccagtttcgc cacgaagggc atcaacagtc ggctcagtgg
      961 cataggttca gtggcatggc tggttcagac ttccctcgca gcggcggtca ggccgggtcc
     1021 agaatcttga tgaagacttt cgaattgcgc tggtaattgt agagcgactg tttgtgctgc
     1081 ggcagttctg ccaccgtcgc cgccgtgaag ccccgctcca tgaaccagtg cgccgtgcgg
     1141 gtggtgagca cgaacaaccg gttcagaccc atcccgcggg ccctttgttc aatcgcctgc
     1201 agcaactggt cgccgcgctt gccatggcga tagtctggat gcaccgccac gcaggccagt
     1261 tcgccggtgc ggtcggcggt gaacgggtac agcgcagcgc aggcaatgat tttctcgtcg
     1321 cgttcgatca gggtgaactg ggaaatttcc gtttccagca gctcacggga gcgccgcacc
     1381 aggatgcctt cctgctccag cggctcgatc agttccagca cgccgcccac atcgtcgatg
     1441 gtggcgttgc ggatgttctc gaaattctgt tgcgtgacga gggtgccgct gccgtccacg
     1501 gtgaacagtt cctgcagcag ggcaccgtcg gtctggtagc tgatgaggtg agcgcggccc
     1561 acctgtttca gggcgcgggt ggcactgatc agggtgtagt ggggtgcgtc cagcttggcc
     1621 agcagatcgg tggcctgggg taccgtcagt tcgcggatca gttcgccact gtcgcccaga
     1681 acgccggtgt cggcgttgag ataaatcagc ttgtcggcct gcaatgccac cgcggcagct
     1741 tccgccactt cttcccacag caggttgaag acttccccgg tgggggaaaa acccagcggc
     1801 gacagcagca ccagcgcgcc gttgtgcagc tgcgtgcgga tggcctcggc gttcaccttg
     1861 cgcacttcgc cgctgtgctg gtagtcgacg ccattgcgca cgccataagg ccgggcggtg
     1921 acgaaattac cggacgccac ctgggtcttg ctgccctgca gatgcgcggc cgcgatgccc
     1981 tgggacaggc cggcctcgac ctggatgcgg gtgcggccga tggcttcgat tacggacggc
     2041 agcgccgcca gatcggtcac gcggcgatgc tgttccaccc gggtgctgat attgctttcc
     2101 tgcaggcgtg cgtcaatctg cgggcgggca ccgtgcacca gtaccatccg gatgcccagg
     2161 ctgtgcagca gcacgatgtc ctgtatcacg tgcatgaagt tggcatgctg cacggcttcg
     2221 ccgccgaacg cgacgacgaa ggttttgtcg cggtgagcgt tgatgtaggg cgaactggcg
     2281 cgaaaccatt tgacgtaatc ctgcgtgtgc atgggtgggg acagcgactc ctgggccggg
     2341 tgaacgggat tgcaactgca taagctacag ggaaatttcc gtgtttggca aacgtcttgc
     2401 cgcctttcca gtgtggcaca ggcaagcggg agtgtcagtg ggcgcggcgg gcagggttgt
     2461 gg
//
LOCUS       DASUEG010000264         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1596685, whole genome shotgun sequence.
ACCESSION   DASUEG010000264 DASUEG010000000
VERSION     DASUEG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596685"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1027
                     /locus_tag="VFX11_04925"
     CDS             446..1027
                     /locus_tag="VFX11_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HEX4937993.1"
                     /translation="MPSKIRSLSLLPSLIGVTALALSGASQAHTLDLGINNDAVALDY
                     SGQLPKSELNLGGGFLHHKDNGDAYYASLFVADSVNKESGLLAGLGARFYFLDADEGS
                     TDGKSLALGGFLNWDLPSVPNLSLRGDLYYAPDVLSFDDIENFADVSARVQYRVIEQA
                     WVYLGYRRLMLEDELSRDFNLEEGGFVGMLIWF"
     gene            1180..2238
                     /locus_tag="VFX11_04930"
     CDS             1180..2238
                     /locus_tag="VFX11_04930"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX4937994.1"
                     /translation="MLALNCGMTALLPAASLAQPSGGVVASGDGTITSGPVTSVQQNS
                     EHLALNWGSFNIQPGEQVIFVQPGTSSVALNRDFSGIPSELFGSLQANGQVFLLNTAG
                     VLIGPSATINVGGLLVSDLSLSDDEFARLQAENQMTLTETNPATGGIVNQGSVTSSTR
                     NGITLIGQYIDNAGTLTANNGNINLAIADGPIVVTDSSGMLGVQLSQGLGQNLAPSGA
                     LLNNSGEIRAVQGNIAINIRYQSGLNVQAVNNTGLVNAVGIGYGNIDQNIVLEPAPVD
                     PGDQSDAVDIISDSFVKETDDSSKGLAGDAPRKPATNVDELISSCNEDENDSNECKKQ
                     NAIKRYLGRLLIGGSLPD"
     gene            complement(2241..>2856)
                     /locus_tag="VFX11_04935"
     CDS             complement(2241..>2856)
                     /locus_tag="VFX11_04935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4937995.1"
                     /translation="EHNNFSIRAHKLNDDEIGDAIDAFNAMLGKIEQDKEELTRLAYY
                     DPLTRLANRRMFSERLVFALENARRSGEQMGLIFLDLDKFKVINDELGHDIGDLLLKA
                     AAKRLETAIPESATAFRLGGDEFTVLQVGATEKSMTETAEKILQAFGEPLILAGRQLS
                     ISSSIGIAISDGHDNVSSIMKSADVALYRAKDAGRSNYKFFHQA"
BASE COUNT          632 a          817 c          779 g          628 t
ORIGIN      
        1 cctatggggc ggcgcgttct gcgcgccgca ggaagcgggc gaaccgattt gggtgttctt
       61 gggagcgtgg caaaatcgtg aatcatattc cctatgacag actgacaggc aaattgatca
      121 tatcaaccgg gtaactggct aaactataga ccatatatcc aatattgatc attatttgtg
      181 aaaaattata agcttacaag gcgaagttga gccctgaaaa caggtaattt tgctaattgc
      241 agcataggcc gcacgtttac cggacacacc ctcgccccct cacaccgttt ccgggagctg
      301 cctagcccgg caactttgcc ggatagtcct gtctaaccgt ttgaaaatcg ctggattttg
      361 ggctccgcct cggattgaac ggcgaccgaa agcaactgat aatgacccga tcctcttgta
      421 taactccctt tccaggacct tgaacatgcc ttctaaaatc cgctccctca gccttttacc
      481 ttctctgatc ggtgtaaccg cgctggccct ctcgggcgca agccaggcac acacgctgga
      541 cctgggcatc aacaacgacg ccgttgcact tgattacagc ggccaactgc ccaaatccga
      601 actgaacctg ggtggcggct tcctgcacca caaggacaat ggcgatgcgt actacgccag
      661 cctgttcgtg gcggacagcg tcaacaagga atccggattg ctggcgggcc ttggcgcccg
      721 cttctatttc ctcgacgccg acgaaggcag cacggatggc aagagcctgg cgctgggtgg
      781 tttcctgaac tgggacctgc catcggtacc taacctcagc ctgcggggtg acctttatta
      841 cgcccctgac gttttgtcat tcgatgacat cgagaatttc gccgacgttt ccgcccgcgt
      901 gcagtatcgg gtgatcgagc aggcgtgggt gtatctgggt tatcgccgcc tgatgctgga
      961 agacgaactg tcgcgggatt tcaacctcga agaaggcggt ttcgttggca tgctgatctg
     1021 gttctgacgc aagggcaatg cctgacggtc tggccgggcc ggtgctaagc ttcttttacc
     1081 agtcgttaag aggtggtgga tgatggcacg cggctcggag cagacccggt gacaaaaaga
     1141 caggctttct cgaccgtttg gggcaggctg ccaggttgca tgcttgcgct caactgcggc
     1201 atgacagctc tgcttcctgc cgcctctctg gcgcagccca gcggtggagt ggttgcgagc
     1261 ggtgacggca ctatcacgtc gggccccgtg acgtcagtgc agcaaaacag cgagcatctg
     1321 gccctcaact ggggctcctt caacatccag ccaggggaac aggtgatatt cgttcaaccc
     1381 ggcacatcct ccgtggcact gaaccgcgat ttttccggta ttcccagcga actgttcggc
     1441 agcctccagg ccaatggcca ggtattcctc ctgaataccg ctggtgtgct gatcggaccc
     1501 tcagccacca tcaatgttgg cggtcttctg gtatcggatc tgtcgctgag cgacgatgaa
     1561 tttgcccgcc tccaggccga aaaccagatg accctgactg aaaccaatcc cgcaaccggc
     1621 ggaatcgtca accagggctc agtgacatcc agtacccgca acggcataac actgattggt
     1681 cagtacatcg ataatgccgg taccctcacc gccaacaacg gcaacatcaa tcttgccatt
     1741 gcggatggcc ccatcgtcgt aacggattcg tcgggcatgc tcggtgtgca actcagccag
     1801 ggactgggcc aaaacctggc tcccagcggt gcattgctga ataacagcgg tgagatccgt
     1861 gcggtgcagg gcaatattgc catcaatatc cggtaccaga gtggattaaa tgtgcaggct
     1921 gtcaacaaca ccggactggt gaacgccgtg ggtatcggat atgggaacat cgaccagaat
     1981 attgtgctgg agcctgcccc agtcgatccc ggtgatcaga gcgatgcggt cgacattatt
     2041 tccgattcct tcgtgaaaga aacggacgac agctcgaaag gccttgcagg tgacgcgccg
     2101 cgcaaaccgg cgaccaacgt cgatgaattg atctccagct gcaatgagga cgagaacgat
     2161 agcaacgaat gcaagaaaca gaatgcgatc aaacgctatc tgggacggtt gctgatcgga
     2221 ggcagcctgc cggactgagt tcaggcctgg tgaaagaatt tgtagttgct gcggccggca
     2281 tccttggccc ggtagagcgc gacatccgca ctcttcatga tcgaactgac attgtcatga
     2341 ccatcggaaa tggcaatgcc gatactggac gaaatcgaca gctgtctccc ggccagaatc
     2401 aggggctcac caaatgcctg caatattttc tcggctgttt ccgtcatcga cttttccgtt
     2461 gcccctacct gcagcacggt gaattcatca ccacccaggc ggaatgcggt ggcagattcc
     2521 ggaatggccg tttccagccg cttggccgcc gctttcagta acagatcgcc tatgtcgtgg
     2581 ccaagctcat cgttgatgac tttgaactta tccagatcca gaaaaatcag ccccatctgc
     2641 tcaccactgc ggcgcgcatt ttccagcgca aacaccagcc gttcgctgaa catgcggcgg
     2701 ttggccagcc gggtcagcgg atcatagtaa gccagccgcg taagctcttc cttgtcctgt
     2761 tcgatcttgc ccagcatggc attgaaagcg tcaatcgcgt cgccgatttc gtcatcattc
     2821 agcttgtgtg cgcgaatgga aaaattgttg tgctcg
//
LOCUS       DASUEG010000265         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_474208, whole genome shotgun sequence.
ACCESSION   DASUEG010000265 DASUEG010000000
VERSION     DASUEG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474208"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFX11_04940"
     CDS             <1..423
                     /locus_tag="VFX11_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX4937996.1"
                     /translation="MLPVATSANTVSQHLDSHTLSGAFVLGGVLGLIWSPCSGPLLAS
                     TLTLVASEGGATQGALILGIFGAGAATPLVAVAYASRQGFAHARDWVLAHVESMKKGF
                     GLVLGLVGIAILTGADKWLETQLLTVLPDAWVQLTVKF"
     gene            complement(430..1809)
                     /gene="cysG"
                     /locus_tag="VFX11_04945"
     CDS             complement(430..1809)
                     /gene="cysG"
                     /locus_tag="VFX11_04945"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX4937997.1"
                     /translation="MDYLPIFLNIREQRCLVVGGGEVATRKAAMLIRAGARVHVVAPK
                     ISPALVEKLLTHECSYRLGGYDASDLEDVAIVIAATDDEALNRRVHSDCRTRQLPVNV
                     VDAPALCTFVFPSIVDRSPLIIAVSSAGKSPVLARLLRARLETTIPAAYGRLADICGE
                     FRPLAKQTFPDTNNRRGFWERELQGRFAELVYAGREDEARAHLREAFASARPQQAGEV
                     YLVGAGPGDPDLLTFRALRLMQQADVVVHDRLVSEPILDLCRRDADFIHVGKARADHT
                     MPQEDINQLLVRLAKEGKRVCRLKGGDPFIFGRGGEEIEELAAAGIPFQVVPGITAAN
                     GCSAYAGIPLTHRDYAQSVRFVTGHLKDGSPELPWDELIHEQQTLVLYMGLVGLDHIC
                     QQLMAHGMAADMPVALVSQGTRPDQMVVTGTISDIAEKVTESGIKAPTLTIVGRVVEL
                     RDKLNWFNT"
     gene            complement(1832..3109)
                     /gene="serS"
                     /locus_tag="VFX11_04950"
     CDS             complement(1832..3109)
                     /gene="serS"
                     /locus_tag="VFX11_04950"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX4937998.1"
                     /translation="MLDPKLLRNELDFVAERLALRNMTLDTAAIRALEEKRRNLQTET
                     EKLQSERNAGSKKIGEAKKKGEDTTAIMAEMNAIGERLKNVQVELETLQAEFEQVQLT
                     LPNLPHESVPAGKGEDDNVELRRVGTIRELGFTPKDHADLGEALGLLDFETAVKIAGS
                     RFSVLKGPLARLQRALTQFMLDTHTQEHGYEEVYVPYLANADSLRGTGQLPKFEEDLF
                     KTGGEKPYYLIPTAEVPVTNIVRDEIVPAEKMPLRFVAHTPCFRSEAGSYGKDTRGLI
                     RQHQFEKIELVQIVKPEDSHKVLEELTGHAEKILQKLELPYRVIVLCTGDMGFSSAKT
                     YDIEVWLPGQGKYREISSCSNFEGFQARRLQARYRNPETGKPELVHTLNGSGLAVGRT
                     LVAILENYQNEDGSVTIPEVLRGYMGGLEKISL"
     gene            complement(3142..3525)
                     /gene="crcB"
                     /locus_tag="VFX11_04955"
     CDS             complement(3142..3525)
                     /gene="crcB"
                     /locus_tag="VFX11_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX4937999.1"
                     /translation="MNALHPLLLVAIGGALGSMARYGAAAAVNRMAQSAFPWGTLVVN
                     VAGSFLIGLLMVLLFKAGEWRENGRLLLVTGMMGGFTTFSSFSWETWKLLEDGRLMMA
                     ITNVFASVAICLLATLAGALLARQF"
     gene            complement(3522..>4476)
                     /locus_tag="VFX11_04960"
     CDS             complement(3522..>4476)
                     /locus_tag="VFX11_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX4938000.1"
                     /translation="DGTVLFIGATTENPSFELNNALLSRARVYVLRSLEEQDLLTLVQ
                     QALRDEKRGFGKRGLKADEAALRQIARAADGDARRALNILEIAADLAENSHISDTVIA
                     ESLLGNARRFDKQGEYFYDQISALHKSVRGSDPDGALYWLARMIDGGCDPLYVARRVV
                     RMASEDIGNADPRALPLCLNAWDVQERLGSPEGELAIAQAVAYLAVAPKSNAVYKAWN
                     TVKKRVQETGSLEVPLHLRNAPTKLMKEQGYGAEYHYAHDFPDAYVAGESYLPEALAN
                     ERFYEPVPRGLEIKIGDKLGLLREKDNASQWQRVPRKRGRA"
BASE COUNT          910 a         1352 c         1269 g          945 t
ORIGIN      
        1 atgctgccgg tggcgacatc agccaataca gtgtcgcagc atctggacag tcacacactg
       61 agcggtgcat ttgtgctggg cggcgttctg ggattgatat ggagtccctg ctcgggacca
      121 ttgttggcgt cgacgttaac actggttgcc agtgagggtg gcgctacaca aggagcattg
      181 atactgggca tattcggtgc aggtgcggcg acgccactgg tcgcggttgc ctatgcgtcc
      241 cgccagggct ttgcgcatgc ccgggattgg gtgttggcgc acgtcgagtc gatgaaaaaa
      301 ggattcggtc tggtactggg cttggtggga attgccattc tcactggagc tgacaagtgg
      361 ttggagacac aactgctgac agtactgccg gacgcatggg tgcagctgac cgtgaaattc
      421 tgaacaggat tacgtgttga accagttcag tttgtcacga agttcaacaa cgcgcccaac
      481 aatggtcagc gtaggtgcct tgatgccgct ttcagtcacc ttttccgcaa tatccgaaat
      541 ggtgccggtt accaccatct ggtcggggcg cgtcccctgt gacaccagtg ccacgggcat
      601 gtcagccgcc atgccgtgag ccatgagctg ctggcagatg tggtccagcc caaccaggcc
      661 catatacagc acgagcgtct gttgctcgtg aatgagctcg tcccaaggca gttccggcga
      721 accgtccttg agatggccgg tgacaaagcg caccgattgt gcgtaatccc gatgcgtcaa
      781 cggaatgccg gcataggccg aacagccgtt agcggccgta atgccgggga ctacctgaaa
      841 cggaatgccg gcagccgcca gctcttcgat ttcttcacca ccacgaccga agatgaacgg
      901 gtcgccacct ttcaggcggc aaacccgttt gccctccttc gccagccgca ccagcaactg
      961 gttgatgtct tcctgcggca tggtgtgatc ggcacgggct ttgccaacat gaatgaagtc
     1021 ggcatcgcga cggcacagat ccaggatggg ttcggacacc aggcgatcat gcaccacgac
     1081 atcggcctgt tgcatgagtc gcagggcacg gaaggtcagc aggtcaggat cgccggggcc
     1141 ggcgcccacc agatatacct cacctgcctg ctgtggacgg gcagacgcaa acgcttcgcg
     1201 cagatgcgcc ctcgcctcgt cttcacgacc ggcatagacc agttcggcaa aacggccctg
     1261 taattccctc tcccagaagc cgcgacgatt gttggtgtcc ggaaacgttt gcttggccaa
     1321 gggacggaat tcgccgcaga tatcagccag acggccatag gcggcaggaa tggttgtttc
     1381 cagtcgtgcg cgcaacaggc gcgccagcac tggcgacttg cccgcacttg atacagcgat
     1441 gatcagcggt gaacggtcga caatggatgg aaacacaaat gtgcacagcg ctggcgcatc
     1501 caccacgttc acgggcagct gccgcgtgcg acagtccgaa tgcacccgtc gattcagtgc
     1561 ttcatcatca gtggccgcaa tgacaatggc cacatcctcc agatcagagg cgtcatatcc
     1621 accaaggcga tagctgcatt catgcgtcag cagcttttcg accagtgccg gcgaaatttt
     1681 cggtgccacc acatgcacgc gggcgcccgc gcgaatcagc atggccgcct tgcgcgtggc
     1741 cacctcccca ccccccacga ccagacagcg ctgctcacgg atattcagga atatggggag
     1801 gtagtccatg tcttgtgacc tgttcagtat gtcagagaga gattttctcg aggccgccca
     1861 tgtagccacg cagcacttcg gggatggtca cactgccgtc ttcattctgg tagttttcca
     1921 gaatggcgac cagcgtacgg cctacggcaa ggccggagcc attcagcgtg tgtaccagtt
     1981 caggcttgcc ggtttccggg ttacggtaac gggcctgaag gcgacgtgcc tggaaacctt
     2041 cgaagttcga gcacgacgag atttcacggt acttgccctg ccccggcagc cagacttcga
     2101 tgtcgtaggt ttttgccgag gaaaagccca tgtcgccggt gcacagcacg atcacacggt
     2161 acggaagctc cagcttctgc aggattttct cggcatgccc ggtcagctct tccagcacct
     2221 tgtgggagtc ttccggcttc acgatttgca cgagttcgat cttttcaaac tggtgctgac
     2281 ggatcaggcc gcgcgtgtct ttgccgtaag aaccggcttc ggaacggaag cacggcgtgt
     2341 gtgccacaaa gcgcagtggc attttttctg ccggaacgat ttcgtcacgc acaatgttgg
     2401 taacggggac ttctgctgtc ggaatcaggt agtacggctt ttcaccaccg gtcttgaaca
     2461 ggtcttcttc gaacttcggc agctgacccg taccgcgcaa gctgtcggca tttgccagat
     2521 acggcacgta cacctcttcg tagccgtgtt cctgagtatg cgtgtccagc atgaactggg
     2581 tcagcgcacg ttgcagtctg gccagcgggc ctttcagcac ggagaagcgg ctgccggcaa
     2641 tcttcacagc ggtttcgaag tccagcaggc ccagcgcttc gcccagatcg gcatgatctt
     2701 ttggggtaaa gccgagttcg cgaatggtgc cgacacgacg cagctcgacg ttgtcatcct
     2761 cacccttgcc agctggaacg gattcatgcg gcaggttcgg cagggtcagc tggacctgtt
     2821 caaattcagc ctgcaaggtc tccagttcca cctgcacatt cttcagacgc tcaccaatag
     2881 cgttcatctc agccatgatg gcagtggtgt cttcaccctt tttcttggct tcgccgatct
     2941 tcttggagcc agcgttgcgt tcggactgca gcttctcggt ttcggtctgc aggttgcggc
     3001 gcttttcttc cagcgcacga atggccgcag tatccagcgt catgttgcgc agcgccaagc
     3061 gctcggccac aaagtccagt tcgttacgca gcagtttcgg gtccagcatg atcagtcttc
     3121 ttgttagtca aaagtgaaaa atcagaattg tctggccagt agtgcaccgg ccagggtggc
     3181 cagcaggcag atcgccacac tggcaaaaac attagtgatg gccatcatca gtcggccatc
     3241 ttccagcagc ttccaggttt cccagctgaa gcttgaaaat gtcgtaaagc cgcccatcat
     3301 gccagtcacc agcagcaggc gtccgttttc acgccactcc ccggccttga acagcagcac
     3361 catcagcagt ccgatgagaa aactgcctgc tacgtttaca accagcgttc cccagggaaa
     3421 cgcggattgt gccatccggt tgactgcggc cgccgcgcca tagcgtgcca tcgagcccag
     3481 tgcaccacca atggccacca gcagcaaggg atgcagagcg ttcatgcgcg cccccttttg
     3541 cgcggaacgc gttgccactg gctagcgttg tctttttcgc gcagcaagcc cagcttgtcg
     3601 ccgatcttta tttccagacc gcgcggcacc ggctcataaa aacgctcgtt agcgagtgct
     3661 tcgggcagat aggactcccc tgccacatag gcgtccggga agtcatgggc ataatgatat
     3721 tccgcaccat acccctgctc tttcatgagc ttggtcggtg cattgcgcag atgcagggga
     3781 acttccagtg acccggtttc ctgcacgcgt tttttcacgg tattccaggc cttgtagacg
     3841 gcattactct ttggcgctac cgcgagataa gccacagcct gggcgatggc cagctcgccc
     3901 tccggcgacc ccaaacgttc ctgcacatcc caggcattca ggcacaacgg caaggcccgc
     3961 ggatcggcgt tgccgatgtc ttcgctcgcc atgcgtacca cgcgacgcgc cacatagagc
     4021 gggtcacaac cgccatcaat catgcgtgcc agccagtaca gcgcgccatc cggatcagaa
     4081 ccgcgcacgg atttgtgcag ggccgaaatc tggtcataaa aatactcacc ctgcttgtcg
     4141 aaacggcggg cattgcccaa caggctttcg gcaatcacgg tgtcgctgat gtggctgttc
     4201 tccgccagat ctgcagcaat ctccagaatg ttcaaggcac gacgggcatc gccatcggcg
     4261 gcacgcgcaa tctgacgcag ggcagcttcg tcagccttca gaccccgctt gccaaagcct
     4321 cgcttctcgt cacggagcgc ctgctgtacc agcgtcagca ggtcctgctc ttccagactg
     4381 cgcagcacat agacgcgcgc gcgcgacagc aaggcattgt tcagttcgaa ggacgggttt
     4441 tccgtagtgg cgccaataaa cagcaccgta ccgtct
//
LOCUS       DASUEG010000266         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1882030, whole genome shotgun sequence.
ACCESSION   DASUEG010000266 DASUEG010000000
VERSION     DASUEG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882030"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFX11_04965"
     CDS             <1..1294
                     /locus_tag="VFX11_04965"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX4938001.1"
                     /translation="ADLGKTLKAADRIVGVGQDKDEIQDVVGWRLDEVVDRIRGKKGT
                     VVRLEVIPADAKSDHETRIVHITRDTVRLEDRAAQSEVMDIDVNGHKERLGIISLPTF
                     YADFRCRKSGDGSCRSTTFDVKKLIEKLQTQKIDGLVIDLRNNGGGSLEEVNNLLGLF
                     IETGPTVQIRGSNGSVEVMADQDPQVLYNGPLAVVVNRLSASASEIFAGAIQDYQRGL
                     IVGERTFGKGTVQSLQELDRGQLKITMAKFYRVSGESNQHKGIIPDIEYPSLINHDEI
                     GESSLPHALPSDNIRPARYRRQHDIDTVLTYLRTAHDQRAAKDPEFQYVQDQVTAMDA
                     IRQKTLVSLNWEQRQKENHELEQQRLSIENKRRGALGEPLLKSASELKVQDEEEEDLA
                     AETTGKDKIEVDYILKETGKILTDFITIATGQTQVAVH"
     gene            1294..1716
                     /locus_tag="VFX11_04970"
     CDS             1294..1716
                     /locus_tag="VFX11_04970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX4938002.1"
                     /translation="MAFVLDSRLQRDTFLLGSNDQELLLLMNDRRYPWFIIVPTQDGA
                     SEWFDLPESAQNTLHRHCVQLGAAIHTQFHCEKVNIAALGNIVRQMHVHIVGRHTDDP
                     AWPGPVWGHSAATPYDPPALEQMKAQLLKMKELPFTLQ"
     gene            1775..>2770
                     /locus_tag="VFX11_04975"
     CDS             1775..>2770
                     /locus_tag="VFX11_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4938003.1"
                     /translation="MERHPVLQPFRLSQSACRLAFLLLGLLLVRSVTADTVAIHDREG
                     FYNLSPHLEILQDETHQLRISDLLKPDARHAWFTSNETVPSFGYANKVFWFRVKVLNE
                     HESKAEWLLNIDYPMHDFVDVYYVNHNQIIKAYHAGDRLPHSHRPIDHRNFVFPVMIP
                     VDSALDIYIRLESEGTIKMPLYLWEEARFIKTDQQFMLGQGIYVGAMAVMFFYNLFLF
                     FLVRNRAYLMYILYIFFQGSAFAISRGFGFQYIWPNWTTWNQMSVVFFLGGTITFAML
                     FSIYFLESRRYTPRYYYAMSMGACYGLAVMLGSFALEYKYMVKIGMYGLLLAMALN"
BASE COUNT          632 a          793 c          770 g          575 t
ORIGIN      
        1 tgccgatctg ggcaagacac tgaaagcagc ggaccgtatc gtgggcgtgg gccaggacaa
       61 ggacgaaatc caggacgtgg tgggctggcg tctggatgaa gtggtggatc gtatccgggg
      121 caagaaaggc acggtggtac ggctcgaagt gattcccgcc gatgcaaaga gcgatcatga
      181 aacgcggatc gtgcacatca cccgcgacac ggtaagactg gaagatcgcg cggcgcagtc
      241 tgaagtaatg gacattgatg tgaatggcca caaggaacgg ctcggcatca tcagcctgcc
      301 cacgttttat gccgattttc gctgccgcaa atccggtgac ggcagctgcc gttccaccac
      361 ctttgatgta aaaaagctga ttgaaaaact gcagacgcag aagatcgacg gcctagtgat
      421 cgacctgcgc aacaacggcg gcggttcgct ggaggaagtg aacaatctgc tgggactgtt
      481 tatcgaaacc ggcccgaccg tgcagattcg cggctccaat ggcagcgtgg aagttatggc
      541 ggaccaggat ccgcaggtgc tgtacaacgg cccgctggca gtggtggtga accgcctgag
      601 cgcatcggcc tcggaaattt ttgccggtgc cattcaggac taccagcgcg gcctgatcgt
      661 gggcgagcgc actttcggca agggcaccgt gcagtcgcta caggaactgg accgcggcca
      721 gctcaagatc accatggcga aattctaccg ggtgtccggt gagagcaatc agcacaaggg
      781 catcattccg gacatcgaat atcccagcct gatcaaccat gatgaaatcg gcgaaagctc
      841 gctgccccat gcattgccat cagacaacat ccgtccggcc cgctatcgca ggcagcatga
      901 catcgacacg gtactcacct atctgcgcac cgcgcatgac cagcgtgcag cgaaggatcc
      961 ggaattccag tatgtgcagg atcaggtgac cgcaatggat gccatccggc agaagaccct
     1021 ggtttccctc aactgggagc agcgccagaa agaaaatcac gagctggaac agcagcgcct
     1081 ctccatcgag aacaagcgtc gcggcgccct gggcgagccc ttgctgaaat cggcgagtga
     1141 gctgaaggtg caggatgagg aggaagagga tctggcggcg gaaacaaccg gcaaggataa
     1201 aatcgaggtg gactacattc tcaaggaaac cggcaagatt ctgacagatt tcattaccat
     1261 cgccactggc cagacccagg tagcggtcca ctgatggcct ttgtgctgga ttcacgtctg
     1321 caacgggata ccttcctgct gggcagcaac gatcaggagc tgctgttgtt gatgaacgac
     1381 cgccgctatc cctggtttat catcgtgccc acgcaggacg gagccagtga atggtttgac
     1441 ctgcccgaat ccgcccagaa taccctgcac cgccactgcg tgcagctggg cgccgccatt
     1501 cacacacagt ttcattgcga gaaagtcaac atcgctgccc tgggcaatat cgtacggcag
     1561 atgcatgtgc acattgtcgg ccgccatacg gacgatccgg cgtggcccgg ccccgtctgg
     1621 ggacactctg ccgcaacccc ctacgacccg ccggcacttg aacaaatgaa agcgcagttg
     1681 ctgaaaatga aggagttgcc ctttacactg caataagccg tggtcacgat tcactcatta
     1741 tttagcgact caagggatgt gagacaacac caacatggaa cgccatccag tccttcagcc
     1801 ttttcgtcta tcgcagtctg cctgccggct ggcgttcctg ctgctgggcc tgttactggt
     1861 ccgttccgtc acggccgaca ctgtggccat ccatgatcgg gaaggctttt acaacctttc
     1921 accccacctg gaaatcctgc aggacgaaac acatcagttg cgcatcagcg acctgctgaa
     1981 acctgacgcc cgccacgcct ggttcaccag caatgaaacc gttcccagct ttggctacgc
     2041 caacaaggtt ttctggtttc gggtgaaggt gctgaacgaa cacgaaagca aggcggagtg
     2101 gctgctgaat atcgactatc cgatgcacga tttcgtcgac gtttactatg tcaatcacaa
     2161 ccagatcatc aaggcatacc acgcagggga tcggctgccc cactcccacc gccccatcga
     2221 tcatcgcaat ttcgttttcc ccgtcatgat cccggtggat tccgcactgg atatttatat
     2281 ccgcctggaa tcggaaggca ctatcaagat gccgctgtac ctgtgggaag aagcgcgttt
     2341 catcaagacc gaccagcagt tcatgctggg ccagggcatt tacgtgggcg ccatggcggt
     2401 aatgtttttc tataacctgt tcctgttttt cctggtgcgc aaccgggctt acctgatgta
     2461 catcctgtac atcttttttc agggcagtgc ctttgccatc tcacggggtt tcggcttcca
     2521 gtacatatgg ccgaactgga ccacctggaa ccagatgtct gtcgtatttt tcctgggcgg
     2581 caccatcacc tttgccatgc tgttctcgat ttatttcctc gaaagccgcc gctacacccc
     2641 ccgctactac tacgcgatgt ccatgggtgc ctgctatggg ctggcggtga tgctgggaag
     2701 cttcgctctg gaatacaagt acatggtaaa gatcggcatg tatggtctgc tgctggccat
     2761 ggcattgaac
//
LOCUS       DASUEG010000267         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2201579, whole genome shotgun sequence.
ACCESSION   DASUEG010000267 DASUEG010000000
VERSION     DASUEG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201579"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFX11_04980"
     CDS             complement(<1..963)
                     /locus_tag="VFX11_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4938004.1"
                     /translation="MPHGFFLELLILLASSLAVLALCYPLRIPPILGYILVGVLIGPG
                     GLGWISRPESVVQIAEFGVVFLLFSLGLEFALPKMMAMRRLVLGIGAGQVVITLLALW
                     GVAWAMGVPLATGWILAGALALSSTAVVTRELTQNHQLHLRFGQIAVAVLLFQDMASV
                     FFLAILPSLGGQPEQLLPSLLWTLGKATVLLAGLLILGPRLLPRLFTLVARTRTEELF
                     VLAVLVVILAAAALTQLMGLSMALGAFIAGMLLGESHFRHQIEAEIRPFRDVLLGIFF
                     VAVGMLIHLGPLVAQLHWIILLTASFMVLKWIVVVVLARLLGETP"
     gene            complement(1035..1655)
                     /locus_tag="VFX11_04985"
     CDS             complement(1035..1655)
                     /locus_tag="VFX11_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX4938005.1"
                     /translation="MAGAADAYKEGVQYRLVESPQRITPGDKVEVAEFFSYGCHVCYD
                     IEPHLESWIKANAAQVDFVRVTHGPDFFRRSWGAYGQAYYALETLGAADKINAPLFKA
                     IHQDKERLDSESRMADFVARQGIDKKAFTAAYDSFAVRTKLKQAQAQWERFGILLLGT
                     PAFVVNGRYIVSPGFQQQVTDRTAKLTETLKVIDYLVAKERKSPAN"
     gene            complement(1746..>2010)
                     /locus_tag="VFX11_04990"
     CDS             complement(1746..>2010)
                     /locus_tag="VFX11_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4938006.1"
                     /translation="KLYRGGNKVSGVSACMACHGPSGAGNGPAGYPHLSGQHAEYVKL
                     QLEAYRRGERGNGTPSGQIMQSIAKRMSNPEIDAVSAYIDGLH"
BASE COUNT          437 a          623 c          605 g          345 t
ORIGIN      
        1 gggggtttcg cccagcaggc gcgccaatac caccaccacg atccatttca gcaccatgaa
       61 gctggcggtg agcaggatga tccaatgcaa ctgggccacc aacggcccca ggtggatcaa
      121 cattcccacc gccacgaaga aaatgcccag cagcacgtcg cggaacggcc ggatttcagc
      181 ctcgatctga tgacgaaaat ggctttcgcc caacagcatg ccggcgatga aggcccccag
      241 cgccatcgac aaccccatca gttgggtcag ggccgccgcc gccaggatca ccaccagcac
      301 cgccagcacg aacagctctt cggtgcgggt gcgtgccacc aaggtaaaca gtcggggcag
      361 cagccgcggg ccgagaatca acagcccagc cagcagcacg gtcgccttgc ccagggtcca
      421 cagcagggag ggcagcaact gctcaggctg cccacccagg gaaggcagga tggcgaggaa
      481 gaacaccgat gccatatcct gaaacaagag aacggccacg gcgatctggc cgaagcgcag
      541 atggagctgg tggttttgcg tcagttcccg ggtgaccact gccgtcgagg acagggccag
      601 cgcgccggcg agaatccagc ccgttgccag gggcacaccc atggcccagg ccacacccca
      661 caacgccaat agggtgatca ccacctgtcc ggccccgatg cccagcacca gccgtcgcat
      721 tgccatcatc ttgggcaggg cgaattcgag gcccaaggaa aacaacaaaa acaccacgcc
      781 gaattcggcg atctggacca ccgattcggg tcgggagatc catcccagtc cacccggacc
      841 gatcagcacg ccgaccagga tatagccgag gatgggggga atccgcagtg gatagcacag
      901 cgccagcacg gccagcgagc tggccagcag gatcagcagt tcaaggaaga acccatgggg
      961 catggcggcg aaccgaccgg aggatcagcg gagtttccgt gactatacca tccggccggc
     1021 ttgcagcctg agggtcagtt tgcggggctt ttgcgttcct tggcgacgag gtaatcgatc
     1081 accttcaggg tttccgtcaa cttcgcggtg cggtcggtga cctgctgctg aaaacctggg
     1141 gaaacaatgt atctgccgtt caccacaaag gccggcgtgc ccagcagaag aatgccgaat
     1201 cgctcccatt gggcttgcgc ctgcttgagc ttggtgcgta cggcaaagga gtcgtaggct
     1261 gccgtgaaag cctttttatc gatgccttgg cgggcaacga agtcggccat gcgtgactcg
     1321 ctgtcgagcc gttccttgtc ttggtgaatt gccttgaaca gcggtgcgtt tattttatcg
     1381 gcggcgccca gcgtttcgag ggcgtagtag gcctgtccat aggcccccca gctgcggcgg
     1441 aaaaaatccg ggccatgggt gacccgaacg aaatcgactt gggctgcgtt ggccttgatc
     1501 cacgattcaa gatgaggctc gatgtcatag cagacatgac agccgtagga gaagaactcg
     1561 gccacctcaa ccttgtcgcc gggggtgatg cgctggggac tttccaccaa tcggtactgc
     1621 acaccttcct tgtaggcgtc tgccgcgccg gccaagccca tccagcccaa caggcaacag
     1681 atcagcaaag cgcgtgaaaa tgatcgtatc cgatgtccca tgatgaaaaa tctctctgtg
     1741 gatgcttagt gcagtccatc aatataggcg gataccgcat cgatttccgg gttgctcatc
     1801 cgcttggcga tactctgcat gatctgaccg ctgggcgtgc cattgccccg ttccccgcgg
     1861 cggtaggctt ccaactgcag cttgacgtat tcggcatgct ggcccgatag atgggggtaa
     1921 cccgccggcc cgttgcccgc gccacttggg ccatggcagg ccatgcaggc ggagacaccg
     1981 gacaccttgt tgccgccacg gtagagcttg
//
LOCUS       DASUEG010000268         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_733377, whole genome shotgun sequence.
ACCESSION   DASUEG010000268 DASUEG010000000
VERSION     DASUEG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733377"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="aspS"
                     /locus_tag="VFX11_04995"
     CDS             <1..1284
                     /gene="aspS"
                     /locus_tag="VFX11_04995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX4938007.1"
                     /translation="TPILTRATPEGARDYLVPSRTHQGSFFALPQSPQLFKQLLMVAG
                     MDKYYQIAKCFRDEDLRADRQPEFTQIDIEASFINEEDIMGVAEGMARKLFKDLLGIE
                     FGPFPRMPYSEAMSRFGSDKPDLRIPLELVDVGDLMKQVDFKVFSGPAHDPKGRVVAL
                     KVPGGATISRKEIDDYTKFISIYGAKGLAWIKVNELAKGIDGLQSPIVKNMTNIVMEM
                     MQRLEAKDGDIIFFGADKAKVVNESMGALRIKLGHDLKLLTREWAPLWVVDFPMFEET
                     DNGGWTPCHHPFTAPSCPVEMLESDPGAALSRAYDMVLNGTELGGGSIRIHRPEMQKA
                     VFRALKISDDEANEKFGFLLDALKFGCPPHGGLAFGLDRLIMLMTGASSIRDVIAFPK
                     TQTAACVMTAAPSPVDPKQLREVGIRLREETKPKE"
     gene            1289..2041
                     /locus_tag="VFX11_05000"
     CDS             1289..2041
                     /locus_tag="VFX11_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4938008.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRSKIFTKFIREITVAARTGGGVID
                     DNPRLRLVVDKALTANMSRDIIERAIKRGVGEDDAAQMEELRYEGYGIGGVAVLVEVM
                     TDNRNRSASDVRHAFSKCGGHLGTDGSVAYLFSKRGEIFLEAGTDENRVIEVALEAGA
                     DDILTQSDGSIDVITPWETLGKIRMALESAGIVVAHADICMAPSLQVEMDEDTAARNL
                     KLIDMLEDLDDVQAVFTNAHFTVELMEKIGGG"
     gene            complement(2227..2751)
                     /locus_tag="VFX11_05005"
                     /pseudo
     CDS             complement(2227..2751)
                     /locus_tag="VFX11_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
     gene            3077..3259
                     /locus_tag="VFX11_05010"
     CDS             3077..3259
                     /locus_tag="VFX11_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938009.1"
                     /translation="MKTLNAIVFGLIAALAGTLIVFARSADLLPMLADHSVIVIMAIG
                     ILGLVGAREKTPNPQA"
     gene            3407..3931
                     /gene="ruvC"
                     /locus_tag="VFX11_05015"
     CDS             3407..3931
                     /gene="ruvC"
                     /locus_tag="VFX11_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX4938010.1"
                     /translation="MAIVLGIDPGSRITGYGVISAEGSRLHYIDSGCIRTESATDLSE
                     KLDMIFRGIDEIIRLHQPAQVGVEQVFMARNADSALKLGQARGAAIVACTTHQLPVSE
                     YSARQVKQSVVGTGAAEKTQVQRMVCHLLGLEKSPQADAADALAVAICHSHMSQSMLR
                     LAGARKVRRGRLRL"
     gene            3958..4575
                     /gene="ruvA"
                     /locus_tag="VFX11_05020"
     CDS             3958..4575
                     /gene="ruvA"
                     /locus_tag="VFX11_05020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX4938011.1"
                     /translation="MIGRIHGTLLEKQAPRLLVDVGGVGYEIEVPLTAYYRLPESGQA
                     ITLHTHFVVREDAQLLYGFLDKAERELFRVLIKVNGVGPKMALAILSGIEGADFVRCV
                     QNHDINTLIKLPGVGRKTAERLLVEMQDRLKDFSHWLPSLPATMGAGDTKRTARIEIS
                     EAESALIALGYKPQEAARAIAVLNTEGLKSEDIIRQALKNMVKAS"
     gene            4643..>4852
                     /locus_tag="VFX11_05025"
     CDS             4643..>4852
                     /locus_tag="VFX11_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX4938012.1"
                     /translation="MLESDRLISPQSGKEDAHDRAIRPTSLADYTGQNAVREQMEIFI
                     AAARGRSEALDHTLIFGPPGLGKTTL"
BASE COUNT         1018 a         1470 c         1444 g          920 t
ORIGIN      
        1 accccgatcc tcacccgtgc cacgccggaa ggtgcccgcg attatctggt gccaagccgt
       61 acgcaccagg gcagcttctt cgcgctgccg caatcacccc agctgttcaa gcagctgctg
      121 atggtggcgg gcatggacaa gtattaccag atcgcgaagt gcttccgcga cgaagacctg
      181 cgcgccgacc gccagcctga attcacccag atcgatatcg aagcttcctt tataaatgag
      241 gaagacatca tgggcgtggc cgaaggcatg gcgcgcaagc tgttcaagga tctgctgggt
      301 atcgagttcg gcccgttccc gcgcatgccg tactcggaag ccatgtcccg tttcggcagc
      361 gacaagcccg acctgcgcat tccgctggag ctggtggacg tgggcgatct gatgaaacag
      421 gtggatttca aggtgttcag cggcccggcc catgatccga aaggccgagt ggtagcgctg
      481 aaagtgcccg gtggcgccac catcagccgc aaggaaatcg acgactacac caaattcatc
      541 tccatttatg gcgcgaaggg tctggcctgg atcaaggtca acgagctggc gaaaggtatc
      601 gacggcctgc aatcgcccat cgtgaagaac atgaccaata tcgtcatgga gatgatgcag
      661 cgcctggaag cgaaggacgg cgacatcatt ttcttcggcg cagacaaggc caaagtagtg
      721 aacgagtcca tgggcgcact gcgcatcaag ctcggccacg acctgaaatt gctcacccgc
      781 gagtgggcgc cgctgtgggt ggtggatttc cccatgtttg aggaaaccga caacggcggc
      841 tggactccat gtcaccatcc gttcaccgcg ccgtcctgcc cggtggaaat gctggaatcc
      901 gatccaggtg ccgccctgtc ccgcgcctat gacatggtgc tgaacggtac cgagctgggt
      961 ggcggttcca tccgtatcca ccgtccggaa atgcagaagg ccgtgttccg cgccctgaaa
     1021 atctccgacg acgaagccaa cgagaaattc ggcttcctgc tggatgcgct gaaattcggt
     1081 tgcccgccgc acggtggtct ggcctttggt ctggatcgtc tgatcatgct gatgacgggt
     1141 gcttcctcca tccgcgacgt gattgcattc cccaaaaccc agactgccgc ctgtgtaatg
     1201 acagctgcgc cgtcgccggt ggacccgaaa cagttgcggg aagtgggtat ccgcctgcgg
     1261 gaagaaacca aacccaagga gtaatccgat ggcgggccat tccaaatggg ccaacacgcg
     1321 ccaccgcaag gcggcgcagg acgccaaacg cagcaagatc ttcaccaagt tcatccgcga
     1381 aatcaccgtc gccgcccgta ccggcggcgg tgtcatcgac gacaatcccc gcctgcggct
     1441 ggtggtggac aaggccctca ccgccaacat gtcccgcgac atcatcgaac gcgccatcaa
     1501 gcgtggcgtg ggtgaagacg atgccgcgca gatggaagaa ctccgctacg aaggttacgg
     1561 catcggtggc gttgccgttc tggtcgaggt gatgaccgac aaccgcaacc gctcggccag
     1621 cgatgtgcgc cacgccttca gcaaatgcgg tggccatctg ggcaccgatg gctctgtcgc
     1681 ctacctgttc agcaagcgcg gtgaaatatt cctggaagcc ggcaccgacg aaaaccgggt
     1741 aatagaggtg gcgctggagg cgggggcgga cgacattctc acccagtcag acggcagcat
     1801 cgacgtcatc actccctggg aaaccctcgg caaaatccgc atggcgctcg aatcggctgg
     1861 tattgtggtg gctcatgctg acatctgcat ggcgccctcg ctgcaggtgg agatggatga
     1921 agacaccgcg gcccgtaatc ttaagctgat cgacatgctg gaggatctgg acgatgtgca
     1981 ggccgtcttc accaacgccc acttcacggt ggagctgatg gaaaaaatcg gcggcggttg
     2041 aagccggaat ttctgcggcg acaaaaccct acccaataaa aagccctgtc agcgattgct
     2101 ggcagggctt tctttatatt gcaggcggta tcaggaacgc ggggcggttg ccggatactg
     2161 ggtggtggtc ggatattgcg aagccggctg atagttcggg ccagcggccg cagcagaggc
     2221 ggggggctga tccagcacca ggcggccatc cttcaccgga atctcggtgc ccggatcgta
     2281 tgccatgcgc actacgtctt cccgctcctt caggcggtag gtgacgtcat agcccactac
     2341 atcctcgcgt gattcgtagg tggtcttgca gcgctgctcg gtggtggtgt aggtgtcgtt
     2401 tttctgcatg ttgttctgca cccggtcgcc ggcatagccg ccggccacgg cgccgaccac
     2461 tgtcgccgct ttcttgccat tgccgccgcc cacctgatgg ccgagcgcac cgccaatcac
     2521 tgcgccaatc gccttgccgg ctatccggtg ctggtcctgt actggccgct ggcgggtcac
     2581 ggttacgtca cggcattctt catggggaat ctgggtggtc tgggtgaccg gtactgcgcg
     2641 cagaacaacg gcatgatccg gctccttcag caactggtag cccgaaatcg cgccgcctgc
     2701 ggttgccaca cccacgccca acagggtacc tgcgatcata gatttgttca tggtcaacac
     2761 tgctcctgat tgttgatggt cttgaaagtc ttgaagctac tgtctggtac tggaataaac
     2821 gtcggcagtt taaacatttt caggctgaat gaaccctgaa tgtatgcagg atgtgaagag
     2881 gatctcgaag tagtggcagt ccggtgcggt catgcggggc acgcattggt gcaagcgtgc
     2941 caacttggcc cggttttgcg tccggcagag tgtccacttt gcgccggtac tatttcataa
     3001 cgccaaaaat agtgcatacg tagactgtgc ccattgataa cgcagccatg atggcgcgag
     3061 caccaggggc actgacatga aaacacttaa cgcaatcgta ttcggactta tcgctgctct
     3121 ggccggcacg ctgattgtat tcgcccgttc ggcggatctg ctgcccatgc tggcggatca
     3181 ctccgtaatc gtcatcatgg ccatcggcat actcggactc gtcggtgctc gcgagaaaac
     3241 gcctaacccc caggcgtaaa acctggcggc accttcgggt gccgcttttt ttgcccccgc
     3301 ccgaaatccc acctacagcc ttatgaaatt agctcagaaa taaagtgaca gctgcgcttg
     3361 cgctgctaga ctagcttcca aatcaaacag ttggaatgga acaggcatgg cgatcgtgct
     3421 cggcatcgat ccaggctcgc gcatcaccgg ttacggcgtc atcagcgccg aaggctcacg
     3481 gctccactac atcgacagcg gctgcatccg cactgaatcc gccaccgatc tcagcgaaaa
     3541 gctcgacatg atttttcgag gcatcgacga gataatccgc cttcaccagc cggcccaggt
     3601 gggcgtcgaa caggtgttca tggcccgcaa cgccgattcc gcgctcaagc tggggcaggc
     3661 gaggggggcc gccatcgtcg cctgcactac gcatcagctg cccgtttccg aatattctgc
     3721 ccgccaggtg aaacagtcgg tggtgggcac tggcgcggcg gaaaagaccc aggtgcagcg
     3781 catggtgtgt catctgctcg ggctggaaaa atcgccccag gccgatgcag cggatgccct
     3841 ggcggtggcc atctgccatt cccacatgtc ccaaagcatg ctgcggctcg ccggcgcccg
     3901 caaagtgcgg cgcggacggc tgcggctctg actgcagaga cttggacagg agagcccatg
     3961 atcggacgta ttcacggaac ccttctggaa aaacaggccc cccgactgct ggtggatgtg
     4021 ggtggcgtcg gctacgaaat cgaagtgccg ctgaccgctt actacagatt gccggaatcc
     4081 ggccaggcga tcaccctgca cacacacttc gtggtgcgcg aagacgccca gctgctgtat
     4141 ggctttctcg acaaggccga gcgggaactg ttccgcgtgc tgatcaaggt gaatggcgtg
     4201 gggccaaaga tggcgctggc catcctgtcc ggcatcgaag gcgccgactt tgtccgctgc
     4261 gtgcagaatc acgatatcaa cacactcatc aagctgccgg gcgtggggcg caagaccgcc
     4321 gagcgattgc tggtggaaat gcaggatcgc ctcaaggact tcagccactg gctgccgtcc
     4381 ctgccggcca caatgggtgc cggcgacaca aaacgcacgg cgcgcatcga aatttcagaa
     4441 gcggaaagtg ccctgattgc gctaggatat aagccccagg aggcagcccg cgccatcgcg
     4501 gttctgaaca cggaaggtct caagagcgaa gacatcatca gacaggcgct caaaaacatg
     4561 gtgaaggcgt cctgacctga aagcgccggc atcgaacacg cacaacgaat tacgcaaacc
     4621 agagccccag accccgacca tcatgctcga atccgatcgc ctcatttccc ctcagtccgg
     4681 caaggaagat gcccatgacc gggccatccg tcccacgagt ctggcggatt acaccggcca
     4741 gaatgccgtg cgcgagcaga tggaaatctt cattgcggcc gcccgtggcc gcagcgaggc
     4801 gctggaccac accctgattt ttggcccgcc cggcctgggc aagaccacgc tg
//
LOCUS       DASUEG010000269         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2133427, whole genome shotgun sequence.
ACCESSION   DASUEG010000269 DASUEG010000000
VERSION     DASUEG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133427"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFX11_05030"
     CDS             <1..1119
                     /locus_tag="VFX11_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4938013.1"
                     /translation="LLSGLGVQHNALMQRNMRFRERARIATTAVAISGTIALGAALLG
                     AGPWALVAQAITSELITLCLLWRASDFRPGAFRWSRDAAEMLRFGGQFLLFRVLGYAA
                     HNLQVVLVGREVGVAAAALYTRAYFFNRQILGYANDTAGQLANSALPKLIENPVRLRQ
                     FYLRCLNVIFLATAPVAVACGLHGADIVSFLFGPQWLAAGKSLEILSVGMALQPLLFS
                     TGWLYFAYGDMAGLVRWGMFGWVVMIAAGIVGVRWGVQGVAWAWSAGIVLLALPCCHY
                     AYQGRSIRLQDVLPIVSRPLGAALLAGAASSAASAALSLPSVYARLPVDACIMAATYF
                     GLLWWPFGQRPLIDEIIQSLRRASPPHTSTLSSSPPHV"
     gene            1112..1960
                     /locus_tag="VFX11_05035"
     CDS             1112..1960
                     /locus_tag="VFX11_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX4938014.1"
                     /translation="MSDAYRSRLGEKINTLANLVRPWIAQVPGLHRSFRRLRARLPEA
                     QGSPTAALMAAFGRAYPKAYFIQVGANDGAIYDPLRRQIVARHWHGIMIEPVPHIFAK
                     LAKNYRDEPRVSLENIAIGAVDGQATFHSLAPVEDPIAAGLPRWYSGLGSFRLDVILS
                     HRKHIPDIEQRVVTQTLPCLTFDSLCQKHQVQQLDLLQIDTEGYDYEVLKLVDFPRLH
                     PRLIIYEHVHLSTDDRSAAESLVRGQGYELAHHRLDTLALRTAGLDRDHPGLLKAWRS
                     HFPTAA"
BASE COUNT          347 a          690 c          584 g          406 t
ORIGIN      
        1 ctgctctcag gcctgggggt acaacacaac gccctgatgc agcgaaacat gcgctttcgc
       61 gaaagagcgc gcatcgcgac tacggcagtc gccatctcag gcacgatcgc gctgggggcc
      121 gcactactgg gcgccggccc ctgggccttg gtcgcacaag ccatcaccag cgaattgata
      181 acgttgtgcc tgctttggcg ggcgtcagat tttcgccccg gcgcgttccg ctggagccgt
      241 gacgccgcag agatgctcag gttcggcgga cagttcctgc tcttccgggt gctcggttat
      301 gccgcccaca atctacaagt cgtgctggtc gggcgcgagg tcggggtcgc ggcagccgcc
      361 ttgtatacac gcgcctattt cttcaaccgt cagatcctgg gatacgcgaa cgacacggcc
      421 ggccagctcg ccaacagtgc gcttcccaag ctgattgaaa atccggtacg gctgcgccag
      481 ttctatttgc gctgcctgaa tgtcattttc ctggcaaccg cacctgttgc cgttgcctgc
      541 ggacttcatg gcgccgacat tgtttcattc ctgttcggtc cacagtggct tgcggcgggc
      601 aagagcctgg agattctcag cgtgggcatg gccctgcagc ccctgctgtt cagcaccggc
      661 tggctgtact tcgcttacgg ggacatggcc gggctggtgc gctggggcat gttcggctgg
      721 gtggtgatga tcgcggccgg gatcgtgggc gtccgttggg gcgtacaggg cgttgcgtgg
      781 gcctggtccg ccggcatcgt gctgctggcc cttccgtgct gccactatgc ttaccaaggc
      841 cgctcgatca ggctgcaaga tgtcctgcct atcgttagtc gccccctggg tgccgccctg
      901 ctggccggcg cggccagtag cgcggccagt gcggcgctat cccttcccag cgtgtacgcg
      961 cgacttcccg tagacgcttg catcatggcc gctacgtatt ttgggctgct gtggtggccg
     1021 tttgggcaga gacctctgat cgacgaaatc atccagagcc tgcgccgggc atcgccccca
     1081 cacacctcca cactgtcctc ttcccctcca catgtctgac gcctatcgca gccgccttgg
     1141 cgagaagatc aacacgctcg caaacctcgt gcgcccctgg atcgcacaag ttccaggttt
     1201 gcaccgttcg tttcgccgcc tccgggcacg gctcccggaa gcacagggca gtcctacagc
     1261 cgcccttatg gcggcgttcg gacgcgccta ccccaaggct tacttcatcc aggtaggcgc
     1321 caatgatggc gcgatctacg accccctgcg tcgccagatc gtggcgcggc actggcacgg
     1381 catcatgatc gaacccgtgc cacatatctt cgccaagctt gcgaaaaact atcgggatga
     1441 accacgggtt tcgctggaaa acatcgccat cggagccgtc gatggacagg ccacgttcca
     1501 ctcccttgca ccggtggaag atccgatcgc cgccggcctt ccacgctggt actccgggct
     1561 aggctcattc cggcttgatg tcatcctttc acatcgcaag cacataccgg atatcgaaca
     1621 acgcgtagtg acacagaccc tgccctgcct gacgttcgac tccctctgcc aaaagcatca
     1681 ggtccagcag ctggacctgc tgcagatcga cactgaaggg tacgactacg aggtcctcaa
     1741 gctggtggac tttccccgct tgcatcccag gctcatcatc tacgagcacg tgcatctgag
     1801 caccgatgat cgatccgccg cagaatccct ggtgcgcggc caaggatatg aacttgcaca
     1861 ccaccgcctg gacacactgg ccctgcgaac cgcaggcctg gaccgcgacc acccaggcct
     1921 gctgaaggcg tggcgctccc acttccccac cgcagcctag attccgttca tgagccgccc
     1981 gccgatcgca ctggtcgtga cctcgatcca gccgcccaac gccatca
//
LOCUS       DASUEG010000270         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_799676, whole genome shotgun sequence.
ACCESSION   DASUEG010000270 DASUEG010000000
VERSION     DASUEG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799676"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="VFX11_05040"
     CDS             complement(<1..2267)
                     /locus_tag="VFX11_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4938015.1"
                     /translation="MKFSSVPGGPAIRQVALILLAALAGCDGGGSGDPYQPPALSDGV
                     VFTYPFSGQTDVPTGTRFYVGFSRNASQSAVDASCSVDGGGTVSGNFCLVGPGNRLVA
                     ISPTVNGKVVEFETDQLLQGTQYALHVRSAVIGGGDTNLPSSGALITFTTSQNDPVSG
                     AVPAVRAINGEQPAVYLPGSIQTGRYPFMDFATVRVEFTEPLDEKTVQVGSSFQFLQV
                     EGATETPVVGSLMVRRQHVSFDPDEDLIPGATYRVRLTSAVRDLNGEALADVTYELVP
                     EDANACDCVITQHFNTTVAFGEAGFPATSFTTGHALNSIDLYSPLIGANDINLRDSTV
                     QAELADPGNFQGLIPFVIRRGNFLNITGLDLRLGGEVPANLQTGDIKATFLSDVTGFL
                     GRNPYRSADTIPDDTRSPVSVYLIFDLALTSTDSAGNAVLNQTIPHVQATGTARIVNG
                     NLTIETVRTLTMDLLGLDLAPAHMVLGINSDLSASKPDDTTAPTITGSYPADGATDFP
                     IRDGLSLIFSEAIDNAGLVANDQISLIDLSNGNALVPFQLTFDGSTVLLKPDAALTFG
                     HQYRITLGALTDISDNHNPLVLDGTDATGGDGMIQFTTENPSASGAVGPMVSSINGGA
                     GCTLTGSSYASPGHCTGGLGGDTNYQPFDMPADGYLDVQFNQAMNTATLVAGSSCNSG
                     RVRVEILDGSGNCTGVVAGSLMADTRSFRFKPTQPWVAGTDYRLTLMGGGNATCDSAT
                     EVCGSNNLPLNPDPL"
     gene            2492..>2884
                     /locus_tag="VFX11_05045"
     CDS             2492..>2884
                     /locus_tag="VFX11_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938016.1"
                     /translation="MKHRLLTALIVGTGLLLSACTNTIKYDTKPLKPVVKTNVNMPEF
                     QKATQSMVSAMLQAPRVLEITGNQRPRLAVFGLVDFTGEDIDITALNNGMFAQIGQAA
                     KFSFADPAALTKADQAQSPNRYDLLEDPA"
BASE COUNT          554 a          940 c          870 g          520 t
ORIGIN      
        1 ttgagcggat cgggattcag cggcagattg ttgctgccgc acacttcagt cgccgagtcg
       61 caggtcgcat tgccgccgcc catcagggtc aggcggtaat cggtgcccgc cacccagggc
      121 tgggtcggct tgaagcggaa cgaccgggta tcggccatca gcgatcccgc caccacaccg
      181 gtgcagttgc cgctgccatc caggatttcc acccgcaccc ggccgctgtt gcaactgctg
      241 cctgccacca gggtggcggt gttcatcgcc tggttgaact gcacatccag ataaccgtcc
      301 gccggcatat cgaacggctg gtagttggtg tcaccaccca atccacccgt acagtggccc
      361 gggctggcgt aactgctgcc ggtcagcgtg cagccggcac cgccgttgat cgaactcacc
      421 atcggcccca ccgcgccgct ggccgaggga ttttccgtgg tgaactggat catgccgtca
      481 ccgccggtgg cgtcggtgcc atccagtacc agtggattgt ggttgtcgct gatgtcggtg
      541 agcgcgccca gcgtgatgcg gtactggtgg ccaaacgtca acgccgcatc cggcttcagc
      601 agcacggtgc tgccgtcaaa ggtgagctgg aacggcacca gggcattgcc gttgctcaga
      661 tcgatcaggc tgatctggtc attcgccacc agcccggcgt tgtcgatcgc ctcactgaaa
      721 atcagcgaca ggccatcgcg gatcgggaaa tcagtggcgc catctgccgg atagctgccg
      781 gtaatggtgg gcgctgtcgt gtcatcgggc ttgctcgcac tcagatccga attgatgccc
      841 agcaccatat gcgccggcgc cagatccagc cccagcagat ccatggtcag ggtgcgcacg
      901 gtttcaattg tcagattgcc gttcacgatc cgggcggtgc cggtggcctg cacatgggga
      961 atggtctggt tcagcaccgc attgcccgcg ctgtcggtac tggtcagggc cagatcgaaa
     1021 atcaggtaca ccgacaccgg cgacctggtg tcatcgggaa tggtatcggc gctgcggtag
     1081 ggattgcggc cgaggaaacc ggtgacatcc gacaggaaag tcgccttgat atcgccagtc
     1141 tgcagattgg ccggcacctc gcctcctaac cgcagatcca ggccggtgat gttgaggaaa
     1201 ttgccccgcc ggatcacgaa cggaatcagg ccctggaaat tgcccgggtc cgccagttcc
     1261 gcctgcacgg tgctgtcccg caggttgatg tcgttggcgc cgatcagggg ggaatacagg
     1321 tcgatgctgt tgagtgcgtg cccggtggtg aaagacgtgg ccgggaatcc cgcctcgccg
     1381 aatgccaccg tggtattgaa gtgctgggtg atcacacagt cgcaggcgtt cgcatcctcg
     1441 ggcaccagtt cgtaggtgac atccgccagg gcttcaccgt tgagatcccg cactgcactg
     1501 gtcagacgca cccggtacgt ggcacccggg atcagatcct cgtccggatc gaagctcacg
     1561 tgctggcgcc gcaccatcag cgaacccacc acaggcgtct cggtggctcc ctccacctgc
     1621 aggaactgga agctgctgcc aacctgcacg gttttttcgt ccagcggctc ggtgaactcc
     1681 acccgcacgg tggcaaaatc cataaacgga taacggccgg tctggatgct gcctggcagg
     1741 tacaccgccg gctgctcgcc attgatcgcc cgcaccgccg gcacggcgcc gctgaccgga
     1801 tcattctggc tggtggtaaa ggtgatcagg gcgccactgg acggcaggtt ggtgtcgcca
     1861 cccccaatca ccgcgctgcg cacgtgcagc gcatactggg tgccctgcag cagctggtcg
     1921 gtctcgaact ccaccacctt gccattcacc gtggggctga ttgccaccag tctgttgccc
     1981 gggcccacca ggcagaagtt gccgctgacc gtgccgccgc catccacgct gcagctggca
     2041 tccaccgcgc tttggctggc attgcgactg aacccgacgt agaaccgggt gccggtgggc
     2101 acatcggtct ggccggaaaa gggataggta aagaccacac cgtcggacaa ggcgggtggc
     2161 tgatagggat cgccggagcc gcccccgtca cagcccgcca gggcagccag cagaatcaag
     2221 gcaacctgcc gtattgccgg gccaccggga acagaactga atttcatgca aacctcgtga
     2281 taaccgtcgc gtcgtcaacc ctgaccccgt caccactccc ggcgcttttc cctccatttc
     2341 ctgccctaaa tatggctcac agatcgaaga ctgaccagtc acatttgagc gaatatcccc
     2401 gttccggggg ccgcacaccc ttgaaccgcc gccggattcg ggggaaactg gcgcccctgt
     2461 agccaaaaca agaacaacaa gaggggtttg catgaaacac aggttattga ccgcgctgat
     2521 agtgggaacc gggttgctgc tgtcggcctg caccaacacc atcaaatacg acaccaagcc
     2581 gctcaagccc gtggtgaaga ccaacgtcaa catgccggaa ttccagaagg ccacccagtc
     2641 catggtcagc gccatgctgc aggcaccccg ggtgctcgag atcactggca accagcgtcc
     2701 caggctggcc gtgttcggac tggtggattt caccggtgag gacatcgaca ttacggccct
     2761 gaacaacggc atgtttgccc agattggcca ggcggccaaa ttcagcttcg ccgacccagc
     2821 cgccctgacc aaggccgacc aggcccagtc ccccaaccgt tacgacctgc tggaagaccc
     2881 cgcc
//
LOCUS       DASUEG010000271         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1604905, whole genome shotgun sequence.
ACCESSION   DASUEG010000271 DASUEG010000000
VERSION     DASUEG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604905"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VFX11_05050"
     CDS             <1..1938
                     /locus_tag="VFX11_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938017.1"
                     /translation="YPESTFFGDREQFINLVFDEDQAAINAAIDTSIEENATIDVEFR
                     NKGQDGGLRWLRLQGKMLQQPNQKLHMIGVIQNITERKQTEKSLHQASMVFEATQDGI
                     LILDAQGRVLTCNSSFSLITGYAAEDMAGELPFLLSRDVLRRDQPATLQQLLNEGGQW
                     RGEIKITCKNGTSIPTLVSIVTVYDNHDKKTHFILVLTDLTAVRSAEQKLQYLAHHDP
                     LTDLPNRLLTFERLNQALLRGKRHEERVAVFFIDLDNFKWINDSLGHTIGDKLLKAIS
                     ERMIGCLRADDTIGRLGGDEFLIVLDPVESVQSVALVAQKLLRVVSQPLQLDNHVVEV
                     SCSIGISMFPDDGQYGEDLVRAADTAMYAAKEIGRNNCEFFTPAMMEKAQRYLALNHD
                     LHRGFQAGELLLYYQPQVSLRTGEITTMEVLLRWQHPKRGLLPASEIIPIAEDCGMIV
                     DIGTWVLRESCTQLARWREAGIPKARLAVNVSALQIHKSNFIDIVKEVYDACQIDPGT
                     CEIEITESTLQHEENCIDVLQQLKAMGVQITIDDFGTGYSCLSSLKLLPITKLKIDKS
                     FVNDLVTDENDTAIVQAIIAMAHKLNLEVVAEGVETREQERKLRENGCDIAQGYLYFR
                     PMSVEQVNALLAGKAPGLPLV"
BASE COUNT          478 a          598 c          608 g          432 t
ORIGIN      
        1 tacccggaga gcactttttt tggtgaccgc gaacagttca taaatctggt gttcgacgag
       61 gaccaggctg ccatcaacgc tgccatcgat accagcattg aagagaacgc caccatcgac
      121 gtggaatttc gcaacaaagg ccaggacggt ggcctgcgct ggttacggtt acagggcaag
      181 atgttgcagc agcccaacca aaagctgcac atgattggcg tgatccagaa cattaccgaa
      241 cgcaagcaga cggagaaaag cctgcatcag gcctccatgg tatttgaagc cacgcaggac
      301 ggcatcctga tcctggatgc ccagggccgt gtactcacct gcaacagcag cttttcgctg
      361 atcaccggct atgccgccga ggacatggcc ggggaattgc cgtttctgct cagtcgcgat
      421 gttctgcggc gcgaccagcc tgccacgttg caacagttgc tgaatgaagg tggccagtgg
      481 cgcggtgaaa tcaagatcac gtgcaagaat ggtacgtcca ttcccaccct ggtgagcatc
      541 gtcaccgttt atgacaatca cgacaagaaa acccatttca tcctggtgct gactgatctg
      601 accgctgttc gcagtgcgga acagaagttg caataccttg cccaccatga cccgttgacc
      661 gacctgccca accgcctgct gacgttcgag cgcctgaatc aggcgctgct gcgcggcaag
      721 cggcacgagg aacgggtggc ggtgttcttc atcgatctgg acaacttcaa gtggatcaac
      781 gattcgctgg gccacaccat cggcgacaag ctgctcaagg ctatttcgga gcgcatgatc
      841 ggttgcctgc gcgcggatga caccatcggc cggctgggtg gcgatgaatt cctgattgtg
      901 cttgatccgg tggaaagcgt gcagagcgtg gcgctggtgg cgcagaagct gctgagagtg
      961 gtcagccagc ccctgcagct ggacaatcac gtggtggaag tgtcctgcag cattggcatc
     1021 agcatgtttc ccgacgatgg ccaatatggc gaagatctgg ttcgtgccgc cgataccgcc
     1081 atgtatgccg ccaaggaaat cgggcgcaac aactgcgaat tctttacacc ggccatgatg
     1141 gaaaaagcgc agcgctacct ggccctgaac catgacctgc atcgcggctt ccaggccggt
     1201 gaattgctgc tctattacca gcctcaggtg tcattgcgta ccggcgagat caccaccatg
     1261 gaagtattgc tgcgctggca gcatcccaaa cggggcttgc tgccggcatc ggaaatcata
     1321 cccatcgcag aagactgcgg catgatcgtg gatatcggaa cctgggtgct gcgcgagagc
     1381 tgcacgcaac tggcgcgctg gcgggaggcc ggcattccca aggcgcggct ggcggtgaat
     1441 gtctctgcgc tgcagattca caaaagcaat tttatcgata tcgtaaaaga ggtctatgac
     1501 gcctgccaga ttgatcccgg cacctgtgaa attgaaatta ccgaaagcac cctgcagcat
     1561 gaggagaact gcatcgatgt gctgcagcag ctcaaagcca tgggcgtgca gattaccatt
     1621 gatgatttcg gcaccggtta ttcctgcctg agctcgctca agcttctgcc catcaccaag
     1681 ctgaagatcg acaagagttt cgtcaacgat ctggttaccg acgaaaacga caccgccatt
     1741 gtccaggcca tcattgccat ggcccacaag ctcaatctgg aagtggtggc cgaaggcgtg
     1801 gagacgcggg agcaggagcg caagctgcgg gagaatggct gcgacattgc gcagggctat
     1861 ttgtatttcc gccccatgtc ggtggagcag gtgaatgcat tgctggccgg caaggcgccg
     1921 ggactacccc tggtataacc cgcacgcctg gctcttcccg tccgcttgaa tctgccgcag
     1981 gcgccatttg actgaacatt gatccaggta aaggcccgat cggacagaat tcgctattaa
     2041 tggcgaaata gaggggattc cctttgtgca atcccgctgc ccctgtcccc gctcactatt
     2101 ctggccggtt ccatga
//
LOCUS       DASUEG010000272         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1487418, whole genome shotgun sequence.
ACCESSION   DASUEG010000272 DASUEG010000000
VERSION     DASUEG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487418"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..>2178)
                     /locus_tag="VFX11_05055"
     CDS             complement(10..>2178)
                     /locus_tag="VFX11_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4938018.1"
                     /translation="GPGVRVDTFGYQGYATTPRYDSLLAKIIVHTNGDIAAALAKAWR
                     ALCECRINGVPTNLALLKNLLQHAEVLANRIHTRFIDQHLAELLEDKMHPQRSPQTLV
                     QASAAANPDNVTTEHALHAPMQGTVVNVEVKNGASVRKGQTLLILDAMKMEHVIAAPH
                     AGIVQGCALKKGDAVLEQQMLLQLLPTESDDSEAVVTDIVDPDFIRPDLAEVVQRHAY
                     GHDENRADAVAKRRATGQRTARENIADLCDEGSFVEYGPLAIAAQRRRRTLQDLIEKT
                     PGDGMVCGIGRVNGHQFGDAGQCVLMTYDYTVMAGTQGIQNHRKKDRMFELAQQWQLP
                     VVLFSEGGGGRPGDTDGLGSSASLDCLAFFLFAKLSGLVPLVGIASGRNFAGNAALLG
                     CCDVVIATRNANIGMGGPAMIEGGGLGVYAPEQVGPTSVQVQNGVIDLLVDDEADAVR
                     VAKRYLSFFQGDLAHWQAPDQRLLRTLIPENRLRAYDVRAVIHGLADCDSVLELRAQF
                     GLGMITAFARVEGRAVGIIANNPQYLAGAIDAPGADKAARFMQLCDGFDIPILFLCDT
                     PGIMVGPEVEKTALVRHVSRLFVTAASITVPFFTIVLRKAYGLGAMTMAGGTLKAPLF
                     TVSWPTGEFGAMGLEGAVKLGFRKELDAVSDPQQRDELFRTMVADMYERGKAVNNATF
                     FEFDDVIDPAQSRQWIMTALRSAPEPVKRKGKKRPCIDTW"
BASE COUNT          422 a          714 c          630 g          412 t
ORIGIN      
        1 caggtgccat taccaggtat cgatgcaggg gcgtttcttg cctttgcgtt ttaccggctc
       61 tggcgccgaa cgcagcgccg tcatgatcca ctgccgcgac tgcgccggat cgatcacgtc
      121 gtcgaattcg aaaaaggtcg cgttgttcac cgccttgccg cgctcgtaca tgtccgccac
      181 catggtgcgg aacagttcat cgcgctgctg cgggtcgctc actgcatcca gctccttgcg
      241 aaagccgagt ttcactgcgc cttccaggcc cattgccccg aattcgccgg tgggccagga
      301 cacggtgaac agcggcgcct tcaacgtgcc gcccgccatg gtcatggcac ccaggccata
      361 ggccttgcgc aacacgatgg tgaagaacgg cactgtgata ctggcggccg tgacaaacag
      421 gcgcgacacg tgacgcacca gcgccgtctt ttccacttcc ggcccgacca tgatgcccgg
      481 cgtgtcacac aggaacagaa tgggaatatc gaaaccgtcg cacagctgca tgaagcgcgc
      541 ggccttgtcg gcaccaggcg catcgatggc accggccaga tactgcggat tgttggcgat
      601 gatgccgacc gcacgacctt cgacgcgggc gaacgcggtg atcatgccca gtccaaactg
      661 cgcgcgcagt tccagcacgg aatcgcaatc cgccagaccg tgaatcacgg cacgcacatc
      721 gtaggcgcgc aggcggtttt ccggaatcag ggtgcgcagc agacgttggt ccggtgcctg
      781 ccagtgcgca aggtcgccct ggaagaaaga caagtagcgt ttggcgacac ggacggcgtc
      841 cgcctcatcg tccaccagaa gatcaatcac accgttctgc acctgcaccg acgtcgggcc
      901 cacctgctcc ggcgcgtaca cgcccaagcc accaccctcg atcatcgcgg gcccgcccat
      961 gccgatattg gcgttacggg ttgcgatcac cacatcgcag caacccagca gcgcggcgtt
     1021 gccggcaaaa ttgcgccccg aggcaatgcc taccagcggc accagccccg acagctttgc
     1081 aaacaggaaa aacgccagac aatccagact ggcggatgaa cccaggccat cggtatcgcc
     1141 aggacgaccg ccgccacctt cgctgaacag caccaccggc aattgccact gctgcgccag
     1201 ctcgaacatg cggtctttct tgcgatgatt ctggatgccc tgggtaccgg ccatcactgt
     1261 gtagtcatag gtcatcagca cacactggcc ggcatcacca aactggtggc cattaacgcg
     1321 gccgatgccg cacaccatac cgtcgcccgg agttttctcg atcagatcct gcaacgtgcg
     1381 gcgacgacgc tgggccgcaa tcgccagcgg accgtattcc acgaagctgc cctcatcgca
     1441 cagatcggca atattttccc gcgcggtgcg ctggccggtg gcgcggcgtt ttgccaccgc
     1501 atcggcgcga ttttcatcgt gcccgtaagc gtgacgctgc accacttcag ccagatcggg
     1561 gcggatgaaa tcaggatcga caatgtccgt taccaccgct tcgctgtcat cactttccgt
     1621 gggcagtagt tgcagcagca tctgctgctc cagcacggca tcgcctttct tcagcgcgca
     1681 gccctgtaca atgcccgcat ggggtgcggc aatgacgtgc tccattttca tggcatccag
     1741 aatcagcagc gtctggcctt tacgcacact ggccccgttt tttacttcca cgttcacgac
     1801 agtgccttgc atgggcgcat gcagggcgtg ctcggtggtg acgttgtcag gattcgcggc
     1861 tgccgacgcc tgcaccagcg tttgcggcga acgttgcgga tgcattttgt cttccagcaa
     1921 ttccgccaga tgctgatcga taaagcgcgt gtgaatgcga ttggcgagca cctcagcatg
     1981 ctgcagcaga tttttcagca gcgccaggtt ggtggggaca ccgttgatgc ggcactcgca
     2041 cagtgcgcgc caggcttttg ccagcgccgc cgcaatatcg ccgttagtgt ggacgatgat
     2101 cttcgccagc agggaatcgt agcgcggggt ggtggcgtat ccctgatagc cgaacgtgtc
     2161 cacgcgcaca ccggggcc
//
LOCUS       DASUEG010000273         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_358511, whole genome shotgun sequence.
ACCESSION   DASUEG010000273 DASUEG010000000
VERSION     DASUEG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VFX11_05060"
     CDS             <1..2157
                     /locus_tag="VFX11_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938019.1"
                     /translation="TTMTMSTRQRTYPHHILVIDDKADLLASLKSLLEIHGFRVSTAT
                     STEQAIDSLKKSNFDLVLLDILMPQLSGHEVLRFVQEKRADIPVIVISGDYSFSSVST
                     ALKRGACDFIRKPYVAEELINAIDNALDKRRLEFEFKTMQQRIQQSEKMHRFMVNNSP
                     DIIYILNERGHITFINDSVEKLLNLPKDELMGSHFSALTGDDESRNHPFTFNERRASG
                     RKTQNREMHLRKRLCINGDGSATTLSVPFEINAMGIYEVERLTGKRQFRGTYGIARDI
                     TDRKQAENFMRFQAYHDLLTGLPNRSLFRDRLSLAISHAKRAGTRVAVMFLDLDRFKI
                     INDTLGHNIGDLLIQAVSRRISECLREGDTLSRFGGDEFTLLLPNLKNQNDAAIIARK
                     ITTELKQVFFIEQHEIVISGSIGIAMYPEHGDQIDALIQNADIAMYHTKSHGKNGHQF
                     FSEQMNQAFADKLSIERDLRGCLEQQQLFLEYQPIINVSTGNVYALEAYLRWNHPQRG
                     LVMPNDFLKIAEETGQLIDMGTWVVNRVCEDLQQWQNPILKIAINFNPLQVEHPDFEN
                     MLMCAMQKYNVSPNQIEIEITEQLLIRDQTSIAAKLRRLTRLGFSIAVDNFGTGFSSL
                     SCLHQLPINTIKLDQSFIRHIGDDTKSGDACIVNAIAAMANGLNLNLVAEGVETLAQK
                     NYLTHLGCFTMQGHLFQRPVSSEDARNITIAPPLVQ"
BASE COUNT          539 a          689 c          571 g          432 t
ORIGIN      
        1 acgacaatga cgatgtccac ccggcagcgt acctatccac atcacatact cgtgatcgac
       61 gacaaagccg atctgctggc gagtctgaaa tccctgctgg aaatccacgg tttccgcgtt
      121 tccaccgcca cttccacgga acaggccatc gacagcctga aaaaaagcaa cttcgacctg
      181 gttttgctgg acatcctgat gccgcaactg agcggccatg aagttctgcg cttcgtgcag
      241 gaaaagcgcg ccgatattcc agtgatcgtg atcagcggcg actattcctt ttcctcggtg
      301 tccactgcgc tcaagcgcgg tgcctgcgac ttcatccgca aaccctatgt ggcggaagaa
      361 ctgatcaacg ccatcgacaa tgcactggac aagcgccggc tggaattcga attcaagacc
      421 atgcagcagc gcatccagca gtcagagaag atgcaccgtt tcatggtgaa caattcaccg
      481 gacatcatct acatcctgaa tgaacgcggc cacatcacct tcatcaacga cagcgtggaa
      541 aaactgctga acctgcccaa ggatgagctg atgggcagcc atttcagtgc tttgaccggt
      601 gatgacgaat cccgcaatca tccgttcacc ttcaatgaac ggcgcgccag cggccgcaag
      661 acccagaacc gggaaatgca tctgcgcaag cgtttgtgca tcaatggcga tggcagcgcc
      721 accacgctgt cggtgccgtt cgaaatcaat gccatgggga tctacgaagt agagaggctc
      781 accggcaagc gccagttccg cggcacctac ggcattgccc gcgacattac tgaccgcaag
      841 caggcggaaa acttcatgcg cttccaggcg tatcacgatc tgctcaccgg cctgcccaac
      901 cggtccctgt tccgcgatcg cctgtcactg gccatttccc acgccaagcg tgcgggaacc
      961 cgggtagcgg tgatgtttct ggacctggac cgcttcaaga tcatcaatga caccctgggc
     1021 cacaacattg gcgacctgct gatccaggca gtgtcgcgcc gcatcagcga atgcctgcgg
     1081 gaaggcgaca ccctgtcgcg cttcggcggc gacgaattca ccctgctgct gcctaacctg
     1141 aagaaccaga acgacgcggc catcattgca cgcaagataa caaccgaact gaagcaggtt
     1201 ttcttcattg aacagcatga aattgtgatt tcaggttcca ttggcattgc catgtatccc
     1261 gagcatggcg accagatcga tgccctgatc cagaatgccg atatcgccat gtaccacacc
     1321 aagtcccacg gcaagaacgg acaccagttt ttcagcgagc agatgaacca ggcattcgcg
     1381 gacaaactgt cgatcgagcg cgatctgcgc ggttgcctgg agcaacagca actgtttctg
     1441 gaatatcagc cgatcatcaa tgtcagcacc ggcaacgttt atgcgctgga agcctatctg
     1501 cgctggaatc acccgcaacg gggactggtg atgccgaatg atttcctgaa aattgccgag
     1561 gaaaccggcc agctcatcga catgggcacc tgggtggtga accgcgtatg cgaggatctg
     1621 cagcaatggc agaacccgat cctgaaaatc gctatcaact tcaacccgtt gcaggttgag
     1681 catccggatt tcgagaacat gctgatgtgc gccatgcaga aatacaatgt gtcgcccaac
     1741 cagatcgaga tcgaaatcac cgagcagctg ctgatccgcg accagaccag cattgccgcc
     1801 aagctgcgtc ggctgacccg gctgggcttt tccattgccg tggacaattt cggcaccggt
     1861 ttttcgtcgc tcagctgcct gcaccagctg cccatcaata ccatcaagct ggaccagtcc
     1921 ttcatccgcc atatcggcga cgacaccaag agcggcgatg cctgtatcgt caacgccatc
     1981 gcggccatgg ccaacggcct gaacctgaat ctggtggctg aaggggtgga aaccctggcc
     2041 cagaagaact acctcaccca cctgggctgt ttcaccatgc agggccatct gttccagcgc
     2101 ccggtgagca gcgaagacgc acgcaatatc accatcgccc cgccgctggt gcaataggcc
     2161 gccacaccgg cacgaaaaaa atgatcccga tcattttgtc cacgaccgtt tcctgacatg
     2221 atcaaccgat c
//
LOCUS       DASUEG010000274         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1199055, whole genome shotgun sequence.
ACCESSION   DASUEG010000274 DASUEG010000000
VERSION     DASUEG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199055"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="lepA"
                     /locus_tag="VFX11_05065"
     CDS             <1..1009
                     /gene="lepA"
                     /locus_tag="VFX11_05065"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX4938020.1"
                     /translation="IKDIHGAPVGDTITHAKTPEVAMLEGFKKSKPQVYAGLFPVTSD
                     DYDRFRDALEKLSLNDASLHYEPETSDALGFGFRIGFLGMLHMEIIQERLEREYDLDL
                     ITTAPTVIYEMLLKNGELYYVDNPSKLPDPSKVEDFREPVADVNILVPQEYLGNVITL
                     CSEKRGSQKNLQFMGKQVAVHYEIPMSEVVLDFFDKLKSTSRGYASLDYSFKRFESSN
                     LVRVDVMINGDKVDALAVICHRDQAEFRGRVLTEKMKELIPRQMFDVAIQASIGSKII
                     ARQTVKALRKNVLAKCYGGDVSRKRKLLEKQKEGKKRMKQVGSVEIPQEAFLAVLKVD
                     K"
     gene            1013..1762
                     /gene="lepB"
                     /locus_tag="VFX11_05070"
     CDS             1013..1762
                     /gene="lepB"
                     /locus_tag="VFX11_05070"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX4938021.1"
                     /translation="MDFDFALILVVLVGVTGFVYLLDILMFRHSRPEGAPVPVWIEYP
                     VSFFPVLLVVLVLRSFLVEPFKIPTGSMIPTLLVGDYILVNKFAYGLRLPVLGTEIVP
                     IGKPQRGDIMVFKYPENPSINYIKRVIGVPGDKITYRNKQLFINGEPVPQTLEAQLPA
                     GRPEFKIYTEQLDGVKHSIMVTLQRSPEAEESWIVPEDGYFMMGDNRDNSRDSRYWKF
                     VPDELIVGKAFAIWMHMPGWIPSFERNQLVE"
     gene            1781..2158
                     /locus_tag="VFX11_05075"
     CDS             1781..2158
                     /locus_tag="VFX11_05075"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX4938022.1"
                     /translation="MDTIKRQNGMSTLGMMTVALAIVFAGVTAMKLWSPYYDDLAVKT
                     ALENIAKEESTKAMGPGEIRQTINKRLQVNQVELSKDDIQIKKEDGVIKIDIIYERRV
                     PMYGNIDAMVKFNHSATVNARGA"
     gene            2155..>2631
                     /gene="rnc"
                     /locus_tag="VFX11_05080"
     CDS             2155..>2631
                     /gene="rnc"
                     /locus_tag="VFX11_05080"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX4938023.1"
                     /translation="MIPDSLISKIGYGFQRADLLELALTHRSFSGKDNNERLEFLGDS
                     ILNFTIGEILFHQFPQAKEGQLSRLRASLVKGVTLTKIAQQLDLGPFLKLGPGELKSG
                     GSRRDSILADAVEAIIGAVFLDGGMEQCKALILRWFAPRLQGLTLEDTVKDNKTRLQ"
BASE COUNT          608 a          685 c          786 g          552 t
ORIGIN      
        1 catcaaggat atccatggcg caccggtggg tgacaccatt acccatgcca aaacccccga
       61 agtcgccatg ctggaaggct tcaagaagag caagccgcag gtgtacgcgg gcctgttccc
      121 ggtgacctcc gacgattacg accgtttccg cgacgcgctg gaaaaactga gcctgaacga
      181 tgcctcgctg cattatgagc cggaaacgtc cgatgccttg ggcttcggtt ttcgcatagg
      241 cttccttggc atgctgcaca tggaaatcat ccaggagcga ctggagcggg aatacgacct
      301 cgatctgatt accactgcgc ccacggtaat ctatgaaatg ctgctgaaaa acggcgaact
      361 gtactatgtc gacaacccgt ccaaactgcc ggacccctcc aaggttgagg atttccgtga
      421 accggtggcg gacgtgaaca tcctggtgcc gcaggaatac ctgggcaatg tgatcaccct
      481 gtgttcggaa aagcgcggtt cccaaaagaa tctgcaattc atgggcaagc aggtggcggt
      541 gcactacgag attcccatga gcgaagtggt gctggatttc ttcgacaagc tgaagtccac
      601 cagccggggt tatgcctcgc tggactattc cttcaaacgc ttcgagtcca gcaatctggt
      661 gcgggtggac gtgatgatca acggcgacaa ggtggatgcg ctggcggtga tctgtcaccg
      721 ggatcaggcc gagttccgcg gacgtgtgct gaccgagaaa atgaaggaac tgattccgcg
      781 ccagatgttc gacgtggcga ttcaggcctc tatcggcagc aagatcattg cccggcagac
      841 agtgaaggcc ttgcgcaaga acgtgctggc caagtgctac ggcggcgacg tcagccgcaa
      901 gcgcaaactg ctggagaagc agaaagaagg caagaagcgc atgaagcagg tgggaagtgt
      961 ggaaattccc caggaagcat tcctcgcagt actcaaggtg gacaagtaac caatggattt
     1021 tgattttgcg ttgattctgg tggttctggt gggggtcacc ggctttgttt atctgctgga
     1081 catcctgatg ttccgtcaca gccgtcccga gggtgcgccg gtaccggtgt ggatcgaata
     1141 cccggtgtcg tttttcccgg tgctgctggt ggtactggtg ttgcgctctt tcctggtgga
     1201 accgttcaag atccccaccg gctctatgat tcccaccctg ctggtgggtg actatattct
     1261 ggtgaacaag tttgcctatg gcctgcgcct gccggtactg ggcacagaga tcgtgccgat
     1321 cggcaagccc cagcgcggcg acatcatggt gttcaagtac ccggaaaacc ccagcatcaa
     1381 ttacatcaaa cgggtgatcg gcgtgccggg cgacaagatt acctatcgca acaagcagct
     1441 gttcatcaac ggcgagccgg tgccccagac cctggaagcg cagcttccgg cgggcagacc
     1501 tgaatttaaa atctatactg aacaactgga tggggtgaag cattccatta tggtcaccct
     1561 gcagcgttcg cccgaggcgg aagaatcatg gatcgtgccg gaagacggct atttcatgat
     1621 gggtgacaac cgggacaaca gtcgtgatag ccgttactgg aaattcgtgc ccgacgagct
     1681 gattgtgggc aaggccttcg cgatctggat gcatatgccg ggctggattc cgagcttcga
     1741 acgcaaccag ctggttgagt gaatatttga ggtgacggtc atggatacaa taaaaagaca
     1801 gaacggtatg tcgacgctgg gcatgatgac ggtggcgctg gcaattgtgt ttgctggcgt
     1861 tacagcaatg aagctctgga gtccgtatta cgatgacctt gcggtgaaaa ctgcgctgga
     1921 aaacattgcc aaggaggagt ccaccaaggc catggggccg ggcgaaatcc gccagaccat
     1981 taacaaacgg ctgcaggtca atcaggtgga gttgtccaag gacgacatcc agatcaagaa
     2041 agaggatggt gtcatcaaga tcgatattat ctatgagcgc cgcgtgccca tgtacggcaa
     2101 cattgacgcc atggtgaagt tcaaccactc cgctaccgtc aacgcgcggg gcgcatgatc
     2161 ccggacagtc tgatcagcaa gatcggttac gggtttcagc gggcagacct gctggaactc
     2221 gctctcaccc accgcagttt cagcggcaag gacaacaatg aacgcctgga atttctgggc
     2281 gattcgattc tgaatttcac catcggcgag atcctgtttc accagtttcc ccaggcgaag
     2341 gaaggtcagt tgagccggtt gcgggccagt ctggtgaagg gcgttaccct gaccaagatc
     2401 gcgcagcagc tggatctcgg cccttttctg aaactgggcc ctggtgaact gaaaagtggc
     2461 ggttcacgga gagattcgat tctggccgat gcggtggaag ccatcatcgg tgccgtattt
     2521 ctggatggcg gcatggagca gtgcaaggcg ctgattctgc gctggttcgc accccgcctg
     2581 cagggactta cgctggaaga cactgtcaag gacaataaaa cccgcctgca g
//
LOCUS       DASUEG010000275         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2044930, whole genome shotgun sequence.
ACCESSION   DASUEG010000275 DASUEG010000000
VERSION     DASUEG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044930"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFX11_05085"
     CDS             complement(<1..235)
                     /locus_tag="VFX11_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4938024.1"
                     /translation="MNRWIKRSLAGVAITVAIGASTLGGAAIYGDRKMKRSIELPEYP
                     IPLLNDSASIEHGRYLFQARGCAECHGENGGGRV"
     gene            555..1259
                     /locus_tag="VFX11_05090"
     CDS             555..1259
                     /locus_tag="VFX11_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4938025.1"
                     /translation="MSVIRSLHLLFALVIPALLGIISGALWALCTADRRRTTNAVMRV
                     VGWLAPRLAGLRIQCSNRHFLAQRPAVVVFNHQSGLDPVILCALLQRDAVGVAKQPLA
                     RNLLLGPLLRFPGTIFLGEKTERRTRVLEQAQVALDSGLSIVMAPEGTRVQSQSGVGR
                     FRSGACEIAQRCGVPVLPVVIHNSGERLAARGRQLFPGPVRVTVLPPRRIAPDADLEK
                     ATRELEQAYVEALAQA"
     gene            1234..1962
                     /locus_tag="VFX11_05095"
     CDS             1234..1962
                     /locus_tag="VFX11_05095"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HEX4938026.1"
                     /translation="MWKHWHKPEWRMTRALALGAMLLTLLPWALSAADWTLRSKTDAA
                     VQLWTDDSRTPFRHVRAETVIAAPILPLLELLQDPASQPHWLPYTHQVEILQQTAPEQ
                     TLVRFETQARWPFAARDAVTLFQVSQPEASTIRIDMLNRPDAIPPHAGIERIRQAEGH
                     WQLQALDQCRTQVRYEAGNRWGGNVPQWLVDRMNAQLAQEALQNLRKWAPDHYHDYSR
                     PEHSRPEHSRPDYLQKVELHAHCD"
     gene            1946..3397
                     /locus_tag="VFX11_05100"
     CDS             1946..3397
                     /locus_tag="VFX11_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938027.1"
                     /translation="MHTVTENTRGAIRILLLASVVICMGACSVLESHPSAGTTASPLP
                     QCQQELQQLQRTIDRSGVNDAQYVPVQNFPTYRSNRFWSSFAADELNPEQQQYWRRQL
                     HTLGMDSLRIEARNLPTGALPFSSDTFFARCDEPLWQASLQQPLSPQQLQIPDSYSDL
                     QRFFGIYALTSKGALGSIRQYQQEMRERISGDTAFDADHSVLYQPQIADDGSDIRPTR
                     DPLGIPQFSASQLQALLNRHAPQWQVEQRSDADRIGAARWQREKRRIDGEQPIVYAYP
                     SYVRSRQGVLLQLNYTAWFAERPKPKRFDWYGGALDGLIWRVTLRPDGGVLFYDSIHP
                     CGCYHSLHRPQNSALDIPAGKDEPLLVFDSPLSDRHANPVLLVQADTHYLLKVMAQPD
                     KAPQALNYRLAPYDDLRSLPAGAAGKRGPRHRNWFDADGLIAASARFERFFLWPLGVP
                     SAGARRQQGHHAIAFVGRRHFDEAWLEDFLGLR"
     gene            3529..4266
                     /locus_tag="VFX11_05105"
     CDS             3529..4266
                     /locus_tag="VFX11_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938028.1"
                     /translation="MTTPHTLIPHTPKFIRFLLFLLLSLSTLPSRADIITFNFLALLD
                     GTTPVTGQGADGSAWNGPAVEGSYRFFNWTQQDLTLSVAAEYWPEDAEGNMTTGVPAY
                     VYLQQEYAGLGACDVPGCGSTALNYLDRLILGFSAQVELIGIAFNTGADDSGISPDDD
                     DYRAYNTRISMSGGPDGPMGTEMGTGKYWQGSLSGNQLDLYMPEGGSPYIESITVRTQ
                     EVSEPASLTFIVLALALVWLHRRRARP"
     gene            complement(4342..>4752)
                     /locus_tag="VFX11_05110"
     CDS             complement(4342..>4752)
                     /locus_tag="VFX11_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX4938029.1"
                     /translation="DVARFKLTKSKEDIRPGDRLIPTEERKLDSVFHPRSPGSDMQGH
                     IIHVFSGVRNVSQYDVVVLNLGDRDGISIGDVLAVHGKGETVKDQVTGELVKLPDERR
                     GVLMVFRTFEKVSYGLILRAEAPLQVLDVVKNPK"
BASE COUNT          923 a         1535 c         1392 g          902 t
ORIGIN      
        1 acacgcggcc tccgccattt tcaccatgac actccgcaca gccccgcgcc tgaaacagat
       61 aacggccatg ctcgatgctg gcgctgtcat tcagcagtgg aatcggatac tccggcaatt
      121 cgatggagcg cttcattttc ctgtcgccgt aaatggccgc accacccagg gtgctggcgc
      181 cgatggcaac ggtgatggcc acaccggcca gtgatcgttt gatccagcgg ttcattgtct
      241 gccccttcgg gaaacgggag taatcccgga taagaaaagt cacaggttga aataaaagac
      301 cgcaggacgc agctcgcgcg ttgcagcttt tttgccgcgc aaaaaacggc gccttgtgcg
      361 gcggcgccaa gaataagcgc gcttgccggg cggtgctatg tacaaatcac gtacaatcgc
      421 cggccgtatg cccaattgct tttgtgatta tttgcagaat ggtcccggct ttccggcgct
      481 gcgccgaaat gccatgactt gcgcacggtt ccggtggaaa attgctggcc agtgacatca
      541 aggattgaag ttgtatgtct gtcatccgtt ccctgcacct gctgtttgcc ctggtgatac
      601 ccgcgctgct gggcatcatc agtggtgccc tctgggcgct ctgcactgcg gatcgccgtc
      661 gcaccaccaa cgccgtgatg cgggttgtgg gctggctggc accgcgcctt gccgggttgc
      721 gcatccagtg cagcaaccgc cattttctgg cgcagcgacc ggccgtggtg gtattcaatc
      781 atcaaagcgg gctggacccg gtgatcctgt gcgcgctgtt gcagcgcgac gcggtgggtg
      841 tggccaaaca gccgctggcg cgcaatctgc tgctggggcc gctgctgcgt tttccaggca
      901 cgatttttct gggagaaaag accgaacgcc ggacccgggt gctggagcag gcgcaggtgg
      961 cgctcgacag cggcctgtcc atcgtgatgg cgccggaagg gacccgggta cagagtcagt
     1021 ccggcgtcgg ccggttccgc agcggtgcct gcgaaattgc ccagcgctgt ggcgtaccgg
     1081 tgctgcccgt cgtaattcac aacagcggcg agcggctggc ggcacggggc cggcaactgt
     1141 ttcccggccc ggtgcgggtg accgtactgc caccacggcg gattgcgccg gatgcggatc
     1201 tggaaaaagc cacccgcgaa ctggagcagg catatgtgga agcactggca caagcctgaa
     1261 tggcgcatga cccgcgcgct ggcgctgggc gcaatgctgc tgacgttgct gccctgggca
     1321 ctgtccgccg ctgactggac cctgcgttcg aaaactgacg ccgccgtgca actctggacc
     1381 gacgacagtc gcacgccctt ccgccacgtg cgtgccgaaa ccgttatcgc cgcccccatc
     1441 ctgccgctgc tggaactgct gcaggatccg gcgtcgcaac cgcactggct gccctacacc
     1501 catcaggtgg aaatcctgca gcaaactgcg ccggagcaaa ccctggtgcg cttcgaaacc
     1561 caggcccgct ggcccttcgc ggcgcgcgat gctgtcaccc tgttccaggt gagccagccg
     1621 gaggcatcaa caatccgcat cgacatgctc aaccggccgg acgcgattcc cccgcatgcc
     1681 ggcatcgaac gcattcgtca ggcggaaggc cactggcagt tgcaggcgct ggatcagtgc
     1741 cgcacccagg tgcgttacga agccggcaac cgctggggcg gcaacgtgcc acaatggctg
     1801 gtggaccgca tgaacgcgca gctcgcgcag gaagcgttgc agaacctgcg taaatgggcg
     1861 ccggatcatt accatgacta ttcaagaccg gagcattcaa gaccagagca ttccagaccg
     1921 gactatctgc aaaaggtgga actgcatgca cactgtgact gaaaacacgc gcggtgccat
     1981 tcgcatcctg ctgctggcca gcgttgtgat ttgcatgggc gcctgctcag tgctggagtc
     2041 gcacccctct gccggcacga cagcctcgcc gctaccacaa tgtcagcagg aattgcagca
     2101 actgcagcgg accatcgacc gttccggcgt caacgatgcg cagtacgtgc cggtgcagaa
     2161 ttttcccacc taccgcagca accgtttctg gtcgagcttt gccgcagacg aactgaatcc
     2221 ggagcagcag caatactggc gtcggcagct gcacacgctg ggcatggaca gcctgcgcat
     2281 cgaggcacgc aacctgccca ccggcgcgct gcctttttcc agcgacactt ttttcgcgcg
     2341 ctgtgatgaa ccgctttggc aggcatcgct gcagcagccg ctctcaccgc aacaactgca
     2401 aatccccgac agctacagcg atctgcaacg gtttttcggc atttacgcgc tgaccagcaa
     2461 gggtgcgctg ggttcgatcc ggcaatacca gcaggaaatg cgtgagcgga tcagcggcga
     2521 caccgcattc gatgcggatc attccgttct ctatcaaccg cagatagcag atgacggcag
     2581 cgacatccga cctacgcgcg atccattggg cattccgcaa ttttccgcca gccaattgca
     2641 agcattgctg aaccggcacg cgccgcagtg gcaggtggaa cagcgcagcg atgccgaccg
     2701 catcggcgcc gcccgctggc agcgggaaaa acgccggata gatggcgagc agcccattgt
     2761 gtacgcgtac ccatcctacg tgcgctcgcg ccagggcgtg ctgctgcaac tgaattacac
     2821 cgcctggttt gccgagagac cgaaaccgaa acgcttcgac tggtatgggg gcgcgctgga
     2881 cggactgatc tggcgggtaa cgttgcgacc ggatggcggc gtcctgtttt acgacagcat
     2941 ccatccctgc ggctgttatc actcgctgca ccggccacaa aattccgcgc tggacattcc
     3001 tgccggcaag gacgaaccgc tgctggtgtt cgacagcccg ctgtccgacc ggcatgccaa
     3061 tccggtgctg ctggtgcagg ccgacaccca ctacctgctg aaagtaatgg cgcagccgga
     3121 caaagcgccg caagcgctga actaccggct tgccccctat gatgacctgc gctcacttcc
     3181 cgccggggca gccgggaaac gcggcccgcg ccatcgcaac tggttcgatg ccgatggcct
     3241 gatcgccgca tctgcccgct ttgaacgttt tttcctctgg ccactgggag tacccagtgc
     3301 cggcgccagg cgccagcagg gccatcacgc catcgccttc gtcggccggc gccatttcga
     3361 cgaggcctgg ctggaagatt ttctcggcct gcgctgaagg agcatctgcc tcaacaacgg
     3421 cgatttgcgc atcgcatcgg cttgctgcta aggtggatca gccccgccgg gtttcctgcc
     3481 tgttgccact ggattggccc tgcggttggc ccagcaatgg aggctgccat gactaccccg
     3541 cacacactta tcccgcatac cccgaagttc atccgtttcc tgttatttct gcttctctcg
     3601 ctttcaacac tgccatcccg cgccgacatc atcaccttca attttctggc tctgctggat
     3661 ggcaccacac ctgtcaccgg ccagggtgct gatggcagcg cctggaacgg gccggcagtg
     3721 gagggcagtt accgcttttt caactggacc caacaggatc tgaccctgag cgtcgccgcc
     3781 gaatactggc cggaggacgc cgaggggaat atgaccacgg gcgtgccagc gtacgtttac
     3841 ctgcagcagg aatatgcggg ccttggcgcc tgcgacgtgc ctggctgcgg cagcaccgca
     3901 ctgaactacc tggaccgcct gatcctggga ttcagcgcgc aggtggaact gatcggcatt
     3961 gccttcaata cgggcgccga cgattcgggc atttcaccgg acgatgatga ttaccgcgcc
     4021 tacaacaccc gcatcagcat gagcggtggt ccggacggcc ccatgggcac tgaaatgggc
     4081 acgggaaaat actggcaggg ttccctgtca ggcaatcagc tggaccttta catgccggaa
     4141 ggcggcagcc cgtacataga gtccattact gtccgaaccc aggaagtgtc cgaacctgcc
     4201 agcctgacgt tcattgtgct ggcacttgcc ctggtttggc tgcaccggcg ccgcgccaga
     4261 ccgtaacctc agtccctaca ccacgactac aacaaaaaag gcctgcatcg caggcctttt
     4321 catcaagcag aacaggaaac gttatttcgg atttttcacc acatccagca cctgcagcgg
     4381 tgcttctgcg cgcaggatca ggccgtaact gactttctcg aacgtgcgga acaccatcag
     4441 cacgccacgg cgctcgtccg gcagttttac cagctcgccg gtcacctgat ccttcacggt
     4501 ttcgcccttg ccatgcaccg ccagcacatc accaatgctg ataccatccc ggtcacccag
     4561 gttcagcacc accacgtcat actggctgac attacgcacg ccgctgaaca catgaatgat
     4621 gtggccctgc atgtcggaac cgggcgagcg gggatgaaac acggaatcca gtttgcgctc
     4681 ttcggtggga atcaggcgat cacccggccg gatgtcttcc ttcgacttgg tcagcttgaa
     4741 acgcgccaca tc
//
LOCUS       DASUEG010000276         7758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1771603, whole genome shotgun sequence.
ACCESSION   DASUEG010000276 DASUEG010000000
VERSION     DASUEG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771603"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1267
                     /gene="odhB"
                     /locus_tag="VFX11_05115"
     CDS             29..1267
                     /gene="odhB"
                     /locus_tag="VFX11_05115"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX4938030.1"
                     /translation="MTTEIKAPQFPEAVADGTVATWHKKPGDSVSRDELLVDIETDKV
                     VLEVVAPHDGVLSKVIKGEGDTVLSQELIAMLEPAGAKVAAAPAKAAEAVAAKTEVAT
                     DAQVEDSWGPAVRKLIAENNLDPAQIKGTGKGGRITKEDVLAHLKGGTAVAKEEARPQ
                     VKTEAAKPAPAGAVPFVAGDREERRVPMTRLRARVAERLVAAQHNAAMLTTFNEVNMK
                     PVMDMRKNYKDVFEKAHGTRLGFMSFFVRAATEALRKYPSVNASIDGNDIVYHGYYDI
                     GVAVSSDRGLVVPIMRDVDKMGFAEIESKIVEYGQKAQAGKLSLEDMTGGTFTISNGG
                     VFGSLWSTPILNPPQTAILGMHKIQDRPMAVNGQVVILPMMYLALSYDHRLIDGKEAV
                     QFLVTLKELVEDPARLLLEL"
     gene            1335..2774
                     /gene="lpdA"
                     /locus_tag="VFX11_05120"
     CDS             1335..2774
                     /gene="lpdA"
                     /locus_tag="VFX11_05120"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4938031.1"
                     /translation="MAKSFDVVVIGGGPGGYAAAIKAAHLGMNVACIDMYINKEGKPA
                     LGGTCANVGCIPSKALLDSTWKFHEAQQDFAVHGIETTGVSMNVGAMIKRKDDIVRKQ
                     TDGVAMLLKANGVTWLQGRGKLLAGKQVEFTAHDGSTETLQAANVILASGSAPIKLPM
                     APLVEGVVVDNSGALEFQSVPKRLGVIGAGIIGLELGSVWNRLGSQVVVLEAMDTFLP
                     MVDRQVGKELLKSLQKQGLDIRLAARVTGTAVANNEVTIEFTDANGEQKLVVDKLIVA
                     VGRRPFTADLFTADSGVNLDERGFIFVRENCATDVPGVYAIGDIVRGPALAHKATEEG
                     VMVAEIIGGHHAAVNYDCIPSVVYTHPEVAWVGKTEEQVKAAGEEYKVGTFAFAANGR
                     AAAANETTGFVKVVADEKTDRVLGVHIVGPQASEIIMQAVIAMEFGASAEDLQMMVFA
                     HPGLSEVFHEACLDVDGRAIHSAKRKRKK"
     gene            2996..4162
                     /gene="sucC"
                     /locus_tag="VFX11_05125"
     CDS             2996..4162
                     /gene="sucC"
                     /locus_tag="VFX11_05125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX4938032.1"
                     /translation="MNLHEYQAKQLFAQYGLPVSQGVAASTPNEAAQAADVIGGNRWV
                     VKAQVHAGGRGKAGGVKLVKSKDEIIDFAKKLLGTNLVTYQTDAKGQPVSKILVESCT
                     DIAKELYLGAVLDRASRRVVFMASTEGGVEIETVAHNTPEKILKATIDPLVGAQPYQG
                     RDLGYALGLNPEQVKQFTKIFLGLAKMFIEKDVSMIEVNPLVITTEGNLHCLDAKLVL
                     DGNALHRHPDLQAMYDPTQEDERERRAAEWDLNYVALEGNIGCMVNGAGLAMATMDLI
                     KLKGGLPANFLDVGGGATKERVTEAFKIILSDESVKAVLVNIFGGIVRCDLIADGVMG
                     AVAEVGVKVPVVVRLEGNNAELGAKKLAESGLNIIAATGFNDAAEKVVAAVRGK"
     gene            4162..5037
                     /gene="sucD"
                     /locus_tag="VFX11_05130"
     CDS             4162..5037
                     /gene="sucD"
                     /locus_tag="VFX11_05130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX4938033.1"
                     /translation="MSVLINKNTKVICQGFTGAQGTLHSTQAIEYGTKMVGGVTPGKG
                     GTKHLNLPVFNTVAEARKETGCDATVIYVPAAFCKDSILEAADAGIPLIVCITEGIPA
                     LDMLFVKEYVVRKGVRLIGPNCPGVITPGECKIGIMPGHIHKPGRVGIVSRSGTLTYE
                     AVNQTTALGFGQSTCIGIGGDPIPGTTFIDALELLQNDSQTEAIIMVGEIGGSAEEEA
                     AAFIKKNVTKPVVSYIAGVTAPAGKRMGHAGAIIAGGKGTAADKFAALEAAGVKTVRN
                     PSELGLAVKEVTGWK"
     gene            5268..6113
                     /locus_tag="VFX11_05135"
     CDS             5268..6113
                     /locus_tag="VFX11_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4938034.1"
                     /translation="MSYQHRPFDTNLMTAEQMWRFTAPLRFYFSPRFFGLDKIDPTRP
                     TLLVGNHTIFGVLDVPLLIAQVYREQGVLIRTLADHAHYDFPGWRSMLDRIGGVEGTR
                     ENCTELMQRGEHVMVFPGGAREVSKRKGEQYQLTWKRRTGFCAMAIANGYNILPFAAV
                     GPDDAYDILLDADDIVQSPVGKLMKKAGLLDKNSFLRGGDILMPIPRGLGLTALPRPE
                     RFYFAIGDEIDVSAYHGRENDEDALFDLRDQVAFSIQDLIGEMLTLREHDKDKSWMRK
                     LLTSL"
     gene            complement(6151..>7758)
                     /locus_tag="VFX11_05140"
     CDS             complement(6151..>7758)
                     /locus_tag="VFX11_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938035.1"
                     /translation="VNTRWREITNIYDIHLTQEAWVKSLHPEERFNVLNKWKDAVGQG
                     AEFRLEYRLLRDDGTEVNVICHAKPLHDSGGLVTGYLGSLSDISELKSVQNQLEQLAL
                     YDPLTKLANRHLFRNRLEKAIRNCGRTQAKLAILFLDIDHFKKINDTMGHDQGDELLR
                     AIAHRLRFCTRPGDTVARMGGDEFTILVPEIQHVNEADAIARKVLDVLKVPIRLTGVE
                     VIITTSIGITIGLDDADDANTLLKNADLAMYRAKAHGRDNYQFFSNEMNVELTRQLAL
                     EGELRQALQQNQFQLYFQPQIDLAENRLTGYEALLRWVHPQKGFIPPSEFIPVAEDSG
                     LILPIGEWVLRTACEHIRLFREQGILPENGHVAVNLSPRQFHDPNLLTVIRDLLQTTG
                     IQSRELEIEITESLLMENVESTIITLNELKDLGIILAIDDFGTGYSSLNYLKRLPIHI
                     LKVDRSFVMDIPHDEDDMEITAAVIAMAHKLGLKVVAEGVELAEQAEFLKENHCDFVQ
                     GYYYGKPMPVSALYENVKNLQLGQRGN"
BASE COUNT         1623 a         2172 c         2410 g         1553 t
ORIGIN      
        1 cgaacgaaca agacgaaaag gaaaccagat gaccaccgaa atcaaagctc cccagttccc
       61 tgaagcggtt gccgatggca cggtcgcaac ctggcacaaa aaacccggag actccgtgtc
      121 ccgtgacgaa ttgctggttg atattgaaac cgacaaggtg gtgctggaag tggtcgcgcc
      181 ccatgacggt gtgctgagca aagtgatcaa gggcgaaggc gataccgtgc tgagccagga
      241 actgatcgcc atgctggagc ccgcgggcgc caaggttgct gccgcgcctg ccaaggcagc
      301 tgaagcggtt gctgccaaaa cagaagtcgc gaccgatgcc caggtggaag acagctgggg
      361 cccggcagtg cgcaagctga tcgcggaaaa caatctcgac ccggcacaga tcaagggcac
      421 tggcaagggc ggccgtatca ccaaggaaga cgtactggct cacctgaaag gcggtaccgc
      481 cgtggcgaag gaagaagcca ggccgcaggt gaagaccgaa gcagccaagc ctgctcccgc
      541 cggcgcggtt ccgtttgttg ccggtgatcg ggaagagcgc cgcgtgccca tgacccgtct
      601 gcgcgcccgc gtcgccgagc gtctggtggc tgcccagcac aacgccgcca tgctgaccac
      661 cttcaatgaa gtgaacatga agccggtcat ggacatgcgc aagaattaca aggatgtgtt
      721 cgaaaaggca cacggtaccc gtctgggctt catgtccttc ttcgtgcgcg cggccaccga
      781 ggcgctgcgc aagtatcctt cggtcaacgc atccatcgac ggtaacgata ttgtctacca
      841 cggctactac gatatcggcg tggcggtttc cagcgatcgc ggtctggtgg tgccgatcat
      901 gcgtgatgtg gacaagatgg gcttcgccga aatcgaaagc aaaatcgtgg aatacggtca
      961 gaaggcccag gccggtaaac tgtcgctgga agacatgact ggtggtacct tcaccatttc
     1021 caacggtggt gtgttcggtt ccctgtggtc cacgccgatc ctgaacccgc cccagacggc
     1081 cattctgggc atgcacaaga tccaggaccg tccgatggcc gtgaatggcc aggtggtgat
     1141 cctgccgatg atgtatctgg cgctgtccta cgatcaccgc ctgatcgacg gcaaggaagc
     1201 agtgcagttc ctggtgaccc tgaaggaact ggtggaagat ccggcacgac tgctgctgga
     1261 actctgatcg acctccacac cgggtggccc tggccgccac ccgcaatgca catggaaaga
     1321 aataggaaac cgacatggca aaatcgtttg atgtggttgt aatcggcggc ggccccggtg
     1381 gttatgctgc agcgatcaag gctgcgcacc tgggcatgaa cgtggcctgc atcgatatgt
     1441 acatcaacaa ggaaggcaaa ccggcgctgg gcggtacctg cgcgaacgtg ggctgcattc
     1501 cgtccaaggc gctgctggat tccacctgga aattccacga ggcgcagcag gatttcgctg
     1561 tgcatggcat cgaaaccacc ggcgtatcga tgaacgtggg cgcgatgatc aagcgcaagg
     1621 acgacatcgt gcgcaagcag accgatggcg ttgccatgct gctgaaggcc aatggcgtca
     1681 cctggctgca gggccgtggc aagttgctgg cgggcaagca ggtggagttc accgcccatg
     1741 acggcagcac cgaaaccctg caggcagcca atgtgatcct tgccagtggt tctgcgccga
     1801 tcaagctgcc gatggcaccg ctggtggaag gtgtggtggt ggataattcc ggcgcgctgg
     1861 aattccagtc cgtgccgaag cgtctcggcg tgattggtgc cggcatcatc ggtctggagc
     1921 tgggcagcgt gtggaatcgt ctgggttcgc aggttgtcgt actggaagcc atggacacct
     1981 tcctgcccat ggtggatcgc caggttggca aggaattgtt gaagagcctg cagaagcagg
     2041 gcctcgacat ccgtctggct gcccgcgtca ccggcacggc cgtggccaac aatgaagtga
     2101 cgattgaatt caccgacgca aatggtgaac agaaactggt ggtggacaaa ctgatcgtgg
     2161 cggtgggtcg ccgtcccttt acggccgatc tgttcacggc tgacagtggt gtgaatctgg
     2221 atgaacgcgg cttcatcttc gtcagggaga attgcgcaac cgacgtgcct ggcgtgtacg
     2281 ccatcggcga catcgtgcgt ggcccggcgc tggcacacaa ggcgaccgaa gaaggcgtga
     2341 tggtggcgga aatcatcggt ggtcatcatg ccgcggtgaa ttacgattgc atcccgtccg
     2401 ttgtctatac gcacccggaa gtggcctggg tcggcaagac cgaagaacag gtcaaggctg
     2461 ccggtgagga atacaaggtc ggtacctttg cgttcgccgc caacggtcgt gctgccgctg
     2521 ccaacgaaac caccggtttt gtgaaagtgg tggcggatga aaaaaccgat cgcgtgctgg
     2581 gcgtgcatat cgtcggtccg caggcgtcgg aaatcatcat gcaggcggtc atcgccatgg
     2641 aattcggcgc cagcgctgaa gatctgcaga tgatggtgtt cgcgcatccg ggtctgtccg
     2701 aagtgttcca tgaagcctgt ctggatgtgg atggcagggc cattcattcc gccaagcgca
     2761 aacgcaagaa gtaacctgtt ttaacgaatc gagccggatt gtggactcaa ctgcaatctg
     2821 gctttttcaa tccgggcagt gactgtccgg tattgagtgc ggttgcccga ttcggcgtgg
     2881 ctgtcggcga gacggcggtg ccttatcccg tgacgcgaat atgacgccgc tccacaaggg
     2941 cggcgacatt cttcaacgtg gagtttccac acaaagtcag atcgatggtg agccaatgaa
     3001 cttacatgag tatcaagcca agcaactgtt cgcgcagtac ggcctgccgg tttcccaggg
     3061 cgtagcggcc agcactccta acgaagcggc gcaggcagcc gacgtcatcg gtggtaaccg
     3121 ctgggtcgtc aaggcgcagg tgcatgccgg ggggcgtggc aaggcagggg gcgtcaaact
     3181 ggttaaatcc aaggacgaaa tcatcgattt cgccaagaaa ctgctgggca ccaacctggt
     3241 gacctaccag accgacgcaa agggtcaacc cgtcagcaag attctggttg aatcctgcac
     3301 cgacatcgcc aaggaactgt acctgggcgc cgtgctggat cgtgccagcc gtcgcgtggt
     3361 gttcatggcg tccaccgaag gtggtgtgga aatcgaaacc gttgcccaca acaccccgga
     3421 aaaaatcctg aaagccacca ttgatccgct ggtaggcgca cagccttacc agggccgcga
     3481 tctgggctac gcactgggcc tgaacccgga gcaggtgaag cagttcacca agatcttcct
     3541 gggcctggcc aaaatgttca tcgagaaaga cgtgtcgatg atcgaagtca acccgctggt
     3601 gatcaccacc gaaggcaacc tgcactgcct ggacgccaaa ctggttctgg acggcaacgc
     3661 tctgcaccgt catccggacc tgcaggccat gtacgatccg acccaggaag acgagcgcga
     3721 gcgtcgcgct gccgagtggg atctgaacta cgtggctctg gaaggcaaca tcggctgcat
     3781 ggtgaacggc gccggtctgg ccatggccac aatggacctg atcaagctga agggcggcct
     3841 gcccgccaac ttcctggacg tgggcggtgg cgctaccaaa gagcgcgtta ccgaggcgtt
     3901 caagatcatc ctgtcggatg aaagtgtgaa agcggtgctg gtgaacatct tcggcggtat
     3961 cgtccgttgc gacctgattg cggacggtgt catgggcgct gtggctgaag tgggcgtgaa
     4021 ggtgccggtc gtcgtgcgtc tggaaggcaa caacgccgag ctgggcgcga agaaactggc
     4081 ggaaagcggt ctgaacatca tcgcagcaac cggattcaac gatgctgctg aaaaagtggt
     4141 tgcagcagtg aggggcaaat aatgagcgtt ttaatcaaca agaacaccaa agtcatctgc
     4201 cagggcttca ctggcgccca gggcacgctg cacagcaccc aggcgatcga gtacggcaca
     4261 aaaatggtcg gcggcgtgac cccgggcaag ggcggcacca agcacctgaa cctgccggtc
     4321 ttcaacaccg ttgcggaagc ccgtaaagaa accggctgcg atgccaccgt gatctacgtt
     4381 ccggctgcgt tctgcaagga ctcgatcctg gaagcggctg acgccggcat tcccctgatc
     4441 gtctgcatca ccgagggcat tcccgccctg gacatgctgt tcgtgaagga atacgtggta
     4501 cgcaagggcg tgcgcctgat cggccccaac tgccctggtg tcatcacgcc gggcgagtgc
     4561 aagatcggca tcatgccggg tcacattcac aagccaggcc gcgtgggcat cgtttcccgc
     4621 tccggcaccc tgacctatga agcggtgaac cagaccacag cgctgggctt cggccagagc
     4681 acctgtatcg gtatcggcgg cgacccgatc cccggtacca ccttcatcga cgcgctggaa
     4741 ttgctgcaaa acgattccca gaccgaagcc atcatcatgg tgggcgagat cggtggcagc
     4801 gcggaagaag aagcggcggc gttcatcaag aagaacgtaa ccaagccggt tgtgtcctac
     4861 atcgcgggcg ttaccgctcc tgctggcaag cgcatgggcc atgctggcgc gatcatcgcg
     4921 ggcggcaagg gcactgcagc ggataaattc gcggcgctgg aagcagccgg tgttaaaacc
     4981 gtacgcaacc cgtccgaact gggactggct gtgaaggaag tgactggctg gaaataatcc
     5041 ggcataggtc atgatccaga aacccggctg tggcatgtcc cggccgggtt ttttcgtctg
     5101 gccatccgtt ttgtggtaac ggaaattgcc ttgcaaaccg ctttcacgtc gcgtctgcca
     5161 atggtttacc ggggtggaca cggcacagta aacacacagg atttcatgcc acgatccctg
     5221 ttctgtccag ggcatcgacc accgaggcat tttccacgag ggaacccatg agttatcaac
     5281 accgtccgtt cgacaccaac ctgatgacgg ccgagcagat gtggcgtttc accgcgccgc
     5341 tgcgcttcta tttcagcccg cgcttcttcg gactggacaa gattgacccc accagaccca
     5401 cattgctggt cggcaaccac accattttcg gcgtgctgga tgtgccgttg ctgatcgcgc
     5461 aggtgtaccg cgagcagggc gttctgatcc gtacgctggc cgatcatgcc cattatgatt
     5521 ttcccggctg gcgcagcatg ctggaccgta ttggcggcgt ggaaggcacg cgggaaaact
     5581 gtaccgaact gatgcagcgt ggcgagcatg tgatggtgtt tcccggcggc gcccgcgaag
     5641 tgagcaagcg caagggtgag caataccagc tgacctggaa gcgccgtacc ggcttctgcg
     5701 ccatggccat cgccaacggc tacaatatcc tgccgtttgc ggcggtgggg ccggacgacg
     5761 cttacgacat cctgctggat gccgatgaca tcgtgcagtc gccggtcggc aagctgatga
     5821 aaaaagccgg tctgctggac aagaacagtt ttctgcgcgg tggcgacatt ctgatgccga
     5881 ttccccgggg gctcggcctg acggcgttgc cacgtcccga gcgtttttat tttgccatcg
     5941 gtgacgagat tgatgtgtcg gcctaccatg gccgggaaaa tgacgaggat gcgctgttcg
     6001 atctgcgcga tcaggtggcg ttttcgattc aggatctgat tggtgagatg ctgacgttgc
     6061 gggaacacga caaggacaag agctggatgc gcaaactgct gacgtcgctt tgatgcagtg
     6121 cggtgtgcag ccggacgggg cgctgattta tcagttgccc cgttgtccca gctgcaggtt
     6181 ttttacattt tcatacagtg cagacacggg catgggtttg ccgtagtaat agccctgcac
     6241 aaagtcacaa tggttttcct tcaggaactc ggcctgttcc gccagctcca caccttcggc
     6301 caccaccttc agccccagct tgtgcgccat cgcaattacc gccgcggtga tttccatgtc
     6361 gtcctcgtcg tgaggaatgt ccatgacgaa cgagcggtcc actttcagga tgtgaatcgg
     6421 cagacgcttc agataattca gcgatgaata gccggtaccg aaatcgtcga ttgccagaat
     6481 gatacccaga tccttcagtt cgttcagggt gatgatggtg ctttccacgt tctccatcag
     6541 caggctttcg gtgatttcga tctccagttc gcggctttga ataccggtgg tctgtagcag
     6601 gtcgcggatc accgtgagca ggttgggatc gtggaactgc cgcggtgaca ggttgactgc
     6661 aacatggccg ttttcaggca aaatcccctg ttcgcggaac aggcggatgt gttcgcaggc
     6721 tgtgcgcagt acccattcgc cgatgggcag gatcaggccg gaatcttcgg ccaccggaat
     6781 gaattccgac ggaggaatga aacctttctg cggatggacc cagcgcaata gcgcttcgta
     6841 gccagtaagg cggttttcgg ccagatcgat ctgcggctgg aagtacagct ggaactgatt
     6901 ctgttgcagt gcctgccgca gttcgccttc cagtgccagt tgccgggtca actccacgtt
     6961 catctcgttg gagaagaact ggtagttgtc gcggccgtgg gccttggcgc ggtacatcgc
     7021 caggtcagca tttttcagca acgtgttggc atcgtctgcg tcatccaggc cgatggtgat
     7081 tccgatgctg gtggtgatga tgacttccac gccggtaagg cgaatcggca ctttcagcac
     7141 atccaacact ttgcgtgcaa tggcatccgc ttcattcaca tgctggattt ccggtaccag
     7201 gatcgtgaat tcgtcgccac ccatgcgcgc cactgtgtcg ccggggcggg tgcagaagcg
     7261 cagccggtgg gcaatggcac gcagcagttc atctccctga tcgtgcccca tggtgtcgtt
     7321 gattttcttg aagtggtcaa tgtccaggaa caggatcgcc agcttggcct gggtacggcc
     7381 acaattgcgg atcgcttttt ccagacggtt gcggaacagg tggcggttgg ccagtttggt
     7441 cagcgggtcg tacagcgcca gttgctccag ctggttctgt acggacttca gttcggaaat
     7501 gtctgacaga ctgcccaggt agccggtcac cagaccgccg ctgtcgtgca ggggcttggc
     7561 gtggcagatc acgttcactt cggtgccatc gtcccgcagc aggcggtatt ccaggcggaa
     7621 ttcggcgccc tggccgacgg catccttcca tttgttcagc acgttgaagc gttcttccgg
     7681 atgcaggctt ttgacccagg cttcctgcgt caggtgaata tcgtagatgt tggtgatctc
     7741 gcgccagcgg gtattcac
//
LOCUS       DASUEG010000277         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_556466, whole genome shotgun sequence.
ACCESSION   DASUEG010000277 DASUEG010000000
VERSION     DASUEG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556466"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1638
                     /locus_tag="VFX11_05145"
     CDS             175..1638
                     /locus_tag="VFX11_05145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX4938036.1"
                     /translation="MIFSKVKWLLGGVLMAGLFHHGAVHAAASQAVLDRVVNGQATFR
                     LSQTTGLAADESVDRDANELIQQTDAMLKRLNTNRQFRLDRAPIVSIEWRGKGRTIKG
                     DAQRPEPTKVREQFNDDTDGFRSYANAGADTLDSFTAYNPTSRNIFEMRFPRSMRASL
                     HSQAMQTGAVHGFNNDAAPADRAIEREEKTELVPLGWSMNDDNRIRIGSLNTRITTWP
                     WRTIVHFSNTCSGTLIGPRHVVTAAHCINPSGTDDFSAFTVRSERNGSDWLGSSAMPG
                     CPNSSTQACPNTGAAYWYFTPSQWRQASVSNREQYDFGIIVLPDRLGDAVGWMGYWYA
                     DVNALETVDKYNRGYPSCNAMSGNQARIDDPADPAMCATCTTDLTVCSANHMYGDAAA
                     CSIANATNTDGDGWNRNVRMTCDASAGHSGSPLYIYGDGNVGSSGSVYYTAHDIQWIC
                     GGTATSSSCANVPRADRLLRMTPEYAEWISYFRDTFP"
     gene            1713..>2270
                     /locus_tag="VFX11_05150"
     CDS             1713..>2270
                     /locus_tag="VFX11_05150"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX4938037.1"
                     /translation="MMVGGTHTLWRRLTALAVMTALTACQSGGLTASQTVTDPVKSTA
                     YIRIQDSESFCSAALIAPRLLLTARHCVQQPGTDQAISPDRLVVGFGENALQSEDHGI
                     AIQAIRLPPQAEFSRAEDLIGTDLALVQLNRAAPIPVMAVAEFQQNAVPEQLTLIGYG
                     VTRSGYYGLRHEIRVKVHAQDKTTLT"
BASE COUNT          486 a          672 c          631 g          481 t
ORIGIN      
        1 caggtccagc cgaagcatta aaaccacctg ttggattgtg cacttgtacc cacccagagt
       61 tcgcaatagg cagctgtttt gcgaagcacg catcccccta tactgcgctc ccctgctgga
      121 cgttcgcccc ggttgtacag ggaaacatta caaccttctc acaaggatct cgttatgatt
      181 ttctccaaag tcaaatggtt attgggtggc gtgctgatgg caggactgtt ccaccatggc
      241 gccgtccatg cggcggcatc tcaggccgtg ctggatcgcg tggtgaacgg ccaggccaca
      301 ttcagactgt cgcagacaac cggtctggcg gcagacgaga gcgttgaccg cgatgcgaat
      361 gaactgattc aacaaacgga tgcgatgctg aaaaggctca acaccaatcg gcagtttcgt
      421 cttgaccgcg ctcccatcgt cagcattgaa tggcgtggca agggccgcac gataaagggt
      481 gacgctcaac gtccggagcc caccaaggtt cgcgaacagt ttaacgatga taccgatggc
      541 ttccgttcct acgccaacgc gggcgccgat accctggaca gctttactgc gtacaacccc
      601 accagccgca atatttttga aatgcgcttt ccccgctcca tgcgggcgtc tctgcactcg
      661 caggcgatgc aaaccggcgc tgtacacggc ttcaataacg acgcagcgcc ggccgaccgt
      721 gccatcgagc gggaagaaaa aaccgagctg gtgccattgg gctggagcat gaacgacgac
      781 aaccgcattc gcatcggctc gctgaatacc cgtatcacca cctggccctg gcgcaccatc
      841 gttcacttct ccaatacctg ttccggtaca ttgatcgggc cgcgtcatgt ggtgactgcc
      901 gcacattgta ttaatccttc aggcaccgat gatttttccg cgtttaccgt acgcagcgag
      961 cgcaatggta gcgactggct gggttcatcg gctatgccgg gctgtcccaa ctcatcgact
     1021 caggcgtgcc ccaatacggg tgcggcctac tggtacttca caccgagcca atggcgtcag
     1081 gccagcgtca gcaaccgaga gcaatatgac tttggcatta tcgtattgcc tgatcgcctg
     1141 ggtgatgccg tgggctggat gggttactgg tacgccgatg tgaatgcact ggaaaccgtc
     1201 gataaataca atcgcggcta tccgtcctgc aacgcaatgt caggcaatca ggctcgcata
     1261 gacgatccgg ctgacccggc catgtgcgcg acctgcacga ctgacctgac cgtttgcagc
     1321 gccaaccata tgtacggcga cgctgccgcc tgctcgattg ccaacgccac caataccgac
     1381 ggtgacggct ggaatcgcaa cgtacggatg acctgcgacg cctccgctgg ccacagtggc
     1441 tcaccgctgt atatctacgg tgatggcaat gtgggttcca gtggcagtgt gtactacacc
     1501 gcgcacgaca ttcagtggat ctgcggtggc acggcaacgt cgtcctcctg cgccaatgtc
     1561 ccccgtgccg accgcctgct gcgcatgacg ccggaatatg cagaatggat ttcctacttc
     1621 cgcgatacct tcccttaaat cctttccaca caggcgctac catgatcgat cggtagcgcc
     1681 tttttcgcat ttttgcgttt gaacgggaga cgatgatggt gggcgggaca cacacattat
     1741 ggcgtcggct gacggcgctt gccgtgatga ctgcactgac cgcctgtcag agtggtgggc
     1801 tgactgcttc gcaaaccgtg accgacccag tgaaatcgac agcgtacatc cgcattcaag
     1861 acagcgagtc tttttgcagt gctgcattga tcgcacccag gctgctgttg accgcacggc
     1921 attgcgtgca gcaaccgggt accgaccagg ctatttcgcc agatcggctg gtggtgggct
     1981 ttggtgagaa tgcactgcaa agtgaggatc atggcattgc aatacaagcg atcaggctac
     2041 ccccgcaagc agaattttcc cgtgcggagg atttgatcgg aaccgatctg gcgctggtac
     2101 aactgaaccg ggcggcgccg attccggtga tggctgtggc cgaattccag cagaatgccg
     2161 tacccgaaca actgacattg atcgggtacg gtgttacccg ctccggctat tacggtttgc
     2221 gtcacgaaat tcgggtgaag gttcatgccc aggacaagac gacactgacc
//
LOCUS       DASUEG010000278         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_600596, whole genome shotgun sequence.
ACCESSION   DASUEG010000278 DASUEG010000000
VERSION     DASUEG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600596"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFX11_05155"
     CDS             complement(<1..345)
                     /locus_tag="VFX11_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX4938038.1"
                     /translation="MLTGKQKKLVPVLMMIGAMGALPASQVHAENLARKVDSTTQQMK
                     QTFSDGVKQGKLETAYLFSEHLNAFDITTEVTGNRAVLTGEVATEVEKELAGEIAKGV
                     EGIVDVENHLVVN"
     gene            complement(536..1651)
                     /locus_tag="VFX11_05160"
     CDS             complement(536..1651)
                     /locus_tag="VFX11_05160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX4938039.1"
                     /translation="MANESPVHTDLQGFHFSSSMEFLLHVIQQLSLARNLSTVQTLVR
                     SAARRLTGCDGATFVLRDGDYCHYLDEDAIAPLWKGQRFPLDQCVSGWCMQNRAQAVI
                     PDVSQDERIPYSIYQHTFIKSMVMVPVRAHAPVGAIGNYWARLHQPRREEVQLLQALA
                     DAVSVTLENIQMYAELEQRVQERTSELQRALQQIHTLSITDELTGLYNRRGFRLLADK
                     TIRRALRGGERFSLVYLDLDGLKLINDSQGHGVGDSMISAIAGVLRQTFRESDILARI
                     GGDEFCVLALGHTEDSAGLRRRLQARLDAFNQSSDKPYQLSCSLGLVTGPMPGVTSLD
                     ELLRHADARMYSDKQARAAAAAPARAVATNRTTTSSA"
     gene            complement(1862..>2636)
                     /locus_tag="VFX11_05165"
     CDS             complement(1862..>2636)
                     /locus_tag="VFX11_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938040.1"
                     /translation="RDIGIALRSGWLTLYYQPKLTAKGLLLKGVEALARWHDPVKGTI
                     SPAQFIAVAERHGLIDALTRHLLDIALQQKQQWQRRGVSFNVAFNLSPLSLADPTLVD
                     WIAQLTAQYGVAPGEITLEVTENALLGELASSIQTLARLRLKGFGVAIDDYGSGFANA
                     QQLSRVPATELKIDRALVHNVATRPQQRTILRNTVEMAHQLQLVTVAEGVEHREDFDL
                     LRQLNVELIQGYFLAKPMPADKLMQWMQTDLASLRKQLR"
BASE COUNT          508 a          769 c          792 g          567 t
ORIGIN      
        1 gttgaccacc agatggtttt caacatcgac gatgccttca acacccttgg caatttcgcc
       61 ggccaattcc ttttcaacct cagtcgccac ttcgcccgtc agcactgcgc ggttgcccgt
      121 tacctccgta gtgatgtcga atgcgttcag gtgctcgctg aacaaatagg ctgtttccag
      181 cttgccctgt ttcacaccgt cactgaacgt ctgcttcatt tgttgcgtgg tggaatccac
      241 tttacgcgcc agattttccg cgtgcacctg tgaggccggc aacgcgccca ttgcaccgat
      301 catcatcaat accggaacca gttttttctg cttgcccgtc aacatgagcg acctcctttg
      361 cagggtcgta atgaggtgag tttcacaaga atcaacgggg aattaacaag gattctggcc
      421 aatgacgaaa attccggcat ggcaggaaca cttgttaatt tcctatcatg attttcgagg
      481 ccgtcagccg gatttggttc cctgcttttt tccagcgaat gttacaggcc ggatatcagg
      541 cactggaggt ggtggtgcga tttgtcgcaa cagcgcgggc cggagcggcc gccgctgcac
      601 gcgcctgctt gtcggaatac atgcgcgcat cggcatgacg cagcagttcg tccaggctgg
      661 tgacgccagg catggggcct gtcaccagac ccagactgca ggacagctgg tagggtttgt
      721 cgctgctctg gttgaaggca tccagcctgg cctgcagccg gcgccgcaga cccgcgctgt
      781 cttcggtgtg gcccagcgcg agcacacaga attcatcgcc gccgatacgg gcaaggatgt
      841 cggattcgcg aaacgtttgc cgcagtacgc cggcaatggc gctgatcatg ctgtcgccta
      901 cgccatgccc ctgactgtcg ttgatcagtt tcagcccgtc cagatccaga tacaccaggc
      961 tgaaccgttc accaccgcgc aatgcgcgcc ggatcgtctt gtccgccagc aggcgaaaac
     1021 cgcgccggtt atacaaaccc gtgagttcgt cggtaatcga cagcgtgtga atctgttgca
     1081 gcgcacgttg cagctcgctg gtgcgctcct gcacgcgctg ttccagttcg gcatacatct
     1141 gaatgttttc cagcgttacc gatactgcat cggccagcgc ctgcagcagc tgaacctcct
     1201 cgcgccgggg ctgatggagc cgcgcccagt aattgccgat ggcaccgacg ggggcatggg
     1261 cacgcaccgg caccatcacc atgcttttga tgaacgtgtg ctggtaaatg ctataaggaa
     1321 tgcgctcgtc ttgcgacacg tccggaatca ccgcctgcgc ccggttctgc atgcaccagc
     1381 cactgacaca ctggtccagc ggaaaacgct ggcctttcca caggggtgcg atggcatctt
     1441 catcaaggta gtgacaatag tcgccgtcgc gcagcacgaa ggtggcacca tcgcaaccgg
     1501 tgaggcggcg ggcggcactg cgcaccagtg tttgcactgt ggaaagattg cgggccagcg
     1561 acagttgctg gatgacatgc agcaggaatt ccatgctgct ggaaaaatga aacccctgca
     1621 ggtccgtgtg gacaggactt tcattggcca tgattgcgag ctcttggcgt tgatgttatg
     1681 tggcgcgcac catgcctttc ctgcgagcgg ttatgacaaa gggcgctggc tgcgacattg
     1741 accatgatag ctgtggaaac agacatgcga ttggattcat tttttcttag cagcgggttt
     1801 ttttagcgcc aacgggaatg agcgggggat attgcgggta ctgccgggcg gtgacgaaag
     1861 gctagcgcag ctgcttgcgc agagaggcca gatccgtctg catccactgc atcagtttgt
     1921 ctgcgggcat gggtttcgcc agaaaatagc cctggatcag ttccacattg agctggcgca
     1981 gcagatcaaa atcctcacgg tgttcgacgc cttcggccac ggtgaccagt tgcagctggt
     2041 gcgccatttc caccgtgttc cgcaggatgg tgcgctgctg cggccgggtg gccacgttgt
     2101 gcaccagggc acggtcgatc ttgagttcgg tggccggcac ccgcgacagt tgctgcgcat
     2161 tggcgaaacc gctgccgtaa tcgtcgatgg cgacgccaaa gcctttcaga cgcagacgcg
     2221 ccagggtctg gatcgaggag gccagttccc ccagcagggc gttttcggtg acttccaggg
     2281 tgatttcgcc gggtgcaacg ccgtactgcg cggtgagctg cgcgatccag tccaccagtg
     2341 tgggatcggc cagcgacagc ggcgacagat tgaaagcgac gttgaaactg acgccccgcc
     2401 gctgccactg ctgtttctgc tgcagcgcga tatccagcag gtgccgggtg agcgcatcga
     2461 tcaggccatg ccgttccgcc accgcgatga actgggcggg ggaaatcgtg ccttttaccg
     2521 gatcatgcca gcgcgccagg gcctcgacac ccttcagcag caggcctttg gcagtgagtt
     2581 tgggctggta atacagcgtc agccagccgg agcgcagggc gatgccgatg tcgcgg
//
LOCUS       DASUEG010000279         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_205269, whole genome shotgun sequence.
ACCESSION   DASUEG010000279 DASUEG010000000
VERSION     DASUEG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205269"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFX11_05170"
     CDS             complement(<1..853)
                     /locus_tag="VFX11_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4938041.1"
                     /translation="MLERVITWSLRNLFLIILFSVAVLAGGIYALWKTPLDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTTAMLAVPQSKVVRGFSFFGSSFVYVIFEDGTD
                     IYWARSRVLEYLNSVSDRLPRGVSPVLGPDATGVGWVYQYALTSDRHDLGQLRALQDW
                     FVRYQLTKAHGVAEVASVGGFVKQYQVTVDPHRLQAYNISLETVMAAIRRSNQDVGGR
                     VVELSEREYMVRGKGYLRGISDLETIVIKAERGTPVLLRDVARIELGPDERRGVTELN
                     GEGEVVAG"
     gene            complement(856..2298)
                     /locus_tag="VFX11_05175"
     CDS             complement(856..2298)
                     /locus_tag="VFX11_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4938042.1"
                     /translation="MNRAALIAGGALLLALGLVIGHWTSTKADNTAGTPTERKPLYYR
                     HPMGLPDTSPVPKKDSMGMDYLPVFAESDATRKILYYRNPMGLSDTSPVPKTDSMGMD
                     YLPVYEGEEAAGKLVRISPEKVQKLGVKLESVQRRQIQRPVRAVGTIEADERTLTTIA
                     PRFEGWVETLHANETGRQIGRGQPLFDVYSPELVAAQQEYLLALEARQSLSGEMASEL
                     LRTARLRLLNWQLTEADIQALERTRKPQRSVSFRSPINGVILEKMVTQGMRFMPGDAL
                     YRLAELKTVWLIANVYEQDLSALQLGQPVTLSVAAFPGESFNGRITFIYPVLADETRT
                     ARVRIELPNPERRLLPGMYGHVNVQPTEDAVPVLSVSDSALMDSGKQQLVLVQVGDGI
                     FEPRPVQAGRRGDGYIEILSGLAEAEQVVVSANFLIDAESNLKAAIGSFGHAGHGASP
                     ATEASKAEHTKHTTPAEAPVSAPEAAHEGH"
     gene            complement(2295..>2470)
                     /locus_tag="VFX11_05180"
     CDS             complement(2295..>2470)
                     /locus_tag="VFX11_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4938043.1"
                     /translation="ELSFRSASAGYQSGKVDFDTLIEAERQIRQARLLGLAAAVKQQR
                     AAAQFEQLTGMQP"
BASE COUNT          524 a          748 c          650 g          548 t
ORIGIN      
        1 tgccagcgac gacctctcct tcgccattga gttccgtcac cccgcgtcgc tcatcgggtc
       61 ccagctcaat gcgcgccaca tcacgaagca ggaccggtgt accgcgctct gcctttataa
      121 cgatcgtttc cagatccgaa atgccgcgta aatagccctt accacgaacc atgtattcgc
      181 gttctgacag ctccaccacc cgaccgccga catcctggtt gctgcgccga atcgcagcca
      241 taaccgtttc aagcgaaatg ttgtaggcct gcagccgatg tggatcgacg gtcacctgat
      301 actgtttgac aaagccgccg acgctagcga cttcggcgac accatgggct ttggtcaact
      361 ggtagcgaac aaaccagtct tgcagggctc gtagttgccc cagatcgtga cggtcactgg
      421 tcagcgcgta ttggtaaacc cagcctacgc ctgtcgcgtc cggacccagc accggggaaa
      481 cgccgcgggg tagccgatcc gataccgagt tcaaatactc aagcacacgc gagcgagccc
      541 agtagatatc ggtaccgtct tcaaaaatga catagacgaa cgacgatccg aagaacgaaa
      601 acccacgcac cactttcgat tgcggcacgg ccagcatggc ggttgtcaat ggataagtga
      661 cttggtcctc gacaacttgc ggtgcctggc ctggatactc ggtgtagaca atcacctgaa
      721 catcggagag atccggcagg gcatccagtg gtgttttcca aagcgcatag atgccacctg
      781 ccaatacggc gacagaaaac aaaatgatca gaaataggtt tcgcagcgac cacgtgatga
      841 cacgctccaa catgatcagt gcccctcgtg agccgcttct ggcgcggata caggcgcctc
      901 cgctggggtg gtatgtttgg tgtgctcagc tttcgatgcc tccgttgctg gtgaggctcc
      961 gtggccggca tgaccgaaac tgccaatggc cgctttcaga ttgctctccg catcgatcag
     1021 gaagttggca ctgacgacaa cctgctctgc ttcggccagc cccgaaagaa tttcgatgta
     1081 gccgtcgcca cgacgacctg cctgcacagg acgaggctca aatatgccat cgccgacctg
     1141 caccagaacc agttgctgtt tgccactgtc catcagcgct gaatccgaca cactcaacac
     1201 cggcaccgca tcctcggtcg gttggacatt gacatgtccg tacatgcctg gcagcaggcg
     1261 acgctcaggg tttggcagct cgatccgcac ccgggccgtg cgtgtttcat ccgccagcac
     1321 gggataaata aaggtgatac ggccgttgaa cgattcgcca ggaaatgcag cgaccgataa
     1381 cgtaaccggc tgccccagct gtagcgccga cagatcctgc tcatagacat tggcaatcag
     1441 ccatactgtc ttcagttcag caagccgata cagtgcgtcg cctggcataa agcgcatgcc
     1501 ttgcgtgacc attttctcga ggatgacgcc attgatagga gaccggaatg aaacgctacg
     1561 ctgcggcttt cgtgttcgct caagcgcctg aatgtctgct tcagtcagtt gccagttgag
     1621 caaccgcaat ctggctgttc gaagcaactc gctggccatc tcacccgaga gcgattgccg
     1681 tgcttccaac gccaacaaat actcttgttg cgcggccacc agttcaggac tgtacacgtc
     1741 gaaaagtggc tggccgcggc caatctgtcg tccggtttcg ttggcgtgca atgtttcgac
     1801 ccagccttca aaccgtggcg cgatcgtggt tagcgtccgt tcgtccgcct caatggttcc
     1861 gacagcgcgc accggccgct gaatttgtcg tcgttgtact gactcaagct tgacaccaag
     1921 cttctgtacc ttttccgggc tgatccgaac caatttgccc gcagcctcct ccccctcata
     1981 gactggtagg taatccattc ccatactgtc tgttttgggt acaggcgatg tgtcagacag
     2041 ccccatgggg ttgcgatagt aaagaatttt tcgtgttgca tcgctctccg caaacaccgg
     2101 caaatagtcc attcccatgc tgtccttttt gggcactggc gacgtgtccg gaaggcccat
     2161 cgggtgccgg taataaagtg gtttacgttc agttggcgta ccggccgtgt tgtctgcttt
     2221 cgtacttgtc cagtggccga tcactaaccc cagtgcgagc agcagcgctc caccggctat
     2281 gagtgcagct ctgttcatgg ttgcatcccc gttaattgtt caaactgtgc tgcggcgcgc
     2341 tgctgtttca cagcggcagc tagaccaagc aatcgcgcct gccggatctg cctttccgct
     2401 tcaatcaggg tgtcgaaatc cacttttccg gattggtagc ctgccgatgc tgagcggaaa
     2461 ctcagttcac
//
LOCUS       DASUEG010000280         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_583110, whole genome shotgun sequence.
ACCESSION   DASUEG010000280 DASUEG010000000
VERSION     DASUEG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583110"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFX11_05185"
     CDS             complement(<1..332)
                     /locus_tag="VFX11_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006030788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938044.1"
                     /translation="MARTDVVADSQVTQWGTFRLGEEIYGVNVMQIREVLRYTEITPV
                     PGAPYYVLGIINLRGNVVTVIDTRTRFGLQPGDVDNNSRIVIVEVDKQVIGMLVDSVA
                     EVTYLNES"
     gene            complement(357..842)
                     /locus_tag="VFX11_05190"
     CDS             complement(357..842)
                     /locus_tag="VFX11_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938045.1"
                     /translation="MAKTAQSSRSSDDPVIQWVTFRLAGETYGINVMQVQEVLRFTDI
                     APVPGASHHVLGIINLRGKVVTVIDTRIRFGLPKGDINDNTRIVIIEASSHVIGILVD
                     SVAEVVYLRQSEIDSTPNIGNDDSSRFIQGVCHKDGELLILVDLDKMLSEEEWAELEV
                     L"
     gene            complement(963..2090)
                     /locus_tag="VFX11_05195"
     CDS             complement(963..2090)
                     /locus_tag="VFX11_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938046.1"
                     /translation="MNHDPHDGDADKDGAQANGTYVNSAASQNDRAILDYLDEILRDT
                     AADTATTAENIVRAPLVAPDNIAAFDVSRPRKPDPVPARAKAEPRQPVTLPFRTAEKP
                     RVVTSPALLNVPSVTQPAEDMLVEAITPQPSIPAAAAPKESTHQEAIPKESIQQESIQ
                     QETARQKTTPQEPVEIIHDLAPSVPAPEPAVTPVTSEPDLLTLETESAAAAELLPRVQ
                     EWHDNGRPAWAQHRFECLIFSVGGLKLAVPLMTLGAIHRIDRKFNALPHQSSWFLGIL
                     QTPAAGNIKVLDTALCVMPERYDPASRDSLGYVITIHGYAWGLACHQVEKSITLEPDQ
                     VKWRTQRGKRPWLAGTVIDHMCALIDTEGFRDLILKAEQSR"
     gene            complement(2096..2896)
                     /locus_tag="VFX11_05200"
     CDS             complement(2096..2896)
                     /locus_tag="VFX11_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4938047.1"
                     /translation="MKIWAVANQKGGVGKTTTVVALGGLLASAGKRVLLIDLDPHGSL
                     TSYFRLNPDDIEQSVFQLFQHQGEVARTLPRQLIRPTGWPRLDLMPASTLLATLERQA
                     VNQGGAGLVISKTLAWLWDDYDFVLMDTPPILGVLLINALAACQYLLLPVQTEFLALK
                     GLERMMRTLQMVMKSQKRELPYCVIPTMFDRRTQASVGSLRQLRQTYEGQIWPAAIGV
                     DTKLRDASRAGLPPNAFDPGSRGVKAYASLLKYLLTQETTEVRKQLVP"
     gene            complement(2909..3904)
                     /gene="motD"
                     /locus_tag="VFX11_05205"
     CDS             complement(2909..3904)
                     /gene="motD"
                     /locus_tag="VFX11_05205"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEX4938048.1"
                     /translation="MIRRKEEEHENHERWVISYADFITLLFAFFVVMYSISSVNEGKY
                     KVLSDSLVTVFHAQSRTIDPVTVGEQQTRASNDQLIQLPVPGEFPAKDDYKYTVEGVF
                     DEVVVRSLKQGEMRDKEIDRLSKLSEQMAITLEQQIKADQVQIKSNGEWIEVNIQASV
                     LFPSGSAVLQPEALQILAQLAAVLKDTSNPIHVEGYTDDIPIETPQFPSNWELSAARA
                     ASVVRLFQSEKLDPARLAAVGYAEFHPVADNKTEEGRARNRRISLVISKNESDGKTAA
                     QAAPDYKLGVSDPRQGGQGQRLRDGGGAEVPVRVIRLEDGGVLFSGDKPPQPAAP"
     gene            complement(3945..4685)
                     /locus_tag="VFX11_05210"
     CDS             complement(3945..4685)
                     /locus_tag="VFX11_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX4938049.1"
                     /translation="MDILSLIGILVGIVAIIGGNAMEGGHISGLANAPAAVIVIGGTF
                     GAAMLQTPMVTFRHAFVVLRWVVFPPVVNMRDNIGKVINWSMTARKEGLLGLEAITEE
                     ESDAFARKGLQLLVDGGEPEAIRSILEVELITQEDRDLHAAKVFEAMGGYSPTIGIIG
                     AVMGLIHVMGNLADPSKLGAGIATAFVATIYGVGLANLLFLPIAAKLKTTIKANSNQR
                     EMIIEGLIAIADGENPRTIELKLEGYVS"
     gene            complement(4690..>5161)
                     /locus_tag="VFX11_05215"
     CDS             complement(4690..>5161)
                     /locus_tag="VFX11_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX4938050.1"
                     /translation="PILLVQHMPGTFTTAFAERLDRLCKIRVKEAEDNDPVRPGWALL
                     APGGKQMLLDARGQAVSVVEGNEKIQYRPSVDVTFGSAARSVKGKILAAVLTGMGHDG
                     REGCKMLKRGGATVWTQNEQSCVVYGMPMAVETAGLSDAVVDLKDLGKYLSELR"
BASE COUNT         1002 a         1544 c         1480 g         1135 t
ORIGIN      
        1 tccgattcgt tcagataagt gacttccgcc acgctgtcca ccagcatgcc gatcacctgc
       61 ttgtccactt ccacgatcac gatgcggctg ttgttgtcca cgtcgcccgg ttgcaggccg
      121 aaacgggtgc gggtgtcgat cacggtgacg acattaccgc gcaaattgat gatgcccagc
      181 acatagtaag gcgcgcccgg caccggtgtt atttcggtgt aacgcagtac ctcgcggatc
      241 tgcatgacgt tgacgccgta aatctcttcg cccaggcgga acgtgcccca ctgggtgacc
      301 tgactgtcgg cgactacatc cgttctggcc atgaattttt ctcctgcctt gaatttttac
      361 agaacttcca gttcagccca ctcttcttcg ctcagcatct tgtccaggtc caccaggatc
      421 agcagttcgc cgtccttgtg gcagacaccc tgaatgaagc gcgaactgtc gtcgttgccg
      481 atgttgggcg tgctgtcgat ttcgctctgg cgcagataca ccacttccgc cacgctgtcg
      541 acgagaatgc cgatcacatg ggagctggct tcaatgatga cgatgcgggt gttgtcgttg
      601 atgtcgcctt tcggcaagcc gaagcggatg cgggtgtcga tgacggtgac gactttgcca
      661 cgcagattga tgatgcccag cacatggtgc gatgcgcccg gcaccggggc gatgtcggtg
      721 aaacgcagca cttcctgcac ctgcattacg ttgatgccgt aggtttcgcc cgccaggcga
      781 aaggtcaccc attgaatcac cggatcgtcg ctggaccggc tgctctgcgc tgtctttgcc
      841 attttcttta cacctgtgtg cgccagaaat cgtcaaggcg cagaacgcgc ctttaacttt
      901 ttgttttcta actatagatc agggcggcgg ggttgccagt gcgcggccat tgccgggaga
      961 tgtcagcggg attgttcggc tttcaggatc aggtcgcgga agccttcggt atcgatcagc
     1021 gcgcacatgt gatcaatcac ggtgccggcc agccaggggc gtttaccccg ctgcgtgcgc
     1081 catttcacct gatccggttc cagggtgatg gatttttcca cctgatggca ggcaagaccc
     1141 caggcgtagc catggatggt gatgacataa cccagactgt cgcgggaagc gggatcgtag
     1201 cgctcgggca tcacgcacag tgcggtatcc agcaccttga tattgccggc tgccggcgtt
     1261 tgcagaatgc cgagaaacca gctgctttga tggggcaagg cattgaattt gcgatcgatc
     1321 cggtgaatgg cgccgagtgt catcaggggc accgccagct tcaggccacc gacggaaaaa
     1381 atcaggcatt cgaaccggtg ctgggcccag gccgggcggc cgttgtcgtg ccactcctgc
     1441 acccgcggca gcaactcggc cgctgctgca gattcggttt ccagggtgag gaggtcgggc
     1501 tcggatgtga cgggcgttac ggccggctcc ggtgccggga ccgatggcgc cagatcatga
     1561 atgatttcaa ccggttcttg cggtgtcgtt ttttgccggg cagtttcctg ctgaatagat
     1621 tcctgctgaa tagattcttt ggggattgct tcttgatgcg ttgattcctt aggcgcggca
     1681 gcagcgggga tagacggttg cggggttatt gcttccacca gcatgtcttc tgccggctgc
     1741 gttaccgacg gcacattcag cagtgccggt gatgtcacca cacggggttt ttccgccgtg
     1801 cgaaacggca gcgtaaccgg ttgacggggt tcggcttttg cgcgggcggg cacgggatca
     1861 ggtttgcgcg gacggctcac atcgaaggcg gcaatattgt ccggcgccac cagcggcgcg
     1921 cgcacgatgt tttccgcagt tgtggcggta tctgccgcgg tgtcccgcag aatttcatca
     1981 aggtaatcaa gaatggcccg gtcgttctgg ctggcagcgc tgttaacgta agtgccatta
     2041 gcctgagcgc cgtccttgtc agcatcgccg tcgtgaggat cgtgattcat gcgggttacg
     2101 gtacaagctg tttccgcacc tcggtggttt cctgcgttaa taaatatttt agcagagagg
     2161 cataggcttt gacgccacga ctgccgggat caaaggcgtt gggcggcaaa cccgccctgc
     2221 tggcgtcccg cagtttggta tcgacaccga tggccgcggg ccagatctgg ccttcatagg
     2281 tttggcgcaa ctggcgcagg ctgcccaccg aggcctgggt gcgccggtcg aacatggtgg
     2341 gaatcacgca gtacggcaat tcacgcttct gggatttcat caccatctgc aaggtacgca
     2401 tcatccgctc cagccctttc agcgccagaa actctgtctg caccggcaac aagagatact
     2461 ggcacgcggc cagcgcgttg atcagcagca cgcccagaat cggcggcgtg tccatcagca
     2521 cgaaatcgta atcgtcccac agccaggcca gggttttgga aatcaccagg ccggcgccac
     2581 cctgattcac cgcctgccgt tccagcgtgg cgagcaacgt agatgctggc atcagatcca
     2641 gccgcggcca gccggtagga cggatcagtt ggcgcggcaa cgtgcgcgcc acttcaccct
     2701 gatgctgaaa cagctggaaa accgattgtt cgatatcgtc cggattcagg cggaaatagc
     2761 tggtaagtga accgtgcgga tcgagatcga tcagcagcac gcgcttgccg gcactggcca
     2821 gcaatccgcc cagcgccacc accgtagtgg ttttgccaac gccgcctttc tgattcgcca
     2881 ccgcccagat tttcatagga ttatctgctc agggtgccgc cggctggggt ggcttgtcgc
     2941 cggaaaacaa gacgccgccg tcttccaggc gaatgacacg caccggtacc tcggcgccac
     3001 caccgtcacg caggcgttgt ccctgcccgc cctggcgcgg atcgctgaca cccaatttat
     3061 aatccggtgc tgcctgggca gcggttttgc cgtcactttc atttttggaa atcaccagcg
     3121 agatgcggcg gttgcgcgcg cggccttcct ctgtcttgtt atcggccacc gggtgaaatt
     3181 ctgcatagcc cacggccgcc agccgggccg gatcgagttt ttccgactgg aacaggcgca
     3241 caacgctcgc cgcccgcgcc gccgacaatt cccagttgga aggaaactgc ggtgtctcga
     3301 tgggaatgtc gtcggtatag ccttcaacgt ggatggggtt gctggtgtcc ttcagaacgg
     3361 cggccagttg cgccagaatc tgcaacgcct ccggttgcaa aaccgcgctg ccactgggaa
     3421 acagcacgct ggcctggatg ttcacttcaa tccactcgcc attgcttttg atctgcacct
     3481 gatcggcctt gatctgctgc tccagcgtga tcgccatctg ctccgacagt ttcgacagcc
     3541 ggtcgatttc cttgtcacgc atctcgccct gcttgaggga gcggaccacc acctcgtcga
     3601 acacaccttc caccgtgtat ttgtaatcat ccttcgcggg aaattcaccc ggcactggca
     3661 actggatcag ctgatcgttg ctggcacggg tctgctgctc gccgacggta acgggatcga
     3721 tggtgcggga ctgggcatgg aatacggtga ccagcgaatc cgacagcact ttgtacttgc
     3781 cttcgttgac agaagatatg gagtacatca cgacaaaaaa cgcaaacagc agggtaatga
     3841 aatccgcata ggaaattacc cagcgttcat ggttttcgtg ctcctcctct tttcgtcgga
     3901 tcatataacg cccctgatag gctttgctgc tcatggcggc gaacctagct cacgtatcct
     3961 tccagcttca gttctatggt gcggggattt tcgccgtcag caatggcaat cagaccctcg
     4021 atgatcattt cccgttgatt gctatttgcc ttgatagtgg ttttgagctt ggccgcaatg
     4081 ggcagaaaca acaggttggc aagtcccacg ccgtagatgg tggcaacgaa cgcggtggca
     4141 ataccagcac ccagcttgct gggatcagcc agattgccca tgacgtgaat cagccccatg
     4201 actgcgccga tgataccgat cgtgggcgaa tagcctccca tggcttcaaa cactttggcg
     4261 gcgtgcaggt cgcgatcctc ctgggtgatc agttccactt ccagaatgct gcggatcgct
     4321 tccggttcac caccgtccac caacagttgc aggcccttgc gtgcaaaagc gtcggattcc
     4381 tcctcagtaa ttgcttccag gcccagcaaa ccttccttgc gcgcggtcat gctccagttg
     4441 atgaccttgc cgatgttgtc gcgcatgttg accactggcg gaaacaccac ccagcgcagc
     4501 acgacgaagg catggcgaaa cgtcaccatg ggcgtctgca gcatggcggc gccaaaagta
     4561 ccgccgataa cgatcacggc tgcgggcgca ttggccaggc cggaaatatg cccgccctcc
     4621 atcgcattgc caccaatgat ggcaacgatg ccgacgagaa taccaatcag gctcagaata
     4681 tccatggtgt cagcgcaact cgctcaggta ttttccaaga tccttcaaat ccacaacggc
     4741 gtcggacaag cctgcagttt ccaccgccat cggcatgcca tacaccacac aactctgttc
     4801 gttctgggtc cagacggtag cgccgccacg tttcagcatc ttgcagcctt cacgaccgtc
     4861 gtgtcccatg ccggtaagaa ctgctgcaag aatcttgcct ttcacactgc gggcagccga
     4921 accgaaggtg acatcgacgc tgggccggta ctggatcttc tcgttgccct ccaccaccga
     4981 caccgcctgg ccgcgtgcat ccagcaacat ctgcttgccg ccgggtgcca gcagggccca
     5041 gcccggacgt accggatcat tgtcttcagc ttctttcacg cggattttgc acaaacggtc
     5101 gagccgttcc gcaaacgccg tggtaaaggt gccaggcatg tgctgcacca gcaaaatcgg
     5161 g
//
LOCUS       DASUEG010000281         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_468819, whole genome shotgun sequence.
ACCESSION   DASUEG010000281 DASUEG010000000
VERSION     DASUEG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468819"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFX11_05220"
     CDS             complement(<1..438)
                     /locus_tag="VFX11_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX4938051.1"
                     /translation="MSKPLFDPADTNLAQLFMRAFYWADEGLQNALKKKGWPAITRAQ
                     SLVFVNIGEGVTRPSDIASRVGVTRQAIHQTINELVDMGFLKLEPDPRDRRAKVVVFT
                     ETGAKIGGDAVDALRQIEQSLSSRIGGERVQALREALTLEWGDP"
     gene            complement(453..1370)
                     /locus_tag="VFX11_05225"
     CDS             complement(453..1370)
                     /locus_tag="VFX11_05225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008008641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4938052.1"
                     /translation="MLSFLPGPIKGALAFLLMATNTVLCVVPLLLITLFKLLLPLAFW
                     QRWTAAVLIVIAETWIANNNAILALTQKLELDVRGLDGLHYHGWYFVTSNHQSWSDIL
                     ILQKIFNRKIPFLKFFLKQELIWVPLMGAAWWALDFPFMKRHSKEFLEKHPEMKGKDL
                     ETTRKACEKFRELPVSVMNFLEGTRYTKQKHDQQQSPYNYLLKPKSGGIAFVLSAMGG
                     QLRSLLDVTIVYHDKANGFWDLMCGRVRRISVRVVERSIPAEFAQGDYEEDAAFRARF
                     QSWVNEIWQEKDVLIAAMKQEAAASVRLR"
     gene            1731..3164
                     /locus_tag="VFX11_05230"
     CDS             1731..3164
                     /locus_tag="VFX11_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938053.1"
                     /translation="MAQSKTTPTLLLASLLLLPGIAMADSFEDAVNEYLKGFKECTEA
                     NTLRTKDLNGAKKKFDTYLKTLDKATAMDSSILSTTQRDMDKNLRYCERVELDIKRAE
                     ATPILEKGFTYCDAAKTALDSGDVATAQTNLDEYKRYRDDAFIITETIMEVFAMASKV
                     RACSRVEEKLAEASLKESAVAAAMTKAATDYQSFLNECTGAKTAITSPKFSLNSLDAV
                     NQQLNNAMKFKKAARENGDAFATMSAQPERPESQSLKKLVDSATACEGEVSGYIRTAT
                     KNKRGIEKEILDGTAVLKQSMAACESSIKSSERFTDETDLTRAEADFNSSASLKRKIT
                     SNSKLIASAQQYPDWKASQEFSQLMNSTESCQTRAAASIKNQKSALATRQQKAKDDAA
                     RQAKLEEERKRLAEEQARKDAEAVKQKQQAEAAAKKAEEQAKKEAAEAAKFEDVDVGD
                     IDEDLGSLEADKDKEKGSGKSWTDLVK"
     gene            complement(3329..>3442)
                     /locus_tag="VFX11_05235"
     CDS             complement(3329..>3442)
                     /locus_tag="VFX11_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4938054.1"
                     /translation="ARFYFKRILPRTKSLVETMNSGVNSLMDMEVDNFGFY"
BASE COUNT          908 a          998 c          893 g          643 t
ORIGIN      
        1 cggatcaccc cactccaggg tcagcgcttc gcgcaacgcc tgcacacgct caccaccaat
       61 gcggctcgac agcgactgct cgatctgccg caacgcatcc acagcgtcac caccgatttt
      121 ggcgccggtt tcggtgaata ccaccacttt ggcccgccgg tcgcggggat cgggttccag
      181 cttcagaaac cccatatcca ccagctcatt gatggtctgg tggatcgcct gacgggttac
      241 gccgacgcgg gacgcaatgt cagacggccg ggtaacgcct tcgccgatat tcacgaacac
      301 cagcgactgc gcccgcgtaa tggcaggcca gccttttttc ttcaacgcat tctgcaggcc
      361 ctcatcagcc caatagaacg cgcgcatgaa caactgagcc agattggtat cagccggatc
      421 gaacaatggt ttggacatac ttcatgaacc tgtcaacgta accgaacgga agcggccgct
      481 tcctgtttca tcgcagcaat cagcacgtct ttttcctgcc agatttcgtt cacccaggac
      541 tggaagcgcg cgcgaaaagc agcgtcttcc tcataatcgc cctgggcaaa ttccgccggg
      601 atggaacgtt ccacaacacg tactgaaatg cgacggacac ggccgcacat caaatcccaa
      661 aagccattgg ctttgtcatg gtacacaatc gtcacgtcca gcaatgaacg caactgcccc
      721 cccatagcgg ataacacgaa cgcaataccg cccgatttcg gtttaagtaa atagttgtac
      781 ggcgattgct gttgatcgtg tttttgtttt gtataacggg ttccttccag aaaattcatc
      841 accgagacgg gaagctcgcg gaatttctcg cacgccttgc gcgtggtttc cagatccttg
      901 cctttcatct ccggatgttt ttccagaaat tccttcgagt gccgtttcat gaacgggaaa
      961 tccagcgccc accaggccgc ccccatcagc ggcacccaga tcagttcctg cttgaggaaa
     1021 aatttcagga acggaatctt gcgattgaaa atcttctgca ggatcaggat gtcgctccag
     1081 ctctggtgat tgctggtgac aaaataccag ccgtggtaat gcaggccatc cagcccgcgc
     1141 acatccagtt ccagcttctg ggtcagcgcc agaatcgcgt tgttgttggc gatccaggtt
     1201 tcggcgatca cgatcagcac ggctgcagtc cagcgctgcc agaatgccag cggcagaagc
     1261 agtttgaaca gggtaatcaa cagcaacggc accacgcaca gcacggtatt ggtggccatc
     1321 agcagaaacg ccagagcacc cttgatgggc ccgggcagaa acgacagcat gcagagactc
     1381 ccaagggtaa acgggcgaga cacccagttc tgttcgtaaa gtggccgcac attatgccac
     1441 aagcccctgc cggcgaaacc gtaaaccgta cacccgtacg aggatttgtt ggaccgatca
     1501 gtcgctcctt tattgcgggc ggcgcggaga aacctgctga atcgttggcc aaactgaaaa
     1561 agtttcctat aaagagaccc aaagttgatc tatatttaat agccagggag caaaaaccag
     1621 ttaattcatt ggtttagatg gatttttagg tagttcttat acgaatctgt cgataagcgg
     1681 ctacttactt atttcgacct gcacaatcat tgaacatagg tgcacaacga atggcgcaat
     1741 ccaagacaac cccaaccctg ttgctggcgt cccttctgct cctgccgggt atcgccatgg
     1801 cagacagttt cgaagacgct gtgaacgagt atctgaaagg gttcaaggaa tgcacggaag
     1861 ccaataccct gcgcaccaag gatttgaatg gcgccaagaa aaagttcgat acctatctga
     1921 aaacgctcga caaagccacg gcaatggaca gcagcattct gagcaccacc cagcgcgaca
     1981 tggacaagaa cctgcgctat tgcgaacggg tcgaactgga tatcaagcgc gccgaggcaa
     2041 ccccgatcct ggaaaaaggc ttcacctact gtgacgctgc caagaccgcg ctggacagcg
     2101 gcgatgtcgc cactgcccag accaacctgg atgaatacaa gcgctatcgc gatgacgcct
     2161 tcatcatcac cgaaaccatc atggaagtct tcgccatggc atccaaggtg cgggcctgtt
     2221 cgcgggtgga agaaaaactg gcagaagcct ccctcaagga aagcgccgta gccgccgcca
     2281 tgaccaaggc cgctaccgac taccaaagtt tcctgaatga atgcaccggc gccaagaccg
     2341 ccatcacctc gcccaagttc tcgctcaaca gcctggatgc cgtcaaccag cagctgaata
     2401 acgccatgaa attcaagaag gccgcgcggg agaatgggga tgcgtttgcc accatgtccg
     2461 cccagccgga acggcctgaa agccagagcc tgaaaaagct ggtggacagc gccaccgcct
     2521 gtgaaggcga agtgtccggt tatatccgca cggcgaccaa gaacaagcgt ggcatcgaga
     2581 aagagattct ggacggtacc gccgtattga aacagtcgat ggccgcctgc gaatcttcca
     2641 tcaaatccag cgagcgtttc accgacgaaa ccgacctgac gcgtgctgaa gcagacttca
     2701 acagttccgc atctctgaag cgcaaaatca cctccaacag caaactgatc gcatccgcgc
     2761 aacagtatcc cgactggaaa gcgagccagg aattctcgca gctgatgaat tcgactgaat
     2821 cctgtcagac ccgcgctgcc gccagcatca agaaccagaa gtccgcgctg gccacccgcc
     2881 agcaaaaggc aaaagacgat gcggcacgcc aggccaagct ggaagaagag cgcaaacgtc
     2941 tggcggagga acaggcacgc aaggatgccg aagccgtcaa acagaaacag caggccgaag
     3001 cagccgcaaa aaaagcagaa gaacaggcca agaaggaagc cgcggaagcc gccaagtttg
     3061 aagatgtcga cgtgggcgac atcgatgaag atctgggctc gctggaagcc gacaaggaca
     3121 aggaaaaagg cagcggcaaa tcctggactg atctggtcaa atagaccgat cggagcagaa
     3181 aagcagggcg gcggttgcaa caaacccgcc gcctttttta tggcagtctg tcagccggct
     3241 cacctgacat tttgttcctg ccagcaaacg caggacaaaa aaaagcccgc ggttggcggg
     3301 caaaggacta cagagggaac cgtttcaatc aatagaagcc gaaattgtcg acttccatat
     3361 ccatcaagct gttgacgccg ctgttcatgg tttccaccag tgacttggtg cgcggcaaaa
     3421 tgcgcttgaa gtagaatcgt gc
//
LOCUS       DASUEG010000282         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2119614, whole genome shotgun sequence.
ACCESSION   DASUEG010000282 DASUEG010000000
VERSION     DASUEG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119614"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..2579
                     /locus_tag="VFX11_05240"
     CDS             186..2579
                     /locus_tag="VFX11_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4938055.1"
                     /translation="MRNQKGKMLRVCALLACVLAGEVAAARAAPDAPGVFREPAPAVT
                     RLLTALPPPKPLVHARSGQVALLQYEQLISQARLTAPRLGLAGFRLDPVTLTTGIEPR
                     ILQVEILSTSAEKQTPRLWQAGGESRFEHVQFAPDGRTLAALRVQTGQPSRLVLYDIA
                     TGSERSLDAGVNPAWGNPCAWVGNDRLLCRMVPAGHKPPAAPALAPLVMSHAGAPTPV
                     RTYDNLLESPSDDDRFDQYFTTELALITRDGREQRLAVAPGPIRSFLPSPDGSLVILR
                     RLERPYSRLVPASGFPARIEAWNLQTGQRIYQSALMGFGIEEEDGEGADAETLVWAPL
                     LPLTAGFLFQQQEGGKLRYQWRSVQAPFKGENTKVVAASDFPIRRFGWTSAGTPWYAC
                     DTAVRGELEVRVVLADGSARGLWRGTAADRYGSPGRAIRLDGSTGPVLESSGEIFLEG
                     DGLAATGPQPFLDALNLQTGATRRLFLARAGEFDEVLAVLDATGSRLLTSRESETSPP
                     TLYLLENGKARLLRQQPDPYPELQAVERRLITYPRADGVKLAGTLYLPKQRAKNKPLP
                     TLIWIYPYEYNDPQQAEQLDERQFRFHRIKGPAAIAAAVAGYAVLVNPTVPIIQEGSE
                     PADEYLPQLVASVEAAVDYLAKEGISDPSRMAVGGRSYGAFSSANLLIHSQRFATAIA
                     MSGAYNRTLTPFGFQHEKRTFWDATEYYTSISPFFHANRIKVPLLLVHGGSDPNPGTP
                     TLQARRFFHALAGEGATVRYVELPFEEHHYKGRETVLHAAWEMIDWLDTTIGPAVPR"
     gene            complement(2589..3848)
                     /locus_tag="VFX11_05245"
     CDS             complement(2589..3848)
                     /locus_tag="VFX11_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4938056.1"
                     /translation="MNQASVVPVHMGTLRRLTLSLPYFRDWSVPELKAIEPCCRSVVA
                     SAPTTLLKLNSVEPFVYFLIRGEVELQDANGESRILQAGDLDAGFPIANQRPCPFQVN
                     ALAGAELLRIEASKLRCHQVSRKPVRFFAEEEAVNAPWRSHPLVERLIRQAQEGTLAL
                     PAMPGIALRVRRALTKSDVRLDDIASIINADPAIVARLLKVANSVLFGAQSHCETVKS
                     ALLRLGMEKSQSLVTSLLARDLFSARNATVKNLMLQRWRHAIDMAALCAVLARMTPGL
                     QSERAMLVGLLHEIGALPLIRMTEAHPDLLGDPATLRSILTHLTPELSAMVLEEWGFD
                     TDFITAAQHQNHWFRDHDGKADYTDVLLIAHLHGQVHERAQWHLPRIDEVPAFAKLAL
                     GQLTPDLSLKVLDEARQQIQELKSLLA"
BASE COUNT          681 a         1272 c         1266 g          708 t
ORIGIN      
        1 cccatgcgtt atgcccccta taaagacaaa gtgaaaatat tcaaattcct gttcaagtga
       61 cagcgactgg caacccggtg aacgcataac ggccggcctt gtgccggccg ttgtccgcca
      121 tcaagattcc ggagcggtgg ggctgctaga gtggccgctg ccattcaaat ggaaggaatc
      181 gaggtatgcg aaatcaaaag ggcaagatgc tgcgcgtgtg cgcgctgttg gcatgtgttc
      241 tggccggtga ggttgccgcg gcccgggctg cgccggacgc tcctggcgtt ttccgggagc
      301 ctgcccccgc cgtcacccgc ctgctgaccg cgttgccgcc acccaaaccc ctggtgcatg
      361 cccgttccgg ccaggtggcc ttgctgcagt atgagcagct catcagccag gcccggctga
      421 cggcgccccg gctggggctg gcaggtttcc ggctggaccc ggtgaccctc accacgggca
      481 tcgagccccg gattctgcag gttgagatcc tgtccacgtc cgctgaaaag cagaccccgc
      541 gactctggca ggcgggcggt gaaagccgct tcgagcatgt gcagttcgcc ccggacggcc
      601 gcaccctggc cgccctgcgc gtgcagacgg gccagccgtc ccggctggtg ttgtatgaca
      661 tcgccaccgg cagcgaacgc agcctcgatg cgggggtcaa tccggcctgg ggtaatccct
      721 gcgcctgggt gggcaatgac cggctgctgt gccggatggt gccggccggc cacaagcccc
      781 cggcagcgcc ggcactggcg ccactggtga tgtcccacgc cggagccccc accccggtgc
      841 gcacctatga caatctgctg gaaagcccct ccgacgacga ccgtttcgat cagtacttca
      901 caaccgaact ggcgctgatc acccgtgatg gccgcgagca gcgtctggcg gtcgcccccg
      961 gcccgatccg cagtttcttg ccatcgcccg atggcagcct ggtgatcctg cgccggctgg
     1021 aacgccccta ttcccgcctg gtgccggcca gcggcttccc tgcccgcatc gaggcctgga
     1081 acctgcaaac cgggcagcgc atctaccagt ccgcgctgat gggtttcggt atcgaggaag
     1141 aggatggcga gggtgctgat gccgaaaccc tggtctgggc gccactcctg cccctgacgg
     1201 ccggcttcct gttccagcaa caggaaggtg gcaagctgcg ctaccagtgg cgctcagtgc
     1261 aggcgccttt caagggcgag aacacgaaag tcgtggccgc cagcgatttt cccatccgcc
     1321 gtttcggctg gaccagcgcc ggcacgccct ggtacgcctg tgataccgcc gtgcggggtg
     1381 agctggaggt gcgggtggtg ctggcggacg gcagtgcccg cggcctgtgg cggggcacgg
     1441 cggccgaccg ctacggcagt ccggggcgcg caatacggct ggatggcagc acgggccccg
     1501 tgctggaatc cagcggcgag attttcctgg agggggatgg tctggctgcc acgggtccgc
     1561 aaccgttcct ggacgccctg aacctgcaga cgggtgccac ccgccgtctg ttcctggcca
     1621 gggccggcga attcgatgaa gtgctggcgg tactggatgc aaccggttca cgcctgctca
     1681 cctcgcggga atcggaaacc agccccccca ccctgtatct gttggaaaac ggcaaggcca
     1741 ggttactgcg gcaacaaccc gatccctatc ctgaattgca agcggtcgag cgccggctga
     1801 tcacctatcc ccgtgccgac ggcgtgaagc tggcgggtac cctgtatctg ccgaagcagc
     1861 gtgcgaagaa taaacccctg cccacgctga tctggatcta tccctacgaa tacaatgatc
     1921 cgcagcaggc cgagcagctt gacgagcgcc agttccgctt ccaccgcatc aaggggccgg
     1981 cggccatcgc ggcggccgtg gcgggttatg cggtgctggt gaatcccacg gtgcccatca
     2041 ttcaggaagg ctccgaaccg gcggacgaat acctgccgca actggtggcc agcgtcgagg
     2101 cagcggtgga ttacctcgcg aaagaaggca tcagcgatcc cagccgcatg gcggtgggcg
     2161 gtcgcagtta tggcgcgttt tcctctgcca acctgctgat ccattcccaa cgtttcgcca
     2221 ccgccatcgc catgagtggc gcctacaacc gcacgctgac accgttcggc ttccagcatg
     2281 aaaagcgcac cttctgggat gccaccgagt actacaccag catctcgccg ttcttccacg
     2341 ccaaccggat caaggtgccg ctgctgctgg tgcacggcgg ctccgatccc aatccgggta
     2401 cgcccaccct gcaggcgcgg cgctttttcc atgcgctcgc gggggaaggt gcgacggtgc
     2461 gttacgtgga attgccgttc gaggagcatc actacaaggg ccgcgagacc gtgctgcatg
     2521 cggcctggga aatgatcgac tggctggata ccaccatcgg cccggcggta ccgcgctgaa
     2581 ccggcggctc aggccagcag cgatttcagt tcctggatct gctgtcgggc ctcgtcgagc
     2641 actttcagac tgagatccgg agtgagctgg ccgagggcga gtttcgcaaa ggcgggcact
     2701 tcgtcgatgc ggggcaggtg ccattgcgcg cgctcatgca cctgcccgtg cagatgcgcg
     2761 atcagcaata cgtcggtgta atcggccttg ccgtcgtgat cgcggaacca gtggttctgg
     2821 tgctgggcgg cggtgatgaa atcggtgtcg aatccccact cttccagcac catggccgac
     2881 agttccggcg tcagatgggt gagaatgctg cgcaacgtgg ccggatcgcc cagcaggtcg
     2941 ggatgggctt ccgtcatccg gatcagcggc agggcgccga tctcgtgcag cagccccacc
     3001 agcatggcgc gttcggactg cagccccggg gtcattcgtg ccagcaccgc gcacagggcc
     3061 gccatgtcga tggcatgacg ccagcgttgc agcatcaggt ttttcacggt ggcattgcgg
     3121 gcagaaaaca gatcccgcgc cagcaggctg gtgaccagac tctgggattt ttccatgccc
     3181 agccgcagca gggcggattt caccgtttcg caatgggatt gcgcgccgaa cagtacgctg
     3241 ttggccacct tgagcagccg cgccacaatg gccgggtcgg cattgatgat ggacgcgatg
     3301 tcgtcgaggc ggacgtcgga tttggtgagc gcccgccgca cccgcaatgc gatgccgggc
     3361 atggccggca gtgccagcgt cccttcctgc gcctgacgga tcaggcgttc caccagggga
     3421 tgtgagcgcc agggcgcgtt caccgcttct tcttcggcga aaaaccgcac cggtttgcga
     3481 ctgacctggt gacagcgcag tttcgaggct tcgatccgca gcaattccgc tcccgccagc
     3541 gcattcacct gaaacgggca gggtcgctgg ttggcgatgg ggaagccggc atccaggtcg
     3601 ccggcctgca gtatccgtga ttccccgttc gcatcctgca attccacttc gccccgaatc
     3661 aggaaataga caaacggctc cacactgttg agtttcagca gtgtggtggg cgcgctggcc
     3721 accacggagc ggcagcaggg ttcaatggcc ttcagctccg gcacgctcca gtcgcgaaaa
     3781 tagggcaacg agagcgtaag ccggcgcagt gtgcccatgt ggacgggcac aacggaagcc
     3841 tggttcatcg acaaaccttc ctttgcgggc aaatcgtttc aacgcttttc ttggggggac
     3901 tctgcctgaa atcaggctgc ttgtttc
//
LOCUS       DASUEG010000283         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1793612, whole genome shotgun sequence.
ACCESSION   DASUEG010000283 DASUEG010000000
VERSION     DASUEG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793612"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFX11_05250"
     CDS             <1..713
                     /locus_tag="VFX11_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938057.1"
                     /translation="DLSLPGRFRRQADYMDQHPDVGVVGCHSRCVYSGGELPPYIEGK
                     SFTGDASRRMITGNLFHQGEVMMRRDCYEKVGGYRTFFKFAQDRDLFCRLSQVTHFHV
                     IEEVLYHRYVGVPGSVSGSASKLIVQRFLSDFACYCHGQRLAGKRDPLDEHGARAALF
                     WAPSQRIRQEVFHRAIRALYFKRDADFRIYRDALLQKDTHLARRLFFTLAEKFPGLTS
                     LLVDAYYKRKLSDSETSA"
     gene            755..2041
                     /locus_tag="VFX11_05255"
     CDS             755..2041
                     /locus_tag="VFX11_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938058.1"
                     /translation="MYQPLLKTGFQALAGIVLLLASATTVRAELTLPLQTMAQSARLY
                     YMSPTGLGSKDGSSEDNAFAWPQLASIASDFDAASRIVLLPGTYDLTGTVDLRSPTPD
                     TVLVLEGRDGALIKGNFDFATGTGTGSGLRLRTGNLILRGLSIQNTGFCVKSEKASVV
                     NQVLIENLTAKNVHTCILVDRDSTQPVTRWIVRDSRIQGYYRAAIRLAGAKSSDFLLE
                     KLQIDGAHDQAKSDCYKAGIQLYAGVSQVHIRDSSVLNNIGSCGEAYQQGDGIEADHK
                     EGTPDQILLENLEVGNSGDADLDLKADHVTLTKVVTQGGPLTRYAFKVWTYDNYQCNH
                     CYAYGPNKAYINLNQATMRFTGSTFANDLPVHICDLRHGTTPEEQAVVQFVDTSIYIG
                     NDEWLNECGPGVLAGVQRLPPGKLAPPEPVTNLRGR"
     gene            2091..>3121
                     /locus_tag="VFX11_05260"
     CDS             2091..>3121
                     /locus_tag="VFX11_05260"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4938059.1"
                     /translation="MQKRTLLTLLLACGALAGTTLTGTARADLLDPAPVKLGGVDLVP
                     QLKLQEEYDDNFYSQVNTQEFWVQILTLQLDALAPVGPHEYSAHYKTEAGVVESSSED
                     NYVDQSIYLRGFWDPDPRHRFELKGGYRQDHERRGTENFQGSKAFTIDETPEFEESSV
                     LGRYTYGADKSRGRLVLEARGSDKTYMNLRELTERSDRQNMEGTATFYWRVAGSLHGL
                     LEANQGEYDYVNDLEPRPGAVDTGDNDYTHLLAGVTWEAAGKTDGTLKVGRAEKNFTD
                     ADREDFAGNSWYGEIKWKPRTYSTFRLNTASRTEEPPTGRGDYVDVTEWGIDWRHAWS
                     DRIASRVRYL"
BASE COUNT          673 a          994 c          931 g          523 t
ORIGIN      
        1 gcgatctgtc gctgccgggc cggttccgca ggcaggccga ttacatggac cagcatcccg
       61 acgtgggcgt ggtgggttgt cactcccggt gtgtatacag cggcggcgag ttgccgcctt
      121 atatcgaggg caaatccttc accggcgacg ccagccgccg catgatcacc ggcaacctgt
      181 tccaccaggg cgaggtgatg atgcgacggg actgctatga aaaagtcggc ggctatcgca
      241 ccttcttcaa gttcgcccag gatcgcgacc tgttctgccg gctgagccag gtaacccatt
      301 ttcatgtgat tgaagaagtg ctctatcacc gttatgtggg cgtgcccggc agcgtcagcg
      361 gcagtgccag caagttgatc gtgcagcgct tcctgtcgga ttttgcctgc tattgccacg
      421 gccagcgcct ggcgggcaag cgcgatccgc tcgacgaaca cggcgcgcgg gccgccctgt
      481 tctgggcccc gagccagcgc atccgccagg aagtattcca tcgcgccatc cgcgcgctgt
      541 atttcaagcg cgacgccgac ttccggattt accgcgacgc gctgctgcag aaagacaccc
      601 atctggcgcg gcgactgttt tttacgttgg cagaaaaatt tccaggcctg accagcctgc
      661 tcgtggacgc ctattacaag cgcaaactgt ccgactcgga gacgtccgcc taaggcggcc
      721 tatcgggaac acccaaggga agaggaaacc gatcatgtat cagcctctgt tgaaaaccgg
      781 gtttcaggca ctggccggca tcgtattgct gctggccagc gcgaccaccg tgcgcgccga
      841 actcaccctg ccgctgcaga ccatggccca gtccgcccgg ctgtattaca tgagccccac
      901 gggcctgggc agcaaggatg gcagcagcga ggacaacgcc ttcgcctggc cacaactggc
      961 gagcatcgca tccgatttcg acgcggcttc ccgcatcgtg ctgctgccgg gcacctacga
     1021 cctgacgggt accgtcgacc tgcgctcgcc cacccccgac accgtgctgg tgctggaagg
     1081 tcgcgacggc gcattgatca agggcaactt cgatttcgcc accggaacgg gtacgggttc
     1141 cggcctgcgc ctgcgcactg gcaacctgat cctgcgcggc ctgtccatcc agaacaccgg
     1201 tttttgcgtc aagtcggaaa aggcatcggt ggtgaaccag gtactgattg aaaacctgac
     1261 cgcgaagaac gtgcacacct gcatcctggt ggatcgcgat tccacccagc ccgtcacgcg
     1321 ctggatcgtg cgggattccc gtatccaggg atattaccgg gcggcgatcc gcctggccgg
     1381 cgcaaaatca tcggatttcc tgctggaaaa actgcagatc gacggcgccc acgaccaggc
     1441 caaaagcgac tgctacaaag cgggcatcca gttgtacgcg ggggtgagcc aggtccacat
     1501 ccgcgacagc tcggtgctca acaacattgg ctcctgcggc gaggcttacc agcagggcga
     1561 tggcatcgag gccgatcaca aggaaggcac accggaccag atcctgctgg agaacctcga
     1621 ggtcggcaac agtggcgatg ccgacctgga tctcaaggcc gaccacgtca ccctgaccaa
     1681 ggtagtgacc cagggcgggc cattgacccg ctatgcattc aaggtctgga cctacgacaa
     1741 ctaccagtgc aaccactgct acgcctacgg accgaacaag gcctacatca acctcaacca
     1801 ggccaccatg cgctttaccg gttccacctt cgccaacgac ctgcctgtgc atatctgcga
     1861 tctgcgccac ggcaccacgc ccgaagaaca ggcggtagtg cagttcgtgg ataccagcat
     1921 ctacatcggc aacgacgaat ggctgaacga gtgcggccca ggcgtgctgg cgggagtgca
     1981 gcggctgccg ccgggaaagc tggcgccacc ggaacccgtg accaacctgc gggggcggtg
     2041 aaaccggact gaacatgcct ttccaaacaa tatcaggaca gcagaaacag atgcagaaac
     2101 ggacattgct cacattgtta ctcgcctgcg gcgcacttgc gggcaccacc ctgaccggca
     2161 cggccagggc cgacctgctg gacccggcgc cagtcaaact cggcggcgtg gatctggtac
     2221 cccagctgaa gctgcaggaa gaatacgacg acaacttcta cagccaggtg aatacccagg
     2281 aattctgggt gcagatcctc actctgcagc tggatgcgct ggcgccggtc ggcccccacg
     2341 aatattcggc gcattacaag actgaggcgg gcgtcgtcga aagcagcagc gaagacaatt
     2401 atgtggacca gtccatttac ctgcgtggat tctgggatcc cgatccccgc caccgctttg
     2461 aactcaaagg cggctaccgg caggatcacg aacgtcgcgg taccgaaaac ttccagggca
     2521 gcaaggcgtt caccatcgac gaaacgccgg agttcgaaga atcctccgtg ctggggcgct
     2581 acacctacgg cgcggacaag tcccggggcc ggctggtgct ggaagccaga ggcagcgaca
     2641 aaacctacat gaacctgcgc gaactgaccg aacgcagcga ccgccagaac atggaaggca
     2701 ccgccacctt ttactggcgg gtggcaggca gcctgcatgg cctgctggaa gccaaccagg
     2761 gcgaatacga ttacgtcaat gatctggagc cccggcccgg cgccgttgat acgggcgaca
     2821 acgactacac gcatctgctg gccggtgtga cctgggaggc cgccggcaag accgacggca
     2881 cgctgaaagt aggccgggcg gaaaagaatt tcaccgacgc cgaccgcgag gatttcgccg
     2941 gcaacagctg gtacggggaa atcaagtgga aacccagaac ctattccacc ttcagactca
     3001 ataccgccag ccgtaccgag gaacccccta ctggacgggg cgattatgtc gacgtgacgg
     3061 aatggggcat cgattggcgc catgcctgga gtgatagaat tgccagccgc gtccgctacc
     3121 t
//
LOCUS       DASUEG010000284         3711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1127509, whole genome shotgun sequence.
ACCESSION   DASUEG010000284 DASUEG010000000
VERSION     DASUEG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127509"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..2097
                     /locus_tag="VFX11_05265"
     CDS             451..2097
                     /locus_tag="VFX11_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4938060.1"
                     /translation="MKKINRRNFLGGAAVTAAGAAMPDAAMAVGRIRVTRSEERAVII
                     GSGFGGGASALRLAQAGVKVLVLERGRWWNTGPNSTTFPRAMVPSKKDFFYTTWPEFG
                     GVRIGLFPYVGLLEATVGINHTAIAACGVGGGSLVYQGMTLQPTEENFSAWFPAAVDY
                     AEMDSVFYPRVAQMLKVQTAPDELINSPSYLAARIFGKRVLEAGYDLTKIPMPIDWQF
                     ALDELKGLMAPSYTNGDCAFGVRNGGKHSVDVTYLKEAIATGNVTVASQHNVRRIARE
                     ADGSWSVYADRTSSTGTVLEHKIIKTKALIVAAGTSNTNKLLLAAGGRGDITDLPDEL
                     GQGYGTNADQIYVWDRMPENPGAPQGGPVIYGSREWNDPNQLPNTIIQASLPPIYKKD
                     KSAVGISTALIPGAAKLPRRAAEWYATMLVGFGISPDRGKFIYNPERDRVELHWPKNG
                     DAAVAARIHERIAAVAGPESALINTNKLVNTTWHPLGGACIDVVCDLEGRVKGQKGLY
                     VLDGALMPGTTCACNPSMTIAAVVERALDRIVKNDVGTII"
     gene            complement(2168..3028)
                     /locus_tag="VFX11_05270"
     CDS             complement(2168..3028)
                     /locus_tag="VFX11_05270"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX4938061.1"
                     /translation="MHFDLEQLLKHLRVQSVGDALYRGENLPGKRRAVFGGQVIAQAL
                     SAAIQHLNSDRQPHSLHCHFLRPGNVAVSIDYQVTVVRDGGSFSLRQVTALQEGKAIF
                     IATISFQKPEGGLQHQQPAPPMQPRNEMISEWEFWQLVQQERPEITYLRPPNFTALDI
                     YSRFRPGVNAPAPQADPHQAFWFRANGKMTEPADHFLIVAFQSDLLFLNTAFHAHPYT
                     LIDPQVQAASLDHCLWFYADINASEWLYYDMRSPVSGGGRGLNHGFFYTEAGTLVAST
                     AQEGLMRIKQ"
     gene            complement(3075..3638)
                     /locus_tag="VFX11_05275"
     CDS             complement(3075..3638)
                     /locus_tag="VFX11_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4938062.1"
                     /translation="MPDWTWCYGRADPNPSRLFSDTAAAFADSISALGTQAAYCGICV
                     VDDAGVQQVCDELIPAMAPGSHIAIHSTVHPALCRALAVQARAHGLWLVDAPVSGGGR
                     GAARGDLTVMVGIAREAFTRLVNVSSGRSFSFDVRARMASPGDFAHGAILLEKDLRLL
                     GEVLGDSPHYRSMRNVAADFLDLALNP"
BASE COUNT          814 a         1016 c         1135 g          746 t
ORIGIN      
        1 ctgttcggaa aagattgccg agccggcagg aagttgaaga ccgtcgtcaa caaagccagt
       61 tcggtttcca gcggtcgagg cgcaaacaac cgcactctca gccaccgtta cctgaatgcg
      121 aagcgattga aatcatttgc agtgccttga tggcaaccct ctggaaggcc gcgaaaagta
      181 cacaaagcat aggtccgcat ttttgcatga agcagtgaga acgcacgtgt gggcaactgg
      241 aaaaatgcca ggcggaaata tgcggatggt gtgcgtggcg cgctgccacg tattcaatat
      301 tttgaatact cgaaccgatc atcggtgaac atgcaggaaa ttcttgcata agttcaaact
      361 taaattgaag aatggttgcg tgagcgtggc ggactattgt tagatgccgc acacgcgatt
      421 ttcacaaaaa aacaagaagg ggtagttccg atgaaaaaaa tcaaccgacg taactttttg
      481 ggtggcgctg ctgttacggc tgctggtgcc gcaatgccgg atgcggcgat ggcggtgggg
      541 cgcattcgtg tgacacgttc agaagagcgt gcggtgatca taggatcggg ctttggcggc
      601 ggcgcgtcgg cgcttcggct ggctcaggcc ggggtcaagg tgctggtgct ggagcgtggg
      661 cgctggtgga acaccgggcc taactcgacg acatttccgc gtgcgatggt tcccagcaaa
      721 aaggatttct tttacaccac ctggcccgag tttggaggcg tgcgcatcgg tctcttcccc
      781 tacgtgggtt tgcttgaggc gacggtagga atcaatcata cagccattgc agcatgcggt
      841 gtgggtggcg ggtctctggt ctaccagggc atgaccttgc agcctaccga agagaatttc
      901 agcgcgtggt ttcccgcggc ggtcgactat gccgaaatgg attccgtctt ctatccgcgg
      961 gtagcgcaga tgctgaaggt gcagacagca ccggatgaat tgatcaacag cccctcctat
     1021 ctggccgccc gcatttttgg caagcgggtg ctggaagcgg gctacgacct taccaagatc
     1081 ccgatgccaa tcgactggca gttcgcgctg gatgaactga agggattgat ggcgccttcc
     1141 tacaccaacg gcgactgcgc atttggtgtg agaaacggcg gcaagcactc cgtcgatgtc
     1201 acctacctca aggaagccat tgcaaccggc aatgtcacgg ttgcgtcgca gcacaatgtg
     1261 cgcagaatcg cgcgcgaagc tgatggctcc tggtcggttt acgcggacag gaccagctcc
     1321 accggcacgg tgcttgagca caagatcatt aaaaccaagg ctctcattgt tgctgcaggt
     1381 acttcaaaca caaacaaact gttgttggct gcgggcggac gcggtgatat caccgatttg
     1441 ccggatgagc tcggccaggg ttatggcacc aacgccgacc agatttatgt ctgggacaga
     1501 atgccggaaa atccgggcgc gccgcagggc ggccccgtca tttacggcag ccgcgagtgg
     1561 aatgatccca accagcttcc caataccatc atccaggcat cgctgccgcc gatctacaaa
     1621 aaggataaaa gtgctgtggg aatatccaca gcactgattc ccggcgcggc caagctgccc
     1681 cgcagggcgg cggagtggta tgcaacgatg ctggtcggct ttggtatcag tcccgatcgc
     1741 ggcaaattca tctacaaccc ggaaagggac agggtagaac tgcattggcc gaagaacggt
     1801 gatgcggcag tggcggcccg cattcacgag cggattgcgg cagtggctgg tccggaaagc
     1861 gctttgatca acaccaacaa gctggtcaac actacctggc atccgcttgg tggcgcctgc
     1921 atcgacgtgg tgtgcgatct ggaagggcgt gtgaaggggc agaaaggact gtatgtcctc
     1981 gacggcgccc tgatgcccgg aaccacctgc gcctgcaacc cgtcgatgac gattgcggcg
     2041 gttgttgagc gtgcgctcga tcggatcgtg aagaacgacg tgggcacgat catctaggta
     2101 ccccgggtac ctgatccatc cgcaacaagt ccgcaaccat tgcccgtcgc agtggttgcg
     2161 gcttttttta ctgcttgatg cgcatcagtc cttcctgcgc agtgctggcg accagcgtgc
     2221 cagcctcggt atagaaaaag ccgtgattga gtccgcgtcc gccgccgctg accgggctgc
     2281 gcatgtcgta gtaaagccat tcgctggcat tgatatcggc atagaaccac aaacagtggt
     2341 cgaggctcgc ggcctgcacc tgtggatcga tcaaggtgta gggatgcgcg tgaaaggcag
     2401 tgttcagaaa cagcagatca gattgaaagg ccacgatgag gaaatggtcg gctggttctg
     2461 tcattttgcc gttggctctg aaccagaagg cctgatgcgg atcggcctgc ggcgctggcg
     2521 cattaacgcc aggacggaat cggctgtaaa tatccagcgc ggtgaagtta ggcggtcgca
     2581 gataggttat ctcgggccgt tcctgctgaa ccagctgcca gaattcccat tcactgatca
     2641 tttcatttcg gggctgcatg ggtggcgccg gttgctgatg ctgcagtccg ccttcaggtt
     2701 tctggaagga aatggtggca ataaagatcg ccttgccttc ctgcagtgca gttacctggc
     2761 gcagagaaaa actgccgccg tcccgcacca ccgtaacctg atagtcaatc gacaccgcaa
     2821 cattgcccgg gcgcaaaaaa tgacagtgca gtgagtgcgg ctgtcgatcg ctattcagat
     2881 gctgaatggc ggcgctcagg gcctgcgcaa ttacctggcc gccgaagacg gccctgcgct
     2941 tgccgggcag gttttcacca cggtaaaggg cgtcgccaac ggattgtacg cgcagatgtt
     3001 tcaatagctg ttcaagatca aaatgcatgg ttgtcggggg tttcgaaggg gatgcatagc
     3061 gtaccgggaa ttggttaagg gttcaaagcc aggtccagaa agtcggccgc cacattgcgc
     3121 atcgaccgat agtgcgggct gtcgcccagc acttcaccca agaggcgtaa atctttttcc
     3181 agcaagatgg cgccatgtgc gaagtcgcca ggggacgcca tcctggcgcg gacatcgaag
     3241 ctgaagctgc ggccactgct gacattcacc agccgggtga aggcttcgcg cgcaatgccg
     3301 accatgacag tcagatcacc ccgcgcagcg ccgcgtccac cgccgctgac cggtgcatct
     3361 accagccaca gcccgtgggc gcgcgcctgc acggccagtg ccctgcatag cgcgggatga
     3421 acggtggagt gaattgcgat gtgactgcct ggcgccattg ccggaatcag ttcgtcgcag
     3481 acctgttgca cgccggcatc atccacaaca cagatgccgc aataggccgc ctgagtgccg
     3541 agcgcgctga tgctgtcggc aaaggccgcc gctgtatcgg aaaaaaggcg cgagggattg
     3601 gggtcggcgc gcccataaca ccacgtccag tccggcatcg atcatgcgcc gggccatggg
     3661 gccgccctgg ctccccaggc cgataaagcc agccttactc atcgtaattt t
//
LOCUS       DASUEG010000285         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1894104, whole genome shotgun sequence.
ACCESSION   DASUEG010000285 DASUEG010000000
VERSION     DASUEG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894104"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..552
                     /locus_tag="VFX11_05280"
     CDS             253..552
                     /locus_tag="VFX11_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4938063.1"
                     /translation="MAIIGGAWTPNIIWYLSQAPRRFSELKYDLQGISAKVLAERLKR
                     LEQDGIVLRANVPTSPPTVEYRLSPLGLELKPAIEAIVSVGERLKQMKANPGVAV"
     gene            549..896
                     /locus_tag="VFX11_05285"
     CDS             549..896
                     /locus_tag="VFX11_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX4938064.1"
                     /translation="MSDEPKLTGRAKTLWNKLSAREKLAFLQRFREVDAGTLSQEDAC
                     RELEAIVAQFAREQRFSANRQKKNLSGVVDSASVCSFCGKGFTDADAMVKARSGAVIC
                     NQCLEKFRGSESS"
     gene            complement(1016..2251)
                     /locus_tag="VFX11_05290"
     CDS             complement(1016..2251)
                     /locus_tag="VFX11_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938065.1"
                     /translation="MPIASDFIKDIPITLQLVTLDDGEKLNIRALPAFLDSKNNGVTV
                     DLKTKIWAPEPNPAVPGALGSLYVEGSTPTLGHLTPDGKPFDFGASISSNVINQAFLA
                     AHEAGVTTMEIRPEIYPNATQEGIQVYAQGADFGEGAKIGMRIEPASAPYILFMPSTD
                     GAPGKFGWSDVNLYFDLYKPEWGEYRTLFGVTFDLEVPFDINTTDDGYLSIGIEQLPT
                     IHISKTASDGMLLIPPAFINGTLDYFMPEVMPRLAEKLKVVPLPRIYNHTLHMQQFWI
                     AGTGNNNLSLAGNLVPLAATAAATPPTTVVDYSAEDITVQQESVSSTGVVTSSSVKVA
                     NGEVTIDVDGINPNPTLGLLEYRYRVDGGGWSIWKYRETIKLSRLLAGVHTVEVCSRS
                     MLLKREQGCPVVQFETIVE"
BASE COUNT          544 a          685 c          809 g          566 t
ORIGIN      
        1 gaacggcccc tgcacgatgg gatagggcac acccaggcgg cgggtgaagg cgttggattt
       61 ccacatgacg gtactccttg tgcaatgcga tgtaggctgc gggaaattct atgcgcgcag
      121 cgccgggatg tcgttgcccc gggcaggatt ctgtggttac catccggtaa ctgatccgag
      181 tgctttgcca tgattccacg ccgcaaaaat tgtgttcccg cgccgccgga cgcctgtccc
      241 cttaccgagt gcatggccat catcggcggt gcctggacgc ccaatatcat ctggtacctg
      301 agccaggcgc cgcgccgctt cagcgaactg aagtatgatc tgcagggaat atccgccaag
      361 gtgctggcag agcgcctgaa acgcctggag caggacggta tcgtgctgcg ggccaacgtg
      421 cccacctcgc cgcccacggt ggaataccgg ttgtcgccgc tggggctgga actgaaaccg
      481 gcgatcgagg ccatcgtcag cgtgggcgag cggctgaagc aaatgaaagc gaacccagga
      541 gtggcagtat gagtgacgaa cccaaactca ccggccgggc aaagacattg tggaacaagc
      601 tgtcggcgcg ggaaaagctg gcttttctgc agcgttttcg cgaggtggat gccggcaccc
      661 tgtcgcagga ggatgcgtgc cgcgaactgg aagcgatcgt cgcccagttt gcccgcgaac
      721 agcgcttcag cgccaaccgg cagaagaaga acctgtcggg agttgtcgac agtgccagtg
      781 tctgctcgtt ctgtggcaag ggttttaccg acgcggatgc catggtgaaa gccaggtccg
      841 gtgcggtgat ctgcaatcag tgtctggaga aattccgcgg ttcggaatcg tcctgagatc
      901 gcggcggcgg gtcaacactg atcggctgca ggtgtgattc gctttttgct gaaggacttc
      961 cataaaaaaa ccggctgtgt tgccacagcc ggttttttgt tgagcctgcg cggggttatt
     1021 cgacgatggt ttcgaactgc accaccgggc aaccctgttc acgcttgagc agcatggaac
     1081 gggagcacac ttcaaccgta tgcacaccgg ccagcaggcg cgacagtttg atggtttcgc
     1141 ggtacttcca gatggaccag ccaccgccgt ccacccgata acgatactcc agcagaccga
     1201 gcgtcggatt cgggttgatg ccatctacat cgatagtgac ttcgccattc gcaaccttca
     1261 cggatgagct ggtcaccaca ccggtggaac tcacgctttc ctgctgcacc gtgatgtcct
     1321 cggcgctgta gtccaccacg gtggtgggcg gtgtggcagc ggcggttgct gccagcggaa
     1381 ccagattgcc tgccagcgac aggttgttgt tgcccgtgcc ggcaatccag aactgctgca
     1441 tgtgcagggt atggttgtag atgcgcggca gcggcaccac tttcagtttt tcggccagac
     1501 gcggcatcac ttccggcatg aagtaatcca gcgtgccgtt gatgaaggcc ggcggaatca
     1561 gcagcatgcc atcgctggcg gtcttcgaga tatggatagt gggcagctgt tcgataccga
     1621 tgctcaggta gccgtcatcg gtggtgttga tatcgaacgg cacttccaga tcgaaggtca
     1681 cgccgaacag ggtgcggtac tcgccccact caggcttgta cagatcgaag tacaggttca
     1741 cgtcggacca gccgaacttg ccgggggcac cgtcagtgct gggcatgaac aggatgtacg
     1801 gtgcgctggc cggttcgatg cgcatgccga tcttggcgcc ttctccgaag tcagcgccct
     1861 gggcatacac ctggatacct tcctgggtgg cgttcgggta gatctccgga cggatttcca
     1921 tggtggtcac gccggcttca tgggccgcca ggaacgcctg gttgatcacg ttggaggaga
     1981 tggaggcacc gaagtcgaac ggcttgccat cgggtgtcag gtgacccagg gtgggtgtgg
     2041 agccttccac gtagagcgag cccagggcgc cgggaacggc ggggttgggt tcgggtgccc
     2101 agattttggt tttcaggtcg acggtgaccc cattgttttt gctgtccagg aatgctggca
     2161 aggcacggat attgagtttc tcgccatcat ccagtgtcac gagctgaagc gtgatgggga
     2221 tatccttaat gaagtccgat gcgattggca taatgaggac atccagtatg ccagtaaata
     2281 gtggagcaaa aagatcaacg aggaaattga gcaggccatt gaatatatcc aggaagaagt
     2341 taccgagtag gcctgcatct agtatcgtaa tgtcgagatt gcggactttc gcttgagtat
     2401 cgctaagtga gagatccaag ttggcatttt ctctatcgat atctaacaag acatatgtcg
     2461 aaacatccaa ctggtcgatg ctgacggtgg tcacaaagtc gcgttttaag gtaaacaaga
     2521 taccccatat ttcgattgta gtggtcagct gcatgtcgac ttctttgcta gaaagattta
     2581 ctcgtatgga ctcgttgtct aata
//
LOCUS       DASUEG010000286         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_867015, whole genome shotgun sequence.
ACCESSION   DASUEG010000286 DASUEG010000000
VERSION     DASUEG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867015"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFX11_05295"
     CDS             complement(<1..765)
                     /locus_tag="VFX11_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4938066.1"
                     /translation="MTEPVLLATRHGATAWLQLNRPQAMNALSFDLVDALLPQLQALA
                     QDDSVRVLVITGSGKAFCAGADLKNLNEPKAPGEPDILDRVVELLDTLRTFPKPVIGA
                     VNGLALAGGMELMMCCDLIYAAESARIGDAHCNYGIVPGGGGAAIMPRLLPLPIAKYL
                     LFTGEFLPAQKLLQYGLLNDVVPDDQLSDTVATVAAVICQKSPIGLQRAKRVANEALD
                     KTAADALRDERLASRDQFRSWDYREGISAFLEKRKPD"
     gene            complement(762..1841)
                     /locus_tag="VFX11_05300"
     CDS             complement(762..1841)
                     /locus_tag="VFX11_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4938067.1"
                     /translation="MTIQAPLEQLNSPALTEPTSMHSWAVVIAQALEHAGVDSQALFA
                     QAGVEYKLAVNPYMRVPTAKMNQLFQLSVAATGDTCFGLRMEEFIHPTTFHALGYSLF
                     ASSTLQDYCERLVRFFRIASDNCRHHIEETPDEFKLRIDLLVPNLSFESQDGWSAALV
                     KFARSMYRPGFNPLRVDLRRPAPSAADQQRYRDYFRCPVQFGCHEWALHFCPDDMTAT
                     LPGANHELALINDRVVMEYLARLEKSDLVTRVRTHIVKLLASGNCCKEMVAARLNMSP
                     RNLQHKLDLLGTCYQDILDQTRQDLAIQYMANRHTSISEITYLLGFSDTSNFARAFKR
                     WTGMAPRDFRGTGHGAGPIAPVILS"
     gene            complement(1983..>3116)
                     /locus_tag="VFX11_05305"
     CDS             complement(1983..>3116)
                     /locus_tag="VFX11_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4938068.1"
                     /translation="PRPDTWYVGDSNRDMVTAKNAGITAIFYNGGCWDMEGLRRSFQN
                     DPQHAPDAIINSFDELMDLLESCQQRDTLAFAKIVSKARPHHFPGPKPPPVRVEPDWH
                     PSVVKLTPPKVILFDWHATLVDTLDAMYHAVDDMLPELREKGLLDRMVDPAQSKSPED
                     ARLVEYVRNYAQLHPKVKADRKISRTDIFEVLFGEDQEAKQIAHQIFNFHYRNHYGTV
                     LPFEPHVRQVLVSLRMLNFKLGVITNRDREFFEHEVNAVENGSWQGLFDCMICGDDTV
                     LRKPHPDQVLLAAAQLGCEPAREVWYVGDSTTDVIASKTANITSVFFNGAQWDQPWLN
                     KIFPGNERYPHKPDVVVNDFSEFWALVLACLKRGYGESVLSGV"
BASE COUNT          569 a          928 c          968 g          651 t
ORIGIN      
        1 atccggtttg cgcttttcca gaaatgcgct gatgccttcg cggtagtccc acgaccggaa
       61 ctggtcccgc gacgccaggc gttcatcccg cagcgcatcg gcggcggtct tgtccagtgc
      121 ttcgttggcc acacgcttgg cgcgctgcag gccgatggga cttttctggc agatcacggc
      181 agcgacggtg gccacggtgt cggatagctg gtcgtccggc accacgtcgt tcagcagccc
      241 gtattgcagc agcttttgcg ctggcagaaa ttcgccggtg aacagcaggt atttggcaat
      301 cggcagtggc agcaggcgcg gcatgatggc cgcaccgcca ccgccaggga cgatgccgta
      361 attgcagtgg gcgtcaccga tgcgtgcgct ttctgcggcg taaatcaggt cgcagcacat
      421 catcagttcc atgccgcccg ccagtgccag cccgttcaca gcgccgatca ccggcttggg
      481 aaatgtgcgc agggtgtcga gcagttccac cacgcggtcg agaatgtcgg gttcgcccgg
      541 cgctttgggt tcgttcaggt ttttcaggtc ggcgccggcg caaaacgcct tgccgctgcc
      601 ggtgatcacc agcacgcgca cgctgtcgtc ctgcgccagt gcctgcagtt gtggcagcag
      661 tgcgtccacc agatcgaaac tcagcgcgtt catcgcctgc ggtcggttca gttgcagcca
      721 ggcggtggcg ccgtggcgtg tggcaagcag cacaggttca gtcatgacag aatcaccggt
      781 gctatcgggc ctgcaccgtg gcccgttccg cgaaaatcgc gcggagccat gccggtccag
      841 cgtttgaacg cgcgggcaaa gttgctggtg tcggaaaaac ccagcagata agtgatttcg
      901 ctgatggagg tatgccggtt cgccatgtac tggatcgcca gatcctgccg ggtctggtcc
      961 agaatgtcct gatagcaggt gcccagcaga tccagtttgt gctgcaggtt gcgcgggctc
     1021 atgttcagcc tggccgccac catttccttg cagcagttgc ccgaagccag cagttttacg
     1081 atatgcgtac gcacgcgggt caccagatcg gatttttcca gtcgcgccag atactccatt
     1141 accacccggt cattgatcag cgccagttca tgattggcac ctggcagcgt ggctgtcatg
     1201 tcgtcggggc agaaatgcag tgcccactca tggcacccaa actgcaccgg gcagcggaaa
     1261 tagtcgcggt aacgttgctg gtccgccgcc gatggtgccg gccggcgcag atccacccgc
     1321 agtggattga agccaggccg gtacatggag cgggcgaatt tcaccagcgc cgcgctccag
     1381 ccatcctgcg attcaaagga aagattgggc accagcagat cgatgcgcag tttgaactcg
     1441 tcaggcgttt cctcgatatg atgccggcag ttgtcgctgg cgatgcggaa gaaccgcacc
     1501 agccgttcgc aataatcctg cagggtggag ctggcaaaca gcgagtagcc cagggcgtga
     1561 aatgtggtgg ggtggatgaa ttcttccatc cgcaggccga agcaggtgtc gccggtggcg
     1621 gccacgctca gctggaacag ctggttcatt ttcgccgtgg gcacccgcat gtagggattc
     1681 accgccagct tgtattcaac gccggcctgc gcaaacagcg cctgactgtc gacgccggca
     1741 tgctccagcg cctgcgcaat gaccaccgcc cagctgtgca tcgatgtggg ttcggtaagc
     1801 gcaggactgt tcagctgctc cagtggcgcc tgaatggtca tgggaagggc cttcttgttg
     1861 ttatgcgacg ggcatttgtg cgcgtccgac acggtgtcag gcggattccg ggcgcactac
     1921 cccctgcgca aaaggtaggg gatgggggag ggggttgtcc tagtccagtt ggacgatggc
     1981 ggtcaaaccc ccgacaacac gctctcgcca tatccccgct tcaggcacgc cagcaccagt
     2041 gcccagaatt cggaaaaatc atttaccacc acgtccggtt tgtgggggta gcgttcattg
     2101 ccggggaaaa ttttgttgag ccagggttgg tcccactgcg caccattgaa aaacacgctg
     2161 gtaatgttgg cggtcttgga ggcgatcacg tcggtggtgc tgtcgcccac gtaccacact
     2221 tcgcgtgccg gttcgcaacc cagttgggcg gcagccagca gcacctgatc cgggtgaggt
     2281 ttgcgcagta cggtgtcgtc gccgcagatc atgcagtcga acagcccctg ccacgatccg
     2341 ttttccactg cattcacttc gtgctcgaaa aattcccggt cccggttggt gatcacgccc
     2401 agtttgaaat tcagcatgcg caggctcacc agtacctggc gcacgtgcgg ttcgaacggc
     2461 agcacggtgc cgtaatgatt gcgataatgg aagttgaaaa tctggtgggc gatctgcttg
     2521 gcttcctgat cctcgccgaa cagcacctca aaaatgtccg tgcgcgaaat cttgcggtct
     2581 gccttgacct tggggtgcag ctgggcgtaa ttgcgcacgt actccaccag ccgtgcatct
     2641 tcgggggact tgctttgagc cgggtccacc atgcggtcca gcaaaccctt ctcccgcagt
     2701 tccggcagca tgtcgtccac cgcgtgatac atggcgtcca gcgtatccac cagggtggcg
     2761 tgccagtcga acaggatcac cttgggcggc gtcagtttta ccaccgatgg atgccagtcc
     2821 ggctccaccc gcacgggtgg cggtttgggg ccagggaagt ggtgcggtcg cgccttggac
     2881 acgatttttg cgaacgccag ggtgtcacgt tgctggcagc tttccagcag atccatcagt
     2941 tcgtcgaaac tgttgatgat ggcatccggc gcgtgctgcg gatcattctg gaacgaccgg
     3001 cgcagtcctt ccatgtccca gcagccgcca ttgtagaaaa tggcggtaat gccggcattt
     3061 ttcgccgtta ccatatccct gttgctgtcg cccacgtacc aggtatcggg gcgcgg
//
LOCUS       DASUEG010000287         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1640844, whole genome shotgun sequence.
ACCESSION   DASUEG010000287 DASUEG010000000
VERSION     DASUEG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640844"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFX11_05310"
     CDS             <1..516
                     /locus_tag="VFX11_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938069.1"
                     /translation="GLNAPPVGIDIVTDASQLVPWQGTEAEPFPDGDMPGNFALKIWA
                     DREDLQAIMDAYFRCNPIFGGSEQKCMSSHVYPGDMVGDVGGVYDAGTVTGGGTGGGT
                     VGGNWGGTIGGVIGSSGAVLEQAAPATGSIGTVTGTTAVTGATKDITAGTAVLNKASV
                     LDSEPLLLKQR"
     gene            849..2510
                     /locus_tag="VFX11_05315"
     CDS             849..2510
                     /locus_tag="VFX11_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938070.1"
                     /translation="MSLFTRAATFLLAILFLSACKSTETVLTVSYEGQGKITQGTKID
                     CGNTCQATYPVSTIEGVVYGMYNVKLLATPATGYDFFGWVDCGRDTMCNVTVEPDCAE
                     YNVGPNFVTCKRYATTFVSKKAIFVPKGSVVDSAWEGWSSCLVTTSDELKCWNHGVEA
                     AGLPSVDNPRAVEIGYKSGCVLDDSGLQCWGNAALNMPVINAPGDFVVEADFTCAIDG
                     GQVTCWPHERPADAIVNNVPELSNPVAIWVNPNARSLVCAEDDDGTHCWGGANVGQLT
                     PPALDNPSRIEAFGNTTCALTADGIRCWGESYDVAALNAQLNNATDISRPSSLGMCGV
                     DAGGARCIGKDHQTMTMAAEFQQAQKVDVSKSSMCTTQADGIVCGNLYYNPVTSFAPP
                     VKANGQLVAVGGPGGCAMTLADRRVSCWGGYDESWLSGNTNIAVSISNPKVLSVMPEN
                     RMWCAGGDGGLFCRRDAQGLDFYVSSFSVPTGLVGVTDIDMTDNVACAAHSNGVTCWG
                     VDSWGHQDVPALSHPAQVAVGTMHACALDDSGVVCWGLKPPRPQE"
     gene            complement(2585..4876)
                     /locus_tag="VFX11_05320"
     CDS             complement(2585..4876)
                     /locus_tag="VFX11_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005089949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX4938071.1"
                     /translation="MTAAEPVWKKTACILCSVNCGLEVQTDGRRITKIRGDDAHPASQ
                     GYVCEKSQRMDWYQNGADRLTSPMRRKADGSYEAIDWDTAIREIAQKFMAIKAKYGGE
                     SILYYGGGGQGNHLAGAYADCFLKAVGNQYRSNALAQEKTGEFWVQGKMLGTGVHGDF
                     EHCEVALFIGKNPWQSHGFARSRVLLNAIAKDPARSMIVIDPRRSETAALADFHLQIK
                     PGTDAWCLAALAGILVQENLIAQQWLAAHTTGYEQIAAELKKIPVAQFADICGVPEEK
                     LRAAAHRIASAKSVAAFEDLGMQMNLHSTLGSYLNRLIWLLTGNFGRIGTNNAFVPFL
                     SLSKASKGETSVSKKKAAPRVEKRSPVVNAKIIIGLIPCNVIPEEILTDHPKRYRAML
                     IESGNPLHSLADSQRMREALRALELSVVIDVAMTETARHADYVLPAASQFEKAEATFF
                     NLEFPRNAFHLRAPLFEPLPGTLPEAEIHARLLEALGELSEFDYLPLRLAAKLGRKAF
                     AAAFFAAVAVRPKTMKYAPVILYRTLGPTLPKGMESAAALWAICQLHVLGNRKTAAQA
                     GFTGVAPLAADRMFQQLLDNPSGVVFAVSTEEDSWNAVPMPGHRINLHIPELLPELAK
                     LSHERPPVDPAFPFILAAGERRSDTSNTAVRDTGWHKRGHYGTLRISPQDAEKIGCAD
                     GDLLRLSTRRGSVDVPAEISAMMQPGNISLPNGQGLDYRNAQGETVRKGVAPNELTDH
                     TQRDFLAGTPWHKYVPAKVERVV"
     gene            complement(4893..>5161)
                     /locus_tag="VFX11_05325"
     CDS             complement(4893..>5161)
                     /locus_tag="VFX11_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HEX4938072.1"
                     /translation="KPVFKLAGMTMLKAQGLGRMPRADVKAEFARHLDRIETALEPTG
                     WLVGTSKTIADIAVASQLLEIRRTTPHLLDPERRARLARWLDAI"
BASE COUNT          956 a         1505 c         1630 g         1070 t
ORIGIN      
        1 ggcctgaatg cgccgccggt gggcatcgat atcgtgaccg acgcatccca gctggtgccg
       61 tggcagggaa ccgaggccga gcctttcccc gatggcgaca tgccgggcaa tttcgcgctg
      121 aaaatctggg ccgaccgcga ggatctgcag gccatcatgg acgcctattt ccgctgcaac
      181 ccgatttttg gcggctcgga gcagaaatgc atgagcagtc atgtgtaccc gggcgatatg
      241 gtgggtgatg tgggcggtgt ttatgacgcg ggcactgtta ctggtggtgg cactggcgga
      301 ggcactgttg gcggcaactg gggcggtacc atcggcggcg tcatcggcag ttctggcgcg
      361 gtactggagc aggccgctcc ggccacgggc agtattggca ctgtcaccgg tacaacagct
      421 gtcaccggtg caacaaaaga catcacagca ggaacggctg tactgaacaa agccagcgtg
      481 ctggattcgg aacccttgct gttgaaacag cgctgatccg caacactgga aaagagcacg
      541 agcattgtca tggcagactc gcaccattga agcgtcgctc gcagcgtttc aatgaaggcc
      601 gtggcagcag ttgcaagggc aggccgctcc gattggggag cggccttttt ctatcgtctt
      661 cttgcacgaa ttgtcttctg gcgcgaattt tcattcgaca attattgatt gttctatcac
      721 tttcactgcc gtgtgatccc gttcttgcgt tgctcctgct cctgcgttaa caatagctgt
      781 gcgataggca atttttcgcg ctgtttgccc catcaaaata ataaagctat caactcaggg
      841 agagagttat gtcgctgttt acgcgcgcgg ccacgtttct gctggccatc ctgtttttat
      901 ccgcctgcaa atccacggaa accgtcctca ccgtcagtta tgaaggtcag ggcaaaatca
      961 cccagggcac caaaatcgat tgcggcaata cctgtcaggc cacctatccg gtatccacta
     1021 tcgaaggcgt ggtgtatggc atgtacaacg tgaaactgct ggccacgccc gctaccggtt
     1081 acgatttttt cggctgggta gactgcggcc gcgataccat gtgcaatgtc acggtggaac
     1141 cggattgtgc cgagtacaac gtcggcccca attttgtcac ctgtaaacgc tacgccacca
     1201 cttttgtttc caaaaaggcc atatttgtgc ccaaaggcag tgtggtggat tccgcctggg
     1261 aaggctggag cagttgcctg gtcactacat ccgacgaact caagtgctgg aatcatggtg
     1321 tggaggccgc cgggttgccc agcgtcgaca atccgcgcgc tgttgagatc ggatacaagt
     1381 ccggctgcgt gctcgacgac agcgggctgc aatgctgggg taacgctgca ctgaatatgc
     1441 cggtgatcaa tgcgcccggg gatttcgtgg tggaagctga tttcacctgt gccatcgacg
     1501 gtggtcaggt gacgtgctgg ccccacgagc ggcctgctga cgccatcgtg aacaacgtgc
     1561 cggaactgtc caacccggtg gcaatctggg tgaatccgaa tgcacgttcg ctggtgtgtg
     1621 cagaggatga cgatggcacc cattgctggg gcggggcgaa tgtgggccag ctgacgccgc
     1681 ctgcgctgga caatccttcc cgtatcgaag cgttcggcaa caccacctgc gcgctcactg
     1741 cggatggaat ccggtgctgg ggcgagtcct atgacgtcgc cgcactcaac gcgcagctga
     1801 ataacgcgac ggatatttcc aggccgtcca gcctgggcat gtgcggcgtt gatgccggtg
     1861 gtgcgcgctg cattggcaag gaccaccaga ccatgacgat ggccgcggaa ttccaacagg
     1921 cgcaaaaggt ggatgtcagc aagagcagca tgtgcaccac gcaagcggat ggtatcgttt
     1981 gcggtaattt gtattacaac ccggtgacca gttttgcgcc gcccgtgaaa gccaacggcc
     2041 agctggtggc ggtgggtggt cccggtggct gtgcgatgac actggcggac cggcgggttt
     2101 cctgctgggg cggctatgac gaaagctggt tgagcggcaa caccaatatc gccgtatcca
     2161 tcagcaaccc gaaagtgctg tcagtgatgc ctgagaatcg catgtggtgt gccggtggtg
     2221 atggcgggct gttctgccgg cgcgatgcgc aggggctgga tttttacgtg agcagtttca
     2281 gcgtgcccac ggggctggtg ggtgtcaccg acatcgacat gaccgacaat gtcgcctgcg
     2341 ccgcgcacag taacggcgtt acctgctggg gcgtggacag ctggggccat caggatgtac
     2401 cggcgctcag ccatcctgct caggtggcag taggtaccat gcatgcctgc gcgctggacg
     2461 acagcggcgt agtgtgctgg ggattgaaac cgccgcggcc gcaggaataa accgggccgg
     2521 aaaaaatggc gcggttccct gggagccgcg ccctgtctgt gaaagcattt tcattttctg
     2581 cagttcacac cacccgctcg accttggccg gaacatattt atgccagggt gtgcccgcca
     2641 gaaaatcccg ctgcgtgtgg tcggtcagtt cattgggtgc cacgccttta cgcacggttt
     2701 caccctgcgc attgcgatag tccaggccct ggccattggg cagcgaaata ttccccggct
     2761 gcatcatcgc ggaaatttct gccggcacgt ccacgctgcc gcgtcgcgtg ctcagccgca
     2821 gcagatcgcc atcggcgcaa ccgattttct ccgcatcctg cgggctgatg cgcagcgtgc
     2881 cgtagtgacc gcgcttgtgc cagccggtgt cacgcacggc ggtgttgctg gtgtcgctgc
     2941 gacgttctcc ggcagcgaga ataaagggaa acgctggatc gacaggcggc cgctcatggg
     3001 acagtttcgc cagttccggc agcagctcgg gaatatgcag gttgatgcga tggcccggca
     3061 tgggcactgc attccagctg tcttcttccg tggaaaccgc gaacaccacg ccagaggggt
     3121 tatccagcag ttgctggaac atccggtccg ccgccagcgg cgcaacgccg gtgaagcccg
     3181 cctgtgccgc ggttttgcga ttgcccagca catgcagctg gcaaatggcc cagagtgcgg
     3241 cggcggattc cattcctttg ggcagcgtcg gccccagggt gcggtacaga atgaccggcg
     3301 catacttcat ggttttgggc cgcaccgcca ccgcggcaaa aaatgctgcc gcgaatgcct
     3361 tgcgccccag cttcgctgcc agccgcaacg gcagataatc gaattcgctc agttcaccca
     3421 gggcttccag caggcgggcg tggatctccg cttcgggcag cgtgccgggg agtggctcga
     3481 acaggggcgc gcgcaggtga aacgcattgc gcggaaactc cagattgaaa aatgtcgcct
     3541 cggctttttc aaactggctg gctgcgggca gcacataatc cgcgtgacgc gcggtttccg
     3601 tcatcgccac atcgatcacc accgacagct ccagtgcgcg cagggcttca cgcatgcgct
     3661 ggctgtccgc cagcgagtgc agcggattgc cgctttcgat cagcatggcg cgataccgtt
     3721 tcgggtgatc ggtaagaatt tcttcgggaa tcacattgca gggaatcagg ccgatgatga
     3781 ttttcgcgtt gaccaccgga ctgcgttttt ccacccgtgg cgccgccttc ttcttgctga
     3841 cgctggtttc acccttgctg gctttcgaca gtgacagaaa cggcacgaat gcgttattgg
     3901 tgccaatgcg gccaaagttg ccggtaagca gccagatcag gcggttcaga taactgccga
     3961 gggtggaatg cagattcatc tgcatgccca ggtcttcaaa tgcggcgaca cttttggcgg
     4021 acgcaatgcg gtgggcggcg gcacgcaatt tttcttccgg cacgccgcaa atgtcagcaa
     4081 attgtgcgac gggaattttt ttcagttcag cagcgatctg ctcatagccg gtggtgtgtg
     4141 ccgccagcca ttgctgcgcg atcaggtttt cctgcaccag aatgcctgcc agcgcggcca
     4201 gacaccaggc gtcggtgccg ggtttgatct gcaggtgaaa gtccgccagc gccgccgtct
     4261 cgctgcggcg cggatcgatc acgatcatgg agcgggccgg atctttcgcg atggcgttga
     4321 gcaatacgcg ggagcgcgcg aagccgtggg attgccacgg attcttgccg atgaacaggg
     4381 ccacttcgca atgctcgaaa tcgccgtgca cgccggtgcc gagcatcttg ccctgcaccc
     4441 agaattcgcc ggtcttttcc tgcgccagcg cattcgagcg gtactggttg ccgaccgcct
     4501 tgaggaagca atcggcgtag gcgccggcca ggtgattgcc ctgcccgccg ccaccgtagt
     4561 acaggatcga ctcgccgccg tatttcgcct tgatcgccat gaacttctgc gcgatctcgc
     4621 ggatcgccgt gtcccagtcg atggcctcgt agctgccatc ggctttgcga cgcatcggcg
     4681 aggtcaggcg gtcggcaccg ttctggtacc agtccatgcg ctgcgatttc tcgcacacgt
     4741 agccctgcga cgccgggtgc gcgtcgtcgc cgcggatctt ggtgatgcgc cggccgtcgg
     4801 tctggacctc gaggccgcag ttgacgctgc acaggatgca ggcggttttc ttccagacag
     4861 gttcggcagc agtcatgggc ggctccggag aatcagatcg catcgagcca gcgcgccagg
     4921 cgcgcccggc gttcggggtc gagaagatgc ggcgtggtgc gacggatttc gagcagctgc
     4981 gaggcgacgg cgatgtcggc aatcgtcttg ctcgtgccga ccagccagcc ggtcggttcg
     5041 agcgccgtct cgatgcgatc gaggtgacgc gcgaactcgg ccttgacgtc cgcacgcggc
     5101 atgcggccca gtccctgcgc cttgagcatc gtcatgccgg cgagcttgaa caccggcttg
     5161 a
//
LOCUS       DASUEG010000288         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1290219, whole genome shotgun sequence.
ACCESSION   DASUEG010000288 DASUEG010000000
VERSION     DASUEG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290219"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFX11_05330"
     CDS             <1..694
                     /locus_tag="VFX11_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX4938073.1"
                     /translation="IAGFAFGNSDTTLEYSEAQNQTDNDTDHYIVYASWYRDNFAVDY
                     IVGYAKGEFDTQRKILDSNAVGNTDNELSYLNIAGSYDFVSGGLTYGPFASYDYLDGT
                     IDNFEETNGGGWEAAFDDQDVKSQIYAVGGHASYAMSYGWGVLVPHARAEWRNELEDE
                     RDLIVGRFVQDPVSSFAITPDEPDGDWYQVSAGVSAQFAHGIAAFLDYEEVLEYEDTD
                     LSIVTLGARWEM"
     gene            976..1296
                     /locus_tag="VFX11_05335"
     CDS             976..1296
                     /locus_tag="VFX11_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938074.1"
                     /translation="MCSADDEPLREFQYFNEDGIFIGKSEGTAPDEQLFNQAHYVFDD
                     HDDVVKNLDLLAIMRRRLLNLRRELVNVPVQQMERIMEINRQIRVLEQNIVEIQNQGW
                     KRAG"
     gene            complement(1421..2551)
                     /locus_tag="VFX11_05340"
     CDS             complement(1421..2551)
                     /locus_tag="VFX11_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4938075.1"
                     /translation="MNFDLSEEQQMLVDSIGKFLKNDYPFETRRKLANSELGFSAQNW
                     KTFAELGWLSVPFSADNGGFGGTTVDMMLMHEQFGTALVTEPLIPTLILGGRLIELLG
                     DNAQKETLLPAVIQGELQLALAYNERIARNNPAVVGCTARRDGDSYVLNGEKIAVLNG
                     HAANQILVTARLSGDVSSTNGISVFLVDANAAGLQRKSYQTVDGLRAADLQLNNVKVP
                     AANLLGTEGAAFDAIETVLDEATLALCAEAVGAMEVLYKTTVDYSKTRKQFGMPIGKF
                     QVLQFRMVDMFMAHEQSKSMIYMAALRSLEGRAAARKAVSAVKVQIGKAGRKIGQEAV
                     QLHGGMGMTDELNVGYYFKRLTAIDALFGNVDFHLARYARVA"
BASE COUNT          594 a          841 c          798 g          600 t
ORIGIN      
        1 gattgccggt tttgccttcg gcaacagcga caccaccctg gaatacagcg aagcccagaa
       61 ccagaccgac aacgacaccg atcactacat tgtttatgcc agctggtacc gcgacaattt
      121 tgccgtggac tacatcgtgg gctacgccaa gggcgaattc gacacccagc gcaaaattct
      181 ggacagcaac gctgtcggca ataccgacaa cgaactcagt tacctcaaca ttgccggttc
      241 ctatgatttt gtcagcggtg gcctgaccta tggcccgttt gccagctacg actatctgga
      301 cggcaccatc gacaacttcg aggaaaccaa cggcggtggc tgggaagcag ccttcgacga
      361 ccaggatgtg aaatcccaga tctatgccgt gggcggtcac gcctcctacg ccatgagtta
      421 cggctggggt gtactggtac cccacgcccg cgccgagtgg cgcaacgagc tggaagacga
      481 gcgcgacctg atcgtcggcc gctttgtgca ggaccccgtc agcagcttcg ccatcacccc
      541 cgacgagccg gatggcgact ggtaccaggt gagcgccggt gtatcggcac agtttgccca
      601 tggcatcgcc gcgtttctgg attacgagga agtgctggaa tacgaagaca ccgacctgag
      661 catcgtcacg ctgggtgcac gctgggaaat gtgatcttcc cgcacgtcat cgctttccag
      721 acaaaccagc agccccgccc acgccagtgg ccggggcttt ttgttggggg ccatggctgg
      781 tacaggattg ggcgggaggc acattacttc cccgcctgct gtttagtcca aattcgacta
      841 acaacgccaa ccgactgaat taattgcgaa ttcacacgaa caacccgtgc gctcggcggt
      901 cacgcccttg cgcgatgccg ctttcgcatc taacaagaat aaggttgtct tttgtttttt
      961 tgtggaacca ctttcatgtg cagcgcggat gacgaaccgc ttcgggagtt tcaatacttc
     1021 aacgaagacg ggatttttat cgggaaaagt gagggcacgg cgccggatga acagctgttc
     1081 aatcaggccc attacgtgtt cgacgatcac gacgacgtag tgaaaaacct ggacctgctg
     1141 gcgatcatgc gacgacggct gctgaacctg cggcgcgaac tggtgaatgt gccggtgcag
     1201 cagatggaga ggatcatgga gatcaaccgc cagatccggg tgctggagca gaacatcgtc
     1261 gagatccaga accagggctg gaaacgggcc gggtaatttt gataaatgga taattgcgcg
     1321 caaaaaaagg agccctttct acaccaccac tccgggtgat tcgaaaaggg ctcgtcaagg
     1381 ttgagcaaca gtctgaaatg ggttgaaatg gtgacgcgga tcaggccacg cgagcgtaac
     1441 gtgccaggtg gaaatccaca ttgccgaaca gcgcgtcaat ggcggtgaga cgtttgaagt
     1501 agtagcccac gttcaattcg tcggtcatcc ccatgccgcc gtgcaactgc accgcttcct
     1561 ggccaatctt gcgtcctgcc ttgccgatct gcactttcac cgccgacacc gccttgcgcg
     1621 ccgcagcgcg gccttccagc gaccgcagtg ccgccatgta gatcatggat ttgctctgct
     1681 catgcgccat gaacatatcc accatgcgga actgcagcac ctggaatttg ccgatgggca
     1741 tgccgaactg tttgcgggtc ttgctgtagt cgacggtggt cttgtacagc acttccatcg
     1801 caccgacagc ctcggcacag agggcgagag tggcttcgtc cagcacggtt tcaatggcgt
     1861 cgaacgcggc gccttcggtg cccagcaggt tcgccgcagg cacttttacg ttattcagtt
     1921 gcagatccgc cgcgcgcagg ccgtcaacag tctgataaga cttgcgctgc aggccggcag
     1981 cattcgcatc caccaggaac acgctgatgc cgtttgtgct gctgacatct ccgctcaggc
     2041 gcgcggtcac cagaatctgg ttggcggcgt ggccgttcag cacggcaatt ttctcgccgt
     2101 tcagaacata gctgtcgcca tcacgtcgcg cagtgcaacc gacgacagcc ggattgttgc
     2161 gagcgatgcg ctcgttgtag gccagcgcca gttgcagttc gccctggatc acggcgggca
     2221 gcagggtttc cttctgcgcg ttgtcgccca gcagttcgat caggcgaccg cccaggatca
     2281 gggtggggat cagcggctcg gtcaccaacg cagtgccgaa ttgttcgtgc atcagcatca
     2341 tgtcgacggt ggtgccaccg aagccgccgt tgtccgctga aaatggcaca ctcagccagc
     2401 ccagctcggc gaatgttttc cagttctgtg cactgaaacc cagctccgaa ttggccagct
     2461 tgcggcgggt ttcaaacgga taatcgtttt tcaggaattt gccgatgctg tctaccagca
     2521 tctgttgttc ttcgctcaga tcgaaattca tttcgtgcct ctcaattgct ctgtgaggtg
     2581 tgcgtgtggc actagtcgaa tctggacaca ccgtgaaccc atccctgggg gctgctctgc
     2641 ggcccttcgg ggccggccac tcacctacag gttcgtggcg ttcgcagggg aaaagcaatg
     2701 cttttcctta tcctgctcac ccatgccgca aagcgtccag cttcgactag tgcctcacgc
     2761 acacttcgca cattccttac aaacccagca ccattttcgc gatgatgccg cgctgcacta
     2821 cgttggaacc gcc
//
LOCUS       DASUEG010000289         6497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_946933, whole genome shotgun sequence.
ACCESSION   DASUEG010000289 DASUEG010000000
VERSION     DASUEG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946933"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFX11_05345"
     CDS             complement(<1..990)
                     /locus_tag="VFX11_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4938076.1"
                     /translation="MIPLQPTELTLAEALTLAIRDLGVNHVFGVSGANIESFHDALFR
                     LARHDITSLLCKSERGASFIADGFARQSGALAVCCSTSGGGMMNLAVGLAEARNSGIP
                     VLAIIGCPPTALAGRGAFQDGSGGRDRIDAELLWQAVAKFSATVTPAKFWLQLQQAIN
                     ATREGFKGPAVLLIPSDSFNAPVAARPAFFGATACSGSGAIPAHQLYQLELALRRSRH
                     PLLIAGPALSGNRELQRFSFTTGIPVATTMESTAAFPHHHPRFMGVLGVAGHRNVHDY
                     LRTCDLVVSLGSQLDIMTRGAALEALDSIPIHIYHTSTASAPVSNLIRLCECDP"
     gene            complement(996..1994)
                     /locus_tag="VFX11_05350"
     CDS             complement(996..1994)
                     /locus_tag="VFX11_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HEX4938077.1"
                     /translation="MEPISLQGVASYLPDIVVDNDFFRLASGIDRHPMFRGTHLRHHI
                     DQDESATSMAVKAIRRLADKLDLDVARDVDVLITNVTLADLPFVGCGAAIAGELNLNP
                     RYIYDLQNGGCVSFIFMLELAQLILQSTPARSALLCNLQTAAGRIFSLEDNRKLPQSC
                     VPGDGCGVAYVTREGNNPVIATVTRTHGNYANDMDVKAPDGRHWWQPGTTAFNIDFDE
                     SKIASIVARGNRLVPEALNSALKLAGLGSKDVDFLVTNQPNRIFLRNWRESVQLREEQ
                     HIQTLADHGNLFGAASPICLERGIDDGRIHPASNLLLGGFSHAGDYSAAAIVRWNG"
     gene            complement(2015..2560)
                     /locus_tag="VFX11_05355"
     CDS             complement(2015..2560)
                     /locus_tag="VFX11_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4938078.1"
                     /translation="MQERCMDMTHSVYPHDAIFGNYCTIQEYIHCPPQTTFDYLAETS
                     NLLEWTYSLRDMTYDEERQLYEFTDAVGGKTKCFCRTVAHRDAMTVDYHCAWDQGDEL
                     WMIYLMRVIPAETVLKKPGSVVLWTNCHHPYYEKNPYPQQAPADRKIWVGDMWPMFYA
                     GHWIEMQNLKKILEHRHAGLR"
     gene            3011..5065
                     /gene="ligA"
                     /locus_tag="VFX11_05360"
     CDS             3011..5065
                     /gene="ligA"
                     /locus_tag="VFX11_05360"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4938079.1"
                     /translation="MPSAAPTAIAQQLENLREQLREHNYRYYVLDTPSVPDAEYDRLF
                     RQLQQLEQEHPDLVTPDSPTQRVGDRPLDAFSQVTHRVPMLSLDNVFNDQELVAFDRR
                     VRDRLKTEDEIEYVCEPKLDGLAVSVLYENGVLVQAATRGDGQTGEDITQNIRTIQSL
                     PLKLHGAGYPSTLEVRGEVFMPRAGFEEMNRRAAENGEKVFVNPRNAAAGSLRQLDAR
                     ITAQRPLDMYCYGVGFTAGGELAQTHYDNLQLLKSWGLRINAETTLAHGAAELQQYHD
                     AMLAKRNQLAYEIDGIVYKVNSLALQKELGFVSRAPRWATAHKFPAQEEMTELLGVDF
                     QVGRTGALTPVARLAPVFVGGVTVSNATLHNMDEVARMDVRVGDTVIVHRAGDVIPKV
                     VKVVLDKRPEHTQPILMPAHCPVCGSDIVKPEGEAVARCSGGLYCPAQVKEAIKHFAS
                     RKAMDVEGLGDKLVEQLVDQGLIAHVSDLYGLRVEPVAAMERMGQKSAQNLIDALEAS
                     KATTLPRFLFALGIRDVGEATALTLAQHFGTLENIRNASEEQLQEAPDVGPVVASRIA
                     EFFRQPHNLEIIRALREAGVHWPEQEPAAREAGALDGKTYVLTGTLTKLTRDEAKDYL
                     QQLGAKVAGSVSKNTDVVVAGEKAGSKLKKAEELGIAIIDEDAFIQLLRDHDIKLGNE
                     GF"
     gene            5068..5700
                     /locus_tag="VFX11_05365"
     CDS             5068..5700
                     /locus_tag="VFX11_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938080.1"
                     /translation="MSETGHHTHIPFRENRFLIFSLVLMLLAFLLTGWIAWLNYSMHR
                     SATTQILKSQQLQERAQAHVQDQYTEYLKQEAANRRFEKAFEIKQRHYADFMGAVSDA
                     WLAVNRSDTTLLENALHQMRKSCYGLEPFLDTGGRHFLQKRIALFEKLSTQLASGAEY
                     RYKVNQDADKAKLNGMSDEFQAFLYPLLFEPEAAQETQSGDRDETTQTGQ"
     gene            5675..>6497
                     /locus_tag="VFX11_05370"
     CDS             5675..>6497
                     /locus_tag="VFX11_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEX4938081.1"
                     /translation="MKPRKLVNEKFRLKDMPTRPDKGIDHPSEQIDELQLDLLRAQLA
                     LFKENKRAIILIEGTDTAGKGGIIRRITRHLDPRSAHVWPIGAPERWEQSRHYLFRFW
                     QRLPEPGELTIFDRSWYGRVLVERVEGHANAEEWKRAYREINEFERQLTEDGVILIKL
                     FLHLSKDEQYARFMERIREPTKRWKIKVSDLESRRFWNEYQRAYDDMLNRTATDFAPW
                     FVLSADDKDYARVHALKLIGKQLKKHVDLQRVNLLDPQVIAAIIDQFGEDVLRDDL"
BASE COUNT         1344 a         1857 c         2016 g         1280 t
ORIGIN      
        1 tggatcgcat tcgcacagcc ggatcaggtt tgacacgggc gcgctggcgg tggacgtgtg
       61 gtagatatgg atgggaatgg aatccagcgc ttccagtgcg gcgccccgcg tcatgatgtc
      121 cagttgcgat cccaggctca caaccagatc gcaggtgcgc aggtagtcgt gcacgttgcg
      181 atgaccggcc acgcccagca cgcccatgaa acgcggatga tgatggggaa aagccgcggt
      241 cgattccatg gtggtggcca cgggaatgcc ggtggtgaaa ctgaatcgct gcagttcgcg
      301 gttgccgctc agggccgggc ctgcaatcag cagcgggtga cgggatcggc gcagtgccag
      361 ttccagctgg tatagctggt gagccggaat tgcgccgctg ccggagcagg ccgtcgcgcc
      421 aaagaatgcg ggccgtgccg ccacgggagc attgaagctg tcgctgggaa tcagtaacac
      481 ggccgggccc ttgaagcctt cgcgggtcgc gttgatcgcc tgttgcagct gcaaccagaa
      541 tttggcggga gtgacggtcg cgctgaactt ggcgacagcc tgccacagca gttcggcgtc
      601 gatccggtcg cgtccaccgg agccatcctg gaacgcaccc cgccccgcca gagcggtggg
      661 cgggcaaccg atgatggcga gtacgggaat gccggaattg cgcgcctctg ccagaccgac
      721 ggcgagattc atcatgccgc caccggaggt ggaacagcat actgccagcg cgccactctg
      781 gcgggcgaag ccatctgcta tgaagctggc gccacgctcg ctcttgcaga gcaggctggt
      841 gatgtcgtga cgggccaggc ggaacaacgc atcatggaac gattcaatgt tggcgccgct
      901 gacgccgaat acatggttca cgcccagatc gcgaatcgcc agcgtcagcg cttccgccag
      961 tgtcagttct gtcggctgca gggggatcat caacgctatc cgttccagcg tacgatggcg
     1021 gcggcgctgt aatcccccgc gtgggaaaat ccacccagca gcaggttgct ggccgggtgg
     1081 atgcggccat cgtcgatgcc ccgttccagg cagatgggac tcgcggcgcc gaacaggttg
     1141 ccgtgatcag ccaaagtctg gatatgctgc tcttcccgca gttgtacgga ttcgcgccag
     1201 ttccgcagga aaatccggtt gggctggttg gttaccagaa aatccacgtc cttgctgcca
     1261 agcccggcca gtttcagcgc actgttcagt gcctctggta ccaggcgatt gcctcgggca
     1321 acgatcgacg caatcttcga ttcatcaaaa tcgatgttga aggcggtagt gccaggttgc
     1381 caccagtgcc gtccgtccgg cgctttcacg tccatgtcgt tggcatagtt gccgtgagtg
     1441 cgggtgacgg tggcgatcac gggattgttg ccttcgcggg tgacataggc gacgccgcaa
     1501 ccatcgcccg gcacgcagga ttgcggcagt ttgcgattgt cctccagcga gaatatccgg
     1561 ccggctgcgg tctgcagatt gcacagtagg gcggaacgtg ccggtgtgga ttgcagaatc
     1621 agttgcgcca gttccagcat gaaaataaag gaaacacagc caccattctg cagatcgtaa
     1681 atataacgcg ggttcaggtt cagctcgccg gcaatcgcag caccgcagcc gacgaaaggc
     1741 aggtctgcca gggtcacgtt ggtaatgagt acatccacat cgcgcgccac gtccagatcc
     1801 agcttgtccg ccagtcgacg aatggctttc acggccatgc tggtggcgga ttcgtcctga
     1861 tcaatatggt ggcgcagatg ggtgccgcga aacatgggat gacgatcaat gccggatgcc
     1921 agccggaaaa aatcattgtc cacgacgatg tcgggcagat agctcgctac gccttgcagg
     1981 ctaatgggtt ccatcctctg tctcctgtgc ctgttcagcg caagccggcg tgccggtgct
     2041 caagaatttt tttcagattc tgcatttcga tccagtgacc ggcgtagaac atgggccaca
     2101 tgtcacccac ccagattttg cggtcggcag gggcttgctg cggatagggg tttttctcgt
     2161 agtaggggtg atggcagttg gtccagagca caacagatcc cggctttttc agcaccgttt
     2221 cggccggaat gacccgcatc agataaatca tccagagttc atcgccctgg tcccaggcgc
     2281 agtgatagtc cacggtcatg gcgtctctgt gcgcaacggt gcggcagaag catttggtct
     2341 tgccgccaac agcgtcagtg aattcgtaca gctgccgttc ctcgtcatag gtcatgtcgc
     2401 gcaggctgta ggtccattcc agcaggttgg aagtttctgc caggtaatca aatgtggttt
     2461 gcggagggca gtgaatatat tcctggatgg tgcagtagtt gccgaaaatg gcgtcgtgcg
     2521 gataaacaga atgcgtcata tccatgcagc gctcctgcat gtcttctttc tgcacgtttt
     2581 cgatgcgaca aagatcggga attgtgtcga gcagaatggg ggtgctcatg atgtgaactc
     2641 ctttttttgt tgtgcgcttc tgtccgcgat gcgcagttta gaccgttcga tcccgattca
     2701 atcagggttt attttgttat ttttcgtata tgtttttttg ttgagcaaag cgagctgacc
     2761 gtccgcgatt acagcgtcgc attccgaaaa tgcagtggcg aattacccta ccttcggagg
     2821 gggcgtgatt catttttccg ggcaacctgc cgccggcaat tggcgcgtct tcaggtgcga
     2881 atgaggcgcc cgggtcgacc tgtcctgcag ccaggtcctc aacccctgtc cgtcgaaggg
     2941 gcgggctgtg tataattcgc gtacacctga gttcctataa cgttcgctcc cataacgacc
     3001 ggattccgca atgccttctg ctgcaccgac tgccatcgcc caacaactgg aaaacctgcg
     3061 ggaacagctc cgcgagcata actaccgcta ttacgtcctc gacaccccca gcgtccctga
     3121 tgcggaatac gaccgcctgt tccgccagtt gcagcagctg gagcaggaac acccggatct
     3181 ggtcaccccc gattctccga cccagcgtgt gggcgaccgg ccgctggatg cgttttccca
     3241 ggtgacgcac cgcgtgccca tgctgtcgct ggacaacgtg ttcaacgatc aggaactggt
     3301 ggcatttgat cgccgtgtgc gtgaccgcct gaaaacggaa gacgaaatcg aatacgtgtg
     3361 cgagcccaag ctggatggcc tggccgtgag cgtgctgtat gaaaacggtg tgctggtgca
     3421 ggcggccacc cggggtgacg gccagactgg cgaggacatt acccagaaca tccgcaccat
     3481 ccagtcgttg ccgctgaaac tgcatggcgc cggctatcct tccaccctgg aagtgcgggg
     3541 agaggtgttc atgccccggg cgggattcga agaaatgaac cggcgggcgg cggaaaatgg
     3601 cgagaaggtg tttgtaaatc cccgcaacgc ggccgctggc agtctgcgcc agctggatgc
     3661 gcgcattacc gcgcagcgcc cgctggacat gtactgctac ggtgtgggtt tcactgcggg
     3721 tggtgagctg gcgcagaccc actacgacaa tctgcaactg ctgaagtcct ggggtctgcg
     3781 tatcaatgcc gaaacgacac tggcacacgg tgcggcggag ctgcagcagt accatgacgc
     3841 catgctggca aagcggaatc agctggccta tgaaatcgac ggcattgtct acaaggtgaa
     3901 ttccctggcg ctgcagaaag aactgggttt cgtgtcgcgg gcgccacgct gggccacggc
     3961 gcacaagttc ccggcgcagg aggaaatgac cgaattgctg ggggtggact tccaggtggg
     4021 ccgcaccggt gcgctgacgc cggtggcacg gctggcaccg gtgttcgttg gcggtgtcac
     4081 ggtcagtaat gccacgctgc acaacatgga tgaagtggca cgcatggatg tgcgggtggg
     4141 cgacacggtc atcgtgcacc gcgccggcga cgtgattccg aaagtggtga aggtggtact
     4201 ggataagcgt ccagaacaca cccagccgat actgatgccc gctcactgcc cggtgtgcgg
     4261 ttccgacatc gtcaagcccg agggcgaggc ggtggcgcgc tgctccggcg gcctgtattg
     4321 tccggcgcag gtgaaggaag ccatcaagca ttttgcatcg cgcaaggcga tggatgtgga
     4381 aggtctgggt gacaagctgg tggagcagct ggtggaccag ggtctgatcg cccacgtgtc
     4441 ggacctgtac gggctgcggg tggaacctgt cgcggcgatg gagcgcatgg gccagaaatc
     4501 ggcccagaac ctgatcgacg cgctggaagc cagcaaagcc accaccttgc cccggttcct
     4561 cttcgcgctg ggcattcgcg atgtaggcga ggccaccgcg ctcaccctgg cccagcattt
     4621 cggcacgctg gaaaatatcc gcaatgccag cgaagagcag ttgcaggaag cccccgatgt
     4681 ggggcctgtt gtggccagcc gcatcgccga atttttccgc cagccccata acctggaaat
     4741 catccgcgcc ctgcgagaag ccggtgtaca ctggccagag caggaacctg ccgcgcggga
     4801 agcgggtgcg ctcgatggca aaacctacgt cctcaccgga acgctgacca aactcacccg
     4861 cgacgaagcc aaggactatc tgcagcaact gggtgcaaaa gtggcgggca gcgtgtcgaa
     4921 aaacacggat gtggtggtgg cgggtgaaaa agccggttcc aagctgaaaa aggccgagga
     4981 gctcggcatc gccatcatcg acgaggacgc attcatccag ttactgcgcg atcacgacat
     5041 caagcttggc aacgagggat tctgatcatg tccgaaacgg gccaccacac gcacataccg
     5101 ttcagggaaa accgcttcct gattttcagt ctggtgctga tgctgctggc atttctgctc
     5161 actggctgga ttgcttggct gaattactcc atgcaccgtt ctgccaccac gcagatcctg
     5221 aaatcgcagc agctgcagga gcgggcccag gcccacgtgc aggatcagta cacggagtac
     5281 ctgaagcagg aagccgccaa tcgccgcttt gaaaaggcct tcgaaatcaa acagcggcat
     5341 tatgcggatt tcatgggtgc ggtttccgat gcctggctgg cggtgaaccg cagcgacacc
     5401 acgctgctgg aaaatgccct gcaccagatg cgcaaatcct gctacggcct ggagccgttc
     5461 ttggacaccg gcggccgcca ctttctgcaa aaacgcatcg cgctgtttga aaagctgtcg
     5521 acgcagctgg ccagcggtgc cgagtaccgt tataaagtga accaggatgc ggacaaggcc
     5581 aagctcaacg gcatgagtga tgaattccag gcctttcttt atcccttgtt attcgaaccg
     5641 gaagcagcgc aggaaactca atcaggagat cgggatgaaa ccacgcaaac tggtcaatga
     5701 aaagttcaga ctcaaggaca tgccgacccg ccccgacaag ggtatcgatc atcccagcga
     5761 acagattgac gaactgcaac tggacctgct gcgggcgcag ctggcgctgt tcaaggaaaa
     5821 caaacgcgcc atcatcctga tcgagggcac tgacactgcc ggcaagggcg gcatcatccg
     5881 ccgcatcacc cgccatctcg atcctcgcag cgcccacgtc tggcccatcg gcgcgccgga
     5941 gcgctgggag cagtcgcgtc attacctgtt ccgcttctgg cagcgtctgc cggaacccgg
     6001 tgagctgacc attttcgacc gttcctggta cggccgcgtg ctggtggagc gggtggaagg
     6061 ccatgccaac gcggaggagt ggaaacgcgc ctatcgcgaa atcaatgaat tcgagcgcca
     6121 gcttaccgag gatggcgtga ttctcatcaa actgttcctg catttgtcga aggacgagca
     6181 gtatgcgcgt ttcatggagc gtatccgcga accgaccaaa cgctggaaga tcaaggtgtc
     6241 ggatctggag agccggcgtt tctggaatga ataccagcgc gcctatgacg acatgctcaa
     6301 ccgcactgcc acggatttcg cgccctggtt cgtgctcagc gctgacgaca aggattatgc
     6361 ccgcgtgcat gccctgaagc tgatcggcaa gcagctgaaa aagcacgtgg atctgcagcg
     6421 ggtgaatctg ctggacccgc aggtgatcgc ggccattatc gatcaattcg gcgaagacgt
     6481 tctgcgggac gatctgg
//
LOCUS       DASUEG010000290         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_932000, whole genome shotgun sequence.
ACCESSION   DASUEG010000290 DASUEG010000000
VERSION     DASUEG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932000"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFX11_05375"
     CDS             <1..293
                     /locus_tag="VFX11_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4938082.1"
                     /translation="ATAADGYVDITLRNDSGYALIEVEDNGSGMDADFVKNRLFRPFD
                     TTKSGKGMGIGVYQTREFIRNLGCDVTVKSEPGIGTTFTIYIPVEAQAKAVS"
     gene            324..1688
                     /gene="prsR"
                     /locus_tag="VFX11_05380"
     CDS             324..1688
                     /gene="prsR"
                     /locus_tag="VFX11_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX4938083.1"
                     /translation="MSEHKPALLIVEDDLGLQSQLRWHFDKYEVITASDRKSAIAAVR
                     MHEPAVVLQDLGLPPDEEGVEEGFATIQETLRLAPSTKIIVVTGYHDYENALRAVALG
                     AYDFYQKPVNTDTLDLIVQRAHQMWSLEQENKRLQETRAAPLPGVIASDERMHSVCRT
                     VEKLAPTDVTCLILGESGTGKEVIARGIHNLSKRRDRRFVAINCAAVPENLMESELFG
                     YEKGAFTGAAKRTLGKVEVAHEGTLFLDEIGDMPLPLQAKLLRFLQERVIERLGGREE
                     IRVDVRVVCATNKNLSEMVAEGTFREDLYYRISEIVVELPPLRERGGDKILIARHLLT
                     KYVDQLSKGIRGFTPEAAAAIESYEWPGNIREMENKIKRAVIMCDQRNVGIADLGLPQ
                     PDDISLNLRTVRQDAERAAIIRAISMSDSNISAAAKLLGITRPTLYDLIKKYNIHVSE
                     NCKD"
     gene            1692..>3820
                     /locus_tag="VFX11_05385"
     CDS             1692..>3820
                     /locus_tag="VFX11_05385"
                     /inference="COORDINATES: protein motif:HMM:NF020855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4938084.1"
                     /translation="MLRMSAHLLVLLLALVFAPGCEKASQPENKSAEILKRAESYFAQ
                     GQFNAATIEVKNALKENNSNLDAFLLLARIYLEQGNYNNAAKVLLELPQDNLQVVILL
                     GQTYQHQGKYKSLFQLLQPLKNRDDARDSVEFQLLLARSLARHEAPQEAQTLLQTLAG
                     RLTRPEQLAELEVVRAQAWQAQGDPTRQMEALDSALRHQPNNIDALVEKARLYIQNRN
                     YEAAEDVLSQALMALPSTDTITLKRLTILQAMVATLTRQGRSVEAMVYSKLIAEANPK
                     AQELQAEFQSAVDQLKAGNLKEAEAILSKLYSSEHAALAGSVLGLIKFQQGEFEQAAQ
                     LLQETVDPETASPEALRAFAESQLRLQNPEQALKTIEANVSEHPNDPDILGIYGLSLL
                     ATQHDEKGIETIKAALKLDPSRSRLRMALVDTYNRQGKYDEALQQLEAAYSTTPDDIV
                     VQERLVKQYRVMKKDSALALLVDALAKKTAPQSQALAGLALLASDPARAAVLLDKAYQ
                     AAPVDLSVLRAQVARYTVAGDHHNSLRFAKELIALNVDDLFALSVATQAEIAQKRDAA
                     AVEYLKSLSEQNANAWGPDFVLARYNLAAGKIDQALQHAESALARSAFNRTTTALTTQ
                     LHFLKAQQLAANRQFAEARETLMKGLQIDPSNLELMHLLVAVELEEKNIKSAEKLVGE
                     MEQMASGSHFAYMAAGDLAQAKGEQSAA"
BASE COUNT          907 a         1152 c         1086 g          675 t
ORIGIN      
        1 aagccaccgc agccgatgga tacgtggaca taacactgcg caacgacagt ggctatgccc
       61 tcatcgaagt ggaagacaac ggttcgggca tggacgcgga ctttgtcaaa aaccgcctgt
      121 tccgtccttt cgacaccacc aaatccggca aaggcatggg catcggcgtg taccagacgc
      181 gggaattcat ccgcaacctc ggttgcgatg tcaccgtaaa aagcgaaccc ggcatcggca
      241 ccacctttac gatttacatt ccggtcgaag cccaggccaa agccgtatcc taacaacaag
      301 cctatacgat ggaccgcctc cccatgtcag aacacaagcc cgcacttctc atcgttgaag
      361 atgatcttgg gctgcaaagc cagctgcgtt ggcatttcga taaatacgag gtgatcaccg
      421 cttcggaccg caagagcgcc attgctgccg tacgcatgca cgaacccgcg gtggtgttgc
      481 aggatctggg tctgccgccg gacgaggaag gtgtcgagga aggcttcgcc accattcagg
      541 aaaccctgcg cctggcaccc tccaccaaga tcatcgttgt cactggttac cacgattatg
      601 aaaatgcgct gcgggcggtg gcactcggcg cttatgattt ctaccagaaa cccgtcaata
      661 ccgacacact ggatctgatc gtgcagcgcg cccatcagat gtggtcgctg gaacaggaaa
      721 acaagcggct gcaggaaacc cgcgcagcgc ccctgcccgg cgtgatcgcc tcggacgagc
      781 gcatgcacag tgtctgccgc acggtggaga agctggcgcc taccgacgtg acctgtctga
      841 tcctcggtga atccggcacc ggcaaggaag tcattgcacg cggcattcac aatctgagca
      901 aacgccgcga tcgccgtttt gtggccatca attgtgctgc agtgccggaa aatctgatgg
      961 aaagcgagct gttcggctac gagaaaggcg cctttaccgg cgcggccaag cgcacactgg
     1021 gcaaggtgga agtggcgcac gaaggcaccc tgtttctgga cgagatcggc gacatgccgc
     1081 tgccgctgca ggccaaatta ctgcgttttc tgcaggaacg cgtcatcgag cgactgggtg
     1141 gacgcgaaga aatccgggtc gatgtgcgtg tggtctgtgc caccaacaag aatctaagtg
     1201 aaatggtggc ggaaggaact ttccgcgagg atctttacta ccgcatcagt gaaatcgtgg
     1261 tggaattgcc gcccttgcgg gaacggggtg gtgacaagat cctgattgcg cgacatctgc
     1321 tgaccaagta tgtggatcag ctcagcaaag gcattcgcgg cttcacgccc gaagccgccg
     1381 ccgccatcga gtcctacgaa tggccgggca acatccgcga aatggaaaac aagatcaagc
     1441 gtgctgtgat catgtgcgat cagcgcaacg tgggaattgc agacctggga ctgcctcaac
     1501 cggacgacat ttcactgaac ctgcgtaccg tacgccagga tgccgagcgc gctgccatca
     1561 tccgcgccat cagcatgtcc gacagcaata tttccgcggc ggcaaaactg ctgggcatca
     1621 cccgcccgac gctctacgat ctgatcaaga aatacaatat tcacgtatct gaaaactgca
     1681 aggactgaat catgctgcgt atgtctgccc acctgctcgt actgctgctg gcgctggtat
     1741 tcgcccccgg gtgcgaaaaa gccagccagc ccgagaacaa atcagcagaa atcctgaagc
     1801 gggctgaaag ctactttgcg cagggccagt tcaatgccgc caccatcgag gtaaagaacg
     1861 cgctgaagga aaacaacagc aacctggatg cctttctgct gctggcacga atctatctgg
     1921 aacagggcaa ctacaacaat gccgccaagg tactgctgga actgccgcag gacaatctgc
     1981 aggttgtcat cctcctgggc caaacctatc agcatcaggg taaatacaaa tccctcttcc
     2041 agctgttgca gcctttgaaa aaccgcgatg acgcgcgcga ctccgtcgaa ttccagctcc
     2101 tgctggcccg cagtctggcc cgtcacgagg cgccacagga agcacagacc ctgttgcaga
     2161 cactcgctgg ccggctgacc aggcccgaac agctggcaga actggaagtg gtgcgcgcgc
     2221 aggcctggca ggcccagggc gatccgaccc ggcagatgga agcgctggac agcgccctgc
     2281 gccaccagcc caacaatatc gacgcgctgg tggagaaggc ccgcctttac atccagaacc
     2341 gcaattacga ggcagctgaa gatgtgctga gccaggccct gatggcgttg ccttcaacgg
     2401 ataccatcac cctcaagcgg ctcaccatcc tgcaggccat ggtggcgacg ctgacccgtc
     2461 agggccgttc tgtggaagcc atggtctatt ccaagctgat tgccgaggcc aatcccaagg
     2521 cccaggaact gcaggcggaa ttccagtccg cggtggacca gctcaaggcc ggcaatctca
     2581 aggaagccga agccattctc agcaaactct acagcagcga acacgcggca ctggccggat
     2641 cggtgctggg cctgatcaag ttccagcagg gcgaattcga gcaggcggct cagctgctgc
     2701 aggaaactgt cgatcccgag acggcttccc cggaagcgct gcgcgcattt gccgaatcgc
     2761 aactgcggct acaaaatccg gagcaggcac tcaaaaccat tgaggccaac gtcagcgaac
     2821 atcccaatga tccggatatc ctcggcatct acggattgtc actgcttgcc actcagcatg
     2881 acgagaaagg gatcgaaacc atcaaggccg ccttgaaact cgatccgtca cgatcgcgtc
     2941 tgcggatggc cctggtggac acctataacc ggcagggcaa atacgacgag gccctgcagc
     3001 agctggaagc cgcttacagc accacgccgg acgacattgt ggtgcaggaa cggctggtca
     3061 aacagtaccg cgtcatgaaa aaggattcgg cgctggcgct actggtggat gccctggcaa
     3121 agaaaacagc gccccaaagc caggcgctgg cggggcttgc cttgcttgcc agcgatcctg
     3181 cgcgagccgc ggtactgctg gacaaagcct accaggcagc gccagtcgat ctgtcggtcc
     3241 tgcgagccca ggtcgcacgc tacacagtgg ccggcgacca tcacaacagc ctgcgcttcg
     3301 caaaggagct gatcgccctc aatgtggatg atctgttcgc cttgtcggta gccacccagg
     3361 cggagatcgc gcagaagcgg gacgcagcag cggtggagta tctgaaatcc ctgagcgaac
     3421 agaatgccaa tgcgtggggt cctgattttg tattggcccg ctacaacctg gcggcaggta
     3481 aaatcgacca agcccttcag catgccgaaa gcgcccttgc ccgctccgcc ttcaatcgca
     3541 caaccacagc actgacaacc cagttgcatt tcctgaaggc gcaacagctg gccgccaacc
     3601 gacagtttgc cgaggctcgt gaaaccctga tgaaaggctt gcagattgat ccctccaatc
     3661 tcgagctgat gcatctgctg gtggcggtcg agctggaaga aaaaaatatc aagtcggcgg
     3721 aaaaactcgt gggcgaaatg gagcagatgg cttccggctc ccattttgcc tacatggccg
     3781 ccggcgatct tgcccaggcc aagggtgaac agagcgcagc
//
LOCUS       DASUEG010000291         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_22008, whole genome shotgun sequence.
ACCESSION   DASUEG010000291 DASUEG010000000
VERSION     DASUEG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22008"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VFX11_05390"
     CDS             <1..1819
                     /locus_tag="VFX11_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4938085.1"
                     /translation="QGKPQWLDGFLMDITRRKEMEQELFQAKERAEQAAAARSAFLAN
                     MSHEIRTPMNAIIGFSDVLMGTALNPEQHRHLSIISSAARSLLHLLNDILDSAKLEKG
                     KLVLESLDFSLPELLDAVLSTLGMQARRKGLALNLNLHPQLADHFVGAPDRIRQVLTN
                     IVGNAIKFTEQGKVDLDVRPERPGFLLFRVTDTGIGIAPDRLESIFEPFTQADASMSR
                     RFGGTGLGTTISKQLVELMGGWIRVHSTPGKGSCFEFCLPLPPGRRAEAGAGTSSVRL
                     PALQVLAVDDIQQNLDLLSLLLGKAGHQVSLAHDGLEALEKVQQQRFDLILMDMQMPR
                     LDGLAAARRIRDWQRQQGERPSAIIALTASVLDEDKLAARDAGMDGFSSKPVDLDSLN
                     REIARVLGIALPTPAPRAAARAVNSVLNWEQGLKRWDDAALYIRELTTFAIHYGRLAL
                     DLDSLQHQQDYASLAQRAHGARGVAANLSIDGLVEPLAQLERAAHSREMDACADAVDR
                     LAQALPRFGAELATLLRQLQPPEAVITPSPDPRAFATALDRLRRAAAASEWDDLALAD
                     LIRLRPVHLSGPVQRISDAFNNFDFDLALTALDQLQSEL"
     gene            1851..2855
                     /locus_tag="VFX11_05395"
     CDS             1851..2855
                     /locus_tag="VFX11_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4938086.1"
                     /translation="MTQSLLPRQEQRPKILVADDEPTNLKVLKQILQDDYRLVFAKSG
                     DDAIALATRELPGLILLDVMMPGMTGFEACQRLKSAPVTARIPVIFVTALKEAVDETT
                     GFAVGGVDYISKPVVPAIVRARVKTHLSLVQADELKRTRLQIIQRLGRAAEYKDNETG
                     LHVMRMSHYSRVLALAWGFSADMAEDLLNAAPMHDIGKIGIPDSIMLKPGKLTDEEFT
                     MMKRHPLIGAEILGESDSQLLELARSVALYHHEKWDGSGYPHGLKGEDIPVEARIVAL
                     ADVFDALTSKRPYKDAWPVEQALEHIRQQSGKHFDPQLVNLLQQQLPAILEIKERWAE
                     "
     gene            complement(2876..>3241)
                     /locus_tag="VFX11_05400"
     CDS             complement(2876..>3241)
                     /locus_tag="VFX11_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEX4938087.1"
                     /translation="RGRTVVERGLDRVQGGFVRQQFIRFLAIFGAVSASFFIFYNIPA
                     QWVGMHSDPWPEDHMKRSYFVGGICGEGTDKPCPHPSLPIPTKHSGYINTEGRLVLPE
                     GVEMPPVVPLERADREDNQ"
BASE COUNT          608 a         1006 c         1054 g          573 t
ORIGIN      
        1 acagggcaaa ccgcagtggc tggacggttt cctgatggac atcacccggc gcaaggaaat
       61 ggagcaggaa ctgttccagg cgaaggaacg ggcggagcag gcggccgccg cacgcagcgc
      121 gtttctcgcc aacatgagcc acgagatccg cacgcccatg aacgccatca tcggcttcag
      181 cgacgtactg atgggcaccg cgctgaatcc cgagcagcat cgccacctgt ccatcatcag
      241 cagtgcggcg cgctcattgc tgcatctgct gaacgacatt ctcgacagcg ccaagctgga
      301 gaagggcaag ctggtgctgg aatccctgga tttctcgctg ccggaactgc tggacgcggt
      361 actgtccacg ctcggcatgc aggcgcggcg caaggggctg gcgctgaacc tgaatctgca
      421 tccgcagctg gcggatcatt tcgtgggcgc gccagaccgc atccgccagg tgctcaccaa
      481 catcgtcggc aacgcgatca agttcaccga gcagggcaag gtggatctgg atgtgcggcc
      541 cgagcggccg ggttttctgc tgttccgcgt taccgacacc ggcattggca ttgcaccgga
      601 ccggctggaa tcgattttcg aacccttcac ccaggccgat gcctccatga gccggcgctt
      661 cggcggcacg ggtctgggca ccaccatcag caagcagctg gtggaactga tgggtggctg
      721 gattcgtgtc cacagcaccc cgggcaaggg cagctgtttc gagttctgcc tgccgttgcc
      781 acccggccgc agggccgaag cgggcgccgg caccagcagc gtgcggctgc cggcactgca
      841 ggtgctggcg gtggatgaca tccagcagaa cctggatctg ctgagcttgc tgctgggcaa
      901 ggcggggcac caggtcagcc tggcgcacga tggcctggag gcgctggaaa aggtgcagca
      961 gcaacgcttc gatttgatcc tgatggacat gcagatgccg cgtctggatg gcctggcggc
     1021 ggcacgccgc atccgcgact ggcagcggca gcagggcgag cgccccagtg cgatcatcgc
     1081 cctgaccgcc agtgtgctgg acgaggacaa gctggcagcc cgcgacgccg gcatggacgg
     1141 tttttccagc aaaccagtgg atctggacag tttgaaccgc gaaattgccc gggtgctggg
     1201 cattgccctg ccgactccgg ccccccgcgc cgccgcccgt gccgttaaca gcgtgctgaa
     1261 ctgggagcag ggcctgaaac gctgggacga tgccgcgctg tatatccgcg agctgaccac
     1321 cttcgccatc cactatggcc gtctggcgct ggatctggat tcgctgcagc accagcagga
     1381 ttacgcgtcc ctggcccagc gcgctcatgg tgccaggggg gtggccgcca atctgtccat
     1441 cgacgggctg gtggagccgc tggcacaact ggaacgggcc gcccacagcc gtgaaatgga
     1501 tgcctgcgcc gatgccgtgg accggctggc ccaggccctg ccgcggtttg gtgccgagct
     1561 ggccacgctg ttgcggcaat tgcagccgcc ggaagcggtc atcaccccaa gtccggaccc
     1621 gcgggcattt gcgacggcgc tggatcggct gcgccgggcc gctgccgcca gcgagtggga
     1681 cgatctggcc ctggcggacc tgatccggct gcggcccgtg cacctgtccg gtccggtgca
     1741 gcgcatttcc gacgccttca acaattttga tttcgatctg gcgctgacgg cgctggatca
     1801 attgcagtct gaactctgat gcaaccgctc acagcaacgg tgatatgacc atgacccagt
     1861 ccctgctgcc ccgacaggaa cagcgcccca aaatcctggt ggcggacgac gagcccacca
     1921 acctgaaagt gctgaagcag atcctgcagg acgactaccg cctggtattt gcgaaaagtg
     1981 gcgacgacgc catcgccctg gccacgcggg aactgccggg gctgattctg ctggatgtga
     2041 tgatgccggg catgaccggt ttcgaagcct gccagcggct gaaatccgct cctgtcaccg
     2101 cgcgtattcc ggtgatcttc gtcaccgcgc tgaaagaggc ggtggatgaa accaccggtt
     2161 ttgccgtggg cggcgtggat tacatcagca agcccgtggt gcccgccatt gtgcgggcgc
     2221 gggtgaaaac ccatctgtcg ctggtgcagg cggacgaact caagcgcacc cgtctgcaga
     2281 tcatccagcg cctgggccgt gctgctgaat acaaggacaa cgaaaccggc ctgcacgtga
     2341 tgcgcatgag ccactattcc cgcgtgctgg cgctggcctg gggcttttcc gccgacatgg
     2401 ccgaggatct gctcaacgcc gcgcccatgc acgacatcgg caagatcggc attcccgaca
     2461 gcatcatgct gaaaccgggc aagctcaccg acgaggaatt cacgatgatg aagcggcatc
     2521 cgctgatcgg tgccgagatt ctgggagaaa gcgattcaca gctgctggaa ctggcgcgct
     2581 cggtggcgct gtaccaccac gaaaaatggg atggcagcgg ttatcctcac gggttgaaag
     2641 gcgaggacat tccggtggag gcgcgcatcg tggcactggc agatgtgttc gacgccctca
     2701 ccagcaagcg cccctacaag gacgcctggc cggtggagca ggcgctggag cacatccgcc
     2761 agcagagcgg caagcatttc gatccgcagc tggtgaatct gttgcagcag caattgccgg
     2821 ccattctgga aattaaagag cgctgggcgg agtagggcgt gatttgaaat ctcgcttatt
     2881 gattgtcctc gcgatccgct cgctcgagag gcacgacggg aggcatctcc acgccttccg
     2941 gcaggacgag tcttccttcg gtattgatat aacccgaatg cttggtcggt atcggcaggc
     3001 tgggatgggg gcagggtttg tcggttcctt caccgcagat gcctccgacg aaataggagc
     3061 gtttcatgtg gtcctccggc catgggtcgg agtgcatgcc gacccactgc gccggtatgt
     3121 tgtagaagat gaagaagctc gcgctgactg cgccgaagat tgccaggaat cgtatgaact
     3181 gctgtctgac gaacccgccc tggaccctgt cgagcccgcg ttcaacgacg gtgcgacccc
     3241 g
//
LOCUS       DASUEG010000292         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1442078, whole genome shotgun sequence.
ACCESSION   DASUEG010000292 DASUEG010000000
VERSION     DASUEG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442078"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFX11_05405"
     CDS             <1..409
                     /locus_tag="VFX11_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4938088.1"
                     /translation="YPNFYIMLGPYALIGTSYFKMVEGNAIHISRCIREALRRKATRV
                     EIRQTVHDRYFQNIQKRQQNTVFLNHNCALSNSYYFDHHGDAPMLRPSTSVEMLWRAK
                     HLSMDNYLFLKSNGAKRRFRPETSNQYEDHSML"
     gene            complement(363..1595)
                     /locus_tag="VFX11_05410"
     CDS             complement(363..1595)
                     /locus_tag="VFX11_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4938089.1"
                     /translation="MTISEKQTTVIVGGGHAAGALMTILLQKKYQHEVVLVGEEPHPP
                     YQRPPLSKNYLAGEVDQASLYLKPRVVYEKAGQQLRLGVRVEQIDRDNKTIRLSDQST
                     LKYDQLVLATGSHVRRLRAPGSDLQGIHYLHDITDTDALREKLVPGKRLVIVGGGYIG
                     LEVAASATKKGVNVTVLEAADRLMQRVTGPEMSAFFYAKHRGAGVDVRLNTAVTGFEA
                     GDQGRVAGVTLADGGTVPADVVLVSIGVIPETALAEAAGLPCDDGIVVDEFARTGDPD
                     ILAIGDCTRHRNLFFGKMQRLESVANAVDQARTAAATLMGEEKPYDSAPWFWSNQYDV
                     RLQMVGLSQNSDQRVLRGSAEDKEFAVFYLREGSVIAVDAVNLPVAFMVGKQLVQHRK
                     SVCAKALSDPRTDLKSLV"
     gene            complement(1800..3215)
                     /locus_tag="VFX11_05415"
     CDS             complement(1800..3215)
                     /locus_tag="VFX11_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4938090.1"
                     /translation="MSTKSSATDVIQAKMINATSRVVPMHLQIQALKMLVKAKKKIIG
                     ARRPPLNFVEASVPDVNTLALEDIDLSNPFLYRQDQWRAYFKRLRDEAPVHYQKNSLF
                     GPFWSVTRYEDILFVDKNHELFSSEPQIVLGDPPEGLSVEMFIAMDPPNHDVQRRAVQ
                     GVVAPQNLKEMEGLIRTRAGDVLDSLPMGEAFNWVPTVSKELTGRMLATLLDFPYEER
                     HKLVDWSDRLSGAASATGGEFTDEDGMFDDAADMAWSFSRLWRDKEARRAAGEEPGFD
                     LISMLQSSADTKDLINRPMEFIGNLALLIVGGNDTTRNSMSGGVLALNQFPEEFAKLK
                     ANPELIPNMVSEIIRWQTPLAHMRRVATQDVEMHGNTIRKGDRVVMWYASGNRDERKF
                     ENPDQFIIDRKDARNHMSFGYGVHRCMGNRLAELQLRILWEELLKRFDDIEVVGEPVR
                     VQSNFVRGYSELMVKLTPKQK"
     gene            complement(3230..3550)
                     /locus_tag="VFX11_05420"
     CDS             complement(3230..3550)
                     /locus_tag="VFX11_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4938091.1"
                     /translation="MGKITFIEHDNTEHVTEFEAGTTLMQIAVDNAVPGIDGDCGGEC
                     ACGTCHMIVADEWFGKTGSIGDAEEQMLSMTPERAQTSRLGCQVRTTEAMDGMTVHLP
                     EFQM"
     gene            3669..>4148
                     /locus_tag="VFX11_05425"
     CDS             3669..>4148
                     /locus_tag="VFX11_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4938092.1"
                     /translation="MGKPMAQQADGWQNLEASIPSNYSRIIARELDLTVRQLPILLRG
                     TALDVEQFLSEDSLLTPAQQVRVLRNALALSGQPEFGLRLGKRLTPATHGAMGFAASS
                     SPDLLSALRAIHTFLPTRASVIQLHLQQVEEHLECRLDFQVPLNADLQRCVADTIVKV
                     "
BASE COUNT          906 a         1273 c         1060 g          909 t
ORIGIN      
        1 gtatccgaac ttttacatca tgctcgggcc ctatgcattg attggcacct cctacttcaa
       61 gatggtggag ggaaatgcca ttcatatttc acgctgcatc cgcgaggccc tccggcgcaa
      121 ggcaacccgg gtggaaatcc ggcagaccgt gcatgaccgc tactttcaga acattcagaa
      181 acggcagcag aacaccgtat ttctgaacca caactgcgca ctctccaaca gctattactt
      241 cgaccaccat ggcgacgcgc ccatgctgcg cccctcgacc tcggtggaaa tgctgtggcg
      301 agcaaagcat ctgtcgatgg acaattacct gtttctgaag agcaacggcg cgaaacgccg
      361 tttcagacca gagacttcaa atcagtacga ggatcactca atgctttagc gcaaacgctc
      421 ttgcggtgct gcaccaactg cttgccaacc ataaaggcaa ccggcagatt gacagcatca
      481 acagcgatga cactgccctc gcggagatag aacaccgcaa attccttatc ctcagcgctg
      541 ccgcgcagca cgcgctgatc actattttgc gatagcccca ccatctgcag gcgaacgtca
      601 tactgattcg accagaacca cggggcgcta tcgtaaggct tctcctcgcc catcagagtg
      661 gccgctgcag tacgggcctg atcgaccgcg ttggcgacag actcaagccg ttgcattttc
      721 ccgaagaaaa gattgcggtg gcgggtacag tcgccaatcg ccaggatgtc aggatcaccg
      781 gtgcgggcaa actcatccac gacaatacca tcatcacagg gcagtccggc ggcctcagcc
      841 agcgcggttt ccggtataac gccaatggaa acgagcacaa catcagccgg cacagtgccg
      901 ccatccgcca acgtcacacc tgccacacga ccttgatcgc ccgcttcgaa accggttacc
      961 gccgtgttca gacgtacgtc cacaccggcg ccacggtgtt tggcgtaaaa gaacgcggac
     1021 atctccgggc ctgttacgcg ctgcataaga cgatcagcgg cttccagcac cgtgacattc
     1081 acgcctttct tggtagcgct ggctgccacc tcaaggccga tataaccgcc accgacgatg
     1141 accaaacgct tccccggaac caatttctca cgtagagcat ctgtgtcagt tatgtcgtgt
     1201 aggtaatgaa tgccctgcaa gtcagacccg ggtgcccgca aacgccggac gtgtgaaccc
     1261 gtagccagta ccagttgatc gtatttcaac gtgctctgat ccgacaacct gatagtcttg
     1321 ttgtcccgat caatttgttc gacgcgcacg ccgagccgca actgctgtcc tgctttttca
     1381 tacaccacgc gcggcttcag gtacagcgac gcctgatcaa cctctcctgc cagataattc
     1441 ttggacaggg gaggtcgctg gtagggtgga tggggctctt cgcctaccag aaccacctca
     1501 tgctgatatt ttttctgcag caagattgtc atgagcgcac ccgctgcgtg cccgccacca
     1561 acgatgacag tggtctgctt ctcacttatt gtcatgctcc ccctctccac tgcttctgta
     1621 actgaactcg cctcggcact accgggttcc ataaatcagc actacgccat tgatgtcgag
     1681 cgagttcgcg ccagccattt atcaaaccgg gatcgtagtc aaagaccata acactctgcg
     1741 gacacctggc tttccctgtt cccgcagagt gtttaatcag gtcttctctt ccaccagaac
     1801 tacttctgct ttggcgtcag tttaaccatc agctcagagt agccgcgcac gaagttcgac
     1861 tgcacccgaa ctggctcgcc gactacttcg atgtcgtcaa agcgcttgag cagttcttcc
     1921 cagagaatgc gcagctgcaa ttcagccaga cggtttccca tacaacggtg aacgccatag
     1981 ccaaacgaca tatgatttcg cgcgtcctta cgatcgataa tgaattgatc ggggttttcg
     2041 aattttctct cgtcccgatt gcccgaggcg taccacatca ccacacgatc acctttcctg
     2101 atggtatttc catgcatctc cacgtcctgc gtggcgacac ggcgcatatg cgccagcggg
     2161 gtttgccagc ggatgatttc cgagaccatg ttcggaatca gctccgggtt cgccttcagt
     2221 ttggcgaatt cctcggggaa ctgattcaag gccagcacgc cgccgctcat ggagttgcgg
     2281 gtggtgtcat tgccaccaac gataagcagc gccaggttcc cgataaactc catcggacga
     2341 ttgatcaaat cctttgtatc tgcgctgctc tgcagcatgc tgatcaaatc gaaaccaggt
     2401 tcttcgccag ccgcgcggcg tgcctccttg tcgcgccaaa gtctggagaa cgaccacgcc
     2461 atatctgcgg cgtcgtcaaa catgccatct tcatcggtaa actcaccgcc ggttgccgat
     2521 gctgcgccgg acaatctatc cgaccagtca accagcttgt gacgttcctc ataagggaag
     2581 tcaagcaacg tggccagcat gcgcccggtc agttccttcg acacggtcgg cacccaattg
     2641 aaggcttcgc ccataggcag actgtcgagc acgtccccgg cacgcgtgcg gatcagccct
     2701 tccatttcct tcaaattctg cggcgcgacc acaccctgaa ccgcccggcg ctgcacatca
     2761 tgattgggcg gatccatggc gataaacatt tccaccgaca gcccttccgg gggatcgccc
     2821 agcacgattt gcggctcgga ggaaaacagc tcgtggttct tgtccacgaa cagaatgtct
     2881 tcatagcgtg tcaccgacca gaatggtccg aataaactgt tcttttggta gtgcaccggt
     2941 gcctcatcac ggagccgctt gaagtaggca cgccactgat cttgccggta taaaaaagga
     3001 ttgctgagat cgatgtcctc aagcgccagt gtgttgacat cgggcacaga cgcttcgacg
     3061 aagttgagcg ggggccgccg cgcgccgatg atcttcttct ttgccttcac caacattttc
     3121 agcgcctgga tttgcaagtg cattggcacc actctggagg tggcgttgat cattttggcc
     3181 tgaattacat cagtcgcgct tgatttcgtt gacatggcgt cctccgtgct tacatctgga
     3241 attcgggcaa atgcacggtc atgccgtcca tcgcctcggt ggttcgaacc tgacagccga
     3301 gacgcgaggt ttgcgcccgc tccggggtca tggacagcat ctgctcctca gcgtcgccga
     3361 tcgatccggt cttgccgaac cactcatccg caacgatcat gtggcaggtg ccgcaggcgc
     3421 actccccgcc acaatcaccg tcgataccgg gaacggcatt atcgacggca atctgcatca
     3481 gcgtggttcc agcctcaaat tctgtgacat gttcggtgtt gtcatgctca ataaaggtaa
     3541 tcttgcccac tgcctctctc ctgcgagtaa cttactgcat tggatagtga ttcgaagcga
     3601 acacggcgac gaggtcattt cttgacactg tgggggtcat ttagagacaa tgtcaccgca
     3661 taggagcaat gggcaagccg atggcacaac aggcagatgg ttggcaaaat cttgaagcta
     3721 gtattccgtc gaactattcg cggattatcg cccgtgagct ggacctgaca gtcagacagt
     3781 tgcccatttt actgcgtggc acggctcttg acgtcgagca gttcctgagc gaggacagcc
     3841 tgctcacacc ggcccagcag gtacgggtct tgcgcaacgc actggcgctg tccggccagc
     3901 cggaattcgg attacgtctg ggcaaacgcc tgaccccggc aacccacggt gcgatgggat
     3961 ttgccgcctc cagcagcccg gacctgctca gcgcattacg ggcgatacac accttcctgc
     4021 ccacccgcgc cagcgttatc cagctgcatt tgcagcaggt tgaagaacat ctggagtgca
     4081 ggctggattt tcaggtgccg ctgaacgcag acctccagcg ctgcgtggcg gacacgattg
     4141 tcaaggtg
//
LOCUS       DASUEG010000293         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2040473, whole genome shotgun sequence.
ACCESSION   DASUEG010000293 DASUEG010000000
VERSION     DASUEG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040473"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1837)
                     /locus_tag="VFX11_05430"
     CDS             complement(50..1837)
                     /locus_tag="VFX11_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4938093.1"
                     /translation="MQSHDNYDKEVDVLVVGSGAGALTAAVVARQEGCAEVLVVEKSD
                     RYGGTSAMSGGGIWIPNSHYARQQGVKDSASEAFTYLKHVIGDEVSEERLRTYVEKAP
                     EMLEYMANHSRLQYETVPYSDYYPEKPGGKEGHRTHQPTPMNAKHLGEHFKTLREPPP
                     QVLVAGKLTMTMKEGRAFLTQAKGWMWTAVMMALKYYLDIPGRLQGPRSRRLTMGNAL
                     MGQLRWSMIERGIDLWLKSPLLELIVENGAVTGAVIERDGKKMRIRARKGVIIGAGGF
                     EHNQQMREQYLPQPTSAEWSGSQINNTGDGIQAAMKIGADTALMNHAWWAPSVKIPAW
                     DRPYVIFAERSLPGLVIVNKAGKRFQNEAAPYLESGLAFYQADKPEEPTVPSFVVFDA
                     VFRKKFPFGPIPPGYSSPDSRISRKVWEVLVKADTIEELAKKIGVDAKGLAETVRKNN
                     EYARSGKDADFKRGESYYDRYYGDSKTSPNPCIAPIAQGPFYAIPIHPGDIGTKGGVL
                     TDVNGQALDKSGAPIPGLYVIGNSAASVMGSKYPGAGATLGPAMTFGYLAAKHIASGA
                     AHAGVSRNSAGTTKKAGSASAPSAAAARV"
     gene            2085..>3326
                     /locus_tag="VFX11_05435"
     CDS             2085..>3326
                     /locus_tag="VFX11_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938094.1"
                     /translation="MITPALTESYLRGPASQPLLTDTIGQCFDRIAATFPEREALVVR
                     HQNIRWTFAQYQHQVNALATGLLALGVQRGDRVGIWSPNRVEWCLTQFATAKIGAIMV
                     CINPAYRLYELEYALNKVECKVVVTAEKFKTSEYLKMLNELAPEIARSTPGQLDSHRL
                     PHLKTVIRMGAEKSPGMLNFADVCTLGGAAEQRQLAALATQLQPDDAINIQFTSGTTG
                     NPKGATLTHANILNNGLQVGKGMHLSEQDRLCIPVPLYHCFGMVMGNLAALTHGACAV
                     FPNDAFDPVSVLETVAAERCTALHGVPTMFIAELDCPDFDKYDLSSLRTGIMAGAPCP
                     IDVMKRVIGKMHMRDVTIMYGQTETSPVNHMTAIDAPIEKRVSTVGKVGPHQEVKIVD
                     AAGRTVPIGEKGEICCRGYSVM"
BASE COUNT          665 a         1033 c          947 g          681 t
ORIGIN      
        1 tttattcttt ttgacggatc gtcatttatt gcggttattg cggtgcaggt taaacacggg
       61 cggccgctgc cgacggcgcg ctggccgagc cggctttttt cgtcgtccct gcagaattcc
      121 ggctgacgcc ggcgtgggcc gcgccactgg caatgtgttt ggcagccaga taaccgaagg
      181 tcatcgcagg acccagtgtg gcgccggcac cggggtattt gctgcccatt accgatgcgg
      241 cggagttgcc aatcacgtac aaaccgggaa tcggcgcgcc agatttatcc agtgcctgac
      301 cattcacatc cgtgagtacg ccgcccttgg tgccgatgtc gccggggtga atcggaatgg
      361 cgtagaacgg cccctgcgcg atcggcgcaa tacagggatt cggcgaggtt ttgctgtcgc
      421 cgtagtaacg atcgtaatag ctttcaccgc gtttgaaatc cgcgtccttg ccacttctgg
      481 cgtattcgtt gttcttgcgc acggtttccg ccagaccttt tgcatcgaca ccgattttct
      541 tcgccagctc ttcgatggta tccgccttca ccagcacttc ccacaccttg cgcgagatgc
      601 ggctgtcggg cgagctgtaa ccgggcggga tcggaccaaa cgggaatttc ttgcggaaca
      661 cggcgtcgaa caccacgaag gatggcacgg tgggttcttc cggcttgtcg gcctggtaaa
      721 acgccagacc cgattccaga taaggtgctg cttcattctg gaagcgcttg cccgccttgt
      781 tcacgattac caggcccggc agcgagcgct cggcgaaaat tacgtaaggg cgatcccagg
      841 ccggaatttt cacggacggc gcccaccagg cgtgattcat cagcgcggtg tcggcgccga
      901 ttttcatggc cgcctgaatg ccgtcgccag tgttgttgat ctgggaaccg ctccactcgg
      961 cgctggtggg ttgcggcaga tactgttcgc gcatctgctg gttgtgctcg aaaccaccgg
     1021 cgccgatgat gacgcccttg cgcgcgcgaa tgcgcatttt tttgccgtcg cgctcgatca
     1081 ctgcgccagt cactgcgccg ttttccacga tcagctcgag cagcggcgat ttcagccaca
     1141 gatcaattcc acgttcgatc atggaccagc gcagttgacc catcagcgcg ttgcccatgg
     1201 taaggcggcg cgaacgcggg ccctgcaggc ggccgggaat atccaggtaa tatttcagcg
     1261 ccatcatcac cgccgtccac atccagccct tggcctgggt gaggaatgcg cggccttctt
     1321 tcatggtcat ggtgagcttg ccggccacca gtacctgcgg cggtggctca cgcagcgttt
     1381 tgaaatgctc gcccagatgt tttgcattca tcggcgtggg ctgatgcgtg cggtgacctt
     1441 ccttgccacc gggtttttcc gggtaatagt cggaataggg cacggtttca tattgcaggc
     1501 gggaatggtt cgccatgtac tccagcattt ccggcgcttt ttccacgtag gtgcgcaggc
     1561 gctcttccga cacttcgtcg ccgatcacat gcttcagata ggtgaacgcc tcggacgcgg
     1621 aatccttcac gccctgctgg cgcgcatagt gactgttggg aatccagatg ccgccaccgg
     1681 acatggcgga cgtgccgccg tagcggtcac ttttttccac caccagcacc tcggcgcagc
     1741 cttcctgccg ggccactacg gcggccgtca gcgctcccgc gcccgacccc accaccagca
     1801 catccacttc tttgtcataa ttatcatggc tttgcattgt tgcggattcc ccgaaaaagc
     1861 ccagcgaatg ccccattatc atgagttcac ccctattatg caatatattt gcaaaataga
     1921 tgtttatttg aatggggaat gcttctagaa tcgggcgaag cgcattaaaa ccggcaattt
     1981 gtggggcggc aggcccgctg ccactccgcc ggtaaaaacg gaaccctgcg caataaaaga
     2041 cagaataaca atcagaacaa acaggaatca gaaaggacgc caccatgatc acaccggcac
     2101 tcaccgaaag ctacctgcgc ggccccgcca gccagcccct gctgactgac accatcggcc
     2161 agtgtttcga ccgcatcgct gcaacctttc cggaacggga agccctggtg gtgcgtcacc
     2221 agaacatccg ctggacgttc gcccaatacc agcaccaggt gaatgcactg gccaccggac
     2281 tgctggcgct gggcgtgcag cggggcgacc gcgtcggcat ctggtcgccc aaccgggtgg
     2341 aatggtgtct tacccagttt gccaccgcca agattggtgc catcatggtg tgcatcaatc
     2401 ccgcgtaccg cctgtatgag ctggaatacg cgctcaacaa ggtggaatgc aaggtcgtcg
     2461 tcaccgcaga aaaattcaag accagcgaat acctgaaaat gctgaacgaa ctggcgccgg
     2521 aaattgcgcg cagcacaccg ggccaacttg attcccaccg cttgccgcat ctgaaaaccg
     2581 ttattcgcat gggcgcggaa aaatcgccgg gcatgctgaa ctttgccgac gtgtgcacgc
     2641 tgggcggcgc tgcggaacag cgacagctgg cggcactggc cacacagctg cagcccgacg
     2701 acgccatcaa tatccagttc accagcggca ccaccggcaa tccgaaaggc gccaccctca
     2761 cccacgccaa catcctcaac aacggcctgc aggtgggcaa gggcatgcat ctgagcgagc
     2821 aggatcgcct gtgcattccg gtgcctttgt accattgttt cggcatggta atgggtaatc
     2881 tggcggcgct cacccacggt gcctgcgccg ttttccccaa cgatgcattc gatcccgtca
     2941 gcgtgctgga aacggtcgcc gccgagagat gcacggcgct gcacggcgta cccaccatgt
     3001 tcatcgccga actggattgt ccggatttcg acaaatatga cctgtccagc ctgcgcaccg
     3061 gcatcatggc cggcgccccc tgccccatcg acgtgatgaa gcgagtgatc ggcaagatgc
     3121 acatgcgcga cgtcaccatc atgtacgggc agacggaaac cagccccgtg aaccacatga
     3181 ccgccatcga cgcgccgatt gaaaaacgcg tgtccaccgt cggcaaggtg gggcctcacc
     3241 aggaagtgaa aatcgtcgac gccgccggcc gcaccgtgcc gattggcgag aagggtgaaa
     3301 tctgctgccg tggctattcc gtcatg
//
LOCUS       DASUEG010000294         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_864053, whole genome shotgun sequence.
ACCESSION   DASUEG010000294 DASUEG010000000
VERSION     DASUEG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864053"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1434)
                     /locus_tag="VFX11_05440"
     CDS             complement(214..1434)
                     /locus_tag="VFX11_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX4938095.1"
                     /translation="MITLKAAPRGRDIHEEHRASTPLELLFDLTFVVAIALAAAQLHH
                     GVVEHHVLQTLPGYLLAFLAIWWAWMNYTWFASAYDNDDTLYRVLTMMQMGGVLVLAT
                     GIPGLFTGQYTASVVGYVVMRLALCAQWLRAAHGDPLRRKTCLRYAGGIVLVQLGWVG
                     FLLAVVNGVLTGATPWAAIAVLWVFELAVPMWAEKAGTTPWHAHHIAERYGLMVIIVL
                     GECVLGATNTVATMWQAQGWSFDLALLGFAGTLLIFSLWWMYFLLPSADALHHHRERA
                     WGWGYGHYILFAAVAAVGAGLEVVADVLKASHAAVDVHAVSPLYAISTVAVAEAIFVG
                     SLWALNCYATRARNQQLGLTLVCLASIALGPIAVAQGLSLPLGLLVLSLGPLIAIAYN
                     EHGRRHCADHFKVQ"
     gene            complement(1490..1891)
                     /locus_tag="VFX11_05445"
     CDS             complement(1490..1891)
                     /locus_tag="VFX11_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4938096.1"
                     /translation="MSVTLLYTGLLALIFLTLSARVVLMRGAGGPSLGDGGNPVMLRR
                     IRAHGNFAEYVPFILIMMGALELSGLSTQVIHGLGITLVLARLLHGYALSFTASFKFG
                     RMSGAALTFLLLLVGGGLCLYQALIKLGGSA"
     gene            complement(2016..>2414)
                     /locus_tag="VFX11_05450"
     CDS             complement(2016..>2414)
                     /locus_tag="VFX11_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4938097.1"
                     /translation="GVDDLEASLRFYRNGLGFPTEGIIGKEFEHGAVVFIQLQPGLRL
                     ALWPRNSIGHDTGLAVGPTSPTEMTLGHNVSSKAEVDEVMAKALSAGAVMVKPAHDTF
                     WGGYSGYFQDPDGHLWEIVWNPQWSDEWQA"
BASE COUNT          567 a          710 c          718 g          419 t
ORIGIN      
        1 cgacccatca cccttgtagc ggatcggttg atcggcgatc accgtgaagt catcaaactt
       61 ggcttcaagc cggagtttgt cgagaaagtt gaccttgatt tccatggggg gtaccttgaa
      121 atttgattct gaacgggttt cggtaaggct ggtgttggaa tcaaagtgat cttgtggatg
      181 tcacctggat tctctgccga agtgagtcga gtgttactgc accttgaaat ggtcggcgca
      241 atgtcggcgt ccgtgctcgt tataggcgat ggcaatcagt gggcccagcg agagcactag
      301 aagccccaac ggcagggaca agccttgtgc cacggcgata gggccgagcg cgatgctggc
      361 caggcaaacc agagtcagcc ccagctgctg gttgcgggcg cgggtggcat agcagttcag
      421 cgcccaaagc gagcccacga atattgcctc ggccactgct accgtgctga tggcgtacag
      481 cggcgagact gcatgcacat caaccgccgc atggctggct ttgagcacat ccgctaccac
      541 ctccagtccg gcgccaacgg cagccactgc tgcaaacagg atgtagtggc cgtaacccca
      601 gccccaggcg cgctcgcgat gatgatgcag ggcatccgca gacggcagca ggaagtacat
      661 ccaccacaaa ctgaaaatca gtagcgtgcc ggcaaagccc agcaacgcca gatcgaatga
      721 ccagccttgc gcctgccaca tggtggccac ggtgttggtg gcccccagaa cacattcgcc
      781 caacacaatg atgaccatca ggccgtagcg ctcagcaatg tgatgggcat gccaaggggt
      841 ggtgccggcc ttttcggccc acatgggtac ggccagttcg aatacccaca gaacggcaat
      901 ggcggcccag ggagtagcgc cggtcagcac accattgacg accgccagca gaaagccaac
      961 ccatcccagc tgtaccagaa cgatgccgcc agcatagcgc aggcaggttt tacgccgcag
     1021 cgggtccccg tgcgcagccc gcagccactg tgcgcaaagg gccagacgca tgaccacgta
     1081 accgacaacg ctcgccgtgt actggccggt gaacaggccg ggaatgccgg tcgccagtac
     1141 cagcacgccc cccatctgca tcatggtcag cacacggtac agggtgtcat cgttatcgta
     1201 ggccgacgca aaccacgtgt agttcatcca ggcccaccag atggccagaa acgccagcag
     1261 gtagcccggg agcgtttgca gcacatgatg ctccaccacg ccgtggtgga gctgggcggc
     1321 cgccagggca atggcaacca cgaaggtcag gtcgaacaac agctcaagcg gcgtggaggc
     1381 gcggtgctct tcgtggatgt cacgcccacg aggcgccgct ttcagggtaa tcatggttag
     1441 tcatactcgg gtgtggtggc agcgcagtcg taatgcggtg gcagaaggct caggcactgc
     1501 cacccagttt gatcagcgcc tgatagaggc aaaggccgcc acccaccagc agtaacagga
     1561 acgtgagggc agcaccgctc atgcggccga acttgaatga cgcggtgaac gacagcgcat
     1621 atccatgcag caggcgcgcc agcaccagcg taatgccaag cccatgaatg acctgggtgg
     1681 aaagaccgga cagctccagc gcccccatca tgatcagaat gaaaggaaca tactcagcga
     1741 aattgccgtg tgcccgaatc cggcgcagca tgaccgggtt gccgccgtcg cccagactgg
     1801 ggccgcccgc gccgcgcatc agaacaacac gagcgctcaa tgtcaggaaa atgagtgcaa
     1861 gaaggcctgt gtacagcagt gtcacggaca tggagattcc tcgtgtgggt tttacgcacc
     1921 ccaggcgcct ttcaaatcag ggggcagggg ggcagatgga ggggattata agcgtggtta
     1981 tgctctggct gcagaacatg ctggaggttc gccccttaag cctgccattc atctgaccat
     2041 tgcgggttcc agacgatctc ccagaggtgg ccatcggggt cctggaagta gccagaatag
     2101 ccaccccaaa aggtgtcgtg agcgggtttt accatgacag cacctgcaga aagggcttta
     2161 gccataacct catcaacttc cgcttttgag gaaacattgt gacctaatgt catttcggta
     2221 ggactggttg ggcctacggc aagccccgtg tcatgaccaa tgctgttgcg aggccacagg
     2281 gccaaacgca aaccaggctg aagctgaatg aatacaacag ctccatgctc aaactccttg
     2341 ccgatgatgc cttcagtagg aaacccaagg ccgttacggt agaaacgcaa ggatgcctca
     2401 aggtcatcaa cgcc
//
LOCUS       DASUEG010000295         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_919327, whole genome shotgun sequence.
ACCESSION   DASUEG010000295 DASUEG010000000
VERSION     DASUEG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919327"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFX11_05455"
     CDS             <1..690
                     /locus_tag="VFX11_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4938098.1"
                     /translation="VHMNMVTGSGVLLELVEPEKMRPEYVPVNIEIEAALPADEEDAV
                     RQRILDAARDLYIENGLRRTTMEDVAKRAGMGRATLYRRFSEKDQLFQALVLRDVQRD
                     LVLIENAIRSISSALDGLLEAFVQACGLTHRNALLSHLLNKEPDHVLPYLTSGFEVIM
                     QFSRIYLAGQITRAQQAGHIRDLPADMTAEMIMRMMHSMMLSPRGVIDPGDEVSLRRF
                     AELYLRPLLTP"
     gene            810..1745
                     /locus_tag="VFX11_05460"
     CDS             810..1745
                     /locus_tag="VFX11_05460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4938099.1"
                     /translation="MSLTAQADSFDSGTTEAETPVSRRLDLRPLGPDSCTWRDFGSYL
                     FHLMLPQAFVLQSAHPVIDAAVSKEKKYKLDPWGRAKGSVALLWPVVYSRPEKAIAMG
                     VKLREMHRQIKGVDKDGKAYHALDPEAYSWVHITGFDASVRMFELFGKPVSAEERARM
                     FAEWKQMGALLGIIDKHIPQTEEEYWKHFNYIIEHRLKYGEVLEDLLSPSFYAGYPKP
                     PEMPWMPDLLWKAIATPASWFAHRLTIATLPVNFREKFKLRYNKVDQLLFRAFALMVR
                     VFYPLTPENLRYIPLARRAINDARRHPEAYQLNNI"
     gene            1842..>2540
                     /locus_tag="VFX11_05465"
     CDS             1842..>2540
                     /locus_tag="VFX11_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938100.1"
                     /translation="MTHPKPWLATYEALGQDWFNIPELPEKTLSDYVRDNARDYGERT
                     ALVYFGVRTSYALLDLQADQLANVLRAQGCKAGDVLAIQLPNSPQYVLGMVAAARLGM
                     VVTSVSALLTPPEVIAQSIDANVKALLTLDALWGAMGDKLTQAIPSLQLAIVSTMTEM
                     LPGMPNATPANAKVGNVTVVGLLEALQTASTERVTDPVSIDRVLYLQYTGGTTGKAKG
                     AQLTSRNLFINNVQA"
BASE COUNT          525 a          753 c          747 g          515 t
ORIGIN      
        1 gtccacatga acatggtcac cggtagcggg gttttgctag agttggtgga accagaaaaa
       61 atgcgtccgg aatacgtgcc tgtgaatatt gaaatcgaag ctgccctgcc tgccgacgaa
      121 gaggacgctg tccgccagcg catcctggac gcggcccgcg atctgtatat tgagaatggc
      181 ctgcgccgca ccaccatgga agatgtggcc aagcgtgccg gcatggggcg cgccaccctg
      241 taccgccgtt tcagcgagaa ggaccagctg ttccaggccc tggtgctgcg cgacgtacag
      301 cgggatctgg tgctgatcga gaacgccatc cgtagcatct cgtcagccct ggacggcctg
      361 ctggaagcct ttgtccaggc ctgcggtctg acccaccgca acgccctgct gtcccacctg
      421 ctgaacaagg aaccggatca cgtgctgccg tacctgacat cgggctttga ggtgatcatg
      481 cagttttccc gtatctacct ggcgggacag atcacccggg cccagcaggc cggccacatc
      541 cgggaccttc ccgccgacat gaccgccgaa atgatcatgc ggatgatgca ctccatgatg
      601 ctgagcccgc ggggcgtcat tgatcctggt gatgaggtca gcctgcggcg cttcgctgaa
      661 ctgtatctga ggccgctgct cacgccctga ccgctcgcac cctgaccgga acggttacgt
      721 cagacgtaca ttttggctat tgcattttgc cgtgataaga catatttttg attttgtctc
      781 atccgcaata gtcaaaaggg gctgcgtcca tgtcgttaac tgctcaagcc gattcgtttg
      841 attcaggcac aaccgaagct gaaaccccgg ttagccgtcg gctcgacctc cgcccgctgg
      901 gtccggattc ctgcacctgg cgggacttcg gttcctacct gttccacctg atgctgcccc
      961 aggcgtttgt cctgcagtcg gcccatccgg tgatcgacgc tgccgtcagc aaggaaaaga
     1021 aatacaaact ggacccctgg ggccgggcca agggctcggt ggcgctgctg tggccggtag
     1081 tctattcgcg accggaaaag gccattgcca tgggcgtgaa actgcgggaa atgcaccgcc
     1141 agatcaaggg cgtggacaag gacggcaagg cctatcacgc gctggacccg gaagcctatt
     1201 cctgggtgca catcaccggt ttcgacgcgt cggtgcgcat gttcgaactg ttcggcaagc
     1261 cggtgtcggc cgaagagcgg gcccgcatgt tcgccgagtg gaagcagatg ggcgcgctgc
     1321 tgggcatcat cgacaagcac atcccacaga ccgaagaaga atactggaag catttcaact
     1381 acatcattga acaccgtctg aaatacggcg aggtgctgga ggatctgctc agcccgtcct
     1441 tctacgccgg ctatcccaaa ccgccggaaa tgccctggat gccggatttg ctgtggaagg
     1501 ccattgccac gcccgccagc tggtttgcgc acaggcttac cattgccacc ctgccagtga
     1561 atttccgcga aaaattcaaa ttgcgttata acaaggtcga tcaactgctg ttccgcgcct
     1621 tcgctttgat ggtgcgggtg ttctatccgc tcacacccga aaatctgcgc tacattccat
     1681 tggcacggcg cgcgatcaac gatgcacgga gacacccaga ggcataccag ctcaacaaca
     1741 tataactaat aagctttttt aagtgcctgg ttcgaggata accggcgtgt cgcgcgccgg
     1801 attttttccc acaggctttt gttcagacag agggtagaaa catgacacat ccaaagccgt
     1861 ggctggccac atacgaggcg ttgggccagg actggttcaa tatccccgaa ctgccggaaa
     1921 aaaccttgtc cgattatgtg cgcgacaacg cgcgcgatta cggcgaacgc accgcgctgg
     1981 tgtatttcgg cgtgcgtacc agctatgcgc tgctggatct gcaggccgac cagctggcca
     2041 atgtgctgcg cgcgcaaggc tgcaaggccg gggatgtgct ggctatccag ctgcccaatt
     2101 caccgcagta tgtgctgggc atggtggcgg cagcgcgtct gggcatggtg gtcaccagtg
     2161 tgtcggcgtt gctgactccg ccggaagtga ttgcacagtc catcgacgcc aatgtgaagg
     2221 cactgctgac gctggatgcg ttgtggggtg cgatgggtga caaactgacg caggcaattc
     2281 cgtcgctgca gctggccatt gtgtccacca tgacggaaat gctgcccggc atgccaaatg
     2341 ccacgcccgc aaatgcgaaa gtgggcaatg tcacggtggt cggcctgctg gaagcgctgc
     2401 aaaccgcgtc caccgaacgg gtcactgatc ccgtctcgat cgaccgtgtg ctgtatctgc
     2461 aatacaccgg tggtaccact ggcaaggcca agggcgcgca actgacgtcg cgcaacctgt
     2521 tcatcaacaa tgtgcaggcc
//
LOCUS       DASUEG010000296         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1363572, whole genome shotgun sequence.
ACCESSION   DASUEG010000296 DASUEG010000000
VERSION     DASUEG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363572"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VFX11_05470"
     CDS             <1..1273
                     /locus_tag="VFX11_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX4938101.1"
                     /translation="GTNGLVYEQMIIDLPVFTEREQELLPLYTSLVSELGAGDNDYMA
                     MQQWQSRVSGGLRMSLSVRTDLHDAGKGLGHLVFSGKALADKATDFNQLMLATLDGLR
                     FDEGSRVRELVSQMRARWEASVTGQGHGLAMQTAGSAFSAVAAFNQRISGLPGIRRMK
                     ALDSALAEDTTLQQLLSDLQGMHRKVQQSQRRFLLIGEEERLPALESSLQDAWQGAVW
                     QPQGGTTVFAAPPSARNVAWLCNTQVQFCARAFPAVPVEHPDSAALMLLGGFLRNGYL
                     HNAIREKGGAYGGGAGYDGNACAFRFYSYRDPRLTDTLKDFDASIDWLLNEAHEQRQL
                     EETVLGVISDMDKPLSPAGEARQAYHNALYGRTHEQRKRLRERLLAVSLKDLQRVGDT
                     WLAKGMACTAVIAPNARAAEVESLGLAIEKL"
     gene            1277..2074
                     /locus_tag="VFX11_05475"
     CDS             1277..2074
                     /locus_tag="VFX11_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938102.1"
                     /translation="MSKPELHFAHANGIPSACYRKMLGALDASFAVKAVPVLGTDPNY
                     PVDNNWHSLAEQVADSIRVRCNGPVIGVGHSLGGLTTFMAAHRYPELFRAVVIMDPPV
                     INGLGALSFGFMKALGQVDRVTPAGKSKGRREVWPSREVAHELLGKKRLFRSFDPDCF
                     EDYIRFGLSDCEEGVCLTIPAAVEVAIFRNTPHDSWRYRRPLQVPGILLSGAGSEFRG
                     TGFAERMAHMHGMSHEYVEGGHMFPLEKPLQTADHILAALRQRKVIT"
     gene            2080..2925
                     /locus_tag="VFX11_05480"
     CDS             2080..2925
                     /locus_tag="VFX11_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938103.1"
                     /translation="MVERSFLHKGRRIAARCWHDPSLPPLLALHGWQDNAATFDRLAP
                     LLSGFHVVAMDFAGHGFSDWRAAGMRYHTMDHVDDVLAVVSQLEWDRFALVGHSMGAG
                     VGSLFAGAMPERVSRFVMIDGLGPYAGRAEDAPAILREALLEWQAFESRPDRVFPDRE
                     TAVLARQRGFTPLSKEAAEILCSRSLKEVEGGFAWTMDRRIRHHSSLRLTESQVRAFL
                     AAIEADVMVIRAEQGFPADPAVFDGRLAALKRLELQQVKGAHHLHLDESPYEVAALIN
                     RFLHG"
     gene            complement(2933..>3571)
                     /locus_tag="VFX11_05485"
     CDS             complement(2933..>3571)
                     /locus_tag="VFX11_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4938104.1"
                     /translation="VTRDADHQAALSWVTSHWQGLDILVNNAGVAGGGDFEGISEEDW
                     LWMLNINLLGVVRGCRAFTPLFKQQQGGQFVNIASMAGLLNPPAMANYNVAKAGVVAL
                     SETLAAELSPWKIRTLAVCPSYFQTNLAESLRTHDPLTKAALGKLLASSDLTADDIAE
                     GIVKAVETGENLYLPHEPARQAWQFKREHPGVIAEDMLAYARKLAMVRSNNT"
BASE COUNT          637 a         1008 c         1151 g          775 t
ORIGIN      
        1 gggtacgaac gggttggtct atgaacagat gatcatcgac ctgcctgtat tcacggagcg
       61 ggagcaggaa ctgctgccct tgtacacatc gctggtcagc gagctgggtg ctggcgacaa
      121 cgattatatg gccatgcagc agtggcagtc gcgtgtatct ggcggattgc gcatgagcct
      181 gtccgtgcgc accgacctgc acgatgctgg caagggtctg gggcatctgg tgttttccgg
      241 caaggcgctg gctgacaagg cgactgactt caaccagctg atgcttgcca cgcttgatgg
      301 ccttcgcttt gacgaaggat cacgtgtacg cgagctcgtt tcgcagatgc gtgcccgctg
      361 ggaggccagc gtgacggggc agggacatgg tctggccatg cagacagccg gatccgcctt
      421 cagtgccgtc gctgctttta atcagcgtat ttccggcttg ccgggcattc gccgcatgaa
      481 ggcgctggac agtgcgcttg ctgaagatac aaccttgcag caactgttat cagacttgca
      541 gggcatgcat cgcaaggtgc aacagtcgca gcgccgcttc ctgctgattg gtgaggaaga
      601 gcgtttgcct gcactggaat cttctctgca ggatgcctgg cagggtgctg tctggcagcc
      661 acagggcggc acaaccgttt ttgcagcacc accgtcagcg cgcaatgtcg cctggctatg
      721 caatacgcag gtgcagttct gtgcgcgcgc tttccccgcc gtgccggtcg agcatcccga
      781 ttcggccgca ctcatgctgc tgggcggatt cctgcgcaac ggctatctgc acaacgcgat
      841 ccgtgaaaag ggtggtgcct atggcggtgg tgccggctat gacggcaatg cctgtgcctt
      901 ccgtttctat tcctatcgtg atccgcgcct gacggacacg ctgaaggatt tcgacgccag
      961 catcgactgg ctgctgaatg aagcacacga gcagcgtcag ctggaagaga cagtgctggg
     1021 cgtgatttcc gacatggaca agccgctgtc tccggcaggg gaagcgcgac aggcgtatca
     1081 caatgcgctc tacggtcgta cgcatgagca gcgcaagcgc ttgcgcgagc gtctgctggc
     1141 tgtttcgctg aaagacctgc aacgtgtcgg tgatacctgg ctggccaagg gaatggcttg
     1201 cacggcagtg attgccccca acgccagagc cgcggaagtg gagtcgctcg gtctggccat
     1261 cgagaagctg taacccatga gcaaacccga actccacttt gcccacgcca acggcattcc
     1321 ttcggcctgc tatcgcaaga tgctgggcgc gctggatgca tcgtttgccg tcaaggcggt
     1381 gcctgtattg ggcactgatc cgaattatcc cgtggacaac aactggcatt cgctggcgga
     1441 gcaggtggcc gactccattc gcgtgcgctg caacggcccc gtgatcgggg tagggcactc
     1501 gttgggagga ctcaccacgt tcatggcggc gcatcgttac cccgaactgt tccgagccgt
     1561 cgtcatcatg gatccgccgg tgatcaacgg tctgggcgcg ctcagtttcg gtttcatgaa
     1621 agccctcgga caggtggacc gggtgacacc ggcaggcaag agcaaagggc gccgcgaggt
     1681 ctggccatca cgcgaggtgg cgcatgagct gctgggaaaa aagcgcctgt tccgcagttt
     1741 tgatccggac tgtttcgagg actatatccg cttcggtctg agtgactgtg aagagggtgt
     1801 atgcctgacc attccggcgg ccgttgaggt ggcaatattc cgcaatacgc cgcatgactc
     1861 atggcgctat cgtcgtcctc tgcaggtgcc tggcattctg ctttcggggg ccggtagtga
     1921 gtttcgcggt accggttttg ccgaacgcat ggcgcacatg catgggatgt cgcatgagta
     1981 tgttgaaggc gggcatatgt tcccgctgga aaagcccttg caaacggctg accacatcct
     2041 ggcagcactc cggcaacgga aggtgattac gtgacacagg tggttgaacg cagcttcctg
     2101 cacaagggac ggcgcattgc agcccgttgc tggcacgatc cttccttgcc accgctgctg
     2161 gcgctacatg gctggcagga caatgccgcc acgtttgacc ggttagcgcc gttgttgtcc
     2221 ggctttcatg tggtggcgat ggattttgcc gggcatggct tcagcgattg gcgtgcggca
     2281 ggtatgcgtt atcacaccat ggatcacgta gatgacgtgc tggccgtggt gagccagttg
     2341 gagtgggacc gatttgcctt ggtggggcac tccatgggtg ctggcgtcgg ctcgctgttt
     2401 gccggcgcca tgccggagcg ggtcagtcgc tttgtgatga ttgatggcct cgggccttat
     2461 gcgggccgtg cggaagatgc gcctgccatc ctgcgcgaag ccttgctgga gtggcaggcc
     2521 ttcgagtcac gccccgatcg ggtattcccg gatcgtgaga cggccgtgtt ggcgcgtcag
     2581 cgcggattta cgccgctcag caaagaggca gccgaaatcc tttgttcgcg ttcgctgaaa
     2641 gaagtggagg gcggttttgc gtggaccatg gatcgccgta tccgtcacca ctcgtcattg
     2701 cgcctgaccg agtctcaagt gcgggccttt ttggcagcca ttgaggctga tgtgatggtg
     2761 atccgggccg agcagggatt cccggccgat ccggccgttt ttgatggtcg gctggcggcg
     2821 ttgaaacggt tggagttgca gcaggtgaag ggcgctcatc acctgcatct ggacgagagt
     2881 ccatatgagg ttgcggccct catcaatcgt ttcctgcatg gctgacgggg tttcaggtgt
     2941 tgttactgcg taccatggcc agcttgcgtg cataggccag catgtcctcg gcaatcacgc
     3001 cggggtgttc ccgtttgaat tgccatgcct gtcttgccgg ttcatgcggc agatacaggt
     3061 tttcaccggt ctccaccgcc ttcacgatgc cttcggcaat gtcatccgcc gtgaggtcgg
     3121 agctggccag cagtttcccc agcgcggcct tggtcagtgg atcgtgcgta cggagggatt
     3181 cagccagatt ggtttgaaag taagacgggc agacggccag cgtgcgaatc ttccagggcg
     3241 aaagttccgc cgccagtgtt tcggagagcg cgaccacgcc agccttggcg acattgtagt
     3301 tggccatggc cggcggattg agaagccccg ccatcgacgc gatgttaacg aactggccgc
     3361 cctgctgttg cttgaacagg ggagtaaacg cacggcagcc acgcaccacg cccaacaggt
     3421 tgatgttgag catccacaac cagtcttctt cgctgatgcc ctcgaagtcg ccaccaccag
     3481 ccacgccggc gttgttcacc agtatgtcca ggccttgcca gtgcgatgtc acccaggaca
     3541 gggcggcctg atgatcggcg tcgcgcgtca c
//
LOCUS       DASUEG010000297         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2124643, whole genome shotgun sequence.
ACCESSION   DASUEG010000297 DASUEG010000000
VERSION     DASUEG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124643"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFX11_05490"
     CDS             <1..366
                     /locus_tag="VFX11_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEX4938105.1"
                     /translation="SRFPAGVPATRCGQGAALVDGVHYFSWIGSATMTNLLDPTNYFP
                     VLDWLNRSISKQVTGVTEITDSVVGQCESHLGKVIRDDYRMNHFDEVNQAFGLVDVFA
                     VNPKTVWKQHANRLKNMGL"
     gene            475..1467
                     /locus_tag="VFX11_05495"
     CDS             475..1467
                     /locus_tag="VFX11_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEX4938106.1"
                     /translation="MAWLIGPGFFWPEWSAPDQPAAARRQGSHFPAPAYGAPVLPGSA
                     DTAPTFTTGLENLPAFLQDTSVEDHLHVDTAGNLIVDAAARNLFDYFLSLNGLESQAV
                     IEQRIQAYLQHRLPPPAAAQAEALLQQYLAYKLALHSMQEAEVVAGEPVDIASVRTQM
                     ALDKAAREQHFSAPEAEAFFQAEDAFNADALARLDIVTDPDLDATTRTERLAQLDTAL
                     PAEQQQARAEAAQLQQLLALAAQTQNGSENAENLQQMRMALAGPEAAERLQALDQERQ
                     AWERRVREWQQERSRILEQTGLAPEDAQAALDQLRNERFDPVERLRLSGIEADR"
     gene            complement(1515..2252)
                     /locus_tag="VFX11_05500"
     CDS             complement(1515..2252)
                     /locus_tag="VFX11_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4938107.1"
                     /translation="MPLLFGLSILVQILLVIHIMKTGRNNYWIYLVIFAPLIGSVAYL
                     ILELLPEFFATRTGRKLSRNLDNAINPHRDLKKAALNLEIADTVENARRLAQQCLDSG
                     RFADARDLFRKSLNGLYHDDPNLMLGLAQSQFGLGEFQQVKNTLDELKQKNPDYRHAD
                     AHLLYARALESLGDTAAALHEYEVLADYYPGPEPVVRLGLLLQQLGRGQEAQDRFTRV
                     LTLAKNSGRNYRDFHKEWIAIAKRETK"
     gene            2389..3423
                     /locus_tag="VFX11_05505"
     CDS             2389..3423
                     /locus_tag="VFX11_05505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008117.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEX4938108.1"
                     /translation="MSKAKAERKIYGINACQSFYQRFPERIIRAWITEEAAKISFGPL
                     MKFLAQEKRAYHLADAAELERVTESSHHEGVCLLVQELPVFTPESWLAGLKPGKPLCV
                     LALDGVSNPHNLGAIMRVAAHFGVSAILTTNPQALRSGSAMRTAEGGATHVMVMGTDN
                     LVKTLNLLKKNEFTVLATSSHESRSLYDAPLPARLVLVMGEEQTGVSKAVLQVVDGRI
                     AITGTSWVESLNVSVATGVLLSEFWRQHRIARREPAPEHAATTTTAEAPRRTAGARPG
                     GGARQERRPGDRTRKSGPRPAGGKASGKPSGKSSGKPSGKPAGKGGFKPKPAGGKAGA
                     RKAGGKPKRG"
     gene            complement(3434..4018)
                     /locus_tag="VFX11_05510"
     CDS             complement(3434..4018)
                     /locus_tag="VFX11_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4938109.1"
                     /translation="MNVLIPIAAGALLLAGTPVALAQQDRTGSLYLGATFQKIALESD
                     GLYDSLALDDTDSDYAVFGGIRFSRTFALEIDYAPLGEFAYLGGSVEYSGAQVAGLMV
                     APVDTELELFARGGVGLVEVDQSAPAFNDTSTAFSLGVGADWTPRDLDAITVRVLIST
                     RWIRVEPRDAQPGDPGQRYDQNLYEYGVGMAYNF"
     gene            complement(4058..>4761)
                     /locus_tag="VFX11_05515"
     CDS             complement(4058..>4761)
                     /locus_tag="VFX11_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX4938110.1"
                     /translation="EETGAQAVVRACMAFSAEVASREDVAPAAPPAPVASAPSPVAVS
                     SGLVERQTAPPDDATLITPELTSPFRDEDETPVLVDLPGAFAAPPPLNSPRRALPRWP
                     LLALAVPVFALLAAVPWLWPLTQVPGAHLVAVGETLNLAQPYQQQVDAFWQRHGRPPA
                     NLSELNLAAPPALGEQATLTLGGEGRLLIIFTAAAGAIAGTTLELTPAPVDAGIEWRC
                     GGGTLPAVERPAECQ"
BASE COUNT          922 a         1502 c         1534 g          803 t
ORIGIN      
        1 agccgctttc ccgccggcgt gccggccacc cgttgcggtc agggcgccgc actggtcgat
       61 ggcgtgcatt atttttcctg gatcggcagc gccaccatga ccaacctact cgatcccacc
      121 aattattttc ccgtgctgga ctggctcaac cgctccatca gcaagcaggt taccggcgtc
      181 accgaaatca ccgactcggt ggtgggccag tgtgaatccc atctgggcaa ggtgatccgc
      241 gacgactacc gcatgaatca cttcgacgaa gtgaatcagg cgttcggtct ggtggacgtg
      301 tttgccgtca atcccaaaac tgtgtggaaa cagcacgcca accggctgaa gaacatgggc
      361 ctgtaaatca tggtgcagaa cattccacag aatatttcac agaactacag agggaccggg
      421 cacaaaaaga tcggacgcac acgcattctg atcccgcttg tgccggttgt agtggtggcc
      481 tggttgatcg ggccgggctt tttctggccg gaatggtccg cgcccgatca accagcagcc
      541 gcccggcggc agggcagtca tttccctgcg ccggcttacg gcgcgcccgt actgcctggc
      601 agcgccgaca cagcgccaac gtttaccacg ggcctggaaa acctgcctgc ttttctgcag
      661 gacacatcgg tagaagacca tctgcacgtg gacactgccg gcaatctgat agtcgatgcc
      721 gcggcgcgca acctgttcga ttattttctg tcgctcaacg gactggaatc gcaggcggta
      781 atcgaacagc gaattcaggc ttatcttcag catcgtctgc cgccacctgc cgctgcccag
      841 gcggaagcgc tgcttcagca atacctggcc tacaagctcg cgctgcacag catgcaggaa
      901 gcggaagtag ttgcgggaga accggtagac atcgcatcgg tgcgcacgca gatggcgctg
      961 gataaagccg cccgtgaaca gcatttttcc gcgccggaag ccgaggcttt ttttcaggct
     1021 gaagacgcgt tcaatgccga tgcactggcg cggctggaca ttgtcaccga tcccgatctg
     1081 gatgccacca cccggaccga gcggctggct caactggaca ctgctctgcc cgcagagcaa
     1141 caacaggcca gggccgaggc agcgcaactg cagcagttgc tggcgcttgc cgcgcagaca
     1201 caaaacggca gtgaaaatgc ggaaaacctg cagcagatgc gtatggctct ggcgggcccc
     1261 gaggccgccg aacggctgca ggcactggat caggaacggc aggcatggga acggcgggtg
     1321 cgggagtggc agcaggaacg ttcgcgcatc cttgaacaaa cgggcctggc gccggaggat
     1381 gcgcaggcag cactggacca gctgcgcaat gaacggtttg atccggtgga gcggttgcgg
     1441 ttgtcgggaa tcgaggccga ccgctgatcc agcacgctgc aacaccgcac aacgggatgg
     1501 gccggcaccc gacgctactt cgtttcccgc ttcgcgatgg cgatccactc cttgtgaaaa
     1561 tcgcgataat tgcggccgga gtttttcgcc agcgtcagca cgcgggtaaa acggtcctgc
     1621 gcttcctgtc cacgtcccag ctgctgcaac agcagaccca atcgcaccac cggctccggg
     1681 ccgggataat agtccgccag cacttcgtat tcatgcagcg cggcggccgt gtcgcccagg
     1741 ctttccagcg cccgcgcata cagcaggtgc gcatcggcgt gccggtagtc aggatttttc
     1801 tgcttgagtt cgtcgagcgt atttttcacc tgctggaatt cgcccaggcc gaactggctt
     1861 tgcgccagcc ccagcatcag gttgggatcg tcgtgataga gcccgttcag gcttttgcga
     1921 aacagatcac gggcatcggc aaagcggccg ctgtccaggc actgctgggc cagccggcgc
     1981 gcgttttcca ccgtgtcggc gatctccaga ttgagcgcag cctttttcag atcccggtgc
     2041 gggttgatgg cgttgtccag gttcctgctg agcttgcgtc cggtgcgggt ggcaaaaaat
     2101 tccggcagca gttccaggat caggtaagcc acgctgccga tcaggggcgc gaagatcacc
     2161 agatagatcc agtaattgtt acggccggtt ttcatgatgt gaatgaccag caggatctgc
     2221 accaggatcg acaggccgaa cagaaggggc atgggagtgt cctcaatcgt atgttgcgcg
     2281 cggtgaacgt ctgctcttca gtttaacagg cgcctgtgac ggccggccgt ggacccgcag
     2341 gctctgtgat agaattcgcg ctttccagcc agccggaacc cgcccgccat gtccaaagcc
     2401 aaagccgaac gcaagatcta cggcatcaac gcctgccaga gtttttacca gcgctttccc
     2461 gaacgcatca tccgcgcctg gatcaccgag gaggcggcca agatcagctt tggcccgctg
     2521 atgaaattcc tggcgcagga aaaacgcgcc taccatctgg cggacgcggc ggaactggag
     2581 cgggtcactg aatcgtccca ccacgagggc gtgtgcctgc tggtgcagga actgccggtg
     2641 ttcacgccgg aatcctggct ggcggggctg aagccgggca agccgctgtg cgtgctggcg
     2701 ctggacggcg tcagcaatcc ccacaacctg ggggcgatca tgcgggtggc ggcgcatttc
     2761 ggtgtgagcg cgatcctgac gacaaatccg caggccctgc gctccggctc cgccatgcgc
     2821 accgccgagg gtggcgccac ccatgtgatg gtgatgggca ccgacaatct ggtgaaaacc
     2881 ctgaatctgc tgaagaaaaa cgagttcacc gtgctggcca cgtccagtca tgaaagccgc
     2941 tcgctgtacg acgcccccct gccggcacgg ctggtgctgg tgatgggaga ggaacagacc
     3001 ggcgtcagca aggcggtgct gcaggtggtg gacggccgta tcgccattac cggaaccagc
     3061 tgggtggaaa gcctgaacgt gagtgtcgcc accggtgtgc tgctgagcga attctggcgt
     3121 cagcaccgca tagcacgccg tgagccggcg ccggaacacg cggccacgac cacgacggct
     3181 gaagcaccac gacgtacagc aggtgccagg cccggaggtg gcgccaggca ggagcgccgg
     3241 cccggtgatc gcacacgcaa atcgggacca cgccccgcag gcggcaaagc ctctggcaag
     3301 ccgtcaggaa agtcatctgg aaagccctcc ggcaagcccg caggcaaagg cggtttcaaa
     3361 ccaaaaccgg cggggggcaa ggccggggcg agaaaagcgg gcggcaaacc gaagcggggc
     3421 tgaaggccgg ccctcagaag ttgtaggcca tccccacccc gtattcgtac aggttctggt
     3481 cgtaacgctg gccgggatcg cccggctggg catcgcgggg ttccacccgg atccagcggg
     3541 tactgatcag cacccgcacg gtaatggcat ccaggtcgcg cggcgtccag tcggcgccca
     3601 ctcccaggga gaaggcagtg ctggtgtcgt tgaaggccgg cgctgactgg tccacttcca
     3661 ccagccccac accgccccgg gcgaacagtt ccagttcggt atccaccggc gcaaccatca
     3721 ggccggccac ctgggcccca ctgtattcca cgctgccgcc caggtaggca aattccccca
     3781 gcggggcgta atcgatttcc agggcgaagg tccggctgaa gcggatgccg ccgaataccg
     3841 cgtaatcgga atcagtgtcg tccagcgcga ggctgtcgta caggccgtcg ctttcaaggg
     3901 cgattttctg gaaggtggcg ccgaggtaca ggctgccggt gcgatcctgc tgggccagcg
     3961 caacaggggt gccagccagc agcagggcgc cggccgcaat cgggatcaga acgttcatgc
     4021 gatcaccagc tgattgattt cattgcagta tagcgaacta ctggcattcc gccgggcgct
     4081 cgacggccgg cagcgtgccg ccgccgcacc gccactcgat gccggcatcg accggggccg
     4141 gggtcagttc cagggtggtg ccggcaatgg cgcccgcggc agcggtgaag atgatcagca
     4201 ggcggccttc accacccagg gtcagcgtgg cctgttcacc caacgccggc ggtgccgcca
     4261 ggttcagttc ggacaggttg gccggcggcc ggccgtgccg ttgccagaac gcgtccacct
     4321 gctgctggta gggttgggcc agattgaggg tttcgcccac cgccaccaga tgagcgccag
     4381 gcacctgggt cagcggccac agccagggca cggccgccag cagggcgaag accggcacgg
     4441 ccagggccag caggggccag cgcggcagcg cacgccgtgg cgaattcagg gggggcggtg
     4501 ccgcaaacgc cccgggcagg tccaccagca cgggcgtttc atcctcgtca cgaaagggcg
     4561 atgtcagctc cggcgtgatg agcgtggcgt catcgggcgg cgcagtctgg cgttcgacca
     4621 gcccgctgga aacggccacc ggcgaaggtg cgctggcgac cggtgccgga ggtgctgccg
     4681 gcgccacgtc ctctcttgac gccacttccg cactgaacgc catgcaggcc cgcaccactg
     4741 cctgggcacc ggtttcctcc a
//
LOCUS       DASUEG010000298         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_653968, whole genome shotgun sequence.
ACCESSION   DASUEG010000298 DASUEG010000000
VERSION     DASUEG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653968"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3930)
                     /locus_tag="VFX11_05520"
     CDS             complement(<1..>3930)
                     /locus_tag="VFX11_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4938111.1"
                     /translation="QIPYIIDLQTLADDKSAKDFFAHQRDYRRAIRDYQQISLSAQNR
                     FMPAGRIAFQFNYFNFLRDVEVCGYRSFPYTFSSHVDNTVQIFVKDYGDDGAVELWFD
                     GSVFVPLDFIDRMVALVEQFAATGDLKFGELEYCLPLERDAEAVWNDTASQVQAPATL
                     VHWFEEAVSRFGSNVAALYRDTVLTYDELNRQANRLARLLRANNLQRGERVGICHGRS
                     TDLLVAVWAVLKAGGTYIPIEASYPQERIQYILRDSGARLLLTEKCIAKRLPDYAGTA
                     LLLDAMADELAQFDAANLDDKPSGDDGIYIIYTSGSTGNPKGAALHHAGEVNLQQWYT
                     QACQFGPADRTILVSAFGFDLTQKNLFGPLLCGGAVVIPAMDEYDAEVVAADIARTQA
                     THINCAPSAFYALVEHLDEARARQLQSLRWVYLGGEPIRMNALYNWLQHPTCKAQIVN
                     SYGPTECTDVVSWHVIDDISPTAAGVPIGRPICNTALHILNDALRPVPHGVVGEICIT
                     GAGVGLGYVNRDDLTAQVFVDNPFGAGKLYRTGDLGRFMPDGNIEYIGRKDFQVKVRG
                     LRIELGEIEHAIKQLPGVTDALVMVKQDRLIGYAVSAQSTSALNWQAPLRNSLPDYMV
                     PARLILLPAWPLTPNGKIDRKALPDPDIQQRSMPYAAPRDDIEIAIADIWSQLLKKDE
                     IGINDSFFDLGGHSLLANQIVSRLRKHFAVALPIRDLMVHPTVAELAQRVRMAQKNQQ
                     QAALTVCDRNQRIPLSIPQQRLWLLDQIEPGNPAYHVPSIIRVSGALDAQVLEQAFAA
                     VVNRHEGLRAIFTEDEQGPQQIIQPQQAWKIQHQVLAANADEAAIRKQVAAAVLKPFD
                     LAQGPLFRAAVLEQGANQHILVVVLHHIVTDGWSNGILVRDLAQAYAQLKSTGRLQFA
                     PLPLQYADFAAWQRTVLSQENLAQKIAFWRDRLADVPALELPTDFRRPPVQTFHGGSV
                     RLELSADTAEKLQLLGAESQSTMFMVMLAAYASLLQKYSGQDDFCVGTPVAGRDRPEL
                     ESIVGFFVNTLALRVTPQPTMSFRQLLDAVRESALSSFEHQDVPFEQIVEDINPVRDM
                     SRSPLFQVMLVYQNIPVDNDTGIQPTQLGDIELAPFNLDIETAKFEQTLTLWPQHNAI
                     GGSLTYNTDLFEAETAQRFARQFERLCAAVAANPDRALYEFNVLDRQEIQTQLVEWNQ
                     TARTYDRELCVHALIHQASQRHANGTAVRCGDEALTHAELDRLSNRVAHQLLANGVGP
                     GDLVGVALDRNLHLMTAILGILKAGAAYVPIDAAYPEDRIRYILEQAR"
BASE COUNT          819 a         1208 c         1145 g          758 t
ORIGIN      
        1 gcgggcctgc tccagaatgt agcggatgcg atcttccgga taagcggcat cgatgggcac
       61 ataggcggca ccggctttga gtatgccgag aatcgcggtc atcaggtgca gattacggtc
      121 cagcgcaaca ccgacaagat cgccggggcc gacaccgttg gccagcaact gatgggcaac
      181 gcgattcgac agccgatcca gctcggcatg cgtcagcgcc tcatcgccac aacgcactgc
      241 ggttccgttg gcgtgacgct gtgacgcctg atgaatcagc gcgtgcacgc agagttcgcg
      301 atcataggtt cgtgccgtct ggttccattc caccagctgg gtctggattt cctgccgatc
      361 cagcacgttg aattcgtaca gcgcccgatc cggattggca gcgacggcag cacacaggcg
      421 ttcgaactgg cgcgcaaaac gctgcgccgt ttccgcttcg aacagatcgg tgttgtaggt
      481 cagcgagccc ccaatggcgt tgtgctgggg ccagagcgtc agggtctgct cgaacttggc
      541 ggtttcaata tccagattga acggtgccag ttcgatgtcg cccagttgtg tgggctgaat
      601 cccggtatcg ttgtccaccg gaatgttctg gtacaccagc atcacctgga acagcggcga
      661 gcgactcatg tcgcgcaccg ggttgatgtc ctcaacgatc tgttcgaacg gcacatcctg
      721 atgttcgaaa ctgctcaggg ctgattcgcg cacggcatcc agcaactggc ggaaactcat
      781 ggtgggttgc ggcgttaccc gcagcgccag ggtgttgacg aagaaaccga cgatggattc
      841 cagttccggc cggtcgcggc ccgccaccgg tgtgcccaca cagaaatcgt cctgaccgga
      901 atatttctgc agcagcgagg cataggccgc cagcatcacc ataaacatgg ttgactgcga
      961 ttcggcaccc agcagctgca gtttctccgc ggtatcagcc gacagttcca gccgcacgct
     1021 gccaccgtgg aaagtctgca ccggtgggcg gcggaaatcc gtgggcagtt ccagcgcagg
     1081 aacatccgcc aggcggtctc gccagaacgc aattttctgc gccaggtttt cctgcgacag
     1141 aacggtacgt tgccaggccg cgaaatcggc atactgcaac ggcaacggcg caaactgcag
     1201 acggccggtg gacttgagct gcgcgtaggc ctgcgccaga tcgcgcacca gaatgccgtt
     1261 ggaccagcca tcggttacga tgtggtgcag caccaccacc agaatatgct ggttggcgcc
     1321 ctgctccagc accgctgccc ggaacagagg cccctgcgcc agatcgaacg gtttcagcac
     1381 tgcagcggcg acctgtttgc gaatggcagc ttcgtcagcg ttggccgcca gtacctgatg
     1441 ctgaattttc cacgcctgtt gcggctggat gatctgctgc ggaccctgct cgtcctcggt
     1501 aaaaatcgcg cgcaatcctt cgtgacgatt caccacggcg gcaaaggcct gctccagcac
     1561 ctgcgcgtcc agtgctccgc tcacacgaat gatgctgggt acgtgatagg caggattgcc
     1621 cggctcgatc tggtcgagca gccacaaacg ctgctgcgga atcgacaatg gaatgcgttg
     1681 attgcgatcg cagaccgtca aggcagcctg ctgctgattc ttctgcgcca tccgcacccg
     1741 ttgggccaat tcggccacgg tcggatgcac catcagatcg cggatcggca acgccaccgc
     1801 aaaatgcttg cgcaggcgcg acacgatctg gttggccagc agtgaatgcc cccccagatc
     1861 gaagaaactg tcatttatac cgatctcgtc ttttttcagc agctgcgacc agatatcagc
     1921 gatggcaatt tcgatatcat ctcggggtgc cgcgtagggc atgctgcgct gctgtatgtc
     1981 cgggtccggc aacgccttgc gatcgatctt gccgttgggc gtgagcggcc aggccggcag
     2041 cagaatcaaa cgcgcgggta ccatgtagtc cggcagcgaa ttgcgcaggg gcgcctgcca
     2101 gttcaacgcc gacgttgact gcgcactcac cgcgtaacca atcagccggt cctgcttcac
     2161 catcaccagg gcgtcggtca cgccgggcag ctgtttgatg gcgtgttcga tttcgcccaa
     2221 ctcgatgcgc aggccacgca ccttgacctg gaaatccttg cgcccgatgt attcgatatt
     2281 gccgtccggc atgaaacggc cgaggtcgcc ggtacgatac agtttgccgg cgccaaacgg
     2341 attatcgaca aacacctgtg cggtcaggtc atcgcggttg acatagccaa ggccaacgcc
     2401 cgcaccggtg atgcagattt cccccaccac gccatgaggt accggccgca acgcatcatt
     2461 gagaatatgc agcgcggtat tgcagatcgg acggccaatc ggcacgccgg cagcagtcgg
     2521 actgatgtcg tcgatcacat gccaggacac gacgtcggta cattccgtcg ggccatagct
     2581 gttcactatc tgcgccttgc aggtcggatg ctgcaaccag ttgtacagcg cattcatgcg
     2641 aatgggctcg ccgcccagat acacccagcg cagggattgc agctgcctgg ctcgcgcttc
     2701 atccaggtgt tccaccagtg cgtagaacgc gctgggtgca cagttgatat gagtagcctg
     2761 agtccgcgca atatcggccg ccaccacttc ggcgtcgtat tcatccattg ccggaatgac
     2821 gacggcgccg ccacacagca acgggccaaa caggtttttc tgcgtcaggt cgaaaccgaa
     2881 agcgctgacc agaatggtgc gatctgccgg gccgaattga caggcttgcg tgtaccactg
     2941 ctgcaaattc acttcaccgg cgtgatgcag cgcggcgcct ttcggattgc cggtggaacc
     3001 cgaggtataa atgatgtaaa tgccatcgtc gccactgggc ttgtcgtcca gattggcggc
     3061 gtcaaactgc gccagttcat cggccatcgc atccagcagc aacgccgtgc cggcataatc
     3121 cggcaaccgt ttggcgatac atttttccgt cagcagcaga cgcgcgccgg aatcgcgcag
     3181 gatgtactgg atgcgctcct gcggatagga ggcttcaatg ggaatatagg tgccaccggc
     3241 cttgagcacg gcccacacag ccaccagcag atcggtggaa cgcccatgac agatgcccac
     3301 ccgctcgcca cgctgcagat tattcgcgcg cagcaaccgc gccagacggt tggcctgacg
     3361 attgagttcg tcgtacgtca gtactgtgtc gcgatacaac gcggcgacat tactgccaaa
     3421 acggctcacc gcctcttcaa accagtgcac cagcgtggca ggcgcctgca cctgcgatgc
     3481 cgtgtcattc cacaccgctt cggcgtcccg ctccagcggc aggcaatatt cgagttcacc
     3541 gaatttcaga tcacccgtcg ccgcgaactg ctccaccagc gccaccatgc gatcgatgaa
     3601 atccagcggt acaaagacgc tgccgtcaaa ccagagctca accgcgccat cgtcaccata
     3661 gtccttcacg aaaatctgga cggtgttatc aacgtgggac gagaacgtat aagggaacga
     3721 acggtaaccg cacacctcaa catcgcgcag aaaattgaaa tagttgaact ggaacgcgat
     3781 gcggccagcc ggcatgaagc ggttctgtgc cgacagcgaa atctgctgat agtcgcggat
     3841 agcacggcgg tagtcccgct ggtgcgcgaa gaaatccttc gcggacttgt catccgccag
     3901 cgtctgcagg tcgatgatgt agggaatctg
//
LOCUS       DASUEG010000299         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_405664, whole genome shotgun sequence.
ACCESSION   DASUEG010000299 DASUEG010000000
VERSION     DASUEG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405664"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFX11_05525"
     CDS             <1..1107
                     /locus_tag="VFX11_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX4938112.1"
                     /translation="FLNQLLAQAPQQVMDQVQQSGLRGLGGAGFPTGRKWQACAEAPG
                     EEALNTGRYVICNADEGEPGTFKDRVLLQHNLDAVLCGMRMCGHAIGARHGILYLRGE
                     YLFLFDAINTRLQQWREQHWLNADFNIEIQLGAGAYVCGEETALLESIEGRRGIPRIK
                     PPFPVRNGLYQQPTVVNNVETFAAAAWLLLSGVDAFRAVGTEQSPGTRLHSVAGDCDK
                     PGIYELPMGASVQDLLQACAARDPALVQLGGPSGVISSPADWQQPLAFEGISPGGSVM
                     VFDASRSHFNIARNFTAFFQQESCGFCTPCRAGTQVLLRDMDCAAQGQQHADLNALAT
                     LINSTSHCGLGQTAGRPVLHWLHMNAAKKNGTCP"
     gene            1104..1832
                     /locus_tag="VFX11_05530"
     CDS             1104..1832
                     /locus_tag="VFX11_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4938113.1"
                     /translation="MTDTPHFFIDQQAIPFQPGQTLMQAAQAAGRYIPHLCFHDGLTP
                     HGSCRVCIVEINGAIRAACTTPATANLQVHSETPALRTQRRQLIELLFAEGNHLCPSC
                     EVSGNCQLQALAYDLGMTHYEFVPLDPHRQNDGSHPELFIEQDRCIFCELCTRSAQQQ
                     DHKTVFGIGGRGADTYLFAQADSSRLADTAITAQDHAAHICPVGCILPKQGSYQIPIG
                     QRVYDEQPIHLRGNHRPDEQDATP"
     gene            1829..2410
                     /locus_tag="VFX11_05535"
     CDS             1829..2410
                     /locus_tag="VFX11_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEX4938114.1"
                     /translation="MSTDSAHAANAHKVNAHKVKVATCSLAGCFGCHMSFLDMDERII
                     PLIDLIQFDRSPLTDIKIIGDCDIGLIEGGLCNEENVHVAQQFRQHCKILVAVGACAI
                     NGGVPALRNRYTAQECLDESYLHGLGVVDAQIPNDAELPLLLPKVVPLQSAVHVDYFL
                     PGCPPPADAFMEIINAVLQKREPQLDYATRRFD"
     gene            2410..>3423
                     /locus_tag="VFX11_05540"
     CDS             2410..>3423
                     /locus_tag="VFX11_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX4938115.1"
                     /translation="MSETRVVIDPVSRVEGHGKVTLLVNDAGIVTQARLHIVEFRGFE
                     KFIQGRPYWELPMVVQRLCGICPVSHHLVAAKAIDQIAGVTALSAPALKLRRLMHFAQ
                     VLQSHALHFFHLASPDLLFGFQSPVEQRNIVAVIKQQPQIAIKGVKIRKFGQQILEAI
                     SGKRIHGMAAIPGGMNAPLTPEKIQPFTQDVQQIIDWCEETVNLSRQLTDQNRDFHLQ
                     FGLIESPFMGLVGKNGELELYDGNLRVIDAKGNILQDQFPCDQYSQLIQEQVKSWSYM
                     KFPFLTRLGSEQGWYRVGPLARINLCDFIPTPRAEQARQQFRQYAGAPAHSPLLFHWA
                     RMIE"
BASE COUNT          700 a         1129 c          973 g          621 t
ORIGIN      
        1 tttctgaacc agctgctggc gcaggcaccg caacaggtga tggatcaggt gcagcaatcc
       61 ggcctgcgcg gtctgggagg cgcgggcttc cccaccgggc gcaaatggca ggcctgcgcg
      121 gaagcaccgg gcgaggaagc actgaacacg ggacgctacg tcatctgcaa cgccgacgaa
      181 ggcgaaccgg gcaccttcaa ggaccgcgtg ctgctgcaac acaatctgga cgccgtgttg
      241 tgcggcatgc gcatgtgcgg ccatgccatc ggcgcgcgcc acggcattct gtatctgcgc
      301 ggcgaatacc tgttcctgtt cgatgccatc aacacccgcc tgcaacaatg gcgcgaacag
      361 cattggctga atgcagattt caatatcgaa atccagctgg gtgctggcgc ctacgtgtgc
      421 ggagaggaaa ccgcactgct ggaatccatc gaaggccggc gcggcattcc ccgcatcaag
      481 ccgccgtttc ccgtgcgcaa tggtctgtat caacagccga ccgtggtgaa caacgtggaa
      541 accttcgccg ctgcggcctg gctattgctg tcgggcgttg acgcctttcg tgcagtcggc
      601 accgaacaat ccccgggtac gcggctgcac agcgtggccg gcgattgcga caagcccggc
      661 atttacgaat tgcccatggg cgccagcgtg caggatctgc tgcaggcctg cgctgcccgc
      721 gatcccgcgc tggtgcaact gggcgggccg tccggcgtca tcagcagccc cgcagactgg
      781 caacaaccgc tggctttcga gggcatctcg cccggcggtt ccgtcatggt gtttgacgca
      841 tcgcgttccc acttcaatat cgcccgcaat ttcaccgctt ttttccagca ggaaagctgc
      901 ggcttctgca cgccctgccg cgccggcacc caggtgctgc tgcgggacat ggattgtgcc
      961 gcgcagggtc agcaacatgc cgatctgaac gcactggcaa ccctgatcaa cagcaccagc
     1021 cactgcggac tggggcaaac cgccggccgc cccgtgctgc actggctgca catgaacgcg
     1081 gcgaaaaaaa acggaacctg cccatgactg acacgccgca ttttttcatc gaccagcagg
     1141 ccattccgtt ccagccgggc cagacactca tgcaggcagc gcaggcggcc ggccgctaca
     1201 ttccccacct gtgtttccat gacgggctga caccccacgg cagctgccgc gtgtgcatcg
     1261 tcgaaatcaa tggcgccatt cgtgccgcct gcaccacgcc cgccaccgcc aacctgcagg
     1321 tacacagcga aacgccggcg ctgcgcacac aacgccgcca gctgatcgaa ctgctgttcg
     1381 cggaaggcaa tcacctgtgc cccagctgcg aagtcagcgg caattgccag ttgcaggcgc
     1441 tggcctatga tctgggcatg acccactacg aattcgtccc gctggacccg caccgacaga
     1501 acgacggttc ccatcccgag ctgttcatcg agcaggatcg ctgcattttt tgcgaactct
     1561 gcacccgcag cgcgcagcag caggatcaca aaactgtatt tggcattggc ggacgcggcg
     1621 ccgacaccta cctgtttgcc caggccgatt cgagccggct ggcagacacc gccatcaccg
     1681 cacaggatca cgccgcgcat atctgcccgg tgggctgcat cctgcccaag caaggcagct
     1741 accaaattcc catcggtcag cgtgtatatg acgaacaacc cattcacctg cgcggcaatc
     1801 accgcccgga cgaacaggac gccacgccat gagcactgac tcagcacatg cagcgaatgc
     1861 acataaagtg aatgcacata aagtgaaagt cgccacctgt tcactggccg gctgcttcgg
     1921 ctgccacatg tcgtttctcg acatggacga acgcatcatc ccgctgatcg atctgatcca
     1981 gtttgaccgc tcccccctta ccgacatcaa aatcatcggc gactgcgaca tcggtcttat
     2041 cgaaggcggc ctgtgcaacg aggaaaatgt gcacgtggcg caacaattcc gccagcactg
     2101 caaaattctg gtagccgtgg gcgcctgcgc catcaatggc ggcgtgccgg cactccgcaa
     2161 ccgctacacc gcgcaggaat gtctggacga atcctatctg cacggactgg gcgtggtgga
     2221 cgcgcagatt cccaacgatg ccgaactgcc gttgctgctg cccaaagtag tgccattgca
     2281 gtccgccgtg catgtcgatt atttcctgcc gggctgccca ccgcccgccg atgcctttat
     2341 ggaaatcatc aacgccgtac tgcaaaaacg ggaaccccaa ctggattacg cgacgaggcg
     2401 ctttgactga tgagtgaaac ccgcgtcgtc atcgaccccg tcagccgcgt ggaaggccac
     2461 ggcaaggtca ccttgctggt gaacgacgcc ggcatcgtca cccaggcgcg cctgcacatc
     2521 gtggaatttc gcggttttga aaaattcatc cagggccgcc cctattggga actgccgatg
     2581 gtggtgcagc gcctgtgcgg catctgcccg gtcagccatc atctggtggc cgccaaggcc
     2641 atcgaccaga ttgctggcgt taccgcgctg tcagcacccg cgctgaagct gcgccggctg
     2701 atgcatttcg cccaggtgct gcaatcccac gcgctgcatt tttttcatct ggcatcaccg
     2761 gacctgctgt tcggttttca aagtccggtg gaacagcgca atatcgttgc cgtaatcaag
     2821 cagcaaccgc agatcgccat caagggcgtg aaaatccgca agttcggcca gcagattctg
     2881 gaagccattt ccggcaagcg cattcacggc atggcggcga ttcccggcgg catgaacgcg
     2941 ccgctcacac cggaaaaaat ccagccgttc acgcaggatg tgcagcagat catcgactgg
     3001 tgcgaggaga cggttaacct gtcgcgccag ctgaccgacc agaaccgcga ttttcatctg
     3061 cagttcggcc tgatcgaatc gcctttcatg gggctggtgg gaaaaaacgg cgagctggaa
     3121 ctgtacgacg gcaacctgcg ggtgatcgac gccaaaggca acatcctgca ggaccagttt
     3181 ccctgcgatc agtactcgca actgatccag gaacaggtga aatcctggag ctacatgaaa
     3241 ttcccctttc tcacccggct gggcagcgag cagggctggt accgcgtcgg cccgctggcc
     3301 cgcatcaatc tgtgcgattt cattcccact ccgcgcgcgg aacaggcgcg gcagcaattc
     3361 cgccagtacg ccggcgcccc tgcccattcc ccgttgctgt tccactgggc gcgcatgatc
     3421 gag
//
LOCUS       DASUEG010000300         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_90246, whole genome shotgun sequence.
ACCESSION   DASUEG010000300 DASUEG010000000
VERSION     DASUEG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90246"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFX11_05545"
     CDS             <1..578
                     /locus_tag="VFX11_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4938116.1"
                     /translation="SPLRHWKVGPGQKVGVVGLGGLGHMAVKFARAFGAHVVVLTTSE
                     KKKADALMLGAHEAVLSTSRDEMKAHRSSFHFILDTVSAPHDVNALLGLLKRDGTLCQ
                     VGLPAEPLPVGVFHLVGQRRQFAGSSIGGIAETQEMLDFCGEHNIVCDIEKIDIQQIN
                     DAYERLAKSDVKYRFVIDMASLQKDDARTDV"
     gene            571..1464
                     /locus_tag="VFX11_05550"
     CDS             571..1464
                     /locus_tag="VFX11_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX4938117.1"
                     /translation="MYDHLPVLGIGVSLSLSAQPDPVALVQEAGGPRFVEYAGQAEVE
                     RVLPEVERIRAAGVPVLFHPSYINFCGTFANSSGWLAETARHIGAVGSPWFAQDCAYC
                     FWQEGHGYSTQLGYFLPPILNEASLQQAIARVREVKAAVPVTVAIEPPPMTFVVGHMP
                     LFTFFGRLAREADCALLLDMGHLVSWEMASGQRVLDAIDDLPCERVIEVHIAGGKLRE
                     TSEGPVYVDMHESTILDEVWGMLEAMLPLLPNVKALCYECEGMDHDTVMTGLQRLRGL
                     VREHSGSPALVASVVQQEGDA"
     gene            1461..2390
                     /locus_tag="VFX11_05555"
     CDS             1461..2390
                     /locus_tag="VFX11_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938118.1"
                     /translation="MSLVAQEQVLFELLFDRTLRERFVQDGIAALSGRGLSDDERRDF
                     AAVRPEALTLDATMRAGFILSQFCRSYPLSFSLVSSLPQGIELLRQLVDVETMRATPL
                     QRLVTFGSRLKEQVQQQSFASAREQALVISILDAEIGMAWTSAALKQEMLSGNPVPDE
                     VDVPADWLRRSVTIAPFVSAAVIPSSHAGLKTALCAVTGASLWRQLSRQPLSAAERQH
                     VLQKHDPRILVARAVVAQPSRCEPVTEHRTVELSEGFASLLGHVNGSVSIADILQQLG
                     QAGAGEALLGSVQQGFWQLAQQGMLTFSVLRTR"
     gene            2402..2749
                     /locus_tag="VFX11_05560"
     CDS             2402..2749
                     /locus_tag="VFX11_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX4938119.1"
                     /translation="MKRAADEFDEFVRFVIEQMGRDLRPRVRRMFGGYGLYRDDLMFA
                     IIIQDKLYLKTDDVNRREFEEKGLLPFTYESKGKPVTIRYYEAPPEVFDDRDDMQAWA
                     ASAVAAALRARRQ"
     gene            2771..3193
                     /locus_tag="VFX11_05565"
     CDS             2771..3193
                     /locus_tag="VFX11_05565"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX4938120.1"
                     /translation="MDNVNFSPLNELETVLQSVRAGGASVSVFMEALMVAQVFVLLDQ
                     DPGPEGDWHESASPLVLSNHQGMPVLAIFTAPERAIAMTSQFPAFAHGLLIDFTWLLQ
                     RISAGAGLVINPGTLLGMEMPPAGVQRLQQDLRAGGVA"
     gene            3277..>3899
                     /locus_tag="VFX11_05570"
     CDS             3277..>3899
                     /locus_tag="VFX11_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX4938121.1"
                     /translation="MLVMVSGSVLWADGWCRDLSGSRGAGVYQAGWGRQEIAIVPKGY
                     AMQGYGMWHHRARGVQSPLHARALFLQDASGRSLIFCCLDMGYVTHAMRSGLCGVLRE
                     RMGSVFNEDALVLTCTHTHSGPGGCTHDAMYNLVTPGFVPQHVERIVEAATAAILQAW
                     QSAAPADLGLALGAFADGVPVAWNRSVEAYNRNPDVIKRRETETHLAL"
BASE COUNT          679 a          984 c         1346 g          890 t
ORIGIN      
        1 attctccgtt gcgccactgg aaggtggggc cggggcagaa agtgggcgtg gtcgggctcg
       61 gtggcctggg acatatggca gtgaagtttg cacgtgcctt tggtgctcat gtcgtggtgt
      121 tgaccacctc ggaaaaaaag aaagcggatg cgttgatgct gggtgcacat gaagcggtgt
      181 tatccaccag ccgtgacgaa atgaaggcgc atcgcagcag tttccatttc attctggata
      241 cggtgtcggc tccgcatgac gtgaatgctt tgctgggttt gctcaagcgc gatggcacgc
      301 tgtgccaggt gggcttgccg gccgagccgc tgccggtggg tgtgtttcat ctggtggggc
      361 agcgtcgcca gtttgcgggc tcgtcgattg gcggaattgc cgaaacccag gagatgctgg
      421 atttctgcgg cgagcacaac atcgtgtgcg acatcgagaa gatcgatatc cagcagatca
      481 atgacgccta cgagcgtctg gcgaagagcg atgtgaaata ccgctttgtc atcgacatgg
      541 cttccttgca aaaagacgat gcccgcaccg atgtatgacc atctgccggt attgggaatt
      601 ggtgtcagcc tgagcttgtc ggcgcagccc gatccggtag cgctggtgca ggaggcgggt
      661 ggtccgcgct ttgtcgagta tgccgggcag gccgaggtgg agcgtgtgct gccggaggtg
      721 gagcgtattc gtgctgccgg tgtgccggtg ttgtttcatc cttcctatat caatttctgt
      781 ggcacattcg cgaattcttc tgggtggctg gccgaaacgg cacggcatat cggtgccgtg
      841 ggttccccgt ggtttgcgca ggactgtgct tactgcttct ggcaggaggg tcacggctat
      901 tcgacgcagt tgggttactt cctgccgccc atcctgaatg aggcgtcgtt gcagcaggcg
      961 attgctcgcg tgcgcgaggt gaaggctgct gtgccggtta ctgtcgccat cgagccacct
     1021 cccatgacgt ttgtggtagg ccatatgccg ctatttacgt tcttcggacg cttggcgcgt
     1081 gaggcggatt gtgcgctgtt gctggacatg gggcatctgg tgtcatggga aatggcaagc
     1141 ggccagcgtg tgctcgatgc gattgacgac ctgccgtgcg agcgcgtgat cgaagtgcac
     1201 attgccgggg gaaaactgcg tgagacctcg gaagggcctg tgtatgtgga catgcacgag
     1261 agcacgatcc tggatgaggt ctggggcatg ctggaggcca tgttgccatt gctgcccaac
     1321 gtgaaggcgc tttgctacga gtgcgaaggc atggatcacg ataccgtgat gaccggcctg
     1381 cagcgtttgc gtgggctggt acgcgagcac agtggcagtc cggcgctggt ggcaagtgtt
     1441 gtgcagcagg agggcgacgc atgagcctgg tagcgcagga gcaggtgctg ttcgagctgc
     1501 tgtttgatcg cactttgcgt gagcgctttg tgcaggatgg tattgctgcg ttgtccggcc
     1561 gtggcctgag tgatgacgag cgccgtgatt ttgcagccgt tcgtcccgag gcgctgacgc
     1621 tggatgccac catgcgtgcc ggtttcatcc tgtcgcagtt ctgccgcagt tatcctttgt
     1681 ccttcagcct ggtgtcgtcc ctgccgcagg gcatcgagtt gctgcggcaa ctggttgacg
     1741 tggagaccat gcgggcgacg ccgttgcagc gtttggtgac gtttggctcg cgtctgaaag
     1801 agcaggtgca gcaacagtcg tttgcatcgg cccgtgagca ggcactggtc atcagcattc
     1861 tggacgcgga aatcggcatg gcctggacgt ccgctgcatt gaagcaggaa atgttgagcg
     1921 ggaatcccgt accggatgag gttgatgtgc ctgcggattg gctgcggcgg tcggtgacca
     1981 ttgcgccctt tgtgagcgct gcggtcattc cgtcgtctca tgccggattg aagactgcat
     2041 tatgtgcggt aaccggcgct agcctgtggc ggcagctgag ccgtcagccc ttgtcggcag
     2101 ccgagcggca gcatgtattg caaaagcatg atccccgcat tctggtggca cgggccgtag
     2161 tggcgcagcc gtcacgttgt gagccggtga ccgagcatcg cacggtggag ctgtcggaag
     2221 gatttgcctc gttgctggga catgtgaacg gctctgtttc cattgccgac attcttcagc
     2281 agttgggaca ggccggtgcc ggtgaggctt tgctgggcag tgtgcagcag ggcttttggc
     2341 aactggcaca gcagggcatg ctgacgtttt ctgtcctgcg tacacggtaa acggagcgct
     2401 catgaagcgg gcggcagatg agttcgacga gtttgtccgg tttgtgattg agcagatggg
     2461 gcgtgacttg aggcccaggg tccggcgcat gtttggtggt tatggtctgt atcgggatga
     2521 cctgatgttt gccatcatca tccaggacaa gctgtacctg aaaacggatg acgtgaaccg
     2581 gcgggagttt gaggaaaagg ggctgttgcc gttcacgtat gaatcaaaag gcaagcccgt
     2641 cacgatccgc tattacgagg cacctccaga ggtgtttgat gatcgcgatg atatgcaggc
     2701 atgggccgca agcgcagtgg cggccgcgct cagggccaga cgccagtaac tggcaggcaa
     2761 agaggtaaaa gtggataacg tgaatttctc gccattgaat gaactggaga ccgtgctgca
     2821 gtctgtgcgt gcaggcgggg catccgtgtc ggtattcatg gaggcgctga tggtggcaca
     2881 ggtgttcgtg ttgctggatc aggaccccgg acccgaaggg gactggcatg agtcggcctc
     2941 acccttggtg ctgagcaatc atcagggcat gccggtgttg gccattttca cggccccgga
     3001 gcgcgccatt gccatgacga gccagtttcc ggcgtttgcc cacgggttgc tgatcgattt
     3061 tacctggttg ctgcagcgca tcagtgcggg tgccggactg gtgatcaatc cggggacctt
     3121 gctcggtatg gaaatgccgc ctgcgggagt gcagcgcttg cagcaagacc tgcgtgcagg
     3181 gggcgtagct tagtactgcc atcttgagcc gaacggcatg caggatgacc tgcgtggcca
     3241 actcgccggc attcgtttcg cgttacgctg catgccatgt tggtcatggt gagcggttct
     3301 gtcctgtggg cagacggctg gtgccgtgac ctgtccggat caagaggtgc tggtgtgtat
     3361 caggcgggct ggggcaggca ggaaatcgca atcgttccga aaggttatgc catgcagggt
     3421 tacggcatgt ggcatcaccg cgcgcggggt gtgcagagcc cgttgcatgc ccgcgccctt
     3481 tttctgcagg atgcaagcgg ccgcagcctg attttctgtt gcctggatat gggatatgtc
     3541 acccacgcca tgcgcagcgg cctttgtggc gtgctgcgtg agcgcatggg cagtgtgttc
     3601 aatgaggatg cgctggttct gacatgcacg catacacatt ccggcccggg aggttgtacg
     3661 cacgatgcga tgtacaacct cgtgacgcca ggctttgtgc cgcagcatgt ggaaaggatt
     3721 gttgaagcgg cgacggcggc catcctgcag gcgtggcagt ccgctgctcc tgccgatctg
     3781 ggattggcgc tcggggcatt tgccgatggt gtgccggtgg catggaaccg ctcggtggag
     3841 gcctacaatc gcaatcccga cgttataaaa cgtcgcgaga cggaaaccca tctggcact
//
LOCUS       DASUEG010000301         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1358891, whole genome shotgun sequence.
ACCESSION   DASUEG010000301 DASUEG010000000
VERSION     DASUEG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358891"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFX11_05575"
     CDS             <1..584
                     /locus_tag="VFX11_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX4938122.1"
                     /translation="LMDSHGIDLYTEHLSYCSDDGHLYDLMPIPFTEEAVHYVAARIR
                     QVQDILERRIGIENVSYYAAPGQQMREIEFLNAVLDEADCDLLLDVNNIYVNSVNHQY
                     DALEFLRAIPARRIAYLHVAGHFNEAEDLIVDTHGAAVVDPVWQLLDQTYALFGAKPT
                     LLERDFNIPPLPELLGEVEQIRAIQTRHQEVIA"
     gene            581..1354
                     /locus_tag="VFX11_05580"
     CDS             581..1354
                     /locus_tag="VFX11_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEX4938123.1"
                     /translation="MNSTTATERLQRAFAAHIRDPRSHPAPAGIEDRRMAIYRELFHN
                     NITSFLAGGFPVLCKVLSAERWQAITHDFFARHRSTTPYFLQIGQEFLQYLQQEYQPH
                     PDDPPFLLELAHYEWLELAADVDPDEIPVAGFNPRGNLLDGRPLLSPLAYVACYEFPV
                     HRISPDFQPREPGGHPVWLIIYRDIEDRVRFMEINAVTARLLMLLQDDGSLTGRAAIE
                     LVQAELQHPQPEVVLQGGLQALEHLRQTGIVLGTELNTA"
     gene            1410..2057
                     /locus_tag="VFX11_05585"
     CDS             1410..2057
                     /locus_tag="VFX11_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007621305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX4938124.1"
                     /translation="MNLLSTLARRLHTLLEKFRAADFLAPLAFRLYLAPIFISVGLHK
                     ALHFDDIVQWFQFGLELPAPYLMALLATAAELGGGLALLLGVATRWMAVPLMVTMIVA
                     AGTAHWHNGWFAVAPSDPDTSIASLLAPIGFPGAQESLANSGEVAQRLERARELLREH
                     GNYDWLTETGNLVVLNNGIEFAFTYFILLLSLFFTGGGRYVSIDYWLARRMSLVR"
     gene            2157..2918
                     /locus_tag="VFX11_05590"
     CDS             2157..2918
                     /locus_tag="VFX11_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX4938125.1"
                     /translation="MKKLLSGILLAVAGWTVLPAEASQEADPWEGFNRVMFTFNDTLD
                     RYLLKPVAIGYDKVMPNPLQAGVSNFFNNLGEVKTITNDLLQAKFGQAGLDSTRLLVN
                     TTVGLGGFIDIGSRIGLEQNDEDFGQTLGYWGIGSGPYLVLPLLGSSTVRDAVGRYPD
                     YLVHPTNLVEHDRTRYSLDALYVIDVRASLLEVEKLVAADRYTFFREAYLQRRQFQVQ
                     DGVMEDDFEGEEDLFEDEESGDIPDEGAGDAGFEE"
     gene            3031..>4051
                     /locus_tag="VFX11_05595"
     CDS             3031..>4051
                     /locus_tag="VFX11_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938126.1"
                     /translation="MKQFSCRAAAVAFALAASAGFATTPEVLNRGWKPSIEIAGSGDT
                     ATAVNNMRAMADLQRVVLQNTEFHQQTQQFVDELWASLESFNLNRYYELLPETDGDWR
                     QLRVYGARDGKLYRVGPDADKIGIDADGKVREMGDMAMQVRAKRLASSGETHYLLQNS
                     MELGVGQVAWPSVKQATEETLRIVVDGSPEFSSKAERSAAQQYRDKVIRMNSSLGSED
                     VDVIAPLWASFPAMWDLLAQMGRIEDVVYHDLSQGYRKMNGSFVIDPELMKRNYPALA
                     SHVLKMDRLFSGGIRLTDARGELLTAEIDSQTLRGQFQAFVADGRIVPVSGGRVLLDA
                     PPIPDG"
BASE COUNT          797 a         1171 c         1204 g          879 t
ORIGIN      
        1 gattgatgga ctctcatggc attgatctgt acaccgagca tctcagttat tgcagcgacg
       61 atggccatct gtacgacctc atgccgattc catttaccga agaagccgtg cattacgtcg
      121 ccgcgcgcat ccgccaggtg caggatattc tggaacgtcg tattggcatc gaaaacgtgt
      181 cgtactatgc agcgcccggc cagcagatgc gggagatcga gtttctcaac gctgtgctcg
      241 acgaagccga ctgcgatcta ctgctggatg tgaacaacat ttacgtcaac agcgtcaatc
      301 atcaatacga cgcgctggaa tttctgcgcg cgattccggc ccggcgcatc gcctatctgc
      361 atgtggccgg tcatttcaat gaagcagaag atctgatcgt cgacacccac ggcgcagcgg
      421 tggtcgatcc ggtctggcaa ttgctggatc agacttacgc gctgtttggc gcgaagccca
      481 ccctgctgga gcgggatttc aatattcctc ctctgccgga actgctgggg gaggtggaac
      541 agattcgcgc gattcagacc cgccatcagg aggtgatcgc atgaacagca cgaccgcaac
      601 cgaacggctg caacgtgcct tcgctgccca tatccgcgat ccgcgcagtc atccggcgcc
      661 tgccggcatc gaagaccgtc gcatggccat ctaccgcgaa ctgttccaca acaatatcac
      721 cagctttctg gcgggtggtt ttcccgtgct ctgcaaggtg ctcagtgccg agcgctggca
      781 ggcgatcacg catgattttt ttgcccgtca tcgcagcacg acgccctatt tcctgcagat
      841 cggccaggaa tttctgcagt acctgcagca ggaataccag ccacacccgg atgatccgcc
      901 ttttctgctg gaacttgcgc actatgaatg gctggagctg gcagcggacg tggatccaga
      961 tgagattccg gtcgctggct tcaatccccg gggcaatctg ctggatggtc gtccgctgct
     1021 gtcgccgctg gcctatgttg cctgctatga atttcccgtg catcgcatca gcccggattt
     1081 tcagccgcgg gaaccgggcg ggcatccggt ctggctcatc atttaccgtg atattgaaga
     1141 ccgtgtgcgc ttcatggaaa tcaacgcggt gactgctcgc ctgctgatgc tgttgcagga
     1201 cgatggctcg ctgaccggtc gagccgccat cgaactggtg caggcagaat tgcagcaccc
     1261 gcagcccgag gtggtcctgc agggtggact gcaggctctg gaacacctgc gccagaccgg
     1321 cattgtgctg ggcacggagc tcaacaccgc ttaagccgct gtttgatcga atcctttcct
     1381 ttcagatcca ttaatggagg tcatccccaa tgaacctgct ctccacgctt gcccgtcgtt
     1441 tgcatacact gctggaaaaa ttcagggcgg ccgattttct cgcgccgttg gcattccgtc
     1501 tttatctggc gccgattttc atctcggtgg gattgcacaa ggcgctgcac tttgacgaca
     1561 tcgtgcagtg gtttcaattc ggactggagc tgcccgcgcc gtatctgatg gcactgctgg
     1621 caacagcggc cgaacttggc ggaggactcg ctttgctgtt gggcgtcgcc acccgctgga
     1681 tggcggtgcc actgatggtg accatgatcg tcgctgcggg cacggcccac tggcacaacg
     1741 gctggtttgc ggtcgcgccg tccgatccag atacgagcat cgccagtctg ctggctccga
     1801 tcggttttcc tggcgcgcag gaaagccttg caaacagtgg ggaggttgcg cagcgactgg
     1861 agcgtgcgcg ggagttgctg cgcgaacacg gcaattacga ctggctgacg gaaaccggta
     1921 atctcgtggt gctcaacaac ggtatcgaat tcgctttcac ctatttcatc ctgctgctga
     1981 gcctgttctt caccggtggc ggccgatacg tcagcatcga ttactggctg gcccggcgca
     2041 tgtcgctggt ccgataatgc gagcggctcc actttttgtg tcgggtgagg ttttgtattg
     2101 tccagtccaa aacgatagaa tcccgagcgt tcgagtgtga ggggcgaagg atcatcatga
     2161 aaaagctgtt gtccgggata ttgctggccg ttgcgggatg gacggtgctg cctgccgaag
     2221 ccagtcagga ggctgatccc tgggaaggct tcaaccgggt catgtttacc ttcaatgaca
     2281 cgctggaccg ttatctgctc aagcctgtgg ccatcggcta tgacaaggtc atgcccaatc
     2341 cgctgcaggc gggtgtgagc aacttcttca acaacctcgg tgaagtcaaa accatcacca
     2401 atgatctgct gcaggcgaag tttggccagg cgggtctgga cagcacccgc ttgctggtga
     2461 acaccacggt cggacttggc ggctttatcg acatcggatc gcgcatcgga ctggaacaga
     2521 acgacgagga tttcggccag accctcggtt attggggcat tggttccggt ccctacctcg
     2581 tcctgccgct gctgggctcc agcaccgtgc gcgatgccgt cggacgctat ccggattacc
     2641 tggtccaccc caccaatctg gtggagcacg atcgcacccg ctactccctg gacgctctgt
     2701 acgtgatcga cgtgcgcgcc agcctgctcg aggtggaaaa gctggtggcg gcagaccgtt
     2761 acaccttctt ccgcgaagcc tacttgcagc ggcggcaatt ccaggtgcag gacggtgtca
     2821 tggaggacga ttttgaggga gaagaagacc tgttcgagga tgaggaatca ggcgacatcc
     2881 cggacgaagg ggccggagac gccgggttcg aagagtaaga tcaggccaat acagcaagaa
     2941 atttgtgcca tttcccgtac aattgccgct ggggtatact ccgtgtgtct ctctttccag
     3001 cgccagtgat cataataaaa aggaatggcc atgaaacagt tctcttgccg ggcagcggct
     3061 gtcgcctttg cccttgccgc atcagccggt tttgccacca cccccgaagt tctcaaccgg
     3121 ggttggaaac cttccattga aattgccggc tccggcgaca ccgccaccgc agtgaacaac
     3181 atgcgtgcca tggccgatct gcagcgggtc gtgctgcaaa acaccgaatt ccaccagcag
     3241 acccagcaat tcgtcgatga actctgggcg tcgctcgaat ccttcaacct taatcgctac
     3301 tacgaactgc tgccggaaac cgatggcgac tggcgtcagc tgcgggtcta tggtgcccgt
     3361 gacggcaagc tctatcgcgt aggcccggac gcagacaaga tcggcattga tgccgatggc
     3421 aaggtccgtg aaatgggtga catggccatg caggtgcgtg ccaagcggct ggcttcttca
     3481 ggcgaaaccc actacctgct gcagaactcg atggagcttg gggtagggca ggtggcctgg
     3541 ccttccgtca aacaggccac cgaggaaacc ctgcgcattg tcgtggatgg ctcgccggaa
     3601 ttcagcagca aggcggaacg cagcgctgcc cagcagtacc gtgacaaagt gatccgcatg
     3661 aactcgtcgc tcggctctga agatgtcgat gtgatcgcac ccttgtgggc ctcgtttcct
     3721 gccatgtggg acttgctggc gcagatgggt cggatcgagg acgtggtcta tcacgatctg
     3781 agccagggtt accgcaagat gaatggctcc ttcgtcatcg atcctgaact gatgaaacgc
     3841 aattaccccg cgctggccag ccacgtgctc aaaatggatc ggctgttttc gggaggaatc
     3901 cgccttacgg atgcgcgcgg tgaattgctg acggcggaga tcgacagcca gacattgcgg
     3961 ggccagttcc aggcatttgt cgccgatggc cgtatcgtgc cagtgtcggg cggacgggtg
     4021 ctgctggatg cgccgccgat tccggatggc c
//
LOCUS       DASUEG010000302         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1056503, whole genome shotgun sequence.
ACCESSION   DASUEG010000302 DASUEG010000000
VERSION     DASUEG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056503"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..496
                     /locus_tag="VFX11_05600"
     CDS             41..496
                     /locus_tag="VFX11_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4938127.1"
                     /translation="MMTKLERSLLDRYQRGLPISATPWADMAQELGVSEQDVLSTLEK
                     LRDQKFITRVGPVFDHHRAGASTLVALAVPPERLEEIADFINQFDAVNHNYAREHEFN
                     LWFVVTASDQARLQSVLDTIECATGMPMLNLPMEHAYHIDLGFKLAWQE"
     gene            501..989
                     /locus_tag="VFX11_05605"
     CDS             501..989
                     /locus_tag="VFX11_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HEX4938128.1"
                     /translation="MTLTASQQETLRAAIQDGLPLTARPYATLAQQIHASENDVIGQI
                     QHWLDDGLIKRMGIVVRHHAIGYSANAMVVWNVPDELVDFVAEKMRVFPSVTLCYRRP
                     RRLPDWPYNLFCMIHGRDRHWVLQQIEQLVQHAELQSIPRAVLFSQRQFKQTGGRYAR
                     VG"
     gene            1009..1455
                     /locus_tag="VFX11_05610"
     CDS             1009..1455
                     /locus_tag="VFX11_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX4938129.1"
                     /translation="MLDALDRQLLNRLQEGFPVCDRPYLAVAREFSIDEGELIARIQR
                     LLDTNVLTRFGPMYHAERMGGGLSLAAMAVPAEDFERVALLVNSLPEIAHNYARDHDF
                     NMWFVIATEDRDDVAAVVKRIQALTGYTVYNMPKIEEFYVGLRFAV"
     gene            1430..1948
                     /locus_tag="VFX11_05615"
     CDS             1430..1948
                     /locus_tag="VFX11_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX4938130.1"
                     /translation="MSACDLLCDNVMQLAECDRQLIELTQAGLPLVPRPYHELARQMG
                     ISADEVMLRIQRMQDNGVIRRIAAVPNHYKLGFRANGMTVWNVPDEKVSVLGEQVGAL
                     DFVSHCYHRPRHLPQWPYNLFAMVHGASRDDVQDHIAAIARLLGDDNHGSDVLFSTRI
                     LKKTGLRIRTRE"
     gene            1959..>2105
                     /locus_tag="VFX11_05620"
     CDS             1959..>2105
                     /locus_tag="VFX11_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HEX4938131.1"
                     /translation="MFRITQYMQTLLNPEPVGPARKPPGPVVIWNLIRRCNLTCKHCY
                     SISAD"
BASE COUNT          433 a          641 c          599 g          432 t
ORIGIN      
        1 gcgggatttt cttcaccaac cgcgcccacg agatcggcct atgatgacca agcttgaacg
       61 cagtctgctc gaccgttacc agcgtggcct gcccatcagt gccacaccct gggccgacat
      121 ggcgcaggaa ctgggcgtga gcgaacagga cgtcctgtcc acgctggaaa aattgcgcga
      181 ccagaaattc ataacccggg tcggccccgt gttcgatcat catcgcgccg gcgccagtac
      241 gctggtggca ttggcggtgc cgccagagcg gctggaggaa atcgccgatt tcatcaacca
      301 gttcgacgcg gtcaatcata attacgcccg cgagcatgaa ttcaatttgt ggttcgtcgt
      361 caccgccagc gatcaggcgc ggctgcagtc agtgctggat acgattgaat gcgccaccgg
      421 catgcccatg ctgaatttgc cgatggagca tgcctatcac atcgatctgg gtttcaaact
      481 ggcgtggcag gagtgaccgc atgacactga cagcatcgca gcaggaaacc ctcagggccg
      541 ccatccagga tggcctgccg ctcactgcac gcccctatgc gacactggcc caacagatcc
      601 acgccagtga aaatgacgtg atcgggcaga tacaacactg gctggacgat ggcctgatca
      661 aacgcatggg aattgtggtg cgtcaccacg ccatcggcta cagcgccaac gccatggtgg
      721 tgtggaatgt accggatgaa ttggttgatt tcgtggcgga aaaaatgcgc gtgtttcctt
      781 cggtgacact ttgctaccgg cgtccccgcc gtttgccaga ctggccctac aacctgtttt
      841 gcatgattca tggccgggac cggcattggg tcttgcagca gatcgaacaa ttggtgcagc
      901 atgcggaatt gcagtccatt ccacgcgccg tattgttcag tcagcgccag ttcaagcaaa
      961 ccggaggccg ctatgcgcgg gtcggctgaa acacaacaaa cggaattggt gctagacgcc
     1021 ctcgatcgcc agctgctcaa ccggctgcag gagggttttc ctgtctgtga tcgtccctac
     1081 ctggcggttg cccgtgaatt ttccatcgac gaaggcgaac tgatcgcccg catccaacgt
     1141 ctgctcgaca ccaatgtcct cacccgtttt ggtcccatgt atcacgccga gcgcatgggc
     1201 ggcggtttgt cgctggccgc gatggcagta ccggcggagg atttcgaacg tgttgccttg
     1261 ctggtgaaca gcctgccgga aatcgcccac aattatgccc gcgaccatga tttcaacatg
     1321 tggttcgtga tcgccaccga agaccgcgat gatgttgccg ccgtggtcaa aagaattcag
     1381 gcgctcaccg gctacacggt ttacaacatg cccaagatcg aggaatttta tgtcggcctg
     1441 cgatttgctg tgtgacaacg tgatgcagct ggccgaatgc gatcgccagc tgattgaact
     1501 gacccaggcc ggattgccgc tggtgccacg gccttaccat gagctggccc ggcagatggg
     1561 catttccgcc gacgaagtca tgttgcgcat ccagcgcatg caggacaacg gcgtgatccg
     1621 tcgcatcgcc gccgtgccca atcactacaa gctgggcttt cgcgccaacg gcatgaccgt
     1681 gtggaatgtg ccggatgaaa aagtcagtgt tcttggcgag caggttggtg cactggattt
     1741 cgtcagtcac tgctatcacc ggccgcgtca tctgccccag tggccctata atctgttcgc
     1801 catggtgcac ggcgccagtc gtgacgacgt gcaggaccac atcgctgcca tcgcccgtct
     1861 gctgggtgac gacaatcacg gcagcgacgt cctgttttcc acccgcatcc tgaaaaaaac
     1921 cggcctgcgg attcgcacgc gggaataagc gaggttccat gttccgcatc acccagtaca
     1981 tgcagacgct gctgaatccc gagccggtag gccccgcccg caagccgccg ggcccggtgg
     2041 tgatctggaa cctcatccgc cgctgcaacc ttacctgcaa gcactgctat tccatttccg
     2101 ccgac
//
LOCUS       DASUEG010000303         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_774968, whole genome shotgun sequence.
ACCESSION   DASUEG010000303 DASUEG010000000
VERSION     DASUEG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774968"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VFX11_05625"
     CDS             complement(<1..1212)
                     /locus_tag="VFX11_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4938132.1"
                     /translation="MKPNFKILCVLSLLLVAVAIALSPNILNIRYVNDITEYFSPEDP
                     RLIAFKKVEVDMGAQESLLVLLQLKNQSFLSATGTATLYDTVRKIEALPGILRVNSLL
                     SHAVSTPTSDTLSAYRLLSKESHASDAVLGQLADASTHNRAVLSKDKSISAIYVYFTD
                     SSAIEQNYPAIEAILAEQASLHQLQATYLLGSVEIKHALNQALLHDCVYLMPLVLMSG
                     LLLLYFFLRSWWLVFSGILSIVVALWITAGIAGTLGFTINQTSGLAFAIAFIVSLADI
                     IHLLMSYSRHPLINNSNVETMMESMRGNVVALFLTSITIAIGFISLGVGDSPVFTTFG
                     YIATIGVICSFITAVTITPVVAVRISPPNNAREPDIFQRLVRQIWRVARNMRGPRAWA
                     FYGISFLLSAGM"
     gene            1345..2118
                     /locus_tag="VFX11_05630"
     CDS             1345..2118
                     /locus_tag="VFX11_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4938133.1"
                     /translation="MLFGKNKSLVCFSPNPRAAVRLFCFPCAGGGASMYRRWATLLPG
                     VDVWAVNYPGRETLHGQPFAPSLDAVLAPVLEQQELFAEKPFLVYGHSFGSLVAFAAA
                     VRLQALGCQTRGVLGSARRAPHLPTGQVISDLPDDRFLAELDRFGGMPDAIRKDPEML
                     AFYLPIIRADLHLNDISRLPDTDRIDAPVYVFSGSHDRVATQAELLAWKNSSTGRVEH
                     REFTGGHFFIQDKAEEFTACIGNVIGTIMQEPDEDLVAF"
     gene            2439..>4054
                     /locus_tag="VFX11_05635"
     CDS             2439..>4054
                     /locus_tag="VFX11_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX4938134.1"
                     /translation="MAATRAQLKGEQELTAFLGDDGSREGTLTFAGLDRAARNIAAGL
                     TAKGLARKNLMLLYTPGIDYIKAFFGCLYAGSVPVPAYPPMGARDIDRLKRVVLDCDA
                     AAILSSSMLLPMIEAWVANPTNGLNIKCVPTDLAANEDAIGFRPADVDPSEVAFLQYT
                     SGSTGHPKGVMVSHHNLLENFKQIVWTFAHSADLDAIAPEYKTVIWLPPFHDMGLIGG
                     VLTPVFVGTRVTLMSPLTFLKNPFVWLKTITDEKAKVSGGPNFSYQYCVRKVSDEQMA
                     QLDLSSWQVAFNGAEPILMDALHAFTDKFRSAGFDPRAFLPCYGLAEATLFVAGSPAM
                     RGARVIEADLQQLARGEVNPAEPATSADTATLVSSGVIAEGAEVRIVDPNTHVACHDG
                     KVGEIWVNSPAVAQGYWNKPSFSDSVFRAHIKGDDDKTWMRTGDMGMMWDNELFVTGR
                     IKELIIVAGRNHYPQDIEVSLQASNPLFRKGCGAAFSVVDHGKEQLVVMQEVSGNGMQ
                     PQDFHQLALQGARAIATRHGISPKALLFIQA"
BASE COUNT          839 a         1109 c         1237 g          869 t
ORIGIN      
        1 catgccggcg gacagcagga aactgatgcc gtaaaaggcc caggcccgcg gcccgcgcat
       61 gttgcgcgcc acccgccaga tctggcggac cagacgctgg aaaatgtccg gctcgcgtgc
      121 gttgttgggc ggcgatatcc gtaccgcaac gactggcgtg atggtgacag ccgtgatgaa
      181 ggagcagatc acaccgatgg tggcgatgta gccgaaggtg gtgaacaccg gcgaatcgcc
      241 cacgcccagg ctgatgaagc cgatggcgat ggtgatgctg gtgagaaaca gcgccaccac
      301 gttgccacgc atggattcca tcatggtttc cacattgctg ttgttgatca gcggatgccg
      361 cgaataactc atcagcaggt ggatgatatc ggccagcgac acgatgaagg caatggcaaa
      421 agccagtcct gaggtctgat tgatggtgaa acccagtgtg ccggcgatgc cggcggtgat
      481 ccacagtgcc accacgatgg acaggatgcc ggagaacacc agccaccagg aacgcagaaa
      541 gaaatacagc agcaacaggc cgctcatcag caccagcggc atcaggtaca cgcagtcgtg
      601 cagcagtgcc tggttcagcg cgtgtttgat ttccaccgat cccagcagat aggtagcctg
      661 cagctgatgc agcgatgcct gctccgccag gatcgcttcg atcgcaggat aattctgttc
      721 gatggcgctg ctgtcggtga agtagacata gattgcggag atgctcttgt ctttcgacag
      781 caccgcgcga ttgtgggtgg aggcatcggc cagctggccc agcacggcgt cgctggcgtg
      841 ggattccttg ctgagtaacc gataggctga caaggtgtcg gaggtgggtg tgcttaccgc
      901 gtggctgagc agcgaattta cccgcagtat gcccggcagc gcctcgatct ttctgacggt
      961 gtcatacagg gttgccgtgc cggtggcgct caggaacgac tggttcttca gttgcagaag
     1021 caccagcagg ctctcctggg cacccatgtc cacttccacc tttttgaagg cgatcagacg
     1081 ggggtcttcc ggcgagaaat actcggtaat gtcgttgacg taacgaatgt tgaggatgtt
     1141 gggggacagg gcgattgcca cggccaccag cagcagtgac agcacacaca gaattttgaa
     1201 attcggcttc atggttttgt ttttgttgtg agcgtggccg gggtaacatt cagcggcatg
     1261 ggaggccggc agggaaagaa cggctccgga aaatgtcggc ccgataattc aggcgagtct
     1321 aatcacccgg tgcagaggtc tgcaatgtta tttggtaaaa ataagtcact ggtttgtttc
     1381 agccccaatc cgcgcgcggc ggtacggctg ttctgctttc cctgtgccgg cggcggcgct
     1441 tccatgtacc ggcgctgggc tacgttgctg cccggcgtgg atgtctgggc cgtgaactac
     1501 cccgggcggg aaacgctgca cggccagccg tttgcgccca gtctcgatgc ggtgcttgcc
     1561 cccgtgctgg agcagcagga actgtttgcc gagaaaccct tccttgtgta tggccacagc
     1621 ttcggttcgc tggtggcctt tgcagcggcg gttcggctgc aggcactcgg ctgtcagacc
     1681 cggggcgtac tgggcagtgc gcgtcgggca ccgcatttgc caacgggcca ggtcatttcc
     1741 gatcttcccg acgatcgctt tctggcggag ctggaccggt ttggcggcat gccggatgcc
     1801 atccgcaagg acccggagat gctggccttc tatctgccga tcattcgtgc cgatctgcat
     1861 ttgaatgaca tcagccgctt gccggacacg gatcggattg atgcgccggt gtatgttttt
     1921 tcgggcagcc atgatcgcgt ggccacgcag gcggaactgc tggcatggaa aaacagcagc
     1981 actggccgcg ttgagcaccg cgagtttact ggcggacatt tcttcattca ggacaaggcg
     2041 gaggagttca cagcctgtat agggaacgta attggtacga tcatgcagga accagatgag
     2101 gatctcgtcg cgttctaaac ataattgcgc attctgatta cgaactgtca ccgaattggc
     2161 cgaatcttac aagttttgcg agaactcgcg tcagtacact ggcccttgcc aatgggtcgt
     2221 gctgataaga acaataaagg tagttataac aatacaaagt cgacaaccgc ccaggaattg
     2281 ctggtttgtc agggatttaa cgcacgttgt cgtttgttca tttgtattgc gcactcctat
     2341 ttatacgtga ttagcacacc tgagtcttga atacagggta aagacactag gtggaggtcg
     2401 gtatgaaagg gttcccgcaa tttaagaacc tggtagaaat ggctgcgaca cgcgcacagc
     2461 tcaagggtga acaggagctg acggcgtttc tcggtgatga tggcagccgt gaaggcacac
     2521 tgactttcgc aggtcttgat cgtgctgcac gcaatatcgc tgcggggctg acggccaaag
     2581 gtctggcccg caaaaatctg atgctgctgt acacgccggg catcgattac atcaaggctt
     2641 tcttcggttg tctgtacgcc ggttccgtgc cggtgccggc ttaccctccc atgggtgccc
     2701 gcgacatcga ccgcctcaag cgggtggtgc tggattgcga tgctgccgcg attctctcct
     2761 ccagcatgct gctgcccatg atcgaagcct gggtggccaa tcccaccaac ggcctcaata
     2821 tcaagtgtgt gcccaccgat ctggctgcca atgaagatgc catcgggttc aggcctgccg
     2881 atgttgatcc cagtgaagtg gcgttcctgc aatacacctc cggttccacc ggccacccga
     2941 aaggcgtgat ggtgagccac cacaacctgc tggaaaattt caagcagatt gtctggacct
     3001 tcgcccattc tgcagatctg gatgcgattg cgccggaata caaaacagtc atctggttgc
     3061 caccatttca tgatatgggc ctgatcggcg gtgtgctgac accggtcttt gtgggtacgc
     3121 gcgtcacctt gatgtcgccg cttactttcc tgaaaaaccc gttcgtgtgg ctgaaaacca
     3181 tcaccgatga aaaagccaag gtatcgggcg gccctaactt ctcctatcaa tactgtgtgc
     3241 gcaaggtcag cgacgagcag atggcgcagc tggatctgtc cagctggcag gtggcgttca
     3301 acggagccga gccgattctg atggacgcgc tgcatgcgtt cactgacaaa ttccgttccg
     3361 ccggttttga tccacgcgca ttcctgccct gctatggtct ggcggaagcg acgctgttcg
     3421 tggctggttc gcctgccatg cgcggcgccc gggtgatcga ggcggatctg cagcagctgg
     3481 ccaggggcga ggtgaatccg gcagaaccgg ccacctcggc tgacacagcg acgctggtca
     3541 gttccggtgt gatcgccgaa ggtgccgaag tgcgtatcgt cgatcccaac acccatgtgg
     3601 cctgccatga tggcaaggtg ggcgaaatct gggtgaacag tccggcggtg gcgcagggtt
     3661 actggaacaa gcccagcttc tccgacagcg tattccgcgc ccacatcaag ggtgatgacg
     3721 acaagacctg gatgcgcacc ggcgacatgg gcatgatgtg ggataacgaa ctgttcgtca
     3781 ccggccgcat caaggaactg atcattgttg cgggccgcaa ccattatccg caggacatcg
     3841 aagtgtcgct gcaggcatcc aatcccctgt tccgcaaagg ctgtggtgct gcgttttcgg
     3901 tagtggatca cggcaaggaa caactggttg tcatgcagga agtgagtggc aacggcatgc
     3961 agccgcagga tttccatcag ctggcactgc aaggcgcccg cgcaattgcc acccgccacg
     4021 gcatttcgcc gaaagcactg ttgtttatcc aggc
//
LOCUS       DASUEG010000304         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1106232, whole genome shotgun sequence.
ACCESSION   DASUEG010000304 DASUEG010000000
VERSION     DASUEG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106232"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFX11_05640"
     CDS             complement(<1..762)
                     /locus_tag="VFX11_05640"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX4938135.1"
                     /translation="MSEFWSPVVHSLKPYVPGEQPKLSNLIKLNTNENPYGPSPKVLQ
                     ALQAEVADSLRLYPDPTGADLKAAVARRHGLTPQQVFVGNGSDEVLAHTFQALLKHDQ
                     PLLFPDISYSFYPVYCQLYQIAFQTIPLREDFSIALEDYARPNGGIIFPNPNAPTGKL
                     LALDAIEQLLQAHPRSVVVVDEAYVDFGGQSAIPLVDRYPNLLVVQTFSKSRSLAGLR
                     VGFAVGQAKLIEGLERVKDSFNSYPLDRFALKGAVA"
     gene            889..1341
                     /locus_tag="VFX11_05645"
     CDS             889..1341
                     /locus_tag="VFX11_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938136.1"
                     /translation="MTVATTHSLIDLVSQFHRQLAQHCSQTARHNQDERVRLLLDYVG
                     RHESHLAEALQSYENQAREKILATWFRNAPTDVLPRLDQHALNATSTVEDVINWAVEM
                     DNAIISFYRTLAAEAETPDLKKVLDNLLAMEEREQHRMVRSALRIQDF"
     gene            complement(1400..2359)
                     /locus_tag="VFX11_05650"
     CDS             complement(1400..2359)
                     /locus_tag="VFX11_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX4938137.1"
                     /translation="MDLLLILTYTALCIAIFKIFRIPLNKWSVPTAVLGGVVLIGALI
                     FAMNYNHPFSEISRTYFITTPIVAPVKGILTEVPVEGNQLLEKGDVLFRIDPVPYQNK
                     VASLKAQLKSAKLDLDRARQLVARGALAQRDLDLAQGQVDRLIPDLANAEYDLTRTTV
                     TAPSKGYVIQLFARPGLMAVPMPLRPLMVFVPQESHFIVAWFRQNSLLRLVPGDDAEV
                     TYDGLPGEVFSGKVRQVMPVMGEGQLQSGGNLLSLQGPSVPGRVPVVIDITDPRFEQY
                     AQKMPGGSFGQAAVYSKHFHHVAIMRKILLRMASWMNYLFPFH"
     gene            complement(2359..2739)
                     /locus_tag="VFX11_05655"
     CDS             complement(2359..2739)
                     /locus_tag="VFX11_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEX4938138.1"
                     /translation="MLEYVALGILIFAAITLFYGIIVIHDIPYDIAKHRNHPHQDAIH
                     VAGWVSLLTLHAIWPFLWIWATLYRADRGWGFGGADGASGKDELMNELAGLRARIDAL
                     EQSRTAAVAAPAAVTSVAQEGESQ"
     gene            2864..3325
                     /locus_tag="VFX11_05660"
     CDS             2864..3325
                     /locus_tag="VFX11_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009724942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX4938139.1"
                     /translation="MKQLLLVAHAPSPNTGAMVQAVLRGAGHPDISGVQVRHIPPLQA
                     QPDDVLQADALILGTTENLGYMSGALKDFFDRIYYPCLEQTQGKPFAFYIRAGHDGTG
                     TRRAIESITTGLRWRWVQEPLLCRGPWQDSFLEQCEQLGMTMAASLEAGII"
     gene            complement(3347..>3544)
                     /locus_tag="VFX11_05665"
     CDS             complement(3347..>3544)
                     /locus_tag="VFX11_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4938140.1"
                     /translation="GQIMAQPAFTVNENDLIALLVPLLSDKGLHHVPVVNDERRLVGI
                     VTQSDLIAALFVGAVEMQEAA"
BASE COUNT          748 a         1152 c         1019 g          625 t
ORIGIN      
        1 cgccaccgcg cccttcaacg cgaaacggtc cagcggatag gaattgaaac tgtccttcac
       61 ccgctccagg ccttcgatca gtttggcctg acccacggca aaacccaccc gcagtccggc
      121 cagggaacgg gatttggaaa aggtctgcac caccagcagg ttcgggtaac ggtccaccag
      181 cggaatcgca ctctggccac cgaaatccac ataggcctca tccaccacca ccaccgagcg
      241 cgggtgcgcc tgcagcagct gttcgatcgc atccagcgcc agcagtttgc cggtgggcgc
      301 gttcggattg gggaaaatga tgccaccatt gggccgggca taatcctcca gcgcgatgga
      361 aaaatcctcc cgcagtggaa tggtctggaa tgcgatctgg tacagctggc aatagaccgg
      421 atagaagctg taggaaatgt cgggaaacag cagcggctgg tcatgcttca gcagcgcctg
      481 gaacgtgtgg gccagcactt cgtccgaccc gttgccgacg aatacctgct gcggggtcag
      541 gccgtgccgc cgtgccaccg cggctttcag gtcggcgccg gtggggtccg ggtacagacg
      601 cagggaatcg gccacttcgg cctgcagcgc ctgcagtact ttgggcgacg ggccataggg
      661 gttttcattg gtattcagct tgatcaggtt ggacagcttc ggctgctcgc ccggcacata
      721 gggtttcagg ctgtgcacca ccgggctcca gaattcactc acggcaacaa tctcgcaggg
      781 caaatgtggc ttggaaagct ggccagcata ccatcccgtg acgcagctca acaccctggg
      841 ccccgggaac cactagcttt ccgactataa gccgcaggag tccccgtcat gaccgttgcc
      901 accacccaca gcctcatcga cctggtcagc cagttccacc gccagctggc gcaacactgc
      961 agccagaccg cccgccacaa ccaggacgaa cgggtccggc tgttgctgga ttacgtcggc
     1021 cgccacgaaa gccatctggc ggaagcactg caatcctacg aaaaccaggc ccgcgaaaag
     1081 atcctcgcca cctggtttcg caatgcgccc accgacgtcc tgccgcgcct cgaccagcat
     1141 gccctcaacg ccaccagcac cgtggaggat gtgatcaatt gggctgtgga gatggacaat
     1201 gccatcatca gtttctatcg caccctggca gcagaggcgg aaacccccga cctgaaaaag
     1261 gtgctggaca atctcctggc catggaagag cgcgaacaac accgcatggt tcgttcagct
     1321 ctgcggattc aggatttctg aagcagaaaa ggaagggggg aaccgccccg gctggagcgg
     1381 ttcccgcaga acgccgtgtt cagtggaacg ggaacaggta gttcatccag gacgccatcc
     1441 gcagcaggat cttgcgcatg atggccacat gatggaagtg cttgctgtac accgccgcct
     1501 ggccaaagga gccacccggc atcttctggg cgtactgttc gaagcgtgga tcggtaatgt
     1561 cgatcaccac cggcacccgg cccggcaccg atggcccctg cagcgagaga agattgccgc
     1621 cggactgcaa ctgcccttcc cccatcaccg gcatgacctg ccgcactttt ccgctgaaga
     1681 cctcgccagg caatccatca taggtcacct cggcatcatc gccaggcacc agtcgcagca
     1741 ggctgttctg ccggaaccag gccacaatga aatgggattc ctgcggcacg aacaccatca
     1801 ggggccgcag cggcatcggc accgccatca agcccggacg ggcgaacagc tggatcacat
     1861 agcccttcga cggagccgtc accgtggtgc gggtcagatc atattcggcg ttggccagat
     1921 ccggtatcag ccggtccacc tggccctggg ccagatccag gtcccgctgg gccagggcac
     1981 cgcgcgccac cagctggcgg gcacggtcca gatccagctt cgctgacttc agctgcgcct
     2041 ttaacgacgc caccttgttc tggtaaggca ccggatcgat gcgaaacagc acgtcgcctt
     2101 tttccagcag ctggttacct tccaccggca cctcagtcaa gatgcccttc accggcgcca
     2161 cgatcggtgt ggtaatgaaa taggtacgac tgatttccga aaacggatgg ttgtaattca
     2221 tggcgaaaat cagcgccccg atcagcacca cgccgcccag cacggcggtg ggaacgctcc
     2281 atttgttgag cgggatacgg aaaatcttga agatcgcgat acacagggcg gtgtacgtca
     2341 gaatcagtaa cagatccatt attgcgactc cccttcctgc gctacagatg tgactgcggc
     2401 gggcgctgcg acggcagccg ttctggattg ctccagcgca tcgatgcgcg cgcgcaggcc
     2461 cgccagttcg ttcatcagct catccttgcc cgacgcgcca tcggcaccgc caaaccccca
     2521 gccccggtcg gcgcggtaca gcgtggccca gatccacagg aacggccaga tcgcatgcaa
     2581 tgtcagcagg ctgacccagc ccgcaacatg gatggcatcc tggtgcggat gattgcggtg
     2641 cttcgcaatg tcgtagggaa tatcgtgaat cacaatgatc ccgtagaaga gggtgattgc
     2701 ggcaaatatc agaatgccga gcgcgacata ttccagcatg tagacttttc tccgccctga
     2761 gatcgggtcc gtggatggaa aaaccatacc catgatatac gttgcacggg cacggctaca
     2821 atcaaggctc ggacagcctt catcttgctg caaggaaacg gtaatgaaac agttgctgct
     2881 ggtcgcccac gcaccctccc ccaacaccgg cgccatggta caggctgtgc tgcgcggtgc
     2941 cggccatccg gacatcagcg gtgtacaggt tcgtcacata ccccccctgc aggcccagcc
     3001 ggacgacgtg ctgcaagccg acgcgctgat tctgggcacc acggaaaacc tgggttacat
     3061 gagcggggcg ctgaaggatt ttttcgaccg gatttattac ccctgcctgg aacagaccca
     3121 gggcaagccg ttcgcgtttt acattcgcgc cggccacgac ggcaccggca cccgccgcgc
     3181 catcgaatcc atcaccacgg ggctgcgctg gcgttgggtg caggaaccgt tgctatgccg
     3241 tggcccgtgg caggacagct ttctggagca gtgcgagcag ctgggaatga ccatggccgc
     3301 cagcctggag gccggcatca tctgatccac cactgcgctt cccgcatcag gccgcttcct
     3361 gcatttccac cgcgcccacg aacagcgccg cgatcaggtc ggactgggtg acgattccca
     3421 ccagccgccg ctcgtcgttc accaccggca catggtgcaa gcccttgtcc gacagcagcg
     3481 gcaccagcaa cgcaatgaga tcattttcat tcaccgtgaa cgcgggctgc gccatgatct
     3541 gccc
//
LOCUS       DASUEG010000305         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_421464, whole genome shotgun sequence.
ACCESSION   DASUEG010000305 DASUEG010000000
VERSION     DASUEG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421464"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="fmdA"
                     /locus_tag="VFX11_05670"
     CDS             <1..915
                     /gene="fmdA"
                     /locus_tag="VFX11_05670"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEX4938141.1"
                     /translation="FFSKKNGGGFLTEHFPEAQKSIWDFNGMFTKSRHVPGVEFAGLI
                     HPGLIGCLPSKEMLAEWNKREKELFDTDPTRVPGLANLPYAPTAHMGKMKKEDAAVAA
                     AEGARTVPPREHGGNCDIKDLSRGSKVYFPVYVKGGGLSVGDLHFSQGDGEITFCGAI
                     EMAGWIHLRVSLIKDGMKKYGIKNPIFKPSPITPKYDDYLIFEGISVDEQGKQHYLDV
                     HIAYRQACLNAIEYLTKFGYSRAQAYAILGTAPVQGHISGVVDVPNACATLWLPTDIF
                     EFDVKPNVDGPKKYLDGSIDVPLAPDLK"
     gene            949..1305
                     /locus_tag="VFX11_05675"
     CDS             949..1305
                     /locus_tag="VFX11_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4938142.1"
                     /translation="MPLYDYKCTSHGVFQELVPLAAFDQPCACPQCGTLSPRVILFSP
                     QFLAMAEERRKAFATNEKAQHEPVFSNREIREEASRHKHGKDCGCHAQQRKSNLLYTA
                     DGSKVFPSMRPWMISH"
     gene            complement(1412..1879)
                     /locus_tag="VFX11_05680"
     CDS             complement(1412..1879)
                     /locus_tag="VFX11_05680"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4938143.1"
                     /translation="MSNVYGSPGADLNQPLQDVEYVGFWMRVVASIIDNIWMFVLFFI
                     LAFVVVLATGLDESVLDSLMGTVLQIVLPFVIILGFWVKYAATPGKMVFRAKILDANT
                     LEPVPAGRLVLRYIGYFVCVLTLFIGFIWVGIDKRKQGFHDKIAGTVVVREKQ"
     gene            complement(1962..>2402)
                     /locus_tag="VFX11_05685"
     CDS             complement(1962..>2402)
                     /locus_tag="VFX11_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4938144.1"
                     /translation="TFSKSRSLAGLRVGFAVGQAKLIEGLERVKDSFNSYPLDRFALK
                     GAVAALEDEAYFVSTCERIMQTRAVLVQQLEQLGFEVVPSAANFVFARHPRHSADKIA
                     AALREKSIIVRHFKLPRIDQHLRITVGTDEECAALVKALVPVVS"
BASE COUNT          564 a          743 c          650 g          445 t
ORIGIN      
        1 tttttctcca agaaaaacgg cggcggattc ctcaccgaac attttcccga ggcgcagaaa
       61 tcgatttggg atttcaacgg catgttcacc aaatcgcgcc atgtgcccgg cgtcgaattc
      121 gccggcctga ttcaccccgg cctgatcggc tgcctgccgt caaaggaaat gctggcggag
      181 tggaacaagc gcgagaagga actgttcgac accgacccca cccgcgtgcc cggcctggcc
      241 aacctgccct acgcgcccac cgcgcacatg ggcaagatga aaaaggaaga tgcagccgtg
      301 gcagcagccg aaggcgcgcg caccgtgcca ccacgcgaac acggcggcaa ctgcgacatc
      361 aaggatttgt cgcggggttc gaaagtgtac ttccctgtct atgtcaaagg cggcggtctg
      421 tccgtcggcg atctgcactt cagtcagggc gacggcgaaa taacgttttg cggcgctatc
      481 gaaatggcgg gctggattca cctgcgggtc agcctgatca aggacggcat gaaaaaatac
      541 ggcatcaaga atccgatttt caaacccagc ccgatcacgc cgaaatatga cgattacctg
      601 atctttgaag gcatctcggt ggacgagcag ggcaagcagc attatctcga cgtgcacatt
      661 gcctaccgtc aggcctgtct gaacgccatc gaatatctca ccaaattcgg ttatagccgc
      721 gcgcaggcct acgcgattct gggcacagcg ccggtgcagg gtcatatcag cggtgtggtg
      781 gacgtgccca acgcctgcgc caccctctgg ctgccgacag atattttcga attcgatgtg
      841 aaacccaatg tcgacgggcc gaagaaatat ctcgatggca gcatcgatgt gccgctggcc
      901 cccgatctga aataaatgaa tcggaaacaa atagacggag gacaccccat gcccctgtac
      961 gactacaaat gcacaagcca cggcgtgttc caggaactgg tgccgctggc ggccttcgac
     1021 caaccctgtg cgtgtccgca atgcggcacg ctgtcacccc gggtgatcct gttctcgccg
     1081 cagttcctgg cgatggcgga ggaacggcgc aaggcgttcg ccaccaacga aaaggcgcag
     1141 cacgaaccgg tgttttccaa ccgggaaata cgggaggagg ccagcaggca caaacacggc
     1201 aaggattgcg gctgtcacgc gcagcaacgc aaatccaatc tgctgtacac cgccgacggc
     1261 agcaaggtat ttccgtcgat gcggccctgg atgatcagcc actgactgat agttgctgcg
     1321 ccgaaaaaaa acaaaaaccg cactgacagc ctcctgccgc gaactcgggg ctgtcagtgc
     1381 ggctttttgt ttcaggtatg tctaaaggtg cttactgttt ttcccgcacc accacggttc
     1441 cggcaatttt gtcgtgaaag ccctgcttgc gcttgtcgat acccacccag atgaaaccga
     1501 tgaacagcgt gagcacgcaa acaaaataac cgatgtagcg caataccaga cgacccgccg
     1561 gcaccggctc cagggtattg gcatccagta ttttcgcccg gaacaccatt ttgcccggcg
     1621 tagccgcata cttcacccag aagcccagga tgatcacaaa cggcagcact atctgcagca
     1681 cggtgcccat caggctgtcc agcacgcttt catccagtcc ggtcgccaga accactacga
     1741 acgccaggat gaaaaacagc acgaacatcc agatgttgtc gatgatggac gccaccaccc
     1801 gcatccagaa accgacgtat tcaacgtcct gcaggggttg attcagatcc gcgccaggcg
     1861 aaccgtacac attactcatt gcatgctcct tgatggattg acctgctctt gagtgtagtg
     1921 cgaaaaacgc aactcaaccg gtccgcttgg ggcgacgccc ttcagctcac caccggcacc
     1981 agcgctttaa ccagtgccgc gcactcttcg tcagtgccca cagtgatacg taaatgctgg
     2041 tcgatccgcg gcagtttgaa gtggcgcacg atgatggatt tctcccgcag cgccgccgcg
     2101 attttgtcgg cactgtggcg cgggtggcgg gcaaacacga aattcgctgc cgacggcacc
     2161 acctcaaaac ccagttgttc cagctgctgc accagcaccg cacgcgtctg catgatccgc
     2221 tcgcaggtgg aaacgaaata ggcctcgtcc tccagcgccg ccaccgcgcc cttcagcgcg
     2281 aaacggtcca gcggatagga attgaaactg tccttcaccc gctccagtcc ttcgatcagt
     2341 ttggcctgac ccacggcaaa acccacccgc aggccggcca gggaacggga tttggaaaag
     2401 gt
//
LOCUS       DASUEG010000306         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1769079, whole genome shotgun sequence.
ACCESSION   DASUEG010000306 DASUEG010000000
VERSION     DASUEG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769079"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /gene="hemH"
                     /locus_tag="VFX11_05690"
     CDS             <1..424
                     /gene="hemH"
                     /locus_tag="VFX11_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX4938145.1"
                     /translation="PDRLLLSFHGIPQRYEDQGDPYPSLCRATAKAVADRLGLADDQW
                     SESFQSRFGREEWIKPYTDVLLKEWGARDDINRVDVVCPAFSADCLETLEEIAVQNRA
                     LFLEAGGKDYRYIPCLNDRADHIDLLVQLVLRHSTPWR"
     gene            525..2324
                     /locus_tag="VFX11_05695"
     CDS             525..2324
                     /locus_tag="VFX11_05695"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4938146.1"
                     /translation="MELDGFSQLEKIAEGGMAVLFKGTQTSLSRPVAIKVMKGALANT
                     PEARELFEWESKIVARLDHPNIIRVIDKGLTREGMPYFVMDYVEGITLKDALKTGSLS
                     DRRKLRVIMQVAKALGYAHRNNIVHRDIKPGNILLDREGNARVVDFGIARLTEDTGLS
                     KVNEEGMTVGTPAYMAPEQHQGAAHTSSASDIYSLGVIMYLMLCGKLPRPGFPPPSQY
                     NPKVPAALDKITMACLAEDPGKRPNADKLVSLLLVALKGAHLKQEQKEEAKETFKDAK
                     DKFRLLDVIRETQWGSVSLYENREDRSLMVLKKRINSSKGFNEGELLSRLKHRNIANV
                     RGVSRNDRIFITVMDYLSGGSLQNRMAQPMPVVEFLPIADQICEAMIFAHNNRIIHGN
                     LRPHNILFDDTNTVKVMDFGFEPHYESMLDQNWYVADDNPSSVLGDVYAAGVIFYQIL
                     TGGLPEWKRGLLQPSNAFNDLPQHLRTLLKAMLCRDPSVRLQNFDEVKKGLRLQLEDM
                     DEKTRSQIKLAPPEETAPQRTRSRLLLPALGLLVLVNLGVYAWIYSSQSELPGWLGQW
                     LGRDNAAETNPSNTPRDDDGELTMQRVKGPKQN"
     gene            2440..>2747
                     /locus_tag="VFX11_05700"
     CDS             2440..>2747
                     /locus_tag="VFX11_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938147.1"
                     /translation="MIDYELTDLLIELEEIQQDRFLNLARGQQVVDLLGFYSIPEELY
                     VLRQEPQNGRQPHQCRRKIAIFNFAMITDEQKAPVIECLFMIGHSPEFIAEALGEDIR
                     "
BASE COUNT          618 a          913 c          745 g          471 t
ORIGIN      
        1 ccccgaccgc ctgctgctca gctttcacgg cattccccag cgctatgagg accagggcga
       61 cccctatccc agcctctgcc gtgccactgc caaggccgtg gccgaccggc tcggccttgc
      121 cgacgatcag tggtcggaaa gcttccagtc ccgcttcgga cgggaagaat ggatcaagcc
      181 ctacaccgac gtcctgctca aggaatgggg cgcccgcgac gacatcaacc gggtcgacgt
      241 ggtgtgcccc gccttctccg ccgactgtct ggaaacgctt gaggaaatcg ccgtgcagaa
      301 ccgcgcgctg ttcctggaag ccggcggcaa ggattaccgc tacataccct gcctgaacga
      361 ccgcgccgac cacatcgacc tgctggtgca actggtgctc cgtcacagca ccccctggcg
      421 ctgacattcc ggcgctgtca gagccgaaaa aagaccgcca gcgggacaaa tagcggctac
      481 ccttagcatt agtcgctatt tttcaatcga ttatcgatac cccaatggaa ttggacggtt
      541 tctcgcagct ggaaaagatc gccgaaggtg gcatggcggt gctcttcaag ggcacccaga
      601 cctccctcag ccgaccggtt gccatcaagg tcatgaaagg cgcgctggcc aacacgccgg
      661 aagcccgcga attgttcgag tgggaatcga agattgtcgc gcgcctggac catcccaaca
      721 tcatccgcgt catcgacaag gggctcaccc gcgagggcat gccctacttc gtcatggact
      781 acgtggaagg catcacgctc aaggatgcgc tcaagaccgg cagcctgtcc gaccggcgca
      841 aactgcgcgt catcatgcag gtggccaagg ccctgggcta cgcccaccgc aacaacatcg
      901 tccaccgcga catcaagccc ggaaacatcc tgctggaccg cgaaggcaac gcccgcgtgg
      961 tggacttcgg catcgcccgt ctcaccgaag acaccggcct gtccaaggtc aacgaagaag
     1021 gcatgaccgt gggcacccct gcctacatgg caccggagca gcaccagggc gccgcccaca
     1081 ccagttccgc cagcgacatc tattccctcg gtgtcatcat gtacctgatg ctctgcggca
     1141 aactgcccag gcccggtttc ccgcccccgt cacagtacaa cccgaaagtg cccgccgccc
     1201 tggacaagat caccatggcc tgtctggcgg aagaccccgg caaacgcccc aatgccgaca
     1261 aactggtctc gctgctgctg gtagcgctca agggcgcgca cctgaagcag gaacaaaagg
     1321 aagaagccaa ggaaaccttc aaggacgcca aggacaaatt ccgcctgctg gacgtgatcc
     1381 gggaaaccca gtggggcagc gtcagcctgt atgaaaaccg cgaagaccgc agtctgatgg
     1441 tgctgaaaaa gcgcatcaac agctcgaaag gcttcaacga aggcgaactg ctgtcacgcc
     1501 tgaaacaccg caacattgcc aacgtgcgcg gcgtcagccg caatgaccgc atcttcatca
     1561 ccgtcatgga ctacctgtcc ggcggcagcc tgcaaaaccg catggcccag cccatgccgg
     1621 tagtggaatt cctgcccatt gccgaccaga tctgcgaagc catgatcttc gcccacaaca
     1681 accgcatcat tcacggcaac ctgcgcccgc acaacatcct gttcgacgac accaacaccg
     1741 tcaaagtcat ggatttcggc tttgagcccc actatgaaag catgctggac cagaactggt
     1801 acgtggcaga tgacaatccc agcagcgtcc tgggcgacgt ctacgccgct ggcgtcatct
     1861 tctaccagat cctgaccggc ggcctgccgg agtggaaacg cggcctgctg caacccagca
     1921 acgctttcaa cgacctgccg cagcacctgc gcacgctgct caaggccatg ctctgccgcg
     1981 accccagcgt acgcctgcag aatttcgacg aagtgaagaa gggcctgcgg ctgcagctgg
     2041 aagacatgga cgaaaagacc cgcagccaga tcaaactggc ccctccggaa gaaacggcac
     2101 cgcagcgcac ccgctcgcgc ctgctgctgc cagcgctggg cctgctggtc ctggtgaacc
     2161 tcggcgtcta tgcctggatc tacagcagcc agtccgagtt gccaggctgg ctcggccaat
     2221 ggctgggccg tgacaacgct gcagagacca acccttcgaa caccccccgg gacgacgacg
     2281 gcgaattgac gatgcagcgg gtcaagggcc cgaaacagaa ttagtcttgt ttgtttattc
     2341 gtctgttcaa tctcgcgcta aatatgctag cttaatccgc cagaatgtat actttggtgc
     2401 attaagggac tgcgcgcgtc acacccgatg gaggattcca tgattgacta cgaactgact
     2461 gacctgctga ttgagctgga ggaaatccag caagatcgct tcctgaacct ggcccgcggg
     2521 caacaggttg ttgatttact tggcttttac agcattcccg aagaactcta cgtactgcgg
     2581 caagaacccc agaacggtcg ccagccccac cagtgccgac gcaagattgc catcttcaac
     2641 ttcgccatga tcactgacga gcagaaggcc cccgtgatcg agtgcctgtt catgatcggg
     2701 cattcccccg agttcattgc cgaagccctg ggcgaggaca tccgctt
//
LOCUS       DASUEG010000307         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1083997, whole genome shotgun sequence.
ACCESSION   DASUEG010000307 DASUEG010000000
VERSION     DASUEG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083997"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFX11_05705"
     CDS             <1..660
                     /locus_tag="VFX11_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4938148.1"
                     /translation="VRLFMMFAAPPEQSLEWSDSGVEGANRFLRKLWRMVAAHLEKGA
                     APALDTSALNEQQKDLRRKLHETIAKVRDDFERRYTFNTAIAAVMELSNAMAKLDDDG
                     AQSRAVLQEAIEACVLMLAPITPHIAHRLWAELGKIGTVLDAPWPVVDESALVRDSIE
                     VVVQVNGKLRGKIQVAANAGEDDVKAAALANEAALKFMDGKTVRKVIYVPGKLVNIVV
                     G"
     gene            671..1375
                     /gene="lptE"
                     /locus_tag="VFX11_05710"
     CDS             671..1375
                     /gene="lptE"
                     /locus_tag="VFX11_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX4938149.1"
                     /translation="MPAESAKTTSAMKITGSIWILSLTLLLAACGFQLRGVEHSINSR
                     YESIRLTTNGKDEQFHATLRKSLKNAGVTLSENADARLEILGTKMEKRTASYSSRAKS
                     AEFELLKTVSFRFRDNGRELISDMPLQARRSYLYRETAAVGKAEEENLLWQEMDLDLS
                     QRILMALQRTVADGGKMPVAEYPAEEDSPDTAAPATNPAENVPVDATVPDAPAQESAQ
                     PSTQQPPATSPQEAAP"
     gene            1372..2418
                     /gene="holA"
                     /locus_tag="VFX11_05715"
     CDS             1372..2418
                     /gene="holA"
                     /locus_tag="VFX11_05715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX4938150.1"
                     /translation="MKLRPNQVKEHFAKGPLLPLYILSGDEPLLMQETADQIRAEARK
                     AGFSERELFTVENNFDWDNVLQSGNSMSLFGDRKILEVRNEKGKFDDAAKKALQIYCE
                     NPNPDNLLLLIIPKVDKKTQTAKWFTQLETIGAFIQVWPVDAQQLPQWIHQRMRTANL
                     EPTREAVQLLAERVEGNLLAAAQEVEKLILLRGEGPLDVRDIEDAVADHARYNLYDMV
                     DEALQGNAAHAIRMLNYLRASGTEPPVLLWAITKELRTLAGVSHLIDTGLAPARALQE
                     YRVWDNRKDLMQNVLKRLPGRAFKHCLLEAARIDQAVKGLGEGDPWDGFTNIILWLSG
                     RIKPGLMALDAPSA"
     gene            2462..3031
                     /locus_tag="VFX11_05720"
     CDS             2462..3031
                     /locus_tag="VFX11_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HEX4938151.1"
                     /translation="MTSQTTRTPTALVPIADGSEDIESVTIIDVLRRAGVNVTVASVA
                     PDARLQITAARGTRIVADTHIEQVAQQAFDLIALPGGMPGATHLRDCATLIGLLKQQR
                     QAGKWYAAICASPAVVFASHKLEDDVAATAYPSFTSQLHHFRNERTVVDKNCITSQGP
                     GTALEFALTLVEVLLGREKREQVGSPMVL"
     gene            complement(3045..>3937)
                     /locus_tag="VFX11_05725"
     CDS             complement(3045..>3937)
                     /locus_tag="VFX11_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938152.1"
                     /translation="ACYAAKEGGRNRIHVYRQDDKELEQRRGEMQWVTRIPRGIRENR
                     FALAIQEIFATDAASVRERHVELLIRYRRQDGQWIAPRMFLPAAERYNMATMLDRWVF
                     VQLANLLQQQPQLVEQGWVFNINLSGQSVTNDEFLQFIIERMTELKLPTAGICFEITE
                     TSAITNLHRAQHFIGSLKALGCRFALDDFGSGLSTFGYLKGLPVDYIKIDGQFVRDIE
                     KDAIDFAIVKSINEVGHVLGKQTMAECVENAEIARMLAAIGVDWMQGFQLAQPLMLNE
                     WLQRISLVELGELPNQDQAG"
BASE COUNT          863 a         1224 c         1174 g          676 t
ORIGIN      
        1 gtgcgcctgt tcatgatgtt cgccgctccg ccggagcagt cgctggagtg gtctgacagc
       61 ggcgtggaag gcgccaaccg cttcctgcgc aaactctggc gcatggtggc cgcgcatctg
      121 gaaaaaggcg ctgcgccggc actggacacg tccgcgctga atgagcagca aaaagatctg
      181 cgccgcaagc tgcatgaaac catcgccaag gtgcgcgatg atttcgaacg ccgttacacc
      241 ttcaacacgg ccattgccgc cgtgatggaa ctgagcaacg ccatggcgaa actggacgac
      301 gacggtgcgc agagccgcgc ggtgctgcag gaagccattg aagcctgcgt gctgatgctg
      361 gcgccgatca caccgcacat cgcgcaccgg ctgtgggcgg aactgggcaa gattggcacc
      421 gtgctggatg caccctggcc cgtcgtggat gaatccgcgc tggtgcgcga cagcatcgaa
      481 gtggtagtgc aggtgaacgg caagctgcgg ggcaagatcc aggtggccgc caacgccggc
      541 gaagacgacg tgaaggcagc ggcactggcc aacgaggccg cgctgaaatt catggacggc
      601 aaaaccgtgc gcaaggtgat ctatgtgcca ggcaagctgg tgaatattgt tgttggatga
      661 gcacgcaacc atgcccgctg aaagcgccaa gacaacgtcc gccatgaaaa tcaccggcag
      721 catctggata ctgtccctca cgctgttgct ggccgcctgc ggctttcagc tgcggggcgt
      781 ggagcattcc atcaacagcc ggtatgaaag cattcgcctg accaccaatg gcaaggacga
      841 acagttccac gccaccttgc gaaaaagtct gaaaaatgcc ggtgtaacat tgagtgagaa
      901 cgccgatgca cggctggaaa ttctcggcac caaaatggag aagcgcaccg cgtcctacag
      961 cagccgggcc aagagcgccg aatttgaact gctgaaaacc gtctccttcc gcttccgcga
     1021 caatggccgg gagctgatca gcgacatgcc tctgcaggca cggcgatcct acctgtatcg
     1081 ggaaacggcc gcagtgggca aagccgagga agaaaacctg ctgtggcagg aaatggacct
     1141 ggacctgtca cagcggattc tgatggcact gcaacgcacc gtggccgacg gcggcaagat
     1201 gcctgttgca gagtatcctg cggaagagga ttcgcccgac actgcagcac ccgccaccaa
     1261 ccccgctgaa aacgtccctg tcgatgcaac ggtgcctgac gctccagcgc aggaatccgc
     1321 acagccgtcc acacaacagc caccagcaac ctcgccccag gaagctgcac catgaaactg
     1381 cggcccaacc aggtgaagga acatttcgcc aaggggccgc tgctgccgct gtatatcctg
     1441 agtggcgacg aaccgttgct gatgcaggaa accgccgacc agattcgcgc cgaggcgcgc
     1501 aaggccggtt tcagcgagcg cgagctgttc acggtggaaa acaatttcga ctgggacaat
     1561 gtgctgcagt ccggcaacag catgtcactg tttggtgacc gcaagatcct ggaagtgcgc
     1621 aacgagaaag gcaagttcga cgatgccgca aaaaaggcgt tgcaaatcta ttgcgagaat
     1681 cccaatcccg acaacctgct gttgctgatc attcccaagg tcgacaagaa aacccagacc
     1741 gcaaaatggt tcacgcagct ggagaccatc ggcgccttca tccaggtgtg gccggtggac
     1801 gcgcagcaac tgccgcaatg gattcaccag cgcatgcgca ccgccaatct ggagcccacc
     1861 cgcgaagcgg tgcaactgct ggcggaacgg gtggagggca atctgctggc cgctgcgcag
     1921 gaagtggaaa aactgattct gctgcgcggt gaaggcccgc tggatgtgcg cgacatcgaa
     1981 gacgcagtag ccgatcacgc acgctacaac ctgtacgaca tggtggacga agcattgcag
     2041 ggcaacgccg cccacgccat ccgcatgctg aattatctgc gcgccagcgg caccgagccg
     2101 ccggtgttgt tgtgggccat caccaaggaa ctgcgcacgc tggccggcgt gtcgcacctg
     2161 atcgacaccg gcctggctcc cgcccgcgca ctgcaggaat accgggtgtg ggacaaccgc
     2221 aaggatctga tgcagaacgt gctgaagcgt ttgccagggc gtgcattcaa gcattgcctg
     2281 ctggaagcgg cccgcatcga tcaggcggtg aagggattgg gcgaaggcga tccctgggac
     2341 ggcttcacca acatcattct gtggctgtcg gggcggatca aaccgggcct gatggcactg
     2401 gacgcaccgt ccgcctgaca cagcatccga aatagcagca acgccagcaa ggggaattcc
     2461 catgaccagt caaaccaccc ggacacccac cgcactggtt cccatcgccg acggctcgga
     2521 agacatcgaa tccgtcacca tcatcgacgt gctgcgccgc gccggcgtga acgtcaccgt
     2581 ggccagcgtg gcacctgacg cgcgcctgca gatcaccgct gcgcgtggca cccgcatcgt
     2641 cgccgacacg cacatcgaac aggtcgcaca acaggcattc gatctgatcg cgctgcccgg
     2701 cggcatgccc ggcgccaccc acctgcggga ttgcgcgacg ctgatcgggc tgctgaaaca
     2761 acagcggcag gcgggaaaat ggtacgccgc aatctgtgcc agccccgcgg tggtgttcgc
     2821 cagtcacaaa cttgaagacg atgtcgcggc caccgcgtat ccctccttca cgagtcagct
     2881 gcatcatttc cgcaacgagc gcacggtggt ggataaaaac tgcatcacca gccagggtcc
     2941 tggtacggcg ctggaatttg cactcacact ggtggaagta ttgctgggac gggaaaagcg
     3001 cgaacaggtc ggctcgccga tggtgttgta acgcacctgt attttcagcc tgcctgatcc
     3061 tgattcggca attcaccgag ttccaccagg ctgatccgct gcagccactc attcagcatc
     3121 agcggctgcg ccagctggaa accctgcatc cagtccacgc cgatcgccgc cagcatgcgg
     3181 gcaatttccg cgttctccac gcactccgcc atcgtctgct tgcccagcac atggcccact
     3241 tcgttgatgg atttgacgat ggcgaaatcg atggcgtcct tttcgatatc gcgcacgaac
     3301 tggccgtcga tcttgatgta atccaccggc aatcctttca gataaccgaa cgttgacagg
     3361 ccgctgccga aatcgtccag cgcgaaacgg caacccagcg ctttcagact gccgatgaaa
     3421 tgctgggcgc gatgcaggtt ggtgatggcc gaggtttcag tgatttcaaa acagatgccg
     3481 gcggtgggca gtttcagttc ggtcatgcgc tcgatgatga actgcaggaa ttcatcattg
     3541 gtgaccgact gccccgacag attgatattg aacacccagc cctgctccac cagctgcggt
     3601 tgctgctgca acagattcgc cagctgcacg aacacccagc gatccagcat ggtggccatg
     3661 ttgtagcgct ccgctgccgg caggaacatg cgcggcgcga tccactggcc gtcctggcgg
     3721 cggtagcgga tcagcagctc cacgtgccgt tcgcgcacgc tcgcagcatc ggtggcaaaa
     3781 atctcctgga tcgccagcgc aaaccggttc tcgcgaatgc cccggggaat ccgcgtcacc
     3841 cactgcattt cgccgcgccg ctgttccagc tccttgtcgt cctggcgata gacatgaatg
     3901 cggttgcggc ctccttcctt cgctgcatag caggcgg
//
LOCUS       DASUEG010000308         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_605952, whole genome shotgun sequence.
ACCESSION   DASUEG010000308 DASUEG010000000
VERSION     DASUEG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605952"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VFX11_05730"
     CDS             complement(<1..94)
                     /locus_tag="VFX11_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX4938153.1"
                     /translation="MKLVVISGRSGSGKTTALHVLEDIGYYCVDN"
     gene            complement(120..572)
                     /gene="ptsN"
                     /locus_tag="VFX11_05735"
     CDS             complement(120..572)
                     /gene="ptsN"
                     /locus_tag="VFX11_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX4938154.1"
                     /translation="MELAEIITPERVFDHVTGASKKKILETAAGLIARQFPELDPNEL
                     FDSLVAREKLGSTGLGKGIAIPHCRATHCGSAIGTFIKLESPVDFDAIDRQPVDMLFV
                     LLVPKAAHEEHLNILAKIATLLNDDRRRETIRRAPSPQAIYDALVTPL"
     gene            complement(603..890)
                     /gene="raiA"
                     /locus_tag="VFX11_05740"
     CDS             complement(603..890)
                     /gene="raiA"
                     /locus_tag="VFX11_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX4938155.1"
                     /translation="MQINISGHHVEVTDALKDYVADKLQKLERHTGDITSIQAILSVE
                     KNRQKAEATIRVKGADLFANAESEDMYAAIDMLTDKLDRQVLKHKEKMKSW"
     gene            complement(1024..2649)
                     /locus_tag="VFX11_05745"
     CDS             complement(1024..2649)
                     /locus_tag="VFX11_05745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX4938156.1"
                     /translation="MKPSLQLKIGQHLTMTPQLQQAIRLLQLSTLDLQAEIQEALDSN
                     PLLEMEERSSEDSDGGPEADAPVVEADAPGEFNDLIDFAKTTDYTSSSDTPVTQHEDE
                     TSVDLNNKEMPDELPVDSDWDTMFDGTAGSSSGSHDDDDYDFEGRNSAPESIYDNLLW
                     QLNLTHMSDLDRVIAMAIIEAIDTKGYLSQTVEEIRDCVLAQKDVVADIIALQQQELE
                     AFGVQLHHDEGEEETPVTLEEVLAVLHRIQQFDPPGVAARDLRECLLIQLRQLPKSPD
                     REDALLLVEMFLEALGARDFTLIMRKTKLTENRLKTAIALIQTLNPYPGSSVEADETQ
                     YVIPDVIVRKDKGRWRVELNPEATPRLKVNSSYASLIQRADNSSDNTFLKNNLQEARW
                     FIKSLQSRNETLLKVATKIVEHQIGFLEYGEEAMKPLVLHDIAEAVSMHESTISRVTT
                     QKYMHTPRGVFELKYFFSSHVSTGSGGECSSTAIRAIIKKLISAENPKKPLSDSKIAD
                     ILAEQDIQVARRTIAKYREAMLIPPSNERKRLV"
     gene            complement(2838..>2945)
                     /locus_tag="VFX11_05750"
     CDS             complement(2838..>2945)
                     /locus_tag="VFX11_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX4938157.1"
                     /translation="AYIVSEGHIIAEGEPSLILQNQKVRDVYLGQQFQM"
BASE COUNT          500 a          794 c          897 g          754 t
ORIGIN      
        1 gattgtcgac acagtaatag ccgatatctt ccagcacatg cagcgccgtg gttttgccgg
       61 agccggagcg gccgctgatg accaccagtt tcatgcccgg attgctcctt tgcagactgt
      121 cacagcggcg tcaccagcgc gtcatagatc gcctggggtg acggcgcgcg gcgaatggtt
      181 tcgcggcggc gatcatcgtt cagcagcgtg gcgatcttgg ccaggatgtt gagatgttcc
      241 tcatgcgcgg ccttgggcac cagcagtacg aacagcatgt ccaccggctg gcgatcgatg
      301 gcgtcgaaat ccaccgggct ttccagcttg atgaaggtgc cgatggcgct gccgcagtgc
      361 gtggcgcggc aatggggaat ggcgatgccc ttgcccagtc cggtgctacc caatttttca
      421 cgggccacca ggctgtcgaa cagttcattc gggtccagtt cgggaaactg ccgggcaatc
      481 agcccggcag cggtttcgag gattttcttt ttgcttgcgc ccgtcacatg gtcgaaaacc
      541 cgttcggggg tgatgatctc agctagttcc ataaggtttt actgtgcagg aggaaggaag
      601 gcttaccagc ttttcatctt ttccttgtgc ttgagcactt gccggtccag cttgtcggtc
      661 agcatgtcga tggcagcata catgtcctcg gattcagcgt tggcgaacag gtcggcgcct
      721 ttcacccgga tggtggcttc tgctttctgg cgattctttt ccacgctcag gattgcctgg
      781 atgctggtga tgtcgccagt gtggcgctcc agcttctgca gcttgtcggc aacatagtcc
      841 ttcagtgcat cagtcacttc tacgtggtgt ccgctgatgt tgatttgcat aggccactcc
      901 ttaatcgtgg ttgcgttttc atgcttctgc gggtcggaac attgctgtcc cggtattgga
      961 aactttccgc cagtttgacg gtgggtgcaa gtggaatatt gatccaccgc atcccgtacg
     1021 tgattacacc agccgcttgc gttcgtttga cggcggaatg agcatggcct cacggtattt
     1081 ggcgatggtg cggcgggcaa cctggatgtc ctgctcggcc aggatgtcgg cgatcttgct
     1141 gtcgctcagg ggtttcttcg gattttcggc cgaaatcagc ttcttgatga tggccctgat
     1201 ggcggtggac gaacactcgc cgccggaccc ggtgctgaca tggctggaga agaaatattt
     1261 cagttcgaat acaccccgtg gcgtgtgcat gtatttctgg gtggtgacac gggagatcgt
     1321 ggattcgtgc atgcttaccg cttcggcaat gtcgtgcagc accagcggtt tcatggcctc
     1381 ttcaccatat tccagaaagc cgatctggtg ttcgacgatc ttggtggcca ctttcagcag
     1441 ggtctcgttg cggctctgca agcttttgat aaaccagcgt gcctcctgca gattgttttt
     1501 caggaaggtg ttgtcgctgc tgttgtcggc ccgctggatc agcgaggcgt agctgctgtt
     1561 cactttcagt cggggggtgg cttccgggtt cagttccacc cgccagcgtc ccttgtcctt
     1621 gcgcacgatg acatccggta tcacatactg ggtttcatcg gcttcgacac tgctgccggg
     1681 ataagggttc aatgtctgga tcagcgcgat cgccgttttc aggcggtttt cggtgagttt
     1741 ggtcttgcgc atgatcaggg tgaaatcacg ggcacccagg gcttccagga acatctccac
     1801 cagcagcagg gcatcttccc ggtccggact tttgggcagc tggcgcaact ggatcagcag
     1861 gcattcgcgc aggtcgcgcg cggccacgcc aggcgggtcg aactgctgga tgcggtgcag
     1921 cactgccagc acttcctcca gtgtcaccgg ggtttcttct tcgccttcgt catggtgcag
     1981 ctgaacgcca aaggcctcca gttcctgctg ctgcagggcg atgatgtccg ccaccacatc
     2041 tttctgcgcc agcacgcagt cgcggatttc ctccacggtc tggctcaggt agcccttggt
     2101 gtcgatggct tcgatgatgg ccatggcgat cacccggtcc aggtcggaca tgtgggtcag
     2161 gttcaattgc cacagcaggt tgtcgtagat ggattcagga gcactgttgc ggccttcgaa
     2221 gtcgtaatcg tcgtcatcgt ggctgccgga ggatgaaccg gccgtgccgt cgaacatggt
     2281 gtcccagtcg ctgtcgaccg gcagttcgtc gggcatttcc ttgttgttga ggtcgacgga
     2341 ggtttcgtct tcgtgctggg taaccggggt gtcgctgctg gaggtgtaat cggtggtttt
     2401 ggcaaaatcg atcaggtcgt tgaattcgcc gggggcgtcg gcttccacca ccggggcgtc
     2461 ggcttcgggg ccgccgtcac tgtcttcgct actgcgctct tccatctcca gcagcgggtt
     2521 ggaatccagc gcttcctgga tttccgcctg cagatcgagg gtggaaagct gcagcagacg
     2581 gatggcctgc tgcagctggg gggtcatggt gaggtgttgg ccaattttta gctgtaaaga
     2641 cggcttcatt gatacctgtt tactttacgt ccggaatacg tggggtccac ctgcgcgacg
     2701 actgcgccct gtttgaaacg ctgccaaatt gcgctcaatt tcaatgagtt actgggcagc
     2761 gcgaacgtcc tttcctttca attgtaagag cgaagcggcg gcaaagcaat ttgcatgcct
     2821 gaaatacttt agaactttta catctggaat tgctggccca ggtaaacgtc gcgcactttc
     2881 tggttttgca ggatcaatga gggttccccc tcagcgatga tgtggccttc gctgacgata
     2941 taggc
//
LOCUS       DASUEG010000309         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_437552, whole genome shotgun sequence.
ACCESSION   DASUEG010000309 DASUEG010000000
VERSION     DASUEG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437552"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..815
                     /locus_tag="VFX11_05755"
     CDS             237..815
                     /locus_tag="VFX11_05755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX4938158.1"
                     /translation="MQPIVLGSSSVFRRQLLEKLLDQFDTCSPDIDESPHPGESPTAL
                     VQRLALEKAQAVAKAHPTALIITSDQVSVVDGRINGKPGNYENAFRQLKNSSGKQVSF
                     FTSLCLYDATTAKYQLDVEPFQVHFRALSDAQIERYLKKETPYNCAGGFKSEGFGITL
                     FSRLEGRDPNTLIGLPLIRLVEMLAAAGVQLP"
     gene            926..1561
                     /locus_tag="VFX11_05760"
     CDS             926..1561
                     /locus_tag="VFX11_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938159.1"
                     /translation="MSEDLALNKPRRRNTKEKIIDEAESLAALHGIEHLKLQDVADKI
                     GIKLPSVYAHFSGREDILAAMGARISKGIGQLYIARTGETPIETLKRSAQDLLKMLGE
                     HPAYFRLMLRDHSVPMGYDPLNHRAAVNQNFALPSDIRDMLVRLQHLIDKSCKDGVKL
                     SAEDFAGCVHGFMLVRLSWLPTRVSEDGHVYVDYTLLQSTLDQIINRLLCV"
     gene            complement(1654..2559)
                     /locus_tag="VFX11_05765"
     CDS             complement(1654..2559)
                     /locus_tag="VFX11_05765"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX4938160.1"
                     /translation="MVMSAQDEQRKSRRWGIFFKLLTLGYLVVALLVFSAGKWSDGMP
                     NSSKDHTALVDLNGIIMADSEASADRVVKGLREAFKAEHAKGVVLRVNSPGGSPVQSG
                     MVYREIRRLKQEHPDKKIYAVITDAGASGAYYISAAADEIYADPASIVGSIGVVSDGF
                     GFVNAMEKVGVERRVYTSGDNKAMLDPFQPVDEQQKAYFSSILGDVHQQFIDAVKEGR
                     GDRLKDDPTIFSGLFWSGRQALELGLIDGLASPGQVAREMIGQEEIVDYTPQVNPFDA
                     FVGKLGVSMADRFMAMLGIGNIRLQ"
     gene            complement(2710..3369)
                     /locus_tag="VFX11_05770"
     CDS             complement(2710..3369)
                     /locus_tag="VFX11_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4938161.1"
                     /translation="MTPFQLVVFDWDGTLMDSLARIVASMQAAAIDLGIEPRSRDAVH
                     DIVGLALDDAISRLHPGLNQEGIRHMHDRYAHHYVEGDKTPSPFFEGARELLQDLYDG
                     GVLLSVATGKSRKGLDRIMQAHRVEPLFHSSRCADETRSKPEPDMLLEILDYHGLQPG
                     QAVMIGDTEFDLEMASRAGVPSVGVTWGAHDITRLRRHQPRVCVDSVSELSNWLRNPT
                     V"
     gene            complement(3384..4331)
                     /gene="rluC"
                     /locus_tag="VFX11_05775"
     CDS             complement(3384..4331)
                     /gene="rluC"
                     /locus_tag="VFX11_05775"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HEX4938162.1"
                     /translation="MSTNDAPVKVQQIEVSADQAGQRIDNFLVTRLKGVPKSAIYRMI
                     RKGEVRVNKGRIKPEYKLELGDTVRIPPARTAQRDETPLFIGDQLKTQLEAAILFEDG
                     GLIVLNKPSGLAVHGGSGLSLGVIEALRVIRPQQKFLELVHRLDRDTSGLLLVAKKRS
                     VLRQLHDDLREGRVEKTYLALLYGRWNGRQHRIEAPLKKFDLASGERVVRVSQEGKPS
                     LTLFEQKRLFEQATLVEAKPVTGRTHQIRVHAQFAGHPIAGDDKYIPREQDALFREKG
                     LKRLFLHAFRIEFNDSEGKRLSFEAPLPADLQALLNKLQ"
BASE COUNT         1038 a         1404 c         1275 g         1035 t
ORIGIN      
        1 gaagcccttc agtttgccct gctggcgagc aagcttgagg ggtttaatgg tagacggcag
       61 agggtgctca aacataagcg cgccattcta ttgacaaggt tttgacctgt caaagcattt
      121 gacagggttt ttacgggctg gaagcgcatt ttccgttatg cgaacatgcg cccccgacgg
      181 tcgggtggca aacctgcccc gttttcactt accccctgat ttttctggag ttccctgtgc
      241 agccaatcgt cctgggttca agttccgtgt ttcgccggca attactggaa aagctgctcg
      301 accagttcga cacctgctcg cccgatatcg acgaatcccc ccaccccggc gaaagtccga
      361 ccgcactggt acaacgtctt gcactggaaa aagcgcaggc ggttgcgaaa gctcatccca
      421 cagccctcat cattaccagc gaccaggtgt cggtcgtcga tggccggatc aacggcaaac
      481 ccggcaatta cgaaaacgca tttcgacaat tgaaaaacag cagtggaaaa caggtctctt
      541 tttttaccag cctctgtctc tatgatgcga ctacggcaaa gtatcaactg gatgtcgaac
      601 cgtttcaggt tcacttccgt gcgctgagcg atgcccagat tgagcgatat ctgaaaaaag
      661 aaaccccgta caactgtgct ggtggtttca agtccgaagg ttttggaatc acactcttca
      721 gccgcctgga aggacgtgat cctaacacgt tgattggatt acccctaatc aggttagtgg
      781 aaatgcttgc tgctgcgggg gtccagttac cgtaattttt tttgttaatt tctgaacaat
      841 cttcggctga cgtctacact tataatgtta ggttcaacgg catgcgagca gtagtcgtat
      901 gccatgagtg acgaggcaat tccgaatgtc cgaagatttg gcactgaaca aaccccggcg
      961 cagaaacacc aaggaaaaaa tcatcgacga agctgagtcg cttgctgcgc tgcacggtat
     1021 cgaacatctg aaactgcagg acgtggcgga caagatcggc atcaagctgc cgtctgtcta
     1081 cgcacatttc agtggtcgcg aagatattct ggccgccatg ggcgcacgca tcagcaaagg
     1141 catcggacag ctttatattg cgcgcactgg cgagacaccc atcgaaacac tcaaacgcag
     1201 cgcgcaggat ctgctgaaaa tgctgggcga acatccagct tattttcgtc tgatgctgcg
     1261 tgatcactcg gtgccgatgg gatacgatcc gctgaatcac cgcgcggcgg tgaaccagaa
     1321 ttttgcattg ccgagcgata tccgcgacat gctggtaagg ctgcaacatc tgatcgacaa
     1381 atcctgcaag gacggcgtca aactgtcggc cgaagatttt gcgggctgcg tgcacggttt
     1441 catgctggtg cgtttgagct ggctgcccac tcgggtgtcc gaagatggac atgtctatgt
     1501 ggattacacc ctgctgcaat caacgctcga ccagatcatc aaccggttgc tgtgcgtctg
     1561 atttcaacat tctttttttc gcacaacaaa aaaggcagcg atcgattcgc tgcctttttt
     1621 gttgtgccca ggtgacgctt gcgcgactag ccgtcactgc aaccggatat tgccaattcc
     1681 cagcatcgcc atgaaccgat cagccatgct gactccgagc ttgccgacaa aggcatcgaa
     1741 tggattgacc tgaggcgtat agtccacgat ttcttcctga ccaatcatct cgcgcgccac
     1801 ctgacccggg ctggccaggc catcgatcag acccagctcc agcgcctgcc ttcccgacca
     1861 gaacagtccg gagaatatgg tgggatcatc cttcaggcga tcgccccgcc cttccttcac
     1921 cgcatcaatg aactgctggt gaacatcacc caggatcgag gaaaaatacg ctttctgctg
     1981 ctcatccacg ggctgaaagg ggtccagcat ggccttgtta tcgccggacg tgtagacacg
     2041 gcgttcaacg cccactttct ccatcgcatt cacaaaacca aaaccgtcgc tgaccacacc
     2101 gatggagccc acgatactgg ccggatcggc atagatctca tcagcggcag ccgagatgta
     2161 ataagctccg gaagcaccgg catccgtaat caccgcatag atcttcttgt ccggatgctc
     2221 ctgcttcagg cggcggattt cacggtaaac catgccggac tgtaccgggc tgccgccggg
     2281 gctgttgacc cgcagcacaa cgcctttggc gtgctcagcc ttgaaggctt cacgcaggcc
     2341 cttcaccaca cggtccgcac tggcctcgga atccgccatg atgatgccgt tcagatccac
     2401 cagcgccgta tgatccttgc tgctgttggg catgccgtcg ctccacttgc cggcactgaa
     2461 caccagcagg gccaccacca ggtaacccag cgtcagcagc ttgaaaaaga tcccccaacg
     2521 ccgggatttg cgctgctcgt cctgggcgga catcaccatg cgctcgatca gcttccattc
     2581 cttgttggac ttcggccgat tgaaattgct gcgactgcga tcgcggtcgt tacggttggg
     2641 tatattctga ttctgatcca tttaacctac ctttattgaa ctgctttcaa ctgaaccagc
     2701 ggcattgcgt cagaccgtcg ggttgcgcaa ccagttcgac aactcagaga cggaatcgac
     2761 gcaaacgcga ggctggtgac gccggagccg tgttatatca tgagcccccc aggtcacacc
     2821 aaccgacggt acgccggcac ggctcgccat ctccagatcg aactcggtgt cgccgatcat
     2881 gacggcctgg cccggctgca atccatgata gtcgagaatt tccagcagca tgtccggctc
     2941 gggcttggag cgggtttcgt cggcgcagcg ggagctgtgg aacagcggct cgacccggtg
     3001 ggcctgcatg atgcgatcga gccccttgcg acttttgcca gtggccacgc tgagcagcac
     3061 gccaccatca tacagatcct gcagcagctc cctcgccccc tcaaagaacg ggctcggggt
     3121 cttgtccccc tccacataat gatgggcgta gcggtcgtgc atatgacgga taccttcctg
     3181 gttcaacccg ggatgcagcc gcgagatcgc atcgtccagg gccaggccaa cgatgtcgtg
     3241 caccgcgtcc cgggaacggg gctcgatgcc caggtcgatg gcggccgcct gcatgctggc
     3301 cacaatacgg gccagggagt ccatcagggt gccgtcccag tcgaatacca ccagttggaa
     3361 gggtgtcatg ctcgtataac gtctcactgc aatttgttca gtagggcctg caggtctgca
     3421 ggcaggggtg cctcaaaact cagccgcttg ccttcactgt cgttgaactc gatacgaaag
     3481 gcatgcagga acagccgctt caggcccttt tcccggaaca gggcatcctg ctcgcgggga
     3541 atatacttgt cgtcacccgc gatggggtga ccggcaaact gggcatgcac ccggatctgg
     3601 tgggtacgcc cggtgaccgg cttggcctcc accagcgtgg cctgctcgaa cagacgcttc
     3661 tgctcgaaca gggtcagaga cggcttgcct tcctgggaca cccgcaccac ccgttcgccc
     3721 gaggccaggt cgaacttctt cagcggcgct tcgatgcgat gctggcgacc gttccagcgg
     3781 ccatacaaga gtgcgagata ggttttctca acccgtcctt cccgcagatc atcgtgcaac
     3841 tggcgcagca cactgcgttt cttcgccacc agcaacaggc cggacgtatc ccggtccaga
     3901 cggtgcacca gctccagaaa cttctgctgg ggccggatca cccgcagggc ttcgatcacc
     3961 cccaggctga gtccgctgcc accatgcacg gcgagtcccg acggcttgtt gagcacgatc
     4021 aggccgccat cctcgaacaa aatggcggct tcaagctggg ttttcaactg gtcaccaata
     4081 aacagcggtg tctcgtcccg ctgggcggta cgggcgggcg ggatgcggac ggtatccccc
     4141 agttcgagct tgtattcggg cttgattcgt cccttgttga cccgcacctc tcctttgcga
     4201 atcatgcggt aaatggctga tttcggaaca cctttaaggc gggtgacaag gaagttgtcg
     4261 attcgctgcc cggcctggtc ggcactgact tcgatttgct ggacttttac gggcgcgtca
     4321 ttcgtgctca tgaagaatca agaggttatc gctatctgtt tgatggggct aacaattcct
     4381 gctatattcc gaagtgcctc aaaaagcatg catggcttag cagggtaaca ttgaccggta
     4441 accggcctaa attgagtttc gtccggcaaa cagccggtcc ccaaaaggga cgaccattct
     4501 actcaggtta cagtcgatca cctaattctc atgcatcctt gattcaggca ccggaatcgg
     4561 gaccaggtcg caattggcag tcagcccgat tccagaacgc acgtttaaat accaatgatg
     4621 gtacggtggg cacacattgc tgcaccgtga acgttaaaca agcgtctgac gtagaaaagc
     4681 gaacgattac tgtatatcga gcctgattcg aacgcaccgg caggcatcgc gcaatggcat
     4741 ctcacagcgc ga
//
LOCUS       DASUEG010000310         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_250653, whole genome shotgun sequence.
ACCESSION   DASUEG010000310 DASUEG010000000
VERSION     DASUEG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250653"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFX11_05780"
     CDS             <1..426
                     /locus_tag="VFX11_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 domain-containing protein"
                     /protein_id="HEX4938163.1"
                     /translation="LITTTAEIGEGFRSNLYGLAVDLNLPGFAFFQFNYYVRNDVEFM
                     GGPAPNDTGSQITLVWLVPFDIGTTLWAFEGFLDYAYDVDQGEDNIIAGPRLLMDVGE
                     LFGEKGAVQAGVEYQIWRNKFSIDGIDEDVVQAMVKFIF"
     gene            457..1131
                     /locus_tag="VFX11_05785"
     CDS             457..1131
                     /locus_tag="VFX11_05785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX4938164.1"
                     /translation="MLNLQAQPAALSIDLARTALLLIDMQRDFLEPGGFGESLGNDVS
                     LLRDCIEPCRAVLAAARAQGLLVIHTREGHRPDLSDAPPAKINRGHPSVRIGAPGPMG
                     RILIRGEPGQDIIPELYPIAGEPVIDKPGKGSFYQTDLELILKNRNIDTLLVGGVTTE
                     VCVHTSVREANDRGFRCVVLADCCGSYFPEFHRVALQMVAAQGGIFGWVSDGEKLRQA
                     LQQAGA"
     gene            1171..2769
                     /locus_tag="VFX11_05790"
     CDS             1171..2769
                     /locus_tag="VFX11_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HEX4938165.1"
                     /translation="MNTTTFKPKIWSPGDWNALFGFGTNILVNMLVLTALLRFVLKLP
                     DEIVFGRILPACGLMLCLSTMYYAWLAYRLAQKTGRNDVCALPSGTSVPHMFVVVFVI
                     MLPISISTGDPIKGWEAGLTWVFVQSFVLMLGGYIAPYIRRITPRAALLGTLAGVSLA
                     FISMRPALEMYMTPVIGVICFAIILLSWFGGVRYFKGIPAGLVAIAVGTLIAWSSRAV
                     GLDYGGLSLEKLMGSFVNFGFAVPLPAFGHVFSGFEFLGIILITAIPFGIYDLVECMD
                     NVESAAVAGDEYPTTRVLTADGIISLIGCCMGNPFINAVYIGHPGWKSMGGRIGYSAA
                     TGVAVILLAWLGIISVVSSIIPLVAISPILLYIGMLIGAQAFQETPKSHAPAIILALT
                     PHLAAWGKLQIDSALGAAGTNAAAVGLDKLAQTGVLYEGLQVMAGGAIISGLVLGAIG
                     VFIIERQLLKAAAFALAGAVFTFFGFMHGETIGVMESPALAVAYLLVAVFMVGLHRFG
                     EASVPAAASVPEKAPEPALQGASA"
     gene            2799..>3310
                     /locus_tag="VFX11_05795"
     CDS             2799..>3310
                     /locus_tag="VFX11_05795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX4938166.1"
                     /translation="MGSRFVDSTPYRWPYNGDLRPDNTALIVIDMQTDFCGPGGYVDT
                     MGYDISLTRAPIEPIRQLLDSMRAKNFLIIHTREGHRPDLADLPANKRWRSRQIGAGI
                     GDPGPCGNILIRGEPGWDIIPELAPLPDEIIIDKPGKGSFCATDLELILRLKGIDNIV
                     LTGITTDVCV"
BASE COUNT          606 a          933 c         1079 g          692 t
ORIGIN      
        1 ctgatcacca ccaccgcgga aatcggtgaa ggtttccgca gcaatctgta cggtctggcg
       61 gtggacctca atctgccggg tttcgcattc ttccagttca actactacgt acgcaacgac
      121 gtggaattca tgggcggccc ggcaccgaac gataccggtt cgcagatcac gctggtgtgg
      181 ctggtgccgt tcgacatcgg caccaccctg tgggcgtttg aaggctttct cgattacgcc
      241 tacgacgtcg atcagggcga ggacaacatc atcgccgggc cgcgtctgct gatggatgtg
      301 ggcgaactgt tcggcgaaaa gggcgccgtg caggcgggcg tggaatacca gatctggcgc
      361 aacaagttca gcatcgacgg catcgatgag gacgtggtgc aggcgatggt gaaattcatt
      421 ttctgaatgt tgacgaccaa caggaggatg gccccgatgc tgaatctgca ggcacaaccg
      481 gcggcactgt ccatcgatct ggcacgcact gcgctgctgc tgatcgacat gcagcgggat
      541 tttctggaac ccggtggctt cggtgaatcc ctcggcaacg atgtgtcgtt gctgcgggac
      601 tgcatcgaac cctgccgcgc cgtgctggcg gcggcccggg cccagggtct gctggtgatt
      661 cacacccgtg aaggtcaccg gccggatctg agcgacgcac cgccggccaa aatcaatcgc
      721 ggccatccgt ctgtgcgcat cggtgcgccc gggcccatgg gtcgcatcct gattcgcggt
      781 gaaccggggc aggacattat tccggagctg tatccgattg ccggcgaacc ggtcatcgac
      841 aagccgggca agggttcctt ctaccagacg gatctggagc tgatcctgaa aaaccgcaat
      901 atcgacacgc tgctggtggg cggtgtcacc acggaagtgt gcgtgcacac cagcgtgcgg
      961 gaagccaatg accgtggttt tcgctgtgtg gtgctggcgg attgttgcgg gtcgtatttt
     1021 ccggaattcc accgggttgc gctgcagatg gtagcggcgc agggcggcat tttcggctgg
     1081 gtttcggatg gggaaaaact gaggcaggcg ttacaacagg cgggcgctta aaccaagcgg
     1141 gctctcacat aagcaagggg gtaacaacag atgaacacga ccacattcaa accgaaaatc
     1201 tggtcgcctg gcgactggaa cgcgttgttc ggcttcggca cgaacattct ggtgaacatg
     1261 ctggtgttga cggcattgtt gcgctttgtg ctgaaattgc cggacgaaat cgtcttcggt
     1321 cgcatcctgc ctgcgtgcgg gctgatgctg tgcctgagta cgatgtacta cgcctggctg
     1381 gcgtaccggc tggcgcagaa aaccgggcgc aacgatgtgt gcgcgctgcc atcgggcacc
     1441 agtgtgccgc acatgttcgt ggtggtgttc gtgatcatgc tgccgatcag catcagcacc
     1501 ggcgatccca tcaagggctg ggaagcaggg ctgacctggg tgttcgtgca gagcttcgtg
     1561 ctgatgctgg gcggctatat tgcaccttac atccgcagaa ttactccgcg cgcggcgctg
     1621 ctgggcacgc tggccggtgt gtcgctggcg tttatttcca tgcggccggc gctggaaatg
     1681 tacatgacgc cggtgatcgg cgtgatctgc ttcgccatca ttctgctgag ctggtttggc
     1741 ggcgtgcgct atttcaaggg cattccggca ggactggtgg cgattgcggt aggcacactg
     1801 atcgcctgga gttcgcgtgc ggtgggactg gattacggcg gcttgtcgct ggaaaaactg
     1861 atgggatcgt ttgtgaattt cggttttgcg gtgccgctgc cggcgttcgg tcacgtgttt
     1921 tccggttttg aatttctcgg catcatcctg atcaccgcca ttccgttcgg catttacgat
     1981 ctggtggaat gcatggacaa cgtggaaagc gcagcggtgg cgggcgatga atatcccacc
     2041 acgcgggtgc tgacggcgga cggcatcatc agcctgatcg ggtgctgcat gggcaatccg
     2101 ttcatcaacg cggtgtacat cggtcatccg ggctggaaat ccatgggcgg ccgcatcggc
     2161 tattccgctg ccacgggcgt ggcggtgatt ctgctggcgt ggctcggcat catttccgtg
     2221 gtgtcgtcga tcattccgct ggtggcgatc tcgccgatat tgttatacat cggcatgctg
     2281 atcggtgcgc aggcgtttca ggaaacgccg aagtcgcatg caccggcgat cattctcgca
     2341 ctcacgccgc acctggcggc ctggggcaaa ctgcagatcg acagcgcact gggtgcagcc
     2401 ggcaccaacg cagcggcggt gggattggac aaactggcgc aaaccggtgt gctgtacgaa
     2461 ggcctgcaag tcatggcggg cggcgcgatc atcagcggtc tggtgctggg cgccatcggc
     2521 gtgttcatca tcgagcggca gttgctgaaa gcggcggctt tcgccctggc tggcgcggtg
     2581 ttcaccttct tcggtttcat gcacggcgaa accatcggtg tgatggaatc accggcactg
     2641 gcggttgcct atctgctggt ggcggtattc atggtcggcc tgcaccgttt cggcgaagcc
     2701 agtgtgccag cggccgcaag tgtaccggaa aaagcgccgg agccggcgtt gcaaggcgca
     2761 tcggcctgaa ccggatcgac agtggaggag aggacatcat gggatcacgc ttcgtcgatt
     2821 cgacacccta ccgctggccc tacaatggcg atctgcgacc ggacaacacg gcgctgatcg
     2881 tcatcgacat gcagacggat ttctgcggcc ccggcggcta cgtggacacg atgggttacg
     2941 atatttcgct gacccgcgcg cccatcgagc cgatccggca gttgctggac agcatgcggg
     3001 cgaaaaattt cctgatcatt cacacccgcg aaggccaccg gcccgatctg gcggacctgc
     3061 cggcgaacaa gcgctggcgg tcacgacaga tcggtgccgg tatcggcgat cccggcccct
     3121 gcggcaatat cctgatccgt ggtgagccgg gctgggacat cattccggaa ctggcaccgc
     3181 tgcccgatga aatcatcatc gacaaaccgg gcaagggttc gttctgcgcc accgatctgg
     3241 aactgatcct gcggctgaag ggcatcgaca atatcgtgct caccggcatc accaccgacg
     3301 tgtgcgtgca
//
LOCUS       DASUEG010000311         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1019522, whole genome shotgun sequence.
ACCESSION   DASUEG010000311 DASUEG010000000
VERSION     DASUEG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019522"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFX11_05800"
     CDS             complement(<1..1463)
                     /locus_tag="VFX11_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4938167.1"
                     /translation="MIRSRAAVAGWRGVVVSGLICVGVQSAQAEDVAVTQAATPELEA
                     VVVTASRTATGLGETPAAISRLENDAITDSKPRLISDVIDRVPGVHMVDLGNEQHSMS
                     IRQPITTSALYQYLEDNIPIRPLGVFNHNALNEINLAGVDSVEVMRGPTSSLYGSNAV
                     GGAVNFLSAAPSLTPEAWAGLRGSDQGYGRVDVGASGTEGDLGARLSYYRSVVNDGWR
                     EYGDGDKDSLSARVDYVLSDSSLVKTVGSYTNLYTDMSGSLGESDYQNNPEKSYQTFT
                     ERTDINSRISSSLETEWDADTSTRATLFWRDNSHGQIPSYQLGSCQPTPPAETCTTNG
                     RQNDNSYTSYGFDSQFNRALGFMDARMILGAGWDRTKNDYSEDNLSVLRDTELRYVSY
                     TVTSKRRRYDALIDNPSVYFQGEIKPLDLTTVVLGGRYDKIRYDFDNHLAPSSSTGAP
                     DEIREFSHVSPRLGIARELGQDAELFLNLSQGFVPPEV"
     gene            complement(1577..2032)
                     /locus_tag="VFX11_05805"
     CDS             complement(1577..2032)
                     /locus_tag="VFX11_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938168.1"
                     /translation="MRFLCFKHERIYDQQPDEHLMEQWNAWMHSAGVCYDCGDWSKAA
                     SFVGSAFDLAKIRLAKNAQVRADAMAQVTLSAIYLANVLQHMGHLCESRYALQMAGDC
                     LNCFSCTEDAACASELLSCLEDENLREQLIGRFLNLPYRPCRWSRRGLH"
BASE COUNT          489 a          747 c          654 g          494 t
ORIGIN      
        1 acctccggcg gcacaaaacc ctggctcagg ttgagaaaca gctcggcatc ctggcccagt
       61 tcgcgcgcaa taccaagacg cggcgacaca tgggagaatt cgcgaatctc gtccggcgcg
      121 ccggtggagg atgacggcgc cagatgattg tcgaagtcgt agcggatttt gtcgtagcga
      181 ccacccagca ccacggtggt caggtccagc ggttttattt caccctgaaa atacacggat
      241 ggattgtcga tcagcgcatc gtagcgccgg cgtttgctgg tcaccgtata ggaaacgtaa
      301 cgcaactccg tatcgcgcag caccgacaga ttatcctcgc tgtaatcatt tttcgtgcga
      361 tcccagccgg cacccagaat catccgcgca tccatgaagc ccagcgcgcg attgaactgc
      421 gaatcgaaac cgtaagaagt gtaggaattg tcgttctggc ggccgttggt ggtgcaggtc
      481 tccgccggcg gcgtgggctg gcaactgccc agctggtaac tgggaatctg gccgtgggaa
      541 ttatcccgcc agaacagcgt cgcgcgggtg ctggtgtccg catcccactc ggtttccagg
      601 ctgctgctga tgcgcgaatt gatatcggtg cgttcggtaa atgtctggta acttttttcc
      661 ggattgttct ggtaatcgct ctcgcccagt gaaccgctca tgtcggtgta caggttggtg
      721 tagctgccca cggttttcac cagcgaagaa tccgacagca cataatccac ccgcgcgctc
      781 agcgaatcct tgtcgccgtc gccgtactcg cgccagccgt cattcactac actgcgataa
      841 taggaaagcc gcgcgcccag atcaccttcc gtgccactgg cgcccacatc cacccggcca
      901 taaccctgat cgctgccgcg cagtccggcc caggcttccg gcgtcaggct gggcgcagcc
      961 gacagaaaat tcaccgcgcc acccaccgca ttgctgccat acagcgacga cgtggggccg
     1021 cgcatcactt ccacgctgtc cacaccggca agattgattt cgttgagcgc gttgtgattg
     1081 aacacgccca gcggacgaat cggaatattg tcttccagat actgatacag ggcgctggtg
     1141 gtaatgggtt ggcggatgct catgctgtgc tgttcgttgc ccagatccac catgtgcacg
     1201 ccgggcactc ggtcgatcac atcgctgatc aggcgcggct tgctgtcggt gatggcatca
     1261 ttttccagcc gggagatggc cgccggcgtt tcgcccagac cggtggcggt gcgcgaagcc
     1321 gtcaccacca cggcttccag ttccggcgtt gctgcctgtg tcaccgcaac gtcctctgcc
     1381 tgagcggact gcacacccac gcagatcaaa ccactcacca caactccacg ccatcccgcc
     1441 actgcggcgc gactccgaat cattgccatc ctgcccgtcc ccactgcaaa acgaaaggca
     1501 ggatcttaat gattcaaaca cttgcgcggg atgtggcata ttgtcgcacc cggtcccatt
     1561 ggccgcgcgg caacgctcag tgcaatcccc ggcgcgacca ccggcacggg cgatacggca
     1621 gattcaaaaa gcgtccgatc aattgctcgc gcaggttttc atcttccaga cacgacaaca
     1681 attccgacgc acaggccgca tcctcggtgc aggaaaagca gttcaggcag tcccctgcca
     1741 tttgcagcgc atagcgcgat tcgcacagat gccccatgtg ctgcagcacg ttggccaggt
     1801 aaatcgccga caaggtgacc tgcgccatgg cgtcggcgcg cacctgggca ttcttcgcca
     1861 gccggatctt ggccagatcg aatgcgctgc caacgaacga cgccgccttg ctccagtcgc
     1921 cgcagtcata gcaaacgccg gcggaatgca tccaggcatt ccactgttcc atcaggtgtt
     1981 catccggttg ctggtcgtaa atccgttcgt gcttgaagca gagaaatcgc atgggaacct
     2041 ccttggctgg attcttttaa atgataatga ttatcattta aaatgcaacc gcgagttatc
     2101 tgcttttttc agccggatcg ttttcgacct tcattgatga cggccctcca tttgccctgc
     2161 cctcaaatac acgcacaccc accaatgcct gaagggtgaa aaggacaggg ttgtgtggta
     2221 gcgttttgcc gcgttcagcc cccccgcttt tgaatcaagg aaaccatcca tggcaacaca
     2281 gcagctggca gcactcgcgg aatcgaccgg tgtcaaattt ggcacgagcg gcgtgagggg
     2341 gctggttgcc gccatgaccg accgcgtctg ctatgcctac accg
//
LOCUS       DASUEG010000312         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1108149, whole genome shotgun sequence.
ACCESSION   DASUEG010000312 DASUEG010000000
VERSION     DASUEG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108149"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VFX11_05810"
     CDS             complement(<1..160)
                     /locus_tag="VFX11_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4938169.1"
                     /translation="MARKTPLSRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVH
                     DGAATMDWM"
     gene            complement(206..676)
                     /gene="rpsG"
                     /locus_tag="VFX11_05815"
     CDS             complement(206..676)
                     /gene="rpsG"
                     /locus_tag="VFX11_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX4938170.1"
                     /translation="MPRRRVAAKREILPDPKFQSQILAKFINHVMESGKKSVAERIVY
                     GALDKVGERSKQNPVEIFEKALDALRPAVEVKSRRVGGATYQVPVEVRPSRRTALAMR
                     WMVDAARNRGEKSMPLRLAGEIMDAVEGKGAAVKKREDVHRMAEANKAFSHYRF"
     gene            complement(775..1149)
                     /gene="rpsL"
                     /locus_tag="VFX11_05820"
     CDS             complement(775..1149)
                     /gene="rpsL"
                     /locus_tag="VFX11_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX4938171.1"
                     /translation="MATINQLVRKPRERRAAKSDVPALQSCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGYEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGTL
                     DTAGVNGRMQARSKYGTKRPKK"
     gene            complement(1294..>4542)
                     /gene="rpoC"
                     /locus_tag="VFX11_05825"
     CDS             complement(1294..>4542)
                     /gene="rpoC"
                     /locus_tag="VFX11_05825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4938172.1"
                     /translation="KRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLH
                     ECGLPKKMALELFKPFIFAKLEARGLATTIKAAKKMVERETAEVWDILAEVIREHPVL
                     LNRAPTLHRLGIQAFEPQLIEGKAIQLHPLVCVAYNADFDGDQMAVHVPLTLEAQLES
                     RALMMSTNNILSPANGEPIIVPTQDVVLGLYYISRERVNAPGEGMTFVDTKEVSRALA
                     ARSVTVHSRVKVRVTETVKQEDGSLVERTFIASTTAGRCMLWDVLPKGLPFELINQPM
                     TKKTVSRLINACYRRLGLKATVIFADKLMYLGFHQATLSGSSIGIEDMRIPEEKARII
                     SEAEDEVREIEDQFASGLVTQGERYNKVVDIWSRTNDMVAKAMMEHLSTEKVKDKDGK
                     IVDQKSFNSIFIMADSGARGSQAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVL
                     QYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEEDCGTTNGVIMTPLIEG
                     GDVVEPLGERVLGRTVAQDVLKPGTDDVLAPAGTLIDEKWVERLEAEGVDEIVCRSPI
                     ACDTRYGVCRKCYGRDLARGHLVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRS
                     SAVSNVQVKTHGTIRLHNLKTVEQTNGNLVAVSRSGELAVADDKGRERERYKLPYGAV
                     IQVKDGSKVEPGQVVAQWDPHTHPVITELAGRITFGEFEEGITVKQTTDDLTGLTNYE
                     IIDPKDRPSSGKDKRPVVRLVNEKNELVTMPGSDTPVQYYLPAGSIVMLENGVKVEAG
                     DVVARIPQESSKTRDITGGLPRVADLFEARKPKESSILAEKSGVVSFGKETKGKVRLM
                     ITPDDGSDAYEELIPKWRQLNIFEGERVEQGEVISDGPSNPHDILRLKGVNAVASYIV
                     NEVQDVYRLQGVKINDKHIEVIVRQMLRKVNISEPNDSSFIKGEQLELARVLDENERL
                     DKDGKFPAKFDRELLGITKASLSTESFISAASFQETTRVLTEAAVTGKVDDLRGLKEN
                     VVVGRLIPAGTGFAYHAERRRARHADDNEQVPQFTADDAEQALSAALSSPGE"
BASE COUNT          849 a         1358 c         1311 g         1024 t
ORIGIN      
        1 ccatccagtc catggtggca gcgccgtcat gcacttcacc gatcttgtga gacacgccgg
       61 tatagaacag gacgcgctcg gtggtagtgg ttttgcccgc atcgatgtgc gcgctgatac
      121 caatgttacg gtatctggaa aggggtgttt tacgtgccac aggcttaccc tcggactcgc
      181 taaatgattg ataaaggtga caaaattaga agcggtagtg agagaaagcc ttgttggctt
      241 ctgccatccg gtgaacgtct tcacgcttct tcacggctgc acccttacct tcaacggcat
      301 ccatgatttc acccgccagg cgcagcggca tggatttttc gccacggtta cgggccgcat
      361 ctaccatcca gcgcatggcc agggcggtac gacggctggg acgcacttca acgggcacct
      421 ggtaggtagc accaccaacg cggcgggact ttacttccac ggcgggacgc agtgcgtcca
      481 gggctttttc aaagatttca acggggtttt gcttgctgcg ctcgccaacc ttgtccagcg
      541 cgccgtatac gatgcgctcg gcaacggatt tctttccgct ttccatgaca tggttgatga
      601 acttggccag aatctgactc tggaacttcg gatcgggcag aatttcgcgt ttcgcggcaa
      661 ctcgtcttct tggcatgact ctattcctgt ttcaggtggt tccaagactg tgcttggcct
      721 tactcccgcg ctagggtgcg ggatcaaaat gacagccggt ttaccggcca ccgattattt
      781 cttggggcgc ttggtgccgt atttggagcg cgcttgcata cggccgttca cgccggcagt
      841 atccagggtt ccacggacgg tgtgataacg cacacccggc aggtctttta cacgaccgcc
      901 acggatcagg attacagagt gctcttgcag gttgtggcct tcgccgccga tgtaagaagt
      961 cacttcgtaa ccgttggtca aacgtacacg tgctactttc cgcagagccg agttaggctt
     1021 cttgggagta gtggtgtaca cgcgggtgca tacgccgcga cgctgcgggc agctttgcaa
     1081 cgcaggaacg tcgctcttgg ctgctcggcg ctcacgtggc ttacgcacca attggttaat
     1141 ggttgccatg tagctatttc tccagttcta ccgataaaaa cgcaaaatcc cgtcaccccc
     1201 tttcggggat gcgagattct aggggtgacc cgccagcaag tcaagcgagc cggcgggctc
     1261 tcccggaccg ggaaagcccg ccgtttccgg cagttattcg ccgggtgaac tcaatgccgc
     1321 gctcagggcc tgctcggcat cgtccgcggt aaattgcgga acctgttcat tatcatcagc
     1381 atggcgggcg cgacggcgtt ccgcatggta cgcaaaaccg gtacccgcgg gaatcagacg
     1441 tcccactacc acgttttcct tcagaccgcg caggtcgtcc accttgccgg tgaccgcagc
     1501 ttcggtcagt acccgggtgg tttcctggaa cgatgcggcg gagatgaacg actcggtcga
     1561 cagcgatgcc ttggtgatac ccagcagttc gcgatcgaac ttggccggga acttgccgtc
     1621 cttgtccaga cgctcgttct cgtccaggac ccgcgccagc tccagttgct cacccttgat
     1681 aaaggaagag tcgttgggtt cgctgatgtt caccttacgc agcatctgcc gcacgataac
     1741 ttcgatgtgc ttgtcgttga tctttacgcc ctgcagacgg taaacgtcct gcacctcgtt
     1801 gacgatgtag ctcgccacgg cattcacgcc tttcaggcgc aggatgtcgt gagggttgga
     1861 cgggccgtcg gaaatgactt cgccctgctc cacccgctcg ccttcgaaga tgttgagctg
     1921 acgccacttg ggaatcagtt cttcataggc gtcgctgccg tcgtcaggcg taatcatcag
     1981 acgcactttg cctttggtct ccttgccgaa ggacactacg ccgctcttct ccgccaggat
     2041 ggaggattcc ttcggcttgc gcgcttcgaa caggtcggca acgcgcggca gaccaccggt
     2101 gatgtcccga gtcttcgagc tttcctgcgg aatacgcgcg accacatcgc ccgcttccac
     2161 cttgacgccg ttttccagca tgacgataga cccggccggc aggtagtact gcaccggcgt
     2221 atcgctgcca ggcatggtga ccagctcgtt cttctcgttg accagacgca ctaccggacg
     2281 cttgtccttg ccggacgacg gacggtcttt cggatcgatg atttcgtagt tggtcagacc
     2341 ggtcagatcg tcggtggtct gcttgacggt aatcccttct tcgaattcac cgaaggtgat
     2401 ccggcccgcc agttcagtga taaccgggtg agtgtgcgga tcccactggg caacaacctg
     2461 gccaggctca accttgctgc cgtccttcac ctggatgaca gcaccataag gcagcttgta
     2521 gcgttcacgc tcgcgccctt tgtcgtcggc taccgccagt tcgccggagc gggaaaccgc
     2581 taccaggttg ccattggtct gttccaccgt cttcaggttg tgcaaacgga tggtaccgtg
     2641 tgttttcacc tggacgttgc tgaccgccga gcttctggag gccgcaccac cgatgtggaa
     2701 cgtacgcatg gtcagctggg taccgggctc accgatagac tgggctgcaa taacgccgac
     2761 ggcttcaccg atgttcacca gatggccacg ggccaggtca cgaccgtagc acttgcggca
     2821 aacgccgtag cgggtgtcgc aggcaatcgg cgaacggcag acgatttcat ccacaccttc
     2881 cgcttccaga cgctcaaccc acttctcatc gatcagggtg ccggccggtg ccagtacgtc
     2941 atcggtgcca ggcttcaata cgtcctgagc cacagtgcga cccagtacgc gctcacccag
     3001 cggctcaacc acgtcgccgc cttcaatcag cggcgtcatg ataacgccgt tggtggtgcc
     3061 acaatcttct tcagtaacta ccaggtcctg ggccacgtcc accagacggc gggtcaggta
     3121 gccggagtta gcggttttca gtgcagtatc cgccagacct ttacgagcac cgtgggtcga
     3181 gatgaagtac tggagtacgt tcagaccctc acggaagttc gccgtgatcg gcgtttcgat
     3241 gatggagccg tcgggcttgg ccatcagacc ccgcataccc gccagctgac ggatctgggc
     3301 ctgggagccc cgcgctccgg agtccgccat gatgaagatg gagttgaaag acttctggtc
     3361 gacgatcttg ccgtccttgt ccttcacctt ctcggtggag aggtgctcca tcatcgcctt
     3421 ggcgaccatg tcgttggtgc gggaccagat gtcgaccact ttgttgtagc gctcgccctg
     3481 ggttaccaga ccggaggcaa actggtcttc gatctcgcgc acttcatctt ctgcttccga
     3541 gatgatgcgg gccttctctt ccggaatccg catgtcttcg ataccgatcg aggaaccgga
     3601 cagcgtggct tgatggaagc ccaggtacat cagtttgtcg gcgaagatta cggtcgcttt
     3661 cagacccagc cgccggtagc aggcgttgat cagacggctg acagtcttct tggtcattgg
     3721 ctggttgatc agctcgaacg gcagaccttt gggcagcaca tcccacaaca tgcagcgacc
     3781 ggccgtagtg ctcgcaatga aggtacgctc aaccagcgag ccatcttcct gcttcaccgt
     3841 ttcggtgaca cgcaccttga cacgggaatg cacggtgacc gacctggcgg ccagggcacg
     3901 ggagacttcc ttggtgtcga caaaggtcat gccttcgcct ggcgcattaa cgcgttcacg
     3961 gctgatatag tacagaccca gtacaacgtc ctgggtaggc acgatgatgg gttcaccatt
     4021 cgccggggac aggatgttgt tggtggacat catcagcgcg cgggattcga gctgggcttc
     4081 cagggtcagc ggtacgtgta ccgccatctg gtcaccgtcg aagtcggcgt tataggccac
     4141 gcacaccagc gggtgcagct ggatcgcctt gccttcgatc agttgcggtt cgaacgcctg
     4201 gataccgaga cggtgcagcg tgggcgcacg gttcagcagt accgggtgtt cgcggatgac
     4261 ttcggccagg atgtcccaca cttctgctgt ttcacgctcg accattttct tcgcggcttt
     4321 aatcgtggtc gcgagaccac gggcttccag cttggcaaag atgaaaggct tgaacagttc
     4381 cagcgccatc ttcttgggca gaccgcactc gtgcagcttc agggtcggac ctaccacgat
     4441 taccgaacgg ccagagtagt cgacgcgctt gcccagcagg ttctgacgga agcgaccctg
     4501 cttgcccttg atcatgtcag ccagggattt cagcgggcgc tt
//
LOCUS       DASUEG010000313         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1773346, whole genome shotgun sequence.
ACCESSION   DASUEG010000313 DASUEG010000000
VERSION     DASUEG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773346"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFX11_05830"
     CDS             complement(<1..498)
                     /locus_tag="VFX11_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4938173.1"
                     /translation="MSNVTFFTSTAGVAVPRILYGTAWKKERTTALVLQAFRAGFRGI
                     DTACQPKHYDEAGVGGALRQLQTQGIRRGDYYLQTKFTPLSGQDPERVPYDPSAPLAD
                     QVAQSFAASCRNLHTDQLDGLILHSPLPTAAQTLTAWRAMEQIQQQGGTRQLGISNCY
                     DLRVLQ"
     gene            579..1424
                     /locus_tag="VFX11_05835"
     CDS             579..1424
                     /locus_tag="VFX11_05835"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX4938174.1"
                     /translation="MAYTTLIQPEDLIPNLHQPDWRIIDCRHDLAQPDAGENAYRQSH
                     LPGALFLHLDRDLSGAKTGTNGRHPLPEPAALAITLGRCGIGPGTQVVAYDDSGGMFA
                     ARLWWLLRWLGHEAVAVLDGGLAAWQRAGQALSAVSEEYPPALFNGQPALLTLTADDI
                     LRQLDEPRWQLLDARAPDRFRGENESIDPVAGHIPGALNRFFRDNLDSDGRFKPAEKL
                     KKEFSIVLGELEPEQVVHQCGSGVTACHNLLAMELAGLAGSRLYPGSWSEWCSDPARP
                     VARGV"
     gene            complement(1434..1679)
                     /locus_tag="VFX11_05840"
     CDS             complement(1434..1679)
                     /locus_tag="VFX11_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938175.1"
                     /translation="MLSCRDMAHEATDMMEGRVTWQRRLALWWHLFLCVHCRRFIRHL
                     KIQRRLSGLRPTPPADPATVKRVMDRVKDRVSKDSTE"
     gene            complement(1673..2296)
                     /locus_tag="VFX11_05845"
     CDS             complement(1673..2296)
                     /locus_tag="VFX11_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4938176.1"
                     /translation="MTEPTPFLSDQHIAQLRRRDHRAFETLVSQLHRPLVAVARSIIG
                     ESLAEEVVQEAWVSIYRALPEFEGRSSLKTWIYTIVGNEARTRLRKEKRIVALEDLAL
                     PGTDYLDSSRFKADGHWDTPPPDWHLESPEQLLEEAQLQNCIEVTLDQLSPNQKAVFT
                     LRDLEQQSLDDICNILQLSDSNIRVLLHRARLRLMQVIDRYQVTGEC"
     gene            complement(2289..2762)
                     /locus_tag="VFX11_05850"
     CDS             complement(2289..2762)
                     /locus_tag="VFX11_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938177.1"
                     /translation="MKAFAHRLVLFWAPTLQAGVMLSSLLLTGCASPPVLPDLQGQAA
                     VVMGFTTEEAKPYEGGTVYTGLLIHRINGASVGETPLRRDDYRTVPAGKVVVEGQCYW
                     YWRGISWETHQDLLEPARLEWDAQPGHVYTLFVTIDEYKATCHLELFDKLQTTHD"
     gene            complement(2767..>3267)
                     /locus_tag="VFX11_05855"
     CDS             complement(2767..>3267)
                     /locus_tag="VFX11_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX4938178.1"
                     /translation="FGRKYPAAQVIAATHRNERTESYWLAYTEEQIEATFALCIALCA
                     TYPVWEILGHEEIAPGRKDDPGPAFPLDRLRQRLIARGRDEDTGFNRKPDGDIDPAAP
                     ARGKGFVTASQLNFRAEPRASADAVAPPLPRGTVVEILDQKAGWHQVRVNGKIGWLKS
                     EYLKTG"
BASE COUNT          541 a          985 c         1084 g          657 t
ORIGIN      
        1 ttgcagcacg cgcagatcat aacaattgct gatgcccagc tggcgcgtgc cgccctgctg
       61 ctggatctgc tccattgcgc gccaggcggt gagcgtttgc gccgctgttg gaagtggtga
      121 atgcaggatc aggccgtcca gttgatccgt gtgcaggttg cggcaggacg cggcaaagga
      181 ttgcgccacc tgatccgcca gtggcgcgct gggatcatag ggcacccgct cggggtcctg
      241 gccggacagc ggtgtgaatt tcgtttgcag ataataatcg ccgcgccgga tgccctgggt
      301 ttgcagttgg cgcagggcgc cgcccacgcc ggcttcgtca taatgtttgg gctggcaggc
      361 agtgtcgatg ccgcgaaagc cggcgcgaaa tgcctgcaac accagcgcgg tggtgcgttc
      421 ctttttccag gcggtgccgt acaggatgcg tggcacggcg acgccggcgg tactggtgaa
      481 aaaggtgacg ttcgacatgg ggctgtcctg cgggttcgat tgtcggaaat tctgtgtcat
      541 cctgcgcgta cagcaaatta tgcaccggac cccatgccat ggcctatacc accctgatcc
      601 agccggaaga cctgattccc aatctgcacc agcccgactg gcgcatcatc gattgccggc
      661 atgatctggc gcagccggat gcgggtgaaa acgcttaccg tcagagccat cttcccggcg
      721 cgctgttcct gcatctggat cgcgatcttt ccggcgcgaa aaccggcacc aatgggcgcc
      781 atccgctgcc ggagcctgcc gcgctcgcca tcaccctggg ccgctgtggc attggtccgg
      841 gcacccaggt ggtggcctat gacgacagtg gcggcatgtt cgcggcgcgc ctgtggtggc
      901 tgctgcgctg gctgggccac gaagcggtgg cggtgctgga tggcgggctg gccgcctggc
      961 agcgggccgg gcaggcgctg agcgctgtca gcgaagaata tccgccggcg ctgttcaacg
     1021 ggcagccggc gttgctcacg ctgacggcgg acgacatcct gcggcagctg gacgaaccgc
     1081 gctggcagtt gctggatgcg cgggcgccgg accgctttcg tggcgaaaat gaatcgattg
     1141 atccggtggc gggccatatt cccggtgcgc tgaaccgctt ttttcgcgac aacctggaca
     1201 gcgacggccg cttcaagcca gcggaaaaat tgaagaagga attcagcata gtgctgggag
     1261 agctggaacc ggagcaggtg gtgcatcagt gcggttccgg tgtcaccgct tgccacaatc
     1321 tgctggcgat ggaacttgcg ggccttgcgg gttcgcgcct gtatcccggg tcgtggagtg
     1381 aatggtgcag cgatccggcc cggccggtgg cgcggggcgt ttgaagcgct tctctattca
     1441 gtggaatcct ttgacacgcg gtctttcacc cggtccatca cccgtttcac tgtggcagga
     1501 tcagccggtg gcgtcggccg caggccgctc aggcgccgct ggattttcag atggcggatg
     1561 aagcggcggc aatgcacgca gaggaacagg tgccaccata acgccagccg gcgttgccag
     1621 gtcacccgcc cttccatcat gtcggtggcc tcgtgggcca tgtcgcggca gctcagcatt
     1681 ctccggtcac ctgatatcga tcaatcactt gcatcagtcg aaggcgcgcc cgatgcagca
     1741 gcacccgaat attggagtcg ctcagctgca ggatgttaca gatgtcgtcc agactttgct
     1801 gttccagatc gcgcagggtg aacacggcct tctggttggg gctgagctgg tccagggtga
     1861 cttcgatgca gttctgcagc tgggcttctt ccagcaattg ttccggcgat tccagatgcc
     1921 agtccggcgg cggcgtgtcc cagtggccgt cggccttgaa gcggctgctg tccaggtaat
     1981 cggtgccggg tagtgccaga tcctccagcg ccacgatgcg tttttccttg cgcaggcggg
     2041 tgcgggcttc gttgccgaca atggtgtaaa tccaggtttt cagcgacgag cgcccctcga
     2101 attccggcag cgcgcggtag atggacaccc aggcttcctg caccacctcc tcggccagac
     2161 tttcgccgat gatggaacgg gccaccgcta ccagcggccg gtgcagttgc gacaccagcg
     2221 tttcgaaggc gcggtgatcg cggcggcgca gttgtgcgat gtgctggtca gacaaaaacg
     2281 gcgtgggttc agtcatgggt ggtttgcagc ttgtcgaaca gttccagatg gcaggtggcc
     2341 ttgtattcgt cgatggtcac gaacagggtg tagacatgcc ctggctgtgc gtcccattcc
     2401 agccgggcgg gttccagcag gtcctggtgc gtttcccatg agatgccgcg ccagtaccag
     2461 tagcactgac cttccacaac gactttgccc gctggcaccg tgcgatagtc atcacggcgc
     2521 agtggcgtct cacccacact ggcgccgttg atgcggtgaa tcagcaggcc ggtgtaaacg
     2581 gtgccgcctt cgtagggttt ggcttcctcg gtggtgaagc ccatgaccac agcggcctgg
     2641 ccttgcaggt ccggcagcac cggcgggctg gcgcaacccg tcagcagcag gctgctcagc
     2701 atcacgccgg cctgcaacgt cggtgcccaa aataaaacaa ggcgatgcgc gaatgctttc
     2761 aagccgtcat cccgttttca gatattccga tttcagccag ccgatcttgc cgttgacccg
     2821 cacctgatgc cagccggctt tctggtccag tatctccacc acggtaccgc gcggcaacgg
     2881 tggcgcgacg gcatcggcgc tagcgcgggg ttcggcgcgg aaattcagct ggctggcggt
     2941 gacgaaaccc ttgccccggg ccggcgcggc gggatcgatg tcgccgtcgg gcttgcggtt
     3001 gaagccggtg tcttcatcgc ggccccgtgc aatcagccgt tgccttaggc ggtccagcgg
     3061 aaacgccggg cccggatcat ccttgcggcc cggcgcgatt tcttcgtggc ccagaatttc
     3121 ccacaccggg taggtggcgc agagggcaat gcacagggca aaggtggctt cgatctgttc
     3181 ttcggtgtag gccagccagt agctttccgt gcgctcattg cggtgtgtgg cggcgatcac
     3241 ctgtgccgcc ggatacttgc ggccgaa
//
LOCUS       DASUEG010000314         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_693293, whole genome shotgun sequence.
ACCESSION   DASUEG010000314 DASUEG010000000
VERSION     DASUEG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693293"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFX11_05860"
     CDS             <1..495
                     /locus_tag="VFX11_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4938179.1"
                     /translation="SVEFFMNVHAGWLATTVLTAVLSFSTSLAQAEVYRWVDANGRVH
                     FGDRAPGDRKAEALDMPEATGGVVTQDVSDQELRERQERVSRVLEEERLEKEQQKEAL
                     KQQEAEKKVYCERFRNRMERLEASSQVYSENKDGTVKYWKDKDADRYKAKQREQYQAE
                     CGTS"
     gene            complement(543..1403)
                     /gene="speE"
                     /locus_tag="VFX11_05865"
     CDS             complement(543..1403)
                     /gene="speE"
                     /locus_tag="VFX11_05865"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX4938180.1"
                     /translation="MKSSQENWFTEVFGNEGTAFSLAVTEKLHEEQTRYQYLEVYQTR
                     TFGKLMVLDGCVMLTERDNFLYHEMMSHPALFTHPEPKHVAIIGGGDCGTLREVLKHP
                     GIEKCTQIDIDERVTWAAEKWFPELCDANHDPRAELLFDDGIAWVRNAAPGSLDVIII
                     DSTDPVGPAEGLFAVDFYKDCRKALKPEGILVQQSESPLLHADTIIKKIHQDMKTAGF
                     QQCHTLPFPQPVYPTGWWSCTMASPQGSVTRFRDDESAAKTFATRYYNVDIHRGALAV
                     PEFMKVALGK"
     gene            1550..>2220
                     /locus_tag="VFX11_05870"
     CDS             1550..>2220
                     /locus_tag="VFX11_05870"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505852.1"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX4938181.1"
                     /translation="MATPPIEAARDTYSIDNWSDGYFDINGEGQIDVGTGPDVGRVAL
                     SAVVDAARVAGLRTPLLIRFTDILHDRVTKLSGAFAGAMQQLDYDADYTAVYPIKVNQ
                     QRRVVEQIYGTAAGHPRCSVGLEAGSKPELMAVLALSRAGSVVVCNGYKDREFVRLAL
                     IGEKMGRRVYIVVEKMTELALVLKEAAALNVQPRIGLRTRLSSIGKGNWQNTGGEKSK
                     FGLSAT"
BASE COUNT          444 a          611 c          734 g          431 t
ORIGIN      
        1 tctgtggagt ttttcatgaa tgtgcatgca ggctggctgg caacgactgt gctgacagca
       61 gtgttaagtt tttcgacatc tctggcccag gccgaggtct accgctgggt ggatgccaat
      121 ggcagggttc atttcggtga ccgggccccg ggggaccgaa aggctgaagc cctggacatg
      181 ccggaggcta ccgggggagt ggtaacccag gatgtcagcg accaggaact ccgcgaacgt
      241 caggagcggg tgagtcgtgt tctggaggag gaacgccttg agaaagagca gcagaaggaa
      301 gcgctgaaac agcaggaggc cgagaaaaag gtctattgcg aacggttccg gaaccggatg
      361 gagcggctgg aggcgtcatc ccaggtctac tccgagaaca aggatggcac cgtcaaatac
      421 tggaaagaca aggacgcgga tcgctacaag gccaaacagc gggagcagta tcaggcggaa
      481 tgcggcacca gctgaggctg gctggggtgc acctcccggc acacccgcgg ccagagccgg
      541 aatcacttgc ccagtgccac cttcatgaac tcgggcaccg ccagggcgcc gcggtgaatg
      601 tccacgttgt agtagcgggt ggcgaaggtt ttggcggctg attcgtcatc ccggaagcgg
      661 gtcaccgaac cctggggact ggccatggtg caggaccacc agccggtcgg gtacaccggc
      721 tgcggaaacg gcagggtatg gcactgctgg aagccggcag ttttcatgtc ctggtgaatc
      781 ttcttgatta tggtgtccgc gtgcaggagg ggtgactcgc tctgctgcac cagaatgccc
      841 tccggtttca gcgccttgcg gcaatccttg tagaaatcca ccgcgaacag tccttccgcc
      901 gggcctaccg gatcggtgct gtcgatgatg atcacatcca ggctgccggg cgcggcgttg
      961 cgaacccagg caatgccgtc atcgaacagc aattcggcgc ggggatcatg gttggcatcg
     1021 cacagttccg ggaaccattt ttctgcggcc caggtgacgc gttcgtcgat gtcgatctgg
     1081 gtgcatttct cgatgcccgg atgcttcaaa acctcgcgca gggtaccgca gtcaccgccg
     1141 ccgataatgg ctacgtgctt cggttccggg tgggtaaaca gggccgggtg tgacatcatt
     1201 tcatggtata gaaagttgtc ccgttctgtt agcatcacgc agccatccag caccatcaat
     1261 ttgccgaacg tacgggtctg gtagacctcc agatactggt agcgggtctg ctcctcgtgc
     1321 agcttttcag tgacggccag actgaaggca gtgccttcgt tgccaaacac ttcggtgaac
     1381 caattttcct gggatgactt gatcatgctg gggccccgta ctgaggtgat gtctgggtgg
     1441 atagtatggc ggttgaaagc gcgcgcaacc atacctgaag cgatggcggc ggccaagttt
     1501 tttaccgtcg ggctttaatg tggctcttga aacaggaaat acaggataaa tggcgactcc
     1561 cccgattgaa gcagcccgcg acacgtacag catcgacaac tggagcgacg gctattttga
     1621 catcaatggc gaaggccaga tcgacgtggg caccggcccg gacgtgggcc gtgtggcgtt
     1681 gtctgccgtg gtggatgccg cccgggtcgc cggcctgcgc acgccgctgc tgatccgctt
     1741 caccgacatc ctgcatgacc gtgtcaccaa gctcagtggc gcgtttgccg gtgccatgca
     1801 acagctggat tacgatgccg attacacggc ggtttacccc atcaaggtga accagcagcg
     1861 ccgggtggtg gagcagattt acggtacggc ggcgggccat ccccgctgca gcgtgggcct
     1921 ggaagcgggc agcaagccgg agctgatggc ggtgctggcg ctgagccggg caggttcggt
     1981 ggttgtctgc aacggctaca aggatcgcga gttcgtgcgt ctggccctga tcggcgagaa
     2041 gatgggccgc cgcgtctata tcgtggtgga aaagatgacc gagctggcgc tggtgctgaa
     2101 ggaagcggcc gcgctgaatg tgcagccgcg catcggcctg cgcacccgtc tcagttccat
     2161 cggcaagggc aactggcaga acaccggcgg cgagaaatcc aagttcggct tgtccgccac
//
LOCUS       DASUEG010000315         6476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_540535, whole genome shotgun sequence.
ACCESSION   DASUEG010000315 DASUEG010000000
VERSION     DASUEG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540535"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="VFX11_05875"
     CDS             complement(<1..2274)
                     /locus_tag="VFX11_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEX4938182.1"
                     /translation="MKPKVHVIAPRYPFGPEHEAQAELLAGEHRWRVDPIDISKGHLC
                     VRALNSVAEALQERTSVDAVQLRVLGKHFNLTGLEVSKYVDCESGRHKKLVLKCIDAG
                     ETSAQLWEISFALRMRPSEGVEQQYDELTLPKVPARGVYTETARQERLAFLKEQTGHD
                     LQEVSATRLDPKALVSNIEALIGSVEIPVGVAGPLHIKGEHANGLFYAPMATSEGALV
                     ASATRGATALSRAGGVTARVLGQRMLRVPVFVLEDLSMAFFFAEWVKDHQHEIAEQTR
                     RYSNYANLVELQTSVLGKAVHVHFVYETGDAAGQNMTTTCTWQACQWIIQEMKRFDGL
                     RFDNFLVEANLSNDKKVTYQSFLKGRGVRVLAECVLSHEVCTQVLKVKPRQLFDAYNH
                     FVGGSIAAGMVGLNINIANVIAALFAATGQDIACVHESAIGHLHMELTDEGHIYATLM
                     LPSLVVGSVGGGTSLPQQKECLQLMGCAGPGCAHKLAEIIASFCLALDLSTLSAIASD
                     QFARAHEKLGRNRPVNWLKLADLDADFFNRGLEEAGRRELIPVEHVQLRKIVMNGSSI
                     ITELTSHKINKLVGHFPFRLHQRDQAEPLDVMVKVKPLDDEVILMMNSMAAMCDPRLA
                     QAYNTFRNNIGFKGCHVRELVVMAQLDARFRRYTPTTYFTVHDPEREAYVIVQEYMHD
                     MELMDSADDTSGWTRAHIEAAITGIAEIHSIWYGSEDELLGQPWLGEVPTAAGMKEKS
                     RLWQMLGVHAMEEFPEWF"
     gene            complement(2824..4224)
                     /gene="asnS"
                     /locus_tag="VFX11_05880"
     CDS             complement(2824..4224)
                     /gene="asnS"
                     /locus_tag="VFX11_05880"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX4938183.1"
                     /translation="MQWPLRIADLLAGKAAVGSSVTVKGWVRTRRDSKAGFSFINIHD
                     GSAFDGIQVVAEQALPNYSDEILKLGAGCSVSITGTLVASQGKEQAVEVKAETLTVLG
                     WVDDPETYPVAKKRHTFEYLRTVAHLRPRTNTFGAMARIRNSASQAIHRYFNERGFYW
                     VNTPIITASDCEGAGELFRVSTLDLHNLPRNDKGAVDFTKDFFGAESYLTVSGQLNGE
                     AYCTAMSRVYTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLKQNADMAEEFLQYVFR
                     AVLNERQDDMAFFDQHISQGVIARLESVVNAEFVRMDYSDAITVLQKSGKKFEFPVQW
                     GLDLQSEHERYLAEEYVGRPVILMNYPQAIKAFYMRANDDGKTCAAMDVLAPGIGEII
                     GGSQREERLDVLDAKMQALGLQEGLWWYRDLRRYGTIPHAGFGLGFERLLTYITGMEN
                     IRDAIPFPRVPGSALF"
     gene            complement(4331..>6476)
                     /locus_tag="VFX11_05885"
     CDS             complement(4331..>6476)
                     /locus_tag="VFX11_05885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX4938184.1"
                     /translation="VTIEALRHLAAGRLSEWLVNVKIPASVERFGGFRVGEFDGFLRP
                     LGFEREAERDYAQLAPEVRACLDAFADGMNAAMAAYGGDYPAEFLVIGALRPWHGRDC
                     LLLARASALVIALMPLENELTFDNVRNQEGDAIARILYPDAPWENAPDVAPGGGSGIP
                     DSPFDPPNMGSNNWAVAGSRTASGRPIFCNDPHVPLIPAPTFWHHVHLECPQYRVQGG
                     MYPGFPGMGWGHNGAIAWGVTTAFRDAWDIVRIERLPDDDSRYKTPGGTGTISKHREP
                     HQSRFGKVVSLEWESCEHGVIYPDWRHHDGCDLALKVAEADGALHFTGHRALFAAQTV
                     EQTQAALAQMNRGPFDFNLVYAHKDGHIAWEQIGQLPRRNKDGLFIRNASDPDSLWQG
                     YLDFAENPKIINPESGVVATANSDTDPQQFTKIGTRVHCEPRYRQQRVTDVLLQQNAH
                     DLASMQALQRDLHCFYGLELKRPLCEALELADLPAKDRVAVSLLRDWDGQFGTDSVGA
                     CLFFFTRQTLCVNIFMNVLGGESAKRFTHGQRAIPRLDRMLLDEEDRLRQLLLLRTGE
                     SLNAWIERSFRQALRKIRKVAGPQPAQWQWGNLHRVRIGTVLADVPGLSKRWLALDAP
                     MPGEANTVSPSVAIPFGGKLRAFVGASTRFICDLAHPEEAWFSHCAGPSADPDSPYYH
                     SLPQQWLRYEYFKSALWAADAVPDVIERHVMQ"
BASE COUNT         1319 a         2052 c         1846 g         1259 t
ORIGIN      
        1 gaaccactcg ggaaactctt ccatggcgtg cacacccagc atctgccaca ggcggctctt
       61 ttccttcatg cctgcagcag tgggcacttc gcccagccac ggctggccca gcagttcgtc
      121 ctcactgccg taccagatgg agtgaatctc ggcaatgccc gtaatggcgg cctcgatatg
      181 ggcgcgggtc cagccactgg tgtcgtccgc cgaatccatc agttccatgt cgtgcatgta
      241 ttcctgcacg atcacatagg cctcgcgctc gggatcatgc acggtgaaat aggtggtggg
      301 cgtgtagcgg cggaaacgtg catccagctg cgccatcacc accagttcac gcacatgaca
      361 gcccttgaag ccgatgttgt tgcggaaggt attataggcc tgcgccagac gcggatcgca
      421 catggccgcc atgctgttca tcatcaggat gacttcatcg tccagcggtt tgaccttcac
      481 catcacatcc agcggctcgg cctggtcgcg ctggtgcaga cggaacggga aatgccccac
      541 cagcttgttg atcttgtgcg acgtcagctc ggtgatgatg ctgctgccgt tcatgacgat
      601 cttgcgcagc tgcacatgct ccaccggaat cagttcgcgg cggccggctt cttccagccc
      661 acgattgaag aaatccgcat ccaggtcggc cagtttcagc cagtttaccg gacggttgcg
      721 gccgagtttt tcgtgggcgc gggcaaactg gtcactggcg atggcgctca gtgtcgacag
      781 atccagcgcc agacagaacg atgcaatgat ttccgccagt ttgtgcgcgc accccggacc
      841 ggcacagccc atcagttgca gacattcctt ctgctgcggt aggctggtac cgccaccgac
      901 gctgccaacc accaggctcg gcaacatcag cgtggcatag atatggcctt cgtcggtgag
      961 ctccatgtgc agatggccga tggccgattc atgcacgcag gcaatgtcct ggccggtggc
     1021 ggcaaacagc gccgcgatca cgttggcgat attgatattg aggcccacca tgcccgccgc
     1081 aatactgccg ccgacaaaat ggttgtaggc gtcgaacagc tggcgcggct tcaccttcag
     1141 cacctgggtg cagacttcgt ggctgagcac acactccgcc agcacgcgca caccgcggcc
     1201 tttcaaaaag gattgatagg tgactttctt gtcgttggag aggttggctt ccaccaggaa
     1261 gttgtcgaaa cgcaaaccgt cgaaacgctt catttcctga atgatccact ggcacgcctg
     1321 ccaggtgcag gtagtcgtca tgttctggcc ggctgcatcg cccgtttcat agacgaagtg
     1381 cacgtgcacg gccttgccca ataccgacgt ctgcagttcc accagattgg cgtaattgga
     1441 atagcgtcgc gtctgctcgg cgatttcgtg ctggtgatcc ttgacccatt cggcgaagaa
     1501 gaacgccatc gacagatctt ccagcacgaa caccggcacc cgcagcatgc gctggcccag
     1561 cacccgtgcc gtcacaccgc ccgcccgcga cagcgcagtc gcaccccggg tggccgatgc
     1621 caccaacgcg ccttcggacg tggccatcgg cgcgtaaaag aggccattgg cgtgctcgcc
     1681 cttgatatgc agcggccccg ccacacccac cggaatttcc accgacccga tcagcgcctc
     1741 gatattgctg accagggctt tcgggtccag ccgggtggcc gatacttcct gcaggtcgtg
     1801 accggtctgt tctttcagga acgccaggcg ctcctgccgc gccgtttccg tataaacgcc
     1861 ccgtgccggc accttcggca gggtcagttc gtcatactgt tgctccacgc cttcactggg
     1921 gcgcatgcgc agcgcaaaac tgatttccca caactgggcc gacgtctcac cggcatcaat
     1981 gcacttcagt accagctttt tgtggcgccc gctttcgcag tcaacgtact tgctgacttc
     2041 aagacctgtc agattgaaat gcttgcccaa cacccgcaac tggacggcat ccacactggt
     2101 tcgttcctgc agcgcttccg ccacagaatt aagggcccgc acacagaggt ggcccttgga
     2161 aatatcaatg ggatcaactc gccagcgatg ttcacctgcc agcaactcag cctgcgcttc
     2221 atgctcggga ccaaagggat aacgtggtgc aatgacatgt acttttggtt tcatcacaga
     2281 ctactcgggt tgtgaaacgt gaagaaaaag gttcttcaca agggacacat ccgctgtgat
     2341 atttcgacat tcaactggcg agcagatccg cgccgcagcc gtaagttgac tcgaccaaat
     2401 gcaaaccaaa cgggaacgct gcaacgcacg gccgcagcga gctgttcaca aacaatcaat
     2461 ggtaaacaat caggaagtgc gcgagcacag tgcctggttt tctgccgcca ggctactagg
     2521 gaaattattt tagtgagacc tgattaaaaa atcgctagtc attcacaatt tgaaacgcat
     2581 ttagtgaaat ccgtttacga tgcccccggc cgtgtgcggg aaccgcaaat acagtcacga
     2641 ttgatttgaa cggacgtgct gacagaaacc gattggcgcg ctgccaaata attgaccggg
     2701 acaataaaca aaagcgccat gcaatggcaa tgacgctttt gttctggcag cctggcgtca
     2761 aaacagagaa gccgattgcc gtggtcgcca acctgaacgt ttatctattc ggggaacgcg
     2821 ccatcagaac agggcactgc ccggcacccg cggaaacgga atcgcatcgc ggatattctc
     2881 catgccggtg atgtaggtga gcaggcgctc gaagcccagg ccgaaccctg catggggtat
     2941 ggtgccgtaa cggcgcaggt cacggtacca ccagaggcct tcttgcagac ccagagcctg
     3001 catttttgcg tccagcacgt ccaggcgttc ctcgcgctgg ctgccaccga tgatctcgcc
     3061 gatccccggt gccagcacgt ccatcgcggc gcaggtcttg ccgtcgtcgt tggcacgcat
     3121 gtagaacgcc ttgatcgcct gcgggtagtt catcaggatc accggccggc ccacatattc
     3181 ctcagccaga taacgttcgt gttccgattg caggtccagc ccccactgca cgggaaattc
     3241 aaattttttg ccggattttt gcagcacggt gatcgcgtcg ctgtaatcca tgcgcacgaa
     3301 ctcggcattc accaccgact ccagccgcgc aatcacgccc tggctgatgt gctggtcgaa
     3361 gaacgccatg tcgtcctgcc gctcattgag cacggcacga aacacgtatt gcaggaattc
     3421 ctccgccatg tcggcgttct gtttcagatc ggcgaacgcc acttccggct ccaccatcca
     3481 gaattccgcc agatggcgac tggtgttgga attctcggcg cgaaacgtgg ggccaaaggt
     3541 gtaaacgcgc gacatggccg tgcaataggc ttcaccattc agctggccgg atacggtgag
     3601 gtaggattca gcgccgaaaa aatccttggt gaaatccact gcgcctttgt cattgcgtgg
     3661 caggttgtgc agatccagcg tactcacccg gaacagctcc cctgcccctt cacaatcgct
     3721 cgcggtgatg atcggcgtgt tcacccagta aaaaccgcgt tcgttgaaat agcggtgaat
     3781 agcctgggag gcagaattgc ggatgcgtgc catggcgcca aaggtgttgg tgcgcggccg
     3841 cagatgggcc accgtgcgca ggtattcgaa ggtgtggcgt tttttcgcga cgggataggt
     3901 ttccggatca tccacccagc ccagcacggt cagcgtctca gctttcactt ccaccgcctg
     3961 ctccttgccc tgggatgcca ccagcgtacc ggtaatgctg acgctgcagc cggcacccag
     4021 cttcaggatt tcgtcgctgt aattcggcag ggcttgttca gccaccacct ggatgccgtc
     4081 aaaggccgaa ccgtcgtgga tgttgatgaa ggaaaatccc gccttggaat cgcgccgggt
     4141 gcgcacccag cctttcaccg tgacgctgct gcccaccgca gccttgccag ccagaagatc
     4201 cgcgatgcga aggggccatt gcataaaggt tccttatgtt tcagaaagat agagaaatca
     4261 gaaaatcctg aaacaaacat aatgcgcaaa gcgggcaacg tccagccgga ctgactattt
     4321 caaccaggca ctattgcatc acatgccgct cgatcacatc cggcaccgca tccgcagccc
     4381 acagcgctga cttgaagtat tcataccgca accactgctg cggcagcgaa tggtagtagg
     4441 gcgaatccgg atcggccgac ggccccgcac aatgggagaa ccaggcttcc tccggatggg
     4501 ccagatcgca gatgaaacgc gtactggcgc ccacaaacgc ccgcagtttc ccgccaaagg
     4561 gaatcgccac cgacgggctc actgtgttgg cttcgccggg catcggcgcg tccagtgcca
     4621 gccagcgttt gctcaggccc ggcacatccg ccagcacggt gccgatgcgc acccggtgca
     4681 gattgcccca ctgccactgc gccggctgcg gtccggccac cttgcggatt ttccgcaatg
     4741 cctgccggaa cgaacgctcg atccaggcat tcagcgattc gcccgtgcgc agcaacagca
     4801 actggcgcag gcggtcctcc tcgtccagca gcatgcggtc cagacgcgga atggcgcgct
     4861 ggccgtgggt gaagcgtttg gcggattcac cacccagcac gttcatgaat atgttcacgc
     4921 acaatgtctg ccgggtgaaa aagaacaggc aggcgcccac ggaatcggtg ccgaactggc
     4981 cgtcccagtc ccgcagcagc gacaccgcca cccgatcctt tgccggcagg tccgccagtt
     5041 ccagcgcctc acacaaaggg cgtttcagtt ccaggccata aaaacagtgc agatcccgct
     5101 gcagcgcctg catcgacgcc agatcgtggg cgttctgctg caacagcacg tcagtcaccc
     5161 tctgctggcg atagcgcggt tcacagtgca cccgggtgcc gattttagtg aactgctgcg
     5221 gatcggtgtc gctgttcgcg gttgccacca cgccgctttc cggattgatg attttcggat
     5281 tctccgcgaa atccagataa ccctgccaga gcgaatccgg atcgctggca ttgcggatga
     5341 acagcccgtc cttgttgcga cgcggcagct ggccgatctg ctcccaggcg atgtgaccgt
     5401 ccttgtgggc atagaccaga ttgaaatcga acgggccgcg gttcatttgc gccagcgccg
     5461 cctgcgtctg ctccaccgtt tgcgcggcga acagggcgcg gtggccggta aaatgcagcg
     5521 cgccgtcggc ctccgccacc ttcagcgcca gatcgcagcc gtcatggtga cgccagtcgg
     5581 gatagataac gccgtgctcg cagctttccc attccagact cacaacctta ccgaaacggg
     5641 actggtgcgg ttctcggtgc ttgctaatcg tgccggtgcc accgggcgtt ttgtagcggg
     5701 agtcgtcgtc gggcaggcgt tcgatgcgca caatatccca ggcatcgcga aacgcggtgg
     5761 tgacacccca ggcaatggcg ccgttgtggc cccagcccat gccgggaaaa ccggggtaca
     5821 tgccgccctg cacccggtac tgcgggcact ccaggtgcac gtgatgccag aaagtgggcg
     5881 caggaatcag cggcacgtgg ggatcattgc agaagatcgg ccggccgctg gcggtgcggc
     5941 tgccggccac cgcccagttg ttgctgccca tgttgggcgg atcgaacgga ctgtcgggaa
     6001 tgccggagcc gccgccgggg gccacgtccg gtgcattttc ccagggcgca tccggataca
     6061 ggatgcgggc gatggcatcg ccttcctggt tgcgcacgtt gtcgaaggtc agctcatttt
     6121 ccagcggcat cagcgcgatc accagggcgc tggcgcgggc cagcagcagg caatcgcgcc
     6181 cgtgccaggg acgcagtgcg ccaatcacca ggaattccgc cggatagtct ccgccatagg
     6241 ccgccatggc cgcattcatg ccgtcggcga acgcatccag acaggcgcgc acctcgggtg
     6301 ccagctgcgc gtaatcccgc tccgcctcgc gctcaaaacc cagcggacgc aggaagccgt
     6361 cgaactcacc cacccggaaa ccaccgaagc gctccacgct ggccggtatc ttcacgttca
     6421 ccagccactc cgacaaccga ccggccgcga gatgccgcag ggcctcgatc gtcacc
//
LOCUS       DASUEG010000316         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_426935, whole genome shotgun sequence.
ACCESSION   DASUEG010000316 DASUEG010000000
VERSION     DASUEG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426935"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFX11_05890"
     CDS             complement(<1..272)
                     /locus_tag="VFX11_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX4938185.1"
                     /translation="MQIGIPAEIVAGETRVAATPETVKKYVQLGYSVVVQTGAGVASS
                     CTDSAYQEAGATIAPTAADTYKDADLVLKVRAPQAEEIALIKSGAAV"
     gene            complement(531..800)
                     /locus_tag="VFX11_05895"
     CDS             complement(531..800)
                     /locus_tag="VFX11_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4938186.1"
                     /translation="MSNKRRHQRTNLRCKFKIWNDSIGEVLVTTRDISDGGVFLILDS
                     TINLPIGTQARGQVQGMMADAPIVAMEVVRMEPTGVGLKFIPDPQ"
     gene            997..1959
                     /locus_tag="VFX11_05900"
     CDS             997..1959
                     /locus_tag="VFX11_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX4938187.1"
                     /translation="MASSRHWTEHNIPDLSGQRVLVTGANSGIGFETARVLAQRNAHV
                     ILACRTEAKAVEAMARIRALYPRAQLQFLPLDLASQTSVREMVAMFREQYDTLDLLIN
                     NAGVMWLPQSKTVDGFESQLGTNHLGHFALTGLLLPVLMNTPGARIVTVSSIAHRGGQ
                     IHFDDLWLDRGYGRQKAYSQSKLANLVFALELQRRLHAAGAPIISVAAHPGVANTNLA
                     IPGFEQGGSPLLARVVSLLTPVVAQNPVNGALPTLYAATSHAVEGGDYIGPNGLYEMY
                     GYPRKAYATRRSRNPEIGRRLWDLSEQLTGVRFDFTPAIGVRRG"
     gene            complement(1981..2379)
                     /locus_tag="VFX11_05905"
     CDS             complement(1981..2379)
                     /locus_tag="VFX11_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX4938188.1"
                     /translation="MNSNDTHSKLIGYILWIFGFTGSHRFYYGKPITGTIWFFTLGLL
                     GIGWIIDLFLIPGMDRAADRKYRTGSTDYTVAWILLTFLGYFGIHRFYMGKIITGLIW
                     LLTAGVFGLGWLYDYWTLNGQIDELNRAEG"
     gene            complement(2404..2886)
                     /locus_tag="VFX11_05910"
     CDS             complement(2404..2886)
                     /locus_tag="VFX11_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012143011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4938189.1"
                     /translation="MNISKDKVVQFHYTLKNDKGEEIESTIGKDPMAYLHGHGNIIPG
                     LESALEGKAAGDRFTITIEPKDGYGERQDVEWQRIPLKHLQGARKWKPGMMAFVETEQ
                     GYRQVTVMKVGKFNADVDVNHPLAGKTLTFDIEVLDLRDATDDEKSHGHAHGVGGHHH
                     "
     gene            complement(2934..>3476)
                     /gene="rlmJ"
                     /locus_tag="VFX11_05915"
     CDS             complement(2934..>3476)
                     /gene="rlmJ"
                     /locus_tag="VFX11_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX4938190.1"
                     /translation="SPAIAAQLLREDDRLRLVEMHSSDVNHLEQNLGEDPRVKVISGD
                     GFEQIRALLPPSARRGLVLIDPPYELKEDYQYVLQGVQEGLKRFATGTFLIWYPLLST
                     TASQKFSGQLQKLHANWLNVQLKVSAKPYGHGMYGSGMFVINPPWVLRDQLLEGLPYL
                     VKLLAQDDAAEFTLEASPAT"
BASE COUNT          687 a         1038 c         1021 g          730 t
ORIGIN      
        1 acggcggcgc cgctcttgat cagggcgatt tcctcggcct ggggcgcacg gaccttcaat
       61 accaggtctg catctttata ggtgtctgcg gcggtaggcg cgatggtggc tcctgcttcc
      121 tgataggcgc tgtcggtaca gctggaagcg acccccgctc cggtttgcac gaccacggaa
      181 tatccgagct ggacgtactt tttcaccgtt tcaggagtgg cggccacacg cgtttcgcca
      241 gcgacgattt ccgctggaat cccaatctgc atggtttacc ctcactaatg ttgacgtttg
      301 ttaagcctgc accccagcga aagctgtccc cggtgcaatt cggacacgca aacacaccgg
      361 gaccccggcc tgttgatcag gccagggcac tggcgataat gggctgaatc tgtttccggc
      421 gcgaagggta accacttata agatgaaagg ctgtggcgtt ccttcgctgg atcggccgag
      481 gtcggtaggg tatatgcctt ttggggtcct tgcaaccctt cggaacgggc ctattgaggg
      541 tcgggaatga acttcaatcc cacgccggtg ggctccatgc gaaccacttc catggccacg
      601 atcggcgcgt ccgccatcat cccctgcacc tgccccctgg cctgggtacc gatcggcaga
      661 ttgatggtgg aatcgagaat caggaatacc ccgccatcgg aaatatcgcg ggtggtcacc
      721 agaacctcac caatggaatc gttccagatc ttgaatttgc agcgcaggtt cgtgcgctga
      781 tgacggcgct tgttggacat ggatgtactg cttcctgaag ggattgcttt cagtctagca
      841 gccagtggca aaccttctac aggcggcgat atgccaatgg cgacgggcta aaggccccgc
      901 cggccgaaat cacacaaacc tgtcgccgcc caccattgcc gctgccgaaa tgccgctgct
      961 agcatgcggg ttcgactgag cggagagaaa cgcaacatgg caagcagcag gcactggacc
     1021 gaacacaata ttcccgacct gagcggacag cgggtgctgg tcaccggcgc caacagcggc
     1081 attggttttg aaacggcgcg ggtactggcc cagcgcaatg cccacgtgat cctggcctgc
     1141 cgcaccgaag ccaaggcggt ggaagcaatg gccaggattc gcgcccttta tccccgggcg
     1201 cagctgcagt ttctgccgct ggacctggcc agccagacat cggtacgcga aatggtggcg
     1261 atgtttcgcg agcagtacga cacactggac ctgctgatca acaacgccgg cgtgatgtgg
     1321 ctgccgcaat cgaaaaccgt cgatggtttt gaaagccagc tgggcaccaa tcatctgggc
     1381 catttcgcac tgaccggatt gctgttgccc gttctgatga atacgcccgg agcgcgcatc
     1441 gttaccgtga gcagcattgc ccatcgcggc ggccagattc attttgatga cctctggctc
     1501 gaccgcggtt acggccgcca gaaggcctat tcccaaagca agctggcgaa tctggtgttc
     1561 gcactggaat tgcagcgccg cctgcatgcc gccggtgcgc ccatcatttc ggtggcagcg
     1621 catcccggcg tggccaatac caatctggcc ataccgggct ttgaacaggg cggctcgccg
     1681 ctgctggccc gggtcgttag cctgctgaca cccgtagtcg cacaaaatcc ggtgaacggc
     1741 gccctgccta cgctgtacgc ggcaacaagt catgcggtag aaggcggcga ctatatcggg
     1801 ccaaacggcc tgtacgagat gtatggatat ccgcgcaagg cgtacgcaac gcggcgctcg
     1861 cgcaatcccg agatcgggcg tcgtctgtgg gacctttcgg aacagttgac gggtgtgcgg
     1921 tttgatttca cgcccgctat cggtgtccgg cgcggataac gccggacacg acgggaacgg
     1981 tcaaccctcg gcccggttca gctcatcgat ctggccgttc agggtccagt agtcgtacaa
     2041 ccagcccagc ccgaacacac cggccgtcag cagccagatc agtccagtga tgatcttgcc
     2101 catgtaaaag cggtgaatac cgaagtaacc caggaaggtc agcagaatcc aggccacggt
     2161 gtagtcggtg ctaccggtcc tgtatttgcg gtctgccgcc cgatccatgc cagggatcag
     2221 aaacaggtcg atgatccagc cgattccaag caggcccagg gtgaaaaacc agatggtgcc
     2281 ggtgatgggc ttgccgtaat aaaagcggtg cgatccggtg aaaccaaaaa tccacagaat
     2341 gtagccaatg agtttggaat gggtgtcgtt gctgttcatg ggattccttc tccgttgcgc
     2401 tgatcagtga tgatgtccac ctacaccatg ggcatgaccg tgggattttt cgtcatcggt
     2461 ggcatcgcgc agatccagca cttcgatatc aaatgtcagc gtcttgcctg ccagcggatg
     2521 gttcacgtca acgtcggcgt tgaacttgcc tactttcatc acggtgacct gacgataacc
     2581 ctgctcggtt tcaacgaatg ccatcatgcc cggcttccac ttgcgcgcgc cctgcaggtg
     2641 cttgagcgga atacgctgcc actccacatc ctggcgctcg ccgtaaccat ccttgggttc
     2701 gatggtgatg gtgaacctgt cgcccgcggc cttgccttcc agcgcacttt ccaggccggg
     2761 aatgatgttg ccgtgaccgt gcaggtaggc catcggatcc ttgccgatgg tggattcgat
     2821 ttcctcaccc ttatcgttct tcagggtgta atggaattgc acgaccttgt ctttgctgat
     2881 gttcatggga atgtcctgcc tgcttgatat tgaatgcgca ttaagtgggt aaatcaggtg
     2941 gccgggctgg cctccagcgt gaattcggcc gcgtcgtcct gcgccagcag cttcaccaga
     3001 tagggcagac cttccagtaa ctggtcccgc aagacccagg gcggattgat gacgaacatg
     3061 ccactgccgt acatgccatg gccgtagggc ttggcgctga ctttcagctg cacgttcagc
     3121 cagttggcat gcagcttctg caactgcccg gagaatttct gcgacgccgt cgtgctgagc
     3181 agtggatacc agatcagaaa ggttccggtg gcaaagcgct tcagcccttc ctgcacgccc
     3241 tgcagcacgt actggtagtc ctccttcagt tcgtagggcg gatcgatcag caccagaccc
     3301 cgtcgggcgc tgggcggcag cagggcccgg atctgctcga aaccgtcacc actgatcact
     3361 ttcacccgcg ggtcttcgcc caggttctgc tccaggtgat tgacgtcgga actgtgcatc
     3421 tccaccagcc gcaggcggtc gtcctcccgc agcaattgcg cagcgatggc cgggga
//
LOCUS       DASUEG010000317         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1947259, whole genome shotgun sequence.
ACCESSION   DASUEG010000317 DASUEG010000000
VERSION     DASUEG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947259"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFX11_05920"
     CDS             complement(<1..1303)
                     /locus_tag="VFX11_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4938191.1"
                     /translation="MTTLWPRSLLKRILLAGSLLLATPLCAATEAAPSVSVSFYKKLG
                     EAHDWLQKKHQENARAILQKLTEQANNSAYENGLVWKMLGYLHYQNGDYKQSAQAFEK
                     ALQFDIPTVLAQDVRKLLGQVYLADGQYEKAAQHLNRWLTQATAEQQASAWDVRLALA
                     QCYYQLKQYQKVVTHLSAVIRQYETEGRQPKEEWLSLLQASLAQTDAVQERIETIKRL
                     LTWYPKAEYWMALAGAYGQMDKMQDYLATLSVARRKDLLRSESQYVSLASVFYSHDVP
                     MSAAAVMEEGLRRNIISANAKNLRFLAACYTQAQEFEKALVPLQQAAGKSEGGEIDAM
                     LGNAYFQLARWPDAANALEAAIQKGELKQLATVWLLLGQAYLNQQQYERAVYAFRQAS
                     QDESRARQAKQWMQYAEYEKDRHAQLGLIKSVPGTAQEPNHE"
     gene            complement(1300..1980)
                     /locus_tag="VFX11_05925"
     CDS             complement(1300..1980)
                     /locus_tag="VFX11_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4938192.1"
                     /translation="MKPLPRYLIACAAGGIVTFVLFALMIGLIKISEQPVASGDFEMV
                     DFIRVEHDRSLNLEQMKPLPPPQPQAPPERVSMQIGGVDVTAQRVAPGGTGPITEGLA
                     LSGGIAAGSGGFGSGEGSGSGFGTGFGDGAVGGGDYLPVVQVPPQYPPAALKQGIEGW
                     VLVEFTIGTEGQVKDARVVKSEPPGIFDRPALDAVQRFRFRPRTMGPLAVEVTGVQNR
                     IRFRLEKP"
     gene            complement(1985..2392)
                     /locus_tag="VFX11_05930"
     CDS             complement(1985..2392)
                     /locus_tag="VFX11_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4938193.1"
                     /translation="MRRPFYRATEDEDLGIDLTPMLDIVFIMLIFFVVTASSGRDTGI
                     DIQRPKARTAVTKDATHLRVMISEYDEIWVDKRRVDARALKPQLERMHFDSPQSALII
                     YADKKSTNNRLVQVMDAAREVGINNISIAASEQ"
     gene            complement(2392..2934)
                     /locus_tag="VFX11_05935"
     CDS             complement(2392..2934)
                     /locus_tag="VFX11_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4938194.1"
                     /translation="MWALIEALETIRDFHFAGGAVLWTIGFVTVLMWVFIIERSLYFR
                     LTLPREMEAIVAQWYARSERHSWHAHQIRRMLIAQVTASVDFSLPVLRMLVALCPLFG
                     LLGTVTGMVEVFDVMNAFGTANPRSMASGISRATLPTMAGMGAAIYGLFASRLLCRHA
                     ANQVELLADRLTLSSPQRST"
     gene            complement(2936..>4287)
                     /locus_tag="VFX11_05940"
     CDS             complement(2936..>4287)
                     /locus_tag="VFX11_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4938195.1"
                     /translation="LFAGSAPVFAAGNGSKPEIQSLDELVELVRQGRIEQSKLNQQRE
                     QLFLRNQNQQKQLLDQAIAQRKQLEARSAQLEEAHNIQKQELEELSRRLETHMGSLKE
                     LFAHLQSASGETAAQMGLSLTSVDSPDRQKSLTQLADRLGPNRSLPRIEELEQLWYQQ
                     QREMVESGKVKHLSLPVINLEGERIQADVVRIGNFNLVSGNHYLQFIPETGKVVELPK
                     QPPRYMLGNVEDFTQSDSSAGLLPIAVDPTRGTLLNMLLEMPDMRERIDQGGIIGYFT
                     IVIGALGILLAFERIFVLVIVTRKVADQKRHEKPSNDNPLGRILLTYETNRHLEPESL
                     ELKLGEAILRERAPLEKYLTLIKIVSVVAPLLGLLGTVTGMINTFQVITLFGTSDPKL
                     LAGGISEALVTTVIGLVVAIPTVFFHNLASTRSKAVLMVLEEESTGLIAQRAEQRAA"
BASE COUNT          756 a         1303 c         1316 g          912 t
ORIGIN      
        1 tttcatgatt cggttcctgg gcagtgcctg ggacggactt tatcagaccc agttgcgcgt
       61 ggcggtcttt ttcatattcc gcgtactgca tccactgttt ggcctgcctg gcgcgggatt
      121 catcctggct ggcctggcga aaggcataca cggcccgctc gtactgttgc tggttgaggt
      181 aggcctgccc cagcagcagc cacacggtgg caagctgctt gagttcgccc ttctggatcg
      241 ccgcttccag cgcgttggcc gcgtccggcc agcgtgccag ctgaaaatag gcgttgccca
      301 gcatggcatc gatttcacct ccctcggatt tgccggcggc ctgttgcagc ggtaccagcg
      361 ccttttcaaa ttcctgcgcc tgggtgtagc aggcggcgag aaaacgcagg ttcttggcgt
      421 tggcactgat gatgttgcgg cgcaggcctt cttccatcac cgcggcggca ctcatgggca
      481 cgtcgtggga ataaaaaacg ctggccagcg atacatactg cgattcgctt cgcagcagat
      541 ccttgcgtcg cgccaccgac agcgtggcca ggtaatcctg catcttgtcc atctgaccgt
      601 aggcgccggc gagggccatc cagtattcgg ccttgggata ccaggtcaac aggcgcttga
      661 tggtttcgat gcgttcctgc accgcatctg tctgcgccag gctggcctgc agcagcgaca
      721 gccactcctc cttcggctgg cgtccttcgg tttcatattg gcggattacg gcggagaggt
      781 gggtcaccac tttctggtat tgcttcagct ggtaataaca ttgggccagt gcgagccgca
      841 cgtcccaggc gctggcctgc tgctcggctg tggcctgggt cagccagcgg ttcaggtgct
      901 gcgccgcctt ttcatactgg ccatcggcca ggtacacctg ccccagcagt ttgcgcacgt
      961 cctgcgccag cacggtaggg atatcgaact gcaatgcttt ttcgaacgcc tgcgcgctct
     1021 gcttgtaatc gccgttctgg taatgcaggt aaccgagcat tttccagacc agaccatttt
     1081 cgtaggcgct gttattggcc tgctcggtga gcttctgcaa aatggcgcgc gcattttcct
     1141 ggtgcttttt ctgcagccag tcgtgggcct cgccgagttt tttatagaag gataccgaaa
     1201 cgctgggcgc ggcttccgtg gcagcgcaca gtggtgttgc cagcagcagg ctgccggcca
     1261 acagaatgcg tttcagcagc gaacggggcc acagcgtcgt catggttttt ccagccggaa
     1321 acgaatgcgg ttctgcacgc cggtgacttc caccgccagc gggcccatcg tgcgtggccg
     1381 gaagcggaag cgttgcacgg cgtcgagggc gggccggtcg aaaatgccgg gcggttcgga
     1441 tttcaccacc cgcgcatctt tcacctgccc ttcagtgcca atggtgaatt ccaccagcac
     1501 ccagccttca ataccctgct tgagtgccgc tggcggatac tgcggtggta cctgcacgac
     1561 gggcaggtaa tcgccgccgc ccactgcacc gtcgccaaag cccgtgccga atccgctgcc
     1621 actgccttcc ccgctgccga agccgccact gccggcagcg atgccgccgg aaagcgccag
     1681 gccctcggtg atgggaccgg ttccgcccgg tgcgacgcgc tgggccgtca catccacacc
     1741 accgatctgc atgctcaccc gttcgggcgg tgcctggggc tggggtggcg gcagcggttt
     1801 catctgctcc agattcagtg agcggtcatg ctcgacgcgg atgaagtcca ccatttcgaa
     1861 atctccgctc gccaccggct gctcactgat tttgatcagg ccgatcatca gcgcgaacag
     1921 cacgaaggtg acaattcctc cggcggcaca ggcgatgaga tagcgtggca gcggtttcac
     1981 ggcatcattg ctcgctggct gcaatggaaa tgttgttgat gccgacttcc ctggccgcat
     2041 ccatgacctg caccagccgg ttattggtgg acttcttgtc ggcataaatg atcagtgcgc
     2101 tttgggggct gtcgaagtgc atccgctcta gttgcggctt cagtgcccga gcgtccaccc
     2161 ggcgcttgtc tacccagatc tcgtcatatt cgctgatcat cacccgcaga tgggttgcat
     2221 ccttggtgac ggcagtgcgc gccttcggac gctggatgtc gatgccggta tccctgcctg
     2281 atgatgcagt gacgacgaaa aagatcagca tgatgaagac gatgtcgagc atcggagtaa
     2341 gatcgatgcc gagatcctcg tcttcggtgg cgcgataaaa cgggcgacgc atcaggtgga
     2401 cctctgcggt gaggaaagag taagccggtc ggcgagcagt tccacctggt tggcagcgtg
     2461 ccggcacagc agccggctgg cgaacagacc gtaaatggca gcgcccatgc cggccatggt
     2521 gggcagcgtc gcgcgcgaga tgccggaggc catggagcgt ggattggccg ttccgaatgc
     2581 gttcatcacg tcaaacactt ccaccatgcc ggtgacggtg ccgagcaatc cgaacagcgg
     2641 gcagagcgcc accagcatgc gcaacaccgg cagcgaaaaa tccacgctgg cggtgacctg
     2701 ggcgatcagc atgcgacgga tctggtgggc atgccaggaa tgccgttcac tgcgcgcgta
     2761 ccattgcgcc acgatggctt ccatttcgcg cggcagcgtc aggcggaagt acaggctgcg
     2821 ctcgatgatg aacacccaca tcagcacggt gacgaaaccg atggtccaga gcactgcgcc
     2881 acccgcaaaa tgaaaatcgc ggatggtttc cagagcttcg atcagcgccc acatgtcagg
     2941 cggccctctg ctcggcgcgc tgcgctatca ggccggtgct ctcctcttcc agcaccatga
     3001 gtactgcttt gctgcgcgtg ctggccaggt tgtggaagaa caccgtggga atcgcgacca
     3061 ccagaccgat cacggtggtg accagcgctt cggaaatacc gccggccagc agtttgggat
     3121 cgctggtgcc gaacagcgtg atgacctgga aggtgttgat cattcccgtc accgtaccca
     3181 gcaatcccag cagcggtgcc accacggaca cgatcttgat cagggtgaga tatttttcca
     3241 gcggcgcccg ttcacgcagg atcgcttcgc ccagtttcag ttccagcgat tccggctcga
     3301 gatgacgatt ggtttcatag gtcagcagaa tccgtcccag cggattgtcg ttgcttggct
     3361 tttcatgccg cttctggtct gccaccttgc gggtgacgat aaccagtacg aagatgcgtt
     3421 cgaacgccag cagaatgccc agcgcaccga tgacgatggt gaagtagccg atgatgccgc
     3481 cctggtcgat gcgttcgcgc atgtccggca tttccagcag catgttcagc aacgtgccac
     3541 gggtgggatc gaccgcaatg ggcagcaggc cggcgctgct gtcgctttgg gtgaaatcct
     3601 cgacattgcc cagcatgtag cggggcggct gtttgggcag ctccaccacc ttgccggttt
     3661 cgggaatgaa ctgcaggtag tggttgcccg acaccaggtt gaaattgccg atgcgcacca
     3721 cgtcagcctg gatgcgttcg ccttccagat tgatcaccgg cagcgacagg tgcttgacct
     3781 tgcccgattc caccatctcg cgctgctgct ggtaccagag ctgttccagt tcctcgatgc
     3841 gcggcaggga gcggtttggg cccaggcggt ccgccagttg cgtcagtgac ttctggcgat
     3901 cgggtgaatc caccgaagtg agcgaaagtc ccatttgcgc ggcggtttca ccggaggcgg
     3961 actgcagatg cgcaaacagt tccttcagcg agcccatgtg cgtttccagg cgacgcgaca
     4021 attcctccag ctcctgcttc tggatgttgt gggcttcctc cagctgggcg ctgcgggctt
     4081 ccagttgctt gcgctgtgcg atggcctggt ccagcagctg cttttgctgg ttctggttgc
     4141 gcaggaacag ttgctcccgc tgctgattca gcttcgactg ctcgatgcgg ccctggcgca
     4201 ccagttccac cagttcgtcg agggattgga tttccggctt gctgccgttg ccggcggcga
     4261 ataccggcgc gctgccggca aacagga
//
LOCUS       DASUEG010000318         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_180878, whole genome shotgun sequence.
ACCESSION   DASUEG010000318 DASUEG010000000
VERSION     DASUEG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180878"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFX11_05945"
     CDS             <1..382
                     /locus_tag="VFX11_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4938196.1"
                     /translation="NAGANLTTPVFTGGKADAAEASATAARAMALASYQQSILTALRE
                     VNDALTRQHHARTLLEGRQLQQQAMDKTLRLAQLRYKNGYSGYLEVLTVQGQLYQMQM
                     AVLESQRARLSAAVSLYKALGGGW"
     gene            505..1868
                     /locus_tag="VFX11_05950"
     CDS             join(505..982,982..1868)
                     /locus_tag="VFX11_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612104.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX4938197.1"
                     /translation="MAKYKESLKRQLVKKYLSGSCGLRGLERQHGVSRRTLATWVARF
                     RIHGEAGLSKKHEYYRADFKELVVHHMRREALSFNDAAVLYNIRGGGGVVATWVRLYD
                     EGGIEALQSKPRGRRTTMSESKPIPAAAPAAAEARTVEELLKENEYLRAEVAYLKKLG
                     CLGSGQTASRAEKAQAVNELRQTHSLPVLLEVGKLSRSTFYYQLKALAAEDKHAALKD
                     KIRQIYTHHQGRYGYRRITDALRMAGDLVNHKTVQQLMQGLGLKSLVRPRKYRSYRGE
                     AGALAPDLLKREFHARAPNQKWVTDVTEFNVKGEKLYLSPVMDLFNGEIVAYQIEKRP
                     VLGLVTRMLGQALSRLTPTQKPLLHSDQGWHYQHASYRKMLSDRQLVQSMSRKGNCLD
                     NAAMESFFATLKAEFFYLNTFNSTEQLRDGIHRYIEYYNHQRIKTKLKGLSPVQYRTQ
                     ALAA"
     gene            complement(1933..>2129)
                     /locus_tag="VFX11_05955"
     CDS             complement(1933..>2129)
                     /locus_tag="VFX11_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938198.1"
                     /translation="ADLPYALPAGEWSLLLRSTDDTPLSRPMKIKDKQILVPATSILL
                     LSAPPMKDNPLLAKEVQQHG"
BASE COUNT          487 a          609 c          638 g          395 t
ORIGIN      
        1 gaatgccggc gccaacctga ccacgccggt atttaccggc ggcaaggcgg atgctgccga
       61 agccagcgcc accgccgcgc gcgccatggc cctggccagc taccagcagt ccattctcac
      121 cgcgctgcgc gaagtcaatg acgccctgac gcgtcagcac cacgcgcgta ccctgctgga
      181 aggacgtcag ttgcaacagc aggcgatgga caaaaccctg cgtctggcgc agttgcgcta
      241 caaaaacggc tactccggct atctggaagt gctgacggtg cagggtcagc tgtaccagat
      301 gcagatggcg gtgctggaaa gccagcgcgc ccgcctgtcg gccgcggtga gcctttacaa
      361 ggcgctgggc gggggctggt aatccggcga agcgttgcct gacgcgcgaa ccgcggcctg
      421 gacctgccag aaaaaagccc cgccggaagc aattgaactg caccccaaaa gttggacatc
      481 gatccaacct ttggggtgtt tttcatggcg aagtacaaag agagtctgaa gcgacaactc
      541 gtgaagaagt acctgtccgg cagttgcggg ctaaggggtc ttgaacggca gcatggagta
      601 agccgaagga cgctagccac atgggtggcc cgttttcgga tccacggcga ggcaggtctg
      661 agcaagaagc atgaatacta tcgtgctgac ttcaaagaat tggtagtgca ccatatgcgt
      721 cgtgaggcgc tgtcatttaa cgatgccgcg gtgctgtaca acatccgggg cggtggcggg
      781 gtcgttgcga cttgggttcg gctgtatgat gaaggcggta tagaagccct tcagtccaag
      841 ccacgaggcc gccgcacgac catgtccgaa tcgaagccca ttcctgccgc ggcacccgca
      901 gccgcagaag cccgtaccgt ggaagaactc ctcaaggaga acgaatacct gcgcgcggag
      961 gtggcgtacc taaaaaaatt ggatgccttg gttcgggcca gaccgccagc cgcgccgaaa
     1021 aagcgcaagc ggtgaacgaa ctgaggcaaa cgcatagcct gccagtattg ctggaggtcg
     1081 gtaagttatc gcgcagcacg ttctactatc agttgaaggc actggcagcc gaggacaaac
     1141 acgcagcgct caaggacaaa attcgtcaga tttacaccca tcaccaagga cgttatggtt
     1201 atcgacgcat cacggacgcg ctgcgcatgg cgggtgatct tgttaaccac aagaccgtgc
     1261 agcaattgat gcagggtctg ggattgaagt ccctggttcg tccccggaag tatcgcagct
     1321 atcggggtga agcaggcgcc ttggcccccg acctgctcaa gcgcgagttc cacgcaaggg
     1381 cccccaacca gaagtgggtg acggatgtga cggagttcaa cgtgaagggc gagaagcttt
     1441 acctgtcgcc agtgatggat ctgttcaacg gtgaaatcgt cgcctatcag atcgagaagc
     1501 gccccgtact cggtttggtg acgcggatgc tggggcaagc actgtctcgg cttacgccca
     1561 cgcaaaaacc gttgctgcat tccgatcagg gctggcacta ccagcacgcc agctaccgaa
     1621 agatgctatc cgaccgtcag cttgtgcaga gcatgtcgcg aaaagggaat tgcctggaca
     1681 acgcggccat ggagagcttc ttcgctacgc taaaggccga gttcttttac ctgaacacct
     1741 tcaacagcac cgagcaattg cgggacggca tccatcgcta catcgagtat tacaaccacc
     1801 aacgcatcaa gaccaaacta aaaggcctga gtccggtaca gtaccgaact caggccttgg
     1861 ccgcctagct tttaaactgt ccaacttctt ggggtcagtt caccgaatgg ggttttttaa
     1921 tcgacgacgg gcttaaccgt gttgctggac ttccttcgcc agcagcgggt tgtccttcat
     1981 cggaggcgcc gacagcagca gaatcgaggt ggccggcacc aggatttgct tgtccttgat
     2041 cttcatcggc cggctcagcg gtgtgtcgtc agtggagcgc agcagcagac tccactctcc
     2101 agccggcagt gcatagggca gatcggcca
//
LOCUS       DASUEG010000319         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1105942, whole genome shotgun sequence.
ACCESSION   DASUEG010000319 DASUEG010000000
VERSION     DASUEG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105942"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFX11_05960"
     CDS             complement(<1..606)
                     /locus_tag="VFX11_05960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4938199.1"
                     /translation="MESKPILERLHPALIPSLAVLPEHLPVVLLTRHSIREEPANRFA
                     TYEVPLTPEGVALAHALGERLPRPVSRLYSSPVGRCVATAQAIAEGAGVQLPVETRST
                     LVEPGSYVQDIAQVGGLFFKLGPLGFANKHLRNEVRGVLSPQQGALQMLQHLQQSLGE
                     PGSLSVHVTHDTILASFIYFLRGQRELDQSPWPWMMEGAFLW"
     gene            748..1458
                     /locus_tag="VFX11_05965"
     CDS             748..1458
                     /locus_tag="VFX11_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938200.1"
                     /translation="MNTLTRRQREFLQREQLFLDAARQILRSDGVAAFTIDRIASNTE
                     YAKGTVYKHFTCKEDILCALCHESLVHLGEAFSKALTFPGNSRERMMALGTAYLQFTS
                     RFPEEFDILIAVRTNNVRQKASEKRVEAMIAADQGAHDVMRSVMGAAIAAGDLTLPPA
                     TSLDEACFGLWAMAFGLLALAPASDMIDALQLPSVPAIMVNQLNLLLDGYGWRPLSTE
                     FDYRQTFTRLLNHMETTK"
     gene            1469..>2940
                     /locus_tag="VFX11_05970"
     CDS             1469..>2940
                     /locus_tag="VFX11_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4938201.1"
                     /translation="MYSPIAVWLVQKRLPLFIASLLALAVSLYGYSLLSYSSDYRVFF
                     EKDNPQLVANETIQATFSRSDNILLVLEPKNRDSFTPDNLAAVEWLTEQAWQLPHSQR
                     VDSLTNFQHTYVDGDDLYVEDMFSNAHDMDPAEIARRKEQALKEPLLVHRLVSTSGHV
                     SAVNIELGLPDEQTVATARVMEESRALRDEFLAKYPQFNAYITGVTPLNHAFDEVARS
                     DGETLMPIMFLLILVMVGLMLRSVASTLITLSVIITGVAITIGLTGLVGIQLNNINSV
                     APIIIMTLAVADCVHLLSHYLTARRKGMDKVTAMTDALDVNFMPVFLTSFTTMIGFLS
                     MNSSDSPPFQTFGTISAIGVFFTWLFSLTVLPQLAIWFSRATPSIDTERTALFHRLGG
                     FTIRHPRKLFFGAIGLGLFTFIFIGDNDLNDDNFGYFSKNIEVRQGSDFAEANLNGLN
                     LIEYALDSKEENGISDVAFLKQVDAFAQWYRQQPEVTHVFT"
BASE COUNT          617 a          943 c          820 g          560 t
ORIGIN      
        1 ccagaggaag gcgccttcca tcatccaggg ccaggggctc tggtccagct cccgctggcc
       61 gcgcagaaaa tagatgaagg acgccagaat ggtgtcatgg gtgacatgca ccgacagact
      121 gcccggttca cccaggctct gctgcaggtg ctgaagcatc tgcagcgcac cctgctgtgg
      181 cgacagtacg ccgcgcacct cgttgcgcaa gtgcttgttg gcaaaaccca gcggccccag
      241 cttgaagaac aggccaccca cctgcgcgat gtcctgcacg tagctgccgg gttccaccag
      301 cgtggagcgg gtttccaccg gcaactgcac gccggcgccc tcggcaatgg cctgggccgt
      361 ggcaacacag cgtcccaccg ggctggaata cagtcgcgac accggtcgcg gcaagcgctc
      421 acccagcgcg tgcgccaggg ccacgccttc cggggtcagc gggacttcat aggtggcaaa
      481 gcggttggca ggctcctccc ggatggaatg ccgcgtcagc agcactaccg gcaggtgttc
      541 cggcaacacc gccagggacg gaatcagggc cggatgaaga cgctccagaa ttggtttgct
      601 ttccatgtgg ttagccagtg gatgcaggtg aatcgggtca accatcacag catgcctgat
      661 tgacaacggc accgccagat gcaaaatgtg acgcatcgtc acgttttgac ggacaatcac
      721 acgcatgcct actcacaaga gcacttcatg aataccctga cccgacgcca gcgggaattc
      781 ctgcagcgcg aacagctgtt cctcgacgcc gcccgccaga tcctgcgcag cgatggcgtg
      841 gcggcgttca ccatcgaccg catcgccagc aataccgaat atgccaaggg caccgtctac
      901 aagcatttca cctgcaagga agacatcctc tgcgccctct gccacgaaag cctggtccac
      961 ctgggggaag ccttcagcaa ggcactgacc ttccccggca actcacggga gcgaatgatg
     1021 gcgctcggca ccgcttacct gcagttcacc agccgctttc ccgaggagtt cgacattctt
     1081 atcgccgtgc gtaccaacaa tgtccgccag aaagcgtcgg aaaagcgggt ggaagccatg
     1141 atcgccgccg accagggcgc gcatgacgtg atgcgatccg tgatgggcgc cgccatcgcc
     1201 gccggcgatc tgacactgcc cccggcgacc agcctggacg aagcctgttt cgggctctgg
     1261 gccatggcct tcggcctgct ggcactggca ccggccagcg acatgatcga tgcgctgcag
     1321 ctgccttctg tcccggctat catggtcaat cagctgaacc tgttgctgga cggttatggc
     1381 tggcgccccc tcagcaccga attcgattac cggcaaacgt ttacccgcct gctcaatcac
     1441 atggaaacaa cgaagtaagg acgcccttat gtattcaccc attgctgtct ggctggtaca
     1501 aaaacgcctg cccctgttca tcgccagcct gctggccctg gccgtcagcc tgtatggcta
     1561 cagtctgctg agctattcct ccgactaccg cgtctttttc gaaaaggaca acccgcaact
     1621 ggtggccaat gaaaccatcc aggccacctt ctcgcggtct gacaacatcc tgctggtgct
     1681 ggaacccaaa aatcgcgaca gcttcacgcc tgacaacctc gcagcggtgg aatggctgac
     1741 agagcaggcc tggcaattgc cccattccca gcgtgtggat tccctcacca atttccagca
     1801 tacctatgtc gacggtgacg acctgtatgt tgaagacatg ttcagcaatg cgcacgacat
     1861 ggacccggcg gaaattgcac gccgcaagga acaggcgctg aaggaacccc tgctggtgca
     1921 ccggctggtg tccaccagcg ggcacgtgtc ggccgtgaac atcgaactgg gcctgccgga
     1981 cgaacagacg gttgcaaccg cacgggtaat ggaagaaagc cgggccctgc gggatgaatt
     2041 cctggcgaaa tatccgcagt tcaatgccta catcacgggc gtcacaccgc tgaatcatgc
     2101 attcgacgaa gtggcccgca gcgacggcga aaccctgatg cccatcatgt tcctgctgat
     2161 cctggtgatg gtgggcctga tgctgcgctc cgtggccagc acgttgatta ccctgagcgt
     2221 catcatcacc ggcgtggcca tcaccatcgg gctgaccgga ctggtgggca tccagctcaa
     2281 caatatcaac agcgtggcac cgatcatcat catgacgctg gcggtggcgg actgcgtgca
     2341 cctgctgtcg cattacctga cagcacggcg caagggcatg gacaaggtca ccgccatgac
     2401 cgacgcgctc gatgtgaatt tcatgccggt gttcctcacc agtttcacca ccatgatcgg
     2461 attcctcagc atgaacagca gcgattcgcc gccgttccag accttcggca ccatttccgc
     2521 gatcggtgtg ttcttcacct ggctgttttc actcacggtg ctgccgcaac tggcgatctg
     2581 gttcagccgc gccacaccgt ccatcgacac cgaacgcacg gcactgtttc atcgtcttgg
     2641 tggcttcacc atccggcatc cgcgcaagct gttttttggt gccatcggcc tggggctctt
     2701 cacttttatt ttcatcggcg acaacgatct caacgacgac aacttcggct atttcagcaa
     2761 aaacattgaa gtgcgccagg gctcggattt tgccgaagcc aacctcaatg gcctcaacct
     2821 gatcgagtac gcgctggaca gcaaggaaga aaacggcatc agcgatgtgg ccttcctgaa
     2881 gcaggtggat gcgttcgcgc aatggtatcg gcagcaaccg gaagtcaccc acgtttttac
//
LOCUS       DASUEG010000320         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1393359, whole genome shotgun sequence.
ACCESSION   DASUEG010000320 DASUEG010000000
VERSION     DASUEG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393359"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFX11_05975"
     CDS             <1..710
                     /locus_tag="VFX11_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938202.1"
                     /translation="CLGANSFLRLEALLRWHHPARGLISPAVFVPVAEESDLINQIGN
                     WVLEEACRQARQFMDRSIQVVMSVNVSARQLRAADFVQRVQQVLADTGLDPSQLELEV
                     TETTLLDNMETRAESLQQLQDLGVAIALDDFGSGFSSLNYLKNLPVDRLKIDQVFIQE
                     IPHDEESKTITASVVKLAHELHLTVVAEGVETWDQLRFLQSVGCDYVQGFLLFRPLPP
                     EEIGAVFASLASLRFDA"
     gene            complement(790..1374)
                     /locus_tag="VFX11_05980"
     CDS             complement(790..1374)
                     /locus_tag="VFX11_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008077955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX4938203.1"
                     /translation="MAFTLPELPYAKDALAPHISAETLEFHHGKHHNAYVTNLNNLIP
                     GTEFEGKSLEDIIKTSSGGVFNNAAQIWNHTFYWHSLSPKGGGAPKGAVADAITKTWG
                     SFDKFKEEFNKMAVGNFGSSWTWLVKKADGSLAIVNTSNAGTPITEKGVTPLITVDLW
                     EHAYYIDFRNLRPKYLEAFWALVNWDFANANLAK"
     gene            complement(1516..2871)
                     /gene="mgtE"
                     /locus_tag="VFX11_05985"
     CDS             complement(1516..2871)
                     /gene="mgtE"
                     /locus_tag="VFX11_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX4938204.1"
                     /translation="MAKSANLETQDQLQTFLEALDSGTFADVRAMLHGMSAAEIAHLI
                     ESSPPRERNLLWQLLDKDDEGEVLQYLNEEIAAQFLQHRNAEEIISAVEGFDADDFAD
                     LLQELPHKVMKRVLESMDEQNRHRIEAILSFPEDTAGGLMNTDIITIRPDISLDVVLR
                     YLRRHESLPEATDTLLVVNRSGKFVGTLPLTTLLVKDPVMLVREAMITDATAIRADQS
                     AKDVASIFEREDLVSAPVIDREGVLLGRITVDDVVDVILEGADHSLMSMAGLDEYEDT
                     FAPVLRSTRRRAVWLGINLATAFLASAVVGLFEHTLEKVVALAVLMPVVASMGGIAGT
                     QTLTLVIRGMALGHVGSANARWLLTREMAVGFLNGLMWAVVVGAVASAWFNDYTLGGV
                     LAVAMIINLVMAAAAGAALPMILQRMGIDPALSGGVILTTFTDCVGFFAFLGLATVMF
                     A"
     gene            complement(2985..3257)
                     /locus_tag="VFX11_05990"
     CDS             complement(2985..3257)
                     /locus_tag="VFX11_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX4938205.1"
                     /translation="MELKKQLIIINKLGLHARAASKLVATASKFDCSVQINRNGQIAD
                     AKSIMSVLMLAASKGTVLDFTVNGHDAEEALLQIETLINNRFDEGE"
     gene            complement(3299..>4054)
                     /gene="rapZ"
                     /locus_tag="VFX11_05995"
     CDS             complement(3299..>4054)
                     /gene="rapZ"
                     /locus_tag="VFX11_05995"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX4938206.1"
                     /translation="LPVTLLQHLMKELTHSNPPVTMAAIGIDARNTSRQLQKLPNVLR
                     DLIGDGVDSQVIYLDADDETLIKRFSETRRKHPISSDTKTLREAIERERDMLNPVASS
                     ASLIIDTSTLTLHQLRDLIKVRVGEAADTMAVLVESFGFKNGIPKDADLVFDVRCLPN
                     PHWITHLRAKTGLEQDVQDFLAGHETVGEMIADILAYLDKWLPRFAANNRSYMTVAIG
                     CTGGQHRSVYITEILLQHLRQRFSNVQARHREL"
BASE COUNT          729 a         1162 c         1279 g          884 t
ORIGIN      
        1 tctgcctggg tgccaattca ttcctgcggc tggaagcgtt gttgcgctgg catcatccgg
       61 cgcggggttt gatcagtccc gccgtgttcg tgccggtggc ggaggaatcc gatctgatca
      121 accagattgg caactgggtg ctcgaggagg cctgtcgcca ggcacgccag ttcatggatc
      181 gcagtattca ggtggtgatg tcggtgaatg tgtcagcgcg gcagctgcgt gcggctgact
      241 tcgtgcagcg ggtgcagcag gtattggccg acactggcct ggacccctcg caactggagc
      301 tggaggtgac cgaaaccacg ttgctggaca acatggaaac ccgcgccgaa tcgctgcagc
      361 aattgcagga tcttggtgtg gccatcgcgc tggacgattt cggttccggc ttctcttcgc
      421 tgaactacct gaaaaacctg ccggtggacc ggctcaagat cgatcaggtt ttcattcagg
      481 aaattcccca cgacgaagaa agtaaaacca tcaccgcatc ggtggtgaag ctggcccacg
      541 aactgcattt gaccgtggtg gcggagggcg tggaaacctg ggatcagctg cgtttcctgc
      601 aaagtgttgg ttgtgactat gtgcaggggt ttctgctgtt ccggccgctg ccaccggagg
      661 aaatcggcgc ggtgttcgcc agtctggcca gcctgcgctt cgatgcctga ctgcgccaca
      721 aacagaaacg ccggccttgc ggggccggcg atctgtcagc tgaggaggct gtctgcgtaa
      781 cgggcgtgct tacttggcca gattcgcgtt ggcgaaatcc cagttcacca gggcccagaa
      841 ggcttccagg tatttcgggc gcaggttgcg gaaatcgatg tagtaggcgt gttcccacag
      901 gtcgacggtg atcagcgggg tgacgccttt ctcggtgatc ggcgtgccgg cgttgctggt
      961 gttgacgatc gccaggctgc cgtcagcctt cttcaccagc caggtccagc tggaaccgaa
     1021 gttgcccacc gccatcttgt tgaactcttc cttgaacttg tcgaacgagc cccaggtttt
     1081 ggtgatggcg tctgccaccg cacctttggg agcgccgccg cctttggggc tcaagctgtg
     1141 ccagtagaac gtgtggttcc agatctgcgc cgcgttgttg aacacgccgc cgctggacgt
     1201 cttgatgatg tcttccaggc tcttgccttc gaactcggtg ccggggatca ggttgttcag
     1261 gttggtcacg taggcgttgt ggtgcttgcc gtggtggaat tccagggttt ctgcagaaat
     1321 atggggcgcc agtgcgtctt tcgcataggg cagttcggga agtgtgaatg ccatgagtat
     1381 gtccctctcg tttttgggtc tttaacaggt ttaagaacgc cagactttag cagattaggg
     1441 gggcgatgtc agatggtggc aggcatccgg gcggttttga ttttccaatc gaaaccatag
     1501 tttttttcga tgaattcagg cgaacatgac cgtggccaga cccaggaatg caaagaatcc
     1561 gacacagtcg gtaaacgttg tcaggatgac gccaccggac agggccgggt cgatccccat
     1621 ccgttgcagg atcatcggca gcgcggcacc ggccgccgcc gccatgacca gattgatgat
     1681 catggcgaca gccagtacgc cgcccagggt gtaatcgttg aaccaggccg aggccacggc
     1741 gcctaccacc acggcccaca tcaggccatt cagaaatccc accgccattt cccgggtcag
     1801 cagccagcgc gcgttggccg atcccacatg gcccagcgcc atgccgcgga tcaccagggt
     1861 cagggtctgg gtgccggcga tgccgcccat gctggccact accggcatca gcaccgccag
     1921 tgccaccact ttctccaggg tatgttcgaa cagccccacc acggctgacg ccaggaatgc
     1981 agtggcgaga ttgatgccca gccagactgc gcggcgccgg gtggagcgca gcacgggggc
     2041 aaaggtgtcc tcgtattcgt ccaggcccgc catgctcatc agcgagtggt ccgcgccttc
     2101 cagaatgacg tcgaccacgt cgtccaccgt gatccggccc agcagtacac cttccctgtc
     2161 gatgaccggc gcgctcacca ggtcctcgcg ctcgaagatg gaagccacat ccttggcaga
     2221 ctggtcggcg cggatggcgg tggcatcggt aatcatggcc tcgcgcacca gcatgacggg
     2281 gtctttcacc agcagcgtgg tcagcggcag ggtgccgaca aacttgccgg aacggttcac
     2341 caccagcagg gtgtcggtgg cttccggcag ggattcatgc cggcgcagat agcgcagtac
     2401 cacgtcgagc gagatatccg gcctgatggt gatgatgtcg gtattcatca ggccgccggc
     2461 ggtgtcttcc gggaacgaca ggatcgcctc gatgcgatgg cggttctgct cgtccatgga
     2521 ttccagcacc cgcttcatca ccttgtgcgg cagttcctgc agcaggtcgg caaagtcgtc
     2581 ggcgtcgaaa ccttccaccg cgctgatgat ctcttccgca ttgcgatgtt gcaggaactg
     2641 cgcggcgatt tcctcgttca ggtactgcag cacctcgcct tcgtcgtcct tgtccagcag
     2701 ctgccacagc agattgcgct cacggggcgg cgacgactcg atgaggtggg cgatttcggc
     2761 ggccgacatg ccatgcagca tggcgcgcac gtcggcaaac gtaccgctgt ccagcgcttc
     2821 gaggaacgtc tggagctgat cctgggtttc aagattggca ctcttcgcca tgcgggaact
     2881 cacgggtgcg ggctggtgtt ggcggctgtt ctttcagtgt agccagtctg gcagtttatc
     2941 cagtcaggca ccttggccgg tttcgatctg ctgggtgggg cggatcattc gccttcgtcg
     3001 aagcggttgt tgatgagggt ttcgatctgc agcagcgcct cctctgcatc atgaccgttc
     3061 acggtgaaat ccagcacggt ccccttgctg gccgccagca tcagcactga catgatgctt
     3121 ttggcatcgg caatctggcc attgcggttg atctgcacgc tgcaatcgaa tttcgacgcc
     3181 gtcgccacca gctttgacgc tgcgcgcgca tgcagaccca gcttgttgat gatgatgagc
     3241 tgttttttca gttccatggg gagcaaggct tcagaaaatc tgaaaggagt atcgcaagtt
     3301 acagctcgcg atgtctagcc tgaacgttgc tgaaacgctg acgcagatgc tgcagcagga
     3361 tttcggtgat gtagacggag cggtgctggc cgccagtgca gccgatggcc accgtcatgt
     3421 agctgcggtt gttggcggcg aaacgcggca gccatttgtc caggtacgcc agaatgtcgg
     3481 caatcatttc gcccacggtt tcgtggccgg ccagaaaatc ctgcacgtcc tgctccagcc
     3541 cggtcttcgc gcgcagatgg gtgatccagt ggggattggg caggcagcgc acgtcgaata
     3601 ccagatcggc atccttggga atgccattct tgaagccaaa ggattccacc agcacggcca
     3661 tggtgtccgc agcctccccc acgcgtacct tgatcaggtc gcgcagctga tgcagtgtca
     3721 gcgtgctggt atcgatgatc aggctggcgc tggaggccac tggattcagc atgtcccgtt
     3781 cccgctcgat ggcttcgcgc agggttttgg tgtcgctgct gatggggtgt ttgcggcggg
     3841 tttcgctgaa gcgcttgatc aacgtctcgt cgtcggcgtc caggtagatc acctggctgt
     3901 cgacaccgtc gccgatcagg tcacgtaata cattgggcag cttttgcagt tgccggctgg
     3961 tgttgcgcgc gtcgatgccg atggcagcca tggtgacggg cggattggaa tgtgtcagct
     4021 ccttcatcag gtgctgcaac agggtgaccg gaag
//
LOCUS       DASUEG010000321         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1218488, whole genome shotgun sequence.
ACCESSION   DASUEG010000321 DASUEG010000000
VERSION     DASUEG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218488"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1018
                     /gene="imuA"
                     /locus_tag="VFX11_06000"
     CDS             185..1018
                     /gene="imuA"
                     /locus_tag="VFX11_06000"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEX4938207.1"
                     /translation="MTTHGSPLNLLRHPAVWRAREAPLPAQRAGQGVATGFAPLDRAL
                     PDSGWPVSGLAELLCPHWGCGEAELLLPLLRQLSAQQRWLVWINPPWLPFAPALARQG
                     VALEHTLLLRCERDKDLLWAMEQCLASGSCSLVQGWPARPQPQQIRRLQLAAQKGHSL
                     GLLLRPSACVKQPSPAPLRLELARHAASVQVRVVKCRGNWGSDWLALQAPVAVGREPV
                     ADAVVSGQAAFPASMDLGTGSAASVLQVLPRLKMDAARTPAIATASGWNAGLTPPVSG
                     P"
     gene            1021..2514
                     /locus_tag="VFX11_06005"
     CDS             1021..2514
                     /locus_tag="VFX11_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX4938208.1"
                     /translation="MLWACLHFPALALNRVEQTLPAPRALVIEITRRQRRLVWQANAP
                     ARAAGISPGMTIPSAQGLCRDLLCVPRNETAEQDALRELGNWAYQFTPHIQLPGSQTQ
                     KGQLQASNSLLLEVGHSLRLFHSKENLAQQLLEKMPAGFQPCHLAFCDTAFAALLLAQ
                     AQDGATVDSAVPKNPALPFFSLQDLPAQSVRCLHLADDKIALLESMGIVTLEHLFALP
                     HDALAKRFGPDFMLYLRRLTGAVPDILPSWQLPDQFRATLEFLHELDTTDQLLFPLRN
                     LLTRLQHYLQARQLATTELHFILTLRNRTLQHWPLGLAAPHYRSEHMLPLLQLKLAQL
                     QLQAPVLALQLAVNRFVELPRGQQDLLTPWQSDQVSRQQLVDRLKARLGDANVCGLGM
                     VADHRPEKSWAACAPGTGKKQEPLEEQRPFWLLPTPQKLRDKKGLPLHEGELQLLKGP
                     ERIDTGWWDDQPVNRDYYVARQPGGRLLWIYRDRVDQHWYLHGIFGE"
     gene            2592..>3129
                     /locus_tag="VFX11_06010"
     CDS             2592..>3129
                     /locus_tag="VFX11_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX4938209.1"
                     /translation="MTAHYAELHCFSNFSFLHGASRPEELVQQAVALNYSALAITDEC
                     SVAGVVRAHVEAKKLNLKLIIGSDFTSEEGLHFIVLAPDRRAYGQLTTLISRARRRSE
                     KGEYCVRVADLQPVTDACLFIWLPAVTQCVQPNAHCANFMNSTRSRLWIGLHNNLGAA
                     DENTCLARMQFAQRLGLPL"
BASE COUNT          545 a          949 c         1023 g          612 t
ORIGIN      
        1 gattgcgttg ccatcggtct ttccgatcca ccatttcatg ctggcagcga cgatggagat
       61 gggccagttt ccatggaccg gaagtcgtga tgcgtggcga ggggatcggg tcctaagcgg
      121 ctttcttggg tgatgctgct atactgtata taattacagt attttccctt tgagaaacct
      181 ggccatgacg acccacggat ctccactcaa cctgttgcgc catcctgcgg tctggcgcgc
      241 gcgggaggct cccctgcctg cccagcgcgc tggacagggt gttgccacgg gttttgcccc
      301 gctggatcgc gccctgcccg acagcggttg gccggtgagc gggctggcgg aactgttgtg
      361 cccgcactgg ggctgcggtg aggcggagct gttgctgccg ctgctgcgcc agttgagtgc
      421 ccagcagcgc tggctggtgt ggatcaatcc gccctggctg ccgttcgcgc cggcgctggc
      481 ccggcagggg gtggcgctgg agcacacgct gctgctgcgc tgcgaacggg acaaggatct
      541 gttgtgggcg atggagcagt gcctggcgtc gggcagttgc agcctggtgc agggctggcc
      601 ggcgcggccc cagccgcaac aaatccgccg tctgcaactg gcggcacaga agggccacag
      661 cctgggcctg ctgttgcggc catcggcttg cgtgaagcaa ccgtcgcctg cacccctgcg
      721 actggaactg gcgcggcatg ccgcaagcgt gcaggtgcgg gtggtgaaat gccggggcaa
      781 ctggggctcg gactggctgg cattgcaggc gccagtggct gtgggacggg agccagtggc
      841 cgatgccgtg gtgtccggtc aggcggcttt tcctgcgtcc atggacctgg gcacgggttc
      901 ggctgcaagc gtgctgcagg ttctgccccg gctgaaaatg gatgccgcca gaacgccggc
      961 cattgccact gccagtggct ggaacgccgg cctcacgcca cccgtctctg ggccctgacc
     1021 atgctctggg cctgtctgca ttttcccgca ctggcgctga accgggtgga gcaaaccctc
     1081 cccgccccgc gtgcgctggt gatcgaaatc acccggcgtc agcgtcggct ggtgtggcag
     1141 gccaatgcgc cggcccgcgc cgccggcatt tcaccgggca tgacgattcc ctccgcgcag
     1201 ggtttgtgcc gcgatctgtt gtgtgtgccg cgcaacgaaa ccgccgagca ggacgcgctg
     1261 cgggaactgg gcaactgggc ctaccagttc acgccgcata ttcaattgcc aggtagccag
     1321 acgcaaaaag gccagttgca ggctagcaac agcctgctgc tggaagtggg acacagcctg
     1381 cgcctgtttc acagcaagga aaatctggcg cagcaactgc tggaaaaaat gcccgccggt
     1441 ttccagccct gtcatctcgc gttttgcgac acggcttttg ccgcgctgct gctggcgcag
     1501 gcgcaggatg gtgcgacagt cgattccgcc gtgccaaaaa accctgcact gccgtttttt
     1561 tcgctgcagg atctgcctgc gcaaagcgtg cgctgtctgc atctggcgga cgacaagatt
     1621 gcgctgctgg aaagcatggg catcgttacg ctggaacact tgttcgcact gccccatgac
     1681 gccctggcga aaagattcgg cccggatttc atgctgtatc tgcgccggct gaccggtgcg
     1741 gtgccggaca ttctgccgag ctggcagctg ccggaccagt tccgcgccac gctggaattc
     1801 ctgcacgaac tggacaccac cgaccagctg ctgtttcccc tgcgcaatct gctgacgcgg
     1861 ttgcagcatt atctgcaggc acggcagctg gccaccaccg aactgcattt cattctcacc
     1921 ctgcgcaatc gcacattaca gcactggcct ttggggctgg ccgcgcccca ctatcgcagc
     1981 gaacacatgc tgccgctgct gcaactgaag ctggcgcagc tgcaattgca ggcgccggtg
     2041 ctggcgctgc aactggcggt gaaccggttt gtggaactgc cacggggcca gcaggatctg
     2101 ctgacgccct ggcaaagtga ccaggtcagc cgccagcagc tggtggacag gctgaaagcg
     2161 cgtctgggcg acgccaatgt gtgcgggctg ggcatggtgg cggatcaccg gccggaaaaa
     2221 agctgggccg cctgtgcgcc gggcacgggg aaaaagcaag agccattgga ggaacaacgt
     2281 ccgttctggc tgctgcccac gccgcaaaaa ctgcgcgata aaaaaggcct gccactgcac
     2341 gagggcgaac tgcaactgct gaaaggcccg gagcgcatcg acaccggctg gtgggacgat
     2401 cagccggtga accgcgatta ttatgtggcg cggcaaccgg gcggccgcct gttgtggatt
     2461 taccgcgacc gcgttgacca gcactggtat ctgcacggaa tattcgggga gtaaggcctg
     2521 atgaacccgg ccattggccc tgctgattgc cacgcttttt ccgcattctt cggatgttcg
     2581 gtgagatcgc catgactgcg cattacgccg aactgcactg cttttccaat ttcagcttcc
     2641 tgcacggcgc ctcgcggccg gaggaactgg tgcagcaggc ggtggcactg aactattccg
     2701 cgctggccat caccgatgaa tgctctgttg ccggtgtggt gcgggcccat gtggaagcga
     2761 aaaagctgaa tctgaagctg attatcggca gcgatttcac ttcagaagaa ggcctgcatt
     2821 ttatcgtgct ggcaccggac cgtcgtgcct atggccagct caccaccctg atcagccggg
     2881 cgcggcgacg cagcgagaaa ggcgaatact gcgtgcgggt ggcggatctg caaccggtta
     2941 cggatgcctg cctgtttatt tggttgccgg ctgtgacgca gtgcgtgcag ccaaatgcgc
     3001 actgcgccaa ttttatgaac agcacgcgtt cacggttgtg gattggcctg cacaacaatc
     3061 ttggtgcggc cgatgaaaat acgtgtctgg cgcgcatgca gtttgcgcaa cgcttgggtc
     3121 tgccactgg
//
LOCUS       DASUEG010000322         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2036879, whole genome shotgun sequence.
ACCESSION   DASUEG010000322 DASUEG010000000
VERSION     DASUEG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036879"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFX11_06015"
     CDS             <1..350
                     /locus_tag="VFX11_06015"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4938210.1"
                     /translation="FSIGLGIVVDDTVHILSRHIRNRRAGMSPLASLEHVLEETGSAL
                     VLTTVVLTCGMGILTASIFGPNQTMAILMASNILIALLYDLLMMPHMLVLLDRWIFPK
                     LVEAETAALPVAS"
     gene            505..1854
                     /locus_tag="VFX11_06020"
     CDS             505..1854
                     /locus_tag="VFX11_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX4938211.1"
                     /translation="MKWLWVIAAVAAAALCYFALPVAPPVRAGLTITVLIGVLWLTEA
                     IDITFSALLIPVLAIAFQVLPAKQAFADFANPVIFLFMGGFALAAALARHGIDRAIGQ
                     GVLKIAGTHPVRAAYLLFAATAFISMWISNTATAAMMLPVALGIASRFHDPDRKTAMF
                     LLLGTAYAASLGGMATLVGSPPNAIAASHQSITFAEWMTFGMPTALLLMPLMVGLLQL
                     LLRPSFELLPADTLMPAVTSSQPAQPARQRQFTLLIFAGVVCGWIFSSPLSALTGVSK
                     EFDAWVAVIAILALGLSGTLKWEDIEKKTGWGILLLFGGGMTLSTVLEQTGTSTYIAG
                     QLKDLVHGAPTMVALLVMITFIVFLSELVSNTACAALLIPLLVPVSAHFGVEPKVLAV
                     LVAFAASAAFMLPVATPPNAIVFGTGLLPQRVMMRCGFFLNLICISVLGTLAWLFWI"
     gene            complement(1929..>3080)
                     /locus_tag="VFX11_06025"
     CDS             complement(1929..>3080)
                     /locus_tag="VFX11_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938212.1"
                     /translation="PTLTVYGLEIHGQTKKNGSSVYTNDVAVRVSAQVGLVLGLGADN
                     KLAVSFRQAPSVKVEGVRVGEGAWSGPVINAAANLLVSTAIGVVMDELARPIENIKLP
                     TFACMAFESVNISAVGGEGGHLNLAGTLTQVSDECQNEVTPPPAVAYGRGVGVPMSCG
                     SNQEYDAGLCYQQCAEGYNGVGPVCWKEEASYGRGVGSIPNLCAAGEEMDAGLCYPVC
                     SPGYDGVGPVCWSTRPLSYGRGVGTIPTNIWTGQCPTGKENQAGLCYWYCDSGYTGVG
                     PVCYLNNASYGRGVGTIPKTCPAGQENDGGLCYPVCQQGYHGVGPVCWTNSALSYGRG
                     VGTVPNTCHDGWERDGLLCYQQCAEGYNGIGPVCWPVETQQATLSPIPQ"
BASE COUNT          551 a          997 c          901 g          631 t
ORIGIN      
        1 ccttcagcat cggcctgggc atcgtggtag acgacaccgt gcacattctc agccgccata
       61 tccgcaaccg tcgcgccggc atgtcgccac tggccagtct tgagcatgtg ctggaggaaa
      121 ccggctcggc gctggtgctg accaccgtgg tgctgacctg cggcatgggc attctgacgg
      181 cgtcgatatt cgggcccaac cagaccatgg cgatcctgat ggcatccaac atcctgatcg
      241 cactgctcta cgacctgctg atgatgccgc acatgctggt gctgctggat cgctggattt
      301 tccccaagct ggtggaagcg gaaacagcgg ctctgcctgt tgcttcctga caattgcgac
      361 accactgccc tgctgcgggt ttgacgcgcc gcatttgcag ggcgagccag acttgctaca
      421 gtccagccat tcctggctga ctggcgcatc cccatgcaaa ccgctacatc tgcacctgct
      481 gttcctcccg ccgcttcagg ctccgtgaaa tggctctggg tcatcgccgc cgtggcggct
      541 gccgcgctgt gttattttgc gttgcccgtt gcgccgcccg tccgcgccgg cctgaccatc
      601 acggtgctga tcggcgtgct gtggctgacg gaagccatcg acatcacctt cagcgcactg
      661 ctgatccccg ttctcgccat cgcattccag gtgctgccgg caaaacaggc ctttgcagat
      721 ttcgccaatc cggtgatttt tctgttcatg ggcgggttcg cgctggcggc ggcgctggcc
      781 cgtcatggca tcgaccgcgc catcggccag ggtgtgctga aaatcgcggg cacccatccg
      841 gtgcgggcag cctatctgct gtttgccgcc accgcgttca tttccatgtg gatcagcaat
      901 acggcaacgg ccgcgatgat gttgccggtg gcgctgggca tcgcgtcgcg ttttcatgat
      961 ccagaccgca aaaccgcgat gtttctgctg ctgggcactg cctatgccgc cagtctgggg
     1021 ggaatggcaa cccttgtggg cagcccgccc aacgcgattg ctgccagcca ccagtccatt
     1081 acgtttgcag aatggatgac gttcggaatg ccaacggccc ttttgctgat gccgctgatg
     1141 gtgggcctgc tgcaactgct actacgacct tcatttgaac tgttgcccgc cgacaccctc
     1201 atgcctgccg tgacctcaag ccagccggca caacccgcgc ggcagcgcca gttcacattg
     1261 ctcatctttg ccggcgtagt gtgcggctgg attttcagca gcccactgtc ggcactcacc
     1321 ggcgtgagca aggaattcga cgcctgggtg gcggtaatcg ccatcctggc actggggctg
     1381 agcggtaccc tgaaatggga ggacatcgaa aagaaaaccg gctggggcat cctgctgctg
     1441 ttcggtggcg gcatgacatt gagcaccgtg ctggaacaaa ccggcaccag cacttacatc
     1501 gccggacaat tgaaggatct ggtgcacggg gcaccgacca tggtggcact gctggtgatg
     1561 atcaccttca tcgtgtttct gtcagaactg gtgagcaaca ccgcgtgcgc cgcactgctg
     1621 attccgctgc tggtgccggt gtcagcgcac ttcggcgtgg aaccgaaagt gctggcggtg
     1681 ctcgttgcgt tcgccgcgtc tgccgccttc atgttgcccg tggcgacacc gcccaacgcc
     1741 atcgtgtttg gcacggggct gttaccgcag cgggtgatga tgcgttgcgg tttcttcctc
     1801 aatctgattt gtatcagcgt gctgggcacg ctggcctggc tgttctggat ctgaacgatt
     1861 tcatcagcaa gcccaaaaaa aacgcctccc gaaggaggcg ttcattttca tggtgacaac
     1921 agcagctgtt actggggaat cggcgacagc gtagcctgct gtgtctccac cggccagcat
     1981 accgggccga tgccgttata gccttcggcg cactgctggt agcagagcag gccatcacgt
     2041 tcccagccat cgtggcaggt gttcggaaca gtgcctacac cacggccgta cgacaacgcc
     2101 gagttggtcc agcagaccgg gccaacacca tggtaaccct gctggcatac cggatagcac
     2161 aggccgccat cgttttcctg gcctgcgggg caggttttcg gaatggtgcc gacgccacgg
     2221 ccataggaag cattgttcag atagcacacc ggtccgacgc cggtatagcc gctgtcacaa
     2281 taccagtagc agaggcctgc ctggttttcc ttgccggtgg gacactggcc ggtccagatg
     2341 ttggtgggaa tggtacccac gccacgacca taggacagcg gacgggtcga ccagcacacc
     2401 gggcccacac cgtcataacc cggtgagcag actggatagc acaggccggc atccatttcc
     2461 tcgccagcgg cacacagatt gggaatcgag cccacaccac gtccgtagga ggcttcttcc
     2521 ttccagcaca ccgggccaac gccgttatag ccttcagcgc actgctgata gcacagaccg
     2581 gcatcgtatt cctggttgga accacaggac atcggcacgc ccacgccacg gccataggcc
     2641 acagccggcg gcggtgtcac ttcgttctgg cattcatcgc tcacctgggt cagcgtgccc
     2701 gccaaattca ggtggccgcc ctcaccgccc acggcggaga tgttcacgct ctcaaatgcc
     2761 atgcacgcga acgtcggcag cttgatgttc tcgatcggcc gcgccagctc gtccatcacc
     2821 acgccgatgg ccgttgagac cagcaggttg gcggcggcat tgatcaccgg gccggaccac
     2881 gcaccctcac ccacgcgcac gccttccact ttcacgctcg gtgcctgacg gaatgacacc
     2941 gccagcttgt tgtcggctcc caggcccagc accaggccta cctgcgcgct cactcgcacg
     3001 gcgacatcat tggtgtaaac ggacgaaccg ttcttcttgg tctggccatg aatttccagg
     3061 ccgtacacgg tcagggtcgg
//
LOCUS       DASUEG010000323         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_819525, whole genome shotgun sequence.
ACCESSION   DASUEG010000323 DASUEG010000000
VERSION     DASUEG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819525"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..266
                     /locus_tag="VFX11_06030"
     CDS             84..266
                     /locus_tag="VFX11_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938213.1"
                     /translation="MQLIVVLGVLVAINFGIVYVGSEAGKDEEPAQQGVVEVEPVADC
                     APDTDCSKPEEAPAKQ"
     gene            403..585
                     /locus_tag="VFX11_06035"
     CDS             403..585
                     /locus_tag="VFX11_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938214.1"
                     /translation="MGHVIVIFGTLIALNMGFGYWLSEQNAENQPQQGVVEVEDVQAD
                     ASAPAAAPVEETKPAE"
     gene            complement(777..1409)
                     /gene="crp"
                     /locus_tag="VFX11_06040"
     CDS             complement(777..1409)
                     /gene="crp"
                     /locus_tag="VFX11_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEX4938215.1"
                     /translation="MTIKPNSLIPDPESFLAHCHRRKYPSKSTIIYAGDDSDALYYII
                     KGTVMVIIEDDEGREMIMAYLNEGDFFGEMGLFDKTANRSAWIRAKTECEVAEISYNK
                     FKQLAKEDADILFAVSAQIAGRLRATTRKVGDLAFLDVTGRVARTLIDLCKQPEAMTH
                     PDGMQIKITRQEIGRIVGCSREMVGRVLKNLEEQGLIQVKGKTMVVYGTR"
     gene            1835..2257
                     /locus_tag="VFX11_06045"
     CDS             1835..2257
                     /locus_tag="VFX11_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4938216.1"
                     /translation="MKGTVRWAGDVKFVAESGSGHSIVIDGPPDYGGKNQGVRPMELM
                     LLGVAGCSSFDVIHILKKARQDVTDCVTAVEAERVDAVPAVFKKIHMHFVVTGRNLKD
                     SQVKRAVELSAEKYCSASIMLSKGGVEITHSYEVKETE"
     gene            2257..2676
                     /locus_tag="VFX11_06050"
     CDS             2257..2676
                     /locus_tag="VFX11_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4938217.1"
                     /translation="MGRPAAFGGLRHVALFVEKFAECEHFYVGLLGMQVEWRPDPDNL
                     YLTSGCDNLALHRAPAAIDRGSRQKLDHIGFIIDMEAAVDDWYTYLKSNGVVMRTAPR
                     SHRDGARSFYCEDPDGTMVQMIWHPPIAGLNMSRAQA"
     gene            2723..>3345
                     /gene="speD"
                     /locus_tag="VFX11_06055"
     CDS             2723..>3345
                     /gene="speD"
                     /locus_tag="VFX11_06055"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX4938218.1"
                     /translation="MKNPETPEKLKLHGFNNLTKSLSFNIYDICYAKTPEHRQEYRQY
                     VDELYNAERLTQILTTVTEIVGANILNIARQDYDPEGASVTMLIAEHPIAPTEDPSRE
                     EPGPLKESVVAHLDKSHVTVHTYPETHPDNENGISTFRVDIDVSTCGVISPLKALNYL
                     IHQFDSDIVTVDYRVRGFTRDVNGRKLYIDHEINSIQNFFLDETLDL"
BASE COUNT          714 a          896 c          985 g          750 t
ORIGIN      
        1 ctcaagggtg agcgggacac cgctccgtat accaatccaa tcacttgaat aatgaaaaat
       61 ggactttcta caggggagac agcatgcaat taatcgtggt cctgggcgta ttggtcgcca
      121 tcaatttcgg tatcgtgtac gtgggctctg aggccggcaa agatgaggag ccagcccagc
      181 aaggcgtcgt cgaagtagaa ccggtggcgg attgtgctcc tgatactgat tgctccaagc
      241 cggaagaagc acccgcgaaa caataactta cattaccctg ccagcatgcc gggccgatcg
      301 ggccgctgtt ggcagggcaa agtgactcat gaagtgactg cgggtcggtt agtatatata
      361 atgtcgacgc agtcctcttg actgcacttg aaggagattc ttatgggaca cgttatcgtt
      421 atcttcggta ccttgattgc cttgaacatg gggttcggct actggctgtc ggaacagaac
      481 gcagaaaacc agccccagca gggtgttgtg gaagtggaag acgtgcaggc ggacgcatcg
      541 gcacctgccg ccgcaccggt agaagaaacc aagccagcag aataagcctt cgggcgagcc
      601 gtggtgcggc gctggccgcg tccggaaaag atgaagagcc tcgcgtgatg cgaggttttt
      661 tttcgcccca gctttccctg ggaaatcccg ctggccacgg attgcgaacc cgttggcggc
      721 agaaatgaaa aaggcccagc gagggccttt tccgggcggc tgattcaaat gcccagtcag
      781 cgggtgccgt acaccaccat ggtcttgccc ttcacctgga tcagtccctg ttcttccagg
      841 tttttcagta cccgccccac catctcgcgg gagcagccaa cgatgcggcc gatttcctgc
      901 cgggtgatct tgatctgcat gccatccggg tgagtcatgg cttcaggctg cttgcagagg
      961 tcgatcaggg tgcgggctac gcggcccgtt acgtcaagga aagccagatc tcccaccttg
     1021 cgggtggtgg cacgcagccg cccggcgatc tgggcgctaa cggcgaacag gatatcggcg
     1081 tcctcttttg ccagctgctt gaacttgttg taactgattt ccgccacttc gcattcggtt
     1141 ttggcgcgaa tccaggcgct gcggttggcg gtcttgtcga acaggcccat ttcaccaaag
     1201 aaatcgcctt cgttcaggta agccatgatc atctcgcggc cctcatcgtc ctcgatgatg
     1261 accatcacgg tgcctttgat gatgtagtaa agcgcgtcgg aatcatcccc tgcatagatg
     1321 atcgtgctct tgctggggta tttgcggcgg tgacaatggg ccagaaagct ttctggatcg
     1381 ggaatcaaac tgttgggttt tatcgtcatt gcctgacctg gatgattgtt gttatcgaac
     1441 cgggttcgat cctgttaacc gggatggtgc ggatactatt tcattatggc tgcaaaagca
     1501 ggggctcgct actttatcta cccccgttgc gcccgggtgt ctacctgagc cggctattcc
     1561 ggccgccttc gctcccgcct gatgcgggtg atgcgttcca gtgcgctgtc cctggctggc
     1621 aacactaacc aaaacaacaa gattatcaaa tgattataga tgaagaggcg gtatcggact
     1681 gtgaacggtt gcagtgtttg ggcggagcta ttcacgaaag cctgtttagt gggcaaaaag
     1741 cagacgatcc gggggctggc gcggggcaat tttttacaca caatccggtt ggtttatgtt
     1801 atcttgcgcg ccattctgac catgggagtc agccatgaaa ggcactgttc gctgggcggg
     1861 tgacgtcaag tttgtagcgg aatccggttc cggtcacagc atcgtgatcg acggtccgcc
     1921 ggattatggt ggcaagaatc agggcgtgcg ccccatggaa ctgatgctgc tgggcgttgc
     1981 tggctgctcc tcgttcgacg tgattcatat cctgaagaag gcccgccagg atgtcactga
     2041 ctgcgtcacg gcggtggaag ccgaacgggt cgatgcggtg ccggccgtgt tcaagaaaat
     2101 ccacatgcac ttcgttgtga ccgggcgcaa tctcaaggat tcccaggtca agcgcgcggt
     2161 ggagttgtct gccgagaaat actgttcggc ctccatcatg ctgagcaagg gtggcgttga
     2221 gatcacccac agctacgagg tcaaagagac agagtgatgg gcaggcccgc tgcgttcggt
     2281 ggcctgcgcc acgtggccct gtttgtcgag aagttcgccg agtgcgaaca cttctatgtg
     2341 ggcctgctgg gtatgcaggt ggaatggcgg cccgaccccg ataacctgta tctgacgtcc
     2401 ggttgcgata atctggcctt gcaccgggca cccgccgcca tcgatcgcgg cagccgccag
     2461 aagctggatc acatcggctt cataatcgac atggaagcgg cagtcgatga ttggtacaca
     2521 tacctgaaat cgaatggtgt tgtgatgaga accgcgcccc ggtcccaccg cgatggcgca
     2581 cgtagttttt attgtgaaga cccggatggc accatggtgc agatgatatg gcatccgccc
     2641 attgccgggt tgaacatgag tcgggcgcag gcatgagtct gcgtccattg tgctgggcct
     2701 ctaccttgtg agatctgtgg agatgaaaaa tcccgaaacg ccggaaaaat tgaagttgca
     2761 tggtttcaac aacctgacca agtcactcag tttcaatatt tatgatatct gctatgcgaa
     2821 gacccctgag caccggcagg aatatcgtca gtacgttgat gagctgtaca acgccgagcg
     2881 tttgacccag atcctgacga cggtgaccga aatcgtcggc gccaacatcc tgaacatcgc
     2941 gcgccaggat tacgatccgg agggtgccag cgtgaccatg ctgattgccg agcatccgat
     3001 tgcgccgacg gaagatccgt cccgcgagga accgggtccg ctgaaggaat ccgtggtggc
     3061 gcacctggac aagagtcacg tgacggttca cacctatccg gaaacccacc cggacaacga
     3121 gaacggcatc agtactttcc gcgtggacat cgacgtgtcc acctgcggtg tcatttcgcc
     3181 gctgaaggct ctgaactacc tgatccacca gttcgattcc gatatcgtga cggtggatta
     3241 ccgcgtgcgt ggttttaccc gcgacgtgaa cggtcgcaag ctgtacatcg atcacgagat
     3301 caactcgatc cagaacttct tcctggatga aaccttggat ctgta
//
LOCUS       DASUEG010000324         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2154130, whole genome shotgun sequence.
ACCESSION   DASUEG010000324 DASUEG010000000
VERSION     DASUEG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154130"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="VFX11_06060"
     CDS             <1..1662
                     /locus_tag="VFX11_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938219.1"
                     /translation="DVSRARDILVAEIKTPLESRATEHTLLLALCHIRLGEVDQAKPL
                     LPYLKDDPALGTFTRFNLGSAFAQMGQLNKAGRYFTQVAETEATDENSLVLKDQATLA
                     LGVKLLKQEKWLEARDLLEHIRLYGPVANRGLLALGWTYFNSTDQTGALTPWLELAER
                     DLADPAVQEALLNIPYVYEQQGALQEALNRYREAHRIFQQQRNSLDNVKQEIQKPDWV
                     EKISPVSKEDTRNVMGEIPPFKLAADDPASRHLYLYFSSNEFQRIYRDYRELQRLYMV
                     LVHWQRQLPSYNQMIATHMERLEKLGPKSEKAIQNSRKFHAYANVKLDEYNARLAQIL
                     ASNDLAGTATVPQMTQKERLDAAEATLRALGEPDMYEEEWTKLRLLKGLLMWDLNATA
                     IDRRWEMTKDSIATENQLAELEQRIRAVEAARTARLDRFHGFEGRIVDLDKRMTGLQA
                     EIATALQHNRQYLQNVATGIVDQHQSQLDRMRAKALLSIARLQDRGYVQERERQGQQN
                     RQGLIMELQGQPATPDAPDADKPERANKGGEPDNLTDVIRNIFAD"
     gene            1759..2157
                     /locus_tag="VFX11_06065"
     CDS             1759..2157
                     /locus_tag="VFX11_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938220.1"
                     /translation="MPLPSKKHPAIRLVAWLSILGGLLTMGWSLYLLHTALEQQNWPS
                     AQVVRGGTGNDLHYRVGERHFQLEPAPDTAALPLTVVFYDPAKPDSYILKPSNYWWPL
                     YLSMAGLIVLYAGLHLQRERHRDVQQLGFE"
     gene            2185..2796
                     /locus_tag="VFX11_06070"
     CDS             2185..2796
                     /locus_tag="VFX11_06070"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX4938221.1"
                     /translation="MQQQLTDMLALQDRMNTKVHPQWREQGYEWYRAVWTECAELMDH
                     FGWKWWKKQTPDLPQLQLEIVDIWHFGMSILLVRAQDHTAVAHEIARQWTTQTSTGDF
                     RIAVEALAQDTLTTKSFSIPLFCQLMTLADFSLDQLYKQYTGKNVLNFFRQDHGYKEG
                     TYPRKWHGKDDNEHLAEVLDQLDAGAADFQQALYAALQARYPG"
     gene            2909..4402
                     /locus_tag="VFX11_06075"
     CDS             2909..4402
                     /locus_tag="VFX11_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX4938222.1"
                     /translation="MTLNRASYRPLSSFFAALTATRSTLFTTGALTALLACSNTFAAP
                     SLTIYNGNFAVVRDTLELPVNKGANRVQYQNITRLLEPDSVVLRSRKDDWKISIQEQN
                     FLANVVSEPLLLHYFEGKTIDFEVLRDQQPVIVPGKIIRSGYDDPGNSPIIEMEGKTR
                     FGLPGRPLFPALKDDTLLKPALQWQLESSQAGNAPLEIAYLTGGMNWKADYNIIATDN
                     SDKVSLNGWVTFTNNAGQDFSQATVKLMAGDVNKVEPAPQYRAKGMVMAAMEAPAPAV
                     TEKALDEFHLYSLPRKLDLRDGETKQVEFLNVTGVKAKKLFVYDGAAIPDHYRFQSPQ
                     DIRNDPNYGTQSNTKVWIMREFKNAKDNGLGVPLPKGRVRFYQQDEQQLEFIGENNID
                     HTPKDETVRLYTGNAFDIVGERERTDYQINTQEQWLRESFKITLKNRKQEKASVKVVE
                     HLYRWNNWALEKKSQDFEKKNADTIEFNVSLNHDEEKVVTYTARYSW"
     gene            4456..4869
                     /locus_tag="VFX11_06080"
     CDS             4456..4869
                     /locus_tag="VFX11_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011625360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX4938223.1"
                     /translation="MERISAQGGCLCGNIRYQIDAQPVDAGFCHCRLCQRATGAPVVA
                     WVTLPLEGFHYLKGSPAIFQSSSHSQREHCATCGTALVFRKSVAPVTLDVTLCSLDEP
                     TLIKPQYHIWLESRVDWLHISDSLPRHAGAGPDQH"
     gene            complement(4957..5118)
                     /locus_tag="VFX11_06085"
     CDS             complement(4957..5118)
                     /locus_tag="VFX11_06085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938224.1"
                     /translation="MGIALVPAHGLQLDDLIAKADSAMYEVKDHGRHHFSIYREPESP
                     AAFNSGAGP"
BASE COUNT         1186 a         1618 c         1455 g          859 t
ORIGIN      
        1 gatgtcagcc gtgcccgcga tatcctggtg gccgaaatca aaaccccgct ggaatcccgc
       61 gccaccgaac acacactgct gctggccctc tgccacatcc ggctggggga agtggatcag
      121 gccaaaccct tgctgcccta cctgaaagac gatccggcgc tgggcacctt cacccgtttc
      181 aacctgggtt ccgcttttgc gcagatgggt cagctgaaca aggccggtcg ctatttcaca
      241 caagtggcgg aaacggaagc caccgacgaa aacagcctgg tgctgaaaga tcaggccaca
      301 ctggcgctgg gcgtcaagct gctgaaacag gaaaagtggc tggaagcccg cgacctgctg
      361 gagcacatcc gcctgtacgg gccggtggcc aaccggggtc tgctggcctt gggctggacc
      421 tacttcaact ccaccgacca gaccggtgcc ctcacgccct ggctggagct ggcggaacgg
      481 gatctggcag accccgccgt gcaggaagcc ctgctcaaca ttccctatgt ctatgagcag
      541 cagggcgcgc tgcaggaagc gctcaaccgc taccgcgagg cgcaccgcat cttccagcag
      601 cagcgcaaca gcctggacaa cgtgaaacag gaaatccaga agccggactg ggtggagaag
      661 atcagcccgg tgtccaagga agatacccgc aacgtgatgg gagaaattcc gcccttcaag
      721 ctggcagcag acgatccggc ctcgcgccat ctgtacctgt atttttccag caacgaattc
      781 cagcgcattt accgcgacta ccgtgaactg cagcggctgt acatggtgct ggtgcactgg
      841 cagcggcagt tgcccagcta caaccagatg atcgctaccc atatggaacg cctggagaaa
      901 ctgggaccca aatcggaaaa agcgattcag aattcccgca aattccacgc ctatgcgaac
      961 gtgaagctgg acgagtacaa cgcccgtctg gcccagatcc tggccagcaa tgacctggcc
     1021 ggcaccgcca ccgtgccgca gatgacccag aaggaacggc tggacgcggc cgaagccacc
     1081 ctgcgcgcgc tgggtgaacc ggacatgtat gaagaggaat ggaccaagct gcgcctgctg
     1141 aaaggcctgc tgatgtggga tctgaacgcc accgccatcg accggcgctg ggaaatgacg
     1201 aaggacagca tcgccaccga aaatcagctg gcggaactgg agcagcgcat ccgtgcggtg
     1261 gaagcggcgc gcactgcccg cctcgaccgt ttccacggct ttgaaggccg catcgttgac
     1321 ctggacaagc gcatgacggg cctgcaagcc gagatcgcca ccgccctgca gcacaaccgc
     1381 cagtatctgc agaacgttgc caccggcatc gtcgatcagc atcagtccca gctggaccgc
     1441 atgcgcgcca aggcgctgct gtccatcgcc cgtctgcagg accgtggcta cgtgcaggaa
     1501 cgggaacggc agggccagca aaacaggcag ggcctgataa tggaactgca gggccagcct
     1561 gcaacaccgg acgcgccgga cgccgacaag cctgaacgcg cgaataaagg cggcgagccg
     1621 gacaacctca ctgacgtaat ccgcaacatc ttcgcagact gaacccgttc caactcccgg
     1681 cctgaatcca ttggaggcag tgccgggatg cgttagcata ccgctcagcc gtacacattc
     1741 ccgccacagg aacaacccat gccgcttcca tccaaaaaac atccggcaat ccgtctggtc
     1801 gcctggcttt ccatcctggg tggtctgctc acgatgggct ggagcctgta cctgctgcac
     1861 accgcactgg agcagcagaa ctggcccagc gcgcaggtgg ttcgcggtgg caccggcaat
     1921 gatctgcatt accgggtagg cgaacgtcat ttccagctgg aaccggcacc cgacaccgct
     1981 gccttgcccc tcactgttgt gttctacgac ccggccaagc cggacagcta tattctcaaa
     2041 ccatccaatt actggtggcc cctgtacctg agcatggcgg gcctgatcgt gctgtacgcc
     2101 ggcctgcacc tgcagcggga acgtcaccgc gacgtgcagc agctgggctt cgaatagatc
     2161 ttcaatcact tgcggggatg atccatgcaa caacaactga ccgacatgct ggccctgcag
     2221 gaccggatga acaccaaggt gcatccgcag tggcgggaac agggctatga atggtaccgc
     2281 gccgtatgga ccgagtgcgc cgaactgatg gaccacttcg gctggaaatg gtggaagaag
     2341 cagacgccgg atctgccgca actgcaactg gaaatcgtcg acatctggca cttcggcatg
     2401 agcatcctgc tggtgcgcgc gcaggatcac accgcggtcg cgcatgaaat tgccaggcag
     2461 tggaccacgc agaccagcac cggcgatttc cgcatcgcgg tggaagcact ggcgcaggac
     2521 acactgacga ccaagtcctt ttccatcccg ctgttctgcc agttgatgac cctggcggac
     2581 ttcagcctgg accagctgta caagcagtac accggcaaga acgtgctgaa ttttttccgt
     2641 caggatcacg gctacaagga aggcacctac ccgcgcaaat ggcacggcaa ggatgacaac
     2701 gagcacctgg ccgaggtgct ggaccagctc gacgccggtg ctgccgattt ccagcaggcg
     2761 ctgtatgcag cactgcaggc gcgttaccca ggctgaacgc acgccagaaa aagtcatcgc
     2821 taaagaaaca agcgcggcgg ggaacggcca ccctgtcgcg caggtccaac tggatgttca
     2881 tccccatcac gcccacagga cgccagacat gacgctgaat cgcgcatctt accgcccact
     2941 ttcttcattt tttgccgcac ttactgccac ccgctccacc ttgttcacca ccggcgcgct
     3001 gaccgcgcta ctcgcctgca gcaacacctt cgccgcaccc tcgctcacca tctacaacgg
     3061 caatttcgcc gtggtgcgcg acacactgga actgccggtg aacaaaggcg ccaaccgggt
     3121 gcagtaccag aacatcaccc gtctgctgga gcccgattcg gtggtgctgc gcagccgcaa
     3181 ggacgactgg aagatcagca ttcaggagca gaattttctc gccaatgtcg tcagcgaacc
     3241 gttgctgctg cattatttcg aaggcaagac catcgacttt gaagtactgc gcgaccagca
     3301 gcccgtgatc gtgcccggca agatcatccg cagcggttat gacgatcccg gcaacagccc
     3361 catcattgaa atggaaggca agacccgctt cggcctgccc gggcgcccgt tgtttcccgc
     3421 gctgaaagac gacacgctgc tcaaacccgc gctgcagtgg caactggaat cgtcacaggc
     3481 cggcaatgcg ccgctggaaa tcgcctacct caccggcggc atgaactgga aggcggatta
     3541 caacatcatc gccacggaca acagcgacaa ggtcagcctg aacggatggg tgacattcac
     3601 caacaacgcc ggccaggatt tcagccaggc cacggtgaaa ctgatggcgg gcgacgtcaa
     3661 caaggtggaa ccggcgccgc agtaccgcgc caagggtatg gtcatggccg ccatggaggc
     3721 ccccgcccct gccgtcaccg aaaaagcgct ggatgaattt cacctgtact cactgccacg
     3781 caaactggac ctgcgcgacg gcgaaaccaa gcaggttgaa tttctgaatg taaccggggt
     3841 gaaagcgaaa aaactgttcg tgtacgacgg cgccgccatt cccgatcact accgtttcca
     3901 gtcaccgcag gacatccgca acgatcccaa ctacggcacc cagagcaaca ccaaggtgtg
     3961 gatcatgcgc gaattcaaga atgcgaagga taacggcctg ggtgttccac tgccgaaagg
     4021 tcgcgtgcgc ttctaccagc aggacgaaca gcagctggaa ttcatcggcg agaacaatat
     4081 cgatcacaca cccaaggatg aaaccgtgcg gctgtacacc ggcaacgcct tcgacatcgt
     4141 cggcgagcgg gaacgtaccg attaccagat caacacccag gaacagtggc tgagggaatc
     4201 cttcaagatc acgttgaaga accgcaagca ggaaaaggcc agcgtgaagg tagtggaaca
     4261 tctgtaccgc tggaacaact gggccctgga aaagaaaagc caggatttcg aaaagaagaa
     4321 tgccgacacc attgagttca atgtcagcct gaatcacgat gaggagaagg tagtgactta
     4381 tacggcgcgg tattcctggt agtggctgga ctgcgccggc aaataccgca gcggattgaa
     4441 atccggagcg tctgaatgga aagaatatca gcccagggcg gttgcctctg tggcaacatc
     4501 cgctatcaga tcgatgccca gcccgtcgac gccggtttct gccactgccg gctctgccag
     4561 cgcgcgacgg gcgcgccagt ggtagcgtgg gtaacgctgc cactggaggg cttccactac
     4621 ctaaagggtt cacctgccat ctttcaatcc agttctcaca gtcagcgtga acattgcgcg
     4681 acctgcggta ctgcactggt gtttcgcaaa agcgtcgcgc cggtcaccct ggacgtcacg
     4741 ctctgctccc ttgacgaacc cacgctgatt aaacctcagt atcacatctg gctggaaagc
     4801 cgggtggact ggctccatat cagcgacagc ctgccacggc atgccggcgc cggacctgac
     4861 cagcactgac cccaccggac tgcctcattc ggtccccccc ggttcagggg ctgtggccaa
     4921 gccatcggcc ggctctgaca atctgtacac cgcgatttac ggcccggcac cactattgaa
     4981 ggcggcaggc gactccggtt cgcggtaaat ggaaaaatga tgacggccat ggtccttgac
     5041 ctcgtacatc gcgctgtcgg ccttggcgat cagatcgtcc agctgcaatc catgcgcggg
     5101 aaccagagca atgccgat
//
LOCUS       DASUEG010000325         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1032544, whole genome shotgun sequence.
ACCESSION   DASUEG010000325 DASUEG010000000
VERSION     DASUEG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032544"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFX11_06090"
     CDS             complement(<1..455)
                     /locus_tag="VFX11_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938225.1"
                     /translation="MIRVLICDDEPLARDRMRRMLEKIPATVCLGEAENGEELLEQVP
                     RLHPDLILLDIRMPGMDGLKAASRLSESDNPPAIVFCTAYDEHAIAAFKVNAIDYLLK
                     PVRQEDLEKALHKAARVNKAQMAAVRREMGEDTPVDADGKKEREYISAR"
     gene            complement(452..1573)
                     /locus_tag="VFX11_06095"
     CDS             complement(452..1573)
                     /locus_tag="VFX11_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4938226.1"
                     /translation="MASDPHKTPGEQEEAFFLPDLCARQPVFFLVLIAQLFALVIVLI
                     DSGLFPFDWQQLGLVSLFVQWVALSSAGLLCQMRKFLQTLPQERAVLISYLLIPVNTW
                     LVSLVSLYLLHPAEVTLGMLLFSEEVLRNVAVAAIIGGLVIRYFYLQAMLIARRQSEL
                     LHRIQALQSRIRPHFLFNSMNIIASLIETSPRQAETVVEDLSALFRASLNHVGNQVPL
                     AEEIDLCKRYLNIEQLRLGSRLQVQWEMRSVPAGVKIPLLTLQPLLENAILHGIQPLP
                     KGGLVEIIAGYSHGIFELTITNPFAAPTPGKVAEDERGNRMALQNTRNRLVALYGERA
                     KLTGYPEGNRYITRLSYPFAIDPPPAKANKTTTRRSVAP"
     gene            1673..>2952
                     /gene="argH"
                     /locus_tag="VFX11_06100"
     CDS             1673..>2952
                     /gene="argH"
                     /locus_tag="VFX11_06100"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX4938227.1"
                     /translation="MTDQAQDKLWGGRFTEATDAFVEAFTASVTFDKKMAEQDINGSI
                     AHATMLAAVGVLTDAEKQQIISGLEQIRSEIRAGTFEWSIKLEDVHMNIEARLTDRIG
                     ITGKKLHTGRSRNDQVATDIRLYMRDAIDAILKEITRLQTGLVNLAEREADTIMPGFT
                     HLQTAQPVTFGHHMLAWNEMLFRDYERLVDCRKRVNRMPLGSAALAGTTYPIDRTITA
                     QLLGFEGICENSLDGVSDRDFAIEFAAASALIMTHLSRYSEELVLWASAQFNFVDLPD
                     RFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLISLLTLMKSQCLAYNKDNQEDKEPLF
                     DTVETVLNCLRAYADMMPALQVKKDNMREAARRGFSTATDLADYLVKKGVAFRDSHEI
                     VGKAVGYGVAQKKDLADMSLAELQQFSPIITDDV"
BASE COUNT          597 a          903 c          893 g          559 t
ORIGIN      
        1 ctgcgggcac tgatgtactc gcgctccttt ttgccgtcgg cgtccaccgg ggtgtcctcg
       61 cccatttccc tgcgcacggc ggccatctgg gccttgttta cccgggcggc tttgtgcagg
      121 gccttttcca ggtcctcctg ccgcaccggc ttgagcaggt aatcgatggc gttgaccttg
      181 aacgcggcga tggcgtgctc gtcgtaggcg gtgcagaaca cgatggcggg gggattgtcg
      241 ctttcgctca agcgggaggc cgccttcagc ccgtccatgc cgggcatgcg gatgtccagc
      301 aggatcaggt cgggatgcag gcggggcacc tgttccagca actcctcacc gttttctgcc
      361 tcgcccaggc aaacggtggc gggaatcttt tccagcatgc gacgcatgcg gtcacgggcc
      421 aggggctcgt catcacagat cagcactcga atcatggggc cacacttctt cttgttgttg
      481 tcttattggc tttggctggc ggcgggtcga tcgcaaacgg atacgacagc cgcgtgatgt
      541 accgatttcc ttccgggtaa cccgttaatt tggcgcgctc accgtacagc gccaccagcc
      601 ggttacgggt attttgcagg gccatccggt tgccccgttc atcctcggcc accttccccg
      661 gagtgggggc ggcaaaggga ttggtgatcg tcagctcaaa gataccatga gaatatccgg
      721 caattatctc gacgagtcca ccttttggta acggctgtat accgtgaaga atcgcgttct
      781 ccagcagggg ctgcagggtc agcaggggaa tcttaacccc cgccggcacc gagcgcatct
      841 cccactgcac ctgcagacgg ctgcccagcc gcagctgctc gatgttcaga tagcgcttgc
      901 acaggtcaat ttcctccgcc agcgggacct ggttaccgac atggttaagg ctggcgcgga
      961 agagggctga caggtcttcc accacggtct cggcctgacg cgggctggtc tcgatcagac
     1021 tggcgatgat gttcatgctg ttgaacagaa agtgggggcg gatgcgggat tgcagggcct
     1081 ggatgcggtg cagcagctcg gactggcggc gggcgatcag catggcctgc aggtagaaat
     1141 agcggatcac cagcccgccg ataatggccg ccaccgccac gttgcgcagc acttcctcgc
     1201 tgaacagcag catgcccagg gtcacttcgg ccgggtgcag caggtacagg ctgaccaggc
     1261 tcaccagcca ggtattcacc ggaatcagca ggtaactgat cagcaccgcc cgctcctggg
     1321 gcaaggtttg caggaacttg cgcatctggc acagcaggcc ggcgctggac agggccaccc
     1381 actgcacgaa cagcgacacc agccccagct gctgccagtc gaaggggaac aggccactgt
     1441 cgatcagcac gatgaccagg gcgaacagct gggcaatcag caccaggaaa aacaccggct
     1501 ggcgcgcaca cagatccggc aggaagaacg cctcttcctg ctcgccggga gtcttgtggg
     1561 ggtccgatgc catgggaatc cgtagtgggt tataatccgc gccttgtttg agtatatgcc
     1621 aaccacccaa acccgttttt gttccagccc gcatttttca gtggaatcca gcatgacaga
     1681 ccaagcccag gacaaacttt ggggtggccg cttcaccgaa gccaccgacg ccttcgtgga
     1741 agccttcacc gcctccgtca ccttcgacaa gaaaatggcg gagcaggaca tcaacggctc
     1801 catcgcccat gccaccatgc tggccgccgt gggcgtgctg accgatgcgg aaaaacagca
     1861 gatcatcagc ggcctggaac agatccgcag cgaaatccgc gccggcactt tcgagtggtc
     1921 gatcaagctg gaagacgtgc acatgaatat cgaggcgcgc ctgaccgacc gcatcggcat
     1981 caccggcaag aaactgcaca ccggccgctc ccgcaacgac caggtggcca ccgacatccg
     2041 cctgtatatg cgcgatgcca tcgacgcgat cctgaaagaa atcacccgcc tgcagaccgg
     2101 tctggtgaat ctggccgagc gcgaagccga taccatcatg cccggcttca cccatctgca
     2161 gacggcacag ccggtcacct tcggccacca catgctggcc tggaacgaaa tgctgttccg
     2221 cgattacgaa cgcctggtgg attgccgcaa gcgcgtaaac cggatgccgc tggggtctgc
     2281 tgctttggca ggaaccacct accccatcga ccgcaccatc acggcgcaac tgctgggctt
     2341 tgaaggcatc tgcgagaact cgctggacgg cgtgagcgac cgtgacttcg ccattgaatt
     2401 tgcggcggcg tcggcactga tcatgacgca cctgtcgcgc tattccgagg agctggtgct
     2461 gtgggcttcg gcgcagttca atttcgtcga tctgccggac cggttctgca ccggctcgtc
     2521 gatcatgccg cagaagaaaa atcccgacgt gccggaactg gtgcgcggca agactggccg
     2581 cgtcaatggc cacctgattt cactgctgac gctgatgaaa tcccagtgtc tggcgtacaa
     2641 caaggacaat caggaagaca aggaaccgct gttcgacacc gtggaaaccg tgctgaactg
     2701 tctgcgtgcc tatgccgaca tgatgccggc gctgcaggtg aagaaagaca acatgcgcga
     2761 agcggcacga cgcggctttt ccactgccac cgacctggcg gactatctgg tgaagaaagg
     2821 cgtggcgttc cgcgattccc acgagatcgt cggcaaggca gtaggttatg gcgtggcgca
     2881 gaagaaggat ctggcggaca tgtcgctggc ggaattgcag cagttcagtc cgatcattac
     2941 cgatgatgtt tt
//
LOCUS       DASUEG010000326         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_357155, whole genome shotgun sequence.
ACCESSION   DASUEG010000326 DASUEG010000000
VERSION     DASUEG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357155"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFX11_06105"
     CDS             complement(<1..1280)
                     /locus_tag="VFX11_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4938228.1"
                     /translation="MLSQIAVFKPARAFSAQGIALRMALPLLAAALVLTPGLLLIHWL
                     QAESSLYAWLLVSGVALGTGLAIAVLLGLQLHDEYLHTHDDLAQALRRRVVNRQYDQP
                     LPVPASAATPELVAEINRLLHWLGQQQQDQADEKALLELRAQEQDVALQQEINARRSA
                     QEKLQKLARELDNSNRELAESLRLSQSADKAKAEFLANMSHEIRTPLNAVIGVASLLD
                     RSSLSERQRQQVGMIFESGKSLLRTLNDIMDYVRIEAGEIALDAFPFNLADTLGAVFL
                     QHRELAQARGLEYSLRYGDPLPKLFVGDGARIQQLVHSLIDNAIKFTHDGSVQVHISG
                     YALHSARYLMRIEVRDTGIGISAEKRDDLFNIFTQGDNSITRAYGGSGLGLAICSRLV
                     KLMEGTLGMDSEPGQGSLFWVELPLRLYRDITVA"
     gene            complement(1359..2492)
                     /locus_tag="VFX11_06110"
     CDS             complement(1359..2492)
                     /locus_tag="VFX11_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4938229.1"
                     /translation="MSQAGAFPFIVRLNRVDCLTLSSVVTTALATAMTLDQHFHLAMA
                     LLFVAMGCDGLDGLLARKWGLARNFGRYLDGFMDVLIYLVSPALLAYQSGFNGLWGLL
                     LMLTIGCGCVRLAVFNEIGNIEDAGRSRYLGMPVFWSLFILAGYQLLLLQLPLLVCNS
                     LLALALLLFSVLMVSRQPFFKFTSLLQILALTGGGALLFLLLDLRQRGIDFTHWPWST
                     VFWLQLPVVIGGVLHMVVVTRDWLPALKIPVQRRWFGANKTLRGFVVMPLLSALGGWC
                     MAPSGEWVLTGALAGLAYVLAELPNSFIKRRMGVPPGEVPRRFRLLFVAVDQLDSGLG
                     IALLYALLGYGWDVVVLFALSFPVTALVVKQILFRLRLKRTAA"
     gene            complement(2536..3669)
                     /locus_tag="VFX11_06115"
     CDS             complement(2536..3669)
                     /locus_tag="VFX11_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938230.1"
                     /translation="MPSGTGHACGPFDPDQALPPPFAAVAQSVYADDPYWLGEDAEAL
                     QRRFSRANDFFQQGRAWLGVADGVVNSAARLAGFVHPQQVVDGKPAAWFGFWETADLL
                     APNKKLFDRLEQWAREQGASALYGPIDFNTFGRNRLRLNDFDQSCFPGEPYNPDYYPR
                     LLTQLGFGIERRYQSRFHLQAPVLTDELQPRLQASRAALQEQFRITPLTPDYWLDHLD
                     DLYPLVDTIFRDNFAYTPVSRAQFGASCGETFARTFCPRTSVLATTPDGDVAGFFLCF
                     PDYSPLLRHANPQRLAPEKIRYDVHAQLLPQPTLALAKTGGVHPRFRQSGLFNWMGLE
                     LVVKVGMHYDLLCGALMREDNPSLRFGMICPLQRDYGLFGKAL"
     gene            complement(3669..>3858)
                     /locus_tag="VFX11_06120"
     CDS             complement(3669..>3858)
                     /locus_tag="VFX11_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938231.1"
                     /translation="GTDIDPEQWRRGYQLSLRDLLINRVPLYLMAHTFRHYKFMDRVQ
                     RSLRRLLQLEAQRVRENA"
BASE COUNT          834 a         1234 c         1157 g          633 t
ORIGIN      
        1 tccgccaccg taatgtcccg gtaaagccgc aacggcagtt ccacccagaa cagcgaaccc
       61 tgccccggtt cgctgtccat tcccagcgtt ccttccatca gcttcaccag ccgtgaacag
      121 atcgccagcc ccaggccgct gccgccgtag gcacgggtga tggagttgtc gccctgggtg
      181 aagatgttga acagatcgtc gcgtttttcc gccgagatac cgatgccggt gtcgcgcact
      241 tcgatgcgca tcaggtaacg ggcgctgtgc agcgcatagc cgctgatgtg gacctgcacg
      301 ctgccgtcat gggtgaactt gatggcgttg tcgatcaggc tgtggaccag ttgctggatg
      361 cgcgcaccgt cacccacgaa cagcttgggc agcggatcgc cgtaacgcag cgaatattcc
      421 agcccccgcg cctgcgccag ttcgcgatgc tgcaggaaca cggcgcccag cgtgtcggcc
      481 agattgaagg gaaacgcgtc cagcgcgatt tcgccggctt cgatgcgcac gtaatccatg
      541 atgtcgttca gtgtgcgcag cagggatttg ccggattcga aaatcatgcc cacctgctgg
      601 cgctggcgct cggacaacga tgagcggtcg agcaggctgg ccacgccgat caccgcattc
      661 agcggcgtgc ggatttcatg gctcatgttg gcgaggaatt ccgccttggc cttgtcggcg
      721 ctctggctca ggcgcaggct ttccgccagt tcgcggttgc tgttgtccag ttcgcgggcc
      781 agtttctgca gtttttcctg ggcggaacga cgggcgttga tttcctgctg cagcgcgacg
      841 tcctgttcct gggcgcgcag ttccagcagg gccttttcgt ctgcctgatc ctgttgctgc
      901 tggcccagcc agtgcagcag gcggttgatc tcggccacca gttccggcgt ggcggcgctg
      961 gcgggcaccg gcaggggctg gtcgtactgg cggttcacca cgcgccgccg cagggcctgc
     1021 gccagatcgt cgtgggtgtg aaggtattca tcgtgcagtt gcagtcccag caacaccgca
     1081 atggccagcc cggtgcccag cgcgacacca ctcaccagca gccaggcata cagcgacgat
     1141 tccgcctgca gccagtgaat cagcagcaag ccgggcgtca gcaccagggc ggcagccagt
     1201 aacggcaagg ccatgcgcag cgcaatgccc tgggccgaga atgcccgagc cggcttgaaa
     1261 acggcgattt gagataacac gctagacagc actttggtta gcaacagacg cggcaccgat
     1321 cgttcccctc cgaaaggtgc cttaactata gatgattttc aggcagcagt gcgtttcagg
     1381 cgcaagcgga acaggatctg cttcacaacc agtgccgtga cggggaaact gagggcgaac
     1441 agcaccacca catcccagcc ataacccagc agcgcgtaca gcagggcgat gccaaggccg
     1501 gaatccagct ggtccaccgc cacgaacagc aggcggaaac ggcggggcac ttcgcccggc
     1561 ggcacgccca tgcggcgctt gatgaacgag ttgggcagct ccgccagcac ataggccagc
     1621 cccgccagcg cgccggtgag cacccattca cccgaaggcg ccatgcacca gccacccagt
     1681 gcagacagca gtggcatcac cacaaaaccg cgcagcgtct tgttggcgcc gaaccagcgc
     1741 cgctgcaccg gaattttcag cgccggcagc cagtcgcggg tgaccaccac catgtgcagc
     1801 acgccgccga tcaccaccgg caactgcagc cagaatacgg tggaccaggg ccagtgggtg
     1861 aaatcgatgc cgcgctggcg caggtccagc agcaggaaca gcaacgcacc gccgccggtg
     1921 agggccagaa tctgcagcag cgaagtgaac ttgaagaacg gctggcgcga caccatcagc
     1981 accgagaaca gcaacagcgc cagcgccagc aggctgttgc acaccagcag cggcagctgc
     2041 agcaacagca gctggtatcc ggccagaatg aacagactcc agaacaccgg catgcccaga
     2101 tagcgggaac ggccggcgtc ctcgatattg ccgatctcgt tgaagaccgc cagccgcacg
     2161 cagccgcaac cgatggtcag catcagcagc aatccccaga ggccgttgaa accggactgg
     2221 taggccagca gggccgggct caccagatag atcagcacat ccatgaagcc atccagataa
     2281 cggccgaaat tgcgcgccag tccccacttg cgcgccagca ggccgtccag cccgtcgcac
     2341 cccatggcga cgaacaacaa ggccattgcc agatgaaaat gctgatccag cgtcatggcc
     2401 gtggccagcg ccgtggtcac cacgctggac agcgtcaggc aatcgacgcg gttcaggcgg
     2461 acaatgaaag gaaatgctcc cgcttgcgac atcttttgat tccccaataa cgacaaaaaa
     2521 caactacaaa aacgactaca aggcttttcc aaacaaacca taatcccgct gcagcggaca
     2581 gatcatgccg aaccgcagcg acggattgtc ttcccgcatc agcgcgccac acagcaggtc
     2641 gtagtgcatg cccaccttca ccaccagttc cagccccatc cagttgaaca gccccgactg
     2701 gcggaaacgt ggatgcacac caccggtctt ggcgagcgcc agggtcggtt gcggcaacag
     2761 ctgtgcgtgc acgtcatagc ggattttttc cggcgccaga cgctgcggat tggcatgacg
     2821 cagcagcggc gaataatcgg gaaagcacag gaaaaatcct gccacatcac catcgggcgt
     2881 ggtggccagc accgaggtgc gcggacagaa cgtgcgtgcg aatgtttcac cgcaactggc
     2941 accaaactgc gcgcgcgaaa ccggcgtgta ggcgaagttg tcgcgaaaaa tcgtatccac
     3001 cagcgggtaa agatcatcaa gatgatcgag ccagtaatcc ggcgtcagtg gcgtgatccg
     3061 gaactgctcc tgcagtgcgg cgcgggacgc ctgcagacgc ggttgcagtt catccgtcag
     3121 tactggcgcc tgcagatgaa accggctctg gtaacgccgt tcaatgccga aaccgagttg
     3181 ggtgagcagg cgcggataat aatccggatt gtagggttca ccgggaaaac aggattgatc
     3241 gaaatcgttc agccgcagac gattgcgacc gaaggtgttg aaatcgatag ggccatacag
     3301 cgcactggcg ccctgttccc gcgcccactg ttccagccgg tcgaacagct ttttgttcgg
     3361 cgccagcaga tcggcggttt cccagaagcc aaaccaggct gccggtttgc catcgacaac
     3421 ctgctgcgga tgtacgaatc ctgccagccg cgccgcgctg ttcacaacgc cgtccgccac
     3481 acccagccac gcacgcccct gctggaaaaa atcattggcg cgggagaaac gccgctgcaa
     3541 ggcttcagca tcttcaccca gccaataggg atcgtccgca taaactgact gtgcgacagc
     3601 agcgaacggt ggcggcagcg cctggtcggg atcaaacggc ccgcaggcgt gcccggttcc
     3661 ggaaggcatc aggcattctc cctcacccgc tgcgcttcca gttgcagaag acggcgcagc
     3721 gaacgctgca cccggtccat gaatttgtaa tgccggaacg tgtgtgccat caggtacagg
     3781 ggcacgcggt tgatcagcag atcgcgcagg ctgagctgat agccgcgccg ccactgttcc
     3841 ggatcgatat cggtgccg
//
LOCUS       DASUEG010000327         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_128946, whole genome shotgun sequence.
ACCESSION   DASUEG010000327 DASUEG010000000
VERSION     DASUEG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128946"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFX11_06125"
     CDS             <1..205
                     /locus_tag="VFX11_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938232.1"
                     /translation="ASKALSQSVGADFLLIGEISNVIRSQPQQKEVWYRLSLKLVDER
                     SGQFLWQERREFLKSQKKVIYGI"
     gene            complement(236..535)
                     /locus_tag="VFX11_06130"
     CDS             complement(236..535)
                     /locus_tag="VFX11_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938233.1"
                     /translation="MNSQSTNSQFTQGQNNASLTLLQLAAGCALLVFGLRVLAPLPER
                     SSEWPAPVAVVPLSADRELPNCEGNRATVEGWSVQTAACVTGGDQAAEWTPPLSI"
     gene            720..1418
                     /gene="pdsR"
                     /locus_tag="VFX11_06135"
     CDS             720..1418
                     /gene="pdsR"
                     /locus_tag="VFX11_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEX4938234.1"
                     /translation="MAKHIALIEDEAAIRENYKAFLQRQGYQVSTYDSRPSALKAFHQ
                     SLPDMAVIDIGLQDEIEGGFDLCRELRQLAPQLPIIFLTARDSELDAISGLRLGADDY
                     LTKDISLPHLAARIIALFRRLDILSKPSTLANRLERQALKLDLDRMYATWQEQNLDLT
                     LTEFWILHALAKHPGHVKNRQQLMDAANVVLDDNTITSHIKRIRKKFNQIDASFDAIQ
                     TVYGMGYRWHIPDA"
     gene            1422..>2328
                     /locus_tag="VFX11_06140"
     CDS             1422..>2328
                     /locus_tag="VFX11_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938235.1"
                     /translation="MGLRSQLLLVSLCTLLLPWAGCQYVQEMEGVLRENQSHAMIGQT
                     ALLARMLNQTVDLPAPVKGSLFYTPVRYQSVEMDGYGDDWQGHHADTLENAAASMDLR
                     LQKGLHGNDLYLFLQLHDTRLIYHHPGQSFRRSDHVRVRTGAGKQELQWLFFTSGPGQ
                     LQVYEKRTGYKRLRARPEIDAWWQETGTGYQLEIRIPHKLIRTHLDIQVYDRDPYEKK
                     TRLALSTRRGGDASVWVKPVAELLPALQPWRTHDTDITLVDPRGWPLAPQKNWLNDDS
                     PLDSNVTLDQGLIDQTISRFYRYLVE"
BASE COUNT          481 a          785 c          671 g          391 t
ORIGIN      
        1 cgcctcgaag gcccttagcc agtctgtggg tgctgacttc ctgctgatcg gtgaaatcag
       61 caacgtgatt cgctcgcaac cgcagcagaa ggaagtctgg taccggctat cgctgaagct
      121 ggtggacgag cgcagcggcc agttcctgtg gcaggaacgg cgcgagtttc tgaagagcca
      181 gaagaaagtc atctacggca tctgataccc gttttcagac gttgacatac gcctctcaga
      241 tcgacagcgg cggggtccac tccgccgcct ggtccccgcc agtgacgcac gccgcagttt
      301 gaacgctcca gccttccacc gtcgctctgt tcccttcaca attgggcaat tcacgatcag
      361 ctgacagtgg aacaaccgcc acgggcgcgg gccactcgga cgagcgctcc ggcagcgggg
      421 ccagcacccg caatccgaac accagcagcg cacagccagc cgccagttgc agcagggtca
      481 ggctggcatt gttctgtccc tgggtgaatt ggctgttcgt ggattgactg ttcatggcag
      541 acctcttccg ggtggggatg aataccctgc cattgcaccg gctttttctg gcggcaaacc
      601 gccgcgatcg gggcagactg ccgctgaatc ctgatcattt gtggcagtgc actttctgca
      661 cgccgcacat gatatacact gaccccattg cgcatccgca ccagccagcc acgagattta
      721 tggccaaaca cattgcactg atcgaagacg aagcggcgat ccgcgaaaac tacaaggcct
      781 tcctgcagcg tcagggttac caggtcagca cctacgattc gcggccgtcg gccctcaagg
      841 ccttccacca gtcgctgccg gacatggcgg tgatcgacat cggcctgcag gatgaaatcg
      901 aaggcggctt cgacctgtgc cgggaactgc gccagctggc accgcaactg ccgatcatct
      961 tcctcaccgc ccgcgacagc gaactggatg cgatttccgg cctgcgcctg ggggccgacg
     1021 actacctgac caaggacatc agcctgcccc acctggcggc ccgcatcatt gccctgttcc
     1081 gccggctcga cattctcagc aagccatcga ccctggccaa caggctggaa cggcaggcac
     1141 tgaagctgga cctggaccgc atgtacgcca cctggcagga gcagaacctg gacctgaccc
     1201 tgaccgaatt ctggattctg cacgcactgg ccaaacaccc cggccacgtg aaaaaccgcc
     1261 agcagctgat ggacgccgcc aacgtggtgc tggacgacaa caccatcacg tcccacatca
     1321 agcgcatccg caagaagttc aaccagatcg acgccagctt cgacgccatc cagaccgtgt
     1381 atggcatggg ttaccgctgg cacatcccgg acgcctgatt cgtggggctg cgttcacaac
     1441 tgttgctggt ttcactctgt accctgctgc tgccctgggc aggctgccag tacgtgcagg
     1501 aaatggaagg cgtgctgcgg gagaaccaga gccacgccat gatcggccag actgccctgc
     1561 tggcccgcat gctgaaccag accgtggacc tgccggcgcc ggtgaaaggc agcctgttct
     1621 acaccccggt gcgctaccag agcgtggaaa tggacggcta cggcgacgac tggcagggcc
     1681 accacgccga cacgctggaa aatgccgccg cctccatgga cctgcgcctg caaaaggggc
     1741 tgcacggcaa cgacctgtac ctgtttctgc agctgcacga cacccgcctg atctaccacc
     1801 accccggcca gagcttccgc cgcagcgatc atgtgcgcgt gcgcacggga gccggcaagc
     1861 aggaattgca gtggctgttc ttcaccagtg gcccgggaca attgcaggtt tatgaaaaac
     1921 gtaccggcta caagcggctg cgggcgcgcc ccgagatcga cgcctggtgg caggagaccg
     1981 gcaccggcta ccagctggaa atccgcattc cgcacaagct gatccgcacc catctggaca
     2041 tccaggttta tgaccgcgat ccctacgaga agaaaacccg gctggccctg tccacacggc
     2101 ggggcggcga cgcttcggtg tgggtgaaac cggtggcaga attgctgccg gccctgcagc
     2161 cgtggcgcac ccacgacacc gacatcaccc tggtggaccc ccgcggctgg cccctggcac
     2221 cccagaaaaa ctggctcaac gacgacagcc cgctggacag caacgtcacc ctcgaccagg
     2281 gcctgatcga ccagaccatc agccgcttct accgctacct ggtggaac
//
LOCUS       DASUEG010000328         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1848052, whole genome shotgun sequence.
ACCESSION   DASUEG010000328 DASUEG010000000
VERSION     DASUEG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848052"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFX11_06145"
     CDS             complement(<1..900)
                     /locus_tag="VFX11_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4938236.1"
                     /translation="MMMRSRTFRDVITTSFWALVNALSSRLLNLLTMLLAARALTAGE
                     YGSLALMLNSAQAWSLLIGAGFSLTLSRFVASYRESQASRTPGLVVTLLLTALLLTVL
                     VACSLMTFAEPLSRQFFAGSLLSDDVVVVGMWTFLLALVTVVQGALSGFEAFRQNALI
                     GLGCGLLVLLLVFAGPATNQRWVLGSLLAGNVLTLLLSGVLLLLRVKTLYQPGNRLVD
                     AAHLGPVLRFGLPALLGGVIVTQSQWHANVEVASTLGGLQKMAGLSVALQWYAIVQFV
                     PGVIANSMLPMLARTERDPGTARR"
     gene            complement(901..2136)
                     /locus_tag="VFX11_06150"
     CDS             complement(901..2136)
                     /locus_tag="VFX11_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938237.1"
                     /translation="MMLYLGLAPIYWLPGIPFEMLAQFKTGLFVLAAGLLFLTGVVGE
                     RAHFPPGLFGPAGLGLSLLAITGGIFQSEIGPAVTAVKDYLLVFSTLWMFYLLVQMQM
                     DVEKILLGASLLVAIACVPVFASKYLGGPDFAGPSQFVADHLWISGFSSVRTGWSNGI
                     ALYVAPLLLVLVRPMRSGFLLKALSGLAVCAIILAQITVAGRAGMVASLLVLLFAFST
                     RGYRTVLLVIAVAGIVFVALNPNTVASHLRITRDPATQQGADDIDTISAGRLQGDVVG
                     IQRALESPLIGHGLRNAVVGDGEIHNLWIRLANESGLFAPIVLLVIVAGVSRRALMMV
                     RQAGFDRRQRFRSNVCLGAIAGGVFMSMVEPRILLGTFQISVMWWAFAGLVCASALRR
                     PVREQPVVAATQPPLMNFV"
BASE COUNT          584 a          737 c          585 g          337 t
ORIGIN      
        1 tcttcgcgcc gtgccaggat cacgctcggt tcgtgcaagc atcggcaaca tggaattggc
       61 gatcacgccg ggaacgaatt gcacgatggc ataccactgc aacgcaacgc tgagacccgc
      121 cattttttgc agcccgccaa gggtagatgc cacttccaca ttggcatgcc attgcgactg
      181 ggtgacgatt actccaccca acagtgcggg caaaccaaaa cgcagaaccg gtcccagatg
      241 agccgcatca accagccgat ttccaggttg atacaacgtc ttcacacgca gcaacagcag
      301 cacgccggaa agcagcaacg tcaacacatt gccggcaagc agtgatccca gcacccagcg
      361 ttgattcgtg gcaggcccgg caaacaccag cagcagcacc aacaacccgc aacccaaacc
      421 aatcagcgcg ttctggcgaa aggcttcaaa gcccgacagc gcaccctgca ccactgtgac
      481 cagtgccagc aggaacgtcc acatgcccac cacaacgacg tcgtcactca gcaggctgcc
      541 ggcaaaaaac tggcgggaaa gcggctccgc aaacgtcatc agggaacagg caaccagcac
      601 cgtcagcagc agcgctgtca acaacagcgt gacgaccagc ccgggcgtgc gcgaagcctg
      661 gctttcccgg taactagcca caaagcgcga cagcgtcagg ctgaagccgg ctccgatcaa
      721 caatgaccac gcctgcgccg aattcagcat aagtgcgagc gatccgtact ccccggccgt
      781 caacgcgcgc gcggccagca gcatggtcaa caggttcagc aagcgcgacg acagggcatt
      841 caccaaggcc cagaaactgg tggtgattac atcgcggaac gtacggctcc tcatcatcat
      901 tcagacaaaa ttcatcaacg gcggctgcgt cgctgcaaca acaggttgct cacgcaccgg
      961 tcggcgcagt gcagacgcac agaccaggcc ggcaaatgcc caccacatta ccgaaatctg
     1021 gaaagtgccg agcaagatgc gtggctcaac catcgacatg aacaccccgc ccgcaatggc
     1081 tcccagacag acattgctgc ggaagcgctg ccgccggtcg aaccctgctt gccgcaccat
     1141 catcaatgcg cgccggctca cgccggccac aataacgagt aacacgatag gtgcgaacag
     1201 gcccgattcg ttggccagcc tgatccacag attgtgaatt tcgccgtcgc caacaacggc
     1261 attccgcaaa ccatgaccaa tcagaggact ctccaatgcc cgctgaatgc ctacaacatc
     1321 accctgcagg cgcccggcgc tgatggtgtc gatgtcatcc gcgccctgct gcgtggcggg
     1381 gtcacgggtg atgcgcaagt gggaagccac ggtgttcggg ttcagcgcca cgaacacaat
     1441 gccagcgact gcgatcacca gcaacactgt acgatatccc cgggtggaaa atgcaaacag
     1501 caacaccaga agcgacgcca ccatccctgc cctgcccgcc actgtgatct gcgccaggat
     1561 aatggcgcac accgccaatc cagatagcgc cttgagtaga aacccacttc gcatgggccg
     1621 gaccagaaca agcaggagcg gcgcgacata taacgcaatt ccattggacc agccggtgcg
     1681 cacgctgctg aaaccactga tccagagatg atctgcaaca aactgcgaag gccccgcgaa
     1741 atcgggtccg cccagatatt tgcttgcaaa caccggcacg caggcaatcg cgaccagcag
     1801 actggcgccc agcaggattt tttcgacatc catctgcatc tgcaccagca ggtagaacat
     1861 ccacagtgtg gaaaatacca gcaggtaatc cttgacagct gtcacggccg gcccgatttc
     1921 agactggaat atgccgccgg taattgccag cagagaaagc ccgaggcccg caggaccaaa
     1981 caggccagga ggaaaatgag ccctttcgcc aacaacgccc gtcaggaaca gcaggcctgc
     2041 tgccagcacg aataatccgg tcttgaattg ggccagcatt tcaaacggaa tcccgggcaa
     2101 ccaataaatt ggtgccaacc ccaggtacag catcaacaca aagggcacga ctgctttcat
     2161 gggctactgc attcccgcat tgccgccaac atgcagcggc ttgacggagc gggtcttctc
     2221 gctcacgaga aagtagccgc ccg
//
LOCUS       DASUEG010000329         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1061690, whole genome shotgun sequence.
ACCESSION   DASUEG010000329 DASUEG010000000
VERSION     DASUEG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061690"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFX11_06155"
     CDS             <1..1180
                     /locus_tag="VFX11_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938238.1"
                     /translation="THEAQISVGDRTFRLTANPILQDNRRVGTVVEWKDRTAELRIEA
                     EIDRIVEQASAGDFNTHLNLDGRTGFFLSLSRGLNRMIDVTRNALTDVTKIMERVAQG
                     DLTQKVEKGYTGLFGQLKDDVNSTIARLEEVIGNIMESANTISSGAAEVAVGMNDLSS
                     RTEQQAASLEETASSMDQMTSAVKASNEHAQNATRYAQEAERAAVQGGEVVQQAIDSM
                     QSINAASRRIADIIGVIDGIAFQTNLLALNAAVEAARAGEQGRGFAVVAAEVRNLAQR
                     SGNAAREIKTLIKDSLEKVDNGSRLVNQSGDTLTSIVKAVEEVAVFVKTIANASREQT
                     SGITQVNTAVAQMDQMTQQNSALVEEVTAAGVTMADQAQKMSAELSFFNVRKNSGAAH
                     "
     gene            1186..>2673
                     /locus_tag="VFX11_06160"
     CDS             1186..>2673
                     /locus_tag="VFX11_06160"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4938239.1"
                     /translation="MSLPATQPSLPLHWQILLVAVAYFALGRIGLMLAIPPGYASPLW
                     PPAGMALAALLLWGPRCWPGVLLGSFMVNLSIGLNTIGLPQVVESTAVAFLISAGAAA
                     QAIGSRWLILRVTQPPWRFDHPRQIFAVNLLGGLAGSSINATLGNIVLMAFGLQDMHS
                     VGYAWFNWWVGDTIGVFFFTSLLLLIATPGPYITGLRKNWVAMPMLLMFLLVMLLFIT
                     TQQYERNRILEQLRNESDDIMHELSGQIDTVLGILASMQRFHSASESVTRDEFTAFTA
                     GILATTPSIAALEWIPEVSLAQREAFEARLQAEGFAQFRFTEISDNGELVTAGVRPFY
                     YPVIYVEPLQRNLKALGLDLGSHPARLDAIGRALASNQFAATRALHLVQDSNSSSPAV
                     LIFEPLQHHASGERSLLLEIVRFSALVDTVMPIAHRKAISFTIMGQDMRQVLINQSGE
                     TGHAQPLVEPKGTRVVHDLKIADQTWKVIFPLDKRYLDHNSTWYVW"
BASE COUNT          573 a          834 c          765 g          501 t
ORIGIN      
        1 cactcacgaa gcccagatca gtgtggggga tcgtaccttc cgcctgaccg ccaatccgat
       61 cctgcaggac aaccggcgcg tcggcaccgt ggtggaatgg aaagatcgca cggcagaact
      121 gcgcatcgaa gcggaaatcg accgcattgt cgaacaggcg tcagcgggcg atttcaatac
      181 ccatctgaac ctcgacggtc gcaccggatt tttcctgtcg ctcagccgcg ggctcaatcg
      241 catgatcgac gtcacccgca atgcactcac cgacgtgacg aagatcatgg aacgggtggc
      301 gcagggagac ctgacccaga aagtggaaaa gggatacacc ggactgttcg gccagttaaa
      361 ggacgacgta aacagcacca ttgcgcggct ggaagaagtg attggcaaca tcatggaatc
      421 cgccaacact atttccagcg gtgcagcgga agtcgccgtc ggcatgaacg atctcagttc
      481 ccgcacggaa caacaggctg cgtcgctgga agagactgcg tccagcatgg accagatgac
      541 gtcagccgta aaagccagca acgaacacgc ccagaatgcc acccgctatg cgcaggaggc
      601 agagcgcgcg gcagtgcaag gtggcgaagt cgttcagcag gccattgact cgatgcaatc
      661 catcaacgca gccagccgcc gcattgctga catcatcggt gtcatcgatg gcatcgcgtt
      721 ccaaaccaat cttctggcat tgaacgccgc tgtcgaagcc gcccgcgcgg gcgagcaggg
      781 acgcggtttt gccgtggtgg ctgccgaagt gcgcaatctg gcccagcgtt ctggcaatgc
      841 cgcccgcgag atcaaaacgc tgatcaagga cagcctggaa aaagtcgaca acggctcgcg
      901 cctagttaac caatctggcg ataccctgac gagtatcgtg aaggctgtgg aggaagtagc
      961 agtctttgtc aaaacaatag ccaatgcatc gcgggaacaa accagcggaa tcacccaggt
     1021 caatactgct gtcgcgcaga tggaccagat gactcagcag aattcagcgt tggtggagga
     1081 agtcacggcc gccggggtca cgatggcgga tcaggcgcag aaaatgagcg ccgagctttc
     1141 attctttaat gtaagaaaaa acagcggagc tgcacactga gcaacgtgtc actgcctgca
     1201 acccagccct cactgccgct gcactggcaa atcctgctgg tggccgtagc ctatttcgcg
     1261 ctggggcgga tcggtctgat gctggcgatt ccgcccggtt atgcatcgcc cctgtggcca
     1321 cccgccggca tggcgctggc ggcgctgctg ctgtggggtc cgcgctgctg gcccggtgtt
     1381 ctgctgggtt cgttcatggt gaacctctcc atcggtctga acaccatcgg cctgccacaa
     1441 gtggtggaat ccacggccgt cgcgttcctg atcagcgccg gtgcagccgc ccaggccatc
     1501 ggttcgcgct ggttgatcct gcgggtcacc cagccgccct ggcgcttcga tcaccctcgc
     1561 cagatcttcg cagtcaacct gctgggagga ctcgccggca gcagcatcaa cgccaccctg
     1621 ggcaatatcg ttctgatggc gttcggcctg caggacatgc attccgttgg ttacgcctgg
     1681 ttcaactggt gggtgggcga caccatcggc gttttcttct tcacctccct gttgctgctg
     1741 atcgctaccc ccggccccta catcaccgga ctgcgcaaga actgggtggc catgcccatg
     1801 ctgctgatgt tcctgctggt catgctgctt ttcatcacca cccagcagta cgaacgcaac
     1861 cgcatactgg agcaactgcg caacgaatcg gacgacatca tgcacgagct gagcggccag
     1921 atagatacgg tgctcggcat cctggcgtcc atgcagcgct tccacagcgc gtcagagtcc
     1981 gtcacgcggg acgaattcac cgccttcacg gccggcatcc tcgccaccac gcccagcatt
     2041 gccgccctgg aatggattcc ggaagtttca ctggcgcaac gggaggcttt tgaggcacgg
     2101 ctgcaggcag aaggtttcgc ccaattccgt tttactgaaa tcagcgacaa cggcgagctg
     2161 gttacggcag gcgtgcgccc tttttattat cccgtcattt atgtggaacc gctgcagcgc
     2221 aatctcaaag cactgggact ggacctcggc tcacatcccg cccggctgga cgccatcggg
     2281 cgcgccctgg caagcaacca gttcgccgcc acccgcgcat tgcatcttgt acaggacagc
     2341 aacagttcat cacccgcagt gctgatcttt gaaccactac agcaccacgc gtccggcgaa
     2401 cgcagtctgc tgctggaaat cgtccgcttc agcgcgctgg tggataccgt gatgcccatc
     2461 gcccaccgca aggcaatttc ttttaccatc atgggccagg acatgcggca ggtgctcatt
     2521 aaccagagcg gcgaaaccgg gcatgctcaa ccactggtgg agcccaaggg cacccgcgtt
     2581 gtgcatgacc tgaaaattgc cgaccagacg tggaaagtga tattccccct cgacaagcgc
     2641 tacctggacc acaactccac ctggtatgtc tgg
//
LOCUS       DASUEG010000330         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1419339, whole genome shotgun sequence.
ACCESSION   DASUEG010000330 DASUEG010000000
VERSION     DASUEG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419339"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1898)
                     /locus_tag="VFX11_06165"
     CDS             complement(156..1898)
                     /locus_tag="VFX11_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938240.1"
                     /translation="MAPEQCLQVESGNVQLAVNIYGEANSPTLILVHGYPDCSDVWRG
                     LIPQLSQRFRVVTYDVRGTGQSTAPQSTQDYALEQLAADFRAVLDAVSPDQPVHLVAH
                     DWGAVQSWESVTDPQLQARIKSFSSVSGPCLDHIGFWIRDALQTLSPKDLAAVGKQVL
                     GSWYVWFFQLPGLAPLLWKNVLGKHWHRMLASRQGVVAPPSATQERDGVNGIELYRAN
                     IGRLANPQQRSTSIPVQLVIPVNDDFVSIPMARDSVRPWVENLWCREVSAGHWMPLSH
                     PQLLANMISEFVDFNEGRPETGVLKRARIARQDKPLGGKLAIVTGAGSGIGRETLLAL
                     ARAGAEVVAADINQAAAEESAQLAWDLGAVAHSYKVDVGNAKAMEKFAGWVQQQLGAP
                     DIVVNNAGIGVAGKFLDTHTADWEKVLHVNLWGVIHGSRLFAQQMVDAGIAGTIVNVS
                     SMAGYTPTRMMPAYATSKAAVLMLNNCIRAELAHDSIQVVSVCPGVIDTPITRSTFFV
                     GVDSAEQERLRDSAQRFYHRRNLPPRAVAEAIVKAIQSGREEIPVGIEAKLGRWLSYV
                     PGLARKMARMELDL"
     gene            complement(1964..>2175)
                     /locus_tag="VFX11_06170"
     CDS             complement(1964..>2175)
                     /locus_tag="VFX11_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938241.1"
                     /translation="GVMTKRVRDQAAFARWHYILDPTHICFFSDRTFGWIATHWGARL
                     EWLGDDVVLLQKNDVTEQYGAGIHR"
BASE COUNT          414 a          731 c          607 g          423 t
ORIGIN      
        1 tcaaggccct ttttttattc gcgaagtttt attcgcgaag cattattctg gaggttttgt
       61 tcgggaagct cttggttctg aaaactcggc tcagaaagat ttgttcagat tgtttgattc
      121 aatttgcatt gtccaaccgg cattattccg caccgctaca gatccagttc catcctcgcc
      181 attttgcgcg ccaggcccgg cacgtaactc agccagcgcc ccagcttcgc ctcgatgccc
      241 accggaatct cttcccgccc ggactggatg gctttcacaa tggcctccgc caccgcccgc
      301 ggcggcaggt tgcggcggtg atagaagcgc tgggcactgt cccgcaaccg ctcctgctcg
      361 gcactgtcca caccgacgaa aaaggtcgac cgggtgatcg gcgtatcgat cacgccgggg
      421 cagacactca ccacctgaat actgtcatgg gccagttccg cccggatgca attgttcagc
      481 atcagcaccg ccgcctttga ggtggcatag gccggcatca tgcgcgtggg cgtgtaaccc
      541 gccatcgacg acacgttgac aatggtgccc gcaatcccgg catccaccat ctgctgcgcg
      601 aacaggcgtg aaccgtggat cacaccccac agattcacgt gcagcacttt ctcccagtcc
      661 gcagtatggg tgtcgaggaa ttttcctgcc acgccgatgc ccgcgttgtt caccacaata
      721 tccggcgcgc ccagttgctg ctgcacccag ccggcgaatt tttccatggc cttggcgttg
      781 cccacatcca ccttgtagga atgggcaacc gcacccagat cccacgccag ttgcgccgat
      841 tcctccgccg ctgcctgatt gatgtcggcc gccaccacct ccgcaccggc acgggccagt
      901 gccagcaggg tttcacgacc gatgcccgag cccgcgcccg tcacgatcgc cagcttgccg
      961 cccagcggct tgtcctgccg ggcaatccgg gcccgtttga gcacacccgt ttccggccgg
     1021 ccttcgttga aatccacaaa ttcggaaatc atgtttgcca gcaattgcgg gtggctcagc
     1081 ggcatccagt gaccggccga cacttcgcgg caccagaggt tttccaccca gggccgcacg
     1141 ctgtcacgcg ccatgggaat ggagacgaaa tcgtcgttga cgggaatgac cagctgcacc
     1201 ggtatcgacg ttgaccgctg ctgcggattg gccagccggc cgatgttggc gcggtacagt
     1261 tcgatgccgt tcacgccgtc acgctcctgc gtggcactgg ggggcgccac cacaccctga
     1321 cggctcgcca gcatccggtg ccagtgtttt cccagcacat ttttccacag cagcggcgcc
     1381 agccccggca gctggaagaa ccacacatac caggaaccca gcacctgctt gcccacggcg
     1441 gcgaggtctt tcggcgacag ggtttgcagc gcgtcgcgta tccagaatcc gatgtgatcc
     1501 aggcagggcc cggatacgga tgaaaaggat tttatccgcg cctgcaactg cggatcagtc
     1561 accgactccc agctttgcac cgcgccccag tcatgggcca ccagatgcac gggttgatcc
     1621 ggactcaccg cgtccagcac ggcgcgaaaa tccgccgcca gctgttccag cgcgtaatcc
     1681 tgtgtcgact gcggcgccgt ggactgaccg gtgccacgca catcgtaggt caccacgcga
     1741 aaacgctggc tcagctgcgg aatcaaaccg cgccagacgt cgctgcaatc ggggtaaccg
     1801 tgcaccagga tcagggtggg cgagttcgct tcgccataaa tgttgaccgc cagctgcaca
     1861 ttgccggact ccacctgtaa acactgctcg ggcgccatga aaaaatgctc ctgaaatctg
     1921 tctgccgatg ccgcctggcc aatcgccgga cggggccccc agcttagcga tggatgccgg
     1981 cgccgtactg ttctgtcacg tcatttttct gcagcagcac gacgtcgtct cccagccatt
     2041 ccagccgcgc cccccagtgg gttgcgatcc agccaaacgt gcggtcactg aaaaagcaga
     2101 tgtgggtggg atcgagaata tagtgccagc gcgcgaaggc cgcctgatcg cgaacccgct
     2161 tggtcatgac gccga
//
LOCUS       DASUEG010000331         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_617043, whole genome shotgun sequence.
ACCESSION   DASUEG010000331 DASUEG010000000
VERSION     DASUEG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617043"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFX11_06175"
     CDS             <1..383
                     /locus_tag="VFX11_06175"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX4938242.1"
                     /translation="IAIAFIPLRYVVRYLGQPHHNRLLSVPVSPDQQQQARDIGRLTA
                     GMARITPWQSQCLVQVLAAKTLLARQHIPWVVHFGARHGQQGDLNAHAWLSVGPDIIV
                     GRRGHRTFGIVATYASVAATGKAF"
     gene            complement(391..2358)
                     /locus_tag="VFX11_06180"
     CDS             complement(391..2358)
                     /locus_tag="VFX11_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX4938243.1"
                     /translation="MSDIAGVFHWPGMQPVTSNLLNTLRQGLSAPGDASAGSWHDPLC
                     GMVQIARPLTPQDFFDHQPLRHPDAGVTLVAAARLDYREELCERLGIAAARCETLPDS
                     ELILAAYLNWGEDCVRYLAGDFAFAIWNSGTRRLFCARDPRGDMPFLFVHEAQRRFAF
                     ATALPALLALPGVDARLNLRMLADRLTFNGAHDYTQTFVEPIQLLPAAHCIRIDAQGV
                     GQRRYWDAPPAQSLQLRSSDDYVDCFRERFTRAVTSRLRCARPVGAHLSGGLDSSSVS
                     ALAARALAQRNEPLQTFTQVPIPSYRHQLRAGASPDDRHLVMKIAQQQTNIVPHLVDI
                     QGLTLEHELDSFFALSGMPPLNPFNRLWCERIQRQAARQQLGMMLTGSGGNFTVSCGV
                     MEPHLDQLARSGRWVRLWRDVRQAAAVQQVSPLGLLARQARELTPFSSLGLSKFQRVM
                     RQRQTGMIHPAFAGQMQVDQRLAALDRSKAGSTADFFEQVRRQRVLRAQNQSPLFWHT
                     MARQYGVYCCDPTNDVRVVEFCLAVPYEQQFRQGWDRALIRHAMDPYLPKEVIWRQQR
                     GKQSADWMLGFEQDLDLLQRDVDAMRHSPVAAEVLDLARMQALLQNWRGGAAVYSNSE
                     EVYHSLLLRAWLVGRFILWFEQRTAAVAAAA"
     gene            2450..2947
                     /locus_tag="VFX11_06185"
     CDS             2450..2947
                     /locus_tag="VFX11_06185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4938244.1"
                     /translation="MLNSLQLRPITATDTDTLFSIYASTRLDELQPTGWSDAEKQRFL
                     LQQFNAQHRYYQDIYTGARFDLVIAQGAVAGRLYVARWPAEIRIIDIALLPSFRRQGI
                     GATLLQQLLDEGDQAGQRISIHVEISNPARRLYEKLGFSKAEDLGLYWRMEREPRALP
                     CPDIA"
     gene            complement(2949..3272)
                     /locus_tag="VFX11_06190"
     CDS             complement(2949..3272)
                     /locus_tag="VFX11_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938245.1"
                     /translation="MEGPSHDDFTRSTGQLYHLAPHSADHRQLTAIRLAQVSPLKSCG
                     ATFESFSLLFDADKTAGLLGQGTYELANDGIGVHPIFITPVLHAAGQADKHYYEAVFT
                     RRKKA"
     gene            3442..3951
                     /locus_tag="VFX11_06195"
     CDS             3442..3951
                     /locus_tag="VFX11_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX4938246.1"
                     /translation="MSDPFLGEIRLLGFSFAPKGWALCNGQILNVNQNQALYSLLGNQ
                     FGGSAPTTFALPDLRGRVPVHISAGVPQGSVGGSESVTLTLNNMPAHTHTLMATSQAG
                     DRNGVAATRNLAQLTSGSAYAEPGGTATTLNSQAVSPTGGSTAHSNLQPYQVINFAIA
                     LSGLYPSRP"
     gene            3975..4493
                     /locus_tag="VFX11_06200"
     CDS             3975..4493
                     /locus_tag="VFX11_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX4938247.1"
                     /translation="MDPFVGEIRLFAGNYAPENWALCDGSTLQISTYNALYSLLGTTY
                     GGDGVSNFKLPDLRGRLPLHQGTGPGLANYPIGSSGGSETVTLSTAQLPAHSHGLVAV
                     SAAGGVGTPQNNLLAKATTTIYAAPQAGRTATLSSTTIANAGGNQPHDNLMPFCCINY
                     IIALQGIYPTQN"
     gene            4504..5037
                     /locus_tag="VFX11_06205"
     CDS             4504..5037
                     /locus_tag="VFX11_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX4938248.1"
                     /translation="MADQFVGEIRLLPFMFVPEGWASCNGAQLPVSQNQVLYAVIGNQ
                     FGGNANFFTLPNLQGSAPMGAGNGNGLTPRTLGQSGGAASVTLLPTQMPAHQHSCTGN
                     GFPGTSDTPDNSMLFGGEADPKFFLYKENPGNQDLVAMSPQALQNSGGNQPHANQQPW
                     LAVQFCIALDGAFPVRP"
     gene            5076..5264
                     /locus_tag="VFX11_06210"
     CDS             5076..5264
                     /locus_tag="VFX11_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938249.1"
                     /translation="MNRSTDNPSTESAQQTGMEETSRQGQPGRRKSWQQPILKKLDMR
                     ETLLNLSAVNPEGPGAYS"
     gene            complement(5281..>5789)
                     /locus_tag="VFX11_06215"
     CDS             complement(5281..>5789)
                     /locus_tag="VFX11_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938250.1"
                     /translation="IFTVASAGGRLDFRHTLLNMLWLGSDEPRMRQLKSAMIEQLPRS
                     IIGNRGAGITQQDLAGFSCGQTGYYSWLVQHMYGARLQQPDTLLGHFENLREDLLRLL
                     LASGCNLTAAMVQRLRTAAPANASSRGITAEYYDAETWQAVAQQEAWICRRFDYPARQ
                     SDSVAVPE"
BASE COUNT         1180 a         1794 c         1710 g         1105 t
ORIGIN      
        1 aaatcgcgat tgcttttatc ccgctgcgat atgtggtgcg gtatctcggc cagccgcatc
       61 acaatcgttt actgtcggtg ccggtatcac ctgaccagca gcaacaggcg cgggatatcg
      121 gccggcttac tgccggcatg gcgcgcatca cgccgtggca atcccagtgt ctggtgcagg
      181 tgctggcggc aaaaaccttg ctggcccggc agcacattcc ctgggtggtg catttcggcg
      241 cgcgtcacgg ccagcagggc gatttgaacg cgcacgcgtg gctgagcgtg gggccggata
      301 tcatcgtggg ccgacgcgga catcgcacgt ttggcattgt ggcgacgtat gcgtcggtgg
      361 cagcgaccgg aaaggcattc tgaatctggc tcaggccgct gcggccaccg ctgcagtgcg
      421 ctgttcgaac cagagaatga atcgccccac cagccaggcc cgcaacagca acgagtgata
      481 gacttcctca ctgttgctgt aaacggccgc gccgccgcgc cagttttgca gcaacgcctg
      541 catgcgcgcc aggtccagca cctcggcggc cacgggcgaa tgacgcatgg cgtccacatc
      601 ccgctgcaac agatcgagat cctgttcgaa tcccaacatc cagtcggcgg actgcttgcc
      661 ccgttgctgg cgccagatca cctctttcgg caggtaaggg tccatggcgt gacgaatcag
      721 tgcccggtcc cagccctggc ggaattgctg ctcgtaaggc acggccaggc agaattccac
      781 cacccgcaca tcattggtgg gatcgcagca atatacgcca tattgtcgcg ccatggtgtg
      841 ccagaacaac ggtgattgat tctgcgcgcg cagaacccgt tgtcgccgca cctgttcgaa
      901 aaagtctgcg gtggagccgg cctttgatcg atccagcgcc gccagccgtt gatccacttg
      961 catctgaccg gcaaatgcgg gatgaatcat cccggtctgg cgctggcgca tgacgcgctg
     1021 aaatttcgac agacccaggc tggaaaatgg cgtcaattcc cgcgcctgtc ttgccagcaa
     1081 tcccaacggg gacacctgct gcacggcagc agcctgccgc acatcgcgcc agagccggac
     1141 ccagcgtccg ctgcgcgcca actggtccag gtgcggctcc atcacaccgc aggacaccgt
     1201 gaaattgccg ccggaaccgg tcagcatcat gcccaactgc tggcgcgcgg cctgacgctg
     1261 gatgcgttcg caccacaggc gattgaacgg attgagcggc ggcatcccgg acaatgcgaa
     1321 aaacgagtcg agttcatgct ccagcgtcaa accctggata tccaccaggt ggggtacgat
     1381 attcgtctgc tgctgcgcaa tcttcatcac caggtggcgg tcatccggac tggcccctgc
     1441 gcgcagttga tgacggtagg atggaatcgg cacctgggtg aatgtctgca acggctcatt
     1501 gcgttgcgcc agcgcccgcg ccgccagcgc cgacacggaa gaggaatcca gtccgccgct
     1561 gaggtgcgca cccacgggcc gcgcacaccg caaccggctg gtgaccgcgc gcgtaaaccg
     1621 ttcacgaaag caatcgacat agtcgtcgct gctgcgcaat tgcagcgatt gggccggtgg
     1681 cgcatcccaa taccggcgtt gcccgacacc ttgcgcgtcg atccggatgc aatgggctgc
     1741 gggcaacagt tgaattggct cgacaaaggt ctgggtgtaa tcgtgggcgc cgttgaacgt
     1801 caggcggtcc gccagcatgc gcagattcag ccgcgcatcc acgccgggca atgccagcaa
     1861 cgccggcagc gctgtggcaa atgcaaaccg gcgttgcgct tcatgcacga aaaggaacgg
     1921 catatcgcct cgcggatcgc gcgcgcagaa cagccggcgc gtaccgctgt tccagattgc
     1981 aaacgcaaaa tcccctgcca ggtaacgcac gcagtcttcg ccccagttca ggtaagccgc
     2041 cagaatcagt tcactgtcgg gcagggtttc gcagcgggca gcggcaattc ccaggcgttc
     2101 acacaactcc tcgcggtagt ccagccgtgc cgctgctacc agtgtgacgc cggcatccgg
     2161 atgccgcagc ggctgatgat cgaaaaaatc ctgtggtgtg agcggccggg caatctgcac
     2221 cattccgcac agcggatcat gccagctgcc ggccgacgca tcacccggtg ccgacagacc
     2281 ctggcgcagg gtattcagca ggttcgaggt gaccggctgc atgcccggcc agtgaaaaac
     2341 gcccgcaatg tcactcatgt ccgcgctacg ctccgtgtca ttcttttctg gacaacctga
     2401 caacgctttc ctataccttg tgcatttgcg actgccagga aggcatccca tgctcaattc
     2461 actgcaactg cgccccatca ccgcaaccga caccgatact ctgttcagca tctacgccag
     2521 cacgcgactg gatgagctgc agcccaccgg ctggagcgat gctgaaaaac agcgctttct
     2581 gctgcagcag ttcaacgccc agcaccgcta ctatcaggac atctacaccg gtgcgcgctt
     2641 cgatctggtg atcgcacagg gcgccgtggc cggtcgtctg tatgtggcgc gctggcccgc
     2701 tgaaatccgc atcatcgata ttgcactgct gccttcattc cggcgccagg gcattggtgc
     2761 gacactgctg cagcaattgc tggatgaagg cgaccaggct ggccagcgca tcagcatcca
     2821 cgttgaaatc agcaatcctg cccgcaggct gtatgaaaaa ctgggatttt ccaaggccga
     2881 ggatctgggt ttgtactggc ggatggaacg ggaaccccgc gcactgccct gcccggacat
     2941 cgcctgaatc aggccttttt gcgccgggtg aataccgctt cgtaatagtg tttgtccgcc
     3001 tgtccggccg catgcaggac gggcgtgatg aagatgggat ggacaccgat tccgtcattg
     3061 gcgagttcgt aggtgccctg ccccagcaat ccggcggtct tgtccgcatc gaacagcagg
     3121 gaaaaggatt cgaacgtggc gccgcaggat ttgagcgggc tgacctgtgc cagacggatg
     3181 gcggtcagtt gacgatgatc agctgaatgg ggagcaagat ggtatagctg gccggtactg
     3241 cgggtgaaat catcatggct ggggccttcc atgggctgct ccttgtccct gtgcatattt
     3301 cattggcgcc aaattgacag cggtgattca gttttaggtg acagcccacg tcatgtcaat
     3361 tttctgttgg cggtttccgt caatggggtc tagggtttta agtcaggaca gtttcagctt
     3421 tacatcacag ggaggtgctc aatgagtgac ccgtttttag gtgaaatccg cctgctgggt
     3481 ttcagctttg ccccgaaggg ctgggcgttg tgcaacggcc agatcctgaa cgtcaaccag
     3541 aatcaggctt tgtattccct gctgggaaac cagtttggcg gctcggcgcc caccaccttt
     3601 gctttgccgg acctgcgcgg ccgggtaccc gtgcatatca gtgcaggggt gccccaggga
     3661 agcgtgggcg gcagcgaatc cgttacgctc acgctcaaca atatgccggc gcacacgcac
     3721 acgctgatgg ccaccagcca ggcgggtgat cgcaatggtg tggcggctac ccgcaatctt
     3781 gcccagctga ccagcggatc ggcctacgcc gagcccggtg gcacagcaac aacgctgaac
     3841 agccaggcgg tgtcgccaac cggtggttcc actgcacatt ccaacctgca gccgtaccag
     3901 gtgatcaatt ttgcaattgc attgtccggg ctgtatccgt cacgcccgta aacccatttc
     3961 gacaaggagt gttcatggat ccgttcgttg gcgaaatccg gctgtttgcc ggcaactatg
     4021 caccggaaaa ctgggcgctg tgcgatggca gcaccctgca gatttcaaca tacaatgcgt
     4081 tgtattcgct gctgggtacc acctatggcg gcgacggcgt cagtaacttc aagctgccgg
     4141 atttgcgcgg caggctgcct ttgcatcagg gcacaggtcc ggggcttgcc aattacccca
     4201 tcggcagcag cggcggcagt gaaaccgtca cgctcagcac ggcgcagctt cccgcacaca
     4261 gccacgggct ggtggcagtt tccgcagcag gaggtgttgg aaccccgcag aacaatctgc
     4321 tggccaaggc aaccaccacc atttacgcag cgccccaggc cggcaggacc gcgacgctgt
     4381 cctccaccac gattgcaaac gcgggtggca accagcctca cgacaatctg atgccgttct
     4441 gctgcatcaa ttacatcatc gccctgcagg gaatatatcc cacccaaaat tgaggaaacg
     4501 atcatggctg atcaattcgt tggcgagatc cgcttgttgc cgttcatgtt tgtgccagag
     4561 ggctgggcca gctgcaacgg tgcgcagttg ccggtgagcc agaatcaggt gttgtacgca
     4621 gtgattggca atcagtttgg tggcaatgcc aattttttca cgctgccgaa tctgcagggc
     4681 agtgcgccga tgggcgccgg taacggcaac gggctgacgc cgcgcacact gggccaaagc
     4741 ggcggcgctg ccagcgttac cctgctcccg acccagatgc cggcacatca gcacagctgc
     4801 acgggtaacg gctttcccgg caccagcgac acgccggaca acagcatgct gttcggtggt
     4861 gaagccgatc ccaaattctt tttgtacaag gaaaatccgg gcaatcagga tctcgtggcc
     4921 atgtcgccgc aggcattgca gaacagtggc ggcaatcagc ctcacgccaa ccagcagccc
     4981 tggctggccg tgcaattctg cattgcgctg gatggtgcat ttcccgtaag gccctgatcg
     5041 aatccgattc acaccactgc aaagggagac atgctatgaa tcgcagcacc gacaatccct
     5101 ccaccgaatc tgcacagcaa acgggtatgg aggaaacctc acgtcagggc cagcccggcc
     5161 ggcgcaaaag ctggcaacag ccgattctga aaaaactgga catgcgggaa accctgttga
     5221 acctgagcgc cgtcaatccg gaaggccccg gggcttattc ctgaggttga ttcccgagat
     5281 ctattccggc accgcgacgc tgtctgattg gcgagctgga taatcgaatc gccggcaaat
     5341 ccaggcttcc tgctgcgcca ccgcctgcca ggtttcggcg tcgtaatatt cggcagtgat
     5401 cccacggctt gaggcattgg caggcgcagc cgtgcgcaat cgctgaacca tagcagccgt
     5461 caggttgcag cccgacgcca gcaacagacg cagtaaatcc tcccgcagat tttcaaaatg
     5521 gcccagcagc gtatccggct gctgcaggcg cgcgccgtac atatgctgca ccagccagct
     5581 gtaataaccg gtctgcccgc agctgaaacc cgccagatcc tgctgcgtga taccggcgcc
     5641 ccggttgcca atgatggagc gcggcagctg ttcgatcatg gcggatttca gctggcgcat
     5701 gcgcggttca tcgctgccca gccacagcat attcagcagc gtgtggcgga aatccagacg
     5761 tccaccggcg gacgccaccg tgaaaatcg
//
LOCUS       DASUEG010000332         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_278306, whole genome shotgun sequence.
ACCESSION   DASUEG010000332 DASUEG010000000
VERSION     DASUEG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278306"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5359)
                     /locus_tag="VFX11_06220"
     CDS             complement(<1..5359)
                     /locus_tag="VFX11_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4938251.1"
                     /translation="MPLTQVDDDGLTRQDVELKRLAAIELMTPFNLAQGPLFRSRLFR
                     LNDHEHVFAVVIHHIVTDGWSMNLLVRDLANAYVQISVHGDVYFEPAPLQYADFAAWQ
                     RARLTDAVLQRMLGYWKDTLADVPALNLPTDFKRPPVQTYNGASVRFSVPAATRTQLA
                     ALAQTENASLFMSLLAGFAVLLQRYAGQNDFCIGTPVAGRDRSELENMVGFFVNTLAI
                     RTQLQPDASFRQLLQQVKTRVLDGFAHQDVPFEQIVEAVDPARDMSRSPLFQVMLAYQ
                     NLPVEQSNTGELAAAGDIQLEPYNPGIDAAKFDLTLTLWDEAGGLGGTIQYNTDLFAE
                     STLQAMMQHFTNLLACLAQQPDADIARHQYLSKDEVEQQLVEWNLTALEYDRSLSLPQ
                     WFDTSAAQHSTSVAIVCGNEQLTYAQLQQQTNQFAHYLQQQGVQPGDRVAVCLDRNLH
                     LMTAILGVIKAGATYVPLDASYPEERLRYILDNAQIKIGVTRAHLTANLPASINWIQW
                     DIAQAAIAAQPASAPAVAHDPERLLYVIFTSGSTGKPKGTSVYHRSEINLLHWYINQF
                     NMQSSDRFLLLSAVGFDLTQKNLFGPLLCGATLVIPDFQEYDINAIQQVIEQRQITWI
                     NCAPSAFYPLQDEMQQWQGLQSLRYLFLGGEPINLPRLANWLRQSRCQLVNSYGPTEC
                     TDIAAYYEIDVERDLSASALPIGRPNYNVRLYVLGEQQELLPVGAIGELYISGDGVGP
                     GYLNNAELTAQAFLPNPHLPGDIIYRTGDRVRYRTDGVIEYLGRRDHQMKLRGYRIEA
                     GEIQAVINNVPGVKDSLVDVITSTSNAQRLVAWVMTDAQAQNPVTTAQLSQQALAFLP
                     VFMVPESWVLLDTFPLTPNGKVDRKALPKPDIDSGRDYVAPRTALEISLSQLWGSVLG
                     VERVGVQDNFFQLGGQSLLATQLISRMSRQFSRTFSVRSLFENPTIEAFARIVEQSGT
                     EPSRPNLVARSQRDRARLSFGQQRLWFFEQINPGTSANNMPMGLKIRGALNEDALQKA
                     FRELIRRHESLRTHFASGADGEPVQLIDAAVQWNLQILDLTQLPASQREPQLQQQIAL
                     NQSAVFDLAQAPLLRATLVKSDTAEFHLLFCMHHIISDGASLVVLFRELVTLYLAFTR
                     NAPSPLPELRLHYGDFAEWQREWLSAERLNDQLQYWQNKLTKAPALLDLPLDQPRPPI
                     QSTAGASVNVQFPDAFGARLKMFCADNGITPFMFMLTGWKLLLSRYAGQTDICIGVPT
                     LGRQTPELESIIGFFIQSLVLRTDLGHNPTVAQAVQRVRQTVLEGFSHGDVPVDLIVE
                     RLGIRRNPAYSPLVQVAFQLLDNASFNAGNLLQSGQFGDLQLEMLGGNTTTAKFDLTL
                     SLTQNGDQFAGSLEYNTALFFDSTIQRLLQHYQLLCEQMLAAPQYPVDTLPLCAPQNL
                     LADLGLEPQQYEAAWPLSAMQYDMFMDNLVNPSSLQSSHGWHIHVHRELDVNLWRDCL
                     QTISDHQPILRSRFVTADKPWLDMGYLAIRRSHAIPFSVMDLSAASVDATELQLKIRA
                     LIYRPYDLARDELVSYSVLKLAPDHFVVITSVHHAILDGAALNSLWEQVTALYTRKQQ
                     GSTDGFRHAPAVFDQFVQHDRTSMDSDDVLAFWRDRFKQVEPLDFTVPAPVPAPAHFI
                     TRELFLDDDHWQQLKRFCREHRITPALYLKSLFGLMIRAYCRPDADFTIQETMGGRIK
                     GHHDTMGCYIQEIPFVFSRDVMKAEASIGDLLAYARQFQKEIKDQRLISIGKQIALSP
                     RG"
BASE COUNT         1099 a         1567 c         1652 g         1062 t
ORIGIN      
        1 ggcccctggg cgacagcgcg atctgcttgc caatggaaat caggcgctgg tcctttattt
       61 ccttctggaa ctgacgcgcg tacgccagca gatcgccaat gctggcctcc gctttcatga
      121 catcgcggct gaacacgaac ggaatttcct ggatgtagca gcccatggtg tcgtggtggc
      181 ccttgatacg gccgcccatg gtttcctgga tggtgaaatc cgcgtcggga cgacagtagg
      241 ccctgatcat caggccgaac agtgacttca aatacagcgc tggcgtgatg cgatgctcgc
      301 ggcagaaccg cttcagctgc tgccagtggt cgtcatccag aaacagctcg cgcgtgatga
      361 agtgcgcggg cgcagggacc ggtgcgggca cagtgaagtc cagcggctcc acctgtttga
      421 accggtcgcg ccagaaagcc aatacatcgt cgctgtccat gctcgtgcga tcgtgctgca
      481 cgaattgatc gaagacggcc ggtgcatggc ggaagccatc cgtgctgccc tgctgcttcc
      541 gtgtgtagag cgctgtcacc tgctcccaca agctgttgag cgccgcgccg tccagaatgg
      601 cgtgatgaac cgaggtgatc acaacgaaat ggtcaggcgc gagtttcagc acgctgtagc
      661 tgaccagttc atcccgcgcc agatcgtaag gccggtagat cagggcacgg attttcaact
      721 gcagttctgt tgcatcgacc gacgctgcgc tcaggtccat aacgctgaac ggaatggcat
      781 ggctgcgccg gatggccaga taacccatgt cgagccaggg tttgtcggcg gtgacaaagc
      841 gggaacgcag aatcggttga tgatcgctga tggtttgcag gcagtcgcgc caaaggttca
      901 cgtccaattc acggtgaacg tggatgtgcc agccgtggct gctctgcagc gacgatggat
      961 tcaccaggtt gtccatgaac atgtcgtact gcatggccga caacggccag gcagcttcgt
     1021 actgttgcgg ttccaacccg agatccgcca gcaaattctg cggtgcacac agcgggagtg
     1081 tgtcgactgg atactgcggt gccgccagca tctgctcgca cagcaactgg taatgctgca
     1141 gcaggcgctg gatggtgctg tcgaaaaaca gcgccgtatt gtattccagc gagccagcaa
     1201 actggtcgcc gttctgggtg aggctgaggg tcagatcgaa cttggcggtg gtggtattgc
     1261 cgcccagcat ttccagttgc agatcgccga actgcccaga ctgcagcaga ttgccggcgt
     1321 tgaaggaggc gttgtccagc agctggaacg cgacctgcac cagcggtgaa tacgccggat
     1381 tgcggcggat gccgagacgc tcgacgatca ggtccaccgg cacatcgccg tgactgaaac
     1441 cttccagcac ggtctggcgc acccgctgca ccgcctgcgc aacggtcggg ttatgaccga
     1501 gatcggtacg cagcaccaga ctctggatga agaagccgat gatgctttcc agttccggcg
     1561 tctggcggcc cagcgtcggc acaccgatgc agatatccgt ctggcctgca tagcgtgaca
     1621 gcagcagttt ccagccggtg agcatgaaca tgaacggtgt gatgccgttg tcggcgcaga
     1681 acattttcag gcgcgcgccg aacgcatccg ggaactggac attgacgctg gcgccggccg
     1741 tggactggat gggcggacgc ggctggtcga gcggcaggtc cagcaacgcc ggcgctttgg
     1801 tcagcttgtt ctgccagtac tgcagctggt cattcaggcg ttccgccgac agccattccc
     1861 gctgccactc ggcgaaatcg ccgtagtgca atcgcaattc cggcagtggc gatggcgcat
     1921 tccgggtaaa cgccagatac aaggtgacca gttcgcggaa cagcacgacc agtgacgcac
     1981 cgtcggaaat aatgtggtgc atgcagaaca gcagatgaaa ttccgcagtg tccgatttca
     2041 ccagggtggc gcgcagcaac ggtgcctgcg ccagatcaaa taccgctgac tgattgagtg
     2101 cgatctgttg ctgcaactgc ggttcgcgtt gagatgcggg cagctgcgtc agatcgagta
     2161 tctgcagatt ccactgcacg gctgcgtcga tcagctgcac cggctcgcca tcggcgccac
     2221 tggcaaaatg cgtgcgcagg gattcgtggc ggcggatcag ttcgcggaag gccttttgca
     2281 acgcatcttc attcagtgca ccgcggattt tcaggcccat cggcatgttg ttggcactgg
     2341 tgccgggatt gatctgctcg aagaaccaca gccgttgctg gccgaacgac aatcgtgcac
     2401 ggtcacgctg gctgcgcgct accaagttcg ggcgcgaagg ctcggtaccg gattgctcca
     2461 cgatccgcgc aaaggcttcg atggtgggat tttcaaacag cgaacggacg ctgaacgtgc
     2521 ggctgaactg acgcgacatg cgactgatca gctgcgtggc cagcaacgac tgcccgccca
     2581 gctggaagaa gttgtcctgc acgcccaccc gttcgacgcc cagcacggag ccccacaact
     2641 ggctgaggct gatttccagt gcggtacgcg gcgccacata atcgcggccg ctgtcgatat
     2701 ccggtttcgg tagcgctttg cggtccacct tgccgttcgg cgtcagcggg aatgtgtcga
     2761 gcagcaccca gctttccggc accatgaaga cgggcaggaa cgccagtgcc tgctgcgaca
     2821 gctgcgcggt ggtaacggga ttttgcgcct gcgcatcggt catgacccaa gccaccagac
     2881 gctgcgcatt gctggtggac gtgatcacgt ccaccagcga atccttcacg ccgggcacgt
     2941 tgttgatcac tgcctggatt tcaccagcct cgatccggta gccacgcagt ttcatctgat
     3001 gatcgcgacg accgagatat tcgatcacac catcggtgcg ataacgcacg cggtcaccgg
     3061 tgcggtaaat aatatcgcct ggaagatgcg ggttcggcag aaacgcctgc gccgtgagtt
     3121 cggcattgtt caggtaacca gggccaacac catcgccact gatatacagt tcgccaattg
     3181 cgcccaccgg caacagctcc tgctgttcac ccagcacata caggcgcacg ttgtagttgg
     3241 gccggccaat gggcagcgcg gacgccgaca ggtcgcgttc cacatcgatc tcgtaatagg
     3301 ccgcgatgtc agtgcactcg gtcgggccgt aactgttcac cagctggcac cggctttgcc
     3361 gcagccagtt ggccagacgc ggcaggttga tcggttcgcc gccgaggaac aggtagcgca
     3421 acgattgcag gccctgccac tgctgcattt catcctgcaa cggatagaag gcgctgggcg
     3481 cgcagttgat ccaggtgatc tgccgttgct caatcacctg ctgaatggcg ttgatgtcgt
     3541 attcctggaa atccggaatc accagcgtgg cgccgcacag cagcggaccg aacagatttt
     3601 tctgggtgag atcgaaacca acggcgctca gcagcaggaa gcgatcgctg gactgcatat
     3661 tgaactggtt gatataccag tgcagcaggt tgatttcgga gcggtgataa acgctggtgc
     3721 ctttcggctt gccagtggaa ccggatgtga aaatcacata cagcaggcgt tcgggatcgt
     3781 gcgcgacggc aggcgcagaa gccggttgtg cggcgatcgc tgcctgtgca atatcccact
     3841 gaatccagtt gatcgacgcc ggcagattcg cggtcagatg cgcgcgggta acgccgattt
     3901 tgatctgcgc attgtccaga atatagcgca gacgttcttc cggataactg gcatcgagcg
     3961 gcacataggt ggcgccggcc ttgatcacgc cgagaatggc ggtcatcaga tgcaggttgc
     4021 gatccagaca tacggcaacg cggtcgcccg gctgcacacc ctgttgttgc agatagtgcg
     4081 caaactggtt ggtctgctgc tgcaactggg cgtaagtgag ctgttcattg ccacacacaa
     4141 tagcgacgga agtcgaatgc tgtgcggcac tggtatcgaa ccactgcggc aacgacagcg
     4201 agcgatcgta ttccagcgcc gtcaggttcc attccaccag ctgctgttcc acttcgtctt
     4261 tcgacagata ctgatggcgc gcaatgtcag catcgggctg ctgcgccaga caggccagca
     4321 gattggtaaa atgctgcatc atcgcttgca gcgtggattc tgcgaacagg tcggtgttgt
     4381 attggatggt accgccaagg ccgccggcct cgtcccacaa ggtcagcgtg agatcaaatt
     4441 tggccgcgtc gataccggga ttatagggtt caagctggat atcgcccgct gctgccagtt
     4501 cgccggtatt gctctgctcc accggcagat tctgataagc cagcatcacc tggaacaacg
     4561 gtgaacgact catgtcgcgg gcgggatcga ccgcttccac gatctgctcg aacggaacgt
     4621 cctgatgggc gaaaccgtcc agcacgcggg ttttcacctg ctgcagcaac tggcggaacg
     4681 atgcatccgg ttgcagctgg gtgcgaatgg ccagtgtgtt gacgaaaaag cccaccatgt
     4741 tttccagctc ggaacgatca cgccccgcta ccggtgtgcc gatgcagaaa tcgttctgtc
     4801 cggcatagcg ctgcagcagc acggcgaaac cggccagcag gctcatgaac agggacgcat
     4861 tttctgtttg cgccagtgct gccagctggg tgcgggtggc tgccggcacg ctgaaacgca
     4921 ccgaggcgcc gttataggtc tgcaccggcg gacgtttgaa atcggtcggc aggttcagcg
     4981 ccggcacatc cgccagggtg tccttccagt aacccagcat gcgctgcaat acggcatccg
     5041 tcagacgcgc gcgttgccac gcagcaaaat ccgcatactg caacggcgcc ggttcgaaat
     5101 acacatcgcc gtgcacggaa atctgcacgt aggcattcgc cagatcacgc accaggagat
     5161 tcatcgacca gccgtcggtg acgatgtggt gaatgactac agcaaatacg tgctcatgat
     5221 cgttgaggcg gaacaggcgc gagcggaaca gcgggccctg tgccagattg aacggcgtca
     5281 tcagctcgat ggcggcgagg cgtttcaatt ccacgtcctg gcgggtcaag ccatcatcgt
     5341 ccacctgtgt cagcggcaat ttccagtcct gcggcggcag
//
LOCUS       DASUEG010000333         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_376836, whole genome shotgun sequence.
ACCESSION   DASUEG010000333 DASUEG010000000
VERSION     DASUEG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376836"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFX11_06225"
     CDS             complement(<1..528)
                     /locus_tag="VFX11_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4938252.1"
                     /translation="MVERIIEWSARHQWMVLGLTLLLTLWALDSMRKTPLDALPDLSD
                     PQVIIFTDWMGRSPDLVEDQITYPLVRALQSTPGVSAVRGYSMFGMSFIYAIFDDGTD
                     IYWARTRVLEQLGRVRQLLPPGVAPILGPDASGVGWVFQYVLEDTTHQLDPAQLRALQ
                     DFTVRPALQAVTGVAE"
     gene            complement(531..1838)
                     /locus_tag="VFX11_06230"
     CDS             complement(531..1838)
                     /locus_tag="VFX11_06230"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4938253.1"
                     /translation="MTSRPKALSQWRPIIVGVVAGVLLLLLVVGIQHFRHGWPFSLHH
                     DFSAPDAASKVPVAVDHSAHAGSDAAQARVAVDLSEQQLQSIGVRLERVQRETITQPL
                     RAIATVVPDESRVSHVHARVSGWLEQLYVNTTGQAVRAGEPVAGVYSQDLFATQTEYL
                     SALKFGRAGPGGAVVESAVVEGARTRLKLLGMAEAEIADIERRGEARRLVTLTAPRSG
                     VVMHRGISAGTAVDPSTEILTVADLSTVWVWAEVPESGARQVAIGTTAQLDFPALGQE
                     PVEASVDFVYPTLTERTRTLRVRFSVPNQDGALRPGLFGTAIFQSSPHATLMISRDAV
                     VDTGMNQHVFVVVEPGRFEPRKVTLGARMPDRVEVLQGLAENEEIVASGVFLLDSESR
                     LRASGGGGTGHSGHGGSAPAAEPGTSEPASPDSKADVHSGHGE"
     gene            complement(1835..3178)
                     /locus_tag="VFX11_06235"
     CDS             complement(1835..3178)
                     /locus_tag="VFX11_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4938254.1"
                     /translation="MKATGFVFLPLLALLFPGFCLAQPEMPARWSLEAVVGMAREQRQ
                     DIIAARARADAARERPAIVSALEDPMLAPSIDHYPFDRMDSEEPMPASTEPPMEGEPA
                     PAPIASGSDDSDRRYDWSVSIEQRFPLSKVLTHRRHAAEADAEKAAAEAERTALDVEL
                     DAITAFYMVQEKRRMATVVQQQIVLARQFVNAANARYGSGSGNQSEVLRVEVEVARLE
                     SSARALQAGIRSAEAMLNTSIGRDTGMPVPDLVSPKLDNPLPSRDEVSAAALRQRPEL
                     QMGNAELSRAMADVQAMRSMYSPMGMVRVGYASTMAEGKGAMLMVGVSLPIWRDKLRG
                     GVAEARAMERMASADLESMKRMVEGDAIAARDQLDVAQIQYLALRDDVVPRAQRLIQP
                     TLSVYASGQGTLFAVIEAAQALWDVEAEFVMAETALGVAWARLDRMTGTTKDHTP"
     gene            complement(3459..>3707)
                     /locus_tag="VFX11_06240"
     CDS             complement(3459..>3707)
                     /locus_tag="VFX11_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4938255.1"
                     /translation="SDMRVWAIKSVALAAENLMLAFRAYGFDSCPMEGMDEKRVRKLL
                     NLPRDAEVVMVVGAGERAKDGVYFPRVKFDRKNFVRYV"
BASE COUNT          799 a         1261 c          978 g          669 t
ORIGIN      
        1 ttccgccaca cccgtaacag cctgcaacgc aggccgcacc gtaaaatcct gcaacgcgcg
       61 cagttgtgcc gggtccagct gatgcgttgt gtcttccagt acatactgga atacccagcc
      121 cacaccactg gcatcagggc cgaggatagg cgccacaccc ggcggcagca attgccgtac
      181 ccgccccaac tgttcgagca cgcgggtacg cgcccagtaa atatcggtgc catcatcgaa
      241 gatggcgtag ataaaactca tgccgaacat cgaatagccg cgcacggcac tgacacccgg
      301 cgtactttgc agcgcccgca ccagcggata ggtgatctgg tcctccacca gatccggact
      361 gcgccccatc cagtcagtaa agatgatgac ctggggatcg gataaatccg gcaaggcatc
      421 cagtggcgtc ttgcgcatgg aatccagcgc ccacaacgtg agcagcagag tcagcccaag
      481 caccatccat tgatgccgtg cagaccattc aataatgcgc tcgaccatgg ctactcccca
      541 tgaccgctat gaacatcagc cttgctgtcg ggtgacgctg gctcggaagt gcccggttcc
      601 gcagccggtg ctgatccgcc gtgacctgaa tgtcctgtgc cgcctccacc tgatgcacgc
      661 aaccggcttt cggaatccag cagaaacacg ccggaagcga cgatctcttc attctcggcc
      721 agtccctgca gcacttccac ccgatccggc atgcgcgcgc ccagagtgac cttgcgcggt
      781 tcgaaacgcc caggttccac cacgacaaac acatgctggt tcatgcctgt gtccaccacg
      841 gcgtcgcggg aaatcatcag cgtagcgtgc ggtgaactct ggaatatggc ggtaccaaac
      901 agaccgggcc gcaaagcgcc atcctgattc ggaacgctga aacgtacccg cagcgttcgc
      961 gtgcgttcgg tcagggtggg ataaacgaaa tccacgctcg cttcaaccgg ctcttgcccc
     1021 agcgcgggaa aatccagctg tgccgtggtg ccgatcgcaa cctggcgcgc acccgactcg
     1081 ggcacttcag cccacaccca caccgtggaa agatccgcca ccgtcagaat ttccgtggac
     1141 gggtccaccg ccgtgcccgc cgaaatgccg cgatgcatga caaccccgct gcgcggcgcg
     1201 gtcaacgtca ccagccgtcg cgcttccccg cgtcgttcaa tgtctgcaat ttctgcttcc
     1261 gccatgccga gcagtttcag ccgtgttctg gcaccttcca ccactgcgct ttctacaact
     1321 gcaccgcccg gccctgcccg accgaatttc agggccgaca gatattcagt ctgcgtggcg
     1381 aacagatcct gcgaatagac tcccgccacg ggctcaccgg cgcgcaccgc ctgcccggtg
     1441 gtattcacat atagctgctc cagccagccg gacacacggg cgtgcacgtg cgacacgcgg
     1501 gattcatccg gcaccaccgt agcgatggcg cgcaatggct gggtgatggt ttcccgctgc
     1561 acccgctcca gacgaacacc gatggattgc agctgctgct cgctcagatc caccgctacc
     1621 cgcgcctggg cggcatcgct gccagcatgc gcggaatgat ccaccgcaac gggaaccttc
     1681 gacgccgcat cgggcgcaga aaaatcgtgg tgaagtgaaa atggccagcc atggcggaaa
     1741 tgctgaatgc ccacaaccag cagcaacagc agaacgccgg caacaacgcc aacaataatg
     1801 ggccgccact gcgacagggc tttcggtcgg gatgtcatgg agtgtgatcc tttgtcgtac
     1861 cggtcatgcg atcaagacgg gcccaggcca cgcccagcgc ggtttctgcc atcacaaact
     1921 cggcctccac atcccacaac gcctgcgcgg cctcaatcac ggcaaacagg gtgccctggc
     1981 ccgaggcata aaccgacagg gttggctgaa tcaaacgctg cgcgcggggc acaacatcat
     2041 cccgcaaggc gagatactgg atttgcgcca cgtcgagctg atcgcgcgcc gcaatggcat
     2101 cgccctccac catccgcttc atggattcca gatcggcact cgccatgcgc tccatcgcac
     2161 gggcctctgc cacgccaccg cgcagcttgt cgcgccagat cggcaggctg acgcctacca
     2221 tcagcatggc acccttcccc tccgccattg tcgatgcata acccacacgc accatgccca
     2281 tcggggaata catcgagcgc atggcttgca catccgccat ggcacgggaa agttcggcgt
     2341 tacccatctg caattccggt cgctgccgca atgccgcagc agacacctcg tcacgggacg
     2401 gtaacggatt gtcgagtttc ggactgacca gatcaggaac cggcatcccg gtatcacgac
     2461 caatactggt gttgagcatg gcttctgccg agcggatacc cgcctgcaat gcacgcgccg
     2521 aggattcgag ccgcgccact tcaacttcaa cccgcaagac ttccgactga ttacccgaac
     2581 cggaaccata acgggcattg gccgcattca caaactgccg ggcaagtaca atctgctgct
     2641 gaaccaccgt cgccatccgc cgtttttcct gcaccatgta aaaggcggta atggcatcca
     2701 gttccacatc cagcgccgtg cgctcggctt cggctgcggc cttttcggca tcggcttccg
     2761 ccgcatggcg acgatgcgtc aacacttttg acaggggaaa acgctgctcg atactgacac
     2821 tccagtcata gcgccgatcg ctgtcatcgc tgccgctcgc aatcggggct ggcgccggct
     2881 cgccctccat ggggggctct gtcgacgccg gcatcggctc ttcactgtcc atccggtcga
     2941 aggggtagtg atcaatggac ggtgccagca tggggtcttc cagcgccgac acaatggcgg
     3001 gacgttccct ggccgcatca gcacgggccc gcgccgcaat gatatcctgg cgctgctcgc
     3061 gcgccatccc caccaccgct tccagcgacc agcgcgccgg catttccggc tgcgccagac
     3121 aaaatccggg gaacagcaac gcaagcaagg gaagaaaaac aaaacctgtc gctttcatag
     3181 gcataatgcc catttcgcct cgccaatcgg cattcgtcat tgaccgggac gcggtgcaaa
     3241 ccaccactgc ttctcgtgca gattcaatgt acgaaactat cacctgaaaa tcacattaca
     3301 aatctgtaat tcaggttaaa gtcggcaaaa gctgatttat gtcagaattt acgcccacct
     3361 cctgatgagc gggaattgct taccggcgac agtgcagctg cgacgggctg cactgctcat
     3421 atcgatacaa agttgccggg gcaccccctt atgcaaactc aaacataccg cacaaaattc
     3481 ttgcgatcga atttcacccg ggggaaataa acaccgtctt tggcgcgttc gcctgcgccc
     3541 acgaccatca ccacttcggc atccctcggc agattcagca gttttctgac gcgcttctcg
     3601 tccatgcctt ccatcgggca cgaatcaaac ccgtaagcac ggaacgccag catcaggttt
     3661 tcggctgcca gtgcaacact cttgatcgcc cagacccgca tgtccga
//
LOCUS       DASUEG010000334         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1878737, whole genome shotgun sequence.
ACCESSION   DASUEG010000334 DASUEG010000000
VERSION     DASUEG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878737"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFX11_06245"
     CDS             complement(<1..580)
                     /locus_tag="VFX11_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX4938256.1"
                     /translation="MSEQPQNTELHIDENKLIAERKQKLAELRDVGQAFPNDFRRDAY
                     CTDLQDRFGGKENEALEADKQYFSVAGRIMLKRIMGKASFTTIQDMTGRIQLYISKEA
                     VGEDTYTSFKKWDIGDIVGARGYVFKTKTGELSVHVEELRLLTKSLRPLPDKFHGMTD
                     QEMKYRQRYVDLIMSEDTRRAFRIRSQVIEGIR"
     gene            complement(668..1763)
                     /gene="prfB"
                     /locus_tag="VFX11_06250"
     CDS             complement(join(668..1690,1692..1763))
                     /gene="prfB"
                     /locus_tag="VFX11_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013660178.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX4938257.1"
                     /translation="MEINAHLNRIKDLSERAASLRGYLDYDLKKERLEEVMRELEDPT
                     LWNDPEKAQAIGKERVDLEGVVLTLDRLDSGLVDAKDLLDLAVEEDDEGTLADVSTEL
                     DSLEKSVADLEFRRMFSGEMDPSNCFIDIQAGAGGTEAQDWASMLFRMYLRWIDRHGF
                     KGEILEVSDGEVAGIKSATIRVEGPYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFTA
                     VFVSPEVDDDIEIEINKSDVREDTYRSSGAGGQHVNKTDSAVRLTHIPTNTVVAVQNE
                     RSQHANRDRAWKLLRAKLYELEMLKRNAEKQALEETKSDVGWGSQIRSYVLDQSRVKD
                     LRSNIESSNPNAVLDGDLDEFIEASLKMGL"
     gene            complement(1917..2063)
                     /locus_tag="VFX11_06255"
     CDS             complement(1917..2063)
                     /locus_tag="VFX11_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938258.1"
                     /translation="MFTLQQAARTVSVSLLAMAMAYGTVIVTFFNEPLRQGVNAPASV
                     EPWS"
     gene            complement(2260..>2531)
                     /locus_tag="VFX11_06260"
     CDS             complement(2260..>2531)
                     /locus_tag="VFX11_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007624246.1"
                     /note="5'-3' single-stranded-DNA-specific exonuclease;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX4938259.1"
                     /translation="WGQAFPEPLFHGDFHCLEQRVLKEKHLKMSVAHPASNRIFDAIA
                     FNVDRAQWPNEARKVRLAYRLDINEFRGTRSLQLRVEYMEPLDAK"
BASE COUNT          541 a          749 c          704 g          537 t
ORIGIN      
        1 ggcggatacc ctcaatcacc tgcgaacgaa tacggaaagc gcgacgcgtg tcttcgctca
       61 tgatgagatc gacatagcgc tggcgatatt tcatttcctg gtcggtcatg ccatggaact
      121 tgtccggcag cgggcgcagg gacttcgtca gaaggcgcag ctcttcaaca tgcaccgaca
      181 gctcgccagt cttggtcttg aagacatatc cacgggcacc gacgatgtcg ccaatatccc
      241 acttcttgaa gctggtgtag gtgtcctcac ccacggcttc tttcgaaatg taaagctgga
      301 tgcggcccgt catgtcctgg atggtggtga agctggcctt gcccatgatg cgcttgagca
      361 tgatgcgacc ggccaccgag aagtactgct tgtcggcttc caacgcctcg ttttccttgc
      421 cgccaaaacg gtcctgcagg tccgtgcagt aagcatcacg acggaaatca ttcgggaagg
      481 cctgaccgac gtcacgcagc tcggcgagtt tctgtttgcg ctcggcaatc agcttgttct
      541 cgtcaatgtg cagttcggtg ttttgcggct gttcactcat gtcttctacc ttttcgctct
      601 ccccttcgcg ctgaaggtgg agaaaaaatc acacatccgt ccacagtgac ggttacaggc
      661 ctatcgtcta caaacccatc ttcagcgagg cttcaatgaa ctcgtcgagg tcgccatcca
      721 gcacagcatt gggattggag ctttcgatgt tgctgcgcag atccttcaca cgggactggt
      781 ccagcacata cgaacggatc tggctgcccc agcccacatc ggacttggtc tcttccagcg
      841 cctgtttttc agcattgcgc ttgagcatct ccaactcata caacttggca cgcagcagct
      901 tccaggcgcg gtcacggttg gcatgctggg aacgttcgtt ctgaaccgct accaccgtat
      961 tggtcgggat atgggtcaga cgcacggccg agtcggtttt gttcacatgc tggccaccgg
     1021 caccggagga gcggtacgta tcttcgcgca catccgactt gttgatctcg atctcgatat
     1081 cgtcatccac ttccggcgat acgaagacgg cggtgaagga cgtatgacga cgattgccgg
     1141 aatcgaacgg cgacttgcgc accaaacggt gtacgccggt ttccgtacgc agccatccga
     1201 aggcatatgg gccctccacg cggatggttg ccgacttgat gccggcgact tcaccatcgg
     1261 acacttccag gatctcgccc ttgaagccgt gacggtcgat ccagcgcagg tacatacgga
     1321 acagcatgct ggcccagtct tgagcctcag tgccaccggc gcccgcctgg atgtcgataa
     1381 agcagttgct gggatccatc tcgccagaaa acatgcggcg gaactcgaga tcggcaacgg
     1441 acttttccag gctgtcgagt tcggtactga catcggccag cgtgccctcg tcatcctcct
     1501 ccacggccag atccagcaga tccttcgcat ccaccagacc actgtcgagg cgatccagcg
     1561 tcagcacaac gccctcgagg tccacacgct ccttgccgat ggcctgggct ttttccggat
     1621 cattccaaag cgtggggtct tcaagctcgc gcatcacctc ttcgaggcgt tcttttttca
     1681 gatcatagtc aaaggtaccc cctgagcgat gcagcacgct cggagaggtc cttgatgcgg
     1741 ttcagatggg cgttgatttc catgaaaaaa acagtggcga atggacaagg gcgcggattg
     1801 taccggatgt gggtacagcc ggccagaaag acccgccaca aacggaaccg gccgcaaagg
     1861 cggccggtcg ggacagggct cagcgtgcct gcggacgctt cacatcagga gcggcgctag
     1921 gaccagggtt cgacactggc cggggcattc accccctggc gcaacggctc attgaagaag
     1981 gtgacgataa cagtgccgta ggccatggcc atggcgagca ggctgacgga cacagtccgg
     2041 gcggcctgtt gcagggtaaa catgatgatc tccagtcgtt cagtcgtacc gcccgggaca
     2101 ggcccaatgg tgaaatcagg acccggacgg ctagccggtt cgcaagagcc tgacggctcc
     2161 agaccggtga tcaaatatta accgaaaacc atcttgaatg gcagaccgga tggttaccga
     2221 ctgtaagggc atccataatt atctagtaaa aacaaaatgt tatttagcat ccagcggttc
     2281 catgtattcc acccgcaact gcagggagcg agtgcctcgg aactcgttga tgtccagccg
     2341 atacgccaga cgcaccttgc gggcctcatt aggccactgc gctctatcca cattgaaggc
     2401 gatcgcatcg aaaatccggt tggatgctgg atgcgccacg ctcatcttga ggtgcttctc
     2461 cttcagcacc cgctgctcca ggcagtggaa atcgccatga aacaggggtt caggaaaggc
     2521 ctgcccccaa g
//
LOCUS       DASUEG010000335         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_676505, whole genome shotgun sequence.
ACCESSION   DASUEG010000335 DASUEG010000000
VERSION     DASUEG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676505"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..165)
                     /locus_tag="VFX11_06265"
     CDS             complement(40..165)
                     /locus_tag="VFX11_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938260.1"
                     /translation="MEQSPRKPRIPRELKRFIAAMMGIILFIAAASVVVALVVDQ"
     gene            304..759
                     /locus_tag="VFX11_06270"
     CDS             304..759
                     /locus_tag="VFX11_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938261.1"
                     /translation="MRKVSLFSVQSKQNTGRDPNCQLLLQQHLTGRTLRGRKLVDQFA
                     SGQRYVLITDDDNPFEEVLHIYLLDLELNVLDELDLCQPYTPGVYQLHRHDDRHISFR
                     FFDDRLWSLQIREEPRPSPLNYSRFPTRRPLKLWGRQYLELNRSESSAA"
     gene            complement(766..1281)
                     /locus_tag="VFX11_06275"
     CDS             complement(766..1281)
                     /locus_tag="VFX11_06275"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX4938262.1"
                     /translation="MDDRMEVWHGDITLLKVDAIVNAANNRLAGGGGVDGAIHRAAGA
                     ERLQEACRRIGYCETGQAVVTPGFALPARTIIHTVGPVWRGGTEGEAEQLRSCYSACM
                     TLAVREGARSIAFPAISCGVYGYPREDAVAIAVDEVERCLEVFLMIDRVVFACFDAGM
                     AELYRAVLGER"
     gene            complement(1317..1595)
                     /locus_tag="VFX11_06280"
     CDS             complement(1317..1595)
                     /locus_tag="VFX11_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938263.1"
                     /translation="METQCAQEIRELARQYCDGEFNKGEYRRRRRDILQRCVSEDFAP
                     HPDSLAEEEPVVLPQVHKGSPALSWLGVAMAIAALLVLGIMGFLLYSL"
     gene            complement(1610..2332)
                     /locus_tag="VFX11_06285"
     CDS             complement(1610..2332)
                     /locus_tag="VFX11_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX4938264.1"
                     /translation="MVQTHWLSSAATHCGAVRNMNEDAFLNRNNDGLWVVADGMGGHD
                     AGEVASEMITATLARLDVEQPLPDVVDALEDSLLDVHQKIRTYSRTHCEGRTMGSTVV
                     SLLVRESVGVCLWAGDSRLYRYRDHKLQKVSDDHSQVNEMLARGMITPEEAKNHPASN
                     VITRAVGASETLYVDVTLVELRKGDVYLLCSDGLYGALSEGKLVHHLRNPDVESCTQA
                     LIEDALKAGARDNVTVVTVHIQ"
     gene            complement(2464..>2695)
                     /locus_tag="VFX11_06290"
     CDS             complement(2464..>2695)
                     /locus_tag="VFX11_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938265.1"
                     /translation="PAAQGEWFGELERVALAGRRDGVTIVDLERWLNSIRMAGQDAMD
                     AAFRLLHRLLLLCFPSYSLWWSTGSEWICQRS"
BASE COUNT          558 a          857 c          726 g          554 t
ORIGIN      
        1 ctggaccggc tggaatggca gcccctgctg taaaacgcgt tattgatcca ctaccaatgc
       61 caccaccacg gacgccgcgg caataaacag gataatgccc atcatggcgg cgatgaagcg
      121 cttgagttcc cttgggattc ggggtttgcg ggggctctgt tccaacgtgg gttccacctt
      181 cggttatgtg aatttttcac agatcttcca ttcaatctgt cttacttggt tcaaatatta
      241 accagtagac tgacttcaaa tcacatgact ttccagccat ttgtcattat tcccatgatt
      301 cacatgcgaa aagtatcgct tttttctgtg cagtccaaac agaacactgg ccgcgacccc
      361 aactgccagc tgctgctgca acagcatctc accggtcgca ccctgcgcgg tcgcaagctg
      421 gtagaccagt tcgcatccgg acagcgctac gtgctcatta cggatgatga caacccgttc
      481 gaggaagtgt tgcacatcta tctgctggat ctggaactga acgtgctgga tgaactggat
      541 ctgtgccagc cctacacgcc gggcgtttat caactgcacc ggcatgacga ccgccatatc
      601 agcttccgct ttttcgacga caggttgtgg tcactgcaga tccgcgagga gccccgcccc
      661 agcccgctca attattcgcg ttttcccacc cggcgcccgc tcaagctgtg gggacggcaa
      721 tacctggaac tgaaccgatc cgaatccagc gcggcctgac ccggctcagc gctcgccaag
      781 gacggcgcgg tacagctctg ccatcccggc atcgaaacag gcaaacacca cccgatcaat
      841 catcagaaac acttccagac agcgctccac ttcgtccacg gcaatcgcca cggcatcttc
      901 cctcggatag ccatacacac cacaactgat ggcgggaaac gcaatggaac gggcgccctc
      961 ccggacggcc agggtcatgc aggcgctgta acaggaacgc aactgctcag cttcaccctc
     1021 ggtgccgccg cgccagaccg ggccaacggt gtgaatgatg gtgcgcgcgg gaagggcaaa
     1081 gcccggtgtc accaccgcct gccccgtctc acaataaccg atgcggcggc aggcctcctg
     1141 caagcgctcc gcaccggcag cccggtggat ggcaccatcc accccgccgc caccggccag
     1201 ccggttgttg gcggcattga cgatggcgtc cactttcaga agagtgatat cgccgtgcca
     1261 gacttccatt ctgtcgtcca tgatcatgcg cgcaatcctg cgaccaacgc cgttatctag
     1321 aggctgtaaa gcagaaatcc cataatgcca agcaccagca gagctgcaat ggccatcgca
     1381 acacccagcc atgacagggc cggagaacct ttgtgcacct gaggcagcac gacaggctct
     1441 tcttccgcga gggaatccgg atgcggggca aaatcctcgc tcacgcaccg ttgcaggatg
     1501 tcacgacgcc ggcggcggta ttcacccttg ttgaattcgc catcgcaata ctggcgggcc
     1561 agttcgcgaa tttcctgcgc acattgggtt tccatttcgg aattcatcat tactggatat
     1621 ggaccgtgac cactgtgacg ttgtcccgcg cgccggcttt cagcgcgtct tcaatcagcg
     1681 cctgggtgca ggattcgaca tccggatttc tcagatggtg caccagtttg ccttcgctca
     1741 gcgcgccata caagccgtca ctgcacagca gatagacatc gcccttgcgc agctccacca
     1801 gggtcacatc cacgtacagg gtttcgctgg cgcccacggc gcgggtaatc acattcgagg
     1861 cgggatgatt cttggcttct tccggcgtga tcatgccgcg cgccagcatt tcgttcacct
     1921 ggctgtgatc gtcggagact ttctgcaatt tgtgatcgcg gtaacggtag aggcgggaat
     1981 cgccggccca caggcacacg ccgacagatt cacgcaccag caggctcacc accgtgctgc
     2041 ccatggtgcg gccttcgcag tgggtgcggg aataggtgcg gattttctgg tgcacgtcga
     2101 gcagcgaatc ttccagtgcg tcgaccacgt ctggcagcgg ctgctccaca tccagacggg
     2161 ccagtgtggc agtaatcatt tcactggcca cttcgcccgc gtcatggccg cccatgccat
     2221 cggcaactac ccagaggccg tcattgttgc ggttgagaaa agcatcttca ttcatgtttc
     2281 tcacggcgcc gcagtgggtg gcggcgctgc tgagccaatg ggtctgcacc atgcagtttt
     2341 cttctcgttc tggacaggat tgtggttatt gtgggggagc accggcaagg gtgcgatcgc
     2401 gttattccca atcccccgcc cgccgcgtgg agtggccctg cggcaacggc aaaaaagtgc
     2461 tgatcaggag cgctgacaga tccattcaga gcctgtgctc caccatagac tatagctggg
     2521 gaagcacagc agcagtaaac ggtgcagcaa ccggaatgcg gcatccatcg catcctgccc
     2581 tgccatccgg atgctgttca gccagcgctc aagatcaact atggtaacac cgtcgcgcct
     2641 tcctgccagc gccacccgtt ccaattcgcc aaaccattct ccttgtgcag ccggc
//
LOCUS       DASUEG010000336         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_223563, whole genome shotgun sequence.
ACCESSION   DASUEG010000336 DASUEG010000000
VERSION     DASUEG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223563"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VFX11_06295"
     CDS             <1..1279
                     /locus_tag="VFX11_06295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938266.1"
                     /translation="RLYLEFALFMAALILFHCHLRGISLRFFWPGQVQLNESVWLGSG
                     LLSIFFGVLFADHFLQLEQRGFPLLKLLRLTRWLALLAVFMAPWLGTELGVYGLVLLT
                     LMATLMLMISVFYHYQTDNRPVQLFAMGWVVLVGGVLILAGNRTGLLPVNLLTEHAVS
                     LATLAQFMLFSMALSHRVNHEKALSLQRGAQRLTALQEACALQAQQLRAVEMQRNADS
                     MALQLQTATNERLTCEIDDSGAALEQASQRLRELSRLDPLTGLYKRNHCNERLREEFE
                     RSARTGQPVSLILVSVDQFTQVCERWGVRAGDQLLRMVAEWIQAVTQHHCASLFQFEE
                     KVFAVLLPGVGADRASALAEMIRAAIGQQPFVQATTAQPVTVSAGVGTLVPQPNGRPE
                     SWVRLAQQAMNRARQQGGDAVCVETPPAGVAHG"
     gene            1272..3119
                     /locus_tag="VFX11_06300"
     CDS             1272..3119
                     /locus_tag="VFX11_06300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938267.1"
                     /translation="MAELRAVLLVLLAWLLPALASADHGRHWALLLDTGEYTDVVALD
                     QADVEWEASRWQVPNLGFQSRPWWFRLDIAPDELAPGTWRLWLHNSLLSDMTFAVLED
                     GKLKSSQRDGLWLRLDQRAAPFRQPAFAFTVEAGKRYRIYLRVHSETALQVPAQLQPE
                     DVFLAQKEAGDLLLGLFLGVLVAMMLYNLALYFSARETGFLLYGGHVLALLFFIVSWQ
                     GLGATWFWPGLIGLQSSSIAIATFLVIGFSTWFCCVFLQLNQDNFPYYRLFIAIRVFA
                     FGGVLVMPVLPIQWAVVSSSCLSVPAVVLVARSLLQREALRERPARLFAMGWALYVTG
                     AFMMGLNKFGFIEVTPASENLLLWASVFDMVLLCIALGDRHILDRNRQLERQAQALEQ
                     AGRELEGLQLRVQREQAARAALAQDLAGQESYGRELELRNRARQQEMAEVQRGLELAS
                     EADSLTGLKNRHYFADRLQEEIRRCRHLGTCLSLLLVDIDHFSQINDSHGHLAGDECL
                     RQTAEILRQQLKRPADILCRTGGEQFCVLLPDTGGDGALQLAEQMRMKVADCPMLCAG
                     VRVMATVSVGVLLYDPASGARTDQLLDLAGQALRQAKASGRNCVSLAAA"
BASE COUNT          515 a          913 c         1149 g          703 t
ORIGIN      
        1 tcggttgtac ctggaattcg cgctgttcat ggcggcgctg attttgttcc attgccatct
       61 gcgcggcatc agtctgcgct ttttctggcc tggccaggtt cagctgaatg agtcggtatg
      121 gctgggcagc ggtctgctgt cgatcttttt cggtgttttg ttcgcggatc atttcctgca
      181 actggagcaa cggggatttc cgctgctgaa actgctgcga ctgacccgct ggctggcgct
      241 gctggcggtg ttcatggcgc cctggctggg tactgaactg ggcgtctatg gcctggtgct
      301 gctgacgctg atggccacgc tgatgctgat gatctcggtg ttctatcact accagaccga
      361 taaccgcccg gtgcagctgt tcgccatggg gtgggtagtg ctggtggggg gtgtcctgat
      421 cctggcgggc aaccggaccg ggctgctgcc ggtcaatctg ctgaccgaac atgcggtgtc
      481 gctggccacg ctggcgcagt tcatgctgtt ttccatggcg ttgagccacc gggtcaatca
      541 cgaaaaggcg ctgtcgctgc agcggggcgc ccagcgtctg acggccttgc aggaggcatg
      601 cgcgttgcag gcccagcagt tgcgggcggt ggaaatgcag cgcaacgctg acagcatggc
      661 gctgcagttg cagaccgcta ccaatgagcg cctgacctgc gaaatcgacg acagtggcgc
      721 ggcactggaa caggccagcc agcgtttgcg cgaactgtcg cggctggatc ccctcaccgg
      781 cctgtacaaa cgcaatcact gcaatgaacg cttgcgggaa gaattcgaac gcagtgcccg
      841 taccgggcag cccgtcagcc tgatcctggt gagtgttgac cagtttaccc aggtctgtga
      901 gcggtggggc gtgcgtgccg gtgaccagtt gttgcgaatg gtggcagagt ggatccaggc
      961 ggtgacccag catcattgcg ccagcctgtt ccagttcgag gaaaaagtgt ttgctgtgct
     1021 gctgccgggg gtgggcgcgg atcgggccag cgcattggcg gagatgattc gcgcggccat
     1081 cggccagcag ccgttcgtgc aggccacgac agcacaaccg gtgaccgtga gtgccggcgt
     1141 gggcactctg gtgccacagc cgaatggccg gccggagagt tgggtgcggc tggcgcagca
     1201 ggcaatgaac cgggcccgcc agcagggtgg tgatgcggtc tgtgttgaga cgccaccagc
     1261 gggggtggcc catggctgag ttgcgcgcgg tgttgctggt gttgctggcc tggctgttgc
     1321 cggcgctggc gtccgctgat catggccggc actgggcgtt gctgctggat acgggtgaat
     1381 acacggatgt ggtggcgctg gaccaggccg atgtggaatg ggaggcgtcg cgctggcagg
     1441 tgcccaacct gggtttccag tcgcgaccct ggtggttccg cctcgacatt gcgccggacg
     1501 aactggcgcc gggcacctgg cgcctgtggt tacacaacag cctgttgtcg gacatgacct
     1561 tcgcggtgct ggaagatggc aagctcaaat cctcccagcg cgatggcctg tggctgcgac
     1621 tggaccagcg tgccgcgccg ttccgccagc cggcttttgc gttcacggta gaggccggca
     1681 aacgctaccg catttacctg cgcgtgcatt cggaaaccgc cctgcaggtg ccggcacaat
     1741 tacagccgga ggatgtcttc ctcgcgcaga aggaggccgg tgacctgctg ctggggctgt
     1801 tcctgggcgt gctggtcgcc atgatgctgt acaacctggc gctgtatttc agcgcgcggg
     1861 aaaccggctt tctgctgtat ggcggccatg tgctggcgct gctgtttttt atcgttagct
     1921 ggcaggggct gggcgcgacc tggttctggc ccggactgat cggcctgcag agcagcagca
     1981 tcgcgatcgc cacctttctg gtgatcggtt tttccacctg gttttgctgc gtgttcctgc
     2041 aactgaacca ggacaacttc ccctactacc ggttgttcat cgcgatccgc gtgtttgcct
     2101 ttggtggcgt gctggtgatg ccggtgctgc cgatccagtg ggcggtggta agttcgtcgt
     2161 gcctgtcagt gccggcagtg gtactggtgg cgcggtcgct gttgcagcgg gaggcattgc
     2221 gcgaacgtcc cgctcgtctg ttcgccatgg gttgggcgct gtatgtcacc ggcgccttca
     2281 tgatggggct gaacaaattc ggatttatcg aagtcacgcc ggcgtcggaa aatctgttgc
     2341 tgtgggcatc ggtgttcgac atggtgctgc tgtgcatcgc gctgggcgat cgccatattc
     2401 tggaccgcaa ccggcaattg gagcggcagg cacaggcgct ggagcaggcg ggccgtgaac
     2461 tggaaggatt gcagttgcgt gtgcagcgtg aacaggcggc gcgggcggcg ctggcgcagg
     2521 atctggcggg acaggaaagt tatggtcgtg aactggagct gcgcaatcgc gcgcgccagc
     2581 aggaaatggc tgaggtgcag cgcggactgg agctggccag tgaggccgac agcctgaccg
     2641 gattgaagaa ccgccactat tttgctgacc gcctgcagga ggagatccgc cgctgccgtc
     2701 atctgggcac ctgcctgagc ctgctactgg tggacatcga ccatttcagc cagatcaacg
     2761 acagtcatgg ccatctggcg ggggacgaat gcctgcgtca gactgccgag atcctgcgtc
     2821 agcaactgaa acgtcccgcc gatatcctgt gtcgtaccgg cggcgagcag ttctgcgtgc
     2881 tgctgccgga cacgggcggc gacggtgccc tgcaactggc ggagcagatg cgcatgaaag
     2941 tggcggactg ccccatgctg tgcgcgggtg tgcgggtgat ggcgacggtc agcgtcggcg
     3001 tgttgttgta tgatcccgcc agtggcgcgc gcacggatca gctgctggat ctggcgggcc
     3061 aggcgttgcg acaggccaag gccagcggac gcaattgtgt gagtctggcc gccgcttagg
     3121 tggaccggcg tcaatgcttc ggcgcattcg cctgtgccgg agcagtttta cgtgccagat
     3181 tcatgcgata ttaatatgat gcccgctgat tggtaaaaag gagcttgccc atgcctgttc
     3241 gaacgttgct attgctgctc gcgctgctgg tttgtgcctg
//
LOCUS       DASUEG010000337         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1332106, whole genome shotgun sequence.
ACCESSION   DASUEG010000337 DASUEG010000000
VERSION     DASUEG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332106"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFX11_06305"
     CDS             <1..327
                     /locus_tag="VFX11_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938268.1"
                     /translation="LGAFAGGAGGGYLLGAYGVGAVFGLAAGLVALWLLVAAGMKQPR
                     KLQDYTLQLDSINAAESATLARRLLGVSGVEDAVVIPHERAAYLKVDNQCLDKEQLRE
                     VASAAH"
     gene            367..855
                     /gene="ssb"
                     /locus_tag="VFX11_06310"
     CDS             367..855
                     /gene="ssb"
                     /locus_tag="VFX11_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX4938269.1"
                     /translation="MAQRGVNKVILVGNLGQDPETRYLPSGGAVTNVTIATSETWKDK
                     NSGQMQERTEWHRVVFFNKLGEIAGEYLKKGSKVYIEGALRTRKWQDKDGHDRYTTEI
                     VANEMQMLDSKGGSGGGNAGGGGFEGYDQLPEAQGGGASSYQNSSAPSPAGADFEDDI
                     PF"
     gene            995..1741
                     /gene="gspN"
                     /locus_tag="VFX11_06315"
     CDS             995..1741
                     /gene="gspN"
                     /locus_tag="VFX11_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX4938270.1"
                     /translation="MKTRSLILIGLLSFAFFVVALFPASLVWRLAGGALPGLPVTVEQ
                     VGGTVWSGFLQTRVQAPVPGPLLIHWDLKGLRLLMGELALSLRLEGAQYRLEGNGFWG
                     LWGKGVADVNGDVQAALLEQILNQYDISAQGAIKVNDISAKLSGQRVVSVGGNISWSG
                     GQVAVRNGPSPQNIDFPGVKGELAAVEGNLILAVTETRTNKPLGELSLLPEQGLAGVK
                     VLKRVLSLAGFGGEGEEDKTLLNLQQPLPF"
     gene            1931..2899
                     /locus_tag="VFX11_06320"
     CDS             1931..2899
                     /locus_tag="VFX11_06320"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX4938271.1"
                     /translation="MATDATQLNTWVLRANALACAGLAVAIAYVLVRMVYLFVDTSSG
                     NMPPAVYGTAVSSASSQMSPQRRIDAGAIALWHLFGEEGVKPVAQAQNQDVVAPPTRL
                     ALELQGVFVAPKEEDSTAMISESRKESQLYRIGSKVAGSATLTAVYPDRVLLSINGKL
                     EALYFPDNKVGGAGPGMVRSAGPIPGAAPVRSVSARTGRVSEMPRPGMGGASRPGGGA
                     MPGVEQAQEVINSLQAEISANPDAVLGQFGLATNNGRGYKVTDSSNPAFAAIGARPGD
                     VLVSVNGRALGDPQTDVNLIKDVMESGCAKVVGERDGRQYSAEACP"
BASE COUNT          640 a          799 c         1016 g          610 t
ORIGIN      
        1 ctgggtgcct ttgccggtgg tgcgggaggc ggctatctgc ttggcgccta cggtgtcggt
       61 gcggtatttg gcctggcggc gggcctggtg gcgttgtggt tactggtggc agccggcatg
      121 aagcagccac gcaagttaca ggactatacg ctgcagctgg acagcatcaa cgccgctgaa
      181 tccgccacgc ttgcccggcg tctgctgggc gtcagcgggg tcgaggatgc ggtagtaatt
      241 ccgcatgaga gagccgccta tttaaaggtg gacaatcagt gtctggataa agaacaatta
      301 cgggaagtgg cttcggccgc ccattaaaca cgcgaaaatt aggtcgaatg ccgacaggag
      361 aggaacatgg cgcaacgtgg ggtcaacaaa gtcattctgg tgggcaatct gggacaggat
      421 cctgagaccc gttacctgcc cagcggtggc gccgtgacca atgtcaccat cgctaccagt
      481 gagacctgga aagacaagaa cagtggccag atgcaggagc gcaccgagtg gcaccgcgtg
      541 gtgttcttca acaagctggg tgagatcgcc ggcgaatatc tgaagaaggg ttccaaggtc
      601 tacattgaag gcgccctgcg tacccgcaag tggcaggaca aggatggcca tgatcgttac
      661 accacggaaa tcgtggcaaa cgaaatgcag atgctcgaca gcaagggtgg ttccggtggc
      721 ggcaatgccg gcggcggtgg cttcgaaggc tacgaccagc tgcccgaagc acagggaggc
      781 ggtgcttcca gttaccagaa ctcctcggct ccatccccgg ccggtgcgga tttcgaagac
      841 gatattccgt tctgaccggt ttgccatctc catcgtgcag ccctgtaaca gcaggagaag
      901 agagctggcg aaattatgct atgctcccga cttctgctta aactaataag aataagatga
      961 agtcagggag cgggacgatc ccctttaact ctccatgaaa acccgaagtc tgattctaat
     1021 aggactgttg tccttcgcat tctttgtcgt tgccctgttc cctgcaagcc tggtgtggcg
     1081 gctggcgggt ggtgccttgc ccggtctgcc ggtaacggtt gagcaggtgg gtggcactgt
     1141 ctggagcggg tttctgcaaa cccgggtaca ggctcctgta cccggcccgc tgctgattca
     1201 ctgggatctc aaggggctgc gtctgttgat gggcgagctg gctctgagcc tgcgccttga
     1261 aggagcgcaa taccgccttg agggcaatgg tttctggggg ttgtggggca agggcgtggc
     1321 cgacgtgaac ggtgacgtcc aggccgccct gctggaacag attctgaacc agtatgacat
     1381 cagtgcccag ggcgccatca aggttaatga tatctcggca aaactgtcgg gccagcgcgt
     1441 ggtatcggtg ggcggcaaca tcagctggag cggcgggcag gttgcggttc gtaacggacc
     1501 cagtccacag aacattgatt tccctggtgt gaagggggaa ctggctgcgg tggaaggcaa
     1561 tctgatcttg gctgtgactg agacgcgcac caataaaccg ctgggcgaac tgagcctgct
     1621 ccctgaacag gggctggctg gcgtgaaggt gttgaagcgc gtgctgagtc ttgccgggtt
     1681 tggcggagaa ggggaggaag acaaaacgct tctgaacctt cagcaaccac tgccgttcta
     1741 gacggattgc gcgcgcccat tgttaaggat tccggcgaaa ctgttgcgaa tgcgtacagg
     1801 ctattcgctc cgccagggtg atcagttaaa gtgcaaagca gccatacaag tgctacaaga
     1861 gcaaggtgct gcgaatgcac gaggcattca aaacagggcc ggcctgctgg ccggtgagta
     1921 ggagagagct ttggcaaccg atgctaccca attgaatacg tgggtattac gcgcaaatgc
     1981 gctggcctgt gccgggctgg cggttgcgat tgcctatgtg ctggtgcgaa tggtctatct
     2041 gttcgtggat accagtagcg ggaatatgcc acctgctgtt tacggtacag ccgtgtcctc
     2101 tgccagttcc cagatgtcgc cacaacgccg gattgatgct ggtgccattg ctctgtggca
     2161 cctgtttggc gaggaaggcg tgaaaccggt ggctcaggcc cagaatcagg acgtggtagc
     2221 ccctccgacc cggctcgcgc tggaattgca gggcgtgttc gtggcgccga aggaagagga
     2281 ttccaccgcc atgatttccg aatcccgcaa ggaaagccag ttgtaccgga tcggcagcaa
     2341 ggtggcgggc agtgccaccc tgacagccgt gtatcccgac cgggttctgc tcagtatcaa
     2401 cggcaagctg gaagcacttt acttccctga caacaaggtt ggtggcgctg gtccgggaat
     2461 ggttcgcagc gctggcccca taccgggtgc tgcgcccgtg cgttccgtca gcgcgcgcac
     2521 cggtcgtgtt tcggaaatgc cgcgtcctgg catgggaggc gcgtcccgcc cggggggagg
     2581 cgcgatgcca ggtgtcgaac aggcgcagga agtgatcaac agcctgcagg cggaaatcag
     2641 tgccaatcct gatgcggttc tgggccagtt cggcctggcc accaataacg gccgtggcta
     2701 caaggtgacg gattccagca acccggcctt tgcggcgata ggcgcccggc cgggcgatgt
     2761 gctggtgtcg gtgaacggac gggcgcttgg cgatccacag acagatgtga acctgatcaa
     2821 ggacgtgatg gaatccggct gtgcaaaggt ggtcggtgag cgcgacggcc gccagtacag
     2881 cgcggaagcc tgtccctgac aattcggaat acagtaattc tggaagaata aaaatgaacc
     2941 taggtttggt tgcaagcgag ttcaacatga aactgaagcg aatgctcggc atggggctgg
     3001 cgatagcggc gctgtctctg gcaaccgttg catcggccca gcagcagggg cagggcagct
     3061 ggaag
//
LOCUS       DASUEG010000338         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1417930, whole genome shotgun sequence.
ACCESSION   DASUEG010000338 DASUEG010000000
VERSION     DASUEG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417930"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFX11_06325"
     CDS             <1..878
                     /locus_tag="VFX11_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4938272.1"
                     /translation="MTWLIATPAIAFILTLLFVRQLNRPLKRLANAARRIGRGEIVSN
                     LTQETGPREIQAVNKAFMQMSKDLQQASRERSLLLAGISHDLRTPLTRMRLTAELLGE
                     SEFTEGMIRDIEDMNAILNQFIMFVRDGSDEKTETGDLNEVIVEVVSQFESDGAVLRI
                     DLHPVPPILLKRLSLKRMFANLIGNAIRHGGGEVDVSCAMADSEIRVIVADRGPGLAE
                     QNLQELFQPFARGDQSRSTKGSGLGLAIVKRVVDMHHGRVQLRNREGGGLEAVVHLPL
                     TGELVPPDSLLQGMR"
     gene            complement(898..1539)
                     /locus_tag="VFX11_06330"
     CDS             complement(898..1539)
                     /locus_tag="VFX11_06330"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4938273.1"
                     /translation="MTSLLNLEALRKAQVQTDPFPYFTVDESILPDAVMDVIRDFPPI
                     RDGGSFNLDDVKYGPHFDRLLKCLDTPEFRKILCDKFDVNVMDLPMMITLRGYSRQKD
                     GRIHTDSKTKVLTILIYLNEKWTAETGKLRILRSKDNMDDYVAEINPGPGSLLAFKVT
                     DNGWHGYPAFEGKRQSIQINFLTGGAASSKHKFFHGLSAKLKKLRRKGNDDDY"
     gene            complement(1685..>2134)
                     /locus_tag="VFX11_06335"
     CDS             complement(1685..>2134)
                     /locus_tag="VFX11_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HEX4938274.1"
                     /translation="VENGLIQGFGMGFVFVPLSALAYSTLAPHHRNDAAAVFSLIRNI
                     GSSVGISVVTTLLARNIQINHAELGSHLVNYGAQGNLLDSATDQLSASGETSNSLLML
                     DSMVNSQAATIAYLNDFRLMMMVVLLVIPLLIFLRRTAAPAAGNTAP"
BASE COUNT          388 a          598 c          715 g          433 t
ORIGIN      
        1 tcatgacctg gctgatcgcc acgcccgcca tcgcgttcat tctgacattg ctgttcgtgc
       61 gccagctgaa ccggccgctg aaacggctgg ccaacgcggc gcggcggatc ggacgcggcg
      121 agatcgtcag taacctgacc caggaaaccg gtccgcgtga gattcaggcg gtaaataaag
      181 ccttcatgca gatgagcaag gatctgcagc aggccagtcg cgaacgttca ttgctgctgg
      241 cgggtatctc ccacgatctg cgcacgccgc tcacccgcat gcggctgacg gcggagctgc
      301 ttggcgaatc ggaattcacc gaaggcatga tccgcgatat cgaggacatg aatgccattc
      361 tcaaccagtt catcatgttc gtgcgtgacg gcagtgacga aaaaaccgaa accggcgatc
      421 tgaatgaagt gatcgtggaa gtggtgtcgc agtttgaatc cgatggcgcg gtgctgcgca
      481 tcgatctgca tccggtgccg ccgattctgc tgaagcggct gtcgctcaag cgcatgttcg
      541 ccaacctgat cggcaatgcg atccggcatg gcggtggcga agtggatgtg tcgtgtgcga
      601 tggcagacag tgagatccgc gtgatcgtgg cggatcgcgg cccgggcctg gcggagcaga
      661 atctgcagga actgtttcag ccgtttgctc gtggggacca gagccgctcc accaagggat
      721 cggggctggg gctggcaatc gtcaagcggg tggtggacat gcatcacggc cgcgtgcagc
      781 tgcgcaatcg cgaagggggc gggctggagg cggtggtaca tctgccgctg accggggagc
      841 tggtgccccc cgattcactg ctgcagggca tgcgctgaga acgcccctga cgttctctca
      901 ataatcgtcg tcatttcctt tgcgacgcag cttcttcagc tttgcgctca ggccgtggaa
      961 aaacttgtgc ttgctgctgg cggcgccacc ggtgaggaag ttgatctgga tcgactggcg
     1021 cttgccctcg aatgccgggt agccgtgcca gccgttgtcg gtcaccttga aggccagcag
     1081 cgaacccggg cccggattga tctccgccac atagtcatcc atgttgtcct tgctgcgcag
     1141 aatccgcagc ttgcccgtct ctgccgtcca cttctcattc agatagatca gaatggtgag
     1201 caccttggtt ttcgaatcgg tgtggatgcg gccgtccttc tggcgggagt agccgcgcag
     1261 ggtgatcatc atcggcaggt ccattacgtt cacgtcgaac ttgtcgcaca ggatcttgcg
     1321 gaattccggg gtgtccaggc acttgagcag gcggtcgaaa tgggggccgt atttcacgtc
     1381 gtccaggttg aagctgccgc cgtcgcggat cggcgggaaa tcgcggatca cgtccatcac
     1441 ggcatcgggc aggatggatt cgtccacggt gaagtagggg aagggatcgg tctggacctg
     1501 ggctttgcgc agggcttcga gattcagaag gctggtcatg gtcgtttatt tccggcagat
     1561 cggggtggtg aaatttggtg gtggtggcgc ttgatggagc ggaacatacc actttttgcc
     1621 ccggtgaacg agccggagcc ggcccggtgc tgaccggggt caatggccgg atggcgtgct
     1681 gccttcaagg cgccgtgttg cccgcggccg gcgcggcggt ccggcgcaga aagatcagca
     1741 gggggatgac cagcaatacc accatcatca tcaggcggaa gtcgttcagg taggcaatgg
     1801 tagccgcctg gctgttcacc atgctgtcca gcatcagcag gctgttgctg gtttcgccgc
     1861 tggcgctgag ctggtccgtg gcggagtcca gcaggttgcc ctgggccccg taattcacca
     1921 gatgcgaccc cagttccgcg tgattgatct ggatattgcg cgccagcagg gtggtgacca
     1981 cggaaatgcc cacactgctg ccgatgttgc ggatcaggct gaacacggcg gcggcgtcgt
     2041 tgcggtggtg cggggccagg gtggagtagg ccagcgcgct cagcggcacg aaaacaaagc
     2101 ccatgccgaa gccctggatc agcccgttct cgac
//
LOCUS       DASUEG010000339         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1928810, whole genome shotgun sequence.
ACCESSION   DASUEG010000339 DASUEG010000000
VERSION     DASUEG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928810"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1142)
                     /locus_tag="VFX11_06340"
     CDS             complement(150..1142)
                     /locus_tag="VFX11_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016139102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX4938275.1"
                     /translation="MPILLLAPMEGLTDFHMRDVLTRLGHYDWCVTEFIRVTDRVHPA
                     RVFFHACPELHTGGLTAAGTPVHVQLLGSDANMLAANAARAVELGALAIDLNFGCPAK
                     TVNNHGGGAVLLDTPETVHAIVRAVRAAVPADVPVSAKMRLGVKDRSRMIENAQGIEA
                     AGAQWLTVHARTKEDGYRPPAHWEAIAEIRSRVQMHVIANGEIWTPDDAVRCREVSGC
                     DDLMLGRGAVTRPDLVNRIRTAAPLLSWTEMVQAQQAFLDHMEAARRVGALPSPGDLE
                     QEPARWTERGAIGRYKQWLGMLTRGYVEAVPLFDQVKRMKSLNEIRALLNGRET"
     gene            1299..>2103
                     /locus_tag="VFX11_06345"
     CDS             1299..>2103
                     /locus_tag="VFX11_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX4938276.1"
                     /translation="MTARSLKASQAHATENDPRWAQVLARDPAADGQFFYSVASTGIY
                     CRPSCGARHPRPENVRFHFTREEAEAAGFRPCKRCKPDQPTLAARQAAAVAHACRCIE
                     AADTPLSLDALAKDLGMSPHHFHRLFRQHTGLTPHEYAAAHRANRVRQQLVQQSTVTE
                     ALVEAGFNSASRFYEKSDEVLGMTPQQFRHGGAQAIIRFAIGQCSLGAILVAQSERGI
                     CAILMGDDPDILAHDLEDRFPQATLVGGDAAFEKVVAQVIGFVEAPAIGL"
BASE COUNT          433 a          708 c          583 g          379 t
ORIGIN      
        1 ggcgcgaagc acgacattga gcaccggcgt atcaaacacg gtgtgacggg aatactgcat
       61 ggaaccctca gaaaactgtt aggtgagccg ctcgcgaccg gagggcatat cgattcatcc
      121 atgccgggcg cagtgtacag acttgccagt caggtttcac gcccgttcag cagcgcccga
      181 atctcgttca gcgacttcat gcgttttacc tgatcaaaca atggcaccgc ttcgacatac
      241 ccccgcgtca gcatgcccaa ccactgcttg tagcggccaa tggcgccgcg ctcggtccat
      301 cgtgccggtt cctgttcaag atcgccgggg gagggcagag caccgacacg acgagccgct
      361 tccatatgat ccagaaaagc ctgttgtgcc tgaaccatct ccgtccagga aagcagcggt
      421 gctgcggtgc gaatccggtt caccagatcc ggccgcgtga ctgcccctcg ccccagcatc
      481 aggtcgtcac acccggaaac ctcgcgacag cgcacggcat cgtctggcgt ccagatttca
      541 ccattggcaa tcacgtgcat ctgcacacgg gaacggattt ccgcaatcgc ctcccagtgc
      601 gcgggcgggc ggtagccatc ctccttggta cgcgcatgca ccgtcagcca ctgcgcaccg
      661 gccgcttcaa tgccttgcgc attctcgatc atgcgcgaac gatccttcac ccccaagcgc
      721 atcttcgcac ttaccggcac atcggccggc acggcagcac gcaccgcccg cacaattgca
      781 tgcacggtct ccggcgtgtc cagcagcacg gcaccaccac catgattgtt caccgtcttc
      841 gccgggcaac caaaattcag gtcgatggcc aatgccccca actccaccgc gcgcgccgca
      901 ttcgccgcca gcatgtttgc atcgctgccc agcaactgca catgcaccgg cgtaccagcg
      961 gccgtgagtc cgcccgtgtg caactccgga caagcatgaa aaaacacccg cgccggatgc
     1021 acacgatccg tcacgcgaat gaactccgtc acgcaccagt cgtagtgccc cagacgcgtc
     1081 agcacgtcac gcatgtggaa gtcagtcagg ccttccatgg gggcgaggag aaggatgggc
     1141 atcaaggatg tgacagggtg gcggatgatg accggcattc tagcgcggac actccgccga
     1201 tacaggccgg acgaggccga ttcaataaca agattcggag tgtcggccga atgggcaggg
     1261 ggtaccattg ttctgttgcc aacgaaaaga ctcgccagat gaccgcccga tcactcaagg
     1321 cctcccaggc ccacgccacc gagaacgacc cgcgctgggc gcaggtgctg gcgcgtgatc
     1381 cggcagccga tggccagttc ttctattccg tagccagcac cggcatttac tgccgaccgt
     1441 cctgcggcgc acgtcatccg cgaccggaaa atgtccgctt ccatttcacg cgggaagagg
     1501 cagaggctgc cggctttcgt ccctgcaagc gctgcaaacc cgaccagccc acgctcgcgg
     1561 ctcgtcaggc ggccgccgtt gcacacgcct gtcgctgcat cgaggctgcc gatacgcctc
     1621 tgtcactgga tgcactggcc aaagaccttg gcatgagccc gcatcatttt catcgcctgt
     1681 tccggcaaca caccggcctc actccgcatg agtatgcggc agcccatcgc gccaaccggg
     1741 tacgccaaca actcgtgcag cagtccactg tgacagaggc ccttgtcgaa gccggcttca
     1801 attccgccag ccgtttttat gaaaaatcgg atgaggtact gggcatgaca ccgcaacagt
     1861 tccgccacgg cggtgcacaa gccatcatcc gatttgccat cggtcagtgt tcactgggcg
     1921 ccattctggt ggcacaaagt gaacgcggca tttgcgccat cctcatgggt gatgatcccg
     1981 atatcctggc tcacgatctg gaagaccgat tcccccaggc caccctcgtt ggcggtgatg
     2041 ccgcgtttga aaaagttgtc gcgcaagtca taggctttgt tgaggcaccg gccatcggac
     2101 tgg
//
LOCUS       DASUEG010000340         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_510468, whole genome shotgun sequence.
ACCESSION   DASUEG010000340 DASUEG010000000
VERSION     DASUEG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510468"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..484)
                     /locus_tag="VFX11_06350"
     CDS             complement(293..484)
                     /locus_tag="VFX11_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938277.1"
                     /translation="MQPELTLNLKCPACGALTPKTLSCIKAQKAFDCDCGFHADLQPG
                     VKPRTQRNAGRKGKVAATA"
     gene            complement(587..1468)
                     /locus_tag="VFX11_06355"
     CDS             complement(587..1468)
                     /locus_tag="VFX11_06355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX4938278.1"
                     /translation="MASHQPGILAPLPPVARYLVFTHKSDTDPRPLLQLLAQQVDGER
                     IVAGLGQSLLQLCQAKIHGLHEMPALSGAAVEVPSTPASLWLWLRGEDRGELVNLGRK
                     LKSLLAPAFELRDAIDSYTYRRNRDLSDYEDGTENPADAAAFDAAIVQNELPGYEGSS
                     FVAVQQWLHNLNHFESLAQKERDYIVGRRQSDNEELKNAPESAHVKRSAQESFTPPAF
                     MVRRSMPWNDATREGLVFVAFGHTLQAFEAVMRRMVGLDDGITDGLFRFSQPVNGAYF
                     WCPPLRDGKLDLSLLKL"
     gene            complement(1507..4194)
                     /locus_tag="VFX11_06360"
     CDS             complement(1507..4194)
                     /locus_tag="VFX11_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938279.1"
                     /translation="MTIRDAAMIALNFRQKMMWLCGMGIVAVLIVGSVGYFSQVKEAT
                     AVSDSVVISQALRNHLEGDMMHDALRADVLAALLAAADRQRAEQQDQVVKDFTEHAQW
                     FRRSIDANRALPLNGEITKALEEAIPALDAYILEAGNIIQLAFENVGEAKARQPAFLE
                     RFEQLEGKLESVSDLISEGMEQSEKNLHADLLFYQALTAAFLVLAAVLILLVGTRIIR
                     GILQQLGGDPAYAVEVVRRVARGELDVQIRRDADDRDSLLSAIDDMRQKLKDSFDDIK
                     RIGEQTRQVAEESLRVRQALDVCDTAALIVQPDGIVIYCNQAALRLFASAENALVGAG
                     IRISARQLPGWDVNQLLRLIGQPLQGLRESAKHRLVVAGLTFDVVMTPVINADHSVTG
                     YVLEWLDLTELLARQDVERRVAQENARVRQALDNVTANTMIADADGNIVYVNAAVSSM
                     MRAAETDIRKALPHFSSDRLVGASFDSFHKNPSHQRNLLQHLNDTYRTMIEVGGRTFA
                     LVANPILNAERQRIGTVVEWTDRTQEVAIENELDALLRAANEGDLTRRLDLAGKQGFF
                     LKLSAGLNQLVGVAEGVIANSIRVMDALAHGRLNERIDNDYRGMFDKLKQDTNATVDI
                     LVNVVEQITESARAVEKGTQEIAQGNMDLSQRTEEQASSLEQTASSMEELLSAVKQSS
                     ENAKNANGVAAEARDRAGKGGDVVARAVAAMSTISASSKKIADIIAVIDGIAFQTNLL
                     ALNAAVEAARAGEQGRGFAVVAGEVRHLAQRSASAAKEIKELIRDSVDKVEDGTRLVN
                     ESGQTFNDLVTAVRNVSGIVQQIASAATEQESGIAQVNTAVAQMDQMTQQNAALVEQA
                     SAAGETLADQAGHLLKLIAFFDIGQQQTGTPRCPSPARM"
     gene            complement(4445..>5584)
                     /locus_tag="VFX11_06365"
     CDS             complement(4445..>5584)
                     /locus_tag="VFX11_06365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4938280.1"
                     /translation="IVELTNRYYLQDDASVLPRNIATRKAFENAISLDIAMGGSTNTI
                     LHLMAAAQEGEVDFGMKDIDRLSRKVPQLCKVAPNTQKYHIEDVHRAGGIMSILGELD
                     RGGLLHTDRPTVHSTTLKDALEQWDIKRTSNADVQMFFKAGPAGIPTQVAFSQDCRWD
                     SLDDDRAEGCIRSVEHAYSREGGLAVLHGNIAPDGCVVKTAGVDDSILVFEGPARIFE
                     SQDAAVKGILNDEVKAGEVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKVCALLTDG
                     RFSGGTSGLSIGHVSPEAASGGAIALVEAGDLIRIDIPNRTIDVKVSDEVLAQRRAAM
                     NARGKDGWKPAHPRERKVSTALKAYAALATSADRGGVRDLSQLEK"
BASE COUNT         1057 a         1755 c         1616 g         1156 t
ORIGIN      
        1 cttgagtaca tcagcgtggt tgccggcgtg gtagccgtgg cgataactca gcatgcgaat
       61 tccagtgggt tggacgggac aaggacgggg cgacgcagag ggccgctggc cggggctgtg
      121 ctggacatga atacaatctg gaatgagtga aacaagaggg caagcaggct aacacaggtt
      181 gccggggatg aaaacgccgg catcacctgc aggcgggcgg ctttttgcgg ggagcagtac
      241 ccgcccgggg cgcccggccc cgtcgctggg cagatcacgt ggccgccggt aatcaggctg
      301 tcgcggcgac cttgcctttg cggcccgcgt tgcgctgggt acgcggcttt accccgggct
      361 gcaggtcggc gtgaaaacca cagtcgcaat cgaaggcttt ctgggccttg atgcaggaca
      421 gggtcttcgg ggtaagggca ccgcaggcag ggcatttcag attcagcgtc agttcgggtt
      481 gcatgagcat ctccctttcg tctttcgact tcttgttgtg gactgaacgg ctttttacag
      541 aactgcgttg ataaatctag caggcgcctg cacaaaagag aagcaatcag agcttcagca
      601 gcgacaggtc cagttttccg tcccgcaacg gcggacacca gaaataggca ccattgaccg
      661 gttgcgagaa acggaacagg ccgtcggtaa tgccgtcgtc gagccccacc atgcgccgca
      721 tcaccgcctc gaacgcctgc agggtatggc cgaatgccac gaacaccaga ccttcccggg
      781 tggcgtcgtt ccagggcatc gagcgccgca ccatgaaggc cggcggcgtg aagctttcct
      841 gggcagagcg tttgacgtgg gcggattccg gcgcgttttt cagttcctcg ttgtcggact
      901 ggcggcgacc gacaatgtag tcacgctctt tctgcgccag ggattcgaaa tggttcaggt
      961 tgtgcagcca ctgctgcacc gcaacaaaac tggagccctc gtagccgggc agttcgttct
     1021 gcacgatagc ggcatcgaac gcagcagcat cggcaggatt ttcggtaccg tcctcataat
     1081 cggacagatc gcgattgcgc cggtaggtgt agctgtcgat cgcatcgcgc agttcaaacg
     1141 cgggggccag cagggatttc agcttgcggc ccagattcac cagttcaccg cggtcctcgc
     1201 cccgcagcca cagccacagc gacgccggcg tggacggcac ttccaccgcc gcgccactca
     1261 gcgccggcat ctcgtgcagg ccgtgaatct tggcctgaca cagttgcagc aggctttgtc
     1321 ccagaccagc cacgatgcgc tcgccgtcta cctgttgcgc cagcagctgc agcaatgggc
     1381 gcggatcggt gtcggattta tgcgtgaata ccagataacg ggcaacgggt ggcaacgggg
     1441 ccagaatgcc gggttgatgt gacgccatta caacagtcct gtcagcgatt gtatggattt
     1501 tttatatcac atccgcgccg gtgaaggaca cctcggcgta ccggtctgct gctgaccgat
     1561 atcaaagaac gcaatcaact tcagcagatg cccggcctga tctgccagag tttcaccggc
     1621 ggcagacgcc tgctccacca gcgcggcgtt ctgctgcgtc atctggtcca tctgcgcgac
     1681 ggctgtattg acctgcgcaa tgccggattc ctgctccgtc gccgcactgg caatctgctg
     1741 cacgatgccg ctcacgttgc gcaccgccgt caccagatca ttgaacgtct ggccggattc
     1801 gttgaccagc cgggtaccgt cttccacctt gtcgacgctg tcgcggatca gctccttgat
     1861 ctctttcgcc gcactggcac tgcgctgtgc cagatgacgg acttcgccgg ccaccaccgc
     1921 gaaaccacgg ccctgctcgc ccgcccgtgc cgcttccacc gccgcattca gcgccagcag
     1981 gttggtctgg aatgcgatac cgtcaatcac ggcaataata tcggcaattt ttttgctcga
     2041 tgcgctgatg gtgctcatgg ccgccacggc ccgcgctacc acatcaccac ccttgccggc
     2101 ccgatcgcgc gcctcggccg ccacgccgtt ggcatttttt gcgttctcgc tgctttgttt
     2161 cacggcgctg agcaactctt ccatgctcga cgccgtctgc tccagcgacg acgcctgctc
     2221 ttcagtacgc tggctgagat ccatattgcc ctgggcaatt tcctgggtac ctttttccac
     2281 cgcgcgggcc gactcggtaa tctgctccac cacgttcacc agaatatcca cggtggcatt
     2341 ggtgtcctgc ttgagcttgt cgaacatgcc gcggtaatca ttgtcgattc tttcattgag
     2401 ccggccatga gccagtgcat ccatcacgcg gatggaattg gctatcacgc cttccgccac
     2461 gcccaccagc tggttcagcc cggcgctgag cttgaggaaa aaaccctgct tgccagccag
     2521 gtcgagtcgc cgggtcagat caccctcatt ggctgcccgc agcagcgcat ccagttcgtt
     2581 ttcgatggcc acctcctggg tgcggtcagt ccattccacc acagtgccga tgcgctgccg
     2641 ctcggcattg aggataggat tggccaccag ggcaaacgtg cgtccaccca cttcgatcat
     2701 ggtgcggtag gtatcattca gatgctgcag cagattgcgc tggtgtgacg ggttcttgtg
     2761 gaaactgtca aaactggcgc ccaccagacg atcggaactg aaatggggca aggctttgcg
     2821 gatatcggtt tccgccgctc tcatcatgct gctgaccgcg gcattgacgt acacgatatt
     2881 gccatccgcg tcagcgatca tggtgttcgc cgtcacattg tcgagcgcct ggcgcacgcg
     2941 ggcattttcc tgcgcgacgc gacgctccac atcctgccgc gccagcagtt cggtcagatc
     3001 cagccattcc aatacgtagc cggtgacaga gtggtccgca ttgatgaccg gtgtcatcac
     3061 cacgtcgaag gtgaggccgg ctaccaccag ccggtgtttc gccgactcgc gcagcccctg
     3121 cagcggttgc ccgatcaggc gcaacagctg gtttacatcc cagcccggca actggcgcgc
     3181 actgatgcga atgcccgcgc ctaccagcgc gttttcggca gatgcaaaca atcgcaacgc
     3241 tgcctgattg cagtagatga caatgccgtc gggctgcacg atcagagccg cggtatcaca
     3301 tacatcaagt gcctgacgca cccgcagtga ttcctccgcc acctgacggg tctgttcacc
     3361 aatccgtttg atatcgtcga aactgtcttt cagtttctgg cgcatgtcgt cgattgcact
     3421 gagcagactg tcacggtcgt cggcatcgcg ccggatctgc acatccagct caccccgcgc
     3481 cacgcgccgg accacttcca ctgcataggc tggatcccct cccagttgct gcaggatacc
     3541 gcggatgatg cgggtgccca ccaaaaggat cagcactgcg gccagcacca gaaaggccgc
     3601 tgtcaatgcc tggtaaaaca acagatcggc atgcagattc ttttcgctct gctccatgcc
     3661 ttcagagatc agatcactga cgctttcaag cttgccttcc agctgttcga aacgttcgag
     3721 aaaggctggc tgccgtgctt tcgcctcgcc gacattttca aacgccagct ggatgatgtt
     3781 gcccgcctcc aggatgtagg catccagcgc cggaatagcc tcctccagcg cttttgtgat
     3841 ttcgccattg aggggcagcg cccgattggc gtcgatactg cgccggaacc actgggcatg
     3901 ctcggtgaaa tccttcacca cctggtcctg ctgttccgcg cgctgacgat ccgccgccgc
     3961 cagcagcgct gccagcacat cggcgcgcag ggcatcgtgc atcatgtcgc cttcgaggtg
     4021 gttacgcagc gcctggctga taacgacgct gtcggaaacg gccgttgcct ctttcacctg
     4081 ggaaaaatag cccacactgc ccacgatcag cacagcgaca atacccatgc cgcacagcca
     4141 catcattttt tgcctgaaat tgagcgcgat catcgcagcg tccctgattg tcatccggtc
     4201 cattaagatt atttgaaaaa tcccggttgg ccagattttg actcaggccc acaggccggg
     4261 cgtgatgaca ggtttccaac ggtctgattt gactgagtaa aacgggaaat cgcgcaggaa
     4321 ttaaaaaagg ggaacagagc gaatcaggga agtgcgggat ttcgcctgca acaaaaagcc
     4381 cgcgccggac acgctgtccg acgcgggcct tcaaccccgc aggcccatgg cgggcccagg
     4441 cgttttattt ttccagctgc gacagatcgc gcacgccgcc acgatcggca ctggtggcca
     4501 gtgctgcgta ggccttcagc gcggtgctga ctttacgctc gcgcggatgg gccggtttcc
     4561 agccgtcctt gcccctggcg ttcatggcag cgcgacgctg tgccagcacc tcatcactga
     4621 ctttcacgtc gatggtgcgg ttgggaatgt cgatgcggat cagatcgccc gcttccacca
     4681 gcgcaatggc accaccggaa gcggcttccg gcgacacatg gccaatggac aggcccgagg
     4741 taccaccgga aaagcggcca tcggtcagca gcgcacacac cttgcccagc cctttcgatt
     4801 tcaggtagct ggtggggtac agcatttcct gcatgcctgg gccgcctttg gggccttcgt
     4861 agcggatcac caccacttca ccggctttga cttcgtcatt caggatgcct ttcacggcgg
     4921 cgtcctggct ttcgaaaatc cgcgccggac cttcgaacac cagaatgctg tcgtcgacgc
     4981 cggcggtttt caccacgcag ccatccggtg cgatattgcc gtgcagcacc gccagaccac
     5041 cttcgcggga ataggcgtgt tcgacggaac ggatacagcc ttcggcacgg tcatcatcca
     5101 gggaatccca gcggcaatcc tggctgaacg ccacctgggt ggggatgccg gcggggccag
     5161 ccttgaaaaa catctgcacg tcggcgttgc tggtgcgctt gatgtcccac tgctccagcg
     5221 cgtccttcag cgtggtggag tgcacggtgg gccgatcggt gtgcagcaga ccgccgcgat
     5281 ccagttcacc caggatgctc atgatgccgc cagcacgatg cacgtcttcg atatgatatt
     5341 tctgcgtgtt cggcgccacc ttgcacagtt ggggcacctt gcgcgacagg cggtcgatgt
     5401 ccttcatgcc gaaatccact tcgccttcct gcgcggcggc catcaggtgc aggatggtgt
     5461 tggtggaacc ccccatggcg atatccagcg agatcgcatt ttcaaatgcc ttgcgcgtgg
     5521 cgatattgcg cggcagcaca gaagcatcgt cctgcaggta atagcggttg gtgagttcga
     5581 cgat
//
LOCUS       DASUEG010000341         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1582187, whole genome shotgun sequence.
ACCESSION   DASUEG010000341 DASUEG010000000
VERSION     DASUEG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582187"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFX11_06370"
     CDS             complement(<1..231)
                     /locus_tag="VFX11_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX4938281.1"
                     /translation="MFARILIVIALACSFGAGNALATDYPSVEVVDAYLDLRTGPGRG
                     YPRVQIVERGDYIEIMAQKTDWFRVRTKKGFEG"
     gene            complement(332..>2479)
                     /locus_tag="VFX11_06375"
     CDS             complement(332..>2479)
                     /locus_tag="VFX11_06375"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938282.1"
                     /translation="QPALNLHVMNADGSCLPPPKQCIAQVTFNQSHDMYPIVMGNGKV
                     VYSRWDRFAGDNGIHLYEMNPDGTENQLLYGRHSHATGNNASALEFIRGNLMPDGGLF
                     VLARPNGTNYYGGDFLRIDTTGYVDNTQAVYGSGGTGPAQTTVTNRQILTDGSISPGG
                     YFSAFFPMWDNTNRALVSWSQCRVVIGATTQPCTQDNLATNGVVAAPPAYGLWVYNYQ
                     DNTQIPVVVAREGVVYTDVALGASRTRPSIIYDGDALTGVVEGKVSPETFSDMIAAEN
                     EEAILHIRSVYDFNGTARNPQNTAGVNLATLSNPSLSDADERPARFLRIIKAVSEPDR
                     DLKDPENGSFGNAGAQRMKEIIGYAPIEPDGSVKVRVPANVALSLSVLDANGKSLTQR
                     HNNWITLLPGEVMECKGCHASGNTGAALPPHGRYDAQAPSINSGRPQGSAYTGAVSTI
                     TSPFASATMAEARAAANEDIMELSADLLFTDVWTDTGSEAADAPVSLRYQDLPNYSAL
                     VSSQTTPANGNCSPWNSHCRIVINYNEHIQPMWDLDRGTLTTPILATDMQPATCIRCH
                     NAATMAANADADDYPPTQLELTNQSDVGGDVRVRSWFELFTTRPFQVAVGANLADCTS
                     VLIPVLDGNGDPVLDENGDPVTENVCPPGVTTTTPTMSSGGARASAAFFNKFEGAGSH
                     AGYLLPSELRLLAEWLDIGGQYYNNHFDAPDAN"
BASE COUNT          419 a          688 c          864 g          508 t
ORIGIN      
        1 gccttcgaaa ccttttttcg tgcgcacccg gaaccagtcc gttttctgcg ccatgatttc
       61 gatgtagtcg ccgcgctcga cgatctgcac gcggggataa ccccggccgg gaccggtgcg
      121 cagatccaga taggcgtcga caacttcaac ggagggatag tccgtggcca gggcattgcc
      181 ggcgccgaaa ctgcaggcaa gcgcgataac aatcagaatt cgggcaaaca tagggcgggc
      241 ggccggcctt tcgttggatt ttttaaggtg ttgttttttg ccgcattcta tagcatcgcg
      301 gcggggtggg ccacccgcgc cgcggatcgt gtcaattggc gtcgggggca tcaaaatgat
      361 tgttgtagta ctggccgccg atgtccagcc attctgcgag caggcgcagt tcggatggga
      421 gcagataacc ggcatgggaa ccggcgccct cgaacttgtt gaagaaggcg gcgctggcgc
      481 gggcgccacc ggaggacatg gtgggcgtgg tagtggtgac gccaggaggg cagacgtttt
      541 cagtgaccgg atcaccgttt tcatccagca ccggatcgcc gttgccgtcc agcaccggta
      601 tcagcacgct ggtgcagtcc gccagattcg caccaactgc cacctggaag ggccgggtgg
      661 tgaacagttc gaaccaggag cgcactcgca cgtcgccacc cacgtcgctc tggttggtca
      721 gctccagctg ggtgggagga tagtcatcgg catcggcgtt ggcggccatg gtggcggcat
      781 tgtggcaacg gatacaggta gcaggctgca tgtcggtagc cagaatcggt gtggtcagcg
      841 tgccacggtc cagatcccac atgggctgga tatgttcgtt gtagttgatc acgatccggc
      901 aatggctgtt ccagggggag cagttgccgt tggcgggcgt ggtctgcgac gacacgaggg
      961 ccgaatagtt gggcaggtcc tggtagcgca ggctgacggg tgcgtcggct gcttcgctgc
     1021 cggtgtcggt ccagacatcg gtgaagagca gatcggcgct cagttccatg atgtcttcgt
     1081 tggcggccgc gcgggcttcg gccatggtgg cactggcgaa cggtgacgtg atggtggaca
     1141 ccgcacctgt gtaggccgac ccctgagggc gcccgctatt gatggagggg gcctgggcgt
     1201 cgtaacggcc gtggggcggc agtgcagcgc cggtgttgcc gctggcgtgg cagcccttgc
     1261 attccatcac ctcgcccggc agcagggtga tccagttgtt gtggcgctgg gtgagggatt
     1321 tgccgttcgc gtccagcacg ctcagcgaca gtgccacgtt ggcgggcacc cgcaccttca
     1381 ccgaaccatc gggctcgatc ggggcgtaac cgatgatttc cttcatgcgc tgggcaccgg
     1441 cgttgccgaa agagccgttc tccggatcct tcagatcgcg gtcgggttcc gacactgcct
     1501 tgatgatgcg caggaaacgc gcgggacgtt catccgcgtc gctcagggac gggttggaca
     1561 gggtggccag gttgacgccg gcagtgttct gcggattgcg cgcggtgccg ttgaagtcgt
     1621 acacgctgcg gatgtgcagg atggcttcct cgttttctgc ggcaatcatg tccgagaagg
     1681 tttccggcga gactttgcct tccaccacgc cagtgagtgc atcgccatcg taaatgatcg
     1741 agggccgggt gcgggatgcc cccagcgcta catcggtgta gaccacgcct tcgcgtgcca
     1801 ccaccaccgg aatctgggtg ttgtcctggt agttgtagac ccacaggccg taggcgggcg
     1861 gtgctgcaac cacgccgttg gttgccagat tgtcctgggt gcagggctgc gtggtggcgc
     1921 cgatgacgac gcggcactgg ctccagctca ccagagcccg gttggtgttg tcccacatcg
     1981 ggaagaatgc agagaaatag ccgccggggg agatgctgcc gtcagtgaga atctgccggt
     2041 tggtgacggt ggtttgcgcc gggccggtgc cgccggagcc atacacggcc tgggtgttgt
     2101 ccacatagcc ggtggtatcg atgcggagga aatcgccgcc gtaatagttg gtgccgttgg
     2161 gacgggccag cacgaacagg ccgccgtcgg gcatcagatt gccgcgaatg aattccagcg
     2221 ccgatgcatt gttgccggtg gcgtgggagt ggcggccgta caacagctgg ttttcggtgc
     2281 cgtcgggatt catttcgtac aggtgtatgc cgttgtcgcc ggcaaagcgg tcccagcggc
     2341 tgtataccac cttgccattg cccatcacga tgggatacat gtcgtggctc tggttgaagg
     2401 tgacttgcgc aatacactgt ttgggtggcg gcaaacagga gccatccgca ttcatcacgt
     2461 gcaggttcag ggctggctg
//
LOCUS       DASUEG010000342         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2104525, whole genome shotgun sequence.
ACCESSION   DASUEG010000342 DASUEG010000000
VERSION     DASUEG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104525"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1640)
                     /locus_tag="VFX11_06380"
     CDS             complement(171..1640)
                     /locus_tag="VFX11_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938283.1"
                     /translation="MSGASLNLLDLKNPAIRTLHFSWMAFFITFVMWFGLGPLAAFIK
                     TEFALTEPQWKALLMLNVAITIPSRILIGILVDKFGPRAIYAGLLVISGIICIAFAWA
                     QDYTQLAIARFLMGFVGAGFVIGIRLVSEWFPARNVGLAEGVYGGWGNFGSAAAAALL
                     PLLASLFAPEYGWRFALTLAGVLSILYAFVFYHFVRNTPKGSTYFSPKKSGGLEVSSW
                     RDLWFYYALNVPLHLALVVLVWRLSPSNLNLLPQFWANAAYVVIVVLAVVQFRQIWQV
                     NAEHLRAGVPENQRFKFKQVSILSLSYFASFGSEIAVVSMLPIFFMETFGLSPVQAGF
                     STAGFVVANLIARPGGGWMSDRIGRRKALSIQVGGLAVGYALMSFIDSSWPLWVAIVI
                     TVANSFFVQAGCGGVYAVIPLIKRRMTGQIAGMAGAYGNVGGVIFLTVLSFVHASTFF
                     MVIAVCSAIVFLAVQFLDEPEGHMVEVLPDGTVQLIEVR"
     gene            1991..>3230
                     /locus_tag="VFX11_06385"
     CDS             1991..>3230
                     /locus_tag="VFX11_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4938284.1"
                     /translation="MKTRYAHGKPRLVLIGNGMAGIRTLEELLAIAPDRYEISVIGEE
                     HHGNYNRIMLSPVLAGEKNVDQIILNGDDWYAQHNVQLFKGEKAVQIDRIHRRIHTAS
                     GLVVPYDKLILATGSRPARIAVPGHDLNHILTFREIRDVNRMLQLSRQLRHVAVIGGG
                     LLGLEAANGLRQQGMQVTVVHSSNYLLNKQLDETAGRLLEKTLRAKGIDFCMNARTSR
                     ISGDEQGNARAVHFSDGRVLEADMMVLATGIVPNMELARDAGLQCGNGILVTDTLQTF
                     DPAIYAVGECVEHRGQLFGLVAPLYDQAKVCANHLAEMGIARYVQKAQATKLKITGIN
                     VFSAGNFLGDGESETITLHDRVLGHYKKLVLKNGRLDGVILYGDTQDGAWLFDLIQQR
                     RDVTAWRDQLIFGKDFCQDET"
BASE COUNT          781 a         1024 c          890 g          535 t
ORIGIN      
        1 cagaaccggc gcgtgcagat ggtgctgtcc gacaagggca aaggttacct taacccttcg
       61 cagcaataac gtcacctcga gaaaacggca gcggaataca gaattccgtt gccgttccgt
      121 ttccgctacc accccgcgta tgcggaaatc cgtttaccgg attttcacgt ctaccgaact
      181 tcaatcagct gcaccgtgcc atccggcagc acttccacca tatggccttc cggctcatcc
      241 aggaactgca ccgccaggaa cacaatggcg gaacagaccg cgatcaccat gaagaaagtg
      301 gatgcgtgca cgaacgacag tacggtgagg aagatcacgc cgccgacgtt gccgtaagcg
      361 ccggccatgc ccgcgatctg gccggtcatg cggcgcttga tcagggggat caccgcgtac
      421 acgccaccac aaccggcctg tacgaaaaag gagttggcaa cggtgatgac aatcgccacc
      481 cacaaaggcc agctgctgtc gataaaactc atcagcgcat agcccacggc caaaccgccc
      541 acctggatgc tcagcgcctt gcgacggccg atacggtcac tcatccagcc gccaccggga
      601 cgggcgatca gattcgccac cacaaatcct gccgtggaaa aaccggcctg caccggcgac
      661 agaccgaagg tttccataaa gaaaatcggc agcatggaca ccacggcgat ttccgatccg
      721 aacgacgcga aatacgacag cgacagaatc gacacctgct tgaacttgaa acgctggttc
      781 tccggcacgc cggcgcgcag atgctcggcg ttgacttgcc agatctggcg gaactgcacc
      841 accgccagta ccacgatcac cacataggcc gcgttggccc agaattgcgg cagcaggttc
      901 aggttgctgg gcgacaggcg ccacaccagc accaccagcg ccagatgcag cggcacgttc
      961 agcgcgtagt aaaaccacag gtcacgccag gaactcactt ccagaccgcc gctctttttc
     1021 gggctgaaat aagtggagcc tttcggtgtg ttgcgcacga agtgataaaa cacaaacgca
     1081 tacaggatcg acagcacgcc cgccagggtc agtgcgaaac gccagccgta ttccggtgcg
     1141 aacaaggatg ccagcaacgg cagcaacgca gccgcagcgg cggaaccgaa attaccccag
     1201 ccaccgtaca caccctccgc cagccccaca ttgcgggccg ggaaccattc cgataccagg
     1261 cggataccga tcacgaagcc ggcaccgaca aagcccatca ggaaacgcgc aatcgccagc
     1321 tgggtgtaat cctgcgccca ggcaaaggca atacagatga tgccgctgat caccagcagc
     1381 cctgcgtaaa tcgcacgggg accaaacttg tccaccagga tgccgatcag aatccgcgac
     1441 ggaatggtga tcgccacgtt cagcatcagc agcgctttcc actgcggctc ggtgagagcg
     1501 aattcggttt tgatgaaagc cgccagcggg cccaggccaa accacattac aaaggtaatg
     1561 aagaatgcca tccatgaaaa atgcagggta cggatcgccg gatttttcag gtccagcagg
     1621 ttcaggctag cgccactcat tggtttctcc ttgttgacag cacagggctc gctcccgccg
     1681 acaagtgcaa cccgtatgcc atcaccaaaa caggacaggg aaatagccca aatgccagat
     1741 cagggtgcag acattcgcgg gcaaaatgcg caaaagattc ataatggcgc gcgacacccc
     1801 ctcagtttgc accaatcact caccaaattg gttttgccaa cgccaccaga atgcacgtta
     1861 taagtgcatg ttggcgcaca gggctattcg ttatagtgca ttttgcccaa aaagcccttg
     1921 cacccgcctc gcctgcgcca ctggcacgcc acttgctcct gtccttgccg tggcagcgag
     1981 gagtaggcac atgaaaaccc gatatgcgca cggcaaaccc cggctggttc tgatcggcaa
     2041 cggcatggcg ggcatccgca cgctggagga actgctggcg atcgcgccgg accgctatga
     2101 gatcagcgtg atcggcgagg aacaccacgg caattacaac cgcatcatgt tgtcgccggt
     2161 gctggcaggg gaaaagaatg tggaccagat catcctcaac ggcgatgact ggtacgccca
     2221 gcacaacgtg caattattca aaggtgagaa ggcggtgcag atcgaccgca tccaccgtcg
     2281 catccatacc gcgtccggtc tggtcgtccc ctacgacaaa ctgattctgg ccaccggctc
     2341 acgtccagcc cgcatcgccg taccgggcca tgatctcaac catatcctga ccttccgcga
     2401 aatccgcgac gtgaaccgca tgctgcagct gagccggcaa ttgcgtcacg tggcggtgat
     2461 cggcggcgga ctgctgggtc tggaggccgc caacggcctg cgccagcaag gcatgcaggt
     2521 caccgtggtg cacagcagca attacctgct gaacaaacag ctggacgaaa ccgccggccg
     2581 cctgctggaa aaaaccttgc gcgccaaagg catcgacttc tgcatgaacg cccgtaccag
     2641 ccgcatcagc ggcgacgaac agggcaacgc ccgcgcggtc catttctctg acggccgcgt
     2701 actggaagca gacatgatgg tgctggccac cggcattgtt cccaacatgg aactggcgcg
     2761 ggatgccggt ctgcaatgcg gcaacggcat tctggtgacc gacaccctgc agaccttcga
     2821 tcccgccatc tatgcagtgg gcgaatgcgt ggagcaccgt ggccagctgt tcggcctggt
     2881 ggcgccgctg tatgaccagg caaaagtgtg cgcgaaccat ctggcggaaa tgggcatcgc
     2941 ccgttacgtg cagaaagccc aggccaccaa actgaaaatc accggcatta acgtgttttc
     3001 cgccggcaat ttcctgggtg acggggaatc ggaaaccatc accctgcacg accgcgtcct
     3061 cggccactat aaaaagctgg tattgaagaa cggacggctg gacggcgtga tcctttacgg
     3121 cgacactcag gacggcgcct ggctgttcga cctgatccag cagcgccgcg acgtcaccgc
     3181 ctggcgtgac cagctgattt tcggcaagga tttctgccag gacgaaaccc
//
LOCUS       DASUEG010000343         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_744610, whole genome shotgun sequence.
ACCESSION   DASUEG010000343 DASUEG010000000
VERSION     DASUEG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744610"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX11_06390"
     CDS             <1..377
                     /locus_tag="VFX11_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative solute-binding protein"
                     /protein_id="HEX4938285.1"
                     /translation="ALELYKGLGANGAIVNFALIQLTVQVIIRKSKFPEGYGQKSREF
                     VWSQYPRFMEYINSSTKSIKPSYWLEVPDADKPGYLEMFRQSRIKLRDQGFYDGKMLS
                     FLSKVRCQKDASLAECTAKDRE"
     gene            443..2515
                     /locus_tag="VFX11_06395"
     CDS             443..2515
                     /locus_tag="VFX11_06395"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4938286.1"
                     /translation="MSQSIVKRNLREWVSSFPVFLVLLLVILFSIGENFNAQVNRLGN
                     FLWPDYHLLRLDLPTPDCDPNIDVEARVQEVLNTATDDAFGDMFGAPDADTTRQSVQK
                     DLELCRLKHSEIERVNAQRTDGIAIFSSINMTLASLSQLSRDNTSNMLVLILFVCALT
                     ATLTYHQIGLRPMQTALDHRLGAVAQFVANGALSYSAYHYLINGWASGTVIQNEPIRW
                     SYMIGFGLLALVALVQVFKLPASVKPGGSFGKALLAIPLYCYMAFGSANYYILQHDNP
                     QGMALFVDKIMDTSSIYLSVGLYIWVGMLAKQTFLGEYVFRIFKPWKLPPELLAFVAV
                     VIMAIPTAYTGASGIIIIAMGAVVYTELRRVGARRQLALAATAMSGSLGVVLRPCLLV
                     LIIAVLNKEVTTDQMYGWGVKIFMLTALLFFLVSLVARQGPLRVAPVAEALMPSLQAF
                     RPLVPYALLFTLVAVIYAFGLDSYLDEHSAPYILPVIMLALLAYERLFGTPVEQRDDF
                     HDSERASTLEGSVRAATTNTTVHIGALLMLMCLGLTSGGVIEDSGIIQAATEQGSLFS
                     NIWVAMAALVLLLVFIGMIMDPFGVIILVSGAVAQAAYAQGIDPLHFWMVVLVAFELG
                     YLSPPVAVNHLLARQVVGEDEIEKAIDESKDGSFWIRHERYTLPLTVMSVSLLIVAFV
                     PLVYKNFF"
     gene            2564..3424
                     /locus_tag="VFX11_06400"
     CDS             2564..3424
                     /locus_tag="VFX11_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX4938287.1"
                     /translation="MSNLLDELVHLLDLETIEHNLFRGQSRDVGGRSVFGGQVVAQSL
                     VAAMRTLTEKRQCHSLHAYFLRPGDIETPIVYEVDRIRDGGSFTTRRVVASQHGEAIF
                     NMSASFHVLERGLEHQAATAPEIPPPEQLESDYEIRLRVAQRVPERVRPALTQKRAID
                     IRPVDPIDFVNPTPKPPRRQAWFRADGKLDEDLGLHQAIMAYASDFGLLGTGMTTHGL
                     SFADPNVQAASLDHLIWFHHPFRADDWLLYDMESPCAMNARAFNRGQIFQNGKLVASV
                     CQEGLMRVRK"
     gene            complement(3560..>4651)
                     /locus_tag="VFX11_06405"
     CDS             complement(3560..>4651)
                     /locus_tag="VFX11_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX4938288.1"
                     /translation="GRFEGDMPDVAPEIFKRKKNALTAAGSDWIPGFHDAVFSKRHHL
                     LPEIIRQLWLARDEAKKSRTQPLSQAIKIIMNSFYGVLGTPLCRFFDPRLASAITLRG
                     HDIMLQTRVLIEQQGYQVIYGDTDSVFVWLQGVRDDVGADGIGSQLAQFLNQWWRDKL
                     QEVFGLESCLELQYETHYRHFFMPTMRGSDTGSKKRYAGLIQSADTEKLVFKGLENVR
                     TDWTPLARRIQYDICWKIFHNEEVQGYLQNVVQDLHSGKLDEELVYRKRIRRRLAEYR
                     KNVPPHAQAAKKADQWLQQQGRTTRYERGGWVSYVMTVNGPEPLECRESRLDYDHYLQ
                     RQLAPAVDGILHFRNLSLQGIVQSQLSLF"
BASE COUNT          905 a         1359 c         1373 g         1014 t
ORIGIN      
        1 acgcgctgga actgtacaag ggtctgggtg cgaatggcgc gatcgtcaac ttcgccctga
       61 tccagctgac cgtgcaggtg atcattcgca agagcaaatt ccctgaaggc tacggccaga
      121 aatcccgtga attcgtgtgg agccagtacc cccgtttcat ggagtacatc aactcctcca
      181 ccaagagcat caagccgagc tactggctgg aggttccgga cgccgacaag ccgggttacc
      241 tggaaatgtt ccgtcaatcc cgtatcaagc tgcgcgacca gggcttctac gatggcaaaa
      301 tgctgagctt cctgagcaag gtgcgttgcc agaaggatgc atcgctggca gagtgcaccg
      361 ccaaagaccg tgaataatta cctccccggc acacaaaaag caggccacaa gcctgctttt
      421 ttatgagcgc agaaccaagt ctatgagcca atcaatagtc aaacgcaacc tgcgtgaatg
      481 ggtgtcctca ttcccggttt ttctggtcct gctgctggtc atcctgttca gcattgggga
      541 aaatttcaac gcgcaggtga accggctggg taattttctc tggcctgact accacctgtt
      601 gcgactcgat ctgccgacac cggactgcga tcccaatatc gatgtggaag cccgcgtgca
      661 ggaagtgttg aacaccgcaa cggatgatgc gttcggcgac atgttcggcg cgccggatgc
      721 ggacaccacg cgtcagtctg tacagaaaga tctggaactc tgcaggctga aacacagcga
      781 aatcgaacgc gtcaatgcgc agcgcaccga tggcattgcg attttcagca gcatcaacat
      841 gacgctggcg tcgctgtccc agctcagtcg cgacaacacc agtaacatgc tggtgctgat
      901 actgttcgtc tgcgccctga ccgccaccct cacctatcac cagatcggcc tgcgcccgat
      961 gcagaccgca ctggaccatc gcctgggcgc cgtagcccag ttcgttgcca acggtgcgct
     1021 gtcctattcc gcttaccatt acctgatcaa tggctgggcc tccggcacgg tgatccagaa
     1081 cgagccgatc cgctggtcct acatgatcgg gttcggcctg ctggcgctgg tagcgctggt
     1141 gcaggttttc aaactgccgg catcagtaaa acccggcggc tctttcggca aggcgctgct
     1201 ggcgattccg ctttattgct acatggcctt cggctccgcc aattactaca ttctgcagca
     1261 cgacaacccg cagggtatgg cgctgttcgt cgacaagatc atggacactt cgtcgatcta
     1321 tttgagcgtg ggcctgtaca tctgggtggg catgctggcc aagcagactt tcctgggcga
     1381 atacgtgttc cgtattttca aaccctggaa actgccgccg gaattgctgg cgttcgttgc
     1441 agtggtgatc atggcgattc ccacggcata caccggtgcg tccggcatca tcatcattgc
     1501 catgggtgca gtggtttaca ccgaattgcg ccgcgttggt gcgcgccgtc aactggcgct
     1561 cgccgccact gcgatgagcg gtagcctggg cgtggtgctg cgtccgtgcc tgctggtgct
     1621 gatcatcgcc gtgctcaaca aggaagtgac gacagatcaa atgtatggct ggggtgtgaa
     1681 gattttcatg ctgaccgcgc tgctgttctt cctggtatcg ctggtggcgc ggcaggggcc
     1741 gctcagggtg gcgccggtgg cagaggcgct gatgccaagc ctgcaggcgt tccgtccgct
     1801 ggttccctat gcgctgctgt ttaccctggt cgccgtcatc tatgcgttcg gactggattc
     1861 ctatctggat gaacattccg cgccttacat cctgccggtg atcatgctgg cgctactggc
     1921 gtacgagcgg ctgtttggca cgccggtgga acagcgcgac gattttcacg atagcgaacg
     1981 cgcatcgacg ctggaaggct ctgtccgcgc cgccaccacc aacaccaccg tgcacatcgg
     2041 cgcgttattg atgctgatgt gcctgggcct tacatcgggc ggtgtgattg aggattccgg
     2101 catcatccag gcggccaccg agcagggtag ccttttcagc aatatctggg tcgccatggc
     2161 ggcgctggtt ctgctgctgg tgttcatcgg catgatcatg gacccgttcg gtgtcatcat
     2221 cctggtgtcc ggcgcagtgg cgcaggcggc ctatgctcag ggcatcgatc cgctgcattt
     2281 ctggatggtc gtgctggtgg cgttcgagtt gggttatctg agcccgccag tggcggtgaa
     2341 ccacctgctg gcgcggcagg tggtgggcga ggatgaaatc gaaaaggcca tcgatgagtc
     2401 gaaagacggc agcttctgga tacgccatga acgctatacc ctgccgctga cggtgatgag
     2461 cgtatcgctg ttgatcgtgg cgtttgtccc gctggtatac aagaatttct tctagactgt
     2521 aaaggctgca acgcacatgc ctgaaagtag tagtgaggac atcatgagca atctgttaga
     2581 cgagctggtt cacctgctgg acctggaaac aatcgagcac aatctgtttc gcggccagag
     2641 ccgggatgtg ggcggccgca gtgtgttcgg tggccaggtg gtggcccagt ccctggttgc
     2701 ggccatgcgc actctcacgg aaaagcggca gtgccactcg ctgcatgcct atttcctgcg
     2761 tcccggtgat atcgaaaccc ccatcgtgta tgaggtggac cgcatccgcg acggtggcag
     2821 cttcacgacc cggcgcgtag tggccagtca gcatggtgag gcgatcttca acatgtcggc
     2881 ttcgttccat gtactggagc ggggccttga gcatcaagct gccacggcac cggagattcc
     2941 tccgccggag cagctggaat ccgattacga gatccgcctg cgggtggcgc agcgggtacc
     3001 agagcgggtg cgcccggcgc tgacccagaa acgcgccatt gatatccggc cggtggatcc
     3061 gatcgacttt gtgaatccca cgccaaaacc gccacgccgg caggcctggt tccgcgccga
     3121 tggcaagctg gatgaggatc ttggtctgca ccaggcaatc atggcctatg cgtcggattt
     3181 cggtctgctg ggcacaggca tgacgaccca tggtttgtcc ttcgctgatc ccaatgtgca
     3241 ggcggccagc ctggatcacc tgatctggtt ccatcatccg tttcgcgccg acgactggct
     3301 gttgtacgac atggaaagcc cttgcgcgat gaatgcgcgc gcgtttaatc gcgggcagat
     3361 tttccagaat gggaagctgg tggcgtcggt gtgtcaggaa gggttgatgc gggtgcggaa
     3421 gtgaggttgg aacacgaata aagcgcggtg ggatattaaa tctggacacg ccgtaaaccc
     3481 atccatgggg gctgctccgc ggcatccatg ccgcagagcg tccagatcta atatcccacc
     3541 acgctttatt cgtgttgccc tagaacagcg acagctgcga ctgcacaatc ccctgcagcg
     3601 aaagattgcg aaaatgcagg atgccgtcca ccgccggtgc cagttgccgt tgcaggtaat
     3661 gatcatagtc gagccgtgat tcgcggcatt ccagcggctc cggcccgttc actgtcatca
     3721 catagctgac ccagccacca cgttcatagc gagtggtacg cccctgctgc tgcagccact
     3781 gatccgcctt ttttgccgcc tgcgcgtgcg gtggcacgtt cttgcggtac tccgccagcc
     3841 ggcgccggat gcgcttgcga tacaccagct cttcatcgag tttgccgctg tgcaggtcct
     3901 gcaccacatt ttgcagatac ccctgcactt cctcgttgtg aaagatcttc cagcagatat
     3961 cgtactgaat ccggcgcgcc agtggcgtcc agtcggtgcg cacgttttcc aggcctttga
     4021 acaccagctt ttccgtatca gcggactgaa tcagcccggc gtaacgtttc ttgctgccgg
     4081 tgtcggagcc gcgcatggtg ggcatgaaaa aatgccggta gtgggtttcg tattgcagtt
     4141 cgaggcagga ttccagaccg aacacttcct gcagtttgtc gcgccaccac tggttgagaa
     4201 attgtgccag ctgcgaacca atcccgtcgg cgcctacatc atcgcgcacg ccctgcagcc
     4261 agacgaacac ggaatcggtg tcgccgtaaa tcacctggta accctgctgc tcgatcaaca
     4321 cccgcgtctg gagcatgatg tcgtggccgc gcaacgtaat cgcactggcc aagcgcggat
     4381 cgaagaagcg gcacaggggc gtgcccagca caccgtaaaa cgaattcatg atgatcttga
     4441 tggcttgcga cagcggctgg gtgcgggatt ttttcgcttc atcccgcgcc agccacaact
     4501 ggcggataat ctccggcagc aggtgatggc gcttgctgaa cactgcatcg tgaaaaccgg
     4561 gaatccagtc actgcccgca gcggtaagcg catttttctt gcgcttgaaa atctccggcg
     4621 ccacgtccgg catatcgcct tcgaaccggc c
//
LOCUS       DASUEG010000344         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_115523, whole genome shotgun sequence.
ACCESSION   DASUEG010000344 DASUEG010000000
VERSION     DASUEG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115523"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /gene="nuoC"
                     /locus_tag="VFX11_06410"
     CDS             <1..1445
                     /gene="nuoC"
                     /locus_tag="VFX11_06410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEX4938289.1"
                     /translation="GDLNIPSVREIWPNADWYEREVFDLFGIHFQGHPHLFRLLMPRY
                     WKGHPLRKDYPARATEFDPFILDIARQDEAQEALRFNPEEWGMKRSARDEDYMFLNLG
                     PNHPSAHGAFRIILQLDGEEIVDCVPDIGYHHRGAEKMAERQSWHSYIPYTDRIDYLG
                     GVMNNLPYVLAVEKLAGIEVPDRVKVIRVMMAEMFRITSHLLFLGTFIVDVGAITPIF
                     FAFTDRQKAYDVIEAITGFRMHPAWYRIGGVAQDLPNGWDKLVRTFVDWLPKRLDDYE
                     KAGLRNGILRSRTVGVAQYNTREALEWGVTGPGLRATGVNYDLRKARPYSGYENFDFD
                     VPVYHNGDAYDRCMVRINEMRQSLRIIRQCLDNMPAGPYKADHPLTCPPPKERTLQDI
                     ETLITHFLSVTWGPVIPAGESCQMIEATKGINSYYLFSDRSTMSYRTRIRTPSFAHLQ
                     QIPSVIRGSMVPDLIAYLASIDFVMADVDR"
     gene            1432..1956
                     /gene="nuoE"
                     /locus_tag="VFX11_06415"
     CDS             1432..1956
                     /gene="nuoE"
                     /locus_tag="VFX11_06415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX4938290.1"
                     /translation="MSTVNPTPSKLIVTDRGQVYEMAAEDRDEILHAIAHYDDSRAAS
                     IDALKIVQRRHGWVPDGAISVIGKLLNIPGSDVEGVATFYNRIYRQPVGRHVIAVCDS
                     ISCFLMGFDQIYGALQTKLGIKPGQTTTDGRFTLLPICCLGACDRGPVLMVNDNTHFN
                     ISVETLDQLLEQYQ"
     gene            1953..3281
                     /gene="nuoF"
                     /locus_tag="VFX11_06420"
                     /pseudo
     CDS             1953..3281
                     /gene="nuoF"
                     /locus_tag="VFX11_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261939.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
     gene            3285..>3582
                     /locus_tag="VFX11_06425"
     CDS             3285..>3582
                     /locus_tag="VFX11_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4938291.1"
                     /translation="MATIHVDGKQFEVDGTDNLLQACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYANADDKRGRLVMSCMTPSTDGTIISVTDDEAKAFRESVVEWLMVNH"
BASE COUNT          742 a         1134 c         1046 g          660 t
ORIGIN      
        1 aaggcgatct gaacattccg tccgtgcgcg agatctggcc caacgcagac tggtacgagc
       61 gcgaagtgtt tgatctgttt ggcattcact tccagggtca cccccacctg ttccgcctgc
      121 tgatgccgcg ttactggaag ggtcaccccc tgcgcaagga ctatccggcc cgcgccaccg
      181 aattcgatcc gttcattctg gacattgccc gtcaggatga agcccaggaa gccctgcgct
      241 tcaatccgga agaatggggc atgaagcgca gcgcccgcga cgaggactac atgttcctca
      301 acctgggccc caaccacccc agtgcccacg gtgccttccg catcatcctg cagctggacg
      361 gtgaagagat cgtcgactgc gtacccgata tcggctatca ccaccgtggc gccgagaaga
      421 tggccgagcg ccagtcctgg cacagctata ttccctacac cgaccggatc gactatctgg
      481 gcggcgtgat gaacaacctg ccctatgtgc tggcggtgga aaaactggcc ggcatcgaag
      541 tgcccgaccg ggtgaaagtc atccgggtaa tgatggcgga aatgttccgt atcaccagcc
      601 acctgctgtt cctgggtacc ttcatcgtgg acgtgggcgc gatcactccc attttcttcg
      661 ccttcaccga tcgccagaaa gcctatgacg tgatcgaggc catcaccggt ttccgcatgc
      721 atccggcctg gtatcggatt ggcggtgtag cgcaggatct gcccaacggc tgggacaagc
      781 tggtccgcac ctttgtcgac tggctgccca agcgtctgga cgactatgaa aaggccggcc
      841 tgagaaacgg catcctgcgc agccgcaccg tgggcgtggc ccagtacaac accagggaag
      901 cgctggaatg gggtgtgaca ggtccaggtc tgcgcgccac cggcgtcaat tacgacctgc
      961 gcaaggcccg cccgtattcc ggttacgaga acttcgactt cgacgtgccc gtctatcaca
     1021 acggcgatgc ctacgaccgc tgcatggtgc ggatcaatga aatgcgccag agcctgcgta
     1081 tcatccgtca gtgcctggac aacatgccgg ccggcccgta caaggccgat cacccgctga
     1141 cctgtccgcc gccgaaagag cgcaccctgc aggatatcga aaccctgatc acccacttcc
     1201 tcagcgtgac ctggggaccg gtgataccgg cgggtgaatc ctgccagatg atcgaggcga
     1261 ccaagggtat caacagctat tacctgttca gtgaccgcag caccatgagt taccgtacgc
     1321 ggattcgcac gccgagtttc gcgcacctgc aacagattcc gtcggtgatc cggggcagta
     1381 tggttcctga cctgatcgct tacctggcaa gcattgactt tgtgatggca gatgtcgacc
     1441 gttaatccca cacccagcaa actcatcgtc accgaccgcg gccaggttta cgaaatggcg
     1501 gcggaagacc gcgacgaaat cctccacgcc atcgcgcact acgacgattc gcgcgcggcc
     1561 agcatcgacg cgctgaaaat cgtgcagcgt cgtcacggct gggtgccgga tggtgccatt
     1621 tccgtgatcg gcaaactgct caatattccc ggctccgatg tggaaggcgt ggccactttc
     1681 tataaccgca tctaccgcca gccggtgggc cgtcatgtga tcgccgtgtg cgacagcatc
     1741 agctgcttcc tgatgggctt cgaccagatt tacggcgcac tgcaaaccaa actgggcatc
     1801 aagcccggcc agactaccac tgacggccgc ttcaccctgc tgccgatctg ctgcctgggc
     1861 gcctgcgacc gcggcccggt gctgatggtc aacgacaaca cccatttcaa catcagcgtc
     1921 gaaaccctcg accaactgct ggagcaatac caatgagctc caatgtgaat ctaggcctta
     1981 cccatatcgg tcctgaaacc cacccgctca cctggcgcta cagccaggcc ggcaaggcgg
     2041 tgctggacct gagaacctac gaatcgatgg aaggctacgc cgcgctgaaa aaagcggtgc
     2101 agatggcccc tgccgacgtg cagaaagtgg tgtaggattc caacctgcgc ggccgtggcg
     2161 gcgcgggttt ccccactggc gtgaaatggt cgctgattcc gatggggccc aacgccggcc
     2221 ccaaatatct ggtctgcaac gccgacgaaa tggagccggg caccttcaag gaccgcatgc
     2281 tgatggaaga actgccccat cagttggtgg aagccatggt cgtcgccggt tatgcgatcc
     2341 aggccacccg cgcctacatc ttcctgcgcg gtgaatacac cgtggcggcg gagcggctga
     2401 acaacgcgat tcatgaagca gaagccgccg gctatgtggg gaacaatatt ctgggctccg
     2461 gctggagcat ggatctgcac gtgcacaccg gtgccggccg ctatatctgt ggcgaggaaa
     2521 ccgcgctgat caactcgctg gaaggccgcc gtgccaaccc gcgtgccaag ccgccgttcc
     2581 cgcagatcgc cggtctgtgg ggcaagccca ccatcgtcaa taatgtggaa tccctcaaca
     2641 acgtgcctgc gatcctgctg cgcggcgccg agtggttcaa gggcctgtcg caaggcaaat
     2701 cgcccgacgc cggcaccaag ctgtacggta tttccggcaa ggccaaaaac accggtttgt
     2761 gggaactgcc catcggcacc acctgccgcg aactgctgta cacccacggc ggcggcatgc
     2821 aggacggcct ggaactgatg gcctggctgc cgggcggtgc gtccaccgac tttttgatgc
     2881 cggaacatct ggatctggca atggattacg acaccatcat gaaagccggc agccgcatgg
     2941 gcaccggcct gatcgcggtc atcgacaaca aacagaacat ggtgtcggtg gtgcgtaatc
     3001 tggagcagtt ttttgcgcgg gaatcctgcg gctggtgcac accctgtcgc gacggcctgc
     3061 cctggatcgt gaaagtgctg caggccattg aagatggcaa gggccagtcc ggcgacatcg
     3121 aattcctgca gcagatgacg cgcaatctcg cacccggcaa aacgttctgc gcccatgcac
     3181 ccggcgccgt cgagccgctg cagagcgcga tcaagtattt ccgtcctgct ttcgaacaag
     3241 gcattgcccc tgtggcaagc ccgcaaatta cgggtaagta acgcatggcc accatacatg
     3301 tagatggcaa acagtttgag gtcgacggca cagacaacct gctgcaggcg tgtctgtcgc
     3361 tgggtctcga catcccctat ttttgctggc atccggccct gggcagcgtt ggcgcctgcc
     3421 gtcagtgcgc agtgaagcag tacgccaacg ccgacgacaa acgcggccgt ctggtgatgt
     3481 cctgcatgac gccgtccacc gacggcacga tcatttcggt gaccgacgac gaggccaagg
     3541 ccttccgcga aagcgtggtg gagtggctga tggtgaacca cc
//
LOCUS       DASUEG010000345         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1376212, whole genome shotgun sequence.
ACCESSION   DASUEG010000345 DASUEG010000000
VERSION     DASUEG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376212"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2428
                     /gene="mfd"
                     /locus_tag="VFX11_06430"
     CDS             <1..>2428
                     /gene="mfd"
                     /locus_tag="VFX11_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX4938292.1"
                     /translation="PETQRSLEQVKQIRLLPAKEFPLNQSAISSFRDRFKAVFDVDHR
                     RCPVYQDISDGLATAGIEYYLPLFFENTATLFDYLPANTLVITPEQVQETVETFWTDC
                     TGRYEELRYDITRPLLPPQQLFLPNDEFHRRLKEFPRIHFRAHAAETDRAGHCNLPLS
                     EPPSLPINAKATNPLMHVEAYCMAAQAEQRRVLFVAESAGRKDTLQELLGRIRIHPKS
                     VESWQEFVDAKTPQPQILIAPLATGMVLTEPALSVITESQLFGHRVMQSRRRKRGDDH
                     AENVIRNLTELNAGDPVVHIDHGVGRYQGLQTLEIDGQQQEFLTLEYADKAKLYVPVS
                     SLHLISRYSGASDIMAPLHRLGTDQWAKEKRKAAEQIYDTAAELLSIYARRAAKPGHA
                     FKLSVLDYEAFCEGFPFEETPDQQAAIDAVIADMTSDRPTDRLVCGDVGFGKTEVAMR
                     AAFLAVQNGKQVAILVPTTLLAQQHYETFKDRFADWPVHIEVLSRFRTTKEQSRAIDQ
                     IAEGKIDIVVGTHKLLQDNLKFKDLGLLIVDEEHRFGVRHKEKLKELRADVDMLTLTA
                     TPIPRTLNMAMSGVRDMSIIATPPAKRLSIKTFVRESNNHVVKEAILRELLRGGQVYF
                     LHNEVKSIDRRAEEMQALVPEARIVVAHGQMRERELEQVMTDFYHKRFNVLMCTTIIE
                     TGIDVPSANTIIIDRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPNPKAITPDAVKRL
                     EAIQEAHDLGAGFTLASHDLEIRGAGELLGEEQSGNMHSIGFSLYMELLEEAVQSIKA
                     GKTPNLDKPF"
BASE COUNT          520 a          772 c          727 g          409 t
ORIGIN      
        1 atccggaaac ccagcgctcg ctggaacagg tgaagcagat ccgcttgctg ccggcgaaag
       61 aatttccgct caaccagagc gcgatcagtt ccttccgcga ccggttcaaa gcggtgttcg
      121 atgtggatca tcgccgctgc ccggtctacc aggatatttc cgacggcctg gccaccgccg
      181 gtattgaata ctatctgccg ctgttttttg aaaataccgc caccctgttc gactatctgc
      241 cggccaacac gctggtaatc acgccggaac aggtgcagga aaccgtggaa accttctgga
      301 cggactgcac tggccgttac gaggaactgc gttacgacat cacgcgcccg ctgttgccac
      361 cgcagcagct gtttctgccc aacgatgaat tccatcgccg gctgaaggaa tttccgcgca
      421 tccattttcg cgcccacgcg gccgaaacgg atcgcgccgg ccactgcaat ctgcccttga
      481 gtgaaccgcc gtcgctgccg atcaatgcca aggccaccaa tccgctgatg cacgtggaag
      541 cctattgcat ggcggcgcag gccgaacagc gccgcgtgct gttcgtggcg gaatcggccg
      601 gacgcaagga cacgctgcag gaactgctgg gccgcatccg cattcatcca aagtcggtgg
      661 aaagctggca ggaattcgtc gacgcaaaaa caccgcagcc gcagatcctg atcgcgccac
      721 tggccaccgg catggtgctg accgagccgg cgctcagcgt catcaccgag tcacagctgt
      781 tcggccaccg ggtgatgcag agccgccgtc gcaagcgtgg cgacgatcat gcggaaaacg
      841 tgatccgcaa cctgaccgaa ctgaacgccg gcgatccggt ggtgcatatc gatcacggtg
      901 tcggtcgcta ccagggtttg cagacgctgg agatcgacgg ccagcagcag gaattcctga
      961 cactggaata tgccgacaag gccaagctgt atgtaccggt gtcgtcgctg catctgatca
     1021 gccgctactc gggcgccagc gacatcatgg cgccgctgca tcgcctgggc acggatcagt
     1081 gggcgaagga aaaacgcaag gccgccgagc agatctacga caccgcggcg gaactgctga
     1141 gcatttacgc ccgccgcgcg gccaagccgg gccacgcatt caagctgtcg gtcctggatt
     1201 acgaagcctt ctgcgaaggc tttccatttg aggaaacgcc agatcagcag gctgccatcg
     1261 acgcggtgat tgccgacatg acgtcggacc gccccaccga tcgcctggtg tgcggcgacg
     1321 tgggtttcgg caaaaccgaa gtcgccatgc gcgcagcgtt tctggcggtg cagaacggca
     1381 agcaggtggc gattctggtg cccaccaccc tgctcgccca gcagcattac gaaaccttca
     1441 aggaccgctt cgccgactgg ccggtgcaca tcgaagtgct gtcgcgcttc cgtaccacca
     1501 aggagcagag ccgcgccatc gaccagattg ccgaaggcaa gatcgacatc gtggtcggca
     1561 cccacaagct gctgcaggac aacctgaaat tcaaggatct gggcctgctg atcgtcgatg
     1621 aggaacaccg tttcggcgta cgccacaagg aaaagctgaa ggaactgcgc gccgacgtgg
     1681 acatgctgac gctgaccgcc acgccgattc cgcgcacgct caacatggcc atgtccggcg
     1741 tgcgcgacat gtcgattatc gccacgccgc cggccaagcg gctgtcgatc aagaccttcg
     1801 tgcgcgagtc caacaatcat gtggtgaagg aagccatcct gcgggaacta ttgcggggcg
     1861 gtcaggtgta tttcctgcac aacgaagtga aatccatcga ccgccgtgcc gaggaaatgc
     1921 aggcgctggt gccagaggcc cgcatcgtcg tcgcccacgg ccagatgcgc gaacgcgagc
     1981 tggaacaggt gatgacggat ttctaccaca agcgcttcaa cgtgctgatg tgcaccacca
     2041 tcatcgaaac cggtatcgac gtgccgtcgg ccaacaccat catcatcgac cgcgccgaca
     2101 agttcggtct ggcgcagctg caccagttgc ggggtcgcgt cggccgctcg caccatcagg
     2161 cctatgccta cctgctcacc cccaatccga aggcgatcac gccggacgca gtgaagcggc
     2221 tggaagcaat ccaggaggcg catgatctgg gcgccggctt caccctggcc agccacgacc
     2281 tggaaatccg cggtgccggc gagctgctgg gcgaggaaca gagcggcaac atgcacagca
     2341 taggcttctc gctgtacatg gagctgctgg aggaagcggt gcagtcgatc aaggcgggca
     2401 agacgccgaa cctggacaag cccttcca
//
LOCUS       DASUEG010000346         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_606125, whole genome shotgun sequence.
ACCESSION   DASUEG010000346 DASUEG010000000
VERSION     DASUEG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606125"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFX11_06435"
     CDS             complement(<1..495)
                     /locus_tag="VFX11_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450283.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEX4938293.1"
                     /translation="MNLQGKKVTLHDMCLRDGMHPKRHQISIDQMIDVATALDDAGVP
                     LIEVTHGDGLGGASVNYGFPAASDEEYLDAVVSKLKKAKVSALLLPGIGTVDHLKMAA
                     DCGVGTIRVATHCTEADVSEQHIGLARKMGLDTVGFLMMAHMASPEKVLEQARLMESY
                     GANCI"
     gene            complement(517..1413)
                     /locus_tag="VFX11_06440"
     CDS             complement(517..1413)
                     /locus_tag="VFX11_06440"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEX4938294.1"
                     /translation="MTKKLRAAIIGPGNIGTDLLMKAKRSSWIEPVWMVGIEESEGIK
                     RARDFGMKVSTSGVDGLVPFVKEDNIQIAFDATSAYVHAENSRKLNALGVIMIDLTPA
                     AIGPFCVPPVNLTELLKEGRKENVNMVTCGGQATIPMVYAVSRIQPVEYGEIVATVAS
                     KSVGPGTRKNIDEFTRTTAGAIEKIGGAKKGKAIIIVNPAEPPLIMRDTVHCLTKDKP
                     DQKRITDSIHSMVADVQKYVPGYRLTNAPVFDGNRVSISLNVEGLGDFLPKYSGNLDI
                     MTAAALRTAEMYAEAMSTGAIK"
     gene            complement(1612..2406)
                     /locus_tag="VFX11_06445"
     CDS             complement(1612..2406)
                     /locus_tag="VFX11_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4938295.1"
                     /translation="MSLSLQQREQLGKELYDALRSGKTLRPLIERFPEIEIEDAYHIS
                     HAMLQHRLHNDGETVVGKKIGVTSKAVQDMLGVFQPDFGFLTSAMSVPNESSVKIAGN
                     LIQPRAEAEIAFLLKSDLQGPGVTETDVLNATASIMTCFEIVDSRIDNWKIKIQDTIA
                     DNASCGVYVLGNERVDPRGLDLPNLTVNVFKNGKPLSSGKGSAVQGNPLTAVAWLANT
                     LGEFGIPFKAGEVILSGSLVPLEPVVAGDEMHAEIDGLGSATVRFI"
     gene            complement(2403..>3217)
                     /locus_tag="VFX11_06450"
     CDS             complement(2403..>3217)
                     /locus_tag="VFX11_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938296.1"
                     /translation="HNKMRIHYSELGSAAASPVIFLQGSGPGASGWLNFRYNAQYFAD
                     QGFRVIIPDLPGFGDSDKPDLDYTLDYFVGVVTEFADLLDLGQFALVGNSLGGAVSLG
                     LALAQPQRVTHLILMGCGGLEDQITYFQKMPGIQAMTKVPLGSPDFTPAYLKQVLQLI
                     VHDPKHITDELITERFRILQTQNPAVFKRMVIPNITARLGEIQCPVLGFWGAQDRFCP
                     ISGAETLVTHCPDAQVITLSQCGHWVMIEHADLFNQRCVEFLRSKKAQGQAA"
BASE COUNT          551 a          888 c         1022 g          756 t
ORIGIN      
        1 gatgcagttg gcgccgtagc tttccatcag gcgggcctgc tccagcacct tctccgggct
       61 ggccatgtgc gccatcatca ggaagccgac ggtgtcgagg cccatcttgc gggcgaggcc
      121 gatgtgctgt tccgacacat ccgcttcggt gcagtgggtg gcgacgcgga tggtgccgac
      181 gccgcagtct gctgccattt tcaggtgatc gaccgtgccg atgcccggca acagcagtgc
      241 ggatactttc gcttttttca gtttcgatac cacggcgtcg agatattcct cgtcgctggc
      301 ggcaggaaaa ccgtaattca ccgatgcgcc gcccagcccg tcgccgtggg tgacttcgat
      361 cagtggcacg ccggcgtcat ccagcgcggt ggcgacatcg atcatctggt cgatggaaat
      421 ctggtggcgc ttggggtgca tcccgtcgcg cagacacatg tcgtgcagtg tgactttttt
      481 gccttgcagg ttcatgttca ttgcctccgg aatgggttac ttgatggcgc cagtggacat
      541 ggcttccgca tacatttcag cggtgcgcag tgcggcggcg gtcatgatgt cgaggttgcc
      601 ggaatatttg ggcaggaaat cccccaggcc ttccacgttg agtgaaatgg agacacggtt
      661 accatcgaat accggtgcgt tggtgagccg gtagccgggt acgtatttct gcacgtcggc
      721 caccatcgag tggatggaat cggtgatgcg tttctggtcc ggtttgtctt tggtgaggca
      781 gtgtacggtg tcgcgcatga tcagcggtgg ttcggccggg ttgacgatga tgatggcctt
      841 gccttttttc gcgccgccga ttttttcaat ggcgccggcg gtggtgcggg tgaattcgtc
      901 gatattcttg cgggtgccgg ggccgacgga tttggaggcg acggttgcga cgatttcacc
      961 gtattccacc ggttgtatgc ggctgactgc gtacaccatc gggatggtgg cctggccgcc
     1021 gcaggtgacc atgttgacgt tttccttgcg gccttctttc agcagttcgg tgaggttcac
     1081 cggtggtacg cagaaggggc cgatggcggc gggtgtgagg tcgatcatga tgacgcccag
     1141 tgcattcagc ttgcgcgagt tttcggcgtg tacgtaggcg gaggtggcgt cgaatgcgat
     1201 ctggatgttg tcttccttga cgaagggcac caggccgtcg acgccgctgg tggagacttt
     1261 catgccgaaa tcgcgggcgc gcttgatgcc ttcggattct tcgatgccca ccatccacac
     1321 tggttcgatc cagctgctgc gcttggcttt catcagcagg tcggtgccga tgttgccggg
     1381 gccgatgatg gcggcgcgta gtttcttggt catggttgtg tccttcttgc tttcaattga
     1441 attcggtttt acgagtgaat tctatggtgc gtggcaggac gcacggtgac agttgaatcc
     1501 ggacacgccg tgaacccatc cctgggggct tctctgcgac atccatgtcg cagagagtcc
     1561 ggcttcaact gtcaccgtgc atcctgcgtc gagcagtgtg ccattcgtct gttaaatgaa
     1621 tcgcactgtc gcgctgccga gcccatcgat ttccgcatgc atttcatcgc cggctaccac
     1681 cggttccagt ggcaccagcg agcctgacag aatcacttcg ccagccttga acggaatgcc
     1741 gaattccccc agcgtgttgg cgagccaggc gactgcggtg agcggattgc cctgcacggc
     1801 ggaacctttt ccgctgctca ggggcttgcc gttcttgaat acgttcaccg tcaggttcgg
     1861 cagatccagt ccgcgcggat cgacgcgttc gttgcccagc acatacacac cacaggacgc
     1921 gttgtcggca atggtgtcct gaatcttgat tttccagttg tcgatgcggg aatcgacgat
     1981 ttcaaagcag gtcatgatgc tggcggtggc attcagcaca tcggtctcgg tgacgccggg
     2041 gccctgcagg tcggacttca acagaaaggc aatttccgct tcggcgcggg gctggatgag
     2101 gttgcccgcg attttgacgc tggattcgtt cggcactgac atggcggagg tgaggaaacc
     2161 aaaatccggc tggaatacgc ccagcatgtc ctgcacggct ttcgaggtga cgccgatttt
     2221 tttgcccacg acggtctcac cgtcgttgtg caggcgatgc tgcagcatgg cgtgggagat
     2281 gtggtaggcg tcctcgattt caatttccgg gaagcgttcg atgagcgggc gcaatgtttt
     2341 tccgctgcgc agggcgtcgt acagttcctt gcccagttgt tcccgttgct gcaggctcag
     2401 gctcatgcgg cttgtccctg tgcttttttg ctgcgcagaa attccacgca gcgctggttg
     2461 aacaggtcag cgtgttcgat catgacccaa tggccgcact ggctcagggt gatgacctgc
     2521 gcatccgggc aatgggtcac cagcgtctcg gcaccgctga tggggcagaa gcggtcctgc
     2581 gcgccccaga agccgagcac ggggcactgg atctcgccca gccgcgcggt gatgttggga
     2641 atcaccatgc gcttgaacac ggcgggattc tgggtttgca gaatgcggaa gcgttcggtg
     2701 atcagttcat cggtgatgtg tttgggatcg tgcacgatca gttgcagtac ctgtttcaga
     2761 tacgcggggg tgaaatccgg tgatcccagt ggcactttgg tcatggcctg gatgccgggc
     2821 attttctgga aataggtgat ctggtcttcc agtccgccac agcccatcag aatcaggtgg
     2881 gtcacccgtt gcggttgtgc cagcgccagg cccagactca ccgcgccgcc cagtgaattg
     2941 cccaccagcg caaattgccc caggtcgagc aggtcggcga attcagtcac cacacccacg
     3001 aaatagtcca gcgtgtaatc cagatccggt ttgtcgctgt cgccgaatcc gggcaggtcg
     3061 ggaatgatca cgcgaaaacc ctgatcggca aaatactgcg cgttgtagcg gaagttcagc
     3121 cagccgctgg cgccgggacc gctgccctgc aggaaaatca ccggagacgc cgccgctgaa
     3181 cccagttcgc tgtaatgaat gcgcatcttg ttgtgcg
//
LOCUS       DASUEG010000347         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_663974, whole genome shotgun sequence.
ACCESSION   DASUEG010000347 DASUEG010000000
VERSION     DASUEG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663974"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFX11_06455"
     CDS             <1..1037
                     /locus_tag="VFX11_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX4938297.1"
                     /translation="EVNNHNSLDTTKLRTSASISYDNLWQKHHSISLQTQNSPEEPDE
                     SRVLAGTYIFPVVDDVSRVVFYAVSSESDVSTLTDLTVVGSGEIYGTRLVRALDSSPG
                     YVHSLSLGFDLKDFDESVNLVGADTDSTPIKYSLFTAMYNGSFLRDDSTTRVGFSVTN
                     GLRDFLGGGDAEEFQNKRYYSHPNFFYVQGNFKHQQRFENDWRLNGRSKIQIADSALI
                     SNEQLSAGGAASVRGYYESQDTGDHGVVASADVETPDILDTKSSELRMRAFVDGAWLQ
                     MKDALSTDDQGDNRRVETEDTLLSAGLGTDFSMGKYLFFTLDLGVALREAVDIEEGDL
                     RSHASLVLEF"
     gene            1182..>3435
                     /locus_tag="VFX11_06460"
     CDS             1182..>3435
                     /locus_tag="VFX11_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX4938298.1"
                     /translation="MKERPDVINGLRQTGVYSLISLLAWQPILVRADVAPTELPKANV
                     DWVSQGAATRVETATDMTVKQISDRVSLQWDTFNIGDKASVKFDQPNSSSIALNRVQG
                     GSGSRINGKLSANGIIYIVDSNGVIFGRNSIVEVASLVATALNVDEDLIMNESFVKAI
                     NDGKAAFEGGAGENAAIVLEGSIDGMEGARIITGEGGSVFLFAPRVETQNGSSITTPG
                     GQTILAAAKDQVYLAASQDPDLRGLLVEVNEGGDIDHAGKIVADRGNITLAALNIDQR
                     GQLKATTSVNANGTIRLLARDTVNSIGALDDSTLQNQLLAGVLMADGEVDSDMKAFDA
                     KYAKSSRAGRVTLHEGSVTEVVPESSSKTSADSNVQPVSFVEVMGKTIEVKDRASITS
                     RGGVVSLVATEKPDDPMAVGTPENDASVVLHSGSRIDVSGMDTAVVAMERNSLEIELR
                     GDELKDSPMQRNDADIRGETAFVDIRDTDEIEFADISAYLAKIERTVDERLSAGGKVN
                     VYSEGNVDFQQGAQVDVSGGTVHYKSGYVLESQLVDNGNLVYISDADPNVNYDAVISG
                     NERYNARWNVVEKIKSSSVGAGKGTFVKGYVEGKDAGSFNIKSRAADYGGTVTADTVS
                     GPYQRTGSNQVSGGKIDLDLSHFDFSQQAIALVAESEYQQYLRDKLAGTADAGTTTIS
                     VERFAGSADSLSIKTRNDLSLAEGTALTLDGNGALDLKGGDVSIQGDITNRAGAVAIA
                     AAEFQSSNTTS"
BASE COUNT          764 a          881 c         1083 g          707 t
ORIGIN      
        1 tggaagtgaa caaccacaat tccctggaca ccacgaaatt acgcacatcc gccagcatca
       61 gctacgacaa tctgtggcag aagcatcaca gcatttcgct gcagacgcag aattcgccgg
      121 aggaaccgga tgaatcccgc gtactggcgg gcacctacat ttttccggtg gtggacgatg
      181 tcagccgggt cgtgttttat gcggtgagct ctgaaagcga cgtgtcgact cttacggatc
      241 ttaccgtggt cggtagcggt gaaatttacg gaacgcggct ggtgcgcgcc ctcgattcca
      301 gccctggtta tgtgcattcg ctgtcgctgg gctttgatct gaaggacttc gatgagagcg
      361 tgaatctggt gggtgccgat acggattcca cgcccatcaa atacagcctt ttcactgcca
      421 tgtataacgg cagtttcctg cgtgatgatt ccactacccg cgtgggtttc agcgtgacca
      481 atggcctgcg cgattttctg gggggcggtg acgccgagga gttccagaac aagcgctatt
      541 acagccatcc caacttcttt tacgtgcaag gcaatttcaa acaccagcaa cgcttcgaaa
      601 atgactggcg cctgaacggc cgcagcaaga tccagattgc ggattcagcc ttgatcagca
      661 atgagcaact gagtgcaggc ggcgcggcca gtgtgcgtgg ctactacgaa tcgcaggaca
      721 ccggcgatca cggcgtggtg gcatcggcgg atgtggaaac accggacatt ctggacacca
      781 aatcctctga gttgagaatg cgtgccttcg tcgatggcgc ctggctgcag atgaaggatg
      841 cgctgtcgac cgatgatcag ggcgacaacc gccgggttga aaccgaggac acactgttga
      901 gtgcggggtt gggcacggat ttcagcatgg gcaaatatct gttttttaca ctggatctgg
      961 gggttgccct gagggaagcc gtggatatcg aagaaggtga tctgcggtcg cacgcgtccc
     1021 tggtgctgga gttctgagtc gcggctttta cgactttatt gtgactttgt tacgactgga
     1081 ttttgcggtt gggccgcccg gccggttaca tcaaaccgtc atatatcgtt aacaggatcg
     1141 tgtgagaatc gcgcgactca agcaggtacc cgggggagct ggtgaaagaa cgtcctgatg
     1201 tgatcaatgg tttgcggcag acaggtgtat acagtctgat ttcgctgctg gcgtggcaac
     1261 ccattctggt gcgagccgat gttgctccaa cggaactgcc gaaggccaat gtggactggg
     1321 tcagccaggg tgccgccacg cgggtggaaa cggccaccga catgacggtg aagcagattt
     1381 ccgaccgcgt ttctttgcag tgggacacgt tcaatatcgg tgacaaagcg tcggtcaagt
     1441 tcgatcagcc caacagcagt tccattgcat tgaatcgggt gcagggtggt tcgggttcgc
     1501 ggatcaatgg caaactgtcg gccaatggca tcatctatat agtcgatagc aacggtgtga
     1561 tctttggcag gaattccatc gtggaagtgg cgtcgctggt tgcgacggcg ctgaatgtcg
     1621 atgaagacct gatcatgaac gaatccttcg tcaaggccat caacgacggc aaggccgcgt
     1681 tcgaaggcgg cgctggcgag aatgcggcga tcgtgctgga aggcagcatc gatggtatgg
     1741 aaggtgcgcg catcattacc ggcgaaggcg gcagtgtatt tctgtttgcg ccccgggtgg
     1801 aaacgcagaa cggatcgtcg atcaccacgc ccggcggcca gaccattctg gcggcagcaa
     1861 aggaccaggt gtatctggcg gcatcgcagg atcccgatct gcgcggcttg ctggtggagg
     1921 tgaatgaggg tggtgacatc gatcacgccg gcaagattgt ggcggaccgg ggcaacatca
     1981 cgctggccgc actgaatatc gaccaacgcg gccagttgaa ggccaccacc agcgtgaatg
     2041 cgaacggtac catccggctg ctggcgcggg acacggtcaa ctctatcggt gccctggacg
     2101 atagtactct gcagaatcag ttgctggccg gtgtgctgat ggcagacggc gaagtggatt
     2161 ccgacatgaa ggcgttcgat gccaagtacg cgaaatcctc cagggccggc cgggtgacac
     2221 tgcacgaagg cagtgtgacg gaagtggtgc cggaaagcag ttcgaaaacg tctgccgaca
     2281 gcaatgtgca accggtgtcg tttgttgaag tgatgggcaa gaccattgag gtgaaagacc
     2341 gcgccagcat taccagtcgt ggcggtgtgg tctcgctggt ggctacagag aagcccgatg
     2401 atcccatggc ggtgggaacc cccgaaaacg acgccagtgt cgttctgcat tcgggcagca
     2461 ggatcgatgt ttccggcatg gacaccgcag tggtggccat ggaacgcaat tcgctggaaa
     2521 ttgaattgcg tggcgacgag ctgaaagatt cgccgatgca gcgcaacgat gccgacattc
     2581 ggggcgagac cgcctttgtc gatattcgcg ataccgatga aatagaattc gccgacattt
     2641 cggcctacct ggccaagata gagcgcacgg tggatgagcg cctgagcgcc ggtggcaagg
     2701 tcaacgttta cagcgaaggc aacgttgatt tccagcaggg cgcgcaggtg gatgtgtccg
     2761 gcggcaccgt tcattacaag tccggctatg tactggaaag ccagctggtg gataacggca
     2821 atctggtcta catcagcgat gccgatccca atgtcaatta tgacgccgtt atcagcggca
     2881 atgagcgtta caacgcccgc tggaacgtgg tggagaaaat caaatcctcc agcgtaggtg
     2941 caggcaaggg aacctttgtc aaaggctatg ttgaaggcaa ggacgccggt tcctttaaca
     3001 tcaagtcccg ggccgcagat tacggtggca cggtgactgc cgataccgtc agcggaccgt
     3061 atcagcgcac cggcagcaat caggtgtcag gcggcaagat cgatctggac ctgagtcatt
     3121 ttgacttcag ccagcaagct attgcgctgg tggcagaaag tgagtaccag cagtatctgc
     3181 gggacaagct ggcgggcacg gccgatgccg gcactaccac aatttcggtg gagcggtttg
     3241 ccggttcggc cgacagtctt tccatcaaaa cccgcaatga tctgtcgctg gcagagggta
     3301 cggctctgac gctggatggc aacggcgcgc tcgacctcaa gggaggcgat gtcagcattc
     3361 agggagacat taccaatcgg gctggcgctg ttgcgattgc ggcagcggaa ttccagagct
     3421 ccaacacgac cagcg
//
LOCUS       DASUEG010000348         8958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1308832, whole genome shotgun sequence.
ACCESSION   DASUEG010000348 DASUEG010000000
VERSION     DASUEG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308832"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1427)
                     /gene="fliD"
                     /locus_tag="VFX11_06465"
     CDS             complement(15..1427)
                     /gene="fliD"
                     /locus_tag="VFX11_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX4938299.1"
                     /translation="MATITAAGTGSGIDVENIITKLTEAERTPTEQRLLLRETNIQAE
                     ISAFGSLKSSLSDFRKSLTNLSSLQSLAARSATSSKPESFTVTADNTASVGSSQIEIV
                     NLASAHKMVSTANFAGPTTAVGAGTMTISLGSASFNVQIVGGQNNTLAGIRDAINNAT
                     GNTGITASILTVSNGMGGTQSKLVLSADDTGTANTISVAVTGDSDGNDTDNVGLSAFL
                     NANMEQKSTAANALIRVDGFDVTSSTNVFKNAIQGVTLTAVKANPTVNETLTVGINKT
                     AIKDNLALFVENFNALSETLKFLTKYDPETQEAGLLTGDSTVRSIETQLRRTITNVVE
                     GLDSNFSSLASLGITTQRDGTLKLDSTKLDSALTTNFDDVANLLGGEDGIAKKLDSTL
                     NNFLKTGGLISTRNETFLKQLKDIDSQRERLELRMTSYEERLRAQYAAMDAIVANLNS
                     TGDYVAQQMESISQITAKKK"
     gene            complement(1597..2046)
                     /locus_tag="VFX11_06470"
     CDS             complement(1597..2046)
                     /locus_tag="VFX11_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEX4938300.1"
                     /translation="MINNSTTGLEAKSSYTSVEKQKPAAAAPSAKSETVTVTAPVEKQ
                     KPAAEQVPVQEQQTEPADLSREQREEVTRKVAEAIPKVRELMQRNQRSLDFEVSEQEN
                     RVIITVIDKENDKVIRQIPPEDVLQIAEAIDQGLEQNLGGMIFSSRV"
     gene            complement(2151..3695)
                     /locus_tag="VFX11_06475"
     CDS             complement(2151..3695)
                     /locus_tag="VFX11_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4938301.1"
                     /translation="MPQIINTNISSLNAQRNLNKSQGALQTSLQRLSSGLRINSAKDD
                     AAGLAISNRFTTQVRGLNVATRNANDGISFAQTTESALDEITTSLQRIRDLAVQSAND
                     TNSASDRKSLQSEVDQLVSEINRIAETTTFNGSKVANGSLKSLNFQIGANAGETVAVK
                     GVDARASSLGSQPGAVQTTFDGGNAAAASQTALTIQVGSSANATSVDVMSTANGGTIT
                     SATAAQLTDTEDDNYGTGFAKSLAERINSLREDGVEGLEGVYASATTTFDYSDVAGTV
                     AAEAYVGAGTLANGDLVINGVDIGPVEVQEKDANGALVTAINAKSDITGVKASLDDNS
                     RLVLTAADGRDVVVTTANGSEEILFDGGDTNAGDITALASQVKTGELTISAQDTINIT
                     ETGSLANTDTTDDNVQAVGTIANADITTAAAANITMKAVDSALGQIDSFRADLGAIQN
                     RFESTIRNLSAVSESLSAANSRIKDADFAAETAELSKNQVLQQAGISVLAQANALPQQ
                     ALSLLG"
     gene            complement(4146..5696)
                     /locus_tag="VFX11_06480"
     CDS             complement(4146..5696)
                     /locus_tag="VFX11_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4938302.1"
                     /translation="MPQIINTNISSINAQRNLNKSQGALQTSLQRLSSGLRINSAKDD
                     AAGLAISNRFTTQVRGLNVAVRNANDGISLAQTTESALDEITTALQRIRDLAVQSAND
                     SNSSSDRLSLQAEVDQLLDEVDRISTTTTFNGRNVLDGSLKSYYFQIGANANEGVEVS
                     GVDARVSSLGSQPGAVQTTFDGGNAAAASQTSLAIQIGTSANATSVDVMSAPNGGTIT
                     SATAAELIDTESDNYGSGFAKSLAERINNLREDGVEGLEGVYASATTTFDYSDVAGTA
                     AADAFVGAGSLAHTDLVINGVNIGPVEVQERDADGALRQAINSKTEITGVRANLDDNG
                     RLVLSADDGRDMVVTTSTTASDILYDGGDTANATDIAAMTNQVKTGELTISAQDTINV
                     TTTGSLANTDTTDDNVQAVGTVANADISTIEGCNQTMKSIDSALAQIDGFRGELGAVQ
                     NRFESTIRNLSAVSESLAAANSRIRDADFAAETADLSKNQVLQQAGISVLAQANALPQ
                     QVLSLLQG"
     gene            complement(5842..7065)
                     /gene="flgL"
                     /locus_tag="VFX11_06485"
     CDS             complement(5842..7065)
                     /gene="flgL"
                     /locus_tag="VFX11_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX4938303.1"
                     /translation="MSIRISTSQTFTQGTNAILDNQSKVSLTQLQLSKGTRILKPSDD
                     PIGSSVALNLQRQIDNTTQYIANGQAAQARLAVEDSVMDNVSTILGRVRELALLGANG
                     TLNHTDREAICIEIEQRYDELLGLANTQLSSGEYLFSGFQSNVQPFSKNGTGSVVYNG
                     DQGVQMINVNSTVQVESGNSGDAVFMNIPTGNGSFVTGADDNNTGSGVIGSAVMDDPT
                     AYVGDVYTIDFSLDGGGNLVYQVTGANTGPVYAAPLPSFDEDGVVTFNGISYTISGLP
                     APGDSFTVEPSYAQDVFTSVKQIVDALKKSTDTDALQATFRNALGVGMKNLDQAITHM
                     DEVRAGVGSRLNVIESETNINESLQVEAKSALSLIVDLDYASAVTELNKQTLALQAAQ
                     QSFVKIQGLSLFEFI"
     gene            complement(7078..>8958)
                     /gene="flgK"
                     /locus_tag="VFX11_06490"
     CDS             complement(7078..>8958)
                     /gene="flgK"
                     /locus_tag="VFX11_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX4938304.1"
                     /translation="YSRQRTELATRIPLQYGSGFIGQGVDVEGITRISNQFVTDQLRR
                     DTQNFNSYNSYYEYAVRMDSLLGDESTAITPTLQSFFDGLEELANDPSSIAARQVLLS
                     EGRALVNRFNTVYDQVYQQNETLNTEMATIASQVTQLAKSIANLNDSIRTVASNNVGQ
                     LPNDLLDQRDEAIRQLSELVGVQVVADDNLTVNVFIGSGQPLVLGGDSYTLSTDTGNS
                     GISRNNVILTAGNNNQDITAQLSGGRLGGLLQVRQELIDPIFNELGRMATVLADTFND
                     QHQLGMDLNNQLGGLFFGDINSPVAEAQRVSASLDNAGAASLSVTISDAGALTADNYE
                     LRFDGANYSLINAASNSTIATFADPGAGGTVVVASEGFTLNFIAGASVAGDSFMITPT
                     RMGAAEISLEIDNVKQVAAAMPVRTQLPQSNTGAGFVENVVVSDTTAADFATPYTLTP
                     PYTVVFTSANTYDVINTTTSAVVVGGVAFTPNQSNGLLQQAGLYPASGYDVVYNGVPA
                     LGDQIVIQYNNGGVGDNRNALLLGKLSSTKTIDNGATTYQSAYGQLVSGVGTRTRDAE
                     VGQEASESIMRQSEAQRESISGVNLDEEAADLMRFQQAYQASARVIQVSSELFDSILN
                     SL"
BASE COUNT         1796 a         2447 c         2545 g         2170 t
ORIGIN      
        1 ctgctgaaat taggctattt cttcttggct gtaatttgcg aaatcgactc catctgctgc
       61 gcaacgtaat cacctgtcga gttcaggttg gctacgatcg catccatggc agcatactgg
      121 gcacgcaaac gttcttcata cgacgtcatc cgcaactcaa gtctttcccg ctgggaatcg
      181 atatccttga gctgtttcag gaacgtttcg ttacgggtgg aaatcaggcc accggtcttg
      241 agaaaattgt tcagggtgga atccagcttc ttggcgatac cgtcttcgcc acccagcagg
      301 tttgccacgt catcaaaatt ggtggtgagc gcgctgtcga gcttggttga gtccagtttc
      361 aatgtgccat cacgctgggt ggtgatgccc agggaagcca gggagctgaa attggaatcc
      421 agaccctcaa ccacattggt gatggtccgg cgcagctggg tttcgatgga gcgcacggtg
      481 gaatcgccag tcaacaaacc tgcttcctgg gtttccggat catatttggt caggaatttg
      541 agggtttcac tcaatgcatt gaagttctcg acgaacaatg caaggttgtc tttgatggcg
      601 gttttgttga tacctaccgt cagtgtctcg ttaacagtgg gattggcttt caccgccgtc
      661 agggtcacac cctgaatcgc gttcttgaaa acattggtcg acgatgtcac gtcgaacccg
      721 tcaacacgga tcagcgcatt ggcggcagtg cttttctgct ccatgttggc attcaggaaa
      781 gccgacagcc cgacgttatc cgtatcgttg ccgtcggaat cgccggtgac ggccaccgag
      841 atggtattgg cagttccggt gtcgtctgcc gacagcacca gcttggattg ggtaccgccc
      901 atgccgttgg ataccgtcag gatactggca gtgatgccgg tgttgccggt tgcgttattg
      961 atggcatccc gaatgccggc cagcgtattg ttctggccgc ccacgatctg gacattgaac
     1021 gatgcgctgc ccagggaaat ggtcatggtg ccggcgccga ctgcggtggt aggtccggca
     1081 aagttggcgg tggacaccat tttgtgtgcg gatgccagat tgacgatttc gatctggctg
     1141 ctgcctaccg acgcagtgtt gtcggcagtc acggtaaagg attcaggctt gctggaagtt
     1201 gccgaccggg cggcaagact ttgcagggag gacaggttgg tcagggattt tctgaaatca
     1261 gagagggagc ttttgagcga gccaaacgct gaaatttcag cctggatatt ggtttcgcgc
     1321 agcaacaggc gctgctcggt gggagtgcgc tcagcttcgg tcagcttggt aatgatgttt
     1381 tcgacatcaa tacctgatcc ggtaccggct gcggtaatgg ttgccatttt aacctctctg
     1441 cagattcctc tgtgcctgtc agattactga cagcaccagg taatctagca attaacagac
     1501 caatctattc gtcgtaacat cactccccat tgatctggtt atcggcacct gacaggcaat
     1561 ctttagggcc cgcctttttt ccggcgcaca aatagctcaa acgcgcgaac tgaaaatcat
     1621 gccacccaga ttctgctcca acccctgatc gatcgcctcg gcaatctgca acacgtcttc
     1681 cggcggaatc tgtcggatga ccttgtcgtt ttccttgtcg atcacggtga tgatgacgcg
     1741 gttttcctgc tccgacacct cgaaatccag actacgctgg tttctctgca tcagctcgcg
     1801 aaccttggga atggcctcag ccactttgcg ggtgacttct tcgcgctgtt ccctggagag
     1861 atccgccggt tcagtctgct gctcctgcac gggaacctgt tccgccgccg gtttctgttt
     1921 ctccaccggg gccgttaccg taacagtttc agacttggcg gaaggggcag cagcagccgg
     1981 cttctgcttt tcaacagaag tatagctgct cttcgcctcc aggccggtcg tactgttatt
     2041 gatcatgact gccacctgct atcgcaatgg aaacgcgagc acccggaacc tttcgatccc
     2101 ggggctcgct ctttcttttt tgcctaactt ttcagttaag ggacaacacc ttagccgagc
     2161 agcgagaggg cctgctgcgg cagtgcgttg gcctgagcca gcaccgagat acctgcctgt
     2221 tgcaatacct ggttcttgga cagttcagct gtttcagcag cgaaatctgc gtccttgatc
     2281 cgggaatttg cagccgacag actttccgat acagccgaca ggttgcggat ggtcgattca
     2341 aagcggttct gaatcgcacc cagatcagcg cggaaggagt cgatctggcc cagcgcactg
     2401 tctactgcct tcatggtgat gttggcagca gctgctgtgg taatgtcggc attggcgatg
     2461 gtgcctacag cctgaacatt gtcgtcggtg gtatcagtgt tggccagcga accggtttca
     2521 gtgatgttga tggtgtcctg agctgaaatg gtcagttcac ctgtcttcac ctggctggcc
     2581 agcgcagtaa tgtctcccgc gttggtatcg ccaccgtcga acagaatttc ttctgaaccg
     2641 tttgcagtgg taaccaccac gtcacgaccg tcagcggcag tcagcaccag gcgactgttg
     2701 tcatccaggc tggccttgac accggtgata tccgatttgg cgttgatggc agtgaccagt
     2761 gcgccgttag cgtctttttc ctgcacttca accggaccga tatccacacc gttgatgacc
     2821 aggtcgccgt tggccagtgt gcctgcgcca acatacgctt cagcagccac ggtaccggca
     2881 acatcactgt aatcaaaagt ggttgtggcg ctggcgtaaa caccttccag accttccaca
     2941 ccatcttcac gcaggctgtt aatgcgctcg gccagggact tggcaaagcc ggtgccatag
     3001 ttgtcgtctt ctgtatcagt caactgggca gcggtagccg aggtaatggt gccaccgttg
     3061 gctgtcgaca tcacatcaac cgatgtggcg ttggcggaag aaccgacctg gattgtcagg
     3121 gcagtctggc tggctgcagc agcgttgcca ccgtcaaagg tagtctgcac tgcacccggc
     3181 tgtgaaccca gggaggaagc gcgagcgtcc acgcccttga ctgctacggt ttcacccgcg
     3241 ttggcgccga tctggaaatt cagggatttc agcgagccgt tggcaacctt ggagccgttg
     3301 aacgtagtgg tttcggcaat gcggttgatt tcagacacca gctggtcaac ttccgactgc
     3361 agcgacttgc ggtcagatgc gctgttggta tcgttggctg actgtacggc cagatcgcga
     3421 atacgctgca gcgaagtggt gatttcgtcc agggcgcttt cagtggtctg cgcgaaggag
     3481 ataccgtcgt tggcgttgcg ggtagcaacg ttcagaccgc gaacctgagt ggtgaaacgg
     3541 ttggagatgg ccagaccggc agcgtcgtct ttcgcgctgt tgatgcgcag accggaagac
     3601 agacgttgca gggatgtttg cagtgcgccc tgtgacttgt tcaggttacg ctgcgcgttc
     3661 agtgaggata tgttggtatt gataatctgg ggcatggtgt tctcctgcta tttaaacacg
     3721 acctcaattg agttgaggtt aatctgtttg gctgtgcctc tgttgcgttt gaggccgtgt
     3781 gaactacttc atctgcctac acccagttta tcggcgtcgt ttttttgaac tttaaggaga
     3841 atcctcaaat ttcttcaggc tgcgtggtgc tccccctcat ccacgtcaac tctttgtaat
     3901 tgcggtggtt tttatcccgt gccccgactg cttccgaagc ccgttccatt aaatcgtttt
     3961 ggacttaaaa cctgcccaca aataacggcc ctgcagaagc gccggaattc cggcactgtc
     4021 gccccacctg cgtgaaattc gatggatggc gacagaaatt agacgacggc cgacctggtt
     4081 tcaaaagagc aaaaaaaaag aggaaccgaa gttcctcttt tctatcggct gtcggccgcg
     4141 cttcattagc cctgcaagag gctgagcacc tgctgcggca aggcattggc ctgtgccagc
     4201 accgagatac cggcctgctg cagcacctga ttcttcgaca ggtccgccgt ttcggccgcg
     4261 aagtccgcat cacggatacg cgagttggca gccgccagac tttccgatac cgccgacagg
     4321 tttcgaatgg tcgattcaaa gcggttctgc accgcaccca gttcaccccg gaagccatcg
     4381 atctgtgcca gcgcgctgtc tatcgatttc atggtctggt tgcagccttc gatggtggag
     4441 atgtcggcat tggctaccgt acccaccgct tgcacgttgt catcggtagt atcggtattg
     4501 gccaggcttc ctgtggtggt gacgttgatg gtgtcctgtg ccgagatggt cagttcaccg
     4561 gtcttgacct ggttggtcat tgcggcgata tcggttgcgt tggctgtatc accgccgtcg
     4621 tacagaatgt cggacgccgt tgtggaagtg gtgacaacca tatcgcggcc atcgtccgca
     4681 gacagcacca ggcgaccatt gtcgtccaga ttggcccgca caccggttat ttcagtcttg
     4741 gagttgatgg cctgacgcaa ggcaccatct gcgtcacgtt cctgcacttc gacagggcca
     4801 atattgacgc cattgatcac cagatccgtg tgcgcaaggc taccggcgcc aacgaaggca
     4861 tcggcggcgg ctgttccggc aacgtcactg taatcgaacg ttgtggtggc actggcataa
     4921 acaccttcca gaccttccac cccgtcttca cgcagattgt tgatgcgttc cgccagcgat
     4981 ttggcaaagc ccgaaccata gttgtcgctt tctgtgtcga tcagttcggc tgcggttgca
     5041 gaagtgatgg tgccaccgtt gggcgccgac atgacatcca ccgacgtggc gttggccgag
     5101 gttccgatct ggatcgccag actggtctgg ctggcagcgg cagcgttgcc accatcgaag
     5161 gtagtctgaa ccgcgcccgg ctgggaaccc agcgaactga cccgcgcatc gacgccgctc
     5221 acttccacgc cttcattggc gttggcgccg atctggaaat agtaggactt cagcgatcca
     5281 tccagcacat tgcggccgtt gaaggtggtg gtggtggaaa tgcggtccac ctcatccagc
     5341 agctggtcca cttccgcctg caacgacaac cggtccgatg agctgttgga atcgttggcg
     5401 gactgtaccg ccagatcgcg aatacgctgc aaagcggtgg tgatttcgtc cagggcgctt
     5461 tctgtggtct gcgccaggga gataccgtcg ttggcgttgc gcactgccac gttgaggcca
     5521 cgtacctggg tggtgaaccg gttggaaatg gccagaccgg cagcgtcgtc tttcgcgctg
     5581 ttgatgcgca gacccgacga caggcgttgc agtgaggttt gcagggcgcc ctgtgacttg
     5641 ttcaggttgc gctgtgcgtt gatggacgaa atgttggtat taatgatctg aggcatgttg
     5701 ttttctccag cgtcgtcccg cgttttttat acgggaactc agtaaataaa ttcgtttatg
     5761 cgtttacggg cctttcgggg atttgtatca atctatcctc ctcgccactg gtcacgtttt
     5821 cgtcgtttaa gtctgtacat atcaaataaa ctcaaacagt gagaggccct ggatcttgac
     5881 aaagctttgc tgcgcagcct gcaatgcaag ggtctgcttg ttcagctctg ttaccgccga
     5941 ggcataatcg agatccacga tcagggacag cgcggatttg gcttccacct gcaggctctc
     6001 gttgatattg gtttcgcttt caatcacgtt cagccggcta cccacccctg cgcgcacttc
     6061 atccatatgg gtgatggcct gatccagatt tttcattccc acaccaaggg cattgcgaaa
     6121 ggttgcctgc agcgcatcgg tgtctgtgga ttttttcaag gcatccacaa tctgttttac
     6181 cgaagtgaac acatcctgcg cgtaactggg ctccacggtg aaactgtcac ccggagctgg
     6241 cagaccggag atggtgtagc tgatgccatt gaacgtgacg acgccgtcct catcgaaact
     6301 gggcagaggc gcggcgtaaa ccggtccggt attggcgccg gtgacctggt aaaccagatt
     6361 gccgccacca tccagggaaa agtcgatggt atagacatca ccgacatagg cggttggatc
     6421 atccatgacg gcagaaccaa tgacgcctga gcctgtgttg ttgtcgtcgg ctccggtgac
     6481 aaacgatccg ttgccggtgg ggatattcat gaacaccgca tcgccactgt tgccactttc
     6541 cacctggacc gtgctgttga cgttgatcat ctgcacgccc tggtcgccgt tatagaccac
     6601 gctgcccgtg ccattcttgc tgaatggctg cacgttggac tgaaagccgg aaaacaggta
     6661 ttcgcccgag gaaagctgcg tattcgcaag gcccagcaat tcgtcatagc gctgttcgat
     6721 ctcgatgcag atggcttcgc gatcggtatg attcaacgtg ccattcgcgc cgagcagtgc
     6781 cagttcgcga acacggccaa gaatggtgga cacgttgtcc atcacggaat cttccaccgc
     6841 cagcctcgcc tgcgcagcct gaccgttggc aatgtactga gtggtattgt cgatctggcg
     6901 ttgcagattc agggccaccg aagagccgat cggatcatcg gatggtttca ggatccgagt
     6961 gccttttgac agctgcaact gggtcagact gactttggac tggttgtcca gaatggcgtt
     7021 ggtgccctgg gtaaatgtct gtgaagtcga gattctaata ctcatcgcac atcagcctca
     7081 caacgaattc agaatggagt caaacagttc ggacgatacc tgtatgacac gggctgatgc
     7141 ctgataggct tgctgaaagc gcatcaaatc ggcggcttct tcgtcgagat tgacgccaga
     7201 gatggattca cgctgcgctt cgctctggcg catgatgctt tctgacgctt cctgccccac
     7261 ttccgcatcg cgggtgcggg ttcccacgcc actgaccagc tgaccgtagg cgctttgata
     7321 cgtggtggca ccattgtcga tcgttttggt tgagctgagt tttcccagca gcaaagcgtt
     7381 gcggttgtcg cccacgccac cattgttgta ttgaatgacg atctggtcgc ccagcgccgg
     7441 cacgccgttg tacaccacgt cataacccga cgcgggataa agccctgcct gttgcagcaa
     7501 accgttcgac tggttcggtg taaaggccac accaccgacg acgactgcac tggtggtggt
     7561 gttgatgacg tcgtaagtgt tggcgctggt aaataccacg gtgtagggcg gtgtcagcgt
     7621 atagggtgtg gcgaaatccg ctgcggtggt gtcactcacc accacgtttt cgacaaagcc
     7681 ggcacccgtg ttggattgcg gcagttgcgt gcgcacgggc attgccgctg ccacctgttt
     7741 cacgttgtcg atttcaagac tgatttccgc cgcgcccatc cgggtggggg taatcatgaa
     7801 cgaatctccg gcaacggaag caccggcgat gaaattcagg gtgaaacctt ccgacgcgac
     7861 caccactgtt ccgcccgcgc ctggatctgc gaaggtagcg atggtggaat tgctggccgc
     7921 attgatcagc gaataattcg cgccatcgaa acgaagctca tagttgtcgg cggtcaacgc
     7981 gccggcatca ctgatggtta ccgacagaga agcggcgccg gcattgtcca ggctggcgga
     8041 cacgcgctgc gcttccgcca cgggggaatt gatatcgcca aagaacaggc caccaagctg
     8101 gttattcaga tccatcccca gctgatgctg gtcattgaag gtgtcagcca gaacggtcgc
     8161 catgcggccc agttcattga atatgggatc aatcagttcc tgccgcacct gcagcaggcc
     8221 accgagcctg ccacccgaga gttgcgcggt gatgtcctga ttattgttgc ccgctgtcag
     8281 gatgacgttg ttgcggctga ttccggaatt gccagtgtca gtggacagcg tgtaggagtc
     8341 cccacccagc accaatggct gaccactgcc gataaaaacg ttgaccgtca aattgtcgtc
     8401 cgccaccacc tgaacgccca ccagttcgga cagttgacgg atggcttcat cgcgctgatc
     8461 cagcaaatca ttgggcaact ggccgacatt gttggatgca acggtgcgaa tggaatcgtt
     8521 aagattggcg atgctttttg ccagctgggt gacctgcgag gcaatggtcg ccatttcggt
     8581 attcagcgtt tcattctgct ggtagacctg gtcatacacc gtattgaatc ggttaaccag
     8641 ggcccgccct tcactcagca atacctgacg cgcggcgatg gacgagggat cgttggccaa
     8701 ctcctccaga ccgtcaaaaa aggattgcag cgtcggtgta atggcagtgg attcatcgcc
     8761 cagcaacgaa tccatgcgta ctgcgtattc gtaataggag ttataggaat tgaagttctg
     8821 ggtatcgcgc cgcagctgat cggtcacaaa ctggttgctg atgcgggtga taccttcgac
     8881 atccacgccc tgaccgataa agccgctgcc atattgcagt ggaatgcgtg tggccagctc
     8941 ggtacgctgc cggctgta
//
LOCUS       DASUEG010000349         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_398922, whole genome shotgun sequence.
ACCESSION   DASUEG010000349 DASUEG010000000
VERSION     DASUEG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398922"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFX11_06495"
     CDS             <1..982
                     /locus_tag="VFX11_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938305.1"
                     /translation="QSALQQLQQKFDRYFAEREKSDPPPPPLPTYLYLPIAFESDTPA
                     RLRLTALNLQYHLLRNRFDALDVKHTLKFAGGTVTAQSLTLGLPANSQITRAQFQVTN
                     NLKPGFALNGIAGSASLNGHTGIDLTEGSTVSHRVDNATPLQLQHLLLALLPLTDTVQ
                     ATLQILRDNQDSPAGAVLAQTQLQLNQTGVRQWAVASIAPLLLDSGPCWLRLTVKQGR
                     LFWLGQNAPGHVQFTTGAGPGKAVRTLQLMAMLSAAGANENGMAGLSVQVNNVGASSS
                     GNTEEGIQIDLAAGLNGHFGSGTAAPAPVAFVSGSAGNITVSNLQVEYNI"
     gene            complement(1019..1657)
                     /locus_tag="VFX11_06500"
     CDS             complement(1019..1657)
                     /locus_tag="VFX11_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4938306.1"
                     /translation="MDLYFSYISTYSQKVLLGLYEKNVAFTPHLVDLTSQEERARFRE
                     FYPFGKVPLLVRDDGRMIPESSIILEYLDQTFPQSPRLIPADQEDARQVRLLDRMCDL
                     YLNDPVVSLILESWKPEAQQDSEVMDKAAERIGLMYRFLGDHLSGRDFLAGNQFTMAD
                     CAAIPPLYFAKAFADFSSVGQLQGYWERVSQRPAVVRMWREAEPFVRALMGE"
     gene            complement(1805..2701)
                     /locus_tag="VFX11_06505"
     CDS             complement(1805..2701)
                     /locus_tag="VFX11_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4938307.1"
                     /translation="MSSIDVTQPDSPTGSVPIQQQWPAPLSAREWDRLFPGVMDGVGV
                     MGGAANVIMQLARLPVGHGVADSKVESGALFKHPIKRARTTFTYLAVAMMGTTEEKLA
                     YRKAVNKSHAQVRSAEGDKVQYNAFDPELQLWVAACLYWGFMDTYGKLHGEPDRARLA
                     EFYRLAEPLGTTLQVRPGMWPADLDAFERYWQAGLDKLQIDDKVRIFLTRLVDLKFLH
                     PLISRPVGPFHRFLTTGFLPPSVREQMRLDWDAGKQQSFERFLGIVGRVNALMPRIVR
                     QFPFNLVMWDFRRRLRKGLPLV"
     gene            2924..3454
                     /locus_tag="VFX11_06510"
     CDS             2924..3454
                     /locus_tag="VFX11_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX4938308.1"
                     /translation="MDLSATLNIDPYDWPSIGVSLLCGLIIGLERQLRGKPVGIRTSA
                     LIVLGTYVFIASSNHVSNEVSDPSRIIGQVITGIGFLGAGVMLARDGMVIGVTSAATI
                     WALAAVGVCIAVGNIFSAIKLSFVVVAVLVGVDLLEDYSQALTRGVHMRYRNWRTRNG
                     PGNGGSNGNAPQDHPR"
     gene            complement(3466..4290)
                     /gene="cysQ"
                     /locus_tag="VFX11_06515"
     CDS             complement(3466..4290)
                     /gene="cysQ"
                     /locus_tag="VFX11_06515"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX4938309.1"
                     /translation="MSLALNRELLQQVLDIAVAAGDAIRTIYEHPDKVAVITKSDDSP
                     VTAADHAAHAIIVERLTQLTPAIPVLSEESDMPTFEVRRQWSRYWLVDPLDGTKEFIE
                     GSGEFTVNIALIEQHQPVLGVVTVPVKDMAYVAAKGTGAFRIDQGQWQPIRVRPVPDK
                     RLAIVGSRRHGAEKLQPVLTRIENSGFQVEMANMGSSLKFCLIAEGKADCYPRLGPTS
                     EWDTAAAQAVLLEAGGCVVDTKFVPLQYNRKESLLNPEFLALGDPAFDWPRYLQIG"
     gene            complement(4287..>4541)
                     /locus_tag="VFX11_06520"
     CDS             complement(4287..>4541)
                     /locus_tag="VFX11_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011218294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4938310.1"
                     /translation="FTVYMLIVGVIDNVLKPILLGRGVKVPMLVIFVGAIGGFLAAGI
                     IGLFVGAVFLVLGYQLGVEWLKTTERRALEAAREEREKSA"
BASE COUNT          926 a         1518 c         1286 g          811 t
ORIGIN      
        1 gcagtccgca ctgcaacaat tgcagcaaaa attcgaccgc tatttcgccg aacgggaaaa
       61 atccgaccca ccgccaccac cgctgccgac gtatctatac ctgccgattg ccttcgaaag
      121 cgacaccccc gcacgcctgc gcctcactgc gctgaacctg cagtatcacc tgctgcgcaa
      181 tcgcttcgat gcactcgatg tcaaacacac gctgaaattt gccggcggca cggttaccgc
      241 ccagtcgctg acactcggcc tgccggccaa cagccagatc acccgcgccc aattccaggt
      301 caccaacaat ctgaagcccg gcttcgcgct gaacggcatc gcgggcagcg ccagcctgaa
      361 cggccacacc ggcatcgacc tgacagaagg cagcaccgta tcacaccgcg tcgacaacgc
      421 cacaccgctg cagctgcaac acctgctgct ggcactgctg ccgctcaccg acaccgtgca
      481 ggccacgcta caaattctgc gcgacaacca ggattccccc gccggcgccg tactggcaca
      541 aacccaattg caactgaatc agacaggtgt gcgccagtgg gccgtcgcca gcatcgcgcc
      601 actgctgctc gacagcgggc cctgctggct gcgtctcacc gtgaagcagg gccgtttatt
      661 ctggctgggg caaaacgcgc cgggccacgt gcagttcacc actggcgccg gcccaggcaa
      721 agccgtgcgc acgctgcaac tgatggcaat gttgagtgcg gcaggagcaa atgaaaatgg
      781 aatggcggga ctgtccgtac aggtgaacaa cgtcggcgcc agcagcagcg gcaacacgga
      841 ggaaggaatt cagatcgatc tggcggcagg cctcaacggg catttcggca gtggtaccgc
      901 tgcgccggca ccggtggcat tcgtgtccgg cagcgcgggc aatatcacgg tcagcaacct
      961 gcaggtggaa tacaacattt agcgggtgtc actccgtttc cattcaccca tccggccttc
     1021 attcgcccat cagcgcgcgc acgaaaggtt ccgcctcgcg ccacatgcgc accaccgccg
     1081 gccgctgcga cacccgttcc caataaccct gcaactgccc caccgaggaa aaatcagcaa
     1141 acgccttggc gaaatacagc ggtgggatcg cagcgcagtc cgccatggtg aactggttgc
     1201 ccgccagaaa atcccgcccg ctcagatggt cgcccagaaa gcggtacatc aggccgatcc
     1261 gttccgccgc cttgtccatt acctcgctgt cctgctgcgc ttccggtttc cagctttcca
     1321 gaatcaggct gactaccgga tcattcaggt acagatcgca catgcggtcc agcaggcgca
     1381 cctggcgcgc atcttcctga tccgccggaa tcagccgcgg gctttggggg aacgtttggt
     1441 caaggtattc cagaatgatg ctggattcag gaatcatgcg gccgtcgtcg cgcaccagca
     1501 ggggcacctt gccgaacgga tagaattcac gaaagcgcgc ccgctcctcc tggctggtga
     1561 gatccaccag gtggggtgtg aaggcaacgt ttttttcgta caaccccagc aggacttttt
     1621 gggaataggt cgaaatgtag gaaaaataca gatccatcgc acgggactcc ttcgttcttg
     1681 ttatcggtga ggcaactacc gccagacgtt ggataccaga ccatcagacg ctggctacca
     1741 gaatagtccc gatcataccc gaacgcgaat cccgcgccgc ggcgcgcctg gcgcccagcg
     1801 gctgtcacac cagcggcaac cccttgcgca ggcgacgacg gaaatcccac atcaccagat
     1861 tgaacggaaa ctggcgcacg atgcgcggca tcagcgcgtt gacacggccc acgatgccca
     1921 ggaagcgctc gaaggactgt tgcttgcccg catcccaatc caggcgcatc tgctcgcgca
     1981 ccgatggcgg caggaagccg gtggtcagaa aacggtggaa ggggcccacc ggcctgctga
     2041 tcagcggatg caggaatttc agatccacca gacgggtcag gaagatccgc accttgtcat
     2101 cgatctgcag cttgtccagg ccggcctgcc agtaacgctc gaacgcatcc agatccgccg
     2161 gccacatgcc cgggcgcacc tgcaaggtgg tgcccagcgg ttcagccaga cggtagaact
     2221 ccgccagccg ggcccggtcc ggttcgccgt gcagcttgcc gtaggtgtcc atgaagcccc
     2281 agtacaggca ggctgctacc catagctgca attcgggatc aaacgcgttg tactgcacct
     2341 tgtcgccctc ggcagaacgc acctgtgcat gagacttgtt aaccgccttg cggtaggcca
     2401 gcttttcctc ggtagtcccc atcatggcca ccgccagata ggtaaacgtg gtgcgggcac
     2461 gcttgatcgg atgcttgaac agggcgccgc tttccacctt gctgtccgcc acgccatgcc
     2521 cgacaggcag ccgcgccagc tgcatgatca cgttggcggc accgcccatc acccccaccc
     2581 cgtccatgac ccccgggaaa agccggtccc attcgcgggc gctcagcggc gccggccact
     2641 gctgctggat cgggacggag ccagtggggc tatcgggctg ggtaacatca atggacgaca
     2701 ttcggaaact ccgcctgtac ctggttgata ggaggggtgc ttgagacata tgaatatcat
     2761 tgtttcaatg cgtgaacatt gatcagaatg atcaaattat tggtcgttac tgataaccgc
     2821 gccagccggg tggaccggca ccctgcagga agccacggca aatcctgccg accgggcaaa
     2881 gcctgcttat actgagaaaa taacagaaca gcggagccgc actgtggatt taagcgcaac
     2941 cctgaatatc gacccctatg actggccctc tatcggggta tccctgctgt gcggcctgat
     3001 cattggcctg gaacggcaac tgcggggcaa gcccgtgggc atccgcacct cagcactgat
     3061 cgtgctgggc acctatgtgt tcattgcctc gtccaaccac gtcagcaacg aagtatccga
     3121 tccctcgcgc atcatcggcc aggtcatcac cggcattggt tttctagggg ccggcgtcat
     3181 gctggcccgg gatggtatgg tgatcggtgt aacctctgcc gccaccatct gggccctggc
     3241 cgcggtaggg gtgtgcatcg cggtgggtaa tatcttcagt gcgatcaagc tgtcattcgt
     3301 ggtggtggcg gtgctggtgg gggtggacct gctggaagac tattcccagg ccctgacgcg
     3361 gggcgtacac atgcgctacc gcaactggcg cacgcgcaat ggtcccggca acggcggcag
     3421 caacggcaat gcgccccagg accatccgcg ctgagggcgg gggccttacc cgatctgcag
     3481 ataacggggc cagtcgaacg ccgggtcccc cagcgccaga aactccggat tcagcaggga
     3541 ttccttgcgg ttgtactgca acggcacgaa tttcgtgtcc accacgcagc cacccgcctc
     3601 cagcagcacg gcctgcgccg ccgccgtatc ccactctgac gtaggcccca gccgcggata
     3661 gcaatccgcc ttgccctcgg cgatcaggca gaatttcagc gagctgccca tgttggccat
     3721 ctccacctgg aatccgctgt tttcaatccg cgtcagcacc ggctgcagtt tctccgcacc
     3781 gtgacgccga cttccaacga tggccagccg cttgtccggc accggccgca cccggatcgg
     3841 ctgccactga ccctgatcga tccggaacgc accggtgcct ttggccgcca cataggccat
     3901 atccttcacc ggcaccgtca ccacgcccag caccggctga tgctgttcga tcagggcaat
     3961 gttcacggtg aattcgccgc tgccttcgat gaattccttg gtgccatcca gcgggtccac
     4021 cagccagtaa cggctccact ggcgccgcac ctcgaaggtc ggcatgtcgg attcttccga
     4081 cagcacggga atcgccggcg tcagttgcgt caggcgctcg acaatgatgg cgtgggccgc
     4141 atggtcggcg gcggtgactg gcgaatcgtc ggacttggtg atcaccgcca ccttgtccgg
     4201 atgttcataa atggtgcgga tggcatcgcc ggctgccacc gcaatgtcca gcacctgttg
     4261 cagcaattcg cggttcaacg ccaggctcat gccgatttct cccgttcctc gcgcgccgct
     4321 tccagcgctc tgcgctcggt ggtcttcagc cactccacgc ccaattgata ccccagcacc
     4381 aggaacacgg cccccacaaa caggccgatg atgcccgctg ccaggaaacc gccaatggcg
     4441 cccacgaaaa tcaccagcat gggcactttc acaccgcgcc ccaacagaat cggtttcagc
     4501 acgttgtcga tcacgccgac gatcagcatg tacacggtaa a
//
LOCUS       DASUEG010000350         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1308644, whole genome shotgun sequence.
ACCESSION   DASUEG010000350 DASUEG010000000
VERSION     DASUEG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308644"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VFX11_06525"
     CDS             complement(<1..1341)
                     /locus_tag="VFX11_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4938311.1"
                     /translation="MGKIAWCWKLLLCLWLALWGTGRADVAGASAPLTARSGLVLEVG
                     VGAQVLQLAPFIEYARTGMEPRAPEALVNAAPDWQQNRTASINLGYQKEPVWFRTVWQ
                     TDPAAGHDFMLEFTSAQLDELTVFLYEGGRLLRRWQTGDRLPFADRPFPDRNFVFPVT
                     LQPGQEYQLYFRVHNSEAMEFPVLLGERTAYSDFSNRRAMIDGVFYGILLIMAAYSFA
                     LYLILLDRTYLFYVSYVLSMLAFFLWQQGVLYQSFFPQWPQVQHYGAALISLLVFTSI
                     AMFFGQFLELGRTLPRSWLVYKMLLVLHGIYCVLLVWLDYQTVMYLMVSSTVLSTIVA
                     ASAIIRMALRGSRSAQIVLSGWALFLFCLMFFTAAKTGLIYNEFMATYGLRLGISFEI
                     LIFSFALSFRINQARKEKEVALQQATLERVERMRAQDLALQKEMELRQAKEAALQ"
     gene            complement(1527..>2859)
                     /locus_tag="VFX11_06530"
     CDS             complement(1527..>2859)
                     /locus_tag="VFX11_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX4938312.1"
                     /translation="ARWSLRCNPSSGNLHPTEAYLVSRGIAGLTDGVHHYRPDVHGLE
                     LRCAFTSAQQNIEPLLLLGLSSVHWREAWKYGERAFRYCQHDVGHALAGVSYAAAVLG
                     WRVQPLFEVGDDALAHLLGLDRTGEFVAEEGEHPDLLLAILPDKRATDHLDGLALLQS
                     LQPSLEQGDWAGQANVLDRRHFYCWPVIDAVTQASHKPDLDVPMDTQLPQPLPLPIPS
                     LCTEPAARLFRQRRSAQAFDGVTGIEATDFFRLLDHLLPRPGIAPWDTLPWAPRVHPV
                     LFVHRVQGLAPGLYALPRSEQGQTLLRTRLRQEFSWSAVAAAPDHLPLFQLIVARTER
                     TAMRLSCHQDIAGASAFSLGMLAEFDSHIQDTPWRYRELFWEAGVLGQVLYLEAEACG
                     LRGTGIGCYFDDAVHEVLGITDHSLQSLYHFTIGGPVMDARLVSLPPYARR"
BASE COUNT          616 a          925 c          870 g          448 t
ORIGIN      
        1 ctgcagcgcc gcctccttcg cctggcgcaa ttccatttcc ttctgcagag ccagatcctg
       61 cgcccgcatg cgctcgacgc gctccagcgt tgcctgctgc agcgccactt ccttttcctt
      121 gcgcgcctga ttgatgcgga acgaaagcgc gaatgaaaaa atcaggatct cgaaacttat
      181 ccccaaccgc aggccatagg tcgccatgaa ttcgttgtag atgaggcctg tcttggcagc
      241 ggtgaaaaac atcaggcaga acagaaacag cgcccaacct gacagcacga tctgcgcgga
      301 gcgcgatccc cgcaaggcca tccggatgat cgccgacgcc gcgacaatgg tggacagcac
      361 ggtgcttgac accatcaggt acatcaccgt ctggtaatcc agccagacca gcaacacgca
      421 gtaaatgccg tgcagcacca gcagcatttt gtacaccagc cagctgcggg gcaacgtgcg
      481 ccccagttcc agaaactggc caaaaaacat cgcaatggaa gtaaaaacca gcagcgagat
      541 aagtgctgcc ccatagtgct gcacctgcgg ccactgggga aaaaacgatt gatacagtac
      601 gccctgctgc cacaggaaaa aggcgagcat cgacagcaca taggacacgt aaaacagata
      661 ggtgcggtcc agcaggatca ggtacagcgc gaaggaatac gcggccatga tcagcagaat
      721 accgtagaac acaccgtcga tcatggcgcg acggttactg aaatcactgt aggcggttcg
      781 ctcccccagc agcaccggaa actccatggc ctcgctgttg tgcacgcgga aataaagctg
      841 gtattcctgg ccgggctgca acgtgacggg aaagacaaaa ttgcggtcgg ggaacgggcg
      901 gtcggcaaat ggcagtcgat cccctgtctg ccagcgccgc agcaaccggc caccctcgta
      961 cagaaagaca gtcagctcat ccagctgcgc gctggtgaat tccagcatga aatcgtgccc
     1021 agcggccggg tccgtctgcc agacagtgcg gaaccatacc ggctccttct ggtaacccag
     1081 gttgatcgac gcagtgcggt tctgctgcca gtcaggtgcg gcgttcacca gcgcttccgg
     1141 cgcgcggggc tccatgccgg tccgggcata ttcgatgaac ggcgccagct gcagcacctg
     1201 cgcgcccacc ccgacttcga gcaccaggcc cgaacgggcg gtcaggggtg cgcttgcgcc
     1261 ggccacatcg gcacggccgg tcccccacag ggccagccac aggcagagca gcagcttcca
     1321 gcaccaggca atctttccca tcgtttccac atcggggttg ggcggagtat ggcgagcacc
     1381 aagccggagc cgcgcggcag gcacactgag acaaaacaga ctatttgttc aggatagagg
     1441 attcccgtga atcgtccaac cggagagagg caccgaacag catcgcaaat cggctgactt
     1501 aaggggctga gcggcgccgg acgggttcag cgccgcgcgt agggcggcag gctcaccagc
     1561 cgcgcatcca ttaccggccc gccgatcgtg aagtggtaaa ggctctgcag cgagtgatcg
     1621 gtgatcccca gcacctcatg caccgcgtca tcgaaatagc agccgatgcc cgtgccgcgc
     1681 aggccgcagg cttccgcttc cagatacagc acctgcccca gcactcccgc ctcccagaac
     1741 agttcgcggt aacgccaggg cgtgtcctga atgtgggaat cgaattccgc cagcatgccc
     1801 aggctgaagg cactggcacc ggcgatgtcc tgatggcagc tcagccgcat ggcggtgcgc
     1861 tcggttctgg cgacaatcag ctggaacagc ggcagatggt cgggtgccgc tgccacggcc
     1921 gaccagctga attcctggcg caagcgggtt cgcagcaagg tctgaccctg ttcgctgcgg
     1981 ggcaaggcgt acagacccgg cgccagaccc tgcacgcggt gcacgaacaa aaccgggtgt
     2041 acgcgcggcg cccagggcag cgtgtcccag ggcgcgatgc cgggccgggg cagcaggtga
     2101 tccagcaggc ggaaaaaatc cgtcgcctcg atgccagtga cgccatcaaa cgcctgcgcc
     2161 gatcggcgtt gacggaaaag gcgggccgcc ggttcggtgc agagcgacgg gatcggcagc
     2221 ggtaagggct gcgggagctg cgtatccatc ggcacgtcca gatccggctt gtgactggcc
     2281 tgcgtcactg catcgatcac cggccagcag tagaagtggc ggcggtcgag cacattggct
     2341 tgcccggccc agtcgccctg ctccagactg ggttgcagac tctgcagcag cgccagcccg
     2401 tccagatgat ccgtcgcgcg cttgtcaggc aggatcgcca gcagcagatc cggatgctcc
     2461 ccttcttccg ccacaaactc gcccgtgcga tccagcccga gcagatgcgc cagcgcgtca
     2521 tcgcccacct cgaacaacgg ctgcacacgc cagcccagga cagcggcggc ataactgaca
     2581 ccggccagtg catgtcccac atcgtgctgg caatagcgga acgcgcgctc gccatatttc
     2641 caggcttcgc gccagtgcac ggaactcagg cccagcagca acaggggttc aatgttttgc
     2701 tgcgcggatg taaaggcaca gcgcaattcc aggccatgca catccgggcg gtagtgatgc
     2761 actccatccg tcagtccggc aatgccccgg ctgaccagat acgcttcggt ggggtgcagg
     2821 ttgccgctgg agggattgca gcgcagcgac cagcgcgcc
//
LOCUS       DASUEG010000351         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_575914, whole genome shotgun sequence.
ACCESSION   DASUEG010000351 DASUEG010000000
VERSION     DASUEG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575914"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFX11_06535"
     CDS             <1..890
                     /locus_tag="VFX11_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX4938313.1"
                     /translation="RGSICHNDVVAFYEMLETVGRNERIHLFIKSDGGNGKASIRIIN
                     LIRQYTDQLVALVPLECASAATMLAIGANEIRMGPMAFLSAVDTSLTHDLSPIDRDNN
                     RVSVSLDELNRIVRLWDSQKSASERENAYKHLFNHVHPLVIGAVDRAESLSIMLCEEI
                     LSYHMKDSAKIKEIAVTLNSKYPSHAYPILFGESRRIGLHVKMLDKEVNTLLLELNEL
                     YSEMGQRATTDFDEIRSHSNEILNILETSGKQIFYQNDKEWFYRTEERRWVTLNDNSN
                     WHRLEKKNGKTVRSTLHIS"
     gene            912..1586
                     /locus_tag="VFX11_06540"
     CDS             912..1586
                     /locus_tag="VFX11_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX4938314.1"
                     /translation="MIIVHHLENSRSQRVLWLLEELGLQYEVKRYNRDSKTMLAPPEL
                     RAVHPLGKSPVITDGDRVIAESGAIIEYLVRKYDPAHQFHPVAGSDDELDFSYWLHYA
                     EGSLMPLLVMKLIFNHIQRAPMPFFVKPVAKGISGKTLRSFIQPNLDTHLEFVENRMR
                     GRQWFVGDSFSAADVQMTFPLEAAAARDNLATKYPSLQAFLQRVHERPAYRRALERGG
                     PYALMS"
     gene            1652..2923
                     /locus_tag="VFX11_06545"
     CDS             1652..2923
                     /locus_tag="VFX11_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX4938315.1"
                     /translation="MLVAPCAHDEEDRLRALARYAVLDTSPEADFDAVTQLLSFICDT
                     PITLVSLVDRDRQWFKSRQGLDATETSRDLAFCAHAILQPDPLIVEDVTLDPRFADNP
                     LVLSAPNIRFYAGAPLITHDGFALGTLCAIDIKPRTFSSTQLHALTTLARHVVAILEL
                     RLKLRETEDLNRELARAQRELEENIAYRSRFFATVNHEMRTPLNAIAGFAQRLQKRIV
                     VETVPDYVHEGLDLIGAAARRLVDLVSDVLDMSKIEVGKMTLNEQPFSPAALLREVCD
                     TLQVVAEEKGVLLQLQIDPALPAQYVGDAKKLGQIALNLVSNAVKFTPPQKHVRVLCE
                     PVENGMRLVVQDEGIGIAEQNRKKVFLPFEQFHTTTELSEKGTGLGLAIVKNLVDLMH
                     GTVTLQSEFGKGTTFTLILPFSAVAISSASE"
     gene            2956..>3248
                     /locus_tag="VFX11_06550"
     CDS             2956..>3248
                     /locus_tag="VFX11_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938316.1"
                     /translation="MAPWKFSFFIRSYTETQGCDEKGFNMRKFFFSALMLAVWNFSLP
                     SAFAFNGFDNMCGWIRTAPGKARIVYMRHDGEWRTYRLYVPQSYQPNAPLVVD"
BASE COUNT          728 a          885 c          910 g          725 t
ORIGIN      
        1 cccgcggcag catctgccac aacgatgtcg tggcgtttta tgaaatgctg gaaacggtgg
       61 ggcgcaacga acggattcac ctgttcatca aaagcgatgg cggtaatggc aaggcctcga
      121 tccgcatcat caacctgatt cgccagtaca ccgatcagct ggtggcgctg gtgccgctgg
      181 agtgtgcgtc cgccgccacc atgctggcga ttggcgccaa tgaaatacgg atgggcccga
      241 tggcgtttct gagcgcggtg gatacctcgc tgacccatga tctgtcaccc atcgatcgcg
      301 acaacaaccg tgtcagcgtc agcctggatg aactgaatcg catcgtgcgc ctgtgggaca
      361 gccagaagtc ggcgagcgag cgggaaaacg cgtacaagca tctgttcaat catgtgcacc
      421 cgctggtgat cggtgcggtg gaccgcgcag aaagtctgtc gatcatgctg tgcgaggaga
      481 ttctgtccta ccacatgaaa gattccgcca aaatcaagga aatcgcggtg acgctgaatt
      541 ccaaatatcc gtcgcacgcc tatccgatcc tgtttggtga atcgcgccgt attggcctgc
      601 atgtgaaaat gctggacaag gaagtgaaca ccctgctgct ggagctgaac gagctgtatt
      661 ccgaaatggg tcagcgcgcc accacggatt tcgacgagat ccgttcccac tccaacgaaa
      721 tcctcaacat tctggaaaca tcgggcaaac agattttcta ccagaacgac aaggaatggt
      781 tttaccgcac ggaagagcgg cgctgggtga cgctcaacga caactccaac tggcaccggc
      841 tggaaaagaa aaacggcaag acggtccgct ccaccctgca catttcctga caacctgctg
      901 gcgaggctaa cgtgatcatc gttcaccatc tggaaaattc ccgatcccag cgcgtactct
      961 ggctgctgga ggaactgggc ctgcaatatg aagtgaagcg ctataaccgt gacagcaaaa
     1021 ccatgctggc gccgccggaa ttgcgcgcgg tgcatccgct gggcaagtcg ccggtgataa
     1081 ccgatggtga tcgcgtgatt gcagaatctg gcgccatcat tgaatacctg gtgcggaaat
     1141 acgatccggc gcaccagttc catccggtcg ccggcagtga cgacgaactg gatttcagct
     1201 attggctgca ttatgcggaa ggttcgctga tgccgctgct ggtgatgaag ctgatcttca
     1261 atcatatcca gcgcgcgccg atgccattct ttgtcaagcc ggtagcaaaa ggcatcagtg
     1321 gcaaaacgct gcgatcgttt atccagccca atcttgatac ccatcttgaa tttgtggaaa
     1381 acaggatgcg cggacggcag tggttcgtgg gtgacagctt ttctgcagcc gatgtgcaga
     1441 tgacctttcc gctggaagcc gctgccgcac gtgacaatct cgccaccaag tacccgtcgt
     1501 tgcaggcatt tctgcagcgg gtgcatgaac gccctgctta ccggcgtgcg ctggagcggg
     1561 gtggtcccta tgcgttgatg agctgagcgt gacgcccgtg actgcattgc tatactgaat
     1621 ccacgccacg gaatttcacg ggggaatgca catgctggtc gcaccctgcg cccacgacga
     1681 agaggaccgc ctgcgggcgt tggcgcgcta cgcggttctc gacacatcgc cggaggcgga
     1741 tttcgacgcg gtcacccagc tgctgtcttt tatctgcgac acgccgatca ccctggtgag
     1801 cctggtggac cgcgatcgcc agtggttcaa atcccgacaa ggtctggacg ctacggaaac
     1861 cagccgggac cttgcttttt gcgcccacgc catcctgcaa ccggatccct tgatcgtgga
     1921 ggatgttacc ctcgatccac gttttgcgga caatcctctg gtcctgtcgg ctcccaacat
     1981 ccgtttctat gcaggagcgc cgctgatcac tcacgacggc ttcgcactgg gaacgctttg
     2041 cgccattgat atcaagccac gtaccttctc atcgacccag ttacatgccc tgaccacgct
     2101 ggcacgtcat gttgtcgcga tactggagtt gcgcctgaag ctgcgcgaga ctgaagatct
     2161 gaatcgtgag ctggcgcgcg cacagcgcga actggaagaa aacattgcct atcgctcgcg
     2221 ttttttcgcc accgtcaatc atgaaatgcg cacgccattg aatgcgattg cgggatttgc
     2281 ccagcggctg cagaagcgga tcgtggttga gaccgtgccg gactatgtgc acgaaggcct
     2341 ggatctgatc ggtgctgctg cccgccgtct ggtggatctg gtcagcgatg tgctcgacat
     2401 gtccaaaatt gaagtgggca agatgacact gaatgagcag cctttttcgc ctgcagccct
     2461 gttgcgggaa gtgtgcgaca ctctacaggt tgtggcagaa gaaaaaggcg tattgctgca
     2521 acttcagatc gatcccgccc tgccggcaca gtatgtcggc gatgcgaaga agctgggcca
     2581 gattgcgttg aatctggtgt ccaatgcagt gaagtttacg ccgccgcaaa aacatgtacg
     2641 ggtgctctgc gagccagtcg aaaatggaat gcgcttggtg gtgcaggatg aaggcattgg
     2701 catcgcggag cagaacagaa aaaaagtatt tttgcccttc gagcaatttc acacgacaac
     2761 cgaattgtcc gagaagggaa ccggtctggg tctcgccatc gtaaaaaatc tggtggatct
     2821 catgcatggt actgtaactt tacaaagtga atttggtaaa ggaaccacat tcacgctgat
     2881 tcttcctttc tccgcagtag ccatttcttc cgcgtcagaa taatcttctt tttccaacga
     2941 tggactggca attcgttggc gccctggaaa ttttcttttt tcatccgcag ctatactgaa
     3001 acgcaaggat gcgatgagaa gggctttaac atgaggaagt tttttttcag cgcactaatg
     3061 ctggcagtct ggaatttttc gcttccctct gcatttgcat ttaatggatt cgacaacatg
     3121 tgcggttgga tccgtactgc gccaggcaag gcacgcatcg tttacatgcg gcatgacgga
     3181 gagtggcgaa cgtaccgtct ttatgtgccg caatcctatc agccaaacgc gcctctggtg
     3241 gtggactt
//
LOCUS       DASUEG010000352         5192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1723526, whole genome shotgun sequence.
ACCESSION   DASUEG010000352 DASUEG010000000
VERSION     DASUEG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723526"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VFX11_06555"
     CDS             complement(<1..1046)
                     /locus_tag="VFX11_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4938317.1"
                     /translation="MRESAADSKVKRWVGATLLICFCITGLATWQTVRHNQQAIDAAV
                     TTAAARAANAVTDRLNLYEHGLRGTRGLILALEEQTISRQRFQRYIQAREIHRGFPGA
                     RGFGFIRRVERLNVPLFVREAREDDWPAFSIRELSPHSGERYVIQYIEPVERNSQAVG
                     LDIASETNRREAALAALETGEARLTGPITLVQATGAPSQSFLFLLPIYRTVATPASAG
                     ERLAACLGWSYAPLLMSEVLADLPLDNLQAHLEIRDTTHPDKEELFYTGPIPQDNPAR
                     LNRHVLQREVHGRQWQFTFTAFPAFIQNLHLISPDWIAWSGLLLSLLMATLVGTVRLN
                     HERQRLLFAGQARMA"
     gene            1192..2151
                     /locus_tag="VFX11_06560"
     CDS             1192..2151
                     /locus_tag="VFX11_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4938318.1"
                     /translation="MRTHLLPLVSLVFVLAACQDQHHQALGTLERDRISHTATAAEIV
                     MELPLREGSQVQQGQLLVRLDDTGQKSRVARAEAEVAAAEAELDKLRSGARQEALAAA
                     RANVAGAEASLVESEANYARARTLAQKKLTSEMMLDRERANRDSAEARWQNAREKLRE
                     LTNGSREEDLRRGEANLQAARATLALEQKLLADLTITATRDGVLDSLPWNVGERVTEG
                     SPVAVVLSGKAPFARVHVPEPYRVKINVDDTLVVAVDGLEKPIEGRVRWIASEPSFTP
                     YYALNQEDRARLMYMAEVQLPESAADLPSGLPAQVFLPGAPLP"
     gene            2148..3089
                     /locus_tag="VFX11_06565"
     CDS             2148..3089
                     /locus_tag="VFX11_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938319.1"
                     /translation="MNDVAIAAHGLGRQFGNLKAVDGLDLQVPRQRIYGFLGPNGCGK
                     TTAIRMLTGLLKPTSGEVNVLGLRLPQQANALRYKIGYMTQKFSLYGDLTVQENLQFV
                     AQIYGITGSHQKQRVAELLSIYDLGSQAGQLAGSMSGGQRQRLALAAAVIHKPELLFL
                     DEPTSAVDPENRRLFWEQLFDLCDAGTTILVSTHYMDEAERCHKLAILETGQKRADGS
                     PAELMDNMGAHVVEIVAPELRRLKEQLLTVAGILSAAQLGARLRVLVSRTKGDPVIFL
                     RAQAMIAPDFELQVVRPSLEDVFVTSTGSARQAANGT"
     gene            3086..4255
                     /locus_tag="VFX11_06570"
     CDS             3086..4255
                     /locus_tag="VFX11_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017026081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4938320.1"
                     /translation="MNSQRPTIFGSFSRIRAILFKELRQLSRDRMTFGMVVMIPLVQL
                     LLFGYAINTNVRDIPVGVVDEAKSALSRTLLQLVSATRVVSIKQHFDTVAEAEAALER
                     TDVRAVLVIPQDLPQRLVRHPTIGRGAPPSSDMETSRPVAGWFVDGSDTLIAGAIKSL
                     GNMPLTELLGKPANRTVPTFAVALYYNPEQRTAVNIVPGLVGIILTMTMIMFTSAAIV
                     RERERGNLEMLITTPVHSLELMLGKIIPYILIGFVQVFIILGLGYLIFDVPLAGGLLP
                     LLLATLLFISASLVLGLVISTVAKTQLQSMQMTVFVLLPSILLSGFMFPYEGMPRAAQ
                     VLAEALPATHFMRLIRGVVLKDVEVLAMTWDMGFLLVFTLAGLTVASLRFKKQLD"
     gene            4494..>5192
                     /locus_tag="VFX11_06575"
     CDS             4494..>5192
                     /locus_tag="VFX11_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4938321.1"
                     /translation="MQPINASVRQPMTALVLTAGGARGAYQAGVMKRISELPVYANSA
                     APFSIITGASAGAINGAAIASQHGNFSASAKLLVKLWSNLQASDVYRTDTLSLLQNAG
                     RLALDFTLGGLTGAGRVSALVDSSPLYGFLSQNLSLDGIRRSVREGGLYALAITATGY
                     HSGRAFTFIEGKPGHPLWNKSRRAALSAEISVQHILASAAIPIVFPPVELPCGKSTAY
                     FGDGAMRLVTPLSPA"
BASE COUNT         1002 a         1523 c         1627 g         1040 t
ORIGIN      
        1 gccatgcgcg cctgtccggc aaacagcagg cgctgacgct catggttgag gcgcactgtt
       61 cccacgagcg ttgccatcag cagactgagc agcagtccac tccatgcaat ccagtccggg
      121 gaaatcagat gcaggttctg gatgaacgcg ggaaaggcgg tgaaagtaaa ttgccactgg
      181 cggccgtgca cctcgcgctg cagcacgtgg cgattcaacc tggcgggatt gtcctgggga
      241 atcgggccgg tataaaaaag ttcttccttg tcgggatgcg tagtgtcgcg gatttcaaga
      301 tgggcctgga gattgtccag cggcagatct gccagcacct cgctcatcaa cagtggcgcg
      361 tagctccatc ccaggcaggc ggcaagccgc tccccggcac tggctggcgt ggcgacagta
      421 cggtaaatcg gcaacaggaa cagaaacgat tgtgacggtg ctccggtggc ctgcaccagt
      481 gtaatgggcc ctgtcagtct ggcttcgccc gtctccaacg cagcgagtgc cgcttcccgt
      541 cggttggttt cggaggcgat gtccagcccc acggcctgtg aattccgttc gaccggttcg
      601 atatactgga tgacgtagcg ttcgccggaa tgcggtgaga gttcacgaat ggagaatgcg
      661 ggccaatcgt cctcccgcgc ctcacggaca aacaggggga cattcagccg ttccacccgg
      721 cggataaaac cgaagccccg ggcgcccggg aatccccgat ggatttcgcg ggcctggata
      781 tagcgctgga aacgctggcg gctgatggtc tgttcctcca gggcgaggat aaggccccgg
      841 gtgcctcgca ggccatgctc gtacaggttc agacggtcgg taaccgcgtt ggcagccctg
      901 gccgcggcgg tggttacggc ggcatcgatg gcctgctgat tgtggcgcac cgtttgccag
      961 gtggccagcc ctgtaatgca gaaacagatc agcagtgtgg cgcctaccca gcgcttcacc
     1021 tttgaatcgg ccgctgattc cctcatgcgt attgctccgt cctttcagtc cagaaagtct
     1081 agcggacatt tggcagtgcg tggatagcga tgtgtgccat gccaaccaga tgacaaatcc
     1141 acagcgttgc gatataagaa aacgctcgcc catcatcgcc aatttcccac tatgcgcacc
     1201 cacttgcttc cccttgtttc gctggtattt gtgctggccg cctgtcagga ccagcaccat
     1261 caggccctcg gtaccctgga gcgtgaccgc atttcgcata ccgccacggc cgccgaaatt
     1321 gtcatggaat tgccgctgcg cgaaggcagc caggttcagc agggccagtt gctggtgcgt
     1381 ctcgacgaca ccggccagaa atcccgggtt gcccgtgccg aagccgaagt ggcggctgca
     1441 gaagcggaac tcgacaaatt gcgcagtggc gcgcggcagg aagcgttggc ggctgcccgt
     1501 gcgaacgtgg cgggtgccga agcgtcgctg gtcgaaagcg aagccaatta cgcgcgggcc
     1561 aggacattgg cgcagaaaaa actcaccagt gaaatgatgc tggaccggga acgggccaat
     1621 cgcgattccg ctgaagcgcg ctggcaaaat gcgcgggaaa aactgcgtga actcaccaat
     1681 ggcagtcgcg aggaggatct gcggcgcggc gaagccaatt tgcaggcggc tcgcgccacc
     1741 ctggcgctgg aacagaaact actggcggac ctgaccatca ctgccacacg ggacggagtg
     1801 ctggacagcc tgccgtggaa cgtaggcgag cgggtgaccg aaggcagccc ggtggccgtg
     1861 gtgttgtccg gcaaggcgcc attcgcccgc gtgcacgtgc cggaacccta tcgagtgaaa
     1921 ataaacgtgg acgacacgct ggtcgttgca gtggatggac ttgagaaacc cattgaaggc
     1981 cgcgtgcgct ggattgccag cgagccctcg ttcacgccct attatgcact caaccaggaa
     2041 gaccgtgccc gcctgatgta catggccgag gtgcaattgc cggagtcagc cgccgatctg
     2101 cccagcggtc tgcctgcgca ggttttttta ccaggggcac cgttgccatg aacgacgttg
     2161 caattgcagc gcacggcctc ggccgccagt tcggtaatct gaaagcggtg gacgggctgg
     2221 acttgcaggt gccacgacag cgcatctacg gatttctcgg ccctaacggc tgcggcaaga
     2281 ccacggccat ccgcatgctg accggcttgc tgaaaccaac ttccggtgaa gtcaatgtgc
     2341 tgggactgcg cctgccgcaa caggccaatg ccttgcgcta caagatcggt tacatgacgc
     2401 agaaattttc cctctacggc gatctgaccg tgcaggaaaa cctgcagttc gttgcccaga
     2461 tttacgggat caccggttca caccagaagc agcgcgttgc ggaactgctt tccatctatg
     2521 acctgggcag ccaggccggg cagctcgccg gcagcatgag cggtggccag cgtcagcgcc
     2581 tggcattggc ggcagctgtt attcacaaac cggagctgct gtttctggac gaacccacgt
     2641 cggcggtcga cccggaaaac cggcgcctgt tctgggaaca gctgtttgat ctgtgcgatg
     2701 caggcaccac cattctggtg tcgactcact acatggatga agccgagcgt tgccacaagc
     2761 tggccattct ggaaacgggg cagaagcgtg ccgacggttc gccggcggag ttaatggaca
     2821 acatgggcgc gcacgtggtt gaaattgtcg cgccggaact gcgcaggctg aaagaacaac
     2881 tgctgacggt ggcgggcatt ctgtccgccg cgcaactggg cgcgcgcctg cgggtgctgg
     2941 tgagtcgcac gaagggtgat cctgtgattt ttctgcgtgc ccaggcgatg atcgcaccgg
     3001 atttcgaatt gcaggtggta cgccccagcc ttgaagatgt gtttgtcacc agcaccggca
     3061 gcgcccggca ggcggccaac gggacatgaa cagtcagcgc ccgactattt tcggttcgtt
     3121 ctcacgtatt cgcgcaattc tgttcaagga attgcgccag ctgtcccgcg accgcatgac
     3181 tttcggcatg gtggtgatga tcccgctggt gcaactgctg ctgtttggtt atgccatcaa
     3241 caccaatgtg cgcgacattc cggtgggcgt ggtagacgaa gcgaaaagtg cattgagccg
     3301 caccctgttg cagctggtca gcgccacgcg cgtggtcagc atcaagcagc atttcgacac
     3361 ggtggcggaa gcggaagccg ctctggaacg cacggatgtg cgcgcggttc tggtgattcc
     3421 gcaggatctg cctcagcgtc tggtccgtca tcccaccatc gggcgtggcg cgccgccgtc
     3481 aagcgacatg gaaaccagtc ggccggtggc gggctggttc gtcgatggct ctgatacgct
     3541 gattgccggc gcaatcaaat ccctcggcaa catgccgctg acggagctgc tgggcaagcc
     3601 ggcaaatcgt accgtgccga catttgccgt tgcgctctac tacaatcccg agcagcgcac
     3661 ggcggtgaat atcgtgccgg ggctggttgg catcattctc accatgacga tgatcatgtt
     3721 cacctccgcc gccatcgtgc gcgagcggga gcggggcaat ctggaaatgc tgatcaccac
     3781 gccagtgcat tcactggagt tgatgttggg caaaatcatt ccctacattc tgatcggatt
     3841 cgtgcaggtg tttattatcc tggggctggg ctatctgatt ttcgatgtgc cgctggccgg
     3901 tgggttgttg ccactgctgc tggccacgtt gctgttcatt tccgcaagcc tggtgctcgg
     3961 actggtgatt tccaccgtgg ccaaaacgca attgcagtcg atgcagatga cggtgtttgt
     4021 tctgctgccc tccattctgc tgtccggttt catgttcccg tacgagggca tgcccagggc
     4081 ggcacaggtt ctcgctgagg cgttgcccgc cacccatttc atgcggctga tccgcggtgt
     4141 ggtgctgaag gatgtggagg tgctcgccat gacctgggac atggggttcc tgctggtttt
     4201 cacactggcg ggcctcacgg tggcctcgct gcggttcaag aagcagctgg attgacgggc
     4261 gattccaggg gcgggcggct gactcgattt ggtgcgcatc attttgggga agcagggaaa
     4321 acggacgcaa atggatcact cgctgatttc gcggaatctg aagagccggt tcccatcaac
     4381 gaaatatccg cctgggtggt tgaaaagagt ggattttccg gaagcgtgac ctgccggtac
     4441 gcgtggcaca cattctgcac attccccggg aaaacaggcc gaggaagatc cgcatgcagc
     4501 ccatcaacgc gtccgtccgc cagcccatga cagcgctggt tctgaccgcc ggaggcgcgc
     4561 gcggcgccta ccaggccggg gtcatgaaac ggatcagtga actgcccgtc tacgccaaca
     4621 gcgctgcccc gttttccatc atcaccggtg cttccgccgg cgccatcaac ggggcagcca
     4681 tcgcgtcaca acacggcaat ttcagtgctt ccgccaaact gctggtaaaa ctctggtcca
     4741 acctgcaggc cagtgatgtg tatcgcaccg acacgctgtc actgttgcaa aatgcaggac
     4801 gcctggcgct ggactttacg ctgggaggat tgacgggcgc aggccgggtg agtgcgctgg
     4861 tggattcgtc tccgctgtac ggatttctca gccagaatct gtcgctggac ggcattcgtc
     4921 gctccgtgcg cgaaggcggg ctgtacgcgc tggccatcac ggccaccggt taccactccg
     4981 gccgggcctt tacttttatc gaaggcaaac caggacaccc tctgtggaac aagagccggc
     5041 gagcggcgct gagcgcggaa atttccgtgc agcatattct ggcgtcggcg gcgattccga
     5101 ttgtatttcc accggttgaa cttccttgtg gcaaatccac cgcctatttt ggtgacggtg
     5161 ccatgcgact ggtgacaccg ctgagcccgg cc
//
LOCUS       DASUEG010000353         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1513424, whole genome shotgun sequence.
ACCESSION   DASUEG010000353 DASUEG010000000
VERSION     DASUEG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513424"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFX11_06580"
     CDS             complement(<1..281)
                     /locus_tag="VFX11_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938322.1"
                     /translation="MDGHNRLMNAGQACGEADLGYKHWVPVHGLGSFLLTLALLGLMN
                     PVLADTQAVAQSAKARPKICVVLSGGGARGAAHVGVLKVLEEYRVPIDC"
     gene            complement(329..1360)
                     /locus_tag="VFX11_06585"
     CDS             complement(329..1360)
                     /locus_tag="VFX11_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX4938323.1"
                     /translation="MKILTVLAWVLIGVMLCAVAAWSLLAITYAGPHHDVLRNALSAL
                     PVLATLPVLVALGFRRWRWRALILWLILFVGELWWWSSLTPSNDRAWQTDVAVLPHAT
                     IEGDLVTLHNIRNFDYRSEFDYTPAYYDKTFNIRELTGVDVVAVYWMGPAVAHVFLSF
                     EFGAGNHLAISIETRKEKDEEYSTLKGFFRQYELYYVVADERDVIRLRTHYRHNPVED
                     VYVYRAAGDLDNGRRLFLEYIRRINQLRSEPEFYNTLTTNCTTSIWLNTLVNQQHLQL
                     DWKILVSGYLPELLFEHGRLQGDGLTFAEVQRRAHVNARALAADRATDGAADFSLRIR
                     QNVESLPAE"
     gene            complement(1406..1564)
                     /locus_tag="VFX11_06590"
     CDS             complement(1406..1564)
                     /locus_tag="VFX11_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938324.1"
                     /translation="MILAEKSRDPIIAQNWSLFGLADVRTRQDSGEDAFSWEVVYSGI
                     IAGVGIRF"
     gene            complement(1561..3504)
                     /locus_tag="VFX11_06595"
     CDS             complement(1561..3504)
                     /locus_tag="VFX11_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEX4938325.1"
                     /translation="MKSRSLRPAILAGVLAAGAALPTQAHDAGQATAELLKGAYPDKP
                     LYSPYAGRNYPTRPLFGDTHLHTAFSFDAGAFGARLTPRDAYRFARGEEVVSSSGQPV
                     KLSRPLDFLVVADHSDGMGFFPLLLSGAPSVVSDPQGRKWYEMIHSGQGAAAAIDIIQ
                     NFGKGTISKTIMPMPGTKAYSGAWEETVNAAEAYNDPGRFTAFIGYEWTSNTGGNNLH
                     RNVIFRDNGDKALRVEPYTTMKPLGSDNPRDLWKWMAAYEEKTGGSVLAIAHNGNLSN
                     GLMFPIIESFTGKAVDSTYAQQRAQWERLYEVTQTKGDGEAHPFLSPNDEFADFETWD
                     KGNLDGSVAKSKDMLEYEYARAALKNGLKLEQKLGTNPYQFGLVGSSDAHTGLAAMEE
                     ENFFGKTAPQEPSPHRMTAKFFDNAATGVKIMDWEVSASGYAAVWASDNSRAALWDAM
                     HRKETYATTGSRLVVRFFGGFDYTAADANSRIPAQVGYAKGVPMGGHLSAAPGGKAPT
                     FLVAALKDPIGANLDRYQIIKGWLDSKGELHEKIYDVAWSGDRKPDATTGKLPAVGST
                     VDIANATWTNTIGAPELIAVWQDPDFDPQQPAFYYGRVLEIPTPRWTAYDAKRFGTKP
                     LEGTTMTLQERAYTSPIWYSPGK"
     gene            complement(3579..4553)
                     /locus_tag="VFX11_06600"
     CDS             complement(3579..4553)
                     /locus_tag="VFX11_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX4938326.1"
                     /translation="MKQLLCVLLLLLTGPAWSHEVRPAYLELRETEPGIFAVLWKVPM
                     KGQWQLGIEPLLPSTAQPLTPVLSHLSNNAAIQTWRIAAPDLRGQTIGITGLEHTLTD
                     ALVRIEFANKHNWVKRLTPDQPRVTIPLQPDAWSVSGDYFSLGVEHILLGIDHLLFVL
                     GLLLIASGVTTLVKTITAFTLAHSITLAASALDLIRVPVAPVEAVIALSILFVALEKL
                     HARPGRTSLALRAPWIVAFLFGLLHGVGFAAALGEVGLPQQQIPLALLFFNVGVEVGQ
                     LAFVGTVLVLMAGIRRLWQRIPVWLESIPPYAIGSLAMFWVFDRVAAF"
BASE COUNT          970 a         1436 c         1344 g          956 t
ORIGIN      
        1 atgcagtcga taggcacccg gtattcttcc agcaccttga gtacgccgac atgggcggct
       61 ccgcgcgcgc cgccgcccga aagtacgaca catatcttgg ggcgcgcttt tgcactttgt
      121 gccacagcct gggtatcggc cagcaccgga ttcatcaggc ccagcagcgc cagcgtcagc
      181 agaaatgaac ctaggccatg gacaggcacc cagtgtttgt aacccaaatc agcctcccca
      241 catgcctgcc cggcattcat caatcggttg tgaccatcca ttttttattc ggtaaatggc
      301 ccacagcctt accagcgcgc gggcacaatt attccgctgg cagtgattcc acattctgcc
      361 ggatgcgcag ggaaaaatcc gccgcgccgt ccgtcgcccg gtccgccgct agcgcccgcg
      421 cattgacatg ggcacggcgc tgcacttcgg caaacgtcag cccgtcacct tgcaggcggc
      481 catgttcgaa cagcagttcg ggcaggtagc cgctcaccag tattttccag tccagctgca
      541 aatgctgctg attgaccagc gtattcaacc agatactggt agtgcagttg gtggtcagtg
      601 tgttgtaaaa ctctggctcc gacctgagct ggttgatccg gcggatgtac tcaagaaaaa
      661 ggcgccgtcc attgtcaaga tcaccagcgg cgcgatagac atacacatct tccaccggat
      721 tgtgccggta gtgggtacgc agacggatga cgtcacgttc gtccgcgacc acatagtaaa
      781 gttcgtactg acggaaaaag cccttcagcg tcgagtactc ctcgtctttc tccttgcggg
      841 tctcgatcga aatggccaga tgattgcctg cgccgaattc gaagctgagg aatacgtggg
      901 caacggcggg ccccatccag tacaccgcca ccacatccac ccccgtcagt tcgcggatat
      961 tgaaggtttt atcgtaatag gcgggcgtgt agtcgaactc gctgcggtaa tcgaaattgc
     1021 ggatgttatg cagcgttacc aagtcacctt cgatggtggc gtggggcaat acggccacgt
     1081 cggtctgcca ggcgcggtcg ttggacggcg tgagtgaact ccaccaccac aattcaccca
     1141 caaacagaat cagccacagg atcagtgccc gccagcgcca gcgccggaaa cccagcgcca
     1201 ccagtaccgg caacgttgcc agcactggca gcgccgacag ggcattgcgc agcacatcgt
     1261 ggtgcggccc cgcataggtg atggccagca ggctccacgc cgcgactgca cacaacatca
     1321 caccaatcag cacccaggcg aggactgtca gaattttcac gtgctacgtt ttcctcgatg
     1381 gccggtacaa tcactttctt atctgtcaga accggattcc cacaccggca atgatgccgc
     1441 tgtaaacgac ttcccagctg aacgcatcct ccccggaatc ctgcctggtg cgcacatcag
     1501 ccaatccgaa caggctccag ttttgggcga tgatcggatc tctgcttttt tcagctaaaa
     1561 tcatttaccc gggctgtacc agatgggcga ggtgtacgcc cgttcctgca aggtcatggt
     1621 agtgccttcc agcggtttgg tgccaaagcg cttggcgtca taggcggtcc agcgcggtgt
     1681 gggaatttcc agcacgcggc cgtaataaaa cgccggttgc tggggatcga aatccgggtc
     1741 ctgccacaca gcaatcagtt cgggtgcgcc gatggtgttg gtccaggtgg catttgcaat
     1801 gtccaccgtg ctgcctactg caggcaactt gccggtggtg gcatccggtt tgcgatcgcc
     1861 cgaccacgcg acgtcataga ttttttcatg cagttcgccc ttgctgtcga gccagccctt
     1921 gatgatctgg taacgatcca ggttggcgcc aatggggtct ttcagtgccg ccaccaggaa
     1981 ggtgggcgcc ttgccgccgg gcgcagcgct gaggtgaccg cccatgggaa cacccttggc
     2041 gtaaccgacc tgcgcgggaa tgcggctatt ggcatcggcg gcggtgtaat cgaatccgcc
     2101 aaagaagcgc accaccaggc gggaaccggt ggtggcataa gtttccttgc ggtgcatggc
     2161 atcccacaac gcggctcgac tgttatcgga ggcccacacg gcggcatagc cggaagcaga
     2221 cacttcccag tccatgattt tgacgccggt ggctgcattg tcgaaaaatt tcgccgtcat
     2281 gcggtggggc gatggttctt gcggtgcggt cttgccaaag aaattttcct cctccatggc
     2341 ggccaggccg gtgtgagcat cactgctgcc gacaagtccg aactggtaag gattggtgcc
     2401 cagtttctgt tccagcttga gtccgttttt cagtgccgcc cgcgcgtatt cgtattccag
     2461 catgtccttt gattttgcaa cgctaccgtc caggttgcct ttgtcccagg tttcgaaatc
     2521 ggcgaattca tcgtttggcg acagaaacgg gtgcgcttcg ccatcgccct tggtctgggt
     2581 gacttcgtac aggcgctccc actgggcccg ctgctgggcg tacgtactgt ccaccgcttt
     2641 cccggtgaag gattcgatga tcggaaacat cagaccattg ctgaggttgc cgttgtgggc
     2701 aatggccagc acgctgccac cggttttttc ctcgtaggcc gccatccatt tccacagatc
     2761 gcggggattg tcgctaccca ggggtttcat cgtggtgtag ggctccaccc gcaaggcctt
     2821 gtctccattg tcgcggaaga tcacgttgcg gtgcagattg ttgccaccgg tattggaagt
     2881 ccattcatag ccgatgaaag cggtgaagcg gcccggatcg ttgtaggctt ccgctgcgtt
     2941 gacggtttct tcccaggcgc cactgtatgc tttggtaccc ggcatcggca tgatggtttt
     3001 cgagatggtg cccttgccaa aattctggat gatgtcgatg gcggcggccg cgccctgacc
     3061 ggagtgaatc atttcatacc atttgcgccc ctgcggatcg gacacgaccg aaggcgcacc
     3121 actcagcaac aacggaaaaa aacccatacc gtcagagtga tccgccacca ccaggaaatc
     3181 cagcggacgc gacagcttca ccggctgccc gctggaggac accacttcct cgccccgcgc
     3241 aaaacggtaa gcatcgcgcg gagtcagacg cgcaccgaaa gccccggcat caaatgaaaa
     3301 cgccgtgtgc aaatgggtgt cgccgaacaa tggccgggtc gggtagttgc gcccggcata
     3361 aggggagtac aacggcttgt cgggataggc acccttgagc agttcggccg tcgcctggcc
     3421 ggcatcgtgg gcctgggtcg gcagcgcggc accggcggcc agcacgcccg ccaatatggc
     3481 ggggcgaagt gatcggcttt tcatcctggg tttctccaaa tcaacaaaca gggtacacaa
     3541 atggggatgc tggctgcatc caccattgaa tttttcattc agaaggccgc aacgcggtcg
     3601 aacacccaga acatcgccag cgagccgatg gcgtaaggcg gaatgctttc cagccatacc
     3661 gggatgcgct gccacagacg tcgtattccc gccatcagca ccagcactgt accgacgaag
     3721 gccagttgtc ccacctcgac gcccacattg aaaaacagca gcgccagagg aatctgctgc
     3781 tgaggcaagc ccacctcgcc cagcgctgcg gcgaagccca cgccgtgcag caatccaaac
     3841 agaaatgcga cgatccaggg cgcccgcaac gcgaggctgg ttctgcctgg tcgggcatgc
     3901 agtttttcca gcgcgacaaa caggatgctc aatgcgatga cggcttccac gggcgccacg
     3961 ggcacccgaa tcaggtcaag ggcggatgcc gcaagcgtga tgctgtgcgc aagcgtgaag
     4021 gcagtgatgg ttttcaccag ggtcgtcaca ccgctggcaa tcagcagcag cccgagcaca
     4081 aacagaagat ggtcgatacc caacagaatg tgctccaccc ccagactgaa ataatcgccg
     4141 gacaccgacc aggcatccgg ctgcagcgga atggtcacgc ggggctggtc aggtgtcagc
     4201 cttttcaccc agttgtgctt attggcaaat tcgatccgca ccagtgcgtc agtaagagtg
     4261 tgttccagac cggtaatacc aatggtctgt ccccgcagat caggtgcggc tatgcgccag
     4321 gtctggattg cggcgttgtt gctcagatgc gacagcacag gggtcagtgg ttgcgccgtg
     4381 gatggcagca gaggctcgat gcccagctgc cattggccct tcatcggtac tttccacaac
     4441 acagcgaaaa tgccgggttc ggtttcccgc aactccagat aggccggccg cacttcgtgt
     4501 gaccaggctg gccccgtcag caacagcaac aacacgcaca gcagctgctt catggtgcca
     4561 cctgcgacac ccggtccgga cccatcgcag gactcagtgc ctcatccact tccacctgat
     4621 atttctgcaa cagcgtggca aagtattgct gcctggcctt ttcctgttcg acacgggccc
     4681 attccgccag cacgtgcccg cgcact
//
LOCUS       DASUEG010000354         6291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1785417, whole genome shotgun sequence.
ACCESSION   DASUEG010000354 DASUEG010000000
VERSION     DASUEG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785417"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFX11_06605"
     CDS             complement(<1..600)
                     /locus_tag="VFX11_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4938327.1"
                     /translation="MKQFRGKVAAITGAGSGIGQALAIQLAQAGCDLALSDVNQVGLQ
                     DTVRQVSSTGVRVHVQPLDVANRDAMYAWADTVVAVLGKVNLIFNNAGVALTETVEAM
                     RYADFEWLMNINFWGVVHGTKAFLPHLKASGDGHVVNVSSVFGIIGVPTQSAYNAAKF
                     AVRGFTESLREEMAIEGYPVNVTCVHPGGIKTNIVRSGRM"
     gene            complement(799..2631)
                     /gene="rpsA"
                     /locus_tag="VFX11_06610"
     CDS             complement(799..2631)
                     /gene="rpsA"
                     /locus_tag="VFX11_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX4938328.1"
                     /translation="MRITSGWFSFAFWRFPASYLPAEEGKQKQTTRAVLVACGVNAGL
                     LRRQQQVENMSESFALLFEESLKELDMTPGSIVKGTIISIDDDWVTVYAGLKSEGVIP
                     REQFLSERGVLEIQVGDEVDVALESLEDGWGETRLSREKAKRAESWKRLEKAFEASEV
                     VKGIINGKVKGGFTVDIESVRAFLPGSLVDVRPVRDTGHLEGKELEFKVIKLDAKRNN
                     VVVSRRAVLEAENSAEREALLEKLQEGMEVKGIVKNLTDYGAFVDLGGIDGLLHITDM
                     AWKRIKHPSEIVDVGDEINVKVLKFDRERNRVSLGLKQLGSDPWVEITNRYPEGSRVK
                     ARVTNLTDYGCFAELQEGVEGLVHVSEMDWTNKNVHPSKVVSIGDEVDVIVLDIDEER
                     RRISLGIKQCKPNPWEEFATKFNKNDKISGKIKSITDFGIFIGLDGGIDGLVHLSDIS
                     WDLPGEEAVRNYKKGDEIDTVILSVDPERERISLGIKQLEQDPFAQYVQENDKGTVVK
                     GKVKSVDAKAAVVELTDGVEATLKASEISRDRVEDARVHLKEGDEVEARIISVDRKNR
                     TINLSVKAKDVADEKEAMKSVNQASESAPKTIGDLIKQQLNADK"
     gene            complement(2704..3402)
                     /gene="cmk"
                     /locus_tag="VFX11_06615"
     CDS             complement(2704..3402)
                     /gene="cmk"
                     /locus_tag="VFX11_06615"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX4938329.1"
                     /translation="MAKGPFKIPVITIDGPGGSGKGTIAQRVAQALKWHLLDSGALYR
                     LVGLAASHHGVALDNEASLKILAENMDVQFVVDDPHQPIRIVLEGENVTREIRSEEAG
                     RMASKVAALPGVREGLLQRQHDFREAPGLVADGRDMGTVVFPDADLKIYLTASVDERA
                     SRRFKQLREKGMSANLADLQAAIQARDEQDMNRAVAPLVPADDAVIIDSTDMTIEEVF
                     TELMHHARQKGLIH"
     gene            complement(3395..5626)
                     /locus_tag="VFX11_06620"
     CDS             complement(3395..5626)
                     /locus_tag="VFX11_06620"
                     /EC_number="1.3.1.12"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional prephenate
                     dehydrogenase/3-phosphoshikimate
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4938330.1"
                     /translation="MKNLPLGTVTIVGLGLIGGSFALGLKANRLCREVVGVSRSEATL
                     AKGLELGVIDRAEPDVEKAVRGADLVMLAAPIGTTEALLRKMAPALDGTTIVTDAGSV
                     KGNVAAAAKAVFGGVPSWFVPGHPIAGSERSGVEAAKVDLYRNHKVILTPDNATDPAA
                     LRVVQRLWQAMGADVLLMDVPQHDAVLARTSHLPHLLAFSLVDTLAKDNENQDIFRYA
                     AGGFRDFTRIASSDPTMWHDIFIANRDSMLDVIDQFSAGLDQFRRAVEQQDSQALLGI
                     MTRAKAARDHFTKMLAKRAYTKTMTEKNIVFTAHPGGEVKGSIRVPGDKSISHRSIML
                     GSLAEGVTEVTGFLEGEDSLATLQTFRDLGVTIEGPNHGHVKIYGVGMHGLLPPHGNL
                     YLGNSGTSMRLLSGLLSAQAFDTVLTGDESLSKRPMGRVAKPLREMGAVVETEEKGTP
                     PVRIKGGQQLKGIRYDMPMASAQVKSCVLLAGLYAQGETSVTEPAPTRDHTERMLRGF
                     GYPVKVEGNRITLQGGGKLSATKIDVPADISSAAFFMVAATITPGADLTLEHVGINPT
                     RIGVINILRAMGGKLDVLNEREVGGEPVADIRIRHAPLQGIDIPEDQVPLAIDEFPAL
                     FIAAACAKGTTVLRGAEELRVKESDRIQVMADGLKTLGVKADPTSDGIIIEGGPIGSG
                     IIESHGDHRIAMSFAVAALRSQGDIQIRNCANVATSFPNFVELSRKVGMKLDVAEAGE
                     QHG"
     gene            complement(5623..>6291)
                     /locus_tag="VFX11_06625"
     CDS             complement(5623..>6291)
                     /locus_tag="VFX11_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4938331.1"
                     /translation="HDLKAMAAAITAKTKLIFIANPNNPTGTWFGKAEWEAFMAQVPE
                     SVLVVLDEAYIEYVEDADALDGLQYVNRYPNLIVSRTFSKAYGLAGLRVGYCISHPQV
                     ANVLNRVRQPFNVDSFALAAATAVLDDTDYLQRSRQVNSDGMRQLVAGFKKLGLDYIP
                     SAGNFIVVHVGRDAGPVYQALLKEGVIVRPVANYGMPQSLRISIGLPQENQRFLDALE
                     RVLQ"
BASE COUNT         1200 a         1951 c         1801 g         1339 t
ORIGIN      
        1 catacgaccg ctgcggacga tgttggtctt gatgccgccc ggatgcacgc aggtcacgtt
       61 taccggataa ccttcaatcg ccatctcttc acgcagggac tcggtaaatc cgcgcaccgc
      121 aaacttggcg gcattgtaag cactctgggt tggtacgccg atgatgccaa acacgctgga
      181 cacattgacg acatgaccgt cacccgacgc cttcagatgc ggcaggaacg ccttggtgcc
      241 gtgcaccaca ccccagaaat tgatgttcat cagccattcg aaatccgcat agcgcatggc
      301 ctcgaccgtt tccgtcaacg ctactccggc gttgttgaaa atcaggttca ccttgcccag
      361 cacagctact accgtatcag cccaggcgta catggcatcg cggttggcca cgtccagcgg
      421 ctgcacatgc actcgcacgc cggtggagct gacctggcgc accgtatcct gcagtccgac
      481 ctgattcacg tccgacagcg ccagatcaca gcccgcctgc gccagctgta tcgccagcgc
      541 ctgaccgatg ccggagcccg cgccggtgat ggcggccact tttcccctga actgtttcat
      601 gttgcatccc cacgacaatg ataaagctgc ttatacggct tgcaccgggc aagcgagaaa
      661 gcattcagca tagaccggcc acagaacctc aggcaaccgg ccccacagcg atcccacttc
      721 ccacaaatta cgggtcaata aaaaagggga acggcatgaa accgttcccc ttttttccgg
      781 cagtatccga atagcggatt acttgtcggc gttcagttgc tgcttgatca ggtcgccgat
      841 agtcttcgga gccgattccg acgcctgatt gaccgacttc atggcttcct tctcgtccgc
      901 cacgtccttc gccttcaccg acaggttgat cgtgcggttc ttgcgatcga cgctgatgat
      961 gcgggcttcc acttcatcgc cttctttcag gtgaacgcgt gcatcttcta cgcggtcacg
     1021 gctgatttca gacgctttca gagtggcttc cacaccatcc gtcagttcaa caacagcggc
     1081 cttggcgtca acagacttga ccttgccctt caccacggtg cccttgtcgt tttcctgcac
     1141 gtactgtgca aacggatcct gttccagctg cttgatgccc agcgaaatgc gctcgcgctc
     1201 gggatctaca gacaggataa cggtatcgat ctcgtcacct ttcttgtagt tgcgaacggc
     1261 ttcttcgccc ggcagatccc agctgatgtc ggacaggtga accagaccgt cgatgccgcc
     1321 gtccagaccg atgaagatac cgaaatcagt gatggacttg attttgccgg agatcttgtc
     1381 gttcttgttg aacttggtgg cgaactcttc ccagggattg ggtttgcact gcttgatacc
     1441 cagggagata cgacgacgct cttcgtcgat gtccagaacg atcacatcca cttcgtcgcc
     1501 gatggaaacg actttggacg gatgcacgtt cttgttggtc caatccattt cggaaacgtg
     1561 taccagacct tccacgcctt cctgcagttc ggcgaagcag ccgtagtcag tcaggttggt
     1621 aacgcgcgct ttcacgcggc tgccttcggg gtaacggttg gtgatttcaa cccagggatc
     1681 ggagcccagc tgtttcagac ccagggaaac gcggttgcgc tcgcggtcga acttcaggac
     1741 tttcacattg atttcatcgc ccacatcgac gatttcgctc ggatgcttga tacgcttcca
     1801 ggccatgtcg gtgatgtgca gcagaccgtc gataccgccc agatccacga acgcgccgta
     1861 gtcggtgagg tttttgacga tacctttgac ttccattccc tcttgcagtt tttccagcag
     1921 cgcttcgcgc tcggcgctgt tttctgcttc gaggacggca cgacgggaca caaccacgtt
     1981 gttgcgcttg gcgtcgagct tgatgacctt gaattccagt tccttgcctt ccaggtgacc
     2041 ggtgtcacgc acaggacgga cgtctaccag ggaacccggc aggaacgcac gaacggattc
     2101 gatatcaacg gtgaaaccac ccttgacctt gccgttgatg atgcctttaa caacttcaga
     2161 cgcttcgaag gctttttcca gacgcttcca ggattcggcg cgcttggcct tctcgcggga
     2221 cagacgggtt tcgccccaac cgtcttccag ggattccagt gcaacgtcaa cttcgtcgcc
     2281 cacctggatt tccagtacac cgcgctcgga caggaactgt tcacggggaa tcacgccttc
     2341 ggatttcaaa cccgcgtaca cggtaaccca gtcgtcatcg atggagatga tggtgccttt
     2401 gacgatggaa ccgggggtca tgtccagttc tttcagactt tcttcaaaaa gtagggcaaa
     2461 actttcgctc atgttttcga cctgttgttg gcgccgtaat aaaccggcat tcactccgca
     2521 cgctaccagc accgcacggg ttgtttgttt ctgttttcct tcctcagctg gcaaatacga
     2581 ggctggaaaa cgccaaaaag caaatgaaaa ccaccctgag gtgatcctca tttgcgtttt
     2641 attcctgcgg gaagatcggt gtcttccgtc gtcactctgc agccccgcaa ggctgcgggc
     2701 cgatcagtgg atcaggccct tttgtctggc atgatgcatc agttcggtga aaacctcttc
     2761 tatcgtcatg tcagtgctat caattatcac ggcatcgtca gcgggcacca ggggcgccac
     2821 tgcacggttc atgtcctgct cgtcgcgggc ctgaatcgcg gcctgaaggt cggctaagtt
     2881 agcactcatg cccttctcac gcaactgctt gaagcggcgg ctggcgcgct catccacgct
     2941 ggcggtgagg tagatcttca ggtccgcatc cgggaatacc acagtaccca tgtcacgacc
     3001 gtcggccacc agaccagggg cttctcggaa atcgtgctgg cgttgcagca ggccttcgcg
     3061 cacgcccggc agcgccgcca ctttcgacgc catgcggcca gcttcctcgg aacggatctc
     3121 gcgggtcacg ttttcgcctt ccagcacgat gcggatgggc tggtggggat catccaccac
     3181 gaactgtacg tccatgtttt cagccagaat tttcaggctg gcttcattgt cgagcgccac
     3241 accgtgatgc gaggccgcca gtcccaccag acggtagagc gcaccgctgt ccagcaggtg
     3301 ccacttcaac gcctgggcga cccgctgggc gatggtgccc ttgccggaac cgccggggcc
     3361 gtcaatggta atgacgggga tcttgaatgg gcctttagcc atgctgttct cctgcctctg
     3421 cgacatccag cttcataccc accttgcgtg acagttcaac gaagttgggg aaggatgttg
     3481 ccacattggc gcaatttcgg atctggatgt ccccctggga acgcagcgcc gccaccgcaa
     3541 aggacatggc gatacgatgg tcgccgtggc tttcgatgat gccactgccg atgggtccac
     3601 cttcaataat gatgccatcc gacgtggggt ccgctttcac ccccaaagtc ttgaggccgt
     3661 ccgccatcac ctggatacgg tcgctttcct tgacccgcag ctcctcggcg ccacgcagca
     3721 cggtggtccc cttggcacag gcggcggcaa tgaacagggc cgggaattcg tcgatggcca
     3781 ggggaacctg atcttcaggg atatcgatgc cctgcaatgg tgcatggcgg atgcggatat
     3841 cggccaccgg ctcaccgccc acttcccgct cgttcagcac gtcaagtttg ccgcccatgg
     3901 cgcgcaggat gttaatgacg ccaatccggg tagggttgat acccacgtgt tccagggtca
     3961 ggtcggcgcc gggcgtgatc gtggcggcca ccatgaagaa ggcggcggac gaaatgtccg
     4021 caggcacatc gattttggta gcggaaagct tgccgccgcc ctgcagggtg attctgttgc
     4081 cttccacctt caccgggtag ccgaagcccc gcagcatgcg ctcggtgtgg tcgcgggtgg
     4141 gagccggctc ggtcacgctg gtctcgccct gcgcatacag gcccgccagc aggacgcagg
     4201 atttgacctg ggcgctggcc atgggcatgt cgtaacggat gcctttcagc tgctgaccgc
     4261 ccttgatacg aaccggcggc gtaccctttt cctcggtttc caccacggcg cccatttccc
     4321 gcagcggctt ggccacccgc cccatggggc gcttggacag ggactcatcg ccggtcagga
     4381 cggtatcaaa tgcctgcgcc gagagcaggc cggacagcag gcgcatggat gtgccactgt
     4441 tacccaggta caggttgccg tggggcggca acaggccgtg catgcccaca ccgtaaatct
     4501 tcacgtggcc atggttgggg ccctcaatgg tgacgcccag gtcgcggaag gtctgcaggg
     4561 tggccaggct gtcttcccct tccagaaagc cggtcacttc agtgacgcct tccgccagcg
     4621 agcccagcat gatggagcgg tgggagatgg atttgtcgcc ggggacgcgg atgctgccct
     4681 tcacttcacc gccgggatgg gccgtgaaca cgatgttctt ctcggtcata gtcttggtgt
     4741 acgcacgttt ggctaacatt ttggtgaaat ggtcccgtgc cgccttggcc cgggtcatga
     4801 tgcccagcag ggcctgcgaa tcctgctgct ccaccgcccg gcggaactgg tccagcccgg
     4861 cactgaactg gtcgatcacg tccagcatgg aatcccggtt ggcgatgaag atgtcatgcc
     4921 acatggtggg atcgctggag gcgatgcggg tgaagtcgcg aaaaccgccg gcagcatagc
     4981 ggaagatgtc ctggttttca ttgtcttttg ccagcgtgtc caccagcgag aacgccagca
     5041 ggtgcggcag gtggctggtg cgggccagga cggcatcgtg ctgcggcacg tccatcagca
     5101 gcacgtcggc acccatggcc tgccacaggc gttgcaccac ccgcagggca gcggggtcgg
     5161 tggcattgtc cggggtaagg atgactttat gattgcggta gagatcgacc ttggccgcct
     5221 ccaccccgct gcgttcagaa ccggcgatcg gatgccccgg cacgaaccag gatgggacac
     5281 cgccaaagac ggccttcgca gccgctgcca cattgccttt cacgctgccg gcatcggtga
     5341 cgatggtagt gccgtccagt gccggtgcca ttttccgcaa caacgcctcc gtggtaccga
     5401 tgggcgcagc cagcatcacc agatccgcgc cacgcacggc tttttccacg tccggctcgg
     5461 cacggtcgat cacgcccagc tccagtccct tggccagggt ggcttcgctg cggctcacac
     5521 ccaccacttc ccggcacaga cggttagcct tcaggcccag tgcaaaggat ccgccgatca
     5581 ggccgaggcc gacgatggtc acggttccca acggcaggtt cttcactgga gaacccgctc
     5641 aagcgcatcc agaaagcgct ggttttcctg cggcaaacca atactgatgc gcagggattg
     5701 gggcatgccg taattggcca cggggcgcac gatgacgcct tccttcagca gcgcctggta
     5761 cacgggacca gcgtcgcggc ccacatgcac gacgatgaaa ttgccggcgg agggaatgta
     5821 gtcgagcccc agcttcttga aacccgcaac cagctgacgc atgccatcgc tgttgacctg
     5881 acggctgcgc tgcaggtaat cggtatcgtc cagcaccgca gtggcggcgg ccagggcgaa
     5941 ggaatccacg ttgaatggct gacgcacgcg gttcagcacg ttggccacct ggggatggga
     6001 gatgcagtaa cccacgcgca atccggccag tccatacgcc ttggaaaagg tgcggctgac
     6061 gatcaggttc gggtagcggt tcacatactg cagaccgtcc agcgcatcgg cgtcctccac
     6121 gtattcgata taggcttcgt ccagcaccac cagcacggat tccggcacct gcgccataaa
     6181 ggcttcccat tcggctttac cgaaccaggt gccggtggga ttgttgggat tggcgatgaa
     6241 gatgagcttg gttttcgcag taatcgccgc cgccattgcc ttcaggtcat g
//
LOCUS       DASUEG010000355         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_94652, whole genome shotgun sequence.
ACCESSION   DASUEG010000355 DASUEG010000000
VERSION     DASUEG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94652"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="zipA"
                     /locus_tag="VFX11_06630"
     CDS             <1..915
                     /gene="zipA"
                     /locus_tag="VFX11_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEX4938332.1"
                     /translation="WRTRLRLKIEPVPPGPDLEVTSNPEVGPVRVVRRTEPVIASEVK
                     HKMEDVSVVNAANEARAAENASLDVSMTEDASAEQAASNGDVIADQVDEPVLLTPASV
                     ETASRVAEQQDLFADEPIAPPPDRRHAASRAQALYGAPESVEPVRPVASDSVEKMPME
                     IEQVIALHVVSRGHPFNGEDLLRAVLSYGLRFGDMSIFHRHEQPSGQGKVLFSMAKAV
                     EPGIFDLEAMTGEEVPGVSFFLALPGVNSIIAYDIMVDTARRLANELQGDVLDENQQP
                     LTRQLIEHYRERVQEFERHRLMSRSGGR"
     gene            926..>2404
                     /gene="ligA"
                     /locus_tag="VFX11_06635"
     CDS             926..>2404
                     /gene="ligA"
                     /locus_tag="VFX11_06635"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4938333.1"
                     /translation="MGREEAAREIERLKALIRHHNHRYYVLDDPELPDSEYDRLFNAL
                     RQLESQYPDLVTPDSPTRRVGGEPLAGFATVTHEVPMLSLDNVFSEDEFRDFDRKARE
                     RLGDLLGGPDDLFSSPEIEYCAEPKLDGLAISLLYENGQLVRGATRGDGSTGEDITHN
                     VRTIPSVPLCLMGDTIPLRLEVRGEVVMPKSGFLKLNERAAEAGEKQFANPRNAAAGS
                     LRQLDPRVAAKRPLEFYAYGAVQADGIELPASQYQTLLTLQQLGFRISQEIRHGAGAM
                     FVMEFYADIQRRRDSLPYEIDGVVIKVDSVRWQQELGFVSRAPRWATAFKFPAQEAIT
                     TVEAIEFQVGRTGALTPVARLAPVFVGGVTVSNATLHNFDEIERMDVRVGDSVVIYRA
                     GDVIPKVVRVLHDRRSTATTPVSLPTHCPVCGSDVERPEDEAIARCSGGLFCPAQQKE
                     ALKHFVSRRAMDIEGLGDKWIEQLVDQELIRSSADLYALRRED"
BASE COUNT          477 a          593 c          775 g          559 t
ORIGIN      
        1 tggcggacgc ggttgcggct caagattgag ccggtaccgc cggggcctga tctggaggtg
       61 acatcaaacc ctgaagtggg tccggttcgg gtggttcgtc gtacagaacc ggtgattgcg
      121 tctgaagtga agcacaagat ggaggacgtc tctgttgtca atgctgcgaa tgaagctcgg
      181 gctgcagaaa atgcttcttt ggacgtttcg atgaccgagg acgcgtctgc ggagcaagca
      241 gcctccaatg gggatgtgat tgctgatcag gtggacgaac ccgtattgct gacgcctgcg
      301 tcggtggaga cggcttcacg tgtcgccgag cagcaggatc tgtttgctga cgagccaatc
      361 gctccgcctc cagaccgtcg tcatgccgcc agtcgtgcgc aggcacttta cggggcgcct
      421 gagagtgtcg aaccggtgag gccagtggca tcggattccg ttgagaagat gccgatggag
      481 attgagcagg taattgcttt gcatgtggta tctcgcggac atccgttcaa tggagaggat
      541 ctgctgcgtg cggtattgag ttacggattg cgttttggcg acatgtccat ttttcatcgg
      601 catgagcagc cgtcggggca aggaaaggtt ctgttcagca tggccaaggc agtggagccc
      661 gggatatttg atcttgaggc aatgacgggc gaagaagtac ccggggtgag ttttttcctg
      721 gcactccccg gcgtcaacag tatcatcgct tacgacatca tggtggatac ggcgcggcgt
      781 cttgccaacg agttgcaggg tgatgtgctc gatgaaaacc aacagccgct gacacgacag
      841 ctgattgagc actatcgtga gcgtgtgcag gagtttgagc ggcaccgtct catgtcccgt
      901 tctggtgggc gctgaggaga gttttgtggg ccgggaagag gcagccagag agattgagcg
      961 cctgaaagcg ctcattcgtc atcataacca ccgctattac gttctcgatg atcctgaact
     1021 gccggacagt gagtatgacc ggctcttcaa tgcactcagg cagctggaat cacagtatcc
     1081 cgatctggtc actcccgatt cgccaacccg gcgagtcggg ggggagccgt tggcgggatt
     1141 cgcgacggtg acccatgaag tccccatgct gtcgctcgac aatgtgttca gcgaggatga
     1201 attcagggat tttgaccgaa aggcgcgcga gcgtctcggc gatctgctag ggggtccgga
     1261 tgacctcttc tcttctcctg aaattgagta ttgtgccgaa cctaaactgg atggcttggc
     1321 catatcactg ctttatgaga acggtcagct tgttcgtggc gccacccgtg gtgatggctc
     1381 gacgggtgag gatattacgc ataacgtccg aacaattccg tcggtccccc tgtgtctgat
     1441 gggtgacacc attcccttgc gcctggaggt gcggggtgag gtggtcatgc ccaagagcgg
     1501 ctttctcaaa ctgaatgagc gtgcagcaga ggctggggaa aaacagtttg ccaatccgcg
     1561 taatgcggcg gcgggcagtt tgcggcaact ggatcctcgc gtggcggcaa agcgcccact
     1621 tgagttctat gcctacggcg ctgttcaggc ggacggcatt gagttgcctg cttcgcaata
     1681 tcaaacgctt cttactctcc agcagctggg gtttcgcatc agccaggaga ttcgtcatgg
     1741 tgcgggcgcc atgtttgtca tggagtttta cgcggacatt cagcgtcgtc gggacagcct
     1801 gccttatgaa attgacggcg ttgtcatcaa ggtggacagt gttcggtggc agcaggagct
     1861 tggttttgta tctcgggccc cacgttgggc aacggcgttc aaattcccgg cgcaggaagc
     1921 aataaccact gttgaagcga ttgagttcca ggttggacga accggtgcgc tgacgccggt
     1981 tgcgaggctg gcgccagttt tcgtgggtgg ggtcacggtt agtaatgcga cactccataa
     2041 ttttgatgaa atcgaacgta tggatgtgcg agtcggcgat agcgtggtga tctatcgcgc
     2101 cggtgatgtc attcccaagg ttgtcagggt gttgcatgac cggcgatcga cagcgacgac
     2161 gcctgtcagt ttgccaacgc attgtccggt gtgtggctcg gatgttgagc gcccggagga
     2221 cgaggcgatt gcccggtgct ctggaggtct tttttgccct gcccagcaaa aagaggcact
     2281 caagcacttc gtctctcgtc gtgccatgga tatcgaaggc ttgggggaca agtggatcga
     2341 gcaactggtt gatcaggagc ttatccgctc cagcgcagat ctttatgcgc tacggcgaga
     2401 agat
//
LOCUS       DASUEG010000356         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_421034, whole genome shotgun sequence.
ACCESSION   DASUEG010000356 DASUEG010000000
VERSION     DASUEG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421034"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..567
                     /locus_tag="VFX11_06640"
     CDS             115..567
                     /locus_tag="VFX11_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938334.1"
                     /translation="MNESRVVVESAEGKHPAHEHRVCLRILVTDDSAAVRQFMKAKLV
                     QLAGNAFDVEIDEASSGEEAVTCVEKNPYDLVFLDVVMPGMGGHEACRRIKSIRKTRV
                     AMLSSLKSGADHHLGRMAGCDNYLTKPPQDTDMGAVLRIVYLRKSITP"
     gene            587..2704
                     /locus_tag="VFX11_06645"
     CDS             587..2704
                     /locus_tag="VFX11_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938335.1"
                     /translation="MSAMDEALRAFLVESIESLAHLEEDLVTLERVPQDAATLARAFR
                     SIHTIKGTCGFFGFNRMEQLTHAAEDVLARLRDGQMLMSRELTSALFELIDAVRHMVA
                     NIEQSGGEGEQEYAALIQVFRGFLDGKNVAAQPAPEMAAALEEVDKGAAVADSAIRVD
                     VGLLDKLMNVVGELVLTRNQILQYAGNQSDPVLAGASQRLNLITTELQEGVMKTRMQP
                     ISTVWSAFPRLVRDLEVHTGKKVKLEREGGDTELDRSLLQAIKDPLTHLLRNAVDHGL
                     ESPQERIAAGKPETGLLRLRALHESGYVIVEITDDGAGVNFEAVLNRAISRGLVRQEQ
                     APQMSERDILQLLFLPGFSTAEHVTNISGRGVGLDVVRNNVERVGGSVDIQTRKGSGT
                     TFKVKIPLTLAIIPALVTTCYDERYAIPQVNLLEIVRLDSEERKSRIEPVHDSLVFRL
                     RGKLLPLVDLREVLSLPRRSESSAVHVLVLQADSRSFGLIVDTVRDTEEIVVKPLGRH
                     FKHVNCFAGATIMGDGRVALILDAVAIAQRAHVLTETQQATMWKQAQDVVAKSAHDRH
                     SLLLFRIGSNAQGAIPLSSVKRLEEFPVSAIEYSCGAEVVQYRGTILPLVRVSPLLGI
                     NEAINAELLQVVVHAGDDGEIGLVVDGIVDIVDEEITTHVRKGGRAIIKGSAVIQGRV
                     TDLLDMQKVSLLAAQQVEELINE"
BASE COUNT          573 a          707 c          928 g          617 t
ORIGIN      
        1 gagttttccg aacgaagcgg cgaggagtgc tgctaaaaaa aatgtactgg ttttctggcg
       61 tttcaggggg cgttatgcaa cttgccaagc agtcgacagg gagggatagc gctcatgaat
      121 gagtcaaggg tggtcgttga atcggctgaa ggaaagcatc ctgcgcatga acacagggtt
      181 tgcttgcgta ttctggtaac agatgacagt gccgcagtac gccagttcat gaaggccaag
      241 ctggtacagc tggccgggaa tgcgtttgat gttgaaattg acgaggcttc ttcaggcgaa
      301 gaggctgtca cgtgtgttga aaagaatccg tatgacctgg tgtttctcga tgtcgtgatg
      361 ccaggcatgg gtgggcatga agcctgccgc cgtatcaaga gcattcgcaa gacgcgagtg
      421 gccatgctct ccagtctcaa atcaggtgct gatcatcact tgggccgcat ggccgggtgt
      481 gacaactatc tgaccaagcc accccaggat actgacatgg gggccgttct ccgaatcgtt
      541 tacctgagaa aatccatcac gccttgaggc ctgaggggag ctgttgatga gcgcaatgga
      601 cgaggcgcta cgcgcctttc ttgtcgaaag catcgaaagt ctggcgcatc tggaagagga
      661 tctggtcacg cttgagcgtg taccgcagga tgcggcgacg ttggcgcgag ccttccgttc
      721 catccacacc atcaagggga cctgcggctt ctttggcttc aatcgcatgg agcagctgac
      781 gcacgcggcc gaagatgtgc tggcgcgcct gcgcgacggc cagatgctca tgtcgcggga
      841 actgaccagt gccttgttcg aactgattga tgccgtgcgc cacatggtgg cgaacatcga
      901 gcagagcgga ggcgagggtg agcaggaata tgcggcgctg atacaggttt tccgcggatt
      961 tctggatgga aaaaatgttg cggcgcagcc tgcaccggag atggctgctg cccttgaaga
     1021 ggtggacaag ggggcggcgg ttgccgactc tgccattcgc gtggatgtgg gtttgctcga
     1081 caaattgatg aatgtggtgg gggagctcgt gctcacccgc aaccagattc tgcagtatgc
     1141 cggcaatcag tccgacccgg tactggcagg ggcgtcacag cgtctcaacc tgattactac
     1201 cgagctgcag gaaggggtga tgaaaacccg catgcagccc atcagcacag tgtggagtgc
     1261 ttttccgcgc ctggtgcgtg atctggaagt gcacaccggc aagaaagtaa agctcgagcg
     1321 ggagggcggt gacactgagc tcgatcgcag tctgcttcag gccatcaagg atccgctgac
     1381 acatctgttg cgcaatgcgg tggatcacgg actggagtct ccgcaggagc gtatcgcggc
     1441 gggaaagccg gagacggggc tgttgcgcct tcgcgccctg cacgaaagcg gctatgtcat
     1501 cgtcgagatc acggatgacg gtgccggggt gaatttcgag gcggttctga atcgtgccat
     1561 atcacgtgga ttggtgcgcc aggagcaggc gccgcaaatg tcggagcgtg acatcctgca
     1621 gttgctgttc ctgccgggat tctccacggc ggaacacgtc accaatattt cgggtcgtgg
     1681 agtaggcctg gatgtggttc gcaacaatgt cgagcgtgtg ggtggttccg ttgatatcca
     1741 gacgcgaaag ggatcgggaa cgacattcaa ggtcaagatt ccgctgacac tggccattat
     1801 tccggcgctg gtgacgacct gctacgacga gcggtatgcg attccgcagg tcaatctgct
     1861 tgaaatcgtt cgcctggaca gtgaagagcg caagagccgt atcgagccgg tacatgattc
     1921 gctggtgttc cgtctgcgcg ggaagttgtt gccgctggtg gatttgcggg aagtgctgtc
     1981 gttgccgcgc cgcagcgaga gcagtgcggt gcatgttctt gtgttgcagg cggatagccg
     2041 ctcctttggc ctgattgtcg atactgtacg cgataccgaa gaaatcgtgg tcaagccgct
     2101 gggtcggcac ttcaagcatg tgaactgttt tgcaggcgcc accatcatgg gtgacgggcg
     2161 tgtggcgctc atcctggatg cagttgccat tgcacagcgt gcccatgtgc tcactgaaac
     2221 ccagcaagcc acgatgtgga agcaggccca ggatgtagtg gccaaatcgg cacatgatcg
     2281 ccattcgctg ttgctcttcc gtatcggcag caatgcgcag ggggccatcc ccctgtcatc
     2341 cgtgaagcgt ctggaagagt tcccggtatc cgctatcgag tattcctgtg gtgccgaggt
     2401 cgtccagtat cgtggaacca ttctgccgct ggtgcgtgtc agtccactgc tcgggatcaa
     2461 tgaggccatc aacgccgagt tgcttcaggt ggtggtgcat gcaggggatg acggggagat
     2521 tggtctggtg gtggatggca tcgtcgatat tgtcgacgag gaaatcacga cccacgtacg
     2581 caagggtggg cgcgccatca tcaaggggtc ggcggttatc caggggcgcg tcaccgactt
     2641 gctggacatg caaaaggttt cccttctggc tgcgcagcag gttgaggagc tgatcaatga
     2701 gtgatcaggg attgtattgc acgttctatg tgggacagca gttctatggc attccggtag
     2761 gtgatgtgca ggaggtactg ctggcgcagt tcctgacgcg tgcacccctg gcgccacctg
     2821 ccgtg
//
LOCUS       DASUEG010000357         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1041099, whole genome shotgun sequence.
ACCESSION   DASUEG010000357 DASUEG010000000
VERSION     DASUEG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041099"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFX11_06650"
     CDS             <1..522
                     /locus_tag="VFX11_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020429071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX4938336.1"
                     /translation="EVNADFPARNAAPESAEDTANFLATHMGVDADYLVLPDRHDWFS
                     CVASGSSAPRRMVVCPCSMGTLAAIAHGMSDNLIERAADVVIKERGRLVLVPREAPLS
                     VIHLQNMLVLAQAGAVILPAAPGFYAKPQSIEDLVDGVVQRILDQLDLDIDIAARWVA
                     DASATTTPAFRRD"
     gene            complement(438..1277)
                     /locus_tag="VFX11_06655"
     CDS             complement(438..1277)
                     /locus_tag="VFX11_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX4938337.1"
                     /translation="MNDRNAVFFQVPQTRVRVSAGDVQLPILYYDASALYAFFLVEQE
                     KIRELLLDAELEPGMRVGSKAVVGLACYQYRDTSVGVYNEVGLAVAVSRQGDPLSMGG
                     WRDVLASFTHPEERRSGMYVLDLPVTTALANAAGREIWGFPKFVTPIEFELRDNRFSC
                     SVKYPDTGTPLMTLAGRMGVAVPARPFSLALYSYCNQQLLRTTVNVRGKSQISLPGSV
                     RLQVGDGDHVMVQHLNRLGLNNARPLALLWTDCFQSRLNAGVVVADASATQRAAMSMS
                     RSS"
     gene            complement(1339..3894)
                     /locus_tag="VFX11_06660"
     CDS             complement(1339..3894)
                     /locus_tag="VFX11_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4938338.1"
                     /translation="MGTMIMPAWVDYRGLDADIRACLEAVAGFADVEIARRWRGPEHP
                     LTPEDFTAIAQAADACGLLDSTTDGCGLWSDIPRGSDTAFSVQALRCIARHHTAAAFH
                     LHCLALARVAGLMAGVADVGAGTVFSFSGCGGLGRSALMRWLSAVALSPDDAGLLEDV
                     YGQRERVLFVADATAGVDATAGIIVPMFAQGRMQLAMFATDDAVVQADAQPHGLDGLQ
                     ALVWRPCAAPLWSLPVAANQIASLLAAFQLGLVAIALGASERATARASDYVNVRVQGG
                     RRIIQHDAVALLMVEQDVALQASASRLNDVARLSCTENLAAVVGLKLETLPLLCKGVN
                     AAMQVHGGSGYMRDTGVEDVLRDLNCLRVMGGAPDELALMLATSRFPDAAQIESTLPP
                     HAFASRSLPGFVDAENALAPQVAFRKNPVLRWLASYKSRPAWQRDTDDLPASLCAYRQ
                     QIRAFAGQHLRPVALRLDVQMKTATTRPFDLEVLLREAGRAGLLSDLLPMPFGSVPLP
                     RYRHALAWQQALRAEELARADGGLMLYLSAHNLGVAPVLFSGNLHVYRHVLLPALRAC
                     ETGDPQIFAFAITEPGAGSDAEDGHGAEHSRPGLVATQVKGGWKLRGRKVFISGGDIA
                     RWVVAFAALAGEGFESWTAFLVDTTSVGFHRVRNEHKMGMRASAATELEFDDCFVPDN
                     FVLGGVRKGWALNRATLNFSRLPVAAMAVGFAQQATELATDFVCRVQMGGHPLIHYQN
                     VQLALADMQAETAAIRALVWQYAKAWTPWQARASLAKFHATDRAQKVIERGMDLMGEQ
                     GLLHANEMEKAFRDNRLTRIFEGTNQINRLAVIEDQQTMLLERIARHQQAVRY"
     gene            complement(3930..4331)
                     /locus_tag="VFX11_06665"
     CDS             complement(3930..4331)
                     /locus_tag="VFX11_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEX4938339.1"
                     /translation="MAEAGYSDLPLVYSCSGCSSVAQLANRLALELDREQVAEMSCIA
                     GVGGGVPALVKKALSGRRIIALDGCALRCVHSCLARVNVVANQHYLLTDYGLKKTFHA
                     DFAEDDVVRLKKIIVHNIASDDGVAERLSAV"
     gene            complement(4341..>4939)
                     /gene="ytfE"
                     /locus_tag="VFX11_06670"
     CDS             complement(4341..>4939)
                     /gene="ytfE"
                     /locus_tag="VFX11_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HEX4938340.1"
                     /translation="DFCCGGQHALRDVIAEKNLHAAPILAQLQALQSRKDSSEQDWST
                     APRVELIRHILDHYHAGHRRQLPELIRLARRVEQVHGEREACPVGLANHLLWMQEELE
                     QHMLKEEEILFPLLERGVNGLPIAGPINVMRHEHDQHGEALAVLSDLTNDITLPQGAC
                     NTWRALYAGLRQLRDDLMQHIHLENNILFADAQGCLTA"
BASE COUNT         1013 a         1664 c         1385 g          877 t
ORIGIN      
        1 gaggtgaatg cggattttcc cgcgcgcaat gcggcgcctg aatcagcaga agacactgcg
       61 aattttctgg caacgcacat gggagttgat gcggattatc tggttctgcc cgatcgccac
      121 gactggtttt cctgtgtcgc atccggctcg tcggctccac gccgcatggt ggtgtgcccg
      181 tgcagcatgg gaacgctcgc ggccatcgcc cacggcatga gcgacaatct gattgagcgc
      241 gcagccgatg tggtcatcaa ggaacgcggc aggctggtgc tggtgccacg ggaggcgccg
      301 ctgtcagtga ttcacctgca gaacatgctg gtactggcgc aggccggcgc tgtcattctg
      361 cccgctgcac cgggcttcta tgcgaaaccg caatccatcg aggatctggt ggatggcgtg
      421 gtgcagcgaa ttctggatca gctggacctg gacatcgaca tcgccgcacg ctgggtggca
      481 gacgcgtcgg ccaccaccac acccgcgttc aggcgcgact gaaaacaatc ggtccacagc
      541 aacgccagcg gcctggcatt gttcagcccg agccggttca gatgctgcac catgacatga
      601 tcgccatcac cgacctgcaa acgcacgctg ccgggcagac tgatttgcga cttgccacgc
      661 acattcaccg tggtgcgaag cagttgctgg ttgcaatagg aatacagcgc caggctgaag
      721 ggacgcgccg gcacggcaac gcccatacgg cctgccagcg tcatcagcgg cgtgcccgta
      781 tcgggatatt tcacactgca tgaaaaccgg ttgtcacgca gctcaaattc aatgggcgtg
      841 acaaatttgg gaaagcccca gatttcccgt cctgccgcat tggcgagcgc cgtggtgacc
      901 ggcagatcca gcacatacat gccggagcga cgttcttcag gatgcgtaaa actcgccagc
      961 acatcgcgcc aaccgcccat gctcagcgga tcgccctggc gcgacaccgc caccgccagc
     1021 cccacttcgt tgtaaacgcc caccgaggta tcacgatact gatagcacgc cagcccgacg
     1081 acggctttcg atcccacgcg catgccgggt tcaagctcgg catccagcag cagttcgcga
     1141 attttttcct gctccaccag aaaaaacgca tacagcgcac tggcgtcgta atacaggatg
     1201 ggcagttgca catcgccggc actcacccgc acacgggttt gtggaacctg gaagaacacg
     1261 gcattcctgt cattcatggc gcgcctctct tattgtcata ctggtgcttg ttggatgctg
     1321 aaaccggtat gccggctttt aataacgtac cgcctgctga tgccgggcaa tccgttccag
     1381 cagcattgtc tgctggtctt cgatcactgc cagccggttg atctggttgg tgccttcaaa
     1441 aatccgtgtc agccggttgt cgcgaaacgc cttttccatt tcattggcgt gcagcaggcc
     1501 ctgttcaccc atcaggtcca tgccacgctc aatcactttc tgggcgcggt cggtggcgtg
     1561 gaattttgcc agtgacgccc gcgcctgcca cggcgtccag gctttggcgt attgccacac
     1621 cagtgcgcgg atcgccgctg tttccgcctg catgtctgcc agcgccagct gcacattctg
     1681 gtagtggatc agcggatggc cgcccatctg cacacggcag acaaaatccg tcgccagctc
     1741 ggtggcctgt tgcgcaaacc ccactgccat ggcggcgacc ggcagacggg aaaaattcag
     1801 cgtggcgcga ttcagggccc agcctttacg cacaccgccc aggacaaaat tatccggcac
     1861 aaaacagtca tcaaattcca gctccgtcgc cgcagacgcg cgcattccca ttttgtgttc
     1921 gttgcgcacg cggtgaaatc ccaccgaagt ggtatccacc agaaacgcgg tccaggattc
     1981 aaaaccttcg ccagccagcg ccgcgaaagc caccacccag cgcgcgatgt cgccaccgct
     2041 gataaatacc ttgcggcccc gcagcttcca gccacctttc acctgcgttg ccaccagtcc
     2101 tggcctgctg tgctctgcac catgaccatc ttccgcatcc gatccggcgc cgggctcggt
     2161 aatcgcaaag gcaaatatct gcggatcacc ggtttcgcag gcccgcaatg ccggcaataa
     2221 aacatgacgg tagacatgca ggttgccgga aaacagtacg ggggccacgc ccaggttgtg
     2281 ggcactcagg tacagcatca ggccgccgtc cgcgcgcgcc agttcctcgg cccgcaacgc
     2341 ctgttgccag gccagcgcat gccgatatcg cggcagcggc accgacccaa acggcattgg
     2401 cagcagatcc gacaacaggc cggcgcgccc cgcttcacgc agcaacactt caagatcgaa
     2461 cggccgcgtg gttgccgttt tcatctgcac atccagccgc agagcaaccg gccgcagatg
     2521 ctgtccggca aaggcccgga tttgttgacg ataggcgcac aacgacgccg gcaaatcatc
     2581 agtatcccgc tgccacgctg gcctggattt gtagctggcc agccagcgca gcacgggatt
     2641 tttgcgaaac gccacctggg gtgccagggc gttttcagca tccacgaatc ccggcaggga
     2701 tcgggacgca aaagcgtgcg gcggcaatgt cgactcgatc tgcgcagcat cgggaaatcg
     2761 ggacgttgcc agcatcagcg ccagttcatc cggcgcaccg cccatcaccc gcaggcagtt
     2821 caaatcacgc agcacatctt ccacgccggt gtcgcgcatg tagccactgc caccgtgcac
     2881 ctgcatcgcc gcgttcacgc ccttgcacag cagcggcagc gtttccagtt tcaggccgac
     2941 aacggccgcc aggttttcgg tgcaggacag tctggccacg tcattgagac gcgacgcaga
     3001 cgcctgcaat gccacatcct gctccaccat cagcaaggca acggcatcgt gctggatgat
     3061 gcgacgacca ccctgcaccc gcacgttgac ataatcgctg gcccgcgccg tcgcccgttc
     3121 actggcgccc agcgcaatcg cgacgaggcc cagctggaac gccgccagca gactggcaat
     3181 ctggttggcc gcaacgggaa gcgaccacaa cggcgcagca caggggcgcc acaccagcgc
     3241 ctgcaggcca tcgagaccgt gcggctgtgc gtccgcctgc acgaccgcat cgtcggttgc
     3301 aaacatcgcc agctgcatcc gtccctgcgc gaacatcggc acaataatgc cggcggtagc
     3361 atcaacacct gcagtcgcat cggccacaaa caacacccgc tcacgctggc cgtaaacatc
     3421 ttccagcaaa ccggcatcat ccggcgacag cgcaaccgca ctcagccagc gcatcagcgc
     3481 cgagcgtccg aggccaccac aaccagaaaa tgaaaaaaca gtgccggctc ccacatccgc
     3541 cacgcccgcc atcaaaccgg caacacgcgc cagcgccagg caatgcaggt gaaacgcagc
     3601 agccgtatgg tgacgggcaa tgcaacgcaa agcctgcacg gaaaaggcag tatcggaacc
     3661 gcggggaata tcgctccaga gtccgcagcc gtccgtggtg gaatccagca ggccgcacgc
     3721 atccgccgcc tgtgcgatgg cagtgaaatc ctccggcgtc agcggatgct ccggcccgcg
     3781 ccagcgccgc gcaatttcga cgtcggcaaa accggccacc gcctccagac aggcgcggat
     3841 atcagcgtcc aatcccctgt aatccaccca cgcaggcatg atcattgtgc ccattacgca
     3901 ccccatgacg tactttcatt cgaattcgat cagacagcgc tcaggcgttc tgccacgccg
     3961 tcgtcagacg caatattgtg gacaatgatt tttttcagcc gcaccacgtc gtcttccgca
     4021 aaatccgcgt ggaaggtttt tttcaggccg taatcggtga gcagataatg ctggttcgcc
     4081 accacattca cccgcgccag acagctgtgc acgcagcgca gcgcgcagcc gtccagcgca
     4141 atgatcctgc gtcccgacaa cgcctttttc accagtgcgg gcacaccgcc gcccacaccg
     4201 gcaatgcacg acatttccgc cacctgctca cggtccagct ccagcgccag acgattcgcc
     4261 agttgcgcga cgctggaaca gccagagcag gaatacacca acggcagatc ggaataacca
     4321 gcctccgcca tgatgcctcc ttacgccgtg aggcagccct gcgcgtcggc gaacaggatg
     4381 ttgttttcca gatgaatgtg ctgcatcagg tcatcgcgca actgcctcag cccggcgtac
     4441 agcgcgcgcc aggtattgca ggcgccctgg ggcagggtga tgtcattggt aagatcgctg
     4501 agcaccgcca gcgcctcgcc gtgctggtcg tgttcatgcc gcatcacatt gatcgggccg
     4561 gcgattggca gaccgttgac gccgcgctcc agcagcggga acaggatttc ctcctccttc
     4621 agcatgtgct gctccagttc ctcctgcatc cagagcaggt gattggccag ccccaccggg
     4681 caggcttcac gctcgccgtg cacctgctcc acccgccgcg ccaaccggat cagttccggc
     4741 agctgacgcc ggtgtccggc atggtaatgg tcgagaatat ggcggatcag ctccacccgg
     4801 ggcgcggtgg accagtcttg ctcactgctg tccttgcgcg attgcagggc ctgcaactgg
     4861 gccaggatgg gcgctgcatg caggttcttt tcggcgatca catcgcgcag tgcatgctgc
     4921 ccgccgcagc agaaatcca
//
LOCUS       DASUEG010000358         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1822602, whole genome shotgun sequence.
ACCESSION   DASUEG010000358 DASUEG010000000
VERSION     DASUEG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822602"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFX11_06675"
     CDS             complement(<1..554)
                     /locus_tag="VFX11_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017963779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4938341.1"
                     /translation="MSYKGIILAGGTGTRLHPLTCSVSKQLMPVYDKPMIYYPVSTLM
                     MARIRDVLIITTPRDREAFEHLLGDGKQWGMSFQYAEQPSPDGLAQAFLIGESFLAGS
                     NACLVLGDNVFYGSGLPMVLQNATRRTHGATVFGYYVRDPQRYGVVAFDRQGNVTSIE
                     EKPAQPKSSYAITGIYFFDQDVVD"
     gene            820..2253
                     /locus_tag="VFX11_06680"
     CDS             820..2253
                     /locus_tag="VFX11_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4938342.1"
                     /translation="MWREVQVLLIDDNASRRHDMKVVLDFVGEATFAAASTDWKAVVG
                     EEPGAHFSCAFLGDVPGVVKLAKSLLEWEPTLPIVLMNGTELPTDADDDVARAVIAQI
                     ETPPLYTKMMDTLHRAQVYRDQVSSDPVRAHRREAQLFRSLVGTSRSVVKVRELMAQV
                     ADKDVSVLITGESGTGKEVVSRNLHYHSHRRHKPFVPVNCGAIPQELLESELFGHEKG
                     AFTGAITSRPGRFELAQGGTIFLDEIGDMPLHMQVKLLRVLQERTFERVGGTKTIEAD
                     VRVIAATHQNLEKLIEEGKFREDLYYRINVFPIEMPPLRERTEDIPLLLNELVARIEN
                     EKRGSIRFNSAAIMALCRHEWPGNVRELANLVERLVITHPYGVIGVSDLPKRFRHVDD
                     LDEERELPEEVAPANGGPGYVSMDAPALLPVNGIDLKEYLNNLEFNLIKQALDDCNGV
                     VARAAEKLKIRRTTLVEKMRKYELGRA"
     gene            2417..>3348
                     /locus_tag="VFX11_06685"
     CDS             2417..>3348
                     /locus_tag="VFX11_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4938343.1"
                     /translation="MSAIAAEKKFATAGSFERIASQLNRSYHELESRIGELQHELAQS
                     DQERLREHQAREKLAEMLDVILGVMPVAVIVLDGRGVVAQANAIAANLLGHDLPGQRW
                     IDVIRQYFAPTFTDGHEVALKNGRLLSLATQSLKEEPGQIIVLTDQTETRRLQETLNH
                     HRKLSEMGRMTASLAHQIRTPLSAATLYADHLASPQLSEDRRVKYAGKIKARLAQLGQ
                     QVQDMLIFSRGGVVLDECIDSTGLVTRLQHQLEEICEQQHASCVVESDALSGRVRCNQ
                     ELLTSAFSNLVENAIQACLAQNVKPVLQLSCRLDA"
BASE COUNT          729 a          902 c         1027 g          690 t
ORIGIN      
        1 atgtccacca cgtcctggtc aaaaaaatag atgccggtga tggcgtagga cgatttgggc
       61 tgcgcaggct tttcttcgat gctggtgaca ttgccctggc ggtcgaaggc caccacgccg
      121 taacgctgtg gatctcgcac atagtagcca aacacggtgg cgccgtgggt gcgccgggtg
      181 gcgttctgca acaccatcgg caggcccgag ccgtagaaaa cattgtcacc cagcaccagg
      241 cacgcgttgc tgccggccag aaaggattca ccgatcagga atgcttgcgc cagaccgtcc
      301 ggcgacggct gttcggcata ctggaaggac atgccccact gcttgccgtc tcccagcaaa
      361 tgttcgaagg cttcacgatc gcgcggcgtg gtgataatca gcacgtcgcg gattctggcc
      421 atcatcaggg tggagaccgg atagtaaatc atgggcttgt catacacagg catcagctgc
      481 ttgctgacgc tgcaggtaag aggatgcagg cgggtaccgg tgcctcctgc cagtatgatg
      541 cccttgtaac tcatgatggc tgcatttccg tatataacga acggttcaga agttgcgcta
      601 catatataaa agtagatgag ctttgtccgc tgtccagacg gaaaaaccgc tttggcttga
      661 cggatatgcc aaagctgact cgaattggtc aaaatattca cgccattaat ttgaccaatc
      721 aggggatcgt tgcctttact gagtattacg cataacaacc ggcgtcaaaa ttcgaacatg
      781 aacggagagt ggcgggagcc cgccaatact gagaacagca tgtggcgaga agtacaagtc
      841 ctgttgatcg atgataacgc cagccgtcgc catgacatga aagtcgttct ggatttcgtg
      901 ggcgaagcga cgttcgctgc cgcttccacc gactggaaag cagtggtggg cgaggagccg
      961 ggcgcgcatt tttcctgtgc ttttctgggt gatgtgccag gcgtggtgaa actggccaag
     1021 tccctgctcg agtgggaacc cacgttgccc attgtgctta tgaatggtac cgagctgccg
     1081 accgatgctg acgacgacgt tgcgcgcgct gtgatagccc agattgagac gcctccgctt
     1141 tataccaaga tgatggatac gttgcaccgt gcccaggtgt atcgggatca ggtctcgtcg
     1201 gatcccgtca gggcccaccg tcgcgaggcg cagttgttcc gcagcctggt aggcaccagc
     1261 cgctccgtgg tgaaagtgcg cgagttgatg gcgcaggtgg ccgacaagga tgtcagtgtg
     1321 ctgatcaccg gtgaatccgg cacgggcaag gaagtggtgt cgcgcaatct gcactaccat
     1381 tcccaccgtc gtcacaagcc gttcgtgccg gtgaattgcg gcgcgattcc gcaggagttg
     1441 ctggaaagcg agctgttcgg tcatgagaag ggcgccttta ccggcgccat caccagccgg
     1501 cccgggcgtt tcgaactggc tcagggcggc accatttttc ttgatgaaat cggcgacatg
     1561 ccactgcaca tgcaggtgaa actgttgcgt gtgctgcagg agcgcacctt cgagcgggta
     1621 ggtggcacca agaccatcga ggccgatgtg cgcgtcattg ccgccacgca ccagaatctg
     1681 gaaaagctga tcgaggaagg caagttccgc gaggacctgt actaccgcat caacgttttc
     1741 cccattgaaa tgccaccgct gcgtgagcgc accgaagata ttccgttgct gctgaatgaa
     1801 ctggtggcgc gcatcgaaaa tgaaaaacgc ggttccatcc gtttcaattc cgctgccatc
     1861 atggcgctgt gccggcacga gtggccaggc aacgtgcgcg agctggcgaa tctggtggag
     1921 cgtctggtga ttacgcatcc gtatggtgta attggcgtgt cggatctgcc aaaaaggttc
     1981 cggcatgtgg acgatctgga cgaagagcgg gaattgccgg aagaggtggc accggcgaac
     2041 ggtggccccg gttatgtcag catggatgcg ccagcgctgc tgccggtgaa cggcattgat
     2101 ctgaaggaat acctgaacaa tctggaattc aatctgatca aacaggcgct ggatgattgt
     2161 aacggtgtgg tggcgcgggc ggcggagaag ctgaaaatac gccgcaccac actggtggag
     2221 aaaatgcgca agtacgaact gggccgggcg taacgcccgg ttctgctttc cgccaatcgc
     2281 aaagcctgac aaacaattga ctattcccac cggtacttct tttatatcct cgctaaccgc
     2341 ttgaattccc gctgttttga taatctggca cagcgcttgc cttgagtcct gcaactgtca
     2401 aaagttggag cattccatga gcgccattgc agcggaaaaa aaatttgcca ccgccggttc
     2461 cttcgaacga atcgccagcc agctcaaccg ttcctatcac gagctggaaa gccggatcgg
     2521 tgagctgcag catgaattgg cccagtccga tcaggagcgt ctgcgcgagc atcaggcccg
     2581 ggaaaagctg gcggaaatgc tcgatgtgat cctgggtgtg atgccggtgg cggtgattgt
     2641 gctggatggt cggggcgtgg tggcgcaggc caacgccatt gctgcaaacc tgctgggcca
     2701 tgatttgccg gggcaacgct ggatcgacgt gatccgccag tatttcgcgc ccaccttcac
     2761 agatggccat gaagtggcac tgaaaaatgg ccggttgctg agtctggcca cccagtcgct
     2821 caaggaggaa ccgggccaga tcattgtgct gactgaccag actgaaaccc ggcgtctgca
     2881 ggaaaccctg aatcatcatc gcaagctgtc ggaaatggga cgcatgacag cgtcgctggc
     2941 ccatcagatt cgcacgccgc tgtcggctgc caccctgtac gccgatcatc tggcgtcgcc
     3001 gcagctgagc gaggaccgcc gtgtcaaata cgcgggcaag atcaaggcgc ggctggcgca
     3061 gttaggacag caagtgcagg acatgctgat tttctcccgc ggtggggtgg tgctggatga
     3121 gtgcatcgac agcacaggtc tggtgacccg cctgcaacat cagctggagg aaatatgcga
     3181 gcagcagcat gccagctgcg tggtggaatc ggatgccctg agtggccggg tccgttgcaa
     3241 tcaggagctg ctcaccagcg ctttcagcaa tctggtggaa aacgcgattc aggcctgtct
     3301 ggcccagaat gtgaaaccgg tgctgcagct gtcctgccgt ctggacgc
//
LOCUS       DASUEG010000359         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_817374, whole genome shotgun sequence.
ACCESSION   DASUEG010000359 DASUEG010000000
VERSION     DASUEG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817374"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFX11_06690"
     CDS             <1..1100
                     /locus_tag="VFX11_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HEX4938344.1"
                     /translation="AFMQRHPRAIALFSFGSGVASFFLVNRQKNSAGLLAIVLLVSWL
                     WLMLENLVTKKIARRFGLEIPPALLRYGTQMIHQESLFFVLPFFFVTTTWNSGQSLFT
                     GLLVGAALMSIIDPVYNHWLAVRRLAYMAFHTLALFAVMLTALPVILHLTTDETYRYA
                     TAATVVLAIPTLFRLVPEGGKWQGLLRFVLMIAVLAGGLWAGRQWVPPATLWLTDVHV
                     TFALDGSQRTPGAGIKTLTEAQLHQQGLYAYTAIRAPRGLGEQVFHVWLHEGQEVDRI
                     PLKIRGGREKGYRTWSHKTNFPEQVTGDWQIRVVTEGGQLIGVVRFNVTPGAMVPVDR
                     IPQASGEIAAPPAADSADFLLKDPAGTTINP"
     gene            1127..>2541
                     /locus_tag="VFX11_06695"
     CDS             1127..>2541
                     /locus_tag="VFX11_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4938345.1"
                     /translation="MVSTSLPIRLCRLTFLARWLAGPAVLAWPLAVFAVHSPGEGLFE
                     LSMEELLEVKITSSTLHDESLKTVPSSMTVFTRADIRRLGLQNLRQLTSLVPGYQGSR
                     VDDSVLSYPMSSRGRRVGASGSEVLVLIDGQRLNNDWAGGAGQHTQLISIENVERVEM
                     IRGPGSALYGSNAMMGVINIITRSEREVVVEAGSHQHRHASAQWRAESERGQLEVYAR
                     DTRSEGEDLTLFEPFPSPATPEWVESRDPFRANDLYLHGSLGEFSINAMATSRDMQQY
                     YVAGYVDNRSNARDTRTDFVNLAWRHNFTDQLKLEGRVYQIDRELDIRAAVSLVPYFV
                     LSGDTSERELGTQWILQGDSDRSNWLAGWEWRQPELTDTGYQFGPPSNPAQVQALQAE
                     EDGRTIQGLFGQYQYRVNADLELIAGLRRDDYSDFGDHLSPRLGLVQQVGDSETFKLL
                     YSEAFRAPSRLETSVISPEFQ"
BASE COUNT          444 a          763 c          832 g          502 t
ORIGIN      
        1 tcgcctttat gcaacgccat ccccgcgcca ttgcgctgtt cagcttcggc tccggtgtgg
       61 ccagtttttt cctggtgaac cggcagaaaa actcggcggg tctgctggcg atcgtgctgc
      121 tggtgagctg gctgtggctg atgctggaaa acctggtgac gaaaaagatc gcccgccgtt
      181 tcggcctgga aattccgccg gcgctgctgc gctacggcac ccagatgatt caccaggaaa
      241 gtctgttctt cgtgctgccg ttctttttcg tcaccaccac ctggaacagc gggcagtcgc
      301 tgttcaccgg gctgctggtg ggggcggcgc tgatgtcgat catcgatccg gtttacaacc
      361 actggctggc ggtgcggcgg ctggcctaca tggcgttcca caccctggcg ctgtttgcgg
      421 tgatgctgac ggcgctgccg gtgatcctgc acctcaccac tgacgaaacc taccgttacg
      481 ccaccgccgc caccgtggtg ctggcgattc ccacgctgtt ccggctggtg cccgagggcg
      541 gcaaatggca gggcctgctg cgtttcgttc tgatgatcgc ggtgctggcg ggcgggctgt
      601 gggcagggcg tcagtgggtg ccgccggcca cgctctggct caccgacgtg catgtgacct
      661 ttgcgctgga tggcagccag cgaacgcccg gcgccggcat caaaaccctt accgaggcgc
      721 aactgcacca gcagggcctg tatgcctaca cggcgattcg tgcaccccgg ggcctgggcg
      781 agcaggtgtt tcatgtgtgg ctgcacgagg ggcaggaagt ggaccgcatt ccgctgaaaa
      841 tcaggggcgg gcgcgagaag ggctatcgca cctggagcca caagaccaat ttccccgagc
      901 aggtgactgg cgactggcag atccgggtgg tgaccgaagg tggtcagttg attggtgtgg
      961 tgaggttcaa cgtgacgcct ggcgcaatgg tcccggtcga cagaatcccg caagcgtctg
     1021 gcgaaatcgc tgcgccgcca gctgctgatt ctgccgattt tttgttaaaa gaccctgcag
     1081 gaactacgat taatccgtga gatatccgca cgggctctga tatccaatgg tgagtacctc
     1141 cttgccgatc cggctttgcc ggctgacatt tctggcgcgc tggctggctg gcccggcagt
     1201 tctggcatgg ccgcttgctg tgtttgcggt gcactcgccg ggggagggtc tgtttgaact
     1261 ctccatggag gaactcctcg aagtaaaaat cacctcctcc accctgcacg atgaaagcct
     1321 gaaaaccgta ccttcctcga tgaccgtctt cacccgcgcc gatatccgcc gcctgggcct
     1381 gcagaacctg cgccagctga ccagtctggt gcccggctat cagggttccc gggtggacga
     1441 ttcggtgttg agttacccca tgtccagccg tggccggcgg gtgggcgcca gcggcagcga
     1501 agtgctggtg ctgatcgacg gccagcgcct gaacaacgac tgggctggcg gcgccggtca
     1561 gcacacccag ctgatttcca tcgagaatgt ggagcgggtg gaaatgatcc gtggacccgg
     1621 ttcggccctg tatggcagca acgccatgat gggggtgatc aacatcatta cccgcagtga
     1681 gcgggaagtg gtggtggagg caggaagtca ccagcatcgc catgccagtg cccagtggcg
     1741 ggcggaaagc gagcgcggtc aactggaagt ctatgcccgg gatacccgca gcgaaggcga
     1801 ggatctcacg ctgttcgagc ccttccccag cccggccacg ccggagtggg tggagagccg
     1861 tgatccgttc cgcgccaatg acctgtacct gcatggcagc ctgggtgaat tctccatcaa
     1921 cgccatggcc accagccggg acatgcagca gtactatgtg gcgggctatg tggataaccg
     1981 cagcaatgcc cgcgacaccc gcaccgattt cgtcaacctg gcctggcgcc acaattttac
     2041 cgatcagctg aagctggaag ggcgcgtcta ccagatcgac cgcgagctgg acatccgcgc
     2101 ggcggtttcg ctggttccct atttcgttct gtcgggtgac accagcgagc gcgagctggg
     2161 cacccagtgg attctgcagg gtgatagcga tcggtcgaac tggctggcag gctgggaatg
     2221 gcgccagccg gagctgacgg acaccggcta ccagtttggt ccgccctcca atcccgcgca
     2281 ggtgcaggcg ttgcaggccg aggaggatgg ccgcaccatc cagggcctgt ttggccagta
     2341 ccagtatcgc gtgaatgcgg atctggaatt gattgccggc ttgcgccgcg acgattattc
     2401 ggacttcggc gatcacctga gcccgcggct gggcctggtg cagcaggtgg gcgacagcga
     2461 gaccttcaaa ctgctgtatt ccgaagcgtt tcgtgcgccc agccggctgg agacgtcggt
     2521 gatcagcccg gaattccaga g
//
LOCUS       DASUEG010000360         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1046143, whole genome shotgun sequence.
ACCESSION   DASUEG010000360 DASUEG010000000
VERSION     DASUEG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046143"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFX11_06700"
     CDS             <1..330
                     /locus_tag="VFX11_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938346.1"
                     /translation="TRDNFGIRNNSNGPLRITCPVIVDEADNVAGTNGVWFNTSIAGT
                     SCTLYSMAANGAGVNAVVQSQFAAGGPGWVAIPNIAADNFWGSYSMICTLPAGGRLNS
                     ISIRENP"
     gene            complement(403..1260)
                     /locus_tag="VFX11_06705"
     CDS             complement(403..1260)
                     /locus_tag="VFX11_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4938347.1"
                     /translation="MDAIVKKDIVHFDPPSLRAVEKATAFSRFYFNPQYFGLENVDAL
                     KPALYVSNHSIYGVLDSPLLFEKLFKDKNIVLRSLGDHFHFEVPLWRDIMIEGGAVPG
                     TRENCAHLMEAGEHILVYPGGGREVAKRKGEEYKLTWKTRTGFAYMAMKYQYPVIPVA
                     ALGADDAYDVVYDAYDFMKNPVGRWLLKNKTVADKLRSGDMLMPIAKGIGPTLIPRPE
                     KFYFGFGAPISPAAFKGKEDDKEMQWQFRKQIMDALEGELNKLRLHRQRDTKVSLLRK
                     LLTKKKNAQ"
     gene            complement(1290..2081)
                     /locus_tag="VFX11_06710"
     CDS             complement(1290..2081)
                     /locus_tag="VFX11_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4938348.1"
                     /translation="MFLESNYTEQFEKVDNHTKHLLEVALQGIPPLGAALTYECFEDL
                     YAADANRDCVFVVEEGAIGHQQDGKTLFHYDAGDLVGMTQGVEMPRAKYYAESPVLLR
                     PYNRDALWAAAMANTDKAREWGTYLIAEWGRQTAVIAAIEPEVDRTSLGFQTFKPGET
                     IIQEGTESDTVYSIVEGHAEVFVNNIKVGEVLEDEIFGALSLLTNSPRTATVIADARC
                     LVMVVPRHQFETMIKNHPRICLSLMENMARQIVSLNKQVTAAKNP"
     gene            complement(2218..>2618)
                     /locus_tag="VFX11_06715"
     CDS             complement(2218..>2618)
                     /locus_tag="VFX11_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX4938349.1"
                     /translation="MVKFFRDGLQEMAGNGVDLLFCNRQEALFWAETDSIEEAAERLK
                     TTAKTFAITLGSEGALVFDGQDTHLVPATPVKAVDTNGAGDMFAGAFLYGLTHGYSYA
                     QAGALATKAAARLVTQFGPRLKPEEHKPLL"
BASE COUNT          474 a          747 c          817 g          580 t
ORIGIN      
        1 acccgtgaca acttcggtat ccgcaacaat tccaatgggc ctttgcggat cacctgtccg
       61 gtcatcgtgg acgaggcgga caacgtagcc ggcaccaacg gtgtttggtt caacacatcg
      121 atagcgggaa ccagttgcac gctctacagc atggcggcca atggggccgg tgtgaatgcg
      181 gtggttcagt cccagttcgc tgccggtggg cccggctggg tggcgatacc caatattgcg
      241 gcggacaatt tctggggcag ctattcgatg atctgcacgc tgccggcggg tggccggctc
      301 aacagtattt ccatccggga aaatccctga gaccgcgggc gccaaacaaa agggcgatgc
      361 cgtcaccggt atcgcccttt tcccatctgc gcacgatgaa agttactgcg cgttcttttt
      421 cttggtcagc agtttccgca gcaggctcac cttggtgtca cgctgccggt gcaggcgcag
      481 tttgttcagc tcgccttcca gtgcatccat gatctgcttg cggaattgcc actgcatttc
      541 cttgtcgtct tccttgccct tgaacgccgc cgggctgatg ggcgcaccaa agccgaaata
      601 gaatttttcc gggcgcggaa tcagggtggg gccgatacct ttggcgatgg gcatcagcat
      661 gtcgccgctg cgcagcttgt ctgccacggt cttgtttttc agcagccagc ggccaaccgg
      721 gtttttcatg aaatcgtagg cgtcgtacac cacgtcgtag gcatcgtcgg cgcccaatgc
      781 tgctaccgga atcaccggat actggtattt catcgccatg taggcgaagc cggtacgggt
      841 tttccaggtc agtttgtatt cctcgccctt gcgcttggcc acttcgcggc caccgcccgg
      901 gtacaccaga atatgttcac cggcttccat caggtgcgca caattttccc gggtaccggg
      961 cacggcgccg ccttctatca tgatgtcgcg ccacagcggc acttcaaaat gaaagtggtc
     1021 gcccaggctg cgcagcacga tgttcttgtc cttgaacagc ttttcgaaca gcagcggcga
     1081 atccagcaca ccataaatgg aatggttgct gacatacaac gccggtttca gcgcgtcgac
     1141 attttccagt ccaaaatact gcgggttgaa atagaagcgc gaaaacgcag tggctttttc
     1201 cacggcgcgc agcgacggcg gatcgaaatg cacgatgtct tttttcacga tggcatccat
     1261 gagcgtacct ctacaactga tgcgatggtt cagggattct tggcggcagt gacctgtttg
     1321 ttgagcgaca cgatctggcg cgccatgttt tccatcaggc tcaaacagat gcgcgggtgg
     1381 ttcttgatca tggtttcaaa ctggtgacgc ggtaccacca tcaccagaca gcgtgcgtcg
     1441 gcaatcacgg tggcggtgcg ggggctgttg gtgagcagcg acagcgcgcc gaagatttca
     1501 tcctccagca cttcgcccac cttgatgttg ttgacgaaca cttcggcgtg gccttccacg
     1561 atggaataga cggtgtcgga ctcggtgcct tcctggatga tggtttcgcc gggcttgaag
     1621 gtctggaaac ccagcgaggt gcggtccact tccggttcga tggcggcgat gactgcggtc
     1681 tggcggcccc attcggcaat caggtaggtg ccccattcgc gggccttgtc ggtgttggcc
     1741 atggcggcgg cccagagggc gtcgcgattg taagggcgca gcaggacagg ggattcggca
     1801 tagtatttgg cgcggggcat ttcgacgccc tgggtcatgc ccaccaggtc gccggcgtcg
     1861 taatggaaca gggttttgcc gtcctgctgg tggccgatgg cgccttcttc caccacgaac
     1921 acgcagtcgc ggttggcgtc ggcagcatac agatcctcga agcattcata ggtcagcgcc
     1981 gcgcccagcg ggggaatgcc ctgcaaggcc acttccagta aatgcttggt gtggttgtcc
     2041 accttctcaa actgctcggt gtagtttgac tcgagaaaca tgcgactgcc tttccattga
     2101 ggttccgcac tgcgggggtc ttttcagtgt agacaatccg gcgggcgcag caggggaaga
     2161 tggcgcgatt cctgacccgt ggcaggtgtc gggcggaatc gcgcgggcaa gcctctgtta
     2221 caacaggggt ttgtgttctt caggcttgag acgggggccg aactgggtga ccaagcgggc
     2281 agcggccttg gtggccaggg cgccggcctg ggcgtagctg tagccgtggg tcaggccgta
     2341 caggaaggcg ccggcaaaca tgtccccggc gccattggtg tccacggcct tgaccggggt
     2401 ggcgggcacc aggtgggtgt cctggccatc gaataccagg gcgccttcgc tgcccagggt
     2461 gatggcaaag gttttggcgg tggtcttgag gcgctcggcg gcctcctcga tgctgtcggt
     2521 ctccgcccag aacagcgctt cctggcggtt gcagaacagc aggtcgacgc cgttgcccgc
     2581 catttcctgc agaccgtcgc ggaaaaattt aaccatgt
//
LOCUS       DASUEG010000361         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_461775, whole genome shotgun sequence.
ACCESSION   DASUEG010000361 DASUEG010000000
VERSION     DASUEG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461775"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFX11_06720"
     CDS             <1..502
                     /locus_tag="VFX11_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938350.1"
                     /translation="TDEVPESLNGEVNHTWQWIALVALLLWLGTLAGAAWLWRQRQAA
                     ATAQESTSADTTDRFTPAAAKAARQRFEAACAGNDARAARSALIDWYRQQFQNPQLHN
                     LHDIARQTPLPALDVALQKLDAVLYRGAREKDWKGSELQQAVTEALNRPAQNGPQSAL
                     EPLYPA"
     gene            complement(535..1602)
                     /locus_tag="VFX11_06725"
     CDS             complement(535..1602)
                     /locus_tag="VFX11_06725"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4938351.1"
                     /translation="MPNNSNRKSDHVRRPVKAVLLLATLLAFSPAHGSNRKDELQSRP
                     AIPSSKAASALLTDVSVSGKQVAVVGERGHILTSTDDGATWRQATVPVQVLLTGVYFA
                     SEQLGWAVGHEGVILGSRDGGATWEVQYANPYKEFTEEEQENFTDEQFNELPRLGAPL
                     LDVWFRNESEGFAVGAYGLLLHTSDGGKHWDDWSARLNNTDNWHLNTIGSADGNIIYV
                     AGEKGTLFRSQDGGQSWHPLQGPYEGSFFGMLVGPAPESLMVFGLQGNLFRSNDQGNS
                     WEEIKSTTENSLMGGVRLSDKDIVLVGNSGVILTSKDGGLSYTAQITKDRQAILGIAK
                     TASGKLLMVGQGGVKGGEARP"
     gene            complement(1819..3213)
                     /locus_tag="VFX11_06730"
     CDS             complement(1819..3213)
                     /locus_tag="VFX11_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX4938352.1"
                     /translation="MMLKRRIAALAAVCLVMSGTGWAKVSPEEAQQLGSTLTPYGAEK
                     AGNADGSIPAWDGGITEKRIPHTYTRRGMHHPDPYAEDKLLYKITAQNMGQYAERLPE
                     GVKGLLQTYPDSFYVPVYPSRRSASAPQWVYDNIAKNAVTAELAHDGNGFRDAFGGVP
                     FPIPKNASGQIDPVMILWNHLTRWRGIYVVRNSSDTAVQANGTYSLVTSHQEADFLYY
                     HRDGSFEKLNNRLLYYTAFVTEPARMAGNAILVHETLDSVAEPRNSWAYNAGQRRVRR
                     APTVAYDTPVAPADGLITTDDVDMFNGAADRHEWKFLGKKELLIPYNNYRLNARTLTY
                     DKLLQKGHVNPEGTRFELHRVWVIEGTLKAGTRHVYGRRTFYLDEDSWNIVAADQYDV
                     RGNLWRVSLSYLMNYYEVPVLWTALDAYHDLNARRYFVTLLDNEQDSTLEFQDASPGE
                     GHFSAQSLRRRGRR"
     gene            complement(3426..>4120)
                     /locus_tag="VFX11_06735"
     CDS             complement(3426..>4120)
                     /locus_tag="VFX11_06735"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEX4938353.1"
                     /translation="QGLRKALAEALRPALLGVYERTALRGPYEYSDAAVAKRSLHNTC
                     LSYLMTLEDDEVTQLCLRQYEQANNMTDQLAAFRGLVHSNRAEKHAVIDNFYQRWQRE
                     ALVVDQWFTAQAVAPQSDALEQVEKLLQHPAFELTNPNKVRALIGSFCNANPVNFHRR
                     DGEGYRFLADRIIELNTLNPQIASRMSTLLTRWANYDAARADKIRQQLQRIKAEPLSP
                     DVYEVITKALGQ"
BASE COUNT          793 a         1277 c         1232 g          818 t
ORIGIN      
        1 gaccgatgaa gtgccggaat cgctcaacgg cgaggtcaac cacacctggc aatggatcgc
       61 tctggtggcc ctgctgttgt ggctgggcac gctcgcgggc gcggcctggc tgtggcgcca
      121 gcggcaagca gcggcaacag cgcaggaatc cacttccgcc gacaccaccg accgtttcac
      181 ccccgccgcc gccaaagccg cccgacagcg atttgaagct gcctgcgccg gcaacgatgc
      241 ccgtgctgcc cgcagtgccc tgatcgactg gtatcgtcag cagttccaga atccgcagct
      301 gcataacctg cacgacatag cccgccagac gccgctaccg gcgctggatg tggccctgca
      361 aaaactggat gcggtgttgt atcggggagc gcgggaaaaa gactggaaag gaagcgaact
      421 gcagcaggct gtcacggaag cgctgaaccg gcccgcgcag aacggcccgc aatcggcgct
      481 ggagccgctc tatcctgcct gacagcgata gaacggctcc aaagctgtca cccgttacgg
      541 gcgggcttca ccgcccttga cgccgccctg ccccaccatc agcaacttgc ctgacgcggt
      601 cttggcgatg cccaggatgg cctggcggtc tttggtgatc tgcgcggtat agctgaggcc
      661 gccgtccttg ctggtcagga tcacaccgga attgcccacc agcacgatat ccttgtccga
      721 cagccgtaca ccacccatca gactgttctc ggtggtgctt ttgatttcct cccaggaatt
      781 gccctggtca ttgctgcgga acaggtttcc ctgcaggccg aacaccatca ggctctcggg
      841 tgccggaccc accagcatgc caaagaagga accctcatag gggccctgca acggatgcca
      901 gctctggcca ccatcctgcg aacggaacag cgtgcccttc tcgcccgcca cataaatgat
      961 attgccgtcc gccgagccga tggtgttcag gtgccagttg tcggtattgt tcaatcgcgc
     1021 cgaccagtca tcccaatgct tgccgccatc gctggtatgc agcagcaggc cataggcgcc
     1081 cacggcaaag ccttcgctct cgttacggaa ccagacgtcc agcagcggcg cgcccaggcg
     1141 gggcagttcg ttgaactgct cgtcagtgaa gttttcctgt tcttcctcgg tgaattcctt
     1201 gtagggattg gcgtactgca cttcccaggt ggcgccacca tcgcgggagc ccaggatcac
     1261 gccctcgtgc cccacggccc agcccagctg ttcgctggcg aaatagacgc cggtgagcaa
     1321 cacctgcacc ggcacggtag cctgtcgcca ggtggcgcca tcatcggtgg aggtcaggat
     1381 gtgaccccgc tcgccgacca ctgcaacctg cttgccggac acgctgacat cggtcagcag
     1441 cgcgctggca gccttggatg agggaattgc cgggcgggac tgcagttcgt ccttgcggtt
     1501 ggagccatga gccggggaaa acgccagcag ggtcgccagc agcagcaccg ccttgaccgg
     1561 gcgacgcacg tgatcagact ttctattgga attgttgggc ataaaaacgc gaaccacctc
     1621 aatgactgta tccggagaaa cccgggcggg ttctgccctg ggtttcctga tgctgttgtt
     1681 attactctgt cgttatgtgc ctgtctgcgc aggacgtccc attgtatacc cgaaagcggc
     1741 aagcctatca gccgtttgcg ccggcctgtc cggtttcaca cagggaatga ccgaatcggc
     1801 cggcacccgg gtggcggctt atcgacgtcc ccgccgccgc agcgattgcg ccgaaaagtg
     1861 gccctcgccg ggggacgcat cctggaattc cagggtgctg tcctgttcat tgtcgagcag
     1921 cgtgacgaaa tagcgccgtg cattcagatc gtgataggca tccagcgcgg tccagagcac
     1981 cggcacttcg taatagttca tcagatagga caggctgacg cgccagaggt tgccgcgcac
     2041 atcgtactga tccgccgcca cgatgttcca gctgtcctcg tccaggtaaa aggtgcgacg
     2101 gccgtacaca tggcgggtac cggctttcaa ggtaccttcg atcacccata cccggtgcag
     2161 ttcaaaacgg gtgccttcgg gattcacatg gcccttttgc agcagcttgt cataggtgag
     2221 cgtgcgggca ttcaggcggt agttgttgta gggaatcagc agttccttct tgccgagaaa
     2281 tttccactca tggcggtccg ccgcaccgtt gaacatgtca acgtcgtcgg tggtgatcag
     2341 gccatcggcc ggtgccaccg gcgtgtcgta agccaccgta ggcgcacgcc gcacccggcg
     2401 ctggcccgca ttgtaggccc aggaattgcg cggttccgcc acgctgtcca gggtttcatg
     2461 caccagtatc gcattgcccg ccatgcgcgc cggttcggtc acgaaagccg tgtagtacag
     2521 cagccggttg ttgagttttt caaaactgcc gtcgcgatgg taatacagga agtccgcttc
     2581 ctgatgactg gtgaccagac tgtaggtgcc attggcctgc accgccgtgt ccgacgaatt
     2641 gcgtaccacg tatatgccgc gccagcgcgt caggtgattc cacaggatca tcaccggatc
     2701 gatctggccg ctggcatttt tcgggatcgg aaacggcacg ccgccgaacg catccctgaa
     2761 accgttgcca tcgtgagcca gttccgccgt cacggcattc tttgcaatat tgtcatatac
     2821 ccattgcggc gccgaagcgg aacgccggct ggggtacacg ggcacgtaga aactgtcggg
     2881 atacgtctgc aacagacctt tcacaccttc cggcagacgc tcggcgtatt gccccatgtt
     2941 ctgcgcggtg atcttgtaca gtagcttgtc ttccgcatag ggatcagggt gatgcatgcc
     3001 gcggcgggta taggtgtgcg gaatccgctt ctcggtgatg ccgccatccc acgccggaat
     3061 gctgccgtca gcattgccgg ctttttcggc accgtaaggt gtaagcgtgc tgcccagctg
     3121 ctgcgcttcc tccggcgaca ctttcgccca gccagtgccc gacatgacca gacatactgc
     3181 agccagggct gcgatcctgc gtttcaacat catgggtatc ctcacgcgcc aagaaaaaac
     3241 atgcacgatt aaacagttaa gcgtgaaaaa ttcaaaacac cagccggaca aaaaagacgc
     3301 atacggtagc gaaagcgccg acgccccaca aataccgaat attgctaaga accgggaaat
     3361 ttagacaggg cccgccgcgc cgctattgca gcggcgcgac ggggattggc tgggctggtc
     3421 tgtgtttact ggcccagcgc cttggtgatc acctcgtaca catccggcga cagcggctcg
     3481 gccttgatgc gctgcagctg ctggcggatt ttgtcggcgc gggcggcgtc gtagttggcc
     3541 caacgcgtca gcagcgtgga catgcgtgag gcaatctgcg gatttagcgt gttgagttcg
     3601 atgatgcgat ctgccaggaa gcgataaccc tcgccgtcgc ggcggtgaaa gttcaccggg
     3661 ttggcgttgc agaaggaacc gatcaaagca cgcaccttgt tgggattggt gagttcgaac
     3721 gcgggatgtt gcagcagttt ttccacctgt tccagcgcgt cagactgggg cgccaccgcc
     3781 tgggcggtga accactggtc caccaccagg gcttcacgct gccagcgctg gtagaaattg
     3841 tcgatgacgg catgtttctc ggcgcggttg gaatgcacca gaccacgaaa cgctgccagc
     3901 tggtccgtca tgttgttggc ctgttcgtac tggcgcaggc agagctgcgt cacctcgtca
     3961 tcttccagcg tcatcaggta gctgagacag gtattgtgca ggctgcgctt ggccacggcc
     4021 gcgtcggaat attcataggg tccacgcagt gcagtgcgct cgtaaacgcc cagcagtgca
     4081 ggccgcagtg cctccgccag cgccttgcgc aaaccctggc
//
LOCUS       DASUEG010000362         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_535833, whole genome shotgun sequence.
ACCESSION   DASUEG010000362 DASUEG010000000
VERSION     DASUEG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535833"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="scpB"
                     /locus_tag="VFX11_06740"
     CDS             complement(<1..461)
                     /gene="scpB"
                     /locus_tag="VFX11_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX4938354.1"
                     /translation="MSLDKDKLKRILEGALFAANQPLNLEKLTALFYDEERPATELFV
                     EALQELQQDYADRAVTLKEVASGYRFQVKEDVGEWVSRLWEEKPARYSRALLETLALV
                     AYRQPITRGEIEEIRGVSVSTHITKTLLEREWIRVVGHRDVPGRPAMYGTTR"
     gene            complement(781..1542)
                     /locus_tag="VFX11_06745"
                     /pseudo
     CDS             complement(781..1542)
                     /locus_tag="VFX11_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
     gene            complement(1689..>2305)
                     /locus_tag="VFX11_06750"
     CDS             complement(1689..>2305)
                     /locus_tag="VFX11_06750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174397.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX4938355.1"
                     /translation="RNLLTKYQEQGDDEALETAHALVRDQQNITLSDKERLHGYIEGG
                     GKVILPEPQALLTPASKMPGLDGRKMSKSYNNAITLRDNEDQIAAKIRTMPTDPARVR
                     RTDAGDPEKCPVWQWHLVYSDEARKQWVQEGCRSAGIGCLDCKKPLIEAVQDELKPIR
                     IRAQDYSSNPDLVKTIVAEGCEKARDAARSTLEEVREAMGLGYF"
BASE COUNT          362 a          708 c          720 g          515 t
ORIGIN      
        1 cgggtagtac cgtacatcgc aggacggccc ggcacatcgc ggtgaccgac gacacgaatc
       61 cactcgcgct ccagcagggt tttagtgatg tgggtactga cgctgacacc acggatttcc
      121 tcgatctcac cccgggtgat cggctgacga taggccacca gcgccagggt ctccagcagg
      181 gcgcgggaat agcgcgcagg tttttcctcc cacagccggc tcacccattc acccacatct
      241 tccttcacct ggaagcggta gccgctggcc acttccttca aggtcacggc gcggtcagcg
      301 taatcctgct gcagctcctg cagggcttcc acaaacagct cggtggcagg ccgttcttcg
      361 tcatagaaca gcgcagtcag cttctccaga ttgagtggct ggttggcggc gaacagggcg
      421 ccttcgagaa tgcgtttgag tttgtcttta tcgagtgaca tcgtcggatt cttcagtctc
      481 ggcggttgct gtgtcgtctg gaatggcgtc tgacctgttg tcagcggtta cggcatcagg
      541 ccctgcacgc tcgtgttcgg atgcgccgga ttccgccgtg gattccacgt accgcagcac
      601 cgcgggatcg aacggcccgc cggcatcttc cagcagggtt tcggcggaca gctcgatgcc
      661 ctcatgatca acgctgaacg catcgggggc cggggtgcgg atctcgtagg catcatcgac
      721 gtcggtatcc gtctctgcat cggcgtagtc cgtgtcgtca cccccttcat gggctgcggc
      781 acgggcgcgt acatggatgg gcccgaaggc ttcgctctgc accagttcga tcagggattc
      841 cttcaccagt tccaggatgg cgaggaacgt gaccaccacc cccagccggc cttcttcgta
      901 ggtgaacagg ctgacaaacg gcacgaagcg gtcgttggcc agacgctcca gcacctgcgt
      961 catgcgctcg cgggtggaga gtttttcccg cgtaacctgg tggctttcga acatgtcggc
     1021 gcggtgcagc acttcctgca gtgccagcag cacttcacgc agatccacat ccggatgggt
     1081 cctgggccgt tcgtattgcg gcggctcggc ggcaaccaga tggatttccc gccccacacg
     1141 gggcatcact tcaatgtctt cggcggcctt cttgtagcgc tcgtattcct gcaggcgacg
     1201 gatcagttcg gcgcgcgggt cgagttcttc gtcttcctcg ccgggctggc gcggcagcag
     1261 catgcgggat ttgatttccc ccagcagcgc agccatcacc agatattctg ctgccagttc
     1321 caggttcagg ttcttcatca gctcgacgta ttccatgtac tgcacggtga tctgcgcgac
     1381 tggtatgtcg aggatgtcga ggttctgccg cttgatcagg tacaggagca ggtcgagcgg
     1441 gccttcaaag gcttcaagga tgacttcaag ggcgtccggc ggaatgtaca gatcctgcgg
     1501 cagcttggtg aacgcctgac cgtacaccag cgcgaacggc atttcttcct gcaccggatt
     1561 gtccggcggc gccacggcgg ccgttgcccc gttctgatgg gaagcatcct gctggccagc
     1621 gtctgccgcg ggggcttctg tcaccggcgc atcttccagc aggctgtcct ggtccgtcat
     1681 cgtcatgatc agaagtatcc caggcccatg gcctcacgca cttcttccag ggtggaacgg
     1741 gccgcgtcgc gggctttttc acagccttcc gcgacgatgg ttttcaccag atcgggattg
     1801 ctggaatagt cctgggcacg gatgcggatg ggtttgagtt cgtcctgcac cgcttcgatc
     1861 agcggctttt tgcagtcgag acagccgatg ccggcgctgc gacagccttc ctgcacccac
     1921 tgcttgcggg cttcgtcgga gtagaccagg tgccattgcc agaccgggca tttttccgga
     1981 tcgccggcgt cggtgcggcg cacccgcgcg gggtcggtgg gcatggtgcg tatcttggcg
     2041 gcgatctggt cttcgttgtc ccgcagggtg atggcgttgt tgtaggactt ggacatcttg
     2101 cgcccgtcca gacccggcat tttcgatgcg ggtgtcagca gcgcctgggg ttccggcagg
     2161 atcaccttgc cgccgccttc gatgtaacca tgcaggcgtt ccttgtcgga cagcgtgatg
     2221 ttctgctggt cgcgcaccag ggcgtgggca gtttccagcg cttcgtcgtc gccctgctcc
     2281 tggtatttgg tcagcaggtt gcggt
//
LOCUS       DASUEG010000363         9098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1836378, whole genome shotgun sequence.
ACCESSION   DASUEG010000363 DASUEG010000000
VERSION     DASUEG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836378"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFX11_06755"
     CDS             complement(<1..689)
                     /locus_tag="VFX11_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX4938356.1"
                     /translation="MFNTKTKMLRLSALSLAVAGAMGVAHTANAEGVDVSGSVGVANM
                     YLWRGNNLGGSEGGLAAVSGDLKVSAAGFYGGVWGSSGDSLWGTEYDLYVGYGAEFGG
                     VTIDLSAWNYMYPSKDDGDPTIPEYIDDFGGLSDAVLSIGFAGFTASVYDNIAGGSGY
                     EYYTLSYSFDAFSVLVGKHDNVTGVDEDQMVHVNLTYAYNDNLSFTFSQQVDQESDDD
                     LKFVASYSLPIG"
     gene            complement(968..2074)
                     /locus_tag="VFX11_06760"
     CDS             complement(968..2074)
                     /locus_tag="VFX11_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938357.1"
                     /translation="MQRMTLGLLACVLGSLAQAGDLIRVKSGETLTLDATRSEWVLDE
                     LVLEDDATLAIPASLGEVQIDAQRALFGKNSRILAPGAAGAAGQDGSAVTAAAEQCKD
                     GADGGAGTHGSAGKDGVALALTLRIAKLDSLQIDTRGGDGGKGGSGGAGGPGGDYDSC
                     NAPKGGNGGQGGDGGDGGSGGHVRVLYTLLPESGISGTLGTRISVNAPGGKGGAEGVG
                     GPGGKGADGRFVNMKTLTGNKKWMAGGKEGAAGQTGKAGRDGGKGQIVVQQDLRGRME
                     ELVQEKAQSQAVTEQGLNAVNQDVAALKVAVAGLASKELVQQQGDRVEASLTKINQAI
                     DALQKRIVQMEQQLKKPAAQPAPAAQKKPAAATP"
     gene            complement(2238..2933)
                     /gene="urtE"
                     /locus_tag="VFX11_06765"
     CDS             complement(2238..2933)
                     /gene="urtE"
                     /locus_tag="VFX11_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX4938358.1"
                     /translation="MLSIQNIDLFYGASQALKKVSFEAQKGQVACVMGRNGVGKTSLL
                     RAVAGHQSIRNGAIMWEGHNISGLAAHERAKRGIAYVPQGRMVFSQLTVLENMKTGFS
                     VLPRGEREIPEEIYELFPVLYKMLKRRGGDLSGGQQQQLAIARALVTKPRLLILDEPT
                     EGIQPSIIKDIQRVISLLRDRGEMAILLVEQYFDFARELADNYAVMDRGEVVMAGLGK
                     DMEEEKVRRYIAV"
     gene            complement(2937..3698)
                     /gene="urtD"
                     /locus_tag="VFX11_06770"
     CDS             complement(2937..3698)
                     /gene="urtD"
                     /locus_tag="VFX11_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX4938359.1"
                     /translation="MTIQFGHIPKHILYVNGVTVSFDGFKALNNLSFTVAPGELRAII
                     GPNGAGKTTMMDVITGKVKPTTGEVFFRDTIDLSQHDEAEIANLGIGRKFQKPTVIES
                     LTIFENLELALEGNRGVFRSLFHKLNGDQRKRIDEIVELVGLAEKRQWLAGALSHGQK
                     QWLEIGMLLIQGPDLLLVDEPAAGMTDHETSLIAKLLREISGERSVVVVEHDMEFIKA
                     LDCPVTVLHEGRVLAEGTLEKVQNNEEVIEVYLGR"
     gene            complement(3695..4888)
                     /gene="urtC"
                     /locus_tag="VFX11_06775"
     CDS             complement(3695..4888)
                     /gene="urtC"
                     /locus_tag="VFX11_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX4938360.1"
                     /translation="MENSRLGWLLNDVAGKRLLTVVLAAIVIIPLLNLLVPPDSVFHV
                     STYTVGLLGKYLCYALLALAVDLIWGYCGILSLGHGAFFALGGYAMGMYMMRQIGDRG
                     VYGNPLLPDFMVFLNWPELPWYWYGFDNFAFAVLMVLLAPAILAFVFGWLTFRSRVTG
                     VYLSIITQAMTYALMLAFFQNDMGFGGNNGLTDFKDILGFSLQSDNTKVALFVLSGLA
                     VILGYLLCRFVVASKLGRVLVSIRDAESRTRFVGYRVEHYKLFVFVLSAMLAGIAGAL
                     YVPQVGIINPGEFSPINSIEIIIWVAVGGRGFLYGAVIGAILVNYGKTFFTGAFPEVW
                     LFMLGALFVVSTLFFPQGVVGIVQQLKQKRAERTAFRTVAQQSGGSTESTPANDSKIL
                     QEPAP"
     gene            complement(4910..6538)
                     /gene="urtB"
                     /locus_tag="VFX11_06780"
     CDS             complement(4910..6538)
                     /gene="urtB"
                     /locus_tag="VFX11_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX4938361.1"
                     /translation="MMKIFLNIVFTVALALLFSPAMAEEDSFAQLLNELGGTNLSKKA
                     TALDQLAQLDDERVLPTLEALMNNALYQQKSDNQFVFAESVADGMKITSVVSGAVLGE
                     TVSSRDLRKIITNNSLRSRARNLVAATRLMNKKAEVREAAALELIKSPNIEFLQPLDN
                     AIAREKVPAIQQALQVARGAITLDHPDEIERLKAVQAMDGFKHKQVITLLEQRLQKNA
                     DGSFVEPSELVRATAQDVVDGLKQSEFFYKMIEDTFFGLSLGSVLLLAAVGLAITFGV
                     MGVINMAHGEMIMLGAYTTYVVQLLMPSNIGASLIVAVPAAFLVAGLMGVIIERGVIR
                     FLYGRPLETLLATFGISLILQQLVRTIFSPLNRSVITPEWMSGSLEINGFLSLTYNRL
                     YIVLFALAVLFGLMLLLKKSFFGLQMRAVTQNRAMASSMGIRTGWVDAMTFGLGSGIA
                     GIAGVALSQLTNVGPNLGQAYIIDSFMVVVFGGVGNLWGTFVAAMGLGVANKFIEPLS
                     GAVLAKILMLVFIILFIQRRPRGLFALKGRFVEG"
     gene            complement(6605..7909)
                     /gene="urtA"
                     /locus_tag="VFX11_06785"
     CDS             complement(6605..7909)
                     /gene="urtA"
                     /locus_tag="VFX11_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX4938362.1"
                     /translation="MKISKSTWLKTVQGAAVAASLSFVSGMAMAADTIKVGVLHSLSG
                     TMAISETTLKDTVLMLVEEQNKKGGLLGKKLEAVVVDPASNWPLFAEKAKELLEKEKV
                     DVMFGCWTSVSRKSVLPVVEGLNGLLFYPVQYEGEESSKNVFYTGAAPNQQAIPAVDY
                     LMKDVGVKRWVLAGTDYVYPRTTNKILEAYLKSKGVAAEDIMINYTPFGHSDWQTIVS
                     DVKKFGSAGKKTAVVSTINGDANVPFYKELANQGVSAEDIPVVAFSVGEEELSGFDTK
                     PLVGHLAAWNYFMSSESPENDAFIKAWHAYIKNTKRVTNDPMEATYIGFKLWVKAVEK
                     VKTTKVDDVSKAIIGLEVPNLTGGTAKMLPNHHLTKPVLIGEIQEDGQFQTVWQTESE
                     VPGDAWTDFLPESKKIVADWADPKIMCGNYNTETKTCSGQNY"
     gene            complement(8337..>9098)
                     /locus_tag="VFX11_06790"
     CDS             complement(8337..>9098)
                     /locus_tag="VFX11_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX4938363.1"
                     /translation="PGVSDDCLTQWSFNSLGVYTQPGVVESIVKKSTEMNLQLQEISD
                     TNPEKDPDLYIFGLGGTFKGYYPGYSGQTFDPDNQFTWLVLKGHSSNKGVVELRSADP
                     RDTPIINFRYFGDPALGVGPSGDHLKDLDAVVKGIRLARLASTGASVTEALGSGGIYE
                     EVWPGPNVTTDDQLRQYAMNESWGHHASCTAKIGSALDSMAVLDSRFRVRGVDRLRVV
                     DASAFPRIPGFFPVVAIYMMSEKAADAITADAPLY"
BASE COUNT         1940 a         2810 c         2464 g         1884 t
ORIGIN      
        1 ccgatgggca ggctgtagga agcgacaaat ttcaggtcgt catcagattc ctggtctacc
       61 tgctggctga aggtgaagga caggttgtcg ttgtaggcgt aagtcaggtt aacgtgcacc
      121 atctgatctt catcaacacc cgttacgttg tcgtgcttgc ccaccagcac gctgaacgcg
      181 tcgaagctgt aagacagagt gtaatattca taaccggacc cgccggcaat gttgtcatac
      241 acagatgcag tgaagccggc aaaaccgata gacaacacgg catcgctcaa gccgccgaag
      301 tcgtcaatgt attctggaat ggtcggatcg ccatcatcct tgctcgggta catgtagttc
      361 catgcactca ggtcgatggt cacaccaccg aattctgcac cgtagcccac gtaaagatcg
      421 tactcagtac cccacaagga gtcaccgctg ctgccccaga cgccgccgta gaagcccgct
      481 gcacttacct tcaaatcacc ggaaacagcc gcaaggccac cttccgaacc acccaaatta
      541 ttaccacgcc acaaatacat attggccacg cccacggaac cggacacgtc aacgccttca
      601 gcattggcgg tgtgagcaac gcccattgca ccggcaacag ccagggacag agcgctaaga
      661 cgcaacattt tggttttagt gttgaacata agaaataacc cccagaggtt agacgatata
      721 aggtttgcac cttggttagc gaactcgctg atgatttagc acagagcgtg ccagcgtcgc
      781 ggaggcctcg aatactgcgg cttttgcgcg cattttccgt ttgtgaattt cacacaaatg
      841 acacgccggc acaagcatgg tgcgcccctt tttggtgcgt gtgctttatt ggtgcgaaag
      901 tgacaaggtg atttggcccg ccggattatc atcacagcgg gccgggatgc gaccgtgcga
      961 acgacgatca gggtgtggca gcggcgggtt ttttctgcgc ggcgggagca ggctgtgcag
     1021 ccggtttctt caactgctgt tccatctgaa cgatgcgctt ctgcagagca tcgatagcct
     1081 gattgatctt cgtcaaggat gcctccaccc gatccccctg ctgttgcacc aattccttcg
     1141 acgccagccc ggccactgcc actttcagtg cggccacgtc ctggttgacg gcattcagtc
     1201 cctgctccgt taccgcctga ctctgggctt tttcctgcac cagctcttcc atgcggcccc
     1261 gcaaatcctg ctgcaccact atctggccct tgccgccatc gcggcccgcc ttgccggtct
     1321 gtccggcggc gccttccttg ccaccggcca tccatttttt attgccggtg agggttttca
     1381 tgttaacgaa gcgtccgtcc gctcctttgc ctcccgggcc acccacacct tccgcgcccc
     1441 ctttgccacc gggcgcattg acgctgatgc gggtacccag cgtgccgctg atgccgctct
     1501 cgggcagcag ggtatacagc acgcggacat ggccgccgct gccaccatcg ccgccgtcgc
     1561 caccctgacc gccattgcca cccttgggag cattgcagga atcgtaatcg ccaccaggac
     1621 caccggcacc gccactgccg cctttgccgc cgtccccgcc ccgggtgtcg atctgcaggg
     1681 aatccagctt cgcgattcgc agcgtcaacg ccagagccac accgtctttt ccagcactgc
     1741 cgtgagtacc tgcgccaccg tcagcgccgt ccttgcactg ttctgcggca gcggtgactg
     1801 cgctgccgtc ctggcccgcc gcaccagccg cgccaggcgc cagaatacgg ctgttcttgc
     1861 cgaacagtgc ccgctgcgca tcgatctgga cttcgcccag ggatgcggga atggccaggg
     1921 tggcatcgtc ttccagcacc agttcgtcca gcacccattc ggaacgggtt gcgtccaggg
     1981 tcagggtttc gccggatttc acccggatca gatcgccggc ctgcgccagg ctaccaagca
     2041 cgcaggccag caggcccagg gtcatacgtt gcatagggat ttcccttctg aagaaagtgg
     2101 agggtggaga catgtttcaa tggtagccgc ttgcaccgtt ctggaacagc gctggccgga
     2161 gcggagaaac cgcaggcaaa aaaaaccgct gcgccggaca gaggtccgcc gacgcagcgg
     2221 ggaaagaaac ccgctggcta cactgcgatg tagcgccgga ccttctcttc ttccatatcc
     2281 ttgcccagcc cggccatcac tacttcgccc cggtccatca cggcatagtt gtcggccagc
     2341 tcgcgggcga aatcgaagta ctgttccacc agcaggattg ccatttcacc gcgatcccgc
     2401 agcagtgaaa tcacgcgctg gatatccttg ataatcgacg gctggatgcc ctcggtgggc
     2461 tcgtccagaa tcagcaggcg cggtttggtg accagcgcac gggcaatcgc cagttgttgc
     2521 tgctgaccgc cggacaaatc accgccacgc cgtttcagca ttttgtagag cacgggaaac
     2581 agctcataaa tctcttccgg aatttcacgc tcgccacggg gcaacacgga gaaaccggtt
     2641 ttcatgtttt ccagcacggt gagctgcgag aacaccatgc gcccttgtgg tacataggcg
     2701 atgccgcgct tggcgcgttc gtgggcggcc aggccggaga tgttatgacc ttcccacata
     2761 atggcgccgt tgcgaatgga ctggtggccg gccaccgcac gcagcagcga ggtttttccc
     2821 acgccgttac gacccatcac gcaggccacc tggcctttct gtgcctcgaa gctgactttc
     2881 ttcaacgcct ggctggcgcc ataaaacagg tcgatattct gaatgctcag cattggtcag
     2941 cgccccaggt acacttcgat gacttcttca ttgttctgca ctttttccag cgtgccttcc
     3001 gccagcacgc ggccttcatg cagaacggtg acggggcaat ccagcgcctt gatgaattcc
     3061 atgtcgtgct ccaccaccac cacagagcgc tcgccgctga tttcccgcag cagtttcgcg
     3121 ataagcgacg tttcatgatc ggtcatgcca gcggccggct cgtccaccag caaaagatcg
     3181 gggccctgga tcagcagcat gccgatttcc agccactgct tctggccgtg cgacagcgcg
     3241 ccggccagcc actgccgctt ttctgccagt cccaccagtt cgacaatttc atcgatgcgc
     3301 ttgcgctgat cgccattgag cttatggaac agtgaacgga acacgcctcg attgccttcc
     3361 agcgccagct ccaggttttc gaaaatggtc aggctttcga tcacggtcgg tttctggaat
     3421 ttacggccga tacccaggtt ggcgatttcg gcttcgtcgt gctgcgacag gtcgatggtg
     3481 tcccgaaaaa acacttcgcc ggtggtcggt ttcaccttgc cggtgatcac gtccatcatg
     3541 gtggtcttgc ctgcgccatt cggcccgatg atggcgcgca gctcgcccgg cgccacagtg
     3601 aacgacaggt tgttcagcgc cttgaagccg tcaaaggaaa ccgtcacacc gttcacgtac
     3661 aggatatgct tgggaatatg gccaaactgg atggtcatgg cgcgggctcc tgcaggatct
     3721 tgctgtcgtt cgccggcgtg ctttcagtgc tgccgccgga ctgctgtgcc actgtccgga
     3781 aggccgtgcg ctccgcccgc ttctgcttga gctgctgcac gatgcccacc accccttgcg
     3841 ggaaaaacag ggtagacacc acgaacagcg cgccgagcat gaacagccag acttccggaa
     3901 acgcgcccgt gaaaaaggtt ttgccgtagt tcaccagaat cgcaccaatc actgcgccat
     3961 acaggaaacc gcggccgccc accgccaccc agatgatgat ctcgatggag ttgattggcg
     4021 aaaattcacc gggattgatg atgcccacct gcggcacata gagcgcaccg gcaatacccg
     4081 ccagcatggc gctgagcaca aacacgaaca gcttgtaatg ctcgacgcgg taaccgacga
     4141 aacgggtgcg gctttccgca tcgcgaatcg acaccagcac gcggccgagt ttcgacgcca
     4201 ccacaaaccg gcacagcagg taacccagaa tcaccgccag tcctgacagc acaaacagcg
     4261 ccaccttggt gttatcggac tgcaggctga aaccgagaat atccttgaaa tccgtcagac
     4321 cgttattgcc gccgaagccc atgtcattct ggaaaaacgc cagcatcagc gcataggtca
     4381 tcgcctgcgt gatgatggag agataaacgc cggtaacgcg ggaacggaac gtgagccagc
     4441 cgaacacaaa ggcgaggatc gccggcgcca gcagcaccat cagcacggca aaggcgaaat
     4501 tatcgaaacc gtaccagtac cacggcagtt ccggccagtt cagaaacacc atgaagtccg
     4561 gcagcagcgg attgccatac acaccgcgat cgccgatctg ccgcatcatg tacataccca
     4621 tggcatatcc gcccagcgcg aagaaagcgc cgtggcccag actgagaata ccgcagtagc
     4681 cccagatcag atccactgcc agcgccagca atgcgtagca caggtatttg cccagcaggc
     4741 ccacggtgta agtggacaca tgaaaaacgg aatccggcgg caccagcaga ttgagcagcg
     4801 gaatgatgac gatcgccgcc agcaccaccg tcagcaggcg cttgccagcc acatcgttca
     4861 gcaaccagcc gagtcgggaa ttttccaaca cttttgcgtc attcacggct caaccctcca
     4921 caaaccggcc tttcagcgcg aacaatccgc gcggccgccg ttgaatgaac agaatgatga
     4981 ataccagcat caggatcttc gccaacaccg cgcctgaaag cggctcgatg aatttgttcg
     5041 ccacccccag ccccatcgcc gcgacaaaag tgccccacaa attaccgaca ccgccaaaca
     5101 ccaccaccat gaacgaatcg atgatgtagg cctgacccag attcggaccc acattggtga
     5161 gctggctcag cgcaacaccg gcgatacccg caatgccgga gccaaggccg aaagtcatcg
     5221 catccaccca gccagtgcgg atgcccatgg aactggccat ggcgcggttc tgcgttaccg
     5281 cgcgcatctg caggccgaag aaacttttct tcaacagaag catcaggcca aacagcaccg
     5341 ccagcgcgaa caacacgata taaagccggt tataggtcag cgacaggaaa ccgttgatct
     5401 ccagcgagcc actcatccat tccggcgtga tcaccgagcg attgagcggg gagaagattg
     5461 tgcgcaccag ctgctgcagg atcaggctga tgccgaaggt ggccagcagg gtttccagcg
     5521 gccggccata caggaaacgg atgacgccac gctcgatgat cacgcccatc aggcccgcca
     5581 ccagaaacgc cgccggcacc gccacaatca gcgaagcacc gatattgctg ggcatcagca
     5641 gctgcaccac gtaggtggtg tacgcaccca gcatgatcat ctcgccgtgc gccatgttga
     5701 tgacgcccat gacgccaaag gtgatcgcca ggccgaccgc cgccagcagc agcaccgagc
     5761 ccaggctcaa accaaagaac gtgtcttcaa tcatcttata gaaaaattct gattgcttga
     5821 gcccgtccac cacatcctgc gcagtggccc gcaccagctc actgggctca acgaaactgc
     5881 cgtccgcatt tttctgcagc cgctgttcca gcagggtaat cacctgcttg tgtttgaaac
     5941 catccatggc ctgcaccgct ttgagccgct cgatttcgtc aggatgatcc agggtgatgg
     6001 cgccacgcgc cacctgcagc gcctgctgga tggccggcac tttttccctg gcaatggcgt
     6061 tatccagggg ttgcaggaat tcgatgttgg gcgatttgat cagttccagc gccgcagctt
     6121 cacgcacttc ggccttcttg ttcatcagcc gggtggccgc caccagattg cgggcgcggg
     6181 accgcaggct gttgttggtg atgatcttgc gcaggtcacg gctggataca gtttcaccca
     6241 gcacggcgcc gctcaccacc gaggtaattt tcatgccgtc cgccactgat tcggcaaaga
     6301 caaactggtt gtcggatttc tgttgataca gcgcgttgtt catcagcgct tccagggttg
     6361 gcagcacgcg ttcatcgtcc agctgcgcca gctggtccag tgccgtggct tttttgctta
     6421 aattggtacc gcccagctca ttgagcagtt gcgcgaaact gtcttcctcg gccatggccg
     6481 gtgaaaacaa cagagcgagc gcaaccgtga acacaatgtt cagaaaaatt ttcatcatga
     6541 tcgccagtca tcgattggaa atgggggact gctccggcac aaggccggag caggtgttgc
     6601 gcgatcaata gttctgaccg gaacaggttt tggtttcggt gttgtagtta ccgcacatga
     6661 ttttcggatc agcccagtcg gccacgatct tcttgctttc cggcaggaaa tcggtccagg
     6721 catcacccgg tacttcggat tcggtctgcc acacggtctg gaactggccg tcttcctgga
     6781 tttcgccgat caaaaccggc ttggtcaggt ggtggttcgg cagcattttg gcagtaccgc
     6841 cagtcaggtt cggcacttcc agaccgatga tggccttgga aacgtcatcc actttggtgg
     6901 tcttcacttt ttccaccgct ttcacccaca gcttgaaacc gatataggta gcttccatcg
     6961 gatcgttggt gacgcgcttg gtgttcttga tgtacgcatg ccaggctttg atgaaggcat
     7021 cgttctcggg cgattcggaa ctcatgaagt agttccatgc cgccaggtga cccacgagcg
     7081 gtttggtgtc gaagccggac agctcttctt cacctaccga gaaggcaacc accggaatat
     7141 cttcggcaga gacgccctgg ttggccagtt ctttatagaa gggaacgttg gcgtcaccat
     7201 tgatggtgga cacaactgcg gttttcttgc cggcggagcc gaatttcttc acatcggaca
     7261 caatggtctg ccagtcggag tgaccgaacg gcgtgtagtt gatcatgatg tcttctgcag
     7321 caacgccttt gctcttcaga tacgcttcca gaatcttgtt ggtggtgcgg ggataaacgt
     7381 aatcggttcc cgccagcacc caacgcttca cacccacgtc tttcatcagg taatcaacag
     7441 cggggatggc ctgctggttg ggcgcggcac cggtgtaaaa cacgttcttg gaagattctt
     7501 caccttcata ctgcaccggg tagaacagca ggccgttgag gccttccact accggcagaa
     7561 cagatttgcg cgatacggat gtccagcagc cgaacatcac atccactttt tccttttcca
     7621 gcagttcttt ggctttttcc gcaaacagtg gccagttgga tgcaggatca accacgacgg
     7681 cttcgagctt cttgcccagc agaccgcctt tcttgttttg ctcttccacc agcatcagca
     7741 cggtgtcttt cagcgtggtt tcgctgattg ccatggtgcc agacaatgaa tgcagcacac
     7801 cgaccttgat ggtgtcggcg gccattgcca tgccggatac gaaagagagc gatgctgcca
     7861 ccgcagcgcc ctggacggtc ttcagccagg tggatttgct tattttcata gaactccccc
     7921 aatgggtttt tttggtgtgt gaaatgctca gggggaaagg cattttctgt gccatcgcgt
     7981 atttttgatg ctgtacgggc ctgtaacact gttgcaagcc ggtagaagcg gccggaacgg
     8041 caccataatg gagcgcttca aatcatcgca tcgcatttcg tgtgcacaga tcaccagaat
     8101 aggttcaggc aggggcgaga aatgagcaaa attaaatcag ctgtgcggta ttgcggtaaa
     8161 aaagaggttc aacaccggac ttctgcgcct ccagccgcct tgttttgggg cgtcgatgtc
     8221 cgttttacgg ggcacgcagg gttgtcagag caccaagtta acgacgctag aagaggtccg
     8281 tcagcaggcg acgacagagc atcagctccg aaccgtcgcc cgccattgtc acgcgctcag
     8341 tacaggggcg catcggccgt gatggcatcg gcggctttct cgctcatcat gtaaatggcc
     8401 accacgggga aaaatccggg gatgcgcggg aatgcactgg catccacaac ccgcaaccgg
     8461 tccacgccgc ggacacgaaa acgactgtcc agcacagcca tggagtccag cgccgatccg
     8521 attttggccg tgcaggacgc atgatgcccc caggattcgt tcatcgcata ctggcgcagc
     8581 tggtcgtccg tggtgacatt cggcccgggc cacacctcct cgtagatgcc gccactacca
     8641 agtgcctcgg tcacactcgc gccggtggag gccagacgcg ccagacgaat ccctttcacc
     8701 accgcatcca gatctttcag gtgatcacca ctgggcccga cacccagcgc cggatcgcca
     8761 aaatatctga agttgatgat gggcgtgtcg cggggatcgg cactgcgcag ctccaccaca
     8821 cccttgttgc tgctatgccc tttcaacacc agccaggtga actggttatc cggatcaaag
     8881 gtctgcccgc tgtaacccgg gtagtagccc ttgaacgtgc cgcccagacc gaagatataa
     8941 agatccggat ccttttcagg gttggtgtcg ctgatttcct gcagctgcag gttcatttcg
     9001 gtggatttct tgacgatgct ttccaccaca ccgggctgcg tgtatacacc gaggctgttg
     9061 aacgaccact gtgtcaggca gtcatccgac acccctgg
//
LOCUS       DASUEG010000364         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2161960, whole genome shotgun sequence.
ACCESSION   DASUEG010000364 DASUEG010000000
VERSION     DASUEG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161960"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1976
                     /locus_tag="VFX11_06795"
     CDS             195..1976
                     /locus_tag="VFX11_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4938364.1"
                     /translation="MESDTQDRTRPATPAVQDNTDRQSSDAVSRLRSLILGEDYETAI
                     RQARDADETRRVAAVVAEAIRLRSQQDKSVQNALAPAIDDVLDASIRRDPVKLARVIS
                     PIIGPSIRAAVRTALADMVESLNRILERSISPQSWWWRVQAWRAGMPFGQFVLLRTLQ
                     FRVEQVLLIHRDSGILLQAETAHGVEIHDPQLVSAMLTAIKDFVSDSFRSGNDAADLK
                     RVRFGDRLLLIEVGPQAVMAAVVLGDPPPGLATQLSTTLEQFHRIFAQPLADFSGDRA
                     PFQPATGLLQDCITEQVRADRASAKPWLALSAIALVVIVAVLFGWRQWRIDQLRADIV
                     QQLQASPDYVVLGSEGDGRHLRVNLLSRFGASSPQAVQALAQQSGIELELHMAPVAMN
                     SEDLLLPYLERRYGLGPQVSVQLQNGVLQIAGPLSAQQLDQIRADSWVRGLVRTLDSA
                     RVNIIPPPDQRALDQQHWRELVDTIQHERFYFEEDSPLLDAREGGKAARMLEQLRELT
                     ALAKKLELQHLQILVQGFADSTGTDARNSRLSRDRALELRDFFIAHGVDPGLLTAMGV
                     GSLGASTLTAQQKRFAMLQVIYVMPPD"
     gene            2011..2505
                     /locus_tag="VFX11_06800"
     CDS             2011..2505
                     /locus_tag="VFX11_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HEX4938365.1"
                     /translation="MVVKKICMMGAFAVGKTSLVRRFVHSIFSETYQTTIGVMISKKK
                     ITLAEQEFELIIWDLEGRDEFTDFRSSYLRGVSGVIFVVDVTRPQTLEVARQLREEVH
                     HHIGAVPALILLNKADLAEQWRLDPATLELLEQEGCTLLHTSAKTGANVEEGFLALLR
                     SMTV"
     gene            2506..>3872
                     /locus_tag="VFX11_06805"
     CDS             2506..>3872
                     /locus_tag="VFX11_06805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX4938366.1"
                     /translation="MTLFSQLLSDMGMLVLECKPGPVFVALNRIPDWFQQIHSAPLGA
                     DAMLDVVTIFPFIAHFIEDADVFWRNPVEARLGSGVWTELDEQGKQHQLEAYALLYQN
                     TRILLLVDLSASFDARHQVYQRAREIALEQERLVMSLRRQQRKLQLQLQGLLTRDNGL
                     ASIDERIRQHASAVMVCRPDGSVELLNQALMDIYPVAGNNGTRAEYSLLKQWVSEAEA
                     LYPEIHRVIETGASWEGEFESSAGSDKKWVRLLIGPVLDQEQRVTHLVCVANDISGLR
                     EPALELERVTDFDFTTQLPNRRHFWRVLTRAIDECRASGEKLVLLYLDVDHFKRINDS
                     LDHQAGDLLLGTLSSRLRRNIKKADFIAHLGGDEFALMLRTSQRTQDLLPVAERLLGV
                     VRQPLVVDGHNVSITASIGIACFPEHGNDAVGLMKRADLAMYHAKQRGRDQLLMFSRS
                     LDEAF"
BASE COUNT          735 a         1156 c         1229 g          752 t
ORIGIN      
        1 gcaacaactg atggacatgg ccgccaccct gcaggaatcc atcagccacc ggtcgcagga
       61 gctgctggag cgcctcaacc agacccaggt ggaattgcgc gacagcaagg ccgaccgcaa
      121 aatactggcc aatctgctgg atcgcatggc gactgaactg aatcaggaca gctgaaggct
      181 gaaggtgtcc ctgcatggaa agcgacacac aggatcgcac ccgcccggca acgcctgccg
      241 tccaggacaa tactgaccgc cagagcagcg acgcggtgtc acgtctgcgc tccctgatac
      301 tgggggaaga ttacgaaacc gcgatccgtc aggcccgtga cgccgatgaa acccggcgcg
      361 tggccgctgt cgtcgcagaa gccattcgtc tgcgcagcca gcaggacaaa agcgtgcaga
      421 acgcactggc accggccatc gacgatgtgc tcgatgcgtc catccgccgc gatccggtca
      481 aactcgcccg cgtcatttcc ccgatcatcg gtccgtccat ccgtgccgct gtgcgtaccg
      541 cgctggcgga catggtggaa tccctcaatc gcatactgga acgcagcatc agcccgcaat
      601 cctggtggtg gcgggtgcag gcctggcgcg ccggcatgcc cttcggccag ttcgtgttat
      661 tgcgtaccct gcagttccgg gtggaacagg tgctgctgat acatcgcgac agcggcatcc
      721 tgctgcaggc ggagacggcg cacggtgttg aaatccatga cccgcagctg gtctcggcga
      781 tgctcacggc gatcaaggat tttgtcagcg attcgtttcg ctccggcaac gatgcggccg
      841 atctcaaacg cgtgcgtttt ggcgaccggc tgctgctgat cgaagttggc ccccaggcgg
      901 tgatggccgc ggtagtgctg ggtgatccgc cgccgggcct ggcaacgcag ctgtccacca
      961 cgttggaaca gttccaccgc atatttgcgc agccgctggc ggacttcagc ggcgaccgcg
     1021 ctccattcca gccggcaacc gggctgctgc aggattgcat caccgaacag gtccgcgccg
     1081 atcgcgcgtc ggccaaaccc tggctggcgc tgtcggccat tgcgctggtg gtaatcgttg
     1141 ccgtgctgtt cggctggcgc cagtggcgca tcgaccagtt gcgggctgac atcgtgcagc
     1201 agctgcaggc gtctcccgat tatgtggtgc tgggcagcga gggtgatggc cggcatttgc
     1261 gggttaacct gctgtcgcgt tttggcgcat cgtcaccgca ggcagtacag gctctggcgc
     1321 agcaatccgg catcgaactt gaactgcaca tggcgccggt cgcgatgaat tcggaagatt
     1381 tattgctgcc ctatctggag cggcgttacg gtctgggtcc gcaggtttcc gtgcaattgc
     1441 agaatggtgt gctgcagatt gcgggtccgc tcagtgcgca gcagctggac cagatccgcg
     1501 ctgacagctg ggtgcgggga ttggtgcgca cactggattc cgcccgggtg aacatcattc
     1561 cgccgccgga tcagcgcgcg ctggaccagc agcactggcg cgagttggtg gataccatcc
     1621 agcacgaacg cttttatttc gaagaggatt cgccgctgct ggacgcgcgt gaaggcggca
     1681 aagcggcccg catgctggaa cagctgcgtg agctgaccgc actggcgaaa aagctcgaac
     1741 tgcaacatct gcagatcctg gttcaggggt ttgcggacag caccggcacc gatgcacgca
     1801 actcgcgcct gagtcgcgac cgggcactgg agctgcgcga ttttttcatc gcccacggtg
     1861 ttgatccggg gctgttgacc gccatgggtg tgggcagcct gggggccagc acactcactg
     1921 cgcagcagaa gcgttttgcc atgctgcagg tgatatacgt aatgccaccc gattgaacga
     1981 aaggttgatg cgggcaggag tgccgctctg atggtcgtca agaagatctg catgatgggg
     2041 gctttcgcgg tcggcaagac cagcctggtg cgtcgcttcg tgcacagcat attttccgaa
     2101 acctaccaga ccaccatcgg cgtgatgatc agcaagaaaa agatcaccct ggcagagcag
     2161 gaattcgaac tcatcatctg ggacctggaa gggcgtgacg aatttaccga ttttcgcagc
     2221 agctatctgc gcggcgtatc cggcgttatt ttcgtggtgg atgtgacccg gccccaaacc
     2281 ctggaagttg cgcgccagct gcgtgaggaa gtacaccatc acatcggcgc ggttccggcc
     2341 ctgatcctgc tgaacaaggc tgatctggcg gagcagtggc gcctggatcc tgccacgctg
     2401 gagctgctgg aacaggaggg ctgcaccttg ctgcacacca gcgccaaaac cggcgccaac
     2461 gtcgaggagg gttttctggc gctgctgcgc tcgatgacgg tctgaatgac gctattttcc
     2521 caactgcttt ccgatatggg catgctggtg ctggagtgca aacctggccc ggtgttcgtc
     2581 gcgctgaacc gcattcccga ctggttccag caaattcact cggcgccgct gggtgcggat
     2641 gcgatgctgg acgttgtcac catttttccc ttcattgccc atttcatcga ggatgccgac
     2701 gttttctggc gcaatccggt tgaggcacgt ctggggtctg gtgtgtggac cgagctggac
     2761 gagcagggca agcagcatca actggaagcc tacgcgctgc tgtaccagaa cacccgcatt
     2821 ctgctgctgg tggatctcag tgccagtttc gacgcgcgcc atcaggtgta ccagcgggcg
     2881 cgggagatcg cactggagca ggagcgactg gtcatgtcgc tgcggcgcca gcagcgcaaa
     2941 ctgcagttgc aactgcaggg cctgctgacg cgggacaatg ggctcgccag cattgacgag
     3001 cgcattcgtc agcacgcttc cgccgtcatg gtgtgccggc ctgatggctc ggtggaactg
     3061 ctcaaccagg cgctgatgga catatacccc gtcgccggca acaacggcac ccgtgccgag
     3121 tattcgttgc tgaaacagtg ggtgagcgag gcggaggcgc tgtacccgga aattcaccgc
     3181 gtcattgaaa ccggcgcgag ttgggaaggc gaattcgaat ccagtgccgg cagtgataaa
     3241 aaatgggtgc gcctgctgat cgggccggtg ctggatcagg aacagcgtgt cacccacctg
     3301 gtctgtgtgg cgaatgacat cagcggcctg cgggagcctg ccctcgagct ggaacgtgtt
     3361 accgatttcg acttcacgac gcaactgccc aaccggcgtc atttctggcg cgtgctgacc
     3421 cgcgccatcg acgaatgccg tgccagtggc gaaaagctgg tgttgctgta tctcgacgtg
     3481 gatcatttca aacgcatcaa cgacagcctc gaccatcagg ccggcgacct gctgctggga
     3541 accctgtcgt cgcggctgcg gcgcaatatc aaaaaagccg atttcatcgc ccacctgggc
     3601 ggtgatgaat ttgcgctgat gctgcgaacc tcacagcgca cacaggattt gttaccggtg
     3661 gcggagcgcc tgctgggcgt ggtgcggcag ccgctggtgg tagacggcca caacgtcagc
     3721 atcactgcca gtatcggcat cgcctgtttt cccgagcacg gcaacgacgc cgtgggtctg
     3781 atgaagcgcg ctgatctggc catgtatcac gccaagcagc gcggccgcga tcagctcctg
     3841 atgttcagcc gcagtctgga cgaagccttt tt
//
LOCUS       DASUEG010000365         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_945816, whole genome shotgun sequence.
ACCESSION   DASUEG010000365 DASUEG010000000
VERSION     DASUEG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945816"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VFX11_06810"
     CDS             <1..1372
                     /locus_tag="VFX11_06810"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4938367.1"
                     /translation="TDFDILEKANSAGGTWRENTYPGLACDVPGYLYTYSFEPNPECS
                     HRYARGDEIQAYFLRVFNQYQLHRHTRFGQEILSARHADGVWHITTRTGDQWQADILI
                     SAVGVLHHPNLPDIPGLDSFQGTAFHSARWNHAAPVDGKRVAVIGTGSTAVQIVSGLV
                     DRVAELKLFQRTAQWVLPAFDTEYSDWSKVILRRMPGLTKWLHNRYSDVYHGILSGGF
                     GRERVVFINAFRMMAERHLNSVRDPELRRRLTPDYRFGCKRFILSDRFYDAMQQPQTQ
                     LVTDRIDRIEPGGIRTSDGTLHEVDVIVLATGFKAQNYVRPIQLTGTSGISLEQAWAD
                     GVKAYKTVAIPGFPNFFMLQGPHSPIGNFSLIKIAEMQCEYLMQLIQRICSGDIRTLA
                     PKSAATDDYNRALREAAKNTIWMDPGCHSWYIDTRGNLTIWPWSIGRFDAIMKAPELA
                     DYEIVR"
     gene            complement(1433..1744)
                     /locus_tag="VFX11_06815"
     CDS             complement(1433..1744)
                     /locus_tag="VFX11_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938368.1"
                     /translation="MNKLLWSLCLPGALLLSGCALNDARHEQCGRPDFLYCLGAKPEQ
                     CDQMFDKANVECNAKMADNSMFANMPDNMKQNYTNRCIVDSLVAQSGKEEDKVKGCIR
                     W"
     gene            complement(1771..>2280)
                     /locus_tag="VFX11_06820"
     CDS             complement(1771..>2280)
                     /locus_tag="VFX11_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4938369.1"
                     /translation="PEWQEKVRAEARIFNKPALSFDDLAEATMTELCMKEALRMYPPL
                     PSMPRMTTRAVEFSGYLIPANTLVSVSPVHTHYMEEIWTDPDTFDPLRFAPPREEHKQ
                     HPFAWVPFGGGAHMCIGQHFADLQVKAIMHQLVLRFRWSVPRDYEIPYQLVPIAKPKD
                     DLPIRLERL"
BASE COUNT          471 a          719 c          640 g          450 t
ORIGIN      
        1 caccgatttc gacattctgg aaaaggccaa cagcgccggc ggcacctggc gcgaaaacac
       61 ctatcccggc ctcgcctgcg atgtgcccgg ctacctttac acctactcct tcgaacccaa
      121 tcccgaatgc tcgcaccgct atgcccgggg tgacgaaatc caggcgtatt tcctgcgggt
      181 gttcaaccaa tatcagttac accgccatac gcgttttggg caggagatcc tgtccgcgcg
      241 ccatgccgat ggcgtctggc acatcaccac ccgcaccggc gaccagtggc aggccgacat
      301 cctgatttcc gccgtgggcg tgctgcacca ccccaacctt cccgacattc cagggctgga
      361 cagtttccag ggcacggctt ttcacagcgc acgctggaat catgcggcgc cggtggacgg
      421 caagcgggtg gcagtgattg gcaccggctc cacggccgtg cagattgtca gcggactggt
      481 ggaccgggtg gctgaactga aactgttcca gcgcacggcg cagtgggtgc tgcccgcctt
      541 cgacaccgaa tattcggact ggtcgaaggt aatcctgcgc cgcatgccgg gattgacgaa
      601 atggttgcac aaccgctatt ccgatgtgta ccacggcatt ttgagtggcg gctttggtcg
      661 cgaacgcgtt gtgttcatca acgcctttcg catgatggcg gaacggcatc ttaacagcgt
      721 gcgcgatccg gagttgcggc gccggctcac gcccgactac cgcttcggct gcaaacgctt
      781 cattctgtcg gaccgctttt acgacgccat gcagcaaccg caaacgcaac tggtgaccga
      841 ccgcatcgac cgcatcgaac ccggcggcat ccgcaccagc gacggcaccc tgcatgaagt
      901 ggatgtgatc gtgctggcca ccggcttcaa ggcacagaat tatgtgcggc caatccagct
      961 gacgggtacc agcggcatca gcctggagca ggcctgggcc gacggcgtca aggcatacaa
     1021 aaccgtggcc attcccggct ttcccaattt cttcatgctg caggggcccc acagccccat
     1081 cggcaatttt tcgctgatca agattgccga gatgcagtgc gaatatctga tgcagctcat
     1141 ccagcgcatc tgcagcggcg acatccggac gctggccccg aaaagcgccg ccacggatga
     1201 ctacaaccgc gcactgcgcg aagctgcaaa aaatacaatc tggatggacc cgggctgcca
     1261 tagctggtac atcgatacgc gcggcaatct gacgatctgg ccatggtcca tcggccgctt
     1321 cgacgccatc atgaaggcgc cagaactggc ggattacgag atagtgcgct gatgtccgaa
     1381 agcaagacga aaaaacgcgg gggcttttac gacgggttcc tcgcgccggg cattaccacc
     1441 ggatacagcc cttgaccttg tcttcctctt ttcccgactg cgcgacgagc gagtcgacaa
     1501 tacagcggtt ggtgtaattc tgtttcatgt tgtcaggcat gttggcgaac atggagttgt
     1561 ctgccatttt cgcgttgcat tccacattgg ccttgtcaaa catctggtcg cactgctcgg
     1621 gcttggcacc caggcagtac agaaaatccg gccggccaca ctgctcatgg cgggcatcat
     1681 tgagcgcgca accagacagc aacagcgcac ccggcaggca gagagaccac aacagtttgt
     1741 tcatttgaat cagatgactc cattgtcctg ttacagccgc tccagccgga tcggcagatc
     1801 atctttcggc ttggcaatcg gcaccagttg atacggaatt tcatagtcac gtggcacgct
     1861 ccaacggaag cgcagcacca gctggtgcat gatcgccttc acctgcagat cggcaaaatg
     1921 ctggccgatg cacatgtggg caccaccccc aaacggtacc cacgcgaacg ggtgctgctt
     1981 gtgttcctcc cgcggcggcg caaagcgcag cggatcgaac gtatccggat cagtccagat
     2041 ttcttccata tagtgcgtgt gcaccggtga caccgacacc agcgtgttgg ctggaatcag
     2101 gtagccactg aactccaccg cgcgggtggt catgcgcggc atgctcggca atggcggata
     2161 catccgcaac gcttccttca tgcacagctc ggtcatggtc gcttccgcca gatcatcgaa
     2221 cgacagcgcg ggcttgttga agatgcgcgc ctccgcccgc actttttcct gccactccgg
//
LOCUS       DASUEG010000366         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_801328, whole genome shotgun sequence.
ACCESSION   DASUEG010000366 DASUEG010000000
VERSION     DASUEG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801328"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFX11_06825"
     CDS             complement(<1..559)
                     /locus_tag="VFX11_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX4938370.1"
                     /translation="MLQLRGAPALSAFRRTRLLNDLQSVVPAVTGLYAEFMHFADLGD
                     ALTAGEQQVLESILRYGPTEAVEKPVGQLLLVVPRPGTISPWSSKATDIAHNCGLAKV
                     KRLERGIAYHVAASAPLNTAQLQQLAGRLHDRMVEAVFTEMEEAARLFRVEDPRPLTT
                     VDVLGGGRAALATANKTLGLALAEDE"
     gene            complement(694..1833)
                     /locus_tag="VFX11_06830"
     CDS             complement(694..1833)
                     /locus_tag="VFX11_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938371.1"
                     /translation="MSAKGKSIAALAVPVVVQSGFLIWALSGTEGSGGMIAAGLVLGL
                     VAAGVGIMLLGQGNEPAEDLRFVEALLRGERRLSDRLPELDSRPLNRALNRWLENLQK
                     VFEGISGNLDRIKVMSRELSKVSGSADTGLEEDTRHVVDAVSSMTAAVHDASQNAATA
                     AQAAKDASQEAESGQKVVNSVTSSIHSLAGEVERAATAIQKLESDTEAIGAILEVIRG
                     IADQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLAQRTQEATKEINNMIARLQEGS
                     KNAVSVMAQGRRQAELSVEQASKAGESLQVITGAIGSISQMNEQIAAAVEQQTAAAEA
                     INGSLRRMGQSSGQGSRRSHELGRLKQDIENTALELEQIINRYVS"
     gene            2333..>3522
                     /gene="mltF"
                     /locus_tag="VFX11_06835"
     CDS             2333..>3522
                     /gene="mltF"
                     /locus_tag="VFX11_06835"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEX4938372.1"
                     /translation="MLAGFILTPACQPALPLLEQVKQQGELVVATRTGPTTYYREMDR
                     ETGFEFELAQAFANYLGVKMRMVAYDDLGELLTAVRTGKVHLAAAGLSVTPERSHQFR
                     FTSPYEQVTQDVIFRAGTPKPVSIEDLYQRKLVVLAQSSHSEYLQQLQQSHPELRWEE
                     KANATALTLLNMVNESQADVAVVDSAAFQTYRPLFPELQAAFPIKQAEPVSWAFQITA
                     DDSLFNMAELFFNQINASGDLEELRERFFGHREFDYVGARTFLTHLDTRLVEYEPDFK
                     AAADEVGMDWRLLAAVGYQESLWNPNAISPTGVRGLMMLTQQTATEMGVTDRRDAEQS
                     IRAGARYLKKLYDKLPQDLQEPHRTWFALAAYNVGYGHLMDARRLARHEGLDPNDWFV
                     VREKL"
BASE COUNT          707 a         1158 c          986 g          671 t
ORIGIN      
        1 tctcgtcttc cgccagtgcc aggcccagcg ttttgttcgc tgtcgccagt gcggcacggc
       61 ctccgcccag cacatccacc gttgtcagcg gacgcggatc ttccacccgg aacaggcgcg
      121 ccgcctcttc catttccgtg aacacggctt ccaccatgcg atcgtgcaga cggccagcca
      181 gctgctgcaa ctgggcagtg ttcagtggtg cactggcggc aacatggtag gcaatccccc
      241 gctccagccg ctttaccttg gccaggccgc aattgtgggc aatgtcagtg gccttggacg
      301 accaggggga aatcgtaccc gggcgtggca ccaccagcag caactggccc acgggttttt
      361 ccactgcttc ggtggggcca tagcgcagga tcgattccag cacctgttgt tccccggcgg
      421 tcaatgcatc ccccaggtcg gcaaaatgca tgaactcggc gtacaggccg gtgaccgcag
      481 gcacaacgga ttgcaggtca ttcagcaggc gggtacggcg gaaagcggaa agagcggggg
      541 caccacggag ttgcagcatg ggggtctcgg ccatcggggg attgaagggc gcacattata
      601 aaggatcgtc cgccggaagg gatcaagggc ggacaaaaga acaacgggcc tgcccgtcac
      661 ccgggaaggc ccgttatcaa acgcggcatc cgcttaactg acgtaacggt tgatgatctg
      721 ctccagttcc agcgcggtgt tttcgatgtc ctgcttcagg cggcccagct catggctgcg
      781 acggctgccc tgaccggagg attgccccat gcggcgcaga ctgccgttga tggcctctgc
      841 ggccgcggtc tgctgctcaa ccgcagcggc aatctgctca ttcatctgac tgatggaacc
      901 aatggcacct gtaatcacct gcagggattc acccgccttg ctggcctgct ccacactcag
      961 ttccgcctgc cggcggccct gggccatcac gctcactgcg tttttggagc cttcctgcag
     1021 ccgggcaatc atgttgttga tttccttggt ggcttcctgg gtgcgctgcg ccagagtacg
     1081 cacttcgtcg gctaccaccg cgaaaccgcg cccctgctcg cccgcccgtg ccgcctcaat
     1141 ggccgcgttc agtgccagca ggttggtctg gtcggcaata ccccgaatca cttccagaat
     1201 ggcaccgatc gcttcagtgt ccgattccag tttctggatg gcagtcgccg cccgctccac
     1261 ttcacccgcc agggaatgaa tcgagctggt cacgctgttc accactttct ggccgctttc
     1321 cgcttcctgg ctggcatctt ttgccgcctg ggccgcggtg gccgcgttct gggatgcgtc
     1381 gtgcacggcc gcggtcatgg acgacaccgc atccaccaca tgacgggtgt cttcctccag
     1441 gccagtgtcg gcactgcccg acaccttgct gagttcccgg ctcatcacct tgatccggtc
     1501 cagattgccc gagatgcctt caaatacctt ttgcagattc tccagccagc ggttcaacgc
     1561 ccggttcagc gggcgggaat ccagttcagg caagcggtcc gacagacgcc gctccccgcg
     1621 cagcagagcc tccacaaaac gcagatcctc cgccggctcg ttaccctgac ccagcagcat
     1681 gatcccgacg ccagccgcaa ccaggcccaa caccaggccg gcggcgatca tgccgccact
     1741 cccttcggtt ccggacagcg cccagatcag aaaccccgac tgaaccacca ccggcacggc
     1801 cagtgcggcg atggattttc ccttggcaga catagaatta gcctctctga tgtttcacga
     1861 caacagcccc gaccgacacg gcagccagtc tactgcggct tgtgcgccac ccgtcagtta
     1921 agcgtaggca aggccccgac aagctgcaaa gattcagcaa gtgtgacaaa aagcacagct
     1981 aaataactga ttagtatata taaagcctta gttatgacgg ttaaccggat tttttggtcg
     2041 acaagcctag aaggctgccc atataatccg gtcaaatatt aatcagcttc acgcagccac
     2101 tcccacagcc gtgcgcagcg tggaacttcg agtacacccg acaccattca aataggatga
     2161 aatccgcgtc tgtttccgtg gatggtgtct gacagggaag ataccgtcgg gccagcaatt
     2221 cctgcctcca ggcaagaaag cgctgaccgg acggattgac aggaaatcac cagacgcctg
     2281 cttgcttatg tctattattc gatggctgac tcgaaccacc ctgcggggtt tcatgctcgc
     2341 cggtttcatt ctgacaccgg cctgccaacc cgccctgccc ttgctggaac aggttaaaca
     2401 gcagggggaa ctggtcgtgg ctacccgcac tggccccacc acctactacc gcgaaatgga
     2461 ccgggagacc ggttttgagt tcgaactggc ccaggccttt gccaactatc tgggcgtgaa
     2521 gatgcgcatg gtggcttacg acgacctggg cgaactgctg acggcggtgc gaaccgggaa
     2581 agtccacctg gctgccgccg gcctcagtgt cacccccgag cgcagccacc agttccgctt
     2641 cacatcgccc tacgagcaag tgacacagga tgtgatcttc cgcgccggca cccccaaacc
     2701 cgtctccatc gaagacctct atcagcgcaa gctggtagta ctcgcccaga gcagccattc
     2761 cgaatacctg cagcaactgc agcagagcca tccggaactg cgctgggaag aaaaggccaa
     2821 tgccacagcg ctgaccctgc tgaacatggt gaatgaaagc caggccgacg tggccgtggt
     2881 ggactccgcc gccttccaga cctaccgccc cctgtttccg gagctgcagg ccgcattccc
     2941 catcaagcag gccgagcccg tatcctgggc gttccagatc accgccgatg actccctctt
     3001 caacatggcc gaactgttct tcaaccagat caatgcatcg ggcgatctgg aagaactgcg
     3061 ggaacgcttc ttcggtcacc gcgagtttga ttatgtcggc gcccgcacct tcctgaccca
     3121 cctggacaca cgactggtgg agtacgaacc ggacttcaag gctgccgccg atgaagtggg
     3181 catggactgg cgcctgctgg ccgccgtcgg ctaccaggaa tccctctgga atcccaacgc
     3241 catctcgccc accggtgtac gggggctgat gatgctgaca cagcagaccg caacggaaat
     3301 gggcgttact gatcgccgcg atgccgaaca gagcatccgc gccggtgccc gctacctgaa
     3361 aaaactctat gacaagctgc cgcaggatct gcaggaaccc caccgcacct ggttcgcgct
     3421 ggcggcctac aacgtgggct atggtcatct gatggatgcg cgccggctgg cacggcatga
     3481 aggactcgat cccaacgact ggttcgtggt gcgggaaaaa ct
//
LOCUS       DASUEG010000367         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_185392, whole genome shotgun sequence.
ACCESSION   DASUEG010000367 DASUEG010000000
VERSION     DASUEG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185392"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFX11_06840"
     CDS             complement(<1..946)
                     /locus_tag="VFX11_06840"
                     /EC_number="2.7.7.1"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003786.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HEX4938373.1"
                     /translation="MSHTFDFLVCIGRFQPFHNGHLAVVEAGLRQARQLILLCGSAHQ
                     PRSVRNPFSWQEREAMMRSAIAPGDQARLLVAPLADSPYSEDAWVRDVQRAVQDLVTA
                     HHQDRQRAPRIGLIGHCRAQSAYYPNRFPQWDAVAVEDVAGVNATAIRQSLLGPDFSP
                     GAVNAWLPPGVQQWLATFAAGAVCRDLREEQAFITAYRQGWASAPYEPNFVTVDAAVV
                     QSGHILLIERDRRPGRGLLALPGGFVDPGERILDACLRELVEETCIGVPLPVLRGCIK
                     QSAVFDAPHRSARCRTITHAFHIELAPGRNLPSVSGSDD"
     gene            complement(1052..2209)
                     /locus_tag="VFX11_06845"
     CDS             complement(1052..2209)
                     /locus_tag="VFX11_06845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4938374.1"
                     /translation="MDFFAWINRRFGNPLEWSSAAKATMLMAATLAMHGQYTLWAHYC
                     LAHPEFVGEAIQAEFLQGELPVFHGLLLTTFLLCLVLPLLWRLFGDSTLYEYLAANYY
                     GLSHCYFAWLIGTLSLPTGIVMAGAPVLGFIFFNRSAVVIAFINSALALLTISLLSAF
                     GSLPYAPVIRQFTSPDGQVLVFWAGNFIFYSVPHLLFLFGLAYLVLRRWREREDQAHT
                     MSRIDPLTGLLNRRSILIQLGREQERSVQQDSTMAVVMVDLDHFKQINDTWGHEAGDY
                     VLIAAAEVLQKNVRQNDRVGRYGGEEFLLVLPGADLEGARLLAERCRQQLETLEVMLF
                     DGRRLRVSASMGLCCNQRERTLTPEMMLRRADKALYVAKETGRNRVVAVDD"
BASE COUNT          541 a          781 c          643 g          356 t
ORIGIN      
        1 cgtcatcact gccggacact gacggcaaat tccggcccgg cgccagctcg atatgaaagg
       61 catgggtaat cgtgcgacag cgggccgagc gatggggcgc gtcaaacacg gcagattgtt
      121 tgatacagcc gcgcaacacc ggtaacggca cgccgatgca ggtttcctcc accagttccc
      181 gcaggcaggc atccagaata cgttctcccg gatcgacaaa gccgcctggc aatgccagca
      241 ggcccctgcc cggtcgccgg tcgcgctcga tcagcaggat atgccccgac tgcaccaccg
      301 ccgcgtccac ggtgacgaaa ttcggctcat aaggcgcgct cgcccagccc tggcgataag
      361 cggtgatgaa tgcctgctcc tcgcgcagat cacgacagac agcgccggca gcaaaggtcg
      421 ccagccactg ctgcacaccg ggcggcagcc acgcattcac cgcaccgggc gaaaaatccg
      481 gacctagcag tgactggcga atggccgtgg cattcacgcc ggccacatcc tcaaccgcca
      541 ccgcgtccca ctgcggaaac cggttcgggt aatacgccga ctgcgccctg caatggccga
      601 tcaaaccaat gcgcggcgcg cgctgtcgat cctgatgatg ggcagtaacc agatcctgca
      661 ccgcgcgctg cacatcacgc acccaggcat cctcgctgta aggcgaatcc gccagcggcg
      721 cgaccagcag gcgggcctga tcccccggcg caatggcact gcgcatcatc gcctcacgtt
      781 cctgccagct gaacggattg cggacggaac gcggctggtg ggcggaaccg cacagcagaa
      841 tcagctgccg tgcctgccgc aacccggctt cgaccacagc gagatggccg ttgtgaaacg
      901 gctggaaccg accaatgcaa accagaaaat caaatgtgtg cgacatgcag gcgccttgct
      961 gtttgaatgc gggaagcggg ctgaaccgcg ccgccaccat agccaatccg cacttgcgcg
     1021 ggcaacgaat attttcggga aaatgtcgcg ctcagtcgtc cacggccacc acgcgattgc
     1081 ggcccgtttc cttcgccaca tagagcgcct tgtcggcacg gcgtaacatc atttccggcg
     1141 tcagcgtccg ttcccgctga ttgcagcaca gccccatgct ggcggatacc cgcaggcgcc
     1201 ggccatcaaa cagcatcact tccagcgtct ccagctgctg ccggcaacgt tccgccagca
     1261 gacgcgcgcc ttccagatcc gcgcccggca gaaccagcag aaattcctcg ccgccatagc
     1321 ggcccacgcg atcattctgg cgcacgtttt tctgcagcac ttccgccgcc gcgatcagca
     1381 cataatcgcc cgcctcgtgg ccccaggtat cattgatctg cttgaaatga tccagatcca
     1441 ccatcaccac cgccatcgtc gaatcctgct gcacactgcg ctcctgctcg cggcccagct
     1501 ggatcaggat actgcgacgg ttgagcaggc cggtaagcgg atcgatgcgg ctcatggtgt
     1561 gcgcctgatc ctcgcgctcg cgccagcgcc gcagcaccag atacgccagc ccgaacagaa
     1621 acagcagatg cggcaccgaa tagaaaatga aattgccggc ccagaacacc agcacctgac
     1681 catcgggact ggtgaactgg cggatgacag gcgcgtacgg caggctgcca aacgccgaca
     1741 gcagcgaaat cgtcagcagc gccagcgcgg aattgataaa cgcgatcacc acggcggagc
     1801 gattgaaaaa aatgaaaccc aataccggtg caccggccat cacaatgccg gtaggcaacg
     1861 acagggtgcc gatcagccac gcgaaataac aatgggacaa accgtaataa ttggccgcca
     1921 gatattcata cagcgtgctg tcgccaaaca ggcgccacaa cagcggcagc accagacaca
     1981 acaggaaggt cgtcagcagc aaaccatgaa atactggcag ttcgccctgc agaaattcgg
     2041 cctggatggc ctcacccaca aattccggat gggccaggca ataatgcgcc cacagggtat
     2101 actggccgtg catcgccagc gttgccgcca tcagcatggt cgccttggcg gctgaactcc
     2161 attccagcgg attgccaaac cgccggttta tccaagcaaa gaaatccatc ccaacgccaa
     2221 ctcgcaagac cacagccaat caagacaatt gaccgattat agcggaagtg cgtgttaatt
     2281 aggcagcggg gcagcgccat acgccgggat catccgacga a
//
LOCUS       DASUEG010000368         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_841384, whole genome shotgun sequence.
ACCESSION   DASUEG010000368 DASUEG010000000
VERSION     DASUEG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841384"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..98
                     /locus_tag="VFX11_06850"
     CDS             3..98
                     /locus_tag="VFX11_06850"
                     /inference="COORDINATES: protein motif:HMM:NF023573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan-binding protein"
                     /protein_id="HEX4938375.1"
                     /translation="MCPNAANNAKWNGQWTNINASAGSVCGCIAQ"
     gene            complement(252..2486)
                     /locus_tag="VFX11_06855"
     CDS             complement(252..2486)
                     /locus_tag="VFX11_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938376.1"
                     /translation="MWNPRLLALLVPLLVLSGCKSARETEPAYTLAQQCVAIQNPASG
                     AYLAAASDKAYRFSAGGEEQAEKFFLKPSALGTFLLYDRDGRFLSTQLIHVTRDATAS
                     RRAEWRINKLALYRGHRQVDEKFTLVSIIDNLRLQQGKSGPIIRLAGEIVPEEAVLDL
                     VPQPADACREFPEAPLNAEVADDFYQPKDPAAPVTGYADLHTHLGFPQAMAGLAMAGD
                     LFHPWGIEHALKDCAHLHGANGMLDLLEGQRASGGAGGHATAGYPDLTYWPRRDTSTH
                     VQAYYRWLQRAYLGGLRLMVSHVTGNPTFCQLLSLMKPGRAEGDCSSRSEVENQSLYL
                     YALQDYIDAQEGGPGKGWFRIATSAREARAIINQNKLAVVLGAEYGTLFDCNESATFC
                     SPQYIDQQLERIHQLGVRVVFPIHRFDNAFGGTRPAGDVAGAWMNLASKLNTSRAPKV
                     TQMLDPFGYLFKPIGGHFWELQTCPDGIEGTSGLLSMEQFINQDFRALTNGLADIPTI
                     GPTLNSLLELGFFSKLNPLPDYAGFDQPDSHACNLTPLQPAGHYLVNRLIDKGMIIDI
                     DHMGYGTQMDTLDILEQRQYAGVVSSHSWMESNAQIRARMFRLGGQISPFNHRPSDNA
                     AVMKEYRAEMAAFDFPIGVGMGSDIQGVTAQTEGDPEIAITYPFQSVDGKVSFTQPTT
                     GNRVFDYATEGVANYGMMAEWVENLRQLDANDPDDLMGIFMNSAESFLQMWERAEAAK
                     MDLD"
BASE COUNT          457 a          761 c          891 g          552 t
ORIGIN      
        1 gggtgtgtcc gaatgcagca aacaatgcca aatggaacgg gcagtggacc aatatcaatg
       61 ccagcgccgg ttcggtttgc ggatgtattg cccaatagcg acgcggagtt cggctcaaca
      121 gcttcgttga gccgaactta tctgagaaat gcacgtgttg aattgcatgt ccaattgacc
      181 cactgatatc gggtttacga gctgaagcgt caagcaattg gtccgatgag tacgcgtcaa
      241 cagcaggtgc gttaatcgag gtccatcttc gccgcttccg cccgttccca catctgcaag
      301 aacgattcgg cggaattcat gaaaatcccc atcagatcgt ccggatcgtt cgcgtccagc
      361 tggcgcaggt tttccaccca ttccgccatc atgccgtagt tggcaacgcc ctcggtcgcg
      421 taatcaaaca cgcggttgcc ggtggtcggt tgcgtgaagc tgaccttgcc atccacggat
      481 tgaaacggat aggtgatcgc tatttcggga tcgccttcgg tctgcgcggt gacaccctgg
      541 atgtcgctgc ccatgccgac gccgatggga aaatcaaacg cggccatttc cgcgcggtat
      601 tccttcatca cggcggcgtt gtcgctgggg cggtggttga aggggctgat ctgtccgccc
      661 aggcggaaca tgcgcgcgcg gatctgtgcg ttgctttcca tccagctgtg ggatgacacc
      721 acgccggcat attgccgctg ctccagaata tccagcgtgt ccatctgggt gccgtatccc
      781 atgtggtcga tgtcgatgat catgcccttg tcgatcagcc ggttcaccag gtagtgcccg
      841 gccggttgca gcggcgtcag gttgcaggcg tggctgtcgg gctgatcgaa tccggcgtag
      901 tccggcagcg ggttgagttt gctgaagaag cccagctcca gcagcgaatt cagggtgggg
      961 ccgatggtgg gaatgtcggc caggccattg gtgagggcgc ggaaatcctg gttgatgaac
     1021 tgttccatgc tgagcaggcc ggaggtgcct tcaattccgt caggacaggt ttgcagttcc
     1081 cagaaatggc cgccgatggg tttgaacagg tagccgaacg ggtccagcat ctgggtgact
     1141 ttcggcgcgc ggctggtgtt gagtttgctg gccaggttca tccaggcgcc ggccacatcg
     1201 ccggccgggc gggtgccgcc aaaagcgttg tcgaagcggt ggatggggaa caccacgcgt
     1261 acgcccagct ggtggatgcg ctccagttgc tggtcgatgt actgcgggct gcagaacgtc
     1321 gcgctttcgt tgcagtcgaa cagggtgccg tattcagcgc ccagcaccac cgccagtttg
     1381 ttctggttga tgatcgcgcg ggcctcgcgg gccgaagtgg cgatgcggaa ccagcccttg
     1441 ccggggccgc cttcctgggc gtcgatgtag tcctgcaggg cgtacagata gagcgactgg
     1501 ttttccactt ccgagcggct actgcagtcg ccctcggcgc ggcccggttt catcagcgac
     1561 agcagctggc agaaggtggg attgcccgtg acgtgggaca ccatcagccg cagcccgccc
     1621 agataggccc gctgcagcca gcggtaatag gcctgcacgt gggtgctggt gtcgcgccgg
     1681 ggccagtagg tgaggtcggg gtagcccgcc gtggcatggc cgccggcacc gccgctggcg
     1741 cgctggcctt ccagcaggtc gagcatgccg ttggcaccgt gcaggtgggc gcagtctttc
     1801 agggcgtgtt cgatgcccca gggatggaac agatcgcccg ccatcgccag cccggccatg
     1861 gcctggggaa agcccagatg ggtgtgcagg tcggcgtaac cggtcaccgg tgcggccggg
     1921 tctttgggtt ggtaaaaatc gtcggccact tctgcgttca gcggcgcttc gggaaattcg
     1981 cggcaggcgt ccgccggttg cggcaccaga tccagcacgg cttcctccgg cactatttcg
     2041 ccggcaaggc ggatgatcgg cccgctcttg ccctgctgca ggcgcaggtt gtcgatgatg
     2101 gacaccaggg tgaatttctc gtccacctgc cggtggcccc ggtacagcgc cagtttgttg
     2161 atgcgccatt ccgcccgccg cgacgcggtg gcatcgcggg tgacgtggat cagctgggtg
     2221 ctgaggaagc ggccgtcgcg gtcatacagc aggaaggtgc ccagggcgct gggtttgagg
     2281 aagaattttt ccgcctgctc ctcgccgccg gcgctgaaac ggtaggcctt gtcactggcg
     2341 gcagcaaggt aggcgcccga ggccggattc tggatggcca cacactgctg ggccagggta
     2401 taggccggtt cagtttcacg ggcggatttg cagccggaca acactaacag cggcacgagc
     2461 agggccagca gacggggatt ccacatggac ggtaccttcc tggtttgttg ttattggtgg
     2521 aagtggcgga tcatcacagt tgctgtacga tggtgccatt gtgtttttag gggatttacc
     2581 ggttcccgtc aaatcctgct gcggccgggt cgctgacggc aaaatgaccg tggtaccatc
     2641 gcccccgttc atgcggggca t
//
LOCUS       DASUEG010000369         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_70612, whole genome shotgun sequence.
ACCESSION   DASUEG010000369 DASUEG010000000
VERSION     DASUEG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70612"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VFX11_06860"
     CDS             <1..1604
                     /locus_tag="VFX11_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX4938377.1"
                     /translation="ARLIDDLATDQADMIAKAKAWCKANPNAVQPWDDKSYKVPGGTP
                     SSPGLAMKLPISPAMLKSKTKGCFPAPEAILSAAVESLQVDFDTAMKVEARYFTSLAV
                     GPVAKNMIKAFFFQLQEIGKGASRPKGIEHHTTKKVGILGAGMMGAGIAYSSAMSGIE
                     VVLKDISLEAAQKGKAYSENLLKKRISKGAMTQEKADKVLSLIKPTANAEDLAGCDLV
                     IEAVFEKRELKAQVTKEAEAQMLATGVMASNTSSLPITGLAEASVRPANFIGLHFFSP
                     VDKMPLVEIICGKQTSPETLAKSIDYVLQIKKTPIVVNDARGFFTTRVIGTYVNEGLG
                     MLKEGLAAASIEMAAAKAGFPVGTLAISDELNLQTMRNISLAARADLEAQGQTLPVTP
                     SEEVIVKMLDLKRAGKAAGAGFYDYPQGGKKTLWPGLKDVFAAGSKQIPFEDMQERFL
                     FVQALETVRCMQEGVIEKVADANIGSIMGIGFPAWTGGVVQYINQYGLRKFVARAEEL
                     AKRYGERFNPPALLVEKAEKDEIFE"
     gene            1717..3138
                     /locus_tag="VFX11_06865"
     CDS             1717..3138
                     /locus_tag="VFX11_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938378.1"
                     /translation="MRQYCKFHPLEPALWYNPRAHMAYCERCVDSGETRGGLGQARCY
                     LTGDELQYLGSANTAKPFWEVLGQFFRYPLKPDSLMMIGIFVVASWAVLSLMTSTGII
                     LMLVGALLILACITRYGFLILEYTAEGRAEPPTLSETFASAGFEVLFQQVAVQIVFVA
                     FLVLVGRLGSAFFDVIALALVVFVTPASLMILATEKSITLAINPQSISHLIRSIGWSY
                     LLLYAFLFLLWGAEAAVFEVFADEISAQFFLPVFVGVTLYFMMVAYNLMGYVIFQYQS
                     AIGFVAEDAVAREKRRSTIDPLDAKVEVLVKEGRYDKAVETLSKHLRTQPGSVRHHEK
                     LARLLIAMNDREQALAHAQPYMEAVHKLGDDSRLYFLYNDYEKLDPKFLPEAPAICVS
                     LAHQLYRRGKFNQTCHLIANLHKRAPNFADIPDAYLLMARALFDGLKDSYKAAQYLKF
                     IKAKYPDFKEMSQVDKLLAECGA"
     gene            3197..>3599
                     /gene="amrS"
                     /locus_tag="VFX11_06870"
     CDS             3197..>3599
                     /gene="amrS"
                     /locus_tag="VFX11_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX4938379.1"
                     /translation="MTETKDPTLTRDSPFLHATRFWHRLDDGRVQCDVCPRECKLHEG
                     QRGLCFVRARLDDAIVLTTYGRSSGFCVDPIEKKPLNHFYPGTSVLSFGTAGCNLACK
                     FCQNWDISKSRDMDRLMDAAAPDEIARTASHW"
BASE COUNT          743 a         1009 c         1115 g          732 t
ORIGIN      
        1 ccgcgcgcct gatcgacgat ctggccactg accaggctga catgattgcc aaggcgaaag
       61 cctggtgcaa agccaatccc aatgccgttc agccctggga tgacaagagc tacaaggttc
      121 ccggcggtac tccgtccagc cctggcctgg ccatgaagct gccgatcagt ccggccatgc
      181 tgaagagcaa gaccaagggt tgtttcccgg cgccggaagc gattctgagc gcagccgtgg
      241 aatccctgca ggtggatttc gatactgcga tgaaagtgga agcccgttac ttcaccagtc
      301 tggcggttgg cccggtggcg aagaacatga tcaaggcatt cttcttccag ctgcaggaaa
      361 tcggcaaggg cgccagccgt ccgaaaggca tcgagcacca taccaccaag aaagtgggca
      421 ttctgggtgc gggcatgatg ggcgcgggca ttgcgtactc cagcgccatg tcgggcattg
      481 aagttgtgct gaaggacatt tcgctggaag cggcgcagaa aggcaaggcg tacagcgaaa
      541 acctgctgaa gaagcgcatt tccaagggcg ccatgactca ggaaaaggcc gacaaggtgc
      601 tgagcctgat caagccgact gccaatgctg aagatctggc gggttgcgat ctggtgatcg
      661 aagccgtgtt cgagaagcgt gaactgaaag cgcaggtgac caaggaagca gaagcgcaga
      721 tgctggccac tggcgtgatg gcgtccaaca cttccagcct gccgattacc ggtctggcgg
      781 aagcgtccgt gcgtcctgcc aacttcatcg gtctgcactt cttctcgccg gtcgacaaga
      841 tgccgctggt tgagatcatc tgtggcaagc agacctcgcc ggaaaccctg gccaagtcca
      901 tcgactacgt tctgcagatc aagaagaccc cgattgtggt caacgacgcg cgcggcttct
      961 tcacgacccg cgtgatcggc acctacgtga acgaaggtct gggcatgctg aaggaaggcc
     1021 tggcggcagc ttccatcgaa atggcggcgg caaaagccgg cttcccggtg ggcaccctgg
     1081 cgatcagcga cgaactgaac ctgcagacca tgcgtaacat cagcctggca gccagagccg
     1141 atctggaagc acagggccag acattgccgg tcaccccttc cgaggaagtg atcgtgaaaa
     1201 tgctggacct gaagcgtgcc ggcaaagcgg caggcgcggg cttctacgac tacccacaag
     1261 gtggcaagaa gacgctgtgg ccgggcctga aggacgtgtt cgctgcgggt tccaagcaga
     1321 ttccgttcga agacatgcag gagcgtttcc tgtttgtgca ggcgctggaa accgtgcgct
     1381 gcatgcagga aggcgttatc gagaaagtgg cggacgccaa catcggttcc atcatgggga
     1441 tcggcttccc ggcctggacc ggcggcgtgg tgcagtacat caaccagtac ggcctgcgca
     1501 agtttgtggc ccgtgcggaa gagctggcca agcgctatgg cgagcgtttc aatccgcccg
     1561 cgctgctggt ggaaaaagct gagaaagacg agatcttcga gtaatccgcg aagctcggcc
     1621 cgctctggcg ggaacggaac ggcccgccaa aagcgggccg tttttgtttg tgcgcggcta
     1681 taatgtctga cagatcagtc atctgccgga accttcatgc gccaatactg caaatttcac
     1741 ccgctggaac ctgctctctg gtacaacccc cgagcccaca tggcctattg cgaacgctgc
     1801 gtcgacagcg gcgaaacccg gggcgggctg gggcaggcgc gctgctatct gaccggcgat
     1861 gagctgcagt acctgggcag cgccaacacc gccaagccat tctgggaagt gctcggccaa
     1921 ttctttcgtt acccgttgaa gccggatagc ctgatgatga ttggcatctt cgtggtggct
     1981 tcgtgggcgg tgctgagcct gatgacgtcc accggcatta tcttgatgct ggtgggggcg
     2041 ctgctgatcc tggcctgtat cacgcgctac ggttttctga tcctggagta cacggcagag
     2101 ggccgcgccg agccacccac gttgagtgaa acgttcgcca gcgccggttt cgaagtgctg
     2161 ttccagcagg tggcggtgca gatcgtgttc gtggctttcc tggtgctggt gggccggctg
     2221 ggcagtgcct ttttcgatgt catagccctg gccctggtgg tatttgtcac gcccgccagc
     2281 ctgatgatcc tggccacgga aaaatcgata acgctggcca tcaatcccca gtctatttcc
     2341 catctgattc gcagtattgg ctggtcgtat ctgttgctgt atgcctttct gtttttgttg
     2401 tggggagcgg aggcggcagt gttcgaagtg ttcgccgacg aaatctcggc gcagtttttc
     2461 ctgccggtat tcgtcggagt aaccctgtat ttcatgatgg tggcgtacaa cctgatgggg
     2521 tacgtgattt tccagtatca gtctgccatc ggttttgtgg cggaggatgc ggtggcgcga
     2581 gagaaacgcc gcagcactat cgatccgctg gatgccaaag tggaagtgct ggtgaaggag
     2641 ggtcgctatg acaaggcggt ggaaacgcta tcgaagcatc tgcggaccca gccgggcagt
     2701 gtgcgacacc atgaaaaact ggcgcggctg ctgattgcaa tgaatgaccg cgagcaggca
     2761 ttggcccatg cgcagcctta catggaagca gtgcacaagc tgggagacga ttcccgtctg
     2821 tactttcttt acaacgatta cgagaagctg gaccccaaat ttctgccgga agcgcccgcc
     2881 atttgcgtat cgctggcgca ccagctttac cggcggggaa aattcaacca gacctgtcac
     2941 ctgatcgcga atctgcacaa gcgggcgccc aactttgctg atattccgga tgcctacctg
     3001 ctgatggcgc gggcgctgtt cgacggtttg aaggattcgt acaaggccgc gcagtatctg
     3061 aaattcatca aggccaagta cccggatttc aaggaaatgt cccaggtgga caagctgctg
     3121 gcggagtgcg gagcttgaaa atcgccgtcg ccgggcgcat ataggggaaa gatccattcc
     3181 cgtccggtga gcgccagtga ctgaaaccaa agatcccacc ctgacacgtg acagcccgtt
     3241 tctgcacgca acccgcttct ggcatcggct cgatgatggc cgtgtgcagt gcgatgtctg
     3301 cccgcgtgaa tgcaagttgc acgagggcca gcgtggcctg tgtttcgtgc gcgcgcggct
     3361 ggacgatgca attgttctga ccacctacgg ccgctccagc ggtttctgtg tcgatcccat
     3421 cgagaaaaaa ccgctcaatc atttttatcc cggcacgtcg gtgttgtcgt ttggtacggc
     3481 cggctgcaat ctggcgtgca agttctgcca gaactgggac atttccaagt cgcgcgacat
     3541 ggaccggctc atggacgccg ccgcgccgga cgaaattgcc cgcaccgcct cccactggg
//
LOCUS       DASUEG010000370         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_598351, whole genome shotgun sequence.
ACCESSION   DASUEG010000370 DASUEG010000000
VERSION     DASUEG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598351"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFX11_06875"
     CDS             complement(<1..901)
                     /locus_tag="VFX11_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938380.1"
                     /translation="MTAPECPIWVKEEAFIQHILKLLLRKAETGHKPRVRISEKIAWC
                     APLFDFNHNQGPRLFGLLQLLAERDFDLLQIRLSSKRKKQFETEYEGAHVELKPLSIP
                     VLRHWLELPAYDEFPLSMQDAVYRQRTAFADQGASLLAAMHQLKVPGKSADDVIAAFT
                     RISGRAESCLSLRELSASCFWGDSKYLDGRQDLLIKLFPQVMQSLTRRARLLQVKLPT
                     EIEGVLIIENQDTFVRACLGQLPGADNLAIVYGAGFRATADSIRQRGSVMFACVETAD
                     FQSVAASAMRAQFESDWFHPEDRW"
     gene            complement(898..>2970)
                     /locus_tag="VFX11_06880"
     CDS             complement(898..>2970)
                     /locus_tag="VFX11_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX4938381.1"
                     /translation="SYPPGVEVALKAIRAQCPDADPRVLCDYIDVLDPQWQMAIEGYL
                     GGARFSIIVQPEYEAEAIRIVRNLPGQRNSARVIQGSKAQRDAERFQPAADSIIHILK
                     FQHKTAEYYLRASYGSVVRVPDAETLRSTARGITSNGLASGNYSMWRCDIPDAELVFG
                     QSARERALVAKQKELETLLAGMTQRQQQVEAAQQLFKAVSSVQGISFGRTLQEMLDIH
                     RQLKRSEQALAQLDTGDFERLEAELVQTRQRFAELEADLKQLENDKGRTQEQLNAATR
                     AAEALDKDKEKLQHDAERHEAAVRQIQHWQPQCDVDQLLLDADAQLTVDDHSFYRDEL
                     NNIDKILQQDANNLFNTIMQHNQRCNPFDAIVYDPDFSQLHEPAFFKRISDLVAEVDN
                     IRNRLQNNVLVEKHEHLTQLRDRFNETFVSHLCHSIYQAINEGERALKDLNRELEHHR
                     FGTDQERFYFAWDWVPEFKEYWRFFKEVSQLPNQGDGVSLFDMELPKAVQSVREKLVA
                     MLLDDDEQKAFRELQRIADYRHYRNYEIYKEPLGKAPIPLSQYGTGSGGQLETPAYII
                     RSAAITSAFRFNEGNTHLRMVLVDEAFSKMDETRSREVISYLTDTLGLQLVFIMPTSK
                     SGPFMDIISNQTVFSKCQSNRKIGELNTRVLVDRKECNQERIKALWQQQRTSIREQAT
                     LDFMEGIE"
BASE COUNT          573 a          872 c          856 g          669 t
ORIGIN      
        1 gccaacggtc ttcaggatgg aaccagtcac tttcaaactg cgcacgcata gccgatgcgg
       61 ctacggactg gaagtcagca gtttccacac aggcaaacat cacactgccc cgctgacgaa
      121 tgctgtcggc cgtcgcgcgg aaaccggcgc cgtacacgat tgccagattg tcagcgcccg
      181 gcaactgacc caggcaggca cgcacaaacg tgtcctggtt ttcaatgatc agcacgcctt
      241 cgatctctgt cggcaacttc acctgaagca aacgggcacg ccgtgtcagc gattgcatga
      301 cctgggggaa cagctttatc agcaagtcct gccggccgtc gagatatttg gaatcccccc
      361 agaagcaact ggcagacaac tcccgcaacg acagacagct ctctgcacgc ccggaaatcc
      421 gggtaaaagc cgcaatgaca tcgtcagcgg acttgccggg cactttcagc tggtgcatgg
      481 ccgccagtag cgaggccccc tggtcggcaa atgccgtgcg ttggcgatac accgcgtcct
      541 gcatgctgag gggaaattca tcgtaggcag gcaattccag ccaatggcgc aacaccggaa
      601 tcgacagggg tttcaattca acatgggcgc cttcgtactc ggtttcaaat tgtttcttgc
      661 gctttgatga caggcggatc tgcaacagat caaaatcgcg ctctgccagc aattgcaaca
      721 gtccgaacag gcgtggccct tgattgtgat tgaaatcgaa cagcggcgcg caccaggcga
      781 tcttctcgct gatgcgcacg cggggcttgt gtcccgtctc cgccttgcgc aacagcagct
      841 tcaggatgtg ctggatgaac gcctcttcct tcacccagat cgggcactcc ggcgccgtca
      901 ttcaatgcct tccatgaaat ccagcgtcgc ctgttcgcga atcgatgtgc gctgctgttg
      961 ccacaatgcc ttgatccgct cctggttgca ttccttgcgg tccaccagca cgcgggtgtt
     1021 gagttcgccg atcttgcgat tgctctggca tttggaaaac acggtctggt tggaaatgat
     1081 gtccatgaac ggaccggatt tgctggtggg catgatgaac accagttgca ggcccagcgt
     1141 gtcggtcaga tagctgatca cttcgcggga acgggtctcg tccattttgg aaaaggcttc
     1201 gtccaccagc accatgcgca ggtgcgtatt gccttcgttg aagcggaagg cggacgtaat
     1261 cgcagcagag cggatgatat acgccggcgt ttccagctga ccgcccgacc cggtgccata
     1321 ctgactgagc ggaatcggtg ccttgcccag tggttccttg tagatttcat aattccggta
     1381 atggcggtaa tcggcaatac gctgcagttc gcggaaggct ttctgctcgt cgtcatccag
     1441 cagcatggcc accagttttt cccgcacgga ttgcactgct ttcggcaact ccatatcgaa
     1501 caggctgacg ccatcgccct gattcggcag ctgcgacact tccttgaaga aacgccagta
     1561 ttccttgaat tcgggcaccc agtcccaggc gaaatagaag cgttcctgat cggtgccgaa
     1621 gcggtgatgc tccagctcgc ggttgagatc tttcagcgcc cgctcgcctt cattgatggc
     1681 ctgatagatg ctgtggcaca ggtggctgac gaaggtttcg ttgaaacggt cgcgcagttg
     1741 cgtcaggtgt tcgtgttttt ccaccagcac gttgttttgc agacggttgc ggatgttgtc
     1801 cacctccgcc accagatcgc tgatgcgttt gaagaacgcc ggttcgtgca gctgggaaaa
     1861 atccggatcg taaacaatgg catcgaacgg attgcagcgc tggttgtgct gcatgatggt
     1921 gttgaacagg ttgttggcat cctgctgcag gattttgtcg atgttgttca attcgtcgcg
     1981 gtagaagctg tggtcgtcca cggtgagctg cgcatcggca tccagtaaca gctgatcgac
     2041 atcgcactgg ggttgccagt gctggatctg gcgcaccgcc gcttcatgac gctcggcgtc
     2101 gtgctgcagt ttttccttgt ccttgtccag cgcctcggca gcgcgggtgg cggcattcag
     2161 ctgctcctgg gtgcggccct tgtcgttttc cagctgtttc agatcggctt ccagttcggc
     2221 gaaacgctga cgggtttgca ccagttccgc ttcgaggcgt tcgaaatcgc cggtgtccag
     2281 ttgtgccagc gcctgctccg agcgcttgag ctgacgatga atatccagca tttcctgcag
     2341 ggtgcggcca aaactgatgc cctgcacgct gctcacggcc ttgaacagtt gctgggcggc
     2401 ttccacctgc tgctggcgct gggtcatgcc ggccagcaga gtttccagtt ctttctgctt
     2461 ggccaccagt gcgcgctcgc gtgcactctg gccgaagacc agttctgcat cgggaatgtc
     2521 gcagcgccac atgctgtaat taccggacgc cagaccattg ctggtaattc cgcgcgcggt
     2581 ggagcgcaac gtttccgcat ccggcacccg caccacgctg ccgtaactgg cgcgcaggta
     2641 atattcagcg gttttgtgct ggaatttcag aatgtgaatg atggaatccg ccgccggctg
     2701 gaaccgctcg gcatcacgtt gcgccttgct gccctggatc acccgcgcac tgttgcgctg
     2761 gcccggcaga ttgcgcacga tgcggatggc ttcggcttcg tattccggct gcacaataat
     2821 ggaaaaccgc gcaccaccca gatagccttc gatagccatc tgccactgcg gatcgagcac
     2881 gtcgatgtaa tcgcacagca cccgcggatc ggcatcgggg cactgggcgc gaatggcttt
     2941 cagcgccact tccacaccgg gtggatagga
//
LOCUS       DASUEG010000371         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_294919, whole genome shotgun sequence.
ACCESSION   DASUEG010000371 DASUEG010000000
VERSION     DASUEG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294919"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..2881
                     /locus_tag="VFX11_06885"
     CDS             569..2881
                     /locus_tag="VFX11_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4938382.1"
                     /translation="MNLVDEATLGKHLMFLEDPQGVLLLDDILTDDYQSRFEPVFVDT
                     PYFGRTRSTYWFRLQVAPNKANNPRLIEIAYPHLKSITLYEMKDRRLLRTLQSGYENR
                     DMEPYNCLRFCFLIKGNHGIHTLYFRISTDSPMFVPMVMRAPEKQLLNERINMAVVAL
                     FYGAFVVMGLFNLFLFFSTQDRSYLFYVMYIVTLFLWTSSHDGLLREVLLYRNDMLSS
                     YRVHFLLTLVPILFGALFCQRFLRTAETMPLHHRVFSFLILLCLVDAALTLVSGRILL
                     PDSVNLLTVTFSIAGVSASVQGLRQGLRTARFFLTAWVAVILGSVLWVLTLSGVLPFN
                     SFTAFSVHIGGLLETILLSLALGDRINMLQAERIQLEQDAKRRLEDSNQKLEASNRFK
                     DEFLSTVSHELRTPMNGIIGASELLEHTDLNSEQYKYLSTINRSSHDMLGMIENILTY
                     TQFEAGTATAANRPMKIRQLLDEMTAHYRGRAAIKQLEFRHHIDPSIPSDLAGDYDKV
                     KLLLDQILDNAIKFTDSGHIRFDVTVDEERKHKDTLWLKFVIRDTGCGVPESLQGEIF
                     NSFKQGDGSMTRRKGGLGIGLALSKRIIDILDGRLTFKSSVLDGTTVILALPFRPCAA
                     QDEPAQADLAESINCASVEILVVEDNYVNKLVLEGFLKKLGFSVQAVDNGKEAVALLE
                     KREFDLVFMDCQMPVMDGFEATRQLRKLPNRNAGIPVVAVTANANPGDRERCLAAGMN
                     DYLKKPFSQQLLVSTLNRWLQRQRNDSVQA"
BASE COUNT          624 a          891 c          818 g          612 t
ORIGIN      
        1 gttcgcggtt ctggatcgaa tcgagcacag cctgcaactg ctggcggtag gtcagggtgc
       61 taccgggtat cctgccctgc cggatggtcg tgacaatggt gagttcattc actcccccca
      121 agtaacggga gtcctggatc agatgcatcg tggcacctcc ggtgctgggt tggccgtcaa
      181 tttgtgaatt cgttgggcaa ataaatgtga cgcaattcag atcacatcat aagaataaaa
      241 acaattccaa attatgcctt gtgaaaaatt cacactcatt gcggctgcgg attcgccccg
      301 aaattgaaca caaaacagcc agccgtgcac gatcgcgttc atttgattga tctatttaca
      361 aaaatacact tcaacgaatg accgctggtc aaattactga ctagactttg tttggattga
      421 tcgtgggttg tcggaaacaa tgctgtcatc gggaaacaac tggaatcgaa tctgctcacg
      481 ggggaaaaaa ctgtgggcgc tgctacctct gttgctgctg tttgcaacgg cattgacccg
      541 tgccagtacc agcctggaac ctctcgacat gaacctggtc gacgaggcca cgctgggcaa
      601 acacctgatg tttctggaag acccccaggg cgtgctgctg ctggatgaca tcctcacaga
      661 cgactaccag agccgctttg aaccggtctt cgtggacacc ccctatttcg gccgcacccg
      721 ttccacttac tggttccgtc tgcaggtggc gcccaacaag gcgaacaacc cgcgcctgat
      781 cgagatcgcc tatccccatc tcaagtccat caccctgtat gaaatgaaag accgccggct
      841 gctgcgcacc ctgcagtccg gctatgaaaa ccgcgacatg gagccctaca actgcctgcg
      901 gttctgtttc ctgatcaagg gcaaccacgg catccacacg ctgtacttcc gcatcagtac
      961 cgattccccc atgttcgtgc cgatggtgat gcgggcgccg gaaaagcagt tgctgaatga
     1021 acgcatcaac atggcggtgg tggcactgtt ctacggtgcc ttcgtggtga tggggctgtt
     1081 caacctgttc ctgttctttt ccacccagga ccgcagctac ctgttctatg tgatgtacat
     1141 cgtcaccctg ttcctgtgga catcatccca cgacggcctg ctgcgggagg tgctgctgta
     1201 ccgcaacgac atgctgtcca gctaccgcgt gcattttctg ctgacgctgg tgcccatcct
     1261 gttcggcgcc ctgttctgcc agcgtttcct gcgcacggcg gaaaccatgc cgctgcacca
     1321 ccgcgtgttc agcttcctga tcctgctgtg tctggtggac gcggcgctga ccctggtcag
     1381 tggccgcatc ctgctgccgg attcggtcaa cctgctcacg gtcaccttct ccattgccgg
     1441 cgtatcggcg agcgtgcagg ggttgcggca gggtctgcgc accgcgcgct ttttcctgac
     1501 ggcctgggtg gcggtcatcc ttggctcggt gctgtgggtg ctgacgctgt ccggtgtgct
     1561 gcccttcaat tccttcacgg cgttcagcgt gcacatcggc ggcctgctgg aaaccatcct
     1621 gctgtcactg gcgctggggg accgcatcaa catgctgcag gccgagcgca tccagctgga
     1681 acaggatgcc aaacgcaggc tggaggattc caaccagaaa ctggaggcca gcaaccgctt
     1741 caaggacgag ttcctgtcca ctgtcagcca tgaactgcgc acacccatga acggcatcat
     1801 tggcgcgtcg gaactgctgg aacacaccga cctgaattcc gaacagtaca aatacctctc
     1861 caccatcaac cgctccagcc acgacatgct gggcatgatc gagaacatcc tcacctacac
     1921 ccagttcgaa gccggcaccg ccaccgccgc caaccggccg atgaaaatcc gccagttgct
     1981 ggacgaaatg accgcccact accggggccg cgctgccatc aagcagctgg agttccgcca
     2041 ccacatcgat ccgtcgatcc ccagcgatct ggccggcgat tacgacaagg tgaaactgct
     2101 gctggaccag atcctggata acgccatcaa gttcacggac agcggccata tccgcttcga
     2161 tgtgaccgtg gatgaagagc gcaagcacaa ggacacgctg tggctgaaat tcgtgatcag
     2221 ggacaccggc tgcggcgtgc cggaatcgtt gcagggtgag attttcaatt ccttcaaaca
     2281 gggtgatggc tccatgaccc gacgcaaggg cggcctcggc attggcctgg ccctgagcaa
     2341 gcggatcatc gacatcctgg atggccgcct gacattcaaa tcctcggtac tggacggcac
     2401 gacggtgatc ctggccctgc cattccgccc ctgcgcagcc caggacgaac ccgcccaggc
     2461 ggatctggcc gagagcatca actgcgccag cgtggaaatc ctggtggtgg aagacaatta
     2521 tgtgaacaag ctggtactgg aaggattcct gaaaaaactc ggcttcagcg tgcaggccgt
     2581 ggacaacggc aaggaagccg tggcgctgct ggaaaaacgc gagttcgatc tggtgttcat
     2641 ggactgccag atgccggtga tggacggttt cgaggctacc cgccagctgc gcaagttgcc
     2701 caatcgcaat gccggcattc ccgtggttgc ggtaacggcg aacgccaatc ccggtgaccg
     2761 cgaacgttgc cttgctgccg gcatgaatga ttatctgaaa aagccattca gccagcagtt
     2821 gctggtcagc acgctgaatc gctggctgca gcgccagcgc aatgacagcg tgcaggcctg
     2881 aatcagcgcg gcagtcgaat gcggaaacag gctccgccct gagtggactg atcaatctcc
     2941 agctc
//
LOCUS       DASUEG010000372         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1709214, whole genome shotgun sequence.
ACCESSION   DASUEG010000372 DASUEG010000000
VERSION     DASUEG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709214"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFX11_06890"
     CDS             complement(<1..431)
                     /locus_tag="VFX11_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX4938383.1"
                     /translation="MTWQPTASIDALQARARLNRAIRQFFAVRDVLEVETPLLCQSTA
                     TDPHLQSFAVQAGQNTLYLQTSPEFPMKRLLAAGSGPIYQLCKAFRFEETSRLHNPEF
                     TLLEWYRPGWMLEQLVDEVEALVLDAAAAFNVPMQPFPRFT"
     gene            complement(521..1084)
                     /gene="efp"
                     /locus_tag="VFX11_06895"
     CDS             complement(521..1084)
                     /gene="efp"
                     /locus_tag="VFX11_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4938384.1"
                     /translation="MANYSTNEFRPGLKVLMDGDPCNILENEMVKPGKGQAFNRVRLR
                     NLKTGKIWEKTFKSGDTLEGADIQDQDFQYLYNDGEFYHFMDPKTFEQIGADATAVGD
                     AYKWLKEEDSCIITLWNGVPLAVTPANFVELEIVETDPGLRGDTATGGTKPATLTTGA
                     VVKVPLFVNQGDKIRVDTRTGEYVSRA"
     gene            1168..2187
                     /gene="epmB"
                     /locus_tag="VFX11_06900"
     CDS             1168..2187
                     /gene="epmB"
                     /locus_tag="VFX11_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEX4938385.1"
                     /translation="MITRTAPGWHTEDWQIELSGAVRSVESLWQLLDLPVDSLPAALA
                     AHRDFPVRVPRAFLAKIEKGNPHDPLLKQILPVAAETHSVAGFVADPLAERNANRQSG
                     LLHKYQSRVLLVLGGTCAINCRYCFRRHFPYEDNRLGPEDLAGIQAYLADQPDVNEVI
                     LSGGDPLVTSNARLCALLDLIEAVPHISRLRIHTRLPVVIPQRVDQGLLQRLGQSRLN
                     CVLVTHCNHPQELDTDFDHAMNLLKRQGVTLLNQAVLLRGVNDSVATQVALSERLFAA
                     GVLPYYLHLLDPVAGAHHFDVPESEARTLMQAVHAALPGFLIPRLVREVPGQPGKSLI
                     DLHLT"
     gene            2353..>2637
                     /locus_tag="VFX11_06905"
     CDS             2353..>2637
                     /locus_tag="VFX11_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938386.1"
                     /translation="MSNQADHLSLKLPPHDLEVLSFSAPNPKKIKEWVEGLPQMNVGE
                     CAKRLYAAIQELNRLKTDSETRFQMMETLRGPIHFICKSLEKHFLNKSVIL"
BASE COUNT          545 a          824 c          750 g          518 t
ORIGIN      
        1 tacgtgaatc ggggaaaggg ctgcatcggc acgttgaaag cggcggccgc atccagaacc
       61 agcgcttcta cttcgtccac caactgttcc agcatccagc cgggccggta ccattccagc
      121 agggtgaatt ccggattgtg cagccggctg gtttcctcaa agcggaacgc cttgcatagc
      181 tggtaaatgg gtccgctgcc ggccgcaagc aaccgcttca tgggaaattc cggcgatgtc
      241 tgcagataaa gggtgttctg acctgcctgc acggcaaaac tctgcaggtg cggatcggtg
      301 gccgtactct ggcatagcaa cggagtttcc acttccagca catcacgcac tgcaaaaaat
      361 tggcggattg cccggttgag tcgggcacgg gcctggagag catctattga ggcggtcggt
      421 tgccaggtca tggattcgaa atcgggaaat gttgacgtcc taaacaaaaa aaccggcagc
      481 ctggtccaga ccaccggttt tcttcaatcc aacaatcgca tcacgcccgc gaaacgtact
      541 caccggtccg ggtatccacc cggatcttgt cgccctggtt cacgaacagg ggcaccttca
      601 ccaccgcacc ggtggtcagg gtggcaggct tggtgccgcc ggtggcagtg tcaccacgca
      661 ggcccggatc cgtttccacg atttccagct ccacaaagtt cgccggggtc accgccagag
      721 gcacgccatt ccacagagtg atgatgcagc tgtcttcctc cttcaaccac ttgtacgcgt
      781 cgcccacggc ggtcgcgtca gcaccgatct gctcgaacgt tttcgggtcc atgaagtggt
      841 agaactcgcc atcgttgtac aggtactgga aatcctggtc ctggatatcg gcgccttcca
      901 acgtgtctcc cgacttgaag gtcttttccc agatcttgcc ggtcttcagg ttgcgcaggc
      961 gaacccggtt gaacgcctgt cccttgcccg gctttaccat ctcgttttcg aggatgttgc
     1021 agggatcgcc atccataagg actttaagac ccggacggaa ttcgttggta gaatagttcg
     1081 ccattgaaca ttacctgtac agattgttag caaaaatgga aggccgaaag ccctgatcaa
     1141 accacacccg aaacgggaaa ggcgcacatg ataacgcgaa ctgcccccgg ttggcataca
     1201 gaagactggc aaattgagct gtccggggcc gtgcgcagcg tggaatcgct gtggcagctt
     1261 cttgatctgc cggtagacag cctgcctgcc gccctcgccg cccaccggga ttttccggtg
     1321 cgggtgccgc gtgcgtttct ggccaaaatc gaaaagggca atccgcacga tcccctgctg
     1381 aagcagatcc tgccggtggc ggcggaaacg cactctgtcg cgggcttcgt ggccgatccg
     1441 ctggccgagc gcaacgccaa ccggcagagc ggcctgctgc acaagtacca aagccgggtg
     1501 ctgctggtcc ttggcggcac ctgcgcaatc aactgccgct actgcttccg ccgccatttt
     1561 ccctatgaag acaatcgttt gggcccggag gatctggccg gcatccaggc ctatctggcc
     1621 gatcagccgg acgtgaacga agtgatcctg agcggcggcg atccgctggt gacgagcaat
     1681 gcgcggctgt gcgccctgct ggacctgatc gaagccgtgc cccacatttc ccgcctgcgc
     1741 atccacaccc ggttgccggt ggtgattccg cagcgggtgg accagggcct gttgcagcga
     1801 ctggggcaaa gccgcctcaa ctgcgtgctg gtgactcact gcaatcatcc gcaggaactg
     1861 gacaccgatt tcgatcacgc catgaatctg ctgaaaagac agggggttac cctgctgaac
     1921 caggccgtgc tgctgcgcgg cgtcaatgat tcagtggcga cccaggtggc cctgagcgag
     1981 cggctgttcg ctgccggcgt cctgccatat tacctgcacc tgctggaccc ggtagccggc
     2041 gcccatcatt tcgacgtgcc ggaaagcgag gcccgcaccc tgatgcaggc cgtgcacgcc
     2101 gccctgcccg gcttcctgat accccgcctg gtgcgcgaag tgcccggcca gcccggcaag
     2161 agcctgatcg acctgcattt aacctgacat atcaatcagg tgattcctcc agcacctttc
     2221 cgaacgttgc ctatactgtc tagtaagggc tcgtgccccc atagataccg ggcgaactct
     2281 gacctgacaa cagcagcatg acccgcccgg gactgccata ccggcactac ccgcagtgaa
     2341 caggaatccg gaatgtcgaa tcaggccgac catctcagct tgaagctgcc tccgcatgat
     2401 ctggaggtgc tgtccttcag cgcgcccaat cctaagaaga tcaaggaatg ggtggaaggc
     2461 ctgccacaga tgaacgtggg cgaatgcgcc aaacgcctgt acgccgccat tcaggaactg
     2521 aaccgcctga aaaccgattc cgaaacccgc ttccagatga tggaaaccct gcgcgggccg
     2581 atccatttca tctgcaagag cctggaaaag catttcctca acaagtcggt gatcctg
//
LOCUS       DASUEG010000373         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1278764, whole genome shotgun sequence.
ACCESSION   DASUEG010000373 DASUEG010000000
VERSION     DASUEG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278764"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFX11_06910"
     CDS             <1..390
                     /locus_tag="VFX11_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX4938387.1"
                     /translation="RGRDTMLPARRIPVAARHFANGMPLDDARVSPLYAQLHGLPPLL
                     IHVGDQEVLLHDSTRFADKAREQGVDVTLRIWRDAPHVFQMFAGLVPQGTWSLKEIAQ
                     FMRKQMGLVEENRLRSPALRLAGPSGA"
     gene            394..1437
                     /locus_tag="VFX11_06915"
     CDS             394..1437
                     /locus_tag="VFX11_06915"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4938388.1"
                     /translation="MQVKKFLVTGGAGFIGSHVVRALLAHYDAPVRVLHLPKENLLNL
                     TGLNVELFPGDLTNPADVSAAVEGCDVVFHLAAVYALWLPDMSLMQRVNVDAANNLFR
                     TCHQQGVKRVVYTSSFACFAGQGLDTACTEKSPFNLPESYYSRTKYESHKLAEVWARK
                     GLDVVIVCPTCPLGPGDVGPTPTGRMLVEAFNMPVLLGVNTDSNYIDVRDCALGHLLA
                     LEKGRTGESYILGGENYTHPDIVRRLQRIAGIHRRVVMVEPEWLRPMAKLGVWLAEHV
                     TGKAPFTTEAELVIARKGLVADASKARHELGLQVRPIEETIHDALLWFIEHGYITDSE
                     VINRFHGLQAQAA"
     gene            1497..1572
                     /locus_tag="VFX11_06920"
     tRNA            1497..1572
                     /locus_tag="VFX11_06920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1530..1532,aa:Gly,seq:gcc)
     gene            complement(1638..>2290)
                     /locus_tag="VFX11_06925"
     CDS             complement(1638..>2290)
                     /locus_tag="VFX11_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX4938389.1"
                     /translation="SYNEVNFEQILALQPDLIIGWQTGNSSDALEKLKTLQLPVYLSE
                     PKDLISIARNIRQFGILAGTETVANTAADTFEQKLAQLKADNAQKPVLTVFYQVWEEP
                     LYTLGGGHFSRDMFALCGSRNIFADQEHPSPVVSVEAIVTRNPQVMLTGDHHGERKIE
                     DWKAKWVSWSSIDAVKHDQLYLVDQDIYTRSSPRAVQGAEHLCGILDQARKAYFGK"
BASE COUNT          412 a          644 c          744 g          490 t
ORIGIN      
        1 cgcggccgcg acaccatgct gccggcccgc cgcattccgg tggccgcgcg ccatttcgcc
       61 aacggcatgc cgctggatga cgcccgggtt tcccccctct atgctcaatt gcatggcctg
      121 ccgccgttgc tgattcatgt cggcgaccag gaagtgctgc tgcacgattc cacccggttt
      181 gcggacaagg ctcgcgagca gggcgttgat gtgacgctgc ggatctggcg cgatgcgcct
      241 catgtgttcc agatgttcgc gggactggtg ccgcagggca cctggtcgtt gaaagagatc
      301 gcccaattca tgcgcaagca gatgggcctt gtggaagaaa accggttgcg gtcgccggcg
      361 ttgcgcctgg ccgggccgtc tggagcctga atcatgcaag tgaaaaaatt cctggtcacc
      421 ggcggtgccg gttttattgg ttcccatgta gtgcgagcac tgctggcaca ttacgacgcg
      481 ccggtgcggg tgctgcatct gcccaaggaa aacctgctca acctgacggg gctgaacgtg
      541 gagctgttcc ccggcgacct gacgaaccct gcagatgtaa gcgcggcggt ggaggggtgc
      601 gatgtggtgt tccatctggc cgccgtgtat gcgttgtggc tgccggacat gtcgctgatg
      661 cagcgggtga acgtggacgc cgccaacaac ctgttccgca cctgtcatca gcagggtgtg
      721 aagcgggtgg tgtacaccag ttccttcgcc tgctttgccg ggcagggact ggatacggcc
      781 tgcaccgaga agtcaccgtt caatctgccg gaaagctatt attcccgcac caagtacgaa
      841 tcccacaagt tggcggaagt ctgggcacgc aagggcctgg atgtggtgat tgtgtgcccg
      901 acctgtccgc tgggccccgg tgatgtgggc ccgaccccga cgggtcgcat gctggtggag
      961 gcgttcaaca tgccagtgct gctgggcgtg aataccgaca gcaactacat cgacgtgcgc
     1021 gattgtgccc tgggccatct gctggcgctg gaaaagggcc gtactggcga gagctatatt
     1081 ctgggcggcg agaattacac ccacccggac atcgtgcgcc ggctgcagcg gattgcgggt
     1141 atccatcgcc gcgtggtgat ggtcgagccg gaatggctgc ggccgatggc caaactgggc
     1201 gtgtggctgg cggagcatgt gacaggcaag gcgccgttca ccaccgaggc ggaactggtg
     1261 attgcccgca agggtctggt ggcagatgcc agcaaggcgc ggcatgaact gggcctgcag
     1321 gtgcggccga ttgaggaaac cattcatgat gcgctgttat ggtttatcga acacggctat
     1381 atcactgatt cggaagtgat taaccggttt catggattac aggcgcaggc cgcctgagtt
     1441 tgaaaaaagg tgttgacgga cggttgggtg ccccttagaa ttcgcgccca caccaagcgg
     1501 gaatagctca gttggtagag cacgaccttg ccaaggtcgg ggtcgcgagt tcgagtctcg
     1561 tttcccgctc caaaacatta aaaaggcctg acggaaacgt cgggcctttt tggtttatat
     1621 cgcgaccccg gcttttatca cttcccgaaa taggccttgc gggcctgatc cagaatcccg
     1681 cacagatgct cggcaccctg cacggcccgg ggtgaggagc gggtgtagat gtcctggtct
     1741 accagataca gctggtcgtg cttcacggca tcgatgctgg accagctcac ccacttcgct
     1801 ttccagtcct cgatcttgcg ttcgccatga tggtcgccgg tgagcattac ctgcgggttg
     1861 cgggtgacga tggcttccac cgacaccacc ggtgagggat gttcctggtc ggcaaagata
     1921 ttgcggctgc cacacagcgc aaacatgtcg cggctgaagt ggccgccgcc cagggtgtag
     1981 agcggttctt cccacacctg ataaaacacg gtcagcaccg gcttttgcgc gttgtcggcc
     2041 ttgagctgtg ccagtttctg ttcgaaggtg tcggcggcgg tattggcgac ggtttcggtg
     2101 cccgccagga tgccgaactg gcggatattg cgggcaatcg agatcaggtc tttcggttcg
     2161 cttaaataca ccggcagttg cagggttttc agtttctcca gggcgtcgct gctgttgccg
     2221 gtctgccagc cgatgatcag gtccggttgc agcgccagga tctgttcaaa gttcacttcg
     2281 ttgtagctgc
//
LOCUS       DASUEG010000374         3660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1748789, whole genome shotgun sequence.
ACCESSION   DASUEG010000374 DASUEG010000000
VERSION     DASUEG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748789"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1472
                     /locus_tag="VFX11_06930"
     CDS             312..1472
                     /locus_tag="VFX11_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEX4938390.1"
                     /translation="MNMNKPPALFQQIQADSLGIQSRSTFNPVQMWACLGGLILAIQI
                     YIWAKWITGPYFVRVPPGPSDPPLFMKTILVIWTTVICIGLPIGIYYFIIRPWRQERR
                     ITTDGMLLVSCGLLFFQDPLLNYFNTWSTYNTWMWNRGSWVKDIPGWVSYGEPGRMMA
                     EPLLMNAPGYSYGVLLCTILGCWIMRRAKMRWPGISNLGLIGVLIAWTFFFDLIIEGL
                     FLMPMGLFTYPGAIQSLSINAGTYYQWPLYEGLMWGGVQAGLCSLRYFTDDRGRTFVE
                     RGLERVQGGFAKQQMVRFLAIFAACSLFFFVCYNLPAQWLAMHADPWPEDIQKRSYFT
                     MGICGEGTGRLCPHPALPIQGQNSAYIDPDGKVVFPAGVELPKIIPYDRGEK"
     gene            1474..2292
                     /locus_tag="VFX11_06935"
     CDS             1474..2292
                     /locus_tag="VFX11_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX4938391.1"
                     /translation="MTSAEKIPARSAVDAPFYRPVNDEVEVFRAAARRGLPVLLKGPT
                     GCGKTRFVEAMAHELGRELITVAGHEDLTSADLVGRFLLKGGETVWVDGPLTRAVREG
                     AICYLDEIVEARQDTTVVIHPLADHRRVLPIDRLGTILEAAPGFQLVISYNSGYQSVL
                     KSIKESTRQRFVAIEFDFQPANIETEVVAHEAGVDFETARTLVTIGGAIRKLSGAQLR
                     EAASTRMLILAGGLVAEGLSLRSAVRSAIVQALSDDPDIVRALGELVDAVIPRS"
     gene            2289..>3660
                     /locus_tag="VFX11_06940"
     CDS             2289..>3660
                     /locus_tag="VFX11_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4938392.1"
                     /translation="MSTAGMASADMKSAETESPDRFRFLASFLAGRSLGVVSAADGEA
                     AFTDGQQVFVSAGGSVEEQRQEVLLQCALLGAGSLDHERVKSLRGRPQIARRYLALEG
                     QRVLAELATCLPLAAALRPDREPATASADESLALARSRKTIADPPPWFGVIRPSRLLA
                     PAAGPGAQPTPKDLRLQFDLTDVPEAENEDDDEQTEPSKILKLFENPLFSSNRASDFF
                     RKLFSMSRSSSDGAAGGEMQVRTIRRVSSLGANAKPLPVPIHFTSSDTPGAAAGVGGA
                     LYPEWDVHNNQYRPEWCRVIDFPLLSTPDLAAAAVQQDEVLRRRLTRLGLGPKVIRGQ
                     PDGEDLDIEALINLCIDLKTGHSPHEHIYLERRKLGRNLGVLILLDASGSATDTDPEG
                     LSVHDHQRRAAATLAVTLEELGDRVAVYGFRSHGRSNVHLLGLKPFTQRFGAGGRARL
                     NQLQPDG"
BASE COUNT          696 a         1064 c         1116 g          784 t
ORIGIN      
        1 gcgaaacctg ggccgcactc agtagcgaat tgaatttgca gcagatggtg gatctcacct
       61 tcacgatcgg cagctatgtg ctggcgggaa tgtacatgaa ttgctttgct ctgccactgc
      121 agaaaaacca gaagggattc gaagccttcc agtaaattgt tttcgacaga gtgaatggtt
      181 tcctgcaaat aaaatttttc atgccgtaag tttttagaaa aatagtccct tgggaggatg
      241 tcgcagttcc cgccgctgat aacatcagca tacggaaaat tcaagaagaa gaaacgaagg
      301 agcggaaacg catgaacatg aacaaacctc cagcgctgtt tcaacagata caggccgatt
      361 cactcggtat ccaatcccgc tccaccttca atcccgttca gatgtgggcc tgtttgggtg
      421 gcctgattct cgccattcag atttatatct gggccaagtg gatcaccggt ccctattttg
      481 tgcgggtgcc ccccgggccc agcgatccgc cgctgttcat gaaaaccatc ctcgtcatct
      541 ggacgacggt catctgtatt ggtcttccca tcggcatcta ttacttcatc atccggccct
      601 ggcggcagga gcggcgcatc accaccgacg gcatgctgct ggtgtcttgc ggtctgctct
      661 ttttccagga cccgctgctg aattacttca acacctggag tacctacaac acctggatgt
      721 ggaatcgcgg ttcatgggtg aaagacattc cgggctgggt atcctatggc gaaccagggc
      781 gcatgatggc ggagccgctg ctgatgaatg cgccgggtta ttcctacggc gtgctgctgt
      841 gcacgatact gggatgctgg atcatgcggc gtgcgaagat gcgttggccg ggcatcagca
      901 acctgggcct gatcggtgta ttgatcgcgt ggacgttttt cttcgatctg atcatcgaag
      961 gcctgttcct gatgccgatg ggacttttca cctatcccgg cgcgatccag tcgctgtcga
     1021 tcaacgcagg cacttattac cagtggccgc tttatgaagg cttgatgtgg ggtggtgtgc
     1081 aggccgggct ctgctcgctg cgctatttca ctgatgatcg cggccgcacg tttgtcgaac
     1141 gtggtcttga acgggttcag gggggattcg caaaacaaca gatggtccgc ttcctggcga
     1201 tcttcgcggc gtgcagcctg tttttcttcg tctgctacaa cctgcctgcg cagtggcttg
     1261 cgatgcatgc cgatccctgg ccggaagaca tccagaagcg ttcctatttc accatgggta
     1321 tctgcggtga aggcacggga agactgtgcc cccatcctgc gcttcccatc cagggccaga
     1381 attccgccta catagatccg gatggcaagg tggtctttcc cgcaggtgtg gagctgccga
     1441 aaataattcc ctatgaccgc ggagagaagt gacatgacca gtgctgaaaa aattcctgcg
     1501 cgttctgcgg tggatgcgcc gttttaccgc ccggtcaacg atgaggtgga agtatttcgc
     1561 gcggcggccc gtcgcggttt gccggtgttg ctgaaaggcc cgaccggatg cggcaaaacc
     1621 cgtttcgtgg aggcgatggc ccatgagctg ggccgcgaac tgatcacggt ggccggtcat
     1681 gaggatctga cctcggcgga tctggtgggg cgttttctgc tgaaaggtgg cgagacggtc
     1741 tgggtggacg ggccattgac ccgcgcggtg cgcgaaggtg cgatctgcta tctggatgaa
     1801 atagtggagg cgcgtcagga caccacggtg gttattcatc cgctcgccga tcatcgtcgc
     1861 gtgctgccca tcgaccggct cggcaccatt ctggaagcgg cgcccggatt ccagttggtg
     1921 atttcctata actcgggtta tcagagcgtg ctcaagagca tcaaggaatc aacccgccag
     1981 cgtttcgtgg cgatcgagtt tgatttccag cccgccaata tcgagaccga agtggtggcc
     2041 cacgaagcag gtgtcgattt tgaaaccgcg cggacgctgg tcactattgg cggcgccatc
     2101 cggaaactga gtggcgcaca gttgcgggaa gcggcgtcca cccggatgct gatactggcc
     2161 ggcgggcttg ttgcggaagg gctcagtctg cgcagtgcgg taaggtcggc catcgtgcag
     2221 gcgctgtcgg atgatcctga tattgtccgc gcactcggcg aactggtcga tgcggtaata
     2281 ccccggtcat gagtactgct ggcatggcga gcgccgacat gaagagcgct gaaacagaga
     2341 gccccgaccg gtttcgcttt ctcgcctcct ttctcgcggg tcgttccctt ggcgtcgtta
     2401 gcgcagcgga cggagaagcc gcatttaccg acgggcaaca ggtgttcgtc tcggcgggcg
     2461 gttcagtcga ggaacagcgc caggaggtgc ttctgcaatg cgctctgctc ggcgccggga
     2521 gtctcgatca cgaacgcgta aaatcattgc gcggacgtcc gcagatcgcg cgccgctatc
     2581 tggcactgga agggcagcgg gtgctggctg aacttgccac gtgcttgccg ctggccgctg
     2641 cgttgcgccc cgaccgggag cccgcaacag cctcggcgga cgaatcgctg gcgctggcca
     2701 gaagccgcaa gactatcgcc gatcctcccc cgtggttcgg cgtcatccgg ccttcgcgat
     2761 tgttggcgcc ggccgccggg cccggtgcac aacccacgcc aaaagatctc cgcctgcagt
     2821 tcgatctgac cgatgtgccc gaagctgaaa acgaggatga cgacgaacaa accgagccca
     2881 gcaaaatcct caagctgttt gaaaatccgc tgttcagctc gaaccgcgcg tcggatttct
     2941 tccgcaagct gttcagcatg tcgcgctcat ccagtgatgg cgcggcgggt ggcgagatgc
     3001 aggtgcggac catccggcgg gtcagcagcc tgggcgcaaa tgccaagccg ctgccggtac
     3061 cgattcactt caccagcagc gacacgcccg gcgctgccgc cggcgtgggt ggtgcgctgt
     3121 atccggagtg ggacgtacac aacaaccagt accggccgga gtggtgccgc gtcatcgatt
     3181 ttccgctgct gtcgacgccg gatcttgctg ctgctgcggt tcagcaagac gaggtactgc
     3241 gtcgacgcct gacccggctc ggtctcggtc ccaaggtaat tcgcggtcag ccggatggcg
     3301 aggatctcga catcgaggca ctcatcaatc tgtgcatcga tctgaaaacc ggccactcgc
     3361 cgcacgaaca catttatctg gagcggcgca aactgggccg caatctgggt gtgcttatcc
     3421 tgctcgacgc ctccggctcc gcgacggata ccgatccgga aggtctctcg gtgcacgatc
     3481 atcaacgacg tgccgccgcc accctggcgg tcacgctgga agaactgggc gatcgcgtcg
     3541 ccgtttacgg tttccgttcc cacgggcgtt ccaacgtcca cctgctcggg ctcaaaccct
     3601 tcacgcagcg tttcggcgcc ggcggacgcg cccgccttaa ccagctgcag ccggacggtt
//
LOCUS       DASUEG010000375         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2089086, whole genome shotgun sequence.
ACCESSION   DASUEG010000375 DASUEG010000000
VERSION     DASUEG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089086"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFX11_06945"
     CDS             <1..1101
                     /locus_tag="VFX11_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX4938393.1"
                     /translation="GTGLYTPQQTISNAELVKSFNTYVDNFNAENRAAIEAGTLEALS
                     HSSEEFIVKASGIESRYVIDKTGVLDPARLCPRIPERADEEPSIQCEIAVAAARDALA
                     QARKTVDEVDAVIVACSNLQRAYPAMAIEVQNALGIKGFGFDMNVACSSATFGLQCAR
                     DAVLTGSARCVLVLNPEICSGHLAWQDRDCHFIFGDVCTALVVERVETCKNPDAWEIL
                     GTRLQTQFSNNIRNNFGFLNRCDESGIGARDKLFCQNGRKVFKEVVPMVAELIASHLS
                     DESIEVSGLRRMWLHQANLGMNQLISKKVLGRDAEPAEAPVILNEYANTSSAGSIIAF
                     HKHRQDLQSGEVGVICSFGAGYSAGSVIVKRL"
     gene            1394..1768
                     /locus_tag="VFX11_06950"
     CDS             1394..1768
                     /locus_tag="VFX11_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938394.1"
                     /translation="MAKFNSLKPVAAGLGLAVAASALSLSTAVNAAQNPFATAELSGG
                     YMLAADDTKTGEGKCGEGKCGGSMKTEEGKCGGEKAKEEGKCGEGKCGSEDARAKAAA
                     EGKCGEGKCGGDKAKAEGKCGK"
     gene            1787..>2073
                     /locus_tag="VFX11_06955"
     CDS             1787..>2073
                     /locus_tag="VFX11_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX4938395.1"
                     /translation="MNQHKTSVIRGAGLGLRRAFIGPLCERTSNRPDSDFLAGIDFME
                     VAPENWISVGGRFGKQFRALTEVCPFVLHGLSLSLGSPAPLDLALVRNIKG"
BASE COUNT          438 a          592 c          614 g          429 t
ORIGIN      
        1 ggtaccgggt tgtacacccc tcagcaaacc atcagcaacg ccgaactcgt caagtcgttc
       61 aacacctatg ttgacaactt caatgcagaa aaccgcgccg ccatcgaggc gggtacgctg
      121 gaagcgctga gccattccag cgaggaattc attgtcaagg catccggcat cgaaagccgt
      181 tacgtgatcg acaagaccgg cgtgctggac ccggcacgtc tgtgtccccg cattcccgaa
      241 cgcgctgacg aagaaccctc catccagtgt gaaatcgctg tcgccgcggc gcgtgatgca
      301 ctggcccagg cccgcaagac ggtggacgag gtcgatgcag tcatcgtcgc ctgttccaac
      361 ctgcagcggg cctatcctgc gatggccatc gaagtgcaga acgcgctggg catcaagggt
      421 ttcggtttcg acatgaacgt cgcctgttcc tccgccacct tcggattgca gtgcgcccgc
      481 gatgccgtgc tcaccggttc cgcccgttgc gtgctggtac tgaatccgga aatctgctcc
      541 ggccatctgg cctggcagga ccgcgactgt catttcattt tcggcgatgt gtgcacggcg
      601 ctggtggtgg aacgggtgga aacctgcaaa aaccccgacg cctgggaaat tctcggcacc
      661 cgcctgcaaa cccagttttc caataatatc cgcaacaatt ttggtttcct gaaccgttgc
      721 gacgaaagcg gcatcggcgc ccgcgacaag ctgttctgcc agaatggacg caaggtattc
      781 aaggaagtgg tgcccatggt ggcggagctg attgccagcc atctgtcaga cgaatccatt
      841 gaagtgagtg gattgcgccg catgtggctg caccaggcca acctgggcat gaaccagctg
      901 atttcgaaga aagtgctggg ccgcgatgcc gagccggccg aggcgccggt gatcctgaat
      961 gaatatgcga ataccagttc ggccgggtcc attatcgcgt ttcacaagca ccgccaggat
     1021 ctgcaatccg gtgaagtggg tgtgatctgt tccttcggcg ccggatattc tgccggcagc
     1081 gtcattgtca agcggctgta atctgaatag ccgtccaatc tgtccatgga aggagccgcc
     1141 cggctccttc ctgcttttac cacgccagct ttgtccaatt gatctcgcct gtatggtctg
     1201 ctactttcgg gtggccacgc cggcgtcttc ggtgttgtga aaaattacca ccagattccg
     1261 gcgcctgcct gtaacaaatg ccggaatcaa tcgtctcatc tgcagttgcg acaatgttgt
     1321 ctggttgtcg ccttcccctt gcggacactg atgtccgttt ttttaatcaa ccggtacttg
     1381 aggagaaaca cccatggcaa agttcaattc actgaaaccc gtcgctgctg gtctgggcct
     1441 ggcggttgct gcatcggcat tgagtttgtc cactgctgtc aacgcggcac agaatccgtt
     1501 cgccaccgct gaactgtctg gcggctacat gctggctgcg gatgacacca agacaggcga
     1561 aggcaaatgc ggtgagggca agtgtggcgg ttccatgaaa acggaagaag gtaaatgcgg
     1621 aggcgaaaaa gccaaggaag agggcaagtg tggcgaaggc aagtgcggca gtgaagatgc
     1681 ccgcgccaaa gcggcagccg aaggcaaatg cggcgaaggc aagtgcggtg gagacaaggc
     1741 caaagccgaa ggcaagtgcg gcaaataaat ccggagccat tgcaacatga accagcataa
     1801 gacctccgtt attcgtggcg cgggtctggg tttgcggcgg gccttcatcg gcccgctttg
     1861 cgaacgcacg tccaatcgtc cggatagcga tttcctggcc ggaatcgatt tcatggaagt
     1921 cgcccccgaa aactggatca gcgtcggcgg acgcttcgga aagcagttcc gcgcactcac
     1981 cgaggtctgt cccttcgtgc tgcatggcct gtcgttgtcg ctgggcagcc cggcgccgct
     2041 ggatctggcg ttggtgcgca acatcaaggg att
//
LOCUS       DASUEG010000376         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1366266, whole genome shotgun sequence.
ACCESSION   DASUEG010000376 DASUEG010000000
VERSION     DASUEG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366266"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="VFX11_06960"
     CDS             <1..2185
                     /locus_tag="VFX11_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4938396.1"
                     /translation="VLGIVVDEAIVIGENIFSYRRKGLNGVEAAIRGAQELFFPMLAS
                     VLTTICAFLPLLLLPGPEGKLMQVIPLVIIFTLLISVIEASLMLPAHLSGSKPDLYDS
                     FPVVGKVQKWISEHLDWFLEHRYRPFLEVCLNWRYSVVTAFVSVLLVTIGIVAFGWLK
                     VVMFSQIEGDTASATIRFSENTPRVVTDSGIKQLEKAALEIMRDFRDPDGSPQILNVF
                     TSYQPDGGGHIVMEFQASENRHFSGQDVVAEWRKRAGDIPEMADLDFKSTLLASGTMI
                     DIELTSNDNDDLQRAAKALKARLSEFEGLYAVRDSLQTAKQELMINLKPTGRNLGLSL
                     EQVAAQVRQAYHGLSLQNINRADGDVAVVLRYSEADRNSLWSLENMNLVLPTGKTVPL
                     SSVADIDYGEGASEIKHHQRHRVVRVSAKVDDAATSIGKVMTVLKADFLDRLSLDYPD
                     MRWAVAGAQKDRDEFIDFLSKAYLMALLGMYTLMAFQFRSYGQPLMVMIAIPFGIIGA
                     LAGHLIVGIDVTIWSLIGIVAVSGVVVNDNLVLIDYINDCRRSGVPLQQAIRDAGVQR
                     ARAVILISLTTLVGVLPMIMEKSLQAQFMIPMAVSLGFGTLFSSTISLILVPSMYRIS
                     VDIGELLGRLFSLGGRSAPEQAELQPALAVAGAGGSEAVPEVAAAAPAGDGRTEWHVG
                     LDEAYELGYKAALEGQPRQAEFELEVLAASWEAGWDDGAEEARRS"
     gene            2227..>2411
                     /locus_tag="VFX11_06965"
     CDS             2227..>2411
                     /locus_tag="VFX11_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578595.1"
                     /note="member of the SPOUT superfamily of
                     methyltransferases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HEX4938397.1"
                     /translation="MLHFVLVEPAVPENVGAAARALKTMGYTSLWIVNSGVHLEKPAR
                     ILAHGSTDILDQVRTFP"
BASE COUNT          421 a          716 c          794 g          480 t
ORIGIN      
        1 ggtgctgggc atcgtggtgg acgaggcgat cgtgatcggc gagaacatat tttcataccg
       61 gcgcaagggc ctgaatggcg ttgaagccgc cattcgcggt gcccaggaac tgtttttccc
      121 gatgctggca tcggtgctca ccaccatctg cgccttcctg ccgctgctgc tgctgcccgg
      181 gcccgagggc aagctgatgc aggtgattcc gctggtgatc atcttcacgc tgctgatctc
      241 ggtgatcgag gcgtcgctga tgctgccggc tcacctgtcc ggttcaaagc cagacctgta
      301 cgacagtttc cccgtggtgg gcaaggtgca gaaatggatt tccgaacacc tggactggtt
      361 tctggaacac cgttaccggc cgttcctgga agtctgtctg aactggcgct attcggtggt
      421 gacggcgttc gtcagcgtgc tgctggtgac catcggcatc gttgctttcg gctggctgaa
      481 ggtcgtgatg ttttcccaga tcgagggtga caccgccagc gccaccatcc gtttttccga
      541 aaacacgccg cgtgtggtaa cggattccgg aatcaagcag ctggaaaagg ccgcgctgga
      601 aatcatgcgg gatttccgtg atcccgatgg cagcccccag atcctcaacg tgttcacctc
      661 ctatcaaccg gatggcggcg gtcacatcgt gatggaattc caggccagcg agaaccggca
      721 cttctccggg caggatgtgg tggccgagtg gcgcaagcgt gccggtgaca ttccggaaat
      781 ggcggatctg gatttcaagt ccacgctgct ggcgtcgggc accatgatcg atatcgagct
      841 gacctcgaac gacaacgatg acctgcagcg ggcggccaag gcgctgaagg cgcggctgtc
      901 ggaattcgag gggctgtatg ccgtgcgcga ttccctgcag actgccaagc aggaactgat
      961 gatcaacctc aagcccaccg gccgcaacct ggggctgtcg ctggagcagg tggcggcgca
     1021 ggtgcgtcag gcgtaccacg gcctcagcct gcagaacatc aaccgcgccg atggcgatgt
     1081 ggcggtggtg ctgcgctatt ccgaggccga ccgcaattcc ctgtggagcc tggagaacat
     1141 gaatctggtg ctgcccaccg gcaagacggt gccgctgtcc agcgtcgcgg atatcgatta
     1201 cggcgagggc gcctcggaaa tcaagcacca ccagcggcac cgtgtggtgc gggtgagcgc
     1261 gaaggtggat gatgccgcca cctcgatcgg caaggtcatg accgtgctga aagcggattt
     1321 cctcgaccgc ctgagtctgg actatcccga catgcgctgg gccgtggccg gtgcccagaa
     1381 ggaccgcgac gagttcatcg actttctgtc caaggcctac ctgatggcgc tgctgggcat
     1441 gtacaccctg atggcgttcc agttccgttc ctacggtcag ccgctgatgg tgatgatcgc
     1501 gattccgttc ggcatcatcg gtgccctggc gggccacctg atcgtcggta ttgatgtcac
     1561 catctggtcc ctgatcggca tcgtcgccgt gagcggggtg gtggtgaacg acaacctggt
     1621 gctgatcgat tacatcaacg attgccggcg cagcggtgtg ccgctgcagc aggccatccg
     1681 cgacgccggc gtgcagcggg cccgcgccgt cattctgatc tcgctcacca cgctggtggg
     1741 tgtgttgccc atgatcatgg aaaagagcct gcaggcgcag ttcatgatcc cgatggcggt
     1801 gtcgctgggt tttggcaccc tgttctccag taccatcagc ctgatcctgg tgccgtcgat
     1861 gtaccggatc tcggtggata tcggtgaact gctgggccgg ttgttcagcc tgggtggccg
     1921 cagtgcgccc gaacaggccg aactgcaacc ggcattggcg gtggccggag ctggcggcag
     1981 tgaagcagtg cccgaagtgg cggcggctgc gcctgccggc gacggtcgca ccgaatggca
     2041 cgtgggcctg gatgaagctt acgagctggg atacaaggcg gcgctggaag gtcagccccg
     2101 tcaggccgag ttcgaactgg aggtgctggc cgccagctgg gaagcgggct gggatgacgg
     2161 cgccgaggaa gcgcggcgtt cctgacgggc tccgcgccac tgtcttgaac aagggagggc
     2221 tcatccatgc tgcatttcgt gctggttgag ccggcggtgc cggaaaacgt cggtgccgcc
     2281 gcccgagccc tcaagaccat gggctacacc agcctgtgga tcgtcaattc cggcgttcat
     2341 cttgaaaagc ccgcccgtat ccttgcccac gggtccaccg acattctcga ccaggtgcgc
     2401 acctttcccg a
//
LOCUS       DASUEG010000377         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_590554, whole genome shotgun sequence.
ACCESSION   DASUEG010000377 DASUEG010000000
VERSION     DASUEG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590554"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFX11_06970"
     CDS             complement(<1..937)
                     /locus_tag="VFX11_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX4938398.1"
                     /translation="MKANEIAELLDEPACSHNKKEKSGCSKPKPGATDGGCAFDGAQI
                     ALLPIADVAHIVHGPIACAGSSWDNRGTRSSGPELYRIGMTTDLTEQDIIMGRAEKRL
                     FHAIKQAVEDYAPPAIFVYNTCVPALIGDDIDAICKSATERWNVPVVPVDAAGFYGTK
                     NLGNRIAGEAMVKYVVGTREPDPLPESSERPGIRVHDVNLVGEYNIAGEFWYVLPLLD
                     ELGLRVLCTLSGDARFREVQSMHRAEVNMMVCSKAMLNIARKLQERYGTPWFEGSFYG
                     ITDTSQSLRDFARLINDPDLTARTEALIEREEKRIR"
     gene            complement(1163..1906)
                     /locus_tag="VFX11_06975"
     CDS             complement(1163..1906)
                     /locus_tag="VFX11_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HEX4938399.1"
                     /translation="MTPMADCRVCRYRQNALLAGRCAPGDACVVVDSGRQIDRFFRAN
                     PFLAMNYLADPFWERRAIAVRYSPVETLAQLINDPDEAVRRAVAYRLPREQLTALMND
                     EDREVRITVADRLPLEKLELMANDPDYLVRAYVAQRLPVGRLFRLIRDPDRQVRKTVA
                     KRLPTESLGLMCGDSEPEVRRILASRLQGDDVVELLFDADWTVRLAAVENAPLDVLAG
                     IRNEADPEVLNAIALRLQGEEPSVGSAPE"
     gene            complement(2024..2290)
                     /locus_tag="VFX11_06980"
     CDS             complement(2024..2290)
                     /locus_tag="VFX11_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HEX4938400.1"
                     /translation="MIAAEELDAVVQTVEQSGLSEAVLTQLRQQFPGKHFTWCMDDDI
                     NNGRPVASREHFAVYLVNSSDHCSVLTQDMGSASGYVLAEIIAD"
     gene            complement(2336..3043)
                     /locus_tag="VFX11_06985"
     CDS             complement(2336..3043)
                     /locus_tag="VFX11_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX4938401.1"
                     /translation="MAAPLEDDIALRIGLAARVLPGADARNLLRMLIRILGEPINHAR
                     LQRLRAAPLRAAIGKQFRAVNAEQFQQAFALLKGQGVQRVTVPGPVIETGVYCELSGS
                     VRVACASGSGERIDGHFGNCPRFLIYQVAPDYIRLIDIREPAPTQHSNSQDADRNQQR
                     AALIQDCAVLYTLSIGGPAAAKAVRAGLHPVKLAEAVPARDELLRLQAVLARDNPPPW
                     LVKAMGAQPRPRPLAAE"
     gene            complement(3046..3267)
                     /gene="nifT"
                     /locus_tag="VFX11_06990"
     CDS             complement(3046..3267)
                     /gene="nifT"
                     /locus_tag="VFX11_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HEX4938402.1"
                     /translation="MPSVMIRRNSNGQLTLYVAKRDLEENIVSIEFDSPERWGGEVVL
                     ADGSSYHIDPIAPPHLPVTVRAKRAGGAD"
BASE COUNT          677 a         1086 c          945 g          660 t
ORIGIN      
        1 cacggatgcg cttttcctcg cgttcgatca gcgcttcggt acgcgctgta agatcaggat
       61 cattgatcag gcgggcaaaa tcccgcagcg actgcgaggt gtcggtgatg ccgtaaaaac
      121 tgccttcaaa ccagggtgtg ccgtaacgct cctgcagctt gcgggcaatg ttgagcatgg
      181 ccttggagca caccatcatg ttcacttcgg cgcggtgcat ggattgcact tcgcgaaagc
      241 gcgcatcgcc ggacagcgtg cacagtaccc gcaaacccag ttcatccagc agcggcaaca
      301 cgtaccagaa ttcaccggca atgttgtatt cacccaccag attgacatcg tgcacacgga
      361 tgccggggcg ttcactggat tctggcagcg gatcaggctc acgggtgcct accacgtatt
      421 tcaccatggc ttcaccggca atacggttgc ccaggttttt ggtgccgtaa aagccggcgg
      481 catccaccgg caccaccggc acgttccagc gctcggtggc ggatttgcag atggcgtcga
      541 tgtcatcgcc aatcagcgcg ggtacacagg tgttgtaaac aaagatcgcc ggcggcgcgt
      601 aatcttccac cgcctgcttg atggcatgga acagacgctt ttccgcgcgt cccatgatga
      661 tgtcctgttc cgtcagatcc gtggtcatgc cgatacggta caattccgga ccgctggagc
      721 gggtgccgcg gttgtcccag gaactgccgg cacaggcgat ggggccgtgc acaatatggg
      781 caacatcggc aatcggcagc aacgcaatct gcgcgccgtc aaaggcacag ccgccgtcag
      841 tggcaccggg cttgggtttc gagcaaccgg atttttcctt cttgttgtga ctgcaggccg
      901 gttcgtcgag cagttcggca atttcgtttg ctttcatctg tgactcctat cggtctacct
      961 atcccggtgc gtgtgcatga ataaccaaag caattccagt tggaacgttg caaacgggtc
     1021 attgcaaagc gcattccagc catgcaatac gccgccagcc cgtgctggcg cggctttttt
     1081 ggggtggccg cttgacagct actgtcacaa acccgacaaa agccgattca caccgcgaaa
     1141 gcgatcgcac ctttgcgaca attcattcgg gcgccgaacc gacagaaggc tcttccccct
     1201 gcaaacgcag ggcaatggca ttgagcacct caggatcggc ttcgttgcgg attcccgcca
     1261 gcacgtccag cggcgcgttt tccactgcgg ccagacgcac ggtccagtcc gcatcgaaca
     1321 gcagttccac cacgtcgtcg ccctgcagac gtgaggccag gatgcgacgc acttccggct
     1381 cggaatcccc acacatcagg ccgagacttt ctgtcggcag acgtttggcc accgtcttgc
     1441 gcacctggcg gtccggatcg cggatcagcc ggaataaccg ccccaccggc aagcgctgcg
     1501 ccacatacgc ccgcaccagg taatcaggat cgtttgccat caattcgagt ttttccagcg
     1561 gcagacggtc cgccaccgta atgcgtactt cgcggtcctc gtcgttcatc agcgccgtca
     1621 actgttcccg tggcaaccgg tatgccaccg cgcgccgcac tgcctcgtcg ggatcgttga
     1681 tcagctgcgc cagcgtttcc accggcgaat agcgcacggc aattgcgcgc cgctcccaaa
     1741 agggatcggc cagatagttc attgccagaa atggattggc acgaaagaag cggtcgatct
     1801 ggcgcccgct gtcgactacg acgcaggcat cgcccggcgc acaacgtccc gccagcaacg
     1861 cattctgccg gtaacggcag acacggcaat ccgccatcgg cgtcatgtca ctggcggcat
     1921 cgacaaactc ggcatcggtg ggatcgctac cggaagaatt cttgctgctg tccatgaaaa
     1981 aatcctgtct gatcgtttct ggccgagatc ctgtgcaagc ccatcagtcc gcaatgattt
     2041 ccgccagaac gtagcccgag gcactgccca tgtcctgcgt gagcacggaa cagtgatcgc
     2101 tggaattaac cagatacaca gcaaaatgtt cgcgggacgc caccggccgg ccgttattga
     2161 tgtcgtcatc catgcaccag gtgaaatgtt tgcccggaaa ctgttgccgc aattgcgtca
     2221 gcaccgcttc cgacaaaccg ctttgctcca cggtctgcac caccgcatcc agttcttctg
     2281 ctgcaatcat gttgtttcct ccacttacat tttctttcac acaacagcgc ccatttcact
     2341 ctgccgccag cggacgcggt cgcggttgcg cgcccatcgc cttcaccagc cagggcggcg
     2401 gattgtcgcg tgccagcacg gcctgcagcc gcaacaattc atcacgggca ggcacggctt
     2461 ctgccagttt caccggatgc aggccggcac gcaccgcctt ggccgccgcc ggtccgccga
     2521 tggaaagcgt gtaaagcacc gcgcaatcct gaatcagcgc ggcgcgctgc tggttgcgat
     2581 cggcatcctg gctgttgctg tgctgcgtcg gcgccggttc gcggatatcg attaaccgaa
     2641 tgtaatccgg cgccacctgg taaatcagaa agcgcggaca attgccgaaa tgaccgtcga
     2701 tgcgttcgcc gctacccgac gcgcaggcca cccgcaccga cccggacagt tcacagtaaa
     2761 cgccggtttc aatcaccggc ccgggcacgg tcacccgctg cacgccctgc cctttcagca
     2821 gcgcaaatgc ctgctggaat tgctccgcgt tcacggcacg aaactgtttg ccaatggcag
     2881 cccgcaacgg cgccgcccgc aagcgctgca agcgcgcgtg attgatgggt tcccccagaa
     2941 tgcggatcag catgcgcaac agattgcgcg cgtccgcacc cggcaatacg cgcgcagcca
     3001 gtccgattcg cagcgcgata tcgtcctcca gcggcgcggc catggtcagt cggctccgcc
     3061 cgcccgcttg gcgcgcacgg tcaccggcaa atgcggcggc gcgatggggt cgatgtggta
     3121 ggacgatccg tccgccagca cgacttcacc gccccagcgc tccggggaat cgaattcgat
     3181 ggaaacaata ttttcttcca gatcgcgttt ggcgacgtac agggtcaact ggccgttact
     3241 gttgcgacgg atcattacgc tgggcataca tggactccac tgtcgggaag aagccgggat
     3301 ggaaaatcca tcccgaccgt tttgtcgcta caaacaatcg atccagccgc gatcagcgaa
     3361 tcaggtcg
//
LOCUS       DASUEG010000378         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1239073, whole genome shotgun sequence.
ACCESSION   DASUEG010000378 DASUEG010000000
VERSION     DASUEG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239073"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFX11_06995"
     CDS             complement(<1..674)
                     /locus_tag="VFX11_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938403.1"
                     /translation="MLIPVTAAVVTWVHVWMAMQMVFLPLEFVGYKPLYLGWQGIIPR
                     KTRKMAGIVVDKTLTKLGSLDEVFREMEPERIAEHVSRAVLSRIEDLTDEIMSEKNAV
                     LWENLPNVVKNQVYSRMRRQIPGIMDNLVQAMSANINDLVDIKDMIVRQLESDKGMMV
                     RVFREVGGKEIRFVVNSSFWIGLGFGFVQMVLWYFVPWHMGLPLYAAVLGALTNWLAL
                     TMVFRPL"
     gene            complement(930..1811)
                     /locus_tag="VFX11_07000"
     CDS             complement(930..1811)
                     /locus_tag="VFX11_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4938404.1"
                     /translation="MPISSGWPLQKGAIRFFVPHFVAHHLAQHPLSRELYINGVGFYP
                     QARHHSMTRTEHEDNLLLYCSGGHGEVQAHGQRFAVTKGDLVLLPQGLLHQYAANDQD
                     PWSIHWVHFAGSQAAAFVDHLAFPAGTCVVPVGPHPKLIADFDALLSVRKTGYRLNAF
                     IHASQVLKQSLTYLAMLSTSRVRQTGHFLDLDAIQAVMEENLHGELDLDTLAAIAHLS
                     KYHFCKRYKALTGYSPIQHFIHLKIERACYLLDLSPEPVSAVASSLGYDDAHYFSRLF
                     RKVTGVSPQQYRRLERG"
     gene            1946..>2214
                     /locus_tag="VFX11_07005"
     CDS             1946..>2214
                     /locus_tag="VFX11_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4938405.1"
                     /translation="MTAKPATPVQVPQLIDGEFRQSQSRDWIDVTDPATQAVIARVPC
                     ATDAEMALAIESAKEAFKTWRNTPVPTRARLMLKYQELLKAHHDEL"
BASE COUNT          455 a          641 c          685 g          433 t
ORIGIN      
        1 agcggccgga acaccatagt cagcgccagc cagttggtca gtgcacccag cacggcagcg
       61 tagaggggca ggcccatgtg ccagggcaca aaataccaga gcaccatctg gacaaacccg
      121 aagcccaggc caatccagaa cgatgaattg acgacgaagc ggatttcctt gccacccact
      181 tcccggaata cccgcaccat catgcctttg tcggattcca gctgacggac gatcatgtcc
      241 ttgatgtcga ccagatcatt gatgttggcg ctcatggcct gcaccaggtt gtccatgatg
      301 ccgggaatct gacggcgcat gcgggagtaa acctggttct tcaccacgtt gggcaggttc
      361 tcccagagca cggcattttt ttccgacatg atctcgtcgg tcagatcttc gatgcgcgac
      421 agcaccgctc gcgacacatg ctcggcgatg cgctccggtt ccatctcgcg aaacacttca
      481 tccagggaac ccagtttggt cagggttttg tctaccacga tgccggccat cttgcgggtc
      541 ttgcgcggga tgatgccttg ccagcccaga tacagtggtt tgtaaccaac gaattccagc
      601 ggcaggaaaa ccatctgcat ggccatccac acgtggaccc aggtgacaac ggcggcggtg
      661 acgggaatca gcatgaaacc ccaaaagtca ggccgggcga caagactgtg ccaaaactcc
      721 attatgtcca tgtcgattct gtattttttt tgggtttgtg agtcggtttg tgagagccgg
      781 acggtcgggc tgctgctccg tgtgcagcgg cacgcgcaac caccgttcgg aagtgcgcta
      841 gcatgattta cgcgcattat aagtgcaatg gctgcgcccc cgttcctgca atttttttcg
      901 agccaatggc acgggtgtgg ctggaaaggt cagccccgtt ccagccggcg gtactgctga
      961 ggtgaaacgc cggtgacctt gcggaacaaa cgcgaaaaat aatgggcgtc gtcgtagccc
     1021 agtgaactgg ccacggcgct gaccggttcc ggcgacaggt ccagcaggta gcaggcgcgc
     1081 tcgattttca ggtggatgaa atgctggatg ggcgagtagc cggtgagggc cttatagcgt
     1141 ttgcagaaat ggtatttcga caggtgggca atggcggcca gggtgtccag gtccagttcg
     1201 ccgtgcaggt tttcctccat gacggcctgg atcgcgtcca gatcgagaaa gtgtccggtc
     1261 tggcgtacgc ggctggtgct gagcatggcc agataggtga ggctctgttt cagcacctgg
     1321 ctggcgtgga tgaacgcgtt caggcggtag ccggtcttgc gtacgctgag cagggcgtcg
     1381 aaatcggcga tcagtttcgg gtgtggcccc accgggacca cgcaggtgcc cgccggaaac
     1441 gcaagatgat ccacgaaggc ggcggcctgg ctgccagcga agtgcaccca gtgaatgctc
     1501 caggggtcct gatcattggc agcgtactgg tgcagcagac cttggggcag caggaccaga
     1561 tcgcccttcg tgacggcgaa gcgctggccg tgggcctgta cctcgccatg accgccgctg
     1621 cagtacagca gcaggttatc ctcgtgttcc gtgcgggtca tgctgtggtg cctggcctgg
     1681 gggtaaaagc ccacgccatt gatgtacagc tcccgcgaca gcggatgttg cgccaggtgg
     1741 tgggccacga agtgcggcac gaaaaaacgg atggcgcctt tctgcagcgg ccagccggag
     1801 gagatgggca tggggcgtcc aggtggagtt ggttgttcgc aatatagtcc acctctggcg
     1861 caacttcgtc aatgtcagcc ggttctggac tatgatacaa atgctcatcc aaccggattc
     1921 cgcacccgca gcaagagata tcgccatgac cgccaagccc gccacacccg tccaggttcc
     1981 ccaattgatc gacggcgaat tccgccagag ccagtcccgc gactggatcg atgtgaccga
     2041 tcctgcaacc caggccgtga ttgcccgcgt gccctgcgcc accgacgccg aaatggcgtt
     2101 ggccatcgag tcggcgaaag aagccttcaa gacctggcgc aacacgccgg tgcccacccg
     2161 ggcgcggctg atgctgaagt accaggaatt gctgaaggcg catcacgacg aact
//
LOCUS       DASUEG010000379         4297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_941221, whole genome shotgun sequence.
ACCESSION   DASUEG010000379 DASUEG010000000
VERSION     DASUEG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941221"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFX11_07010"
     CDS             complement(<1..949)
                     /locus_tag="VFX11_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938406.1"
                     /translation="MYEIKNHPVDPAYAKKANITQDQYQRWYKESIEQPEQFWAARAS
                     EFLTWFKPWSKVKEADFKKGQATWFKDAKLNVSYNCIDRHLEKRADQVAIIWEGDEPS
                     RDKKITYDQLHEEVCRLANVLKQRGVKKGDPVCIYMPMIPEAAYAMLACTRIGAVHSV
                     VFGGFSPESLKDRILDSDCRVLITADEGLRGGKKIPLKANADTALSHCPNVHTCLVVR
                     HTGGTINWNEKHDVWYHAVRDKVSDECPAEEMDAEDPLFILYTSGSTGKPKGVLHTTA
                     GYLLGAALSHKYVLDYREGDIFWCTADVGWVTGHSYILYG"
     gene            1602..3758
                     /locus_tag="VFX11_07015"
     CDS             1602..3758
                     /locus_tag="VFX11_07015"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX4938407.1"
                     /translation="MSAVNFKPVAKQEEREIPLQEASYDIWRTKYCLQNSEGEAIDAS
                     IDHSFQRVARALADVETTDELKAKWYDKFLWALRHGAIPAGRILSNAGAQAYKADTST
                     INCTVSGTINDTMSGILEKNYEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAKTSGPL
                     SFMDIFDRTCFTVSSAGGRRGAQMGTFDVSHPDVLEFVKAKREDGRLRQFNLSLLISE
                     EFINAVVKDERWPLVFPIKTNSPDATKLDMKDVTQVIWREWPANDGYVTNDKGLVACK
                     VYKQEKARKIWHAIMSSTYDFAEPGFILIDKVNEMNNNWFCEQIRATNPCGEQPLPPY
                     GSCLLGSVNLTRYVRNPFTDKAYFDWDQYKEVVATFTRLLDNVVEVNGLPLEKQAHEI
                     KHKRRHGMGFLGLGSTLTMLGMKYGSSESVEFTEEVSKQLALTGWKTGLELAKEKGPA
                     PIMDEDFVVDGAMLRKRPEMKKDGYKIGDKVKGKVLHAKYSRYMNRIAQADPELVKQL
                     AEVGSRFTHHSSIAPTGTISLSIANNASNGIEPSFAHHYSRNIIREGRKSKEKVDVFS
                     YELLAYRELVNSDAMPYSTEADKKLPDYFTASDEVTPEQHVDIQAAAQKWVDSSISKT
                     INVPTDFEYEKFKDIYLYAFRKGLKGCTTFRFNPEAFQGVLVKEKDIENTTYRFTLAD
                     GTVVEAKGNEDILYDGEVHSAANLYDALKEGYYGKL"
     gene            3788..>4297
                     /locus_tag="VFX11_07020"
     CDS             3788..>4297
                     /locus_tag="VFX11_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HEX4938408.1"
                     /translation="MAKEIKTKIVGYEVVKPESKNAPAPVVVEPPKSNVVQMHESVER
                     PEMLLGSTYKIKTPLSDHALYITINDIILNEGTEHEQRRPFEIFINSKNMENFQWVLA
                     LTRVISAVFRKGGDATFLVEEMKAVFDPKGGYFKKGGKFMPSLVAEIGEAIETHMKMI
                     GMIKGEMDPH"
BASE COUNT         1014 a         1265 c         1196 g          822 t
ORIGIN      
        1 gcccgtaaag aatgtaggaa tgaccagtga cccagcccac gtcggcggta caccagaaaa
       61 tgtcgccctc gcggtaatcc agcacgtatt tgtggctcag ggccgccccc agcagatagc
      121 cggcagtggt gtgcagcaca cccttgggct tgccggtgga gccggaggtg tacaggatga
      181 acagcggatc ttccgcgtcc atttcctcgg ccgggcactc gtcgctcacc ttgtcgcgca
      241 ccgcgtggta ccacacgtcg tgcttctcgt tccagttgat cgtgccgccg gtgtgacgca
      301 ccaccaggca ggtgtgcacg ttggggcagt ggctcagcgc cgtgtcggca ttggccttca
      361 gggggatttt cttgccaccg cgcaggccct cgtccgccgt gatcagcacc cggcaatcgg
      421 aatccaggat gcgatccttc agcgactccg gcgaaaagcc accgaacacc accgaatgca
      481 cggcgccgat gcgggtgcag gccagcatgg cgtaggccgc ttccggaatc atcggcatat
      541 agatgcacac cgggtcgcct ttcttcacgc cacgctgctt gaggacattg gccagccggc
      601 acacttcctc gtgcagctga tcgtaggtga tcttcttgtc ccggcttggt tcatcacctt
      661 cccagatgat cgctacctga tcggcgcgct tttccagatg acggtcgatg cagttgtagc
      721 tgacattcag cttcgcatcc ttgaaccagg tggcctgacc ctttttgaaa tccgcttcct
      781 ttaccttgct ccagggcttg aaccaggtca gaaactcgga ggcgcgggcg gcccagaact
      841 gctccggctg ctcgatggat tccttgtacc agcgctggta ctggtcctgc gtgatgttgg
      901 cctttttcgc gtaggcgggg tcgaccggat ggtttttgat ttcgtacatg aaacgcgtgc
      961 tcctgcagtg gtacttcaat ctggaacaaa gaaaaacaag ttaacccccg agggggaaaa
     1021 tcgcaatccg gccggccttg aagcgcgctt catgacattt ttttgttgta tagaaaacaa
     1081 tcagataagg agctgctaac agcctgcccc ttgccagcgt tggggggtaa gccccggtat
     1141 acaaaatggt gagttacaaa ctgtaaaaaa actcaaaaat tggggttgtg aaatttgcga
     1201 cgctgactag aatgattttc gaaaccacaa gatattgtgg ttggcgacat gagaaaggcg
     1261 gatatagcgt gtttcgctgt tttcgggtgg cggagggatt ccgaacccgt gaaagctgtg
     1321 cgagcgcgcc ggaccccact gtgcagggct cgattttgca tggctcatac aacaaaacga
     1381 actgtcgtcg aaggggtgga atcatgcaag gcacgtcctg cttcagccaa ccagcaaacg
     1441 caacaccatc cgcagtttct tacctggatt cgattttcgg gccgctcgcg cgcccgtttt
     1501 gatcacttta gcgcatcgat acacacgctt tgggtacata gggaaccggc cccccggggg
     1561 cgcggacatc aatttcattc ggaaacaaga agaaggtggc aatgagcgct gtcaatttca
     1621 aaccagtagc gaagcaagaa gaacgggaaa ttcccctgca agaagcgtct tacgacatct
     1681 ggcgtaccaa gtactgcctg cagaacagcg aaggggaagc catcgatgcc agcatcgacc
     1741 actccttcca gcgcgtggcc cgcgccctgg ccgacgtgga aaccaccgac gaactgaaag
     1801 ccaagtggta cgacaagttc ctgtgggccc tgcgccacgg cgccattccc gccggccgca
     1861 tcctgtccaa cgccggtgcc caggcctata aagccgacac ctccaccatc aactgcaccg
     1921 tgtccggcac catcaacgac accatgtccg gcattctgga gaagaactac gaggcaggcc
     1981 tcacgctcaa ggccggctgc ggtatcggtt acgagttctc caccctgcgt ccccgtggcg
     2041 cctacgtgtc cggtgctggt gctaaaacct cgggcccgct gtcgttcatg gacatcttcg
     2101 accgcacctg cttcaccgtg tcctctgccg gtggccgccg tggcgcgcag atgggcacct
     2161 tcgacgtgtc ccacccggat gtactggaat tcgtcaaagc caagcgtgaa gacggccgtc
     2221 tgcgccagtt caacctgtcg ctgctgatca gcgaggaatt catcaacgcc gtggtgaaag
     2281 acgaacgctg gccgctggtg ttcccgatca agaccaacag ccccgacgcc accaagctgg
     2341 acatgaaaga cgtgacccag gtgatctggc gcgaatggcc cgccaacgac ggctacgtca
     2401 ccaacgacaa aggtctggtg gcctgcaaag tctacaagca ggaaaaagcc cgcaagatct
     2461 ggcacgccat catgtcgtcc acctacgatt tcgccgaacc cggtttcatc ctgatcgaca
     2521 aggtcaacga gatgaacaac aactggttct gcgaacagat ccgagccacc aacccctgcg
     2581 gcgaacagcc cctgccgccc tatggcagtt gtctgctggg ttccgtgaac ctgacccgct
     2641 acgtgcgcaa cccgttcacc gacaaagcct acttcgactg ggatcaatac aaggaagtgg
     2701 tcgccacttt cacccgtctg ctcgacaacg tggtggaagt gaacggcctg ccgctggaaa
     2761 aacaggccca cgagatcaag cacaaacgcc gtcacggcat gggctttctg ggcctgggtt
     2821 ccaccctcac catgctgggc atgaaatacg gctcgtctga atccgtggag ttcaccgagg
     2881 aagtgtccaa gcagctggcg ctgaccggct ggaaaaccgg cctggaactg gcgaaggaaa
     2941 aaggcccggc gccgatcatg gacgaagatt tcgtggtgga tggcgccatg ctgcgcaagc
     3001 gccccgaaat gaaaaaggac ggctacaaga tcggcgacaa ggtaaaaggc aaagtgctgc
     3061 acgccaaata tagccgctac atgaaccgca tcgcccaggc cgacccggaa ctggtgaagc
     3121 aactggcgga agtgggttcg cgctttaccc accattcgtc catcgcaccc accggcacca
     3181 tttcgctgtc gattgcgaac aacgccagca acggcatcga gcccagcttt gcgcatcact
     3241 actcgcgcaa catcattcgt gaaggccgca agtcgaaaga gaaagtggat gtgttctcgt
     3301 acgaattgct ggcctaccgc gagctggtca acagcgacgc catgccgtac agcaccgaag
     3361 ccgacaagaa actgccggac tacttcaccg ccagcgatga agtcaccccc gagcagcacg
     3421 tggacatcca ggccgccgcg cagaaatggg tggattcctc gatttccaaa accatcaacg
     3481 tgcccaccga cttcgaatat gagaagttca aggacatcta cctgtacgct ttccgcaagg
     3541 ggctgaaggg ttgcaccacc ttccgtttca atccggaagc gttccagggc gtgctggtga
     3601 aagagaaaga catcgaaaac accacctacc gcttcaccct ggccgacggc accgtggtgg
     3661 aagccaaggg taacgaagac atcctgtatg atggcgaagt gcactccgcc gctaacctgt
     3721 acgacgcctt gaaagaaggc tattacggta aactctaatg gacgacaaag attcgggaag
     3781 caagcccatg gctaaagaaa tcaaaaccaa aatcgttggc tacgaagtgg tgaaaccgga
     3841 gtcgaaaaat gcaccggcac ccgtggtagt ggaaccgccg aaaagcaatg tggtgcagat
     3901 gcacgaaagc gtcgagcgcc cggaaatgct gctgggttcc acctacaaga tcaaaacccc
     3961 gctgtcggac catgcgttgt acatcaccat caacgacatc attctgaatg aaggcactga
     4021 acacgagcag cgtcgcccct tcgagatctt catcaactcg aagaacatgg agaacttcca
     4081 gtgggttctg gcgctgacgc gcgttatttc ggccgtgttc cgcaagggcg gtgacgctac
     4141 cttcctggtg gaagagatga aggccgtgtt tgatccgaaa ggcggttact tcaagaaggg
     4201 cggcaaattc atgccgtcgc tggtggcgga gatcggtgaa gcgattgaaa cccacatgaa
     4261 gatgatcggc atgatcaagg gcgagatgga cccgcac
//
LOCUS       DASUEG010000380         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_382082, whole genome shotgun sequence.
ACCESSION   DASUEG010000380 DASUEG010000000
VERSION     DASUEG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382082"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..479
                     /locus_tag="VFX11_07025"
     CDS             141..479
                     /locus_tag="VFX11_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4938409.1"
                     /translation="MRMITAIIKPFKLDDVREALTDVGINGMTVEEVKGFGRQKGHTE
                     LYRGAEYVIDFVPKLKLQLAVRTDQVDDAISAITRAANTGKIGDGKIFVTDLEQVIRV
                     RTGEQGTDAL"
     gene            525..1850
                     /locus_tag="VFX11_07030"
     CDS             525..1850
                     /locus_tag="VFX11_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4938410.1"
                     /translation="MLSKTIRRLSQGILLSCVPVISHAEELNGAAINPANTAWMLTAT
                     ALVLFMTIPGLSLFYAGLVRSKNVLSVLMHCFALTCAMSILWFVVGYTMAFGSAPEQG
                     DFLGDFGNVLFAAIKLDGVTLGIPTALFAIFQMTFAIITPALIVGAFAERMRFSAMLM
                     FSVLWLLLVYAPVCHWVWGGGWLQDMGLLDFAGGTVVHITAGVAALVAALVLGPRKGF
                     PTQAMMPHNLTMTVTGAGMLWVGWFGFNAGSAVAANGNAAMAMLVTHLSAAAGSMAWM
                     ACEWIRHGKPSVLGIVTGMVAGLGTITPASGSVGPAAALVIGLCAGTICFFMTMLIKK
                     RLHIDDSLDVFPVHGVGGILGTLLAGIFCSPDLGLFSGLGFGAGNQSISDQLGVQAIG
                     VVSTFLYTAVVTWILLKLVGMLVDLRVTGEEETEGLDIVQHDERGYDIR"
     gene            2075..2467
                     /locus_tag="VFX11_07035"
     CDS             2075..2467
                     /locus_tag="VFX11_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX4938411.1"
                     /translation="MSLKFLSDEWFSKVEELKAAAGAVEVPPAMTDLVINITVSGTEW
                     GNRDMSLKAGSIEKGHHAGAPTKMTLPADLARRLFIENDQSAGMQGFMSGQIKIEGDM
                     SKLMAMQTVQPSASQKALQKQILEATAK"
     gene            2719..3567
                     /locus_tag="VFX11_07040"
     CDS             2719..3567
                     /locus_tag="VFX11_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX4938412.1"
                     /translation="MAEPISSALIGLTSEPATFSWSEKDVQLYALAVGCKPDEELDFI
                     YEARGPKVLPTFSVIPGLTIMGSVVGRVQFNLAMLLHGEQKLTLHRPIPASGTATGVG
                     KIVEVWDKGKAAVIGVQCEVSDAQGPLFTTHATLFIRGAGGFGGERGPASEATAIAIP
                     DRAADHVVEDVTRPEQAALYRLTGDRNPIHIDPMFAKFGGFERPFLHGLCTYGFAGRA
                     ALKALCGGDPARFISMSGRFSDQVYMGDSIITRIWEEGNGVAVMQVETQKGNVVLNQC
                     RVEYRG"
BASE COUNT          725 a          973 c         1139 g          808 t
ORIGIN      
        1 tttcagatct taaaattcta agctattgaa aaatatagaa tttaaaggct ggcccggatt
       61 ctgcatgctc tcgtttggtg cgggagcacc aaattggttc aaaaataaaa cccgaacaac
      121 aaaatcaagg gcgcgcaatt atgagaatga tcacggcaat catcaagcct tttaagctcg
      181 atgatgtcag ggaagccttg accgatgtgg ggataaacgg catgacggtt gaggaagtga
      241 aagggttcgg ccgccagaag ggccatacgg aactgtaccg gggggcggaa tatgtgatcg
      301 atttcgtgcc caagctgaaa ctgcagctgg cggtgcggac cgatcaggtc gatgacgcca
      361 tcagcgcgat tacccgtgcg gccaacacgg gcaaaatcgg cgatggcaag attttcgtga
      421 ctgatctgga acaggtcatt cgcgtccgga ccggtgaaca gggcaccgac gcactctgat
      481 ccaataccca tttccaatgc actaaatcct tcaaggggat ctccatgctg agcaaaacca
      541 tacgacgtct gtcgcagggc attctgttgt cctgcgttcc cgtaatttcc cacgcggaag
      601 aactcaatgg cgccgccatc aatcctgcaa acaccgcctg gatgctgacg gccaccgccc
      661 tggtgctgtt catgaccatt cccggcctgt cgctgtttta cgcggggctg gtgcggtcga
      721 aaaacgtgtt gtcggtgctg atgcactgct ttgcgctgac ctgtgccatg tccattctct
      781 ggtttgtcgt gggctatacc atggcgtttg gatcggcacc ggaacagggt gactttctgg
      841 gcgatttcgg caatgtgctg tttgcggcga tcaagctgga cggtgtgacc ctgggtattc
      901 ccactgcact gttcgccatt ttccagatga cgtttgccat catcacgccg gccctgatcg
      961 tgggtgcgtt tgccgagcgg atgcggtttt ccgccatgct gatgttctcg gtcctgtggc
     1021 tgctgctggt gtatgcaccg gtgtgccact gggtatgggg cggtggctgg ctgcaggaca
     1081 tgggtctgct ggactttgcc ggtggcacag tggtgcacat cactgccggt gtggcggcgc
     1141 tggtggcggc gctggtactg gggccgcgca agggctttcc cacccaggcc atgatgcccc
     1201 acaacctgac catgaccgtg accggcgccg gcatgctgtg ggtgggctgg tttggcttca
     1261 acgccggcag cgcggtggcg gccaatggca atgcggcgat ggcgatgctg gtgacccacc
     1321 tgtcggcggc ggccggctcg atggcctgga tggcctgtga atggatacgc catggcaagc
     1381 cgtcggtgct gggtattgtc accggcatgg tggcgggcct gggcaccata accccggcgt
     1441 caggctcggt ggggccggcg gcggcgctgg tgatcggcct ttgcgccggc actatctgct
     1501 tcttcatgac catgctgatc aagaaaaggc tgcatatcga cgattcgctg gatgtgttcc
     1561 cggtgcacgg tgtgggcggg attctgggta ccctgctggc gggcatattc tgttcaccgg
     1621 atctgggcct gttcagcggt ctgggttttg gcgccggcaa ccagagcatc agtgaccagt
     1681 tgggcgtgca ggccatcgga gtggtttcca cattcctcta taccgcggtg gtcacctgga
     1741 ttctgctgaa gctggtgggc atgctggtgg atctgcgggt caccggcgaa gaggaaaccg
     1801 aaggtctgga tatcgtgcag cacgacgagc gtggctacga tattcgctga tgatggtttt
     1861 tgcggcgtta ccggccggta gcgccgcatt tccctaattg accaaaaaag cccgttcctt
     1921 ggttaaattg gctattgaac aacgcggggg gatttaatac tttaccggca agcgtctcga
     1981 acagtcgttt gattggcaag gcgtccctat cgactccgat gccggagtga ccactcagaa
     2041 aacgagtgac tataaaaatt acgaggagat gaagatgagc ttgaaattcc tgtctgacga
     2101 atggttttcc aaagtagaag aactgaaagc ggctgctggc gcagttgaag ttccccccgc
     2161 catgactgat ctggttatca acatcaccgt gagcggcacc gagtggggca accgcgacat
     2221 gagcctgaag gccggttcca tcgagaaagg tcatcacgct ggcgcgccca ccaaaatgac
     2281 tctgcccgct gatctggccc gtcgtctgtt catcgagaac gaccagtctg ccggtatgca
     2341 gggtttcatg agcggccaga tcaagatcga aggcgacatg agcaagctga tggccatgca
     2401 gaccgtgcag ccttccgctt cccagaaagc cctgcagaag cagatcctgg aagccactgc
     2461 aaagtgattt cagcgttccg ctgatgccga gaagcccgcc tagtgcgggt ttttttgtgc
     2521 ctgccgttcg ggctctggtg caaattgtca acgaaaatga cttcattggt cattggcgcg
     2581 gcgacagcgg ggtggttaat tggcgggagt gcgcgtttga tagacaaatc agaatgcaga
     2641 cgcgcatttt ctgacacagg gagccgttca tcccgctacc cgcatttatc cccaacaata
     2701 tgacaagagg gaaccaccat ggcagaaccg atttccagcg ccctgatcgg attgacgtcc
     2761 gaacccgcca cgttttcctg gtccgagaag gatgtgcagc tgtatgcgct tgccgttggc
     2821 tgcaagccgg atgaggaact ggatttcatc tatgaagccc gcgggcccaa ggtgctgccg
     2881 accttcagtg tgattcccgg cctgactatc atgggcagcg tggtgggacg tgtgcagttc
     2941 aatctggcga tgctgctgca cggcgagcag aagctcacac tgcaccggcc gattcctgcc
     3001 agcggtaccg ctactggcgt gggtaaaatc gtggaagtgt gggacaaggg caaggccgcc
     3061 gtgatcggtg tgcaatgcga ggtgagtgat gcgcagggcc ctctgttcac cacccatgcc
     3121 actctgttca ttcgcggtgc cggcggtttt ggcggtgaac gtggtccggc gtcggaagcc
     3181 actgcgattg caataccgga tcgtgcggct gatcatgtgg tggaagatgt gacgcggccg
     3241 gagcaggcgg cgctgtaccg cctcaccggc gaccgcaatc cgattcacat cgatccgatg
     3301 ttcgccaaat tcggcggttt cgagcggccg ttcctgcacg gtctgtgcac ctacggtttt
     3361 gccggccgcg cggcgctgaa agcactgtgc ggcggcgatc cggcgcgctt catttccatg
     3421 agcggccgtt tttccgacca ggtgtacatg ggggattcga tcatcacgcg catctgggaa
     3481 gagggcaatg gtgttgccgt catgcaggtg gaaacacaaa aaggcaatgt ggtgctgaat
     3541 cagtgccggg ttgaataccg gggctgagca gcgctcagta cccggccttg cctgccatga
     3601 tcagttcgta gaatgcccgg tccagcggct cgtaaaagct gctgc
//
LOCUS       DASUEG010000381         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1413426, whole genome shotgun sequence.
ACCESSION   DASUEG010000381 DASUEG010000000
VERSION     DASUEG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413426"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFX11_07045"
     CDS             complement(<1..1139)
                     /locus_tag="VFX11_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938413.1"
                     /translation="MELPFSSTPQTPPPRRPQMAVSFAAPAAAGGGGLMDTVSSVAAA
                     VGIDLSSDAVDPWQRSVTTIVCDSHLAPRVDQARILLAYDSQSPTFNLGDSGSIELGY
                     AEDQLYSVFSGTIIGIQSALERQAALTIGNGGQRLSQLRTNQSFEQQSAGDIVQALAD
                     LAEVETGTVEAGADLPFYVVDDGRNLYQHIATLAEKSGLLACIDGDGKLQFREAPGSA
                     PEQTFHYGVDILQLRLQRSEPPLQQLTLIGAGAAGSQGSDAWSWLVKDPQSISATVGE
                     GASVRLQADPSLRSSDAVQQAAAGKLFFARQRALKVRLVVLGAPNIRAGSKIEIADAP
                     QPEWNGTAVVESVRHQYSKLKGFTSDITALLESADNSMDSLLAGAL"
     gene            complement(1148..>3254)
                     /locus_tag="VFX11_07050"
     CDS             complement(1148..>3254)
                     /locus_tag="VFX11_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938414.1"
                     /translation="QLNINAIRDFVTRFLQIMLSQFGFSLDTLVERTWGIFDAVMTDL
                     RTLPAGTDAQEKELRLVTASLLGRVKRELQEQVELPAISADDIAAVIMEQFRELGLDT
                     WVQQANQIAEAIKSVCLAAGSIGDLLKVGGSVGAAAKTPDTGDTYAWYASWLLATRNR
                     DIGETIGNFFILNPSDEVWVTADKNQVVWRTVFDGDLVLHEGSNLKWNDAPMFNSATG
                     AEYVLFPHISADAMEFLAHFTYALVELGKGIWNSVDAAEAGDRASAITHLIWNFFNSG
                     FGALGEKPFISYLLAASALGQGHQGWMNAIFPIVGTTLPSLEGRQTEADEAKFAFWGI
                     LFADDVLERAGHHLLLNTVRDLLLSTLTLINNIGQAQGTDASHPINKQYTSTWATVGA
                     MICTIIYLKKWYNKADYKYPFNGFLWTFASMLAGLVGGLSGTLTASLWSIGFDVKSFF
                     VDIAMETIKPFFLHYVNLYSWIENDTKGGTFNGSRPAFAGYPEKSDSPYRLPWETGVT
                     RACVQGNMGMWSHFDASDQIYAVDFGFDQAEVIHAARSGTVVGFRDTVPNDTHDQGWN
                     YIVIRHDHASETNTVRQKHDKYHDGTAYVTYGVYGHGRTNGVTEVFAARGIAAASILG
                     TVVAQGDPIMKAGNTGESFHNHLHFHVKNNGEALGTAAPAGNLNPSTLSSNSMPFVFR
                     DVDGDGVVKSLTFYTASNV"
BASE COUNT          590 a          978 c         1018 g          668 t
ORIGIN      
        1 agcgcgccag ccagcaggct gtccatgctg ttgtcggcgc tttccagcaa ggcggtgatg
       61 tcgctggtga agcctttcag cttgctgtac tggtgacgca cggattcgac gacagcagtg
      121 ccgttccatt ccggctgcgg cgcgtcggcg atttcgattt tgctgccggc gcggatgttg
      181 ggcgcaccca gtacaaccaa gcgtactttc agcgcgcgct gtctggcgaa aaacagtttg
      241 cctgccgccg cctgttgcac cgcgtcgctg ctgcgcaggc tgggatcggc ctgcaggcgg
      301 acgctggcgc cttcgcccac cgttgcgctg atggactgcg gatctttcac cagccagctc
      361 caggcgtcgg agccctggct gccagcggca ccggcgccga tcagggtgag ttgctgcagt
      421 ggcggttcgc tgcgctgcaa acgcagttgc aaaatgtcga caccgtagtg gaaggtttgc
      481 tccggcgcgg aaccgggcgc ttccctgaac tgcaatttgc catcgccatc gatgcaggcg
      541 agcaggccgc ttttttccgc cagcgtggca atgtgctggt acaggttgcg gccgtcgtcc
      601 accacataaa acggcagatc cgcgccggct tccacggtgc cggtttctac ttccgccaga
      661 tccgccagcg cctgcacgat atcgccggcg ctctgctgtt cgaaactctg gttggtgcgc
      721 agttgcgaca ggcgctggcc gccgttgccg atggtgagtg cggcctgccg ttccagcgcg
      781 gactggatgc caatgatggt gccgctgaac acggaataaa gctgatcttc cgcgtaaccc
      841 agttcgatgc tgccactgtc gcccagattg aacgtggggg actggctgtc gtaggccagc
      901 agaatgcgcg cctgatccac gcgcggtgcc aggtggctgt cgcagacgat ggtggtgacg
      961 ctgcgctgcc agggatcgac cgcatcgctg gacaggtcga tgcccaccgc cgccgcgacg
     1021 ctggacacgg tgtccatcaa tccgccacca ccggccgctg ccggcgcggc aaaactcacg
     1081 gccatttgcg ggcgacgcgg gggtggtgtt tgcggtgtgc tggaaaaagg cagttccata
     1141 aaattcgtca gacgttgctc gccgtataaa aggtcaggga tttcaccacg ccgtcgccgt
     1201 ccacatcgcg aaacacgaag ggcatggaat tactgctgag tgtgctggga ttaagattgc
     1261 ccgccggcgc cgcggtgccc agtgcctcgc cattgttctt cacatggaag tgcagatggt
     1321 tgtgaaaact ttcgccggtg ttgcctgctt tcatgatggg atcgccttgt gccaccacgg
     1381 tacccaggat gctggcggcg gcaatgcccc gtgccgcaaa tacttcggtg acaccgttgg
     1441 tgcggccgtg gccgtaaacg ccgtacgtca catacgcagt gccgtcgtgg tacttgtcat
     1501 gcttctgccg caccgtattg gtttcgctgg cgtgatcgtg gcggatgaca atgtaattcc
     1561 agccctggtc gtgggtgtcg ttgggcaccg tgtcgcgaaa acccaccacg gtgccgctgc
     1621 gggcggcgtg gatgacttcc gcctggtcga aaccaaaatc cactgcgtaa atctggtccg
     1681 acgcatcgaa gtggctccac atgcccatgt tgccctgcac gcaggcgcgg gtgacgccgg
     1741 tttcccacgg cagccggtag ggcgaatcgg atttttccgg atagccggca aacgcggggc
     1801 ggctgccgtt gaaggtgccg cccttggtgt cgttctcgat ccagctgtac aggttcacgt
     1861 aatgcaggaa aaacggtttg atggtttcca tcgcgatgtc gacgaagaaa cttttcacat
     1921 cgaagccgat ggaccacagg ctggcggtga gggtgccgga caatccgccg accaatcctg
     1981 ccagcatgct ggcgaacgtc cacagaaaac cgttgaacgg atatttgtag tcggccttgt
     2041 tgtaccactt cttcagatag atgatggtgc agatcatggc gccgacggtg gcccaggtgc
     2101 tggtgtattg cttgttgatg ggatggctgg cgtcggtgcc ctgggcctgg ccgatgttgt
     2161 tgatcagcgt cagggtggac agcagcagat cgcgcacggt gttcagcagc agatggtgtc
     2221 cggcccgttc cagcacatcg tcggcaaata aaatgcccca gaacgcaaac ttggcttcgt
     2281 ctgcttcggt ctggcggcct tccagcgatg gcagcgtggt acccacgatg ggaaaaatcg
     2341 cattcatcca gccctgatgg ccctggccca gcgccgatgc cgccagcaga taggaaatga
     2401 acggcttctc acccagtgcg ccaaaaccgc tgttgaagaa attccagatc agatgggtga
     2461 tggcactggc gcggtcaccg gcttccgctg catcgacgga gttccagatg cctttgccca
     2521 gctccaccag cgcgtaggta aagtgcgcca ggaattccat cgcatccgca gagatgtgcg
     2581 ggaacaacac gtactcggcg cccgtggcgg aattgaacat gggcgcgtcg ttccacttca
     2641 ggttgctgcc ctcgtgcagc accagatcgc cgtcaaacac cgtgcgccat accacctggt
     2701 ttttgtcggc ggtgacccag acttcgtccg acggattcag aataaaaaag ttgccaatgg
     2761 tttcgccgat gtcgcggttg cgggtggcca gcagccagct ggcgtaccag gcgtaggtgt
     2821 cgccggtgtc cggtgttttt gctgcggcgc cgacactgcc gcccaccttc agcagatcgc
     2881 cgatggaacc ggctgccaga cacacggact tgatcgcctc ggcaatctgg ttggcctgct
     2941 gcacccaggt gtccagaccc agttcccgga actgctccat gatcacggca gcgatgtcgt
     3001 cggcggatat cgccggcagt tccacctgct cctgcagttc ccgtttcacc cggcccagca
     3061 ggctggcggt gaccagacgc agctcctttt cctgggcgtc ggtgccggcg ggcagggtac
     3121 gcagatccgt catcaccgca tcgaaaatgc cccaggtgcg ctccaccagc gtatccagcg
     3181 aaaagccgaa ctggctgagc atgatctgca gaaagcgggt gacgaaatcg cggatcgcat
     3241 tgatgttcag ctgt
//
LOCUS       DASUEG010000382         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2011449, whole genome shotgun sequence.
ACCESSION   DASUEG010000382 DASUEG010000000
VERSION     DASUEG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011449"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1869)
                     /locus_tag="VFX11_07055"
     CDS             complement(175..1869)
                     /locus_tag="VFX11_07055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4938415.1"
                     /translation="MSSTMSSTTTSAIEQARSLIQQAQQSLNAALSFLKQESLVNGRV
                     NGDKLDRKQLACYEVAYCEAEITAAKVMLDYSQKVKAEKGGSDLRIEERLALQYTAEA
                     LQNCRGRFNARRADFGFSREQLRASLDSDAVDAFINQYLSTESLEAVGKAVLHNDGFT
                     GEYMLDHEKEMMRDQFRSFASNVVAPLAEKIHTHDLDIPDAIIKPLVELGCFGLSIPM
                     QYGGIQPDDSEDNLGMIVVTEELSRGSLGAAGSLITRPEIMARALLKGGTEEQKQHWL
                     PKLAQAEPLNAVAVTEPNFGSDVASMKLKATQVDGGWILDGEKTWCTFAGRAGVLLTL
                     ARTNADPASGHRGLTMFLVEKPSFPGHAFEYKQPQGGTITGKAIPTIGYRGMHSFTVF
                     FDHYFVPDSHVLGGKEGIGNGFYYTMAGFSGGRIQTAARATGVMQAAFEKALSYSKER
                     QVFGKPIGDYQLTLVKIARMAMLLAVSRQFTYHVARLMDEGKGQMEASLVKLYSCKSS
                     EWLAREAMQIHGGMGYAEETSVSRYYIDARVLSIFEGAEETLALKVIAKALVENAA"
     gene            complement(1942..3372)
                     /locus_tag="VFX11_07060"
     CDS             complement(1942..3372)
                     /locus_tag="VFX11_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEX4938416.1"
                     /translation="MKLTLTDADTQSILSRLADSNRKHSSVYPGDSSARQQVHTVYGG
                     ANLFKAGGAQKLGSLALKALETYAPDFCTLARVLGFPGAESLPSTSFEIKTLAAAIEA
                     DPAGLKNSNPAAWTAHTVYARVVKKLQSEPVEDSRIDFEDGYGNRPDAEEDADAVRCA
                     EEVAKGMEEKNLPPFIGIRIKTFTEECRKRSVRTLDLFLTRLAELTKGALPDNFVITL
                     PKVTHPEQVTALADLLDIIEAKCGYAKNSLKLEIMIETTQAIIDSKGSNQVLLLAEAA
                     RGRCRSAIFGTYDYTATCNITAAFQTHTHPASDFARHVLQVSLAGTGITISDGATTVM
                     PIGPHKGEMTTQQILENAAVVHSAWKLHYDNIRHSLRHAYYQGWDLNPGQLPIRYAAI
                     YAFFMEGLPDASRRLGAFINKAAQATLVGNTFDDAATGQGLLNFFLRGIACGAITEQE
                     ALATGITMDELRSRSFVHIVSNRAHG"
BASE COUNT          733 a         1113 c         1010 g          755 t
ORIGIN      
        1 taacgaagcg agaaccggtc gctagcacga gccctcagga gcggcgcaga gtttcaggaa
       61 cgtcagcggc catggatggc cgctgacgag cctacaggga tgtacttgtg gcgtttcctg
      121 aaactctgcg tcgctcctga gggcatccac aatagaatca cgaacactta agcgttacgc
      181 cgcattctcc accagcgcct tcgcaatcac cttcagcgcc agcgtctctt ccgcaccttc
      241 gaaaatcgac agcacccgcg catcgatgta ataccggctc acggacgtct cttccgcata
      301 acccatacca ccgtgaatct gcatcgcttc ccgcgccagc cactccgacg acttgcagga
      361 atacagcttc accagactcg cctccatctg gcccttgcct tcatccatca aacgcgccac
      421 gtgataagtg aactgccgcg acaccgccag caacatcgcc atacgggcaa tcttcaccag
      481 cgtcagctga taatcaccaa tcggcttacc aaatacctga cgctctttcg aataactcaa
      541 cgccttctca aacgccgcct gcatcacacc ggtcgcgcgt gcagccgtct gaatccggcc
      601 gccggaaaag cctgccatcg tgtaatagaa gccgttgcca atcccttcct tgccacccag
      661 cacatgggaa tccggcacaa aatagtgatc gaagaacacc gtgaatgaat gcatgccgcg
      721 atacccgatc gtcggaatcg ccttgccggt aatcgtgcca ccctggggct gcttgtactc
      781 aaacgcatga cccgggaacg aaggcttctc caccagaaac atcgtcaacc cgcgatgccc
      841 cgacgcagga tcggcattgg tacgcgccag cgtcagcagc acaccggcac ggcctgcaaa
      901 cgtacaccag gttttctcgc catccagaat ccagccgcca tccacctgcg tggccttcag
      961 cttcatcgat gccacatccg atccaaaatt cggctcggtc acggccaccg cattcagcgg
     1021 ctccgcctgc gccagcttcg gcaaccagtg ctgcttctgc tcctcggtac cacctttcag
     1081 cagcgcccgc gccataatct ccggacgggt aatcaaactg cctgccgcac ccagcgaacc
     1141 gcgcgacaat tcctccgtca ccacaatcat gcccagattg tcttcgctgt catccggctg
     1201 aatcccgccg tactgcatcg gaatcgacag accgaagcaa cccagctcca ccagcggctt
     1261 gatgatcgcg tcaggaatat ccaggtcgtg ggtatgaatc ttctccgcca gcggagccac
     1321 cacattgctg gcaaacgagc ggaactggtc ccgcatcatt tccttttcgt ggtccagcat
     1381 gtattcgccg gtgaaaccgt cgttgtgcag cacggccttg cccaccgctt ccagcgattc
     1441 agtggacagg tactggttga taaacgcatc caccgcatcg gaatccagcg acgcgcgcag
     1501 ctgctcgcgg gaaaaaccga aatccgcccg gcgtgcattg aaacgccccc gacagttctg
     1561 cagcgcttcc gccgtatatt gcagcgccag ccgttcctcg atgcgcagat cgctgccacc
     1621 tttttccgct ttcactttct ggctgtaatc cagcatcact ttcgcggcgg tgatttccgc
     1681 ttcgcaatag gccacttcat agcaggccag ctgcttgcga tccagcttgt cgccgttgac
     1741 acggccgttc accaggcttt cctgtttcag aaagctcagg gcggcattca gcgattgctg
     1801 ggcctgctgg atcagggaac gggcttgttc gattgctgag gtcgttgttg acgacattgt
     1861 tgacgacatg gggggtctcc cttggtcgaa cggctgaaca atcgcaaaat cattcagccg
     1921 ccgagaattg aaaatgaaaa atcagccgtg cgcgcgattc gacacaatgt gcacgaacga
     1981 acgggaccgc agttcatcca tggtgatacc ggtggccagc gcttcctgtt cggtgatggc
     2041 gccacaggcg ataccccgca ggaagaagtt cagcaggccc tggcccgtgg ccgcgtcgtc
     2101 aaaggtattg cccaccagcg tggcttgcgc ggccttgttg atgaacgcac ccaggcggcg
     2161 cgacgcatcc ggcagacctt ccatgaagaa tgcataaatc gcggcataac ggatcggcag
     2221 ctggcccggg ttcaaatccc agccctggta ataggcatgg cgcagcgagt ggcggatatt
     2281 gtcgtagtgc agtttccagg cagaatgcac cacagcggcg ttttccagaa tctgctgcgt
     2341 ggtcatctcg cccttgtgcg ggccgattgg catcacggta gtggcgccgt cgctgatggt
     2401 gatgccggtg ccggccagcg acacctgcaa tacatggcgc gcgaaatcgg aagccgggtg
     2461 ggtgtgcgtc tggaacgcag cggtgatgtt gcaggtggcg gtgtagtcgt aggtaccgaa
     2521 aatcgccgag cggcaacggc cacgggccgc ttccgccagc agcaacacct ggttggagcc
     2581 cttgctgtcg atgatcgcct gggtggtctc gatcatgatt tccagtttca gcgaattttt
     2641 cgcgtagccg cacttggctt cgataatgtc cagcaggtct gccagtgccg tcacctgttc
     2701 cgggtgggtc actttcggca gtgtgatcac gaagttgtcc ggcagcgcgc ctttggtcag
     2761 ttccgccaga cgggtcagga acaggtccag ggtgcgcacg gaacgcttgc ggcattcctc
     2821 ggtgaaggtc ttgatacgaa tgccgatgaa gggcggcagg tttttctcct ccataccttt
     2881 ggcgacttct tccgcgcaac gcacagcgtc ggcatcttct tccgcatccg ggcggttacc
     2941 gtagccgtct tcaaaatcga tgcggctgtc ttccaccggc tcgctctgca gctttttcac
     3001 cacgcgcgcg tacacggtgt gggcggtcca ggcggcgggg ttcgaatttt tcaggccagc
     3061 ggggtcggct tcgatggcgg cggccagcgt tttgatttca aaggacgtgg agggcaggga
     3121 ttccgcaccg ggaaagccca gcacgcgggc cagggtgcaa aaatccgggg cgtaggtttc
     3181 cagtgctttc agggccaggc tgccgagttt ttgcgcaccg ccggctttga acaggttggc
     3241 gccgccataa acagtgtgta cctgctgacg ggccgaggaa tcgcccgggt aaacgctgct
     3301 gtgtttgcgg ttggaatccg ccagccggga caggatggat tgggtgtcgg catcggtcag
     3361 tgtcagcttc atggtgtgcc caggtcctta aatactatcg gatgaaaagt ggcaaacggc
     3421 atcgaaatgc cgcctgattc aaagggatgg cgctgcgaaa acagtggttg cgagaaggct
     3481 gactatttta gcggcgccct tgattgatga atagattata gtgtggacgg ccagtcacag
     3541 gttcgcaatg gccctcctcg atgcggttag tcgcttcgcc gtgcccgctg tttcaggttt
     3601 tctgcaagca c
//
LOCUS       DASUEG010000383         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_654117, whole genome shotgun sequence.
ACCESSION   DASUEG010000383 DASUEG010000000
VERSION     DASUEG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654117"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VFX11_07065"
     CDS             <1..1282
                     /locus_tag="VFX11_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX4938417.1"
                     /translation="IVHLGERDCSIQRRHQKVVEEAPSPAVNAELRARMGAAAVNVAK
                     LVDYVGAGTVEFLLDASGEFYFLEMNTRLQVEHPVTELVTGQDLVEWQLRVAAGEPLP
                     LQQHEIELKGHAIEVRLYAEDPAQNYLPQTGPVHFWHWEDLPGTRADHGVRVGSDISP
                     FYDSMLAKMIACAPDRSTAIRKLDRLLENTVLLGTTNNKHFLRQLITQPSFMEGGATT
                     AFISQHQQALVPPPAFTPAIAALAAALWARHHSQPGAPNPHRTTPVLLQAGDKQIHLD
                     VIAGAGPTRQVKGPDFAVELQLLDHERNRVTALIDGIRRNGWFHIEGKQLWLDCAGQT
                     LSATIVTHAPVRKADAAGSGRIVAPMDGNVVAVLVEQGQPVSKGTTLAILEAMKMEHP
                     LRADIDGTVDSILVTKGAQLKSRQVMILLKPQAK"
     gene            1568..>2735
                     /locus_tag="VFX11_07070"
     CDS             1568..>2735
                     /locus_tag="VFX11_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938418.1"
                     /translation="MTSSTTDVITWGMMLKKAPFLMRNLPRIIKGLRISNNKDPARPC
                     GLGWTFEQAVKNNPRGSALLYENVRFTYDEVNQWANRYAHYLTSQGIKKGDVVAVFIE
                     NRPELLVTVLAVAKIGAVSAMLNTSQTQKVLIHSFNLVKPKAAIVGEELVESFEEVRD
                     ELEVKPDMVYFVADKDTTRDPGVAPKGYKNLATESANSPTHNPDSTNQIYLNDPCFYI
                     YTSGTTGLPKAGIFRHGRWMKAYGGFGIMAMRLGPKDVMYSTLPLYHATGLCVCWGSA
                     VAGAAGFAIRRKFSASNFWKDARKFNATAIGYVGELCRYLLDQPPQADDANNPVRKMI
                     GNGLRPNVWMPFKERFKIEEICELYAASDGNIGFTNIFNFDNTVGFSPMRWVLVE"
BASE COUNT          585 a          900 c          775 g          475 t
ORIGIN      
        1 catcgtccat ctgggcgagc gcgactgctc catccagcgt cgccatcaaa aggtggtcga
       61 ggaagcgccc tcgcccgccg tgaatgccga actgcgcgcc cgcatgggtg ctgctgcggt
      121 gaacgtggcg aaactggtgg attatgtggg ggccggcacc gtggaattcc tgctggatgc
      181 cagcggcgag ttctatttcc tggaaatgaa cacgcgcctg caggtggagc acccggtcac
      241 cgaactggtc accggccagg atctggtgga atggcaactg cgggtggctg ctggagaacc
      301 gctgccgctg cagcagcacg aaatcgaact caagggtcac gccatcgaag tgcgcctgta
      361 tgccgaagac ccggcgcaga actacctgcc gcagaccggc ccggtccatt tctggcactg
      421 ggaagacctg cccggcaccc gcgccgacca cggcgtgcgc gtcggcagcg acatcagccc
      481 gttctacgat tccatgctgg ccaagatgat cgcctgcgca ccggaccgca gcaccgccat
      541 ccgcaagctc gaccgcctgc tggaaaacac ggtgctgctg ggtaccacca acaacaagca
      601 cttcctgcgg cagctgatca cccagccgtc ctttatggaa ggaggtgcca ccaccgcctt
      661 catcagccag caccagcagg ccctggtgcc accgcctgcg ttcacgccgg ccatcgccgc
      721 actcgccgcc gcactctggg cgcggcacca cagccagccg ggggcaccca atccgcatcg
      781 taccaccccg gtattgctgc aggctggcga caagcagata cacctggatg tgattgccgg
      841 cgccgggcca accaggcagg tcaaagggcc ggacttcgcc gtcgaactcc agctgctgga
      901 ccatgagcgc aaccgggtca ccgccctgat cgatggcatc cgtcgcaacg gctggtttca
      961 tatagaagga aagcagctct ggctcgactg cgccggacag accctcagcg ccaccattgt
     1021 cacccacgcc ccggtacgca aagccgacgc cgccgggtcc ggccgcatcg tcgcgccgat
     1081 ggatggcaat gtcgtggccg tactggtgga gcaaggtcag ccggtcagca agggcacgac
     1141 tctggccatt ctggaagcca tgaagatgga acatcccctg cgggccgata tagatggtac
     1201 tgtagactcc attctggtca ccaaaggggc ccagctaaag tcccggcagg tcatgatcct
     1261 gctgaaaccc caggcaaaat aggctgtgca gggtgtattt ggcgccagaa tccggacggg
     1321 ccggcatacc cctgacgcga cggcgatttc gtccgccaag gcttgaacag ggttgggctt
     1381 gcggttatcg tacgcccgac gcgaagtgga taataagtcg aagttaataa caaaatacgg
     1441 cgtgcttcat tcgtttccct gtcgttgagc gcctggcatt tccaccttca aaaacaccca
     1501 aaaccggccg tcactggatg acttggcttt tgtttcaaat caactggact tcctcgcggg
     1561 gggtagcatg actagcagta ctactgatgt gatcacctgg ggcatgatgc tgaagaaagc
     1621 accttttctg atgcgcaatc tgccccggat aatcaaagga ctccgaatca gcaacaacaa
     1681 ggatccggca cgtccctgcg gcctgggctg gaccttcgaa caggcggtga aaaacaatcc
     1741 ccgcggctcc gcactgctgt atgagaacgt gcgcttcacc tacgacgaag tgaaccagtg
     1801 ggccaaccgc tatgcccact acctcaccag ccagggcatc aagaagggcg acgtggtggc
     1861 cgtgttcatc gagaaccgcc ccgagctgct ggtcaccgta ctggcggtcg ccaagatcgg
     1921 cgccgtatcc gccatgctga acacctccca gacccagaaa gtgctgatcc acagcttcaa
     1981 tctggtgaag ccgaaagcgg ccatcgtggg cgaagaactg gtggaatcct tcgaagaagt
     2041 tcgcgacgaa ctcgaagtca aacccgacat ggtctatttc gtcgccgaca aggacaccac
     2101 ccgtgatcct ggcgtggcac ccaaaggcta caagaacctg gccaccgaat ccgccaacag
     2161 ccccacccac aatccggatt ccaccaacca gatctacctg aacgatccct gtttctatat
     2221 ctacacgtcc ggcaccaccg gtctgcccaa ggcgggcatc ttccgccacg gccgctggat
     2281 gaaagcctac ggcggcttcg gcatcatggc catgcgcctc ggcccgaaag acgtcatgta
     2341 ctccaccctg cccctctacc acgccaccgg cctgtgcgtc tgctggggct ccgccgtggc
     2401 aggtgctgcc ggcttcgcca tccgccgcaa attcagcgcc tccaacttct ggaaggacgc
     2461 gcgcaagttc aacgcgaccg ccatcggcta cgtgggagaa ctctgccgct acctgctgga
     2521 ccagccgccc caggcggacg acgccaacaa cccggtgcgc aagatgatcg gcaacggcct
     2581 gcgtcccaac gtctggatgc cgttcaagga gcgtttcaag atcgaggaaa tctgcgaact
     2641 gtacgcagct tctgacggca acatcggttt caccaacatc ttcaactttg acaacaccgt
     2701 gggcttctcg cccatgcgct gggtgctggt ggaat
//
LOCUS       DASUEG010000384         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_898550, whole genome shotgun sequence.
ACCESSION   DASUEG010000384 DASUEG010000000
VERSION     DASUEG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898550"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="VFX11_07075"
     CDS             <1..1694
                     /locus_tag="VFX11_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4938419.1"
                     /translation="ALRAADLGLSVLIVEKAHKFGGTSATSGGVMWIPNNQLTPSEDS
                     REKTFTYIDSLLRGPVQRDRLEAYVDEAPRMARFLRSLGVPLVQAAWPDYFQTAPEAR
                     ADRSVLCDTFDGRLLDDNQFVAMREQYNRFKIFRRYAMDIGEFFAISTRSPGWIQVFL
                     KMLWRYWSDIGTRMISSRDRRFTQGGALMGHIYQCIFDRGVEVRTETALQELIVTDGL
                     VTGVKVNNTGRLYQINANQGVVLAAGGFEWNQELRDRFFTVPGLTRHSSSPEDANRGE
                     ALLAGMKIGAATEHTEAGWWIPTMHKPMTGASNFEEIHQAAFDVGRPHSVCVNRNGNR
                     FVDEACSYDEFGIAMVEDQKKTGANTPCWLVFDATFRTKFTAGGFLPTMIMPDWRIPA
                     DCWDHYIFRANTLDALAAKIHVPADALRKTVANMNDYARSGIDPEFGRGGNAYDLMFG
                     DANIKPNPCLGPIDRAPFYAVPINLGDLGTKGGLKANAKAQVLDANDKPIPGLYAAGN
                     NAGSPFGNLYPGAGGTIGPAATFGFVAANDIAVRAGKLAQEKPVAAHTRNKEAVV"
     gene            complement(1754..2974)
                     /locus_tag="VFX11_07080"
     CDS             complement(1754..2974)
                     /locus_tag="VFX11_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938420.1"
                     /translation="MSANRGEFRMGWVALLAATAGMSSGLALNAYINNIFGPYLLEAF
                     GWSKSALALNGAISIVTVLWIPFVGRMADLYGVRAVALFGIIGYPCSFILMSLLTGDV
                     RELYAVHFLQVIFCASTTATVYCRVVAEKFSRHRGLALAICAAGPAVAGAIASPLLTP
                     YIIANGWRAGFQAMAVFSGLLGVLTLILLPPKQESRTVTSSQYSARKDYGAIFTNPAF
                     RIIAVSSFLCSLTHALATSQVKIVLADHGVTPVQAGYMVSVFAVGVILGRLIAGVALD
                     KWPTHIVAAIGMGLPFLGSYILATDTAHLILLGVAIILIGLSFGAEADILAYVTARYF
                     PMHIYSSVMGLLTTAVGLAIGLGSGLLSYTLSKTDSFTAFLLFGGTLVLIGSLNLLRL
                     GSMDRANRQDAQAA"
     gene            3144..>4431
                     /locus_tag="VFX11_07085"
     CDS             3144..>4431
                     /locus_tag="VFX11_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938421.1"
                     /translation="MANNKVGAFTPLRHKTFRNIWTASVFSYFGHLILGVGVAWEMTR
                     LTDSPTMVALTQTALMMPYMLVAMMAGAVADMFDRRLIALVSLAFAIIASGTLTTLAF
                     LGITTPWMLLLFTVMIGCGVAFYSPSWQASIPEQVPKEQLPAAIGLGSVAYNVARSAG
                     PALGGVIVMALGANYAFAVNSVFYIPLFVTFYLWHRPTVASRLPPERIGRALVSGVRY
                     AKHSSPIKRALLRGFLVCICIASANALAPLIARDILGGDADIYGVLLGVSGVGAVCAG
                     LAVSHLRSLASTESLLKILALTGAVALLVVGMSRSMLLTCAGLFVASGASILVVSLLN
                     VTVQMSTPRWVMARALSMYQTCTAGGIAIGAWMWGQFAADYSIQTAMIATAAALLATA
                     LFGFLLPLPKDVDEDIEQIVIYNEPKVALDLSLRSGP"
BASE COUNT          970 a         1297 c         1232 g          932 t
ORIGIN      
        1 ccgcactgcg tgctgcagat ctcggcttat ccgtattgat cgtggaaaag gcgcacaagt
       61 tcggaggtac ctcggcaacc tcgggcggcg tgatgtggat ccccaacaat cagctcacgc
      121 ccagtgaaga cagccgggaa aaaacattta cgtatatcga cagtctgttg cggggaccgg
      181 tacaacgcga ccggctggaa gcgtacgttg acgaagcgcc cagaatggca cgcttcctga
      241 gatcactggg tgtgccgctg gtacaggcgg cgtggcccga ctatttccag actgcgccgg
      301 aagcgcgcgc cgaccgatcc gtgctctgtg atacctttga tggccgcctg ctggatgaca
      361 atcagttcgt cgccatgcga gagcagtaca atcgcttcaa gatttttcgt cgttacgcca
      421 tggatatcgg cgagttcttt gccatatcca cccggagccc cggctggatc caggtcttcc
      481 tgaaaatgct gtggcgctac tggtcagaca ttggaacccg catgatctcc agccgcgatc
      541 gccgcttcac tcagggtggc gcgctgatgg gtcacattta tcaatgcatt ttcgaccgtg
      601 gcgttgaagt tcgcaccgaa actgccctgc aggagttgat cgtcaccgac ggcctcgtca
      661 cgggagtcaa agtcaacaat acgggtcggc tgtatcagat caatgccaat cagggcgtag
      721 tgctggccgc aggcggtttt gaatggaacc aggaactgcg ggaccggttt ttcacggtac
      781 cgggactcac ccgccacagc agttcaccgg aagacgccaa tcggggtgaa gccctgcttg
      841 ccggcatgaa aattggcgct gcaacggaac acaccgaagc aggctggtgg attcccacca
      901 tgcacaagcc catgacaggc gcgtccaatt tcgaggaaat ccatcaggct gcatttgacg
      961 ttggccggcc acacagcgtc tgcgtaaacc gcaatggcaa ccgctttgtg gacgaagcct
     1021 gcagctacga tgaatttgga attgccatgg tggaggatca gaaaaaaacc ggcgccaaca
     1081 cgccgtgctg gctggtattc gatgcgacat tccgcaccaa gtttactgcc ggcggcttcc
     1141 tgccaaccat gatcatgccg gattggcgaa ttcccgccga ttgctgggat cattatattt
     1201 tcagggcaaa taccctggat gccctggccg ccaaaattca tgtgccagcg gacgcactgc
     1261 gaaaaacggt cgccaacatg aacgactacg cccgatccgg aatcgatccg gaattcgggc
     1321 gcggaggcaa tgcctacgat ctgatgttcg gcgacgccaa tataaaaccc aatccctgtc
     1381 ttggtccgat cgacagggcg ccgttctacg cggtgccgat caatctgggg gatctgggta
     1441 ccaagggtgg cctcaaggcc aacgccaaag cccaggtgct ggatgcgaat gacaaaccca
     1501 tcccgggttt atatgccgcc ggcaacaacg ctggcagccc gtttgggaat ctctatcctg
     1561 gcgcgggtgg cacgattggt ccggcggcaa cctttgggtt tgtggcagcg aacgacattg
     1621 cagtgcgagc cggcaagctc gcacaggaaa aacccgttgc tgcgcacacc aggaacaaag
     1681 aggccgtggt ttaaggatag tgttcctggg gaatcacatg cagtcatgtc catggctgca
     1741 agtcgacgtg ccgtcacgct gcttgtgcat cctgccggtt tgcccggtcc attgaaccca
     1801 ggcgcagcag gttcaggctg ccaatcaaca ccagcgtgcc cccaaatagc aggaaggcag
     1861 taaaactgtc cgtcttcgag agcgtgtaac tcagcaatcc tgaaccgagc ccaatggcca
     1921 gcccgactgc cgtcgtcagc aatcccatta cagagctgta gatgtgcatg gggaaatatc
     1981 gtgccgtcac gtaggcaaga atatcggcct cggcaccaaa ggaaaggccg atcaggataa
     2041 tcgccacgcc cagcagaatt aagtgtgcag tgtccgttgc cagaatatag ctgcccagaa
     2101 agggcaaacc catgccaatg gcagctacga tatgggtggg ccatttatcc agcgcaactc
     2161 ccgcaatcag tctgcccaga atcacgccca ccgcaaaaac cgaaaccatg tagccggctt
     2221 gaactggcgt tacaccgtga tccgccagga cgattttgac ctgcgatgtc gccagggcat
     2281 gggtcaaact gcaaaggaat gaactcaccg caataatccg aaacgccgga ttcgtgaaga
     2341 tcgccccgta atccttgcgc gcgctgtact gtgacgaggt gacagtgcga ctttcctgtt
     2401 tcggcggcag caggatcagg gtcaatacac ccagcaatcc ggaaaatact gccatcgcct
     2461 gaaaccccgc cctccagccg ttggcgatga tatacggtgt gagcaaagga ctcgcgatcg
     2521 cacctgccac cgcaggaccc gccgcgcaaa tggccagcgc cagcccgcga tgccgcgaga
     2581 atttctctgc cactactctg caatatacgg tggcagtcgt ggacgcgcag aaaatgactt
     2641 gcagaaaatg aacggcgtac aactcccgga catcccccgt cagcaggctc atcaagataa
     2701 aactgcacgg atatccgatg atgccgaaaa gcgccacagc gcgaaccccg taaagatcgg
     2761 ccatcctccc gacaaacggt atccacagga cagtaacgat ggagatggca ccattcagtg
     2821 ccaaagcaga tttgctccag ccaaaagctt ccagcagata tgggccaaaa atattgttta
     2881 tgtaagcatt cagagccagg ccggaactca tgcctgcggt tgcagcaagc aacgcaaccc
     2941 agcccatacg gaattcacca cgattggcgc tcattatctg tttcctgtgc tcatggaaca
     3001 cgaaaagatg aatttcacaa tgttgaatga cgtgattttc aggccggggt gcctgccagg
     3061 catcaagcca gacgtggcgc tattatttaa tcatcattgc tgtatattat aaaaccctgc
     3121 cctctacttt tattgattct gccgtggcca acaacaaggt cggtgccttt acacccctca
     3181 ggcacaaaac tttccgcaac atctggaccg ccagtgtttt ctcgtatttc ggtcacctca
     3241 ttctgggcgt gggcgtggcg tgggaaatga cccgtctgac cgattcccct accatggtgg
     3301 ccctgaccca gaccgccttg atgatgccct atatgctggt ggccatgatg gcgggcgccg
     3361 tggccgacat gttcgatcgc cgcctgattg cgctggtgtc actggcattc gccatcatcg
     3421 cgtctggcac gttgaccacg ctggcatttc tcggcatcac cacaccctgg atgctgttgc
     3481 tcttcaccgt gatgattggc tgtggcgtgg cattttattc cccgtcctgg caggcctcga
     3541 ttccggagca ggtgccaaag gaacaattgc ccgccgcgat cggactgggc agcgttgcct
     3601 acaatgtggc gcgcagcgca gggccggcct tgggcggcgt catcgtgatg gcccttgggg
     3661 cgaactatgc ctttgcggtg aattcggttt tctacatccc gttgtttgtc acgttttatc
     3721 tctggcatcg ccctacggtt gcatcgcgct tgccaccgga gcgcatcggc cgggcgctgg
     3781 tatccggtgt gcgctacgca aaacactcat cccctatcaa gcgcgcgctg ctgcgcggct
     3841 ttctggtctg catctgcatc gcgtcggcca atgctctggc accgctaatt gcgcgggata
     3901 ttctgggagg tgacgccgac atctacggcg tgttactggg tgtcagcggc gtaggcgcgg
     3961 tctgtgccgg acttgcagtg agtcacctgc gttcgctggc atcgacagaa agcctgctga
     4021 aaatcctggc cttgaccggc gctgtcgccc tgcttgttgt gggaatgagc cggtccatgc
     4081 tgctgacctg tgccggatta tttgttgcca gcggcgccag cattctcgtg gtatcgctgt
     4141 tgaacgttac cgtgcagatg tcaacaccac gctgggtgat ggcgcgcgca ctgtcgatgt
     4201 atcaaacctg cacggccggc ggcatcgcca tcggcgcctg gatgtggggc cagttcgccg
     4261 ccgactattc cattcagacc gccatgatcg ctactgccgc ggcattgctc gccactgcgc
     4321 ttttcggctt tctgctaccg ctgcccaaag atgtcgatga agacatcgag cagatcgtca
     4381 tttacaatga accgaaagtg gcactggatt tatctttgcg ttccggcccc a
//
LOCUS       DASUEG010000385         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_224211, whole genome shotgun sequence.
ACCESSION   DASUEG010000385 DASUEG010000000
VERSION     DASUEG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224211"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VFX11_07090"
     CDS             <1..1357
                     /locus_tag="VFX11_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938422.1"
                     /translation="ALYPALAEQATALALEAQLGLKDHHGALRHSPLALLPWQISRDD
                     FMQAQQVARAIGARLDRLARDNAALAQALGPVLEGRTLQAQLWQALQSVPLAQRRARP
                     LNMTRVDLLQDQARKWKLVEANSIAAGMGPFSEGLAQIQQQLWPELQRLGLATQGRPA
                     FAANPVTEALGDTLYQAAVGQLRQHPSTHPDAVAPVVVFVVEPAEDNIFDQRKVARVV
                     EQKGARVVRLTLQEIDALLERSLAPACHLRGIGPVQVLYFRTGYNLADFQDAHGRSER
                     LLRLRAELETLQVTLAPTIGLQLASAKAVQVHWYRQPEADAATEALDTAQFFVADPQV
                     QDVVNWPQWVLKSQGEGGGNVVQDEAIPARIATLSQQERREWLLMRKIPLVPRQSLVP
                     VLRNGAIQFAQQLMSELGLFVLGDDVRIGGYLLRSKPVAALETGVHRGQGMIDTVAFS
                     D"
     gene            complement(1347..2330)
                     /locus_tag="VFX11_07095"
     CDS             complement(1347..2330)
                     /locus_tag="VFX11_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938423.1"
                     /translation="MSRLDSYFVADLDDPGDWTFQPILTAEQQRQLLRFYTTTANVLR
                     HRLQRELGATARAFDLEGFLRSLMPAHCNEDDIRMAQRLLMIQEYSAHRGHAPAMATG
                     GTVSTRNQTRWKRWQPWLQQQLLAAAQQNAEQSLLWRCHLVRVPQQWLGASAIAAGMR
                     QQLQASRALQSLQIEELQLELQPPAPGGLDWIEQAHYQELDRISAELAQERTVIIELI
                     RDGSQLQAFSDLALAFELDSRSALETSLHYTHPTLGQQTLQLAMGENAIRVIADSALQ
                     RGLADCSVKAFRLWRCAGSTAPPWFGVRRYVPTLIPWSVFWWLRHALKLNR"
     gene            complement(2327..2617)
                     /locus_tag="VFX11_07100"
     CDS             complement(2327..2617)
                     /locus_tag="VFX11_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938424.1"
                     /translation="MNQDIETRTQLAAGTIPCPFCQTPIILDLQSIFAARPLRCSHCD
                     AELQLNRESSAAALQQLQNWKDDMEQAQQPLASTAEEPVMIPRPRKPRRQRT"
     gene            complement(2614..3252)
                     /locus_tag="VFX11_07105"
     CDS             complement(2614..3252)
                     /locus_tag="VFX11_07105"
                     /inference="COORDINATES: protein motif:HMM:NF023085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HEX4938425.1"
                     /translation="MADEDESKKDSALSLTQLIGAPIRALVEAEAQAAMATANFIRSV
                     GFVQDGSGNFGKLGDLQMASFLRKRRNAQGEEETVEVQIPLLTMLPIPALQIRDAELE
                     YTVKILDTELSRSDSPAAQQASALGMPDVPATLRATLARDTRSEHRRSLDMLVKMKVN
                     IEQSDMPAGLAKLLSISAESVNQRLVQESEPAPASGSATAKPALPSSGDFTI"
     gene            complement(3259..4068)
                     /locus_tag="VFX11_07110"
     CDS             complement(3259..4068)
                     /locus_tag="VFX11_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938426.1"
                     /translation="MADLQQLIGAILRDLAKARFASDLYSRSVARYYENDFLLRRFPI
                     PRSEIEEADLELKFAIADVRDNGVNTETREANIAFVLERTVEELVAIFLDCAGVFVDH
                     PDQRDVKEDLHSKLSKGFNSIAMRIELRQLALRYFIDSYTHLIGLKGQFDYEMALKGL
                     HSPFFWGITNYRKEGVNLSDLNAPVDAVINAVFAREEFTEVLKDLAGPIQKIWQDNHD
                     ARLEVEVSGDRLMHLGEGTLSTLKLKMSVRNHMWTEVKTEEHRSTRVLTSE"
     gene            complement(4110..>4613)
                     /locus_tag="VFX11_07115"
     CDS             complement(4110..>4613)
                     /locus_tag="VFX11_07115"
                     /inference="COORDINATES: protein motif:HMM:NF023085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HEX4938427.1"
                     /translation="AAMSSVKFIKAVGFKENGDVNNISFTAKKGDQSVEITVPLLTIV
                     PIPFIRIDDMTINFKTNITQSEEAKDESSSSFAASASLEASARYLFFSAKLTGSISNK
                     KDSSSTKDSKYSVEHTMDVTVHAVQDDMPAGLARMLSILTKSIDDMGAPAAVPGKGGK
                     GGKNNGE"
BASE COUNT          772 a         1369 c         1534 g          938 t
ORIGIN      
        1 cgccctctac cctgcgctgg cagaacaggc cacggccctg gcgctggaag cgcaactggg
       61 cctgaaggac catcacggtg ccctgcggca cagcccgctg gcgctgctgc cctggcagat
      121 cagccgcgat gatttcatgc aggcgcagca ggtggcccgg gccatcggtg cccggctgga
      181 tcggctggca cgggacaacg ccgccctggc gcaggccctg gggccggtgc tggagggccg
      241 gaccctgcag gcccagctgt ggcaggcgtt gcagtcggtg ccgctggcgc agcgccgggc
      301 gcggccgctc aacatgaccc gcgtcgacct gctgcaggat caggcgcgga agtggaagct
      361 ggtggaggcc aacagcatcg ccgctggcat gggacctttc agcgaagggc tggcgcagat
      421 ccagcagcaa ttgtggccgg aactgcagcg gctgggtctg gcgacacagg gccgtcctgc
      481 gttcgcggcc aatcctgtca ccgaagcgct gggggacacc ctgtaccagg ccgcggtcgg
      541 gcagctgcgc cagcacccgt ccacccaccc ggacgcggtg gctcccgtcg tggtcttcgt
      601 ggtggaaccc gccgaggaca atattttcga tcagcgcaaa gtggcgcgcg tggtggagca
      661 gaaaggagcg cgcgtcgtgc ggctcacctt gcaggaaatc gatgcgctgc tggagcggtc
      721 cctggcgccg gcctgccacc tgcgtggcat tggtccggtg caggtgttgt atttccgcac
      781 cggctacaac ctggcggatt tccaggatgc gcacgggcgc agcgaacgcc tgctgcggtt
      841 acgtgcggaa ctggaaaccc tgcaggtcac gctggcgccc accatcggtc tgcaactggc
      901 atcggccaag gccgtgcagg tgcactggta ccggcagccg gaggcagatg ctgccacgga
      961 ggcgctggac acggcgcaat tttttgtcgc tgatccgcag gtgcaggatg tggtgaactg
     1021 gccgcagtgg gtactgaaaa gccagggcga aggcggtggc aacgtggtgc aggatgaggc
     1081 cattcccgcg cgcattgcca cgctgtcgca acaggagcgg cgcgaatggc tgctgatgcg
     1141 gaaaattccg ctggtgccgc gtcagtctct ggtgcctgtt ctgcgcaatg gcgcgatcca
     1201 gtttgcgcaa cagctgatgt cggaactggg cctgttcgtg ttgggggatg acgtgcgcat
     1261 cggtggttac ctgctgcgca gcaaaccggt ggcagcgctg gaaaccggtg tgcaccgggg
     1321 gcagggaatg attgatacgg tggcgttcag cgattgagtt tcagcgcgtg gcgcagccac
     1381 cagaaaacac tccacggaat cagcgtgggc acgtaacggc gcacgccaaa ccagggtggc
     1441 gccgtgctgc cggcgcagcg ccacaggcgg aaggctttca ccgagcagtc ggccaggccg
     1501 cgctgcaggg cggaatccgc gatcacccgg atggcatttt cgcccatcgc cagttgcagt
     1561 gtctgctgcc ccagtgtcgg gtgagtatag tgcaggctgg tctccagggc agaccggctg
     1621 tccagttcga aggccagggc cagatcggag aatgcctgca gctgactgcc atcgcggatc
     1681 agttcgatga tgacggtgcg ttcctgcgct aactcggcgc tgattctgtc cagttcctga
     1741 tagtgcgcct gctcgatcca gtccagcccg ccgggtgccg gcggctgcag ttccaattgc
     1801 aattcctcga tctgcaacga ctgcaatgca cggctcgcct gcagttgctg ccgcatgccg
     1861 gcggcgatgg cgcttgcgcc cagccactgc tgcggcaccc gcaccagatg gcagcgccag
     1921 agcaggctct gttccgcatt ctgctgcgcc gctgccagca attgctgctg cagccagggc
     1981 tgccagcgtt tccagcgcgt ttgattgcgg gtggacaccg taccgccggt cgccatcgcc
     2041 ggtgcgtggc cccggtgcgc cgaatattcc tggatcatca gcaggcgctg ggccatgcgg
     2101 atgtcatctt cgttgcaatg ggccggcatc aggctgcgca gaaacccttc cagatcgaag
     2161 gcgcgggcgg tggcgcccag ttcccgttgc agacggtgcc gcaggacgtt ggccgtggtg
     2221 gtgtaaaaac gcagcagctg gcgttgctgt tccgcggtaa gaatcggttg gaaggtccag
     2281 tcgcccggat cgtcgaggtc ggcgacgaaa taggaatcca gtctgctcaa gtgcgctggc
     2341 gccggggttt gcgcggacgt ggaatcatca ccggctcctc ggcagtggac gccaggggct
     2401 gctgggcctg ttccatgtcg tctttccagt tttgcagttg ctgcagggcg gcggcggacg
     2461 attcccggtt gagttgcagt tcggcatcgc aatgggagca gcgcaacgga cgtgccgcga
     2521 agatcgattg caggtccaga atgatcggcg tctggcagaa ggggcacggt atggtgcccg
     2581 ccgccagctg ggtgcgggtt tcgatgtcct ggttcatatg gtgaagtcgc cgctggatgg
     2641 tagtgcaggt ttggcggtgg ctgagccgga tgcgggtgca ggctcggatt cttgcaccag
     2701 acgctggttg acgctctccg ccgatatgct cagcagtttg gccagtcccg cgggcatgtc
     2761 cgactgttcg atgttcacct tcattttcac cagcatgtcc agcgaccgcc ggtgctcgct
     2821 gcgcgtgtcc cgggcgagag tggcgcgcag ggtggcgggc acatcgggca tgcccagtgc
     2881 gctggcctgc tgcgcggccg ggctgtcgct gcggctcagt tcggtgtcga ggattttcac
     2941 ggtgtattcc agctccgcat cgcggatctg cagggcggga atcggcagca tggtgagcag
     3001 gggaatctgc acctcgaccg tttcctcctc gccctgcgca ttgcggcgct tgcgcagaaa
     3061 actggccatc tgcagatcgc ccagcttgcc gaaattgccg gaaccatcct gcacgaaacc
     3121 cacgctgcgg atgaaattcg cggtggccat ggctgcctgc gcttcggctt ccaccagggc
     3181 gcgaatgggg gcgccgatca gctgggtcag gctcagtgcg ctgtctttct tgctttcgtc
     3241 ttcatcggcc atgatccatc actccgaggt gagtacgcgt gtggaacggt gttcttccgt
     3301 tttcacttcc gtccacatgt gattgcgcac ggacattttc agcttcaggg tggacagggt
     3361 gccttcaccc aggtgcatca gccggtcgcc cgacacttcc acttccagcc gggcatcgtg
     3421 gttgtcctgc cagattttct ggatcggccc cgccaggtct ttcagcactt cggtgaattc
     3481 ctcccgcgcg aacacggcat tgatcaccgc gtcgaccggt gcgttcaggt cgctcaggtt
     3541 cacgccttcc ttgcggtagt tggtgatgcc ccagaagaac ggactgtgca gccccttgag
     3601 cgccatctca tagtcgaact ggcccttgag gccaatcagg tgagtgtagg aatcgatgaa
     3661 atagcgcaac gccagctgcc gcagctcgat gcgcatggca atggaattga agcctttgct
     3721 cagcttgctg tgcaaatcct ctttgacatc gcgctggtct ggatggtcca caaacacgcc
     3781 ggcgcaatcg agaaatattg ccaccagctc ttccaccgtg cgttccagca caaaggcaat
     3841 gttggcctcg cgggtttccg tattcacgcc gttgtcgcgc acgtcggcga tggcgaactt
     3901 cagctccaga tccgcctctt cgatttcaga gcggggaatc ggaaagcgtc gcagcaggaa
     3961 atcattttcg taatagcggg caaccgagcg ggaatacagg tccgaggcga agcgcgcctt
     4021 ggccagatcc cgcagaattg ccccgatcag ttgttgaagg tcggccatcg gttccccctt
     4081 gtgctggcct tgttctggca gggcggcggt tattcgccgt tattcttgcc gcccttgcca
     4141 cctttgccag ggacggcagc tggcgcgccc atgtcgtcga tggatttggt caggatcgac
     4201 agcatgcgtg ccagtccggc cggcatgtca tcctgcactg catggaccgt gacgtccatc
     4261 gtgtgctcca cagaatactt cgaatccttg gtggagctgg aatccttttt gttggaaatg
     4321 gagccggtca gtttggcaga gaagaacagg taacgggcgc tggcttccag gctggcgctg
     4381 gcggcgaaag acgagctgct ttcgtccttg gcttcttctg actgggtgat attggttttg
     4441 aagttgatgg tcatgtcatc gatgcggatg aagggaatcg gcacgatggt gagcaacggt
     4501 accgtgattt caacgctctg gtcgcctttc ttggcggtga agctgatgtt gttcacgtcg
     4561 ccgttttcct tgaagcccac ggccttgatg aattttaccg aggacatggc ggc
//
LOCUS       DASUEG010000386         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1086038, whole genome shotgun sequence.
ACCESSION   DASUEG010000386 DASUEG010000000
VERSION     DASUEG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086038"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VFX11_07120"
     CDS             complement(<1..1234)
                     /locus_tag="VFX11_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938428.1"
                     /translation="MFRRVRKRLNKLYQTRYATYRELLQPAADWLLPRLPRRVVAGAP
                     WWWRWLGLYWLLAFLALHCSGHDVQLTAELRRAFGSTRRKYIAYLLGDRLLRHRDYTE
                     VTQQLFRRATWRNCEIHANFRYIDFVVQVKGFRTAQLDLMLRRLSGKRLLPHLTRYQK
                     TRLLIHFLQKNNARKISDISRQVGARYLDKFSGPLYLMHQSVRRGWVSDPHTQMGSEM
                     YRQLVAARSRFEQVIRDNASSFCVVGNAPCELGLGKGPLIDSFNIVIRLNDYSTEEVA
                     DYGSRQTIWVRVANREVVRHHAPANQLVVFSANNFAVKRRDAYQYLLPLYLARKEFTS
                     IPNHVYQDLIRQLGGLPSTGLAILYWIYKIIGPIPRRQIFGFTHLSDGSDFAAHYYKD
                     GVEIGVHLHEWQRETLLMQ"
     gene            complement(1267..2172)
                     /locus_tag="VFX11_07125"
     CDS             complement(1267..2172)
                     /locus_tag="VFX11_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938429.1"
                     /translation="MAFKHVVATLMLVMAPWLAHARTVAGVADIQGEKPRYLQQVSMD
                     FDNAAAVQQALFVPALDDGFIPQGLAQRKGAFYVSGYVQARDGQRPECRIYRLLKDKP
                     RERILLPLPASCNHAGGLAMTDGGVLLISDNIRLFTLLVSDGGKPLKRAAREIRLQAP
                     LKGTFVSYHRGMVYLGSYMKDLDVTRVHVFPQSVLSKAEISVKDAVDSFDAPHKSQGA
                     YFDDSDYAWTTSQAKGFNYLNKFNMKTRKTEQRYRLVRGMQGIVKDADGSLWVVSEAG
                     ALKYRDWSQRFPAIFRLDVKRLQAE"
     gene            complement(2233..>2393)
                     /locus_tag="VFX11_07130"
     CDS             complement(2233..>2393)
                     /locus_tag="VFX11_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HEX4938430.1"
                     /translation="SVDAFVIGDDWTGHFDFLKPYCEVYYLPRTQSISTTEIKQQIAS
                     GGRRLRSA"
BASE COUNT          520 a          726 c          680 g          467 t
ORIGIN      
        1 cttgcatcag cagggtttca cgctgccatt cgtgcaggtg aacgccgatt tccacaccat
       61 ctttgtagta atgcgccgca aaatccgacc catcggacag atgggtaaac ccgaaaatct
      121 gccggcgcgg aatggggcca ataatcttgt aaatccagta caggatggcc aggccggtgc
      181 tgggcaagcc gcccagctgc cggatcagat cctgatacac atgattggga atggaggtaa
      241 attccttgcg cgccagatag agcggcaaca ggtactggta ggcatcgcgg cgttttacgg
      301 caaagttgtt cgccgagaac accactagct ggttggccgg agcgtggtgg cgcaccacct
      361 cgcgattggc cacgcgcacc cagatggtct gacggctgcc gtaatcagcc acctcttccg
      421 tgctgtagtc attcagacgg atcacaatat tgaagctgtc gatcagtggc cccttgccca
      481 gtcccagctc gcacggcgca ttgcccacca cgcagaaact gctggcgttg tcgcggatga
      541 cttgctcgaa acggctgcgg gcagccacca gctggcgata catctccgag cccatctgcg
      601 tgtgcggatc gctcacccag ccacggcgca cgctctgatg catcagatat aggggaccgg
      661 aaaatttgtc cagatagcgc gcgcctacct ggcggctgat atcgctgatc ttgcgcgcat
      721 tgtttttctg caggaaatga atgagcaagc gggttttctg atagcgcgtc aggtgcggca
      781 gcaagcgctt tcccgacagg cgtcgcagca tcagatccag ctgcgcggtg cgaaagcctt
      841 ttacctgcac gacaaagtcg atatagcgga agttggcgtg aatctcgcag ttacgccagg
      901 tggcccgccg gaacagttgc tgcgtcacct cggtatagtc ccggtgacgc aacagccggt
      961 cgccgagcag gtaggcaatg tatttgcgtc gggtcgagcc aaaagcacgg cgcaattcgg
     1021 cggtcagctg cacatcatgc ccgctgcagt gcaaggccag aaacgccagc agccagtaca
     1081 ggcccagcca gcgccaccac cagggcgcac cggctaccac ccgccgcggc aggcgcggca
     1141 gcagccagtc ggcggcaggc tgcagcagct cacggtaagt ggcatagcgc gtctgataca
     1201 gcttgttcag acgcttgcgg actcggcgaa acacaacatc tccctgtcag cggctcgacg
     1261 gccgcctcat tctgcctgca ggcgcttgac gtccagccgg aaaatggccg gaaaacgctg
     1321 cgaccagtcg cgatacttga gcgcgccggc ctcggatact acccatagtg agccgtcggc
     1381 atctttcacg atgccctgca tgccccgcac cagacggtaa cgctgttccg tcttgcgcgt
     1441 tttcatattg aatttattga ggtaattgaa gcccttggct tgcgaggtgg tccaggcgta
     1501 atccgaatca tcaaaatatg caccctgcga tttatggggc gcatcaaagc tgtccaccgc
     1561 gtccttgaca ctgatttccg ccttgctcag cactgactgc ggaaatacgt gcacgcgagt
     1621 gacgtccaga tctttcatat aactgcccag atacaccatg ccgcggtgat agctgacaaa
     1681 cgtgcccttg agcggcgcct gaagacggat ttcccgggcg gcacgcttga gcggcttgcc
     1741 accatccgac accagcaggg taaacagacg gatattgtca ctgatgagca atacgccgcc
     1801 atcggtcatg gccagaccgc cagcgtgatt acagctggcc ggcaatggca gcaggattcg
     1861 ttcgcgcggt ttgtccttga gcaggcgata aatccggcac tccgggcgct gtccgtcgcg
     1921 cgcctgcaca taaccggata cataaaacgc gcccttgcgc tgtgccagcc cctgcggaat
     1981 aaaaccgtca tccagcgccg gtacaaacaa ggcctgctgc accgcggccg cattgtcaaa
     2041 atccatgctg acctgctgca gatagcgcgg tttctctccc tgtatgtcag caacaccggc
     2101 taccgtacgg gcatgcgcca gccacggggc cattaccagc attaaagtcg ctactacgtg
     2161 tttaaacgcc attgcctacc tcatcgtcat tgacagggcg cacagccgtg ctggccgtgc
     2221 aactgcggtt actcaagccg agcgcaggcg ccggccaccg gaggcgatct gctgtttgat
     2281 ctcggtggtg gagatgctct gcgttcgagg cagataataa acttcacagt agggcttgag
     2341 gaaatcgaaa tgaccggtcc agtcatcacc gataacgaag gcatccaccg aat
//
LOCUS       DASUEG010000387         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_620291, whole genome shotgun sequence.
ACCESSION   DASUEG010000387 DASUEG010000000
VERSION     DASUEG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620291"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFX11_07135"
     CDS             complement(<1..1296)
                     /locus_tag="VFX11_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX4938431.1"
                     /translation="MSLQNRIARLAVTVAQHSSAGAKPRNEDSIAIQIPQDAQLTTKG
                     IVVAIADGVSAAEAGREASEIAVKGFINDYYSTPDSWSVKQSAHKVLIALNRWLYGQG
                     QKYLEAEKGYVTTFSALILKSQAAYVFHVGDSRIYRLRDGVLEQLTRDHVARISADQC
                     YLARALGIDVNLDVDLKVLDLQRGDLFLLTTDGVHEKIEPRLLQQLLLDGERDLDALA
                     QTLVQTALDNGSTDNLSCQLVRIQQLGQETNEDVFRTLHQLPFPPDLSVGMKLDGWRV
                     QRQLHASTRSQLYVVQHEASGQRAVMKTPSRNFIDDPAYIERFLMEEWVGRRIDSPYV
                     VKVVEPPAARTFLYYLTEFVEGPTLAQWIAQRKSTDVTAVVALAEKMAQGLRAFHRRE
                     TLHQDIKPDNVVLRGDDPVIIDFGSVYVAGVDEIATTFAR"
     gene            1725..2090
                     /locus_tag="VFX11_07140"
     CDS             1725..2090
                     /locus_tag="VFX11_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX4938432.1"
                     /translation="MNTFTRTAAIVIPVLAAVAGCNTMEPPEAELVQAKTQVQSADSA
                     EGEQYAALSMKRAQEKIEQAEKAMEEKEYLKAQRLAQQAAADARLAEATAQTEKMKQA
                     LEEVNKNLDALKIELDAQS"
     gene            2122..>2857
                     /locus_tag="VFX11_07145"
     CDS             2122..>2857
                     /locus_tag="VFX11_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4938433.1"
                     /translation="MKPLYPVIIGSVLLTAGCASNEPPAELQSLKQQVTQMQQDPTVN
                     QFAAVELDDAKKQVETAEKAWSSDDDAAYEHQRYLAETQLGLTRQRAELGSIEHQLKE
                     AESRRSDLLLSARKQEVTEARSAAASAEEQLKAIQSEMQNVKAEQTRRGLVLTLQDVL
                     FEVDKAQLQPGGRRTIQTLAEFLRDNPATNVTVEGFTDAQGTDQYNLALSQQRAQAVV
                     DELTSAGIARERMAVKAYGEQYPVASN"
BASE COUNT          668 a          871 c          807 g          511 t
ORIGIN      
        1 gcgggcaaag gtggtggcga tctcgtccac acccgccaca tacaccgaac caaaatcgat
       61 gatcaccgga tcgtcgccgc gcagcaccac gttgtccggc ttgatatcct ggtgcaaggt
      121 ttcgcggcgg tggaacgcac gcagaccctg cgccattttt tctgccagcg ccaccacggc
      181 ggtcacatcc gtgctcttgc gctgggcgat ccactgcgcc agcgtggggc cttccacaaa
      241 ctctgtcagg tagtaaagaa acgtgcgcgc cgccggcggc tccaccacct tcaccacgta
      301 agggctgtcg atgcggcgac cgacccactc ttccatcagg aaacgctcga tgtaggccgg
      361 atcgtcgatg aaattgcgcg acggcgtttt catcactgcg cgctggccgc tggcttcgtg
      421 ctgcaccaca tacagctggc tgcgggtact ggcatgcaac tgccgctgca cgcgccagcc
      481 atccagcttc atgcccacac tcagatccgg tggaaacggc aactgatgca gcgtgcggaa
      541 cacatcctcg ttggtttcct gccccaactg ttggatgcgc accagctggc atgagaggtt
      601 gtcggtgctg ccgttgtcca gcgccgtctg caccagggtt tgcgccagcg catccagatc
      661 gcgttcgcca tccagcagca gttgctgcaa caggcgcggc tcgatttttt cgtgcacgcc
      721 atcggtggtc agcaggaaca gatcgccgcg ctgcagatcc agcactttca gatccacatc
      781 cagattcaca tcgatgccca gtgcgcgggc gagatagcac tggtcggcag aaatacgcgc
      841 cacgtgatca cgggtgagct gctcgagcac accgtcccgc aaacggtaga tacgcgaatc
      901 gcccacatga aacacatagg cggcctggga tttcaggatc agcgcgctaa aggtggtgac
      961 atagcctttt tccgcctcca gatacttctg gccctgcccg tacagccagc ggttgagcgc
     1021 gatcagcacc ttgtgcgccg actgcttcac gctccaggaa tccggcgtgg aatagtaatc
     1081 gttgatgaaa cccttgacgg cgatttccga tgcttcgcgc cccgcctccg cggcactgac
     1141 accatcggcg atagcgacca cgatgccctt ggtggtgagc tgcgcgtcct ggggaatctg
     1201 gatcgcaatg ctgtcctcgt tgcgcggttt ggcaccggca ctggagtgtt gcgccacggt
     1261 aacagcgagc ctggcaattc ggttctgtaa cgacatcgaa gtcaattctg cactcactca
     1321 attcaaaaaa taaaaagact gtgcggtgat acagcaaagc agttggcact acaactgtcg
     1381 aatacggccg caggcattct agcgaagccc ggtcatgcgt caacgcaatt gcagtccagg
     1441 ttcaacgttg ccgacacacg gtttgccggg ccccgcccat caggttaccg ctctgcaccc
     1501 acagccactt gcaaaatggg atacgccagc tgcaaatctt acagaattga ctgcatgcgc
     1561 tgtccattgc gcgcgccgac tgtccggaac ctgtcactga taacgtttat taaatcaatg
     1621 attaatcttg cgcgaacgga tcttcaccgg cacgcgtctg ataactggac tgaaaattga
     1681 atcccgtgct gtgtcacacc ctcaccatag accaggaggt ctggatgaac acctttaccc
     1741 gaaccgcagc aattgtcatt ccggtgctgg ccgcggtggc cggctgcaat acgatggagc
     1801 cacccgaggc tgaactggtc caggccaaaa cgcaggtgca gagcgctgac agcgcagagg
     1861 gcgaacagta tgcggcgctg tccatgaagc gggcccagga aaaaatcgaa caggctgaaa
     1921 aagccatgga ggaaaaagaa tacctgaagg cacaacgact ggcacagcaa gccgcggcgg
     1981 atgcccgact ggcagaagcc accgcgcaaa ccgagaaaat gaaacaggcg ctggaagaag
     2041 taaacaaaaa tctcgacgcg ctgaaaatcg aactggatgc gcaatcctga tgccatgtcc
     2101 tgaagggaag gagaatatct catgaaaccg ctataccccg tcatcatagg cagcgtcctg
     2161 ctgactgccg gctgtgccag caacgaaccg cctgccgaac tgcaatcact gaaacaacag
     2221 gtcacgcaga tgcaacagga ccccaccgtc aaccagttcg ctgccgttga actggacgat
     2281 gcaaaaaagc aggtcgagac tgccgaaaag gcctggtcca gcgacgacga cgcagcctat
     2341 gagcaccagc gttatctcgc ggaaacgcag cttggcctca cccgccagcg cgccgaactg
     2401 ggcagcatcg agcatcaact gaaagaggcg gaaagccgcc gctcggatct gctgttgtcc
     2461 gcccgcaagc aggaagtgac cgaggccagg tctgctgctg cgtccgcaga agagcagctc
     2521 aaggcgatac aatcggaaat gcagaatgta aaagccgagc agacccgacg cggtctggtt
     2581 ctgacgctgc aggatgtcct gttcgaagtg gacaaggccc agttgcaacc cggcggtcgt
     2641 cgcaccatcc agacgctggc ggaattcctg cgggataatc cggccaccaa cgtcacggtg
     2701 gaaggtttca ccgatgccca ggggacagac cagtacaatc ttgcactgtc ccagcaacgg
     2761 gcgcaggcgg tggtggatga gctcacctct gccggcatcg cccgcgaacg aatggcggtg
     2821 aaagcctatg gcgagcaata cccggtggcc agcaacg
//
LOCUS       DASUEG010000388         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2160001, whole genome shotgun sequence.
ACCESSION   DASUEG010000388 DASUEG010000000
VERSION     DASUEG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160001"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="bamC"
                     /locus_tag="VFX11_07150"
     CDS             <1..901
                     /gene="bamC"
                     /locus_tag="VFX11_07150"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX4938434.1"
                     /translation="KDSDDLPRPQALVNVDESAGLELRADEDERQWLVVEQPAATLWP
                     QLRDFLISNGFTIDREDAAKGTLETGWLKPRRHDEEEFGPREGGPDDEEDDGPGFWDS
                     FFSWFSWGEDDERVRFQLSMSAGKDGGDTIVTINQIETSRSSDSLPTADEIEWPDEPE
                     DPELVEILYNELMDFLGEGGRQVGVSVLSQDLKALPKFVMTYDGNGYPVLVINQDFNR
                     AWQEVGMALQRARIPTDDLDRSLGIYYLGKADPDDDEEQPLQLRLSRSETGVQVSVQL
                     DDDALALKEDSARILNRLRESLE"
     gene            901..1731
                     /locus_tag="VFX11_07155"
     CDS             901..1731
                     /locus_tag="VFX11_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4938435.1"
                     /translation="MRFASIGSGSDGNGTLIANGSEGTVLLDCGFSGRETLARLQDRG
                     LSTDDLAGILVTHEHSDHIKGVARLANRYRLPVYTTWGTFSARLNGELDESLLNLVEP
                     GTAFTLAGMEIEPVPVPHDAREPCQFVFATSSARLGILTDAGSINRQMVASYQGCDAL
                     MLECNHDLRMLAEGPYPAQLKRRVAGALGHLNNQQAADMLRQVGHENLQHLIVTHISQ
                     TNNTPALAVRALLDVRSTVEGIIQVAGQKSGLDWCSINRKQNSATPVPPRQLAIDFEI
                     "
     gene            1782..2495
                     /gene="purC"
                     /locus_tag="VFX11_07160"
     CDS             1782..2495
                     /gene="purC"
                     /locus_tag="VFX11_07160"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX4938436.1"
                     /translation="MEKRQELYKGKAKSVFTTDDPNVYVMLYRNDTSAFDGKKVEQLD
                     RKGMVNNKFNAFIMQKLEAAGIETHFIKLLNDTESLVKKLEMIPLECVVRNISAGSIC
                     KRLGVPEGQDLNPPTFEFFLKNDPLGDPMVNEYHIRSFGWATPEQVEKMKELTFKVNG
                     VLKQLFLDGGMLLVDYKLEFGVYEGRVILGDEFSPDGCRLWDKDTRKKLDKDRFRQGL
                     GGVVESYEEVGHRLGVQFD"
     gene            complement(2601..>3950)
                     /locus_tag="VFX11_07165"
     CDS             complement(2601..>3950)
                     /locus_tag="VFX11_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938437.1"
                     /translation="GLETVLTPFFIIYLLMRLAKPEEMLEKTTPLGLVFAFAFLVRLD
                     AIAVVPAYLLFDFILNRRRLQPHYLMRLARVLAPLAVTGLTYAALNQYWFGTPVPVSG
                     LAKALDTGRFNNFGILHTYVMSCLPLFPIRSEWLPLLFWSTVILIELARRCLCGRLQA
                     HAIPLLFLLLCAGIQYLYYACFSGWNIWPWYLYFTPFILFFVIARTANSLLILARHLR
                     VGGKRLAEDSSGFTQTRLHTGMAITLLLAAGLYTATDKFRAGIQTADPARLTYAKLSM
                     QQCGLYRDKTIAFGDRAGSLGYWCPTQIAQTEGLVMSRDFLHARAAGTGEQWLDDHYD
                     IDLYVVDRDRIPVKSGTPDIYIVVDPIQGRTAHKALLHYCFPATALLDSKRYGADASR
                     FIFDYRQKIPCDAAAERIIAAIQAHEGMRRYSLPAEYRNSRLKTWLENIDRRLADLQ"
BASE COUNT          836 a         1053 c         1277 g          784 t
ORIGIN      
        1 caaggacagc gacgatctgc cgaggcccca ggccctggtg aacgtcgatg aatcggccgg
       61 tcttgagctg cgggccgacg aggacgagcg ccagtggctg gtggttgagc agccggcagc
      121 cacactctgg ccgcagttgc gggatttcct gatctccaac ggtttcacca tcgaccgcga
      181 ggatgccgcc aaaggcaccc ttgaaaccgg ctggctgaaa ccccgtcgcc atgacgagga
      241 agaatttggc ccccgcgaag gtggtcccga tgacgaggaa gatgacggcc cgggtttttg
      301 ggattcgttc ttcagctggt tcagctgggg cgaagatgat gagcgggttc gattccagct
      361 gtcgatgtcg gccgggaagg atggcgggga caccatagtg accatcaacc agattgaaac
      421 cagccgcagc agcgattccc tgcccaccgc agacgagatc gaatggccgg atgagccgga
      481 agatcccgaa ctggtggaga ttctctacaa cgaactgatg gatttccttg gcgaaggtgg
      541 ccgtcaggtg ggcgtgtcgg tgctgtcgca ggatctgaag gcgttgccga aattcgtgat
      601 gacctacgac ggcaatggtt atccggtgct ggtgatcaat caggatttca accgcgcctg
      661 gcaggaagtg ggcatggcat tgcagcgggc ccgtattccg accgacgacc ttgatcgcag
      721 tctgggcatt tattatctgg gcaaggccga tccggatgac gacgaggagc aaccgctgca
      781 attgcggctg tcccgttccg agaccggtgt gcaggtgtcg gtgcagctgg atgacgacgc
      841 gctggcgctg aaggaagatt cggcccgcat cctcaaccgt ctgcgggaga gcctggagta
      901 gtgcggttcg cttccattgg cagcggcagc gacggcaacg gcacgctgat cgccaatggc
      961 agcgagggca ctgtactgct ggattgcggc ttttccgggc gggaaaccct tgcccggctg
     1021 caggaccggg gcttgtccac ggacgatctg gccggcattc tggtcaccca tgagcacagc
     1081 gatcatataa agggtgtcgc ccgactggcc aatcgttacc ggctgccggt gtacaccacg
     1141 tggggcactt tcagcgcacg gctcaacggc gagctggatg aaagcctgct caacctggtt
     1201 gaaccgggca ccgcgttcac gctggccggg atggagattg aaccggtgcc ggtgccgcac
     1261 gatgcccgcg aaccctgcca gtttgtgttt gccacttcca gtgcgcgtct gggtattctt
     1321 accgatgccg gttcgatcaa ccggcagatg gtggccagtt accagggttg cgatgcgctg
     1381 atgctggaat gcaaccacga cttgcggatg ctggcggagg ggccttatcc tgcgcagctc
     1441 aagcggcggg tagccggcgc gctggggcat ctgaataacc agcaggcagc tgacatgctg
     1501 cggcaggtgg ggcatgagaa tctgcaacac ctgatcgtga cccacatcag ccagaccaac
     1561 aatacaccgg ccctggcggt acgggcgtta ctggatgtga ggtccacggt ggagggcatc
     1621 atccaggtgg ccggtcagaa gagtgggctg gactggtgca gcatcaatcg caagcagaat
     1681 tccgccacgc cggtgccgcc gcgccagctg gccatcgatt tcgaaatctg aacagcacac
     1741 ttgaatccta accaaccggg tatagcgtaa aggtaataac aatggaaaag cgtcaggagc
     1801 tttacaaggg taaagccaaa tccgttttca ccaccgatga tcccaatgtc tacgtcatgc
     1861 tgtatcgcaa tgacacttcc gcgttcgacg gcaagaaggt cgagcagctg gaccgcaagg
     1921 gcatggtcaa caacaagttc aatgctttca tcatgcagaa gctggaagca gcgggcattg
     1981 aaacgcactt catcaaactg ctgaacgaca ccgagtccct ggtgaagaag ctggagatga
     2041 ttccgctgga gtgcgtggtg cgcaacattt ctgccggcag catctgcaag cgtctgggcg
     2101 tgccggaagg ccaggatctg aatccgccca cgtttgagtt cttcctgaag aacgatcccc
     2161 tcggcgatcc gatggtgaat gaataccata tccgcagctt tggctgggcg acgccggagc
     2221 aggtggagaa aatgaaggaa ctgaccttca aggtgaacgg cgtgctcaag cagctgttcc
     2281 tggatggcgg catgctgctg gtggattaca agctggaatt cggcgtgtat gaaggtcgcg
     2341 tgattctggg cgacgagttc tcgcctgatg gctgccgcct gtgggacaag gacacccgca
     2401 agaagctgga caaggaccga ttccgtcagg gtctgggcgg tgtggtggaa tcctacgagg
     2461 aagtgggaca ccggttgggc gtccagttcg actgattgtt gttttactgg tacttgtttt
     2521 acagctactt gttttacgaa agccctggcg tttgtgccgg ggctttttgt ttgggccgga
     2581 ttcaggggct tgattaaggg ttattggaga tcagcaaggc gccggtcgat attttccagc
     2641 caggttttca gccggctgtt gcgatattcc gcagggagtg agtagcggcg catgccttcg
     2701 tgtgcctgga tggcggcaat gatgcgctct gctgctgcgt cgcagggaat tttttgccgg
     2761 tagtcgaaaa tgaagcggct ggcgtcagcg ccataacgct tggaatccag cagtgcagtc
     2821 gccgggaagc agtagtgcag cagggctttg tgtgccgtgc gtccctggat ggggtccacc
     2881 acgatgtaga tatccggtgt gccgcttttg acgggaatcc ggtcgcgatc cacgacgtag
     2941 agatcgatgt cgtagtgatc gtccagccat tgttcgcctg tgcctgccgc gcgggcgtgc
     3001 aggaaatccc gtgacatcac cagcccttcg gtctgcgcaa tttgcgtggg acaccagtag
     3061 cccaggctgc cggcgcggtc gccgaaggca atggtcttgt cacggtacag gccgcattgc
     3121 tgcatgctca gtttggcgta ggtcaggcga gccggatcag cggtctgtat gccggccctg
     3181 aacttgtcgg ttgcggtgta cagtcccgca gccagcaaca aggtgattgc cattcctgtg
     3241 tgcaggcggg tttgtgtaaa tccgctgcta tcttccgcca accgttttcc gccaacgcgc
     3301 agatgtcgcg ccaggatcaa cagactgttt gccgttctgg cgatgacaaa aaacagaatg
     3361 aaaggcgtga agtacaggta ccagggccag atattccagc cggagaagca ggcgtaataa
     3421 agatactgga tgcccgcgca cagcagcagg aagagcaggg gaatggcgtg agcctgcaag
     3481 cggccgcaca ggcagcgtcg cgccagttcg atcaggatga ccgtgctcca gaacagcagt
     3541 ggcagccatt ccgaacgtat gggaaacaat ggcagacaac tcatcacgta agtgtggagg
     3601 atgccgaaat tattgaagcg gccggtgtcc agtgccttgg cgaggccgct gacgggcacc
     3661 ggcgtgccga accagtactg gttgagcgcg gcataggtca ggccggtcac tgcaagcggc
     3721 gccagaaccc gggccaggcg catcaggtag tgcggctgca gacggcgccg gttgagaatg
     3781 aaatcgaaga gaagataagc cggcacgaca gcaatggcgt cgagccgcac cagaaatgcg
     3841 aacgcaaaca ccaggcccag tggcgtggtt ttttccagca tctcttccgg tttcgccaat
     3901 cgcatcagca ggtaaatgat gaagaacggc gtcagtacgg tttccaggcc
//
LOCUS       DASUEG010000389         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2143842, whole genome shotgun sequence.
ACCESSION   DASUEG010000389 DASUEG010000000
VERSION     DASUEG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143842"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFX11_07170"
     CDS             complement(<1..344)
                     /locus_tag="VFX11_07170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4938438.1"
                     /translation="MELDETLRRQRVEKAAAVYSPLLLSVYDLWVYKVAAPRFFRCPL
                     RIFQHFYEQNVSRHHLDVGVGSGILLRHCRQKNLLDQVALLDLNPNCLDATERALQPL
                     KVRKYCADILQPL"
     gene            complement(424..846)
                     /locus_tag="VFX11_07175"
     CDS             complement(424..846)
                     /locus_tag="VFX11_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX4938439.1"
                     /translation="MNYDNQNIFAKILRGELPCQKVYEDDHSLAFMDIMPQADGHTLV
                     IPKYPAVTLLDIPADHLAALMASTQKVAAAVKKAFAAPGIVLFQLSGAEAGQTVPHLH
                     FHVLPSSLRTLMTRAHAAQMEDQAKLAEFAARIRAALG"
     gene            complement(910..1848)
                     /gene="lpxC"
                     /locus_tag="VFX11_07180"
     CDS             complement(910..1848)
                     /gene="lpxC"
                     /locus_tag="VFX11_07180"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX4938440.1"
                     /translation="MITHSPSQYFQHTLNRSISFVGRGLHTGLAIRMMIQPAPANTGH
                     VFVRQDVPLDQAEVPARWNTVTDTRLSTTVANRFGVKVATIEHLMAALHACGVDNARI
                     VLNGPEVPIMDGSAEPFVRLIRQVGLQQQEEERRAILVRKPVRVEEEGKSASLLPNPR
                     PWMDMTIEFDSEVIGHQHLSLSVEAESFERELANARTFGFEDQINTLRKLGLAKGGSL
                     QNAVLVNENGVVNAEGLRHADEFVRHKILDAIGDLSLAGMPIIGRFNGFCSGHGLNNT
                     LLRKLLQSVDSWTLTTLRGAEEYWNWTRVQKMPSGW"
     gene            2000..>2544
                     /locus_tag="VFX11_07185"
     CDS             2000..>2544
                     /locus_tag="VFX11_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4938441.1"
                     /translation="MASTTLSSWALLVWEELQQRELGAREIFIAAGLDPARLQDPNAR
                     FPVPGMQRLWQLAEERSGDPAFGVAAGMRWNATTFHALGYAWLASATLAQAFHRLARY
                     GRLVNDGTEFSLSAIGLQYRFSGGLKDTMQGSVPVAEQAALVAIVKMCRMLQGESFSP
                     AEVQFTFAPTPSTLALETNLRA"
BASE COUNT          446 a          819 c          794 g          485 t
ORIGIN      
        1 agcggctgca ggatgtcggc gcagtatttg cgcaccttga ggggttgcag ggcccgctcg
       61 gtggcatcca ggcagttggg attcaggtcc agcagggcca cctggtccag caggtttttc
      121 tgccggcagt gccgcagcag gatgccggag ccaaccccga catccagatg gtggcggctc
      181 acgttctgct cgtaaaaatg ctggaagatg cgcagcggac agcggaaaaa gcgtggtgcc
      241 gccaccttgt acacccagag gtcatacacc gacagcagca gcggcgaata gacggcggcc
      301 gccttctcca cccgctgccg gcgcaaggtt tcgtcaagtt ccatagtggt tcccgttggc
      361 ccggggccaa ttcctttatt ttgaatgtgc agtgcgaatg aaaaacggga gcaggcccgc
      421 ggctcagccc agcgccgccc ggatgcgcgc cgcaaactcc gccagcttgg cctgatcttc
      481 catctgcgcg gcgtgggccc gcgtcatcag ggtgcgcaac gaactgggca gcacgtgaaa
      541 atgcaggtgg ggcacggtct ggccggcttc ggcgccgctc agctggaaca gcacgatgcc
      601 cggcgcggca aacgcctttt tcaccgccgc cgccaccttc tgcgtgctgg ccatcagggc
      661 ggccagatga tcggcgggaa tatcgagcag ggtcactgcc gggtacttgg gaatcaccag
      721 ggtgtggccg tcggcctggg gcatgatgtc catgaacgcc aggctgtggt cgtcttcgta
      781 gactttctgg cagggcagtt caccccgcag gattttggcg aaaatgttct gattgtcgta
      841 gttcatgggt tctttccccc cgcatcaccc caaggatgcg ggagaatact gcagcccccc
      901 ctgaaacaac taccaaccgc ttggcatttt ttgaacgcgg gtccaattcc agtattcctc
      961 ggcaccgcgc agggtggtca acgtccagct atcgacactc tgcagcaact tgcgcagcag
     1021 cgtgttgttc aggccgtgcc ccgagcagaa gccgttgaag cggccgatga tcggcatgcc
     1081 cgccagcgac aggtcgccaa tggcatccag gatcttgtgg cggacgaatt cgtcggcatg
     1141 gcgcaggccc tcggcattga ccaccccgtt ctcattcacc agcaccgcgt tctgcagtga
     1201 gccgcccttg gccagaccca gcttgcgcag ggtattgatc tggtcctcaa agccaaaagt
     1261 gcgcgcattt gccagttccc gttcaaagga ttccgcttcc accgatagcg acaggtgctg
     1321 gtggccgatc acctcggaat caaactcgat cgtcatgtcc atccagggac gcgggttcgg
     1381 cagcaggctg gcggatttgc cctcctcctc cacccgcacc ggctttcgca ccaggatcgc
     1441 ccggcgttcc tcctcttgct gctgcaggcc cacctgacgg atcaggcgta cgaaaggttc
     1501 cgcgctgcca tccatgatcg gcacttccgg gccgttcagc acgatgcggg cattgtccac
     1561 accgcaggca tgcagcgctg ccatcaggtg ctcgatggtg gccaccttca cgccaaagcg
     1621 gttggccacg gtggtggaca gccgggtgtc ggtgacggtg ttccagcgcg ccggcacttc
     1681 agcctggtcc agcggcacgt cctggcggac gaagacatgg ccggtgttgg ccggtgccgg
     1741 ctggatcatc atgcggatcg ccagaccggt atgcaggccg cgaccgacaa agctgatgga
     1801 acggttcagc gtgtgctgaa aatactggct gggcgagtgg gtaatcatgc gattctcctg
     1861 tcaggtgttg cgacaggttc agattacgaa ttgcccctta gcggcgctga tcccgccccg
     1921 ccactccctt gtcagcgcct tgtcatttgg cgccaagggt ttatccttcc ccgacaacgg
     1981 cttatccgga cggattttca tggcatccac cacgctctcc tcctgggctc tgctggtgtg
     2041 ggaagaactg cagcagcgcg aactgggcgc gcgggagatt ttcatcgccg ccggcctcga
     2101 cccggcccgg ctgcaggacc ccaacgcgcg cttcccggtg ccgggcatgc agcggctgtg
     2161 gcaactggcg gaggaacgct ccggcgaccc ggcgttcggc gtcgccgccg gcatgcgctg
     2221 gaacgccacc acttttcacg cccttggcta tgcctggctg gccagtgcca ccctggccca
     2281 ggcgtttcac cgtctggccc gctatggccg cctggtcaac gacggcaccg aattcagcct
     2341 cagtgccatc ggcctgcagt accggttcag cggcgggctg aaggacacaa tgcaggggtc
     2401 tgtaccggtg gcggaacagg ctgccctggt ggccatcgtg aagatgtgcc ggatgctgca
     2461 gggggaaagc ttcagcccgg cggaagtgca gttcacgttc gcccccaccc ccagtaccct
     2521 ggcgctcgaa accaacctgc gggc
//
LOCUS       DASUEG010000390         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_332441, whole genome shotgun sequence.
ACCESSION   DASUEG010000390 DASUEG010000000
VERSION     DASUEG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332441"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFX11_07190"
     CDS             <1..1157
                     /locus_tag="VFX11_07190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4938442.1"
                     /translation="DPLGRPLDGLSTPNTTHSRPVESRSPPIMARAYVQRPLHTGNTI
                     VDTLLPVGRGQRQLIIGDAGTGKTAIALDAVINQKPADVRCIYVLIGQRRSAVQEVIT
                     QLRKTGAIANTTVIVAEASALPGLQYLAPYTGCAIAEAWMNDGHDTLIVYDDLGQHAL
                     AYRELSLLFRRPPGREAYPGDIFYLHARLLERATQLAPEQGGGSMTALPIVPTEQGEL
                     ASYIPTNLISITDGQIYLNQNLFASGIRPAIDIGRSVSRIGGKAQDRAIAKAAGRLRL
                     DYLQFLELELFTRFGTKLEAGMAARVARGRQLQILFGQDRLVPRPIHYQMAWLVAWNE
                     GWLTDLAPAELKQRLTQLMAIATTPEALPPLDASHERWRAAVAHWLERTL"
     gene            1154..1945
                     /locus_tag="VFX11_07195"
     CDS             1154..1945
                     /locus_tag="VFX11_07195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX4938443.1"
                     /translation="MSQALLRRRALLLDELHQIVTAMRNMSVAALQRLGKAAEVEQEA
                     LRLLTQALADMNTVLPPVSGAGICLVIGSERGFCGGFNSRIAEFSKRHAAEHPGLHFY
                     AAGSRLVPLLENEWPQLNVLNGCADMEDASSTVSGWLSAVLPSEHNWPPLTLIYHDDG
                     GVKARSLLPLPPLPKPGHGPAPLRYLDAAPLMAGFVSEWLRLSLLNTLTTSLTTEHHW
                     RLNQMQRADRYLEEAGIKLRGRMQRQRQNEITVELENLMSALDVG"
BASE COUNT          414 a          699 c          626 g          382 t
ORIGIN      
        1 tcgatccatt gggccgcccg ctcgacggcc tcagcacgcc gaacaccacc cacagccggc
       61 cagtggaatc gcgttcgcct cccatcatgg cccgcgctta tgtgcagcgg cccctgcaca
      121 ccggcaatac cattgtcgat accttattac cggtcggtcg tggccagcgc cagctgatca
      181 tcggcgatgc cggcactggc aaaaccgcca tcgcactgga tgccgtcatc aaccagaagc
      241 ctgccgatgt gcgttgcatc tacgtgctga ttggccagcg ccgttccgcc gtgcaggaag
      301 tcatcaccca gctgcgcaaa accggcgcca tcgccaacac cacggtgatc gtggcggaag
      361 ccagtgcact gccggggctg caatatctgg cgccctacac cggctgcgcc atcgccgaag
      421 cctggatgaa cgacggccac gacacactga ttgtctacga cgatctcggc cagcatgcgc
      481 tggcatatcg tgaactgtcg ttgttgttcc ggcgtccgcc cggacgcgaa gcctaccctg
      541 gcgacatttt ttatttgcac gcccgcctgc tggaacgcgc tacccaactc gcgccggaac
      601 agggcggtgg cagcatgacg gcattgccca tcgtgcccac ggaacagggc gaactggcca
      661 gctatattcc caccaacctg atatccatca ccgacggcca gatttacctg aaccagaatc
      721 tttttgcgtc cggcatccgc cccgccatcg acatcggccg ctccgtttcc cgtatcggcg
      781 gcaaggcgca ggacagagcc atcgccaaag ccgccggacg tctgcgactg gactatctgc
      841 aatttctcga actcgaactg ttcacccgct tcggcaccaa gctggaagcg ggaatggccg
      901 cacgcgtcgc acgcggccgc caattgcaaa tcctgttcgg ccaggatcgt ctggtgccgc
      961 gccccattca ctaccagatg gcctggctgg tggcgtggaa tgaaggctgg ctgacggatc
     1021 ttgcgccggc agaattgaag caacggctga cgcagctgat ggccattgcc accacgcccg
     1081 aggcactgcc acccctcgac gccagccacg aacgctggcg ggcagctgtc gcccactggc
     1141 tggagcgcac gctgtgagcc aggcactgct gcgccgtcgc gctctgctgc tggacgaact
     1201 gcatcagatc gtcacggcca tgcgcaacat gtcggttgcc gcgctgcagc gtttgggaaa
     1261 agctgccgaa gtggaacagg aagccctgcg tttgctgacg caggcactgg cggacatgaa
     1321 caccgtattg ccaccggtca gcggcgccgg aatctgcctg gtcatcggca gcgaacgtgg
     1381 tttctgcggc ggtttcaaca gccgcatcgc cgaattcagc aaacgtcatg cggctgaaca
     1441 cccaggattg catttttacg cggccgggag ccgtctggtc ccgttgctgg aaaatgaatg
     1501 gccgcaatta aacgtgctga acggctgcgc agatatggaa gacgcgtcca gcaccgtttc
     1561 cggctggctc tccgcagtgc tgccgtctga acacaactgg ccaccgctga cactgattta
     1621 tcacgacgac gggggcgtca aggcccgatc gctattgcca ttgccacccc tgcccaaacc
     1681 aggccacggc cccgcaccct tgcgttacct ggacgctgcg ccgttgatgg cgggttttgt
     1741 cagcgaatgg ctgcgcctga gcctgctgaa caccctcacc acctcgttga caacggaaca
     1801 ccactggcga ctgaaccaga tgcagcgcgc ggatcgttat ctggaagaag cgggaatcaa
     1861 gttgcgcggc aggatgcaga ggcaacggca aaacgagatc acggttgagc tggaaaacct
     1921 gatgagtgcg ctggatgttg ggtgaggggg atttcggaag tgagtaatgc agggtgagat
     1981 agaacgcgca ggagggatta ctcggcgctg cgcgcctcgc cccttcgggg ccgtcggccg
     2041 atggccgacg ttcctccgcc gcttgcagcg gctccggtcg aacccgctgc ggcttctggc
     2101 ccctccctca ccgccatata a
//
LOCUS       DASUEG010000391         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1891735, whole genome shotgun sequence.
ACCESSION   DASUEG010000391 DASUEG010000000
VERSION     DASUEG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891735"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFX11_07200"
     CDS             complement(<1..402)
                     /locus_tag="VFX11_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX4938444.1"
                     /translation="MAPNKKAHPLQADIDALLRAEHGDPFSFLGMHSNADGKIVVRAL
                     QLSAEKVEVIDADSGKVLAELKCVDAAGVFEGTIPRRKERFPYRLLVTWNGVSRELED
                     PYRFPSLMGETDAWLFGEGNHLRPFEFLGAHP"
     gene            complement(544..1986)
                     /gene="glgA"
                     /locus_tag="VFX11_07205"
     CDS             complement(544..1986)
                     /gene="glgA"
                     /locus_tag="VFX11_07205"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX4938445.1"
                     /translation="MKVLYVTAELFPLVKTGGLADVSAALPPVLRWLGLDVRLCLPCY
                     PVFREAIPDLVEVMHLGSAFGCRQVRILRGITPQTDVPIYLIDAPELFDRPGNPYFGP
                     DGHLWHDNHRRFALFGWAAAQLRDLDTGWSPDILHAHDWHAGLACAWLRARPRGRVRS
                     LFTIHNLAYQGNFTGDIFPELGLPQEFFDMYGLEFYGKVSFMKAGLYYADWLSTVSPN
                     YAREIQTSEHGYGMEGLLHQRRDRLVGILNGVDYDVWDPSDDPTLPHTYNQKNAESKQ
                     LNKAVLQAALDLEMNPTAPLFCVISRLTSIKGLDMVVEVTDAIVKQGGQLVVIGQGDN
                     ELEYKFRELATLYPQSVATYIGYQENISHLVLASSDCLIMPSRSEPCGLTQLYAMKYG
                     TIPLVCRVGGLADTVVDATPEKLMRGDATGFVFRENHLPSLMDAIDRAFEMYRNGRAW
                     TRLQRSAMSQNFSWQQSGRRYLELYNRMRA"
     gene            complement(1992..>2164)
                     /gene="glgC"
                     /locus_tag="VFX11_07210"
     CDS             complement(1992..>2164)
                     /gene="glgC"
                     /locus_tag="VFX11_07210"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246607.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX4938446.1"
                     /translation="GQFARLKKVLVDRGCRIPSGLVVGEDPNEDARRFYRSEGGVTVI
                     TREMLERLGYPS"
BASE COUNT          470 a          664 c          635 g          395 t
ORIGIN      
        1 cgggtgggcg ccgagaaact cgaagggacg cagatgattg ccctcgccaa acagccaggc
       61 atccgtctca cccatcaggg aagggaaacg ataagggtct tccagctcac gcgacacgcc
      121 attccaggtt accagcaggc gataggggaa gcgttccttg cgtcgcggaa tggtcccttc
      181 gaatacgcca gcagcgtcta cacacttgag ctcagccagc actttgccgc tatccgcgtc
      241 aatcacctca actttttcgg cggaaagctg cagggcacgc accacgatct tgccgtcggc
      301 gttggagtgc atacctaaaa agctgaacgg atcgccgtgt tcggcacgca gcagggcgtc
      361 aatgtccgcc tgcagcggat gcgctttttt attgggggcc atcaaatgtc atcctcagtt
      421 tttctcaaaa acaccactga acggcggaaa cgtcggtctg ttacaacagt gcacgcgctc
      481 gccggccaat tcatccatca atatagttat caaacagcgc ggtcgtaata ctacctgagg
      541 ccattacgcg cgcatacggt tatacagctc cagatagcgg cgaccggact gctgccagct
      601 gaaattctgg ctcatggctg aacgctgcag acgcgtccag gcccggccgt tgcgatacat
      661 ctcgaatgcg cggtcgatgg catccatcag cgacggcaga tgattctccc tgaaaacaaa
      721 tccggtagca tccccacgca tgagtttttc cggtgtggca tccaccaccg tatcggccag
      781 accgcccacg cggcacacca gcggaatggt gccgtacttc atggcataca gctgtgtcag
      841 gccgcatggt tcggagcgcg acggcatgat caggcagtcg ctggaggcca gtaccagatg
      901 gctgatgttt tcctgatagc cgatataggt agccacggac tgcggataca aggtggccag
      961 ttcgcggaat ttgtattcca gctcattatc gccctgaccg atcaccacca gctggccgcc
     1021 ctgcttgacg atggcgtcgg tcacctcgac caccatgtcc agccccttga tgctggtcag
     1081 gcggctgatg acgcagaaca gcggagccgt gggattcatc tccagatcca gcgcagcctg
     1141 cagcacggcc ttgttgagct gcttggattc ggcgtttttc tggttatacg tgtgcggcag
     1201 ggtcgggtca tccgacggat cccacacgtc gtaatccacg ccattgagaa tgcccaccag
     1261 ccggtcgcgc cgctggtgca gcaatccttc catgccgtaa ccgtgttcgc tggtctggat
     1321 ttcccgggcg taattggggc ttacggtgga gagccagtcc gcgtaataca gccccgcctt
     1381 catgaacgac accttgccgt agaactccag gccgtacata tcgaagaatt cttgcggcag
     1441 gcccagctcg ggaaagatgt cgccggtgaa attaccctgg taggccagat tgtgaatggt
     1501 aaacagcgag cgcacgcggc cgcgcggacg ggcgcgcagc caggcacagg ccaggccggc
     1561 gtgccagtca tgggcgtgca ggatgtccgg cgaccagccg gtatccagat cgcgcaactg
     1621 ggcggcggcc cagccaaaca gggcaaaacg gcggtgattg tcatgccaca ggtggccatc
     1681 cgggccgaaa tacggattgc cggggcggtc gaacagttcg ggggcatcaa tcagatagat
     1741 cggcacatcg gtctggggcg tgatgccgcg caggatgcgc acctggcgac agccaaaggc
     1801 cgagcccaga tgcatgactt ccaccagatc aggaatggcc tcgcgaaaca ccgggtaaca
     1861 cggcaggcac agacgcacgt ccagccccag ccagcgcaat accgggggca gggccgcgga
     1921 cacatcggcc agacccccgg tcttgaccag aggaaacagt tcggcggtaa cgtacagaac
     1981 cttcagcatg ttcaggaggg gtagcccagt cgctccagca tttcacgggt aatcaccgtg
     2041 accccgccct ccgaacgata gaaacggcgc gcgtcttcgt tcggatcttc gcccaccacc
     2101 agaccggagg gaatgcggca gccgcgatcg accagcacct tcttcaggcg cgcgaactga
     2161 ccta
//
LOCUS       DASUEG010000392         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1423562, whole genome shotgun sequence.
ACCESSION   DASUEG010000392 DASUEG010000000
VERSION     DASUEG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423562"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFX11_07215"
     CDS             <1..425
                     /locus_tag="VFX11_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209070.1"
                     /note="5'-3' single-stranded-DNA-specific exonuclease;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX4938447.1"
                     /translation="PVEKYESFQQAFDKEARRLLTLDDLTGVLHSDGELSQSDIDLPL
                     ARMLREAGPWGQGFPEPLFDGEFELLDQRIVGEKHLKLTLGFPASKRVIDAIAFNVDL
                     EKWPNKKARRIYLAYKLDVNEFRGVQNPQLMVEHLEAV"
     gene            complement(481..1281)
                     /locus_tag="VFX11_07220"
     CDS             complement(481..1281)
                     /locus_tag="VFX11_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX4938448.1"
                     /translation="MKILFALGWMLLVSVSLSLLLLPTCVALTLLQPDTLLSGIGLAL
                     LIPPALLAGFFLVLGLTWLYCRALPPTPQGKFNMFEDRGAVLWALNNALPTLLLKFFQ
                     STLFLSEHLRYCLLRACNADVRFTSWIPTNASIADLRNLHLGENVVIGEHCTLGPAFQ
                     PRPYRLVVSDIHIGDNSLVGAHSMVAAGVHIGANVLIQAQVMIASGSRLGNNVKIGVG
                     TRIDTYCHIGEGAVVGKWVTLLSRAQIPDGAQVPDGAVVKGKWIDPQQ"
     gene            complement(1278..>2200)
                     /locus_tag="VFX11_07225"
     CDS             complement(1278..>2200)
                     /locus_tag="VFX11_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX4938449.1"
                     /translation="TLPRQSELARQVSNWQRAGNDPVALTALLFPHLKYFACWLGGNM
                     GHARDNLFRIMGEKSVFEMPFSASEGVFAVPHKLNEPGGIAAVTSHFLEYIPEDAIDD
                     PAAPTLCAWELETGGTYYQVVSTQAGLYRYNMEDLIRVKDHWGGIPVVEFISKKARQI
                     SIANERLNENDVTEACRVACAQTGALLKQFILFPSRDGFYEMLVEGRIDDPQRFADAM
                     EQALCNVAKGYDFEREDRLLQPLRLLQAQDGQLSDYVHARQFRSALPSAQFKPIHLSN
                     EFEGARNFAIQSRHVAADRYAGGLASQVSA"
BASE COUNT          446 a          624 c          682 g          448 t
ORIGIN      
        1 tgccggtgga aaaatatgaa tccttccagc aggcgttcga caaggaagcg cggcgtttgc
       61 tgacgctgga tgatttgacc ggcgttctgc acagcgatgg cgagttgtcg caaagcgata
      121 tcgatctgcc actggcgaga atgctgcggg aagcgggtcc ctgggggcag ggttttcccg
      181 aaccgctatt tgacggtgaa tttgaattgc tggatcagcg catcgttggt gaaaagcatc
      241 tgaaactgac gctgggtttt cccgccagca aacgggtgat cgacgcgatt gcgttcaacg
      301 tcgatctgga aaaatggccc aacaagaagg cgcggcggat ttatctggcg tacaagctgg
      361 acgtgaatga atttcgcggt gtgcagaatc cgcaattgat ggtggagcac ctggaggcgg
      421 tgtgagacgg cgttgggtca gccgttttca cattcgcagt catgcatgtt tcgtttcagc
      481 ttactgttgc ggatcaatcc atttgccctt caccactgcg ccgtccggca cctgcgcacc
      541 atccggtatc tgcgcccgcg acagcagtgt cacccacttg cccaccaccg cgccttcgcc
      601 gatgtggcag taggtatcga ttcgggtgcc cacgccgatc ttcacgttgt tacccagccg
      661 gctgccgctg gcgatcatta cctgggcctg gatcagcaca tttgcaccga tatgcacacc
      721 ggccgccacc atgctgtggg caccaaccag gctgttgtcg ccgatgtgga tatcgctgac
      781 caccagccgg taggggcggg gctggaacgc cggacccagg gtgcagtgct cgccaatgac
      841 cacgttttcg cccaggtgca ggttgcgcag atcggctatg gacgcattgg tgggaatcca
      901 cgaagtaaac cgcacgtcgg cgttgcaggc gcgcagcagg cagtagcgca ggtgttcgct
      961 gagaaacagt gtcgactgga aaaatttcag cagcagtgtg ggcagtgcgt tgttcagtgc
     1021 ccacaacacg gcgccgcgat cctcgaacat attgaatttg ccttgcggtg tgggtggcag
     1081 cgcacggcag tacagccagg tcagccccag caccagaaag aatccggcca gcagggcggg
     1141 cggaatcaac agagccagcc caattcccga cagcagcgta tccggctgca gcaacgtcag
     1201 cgccacgcag gtgggcagca acaacaggct caggctcacc gacaccagca gcatccagcc
     1261 cagcgcgaac agtattttca tgcggacacc tgcgatgcaa gaccaccggc atagcggtcg
     1321 gcagcgacat ggcgggactg gatcgcaaaa ttccgcgcac cttcgaattc gtttgaaaga
     1381 tgaatgggtt tgaactgggc actgggcagg gctgagcgga attgccttgc gtgcacgtaa
     1441 tcactcagtt gcccgtcctg cgcctgcagc agccgcagcg gttgcagcag acgatcctcg
     1501 cgctcgaaat cgtaaccttt cgccacgttg cacagggcct gttccatggc atcggcgaat
     1561 cgctgcggat cgtcgatgcg gccttccacc agcatttcgt aaaatccgtc gcgggatgga
     1621 aacagtatga attgcttcag cagcgcaccg gtttgcgcgc aggcaacacg gcaggcttcg
     1681 gtcacatcgt tttcattcag gcgttcgttg gcgatggaaa tctgccgcgc ttttttcgaa
     1741 atgaattcca ccacggggat gccgccccag tggtctttca cccggatcag gtcttccatg
     1801 ttgtagcggt agaggccggc ctgggtgctt accacctgat agtaggtgcc tcccgtttcc
     1861 agttcccacg cgcacagggt gggcgcggcg ggatcgtcga tggcgtcttc gggaatgtat
     1921 tccaggaaat ggctggtgac agcggcgatg ccaccgggtt cgttgagttt gtgcggtacc
     1981 gcgaacacgc cctcgctggc agagaacggc atttcgaaca cggatttttc acccatgatg
     2041 cggaacaggt tgtcgcgggc gtggcccatg ttgccgccga gccagcaggc gaaatatttc
     2101 agatggggaa acagcagggc cgtgagcgcg accggatcgt tgcccgcccg ttgccagttg
     2161 cttacctggc gggccagttc ggactggcgg ggcaaggtca
//
LOCUS       DASUEG010000393         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1966460, whole genome shotgun sequence.
ACCESSION   DASUEG010000393 DASUEG010000000
VERSION     DASUEG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966460"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2771)
                     /gene="aceE"
                     /locus_tag="VFX11_07230"
     CDS             complement(111..2771)
                     /gene="aceE"
                     /locus_tag="VFX11_07230"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX4938450.1"
                     /translation="MTRERFYDDVDPVETQEWIDALDSVLESVGPERATYLLDRLTER
                     ARIAGVQQTRLTTPYLNTIPVEKEATFRGDLYLERRIRSLIRWNALAMVQRANLTDDE
                     LGGHISSFASSATLYDVGFNHFFRAPTETFGGDLLFYQGHSAPGIYARAFLEGRLSEK
                     QLENFRREVKKEGLSSYPHPWLMPNFWQFPTVSMGLGPIQAIYQAHFMKYLQNRELIK
                     REDRKVWAFLGDGECDEPESLGCISLAGREKLDNLVFVINCNLQRLDGPVRGNGKIIQ
                     ELEGVFRGAGWNVIKVVWGRKWDPLLARDKLGVLRKRMEEVVDGEYQAFKNHGGAFTR
                     EHFFGKYPELRELVADMTDEEIFQLNRGGHDPYKLFAAYHAATHHKGQPTVILAKTVK
                     GYGTGAGESANKTHQMKTLDIDSLKSFRDRFDMPFSDDELAKLPFYRPADDSTEMRYM
                     RERRMALGGYLPSRRTECETLEAPELGVFGPMLEGSKDREMSTTMALVRMMNSLIKLQ
                     NIGERIVPIVPDEARTFGMEGMFRQLGIYSAVGQLYKPADSEQVMYYREDIKGRILEE
                     GINEAGAMAAWIAAATSYSVNNYPMIPFYIFYSMFGFQRIGDLAWAAGDAQARGFLIG
                     ATAGRTTLNGEGLQHQDGHSHILAATIPNCVAYDPAYSYELAVILQDGLRRMYQNKEN
                     VFYYITVQNENYVHPALPKGAEEGILKGMYLLKEGDEANKLRVQLLGSGSILREVEAA
                     AEILADVYGVAADVWSVTSFNELRREAMELQRWNMLHPLEEPKLPYVTRVLRSRKSPV
                     VAASDYMKVHADQIAPYVRNHFTALGTDGYGRSDSREQLRHFFEVDRYFITVAALHAL
                     ADKGLIGRSKVNDAIRRFNINPDKPYPPAA"
     gene            complement(2928..>3201)
                     /locus_tag="VFX11_07235"
     CDS             complement(2928..>3201)
                     /locus_tag="VFX11_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938451.1"
                     /translation="PARAVAMTIAALCFNGKAFAVAVRRFRATDREAEIVLKLAVKMG
                     LDFKDLPTDEDELASEGIRRIHSAQDLRSNVLIFWIVVIAILTIIS"
BASE COUNT          650 a          993 c          891 g          667 t
ORIGIN      
        1 tgctgacccg atcacccggc ttcaccgaaa tttcgatgac atccaccgct gcggttccac
       61 ccagatcagg cactaacacg tctttgactg ccacagtcat atctccttcc ttacgcggcc
      121 ggcgggtagg gtttgtcggg attgatgttg aatcggcgga tcgcatcatt gaccttgctg
      181 cggccgatca agcccttgtc cgccagcgca tgaagcgcgg ccactgtaat gaagtagcgg
      241 tccacttcga agaaatgccg caactgttcg cgggaatcgc tacggccata gccgtcggta
      301 cccagcgcgg taaagtgatt gcgcacgtaa ggcgcgatct ggtcggcatg caccttcatg
      361 taatccgacg ccgccaccac cggactcttg cgactgcgca gcacgcgcgt cacatacggc
      421 agcttgggtt cctccagcgg atgcagcatg ttccagcgct gcagctccat cgcttcgcgc
      481 cgcagttcat tgaaactggt cacgctccac acatccgccg ccacgccgta cacgtcggca
      541 agaatttccg ccgccgcttc cacctcacgc aggatagaac cgctgcccag caactgcacc
      601 cgcagcttgt tggcttcgtc gccttccttc agcaggtaca tgcctttcag gatgccctct
      661 tccgcaccct tcggcagtgc cgggtgcacg tagttttcgt tctgcaccgt gatgtagtaa
      721 aaaacgtttt ccttgttctg gtacatccgg cgcaaaccgt cttgcaggat caccgccagt
      781 tcatacgaat aggcaggatc ataggcaacg cagttcggaa tggtggccgc cagaatatga
      841 ctatggccat cctggtgctg cagaccttcg ccgttcagcg tggtgcggcc cgcagtggca
      901 ccgatcagga aaccgcgcgc ctgggcatcg cctgccgccc aggccagatc gccaatgcgc
      961 tggaagccga acatggagta aaaaatgtag aacggaatca tcgggtagtt attgacggaa
     1021 taggacgtcg ccgcagcaat ccaggccgcc atcgcaccgg cttcgttgat gccctcttcc
     1081 agaatgcggc ccttgatgtc ctcgcggtaa tacatcacct gttccgaatc cgccggcttg
     1141 tacaactggc ccacggcgga ataaattccc aactgacgga acatgccttc cataccaaag
     1201 gtgcgcgcct catcgggcac gatcggcacg atgcgctcac cgatattctg cagcttgatc
     1261 agcgagttca tcatccgcac cagcgccatg gtggtggaca tctcgcggtc cttgctgcct
     1321 tccagcatgg ggccgaacac acccagctcc ggcgcctcca gcgtttcaca ttcggtacgg
     1381 cgcgacggca ggtagccacc cagcgccatc cggcgttcgc gcatgtagcg catttcggtg
     1441 ctgtcatcag cgggacggta gaacggcaac ttcgccagtt catcgtcgct gaacggcatg
     1501 tcgaagcggt cgcggaagga tttcaggctg tcgatatcca gcgttttcat ctggtgtgtc
     1561 ttgttggcag actcgccggc accggtaccg taacctttca cggttttcgc cagaatcacg
     1621 gtgggctggc ccttgtgatg ggtcgctgca tgatacgcgg caaacagctt gtaaggatcg
     1681 tgaccgccac gattgagctg gaaaatttcc tcgtcggtca tgtcggcgac cagctcacgc
     1741 agttccggat acttgccaaa gaaatgctcg cgggtgaagg cgccgccgtg gttcttgaac
     1801 gcctggtatt cgccgtccac cacttcttcc atgcgcttgc gcagcacgcc gagtttgtcc
     1861 cgtgccagta gcgggtccca cttgcggccc cacaccactt taatcacgtt ccagccggcg
     1921 ccgcggaaca cgccttccag ttcctggatg atcttgccgt taccgcgaac cgggccatcc
     1981 agacgctgca ggttgcagtt gatcacaaac accaggttgt cgagtttttc gcgacccgcc
     2041 agcgaaatac agccgagaga ttccggctcg tcacattcgc catcccccag aaacgcccac
     2101 accttgcggt cttcccgctt gatcagctcg cggttctgca ggtacttcat gaaatgggcc
     2161 tgatagatcg cctggatcgg acccagcccc atcgacacgg tggggaattg ccagaaattc
     2221 ggcatcagcc agggatgcgg ataggacgac agcccttcct tcttcacctc gcggcggaaa
     2281 ttctccagct gcttctccga gaggcgacct tccagaaacg cacgggcgta aataccggga
     2341 gcagagtggc cctgatagaa cagcagatcg ccaccgaaag tctcggtggg cgcccggaag
     2401 aaatggttga agcccacatc gtacagggtg gcagacgagg caaaactgga aatgtggcca
     2461 cccagctcat cgtcggtcag gttggcgcgc tgcaccatgg ccagcgcatt ccagcggatc
     2521 agcgagcgga tgcgccgctc cagatacaga tcaccgcgga aagtggcttc cttctcaaca
     2581 ggaatggtgt tcaggtaggg tgtggtgagg cgggtttgct gcacgccagc aatgcgggcg
     2641 cgttcggtaa gacggtccag caggtaggtc gcccgctcgg gccctacgct ttccagcacc
     2701 gagtccagcg cgtcgatcca ttcctgggtt tccaccgggt cgacatcatc gtaaaaacgt
     2761 tcacgggtca ttcaatacaa ctcctggtat tgcaaaactt tttgagtttt gggtttcgct
     2821 tgtggctgta ccgcgcccag ccgtccctac tgtctggtta cagtatagga aggcgtcacc
     2881 gatttggtgc aacgccgccg ggcaacggtg atgaatgctc tacctgatca gctgatgatg
     2941 gtgaggatgg caatcacgac gatccagaag atcagaacgt tactgcgcag atcctgggcg
     3001 gaatgaatcc ggcggatacc ttccgacgcc agttcatcct cgtccgtggg cagatccttg
     3061 aaatccagcc ccatcttcac cgccagtttg agcacgattt ccgcttcgcg gtcggtagcg
     3121 cgaaagcgtc gaacggcaac cgcaaatgcc ttgccgttaa agcagagtgc cgcaatggtc
     3181 atggccaccg cccgcgcggg g
//
LOCUS       DASUEG010000394         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1990712, whole genome shotgun sequence.
ACCESSION   DASUEG010000394 DASUEG010000000
VERSION     DASUEG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990712"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..959
                     /locus_tag="VFX11_07240"
     CDS             72..959
                     /locus_tag="VFX11_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4938452.1"
                     /translation="MNPLVEPFFDPDTFTISYVVHGDGHPACAIIDPVLDYDARAGRV
                     ATHSAQRILDFVASRKLRVEWILETHAHADHLSAAQWLKRRLGGQVGIGEHIREVQQH
                     FARLFGVEGEVPADGSQFDCLLRDGDRLALGDLQIEVLHTPGHTNACVSFRVGNAVFV
                     GDTLFMPDYGTARTDFPGGDAATLYRSIRRILALPADTRLFLCHDYRPGGRPPAWEST
                     VAEQRAHNLLVHDGVTETAFVADRKERDSRLSAPALILPALQVNIRGGHMPEPATNGT
                     RYLKIPLNRIGGASDEGLV"
     gene            complement(1025..1111)
                     /locus_tag="VFX11_07245"
     tRNA            complement(1025..1111)
                     /locus_tag="VFX11_07245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1075..1077),aa:Leu,seq:cag)
     gene            1304..>2606
                     /locus_tag="VFX11_07250"
     CDS             1304..>2606
                     /locus_tag="VFX11_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016501875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX4938453.1"
                     /translation="MTNWSWSDDPNLDSEAKKYENPIPSREYILELLEDRGRPLTHRQ
                     ICAELGVHEEEPVEAVRRRLKAMERDGQLMLNRRGAYGVTNKMDLITGVIIGHRDGFG
                     FLKPDDGSDDLFLSARQMRCGFDGDRVLVRQSGIDHRGRREATVVEVLERNTLQVVGR
                     FFRDNDIGFVAAENRRINQDILIPPGADLGARSGQFVMVEITEQPTYRFQAQGRVIEI
                     IGEHMAPGMEIDVAIRNYDIPHVWPDDVLAAAKKFTGELVKKDYAHRFDLRQTPLVTI
                     DGEDAKDFDDAVFCEKKKGGGWRLLVAIADVSHYVHPSDALDVEAQRRGNSVYFPEHV
                     VPMLPESLSNGLCSLNPQVDRLALVCEMHVSKAGNVTRFSFMEAVICSHARFTYTKVG
                     KILQEADSEEGQALRQQYAPLVPHLENLYDLYHALHEQRNER"
BASE COUNT          527 a          772 c          796 g          511 t
ORIGIN      
        1 atcccgtttg cgcaacggtc tttgtgtgag gccattcaga cgacctgatt cctttcgatg
       61 aggtgcctgc agtgaatccc ctggttgaac cctttttcga tcccgacacc tttaccatca
      121 gttatgttgt gcatggcgac ggccatccgg cctgcgccat cattgacccg gtgctggact
      181 acgatgcccg cgccgggcgg gtggccaccc attccgcgca gaggattctg gatttcgtag
      241 cgtcgcggaa gctgcgggtg gaatggattt tggaaaccca tgcccatgcg gaccacctgt
      301 ctgccgcgca atggctcaag cggcgactgg gtgggcaggt gggcattggt gagcacattc
      361 gggaggtgca gcagcatttt gcccggctgt ttggtgttga gggtgaggtg ccggccgacg
      421 gtagccagtt tgattgcctg ttgcgggacg gtgacaggct cgccctgggg gatctgcaga
      481 ttgaggttct gcatactccc ggccacacga atgcgtgtgt cagtttccgg gtaggtaatg
      541 cggtgttcgt aggggatacc ctgttcatgc cggattacgg caccgcgcgg acggattttc
      601 ccggtggcga cgcggctacc ctctaccgct ccatccgtcg catcctggcg ctgccggcgg
      661 acacccgcct gttcctctgc cacgattatc ggccgggtgg tcgcccgcct gcctgggaaa
      721 gcacggtggc cgagcagcgt gcccataacc tgctggtcca tgacggggtg acggagacag
      781 cattcgtggc ggaccgcaaa gagcgcgaca gccggctgtc ggcaccagca ctgatcctgc
      841 cggcgttgca ggtgaacatt cgcggtggcc atatgccgga gccggccacc aacggcactc
      901 gctacctgaa gattccgctc aaccggattg gcggggcgag cgacgaaggg ctggtatagg
      961 cgggaaaggc ggttcgtgca ggcaataaaa aacccggaac cttgcggatc cgggtttttt
     1021 aagatggtgc cgaggaaagg acttgaacct tcacggccgt aaagccacta acacctgaag
     1081 ctagcgtgtc taccaatttc accacctcgg ctgagggcgc aaaatttaac ggtttgcggt
     1141 gtgcctgtca aacccctgcc gcaaaaaaag ttcaaccagc ccctgcgctg gcattcgctg
     1201 tctacactga cagggtgccg aacaaaagca aaccttcact tgtaaatcac accccaactg
     1261 cctagagtgc cccaaataca cgaatttcac agggatacag tgcatgacaa actggtcctg
     1321 gtcggatgat ccgaacctcg atagcgaagc aaaaaaatac gaaaacccca tccccagccg
     1381 cgaatacatc ctggaattgc tggaagaccg tggccgcccg ctgacccacc gccagatctg
     1441 tgccgaactc ggtgtccatg aggaagagcc ggtcgaagcc gtgcgccgcc gcctgaaggc
     1501 aatggagcgc gacgggcagc tgatgctgaa ccggcgtggc gcctatggcg tcaccaacaa
     1561 gatggatctg attaccggtg tgatcatcgg tcatcgcgat ggtttcggtt tcctcaagcc
     1621 cgatgatggc agcgacgacc tgttcctcag cgcacgccag atgcgttgcg gcttcgacgg
     1681 cgaccgtgtg ctggtgcgcc agagtggcat cgaccatcgc ggccgtcgcg aagccactgt
     1741 cgtcgaggtg ctggaacgca ataccctcca ggtggtggga cgcttcttcc gtgacaacga
     1801 tattggtttc gtcgctgcgg aaaaccgtcg catcaaccag gacatcctga ttccccctgg
     1861 tgccgacctg ggcgccagga gcggccagtt cgtgatggtg gaaatcaccg agcagcccac
     1921 ctaccgcttc caggcccagg gccgggtgat tgaaatcatc ggcgagcaca tggcgccggg
     1981 catggaaatc gacgttgcca tccgcaacta cgacattccc catgtgtggc ccgacgatgt
     2041 gctggccgcc gccaagaaat tcaccggcga gctggtgaaa aaagattacg cccaccgctt
     2101 cgacctgcgc cagacaccgc tggttaccat cgacggtgag gacgccaagg atttcgacga
     2161 cgccgttttc tgcgaaaaga aaaaaggtgg cggatggcgc ctgctggtgg cgattgccga
     2221 cgtgtcccat tacgtgcatc ccagcgatgc gctggacgtg gaagcacagc ggcgcggcaa
     2281 ctcggtgtat ttccccgagc atgtggtgcc gatgctgccg gagtcgctgt cgaacggtct
     2341 gtgctcgctg aatccccagg tggatcgtct tgcgctggtg tgcgagatgc acgtcagcaa
     2401 ggccggcaat gtcacccgct tcagcttcat ggaagcggtg atctgttccc acgcccgctt
     2461 cacctacacc aaggtcggca agatcctgca ggaagcggat tccgaggaag gccaggccct
     2521 gcgccagcaa tatgcaccgc tggtgccgca cctggagaat ctctacgatc tttatcatgc
     2581 gttgcatgag cagcgaaacg agcgcg
//
LOCUS       DASUEG010000395         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1972505, whole genome shotgun sequence.
ACCESSION   DASUEG010000395 DASUEG010000000
VERSION     DASUEG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972505"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..780)
                     /locus_tag="VFX11_07255"
     CDS             complement(49..780)
                     /locus_tag="VFX11_07255"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX4938454.1"
                     /translation="MSLIAIVGGTGLTELEGLQITESLAVTTRYGAPSAPLEFGRFGQ
                     RDVVFLARHGKGHRLPPHQVNYRANIAALRDAGVTDIIAVNAVGGINSAMGAEALVIP
                     HQIVDYTWGREFTFADEGNVIHVDFSFPYTESLRHGLLAAADKTGHFVHNRGVYAATQ
                     GPRLETAAEVDRLERDGCDIVGMTGMPEASLAREAGLNYACLALVVNMAAGRSDGIIT
                     MAEIELALKNGMGRVRSLLAQFLST"
     gene            complement(944..1504)
                     /locus_tag="VFX11_07260"
     CDS             complement(944..1504)
                     /locus_tag="VFX11_07260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX4938455.1"
                     /translation="MSVSVAEVQTVWKEADCLWTAQQVQASITRIAQTISADLGDKNP
                     LVLVVMKGGMVFGAQLLMQLQFPLELEYVHVSRYGSGLSGGADMLWKVAPPPSLAKRH
                     VLVVDDILDEGVTLSAIMQACESQGARSVKSVVLVDKQHDRKADADFRADYTCLEIPD
                     RYIFGYGMDYKGYLRNADGIFAVKGQ"
     gene            complement(1577..2599)
                     /gene="nagZ"
                     /locus_tag="VFX11_07265"
     CDS             complement(1577..2599)
                     /gene="nagZ"
                     /locus_tag="VFX11_07265"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX4938456.1"
                     /translation="MPLGPLMLDLEGTALLDYEAQRLQHPAVGGVILFARNIVQPEQV
                     RELIASIRAVRPDLILAVDQEGGRVQRVKQGVTKLPPLAQLGERFDADPKAAIASARE
                     WGWLMASEMLALGFDISFAPVLDLNVGRSSVIGTRAFHGDPAIVVVLAEAYIDGMHEA
                     GMAATGKHFPGHGWVEADSHHAIPVDERSWSDIAACDLQTFSQLAGQLDAVMPAHVIY
                     PQVDPNPAGFSRHWLQTVLRTELQFDGVIFSDDLTMEGASVAGDYGQRAQAALNAGCD
                     MVLVCNNPAGAQAVIEYLEQHPAVVNQARLQRMRARRRPDWSQLLADAHYHAVQADMA
                     GQGVAG"
     gene            complement(2650..3144)
                     /locus_tag="VFX11_07270"
     CDS             complement(2650..3144)
                     /locus_tag="VFX11_07270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX4938457.1"
                     /translation="MSRLEIHVSIDQQILELREQGSEKLLARWPVSTALKGAGNAEGS
                     NQTPLGRHRVRARIGAGQPIYTVFRGRRPTGEIYSDAIGREFPHRDWILTRILWLCGE
                     EKGFNRGGCVDSQRRYIYIHGTPDTEPMGEPRSHGCIRMRNQDVLDLFERVPVGTPVI
                     ITQA"
     gene            complement(3137..3772)
                     /locus_tag="VFX11_07275"
     CDS             complement(3137..3772)
                     /locus_tag="VFX11_07275"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938458.1"
                     /translation="MAQKETVERILDAAEVLFAERGFAETSLRTITSAAGVNLAAVNY
                     HFGSKKSLIQAVFVRFLDPLVDEIERQLNQLQAEKERLSVEDLLQVLTSSVYEIHGRG
                     SNRATSFMRLLGLAYTQSQAHLRKFIGERYGSAFQHYASHLKPALPGATAADLFWHTH
                     FALGTAIFTMSNFDALRAMSENDTGERTPVDFIVSRLTAFMAAGLHNAAHE"
     gene            complement(3841..>4740)
                     /locus_tag="VFX11_07280"
     CDS             complement(3841..>4740)
                     /locus_tag="VFX11_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938459.1"
                     /translation="RTVFWGVVEFNHLVDIINQVDLAERPDGEAYLGQPMVNLSLVQD
                     WGIVDLYLLAGFRERIFPGEDGRFRLPLEVAEDAAEYESGEEDQHLDVALRWTRSVDA
                     WELAASLFAGTGRDPEFNSLTPDFKLVPYYPQIAQAAVEVQYTHEFWLWKLEALTRSG
                     EEGGRYAATAFGFEYTLEGWLDTAADLGLVAEYNWDERGAEAPTYLNDDVALGTRLAL
                     NNEADTDFLLGVVYDPATRERLWLIEGHHRIGDAWKVNVEGFLVSSSHPPAPLEWLTR
                     DDIDRLAPVSRDDFIQIEVVRYF"
BASE COUNT          957 a         1518 c         1369 g          896 t
ORIGIN      
        1 atgtaactgg ggcgcagctg aaggtcaggc aacggaattt gttgcggctt atgtagaaag
       61 aaattgcgcc aacagactgc gcacccgacc catgccgttt ttcagggcca gctcgatttc
      121 cgccatggtg atgatgccgt cactgcgccc ggccgccatg ttcaccacca gtgccagaca
      181 ggcgtaattc aggccggctt cacgagccag cgaggcttcc ggcatgccgg tcattccgac
      241 gatgtcgcag ccatcccttt ccagtcggtc cacttccgcc gccgtttcca gacgcgggcc
      301 ctgggtggcc gcgtataccc cgcggttatg cacgaagtgg ccggttttgt ccgccgccgc
      361 cagcaaacca tgacgcagcg attccgtgta gggaaaggaa aagtccacgt ggatgacgtt
      421 gccttcgtcc gcaaaggtga attcacggcc ccaggtgtaa tcgacgatct ggtgcggaat
      481 caccagcgct tcggcgccca tggcggaatt gataccgccg actgcattca ccgcgatgat
      541 atcggtgacg ccggcgtcgc gcagcgcggc gatattggcg cggtaattta cctgatgggg
      601 cggcaggcgg tggcccttgc catgacgcgc caggaacacc acgtcacgct ggccaaaacg
      661 gccgaattcc agtggcgccg acggtgcgcc gtagcgggtg gtcaccgcga gagattcggt
      721 gatctgcaga ccttccagtt cggtcaggcc cgtgccaccg acgattgcga tcaaactcat
      781 tttgctgtcc ttaaaaaatg gcaacaggat caaagccagg ggaattattc gaagttggac
      841 acgccgttaa cccatccctg ggggctgctc cgcggcatcc ttgccgcaga gcgtccaact
      901 tcgaatagtt cccctggctt tgatcctgta agcgttcgta cgttcactgc cccttcacgg
      961 caaaaatgcc gtccgcgttg cgcaggtaac ccttgtagtc catgccataa ccgaaaatat
     1021 agcggtcggg aatttccagg caggtgtagt cggcgcggaa atccgcatcc gccttgcgat
     1081 cgtgctgctt gtccaccagc acaacggatt tcaccgacct ggcaccctgg gattcgcatg
     1141 cctgcatgat cgccgacagc gtcacgcctt catccagaat gtcgtcgacc accagtacat
     1201 ggcgtttggc caggctcggc ggcggcgcca ccttccacag catgtcagct ccgcccgaca
     1261 gaccgctgcc ataccggctt acatgcacat attccagttc cagcggaaac tgcagctgca
     1321 tcagcaactg cgcgccaaat accatgccgc ccttcatcac caccagtacc agcggattct
     1381 tgtcccccag atcggcgctg atcgtctggg cgatgcgggt gatggaagcc tgcacctgct
     1441 gtgctgtcca cagacaatcg gcttccttcc agaccgtctg aacttcggcg acggatacgc
     1501 tcatgttgat tcctcttgaa tgatggcaaa aaacgcgggc aattgtgcca cagcggaaaa
     1561 ttcctgttga tgctttttaa ccggccacgc cttgacccgc catgtcggcc tgcacggcgt
     1621 gatagtgcgc atcggccagc agctggctcc agtccggccg gcgccgcgcg cgcatgcgct
     1681 gcaaccgcgc ctgattcacc actgccggat gctgttccag atattcaatc accgcctgcg
     1741 cgcccgccgg gttgttgcag accagcacca tgtcgcaacc ggcattcagt gcagcctgcg
     1801 cgcgctggcc gtagtcgccg gccacgctgg caccttccat cgtcagatca tcgctgaaga
     1861 taacaccgtc aaattgcagt tctgtgcgca agacggtctg cagccagtgg cgggaaaagc
     1921 cggcaggatt cgggtccacc tgcgggtaaa tcacgtgggc aggcatcacg gcgtccagtt
     1981 gcccggccag ctgggaaaag gtttgcagat cgcaggcggc aatgtcggac caggagcgct
     2041 cgtccaccgg aatggcatgg tgggaatcgg cctccaccca gccgtgaccg gggaaatgtt
     2101 tgccggtggc agccatcccg gcctcgtgca tgccgtcgat ataggcttcc gccagcacca
     2161 cgacaatcgc tggatcaccg tgaaaggcgc gcgtaccgat cacgctgctg cggcccacgt
     2221 tcaggtccag caccggtgca aaggaaatgt cgaatcccag cgccagcatt tccgacgcca
     2281 tcagccagcc ccactcacgt gcgctggcaa tggcggcttt ggggtcggca tcaaaacgtt
     2341 cacccagctg cgccagcggc ggcaacttgg tcacgccctg ctttacccgc tgcacccggc
     2401 caccttcctg gtccaccgcc agaatcaggt cgggccgcac cgcgcggatc gatgcaatca
     2461 gctcacgcac ctgctccggc tgcacgatgt tgcgggcaaa caggatcacg ccgcccacgg
     2521 cggggtgttg cagacgctgc gcttcataat ccagcagcgc ggttccttcc agatccagca
     2581 tcagggggcc aaggggcatt gcgggtgtga ctcctgcaca aacgtcgaaa gtgacaataa
     2641 aaatgacaat caggcttgcg tgatgatgac aggagtgccg accggcacgc gctcgaacag
     2701 gtccagcaca tcctgattgc gcatgcggat acagccgtgg gagcggggtt cgcccatggg
     2761 ttcagtatcc ggcgtgccat gaatataaat atagcggcgt tgggaatcca cgcagccccc
     2821 gcgattgaaa cctttttctt caccgcacag ccagaggatg cgggtgagta tccagtcacg
     2881 gtggggaaat tcccggccaa tggcgtcgga atagatttcc ccggtgggac gacggccgcg
     2941 aaatacggtg taaatgggct gacctgcgcc gatacgggcg cgaacgcgat ggcgacccag
     3001 gggtgtttga ttcgaccctt cggcattgcc cgcccccttc agggcagtgg acacgggcca
     3061 gcgcgccagc agcttctcgc tgccctgctc gcgcaactcc aggatctgct gatcgataga
     3121 aacgtggatt tccagcctac tcatgagccg cattatgcag gccggcggcc atgaatgcag
     3181 taagccggct cacaataaaa tccaccggcg tgcgttcgcc cgtgtcgttt tcgctcatgg
     3241 cgcgcagggc atcgaagttg gacatggtaa agatcgcggt gcccagagcg aagtgggtgt
     3301 gccagaacag gtcggcggcc gtggcccccg gcagggccgg cttcaggtgt gacgcataat
     3361 gctggaacgc actgccgtag cgctcgccga tgaacttgcg caaatgggcc tgtgactggg
     3421 tatacgccag ccccagcaac cgcatgaacg aggtggcacg gttggaacca cgcccatgga
     3481 tctcgtagac ggaactggtc agcacctgca gtaaatcctc taccgagaga cgctcttttt
     3541 ccgcctgcaa ctggttgagc tggcgttcga tttcgtccac caggggatcg aggaagcgca
     3601 cgaaaaccgc ctgaatcagc gatttttttg aaccaaaatg gtaattcacc gccgccagat
     3661 tcaccccggc cgcgctggtg atggtgcgca gtgaggtttc cgcaaagccg cgttcggcga
     3721 acagcacttc cgccgcatcc agaattcgtt cgactgtttc tttttgtgcc atgaaagggt
     3781 ttcctgctaa acgcccgttt gaaacttacg tttgcagagg ggcgctgtca agaatctgcc
     3841 ctagaagtaa cgcaccactt cgatctggat aaagtcatcc cggctgaccg gcgccaggcg
     3901 gtcgatgtcg tcccgggtca gccactccag cggtgccggc ggatggctgc tggacaccag
     3961 gaagccttcc acattcactt tccaggcatc cccgatacga tggtgaccct cgatcagcca
     4021 gagccgctcg cgggtggcgg ggtcgtaaac cacccccagc aggaaatcgg tgtccgcctc
     4081 gttgttgagg gccagccggg tgcccagcgc cacatcatcg ttcaggtagg tgggcgcctc
     4141 ggcaccccgc tcgtcccagt tgtattccgc caccaggccc aggtctgcgg cggtgtccag
     4201 ccaaccttca agggtgtatt caaagccgaa cgcggtcgcg gcataccggc caccctcctc
     4261 accggaccgg gtcagggcct ccagcttcca cagccagaat tcatgggtgt actgcacctc
     4321 caccgccgcc tgcgcgatct gcggataata aggcaccagc ttgaaatcgg gcgtcaggga
     4381 attgaattcg ggatcgcgcc cggtaccggc aaacagcgac gccgccagtt cccaggcgtc
     4441 gacggaccgc gtccagcgca gggccacatc cagatgctga tcctcttcac ccgattcata
     4501 ttccgccgcg tcttcagcca cctccagcgg cagccggaaa cggccgtcct cgccgggaaa
     4561 aatccgctcg cgaaaacccg ccagcaggta cagatcgaca atcccccagt cctgcaccag
     4621 cgacaggttc accatgggct ggcccaggta agcctcaccg tccggccgtt ccgccagatc
     4681 gacctgattg atgatatcca ccagatggtt gaactccacc acgccccaga acaccgtgcg
//
LOCUS       DASUEG010000396         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_669121, whole genome shotgun sequence.
ACCESSION   DASUEG010000396 DASUEG010000000
VERSION     DASUEG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669121"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1074
                     /locus_tag="VFX11_07285"
     CDS             286..1074
                     /locus_tag="VFX11_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938460.1"
                     /translation="MSAPMPPRGLPGTGGGPNTSDLASSEGKPGRSTGTPVHVEIRNL
                     SKVYDGKKGLQVTSFQIRSGESVAIIGHNGAGKSTLLKILAGWVIPDTGAATVEGIDL
                     KHRSALARKLGFVPEKTNLFDFFSVAYNLTLFARLFHVPDARVEETLVQFELLSFRRN
                     RIDQLSKGLRQRVSIGRALLADPPMLLLDEPTSGLDFDMTRHIYRLLGEIHSSGKTIL
                     FTSHRPEEIRALATRIIVLHQGGLVFDGTPAEYFASGIHLNLYA"
     gene            1086..>2002
                     /locus_tag="VFX11_07290"
     CDS             1086..>2002
                     /locus_tag="VFX11_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4938461.1"
                     /translation="MTLTLNDLAIAFRNKTLQLVLLIPLFVFFSLEMVDQGDSGLQRV
                     NIGLLKSEHYSQALIGGIESADRAFDVVWLGDIEAAQTALQGKKIDGFLTGSAREPGS
                     VELFVSTKTSLKTLAIVEGISALQDATEGKRKPWIAAIQPLHDSDLQRQTLPTWILML
                     VLLIGFIIVPVQVAEEKEKHLLLGLLQTPIREIEWLLAKLLLGIILTMLAAGLLQLLG
                     RFAFDGSAAVSYLAILIAAGFCFSASGIFLGFLCRSQASARTLGMLVYLPNLLPAALS
                     EFSQKLSSVAPLLPSFHFYMPMKAILLEGG"
BASE COUNT          427 a          566 c          566 g          443 t
ORIGIN      
        1 tccaggcgga caagcagagt tcggccgcgt gcgcgggctg atctccaaga atgccgctgt
       61 cgggaaaccc gttcgctttt gcggattggg atggaccggg tcgatcaggc ccggtgtttt
      121 ttaaatagac atattcaaca aggaaagcaa tcatgattgg tattctggtc gtagtggcac
      181 tggttttcac cattgtttat ctggcacaaa gaacctgagc cagcggtgtt ggtagcttcc
      241 ggttcaatgc ttcaccggac tgaggacaat ggaaagccgg cctccgtgtc ggcccccatg
      301 ccgccgcggg gcctgccagg gaccggcggc ggccccaaca catcagatct ggcgtcatcg
      361 gagggaaaac cgggtcgatc cacagggact cccgtacacg tcgaaatcag gaacctctcc
      421 aaagtctatg acggaaagaa aggactacag gtaacgagct ttcagatccg cagcggcgaa
      481 tccgttgcaa tcatcggcca caacggtgcc ggcaagtcga ctctgctgaa aatactggcg
      541 ggctgggtca ttccggatac cggcgcggca acagtggaag gcatcgacct caaacacagg
      601 tcggcactgg cccgaaaact gggtttcgtg ccggagaaaa ccaacctctt cgatttcttt
      661 tccgttgcat acaatctcac gctgtttgcg cgcctctttc atgttccgga cgcccgggtg
      721 gaagagacgc tggtgcaatt tgaactgctg tcgtttcgcc gcaacagaat agaccagctg
      781 tcaaagggac tcaggcagag ggtcagcatc ggtcgcgcac tgctcgccga cccaccaatg
      841 ctgcttctgg atgaacccac ctcaggcctc gattttgaca tgacaaggca tatttatcgt
      901 ctgcttgggg aaattcactc gagcgggaag acaatcctgt tcacttcgca ccgacccgaa
      961 gaaatcagag cgctggcgac acgaatcatc gtcctgcatc agggcggtct ggtatttgac
     1021 ggcactcctg ccgaatattt cgcatcgggc attcacctga atctttacgc atgatgcgaa
     1081 atgttttgac actgacgctg aacgatcttg ccatcgcctt caggaacaag acgctacaac
     1141 tggttctcct cattcctctg ttcgtgttct tttccctgga aatggtggac cagggtgatt
     1201 ccggattgca acgagtcaat atcgggttgc tcaagagcga gcactattcc caggcgttga
     1261 tcggcggtat tgaatcggct gatcgagcct tcgacgtggt atggctgggc gatatcgaag
     1321 cagcgcaaac cgcgttgcaa ggcaaaaaga ttgacggctt tctgaccggt tccgcgcgcg
     1381 agcccgggag tgtggaactg ttcgtgtcga cgaaaacctc gctgaagacc ctcgccatcg
     1441 tcgagggtat ttcggcgctc caggatgcca ccgaagggaa acgcaaacca tggatcgccg
     1501 cgatccagcc gctccatgac agcgacctcc agaggcagac gttgccgacc tggattttga
     1561 tgctggtctt gctcatcggc ttcatcatcg tcccggtgca ggtcgccgag gagaaggaaa
     1621 agcacctgct gctggggctc ttgcagacac ccatccgcga aatcgagtgg ctgctggcga
     1681 aactgttgct cggaataatc ctgacgatgc tcgccgccgg attgctgcag ttgctgggca
     1741 gattcgcatt cgatggcagc gccgcggtca gctacctggc gatcctcatc gctgctggat
     1801 tctgtttcag cgcgagcgga atctttctcg gctttctgtg ccgcagccag gcaagcgcac
     1861 gcacgctcgg gatgcttgtc tatcttccca acctgctccc ggcggcactc tcggagttct
     1921 cccaaaagct cagcagtgtg gctccgctgc tgccttcatt tcatttttac atgccgatga
     1981 aggcgatcct gctggaaggc gg
//
LOCUS       DASUEG010000397         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2081344, whole genome shotgun sequence.
ACCESSION   DASUEG010000397 DASUEG010000000
VERSION     DASUEG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081344"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFX11_07295"
     CDS             <1..782
                     /locus_tag="VFX11_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX4938462.1"
                     /translation="IMLVLIGIVPAQFALNLESSKYEVARARDAVTHLAEFYEEKHDV
                     LAHYLALGKAGRSDLPNEFKCDPQQTEATLNALHNALLEIRTFADLTPQARVDVRRYL
                     LCLDDSAKKVLKLNHLNSSEKKDLDRLRKDLTGSTEYAPFEVVLAVALALGLGTMFGW
                     KRVVLTVGEKIGKQGMTYAQGMSAQVTAAMAIGMANIFSLPVSTTHVLSSGVAGSMVA
                     NKSGIQKETVTTILLAWVLTLPATIFLSAAIYYVANMFFVQ"
     gene            874..2073
                     /locus_tag="VFX11_07300"
     CDS             874..2073
                     /locus_tag="VFX11_07300"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4938463.1"
                     /translation="MMIEPSETAAAQAQWQQGFALLCWSLQSGPGNDADFKRLAQAIT
                     DWEPFIQQLRWHRTGALTWRRLAPYRAQLPDAVGTALQQCRQQITALAMKHSAHVLQL
                     THLFNAHQIPFIVLKGVSLSLQLYGDPARRFSKDIDILVREADVDAAHQALLGNGFHR
                     TYPGETLPDTVLHHYRQFKKDCTYRHERDGTLLELHWRFDNNPHFLPLQHFNPFDHIE
                     HISLSGVPVPVLTAEHNTLYLIMHAAHSSWARLSWLTDIATLLQKPLDWRWIIDTARA
                     LHIEPVVWVTLQLAHRLLQVPVPDVPCRASWRARWVLNGMQQTLQQARYPSNTVRMLL
                     PFLFCNRPTFWLFHLRRNMAISLNDMALLPLPRALFFLYYPLRPFLWGWRRTVGPLDL
                     VSARRVP"
     gene            2070..3830
                     /locus_tag="VFX11_07305"
     CDS             2070..3830
                     /locus_tag="VFX11_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938464.1"
                     /translation="MNPLPLPGLRIPFTPLWHLLRDEKRWPLARLALLMLLTAVAEGV
                     GLLLLVPIIDQVQLGTASANPLVNQLVQAMRWLGIPAGLGGLLLVFLGLVALREILRF
                     QRDTETLRLQTQVIDHHRTRCFERIVRAEWALVSSQPQAYYSNILITDINRIGQGLRF
                     ALNALSTLMLMMISLVAALTLSPAMTLVALVSVLVLLAVLGKFRARAAALGHELGTSN
                     RAIHGCVQESLSGLKLAKLLAGETRLLERFRHGIADLRTRQLQFQRGSAAAAAALQLG
                     GATLLVLFLYGGVRIWHVPLAELAVLALILARLLPLFSGLHQNTLQWLHALPAAQDIL
                     ALQHTHRIAAEPVPPPAGSRWPVQQHILVDNVSVQYDTRQLPALDQVRILLPAGRITA
                     ITGPSGAGKSTLVDVLTGLIVPTRGSLRIDGVLLQPEQRLHWRRAVACVQQDTFLFNT
                     SIRENLLWARPDASDADLREALVRAAADFVLALPAGLDTVTGERGLQLSGGERQRIAL
                     ARALLVQPSLLILDEATSALDAQNEQRIATVLEHLRGQITVLIISHRPALLKHADQVV
                     ELELGRVRTIIGQEHPAVMT"
     gene            3881..4174
                     /locus_tag="VFX11_07310"
     CDS             3881..4174
                     /locus_tag="VFX11_07310"
                     /inference="COORDINATES: protein motif:HMM:NF033536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis PqqD family
                     chaperone"
                     /protein_id="HEX4938465.1"
                     /translation="MPEKIGPNSVITRSTSVIDSDIDGETVMMSLQAGQYFGLEGIGQ
                     RIWHLLESENRLQDICRRLVQEYEVSEAQCERDVRAFLTELLEQDIVAVREPA"
     gene            4175..>4364
                     /locus_tag="VFX11_07315"
     CDS             4175..>4364
                     /locus_tag="VFX11_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938466.1"
                     /translation="MGGRARRFWQLPHRHKRWLLELVLLSALAKIAIAFIPLRYLVRY
                     LGQPHHNRLLSVPVTPDQQ"
BASE COUNT          812 a         1319 c         1381 g          852 t
ORIGIN      
        1 tgatcatgct ggtgctgatc gggatcgtgc cggcgcagtt cgccctcaat ctggaaagca
       61 gcaagtacga agtcgcacgc gcccgcgatg cggtgacgca tctggcggaa ttttacgaag
      121 aaaagcacga tgtgctggca cactatctgg cgctgggcaa ggccggccgg tcggatctgc
      181 ccaacgagtt caagtgcgac ccgcagcaga ccgaagccac cctcaatgca ctgcacaatg
      241 cactgctgga aatcaggacg ttcgcggatc tgacgccgca ggcgcgggtg gacgtacgtc
      301 gctatctgct gtgtctggat gattcggcaa agaaggtatt gaagctgaat cacctgaatt
      361 catccgagaa aaaggatctg gaccgtctgc gcaaggatct gaccggcagc accgaatatg
      421 caccgtttga agtggtgctg gcagtggcgc tggcactggg cctgggcacc atgtttggct
      481 ggaagcgcgt ggtgctgacg gtgggcgaga agatcggcaa gcagggcatg acgtatgcgc
      541 agggcatgag cgcgcaggtg actgccgcga tggcgatcgg catggccaat attttcagcc
      601 tgccggtgtc gaccacgcat gtgctgtcat ccggcgtggc cggctcgatg gtggcgaaca
      661 agagcggcat ccagaaggaa accgtgacca cgattctgct ggcgtgggtg ttgacgctgc
      721 cggccaccat ttttctgtcg gctgcgattt attacgtggc gaatatgttt tttgtgcagt
      781 agccaggcgg catatttgag cagcatttga ccagcttcat ccctccccgt ggttttgact
      841 acactcaaat gggaaccaag ggggatcaag ggaatgatga tcgagccttc cgaaacagcg
      901 gctgcccagg cgcaatggca acagggtttt gcactcctgt gctggagcct gcagagcggc
      961 cccggtaacg atgcggactt caagcggctg gcgcaggcca tcacggattg ggagcccttc
     1021 attcagcagt tgcgctggca ccgcaccggc gcactgacct ggcggcggtt ggcgccgtat
     1081 cgcgcgcaac tgccggatgc ggtcgggaca gccctgcaac aatgccggca acagatcacc
     1141 gccctggcca tgaaacacag tgcgcacgtc ctgcaactga cccacctgtt caacgcccac
     1201 cagattccct tcatcgtgct gaaaggcgtt tcgctgtcgc tgcaattgta tggcgatccc
     1261 gcccgccgct tctccaaaga catcgacatt ctggtgcgcg aagcggatgt ggatgccgcc
     1321 catcaggcac tgctgggcaa cggcttccac cgcacctatc caggtgaaac cctgcccgac
     1381 acggtattgc accattaccg tcagttcaaa aaggattgca cctaccgtca cgagcgggac
     1441 ggcaccctgc tggagctgca ttggcgcttc gacaacaatc cgcattttct gccgctgcaa
     1501 cacttcaatc cgttcgatca cattgaacat atttccctgt cgggcgtgcc ggttccggtg
     1561 ctgacggccg agcacaacac gctttatctg atcatgcatg cggcccattc cagctgggcg
     1621 cggctgtcgt ggctcaccga catcgccacg ctgctgcaaa agccgctgga ctggcgctgg
     1681 atcatcgata cggcccgcgc gctgcacatc gaaccggtgg tgtgggtgac attgcagctg
     1741 gcgcaccgtc tgctgcaggt gccagtgccg gatgtaccct gccgcgccag ctggcgtgcg
     1801 cgctgggtgc tgaatggcat gcagcagacg ctgcagcagg cgcgctaccc cagcaacacc
     1861 gtgcgcatgt tgttgccgtt tctgttctgc aatcgcccga ccttctggct gtttcacctg
     1921 cggcgcaaca tggcgatttc gttgaacgac atggcgctgt tgccgctgcc acgcgcgctg
     1981 tttttcctgt attacccgct gcggcctttt ctgtggggct ggcgccgcac cgtgggtccg
     2041 ctggatctgg tgtccgcacg gcgcgtgcca tgaatccatt gcccctgccc ggtctgcgca
     2101 tcccattcac accgctgtgg catttgctgc gcgacgaaaa acgctggccg ctggcccggc
     2161 tggcgttgct gatgctgctg actgccgttg ccgaaggtgt cgggctgttg ctgctggtac
     2221 ccatcatcga tcaggtgcag ctgggcactg cgtcggccaa tccgctggtt aaccaactgg
     2281 tgcaggccat gcgctggctg ggaatacccg ccgggctggg tggattgctg ctggtatttc
     2341 tggggctggt ggcgctacgg gaaatactgc gcttccagcg cgacacggaa acgctgcgcc
     2401 tgcaaacgca agtgatcgat catcaccgca cccgctgttt cgaacggatc gtgcgcgcag
     2461 agtgggcgct ggtcagcagc cagccgcagg cgtattacag caacattctg atcaccgaca
     2521 tcaatcgcat cggccagggt ttgcgcttcg cgctgaacgc gctgtccacc ttgatgctga
     2581 tgatgatctc gctggtggcg gcactgacgc tgtcgcccgc catgacgctg gtggcgctgg
     2641 tcagtgtgct ggtgctgctg gcagtgctgg gaaaattccg cgcgcgggct gcggcactgg
     2701 gacacgaact cggcaccagc aaccgcgcca ttcacggctg tgtgcaggaa agcctgagcg
     2761 gcctgaaact ggcgaaactg ctggcgggcg aaacgcgcct gctggaaaga ttccggcacg
     2821 gcatcgcgga tctgcgcacg cggcaactgc agttccagcg cggcagcgcg gcagcggcgg
     2881 cggcactgca gctgggcggt gccacgctgc tggtgctgtt tctgtatggt ggcgtgcgga
     2941 tctggcacgt gcccctggcg gaactggcgg tgctggccct gattctggcg cggctgctgc
     3001 cgctgttttc cggcctgcat caaaacacgc tgcagtggct gcatgccctg cccgccgcgc
     3061 aggatattct ggcgcttcag cacacacacc ggatcgcggc ggaaccggtg ccaccaccgg
     3121 ctggatcgcg ctggcctgtg caacaacaca ttctggtgga caacgtgagc gtgcagtacg
     3181 acacgcgcca gctgcccgcg ctggatcagg tgcgcattct gttgccggcc ggacggatca
     3241 ccgccatcac cggcccttcc ggcgcgggca aaagcacatt ggtggatgtg ctgacgggcc
     3301 tgatcgtgcc cacccgcggc agcctccgca ttgacggcgt gctgctgcaa ccggaacaac
     3361 gtttgcactg gcgccgcgcg gttgcctgcg tgcaacagga cacgtttctg ttcaacacct
     3421 ccattcgtga aaacctgttg tgggcgcgtc cggatgccag cgatgcagac ctgcgcgagg
     3481 cactggttcg cgcggcggcc gatttcgtgc tggcgctgcc ggcagggctg gacacggtca
     3541 ccggcgaacg tggcctgcaa ttgtccggtg gcgaacggca acgcatcgcg ctggcccggg
     3601 cgctgctggt acagcccagc ctgctgattc tggatgaagc caccagcgcg ctggatgcac
     3661 agaatgaaca gcgcattgcc accgtgctgg agcacctgcg tggacagatc acagtgctga
     3721 tcatcagcca ccgccccgca ttgctcaagc acgccgatca ggtggttgaa ctggaactgg
     3781 gccgagtgcg caccatcatc ggtcaagagc atcctgccgt catgacatag acattcaatc
     3841 gacaaaacca caacccgccg caatggacaa ggagaatccc atgccagaga agatcggacc
     3901 caacagcgtg atcacccgca gcaccagcgt aatcgacagc gatatcgatg gcgaaaccgt
     3961 gatgatgagc ctgcaggcag gccagtattt tggtctggag ggcattggcc agcgcatctg
     4021 gcatctgctg gaatcggaaa accggttgca ggatatttgc cggcgactgg tgcaggagta
     4081 tgaagtgagc gaggcgcagt gcgagcgcga tgtacgggcc tttctcactg aactgctgga
     4141 acaggacatt gttgcggtgc gggagccggc ctgaatgggt ggacgggcgc gacgcttctg
     4201 gcaactgccg caccggcaca aacgctggct gctggagctg gtgctgctga gcgcgctggc
     4261 gaaaatcgcg attgctttta tcccgctgcg gtatctggtg cggtatctgg gccagccgca
     4321 tcacaatcgt ttgctgtcag tgccggtaac acctgaccag cagc
//
LOCUS       DASUEG010000398         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1856173, whole genome shotgun sequence.
ACCESSION   DASUEG010000398 DASUEG010000000
VERSION     DASUEG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856173"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX11_07320"
     CDS             <1..480
                     /locus_tag="VFX11_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX4938467.1"
                     /translation="KDFLQQYLPARPGRIVTDQGETIGEHQGLMYYTMGQRQGIGIGG
                     VAHKGEAPWYVLGKNLETNELIVGQGTDHPWLFTSRLSTSDVNWVTGEPALPMRCRAK
                     TRYRQPDQDCTVFARDNGNYEVIFDQPQRAVTPGQSVVFYVDDVCLGGGVIEHAWNP"
     gene            493..1128
                     /gene="hflD"
                     /locus_tag="VFX11_07325"
     CDS             493..1128
                     /gene="hflD"
                     /locus_tag="VFX11_07325"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEX4938468.1"
                     /translation="MIDREQQKALALAGLFQSAALVQQLARTGEITGDAYTALIDSIF
                     LLDPQSVEEVYGGVKGAKLGIDTLLMIMKEKETARYADAIRYSIGMLQVEKLLVRNAD
                     INSILRSRLEQLSTQRKHFESPTHSSIIARLNDIYLDTLAKFKFRIQVNGDPKHLQRE
                     ENAAKIRAILLAGVRSALLWRQLGGSRLQFAFGKRRLVAALEDLQQKAALS"
     gene            1218..2585
                     /gene="purB"
                     /locus_tag="VFX11_07330"
     CDS             1218..2585
                     /gene="purB"
                     /locus_tag="VFX11_07330"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX4938469.1"
                     /translation="MELNALTAISPVDGRYGSKTQSLRGIFSEFGLIRFRVLVEVRWL
                     QQLARHDGIAEVPVFSHQTQQLLNSIVDDFSVEDAQRIKDIERTTNHDVKAVEYFLKE
                     KTAGNTELEYVSEFIHFACTSEDINNLSHGLMLKAGRDEVLVPMLQKLVDAIAGLAHR
                     YADMPMLSRTHGQTASPSTMGKEMANVAARLRRQLDQIRQVPLLGKINGAVGNYNAHL
                     SAYPEIDWETTAQQFVTSLGLEWNPYTTQIEPHDYIAELYDAIARFNTILIDFDRDVW
                     GYISLGYFKQKTVAGEIGSSTMPHKVNPIDFENSEGNLGIANAILHHLASKLPISRWQ
                     RDLTDSTVLRNLGVGLAHGLIAYEASLKGIGKLEINEQRLADDLDNAWEVLAEPIQTV
                     MRRYRIEKPYEKLKELTRGKAITRTTLMAFIDTLEIPEQEKVRLRALSPASYTGNAAQ
                     QAKKI"
     gene            2588..>3158
                     /locus_tag="VFX11_07335"
     CDS             2588..>3158
                     /locus_tag="VFX11_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4938470.1"
                     /translation="MSAKTFEILGGRAPDAFLRDYWQKKPLLIRNAFPGIEGLLEPDE
                     LAGLALEEEIESRIIIERSPIDWELRHGPFTEKTFKNLPQTHWTLLIQALDHYVPQIA
                     DLLEHFDFIPNWRIDDIMASFAPFGGSVGPHYDYYDVFLIQAHGQRRWQLGQTCDDST
                     ALLPDLPIRILRDFQPDQEWVLNPGDMLYL"
BASE COUNT          697 a          968 c          920 g          573 t
ORIGIN      
        1 aaggatttcc tgcagcagta tctgccggca cggccgggca ggatcgtcac cgatcagggt
       61 gaaaccatcg gcgagcatca gggcctgatg tattacacca tgggtcagcg ccagggcatc
      121 ggcattggcg gcgtggccca caagggcgag gcaccctggt atgtgctggg gaaaaatctg
      181 gagaccaatg aactgatcgt gggccagggc acggatcatc cctggctgtt cacgtcgcgg
      241 ctgtcgacgt ccgatgtgaa ctgggtgacg ggggaaccgg ccctgcccat gcgttgccgc
      301 gccaagacgc gctaccgtca gcccgatcag gactgcacgg tgttcgcccg tgataacggc
      361 aactatgaag tgattttcga ccagccccag cgcgcagtga cgcctgggca gtccgttgtt
      421 ttttatgtcg atgacgtctg cctcggtggt ggcgtcattg agcatgcctg gaatccttga
      481 gggagcattg atttgatcga tcgcgaacaa caaaaagcgc tcgcgctggc cggcctgttc
      541 cagtccgccg ccctggtgca gcagctggcc cgcaccggtg agatcacagg tgatgcctac
      601 accgcgctga tcgacagcat tttcctgctg gacccgcaaa gcgtggagga agtctacggc
      661 ggcgtgaagg gcgccaagct cggtatcgat accctgctga tgatcatgaa ggaaaaggaa
      721 actgcccggt acgccgacgc aatccgttac agcatcggca tgctgcaggt ggaaaagctg
      781 ctggtgcgaa atgcggatat caacagcatc ctgcgctcgc ggctggagca gctcagcacc
      841 caacgcaaac acttcgaatc gccgacgcat tccagcatca tcgcccgact gaacgacatc
      901 tatctggata ccctggccaa attcaaattt cgcatccagg tcaacggcga ccccaagcat
      961 ctgcagcgcg aggaaaacgc cgccaagatc cgcgccattc tgctggcagg cgtgcgctct
     1021 gccctgctct ggcgtcagct gggtggcagc cgtctgcagt tcgcctttgg caaacgccgg
     1081 ctggtggctg cactggaaga cctgcagcaa aaggccgcgt tgtcgtgatc gctgcaaccg
     1141 cagcctgaga cgcccggctg attcatcatg caacccattt catttttctg aagtctgatt
     1201 ttccacagga gcccgtcatg gaactcaacg cactcaccgc catctccccg gtcgacggcc
     1261 gctacggcag caagacccag tccctgcggg ggattttcag cgaattcggc ctgatccgtt
     1321 ttcgggtgct ggtggaagta cgctggctgc agcaactggc gcgccacgac ggcatcgccg
     1381 aagtgcctgt tttttcccac cagacccagc agctgctgaa cagcatcgtg gatgattttt
     1441 cggtggagga cgcgcagcgc atcaaggata tcgagcgtac caccaaccat gacgtgaaag
     1501 cggtggaata tttcctgaaa gaaaaaaccg ccggcaatac cgagctggaa tatgtcagcg
     1561 agttcatcca cttcgcctgc acatcggagg acatcaacaa tctgtcccac ggcctgatgc
     1621 tgaaagcggg tcgcgatgaa gtgctggtgc ccatgctgca gaaactggtg gacgccattg
     1681 ccggactggc gcaccgctac gccgacatgc cgatgctgtc gcgcacccat ggccaaaccg
     1741 cttccccttc caccatgggc aaggagatgg cgaacgtggc agcgcggctg cggcgtcagc
     1801 tggaccagat cagacaggtg ccactgctgg gcaagatcaa cggcgcggtg ggcaactaca
     1861 acgcacacct gagcgcgtat cccgaaatcg actgggagac caccgcacag cagttcgtga
     1921 ccagcctggg tctggaatgg aatccgtata ccacccagat cgagccgcac gattacatcg
     1981 ccgaactgta tgacgcgatc gcccgcttca acaccatcct gatcgatttc gaccgcgatg
     2041 tgtggggcta catttcgctg ggctatttca agcagaaaac cgtggccggc gagatcggtt
     2101 catccaccat gccgcacaag gtgaatccga tcgacttcga aaattccgaa ggcaacctgg
     2161 gtatcgccaa tgcgatcctg caccacctgg cgagcaagct gccgatctcg cgctggcagc
     2221 gtgacctgac cgattccacc gtactgcgca atctgggcgt tggtctggcc cacggtctga
     2281 tcgcgtacga agcctcgctg aaagggatcg gcaagctgga aatcaacgag cagcgtctgg
     2341 cggacgatct ggacaatgcc tgggaagtgc tggcggagcc gatccagacg gtgatgcgcc
     2401 gctaccgcat cgaaaaaccc tatgaaaaac tgaaagagct cacccgtggc aaggccatca
     2461 cccggacaac cctgatggct ttcatcgaca cgctggaaat tcccgaacag gaaaaagtgc
     2521 ggctgcgggc actgtcgccg gcatcctaca ccggcaacgc ggcgcagcag gcgaagaaga
     2581 tctgacgatg agtgcaaaga catttgaaat cctgggcggc cgcgcgccag acgcattcct
     2641 gcgggattac tggcagaaaa aaccgctgct gatccgcaac gcgtttcccg gcatcgaagg
     2701 gctgctggaa ccggacgaac tggcagggct ggcgctggaa gaggaaatcg aatcccgcat
     2761 catcatcgag cgcagtccga tcgactggga actgcgccac ggacccttta cggaaaagac
     2821 gttcaaaaat ctgccgcaaa cccactggac cctgctgatc caggccctgg accattacgt
     2881 gccccagatc gccgatctgc tggaacattt cgatttcatc cccaactggc gcatcgacga
     2941 catcatggcc agcttcgcgc ccttcggtgg cagcgtcggc ccgcactacg attattacga
     3001 cgtgttcctg atccaggccc acggccagcg ccgctggcaa ctgggccaga cctgcgacga
     3061 cagtacggcc ctgctgccgg acctgccgat ccgcatcctg cgggatttcc agccggacca
     3121 ggaatgggtg ctcaacccgg gcgacatgct gtacctgc
//
LOCUS       DASUEG010000399         5500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1335403, whole genome shotgun sequence.
ACCESSION   DASUEG010000399 DASUEG010000000
VERSION     DASUEG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335403"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="cbiD"
                     /locus_tag="VFX11_07340"
     CDS             <1..744
                     /gene="cbiD"
                     /locus_tag="VFX11_07340"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HEX4938471.1"
                     /translation="AINPVPRRNIEQNVREVAAELLAQAGLEVIISVPEGEAMAKKTL
                     NDRLGILGGISILGTTGIVRPYSTAAFRASVVQGIEVAAAQGQDTVVLTTGGRSEKFV
                     MQELPQLAPACFIQMGDFLSYALDAARRCKLKTVVIGGMVGKLTKMAQGETITHANRN
                     PVDTELLAELAQSLGVDADVCADIRNAETARYASERMEALGLSLAFHQALAQRVVQTL
                     AARYPRQFRIRVMVCDFDGKRITEAEGNE"
     gene            737..2068
                     /gene="cbiE"
                     /locus_tag="VFX11_07345"
     CDS             737..2068
                     /gene="cbiE"
                     /locus_tag="VFX11_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX4938472.1"
                     /translation="MNEAEQFPLRCRIIGVLDDGAASLHASALQHVRQADLVIGATRT
                     LQLFAAEFSVGCETRDLNGRLSLVPEWIRQALSDQLKVVVLASGDPLCHGIAGFLVAH
                     LCIEACEILPNVSTLQLACARIGLPWQDVKIASVHARDAGEWHIGAGLHHGLAPLLRD
                     ISRHDRLAILTSPDNTPDRIARMLVQENMADDFRIAVAERLLLPDENIVTAVSVRDAA
                     QRRFADPNVVLLWRNAPQPNPVLFGLADNGYQQRQPEKGLITKREVRAVSLARLQLKA
                     GSIVWDIGAGSGSVGLEAARLCPQGYVYAIEKNTGDAENAAANRVQLGIHNYHLRIAK
                     APDGLQEWPDPDAVFIGGSGGELVHLIELCLTRLQPGGWLVMNLVTVENLAVAMETLK
                     AMQVQWDVTQLQTSRSKPILDMHRFAAENPVWIVAAQRPLAADSNQAVTIS"
     gene            2065..2910
                     /gene="cobI"
                     /locus_tag="VFX11_07350"
     CDS             2065..2910
                     /gene="cobI"
                     /locus_tag="VFX11_07350"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX4938473.1"
                     /translation="MTGIEINHSIHTGKLMGVSLGPGNPGLITRRAWQSLHGDAVWTY
                     PVRRKNGRSFALDIALRAGTVLPADSIPLVFPMTHDAEKLARHWLLAAETVLQLLYLG
                     RDVVFLVEGDASTYSTWNHLSRTVRALDDRIELETIAGVSSFQAAAAQLRMPLADVDD
                     TVAILPAAYGVAAVERLLPEFDTLVLLKVKPLLDDLIDLLARRDLLQHACFVEKVGAE
                     EERVIRDIVSLRGEKVNYLSLLLVRNPHRNKGERLRGCRKKADNENINGLAAIAVPQV
                     KEAST"
     gene            2907..3686
                     /locus_tag="VFX11_07355"
     CDS             2907..3686
                     /locus_tag="VFX11_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis central domain-containing
                     protein"
                     /protein_id="HEX4938474.1"
                     /translation="MNTDINRASDYSGTTLQPRIALIAITRHGARQVNALAPALPAAS
                     ICIAEKVAGELGALPNSLHMYNGALRDEIGPLFSQFDQLVFFVSLGAVVRLIAPFLKS
                     KDEDPGVLVVDDAGEFVIPVLSGHVGGANACAEFIADLLGARAVLTTASDVGKTIPVD
                     ILGRALGWKLEAPKINVTRVSADVVNGEPVAFVQEAGSPHWWTRPTPLPSNIHLFNAF
                     EAVDLARFKSVLWVTHRDVSAGVWQSLQERLVVYRPPRDTV"
     gene            3697..4110
                     /locus_tag="VFX11_07360"
     CDS             3697..4110
                     /locus_tag="VFX11_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX4938475.1"
                     /translation="MNASSAFRVAIGIGCDRGASLKTFAAALDQALALCHCERRQIAV
                     VASIDLKRNEVALLDFAQAHGWPLQFYPAPALAQVSVPNPSETVRRHTGTPAVAEAAA
                     LLAADTHAADLLVEKFKLKGEDGRNVTISIARMRI"
     gene            4076..>5500
                     /gene="cobJ"
                     /locus_tag="VFX11_07365"
     CDS             4076..>5500
                     /gene="cobJ"
                     /locus_tag="VFX11_07365"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX4938476.1"
                     /translation="MSPSPSRACASEQNKHGKIFLVGLGPGSEQHMTARAREAIAQSD
                     VVIGYSTYIKLVSELLAGKQVIRKGMTEELDRCHEALSAAREGKTVALVSSGDSGVYG
                     MAGPTYEVLFESGWQPDDGVDVEVVPGSSAINSCAALVGAPLTHDFCSISLSDLLTPW
                     PVIAGRLQAAARGDFVVALYNPKSGRRTGQIVEAQRILLQHRNPATPVALVKSGYRRR
                     QSIQLTRLDQMAECDIGMLTTVLIGNSNTFVRHGLMVTPRGYANKYDTDGAVQESRAR
                     ERVHDGEQAGRSLSMGLLGWHEYVRAFLQEKPERTLRDAAAHFRMPLAEILAAVSHVA
                     TDSAPGDWQSAQVSPQALDDLLVWTQDWGPLRAVVRADSGSVAELLLDGDAFTWGEPA
                     QPAASRWLNLVTPHFHLHVNWSNVTQGWLVGHNGDLHSACFLDHTGAQVFSLQPRSVS
                     GAFHFACPFDRAELALRLPLQAAAM"
BASE COUNT         1055 a         1552 c         1805 g         1088 t
ORIGIN      
        1 gccatcaatc cggtgccgcg caggaacatc gaacagaatg tgcgggaagt ggccgccgaa
       61 ctgctggcgc aagccgggct ggaagtgatc atttcggtac ccgaaggcga agccatggcg
      121 aagaaaacgc tgaacgaccg gttgggcatt ctgggtggta tttcgattct gggtaccacc
      181 ggcattgtgc ggccttattc gaccgcagcg tttcgtgcca gtgtggtgca gggtattgaa
      241 gtggcagcgg cgcaggggca ggacacggtg gtgcttacca ccggcggacg ttcggaaaaa
      301 ttcgtgatgc aggaattgcc gcagctggcg ccggcctgtt ttatccagat gggggatttc
      361 ctgagttacg cactggatgc ggcgcggcgc tgcaagctga aaaccgtcgt gatcggcggc
      421 atggtgggca aactcaccaa gatggctcag ggcgaaacca tcacccacgc caatcgcaat
      481 ccggtggata ccgagctgct ggcggagctg gcgcaatccc tcggcgtgga tgccgacgtg
      541 tgcgccgaca tccgcaacgc cgagacggcg cgctacgcca gtgaacgcat ggaggcgctg
      601 gggctcagtc tggcattcca tcaggcgttg gcgcaacggg tggtgcagac gctggcggcg
      661 cgttacccac gtcagtttcg tattcgtgtg atggtgtgtg atttcgatgg caagcggatt
      721 acagaggcgg aaggcaatga atgaagcaga gcaatttccg ttgcggtgcc ggatcatcgg
      781 tgtgctcgac gatggggctg ccagtttgca tgcctcggca ttgcagcacg tccggcaggc
      841 tgacctggtg atcggagcga ctcgtacatt gcaactgttc gcagcagaat tcagcgtggg
      901 gtgcgagacg cgcgatctca acggccgcct gtcgctggta ccggaatgga tacggcaggc
      961 gctgtccgat caattgaagg tggtggtgct ggccagtggt gatccgctct gtcatgggat
     1021 tgccggattt ctggtggcgc acctgtgtat cgaagcctgt gaaatcctgc cgaatgtttc
     1081 cacgttgcaa ttggcctgcg cacggatagg tcttccctgg caggatgtga aaattgcctc
     1141 ggtgcatgcg cgcgacgcag gagaatggca catcggcgcg ggcctgcacc acggactggc
     1201 gccgttgctg cgcgacatca gccggcacga tcggctggcc attctcacca gtcccgacaa
     1261 cacaccggat cgcattgccc gtatgctggt gcaggaaaat atggcggatg attttcgcat
     1321 cgcggtggcc gagcgcctgc tgttgccgga cgaaaatatc gtaacagcag tatctgtgcg
     1381 cgatgcggcg cagcgccggt ttgccgatcc caatgtggtg ctgctgtggc gcaatgcgcc
     1441 gcaaccgaat cccgtgctgt tcggtctggc ggataacggc tatcagcaac gccaaccgga
     1501 aaaaggcttg atcaccaagc gcgaagtgcg agcagtttcg ctggcgcgcc tgcaattgaa
     1561 ggcaggcagc attgtctggg atatcggcgc cggctccggt tccgtggggc tggaggcggc
     1621 ccgtctgtgt ccgcaaggtt acgtgtacgc catcgaaaaa aataccggcg acgctgaaaa
     1681 tgcagcggcc aatcgcgtgc agctgggaat tcacaattac catctgcgca ttgcaaaagc
     1741 gcccgatggt ctgcaggaat ggcctgaccc ggatgcggta ttcattggcg gttccggtgg
     1801 cgaattggtg cacttgatcg agttgtgcct gacacggctg caacccggtg gctggctggt
     1861 gatgaatctt gtcacggtgg aaaatctggc cgtggcgatg gaaacgctca aggcgatgca
     1921 agtgcagtgg gatgtcaccc agctgcagac gtcccgcagc aaaccgattc tggacatgca
     1981 ccgcttcgcg gcggaaaacc cggtctggat cgtggcagcg cagcgaccgc tggcagcgga
     2041 ttccaaccag gcagtgacaa tctcatgaca ggcatcgaaa tcaaccacag tatccacacc
     2101 ggcaaattga tgggtgtttc ccttggcccc ggtaatcccg ggctgattac gcgacgggcc
     2161 tggcaaagcc tgcacggtga cgctgtgtgg acctatcccg tgcggcgcaa gaacggccgc
     2221 agtttcgcac tcgatattgc cttgcgcgca ggcacagtgt tgccggcaga ttcgattccc
     2281 ctggtatttc ccatgaccca cgatgcggaa aagctggcac gacactggct gctggcggcg
     2341 gaaaccgtgc tgcaactgct gtacctgggt cgcgacgtgg tgtttctggt ggaaggggac
     2401 gcgtccacct attcaacctg gaatcatttg tcccgcacag tgcgcgcgct ggacgatcgc
     2461 atcgaactgg aaaccattgc cggggtgtca tctttccagg cggccgcagc gcaattgcgc
     2521 atgccattgg ccgatgtgga cgacacggta gcgatattgc ccgcagcgta cggagtggct
     2581 gcggtggagc gcctgctgcc ggaattcgac acgctggtac tgctgaaagt gaagccgttg
     2641 ctggatgacc tgatcgacct gctggcgcgc cgggatctgc tgcaacatgc ctgcttcgtg
     2701 gagaaagtgg gagcggagga agagcgggtg atacgcgata tcgtcagtct gcggggcgag
     2761 aaggtgaatt acctgtcgtt gctgctggtg cgcaatcctc accgcaacaa gggcgagcgc
     2821 ctgcgcggct gccgcaagaa ggcagacaac gagaacataa acggcctggc tgcgattgct
     2881 gtgccgcagg taaaggaagc atcgacatga atactgacat caacagggcg tccgactatt
     2941 cgggaactac cctgcaaccc cgcatcgcac tgatcgccat cacccgccac ggcgcgcggc
     3001 aggtcaacgc gctggcacct gcgttgccgg ctgcgtcgat ctgcatcgcg gaaaaagtgg
     3061 cgggcgaact gggtgcgttg cccaacagcc tgcacatgta caacggcgca ctgcgggatg
     3121 agatcggtcc gctgttcagc cagttcgacc agctggtgtt tttcgtgtcg ctgggagcgg
     3181 tggtgcggct gattgcgcca ttcctgaaat ccaaggatga agatcccggc gtgctggtgg
     3241 tggacgatgc cggcgaattc gtgatccccg tcttgtcggg ccatgtcggc ggcgccaatg
     3301 cctgcgcgga attcatcgct gacctgctcg gtgcgcgggc ggttctcacc actgcatccg
     3361 atgtgggtaa aaccattccg gtggatatcc tgggccgggc gttgggctgg aaactggaag
     3421 cgcccaagat caatgtcacc cgcgtatcag cggatgtggt gaacggcgaa cccgttgcgt
     3481 tcgtgcagga ggctggctct cctcactggt ggacccggcc tacgccgctg ccatcgaata
     3541 tccatctgtt caatgctttc gaagccgtgg atctggcgcg tttcaaatcc gtgctgtggg
     3601 tgacgcaccg cgatgtttct gccggagtgt ggcaatcact gcaggaacgg ttggtggtct
     3661 accggccgcc ccgtgacact gtatgaatgt gccgacatga acgcaagcag cgcattccgc
     3721 gtcgccattg gcatcggttg tgatcgcggt gcgtcgctga agacatttgc ggccgcactg
     3781 gatcaggcat tggcgctgtg tcattgcgaa cgccggcaaa ttgctgttgt agccagcatc
     3841 gatttgaaac gcaatgaagt ggcgttgctg gatttcgcgc aggcgcatgg ctggccgttg
     3901 cagttctatc cggcgccagc actggcacag gtgtcggtgc ccaatccatc ggaaaccgtg
     3961 cggcgccaca ccggtacacc cgctgtggcg gaagcggcag cgctgctggc ggctgacacc
     4021 catgcagcgg atttgctggt ggaaaaattc aaactcaaag gcgaggacgg caggaatgtc
     4081 accatctcca tcgcgcgcat gcgcatctga acagaataaa cacggcaaga tttttctggt
     4141 gggactcggc ccgggcagcg agcagcacat gacggcgcgg gcgcgggaag ccatcgcgca
     4201 gtccgatgtt gtcatcggct attccaccta tatcaagctg gtgtcggaat tgctggccgg
     4261 caagcaggtg atccgcaagg gcatgaccga agagctggat cgctgtcacg aagcgctgtc
     4321 tgccgcccgg gaaggcaaga ctgtggcact ggtgtcgtcc ggcgacagcg gtgtttatgg
     4381 gatggccggg ccgacgtatg aagtgctgtt cgaatccggc tggcaacccg acgatggtgt
     4441 cgacgtggaa gtggtgcccg gcagttccgc catcaattcc tgcgcggcgc tggtaggcgc
     4501 gccactgact cacgatttct gttcgatctc gctgtcggat ctgctcacgc cctggccggt
     4561 gattgccggc cggctgcagg cagcggcgcg cggcgatttt gtggtggcgc tctacaatcc
     4621 gaaaagtggg cgccgcaccg gccagattgt cgaagcccag cgcatcctgc tgcaacaccg
     4681 caatccggcc acgccggtgg cgctggtgaa atccggctac cgccggcgcc agtcgatcca
     4741 gctcacgcgt ctggaccaga tggcggaatg tgatatcggc atgctgacca ctgtgctgat
     4801 cggtaacagc aatacgttcg tacgacatgg cctgatggtg acaccgcgcg gttacgccaa
     4861 caaatacgac accgatggcg ccgtgcagga aagccgcgcc cgcgaacgcg tgcatgacgg
     4921 tgagcaggcc gggcgttccc tgagcatggg gttgttgggc tggcacgaat atgtgcgcgc
     4981 gtttttgcag gaaaaaccgg aacgcacact gcgcgatgcg gctgcccact tccggatgcc
     5041 gcttgcggag attctggcgg cggtatccca cgttgccacc gacagcgcgc cgggcgattg
     5101 gcaaagcgca caggtctcgc cgcaagcact ggacgatctg ctggtgtgga cacaggattg
     5161 gggcccgttg cgggcagtgg tgcgtgccga ttccggcagc gtggcggaat tgttgctcga
     5221 tggcgacgct ttcacctggg gcgaacctgc acaaccagcc gcatcgcgct ggctgaatct
     5281 ggtgacgccg cattttcacc tgcacgtgaa ctggagcaac gtgacacaag gctggctggt
     5341 cgggcacaac ggcgatctgc acagtgcctg ctttctggat cacaccggcg cgcaggtgtt
     5401 ttcattgcag ccgcgttccg tgtcaggtgc attccatttc gcctgtccct ttgatcgcgc
     5461 cgagctggcg ttgcgcttgc cactgcaggc cgctgcaatg
//
LOCUS       DASUEG010000400         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1657734, whole genome shotgun sequence.
ACCESSION   DASUEG010000400 DASUEG010000000
VERSION     DASUEG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657734"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX11_07370"
     CDS             complement(<1..215)
                     /locus_tag="VFX11_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEX4938477.1"
                     /translation="MIHPQAIVDPKAQLAADVSVGPWTYIGPDVQIDSGTRIESHVVI
                     KGPTRIGKNNRIFQFSSVGEDTPDLKY"
     gene            complement(212..664)
                     /gene="fabZ"
                     /locus_tag="VFX11_07375"
     CDS             complement(212..664)
                     /gene="fabZ"
                     /locus_tag="VFX11_07375"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX4938478.1"
                     /translation="MMNINEIKAILPHRYPFLQVDKVIELVEGEYIVAVRSVTNGEPV
                     FEGHFPNNPILPGVLIIEAMAQAAGLLAFKTMGQTANANKAYVVAAVDEAKFRRPVIP
                     GEQLVIRAYIKSRKRVIWKFGADAYVDRVGGDVVGSAVITCAERDLVA"
     gene            complement(799..1887)
                     /gene="lpxD"
                     /locus_tag="VFX11_07380"
     CDS             complement(799..1887)
                     /gene="lpxD"
                     /locus_tag="VFX11_07380"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX4938479.1"
                     /translation="MSADQGKGNGLTLGELAQQLGAELRGDPAIRIRGLATLGDAQAG
                     QLSFLANPLYRAQLASTSASAVIVAGDMAAECPVACLVVANPYLTYARASHLFNHRPR
                     QPDGVHPTAVIAPDAVLGAGCRIGPHVVIEAGCLLGERVEVGPGTCIGANSRIGNDCV
                     LHGNVTIYHGVVIGERCTLHGGVVIGADGFGFANEKGAWRKIAQLGGVRIGNDVEVGA
                     STTIDRGALRDTIIGDNVILDNQIQIAHNVEIGEGTAIAGCTGIAGSTKIGRYCTIAG
                     AVGIAGHLEIADRVHLTMRSAITNSIDEPGSYSSGTAMSTTAEWRKNAVRFRQLDSMA
                     RRIRQLEARLKALDAAGPAASAPDSSAD"
     gene            complement(1924..2427)
                     /locus_tag="VFX11_07385"
     CDS             complement(1924..2427)
                     /locus_tag="VFX11_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX4938480.1"
                     /translation="MKGFKLLVLAGLLLSSAPVWAEIRIAVVDLNAAVMKSDEARLRA
                     EKLKSTLGQEEADAIALRNQIQKLDEKRQKDSAVMGQDELRRLEKEIADKKIELNFKG
                     QKLQQKGKDASQELQSVMGPKVEKAMKAIVDEKKYDLILQRQAALWVDPKFDITPELT
                     QRINAQK"
BASE COUNT          499 a          809 c          669 g          558 t
ORIGIN      
        1 ttgtatttca gatcgggcgt gtcttcgccc acgctggaaa actggaaaat ccggttattc
       61 ttgccaatcc gcgtcggccc cttgatcacg acgtgggatt cgatccgggt tccggaatca
      121 atctgcacat cgggcccgat ataggtccag ggcccgacac tgacgtcggc tgccagctgc
      181 gccttcggat caacaattgc ctgcggatgt atcacgccac cagatccctt tctgcacagg
      241 taataacagc agaccctacg acatcaccgc caacgcggtc cacataggca tctgcgccga
      301 atttccagat tacgcgcttg cgcgacttga tgtacgcccg aatcaccagt tgttcaccgg
      361 gaatcactgg tctgcgaaac tttgcctcat ccacggcggc cacgacgtag gccttgttgg
      421 cattggcagt ctggcccatg gtcttgaacg ccagcagacc ggcagcttga gccatggcct
      481 cgatgatcag tactcccggc aggatcggat tgttggggaa atgcccctcg aataccggtt
      541 cgccgttggt cacgctgcgc acggctacaa tatattcccc ttccaccagt tcgatgacct
      601 tgtccacctg caggaaagga tagcggtgcg gaaggattgc cttgatctcg ttaatgttca
      661 tcatagttca aacagtttcg cagagggtga attcttgtca ggataaggcc cttcggcatg
      721 gattgcaccc cgacaaatgc aacccgatat ttccccattc ccgcatgacc acacagccac
      781 ttccggcatt tcaccgtatc agtcagcgct gctgtccgga gctgacgccg ccgggcccgc
      841 agcatccagt gccttgagcc gcgcttccag ctgcctgatc cgccgcgcca tgctgtccag
      901 ctggcgaaaa cgcactgcgt tcttgcgcca ttccgccgtc gtcgacatgg cggtgcccga
      961 cgaataggaa ccaggttcat caatgctgtt ggtaatggcg ctgcgcatgg tgaggtgaac
     1021 acggtcggcg atttcgaggt gcccggcgat acccactgct ccggctatgg tgcagtagcg
     1081 gccgattttc gtgctgccag caatgccggt acagcctgcg atggcggtgc cctcgccgat
     1141 ctccacgttg tgggcgatct ggatctggtt atcgagaatc acgttgtcgc cgataatcgt
     1201 gtcgcgcagg gcaccccggt cgatggtggt actggcgccg acttcaacgt cattaccaat
     1261 ccgaacacca cccagctgcg caatcttgcg ccaggcgccc ttctcattgg caaaaccgaa
     1321 accatcggca ccaatcacca cgccaccatg cagtgtgcag cgctcgccaa tcaccacgcc
     1381 gtgatagatc gtcacgttgc catgcagtac gcagtcattg ccgatgcggc tgttggcgcc
     1441 gatgcacgta cccggaccga cttccacccg ttcacccagc aggcagcccg cctcgatcac
     1501 cacatggggg ccgatccggc aacccgcacc gagtacggca tccggcgcaa tcaccgcagt
     1561 gggatggaca ccgtcaggct gacgggggcg atgattgaac aggtgggagg cacgcgcgta
     1621 agtcagatag ggatttgcca ccaccagaca ggccacggga cattccgccg ccatgtcccc
     1681 agccactatc accgcagagg ccgatgtgga tgccagctgg gcacgataca gcgggttggc
     1741 caggaaactg agctgacctg cctgcgcatc gcccagtgtg gccagacccc gaatgcgaat
     1801 ggctggatcg ccccgcaatt ccgcacccag ttgctgcgcc agctcgccca gcgtcaggcc
     1861 attcccttta ccctgatcag cactcatggc attcattcct ggcagcgatc gttcaatgcc
     1921 gctttacttc tgggcgttga tgcgctgtgt cagttccggg gtgatatcaa atttggggtc
     1981 cacccacagg gcagcctgcc gctgcaggat cagatcgtat ttcttttcat cgacgatggc
     2041 tttcatggct ttttccacct tcggccccat cacggactgc agctcctgac tggcatcctt
     2101 acccttctgc tgcagtttct ggcctttgaa attcagctcg attttcttgt cggcaatttc
     2161 cttttccaga cggcgcaatt cgtcctgccc catcacggcg gaatccttct ggcgcttttc
     2221 atccagcttc tggatctggt tgcgcagggc gatggcatcg gcctcctcct gtcccagagt
     2281 ggacttcagc ttctcggcac gcagacgcgc ctcatccgat ttcatgactg ccgcgttgag
     2341 atcgacaacc gcgatccgga tttccgccca caccggcgcg gaagacaaaa gcagacccgc
     2401 caataccagc aatttgaacc ctttcattaa aaaacccctt gcatgcttgt tgcctgttaa
     2461 atcagaacgg ttgacccaga ctgaactgga acgaccgggt gtcatcgtcg tctttttcgt
     2521 tgagcgtctt cgcca
//
LOCUS       DASUEG010000401         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1619624, whole genome shotgun sequence.
ACCESSION   DASUEG010000401 DASUEG010000000
VERSION     DASUEG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619624"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFX11_07390"
     CDS             <1..237
                     /locus_tag="VFX11_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEX4938481.1"
                     /translation="VQARLGLPASAFSYLFSHGNLDQEHFQFFEKLMNGIDNVEDQRA
                     IVHAANVVYRLYGDMLRSIPLQQAQTVEVNHAAA"
     gene            224..1051
                     /locus_tag="VFX11_07395"
     CDS             224..1051
                     /locus_tag="VFX11_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938482.1"
                     /translation="MQLRDRKVLLTGASGGIGKALAAALLQQGARLVLAGRNLESLQR
                     IARQLLATQGQAKLALAQLDLGSDQMWQQAQAIAQQHPDVDVLVNCAGRNLFAGVAQT
                     DPVQTAAMLDVNLKGTILLTQALLPTLLTRPQAMIVNVGSTFGSIGYPGFAVYCASKF
                     GVRGFTEALQRELAQTRVKVVYVAPRATATDMNEAAVVAMNNELGNKMDAPALVAACV
                     VRAIDRERPRLFIGWPEKLFVFMNGLFTSVVDKALRKQLPVIQRFLSNESKSQEVKS"
     gene            1048..1710
                     /locus_tag="VFX11_07400"
     CDS             1048..1710
                     /locus_tag="VFX11_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938483.1"
                     /translation="MKGSFTTKVVSILATLLCVFAVQAQAAPAFDEQLLTLQEQWAHI
                     NYEVDKNQKEVEFEKLAGQLQQLAAQNPGRPEPLIWRGIVLSTWAGAKGGLGALGLVK
                     DARDLFEQSLKLSPDALQGSAYTSLGSLYYQVPGWPIGFGDDKKARELLEKAIAINPA
                     GIDSNYFYADYWLEQGEYQKAKQYFDKALQAPARPNRPLADAGRRQEIQAKLAQVDKK
                     LH"
     gene            1748..2410
                     /locus_tag="VFX11_07405"
     CDS             1748..2410
                     /locus_tag="VFX11_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938484.1"
                     /translation="MRLLLVEDDAMLGRAVLTGLKMDGYAVDWMSTGQSALHCLETDE
                     YDLVVLDLGLPDIDGKAVLNTLRKRGMTTPVIILTARDSIEDRVGGLDAGADDYMVKP
                     FDQDELMARVRALLRRRSGRASAVLQYKDITLHPDSMKAEYQGQPVEISRREFALLQE
                     LLVNTGRVLTREQLERAIYDWDQDVASNAVEVHIHHLRKKFYNDLIRTVRGVGYLVEK
                     AG"
     gene            2407..>2669
                     /locus_tag="VFX11_07410"
     CDS             2407..>2669
                     /locus_tag="VFX11_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4938485.1"
                     /translation="MKSIRAFLLLSVSLLVLLSLLVSAWLTFDRARYEVDELFDAELA
                     QTARVLRSTVTVANRMTGQPGDAPVPLVSTHDWATRTAEELSS"
BASE COUNT          542 a          737 c          872 g          518 t
ORIGIN      
        1 gtgcaggccc ggctgggtct gccggcgtcg gcattttcct atctgttttc ccacggcaat
       61 ctggatcagg agcatttcca gttctttgaa aagctgatga acggcatcga caacgtcgaa
      121 gaccagcgtg ccatcgtcca cgcggcgaac gtggtgtacc ggctttatgg cgacatgctg
      181 cggtcgattc cgttgcagca ggcccagacc gttgaggtga atcatgcagc tgcgtgacag
      241 gaaggtattg ctgaccggtg ccagtggtgg catcggcaag gcactggcag cggcgctgtt
      301 acagcagggt gcacgcctgg tgctggcagg gcggaatctg gagagcctgc agcggatcgc
      361 acgccagttg ctggccacac agggccaggc gaagctggcg ctggcgcaac tggatctggg
      421 cagtgaccag atgtggcagc aggcgcaggc gattgcacag cagcatccgg atgtggatgt
      481 gctggtgaat tgcgccggtc gcaatctgtt tgccggcgtg gcgcagaccg atccggtgca
      541 gacggcggcc atgctggatg tgaacctgaa aggcaccatc ctgctgaccc aggcgctgct
      601 gcccacgttg ctgacccggc cacaggccat gatagtcaat gtgggttcga cttttggcag
      661 catcggttac cccggctttg ccgtgtactg cgccagcaaa tttggcgtgc gcggattcac
      721 cgaggcgcta caacgtgaac tggcgcagac ccgggtcaag gtggtgtacg tcgcgccccg
      781 cgccactgcc accgacatga acgaggctgc agtggtggcc atgaacaacg agctgggcaa
      841 caaaatggac gcaccggctt tggtggccgc gtgtgtggtg cgggcaatcg accgcgaacg
      901 tccgcgcctt ttcatcggct ggccggaaaa actgttcgtg ttcatgaatg gcctgttcac
      961 cagcgtggtc gacaaggcgt tgcgcaaaca gttgccggtc attcagcgtt ttctttccaa
     1021 cgaatccaaa tcgcaagagg tcaaatcatg aaaggttctt ttacgacaaa ggtggtatcc
     1081 attcttgcca cgctgctgtg tgtcttcgcg gtgcaggcgc aggcagcgcc ggcattcgat
     1141 gagcagctgc tgacgctgca ggagcaatgg gcgcatatca attatgaagt ggataaaaac
     1201 cagaaagagg tcgagttcga aaaactggcg gggcagttgc agcagctggc ggcgcaaaat
     1261 ccgggtcgtc ccgaaccgct gatctggcgc ggtatcgtgc tgtccacctg ggcgggtgcg
     1321 aaaggcggtc tgggtgcgct cggactggtg aaggatgccc gcgacctgtt cgaacagtcg
     1381 ctcaagctgt cgccggatgc tctgcagggt tcggcctata cttcactggg cagcctgtat
     1441 tatcaggtgc cgggctggcc catcggcttc ggtgacgaca agaaggcccg tgaattgctg
     1501 gaaaaggcga tcgcgatcaa tcccgccggt atcgacagca actactttta cgccgattat
     1561 tggctggagc agggcgagta ccagaaggcc aaacagtatt tcgacaaggc cctgcaggcg
     1621 ccggctcgtc cgaaccgccc gctggcggat gccgggcgtc gccaggaaat ccaggccaag
     1681 ctggcccagg tcgacaaaaa actgcactga aaagaaactg caccaagaac aagacaaggc
     1741 tgtgaggatg cgattactgc tggtggaaga cgatgcgatg ctgggacgcg ccgtgctgac
     1801 aggattgaag atggacggct acgcggtgga ctggatgtcc accggacagt cggcactgca
     1861 ttgtctggaa accgacgaat atgatctggt ggtactggat ctcggcttgc cggatatcga
     1921 cggcaaagcc gtgctcaaca ccctgcgcaa acgcggcatg accacgccgg tgatcattct
     1981 cacggcccgc gacagcatcg aggatcgcgt tggcgggctg gatgcgggcg ccgacgatta
     2041 catggtgaag ccgttcgacc aggatgaact gatggcgcgg gtgcgggcgt tactgcggcg
     2101 gcgcagcgga cgcgcctcgg cggtattgca atacaaggac atcacgctgc atcccgattc
     2161 catgaaggcg gaataccagg gtcagccggt cgagatttcg cggcgggaat tcgcgctgct
     2221 gcaggaactg ctggtcaata ccgggcgcgt tctcacccgg gagcagctgg agcgtgcgat
     2281 ttacgactgg gatcaggatg tcgccagcaa cgcggtagag gttcatatcc accatctgcg
     2341 caaaaaattc tataacgacc tgatccgcac tgtgcgcggt gtcggttatc tggtggaaaa
     2401 ggccggatga aatcgatccg cgcctttctg ctgctttcgg tttccctgct ggttttgctc
     2461 tcgttgctgg tgtcggcgtg gctaacgttc gatcgcgccc gttatgaggt ggacgaactg
     2521 ttcgacgccg aactggcgca gaccgcacgg gtgctgcgct ccaccgttac ggtggccaac
     2581 cgcatgacgg gccagccggg tgacgcgccc gtacccctgg tgagcacgca cgattgggcg
     2641 acccgtaccg ccgaggaact gtcaagcga
//
LOCUS       DASUEG010000402         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_601711, whole genome shotgun sequence.
ACCESSION   DASUEG010000402 DASUEG010000000
VERSION     DASUEG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601711"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFX11_07415"
     CDS             <1..569
                     /locus_tag="VFX11_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938486.1"
                     /translation="EYRDDMAWPIINPEGRPVDGACGWAEDFGYLAPPLYGVWASSPY
                     LHNGSVPTLRQVLRSSERPTIWARQDSPVAGKVKGFDMSLNAYDLDENVGWKYREYCS
                     RQDLSPEECNGPDRVRTGLFYRWLDSIKDNFWLAGFISKPYGTQKQIDQRKIYNTHEF
                     SNSNAGHTFTDVLTEAETRAVIEYLKTL"
     gene            575..1072
                     /locus_tag="VFX11_07420"
     CDS             575..1072
                     /locus_tag="VFX11_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4938487.1"
                     /translation="MRFKQYLPLLLALLCGASTGLQAAPAPDFTLPRLDDAAPVSLSA
                     LRGKVVYVDFWASWCAPCTVALPEMDRLRRDLHARGFEVVAINLDESAADALRFIQRM
                     DLSYPVVTDPAKTTPQAYAISGMPTAFLIDRHGELHSTHSGFRPGDSAEIRKTIEQLL
                     QETPP"
     gene            1069..1278
                     /locus_tag="VFX11_07425"
     CDS             1069..1278
                     /locus_tag="VFX11_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEX4938488.1"
                     /translation="MKLLLLACALLLAACSTAPVQPWERGNLARPDMAWDTDPLHSAM
                     RDHVNVSKEGSSGGLNTGGGGCGCY"
     gene            1368..>2048
                     /locus_tag="VFX11_07430"
     CDS             1368..>2048
                     /locus_tag="VFX11_07430"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX4938489.1"
                     /translation="MTITKKCNPLAALTTAALALPAISHLAHADTAPVKTELGYRYSQ
                     YQEDDIDDNKILIGSPQRYEVDNHAFRLVTPLNAQLGLTVDGSYETMSGASAYGIAAD
                     AQGDPKMIMTGASIDDKRTDVSASVRHYREAGSVAFTLGYSGENDYTALNGSIEAEQQ
                     SADRLTTYSGGIGYSSDELEPEQTPGINRPTSEDKSALNAFVAMTRVMNAGWQLQGGL
                     FTGMHDGFR"
BASE COUNT          441 a          663 c          579 g          365 t
ORIGIN      
        1 acgaatatcg cgatgacatg gcatggccca tcatcaatcc ggaaggtcgc ccggtggacg
       61 gagcctgcgg ctgggcagaa gacttcggtt acctggcgcc gccgctgtac ggggtatggg
      121 catcatcacc ctacctgcac aacggatcgg ttcccacttt gcggcaagta ttgcgctcca
      181 gcgaacgccc caccatctgg gcgcggcagg attcgccggt ggccggcaag gtgaaaggtt
      241 tcgacatgag cctgaacgcc tacgacctgg atgaaaacgt cggctggaaa taccgcgaat
      301 attgcagccg gcaggatttg tcgccggaag aatgcaacgg tccggatcgc gtgcgcacag
      361 gtctgtttta tcgctggctc gacagcatca aggacaactt ctggctggcc ggtttcatca
      421 gcaagcccta cggcacccag aagcagatcg accagcgcaa gatttacaac acgcacgaat
      481 tcagcaacag caacgccggc cataccttca ccgacgtgct gactgaagcg gagacccgtg
      541 ccgtgattga atatctgaaa acgctgtgag gatagtgcgc ttcaaacaat acctgcccct
      601 gttgctggca ctgctctgcg gtgccagcac cgggttgcag gcagcccccg cgcccgattt
      661 cacgttgccc cggcttgacg acgcggcacc cgtttcgctg tcggcgctgc ggggcaaagt
      721 ggtgtacgtg gatttctggg catcctggtg cgcaccctgc accgtggccc tgccggaaat
      781 ggaccggctg cgtcgcgatc tgcatgcgcg tggttttgaa gtcgtcgcca tcaatctgga
      841 tgaatccgca gccgacgcgc tgcgttttat ccagcgcatg gatctgtcct acccggtggt
      901 caccgatccg gcaaaaacca cgccgcaagc ttacgccatc agcggcatgc ccaccgcctt
      961 cctgatcgat cgccacggcg aactgcattc cacccacagc ggttttcgcc ccggcgacag
     1021 cgccgaaatc cgcaagacca tcgaacaatt actgcaggag acacccccat gaaactgctg
     1081 ttgctggcct gcgctttact gctggccgcc tgctccaccg cccccgtgca accctgggag
     1141 cgcggcaatc tcgcccgccc cgacatggcc tgggacaccg acccgctcca cagcgccatg
     1201 cgcgatcacg tgaacgtgtc gaaggaaggt tccagcggcg gattgaacac cggcggcggt
     1261 ggctgcggct gctactgatt ccgcgcgcaa aacaacaata atttttaccc gacggccgtt
     1321 ttcaaggccg cattgttaaa gaccgcattg aaggacgcag ttcactcatg acaataacaa
     1381 aaaagtgcaa cccgctggcc gcgctcacca ctgccgcgct ggcactgccc gccatctccc
     1441 atctcgccca cgccgacacc gcgccagtca aaaccgaact gggctatcgc tattcccagt
     1501 atcaggaaga cgacatcgac gacaataaaa tcctgattgg cagtccgcag cgttacgaag
     1561 tggacaatca tgctttccgc ctggtgacgc cgctgaacgc gcaactgggg ctgacggtgg
     1621 atggctcgta cgaaaccatg tccggtgcgt ccgcctacgg cattgccgcc gatgcgcagg
     1681 gcgaccccaa aatgatcatg accggcgcca gcatcgacga caaacgcact gacgtgtccg
     1741 ccagcgtgcg ccattaccgg gaagccggca gcgttgcgtt cacgctgggt tattccggcg
     1801 aaaacgatta caccgccctc aacggcagca tcgaagcaga acagcagtcg gcggatcggc
     1861 tgaccactta ctccggcggc atcggctact ccagcgacga actggaaccg gagcaaacac
     1921 ccggcatcaa tcgccccacg tcggaggaca aatccgcgct caacgcattt gtggccatga
     1981 caagggtgat gaacgccggc tggcaattgc agggcgggct ttttaccggg atgcacgatg
     2041 gcttccgg
//
LOCUS       DASUEG010000403         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_344530, whole genome shotgun sequence.
ACCESSION   DASUEG010000403 DASUEG010000000
VERSION     DASUEG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344530"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFX11_07435"
     CDS             <1..182
                     /locus_tag="VFX11_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX4938490.1"
                     /translation="DNGHVDERTVDVHIYRLRNQLSRFGLGHLIESVRGSGYRLATPK
                     HARRHTVTGQLRFAS"
     gene            211..1050
                     /gene="cysT"
                     /locus_tag="VFX11_07440"
     CDS             211..1050
                     /gene="cysT"
                     /locus_tag="VFX11_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX4938491.1"
                     /translation="MLMLSRTPSVLPGFRLTLGFALFYLSLIVLIPLSGLFFKTATMT
                     WDDFVSTVTAPRVLASYQLSFGASFIAAIINAVFGFLVAWVLVRYRFPGKRIIDALVD
                     LPFALPTAVAGIALTALYAPNGVFGKALEPLGLKVAFTPLGVVVALTFIGLPFVVRTV
                     QPVLEDLELEVEEAAASLGASRWKTFTRIIFPAVLPALITGFALAFARAVGEYGSVIF
                     IAGNMPMISEIAPLMIITKLEQYDYAGATAIATVMLVISFVLLLAINLLQAWTRRQTT
                     AGR"
     gene            1060..1947
                     /gene="cysW"
                     /locus_tag="VFX11_07445"
     CDS             1060..1947
                     /gene="cysW"
                     /locus_tag="VFX11_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX4938492.1"
                     /translation="MSASTRTYTKSVTEPLWIRLLLIGGALLFLAIFLFAPLAAVFGE
                     ALRKGTEAYFESFNDPDALSAIKLTLIAAAIAVPFNLVFGIAAAWAIAKFEFRGKQLL
                     ITLIDLPFAVSPVIAGLIYVLLFGAHGYFGEWLFEHDIKILFAVPGIVLATIFVTFPF
                     VARELIPLMQAQGTEEEEAAITLGASGFRTFLRVTLPNIKWGLLYGVILCNARAMGEF
                     GAVSVVSGHIRGLTNTMPLHVEILYNEYMFVAAFAVASLLALLALVTLALKSFVEWKL
                     AAEMKALRDGFQESAPTQS"
     gene            1961..>2940
                     /locus_tag="VFX11_07450"
     CDS             1961..>2940
                     /locus_tag="VFX11_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX4938493.1"
                     /translation="MSIEIRNVSKRFGDFVALDNVNLNVDSGELVALLGPSGCGKTTL
                     LRIIAGLETADQGSILFHGEDTTDRHVRERQVGFVFQHYALFRHMTVFENVAFGLRVR
                     PRKQRPSEAEIRKKVTTLLELVQLGWLADRYPSQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKDLRRWLRQLHDELHVTSVFVTHDQEEALEVADRIVVLNRGHIEQI
                     GTPDEVYQAPASPFVYSFLGNVNLFHGRVDEGWAEVGGLRLEATEHAGVSADAVAYVR
                     PHDIELSHVELPEAQQAVVDDVRPLGGLVRLVLRHEQSLVEVELSRERYNA"
BASE COUNT          505 a          926 c          864 g          645 t
ORIGIN      
        1 ttgataacgg ccatgtcgac gagcgaacag tggacgtgca catctaccgc ctgcgcaacc
       61 agctgtcacg tttcggactc ggccacctga tcgaatccgt gcgcggcagt ggttaccggc
      121 tcgccactcc gaaacacgca cgccgccata cggttaccgg gcaattgcga tttgcctcct
      181 gacaacactg cccggaatgg aaaacatcac atgcttatgc tgtctcgcac ccccagtgtc
      241 ctgccaggtt ttcgcctgac actgggcttt gccctgttct acctcagcct gatcgtgctg
      301 atcccgctct ccggcctctt tttcaagacg gcaacgatga cttgggacga ctttgtgagc
      361 acggtgaccg caccccgtgt gctggcctcc taccagctca gctttggcgc ctccttcatc
      421 gctgccatca tcaatgccgt cttcggcttt ctggtggcgt gggtactggt gcgttaccgc
      481 tttcccggca agcgcatcat tgacgccctg gtggacctgc cctttgctct gcccaccgct
      541 gtcgccggca ttgccctgac cgcgctttat gcacccaacg gcgtctttgg caaagcgctg
      601 gagcctctgg gcctcaaggt ggctttcacg ccactgggcg tggtggtggc gctgaccttc
      661 atcggcctgc ccttcgtggt gcgcacggtg cagccggtgc tcgaagacct ggaactggaa
      721 gtggaggaag ccgcggccag cctgggcgcc tcccgctgga aaacgttcac ccgcatcatt
      781 ttcccggccg tgctgcccgc cctgattacc ggttttgccc tcgccttcgc acgtgccgtc
      841 ggcgagtacg gttcggtcat cttcatcgcc ggcaacatgc cgatgatttc cgaaattgca
      901 ccgctgatga tcatcaccaa gctggagcag tacgactatg ccggcgccac ggcgattgcc
      961 accgtcatgc tggtgatttc gtttgtactg ttgctcgcca tcaatctgct gcaggcctgg
     1021 acgcgacgcc agaccactgc cggtcgctga ggaaaaatca tgtccgcatc aacccgcacc
     1081 tacacgaaga gcgttaccga accgctttgg atacggctgc tgctgatcgg tggtgccctg
     1141 ctgtttctag ccatcttcct gttcgccccg ttggcggcgg tttttggcga ggccctacgc
     1201 aaaggcaccg aagcctactt cgagtcgttc aatgatccgg atgcgctgtc tgccatcaag
     1261 ctcacgctga tcgccgccgc gattgccgtg ccgttcaatc tcgtgttcgg tatcgccgcg
     1321 gcctgggcca ttgccaaatt cgagttccgc ggcaagcagt tgctgatcac cctgatcgac
     1381 ctgcccttcg ctgtttctcc ggtcattgct ggcctgattt atgtcctgct gtttggcgca
     1441 catggctatt tcggtgagtg gctattcgaa cacgacatca agatcctgtt cgccgtcccc
     1501 ggcatcgtgc tggccaccat cttcgtgacc tttccctttg tggctcgcga actgattccg
     1561 ctgatgcagg cgcagggcac cgaggaagaa gaagcggcca tcacgctggg cgcctccggt
     1621 ttccgcactt ttctgcgcgt cacgctgccc aacatcaaat ggggcctgct ctatggcgtg
     1681 attctctgca acgcacgcgc gatgggcgaa ttcggcgccg tctccgttgt gtccggccac
     1741 attcgcggcc tcaccaacac catgccgctg catgtggaaa ttctctacaa cgaatacatg
     1801 tttgtcgccg cctttgccgt tgcatccctg ctggcattgc tggcgctggt cacattggcc
     1861 ctcaaatcct ttgtggagtg gaagctggcc gccgaaatga aagccctgcg ggacggattc
     1921 caggaatccg caccgacaca gtcgtgagga atgaattgtc atgagcattg aaatcagaaa
     1981 cgtgagcaag cgcttcggcg acttcgtggc actggataac gtcaacctga atgtcgacag
     2041 cggtgagctg gtggcactgc tggggccgtc aggctgcggc aagaccacgc tgttgcgcat
     2101 cattgccgga ctggaaaccg ccgatcaggg cagcatcctg ttccacggcg aggacaccac
     2161 ggaccgccac gtgcgcgaac gtcaggtcgg ttttgtcttc cagcactacg cactgttccg
     2221 gcacatgacg gtgttcgaga acgtggcctt cggcctgcgc gtgcgcccgc gcaagcagcg
     2281 cccgagcgag gccgagatcc gcaagaaggt caccacgctg ctggaactgg tgcagctggg
     2341 ctggctggcc gatcgctacc ccagccagct ctccggcggt cagcgccagc gcattgcgct
     2401 ggcccgcgcc ctggccgtgg agccgaaact gttgctgctg gacgagccct ttggtgcact
     2461 cgacgccaag gtgcgcaagg acttgcgccg ttggttgcgc cagttgcatg acgagctgca
     2521 cgtgacctcc gtgttcgtga cgcatgacca agaggaagca ctggaagtgg cagaccgcat
     2581 tgtggtgctg aatcgcggcc acatcgaaca gatcggcaca ccggacgagg tgtatcaggc
     2641 ccccgcgtca ccgtttgtgt acagcttctt gggcaatgtg aacctgttcc atggccgtgt
     2701 ggatgaaggc tgggccgaag tcggcggcct gcgactggag gctaccgaac acgccggcgt
     2761 cagtgccgac gcagtggcct atgtgcgacc gcatgacatc gagttgtcgc atgtggagct
     2821 gcccgaagca caacaggctg ttgtcgatga cgtgcgtccg ctcggtggtc tggtgcgtct
     2881 ggtcttgcgt cacgagcagt cattggtgga ggtggagctg tcgcgtgagc gctataacgc
//
LOCUS       DASUEG010000404         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_955193, whole genome shotgun sequence.
ACCESSION   DASUEG010000404 DASUEG010000000
VERSION     DASUEG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955193"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1488)
                     /locus_tag="VFX11_07455"
     CDS             complement(130..1488)
                     /locus_tag="VFX11_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX4938494.1"
                     /translation="MHPFRRVSRPFVGGILLAFLSSSALAALPFFDSDGEKIPSLAPM
                     LEKVNPAVVNISVFATRTVQNPLLNDPFFRHFFNIPQHAPQQQQRSQAAGSGVIVDAD
                     KGTVITNYHVIKGADEIKVSLTDGRTLTAKLVGSDPDVDIAVLDVDAKDLTALKMADS
                     EEIRVGDFVVAIGNPFGLGQTVTTGVVSALGRSGLGIEGYENFIQTDASINPGNSGGA
                     LVDLRGQLVGINTAIIAPAGGNVGIGFAIPINMAMNSVNQILDKGEVKRGQLGVVIQD
                     LTPDLAEAFDLKGRQGVVVSEVQKDSAADDAGIEAGDVLIELDGKPLTSAGQLRNQVG
                     LKQIGDKVKVKLLRDGDSRELTVKIGEPSDKVVASEGLHRFLEGASLAASSDPQGVEV
                     KGIEPVSAAEQSGLQEGDVIISANKKRVDSVDSLRKAVAGSDQLLLRVVRGNMALYLV
                     LR"
     gene            complement(1610..2413)
                     /locus_tag="VFX11_07460"
     CDS             complement(1610..2413)
                     /locus_tag="VFX11_07460"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4938495.1"
                     /translation="MSEVLFQCDGDLYVPTALAGSPWHPALLHGGAPAGLLAHCLEQS
                     VGDPRLQPSRLTIDLIRPVPNAPLSVVLRRIRSGKRIVLEEAQLQADGKTVAIATGLF
                     VLPQSVTVPDYAPARQPLAPPHQLQEVSFRDVLFTNSDNMPPGLHTTVRMRPISSLHE
                     SGRGLAWLSLPASIVEGCSNSPFMRAALTSDFSNGVGQLSLGNNVGMINADITLQLFR
                     LPQDEWIGLDATTLVHSGGVGVVTADLHDCHGLCGLVSQTVMPMAEFTR"
     gene            complement(2438..2968)
                     /locus_tag="VFX11_07465"
     CDS             complement(2438..2968)
                     /locus_tag="VFX11_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938496.1"
                     /translation="MAAIDTVSRTISHLARTEFRYADVSVAVLAGRAVARPMMVAAVN
                     DHVRAWVEHILKFVLGAAGAVLILFLLNSPGLPFLGAFSAGLGVLYLANLADVARYRD
                     AIFFIVPTLFIWGMLVLDHHRPELVALTWFTHVFIGFVGSFVRTSGGIAELRLWSLLM
                     GFSLVNLVYLINHVSL"
     gene            3094..>3952
                     /locus_tag="VFX11_07470"
     CDS             3094..>3952
                     /locus_tag="VFX11_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX4938497.1"
                     /translation="MTTELAVLAFDGCFGTSVTGPIDIFNIANIHQRVCREKTASPLF
                     SWKIYSQDGKPVRTSTGVSVAVNGDYSQIGNPDICFVPGIDHAYGNEVVRKINELRGS
                     IGELVKREHAKGATLASVCSGSFVLADTGLLNNREATTSWWLAHHFTEAFPQVNLQLE
                     KIVTEDNNIFCSGAVTAYMHLCIAMIEKFAGSQMAQTCAKTILLQSKSSSQAPYIPVS
                     HHLRQQDDMVQQAVQWMQQNLAQEFQLEDLADKLAVSPRTFIRRFNQATGKPPKQYFQ
                     KLRINEAKRL"
BASE COUNT          880 a         1184 c         1154 g          734 t
ORIGIN      
        1 taggagggat accgggccca taatttgccc ggcatcaaat taaccgcaag gtagacaggc
       61 aattgaaggc gccgagcggg gggcggggta ccggccacgt caacgcgaac gatgtggccg
      121 gtgaacatat caacgcagaa ccagatacag ggccatgttg ccccgcacga cgcgcaacag
      181 cagctggtca ctgccagcga ccgccttgcg cagggaatcc acgctgtcca cccgcttctt
      241 gttggcactg atgatcacat ccccttcctg caggccagac tgctcggccg cagagacagg
      301 ctcgatgcct ttcacctcca caccctgggg atcgctgctg gccgcaagac tggcaccttc
      361 cagaaaacgg tgcagacctt ctgaggccac caccttgtcg ctgggctcgc cgatcttcac
      421 tgtcaattcg cgggagtcac cgtcccgcag cagcttgacc ttcaccttgt ccccgatctg
      481 cttcagaccc acctggttac gcagctgacc ggcgctggtg agtggcttgc catccagctc
      541 gatcagcacg tcgcctgcct cgataccggc atcatccgcc gccgagtcct tctgcacttc
      601 cgataccacc acgccctgcc tgcctttcag gtcgaaggct tccgccagat ccggggtcag
      661 atcctggatc actacgccca gctggccgcg cttcacttca cccttgtcca gaatctggtt
      721 gacgctgttc atggccatgt tgatggggat ggcgaaacca atgccgacat tgccgccggc
      781 cggcgcgata atggcggtgt tgatgcccac cagctgaccc ctgagatcca cgagcgctcc
      841 cccggaatta ccagggttga tggatgcatc cgtctggatg aagttttcgt agccttcaat
      901 gccgaggccg ctgcggccca gggcactcac cacgcccgtc gtgacggtct ggcccaggcc
      961 gaacgggtta ccgatcgcca caacgaaatc tccgacccgg atttcctccg aatccgccat
     1021 cttcagtgcg gtcagatcct tggcgtccac atccagcacg gcgatgtcca cgtccggatc
     1081 ggagcctacc agtttggcgg tcagcgttct gccatcggtg agtgacacct tgatttcatc
     1141 ggcgcccttg atgacgtggt aattggtaat caccgtgccc ttgtctgcat ccacgatcac
     1201 gccggaaccg gctgcctggg agcgctgctg ctgttggggt gcatgctggg gaatattgaa
     1261 gaaatgccgg aagaagggat cgttcagcaa cggattctgc accgtgcggg tcgcgaatac
     1321 ggaaatgttt accaccgcgg gattgacctt ttccagcatg ggcgccagcg acggaatttt
     1381 ttccccatca ctgtcaaaga aaggcagcgc tgccagggcc gaactgctca ggaatgccaa
     1441 caggatcccg ccaacaaacg gccgggacac gcgccggaat gggtgcatga ctacctccaa
     1501 tgtgaatgct gaaacgcttg ggaaaatggg gctatgttgc catattttca actggcgatt
     1561 cgactcgatc caggagggga aagttccggt ggccgtctgt cgggcgtttt cagcgggtga
     1621 attcagccat gggcatcacg gtttgggaaa ccaggccaca cagcccgtga cagtcatgca
     1681 ggtctgccgt gacgacaccc actccgccgg aatgcaccag cgtggtagcg tcaagtccga
     1741 tccattcgtc ctgcggcagg cggaacaatt gcagggtgat gtcagcattg atcatgccca
     1801 cgttgttgcc caggctgagc tgaccgacac cgttgctgaa atctgaggtc agggcggccc
     1861 gcatgaaagg agaattggaa cagccttcca caatggaagc gggcagcgac agccaggcca
     1921 gaccgcgccc gctttcgtgg agtgaagaga tgggccgcat gcgaacggtg gtatgcaggc
     1981 cgggcggcat gttgtcactg ttggtgaaca gcacgtcgcg aaaactgact tcctgcagct
     2041 gatgcggcgg cgccaacggc tgtcgcgcgg gtgcgtaatc cggcaccgtc actgactgcg
     2101 gcaatacaaa caggccggtg gcgatggcga cggtcttgcc gtcggcctgc agctgggctt
     2161 cttccaatac gatgcgcttg cctgatctga tgcggcgcaa taccacgctg agcggcgcat
     2221 tcggcaccgg gcggatcagg tcaatggtga gcctggaagg ttgcagccgg gggtcgccaa
     2281 cgctctgctc caggcaatgc gccagcaatc ccgccggcgc gccgccgtgc agcagcgccg
     2341 gatgccaggg actgccggcc aaggccgtgg gcacatacag gtcaccatca cactgaaaca
     2401 acacttcgct catgatgcaa gaccatcctc ttccaaatca caggctcaca tggttgatca
     2461 ggtacaccag attcaccagc gagaatccca tcagcagcga ccagaggcgc agttcggcaa
     2521 tgccaccgct ggtacggacg aaagaaccaa cgaaaccgat gaacacatgg gtgaaccagg
     2581 tcagcgccac cagttccggc cggtgatgat cgagcaccag catgccccag ataaacaggg
     2641 tgggcacgat gaagaagatg gcatcgcgat accgggccac atctgccaga ttggccaggt
     2701 acaacacacc cagacccgca ctgaaggcac ccagaaacgg cagacccgga ctgttcagca
     2761 ggaacagaat caggaccgcg ccggcagcgc ccagcacaaa cttgaggatg tgctccaccc
     2821 aggcacgaac atgatcattg acggcggcga ccatcatggg acgggcaaca gcgcggccgg
     2881 ccagcacagc gacagacaca tcggcataac ggaactcggt gcgggcgaga tgggaaatgg
     2941 tgcgggatac agtatcgatg gcagccatgg cgaaaccctc ctgtgttggt aatcgcgtgt
     3001 tgctacgtgt ccagattagc ccttgacggc aaacagacaa agtggcatta atgacaatat
     3061 tgatgctctt tttgccaccc aaaggaagca attatgacaa ctgaactggc agttttggca
     3121 tttgacggat gttttggaac tagcgtcacc gggccgatcg atatcttcaa catcgccaat
     3181 atccaccagc gggtgtgccg cgaaaagacc gctagccccc tgttttcctg gaaaatctac
     3241 tcccaggatg gcaagccggt gcgaacctca accggggtca gcgtggcggt aaatggcgac
     3301 tactcccaga ttggcaatcc tgacatctgt ttcgtgccgg gaattgacca tgcctatggc
     3361 aatgaagtgg tccggaagat aaatgaactg cgcggcagca ttggcgagct ggtgaaacgg
     3421 gagcatgcga agggtgcgac cctggcatcc gtgtgcagcg gttcctttgt gctggcagac
     3481 acgggcctgc tgaacaatcg cgaggccact accagctggt ggctggcgca tcatttcacc
     3541 gaagcgtttc cgcaggtgaa tctgcaactg gaaaaaatcg tcaccgagga caacaacatt
     3601 ttctgctccg gcgccgtgac cgcgtacatg catctgtgca tcgcgatgat cgagaaattt
     3661 gccggcagcc agatggccca gacctgcgcg aaaaccattt tgctgcagag caagtcgtcg
     3721 tcgcaggccc cgtatattcc ggtatcgcat cacctgcgcc agcaggacga catggtgcaa
     3781 caggcggtgc agtggatgca gcagaatctg gcacaggaat tccagctgga ggatctggcg
     3841 gacaagctgg cagtgagccc gcggacgttt atccggcgct tcaaccaggc taccggcaag
     3901 cctcccaagc agtatttcca gaaactgcgg atcaacgaag ccaagcgatt gc
//
LOCUS       DASUEG010000405         8599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1616262, whole genome shotgun sequence.
ACCESSION   DASUEG010000405 DASUEG010000000
VERSION     DASUEG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616262"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..2133
                     /locus_tag="VFX11_07475"
     CDS             532..2133
                     /locus_tag="VFX11_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX4938498.1"
                     /translation="MTSSTKTTAAFAELSPEQRQALFDKLRQRKLTNARRPAAETVDH
                     SGDTLALGPAQTALLPLLTETTQNRILELFIEGPLCLTALRAAVALLSDRHNGLRVRF
                     DSERKAFASLDQPLSLRTFASTADTAADLQAACTQLAGDPAQALQLACLHAEENVSHL
                     LLASHPLLLDSGSLLFLASQVLALASGNATPDSIHATDSALLNRYTQWSAQVLEQKFL
                     AQEWARLKPRSLDSVARNKPLAGVLPQQQSLFLDNDFIQARISGGESIKNWMGDAIHR
                     CLQGWLSHQEILYWFSAPQLRDEQFETLPGFFPYFVPVTSNEREPLAFNQRLNRLHTR
                     FSPVSEQLSLDLCTQGSSAPMVHYHWFEAGTGDASPIQVRRVIHHQPGILLSPFEIQI
                     TEMLDGINLDVHFDPTHIGTDQVNALLRDLMAYLREEKETEGANRPTLHERLGQIWKD
                     LLQKTEIGNDQSFFELGGHSLQVTELKFRIKQQLKLDVPISVLYELTTINKLANFILA
                     THATGLGWNGATAANAEEDEEEGVL"
     gene            2278..2631
                     /locus_tag="VFX11_07480"
     CDS             2278..2631
                     /locus_tag="VFX11_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX4938499.1"
                     /translation="MNHTLITILLLVCSNVFMTFAWYAHLKNLADKPWIIAALVSWGI
                     ALFEYLLQVPANRIGHEVMNVAQLKILQEVITLSVFVPFAIFYMKEKITLDFLWAGLC
                     LLGAVFFMFRQKLFA"
     gene            complement(2693..3121)
                     /locus_tag="VFX11_07485"
     CDS             complement(2693..3121)
                     /locus_tag="VFX11_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938500.1"
                     /translation="MLRLEFNKLAWWYWLVADVLLMLALWVERDCFVVALAFNLFQLL
                     HFSLRQRSFTAFPVQVRAGYWLWLLAGLAPGLFWMHWIQLAGTSAMLLFGYCPLARML
                     SLLPWNRRQPFTLQFMVRTFLAPPTSGSIRDNPHLVTPPT"
     gene            complement(3135..4088)
                     /locus_tag="VFX11_07490"
     CDS             complement(3135..4088)
                     /locus_tag="VFX11_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938501.1"
                     /translation="MGIQFQPDTARYARCVENSRRVRWDIDRDVIRGRRFESHQKFLP
                     DGLTRVDEFPTRAAAEIRLISQIQGRTYANIFGLVERFINAKVLEVSQHHWMGDQIAL
                     EALVRFSEEELKHQELFRRIEGLCGDVMPAGYHFVPEPNAVAAAVLSKSTWAVLALTC
                     HIELFTQAHFRESIAPDTALSDLYRDVFRYHWQEEAQHAVLDEIEWKREDMALNPAQR
                     EQAVDDFIALVGAVDGILQQQAQADCDYFAAVCGRRLDAPTLERFYIAFLLAYRWQYI
                     FSGVQVQRFMDVLTSMITDAQRDRISTALQGLIVPFELMQA"
     gene            complement(4244..6325)
                     /locus_tag="VFX11_07495"
     CDS             complement(4244..6325)
                     /locus_tag="VFX11_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4938502.1"
                     /translation="MPDTLLQASFPFHLDSGFGQILQNTSAIVYVRDRQGRFIFANRR
                     FLNQFGTSAPALLGKTCADLFPPDIARQYEANDARVLQQRQVMEFEEIAQVDGVEHTY
                     ISIKFPLFDAAGEVLALCGISTDITVRKQRENLLYDVAIKLCSIPDESLFDDVVRYLG
                     PRLGVDFVMIGRLRDGAPDQVSAVAACEQGQLITDLSYDLLGTPCAYVVGREFYCVPR
                     GLQQLFPGDNMLSSAGYESYAGYPLYDSADRPLGLLAVTHRAPLEDIPFVESILKIFS
                     LRVAAALERRIAEQEKRAAEDERRRLSLQLKQAQKMEAIGQLTGGIAHDFNNLLTGVM
                     GYISLAQERAEKLQDAKLDQYLERARSAGRKAGELIQQMLTFSRGEHGVPRVVELHQR
                     LPEFVSLIESSLPAGVELELQLPAALPLVRIDPVHLEQVLMNLCINARDAMESRGRLT
                     LSAASLQLDDRVCESCQQAIAGDYVAIRVQDTGHGILPAVMERMFEPFYSTKEIGKGS
                     GMGLAMVHGILHECGGHVLVESHVGAGSTFTLLLPSAAAAVDNSAQSAALAAPRQQYL
                     QGRVLVVDDQPVVSEFMQDLLANWGLDALVFNHPLQALERMTQQPDAVCAAIIDYTMP
                     KMTGLELAAALARLRPQLPLILYSGYREEIDLAAAQKLGVLDVLRKPIDEQRLLHLLL
                     PLVETMATAQK"
     gene            complement(6438..7166)
                     /locus_tag="VFX11_07500"
     CDS             complement(6438..7166)
                     /locus_tag="VFX11_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938503.1"
                     /translation="MQETVRILVVDDQVDVRRFLEDYLRDKGFATVALGSGAELRSWL
                     ARQVPELVLLDVGLPGEDGLSLARHIREQYDIGIIMVSGAGETLDRVVGLEVGADDYL
                     AKPFEPRELLARVKSVLRRYQRAPVDASGRKQAAMRQVRFGNCLLDLDRRQLLDGNGD
                     DIPVTAMEFDLLQVFAERPHRPLSRDQLMDLTQHRDWDPNDRSIDIRITRLRKKIEPD
                     PDKPSVIRTIRGVGYLYSPADADN"
     gene            complement(7248..7976)
                     /locus_tag="VFX11_07505"
     CDS             complement(7248..7976)
                     /locus_tag="VFX11_07505"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938504.1"
                     /translation="MFDDKADTREKLILVAIRMFAEQGFAGVSMRTINSAAGTRNSSA
                     VHYHFGNKAGIIQAIFDKLEAELRPLFDRLVEELKIRRAEQKLVVEDVVMALQLPFWV
                     LYNTPGYGKHAVKLLARLIQEADGEMQDLYNRYIQEPAAGLFELLQGLMPNKREQQLK
                     FQLVHCLMSTISGMATMNQLSRTPLGDIRFEQDLEMLLSYVQYVSHGLGCESTDMQFD
                     MGFWGRYTEYLMPGQMSAEISQAG"
     gene            complement(8124..>8599)
                     /locus_tag="VFX11_07510"
     CDS             complement(8124..>8599)
                     /locus_tag="VFX11_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX4938505.1"
                     /translation="VDLMIGTSAKPRHRRDLLLPPEAVRERLQEKHGALGDCALVFGR
                     EESGLHGTEIALCDLLSSIPLAVTYPSLNLGQAVMLYAYVLQQLQRENTVEAPAPDQY
                     AALRSKCEILLRDTGYLPGEALFQWALDRLPLFNRRDIGFAHHLIDKLSGRNRRG"
BASE COUNT         1768 a         2722 c         2491 g         1618 t
ORIGIN      
        1 aacgtgcggg aaccgagcgc ggggccgtcg gcgcctttcg ccaccccgtt cattccggag
       61 gactacccgc aagcgggacg gtccttgact gccgaggcgc tctggcactg gtaaacatcg
      121 ttgccataaa cataggctga gcaaaaaaca atctgccata ctggtctgcc aggacatctg
      181 cacgctgaaa tgatgcctac ttacaaactg caacttaacg tgacaaaact cctaaacaaa
      241 tcaagccgtt ttggataccc taatgtccac cttatacaag tcttgtgtga gccgttggga
      301 aataatagcg ctgccagctc tacctgctag gggccacggg attacggtca tattgggccg
      361 ttcccgaaag tggatagacg ctgggcaagg ctgccttgaa gcccgcacgg gctggcagtc
      421 gacgcaggga gcgggggacc atgtttccgg cacggacgcc gaagaccgcc acatccggga
      481 attgacaccg ggtgtaactc agacaagaaa aaaaacaaga gcgcttcgcc catgacgtcg
      541 tccaccaaaa ccactgctgc ctttgccgaa ctcagccccg aacagcgcca ggccctgttc
      601 gacaagctgc ggcagcgcaa gctcaccaat gcccgtcgcc ccgcggcaga aacagtcgat
      661 cactcaggtg acaccctggc gctgggcccg gcccagactg ccctgctccc gctgctgacc
      721 gaaaccaccc agaaccggat tctggaactg tttatcgaag gccctctgtg cctgaccgca
      781 ctgcgtgccg cggtcgcctt gctgagcgac cggcacaacg gactgcgggt acgcttcgac
      841 agcgaacgca aggccttcgc cagcctcgac cagccgcttt cgctgcgcac ctttgcttcc
      901 accgctgaca cggccgccga cctgcaagca gcctgcaccc agctggccgg cgatccggcc
      961 caggcactgc aactggcctg cctgcacgct gaggaaaacg tcagccacct gttactggcc
     1021 agtcacccgt tgctgctgga ttccggttcc ctgctgttcc tggccagcca ggtgctggca
     1081 ctggcttccg gcaatgccac gccggattcg atccacgcca ccgacagcgc tctgctgaac
     1141 cgctataccc agtggtccgc ccaggtactg gaacagaaat tcctggccca ggaatgggcg
     1201 cgcctcaagc cccgctctct ggacagcgtg gcccgcaaca agccgctggc cggcgtgctg
     1261 ccgcagcagc agtccctgtt cctcgacaac gatttcatcc aggcgcgcat cagcggtggc
     1321 gagagcatca agaactggat gggggatgcc atccatcgct gcctgcaggg ctggctgtcg
     1381 caccaggaaa tcctgtactg gttcagcgcc ccccagctgc gtgacgagca attcgaaacc
     1441 ctgccgggct ttttccccta ttttgtgccg gtcaccagca atgaacgcga accgcttgcc
     1501 ttcaatcagc gcctgaaccg cctgcacacc cgcttttcac ccgtgtcgga gcagttgagc
     1561 ctggatctgt gcacccaggg cagcagtgcg cccatggttc attaccactg gttcgaggcc
     1621 ggcaccggtg acgccagtcc gatccaggtg cgccgcgtga ttcatcacca gccgggcatt
     1681 ctgctgtcgc cgttcgaaat ccagatcacc gaaatgctgg acggcatcaa cctggatgtg
     1741 cacttcgatc ccacccacat cggcaccgat caggtgaatg cgctgttgcg ggatctgatg
     1801 gcgtacctgc gcgaggaaaa agaaaccgaa ggtgccaacc ggcccaccct gcacgaacgc
     1861 ctgggccaga tctggaaaga cctgctgcag aaaaccgaga ttggcaacga ccagagtttt
     1921 tttgagctgg gcggccacag cctgcaggtg accgaactga aattccgcat caagcagcag
     1981 ctgaaactgg atgtgccgat ttcagtgctg tacgaactca ccaccatcaa caagctggca
     2041 aacttcatcc tggccacgca tgccaccggc ctgggctgga acggcgcgac ggctgccaac
     2101 gcggaggaag acgaggagga aggcgtgctg taaacacgtc ttccctgaat atcacagccc
     2161 gactgcgcct ggacatcgcc agtaacaagg caccgtcgcg ctggcgttgc acttgcccac
     2221 gcccgcacgg ctcccgtata actggcgctt ccgttggcca tccggttgtc gcaccccatg
     2281 aaccacaccc tgatcaccat tctgctgctg gtctgcagca acgtcttcat gacgttcgcc
     2341 tggtatgccc acctgaaaaa cctggcagac aagccctgga tcatcgccgc gctggtcagc
     2401 tggggcatcg ccctgttcga atacctgctg caggtgcccg ccaaccgcat cggccatgaa
     2461 gtgatgaacg tcgcgcagct gaaaatcctg caggaagtga tcaccctcag cgtgttcgtg
     2521 ccattcgcga ttttctacat gaaagaaaaa atcaccctgg atttcctgtg ggccggattg
     2581 tgtctgctgg gcgcagtgtt tttcatgttc cggcaaaaac tgtttgcatg aaccggcacc
     2641 gaaccgcgcc tgcgccggcc tggccgcaga cggatgcccg ttgctcggga catcaggttg
     2701 gcggagtcac cagatgggga ttgtcccgga tggaaccgct ggtgggcggt gccagaaatg
     2761 tgcgcaccat gaattgcagc gtgaacggtt gtcgccggtt ccagggtagc agcgacagca
     2821 tgcgcgccag cgggcagtag ccaaacagca gcatggcgct ggtgccggcc agctggatcc
     2881 agtgcatcca gaacagaccg ggcgccagcc ccgccagcaa ccacaaccag taaccggcac
     2941 gcacctgcac gggaaacgcg gtgaatgaac gttgccgcag actgaagtgc aacaactgga
     3001 acagattgaa cgccagcgcg acgacaaaac aatcccgctc cacccacagc gccagcatca
     3061 gcagtacatc tgccaccagc cagtaccacc aggccagttt gttgaattca agccgcagca
     3121 tacgttgtcc tccctcaagc ctgcatcagt tcgaaaggca cgatcaatcc ctgcagcgca
     3181 gtgctgatgc gatcacgttg tgcgtcggta atcatgctgg tgagcacatc catgaaacgc
     3241 tgcacctgca cgccggaaaa aatgtactgc cagcggtatg ccagcaggaa ggcgatataa
     3301 aagcgctcca gcgtcggcgc atccagcctg cggccacaca ccgcggcaaa ataatcgcag
     3361 tcggcctgcg cctgttgctg caggatgccg tccaccgcgc ccaccagcgc gatgaaatca
     3421 tcgaccgcct gttcccgctg cgccggattc agcgccatgt cctcgcgctt ccattcgatt
     3481 tcatccagca ccgcatgctg ggcctcttcc tgccagtgat agcgaaatac atcgcgatac
     3541 agatccgaca gcgcggtatc cggcgcgatg ctttccctga aatgcgcctg agtaaacagt
     3601 tcgatgtgac aggtcagcgc cagcactgcc caggtgcttt tcgacagcac ggcagcagcg
     3661 acggcattgg gctccggcac gaagtggtaa ccggcgggca tcacgtcacc acacaggcct
     3721 tcaatgcggc ggaacagctc ctgatgcttg agttcctcct cactgaatcg caccagcgct
     3781 tccagtgcaa tctgatcgcc catccagtgg tgctgactca cttccagcac cttggcgttg
     3841 atgaagcgtt ccaccaaccc gaagatattg gcgtaggtgc ggccctggat ctggctgatc
     3901 agacggatct cggcagcagc acgggtggga aattcgtcca cgcgggtcag gccatccggc
     3961 agaaatttct ggtgggattc aaaacggcgg ccgcgaatga cgtcgcggtc gatgtcccag
     4021 cgtacgcggc gggagttttc cacgcagcgg gcatagcggg cagtatcagg ctggaattgg
     4081 attcccatga tgaagctcct tgtcgtcaat aaaagagaat ggtcgtgtga cggactgtca
     4141 tgaagacggg ctggcgtcgt ggctggccat cgtcgagaca aggagaagat tacgcccgcc
     4201 ctgtatcggc ggtttttctg cgatgttgaa aattgttggt gaattatttc tgcgctgtcg
     4261 ccattgtttc caccagtggc aacagcaggt gcagcaggcg ttgttcatcg atgggtttgc
     4321 gcaacacgtc gagtacgcca agtttttgcg ccgctgccag atcaatttct tcccggtagc
     4381 ccgaatagag aatcagcggc aattgcggcc gcagccgtgc cagcgccgcc gccagctcca
     4441 gcccggtcat tttcggcatg gtgtaatcga tgatggcggc acacacggca tccggttgct
     4501 gcgtcatgcg ttccagcgcc tgcagcggat ggttgaatac cagcgcgtcc aggccccagt
     4561 ttgccagcag atcctgcatg aactcactca ccaccggctg atcatccacc accaaaacgc
     4621 ggccctgcag gtactgttgc cgtggcgcgg ctaaagcagc ggactgcgcg ctgttgtcca
     4681 ccgcggcggc ggccgatggc aacagcagcg taaacgtgct gcccgcgccc acgtgcgact
     4741 ccaccagcac gtgaccaccg cactcatgca gaatgccgtg caccatggcc agtcccatgc
     4801 cactgccctt gccgatttcc ttggtggaat agaacggctc gaacatgcgc tccatcacgg
     4861 cgggcaaaat gccatgaccg gtgtcctgca cacggatggc aacatagtcg ccggcaatgg
     4921 cctgctgaca ggattcgcag acgcggtcat ccagctgcag cgacgccgcg ctcagtgtca
     4981 gtcggccacg gctttccatg gcgtcgcggg cgttgatgca cagattcatc aacacctgtt
     5041 ccaggtgcac cggatcgatc cgcacaagcg gcaatgctgc aggcagctgc aattccagtt
     5101 ccacgccagc cggcaggctc gattcgatca ggctgacaaa ttccggcaac cgctggtgca
     5161 attccaccac ccgtggcacc ccgtgttcac cacgactgaa cgtgagcatt tgctggatca
     5221 gctcaccggc cttgcgtccg gcagaacggg cgcgctccag atactggtcc agtttcgcgt
     5281 cctgcaattt ttcggcacgc tcctgcgcca gactgatgta ccccatcacg ccggtgagca
     5341 gattattgaa gtcatgggcg ataccgccgg tgagctggcc aatggcctcc attttctgcg
     5401 cctgtttcag ctgcagggac agacgcctgc gctcgtcctc tgctgcacgc ttttcctgtt
     5461 ccgcaatgcg ccgttccagc gccgccgcca cgcgcaggga aaatattttc aggatcgatt
     5521 caacaaacgg aatgtcctcc agcggcgcgc ggtgtgtcac ggccagcaaa cccagcggac
     5581 ggtcggctga atcatacagc gggtaaccgg cgtaactttc atagccggcg gagctgagca
     5641 tgttgtcacc gggaaacagc tgttgcaggc ctcgtggcac gcaatagaat tcgcgcccca
     5701 ccacgtaggc gcagggtgtg ccgagcaggt cataactgag atcggtgatc agctggccct
     5761 gttcgcaggc tgctaccgcg ctcacctgat ccggcgcacc atcccgcagg cggccgatca
     5821 tgacgaaatc cacccccagt cgcggaccca gatatcgcac cacgtcatcg aacagggatt
     5881 catcgggaat cgagcagagt tttatggcca cgtcatacag cagattttcc cgctgcttgc
     5941 gcacggtgat gtcggtggaa ataccgcaca gggccagcac ctcgcccgca gcatcgaaca
     6001 gcggaaactt gatggaaatg taggtgtgct cgacaccatc cacctgcgcg atttcttcaa
     6061 attccatcac ctgtcgctgc tgcagcaccc gggcgtcatt ggcttcgtac tggcgggcga
     6121 tgtcaggcgg aaacaggtcg gcgcaggttt tacccagcag cgcgggcgca ctggtaccga
     6181 actggttcag gaagcggcgg ttggcaaaaa tgaaacggcc ctggcgatcg cgcacgtaca
     6241 caatggcgga tgtattctgc aggatctggc caaagccgga atcaaggtgg aacggaaagg
     6301 acgcctgaag gagggtatcc ggcatggcag cattctcgtg cagcggggca ttgacaccat
     6361 tctagaacga atagcacggc ggcacggcgc ggtggaaaca attgaaacag ttgaaacaaa
     6421 gggcgcgaaa gcgcgcttca gttatccgcg tccgccgggc tgtacaggta acccactccg
     6481 cgtatcgtcc tgatcacact gggcttgtcc gggtccggtt cgattttctt gcgcaggcgg
     6541 gtgatgcgga tatcgatgct gcggtcgttg gggtcccagt cgcggtgctg ggtcaggtcc
     6601 atcagctggt cgcggctgag ggggcgatgg gggcgttcgg cgaacacctg cagcagatcg
     6661 aattccatgg cggtgacggg aatgtcgtcg ccgttgccat ccagcagctg acgccggtcc
     6721 aggtccagca ggcagttgcc gaaacgcacc tggcgcatgg cggcctgctt ccgcccggag
     6781 gcgtcaaccg gcgcgcgctg atagcgccgc agcacactct tcacccgcgc cagcagttcg
     6841 cgcggttcaa acggcttggc gaggtaatcg tcggcgccaa cttccagacc caccacccgg
     6901 tccagggttt cgccggcacc ggacaccatg atgatgccga tatcgtactg ttcgcggata
     6961 tgacgggcca ggctcagacc gtcctcaccg ggcaggccca catccagcaa caccagctcg
     7021 ggcacttgcc gcgccagcca agaccgcagt tcggcgccgg agcccagcgc aaccgtggca
     7081 aaccccttgt cgcgcaggta atcctccaga aaccgacgca cgtcgacctg atcgtccact
     7141 accagaatcc tgacggtttc ctgcatggct ggcttcctgc acggatgatg gcctgctggc
     7201 acgctgggca ggcggttggc gatgcgggcg cgaactccgc cgacgtgtca gccggcctgg
     7261 ctgatctcgg cggacatctg ccccggcatc aggtattcgg tatagcggcc ccagaatccc
     7321 atgtcgaact gcatatcggt ggattcgcac cccaggccgt gggacacgta ctgcacatag
     7381 ctgagcagca tttccagatc ctgttcgaag cggatgtcgc ccagcggcgt gcgtgacagc
     7441 tggttcatgg tggccatgcc ggagatggtg gacatcaggc aatgcaccag ctggaatttc
     7501 agttgctgct cgcgcttgtt cggcatcagg ccctgcagca attcgaacaa gcccgccgcc
     7561 ggttcctgga tatagcgatt gtagagatcc tgcatctcgc catccgcttc ctggatcagg
     7621 cgggccagca gcttgaccgc gtgcttgccg taaccgggcg tgttgtagag cacccagaat
     7681 ggcagctgca gcgccatcac cacgtcttcc actaccagtt tctgctccgc acggcgtatc
     7741 ttcagttcct ccaccaagcg gtcgaacagg ggccgcagtt cggcttccag cttgtcgaaa
     7801 attgcctgga tgatcccggc cttgttgccg aagtggtaat gcaccgccga ggaattgcgg
     7861 gtaccggcag cgctgttgat cgtgcgcatg gaaacccccg caaacccctg ttccgcaaac
     7921 atcctgatcg ccaccaggat cagcttttcc cgggtatcgg ccttgtcgtc aaacacggtt
     7981 ctttcctcta cagcatgatt cgtgggcatg ggattcgggc attctgtggc aggggttcag
     8041 gcccgcatta tagccagttg aaaccggtga cacacagtga aaactttgtc cgggagggga
     8101 gcctgggagg ggccggaggc acatcacccc ctgcggttgc ggccgctcag cttgtcgatc
     8161 aggtggtggg cgaatccaat gtcacgtcgg ttgaacagcg gcaggcggtc cagtgcccac
     8221 tggaacaggg cttctcccgg caggtagccg gtgtcccgca gcagaatctc acatttgctg
     8281 cgcagggcgg cgtactggtc cggcgcaggc gcctctaccg tgttttcccg ctgcagctgc
     8341 tgcaacacat aggcgtacag catcaccgcc tgccccagat tgagcgaggg ataggtcacc
     8401 gccagcggaa tcgaactgag cagatcgcag agcgcgattt ccgttccgtg cagaccggac
     8461 tcctcacggc caaacaccag ggcgcaatcc cccagtgcac cgtgtttttc ctgcaggcgt
     8521 tcgcgcaccg cttccggcgg caataacagg tcgcggcggt gacggggctt ggcggaagtg
     8581 ccgatcatca ggtccactt
//
LOCUS       DASUEG010000406         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1458547, whole genome shotgun sequence.
ACCESSION   DASUEG010000406 DASUEG010000000
VERSION     DASUEG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458547"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="ruvB"
                     /locus_tag="VFX11_07515"
     CDS             <1..813
                     /gene="ruvB"
                     /locus_tag="VFX11_07515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX4938506.1"
                     /translation="EMGGNLKSTSGPVLEKAGDLAALLTNLEEGDVLFVDEIHRLSPV
                     VEEILYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLVGATTRAGLLTSPLRDRFGIVQ
                     RLEFYSVEELSRIVSRSAAILGVAMDAEGATEIARRSRGTPRIANRLLRRVRDYAEVK
                     GDGRVTIETAGKALDMLKVDSHGFDTMDRRLLLTIMEKFDGGPVGVESLAAAISEEAG
                     TLEDVVEPFLIQQGFMMRTPRGRVVTRQAWLHFGLNPPARTTTATQDLFTNE"
     gene            806..1231
                     /gene="ybgC"
                     /locus_tag="VFX11_07520"
     CDS             806..1231
                     /gene="ybgC"
                     /locus_tag="VFX11_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX4938507.1"
                     /translation="MNEFSISLRVYLEDTDAGGIVYYVNYLKFMERARTELLRSLGFE
                     FAALSAEQLQFVVHSVDAQYRKPARMDDELIITAVVEQLARTYIVFHQQVRRGDEILC
                     EAHIKVACVDTAQLRPAALPAPVKLQFSQARLPPGPNSH"
     gene            1259..1939
                     /gene="tolQ"
                     /locus_tag="VFX11_07525"
     CDS             1259..1939
                     /gene="tolQ"
                     /locus_tag="VFX11_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX4938508.1"
                     /translation="MSILSLIANATIPVKIVMLILLSLSVASWYMIWQRQTVMSKTQK
                     AQTSFEERFWSGIDLSRLFVQVNTDPNPHCGQENIFRAGFKEFARLRKNLNSDPDSVM
                     EGTERAMRVALLREQEKLETHLPFLATVGSTSPYIGLFGTVWGIMHSFLNLANQQQAT
                     LAAVAPGIAEALIATAIGLFAAIPAVVGYNRFSAVVDRLLHSYETFSDEFSSILHRKA
                     HSGEADDK"
     gene            1963..2403
                     /gene="tolR"
                     /locus_tag="VFX11_07530"
     CDS             1963..2403
                     /gene="tolR"
                     /locus_tag="VFX11_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX4938509.1"
                     /translation="MAQKKTTVRRKPMSEINVVPYIDVMLVLLVVFMITAPMMTAGVK
                     VDLPDADAKPIEMPKDEEFVIVSVNQAGQYYLSVGGDTKEPRPMEEVADMVGKIKRNK
                     PATPVLMEGDHHAAYGLVVQMMVALQKVGVNDVGLVTESPHKEP"
     gene            2406..>2714
                     /locus_tag="VFX11_07535"
     CDS             2406..>2714
                     /locus_tag="VFX11_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938510.1"
                     /translation="MVSQRLDLAALSKSLLVHALVIGMFFVSWKSTDEVVTAKAMPMH
                     ITAMVMEKPQPKTAPKPQPKPAAQPKPPEPKPEPKKPEPKKPEPKKPEPKKPEIKKPE
                     P"
BASE COUNT          570 a          817 c          803 g          524 t
ORIGIN      
        1 gaaatgggcg gcaatctgaa aagcacctcg ggcccggtac tggagaaggc cggcgatctg
       61 gctgcgctgc tgaccaatct ggaagagggc gacgtgctgt ttgtggatga gatccaccga
      121 ctcagccccg tggtcgagga aattctttac cctgccatgg aagactacca gctcgacatc
      181 atgattggcg aaggcccggc agcccgttcc atcaagatcg atctgccccc gttcacgctg
      241 gtgggcgcca ctacccgtgc cggcctgctg acctcgccgt tgcgcgaccg tttcggtatc
      301 gtgcagcgcc tggaattcta ttcagtggaa gagttgtccc gcatcgtcag tcgttccgct
      361 gccattttgg gtgtggcgat ggacgcagag ggcgccactg aaatcgcccg tcgcagccgg
      421 ggcacaccgc gcatcgccaa tcgcctgctg cggcgtgtgc gggactatgc ggaagtgaag
      481 ggggatggcc gcgttaccat cgagactgcc ggcaaggccc tggatatgct caaggtggat
      541 tctcatggtt ttgataccat ggaccgtcgt ctgctgctga ccattatgga aaaatttgac
      601 ggcggccccg tgggtgtgga aagtctggcg gcggccatca gtgaggaagc cggtaccctg
      661 gaggacgtgg tggaaccgtt cctgatccag cagggtttca tgatgcgcac tccccggggc
      721 cgggttgtta cccgccaggc atggctgcac tttggcttga accctccggc ccgcacgaca
      781 acagcgacgc aggatctgtt taccaatgaa tgaattttcc atcagcctcc gggtctatct
      841 ggaagataca gacgccggtg gcattgttta ttacgtcaat tatctgaagt tcatggagcg
      901 cgcccggacc gagctgctgc gcagcctggg gtttgagttt gcggctctca gtgcagagca
      961 gttgcagttt gtggtgcaca gcgtcgatgc ccaataccgc aagccggcgc gcatggacga
     1021 cgaattgatc atcaccgcgg tggttgaaca gctggcccgt acctatatcg tcttccacca
     1081 gcaggtccgt cgtggcgacg agattctgtg tgaagcgcat atcaaggtgg cctgtgtcga
     1141 caccgcccag ttaaggccgg cagcgcttcc cgcgccggtc aaactccagt tttcccaggc
     1201 gcggctgccg cccgggccga attcgcatta accaaggaga caattgcacg tggaatccat
     1261 gtccatcctg agtctgatcg ccaacgccac catcccggtc aagattgtga tgcttatcct
     1321 gctgtcgctg tcggtggcat cctggtacat gatctggcag cgccagacag tcatgtcgaa
     1381 aacccagaag gcccagacca gcttcgagga acgcttctgg tccggcatcg atctcagccg
     1441 cctgttcgtg caggtcaaca ctgacccgaa tcctcactgt ggccaggaaa acatcttccg
     1501 cgccggcttc aaggaatttg cccgcctgcg caagaatctc aacagcgacc cggattccgt
     1561 catggaaggc accgagcgcg ccatgcgtgt ggcgctgctg cgggagcagg aaaaactgga
     1621 aacccatctg ccgttcctgg ccaccgttgg ttccaccagt ccgtacatcg gcctgtttgg
     1681 tactgtgtgg gggatcatgc attccttcct caatctggcc aaccagcagc aggccacact
     1741 ggctgccgtt gcgccgggta tcgccgaggc gttgatcgcc acggccattg gcctgtttgc
     1801 cgcgattcct gccgtggtgg gttacaaccg tttctccgcc gtggtggacc gcctgctgca
     1861 cagctatgaa accttctccg acgagttctc cagcatactg caccgcaaag cccattccgg
     1921 cgaagcggac gacaagtaag ccggcagcga gcagcaactg acatggcgca gaaaaaaacc
     1981 acagtccggc gcaagccgat gtccgaaatc aacgtcgtgc cctacatcga cgtcatgctg
     2041 gtattgctgg tggtcttcat gatcaccgcg cccatgatga cggcgggcgt gaaagtggat
     2101 ctgccggatg cggatgccaa acccatagag atgcccaaag acgaggagtt tgtcatcgtc
     2161 tcggtcaatc aggctggcca gtattatctg agcgttggcg gcgacaccaa ggaaccccgt
     2221 cccatggagg aagtggcgga catggtgggc aagatcaagc gcaacaagcc tgccaccccc
     2281 gttctgatgg aaggcgacca ccacgccgcc tatggcctgg tggtgcagat gatggtggcc
     2341 ctgcagaagg tcggcgtgaa tgacgtcggg cttgtcaccg aaagcccgca caaggagccg
     2401 taagtttggt ttcccaacgc cttgatctgg ccgccctgtc caagtcgctg ctggtgcatg
     2461 cgctggtcat cggcatgttc ttcgtcagct ggaaaagcac ggatgaagtg gtgacggcca
     2521 aggccatgcc catgcacatc acggccatgg tcatggaaaa accccagccg aagaccgcac
     2581 ccaaaccgca gcccaagccg gcagcgcaac ccaagcctcc agagcccaag ccggaaccga
     2641 aaaaaccgga gcccaagaag ccggaaccca aaaagcccga gccgaagaag cccgagatca
     2701 agaagcctga gccc
//
LOCUS       DASUEG010000407         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_623329, whole genome shotgun sequence.
ACCESSION   DASUEG010000407 DASUEG010000000
VERSION     DASUEG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623329"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1855
                     /locus_tag="VFX11_07540"
     CDS             251..1855
                     /locus_tag="VFX11_07540"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX4938511.1"
                     /translation="MTQEFDYLVLGGGSAGCTVAGRLSEDPATSVAVIEAGKGGDGWL
                     VNVPAAVVLMVPTKINSWALDTVPQPGLDGRTGYQPRGKALGGSSAINAMVYIRGHRW
                     DYDHWAALGNQGWSWDDVLPYFKKSEHNYTFTDELHGTDGPLPVSNLRTDNPVHQLYL
                     EAARQAGFPIVEDFNGVSQEGIGIYQVTQKDGERWSAARAYLLPHLATRSNLRVELQA
                     NIQRILFDGKRAAGVEFVQNGQTRVLRARKEVILSAGAFQSPQLLMLSGIGDGEELQR
                     LGIRTLHHLPGVGHNLQDHPDFVFGYKTKSLDLMGLSFPGAGRMLGEIQRYRKERRGM
                     ITSNFAEGGGFLKTRADLPAPDIQLHFVTALVEDHARKMNLGHGYSCHVCLLRPKSRG
                     RVGLLSADPNQPPLIDPNFLGEAEDVRDLVEGFKLTRKLMDAPALAALRTEDCFTANV
                     RTDGDIEQVLRQRVDTVYHPVGSCRMGVDEMAVVDPQLRVRGVEGLRVVDASVMPTIV
                     GGNTNAPTIMIGEKAADLIRAGARKG"
     gene            complement(2101..2847)
                     /locus_tag="VFX11_07545"
     CDS             complement(2101..2847)
                     /locus_tag="VFX11_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4938512.1"
                     /translation="MIRLKNPLSIRAAKRNVLSTTMSLASVWVVTVSALPADAAPVID
                     QSVIYPASGGISSYSIYETPTNQYVLAQTFTAGMSGQLTAVNLGLFDKAIENLTINIL
                     GTSGGLPNLTAPLMSGTLTPSGVDNDPFSLETVDFSSYLFDVVAGEMYAIMLSSTTPF
                     QQEYNWTRGSFDTFTGDDLSPADPYAGGVGYYSNNGGSTWNALTDFSQDFSFQTIVDP
                     NPVPEPGSIGFMLAGLAVYRFLSSRRRVKV"
     gene            complement(3135..3836)
                     /locus_tag="VFX11_07550"
     CDS             complement(3135..3836)
                     /locus_tag="VFX11_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX4938513.1"
                     /translation="MTHILHIDASARPGLAGADAHGSLSRKLTHRFVSRWLSRRPDNT
                     VTHRDIGAIPPSFINRDWIQAAFTPTDRQEPWMAQVLAESDRLIDELVAADVLVIGAP
                     LYNFGMPAALKAWVDLVVRMGRTVEYNPARPEDPFVPKLNDRPRHAVILSARGGAGFE
                     PGAALAHLNHLEPGLTTVLGFIGITNVHRIAVENQEAGGELLANSVKEAERRVDELVK
                     KLQMELLTHPEAEPV"
     gene            3969..4868
                     /locus_tag="VFX11_07555"
     CDS             3969..4868
                     /locus_tag="VFX11_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4938514.1"
                     /translation="MTVRWLPSLSALRAFEAAARHESAKQAAEELSVTATAISHQIRG
                     LEEALGVALFVRKPRQLELTPQGRELQQVLEMAFDSVGAVVERLKRMPARQAVTISTT
                     PAVAVRWLLPWVCMLRDSHPDIDLRFHASHEPVALDGVTADLAIRYGDGRWPGLIAEK
                     LFDNLFIPACSPHLNLHDVADLPKHPLIHFRPRGASSSPMDWAGWQKQARVPELDVSA
                     GLVFSDETHAISAAIGGQGVALMSRQLIEDELKEGRLMQPFGPDLPGKPFFLVYPENR
                     RNAPTILAIRDWVMNVPGGICRN"
     gene            5002..>5365
                     /locus_tag="VFX11_07560"
     CDS             5002..>5365
                     /locus_tag="VFX11_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938515.1"
                     /translation="MYKSALAALAFGLSSLAAGHAVADTTLLRCFYKTSADSTNTDFS
                     YEWGLKTSSSSLPVTLSGNWWKSAAMNDPANMFFTNTSQATLKSYCQNAFTKKGIARP
                     LMRMAAGSTSNSLNYTIWS"
BASE COUNT         1105 a         1515 c         1641 g         1104 t
ORIGIN      
        1 cccggctggg ctgagtccgt cgtgtcggat ggctagagtc gatcatttta cagccgcgac
       61 cgcccggttt ctgcagaatc accccggttc aaaagcggca aaaatgccac taccgcaggc
      121 ttgacagctg gcgcccttgc acagcgtccc ggattgaaac agcgggactt ttatggatca
      181 atgagctgcg gcccctgatg acagccaacg acaagaaaca acaacaagaa ctcacagcgg
      241 agaacacgga atgacgcagg aatttgatta tctggtactg ggtggcggtt cggcagggtg
      301 tacggtcgca gggcggctgt cggaagatcc ggccacttcg gtagcggtga tcgaggcggg
      361 caagggcggc gacggctggc tcgtgaacgt gcccgccgcc gtggtgctga tggtgcccac
      421 caagatcaac agctgggccc tggacaccgt gccccagcca ggtcttgacg ggcgcaccgg
      481 ttaccagccc cggggcaagg cgctgggcgg ctcatcggcc atcaacgcca tggtgtacat
      541 ccgtggccac cgctgggatt atgatcactg ggcggcgctg ggtaaccagg gctggtcctg
      601 ggacgacgtg ctgccctatt tcaagaagtc ggaacacaac tacaccttca cggacgaact
      661 gcacggtacc gacggccctc tgcccgtgag caatctgcgc actgacaacc cggtgcacca
      721 gctgtatctg gaagcggccc gtcaggccgg cttccccatc gtcgaggatt tcaacggtgt
      781 cagccaggaa ggcatcggca tctaccaggt cacccagaaa gacggcgagc gctggagtgc
      841 ggcgcgggct tatctgctgc cccacctggc cacgcgaagt aatttacggg tggaactgca
      901 ggccaacatc cagcgcattc tgttcgacgg caagcgcgca gcgggcgtgg aattcgtgca
      961 gaacggtcag acccgcgtgc tgcgggcgcg caaggaagtg atcctgagtg ccggtgcgtt
     1021 ccagtcgcca cagctgctga tgctgtccgg catcggtgac ggcgaggagc tgcagaggct
     1081 cggcatccgc accctgcatc atctgccggg cgtgggccat aacctgcagg accacccgga
     1141 tttcgtcttc ggctacaaaa ccaaaagtct ggacctgatg ggcctgtcat ttcccggcgc
     1201 tggtcgcatg ctgggcgaga tccagcgcta ccgcaaggaa cggcgcggca tgatcacctc
     1261 gaactttgcc gagggcggcg gcttcctgaa aacccgtgcc gacctgccgg cgcccgacat
     1321 ccagctgcat ttcgtcaccg cgctggtgga ggatcacgcc cgcaagatga acctgggcca
     1381 cggctattcc tgccacgtct gtctgctgcg gccgaaaagc cggggacggg tggggcttct
     1441 cagtgccgat cccaaccagc cgccactgat cgatcccaat ttcctgggcg aggcggagga
     1501 tgtgcgcgat ctggtggaag gcttcaagct gacccgcaaa ctgatggacg cgccggcact
     1561 ggcggcattg cgcaccgagg attgcttcac cgccaatgtg cgcaccgacg gcgatatcga
     1621 gcaggtgttg cggcagcgcg tggatacggt ttaccacccg gtgggttcat gccgcatggg
     1681 tgtggacgag atggcagtgg tggacccgca actgcgggtg cggggtgtgg aggggctgcg
     1741 agtggtggat gcgtcggtca tgcccaccat tgtgggcggt aataccaatg cgccgaccat
     1801 catgatcggg gagaaggcgg cggatttgat tcgcgcgggt gcgaggaagg gatgactggt
     1861 gcatgggaag gcctctgagc ccatgggaat gggtgcgcat caccggaggc atttcaaaca
     1921 gccgcacctg atcatgcctg gcgtgatgag ctgaaaatga ttggccatct ccgtaacaat
     1981 ggggcgctga agaaccctgc ggccagcccc gcaatggcgt tggacatcat gaatcgatgc
     2041 cacgcgacca tgatccctga agaagcagcc ggaaaacgac tgcttcttca tttattttct
     2101 tcagaccttt acacgccgac gtgacgacaa aaaccggtag acggccagac ctgcaagcat
     2161 gaatccgatg gatcctggtt ccggtaccgg gttcggatca acgatggttt gaaagctgaa
     2221 atcctggctg aagtccgtca gcgcattcca ggttgaacca ccattattac tgtaatatcc
     2281 cacgccgcct gcataggggt cggccggcga aaggtcatcg ccggtaaatg tgtcgaatga
     2341 gccgcgggtc cagttatatt cttgctggaa tggcgtcgtg ctggagagca tgatggcgta
     2401 catctcgccg gcaacgacat caaacaggta cgaggaaaaa tccactgtct caagggaaaa
     2461 gggatcattg tcaacgccgg atggcgtcag ggttcccgac atcagggggg cggtcagatt
     2521 gggcaggccc ccgctggtgc ccagtatgtt gatggtgaga ttttcaattg ccttgtcaaa
     2581 gagacccaaa ttgacggcag tcagttggcc cgacattcct gcggtgaacg tttgcgcaag
     2641 gacatactgg ttcgttggcg tttcataaat gctgtaggac gatatgccac cacttgctgg
     2701 atagataacg gattggtcaa taaccggtgc cgcgtctgcc ggcagcgcag agaccgttac
     2761 cacccacacc gatgccagcg acatcgtcgt ggacaggaca tttcgtttcg cagcgcggat
     2821 tgatagagga tttttcaacc tgatcatgtc gacctcctta ttttcaatag tttgctttct
     2881 tttgaccttc ctacttcaaa aaaatgtagc tggattcagt tcttaacagc tgtatcaaag
     2941 actgaatgag cgagcatttt caaactgccg accagcattt caatgcagtg ccagggttgg
     3001 agaatgttga tttcccgatt gagactttcc aagaatcaaa cgcaaaataa cagtaactag
     3061 ttcggcggtg gaataggaaa tataacccca cgaatggcat gcgcttcttg ctctcaatca
     3121 aagagtgaaa tttcttaaac cggttctgcc tcgggatgtg tgaggagttc catctgcagt
     3181 tttttcacta actcgtccac ccgtcgttcc gcttctttaa ccgagttcgc cagcagctcg
     3241 ccaccggctt cctggttttc caccgcgatc cggtggacgt tggtgatgcc gatgaacccg
     3301 agcacggtcg tcaggcccgg ttcgaggtga ttcaggtgag ccagggctgc gcccggctcg
     3361 aaaccggcac cgccccgggc agaaagaatg accgcatggc gtggccggtc gttcagcttg
     3421 gggacgaacg ggtcttcggg cctggccggg ttgtactcca ccgtccggcc catgcgtacc
     3481 acgaggtcaa cccaggcttt gagcgcggcg ggcataccga agttgtagag tggcgcgcca
     3541 atcaccagga catccgcggc aacgagttca tcgattaacc ggtcgctttc cgccagtacc
     3601 tgcgccatcc agggctcctg acggtccgtc ggtgtgaagg cggcctggat ccagtcccgg
     3661 ttgatgaagg agggggggat ggcgccgatg tccctgtggg tgacggtatt gtcggggcgg
     3721 cgggataacc agcggctgac gaaccggtga gtgagttttc gactgaggga accgtgtgca
     3781 tccgcgccgg caaggccggg tcgggcgctg gcatcgatgt gcaggatatg ggtcatggcg
     3841 aggctcctct tgcagggatt gatatgagtt gagttcatct gtgtttggca gcaaactcag
     3901 gttatcgccg cccatatcaa ggcgacaaac gatcatttct tccacctaca gatgagccag
     3961 attcaatcat gactgtgcgt tggttaccct ccctttccgc cctccgggct ttcgaagcgg
     4021 cggcgcgtca tgagagcgcc aagcaggctg ccgaggagct ttccgtaacg gccaccgcca
     4081 tcagccacca gattcgcggt ctggaagagg cgctgggggt tgccctgttc gtgcgtaagc
     4141 cccggcagct tgaactgacg ccccagggcc gggagctgca gcaagtgctg gaaatggcat
     4201 tcgatagcgt cggcgctgtt gtggagcgtc tgaaaaggat gcccgcgcgt caggcagtca
     4261 cgatctcgac gacgccggcg gttgcggtgc gctggttgct gccctgggtg tgcatgctgc
     4321 gggattcgca tccggatatt gacctgcgct tccacgcgtc ccacgagcct gttgcgctgg
     4381 atggggtgac agcggatctg gccattcgtt acggcgatgg tcgctggcct ggtctgatcg
     4441 cggagaaact gttcgacaac ctcttcatac ccgcctgcag tcctcatctc aatctgcacg
     4501 atgtggcgga cctgccaaaa catccgctga ttcacttccg gccgcggggc gcatcgtcgt
     4561 cgccgatgga ttgggccggc tggcagaaac aggcgcgggt gccggaactg gatgtcagcg
     4621 ccggattggt attctcggat gagacccacg ctatctctgc tgccatcggt ggtcagggcg
     4681 tcgcattgat gagccgccag ctgatagagg acgagctgaa ggaaggccgg ctgatgcaac
     4741 ctttcggccc ggatttgccg ggcaaaccct tcttcctggt ctatccggaa aaccgacgca
     4801 atgccccgac cattctggcg atccgcgact gggtgatgaa cgtgccggga gggatttgca
     4861 ggaattgaaa atacaccaaa gcaaaagcgc gactgcgtca atccctcttt cgtcttaccg
     4921 atcctgttgg cgcgaatgtt agcgtgacac tgagccttcc ggcccgtcac ttttcgcatt
     4981 ttcggataca ggagttccac catgtacaag tctgcgcttg ccgctcttgc ctttggtctg
     5041 tcatcactgg cagcaggcca tgcggttgcc gataccacct tgttacgctg tttttacaaa
     5101 accagcgcgg attccacgaa cacggatttc agttatgagt gggggctgaa aaccagctcc
     5161 agctcgctgc ccgtcacgtt gtcaggcaac tggtggaaga gcgcggcgat gaacgatccc
     5221 gccaatatgt ttttcaccaa tacctcgcag gccacgctga aatcctattg ccagaatgca
     5281 ttcaccaaaa aagggattgc ccggccgctg atgcgcatgg cagcgggttc cacctcgaac
     5341 agtctcaatt acacaatctg gagta
//
LOCUS       DASUEG010000408         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_405811, whole genome shotgun sequence.
ACCESSION   DASUEG010000408 DASUEG010000000
VERSION     DASUEG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405811"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFX11_07565"
     CDS             complement(<1..1063)
                     /locus_tag="VFX11_07565"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4938516.1"
                     /translation="MDRILVDFIKALRGTGVRTSTAETLDAVAAVKIVGYSNRDYLKS
                     ALATTLAKTEDDHQKFNVCFDQFFSFNALDAFAEKQPAQKPPESTAARLLQSLRPQRS
                     APAQPQSPLGQMLMQGNPQQVAVALAQAGAAANVNQIRVLTQKGLYGRRMMMQMGLAE
                     MEQEMWSAEQSTDPQQQQLAQNLREAREQLREEVRQYVERQLLLQAAGESRALRESIM
                     LETRLDNLREFKDVQILVRKLAKRLVATHSRRRKIFQRGRLDVRTTIRRNIPYSGVLL
                     ETHWKTKKVDRPKVMAICDVSGSVSQYAKFLLMFLYSLTDVLKNVRAFAFSSDLVEVT
                     QLFDQFDLNDAVDATLKRVG"
     gene            complement(1223..2152)
                     /locus_tag="VFX11_07570"
     CDS             complement(1223..2152)
                     /locus_tag="VFX11_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4938517.1"
                     /translation="MTHDLSVEKIRQGFAHAGYICNPAIAMTMLLAYKLEKPVLVEGP
                     PGVGKTELAKATATLLNYDLIRLQCYEGLDESKALYEWKYGKQLLYTQLLKEKLGDLL
                     KGAHDLPSSIARLHEFGDIFYSEDFLEPRPLLQALRAEKGTVLLIDEIDKADHEFESF
                     LLEMLSDYQISIPEIGTIKARHHPIVFLTSNNTRELSDALKRRCLHLYIPFPDAALER
                     TIIQTRVPGVNEALREQLVGFVQQLRQLDLKKQPAISETIDWARTLLLLHTEALSAET
                     VESTLNVLLKHEEDITSVKDDLRGLLAKLVQRK"
BASE COUNT          388 a          651 c          718 g          522 t
ORIGIN      
        1 cgcccacccg tttcagggtg gcatcgacgg cgtcgttcag atcaaactgg tcgaacagct
       61 gggtgacttc caccagatcg gacgagaaag caaaggcgcg cacgtttttc agcacgtcgg
      121 tgaggctgta taaaaacatc agcaggaatt tggcgtactg gctgaccgag ccgctgacat
      181 cgcagatcgc catgactttc gggcggtcga cctttttggt tttccagtgg gtttccagca
      241 gcacgccgct gtagggaatg ttgcggcgga tggtggtgcg cacgtcgagc ctgccacgct
      301 ggaaaatctt gcgccggcgc gaatgggtgg ccaccaggcg tttggccagt ttgcgcacca
      361 gaatttgcac gtccttgaat tcgcgcaggt tgtcgaggcg ggtttccagc atgatggatt
      421 cgcggagcgc gcggctttcg cctgcggcct gcagcagcaa ttgccgctcg acatactggc
      481 gcacttcttc acgcagctgt tcgcgagctt cccgcagatt ttgcgccagc tgttgctgct
      541 gcgggtcggt ggactgttcg gcgctccaca tttcctgttc catttccgcc agccccatct
      601 gcatcatcat gcggcggccg tacaggcctt tctgcgtgag cacgcggatc tggttgacgt
      661 tggcggcggc gcccgcctgg gccagtgcca cggcgacctg ctgcggattg ccctgcatca
      721 gcatctgacc cagcggtgat tgcggttggg ctggagcgga gcgctgtggt cgcaggcttt
      781 gcagcaggcg cgcggcagtg gattccggtg gcttttgcgc gggctgtttt tcagcgaacg
      841 catccagggc attgaaggaa aagaattggt cgaagcagac gttgaatttc tggtggtcgt
      901 cttcagtttt ggccagggtg gtggcgagtg cggatttcag gtaatcgcgg ttgctgtagc
      961 cgacgatctt gacggcggcc acggcgtcga gtgtttccgc cgtggaggtg cggacgccgg
     1021 tgccgcgcag ggccttgatg aaatcaacga ggatgcggtc catttcaata atgtgccttc
     1081 tacagtcgcc acattgaatc agcggcaagt gcgctcgata ccggacacgc cgtaaaccca
     1141 tccctggggg ctgctccgcg gcatccctgc cgcagagcgt ccggaatcga gcgcacttgc
     1201 cactgcttca atgtcccggt tgtcatttcc gctgcaccag cttcgccagc aagccgcgca
     1261 gatcgtcctt cacgctggtg atgtcctcct cgtgcttgag cagcacgttc agcgtggact
     1321 ccaccgtctc tgccgacagc gcctccgtgt gcagcaacag cagcgtgcgc gcccagtcga
     1381 tggtttcgct gatggcgggc tgttttttca gatccagttg ccgcaattgc tgcacgaagc
     1441 ccaccagctg ttcccgcagt gcttcgttta cgccgggcac gcgggtctgg atgatggtgc
     1501 gttccagcgc ggcgtcgggg aacggaatat aaagatgcag gcaacgccgc ttcagcgcgt
     1561 cgctcagttc gcgcgtgttg ttgctggtca gaaaaacgat gggatgatgg cgggccttga
     1621 tggtgccgat ctccggaatc gagatctggt agtccgacag catctccagc aggaaggatt
     1681 cgaattcgtg atcggccttg tcgatttcgt cgatcagcag cacggtgcct ttttccgcgc
     1741 gcagtgcctg cagcagcggg cgcggttcca ggaaatcctc ggaatagaaa atgtcgccga
     1801 attcatgcag gcgggcgatg gacgacggca ggtcgtgggc acctttcagc agatccccca
     1861 gtttttcctt cagcagctgg gtgtacagca gctgtttgcc atatttccat tcatacagtg
     1921 ccttggattc atccagccct tcgtaacatt gcaggcggat caggtcgtaa ttgagcaggg
     1981 tggcggtggc cttggccagt tcggtcttgc ctacgcccgg cgggccttcc accagtacgg
     2041 gtttttccag cttgtacgcc agcagcatgg tcatggcgat ggcggggttg cagatgtaac
     2101 cggcatgcgc gaagccctgg cggatttttt ctacactgag atcgtgggtc atgtcgtggt
     2161 ttgcgtgtgc cgggaacggc cttaaaccat aacacaaaac cgccgcgtcg ccttgttccc
     2221 ctgcgggcct tgggacacaa tggtgcgctg ttttcgcccg gagatgcctg ccttgcccc
//
LOCUS       DASUEG010000409         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1526114, whole genome shotgun sequence.
ACCESSION   DASUEG010000409 DASUEG010000000
VERSION     DASUEG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526114"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VFX11_07575"
     CDS             complement(<1..93)
                     /locus_tag="VFX11_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX4938518.1"
                     /translation="MPELPHVSGAEVVRALERLGFIAVRQSGSHV"
     gene            complement(86..298)
                     /locus_tag="VFX11_07580"
     CDS             complement(86..298)
                     /locus_tag="VFX11_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX4938519.1"
                     /translation="MELTAVLTPAEEGGFVALNPETGTTTQGETVEEAVANLREATAL
                     YLSEFPLPTTGHPVVTVFTIPEPAGA"
     gene            complement(352..600)
                     /locus_tag="VFX11_07585"
     CDS             complement(352..600)
                     /locus_tag="VFX11_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX4938520.1"
                     /translation="MARLPRYVIPGQPQHIIQRGNNRQVIFAAEADFQFFRDVLVEAA
                     SKHDLAIHAYVWMTTHVHLLANPGNGDTISEMFDDSRV"
     gene            complement(708..1568)
                     /locus_tag="VFX11_07590"
     CDS             complement(708..1568)
                     /locus_tag="VFX11_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938521.1"
                     /translation="MSGYNINLCDYAVAFVDLLGQKADMPGRHLPNNKQEAIALVKCS
                     VGKIVGMQRLFESFYEAHSSGTTLYSQLPKHIQDQALDMAPGELKRQMFSDGMVVYIP
                     LGRGIIKSPINSIFVLLIASGLLCATGLAAEGPVRIGIDVAWAVEYKPGELYGSALAH
                     AYNLESEVAKWPRVVVGEGLLGYLQHYAESPGSDISSQFRREMAALCRGLISQDIDGQ
                     YILHYLGSGFAKAVQSGIDGDIVHRAREFIDFQLAKWEKEGNSVLARRYEIVKQYYEQ
                     HCPGANGYGA"
     gene            1697..1928
                     /locus_tag="VFX11_07595"
                     /pseudo
     CDS             1697..1928
                     /locus_tag="VFX11_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818233.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          457 a          588 c          556 g          448 t
ORIGIN      
        1 gacgtggctg ccactctggc gaaccgcgat gaagcccagg cgctcgaggg cgcgcacaac
       61 ctcagccccc gacacatgag gcagctcagg cacccgccgg ctcaggaatg gtgaagaccg
      121 ttacgacggg atgcccggta gtggggagcg ggaactcaga caggtacagg gctgtggcct
      181 cgcgcaggtt ggccaccgcc tcctcgacgg tctcgccctg tgtggtggtt ccggtctcgg
      241 gattgagcgc aacaaagccg ccttcttcgg cgggcgtaag gactgctgtg agttccatga
      301 ggtgccctcg ctggccaagt gcagtgattg tcgcagaaca gaacaacggg gtcagacacg
      361 actatcatca aacatctcgc tgatggtgtc gccattgccg ggattggcca gcaggtgaac
      421 gtgggtggtc atccagacat aggcatggat cgccagatcg tgcttgctgg cggcctcaac
      481 cagcacatca cgaaagaact gaaaatcggc ctcggcggcg aagatgacct ggcgattgtt
      541 gccgcgctga atgatgtgct gtggctggcc tgggatgacg tagcggggaa ggcgtgccat
      601 gaggctttcc cttcaacggt aaatccggtg ctcagcctgg catgaattaa ttgtgcctga
      661 ccccattttc cttgtttccg tgcgttctgt gatggctaac gtcccagtta ggcaccgtac
      721 ccattcgcgc ccgggcaatg ctgctcatag tattgcttga caatctcgta gcgcctagct
      781 agcacactat ttccttcctt ctcccacttc gccaactgga aatcgatgaa ttctcttgcg
      841 cgatggacga tgtcgccatc gatgccggat tgcacggcct tggcaaaccc cgaacccaaa
      901 tagtgaagaa tgtattgtcc atcgatgtct tgagaaatca gtccgcggca caacgcagcc
      961 atttctcttc ggaattgact cgaaatatcc gagccgggtg attctgcgta gtgctgcaag
     1021 taaccaagca atccttcccc aactaccact ctcggccact tcgcaacttc gctctccaaa
     1081 ttgtatgcat gagcgagtgc ggaaccgtaa agttctccag gtttgtactc gactgcccac
     1141 gccacatcaa tgccgatacg aacaggcccc tctgcagcca acccggtggc acaaagcaac
     1201 cctgatgcga tgagcaacac gaatatgctg ttgatcggag atttgataat tccccggcca
     1261 agcggaatat agaccaccat gccatcggag aacatttggc gcttcaactc ccctggagcc
     1321 atgtctagcg cttgatcctg aatgtgcttc ggcagctggg aatatagggt agttccggat
     1381 gagtgcgcct cgtaaaacga ctcgaagagc ctctgcatac cgacaatctt tccgaccgag
     1441 cactttacca gcgcgattgc ctcctgcttg ttattcggta aatggcgccc cggcatatct
     1501 gctttctggc cgagtaaatc gacgaacgcg acggcgtagt cgcacaggtt aatgttatat
     1561 ccgctcatgg taaccaacgc aaaatataca gcttgacaga tgtatcttca gcttcgacgc
     1621 agatgtatat cacccggaaa tctttcacaa acttatgatc tcggatattt gctttaagtt
     1681 ataaagtgga tacaggctgc catcaggtac cctcatacag catggtccca agctgctcga
     1741 tgaggtgcgt gaccaaattc gcgtcaagca ttacagcatc cgcacggaga cgcaatacgt
     1801 gcagcgggag ccccagcatt ctgtttcacg acaagcggca tccgagcgag gtgggggctg
     1861 gggagatcga ggcgtttctg tcacctcttg cggtcgatgg gaaggtcgcc gcggcgaccc
     1921 agaaccagcg cgctgtccgc gttgctgtta cgccatatca accggccgcc tgcgcgcacg
     1981 cggcggcgca gtgtcacctg ttgtctctca ccccgaatca ggacgtttga gtatcaataa
     2041 cccggacac
//
LOCUS       DASUEG010000410         6788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_311962, whole genome shotgun sequence.
ACCESSION   DASUEG010000410 DASUEG010000000
VERSION     DASUEG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311962"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1519
                     /locus_tag="VFX11_07600"
     CDS             29..1519
                     /locus_tag="VFX11_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX4938522.1"
                     /translation="MSSKQILIGKGDIPCFLEGRYANRHGLIAGATGTGKTITLQILA
                     EGFSDLGVPVFLADIKGDVAGISQAGAENPKLVERAAKVGLEDYRMQAYPTVFWDVFG
                     EQGHPVRATISDMGPLLLSRLMDLNEVQEGVLTLLFKYADDNGLLLLDLKDLRSLVSY
                     AADNTNELGKDYGNVTKQTLGILQRQLLALETQGADKFFGEPALKLEDFMQTTLEGRG
                     HINILAADKLYQQPRLYATFLLWLLSELFENLPERGDAELPRLVFFFDEAHLLFNDAP
                     KGLLEKVEQVVRLIRSKGVGIYFVTQNPLDLPDTVLGHLGNRVQHALRAFTPRDQKAV
                     KAAADTFRAKPGLDVATTITEMGVGEALVSTLIEGGIPSPVERAKIRPPRSRMGAITP
                     EERATIMQRSPFRSAYEQTLDRESAHEMLTRRAAQDAADTQAKAETVAAEKAAKTAAR
                     TAAKEPDTIWETAAKSAVRAASSELGRSVGRKLLRGLLGSLSKSIR"
     gene            complement(1547..2002)
                     /locus_tag="VFX11_07605"
     CDS             complement(1547..2002)
                     /locus_tag="VFX11_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX4938523.1"
                     /translation="MNSSASVWRPVLEPRPSRIQRIILILFYVLALVAVLQAVMPFEW
                     QVAAVSAWFGLLLYELTSWRHPLSVVLLHERIGDWWLADAEGNASRVSLHRSMVWRYL
                     VVLDFRILEGAGPRRQRVVLFPDSVTADEFRRLRVRLLQGPPPGKTLRE"
     gene            complement(1965..2228)
                     /locus_tag="VFX11_07610"
     CDS             complement(1965..2228)
                     /locus_tag="VFX11_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011864849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX4938524.1"
                     /translation="MTHELTDHERRIAWHCRRGLKELDVFLNPFMEGHYRGLSDHDKA
                     VFERLLACEDIDLFHWFMGDEPVPDPELERMIELIRVRVAAGT"
     gene            2227..3318
                     /locus_tag="VFX11_07615"
     CDS             2227..3318
                     /locus_tag="VFX11_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX4938525.1"
                     /translation="MTSSSRPGLVPGNRELYACFSGKSPYNVAMTSPGIPLMPFDWSA
                     LPASPSTDIAPANAQLLITPLPGLASLQVSGPDSYKFLQGQCTTDFREVEQGKVLPGA
                     ICSLKGRALFTFLAIPSGETVYLLLPQDQLADAHAHLKKYSIFSKVTLTADSTTPLLG
                     IQGPVSTAALSNLLQTEPVAGQSASNDGIVAAALAKDRYLLILPAEQANAVASKLLSM
                     HALLTTENAWHLSDIRAGLANVQATTRDQFQPQELNYPALSGVSYNKGCYTGQEVVAR
                     LYFRGKLKQRLYRLSGEGQPSEETSIHAGEQHVGDVVNIARSADNGIELLAVVKNAAV
                     NNGTLTLGRHGPALHVQDLPYALDADKEE"
     gene            complement(3324..4436)
                     /locus_tag="VFX11_07620"
     CDS             complement(3324..4436)
                     /locus_tag="VFX11_07620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4938526.1"
                     /translation="MSDSPVVLLEERALKNGRVLAVATLNSERSLNSLSLDMISLLLP
                     AMRRWAADERVVAVFLQGAGEKAFCAGGDVRRICVGVRENGIDDPYAQQFFAEEYKLD
                     YLIHTFPKPVIVWGQGIVMGGGIGLMCGASHRVVTETSRLAMPEITIGLYPDVGGSYF
                     LSRLPGRLGLFLGMTGVNLNAGDALFAGFADHAVASDRRQAVLESLAELDWVDDMAGR
                     KAQVSAVLEAEECPGLPASEVQSRLALINERAAADDLPTLFAALTVPAPDDAWLDRAA
                     RTLAKGSPTSAALILRQWQRGRDLSLADVFREELTLSTQCARHPDFIEGVRALLVDKD
                     NSPKWQPATVAGVTEDWIAGHYVEPWQGEHPLAGLV"
     gene            complement(4444..5139)
                     /gene="ung"
                     /locus_tag="VFX11_07625"
     CDS             complement(4444..5139)
                     /gene="ung"
                     /locus_tag="VFX11_07625"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4938527.1"
                     /translation="MSDTLQHVRMNPEWRAALEPEFLSSYMHELRRFLSAEKQAGKEI
                     YPPGSEMFNAFNTTPLSKVKVVILGQDPYHGPRQAHGLCFSVRRGIQPPPSLVNIYKE
                     IQSDLGIAPPRHGELTHWAAQGVLLLNSVLSVQAGLAASHQGRGWETFTDAVIRVLNE
                     ERDHLVFLLWGNYAQRKGRFIDARRHLVLKAAHPSPLSANRGGWFGCRHFSQANRYLE
                     AHGVAPIDWSLPD"
     gene            complement(5136..5648)
                     /locus_tag="VFX11_07630"
     CDS             complement(5136..5648)
                     /locus_tag="VFX11_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938528.1"
                     /translation="MSATTYYFKDIHDLLADAFTLYAKESMESYIDPFWSRANLWLSA
                     YPEGVREHPEQLAEVVAHLSGMGAEYVLLRLSSLRDNIIIDNAFRYAALTDARMHSLA
                     IQHDQRLMANFTRLLAYLGAHEVKDAARSLMCLIRRLEFEGMLSSHESFCPDVARQAI
                     SHQLRAMLSV"
     gene            6050..>6788
                     /locus_tag="VFX11_07635"
     CDS             6050..>6788
                     /locus_tag="VFX11_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX4938529.1"
                     /translation="MDRQERLTYIIRLLQDMSLNSMEIKARFPGVSPRTIERDLEQLA
                     QEGRIHQVRRDRSRNPTWETGERAPTIEVRWMNGRTAAAIKVMESCLATILPNTLLQD
                     LRPLFSRADIALKQAHNREYANWLQKIRIRPSLKEDENADVTSRHLGIIQEALLKSQA
                     IHVTERPDSMTAISRTIHPQGLLFSRSGAHLIATDDAAHDIPLQICIQDISEVELLDQ
                     PSNTPAGFNLDHWLDSRKKEEATELTAS"
BASE COUNT         1581 a         2228 c         1793 g         1186 t
ORIGIN      
        1 tctttgattc atgccccgag gattgaggat gagcagcaag cagattctga tcggcaaggg
       61 tgacataccc tgctttctgg aaggccgata cgccaaccgc cacggcctga tcgctggcgc
      121 caccggcacg ggcaaaacca tcacgctgca gatccttgcc gaaggtttct ccgatctcgg
      181 cgtgcccgtc tttctggccg acatcaaagg cgatgtggcc ggcatctcac aggccggtgc
      241 ggaaaacccc aagctggtcg aacgtgccgc caaggtaggc cttgaagact accgcatgca
      301 ggcctacccc accgtgttct gggacgtctt cggtgagcag ggccatccag tccgtgccac
      361 catttccgac atgggcccgc tgctgctgtc ccggctgatg gacctcaacg aggtgcagga
      421 aggcgtcctc accctgctgt tcaaatatgc cgatgacaac ggactgctgc tgcttgacct
      481 gaaggacctg cgctcactgg tctcgtacgc cgccgacaac acaaatgagc tcggcaagga
      541 ctacggcaac gtcaccaaac aaacgctggg catcctgcag cgccagttgc tggcactgga
      601 aacccagggc gcggacaaat tcttcggcga gccggcgctt aagctggaag acttcatgca
      661 gacgacgctg gaagggcgcg gtcacatcaa catcctcgcc gccgacaagc tgtaccagca
      721 gccacgtctc tacgcgacct tcctgctctg gctactctcc gaactgttcg agaacctgcc
      781 cgaacgtggt gacgcagaat tgccgcgact cgtgttcttc ttcgacgaag cccacctgct
      841 gttcaatgac gcccccaagg gcctgctgga aaaagtggaa caggtcgtgc gcctgattcg
      901 ctccaaaggc gtgggcattt atttcgtcac gcaaaacccg ctggatctgc ccgataccgt
      961 actgggccac ttgggcaatc gcgtgcaaca cgccttgcgt gcgtttaccc cgcgcgatca
     1021 gaaggcagta aaggccgcgg ccgacacctt ccgtgccaag ccagggctgg atgtagcgac
     1081 caccatcacg gaaatgggcg tcggggaagc gctggtgtcc acgctcatcg aaggtggcat
     1141 cccctcgcct gtggagcgcg ccaagatccg tccgccgcgc tcacgcatgg gcgccatcac
     1201 gccggaagaa cgcgccacca tcatgcaacg ctcgcccttc cgcagcgcct acgagcaaac
     1261 actggatcgc gaatctgccc acgaaatgct gacgcgtcgt gccgcacagg atgccgcaga
     1321 cacacaggca aaagccgaga cggtcgccgc cgagaaagcc gcgaaaacag ccgccagaac
     1381 tgctgccaaa gagccggaca ccatctggga gaccgctgcc aagagtgccg tgcgagccgc
     1441 cagctccgag cttggccgca gtgtcggacg caagctgctg cgcggactgc tgggctccct
     1501 gagcaaatcc atccgctaag ccatccgtgc cccttacagg tcaccgctac tcgcgcaagg
     1561 tcttgccggg tggcggccct tgcaacaacc gcacacgcaa acgcctgaac tcatccgccg
     1621 tcacgctgtc gggaaacaat acaacgcgct gacgacgagg ccccgctcct tccagaatac
     1681 ggaagtccag aacgaccaga taacgccata ccatcgaacg atgcaggctg acgcgcgacg
     1741 cattgccctc ggcatctgcc agccaccagt cgccgatgcg ctcgtgcagc aacaccacag
     1801 acaaggggtg acgccagctc gtcagttcgt acagtaaaag cccgaaccac gccgacacgg
     1861 cagccacctg ccactcgaac ggcatgacgg cctgcaacac cgccaccagc gccaggacat
     1921 agaaaagaat caggatgatg cgttggatgc gcgaggggcg aggctcaagt accggccgcc
     1981 acacggacgc ggatgagttc aatcatgcgc tccagctcgg gatccggcac cggctcgtca
     2041 cccatgaacc agtggaacag gtcgatgtcc tcacaggcca gcaagcgctc gaaaactgcc
     2101 ttgtcgtggt cactcagacc ccggtaatgt ccttccatga acggattcag gaacacatcc
     2161 agctctttga gaccacgacg gcagtgccag gcgatccggc gttcatggtc agtgagctcg
     2221 tgtgtcatga catcctcctc cagacccggc ctcgtgccgg gcaatcgcga attatacgct
     2281 tgtttttccg gcaaaagccc ctataacgtc gccatgacat ctcccggaat accgctcatg
     2341 cccttcgact ggtctgccct gcccgcctct ccgtccaccg acatcgcacc tgccaatgca
     2401 cagctgctca ttacgcccct accgggcctg gccagcctgc aagtcagtgg tccggacagc
     2461 tacaagttcc tgcaggggca atgcaccacg gacttccgcg aagtggagca aggcaaggtg
     2521 ctgccggggg ccatatgctc gctgaaaggg cgcgccctgt ttacctttct ggcaatcccg
     2581 tcaggggaaa ccgtttacct gctgctccca caagaccagt tggcggatgc ccacgcccac
     2641 ctgaaaaagt acagcatctt ctccaaagtc accctgacag ctgacagcac aacaccgttg
     2701 ctgggcatac aaggccccgt ctcgactgct gccttgagta accttctgca aaccgagccc
     2761 gtagcaggcc agtccgcctc caacgacggt atcgtggccg ccgccctggc aaaagaccgc
     2821 tacctgctga tccttcccgc ggagcaggct aacgctgttg catcgaaact gctgtcaatg
     2881 cacgccctgc tgaccaccga aaacgcttgg catctttctg acattcgtgc cggcctcgcc
     2941 aatgtgcagg caaccactcg cgaccagttc cagccacagg agctcaatta cccggcattg
     3001 tcaggcgtga gctacaacaa gggctgctat acaggacagg aagtggtggc gcgcctgtac
     3061 ttccgcggca agctgaaaca gcgcctctac cggctgtctg gcgaaggaca gccatcagaa
     3121 gagactagca tccatgcggg cgagcagcat gtcggggatg tcgtgaacat cgcacgcagt
     3181 gcagacaacg gcatcgagct gttggccgtc gtgaaaaatg cggccgttaa caacggcaca
     3241 ctcacactgg gacggcatgg tcctgcgctg catgtgcaag acctgcctta cgcgctggat
     3301 gccgacaaag aagagtaagc gcgttagacc agaccagcga gcggatgttc gccctgccag
     3361 ggctcgacat aatgaccggc aatccagtcc tccgtgaccc cagccacggt cgcaggctgc
     3421 cacttcggtg agttgtcctt gtcgaccagc agcgcccgca cgccttcgat aaagtccgga
     3481 tgacgcgcac actgtgtcga cagcgtcagc tcctcacgaa agacatcggc cagtgacagg
     3541 tcacgtcccc gctgccactg tctaagaatc agtgcagcag aggttggcga gcctttggcc
     3601 agcgtgcgcg ctgcacggtc cagccaggca tcatccggtg ccggtactgt cagcgccgca
     3661 aagagtgttg gcaagtcgtc tgccgcggcc cgctcattga tcagtgccaa ccgggactga
     3721 acctcggatg ccggcagccc cggacactcc tcagcctcca gcacggcgga cacctgagcc
     3781 ttgcggcccg ccatatcgtc tacccaatcc agctcagcca acgactccag caccgcctga
     3841 cgccggtcag atgccaccgc atgatccgca aaccctgcaa acaaggcatc ccccgcattc
     3901 aggtttactc ccgtcatgcc gaggaaaagg cccaaccgcc ccggcaggcg tgacaggaaa
     3961 tacgaaccgc ccacatctgg atacaagcca atcgtgatct ccggcatggc caaacgagaa
     4021 gtctccgtca ccacgcgatg ggaggcccca cacatcaaac cgatgccccc gcccatgacg
     4081 atgccctgcc cccatacgat caccggcttc gggaacgtgt ggatcagata gtccagcttg
     4141 tattcctccg cgaagaattg ctgcgcgtag ggatcatcaa tgccgttctc gcgcacaccg
     4201 acacaaatcc gacgcacatc ccctccggca caaaaggcct tttcgcccgc cccctgcagg
     4261 aaaaccgcca ccacgcgctc atctgctgcc caacgcctca tggccggcaa cagcaggctg
     4321 atcatgtcca ggctgagtga attcagggag cgctcgctat tcaaggtggc aaccgcaagc
     4381 acccgcccgt tcttcagagc ccgctcctcc agcaacacga caggactatc agacatcact
     4441 acctcaatcc ggtaacgacc agtcgatggg cgctacacca tgcgcctcca gatatcgatt
     4501 ggcctgtgaa aaatggcggc agccaaacca gccaccccga ttggctgaca gcggcgacgg
     4561 atgcgccgcc ttcagcacca gatgcctgcg ggcatcaatg aaacgacctt tgcgctgggc
     4621 ataatttccc cagagcagaa acaccagatg gtcgcgctct tcgttcagca cacgaatcac
     4681 ggcatcggta aacgtttccc agccacgccc ctgatgtgaa gccgccagcc cagcctgcac
     4741 ggacaacaca ctattgagca gcaatacccc ttgtgcagcc caatgcgtca attcgccatg
     4801 gcgaggagga gcaatcccca ggtcagactg gatctccttg tagatattga ccagagaagg
     4861 cggaggctga atgcctcgcc ggacggaaaa acacagaccg tgagcctgac gcggcccgtg
     4921 atagggatcc tgccccagaa tcacgacctt cacttttgac aaaggcgtgg tattgaaggc
     4981 attgaacatc tcactcccag gcggatagat ctcctttccc gcctgctttt cggcagacaa
     5041 aaagcggcgc aactcatgca tataggagga caggaactcg ggctccagcg ccgccctcca
     5101 ctcgggattc atcctgacat gctgcaaagt gtcactcata ccgacaacat cgcccgcaac
     5161 tgatgggaga tagcctgcct ggccacatcg ggacaaaagg actcgtgcga gctcagcatg
     5221 ccctcaaact ccagccgacg gatcaaacac atcagggagc gtgccgcatc ctttacttcg
     5281 tgagcaccta gatacgccag aagacgggta aagttagcca tcagtcgctg atcatgctgg
     5341 atcgccaacg agtgcatgcg ggcgtctgtc agtgccgcat agcgaaaggc gttgtcgatg
     5401 atgatgttgt cccttagaga actcagtcgc aggaggacat attcagcccc catcccggac
     5461 aggtgcgcca ccacctcggc taactgctca ggatgttcac gcaccccttc gggataggca
     5521 ctcagccaga gattggccct gctccagaaa ggatcaatgt agctttccat ggactccttg
     5581 gcatacagcg tgaacgcatc cgccagcaag tcatggatgt ccttgaaata gtatgtggtc
     5641 gccgacaagg gtacccctgc ctccgccgcc accgcgcgat gtcgcacgcc ccttactccg
     5701 tcccgagcga tcactcggag tactgcccgc aaaatcgcca aacgacgctg ctcactccct
     5761 tcccgggagg ccttccgccc ctgaaaagca atctgtgctt tagtccccgg catcatgttg
     5821 tcccgttatt ccaatgaccg ccaaggatac tggcgtcggt cgcggctgcc catagctggc
     5881 aacgcttcgc agggatgcaa accagccgac agagaacgct gtttttatga caaacggcgc
     5941 ccgtagaaaa ctcttggatt ttaaacaaaa aaatcaatac gttagcgaca caaactgtcg
     6001 tcagcttgtg atttaaggac gaccattagt gacaattacg cattgacata tggacagaca
     6061 ggaaagactt acttacatca tccgtttatt gcaggacatg tccctcaatt ccatggaaat
     6121 caaggcacga tttcctggcg tatccccgcg cacgatcgaa agagaccttg aacaactggc
     6181 ccaggaaggg cgaatccatc aagtgcgccg tgatcgcagc cgaaacccta catgggaaac
     6241 cggggaacgg gcaccaacca tcgaagtacg ctggatgaac ggccgcacgg ctgcagccat
     6301 caaggtcatg gagagctgcc tggccaccat actgcccaac acattgctgc aagaccttcg
     6361 ccctctgttc agccgcgccg acatcgccct gaagcaggcc cacaatcgcg aatatgccaa
     6421 ctggctgcag aaaatccgga tcagaccgag cctcaaggag gacgagaacg ccgacgtgac
     6481 cagccgccat ctgggcatca ttcaggaagc actgctgaaa agccaggcca ttcacgtcac
     6541 cgaacgtccg gacagcatga cggccatcag ccgaaccatt catccacaag gcttgctgtt
     6601 ctcacggtcg ggggcacatc tgattgccac tgacgatgca gcacatgaca tacccttgca
     6661 gatctgcatc caggacattt cggaagtcga actgctcgat cagccctcaa atacaccggc
     6721 cggttttaat ctggatcact ggcttgatag ccgcaagaaa gaagaagcaa ctgagctgac
     6781 agcaagct
//
LOCUS       DASUEG010000411         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1498873, whole genome shotgun sequence.
ACCESSION   DASUEG010000411 DASUEG010000000
VERSION     DASUEG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498873"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="atpB"
                     /locus_tag="VFX11_07640"
     CDS             <1..709
                     /gene="atpB"
                     /locus_tag="VFX11_07640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX4938530.1"
                     /translation="TGEGFWTFNLDTLLMSSVLGLVLVLTFALAARRATSGVPGHLQN
                     LVESVIEFIDGVVHETFHGDSRLVAPLAMTIFCLVLLWNTIDLIPVDLFPAVAGLFGV
                     QYFRPVPSADMSATFGLSITVFLLIIIYSIKGKGVGGYIQELLFHPFGKYLMPFNLIL
                     NAVELLAKPLSLGLRLFGNLYAGELIFVLIALLPWWAQWGPGGAWAIFHILVIPLQAF
                     IFMTLTVVYLGMAHAKH"
     gene            751..1017
                     /gene="atpE"
                     /locus_tag="VFX11_07645"
     CDS             751..1017
                     /gene="atpE"
                     /locus_tag="VFX11_07645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX4938531.1"
                     /translation="MEALIAQIQANTVLTVGIIFGLAAVGTAIGFALLGGKFLEGVAR
                     QPELAGMLQTRMFIIAGLLDAVSIIGVAMGLLMMFANPFLAALG"
     gene            1075..1545
                     /locus_tag="VFX11_07650"
     CDS             1075..1545
                     /locus_tag="VFX11_07650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX4938532.1"
                     /translation="MGINATLIGQMITFGLFVWVMMKFVWPPLTQAMQERQQRIAEGL
                     AAAEQGNTQLERSRQEVESALQAARQQAAEILAQANRRGAEMIEQAKQAAREEGERQI
                     RSAEALIAQEVSQARESLRREVADLAMAGAGRILKREIDPSAHRDLVDELVGQI"
     gene            1560..2099
                     /locus_tag="VFX11_07655"
     CDS             1560..2099
                     /locus_tag="VFX11_07655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX4938533.1"
                     /translation="MSEVTTLARPYAKAVLDLAQQSGAALSEWQQRLDLLAALVSYPP
                     IRTAIDNPLQTAEQSRQLIIDALGPELGPEGINLASLLIENGRLLLTPQIAELYGAMR
                     AEAEGSVDAEVTTATPMDADQQQRISEALSRRLGRRVNLTSRVDASLVGGAVIRAGDL
                     VIDGSVRSQLERLSRALVH"
BASE COUNT          396 a          625 c          673 g          476 t
ORIGIN      
        1 gaccggggaa ggtttctgga ccttcaacct ggataccctg ttgatgtcga gcgtgctcgg
       61 cctagtgctg gtgctgacat ttgcgctggc cgcacgtcgg gctacctccg gggttccggg
      121 gcatctgcag aacctggtcg agtccgtcat tgaatttatc gatggcgtgg ttcatgagac
      181 cttccatggc gacagccgtc tggttgcgcc gctggccatg accattttct gcctggtttt
      241 gctctggaac acgattgacc tcatcccggt ggatttgttc cccgcggtgg ccggcttgtt
      301 cggcgttcag tatttccggc ctgtcccatc ggcggacatg agtgccacct ttggcctgtc
      361 catcacggta ttcctgttga tcatcatcta cagcatcaag ggcaagggcg tcggcgggta
      421 tatccaggaa ctgttgttcc atcctttcgg taaatacctg atgccgttca acctgatcct
      481 gaacgcggtc gagttgctgg ccaagcccct gtcgctgggc ctgcgactgt tcggcaacct
      541 gtatgccggc gaattgattt ttgttttgat cgccctgctg ccctggtggg cgcagtgggg
      601 gcccggtggc gcctgggcaa tcttccatat cttggtcatt ccgctgcagg cgttcatttt
      661 catgaccctg accgtggtct acctgggtat ggcacacgcc aagcactgat ctctttggct
      721 cacactgata ttcccgaacg aggaggaatg atggaagctc ttatcgccca gatccaggcc
      781 aataccgtgt tgacggttgg tatcattttt ggtcttgccg ccgtcggcac cgccatcggt
      841 ttcgccctgc tgggtggaaa attcctggaa ggtgtggccc gtcagcccga gctggctggc
      901 atgctccaga cccgtatgtt catcatcgcc ggtcttctgg acgctgtgtc cattatcggt
      961 gtggccatgg gcctgttgat gatgttcgcc aatcccttcc tggcagcgct gggctgaaac
     1021 gtcccctcga cccggacgtt ttttgggtag gacacggatc gcggagagtt tatcgtgggc
     1081 atcaacgcca ctctgatcgg ccagatgatc accttcgggt tgttcgtctg ggtcatgatg
     1141 aagttcgtct ggccgccgtt gactcaggcg atgcaggaac gtcagcagcg catcgctgaa
     1201 ggactggctg ccgccgagca ggggaacacc cagctggagc gttcgcgcca ggaagtcgaa
     1261 agcgccttgc aggcggcacg ccagcaggca gccgaaatcc tggctcaggc gaatcgccgt
     1321 ggcgccgaga tgatcgagca ggccaagcag gcggcgcggg aggaaggtga gcggcagatt
     1381 cgctctgccg aagccctgat cgcccaggaa gtgtcccagg cccgggaatc cctgcgacgg
     1441 gaggttgcgg atctggccat ggctggggca ggacggatac tcaagcgtga aatcgacccg
     1501 tccgcccacc gtgatctggt ggacgaactg gtcggccaga tctgaccggg aatccacaga
     1561 tgtccgaagt aacgaccctg gcgcgaccct atgcgaaagc cgtgcttgat ctggcccagc
     1621 aaagtggagc ggcgctgagc gaatggcaac agcgattgga tctgttggcg gccttggtgt
     1681 cctatccacc catccgcacg gcgattgaca atccgctgca gactgccgaa cagagccgcc
     1741 aattgatcat tgacgccctg ggccccgaat tggggccgga aggcatcaat ctggccagtc
     1801 tcctgataga aaacgggcgt cttttgttga cgccacagat tgccgagctt tatggcgcaa
     1861 tgcgcgcaga ggctgaaggc tcggtggatg ccgaagtgac aacagcaacc ccgatggatg
     1921 ccgatcagca acagcggatc agcgaggcct tgtcgcggcg gctcgggcga cgggtcaacc
     1981 tgacctcccg ggtcgacgca agcttggtgg gcggcgcggt cattcgcgcc ggtgatttgg
     2041 ttatcgacgg gtcggtccgc agccagctgg aacgtctttc ccgcgcactg gttcattgac
     2101 gaggaagcaa catgcaagtc aatcccgccg agattagcga gctgatcaaa cagcgcatcc
     2161 agaacttcca
//
LOCUS       DASUEG010000412         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_90005, whole genome shotgun sequence.
ACCESSION   DASUEG010000412 DASUEG010000000
VERSION     DASUEG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90005"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFX11_07660"
     CDS             <1..892
                     /locus_tag="VFX11_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4938534.1"
                     /translation="RIIGNLVEQVADKAAAGKVIDGVKEFSIPLTIAVICEQLGMNQY
                     DGDKISEWSAAITAQIGRMQNREQMIENAKKVCELQNFIIGQMKDREANPKEDMISDL
                     VHAHTEDGGKLTFGEAVSLVRALIIAGNDTTATAISNLLFILATQPAVATQLYDNVED
                     ERYLTRFIEELLRYAPPVRGLAKMTTRETELGGTKLPKGAHMLVLYGSGNDDERRFEC
                     PRTFDAARGNVGNHVAFGVGIHRCIGASLARMEIRVAAKELIKRVRDIRLAIPIDQVA
                     YLPTVATHSISSLPITLMRR"
     gene            997..1845
                     /locus_tag="VFX11_07665"
     CDS             997..1845
                     /locus_tag="VFX11_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938535.1"
                     /translation="MGQELAGKVAIVTGGAGGIGRATVELFVREGARVVIADINDEQG
                     EELAKELGKAAFYQRVDVSRAEQMQALVDTAVEQFGGLDIMFNNAGISGAQYPRFLDD
                     DLADFDRVVGINLFGVMSGSRSAGKYMSKHGGGVIINNASIAGLLPGQALMTYRATKA
                     AVIMFSKSLAIDLAEYGIRVNCLAPGHIRTGLTAFSMPGMSAEQIERVKQAIAPAFDS
                     NQPLKRHGKPEDVAQTVLFLASNRAAQITGVVLPVDGGITAGDPVNHLREIMAARAQA
                     LAEIQA"
     gene            1888..2220
                     /locus_tag="VFX11_07670"
     CDS             1888..2220
                     /locus_tag="VFX11_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4938536.1"
                     /translation="MSVVKITFVERSGAEKVVEDAEIGDNMMQVATANGIDGILGECG
                     GGCACGTCHIYIDPEWQDVVGAADDVEDMTLDMVSETRKPTSRLCCQIKVSEAMDGLR
                     VVVAPEAL"
BASE COUNT          574 a          643 c          725 g          467 t
ORIGIN      
        1 ccgcatcatt ggcaacctgg tggagcaagt tgctgacaaa gcggccgccg gcaaagtaat
       61 cgatggcgtg aaggaatttt ccattccatt gaccattgcc gtcatttgcg aacagctggg
      121 catgaaccag tacgatggcg acaagatatc ggaatggtct gccgccatca cggcgcaaat
      181 cggaaggatg cagaatcgcg aacagatgat tgagaacgct aaaaaggttt gcgaactgca
      241 gaatttcatt atcggccaga tgaaggaccg cgaagccaac cccaaggaag acatgatttc
      301 cgatctggtg catgcccaca ccgaagatgg cggcaaactc accttcggcg aggcagtatc
      361 cctggtgcgt gcgttgatca tcgccggcaa tgacaccaca gccacggcca tcagcaattt
      421 gttgttcatc ctggcaacgc aacccgcagt ggcaacacag ctctatgaca acgtggaaga
      481 cgagcgctac ctgacccgtt tcatagagga gctgctgcgc tacgccccac cggttcgcgg
      541 ccttgccaaa atgacaacca gggaaacgga gctgggcggg acaaaactgc ccaagggcgc
      601 ccacatgctg gtgttgtacg gctccggcaa tgacgatgaa cggcgctttg agtgcccccg
      661 cacgttcgat gcagcacgcg gcaacgtcgg taaccatgtg gcgttcggtg taggcatcca
      721 tcgttgcatt ggggcatccc ttgcccgcat ggaaatcagg gtagcggcaa aggaactgat
      781 caaacgcgtc cgcgatatca ggttggcgat accgatcgac caggtggcat atctgcctac
      841 tgtagccacg cattcgattt cgtcgcttcc cataacgctg atgcgccgct aaatcgacag
      901 gcaataatgg ttgcccaagg cgcgcagttt cgcgcctttt tgcacaatgg ccaacaaggc
      961 cgccggtatc atcatagcgc cgggctggag gtttgagtgg gacaggaatt ggcaggtaag
     1021 gtcgccatcg ttaccggtgg ggctggagga atcgggcgtg caaccgtgga attgttcgtg
     1081 cgcgaaggtg ccagggtggt catcgccgat atcaacgatg aacagggtga agaactcgct
     1141 aaggaactcg gcaaagccgc gttctatcag cgcgttgacg tatccagggc cgagcagatg
     1201 caagcgctgg tggataccgc agtggagcag tttggcgggc tggacatcat gttcaacaat
     1261 gcgggcattt ccggcgcgca gtacccccgc ttcctagatg atgatctggc agacttcgat
     1321 cgcgtcgttg gcatcaacct gtttggcgta atgtccggca gccgcagtgc gggcaagtac
     1381 atgtcaaaac acggcggtgg cgtcatcatc aacaatgcct ccattgccgg attgctgccc
     1441 gggcaggcac tgatgacata ccgcgccacc aaagcagccg tgatcatgtt cagcaagtca
     1501 ctggcgatcg atctggcgga atacggaatt cgtgtgaact gtctggcgcc cggtcatatt
     1561 cgcacgggcc tgaccgcttt ttccatgccg ggaatgagcg cggagcaaat cgagcgcgtc
     1621 aagcaggcaa tagcaccagc gtttgattca aaccagccac tcaaacggca cggcaagccg
     1681 gaagacgtgg cacaaacggt gttgttcctc gccagcaacc gggcggcaca gatcacaggt
     1741 gttgtgctgc cggtggatgg cggcatcacc gccggagacc ctgtcaatca cttgcgggaa
     1801 atcatggcag cacgcgccca ggcgctggca gaaatacagg cttgaaatcg caaaaccagc
     1861 gatacggtac atgaggagta gtgggtaatg agcgtagtaa aaatcacctt tgttgaacgc
     1921 agcggcgccg aaaaagtggt ggaggatgcc gaaatcggcg acaacatgat gcaggtggcc
     1981 accgccaacg gtatcgacgg tattctgggc gaatgtggcg gcggttgtgc ctgtggtacc
     2041 tgccacatct acatcgatcc ggagtggcag gatgtagttg gcgcggccga tgatgtagag
     2101 gacatgacgc tggatatggt gtccgagaca cgcaaaccca ccagccggct gtgctgccag
     2161 atcaaagtca gtgaagcaat ggacggattg cgggtagtcg tggcgccgga agcactttga
     2221 cagctccatc gactgaacgt acgcaggccc gccctgctgc cgattcaata tcagattaaa
     2281 gtgaagtcat cctcgccttt tcagattacg gagaataaaa atgagtgcaa cagaagccta
     2341 tgacgttgtt gtcgtgggtt ccggcgccgc tggagcagtg gccgcactgc gtgctgcaga
     2401 tctcggact
//
LOCUS       DASUEG010000413         5595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_509432, whole genome shotgun sequence.
ACCESSION   DASUEG010000413 DASUEG010000000
VERSION     DASUEG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509432"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX11_07675"
     CDS             complement(<1..452)
                     /locus_tag="VFX11_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX4938537.1"
                     /translation="MHLHFLGICGTFMGSLAVLAKALGHQVSGSDANVYPPMSTQLEE
                     QGIKLVEGYDPAQLDPAPDLVIVGNAMSRGNPAVEYMLDRGLPYTSGPQWLHDHVLQG
                     RWVIGVAGTHGKTTTTTMVTWILDQAGLEPGYLIGGVPKNFDVSARLG"
     gene            complement(512..1132)
                     /locus_tag="VFX11_07680"
     CDS             complement(512..1132)
                     /locus_tag="VFX11_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938538.1"
                     /translation="MSALVPVAGIWHNDGRFEVKEPALKPLFTLFWQICRFRSGPEDV
                     PYAPALLGLMLVLVLTLGLSSIPLLSAYDDPAKAVPAAQQVIALLFSVSSWMVILYAL
                     LRFMNHGARYVQTMTAALGTDLIFGVPQVVLYWLLLSSQAQSALAMLAQSVLLGVYIW
                     DMLVKGAIYSRALGIGRLQGNLLSLALSFGLFMLSSLLFLPPPPSP"
     gene            1222..2487
                     /locus_tag="VFX11_07685"
     CDS             1222..2487
                     /locus_tag="VFX11_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX4938539.1"
                     /translation="MAKRNAFYAQSGGVTAVINASACGVIETARQFPDRIGKLYAGHN
                     GIVGALTEDMIDTSCESAATIAALKYTPGGAFGSCRYKLRSFEENRREYERLVEVFKA
                     HDIGYFFYNGGGDSQDTAHKVSQISERMGYPITCIGIPKTVDNDLPFTDCCPGFGSVA
                     KYVAISTREAALDVASMCETSTKIFILEVMGRHAGWIAAAGALAREDAGDPPHVILFP
                     EIKFDREAFLRKVKASVKDHGFCVIVASEGAQNPDGTFLADSGGKDAFGHTQLGGVAP
                     YLAHMIKSELGYKYHWALADYLQRAARHIASATDVDQAYAVGRAAVEFALAGKNAIMP
                     TIVRNPGPEYSWSIGEAPLSQVANVEQKMPRDFISEDGFDITAPCLDYLRPLIQGEAY
                     PPYKNGIPEIVHLKKVKVEKVLNTPFSVK"
     gene            2518..3312
                     /locus_tag="VFX11_07690"
     CDS             2518..3312
                     /locus_tag="VFX11_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HEX4938540.1"
                     /translation="MRGIEETWTSIKHYFGGHVVHRSRDEWGDILVVDNRHLRVMSFG
                     SAFEQSCIDVRTPHVPVHEYARAMLLSLAFTTPRHMTLLGLGGGALVSSLRHALPDCP
                     LQVVELREEVAQVAQDYFGLIPNEHLMIQIEDARQYLRNAEPASTDLLFSDMYYSADM
                     NPFQRQKKFIQHCQRVLTDDGWLVINYHRLPDGDDPFFNWMRQSFPTLYTCATGSGNQ
                     VVLASKGPCPDVATVREKTGELEIKLGARLMPLLLKLEKLGEPKDK"
     gene            complement(3448..4188)
                     /locus_tag="VFX11_07695"
     CDS             complement(3448..4188)
                     /locus_tag="VFX11_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX4938541.1"
                     /translation="MQRLVWWIQWHRRIGAVVAVLVIFLAVTGVLINHSQALGWHRAP
                     VHSATLAKLYQIPLQDLEQGFALDSRWIVQRGEQVLLDRQPVAECSAGLTGAALYQDM
                     IAVLCVDELLLLDPQGQPVETLRGLPQPGRALALQDNALWVRGDSAIFGFDDAAGVWQ
                     PVAPEHTPQWILPQPLPTPLKTALNADNPVPGLTRERVLLDLHSGRLFGTAGVWVVDA
                     VGVLICLLAFSGLLTWVGRYYRRLRRRH"
     gene            complement(4232..4789)
                     /locus_tag="VFX11_07700"
     CDS             complement(4232..4789)
                     /locus_tag="VFX11_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX4938542.1"
                     /translation="MVRKAVLLLVLAGWCTGISLVVQAMGIPQIQYLTQEQFVQQTFG
                     SSQPQWKMLILDATLKQKAAEILQHPYTGMRVRYWIAGQRTAWIIDEIGKEMPITIGV
                     AVDGDSIQQVKILVYREERGGEVHQDFFTRQFESVTLTGDERLSRSIDGITGATLSVQ
                     AVSRVANLVLVMHRHVLAKAAAQGG"
     gene            complement(4792..>5595)
                     /locus_tag="VFX11_07705"
     CDS             complement(4792..>5595)
                     /locus_tag="VFX11_07705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4938543.1"
                     /translation="GRALLLQTAHPWGTAGIDEHSIVRYDPMERFRRTFSNISTMVYG
                     SMPQVLNAANKVRTSHKEIKGKIPYRAGAFHKDSAYGANEVNAMVWVHATLWETLVYM
                     YEELEEPLTQAEKDRFYEETKLFAMLFGIPESALPRNWNEFMAYNEAMWYSSQLTVTD
                     NARKLKEDLFTPRSVWLIFPLWIQEVVTASNIPAPIREGYQMDYGLWERFNNAWLMGS
                     AEFTSWILPDYLGVNPVDHEAHARLEGRRNDYFHRKQIEAVMGTDRLVN"
BASE COUNT         1149 a         1819 c         1614 g         1013 t
ORIGIN      
        1 ttgcccaggc gggcggacac atcgaaattc ttcggcacgc cgccgatcag gtagcctggc
       61 tccagcccgg cctgatccag aatccaggtc accatggtgg tagtggtggt cttgccgtgg
      121 gtgccggcca cgccgatcac ccagcgcccc tgcagcacat ggtcgtgcag ccattgcggg
      181 ccagaggtat aaggcaggcc gcggtccagc atgtattcca ccgccggatt gccccgcgac
      241 atggcgttgc cgacgatcac cagatccggc gccgggtcca gctgcgcggg gtcgtagcct
      301 tccaccagtt tgatgccctg ctcttccagc tgggtgctca tgggcgggta gacattggcg
      361 tcggaaccgc tgacctgatg gcccaacgcc ttggccagca ccgccaggct gcccatgaag
      421 gtgccacaga ttccgagaaa gtgcagatgc atgcgcgctc cttgattcga ggatgaggag
      481 tggaggaaaa gcctgccggc cagggcgacg gttacggcga cggcggaggc ggcaggaaca
      541 gcagcgaact gagcatgaac aggccgaacg acagcgccag cgacagcagg ttgccctgca
      601 ggcggccaat gcccagggcc cggctgtaga tcgcgccctt caccagcata tcccagatat
      661 acacccccag cagtaccgac tgggccagca tcgccagtgc cgactgcgcc tggctggaca
      721 gcagcagcca gtacagcacc acctggggca cgccaaagat cagatcggtg cccagcgcgg
      781 cggtcatggt ctgcacatag cgggcaccgt ggttcatgaa ccgcagcagg gcatacagga
      841 tgaccatcca ggaactgaca ctgaacagca gggcaatcac ctgctgtgcc gccggcacgg
      901 ccttggccgg atcgtcgtag gccgacagca gcggaatgct cgacagcccc agcgtcagca
      961 ccagaaccag catcagcccc agcagggcgg gggcataggg aacatcctcg gggccgctgc
     1021 gaaagcggca gatctgccag aacagggtga acagcggctt caacgcgggc tccttgactt
     1081 caaatcgccc gtcattgtgc caaatccccg ccaccggcac caatgccgac atgaaaaagg
     1141 ccgccttttc ctttatcatg ccgcctgatt tcggggccgt gcggcccgta accgattccg
     1201 cgtcctgggg gaatgttccg aatggcgaaa cgtaacgcgt tctatgcgca gtccggtggc
     1261 gtgactgcgg tgatcaatgc cagcgcctgt ggcgttatcg agaccgcccg ccagttcccg
     1321 gatcgcatcg gcaagctgta cgccggccac aacggcatcg tcggcgccct taccgaagac
     1381 atgatcgaca cctcctgcga atccgccgcc accatcgccg cactgaaata cacgcccggc
     1441 ggcgccttcg gctcctgccg ttacaaactg cgctcattcg aggaaaaccg ccgcgaatac
     1501 gaacgcctgg tggaagtgtt caaagcccac gacatcggct acttcttcta taacggcggt
     1561 ggcgattccc aggacacggc ccacaaggtt tcgcagatca gcgaacgcat gggctacccc
     1621 atcacctgca tcggcatccc caaaaccgtc gacaacgacc tgcccttcac tgattgctgc
     1681 cccggcttcg gttccgtcgc caagtacgtt gccatatcga cgcgtgaagc agcactggac
     1741 gtggcgtcca tgtgcgaaac gtccaccaag attttcattc tggaagtgat gggccgtcat
     1801 gccggctgga tcgccgcggc cggcgcgctg gcgcgggagg acgcgggcga tccgccccat
     1861 gtcatcctgt tcccggaaat caagttcgac agggaagcct tcctgcgcaa ggtgaaagcc
     1921 agcgtgaagg atcacggctt ctgcgtgatc gtggcatccg agggcgcgca gaatcctgac
     1981 ggtaccttcc tggcggattc cggcggcaag gacgcgttcg gccacaccca gctgggtggc
     2041 gtggccccct atctggcaca catgatcaag agcgaactgg gctacaagta ccactgggca
     2101 ctggccgatt atctgcagcg ggccgcgcgc catatcgcat cggccaccga cgtggaccag
     2161 gcctatgccg tgggcagggc ggcggtggaa ttcgcgctgg ccggcaagaa cgccatcatg
     2221 cccaccatcg tgcgcaaccc cggcccggag tactcctggt ccattggcga ggcaccgctg
     2281 tcgcaggtgg ccaatgtgga acagaagatg ccccgcgatt tcatctccga agacggcttc
     2341 gacatcaccg ccccctgcct ggactacctg cgtccgctga tacagggcga agcctacccg
     2401 ccgtacaaaa acggcattcc cgaaatcgtg catctcaaaa aggtgaaggt ggaaaaagtc
     2461 ctcaataccc ccttcagcgt gaaatgatga cggtgccggg ctcctcccgg gaatgacatg
     2521 cgcggaatcg aagaaacctg gaccagcatc aaacactatt tcggcggcca tgtagtgcac
     2581 cgcagccgcg atgaatgggg cgacatcctg gtggtggaca accgccacct gcgggtgatg
     2641 tcgttcggct ccgccttcga gcagagctgt atcgacgtgc gcacccccca cgtacccgtg
     2701 catgaatacg cgcgcgccat gctgctgtca ctggcgttca ccacgccgcg ccacatgaca
     2761 ctgctgggcc tgggcggtgg tgctctggta agcagcctgc gacacgcgct gccggactgc
     2821 ccgttgcagg tggtggaact gcgggaggaa gtggcgcagg tggcgcagga ctatttcggc
     2881 ctgataccca acgagcacct gatgatccag atcgaggatg cccgccagta cctgcgcaat
     2941 gccgaaccgg ccagcaccga tctgctgttt tccgacatgt attattccgc cgacatgaac
     3001 ccgttccagc gccagaagaa attcatccag cattgccagc gcgtgctgac ggatgacggc
     3061 tggctggtga tcaactacca ccgactgccc gatggcgacg atccgttttt caactggatg
     3121 cgacaatcgt ttcccactct gtacacctgc gccaccggca gcggcaatca ggtggtgctg
     3181 gcgagcaaag gcccatgccc ggatgtggcg acggtgcggg agaaaaccgg tgaactggaa
     3241 ataaagctgg gcgcgcggct gatgccgttg ctgctgaagc tggaaaagct gggggaaccc
     3301 aaagacaaat aaaaaaaatc aggcggacga aagactggaa ttgtccgcct gattgttcaa
     3361 acacccttca aatctgaacc aaacacctcg cttgtgtaat cggctttcgt tgaatttcct
     3421 gcatcgcagc gcccgtcacc gcatgactca gtgccggcgc cgcaaacggc ggtaataacg
     3481 acccacccag gtgagcagac cactgaacgc cagcagacag atcagcacac ccacggcatc
     3541 caccacccag acacccgccg tcccgaacag gcgaccgctg tgcagatcca gcagcactcg
     3601 ctcccgcgta agcccgggca ccggattgtc ggcattgagc gccgtcttca acggtgtcgg
     3661 caatggctgc ggcaaaatcc actgcggcgt gtgttccggt gccaccggtt gccagacacc
     3721 ggcggcgtca tcgaaaccga agatcgcgct gtcgccccgc acccacagtg cattgtcctg
     3781 caacgccagc gcgcggcccg gctgcggcaa tccgcgcagg gtttccaccg gttgcccctg
     3841 cgggtccagc agcagcagtt catccacgca cagcacggcg atcatgtcct gatacagcgc
     3901 tgcgcccgtc agacccgcac tgcattccgc taccggctgc cggtccagca gcacctgttc
     3961 gccacgctgc acgatccagc gggaatccag cgcgaaaccc tgctccagat cctgcagtgg
     4021 aatctggtac agcttcgcca gcgttgcaga atgcaccgga gcgcggtgcc agcccaacgc
     4081 ctggctgtga ttgatcagca cgcccgtgac ggccagaaaa atcaccagca ccgccaccac
     4141 tgcgccgatg cgacgatgcc actgaatcca ccacaccaga cgctgcatac ccgtacttcc
     4201 caccactgca attaggttgt tcgtgctgcc gctacccgcc ctgcgcggcg gcctttgcca
     4261 gaacatgccg gtgcatcacc agcaccaggt tggccacccg cgacacggcc tgtaccgata
     4321 gcgtcgcgcc cgtgatgccg tcgatgctgc gggacagacg ctcatccccc gtcagcgtga
     4381 cactttcaaa ctggcgcgtg aaaaagtcct gatgcacttc gccaccacgc tcttcgcgat
     4441 acaccagtat cttcacctgc tggatgctgt caccgtccac tgccacaccg atggtgatcg
     4501 gcatttcctt gccaatctcg tcgatgatcc aggcggtccg ctgcccggca atccagtaac
     4561 gcacccgcat gccggtgtag ggatgctgca ggatttctgc ggccttctgt ttcagggtcg
     4621 catccagaat cagcattttc cactgcggct ggctgctgcc aaacgtctgc tgtacaaatt
     4681 gttcctgggt caggtactgg atctggggaa ttcccatggc ttgcaccacc agggaaattc
     4741 ccgtacacca gcctgccagt accagcagca gaactgcctt gcgtaccata ttcaattcac
     4801 cagtcgatcc gtacccatca cggcttcaat ctgcttgcga tggaaataat cattgcgtct
     4861 gccttccagc cgggcatgcg cctcatgatc caccggattg acgcccagat aatccggcag
     4921 gatccagctg gtaaattccg ccgagcccat cagccaggca ttgttgaagc gttcccacag
     4981 gccgtaatcc atctgatagc cttcgcggat tggtgccgga atgttggagg ctgtcaccac
     5041 ttcctggatc cagagcggga aaatcagcca cacggaacgc ggcgtgaaca ggtcttcctt
     5101 cagcttgcgg gcgttgtcgg tcacggtcag ctgcgagctg taccacatgg cttcgttgta
     5161 ggccatgaat tcattccagt tgcgcggcag tgcgctttcc ggaatgccga acagcatggc
     5221 gaacagtttg gtttcctcgt agaagcgatc cttttccgcc tgcgtcagtg gctcttccag
     5281 ctcttcgtac atgtacacca gggtttccca caaggtggcg tgcacccaga ccatggcgtt
     5341 cacttcgttg gcgccgtacg cagaatcttt atggaaggca ccggcgcgat agggaatctt
     5401 gcctttgatt tccttgtgcg acgtgcgcac cttgttggcc gcgttcagca cctgcggcat
     5461 gctgccgtac accatggtgg aaatattgct gaaggtgcgg cggaagcgct ccatcggatc
     5521 gtagcgcacg atggaatgct catcgatgcc ggcagtcccc cagggatgtg ccgtctgcaa
     5581 cagcagcgcg cggcc
//
LOCUS       DASUEG010000414         8088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_681401, whole genome shotgun sequence.
ACCESSION   DASUEG010000414 DASUEG010000000
VERSION     DASUEG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681401"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFX11_07710"
     CDS             complement(<1..246)
                     /locus_tag="VFX11_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX4938544.1"
                     /translation="MAVGKETFGTLHPVKGFRLGTTQAGVKKADRRDLVVMEAAAGSS
                     IAGVFTLNAFCAAPVHICKQHLQQIEGFCEQTTYLITN"
     gene            complement(336..3056)
                     /gene="secA"
                     /locus_tag="VFX11_07715"
     CDS             complement(336..3056)
                     /gene="secA"
                     /locus_tag="VFX11_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX4938545.1"
                     /translation="MFGPLLRKIFGSKNERELKRMGKLVVRINELETSLQTLSDEALK
                     GKTTEFRQRLEKGEQLNDLLPEAFAVVREASRRVMGMRHFDAQMIGGITLHEGRIAEM
                     RTGEGKTLTATLPAYLNALPGQGVHVVTVNDYLAQRDANWMRPLYEFLGLTVGVILSQ
                     QPPDLKRAAYASDITYGTNNEFGFDYLRDNMAFTLQDRVQGPLAYAIVDEVDSILIDE
                     ARTPLIISGPSEDSSELYRRINLLIPRLKRGTVEGEETTGHFTVDEKNRQVELSEEGH
                     VYIEELLTENKLLGEGDSLYAANNLSLLHHVHAALKAHVLFHRDVEYIVQNGQIVIVD
                     EHTGRTMPGRRWSDGIHQAIEAKEGVKIQSENQTLASTTFQNYFRLYRKLSGMTGTAD
                     TEAFEFHQIYGLDVVVIPTNRPMIRQDHNDLIYMSAEEKYEAIIEDIKGCIEAGRPAL
                     VGTASIETSEYLSNLLKKQKIAHKVLNAKFHAQEAEIIAQAGQPGAVTIATNMAGRGT
                     DILLGGNWQVEVQALDPATPEKIAAIKAAWEERHAQVLAAGGLHIIGTERHESRRIDN
                     QLRGRSGRQGDPGSSRFYLSLEDNLMRIFASDRVKGLMQTLGMERGEAIEHRWVTRSI
                     ENAQKKVEGRNFDIRKSLLEYDDVANDQRRVVYEQRNDILNADDISENIAAIRADVVN
                     EVITGYIPPQSMEEQWDLEGLERALETDFRAHMPIRQWMAEDKNLHEEPLRKKILQTL
                     EEAYAAKEAALGDEKQVMRQLEKHVMLATVDRLWKEHLANMDHLRQGIHLRGYAQKNP
                     KQEYKREAFELFQTFLENVKLEVIRILSTVELRRQEEVEELERQRREAAERDIKLQHA
                     SASGLQEEKPEEGDDESLAPFQRTERKVGRNEPCPCGSGKKYKHCHGQVE"
     gene            complement(3243..4160)
                     /locus_tag="VFX11_07720"
     CDS             complement(3243..4160)
                     /locus_tag="VFX11_07720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4938546.1"
                     /translation="MNIILFDSRTGHSRTLRLRAGFMAGIALTLLLATGLTGAMAGIW
                     WSDRGIELTAAALLQRDAAAQAEEVGRARLNAEEQLTAMTVRMAELQARLLRLDALGE
                     RLVGMARLDGGEFDFSRPPPVGGPEHALDAAYERPGFLAELDRLADYIGKREQQLEVL
                     ESLLANRKLQEQADLTGHPLEHGWLSSPFGRRSDPFTGRLAMHEGVDLAGKAGADFRS
                     VAAGVVTFAGSRKGYGKFVEVNHGGGYVTRYAHARDLDVKVGDIVKKGQTLGKIGSSG
                     RSTGPHVHFEVRLNGKVVNPAHYLNLKQG"
     gene            4260..4688
                     /locus_tag="VFX11_07725"
     CDS             4260..4688
                     /locus_tag="VFX11_07725"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX4938547.1"
                     /translation="MQELLRHNRGVKNLTDKAGKIQELQRALTLVLPTMLADYCQARR
                     FDGGVLYLDAATGSVATQLRFIQSQLVQKLQKIAVFSTLDRISIRVQGPAQAVRKPGK
                     RKTPPVSAENCQLLEEAANNISDTALAESLRTLAKTLEGL"
     gene            complement(4753..5667)
                     /gene="lpxC"
                     /locus_tag="VFX11_07730"
     CDS             complement(4753..5667)
                     /gene="lpxC"
                     /locus_tag="VFX11_07730"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX4938548.1"
                     /translation="MIRQRTLKNVIRATGIGLHSGDKVYLTLRPAPANTGIVFCRTDL
                     DPVVTIEAKAFSVANTVLSTNLVKGDVRVSTVEHLMAAFAGLGIDNAYVDLSAPEVPI
                     MDGSAGPFVFLLQSAGIEEQNALKQFIRIKRKVQVTDGDKSATFLPFNGFKVGFTIDF
                     DHPVIKGGNQTISIDFSSTSFVREVSRARTFGFMRDIEKLRKNNLALGGSTENAIVVD
                     DYRILNEDGLRYEDEFVRHKVLDAIGDLYLMGKSLIGEFCGYKSGHALNNKLVRELLS
                     QQDAFEIVTFGDQKVSPISYVQPIFAAE"
     gene            complement(5785..6957)
                     /gene="ftsZ"
                     /locus_tag="VFX11_07735"
     CDS             complement(5785..6957)
                     /gene="ftsZ"
                     /locus_tag="VFX11_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX4938549.1"
                     /translation="MFELVDNVPQNAVIKVVGVGGGGGNAVEHMLSSQVDGVEFICAN
                     TDAQALKNMNAKTLLQLGGAVTKGLGAGANPNVGREAALEDRERIREVLNGADMVFIT
                     AGMGGGTGTGGAPIVAEIAKEMGILTVAVVTKPFPFEGKKRMQVALEGMKHLSENVDS
                     LITIPNEKLLDVLGPKTSLLDAFKAANDVLLGAVQGIADLIIRPGMINVDFADVRTVM
                     SEMGMAMMGTGRATGENRAREAAEAAIRSPLLEDVNLQGARGILVNITASESLSLGEF
                     SEVGDTVEEFASDNATVVVGTVIDPSLGDELRVTVVATGLGMGAVSEQAAPVRAVESV
                     TRRSAPEPVTDYRRFEQPAVDRARRQQQEVRRRAVLETDVNREYLDIPTFLRRQAD"
     gene            complement(7006..>8088)
                     /gene="ftsA"
                     /locus_tag="VFX11_07740"
     CDS             complement(7006..>8088)
                     /gene="ftsA"
                     /locus_tag="VFX11_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4938550.1"
                     /translation="DIESTVQSIQRAIEEAELMAGCQIHSVYVGIAGSHIRSLNSHGI
                     VAIRDREVNRADLERVIDAAQAVPIPADQKVLHILPQEYIIDSQEGIKEPLGMSGVRL
                     EAKVHLVTCAVNASQNIERCVRRCGLEVEDIILEQLASAYAVLTDDEKELGVCMVDIG
                     GGTTDIAVFTEGSIRHTAVIPIAGDQVTNDIAMALRTPTENANDIKIKYACALTQLAG
                     PDESIKVASVGDRPPRELSRQALAEVVEPRYDELFSLVQAELRRSGYEDMIAAGIVLT
                     GGSARMEGAVDLAEEIFHMPVRLATPQGISGLTEMVKNPIYSTGVGLLLYGQKQIEAG
                     AAQQKGQPVPQAGFMDRMKTWIKGNF"
BASE COUNT         1593 a         2444 c         2252 g         1799 t
ORIGIN      
        1 gttggtgatc agataggttg tctgttcgca gaagccctcg atctgctgca gatgctgctt
       61 gcagatatgc accggagcag cacaaaacgc attcagcgtg aacacgccgg caatggaact
      121 gcccgccgcc gcttccatca ccaccagatc acgtcggtcc gcctttttca cacctgcctg
      181 ggtagtgccc agccgaaagc ccttcaccgg atgcagtgtg ccaaaggttt cttttccaac
      241 cgccatgatc gatctccttt tataccgatt tatcagaaaa acgctggatg aaaaaagggg
      301 cttctgcaag ccccttgtgt aagtccgtgc tgtcgttact cgacctgtcc atgacagtgc
      361 ttgtacttct tgccggaacc gcaggggcag ggctcgttgc gacccacctt gcgctccgtt
      421 cgctggaacg gcgccaggct ttcgtcatcg ccttcctcag gcttttcttc ctgcaggcca
      481 gacgcgctag cgtgctgcag tttgatatcg cgctccgctg cctcgcgccg ttgccgttcc
      541 agctcttcca cttcctcctg ccgccgcaat tccacggtgg acagaatgcg gatcacttcc
      601 agcttcacgt tttccaggaa cgtctggaac agttcgaacg cctcgcgctt gtattcctgc
      661 ttgggattct tctgcgcata gccacgcagg tgaataccct gacgcaggtg atccatattg
      721 gccagatgct ctttccacaa ccggtccacc gtggccagca tcacatgttt ttccagctgt
      781 cgcatgacct gcttttcgtc gcccagcgcc gcttccttgg ctgcataggc ttcttcgagc
      841 gtctgcagga ttttcttgcg cagcggctcc tcgtgcagat tcttgtcttc ggccatccac
      901 tgccggatcg gcatatgggc gcggaaatcg gtttccagcg cgcgctccag gccttccaga
      961 tcccactgtt cttccatact ctgcggcgga atatagccgg tgatgacttc gttcaccacg
     1021 tcggcacgga tagcggcgat gttttcggaa atgtcgtccg cattcaggat gtcattgcgc
     1081 tgttcgtaca ccacgcggcg ctggtcattg gccacgtcgt cgtattccag cagcgattta
     1141 cggatatcga agttgcggcc ttccactttc ttctgcgcgt tttcgatgga gcgggtcacc
     1201 cagcggtgct cgatggcttc gccccgctcc atgcccaggg tctgcatcag ccccttcacc
     1261 cggtcggacg caaaaatccg catcaggtta tcttccagcg acagatagaa acgggacgac
     1321 cccggatcac cctggcgacc ggagcggcca cgcagctggt tgtcgatgcg gcgggattcg
     1381 tgacgctcgg tgccgatgat atgcaggccg ccggccgcca gcacctgggc gtgacgttct
     1441 tcccacgctg ccttgatcgc ggctattttt tccggcgtcg ccggatccag cgcctgcacc
     1501 tccacctgcc agttaccacc cagcaggatg tcagttccgc gaccggccat gttggtcgcg
     1561 atggtcaccg cgccaggctg gcccgcctga gcaatgattt ccgcttcctg ggcgtgaaac
     1621 ttcgcgttca gcaccttgtg cgcgatcttt tgtttcttca acaggttcga caggtattcc
     1681 gacgtttcga tggacgccgt gcccaccagc gccggccgtc cagcctcgat gcaacccttg
     1741 atatcctcga tgatggcttc gtacttttcc tccgccgaca tatagatcag gtcgttgtgg
     1801 tcctgccgga tcatcggtcg gttggtggga atgaccacta catccagacc gtaaatctgg
     1861 tggaattcga aggcttcagt atcggcggtg ccggtcatgc ccgacagctt gcggtaaaga
     1921 cggaaataat tctggaaggt ggtggacgcc agtgtctggt tttccgactg gatctttacg
     1981 ccttctttcg cctcgatggc ctgatgaatg ccatccgacc agcgccggcc cggcatggtg
     2041 cgacccgtat gttcatcgac aatgacgatc tggccgttct gcacgatgta ctccacgtca
     2101 cggtgaaaca gcacgtgggc cttcagcgcg gcatgaacat ggtgcagcag gctgaggttg
     2161 ttggcggcat aaaggctgtc gccttcgccc aacagtttgt tctctgtcag cagctcttcg
     2221 atatagacgt gaccttcctc gctcaattcc acctgacggt tcttctcgtc cacggtgaaa
     2281 tggccggtgg tctcctcgcc ttccaccgtg ccacgcttga gtctgggaat cagcaggttg
     2341 atgcgacggt acagctcgga actgtcttca ctggggccgg atatgatcag cggtgtgcgc
     2401 gcttcgtcga tcaggatcga gtccacttcg tccacgatcg cataggccag cggaccctgc
     2461 acccgatcct gcagggtaaa ggccatgttg tcgcgcagat aatcgaagcc gaattcgttg
     2521 ttggtaccgt aggtgatatc ggaagcatag gcagcgcgct tcagatccgg tggctgctgc
     2581 gacaggatca cgcccaccgt caggcccaga aattcgtaga ggggccgcat ccagttggcg
     2641 tcgcgctggg ccaggtaatc gttcaccgtc accacatgca caccctgacc cggcagcgca
     2701 ttcagatagg ccggcagggt tgcggtgagg gttttgccct cgccggtgcg catttccgca
     2761 atacggccct cgtgcagggt tatgccacca atcatctggg catcgaaatg acgcatgccc
     2821 atgacccggc ggctggcttc acgcaccacc gcaaaagcct ccggcaacag gtcattcagc
     2881 tgctcgcctt tctcaagccg ctggcgaaat tcagtggttt ttcctttcag tgcctcgtcg
     2941 gacaacgtct gcaggctggt ctcaagctcg ttgatgcgca ccaccagctt gcccatccgc
     3001 ttgagttcgc gctcgttctt gctaccgaaa atcttgcgta acagtggtcc aaacataagc
     3061 aatcacttct gttcaaaggc tggatttccc tgcgggtaaa ggaaagggct agatacccga
     3121 taaggcaggg aacggctgtg aaggtggcaa ttctaacgtt attttccctg aaggataaag
     3181 caattacgct ttactttagt gagggggcgc acgcttgcgg tgcgcctggg tatcgggacg
     3241 attcaaccct gtttgagatt gaggtagtga gcaggattca ccaccttgcc attgagacgg
     3301 acttcaaagt gtacatgcgg gcccgtggaa cggccggaac tgccgatttt tcccagtgtc
     3361 tgccctttct tgacgatgtc gcccaccttg acgtcgagat cccgggcatg ggcatagcgg
     3421 gtcacgtaac ctccgccgtg gttgacttca acgaattttc cgtagccctt gcgactgccg
     3481 gcaaaggtaa ccaccccggc cgcaaccgac ctgaagtcag cgcccgcctt gcccgccaga
     3541 tccacgcctt catgcattgc caaacggccg gtaaagggat cactgcggcg cccaaatggt
     3601 gacgacagcc agccatgctc cagcggatgc cccgtcaggt ctgcctgctc ctgcaacttg
     3661 cggttggcca gaagggattc cagtacctcc agctgctgct cacgtttgcc gatatagtct
     3721 gccaaccgat ccagttccgc cagaaaaccg gggcgctcat aggctgcatc cagcgcatgc
     3781 tcgggaccgc cgaccggtgg cggacgggaa aaatcgaact cacccccatc cagccgtgcc
     3841 atacccacca ggcgctcgcc cagggcatcc agacgcagca ggcgcgcctg cagctctgcc
     3901 atccgcacgg tcatggcggt gagctgctcc tcggcattaa gccgggcccg acccacttct
     3961 tctgcctggg cagccgcatc gcgctgcaga agtgcggctg cagtcaactc aatgccgcga
     4021 tcgctccacc atatgcctgc catcgcgccc gtcaaaccgg tcgccagcaa cagcgtcagc
     4081 gcaatgccgg ccataaaacc tgcgcgcaga cgcaatgtac gggaatgtcc tgttctggaa
     4141 tcaaatagga taatattcat caggatcact cttgagccaa gggcagaaac aggtttgcat
     4201 cattcgccaa cccttaccgc aggctggata aaacgatgtc agaacccaac atcaaaaatg
     4261 tgcaggaact cctgcggcac aaccggggag taaaaaacct caccgacaag gccggaaaaa
     4321 tacaggaact gcaacgtgca cttactcttg tcctgccgac aatgctggcc gattactgtc
     4381 aggccaggcg cttcgacggc ggcgttcttt atctggatgc tgccacagga tcggtagcaa
     4441 cccagcttcg attcatccag tcgcaactgg ttcaaaaact gcagaagata gctgttttca
     4501 gcacactgga ccgcatcagc atccgggtac aaggcccggc tcaggcggtg cgcaaacctg
     4561 gcaagcgaaa gacaccaccc gtttcggccg aaaactgtca acttctggaa gaggctgcca
     4621 acaacatcag cgataccgcg ctggcggaat ctctgcgcac tctggcgaag accttggaag
     4681 gactatgaca aattctggag ggatttctac aaaatccggc tgaacggccg gactttgtat
     4741 gaggactgcg aactattccg cagcgaatat gggctgtacg taggaaatcg gggaaacctt
     4801 ctgatcgccg aaagtgacga tttcgaatgc atcctgctgg gacaacaatt cccgcaccag
     4861 cttgttgttc aaggcgtgac cggatttgta accgcagaac tccccaatca gactcttgcc
     4921 catcaggtac aggtcaccaa tcgcatccag caccttgtgc cgcacgaatt cgtcttcgta
     4981 gcgcagaccg tcttcattca gaatccggta atcatccacc acgatggcgt tctcggtgct
     5041 gccacccagc gccaggttgt tcttgcgcag cttctcgatg tcgcgcatga aaccgaaggt
     5101 gcgggcacgg ctgacttccc gcacgaatga tgtgctggag aagtcgatgg aaatggtctg
     5161 gttgccacct ttgatgacag ggtggtcgaa atcgatggta aagccgacct tgaatccatt
     5221 gaacggcagg aaggtggcag acttgtcgcc atcggtcacc tgtaccttgc gcttgatgcg
     5281 gatgaactgc ttcagggcgt tctgctcttc gattccagcc gattgcaaca ggaatacgaa
     5341 cggcccggcg ctgccatcca tgatgggcac ttcaggcgcg ctcagatcca catacgcgtt
     5401 gtcgatacca agaccggcaa acgccgccat caggtgctct acagtggaaa cccgcacatc
     5461 gcccttcacc agattggtgg acaggacggt gttggccaca ctgaaagcct tggcttcaat
     5521 ggtgactaca ggatcgagat ccgttcggca gaagactatg cccgtgttgg caggggccgg
     5581 acgcagggtc aggtaaacct tgtcacccga gtgcaggcct ataccggtgg cacggatcac
     5641 gtttttcagc gtgcgctgtc tgatcatgtc tctcacacaa ttaggttaat tgggctaggt
     5701 cagaccctag ctgcccgaat actagcagct tttattaggt tgaccaacct aaccctgttg
     5761 aaattgcgca atttttgaac aatttcaatc tgcctggcgc cgtaaaaatg tcggaatgtc
     5821 gagatattca cggtttacgt cggtttccag cactgcgcga cggcgcactt cctgctgctg
     5881 acggcgggcc cgatctactg ccggctgctc gaaacggcga tagtcggtta caggttctgg
     5941 agcagaccga cgggtcaccg actcaaccgc gcggacgggt gctgcctgct cgctgacagc
     6001 gcccatgccc aggccggtgg ctaccacggt tacccgcagt tcgtcgccca ggcttggatc
     6061 gataacggta ccgacgacga cagtggcgtt gtcggaggcg aactcttcca cggtgtcacc
     6121 cacttcagag aactcaccca gggacaggct ttcgctggca gtgatgttca ccaggatgcc
     6181 gcgggcgccc tgcaggttga cgtcttccag cagcgggctg cggatggcgg cttcagcggc
     6241 ttcgcgggca cggttttcac ccgtggcgcg gccggtaccc atcatggcca tgcccatttc
     6301 ggacatcacg gtgcgcacgt cggcaaagtc gacgttgatc atcccgggac ggatgatcag
     6361 gtcggcgatg ccttgtacgg cacccagcaa cacgtcgttg gcggccttga aggcatccag
     6421 caggctggtt ttgggaccga gcacatccag cagcttttca ttggggatgg tgatcaggga
     6481 gtcgacgttc tcgctcaggt gcttcatgcc ttccagggcc acctgcatac gtttcttgcc
     6541 ttcgaagggg aaaggcttgg tcaccacggc gacggtcagg atgcccattt cctttgcgat
     6601 ttcggccacg atcggcgcgc caccggtgcc ggtgccgccg cccatgccgg cggtgatgaa
     6661 caccatgtcg gcgccgttga gaacttcgcg gatgcgttcg cggtcttcca gcgcggcttc
     6721 acggcccacg ttgggattgg cacctgcgcc caagcccttc gtcaccgcgc cacccagctg
     6781 aagcagggtt ttggcattca tgttcttgag tgcctgtgca tccgtgttgg cacaaatgaa
     6841 ttcaacgccg tcaacctggc tggatagcat gtgctcaacg gcattgccac caccaccacc
     6901 tacgccaacg actttgataa ctgcgttctg cggtacgtta tcaacgagtt cgaacatatt
     6961 taatctcccc ttccttccgc tatggttctc tttgacaaga gactgttaaa agttgccttt
     7021 gatccaggtc ttcatgcggt ccatgaaacc cgcttgtggc acgggctgtc ccttctgctg
     7081 ggcagcaccg gcttctattt gtttttgtcc gtacagcaac aggccgacac cggtggagta
     7141 gatcggattc ttcaccattt cagtgagacc cgaaattccc tgcggcgttg ccagtcgcac
     7201 cggcatgtgg aaaatttctt ctgccagatc gaccgcgcct tccatgcggg ctgagcctcc
     7261 ggtgagcacg atgccggcgg cgatcatgtc ttcatagccg gagcggcgca gttcggcctg
     7321 caccagcgaa aacagttcgt cataacgggg ctccaccact tctgccagag cctggcgcga
     7381 cagttcacgc ggcgggcggt caccgacact ggccacctta atcgattcat caggccccgc
     7441 caactgggtt aatgcacagg cgtacttgat tttgatgtca ttggcatttt cagtgggtgt
     7501 gcgcagagcc atggcaatgt cgttggtgac ctggtcgcca gcgatgggaa tcaccgcggt
     7561 atgacggatg gagccttcgg tgaagacggc aatgtctgtg gtgccaccgc cgatgtccac
     7621 catgcagacg cccagttcct tttcatcatc agtgaggacg gcataggcgg aagcaagctg
     7681 ctccaggatg atgtcttcca cttccagacc gcagcggcgc acacagcgct cgatgttctg
     7741 cgaggcgttc accgcgcagg tcaccagatg caccttggct tccaggcgca cgccggacat
     7801 gccgagcggc tccttgatgc cttcctgcga atcgatgatg tattcctgcg gcaggatgtg
     7861 gaggactttc tgatccgctg gaatgggtac ggcctgggcg gcgtcgatga cccgttccag
     7921 atcggcgcgg ttcacttcgc ggtcacggat cgcaacgatg ccgtggctgt tcaggctgcg
     7981 gatgtggctg ccggcgatgc cgacatagac cgagtggatc tggcagccgg ccatgagttc
     8041 ggcttcctcg atcgcgcgct ggatggactg cacggtggat tcgatgtc
//
LOCUS       DASUEG010000415         6419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1442729, whole genome shotgun sequence.
ACCESSION   DASUEG010000415 DASUEG010000000
VERSION     DASUEG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442729"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="ggt"
                     /locus_tag="VFX11_07745"
     CDS             <1..1623
                     /gene="ggt"
                     /locus_tag="VFX11_07745"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4938551.1"
                     /translation="APRDFAVASAHPLATQAGIKILEQGGNAFDAAVTVAAVLGVVEP
                     YSAGIGGGGFWLLHRAKDGKTLMLDAREMAPGAASADMFLDGKGGVDREKATNSPLSA
                     GIPGQAAALDHLARHYGKLPLKTTLAPAIAFAESGFPSNPVYAALAGFRAPVLMMNPD
                     SARIFLADGQPLNTATIRQPELATTLKALADKGFDGFYKGEVAQKLVAGVKAGGGIWT
                     LEDLAGYTLVEREPVHFKYRDAEVWSAPPPSSGGVALGQMFGMLQQFDLTHRNEADRT
                     HLLIEVMRRAYRDRAEFLGDPDFVTVPVQKLLDPVYLEEIAANISGSHATPSKDLAPV
                     PPPPSGTHTTHFSIIDKAGNRVSATLSINLPFGSGYTVAGTGVLLNNELDDFSAQPGS
                     PNAFGLVGSAANAIAPRKRPLSSMTPTFMEYGPADNRQFAIIGTPGGSRIISMVFLGL
                     LEALDGKGPQAWVDRPRFHHQYLPDMVEYEPGAFTPVLGAALDAKGHRLQETERVYGD
                     MHVIRWTLKNGMVEAASDKRRLGSARTGAAAH"
     gene            complement(1701..2033)
                     /locus_tag="VFX11_07750"
     CDS             complement(1701..2033)
                     /locus_tag="VFX11_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938552.1"
                     /translation="MRSLKQNLKSATLALAMAAFAGAPALSNAEIVTERPTGLEMTAN
                     AVLVRPLTLVATVVGTVVYVVSLPFSALGGNAGEAGQTLVVTPFKATFVRCLGCTEKH
                     LAYAERQE"
     gene            complement(2202..3014)
                     /gene="mutM"
                     /locus_tag="VFX11_07755"
     CDS             complement(2202..3014)
                     /gene="mutM"
                     /locus_tag="VFX11_07755"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX4938553.1"
                     /translation="MPELPEVETTRRGIAPHIIGKRVKRIEVRQPSLRWPVPEEIHAA
                     EGRKLQAVERRGKYLLLRFANGTIITHLGMSGSLRIALPDEPPLFHDHVDLLCSDGTR
                     LRYCDPRRFGAWLWTEDDPLQHPLLAELGPEPLTDAFTADYLAQRAAGRKTAVKAFLM
                     DSHIVVGVGNIYANEALFMAGIHPKRAAGKVSLVRYEKLVQAVKQVLADSIEMGGTTL
                     RDFVGGDGKPGYFQQTLRVYGRGGQPCRRCKAELHEIRLGQRATVFCKTCQT"
     gene            3098..5080
                     /locus_tag="VFX11_07760"
     CDS             3098..5080
                     /locus_tag="VFX11_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4938554.1"
                     /translation="MNATKMLRLGVDTGGTFTDFVLLDAAGLRIHKVLSTPEAPEQAI
                     LQGIAELGLQDAVREGGVRIVHGSTVATNAALEGKGVRTAFVTNRGFADLLTLGRQTR
                     RELYNLQPPLPQPPVPAQLSFETGGRVDAQGQTLDPLTEADIAHLLEQLREANVEAVA
                     VCLLFSFLDPAAEQRLLAALSPHWHTSISSAVLPESGEYERGIATWLNASLGPRVKQY
                     LARLRDGVAPSPLAIMQSTGGTLDTDQAGERAVNLLLSGPAGGMAAARFIAGQLDSSR
                     LLTFDMGGTSTDVALIDGDIRLTNEGRIGPWPVAVPQVDMHTIGAGGGSIAWIDAGGL
                     LQVGPQSAGAAPGPACYGRGGTLPTVTDANALLGRLRPDYFLGGRMTLDLDAARRALQ
                     PIADRLGLSLADTAQGILSIANEHMARALRVISVQRGHDVRQFRLCSFGGAGGLHVCA
                     LADALGMDKALVPVHGGVLSALGMVVAPAERQASRALGGLLQEQAARSHAALDTLKQE
                     AITALGQGTGPAITLRPSLDLRYRGQRYFLNIPWQGDSQQAEDDFHAAHAARFGHQLP
                     MAVEVVNARVQAQIAGEPVALPEWPAGPDAAPVQRCQLEGSGEVLLYRREDLAQGQVV
                     NGPALVVEAVSTLLIETGWQATPDRHGHLLLDRIGA"
     gene            complement(5111..6343)
                     /locus_tag="VFX11_07765"
     CDS             complement(5111..6343)
                     /locus_tag="VFX11_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4938555.1"
                     /translation="MEIFLARQPVFDRQLNAIAYELLYRGGRVQHAVIDDPGKASMQV
                     LVNAFSEIGLEKITNGKPALVNIPHELLVTGALPKGLQKLLIPEVLEDVAVDKNVIQE
                     VKNLVSLGYRVALDDFVYSDAWKPLIAVASFIKLDVMALGVERVKEQLARIRLCGNTK
                     GKLLAEKVETHEEFELYKALGFDYFQGYFLCRPHVMAANSVPASHMVITSLLGELGRD
                     DYDVDSVARIICQDPRLSYKLMRVVNSASFGLSRTVKTVEDTIVILGSYELRRWASML
                     ALSTVDNKPHELLITAIVRAKMCELVAKQLKRQNPGSYFTAGLFSLLAALLDRPLEDI
                     VSQMPLSAELELALVTGGGDIGQVLRAVEAYEAGRFEESLLQGMTAALFWDIYLEALE
                     WSAVASHAMLSRGENGKG"
BASE COUNT         1160 a         2143 c         2011 g         1105 t
ORIGIN      
        1 gcacccaggg attttgcggt ggccagcgcc catccgctgg ccacccaggc cggcatcaag
       61 atcctggaac agggtggcaa cgcgtttgat gccgcggtga cggtggccgc cgtgctgggc
      121 gtggtggagc cctacagcgc cggtattggc ggcggcggtt tctggctgct gcaccgggcc
      181 aaagatggca aaaccctgat gctggacgcg cgggaaatgg cgcccggcgc cgccagcgcg
      241 gacatgtttc tggacggcaa gggcggcgtc gaccgcgaga aagccaccaa ctcaccgctg
      301 agcgccggca tccccggcca ggccgccgcg ctggatcacc tggcccgcca ctatggcaag
      361 ctgccgctga aaaccaccct ggcgcccgcc attgccttcg ccgaaagcgg ctttccctcg
      421 aatcccgtct atgcggccct ggccggcttt cgcgctccgg tactgatgat gaatcccgac
      481 agcgcccgca tcttcctggc agacggccag cccctcaaca ccgccaccat ccgccagccg
      541 gaactggcga cgacgctgaa ggcactggcc gacaaaggct tcgacggttt ttacaaaggc
      601 gaggtggcgc agaaactggt ggccggcgtg aaggccggcg gcggcatctg gacactggag
      661 gatctggcgg gctacacgct ggtggaacgc gagccggttc atttcaaata ccgcgacgcc
      721 gaggtctggt ccgcgccgcc gccgtcgtcg ggcggtgtcg ctctggggca gatgttcggc
      781 atgctgcaac agttcgatct cacccaccgg aacgaggccg accgcactca cctgctgatt
      841 gaagtgatgc gccgcgccta ccgcgaccgc gccgaatttc tgggtgatcc ggatttcgta
      901 acagtgccgg tgcagaagct gctggacccg gtgtacctgg aagaaatcgc cgccaacatc
      961 tccggcagcc acgccacgcc gagcaaggat ctggcgccgg tgccaccccc gccgtccggc
     1021 acccacacca cgcatttttc catcatcgac aaggccggca accgcgtgtc cgccaccctg
     1081 tccatcaacc tgccgttcgg ctccggctac acggtggcgg gcaccggcgt gctgctcaac
     1141 aacgagttgg acgacttctc cgcccagcca ggctcgccca atgccttcgg tctggtgggc
     1201 agcgccgcca acgccatcgc gccgcgcaaa cgccccctgt cgagcatgac acccaccttc
     1261 atggaatacg gcccggcgga caaccgccag ttcgccatca ttggcacccc cggcggcagc
     1321 cgcatcatca gcatggtgtt cctcggcctg ctggaagcac tcgacggcaa aggcccgcaa
     1381 gcctgggtgg accgcccccg cttccaccac cagtacctgc ctgacatggt ggagtatgag
     1441 cccggcgcct tcacgccagt cctgggtgcc gcgctggacg caaagggcca ccgactgcag
     1501 gaaaccgagc gtgtctatgg cgacatgcac gtgattcgct ggacgttaaa gaatgggatg
     1561 gtggaagcgg catccgacaa gcgccggctg ggctcggccc ggaccggggc tgcggcgcat
     1621 tgaattgtga attgaatgca gacgccgccc ggatgccggg cggcgggaga agacgatcga
     1681 acccggcgac aggcgccgga ttattcctgc cgttcggcgt aggccaggtg tttttcggta
     1741 cagcccagac agcgcacgaa cgtggccttg aacggtgtta ccaccagggt ctggcccgct
     1801 tcaccggcgt tgccacccag ggcagaaaac ggcagcgaca ctacgtacac cacggtaccc
     1861 accacagtgg ccaccagcgt cagcggacgc accagcaccg cattggcggt catttccagt
     1921 ccggtggggc gctccgtcac gatctcggcg ttgctcagcg cgggcgcgcc ggcgaacgcg
     1981 gccatggcca gtgccagggt ggcggatttg agattctgtt tcaggctacg cataacgatt
     2041 cctcttcttg ttcttgcgaa ggatgccacc ggaatggatt gatttgagtg tattcgctaa
     2101 tagtgcggcc ttccactttg taaggcaatg tcatgacctg acccgccgat ttttccgact
     2161 ggatcgcatt ttggctgcat tgtgagcgcc gcacagatct ttcaagtctg gcaggttttg
     2221 cagaaaacgg tagcccgctg gcctaggcgg atttcatgga gctccgcctt gcagcggcgg
     2281 cacggctggc caccacggcc atacacccgc aacgtctgct ggaaataacc cggcttgccg
     2341 tcgccgccta cgaaatcccg caacgtggta ccgcccatct cgatggaatc cgccagtacc
     2401 tgcttcaccg cctgtaccag cttttcgtag cgcaccaggc tgaccttgcc cgccgcccgt
     2461 ttggggtgga tgcccgccat gaacagggcc tcgttggcgt agatattccc cacccccacc
     2521 acaatatggc tgtccatcag aaaagctttc accgccgttt tgcggccagc agcacgctgg
     2581 gcaagatagt cggcggtgaa ggcatccgtc agcggctcgg ggcccagctc cgccagcagc
     2641 gggtgttgca gcggatcgtc ctcagtccac agccaggcgc cgaagcggcg cggatcgcag
     2701 tagcgcaggc gggtgccgtc actgcacagc aggtcgacgt gatcatgaaa cagcggcggc
     2761 tcgtccggca gcgcaatgcg cagactgccc gacatgccca gatgggtgat gatggtgccg
     2821 ttggcaaaac gcagcagcag gtattttcca cgccgctcca ccgcctgcag cttgcggccc
     2881 tcggcggcgt gaatctcctc cggcaccggc cagcgcaggc tgggctggcg cacctcgatc
     2941 cgcttcaccc gcttgccaat gatatgcggc gcgatgccac ggcgggtggt ttccacttca
     3001 ggtaattcgg gcataggttt cggctgcaat agtcggtttc gactgcggta gaatgcgaag
     3061 cgctccatta ttcagaaacg gcggggaagt tgaaatcgtg aatgcgacaa aaatgctgcg
     3121 cctgggcgtg gacaccggcg gcacctttac cgatttcgtg ctgctggatg cggcgggcct
     3181 gcgcattcac aaggtgctgt ccacccccga ggcgccggag caggcgatcc tgcagggcat
     3241 cgcagaactg ggcctgcagg acgccgtgcg cgaaggaggt gtgcgcatcg tgcatggcag
     3301 cacggtggcc accaacgcgg cgctggaagg caagggcgtg cgcacggcat tcgtcaccaa
     3361 tcgcggtttc gcggatctgt tgacccttgg ccggcagacc cggcgtgaac tctacaacct
     3421 gcagccgccg ctgccgcagc cgcccgtgcc ggcgcaactc agttttgaaa ccggcggccg
     3481 ggtggatgca cagggacaaa ccctggaccc tctgacggaa gcggacattg cacacctgct
     3541 ggaacagctg cgcgaggcca acgtcgaagc cgtcgccgtc tgcctgctgt tttcctttct
     3601 cgatccggct gccgagcagc gtttgctcgc tgcactgtca ccccattggc acaccagtat
     3661 ttccagcgcg gtactgccgg aatccggtga gtacgagcgc ggcatcgcca cctggttgaa
     3721 cgcttcgctg gggccgaggg tgaaacagta tctggcaagg ctgcgggacg gcgtggcacc
     3781 gagccccctg gccatcatgc aatccaccgg cggcaccctg gacacagacc aggccggcga
     3841 gcgggcggtg aacctgttgc tgtccggccc tgcgggtggc atggcggcgg cgcgtttcat
     3901 tgccgggcag ctggattcgt cccgcctgct caccttcgac atgggtggca cctccaccga
     3961 cgtcgccctg atcgacggcg acatccgcct caccaatgag ggccgcatcg ggccctggcc
     4021 tgtggctgtg ccgcaggtgg acatgcacac catcggcgcc ggcggcggct ccatcgcctg
     4081 gatcgacgcc ggcggtctgc tgcaggtcgg cccgcagtcc gctggcgccg cacccggacc
     4141 ggcctgctat ggtcgcggcg gcacccttcc gaccgtcacc gacgccaacg ccctgctggg
     4201 gcggctgcgg ccagactact tcctgggcgg acgcatgacc ctggacctgg acgccgcccg
     4261 ccgcgccctg caacctatcg cggaccggct cggactgtcg ctggccgata ccgcccaggg
     4321 catcctgagt atcgccaacg aacacatggc ccgggctctg cgggtgatct cggtgcaacg
     4381 gggacatgac gtgcgccagt tccgtctgtg cagcttcggt ggtgctggcg gcctgcacgt
     4441 ctgcgcactg gccgatgcgc tgggcatgga caaggcactg gtacccgtcc atggtggcgt
     4501 gctgtcggca ctcggcatgg tggtggcacc ggccgaacgc caggcgagcc gggcgctggg
     4561 tggtctgctg caggaacagg ctgcccgcag ccatgcagca ctggacacgc tgaagcagga
     4621 agccatcacc gccctcgggc aaggcactgg gcctgccatc accctcagac ccagcctgga
     4681 cctgcgctac cgtggccagc gctacttcct gaacattccc tggcaaggtg attcccagca
     4741 ggccgaggac gacttccacg ccgcccatgc cgcccgtttc ggccaccagc tgcccatggc
     4801 ggtggaagtg gtgaatgcgc gggtgcaggc acagattgcc ggggaaccgg ttgccctgcc
     4861 ggaatggccg gcggggccgg atgcggcgcc cgtacaaagg tgtcagctgg aagggtcggg
     4921 tgaagtgctt ctttaccggc gcgaagatct ggcacagggg caggtggtga acgggccggc
     4981 gctggtggtg gaagcggttt caaccctgct gatcgaaaca ggttggcagg caacgccgga
     5041 caggcacggc catctgctgc tcgaccggat cggcgcgtaa aaataacatc ggaagcccga
     5101 tcaacggcgc tcagcccttg ccgttctcac cccgggacaa catcgcgtgg ctggccacgg
     5161 cagaccattc cagcgcttcc aggtaaatgt cccagaacag cgctgcggtc attccctgca
     5221 gcagggattc ctcgaatcga ccggcttcat acgcctccac cgcccgcagc acctggccga
     5281 tgtcgccgcc gccggtgacc agcgccagct ccagctcagc cgacagcggc atctgcgaca
     5341 ctatgtcctc cagcggccgg tccagcagcg cggccagcag ggaaaacagg cccgccgtga
     5401 aataactgcc cgggttctgc cgcttcagct gcttggccac cagttcgcac atcttggccc
     5461 tgacgatggc cgttatcagc agttcgtggg gtttgttgtc gaccgtggac agcgccagca
     5521 tgctggccca gcgccgcaac tcgtaggacc ccaggatgac gatggtgtcc tccaccgtct
     5581 tcaccgtgcg cgacaacccg aacgacgccg aattcaccac ccgcatcagc ttgtaggaca
     5641 ggcgcgggtc ctggcagatg atgcgcgcca cactgtccac gtcatagtcg tcccggccca
     5701 gttcccccag caggctggtg atcaccatgt gggacgccgg caccgaattg gccgccatca
     5761 cgtgggggcg gcacaggaaa tagccctgga aatagtcaaa gcccagggcc ttgtacagtt
     5821 cgaattcctc gtgggtttcc accttctccg ccagcagttt gcccttggtg ttgccgcaca
     5881 ggcggatgcg tgccagctgc tccttcaccc gctccacccc cagcgccatc acgtccagct
     5941 tgataaagct ggccacggcg atcagaggct tccaggcgtc ggaatagacg aaatcatcca
     6001 gcgccacccg gtaacccagg gacaccagat tcttcacttc ctggatcacg ttcttgtcca
     6061 ccgccacatc ttccagcacc tcgggaatca gcagcttctg tagacccttg ggcagggcgc
     6121 cggtgaccag cagttcgtgg ggaatgttga ccagggcggg cttgccgttg gtaatttttt
     6181 ccaggccgat ctcggagaag gcattgacca gtacctgcat cgaagccttg ccgggatcgt
     6241 cgatcaccgc atgctgcacg cggccgcccc ggtacagcaa ctcataggca atggcattca
     6301 gctggcggtc aaaaaccggc tgccgcgcta gaaatatttc catgaagatt gagccccgga
     6361 gggtgacaca actggcatgg ttaaagtgtg gcagaaaaac ccggaaccac aacgcggac
//
LOCUS       DASUEG010000416         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_112024, whole genome shotgun sequence.
ACCESSION   DASUEG010000416 DASUEG010000000
VERSION     DASUEG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112024"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1893
                     /locus_tag="VFX11_07770"
     CDS             259..1893
                     /locus_tag="VFX11_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938556.1"
                     /translation="MGDDYMHYAVLNAKNLPIGKPDDLSLFGLFSFINGDPERNRQAM
                     DLGVIPWWTYGEMKYAFWRPISEVFHWIDHQLWPRSPALMHLHSILWYLAICVLLARL
                     YRRTLGAGSVTLLALALYALDSTHGFTVSWIANRNALISVLFGLLAIWFHLRWREEDK
                     IGLAALSLLCMLASLLSAELGISAFGYLGAYALTLDKRGRVKGCLATVPHFLLIVAWW
                     IFYKQAGFGAVNADAYYIDPAAQPLGFLAVVGERLPVLLASQWGLIPAEIYGFVLEHD
                     LRFVAGCAIFLLLVGVPLVLSCRRQPAVQFWLLGMLFSVLPAATALPHDRVLLFAGAG
                     ACAALAHLLLSVLPKGASIQGLPGFYTKPIAYLLVLMHLILSPLLLPLMAYSTKLWAG
                     LIPQQPSYFPSQEPVDQQRLVLFGAPLPSALAIAPFRFYRQESIPERIWTISTLDANF
                     QFTPIADNALQIDLPTGFIQLGEIKVRDLQRFPFHEGETIELSGMTITVSNLNTEGKP
                     TRLVLTFDRALTDSHLKFLRWDGKKNDYATLNFQSP"
     gene            complement(2335..2449)
                     /gene="rrf"
                     /locus_tag="VFX11_07775"
     rRNA            complement(2335..2449)
                     /gene="rrf"
                     /locus_tag="VFX11_07775"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2578..>2910)
                     /locus_tag="VFX11_07780"
     rRNA            complement(2578..>2910)
                     /locus_tag="VFX11_07780"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          615 a          850 c          749 g          696 t
ORIGIN      
        1 ataaacgaca aaaatatacc gtgtatacac ccggccatca tcaaacagac cggatattca
       61 cgacccaaca cccagaatcg gaccaacgtc cttgcagatg cagttgcgca aactttacac
      121 ttcccaaatc tatataagta gacacaataa aaaataatga ctacaaaact tttttccagc
      181 cgtgatttca accgccgctc aatcctcgtc ctcacgcttg cttttttact ttgcttaccc
      241 gcactctggg ccggcttcat gggcgacgat tacatgcatt acgccgtcct caatgccaaa
      301 aacctgccca ttggcaaacc ggatgacctg tccctgtttg gcctgttttc cttcattaat
      361 ggcgatcccg agagaaaccg gcaggccatg gacctcggcg tcatcccctg gtggacctat
      421 ggcgaaatga agtacgcctt ctggcgtccc atcagcgagg tattccactg gattgatcac
      481 cagctctggc cgcgcagccc tgccctgatg caccttcaca gcatcctgtg gtatctcgcg
      541 atttgcgtgt tgctggccag attgtatcgc cgcacgctgg gggccggatc ggtcaccctg
      601 ctggcgttgg cactgtatgc gctggacagc acccatggat tcaccgtcag ctggatagca
      661 aaccgtaacg cgctcatcag cgtcctgttc gggctgctgg ccatctggtt ccacctgcgc
      721 tggcgcgagg aagataaaat cggactggct gccctttccc tcctttgcat gctggccagt
      781 ctgctttctg cagaactcgg aatttccgcc ttcggctatc tgggggccta cgcactgaca
      841 ctggacaagc gcgggcgagt gaaaggctgc cttgccacgg tgccccactt tctgctgatc
      901 gtcgcctggt ggatttttta caagcaggcc ggtttcggcg cagtcaatgc ggatgcctac
      961 tatatcgatc cggcggcaca accactgggt ttcctggctg tggtgggcga gcggttgccg
     1021 gttctgctgg ccagccaatg gggtcttatc ccggcagaga tttacggctt cgtactggaa
     1081 cacgatctcc gattcgtcgc gggttgcgcg atcttcctgc tgctggtggg cgttccactc
     1141 gtgctgagtt gccgacgcca gcctgccgtc cagttctggc tgctgggtat gctgttttca
     1201 gttctgcccg ctgccacagc cctgccccat gaccgcgtgc tgctgtttgc cggtgctggc
     1261 gcctgtgctg ctctggccca tctgctactg agcgtcctgc ccaaaggagc atccattcag
     1321 gggctgccgg gtttctatac caagccaatc gcctacctgc tggtacttat gcatttgatc
     1381 ctgagcccac tgctgctgcc gctgatggcg tacagcacaa aactctgggc aggcctgata
     1441 ccacagcaac cgtcctattt cccgagccag gaacctgttg atcaacaacg gctggtgctc
     1501 ttcggtgcgc ccctgccatc cgctctggcc attgcaccgt tccgctttta ccggcaggaa
     1561 tccattccag agcgcatctg gacgatctcc acgctcgatg ccaatttcca gttcacaccg
     1621 atcgccgaca atgcattgca gattgatctg cccaccgggt tcatccaact cggcgaaatc
     1681 aaagtccgcg acctgcaacg cttccctttc cacgaagggg aaaccattga actctccggc
     1741 atgaccatca ctgtctcgaa cctgaatact gaaggcaagc caacccggct ggtcctgaca
     1801 tttgatcgag cgctgacaga cagccatctg aaatttctgc gctgggacgg caagaagaac
     1861 gactatgcaa cgctgaattt ccagtcacct taatcattcg ctctttccgc cggctggcgg
     1921 gtcggcgaac aggcaagtta tttactcaga agtgctgggt cagcagtgga cgcggctcag
     1981 cacttctgcc ggactttagt taataaatat tggaaccgag ttcaatcctc catgctccgg
     2041 tcagtggatt acaaatcaca tctcgatcca tccggattgc gtgcgcctcg ctccgcaacg
     2101 tgattgtcag aactcaggta aatgcagcag tccgaccgat gctgctattg ttcgggagtg
     2161 ctgttcttaa acgcgaagca tgcagggagt gaaggcagtc gggttgggtg gaggatccag
     2221 aggttgcggt gtcagggatt ggcgggaagg ggggaggaag gcatgggagc gattgaagct
     2281 gcgccgcaaa gtaaaaagcc ccgctcgtta gtcgagcggg gctttttgat aggagcctga
     2341 cgatgaccta ctctcacatt gggaatccaa cactaccatc ggcgatgctg cgtttcactt
     2401 ctgagttcgg aaagggatca ggtggttcca cagctctatt gtcgtcaggc aaactggcat
     2461 gatgccgcgt ttgcatattg cagggcgcac atcaaattcg ttaacgaggt aattgttcaa
     2521 gtcgcaagct attggttatg acattcagcg atgccatcta accttgctta gggttatatg
     2581 gtcaagccgc acggacaatt agtattggtt agcttaacac ctcacagtgc ttccacaccc
     2641 aacctatcaa cgtcgtagtc ttcgacggtc ctttaggagg atcaagtcct cagggagatc
     2701 tcatcttgaa gggggcttca cgcttagatg ctttcagcgt ttatcctgtc cgaacatagc
     2761 tacccggcaa tgccactggc gtgacaaccg gaacaccaga ggttcgttca ctccggtcct
     2821 ctcgtactag gagcaactct tctcaaatct ccaacgccca cggcagatag ggaccgaact
     2881 gtctcacgac gttctaaacc cagctcgcgt
//
LOCUS       DASUEG010000417         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1215444, whole genome shotgun sequence.
ACCESSION   DASUEG010000417 DASUEG010000000
VERSION     DASUEG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215444"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFX11_07785"
     CDS             <1..221
                     /locus_tag="VFX11_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4938557.1"
                     /translation="IRSDRCITDIALMLGFCDSSAFSKAFKKWTGESPSEFKQKHALK
                     EFRYFSNGDVTGGAGLLPSDKTGDQHLF"
     gene            463..978
                     /locus_tag="VFX11_07790"
     CDS             463..978
                     /locus_tag="VFX11_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938558.1"
                     /translation="MLTTSIDEHFEVAVEFMDNAQKLHIVDNILERASELAGDITDSV
                     MAEFYRGDTEARNLFTHHCPVDTIRLEAGMVEQALYCYMRWFTSPGEIRILLLGSVPH
                     HVETLKVPVNYYHRFLQAMATVIRKTIPAESREEIDVWNEICGDLGEIVDAANQYVYR
                     AERIGKEAVHG"
     gene            complement(1069..2211)
                     /locus_tag="VFX11_07795"
     CDS             complement(1069..2211)
                     /locus_tag="VFX11_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009158230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX4938559.1"
                     /translation="MSTTPQELTQALESLSAQVSDWDHTDALQTAEGRAIVAAKMAEL
                     DLADSHSIIRFGSAAQSGLQAISQEMLGGVRNKDMGKAGDTLREMVTSLRGFSTTELD
                     PNRPPSWWEKLLGKTKPAAEFVARYEQVRDQIDRITNELLQHETVLLKDIKSLDKLYE
                     RTLAFYRELAFYIRAGEEKLRQLDEETIPNFTRDATAGSNDSLLAAQKLRDLRAVRDD
                     LERRVHDLKLTRQVTMQSLPSIRLVQENDKALVIRINSALVNTVPLWETQLAQALTIG
                     RSQDAANTIRAVNDLTNDLLTQNAENLRQANRTVREQNERGVFDMEAIKQANAHLIAT
                     IEESLQIADEGRKKRVQAEQELKEMEGVLKGALQGTVRQEKPPVQG"
     gene            complement(2221..3129)
                     /locus_tag="VFX11_07800"
     CDS             complement(2221..3129)
                     /locus_tag="VFX11_07800"
                     /inference="COORDINATES: protein motif:HMM:NF021617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEX4938560.1"
                     /translation="MNPPVRDARHPGPGNARPEPTPEELFRKARHFVKTRLMLWERSM
                     FLVPLPLMFMGLYSAITGSAIEMAGRFGGLALLLLSAWLLLEGQKAHHAYDAREVAKP
                     PAFPRKLFASLLTGGAVAVTSLFGFLPLLHEPVTAAVYSVVATLLHSIAFGLDPMKSK
                     GLQGYSRQDANRLLQALETGERLMEETLAAASTFPDRRLQQHVMRLVQEARAVFRAIE
                     KDPRDLQRSRKFMAVYLQGARDATVKLAHIKARNPGLQEYADYERLLTDLEQQFIRQQ
                     QSLLLNDRTELDVEVEVLRERIKQER"
BASE COUNT          625 a          950 c         1004 g          757 t
ORIGIN      
        1 tgatcaggag tgaccgctgc atcacggata tcgccttgat gttgggtttt tgcgattcct
       61 ccgccttcag caaggctttc aagaagtgga ccggcgaatc ccccagcgag ttcaagcaga
      121 aacatgcgct gaaggagttc cgctattttt ccaatgggga tgtgaccggt ggcgcgggct
      181 tgctaccgtc cgacaagacc ggcgatcagc atttgtttta gtcacaacga taacggctgt
      241 cacggtttca gtgtcggcct tcagcgagcc agtcaggttc cacccggcgg ctcccgccat
      301 agccaattct tttctgcaaa tgtacagcgc tgttgatcat gatcaatgcg gcgttatctc
      361 agtccggcga ttcttggttt tttccgcccc ggcttttaaa agccagggga attgctgcat
      421 cccattcatc agcctgcatc tgcaaattgg aaacgatggg cattgctgac gacatccatt
      481 gatgaacatt ttgaagtagc cgttgagttt atggataacg cacaaaaact tcatatcgtc
      541 gacaacattc tggaacgcgc atcggaattg gcaggcgata tcaccgattc agtgatggcc
      601 gagttttacc ggggggatac tgaagccagg aatttattca cgcatcattg cccggtggat
      661 acgatcaggc tcgaagcggg catggtcgag caggcgcttt attgttacat gcgctggttc
      721 acgtcaccgg gtgaaatcag aattctgctt ttggggtcag tgccacatca tgttgaaacg
      781 ctgaaggttc cggtgaatta ttaccacagg ttcctgcagg ccatggcaac cgtcatcaga
      841 aagaccattc ccgctgaaag ccgggaagaa atcgatgtat ggaatgaaat ctgcggcgac
      901 ctgggtgaaa tcgtcgatgc cgccaatcag tacgtctatc gcgcagaacg gatcggcaaa
      961 gaagccgttc acggctgatg gaggttaccg gtctgatgcc ggcagaggat caggccagtc
     1021 atcggtgcaa gactgttttg tattgccttc agttctactg accggcactc aaccctgaac
     1081 cggcggtttt tcctgccgca cagtcccctg cagcgcacct ttcagcacgc cttccatttc
     1141 cttcagttcc tgttccgcct gcacccgctt ctttctgcct tcgtccgcaa tctgcagtga
     1201 ttcttcgatg gtggcaatca ggtgggcgtt cgcctgcttg atcgcttcca tgtcaaacac
     1261 gccgcgttcg ttctgttcgc gcacggtacg gttggcctga cgcaggttct cggcattttg
     1321 cgtaagcaga tcgttggtga gatcgttcac cgcgcggatg gtgttggccg catcctgcga
     1381 gcgtccaatc gtcagcgcct gggccagttg cgtttcccac agtggcacgg tgttcaccag
     1441 cgcggaattg atgcggatca ccagcgcctt gtcgttttcc tgcaccagcc ggatcgaggg
     1501 cagggattgc atcgtcacct gccgcgtcag tttcaggtcg tgcacgcggc gctccaggtc
     1561 atcgcgcacg gcgcgcaggt cccgcagctt ttgcgcggcc agcaggctgt cgttgctgcc
     1621 ggccgtggcg tcgcgggtga aattgggaat ggtttcctca tcaagctggc gcagcttttc
     1681 ttcgccggcg cggatgtaaa acgccagctc gcgatagaat gccagcgtgc gttcgtacag
     1741 tttgtcgaga cttttgatgt ccttcagcag cacggtttcg tgctgcagca attcgttggt
     1801 gatgcggtcg atctggtcgc gcacctgctc atagcgggcg acaaattccg ctgccggttt
     1861 ggtttttccc agcagttttt cccaccagct gggcgggcgg ttgggatcga gttcggtggt
     1921 ggaaaaaccg cgcagcgacg tcaccatttc ccgcagcgtg tcgccagcct tgcccatgtc
     1981 cttgttgcgc acaccgccca gcatttcctg gctgatggcc tgcaggcccg actgcgctgc
     2041 tgaaccaaag cggatgatgg agtgggaatc cgccagatcc agttccgcca ttttcgcggc
     2101 aacgatggcg cggccttcgg cagtctgcag tgcatcggtg tggtcccagt cgctgacctg
     2161 ggcggacagg gattccaggg cctgcgtcag ttcctgcggt gtcgtgctca tgctgtttcc
     2221 tcaacgttcc tgtttgatgc gttcgcgcag aacttccact tccacgtcca gctcggtgcg
     2281 gtcgttcagc aacagacttt gctgctggcg gatgaactgc tgttccagat ccgtcagcag
     2341 ccgttcgtag tcggcgtatt cctgcagccc gggattacgc gccttgatgt gcgccagttt
     2401 gacggtggca tcgcgggcac cctgcaggta caccgccatg aatttgcgtg aacgctgcag
     2461 atcgcgcgga tctttttcga tggcgcgaaa gacggcgcgg gcttcctgca ccagccgcat
     2521 cacatgctgt tgcaggcgac ggtcgggaaa agtgctggcg gcggcgaggg tttcttccat
     2581 cagccgttcg cccgtttcca gcgcctgcag caaacgattg gcgtcctgcc gggaataccc
     2641 ctgcagacct ttggatttca ttggatcaag accaaaggca atgctgtgca gcagcgtggc
     2701 cacaacggaa tagacggcgg ccgtcaccgg ttcatgcagc agcggcagga agccgaacag
     2761 gctggtcacc gccacggcgc cgccggtcag cagggatgcg aacagtttgc gtggaaaagc
     2821 cggcggtttg gccacttcgc gcgcgtcgta agcgtggtgg gctttttgtc cttccagcag
     2881 cagccaggcc gagagcaaca gcagggccag gccgccgaag cgtccggcca tttcgatggc
     2941 gctgccggtg atggcggaat acaggcccat gaacatcagc ggcagcggca ccaggaacat
     3001 ggagcgctcc cacagcatca ggcgggtttt gacgaaatgc cgcgccttgc ggaacagttc
     3061 ctcgggcgtg ggctccggcc gggcgttgcc ggggcccggg tggcgggcgt cgcgcaccgg
     3121 cggattcacg aagcgccccc gaacatgacg atcagcaaga ggattaccag caggaaaaaa
     3181 gccaatcccc gtatgaatac tccggccatg attctgtcct cgtggttgtg ctggcgtcac
     3241 cgtgactgcg agccattatg ccacaggcgc tgatggagga ggggtgatcg gttggtcacc
     3301 ggctgtgcgg ctggcgacgg ggcaaggcat tgccat
//
LOCUS       DASUEG010000418         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_632868, whole genome shotgun sequence.
ACCESSION   DASUEG010000418 DASUEG010000000
VERSION     DASUEG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632868"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFX11_07805"
     CDS             complement(<1..718)
                     /locus_tag="VFX11_07805"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938561.1"
                     /translation="MSLKSSSHESHKRAFITGAASGLGRELALRFARAGYRICVADIH
                     EARANAVVDEIKALGQDAFFYHCDVSRDQDLLAAATTMKERWGGTDVLINNAGVAGGG
                     PFDWLTLEDWHWMMNINFNGVLRGCHAFVPAMKEQGHGHIVNIASMAGLLNPPGMSNY
                     NVAKAAVVSLSETLAPELHPFGIGVTCVCPSFFRTNLGESLRSPDLSTQQTLNKLVDN
                     STDLSAADIADRIFTAVVNNK"
     gene            835..1698
                     /locus_tag="VFX11_07810"
     CDS             835..1698
                     /locus_tag="VFX11_07810"
                     /inference="COORDINATES: protein motif:HMM:NF027559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HEX4938562.1"
                     /translation="MLRAIKVLLCTVFLGSLATVTTANTLERIFTQANVVDERSEQNV
                     HYLLPLGRVKEDRKAGRAVPSHFERLQGNLVAVTWKLEGALSLLDARQLILAFLERQK
                     ADLMFECEARDCGESALWANGVFGQPLLFGSDRAQFMWVIRSREASRYHVLYLVERPN
                     RRLYFHEETLTVPAGEVSAAAAQTALKARGRVMVGRVPVLEGKANFADIVTRVVQWKE
                     TVSLPPVLVIHRHGETVAMTGLQQQLMEALTGAGVQAQVEDVGPLAPDVDAPGLIWVE
                     WVNTAWYPGQD"
     gene            1741..>2013
                     /locus_tag="VFX11_07815"
     CDS             1741..>2013
                     /locus_tag="VFX11_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006030276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX4938563.1"
                     /translation="MVTPIETADDLINLDEAIDELQTIRDFIRWAVSQFHAQGVTLGH
                     GYDDPWDEAVAIILHALHLPWDIDNRIQDARLLTSEKQRIAHQINRR"
BASE COUNT          370 a          594 c          643 g          406 t
ORIGIN      
        1 acttgttgtt caccaccgcc gtgaaaatac gatcggcgat atcggcggcg gacaggtcgg
       61 tcgagttgtc caccagtttg ttgagggtct gctgggtgct caggtcgggc gagcgcaggg
      121 attcccccag attggtgcgg aagaaggaag ggcacacgca ggtgacgccg atgccaaacg
      181 ggtgcagttc cggcgccagg gtttccgaca gcgaaacgac cgccgctttg gcgacgttat
      241 agttgctcat tcccggcggg ttcagcagcc cggccatcga cgcgatgttc acgatatggc
      301 cgtggccctg ttccttcatc gccggcacaa aggcatggca gccgcgcagc acgccgttga
      361 agttgatgtt catcatccag tgccagtctt ccagcgtcag ccagtcgaag gggccgccgc
      421 ccgccacgcc ggcgttgttg atcagcacat ccgttccgcc ccagcgttct ttcatggtgg
      481 tcgctgccgc cagcagatcc tggtcccggc tgacgtcaca gtggtagaaa aaggcgtcct
      541 ggcccaacgc cttgatttcg tccaccaccg cgttggcgcg ggcttcgtgg atgtcggcca
      601 cgcagatgcg gtagccggca cgggcaaagc gcaaggccag ttcccggccc aggccgctgg
      661 cggcaccggt gatgaaggcc cgcttgtggg attcgtggga gctgctcttg agggacatgg
      721 gttccttcct ctccatatgc aatgatgtga tggccgggca gcgggcaggc aggcgacttg
      781 aactgccatt aacgctttgt tagcgttgtg gtttccacta gggaaaggca ctcgatgctc
      841 cgcgcaatca aggttttact ttgtactgtt tttctgggct ctctggcaac cgttacaacg
      901 gccaataccc tggagcgcat cttcacccag gccaatgtgg ttgacgagcg cagcgagcag
      961 aacgttcact atctgctgcc cctgggccgg gtgaaggagg atcgcaaggc cgggcgggcg
     1021 gtgccgtctc acttcgaacg cctgcagggc aacctggttg cggtgacctg gaagctggaa
     1081 ggtgcgctgt ccttgctgga tgcacgtcag ctgattctgg catttctgga acggcaaaaa
     1141 gccgatctga tgttcgagtg cgaagcccgc gactgtggcg agagtgcgct gtgggccaac
     1201 ggcgtatttg gccagcccct gctgttcggt tccgatcgcg cccaattcat gtgggtgatc
     1261 cgttcccgcg aagcgtcgcg ttaccatgtg ctgtatctgg tggaacgccc caaccgccgg
     1321 ctttatttcc acgaagaaac ccttacggtg cccgctggtg aggtgagcgc cgccgccgca
     1381 caaaccgccc tgaaagcaag ggggcgggtg atggtcggcc gggtgccggt actggaaggc
     1441 aaagccaatt tcgccgacat agtcacccgg gtggtccagt ggaaggagac cgtttccttg
     1501 ccgccggtgc tggtgatcca tcgtcacggt gaaacggttg ccatgacggg attgcagcag
     1561 cagctgatgg aggccctgac aggcgccggc gttcaggccc aggtggaaga cgtggggccg
     1621 ctggcaccgg acgtggatgc gccggggctg atctgggtgg agtgggtcaa caccgcctgg
     1681 tatccgggtc aggattgagt ctgtacaatc gccgttttcc catagatgga tgaagcccgc
     1741 atggtgaccc cgatcgaaac cgccgacgac ctgatcaacc ttgacgaagc catcgacgaa
     1801 ctgcagacca ttcgcgattt catccgctgg gccgtgtcgc agttccatgc ccagggcgtc
     1861 accctcggcc atggttacga cgatccctgg gacgaagcgg ttgccatcat cctgcatgcc
     1921 ctgcatctgc cctgggatat cgacaaccgg attcaggatg cgcgcctgct caccagtgaa
     1981 aaacagcgca ttgctcacca gatcaaccgc cgt
//
LOCUS       DASUEG010000419         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1297437, whole genome shotgun sequence.
ACCESSION   DASUEG010000419 DASUEG010000000
VERSION     DASUEG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297437"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1463
                     /gene="gatA"
                     /locus_tag="VFX11_07820"
     CDS             9..1463
                     /gene="gatA"
                     /locus_tag="VFX11_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX4938564.1"
                     /translation="MHDKTLNGLADALATRQVSSVELTRHFLDRIKRFDPQLNSFITV
                     TEAQALEQAAAADAARAAGKAGRLTGVPIAHKDIFCTTGVKTSCGSKMLDNFIAPYNA
                     TVVEKMNSAGLVMLGKTNMDEFAMGSSNESSFYGAVKNPWNTDYVPGGSSGGSAAAVA
                     ARLAPAATGTDTGGSIRQPASLSGITGLKPTYGRVSRWGMIAFASSLDQAGPMAQTAE
                     DAAVLLNAMAGFDERDSTSTNIEVPDFTATLNDSLDGLRIGLPKEFFGAGLDAGVEKC
                     VQDAIREYEKLGAKIVEISLPNTHLSIPAYYVIAPAEASSNLSRFDGVRFGYRCKSPE
                     NLEDLYKRSRGEGFGDEVKRRILIGTYALSAGYYDAYYLKAQKVRRLIKNDFDAAFAK
                     VDVIMGPTAPHAGFKLGEKAANPVSMYLSDIYTISVNMAGLPGLSLPAGRADGLPVGL
                     QIIGNYFDEARLLNVAHRYQQVTDWHTQLPAAFA"
     gene            1485..2930
                     /gene="gatB"
                     /locus_tag="VFX11_07825"
     CDS             1485..2930
                     /gene="gatB"
                     /locus_tag="VFX11_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX4938565.1"
                     /translation="MQWETVIGLEVHVQLSTQSKIFSGSRNAFGAEPNTQASLIDLGM
                     PGVLPVLNQEAVHKAIKFGLGINAEIAPRSVFARKNYFYPDLPKGYQISQYELPIVGK
                     GFVDITLEDGTVKRIGVTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDM
                     RSAEEAVAYLKAIHSIIRYLDISDGNMAEGSMRCDCNVSVRLKGEEKFGTRREVKNVN
                     SFRFVEKAINVEAQWQIDELESGRQIQQCTVLYDSAKNETRAMRSKEEANDYRYFPDP
                     DLLPVAITENMINRMRATLPELPAQKIERFMGELELSEYDARVLAADRAVADYFEQVV
                     KESGAKPKQAANWVSGDLLGALNKNDLSIEQSPVSAAQLAGLIKRIVDNTISGKIAKQ
                     VFEELWSNGGTADSIIESKGLKQETDTGAIEAIVDQVIASSPDQVAQYLAADENKRKK
                     LVGYFVGQIMKLSKGKANPQLVNDILAKKLV"
     gene            3256..>3461
                     /locus_tag="VFX11_07830"
     CDS             3256..>3461
                     /locus_tag="VFX11_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938566.1"
                     /translation="MRAEAAEGFTFLGFMPDGDDTAYVTYGLVLVSGGVYGAPLKFFQ
                     PATADVHAVFYPTDQLTQRVSSGG"
BASE COUNT          742 a         1036 c         1030 g          653 t
ORIGIN      
        1 cgcattccat gcatgacaag acactcaatg gcctggcgga cgccctggcc acccggcagg
       61 tttccagcgt tgagctgacc cgccacttcc tggaccggat caagcgcttc gatccgcagt
      121 tgaacagctt catcacggtg accgaggccc aggcgctgga acaggccgcc gccgccgacg
      181 ctgcccgcgc tgccggcaag gccgggcgcc tgaccggcgt gcccatcgcc cacaaggaca
      241 tcttctgcac caccggcgtg aaaaccagct gcggctcgaa gatgctggac aacttcattg
      301 ccccctacaa cgccaccgta gtggaaaaaa tgaacagtgc cggcctggtg atgctgggca
      361 agaccaacat ggacgagttc gccatgggtt cgtccaacga atccagtttt tacggcgcgg
      421 tgaaaaatcc gtggaatacg gattatgttc ccggcggttc ctccggcgga tcggctgcag
      481 ccgtggccgc ccgcctggcc cccgccgcca ccggcaccga caccggcggc tcgattcgcc
      541 agcccgccag cctgagtggc atcaccggtt tgaagcccac ctatggccgc gtgtcgcgct
      601 ggggcatgat cgcgtttgcc tcgtcactgg atcaggccgg cccgatggcc cagaccgccg
      661 aagacgccgc cgtgttgctg aatgccatgg ccggctttga cgagcgcgat tccaccagca
      721 ccaacattga agtgcccgat ttcaccgcca cgctgaacga ttcgctggac ggtttgcgca
      781 tcggcctgcc gaaagaattt ttcggcgcgg gcctggatgc cggcgtggaa aaatgcgtgc
      841 aggacgcgat ccgcgagtac gaaaaactgg gcgcgaaaat tgtcgagatt tccctgccca
      901 acacgcacct gtcgattccc gcctattacg tgattgcgcc ggcggaagcc tcgtccaacc
      961 tgtcgcgctt tgacggcgtg cgttttggct accgctgcaa gtcgccggag aacctggaag
     1021 acctgtacaa acgcagtcgc ggtgaagggt ttggcgacga agtgaagcgg cggattctga
     1081 tcggcaccta cgcgctgtcg gccggctatt acgatgccta ttacctgaaa gcgcagaaag
     1141 tgcggcgcct gatcaagaac gatttcgacg ccgcgtttgc gaaagtcgat gtgatcatgg
     1201 gccccaccgc gccccacgcc gggttcaaac tgggcgagaa ggcggccaat ccggtgtcga
     1261 tgtatctgtc cgacatctac accatttcgg tgaacatggc ggggttgccg gggctgtcac
     1321 tgccggcggg ccgtgctgac ggtttgccgg tgggcctgca gatcatcggc aactatttcg
     1381 atgaagcgcg cctgctgaat gtggcgcacc gttatcagca ggtcaccgac tggcacactc
     1441 agttgccagc ggcctttgcg taattttctg aaggggtcac aaccatgcaa tgggaaaccg
     1501 tgatcggcct ggaagtgcac gtgcaacttt ccacgcagtc gaaaatcttt tccggcagcc
     1561 gcaatgcatt cggcgccgaa ccgaacacac aggccagcct gatcgatctg ggcatgcccg
     1621 gcgtgctgcc ggtgctgaac caggaggccg tgcacaaggc catcaagttc ggtctgggca
     1681 tcaatgctga aatcgcgccg cgttccgtgt tcgcgcgcaa aaactatttc taccccgacc
     1741 tgcccaaagg ctaccagatc agccagtacg aactgccgat tgtgggcaag ggctttgtcg
     1801 acatcacgct ggaggatggc accgtcaagc gcatcggcgt gacccgcgcg catctggaag
     1861 aggacgccgg caaatccctg cacgaggatt tccacggcat gagcggcatc gacctgaacc
     1921 gtgccggcac ccccctgctg gaaatcgtgt cggagccgga catgcgttcc gccgaggagg
     1981 cggttgcgta tctgaaagcc attcactcca tcatccgcta cctggacatt tccgatggca
     2041 acatggcgga aggctccatg cgctgcgact gcaacgtgtc ggtgcggctg aaaggcgaag
     2101 agaaattcgg cacgcggcgt gaagtgaaga acgtcaactc gttccgcttc gtggagaagg
     2161 ccatcaatgt ggaagcacag tggcagatcg acgagctgga atccggccgc cagatccagc
     2221 agtgcaccgt gctgtacgac tcggcgaaaa acgaaacccg cgccatgcgc agcaaggaag
     2281 aagcgaacga ttaccgctac ttccccgatc cggatctgct gccggtggca atcaccgaga
     2341 acatgatcaa ccgcatgcgc gccacgctgc cggaactgcc ggcacagaaa atcgagcgtt
     2401 tcatgggcga gctggaactg tcggaatacg atgcccgtgt actggcagcc gatcgcgcag
     2461 tggcggatta tttcgagcag gtggtgaagg aatccggcgc caaaccgaag caggcggcga
     2521 actgggtgag cggcgatctg ctgggcgcgc tgaacaagaa tgatctgtcc atcgagcaga
     2581 gtccggtaag cgcggcgcaa ctggcagggc tgatcaaacg catcgtcgac aacaccatct
     2641 ccggcaagat cgccaagcag gtgtttgagg aactgtggag caacggcggc acggcggatt
     2701 cgatcattga aagcaagggc ctgaaacagg aaaccgacac cggcgcgatt gaagcgattg
     2761 tggatcaggt gattgcgtcc agcccggatc aggtggcgca gtatctggcg gcggatgaaa
     2821 acaagcgcaa aaaactggtg ggctatttcg tcggacagat catgaagctg tcgaagggca
     2881 aggccaatcc gcagctggtg aatgacattc tggcgaaaaa actggtttaa gctctcctct
     2941 ttaattctcc ttcacgcacc tgaagtcagc ctgtatggcg tcaaatacat tctccccccc
     3001 ccatccgttc atccggtccc gcttttcatc gccattcaac cgcgctagcg tagcttcgca
     3061 aacaacaatc acatggatgg tgacctatga ataatctgac caggttatgt tgcgccgcta
     3121 tggcgttgtt actggcggga tgcaatctcg acatcgacat tgacggacaa gggcgggttt
     3181 attcaaccga gggggggtat tgattgcagc tccgattgcc gtgacaccaa caacggcagc
     3241 cccgtatcca ccacgttgcg cgccgaagct gcggagggtt ttacctttct gggattcatg
     3301 ccggatggtg acgacactgc gtatgtcacc tacggacttg tcctcgtcag cggcggggtc
     3361 tatggcgcac ctctgaaatt ttttcaacct gctaccgccg atgtgcatgc cgtgttttat
     3421 cccaccgacc agctgaccca gcgcgtcagc tccggtggca g
//
LOCUS       DASUEG010000420         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_747717, whole genome shotgun sequence.
ACCESSION   DASUEG010000420 DASUEG010000000
VERSION     DASUEG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747717"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VFX11_07835"
     CDS             <1..1567
                     /locus_tag="VFX11_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4938567.1"
                     /translation="GLTQLKGRQVFIKGIVGIFSTEMELGGYGKYMTDMIENYRQQHT
                     AFEDENGDLLYGRSPWDEYVYNETLHTKSKSEGGPTLMLPGGMIDMPIGAGIAGGASY
                     SPPGSRFTFGTNVYSPMMNGMHVAPDDPGRFAQERASFTLITYFSPTVAYEVSDELSV
                     GLSVNFNYSGMGLELPIHEPHVAIFFLGSPFIQGTFCNPDGTPKQVGDNSFGIDPSAV
                     VDVCTEVPPFTTFGKLSFEVDQNIVLGYNLGLLWKPTPWMSLGLSYNSSVNVEMEGEF
                     SFPIYDPFKTFLYNFHSSDVIEAVGGITGALGMPLPSPEEIKNGAEGPLTVSYELPQR
                     WNAGLSLQVLPNWKWNIDARWTEWSAFSQIKLDFGVDVALLKWGAIADLVGTGGQNGI
                     TPNSVDYQISLRDVAYWGTGVEYQFSDHLVLRGGFEDRPSAVPEDAPNALIPLNDAKL
                     FSLGFGLDLNSGSHIDFAVAHMKSETDFPACSAALGNGCNPANVVYPSYAGQDIKTKV
                     EILVFELGYQRQF"
     gene            complement(1613..>2521)
                     /locus_tag="VFX11_07840"
     CDS             complement(1613..>2521)
                     /locus_tag="VFX11_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4938568.1"
                     /translation="TVPLVLDEDELPQGHPAPASASEPVTFHYTQQKPLAVRVDRNVR
                     VTDSASDTDIHHIVLDFQNTDFTWLEGQNIGVLPPGEDAHGHAHHMRAYSIASSRGGD
                     TVGSRQMALTIKRVIDVQDGKPYYGIASNYMCDLKPGDSVRCVGPIGSHFLMPQDPAS
                     RILMICSGTGIAPMRSFVQQHQRIDDNTRVPLQLFYGGRTESEMAYVDELRQVPEGTL
                     ALHLALSRSAVHPKRYVQDLLRERSDQVAEFLQDRNGHIFICGMIAMGQAVMSLLAEI
                     AEQHQLRWKDLHHKLLHEGRLQIETY"
BASE COUNT          562 a          656 c          694 g          609 t
ORIGIN      
        1 cggtttgacg caactgaaag gcaggcaggt ttttatcaag ggaattgtgg gcatattctc
       61 cactgaaatg gaattgggag ggtatggcaa atacatgacg gatatgattg agaattaccg
      121 gcagcaacac acggcgttcg aagatgaaaa tggtgatctg ctatatggca gaagcccctg
      181 ggacgagtac gtctacaacg agacgcttca tacaaaaagt aaatcagagg gaggccctac
      241 cctgatgctg ccgggtggga tgatcgatat gccgattggt gctggaatcg caggaggagc
      301 ttcatacagc ccaccgggca gtcgcttcac ctttggtacc aatgtgtatt cgcccatgat
      361 gaatggaatg cacgttgctc ctgatgaccc tggccggttt gcgcaagagc gtgcttcgtt
      421 cactctcata acctatttct cgccaacggt tgcatacgaa gtatcagatg aactttctgt
      481 cgggctttca gttaatttca actactccgg catgggattg gaattgccaa ttcatgaacc
      541 tcatgtcgcc atattttttc ttggctctcc ctttatccag ggcacattct gcaatcctga
      601 cggaacaccg aaacaggtgg gtgacaattc ttttggaatt gatccgtctg cagttgttga
      661 tgtttgtact gaggttcccc ccttcacgac gtttggaaaa ctgtcgtttg aagtcgatca
      721 gaacattgta cttggctaca acctggggct gctgtggaag cccacgccct ggatgtccct
      781 ggggctttcc tacaattcca gcgtgaatgt cgagatggaa ggtgagtttt cttttcccat
      841 ttacgatcca ttcaaaacat tcctttacaa ttttcacagc agcgatgtga tagaggcggt
      901 gggcggaatt acgggcgctc tagggatgcc gttaccttca ccggaagaaa tcaagaacgg
      961 tgccgaagga ccgctaacgg tttcgtacga actgccccaa cgctggaatg caggattaag
     1021 cctccaagtc ttgcccaact ggaaatggaa tatcgatgcc cggtggacgg agtggtcggc
     1081 gttcagccag atcaagctcg actttggtgt cgacgtcgcc ttgctgaaat ggggtgccat
     1141 agcggatctg gtaggaaccg gcggtcaaaa cggcattaca cccaactctg tcgactacca
     1201 gatcagtctg cgcgatgtgg cttactgggg aaccggtgtg gagtatcaat tcagtgatca
     1261 tctggtattg cgtggtggat ttgaggaccg gccatctgcc gtgccggaag atgcccccaa
     1321 tgcattgatc ccgctgaatg acgcgaaact gttttcactc ggattcggcc tggatctgaa
     1381 ctccggtagc catatcgatt ttgcggtggc tcacatgaaa tccgaaacgg actttccggc
     1441 ctgttcagcg gcactgggaa atggctgcaa ccctgccaat gttgtatatc cctcttatgc
     1501 gggacaggac attaaaacga aagttgagat tcttgtattt gagcttggtt accagcgaca
     1561 gttctagatt tccgacttca ttgcgggctc tggcacacct cgacagacaa catcaatagg
     1621 tttcgatttg caggcgcccc tcgtggagca gtttgtggtg cagatctttc caccgcaatt
     1681 ggtgctgttc tgcgatctcg gccagcagac tcatcaccgc ctgacccatc gcgatcatgc
     1741 cgcaaatgaa gatatggcca ttccggtcct gcagaaattc cgccacctga tcgctgcgct
     1801 cccgcagcag atcctgcacg taacgttttg gatgcaccgc gctgcgcgat agtgccagat
     1861 gcagcgccag cgtcccctcc ggtacctgcc gcagttcgtc cacatacgcc atctcgcttt
     1921 cggtgcgccc gccgtagaac aactgcagtg gaacccgggt attgtcgtca atgcgctgat
     1981 gctgctgcac gaacgagcgc atgggtgcga tgccggtacc cgaacagatc atcaagatgc
     2041 gactggcggg gtcctgcggc atgaggaagt gggaaccgat ggggccgacg cagcgcacgc
     2101 tgtcccccgg tttcagatcg cacatgtaat tggacgcgat gccgtaatag ggtttgccgt
     2161 cttgcacatc aatgactctt ttgatggtca gtgccatctg ccggctaccg acggtatcgc
     2221 cgccgcgact gcttgcaatg gaataggccc gcatgtgatg ggcgtgacca tgggcatcct
     2281 cacccggtgg cagcacaccg atattctggc cttccagcca ggtgaaatcg gtattctgaa
     2341 aatccagaac gatgtggtga atgtcggtgt ccgacgcaga atccgttacg cgaacattgc
     2401 gatcgacgcg cacggcgagc ggcttttgct gggtataatg gaacgtgacg ggttcactgg
     2461 cggacgctgg cgccggatgt ccctgtggca actcatcttc atccagcacc agtggcacag
     2521 t
//
LOCUS       DASUEG010000421         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_817923, whole genome shotgun sequence.
ACCESSION   DASUEG010000421 DASUEG010000000
VERSION     DASUEG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817923"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFX11_07845"
     CDS             <1..782
                     /locus_tag="VFX11_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938569.1"
                     /translation="LPLDGRSDLYSLGVVLVEMLSGSNPFRGKDYGTTVVNQLQMAAP
                     RLPAGQQHWQPIVDRLLAKHPQDRYADAAELLAAVGGEAGSALAVAEGVGADTDATSV
                     RAVLPASGGQEPLWRNRRVQVGAGVLLLILMLVGWWAGREDPQVAEWLVKAGQRLEND
                     QLNSPAGDNAVFYYRLVLKRDADNRKAQKGLNLVAARYAELARTARDEQNFRMALEYV
                     ERGLAIRPDDADLLALDAEIREQVRANRKGLGKFLDGLFGQ"
     gene            943..2805
                     /locus_tag="VFX11_07850"
     CDS             943..2805
                     /locus_tag="VFX11_07850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938570.1"
                     /translation="MTRTRTFAALLLFLLLWPLQGVRAAVSLADGQVSRPVTDALQYF
                     QDDSGRMGLEQALAMPASRWQQNGPQTFSHGYTPSHWWLRFQVDNPQAQPARWLLELA
                     YPVLDHVEVWILSPEGRVEQHHLLGDKQPFHERLLQHRFFLIPLDLSAASGRTVVMRL
                     HSSSSMQAPLTLWEERAYHEQDQHVLLVQGLFFGGMALMALYSFFVFIALRDRLYFYY
                     VVFVFCVLGFLASLKGLSFQFLWPQATAWNDRVLVVTLALMMSSGGLFTLRFLRLRQY
                     MPRYFWLALGLVLLAGAHAVMAFFRDYATLMRPLIVIAAISCLVFLSGGVWRWSQGDR
                     SARFYSIAWSSMLLGGVVLAMNKFQWLPQNFITENAMQLGVAAELILLSLAIVDRINE
                     DRRQRHKAQQEIFQSERRAYEAQAKALAAEKEANERLEQRVNERTLALQEANLKLEEL
                     STSDQLTGLRNRRYLDRVLQEECCRSHRYRHSMAVLLLDVDHFKRFNDQWGHDAGDEC
                     LRRVAAVLAISVRNPVDHVARYGGEEFCVVMPETDAAGAYAVAERIRQGVESMEFMIG
                     DMRVPVTISVGIMACVPPDENYSREFLKQADIMLYQSKGAGRNCVSLSEAVSQG"
     gene            complement(2822..>3407)
                     /locus_tag="VFX11_07855"
     CDS             complement(2822..>3407)
                     /locus_tag="VFX11_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4938571.1"
                     /translation="RTTVDKTAKLLAEMGHEVEEMPMPIDPVFISDFSTYWGMLSFLV
                     STFGRKVISPDFDASRIDNLSRGLASLYRKNFLKTPMVLYRLKKTLDDYARVFDTYDL
                     VLSPVLAHTTPKIGYLSPEQDFEQLFDRLMKYVSFTPINNASGSPAISLPMGHSALGL
                     PIAVQLSATNGDERTLLEVAYALEEAHPWRRIQD"
BASE COUNT          567 a          924 c         1184 g          732 t
ORIGIN      
        1 tgttgccgct ggatggtcgc agcgacctgt acagcctggg cgtggtactg gtggaaatgc
       61 tgtcggggag taatccgttc cgcggcaagg attacggaac gaccgtggta aaccagttgc
      121 agatggccgc tccgcgcttg ccggctggcc agcagcactg gcagcccatt gtcgaccgct
      181 tgctggccaa acatccgcag gaccgctatg ccgacgctgc ggagttgctg gccgctgttg
      241 gcggtgaggc gggttcggca ctggcggtgg ccgaaggtgt tggggccgat acggacgcca
      301 cttcggtgcg tgcggtactg ccggcatccg gtgggcagga gcctttgtgg cggaatcgtc
      361 gtgtccaagt gggggcgggg gtgctgttgc tgatactcat gctggtgggg tggtgggcgg
      421 gacgggaaga tccgcaggtg gctgagtggc tggtcaaggc cgggcagcgg ctggagaacg
      481 accaactgaa cagtcctgcc ggtgacaatg cggtgttcta ttaccggctg gtgctcaagc
      541 gcgatgcgga taaccgcaag gcacagaagg ggctgaatct ggtggcggcc cggtatgccg
      601 agctggcgcg caccgccagg gatgagcaga acttccggat ggcgctggag tacgtggagc
      661 gagggttggc aattcgcccg gatgatgccg acctgctggc gctggatgcc gaaattcgtg
      721 agcaggtgcg ggcgaatcgc aaggggttgg gcaagtttct ggatgggctg ttcgggcagt
      781 agcggaatgt cgggtgtggg gttagtccgt cgctgccagg atactgctgg caggctgaac
      841 taacgggacg accggtcaga cgcgcccctc gtcggagatg acttgtcgtc ggaggcctgt
      901 tgttgcgaac gttgggccaa taacagtcct gcgacggtca gaatgacaag aacaagaacg
      961 ttcgccgctc tcctgctttt cctgctgctg tggccgctgc aaggcgttcg tgccgcagtg
     1021 tcgctggcgg atgggcaggt gtcgcgtccg gtcacggatg cccttcagta tttccaggat
     1081 gactcgggcc gcatgggcct tgagcaggcc ttggccatgc ccgcgtcgcg ctggcagcag
     1141 aatggcccgc aaacctttag tcatggctat acgccgtcgc actggtggtt gcgctttcag
     1201 gtggataacc cgcaggcgca gccggcccgc tggctgctgg agctggccta cccggtactg
     1261 gaccatgtcg aggtctggat tctctcgccg gaaggccggg tagagcagca ccaccttctg
     1321 ggggacaagc agcccttcca cgagcgtttg ttgcagcacc gattcttcct gattccgctg
     1381 gacctgtcgg ccgcgagtgg gcggacggtg gtcatgcgtt tgcattcgtc cagctccatg
     1441 caggctccgc tgacgctgtg ggaggaacgc gcctatcacg agcaggatca gcacgtgctg
     1501 ctggtgcagg gcctgttctt tggcggcatg gcactgatgg cgctgtacag cttctttgtc
     1561 ttcattgcct tgcgtgaccg cctgtatttt tactacgtgg ttttcgtgtt ctgtgtgctg
     1621 ggtttcctgg ccagcctgaa gggcctgtcc ttccagttcc tgtggccgca ggccacggcc
     1681 tggaatgacc gtgtactggt ggtgaccctg gcgctgatga tgtcttcggg tgggttgttc
     1741 acgctgcgct tcctgcgcct gcgccagtac atgccgcgct atttctggct ggcgctgggg
     1801 ctggtgttgc tggccggggc gcatgccgtg atggccttct tccgggacta tgccacgctc
     1861 atgcggccgc tgatcgtgat tgcggcgatc tcctgtctgg tctttctgtc cggtggtgtc
     1921 tggcgctgga gtcagggcga ccgctctgcg cgtttctatt cgattgcctg gagctccatg
     1981 ctgctggggg gcgtggtgct ggcgatgaac aagttccagt ggctgccgca gaatttcatt
     2041 accgaaaacg cgatgcagct gggcgtggcg gcggagctca tcctgttgtc attggctatc
     2101 gtggaccgca tcaacgaaga ccgtcgtcag cgtcacaagg cccagcagga aatcttccag
     2161 tcggagcgcc gggcctacga ggcgcaggcc aaggcgctgg cggccgagaa ggaagccaac
     2221 gagcggttgg agcagcgcgt gaacgagcgc acgctggcgc tgcaggaggc caatctcaag
     2281 ctggaggagc tgagtacttc ggaccagctg accgggttgc gcaatcgtcg ttatctggat
     2341 cgcgtgctgc aggaagaatg ctgccgcagt catcgctacc ggcactcgat ggcggtgttg
     2401 ttgctggatg tggatcactt caagcgcttc aacgaccagt ggggacatga cgcgggtgac
     2461 gagtgcctgc gtcgtgttgc ggcggtgctg gccatttcgg tgcgcaatcc tgtggatcat
     2521 gtggcacgct atggcggtga agagttctgt gtggtgatgc ccgagacgga cgcggccggt
     2581 gcctatgccg tggccgagcg catacggcag ggggtcgaga gcatggagtt catgattggt
     2641 gacatgcgcg tgccggtcac catcagcgtg ggcatcatgg cttgtgtgcc gccggacgag
     2701 aattacagcc gcgagttcct caagcaggcc gacatcatgc tgtaccagtc caagggcgcg
     2761 ggtcgcaact gcgtgtcact gagcgaggcg gtcagtcagg gctgaccgcc agccgtccgg
     2821 cttagtcctg gatgcgtcgc cagggatgag cctcttccag tgcgtaagcc acttccagca
     2881 gcgtgcgctc gtcgccgttg gtggcagaga gctgcacggc aatcggcaat cccagcgcag
     2941 agtgacccat cggcagtgaa atggccgggc tgccactggc gttgttgatc ggcgtgaagc
     3001 tcacgtactt catcaggcgg tcaaacagct gctcgaaatc ctgctccggt gagagatagc
     3061 cgattttcgg tgtggtgtgc gccagtaccg ggctcagtac cagatcgtag gtgtcgaaca
     3121 cacgggcgta gtcgtccagg gttttcttca ggcgatacag caccatcggt gttttcagga
     3181 aattcttgcg gtagagcgag gccagtcccc ggctcaggtt gtcgatacgc gaggcatcaa
     3241 aatcggggct gatcaccttt cggccaaagg tgctgaccaa aaaggacagc atgccccagt
     3301 aagtgctgaa atcgctgatg aaaaccggat cgatgggcat gggcatttct tcgacctcat
     3361 gtcccatctc tgcgagcagc ttggctgttt tgtcgaccgt tgtgcgt
//
LOCUS       DASUEG010000422         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_332618, whole genome shotgun sequence.
ACCESSION   DASUEG010000422 DASUEG010000000
VERSION     DASUEG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332618"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VFX11_07860"
     CDS             <1..1276
                     /locus_tag="VFX11_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX4938572.1"
                     /translation="GLLRRDLKLPDFRDYAVSVPVAGAVRYENTRPLGEFLRDVMRNN
                     LTSFRVFGPDETASNRLQALYEVSKKTWMADLLPEDADGGELSRDGRVMEMLSEHTLL
                     GWFEGYLLTGRHGFFHTYEAFAHVVDSMFNQHAKWLDISKNHVPWRAAVASQNILLSS
                     TVWRQDHNGFSHQDPGFIDVVTNKSPSVTRIYLPPDANTLLVTADQCLRSTDCINVIV
                     SDKQKHMQFTTIDEAVVHCAKGIGIWRRASNDEGVEPDVVLAACGDVPTLEALAAVAI
                     LRERLPDLKLRFVNVVDLFRLQPETEHPHGLTPREFDSLFTTNKPIIFNFHGYPWLIH
                     KLAYRRTNHANLHVRGYKERGNINTPLELAILNQIDRFNLVIDVIDRVPGLAARAAHL
                     KEEMKNAIIDNLNYAHTHGTDREDISNWVWPY"
     gene            1292..1882
                     /locus_tag="VFX11_07865"
     CDS             1292..1882
                     /locus_tag="VFX11_07865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007098238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4938573.1"
                     /translation="MSKRHIYLIRHGETEWSLNGRHTGRTDIPLTDNGRAVALGLAPV
                     LAKVQETAPFDLVLSSPLQRARETCELAGLGAQAQVDPDLVEWNYGDYEGLTPQQIRA
                     GHSDWLLFRDGCPNGETPEHVGARADWVISRARACKGHVAIFAHGHFLRVLGARWIGL
                     QASGGAHFLLDTATLCVLSEYHGNAAILHWNAPIAT"
     gene            1921..>2127
                     /locus_tag="VFX11_07870"
     CDS             1921..>2127
                     /locus_tag="VFX11_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX4938574.1"
                     /translation="MSTQDNPTSTPATVSLADEDAVRRLLTDTLFGLWATVNNLSRLR
                     PSKRERYRVSVFGSARPQPEHWVYQ"
BASE COUNT          426 a          606 c          667 g          428 t
ORIGIN      
        1 cggcctgctg cgacgcgatc tgaaactgcc ggattttcgc gattacgcgg tgtcggtacc
       61 cgtggccggt gcggtgcgct atgaaaatac ccgtccgttg ggagaattcc tgcgcgacgt
      121 gatgcgcaac aacctgacca gcttccgcgt gttcgggccg gacgaaacag cgtccaaccg
      181 tttgcaggcg ttgtacgaag tgtcgaagaa aacctggatg gcggatctgt tgccggaaga
      241 tgccgacggc ggcgagctgt cgcgcgatgg ccgggtaatg gagatgctgt ctgagcacac
      301 tctgctgggc tggttcgaag gttatctact gacggggcga catggatttt tccacaccta
      361 tgaagcgttt gcgcatgtgg tggattccat gttcaaccag cacgccaagt ggctcgatat
      421 ttcgaaaaat catgtgccct ggcgcgcggc ggtggcgtcg cagaatattt tgttgtcgtc
      481 cactgtgtgg cggcaggatc acaacggttt ttcccaccag gacccgggct tcatcgatgt
      541 ggtgaccaac aagagtccgt cggtaacgcg gatctatctg ccgccggacg cgaatacatt
      601 gctggtcacc gctgatcagt gtctgcgttc cacggattgc atcaacgtga tcgtgtcgga
      661 taaacagaag cacatgcagt tcaccaccat tgacgaggcc gtggtgcatt gtgccaaggg
      721 tatcggcatc tggcgtcgtg ccagcaatga tgaaggcgtg gaaccggatg tggtgctggc
      781 cgcctgcggc gatgtgccca cactggaagc gctggcggcg gtggcgatcc tgcgggagcg
      841 gctgccggat ctgaaactgc gcttcgtcaa cgtggtggat ctgttcaggc tgcagccgga
      901 aacggaacat ccacacggcc ttacccctcg ggaattcgac agcctgttca ccaccaacaa
      961 gccgatcatt ttcaatttcc acggctatcc ctggctgatt cacaagctgg cgtatcgccg
     1021 caccaatcac gccaatctgc acgtgcgcgg ctacaaggaa cgcggcaata tcaacacacc
     1081 gctggagctg gcgatcctga accagatcga ccgtttcaat ctggtgatcg acgtgatcga
     1141 tcgggtgccg ggtctggcgg cgcgggcagc gcatctgaag gaagaaatga agaatgccat
     1201 catcgacaac ctgaactatg cgcacacgca tggtacggat cgtgaagata tcagcaactg
     1261 ggtgtggcct tattgagaaa gggagaagta catgtcgaag cggcatattt atctgatccg
     1321 tcacggtgaa accgagtgga gtctgaacgg gcgccatacc ggccgcaccg atattccgct
     1381 caccgacaat ggccgcgcgg tggcgctggg gctggcaccg gtgctggcga aagtgcagga
     1441 gacggcgccg ttcgatctgg tgctgtccag tccgttgcag cgggcgcgcg aaacctgcga
     1501 gctggcaggg ctgggcgcgc aggcgcaggt agatccggat ctggtggaat ggaattacgg
     1561 tgattatgaa gggctgaccc cacagcagat tcgcgccggc cattccgact ggctgctgtt
     1621 tcgcgatggc tgccccaatg gagaaacgcc ggaacatgtg ggcgcgcgcg cggactgggt
     1681 gatttcccgc gcccgtgcct gcaaagggca tgttgcgatt ttcgcccatg gccattttct
     1741 gcgggttttg ggcgcgcgct ggatcggcct gcaggccagc ggcggcgccc atttcctgct
     1801 cgacactgcc accctgtgcg tgctgagcga ataccacggc aatgccgcga tcctgcactg
     1861 gaacgcaccg attgccacct gacgcgcagc atcgaattta ccacaaggaa aacgcattcc
     1921 atgtccacgc aagacaatcc cacttctaca cccgccactg tcagcctcgc cgatgaagat
     1981 gcggtgcgac gcctgctcac cgatactctg ttcggcctgt gggccaccgt gaacaacctg
     2041 tcgcgcctgc ggccgtcgaa gcgggaacgt taccgggtgt cggtgttcgg ttcggcgcga
     2101 ccgcagccgg agcactgggt gtatcag
//
LOCUS       DASUEG010000423         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_504345, whole genome shotgun sequence.
ACCESSION   DASUEG010000423 DASUEG010000000
VERSION     DASUEG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504345"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFX11_07875"
     CDS             complement(<1..431)
                     /locus_tag="VFX11_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX4938575.1"
                     /translation="MKIRIACLALITLLHGCAADTAPTATSPADAAANSAVNAADALF
                     APASWTLVELQGKAVPKASDAHRAPFISFSREGNRVSGFAGCNRFTGTFTVESGNRLK
                     FSPLAMTRMMCADDVLEPEFMKVLETHERFQVEGEALVLSGA"
     gene            complement(501..1160)
                     /locus_tag="VFX11_07880"
     CDS             complement(501..1160)
                     /locus_tag="VFX11_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4938576.1"
                     /translation="MSMQLYAHPFSSYCQKVLIALYENNTPFQFRMLGPGEDSAAAEH
                     AALWPLKRMPLLVDAGRTVPEASIIIEYLGLYHPGPVQLIPADARAALDVRLLDRFFD
                     NYIMTPMQKIVFDTLRAPDNRDPAGVADARALLETAYRWLDSQLGAREWAAGAAFTLA
                     DCAAAPSLFYADWVHPVGADCANVRAYRQRLLARPSFARAVDEARPYRSFFPPGAPDR
                     D"
     gene            complement(1270..1512)
                     /locus_tag="VFX11_07885"
     CDS             complement(1270..1512)
                     /locus_tag="VFX11_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938577.1"
                     /translation="MRKTIGYLLQILGWGGAAWLGLVGGSFSAIYLMGFIGTGGREAG
                     GELALMLGITVAGVLIGIGLGRWGRRLVRQANAFKA"
     gene            complement(1563..2051)
                     /locus_tag="VFX11_07890"
     CDS             complement(1563..2051)
                     /locus_tag="VFX11_07890"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4938578.1"
                     /translation="MDLHRDWDVIKSEFATALNASRHCAIGTVGADGNPHITPIGFVF
                     MRDDKSLYYFEQYSKKLPLNYQTNRNVCVMVVNSAPSFWLSALVKGRFASFPGLRLYG
                     EVGEARQASAEEREALRQRIGPLKFLSGARKIWSGLETVRDIRLQGVEPVRYPRMMEH
                     LL"
     gene            2395..>3382
                     /locus_tag="VFX11_07895"
     CDS             2395..>3382
                     /locus_tag="VFX11_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938579.1"
                     /translation="MKTIQRLQRRVAGLLLPLAAAISTTLAPPAQSAIIGADGGITLY
                     ISYDNLDAVGGASWNDMITQVNTMTGASCTPTNNARAAQGAPSSPGSCPAGDTCLGRE
                     KLEGDIQRFAEYIFEATEGRHYLRRVYLADNGRAWTEADIRWNVGSGGSAAPYGWNVP
                     GAALGLNSSARRCIHDVVHHEFGHYFYHLPDKYARSDGYYQGTISGGAQFAVSVDVGD
                     NESVMSGNFPHRYSDPSNSSITLTYTPPGDPIVSGQMLTPAVLTDADPNNNGPDRAHH
                     GYTHPFAQDDWARLPTIHPDLVGAHTEGNFPAPDLAMMPPVDIRFLGEDEPFP"
BASE COUNT          740 a         1130 c          939 g          573 t
ORIGIN      
        1 gccccgctca gcaccagcgc ctcaccctcc acctgaaaac gctcatgggt ttccagcact
       61 ttcatgaatt ccggttcgag gacatcgtca gcacacatca tgcgggtcat ggccaggggc
      121 gagaatttca accggttgcc ggattccacg gtgaaagtgc cggtgaaacg gttgcaaccg
      181 gcgaatccag aaacgcgatt gccttcacgg ctgaagctga tgaacggtgc ccgatgcgcg
      241 tccgacgctt tcggcacggc cttgccctgc aattccacca gggtccagga tgccggagca
      301 aacagggcat cagcagcatt gacggcgcta ttggcagcgg catctgccgg cgatgtcgct
      361 gttggcgccg tgtctgcggc gcagccgtgc agcaacgtaa tcaaggcaag acaggcaatc
      421 cgaattttca tgaacacatt cctttcgtct ggggcatcgg ggttgatcat cggacactgg
      481 attgaagaga aacagcactc tcaatcccga tccggcgcgc cgggcggaaa aaaagaacgg
      541 tagggtcgcg cctcatccac cgcccgggcg aacgacggcc gcgccagcaa ccgctgccgg
      601 taagcacgta cattagcgca atccgccccc accggatgta cccagtccgc ataaaacagt
      661 gacggtgccg cagcgcaatc cgccagcgta aacgccgcgc ctgccgccca ctcccgcgca
      721 cccagttgtg aatccagcca gcggtaagcc gtttccagca gggcccgcgc gtccgccaca
      781 ccggcaggat cacgattatc cggcgcacga agggtgtcga acacgatttt ctgcatgggc
      841 gtcatgatgt agttgtcgaa aaagcggtcc agcaggcgta cgtccagggc ggcacgggca
      901 tccgccggaa tcagctgcac cgggccggga tgatacaggc cgagatattc gatgatgatg
      961 ctggcttccg gcaccgtgcg gccggcgtcc accagcaggg gcatgcgttt cagtggccac
     1021 agcgcagcat gctcggcggc agcgctgtcc tcacccggac ccagcatgcg gaactggaac
     1081 ggcgtgttgt tttcgtaaag cgcaatcagc accttctggc aataggatga aaagggatgg
     1141 gcgtacagct gcatggacat gaggaatctc ccgggaggcg tggtgccggc ataaccggca
     1201 cggatttcgt cgtgatcact gaattgtcgt tatggcgtgc ggcaaatcga cagggtcgcc
     1261 ggcagaaatt catgctttga acgcattggc ctggcgcacc aggcgccgac cccaacgccc
     1321 cagcccgatt cctatcagaa cgcccgccac cgtaatcccc agcatcagtg ccagttcgcc
     1381 gccagcctcc cggcccccgg tgccaataaa gcccatcaaa tagatggcgg aaaagctgcc
     1441 acccaccaat cccagccagg ccgcgccgcc ccaacccagg atctgcagca gatagccgat
     1501 ggtcttgcgc ataggacccg ctcccgttcg tatgacgccc gtcatcgacg tcgaaaacgc
     1561 acctacaaca gatgctccat catacgagga tagcgcacgg gctccactcc ctgcaggcgg
     1621 atatcgcgca cggtttccag gccagaccag atcttgcggg ctccgctgag gaatttcagc
     1681 ggcccgatcc gctgccgcag cgcttcccgt tcctccgccg aggcctgccg ggcttcaccc
     1741 acctcaccgt acaggcgcag gccggggaac gatgcaaagc ggcctttcac cagcgccgac
     1801 agccagaagg aaggagcgct gttcaccacc atcacgcaga cattgcggtt ggtctgatag
     1861 ttcagcggca gtttttttga atactgttca aagtaataca gcgacttgtc atcacgcatg
     1921 aacacgaaac cgatcggcgt gatatgcgga ttgccatcag cccccacggt accgatggca
     1981 caatgacggg acgcattcag cgccgttgca aattcggatt tgatgacgtc ccaatcccga
     2041 tgaagatcca tgaaaactcc ctgacagtga tcggcggaca aaagcaaaaa aatttgcagc
     2101 gcccgtggtg acagcactgc atttgacggc agaagcggca ttagtgccac gaccacgacg
     2161 atactatttc aaattcccac aactgccaaa aacaaatcgg caccctgcac cttttccggc
     2221 agcgacaacc acaccggcaa agcccgcatt cctgcgcaga atccgttgca accatgcccg
     2281 caaaataccg tcgaggccgg catcgaccgc gcgcccgctg cagtccgcgc ggatgagttg
     2341 gaagccggtg ccggcccacc actctgtcac ttgctcagca aagggacctg tctcatgaaa
     2401 acaatacaac gactgcagcg ccgcgtggcc ggcctgctgc tgccgctggc agcggccatc
     2461 agcaccaccc tcgcgccacc ggcccagtct gccatcatcg gcgccgacgg cggcatcacg
     2521 ctttatattt cctacgacaa cctggatgcc gtcggcggcg ccagctggaa cgacatgatc
     2581 acccaggtga acacgatgac cggcgccagc tgcacgccca ccaacaatgc acgtgctgca
     2641 cagggagccc ccagcagccc cggttcctgt ccggcgggtg acacctgcct gggccgggaa
     2701 aaactcgaag gcgatatcca gcgctttgcc gaatacattt tcgaagccac cgaaggccgc
     2761 cactacttgc gccgcgtata cctggcagac aacggccgcg cctggaccga agcggatatc
     2821 cgctggaacg tgggcagcgg cggctctgcc gcaccctatg gctggaacgt tccgggcgct
     2881 gcactgggcc tcaacagcag tgcgcgccgc tgcattcacg atgtggtgca ccacgagttt
     2941 ggccactact tctatcatct gccggacaaa tatgcgcgct ccgacggcta ctatcagggc
     3001 accataagcg gcggcgccca attcgccgtg agcgtcgatg tgggcgacaa cgaatccgtg
     3061 atgtccggca actttccgca tcgctattcc gatcccagca attccagcat taccctcacg
     3121 tacacgccac cgggcgatcc catcgtatcg ggccagatgc tgacacccgc cgtgctgacc
     3181 gacgccgatc ccaacaacaa cggacccgac cgcgcccatc acggctacac tcacccgttc
     3241 gcgcaggacg actgggcacg tctgcccacg atccatccgg atctggtcgg cgcccacacc
     3301 gaaggcaatt ttcccgcgcc ggatctggcc atgatgccgc cggtggatat ccgttttctg
     3361 ggagaagacg aacccttccc cg
//
LOCUS       DASUEG010000424         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_331375, whole genome shotgun sequence.
ACCESSION   DASUEG010000424 DASUEG010000000
VERSION     DASUEG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331375"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1210
                     /gene="rsgA"
                     /locus_tag="VFX11_07900"
     CDS             173..1210
                     /gene="rsgA"
                     /locus_tag="VFX11_07900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HEX4938580.1"
                     /translation="MSKRKLTRQQNWRIQKIQEEKAARLDRKTKRVETDGEELGLEQE
                     GLVVAHFGTLVEVQVTEAPAADPTAAPAPTVQCHFRANVGAIVAGDRVVLRQGQSHGV
                     IEAVLPRTSLLSRPDPRGQLKPVAANIDFIVLVITPEPPPSSVLIDRYLVAAELQNIP
                     IKILLNKTDLLTDLNEASIDALLNLYRAIGYPVLESSVVTERGLEELQHQLDQHISVF
                     VGQSGVGKSSLVNALLPGVNAKVGGLSETSGLGRHTTTNARLFHFPSGGSLIDSPGIR
                     EFGLWHLEPENLIEGFREFDAWRFDCKFRDCSHQAEPGCALRAAAERGDVHPQRLKNY
                     FAILQSLQETR"
     gene            1249..>2435
                     /locus_tag="VFX11_07905"
     CDS             1249..>2435
                     /locus_tag="VFX11_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX4938581.1"
                     /translation="MDFTYTALTIGAVLVLLSIVLTAVSSRVGFPLLLLFLALGMLAG
                     EDGLGKIPFQNYPASFLIGNLALAIILLDGGMRTRAESFRVALWPALSLATVGVVLTV
                     GITGAFAAWLLDLPLWVGMLLGAIVGSTDAAAVFSLLNNSGVHLQQRVGATLEIESGS
                     NDPMAVFLTVALLEITMQAESGFGMTLLISLVQQFGIGALMGVGAGFLLLRAVNALHL
                     AAGLYALLVCSGGVLIFAATSAVGGSGILAVYLTGILLGNKRLRNKQGILQVLDGAAW
                     LSQIGMFLILGLLVTPSSMMHYTLPALFVALWLTLVARPLAVWVSLIPFVFSVREKFF
                     ISWVGLRGAVPIILALFPMMAGHEHAQLLFNIAFVVVLVSLMTQGFSIPLAASLCRVE
                     VPP"
BASE COUNT          433 a          760 c          737 g          505 t
ORIGIN      
        1 tacatccctg taggctcgac agcggccatc catggccgct gacgttcctg aaccccacgc
       61 cgctctcagg cttccattcc agcaacctat cattctggtc gcacattgcg aaaaccagct
      121 ttaataataa cgtagaatac ccagccgcac cggttttcca cagcgagatc ccatgagcaa
      181 acgcaaactc acccgccaac agaactggcg catccagaaa atccaggagg aaaaagccgc
      241 ccggctggat cgcaagacca agcgcgtgga aaccgatggt gaagaactgg gcctcgaaca
      301 ggaaggtctg gtggtagccc atttcggcac actggtggag gtgcaggtaa ccgaagcccc
      361 tgccgcagac cccaccgccg caccagcgcc gaccgtgcaa tgtcatttcc gcgccaatgt
      421 gggcgccatc gtcgccggcg accgggtggt gttgcgtcag ggccagtccc acggcgtgat
      481 cgaggccgtg ctgccgcgca cctcgctgct gtcgcggccc gatccacgcg gccagctcaa
      541 acccgtagcc gccaacatcg atttcatcgt actggtgatc acgccggaac cgccgccgtc
      601 cagcgtgctg atcgaccgct atctggtggc agcggaattg cagaacattc ccatcaagat
      661 cctgctcaac aaaaccgatc tgctcaccga cctgaacgaa gcgtccatcg acgcgctgct
      721 caacctgtac cgcgccatcg gttacccggt gctggaatcc tcggtggtca ccgagcgcgg
      781 actggaagaa ctgcagcatc agctggacca gcatatttca gtgttcgtgg gccagtccgg
      841 cgtcggcaaa tcgtcgctgg tgaacgccct gctgccgggc gtaaatgcca aggtgggtgg
      901 actgtcggaa acctcgggcc tgggcaggca caccaccacc aacgcgcgcc tgtttcattt
      961 tcccagcggc ggcagcctga tcgattcacc cgggattcgt gaattcggtt tgtggcatct
     1021 ggagccggaa aacctgattg aaggcttccg cgaatttgac gcctggcgct tcgactgcaa
     1081 gtttcgcgat tgcagccacc aggccgaacc gggttgcgct ttgcgcgccg cagcggaacg
     1141 cggcgacgta catccgcaac ggctgaaaaa ttactttgcc atcctgcaat cactgcagga
     1201 aacccgttaa actgaactcc attccctgac cgatggacgc cgtttgccgt ggactttacc
     1261 tacaccgccc tcacaatcgg tgcagtgctg gtgctgctca gcatcgtcct tactgccgtg
     1321 tcttcccgcg tcggcttccc gctgctgctg ctgtttctgg cgctgggcat gctggcgggc
     1381 gaggacggcc tcggcaagat cccgttccag aattatcccg cttccttcct gatcggtaac
     1441 ctggcgctgg ccatcatcct gctcgatggc ggcatgcgca cgcgggcaga atcgttccgt
     1501 gtggccttgt ggccggcgct gtcactggcg acggtaggcg tagtgctcac cgtgggcatc
     1561 accggcgcgt ttgctgcctg gctgctggat ctgccgctgt gggtgggcat gctgctgggg
     1621 gcgatcgttg gctccaccga tgcggcggcg gtattttcac tgctcaataa cagcggagtg
     1681 cacctgcaac agcgggtagg cgcgacgctg gaaatcgaat ccggttcaaa cgatccgatg
     1741 gcggtgtttc tcactgtcgc gctgctggaa atcaccatgc aggctgaaag cgggtttggc
     1801 atgacgctgc tgatcagcct ggtgcagcaa ttcggtatcg gcgcgctcat gggcgtgggc
     1861 gcaggcttcc tgttgctgcg ggcggtgaat gcactgcatc tggcggcggg actgtatgcg
     1921 ctgctggtat gctcgggcgg tgtgctgatt tttgctgcta ccagcgcagt gggtggcagc
     1981 ggcatcctgg cggtgtatct caccggcatc ctgctgggca acaaacgcct gcgcaacaag
     2041 cagggcatcc tgcaggtgct cgacggtgcc gcctggctga gccagatcgg catgttcctg
     2101 atactgggtc tgctggtcac ccccagcagc atgatgcatt acacccttcc cgcgctgttc
     2161 gtggccctgt ggctcacgct ggtcgcacga ccgctggccg tgtgggtcag cctgatcccg
     2221 tttgtatttt cggtgcggga aaaattcttc atctcgtggg tgggattgcg cggcgcggtg
     2281 cccatcattc ttgccctgtt cccgatgatg gcggggcacg aacacgccca gctgctgttc
     2341 aacatcgcgt tcgtggtggt gctggtgtcg ctgatgacac aggggttttc cattccgctg
     2401 gcggccagcc tctgccgggt ggaagtgccg cccga
//
LOCUS       DASUEG010000425         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1682008, whole genome shotgun sequence.
ACCESSION   DASUEG010000425 DASUEG010000000
VERSION     DASUEG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682008"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /locus_tag="VFX11_07910"
     CDS             complement(<1..2179)
                     /locus_tag="VFX11_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4938582.1"
                     /translation="MKKHYLTHFFDPATIAVIGNSERSDSVDVRLFERLRDEFKGKTW
                     LINPRLRGLFANKSFATVDQVPGRVDLAIIVTPDSLIAEQLERCADVGIDSVVVLSHL
                     DDRDGFMRRQFVENLTRVAERRGIRMWGPDCYGFARPPVGIRATLDRGEIRSGKIALI
                     SHSGAICRAVTDWAIANGVGFSTIISLEQAGGVFPGDILDYLVIDQQTECILFYAEGI
                     KDSRSFLSGLRGAARSKPVIVLKSGRHIETPEKVMSHTGALVGNDHVFNAVLERAGIV
                     RADSLTDLFLAARAFPIHRIMRGERIAILSHGNGPAMMACDKAHDLGLPMATLSPEKS
                     AQLASLTSIDSSDNPVFSGGFEVGEEFIKACDIIATDPNVDFLILVITPPKGKVVAEF
                     EEQIAAIQKKSFKPLVVCCMGGDTVEPIKRTLQQKGIPVFSSPDNAVKAAHFLVTYQR
                     NRTFLLQAPASLENTVEPDVEGAQLIIEGVLQEGRKTLNQLESRAVLKAFNIPVNPIQ
                     IARTPNEALVAAEAVGFPVVLKINSPDIVHKSDVAGVTLNVLNAQSVRSAYKKMMEDV
                     QLKCPDARLEGVIVEHMVAAGSNRELLLGIKSDSVFGPVIVFGHGGSLVEILRDIAIS
                     LPPLNSVLASALVDSPKVSRMLKHYHNMPAANRAELENIILRVSEMASELPWIRGLDI
                     NPLVLDDKRGLVLDAVIEVDYLPPAQRRYEHMAIHPYPSYLQAR"
BASE COUNT          433 a          700 c          661 g          421 t
ORIGIN      
        1 agcgcgcctg cagatacgac ggatacggat ggatcgccat gtgctcatag cgacgctgtg
       61 ccggcggcag atagtccact tcgatcacgg catccagcac cagcccgcgc ttgtcatcca
      121 gcaccagcgg gttgatgtcg aggccgcgta tccagggcaa ctcactggcc atctccgaca
      181 cgcgcaggat gatgttttcc agttcggccc ggttggcagc cggcatgttg tgataatgct
      241 tcagcatgcg cgacactttg ggactgtcca ccagcgccga cgccaggacg gaattcagcg
      301 gcggcaggct gatggcgatg tcgcgcagga tttccaccag cgaaccgcca tggccgaaca
      361 cgatcaccgg accgaacacg gaatcggact tgatgcccag cagcaattcg cgattggaac
      421 cggcggccac catgtgctcg acgatcacac cttccagccg cgcatccgga cacttcagct
      481 gcacatcctc catcattttc ttataggcgg accgcaccga ttgcgcattg agcacattga
      541 gcgtaacgcc agcgacatcg gatttgtgca cgatatccgg cgaattgatt ttcagcacta
      601 ccggaaaacc caccgcctcg gccgccacca gcgcctcgtt gggcgtgcgg gcgatctgga
      661 tcgggttcac cggaatattg aacgccttca gcaccgcgcg ggattccagc tggttcagtg
      721 tcttgcgccc ttcctgcagc acgccttcga tgatcagctg cgcgccttcg acatccggct
      781 ccaccgtatt ttccagcgat gccggcgcct gcagcaggaa ggtgcgatta cgctggtagg
      841 tcaccagaaa gtgcgcggcc ttcactgcgt tgtccggcga ggaaaatacc ggaatgccct
      901 tctgctgcag cgtgcgcttg atgggttcca ccgtgtcgcc gcccatgcag cacaccacca
      961 gtggcttgaa gctttttttc tggatcgcgg caatctgctc ttcgaattcc gccaccacct
     1021 tgcccttcgg cggggtaatg accagaatca gaaaatccac gttgggatcg gtggcgatga
     1081 tgtcgcaggc cttgatgaat tcctcgccca cttcgaaacc gccgctgaac accgggttgt
     1141 ccgacgaatc gatcgacgtg aggctggcca gctgggccga tttttccggg ctcagcgtcg
     1201 ccatcggcaa gcccaggtca tgggccttgt cgcaggccat catcgccgga ccgttgccgt
     1261 gggagagaat ggcgatgcgc tcgccgcgca tgatgcggtg gatcggaaac gccctcgccg
     1321 ccaggaacag atccgtgagg gaatccgccc gcacgatgcc ggcccgttcc agcactgcat
     1381 tgaacacgtg atcgttgccc accagcgcgc cggtgtgcga catgactttt tccggtgtct
     1441 cgatatgccg gccgctcttc agcacaatca ccggcttgct gcgggccgcg ccgcgcagac
     1501 ccgacagaaa actgcgggag tccttgatgc cttccgcgta aaacaggatg cattcggtct
     1561 gctggtcgat gaccaggtaa tccagaatgt cgcccgggaa aacaccgccg gcctgctcca
     1621 gggaaatgat ggtggaaaaa cccacgccgt tggcaattgc ccagtcggtg acggcacggc
     1681 aaatggcgcc ggaatgggag atcagggcaa tcttgccgga acggatttcg ccgcggtcca
     1741 gggtggcgcg aatgcccacc ggcggccgcg caaaaccgta gcagtcgggg ccccacatgc
     1801 ggataccgcg ccgctccgcc acccgggtga gattttccac gaactggcgc cgcatgaagc
     1861 cgtcgcggtc atccaggtgg gacagcacca ccacggaatc gatgcccaca tcggcgcaac
     1921 gttccagctg ttcggcgatc agcgaatccg gcgtcacgat gatggccaga tccacccgcc
     1981 ccggcacctg gtcgacggtg gcgaagctct tgttggcaaa caggccccgc aggcgcggat
     2041 tgatcagcca ggtcttgccc ttgaattcgt cccgcaggcg ttcgaacagg cgcacatcga
     2101 cgctgtcgct gcgctcgctg ttgccgatga ccgcgatggt ggcgggatca aaaaaatggg
     2161 tgaggtagtg ctttttcatt atgacgacgt ccgctgggct ggttcaggca gtata
//
LOCUS       DASUEG010000426         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_663705, whole genome shotgun sequence.
ACCESSION   DASUEG010000426 DASUEG010000000
VERSION     DASUEG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663705"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFX11_07915"
     CDS             complement(<1..853)
                     /locus_tag="VFX11_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX4938583.1"
                     /translation="MKPMPDLHHLTPEQLRIFAADLLLQVQSRDQAIALQRNVIAERD
                     DAIKRHKIREEKLTHELALLRRYRYGKGSESLDSQQLSMLEDLFKEDIAAIETELAQI
                     AVPATSEPVKQQPKRQTLPQELPRTPIHHEFAITTCSCGCQLVRIGEDISEKLDYQPG
                     TFSVEQHIRGKWACRQCETVMQAPVPAHVIDKGIPTTGLLAQVLVSKYADHLPLYRQE
                     QIFARAGLHLPRSTLADWVGRCGVELQPLVDALRAELLTHTVLHADETPVAMLAPGKK
                     QTHRAYVW"
     gene            complement(916..1251)
                     /gene="tnpB"
                     /locus_tag="VFX11_07920"
     CDS             complement(916..1251)
                     /gene="tnpB"
                     /locus_tag="VFX11_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX4938584.1"
                     /translation="MLRLDEIWLAREPLDMRAGPDTALARVIHSFGVAKHHTAYVFAN
                     KQANRLKILVVDPFGVWLAARRLHQSKFVWTALREGGAVELDAQQLRALVVGLPWQYA
                     GKNQAISVL"
     gene            complement(1253..1603)
                     /locus_tag="VFX11_07925"
     CDS             complement(1253..1603)
                     /locus_tag="VFX11_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX4938585.1"
                     /translation="MSTSRHDKPKAKRRRHTPDFRLSVLKECEAPGISIAEVARKHQL
                     NANLVHKWRRDLQRSQAPQQDDFVALPVLATPAIQIGTSSNIKVELLSPAGPIVLHWP
                     LSEASQLALWLRQL"
     gene            complement(1697..>2488)
                     /gene="glgP"
                     /locus_tag="VFX11_07930"
     CDS             complement(1697..>2488)
                     /gene="glgP"
                     /locus_tag="VFX11_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX4938586.1"
                     /translation="PTWACQSVAALFDQHMLGWRHEPEILVRADCCISNEGIWLAHSA
                     AKLHLLDHVETLTGIRLDTERPILGFARRMTAYKRPQLLFSDLERLRTISRKLPFQII
                     LAGKAHPHDYSGKDSIAWLHDALRALAPDITGVFLPNYDMTLAQRLVAGVDIWVNTPL
                     PPLEASGTSGMKAAINGVPSLSVLDGWWAEGCIEGVTGWAIGDGRADTADAESLYNKL
                     EQEILPLYYNDRATWATLMRAVISRNGSMFNSHRMVRRYAAETYL"
BASE COUNT          559 a          696 c          682 g          551 t
ORIGIN      
        1 cccacacgta agcacggtgg gtttgcttct tgccgggtgc cagcatggcc accggtgttt
       61 catcggcgtg caacacggtg tgggtcagca gttcggcgcg aagggcgtct accagcggtt
      121 gcagctccac gccgcagcgg ccaacccagt ccgccaaggt cgagcgtggc agatgcagac
      181 cggcacgggc aaagatctgt tcctgccggt acagcggcaa gtgatcggcg tatttcgata
      241 ccagcacctg cgccagcaat cccgtggtcg gaatgccttt gtcgatcaca tgggcaggca
      301 ccggcgcttg catcaccgtc tcgcattgac gacaggccca tttgccccgg atatgctgct
      361 cgacactaaa ggtgccgggc tggtaatcca gcttctcgct gatgtcttca ccgatgcgaa
      421 ccaactggca gccgcaggaa caggtggtga ttgcaaactc atgatgaatc ggggtgcgcg
      481 gaagttcttg aggtaatgtc tgacgtttgg gctgctgttt gaccggttct gatgtggctg
      541 gaactgcaat ctgcgccagc tcggtttcaa tcgccgcgat gtcttccttg aacagatctt
      601 cgagcatgct cagctgctgg ctgtccagac tctcgctgcc tttgccgtag cgataacgcc
      661 gcagcaatgc cagttcgtgt gtcagcttct cttcgcggat cttgtgccgc ttgatggcgt
      721 catcacgttc ggcgatgacg ttgcgctgta atgcaattgc ctgatcgcgg gactgcacct
      781 gcaacagcaa atccgctgcg aagatacgca gctgttccgg tgtcagatga tgaagatcgg
      841 gcatgggttt catgtccgca ctgtgccaga aaccggcacg ccgttcgacg ttttttgtgt
      901 atgaaaaaat gacggctaca acaccgatat tgcctggttt ttgccagcat attgccaggg
      961 caggcccacg accagggcac gtaactgttg cgcgtccaat tcgaccgcac cgccttcacg
     1021 caaggccgtc catacaaatt tactctgatg cagtcgccgg gccgccagcc agacgccaaa
     1081 cggatcgacc accagaatct tcaggcgatt ggcctgcttg ttggcaaaca catacgcggt
     1141 atgatgcttg gcgacaccga aggaatgaat gacacgcgcc agggcagtgt caggcccggc
     1201 ccgcatatcc agcggctccc gcgctagcca gatctcatca agacgcagca tgctataact
     1261 gcctgagcca gagggccagc tgactcgctt cggataacgg ccagtgcagc acaatgggac
     1321 ccgccggaga aagcagctcc actttgatat tggaactggt accgatctga atggcaggcg
     1381 tggccagaac cggcaatgcg acaaaatcat cttgttgcgg cgcctggctg cgctgaagat
     1441 cacggcgcca tttatgcacc agattggcat tgagttgatg tttgcgagcc acttcggcga
     1501 tggagatgcc cggtgcttcg cactccttca gcacgcttag cctgaagtcc ggggtatggc
     1561 gtcgacgctt ggctttgggt ttatcgtgtc ttgatgtact cataataagt gtccacttaa
     1621 aaataggtgg acactacagt agctggttcg cagcagctga aaagatggga tttaccgggc
     1681 ggttacaata cgctcactag agatatgttt cggccgcata acgccgtacc atgcgatgac
     1741 tattgaacat agatccatta cgagaaataa ccgctcgcat aagcgtggcc cacgttgcac
     1801 gatcattgta gtaaagcggc aatatctctt gctccagctt attgtagagg gactctgcat
     1861 cggcagtatc tgctcggccg tcgccaatcg cccagcccgt aacgccttca atgcagccct
     1921 ccgcccacca gccatctagc accgagagac ttggaacgcc gttaatggcc gccttcattc
     1981 cgctggtgcc ggaagcctcc aatggcggca gtggagtatt tacccatata tccaccccgg
     2041 ccaccaaacg ctgtgcaagg gtcatgtcgt aattaggtag aaaaacgccg gtaatatctg
     2101 gcgctaacgc acgaagtgca tcatgtaacc aggcgatact gtctttaccg gaataatcgt
     2161 ggggatgcgc cttacctgcc aaaatgatct ggaacggcag tttccgcgaa atggttcgca
     2221 aacgttccaa atccgaaaag agtaattggg gtcgtttata agctgtcata cggcgggcaa
     2281 accccagaat cgggcgctcc gtatccaacc gtatacccgt cagcgtttct acatggtcga
     2341 gcaaatgcaa cttggcagcg ctatgtgcca gccagatgcc ttcattgctg atgcaacaat
     2401 cggcacgaac taaaatctca ggttcatggc gccatccaag catgtgttga tcaaataatg
     2461 cagcaactga ttggcatgcc caagtggg
//
LOCUS       DASUEG010000427         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1060679, whole genome shotgun sequence.
ACCESSION   DASUEG010000427 DASUEG010000000
VERSION     DASUEG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060679"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFX11_07935"
     CDS             complement(<1..973)
                     /locus_tag="VFX11_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX4938587.1"
                     /translation="MSLSRRTFLGALAASMTAASCASLPRLSIEKSSGSDHDYILTAE
                     PTPIQLVSAGPTPAWCFGGSYPAPVLRARQGQKLRVLFRNRLPEATTIHWHGIRLDIA
                     MDGVPYLTQPPIEPGAEFLYEFTPPDAGTFWYHPHMNSVEQLGKGLVGILIVEEAQPV
                     AFDLDLPLALKTWHINEDGSFSPFSIPRNAARMGTPGRWETVNGQNKPVLEIPAGGLV
                     RLRFANVDNTVIYKLAVKNHPAWVIAVDGNPVAKPYPLVAHEIGAGMRLDVALIAPKK
                     AGEVIQVQNGKGKLFFDFVTLKTVGEPVRGERTIPVLPLNPIPQPDLA"
     gene            complement(1095..1565)
                     /locus_tag="VFX11_07940"
     CDS             complement(1095..1565)
                     /locus_tag="VFX11_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938588.1"
                     /translation="MTVKIKASIKRAKQVNTDVDALYSVLTRPAEAGQFFPRVSKITA
                     LGDERFHWTLEPMGTQKFQHQVEYASQWEYDKAQKTVRWRALPDQGNARISGVWQLEP
                     RPASVNLALEIRAELLLDLPRWMQIVAEPIVQTEFQKLLNNYFDNVVAFCQPPA"
     gene            1857..>2421
                     /locus_tag="VFX11_07945"
     CDS             1857..>2421
                     /locus_tag="VFX11_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938589.1"
                     /translation="MILDSMLMGMPLPARRRVEAALDNISVVLQMRNPRVFASMAPSA
                     LRGLVMKEGKRKDVIAMKSAHPVTFDMTYKMDFPEMNKLYRRAVASQWNADTALDWSI
                     DVDPLNPEVPILSNDFIPDSVFDELPVKFSVLQRRELNYNLAAWMLSQFMHGEQGALY
                     ASAQVTESVQWMEGKFFGATQVMDEARH"
BASE COUNT          501 a          724 c          703 g          493 t
ORIGIN      
        1 tggccagatc cggctgtgga atcggattca gtggcaacac gggaatcgtg cgttcacccc
       61 gtaccggttc gcccacggtt ttcagcgtga caaaatcaaa gaacagtttg cctttgccgt
      121 tctgcacctg aatcacctca ccggcttttt tcggagcaat caacgccaca tccagccgca
      181 tgccggcacc gatttcgtgg gcgaccaaag ggtagggttt cgccaccgga ttgccatcca
      241 ccgcaatcac ccaggccggg tgattcttca ccgccagctt gtagatcacg gtgttgtcca
      301 cgttggcaaa acgcagccgg accaatccgc cggcaggaat ctccagtacc ggtttgttct
      361 ggccgttcac ggtttcccaa cggccgggcg tacccatgcg cgcggcgttg cgcggaatgc
      421 tgaacgggga aaaactgcca tcttcattga tgtgccaggt tttcagcgcc agcggcagat
      481 ccagatcgaa tgccaccggt tgcgcttcct ccacgatcag tatgcccacc agccccttgc
      541 ccagctgttc cacgctgttc atgtgcgggt gataccagaa ggtaccggcg tcaggcgggg
      601 tgaattcata cagaaattcc gcgcccggct cgatgggggg ctgggtcagg taaggcacac
      661 cgtccatggc gatatcgaga cggataccgt gccagtgaat ggtggtggct tccggtagcc
      721 ggttgcgaaa cagcacccgc agtttctggc cctgacgtgc ccgcagcact ggcgctggat
      781 agctgccgcc aaaacaccag gccggcgtcg gacctgcgct caccagctgg attggcgtgg
      841 gctcggccgt cagtatgtag tcatgatcgg aaccgctgga tttttcaatg gatagacggg
      901 gcagggaagc gcaggacgct gctgtcatcg aggcagccag cgctcccaga aacgtgcggc
      961 gggataagct catgcaaggt caccggtaaa gctggaaacg tgcagtatgc cagaagtgaa
     1021 aatcgacgaa tcaaaaaaaa tgcccgcttg gcgggcaatc atttggcaat tgccaaacac
     1081 tcttcaaagg tgggttacgc ggggggctgg cagaaagcca ccacgttgtc gaaataattg
     1141 ttcagcagtt tctggaattc agtctgcacg atgggttccg ccacgatctg catccagcgc
     1201 ggcagatcca gcagcagttc ggcgcggatt tccagcgcca gattcacgct ggcaggccgt
     1261 ggttccagtt gccacacccc ggaaatgcgg gcattgccct gatccggcag agcgcgccag
     1321 cgcaccgtct tctgcgcttt gtcatattcc cactgcgacg catattccac ctgatgctgg
     1381 aacttctggg tgcccatcgg ctccagcgtc cagtgaaaac gctcgtcgcc cagggctgtg
     1441 atcttgctca cccgcgggaa gaactggccg gcttcagcgg gcctggtcag cacggaatac
     1501 agtgcatcga cgtcggtgtt cacctgcttg gcgcgcttga tcgacgcctt gatcttcacc
     1561 gtcatcggcg gttctccaga aatgagatca caccattcta gtgattgccg cccttgaaac
     1621 attggcgccc cggccagccg gtgcaggcaa agctgcaagc cgggtggcga ccagagtggc
     1681 ccccgctttg tcaggaaaaa tgacatgaag ggcgatctgc gtcagacaat gccggatttt
     1741 ggcggccaat ccccttgtga actggccgct ttagactaca ttgtcaaatt gtaggacaat
     1801 tttgttcaaa tccagctgca tcatctgccg actccactac aagaaagagg ctcaccatga
     1861 ttcttgattc catgctgatg ggcatgccct tgcccgcccg ccgccgtgtc gaagccgctc
     1921 tcgacaacat ttccgtggtg ctgcagatgc gcaatccccg ggtgtttgcc tccatggcgc
     1981 cctcggccct gcgcggtctg gtgatgaagg aaggcaagcg caaggatgtg attgcgatga
     2041 aatcagccca tccggtgacc ttcgacatga cctacaagat ggattttccg gagatgaaca
     2101 aactctaccg gcgggccgtg gcgagccagt ggaacgccga taccgcgctg gactggtcca
     2161 tcgacgtgga ccccctcaat cccgaggtgc cgatcctttc caatgacttc atccccgatt
     2221 ccgtattcga cgaactgccg gtgaagttca gcgtgctgca gcggcgcgaa ctcaactaca
     2281 acctggcggc ctggatgctg agccagttca tgcacggcga gcagggcgcg ctgtatgcct
     2341 cggcccaggt caccgagagc gtgcagtgga tggagggcaa atttttcggc gccacccagg
     2401 tgatggacga ggcccgccac c
//
LOCUS       DASUEG010000428         9866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1749379, whole genome shotgun sequence.
ACCESSION   DASUEG010000428 DASUEG010000000
VERSION     DASUEG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749379"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..396)
                     /locus_tag="VFX11_07950"
     tRNA            complement(311..396)
                     /locus_tag="VFX11_07950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Leu,seq:caa)
     gene            534..1568
                     /gene="queA"
                     /locus_tag="VFX11_07955"
     CDS             534..1568
                     /gene="queA"
                     /locus_tag="VFX11_07955"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX4938590.1"
                     /translation="MKRTDFHYDLPDHLIARYPAEQRSGSRLLVLGKQDGSLADRQFR
                     DLPGYLQPGDLLVFNNTRVIPARLFGQKDTGGKVEILVERITGEQTCLAHVRASKSPK
                     PGSRILIDGGAELRMEGRAGDLFQLKHESDDSLAKGTLLDLLEQHGHMPLPPYIDRAD
                     ENLDKSRYQTVYAQTPGAVAAPTAGLHFDDAMLEQLRASGIETAFVTLHVGAGTFQPV
                     RADDIREHQMHAEWLEVSAQTVARINAVKQRGGRVIAVGTTSVRCLETAAQAGNLQPY
                     QGDTRIFIYPGYEFRVIDGLITNFHLPESTLIMLVSALAGRDNVLNAYRHAVEQQYRF
                     FSYGDAMLVL"
     gene            1664..2779
                     /gene="tgt"
                     /locus_tag="VFX11_07960"
     CDS             1664..2779
                     /gene="tgt"
                     /locus_tag="VFX11_07960"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX4938591.1"
                     /translation="MKFDLLGTDSGARRGRLTFDRGAVETPAFMPVGTYGSVKAMLPR
                     DIDAIGAHIILGNTFHLMLRPGTEIIKLHGDLHNFMQWQGPILTDSGGFQVFSLGKMR
                     KITEEGVRFQSPVDGSPVFLSPEISMQVQRDLGADIVMIFDECTPYPATEAEARKSME
                     LSLRWAKRSKEGHGDNPSALFGIVQGGMYEELRLESLAGLTQIGFDGYAIGGLSVGEP
                     KEEMLKVLNYLSPHLPQDRPRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHLF
                     TSTGVIKIRNAAHKTDTGPLEQDCDCYTCQHFSRAYLHHLDRCKEILSSQLNTIHNLR
                     YYQRLMAGLRAAIETGTFDRFVTDFYLKRGETVPPLT"
     gene            2853..3191
                     /gene="yajC"
                     /locus_tag="VFX11_07965"
     CDS             2853..3191
                     /gene="yajC"
                     /locus_tag="VFX11_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX4938592.1"
                     /translation="MSFFIADALADSSAAAATPGGSPLSFWGMMIFMVVIFYFMIIRP
                     QSKRNKEHKNLLASLAKGDEVVITGGMLGKVTKVTDDFIVAEVAPNVEISFQKGAITA
                     VLPKGTLKSI"
     gene            3267..5153
                     /gene="secD"
                     /locus_tag="VFX11_07970"
     CDS             3267..5153
                     /gene="secD"
                     /locus_tag="VFX11_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX4938593.1"
                     /translation="MLNKHPLWKDLLVVFLAMFGFIYALPNLYPDDPALQITGAKPGV
                     ETRQEILDQAVTALGQGGIAIKSSELEEKSGLIRFANVDDQLKAHSLVKKSLDAQHPD
                     EYVAALNLAPTTPGWLQSLGAGPMKLGLDLRGGVHFLMEVDMDKALDNRVSNSINSMR
                     DRFRDDRIRAKTLRQVQGQPAIEMVFSDVGMLDKARAILRKDFREFQVAERQEGTDYR
                     LTLSLNEQSLKDIQEYALDQNLTSIRNRVNELGVSEPVVTRQGANRIVIQLPGIQDTA
                     AAKRIIGRAANLEFRMVDAKDSGISTARAPAGTEFVPVRNSPRQVLIKKENIVTGENV
                     IDAKPNYDQNGLPMVSVTLDTAGGRRMMKNTTAHLKESMAVLFIESKPETRQIIVDGK
                     PVEKTVTRETREAISVATIQGIFGSQFQITGLDSFEEAQELALLMRAGALAAPMYFVE
                     ERTVGPSLGAQNIEAGVESLLLGFALVVVFMIAVYKVCGVIANIALFMNLLLLVACMS
                     LIPGAVLTLPGMAGIVLTLGMAVDANVLIFERIREEIRNGMTPHAAIHAGYDRAWLTI
                     MDSNITTLLVGLVLFSLGSGPVKGFAVTLSIGILTSMFTAVTGSRAIINMIYGGRALQ
                     KLSI"
     gene            5164..6081
                     /gene="secF"
                     /locus_tag="VFX11_07975"
     CDS             5164..6081
                     /gene="secF"
                     /locus_tag="VFX11_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX4938594.1"
                     /translation="MAIKTKFDFMKHRHLMSGVSLVAVILSLVSLAINFMELGLDFTG
                     GTQVEVEYSQPADLDQVRQTLAGSHYADAIAQHFGKPEEVLVRVPPKEGEDKDVVGHA
                     VHDLLKQVNPDAKLKRIEFVGPQVGGELRDKSGIALLVSFAIILIYVSIRFRFKFAVG
                     GVTALVHDVIVTLGFFSATRLVFDLTSLAAVLTIIGYSINDTIVVYDRIRENVRKLRN
                     ETMSDIINISLNETLSRTIMTASTVFLVLVALALLGGEMIFSFAMALIVGVIVGTYSS
                     VYVASNMLLIQNLTREDFMEQEKVVDDMP"
     gene            complement(6173..6328)
                     /locus_tag="VFX11_07980"
     CDS             complement(6173..6328)
                     /locus_tag="VFX11_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938595.1"
                     /translation="MNWQDLKASLLAFFQGLSLPNQYHQEPELKPVPIENNSARNRSD
                     RRPADRR"
     gene            complement(6476..7279)
                     /locus_tag="VFX11_07985"
     CDS             complement(6476..7279)
                     /locus_tag="VFX11_07985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4938596.1"
                     /translation="MHPLLNTALSVARSTGDMISKAFEQVDLVDVEAKGANDFVTKVD
                     RASEERIIDGLRKRYPDHSFFGEENGLVEGQGEGKDYVWVIDPLDGTTNFIHGVPHFA
                     ISIGLKIRGQLEVAVVLDPMRKEEFTAARGRGAQLNGRRIRVTSRTKLDGALLATGFP
                     FRPDQNRVFDAYMGMFGDFARSTAGIRRAGAAALDLAYVASGRYDGFWEFGLSEWDMA
                     AGTLLVTEAGGLIGDIKGGLDHLKTGDVVCAPPKLFKAMLQTIHPHLPK"
     gene            7425..8180
                     /locus_tag="VFX11_07990"
     CDS             7425..8180
                     /locus_tag="VFX11_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4938597.1"
                     /translation="MQLLDRLRIVMINTSDSGNIGAAARAMKTMGLRQLVLVQPEEVP
                     TAKATARASAAADLLHKARVVQTLDEAIGDCQLVFGTSARLRTIPWPLIDPRQGAARV
                     MNEPVGADIAILFGREDAGLTNEELRRCHFHICIPANEEYPVLNIGAAIQVICYEMRM
                     AALERQANPVVPDLSGMQQWDEPLVSSEDMERFLRHFEETLLDIDFFDPNNPKQLLTR
                     VRRFFLRTRMDRLEMNLLRGVLSTVQKRVKERS"
     gene            8478..9119
                     /locus_tag="VFX11_07995"
     CDS             8478..9119
                     /locus_tag="VFX11_07995"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4938598.1"
                     /translation="MTSQRTRDRLIGRLQDQGIASFEVLDAIRSIPRHLFVDEALAHR
                     AYEDTALPIGHNQTISQPYIVARMTELLIQGLPQGKRLKNVLEIGTGSGYQTAILSRF
                     AEQVCSLERIKSLQDKARKRLQALKIRNVLLRYADGSVGWKDKGPFDGILAAAAPAEI
                     PQELLDQLAVGGRLLIPVGESKQVLYRIIRTETGFERQKMEDVKFVPFRSGVR"
     gene            9379..>9866
                     /locus_tag="VFX11_08000"
     CDS             9379..>9866
                     /locus_tag="VFX11_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4938599.1"
                     /translation="MARNQTVHFLIVLCLGVSLSACETSRYQPTRNVYRSADHAAQGF
                     HTVRPGETLYSIASRNRRNPDELARANGIKPPYQIYPGQIIYFNKTASAPVSRSKPAA
                     KPPATNQTSRYKKPASNTKPIQEKVANQRDKKVDISWKWPAGGPVIETFSTAGQVNKG
                     IDL"
BASE COUNT         2087 a         2954 c         2860 g         1965 t
ORIGIN      
        1 aaaacgccgc gatctggatg taccaccgaa caaaggccag tgtcaggcga tgcggtccaa
       61 gaccgtcagc cgcatggatg cggctgtcga gcccccaggg cagggttcac gggggggcgc
      121 ccagctcgtg tcttgaactg catcacctga cactggccgt gctacgaagt cccaagtgcg
      181 attcaatttc aaagcgcaaa gcaccccgca gcttttcagc cgcaaatacg cagcacagtg
      241 cggcaaaggc agagcgattt atcaaactca ggtttagata gcagaaagca catcaaaaca
      301 ggaaggaaaa tggtgccgct ggtcagactc gaactgacat gagcagagct cggttgattt
      361 tgaatcaact gcgtctaccg gtttcgccac agcggcattg aggggcaaaa cgagaggggc
      421 gattataaca gcgcgaatcg gccggtcaac caagcgtttc ggacaagccc ccaaaccgct
      481 ataattgccg cccttttgcc caggcgctgc ctgtctcacc cagccagaga gtcatgaaac
      541 gcaccgactt ccactacgac ctgcccgacc acctgatcgc ccgctacccg gccgaacagc
      601 gcagcggcag ccgcctgctg gtgctgggca aacaggacgg cagcctcgcc gaccgccagt
      661 tccgcgacct gccgggctac ctgcaacccg gcgatctgct cgtcttcaac aacaccagag
      721 tcatccccgc ccgcctgttc gggcagaaag acaccggtgg caaagtggaa atcctggtgg
      781 aacgcatcac cggcgagcag acctgcctgg cccacgtccg cgccagcaaa tcccccaagc
      841 ccggcagccg tatcctcatt gatggcggtg cggagctgcg catggagggc cgcgccggcg
      901 acctgttcca gctcaaacat gaaagcgatg atagccttgc gaaaggcacc ctgctggacc
      961 tgctcgaaca gcacggccac atgcccctgc caccctacat cgaccgcgcc gacgaaaatc
     1021 tcgataaaag ccgctaccag accgtctacg cccagacccc cggcgctgtc gccgcgccca
     1081 ccgccggtct gcattttgat gacgccatgc tggaacaatt acgcgccagc ggaatcgaaa
     1141 ccgcgttcgt gacgctgcat gtgggggccg gcaccttcca gccggtgcgc gccgacgaca
     1201 tccgcgagca ccagatgcat gctgaatggc tggaggtttc cgcgcagacc gtcgcccgga
     1261 tcaacgcggt caaacaaaga ggtggccgcg ttatcgcggt gggtacaacc tccgtccgtt
     1321 gcctggaaac cgcggcgcaa gctggcaatt tgcagcccta ccagggtgac acccggatct
     1381 tcatctaccc gggctacgag ttccgcgtca tcgacggcct gatcaccaat ttccacctgc
     1441 cggagtccac cctcatcatg ctggtgagcg cactggccgg ccgcgacaac gtgctaaacg
     1501 cctaccgcca cgcggtggag cagcaatacc gttttttcag ctatggcgac gccatgctcg
     1561 tgctgtaaag gttccgctct gcctgacgcg ggcgctaagt gaaacagata actgaataga
     1621 acattgaatc gaccgacaga aacgcaggat tcgggacaac agaatgaaat ttgatttgct
     1681 gggaacagac agcggcgccc gccggggccg cctcaccttc gatcgcggtg cggtggaaac
     1741 gcctgccttt atgccggtag gcacttatgg ttcggtgaaa gccatgctgc cgcgcgacat
     1801 cgacgcgatc ggcgctcaca tcattctcgg caacaccttc cacctgatgc tgcggcccgg
     1861 caccgagatc atcaaactgc acggcgatct ccacaatttc atgcagtggc agggtcctat
     1921 tctcaccgac tccggcggtt tccaggtgtt ctccctgggc aagatgcgca agatcacgga
     1981 agagggcgtg cgcttccagt ccccggtgga cggcagcccc gtcttcctca gcccggaaat
     2041 ctccatgcag gtgcagcgtg acctgggcgc cgacatcgtg atgatcttcg acgaatgcac
     2101 cccgtacccg gccaccgagg ccgaggcgcg caaatccatg gaactgtccc tgcgctgggc
     2161 gaaacgctca aaagaaggcc acggcgacaa tccgtcggcg ctgttcggca tcgtccaggg
     2221 cggcatgtat gaagaactgc ggctggaatc cctggcgggc ctgacacaga tcggtttcga
     2281 cggctatgcc atcggcggcc tgtcggtggg cgagccgaaa gaggaaatgc tcaaggtgct
     2341 gaattacctg tcgccgcacc tgccccagga ccgtccccgc tacctgatgg gggtgggcaa
     2401 gccggaggat atcgtggagg ccgtgcgccg gggggtcgat atgttcgact gtgtcatgcc
     2461 cacccgcaac gcccgcaacg gccacctgtt caccagtacc ggcgtgatca agatccgcaa
     2521 tgccgcccac aagaccgaca caggccccct ggagcaagac tgcgactgct atacgtgcca
     2581 gcacttctcc cgcgcctatc tgcaccacct ggaccgctgc aaggaaatcc tcagttccca
     2641 gctcaacacc atccacaatc tgcgctatta ccagcgcctg atggccggtt tgcgcgctgc
     2701 tatcgaaacg ggtacatttg accgctttgt gacggacttc tacctgaagc ggggcgaaac
     2761 cgtgccgccg ctgacctgaa attcctgctc catgcccgaa ttttccaact tttgacccac
     2821 tatccaattg ttgtcattca actgaggtac atatgagctt tttcatcgca gatgcactgg
     2881 ccgactcttc cgctgccgcc gcaactcccg gcggtagtcc gctgagcttt tggggcatga
     2941 tgatcttcat ggtcgtgatc ttctatttca tgatcattcg cccccagtcc aagcgcaaca
     3001 aggaacacaa gaacctcctc gccagtctgg ccaagggaga cgaagtggtg atcaccggtg
     3061 gcatgctggg caaggtcacc aaggtgacgg acgatttcat cgtggcggaa gtggctccca
     3121 acgttgaaat ttccttccag aaaggggcca tcacggccgt gctgccaaaa ggcacgctga
     3181 aaagcatctg ataccgctgg ttgtcgcggg gcgtgcgttg cgctccgccg gtcaggatcg
     3241 acgtcgaaaa cattagggct caaaccatgc tgaacaagca tcctttatgg aaagacctgt
     3301 tggtggtgtt cctggccatg ttcgggttca tctatgcgct tcccaacctc tacccggacg
     3361 acccggccct gcagatcacc ggcgccaagc ccggcgtgga aacccgccag gaaatcctcg
     3421 accaggccgt tactgcgctg gggcaggggg gcattgccat caagtcctcg gagctggagg
     3481 aaaagtccgg cctgatccgc ttcgccaatg tggatgacca gctcaaggcc cacagcctgg
     3541 taaaaaaatc ccttgatgcg cagcatcccg atgagtatgt ggcagcgctc aatctggctc
     3601 ccaccacgcc tggctggctg cagtccctgg gcgccggtcc catgaagctg ggcctggacc
     3661 tgcggggtgg cgtgcacttc ctgatggaag tggatatgga caaggcgctg gacaaccggg
     3721 tcagcaactc catcaacagt atgcgcgacc gcttccgcga cgaccgcatc cgtgccaaga
     3781 cattgcgtca ggtgcagggc cagcctgcca tcgaaatggt attcagtgat gtcggcatgc
     3841 tcgacaaggc gcgcgccatc ctgcgcaagg attttcgcga gtttcaggtg gcggaacgcc
     3901 aggaaggtac cgactaccgg ctgacattga gcctgaatga gcagtcgctg aaagatatcc
     3961 aggaatacgc gctggatcag aacctgacgt ccatccgcaa ccgggtcaac gagctgggtg
     4021 tgtcggagcc ggtggtaacc cggcaggggg ccaaccgtat cgtgatccag ctgccaggca
     4081 tccaggacac tgcggcggcc aaacggatca tcggccgcgc cgccaacctg gaattccgca
     4141 tggtggacgc gaaagacagt ggcatttcca ccgcgcgggc tccggcgggt actgaatttg
     4201 taccggtgcg caattccccg cgtcaggtgc tgatcaagaa ggaaaatatc gtcaccggcg
     4261 aaaacgtcat cgacgccaag ccgaactacg atcagaacgg cctgcccatg gtgtctgtca
     4321 cgctggatac ggcgggcggt cgccgcatga tgaaaaacac cacggcacac ctcaaagagt
     4381 ccatggcagt gctgttcatc gaatccaaac cggaaacccg gcagatcatc gtggacggca
     4441 agccggtgga aaagacagtc acccgcgaaa cccgcgaggc gatcagcgtg gccaccattc
     4501 agggcatttt cggttcccag ttccagatca ctggactgga cagcttcgag gaagcccagg
     4561 aactggcgtt gctgatgcgg gccggcgcgc tggcggcacc gatgtatttt gttgaagagc
     4621 gcacggtggg tccaagcctg ggtgcacaga acatcgaagc cggtgtcgag tcgctgctgc
     4681 tgggattcgc actggtggtg gtgttcatga tcgcggtgta caaggtgtgt ggcgtgatcg
     4741 ccaacatcgc cttgttcatg aacctgctgc tgctggtcgc ctgcatgtcg ctgataccgg
     4801 gcgcggtgct cactttgccc ggcatggccg gtatcgtgct gaccctgggt atggccgtgg
     4861 acgcgaacgt cctgatcttc gagcgtatcc gcgaggaaat ccgcaacggc atgacgccgc
     4921 acgccgcgat tcatgctggt tacgaccgtg cctggctcac catcatggat tccaacatca
     4981 ccacgctgct ggtgggtctg gtgctcttct cgctgggttc cggcccggtg aaaggctttg
     5041 ccgtcaccct gtcgatcggc atcctgacct ccatgttcac cgccgttacc ggcagccgcg
     5101 cgatcatcaa catgatctat ggcggacgcg cgctgcaaaa actgagtatc tgaggaatcc
     5161 gtcatggcga ttaagactaa atttgatttc atgaaacacc gccatttgat gagtggcgtt
     5221 tcgctggtag cagtcatcct gtcgctggtg tccctggcaa tcaatttcat ggagctgggt
     5281 ctggatttca ccggcggtac tcaggtggaa gtggagtatt cgcagccggc ggatctggat
     5341 caggtgcggc agacgctcgc cggcagccac tatgctgacg ccattgccca gcatttcggc
     5401 aaacccgaag aagtactggt gcgggtgcct ccaaaagaag gcgaggacaa ggatgtggtg
     5461 ggtcatgcag tgcatgatct gctgaagcag gtcaatccgg atgccaagct caagcgcatc
     5521 gaattcgttg ggccgcaggt gggaggcgaa ctgcgggaca agagcggcat cgcgttgctg
     5581 gtatcgttcg cgattattct gatctacgtg tcgattcgct tccgtttcaa atttgcggta
     5641 ggcggcgtga ctgcgctggt gcacgacgtg atcgtgaccc tgggcttttt ctcggcgacg
     5701 cgactggtgt ttgatctgac ctcgctggct gcggtgctca ccatcatcgg ttattccatc
     5761 aacgacacca tcgtcgtgta cgaccggatt cgtgagaacg tgcgcaagct gcggaatgaa
     5821 accatgtctg acattatcaa catctcactg aacgaaaccc tgagccgtac catcatgacc
     5881 gcttcaacgg tattcctggt actggtagcg ctggcgctgt tgggcggtga gatgattttc
     5941 tcgttcgcaa tggcactgat tgtcggcgtg atcgtgggaa cctattcgtc tgtgtatgtt
     6001 gccagcaaca tgctgctgat ccagaacctc acccgtgagg atttcatgga gcaggaaaag
     6061 gtggttgacg acatgccttg agcgggctgc aacgtatcaa acccggccgg tgcaacgcac
     6121 ggccgggttt ttttttggat cgaagtcagg ggctggatcg gattgggtgc ctttagcggc
     6181 ggtcggcagg gcgccggtcg gagcggtttc gggcagaatt gttctcaatg ggcaccggct
     6241 tcagttccgg ctcctggtgg tactggttgg gcagactcaa gccttggaag aacgccagca
     6301 acgaagcctt caaatcttgc cagttcatac ggaagccctc tcggcactgg ggataaaacc
     6361 atcccgacag ttctgagtat agaccagctc gcccgtattc cgtccacgtc aggctgattg
     6421 aatcttttgc ctgccgcgat agcggcaggc aatgggcggg tggggctgcg ccagattatt
     6481 tgggcaggtg ggggtggatg gtctgcagca tggccttgaa tagcttgggg ggggcgcaaa
     6541 ccacatcgcc ggttttcagg tggtcaagcc cgcctttgat gtcaccgatc aggccgccgg
     6601 cctcggtcac cagcagagta cctgccgcca tgtcccactc gctcaggcca aattcccaga
     6661 aaccgtcgta gcggccggaa gccacatagg ccagatccag tgccgctgcc ccggcgcggc
     6721 gaatgccggc ggttgagcgg gcaaaatcgc caaacatgcc catataggcg tcgaaaacgc
     6781 gattctggtc cgggcggaag gggaagccgg tggcgagcag ggcgccatcg agcttggtgc
     6841 ggctggtgac ccggatgcgg cggccattca attgggcgcc ccggccccgg gcggcggtaa
     6901 attcttcctt gcgcatcggg tccagcacca cggccacttc cagctggccc ctgattttca
     6961 ggccgatgga aatcgcaaaa tggggcacgc cgtggataaa gttggtggtg ccgtccagcg
     7021 ggtcgatcac ccagacgtaa tccttgcctt cgccctggcc ttccacaagg ccattttctt
     7081 caccgaagaa gctgtgatcc gggtagcgct tgcgcaggcc gtcgatgatg cgctcttccg
     7141 aggcgcggtc caccttggtg acgaaatcgt tggcaccctt ggcttccaca tcgaccagat
     7201 ccacttgctc aaaagccttg gaaatcatgt cgccggtgga gcgggcaacg ctcagggcag
     7261 tattcagcag tggatgcatg cgatgggttc ctcttaaaga gcgggcgatt cagggggccg
     7321 aatgatacca gaattgcccg ccctttaaga ggaacccgcg gcccgcttca gcaaaatctg
     7381 gtaccatttt ggcccttttc agtctttgcg agcgcgtatt tccgatgcaa ctgctcgacc
     7441 gtctgcggat cgtgatgatc aataccagtg actccggcaa catcggtgcc gccgcccggg
     7501 ccatgaaaac catgggcttg cgccagctgg tgctggtaca gccggaggag gtccccaccg
     7561 cgaaggccac cgcccgggcg tctgcggcgg cagacctgct gcacaaggcg agagtggtgc
     7621 aaacgctgga cgaggccatc ggcgactgcc agctggtgtt tggcaccagt gcccgcctgc
     7681 gcaccatccc ctggcccctg attgatcccc gccaaggcgc ggctcgtgtc atgaatgagc
     7741 ctgtaggtgc cgatattgcc attctctttg gccgggaaga cgctggctta accaacgaag
     7801 aactgcgacg ctgtcacttc cacatctgca ttccggcgaa tgaggaatac ccggttctca
     7861 atatcggcgc cgccatccag gtcatctgct atgaaatgcg gatggccgcg ctggaacggc
     7921 aggcaaatcc ggtggtgccg gacctgtcgg gtatgcagca atgggacgag ccgttggtgt
     7981 cgtcagagga tatggagcgg ttcctcagac attttgagga aaccctgctt gatatcgact
     8041 tcttcgatcc caataacccg aaacagctgc tgacccgggt gcggcgtttt tttctgcgca
     8101 cacggatgga ccggctggag atgaatctgc tgcgcggtgt gctgtcgacg gtacagaagc
     8161 gggtaaaaga gcggtcctga gccgcgccac agaattgaaa ggtcggagtg aagccgttcc
     8221 agcgaccggg accgtcagca gcagggatgc tgctgtcgag cgtacaggga tgtattcacc
     8281 gcgtgtcctg ccaggccgtc cctcttctac ttcgactctt tcgcaattcc cgctcgccgt
     8341 ttcatttttc atcttttaca atgaatcgct tgaataataa tgaaaattgg caatggcaca
     8401 gtctggatac caaccggaac ccatccccga ccgcgaggaa gcactgagtg aatgatcttc
     8461 acgtcaacgg aatcggcatg acctcgcagc gaacgcgcga tcgcctgatc ggccgcctgc
     8521 aggatcaggg cattgccagt ttcgaagtgc tcgatgccat ccgcagtatt ccccgccatc
     8581 tgttcgtgga tgaagccctg gctcaccggg cctatgaaga caccgcactg cccatcggcc
     8641 acaaccagac catctcccag ccttacattg tcgcccgcat gaccgaactg ctgattcagg
     8701 gcctgcctca gggcaagcgg ctgaagaacg tgctggagat aggcactggc tcgggttatc
     8761 agaccgccat cctgtcccgt ttcgccgaac aggtgtgctc gctggagcgg atcaagtccc
     8821 tgcaggacaa ggcgcgcaag cggctgcagg cgctgaagat ccgcaatgtg ttgctgcgct
     8881 atgccgatgg cagcgtgggc tggaaggaca aaggcccgtt cgacggcatc ctggccgctg
     8941 ccgcccccgc cgagatcccg caggaactgc tggaccagct ggccgtcggc gggcgtctgc
     9001 tgattccggt gggtgaaagc aagcaggtgc tgtatcggat tatccgcact gaaaccgggt
     9061 ttgagcgtca gaaaatggag gacgtcaaat tcgtgccatt ccggtccggc gttcgttgac
     9121 gcggtcgcac cccatcgtca gacggatacc tcgtggcggg ttacccctac cccttcagag
     9181 ctctccctcc atcttcttgc agactgggca gacggctgag agctggctat ttaatagcca
     9241 aaatgtgtac ttttgtgatg tttttgtata gattttttta tcataagtct aaatagttta
     9301 cgctgtcttt gcttctaaac ttatttaggc gattaattga actgaccaag ggcgataccc
     9361 tggagaagac ccttgtccat ggccagaaac cagaccgtcc acttcctcat cgtcctgtgc
     9421 cttggcgtca gcctcagtgc gtgcgaaacc tctcgttacc agcctacacg caatgtttac
     9481 cggtctgcag accacgccgc acaaggcttc cacaccgtca ggcccggtga aaccctgtat
     9541 tccatcgcct cccgcaatcg ccgcaatccc gacgaactgg cccgcgcaaa tggtataaaa
     9601 cccccgtacc agatctaccc tggacaaatt atatacttca ataaaaccgc ctctgccccc
     9661 gtttcccgtt caaaaccggc cgcaaaaccg cctgctacaa atcaaacaag tcgttataag
     9721 aaacccgcaa gcaataccaa accgatccaa gaaaaagttg caaatcaaag agataaaaag
     9781 gtagatatat cgtggaagtg gcctgcgggt ggtcctgtga ttgaaacttt ctccactgcg
     9841 gggcaagtca acaaggggat cgatct
//
LOCUS       DASUEG010000429         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2161097, whole genome shotgun sequence.
ACCESSION   DASUEG010000429 DASUEG010000000
VERSION     DASUEG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161097"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..521
                     /locus_tag="VFX11_08005"
     CDS             258..521
                     /locus_tag="VFX11_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4938600.1"
                     /translation="MHGWWEEAQLAPASAQADLSALIEPLTHRELEVLGMIASGHRNQ
                     AIADTLHIAVTTTKAHIRNIFEKMAVTSRTQAVARGRELGLIR"
     gene            complement(630..1394)
                     /locus_tag="VFX11_08010"
     CDS             complement(630..1394)
                     /locus_tag="VFX11_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX4938601.1"
                     /translation="MTGNTVFDVLLMAIFASALLTALVLLLVDAPYGRHKRAGWGPKI
                     NTRLGWVVMESPASLAFFYFYFTGAHPQVLAGVILMLMWQLHYVHRSFIYPFQIRVKP
                     GDSTPLLVILMGSLYCGINGYLNGSYISTYADHLNDQWLTDPRFVLGVALFGFGYYLN
                     KRSDSILRSLRASSTTGYQIPHGFAFRYVSMPNYLGEILTWTGFAIASWSLAGLSFVV
                     FTMANLVPRALANHRWYREQFKDYPRQRRAIFPFVL"
     gene            complement(1410..1835)
                     /locus_tag="VFX11_08015"
     CDS             complement(1410..1835)
                     /locus_tag="VFX11_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938602.1"
                     /translation="MSISAVTSAQSPSTGLLLRLTAWAMLVLGVSLFAAYLVFLPQPQ
                     LFAFDSQSVGASIGLILYSLATAGSAFIAWGIMLSRLDGNGLTRQQVLRATAIGIGLL
                     GLMRLGTAIFPPPPFDAMIALPIGEFVVFSLLAFKLFRS"
     gene            complement(1841..>2467)
                     /locus_tag="VFX11_08020"
     CDS             complement(1841..>2467)
                     /locus_tag="VFX11_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketosteroid-9-alpha-hydroxylase"
                     /protein_id="HEX4938603.1"
                     /translation="ELVDNMADKAHFGPVHASAALKFRNVFHKHVAVQEMEGKSPRLA
                     GESILKTTATYYGPAYMITEMTGEMNGVPVASKLLVSHVPTSTESFDLRFGVMVKLFP
                     GMSREDSDAMVKGYVQLTQQAFFEDVQFWHTKVRVDNPVLCDGDGPIHRLRQWYNQFY
                     VDIAEVPSVWDEAKVYEVEAFNPIPPQKMAAGNAPAPQSAHAGEAVEA"
BASE COUNT          569 a          764 c          718 g          416 t
ORIGIN      
        1 tgctgaatcg tcattcgcag gcactggaag tcctggacca gatcgaggaa gacgccgcca
       61 cccagcaccg gattctcacc caggtgcgcg ccctcacgac ccgcgccctg gtgcaatctc
      121 accagcagga ctgactgcgc gcctgtgcca gcctgcaacg cgcgctggag ctaagcgccc
      181 gtgcgggcta ccggcgggta tttctggacg aaggtgccgc cctggaacca ctcttcagcc
      241 ggctgtcgga gcagggtgtg cacggttggt gggaagaggc gcaactggcg ccggccagcg
      301 cacaggcgga tctgtcggcg ctgatcgaac cgctcaccca ccgcgaactg gaagtactgg
      361 gcatgattgc cagtggtcac cgcaatcagg cgattgccga caccctgcat atcgcagtga
      421 ccaccaccaa ggcccacatc cgcaacattt ttgaaaagat ggcggtgacc agccgcaccc
      481 aggccgtggc aagggggcgg gagctgggat tgatccgctg atccggcgcg gataaaaaaa
      541 acggacagtc cattggcaga gactgtccgt tgaaggagag ccggtgggaa gccggctcgg
      601 ctttggaaac ggtttgcagc gaagaacgtt tacagcacga acggaaatat cgccctccgc
      661 tgccggggat aatccttgaa ctgttcccga taccagcggt gattcgccag cgcccgcggc
      721 accagattcg ccatggtgaa caccacgaac gacaatcccg ccagtgacca ggacgcaatc
      781 gcaaacccgg tccaggtcag aatctccccc aggtaattcg gcatcgacac ataacggaac
      841 gcgaagccgt gcggaatctg gtaaccggtg gtggagctgg cgcgcagact gcgcaaaatg
      901 ctgtcggaac gtttgttgag gtagtaaccg aacccgaaca gggcaacacc cagcacaaag
      961 cgcggatctg tcagccattg atcgttcagg tgatcggcat aggtggaaat gtaggagccg
     1021 ttcaggtaac cgttgatgcc gcagtagagc gaacccatca ggatcaccag cagaggcgtg
     1081 ctgtcgccgg gcttcacccg gatctggaac gggtagatga aggagcggtg cacataatgc
     1141 agctgccaca tcagcatcag gatcacgccg gccagcactt gcggatgcgc gccggtgaaa
     1201 tagaaataga aaaacgccag tgatgccggc gactccatca ccacccagcc caggcgtgta
     1261 ttgattttcg ggccccagcc ggcgcgcttg tgacggccgt agggcgcatc caccagcagc
     1321 agcaccagcg cggtcaacaa cgcgctggca aaaatggcca tcagcagaac atcgaagaca
     1381 gtgtttccgg tcatggtggc aacccccctt caggaccgaa acagcttgaa cgccagcagc
     1441 gagaacacca cgaactcacc gatgggcagc gcgatcatgg catcaaacgg cggcggcgga
     1501 aaaatcgcgg tacccaagcg catcaggccc agcagtccga taccgatggc cgtagcccgc
     1561 agcacctgct gccgggtcag tccgttgcca tccagccggc tcagcatgat gccccaggcg
     1621 atgaaggccg acccggccgt ggccaggctg tataaaatca aaccgatgct ggcaccaaca
     1681 ctttggctgt cgaacgcaaa cagctgcggc tgcggcagaa aaaccaggta agcggcaaac
     1741 aggctgacac ccagcaccag catggcccag gctgtcaggc gcagcagtaa tccggttgac
     1801 gggctttgcg cggaagtgac ggcagaaatc gacatggaaa tcaggcctca accgcttccc
     1861 cggcatgggc ggactgcggc gccggcgcat tgcctgctgc cattttctgc ggcggaatcg
     1921 gattgaaggc ctccacctcg tacaccttgg cttcatccca caccgacggc acttcggcaa
     1981 tgtcgacgta aaactggttg taccactggc gcaagcggtg aatcgggcca tcgccgtcgc
     2041 acagcaccgg attgtccacc cgtaccttgg tgtgccagaa ctgcacatct tcgaaaaatg
     2101 cctgctgggt gagttgcaca taacccttca ccatggcatc gctgtcttcc ctgctcatgc
     2161 ccggaaacaa tttcaccatc acgccaaaac gcagatcgaa actttcggtg gaggtgggca
     2221 catggctgac cagcagcttc gacgccaccg gcacgccatt catttcaccg gtcatctcgg
     2281 taatcatgta ggcagggcca taataagtgg ccgtggtttt cagaatcgat tcacccgcca
     2341 gacgcggact cttgccttcc atttcctgca cggccacatg cttgtggaaa acattgcgga
     2401 atttcagcgc cgccgatgca tgcaccgggc cgaagtgcgc cttgtccgcc atgttgtcca
     2461 ccagctc
//
LOCUS       DASUEG010000430         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1141791, whole genome shotgun sequence.
ACCESSION   DASUEG010000430 DASUEG010000000
VERSION     DASUEG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141791"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFX11_08025"
     CDS             <1..949
                     /locus_tag="VFX11_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX4938604.1"
                     /translation="LHREAINPLERKPIDEPLDVGVRAINSVLTVGRGQRMGLFAGSG
                     VGKSVLLGMMTRFTSADITVVGLIGERGREVKEFIEHSLGADGLKRSVVVASPADDSP
                     LMRMHGAMLATAIAEYFRDQGKNVLLLMDSLTRYAQAQREIALAVGEPPATKGYPPSV
                     FAKIPQLVERAGNGREGGGSITAFYTVLTEGDDVQDPVADAARAILDGHVVLARRLAE
                     EGQYPAIDVEASISRAMPQIVTPEHLQQAQALKRLYARYQQSRDLISVGAYVAGSDPE
                     TDLAIKQIKPIRQFLAQGMLEKVDYKSACQQLQKLTTGR"
     gene            955..1398
                     /gene="fliJ"
                     /locus_tag="VFX11_08030"
     CDS             955..1398
                     /gene="fliJ"
                     /locus_tag="VFX11_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX4938605.1"
                     /translation="MMKKSQRLRPVRDLKQQQERQEAKKLADMQQQLANARRQHEELQ
                     GYLREYFNAISTTRQQVKQASQLGLYQSFITRLQDAIQHQGQLIRQREQVVQAQTRKW
                     LEASSRLKTLDDLIARARQQEELAADKREQKIQDDRPHRTPQGFE"
     gene            1576..1857
                     /locus_tag="VFX11_08035"
     CDS             1576..1857
                     /locus_tag="VFX11_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4938606.1"
                     /translation="MADATLIELDKISDISKVEALHEKLEALLGSAASVDIDAARVER
                     IDTATLQLLTVFFRALDRHHLQARILNPSRTFADTARLLGLADALNLSN"
     gene            1885..2247
                     /locus_tag="VFX11_08040"
     CDS             1885..2247
                     /locus_tag="VFX11_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938607.1"
                     /translation="MTKILAVDDSASMRQMVSFTLQGAGYEVVEACDGQEALNKAKLG
                     QVDLVLSDVNMPVMDGISLIKNLRSLPNYKFTPILMLTTESAADKKSEGKAAGATGWI
                     VKPFNPDQLLNTIKKVLG"
     gene            2253..>3131
                     /locus_tag="VFX11_08045"
     CDS             2253..>3131
                     /locus_tag="VFX11_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4938608.1"
                     /translation="MSIDLSQFHQVFFEESFEGLQLMESSLLELDCANPDGETINAIF
                     RAAHSIKGSSSTFGFGAVAEFTHVLETLLDQVRVGSRRLTPEIVDLLLRSVDCLSGML
                     RSLQAGTALDTAQSRELIAAFNAILAQQATAPAVASVPAPAVHAPAVQSPAPSGSQDW
                     LITFIPHTNILRTGNEPARMLRELSELGPLQCTALLDRLPEFTALTPEECHIGWKIHL
                     TTAEDKRKIEEIFEWVVDESDLKIELAAVEAPVQPAVVPVMAALPEAPALVALETLTE
                     PVDMADKLDTPAKQVRA"
BASE COUNT          682 a          895 c          967 g          587 t
ORIGIN      
        1 gttgcaccgc gaagcgatca atccgctgga acgcaaaccc atcgatgaac ccctcgatgt
       61 gggtgtgcgt gccatcaaca gcgtgctgac cgtggggcgt ggtcagcgca tggggttgtt
      121 tgcgggctcc ggcgttggca agagcgtgct gctgggcatg atgacgcgct tcaccagtgc
      181 cgacataaca gtggtggggc tgatcggcga acggggtcgc gaggtgaagg aattcatcga
      241 gcacagcttg ggcgctgacg gattgaagcg atcggtggtg gtggcttcgc ctgcggacga
      301 ttcgccgctg atgcggatgc acggcgccat gctggccacg gcgattgcgg aatatttccg
      361 ggaccagggc aaaaacgttt tgctgctgat ggattccctt acgcgttacg cgcaggcgca
      421 gcgggaaatc gcgctggcgg tgggcgagcc gccggctacc aagggttatc cgccatccgt
      481 gttcgcgaaa atcccgcagc tggtggaacg cgcgggtaac ggacgggaag gtggtggctc
      541 catcacggcg ttttataccg tgcttaccga aggtgacgac gtgcaggatc ccgtcgccga
      601 tgctgcccgt gccattctcg atggccacgt cgttctggcg cggcggctgg cggaagaagg
      661 ccagtatccc gcgatagacg tggaagcctc catcagccgt gccatgccac agatcgtcac
      721 gccggagcat ctgcagcagg cgcaggcgct gaaacggctg tacgcccgct accagcagag
      781 ccgcgacctg atcagcgtgg gggcctatgt ggctggcagc gatccggaaa ccgatctggc
      841 catcaagcag atcaagccga tccggcagtt ccttgcccag ggcatgctgg aaaaggtgga
      901 ttacaaatcc gcctgccagc agttgcagaa gctcaccacg gggcgctgaa ctgaatgatg
      961 aagaaatcgc agcgtttgcg cccggtgcgc gatctcaagc agcagcagga acgccaggaa
     1021 gcaaaaaagc tggcagacat gcagcagcag ctggcaaatg cccgccgcca gcacgaagag
     1081 ctgcaggggt atctgcggga gtatttcaat gccatcagca ccacccgtca gcaggtgaaa
     1141 caggcttcgc aactgggctt gtaccagagt ttcatcaccc gcctgcagga tgccatccag
     1201 caccagggcc agctgatccg ccagcgcgag caggtggtgc aggcgcagac ccgcaaatgg
     1261 ctggaagcca gttcccgcct gaaaaccctg gatgatctga ttgcccgggc gcgccagcag
     1321 gaggaactgg cggcagacaa gcgcgagcag aagatccagg acgatcgccc ccatcgcaca
     1381 ccccagggat tcgaataaca gccctccata gcggaatttg gaatctggat tgccgtctga
     1441 aatagctaaa attcataaat tccattgatc cagtgcaatt ttccgctcgc gactagcgga
     1501 agctacgcag ctgtctatcc ttctcagaca gaacggaatt ttatgtccgg cgatcagccg
     1561 ggagcggata acacaatggc agatgcaacc ctgattgaac tggacaagat cagcgacatc
     1621 agcaaggtcg aggcgctaca tgagaagctc gaagccttat tgggttcggc tgccagtgtc
     1681 gatatcgacg ccgcccgggt tgaacgcatt gataccgcca cactgcagct gctgaccgtg
     1741 ttttttcgcg cgctggaccg gcatcacctg caggcccgta ttctcaatcc ttcacgtacc
     1801 tttgccgata cggcccgttt gctcgggttg gcggacgcac tgaatttatc caactgattg
     1861 ctgtaatccg aagggagcat tcgcatgacc aaaatactgg cagtcgatga cagcgcatcg
     1921 atgcgacaaa tggtgagctt cacactgcag ggcgccggct acgaagtggt ggaagcctgc
     1981 gatggccagg aagcactgaa caaggccaag ctgggccagg tggatctggt gctgtcggac
     2041 gtgaacatgc cggtgatgga tggcatttcg ctgatcaaaa acctgcgctc attgccgaac
     2101 tacaaattca cccccattct gatgctgacc acggaatccg ccgctgacaa aaagagtgag
     2161 ggcaaggcgg cgggcgccac cggctggatc gtgaagccgt ttaacccgga tcagctgctc
     2221 aacaccatca agaaagtgct gggttaatct ccatgagtat cgatctgagc cagttccacc
     2281 aggtcttttt cgaggaaagc ttcgagggcc tgcaactcat ggaatccagc ctgctggaac
     2341 tggattgcgc caaccctgac ggggaaacca tcaacgccat tttccgtgcc gcgcactcca
     2401 tcaagggcag cagcagcacg tttggctttg gcgcggtggc ggagttcacc catgtgctgg
     2461 agacactgct cgatcaggtg cgtgtgggct cgcgccgtct cacgccagag attgtggatc
     2521 tgctgttgcg atcagtggat tgcctgagcg gcatgctgcg ttcactgcag gcaggtacgg
     2581 cgctggatac agcccaatca cgtgaattga tagctgcttt caatgcgatt ctggcgcagc
     2641 aggcgacggc accggcggtt gcctccgtgc cggcgcccgc tgttcacgcg cctgccgtac
     2701 aatcgcctgc gccatctggt agtcaggact ggctgatcac gttcattccc cacaccaata
     2761 tcctgcgcac cggcaacgaa cctgcgcgca tgttgcgtga actgtcggaa ctggggccac
     2821 tgcagtgcac ggcgctgctg gaccggttgc cggaattcac cgcgctgact ccggaagaat
     2881 gtcatatcgg ctggaaaatc cacctcacca ccgccgaaga caagcgcaag atcgaggaga
     2941 tttttgaatg ggtggtggat gaatcggatc tgaaaatcga actggccgcg gtggaagcac
     3001 cggtgcagcc tgcagtggta cccgtgatgg ccgcgttgcc ggaggcgccg gcgctggtgg
     3061 cactggagac gctcacagaa ccggtcgaca tggccgacaa actggatacg cctgccaagc
     3121 aggtgcgtgc c
//
LOCUS       DASUEG010000431         5776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1546773, whole genome shotgun sequence.
ACCESSION   DASUEG010000431 DASUEG010000000
VERSION     DASUEG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546773"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFX11_08050"
     CDS             complement(<1..302)
                     /locus_tag="VFX11_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938609.1"
                     /translation="MLHTLGKFHAGRTRALALATFTVLLAAPTQAQETATVTAQRETP
                     TLTPVDVTAEHEAEAGTIINQHRAAKTSKTRVPIQDTPASVTVVDKQFIKDSGAKN"
     gene            complement(402..812)
                     /locus_tag="VFX11_08055"
     CDS             complement(402..812)
                     /locus_tag="VFX11_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4938610.1"
                     /translation="MAFGSGLDENDEAMSEINMTPLVDVMLVLLIIFIITVPVLTHSV
                     KIDLPRADNTPNEIKPETVNLSVTADAAIHWNENVITFDELEQRLQAAATQQPQPELH
                     LRGDKTVAYEHVIKVMAAAQRAGVHKLGFVTEPN"
     gene            complement(814..1551)
                     /locus_tag="VFX11_08060"
     CDS             complement(814..1551)
                     /locus_tag="VFX11_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4938611.1"
                     /translation="METNPYGIEALWLQGDPVIKAVAVILLLMSIASWYVIVVRSWNL
                     LSLRRVQRALGDFWHARSLAEGLHMLGNEGDHNPFRMLAEAGQAAVDHHQNHKSDLHG
                     QLLLADWLTGCLRGSIDESAERLQKGLSVLASVGSTAPFVGLFGTVWGIYHALVGIGV
                     SGQASIDKVAGPVGEALIMTAFGLAVAIPAVLGYNALTRNNKMVLGKLNRFAHQLHSY
                     FITGTPLTRNKQPLVKISHIAPKQGGN"
     gene            complement(1577..2278)
                     /locus_tag="VFX11_08065"
     CDS             complement(1577..2278)
                     /locus_tag="VFX11_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4938612.1"
                     /translation="MKTGPRSAVVLTLVVLAHAGALAAAVLLQQTPEPPLELPVISGV
                     IIEAPPAEVVEAPSARVAPPEPKPVEQPKPKPQPKPQPKPKPKPVPPPPVVPPPPSEN
                     AIATPPPPEPVQQQAAPPPPAPSTPQAEEKDTLGAPVQPPRIDAKQRNNPAPAYPPLS
                     KRRGEEGVVILELLITAEGRVSDLKVSQSSGFPRLDQAALDAVERWRYEPARRNGVAI
                     EYRYLQPVRFALDKR"
     gene            complement(2291..2734)
                     /locus_tag="VFX11_08070"
     CDS             complement(2291..2734)
                     /locus_tag="VFX11_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938613.1"
                     /translation="MATRTFWQRWRYRINGLLLLAPCWFLYDALTPTFPPEWPEQPIG
                     PFSAAPRPADDNPPYPHDGGYLKDFSVRFCDGCVTKIRTAHVSVGLQPLEQPATEGLL
                     HGSSRSQHVHVPYPPSQGAGEMLWVSVQEWNGRMHHAAWPLPQNQ"
     gene            complement(2738..3931)
                     /locus_tag="VFX11_08075"
     CDS             complement(2738..3931)
                     /locus_tag="VFX11_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX4938614.1"
                     /translation="MASSNRRLWFLFHGWLSLPVWLLFSFICLTGTIAVFSHEITWLA
                     NPAARAGNPDNLPRQPLPALVAAAQQAVPGAEISRIMVLEPYLVTAVALSAPDTPNAI
                     AYVNPYTAQVQAVEQGMTFIGFMRSLHGWLLFPWQHSYSIGYYLVGAMSLVVLGAMIT
                     GLVVYKRFWRAFTRPRLRLDRDARTWLGDLHRLGGVWSLWFLLVIGLTGFWYLVQAVL
                     WHTGVHVWPEAEPVALNDVPLTDGTPPAMIPLQQVLAAAQAQLPQLEPALVSMPEHNR
                     DYFSVLGSGDAILFDRYSFRVYINPWTGAVAQSHTPADMNLLQTFTHIADPLHYGTIG
                     GLWTKTIWFLFGLMLSGMSISGFLIYGKRTVKGVRAGRTKARKPVVAAPGTLPDKSPG
                     QLEGA"
     gene            complement(4056..4352)
                     /locus_tag="VFX11_08080"
     CDS             complement(4056..4352)
                     /locus_tag="VFX11_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEX4938615.1"
                     /translation="MLTSEASVATVNASLYLKKLCKHFQHKVEAQWDDHQGIAHFPMG
                     IGTFAATADRLTMRCQAETQEALEKVQWIIDDHLIRFARKENLVPQWRGLVAGQ"
     gene            complement(4438..5637)
                     /locus_tag="VFX11_08085"
     CDS             complement(4438..5637)
                     /locus_tag="VFX11_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938616.1"
                     /translation="MPRYMTITAPGRVILLIAAVKFVYILDFMMLMPLGPDLSRALGF
                     PAERLGWLSAAYTAASMLAGIVAVRWLDRFDRKPVFLLFWGLLVLATAATALATDMTQ
                     LLLLRAFTGLVGSPAVALGMAIVIDCIPLPQRGRAIGKVMIGFSLAAVLGIPLALELA
                     RLGDWRSPFWLVALGAMLVWFAALVILPDLNAHRADAGADEVSPLSLLRQPAVRKACL
                     IQAGSQFSAFLIVPHFSAFFLLNLQFPREYLGSLYLAGGIAALLMMQWLGRMTDSAGP
                     ARAVLIATACTVAGLTPWLGFASLPLLIPFVLFMAGNAGRNVSIAAVTSQVPAPHERA
                     GYIALEGMVQDLAITAAALVASAILGSGSSGELTHTTPLAVLAVGLTLSTLFALTRWQ
                     PALLRGN"
BASE COUNT         1101 a         1836 c         1798 g         1041 t
ORIGIN      
        1 atattcttcg cgcccgaatc cttgatgaac tgcttgtcca ccacggtgac cgacgccggc
       61 gtgtcctgga tcggaacgcg ggtcttgctg gttttggcgg cgcggtgctg attgatgatg
      121 gtgccagctt ccgcttcatg ctccgccgtc acgtccaccg gtgtcagcgt gggcgtttcg
      181 cgctgggcgg tgacggttgc ggtttcctga gcctgtgttg gcgcggcaag cagaacggta
      241 aaggtggcca atgccagagc cctggtgcgc ccggcatgaa atttccccaa tgtgtgcagc
      301 atgtttgatc actcccgatg aaagaggatc aaaccttaat gagaattatt attactagca
      361 ataatatttg agtgattcgc aaccattttc cggatcagtg gtcagttcgg ctccgtcacg
      421 aaacccagtt tgtgcacgcc ggcccgctgc gctgccgcca tcaccttgat cacgtgctcg
      481 tatgccaccg ttttgtcgcc gcgcaggtgc agttccggtt gcggttgctg ggttgcggcc
      541 gcctgcaggc gctgctccag ctcgtcgaag gtgatcacgt tttcgttcca gtgaatcgcg
      601 gcatccgccg tcaccgacag gttgacggtt tccggcttga tctcgttggg tgtgttgtcg
      661 gcccggggca gatcgatctt caccgagtgg gtcagcaccg gcacggtgat gatgaagatg
      721 atcagcagca ccagcatgac atccaccagc ggcgtcatgt tgatttcact catggcctcg
      781 tcgttttcgt ccagaccgct gccaaacgcc atctcagttg cctccctgct tgggcgcgat
      841 atgggaaatc ttcaccagcg gctgcttgtt gcgggtcagc ggcgtgccgg tgatgaaata
      901 ggaatgcagc tggtgcgcaa accggttcaa cttgcccagc accattttgt tgttgcgagt
      961 gagcgcgttg taacccagca cagcgggaat cgccaccgcc agaccgaacg ccgtcatgat
     1021 cagcgcttcg cccaccgggc ccgccacctt gtcgatgctg gcctggccgg acacaccaat
     1081 gcccaccaac gcgtgataaa taccccacac ggtgccgaac agtcccacga acggcgcggt
     1141 ggagcccacc gaggccagta ccgacaatcc tttctgcagg cgctcggcgc tttcgtcgat
     1201 ggagccgcgc aggcagccgg tgagccagtc cgccagcaac agttggccat gcagatcgct
     1261 cttgtgattc tggtggtgat cgaccgcggc ctgccccgct tccgccagca tgcggaacgg
     1321 attgtggtcg ccctcgttac ccagcatgtg caggccctcg gccagactgc gcgcatgcca
     1381 gaaatcaccc agcgcacgct gcacgcgacg cagtgacagc agattccagc tgcgcaccac
     1441 aatcacgtac caggacgcga tggacatcag cagcaggatg accgccactg ccttgatgac
     1501 cggatcgccc tgcaaccaga gcgcttcgat gccgtaggga ttggtttcca tagtgatctc
     1561 cttcatgtgt tcagaatcat cgcttgtcca gcgcgaaacg cactggctgc aggtaacggt
     1621 attcgatggc gacgccgttg cgccgcgccg gttcgtagcg ccagcgctcg acagcgtcca
     1681 gcgccgcctg atccagccgc gggaatccgc tggactggct gaccttgaga tcggacaccc
     1741 gcccttcggc ggtgatcagc agttccagga tcaccacgcc ctcttcgccc cggcgcttgg
     1801 acaggggcgg ataggccggc gccggattgt tgcgctgttt ggcgtcgatc cggggcggtt
     1861 gcaccggggc gcccagggtg tctttttcct cggcctgcgg tgtcgagggc gcgggaggcg
     1921 gtggtgcggc ctgctgctgc accggttccg gtggcggcgg cgtggcgatg gcgttctccg
     1981 acggcggagg cggcaccacc ggcggcgggg gcaccggttt gggttttggc ttgggctgcg
     2041 gttttggctg gggttttggc ttgggctgct ccaccggctt gggttccggc ggcgccaccc
     2101 gggcactggg ggcctccacc acttcggcgg gcggggcctc gatgatcacg ccgctgatca
     2161 ccggcaattc cagcggcggc tcgggcgtct gctgcaacag tacggcggcc gccagcgcgc
     2221 cggcgtgggc gagtaccacc agcgtcagta cgactgcact tcttggccct gtcttcaact
     2281 gcttacccct ttactgattc tgcggcaatg gccaggcggc atgatgcatc cgcccgttcc
     2341 actcctgcac actgacccac aacatctcac ccgccccctg agagggtgga tagggtacat
     2401 gcacatgctg gctgcgggaa ctgccgtgca gcaggccttc ggtggcaggc tgctccagtg
     2461 gctgcaggcc cacgctgacg tgggcagtcc gtatcttcgt cacacagcca tcgcaaaaac
     2521 gcacgctgaa atccttcagg tagccaccat cgtggggata gggcgggttg tcatccgccg
     2581 gccggggcgc ggcgctgaaa gggccgatcg gctgctccgg ccactccggc ggaaaggtgg
     2641 gcgtgagcgc gtcgtataga aaccagcaag gcgcgagcag cagcaggccg ttgatacggt
     2701 agcgccagcg ttgccagaag gtgcgggttg ccatccgtca ggctccttcc agttggcccg
     2761 gcgacttgtc cggcaaagta cccggcgcgg ccacgaccgg tttgcgtgct tttgtccggc
     2821 cggcccgcac gcctttcacc gtgcgcttgc cataaatcag aaaaccgctg atcgacatgc
     2881 cggacagcat cagcccgaac agaaaccaga tcgtcttggt ccagaggcca ccgatagtgc
     2941 cgtaatgcag cgggtcggcg atgtgggtga aggtctgcag cagattcatg tccgccggcg
     3001 tgtgggactg cgccactgcg ccggtccagg gattgatgta gacgcggaag gaatagcggt
     3061 cgaacaggat ggcatcgcca ctgcccagca cgctgaaata atcgcggttg tgttccggca
     3121 tggacaccag cgccggctcc agttgcggca attgagcctg ggccgccgcc agtacctgct
     3181 gcagcggaat catcgcgggc ggcgtaccat cggtgagcgg cacatcgttc agcgccaccg
     3241 gctccgcttc cggccataca tgcaccccgg tgtgccagag caccgcctgc accaggtacc
     3301 agaacccggt cagcccgatc accagcaaaa accacaggga ccagacgccc cccagccggt
     3361 gcagatcccc cagccacgta cgggcatccc ggtccaggcg caggcgcggc cgggtgaagg
     3421 cgcgccagaa acgcttgtac accaccagcc cggtgatcat ggcgcccagc accaccagcg
     3481 acatcgcacc caccaggtaa tagccaatgc tgtaactgtg ctgccagggg aacagcagcc
     3541 agccgtgcag cgaccgcatg aaaccgatga aggtcatgcc ctgctccacc gcctgcacct
     3601 gtgcggtgta gggattcaca taggcgatgg cattgggcgt gtccggcgcc gacagcgcaa
     3661 cggcggtgac cagatacggc tccagtacca tgatgcggct gatctcggca cccggcaccg
     3721 cctgctgcgc ggccgccacc agcgccggca agggctggcg gggcagatta tcggggttac
     3781 cggcgcgtgc ggcggggttc gccagccagg tgatttcatg gctgaacacc gcgatggtgc
     3841 cggtcaggca gatgaagctg aacagcagcc acaccggcag cgacagccag ccatgaaaca
     3901 ggaaccagag gcgacggttg gaggaagcca taatcaaaaa ccttgcagga cggatcggtg
     3961 ccggtgcatg tcatctgttc aattaatgaa cctgacacaa atataaatga taatgattag
     4021 caactctacc tgattttgta acaattcggc tacagctatt ggccggccac cagcccacgc
     4081 cactgcggca ccagattctc cttgcgggca aaccggatca ggtggtcgtc gatgatccac
     4141 tgcacttttt ccagggcctc ctgcgtttct gcctgacagc gcatggtcag ccggtccgcc
     4201 gtcgcggcaa acgtgccaat gcccatggga aagtgggcga tgccctggtg gtcgtcccac
     4261 tgcgcctcga ccttgtgctg gaagtgcttg cagagttttt tcagatagag actggcgttg
     4321 acggtggcaa cgctggcttc gctggtcagc atgggtagtg gctcgcagga tagtggcttg
     4381 caggttgaag gacagatcgg gaaatggaag cgcgcaggcg acagacatcg gccatgctca
     4441 gttgccccgc agcaaggccg gttgccagcg ggtcagggca aacagggtgc tcagggtcag
     4501 gccgacggcc agcaccgcca gcggcgtggt gtgggtgagt tcgccactgc tgccactgcc
     4561 cagaatggcg ctggccacca gcgcggcggc ggtgatcgcc agatcctgca ccatgccttc
     4621 cagcgcgata taacccgccc gctcgtgggg cgccggcacc tggctggtga cagcagcaat
     4681 gctgacattc cgccccgcat tgcctgccat gaacagcaca aacggaatca gcagcggcag
     4741 gctggcgaat cccagccagg gggtcagccc cgccactgtg caggcggtgg cgatcaacac
     4801 ggcacgggca ggtccggcac tgtcggtcat gcgccccagc cactgcatca tcagcaacgc
     4861 ggcaatgcca cctgccaggt acagcgaccc caggtattcc cggggaaact gcagattcag
     4921 cagaaagaag gcggaaaaat gcggcacgat caggaaggcg gaaaactggc tgccggcctg
     4981 gatcagacat gccttgcgca ccgccggctg gcgcagcagg ctgaggggcg acacctcgtc
     5041 agcgcccgcg tcggcacggt gcgcattcag gtccggcaga atcaccagcg ccgcgaacca
     5101 caccagcatg gcccccagcg ccaccagcca gaacggcgag cgccagtccc ccagccgcgc
     5161 cagttccaat gccagcggaa tccccagcac tgctgccagc gagaagccga tcatcacctt
     5221 gccaatcgcc cgcccccgct gcggcagtgg aatgcagtcg ataacgatgg ccatgcccag
     5281 cgccacggcc gggctgccca ccagtccggt gaatgcgcgc agcaataata gttgtgtcat
     5341 atccgtggcc agcgccgtgg ccgccgtggc cagcaccagc agcccccaga acagcaggaa
     5401 aacaggcttg cggtcgaaac gatccagcca gcgcacggcc acgatccccg ccagcatcga
     5461 cgccgcggtg taggctgccg acagccagcc cagccgttcc gccggaaaac ccagcgcgcg
     5521 ggacagatcc ggccccagcg gcatcagcat catgaaatcc aggatataga cgaacttgac
     5581 cgccgcgatc agcaggatga cgcggccagg tgcagtgatg gtcatgtaac ggggcatgcg
     5641 gcggccttat gaatttttat aaatgctatt gataacgatt atcattattg atattttact
     5701 gaacgccgag gtcccaatgc aaccccttgt ttgccggtga atttttaccc agccacttcg
     5761 ttctgcaagg attcag
//
LOCUS       DASUEG010000432         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1604397, whole genome shotgun sequence.
ACCESSION   DASUEG010000432 DASUEG010000000
VERSION     DASUEG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604397"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFX11_08090"
     CDS             complement(<1..544)
                     /locus_tag="VFX11_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938617.1"
                     /translation="MHIQKMGWLLIGFALLAGCKLQTQGSIDLRDGAGEPYSFAGAAV
                     TLFPLNAAAVMPGQSKTVLATGTVAPEDGAVTLTANRTVVRDSRLYAVQFTCPPNVPD
                     DDCEVATPVHVVLTEADLKAGGWRATALTEAAFHAVSYGAASRFSAADLQQVLDEASQ
                     ALLALPYQDLLQWNPDDTTAL"
     gene            complement(562..>2093)
                     /locus_tag="VFX11_08095"
     CDS             complement(562..>2093)
                     /locus_tag="VFX11_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938618.1"
                     /translation="AFISQRGVYTGNPGFAGLQIVDISNPQAPVQVGSLAIASGLVHL
                     AVSGNRVYATGDGGLYVIDFSNPAAPTLAGNLAFDNALNAIAVAGQYAYTEGAGVLRI
                     IDIGNPSLPVVGSLSGTWGNDINLVGSRLYIGADVIDISSPFTPQRIGRIPLSGNAQF
                     VLPDGLAYSAAGASLNIYDTNSVDRAPLLAGSAEVNFEMLVGLPGNTVAENGFAFAPN
                     ITAGIAVFDLADPLSPHYVGTDMFSDYISELALAGPYAYGGDVESGSLRIIDIANPAG
                     LEFEFPLVGAIQFSDSERFNPKSVVVAGPYAYVAYSEAVEDPETGETIRTGGLYVVDI
                     QTPSDPQIAAHYTGSTFADALAVQGNYAYLMFSDGSLDVIDISNPLAPVLLATTFLPE
                     PVDFLKVSGDVLYAPAGALGLHILALANPALPTLLTTLDTPGYARRMVVEDDVAYVSD
                     GSSGVQVVSVGNPAAPVLIGAANTIGSADGVFLHDEYLYANTSYGFEILRKMPVITPA
                     P"
BASE COUNT          441 a          651 c          612 g          389 t
ORIGIN      
        1 gcagtgcggt ggtatcgtcc gggttccact gcagcagatc ctgataggga agggcgagca
       61 atgcctgcga agcctcgtcc agaacctgct gcagatccgc cgcgctgaat cttgatgcag
      121 cgccatagga caccgcgtgg aatgccgctt ccgtcagcgc ggtggcgcgc cagccgccgg
      181 ctttcagatc ggcctctgtc agcactacgt gcacgggcgt ggccacttcg caatcatcgt
      241 caggcacatt gggcgggcag gtgaattgca ccgcatacag ccggctgtcc cgcacaacag
      301 tgcggtttgc ggtcaacgtc actgcaccat cttccggtgc caccgtgccc gtcgctaaca
      361 ctgttttgga ctgacccggc atcacagcgg cagcattcag cgggaacagc gtaactgccg
      421 caccggcaaa ggaatagggt tcacccgcac cgtcacgcag atcgatgctg ccctgtgtct
      481 gcaatttgca accggccagc aaggcaaagc cgatcagcaa ccaacccatt ttttggatgt
      541 gcatatccgt tctccctgat gtcacggtgc cggagtgatc accggcatct tcctgagaat
      601 ttcaaaaccg taactggtat tggcatacag atattcgtcg tgcagaaata cgccgtcggc
      661 ggaaccgatg gtgttggccg cgccaatcag caccggtgcc gccgggttgc cgacggacac
      721 cacctgcacg ccactgctgc cgtcagacac ataggcaacg tcgtcttcca ccaccatccg
      781 gcgggcgtac ccgggtgtgt ccagtgttgt cagcagtgtt ggtaatgcgg gattggcgag
      841 cgccagaata tgcaggccca gggctcctgc gggcgcgtac agcacatcgc cggacacctt
      901 caggaaatcg acgggctcgg gcaggaacgt ggtggcaagc agcaccggcg ccagcggatt
      961 gctgatgtcg atcacatcca ggctgccatc gctgaacatc agataggcat aattgccctg
     1021 cacggccagc gcgtcagcaa aggtcgagcc ggtgtaatgc gccgcgatct gcggatcgga
     1081 cggcgtctgg atatcgacca cgtacagtcc accggttcgg atggtttcgc ccgtttcggg
     1141 atcttccacc gcctcgctgt aggcaacgta ggcatacggg cccgccacca cgacggattt
     1201 tggattgaag cgctcactgt cactgaactg gatcgcgccc accagcggaa attcaaactc
     1261 cagccccgcc ggattggcga tatcaataat tcgcagcgat ccggactcca catcaccgcc
     1321 ataggcatag ggacccgcaa gcgccaattc gctgatgtaa tcactgaaca tatcggtgcc
     1381 aacataatgc ggcgacagcg gatcagccag atcgaacacc gcgatacccg ctgtgatgtt
     1441 cggtgcaaag gcgaaaccgt tttccgccac ggtattgccg ggcaggccga caagcatttc
     1501 aaaattcact tccgcgctgc cagccagcag cggggcccgg tcaacactgt tggtatcgta
     1561 aatgttcagg ctggcgccgg ctgcgctgta ggccaacccg tccggcagaa cgaactgcgc
     1621 atttccactc agtggaatac ggccgatccg ttgcggcgtg aaaggcgaac tgatgtcgat
     1681 aacgtcggcg ccgatataca ggcgactgcc caccaggttg atgtcgttgc cccaggtgcc
     1741 ggacagactt ccgaccaccg gcagggaagg attgccgatg tcgatgatgc gcagcacacc
     1801 cgcgccttcc gtgtaagcgt attggccggc aactgcgatg gcattcaggg cgttgtcgaa
     1861 cgccaggttg cccgccagtg tgggcgccgc cgggttgctg aagtcgatga cataaagtcc
     1921 accatcccct gtcgcgtaaa cccgattgcc ggacaccgcc agatgcacca gccctgacgc
     1981 aatcgccagg ctgcccacct gcaccggtgc ctgcggattg ctgatatcga cgatctgcaa
     2041 accggcgaaa ccgggattgc cggtatagac gccgcgttgg gaaataaacg cgt
//
LOCUS       DASUEG010000433         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1402471, whole genome shotgun sequence.
ACCESSION   DASUEG010000433 DASUEG010000000
VERSION     DASUEG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402471"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFX11_08100"
     CDS             <1..635
                     /locus_tag="VFX11_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX4938619.1"
                     /translation="IGCDGADVAAVTAQVVANKRFIMTTPGIQAFDPAAVGGGAVVTL
                     SGTYSAAALAGLISSLPVQNSPTNKVIPGITKLGSRYSYSQTVTLLNNDVVPLEERNG
                     VRVVRGITTEGEAFKQITTRRIVDFAKAGVRQASQPFIGRLNNERVRKSLQGAIDGFL
                     SNMVVNESLIAYELEVKASRQDEINGRCIVNVAMQPVFSIDYIHVTLVLS"
     gene            648..1142
                     /locus_tag="VFX11_08105"
     CDS             648..1142
                     /locus_tag="VFX11_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938620.1"
                     /translation="MANANIYTGADGSISLSSPEGLEGEKAQAVIDANDLVSVGRVQG
                     VKVEVSSDLKPFHEIGTRYATELRAGNITITGSIDRAFVNGALLKLLLGDAAEQRPAG
                     NWNQPAFNISLLVENVARPGVRNTVTLHGVKISNWNYQIPEDDFVMEAARFQALSITV
                     GDEG"
     gene            1142..1615
                     /locus_tag="VFX11_08110"
     CDS             1142..1615
                     /locus_tag="VFX11_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938621.1"
                     /translation="MLSADELLAGGGLTFSVEVPPHILQPGGGVAPVAAGSVQLRPLT
                     VKDLQRISRAAKDSDNLTATLMVQAALVEPALSVQQVAALHVGLVDFLLEQVNRISGI
                     ASTPEDIGQAASDPLAKAAFILAREFGWTPQDINNLTLGQVVLHLEMLKQQQANP"
     gene            1632..>2066
                     /locus_tag="VFX11_08115"
     CDS             1632..>2066
                     /locus_tag="VFX11_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938622.1"
                     /translation="MAEKLRAEPIRAVRCLLGRLNQSGTQANGRRSALDETALQGWIN
                     RLDPALALCGFHPFAQTDRLPAVERVVAPSGSVPATDTAQDFFQTLTRLVQETQAVKG
                     GAMPASGRKNRLGAETVATNAKNSSGSRQVGQASAQSLKPGSA"
BASE COUNT          422 a          582 c          693 g          369 t
ORIGIN      
        1 tgatcggctg cgatggcgct gatgtggcgg ccgttaccgc gcaggtggtg gcgaacaagc
       61 gcttcatcat gaccacgccg ggaattcagg ctttcgatcc ggcagcggtg ggcggtggtg
      121 cggtcgtaac attgagcggc acctactccg ccgccgcgct ggcgggattg atcagcagcc
      181 tgccggtgca gaacagcccc accaacaagg tgattcccgg cattaccaaa ctgggcagcc
      241 gctattccta cagccagacc gtgacgctgc tgaacaacga cgtggtaccg ctggaagaac
      301 gcaacggtgt gcgcgtggtg cggggcatta ccaccgaagg cgaggcgttc aagcagatca
      361 ccacccgccg catcgtggat ttcgccaagg ccggcgtgcg tcaggccagc cagcccttca
      421 tcggccggct caacaatgaa cgggtgcgca aatccctgca gggtgccatc gacggtttcc
      481 tcagcaacat ggtggtgaat gaatccctca tcgcctatga gctggaagtg aaggcgagcc
      541 ggcaggatga aatcaacggc cgttgcatcg tcaacgtcgc catgcagccg gttttcagca
      601 tcgattacat tcacgtgaca cttgttttgt cgtaaggaga cactgccgtg gccaatgcca
      661 atatttacac cggagccgac ggttccatca gcctgtccag cccggaagga ctggaaggcg
      721 aaaaagcgca agcggtcatc gacgccaacg atctggtgtc ggtgggacgg gtgcagggtg
      781 tgaaagtgga agtcagttcc gatctgaaac cctttcacga aatcggtacc cgctacgcca
      841 ccgagctgcg cgcgggcaac attaccatca ccggctccat cgaccgcgcg ttcgtcaatg
      901 gcgcgctgtt gaagctgctg ctgggtgatg cggcggagca gcgcccggcc ggcaactgga
      961 accagcctgc gttcaatatt tccctgctgg tggaaaacgt ggcgcggccc ggcgtgcgca
     1021 acacggtgac gctgcacggc gtgaaaatca gcaactggaa ttaccagatt ccggaagacg
     1081 atttcgtcat ggaagcggcg cggttccagg cgttgtccat taccgtgggt gacgaaggct
     1141 gatgttgagc gcagacgaac tgctcgccgg cggcgggctg actttctcgg tggaggtgcc
     1201 gccccacatt ctgcagccgg gcggtggcgt cgcgccggtt gcggcgggca gtgtgcagct
     1261 gcgaccactg acggtgaagg atctgcagcg gatttcccgc gccgccaagg acagcgacaa
     1321 cctcaccgcc acgctaatgg tgcaggcggc actggtggag ccggcgctgt ccgtgcagca
     1381 ggtggcggca ctgcatgtgg ggctggtgga ttttctgctg gagcaggtca atcgcatcag
     1441 tggcattgcc agcacgccgg aggatatcgg ccaggcggcc agtgatccgc tggcgaaggc
     1501 cgcattcatt ctggcgcggg aattcggctg gacgccgcag gacatcaaca atctgacgct
     1561 gggtcaggtg gtgctgcatc tggaaatgct gaagcagcag caagcgaacc cataacattc
     1621 aaggcaatac agtggcggaa aaactgcgag cagaacctat cagggcggtg cgatgcctgc
     1681 tgggccggtt gaatcaatcc ggcacgcagg cgaatggcag gcgttctgcg ctggatgaga
     1741 cggcgttgca gggttggatc aaccggcttg atccggcgct ggcgttgtgc ggctttcatc
     1801 cctttgcgca gacggaccgg ttgccggcgg tggaacgcgt ggttgcgcca tcgggttccg
     1861 tgccagcgac cgacaccgcg caggattttt ttcagacgct gacgcggctg gtgcaggaaa
     1921 cgcaggcggt gaaaggtggg gcaatgccgg caagcggaag gaagaacagg ctgggagcgg
     1981 agacagttgc gacgaatgca aaaaattcgt ctgggtcgcg gcaagtcggg caggcttccg
     2041 cgcaatcgtt gaaaccaggc agcgca
//
LOCUS       DASUEG010000434         6712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1369765, whole genome shotgun sequence.
ACCESSION   DASUEG010000434 DASUEG010000000
VERSION     DASUEG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369765"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /gene="pyrG"
                     /locus_tag="VFX11_08120"
     CDS             complement(<1..175)
                     /gene="pyrG"
                     /locus_tag="VFX11_08120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431908.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HEX4938623.1"
                     /translation="MTKYIFVTGGVVSSLGKGIAAASLASILEARGLKVSMMKLDPYI
                     NVDPGTMSPYQHGE"
     gene            complement(317..1654)
                     /gene="tilS"
                     /locus_tag="VFX11_08125"
     CDS             complement(317..1654)
                     /gene="tilS"
                     /locus_tag="VFX11_08125"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4938624.1"
                     /translation="MDATLIDQALAARPGPPAQPPPARLVVGFSGGLDSTVLLQLARQ
                     WRHHHSSTLLLALHINHQLHPHADDWQWQCAELCKHWQVPFVAQAVAIDRTRSSLEQS
                     ARAARYAAFHQHCQPGDVLLLAHHRDDQVETLLQRLLRGSGPLGLGGMGVFSQQQGLT
                     ILRPLLDIDRTDLEQHAREHQLRWIDDPSNADSRFERNFLRHDALPLLRSRWPQLNQT
                     LARSARLNREAAELLDEMAQGDQGEPATDGGLSLARLNNLSEARARNLIRYWLRQQGA
                     SLPSEVQLQRVLDEIVPAADDARPELVWGGQSIRRYRDALFCVPDLPPPAPHPQAFDP
                     SRPQQTRLAGTLVGIEGQGTAFSRTALQTGPLTLRFRQGGETAKPAGRHTRSLKQWLQ
                     DHRVPPWWRDHWPILYVNDQLAGLPGLFVCEGFQPPTPADALWFDWQPPRLQG"
     gene            complement(1682..3658)
                     /locus_tag="VFX11_08130"
     CDS             complement(1682..3658)
                     /locus_tag="VFX11_08130"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX4938625.1"
                     /translation="MRVNKWFSGAGLILLALVVLFSTLFTDWLFKGVRFDLTEGNLYT
                     LSDGSKQIVRDIQQPVELQFYFSQEATRDAQGWRTYAKQVRELLEEFALASNGKLKLH
                     VIDPEPFSEEEDRAAAFGLQAINAGGGEEKVFFGLVAQVQPPKAEGDANQDGDGKTAE
                     EKPEPRTEVIPVFQPDRQEFLEYDIAKLVYQVSQQQKPKVALLTDLEVQGGFDMMMRQ
                     PSQPWATISQLEQLFDVQKLTGTEQEISTTDYQLLLVIHPKNLSDAMLFAIDQYVLRG
                     GHALIFVDPFAEQEKGLTSTDRASDLNRLFKAWGVDYDKTKVLGDAQLALQVGMGQGR
                     APVRHLAILQLSDRNLARDNMIVSKLETLHMSSVGFVAHAKDAKTTFEPLLSSSEHAM
                     AFDAEKLAMMQDPRELTQGFTPSGTPFVLGARVTGDVQSAFPDGVPQSEGAAEAGQGK
                     PDAKVTEKTPAKADDEYLKASLKPINVVIVADTDMLTDRLWVQVHQFFGQQVAQPFAD
                     NGDFFFNSVDVLSGSADLIGIRGRGRFFRPFTVVQDMQRNAEASFRQVQESLTARLQE
                     IEGKLAELQQKQGEDGNVITLTPEMQSDLLKFQQEKLQIRKQLRDVQHQLNQDIDRLD
                     FRLKLINIAGVPLLLTLLAIVIAVYRRKQMRRIA"
     gene            complement(3674..4408)
                     /locus_tag="VFX11_08135"
     CDS             complement(3674..4408)
                     /locus_tag="VFX11_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4938626.1"
                     /translation="MNNLTVILKRELASYFATPLAYVFILIFLVLAGSFTFFFGNFFQ
                     GGQADLRGFFAYHPWLYLFLVPALSMRLWAEERKSGTLELLMTLPITLRDMVLGKFLA
                     AWVFTGIALVLTFPIWITVNYLGDPDNGVIVASYLGSWLMAGGLLAIGSCISATTKNQ
                     VIAFIISVVVCFLFMVSGMEIVQGWLPQWMPESVSTAVAALSYITHFNSISKGVLDLR
                     DVFYFVFLIGGWLIANAIVIDMKKAN"
     gene            complement(4408..5328)
                     /locus_tag="VFX11_08140"
     CDS             complement(4408..5328)
                     /locus_tag="VFX11_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938627.1"
                     /translation="MIRIDNLTKKFGQFTAVNGLSFTVAPGEVLGFLGPNGAGKSTTM
                     KMITGFLAPTSGQVTIMGHDVAHHPVKAKQLIGYLPEGAPSYGDMTVLQFLRFIGQVR
                     GYRGAELAARVERCQHALQLDKVLNQPIETLSKGFRRRVGLAQAIIHDPQVLILDEPT
                     DGLDPNQKHEVRNLIANLSKDKIVIVSTHILEEVSAVCSRAIIIAGGKILADGTPAQL
                     EARSRYHGAVTLGLEHASNAQQILSALPQVDHIEAGEDHTLIVFPKAGQQLFEVVNNA
                     IQQHQWQVRELHVERGRLDEVFRDITREVA"
     gene            5728..>6712
                     /locus_tag="VFX11_08145"
     CDS             5728..>6712
                     /locus_tag="VFX11_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4938628.1"
                     /translation="MVRTQVQPDDISRLNLDPARPVCYVLKNESLADYLVADQECRNL
                     GLPRPSDGFSLGTFNQRHGVFFLTERGHFWQRKAMLQRQPKKLLAIVEWLEQNPQQDI
                     QLVPVSVFWGRAPDRENSIFKILFSDSWGVPGFFRKLFIIFAQGRQTLVYFSEPVSVR
                     TVLTEGLAKERTARKISRVLRTHFRRLRETVIGPDLSHRRTLVNAILRMPSVAAEIEA
                     ETRRNKLTYKKAFDNARSYAEEIAADYSHAVIRLYLLVLTWLWNRLYDGVQVHGLDRL
                     YKVAKDNEIIYVPCHRSHIDYLLMSYVLFVNGLVPPHIAAGVNLNMPVVGPI"
BASE COUNT         1309 a         2064 c         2015 g         1324 t
ORIGIN      
        1 cttcaccatg ctgatacggg ctcatggtgc ccgggtcgac gttgatgtag gggtccagtt
       61 tcatcattga aactttgagc ccgcgcgctt ccagaataga ggccagagag gcagctgcga
      121 ttcccttacc cagggaagac acaacaccac ccgtcacaaa aatgtatttt gtcatcgacg
      181 taccagtgtg agcggagaat ggaaacaggt tgcggcgacc cggcgggcca cggagccgga
      241 ctcagatggc cagactcagg atgggaggca agtttacccg atcagggcgc ctgactcaac
      301 gcgacggatc atggaatcag ccctgtaagc ggggtggttg ccagtcgaac cagagggcgt
      361 cggcaggcgt cggcggctgg aatccttcgc agacgaacag gcccggcagg cccgccagct
      421 gatcattcac gtacaagatc ggccagtggt cgcgccacca gggcggcacc cggtggtcct
      481 gcagccactg tttgagggaa cgggtatgac ggccggcggg tttggccgtt tccccgccct
      541 ggcggaaacg cagggtgagt gggccggtct gcagggcggt gcgggagaaa gcagtgccct
      601 gcccttcgat gccgaccagc gtccctgcca gacgggtttg ctgcggccga gacggatcaa
      661 acgcctgtgg atggggtgcc ggtggcggca gatccggcac gcaaaacagg gcatcccggt
      721 agcggcggat ggactgcccg ccccagacca gctccggccg ggcatcgtcg gcagcgggga
      781 cgatttcatc cagcacccgc tgcagctgga cctcgctggg caaggaggcg ccctgctgcc
      841 gcagccagta gcggatcagg ttgcgggcac gggcctcgct caaattgttc aggcgcgcca
      901 gcgacaggcc gccgtctgtt gccggttcgc cctgatcgcc ttgcgccatt tcatccagca
      961 gctctgctgc ttcccggttc aggcgggccg accgtgccag ggtctggttc agctgcggcc
     1021 agcgggaacg cagcagcggc aatgcgtcgt ggcgcaggaa attgcgttcg aagcggctgt
     1081 cggcgttgga cgggtcgtca atccagcgca actggtgctc gcgggcgtgc tgttccaggt
     1141 cggtccggtc gatatccagc agcggccgga ggatagtcag tccctgctgc tggctgaaca
     1201 ctcccattcc ccccagcccc agcggaccgc tcccccgcaa cagccgctgc agcagcgttt
     1261 ccacctgatc atcgcggtga tgggccagca gcagcacatc gcccggctgg caatgctggt
     1321 ggaaggcggc gtaacgggcg gcacgggcgg attgctccag actggaccgg gtgcgatcga
     1381 tggcaacggc ctgcgccaca aacggcacct gccagtgttt gcacagttcg gcgcactgcc
     1441 actgccagtc gtcggcatga gggtgtagtt gatgattgat gtgaagagcg agcagcaggg
     1501 tggaagaatg atgatggcgc cattgccgcg ccagctgcag cagcacggtg gaatccaggc
     1561 cgccgctgaa accgaccacc agacgggcgg gtggaggctg ggcaggtggc cccggccttg
     1621 cggccagggc ctgatcgatc agggttgcat ccattgaaag gtcgcggtgg actcgctcag
     1681 atcatgcaat gcggcgcatc tgcttgcgcc ggtacaccgc aatcacgatc gccaacagtg
     1741 tgagcagcag cggtacccct gcaatattga tcagcttgag cctgaagtcc agccggtcga
     1801 tatcctggtt cagctggtgc tgcacgtcgc gcagctgctt gcggatctgc agcttttcct
     1861 gctggaactt gagcaggtcg gactgcattt ccggcgtcag ggtaatgaca ttgccatcct
     1921 cgccctgctt ctgctgcaac tccgccagct tgccttcgat ttcctgcagc cgcgccgtga
     1981 gggattcctg cacctgccgg aatgatgctt ctgcattgcg ctgcatgtcc tgcaccacgg
     2041 tgaacgggcg gaagaagcgg ccacgaccgc ggatgccgat caggtcggcg ctgccagaca
     2101 gcacatcgac gctgttgaag aagaagtcgc cgttgtcggc gaagggctgc gccacctgct
     2161 ggccgaagaa ctggtgcacc tgcacccaga gacggtcggt cagcatatcg gtgtccgcca
     2221 cgatcaccac gttgatcggc ttcagcgagg ctttcaggta ttcatcgtca gccttggccg
     2281 gtgtcttctc cgtcaccttg gcatccggct tgccctggcc agcctccgcc gcaccttccg
     2341 actgcgggac gccgtccggg aacgcgctct gcacatcgcc ggtcacccgc gcccccagca
     2401 cgaagggcgt gccgctgggg gtgaagccct gcgtcagttc gcggggatcc tgcatcattg
     2461 ccagcttctc ggcatcaaag gccatcgcgt gttcggagct gctcagcaag ggttcaaagg
     2521 tggttttggc gtccttggcg tgagcaacga aaccgacgct ggacatatgc agcgtctcca
     2581 gcttgctgac aatcatgttg tcgcgggcca gattgcggtc gctcaattgc aggattgcca
     2641 gatgccgcac cggcgcacgc ccctgcccca tgcccacctg caacgccagt tgcgcgtcgc
     2701 cgagcacttt cgtcttgtca tagtccacgc cccaggcctt gaacaaccgg ttcaggtccg
     2761 atgcgcgatc cgtcgaggtg agaccctttt cctgttcggc aaagggatcg acaaaaatca
     2821 gggcatggcc accgcgcagc acatactggt cgatggcgaa cagcatggcg tcggacaggt
     2881 ttttcggatg gatcaccagc agcagttgat aatcagtggt gctgatttcc tgctccgtgc
     2941 cagtaagttt ctgcacgtcg aacaactgct ccagctggga gatggtggcc cagggctgcg
     3001 acggctgccg catcatcatg tcgaaaccgc cctgcacttc cagatccgtc agcagcgcga
     3061 ctttcggctt ctgctgctgg ctgacctgat agaccagttt ggcaatgtca tattccagga
     3121 attcctgccg gtcgggctgg aacacgggaa tcacttcggt gcgcggttcg ggtttttctt
     3181 ccgctgtctt gccgtccccg tcctgattgg catcgccttc cgctttgggc ggttgcacct
     3241 gcgctaccag accgaaaaac actttctcct cgccaccgcc ggcattgatc gcctgcaggc
     3301 caaacgcggc agcacggtcc tcctcttccg agaacggctc cgggtcgatc acatgcagtt
     3361 tcagcttgcc gttggaggcc agggcaaact cctccagcag ctcacgcacc tgcttggcat
     3421 aggtgcgcca gccctgcgca tcgcgggttg cttcctggga gaagtagaac tgcagctcaa
     3481 ccggttgctg gatgtcgcgc acgatctgtt tgctgccgtc ggacaaggtg tacaggttgc
     3541 cttcggtgag atcgaagcgc acacccttga acagccagtc ggtgaacagg gtggagaaca
     3601 acaccaccag tgccagcagg atcagtccag cgccggaaaa ccatttattg actcgcatcg
     3661 cgtcggctcc ttctcaattc gcttttttca tgtcgatgac aatggcattc gcaatcagcc
     3721 agccaccgat caaaaacaca aaataaaaga catcacgcag atccagcacg cccttggaga
     3781 tggagttgaa atgggtgatg tagctcagcg cggccacggc ggtcgaaacc gattccggca
     3841 tccattgcgg cagccagccc tgcacgattt ccatgccgct gaccatgaac agaaagcaca
     3901 ccaccacgct gatgatgaac gcaataacct gatttttggt ggtggcggaa atgcaggagc
     3961 caatcgccag caggccgccg gccatcagcc agcttcccag atagctggcg acgatcacgc
     4021 cgttgtcggg atcgcccagg taattcacgg tgatccagat cggaaacgtc agcaccagcg
     4081 cgatgccggt gaatacccag gccgccagaa atttgcccag caccatgtca cgcagggtga
     4141 ttggcaatgt catcagcaat tccagcgtgc cgctcttgcg ttcctccgcc cacagccgca
     4201 tggacagcgc cggcaccagg aacagatata gccagggatg gtaggcaaaa aagccgcgca
     4261 gatccgcctg cccgccctga aagaaattgc cgaagaaaaa ggtgaaggaa cccgccagca
     4321 ccaggaagat aagaatgaag acgtacgcca gcggcgtagc gaaatagctg gccagttcgc
     4381 gtttcagaat cacggtcagg ttattcatca ggccacctca cgggtaatgt cgcggaacac
     4441 ttcatccagg cggccgcgct ccacatgcag ctcgcgcacc tgccactgat gttgctggat
     4501 ggcgttgttc accacttcaa acagttgctg tccggctttg gggaacacga tcagggtgtg
     4561 atcctcgcca gcctcgatgt gatccacctg cggcagcgcg gacaggatct gctgcgcgtt
     4621 gctggcgtgc tccaggccca gggtcacggc gccatggtaa cgggaacggg cttccagttg
     4681 cgccggggtg ccgtccgcca ggattttgcc gccggcaatg atgatcgcgc gggagcagac
     4741 ggcggacact tcttccagaa tgtgggtgga gacaatgaca atcttgtctt tcgacaggtt
     4801 ggcgatcagg ttgcgcactt cgtgcttctg gttcggatcg aggccatcgg tgggctcgtc
     4861 caggatcaat acctgcggat catggatgat ggcctgggcc aggccgaccc ggcgccggaa
     4921 acctttcgac agggtttcga tgggctggtt gagcaccttg tccagttgca gcgcgtgctg
     4981 gcagcgttcc acgcgggcgg ccagttcggc gccccggtaa ccgcgtacct ggccgatgaa
     5041 gcgcaggaac tgcagcactg tcatatcgcc ataactgggg gccccttccg gcaggtagcc
     5101 gatcagctgc ttcgccttca ccgggtggtg ggcaacatcg tggcccatga tggtcacctg
     5161 gcccgacgtc ggcgccagaa aaccggtgat catcttcatg gtggtggact tgccggcgcc
     5221 gttggggccc aggaatccca acacctcgcc cggtgctacc gtaaatgaga gtccgttcac
     5281 cgccgtgaac tggccaaact ttttggtcaa gttgtcgatt ctgatcatgg tggttccttt
     5341 cccgattacc caagcctggc gcaggccacc ggcaaacccc aaggtatgcc ccgccaggaa
     5401 tgttgtgaga aaaatttcgc cccgtttgac cgggttgcgg ctggatggtt cgccgtcagc
     5461 ggtcaatttt tcgaatgcgc actataaaaa ccggcccctg ccttttcaat aggccctcca
     5521 ccataacgat ttgttacaga ggtgtcgccc attgttttgc ctgctccctc ctttgcctgt
     5581 ataagccatt gggattagac ttcagcccac ttttgatccc cgggcccttt cacaacggcc
     5641 ggactcttta cgataggtag ttcaatgacg cgctggttag gactcgatct actctttata
     5701 tggctggtga ggaaatttct gttcctgatg gtacgcaccc aggtgcagcc cgatgacatc
     5761 agcaggctca atctcgaccc cgcccgcccg gtctgttacg tgctgaagaa tgaatcgctg
     5821 gcggattacc tggtcgccga ccaggaatgc cgcaacctgg gcctgccacg cccgtccgac
     5881 ggattcagcc tgggcacctt caaccagcgt catggcgtgt tctttctcac ggaacggggc
     5941 cacttctggc agcgcaaggc catgctgcag cgccagccga aaaaattgct ggccatcgtc
     6001 gaatggctgg agcagaaccc gcagcaggac atccagctgg tgccggtatc ggtattctgg
     6061 ggccgggcgc ccgaccgcga aaattccatt ttcaagatcc tgttctctga cagctggggc
     6121 gtacccggct tcttccgcaa gctgttcatc attttcgccc agggccgtca aaccctggtg
     6181 tatttctccg aaccggtttc ggtacgcacc gtgctcaccg aagggctggc aaaggaacgc
     6241 acggcccgca agatctcgcg cgtgttgcgc acccacttcc gccgcctgcg ggaaaccgtc
     6301 attggacctg acctgtcaca tcgccgcacc ctggtgaatg caatcctgcg catgcccagc
     6361 gtggccgccg aaatcgaagc agaaacccgg cgcaacaagc tcacctacaa gaaagcattc
     6421 gacaacgcgc gcagctacgc cgaagaaatt gcagcagact attcccacgc cgtgatccgc
     6481 ctctacctgc tggtgctgac ctggctgtgg aaccggctgt atgacggtgt acaggtacat
     6541 ggcctcgatc gtctgtacaa ggtggcgaag gataacgaga tcatctacgt gccctgccat
     6601 cgcagccaca ttgattatct gctgatgtcg tatgtgctgt tcgtgaacgg actggtacca
     6661 ccgcacatcg ctgccggcgt caacctgaac atgccggtgg taggcccgat cc
//
LOCUS       DASUEG010000435         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_946463, whole genome shotgun sequence.
ACCESSION   DASUEG010000435 DASUEG010000000
VERSION     DASUEG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946463"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFX11_08150"
     CDS             complement(<1..297)
                     /locus_tag="VFX11_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX4938629.1"
                     /translation="MKNKDLQVIIAQLDFLVGDIDGNTDLILQAAQRARAKFQADLIV
                     FPELTLTGYPPEDLLLRPSIQVRVEKALHRLRSELPRDLYVLVGYPRRLDGGALF"
     gene            421..663
                     /locus_tag="VFX11_08155"
     CDS             421..663
                     /locus_tag="VFX11_08155"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX4938630.1"
                     /translation="MLTRIIVVAVLITAAWFLYRKYVAAKDIKIPGANKRQAIMKKCA
                     HCGIHLPEAESVQLEGLHFCSEAHKLEYQKQHTPHE"
     gene            656..2296
                     /locus_tag="VFX11_08160"
     CDS             656..2296
                     /locus_tag="VFX11_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4938631.1"
                     /translation="MNEATTPAGNLHTTALPIAHQNEWRLLQVYLYYRLLLSLSLLAA
                     LFFSDNKQSEWLSDDQQLYFATAATYLVLTVLSLLTARLRTGRLSIQVFIIIVTDILA
                     IALLEYANGQSNSNLTILLVVTVAAGSILVEGKLANFFAAIATLGVLYREIYTMVIKG
                     AFLQQELLQSAMIGIACFATSILAQQIAKRLRESAELAGKQAADLANLEELNHHIIQR
                     MRTGILVVDGKNQIVLVNDACWKLFGMPTLSDHDNLENISPELAQQLGQWRSNPFARS
                     RPFRAGLSGPEVQANFTSLETGGSPTALIFIDDNTRMAQQAQQLKLASLGGLTASIAH
                     EIRNPLGAISHAAQLLQESTSLNKGDARLAEIIHQHCVRMNKVIENVLQLSRRKPVEP
                     ELFNMKDWLENFIADFKSTQNEAVDIEFKITRPDLTFRIDPSQIGQVLTNLFQNGLRY
                     SEKNTGRRFLRVIADFSIQTEQPTLDIIDRGPGVSEDMRDKIFEPFYTSEKSGTGLGL
                     YIARELCEANQARLDYIPTRSGSCFRITFSHPARIVTI"
     gene            2298..3674
                     /locus_tag="VFX11_08165"
     CDS             2298..3674
                     /locus_tag="VFX11_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4938632.1"
                     /translation="MSTYKALIVDDEPDIRELLELTLSRMDVEVFTAANVESAHVSLQ
                     QEKFDLCITDMNLPDGNGIDLVKYIQANYPHVPVAVLTAYGSMETAITALKAGAFDFV
                     SKPVELQRLRDLVNTALKLKAQKKETIAETPETPILGLSPCVKKLHAQIAKLARSQAP
                     VYISGESGSGKELVAREIHRLGPRADGPFVPVNCGAIPSELMESEFFGHKKGSFTGAY
                     EDKTGLFQAAQGGTLFLDEVADLPMQMQVKLLRAIQEKAVRPVGSQREITTDVRILSA
                     THKDLAKLVEQGDFRQDLFYRINVIQVSVPPLRERKEDIAFLAQHFLQVLANDWQMDL
                     PRLSPNALKALENYQFPGNVRELENILERAVTLCDDDVIAPEHLQLPTDGVPVARVNP
                     AVGDRNLPDFEHGDSLEDYLTDIEKQAIMRALEETRWNRTAAAKKLGMSFRSLRYRLK
                     KLGLDTDD"
     gene            complement(3688..>3954)
                     /locus_tag="VFX11_08170"
     CDS             complement(3688..>3954)
                     /locus_tag="VFX11_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4938633.1"
                     /translation="FDMVPVLRDVPVVKQWAGLRPGSPGGLPFIGRIPGWNNLHVNAG
                     HYRNGLVLAPASARLMADLLLQRAPVVDPAPYDPAGIRAVMEMV"
BASE COUNT          915 a         1218 c         1106 g          715 t
ORIGIN      
        1 gaacagcgca ccgccgtcga gacgacgggg gtaaccaacc agcacataca ggtcgcgcgg
       61 caattcagaa cggagcctgt gcagcgcttt ctcgacccgc acctgaatac tggggcgcag
      121 cagcaggtct tcaggggggt agccggtcag ggtaagctcg ggaaaaacaa taagatcagc
      181 ctgaaatttg gcacgggcac gctgcgccgc ctgcaggatc agatcagtgt tgccatcgat
      241 gtcgccaacc agaaaatcca gttgcgctat aatgacttgt aaatccttgt ttttcatgca
      301 gtttagatcc tgttaacagc aatgcagccc agactgtgaa aaatgggcgt attgtggggc
      361 aaatcgccgg aactggcaaa gccccggaca ttcggaatcg cacgacggaa tgaacaactt
      421 atgctgaccc gtattatcgt tgtggcagtg ctgattacag ctgcctggtt tctctaccgc
      481 aagtatgtcg ccgcgaaaga catcaagatt cccggtgcca acaaacggca agccatcatg
      541 aaaaaatgcg cgcactgcgg cattcatctg cccgaggcag aatcagtgca actggaaggt
      601 ctgcacttct gcagtgaagc acacaaactt gaataccaga aacagcacac gccacatgaa
      661 tgaggcaacc acccccgccg gcaatctcca caccaccgcg ctgccgattg cgcaccagaa
      721 cgagtggcgg ctgctgcagg tgtacctgta ctaccggctg ctgctgtcgc tgagcctgct
      781 ggcagcgctg tttttcagcg acaacaagca gtcggaatgg ctgtctgacg accagcaact
      841 ctatttcgcc accgccgcca cctatctggt actgacggta ttgtcgctgc tcaccgcccg
      901 tttgcgcacc ggccgcctga gcatccaggt gttcatcatc atcgtcaccg acattctcgc
      961 catcgcattg ctggaatatg ccaatggcca gtcgaacagc aacctcacca tcctgctggt
     1021 ggtgacggtg gcggccggca gtattctggt ggaagggaag ctggcgaatt ttttcgcggc
     1081 cattgccacc ctgggcgtgc tgtaccgcga aatctatacc atggtgataa agggcgcgtt
     1141 cctgcagcag gagctgttgc aaagcgcgat gatcggcatc gcctgcttcg ccacgtcgat
     1201 cctggcgcag cagatcgcca agcgcctgcg ggaaagtgcg gagctggcag gcaagcaggc
     1261 ggcggacctg gccaatctgg aagaactcaa tcaccacatc atccagcgca tgcgcaccgg
     1321 cattctggtg gtggatggca aaaaccagat cgtgctggtg aacgacgcct gctggaaact
     1381 gttcggcatg cccaccctgt cggaccatga caatctggaa aacatctcgc cggaactggc
     1441 gcaacaactg ggtcagtggc gctcgaatcc gtttgcccgc tcgcgcccgt ttcgcgccgg
     1501 actgtcgggc ccggaagtgc aggcaaattt cacgtcactg gaaaccggcg gcagccccac
     1561 cgcgctgatc ttcatcgacg acaacacccg catggcgcag caggcacagc agttgaaact
     1621 ggcgtcactc ggcggcctca ccgccagcat tgcccacgaa atacggaacc cgctgggtgc
     1681 catcagccat gccgcacaac tgctgcagga atccaccagc ctgaacaagg gcgacgcacg
     1741 gctggcggaa atcatccacc aacattgcgt gcgcatgaac aaggtgatcg agaatgtgct
     1801 gcagctgtcg cggcgcaaac cggtggagcc ggagctgttc aacatgaagg actggctgga
     1861 gaatttcatc gccgacttca agtccaccca gaacgaggcc gtggatatcg aattcaagat
     1921 cacccgaccg gacctcacct tccgcattga cccgtcccag atcggccagg tgcttaccaa
     1981 cctgttccag aacggcctgc gctacagcga gaaaaatacc ggacggcgtt tcctgcgcgt
     2041 catcgccgat ttcagtattc agacggaaca gcctacactg gatatcatcg accgcggccc
     2101 cggcgtttcc gaggatatgc gcgacaagat tttcgaacct ttttacacct ctgaaaaaag
     2161 cggtaccgga ctggggcttt atatcgcacg ggaactgtgc gaggccaacc aggcgcggct
     2221 ggactacatt cccacccgca gtggcagctg tttcagaatc accttttccc atccggccag
     2281 aatagtgacc atttgacatg agtacctaca aagccctgat cgttgacgac gaacccgata
     2341 ttcgcgaact gctggaactc accctgtccc gcatggatgt cgaggtcttc accgccgcca
     2401 atgtggaatc cgcccatgtc agcctgcagc aggagaagtt cgacctctgc atcactgaca
     2461 tgaatctgcc ggacggcaac ggcatcgatc tggtgaaata catccaggcc aactaccccc
     2521 acgtaccggt ggccgtactg accgcctacg gcagcatgga gacggcgatc accgcactga
     2581 aggcgggggc ctttgatttc gtgtccaagc cggtggaact gcagcgactg cgggatctgg
     2641 tgaacaccgc gctgaaactg aaagcgcaga aaaaggaaac cattgcggaa acgccggaga
     2701 ccccaattct gggcctctcg ccctgcgtga aaaaactgca cgcgcaaatc gccaagctgg
     2761 cccgcagcca ggcgccggtc tacatcagcg gcgaatccgg cagcggcaag gagctggtgg
     2821 ctcgggaaat ccaccgtctc ggcccccgcg ccgatggccc ctttgtgccg gtgaactgcg
     2881 gcgccattcc cagtgaactg atggaaagcg aattcttcgg ccacaagaaa ggcagtttca
     2941 ccggcgctta tgaagacaag accggtctgt tccaggccgc ccagggcggc acgctgtttc
     3001 tcgacgaagt ggctgatctg cccatgcaga tgcaggtgaa actgttgcgc gcgatccagg
     3061 aaaaagcggt gcgcccggta ggttcccagc gcgaaatcac caccgacgtg cgcatcctgt
     3121 ccgccaccca caaggatctg gccaagctgg tggagcaagg ggattttcgc caggatctgt
     3181 tttatcgcat caacgtgatc caggtgagcg tgccaccact gcgggagcgc aaggaagaca
     3241 ttgcgtttct ggcccagcac tttttacagg tgctcgcaaa cgactggcag atggatctgc
     3301 cgcgtctgag cccgaacgcg ttgaaggcgc tggaaaacta ccagttcccc ggcaacgtgc
     3361 gcgaactgga aaacattctg gagcgcgccg tcaccctgtg cgatgacgat gtgattgcac
     3421 cggaacatct gcaattgccc accgacggcg tgccggtggc gcgcgtcaat cccgccgtgg
     3481 gcgaccgcaa cctgccggac tttgagcacg gcgattcgct ggaagattat ctgaccgaca
     3541 ttgaaaagca ggcaatcatg cgtgcactgg aagaaacccg ctggaaccgc accgccgccg
     3601 cgaaaaagct cggcatgagt ttccgctcgc tgcgctaccg gctgaaaaaa ctgggactgg
     3661 atacagacga ctgagcctgt cgtccggtta caccatttcc atcactgcgc ggatacccgc
     3721 cggatcatac ggcgccggat caaccaccgg cgcgcgctgc agcaacaagt ccgccatcag
     3781 tcgcgccgat gccggcgcca gcaccagtcc gttgcggtag tgccctgcat tcacatgcaa
     3841 attgttccaa ccgggaatcc ggccaataaa aggcaggcca ccgggcgagc ccgggcgcag
     3901 gccagcccac tgtttcacca ccggcacatc ccgcaacacc ggcaccatgt cgaa
//
LOCUS       DASUEG010000436         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_649689, whole genome shotgun sequence.
ACCESSION   DASUEG010000436 DASUEG010000000
VERSION     DASUEG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649689"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1013
                     /locus_tag="VFX11_08175"
     CDS             123..1013
                     /locus_tag="VFX11_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4938634.1"
                     /translation="MDKLRCMEVMVAVAEAGSFTAAADRLEISAVMVGKHIRQLEAHL
                     GARLLQRSTRKQSLTDVGVAYYDDCKRVLEQVRWAESAVERSRAAPQGLLRVSAPVTL
                     GSQVIAPLVARYLQQHEKVRVDLQLTDSVVDLAGEAIDAAIRIGHVTDENLVARPLRP
                     YRMVIAAAPSYLKKRGTPRKAADLAHHDCLSHSVWQRRIEWTLQDGNDTLLWPEQARL
                     VCNQGDGLRTAALQGLGLVMQPEVLLADDLASGRLRQVLPHCLPPARPVHLVYGADRR
                     QLPKLSHFIEQVVKALGTAA"
     gene            complement(1064..2239)
                     /locus_tag="VFX11_08180"
     CDS             complement(1064..2239)
                     /locus_tag="VFX11_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4938635.1"
                     /translation="MNAVTKHEYDRIPYMIVFQEKASFKDTYAGRMDFVGLEAHLLKP
                     KDVDSKTVIVFMHPIGGGAYLPMVSSLAKKGFHVIYCNSRYRGVDSALAMEKVALDLG
                     ACIRDAKERLGYEKVVLAGWSGGGSLSLFYQSQAEHPTITHTPAGDPVNLVDAKLIPA
                     DAMMLLAAHISRAGTLTEWLDASILDESDPTKRDRELDIYDSNNPNQPPYSAAFVEKY
                     RAAQIARNRRITQWVKDKLAAFKRDGLENEEFAFVTHGTMADPRFVDPTLDPNDRKPN
                     WCFLGDPRVVNNGPVGIARFNTLRSWLSQWSYDDSNADGLKCAQKISVPTLVIGNTAD
                     DACTPSHTHRLFEAVAVKDKELYEVKGANHYYFGQPKEMEEAVSQCAQWLTKKGFAA"
     gene            complement(2266..2898)
                     /locus_tag="VFX11_08185"
     CDS             complement(2266..2898)
                     /locus_tag="VFX11_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4938636.1"
                     /translation="MTETAPSADTRPPVVLKPVHNMHHTAYRCRDAEQTRWFYEDVLG
                     FKLIAALAFDEEPGRAVQREFMHLFFETTDGNCIAFFDAPDDADESMFVARHGFDQHI
                     AFEVENMEQLKAWRRHINKMGRPCFGPINHHFVHSIYMYDPNGLQVELTVKDADYQGI
                     MTRETAKAHADIQQWTERTRAIKEARLDKNMLDLRGIDTSKVDLSGMKKI"
     gene            3053..4252
                     /locus_tag="VFX11_08190"
     CDS             3053..4252
                     /locus_tag="VFX11_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4938637.1"
                     /translation="MTPLHDLTILDLTHMLSGPYASQLLADMGARCIKIEPPQTGEGT
                     RKLLADDPRYSIDGMGAYFLTLSRNKESVCLNLKEKAGLDLFYELVKKADVVINNFGE
                     GVPKRLGIDFDRLKQHNPRIITCSITGFGETGPERDRPAFDLVAQGMGGGMSITGEEN
                     GPPLRAGIPIGDLGGGLFATIGILSALHARTLTGRGQHVDISMQDCQVSLLNYMATMY
                     LMSGVQPPAVGNGHFVHVPYGTFRTQSRYIILACMGDAFYLKLAAMLNDPFLLEKRFE
                     KQPERWEHRHAINAKVQEYLEKDTCEFWLEQLKEYRIPAAPVNDFAFALRDPQIQARD
                     MVVDVPLPNGNTVPQPGNPVKLSDTNEQHYAAPPLLGQNTDAVLAELLGLTEARIGQL
                     RQAGIIG"
     gene            4464..>4693
                     /locus_tag="VFX11_08195"
     CDS             4464..>4693
                     /locus_tag="VFX11_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX4938638.1"
                     /translation="MRDIIYINEVGLRDGLQNQPNPVSTESKLKMAAALVAAGVRQFE
                     AVSFVHPKLVPQMADAVEVLAGLREFNQLNIAA"
BASE COUNT          924 a         1363 c         1409 g          997 t
ORIGIN      
        1 acaagcagaa ttttcatgag cgttttcctt gtgtgattga acgtgagtcc attgtgttga
       61 taaccaattg ggggataaag tagcgatcga ttcgctcatt cctaaccatt ggtttttaat
      121 tcatggacaa gctgcgctgt atggaagtca tggttgcggt ggccgaggcg ggcagtttca
      181 ccgctgccgc cgaccggctg gagatctcgg cggtgatggt gggcaaacat atccggcagc
      241 tggaggcaca tctgggggcg cggttgctgc aacgcagcac gcgcaaacag agcctgaccg
      301 atgtgggcgt cgcctattac gacgattgca aacgggtact ggaacaggtg cgctgggcag
      361 aatcagcggt ggaacgcagc cgggcggcac cccagggcct gctgcgggtg agcgctcccg
      421 tcaccctggg ctctcaggtg attgcgccgc tggttgcccg ctatctgcag cagcatgaaa
      481 aagtgcgcgt ggatctgcag ctgaccgaca gcgtggtcga tctggcgggg gaggcgatcg
      541 atgctgcgat tcgcatcggc catgtaacgg acgaaaatct ggtggcaaga ccgctgcggc
      601 cttatcgcat ggtgattgcc gccgcgcctt cctacctgaa aaaacgcggc acgccacgca
      661 aggctgcaga tcttgcccat cacgattgtc tgagccattc cgtgtggcag cgccgcatcg
      721 aatggacatt gcaggacggc aacgacaccc tgctgtggcc ggagcaagcc cgtcttgtat
      781 gcaatcaggg cgacgggctg cgcactgccg cgttacaggg gttgggtctg gtgatgcagc
      841 cggaggtgct gctggcagac gatctggcca gtggtcgtct gcggcaggtg ctgccacact
      901 gtctgccgcc ggcacgcccg gtacatctgg tgtatggcgc cgaccgtcgc cagttaccca
      961 agctgagcca ctttatcgaa caggtggtga aggcactggg aacggctgcg taacgctgcc
     1021 tttaccggct caccaaccca ccttttcgct gatatgacga ccctcaggcg gcgaaacctt
     1081 tcttcgtcag ccactgcgcg cactggctta ccgcttcttc catttccttg ggctggccga
     1141 agtagtagtg attggcgcct tttacctcat acagttcctt gtccttcacc gcaacggctt
     1201 cgaacaggcg atgagtgtga ctcggcgtgc aggcgtcatc ggcggtgttg ccgatcacca
     1261 acgtcggcac ggaaattttc tgtgcgcact tcaggccatc ggcgttggaa tcgtcatagc
     1321 tccactgcga cagccagctg cgcagagtat tgaaacgggc aatccccacc ggaccgttat
     1381 tcaccacccg cggatcgccg agaaaacacc agttgggctt gcgatcgttc gggtccagcg
     1441 tcggatcgac gaagcgcgga tcggccatgg tgccgtgagt cacaaacgca aattcctcgt
     1501 tctccagtcc atcgcgcttg aatgcggcca gtttgtcctt cacccattgg gtgatgcgac
     1561 ggttgcgggc aatctgcgcg gcgcggtact tctccacgaa cgcggcggaa tagggcggct
     1621 ggttgggatt gttgctgtcg taaatatcca gctcgcggtc acgtttggtg ggatcggatt
     1681 catccaggat cgacgcgtcc agccactcgg tcagcgtgcc ggcgcgggaa atatgcgcgg
     1741 ccagcagcat catggcatcg gcaggaatca gtttcgcgtc caccagattc accggatcac
     1801 cggccggtgt gtgcgtgatg gtgggatgtt cggcctggct ttgatagaac aggctcaggg
     1861 aaccgccgcc gctccagccg gccagcacga ctttttcata acccagccgc tcttttgcat
     1921 cgcggatgca ggcgcccaga tccagcgcca ctttttccat ggccagcgca ctgtccacgc
     1981 cgcggtagcg actgttgcaa taaatcacgt ggaacccttt tttcgccagc gatgacacca
     2041 tcggcagata ggcgccgcca ccaatcgggt gcatgaaaac gatgaccgtc ttgctgtcga
     2101 catctttcgg tttcagcaga tgcgcttcca ggccaacgaa atccatgcgg ccggcgtagg
     2161 tgtccttgaa gctggctttt tcctggaaca cgatcatgta gggaatgcgg tcgtattcgt
     2221 gtttggtcac tgcgttcatg tattcggttc cccggtaaaa aagtgtcaga tttttttcat
     2281 tccgctcaga tcgaccttgc tggtatcgat gccgcgcagg tcgagcatgt ttttgtccag
     2341 gcgtgcttcc ttgatggcgc gggtgcgctc ggtccactgc tggatgtcag cgtgggcctt
     2401 ggcggtttcc cgggtcatga tgccctggta atccgcatcc ttcaccgtga gctccacctg
     2461 caggccattg ggatcgtaca tgtagatgga gtgcacgaag tggtgattga tggggccgaa
     2521 gcaggggcgg cccattttgt tgatgtggcg gcgccaggcc ttgagctgtt ccatgttctc
     2581 cacttcaaac gcgatgtgct ggtcgaatcc gtggcgggcg acgaacatgc tttcgtccgc
     2641 gtcgtcgggc gcgtcaaaaa atgcgatgca attgccgtcc gtggtttcga aaaacagatg
     2701 catgaattcc cgctgcacgg cgcggccagg ttcctcgtcg aatgccagcg cggcaatcag
     2761 cttgaaaccc agcacgtctt catagaacca gcgcgtctgc tctgcatcgc ggcagcggta
     2821 ggcggtgtga tgcatgttgt gcaccggctt caatacgaca gggggacggg tgtcggcaga
     2881 tggggcggtt tcggtcatgg cgattccctc ggcggtgcca ggcaagattg gggttgactg
     2941 atgggtcgag tttttcatag attgtataca atttcaagta acaggcgggt ccttataacg
     3001 cttttcgcgg attggccgcc aattatgtat acataaattc accgaggccc tcatgacccc
     3061 gctgcacgac ctcacgattc tggacctgac ccacatgctg tccggcccct atgccagcca
     3121 gttgctggcg gacatgggcg cccgctgcat caagatcgaa ccgccccaaa ccggcgaggg
     3181 cacccgcaag ctgctggcgg acgatccgcg ctattccatc gacggcatgg gggcctattt
     3241 cctcaccctg tcgcgcaaca aggaaagtgt gtgcctgaat ctgaaggaaa aagcggggct
     3301 ggacctgttt tacgaactgg tgaagaaagc cgacgtggtg atcaacaatt tcggcgaggg
     3361 cgtgccgaaa cgcctgggca tcgatttcga caggttgaaa cagcacaacc cgcgcatcat
     3421 cacctgctcg atcaccggct ttggtgaaac cgggccggag cgcgaccgac ccgcgttcga
     3481 tctggtggca cagggcatgg gcggcggcat gtcgatcacc ggcgaggaaa acggtccgcc
     3541 gctgcgggcg gggattccca tcggcgatct gggtggcggc ctgttcgcga ccatcggaat
     3601 attgtccgca ttgcacgccc gcacgctcac cggacgcggc cagcacgtgg atatttccat
     3661 gcaggattgc caggtgtcgc tgctgaatta catggccacc atgtacctga tgagcggcgt
     3721 gcaaccaccg gcggtgggca acggccattt cgtgcatgtg ccctacggga ctttccgcac
     3781 gcaatcgcgc tacatcatcc tggcctgcat gggcgatgcc ttttacctca agctggcggc
     3841 catgctgaat gatccgtttc tgctggaaaa acgctttgaa aaacaacccg agcgctggga
     3901 acaccgccat gccatcaatg ccaaagtgca ggagtatctg gaaaaagaca cctgtgaatt
     3961 ctggctggag caattgaagg aataccgcat tcccgccgcg ccggtgaatg atttcgcgtt
     4021 tgccttgcgt gatccgcaaa tccaggcgcg ggacatggtg gtggacgtcc cgctgcccaa
     4081 cggcaacacc gtgccgcaac cgggcaatcc ggtgaagctg tcggatacaa acgaacaaca
     4141 ttacgcggcg ccgccattgc tgggccagaa cacggatgcg gtgctggcgg agctgctggg
     4201 tctgacggag gcgcggatcg ggcagctgcg tcaggcggga atcatcggat aatcgcgtcc
     4261 cgaacgattt cccccgtatc cggccacaac agacaaatcc gcttttttga gcctgccggt
     4321 ggccccctgc caccggtctt ttcgagcaca ttccggaact tcttccatta cctgccggca
     4381 cagcaaacgc tggcaaggta gcgccacacc cgctttcgct tataatcgac ccaatttcct
     4441 tgactcatcg gtccagacat cccatgcgcg acatcattta cataaacgaa gtaggcctgc
     4501 gcgacgggct gcaaaaccag cccaatcccg tttccaccga atccaaactc aagatggcgg
     4561 ccgccctggt ggccgccggc gtgcgccagt tcgaagccgt cagtttcgtg catcccaagc
     4621 tggtgccgca gatggcggat gccgttgaag tactggcggg cctgcgcgaa ttcaaccaat
     4681 tgaacattgc cgc
//
LOCUS       DASUEG010000437         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_674249, whole genome shotgun sequence.
ACCESSION   DASUEG010000437 DASUEG010000000
VERSION     DASUEG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674249"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFX11_08200"
     CDS             <1..911
                     /locus_tag="VFX11_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX4938639.1"
                     /translation="DCLPVYCFEMGSTQPSAPVIIFSGGVHGVERIGTQVLLSFMHTL
                     FARLEWDESLDDLLQQVRLVFVPLVNPGGMLIRHRANPNGVDLMRNAPIDAQGNVPFM
                     LGGHRVSRYLPWYRGRRNAGMEPEAQALLNLVESCVLESDFVISLDCHSGFGHQDQVW
                     FPYAFTPDPFDDIGEMFALRLLFRRTHPNFSFYKIEPQSRNYTTHGDLWDHVYRKTLH
                     RRKGVYLPLTLEMGSWLWVKKNPLQIFNSMGIFNPVLPHRHERVLRRHQCLMEFLIRA
                     ARSFRNWLPHPADRRDWMHQAIEHWY"
     gene            complement(841..1689)
                     /locus_tag="VFX11_08205"
     CDS             complement(841..1689)
                     /locus_tag="VFX11_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4938640.1"
                     /translation="MILRSIKAVRLFNHILLGLVLLVLTGAFLNNGTRLLKSTKQWWL
                     GRITRLLGMEIEVIGEFPRGTGDAGLLLVSNHVSWTDIPVIGSLRQINFLSKAEVRKW
                     PLVGRLAQTTGTLFIQRGKGDTDSVARQMADYLAQGRSVLFFPEGTTSDGRSVNRFHR
                     KLFRTCEHVPTQVLPLAIHYEVEGLDHTAVARNPVAFIGDDEFTQHIWQLLAFSRVKV
                     KVAVLPPRMLNPATLEQDVTAIRQDILAQVHNFNALQPAAAFSTSARLPDASSRAGQP
                     DEAASF"
     gene            complement(1686..2480)
                     /locus_tag="VFX11_08210"
     CDS             complement(1686..2480)
                     /locus_tag="VFX11_08210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4938641.1"
                     /translation="MLQNDQACSGNTHTLPASRPRIIADFITTEDEIRRIQRLRYELF
                     CEEYNVTLPVNRMWNGLPIDADELDDHCLHLVVRDTNTGEPVGYTRVLTSAQAAVLGH
                     FYSSHEFHIDRIVNRPGRFLEIGRTCIHPDYRNGGTIAVLWAFLAKYMQEHDYQYLFG
                     CASISLNDGGTAYANIMQVVRDQFMAAEEFRVEPLLPLQLKDVSVEGKASFPPLLKAY
                     TRMGARICGEACWDPDFNVADVFVLLDVNNVAARYAKHFLKTQEAA"
     gene            complement(2700..>2854)
                     /locus_tag="VFX11_08215"
     CDS             complement(2700..>2854)
                     /locus_tag="VFX11_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX4938642.1"
                     /translation="VPNEGVKIFKNNELKNTVAGQEFKQALFGIWLSDKPAQKSLKDE
                     MLGSKS"
BASE COUNT          570 a          822 c          839 g          623 t
ORIGIN      
        1 acgactgtct gccggtctac tgttttgaaa tgggcagcac gcaaccctcg gcaccggtca
       61 tcatattttc cggcggtgtg cacggtgtgg aacgcattgg cacccaggtg ctgttgtcgt
      121 tcatgcacac cctgttcgcg cggctggagt gggacgaaag cctcgacgat ctgctgcagc
      181 aggtgcgcct ggtgtttgtg ccgctggtca atcccggcgg aatgctgatc cgccaccgcg
      241 ccaatcccaa cggcgtcgat ctcatgcgca acgctcccat cgacgcccag ggcaatgtgc
      301 cattcatgct gggtggtcat cgcgtcagcc gctatctgcc ctggtaccgg ggccgccgca
      361 atgccggcat ggagccggaa gcacaggccc tgttgaatct ggtggaaagc tgcgtgctgg
      421 aatcggattt cgttatttca ctggactgcc attccggctt cggccatcag gaccaggtct
      481 ggtttcccta cgccttcaca cccgatcctt tcgacgacat cggtgaaatg ttcgcgctgc
      541 ggctgctgtt ccgccgcacc catcccaact tcagcttcta caaaatcgaa ccgcaatccc
      601 gcaattacac cacccacggc gatctgtggg atcacgttta ccgcaagacg ctgcatcggc
      661 gcaaaggcgt ctacctgccg ctgacgctgg agatgggaag ctggctgtgg gtaaagaaga
      721 atccgctgca gattttcaat tcgatgggga ttttcaatcc ggtgctgccg catcgtcatg
      781 agcgtgtgtt acggcggcat cagtgtctga tggaatttct gatccgggcg gcacgcagtt
      841 tcagaaactg gctgcctcat ccggctgacc ggcgcgactg gatgcatcag gcaatcgagc
      901 actggtactg aatgcggcgg cgggttgcag ggcgttgaaa ttgtgcacct gtgccagaat
      961 gtcctgccgg attgccgtca cgtcctgctc cagcgttgct ggattgagca tgcggggcgg
     1021 taatacggcc actttcacct tcacccgtga aaacgccagc agctgccaga tgtgttgcgt
     1081 gaattcatcg tcgccgataa aggccaccgg attgcgcgca acggcagtgt gatccagccc
     1141 ttccacttca tagtggattg ccagcggcaa tacctgcgtc ggcacgtgct cgcaggtgcg
     1201 aaacagtttg cggtgaaaac gattcacgct gcggccatcg ctggtggtgc cttcaggaaa
     1261 aaacagcacg gagcggccct gtgccagata gtcggccatc tgccgggcga cggaatccgt
     1321 atcgcccttg ccgcgctgga taaacagggt gccggtggtc tgggccagcc gccccaccaa
     1381 cggccacttt cttacttcgg ccttcgacag aaagttgatc tgccgcaggc tgccgatgac
     1441 gggaatatca gtccaggaca catggttgct caccagcagc agtccggcgt cacctgtgcc
     1501 tcgcggaaat tcaccgatga cttcgatttc catccccagc agccgcgtga tgcggcccag
     1561 ccaccactgc ttggtgctct tgagcaggcg tgtgccattg ttcaggaacg cgcctgtcag
     1621 taccagcaat accagaccca gcaggatgtg attgaacagc cggaccgcct tgatcgagcg
     1681 taggatcatg ccgcttcctg agtcttcaga aagtgtttgg cataacgcgc ggccacatta
     1741 ttgacatcca gcagcacaaa cacgtccgcc acattgaagt ccgggtccca gcaggcttcg
     1801 ccgcagatgc gcgcacccat gcgggtatag gctttcagca gcggcggaaa gctggccttg
     1861 ccttccacgc tcacgtcttt cagttgcagc ggcagcagcg gttccacgcg gaattcctcg
     1921 gccgccatga actggtcgcg caccacctgc atgatgttgg cgtatgcggt gccgccgtcg
     1981 ttcagcgaaa tgctggcgca gccgaacagg tactggtaat cgtgctcctg catgtatttc
     2041 gccagaaacg cccacagcac ggcgatggtg ccgccattgc ggtaatccgg atgaatgcag
     2101 gtgcgaccga tttccagaaa acgcccgggg cggttcacga tgcgatcgat atggaattca
     2161 tggctggaat agaagtggcc cagcactgcg gcctgagcgc tggtcagcac acgggtgtag
     2221 ccgacgggct cgccggtatt ggtatcgcgc accaccagat gcaggcagtg atcgtccagt
     2281 tcatcggcgt cgatgggcaa accgttccac atccggttta ccggcagggt gacattgtac
     2341 tcttcacaaa acagctcgta gcgcaggcgc tgaatcctgc gaatctcgtc ttcagtagtg
     2401 atgaagtccg cgatgatgcg gggacggctg gcgggaagag tgtgtgtgtt gccggaacaa
     2461 gcctggtcgt tctgaagcat cgttgcctcc gcgagattgt gaaagctcga tgctgacttt
     2521 atggaaatgc tgttgcgggg aagttaaaga acgatgaaac ctttatgaca ctgtcacaaa
     2581 agtgttgtcg tcatggttgg gctgaaaaga tgctaaggga gggggagcgg caggttgtat
     2641 tcactatgag gaaaaagagc cggacagtgc cggctcccgg attacctggc tgcaatcgat
     2701 cagctcttgc tgcccagcat ctcatccttc aggcttttct gggcgggttt gtccgacagc
     2761 cagatgccaa acagtgcctg cttgaattcc tggcctgcca cagtgttttt cagctcgttg
     2821 ttcttgaaga ttttcacgcc ctcgttgggc acgt
//
LOCUS       DASUEG010000438         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_891284, whole genome shotgun sequence.
ACCESSION   DASUEG010000438 DASUEG010000000
VERSION     DASUEG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891284"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFX11_08220"
     CDS             <1..1176
                     /locus_tag="VFX11_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938643.1"
                     /translation="NFSNGECGNTFGAGMLMASAAAVSDLPYPLYLTDAYVQTSMFDF
                     NGDGVGDILYTMEVPVIGEENSENDWLISYGGMSEPELLQSEEEFVSQLVFGDVDGNG
                     SSEIVVERQDEAGYQWLATSGERKWVNVLPASVRFVSAGAAGSQIEVSHGGTKTVTTL
                     LDLNFGNFRGGPSYEALLTTQGSDAVTRFFMCELPSTACEQVVITGFVAQPDSLYIGQ
                     FDGDAYHEIFVSHDDDWYLVQPQQSGGWVTTLYRANTMNDLKDADSVGGQGYDVNQFT
                     SNISKSLRFGDFDGDGLTDVFTSRTKNDLGQWKWTVSYRGGDWEYLNAAWEHPSMLRV
                     SDFDGDAVADVFTASETTRDGLVDWSVCYRGKSTWVPLNANKNLPLSVLSLGETKFD"
     gene            1225..2925
                     /locus_tag="VFX11_08225"
     CDS             1225..2925
                     /locus_tag="VFX11_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938644.1"
                     /translation="MRLERVTIFNVPAAILALLATLFASVAPEAQAYTYDEAAAKKIL
                     YGLTGTGGRAFDTTKAVNSYAVFPETMKEADILAIVKNALEDDEDWEESEMDRRRRGK
                     TVEVDNLSVYIEVQHKPEKILDFYPVGGYWKSTDTVWANYAKSLPSMKIGVARIYHCS
                     DFCPSMLEGWHTDKGTLINTDNVSKKARKSSDLTFHIYDVGAGNCIIAECPAAGGENN
                     AIILDCGVSKAQLSSIVKPSATFGGKAPLMLEDMYDILAKKDKIDLILTHPDIDHYNL
                     IPYLFYSKKKTETENAIAPKLRSIYVGGLWTDYKDSALSVEAGAPAPKRRKTGGAKVH
                     EVLGKISDGTILASKPAVHAMINANDVYKNSSFTARSFKGNENLACGDAAKILAYNAY
                     PNRIGKEKGEESNGQSIIVAFQHGGKRIALTGDATDVGLRNAITNDATTGYLNGVDVL
                     VVPHHGSRLPTHFPAEWMDTVKPKRLIFSAGGKFDHPTQEAYDYYKDEGTLLAAAPHA
                     VTLSNATITVSTATYSTFDDGDIVVNVPGDTGSVDTYCVGPWGMLDALSLVSWCSPDL
                     "
     gene            complement(3076..3681)
                     /locus_tag="VFX11_08230"
     CDS             complement(3076..3681)
                     /locus_tag="VFX11_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938645.1"
                     /translation="MTISLADATAAVNAIKKIKGLQLVEKNKHLFEKHVSKAEALAAG
                     GNSVSWFEKKVAMKFSGDGDADKLSPSRLKKLAKVMNVGVRQVKPNLKKFDANTNDAY
                     SMEVTDDIGIYGASDLLEKALKQNSFDLYSCVENADSRFKYVTTIPAGYVGRRITASG
                     KQRLVDGLIVVIVNASSPVQVVTIYPVPQAWPTNTTDALLT"
BASE COUNT          887 a          953 c         1046 g          873 t
ORIGIN      
        1 aatttcagca atggcgagtg tggcaacaca ttcggcgccg gcatgctcat ggcatcggct
       61 gccgccgtct ccgatctgcc gtatccactg tacctgaccg atgcctacgt ccagacctcc
      121 atgttcgatt tcaacggaga cggagtgggc gacatcctgt acaccatgga ggtgccggtc
      181 attggggaag aaaacagtga gaatgattgg ctgatttctt atggcggcat gagtgaacct
      241 gaacttttac aaagtgaaga agagtttgta tcgcagcttg ttttcggtga cgttgatggc
      301 aatgggtcga gcgaaattgt ggtcgagcgg caggatgagg ctggttatca atggctggcc
      361 acgtccggtg agcgcaagtg ggtgaatgta ttgcccgcca gcgttcgctt tgtcagcgcc
      421 ggtgcagccg gttcccagat cgaagtgagc cacggcggta caaaaacagt aacgactctg
      481 ctggatctca atttcggcaa tttcagggga ggcccgagtt acgaagccct tctgactacg
      541 caaggcagcg atgcagtcac ccggtttttc atgtgcgaac tgccttcaac cgcgtgtgaa
      601 caggttgtga taacaggatt cgtcgcccaa cccgacagtc tttatatcgg gcagtttgac
      661 ggagatgctt accacgagat ttttgtgtcg cacgatgatg actggtatct ggtgcaaccg
      721 cagcagtccg gtggctgggt gactacgctg tatcgtgcca ataccatgaa tgatctcaag
      781 gatgcggatt ccgttggcgg ccagggctat gacgtcaacc agttcacgtc aaatatttcg
      841 aaatccctcc gcttcggtga ctttgatggc gatggcctca ccgatgtctt tacgtctcgc
      901 accaagaacg atctgggaca gtggaaatgg actgtgtctt atcggggagg agactgggag
      961 tatctcaacg ctgcgtggga acacccctcc atgttgagag tttccgactt cgacggggat
     1021 gcggtggctg acgttttcac cgcctcggaa accacccgcg atggtctggt ggactggagc
     1081 gtctgctacc ggggcaagag tacgtgggtt ccgctcaatg cgaataaaaa tctgcccttg
     1141 tcggtgttga gtctgggtga aacgaagttt gattgagcat tcgcaatggc actgaacaca
     1201 cttccacata tgacgggaaa tgcaatgaga ctggaacgcg taaccatttt caacgtccct
     1261 gcggcgattc tggccttgct ggctacctta ttcgcgtccg tggcacccga agcgcaggcc
     1321 tatacctatg acgaagcggc ggcaaaaaaa attctgtacg gattgaccgg tacgggtggc
     1381 cgcgcgtttg ataccacgaa ggcggtaaat tcctatgcgg tatttccaga gacaatgaag
     1441 gaggccgaca ttctggcgat cgtcaagaac gcgctggaag acgatgaaga ctgggaggag
     1501 tcggagatgg accggcgccg gcgcggcaaa acggtggagg tggataacct gagcgtgtac
     1561 attgaagtac agcacaaacc ggagaaaata ctggatttct atcccgtcgg tggatactgg
     1621 aaatcaaccg ataccgtctg ggcgaactat gccaaatcgc tgccgtccat gaagattggc
     1681 gttgcgcgga tttatcactg cagcgatttt tgtccaagca tgcttgaagg atggcacacc
     1741 gacaagggca ccctgatcaa cacggacaat gtcagcaaaa aagcgcgtaa atcgtctgat
     1801 cttaccttcc atatttacga cgtgggtgca ggcaactgca tcattgcgga atgtccggcg
     1861 gcaggcggag aaaataacgc cattattctg gactgcggtg tatccaaggc tcagctttca
     1921 tcaattgtga aacccagcgc gacattcggt ggcaaggctc cactgatgct ggaggacatg
     1981 tacgatattc tcgccaagaa agacaagata gatctcatcc tgacccatcc tgatatcgat
     2041 cactacaact tgattcccta tcttttctat tccaagaaaa agacagagac agaaaacgcc
     2101 attgcgccaa aactcaggag catctatgtg ggcgggctct ggacggacta caaagactct
     2161 gcgttgagcg ttgaagcggg tgcaccagcg cccaaaaggc gcaagaccgg tggggcaaag
     2221 gtacacgagg tgctaggcaa gatttccgac ggcacgatcc tggcttccaa accggcggtc
     2281 catgccatga tcaatgccaa cgacgtctac aagaattctt cttttaccgc acgatcgttc
     2341 aagggaaacg aaaatttggc ctgtggcgat gcggcaaaaa tactggcata caatgcctat
     2401 ccgaaccgga taggcaagga aaaaggagag gaatccaatg gccaaagtat cattgtcgcc
     2461 tttcagcacg gcggaaagcg catagccttg actggcgatg cgacggatgt ggggttgcgg
     2521 aacgccatca ccaatgatgc aaccaccggt tatttgaatg gggttgatgt actggtcgta
     2581 ccgcaccatg gttcacgtct accgacacat ttcccggccg aatggatgga caccgtaaaa
     2641 ccgaagcggt tgattttcag cgccggcgga aaattcgatc atccaaccca ggaagcctat
     2701 gactactaca aagacgaggg gacattgctg gcggcggcgc cccacgcggt taccttgtcc
     2761 aatgccacaa tcactgtcag tacggcaacc tatagcacct tcgatgatgg cgacattgtt
     2821 gtgaatgtac ctggcgatac gggtagcgtt gatacctatt gcgtgggtcc atggggaatg
     2881 ctcgatgccc tgtcgctggt cagctggtgt tcgccggacc tgtaacaatc tccgccggcc
     2941 ttgttgtacc agatttggta cgcgatcgtt ggcgatggcg gtcaggagag cgcttcgcgg
     3001 ctgatgggtg cttctgccat ggcagcgcaa atatcagtgc catggcaaat cgcttccctc
     3061 tgcgcgcgcc ccacatcaag tcagcagcgc gtccgtggta ttggtcggcc aggcctgggg
     3121 aaccggatat attgtcacca cctgcaccgg gctcgacgca ttgacaatga caacaatcaa
     3181 tccatccacc agcctttgtt tgccgctggc agtaatcctg cgcccgacgt aacctgccgg
     3241 aatagtggtg acatatttaa atcgggaatc tgcattttcg acgcagctat acaggtcaaa
     3301 actgttctgc ttcaacgctt tttccagcag gtcgctggcg ccgtaaatac cgatgtcatc
     3361 cgttacttcc attgaatagg catcgttggt attggcatcg aatttcttga gattcggttt
     3421 gacctgacgc acgcctacgt tcatcacctt cgccagtttc tttaagcggg atgggctcag
     3481 cttatcggca tccccatcgc ctgaaaattt catggcgacc ttcttttcga accagctgac
     3541 cgagtttcca cccgctgcaa gggcttccgc tttggaaacg tgcttttcga agagatgctt
     3601 gtttttttcc acaagctgca ggcctttaat tttcttgatg gcgtttacgg cggcggtggc
     3661 gtcggccagc gagatggtca tggaattctt tcctgtctgg agggggcggc ggcgttttgt
     3721 gaaaggcttt ctgactccaa tataacaaga gatccagac
//
LOCUS       DASUEG010000439         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1524653, whole genome shotgun sequence.
ACCESSION   DASUEG010000439 DASUEG010000000
VERSION     DASUEG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524653"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VFX11_08235"
     CDS             complement(<1..114)
                     /locus_tag="VFX11_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4938646.1"
                     /translation="MNVLVFDIETVPDLEGGRRLFGLEGLKDGDTADAMFHL"
     gene            complement(125..1018)
                     /gene="cysM"
                     /locus_tag="VFX11_08240"
     CDS             complement(125..1018)
                     /gene="cysM"
                     /locus_tag="VFX11_08240"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX4938647.1"
                     /translation="MHYPTIEDFVGNTPLVRLQRLPGDTTNTILVKLEGNNPAGSVKD
                     RPAMSMIQHAEARGLIKPGDTLIEATSGNTGIALAMAAAIKGYRMVLIMPDNMSEERR
                     AAMSAYGAELVRVTQKEGMEGARDLAEQMQRDGQGIVLDQFANQDNPLAHYETTGPEI
                     WRDTEGTITHFVSSMGTTGTIMGTSRFLKEKNPEIQIIGLQPQEGSAIPGIRRWPQAY
                     LPKIYDASRVDRVLDITQKDAEETMRALAAKEGIFCGVSSGGAVAGALQISREVENAV
                     IVAIICDRGDRYLSTGVFSPH"
     gene            1161..>2703
                     /locus_tag="VFX11_08245"
     CDS             1161..>2703
                     /locus_tag="VFX11_08245"
                     /inference="COORDINATES: protein motif:HMM:NF021499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4938648.1"
                     /translation="MKTDTKKIASLLPLKNVGLRTRALLISSLPVILIGFALAGTLML
                     QRFDELDKSLENQGKMIAAAFSHGIFESMTQRQGHTLPFYAQRILEEDNVRSVAIYDT
                     TGKLITQAGPRMHPLPEHEPLLPADSTKNGVSRLTDTTLRVIHPIYPASSYHPLASDR
                     PEAGWLEVEVDRSNLVLAKYRNLLSMLLVISLAVVLNTAIAVHFSASIGRVVSRIKQA
                     LAAMTSGNLDVRVEEDAYGELKELQAALNHLGQSILEGQQELQQNVDQATEDLRETLE
                     TIEIQNIELDLARKEALEASRIKSEFLANMSHEIRTPLNGIIGFTNLLLKSDVSLTQR
                     DYLDTIQKSSESLLAIINDILDFSKIEAGKLVLDQVPLNFQEVVEDVLTMLAPMAHEK
                     HLEQVSLFYSDVPTGIVGDPLRLKQILTNLVNNAIKFTDHGEIVVRVMLDDLKDNSAT
                     IKVSVSDTGIGLTKEQQRALFSAFRQADTTTARRFGGTGLGLVISKHLVEQMRGEVGL
                     ESEPGQ"
BASE COUNT          581 a          890 c          739 g          493 t
ORIGIN      
        1 caaatgaaac atcgcatcgg ccgtgtcgcc gtccttcaaa ccttccagcc cgaacagacg
       61 acgaccgccc tccagatcgg gcacggtttc aatatcaaac accagtacgt tcatggttac
      121 ccaatcaatg tggcgaaaac acgccggtgg aaagataacg atcaccgcga tcacaaatga
      181 tagccacgat cacagcgttt tcgacctcac gggaaatctg cagcgccccc gccaccgcgc
      241 caccggacga cacgccgcag aaaatgcctt ccttcgccgc cagcgcacgc atggtttcct
      301 cggcatcctt ctgcgtaata tccagcaccc gatccacccg tgatgcatca taaattttcg
      361 gcagataggc ctgcggccag cgccggatac cggggatcgc cgatccttcc tgcggctgca
      421 agccaatgat ctggatctcc ggattttttt ccttcagaaa acgcgacgtg cccatgatgg
      481 tgccggtcgt ccccatcgag ctgacgaaat gcgtgatcgt gccttcagtg tcacgccaga
      541 tctccgggcc cgtggtttcg tagtgggcca gcggattgtc ctgattggcg aactggtcca
      601 gcacaatgcc ctgaccatcg cgctgcatct gctcggccag atcccgggcg ccttccatgc
      661 cttccttctg ggtcacccgt accagctcgg caccgtacgc cgacatcgcc gcgcgccgct
      721 cctcgctcat gttgtcgggc atgatcagca ccatgcggta acccttgatc gccgccgcca
      781 tggccagcgc gatgccggtg ttgccgctcg tcgcttcaat cagggtgtca ccgggcttga
      841 tcaggccacg ggcctcggcg tgctggatca tgctcatcgc aggacgatcc ttgaccgaac
      901 cggcgggatt attgccctcc agtttcacca gaatcgtatt ggtggtgtcc cccggcagcc
      961 gctgcagccg taccagcggc gtgtttccta caaagtcttc aatcgtggga taatgcatgg
     1021 ctacgtcccg aacccgtgaa atgcggctat tgtaccctgc acccgggagc gagcctatca
     1081 ccggcaggcg aaatagcttt ggcaagttgg catatactta gaacgaaaaa ccggtgccct
     1141 gtcctggctg gaaatcccgc gtgaaaacag acacaaagaa gattgcctcc ctgctgcccc
     1201 tgaagaacgt gggcctgcgg acccgtgccc tgctgatcag ctcactgccg gtcatcctga
     1261 tcggctttgc gctggcgggc accctgatgc tgcagcgctt cgacgaactg gacaagtcgc
     1321 tggaaaacca gggcaagatg atcgcggccg ccttttccca cggcatcttc gaaagcatga
     1381 cccagcgcca gggccacacc ctgccgtttt acgcccagcg catcctggaa gaggacaacg
     1441 tccgctcggt ggccatctac gacaccacgg gcaagctgat cacccaggcc ggcccccgca
     1501 tgcatccgtt accggagcac gaacccctgc tgccggcgga ttccaccaag aacggcgtca
     1561 gccgcctgac cgacaccacc ctgcgggtga ttcacccgat ctacccggcc tcttcctatc
     1621 atccgctggc cagcgaccgg cccgaggcgg gctggctgga agtggaagtc gaccgctcca
     1681 atctggtgct ggccaagtac cgcaacctgc tgtcgatgct gctggtgatc tcactggcag
     1741 tggtgctgaa caccgccatc gccgtgcatt tttctgccag catcggccgc gtggtgtcgc
     1801 gcatcaaaca ggccctggcc gccatgacca gcggcaacct ggatgtgcgg gtggaagaag
     1861 atgcctatgg tgaactgaag gaactgcagg cggcgctgaa ccatctgggc cagtcgatcc
     1921 tggaaggcca gcaggaactg cagcagaacg tggaccaggc caccgaagac ctgcgcgaaa
     1981 cccttgaaac catcgagatc cagaacatcg aactggatct ggcgcgcaag gaagcgctgg
     2041 aagccagccg catcaaatcc gaattcctgg ccaacatgtc ccacgaaatc cgcacgccgc
     2101 tcaacggcat catcggcttc accaacctgc tgctgaaaag cgacgtcagc ctgacccagc
     2161 gcgactacct cgacaccatc cagaaatcct ctgaaagcct gctggccatc atcaacgaca
     2221 ttctcgactt ctcgaagatc gaagcgggca agctggtgct ggaccaggtg ccgctgaatt
     2281 tccaggaagt ggtggaagac gtgctcacca tgctggcgcc gatggcgcac gaaaaacatc
     2341 tggagcaggt cagcctgttc tattccgacg tacccaccgg catcgtcggc gacccgttgc
     2401 ggctgaaaca gatcctcacc aacctggtga acaacgccat caagttcacc gaccacggcg
     2461 aaatcgtggt acgggtgatg ctggacgatc tgaaagacaa cagtgccacc atcaaggtca
     2521 gcgtatccga caccggcatc ggccttacca aggaacagca gcgcgccctg ttcagcgcct
     2581 tccgccaggc cgacaccacc actgcgcgac gcttcggcgg taccggtctg ggactggtga
     2641 tttccaaaca cctggttgaa cagatgcggg gcgaagtggg cctggaaagc gaacccggtc
     2701 agg
//
LOCUS       DASUEG010000440         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1244548, whole genome shotgun sequence.
ACCESSION   DASUEG010000440 DASUEG010000000
VERSION     DASUEG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244548"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFX11_08250"
     CDS             complement(<1..224)
                     /locus_tag="VFX11_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4938649.1"
                     /translation="MKNFNNKVAAITGAASGIGRSLALALAREKCDLALSDVNETGLQ
                     ETAEKARALGVKVTTAKVNVADRAAMHAWA"
     gene            complement(221..1771)
                     /locus_tag="VFX11_08255"
     CDS             complement(221..1771)
                     /locus_tag="VFX11_08255"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4938650.1"
                     /translation="MTAQSAASRGYAEHHKIVIVGSGFSGLGMGIKLKEAGENDFVLL
                     EKESGVGGTWRVNHYPGCACDVPSHLYSFSFEANPGWSRMFAPQQEIKAYLEHCARKY
                     NILPHVKLNTTVESLRWDEHRGLWRIHCAGGAEYTANVVVSGMGGLSIPAYPNVKGLD
                     QFQGKMFHSQQWDHSYDLTGKRVAVIGTGASAIQFVPYVQKKAAKLDLYQRTAPWIMS
                     KPDRAMTWVEKNLFKRFPAAQRAFRDGIYWALESRVLAFVINPRLMAIAKRDAMKHIR
                     NQISDPVLRKKVTPDYTIGCKRILMSDEYYPALAAPNADVITTGIQEVRANSIVTVDG
                     VEHPADAIIFGTGFKAADPIPPGVIFGRDGKDLGELWKAGPEAYKGTTVAGFPNLFFI
                     MGPNTGLGHNSMVYMIESQIAYVLEALKLINKKRLRSVEVNARAQASFNAEIQGKVAG
                     TVWNAGGCQSWYIHPQSGKNVTLWPGFTWQFRKITRRFDAGAYHLASDGAAVVANKNE
                     SLINEVSA"
     gene            1908..2636
                     /locus_tag="VFX11_08260"
     CDS             1908..2636
                     /locus_tag="VFX11_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4938651.1"
                     /translation="MTARKPSLTKTVAAQGKRLLMDAALKLTAQSRSINALGLRELAR
                     EAGLNPNTFYRHFKDIDELGLTIIEEMSNKLRQPLRDLRRQAAESVVPPELAGIGWEE
                     NPLLNLQKATLATKETVKLFFDYVAQNPNAFILGVRELHGASPLLRQALRKVMADFAQ
                     DMADDIKQLKLLPMLDDATLLEVASTISREMFQLSMDYIEQPEQRNAIFAQAEALVVM
                     LFTGATVLKGHGRLLMQALDPLPA"
BASE COUNT          574 a          842 c          772 g          568 t
ORIGIN      
        1 tcggcccagg cgtgcatcgc tgcgcggtcg gccacgttca ctttggcggt ggtgactttc
       61 acgcccaggg cacgggcctt ttccgcagtt tcctgcaggc cggtttcatt cacatcgctc
      121 agcgccagat cgcatttttc acgcgccagt gccagcgcca gggaacgacc gatacccgat
      181 gcagccccgg taatggctgc cactttgtta ttgaagttct tcacgcagac acctcattga
      241 tcaacgactc atttttattg gcgacgacgg cagcaccgtc gcttgccaga tggtaggctc
      301 cggcatcgaa gcggcgcgtg atcttgcgga attgccaggt gaaacccggc cacaatgtca
      361 cgttcttgcc gctctgcgga tgaatgtacc agctctggca accgccggcg ttccacaccg
      421 tgccggctac cttgccttga atttccgcat tgaaagatgc ttgcgccctg gcattcactt
      481 ccacgctacg cagacgcttt ttattgataa gtttgagcgc ttccagcaca tacgcaatct
      541 gcgattcgat catgtatacc atggaattat ggcccagtcc tgtgttcgga cccataatga
      601 aaaacagatt cgggaatcca gccacagtcg tgcctttgta ggcttcgggg cccgctttcc
      661 acagctcgcc cagatccttg ccgtcgcgtc cgaaaatcac gccgggcgga atcggatcgg
      721 ccgccttgaa accggtgccg aagatgatcg cgtcagcggg atgttcgacg ccatccaccg
      781 tgacgatgct gttggcgcgc acttcctgga tgcctgtggt aatcacatcc gcgttgggtg
      841 ccgccaatgc cggataatat tcatcggaca tcaaaatgcg cttgcagccg atggtgtaat
      901 ccggcgtcac tttcttgcgc aacaccggat cgctgatctg gttgcggata tgcttcatcg
      961 catcgcgttt tgcgattgcc atcaaacgcg gattgatgac gaaggccagc acgcgacttt
     1021 ccagcgccca gtaaatgccg tcacggaatg cacgctgcgc ggcgggaaaa cgcttgaaca
     1081 gatttttttc cacccaggtc atggcgcgat ccggcttcga catgatccag ggcgcggtgc
     1141 gctggtacag atccagtttc gccgccttct tctgcacgta cggtacgaac tggatcgccg
     1201 acgcgccggt gccgatgacg gccacgcgct tgccggtcag atcgtaggaa tgatcccact
     1261 gctgggaatg aaacatcttg ccctggaact gatccagccc tttcacattc gggtacgccg
     1321 gaatcgacag gccacccatg ccggacacca cgacattcgc ggtatattcc gcaccgcctg
     1381 cgcagtgaat gcgccacaga ccgcgatgct cgtcccagcg cagggattcc acagtggtgt
     1441 tcagcttcac atggggcagg atgttgtatt tgcgcgcgca atgttccaga taggccttga
     1501 tctcctgctg cggcgcaaac atgcggctcc agcccggatt ggcctcgaac gaaaacgaat
     1561 acaggtggga cggcacatcg caggcacaac cgggataatg attcacgcgc caggtaccgc
     1621 cgacgcccga ttccttctcc agcaggacga aatcattttc gccggcttct ttcagtttga
     1681 tccccatgcc caggcccgaa aaacccgagc cgacgatgac gattttgtgg tgttcggcat
     1741 accctctgct tgcggcggac tgcgctgtca tgactctggt cccggtgtta ttgatattgt
     1801 gagaaggtag acaactgtct acacccgatc aatcgtatac agttgtatac ttaccggtca
     1861 agcgcacgta caataaataa caacttttag ccagaatcga tcaaatcatg accgctcgta
     1921 aacccagcct caccaagacc gttgccgccc agggcaaacg cctgctgatg gacgccgcgc
     1981 tgaaactcac ggcccagtcc cgcagcatca atgctctggg attgcgggaa ctggcgcggg
     2041 aggccggcct caaccccaac actttctatc gtcatttcaa ggatatagat gaattggggc
     2101 tgaccatcat cgaggagatg tcgaacaagc tgcggcagcc cctgcgggac ctgcggcggc
     2161 aggcagcaga gtcggtggtg ccgccggagc tggcgggcat cggctgggag gagaacccgc
     2221 tgctgaacct gcagaaggcc accctggcca ccaaggagac ggtgaaactg tttttcgact
     2281 atgtggcgca gaaccctaac gcgttcatcc tgggcgtgcg cgaactgcac ggcgcgtcgc
     2341 cgctgctgcg gcaggcactg cgcaaggtga tggcggattt tgcgcaggac atggcggacg
     2401 acatcaagca gctgaaactg ctgccgatgc tggacgatgc aacgctgctg gaagtggcat
     2461 ccaccatcag ccgcgaaatg ttccagctgt cgatggacta catcgagcag ccagaacagc
     2521 gcaacgcgat tttcgcgcag gccgaagccc tggtggtgat gctgttcacc ggtgcgaccg
     2581 tattgaaggg ccacgggcgt ttgctgatgc aggcgctgga cccgctaccg gcctgaaaaa
     2641 acgacggcgg agaatctcag ttgcccgcca gcacagactg cgccagtgct tccgccaccc
     2701 ggatgccgtc cacgcccgcc gacagaatgc caccggcata accggcgcct tccccc
//
LOCUS       DASUEG010000441         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1238677, whole genome shotgun sequence.
ACCESSION   DASUEG010000441 DASUEG010000000
VERSION     DASUEG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238677"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..843)
                     /locus_tag="VFX11_08265"
     CDS             complement(133..843)
                     /locus_tag="VFX11_08265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4938652.1"
                     /translation="METKSIRFRQVIDLSHDITTSIPLWPGDPPVEFETVATVETNGY
                     FLRRFSIGEHSATHMNAPNTFYGDGKGIDGYEPGQLVRPAVVIDVRQQTAQNPDYVIT
                     VQDVKRWEQQHGRIRRGSLVLFYTGWQERWHSPQQFFNADANGGLHFPGIGGDTSKFL
                     LEKRHIAGVGIDTHGVDAGQDTAYATNLQVLAANGIVLECLTRLDQLPPKGATLVIGV
                     LRLEGGSGSPVSVMAFLP"
     gene            complement(1253..1819)
                     /gene="dcd"
                     /locus_tag="VFX11_08270"
     CDS             complement(1253..1819)
                     /gene="dcd"
                     /locus_tag="VFX11_08270"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX4938653.1"
                     /translation="MSIKSDRWIRRMAEQHGMIEPFEPGQVRTANGQRIISYGTSSYG
                     YDVRCSREFKVFTNIHSATVDPKDFSDKSFVDIESDVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYANEGVAQMLFL
                     ESDEECETSYRDRGGKYQGQTGVTLPKT"
     gene            complement(1903..2481)
                     /locus_tag="VFX11_08275"
     CDS             complement(1903..2481)
                     /locus_tag="VFX11_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX4938654.1"
                     /translation="MGKRILILNGNPKPDSLCAGLAAAYGDAAAASHEVRPVALAQLG
                     FESDLHAGYDRDQPLEPDLHTFQQNLLWAQHLVLVAPVWWGGVPARLKGLIDRAFLPG
                     FAFRYEKGQVWPTQLLKGKTATLLLTLDTPPWYYRLLQGAPAVRQLDTATLAFSGFKP
                     VRHHLFGPVIHADESRRQRWLDKAQVLGARAG"
     gene            complement(2539..2925)
                     /locus_tag="VFX11_08280"
     CDS             complement(2539..2925)
                     /locus_tag="VFX11_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010384743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4938655.1"
                     /translation="MYIGTAARKTGLSVKAIRFYEAVGLIPSPPRAGRYRHYDAHSIE
                     LLLLIKEAKALGVTIAELKRLVQGADGPLDWGKVATFLQQQKVRVRAEIQTLEAQLER
                     IDTCLGLIAECPDVSPSAPSGSGIAC"
     gene            complement(2950..4041)
                     /gene="apbC"
                     /locus_tag="VFX11_08285"
     CDS             complement(2950..4041)
                     /gene="apbC"
                     /locus_tag="VFX11_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX4938656.1"
                     /translation="MNSQLHEQVSRILATQPDPVSRQNLVDVADLTEVSLSGDTLNLG
                     LQFGYPINGVREQLQAELAPLFSKLGAAKVNLKLDWKVKPALTKVLEGAGKVRNILAV
                     ASGKGGVGKSTTAVNLALALAADGARVGILDADIYGPSQPLMLGVPEGTKPQVHQQTF
                     FLPIDALGLQSMSMGYLVTEQTPMVWRGPMASGALQQLLKQTIWSDLDYLVVDMPPGT
                     GDIQLTLSQQVPVSGAVVVTTPQDIALLDAKKGVEMFRKVNVPVLGIVENMAIHICSN
                     CGHQEHIFGAGGGEKIAKQYGVSVLGSLPLSLSIREQADGGKPTVAADPNGEIAAIYR
                     RIARLAAGQLAKMSRQSTGAFPKISIHND"
     gene            complement(4227..>4834)
                     /locus_tag="VFX11_08290"
     CDS             complement(4227..>4834)
                     /locus_tag="VFX11_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010634324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX4938657.1"
                     /translation="LPGLEAGSEILGHKGWAAAVVVFGMALGVLLMLGLDAFTPHEHD
                     KTGPCGPGHDECGRIWLFVFAIALHNLPEGMAIGVAFSQADMAVGLPLTTAIALQDIP
                     EGLAVALAIRGAGYSPLLAVLVAGASGLLEPLGSLLGVGLSSGMAVAYPVGLGLAAGA
                     MLFVVSHEVIPETHRNGHQTSATVGLMLGFALMMVLDTTLG"
BASE COUNT          970 a         1573 c         1423 g          868 t
ORIGIN      
        1 gactacctca ccaaacccct gcgcctggag gaactggtca acgcattgcg gcgggcggcc
       61 cgtcacgtca cccgccaacg cgaaaccgcg ctgtaaccct gccggcatca cgcgcgccgg
      121 caggggccac gatcagggca gaaacgccat caccgacacc ggcgaaccgg aaccaccttc
      181 cagccgcaac acgccgatca ccagcgtggc gcccttcggc ggcaactgat ccagccgggt
      241 cagacactcc agcacaatgc cattggccgc cagcacctgc aggttggtgg catatgccgt
      301 gtcctgccct gcgtcaacgc cgtgcgtgtc aatgccgacg cccgcgatat ggcgcttctc
      361 cagcaggaat ttcgaggtgt ccccgccaat gccgggaaaa tgcagtccac cgttggcgtc
      421 cgcattgaaa aactgctgtg gcgagtgcca gcgttcctgc cagccggtat aaaacagtac
      481 cagcgacccg cgccggatgc ggccatgctg ctgctcccag cgcttcacat cctgcaccgt
      541 gatcacgtaa tctggattct gcgccgtctg ctgccgcaca tcgatcacca ccgccggacg
      601 caccagctga cccggctcgt agccatcgat gcccttgccg tccccgtaaa atgtattggg
      661 tgcattcatg tgagtggcgc tgtgctcgcc gatggaaaaa cgccgcagga aataaccgtt
      721 ggtttctacc gtcgccactg tttcgaattc cacgggcgga tcgccaggcc agagtggaat
      781 gctggtggta atgtcgtgac tcagatcgat cacctgcctg aaacggatcg attttgtttc
      841 catgtcccct gctactcctg ttgtcgccac actggacagc cagagcgcga atcccatggc
      901 catccatcgt tttggctttg caaaaaaata ctgagtgctg aatacgatca ttgcgtttcc
      961 tctccattgc gtcagtaaga aaacctcgaa gcaggctttc ctattagcag aatagaacag
     1021 aaacggatcg gtcaataatc aattcgggtg tcggcgcatg tttgcagtaa cactcgcaac
     1081 agcgatgacg caacgggagg cagactgtcg aacaactgtc ttgtcgtaca gacggaatgc
     1141 catgaccgtg caaaccgtag cgctgtcact aagccgccgc tgacaggccg ccgatgcatc
     1201 cctgatgtaa aaaatgtgac agcgaagacg ggctgtcagg ttttcggcgc aatcaggttt
     1261 tgggaagcgt cacgccagtc tggccctggt attttccgcc gcgatcgcgg tacgaggttt
     1321 cgcattcctc gtcggattcc aggaacagca tctgcgccac gccttcgttg gcgtagattt
     1381 tcgccggcag cgtggtggtg ttggaaaatt ccagcgtcac atggccttcc cactccggct
     1441 ccagcggcgt cacgttgacg atgatgccgc agcgggcgta ggtggacttg cccaggcaga
     1501 tggtcagcac gttgcgggga atgcggaagt attccacggt ccgcgccagg gcaaaggagt
     1561 tggggggaat gatgcagacg tcgctttcga tatcgacgaa gctcttgtcg gagaaatcct
     1621 tgggatcaac cgtggcggaa tggatgttgg taaacacctt gaattcccgc gagcagcgca
     1681 cgtcatagcc gtaactcgac gtgccatagg aaatgatgcg ctggccattg gcggtacgca
     1741 cctgacccgg ctcgaagggt tcgatcatgc cgtgttgttc tgccatgcgg cggatccagc
     1801 gatcagactt gatggacatc gaattcaaac tcccccggaa atggaagcgc gcatgataca
     1861 gggttttggc gccggccaga accctgttcc gggtgaaccc ggtcagcctg cccgggcgcc
     1921 cagcacctgg gccttgtcca gccagcgctg acgccgggat tcatcggcat ggatcaccgg
     1981 cccgaacagg tggtgacgca ccggtttgaa gccactgaag gccagcgtgg ccgtgtccag
     2041 ctgccgcacg gccggcgcgc cctgcagcag gcggtagtac cagggcggcg tatccagggt
     2101 cagcagcagg gtggcggttt tacccttcag caactgcgtc ggccacacct gtcctttctc
     2161 gtagcggaaa gcaaaacccg gcaggaaggc gcggtcgatc aggcccttca gccgggccgg
     2221 cacaccaccc caccagaccg gcgccaccag caccagatgc tgggcccata acagattctg
     2281 ctggaaggtg tgcaggtccg gctccagcgg ctgatcccgg tcgtaacctg catgcagatc
     2341 actctcgaaa cccagttgcg ccagcgccac aggccgtacc tcgtggctgg ccgcagcagc
     2401 atcgccatag gcggcggcca gaccggcaca caggctgtct ggcttgggat tgccgttcag
     2461 gatcaggatg cgtttgccca ttgcatgctc cggtagtcga tgacgccggc agtatgcact
     2521 ctgccctaaa ggggagagtc aacaggcaat gccggaaccg gacggcgcag acgggctgac
     2581 atcaggacac tccgcaatca gcccgaggca ggtgtcgatc cgctccagct gcgcttccag
     2641 cgtctgtatt tccgcacgca cccgcacttt ctgctgctga agaaacgtcg ctaccttgcc
     2701 ccagtccagc ggaccgtccg cgccctgaac cagccgtttc aactcggcga tagtcacccc
     2761 cagagccttg gcttccttga tcagcaacag cagctcaatt gaatgggcgt cgtaatggcg
     2821 atagcgcccc gcccggggag gcgacggaat caaacccacc gcttcgtaaa agcggatggc
     2881 tttcactgaa aggccagtct tgcgcgctgc tgtgccgatg tacatccggc gcctccttgc
     2941 gaaacaggtt cagtcgttgt ggatgctgat cttggggaag gcaccggtgg actggcgcga
     3001 catcttcgcc agctgccccg ccgccagccg cgcgatgcga cggtagatgg ccgcgatctc
     3061 gccattggga tcggccgcca cggtgggctt gccgccgtcc gcctgctcgc ggatcgacag
     3121 gctcaggggc aggctgccca gcaccgacac gccgtactgt ttcgcaatct tctcgccgcc
     3181 gccggcaccg aaaatatgct cctggtgacc gcaattgctg cagatatgga tcgccatgtt
     3241 ctccacaatt cccagcaccg gcacattcac cttgcggaac atctccacgc cctttttcgc
     3301 gtccagcagc gcaatgtcct gcggcgtggt caccacgacg gcgcccgaca ccggcacctg
     3361 ctgcgacagc gtcagctgga tgtcgccggt gccggggggc atatccacca ccagataatc
     3421 caggtcagac cagatcgttt gtttcagcaa ctgctgcagc gcgccgctgg ccatggggcc
     3481 gcgccacacc atcggggtct gctcggtaac cagatagccc atcgacatgg actgcaggcc
     3541 cagcgcgtca atcggcagga aaaacgtttg ctggtgcacc tgcggcttgg tgccctcggg
     3601 cacccccagc atcagcggct ggctggggcc ataaatgtcg gcatccagaa tacccacccg
     3661 ggcaccatcg gcagccaggg ccagagccag gttcaccgcg gtagtggact tgcccacacc
     3721 gcccttgccc gacgccaccg ccaggatatt gcgtaccttg cccgcgcctt ccagcacctt
     3781 ggtcagggcc ggcttcacct tccagtccag cttcagattg accttcgccg cacccagctt
     3841 gctgaacagg ggcgccagtt ccgcctgcag ctgttccctc acaccgttga tggggtaacc
     3901 gaactgcaga cccaggttca gggtatcgcc gctcagggaa acttctgtca ggtctgcgac
     3961 atccaccagg ttctgacgcg acaccggatc gggctgggtg gcaaggatgc ggctgacctg
     4021 ttcatgcaac tgggaattca tggggactgc tccaatggtt tcgttctggc cggtcggatc
     4081 acattcggcg ttttgcgaaa tgcgggccaa cgggcaaccg ggaaataaaa cgggtgacag
     4141 ggaagcaaag aggcgacccc gtgtcgaggc cgcccatgct aaaccaaaat accccactgg
     4201 aacagtcggg tacccgttgg aacgcctcag cccaacgtcg tgtcgagcac catcatcagg
     4261 gcgaaaccca gcatcaggcc cacggtggcc gaggtctggt ggccgttgcg gtgggtttcc
     4321 ggaatcactt catgggacac cacaaacagc atcgccccgg ccgccaggcc cagtccaacc
     4381 ggataggcca cggccatacc gctggacaga cccactccca gcagcgaccc caatggttcc
     4441 agcaacccgc tggcgcccgc caccagcact gccagcaacg gcgaatagcc ggcgccgcga
     4501 atagccagcg ccaccgccag accttcggga atatcctgca acgcgatagc cgtggtcagc
     4561 ggcaagccca ctgccatgtc agcctgggaa aaggcgacgc cgatggccat gccctccggc
     4621 aggttgtgca gcgcgatggc aaacacgaac agccagatgc ggccgcactc gtcatggccc
     4681 gggccgcagg gaccggtctt gtcgtgctcg tgcggcgtga aggcgtcgag gccgagcatc
     4741 agcagcacgc cgagcgccat gccgaacacc accacggcgg cggcccagcc cttgtggccg
     4801 aggatttcgc tgccggcctc gaggccgggc agca
//
LOCUS       DASUEG010000442         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2121312, whole genome shotgun sequence.
ACCESSION   DASUEG010000442 DASUEG010000000
VERSION     DASUEG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121312"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFX11_08295"
     CDS             complement(<1..727)
                     /locus_tag="VFX11_08295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4938658.1"
                     /translation="MTTAAKTSNNLNGASVGIPPRHIDFNFSQSIPKYFYAGNATATT
                     FFAMLSGFFPPGERYFMDSVRHFRNRVTDKAQRAAISGFMGQEAIHGREHDRLNELLA
                     ERGFDMRTPERFVKIGLKVLEMLPPSTKLAATTFMEHFTALLAEQLLEDKDFQSLADP
                     EMIKIWQWHALEELEHKSVAYDVYELVGNRYSERVAATAASLVVLLPMLGVTWAWMLA
                     RDGKLGSIKDNAKGLNMLFGRNGF"
     gene            complement(763..1620)
                     /locus_tag="VFX11_08300"
     CDS             complement(763..1620)
                     /locus_tag="VFX11_08300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938659.1"
                     /translation="MKNFSGKVAAITGAGSGIGQATAIALAKSGCHLSLSDVNDKGLA
                     QTLDKLQPYRVKVTLHHVDVGDRAAVYQYAEDTVKEHGQVNLIINNAGVGLGETVEAM
                     SYENFEWLMNINFWGVVYGTKAFLPHLKQSGDGHVVNISSIFGIIGVPTQSAYNAAKF
                     AVRGFTESLREELDLEGGCVSATCVHPGGVKTNIARNSRMGEMGSMSMGGQDEIAEMF
                     DRIAMSTPEQAAKAILGGVQKNQRRVLIGADAVLMDTTQRLMPTGYQRALETLFRMNK
                     GRGAKATTV"
     gene            complement(1648..>2378)
                     /locus_tag="VFX11_08305"
     CDS             complement(1648..>2378)
                     /locus_tag="VFX11_08305"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4938660.1"
                     /translation="PKAIRRLLLAATRRQLGPNVDMRHFQPTYNPWEERMCAVPKGDL
                     FKVIREGKASVVTDHIDTITETGIKLKSGDHLDADVLITATGLDLQLFGGATLRVDGR
                     EVSVADTLLYKGLMLEGVPNMALVFGYTNASWTLKADIACEFVCRLLNHMRDIGATRV
                     TPTDEEGCGTDMNFLNLRSGYVQRASDRLPRQGSRIPWQNLDDYLRDLPALRYGNLED
                     GYLHFEGENLREKKQGILGALLGA"
BASE COUNT          509 a          668 c          642 g          559 t
ORIGIN      
        1 caaacccgtt gcggccaaac agcatgttca ggcctttggc attgtctttg atgctgccga
       61 gcttgccatc gcgggccagc atccatgccc atgtgacacc gagcatcggc agcagcacga
      121 caagcgaggc cgcagtagcc gcgacccgtt cactgtaccg gttcccgact agttcataga
      181 cgtcgtaggc tacagacttg tgctcgagtt cctccagcgc atgccattgc catatcttga
      241 tcatttcagg gtcggccaga ctctgaaaat ccttatcctc aagcagctgc tctgccagca
      301 gggcggtaaa gtgctccatg aaggtggtgg ctgcgagctt ggtgctcggc gggagcattt
      361 ccagaacttt caggccaatt ttaacgaatc gctcgggcgt gcgcatgtcg aagccacgct
      421 cggcaagcag ttcgttcagg cgatcatgtt cgcgcccgtg gatggcctcc tgacccataa
      481 atccggaaat ggcggcacgc tgcgccttgt cggtaacccg atttcgaaag tggcgcaccg
      541 aatccatgaa gtaacgctca cccggcggga aaaaccctga cagcatggcg aagaacgtgg
      601 ttgccgtggc attcccggca taaaagtact tcgggatgct ttgtgagaaa ttaaaatcga
      661 tgtgacgcgg cgggatgccc accgaggcac cgttgaggtt gttgctggtt ttcgcggctg
      721 tagtcattgc gatactcctt agttcataat cgaaatgaga agttaaacag tggtcgcttt
      781 tgcgccgcga cctttgttca tccgaaacag tgtttcaaga gcacgctgat acccggtggg
      841 catcagccgc tgggtggtgt ccatcagtac cgcatcagcg ccgattaaca cgcgcctttg
      901 gttcttttgc actcccccga ggattgcttt tgcggcctgt tccggggtag acatggcgat
      961 gcggtcaaac atttcggcaa tctcgtcctg accacccatg ctcatactgc ccatttcacc
     1021 catgcggcta ttacgggcaa tgttggtctt gacgccgccg ggatggacac aggtggcgct
     1081 gacgcagccg ccttcgagat cgagttcttc gcgcaacgat tcagtaaagc ctctgacggc
     1141 gaacttcgct gcattgtagg cagactgggt tggtacaccg atgatgccga atatgctgga
     1201 gatgttgacc acatgaccgt cgccggactg cttcaggtgc ggaagaaaag ccttggtgcc
     1261 atataccaca ccccagaagt tgatattcat gagccattca aaattttcat agctcatggc
     1321 ctccaccgtt tccccgagac caacaccggc attgttgatg atcaggttca cctggccatg
     1381 ttccttgaca gtgtcctcag cgtactgata aaccgcagca cgatcaccga catcaacatg
     1441 atgcaacgtt accttcactc gatagggctg gagcttgtcc agggtttgcg ccaatccctt
     1501 gtcattgacg tctgaaaggg agagatggca accgcttttt gccagggcaa tggcggtggc
     1561 ctgaccgata cccgatccgg ccccggtgat ggcagcaact ttgccggaaa aatttttcat
     1621 gatatttcct cttgttaaaa ctgtcgttca ggcgccgagc agcgcaccga gtattccctg
     1681 ctttttttcc ctgaggtttt cgccttcgaa atgaagataa ccatcttcga gattgccata
     1741 gcgcaaggca gggaggtccc tcaggtaatc atccagattc tgccagggta tcctgcttcc
     1801 ctggcgtggc agtctgtcgc tggctcgctg aacatagccg gagcgcaggt tgaggaaatt
     1861 catatcggtg ccgcaaccct cctcatcagt gggggtgacc cgggtggcac caatgtcacg
     1921 catatggttg agtaagcggc agacaaattc gcaggcaata tcggctttca atgtccagga
     1981 ggcattggta tagccgaaca ccagcgccat gttaggcaca ccttccagca tcagtccctt
     2041 gtacaacagg gtgtcggcca cactgacttc acgaccgtcg acacgcagcg tagcaccgcc
     2101 aaacaactga agatccagac cggttgcggt aatcagaaca tccgcgtcca gatggtcgcc
     2161 ggacttgagc ttaatgcctg tttccgtaat cgtgtcgata tggtctgtga ccaccgatgc
     2221 cttgccttcg cggataacct tgaagaggtc tcctttcggg accgcgcaca tccgctcttc
     2281 ccaagggttg tacgttggct ggaagtggcg catgtcaaca ttcgggccga gttgcctgcg
     2341 cgttgcggcc agcaacagtc tgcgtattgc tttcggct
//
LOCUS       DASUEG010000443         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1630476, whole genome shotgun sequence.
ACCESSION   DASUEG010000443 DASUEG010000000
VERSION     DASUEG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630476"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFX11_08310"
     CDS             complement(<1..492)
                     /locus_tag="VFX11_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX4938661.1"
                     /translation="MTERTRIALLIDCDNVSHNAIEGVLEELAKNGMVNVRHAHGDWK
                     NPSLSGWVDRLHPHAIRPMQQFAYTKGKNATDAAMIIDAMDLLYSKNVDAFALMTSDS
                     DFTPLVMRILESGLPVYGFGEQKTPKPFVDACSTFIYTENLVPEKESKAESTKTSVKK
                     SKAE"
     gene            complement(509..868)
                     /locus_tag="VFX11_08315"
     CDS             complement(509..868)
                     /locus_tag="VFX11_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012069081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX4938662.1"
                     /translation="MTIVAAIVVVLVALLHVYFMVLETALWDKPAGLKAFGQTKEQAG
                     ATKVLAANQGLYNGFLAAGLLWGVIQGAAGNDFKVFFLLCVIAAGIVGALTASRKILY
                     VQALPGLVGLILVMISA"
     gene            complement(940..1317)
                     /locus_tag="VFX11_08320"
     CDS             complement(940..1317)
                     /locus_tag="VFX11_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938663.1"
                     /translation="MELDKSTKRIAKKAKKGFQGYPVIAIAYYGPHDKLATKVAIRFV
                     EEENSEPQTECFRTDGDIREDATIQSAIIKLIDRSAAKTVSVAGGIIGCPHEEGVDYP
                     AGQECPQCEFWRGRQGAADRSVK"
     gene            1727..2701
                     /locus_tag="VFX11_08325"
     CDS             1727..2701
                     /locus_tag="VFX11_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4938664.1"
                     /translation="MSNRDAILALKESLCEQIIGQQRLVDRLLIALLADGHLLVEGAP
                     GLAKTKAIKSLAQGVEGDFHRIQFTPDLLPADVTGTEIYRPQDSSFVFQPGPIFHNLV
                     LADEINRAPAKVQSALLEAMGERQISVGKYTHKLPRLFLVMATQNPIEQEGTYPLPEA
                     QLDRFLMHVTIGFPDAASEKKILQLARMEALEKQAPDDGVPPPIHITQQQIFDARSEI
                     LQLHMIPAIEEYIVQFTMATRNPLAYGDDLSGWIEYGVSPRGTIALDSCARAHAWLNN
                     RDFVTPDDVQAVAHDCLRHRLGLTFEAEAKGVTADHIIDELIDRVSAP"
     gene            2712..>2921
                     /locus_tag="VFX11_08330"
     CDS             2712..>2921
                     /locus_tag="VFX11_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4938665.1"
                     /translation="MASVNKLLHERADIAQYRGVYVSMDELVSLQGAELRLDLRGKRK
                     SLAGLAGQHRSSFRGRGMDFDEVRIY"
BASE COUNT          631 a          911 c          781 g          598 t
ORIGIN      
        1 ctccgccttg cttttcttca ccgacgtttt cgtgctttcc gccttggatt ccttttccgg
       61 aaccagattt tcggtataga taaaggtcga gcaggcatcc acgaacggtt taggcgtttt
      121 ctgttcgcca aagccataga ccggaagacc gctttccaga attctcatca caaggggagt
      181 aaagtcgctg tcgctggtca tcagtgcgaa tgcatcgaca ttcttgctgt aaagcagatc
      241 catggcatcg atgatcatgg cggcatcggt cgcattcttg cccttggtgt aggcaaactg
      301 ctgcatcggc ctgatggcat gcggatgcag acgatcaacc cagcccgaca gcgatggatt
      361 tttccagtct ccgtgcgcat gccgcacatt caccatgccg ttcttggcga gctcctccaa
      421 caccccttct atcgcattgt ggctgacgtt gtcgcagtcg atgagcagtg caatccgggt
      481 tctttccgtc acgtgtccac cctcgcagtc aagccgaaat catcaccagg atcagtccca
      541 ccagcccggg caacgcctgg acatacagaa tcttccggct tgcggttaat gcgccaacaa
      601 tgccggcggc aatcacacac aacaggaaaa acaccttgaa atcgttgccg gcggcgccct
      661 gaatcacgcc ccacagcaat ccagcggcga gaaacccgtt atatagcccc tggttcgcgg
      721 ccagcacttt tgtcgcgcct gcctgttctt tggtttggcc gaaggccttc agtccggccg
      781 gcttatccca aagggccgtt tcgagaacca tgaagtaaac atgcagcaag gccaccagca
      841 ccaccactat tgcagcaaca attgtcataa gtatttacct ttctgtcagc caaaacgtaa
      901 cccgtctgcc cggtggagct cgatcccggc aggacaagct cactttacag acctgtccgc
      961 agcgccctgc ctgccacgcc agaactcgca ttgcgggcat tcctgccccg ccggataatc
     1021 gacgccttct tcgtgagggc aaccgattat gccaccggca acggaaaccg tttttgcggc
     1081 agatctatca atcagtttga tgatggccga ttgaatggtg gcgtcctccc ggatgtcgcc
     1141 atccgtcctg aaacattccg tctgcggttc gctgttttcc tcttcgacaa aacggatcgc
     1201 caccttggtg gcaagcttgt cgtgggggcc gtaatacgcg atggcaatga ccgggtaacc
     1261 ctgaaatcct tttttggcct tcttggcaat tcgtttggtg gatttatcca gttccatgcg
     1321 caagatcatc tctgatgtgg ggtgggtgac gggaaatttt ggacaggtcc gtcaggcagg
     1381 aatgccgggc gacccgggtg tgacgatctc ggaatggagc caagtctagg ggatctcccg
     1441 ggtgaattac agcatcacgg acaaccaggc tgcacgcgca tgcgcgatga cgccaggcgc
     1501 aagactcact ggcactctcc cccaacaatc caggggatgc acacaaatcc cggatcaagg
     1561 aacttaatcc gcaattaatg acccaaggcc agccacagcg ccccatcctc cccgtagcat
     1621 tcaggctgca gaatgggcat aatcgcacga taacaacagc taaaaatctg ccaaaaacaa
     1681 cagattaggg ttgctacttg gcccaaaccc gggagtactc aagaccatgt cgaatcggga
     1741 cgcgattctc gcgctcaaag aatccctgtg cgaacagatc attggccagc agcggctggt
     1801 ggaccggctg ctgattgccc tgctggcgga tggccacctg ctggtggaag gcgcgcccgg
     1861 cctggccaag accaaggcca tcaagtccct ggcgcaaggc gtggaaggtg atttccatcg
     1921 catccagttc acccccgacc tgctccccgc cgacgtcacc ggcaccgaga tttaccgtcc
     1981 ccaggattcc agtttcgtgt tccagcccgg cccgattttt cacaatctgg tgctggcgga
     2041 cgaaatcaac cgcgccccgg ccaaggtgca atcagctctg ctggaagcca tgggcgaacg
     2101 gcagatcagc gtgggcaaat acacccacaa gctgccgcgc ctgttcctgg tcatggccac
     2161 acaaaacccc atcgagcagg aaggcaccta tccgctgccg gaagcccagc tcgatcgttt
     2221 cctgatgcat gtgaccattg gtttccccga tgcggcgtcg gaaaagaaaa tcctccagct
     2281 ggcgcgcatg gaagccctgg aaaagcaggc gccggatgac ggcgtgcccc cacccatcca
     2341 cattacccag cagcagattt tcgacgcccg cagtgaaatc ctgcagctgc acatgattcc
     2401 cgccatcgag gaatacatcg tgcagttcac catggccacc cgcaatccgc tggcctatgg
     2461 cgacgatctg tcgggctgga tcgaatacgg cgtcagcccg cgcggcacta tcgcactgga
     2521 ttcctgcgcc cgcgcccatg cctggctgaa caaccgcgat ttcgtcaccc ccgatgacgt
     2581 gcaggccgta gcccatgact gtctgcggca ccggctgggc ctgacatttg aagcggaagc
     2641 caagggcgtc actgccgatc acatcatcga cgaactgatc gatcgcgtct ccgcgccctg
     2701 aggtgcaccc catggcaagc gtgaacaagc tgctgcacga acgggccgat atcgcccagt
     2761 atcgcggcgt gtatgtgtcg atggacgagc tggtgtcgct gcagggggcc gagctgcgac
     2821 ttgacctgcg cggcaaacgc aaatccctgg cagggctggc gggccagcat cgatcctcgt
     2881 tccgtggccg tggcatggat ttcgacgaag tgcgcatcta c
//
LOCUS       DASUEG010000444         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_133604, whole genome shotgun sequence.
ACCESSION   DASUEG010000444 DASUEG010000000
VERSION     DASUEG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133604"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1061
                     /locus_tag="VFX11_08335"
     CDS             159..1061
                     /locus_tag="VFX11_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938666.1"
                     /translation="MTSVTLRDGEKLHVTCVGKGKPVMLLHGFGSRGAHWLHNVLPYT
                     RQFRFYLPDLRGFGNSHHANLDGLDVFATYANDVEDVLNHFELDQVILGGVSTGAYSC
                     LTYNQLYGFARVSKYLNIEHGPDSCHGDGQFNGIFREQQAELFAIFQDLLDLAQRSGP
                     VAYWELPAEHRLQFRDTVVRVLSQALHKGFARQVVMLAARLAEPLLTRYFMPVEKWQT
                     YLHVMQAFMNGRNTRLALANIKVPTTLMIGRQSQYFSVETQLEIAQYVPHAKVVIFER
                     AGHAVMVDQPIEFQREFGRFLFKA"
     gene            1441..2013
                     /locus_tag="VFX11_08340"
     CDS             1441..2013
                     /locus_tag="VFX11_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938667.1"
                     /translation="MGDPRFSLGDFRLHLDPEIEAQIELIQRGRLRGIMLQPNWSQFQ
                     KSDLDRMLQQPLPPAPPPAFPRGAGPATPRAAQVGDLLGAVWRLPFMQQAATRLADDA
                     SRRFQRDWGQATTGERALVITQAIVVGGGALTGVLTNSPTRVQVLNFLSGKDIPVPGL
                     PGLSVQLRHNSTLGDYGGILTFDLAPYMFR"
     gene            complement(2024..>2654)
                     /locus_tag="VFX11_08345"
     CDS             complement(2024..>2654)
                     /locus_tag="VFX11_08345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX4938668.1"
                     /translation="PVKSTAYIRIQDSESFCSAALIAPRLLLTARHCVQLPGTDQAMS
                     PDRLIVGFGDNALQSEDHGIVVQAIRLPAQSEFSRAEDLIGTDLALVQLNRAAPIPVM
                     AVAKFQQEAVTEQLTLIGYGVTRSGYYGLRHEIRVKVHAQDKTTLTFSGGGCRGDSGG
                     PLVNEQGELLAIVSLGTSRLCTLRQPRFGQRINAMQGFLEPFLRVSADD"
BASE COUNT          582 a          817 c          702 g          553 t
ORIGIN      
        1 ccccggcacc gtcgaaatcg tccaatcgct gaccgcatcc cagggataca cttggcccgg
       61 attctccccc ttcccgttcc cacggacagt acataccggc cacagatccg ctaccgtggc
      121 cgctcagatc agaacccagc agggattaac gaaaaacgat gaccagcgta acgctcagag
      181 acggggaaaa actgcacgta acctgcgttg gcaaaggcaa gccggtgatg ttgctgcatg
      241 gcttcgggtc gcggggagca cactggctgc acaatgtatt gccctacacc cgccagtttc
      301 gcttctacct gcccgatctg cgcggcttcg gcaactccca ccacgccaat ctggatggcc
      361 tggatgtctt cgccacctac gccaacgatg tcgaagacgt actgaatcac ttcgaactgg
      421 atcaggtcat cctgggtggc gtctccactg gcgcatacag ctgcctcacc tacaaccagc
      481 tgtacggatt cgcccgcgtc agcaaatacc tgaatatcga acacggccct gacagctgcc
      541 acggcgacgg ccagttcaac ggcatcttcc gggaacagca ggcggaactg tttgccatct
      601 ttcaggacct gctggacctg gcgcaacgct ccggccctgt cgcctattgg gagttaccgg
      661 cagagcaccg gcttcaattc cgcgataccg tggtgcgggt gctgagccag gccctgcaca
      721 agggctttgc ccgacaggtg gtgatgctgg cggcccgcct ggcggaaccg ctgctgaccc
      781 gctacttcat gccggtggaa aagtggcaaa cctacctgca cgttatgcag gccttcatga
      841 atggccgcaa tacccgcctg gcgctggcga atatcaaggt gcccaccacc ctcatgatcg
      901 gccggcagtc gcagtacttt tcggtggaaa cccagctgga aatcgcccag tacgtgcccc
      961 atgccaaagt ggttatattt gaacgggccg ggcatgcggt gatggtggac cagcccatcg
     1021 agttccagcg cgaatttggc aggttcctgt tcaaggcgta agcctgtcga caactccact
     1081 caaacatcct tcaaccactg cacaacatta atgaactgcc caaagtagac caatgccctc
     1141 tcccaacaat cctgcaaccg tttataggaa gggttgatag gctggctctt gaatacggtc
     1201 atgatttttt ttcagattcg ccaccgcggt tcacgttttt tgtgttgtcc tctattggag
     1261 tttgtaccta aattggcaat ccaaaacagg atttaatctc ggcagcatcc ggtacgattc
     1321 tatccgtctt actccaatag gctgttacag tggcatttgc gcgctcatgc cttacacatg
     1381 aacggctggc agacagatcg ataccgtcat tgaagcagcg aaccaaaagg agcacgttcc
     1441 atgggagatc ctcgattcag cctgggcgat tttcgactgc atctcgatcc cgaaattgaa
     1501 gcccagatcg aacttattca acgcggaaga ctgcggggaa tcatgcttca gcccaactgg
     1561 tctcagtttc agaaaagcga tctcgaccgg atgcttcagc aacccctgcc acctgcccca
     1621 ccgcccgctt ttccacgtgg cgccgggccg gcaactccac gcgcggcaca ggtcggagac
     1681 ctgctcggtg ctgtatggcg gctgccgttc atgcagcagg ccgcaacacg cctggcggat
     1741 gatgcgtcgc ggcgctttca gcgcgactgg ggccaggcca caacaggaga acgggcgctt
     1801 gtgatcaccc aggcgattgt ggtgggcgga ggggctctga ccggcgtgct cacgaacagt
     1861 ccgacacgcg ttcaggtgct gaactttctc tcagggaagg acattccggt accaggatta
     1921 ccggggctga gcgtacagct ccggcacaac agtacgctcg gtgactacgg tggaatactg
     1981 acgttcgatc tcgcccccta catgttccgg taaagcattt cattcaatca tctgcgctca
     2041 cgcgcaggaa cggttcgaga aagccctgca tggcgtttat ccgctggcca aaacgcggtt
     2101 gtcgcagggt gcacaaccgg ctggttccca ggctgacgat tgccagcaat tcaccctgct
     2161 cattcaccaa aggcccaccg gaatctcccc ggcatccacc accgctaaag gtcagtgtcg
     2221 tcttgtcctg ggcatgaacc ttcacccgaa tttcgtgacg caaaccataa tagccggagc
     2281 gggtaacacc gtacccgatc aatgtcagtt gttcggttac tgcctcttgc tggaatttcg
     2341 ccacagccat caccggaatc ggcgcagccc ggttcagctg taccagcgcc agatcggttc
     2401 cgatcaaatc ctccgcacgg gaaaattctg attgtgcggg tagcctgatt gcctgcacaa
     2461 caatgccatg atcctcactt tgcagtgcat tatcgccaaa gcccacgatc aaccgatccg
     2521 gtgacatcgc ctggtcggta cccggtagct gcacacaatg gcgtgcggtc aacaacagcc
     2581 ggggagcgat caatgcagca ctgcaaaaag actcgctgtc ttgaatgcgg atgtacgctg
     2641 tcgatttcac tggg
//
LOCUS       DASUEG010000445         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_580348, whole genome shotgun sequence.
ACCESSION   DASUEG010000445 DASUEG010000000
VERSION     DASUEG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580348"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFX11_08350"
     CDS             complement(<1..1007)
                     /locus_tag="VFX11_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX4938669.1"
                     /translation="MAIVGSVVVVGCGKIGSAIVKLLHAAGRFKLKVVEQHKPLLESV
                     AHLKGVETASFDVHDAALLRQAIQGWNVVVSACSYDVNPLIASVCAETGCSYFDLTED
                     VETTHRIRALAEQARPGQIFMPQCGLAPGFIGILAWDICRHFDKLDSVKMRVGALPKY
                     PTNRMMYNLTWSTDGLINEYCNLCEAIQDHQTMNLVALEGLEQISLDGVVYEAFNTSG
                     GLGTLCETLQGNVRELNYKTVRYRGHQYLMQFLIRELQLGDGHKRAMLKSILEEAVPM
                     TRQDVVLTFASVQGWRNGQFEQMTDARKIYHKKFHGENWSSIQLTTASSLCAVVDLFF
                     AG"
     gene            complement(1012..1821)
                     /locus_tag="VFX11_08355"
     CDS             complement(1012..1821)
                     /locus_tag="VFX11_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HEX4938670.1"
                     /translation="MDCSVFFQRLWEQYTAVTPQAQRIHDAVQARNGVVVNDHVAFRT
                     FRHSPLDIAHLEPVLAQLGYAPFERYRFVQKKLEAIAYRHPDPLQPKIFLSELLLDQL
                     SEAARNIILRRLGIGELPEPEDPSFFWQGILWPRISHAEYQQLLQESEYAAWLMVMGY
                     CANHFTISVNHLARTPSLDAVLDIVAELGLHLNESGGLIKGSAAVGLQQASTLADEME
                     YCFADGDVHPVKTCYYEFALRYPLSDGALYQGFVEQSADRIFESTFHQRTG"
     gene            complement(1951..2160)
                     /locus_tag="VFX11_08360"
     CDS             complement(1951..2160)
                     /locus_tag="VFX11_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX4938671.1"
                     /translation="MGKGMLMLGFLLVAAGLVILYAPGLLSWFGKLPGDIRVESGNGS
                     LFIPFTSMALVSLVATIVLTLLGRD"
BASE COUNT          507 a          709 c          597 g          443 t
ORIGIN      
        1 cccgcaaaaa acaggtccac cacggcacac agactggatg ccgtcgtcag ctggatcgaa
       61 ctccagttct cgccgtgaaa ttttttgtgg taaatcttgc gcgcatccgt catctgctcg
      121 aactggccat tgcgccagcc ctgcaccgag gcaaaggtca gcaccacatc ctgccgcgtc
      181 atcggcacgg cctcttccag aatggacttc agcatcgcgc gtttgtgccc gtcgcccagc
      241 tgcagttcgc ggatcaggaa ctgcatcagg tactgatggc cgcgatagcg cacggttttg
      301 taattcagtt cgcgtacgtt cccctgcagg gtttcgcaca gggtgcccaa tccgcccgac
      361 gtgttgaagg cttcatacac cacgccatcg agggaaatct gttccagccc ttccagtgcc
      421 accagattca ttgtctggtg atcctggatc gcctcgcaca gattgcagta ttcgttgatc
      481 aggccgtcgg tggaccaggt gaggttgtac atcatgcggt tggtgggata cttgggcagc
      541 gcgcccaccc gcattttcac ggaatccagc ttgtcgaaat ggcggcagat atcccaggcc
      601 agaatgccga taaagcccgg agccagaccg cactggggca tgaaaatctg gcccggcctg
      661 gcctgctccg ccagcgcccg gatacggtgg gtggtttcca cgtcctcggt cagatcgaaa
      721 tagctgcaac cggtttcagc gcagacactg gcaatcagcg gattcacatc ataggaacag
      781 gccgacacca ccacattcca gccctgaatg gcctgccgca gcaatgcggc atcgtgcaca
      841 tcgaagctgg cggtctcgac acccttcagg tgcgcaacgg attccagcag tggcttatgc
      901 tgctccacca ctttcagttt gaaccgcccc gccgcatgca acagcttgac gatggcggaa
      961 ccgatcttgc cacaccccac cacgaccaca ctgccaacga tcgccatgat cctatcccgt
     1021 gcgctgatga aaagtggatt cgaaaatccg gtcggcgctt tgctcgacaa acccctgata
     1081 caaggcgcca tcacttaaag gatagcgcaa ggcgaattcg taatagcagg ttttgacggg
     1141 atgcacgtca ccatctgcga agcagtactc catttcatcc gccagggtgg acgcctgctg
     1201 caaccccacc gccgccgatc ccttgatcag cccaccgctt tcgttcaggt gcagccccag
     1261 ttccgcaaca atatccagca ccgcgtccag cgacggcgtg cgcgccagat gattcacact
     1321 gatggtgaaa tgattggcgc aataccccat caccatcagc caggcggcgt attcactttc
     1381 ctgcagcagc tgctggtatt cggcgtggga aatgcgcggc cacaggatgc cctgccagaa
     1441 aaatgaaggg tcctccggtt ccggcaactc gccaatgccc agccggcgca ggatgatatt
     1501 acgcgcggct tccgacagct ggtccagcag cagctcgctg aggaagattt tcggctgcag
     1561 cggatcggga tgacggtagg caatcgcctc cagttttttc tgcacaaagc ggtaacgctc
     1621 gaacggcgca taacccagtt gcgccagcac cggttccaga tgggcaatat ccagggggga
     1681 atgacggaag gtgcggaacg ccacatgatc attgaccacc acaccattgc gggcctgcac
     1741 ggcgtcatgg atgcgttggg cctgaggcgt cacggccgtg tactgctccc acaagcgctg
     1801 gaaaaacacc gaacaatcca tacgcgacct cctgcggcaa aacagctgcg caacagcctg
     1861 tttcttcaca ataggagtgg ccggaggatt ttagaaagac tcagatattg ccagcagtaa
     1921 ccggcttatg cgctgcaatg ctaaggcgga tcaatcccgc cccagcagcg tcagcacgat
     1981 ggtcgccacc aggctcacca gcgccatgga cgtgaacgga atgaacagcg agccattacc
     2041 cgactcgacc cggatatcgc ccggcagctt gccgaaccag ctcaacaggc ccggggcata
     2101 caaaattacc agcccggccg ccaccagcag aaaacccagc atcagcatgc ccttacccat
     2161 gttccacccc ctgcggtgtg cactggatgt ccgtatccgg ctgccaatcc tttatgacgt
     2221 tttcattaat ttaggaagat gattctaatt tttctg
//
LOCUS       DASUEG010000446         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_577492, whole genome shotgun sequence.
ACCESSION   DASUEG010000446 DASUEG010000000
VERSION     DASUEG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577492"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1319)
                     /locus_tag="VFX11_08365"
     CDS             complement(318..1319)
                     /locus_tag="VFX11_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4938672.1"
                     /translation="MPAPTVAFELYDAIYHYLNQLGHADEQLQKRIRVRSRRDFMLNG
                     RVPLTLYEQAFLAAEDITGDTSIGMRIGGAVLPSSIGVFYFLSTAGESLSQILQAINK
                     YFPLAFDFIQLDMSCDDEHFRIALHYVHNRRPNRHVVEHLMAHWYCVANRLAFDTTNV
                     PRTLYLKNPQTADAQLIADIFGTTPVLFEQPLDGFDLRLENLDFHAGKANPQLFNLSE
                     RRADNLLMRLRAHDRIAKEISSHVMDMLESGSPGIEDVAKRMNCSGRTLQRRLAERNL
                     NYQMLLDNIRKDVAIELLSTTALPITQIATRTGFADDSTFHRAFKRWTGASPGAYRS"
     gene            1668..>3679
                     /locus_tag="VFX11_08370"
     CDS             1668..>3679
                     /locus_tag="VFX11_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938673.1"
                     /translation="MKRTSVHSIHFSGFHFLPLLLALWLAACGGDYSGTGSDSESRAA
                     NPTDTNTNPTTPGENSDPQTAATILEAFFVARIEPHMGYCGTCHTPGSIADTEEGHGF
                     LLQPDLSHYASFQLAWETLGKGIVDNGLLKENADMAEPHTGGKTWPAGSQINADVATL
                     LLCWNEPAQCPLTADDDDSNTGGDDDDDDGNSGEDPDDGNTGGNTGNNNLPLLGSSHA
                     NHLWNQFCATASADALLPADPRSLIVPGVNQNRAVYFNAYYQDCHVNLPEAEKAPKTC
                     GEYLARVERGDYFANQRAAMTDLSISASSINNLWKRWGLKQRPDNFDQLLTERYGFNP
                     APYPNPYPLPGEDPVATNGGSGQLPMGLIQARDASGNYNGKVSMNCYICHGGQIGSEE
                     DGEGLGPIPGMGNTNTDMMVFMRDLSGGLVGGLLPFGLNNVRGTSNAVGAFDLLTMIW
                     DVDTLSLSPNPIKLPFSHSYHGNQDMPNWWNTSHRPRKFFDGGVSVDSTRIDMAAADQ
                     VNLLRSGAVRRAATELYDQDLQAYVDAQTSPPFPGEIDTALAEQGAIIFHNKNLWANG
                     ANANLPQPQGNGSCAGCHGAYSPRYVNDPAFLENPVLEGVAGYIVPLDLIMTDPARAL
                     SITAQVRGVFKSSWWGYPDGMPGYIPPGEKSGWDEYRDDMAWPIINP"
BASE COUNT          826 a         1092 c         1017 g          744 t
ORIGIN      
        1 ccagcactcc ccagaaattc gctttttatg cttactggtt atgagcgaaa cagcttgtaa
       61 caggtacccc ggttgacccc gcccgcgctc gggatcagga tgactgggcc gcaaaaacag
      121 acaaagacga taggaggtcg aagcttatga gaatttcact ggatactgac gtgcttttct
      181 ggcttacgtc gaaagatgaa gaaagaatga tgaatgcctt gcacgattat ctggataaca
      241 tgtatttcgc caactagata cagcaagcac cacaagaagc ccgctactgc gggcttcgct
      301 gtttttattg cccgcattca gctgcgatag gcgccgggcg atgcgccagt ccagcgcttg
      361 aaggcacgat ggaatgtgct gtcgtccgcg aagccggtac gggtcgcaat ttgcgtgatg
      421 ggcaacgcgg tggtggatag caattcgatc gccacatcct tgcgaatatt gtccagcaac
      481 atctggtaat tcagattgcg ctcggccagc cgccgctgca acgtacgtcc ggaacagttc
      541 atgcgcttcg ccacatcctc gataccgggc gagcccgact ccagcatgtc catcacatgc
      601 gaggatatct ccttcgcaat ccggtcatgg gcccgcaggc gcatcagcag attgtccgcg
      661 cgccgctctg acagattgaa caattgcgga ttcgccttcc cggcgtgaaa atccaggttc
      721 tccagcctca gatcaaatcc atccagcggc tgctcgaaca gcaccggcgt ggttccgaaa
      781 atatccgcaa tcaactgtgc gtcagccgtc tgcggatttt tcagataaag tgtgcgcggt
      841 acattggtcg tgtcgaatgc cagccggttg gccacgcaat accagtgcgc catcagatgt
      901 tccaccacgt ggcggttggg ccggcggtta tgcacataat gcagcgcaat gcggaaatgc
      961 tcatcatcgc aggacatatc cagctggata aaatcaaagg ccagcggaaa gtacttgttg
     1021 attgcctgca ggatctggct gaggctctcg cccgctgtcg acagaaaata aaacacgcct
     1081 atcgaggatg gcagcacggc accgccgatg cgcataccga tggaggtgtc acccgtgatg
     1141 tcctcggctg ccagaaatgc ctgttcatac agcgtcagcg gaacacggcc attgagcata
     1201 aaatcgcgac gggaccgcac gcggattcgc ttttgcaact gctcgtcggc atggcccagc
     1261 tgattcagat aatggtaaat cgcgtcgtac aattcgaatg cgacagtggg tgctggcatg
     1321 gtactttgtc tccctctacg gcgatcggaa aacaacggga tcgcccaata cttctttctc
     1381 aaaatcaatt tgattttgtt atcacttttc cggtttttca tgacatccgg atgcgactga
     1441 ttctgcctgt ccggctgccg tggacggccg ccggaactgg ttgagtatga cacgccagtg
     1501 tcgcgattgg ccagccctgg tattgaaagc gcgaacggcg tcaacgtacc ctgcatccaa
     1561 caacaaaacc aagaacggcg gccaatggct ttcgcgcaac ggctttttct gttacgcgca
     1621 ttttccgcgc tcacatcatc cacccgctcg tcatggaggc aggggacgtg aaacgcactt
     1681 ctgttcactc gatacatttc tcaggttttc atttcctgcc actgttgctg gcgttgtggc
     1741 tggcagcatg cggcggcgac tacagcggca ccggcagcga cagcgaatcc cgtgctgcca
     1801 atcccacgga taccaacacc aatccgacca caccgggtga aaactccgat ccgcaaacgg
     1861 ctgcaacgat actggaagca tttttcgttg cgcgcatcga accccacatg ggttactgcg
     1921 gaacgtgcca cacaccgggc agcattgctg acaccgagga aggccacggc ttcctgctgc
     1981 aaccggatct cagtcactac gccagtttcc agctggcgtg ggaaacactc ggcaagggaa
     2041 ttgtcgacaa cggactgctg aaggaaaacg ccgacatggc ggagcctcac accggcggca
     2101 aaacctggcc tgcaggcagc cagatcaacg ccgatgttgc aacgctgttg ctgtgctgga
     2161 atgaaccggc gcaatgtccg ctgaccgccg atgatgacga cagcaacact ggcggcgatg
     2221 atgacgatga cgacggcaat agcggcgaag atccggatga cggcaacacc ggtggcaata
     2281 ccggcaacaa caacctgccg ctgctgggca gctcccacgc caatcatctg tggaaccagt
     2341 tctgtgccac cgccagtgcc gacgcactgc tgccggccga tccacgttcg ctgattgtgc
     2401 ccggcgtcaa ccagaaccgc gctgtgtatt tcaacgcgta ctatcaggat tgccacgtca
     2461 acttgccgga agcagaaaag gcgccgaaaa cctgcggcga atatctggca cgggtggagc
     2521 gcggcgatta ctttgccaac cagcgcgccg ccatgacgga tctttcaatt tccgcatcca
     2581 gcatcaacaa tctgtggaaa cgctggggcc tgaaacagcg accggacaat ttcgaccagc
     2641 tgctgaccga gcgctacggt ttcaatcccg cgccgtatcc caatccctat ccgctgcccg
     2701 gtgaagatcc cgttgccacc aacggcggct ctggccagct gcccatgggc ctgatccagg
     2761 cgcgcgacgc cagcggcaac tacaacggca aagtgagcat gaactgctac atctgccacg
     2821 gtggccagat cggttccgaa gaagacggcg aaggcctggg cccgatcccc ggcatgggca
     2881 acaccaacac cgacatgatg gtcttcatgc gcgacctttc cggtggactg gtgggcggac
     2941 tgctgccgtt tggcctcaac aacgtgcgcg gcacgtccaa cgcagtgggt gcattcgatc
     3001 ttctcaccat gatctgggat gtggatacgc tgtcactgag cccgaacccg atcaagctgc
     3061 cgttctcgca ttcctatcac ggcaaccagg acatgccgaa ctggtggaac accagccatc
     3121 gtccacgcaa attctttgat ggcggcgtgt cggtggattc cacccgtatc gacatggcgg
     3181 cggccgatca ggtgaaccta ttgcgcagcg gtgcagtgcg tcgcgccgcc accgaactgt
     3241 atgaccagga tctgcaggcg tatgtggatg cacagacctc gccgcccttc ccgggagaaa
     3301 ttgacactgc actggcagaa cagggcgcca tcattttcca caacaagaac ctgtgggcca
     3361 atggtgccaa cgcaaacctg ccacaaccgc agggcaatgg ttcctgtgcc ggctgtcatg
     3421 gcgcctattc gccacgctat gtgaatgatc ccgcgttttt ggaaaatccg gtgctggaag
     3481 gcgtggccgg ctacatagtg ccactggatc tgattatgac cgacccggcg cgggcgctgt
     3541 cgatcactgc gcaggtgcgt ggtgtgttca agagttcctg gtggggctat cccgacggca
     3601 tgcccggcta tattccgccc ggtgaaaaat ccggttggga cgaatatcgc gatgacatgg
     3661 cgtggcccat catcaatcc
//
LOCUS       DASUEG010000447         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_987853, whole genome shotgun sequence.
ACCESSION   DASUEG010000447 DASUEG010000000
VERSION     DASUEG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987853"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..367)
                     /gene="grxC"
                     /locus_tag="VFX11_08375"
     CDS             complement(110..367)
                     /gene="grxC"
                     /locus_tag="VFX11_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX4938674.1"
                     /translation="MADIIMYSTPWCPYCIRARGLLKSKGVTFTDINVAGQPQLRQEM
                     EDRSGRQTVPQIWIGSLHVGGCDDLFLLERQGILDGLLARP"
     gene            complement(371..790)
                     /locus_tag="VFX11_08380"
     CDS             complement(371..790)
                     /locus_tag="VFX11_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4938675.1"
                     /translation="MDRLLEFVVNHPELVGPFVVLVVLFILLETRRGGKTVSTHQLTH
                     LVNREGALVLDVRDSKEFREGHITDARNIPFSSLKESLSQLEKFKDKPVVIVCKMGQH
                     AGAAGRILHENGFKDVRRLSGGLTTWSSEGLPLVKGK"
     gene            953..2503
                     /gene="gpmI"
                     /locus_tag="VFX11_08385"
     CDS             953..2503
                     /gene="gpmI"
                     /locus_tag="VFX11_08385"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX4938676.1"
                     /translation="MSQARKPHVLIILDGFGYREEPESNAIFHARKPVWDKLWRERPH
                     TLISGSGMDVGLPDGQMGNSEVGHMNLGAGRIVYQDFTRITKAILDGDFFTNPALTGA
                     VDKANAKGGAVHIMGLLSPGGVHSHEDHIVAMIELAAQRNAKAVYVHAFLDGRDMPPQ
                     SAQPSLEKVEAKLQELVASRKLDTGRIASISGRYFAMDRDNRWDRVEAAYNLLTLGEA
                     PYTAATPVQGLLAAYERGETDEFVKPTVIGAAPAPIKNGDSIVFMNFRADRARQITRA
                     FVNPAFDKFPRKTVPQLADFVMLTEYAADINATCAYPPAPLTNVFPDYLAQKGKTQLR
                     IAETEKYAHVTFFFSGGREEPYPGEDRILVPSPNVATYDQQPEMSAPEVTDKLVDAIK
                     SGLYDAVICNYANGDMVGHTGVFSAAVKAVETLDTCIGRLVEAVESAGGEMLITADHG
                     NCEQMEDPDSGQPHTAHTTDLVPLVYVGPRQLEWQVQDGKLSDIAPTLLKLMALPQPK
                     EMTGRALF"
     gene            2572..3759
                     /locus_tag="VFX11_08390"
     CDS             2572..3759
                     /locus_tag="VFX11_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4938677.1"
                     /translation="MIPTRVPPALTHFSRLLIASLLVACVLVCAPVRASQQPDPVATQ
                     KELKQVQEKLKQLQRKIKSTQGQRSAAEKELKKTEQAIGAAKTRLREVNTQQNDAQVE
                     IARLEAEQQRLAEEKKSQQAAIKASVVAAHRSGRQEYVKMLLNQEDPQQLSRHLKFYE
                     YFRAARLQKIDAFNQTLAEIAHNQEQITARVAELDALRTELDLQQQKLQEAQAQRKAM
                     LAKLDASLQSSAGQVKQLQANQSNLEKVLKAVRESMADLPRHMGSGPFGKLQGQLRWP
                     AQGRLLRSFGSYREEGALRWNGVLIGASEGSPVQAVAHGRVVFADWMRGFGNLIIIDH
                     GAGYLSLYGQNESLHKTPGDWVNAGDLIASSGSSGGQNQAGLYFEIRHRGKPVNPALW
                     CKS"
BASE COUNT          888 a         1336 c         1160 g          643 t
ORIGIN      
        1 tgtatcgcgg attgccggtc cgccattgaa caaggcaatt gttgcaataa caccgctgca
       61 acaggacggc tggaacaaaa tcggctggaa gcaaaaaaac gcgcgtgaat cagggccggg
      121 ccagcagccc gtccagaatg ccctgccgct ccagcaggaa cagatcgtcg cacccgccca
      181 catgcaggct gccgatccag atctgcggca ctgtctggcg gccgctgcgg tcttccattt
      241 cctgacgcag ctgcggctgc cctgccacgt tgatatcggt gaaggtgacg cccttggatt
      301 tcagcaatcc cctcgcccgg atgcagtagg ggcaccaggg cgtggaatac atgatgatat
      361 ccgccatcgc ttacttgcct ttcaccagcg gcaacccttc cgaactccag gtggtgaggc
      421 cgccactcag gcgccgcaca tccttgaatc cgttttcatg caggatgcgc cccgccgcgc
      481 cagcgtgctg gcccatcttg cacacgatta ccaccggctt gtccttgaac ttctccagct
      541 gggacaggct ttccttcagc gagctgaacg gaatgttgcg ggcatcggta atgtggcctt
      601 cgcgaaattc cttgctgtcg cgcacatcca gcaccagggc gccttccctg ttcaccagat
      661 gggtgagttg atgagtggaa accgttttgc cgccgcgccg ggtttccagc aggatgaaca
      721 gcaccaccag gacgacgaaa gggcccacca gttccggatg gttgacgacg aattcgagca
      781 gacgatccat tgaaattccc aaaaattcag tgacttgcag gaagcggcag caggccgccg
      841 cgaaagagag gctgagtata cccgaaaccg ccggcgctgc cgatcccggc caaattcggg
      901 taaactgtgc gcactctccc tgtaagcaac cactaacgtc gtagcagtga ccatgagcca
      961 agcacgcaag ccgcacgtat tgatcatcct ggatggattt ggttatcgcg aagaaccgga
     1021 atccaacgcc attttccacg cccgcaaacc cgtctgggac aagctgtggc gggaacggcc
     1081 ccacaccctg atctccggct ccggcatgga cgtgggcctg cccgatggcc agatgggcaa
     1141 ctcggaagtg ggccacatga acctgggtgc cggccgcatc gtgtatcagg atttcacccg
     1201 catcaccaag gctatcctcg acggcgactt ctttaccaac ccggcactca ctggcgcggt
     1261 ggacaaggcc aacgccaaag gcggtgccgt acacatcatg ggcctgctct cccccggcgg
     1321 cgtgcacagc catgaagacc acatcgtcgc catgatcgaa ctggccgccc agcgcaacgc
     1381 caaagccgtg tatgtgcatg cgtttctgga tggccgcgac atgccgccgc agtcggccca
     1441 gcccagcctg gaaaaagtcg aagccaaact gcaggaactg gttgccagcc ggaaactgga
     1501 taccggccgt atcgcctcca tcagcggccg ctacttcgcc atggaccgcg acaaccgctg
     1561 ggaccgggtg gaagccgcct acaacctgct gaccctgggc gaagcaccct acactgccgc
     1621 cactcccgtg cagggtctgc tggccgccta tgaacgcggc gagaccgacg aattcgtcaa
     1681 acccaccgta atcggcgccg cccctgctcc gatcaagaat ggcgacagta tcgtgttcat
     1741 gaacttccgc gccgaccgtg cccgccagat cacccgcgcc ttcgttaatc cggccttcga
     1801 caaattcccg cgcaagaccg tgccgcaact ggccgatttc gtcatgctca ccgagtacgc
     1861 cgccgacatc aacgccacct gcgcctatcc gccggcaccg ctcaccaacg tgttccccga
     1921 ctatctggcg caaaaaggca aaacccagct gcgcatcgcc gaaactgaaa aatacgccca
     1981 cgtgacgttc tttttcagcg gcggccgcga agaaccctat cccggcgaag accgcatcct
     2041 ggtgccctct cccaatgtcg ccacctacga ccagcagccg gaaatgagcg cgcccgaagt
     2101 caccgacaag ctggtggacg cgatcaaaag cggtctgtac gacgcggtga tctgcaacta
     2161 cgccaacggc gacatggtgg gccacaccgg cgtattcagc gcggccgtaa aggcggtgga
     2221 aaccctggac acctgcattg gccggctggt ggaagcggtg gaaagcgcag gcggcgagat
     2281 gctgatcacc gccgatcacg gcaactgcga acagatggaa gatcccgaca gcggccagcc
     2341 ccacaccgcc cacaccacgg atctggtgcc gctggtgtac gtgggtccgc gccagctgga
     2401 atggcaggtg caggacggca agctgtcgga catcgcgccc accctgctca agctgatggc
     2461 gctgccccaa ccgaaggaaa tgaccggcag ggcactgttc tgataccgcc acggcgggcc
     2521 ggcttccggc ccgcacctgt tcgaccagcc aggggcgcca ctcccacacc catgatcccg
     2581 acccgcgttc cacccgcact gacccacttc tcgcgcctgc tgatcgccag cctgctggtg
     2641 gcgtgcgttc tcgtgtgcgc accggtgcgt gccagccagc aacctgatcc ggtcgccacg
     2701 caaaaggaac tcaagcaggt acaggaaaag ctgaaacagc tgcagcggaa aataaaatcc
     2761 acccagggcc agcgcagcgc cgccgaaaaa gaactgaaaa aaaccgagca ggccattggt
     2821 gccgccaaaa cgcgactgcg ggaagtgaac acccagcaaa acgacgccca ggttgaaatt
     2881 gcccggctgg aagcggaaca gcagcgcctg gcagaggaaa agaaaagcca gcaggccgcc
     2941 atcaaggcca gcgtggtggc ggcccaccgc agcggtcggc aggaatacgt gaaaatgctg
     3001 ctgaaccagg aagatccgca gcaactgtcg cgacacctga aattttacga gtacttccgc
     3061 gccgcccgcc tgcaaaaaat cgacgcgttc aatcagactc tggcggaaat tgcccacaat
     3121 caggaacaaa tcaccgcccg cgtggccgaa ctggatgccc tgcgcacgga actggatctg
     3181 cagcagcaga aactgcagga agcccaggcg cagcgcaagg cgatgctggc gaagctggac
     3241 gcgtccttgc aaagcagcgc cggccaggtg aaacagttgc aggccaacca gagcaatctg
     3301 gaaaaagtat tgaaagcggt acgcgaaagc atggccgatc tgccccgcca catgggcagt
     3361 ggccccttcg gcaaactgca gggacagttg cgctggcccg cccaggggcg cctgctgcgc
     3421 agtttcggca gctaccgcga ggaaggcgcc ctgcgctgga acggcgtgct gatcggcgcc
     3481 agcgaaggca gcccggtgca ggcagtggcc catggccggg tagtgttcgc cgactggatg
     3541 cgcggctttg gcaacctgat catcatcgat cacggcgccg gctacctgag cctgtacggc
     3601 cagaacgaat ccctgcacaa aacgccgggc gactgggtca atgccggcga tctgatcgcc
     3661 agcagcgggt ccagcggcgg ccagaaccag gccggcctgt atttcgaaat ccgccatcgc
     3721 ggcaagccgg tgaaccccgc gctctggtgc aagagctgaa cagccgggcc actttccgga
     3781 ctgctgccgc ggtttccgaa aattatcaac aacataatgc gactgccgcc atttcaggct
     3841 aggctaatca cattcaccgc acattgagga cacatcgatg ggttcgcgtt tcccttctgc
     3901 ctggcggcac cgggtactgc tgccgctgct ggcccctctg gccctgattc cagcggttct
     3961 tcccctgcag gcactggcgg aagacaagcc ggccgctgct ccggccaaac cggcagcaac
     4021 tgccccg
//
LOCUS       DASUEG010000448         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1111527, whole genome shotgun sequence.
ACCESSION   DASUEG010000448 DASUEG010000000
VERSION     DASUEG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111527"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..>2386)
                     /locus_tag="VFX11_08395"
     CDS             complement(167..>2386)
                     /locus_tag="VFX11_08395"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4938678.1"
                     /translation="PREQVLGNWQSGLQALGVNVRYGAEVKGITGNQGAFELKLKNGA
                     AVRAQHVVLGIGLQGNPRLLGVAGEGPDCVQYTLPDASAHRGETIVVVGAGDAAIENA
                     VALSAQNTVYLINRRDEFARAKEGNLALVLDAIDRGRIRCYYSSAPLRIDATPDAAKP
                     FQFTIKTRDGEAAIPCHRIIARLGAIPPRDFVESCGIRFPNDNPNAVPELSAKYESNV
                     PGLYVVGALAGCPLIKQAMNQGFEVVEHILGNPVQPADHPLLEQRLAVLPFGLEVDET
                     LDMFRSRIPMFRELTALQFRELMLDSKVIALGKNLKVGDALFKRGDYTNTFYTIVDGE
                     VLADVDEAGTQKRTLGSGQFFGEMSLLSGQRRDTSVYAGRDCVLVETPRRTMVKLLNS
                     NESVRRGLDEVFIMRSLQANFAPDASFDQLAPIARKCEFKTFNRGDFLFSEGDSGETM
                     FLIRSGSVILSRTSDGRELVFAQVPSGQYVGEAALMGNPVRMASARAAVFTETITIHR
                     DSFRALISLAPALVNTIQERAKVRLVQDVSVEADPGGGAVMAFLMEQGLGESTDVLVI
                     HEDLCVGCDNCEVACAETHGGVSRLDRAGGASFASVHVPIACRHCEFPHCMKECPPDA
                     IHRAPTGEVFIDNTCIGCENCVRNCPYGVIKMAYPAPKKPGLFSWLLFGAGAGPGQHH
                     DSSHSDDKSALKKAVKCDACKDLPQGPACVRACPTGAAVRLEPQRLSDLISRQKGGL"
BASE COUNT          460 a          827 c          673 g          426 t
ORIGIN      
        1 gtgcccagcg tgtaaccctg ccaggtgccc ccgttggccg gctgttcata attcggaaac
       61 agatataaaa gccaggacgc caatcccagc gccagcgccc aacaggcgta gtgaaaattg
      121 cggtaggcca gaatcgtctg atgaaaaatc ttcggcgcca cccgcatcag agcccaccct
      181 tctgccgcga aatcagatcg gacaaccgct gcggttccag ccgcaccgcc gcgcccgtcg
      241 gacacgcacg cacacacgca ggcccttgcg gcagatcctt gcaggcgtcg cacttgaccg
      301 cttttttcag cgcgctttta tcgtcgctgt gactgctgtc gtgatgctgg ccggggccgg
      361 cgcccgcacc aaacagcagc cagctgaaca gacccggttt tttcggcgcc ggatacgcca
      421 tcttgatcac gccgtaagga caattacgca cgcagttttc acagccgatg caggtgttgt
      481 cgataaacac ctcgccggtc ggcgcacggt gaatcgcatc cggaggacat tccttcatgc
      541 agtggggaaa ctcgcaatgc cgacacgcaa tcggcacatg caccgacgcg aacgacgccc
      601 caccggcccg gtccagccgc gacacgccgc cgtgggtttc agcgcaggcc acttcgcaat
      661 tgtcgcagcc cacgcacagg tcctcgtgaa tcaccagcac gtcggtggat tcccccaggc
      721 cctgctccat caggaacgcc atcacggcac caccgccggg atcggcttcc acgctcacgt
      781 cctgcaccag gcgtactttg gcgcgctcct ggatcgtgtt caccagcgca ggcgccagac
      841 taatcagcgc acgaaaactg tcgcggtgaa tggtgatggt ttcggtgaac accgccgccc
      901 gcgccgacgc catccgcacc gggttgccca tcagcgccgc ctcgcccacg tactgtcccg
      961 acggcacctg cgcaaacacc agctcgcggc catcactggt gcgcgacaga atcaccgagc
     1021 cgctgcggat cagaaacatg gtttcgccgc tgtcaccctc gctaaacaga aaatcgccgc
     1081 gattgaaggt tttgaattcg cacttgcgcg caatcggcgc cagctggtcg aaactcgcat
     1141 cgggcgcgaa gttggcctgc agcgagcgca tgatgaacac ctcatccagc ccgcgccgca
     1201 cgctttcatt cgagttgagc agtttcacca tggtgcggcg cggcgtttca accagcacgc
     1261 aatcgcgtcc ggcatacacg ctggtatcgc ggcgctggcc cgacagcaga ctcatctcgc
     1321 cgaaaaactg gccgctgccc agcgtgcgtt tctgggtgcc ggcctcatcc acatccgcca
     1381 gcacttcacc atcgacgatg gtgtaaaacg tattggtgta atcaccgcgc ttgaacaggg
     1441 catcacccac tttcagattt ttccccagcg cgatcacctt gctgtccagc atcagctcgc
     1501 gaaactgcaa cgccgtcagc tcgcggaaca tgggaatgcg gctgcggaac atgtccaggg
     1561 tctcatccac ttccagcccg aatggcagca cggccaggcg ctgttccagc aacggatggt
     1621 ccgccggctg caccggattg cccagaatgt gctcgacgac ctcgaagccc tggttcatcg
     1681 cctgcttgat cagcgggcag cccgccagcg cgcccaccac atacaaaccc ggcacgttgc
     1741 tttcgtattt cgccgacagt tccggcacgg cgttgggatt gtcgttggga aaacggatgc
     1801 cgcaggattc gacaaaatcg cggggcggaa tggcgcccag ccgcgcgatg atgcgatggc
     1861 agggaatcgc agcctcgccg tcgcgggtct tgatggtgaa ctggaacggt ttggcggcat
     1921 ccggtgtggc gtcgatcctg aggggcgcac tgctgtaata gcaacggatg cggccacggt
     1981 cgatggcgtc cagcaccagc gccaggttgc cttccttggc gcgggcaaat tcgtcgcgcc
     2041 ggttgatcag atacacggtg ttctgcgccg acagcgccac cgcgttttca atggccgcgt
     2101 caccggcgcc cactaccacg atggtctcac cccggtgggc cgacgcatcc ggcagggtgt
     2161 actgcacgca gtccggccct tcccccgcca cgcccagcag gcgaggattg ccctgcagac
     2221 cgatccccag caccacatgc tgcgcccgca ccgcggcacc gtttttcagt ttgagttcaa
     2281 acgcaccctg gtttcccgtg atgcccttca cttccgcgcc gtaacgcaca ttcacgccca
     2341 acgcctgcag gccggactgc cagttgccca gcacctgctc gcgcgg
//
LOCUS       DASUEG010000449         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1017309, whole genome shotgun sequence.
ACCESSION   DASUEG010000449 DASUEG010000000
VERSION     DASUEG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017309"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFX11_08400"
     CDS             <1..312
                     /locus_tag="VFX11_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938679.1"
                     /translation="LNLAVDAAKEALHIVAAKFGKRQNWTERLFSLFWVKMALPVMRF
                     LSPMDAESFVKFHFVKTAPQTRMMLKTFIDEGHKLGQPTLHLEELLDKLPDEPVLKKA
                     A"
     gene            complement(389..1369)
                     /locus_tag="VFX11_08405"
     CDS             complement(389..1369)
                     /locus_tag="VFX11_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4938680.1"
                     /translation="MTTIGEWWMWALFAGIVLIMLLIDLFLVGGGKQHRVPVKEAAIW
                     SVIWISVSFLFAGGLWWYLDAYHSRELANEKTLEYITGYLIEKSLAVDNIFVWIMLFS
                     FFAIPLELQKRVLLLGVFGALVMRTVMILAGAWLIARFHWILYGFGAFLLITGIKMYW
                     FADQKPDLEQNPLIRWLRAHMNITTELHGEKFFVVKQGVRYATPLFLALVLVEMSDLI
                     FAVDSIPAIFAITTDPFIVLTSNIFAILGLRAMYFLLADMADRFSLLKYGLAIVLMFI
                     GVKMLLIDLYKIPVGFSLAIVGVIIATSVGLSLVKARKVAAAGNSLPPQA"
     gene            complement(1738..>2018)
                     /locus_tag="VFX11_08410"
     CDS             complement(1738..>2018)
                     /locus_tag="VFX11_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485945.1"
                     /note="catalyzes the phosphorylation of protein substrates
                     at serine and threonine residues in vitro; specific
                     substrate in vivo has not been identified yet; plays a
                     role in long-term cell survival and expression of surface
                     appendages; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stress response kinase A"
                     /protein_id="HEX4938681.1"
                     /translation="LAGSRIEQRAQFSELLEGYEEFNDFNRRELQLIEPLRSMRMVHY
                     AGWLARRWSDPAFPANFPWFASAHYWQSHVNSLQEQLMVLDEEPFQLY"
BASE COUNT          451 a          552 c          617 g          398 t
ORIGIN      
        1 ctgaatctgg cggtagacgc ggccaaggaa gcgttgcaca tcgtggcggc gaaattcggc
       61 aagcggcaga actggaccga gcgtcttttc agcctgttct gggtgaagat ggcgttgccg
      121 gtgatgcggt tcctgtcacc gatggacgca gaatctttcg tgaaatttca cttcgtgaaa
      181 acagcgccgc aaactcgcat gatgttgaag actttcatcg atgaaggtca caagctgggg
      241 cagccgacgc tgcatctgga agagttgctg gacaagctgc ccgacgagcc ggttctgaaa
      301 aaggcggcct gaacggcgga ttgtccgcat cgcacgcaat gaaaaggtga cgttttccac
      361 gtcacctttt tttgttgcag aatgggcgtt acgcttgcgg cggcagactg ttgcccgcag
      421 ccgcaacttt gcgcgcttta accagcgata atcccaccga tgttgcgatg atcacgccca
      481 cgatagccag cgagaagccc accggaatct tgtacagatc gatcagcagc atttttaccc
      541 cgataaacat cagcacgatc gccaggccgt atttcagcag cgagaaacgg tccgccatgt
      601 ccgccagcag gaagtacatt gcgcgcaggc cgaggatggc gaaaatattc gacgtcaaca
      661 cgatgaacgg gtcggtggtg atggcgaaaa tggcgggtat tgaatccacc gcgaagatca
      721 ggtcggacat ttccaccagc accagggcga ggaacagcgg agtggcgtaa cgcacgccct
      781 gtttcaccac gaagaatttc tcgccgtgca gttcggtggt gatgttcatg tgcgcgcgca
      841 gccagcggat caggggattt tgctccaggt ccggtttctg atcggcgaac cagtacatct
      901 tgatgccggt gatgagcagg aaggcaccga agccatagag aatccagtgg aaacgggcaa
      961 tcagccaggc acctgccagg atcatcacgg tgcgcatcac cagcgcgcca aacacaccca
     1021 gcagcagcac ccgtttctgc agttccagcg gaatggcaaa aaacgagaac agcatgatcc
     1081 agacgaaaat gttgtctact gccagggatt tttcgatcag gtagccggtg atgtattcca
     1141 gcgttttctc gttggccagt tcccggctgt ggtaggcgtc gagataccac cacaggccgc
     1201 cagcgaacag gaaggacacg ctgatccaga tcacggacca gattgccgcc tctttcaccg
     1261 gtacccgatg ctgtttgccg ccgcccacca ggaacaggtc gatcagcaac atgatcagta
     1321 cgatgcccgc gaacagggcc cacatccacc attcgccgat tgtggtcatg agcggccctc
     1381 ctggctaggg actgtggacg gtccggacgg gattctcagg ggtgcgggta tgcgggcaag
     1441 agtgggtggt ggcgaaagag atcgccagag gcgggttgcc tgagtgcaga gaaaggtgag
     1501 gaacatgagg gttggtctcc ggtattgcgc gtgttcaaga ccaacgcaat gaccggacgc
     1561 gcgaaacctt tgtcattgcg acaaaggtct cgttcacagg cctgggcctg cccgtggtac
     1621 cggggcctgg tgcggcaagc gcacggcccg tgatgacgat agccacggcg ggaactactc
     1681 cccttcagtg aaaccagtgt atttgtccga ctggttttgt caactactgc tggtgcatca
     1741 gtaaagttgg aagggttctt cgtccagcac catcagctgt tcctgcaggc tgttcacgtg
     1801 gctctgccag tagtgggcgg aagcgaacca gggaaagttg gcgggaaagg ccgggtcgct
     1861 ccagcgccgg gccagccagc cggcatagtg caccatgcgc atggaccgta gcggctcgat
     1921 cagttgcagc tcgcgccggt tgaagtcgtt gaattcctca tagccttcca gcagctcgct
     1981 gaactgggcg cgttgttcga tgcggctgcc cgccagca
//
LOCUS       DASUEG010000450         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_27774, whole genome shotgun sequence.
ACCESSION   DASUEG010000450 DASUEG010000000
VERSION     DASUEG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27774"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFX11_08415"
     CDS             <1..704
                     /locus_tag="VFX11_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938682.1"
                     /translation="DSMKLRLRTGLVSSIAPTELNNDDLVALPTGEKIGPIRTTTQLD
                     FTLYLVKLPILQLSLQIHHYPKAVMYDVRGIIPEVRRYLLKDPSIAMTLDANRLLGST
                     IRMANGPKEPGVVDGKIDALEEQMRQIHFTPQDNWYWVSTKRNLDIVAYLDYLGEFNE
                     PISPVIDDDLTYEDPPEVFPGQLPNVGYRIDSFPMKGFLGFVVSIYVSDGLEGDPEVF
                     TRQLRTIPELQVRPL"
     gene            complement(848..1357)
                     /locus_tag="VFX11_08420"
     CDS             complement(848..1357)
                     /locus_tag="VFX11_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938683.1"
                     /translation="MKTTKMIGFSLLVAMSGVSVALADSTIVKVPRENGAVHQEFKNL
                     LNDTISKFRSGIGRIEMKGGAGKEMCAANFYTTNETTFVTLDVNGGKYYTEFYIDHPH
                     QSFKQILFQNLIMSDENVELKVVERDGGYSIVTDGKTLTLTSKTSQGQSPACEFSLAQ
                     AMLHEGETE"
     gene            complement(1370..>2964)
                     /locus_tag="VFX11_08425"
     CDS             complement(1370..>2964)
                     /locus_tag="VFX11_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938684.1"
                     /translation="TSFRSSGNVRAASGNNAVAALVLALAALLSTPAGAVTKAQLDTS
                     AAQVQSLNKSIAAAESRVATLDRDLRALDRQIQDKRKLVRDERNAGRKQVFDARRDLK
                     KQSFAIESLEKDIALVDFDIDVVRRDIERDKLRYAELNVLKQSMEEGEFKKRQAEYQR
                     QLDQLAEKKRPMAQNLEQAKAELAAMQSELDAVDGGNDDSSLDKDSRIAALVQKRDRT
                     SAEIGALNNQIRNDRNRLAQQQGNYQKLAAQFKREQASAQQTRVTSAVPAPQPVVTPP
                     PVAPAPAAATARPVLDRTDYTSYVFVISGDQEPDIEQTLHLKNWVESYGAKYIEARWN
                     GFNSNGGPQSTAGFQEAFRGYIRQIPKEAKLVLIGHGLGGGAAIEAATRVAFSEARTI
                     DFLAVLDPIGEQNLRANIVYDTSGSCTRPDPKDEMTNSDYVECIKSAKKRLITANIKH
                     FYNRWQKDANGPLDFQRQIPSLDTNGKVVSVPTATGRFEIADSIDSDQKRLYFAGDKN
                     AHKLLLAEEAKQLPKLLVQHLR"
BASE COUNT          528 a          860 c          873 g          703 t
ORIGIN      
        1 tggactccat gaaactgcgc ctgagaaccg ggctggtgtc atccattgca cccaccgaac
       61 tcaataacga cgatctggtg gccctgccca ccggcgagaa gatcggcccc atccgcacca
      121 ccacccagct cgatttcacc ctgtacctgg tcaagctgcc gatcctgcag ctgagcctgc
      181 agattcacca ttatcccaaa gcggtcatgt acgacgtgcg tggcatcatc cctgaagtcc
      241 gccgttatct gctgaaagat cccagcatcg ccatgacgct ggatgccaac cgtctgctgg
      301 gttccacgat tcgcatggca aacggtccca aggagccggg tgtggtggac ggcaagatcg
      361 acgcgctgga agagcagatg cgccagattc atttcacgcc gcaggacaac tggtactggg
      421 tcagcaccaa gcgcaatctc gatatagtgg cgtacctgga ttatctgggt gaattcaacg
      481 aaccgatctc gccggtaatc gatgacgacc tgacctatga agatccgccg gaagtattcc
      541 cgggccagct gccaaacgtg ggctaccgta tcgacagttt ccccatgaag ggttttctcg
      601 ggtttgtggt gagcatctat gtcagcgacg gcctggaagg cgatccggag gtgttcaccc
      661 ggcagctgcg caccattcca gaactgcagg tgcggccgtt gtgattccgg cttgccgggg
      721 cgccgctgcg caggcgctcc ggtaatgttc tagtcattga tcagcgtatt gtgggcaaca
      781 aaaaaccggc gaggagcgtt cgcgtcaccc cacgccggtc ggagaaaaac gtcagtttcc
      841 cttctgtcta ttcggtttca ccttcatgca gcatggcctg ggccagcgag aattcgcagg
      901 ccgggctctg accctggctg gtcttgctgg tgagggtcag cgtcttgcca tcggtcacga
      961 tggaatagcc gccatcccgc tccaccactt tcagctccac gttctcgtcg ctcatgatca
     1021 ggttctggaa caggatctgc ttgaacgact ggtgcgggtg atcaatgtag aattcggtgt
     1081 aatacttgcc gccgttcacg tccagcgtga cgaaggtggt ttcgttggtg gtgtagaaat
     1141 tggcagcgca catttccttg cctgcacccc ctttcatttc gatacggcca ataccggagc
     1201 gaaatttgct gatggtgtca ttcagcagat tcttgaattc ctggtgcaca gcgccgttct
     1261 cacgcggcac tttcacgatg gtgctgtccg ccagcgcgac gctgacaccg gacatggcca
     1321 ccagcaggga gaaacctatc atttttgtag ttttcataac gctctcctgt caacgcaagt
     1381 gctgaaccag taacttgggc agttgcttgg cttcttccgc cagcaacaac ttgtgcgcat
     1441 tcttgtcgcc ggcgaagtac agccgcttct gatcggaatc gatactgtct gcaatctcga
     1501 accgtccggt agcggtgggt acactcacca ctttgccgtt cgtgtccagg ctgggaatct
     1561 ggcgctggaa atccagcggg ccgttggcgt ctttctgcca gcggttgtag aaatgcttga
     1621 tgttggcggt gatcaggcgc ttcttggcgg acttgatgca ctcgacataa tcggagttgg
     1681 tcatttcgtc cttggggtcc ggacgggtgc agctgccgct ggtgtcatac acgatgttcg
     1741 cccgcaggtt ctgctcgccg atggggtcca gtactgccag gaaatcgatg gtgcgggctt
     1801 cggaaaaggc cacccgggta gcagcttcaa tggccgctcc gccgcccagg ccgtgtccga
     1861 tcagcaccag cttggcttcc tttggaatct ggcggatgta gccgcggaat gcttcctgga
     1921 aaccggcggt gctttgtggt ccgccgttgc tgttgaatcc attccagcgc gcttcaatgt
     1981 atttcgcgcc ataggactcc acccagtttt tcaggtgcag ggtctgctcg atgtcgggtt
     2041 cctggtcgcc tgagatgaca aaaacatacg aggtgtaatc ggtccggtcc agcaccggcc
     2101 tggcagtcgc tgcggcaggt gccggcgcta cgggcggcgg tgtaaccacg ggttgcggag
     2161 caggcactgc ggaggtcacg cgggtttgct gggcgctggc ctgttcgcgc ttgaactgcg
     2221 cggccagctt ctgataattg ccctgctgct gggcaagacg attgcgatca ttgcggatct
     2281 ggttgttcag ggcgccgatt tcggcactgg tgcggtcacg cttctgcacc agagcggcaa
     2341 tacgggagtc tttgtccaga gaggagtcgt cgtttccgcc atccaccgca tccagctcac
     2401 tctgcatggc ggccagttca gccttggcct gttccaggtt ttgtgccatc ggccgtttct
     2461 tttcggccag ttgatccagc tggcgctggt attctgcctg gcgcttcttg aactcgcctt
     2521 cttccatgct ctgcttgagc acattcagtt cggcataacg caacttgtcg cgctcgatgt
     2581 cgcggcgcac cacgtcgatg tcgaaatcca ccagtgcgat gtctttttcc aggctttcga
     2641 tggcgaagga ttgttttttg agatcgcggc gtgcgtcgaa gacctgcttg cggccggcat
     2701 tgcgttcatc gcgaaccagt ttgcgcttgt cctggatctg gcggtccagc gcccgcaggt
     2761 cccgatccag cgtggctacg cggctttccg ctgcagcaat actcttgttg aggctttgca
     2821 cctgcgccgc gctggtgtcc agctgggctt tagtgacggc gcctgccggg gtggacagca
     2881 gggctgccag tgccagtacc agcgccgcga cagcgttgtt ccctgaagct gcccgtacgt
     2941 tccctgagga tctgaaggat gtca
//
LOCUS       DASUEG010000451         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1884493, whole genome shotgun sequence.
ACCESSION   DASUEG010000451 DASUEG010000000
VERSION     DASUEG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884493"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..669
                     /gene="ndhC"
                     /locus_tag="VFX11_08430"
     CDS             256..669
                     /gene="ndhC"
                     /locus_tag="VFX11_08430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX4938685.1"
                     /translation="MEPVIATSPPIWPLAAYFVATLLIVGVILILSHFLGQRRRNPAT
                     VEPFESGIVSVGTARYRVPIPFYLLAIFFVIFDLEAVFLFAWAIAFREAGWPGYIEAM
                     IFIGILGATLAYLWKLGALDWRTPRQKEQQSNYKR"
     gene            727..1383
                     /locus_tag="VFX11_08435"
     CDS             727..1383
                     /locus_tag="VFX11_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX4938686.1"
                     /translation="MSWSITRPSQSPVAGAVDETSGSNEFWESLRQNVVMGKLEELVS
                     FSQANSLWPFNFGLSCCYVEMATTFTSQFDIARFGAEVIRGSPRQADLIVISGTVFRK
                     MAPVLERLYEQLIEPRWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVQGCPPRPD
                     SLLEGLMLLQNVIKSQRRAISWVISQEGAVHTIDRPSQREARREERIHIRELRSPDEV
                     "
     gene            1390..>2103
                     /locus_tag="VFX11_08440"
     CDS             1390..>2103
                     /locus_tag="VFX11_08440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX4938687.1"
                     /translation="MVAETSHVASSGTHHWHGEAAHRIDYQKWLNQPIIAELLRHFGE
                     KAFAPQLTVDEIPTLWVPVTQAIALFAHLRQGVPAPYCMLYDLTVIDERLRNHREGQP
                     ASDFTVVYHLLSIERNAFLRIKIALLEPALSVPSVTGIWMNANWYEREAWDMFGIRFD
                     GHPNLRRILLPPTWQGHALRKDHPARATEMEPFSLSAERQDAEQEALRFNPDDWCMQR
                     AHDDTDFMFLNLGPNHPSVH"
BASE COUNT          454 a          534 c          594 g          521 t
ORIGIN      
        1 cgtttcgcta ttgatcaaat aaccagaaat ttggatggta gttaatgcgt gtgagtgtta
       61 cagatattcc cgctttacag taaaaaaagg tggttgacca attccccgtt aacgccataa
      121 tgaataagcg cgttgcaata gctgatatgg aatcatcagt atccatcctc atctatcgat
      181 ctacaaattg aacaatttgt aagaagtaga tttttacgac cccacagcat cgtaaataga
      241 ggatcaacgg gtgacatgga acctgtcata gcaacaagcc ctcccatctg gcccctcgcg
      301 gcttattttg tcgccacgct gctcattgtt ggcgtcatcc tgatcctgtc acattttctg
      361 gggcaaagac gccggaatcc tgccacggtc gaacctttcg agtccggtat cgtgtcggtg
      421 ggcacggccc gctatcgcgt ccccattccg ttttatctgc tggcgatctt cttcgttatt
      481 tttgatctgg aagccgtgtt cctgtttgcc tgggccattg cctttcgcga ggcgggctgg
      541 cctggctata tcgaggcgat gatattcatc ggcatattgg gcgcgacgtt ggcatacctg
      601 tggaaactgg gcgcgctgga ctggagaaca ccgcgacaaa aagagcaaca aagcaactat
      661 aagcgctgat atacaagcgt cgatacaaac gttccttttg ttgcaataaa tggggggtag
      721 atgagtatga gctggagcat taccaggcct tcgcaatcgc ctgtggcagg ggcagttgac
      781 gaaacttcag gcagcaatga attctgggaa tcgctgcgcc agaatgtcgt catgggcaag
      841 ctggaagagc tggtgtcctt tagccaggcc aattccctgt ggcccttcaa tttcggtctt
      901 tcctgttgtt atgtggaaat ggccacgacc tttaccagtc aattcgatat tgcgcgcttt
      961 ggtgccgagg tgattcgtgg atcgccccgg caagcagatc tcattgtgat ttccggtact
     1021 gtgtttcgca aaatggcgcc ggtgcttgag cgcttgtatg aacagctgat cgagccgcgc
     1081 tgggtgatca gcatgggttc ctgtgcgaat tccggtggca tgtatgacat ttacagtgtg
     1141 gtgcagggcg tcgataaatt cctgccggtc gacgtgtatg tgcaagggtg tccgccgcgc
     1201 ccggattcct tgctcgaagg gttgatgctg ctgcaaaacg tgatcaaatc gcagcggcgt
     1261 gccatcagct gggtgatcag tcaggagggc gcggttcata ccattgaccg tccgagtcag
     1321 cgcgaagccc gccgtgaaga gcgtattcat atacgcgaac tgcgctcgcc ggatgaggtg
     1381 tgacgcatca tggttgcgga aacgtcgcat gttgccagtt caggaaccca ccattggcat
     1441 ggtgaggctg cgcaccggat tgactaccag aagtggttaa accagcccat cattgcagaa
     1501 ttgttgcggc attttggcga gaaggcattc gcgccgcagc tgacggtaga cgaaattccg
     1561 acactctggg tgcctgtcac ccaggccatc gcgctgtttg cgcatctgcg gcagggtgtg
     1621 cccgcgccgt actgcatgct ctatgatttg acggtgattg acgaacgcct gcgcaatcac
     1681 cgggaaggtc agcctgccag cgatttcact gttgtctatc atttgttgtc gattgagagg
     1741 aatgcctttc tccgcataaa aatcgccttg ctggagccgg cattgtcggt gccttcagtc
     1801 accggcatct ggatgaatgc aaactggtat gagcgggaag cctgggacat gtttggcatc
     1861 cgctttgatg gacacccgaa tttgcggcgc attctgctgc caccgacgtg gcagggccat
     1921 gcactgagaa aagatcatcc cgcccgcgcc accgaaatgg agcctttcag tttgtcggcc
     1981 gaacgccagg atgcggagca ggaggcgttg cgcttcaatc ccgatgactg gtgcatgcag
     2041 cgcgcgcacg acgatacgga tttcatgttt ctgaatctgg ggccgaacca tcccagcgtg
     2101 cat
//
LOCUS       DASUEG010000452         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_598679, whole genome shotgun sequence.
ACCESSION   DASUEG010000452 DASUEG010000000
VERSION     DASUEG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598679"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..370)
                     /locus_tag="VFX11_08445"
     CDS             complement(125..370)
                     /locus_tag="VFX11_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX4938688.1"
                     /translation="MSIEKHNLVLEFPESRDAIHALKTGNQHFSRLFEQYDDVDHEVR
                     RIETGVQTTSDEYLEKKKKERLKLKDELYGMIKEFRK"
     gene            527..1291
                     /locus_tag="VFX11_08450"
     CDS             527..1291
                     /locus_tag="VFX11_08450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4938689.1"
                     /translation="MLDLIDTHCHLDIAEFDACRAELVDRCRAAGMRAILVPGVSESG
                     WARLRQVCDQYSLLRPAFGLHPCYAAGHRDEHVAALESWLAPDVIAVGEIGLDTFHGD
                     QDFARQLELFSAQVAVARNHRLPLILHARKTHDPILKVLRQQKFSHGGVVHAFSGSLQ
                     QAQYFNELGFAVGFGGGATYDRALKLRSILKTLSLDYVVLETDAPDIPPSFARGEDNT
                     PFNLFRIAEILADVLQMAPADLAARTTRTAQRIFGL"
     gene            1463..1708
                     /locus_tag="VFX11_08455"
     CDS             1463..1708
                     /locus_tag="VFX11_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938690.1"
                     /translation="MSRSVTGQLPEPAEVIVEKLKKLAVKHDIEFAGDVVRGYARGKG
                     FHIEYRVEGVSCTLTVTKKPLLVPWAMVESQLHKLFS"
     gene            1759..2220
                     /locus_tag="VFX11_08460"
     CDS             1759..2220
                     /locus_tag="VFX11_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4938691.1"
                     /translation="MKLVAKEKLLEAVGTEMGASEWFLIDQARINAFADATLDHQFIH
                     VNPEQAKHTPFGGTIAHGFLTLSLLPYLQATMPDLLVPAGLKMGMNYGFDKIRFLAPV
                     KVNSKVRAVAKLKDAVEKNPGQFLLTAEYTVEIEGQPKPALIADWMVMYFV"
     gene            complement(2350..4521)
                     /locus_tag="VFX11_08465"
     CDS             complement(2350..4521)
                     /locus_tag="VFX11_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938692.1"
                     /translation="MHFPSPVSRNVLPRLVVSSAIALLTACGGGGGSNGGSSSATPTP
                     VVQASTVSGTVTKGVISNGLVSVYALEDGTTARLLGSTRTDQTGHFSLNVTGYQGAVY
                     IEVTAATSGEPTLMTCDSATGCGDFSGSSNYDSNNNGSIDFGERFAVSGTFMLSSALP
                     SIQQSVSTSVSTLTHLAAQLAAQFPQGANEVSIAAALSQVQNLFGLDGSLTGTRLIDL
                     TNPTAVSGATANELRYALLSGALMGLTNDVAFGETLAAFTEEFIINQGQLVQQDGGAG
                     STSYLDLVEQALATAQHLNLGSFVTELQQLKSTLQVAENGSLTDSQPAPTAGGATAAK
                     IDAFVADLALWEGYLSLNPQQASFAQVVSAMGVSTGADLSNMLQAMAIAGQYGPIVAL
                     PDLALGAACDSLGNYLTRMTCRLLIAGKSLEDICEGTLNLVIFNRSLCDILNDLTLPL
                     GAGVYGHFALYDGVVRIYGDVQDTEVDLTFTRASRSSTRYGFAVSGTVESAHGLLEIN
                     SGNVEMLFTGGLDIKNLKLPENANGTIQVNYQQFATDEHPDSMSFAGQVNLQLDLSNV
                     RESDDSGETAYSGLDQIGIQLTAAGEFSSVDGDSFAGSLSISGGVDSNVQVRFETDLP
                     DYSDRAVVTMTSSPANLAAGLIDTIRIRWAGKQYDIENYFSSNGIRITNQDGVIMDLD
                     LSVEDGVNAGRILLNGTAFGSVSPLNGSLLIQLSNGSELVL"
     gene            complement(4815..6629)
                     /locus_tag="VFX11_08470"
     CDS             complement(4815..6629)
                     /locus_tag="VFX11_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4938693.1"
                     /translation="MQLRLALAFVFACLFFGKAQAVERFSPDGKWIFNDHITIPALLD
                     PFDPGSEIIQLPANIFVPSSADAGATFPAIIYISSWALYEHEYLDQAQLLADKGYVVL
                     SYTARGFHSSPGLINTAGEADVSDARSAIDYLLDPNNHLPVDPQRIGMGGVSYGAGIS
                     LLTALQDERVKAVTAMSGWGDLIESLWAGNTPNYTWLEILVGTSKPIPYVLKNRPDPV
                     IEHYYNNMKVHQDIAETKAWGLIRSPISYMEQAQARANPPALFMSNNMHDYLFQPDSA
                     VRMLSQYQGPWRLELNRGTHASGEAGGLLGDEDHRIWVHAAQWLDHYLKGEDNGIDER
                     KRVNTVVLNTRKWESYPDLPVHDQTLVYQLNPQAGTQRGSLDTDSTTDSEQVLGFDTT
                     DFITYTGWVTGALEHTGRILDLASVEDRHGLLFNSQTLTSPVRLRGEAKVKLWAHMED
                     KTQYFAYLFYLNPETGKANWVGHAPFSCHNSEGCGLHPDQPEQITLDFYWTAVDLPAG
                     SQVLLVIDGNDPDYWRYDTTPEQNTLVISPEYPASLELPVVSEAGTFDDPVKQATDSS
                     RSANGAGSDSHSFSGGALGWLGLLPMLVLAIRRRRRLN"
BASE COUNT         1346 a         1853 c         2009 g         1442 t
ORIGIN      
        1 tatcagcggc attgcctgcg agtgatgctg tcgccgtata agtgggtagt cctgtaaatg
       61 aaaaagagga gcttcggctc ctctttccat tttgatgcgg ggctgttggc cagaggcgga
      121 aattctactt gcggaattcc ttgatcatgc cataaagctc gtctttcagt ttcaggcgct
      181 cctttttctt cttctccagg tattcgtcgc tggtggtttg cacgccggtt tcgatgcggc
      241 gtacctcatg atccacgtcg tcatactgtt cgaacagccg ggaaaaatgc tgattgccgg
      301 ttttcaacgc gtgaatcgca tccctgcttt caggaaattc cagtaccaga ttgtgttttt
      361 caatgctcat cgatgagatc ccgtgtggtt gctgttaata aaccaacgtt atcccattgg
      421 ggggcggact ggattgatcc gtatcaaccc tgccatgatc agggtttctg ggggcggacg
      481 gttgcgttgt gtatactgca gaagcaagcc ttctggagcc attttcatgc tggatctgat
      541 cgatacccat tgccacctgg acattgccga gttcgatgct tgccgcgccg agctggtgga
      601 tcgttgcagg gccgccggta tgcgcgcgat tctggttccc ggtgtgagtg aatctggctg
      661 ggcccggctg cggcaggtgt gcgatcagta ttccctgttg cggcccgcct ttggcctgca
      721 tccctgctac gcagccggcc atcgtgacga acacgtggcg gcgctggaat cctggcttgc
      781 accggatgtc attgcagtgg gcgaaattgg tctcgataca ttccatggcg accaggattt
      841 tgcgcgccag ctggagctgt tttcggccca ggtggccgtt gcccgcaacc accgtctgcc
      901 gctgattctg catgcgcgca agacccacga cccgatcctg aaagttctgc gccagcagaa
      961 gttcagtcac ggcggcgtgg tacatgcgtt ctccggcagc ctgcagcagg cccaatactt
     1021 caacgaactg ggttttgccg tgggttttgg tggcggcgct acctatgacc gcgcgctgaa
     1081 actgcgctcc atactgaaaa ccctgtccct ggattacgtg gtgctggaaa ccgacgcgcc
     1141 ggatatcccc cccagctttg cgcggggcga ggacaataca cctttcaacc tgttccgcat
     1201 tgctgaaatc ctggcagatg tcctgcagat ggcaccggcg gacctggcag cgcgcacgac
     1261 ccgcaccgcg cagcgcatat ttggtctgta acggtcagga cggcgcggct gcgctcaatg
     1321 ccgcctcgtg tggcgcctgg ccgctgagcc cgccagaatt gcctctggcc gccccgggca
     1381 atcatgataa aatccgacct cccggccgat ctgtattttt gcgcgggcga gccggattcg
     1441 aattaactga aagggtgtgt ccatgtcacg ttctgtcacc gggcaattgc cggagccggc
     1501 cgaagtcata gtcgaaaagc tgaaaaaact ggctgtcaag catgacattg aattcgctgg
     1561 tgatgtggtg cgcggctatg cgcgcggcaa gggttttcat atcgagtacc gggtggaagg
     1621 tgtgagttgc acgctcaccg tcaccaaaaa gccgctgctg gtaccctggg cgatggtgga
     1681 atcccagctg cacaagctgt tcagttgaat ttattgtttt ttgaatttgt tttttgaccc
     1741 tgagggagtt gcaataacat gaaactggta gcgaaagaaa aactgctgga agcggtcggc
     1801 accgaaatgg gtgcgtccga gtggttcctg atcgatcagg cccgcatcaa tgcctttgcc
     1861 gatgccacgc tggatcatca attcattcac gtcaatccgg agcaggccaa acacaccccg
     1921 ttcgggggca ccatcgcgca cggctttctg acgttgtccc tgctgcctta cctgcaggcc
     1981 accatgcccg atctgctggt gccggcgggt ctgaagatgg gcatgaacta cggcttcgac
     2041 aagatccgtt ttctggcgcc ggtgaaagtg aactcgaagg tgcgcgcggt ggccaagctg
     2101 aaggatgccg tggaaaagaa tccgggccag ttcctgctga cggcggaata cacggtggaa
     2161 atcgaaggcc agcccaaacc ggcgctgatt gctgactgga tggtaatgta cttcgtgtaa
     2221 ggccgttgtg atcgcttgat ggcgtgtcgg gaagtgacac atcaagcgac agtgcatata
     2281 aaatcagtgc acaaaaaaaa gggagccgtg acaggctccc ttttactttt caggcatttc
     2341 tttcagcgat tacagcacca actcgctgcc gttcgacaac tggatcagca ggctgccgtt
     2401 gagcggcgac acgctgccaa aggcggtgcc gttcagcagg atgcggccgg cgttcacgcc
     2461 atcttccacc gacaggtcca gatccatgat gacgccgtcc tggttggtga tgcgaatgcc
     2521 gttgctgctg aaatagtttt caatgtcata ttgtttgcca gcccagcgga tgcggatggt
     2581 gtcgatcagg ccggcagcca ggttggcggg gctggaggtc atggtcacca ccgcacggtc
     2641 cgagtaatcc ggcagatcgg tttcgaaccg cacctgcaca ttgctgtcca caccgccgct
     2701 gatgctcagg gaaccggcga agctgtcgcc gtccaccgag ctaaattcgc cggctgcggt
     2761 cagctggatg ccgatctggt ccaggcccga gtaggcagtt tcgccggaat catcgctttc
     2821 acgcacgttg ctcagatcca gttgcagatt cacctgaccg gcgaagctca tactgtcagg
     2881 gtgctcatcg gttgcgaact gctggtagtt cacctggata gtgccgttgg cgttttccgg
     2941 cagcttgaga ttcttgatgt ccagtccacc ggtgaacagc atttctacat ttccgctgtt
     3001 gatttccagc aggccatgcg cactttccac cgtgccgctg acagcaaatc cgtagcgggt
     3061 actgctgcgg ctggcgcggg tgaaggtcag gtccacttcg gtgtcctgca cgtcgccgta
     3121 aatgcgcact acgccgtcat acagggcgaa gtggccatac acaccggccc ccagaggcag
     3181 ggtcaggtcg ttcaggatgt cgcacagcga gcggttgaaa atcaccaggt tcaaagtgcc
     3241 ttcgcagata tcctccaggg atttgccggc aatcagcaga cggcaggtca tgcgagtcag
     3301 gtagttgccc aggctgtcgc aggcggcacc cagtgccaga tccggcagtg ccacaatcgg
     3361 accgtactgg ccggcaatgg ccatcgcctg cagcatgttg ctcaggtccg cgccggtgga
     3421 aacacccatt gcactaacca cctgggcaaa cgatgcctgc tgtggattca ggctcaggta
     3481 gccctcccac agagccaggt ctgccacgaa ggcatcgatc ttggcggcgg tggcgccgcc
     3541 tgctgtaggt gcgggctggg agtccgtaag gctgccgttt tcagctacct gcaatgtgga
     3601 tttgagctgt tgcagttcag tgacgaagct gcccaggttg aggtgttgtg ccgtagccag
     3661 tgcctgttcc accagatcca gataggaggt gctgccagcg ccgccgtcct gctgtaccag
     3721 ctgaccctgg ttgatgatga actcttcggt aaaggctgcc agtgtttcgc caaaggccac
     3781 atcattggtc agccccatca aagcgccgga cagcagggca tagcgcagtt cgttcgcggt
     3841 ggcgccgctg accgctgttg gattggtcag gtcgatcagg cgagtgccgg tcaggctgcc
     3901 atccaggcca aacaggttct gcacctgtga cagggcggcc gcaatgctca cttcattggc
     3961 accctggggg aactgggcgg ccagctgggc ggccaggtga gtcagcgtgg aaaccgaggt
     4021 ggaaacagac tgctggatgg aaggcagtgc gctgctcagc atgaaggtgc cggacacagc
     4081 gaaacgctcg ccgaaatcga tgctgccatt attgttgctg tcgtaattgc tgctgccgct
     4141 gaaatcaccg cagccagtgg cgctgtcgca ggtcatcagg gtaggttcgc cgctggtggc
     4201 ggcagtcact tcgatataaa ccgcgccctg atagcccgtc acattcaggc tgaaatgacc
     4261 agtctgatcg gtgcgcgtag agccgagcag gcgcgctgtg gtgccgtctt ccagtgcgta
     4321 tacggaaacc agtccgttgc tgatcacgcc cttggtgacc gtgccgctga cagtagaggc
     4381 ctgaacaacg ggcgtcgggg tcgcggaaga cgatccacca ttacttccac cgccaccgcc
     4441 acacgcggtc agcagcgcga ttgcagagga aaccacgaga cggggcagaa cgttgcggga
     4501 aacgggactg ggaaaatgca tgccggagct ctctcactgg gacaggcgaa tacccggcag
     4561 atcggctgcg cgatgggctg caaaggtaat tcaccggtcg gtatccttga ctcatcgtac
     4621 tagtacgatc gtgacactct aaaagtagcc aacaaaaaac ggcctgccgg gtgattttgc
     4681 ggcggcaatc acaaatcggg gtgaatttat agagttgttt aaaaaatgcg caataatcgc
     4741 tggaaacggt taaaaaacag gcggcgggaa actgacgaag ccaggaatgt cctggcttcg
     4801 tataggcggg agtgtcaatt tagccggcgt cggcgccgta tggccagaac cagcatcggc
     4861 aacagcccca gccagcccaa tgcaccgccg gaaaaactgt ggctgtcact gcccgcgcca
     4921 ttggcggaac ggctggaatc tgtggcctgc ttgaccggat catcaaacgt gccagcctcg
     4981 cttactaccg gcagttccag cgatgccggg tactcggggc tgatcaccag ggtgttttgt
     5041 tccggggttg tgtcatagcg ccagtaatcc ggatcgttgc catcgatcac cagcagcacc
     5101 tggctgccgg caggcagatc taccgctgtc cagtagaaat ccagggtgat ctgttccggc
     5161 tgatccggat gcaggccgca gccctcggag ttatggcagg agaagggggc gtggcccacc
     5221 cagttcgctt tgccggtttc cggattcaga tagaacaggt aggcaaaata ctgcgtcttg
     5281 tcctccatat gggcccacag cttgaccttg gcctcaccgc gcagacggac cgggctggtc
     5341 aatgtctggg agttgaacag cagcccatga cggtcttcca cgctggccag gtccaggatg
     5401 cgtccggtgt gttccagcgc gccggtgacc cagccggtgt aggtgatgaa gtccgtggta
     5461 tcgaagccca gcacttgctc gctgtcagtg gtggagtccg tatccaggct gccgcgctgg
     5521 gttcccgcct gggggttcag ctggtaaacc agtgtttgat catgcaccgg cagatcggga
     5581 tagctttccc atttgcgcgt gttcagcacg acggtattga cccgcttgcg ttcgtcgatg
     5641 ccgttgtcct cgcccttcag ataatggtcg agccactgtg cggcatgcac ccagatccgg
     5701 tgatcctcgt cacccagcag accgccggcc tcgccagagg catgggtgcc gcggttcagt
     5761 tccagacgcc agggcccctg gtactgggac agcattcgca cggcagaatc cggttggaac
     5821 agataatcgt gcatgttgtt ggacatgaac agtgccggcg gattggcccg tgcctgggcc
     5881 tgttccatat aggaaatcgg ggagcgaatc agaccccagg ccttcgtttc cgcaatgtcc
     5941 tgatgcacct tcatgttgtt gtagtagtgc tcgatgacag gatcagggcg gtttttcagc
     6001 acatacggga tcggtttgct ggtgcctacg agaatttcca gccaggtgta attgggcgtg
     6061 ttgccggccc acagcgattc aatcagatcg ccccagccgc tcatggctgt tacggccttg
     6121 acgcgctcgt cctgcagggc tgtcagcaat gagatgccgg caccatagga cacacccccc
     6181 ataccgattc gttgcggatc aacgggcagg tgattgttgg ggtccagcag atagtcgatg
     6241 gccgagcgcg catcgctcac atccgcttca cctgcggtat tgatcagacc gggggatgaa
     6301 tgaaagccgc gggcggtgta gctgagaaca acatagccct tgtccgccag caattgtgcc
     6361 tgatccagat actcgtgttc gtacaacgcc cagctgctga tatagatgat ggcgggaaat
     6421 gtggcgccgg catcggcgct ggagggaacg aaaatattgg caggaagctg gatgatctcg
     6481 gagccgggat caaacggatc gagcagggca ggaatcgtga tgtgatcgtt gaaaatccat
     6541 ttgccgtccg ggctgaaccg ttcgactgcc tgagctttac cgaaaaacag acacgcgaat
     6601 acaaacgcta acgcaagacg cagctgcata ggatgttacc cctgtagaga
//
LOCUS       DASUEG010000453         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_994512, whole genome shotgun sequence.
ACCESSION   DASUEG010000453 DASUEG010000000
VERSION     DASUEG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994512"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFX11_08475"
     CDS             <1..652
                     /locus_tag="VFX11_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEX4938694.1"
                     /translation="TYPLAKRTRTLQRLVPVKYWFRVHMMLGILGPVAILFHCNFQMG
                     SLNSSVALTCMLLVVASGVIGRYFYSKVHLGLYGQKASGKALLAAARQQQLQLRQLFA
                     GYPDMAARVDQFYSQLLPQNPENMSVSGAVLAAPRRWYYRLRFRQAIQRHQQADLQQL
                     WRDSHKTLNQYLDTLRKLAQLQFFERLLSWWHILHMPFFIMMLITAVVHVWAVHRY"
     gene            656..2206
                     /locus_tag="VFX11_08480"
     CDS             656..2206
                     /locus_tag="VFX11_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX4938695.1"
                     /translation="MKIRAWLFLVLLMLLPLRANAIDLNLERMLSPGDLSQAHSKYEK
                     DCKSCHSDFKQENQNALCVDCHKDVAQDISAKTGFHGRKPGIANSECRLCHSEHQGLK
                     ADIVQLNPHGFPHQFTDFPLQGLHQPVACNQCHAEGKKFREAPQQCVDCHRKDDVHKG
                     SQGQQCANCHSAEGWRKTVFDHSKTLFPLKGAHSTVECSQCHRGSDYKQAETTCVSCH
                     KIDDVHLNTFGTQCDSCHSTEKWKSVRFNHDRDTDFHLAGAHSTLQCSSCHKPGTLAK
                     ELPQTCVGCHRQNDVHFGKNGEQCDQCHSVNSWGKSRFDHSKTGFPLRGGHANAQCSQ
                     CHQGNVNTPIKDTTCVACHGADDVHNQSLGTQCQQCHSESGWHDNVQFDHELTHFPLL
                     GMHATSACESCHENTRFAGTASACVDCHRTDDTHKGKLGDDCGSCHHPAAWKSWRFDH
                     DKQTPFPLTGAHQGLACDGCHGQTLSQLKGQQCGQCHQADDVHRGAYGRQCDTCHDTR
                     DFSTLEMR"
     gene            2249..3415
                     /locus_tag="VFX11_08485"
     CDS             2249..3415
                     /locus_tag="VFX11_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938696.1"
                     /translation="MRLLVNVFVCGGVLLYSTLFGNAVLAAGFDHDATRFELRGFHEY
                     VECEVCHVNGRFKGTPTDCFACHGGSSQIPGGGKTLNHMFTTNACADCHRPDAWDSIT
                     RFDHSQTQGTCVQCHNGTVQPGKPANHVVSSDQCDSCHNTINWTMARFDHAGITAACS
                     SCHNNSTATGKPGNHVMTNAECDSCHTTRAWTPAGFDHSGITQPCSSCHNGTTATGKH
                     QNHLQTMAECDTCHMTQAWTPANFDHGSVIGNCSSCHNGTTATGKNNGHFVTNRECDY
                     CHVTTRWSPVTYMHQGAGYPGNHARQLDCNDCHQGNVEMNVWTFPAYQPDCAGCHANN
                     FVMDSHKKVDSPTIRYTVSELRDCTGACHLYNDSTFSTVREFRTGEHRVGDGEF"
     gene            3456..>4921
                     /locus_tag="VFX11_08490"
     CDS             3456..>4921
                     /locus_tag="VFX11_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4938697.1"
                     /translation="MVLMLLPVAAHAAPSWVEGINVLYEKPDNAGFQVLLANAFQMAA
                     VESNAGKRCGVRLQPGFSLQIQQERFSAEKLLWDATATLPLESVTSEWVANQLWVQLY
                     FQNTVSCTFQIGANRKTLLVTIRPEDSAATQAINQQLQQAKAALARGDTASAVTIYRA
                     ILEQPPHPLQQDALEYLGVALERQQDYDRAAKIYQAYLNEYPDAAGVPRVRQRLEGLA
                     LMQEAAPPPELRKAKPQAEKETTRWFGVLSNSYQHYSSDQGVGEWENLQSAWITDVNL
                     NGRHRGEEMDVKVLVSAGYWNDFNDETPEPERLSTAYADVYHKSTGQQVRAGRQTTNG
                     EGMLGRYDGVRYSKSLGPQYAVNGIWGHPVISSRDIEINSDTQLYGLSLDITPPQSRW
                     KHNLFVTEQTVNGMLDRQAVGGEVNFIDKNQSWLSYLDYDVNFNELNTVMLNANWFGA
                     DESHYYVALDYRRSPVLTLSNALIGQTLTELDQLAALG"
BASE COUNT         1104 a         1466 c         1428 g          923 t
ORIGIN      
        1 gacctatccg ctggccaagc gcacccggac attgcagcgt ctggtgccgg tcaaatactg
       61 gttccgcgtg catatgatgc tgggcattct cggcccggtg gcgatcctgt ttcactgcaa
      121 tttccagatg ggatcgctga acagctcggt ggcattgacg tgcatgttgc tggtggtcgc
      181 cagcggcgtg atcggccgct atttttacag caaggtgcac ctcgggcttt atggacagaa
      241 ggccagtggc aaggcgctgc tggcggcggc gcggcagcaa cagctgcaac tgcgtcagtt
      301 gtttgcaggc tacccggaca tggcggcacg ggtggatcag ttctatagcc agttgttgcc
      361 gcagaatccg gaaaacatgt ccgtcagtgg cgccgtgctg gcggcacccc ggcgctggta
      421 ctaccggctg cgcttccggc aagccatcca gcgccatcag caggcggatc tgcaacaact
      481 ctggcgtgac tcgcataaaa ccctgaacca gtatctggac accttgcgca aactggcgca
      541 gctgcagttc ttcgagcgcc tgctgtcctg gtggcacatt cttcatatgc ccttcttcat
      601 catgatgctg attaccgccg tggtgcatgt atgggcggtg caccggtact gagaaatgaa
      661 gatacgcgcc tggctattcc tcgttttatt gatgctgctg ccgttgcggg cgaacgccat
      721 cgacctgaat ctggagcgca tgctgagccc gggcgatctg agccaggcgc acagcaaata
      781 cgaaaaagac tgcaagagct gccacagcga tttcaagcag gaaaaccaga acgccctgtg
      841 cgtggattgc cacaaggatg tcgcgcagga catcagcgcc aaaaccggat ttcatggcag
      901 gaagccgggc attgccaaca gcgaatgccg cctgtgccat tccgaacacc agggcctgaa
      961 ggccgacatc gtgcaattga atccgcacgg gttcccgcat cagttcacgg attttccgtt
     1021 gcaggggctg catcagccgg tggcctgcaa ccagtgtcat gcggaaggca agaaattccg
     1081 cgaagcgccg caacaatgtg tcgactgtca tcgcaaggat gacgtgcaca agggcagtca
     1141 gggccagcag tgcgccaact gccactccgc cgaaggctgg cgcaaaaccg tgtttgacca
     1201 cagcaagacg ctgttcccgc tcaagggcgc ccacagcact gtggaatgca gccagtgcca
     1261 ccgcggcagt gattacaagc aggccgaaac cacctgtgtc agttgccaca agattgatga
     1321 cgtgcacctg aataccttcg gcacgcaatg cgattcctgt cacagcacag aaaaatggaa
     1381 atccgttcgc ttcaatcacg accgcgatac ggatttccac ctggccggtg cacatagcac
     1441 actgcaatgt tcctcttgcc acaagcccgg caccctggcc aaagaactgc cacagacctg
     1501 cgtcggctgc catcgccaga acgatgtgca tttcggcaag aatggcgagc agtgtgatca
     1561 gtgccattca gtaaacagct ggggcaagag ccggtttgat cacagcaaaa ccggtttccc
     1621 gttgcgcggc ggccacgcca atgcacagtg cagccagtgc caccagggca atgtgaacac
     1681 gccgatcaag gacaccacct gcgttgcctg tcatggcgcg gatgatgtgc acaaccaatc
     1741 gctgggaacg cagtgccagc aatgtcacag cgaatccggc tggcacgaca acgtgcagtt
     1801 cgaccatgag ctgacgcatt tcccgttgct gggcatgcac gccacatcgg cctgcgagag
     1861 ctgtcacgag aacacccggt ttgcaggcac tgcatcggcc tgtgtggatt gccaccgcac
     1921 cgatgacacg cacaagggca agctcggtga tgactgcggc agttgccatc atccagccgc
     1981 ctggaaaagc tggcgcttcg atcacgacaa acagacgcca tttccgctga cgggtgcgca
     2041 tcagggactg gcctgcgacg gttgccacgg ccagacgctg tcacagctca aggggcagca
     2101 gtgtggccag tgtcatcagg cggatgatgt gcaccgaggc gcgtatggac ggcagtgcga
     2161 tacctgtcat gacacccgcg atttctccac gcttgagatg aggtgaccgg ccatggcaaa
     2221 ccgaattgca acgtggactc catctgtaat gcgtttgctg gtgaacgttt ttgtatgcgg
     2281 cggtgttctg ctatacagca cgctgttcgg caatgccgta ctggcggccg gtttcgatca
     2341 tgatgccacc cggtttgaac tgcgcggctt tcatgaatat gtggagtgcg aagtctgtca
     2401 tgtgaacggt cgcttcaagg gtacgcccac cgactgcttc gcctgtcacg gtggcagttc
     2461 ccagatcccc ggcggcggca aaaccctgaa tcatatgttc accaccaatg cctgcgccga
     2521 ctgccaccgc ccggatgcct gggattccat cacccgattc gatcacagcc agacccaggg
     2581 cacttgcgtg cagtgccaca acggcaccgt gcaaccgggc aagccggcca atcatgtcgt
     2641 cagcagcgat cagtgcgaca gctgccacaa cactatcaac tggacgatgg cacggttcga
     2701 tcacgctggc attaccgcgg cctgttccag ttgccacaac aattcgacgg caaccggcaa
     2761 gcccggcaat catgtcatga ccaatgcgga gtgcgacagt tgccacacta cccgcgcctg
     2821 gacaccggcc ggctttgatc acagcggcat tacccagccc tgttcgtcct gccacaacgg
     2881 aacgaccgcc acgggcaaac accagaatca cctgcagacc atggccgaat gcgatacctg
     2941 ccacatgacg caagcctgga cgccagcgaa ttttgatcac ggctctgtca tcggcaattg
     3001 ctcatcctgc cataacggca ccaccgccac gggcaagaac aacggccatt tcgtgacgaa
     3061 tcgcgaatgt gattattgtc atgtcaccac gcgctggtcg cctgtcacct acatgcatca
     3121 aggcgctggg tatcctggga atcatgcgcg gcagctggat tgcaacgact gtcaccaggg
     3181 caatgtggaa atgaatgtct ggacgttccc ggcataccaa ccggattgcg ccggctgcca
     3241 tgccaacaac tttgtaatgg attcacacaa gaaagtggat tcacccacca ttcgatacac
     3301 ggtgagcgaa ctgcgcgatt gcacgggcgc ctgccacctg tataacgaca gcacattcag
     3361 caccgtgcgg gaattccgta ccggcgaaca cagggttggc gatggcgagt tctgatactg
     3421 atcgccggcg gtcgtgcagc tggttggcgt ggataatggt gctgatgctt ctgccggtgg
     3481 cggcgcacgc tgctccgtcg tgggtggaag gcatcaacgt gctgtatgaa aaaccggaca
     3541 acgccggttt tcaggttctg ctggcgaatg cgttccagat ggccgcggtg gaaagcaacg
     3601 ccggcaagcg ctgcggcgtg cgcctgcagc cgggattttc cctgcaaatc cagcaggagc
     3661 gtttctcggc ggaaaaactg ctgtgggacg ccaccgcgac attgccgctg gaaagcgtga
     3721 catcggaatg ggtggccaac cagctgtggg tgcagctgta ttttcagaac acggtgagct
     3781 gcacgtttca gatcggcgcc aatcgcaaaa cgctattggt caccatccgc ccggaagatt
     3841 ccgccgccac ccaggccatc aaccagcaac tgcaacaggc aaaagcggca ctggcccggg
     3901 gtgacaccgc aagcgcggtc acgatttacc gcgcgatcct ggaacagccg ccacatcccc
     3961 tgcagcagga tgcactggaa tatctgggtg tcgcgctgga gcggcaacag gattacgacc
     4021 gggcggcaaa aatctatcag gcgtacctga acgaatatcc cgacgcagcc ggcgtgccac
     4081 gggtgcgcca gcggctggaa ggcctggcat taatgcagga agcagcgccg ccaccggaat
     4141 tacgcaaagc caaaccgcag gcagagaagg aaaccacccg ctggtttggc gtgctcagca
     4201 attcctacca gcattacagt tccgatcagg gagtggggga gtgggaaaac ctacagtcgg
     4261 cctggatcac tgacgtgaat ctgaatggcc gtcatcgcgg tgaagaaatg gatgtgaagg
     4321 tgctggtgtc ggccggctac tggaacgatt tcaatgatga aacgccggag cccgagcgcc
     4381 tcagcacggc ctacgccgac gtctatcaca agtccaccgg acagcaggtg cgcgccggcc
     4441 gccagaccac caacggcgaa ggcatgctgg ggcgttacga tggcgtgcgc tacagcaaat
     4501 cactcgggcc gcaatacgcg gtgaacggca tctggggcca tcctgtcatt tccagtcgcg
     4561 atatcgaaat caacagcgac acccagctct acggcctgag cctggatatc acgccaccgc
     4621 agtcacgctg gaaacacaat ctgttcgtga cggagcagac ggtgaacggc atgctggatc
     4681 gccaggcggt gggcggcgaa gtgaatttta tcgacaagaa ccagagctgg ctttcgtatc
     4741 tggattacga cgtcaacttc aatgaactga ataccgtcat gctgaacgcc aactggtttg
     4801 gcgcggacga atcccattat tacgttgcgc tggattatcg acgctcaccg gtgctcacgc
     4861 tgtccaacgc gctgatcggg cagactctca cagaactgga tcagctcgct gcactcggct
     4921 t
//
LOCUS       DASUEG010000454         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1763425, whole genome shotgun sequence.
ACCESSION   DASUEG010000454 DASUEG010000000
VERSION     DASUEG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763425"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            687..1484
                     /locus_tag="VFX11_08495"
     CDS             687..1484
                     /locus_tag="VFX11_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit A"
                     /protein_id="HEX4938698.1"
                     /translation="MNTVSGAYGKGGAPDTTSRPTKSAVRNVQEQMAAWKNIDYTMIP
                     IFSILIGGVTVFLIALTVGDWDYWQDWRDRRWWPLVTPFSLIIPAAIFNYFFWKRFRL
                     PIGATAMILGYMFGVWVSRYMNFHIFANFPMNFVSPSTFIGMGLLLDCTLALSRSFFL
                     TGLVGAFLFAIVLYPLNWVTMAPFHVPVEYNGYLVTVADLMGYQYIRTAMPEYVRIIE
                     ESTLRTFGEAVTPLTAFFAGFVTLLNYYLWVWIGSILTQNRWLTRLI"
     gene            1649..>2321
                     /locus_tag="VFX11_08500"
     CDS             1649..>2321
                     /locus_tag="VFX11_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HEX4938699.1"
                     /translation="MLLTLAGPLYSPPADAHGERAQQASLRMRTLHWIDTEIQPRKVA
                     VNETVRVTGRFMPSQFWPEHMRSIEETAFLNIGVPGPAFVRIDSRVNGVPMIRSTRFH
                     LAKTYEYEVVLKARAPGRYHIHPVISVEGAGPIIGPAFWVEVTGDQKDFKHTITTMTG
                     EVINPETYAMTSILWWNALWFVIGIAWLAWWFRKLPVIMPRYIKVEEMGDDANKLITI
                     PDVIVS"
BASE COUNT          462 a          633 c          686 g          540 t
ORIGIN      
        1 acctcggtgg tttcaaatcc ttggccgggg cgccgtccct tcacgcttcg tgattcgacc
       61 tgtgctgagc aagcgcaatt gcgaggagca accgctgcga tgcttgccaa gcaggacagc
      121 aaggcggccg gatccaagcc aaggtgatcc atgctgaact ggatcgggct tgaggcgggt
      181 gtgggcgctt ctggctgcga ttggccattg gtcgctaaga gattgatttt atagcctgca
      241 ggcgatgggc aggaactaaa taggtcggtt gaaaacattt gacctggggc aatcgtcctt
      301 cctgcacggc agcataaact caaaactgtt tgatttggat ggggctattg gcgtgactta
      361 tggccgacgc cggccgcttt ggcggttttt ttcttgaacg gctgttcagg gattgtgcgg
      421 gcctgccgat tcaattacat ttgtgcgcct gaatctcgtg tgatggaatc cagaggcatg
      481 gatgccatac gacgcgaaga taattaacaa atacaataaa ttttccgcat atggtcgcta
      541 tttgctcatg ctgattcagg cgccggcttt taccaggcag gcagggcgga gaagctaccg
      601 ggacacatat tcgacggaag gactgaccga gctgcgcagg atggttcggt ctccggagtt
      661 ttttcagcaa cagggagttc aaggtgatga atacggttag tggtgcatat ggcaagggcg
      721 gagccccgga cacaacatcc cgtccgacca agtcggcggt gcgcaatgtc caggagcaga
      781 tggcggcgtg gaagaacatc gactacacca tgattccgat tttctcgatc ctgatcggcg
      841 gggtgacggt gttcctgatc gccctgaccg tgggtgactg ggattattgg caggattggc
      901 gcgatcgtcg ctggtggcct ttggtgacgc cgttctcact gatcattcct gcggcaatct
      961 tcaattattt cttctggaaa cgcttccggc tacccatcgg ggcgaccgcg atgatcctgg
     1021 gctacatgtt cggcgtgtgg gtcagccgct acatgaactt ccatatcttc gccaacttcc
     1081 cgatgaattt cgtgtccccc tcgaccttca tcggcatggg cctcctgctg gattgcaccc
     1141 tggcactgag ccggagtttc ttcctcaccg gtctggtggg tgcgttcctg ttcgcaatcg
     1201 tgctgtaccc cctgaactgg gtgaccatgg cgcctttcca tgtcccggtg gagtacaacg
     1261 gctatcttgt gacggtggcg gacctgatgg gctaccagta cattcgtacc gccatgccag
     1321 aatatgtccg catcattgag gaatcgacgc tgcgcacctt cggtgaggcg gtgacgccat
     1381 tgacggcctt ctttgcgggc tttgtcacct tgctgaacta ttacctgtgg gtgtggatcg
     1441 ggtcgatcct gacgcagaat cgctggctga cgcgtttgat ctgaactaga ttctgcgcga
     1501 gtttggattc agcccgggga accccggggt tcatcgaaca aggacaccgg ttcgcatgcc
     1561 gcgggccgta cattctggga ttgccgagaa catcatgaac acaatgaaac acatcaggta
     1621 ccgaaattgg gggactttgg cccttgccat gctgctgacc ttggctgggc cgctctacag
     1681 cccacccgca gacgctcacg gtgaacgcgc gcagcaggcc agcctgcgca tgcgcaccct
     1741 gcattggatc gataccgaga ttcagccgcg caaggtcgca gtgaacgaaa ctgtccgcgt
     1801 cacggggcgc ttcatgccgt ctcaattctg gccggagcac atgcgatcga ttgaagagac
     1861 cgcatttctc aatatcggcg tgcccggccc cgccttcgtg cgtattgatt cacgggtcaa
     1921 cggcgtgccc atgatccgtt ccacccgctt ccatctggcc aagacctatg aatatgaagt
     1981 ggtactcaag gcgcgggcgc ccggccgcta ccatattcat cccgtcatca gcgtggaagg
     2041 cgcgggtccc atcatcgggc cggccttttg ggtggaagtg acgggcgacc agaaggactt
     2101 caagcacacc atcaccacca tgaccggtga ggtaatcaac cctgaaacct atgccatgac
     2161 cagcatcctg tggtggaacg cgctgtggtt cgtgatcggg attgcctggc ttgcctggtg
     2221 gttccgtaag ctgcccgtga tcatgccgcg ctacatcaaa gtggaagaaa tgggggatga
     2281 tgccaacaag ctgatcacca ttccggacgt gattgtttcg g
//
LOCUS       DASUEG010000455         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_995405, whole genome shotgun sequence.
ACCESSION   DASUEG010000455 DASUEG010000000
VERSION     DASUEG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995405"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFX11_08505"
     CDS             complement(<1..247)
                     /locus_tag="VFX11_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4938700.1"
                     /translation="MKVLDLIVAGGPVMYPIILCSVVALAIIAERLWALRVNRISPRN
                     LMSRVWGWVKNSQLDAKKVKELRDSSPLGRVLAIGLIN"
     gene            complement(305..2587)
                     /locus_tag="VFX11_08510"
     CDS             complement(305..2587)
                     /locus_tag="VFX11_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX4938701.1"
                     /translation="MAIFILGVPFALVLSLLLLDPRPLTAFWWLAGAMLAAWAGVLWR
                     RGPKRLHQAFGLGLALLLGWQLAGLGSVRFAPWALQTDRSYHITGTVSGLPDSRFYRH
                     RLLLRPDCVSSGENLPCDRWSGESRLWPVQLEISIPRQHLKQTPRPGQRWEFNAHFVP
                     AESSRNPGGFDVARWYRSQHVVARFQVGPGDSSLLVEDSAAALDRARFALREALVRQA
                     ENTPAVDRTGLAIPLALVTGDRSLMQQEQWALFNATGTTHLVAISGSHIVLVAGVVMW
                     LLQFLLRRWIWLTHRVPASHVAAAGAFATACLYGAIAGLGLPVQRALVMLAVFVVLQL
                     AGRVQQLWFALALAFCLVLVWDPMAVYALGFWLSFMAVYWILWMSGGAVFRPGWVRGW
                     LRVQAGIFVGLAPVLLWQLQNVSLVSGITNAFAMPLVGMVLTPLALVWALAWGLIGDA
                     ANALLIPAGWLTDVWLVALGWFADLRYAVLQVPPRPVSAMLLGLAGSLWICSAGLPAR
                     WLAPLLFLPLLQPLVQSAQREGELWLRDSGGPLQLAIVLPDRALFVGASPWPQLLASW
                     QQGFLTYWGIGVPALGTPLEGSAAFWSAESWTFTQLPLQTNGLGEPEVQQIRLGDLCQ
                     QDALPDGPVQTQVLYRQGARCVVRLELGGESWLLWPTLSQSAQQSLLQTAKSKLAVPR
                     ILFNPARQDRLLPALLSFWQQQGAELILTDAPAPELLRQLEHGALKFRVLAGSGPLRF
                     PLHRQPVHDGRNAIRSAAAF"
     gene            2720..3244
                     /locus_tag="VFX11_08515"
     CDS             2720..3244
                     /locus_tag="VFX11_08515"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX4938702.1"
                     /translation="MKKFFQKYSPDAQSVRSNRLVAKLGPRLTDPNLWHLNRRSVSAG
                     VFAGIFSAFMPPGLQLILAIPLAMLIRGNLAVAFASTWVTNPFTYLPVYFACYRLGLW
                     LLGQNANITDAQFNLDHLAANLWDVGQPLLVGCSVSAVFFGGLCYGLVRLLWRLSVLS
                     HIRQRTLRRKQKVS"
     gene            complement(3299..>3922)
                     /locus_tag="VFX11_08520"
     CDS             complement(3299..>3922)
                     /locus_tag="VFX11_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4938703.1"
                     /translation="SVNFRLERGELVAIIGNSGSGKTTLLHLLSGLDVPSSGSVLING
                     RDFSSLNDKERGIVRNRNIGFVYQFHHLLPEFSALENVSMPLLLRKEDSIPAVKKRAA
                     EMLERVGLGHRVSHKPSELSGGERQRVAIARALVTKPGCVMADEPTGNLDVHTAREIQ
                     GLMHELNRDLQISFLIVTHDLGLARQVGRVVRMEDGHLLDATAGDLA"
BASE COUNT          843 a         1344 c         1113 g          622 t
ORIGIN      
        1 cgttgatcag gccaatggcc agcacccggc ccagcggcga ggaatcccgc agttccttca
       61 cttttttcgc atccagctgg ctgttcttga cccagcccca tacgcggctc atgagattgc
      121 gcgggctgat ccggttgacg cgcagggccc agagacgttc ggcaatgatc gccaacgcca
      181 ccaccgaaca gagaatgatg gggtacatca ccgggccacc ggccactatt aaatcgagca
      241 ctttcacgcg ccataatctc ctgttttttt aatgtttttt actgtagcac actgtagcag
      301 ctttttagaa cgccgctgca gacctgatcg catttcggcc atcgtggaca ggctgtcggt
      361 gcaacggaaa acgcaggggg ccggacccgg ccagcacacg aaacttcagg gcgccgtgct
      421 ccagctggcg caacaattcc ggcgcaggcg catcagtgag aatcagttcc gcgccctgct
      481 gctgccagaa ggacaacagg gccggcagca accggtcctg gcgggccgga ttgaacagaa
      541 tgcgcgggac cgccagcttg cttttcgctg tctgcagcaa cgattgctgg gctgactggc
      601 tcagagttgg ccacagcagc caggattccc cgccgagctc cagccgcacc acgcagcggg
      661 cgccctggcg gtacagcacc tgtgtctgca ccggcccgtc aggtaatgca tcctgctggc
      721 acaggtcacc caaccggatc tgttgcacct cgggttcacc cagtccgttg gtttgcagcg
      781 gcaactgcgt gaaggtccag ctttccgccg accagaaggc ggcactgcct tccagtggcg
      841 tacccaaggc aggaacacca atgccccagt aagtaagaaa gccctgctgc caggacgcca
      901 gcaactgcgg ccagggactg gcaccaacga acaacgcgcg gtccggcaac acgatggcca
      961 gctgcaacgg accgccactg tcccgcaacc agagctctcc ttccctttgc gcggactgta
     1021 ccagcggctg caacaacggc aggaacaaca acggcgccag ccagcgcgcg ggcaatcctg
     1081 cggagcaaat ccataacgat cccgccagcc ccagcagcat ggcgctgacc ggccggggcg
     1141 gcacctgcaa caccgcataa cgcaggtcgg caaaccagcc cagcgctacc agccagacat
     1201 ctgtcagcca gccggccgga atcaacagcg cattggccgc gtcaccgatc aatccccatg
     1261 ccagcgccca caccagcgcc agcggcgtca gcaccatgcc caccagcggc atggcgaagg
     1321 cgttggtgat gcccgatacc agcgacacat tctgcaattg ccacagcagc accggcgcca
     1381 gcccgacaaa aattcctgcc tgcacccgca gccagccacg cacccagccc ggacgaaata
     1441 cggcaccgcc gctcatccac agaatccagt acaccgccat gaacgacagc cagaaaccca
     1501 gcgcatagac cgccatcggg tcccacacca gcaccaggca aaacgccagt gccagcgcga
     1561 accagagttg ctgcacccgc cccgcaagct gaagcactac gaacaccgcc agcatcacca
     1621 gtgcccgctg caccggtaac cccaggccgg caatggcacc atacagacac gctgtggcaa
     1681 acgccccggc cgccgcgaca tgggacgccg gcacacgatg ggtgagccag atccagcgcc
     1741 gcaacaggaa ctgcagcagc cacatgacca cccccgccac cagcacgata tgggagccgg
     1801 aaatggccac cagatgggtg gtaccggtgg cgttgaacaa tgcccactgc tcctgctgca
     1861 tcagggaacg atcgccagtc accagcgcca ggggaatggc aagccccgtc ctgtccaccg
     1921 ccggcgtgtt ttccgcctgc cgcaccagcg cctcccgcag ggcaaagcgg gcccggtcca
     1981 gcgccgcggc cgaatcctcc accagcaagg acgaatcacc cggacccacc tgaaaacgtg
     2041 ccaccacatg ctgcgaacgg taccagcgcg cgacatcgaa accgccggga ttacgggatg
     2101 actccgctgg aacgaagtgg gcgttgaatt cccagcgctg ccccggcctg ggcgtctgct
     2161 tcaggtgctg gcgcggaata ctgatttcca gctgcactgg ccaaaggcgc gattcgcccg
     2221 accaacgatc acaaggcaga ttctcgccac tggaaacgca atcgggccgc agcagcaagc
     2281 ggtgccggta gaagcgggaa tccggcaagc cgctgacggt gccggtaatg tgataggaac
     2341 gatctgtctg cagggcccag ggcgcaaagc gcacgctgcc cagtcctgcc agttgccaac
     2401 ccagcagcaa ggccaggccc agtccgaagg cttgatgcaa ccgtttcggt ccgcgccgcc
     2461 aaagcacgcc agcccaggcc gccaacatcg cccccgccag ccaccagaat gcggtaaggg
     2521 ggcgaggatc gagcaacagc agactcagaa ccagcgcgaa gggaactccc agaatgaaga
     2581 tggccatccg tggtacctgt cgtcaccaag tgacagtatt acagtaaacg ggatgtgtca
     2641 tgacgcatcc ctcgctggct ggtctaacca ggatcgggcc ggcataatgg ccgctcgaaa
     2701 accatcccag gcggcgctct tgaagaaatt cttccaaaaa tattcacccg acgcgcagtc
     2761 cgtccgcagt aaccgcctgg tggcaaaact cggcccccga cttaccgatc ccaacctgtg
     2821 gcatctcaat cgccgttcgg tgtcggccgg ggtatttgcc ggcatcttct cggccttcat
     2881 gccaccgggc ctgcagctga tactggcaat tcccctggcg atgctgatcc ggggcaacct
     2941 ggcggtggcg ttcgcttcca cctgggtcac caatccattc acctatctgc cggtgtattt
     3001 tgcctgctac cgcctggggc tttggttgct gggccagaac gccaacatta ccgacgcgca
     3061 gttcaatctg gatcacctgg ccgccaacct gtgggacgtg ggtcaacccc tgctggtggg
     3121 atgcagtgtc agcgccgtgt ttttcggggg gctctgttac ggtctggtgc ggctgttgtg
     3181 gcggctgagc gtgctcagcc atatccggca gcggacgctt cgccgtaaac agaaagtgtc
     3241 gtgaaagaaa acaactaccc cgccttaaga gacagccgcc acaacaacta aacgcgcact
     3301 aggccagatc gccggcagtc gcgtccagca aatgcccatc ttccatccgc accaccctgc
     3361 ccacctggcg tgccagaccc agatcgtggg tgacaatcag gaacgagatc tgcaggtcgc
     3421 ggttgagttc gtgcatcagc ccctggattt cccgcgcggt atgcacgtcg agatttccgg
     3481 tgggctcatc cgccatcacg cagcccggct tggtcaccag cgcccgggcg atggccaccc
     3541 gctgccgttc accgccggac agctccgacg gcttgtggga aacgcgatgc cccagcccta
     3601 cccgctccag catctcggcc gcacgctttt tcacggcggg aatggaatct tccttgcgca
     3661 gcagcagcgg catggacacg ttttccagcg cactgaattc cggcaacaga tgatggaact
     3721 ggtagacgaa accgatattg cggttgcgca caatgccgcg ctccttgtca ttcagggagc
     3781 tgaagtcgcg gccattgatc agcacgctgc cggacgaggg cacatccagc ccgctcagca
     3841 ggtgcagcag cgtggtcttg ccggagccgg agttgccgat gatcgccacc agctcacccc
     3901 tttccaggcg gaaattgacg ga
//
LOCUS       DASUEG010000456         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_4113, whole genome shotgun sequence.
ACCESSION   DASUEG010000456 DASUEG010000000
VERSION     DASUEG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4113"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VFX11_08525"
     CDS             complement(<1..1280)
                     /locus_tag="VFX11_08525"
                     /inference="COORDINATES: protein motif:HMM:NF018259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HEX4938704.1"
                     /translation="MTKRRVRKPLQIQVAQPEAVESRYTWRTDWVNRYESSYSLLSKF
                     AHLNGLGLQQLVTLFVRREPRGKVTTARHSGIDLISMGVISASALSTHAQLSGLQVQQ
                     ASLEGSWDKGMYPWTADFRFCPACLAACYHSTLHQLVFLAKCPAHNRRLQTHCPQCHA
                     VMPYRFTAAVAANPFCCPHCELPWAPALFEKAKGYPLLHQRHVERLSKTWKVLSGDRR
                     RFGVLVNCSTHPAFARDLENGHQLALMARRREEFFSFLARLDRAVAQDAAQLHMDDQD
                     LLQLHSGPMASHAGYGPAEKDTKTTRPWFENDSKFWSVLPVYKAICRHFLHHVVAAHR
                     DCIAATVRHLWWHMEGRASEPICPVAYAFVMWRMFWEECGTPHQLMEPPRHAPYRILA
                     WLSDAAPYCPATWPIETQEWLRHRVFAADCLATF"
     gene            complement(1273..>2221)
                     /locus_tag="VFX11_08530"
     CDS             complement(1273..>2221)
                     /locus_tag="VFX11_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4938705.1"
                     /translation="PLTNKDYTIPTPAIDAMVSMAMRCIEQRIPGAILEGINRSGKTT
                     GIGYTIRTLKNEVPGAAVYRFSCTRDLTDNPNDFFSHLLYSFNHPDPFSGKCRQKRVR
                     LTEMLMKDAGKGRRGLVVIFADEAQRLSYNMYAWLADIYNELDAKRVTVITFLVGQPD
                     LANQKVALIKANRMEIVGRFMVQHLRFNGLTSASDVATCLNAYDESCCPEGSGWSYTR
                     FFFPKAYLCGMRAVSSATTLWNVFQQHHEVAGSPGELEIPMQYFAGAVEHIFRTYSGD
                     DNADFGVSERMWAGAVEETGFKALEENLRLRMTMSVQDD"
BASE COUNT          493 a          649 c          608 g          471 t
ORIGIN      
        1 tcaaaggtcg caagacagtc cgctgcaaac acacgatgcc gcagccattc ctgcgtttca
       61 ataggccatg tcgctggaca atatggtgcc gcgtccgaca accacgccag gatgcgatat
      121 ggtgcatgac gtggaggctc catcagctga tgtggagttc cacattcttc ccagaacatg
      181 cgccacatca cgaaggcata cgccaccgga cagatcggct ccgacgctcg cccctccata
      241 tgccaccaca gatgcctcac tgtcgcagca atgcaatcgc gatgtgccgc aacgacatgg
      301 tgcaaaaaat gccggcaaat ggctttgtag acggggagta cagaccagaa cttactgtcg
      361 ttctcgaacc aaggtcgcgt ggtcttggtg tctttctcgg cgggcccata gccagcatgc
      421 gaggccatgg ggcccgaatg caattgcagt aggtcctgat cgtccatgtg cagttgggct
      481 gcatcttgag caacggcccg gtccagtctg gccaagaagc tgaaaaactc ctctcgacgg
      541 cgtgccatca atgccaactg atgaccgttc tccaggtccc ttgcaaaggc cggatgggtt
      601 gagcaattga ccagcacacc aaagcgcctg cggtcgccag acaggacctt ccatgtcttg
      661 ctcaaccgct ccacgtggcg ttgatgaagc agcggatacc ccttagcttt ttcgaaaagt
      721 gctggcgccc agggcagttc gcagtgtggg cagcaaaaag gattggcggc tacggcggcc
      781 gtgaagcgat acggcatgac tgcgtgacac tgcgggcagt gggtttgcaa ccggcgattg
      841 tgtgctgggc acttggcaag aaacaccaat tggtgcagcg tgctgtggta gcacgctgcc
      901 aggcaggcgg ggcaaaaccg gaaatccgca gtccacggat acatgccttt gtcccacgag
      961 ccttccagag aagcctgttg gacttgtagc ccggacaact gagcatgtgt cgacagagcc
     1021 gaggcggata tcacgcccat cgagatcaga tcgataccgc tatgccgtgc agtcgtcacc
     1081 ttccctcttg gctctcgccg aacaaacagc gtaacgagct gctgcagacc caacccattc
     1141 agatgggcaa atttgctgag taagctatag ctagactcat agcggtttac ccagtctgtt
     1201 cgccaggtgt atcgagactc caccgcctcc ggttgtgcca cctggatctg cagcggcttg
     1261 cgaaccctgc gcttagtcat cttgcaccga catggtcatg cgcagacgca gattttcttc
     1321 cagtgccttg aagccggttt cttcgacggc accggcccac attcgctcac tgacgccgaa
     1381 atccgcattg tcgtcaccgg agtaggttcg aaagatgtgc tcaactgcac ctgcaaagta
     1441 ctgcatgggg atttcgagtt cgcctggcga acccgccact tcatgatgct gctgaaagac
     1501 attccacaat gtcgtcgccg aactgaccgc acgcatgccg cacagatagg ccttgggaaa
     1561 gaagaaccgg gtataactcc agcccgatcc ttccgggcag caggattcat cgtaggcatt
     1621 cagacaggtg gcgacgtctg acgcggaagt caacccgttg aagcgcagat gctggaccat
     1681 gaaccggccg acaatttcca ttctattggc tttgatgagc gcaaccttct ggttggcgag
     1741 atcgggctga ccgacgagga atgtgatgac cgtcacccgc ttcgcgtcca gttcattgta
     1801 aatgtctgcc aaccacgcat acatgttgta ggacagccgc tgtgcttcat cggcgaaaat
     1861 caccacaagt ccacgccggc ccttaccggc atccttcatc agcatttcgg tcagtcgcac
     1921 tcttttctgc cggcacttcc cgctgaatgg gtcgggatga ttgaaggagt acagcaagtg
     1981 ggaaaagaag tcattcgggt tgtcggtcag atcccgcgtg caggagaacc gataaacggc
     2041 agcacctggt acctcatttt tcagcgttcg tatcgtgtaa ccgatcccgg tcgtctttcc
     2101 gctgcggttg atcccttcca gtatcgcccc tggaatacgc tgctcgatgc agcgcatcgc
     2161 cattgacacc atggcgtcaa tcgccggcgt gggaatcgta tagtccttgt tcgtcaacgg
     2221 a
//
LOCUS       DASUEG010000457         5100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_665942, whole genome shotgun sequence.
ACCESSION   DASUEG010000457 DASUEG010000000
VERSION     DASUEG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665942"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFX11_08535"
     CDS             complement(<1..536)
                     /locus_tag="VFX11_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX4938706.1"
                     /translation="MSTDALCWINGAIIPAHTATISVFDHGLLYGDGVFEGIRFYHRR
                     SFRLQEHLARLERSARAIALQLPYTLAQMADFIDATVNAAKFDNGYLRVVVTRGAGSL
                     GIDPRNCTQPGFFIIADTLQMVKAEARQQGARLIIASTRRLPMDGLDPRVKSLNYLNH
                     ILARIEANQAGMDEAILL"
     gene            complement(526..1347)
                     /locus_tag="VFX11_08540"
     CDS             complement(526..1347)
                     /locus_tag="VFX11_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX4938707.1"
                     /translation="MAHATCKECFVVSAFTDQPFSGNPAAVCLLDQPRPDTWLQAVAA
                     DFNLSETAFLLPLASGRWQLRWFTPTVEVDLCGHATLASAHVLWREQKIATQVLEFET
                     RSGVLTAQQRNGLQTGEQIALDFPASELQPVTLQPHWQDAVQGKAINAVRGRQDLLLE
                     LQTEQQVRDFVPDFAAIKQLPARGLIITARSQSGDADFVSRFFAPGVGVDEDPVTGSA
                     HCILGPYWGQQLDKTTLRGHQVSRRGGFVGVELHGTRLNLIGSAITTLRGTLHVD"
     gene            1502..2914
                     /locus_tag="VFX11_08545"
     CDS             1502..2914
                     /locus_tag="VFX11_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX4938708.1"
                     /translation="MDHLYTQLAADLTDRIEQGLLRPGDRLPGVRELARQRGLSVATA
                     VAAYRALETGGQIEARPRSGFYVRAQTRAVIVEPEQSNPLPKPTPVSGQDMVLRLAQA
                     ASDPSILQLGAAVPDPAFLPTRAIERALASAAHHHRVRSSAYEFPPGLPALRHQIARR
                     MAAAGARVHPDEIIITNGCQEALTLALQTVTRPGDIVALESPTFYGLLQVVEALGLKA
                     LEIPTHPRTGLSLEALQLALEQWPVKACVAVTNFSNPLGHCMSDGQKQALVELTARYS
                     VPLIEDDIYGDLGHRHQRPGTAKAFDTGGNVLYCSSVSKTLSPGLRIGWIAPGRFREQ
                     VLYRKYVTNLATSTVTQLAVADLLESGRYDRHLRVMQGEFARAVQRMTAAVSRHFPPG
                     TRITQPEGGFVIWVELPAAIDSFELSGRALQAGVSIAPGPIFSATGKYRNFLRLSCAC
                     QWNERTETALVKLGQLVASA"
     gene            3058..>5100
                     /locus_tag="VFX11_08550"
     CDS             3058..>5100
                     /locus_tag="VFX11_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4938709.1"
                     /translation="MRFGSAFESRTVLDLVLLLAALWMQGCGKSSDSDMDGDGVPEPY
                     GMETRPTLAPLNIPLTPASSGDPALVTAFPGLPAFTSPVYLTHSGDGTNRLFVVEQHG
                     VIKVFNNTPDVSSASVFLDITDRVVDGGELGLLGLAFDPDYESNGYFYVYYTAASPLR
                     SVLSRFRVSTGNANQANVASETVLMQVDQPYENHNGGTITFGPDGMLYWGLGDGGSAG
                     DPQNNGQNNLSLLGKMLRINVRGVPTYTVPADNPFVGNASYRPEIWALGLRNPYRFSF
                     DRENGRLWLADVGQYEIEEIDVIEKGKNYGWNWYEGTRQYRDGAPSASLFEFPVFEYD
                     HNDGVSITGGYVYRGSQVLALQGKYVYADFASSHVWVLTVDDNLNVQSNTLLATSEQN
                     VSAFGEDEAGELYVVGYGGTIYAVRAESSSDPLAGFPAQLSDTGLFSDTTSLTPASGL
                     IEYSVQAPLWSDHAEKTRWIALPNSTRISFDANGNWQFPVGTVLVKHFGMEMTAGDPT
                     TTRRLETRVLVRQTGSWVGVTYRWNAQETDATLQASSATETLVVNDSTAPGGVRTQEY
                     FYPGSSECLLCHTPVAGRVLGVRTRQLNHSFAYSGVTDNQLRAWNHIGLFTTNIGRFD
                     SYASHRALDDTSATLEARARSYLEVNCAFCHTAGGTAPSALNFSSTVALAAINAVDV"
BASE COUNT          938 a         1635 c         1598 g          929 t
ORIGIN      
        1 ttgagcagga tcgcttcgtc catgcccgcc tggttggctt cgatacgcgc cagaatgtgg
       61 ttcaggtaat tcaggctttt aacgcgcgga tcaaggccat ccatgggcag gcgacgggtg
      121 gatgcgatga tcagccgcgc gccctgttgc cgcgcttctg ctttcaccat ctgcaacgtg
      181 tcggcgatga tgaagaaacc cggctgcgtg caattgcgcg gatcgatgcc cagggaaccg
      241 gcgccgcgcg tgaccaccac tcgcagataa ccgttgtcaa acttcgctgc attcacggtg
      301 gcatcgatga agtccgccat ctgcgccagc gtgtacggca attgcaatgc aatggcgcgc
      361 gccgagcgct ccagccgcgc cagatgttcc tgcaaacgga aactgcggcg atggtagaaa
      421 cggatgcctt cgaacacccc gtcaccgtac agcagaccgt gatcgaacac ggaaatggtc
      481 gccgtgtggg cgggaatgat ggcgccgttg atccagcaga gtgcgtcagt cgacatgcag
      541 ggttcctcgt aatgtggtga tggccgagcc gatcaggttc aaacgtgtgc cgtgcagttc
      601 cacgccgaca aagccgccgc gtctggaaac ctgatggccg cgcagtgtgg tcttgtccag
      661 ttgctggccc cagtacgggc ccaggatgca gtgggcggag ccggtgaccg gatcttcatc
      721 cacgcccacg cccggcgcga aaaagcgcga tacgaaatcg gcgtcgccgg actggctgcg
      781 ggcggtgatg atcaggcccc gcgccggcaa ctgtttgatg gcggcaaaat ccggcacaaa
      841 gtcgcgcacc tgctgctcgg tctgcaactc cagcagcaga tcctggcggc cgcgtacggc
      901 gttgatcgcc ttgccctgca ccgcatcctg ccagtgcggc tgcaatgtca ccggctgcaa
      961 ttccgacgcg ggaaaatcca gcgcgatctg ctcgcccgtt tgcagcccat ttcgttgctg
     1021 tgccgtcagc acaccgctgc gggtttcgaa ttccagcact tgcgtcgcga ttttttgctc
     1081 ccgccacaac acatgggccg acgccagcgt ggcatggccg cacagatcca cttccaccgt
     1141 gggcgtgaac cagcgcagtt gccagcgtcc gctggccagc ggcagcagaa acgcggtttc
     1201 cgacaggttg aaatccgccg ccaccgcctg cagccaggta tccgggcggg gctggtccag
     1261 caggcacacc gccgccggat tgccgctgaa gggctggtcg gtaaaggcgc tgacgacaaa
     1321 acattccttg caggtggcgt gggccatgac gggattcctc gtgtccgtgc gaaaaggagt
     1381 ttcactttag ggggtggcgg cgctacagta cagattcaca ttgaaaaaat tgaaccggta
     1441 cagattggtt gattcagcct tctgtaccgg ttcgatccga tgctatctgt acaggtgcag
     1501 catggaccat ctttacactc aactggcggc ggacctgacc gaccgtatcg agcagggtct
     1561 gctgcggccc ggcgaccggc tgcccggggt gcgggaactg gcccgccagc gcggcctcag
     1621 cgtggccacg gcggtggcgg cctaccgcgc gctggaaacc ggcgggcaga tcgaagcgcg
     1681 accccgctcc ggtttttatg tgcgggcgca gacaagggcg gtgatcgtcg agccggagca
     1741 gtccaatccg ctgccgaagc ccacgccggt cagcggccag gacatggtgc tgcggttggc
     1801 ccaggcggcc agcgatcctt ccatcctgca gttgggggcg gcggtgcccg atcctgcctt
     1861 cctgcccact cgcgccatcg agcgcgccct ggccagtgct gcccaccatc accgggtccg
     1921 cagcagcgct tatgaatttc cccccggtct gccggccctg cgccaccaga tcgcccggcg
     1981 catggcggcg gccggcgccc gcgtccaccc cgatgaaatc atcattacca acggatgtca
     2041 ggaagcgctc acactcgccc tgcaaaccgt cacccgccct ggagacatcg tggcgctgga
     2101 atcccccacc ttctacggcc tgctgcaggt agtggaggcg ctgggcctga aggccctgga
     2161 aattcccacc catccgcgca cgggactgtc gctggaggcg ctgcaactgg cactggaaca
     2221 gtggcccgtg aaggcctgcg tggcggtgac caacttcagc aatcccctgg gccactgcat
     2281 gagcgacggg cagaaacagg ccctggtgga actgactgcc cgctacagcg tgccgctgat
     2341 cgaggacgac atctacggcg atctgggcca ccgtcatcag cgcccgggca ctgccaaggc
     2401 gttcgatacc ggcggcaatg tgctgtactg ctcctctgtg tcgaaaaccc tgtcgccggg
     2461 actgcgcatc ggctggatcg cgccggggcg tttccgcgag caggtgctgt accgcaagta
     2521 cgtcaccaac ctggccacct ccaccgtgac ccagctggcc gtggcggacc tgctggaaag
     2581 tggccgttac gaccgtcacc tgcgggtcat gcagggggag ttcgcccgcg ccgtgcagcg
     2641 catgacggcg gcggtgagcc ggcattttcc gcccggcacc cgcatcaccc agccggaggg
     2701 cggctttgtc atctgggtgg aactgccggc cgccatcgac agcttcgagc tcagcggccg
     2761 ggcgctgcag gcgggagtca gcatcgcgcc gggtccgatc ttttccgcca ccggcaagta
     2821 ccggaatttc ctgcgcctgt cctgcgcctg ccagtggaat gagcggaccg aaaccgcact
     2881 ggtgaaactg gggcaactgg tggcgtcagc ctaagccgtt ttgacctgaa tgccattttg
     2941 cgaacccgga ctgaccgcgc ggcgactgac gattgcgcgc ggccggaaat cggcgcatct
     3001 ttcgccggtg cccagactga agggccgcat aattgatact gtcaccgggg atgttccatg
     3061 cgttttggtt ctgcttttga gtctcgtact gttttagacc ttgtcctgtt gctggctgcg
     3121 ctgtggatgc agggatgcgg caaatcgtcc gactccgaca tggatggcga tggcgtgccg
     3181 gaaccctacg gcatggaaac ccgtcccacc ctggcgccgc tgaatattcc cctgacgcct
     3241 gccagcagcg gtgatcctgc cctggtgacg gcctttcccg gactgcccgc tttcaccagc
     3301 ccggtgtatc tcacccacag cggcgacggt accaaccgcc tgttcgtggt ggaacagcac
     3361 ggcgtaatca aggtgttcaa caataccccc gacgtgtcca gcgccagcgt atttctcgac
     3421 atcactgacc gtgtagtgga tggcggcgaa ctggggctgc tgggcctggc gttcgatccg
     3481 gattatgaaa gcaacggcta cttctacgtg tactacaccg ctgccagtcc gctgcggtcg
     3541 gtgctgtcgc gcttccgggt gagcaccggc aatgccaatc aggccaacgt ggcgtcggaa
     3601 acggtgctga tgcaggtgga ccagccttac gaaaaccaca acggcggcac catcactttc
     3661 ggcccggacg gcatgctgta ctggggcctg ggcgatggcg gttctgccgg cgatccgcag
     3721 aacaacggcc agaacaacct gtcactgctg ggcaagatgt tgcgcatcaa cgtgcgcggc
     3781 gtgcctacct acacggtgcc cgccgacaac ccgttcgtgg gcaacgccag ctaccggccg
     3841 gaaatctggg cgctgggtct gcgcaatccc taccgctttt ccttcgaccg cgaaaacggc
     3901 cggctctggc tggcggacgt gggccagtat gaaatcgaag aaatcgatgt gatcgaaaaa
     3961 ggcaagaact acggctggaa ctggtacgaa ggcacccgcc agtaccgcga tggcgcgccg
     4021 tccgccagcc tgttcgaatt tccggtattc gagtacgacc acaatgacgg cgtttccatc
     4081 accggcggtt acgtgtatcg cggttcgcag gtgctggcgc tgcagggcaa atatgtgtat
     4141 gcggatttcg ccagctccca tgtctgggtg ctgacggtgg acgacaacct gaatgtacag
     4201 agcaacacgc tgctggccac gtccgagcag aatgtcagcg cctttggcga ggatgaagcg
     4261 ggcgagcttt atgtggtggg ttacggcggc acgatttacg cggtgcgcgc cgaaagcagc
     4321 agcgatccgc tggcgggctt tcctgcgcaa ttgtcggata ccggactgtt cagcgacacc
     4381 accagcctga caccggcgtc cggcctgatc gaatactcgg tgcaggcgcc gctgtggtcc
     4441 gatcatgcgg aaaaaacccg ctggatcgcg ctgcccaaca gcacccgcat cagcttcgat
     4501 gccaacggca actggcaatt tcctgtcggc acggtgctgg tgaaacattt cggcatggaa
     4561 atgacggcgg gtgatcccac cacgacgcgc cggctggaaa cccgcgtgct ggtgcgccag
     4621 accggcagct gggtgggcgt cacctatcgc tggaacgcgc aggaaaccga tgccacgctg
     4681 caagccagtt ccgctaccga aaccctggtg gtgaatgaca gcacggcgcc cggcggcgtg
     4741 cgcacgcagg aatatttcta tcccggcagc agcgaatgcc tgttgtgcca cacgccggtg
     4801 gctggccgtg tcctgggcgt gcgaacgcgt cagctcaacc acagcttcgc ttacagcggc
     4861 gtgaccgaca accagctgcg ggcgtggaat cacatcggcc ttttcactac caacatcggc
     4921 cgcttcgaca gctacgccag ccatcgcgca ctggatgaca ccagcgccac gctggaagcc
     4981 cgcgcccgtt cctatctgga ggtcaactgc gccttttgtc acaccgcagg aggcactgcg
     5041 ccctcggcgc tcaacttcag cagcaccgtg gcgctcgcgg ccataaatgc cgtcgatgtg
//
LOCUS       DASUEG010000458         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_276470, whole genome shotgun sequence.
ACCESSION   DASUEG010000458 DASUEG010000000
VERSION     DASUEG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276470"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFX11_08555"
     CDS             <1..1039
                     /locus_tag="VFX11_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4938710.1"
                     /translation="SSGGTAAAVAAGIVPFGSAGDGGGSIRIPSACCGLFGLKPSRGL
                     VPSGPDYGEIWDGASVEHVITRSVRDSAAMLDVLAGSDAASHVALPFKTDSFLQALEQ
                     PPPKLKIGFSTESPMGGTVAVESRDAVRNVAELLQSLGHVVEQAAPAGIDASRLSRAY
                     ADIYLAHVLADVLQVERAHGRCYAARNFEPATLFIANLGRRFSAGAYLRSRQYWVQLR
                     RLMADFHKRYDLWLTPVLAAEPYRLGELRSSRVEEQAMDVLNHTGLHRLVPMAQYYRI
                     TLQQLRRVPFTQIANLTGQPAMSVPLVWSHKGLPMGVQLVGPVGSDRRLLQLARQLEQ
                     ARPWFGRQPQL"
     gene            complement(1075..2424)
                     /locus_tag="VFX11_08560"
     CDS             complement(1075..2424)
                     /locus_tag="VFX11_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4938711.1"
                     /translation="MADFDPLHDKAYAQRLTQLGEKREIVASEDIYNDQGILLIKKGS
                     PINERMADKIIRFKLIKPIEASVDINNSLSAVDLYRDLMEILDRYPQIKLVHQRIEID
                     SAIKQLCSSYAKFAILKQKLTVLKEQMNQLYRQSLGIAWLSVVIAHNMKLTAQQIETC
                     FLAALAHDIGMMHIDPLVVNKQDTLTPAEWRQIQAHTIIAQKVLERISGMDPRVALAV
                     SEHHERCDGSGYPAGKFAHHLCIEGQVIALSDSVIAVFTKRLLPNGRNLRDLMPLLQV
                     NSESHFYDTYKALILILRNADLDESPFTTNESLDAQIDKLTRDNDRLSLLLNSLEQVV
                     GLFKEGVNHKLLTSAKTVLIQVMRIVRGSGILDAGYLRWLQQVRKDRLSFAYREIDDV
                     SLMLDEIEWHLVRVKRMLDAFIEQGPVSENRLKDLIRSELKKTELQDSTNRDEFAIT"
     gene            complement(2647..3168)
                     /locus_tag="VFX11_08565"
     CDS             complement(2647..3168)
                     /locus_tag="VFX11_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX4938712.1"
                     /translation="MVLLLLTTTLTRADTDLELVKEYQVKALFLYNFANFVEWPDNAF
                     ENPGSPLKMCLYGAVPFGEFLDVVNGAQIRSRFLQVIRTTDYAKIKTGCHILFVGIDH
                     VGELESFFRDFNHLFVLSIGNTEDFARKGGVVNILRTRDQQQFEINLSKAMESGLLIN
                     SDLLSLARIINER"
     gene            complement(3231..>3565)
                     /locus_tag="VFX11_08570"
     CDS             complement(3231..>3565)
                     /locus_tag="VFX11_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938713.1"
                     /translation="TGANGVRGYEPALFSADFAWKLSLEQSLMSFAPTAGTTLVPFLF
                     ADYAEGEQNTDFGITASFTAAGLGLDAYYGTSLNARLTAGFPLQEKLETGQEEFDPVV
                     FGYITYSF"
BASE COUNT          738 a          968 c         1012 g          847 t
ORIGIN      
        1 gtccagcggc ggtaccgcgg cggctgttgc cgccggcatt gtgccgttcg gttctgcagg
       61 cgacggtggt ggttcaatcc ggataccctc tgcctgctgc ggtctgttcg ggctgaagcc
      121 cagtcgggga ctggtgccgt cgggacctga ttatggtgaa atctgggacg gagcgtcggt
      181 ggagcatgtg atcacccgaa gtgtgcgcga ttctgccgct atgctggatg ttctggcagg
      241 cagtgatgcc gcgtcgcatg tggcgctccc cttcaaaaca gacagctttc tgcaggcgct
      301 ggaacaacct ccgcccaaac tgaaaatcgg gttcagtacg gagtcgccca tgggcggaac
      361 ggtggctgtt gaatcgcgtg atgctgtgcg caacgtggcc gaactgctgc aatcgcttgg
      421 gcatgtggtg gagcaggcgg caccggcggg aatcgatgcc agccggcttt cccgcgccta
      481 tgccgatatt tatctggccc acgtactggc ggacgtgttg caggttgagc gtgctcatgg
      541 gcgatgctat gcggcacgta atttcgagcc agccacactt ttcatcgcca atctgggacg
      601 acgcttttct gccggcgcgt atttgcgttc acggcagtac tgggttcagc tgcgccgtct
      661 catggcggat tttcataagc gttatgatct gtggctgaca ccggttctgg cggccgagcc
      721 ctatcgtctg ggcgaactgc ggtccagtcg tgtggaggag caggcaatgg atgttctcaa
      781 tcacaccggt ctgcatcgcc tggtgccgat ggcgcaatac tatcgcatca cgttgcagca
      841 gttgcggcgg gtacctttta cgcagatcgc caatctcacc gggcaaccgg cgatgtcggt
      901 accgctggta tggagtcaca aggggctgcc gatgggcgtg cagctggtgg gtccggttgg
      961 ctcagaccgg cgcctgttgc agctggcacg acaactcgaa caggcccgcc cctggtttgg
     1021 ccggcagcca cagttgtaaa gggcgggaat cttcagaaag cgtgcctttc accgttatgt
     1081 tatggcgaat tcgtcgcggt tggtgctgtc ctgcagttcg gtctttttca gttctgaccg
     1141 gatcagatcc ttgagccggt tttcgctgac cggaccctgc tcgataaagg catccagcat
     1201 tcttttcacg cgtaccagat gccactcgat ttcatccagc atcaacgaca cgtcatcgat
     1261 ctcgcggtaa gcaaatgaca gtcggtcctt gcgcacctgc tgcagccagc gcaggtatcc
     1321 cgcgtccaga atgcccgatc cgcgcacgat acgcatcacc tgtatcagca ctgttttcgc
     1381 gctggtaagg agtttgtggt ttacgccttc cttgaacaga cccaccacct gctccaggct
     1441 gttgagcagc aggctcaggc gatcattgtc gcgtgtcagc ttgtcgatct gcgcatccag
     1501 ggattcattg gtggtgaaag gggattcatc caggtcggca ttgcgcagaa tcaggatcaa
     1561 tgccttgtag gtgtcataga aatggctttc gctgttgacc tgcagcagcg gcatcagatc
     1621 acgcagattg cgaccgttgg gaagcaggcg cttggtgaac actgcgatga cactgtccga
     1681 caaagcgatg acctgacctt caatgcaaag atggtgcgcg aattttcctg ccggataacc
     1741 cgagccatcg cagcgttcgt gatgctcgct gacagccagc gcaacccgtg gatccatgcc
     1801 ggaaatacgt tccagtactt tctgggcgat aatggtatgt gcttgaatct ggcgccactc
     1861 tgcaggtgtg agcgtatcct gcttgttcac caccagcgga tcgatgtgca tcatgccgat
     1921 gtcgtgcgcc agcgcggcca gaaaacaggt ctcgatctgt tgtgccgtta atttcatgtt
     1981 atgagcgatc accaccgaca gccaggcaat acccagcgac tgccggtaca gctggttcat
     2041 ctgttccttg agtacggtaa gcttctgctt caggatcgcg aactttgcgt atgacgagca
     2101 cagctgcttg atggcgctat cgatctcgat ccgctggtga accagcttga tctgcggata
     2161 gcggtccaga atttccatca ggtcgcggta gaggtccacc gcactcaggc tgttgttgat
     2221 gtcgacactg gcttcaatgg gcttgatcag cttgaagcga atgattttgt ccgccattcg
     2281 ctcgttgata ggggatcctt tcttgatgag caggatgccc tgatcgttgt aaatatcttc
     2341 ggaggcgaca atttcccttt tttcaccaag ttgggtcaat ctttgcgcgt acgctttgtc
     2401 gtgcaaggga tcgaagtcgg ccatgtcctg ctactgtaaa atcgggaagg gggattaatt
     2461 ttaaattgaa ttaaatttag ccagagatcg gccgataagc aatgcaaaaa tagcctcttt
     2521 tttgtcgcgc cacatgtctg ctcgagtcaa attaccggcg ctaatagcaa caacgggtta
     2581 gcttggccat cctgcccaac gggtggagac gtgcaggttg ttgacgcagt ctgccagact
     2641 gaattgtcag cgttcattga tgatgcgcgc cagtgacagc aggtcggagt tgatcagcag
     2701 accgctttcc atggctttgg acagattgat ctcgaattgt tgctgatcgc gggtgcgcag
     2761 tatgttcacc acgccgcctt ttctcgcgaa gtcctcggta ttgccaatgc tgagcacaaa
     2821 cagatggttg aaatccctga aaaaagattc gagctcgccg acatggtcga tgcccacgaa
     2881 cagtatgtga cagccggttt tgattttggc gtaatcggtg gtgcgtatta cctgcaggaa
     2941 ccggctccga atctgggccc cattcaccac atccaaaaat tctccgaatg gcacagcgcc
     3001 gtaaagacac atcttcagtg gactacccgg attctcaaag gcattgtccg gccactccac
     3061 gaaattggcg aaattgtaaa gaaacagggc cttgacctga tactccttca ccagctcaag
     3121 atcggtatct gcacgcgtca atgtcgtggt cagcaacagc agaaccatgc ccactacggg
     3181 caggatcttt ctgtctctgt gacgtgcgtt aaaaccagtc atcgacctgc ctaaaaactg
     3241 taagtgatat aaccgaaaac caccgggtca aattcttcct gacccgtttc aagtttttcc
     3301 tgcagaggga agccagcggt cagccgtgca ttaagcgaag tgccgtagta ggcatccagt
     3361 cccagtccgg cggccgtgaa gctggccgtt ataccaaaat cggtgttctg ctcaccctca
     3421 gcatagtcgg caaacaggaa cggcacgagc gtcgttcccg ccgtcggcgc aaacgacatc
     3481 agggactgct cgaggctcag tttccacgca aaatctgcac tgaacaatgc gggctcgtag
     3541 ccgcgaacgc cgttggcgcc cgtca
//
LOCUS       DASUEG010000459         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_252013, whole genome shotgun sequence.
ACCESSION   DASUEG010000459 DASUEG010000000
VERSION     DASUEG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252013"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2580)
                     /locus_tag="VFX11_08575"
     CDS             complement(52..2580)
                     /locus_tag="VFX11_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938714.1"
                     /translation="MEMLQKLTGTQRNWLLAGLPTLALLVFVAGSWNIIQAGIATQGG
                     SVTGVLLVAALLVAASLAMLLTGAGSVDRDNERLQRSLEVCQANVMVADNDFNIRYMN
                     ESVRDMMQRNEARLREAIPGFDARNLVGMNVDTFHKNPSHQRRLVSGLNTAYKTNLTL
                     NGLTFGLIATPIMSEKGERIGTVVEWYDKTAELAKQAAEEKIAGENARIAQALRICDT
                     SVMMADTDLNIVYMNDAVVRMMREADSELKKVIPAFDSSRLLGANVDMFHKNPAHQRK
                     MLANLREPYSTNIKVGSLTFGLIATPVNDDKGNRLGTVVEWKNKTIELAKAEQERRVA
                     EENQRVRQALDNVTTNAMIADRDGQIVYMNKSVREMLQVAESDIRKEMPHFDSGKLLG
                     ANFDRFHKNPAHQRRLLENLTSTYTAQISLGGRTFRLVANPVMDEAGGRIGTVVEWAD
                     RTEEVRTEREIDHLVSSAAGGNLTVRLEEAGKSGFMLNLSKGLNSLMNATEQVVTDTI
                     RVLDALAHGNLNERIEREYQGSFGKLKSDANATVDRLIDIITGIVESANAVSNGADEI
                     AQGNSDLSQRTEEQASSLEETASSMEEMTSAVRQSSENAKEANSLATLARDKAARGGE
                     VVAHAVAAMGAINASSKKIADIISVIDEIAFQTNLLALNAAVEAARAGEQGRGFAVVA
                     GEVRNLAQRSAGAAKEIKELIRDSVGKVDDGTRLVNESGETLRGIVDSVQKVSSMIQE
                     ITAAALEQTSGIEQVNTAIAQMDEMTQQNAALVEEASAAGEALSEQARQLMSLMSFFS
                     VENVARPGVPGPQAVRSPQVSAAAGRSQARVASARRSNDEWEEF"
     gene            complement(2626..3186)
                     /locus_tag="VFX11_08580"
     CDS             complement(2626..3186)
                     /locus_tag="VFX11_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011863921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938715.1"
                     /translation="MNSAEQRAHEIADADVSIRSRDQNDQFLTFIMAGEEYGVDILQV
                     QEIRGWENVTPIPNAPAHIKGVINLRGTIVPIVDLRQRFGLQRADYGPVTVVIVLKVA
                     TRKGSRVMGIVVDAVSDVYSLSADDMKDAPDLGDNVNTSYIRGLVNVSSKMVILLDIN
                     ELLGADTIPEVSVSALQELKNRSDQH"
     gene            complement(3211..>3713)
                     /locus_tag="VFX11_08585"
     CDS             complement(3211..>3713)
                     /locus_tag="VFX11_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010557758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4938716.1"
                     /translation="FTIRLPLTLAILDGQLIQVGEQVYIIPLVSIVESLQPDLALVNR
                     VAGGCDVFRLRNEYIPIIRLAEIFGVVPRNEGLEKCLLVVVETGNAKVALVVDDLLGQ
                     QQVVIKSMEANYRKVEGISGATILGDGTVSLILDITDLIQMAGVRQQLKSGLKLVSDR
                     SRVAIR"
BASE COUNT          700 a         1174 c          992 g          847 t
ORIGIN      
        1 atgccgccgg atgcctcctg ctgcgcgggt gaagcgacgt ccattgcgtt ttcaaaattc
       61 ttcccactcg tcgttgctgc gccgggcgga agcaacccgc gcctggctac ggccggctgc
      121 ggctgacact tgcggcgacc gcacagcctg gggaccgggt acacccgggc gcgccacatt
      181 ttccaccgag aagaaactca tgaggctcat cagttgcctg gcctgctccg acagggcctc
      241 gccggcggcg gaagcttctt ccaccagcgc cgcattctgc tgcgtcatct cgtccatctg
      301 cgcaatcgcg gtgttgacct gctcgatgcc cgaggtctgc tccagcgccg ccgccgtgat
      361 ttcctgaatc atggacgata ccttctgcac cgaatcgaca atgcctcgca gggtttctcc
      421 ggattcgttc accaggcggg taccatcatc caccttcccg acgctgtcgc ggatcagctc
      481 cttgatttcc ttggcggcac ccgcagaccg ctgcgccaga tttcgcactt cacctgctac
      541 cacggcaaaa ccgcggccct gctcgccggc acgggcggct tccaccgctg cattcagcgc
      601 caacagatta gtctggaatg cgatttcatc gatcacactg ataatgtctg caattttctt
      661 gctggaggca ttgatcgctc ccatcgccgc cactgcgtga gccaccactt caccgccgcg
      721 tgcagccttg tcgcgggcga gcgtggcaag cgagttagcc tccttggcat tttcgctgct
      781 ctggcgcacg gcgctggtca tttcttccat gctggacgcg gtttcctcca gtgaagatgc
      841 ctgctcctcg gtacgctggc tgagatcgga attgccctgc gcgatttcgt cggcaccgtt
      901 ggacacggca ttggcgcttt ccacaatacc ggtgatgatg tcgatcaggc gatccaccgt
      961 ggcgttggca tcggatttga gcttgccaaa actgccctga tattcgcgct ctatgcgttc
     1021 gttcaaattg ccgtgcgcca gcgcatccag tacgcggatg gtatcggtca ccacctgctc
     1081 cgttgcgttc atcaggctgt tgagcccttt actcagattc agcatgaaac cggatttgcc
     1141 agcttcttcc agccgcacgg tcagattgcc tcctgccgcc gacgaaacca gatgatcgat
     1201 ttcgcgttcg gttctgactt cctctgtgcg atcggcccat tccaccacgg tgccaatccg
     1261 gccacccgct tcgtccatca ccgggttggc caccagcctg aacgtacgac cacccagtga
     1321 aatctgcgct gtgtaagtgc tggtgagatt ttcaagcaat cggcgctggt gggcgggatt
     1381 tttgtggaaa cggtcgaagt tggcgcccag cagtttgccg gaatcgaaat gcggcatttc
     1441 cttgcgaata tcggattccg ccacctgcaa catttcccgc accgacttgt tcatgtaaac
     1501 gatctggcca tcccggtcgg caatcatggc attggtggtc acattgtcca gcgcctgccg
     1561 cacccgctgg ttttcttccg caacgcgacg ttcctgctcg gccttagcca gctcgatggt
     1621 tttgtttttc cactccacca cggtgcccag gcggttaccc ttgtcgtcat tcaccggcgt
     1681 ggcgatcagg ccgaacgtga gcgaaccgac ctttatattg gtgctatagg gctcgcgcag
     1741 gttggccagc atcttgcgct ggtgggccgg atttttatgg aacatgtcca cgttggcgcc
     1801 cagcaggcgg gaagaatcga aagcgggaat cactttcttc agctcggaat ccgcttcgcg
     1861 catcatgcgg acaacagcgt cattcatgta cacaatgttg agatcggtat cagccatcat
     1921 cacactggtg tcgcagatac gcagggcctg cgcaattcgg gcattctcac cggcgatctt
     1981 ttcctccgct gcctgttttg ccagttccgc cgtcttgtcg taccactcca ccacggtgcc
     2041 gatgcgctcg cccttctccg acatgatggg cgtggcaatc aggccgaacg tcagcccgtt
     2101 gagggtaagg ttggttttgt acgcggtgtt cagacccgat accagacggc gctggtgcga
     2161 agggttcttg tgaaaggtat cgacattcat gcctaccaga ttgcgggcat caaagccggg
     2221 aatggcttcg cgcaagcggg cttcgttgcg ttgcatcata tcacgcacgg attcattcat
     2281 gtagcggata ttgaaatcat tgtccgccac catcacattg gcctggcata cctccaggct
     2341 gcgttgcaga cgttcgttgt cgcggtcgac actacccgcg ccggtcagta gcatcgccag
     2401 gctggcggcc accagcagtg cagccaccag caatacgccc gtgacactgc caccctgcgt
     2461 cgcgataccg gcctgaatga tattccagct gcctgcaaca aacactaaga gagccaacgt
     2521 cggcagtcct gccagtagcc agttgcgctg ggtgcctgtc agcttctgta acatttccat
     2581 tgtgattcct cgccttttat ccagttgcca ctgccaacgc gccgatcaat gctgatcgga
     2641 ccgattcttc aattcctgca acgcagacac cgatacctcg ggaatcgtgt cggcgccgag
     2701 cagttcgttg atgtccagca ggatcaccat cttgctggac acattcacca ggccacggat
     2761 gtatgaggta ttgacgttgt cacccagatc cggagcgtcc ttcatatcat cggcagaaag
     2821 gctgtacacg tcgctgaccg catccaccac gatgcccatc acccggctac ccttgcgggt
     2881 tgccactttc aacacgatga ccacggttac aggcccgtaa tcagcccgct gcagaccgaa
     2941 gcgctgacgc agatcgacga tcggtacaat ggtgccgcgc agattgatga cacccttgat
     3001 gtgtgcgggc gcgttcggaa tcggcgtgac gttttcccag ccacggattt cctgcacctg
     3061 caggatgtca acaccgtatt cctcacctgc catgatgaag gtaaggaact gatcgttctg
     3121 atcgcgggac cggatcgaaa cgtcggcgtc cgcgatttcg tgcgcccgtt gttctgcgct
     3181 gttcatgact gctctcctgt tcggatcatg tcaccgtatg gcgacgcgac ttctgtctga
     3241 aaccagtttc aaaccacttt tcagctgctg gcgcacgccg gccatctgga tcaggtcggt
     3301 gatgtccaga atcagcgaca ccgtgccgtc ccccagaatg gtggcgccgg aaatgccttc
     3361 caccttgcgg taattggctt ccatgctctt gatcaccacc tgctgctgac ccagcagatc
     3421 atccaccacc agtgccacct tggcattgcc ggtctccacc accaccagca ggcacttttc
     3481 cagaccttcg ttacgcggta caacgccgaa gatttctgcc agccggatga tgggaatgta
     3541 ttcgttgcgc aggcggaaca catcacagcc accagccacc cggttcacca gcgccagatc
     3601 cggctgcagc gattcaacaa tggacaccag cgggatgatg taaacctgct cgcccacctg
     3661 gatcagctgt ccatccagaa tggccagggt gagtggcaga cggatcgtaa agg
//
LOCUS       DASUEG010000460         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1892177, whole genome shotgun sequence.
ACCESSION   DASUEG010000460 DASUEG010000000
VERSION     DASUEG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892177"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFX11_08590"
     CDS             complement(<1..1322)
                     /locus_tag="VFX11_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4938717.1"
                     /translation="MDTIQIRGARTHNLKNIDLDLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSTYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLFARVGTPRCPDHDLALEAQTVSQMVDQVMTLPEANRYMLLAPVIKEKKG
                     EHLHLFEELKANGFIRARINGIVVDLDQAPKLDKNKKHTIEVVVDRFKVKSGMELRLA
                     ESFETALNLAGGTAEVMNMENEKDQHLFSSKFACPVCNYSLSELEPRIFSFNNPAGAC
                     TACDGLGVTQFFDEETIVTSAELSLTEGAIRGWDKRSIYYYQMLTSLAKHYGFDIDKP
                     YGKLPKKIRQLILHGTGDESVPFRYLNDRGDHITRNHPFEGIIPNMERRYRETESDAV
                     REELAKHLTTAECQTCHGSRLNRSARHVYIEGTTLPDVIRKPVNDSLT"
     gene            1490..>2500
                     /locus_tag="VFX11_08595"
     CDS             1490..>2500
                     /locus_tag="VFX11_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938718.1"
                     /translation="MNALEARATWSLASLFVFRMLGLFMVLPVFALYGQDLVGATPFL
                     LGLAIGAYGFSQALLQVPFGYLSDRVGRKPMILVGLALFCAGSVVAALSTSITGVVIG
                     RILQGTGAIAGPVMALLSDVTREDQRTKAMATVGASIGLSFSLALIAGPMIAGWFNLG
                     GVFWLTAVLAVVGMALTLWVVPDPARPPARDLRPAAAQFRVVLGQVELLRLNLGVFLL
                     HMSMTSLFVVLPVLLVERAHVEKSAHWQIYLPILFVSFVLMLPLMIAAERKRKVKQVF
                     LTAIVLLVTALLMLANWHGSLLAIVAGLLVYFWAFNLLEAMLPSLVSKVATPALKGTS
                     MGV"
BASE COUNT          400 a          672 c          827 g          601 t
ORIGIN      
        1 taggtcagtg agtcgttgac cggcttgcgg atcacgtccg gcagggtggt gccttcgata
       61 tacacgtggc gcgcggaccg gttcaggcgc gagccatggc aggtctggca ctcggcggtg
      121 gtgaggtgct tggccagttc ctcgcgcacg gcgtccgact cggtttcccg gtagcgccgt
      181 tccatgttgg gaatgatgcc ctcgaagggg tggttgcggg tgatgtggtc gccgcggtcg
      241 ttcaggtagc ggaagggaac ggattcgtcg ccggtgccgt gcaggatcag ctggcgtatc
      301 tttttcggaa gcttgccgta gggcttgtcg atatcaaaac cgtaatgctt ggccagcgac
      361 gtcagcatct ggtagtagta gatgctgcgt ttgtcccagc cgcgaatggc gccctcggtg
      421 aggctcagtt cggcggaagt gacgatggtt tcctcgtcga agaactgggt gacgcccagg
      481 ccatcgcagg cggtgcaggc gcccgccggg ttgttgaagg agaagatgcg cggttccagt
      541 tcgctcaggc tgtagttgca taccgggcag gcgaacttcg aggaaaacag gtgctggtcc
      601 ttttcgttct ccatgttcat gacttcggcg gtgccgccgg ccaggttcag tgccgtttcg
      661 aaggattctg ccagccgcag ctccatgcca gatttcacct tgaagcggtc caccactact
      721 tcgatggtgt gcttcttgtt cttgtccagc ttgggtgcct ggtccaggtc caccacgatg
      781 ccgttgatgc gggcgcggat gaagccgttg gccttcagtt cctcgaacag gtgcaggtgt
      841 tcgcctttct tctccttgat caccggcgcc agcagcatgt agcggttggc ttccggcagt
      901 gtcatcacct ggtccaccat ctggctgacg gtctgggctt ccagtgcgag atcgtggtcc
      961 gggcagcgcg gcgtgccgac ccgggcgaac agcaggcgca ggtagtcgta gatctcggtg
     1021 atggtgccga cggtggagcg ggggttgtgc gacgtgcttt tctgctcgat ggaaatcgcc
     1081 ggggagagcc cttcgatatg atccacatcc ggtttttcca tcagggacag gaactggcgg
     1141 gcataggtgg acagcgattc cacgtagcgg cgctggcctt ctgcatagag ggtatcgaac
     1201 gccagcgatg atttgccgga accggacaac ccggtaatga cgataaattt gtcccttggc
     1261 aggtccaggt cgatgttttt aagattgtgg gtgcgtgcgc cgcgaatctg gatggtgtcc
     1321 atgcgtgttt ccccctgcaa aggcaaaccg taaagtataa agactcgggg tgacagcgac
     1381 tagtttgaat ctttgtatga atcaatggtc attgaggttg ctacgagcgc agtcaacata
     1441 atgttaatat ccgtgcccac tccattcttt ggttgagaat ccacactgca tgaatgcttt
     1501 ggaagcccgc gccacctggt ccctggcaag cctgttcgtg ttccgcatgc tgggactgtt
     1561 catggtgctg ccagtatttg ccctctacgg gcaggatctg gtgggggcga caccatttct
     1621 gctgggcctg gcaatcggtg cctatggctt cagccaggcg ctgctgcagg tgccattcgg
     1681 ctatctgtcg gaccgggttg gccgcaagcc gatgatcctg gtgggcctcg cgctgttttg
     1741 tgccggcagc gtggtggcgg cactttcgac gtcgataacg ggagtggtca tcgggcgcat
     1801 tttgcaggga accggcgcca ttgccggccc ggtcatggcg ttgctgtcgg acgtcacccg
     1861 cgaagatcag cgcaccaagg ccatggcaac ggtgggggca agtatcggcc tgtcgttttc
     1921 attggcgctg attgcggggc cgatgatcgc cggctggttc aatctgggtg gcgtgttctg
     1981 gctgaccgcg gttctggccg tggtgggaat ggcattgacc ctgtgggtgg tgcctgatcc
     2041 agcccgcccc ccggcacgcg acctgcgtcc tgccgccgcg cagttccgcg tggtgctggg
     2101 ccaggttgaa ctgctgcgcc tcaatctggg cgtgtttctg ctgcatatga gcatgacctc
     2161 gctgttcgtt gtgctgcccg tgttgctggt ggagcgggcc catgtggaaa aatccgcgca
     2221 ttggcaaatt tatctgccga ttcttttcgt gtcgtttgtc ttgatgttac cgctgatgat
     2281 cgcggcggag cgcaagcgca aggtcaagca ggtgttcctg accgccattg tcctgctggt
     2341 gactgcgtta ttgatgctgg ccaactggca tggttcactg ctggccatcg tcgccgggct
     2401 gctggtgtac ttctgggcct tcaacctgct ggaagccatg ctgccttccc tggtgagcaa
     2461 agtggcaaca ccggcgctga aaggcacctc catgggggtg
//
LOCUS       DASUEG010000461         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_155292, whole genome shotgun sequence.
ACCESSION   DASUEG010000461 DASUEG010000000
VERSION     DASUEG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155292"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="atpG"
                     /locus_tag="VFX11_08600"
     CDS             <1..617
                     /gene="atpG"
                     /locus_tag="VFX11_08600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX4938719.1"
                     /translation="DKGLCGGLNTNALRLALNKMRGWQDQSIEVDVCAIGSKGLQFFQ
                     RIGGRIVAKMTHLGDTPHLEHLIGPVKVMLDAYTAGKIDELHIVYTRFFNTMKQEAQC
                     MQLLPVPAGELVTEGGHSWEYIYEPDAKTVVDGLLVRYVEAIIYSAVAENMASEQSAR
                     MVAMKAASDNAGKVIDELKLVYNKTRQAAITKELSEIVSGAAAV"
     gene            617..2074
                     /gene="atpD"
                     /locus_tag="VFX11_08605"
     CDS             617..2074
                     /gene="atpD"
                     /locus_tag="VFX11_08605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4938720.1"
                     /translation="MASAQRLSISGINMSQGKIVQIIGAVVDIQFDRGAMPKVFDALQ
                     LADGELVFEVQQQLGDGVVRAIAMGSTDGLKRGLAVVNTGNPISVPVGAATLGRIMDV
                     LGNPVDEVGPVVSDIKWSIHRKAPSFDELSPTTELLETGIKVIDLICPFAKGGKVGLF
                     GGAGVGKTVNMLELINNIAKAHSGLSVFAGVGERTREGNDFYHEMADSGVINMENLPE
                     SKVAMVYGQMNEPPGNRLRVALTGLTMAEYFRDEKDASGKGRDVLFFVDNIYRYTLAG
                     TEVSALLGRMPSAVGYQPTLADEMGRLQERITSTKHGSITSIQAVYVPADDLTDPSPA
                     TTFAHLDATVVLSRDIAALGIYPAVDPLDSTSRQLDPQVVGEEHYGVARAVQQTLQRY
                     KELRDIIAILGMDELSAEDKLIVARARKIQRFLSQPFHVAEVFTGAPGKYVPLKETLR
                     GFKGIVSGEYDHLPEQAFYMVGTIDEAVEKAKNLS"
     gene            2087..>2442
                     /locus_tag="VFX11_08610"
     CDS             2087..>2442
                     /locus_tag="VFX11_08610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX4938721.1"
                     /translation="MAMTMHVEVVSAERSLFSGTAELVVAPADGGEVGILARHAPMLL
                     RIKPGAVRIKLSLQEQEEVLYVSGGYMEVQPHSVTILSDTAVRAGDLDESKALEAVRL
                     AEEAMNDKASAIDYARA"
BASE COUNT          502 a          721 c          740 g          479 t
ORIGIN      
        1 ccgacaaggg cctgtgcggt ggtctgaata ccaatgcgct gcgccttgct ctgaacaaga
       61 tgcgcggctg gcaggatcag tcgatcgaag tggacgtctg cgcgatcggc tccaagggtc
      121 tgcaattctt ccagcgcatt ggtggccgca ttgttgccaa gatgacccat ctgggcgaca
      181 cgccgcacct ggaacacctg atcggaccgg tcaaggtcat gctggacgcc tatacggccg
      241 gcaagatcga cgagctgcat attgtgtaca cccgcttctt caatacgatg aagcaggaag
      301 cccagtgcat gcagctgctg ccggtacccg caggtgagct ggtgaccgaa ggtggccata
      361 gctgggaata catttacgag ccggatgcca agacggtggt tgacgggctg ctggtgcgtt
      421 acgtggaagc gatcatttat tccgccgtgg cggaaaacat ggcttccgaa cagagcgcac
      481 gcatggtggc catgaaggcc gcgtccgaca atgccggcaa ggtgattgac gagctgaaac
      541 tggtctacaa caagacccgc caggccgcga ttaccaagga attgtccgaa attgtatcgg
      601 gcgccgctgc cgtttgatgg caagcgcaca acgtttatcg atatcaggaa taaacatgag
      661 ccaaggaaaa atcgtccaga tcattggcgc cgttgtcgac atacagttcg accgtggtgc
      721 catgcccaag gtgtttgatg ccttgcagct tgctgacggc gagctggtat ttgaagtgca
      781 gcaacagttg ggcgacggcg tagtacgtgc catcgccatg ggctccaccg acggtctgaa
      841 gcgcggcctg gccgtggtga acaccggcaa ccccatttct gtcccggttg gcgccgccac
      901 cctgggccgt atcatggacg tgctcggtaa cccggttgat gaagtcggtc cggttgtctc
      961 cgacatcaag tggtccattc accgcaaggc cccgagcttt gacgaactgt cccccacgac
     1021 cgaactgctg gaaaccggca tcaaggttat tgacctgatc tgcccgttcg ccaagggtgg
     1081 taaggtgggt ctgttcggtg gtgccggcgt gggcaagacc gttaacatgc tggaactgat
     1141 caacaacatc gccaaggccc actcgggtct gtccgtgttt gccggcgtag gtgagcgtac
     1201 ccgtgaaggt aacgacttct accacgaaat ggccgactct ggcgttatca acatggaaaa
     1261 cctgccggaa tccaaggtag ccatggtgta cggccagatg aatgagccgc cgggcaaccg
     1321 tctgcgcgta gcgctgaccg gtctgaccat ggccgagtac ttccgtgacg aaaaggacgc
     1381 ttccggcaag ggccgtgacg ttctgttctt cgtggacaac atctaccgct acacgctggc
     1441 cggtaccgaa gtatccgctc tgctgggccg tatgccgtcc gccgtgggct atcagccgac
     1501 gctggccgac gaaatgggtc gcctgcaaga gcgtattacc tcgaccaagc acggttccat
     1561 tacgtccatc caggccgtat acgtacctgc cgatgacttg accgacccgt ctcccgctac
     1621 caccttcgcc cacctggacg ctaccgtggt tctgtcgcgt gacatcgctg ccctgggtat
     1681 ctacccggcc gttgatccgc tcgactccac ctcgcgccag ctggacccgc aagtggttgg
     1741 cgaagagcac tacggtgttg cccgtgccgt gcagcagacc ctgcagcgct acaaggaact
     1801 gcgcgacatt atcgccattc tgggcatgga cgaactgtcg gccgaagaca agctgatcgt
     1861 ggctcgcgcc cgtaagatcc agcgtttcct gtcgcagccg ttccacgttg ctgaagtatt
     1921 taccggcgct cccggcaagt acgttccgct gaaggaaacc ctgcgtggct tcaagggcat
     1981 cgttagcggt gaatacgacc acctgccgga gcaggctttc tacatggtcg gtaccatcga
     2041 cgaagctgta gaaaaagcca agaacctgtc ctaaggggga ttcgccatgg cgatgaccat
     2101 gcacgtggaa gtagtcagcg ccgagcggtc cctgttctcc gggaccgccg agctggtggt
     2161 ggctccggct gacggtggcg aagtcggcat tctggcgcgt cacgcgccga tgctgctgcg
     2221 catcaagccg ggtgccgtgc gcatcaagct gtccctgcag gaacaggaag aagtgctgta
     2281 cgtatcgggc ggctatatgg aagtgcagcc gcactcggtg accatcctgt cggacaccgc
     2341 cgtacgcgcc ggcgatctgg acgaaagcaa ggccctcgaa gccgtgcgtc tggccgaaga
     2401 agcgatgaac gacaaagcgt ccgccatcga ctacgctcgc gc
//
LOCUS       DASUEG010000462         6417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2011531, whole genome shotgun sequence.
ACCESSION   DASUEG010000462 DASUEG010000000
VERSION     DASUEG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011531"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4569
                     /locus_tag="VFX11_08615"
     CDS             <1..4569
                     /locus_tag="VFX11_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4938722.1"
                     /translation="LLFAGQARMAAIVESSADGIIGKSLDGTILSWNRGAEQIFGYHA
                     SEAIGQSVTSLLVPEDLKNEEATVLAKIARGEQVLNVKTRRRHRDGHLVDVSITVSPL
                     RGPHDSVIGVSTTVRDISEQQAAEARIHELNANLEAQVQLRTAELNQLNLLMNSVLRA
                     ATEVSIIATGLDGKISLFNRGAERILGCAAEDIVGKSTTERFFVPEELAARSKELSLE
                     TGRPIEGRQALILKPEIEGAESREWTLQRQDGSRFIASVAITAMRDNDGQLTGFLGIA
                     IDITRQKNTERELASSLATTQAILDTAPNPIITVDDKGQVLSFNPAGERVFGYGQNEI
                     VGQNVRLLMPDSNRPDHANLFAHYRRDDNSAVVDTGREVLARHKDGTVFPIHLAVGTM
                     TFAGTRRYVGLITDLTEQYQQRMALLAARDQLMMAANVARLGIWTWTLADNSLQWNDR
                     MFELYDYPLSLRETGLNYSHWRQRLHPDDRQANDQSLSAALEGRGNYDIIFRIILPDG
                     RVRFLQVAAYIERDERNQPVKVTGINLDITAQRELETRLLQAKEQADLASAAKSSFLA
                     NMSHEIRTPMNAVLGLLQLVRQTELNLRQQDYVDKALSSGRALLSLLNDILDYSKIEA
                     GKLELDLHPLELDALMRELGDVLSGNQGKKDIEILFAIDPNLPSVLVGDKLRLLQVMI
                     NLAGNAFKFTQQGQIVIGVEELQRSNGSILLRFSVTDTGIGISTEQLQRIFQSFTQAE
                     ASTARRFGGSGLGLVISKRLIELMHGTLEVTSEPGKGSRFWFDVPLGVANFTPMRESC
                     PTADVPLHVLIVDDNPVAAELMTATARALGWRTQTAFSGNDALARVRDQASHNDRFDL
                     VLMDWRMPDMDGLDAAKQIRNSSTTPGSPLVIMVSAYEREAMASEQQHADAPFADFLT
                     KPVTPRQLANTVMQALTGTSATPAAGVEDKPRTQRLRDLRLLVVDDNAVNRQVVAELL
                     QGEGASVTLAEGGAEGVAAVHANPDTFDAVVMDMQMPDVDGIEATRRIRADARFSRLP
                     IMAMTANASTADRDACLAAGMNAHVGKPIDLEQTVATLQTLTGRRTLQPVTLPPPPID
                     TQGPLVESLAAIMSRLGGKRALYACMLTLFEPEENSLIDELAAKITHQDIAGSAATLH
                     AIKGSAGTLGATALAQHAAALEFRLKSGDATAVEAVMSPATVQTLRHLLTESVRALNA
                     ELAGDTAHPTTTAVQSAPSPRAKILIVDDQPLNISILNELFRDEYELHSATSGEQALA
                     LCASQQPDLVLLDVMMPGMDGHDVCRQLKADPLTSTIPIIFITGQVDEAEEIMALELG
                     AVDFITKPINAVVVMARVKTHIALKRQGDLLRSNALLDSLTRIGNRRRFDADLQIFWR
                     QCFRERTPLSLILIDVDFFKKYNDRYGHVLGDACLVSVAQALEHCCQRPFDSLARYGG
                     EEFACLLPMTDLAGATAIAQAMQTQVRNLNIEHLDSTTASVVTISLGVATLIPASDIS
                     PEQLTELADQRLYAAKTAGRGQVCAAAP"
     gene            complement(4588..5442)
                     /locus_tag="VFX11_08620"
     CDS             complement(4588..5442)
                     /locus_tag="VFX11_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938723.1"
                     /translation="MITEEIRVQVNGMELAAKQWGDPELPAIIALHGWLDNAASYDRL
                     SPALTQHRVIALDFAGHGYSGHRPEGVRYHTIDNVDDVIGLADALGLQEFILMGHSMG
                     AGIATFTAGSFPERVTKLILIEGIGSNTNPAEDAPRVLRKAVEDMKKTSAKRKPVYET
                     RDDAIHTRAHAIGVISVDASTHICNRGLMPAGEGFTWRSDPRLKMSSAIRLTEDMVTA
                     YLQKLTMPVLLVRGRHSFFATEKTLQNRADKIPGCQQVLLDGNHHLHLEAETFAPVAE
                     AINGFLAA"
     gene            complement(5439..6218)
                     /locus_tag="VFX11_08625"
     CDS             complement(5439..6218)
                     /locus_tag="VFX11_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938724.1"
                     /translation="MLVHFAHANGVPAAAYQPIFDRLQPHTVIALPMFGHNPAFPCTH
                     NWRYLADELIDFLQRNAQEPVLGVGHSMGAVVTFIAACKRPELFRGVLMLDPPLMWGR
                     MAMVFKVLKLLGKSDDFTPAGKSKNRKQSWPNRANAIEYFASKRLFQFHPECFESFCN
                     AAIDQSEGDQVKLRFRVDVEVEIFRNTSDNLKSCKRPPQLPMKLVYADRSDASRADMV
                     VPFARHFSIPLEQIPGEHMYPLQQPDVAAELIHQFIRECQP"
     gene            complement(6279..6416)
                     /locus_tag="VFX11_08630"
     CDS             complement(6279..6416)
                     /locus_tag="VFX11_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX4938725.1"
                     /translation="MFERLRVYLNRSGNLVAVGALHLPGEHGLLQLLRKAGYRVQPVR
                     P"
BASE COUNT         1387 a         2029 c         1835 g         1166 t
ORIGIN      
        1 ctgctgtttg ccggtcaggc ccgcatggct gccattgtgg aaagttccgc cgacggcatc
       61 atcggcaaat ccctcgacgg taccattctg tcgtggaacc ggggcgccga gcagattttc
      121 ggttaccacg ccagtgaagc catcggtcag tccgtcacct cactgctggt gccggaggac
      181 ctgaagaatg aggaggccac cgttctggca aagattgccc gcggcgaaca ggtcctcaat
      241 gtcaaaacac ggcgccgcca ccgcgacggc catctggtgg atgtctccat caccgtatca
      301 ccgttacgcg gtccgcacga ttcggtcatt ggcgtatcca ccaccgtgcg ggatatctcc
      361 gaacaacaag ccgctgaagc ccgcatccac gaactcaatg cgaatctgga agcccaggtg
      421 caactgcgca cagcagaact gaaccagctc aacctgctga tgaacagcgt actgcgcgcg
      481 gctaccgaag tatcgatcat cgccacagga ctggatggca aaatcagtct gttcaaccgc
      541 ggcgccgaac gcatactggg atgtgccgct gaagacatcg tggggaagtc cactaccgag
      601 cgcttctttg tccctgaaga actggcggcc cgcagcaagg aactgtcgct ggaaacaggc
      661 aggccaatcg agggtcgcca ggcgctgatc ctgaaacccg aaatcgaagg cgccgaaagc
      721 cgggaatgga cgctgcaacg ccaggacggc agccgcttca tcgccagcgt tgccatcacg
      781 gcaatgcgcg acaacgatgg ccagctgacc ggctttctcg gcatagccat cgatatcacc
      841 cgccagaaaa acaccgaacg ggagctggcc agcagcctgg ccaccacgca ggccattctg
      901 gataccgcgc ccaacccgat catcaccgtc gacgacaaag gtcaggtgct atcgttcaac
      961 cccgccgggg aacgggtttt cggatatggc cagaacgaga tcgtgggaca gaacgtgcga
     1021 ctgctgatgc ccgactcgaa ccgtccggac cacgcaaacc tgtttgcaca ttaccgtcgc
     1081 gacgacaact ccgccgtggt tgataccggc cgcgaagtgc tggcccgtca caaggacggc
     1141 accgtcttcc cgattcatct tgctgtcggc acgatgacct tcgccggcac gcgccggtac
     1201 gttggcctca ttacggatct caccgaacaa tatcagcaga gaatggcatt gctggcggcg
     1261 cgggaccagc tgatgatggc ggcgaacgtt gccaggctcg gcatctggac ctggacactg
     1321 gcggacaatt ccctgcagtg gaacgaccgg atgttcgaac tgtatgacta tccgctgtca
     1381 ttgcgtgaaa ccggactgaa ctactcgcac tggcgccagc gcctgcatcc cgacgacagg
     1441 caggcgaatg accagagtct gtcggccgcc ctggaaggcc ggggtaatta cgacatcatt
     1501 ttccgcatca tcctgcccga tggccgggtc cgttttctgc aggtagccgc ctacatcgaa
     1561 cgtgacgaac gcaaccagcc ggtgaaagtc accggtatca acctggacat cacggcgcag
     1621 cgcgaactgg aaacccgcct gctgcaagcc aaggagcagg ctgatctggc cagcgctgcc
     1681 aagtcctcct tccttgccaa catgagccac gaaatccgca cgccgatgaa cgccgtgctc
     1741 ggcctgttac aactggtgcg ccagaccgaa ctgaatctgc gccagcagga ctacgtcgac
     1801 aaggccctga gttccggccg cgccctgctc agcctgctca acgatatcct cgactattcc
     1861 aagatcgagg cgggcaaact tgaactggac ctgcatccac tcgaactgga tgcgttaatg
     1921 cgagaactgg gcgatgtgct atccggcaac cagggcaaga aggatattga aatcctgttc
     1981 gcaatcgacc cgaatctgcc atcagtactg gtgggcgaca aactccgtct gttgcaggtc
     2041 atgatcaatc tcgccggcaa tgcgttcaag tttacccagc aggggcagat cgtcatcgga
     2101 gtggaagaac tgcaacggtc gaacggctcg atcctgctgc ggttctcggt gaccgatacc
     2161 ggcatcggca tcagcacaga acagctgcag cgcattttcc agagtttcac ccaggccgaa
     2221 gcctccactg cccgccgctt cggtggcagc ggtctgggcc tggtcatcag caagcgcctg
     2281 atcgagctca tgcacggcac actggaagtt accagtgaac cgggaaaagg cagccgattc
     2341 tggtttgacg tgcccctggg tgtcgcaaat ttcacaccca tgcgggaaag ctgtcccact
     2401 gccgacgtgc ccctgcacgt actgatcgtg gatgacaacc cggtcgctgc cgaattgatg
     2461 acggccactg cacgggcgct gggctggcgc acacagactg cgttcagcgg caacgatgca
     2521 ctggcccgcg tccgggacca ggccagccac aacgaccggt tcgacctggt gttgatggac
     2581 tggcgcatgc cggacatgga tggactcgac gccgcgaaac agatccgcaa cagcagcacg
     2641 acaccgggtt cgccattggt aattatggtc agcgcctacg aacgggaagc gatggcctcc
     2701 gagcaacagc atgccgatgc accctttgcg gatttcctca ccaaaccggt gacaccgcga
     2761 cagctggcaa acacggtaat gcaggcactg accgggacat ccgcaacccc ggccgccggc
     2821 gtggaagaca aaccccgcac ccagcgtttg cgcgacttgc gattactggt ggtggatgac
     2881 aatgcggtca accgtcaggt ggttgccgaa ttgctgcagg gtgaaggcgc cagcgtgaca
     2941 ctggcagaag gtggcgccga gggcgtggcc gccgtccacg ccaacccgga tacctttgac
     3001 gccgtcgtca tggacatgca gatgccggat gtagatggca ttgaagccac gcgccgcatc
     3061 cgcgccgacg cgcgcttctc ccggctgccc atcatggcga tgaccgccaa tgcctccacc
     3121 gcagaccggg atgcctgtct cgcggctggc atgaacgcac acgtgggcaa acccatcgat
     3181 ctggaacaga cagtcgccac attgcagacg ctgacgggcc gccgcacgct ccagccagtt
     3241 accttgcccc caccgcccat cgacacgcag gggcccctgg tggaatccct ggcggccatc
     3301 atgtcccgcc tcggtggcaa gcgtgcgctg tatgcctgca tgctgactct cttcgagccg
     3361 gaagaaaaca gcctgatcga tgaactcgcg gcgaagatca cacaccagga cattgccggc
     3421 agcgccgcca ccctgcatgc catcaagggc agtgccggca ccctgggcgc cacggctctc
     3481 gcgcagcacg ccgctgcact ggaattccgg ctgaaatcag gtgacgcgac cgcggttgaa
     3541 gcggtgatgt cgccagcgac agtgcagacg ctgcgacacc tgctgacaga atcggtccgc
     3601 gcgctgaacg cagaactggc cggggatacc gcccacccca caacgactgc cgtacagtct
     3661 gcccccagtc cgcgcgcaaa aatcctgata gtggacgatc aaccgctcaa catcagtatc
     3721 ctgaatgaat tgttccgcga tgaatatgaa ctgcactccg ccaccagcgg tgaacaggcg
     3781 ctggccttgt gcgccagcca gcagccggat ctggtgctgc tggatgtgat gatgccgggc
     3841 atggatggac acgatgtgtg ccgccagttg aaggctgatc cgctgaccag cacaatcccc
     3901 atcattttca tcaccggaca ggtcgatgag gcggaagaga tcatggcgct cgaactggga
     3961 gccgtggact tcatcaccaa acccatcaac gcggtggtgg tgatggctcg ggtgaaaacc
     4021 cacatcgccc tgaaacgaca gggcgatctg ctgcggtcaa atgcattgct ggatagtctc
     4081 acccgcattg gcaaccggcg caggttcgac gccgacctgc aaatattctg gcgccagtgc
     4141 ttccgggaaa gaacaccgct gtcactgatc ctgatcgacg tggatttttt caagaaatac
     4201 aacgaccgct acggccatgt attgggagat gcctgcctgg tgtccgtggc gcaggcactg
     4261 gagcattgct gccaacgtcc cttcgactcg ctggcacgct acggcggcga ggaattcgcc
     4321 tgcctgctgc ccatgacgga cctggccggg gccaccgcca ttgcacaggc aatgcagaca
     4381 caggtgcgca atctgaatat cgaacatctc gattcgacaa cagcgtccgt ggttaccatc
     4441 agcctgggtg tcgcgacact gataccggcc agcgacattt cacctgaaca gttgaccgaa
     4501 ctggcggacc aacggcttta cgcggctaaa acggcaggac gcggacaggt ctgtgccgcc
     4561 gcgccctgag agcactcacc cgccccgcta cgctgcgaga aatccattga ttgcctcagc
     4621 caccggcgca aacgtctctg cttccagatg caaatggtga ttaccgtcca acagcacctg
     4681 ctggcaaccc ggaatcttat cggcacggtt ctgcagggtt ttttccgtgg caaaaaaact
     4741 gtgacgaccg cgcaccagca acaccggcat ggtgagcttc tgcaaatagg cggtcaccat
     4801 atcttccgtc agccggatcg ccgacgacat tttcagacgc ggatcggatc gccaggtaaa
     4861 accctctccc gccggcatca gaccgcgatt gcagatgtgg gtggaggcat caacactgat
     4921 caccccgatg gcatgcgcgc gggtatgaat cgcatcgtca cgggtttcat acaccggctt
     4981 gcgcttggcg ctggtctttt tcatgtcctc cactgccttg cgcaacaccc tgggcgcatc
     5041 ttccgccgga ttggtattgc tgccgatgcc ctcgatcaaa atcagcttgg ttacgcgctc
     5101 gggaaaactg ccggcggtga aggtggcgat gccggcgccc atcgaatggc ccatcagaat
     5161 gaattcctgc aatcccagcg catccgccag accgatcaca tcgtccacgt tgtcaattgt
     5221 gtggtaacgc acaccctcgg gccggtgacc gctgtaaccg tggccggcaa agtccagcgc
     5281 gatcacgcga tgttgcgtca gcgctggtga cagccggtcg tacgacgccg cattgtccag
     5341 ccagccgtgc aacgcaatga ttgccggcag ctccggatcg ccccactgct tcgccgccag
     5401 ctccattccg ttgacctgta cacggatttc ttcggtgatc atggctggca ctcccggata
     5461 aattgatgaa tgagttcagc cgccacatcc ggctgctgca aaggatacat gtgctcgcca
     5521 ggaatctgtt ccagcggaat actgaaatgc cgcgcgaacg gcaccaccat gtcggcgcgg
     5581 ctggcatcgc tgcggtcggc gtacaccagt ttcatcggca gctgcggcgg tcgcttgcag
     5641 gatttcaggt tgtctgacgt gttgcggaaa atctccacct ccacgtcaac gcggaagcgc
     5701 agtttcacct gatcgccttc gctctggtcg atggcagcat tgcagaacga ttcaaaacac
     5761 tccggatgga actggaacag gcgcttcgac gcaaaatatt cgatggcatt ggcgcggttg
     5821 ggccaggatt gtttgcgatt tttcgatttt ccggcgggcg tgaaatcatc ggacttgccc
     5881 agcagcttca gcaccttgaa caccatcgcc atgcgccccc acatcagcgg cgggtccagc
     5941 atcagcacgc cccggaacag ctccggacgc ttgcaggccg cgatgaacgt aaccacagcg
     6001 cccatcgaat gccccacacc cagcaccggt tcctgggcgt tgcgctgcag gaagtcgatc
     6061 agctcgtccg ccagatagcg ccagttgtgg gtacagggaa atgcgggatt gtggccgaac
     6121 atcggcagtg caatcaccgt gtgaggttgc agccgatcaa atatcggctg ataggccgcc
     6181 gcgggcacgc cgttggcgtg ggcgaaatga acaagcatga taacgtcttc cccatggtta
     6241 actggtcgta aagcctggca gatgctgatg ataaccgctc agggccttac tggctgcacc
     6301 cggtacccag ctttgcgcaa cagttgcagc aacccgtgct cccctggcag gtgcaatgca
     6361 cccaccgcaa ccaaattacc ggatcggttc aggtacacac gcagccgttc gaacatg
//
LOCUS       DASUEG010000463         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_826800, whole genome shotgun sequence.
ACCESSION   DASUEG010000463 DASUEG010000000
VERSION     DASUEG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826800"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFX11_08635"
     CDS             complement(<1..479)
                     /locus_tag="VFX11_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938726.1"
                     /translation="MNWFNRISIKFKLLLIPLVGVLGFATNLAYNFSVNTETRHKLES
                     VRDTYYPILERANTVMVTLDRTTEILNSAVSAGELDMMKGADENSEKMRSLFREIKSL
                     EPNRKREVEDLAASFENYYQLAREISEGMISGKADFAQLNSKVELMGSKLNSLKSQLR
                     "
     gene            complement(603..2021)
                     /gene="cysG"
                     /locus_tag="VFX11_08640"
     CDS             complement(603..2021)
                     /gene="cysG"
                     /locus_tag="VFX11_08640"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX4938727.1"
                     /translation="MEYLPVFYQLKNQRCLVVGGGEVATRKASMLQRAGANVRVVALE
                     FSAAMEQLLAKNTRSEKHQRTFSPKDLDGVALVIAATDDEALNREVSAQAKARYIPVN
                     VVDNQELCTFILPSIVDRSPLVIAVSSGGQSPVLARLIRAKLETLIPASYGRLAHLVG
                     QFRDQVKARFGDVNQRRGFWENVLSGPIAEYVFAGKERDAEQMLQQLLENPSMPAPHK
                     GEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVSPEILDLVRRDATRIYVGKARS
                     AHAVPQDQINEYLVELALKGHRVCRLKGGDPFIFGRGGEEIDRLAQAGIQFQVVPGIT
                     AASGCASYAGIPLTHRDHAQSVRFVTGHLKDGSLDLDWPTLAREKQTLVFYMGLVGLP
                     SICERLIAHGLAETTPIALIQQGTTRHQRVFVSDLANMVEHVKNQEVKAPTLIIVGDV
                     VKLHDKLKWFDPSATGEASSSF"
     gene            complement(2043..>2479)
                     /locus_tag="VFX11_08645"
     CDS             complement(2043..>2479)
                     /locus_tag="VFX11_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014108778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX4938728.1"
                     /translation="VELVQIAHPSRSYELLEELTGHAETILNRLDLPYRTVMLCTGDM
                     GFSSAKTYDIEVWLPGQGKYREISSCSNFEDFQARRMQARYRPKDGKKPELLHTLNGS
                     GLAVGRTLVAIMENYQQADGSIRVPDALKPYMGGVDVISVQA"
BASE COUNT          527 a          751 c          690 g          511 t
ORIGIN      
        1 cgcaactggg atttgaggga gttcagcttg gatcccatca gctcgacctt ggaattgagc
       61 tgggcaaaat cggccttgcc ggaaatcatg ccttctgaaa tctcccgcgc cagctggtag
      121 taattttcaa aactggcagc cagatcttct acctcgcgtt tgcgattcgg ttccagcgat
      181 ttgatctcgc ggaacagcga tcgcattttt tccgagtttt cgtccgcgcc tttcatcatg
      241 tccagctcgc cggcactgac tgcggaattg aggatttctg tggtgcggtc cagcgtcacc
      301 ataacggtgt tggcccgctc cagaatcgga tagtaggtgt cacgcacact ttccagcttg
      361 tgccgggttt cggtgttgac cgaaaagttg taggcaagat tggttgcgaa ccccagaaca
      421 cccaccaacg ggatcagtag caacttgaat ttgattgaga tacggttgaa ccagttcatg
      481 tagcaagctc cggcgaaaac tccctatacc aatttaaagg ttagacgatg ccggatgttt
      541 gaaaaggaat cggccctgaa aaacccgtgg ttatcaaggc cgaaggcggg aaatgcaggg
      601 aatcagaatg acgacgacgc ctcgccggtg gcggaaggat caaaccattt gagcttgtcg
      661 tgcagcttga ccacatcgcc aacgatgatc agggttggcg ctttaacttc ctgattttta
      721 acgtgttcaa ccatgtttgc cagatcggat acaaacaccc gctgatgccg ggtggtaccc
      781 tgctggatca gggcgatcgg cgtcgtttcc gccagtccat gggcgatcag acgttcgcag
      841 atggacggca gcccgacgag gcccatgtag aacaccaggg tctgcttttc acgggccagg
      901 gtgggccaat ccagatccag ggagccgtcc ttgagatgtc ccgtaacgaa ccgcaccgac
      961 tgggcatgat cgcgatgggt cagcggaatg ccggcgtaac tggcgcaacc agatgccgcc
     1021 gtaatgcccg gcaccacttg aaactggatg ccggcctggg ccagccggtc gatttcctcg
     1081 ccgccgcgtc caaagataaa ggggtcaccc cccttcaacc ggcaaacacg atggcctttc
     1141 agggcgagct ccacgaggta ttcgttgatc tggtcctgcg gcactgcatg agcactgcgg
     1201 gcctttccaa cgtatatacg cgtggcatcg cggcgcacca gatccagaat ctcggggctc
     1261 accagacgat cgtacagcac cacatccgcc tgctgcatca accgcagggc acgaaacgtc
     1321 agcagatcgg gatcaccggg gccggcaccc accagataca cctcgccctt gtgcggcgcg
     1381 ggcatcgatg gattttccag caactgttgc agcatctgct cggcgtcacg ctccttgccg
     1441 gcaaatacat attccgcgat cggtccggac agcacattct cccagaagcc acgccgctgg
     1501 ttcacatcgc caaaacgcgc tttgacctga tcgcggaact gccccaccag atgggcaaga
     1561 cgaccatagc tggccggaat gagcgtttcc agcttggcgc ggatcagccg tgccagcacc
     1621 ggcgattgac cgccgctgga aacggcgatc accagcggcg aacgatccac gattgaaggc
     1681 aggatgaagg tgcacagttc ctgattgtcc acaacattga ccggaatgta ccgggctttc
     1741 gcctgcgccg acacctcgcg gttcagggct tcgtcatcgg tagctgcgat gaccagcgca
     1801 acgccgtcca gatccttcgg ggaaaacgtg cgctggtgtt tttccgaccg ggtatttttc
     1861 gccagcaact gctccatcgc cgcggaaaac tccagcgcca ccacgcggac attggcgccg
     1921 gcacgctgca gcatggacgc cttgcgcgtc gccacttcgc caccacccac caccaggcag
     1981 cgctgattct tcaactgata aaaaaccggc agatattcca tcgttcaacc attcatcctg
     2041 attcaggcct gcacggaaat cacgtcgaca ccgcccatgt aaggcttcag cgcgtcgggc
     2101 acgcggatgc tgccatcggc ctgctggtaa ttttccatga tcgccaccag cgtccgcccg
     2161 accgccagcc ccgatccatt cagtgtatgc agcagttccg gcttcttgcc atccttcggc
     2221 cggtagcggg cctgcatgcg gcgggcctga aaatcctcga agttggagca ggacgaaatc
     2281 tcgcggtact tgccctgtcc cggcagccag acttcgatgt cgtaggtttt cgccgaggaa
     2341 aagcccatgt cgccggtgca cagcataacg gtgcggtacg gcaggtcgag cctgttcagt
     2401 attgtttcgg cgtggccggt cagttcctcc agcaactcgt atgatctgga gggatgcgcg
     2461 atctgcacca gctcgacct
//
LOCUS       DASUEG010000464         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1414816, whole genome shotgun sequence.
ACCESSION   DASUEG010000464 DASUEG010000000
VERSION     DASUEG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414816"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFX11_08650"
     CDS             complement(<1..792)
                     /locus_tag="VFX11_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEX4938729.1"
                     /translation="MNPSSFADIIAALDQGTVIPYLGPGVLRGVTDTVSGTPIPAESQ
                     DLILAMTGGQPMAPRLMYEFPRAAMHMENKKGRSFLERFLNTTYGERTWSASEFHQWL
                     AARRLPYVIDCNRDTQLQQLYADRDHTLVLGASRLTGTHYRFDLHEFRDGAYRHITQN
                     EVNPDLPVLFKPMGSPLPKPSYVASDADFVDYITELMGGFAVPAWLKQYRQQKRYLFL
                     GMRFNRDTERMVMSDLIYDAADHAGWAFIPEPNAKEARFCARKNIQ"
     gene            1230..2111
                     /gene="nifH"
                     /locus_tag="VFX11_08655"
     CDS             1230..2111
                     /gene="nifH"
                     /locus_tag="VFX11_08655"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HEX4938730.1"
                     /translation="MAMRQCAIYGKGGIGKSTTTQNLVAALAELGKKVMIVGCDPKAD
                     STRLILHSKAQNTIMEMAAEAGTVEDLELEDVLKTGYGGIKCVESGGPEPGVGCAGRG
                     VITAINFLEEEGAYEDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVVSGEMMAMY
                     AANNITKGIVKYANSGGVRLAGLICNSRNTDREDELIIALADKLGTQMIHFVPRDNVV
                     QRAEIRRMTVIEYDPKAKQADEYRSLARKIVDNKKLVIPTPVTMDELEALLMEFGIMD
                     EEDLSVVGKTAAEEAVA"
     gene            2264..>2849
                     /locus_tag="VFX11_08660"
     CDS             2264..>2849
                     /locus_tag="VFX11_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX4938731.1"
                     /translation="MSGMTREEVESLIQEVLEVYPEKAKKDRAKHLSPNDTSLEQSKK
                     CITSNKKSLPGVMTIRGCAYAGSKGVVWGPVKDMIHISHGPVGCGQYSRAGRRNYYIG
                     TTGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEIETLFPLNKGITIQSECPIGLIG
                     DDIEAVAKKKGAEHNKTVVPVRCEGFRGVSQSLGH"
BASE COUNT          677 a          766 c          842 g          564 t
ORIGIN      
        1 ctggatattt ttgcgggcac agaagcgggc ttccttggcg ttgggctcgg gaatgaaggc
       61 ccagccagcg tgatctgccg cgtcgtagat caggtcgctc atcaccatgc gttcagtgtc
      121 gcgattgaag cgcatgccca ggaacagata gcgcttctgc tgccggtact gcttgagcca
      181 ggccggaacg gcaaagccgc ccatcagttc ggtgatgtaa tcgacgaagt cggcgtcgga
      241 cgcaacatag ctcggcttcg gcagcgggct gcccatgggt ttgaacagca ctggcagatc
      301 cggattcacc tcattctggg tgatgtgccg ataggcgccg tcacggaatt catgcagatc
      361 gaaccggtaa tgagtgccgg taagacggga tgcacccagt accagggtgt gatcgcggtc
      421 cgcatacagt tgttgcagct gggtgtcacg attgcaatcg attacgtacg gcaaccggcg
      481 tgccgccagc cattgatgga attccgaggc cgaccaggta cgttcgccgt aggtggtatt
      541 caggaaccgt tccagaaaac tgcggccttt cttgttttcc atgtgcatgg cggcgcgcgg
      601 gaattcatac atcagacgcg gcgccatggg ctggccgccg gtcatggcca gaatcagatc
      661 ctggctttca gcgggaatgg gcgttccact cacggtgtcg gtgacgccac gcaaaacgcc
      721 ggggcccagg taaggaatca cggtgccctg gtcaagggcg gcaatgatgt cggcaaaaga
      781 tgaagggttc accggctgta tctcctgtag cggatttttt tatggacacg ctatgcagag
      841 caaaatgcaa gccaccggaa actcgccatc ccgtcacggc aactgattga aattaaacac
      901 acaatgcgat ccggccgctg cagcgtgcgt gatccggtca cgacaaattg tcgggatgcc
      961 gacaaaaaaa tccgctgccg gacgtgcagc aaaccgctct ggtcgtgttg tcgacattgc
     1021 aacaagccag catggggatt gtgggggttt gttggttatg tcacgcctgt gcagcctgtc
     1081 atcaatctga attatttctt tgtttacagc cgtttgcgct acaaaaaccg ttttggcaca
     1141 aagcctgctc cagtaccgct gcatagcgca ctcacgcttt acagcccacg acacaatcgt
     1201 ggaatgacaa cctggaggag accttcaaca tggcaatgcg tcaatgtgca atttacggaa
     1261 aaggcgggat cggcaaatcc accactactc agaatctggt ggcggcgctg gctgaactcg
     1321 gcaagaaagt gatgattgtc ggctgcgatc ccaaagcgga ttcaacccgt cttattctgc
     1381 actccaaagc gcagaacacc atcatggaaa tggcggcaga agctggcacc gtggaagacc
     1441 tggagctgga agacgtgttg aagaccggat acggcggcat caagtgcgtt gagtccggcg
     1501 gtcccgaacc tggcgtgggc tgcgccggtc gtggtgttat cactgcgatc aacttcctgg
     1561 aagaggaagg ggcttacgaa gacgatctgg acttcgtttt ctatgacgta ctgggcgacg
     1621 tggtgtgcgg cggtttcgcc atgccgatcc gcgaaaacaa ggcacaggaa atctacatcg
     1681 tggtgtccgg tgaaatgatg gccatgtacg cggccaacaa catcaccaag ggcatcgtga
     1741 agtacgccaa ctccggcggc gtgcgtctgg ctggcctgat ctgcaacagc cgcaacaccg
     1801 atcgcgaaga tgaactcatc attgccctgg ccgacaagct gggtacccag atgattcatt
     1861 tcgttccgcg cgacaacgtc gtacagcgcg ccgaaatccg tcgcatgacc gttatcgaat
     1921 acgatccgaa agccaaacag gccgatgaat accgcagcct ggcacgcaag atcgtggata
     1981 acaaaaagct ggttatcccc acaccggtaa ccatggacga gctggaagcg ctgctgatgg
     2041 aattcggcat catggacgaa gaagatctga gcgttgtagg taaaacggct gcagaagaag
     2101 cggtcgcttg atcctgaggt aagaagaatc caccggatgg caggaagcag gcagtcgtca
     2161 tccggcggct tctgcaggcc ggggcaggca gattggtcct gccggaagtg ctcgcaagag
     2221 tgcgaccgtt acccgcaaat gtacaaattc aggagacatg attatgtctg gcatgacacg
     2281 cgaagaggtg gaaagcctca ttcaggaagt tctggaagtc tatccggaaa aagccaagaa
     2341 agaccgggcg aagcacttgt cgcccaacga caccagcctt gagcaatcga agaagtgcat
     2401 cacatcgaac aagaaatccc tgcccggtgt gatgaccatc cgcggttgcg cctacgctgg
     2461 ttccaagggt gtggtgtggg gcccggtgaa ggacatgatc cacatttccc acggtccggt
     2521 gggctgtggt cagtactccc gcgccggccg tcgcaactat tacatcggca ccactggcgt
     2581 caacgccttc gtcaccatga actttacctc ggatttccag gagaaggaca tcgtgttcgg
     2641 cggcgacaag aagctggcca agctgatcga cgaaattgaa accctgttcc cgctcaacaa
     2701 gggcatcacc attcagtcgg aatgtcccat cggcctgatc ggcgacgaca ttgaagcggt
     2761 ggccaagaaa aaaggcgccg agcacaacaa gaccgtggta ccggtacgtt gcgaaggttt
     2821 ccgcggcgtg tcgcagtcgc tgggccacc
//
LOCUS       DASUEG010000465         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1975780, whole genome shotgun sequence.
ACCESSION   DASUEG010000465 DASUEG010000000
VERSION     DASUEG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975780"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFX11_08665"
     CDS             complement(<1..1298)
                     /locus_tag="VFX11_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4938732.1"
                     /translation="MARFFIDRPVFAIVLSLFITLSGLLALGVLPIAQYPQITLPTIN
                     VSTQYIGANADVVLESVAQVVEDKVNGVEGMLYMDSQSTGNGLYSLNVTFGLDKNADM
                     ASVLTQNRVTTATPSLPSEVNQVGVTLRKQTPDVLMYYALHSPGGTYDPLFLKNYGTI
                     YVTDVLKRIPGVGNVSEYGSDFGLRIWLDPTRMSKQGITVNDILQAVKEQNIQVPAGT
                     LGQYPAPPHQVFQYSVLVQGRLVAAEEFCAVILKSGGAGQFVRIRDVARCELGAKDYL
                     YFGSFNGKPAAIYAINLTPDANALDVAEKIRAEMEQLGRNFPPDLALDIVTDNTLFVT
                     ESMTEVLHTLFEAMALVLIVVTLFLQSWRATLIPMLAVPVSLIGTLALFLALGFTINT
                     LSLFALVLAIGIVVDDAIVVVEAVEYHMKVNGLSPRDATVR"
     gene            complement(1309..>2380)
                     /locus_tag="VFX11_08670"
     CDS             complement(1309..>2380)
                     /locus_tag="VFX11_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4938733.1"
                     /translation="PVATIPEVGVQNPQRRSIEVERDFIGEVKATEEAEIRSKVTGRV
                     VSVEFKEGTLVQLNQPLFRIDSESLKSAVDEAVAEVARARANLDQAKADAARYKSLVS
                     KGTISRQQYDDAVSKQAQAVATHAAANAKLDQARTQLRESAILSPYDGRIGRALVNVG
                     ALVTANQTLLATVSTTATARVDFALSEREYIRMMQDRLREEQTYEEIQVKLLLAEGSL
                     YPEIGRITFSDRAISADTGTFAVSATFPNPHEWLRPGMFARVRVRVATVPDAMLIPQR
                     AVQEILDKTFVSLVDANGKVERRAVVLGSNDGDEVVVKEGLQDQDQVIVEGHHKVRPG
                     APVKAVPLTPPVVETAATSANG"
BASE COUNT          461 a          780 c          681 g          458 t
ORIGIN      
        1 cgcacggtgg catcacgggg cgacagtcca ttcactttca tgtgatattc caccgcctcc
       61 accaccacga tcgcgtcgtc caccacgatg ccgatcgcca gcaccagcgc gaacaacgac
      121 agtgtgttga tggtaaagcc cagtgccaga aacagtgcca gcgtgccaat caggctcact
      181 ggcaccgcca gcatgggaat cagcgtggcg cgccagcttt gcaggaacag cgtgaccacg
      241 atcagcacca gtgccatggc ttcgaacagc gtgtgcagca cttcggtcat ggattccgtg
      301 acgaacagtg tgttgtcggt gacgatatcc agcgccagat ccggcggaaa attccgcccc
      361 agctgctcca tctccgcgcg gattttttcc gccacatcca gcgcgttggc atccggtgtc
      421 aggttgatgg catagatcgc ggcaggcttg ccattgaagc tgccaaaata cagataatcc
      481 ttcgccccca gttcgcagcg tgccacgtcg cgaatccgca caaactgtcc ggcgccgcca
      541 cttttcagga tcaccgcgca gaattcctcc gccgccacca gccggccctg caccagcacc
      601 gaatactgga acacctggtg gggcggcgcc ggatactggc ccagcgtacc ggcgggcacc
      661 tggatattct gttccttcac cgcctgcaga atatcgttca ccgtgatgcc ctgtttcgac
      721 atgcgggtag gatcgagcca gatgcgcaat ccgaaatcgc tgccgtattc gctgacgttg
      781 cccacaccgg gaatccgttt cagcacatcg gtaacgtaga tggtgccgta atttttcagg
      841 aacaggggat cataggtacc gcccggtgaa tgcagcgcgt aatacatcag cacgtccggc
      901 gtctgcttgc gcagtgtcac gcccacctgg ttcacttccg acggcaacga cggggtggcg
      961 gtagtcacgc ggttctgtgt cagcaccgac gccatgtccg cattcttgtc cagaccaaag
     1021 gtgacgttca gcgaatacag gccgttgccg gtggattgcg aatccatgta cagcatgcct
     1081 tccacgccgt tgaccttgtc ttccaccact tgcgccacac tttcgagcac cacgtcggca
     1141 ttggcgccga tgtactgggt cgagacattg atggtgggca acgtgatctg cggatactgg
     1201 gcaatgggca gcacacccag cgccagcagg ccgctgagcg tgatgaacag cgaaagcaca
     1261 atggcgaaca ccggccggtc gatgaagaaa cgcgccatga atccgccctc aaccgttcgc
     1321 agaggtggca gcggtttcga ccaccggcgg cgtcagcggc acggccttta ccggagcacc
     1381 gggccgcacc ttgtgatgac cttccacaat gacctgatcc tgatcctgca acccttcctt
     1441 caccaccact tcatcgccgt cattcgagcc cagcaccacc gcgcgccgct ccaccttgcc
     1501 attggcatcc accaggctga cgaaggtctt gtccagaatt tcctgcaccg cccgctgcgg
     1561 aatcagcatc gcatccggta ccgtggccac ccgcacccgc acccgggcga acatgccggg
     1621 ccgcagccat tcgtggggat tgggaaacgt cgcagacacg gcaaaggtgc cggtatcggc
     1681 ggaaatggcg cggtcggaaa aagtgatacg gccgatctcg gggtacagac tgccttcggc
     1741 cagcagcagt ttcacctgga tttcctcgta ggtctgctct tcccgcaaac gatcctgcat
     1801 catgcggatg tactcgcgct cgctgagggc gaaatccacc cgcgccgtgg cggtggtgga
     1861 gacggtggca agcagcgtct ggttggcagt gaccagcgca ccgacattga ccagtgcacg
     1921 gccgatgcgg ccgtcatagg gggaaagaat cgcggactcc cgcaactggg tccgggcctg
     1981 atccagcttg gcgttggccg ctgcatgggt tgcgacggcc tgcgcctgct tgctgacagc
     2041 gtcgtcgtac tgctggcgcg aaatggtgcc cttgctcacc aggctcttgt agcgggcggc
     2101 gtcagccttg gcctgatcca ggttggccct ggcccgcgcc acttccgcca cggcttcatc
     2161 caccgctgac ttcaggcttt cactgtcgat gcggaacagc ggctggttca actgcaccag
     2221 cgtgccttcc ttgaattcca ccgacacgac gcggccggtg accttggaac ggatttcggc
     2281 ttcctcggtg gccttcacct cgccgataaa atcgcgctcc acctcgatcg accgacgctg
     2341 cggattctgc acgcccactt cagggatggt cgccaccggt
//
LOCUS       DASUEG010000466         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1969432, whole genome shotgun sequence.
ACCESSION   DASUEG010000466 DASUEG010000000
VERSION     DASUEG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969432"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFX11_08675"
     CDS             complement(<1..226)
                     /locus_tag="VFX11_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938734.1"
                     /translation="MARQSPSAPTPSHLAARHLPLLLLLYLVTPVLTLHFVLIPHLLR
                     PSLPTDVPVTQAPLPEEPKPDFSSIDSVPER"
     gene            483..1724
                     /gene="serB"
                     /locus_tag="VFX11_08680"
     CDS             483..1724
                     /gene="serB"
                     /locus_tag="VFX11_08680"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX4938735.1"
                     /translation="MREILLINVSGPDKPGVTSTVTDLLSRSNVNVLDIGQAVIHDTL
                     TLGMVVEVPAEEDSSPILKDVLFACHGLGMSVRFIPIAEEEYEDWASGQGKDRYIVTL
                     LARKITARQIAAVTRIIAGHGLNIHAINRLSGRISLQREAPDQKACVELYVRGALKDI
                     ARMRAQFLEVAGELGVDIAFQADNAYRRNRRLVCFDMDSTLIEAEVIDELAKAAGVGE
                     QVAAITEQAMRGELDFTQSFKRRVAFLKGLDESVLEGIARNLPITEGAEDLVRALKAL
                     GYKTAILSGGFNYFGRFLQAKLGIDYVFANELAIENGKVTGEVVGRVVDGKRKAELLR
                     ELADKEGLNLQQVIAVGDGANDLPMLGLAGLGIAFRAKPLVKENADHSISTLGLDGIL
                     YLLGVRDQDSEELLKWYDERE"
     gene            complement(1752..>3329)
                     /locus_tag="VFX11_08685"
     CDS             complement(1752..>3329)
                     /locus_tag="VFX11_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX4938736.1"
                     /translation="LRTFLVTAGIFGAALFYGDGMITPAISVLSAVEGLKVVAPGLED
                     YVIPVTITVLVLLFAVQKSGTGSVGAIFGPVMIIWFGTLGILGILSIAQHPDVLAALN
                     PVHAVTFFPENGLASFLVFGAVVLAVTGGEALYADMGHFGRKPIRLAWLGLVLPALTL
                     NYFGQGALLLYEPGAIDNPFYHLAPDWFQWPLLALATCATVIASQAVISGVFSVTRQA
                     VQLGFWPRTNIKHTSNKEIGQIYIPQMNWFLLVAIILLVLGFRTSSNLAAAYGIAVTA
                     AMMVDTLLACFVANRLWGWNPLLVLGMGLCFFTLDFAFLGSNMLKLIDGGWFPLTVGL
                     VMLTLMLTWRRGRRVLFDKLEQETMPLEPFADSLMLSPPTKVPGTAVFMTSTPDRVPH
                     ALLHNLKHNKVMHERIVLLNVSTREIPLVSPHDRIDIREVRPGFYVIRAYYGFKESPD
                     VPQLLQDCAAFGLTFNMMDTSFFLSRERLIPSAHPAMPPILDRIFVLMTRNTMNATDF
                     FNIPTNRVVELGTQIEI"
BASE COUNT          687 a          982 c         1054 g          606 t
ORIGIN      
        1 tgcgttccgg cactgaatcg atgctggaaa agtcgggttt gggttcttcc ggcagcggtg
       61 cttgcgtaac tggaacgtcg gttggcaggg acggccgcag caggtggggg atcaggacga
      121 agtgcagggt cagtacgggt gtcaccagat acagcaacag cagcaacggc aaatgccggg
      181 cagccagatg cgaaggggtg ggtgctgacg gggactgtcg tgccatgcgc acggctcctg
      241 tttggcgtcc ggccagcatt acgccatgat cgacccgggt ttgcaaccag ccgggttttg
      301 gccgatcctt cgcccagcgt gtagaatacg taacccgttg aaccggcagc tcggaacggt
      361 aaaggggttg gcaagacacg cggtgcctgt gtcactgtgc gcccgacggc agcatcccgg
      421 ctgctgacgg ccctggtcca tcgcccttcc cgtttggcaa ccttagttat gaaggaactg
      481 gcgtgcgcga aatcctgttg atcaatgtct ccggccccga caagccgggt gtgacgtcca
      541 ctgtgaccga cctgctgtcc cgctccaatg tcaatgtgct ggacataggc caggcggtga
      601 ttcacgacac cctgaccctc ggcatggtgg tggaagtgcc ggcggaggaa gattcctccc
      661 ccatcctgaa agacgtcctg tttgcctgtc acggcctggg catgtcggtg cgtttcatcc
      721 ccatcgccga ggaagaatac gaggactggg ccagcggtca gggcaaggac cgctatatcg
      781 tcaccctgct ggcgcgcaag atcaccgccc gccagatcgc cgccgtgacc cgcatcattg
      841 ccggacatgg cctcaatatc cacgccatca accgcctgtc cgggcgcatt tccctgcagc
      901 gcgaagcacc ggatcagaaa gcctgcgtcg aactgtatgt gcgtggtgct ctcaaggaca
      961 tcgcccgcat gcgcgcgcag tttctggaag tcgccggcga gctgggcgtc gacatcgcct
     1021 tccaggccga caacgcttac cgccgcaacc gccgtctggt gtgcttcgac atggattcca
     1081 ccctgatcga ggcggaagtc atcgacgaac tggccaaggc cgccggtgtc ggcgagcagg
     1141 tggcggccat caccgagcag gccatgcgcg gcgaactgga tttcacccag agcttcaagc
     1201 gccgggtggc gtttctgaaa ggactggacg aatcggtgct ggagggtatc gcccgcaacc
     1261 tgccgatcac cgaaggcgcc gaggatctgg tgcgtgcact gaaagcactg ggctacaaga
     1321 cggcgatcct gtccggtggc ttcaattatt tcggccgctt tctgcaggcc aagctgggca
     1381 ttgactatgt gtttgccaac gagctggcga ttgaaaacgg caaggtcacc ggcgaagtcg
     1441 tggggcgtgt ggtggacggc aagcgcaagg cggaactgct gcgtgaactg gcggacaagg
     1501 aaggcctgaa cctgcagcag gtgattgcag tgggcgacgg tgccaacgat ctgcccatgc
     1561 tggggctggc ggggctgggc attgccttcc gcgccaagcc gctggtgaag gaaaacgccg
     1621 accactccat ttccacgctg ggtctggacg gcatcctgta cctgctgggc gtgcgcgatc
     1681 aggacagcga ggaattgctc aagtggtatg acgagcgcga gtgacctgca cctctgagcg
     1741 tccccgcgct gtcagatttc gatctgcgtg cccagctcca ccacccggtt ggtgggaatg
     1801 ttgaagaagt cggtggcgtt catggtattg cgcgtcatca gcacgaaaat ccggtccagt
     1861 atcggtggca tggcggggtg cgcactggga atcaggcgtt cacggctgag gaagaacgac
     1921 gtatccatca tattgaatgt cagcccgaac gccgcgcaat cctgcagcag ttgaggcaca
     1981 tcggggcttt ccttgaagcc atagtaggcc ctgatcacgt agaagccggg ccggacttcc
     2041 cggatgtcga tgcggtcatg cgggctcacc agcggaattt cccgcgtact gacgttgagc
     2101 agcacgatgc gttcgtgcat gaccttgttg tgtttgagat tgtgcagcag ggcatgggga
     2161 acgcggtctg gcgtgctggt catgaatacg gcggtgccgg gcactttggt gggcggactc
     2221 agcatcagac tgtcggcaaa cggttccagc ggcatggttt cctgttccag cttgtcgaac
     2281 agcacccggc gaccgcgacg ccaggtcagc atcagcgtga gcatgaccag tcccaccgtg
     2341 agagggaacc agccgccgtc gatcagcttc agcatgttgg agcccaggaa ggcaaaatcc
     2401 agcgtgaaga agcacaggcc catgcccagt accagcagcg ggttccagcc ccacagacgg
     2461 ttggccacaa agcaggccag cagggtgtcc accatcatgg cggccgttac cgcgatgccg
     2521 taagcggcgg ccaggttgga tgaggtgcgg aaacccagca ccagcaggat gatcgccacc
     2581 agcaggaacc agttcatctg cggaatatag atctgaccga tttccttgtt cgaggtgtgc
     2641 ttgatgttgg tgcggggcca gaagcccagc tgcacggcct gacgggtcac cgagaatacg
     2701 cccgaaatca cggcctgcga tgcgatcacg gtggcgcagg tggccagcgc cagcaggggc
     2761 cactgaaacc agtccggtgc cagatgatag aaggggttgt cgatggcacc tggctcatac
     2821 agcagcaggg cgccctgccc gaaatagttc agggtgagcg ccggcaatac cagcccgagc
     2881 cacgccagcc ggatcggttt gcggccgaaa tgacccatgt cggcgtacag tgcttcaccg
     2941 ccggtcacgg ccagcaccac cgcgccaaaa acaagaaacg atgccagtcc gttttccgga
     3001 aagaatgtga cggcatgaac gggattcagc gccgccaata catccgggtg ctgcgcaatg
     3061 ctgagtatgc ccagtattcc cagggtgccg aaccagataa tcatgaccgg gccaaaaatg
     3121 gcacctacgc tgcccgtgcc gcttttctgc accgcgaaca gcaacaccag taccgtgatg
     3181 gtcactggaa tcacatagtc ttccagcccc ggtgccacta ctttcaagcc ttcgaccgcc
     3241 gacagtactg aaatcgccgg cgtgatcatg ccgtcgccat aaaacagcgc cgcgccaaat
     3301 ataccggccg taaccagaaa agtgcgcag
//
LOCUS       DASUEG010000467         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1685368, whole genome shotgun sequence.
ACCESSION   DASUEG010000467 DASUEG010000000
VERSION     DASUEG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685368"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1149
                     /locus_tag="VFX11_08690"
     CDS             364..1149
                     /locus_tag="VFX11_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEX4938737.1"
                     /translation="MMSELTPIMEAAINFAYVGAFVFVVMGLYLSYRKGKPHPLLLLC
                     ISAISFSWIEAPYDWAVYAQFPPAIPRMPAWWPLNMTWGGLPAAVPPGYIAYFVLPAV
                     IGVALAQWLIAHLQWRAPLTLLLVGLAVGCLWAFVFNAIIGARLGVFYYGRVIEGLAL
                     WPGTKHQYPLYDALAMGVQMMVFTYILGRTDSTGRSLVEVWADSRSKSRLGSSLRSIA
                     AVIVIGHVVYLSVFAPHFVTKVLHLQTVGPTEQLFEGVPNQPL"
     gene            complement(1192..>2270)
                     /locus_tag="VFX11_08695"
     CDS             complement(1192..>2270)
                     /locus_tag="VFX11_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938738.1"
                     /translation="DGNDVLRLGYTGGTTGKPKALASVQRTGLATLSIMMAEWEWPSP
                     LRFLSCTPLSHAGAAMFLPTLLKGGTMLVLPAFDPVQVLQTIQDYRINCVMLVPTMIY
                     ALLDHPRFDEFDLSSLETVFYGASAISPARLKEAIDRIGPVFMQFYGQAEAPMAVTVL
                     RKHEHDVNNPQRLASCGRPVPWLHVALLDTNNEPVADGEPGEICVRGPLVMNGYRDNP
                     QLTAEALSGGWLHSGDVAIRDPGGFLRIIDRTKDMIVTGGFNVYPRELEDILAEHPAV
                     SQVAVIGVPHEKWGEAITALVVLRKGHTVDARELIDRIAARKGSYQAPKTVEFIDSIP
                     LTPVGKPDKKVLRERYRTRAATPP"
BASE COUNT          439 a          606 c          676 g          549 t
ORIGIN      
        1 catgccaggc gttgtaccct tcaattgtgc gcacgccttg atggagcacg gcggcgaagt
       61 ccagatcaac gaaaccccgg gtacgctaca tgggattgac cgcgtaaaag ggagtgccat
      121 cacccgggca gccgttgaaa cacgaattgg atttttggag cggatattcg catgatcctg
      181 cttcaagtca ctgccggtga tgcggtgtga acaggtcggt atattgcaga ggttggagga
      241 ttctattgtg ggtgcattga tcatgctggg aacgcttttt ggattgatcg ttctgatttt
      301 cgcgttggtg ctgaagtttg atcccgaggc tcgtggaccc gccgataaag acgggagatc
      361 gaaataatgt ctgaactgac accgatcatg gaagcagcca tcaattttgc ctatgtcggg
      421 gcttttgtgt ttgtggtcat gggactgtat ttgagctacc gcaagggcaa gccgcatccc
      481 cttctgctgt tatgcatatc cgccatttca ttttcctgga tcgaggctcc gtatgattgg
      541 gcggtgtatg cgcagttccc gccggcgatt ccgcgcatgc cagcctggtg gccgttgaac
      601 atgacgtggg gagggctgcc ggcggcggtt cctcccgggt acatcgctta ttttgtactt
      661 ccagcggtga tcggtgtcgc tctggcgcaa tggctgattg cacacctgca gtggcgcgca
      721 ccgttgacgt tgctgctggt cggccttgcg gtaggctgcc tgtgggcttt tgtgttcaat
      781 gcgatcatag gggcaagact aggcgtgttt tattatggcc gtgtcatcga aggacttgcg
      841 ctctggcctg gtacgaagca ccagtatcct ctttatgacg ctctcgcgat gggggtgcag
      901 atgatggtct tcacgtatat tcttgggcgt acggattcca cggggcgctc gctagttgaa
      961 gtgtgggcag attcgcgttc gaaaagccgg ctgggctcat cattgcgttc cattgcggcg
     1021 gtgatcgtga tcggtcatgt ggtctatctc tccgtttttg cgccgcactt tgtcaccaaa
     1081 gtcctgcatc tgcagaccgt cggaccgacc gagcaactgt ttgagggtgt gccgaatcaa
     1141 ccgctttgac aggagcggga tcattcccgc ttctggaatc gacgccgtgt tttaaggggg
     1201 tgttgcggcg cgtgtgcgat agcgctctcg cagcactttc ttgtctggtt ttccaaccgg
     1261 tgtcagtgga atggaatcga taaactcgac tgtcttgggc gcctggtagg aacccttgcg
     1321 ggcggcgatg cgatcaatca gttcccgcgc atccaccgtg tggcccttgc gcagtaccac
     1381 cagcgcggta atcgcttcac cccatttttc atgaggaact ccaatgacag cgacctgcga
     1441 caccgccgga tgctccgcca gaatatcctc cagttcgcgc ggataaacat tgaagccgcc
     1501 ggttacgatc atgtccttgg tgcgatcgat aatccgcaga aagccaccgg ggtcgcggat
     1561 ggcgacgtcg ccgctgtgca accagccgcc ggaaagcgct tccgcagtca gttgggggtt
     1621 gtcccgataa ccgttcatta ccagcggtcc acgcacgcag atttcgccgg gctcgccatc
     1681 ggctactggt tcgttgttgg tatccagcaa tgccacatgc agccagggca ccggccggcc
     1741 acaactggcc agtcgttgcg gattattcac atcgtgttcg tgtttgcgca aaacggtcac
     1801 tgccatgggg gcttcagcct gaccgtaaaa ctgcatgaaa acggggccaa tccgatcgat
     1861 ggcctctttc agtctggcgg gggagatggc cgaggcgcca taaaaaacgg tttccaggct
     1921 ggataaatca aactcgtcga agcgcgggtg atccagcaat gcataaatca tcgttggcac
     1981 cagcatgaca cagttgatcc ggtaatcctg gattgtctgc aacacctgaa caggatcaaa
     2041 agccggcagc accagcatcg ttccaccttt cagcaatgtc ggcaggaaca tggccgcacc
     2101 ggcgtgactg aggggcgtgc aggataaaaa ccgcagcggg ctgggccatt cccattccgc
     2161 catcatgatg gaaagcgtcg ctagtccggt gcgctgtacg ctcgccagtg ctttgggttt
     2221 tccggttgtg ccaccggtgt agccgagtcg cagtacatcg ttgccatcta
//
LOCUS       DASUEG010000468         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_384933, whole genome shotgun sequence.
ACCESSION   DASUEG010000468 DASUEG010000000
VERSION     DASUEG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384933"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFX11_08700"
     CDS             complement(<1..938)
                     /locus_tag="VFX11_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX4938739.1"
                     /translation="MIYRKADHIILWVLVTPLLSYTAWTKLAVVYTHTPLTGWALLDF
                     LRLHGGYYGLILLWCTFLLRSSQRPLPWRLAVLALATYFIAADFAYSVTILHTGALAP
                     FDVAYYMGKAVNALQPPIQLMEWFTLALLPALFVALAALKWRQLHSGGAAPSLRFSGV
                     LVPLLLTALLVPPLHPQLELNTARPSYAYQIIEFLRQRTMLAEAHAEELPPALPLSLQ
                     TAPTQPRNLVIIALESVGAQATGLNNPALQDVTPFLNELARTSWVAQQAYTVTPHTSK
                     ALVAINCGRQPYPRHPIFESTYGLDTPCLAHLLREKG"
     gene            complement(1132..1542)
                     /locus_tag="VFX11_08705"
     CDS             complement(1132..1542)
                     /locus_tag="VFX11_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4938740.1"
                     /translation="MTITSLNPWQVFDQLQQDSLRRYVANGQRWQPSVDISESKERYE
                     IQLDLPGVKPEQVQVELEDGKLKISGEKKRLEAEGLAYRYKERTTGSFSRAFKLPEDA
                     DLNGIRAQFDNGVLVLTIQKQEAAKPRKIEIQVS"
     gene            complement(1703..2185)
                     /locus_tag="VFX11_08710"
     CDS             complement(1703..2185)
                     /locus_tag="VFX11_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HEX4938741.1"
                     /translation="MDLLTIAVFLAGCGAGAVVSYVVLARTRAESKIERQMRELQEEF
                     TAYRENVNQHFNKTAQLVNSLTENYIAVQKHLEDAADSFAEPPKSFALEESAKLPAAK
                     TEFVSLESRQQQELRSGKMDAEFGQLPVEPPKDYAPKTKPGDKGTLAEDFGLKDTKVY
                     "
     gene            2327..2983
                     /locus_tag="VFX11_08715"
     CDS             2327..2983
                     /locus_tag="VFX11_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4938742.1"
                     /translation="MNPFPADEQRIDLQGPAGVLKAIAAAPKNADNGPGNVTIVCHPH
                     SLMGGTMDNKVVHTVARARRDCGHRTVRFNFRGVAGSAGAFDHGIGESDDLLAVIDWA
                     RQVRPDDRIWLAGFSFGSFVAARTCARAVERGDPIDHLLLIAPAVVNYDFGPLRNFPV
                     PLQLIYGDADEVVDAQEIRRWSDGVQSRKSVHCLPDAGHFFHGRLTELKEILQQTASA
                     "
     gene            3389..>4453
                     /locus_tag="VFX11_08720"
     CDS             3389..>4453
                     /locus_tag="VFX11_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4938743.1"
                     /translation="MSIIRKGKPWRLNVGACGLGLLLAPALSAFAADPAATHKAETGA
                     DLAKSVAAGKVVYDKYCTGCHQPTGAGLTGAFPPLAKSDYLLADKQRAIGVVVKGLTG
                     EITVNGQKYNGAMPNFAYLKDQEIADVLTYALNSWGNNGGGVAAEAVAAARGSEATHA
                     EGPHPSTSEAEMRYAGAPAGIDPKSTKNMVTTEGPAMTTEEFDHAKTIFFQRCAGCHG
                     VLRKGATGKPLTTDITRAKGTDYLKALITYGSPAGMPNWGTSGDLTDKEIDVMARYLQ
                     HEPPQPPEFGMKEMKDSWKVTVAPDKRPKKKENNYNIENIFAVTLRDSGEVALIDGDT
                     KEIINVVKTGYAVHISRTSAS"
BASE COUNT          907 a         1256 c         1369 g          921 t
ORIGIN      
        1 cctttctcgc gcagcaggtg cgccagacag ggcgtgtcca gcccgtaggt ggattcaaaa
       61 atcgggtgac gcggataggg ctgccggccg cagttgatgg ccaccaacgc tttcgatgtg
      121 tgcggcgtca ccgtgtacgc ctgctgcgcg acccagctgg tgcgggccag ctcgttcaga
      181 aacggtgtga cgtcctgcag tgccggattg ttcaggccgg tggcctgcgc gcccaccgat
      241 tccagcgcga tgattaccag attgcgcggc tgtgtcggtg cagtctgcag actcagcgga
      301 agcgcgggcg gcagctcttc ggcgtgggct tccgccagca tggtgcgctg acgcagaaat
      361 tcgatgatct gataggcgta agaggggcga gccgtgttca gttccagttg cgggtgcagt
      421 ggtggcacca gcagggctgt cagcagcaac gggaccagca cgccgctgaa acgcagcgac
      481 ggcgcggccc cgccagaatg cagctgccgc catttcagtg ccgccagtgc aacgaacagg
      541 gccggcagca atgccagcgt gaaccattcc atcagctgga tcggcggctg cagtgcgttc
      601 acggccttgc ccatgtaata ggccacgtca aacggtgcca gagcgccggt gtgcaggatg
      661 gtgacgctgt aggcgaaatc cgctgcgata aagtaggttg ccagggccag cactgccagg
      721 cgccagggca atggcctttg gctggaacgc agcagaaagg tacaccagag caggatcagg
      781 ccgtaatagc ccccgtgcag ccgcagaaaa tccagcaggg cccagcccgt caacggtgtg
      841 tgcgtgtaga caacggcaag tttggtccag gcggtgtaag acagaagtgg agtaaccaat
      901 acccacagaa tgatgtggtc tgctttgcgg taaatcatgg tggcctgccc tggcgagtga
      961 aaaaaacctg atattccaat caggtctgaa aacgtaaatc cttctcagac gtggatatta
     1021 cagaaatatg acggcgtggt gaacgtccag gagccgaagg gcgtttggca gatccggact
     1081 ggcacgcggg gcgttccagt ccggatcaga taaggggcgt gcgggtttgt ttcagcttac
     1141 ctggatttcg attttgcgcg gtttggcggc ttcctgcttc tggatggtca gtaccagcac
     1201 gccattgtcg aactgggcgc ggatgccatt caggtcggcg tcttccggca acttgaatgc
     1261 ccggctgaag ctgccggtgg tgcgttcctt gtagcggtag gccaggcctt ctgcctccag
     1321 acgttttttc tcgccgctga ttttcagctt gccgtcctcc agttccacct gcacctgctc
     1381 cggcttcaca cctggcaaat ccagctggat ttcataacgt tccttgcttt cggaaatatc
     1441 cactgacggt tgccagcgct ggccgttggc cacgtagcgg cgcaggctgt cctgctgcag
     1501 ttggtcaaaa acttgccagg gattcaggct ggtgatggtc atggttgtgc tcctgttcaa
     1561 tgtgatctgg gggtttcgaa tcagtgacag gagtaaaaat ggggtgggaa caacaggctt
     1621 caaggggagg aaaaatatag tgtccgccgt attgaaaaag gggccgtagc ccctatttcc
     1681 gaacgcttgt gccaatcgtg tgtcagtaga ccttggtgtc cttcaggccg aaatcctctg
     1741 ccagggtgcc cttgtcgccg ggcttggtct tgggcgcgta gtccttgggc ggttccaccg
     1801 gtaactggcc aaattccgca tccatcttgc cggagcgcag ctcctgctgc tgacgacttt
     1861 ccagcgagac gaattcggtt ttggcggcag gcagtttggc cgattcctcc agtgcgaagc
     1921 tcttcggtgg ctctgcgaac gagtcggcgg cgtcttccag gtgcttctgc acggcgatgt
     1981 agttttcggt caggctgttg accagctgcg cagtcttgtt gaagtgctgg ttgacgtttt
     2041 cccggtaggc ggtgaactct tcctgcagtt cccgcatctg gcgttcgatc ttggattcgg
     2101 cacgcgtgcg cgccagcacc acataactca cgacggcgcc ggctccgcat cccgccagga
     2161 aaaccgcgat ggtcagtaga tccataaatt ctctccgcat cgaaataaac gactgatttc
     2221 acgatagtac aatgatccgg aagcggatga cagatggggg tgcgccgaat aacatgacga
     2281 cagcccgcaa cctgtaacaa gtcacttccg ccacgaggtt ttccccatga atccatttcc
     2341 cgcagacgaa cagcgcatcg acctgcaagg acctgccggg gtgctgaagg ccattgcagc
     2401 ggcacccaaa aacgctgata acgggcccgg aaatgtcacg atcgtctgcc atccgcattc
     2461 ccttatgggc ggcaccatgg ataacaaggt ggtgcacacg gtggcacggg ctcggcggga
     2521 ctgcggccac cgcaccgtcc gtttcaattt tcgcggggtg gccggcagtg ccggcgcgtt
     2581 cgatcacggc atcggtgaaa gcgacgacct gctggcggtg atcgactggg cgcggcaggt
     2641 gcgccctgac gaccggatct ggctggcagg tttttccttc ggctctttcg tggcagcgcg
     2701 cacctgtgcc agggcggtgg agcggggtga tcccatcgac catctgctgc tgatcgctcc
     2761 tgccgtggtg aattacgatt tcggcccgct acggaacttc ccggtgccgt tgcagctgat
     2821 ttatggcgat gccgacgagg tggtggatgc gcaggaaatc cgccgctggt ccgacggggt
     2881 gcagtcgcgc aagtcggtgc actgtctgcc ggatgcgggt catttctttc atggccggct
     2941 gactgaattg aaggaaatcc tgcagcagac agcgtcggcc tgaagaccct ccttgcctta
     3001 gaccggatat gtacgcgccc cctacctctt tcgtggtagg caagtgcacg gtagagaagt
     3061 tcatccttat tgtttgatcc cgcgcaagat tgtgtgtgtg ccgcaacaca taatgcaacc
     3121 aggtttattg cgaggaggag atcgcaaatg gatacactgc ttccggccgg cttcattctg
     3181 ggcatccttt cactgtcctg gctgctgttt cagggcgcct gcctttacct gctggttcta
     3241 ttcaatcccg aattatcctc cgcacacctc aacacaacct tgcctccgcc tgccgacggg
     3301 cggaacgcgc ggcagtaatc acctggtttc atcacttaca aaaaacatcc gccacgggcg
     3361 agctgtggcg cataacggga ggtagatcat gtctatcatc aggaagggaa aaccctggcg
     3421 actgaacgtg ggcgcatgtg gtttgggatt gctgctggct ccggcgttgt cagcttttgc
     3481 cgcagatcct gctgcgacgc acaaggcgga aacgggtgcc gatctggcga agtcggtggc
     3541 ggctggcaag gtggtctatg acaagtattg cacaggttgc caccagccta ccggcgcagg
     3601 tttgacaggg gcattcccgc cgctggcgaa atccgattat ctgctggcgg acaagcagcg
     3661 cgccattggg gtcgtggtga aaggtctcac cggtgaaatc accgtgaacg gccagaaata
     3721 caatggtgcc atgccgaatt ttgcctacct gaaagaccag gaaatcgccg acgtattgac
     3781 ctacgcgctg aattcctggg gcaataatgg cggtggtgtc gcggctgaag cggtggccgc
     3841 tgcccgcggt agtgaagcta cccatgccga aggcccgcac cccagcacat cggaagcgga
     3901 aatgcgctat gccggcgcgc cagcgggcat cgatccgaag tcaaccaaga acatggtgac
     3961 gaccgaaggc cccgccatga ccacggagga attcgatcac gccaagacga tcttcttcca
     4021 gcgttgtgcc ggttgccacg gtgtgctgcg caagggtgcc accggaaaac cgctcaccac
     4081 cgatatcacc cgcgccaagg gtaccgatta cctgaaagca ctgattacct acggttcgcc
     4141 ggccggcatg cccaactggg gtaccagtgg cgatctgacc gacaaggaaa tcgatgtgat
     4201 ggcgcgctac ctgcagcatg agccgccgca gccgccggaa ttcggcatga aggaaatgaa
     4261 agacagctgg aaggtgacag tcgcgcctga caagcggccg aagaaaaagg aaaacaacta
     4321 caacatcgag aacatctttg ccgtcaccct gcgtgattcc ggcgaagtgg cgttgatcga
     4381 tggcgacacc aaggaaatca tcaacgtggt gaaaaccggc tatgcggtgc atatttcgcg
     4441 cacctcggcg tcc
//
LOCUS       DASUEG010000469         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2121836, whole genome shotgun sequence.
ACCESSION   DASUEG010000469 DASUEG010000000
VERSION     DASUEG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121836"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..506
                     /gene="secB"
                     /locus_tag="VFX11_08725"
     CDS             24..506
                     /gene="secB"
                     /locus_tag="VFX11_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX4938744.1"
                     /translation="MTDQANSQQPQQQFQLQRIYLKDLSYEAPKTPQLFREQWKPDVN
                     LEINNNATVVEGNIWEVVLKITVTVKNEDAIAFIAEVSQAGLFLLQGIDGLQKEAILK
                     GVCPNILFPYVRETVTDVVSRGTFPQFLLQPINFEAAFAENLRQQQLKAQAEAEAAKH
                     "
     gene            557..1474
                     /gene="yedA"
                     /locus_tag="VFX11_08730"
     CDS             557..1474
                     /gene="yedA"
                     /locus_tag="VFX11_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEX4938745.1"
                     /translation="MKLRLPVVLALLGVYLIWGSTYLAMRFAIESIPPLLMAGSRYVA
                     AGVLLFSFLKWRGHAWPTLRQWRGSAIVGVLLLLGGNGMVGLALEQGVSSGMSALVVG
                     VTPLFALVFARFWGQRASGREWLGIGIGLFGLVLLNFGRELSTSPLGGVLLVLAALSW
                     SFGSIWGKHLDLPSSFMASALQMIVGGVALVLVGLARGEALTDMPSDKALWSLAYLVF
                     FGAILGFSAYAYLLETVRPALATSYAYVNPLVAVALGVWLGGESVDALELVAMAVILA
                     GVVMVYLPVARTPSPHLQPPAPATASQDR"
     gene            complement(1471..1935)
                     /gene="trmL"
                     /locus_tag="VFX11_08735"
     CDS             complement(1471..1935)
                     /gene="trmL"
                     /locus_tag="VFX11_08735"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX4938746.1"
                     /translation="MLHIVLYQPEIPPNTGNIIRLCANTGMQLHLIRPLGFALEDKQL
                     RRAGLDYHEWARVQVQDDFEDFLQKVNPARCFAMSTKGSVPYHEVRYQAGDAILMGPE
                     SRGLPLEIREHARVTGCIRIPMQPDNRSLNLSNATAIVAYEAWRQLGFEGAV"
     gene            2075..3031
                     /locus_tag="VFX11_08740"
     CDS             2075..3031
                     /locus_tag="VFX11_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938747.1"
                     /translation="MSSWLKRLITKKKPQSGAVLGVSLTPRGAGWCALDMAGATPVLT
                     GSAAINGRFRTPLEAFDALCQQQAPPALPCHLSLSHEFYNLLLVDAPNVPDAELREAV
                     KWRVKDLIAQPIEKMVVDVFRLPADAYRGRMNMLYAALIEKASVSPLVVACEREHLRL
                     EGIGVAELALAAVTRQLSGVENLSLALLHMTGDTGSINLVENGYLYLTRTLEMNAGGG
                     YSGNADFQPDPTDNLALDIQRSLDYYESQLGKSGVNRLYLVTESEQQQLWCESLSQRL
                     PIRAHLFPLADLFGMDPSLDPRQQGLLVPAVGAALGGRHGGT"
     gene            3018..>3401
                     /locus_tag="VFX11_08745"
     CDS             3018..>3401
                     /locus_tag="VFX11_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938748.1"
                     /translation="MAALEQRINFYQDIFHKPEIRYSFRQLTLILLVVMALLVALTTL
                     DYVRTEMQRRHVAQLQATQVRLEKAVVAMTEQLGRMVADPALEKQEVYLRESLQAKYQ
                     FLAQLRGQSDSHQVHFSDVLAGLSAL"
BASE COUNT          616 a          939 c         1112 g          734 t
ORIGIN      
        1 agaaaggtaa tcaggggaag agaatgaccg accaagccaa cagccagcaa ccgcagcagc
       61 agttccagtt gcaacgcatt tatcttaaag atctgtccta cgaggcgccg aaaacgccgc
      121 agctgttccg cgagcagtgg aagccggatg tgaatctgga aatcaacaac aacgcgacgg
      181 tggtggaagg caatatctgg gaagtggtgc tgaagatcac ggtgaccgtg aagaacgaag
      241 acgccatcgc gtttatcgcc gaggtttccc aggccggtct gttcctgctg cagggtattg
      301 acggtttgca gaaggaagcc atcctgaaag gcgtctgccc caacattctg ttcccctatg
      361 tgcgcgaaac cgtgactgac gtggtgtccc gtggcacgtt cccgcaattc ctgctgcagc
      421 cgatcaactt cgaagctgcc ttcgccgaaa atctgcgcca gcagcaactg aaagcgcagg
      481 ccgaagcgga agccgccaag cactgagttc ccgccttgtc gccgctgcgc gctgacacct
      541 ctgtaaggat gaatggatga agcttcgtct tcctgtggtg ttggcgctgc tcggcgtgta
      601 tttgatctgg ggttccacct acctcgcgat gcgctttgcc atcgagtcga ttccgccgtt
      661 gctgatggcg ggcagccgct atgtcgctgc gggtgtgttg ctgttttcat tcctgaaatg
      721 gcgcggtcat gcctggccga ccctgcggca gtggcgcggt tccgccattg tcggtgtgct
      781 gttgttgctg ggtggcaatg gcatggtggg gctggcgctg gagcagggtg tcagctccgg
      841 catgtcggca ctggtggtgg gtgtgacgcc gctgtttgcg ctggtgtttg cacggttctg
      901 ggggcagcgg gccagcggcc gggaatggct gggtatcgga atcggcctgt tcggcctggt
      961 gctgctgaac ttcgggcgcg agctgtcgac cagtccgctg ggcggtgtgc tgctggtgct
     1021 ggcggcgctg tcctggtcgt ttggctccat ctggggcaag catctcgatc tgccgtccag
     1081 tttcatggcc agtgccctgc agatgattgt gggtggtgtt gcgctggtgc tggtgggatt
     1141 ggcgcggggc gaggccctga cggacatgcc atcggacaaa gcgctgtggt cgctggccta
     1201 tctggttttc ttcggtgcca ttcttggatt ttcggcgtac gcctatctgc tggaaacggt
     1261 gcgccctgca ctggccacca gctacgccta tgtcaatccg ctggtggcgg tggcgctggg
     1321 cgtgtggctg ggtggcgagt cggtggatgc gctggagctg gtggcgatgg cggtgattct
     1381 ggcgggggtg gtgatggtgt acctgccggt ggcgcggacg ccatcaccgc acctgcagcc
     1441 gcccgcgccc gccaccgcat cgcaggatcg ctagaccgct ccttcaaatc ccagttgtct
     1501 ccaggcctcg tacgccacga tcgccgtcgc gtttgacagg ttcaggctgc gattgtccgg
     1561 ttgcatcgga atgcggatgc agccggtgac ccgcgcgtgt tcgcggattt ccagcggcag
     1621 gccccggctt tccggcccca tcaggatggc gtcacccgcc tgataccgca cttcgtgata
     1681 cggcacgctg cccttggtgg acatggcaaa acaccgagcc ggattcacct tctgcaaaaa
     1741 atcctcaaag tcatcctgca cttgcacccg cgcccactcg tggtaatcca gccccgcgcg
     1801 gcgcagttgc ttgtcttcca gtgcaaatcc cagtggccgg atcaggtgca gctgcatgcc
     1861 ggtgttggcg cacagccgaa tgatgttacc ggtgttgggt ggaatttcag gttgataaag
     1921 cacgatatgc agcatggaag aaccggttgg cagttttgta tcgaaaaacg ctgagcatgc
     1981 agggctaagc gcgtgagttc aatcacaaat tgaaatagtc gtctactctt tctaggaaga
     2041 aggtggccgt ctggccaggg cctggattga atgcatgagt agctggctca aacgattgat
     2101 tacaaaaaag aaaccgcaga gtggtgccgt gctgggcgtg agcctgacac cgcgtggcgc
     2161 gggctggtgc gcgctggata tggcgggagc gacgccggtg ctgacgggaa gcgccgccat
     2221 caacggccgc tttcgtacgc cgctggaagc attcgatgcg ctctgtcagc agcaggcgcc
     2281 gccggcgttg ccctgccatc tcagcctgag tcacgaattc tacaacctgt tgctggtgga
     2341 cgcgccgaat gtgccggatg cggaattgcg tgaagccgtg aagtggcggg tgaaggatct
     2401 gattgcccag ccgattgaga aaatggtggt ggatgtgttt cgcctgcccg cagatgcgta
     2461 tcgcggccgc atgaacatgc tttacgcggc attgatcgaa aaggccagtg tgagcccgct
     2521 ggtggtggcc tgcgagcgcg aacatctgag gctggaaggc attggcgttg ccgagctggc
     2581 gctggctgcc gtcacccgtc agctgtccgg cgtcgaaaac ctgagcctgg cactgctgca
     2641 catgaccggt gacaccggtt ccatcaatct ggtggaaaat ggatatctct atctgacccg
     2701 cacgctggag atgaacgcgg gcggcggtta ttcgggaaat gcggactttc agcccgatcc
     2761 taccgacaat ctggcgctcg atatccagcg ttccctggat tattacgaaa gccagttggg
     2821 caagtccggt gtcaatcgcc tgtacctggt gaccgaatcc gaacagcaac agctgtggtg
     2881 tgaaagcctg agccagcgcc tgccgatccg tgcgcatctg tttccccttg ccgatctgtt
     2941 cggcatggac ccgtcgctgg atccgcgaca gcagggcctg ctagtgcccg ccgtgggcgc
     3001 agccctggga ggccggcatg gcggcacttg agcagcgcat caatttctac caggacattt
     3061 ttcacaagcc tgaaattcgc tactcgttcc gtcagttgac gctgattctg ctggtggtga
     3121 tggcgctact ggtggcgctg accacactgg attatgtgcg cacggaaatg cagcgccggc
     3181 acgtggcgca actgcaggct acccaggtga gactggaaaa agccgtggtg gcgatgacgg
     3241 aacagctggg ccggatggtg gctgatcctg cgctggaaaa acaggaagtc tatctgcgtg
     3301 aatcgctgca ggccaaatat cagtttctgg cgcagttgcg cggccagagc gacagccacc
     3361 aggtgcactt ttccgatgtg ctggccggat tgtcggcgtt g
//
LOCUS       DASUEG010000470         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1284596, whole genome shotgun sequence.
ACCESSION   DASUEG010000470 DASUEG010000000
VERSION     DASUEG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284596"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFX11_08750"
     CDS             <1..364
                     /locus_tag="VFX11_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX4938749.1"
                     /translation="IAYGLGWPRRKQSGVPLLDLIKVGRLDFQAPDEARFPCLRLARE
                     AMQTGQTASVVLNAANEIAVEAFLAGQLPFPAIARTLDRVMEKAALVEPIDLDTILKA
                     DAEARALAREQLVQYRKH"
     gene            391..1755
                     /gene="rseP"
                     /locus_tag="VFX11_08755"
     CDS             391..1755
                     /gene="rseP"
                     /locus_tag="VFX11_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX4938750.1"
                     /translation="MYSLMQTVLAFVVTLGVLIFVHEFGHFWVARRCGVRVLRFSIGF
                     GQPLLKWKDSQDTEYVIAALPLGGYVKMLGEPGSEVSEHLKSQSFAHKTVFQRFAIVA
                     AGPLVNLMFAVVLYWLLFLSGITSLAPVVGNVIPGSPAEKAGFQKMEEVVAVDGRATE
                     SWDDIVLELVARLGESTAVRFSIKPEGSAVTDERVIQLDNWMAGQEKENPLKLLGLEP
                     FFPTAPPVLGELEKGMAAERQGLQVGDRILAVNDVELLSWDEWVSLIRQNPMVAMNVK
                     VARGEQELILAVTPDAVMDEKLGRIGRIGAKPDPASVKYPEGMLRELQYGPLDAIPRA
                     LDYTWSRIALTVSAIGKMAMGKISLDNISGPITIAKVAGDSASYGLEPFLTFVAYLSI
                     SLGVLNLLPIPVLDGGHLMYYLVEMVKGSPVSEKAQAFGNSLGLGLLVMFMGLAFYND
                     LMGL"
     gene            1855..>2910
                     /gene="bamA"
                     /locus_tag="VFX11_08760"
     CDS             1855..>2910
                     /gene="bamA"
                     /locus_tag="VFX11_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX4938751.1"
                     /translation="MAETFVIKDIRIDGLQRISAGAVFNSLPLQVGDEVDDATLARSA
                     RLLFKTGNFQDIKLEREGDVLVILVSERPSITKIELEGNKSIETENLMDGLSKAGLSE
                     GSVFQRATLDRLKLELERQYIAQGRYGAKVVPEVTPLPRNRVSIHITIKEGDVATISG
                     VDVVGNTVFTDEELVEDFELKKTHLLSFFKGDDKYSREKLSGDLEKLVSYYQDRGYVN
                     FRVESTQVSVTPDKAEVYISVNVSEGERHTVNEVKLSGELKVPEAELRRLILLKPGQT
                     FSRQLVTTSSELISKRLGNEGYTFANVNGVPQPNDEDKTVAITFVVDPGKRAYVNRIN
                     FRGNTKTEDEVLRREMRQ"
BASE COUNT          590 a          834 c          884 g          602 t
ORIGIN      
        1 gattgcctac ggcctgggct ggccccggcg caaacagtcc ggcgtgccgc tgctggatct
       61 gataaaagtg gggcggctgg atttccaggc gccagacgaa gcgcgctttc cctgcctgcg
      121 actggcgcgg gaagccatgc agaccggtca gactgcgtcc gtggtgctca atgccgccaa
      181 cgaaatcgcg gtggaagcct tcctggcagg gcagctcccg tttcccgcca ttgcccgcac
      241 gctggacagg gtgatggaaa aagcggcatt ggtggaaccg attgaccttg atactattct
      301 aaaggccgat gcagaagccc gcgcgcttgc ccgcgagcag ctggtgcagt accggaaaca
      361 ttgacaccat aacaataact cgaggttgcc ttgtacagct tgatgcagac agtactggcc
      421 tttgtcgtca ccctcggcgt cctcattttc gttcatgagt tcggccattt ctgggtcgcc
      481 cgccgctgtg gcgtcagagt gctgcgtttt tccatcggtt tcggccagcc tttgctcaaa
      541 tggaaagaca gccaggatac cgaatatgtc atcgcggccc tgcccctggg cggttacgtc
      601 aagatgctgg gggagccggg ctctgaagtg tccgaacatc tcaaatccca gtcctttgcc
      661 cacaaaaccg tatttcagcg ctttgccatc gtagcggcgg ggccgctggt gaatctgatg
      721 ttcgcagtcg tcctgtactg gctgttgttc ctgtccggca ttacgtctct cgctcctgtc
      781 gtgggcaatg tcattcccgg cagcccggca gaaaaggccg gtttccagaa aatggaggaa
      841 gtggtggcgg tcgacggccg tgccactgaa agctgggatg atatcgtgct ggaactggtg
      901 gctcgtctgg gggaaagcac ggccgtgcgt ttcagcatca agcctgaagg cagcgcagtc
      961 acggatgagc gcgtgatcca gctggacaac tggatggccg ggcaggagaa ggagaatccg
     1021 ctcaagctgt tgggcctgga accgtttttt cctactgcgc cgccagtgct gggcgagctg
     1081 gaaaaaggca tggccgccga gcgccagggc ctgcaggtgg gtgaccgcat cctggcggtg
     1141 aatgacgtgg agctgctgtc ctgggacgag tgggtgagcc tgatccgaca gaatcccatg
     1201 gtggcgatga acgtcaaggt ggcccgcggg gaacaggagc tgattctggc ggtcacaccc
     1261 gatgcggtta tggacgagaa gctgggccgg atcggccgca ttggtgccaa gcctgacccg
     1321 gcatcggtga agtatccgga aggaatgctg cgtgaattgc agtacgggcc tctggatgcc
     1381 attcccaggg ccctggatta cacctggtcc cgcattgcgt tgacggtgtc tgcgattggc
     1441 aaaatggcca tgggcaagat ctccctcgac aacatcagcg ggccgatcac catagcgaaa
     1501 gtggcgggtg acagcgccag ctacgggctg gagccttttt taaccttcgt ggcctattta
     1561 agtatcagcc ttggggtgtt aaatttgctg cccattccag tactggatgg cgggcacctg
     1621 atgtactacc tcgtcgaaat ggtgaagggc agtccggtct ccgagaaagc ccaggccttc
     1681 ggcaacagcc tgggactggg cctgctggtc atgttcatgg gtcttgcctt ctataacgat
     1741 ttgatgggtc tttgagcccg agccttttca accccctgcg gatttgattg cctgatgatt
     1801 cgatttgctg cattggtagc gctgtggttc ctgcttttat ccgcacccac agccatggcg
     1861 gaaacctttg tgatcaagga tatccgcatc gatggtctgc agcgtatttc cgctggtgcc
     1921 gtgttcaatt ccttgcccct gcaggtagga gatgaagtgg acgatgccac cctggcccgc
     1981 tccgcccgcc tgctgttcaa gaccggcaat ttccaggaca tcaagctgga gcgtgaaggc
     2041 gatgtgctgg tgatcctggt gagcgagcgc ccctccatca ccaagatcga gctggaaggc
     2101 aacaaatcca tcgaaaccga gaatctcatg gatggcctgt cgaaggccgg cctcagcgag
     2161 ggcagcgtgt ttcagcgcgc caccctcgac cggctcaagc tggaactgga gcggcagtac
     2221 atcgcccaag gccgctacgg cgccaaggtg gtaccggaag tcacgccgct gccccgcaac
     2281 cgcgtttcca tccacatcac catcaaggaa ggtgacgtgg ccaccatttc cggcgtcgat
     2341 gtcgtgggca ataccgtctt caccgacgaa gaactggtgg aagatttcga gttgaagaaa
     2401 acccacctgc tttccttctt caagggcgac gacaagtatt cccgggaaaa actgtctggc
     2461 gatctggaaa agctggtgtc ctactaccag gaccgtggct acgtaaattt ccgcgttgaa
     2521 tccacccagg tgtcggtcac cccggacaag gccgaggtat acatctcggt caacgtcagt
     2581 gaaggtgagc gtcacaccgt caacgaggtc aagctcagtg gtgagctgaa agtgccggaa
     2641 gcggaattgc ggcgcctgat cctgctcaag cccgggcaaa ccttctcgcg ccagctggtc
     2701 accacctcca gcgaactgat ttccaagcgt ctgggcaacg agggctacac cttcgccaac
     2761 gtcaacggcg tgccgcagcc caacgatgaa gacaagaccg tcgccatcac cttcgtggtc
     2821 gatccgggca agcgtgccta cgtcaatcgc atcaacttcc gcggcaacac caagaccgag
     2881 gacgaggtgc tgcgccgcga gatgcgccag
//
LOCUS       DASUEG010000471         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_22858, whole genome shotgun sequence.
ACCESSION   DASUEG010000471 DASUEG010000000
VERSION     DASUEG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22858"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFX11_08765"
     CDS             complement(<1..264)
                     /locus_tag="VFX11_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX4938752.1"
                     /translation="MNPLRFSGRKQVPLLQQTEAAECGLACLAMVATYHGFDTDLPSL
                     RRRFAVSAQGMTLAQLMEFSGRMDLCARPLRVELEALNQLTLPA"
     gene            complement(261..1508)
                     /locus_tag="VFX11_08770"
     CDS             complement(261..1508)
                     /locus_tag="VFX11_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4938753.1"
                     /translation="MTASLFRAEAIAHQRNARMGDVLSVRTAPLAWLTGLFVLLALAL
                     LLFLGFGEYTRKARVVGFLAPSQGMVKVLATQAGTVIEKRVNEGQQVKQGDVLFVLAA
                     EQASLESPDARAAAITNIVARRDSLLDELEKQDDLNRIRLQDRRDRATTLAGELRQLD
                     QEIQLRQQRLASAQASVDKFRDLYARQFVAELQMREKQDELLEQDSALQALKRNRLTL
                     ERELGGLQAEIDSSKYEFARQRAELDRAISRQEQELTEYQARRSIVITAPASGTATAI
                     LLQKGQTAAAQAPLLSILPQGSDLQAHLLVPSRSIGFIVKGQAVALRYAAFPYQRFGS
                     QPGEVSEISQNLIAPNETDFPVKLDEPAYRVVVRPARQTLQAYQNAIPLQPGMLLDAD
                     VKLERRRLFQWLFDPLYSLAGTL"
     gene            complement(1510..1944)
                     /locus_tag="VFX11_08775"
     CDS             complement(1510..1944)
                     /locus_tag="VFX11_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein"
                     /protein_id="HEX4938754.1"
                     /translation="MNADPKPHCPSAQPEWAGAKVFGVVGGTPDAPATAYLAAPAPVT
                     PELLAAAEPVRPDEVFRFAAPCACSGCSHFAEADSKCRLVQKIVRWVPVVTDSLPVCS
                     IRAECRWWQQEGRAACMRCPQVVTNDFHPTPAIRNAANPELF"
     gene            complement(1948..2226)
                     /locus_tag="VFX11_08780"
     CDS             complement(1948..2226)
                     /locus_tag="VFX11_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938755.1"
                     /translation="MNTVPMKPQRITHSEIASVAAEGVARALAAREEHLRELSPGEID
                     AVGGGALMLKYQGLINGGRVIDALAGLGGLATNPAADLGGLSAGFGMV"
     gene            complement(2273..2452)
                     /locus_tag="VFX11_08785"
     CDS             complement(2273..2452)
                     /locus_tag="VFX11_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938756.1"
                     /translation="MQMETTKQPAVQELDLEECRQVGGGNLSPDQDISDFPVIALPRI
                     NVAINAKLAKVAVLG"
     gene            2811..>3225
                     /locus_tag="VFX11_08790"
     CDS             2811..>3225
                     /locus_tag="VFX11_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938757.1"
                     /translation="MFEHKELDGEKHRVLPASEPFILDEVLELRVPRNDKPVTTVGRH
                     QHGVAGGVLVERYLPPETVGDSWQHDPGTPWWEVVGHPPHGGIVADFNRAKGGAYRRA
                     LVPDRNVVAYAAAGEEVLLQGAEYKVVRKTDGQELK"
BASE COUNT          644 a          994 c          986 g          601 t
ORIGIN      
        1 cgccggcagc gtcagctggt tcagcgcttc cagttccacc cgcaacggcc gcgcgcacaa
       61 atccatccgg ccggaaaatt ccatcagctg cgccagcgtc atgccctggg ccgacacggc
      121 gaaacgtcgc cgcaggctgg gcagatcggt gtcgaagccg tgataggtgg ccaccatcgc
      181 cagacaggcc aggccacact ctgccgcttc ggtctgctgc agcagcggta cctgtttgcg
      241 accggaaaaa cgcagcggat tcacagcgtg cccgccagac tgtagagcgg atcgaacagc
      301 cactggaaca gccggcgccg ctccagtttc acgtcggcat ccagcagcat gcccggttgc
      361 aacggaatgg cattttggta tgcctgcaac gtttggcgcg cgggccgcac caccacgcga
      421 taagccggtt catccagttt caccggaaaa tccgtttcgt tgggcgcgat caggttctgc
      481 gaaatttcgc tgacctcgcc gggctggctg ccgaaacgct gatacggaaa agcagcgtaa
      541 cgcagcgcca cggcctgacc tttgacgata aaaccaatgg agcggctggg caccagcaga
      601 tgggcctgca gatcggaacc ttgtggcagg atcgacagca acggcgcctg tgcggcagca
      661 gtctggcctt tttgcagcag gatcgcggtg gcagtgccgg acgcaggcgc ggtgatcaca
      721 atgctgcgac gagcctggta ttcggtgagt tcctgttcct ggcgggaaat ggcgcggtcc
      781 agctcggcgc gctggcgggc gaattcatat ttgctggaat cgatttccgc ctgcaggcca
      841 cccagttccc gctccagtgt caggcgattg cgtttcagtg cctgcagcgc gctgtcctgc
      901 tccagcagtt catcctgttt ttcccgcatc tgcagttccg ccacaaactg gcgtgcgtat
      961 aaatcccgga atttatcaac gctggcctgg gcactggcca gacgctgctg acgcagttgt
     1021 atttcctgat ccagctggcg cagttcaccg gccagtgtgg tggcacgatc gcgccggtcc
     1081 tgcagacgga tgcgattgag atcatcctgc ttttccagtt cgtccagcag actgtcgcgc
     1141 cgcgccacga tattggtgat ggccgctgcg cgggcatcgg gactttccag cgacgcctgt
     1201 tctgcggcca gtacaaacag cacatcgccc tgcttcacct gctggccttc gttcacccgt
     1261 ttttcaatca cggtgcccgc ctgggtggcc agcactttca ccatgccctg actgggcgcc
     1321 agaaacccca cgacccgcgc cttgcgggtg tattcaccaa atcccagaaa aagcagcaac
     1381 gccagcgcca gcaacacgaa caggccggtg agccaggcca gcggcgcagt gcgtaccgac
     1441 agcacatcgc ccatcctcgc attgcgttga tgggcgatgg cctccgcgcg aaacaatgat
     1501 gctgtcatgt cagaacagtt ccggattggc ggcgttgcgg atggcaggcg tcgggtggaa
     1561 atcattggtc accacctgtg gacaacgcat gcacgcagcc cgcccttcct gctgccacca
     1621 gcgacattcg gcgcggatac tgcacaccgg cagactgtca gtcaccaccg gcacccagcg
     1681 cacgattttc tgcaccagac ggcatttgga atccgcttcg gcaaaatggg agcaaccgct
     1741 gcaggcacag ggcgcggcaa agcggaacac ttcgtcgggc cgcacgggtt ctgcagcggc
     1801 cagcaactcc ggcgtcaccg gtgcgggtgc cgccagatac gccgttgccg gcgcatccgg
     1861 ggtaccgccg accacaccga acaccttggc gcccgcccac tccggctgag cactggggca
     1921 atggggttta ggatcggcat tcatggttca caccatgccg aagccggcgc tcaagccgcc
     1981 cagatcggcc gccggattgg ttgcaagacc acccaaaccg gcgagagcat cgatcactct
     2041 gccgccattg atgaggccct gatatttcag catcagcgcg ccgccaccga cagcgtcaat
     2101 ttccccgggg ctcagttcac gcagatgttc ctcccgcgcc gccagcgccc gggccacacc
     2161 ttcggcggca acagacgcaa tttcggaatg ggtaatgcgt tggggtttca tgggtactgt
     2221 gttcatgtcg ttctcctgat cggaaaaaat gttcaacgca gcagatgggc tatcagccca
     2281 gtactgccac tttggcgagc ttggcattga tggcaacgtt gatgcgcggc aaggcaatca
     2341 cgggaaaatc cgagatgtcc tgatccggcg acaggttgcc accgccgacc tgacggcact
     2401 cttcgaggtc cagttcctgt accgcaggtt gttttgttgt ttccatttgc atgaagtttt
     2461 ctcctggcga gttgatcgtg aatgtgagca cgtcgcggca aaattttttg cacggactcc
     2521 gccgccattg ctgcgacagg cccaaggcta cgcccgccgg atgcatccag gttgttagaa
     2581 gctgaacctt gcaaaagcgg atttgtggac gcctgcaatg caaccggcac acatttaaaa
     2641 gaatggttta cgacagaaag aagccgcatt gcggcggcag ggaatggcgc tgcaataccg
     2701 ctgcgcaggg cggaataatg cagccgctgt cgccggaata aacggccgga tcgacggcac
     2761 ggcacttgca atcacatccg caccgcaacc ttttttgtag gagatccgac atgtttgaac
     2821 acaaggaact ggatggggaa aaacaccgcg tgctgccggc aagcgagccg ttcattctgg
     2881 acgaggtgct ggaactgcgc gtgccgcgta acgacaaacc ggtgacgacc gtcggccggc
     2941 atcagcacgg cgttgccggc ggcgtactgg tggaacgcta cctgccaccg gaaaccgtgg
     3001 gcgacagctg gcagcacgac ccgggcacgc cctggtggga agtggtgggc catccgcccc
     3061 acggcggcat cgtggcggat ttcaatcggg cgaaaggtgg ggcttaccgg cgggcactgg
     3121 tgccggaccg caatgtggtg gcctatgcgg cggctggcga ggaagtgctg ttgcagggag
     3181 ccgagtacaa ggtggtgcgc aagactgatg ggcaggaatt gaagg
//
LOCUS       DASUEG010000472         5701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_7313, whole genome shotgun sequence.
ACCESSION   DASUEG010000472 DASUEG010000000
VERSION     DASUEG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7313"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /gene="groL"
                     /locus_tag="VFX11_08795"
     CDS             complement(<1..1643)
                     /gene="groL"
                     /locus_tag="VFX11_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253075.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX4938758.1"
                     /translation="MAAKEVKFGDAARVKMVKGINILADAVKVTLGPKGRNVILEKSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASQANDAAGDGTTTATVLAQAIVTE
                     GLRAVAAGMNPMDLKRGIDKAAAAVVDQLQSLSTPCADSKSIAQVGTISANSDTSVGS
                     IIAEAMEKVGKEGVITVEEGSGLENELEVVEGMQFDRGYLSPYFINNQEKMCVDLDNP
                     FILLVDKKISNIRDMLPILEAVAKSGKALLIIAEDVEGEALATLVVNNMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLDGATLNDLGTAKKVSITKENSTIVD
                     GAGHESEIQARVKQIRQQIEEATSDYDKEKLQERVAKLAGGVAVIKVGAGSEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALLRALATVGSLKGDNDDQTAGIQLALRAMESP
                     IRQIATNAGAEASVVVDKVKAGKGNYGYNAATGEYGDMIEMGILDPAKVTRSALQAAA
                     SIAGLMITTEAMVAEIPEDKPAGPDMSGMGGMGGMGGMM"
     gene            complement(1703..1993)
                     /locus_tag="VFX11_08800"
     CDS             complement(1703..1993)
                     /locus_tag="VFX11_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX4938759.1"
                     /translation="MKIRPLHDRVVVRRVEEETKTAGGIVLPGSAAEKPSQGEVVAVG
                     PGRLTEHGEVRPLDVKAGDKVIFGQYAGSTVKIDGKELLIMAESEIFGVLES"
     gene            complement(2301..2813)
                     /locus_tag="VFX11_08805"
     CDS             complement(2301..2813)
                     /locus_tag="VFX11_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX4938760.1"
                     /translation="MRLILFLFVFVMLEAMVAARVAQLAGWGTTLLLLLSTSLLGSYL
                     VQRQGLSTWVRLNQRMLAGEAPGVELVEGLLLLTGGFLLIIPGFITDALGLFLLLPPF
                     RRALAALLIRRGLLQALARGGQSGTWVYSARYQRSASADAEPEPGRPRVQEDRNGHLV
                     IEGEAERKDE"
     gene            complement(2826..3566)
                     /locus_tag="VFX11_08810"
     CDS             complement(2826..3566)
                     /locus_tag="VFX11_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX4938761.1"
                     /translation="MDKQGGGELESAAAQILQAYFSGVLSTHSVEFPGYPFGSVVPFC
                     LDESGQPLILISALAQHTRNIVANPKVALTLLDAGEGDVQQDARLTLLADVEAVADSA
                     IASCATRYYRHFPEASGYHDQLDFRFYRLQVQRLRYIGGFGKIRWLSPGAVLLPNPFA
                     PEREADMVGHMNEDHASAILHYCDLAGIPHQDRAVTMAGIQANGMVLRVGARLHFIAF
                     GRSVATPDEARAVLVEWARRPLPGPDGK"
     gene            3727..4542
                     /locus_tag="VFX11_08815"
     CDS             3727..4542
                     /locus_tag="VFX11_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938762.1"
                     /translation="MGTRLAMLVLALPLQAAEPEPSPLQQKEPANYAFAAYMGSGLYS
                     ASDQSLFVLNIPTTWDIPDHPSLRFRLTFSAGFFNYGAEEIMDLDVPDEMGTLTVIPG
                     IEKWFALNDRLNLIPHLDYGFARNMSTGEEAQVYSVGVRTEYHFDGMTSQNIWVNKLV
                     YAGSRTFESDQQDTYVQLLTGFDYSLGNGFTLWEHNVRPTFYALTYWSHNGIDYIERW
                     QDGTSDEMVFELGSTLWAPEPYTIMMMDVDRIGIGYQQNAFGHVFRLFAGSVF"
     gene            4675..5436
                     /locus_tag="VFX11_08820"
     CDS             4675..5436
                     /locus_tag="VFX11_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938763.1"
                     /translation="MELKNKVVVITGAGRGLGRGMALAFAAKGANVGVVDLNEDDIRQ
                     TVALCEEKGVKAKGYKVNVAKEEEVEKLFGDVVADFGALHGLVNNAGITRDGLLLKFK
                     DGELQGKMSLQQWQAVIDVNLTGVFLCGREAAAKMLQCGVEEGVIVNISSLARHGSFG
                     QTNYSAAKAGVVAMTEAWAKELSRFNIRTGAIAPGTINTDMIAAMKPDARDRLVSAVP
                     LKRLGEPDNIAKAAIFIFENDYFTGRVVEVDGGLR"
BASE COUNT         1097 a         1700 c         1709 g         1195 t
ORIGIN      
        1 tacatcatgc cgcccatgcc acccattcca cccatgccgc tcatgtcagg gccggcgggc
       61 ttgtcttcgg ggatttcagc taccatggct tcggtggtga tcatcagacc ggcaatggaa
      121 gcagccgcct gcagcgcaga acgggttact ttggccgggt ccaggatacc catttcaatc
      181 atgtcgccgt attcaccggt ggctgcgttg tagccgtaat tgcccttgcc tgccttgacc
      241 ttgtctacca ccacggatgc ttccgcaccg gcgttggtcg cgatctggcg gatcggggat
      301 tccatggcgc gcagtgccag ctggatacca gcggtctggt catcgttgtc gcctttcagg
      361 ctgccaacgg tggccagggc acgcagcaga gccacgccac cgcctgcaac tacgccttct
      421 tcaacagcgg cgcgggtagc gtgcagagcg tcttcaacgc gggctttctt ctctttcatt
      481 tccacttcag aaccggcgcc aaccttgatc acggcaacac cgccagccag tttggccacg
      541 cgctcttgca gtttttcttt gtcgtagtcg gacgtggctt cttcgatctg ctgacggatc
      601 tgcttcacgc gggcctggat ttcagactcg tggccagcgc cgtcaacgat agtggagttt
      661 tccttggtga tgctgacttt cttggcagtg cccagatcgt tcagggtggc gccgtccagg
      721 gacagaccta cttcttcgga gatcacggtg ccgccggtca ggatcgcgat gtcctgcagc
      781 atggccttgc ggcggtcgcc gaagccaggg gctttaaccg cagccacttt cacgatgccg
      841 cgcatgttgt tcaccaccag agtggccagc gcttcgcctt ccacgtcttc tgcgatgatc
      901 agcagggcct tgccggattt ggcaacggct tccaggatcg gcagcatgtc gcggatgttg
      961 gagattttct tgtcaaccag caggatgaac gggttgtcca ggtcaacgca cattttttcc
     1021 tggttgttga tgaagtaagg ggacaggtag ccgcggtcga actgcatacc ttcaaccact
     1081 tccagttcgt tttccaggcc gctgccttct tcaacggtga tcacgccttc tttgcccact
     1141 ttttccatgg cttcagcaat gatgctgccc acggacgtgt cggagttggc agagatggtg
     1201 cctacctggg cgatggactt ggaatcagcg cagggagtgg acagggactg cagttgatca
     1261 accacagcag cagcagcctt gtcgatgccg cgcttcaggt ccatcgggtt catgccggcg
     1321 gctacagcgc gcagaccttc ggtcacgatt gcctgtgcca gaacggtggc agtggtggta
     1381 ccgtcaccgg ccgcgtcgtt ggcctgggaa gccacttcct tcaccatctg cgcgcccatg
     1441 ttttcgaact tgtctttcag ttcgatttct ttcgcaaccg acacgccatc cttggtgatg
     1501 gtgggtgcgc cgaaagattt ttccaggatg acgttacggc ctttggggcc cagggttact
     1561 ttcactgcgt cagccaggat gttgatgccc ttgaccatct tgacgcgggc ggcgtcgccg
     1621 aatttgactt cttttgctgc catgttcgct attcctttga attctgtttc agtaatggat
     1681 cgggtgcaga ttgcggaagc gcttagcttt ccagcacacc gaagatttcg ctttcagcca
     1741 tgatcagcag ttccttgccg tcgatcttga cggtgctgcc tgcgtactgg ccgaagatca
     1801 ccttgtcacc ggctttcaca tccaggggac gcacttcgcc gtgctcggtc agacggccgg
     1861 ggccaacggc aaccacttca ccctgggaag gcttttcagc agcggagcca ggcagaacga
     1921 taccgccagc agttttggtt tcctcttcta cgcggcggac cacgacgcga tcatgcaacg
     1981 gacggatttt catgggtctt taatctccat ttagctcatt gttcttggct tgggttggcg
     2041 gcgccggcct gcagcatgca agcaccggcc tgccagggcc gataaacgat cgtgcacttt
     2101 aattagcact ctcgcctctg gagtgccaat aatagtgacg tggttgaatc cgtcaagact
     2161 tctcttgggt ttaggtgccc gcgaggggtc tttacgacag gatgcccgct agatggggcc
     2221 cgcagggggc atttcaaggg cggaaagggt gaaattttgc tgacaggcgg gaattcggca
     2281 cagcctcggg cctgccggcg tcactcgtcc ttgcgctccg cctcgccttc gatcaccaga
     2341 tggccattgc ggtcttcctg cacccggggt cggccaggct ctggctcggc atcggcgctg
     2401 gcggaacgct ggtaacgggc cgaatagacc caggtaccgg actgtccgcc acgggccaac
     2461 gcctgcaata gcccccgccg gatcagcaga gcggccagtg cacgccggaa cgggggcagc
     2521 agcaggaaca ggcccagggc atcggtaatg aagccgggaa tgatcagcag gaacccgccc
     2581 gtgagcagca gcaggccctc caccagctca acgcccggcg cctcgcccgc cagcatgcgc
     2641 tggttcagcc gcacccaggt ggacaggccc tgccgctgca ccaggtaaga ccccagcagc
     2701 gatgtggaca gcaacagcag cagcgtggtg ccccagccag ccagctgggc tacccgcgcc
     2761 gccaccatgg cctccagcat cacgaataca aacaggaaca ggatcagacg cactgggtta
     2821 ctcctttatt taccatccgg acccggcagc gggcgccgcg cccattccac cagcaccgcg
     2881 cgggcttcgt ccggcgtggc cacactgcgt ccgaacgcaa tgaagtgcag ccgcgcgccc
     2941 acccgcagca ccattccgtt ggcctggatg ccggccatgg tcacggccct gtcctgatgg
     3001 ggaataccgg ccaggtcaca gtaatggagg atggcgctgg catggtcctc attcatatga
     3061 cccaccatgt ccgcctcccg ttccggcgca aacggattgg gcagcagcac cgcgcccggg
     3121 gacagccagc ggatcttgcc gaagcccccg atgtagcgca gccgctgcac ctgcagccgg
     3181 tagaagcgga aatccagctg gtcgtggtag ccgctggcct cgggaaagtg acggtaatac
     3241 cgggtggcgc aggatgcgat ggcactgtct gccacagcct cgacatctgc cagcagggtc
     3301 aggcgggcgt cctgctgcac atcgccctcg ccggcgtcca gcagggtcag cgccaccttc
     3361 ggattggcaa cgatattgcg cgtgtgctgg gccagagcgc tgatcaggat caggggttgc
     3421 ccgctttcat ccaggcagaa tggcaccact gaaccgaagg gatatccagg aaattccacc
     3481 gagtgtgtgg agagcacacc ggagaagtag gcctgcagta tctgcgccgc ggcggattcc
     3541 agctcaccgc caccctgttt atccatcgcc tgtttatcca tagaatgggc ccccattgat
     3601 tccagccgac atcttaacaa ttcgcatgca catcagtaac tccagcgcag gaaaccgggt
     3661 gtgatcgcca gagcccgatc ggcgcatcgc aaagaaaggc gcagactggc aaggtcggtt
     3721 tccgacgtgg gcacacggct ggccatgctg gtacttgcgc tgccactgca ggcggccgag
     3781 ccggagccat ccccgctgca acagaaggaa cctgccaatt atgcattcgc ggcctacatg
     3841 ggcagcggtt tgtattcggc gtcggaccag tcgctgttcg tgctgaacat tcccaccacc
     3901 tgggacatcc cggatcaccc cagtctgcgc ttccggctga cgttttccgc cggttttttc
     3961 aattacggtg cggaggaaat catggatctg gatgtgcccg acgaaatggg taccctcacg
     4021 gtgattcccg gcattgagaa atggtttgcc ctgaacgaca ggctcaatct gattccgcac
     4081 ctggactatg gctttgcccg caacatgtcc actggcgagg aggcccaggt ctactccgtg
     4141 ggcgtacgta cggaatatca tttcgacggc atgaccagtc agaatatctg ggtcaacaag
     4201 ctggtatatg cagggtcgcg caccttcgag agcgaccagc aggacaccta cgtgcaattg
     4261 ctgacgggct tcgactacag cctgggcaat ggcttcacgc tgtgggagca caacgttcga
     4321 cccacctttt acgccctcac ctattggagc cataacggca tcgactatat cgagcgctgg
     4381 caggacggca ccagcgacga aatggtgttc gagcttggct caaccctgtg ggcgccagag
     4441 ccctacacga tcatgatgat ggatgtcgac cggatcggca tcggctacca gcagaatgcg
     4501 ttcggccatg tgttccgcct gtttgccggt tcggtgttct gacctgtccg ccgcgccgct
     4561 ggcgttcagc aggcaaaacg ggaataatcg cgactcctta gtctatcggg caactcactc
     4621 cgttgagtca acccgtcccc attccaattt ttcctgtatc agagggcagc agttatggaa
     4681 ctgaagaata aagtggtcgt cattaccggc gcgggccggg gcctgggtcg tggcatggcg
     4741 ctggcgttcg ccgccaaggg tgccaacgtg ggcgtggtgg acctgaatga agacgatatc
     4801 cgccagactg tggccctgtg cgaagagaag ggcgtgaagg cgaagggcta caaggtgaac
     4861 gtggccaagg aagaggaagt ggagaagctg ttcggtgacg tggtggcgga tttcggtgcg
     4921 ctgcacggcc tggtgaacaa tgcgggcatc acccgcgatg gtctgctgct gaaattcaag
     4981 gacggtgagc tgcagggcaa gatgagcctg caacagtggc aggcggtgat cgacgtaaac
     5041 ctgaccggcg tgtttctgtg cggccgtgaa gcggcggcga aaatgctgca gtgcggcgtg
     5101 gaagaaggcg tgattgtgaa catttccagc ctggcgcgtc atggcagctt cggccagacc
     5161 aactactccg ctgcgaaagc cggcgtagtg gcgatgacgg aagcctgggc gaaggaactg
     5221 tcccgcttca atatccgcac cggtgcgatt gcacccggca ccatcaatac cgacatgatc
     5281 gcggcgatga aaccggatgc tcgggatcgt ctggtcagcg cggtgccgct gaaacgtctg
     5341 ggcgagccgg acaacattgc aaaagcagcg atcttcattt ttgaaaacga ctacttcaca
     5401 gggcgtgtag tggaagtgga tgggggcttg cggtaaattt tactgcgccg ctggtatatt
     5461 cgtactccgc atcccgtgca cagccaaacg gagcaggggg cggtaaatcc agacacgcca
     5521 cgagtacgtc cctgtaggct cgacagcggc catccatggc cgctgacgtt ctggatttac
     5581 cgccccctgc tccgttcgtc tgtacactaa cttctgtgca acctcttaag cattgtggtg
     5641 cctgatgttc gcagagttgg agcgagcgca gaactacttc gggacttgtt gtggtccagt
     5701 g
//
LOCUS       DASUEG010000473         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1547192, whole genome shotgun sequence.
ACCESSION   DASUEG010000473 DASUEG010000000
VERSION     DASUEG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547192"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFX11_08825"
     CDS             complement(<1..345)
                     /locus_tag="VFX11_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336014.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4938764.1"
                     /translation="MIDLHGFGPALAAGTLMTIKLALCALVLGLVLGLAGALAKTSSS
                     RILVYIGGLYSTVVRGIPELLWVLLMYFGTVNAMRALGDWLGRPELELSPFAAGVLAL
                     GLCFGAYATEVFR"
     gene            complement(412..1164)
                     /locus_tag="VFX11_08830"
     CDS             complement(412..1164)
                     /locus_tag="VFX11_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4938765.1"
                     /translation="MNNCKKILLALAAAFALVSQAAVADKLRIGTEGAYPPFNLTDAS
                     GKVVGFDMDIAHALCAKMKAECEVVTSDWDGIIPALNAKKFDFIVASMSITDERKQAV
                     DFTDPYYTNKLQFIGPKSAKVAVDKAGLKGKAVGTQRATIAGEWLEKNMGDAVDLKLY
                     DTQENAYLDLSSGRIDLVLGDVFVNWGWLKSDAGKDFEFKGEPVFDNDKIGIAVRKND
                     PLREKLNAALKEIIADGTYKKINDQYFPFSIL"
     gene            complement(1179..1952)
                     /locus_tag="VFX11_08835"
     CDS             complement(1179..1952)
                     /locus_tag="VFX11_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938766.1"
                     /translation="MIEAAPALEIRNLHKRYGDLEVIKGISLTARDGDVISILGSSGS
                     GKSTFLRCINLLESPHQGQIIVAGEELKLKPSASGNLVAADSKQINRLRSKIGFVFQN
                     FNLWPHMTVLDNVTEAPRRVLGMSKAAATEMGEALLAKVGIADKRHCYPNQLSGGQQQ
                     RAAIARTLAMQPKVILFDEPTSALDPEMVQEVLNVIRALAEEGRTMLLVTHEMNFARQ
                     VSSEVVFLHQGMVEEKGTPDAVFNHPKSERCKQFLSSNH"
     gene            2211..>2984
                     /locus_tag="VFX11_08840"
     CDS             2211..>2984
                     /locus_tag="VFX11_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007104171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4938767.1"
                     /translation="MKAAFHWEDPFLLQQELSADERAIQDTARQYAQDKLLPRVVLAN
                     RTERFDRDIYREMGALGLLGSTLPPQYGCAGVNHVAYGLIAREIEKVDSAYRSTLSVQ
                     SSLVMFPIFEFGDEAQRQHYLPKLASGEWVGCFGLTEPDHGSDPGGMKTRAEKIPGGY
                     KLNGEKLWITSSPIADVFVVWAKLDGVIRGFVLERGLKGLSTPKIEGKLSLRASVTGG
                     IVLQDVEVPETALLEKAKGLAGPFSCLNKARYGIAWGTLG"
BASE COUNT          619 a          928 c          834 g          603 t
ORIGIN      
        1 gcgaaacact tcagtggcgt aggcgccaaa gcacagccca agcgcgagca caccggcggc
       61 gaacgggctc agttccagct ccggccggcc cagccagtca cccagcgcgc gcatcgcatt
      121 cacggtgccg aaatacatca gcaacaccca cagcaactcg ggaataccgc gcaccacagt
      181 cgaatagagt ccgccgatat agacgagaat ccggctggaa gaggttttgg ccagagcccc
      241 cgccaatccc agcaccagcc ccagcaccag tgcgcacagg gccagcttga tggtcatgag
      301 ggtgccggcg gccagagctg gcccgaagcc atgcaggtca atcatggaaa gcaatcatga
      361 aatagcgccg tcaaaggccg cgcgctgcca gggcagcgcg ggcggggtcg gtcacagaat
      421 gctgaaagga aaatactggt cgttgatttt cttgtatgtg ccgtctgcga taatttcctt
      481 cagcgcggca ttcagtttct cgcgcagcgg atcgttcttg cgcaccgcga taccgatctt
      541 gtcattgtcg aacaccggct cgcccttgaa ttcgaaatcc ttgccggcat cgctcttcag
      601 ccagccccag ttcacgaaca catcccccag caccagatcg atccggccgg acgacaggtc
      661 cagatacgca ttttcctggg tgtcgtacag tttcagatcc actgcgtcac ccatgttttt
      721 ttccagccac tcaccggcaa tcgtcgcgcg ctgggttccc accgccttac ccttcagacc
      781 ggccttgtct accgccactt tcgcactttt cggcccgatg aactgcagct tgttggtgta
      841 atatggatcg gtgaaatcca ccgcctgctt gcgctcgtcg gtgatcgaca tggacgcgac
      901 gataaaatca aattttttcg cattgagcgc gggaatgatg ccatcccagt cggacgtcac
      961 cacttcgcat tccgctttca tttttgcaca cagcgcgtgg gcgatatcca tgtcgaagcc
     1021 caccaccttg ccgctggcat cggtgagatt gaacggagga taggcgcctt cagtgccaat
     1081 gcgcagcttg tcggcgaccg ccgcctggga caccagggca aacgcggccg ccagggccag
     1141 caggattttt ttgcaattgt tcatgcggaa tgactccatc aatggttgct ggacaggaat
     1201 tgtttgcagc gttctgactt cgggtgattg aacaccgcgt cgggtgttcc cttctcttcc
     1261 accatgccct gatgcagaaa taccacttcg ctcgacacct ggcgggcgaa attcatttca
     1321 tgggtcacca gcagcatggt gcgaccttct tcggccagtg cacgaatgac gttcagcact
     1381 tcctgcacca tctccgggtc cagcgccgac gtcggttcgt caaacagaat gactttgggt
     1441 tgcatggcca gagtgcgggc aatggcggcc cgttgctgct ggccaccgga cagctggttc
     1501 ggataacaat gacgcttgtc ggcaatgccc accttggcca gcagcgcttc gcccatttcc
     1561 gtggccgctg ccttgctcat gcccagcacg cggcgcggcg cttcggtgac gttatcgagc
     1621 accgtcatgt gcggccacag attgaaattc tggaatacaa aaccgatttt gctgcgcagg
     1681 cgattgatct gtttgctgtc ggcggcaacc aggttgccac tggcggaagg tttgagcttc
     1741 agttcctcgc cggccacgat gatctggccc tgatggggcg attccagcag attgatgcag
     1801 cgcagaaagg tggacttgcc ggaaccggaa gaacccagaa tggaaatgac gtcgccgtcg
     1861 cgggccgtca gggaaattcc cttgatcact tcgagatcgc catagcgttt gtgcaggttg
     1921 cgaatttcca aagcgggagc ggcttcaatc atggcttcgg tcatggcgcc tctcgtcggg
     1981 ctctgttcat cggatgacac atccatctgg cagggtgatt gaagaataat cgtcctcggt
     2041 gccggacaca accaccctgg caccaccatc cgggtcaggc tccactggat tgcggcaaac
     2101 gcaccagcat cgcccagtcc aggcgcctgg cgggtgcgtt tagggttttc gcccaaccgt
     2161 gctaaccttt ctgctgcctc ctttagatgc agtacacgga taacaacaag atgaaagccg
     2221 cattccactg ggaagacccg ttcctgctgc agcaggaact gagcgccgac gagcgcgcca
     2281 tccaggacac cgcccgccag tacgcccagg acaaactgct gccccgcgtc gtcctcgcca
     2341 accgcactga acgtttcgac cgcgacatct accgcgaaat gggtgccctg ggcctgctgg
     2401 gttccaccct gccgccgcaa tacggttgcg ccggcgtcaa ccacgtggcc tacggcctga
     2461 ttgcccgcga aatcgaaaaa gtcgacagcg cctaccgctc caccctgagc gtgcagtcgt
     2521 ccctcgtcat gttcccgatt ttcgaatttg gcgatgaagc acagcgccag cattacctgc
     2581 ccaaactggc cagcggagaa tgggtgggtt gcttcggcct caccgaaccg gaccatggct
     2641 ccgatcccgg cggcatgaaa acccgcgccg aaaaaattcc cggcggctac aaactcaacg
     2701 gtgaaaaact ctggatcacc agcagcccga ttgccgatgt gttcgtggtg tgggcgaaac
     2761 tggacggcgt gattcgcggc ttcgtgctgg agcgcggact caagggctta tccacaccga
     2821 aaattgaagg caaattgtcg ctgcgggcat cagtcaccgg tggcatcgta ctgcaggatg
     2881 tggaagtacc cgaaaccgct ttgctggaaa aagccaaagg cctggccggc ccgttttcct
     2941 gcctgaacaa ggcgcgctac ggcatcgcct ggggcaccct gggc
//
LOCUS       DASUEG010000474         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_910865, whole genome shotgun sequence.
ACCESSION   DASUEG010000474 DASUEG010000000
VERSION     DASUEG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910865"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VFX11_08845"
     CDS             <1..166
                     /locus_tag="VFX11_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938768.1"
                     /translation="EDLVGKLTVMVANLAPRKMKFGMSEGMVLAAGPGGKDIFILEPD
                     SGATPGMRVK"
     gene            222..806
                     /gene="rsxA"
                     /locus_tag="VFX11_08850"
     CDS             222..806
                     /gene="rsxA"
                     /locus_tag="VFX11_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX4938769.1"
                     /translation="MPEFLLVLISTILVNNFVLVQFLGLCPFMGVSNKLETAIGMAMA
                     TTFVLTLSSVLTYLTYTYILVPLDVGYLKTMSFILVIAVTVQFTETVMRKTSPLLYRV
                     LGIFLPLITVNCAVLGVALLTVKKGVDSFIYAFLYGFGGALGFSLVLILFAAMRERIL
                     VSDVPTPFKGASIGMITAGLMSLAFMGFSGLIKI"
     gene            817..1413
                     /gene="rsxB"
                     /locus_tag="VFX11_08855"
     CDS             817..1413
                     /gene="rsxB"
                     /locus_tag="VFX11_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEX4938770.1"
                     /translation="MDLLITNIGAVSALVALALAFGAILGYAAVKFHVEGNPIVDQID
                     DLLPQTQCGQCGHPGCRPYAEGMAAGEEINKCVPGGEDTIRALADLLGREFVPLDAEY
                     GEEKQHKMVAHIREAECIGCTKCLQACPVDAILGAAKQMHTVMVDECTGCDLCVEPCP
                     VDCIDMIPVKTTQQTWTWQPPAGIGARPGDLIPVRNIA"
     gene            1427..3661
                     /gene="rsxC"
                     /locus_tag="VFX11_08860"
     CDS             1427..3661
                     /gene="rsxC"
                     /locus_tag="VFX11_08860"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX4938771.1"
                     /translation="MSRRIWDIPGGIHPPENKQQSSRNAIRPAAIPARLVVPVQQHIG
                     APAKPLVQVGDHVLKGQKIGEATGFVSLPVHAPTSGVVEAIELHAVQHPSQIDSLCIT
                     LKTDGEDRWTERQPLADYRAHTKKEVLDRIRNAGIAGMGGAGFPTVVKLGVKADAQIE
                     QLILNAVECEPYITADDRLIQERAAEVVAGLQIMAWLVEPKEILIGIEDNKPQAIEAL
                     RNVCAGLTKQNGATIDVVVVPTKYPSGGEKQLIQILTGKEVRSGGLPADVGVICQNVG
                     TAYAVKRAIIDGEPLISRITTLTGDAIEQKGNYEVLLGTPVADLLQQAGLKKDKLHRL
                     VMGGPMMGFTIHDPAVPVVKTTNCLLVATEQEFPDPAPEQPCIRCASCAQACPVNLLP
                     QQLYWFAKAKEFDKAEHFNLFDCIECGACSYVCPSSIPLVQYYRFAKGAIRHQQQEVH
                     KADRARERFEARKARIEREEQEKELKRQERAKAAAAKQSAKKEAPAGAASTATAPAPT
                     SGAAPASPAATDAAPVADELTKLQTAAASTLKRYKDAQKALEVAEKNGSEHIDALRAK
                     VEQLKTKADAAKDAFIAAKAAEKSAPDSGVTEAAAPLPSDDPMEALKQSSAADFNAYK
                     EAEAALAAAEAAGNGAEVETLRARVTELKARSDASKAAMKEARARQKEDIQAKNAGHD
                     PLKAAKLEVAKTGVLLKKARKALEAAQANGEGDIAALTAAAQEAEAAAASAEDAVKKL
                     EGVE"
     gene            3665..>4342
                     /locus_tag="VFX11_08865"
     CDS             3665..>4342
                     /locus_tag="VFX11_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX4938772.1"
                     /translation="MALLNVTSPHVHKPGNTGAFMREVIYATVPGVAIMTYFFGWATL
                     INIALACAIAVGCEAAVLKLRHKPIAFYLRDYSAVVTALLLAIAIPPTAPWWLTLVGT
                     AFAIVVAKHLYGGLGMNPFNPAMIGYVLLLISFPKDMTTWLPAAGVQESVAALNPIQS
                     LQMIFTGHISGVDAFSGATPLDTFKTYAGQPSVLDANPILNGAMAGYGWEWVNVAFLL
                     GGIYLIWR"
BASE COUNT          919 a         1353 c         1314 g          756 t
ORIGIN      
        1 ggaagatctg gtgggcaagc tgaccgtgat ggtagccaac ctggccccac gcaagatgaa
       61 attcggcatg tcggaaggca tggtgctggc cgccggcccc ggcgggaagg acatcttcat
      121 cctggaaccg gacagcggcg ccacccccgg catgcgagtg aagtaatcgt ggtaaaattc
      181 cgctctgccg tactgtctga ctgacgcaaa gggttaccgt catgcctgag tttctgctgg
      241 ttttgatcag caccattctg gtcaacaatt tcgtgctggt gcagtttctc ggcctgtgtc
      301 cgttcatggg cgtgtccaac aagctggaaa ccgccatcgg catggcgatg gccaccacct
      361 tcgtactcac gctgtcgtcc gtgctcacct acctgaccta cacctacatc ctggtgccgc
      421 tggatgtggg ttacctgaaa accatgtcct tcatcctggt gattgccgtt accgtgcagt
      481 tcaccgaaac cgtgatgcgc aagaccagcc ccctgctcta tcgcgtgctg ggcattttcc
      541 tgccactgat caccgtgaac tgcgccgtgc tgggtgtggc cctgctcacg gtcaagaaag
      601 gcgtcgacag tttcatatac gctttcctgt atggcttcgg cggcgcactg ggcttctcgc
      661 tggtgctgat cctgttcgcc gccatgcgcg agcggattct ggtgtccgac gtgccaaccc
      721 cgttcaaggg cgccagcatc ggcatgatca ccgccgggct gatgtcgctg gcgttcatgg
      781 gcttttccgg cctgatcaag atttagggca gtcagtatgg atcttctgat caccaatatc
      841 ggagcagttt cggcactggt ggccctggcg ctggcgttcg gcgccatcct cggttatgcc
      901 gccgtgaaat tccacgtgga aggcaatccc atcgttgacc agatcgacga cctgctgccg
      961 caaacccagt gcgggcagtg tggacacccc ggctgccgtc cctacgccga aggcatggcc
     1021 gcgggcgaag agatcaacaa gtgcgttccc ggtggtgaag acacgattcg cgcgctggcg
     1081 gatctgctgg gccgtgaatt tgtgccgctg gatgcggagt acggtgagga aaaacaacac
     1141 aagatggtgg cccacatccg cgaggcggag tgcatcggct gcaccaaatg cctgcaggcc
     1201 tgcccggtgg atgcgatcct cggcgccgcc aagcagatgc acaccgtgat ggtggatgaa
     1261 tgcaccggct gtgatctgtg cgtggagccc tgtccggtgg actgtatcga catgatcccc
     1321 gtgaaaacga cccagcagac ctggacctgg cagccgcccg ccggcattgg cgcccgtccc
     1381 ggtgacctga ttccggtaag gaacattgca taatgagtat ctgcgcatga gccgtcgtat
     1441 ctgggacatt cccggcggca ttcatccacc ggaaaacaaa cagcagtctt cccgcaatgc
     1501 cattcgcccg gccgcgattc ccgcgcgtct ggtagtgccc gtgcagcagc acatcggtgc
     1561 accggccaaa ccgctggtgc aggtgggcga ccacgtactg aaaggccaga agatcggcga
     1621 ggccaccggc tttgtcagcc tgccggtgca tgcccccacc tccggcgtgg tggaggccat
     1681 cgaactacac gcggtccagc atccatcgca gattgattcg ctctgcatca ccctcaaaac
     1741 cgatggcgaa gaccgctgga ctgaacgcca gccgttagcc gattaccgcg cccataccaa
     1801 aaaggaagtg ctggaccgca tccgcaacgc cggtattgcc ggtatgggcg gcgccggttt
     1861 tcccacggtg gtgaaactgg gtgtaaaggc cgacgcgcag atcgaacagc tgattctcaa
     1921 cgcggtggaa tgcgaaccct atatcaccgc cgacgaccgc ctgatccagg aacgggccgc
     1981 ggaagtcgtt gccggtctgc agatcatggc ctggctggtg gagccgaagg aaatcctgat
     2041 cggcatcgag gacaacaagc cgcaggcaat cgaagcgctg cgcaatgtct gcgccggatt
     2101 gaccaaacaa aacggcgcga ccatcgacgt tgtagtggtg cccaccaagt atccgtccgg
     2161 cggcgaaaag cagctgatcc agattctgac cggaaaagaa gtgcgcagtg gcggcctgcc
     2221 tgcggacgtg ggcgtgatct gccagaacgt gggcacggcc tatgcggtca agcgcgcgat
     2281 catcgatggc gaaccgctga tttcccgcat caccacactg accggcgatg cgatcgaaca
     2341 gaaaggcaat tacgaagtgc tgctgggcac accggtggcg gacctgctgc aacaggccgg
     2401 cctgaaaaaa gacaagctgc accggctggt gatgggcggc cccatgatgg gctttaccat
     2461 tcacgatccg gcggtgccgg tggtgaaaac caccaactgc ctgctggtgg ccaccgaaca
     2521 ggaatttccc gaccctgcgc cggaacagcc ctgcatccgc tgtgcgtcct gcgcccaggc
     2581 ctgcccggtg aatctgctgc cgcaacagct gtactggttt gccaaagcca aggaattcga
     2641 caaggccgag cacttcaacc tgttcgactg cattgagtgt ggcgcctgtt cctacgtgtg
     2701 ccccagcagc attccgctgg tgcagtacta ccgtttcgcc aagggcgcca tccgccacca
     2761 gcagcaggaa gtgcacaagg ccgaccgcgc ccgcgaacgt ttcgaggccc gcaaggcccg
     2821 catcgagcgc gaggaacagg agaaggaact gaagcgccag gaacgcgcca aggccgccgc
     2881 tgcgaaacag tcggcgaaga aggaagcccc tgcaggcgca gcatcaacgg ccactgcgcc
     2941 cgcgccaaca agcggagcgg caccggcatc ccccgctgca actgacgcag caccggtggc
     3001 cgacgaactg accaaactgc aaaccgctgc cgcttcgacg ttgaagcgct acaaggatgc
     3061 gcagaaggcg ctggaagtgg ccgaaaagaa cggctcggaa catatcgacg ccctgcgcgc
     3121 caaagtcgaa cagctgaaaa ccaaggctga cgccgcaaaa gatgcattca tcgccgcgaa
     3181 agccgcagaa aaatccgcac cggattccgg cgtgacagaa gccgccgcgc cattgcccag
     3241 cgatgatccg atggaggcac tgaagcaatc ctccgctgcc gatttcaacg cctacaagga
     3301 agcggaagcc gcactggccg ccgccgaagc ggccggtaat ggtgccgagg tggaaacatt
     3361 gcgcgcccgt gtgacggaac tgaaagcccg gtccgacgcg tcgaaagcgg cgatgaaaga
     3421 agcccgcgcc agacagaaag aagacatcca ggccaaaaat gccggccacg acccgctgaa
     3481 agccgccaaa ctggaagtcg ccaaaaccgg cgtgctgctg aaaaaagcgc gcaaggcact
     3541 ggaagcagcg caagccaatg gtgaaggcga catcgctgca ttgacagcag ccgcacagga
     3601 agccgaagcg gccgccgcca gcgccgaaga cgccgtgaag aaactggaag gggtggagta
     3661 aaagatggcc ttgctgaacg taacctcccc ccatgtgcac aaacccggca acaccggcgc
     3721 gttcatgcgc gaagtgatct acgccaccgt gcccggcgtc gcgatcatga cgtacttttt
     3781 cggctgggcg acgctgatca atatcgcgct ggcctgcgcc attgcggtgg gctgcgaagc
     3841 ggcggtgctg aaactgcgcc acaagccgat tgcgttttac ctgcgcgact acagcgcggt
     3901 ggtcactgcc ctgctgctgg ccattgcgat tccgcccacg gcgccctggt ggctgacact
     3961 ggtgggaacc gcctttgcca tcgtcgtcgc gaaacatctg tacggcggcc tgggcatgaa
     4021 tccgttcaac ccggcaatga tcggctacgt tctgctgctg atttcctttc ccaaggacat
     4081 gaccacctgg ctgccggcgg caggcgtgca ggaaagcgtt gccgctctga atcccatcca
     4141 gtcattgcag atgattttca ccggccacat cagcggtgtt gatgcgttca gcggcgccac
     4201 gcccctcgac acattcaaaa cctacgccgg gcagccgtcc gtgctggacg ccaacccgat
     4261 cctcaacggc gcgatggccg gctatggctg ggaatgggtg aatgtcgcgt tcctgctggg
     4321 cgggatctac ctgatctggc gc
//
LOCUS       DASUEG010000475         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_201822, whole genome shotgun sequence.
ACCESSION   DASUEG010000475 DASUEG010000000
VERSION     DASUEG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201822"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFX11_08870"
     CDS             complement(<1..895)
                     /locus_tag="VFX11_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4938773.1"
                     /translation="MLQFFSHWYKQYFSDQEAVLLMVVLVVGFTILITMGSVLAPVLT
                     AIVLSYLLQGLINTLKRHRVPPLVAFLSTYIVFLASQLAFLFVLVPLTWGQLERLVNE
                     QLPRLLVEANVFLGMLPERYPELVSPDAARELSSYTTKAVTDVVQSVLSVSLANFENL
                     MGVLIFLVLVPLLVFFFLKDKDRLIAWAVSFLPEDRPFISRVMTEMDTQISNYVRGKA
                     IEILICGGVTYIALVVMGVNYAALLGLLVGLSVVVPYIGAVVVTIPVAVVAYFQFGLS
                     SEFMWVMIVYTVIQGLDGNVLV"
     gene            complement(905..1138)
                     /locus_tag="VFX11_08875"
     CDS             complement(905..1138)
                     /locus_tag="VFX11_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX4938774.1"
                     /translation="MNDIIQLDARGLQCPLPLLKLKQQLNRMLPGQQIRIMTTDPGSV
                     RDFSAFAAQAGHRLLQSGEQAGEFQFLIEKGDQ"
     gene            1349..>2351
                     /locus_tag="VFX11_08880"
     CDS             1349..>2351
                     /locus_tag="VFX11_08880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4938775.1"
                     /translation="MPLRILVWLSALIVSQLFWAAALQAEELPRLGDSTSSVVSPEQE
                     YRLGRAWLRQLRAQAPIIQDPLIHEYFYDLVYRLAANSEIQRPELETIVLNSPDINAF
                     AVPGGIIGLNGGLLLNARTEDELAGVIAHELAHLSQRHFARAMERSQNTSWAGLAALL
                     ASVVIAATAGGDAGMAALATTQAASIESQLRFSRNNEQEADRIGMQTLARSGMNPGAM
                     ADFFEALQRSMRYSGELPPEFLLTHPVTESRITDARARAAQLPATPASNSLEFGLMKM
                     RTSVMFARDTSATINDLLNQRKSGTEFLEVNEYGLACAYSKANQLDQALATINGLLLR
                     "
BASE COUNT          510 a          775 c          653 g          413 t
ORIGIN      
        1 gcaccagcac attgccatcc agaccctgga tgaccgtgta cacgatcatc acccacatga
       61 actccgaact gaggccgaac tggaagtagg ccaccaccgc caccggaatg gtcaccacaa
      121 cggcgccgat gtagggcacc accacgctca gccccaccag cagacccagc aatgctgcgt
      181 aattcacccc catcaccacc agggcaatgt aggtcacgcc accgcagatc aggatctcaa
      241 tcgccttgcc ccgcacatag ttggaaatct gcgtgtccat ctccgtcatg acacgggaaa
      301 tgaaagggcg atcctccggc aggaagctga ccgcccaggc aatcagacga tccttgtcct
      361 tcagaaagaa aaacaccagc aggggcacca gcaccagaaa gatcagcacg cccatcaggt
      421 tttcgaagtt ggccagggaa acgctgagta ccgactgcac tacgtccgtc acggccttgg
      481 tggtatagct gctgagttcc cgcgcggcat ccggcgacac cagctccggg tagcgctccg
      541 gcagcatgcc aaggaaaaca ttggcctcca ccagcaaacg cggcagctgt tcattcacca
      601 gccgctccag ctggccccag gtcagcggca ccagcacaaa cagaaacgcc agctgcgaag
      661 caagaaacac tatataagtg gatagaaagg ctaccagcgg cggcacccgg tgccgcttca
      721 gggtattgat cagtccttgc agcaggtagg acagcacaat ggccgtcagc accggcgcca
      781 gcaccgaccc catggtgatc aggatcgtga agccaacgac cagcaccacc atcagcaaca
      841 ccgcttcctg gtcggaaaag tactgcttgt accagtgact gaaaaactga agcatgccgt
      901 tgccttattg gtcgcccttc tcgatcagga actggaactc gcccgcctgt tcgccactct
      961 gcaacaaccg gtggcccgcc tgggcggcaa acgcgctgaa atcccgtacg gagccggggt
     1021 cagtggtcat tatccggatc tgctggcccg gcagcatccg gttgagctgc tgtttcagtt
     1081 tgagcagggg aagcggacac tgcaaaccgc gggcgtccag ctggatgatg tcattcatgg
     1141 ggcggagtgt ggcacttttg ctcgaaagag aagtctcact atagccaagc cgacccggtt
     1201 ttggtaccgg ccaatcccat taagtaaatg atctttttgg ctttgtgcac cgaatacgat
     1261 ctgctaccct tgattggccg gtcagcggat tttcaccctc ctccaacact tttcgatcgt
     1321 aagccatcct attcacatga tgctgcagtt gccattacga atcctcgtct ggctgagcgc
     1381 cctgattgtc agccagctat tttgggctgc cgcgctccag gcggaagaac tgccccggct
     1441 gggggactcc acatcatcgg tggtcagccc cgagcaggag taccggctgg gccgggcctg
     1501 gctgcggcaa ttgcgggccc aggcgccgat catccaggac ccgctgatcc acgagtattt
     1561 ctacgatctg gtttaccggc tggcggccaa cagcgagatc cagcggccgg aactggaaac
     1621 catcgttctg aacagccccg atatcaacgc cttcgccgtg cccggcggca tcatcgggct
     1681 gaacggcggc ctgctgctga acgcccgtac cgaggacgaa ctggccggcg ttatcgccca
     1741 cgaactggcg cacttgagcc agcgccattt tgcgcgggcc atggagcggt cgcagaacac
     1801 gtcctgggcc gggctggccg ccctgctggc cagcgtggtg attgctgcca ccgccggggg
     1861 cgacgcaggc atggccgccc tggccaccac ccaggctgcc tcgatcgaat cccagctgcg
     1921 gttcagccgc aacaatgaac aggaagcgga ccgcatcggt atgcagaccc tggcccgctc
     1981 cggcatgaac ccgggcgcca tggcggactt ctttgaagcc ctgcaacgca gcatgcgcta
     2041 ttccggtgaa ctgcctcccg aattcctgct gacccacccg gtcaccgaat cccgcatcac
     2101 cgacgcccgg gcccgggctg cgcagttgcc agccacgcct gccagcaact cactggagtt
     2161 cggactgatg aagatgcgca cctcggtcat gttcgcccgt gacacctcgg ccaccatcaa
     2221 cgacctgctc aaccagcgca aatccggtac ggaatttctg gaagtgaacg agtacggact
     2281 ggcctgcgcc tattccaagg ccaatcagct ggaccaggcg ttggcgacca tcaacggcct
     2341 gctgctgcgg c
//
LOCUS       DASUEG010000476         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1428788, whole genome shotgun sequence.
ACCESSION   DASUEG010000476 DASUEG010000000
VERSION     DASUEG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428788"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..455)
                     /locus_tag="VFX11_08885"
     CDS             complement(147..455)
                     /locus_tag="VFX11_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX4938776.1"
                     /translation="MGKIKVFLIVSFCALFAVLFGIFLIQNQHDIMVDLLIRVEPVQT
                     SVGRFALSFFMAGLGFGLLLCFGLSLMQGLELRASRREIRKLTAQLDKLRELSLKDAA
                     "
     gene            complement(547..861)
                     /gene="ihfB"
                     /locus_tag="VFX11_08890"
     CDS             complement(547..861)
                     /gene="ihfB"
                     /locus_tag="VFX11_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX4938777.1"
                     /translation="MTKSELIERVAQRQSQLSQKDVELAVKTMLEEMSQALATGGRIE
                     IRGFGSFSLHYRAPRLGRNPKTGESVDLTGKYVPHFKPGKELRDRVNGEESDGGAEPP
                     SA"
     gene            1252..2712
                     /locus_tag="VFX11_08895"
     CDS             1252..2712
                     /locus_tag="VFX11_08895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX4938778.1"
                     /translation="MLIIPADRGIDWRRAPVATGAIILLCCLVFFSWQWHDDDRLEAA
                     ATFYLQEDLLKLEYPVFISYLSQSGRKDAANRMEAIWADGDQQTVALHILSDHGFTRD
                     LEDTDADFWGEDVYFHWQEKRTQLNEMLGRISAFHMGLIPAEHRPITFLTYQFLHGDT
                     LHLIGNMVVLAIVAAGVEAAIGSFNFLLCYLVCGLFAGIFFTLINLGGYVPLVGASGS
                     ISGVLGMYAVLYGMRKIRFFYSAIFYFGYFTAPALLVLPVWILWEIINAVWGETVGIA
                     YWAHAGGLLAGGAGMALGRKYLLQVEETYLDTGPDEDEQYRKALDDFLKQLGAFNFDA
                     ARRKLADLETQYPGKPAVMEHRYYLEKLQPKSESFHQCALNLLGRAAADPWLVHIQHD
                     VFRDYMQHDGLPRISDDMLLKLMLSFSQIEAWETLREAMKEVSRRQLRHPMLVKVLRL
                     LARGVGGKGERDLSERYQSMASSMEKELKEPQPSPR"
     gene            2835..>3342
                     /locus_tag="VFX11_08900"
     CDS             2835..>3342
                     /locus_tag="VFX11_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4938779.1"
                     /translation="MIEWSEPQRMLRDALRNFIEKEIAPNLDQLEHGDTPPYDVLRKM
                     LRTFGIDQMAKQRFEQQIAREKDELAGIVREKKEKTPEEQAMADMMRGEEAAMSMLPS
                     IELCRFCPGMVTALGVSMGLTAGTIMSKGTIAQKERFVPQLLTLEKVGAWAITEPNSG
                     SDAFGSMKA"
BASE COUNT          731 a          929 c          967 g          715 t
ORIGIN      
        1 tgagcagcag gtttaggcca tgaaagtatt ccttgctgag gggtagggat tctgcaacga
       61 cgggcttcct gcgttcggct tttcccaaca gccagccgat gataatggcg acaaacagca
      121 gtatcccgaa cagcagatcc gccagatcaa gcggcatctt tgagtgacag ctccctcagt
      181 ttgtccagct gggcagtaag cttgcggatc tcccggcgcg atgcccgcag ttccagcccc
      241 tgcatcaggc tgagcccgaa acacagcaac aggccgaagc cgaggccggc catgaaaaag
      301 gacagcgcga accgccccac gctggtctgc accggttcaa cccggatcag cagatccacc
      361 atgatgtcgt gctggttctg gatcaggaag attccgaaca gcacggcaaa cagcgcgcag
      421 aaactgacga tcagaaagac tttgattttt cccattggag cgctctgact gggtgccatg
      481 tcgcaggcac aaaaaacggc atgacgccca tcatgccgtt tttatcaagg aattcaagga
      541 aaatgcttat gcggaggggg gttcggcgcc gccgtcggac tcctcgccgt tgacccggtc
      601 ccgcagttcc ttgcctggtt tgaagtgagg tacgtacttg ccggtgagat ccaccgattc
      661 gcctgtcttg ggattccgcc ccagacgcgg cgcccggtaa tgcagactga aactgccgaa
      721 gccgcgaatc tcgatgcgcc cgccggtggc cagtgcctgg gacatttcct ccagcatggt
      781 ttttaccgcc agttccacat ctttctggga aagctgtgat tgccgctggg cgacccgctc
      841 gatcaattct gacttcgtca aaaccgtact ccgcaatttg ttctgatccc cgttatcttc
      901 catttttgta gtacctccgc accacgtttc cctgaaaccg cgcttgaatg gatatcgatc
      961 atgatccggg gtgcaggcac cctggcaaga acaatcgggc ttaactcgtt gagaaaacta
     1021 tatttattaa gataactcaa gttttaaata tagcaaactc tggcgaaggg caagttcagg
     1081 atttatacaa attggcaata tccatcccat caacccgctg tttatgggtc atttgcgccc
     1141 ctgtttagaa ccattcccta taaaaatctg tttttcctac ggtttttgaa gcatcaggtg
     1201 ggaaaagttt gctttaatta aaaagctgaa aggacaaagg ggtttttcca catgctgatc
     1261 attcctgccg accggggtat cgactggcgt cgggcaccgg tggccacggg cgccatcatt
     1321 ctgctgtgct gcctggtttt cttcagctgg cagtggcatg acgatgatcg tctggaagcg
     1381 gccgccacct tctatttgca ggaagacctg ctgaagctgg aatacccggt gtttatcagt
     1441 tacctgagcc agtccggccg caaggatgcc gccaaccgga tggaagccat ctgggccgac
     1501 ggtgaccagc agacggtggc cctgcacatt ctgtcggacc acggctttac ccgggatctg
     1561 gaagacaccg acgcagattt ctggggcgag gatgtctatt tccactggca ggaaaagcgc
     1621 acgcaattga acgagatgct gggccgcatc agcgcttttc atatggggct gattccggcg
     1681 gagcaccgcc ccatcacctt tctcacctac cagtttctgc acggcgacac gttgcatctg
     1741 atcggcaata tggtggtgct ggccatcgtg gcggcgggtg tggaagcggc catcggttca
     1801 ttcaatttcc tgctgtgcta tctggtctgc ggtttgtttg ccgggatttt cttcacgctg
     1861 atcaatctgg gcggctacgt gccgctggtg ggcgcgtcgg gatcgatttc cggtgtgctg
     1921 ggcatgtacg cggttctgta tggcatgcgc aagatccgtt ttttctacag cgcgattttc
     1981 tatttcggtt atttcacggc gccggcgctg ctggtgctgc cggtgtggat tctgtgggaa
     2041 atcatcaacg cggtctgggg tgaaaccgtg ggcattgcct actgggccca tgccggcggt
     2101 ctgctggccg gtggtgcggg catggcgctg ggccggaaat atttgctgca ggtggaagaa
     2161 acctatctgg acaccgggcc cgacgaggac gagcagtacc gcaaggcgct ggatgatttt
     2221 ctgaagcagc tgggcgcatt caatttcgac gcggcccggc gcaaactggc ggatctggaa
     2281 acgcaatacc cgggcaagcc cgctgtgatg gaacaccgct attacctgga aaagctgcag
     2341 ccaaaatcgg agtcgttcca ccagtgtgca ctgaatctgc tgggccgtgc cgctgccgat
     2401 ccctggctgg tccacatcca gcacgacgtt ttccgcgatt acatgcagca cgacggcttg
     2461 ccgcgcattt ctgacgacat gctgctgaag ctgatgctga gcttcagcca gatcgaagcc
     2521 tgggagacgc tgcgcgaggc gatgaaggaa gtgagccgcc gccagctgcg gcatccgatg
     2581 ctggtgaagg tgctgcggtt gctggcccgg ggcgttggtg gcaagggcga gcgcgatctg
     2641 tcggagcgct accagagcat ggcgtcgagc atggagaagg aactgaagga accgcagcct
     2701 tcaccccgct aacccggttg accgaaacaa tcgccaccat gatcaccttt ggtcattacc
     2761 accgtgcgtt gaaccggttt aatctgactg tcaggtcata acaacaatac caacccagcc
     2821 agaggaaaat catcatgatc gaatggagcg aaccgcaacg catgctgcgg gatgccttgc
     2881 gcaatttcat cgaaaaggaa atcgccccca acctggacca gctggaacac ggcgacaccc
     2941 cgccctacga cgtgttgcga aaaatgctgc gcaccttcgg catcgaccag atggcgaaac
     3001 agcgcttcga acagcagatt gcccgggaaa aagacgaact ggccggcatt gtgcgcgaga
     3061 aaaaggaaaa aacaccggaa gaacaggcga tggcggacat gatgcgcggc gaggaagcgg
     3121 ccatgagcat gctgccatcc atcgagctgt gccggttctg ccccggcatg gtgacggcgc
     3181 tgggcgtgag catgggcctg accgccggca ccatcatgtc gaaaggcacc atcgcgcaga
     3241 aggaacgttt tgtaccgcaa ctgctgacgc tggaaaaagt cggcgcctgg gccattacgg
     3301 aacccaactc tggttccgat gcgttcggca gcatgaaagc ca
//
LOCUS       DASUEG010000477         7133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2078818, whole genome shotgun sequence.
ACCESSION   DASUEG010000477 DASUEG010000000
VERSION     DASUEG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078818"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFX11_08905"
     CDS             complement(<1..828)
                     /locus_tag="VFX11_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938780.1"
                     /translation="MPPTSQEKRTVLLLASAQALFQTSAVIVVTLSGLAGLQLAEDKS
                     LSTLPVAMMMVAAAVLMIPASLLMQRFGRRAGFLAGATLGCLAGLTAAASLWLHSFWL
                     FVLSSMLVGGYQSFAQYYRFAAADAASLAFRSRAIAWVTAGGVVAALAGTSIVKLAQG
                     IGSDPFLYAYLAMVALGLAALLVISRLQLPPPPVATAEEGGVRPLRQIMRQPAFITAL
                     TGSAAGYGVMMLVMTATPLAMLQCGQPDSASVIQWHVLGMFVPSFFTGHLIARFGVLN
                     "
     gene            complement(854..2017)
                     /locus_tag="VFX11_08910"
     CDS             complement(854..2017)
                     /locus_tag="VFX11_08910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4938781.1"
                     /translation="MAMTAVQGRCDARFESVRQAFSDLMNEPGQRGAGVSLYWRGETV
                     VDLWAGTRDKDARLPWQQNTLVNVFSTSKGVAALAVQKALDLGLLDLQKTIAYYWPEY
                     GCEGKKDVRVGWILNHRVGQPAIKTPLPDEALFDWERMTSTLAQESPWWQPGLSHGYH
                     MVTYGWLLGEVFRRAVGISLGQFLREELAQPLGLDMFLGVPETEFSRLADLSGSRDRP
                     QGGRLYLFDTVLGEPESMTSKALTNPKTLMTSSNAEAWRRMELPSANLHATAAALASL
                     YGKVACREGVVSAQALRRCQQEESVGDDPVLLTRTRFGPGFLLQQVGHPETGFGPGTQ
                     AFGHPGSGGSLAFADPEQELGFGFVMNQMGPYVMVDPRPRRLVDAVYQCLTRI"
     gene            2091..2474
                     /locus_tag="VFX11_08915"
     CDS             2091..2474
                     /locus_tag="VFX11_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX4938782.1"
                     /translation="MENGQTGIYSIQNLQRLANLLDNQFRIPFTRYRIGWDFIIGLIP
                     VAGDVLTGLASLFILIGARKYGVSGRIQLRMLVNILIDVLAGSVPVLGDILDASFKAN
                     ARNMRLLLDAIESRQNDGNLNNSKR"
     gene            2543..3397
                     /locus_tag="VFX11_08920"
     CDS             2543..3397
                     /locus_tag="VFX11_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938783.1"
                     /translation="MSALQTLVVDDSASARNVLKRLLERKGAQVDQSESGIQALDYLK
                     TKKPDIIFMDHTMPGMSGLEAIRAMRSNPDTATIPVVMYTSENDEAYKQEAMATGAVG
                     VIPKPATWNKISEVLAEVTYQRPAQADQIASSIDQQLTSLRDHLTFTMEKHIQRVGEE
                     LFQTFDQRLRQMEQGQSGPSTYPAKGLATLIHSITDSKLHQLNLELRHHITAKFDVLT
                     QDLAQSQAALKQDILQEVDQRIRVNNARRRQRQLLRIPQLLRDRWYLIPFWLAVALMG
                     GLAALWLS"
     gene            3609..4628
                     /locus_tag="VFX11_08925"
     CDS             3609..4628
                     /locus_tag="VFX11_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938784.1"
                     /translation="MAIKHALVVDDSKSARFSLKKLLQQQGIKTDFAESAGDALNYLS
                     TNNPDIIFMDHLMPGMDGFEATKAIKSNPDTRNIPIIMCTSKEGTDYGEQALALGAYA
                     ILPKPAPSATLMAILNKLDSGDSMQSASNEKPADNKSAPTQAVGISTRVVENMVRKII
                     EERIESFQKSLGDAVRDQLKQELQADIESAKQALRQELNSQADTRINQLSNANSQRIA
                     AALFEEKFEQVNRQLENHISSNLGALETAIDATRHASPELIEEVKKIAQFTAAAAAGE
                     TAKEAAQEISRAVAQDVAQGEIANARDMIAEQLAQKSAGAGSNMLGIAGVALGAIALL
                     VALFF"
     gene            complement(4853..5221)
                     /locus_tag="VFX11_08930"
     CDS             complement(4853..5221)
                     /locus_tag="VFX11_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX4938785.1"
                     /translation="MQEQGQLPFETSGPVSVRPAVALNLLREHATYLLQVRGDAMQSA
                     GIHDGDLIAVRKSVRAGEGKLVVARVVDAAAPTPRWRGRQADATLSERTASLPFILAP
                     DNLVINGRFVGVVRERRTLH"
     gene            5452..6513
                     /gene="dinB"
                     /locus_tag="VFX11_08935"
     CDS             5452..6513
                     /gene="dinB"
                     /locus_tag="VFX11_08935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX4938786.1"
                     /translation="MTRKIIHIDCDCFYAAVEMRDDPSLRNRPVAVGGDPGRRGVIST
                     CNYEARRYGVRSAMASAYAMRLCPELIILPHRIRYYSEVSQQVMRVLRQYTDRIEPLS
                     LDEAFLDVTDQSHFQGSATRMAEAIRKQVRAQVGITVSAGVAPNKFLAKIASEWKKPD
                     GLFVIPPQDVDAFVQNLPVEKLHGVGKVTAERLHRLGFRTCADIRACNHFQFIEKVGS
                     FGEYLYQLAHGRDERPVQERDERKSLSVEHTYAQDLPDLGSCIRQLPALKERLESRLS
                     RSRQKDEVKKAFVKIKFADFTSTTMECCVQEPTLPVYESLCDEAFQRKGISVRLLGVG
                     VRFNERSHSPFTQFALNFG"
     gene            complement(6510..>7133)
                     /locus_tag="VFX11_08940"
     CDS             complement(6510..>7133)
                     /locus_tag="VFX11_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938787.1"
                     /translation="YNSGLPIWENGVALARLLEEQWSPAAPGSLVLVGHSMGGLLIRS
                     ALHQAGDAGWTRAVTHAAYLASPHAGAPLERIGNFANSLLGVSPYSKPLMALGNIRSL
                     GIRSLRHGNVTPPQDASENQVPLPLQDGVHHLLLAASFVDDPSKRWLGDGLVPVQSAL
                     GESHFPVGQGQVERVFIDNMGHMKLLQDERTYTTLRNWLRSSSSATV"
BASE COUNT         1588 a         2292 c         1992 g         1261 t
ORIGIN      
        1 gttcaacaca ccaaaccgcg caatcaaatg cccggtaaaa aacgacggca cgaacatgcc
       61 cagcacatgc cactgaatca cactggcgga atccggctgt ccgcactgca acatggccag
      121 cggagtggcc gtcatcacca gcatcatcac gccatagccg gccgcagacc cggtcaacgc
      181 ggtaatgaaa gccggctgac gcatgatctg ccgcaaggga cggacaccac cctcctccgc
      241 cgtggccacc ggaggcggcg gcagttgcag gcgggaaatg accagcaggg ccgcaagccc
      301 cagcgccacc atggccagat acgcgtacag gaacggatcg ctgccgatcc cctgcgccag
      361 cttgacgatg ctggtaccgg ccagtgcagc caccactccg cccgccgtca cccaggcgat
      421 ggcgcggctc ctgaacgcca ggctggccgc gtccgccgcc gcaaaccggt agtactgggc
      481 aaacgactga tacccgccca ccagcatgct ggacaacacg aacagccaga agctgtgcag
      541 ccagagtgat gccgccgcgg tcagccccgc gaggcagccc agcgtcgcgc cggccagaaa
      601 cccggcccga cggccgaacc gctgcatcag caacgaggca ggaatcatca acactgctgc
      661 cgccaccatc atcatggcca ccggcaacgt cgacagactc ttgtcctcgg ccagctgcag
      721 ccctgccaat ccggaaaggg tcaccacaat cacggcggag gtctgaaaca aagcctgggc
      781 agaggccagc agcaggaccg tacgtttttc ctgggaagtg gggggcaagt gaattccttg
      841 tagtgcggat cgttcagatc cgcgtcagac actggtaaac cgcatccacc agccggcgcg
      901 ggcgtgggtc caccatcaca taaggcccca tctggttcat cacgaaacca aaacccaatt
      961 cctgctcagg atcggcaaac gccagcgagc cgccggaacc aggatggccg aacgcctggg
     1021 tgccggggcc gaagccggtc tcgggatgtc ccacctgctg cagcaaaaaa ccgggcccga
     1081 accgggtgcg ggtgagcagc accggatcat cccccacgct ttcttcctgc tgacagcggc
     1141 gcaatgcctg tgccgacacc acaccttcac ggcaggccac cttgccatac aggctcgcca
     1201 gtgccgccgc cgtggcgtgc aggttggcag acggcagctc catccggcgc caggcttcag
     1261 cattggaact ggtcatgagg gtcttgggat tggtcagcgc tttggatgtc atggattccg
     1321 gctcacccag caccgtgtcg aacaggtaca gccggccacc ctgcggccgg tcgcgggaac
     1381 ccgacaggtc cgcgagcctt gaaaattctg tttcaggcac gcccagaaac atatccagcc
     1441 ccagcggctg cgccagctcc tcccgcagaa actgccccag ggaaataccc accgcccggc
     1501 ggaacacctc ccccagcagc cagccatagg tgaccatgtg ataaccgtgg ctcagtcccg
     1561 gctgccacca gggactttcc tgcgccaggg tggatgtcat ccgctcccag tcaaacagcg
     1621 cctcatccgg cagcggcgtt ttgatcgccg gctggcccac gcggtgattc agaatccagc
     1681 ccacccggac atccttcttg ccctcgcagc cgtattccgg ccagtaatag gcaatggttt
     1741 tctgcagatc cagcaaccca aggtccaacg ctttctgcac ggccagcgcg gccactccct
     1801 tgctggtgga aaacacgttc accagggtgt tctgttgcca gggcagacgg gcatccttgt
     1861 cgcgggtgcc cgcccacaga tccaccactg tctcgccacg ccagtacagg ctgaccccgg
     1921 cgccacgctg gcccggttcg ttcatgagat cggaaaacgc ctggcgcacg gattcgaaac
     1981 gcgcgtcgca gcggccctgt actgccgtca ttgccatgca aacacctgat tagaaaagcc
     2041 caaagacccg atggtagaat ccccagcacc cccaggtaaa ggaaactact gtggaaaacg
     2101 gccaaaccgg catctattcg atccagaacc tgcaacgact ggccaatctg ctcgacaacc
     2161 agttccgcat ccccttcacc cgctaccgca tcggctggga tttcattatc ggcctgattc
     2221 cggtggccgg tgacgtgctg accggtctgg cctcgctgtt catcctgatt ggcgcgcgca
     2281 aatacggcgt atccgggcgg atccagctgc gcatgctggt gaatatcctg atcgacgtgc
     2341 tggcaggatc ggtgcccgtt ctgggtgaca tcctggacgc cagcttcaaa gccaacgccc
     2401 gcaatatgcg attgctgctg gatgccatcg aatcccggca aaatgacggc aacttgaaca
     2461 attcaaagcg atagacatac tgaatacaag tgcagtaaat tggcccttcg ccttcataac
     2521 aaaaaataac ggcggttatt ccatgtcagc actgcaaact ctcgtggtcg acgactccgc
     2581 ctcggcgcgg aatgtgctca aacgtctgct ggagcgcaag ggcgcccagg tggaccagag
     2641 cgaatccggc atccaggcgc tggattacct caagaccaaa aagcccgaca tcatcttcat
     2701 ggatcacacc atgcccggca tgagcggcct ggaagcgatc cgtgccatgc gcagcaatcc
     2761 cgacaccgcc accatcccgg tggtgatgta caccagcgaa aacgacgaag cctacaagca
     2821 ggaagccatg gccaccggcg ccgtgggtgt gatccccaag cctgccacct ggaacaagat
     2881 ttccgaggtg ctggcggaag tcacctacca gcgtcccgcc caggcggacc agatcgccag
     2941 cagcatcgac cagcagctga cctcactgcg ggaccacctc accttcacca tggaaaagca
     3001 catccagcgg gtcggtgaag agctgttcca gacctttgac cagcgcctgc ggcagatgga
     3061 acagggccag tccggcccgt ccacctaccc ggccaaaggc ctggccaccc tgatccactc
     3121 catcacggat tccaagctgc accagctcaa cctggaactg cgccaccaca tcaccgccaa
     3181 gttcgacgtc ctgacgcagg atctggccca gagccaagca gcactgaagc aggacatcct
     3241 gcaggaagtg gatcagcgca tccgggtcaa taatgcccgc cgccgtcagc gccagctgct
     3301 gcgcatcccc cagttactgc gggaccgctg gtacctgatc ccgttctggc tggcagtagc
     3361 gctcatgggc gggctggcgg cactctggct tagttgacgg atcagcagaa acagtctgac
     3421 ttcccatgaa ccggtccctg catcctgtcc gcctggcgaa taggcctcgg caaagtcggc
     3481 caaagtttgt catacttaac aagcgcttaa tgtgcccaaa tccggcggtt tgacggctat
     3541 cgcggccgta atgcggtcta tactgtcaat tccgacacat ctgatcgagt actacagaag
     3601 aagtgcccat ggctatcaaa catgctctgg tggtggatga ctccaagtcc gcccggtttt
     3661 cattgaagaa attgctccag caacaaggca tcaaaacgga ctttgccgag tcggcaggcg
     3721 atgcgctgaa ctacctgtcc accaacaacc ccgacatcat tttcatggac catctcatgc
     3781 cgggcatgga cgggttcgaa gccaccaagg ccatcaaatc caatccggac acccgcaaca
     3841 tcccgatcat catgtgcacc tcgaaggaag gcaccgacta cggcgaacag gcgctggccc
     3901 tgggcgccta tgccattctg ccgaaacccg ctccgtccgc caccctgatg gccatcctca
     3961 acaaactcga ttccggtgac agtatgcagt ccgcgtcgaa cgaaaaacct gctgacaaca
     4021 aatccgcccc cacccaggcc gtgggcatca gcacccgtgt agtggaaaac atggtgcgca
     4081 agattatcga agagcgcatc gaatcatttc agaaatctct cggcgacgct gttcgcgatc
     4141 agctgaaaca ggaactgcag gcagacatcg aatccgccaa gcaggcgctg cggcaggaac
     4201 tcaattcgca ggccgatacc cgcatcaacc agctgagcaa cgccaacagc cagcgcatcg
     4261 ccgctgcgtt gttcgaggag aaattcgagc aggtcaatcg gcagctggaa aaccacatct
     4321 ccagcaatct gggggcactg gaaaccgcca tcgacgcgac ccgtcacgcc tcgccggaac
     4381 tgatcgagga agtgaaaaaa atcgcccagt tcaccgcagc tgcggccgcc ggtgaaaccg
     4441 ccaaggaagc cgcccaggaa atcagccgcg ccgtggccca ggatgtagca caaggcgaaa
     4501 ttgccaacgc acgcgacatg atcgccgaac agctggcaca gaaatccgcc ggtgccggca
     4561 gcaacatgct ggggatcgcc ggcgtggcgc tgggcgccat tgccctgctg gtagccctgt
     4621 tcttctgatc cgtatatttg ccggcggaga tggcgatttt gttacaccaa aaccgccggc
     4681 cttcgttctt cgaacaaaaa gcttcgaata aaaaactgtg aataaaaaaa ggctgcggga
     4741 ttaaccgcag ccaaacgagg cagtaaacgt tgggtgacga ttttactgcg cgagaagtct
     4801 tttatttgtc agcgttacgc gaataatctt cctccgatat ctcccggcca gatcagtgca
     4861 gcgttctgcg ctcccgcacc acgccgacaa accgcccgtt gatgaccaga ttgtccggcg
     4921 ccaggataaa cggcaggctg gccgttcgtt cggacagggt cgcatccgcc tggcggcctc
     4981 gccagcgggg tgtcggtgcc gctgcatcca ctacccgtgc caccaccagc ttgccctccc
     5041 cggcccgcac ggatttgcgc accgcgatca ggtctccgtc gtggatgccg gccgactgca
     5101 tggcatcacc ccgcacctgc aacagatagg tcgcatgttc ccgcaacagg ttgagcgcca
     5161 ccgcaggccg caccgataca ggtccgctcg tttcaaaggg cagttgacct tgttcctgca
     5221 tgaccggatt cctcctgacg gcgtttttat atatcgcgag caagactgat gaaaaaacaa
     5281 ggcagaacgt gcaattcacc agcctgcccg gttttgaatt catccggtaa tcatgttcag
     5341 gccgcatccc gatatttagc gccgctgctt ttcatgacca ccatttactg tatataatta
     5401 cagtaaatca acatcgcagt tttggcaaag gctttttcac gaaaatttcc catgacgcga
     5461 aaaatcattc atatcgattg cgactgtttc tatgcagcgg tggagatgcg cgatgatccg
     5521 tctttgcgta accggcccgt ggcggtgggt ggcgatcccg gccggcgcgg cgtgatctcc
     5581 acctgcaatt acgaggcacg gcgctacggc gtgcgctcgg cgatggcgtc ggcctatgcg
     5641 atgcggctgt gtcccgagct gatcatcctg ccgcatcgca tccgttatta cagcgaggtg
     5701 tcgcaacagg tgatgcgggt actgcgccag tacactgacc ggatcgaacc gctgtcactg
     5761 gatgaagcat ttctggatgt aacggatcaa tcgcattttc agggcagcgc cacccgcatg
     5821 gcggaagcga tccgcaagca ggtgcgcgcg caggtgggca ttaccgtatc cgccggtgtg
     5881 gcgcccaaca aattcctggc gaagattgcc agcgaatgga aaaagccgga cggcctgttc
     5941 gtgattccac cgcaggatgt ggatgcgttt gtgcagaacc tgccagtgga aaaactgcac
     6001 ggcgtgggca aggtgacggc ggagcggttg catcggctgg gctttcgcac ctgcgccgat
     6061 atccgcgcct gcaatcattt tcagttcatc gagaaagtag gcagtttcgg cgagtatctg
     6121 tatcagctgg cccatggtcg cgatgagcgt ccggtgcagg agcgggacga acgcaaatca
     6181 ctgagtgtag agcacacgta tgcgcaggat ctgccggatc tgggcagttg tatccgccag
     6241 ttgccggcat tgaaggaacg cctggagagc cggctcagcc gctcgcgcca gaaggatgaa
     6301 gtgaagaaag cgtttgtgaa aataaagttc gcggatttca ccagcacgac gatggaatgt
     6361 tgcgtgcagg aacctacgct gccggtgtac gaatccctct gcgacgaagc gtttcagcgc
     6421 aagggcatct cggtgcgcct gctgggagtg ggggtgcgct tcaatgagcg gtcgcactcc
     6481 ccgtttaccc agtttgcact gaatttcggc tagaccgttg cactggacga cgaccgcaac
     6541 cagttccgca acgtggtata ggtgcgttcg tcctgcaaca gcttcatatg gcccatgttg
     6601 tcgatgaaga cacgctcgac ctgtccctgc ccgacgggaa aatgactttc ccccaacgcg
     6661 ctttgcaccg gcaccagacc atcgcccagc cagcgcttgg acggatcgtc cacaaaactg
     6721 gcggccagca gcagatgatg aacgccgtcc tgcaacggca acggcacctg gttttcactg
     6781 gcgtcctgtg gcggtgtcac attaccgtgc cgcaggctgc gaatgcccag gctgcgaata
     6841 ttgcccagcg ccatcagcgg tttcgaatag ggggaaacac ccagcaggga attggcgaaa
     6901 ttgccgatcc gctccagggg cgcgccggca tggggggacg ccagataggc agcatgcgtc
     6961 acggcgcggg tccagcccgc gtctccagcc tgatgcaacg cactacgaat cagcaggccg
     7021 cccatgctgt gtcccaccag caccagcgat cctggtgccg ctggcgacca ttgctcctcc
     7081 agcaggcgcg ccagggcgac gccgttctcc cagatcggca ggccactgtt gta
//
LOCUS       DASUEG010000478         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_324625, whole genome shotgun sequence.
ACCESSION   DASUEG010000478 DASUEG010000000
VERSION     DASUEG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324625"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFX11_08945"
     CDS             complement(<1..211)
                     /locus_tag="VFX11_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEX4938788.1"
                     /translation="MTLNRIDDALVQHWLIPDWSAPDRVPAHVRGYVTTRAGHLSAHP
                     YDGFNTANHVGDDPGHEEANRQSLAE"
     gene            complement(192..1148)
                     /gene="rluD"
                     /locus_tag="VFX11_08950"
     CDS             complement(192..1148)
                     /gene="rluD"
                     /locus_tag="VFX11_08950"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003258205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEX4938789.1"
                     /translation="MTQHIQLQAEVPDSLAGQRLDQAAAELFPDYSRSRLQTWIKNGD
                     LKVNGEIRKTREKVLGGETLELSAELVADESWEPQPIDLDIVYEDEHLLVINKPVGLV
                     VHPGAGNTDSTLLNALLHHCPELATLPRAGIVHRIDKDTTGLLVVAKTLKAHTSLVDQ
                     LQEKTAYREYEAICTGVMTGGGKVDEPIGRHPTIRTKQAVTHSGKPAVTHYRVLERFR
                     GHTYIKVQLETGRTHQIRVHMAHIHYPLLGDPVYGQRLRLPKGATPELIDAMRRFKRQ
                     ALHARKLGVQHPGTGEYMEWETPLPDDFKTMLQVLRHDAQSD"
     gene            1281..2117
                     /locus_tag="VFX11_08955"
     CDS             1281..2117
                     /locus_tag="VFX11_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX4938790.1"
                     /translation="MRIRLLPFLVATFLLATALLTGCASDPKTPQLSERQFYEEAQDA
                     IENNNMTMAIERLQGLESRYPFGRYASQAKLDLIYCHYRALDYEAAAATAERFINTNP
                     DHAQLDYAYYMKGLSSYSVDRGLIERFIPSDFTERDMGPARESFEDFNRLINRFPNSV
                     YAADARQRMIYLRNLLAAYELKAADYYMRRGAYVAAANRGRYVVENFDSSPSVPDALA
                     IMAKAYAELGLEDLKTSTVAVLKHNYPRHPDLDASGNLVYETGKRGKQSMLNVVSFGL
                     LD"
     gene            2162..2584
                     /locus_tag="VFX11_08960"
     CDS             2162..2584
                     /locus_tag="VFX11_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HEX4938791.1"
                     /translation="METVMDKTVADARTVVEEFLQASSELDMDAALALIADDCVYQNV
                     PFHTARGKRNVAKALQGMGKAMTEFKVDMINIASNGDVVMTERVDTLGGKFFRAELPV
                     MGVFVVKNGKITEWRDYFDWSLSFGKFIGSVFTTPFRK"
BASE COUNT          482 a          745 c          838 g          546 t
ORIGIN      
        1 attccgccaa ggattgccgg ttggcttcct catgaccggg atcatcgccc acgtgattgg
       61 cggtattgaa gccgtcatag ggatgggcgc tgagatggcc tgcgcgggtg gtgacatagc
      121 ctcttacatg ggccggcaca cgatccggtg cggaccagtc ggggatcagc cagtgttgga
      181 ccagtgcgtc gtcaatccga ttgagcgtca tggcgcagta cctgcagcat ggttttgaag
      241 tcgtccggca gcggagtttc ccattccatg tactccccgg tgcccgggtg ttgcacgccc
      301 agcttgcggg catgcagggc ctggcgcttg aagcggcgca tggcgtcgat cagttccggc
      361 gtggcgccct tgggcagacg caggcgctgg ccgtatacgg gatcgcccag cagcgggtaa
      421 tggatatggg ccatgtgcac gcggatctgg tgcgtacggc cggtttccag ttgcaccttg
      481 atgtaagtat ggccgcgaaa ccgttccagc acacggtaat gggtcaccgc tggcttgcca
      541 ctgtgggtga cggcctgttt ggtgcggatg gtgggatggc ggccgatggg ctcatccacc
      601 ttgccgccgc cggtcatgac gccggtgcaa atggcttcgt attcgcggta agccgtcttt
      661 tcctgcaact ggtccaccag ggatgtgtgc gctttcaggg tcttggccac caccagcagg
      721 cccgtggtgt ccttgtcgat gcggtggaca atgccggccc ggggcagcgt ggccagctcg
      781 gggcaatggt gcagcaaggc gttgagcagc gtgctgtcgg tattgccggc gcccgggtgc
      841 accaccagtc ccaccggctt gttgatcacc agcaggtgtt cgtcctcgta gacgatgtcc
      901 agatcgatgg gctggggttc ccagctttcg tccgccacca gttccgccga cagttccagg
      961 gtttcacccc ccagcacctt ttcccgggtt ttgcggattt caccgttcac cttgaggtcg
     1021 ccgttcttga tccaggtttg caggcgggag cgggaataat cgggaaacag ctcggccgcg
     1081 gcctggtcca gacgctgtcc ggccagggaa tcgggcactt cggcctgcag ttgaatatgt
     1141 tgggtcatgg aactcgctcg gctgggatcg gtctgccatt ctagcaagtg cggcgaccaa
     1201 tgtggtcatt acagtgattg ccgttataat gcccggctac cgatttccct tttccgacca
     1261 gaatccgagt gacttcccgc atgcgaatcc ggcttttgcc tttccttgtt gcgactttcc
     1321 tgctggccac cgccctgctc accggctgtg cgtccgatcc gaaaacgccg cagctgtccg
     1381 agcgccagtt ctatgaagag gcgcaggatg ccatcgaaaa caacaacatg acgatggcga
     1441 tcgagcggct gcagggtctg gaatcgcgct atccgttcgg ccgctatgcg tcgcaggcga
     1501 agctggacct gatctactgc cattaccggg cgctggatta cgaggccgct gccgccaccg
     1561 ccgaacgttt catcaacacc aaccccgatc atgcccagct ggactatgcc tactacatga
     1621 agggtctgtc gtcgtacagc gtggaccggg gcctgatcga gcgcttcatt cccagcgatt
     1681 tcaccgagcg ggacatgggg ccggcgcggg aatcgttcga ggatttcaac cgcctgatca
     1741 accgcttccc caacagtgtt tacgcggcgg atgcacgtca gcgcatgatt tacctgcgca
     1801 acctgctggc ggcctatgaa ctgaaggcgg cggattacta catgcggcgc ggcgcctatg
     1861 tggcggcagc gaatcgtggc cgttatgtgg tggagaattt cgattccagc ccgtcggtgc
     1921 cggatgcgct ggcgatcatg gcaaaggcgt atgcggagct gggcctggaa gatctgaaaa
     1981 cctcgacggt ggcagtgctg aagcacaact atccacgcca tccggatctg gatgcgagcg
     2041 ggaatctggt ttatgaaacc ggcaagcgcg gaaagcagtc gatgctgaat gtggtgtcgt
     2101 tcggactgct ggattaaggt gtgaacggat caacgcgcga cagaactcaa ggagaggcag
     2161 gatggaaact gtaatggaca agaccgttgc cgatgcccgg accgtggtcg aagaatttct
     2221 gcaggccagt agcgaactgg acatggacgc cgcactggca ctgattgccg acgactgcgt
     2281 gtaccagaac gtgcctttcc acaccgcccg cggcaagcgc aacgtcgcca aggcgttgca
     2341 gggcatgggc aaggcgatga ccgagttcaa ggtggacatg atcaatatcg ccagcaacgg
     2401 cgatgtggtc atgaccgagc gggtagacac gctgggcggc aagtttttcc gcgcagaact
     2461 gcccgtgatg ggcgtgtttg tggtgaagaa tggcaagatc accgagtggc gggactactt
     2521 cgactggtcg ctgtcattcg gcaagttcat tggcagcgtg ttcaccacac cgtttcgcaa
     2581 gtgaccccct ggcgtcagct gggccgcccg a
//
LOCUS       DASUEG010000479         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_553199, whole genome shotgun sequence.
ACCESSION   DASUEG010000479 DASUEG010000000
VERSION     DASUEG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553199"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFX11_08965"
     CDS             complement(<1..382)
                     /locus_tag="VFX11_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4938792.1"
                     /translation="MITGLRRLVLLIAILLCALPAQSEPVRLDVGDSMRRVSRNAHVL
                     VDQQGRLSFDDIRQAPADRWQPVATAFPNFGFTNAAVWLRLELVNTAATQRDYVLQVE
                     YPLLDSVQFHSPDPAGQYRHIETGD"
     gene            550..1104
                     /locus_tag="VFX11_08970"
     CDS             550..1104
                     /locus_tag="VFX11_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX4938793.1"
                     /translation="MKFKLLAGAALLGCSSLALAQPYIGASFGKVDYDEEGVSKPTGF
                     DLYAGFKVNEYFALEGVYTDLGNADIHFQEQYYYYDGYYDYLVTETYMGELSVDSIGF
                     AGKVIIPVGDVLELYARGGFHAWDAEISGAGGSASEDGTDPFLGFGLTFKVGNSVGLG
                     ARYTRYDLEDDDVSMTAANIELYF"
     gene            complement(1231..1881)
                     /gene="coq7"
                     /locus_tag="VFX11_08975"
     CDS             complement(1231..1881)
                     /gene="coq7"
                     /locus_tag="VFX11_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX4938794.1"
                     /translation="MASRERHYSLFDRLIGQTDNALRTVARGHQGTGRASPAIPTDIP
                     SLNEAERRHVAGLMRVNHTGEVCAQALYQGQALTARLPRIRRQMEISAREEEDHLAWC
                     EQRLDELHSHTSFLNPLWYLMSFGIGAGAGIVGDRWSLGFVEETENQVCAHLQGHLEK
                     LPGHDERTRAILQQMHTDEADHARKARRAGAMQLPGPVRKAMTLVAKVMTGTAYRI"
BASE COUNT          370 a          604 c          675 g          443 t
ORIGIN      
        1 ggtcgccggt ttcgatgtgg cggtactggc cggcgggatc gggggaatgg aattgcacgc
       61 tgtccagcag cggatactcc acctgcagaa cgtaatcgcg ctgggtggcg gcggtattga
      121 ccagctccag gcgcagccac acggcggcgt tggtgaagcc gaaattggga aacgccgtcg
      181 ccaccggttg ccagcgatcg gcaggcgcct ggcggatatc gtcgaaactg agccgcccct
      241 gctggtcgac gagaacgtgg gcgttgcggg atacccggcg catgctgtcg ccaacgtcca
      301 ggcgcaccgg ctccgactgc gcgggcagcg cgcacagcag gatcgcgatc aacagtacaa
      361 gacggcgcag gccggtgatc atggagtggt tgccagctgg ggtaatggaa tcagtatagg
      421 ttaaaccggc cagtttctgg atgggggtgt ttcacttggg cgcctgatcc cctatattcc
      481 cgtcaccgcg gggtgaaccg cactggtacc cggcggaatt cttatttttc tataaactgg
      541 agttgaaata tgaagtttaa gttactggct ggcgcagcct tgctgggctg cagttccctc
      601 gcgctggcgc aaccgtacat tggggcaagc ttcggcaagg tcgattacga cgaggaaggg
      661 gtttccaaac ctacgggatt cgatctgtat gcaggattca aggtcaatga atatttcgca
      721 ttggaaggtg tgtataccga cctgggcaat gcggacatcc attttcagga acagtattac
      781 tactacgatg gttactacga ctaccttgtc actgaaacct acatgggtga gttgagcgtg
      841 gattccatcg gtttcgcggg caaggtcatc attccggttg gcgacgtgct ggagctctat
      901 gcccgtggcg gcttccacgc gtgggatgct gaaatcagcg gcgccggcgg ttctgcaagc
      961 gaagacggca ccgatccttt cctgggcttc ggcctgacct tcaaggtggg gaattcggtg
     1021 ggcctgggtg cccgctatac ccgctacgat ctggaagacg acgatgtgtc gatgacagct
     1081 gccaacatcg aactgtactt ctgatcggtg catttggtct gtctgcagcg gctgccccgg
     1141 ttggggtggc cgcttgtttt tagccgagat tagccgagaa tttttctcct cacgtttttc
     1201 cttgctgcgg cagtcgtgtt ctgccggccc tcagatccgg tacgctgtcc ccgtcatcac
     1261 cttcgccacc agggtcatgg ccttgcgcac cggccccggc aactgcatgg cgccggcgcg
     1321 gcgggccttg cgggcgtgat ccgcttcatc ggtgtgcatc tgctgcagga tggcgcgggt
     1381 gcgttcatcg tgcccgggca gcttttccag atggccctgc agatgggcgc agacctggtt
     1441 ttcggtttct tccacaaagc ccaggctcca gcgatcgccg acaatgccgg caccggcacc
     1501 gatgccgaac gacatcaggt accagagcgg attcagaaag ctggtgtggc tgtgcagctc
     1561 gtccaggcgc tgttcgcacc aggccagatg gtcttcctct tcgcgggcgg agatttccat
     1621 ctggcggcgg atgcggggca ggcgggcggt aagggcctgg ccctggtaca gcgcctgggc
     1681 gcagacttcg ccggtgtggt tcactcgcat caggcccgcc acgtggcggc gttcggcttc
     1741 gttcagggag ggaatgtcag tgggaatagc gggggacgcc cggccggtgc cctgatgacc
     1801 gcgggccacc gtacgcagcg cgttgtcggt ctggccgatc aggcggtcga acagactgta
     1861 gtgacgttct ctgcttgcca ttgctgcgct ctccccaaac aatactgcat gacggctacc
     1921 attatagggg cagcacgccg ggcgtcacca gacgcggatc aaggctgctc cgacacccta
     1981 caccgacaca aggaatgctc atgcaatcga atcgcgcgac ccctcgtacg ccccgtcgaa
     2041 ttacgcgttc actgggcgcg ggcatcgctg ccgccattct gctggccggc tg
//
LOCUS       DASUEG010000480         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_572618, whole genome shotgun sequence.
ACCESSION   DASUEG010000480 DASUEG010000000
VERSION     DASUEG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572618"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFX11_08980"
     CDS             <1..533
                     /locus_tag="VFX11_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938795.1"
                     /translation="LVGTVSRSGFVPGEGAGFVLLMTPAAAQRAGLHPLGMITGTAAE
                     WENKLIKTQEINLGEGLIRAVNGAVSAVLPPHDTIHSIYCDINGERYRGEEWGFVCLK
                     LAHYFADPTGYCSPADCWGDMGAASGGLLLALATQAAQRGYAAGSRSLMWTGSEHGLR
                     AAAVLDAPVIPSPYRG"
     gene            541..1458
                     /locus_tag="VFX11_08985"
     CDS             541..1458
                     /locus_tag="VFX11_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HEX4938796.1"
                     /translation="MASVAIHPPKTPVTKGSMGTAKATIPNVCKMPGPPAPFVPSPLP
                     NIAKSGMSPKGYSTSVKIEGNTVAIRGASFASMGDMASKATGGGLLSANTHGPAKFIT
                     PGSMTVKIQGKAVHLLSEPMLNNCGAAGSPPNTGATMTGLGQTSTSVLDAQMELLCMI
                     FCQCLQTRPFAKQGTNVGVDRSVFETPEKIQTKTNRFDNQKCVERELEGNSTLLAEQP
                     FDMRRSPPTAIAERVRGHRRPDVVCLAAPGPAAGLNITRIVEMKFPGDAWNDGQQRAY
                     ERIAQENNPDATVEMIDGENEPCICEKTQ"
     gene            1434..>2206
                     /locus_tag="VFX11_08990"
     CDS             1434..>2206
                     /locus_tag="VFX11_08990"
                     /inference="COORDINATES: protein motif:HMM:NF023304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HEX4938797.1"
                     /translation="MHMRKDPVVLPAALEKQLPVRDAKGNAVLKIGFFLTLYFHNGHN
                     ESKRQAVLACAQDFLQLFGSQMQWGLLGVGQPKPVDQFAPDATCRYLESVDADGDDGW
                     QIYWHSDAPADASDYGFQASGTGRHDSEALGHLSCLTMHCRLDNAVCGPAELLNLAAG
                     WSQRLQPVHGYAGVGIIRAADRFVAAHCETQEYALAQRFPTLEVDYPLKHALWTREGI
                     KGGNWLTILSDAMVQQLEKISGSLALPASLSRHPYAGGL"
BASE COUNT          452 a          641 c          678 g          435 t
ORIGIN      
        1 agctggtggg tacggtttcc cgctccggat ttgtgccggg tgagggcgct ggcttcgttt
       61 tgctgatgac gcctgctgcc gcacaacgcg ccggattgca tcccctgggc atgatcaccg
      121 gcacggcggc ggagtgggaa aacaagctga tcaaaaccca ggaaatcaat ctgggcgaag
      181 ggttgatccg ggcggtgaac ggcgcggtat ccgctgtgct gccgccacac gacaccatcc
      241 actcgatcta ttgcgacatc aacggcgaac gttatcgcgg cgaggagtgg ggtttcgtct
      301 gcctgaaact ggcacattat tttgccgatc ccaccggcta ctgcagtccg gccgattgtt
      361 ggggcgacat gggcgctgcc tcgggcggat tgttgttggc gctggccacg caagccgcgc
      421 agcgcggtta tgccgcaggc agccgcagtc tgatgtggac aggatcggag catggcctgc
      481 gtgctgcagc cgtgctcgac gcccccgtca ttcccagtcc ctatcgcggg tagctgcatc
      541 atggccagcg ttgcaattca tccgccgaaa acacctgtta ccaaaggcag catgggcacc
      601 gccaaggcca ccattcccaa cgtctgtaaa atgccggggc cgccagcgcc cttcgtgcca
      661 tcgccgttgc ccaatatcgc caaatccggc atgtcgccca agggctattc caccagcgtg
      721 aaaatcgaag gcaacaccgt tgccattcgc ggcgccagtt ttgccagcat gggggatatg
      781 gccagcaagg ccaccggtgg aggattgctg tcggccaata cccacgggcc ggccaagttc
      841 atcacgccgg gttccatgac cgtgaaaatc cagggcaagg cggtgcatct gctgagtgag
      901 ccgatgctga acaattgtgg cgctgcgggc tcgccaccga atactggcgc tacgatgact
      961 gggctggggc agacgtccac cagtgtcctg gatgcgcaga tggaattgct gtgcatgatt
     1021 ttttgtcagt gtctgcagac ccgtcccttt gccaaacagg gcaccaatgt gggcgtggac
     1081 cggtcggtgt ttgaaacacc tgaaaaaatt cagaccaaaa ccaatcgctt tgataaccag
     1141 aagtgtgtcg aacgcgagct ggaaggcaat tccactttgc tggcggagca accgttcgat
     1201 atgcgtcgct caccgccaac cgccattgcc gagcgggtcc gtgggcaccg tcgacccgat
     1261 gtggtttgcc tggccgcacc ggggcccgct gccggtttga acattacccg tatcgtggaa
     1321 atgaaatttc ctggcgatgc ctggaacgat gggcagcagc gggcttatga aagaattgcg
     1381 caggagaaca accccgatgc gacggtcgag atgatcgatg gagaaaacga accatgcata
     1441 tgcgaaaaga cccagtagtg ctgccggccg cgcttgagaa acaacttccc gtcagggacg
     1501 cgaagggcaa tgccgtgctg aaaatcggct ttttcctcac cctttatttc cataacggac
     1561 ataacgaaag caaacgccag gctgtgctgg cctgcgcgca ggattttctg caactgttcg
     1621 gtagccagat gcagtgggga ttgctggggg taggccagcc aaagccggtg gatcagtttg
     1681 cgcccgacgc aacctgtcgc tatctggaat cagtcgatgc tgacggagac gacggctggc
     1741 agatttactg gcacagtgac gcgcccgccg atgccagcga ctacggattc caggcttccg
     1801 gaaccgggcg tcacgacagc gaagcattgg ggcacctttc ctgtctgacc atgcactgtc
     1861 gactggacaa tgccgtgtgc ggccccgctg aattgctcaa tctggcggca ggctggagcc
     1921 agcggctgca accggtgcat ggttatgccg gcgtgggcat cattcgcgcc gccgaccgtt
     1981 ttgtcgcggc acactgtgaa acgcaagagt atgcgctggc gcagcgtttt cccactctgg
     2041 aggtggacta cccgctgaag catgcgttgt ggacccgcga aggcatcaag ggtggcaact
     2101 ggctgacgat tttgtccgac gccatggtgc agcaactgga aaaaatcagc gggtcgctgg
     2161 cattaccggc atccctctcc cgccatccct acgcgggtgg cttgat
//
LOCUS       DASUEG010000481         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1880155, whole genome shotgun sequence.
ACCESSION   DASUEG010000481 DASUEG010000000
VERSION     DASUEG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880155"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VFX11_08995"
     CDS             complement(<1..176)
                     /locus_tag="VFX11_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017188908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4938798.1"
                     /translation="MLGLVLFLGVMVLIALFFVGIFNRLVTLRNRFKNAFSQIDVQLQ
                     RRYELIPNLVETAKA"
     gene            complement(285..1190)
                     /gene="cyoE"
                     /locus_tag="VFX11_09000"
     CDS             complement(285..1190)
                     /gene="cyoE"
                     /locus_tag="VFX11_09000"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX4938799.1"
                     /translation="MTMATVEEKPVAPLWRAYWELTKPGVVALLMVTAVIGMFLSTPG
                     MVPWRVLVLGSLGMALAMMASAVINQIMDQRIDAIMARTHQRPLVQGRVNTGAALAFA
                     AVLCVLSLVILEVFINRLTAVLTFFGIIGYAFVYTMWLKRATPQNIVIGGLAGAIPPL
                     LGWTAVTGEVHPHALLLVLIIFAWTPPHFWALAIHRREEYAKADIPMLPVTHGLEFTK
                     TSVLLYTVILFLTALLPYLTGMSGLIYLAGATILNGMFLVYAVRLKFRDDPKVPMKTF
                     GFSITYLFLLFLILLVDHYFSFRLV"
     gene            complement(1187..2218)
                     /locus_tag="VFX11_09005"
     CDS             complement(1187..2218)
                     /locus_tag="VFX11_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX4938800.1"
                     /translation="MAEPISTARLWLTRLAVISVLITAVVVALGAWTRLEDAGLGCPD
                     WPTCYGSITVPLSEQAIQHGQALYPDSPFSAEKAWPEMIHRHFAKTIGLLCIIMAGLA
                     LWAARREKDIPVGHAVFLLFMVCLQGAFGAWTVTMKLFPPVVTGHLLFGFATFTTIVL
                     LVVRLSPFLRNSANAGARSLLPLAVLALLVLVGQIALGGWTASNYAATVCTELPVCQG
                     GWQQVLDFPEAFAIHTYAGTTYEFAPHLGPAEKITIHVAHRIGAWVTTALLTVTALMM
                     WVRAGGERYRRFAVFLGGILLVQVLLGGSNVVFQLPLAVAVAHNVVALVLLQVMVLLV
                     FSLYGEKRA"
     gene            complement(2211..>2442)
                     /locus_tag="VFX11_09010"
     CDS             complement(2211..>2442)
                     /locus_tag="VFX11_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938801.1"
                     /translation="AEFPEVARVDADSSQVNSALARVTEGATEAGNLFIMDPMGWIMM
                     SYLPEADEKESVIKAEDILNDLKKLLKASRIG"
BASE COUNT          632 a          806 c          606 g          398 t
ORIGIN      
        1 gccttggccg tttccaccag attgggaatc agctcgtaac gacgctgcaa ctggacatca
       61 atctgggaaa acgcgttctt gaaacggttc cgcaaagtga caagcctgtt gaatatgccg
      121 acaaagaaca gcgcaatcag caccattaca cccagaaaca ataccagtcc cagcatgatc
      181 actccctcaa tccggtagca gcaccagcac tgcccgacgc aaccataaca gcacggctac
      241 accccgaaca gtctttacgt cacaaagcag acacacgccc cttttcagac cagccggaag
      301 gagaaatagt gatccaccag caggatcagg aacagcagaa agaggtaggt gatggagaac
      361 ccgaacgttt tcatcggcac cttcggatca tccctgaact tgaggcgaac cgcatacacc
      421 agaaacatgc cgttcaggat ggtcgcccct gccagataaa tcaggccact catgccggtc
      481 agatagggca acaatgccgt caggaacagg atcaccgtat acaacagaac cgacgtcttg
      541 gtgaactcca gcccatgcgt cactggtagc atcggaatat cggcctttgc atactcttca
      601 cggcggtgaa tggccagcgc ccagaaatgc ggaggcgtcc aggcaaaaat gatcaggacc
      661 agcagcagcg catgcgggtg cacctcaccg gtcaccgccg tccaccccag caatggcgga
      721 atggcaccgg caagcccgcc aatcacgatg ttctgcggcg tcgcccgctt caaccacatc
      781 gtatagacaa aggcgtagcc gatgatcccg aaaaacgtca gcaccgctgt aaggcgattg
      841 atgaacacct ccaggatcac cagcgagagc acgcacagta cggcggcaaa tgccaatgcc
      901 gcccccgtat tgaccctgcc ctgcaccagc ggccgctgat gcgtacgcgc catgatcgcg
      961 tcaatgcgct ggtccatgat ctggttgatc actgccgacg ccatcatggc cagcgccatg
     1021 cccaatgacc ccaggacaag cacacgccac ggcaccatac cgggcgttga aaggaacatg
     1081 ccgatgacag ccgtcaccat gagcaacgcc actacacccg gcttggtcag ctcccaatag
     1141 gcacgccaca aaggcgcaac cggcttctct tccactgtcg ccatggtcat gccctttttt
     1201 ccccgtaaag actgaacacc agcaacacca tcacctgcag caacaccagc gcgacgacat
     1261 tgtgtgccac ggccaccgcc agcgggagct ggaaaacaac attggacccc cccagcagca
     1321 cctgcaccaa caggataccg cccaggaata cggcaaaccg gcgataccgc tcgccacccg
     1381 cccggaccca catcatcagc gctgtcaccg tcagcaaggc ggtggttacc catgcaccaa
     1441 tacggtgggc aacatgaatg gtgatctttt ccgccggtcc caggtgagga gcaaactcat
     1501 aggtcgtgcc agcgtaagtg tggatcgcaa acgcctccgg aaagtcgagt acctgctgcc
     1561 aaccgccttg gcacaccggc aactccgtgc agaccgtcgc ggcatagttg gaggccgtcc
     1621 acccgcccag ggcaatctgc ccgaccaata ccaacagcgc cagaaccgcc aacggcaaca
     1681 gcgatcgcgc acctgcatta gcactgttgc gcaggaaggg agacagcctg acaacaagca
     1741 ggacaatcgt tgtaaacgtt gcaaagccaa acagcaggtg accggtaacg accggcggaa
     1801 acagtttcat tgtcaccgtc cacgcaccaa acgccccctg caggcagacc atgaacaaca
     1861 aaaacacggc atggccgaca ggaatatcct tctccctgcg ggctgcccat agcgccagcc
     1921 ctgccatgat gatgcaaagc aggccaatgg tcttggcaaa atgccggtga atcatttccg
     1981 gccatgcctt ctccgcagaa aacgggctgt caggatacag agcctgacca tgctggattg
     2041 cctgctcgga caacggcacc gtaatcgacc catagcaggt cggccagtcg ggacagccca
     2101 acccggcatc ctcaagccgg gtccatgccc ccagtgccac cacaacggca gtaatcaaca
     2161 cactgataac agccaggcgc gtcagccaga gacgcgcagt agaaatcggt tcagccaatt
     2221 cgggacgcct tcaacagttt tttcaggtca ttgagaatgt cttccgcctt gatgacggat
     2281 tccttttcgt ccgcctcggg cagatagctc atcatgatcc agcccatcgg atccataatg
     2341 aacagattgc cggcctccgt tgcaccttcc gttacgcgcg ccagtgccga gttgacctga
     2401 ctgctgtcag catcaaccct ggccacctcc ggaaactccg cc
//
LOCUS       DASUEG010000482         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1843519, whole genome shotgun sequence.
ACCESSION   DASUEG010000482 DASUEG010000000
VERSION     DASUEG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843519"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFX11_09015"
     CDS             <1..322
                     /locus_tag="VFX11_09015"
                     /inference="COORDINATES: protein motif:HMM:NF044441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938802.1"
                     /translation="LKQLQIGLQQGKPTWPIPQPLTRQQQDDYKELRNVLASVGQELG
                     IVPDLLASKAQLSDYIQDRAEGQDGSDVFTGWRAQVLLPRLNAHPLASAPATASGGNE
                     PNEH"
     gene            312..596
                     /locus_tag="VFX11_09020"
     CDS             312..596
                     /locus_tag="VFX11_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HEX4938803.1"
                     /translation="MSTEHRQSGDSVLCAVYRSPKKEGMYLYVPKTENPFEQVPEPLL
                     QRFGEPSWVLDLELTASRKLARVSAADVIKGLRDLGFYLQMPPGDLPREI"
     gene            596..1036
                     /locus_tag="VFX11_09025"
     CDS             596..1036
                     /locus_tag="VFX11_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX4938804.1"
                     /translation="MPARFWEHKTLAEMNPDEWESVCDGCGLCCLQKLEDEDSGEVFY
                     TDLACTLLDLNTCRCGDYPNRLARVPDCLRLDLTNLPAFHWLPASCAYRRLHEGRGLA
                     DWHPLVSGDPDSVHKAGISVRGRAVSMNSVAEEDWEDHIIHWVN"
     gene            1047..1391
                     /locus_tag="VFX11_09030"
     CDS             1047..1391
                     /locus_tag="VFX11_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938805.1"
                     /translation="MLEYSENNVAWVAYCAGAVGCLLVWWRMTRILPWSPLRQLLRIL
                     VAALLLVPAPVAPGREELAPAFVVLLFDTTLVKQGDPLHAAPYLIYGLMLGLVILVID
                     GFVRFMLSRRHA"
     gene            1506..>3134
                     /locus_tag="VFX11_09035"
     CDS             1506..>3134
                     /locus_tag="VFX11_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX4938806.1"
                     /translation="MKLPPLPGLLWIILLWALFYGAQTVFAEEKTAAPPDLRVLIDVS
                     GSMKKTDPNNLRVPAVKLLLNLAQDGSQLGIWMFDQGVKSLVPLGKVTADWKKTAAGK
                     VDAIHSRGLFTGIGAALEAAARGQIKPDPAWDRTIVLLSDGMVDIAKNPAVNERERKR
                     ILDEIVPKLKAGGFRIRTVALSDQADQSFLRSLALNTGGSFVIARNADELLKAFVTTA
                     DKVNLPEQVPIEGDTFAIDESVKEFTALIFRKAGGKPTELVAPDGLHFSMSKASRNVS
                     WFADQRYDLITVYNPVPGKWKVAGDLDPSNRVTVVSDLQLEVEGLPDSVLEGEKITMR
                     MHLAEHGRGIANPNFLELMDITFSQQTASGETFEGKLSRNADGTSNVPADGVYSARLG
                     RTLLAGDHTFTLLVDGKTFKRKKQYNMTVYRDVLDVKTEYRDENGQVVQLLVAEPRGG
                     MVKAESLQLAAKITDPNGKETLQPVKQRDDGRWQVDVLPFGAQGVYQVALTVKGTSLS
                     GRQFEMLQGPYPVDYTPLAQSLADAPATVKTDKAP"
BASE COUNT          627 a          963 c          986 g          558 t
ORIGIN      
        1 gctgaaacag ctgcagatcg gcctgcaaca gggcaagccc acttggccca tcccgcagcc
       61 gctcacccgg cagcagcagg acgactacaa ggaactgcgc aacgtcctgg ccagcgtggg
      121 gcaggagctg ggcatcgtgc cagatctgct ggccagcaag gcccagctgt ccgattacat
      181 tcaggatcgc gccgagggcc aggatggcag tgacgtgttc accggctggc gcgcccaggt
      241 gctgctgccc cgtctgaacg cccatcctct ggcatccgcc cccgcaacgg cttctggtgg
      301 aaatgaaccc aatgagcact gaacatcgtc aaagcgggga cagcgttctc tgcgcggtct
      361 accgcagtcc gaaaaaggaa ggcatgtacc tgtatgtgcc gaaaaccgaa aaccccttcg
      421 agcaggtgcc cgaaccgctg ctgcagcgct ttggtgaacc atcctgggtg ctggacctgg
      481 agctgacagc gtcccgcaag ctggcccgcg tcagtgccgc cgacgtcatc aagggcctgc
      541 gggaccttgg tttctatctg cagatgcccc ccggcgattt gccccgggag atctgatgcc
      601 ggcgcgcttc tgggaacaca agaccctggc ggaaatgaac cctgacgagt gggaatccgt
      661 ctgcgacggt tgtggcctgt gctgtctgca aaagctggaa gacgaggaca gcggcgaggt
      721 gttctacacc gatctggcct gcaccctgct ggatctgaat acctgccgct gcggcgatta
      781 ccccaaccgg ctggcgcggg tgccggactg tctgcgtctg gacctgacca atctgccggc
      841 attccactgg ctgcctgcca gctgcgccta ccgtcgcctg cacgaggggc gggggctggc
      901 ggactggcat cccctggtgt ccggcgatcc ggacagcgtc cacaaagcgg gcatttccgt
      961 gcggggtcgc gccgtttcca tgaacagcgt cgcggaagaa gactgggaag accatatcat
     1021 tcattgggtg aactaaggga atccctatgc tggaatactc tgaaaacaac gtggcctggg
     1081 ttgcgtactg cgccggtgcc gtgggttgcc tgctggtgtg gtggcgcatg accagaatct
     1141 tgccctggag ccccctgcgc cagctgctgc gcatcctggt ggcggctctg ctcctggtgc
     1201 cggccccggt ggctccgggc agggaagaac tggcaccggc ctttgtagtg ctgctgttcg
     1261 atacaaccct ggtgaagcag ggtgatcccc tccatgccgc accctacctg atctacgggc
     1321 tgatgctggg gctcgtgatc ctggtcatcg atggattcgt ccgcttcatg ctgtccagac
     1381 ggcacgcctg aggttctgcc gaaaatcagg cggttgcccg tcactattac cttttccgtc
     1441 aggaaaacac cggcaaagct gctataacta aacagtgttg gtagcgcacc tgtcacggag
     1501 ttttcttgaa actgcccccg cttcccggcc tgctgtggat aatcctgctc tgggcgctgt
     1561 tttatggcgc ccagacggtg tttgccgagg aaaaaacggc tgccccgcct gacctgcggg
     1621 tgctgatcga cgtgtccggc agcatgaaga agaccgatcc gaacaacctg cgggtgccgg
     1681 cggtgaaact gctgctgaac ctggcccagg acggcagcca gctgggcatc tggatgttcg
     1741 accagggggt gaaatccctg gtgcccctcg gcaaggtgac agcagactgg aaaaagacgg
     1801 cggcgggaaa ggttgacgcc atccattccc ggggtctgtt caccggaatt ggtgccgcgc
     1861 tggaggccgc cgcccggggc cagatcaagc ccgatcccgc ctgggaccgc accattgtcc
     1921 tgctgtcgga tggcatggtg gacatcgcca agaatcccgc cgtcaacgag cgtgaacgca
     1981 agcgcatcct ggatgagata gtgccgaaac tgaaagcggg cggctttcgc atccgcaccg
     2041 tcgccctgtc ggaccaggcc gaccagtcct tcctgcgcag cctggcactg aataccggcg
     2101 gcagcttcgt gattgcccgc aatgccgatg aactgctgaa agccttcgtc accactgccg
     2161 acaaggtgaa tctgccggag caggtcccga tcgagggcga caccttcgcc atcgatgaat
     2221 ccgtgaaaga attcaccgcc ctgattttcc gcaaggccgg cggcaagccc accgaactgg
     2281 tggcacccga cggcctgcat ttcagcatga gcaaggccag ccgcaacgtc agctggtttg
     2341 ccgaccagcg ctatgatctg atcacggtct acaaccctgt acccggaaaa tggaaagttg
     2401 ccggggatct ggacccgtcc aaccgtgtca cggtggtgtc cgacctgcaa ctggaagtgg
     2461 aagggctgcc ggacagtgtt ctggaaggcg aaaagatcac gatgcgcatg catctggcgg
     2521 agcacggccg tggcatagcc aacccgaatt ttctcgagct catggacatc accttcagcc
     2581 agcagaccgc cagcggcgaa accttcgagg gcaagctgtc gcgcaatgcc gatggcacca
     2641 gcaatgtgcc ggcggacggc gtctacagcg cccggctggg ccgcacattg ctggccggcg
     2701 accacacctt cacactgctg gtggatggca agaccttcaa gcgcaagaag cagtacaaca
     2761 tgacggtgta ccgcgacgtg ctggatgtga aaaccgaata ccgtgacgag aacggtcagg
     2821 tggtgcagct gctggtggca gagccgcggg gcggcatggt aaaagccgaa agtctgcagc
     2881 tggcagcgaa aattaccgac cccaacggca aggaaaccct gcagccggtg aagcagcgag
     2941 acgatggccg ctggcaggtg gacgtgttgc cctttggcgc ccagggcgtc taccaggtgg
     3001 cgctgacggt gaagggtaca tccctcagcg gacgccagtt tgaaatgctg cagggcccgt
     3061 acccggtgga ttacacgccg ctggcgcaga gcctggctga tgcgccggcg acagtgaaaa
     3121 cagacaaggc accc
//
LOCUS       DASUEG010000483         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1703978, whole genome shotgun sequence.
ACCESSION   DASUEG010000483 DASUEG010000000
VERSION     DASUEG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703978"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFX11_09040"
     CDS             <1..930
                     /locus_tag="VFX11_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4938807.1"
                     /translation="CSIGGAADRLGMTQPAVSNAVARMRQVWNDPLFVKDGRGISPTA
                     RAQSLWSEIRSPLGQLKEAVAPGAFDPATSARRFRVAMSDIMVDLLWPPMRQLVEREA
                     PGIDLHAVPYMPMSASQLLADATADLVIAPLDQFRASDRREVMLRRDYVCAMRRGHPL
                     SGRPLTLENYLAADHLMVSLSGDPTSFVDETLQRQGLRRRVAMTVNHFAAVPRLLKRT
                     NLIALTVNTLIAEDVLAGHLVVTTPPLALDTAEIAIGWHPRHDRDAGVCWLRHHLVRL
                     AQSQWKRFSPYCTLCPDAVKGAGPDPDDPLAVA"
     gene            complement(952..1278)
                     /gene="trxA"
                     /locus_tag="VFX11_09045"
     CDS             complement(952..1278)
                     /gene="trxA"
                     /locus_tag="VFX11_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX4938808.1"
                     /translation="MSDHIRTLNDTDFSQTVQQASQPLLVDFWADWCGPCHAIAPVLD
                     ELARDLNGKVTFAKVNVDQNPETTARFGIRSIPALFLFKDGQAVARHAGVASYKELKS
                     FITGAL"
     gene            1480..>2076
                     /locus_tag="VFX11_09050"
     CDS             1480..>2076
                     /locus_tag="VFX11_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4938809.1"
                     /translation="MKNQELTLLYVFDAIMTEGSVTRAADRLAMTQPAVSNAVSRMRE
                     IWNDPLFVKKGRQIEPTAFALSLWEQVRGPMHDLSSAVDAGAFDPAQSRRKFRIAMGD
                     ASVDLYWLPLTSEVARVAPHVDLYTVPFTMDGAVNQLREANVDLALGPMTQHDRSLRS
                     IWLFESRYILAMRKDHPLAGRPVSLDDYLAARHLLVTTS"
BASE COUNT          331 a          670 c          697 g          378 t
ORIGIN      
        1 tgctccatcg gtggcgcggc ggaccggctg ggcatgaccc agccggcggt atccaatgcg
       61 gtggcgcgca tgcggcaggt ctggaacgat cccctgtttg tgaaggatgg ccgtggcatc
      121 agccccaccg cccgggcgca aagcctctgg tccgagatcc gttcgccatt ggggcagctg
      181 aaagaagcgg tagcgccggg cgcattcgat ccggccacca gtgcccgtcg ttttcgggtg
      241 gccatgtccg acatcatggt ggatctgctg tggcccccca tgcgccagct ggtggagcgg
      301 gaggcaccgg gcatcgatct gcacgccgtg ccctacatgc cgatgagtgc cagccagtta
      361 ctggcggacg ccactgccga cctggtgatc gcgccgctgg accagttccg cgccagcgac
      421 cgccgtgaag tcatgctgcg gagggactat gtctgcgcca tgcgccgggg ccatccgctg
      481 tcgggccgac ccctgactct ggaaaattac ctggcggcgg accacctgat ggtgtccctg
      541 tccggggatc ccaccagctt tgtggatgaa accctgcagc ggcagggtct gcgccggcgg
      601 gtggcgatga cggtgaatca tttcgccgcc gtgccgcgtc tgctcaagcg caccaacctg
      661 attgccctca ccgtcaatac cctgatcgcc gaggacgtgc tggccggcca tctggtcgta
      721 acgacgccgc ccctggccct ggataccgcc gagatcgcca tcggctggca tccgcgccac
      781 gaccgcgacg ccggtgtctg ctggctgcgg catcatctgg tgcggctggc ccagtcccag
      841 tggaagcggt tcagccctta ctgcaccctg tgtcccgatg ccgtgaaggg cgccgggccg
      901 gacccggatg acccgctggc ggtggcctga gcgggtccgg tgccggccgg ttcagagagc
      961 gccggtgata aaggatttca gttccttgta cgacgccacg cccgcatgcc gtgccaccgc
     1021 ctggccgtcc ttgaacagga acagggcggg aatgctgcgg atgccgaacc gggcagtggt
     1081 ttcgggattc tggtccacgt tgaccttggc aaaggtgact ttgccgttga ggtcgcgggc
     1141 cagttcatcc agtaccggcg cgatggcgtg gcaggggccg caccagtcgg cccagaagtc
     1201 caccagcagt ggctggctgg cctgctgcac ggtctggctg aagtcggtgt cgttgagggt
     1261 gcggatgtga tcgctcatgg tgggtacctc ccgttgggtg attgctgcgg ttttcaccac
     1321 aatgggcgcc ggccaggctg aactcaaatt gacagcggat atagacgaca tcacccgatg
     1381 tgatggaacg gccgccgagt aacacccgat tggatgacca gctgacgtct ggttatcacc
     1441 tgacgtttga ttttgcctga cctgacccgg gagatcgccg tgaagaacca ggaactcacc
     1501 ctgctgtatg tattcgacgc catcatgacc gaaggctcgg tgacccgggc cgccgaccgg
     1561 ctggccatga cacagccggc ggtgtccaac gccgtgtcgc ggatgcggga aatctggaac
     1621 gacccgctgt tcgtgaagaa ggggcgccag atcgagccca cggcctttgc cctcagcctg
     1681 tgggagcagg tgcgcggccc catgcacgac ctgtccagcg cggtggacgc cggtgcgttc
     1741 gatccggccc agtcccggcg caagttccgc atcgccatgg gggatgcctc ggtggacctg
     1801 tactggctgc cgctcacgtc cgaggtggca cgggtggcgc cccatgtgga tctctacacg
     1861 gtgccgttca ccatggacgg cgccgtcaac cagctgcggg aggccaacgt ggatctggcc
     1921 ctgggcccca tgacccagca tgaccgcagc ctgcgttcca tctggctgtt cgagagccgt
     1981 tacattctgg ccatgcgcaa ggaccatccc ctggcgggcc ggccggtgag cctggatgac
     2041 tatctggcgg cgcggcacct gctggtgacc acgtcc
//
LOCUS       DASUEG010000484         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1371412, whole genome shotgun sequence.
ACCESSION   DASUEG010000484 DASUEG010000000
VERSION     DASUEG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371412"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFX11_09055"
     CDS             <1..581
                     /locus_tag="VFX11_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4938810.1"
                     /translation="YSLSSIKELISALENGIDLRHLMGLESALTSPWTDEEPMLVPMT
                     ELLVMFGGSLTPEALLRANELDLIKPEGTMMRVRSMRTLRAGAELVATGIPLEDLLDI
                     VRMLRGNVERVANELVRLVGDHVLSNYETQELPPTDDFPKLADLVWRLRPLAEMAVHA
                     ELARAMETAASRLLSDRLEKIIERMNREKSGK"
     gene            complement(660..1517)
                     /locus_tag="VFX11_09060"
     CDS             complement(660..1517)
                     /locus_tag="VFX11_09060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4938811.1"
                     /translation="MTAAILPVRRNLQFHLPQNRISNWNGHGTHWTHFLNTLSIFFPA
                     GERFFIQSIRNYRDQITDGDLKKAVAAFIGQEAFHTREHEEYNEAMVAAGLQTDKMEK
                     SVEELLAFLQKNLPKSFQLAATVALEHLTAMLAEMVLTDDRFFVGSEERYHALWQWHA
                     MEETEHKGVAYDVYEQTVGKGPKAYAIRATAFLVANVIFWSLFYRYYYKVLKAEKQHL
                     NIKGWLKSFNFQFGRDGLLPRMLPEWLDFFRPSFHPWDKDNRFHLQRAEELMKKVDAF
                     AEQVGLAAA"
     gene            complement(1826..>2664)
                     /locus_tag="VFX11_09065"
     CDS             complement(1826..>2664)
                     /locus_tag="VFX11_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4938812.1"
                     /translation="ERSLIRFLLEQGFSVYMVDWGSPTRDHAHYTLSTYARKLLPAFL
                     QQVRVHSGQTQLTLHGWSMGGGIALVYVALSGDRDIRNLITVGTAVDGHANGPIGRQY
                     AALNRVLQRAGVNFRKLPARWAYTPGWANAIGFKLSDPVGSVRGYLDLLRNLDDREYV
                     SQHATQGAFIDRLEAYPGGIIRDWTYSVWLENEAGRGQFSVGRDIARLDRIQANLFCV
                     AGQSDTLANIDSCKALLELVSSQDKQLFIAPGGHTGIVSGSQAPETVWRAMTDWLATR
                     SD"
BASE COUNT          570 a          766 c          792 g          536 t
ORIGIN      
        1 ggtattcact ctccagcatc aaggaactga tcagcgcgct ggaaaatggc atcgacctgc
       61 gccacctcat gggccttgaa tcggccctca ccagtccgtg gactgatgaa gagcccatgc
      121 tggttcccat gaccgaactg ctcgtcatgt tcggtggcag cctcacgccg gaagcgttgc
      181 tgcgggccaa tgaactggat ctgatcaaac cggaaggcac catgatgcgg gtgcgcagca
      241 tgcgcacatt gcgggccggc gcagaactgg tggccaccgg cattccactg gaggatctgc
      301 tggacatcgt gcgcatgctg cgcggcaacg tcgagcgggt ggccaatgag ctggtgcggc
      361 tggtgggcga tcacgtgctg tcgaactatg aaacccagga actgccgccg acagacgact
      421 tccccaagct ggcggatctg gtgtggcgac tgcggccact ggctgaaatg gccgtgcatg
      481 ccgaactggc gcgggcaatg gaaaccgccg catcgcgact gttgtccgac cggctggaaa
      541 aaatcatcga acgcatgaac cgggaaaaat ccggcaaata aatcccgcct gcaaaacgaa
      601 aaaaggctgt gcggaaaagc acagcctctg gcaaaagtcg ccgttaaccg tctgtcagat
      661 caggccgctg ccaggcccac ctgttcagca aacgcatcca cctttttcat cagctcttca
      721 gcacgctgca ggtggaaacg gttgtccttg tcccagggat ggaaactcgg acggaagaaa
      781 tccagccact ccggcaacat gcgcggcaac agaccatcgc gaccgaactg gaagttgaac
      841 gacttcagcc agcccttgat attcagatgc tgtttctccg ccttcaaaac cttgtagtaa
      901 tagcggtaga acaggctcca gaaaattacg ttggctacca ggaacgctgt cgcgcggatg
      961 gcgtaggcct tcggcccttt gccaacggtc tgttcgtaca cgtcgtacgc cacgcctttg
     1021 tgttcggttt cttccatcgc gtgccactgc cacagcgcgt gatagcgctc ttcgctgccg
     1081 acgaagaaac ggtcgtcagt cagcaccatt tccgccagca tggccgtcag gtgttccagc
     1141 gccactgtcg cggccagctg aaatgacttc ggcagatttt tctgcaggaa ggccagcaat
     1201 tcctcgacgg acttttccat cttgtcggtc tgcagaccgg cggccaccat ggcctcgttg
     1261 tactcttcat gctcgcgggt atggaacgct tcctgcccga taaaagcggc gacagccttc
     1321 ttcagatcgc catcggtgat ctggtcacga tagttgcgaa tgctctggat gaagaagcgt
     1381 tcaccggcag ggaagaaaat ggagagggtg ttgagaaagt gggtccagtg agtgccgtga
     1441 ccattccagt tgctgatccg gttctggggc aaatggaact gcaggttacg acgcacgggc
     1501 aggatggctg ctgtcatagg tttccctctg tatgtctcat tgttttatgt tacacgagtg
     1561 aatgtaatat aaaacctact gcgggtacag tggtgaatac caccaaatat gtaacattat
     1621 tttatgtcac attgctgatg tatataggag gaagacattc tggtcaagaa ttgcgcacgg
     1681 aattgccaca aaaatgtacg cttgtggcgc atgccggcta cggagtggta acgcgtgctc
     1741 tgtacggggg cacttgtttt cggcagaaac ggcgggatga atacaaaaaa taccccggtt
     1801 accgcaaggt ccggggcgct cgaatttaat cagaacgggt ggcaagccag tccgtcatgg
     1861 cacgccagac ggtttccggc gcctgactgc cactgacgat gccggtgtgg ccaccaggcg
     1921 cgatgaacag ctgtttgtcc tggctgctga ccagttccag cagggctttg cagctatcga
     1981 tattggccag ggtgtcgctc tggcccgcca cgcagaacag gttggcctgg atgcggtcca
     2041 gccgcgcaat atcccgtccc accgaaaact gcccccgccc cgcttcgttt tccagccaga
     2101 cgctgtaggt ccagtcgcgg atgatgccgc cgggataggc ttccagccgg tcgataaagg
     2161 cgccctgggt tgcatgctgg gacacatact cgcggtcgtc caggttgcgc agaagatcca
     2221 gatagccgcg cacactgccg accgggtcgg acagcttgaa accgatggcg ttggcccagc
     2281 ccggtgtgta ggcccagcgc gccggcagct tgcggaaatt cacccctgcc cgctgcaata
     2341 cccgattgag ggcggcgtac tggcggccga tggggccgtt ggcgtgaccg tcaaccgccg
     2401 tacccacggt aatcagatta cggatgtcgc gatcgccact gagggcgaca tagaccagcg
     2461 cgatgccgcc gcccatgctc cagccatgca gggtcagctg cgtctggccg ctgtgtaccc
     2521 gcacctgctg cagaaaggcg ggcaacagtt tcctggcgta ggtggacagc gtgtagtggg
     2581 cgtgatcgcg cgtggggctg ccccagtcca ccatgtaaac agagaagccc tgctccagca
     2641 ggaagcggat caggctgcgc tcgg
//
LOCUS       DASUEG010000485         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_753464, whole genome shotgun sequence.
ACCESSION   DASUEG010000485 DASUEG010000000
VERSION     DASUEG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753464"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VFX11_09070"
     CDS             complement(<1..1275)
                     /locus_tag="VFX11_09070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4938813.1"
                     /translation="MSNPAVATPTLDSSGQSNPTQAPAYPAAPKRFLPWSIAGRKWLP
                     DFIAKPFFGKPLAPSQEEWQRMERALWQGDPAMDKVVDWMFEVGPRHSKPLFDQALQH
                     GIDSLTDPPQVLREFFAIVDQAPPWLNRTRLEKGVKVAHLTGTVSFYVLRDMALMGGY
                     AYFNSMNQTLAATGALSKDTALRLGETGKWLNDVTEPHGMERFGPGFITTLRVRLVHA
                     LVRRHLQNKADWEPEKWGVPINQVDMLATYLAFGPATLMGARMFGLPIWPRQGAAVMH
                     LWRYIGWLMGVDEQWLALSEFDGLRKLLHTFMTHRLPDDKIRQLGQALRDEPLSRHLP
                     GLEQHPRRARFRHWFLYQMHLSNSSLILGPIQRRRLGLPMTILPWYPAATAPFRFIKL
                     FYCQLRGGQALENYTRRSREQQTELLVHYFGER"
     gene            1383..2003
                     /locus_tag="VFX11_09075"
     CDS             1383..2003
                     /locus_tag="VFX11_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938814.1"
                     /translation="MRKVPRQKRSRAMVARLLDATAITLAERGLDGTTTNHIAEQAGV
                     SIGSLYQYFPDKDALVEALLERMGNDISQNFRARAAAVDFNQLRLRNIASFSIAYGMN
                     LIKTDPLIREVTRNWNRVPINKLLDPLERFFLVMALPYFVNHYRDYPVQNLEAKLYVL
                     INSTLFTTIRFLVQENTLLPEAVLIRTLTDMIVALLELPAVQPDIV"
BASE COUNT          405 a          658 c          705 g          450 t
ORIGIN      
        1 ccgctcgccg aaatagtgca ccagcagttc tgtctgctgc tcgcggctgc ggcgggtgta
       61 gttttcaaga gcctggccgc cgcgcaactg gcaatagaac agcttgatga agcggaaggg
      121 ggccgttgcc gccgggtacc agggcaggat ggtcatgggc aggcccagtc gccggcgctg
      181 gatcgggccc aggatcaggg atgagttgct aaggtgcatc tggtagagga accagtgccg
      241 gaaccgggcg cggcgggggt gctgctccag gccgggcagg tggcggctga gaggttcatc
      301 gcgcagggcc tgccccagct ggcggatctt gtcatctggc aggcggtgag tcatgaaggt
      361 gtggagcagt ttgcgcagtc catcgaattc ggacagcgcc agccattgct catccacgcc
      421 catcagccag ccgatatagc gccacagatg catgacagcc gcgccttgcc ggggccagat
      481 gggcagcccg aacatgcgcg cgcccatcag ggtggccggc ccgaacgcca gataggtggc
      541 cagcatgtcc acctggttga tcggaacgcc ccatttttcc ggttcccagt ctgccttgtt
      601 ttgcaggtga cgccggacca gcgcatgcac cagccgcacc cgcagggtgg tgatgaagcc
      661 gggcccgaaa cgctccatgc cgtggggttc ggtgacatcg ttcagccatt tgcccgtctc
      721 gcccagtcgc agggccgtgt ctttgctcag ggcgccggtg gccgccaggg tctggttcat
      781 gctgttgaaa taggcgtacc cgcccatcag ggccatgtcc cgcaacacat aaaagctcac
      841 cgtgccggtc aggtgtgcga ccttcacgcc cttttccagc cgtgtccggt tcagccacgg
      901 cggtgcctgg tccacgatcg cgaagaattc ccgcagtacc tggggcggat cggtcaggga
      961 atcgatgccg tgctgcagtg cctggtcgaa cagcggtttg ctgtgacggg gccccacttc
     1021 gaacatccag tccaccacct tgtccatggc ggggtcgccc tgccagaggg cgcgctccat
     1081 gcgctgccat tcttcctggc tgggcgccag cggcttgccg aaaaacggct tggcgatgaa
     1141 gtccggcagc catttccgcc cggcgatgga ccagggcagg aagcgtttgg gcgccgccgg
     1201 gtatgccggc gcttgtgtgg ggttcgactg cccggatgag tccagggtag gggtggcaac
     1261 tgcagggttg gacattgcgg tggattcgct cttgcaaaac ggacaggtcg atcctaattt
     1321 attacgagta tttgctcgca ttcaactcgc ttttttggat tttgacctga ccggagtcag
     1381 tcgtgagaaa ggtaccccga cagaaacgat cccgcgcgat ggtggcccgt ctgctggatg
     1441 ccaccgccat cacgctggcg gagcgcgggc tggacggtac caccaccaat catattgcgg
     1501 agcaggccgg cgtcagcatc ggttccctgt accagtattt tcccgacaag gacgccctgg
     1561 tggaggcgct gctggagcgt atgggcaatg acatcagcca gaacttccgc gcgcgggcgg
     1621 cggcggtgga cttcaaccag ctgcggttgc ggaacatcgc gtctttttct atcgcgtacg
     1681 gcatgaacct gatcaagacc gatccgctga tccgggaggt gacccgcaac tggaaccggg
     1741 tgccgatcaa taaactgctc gatccgctgg agcgcttttt tctggtaatg gcactgccgt
     1801 atttcgtcaa tcactatcgc gattatccgg tacagaatct ggaggcgaag ctgtatgttc
     1861 tgatcaactc cacgctgttc acgacaatcc ggtttctggt gcaggaaaat acgctgttgc
     1921 cggaagccgt gctgatccgg acgctgacgg acatgattgt ggcgctgctg gaactgccgg
     1981 cggtgcagcc ggatatagtt tgatatgaat caagaatggg gtaatgttca aagcctatgc
     2041 tggctgttcg tcaaaagcgc gcaatgcgtt ttttgccaag gattcccgcc aaggctgccc
     2101 ttcaaggaga gcttcaatgt ccgcccccac cacggctcca tccactcatt cccgcaccgc
     2161 acttgatacc cagtccacgc cgctgggtgc tgacgaactg cgccgtctgc atgcctgg
//
LOCUS       DASUEG010000486         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_497130, whole genome shotgun sequence.
ACCESSION   DASUEG010000486 DASUEG010000000
VERSION     DASUEG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497130"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFX11_09080"
     CDS             <1..372
                     /locus_tag="VFX11_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4938815.1"
                     /translation="WSRILQRIHAPGPAGTLTVRANEGEWIACLPGVDVRILYADEKM
                     NTQTALWRMQPGSMLPAHTHAVDEECLVLEGAIRSGDFVVRQGDYHLGFAGQPHPDIH
                     SDGGALLLIRSERKYLRSAHP"
     gene            complement(414..1655)
                     /locus_tag="VFX11_09085"
     CDS             complement(414..1655)
                     /locus_tag="VFX11_09085"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX4938816.1"
                     /translation="MIGELLGTVSTLWEGGERYVSATKVGWEIERFYRHYRQQALALE
                     PLEQRRLLAQTHYKGAVKLAKLFHDNGAIWVKFGQFLSSRTDILPMQYISELEKLQDD
                     AKPAAFRAIETVLVEEWGRDWRKRFARFDDMPVAAASVAQVHRAVLHTGENVAVKVQL
                     PHARRLFKQDSAVFKALATVGAPMISHFDLKQVIDQIVDMTLRELDFLQEEANHKKFD
                     SHPHSPLIHVPHLYEDMSSDRVLVTEWIDGVRLTDYLNRNPAKAELLLRELLSSYVQQ
                     ITAFGVYHADPHPGNFLVMNDGRVAVLDYGAIGELTQEETRNYALLLQVLFGKIQSPL
                     PLGELFRRAGFIARDQQVFEEVAELVLKEALRKSGSTDILGIALNRMRDLKVQIPDSF
                     VSVARVVLTFGGLLKTYGVSV"
     gene            complement(1815..>3154)
                     /locus_tag="VFX11_09090"
     CDS             complement(1815..>3154)
                     /locus_tag="VFX11_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938817.1"
                     /translation="ERMLARLADPGFLERYRAVTGDSSLSGFYDTRRRLQQQESQLDA
                     IQGPTPAMWFEVASRRIAALAGLVVDLDQQMRVELEAETAAARTRLWAEAIGLVLVLA
                     LVAWTTRWIGAGILHRVERVERALLQAEKEGRLNQTLEDDSDDEISIIARSLNRFFAS
                     IAGIIGHIVRVTLGLNQRAQDFRQLTGDNLQVLHRQQLETQQIASAITEMAASIQEVA
                     RSAQEATSLTHLAGDSTQTGQDKANATHHAVAQLTHELENAARQVGEVAQQSQKIGGI
                     LDTIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAQRTQESTAEIQRMIQ
                     QLQTGSDAVRSSMDTSRQHAERCNELAEESGAVLGQIGTLVHQVVEHMNQIAVAAQEQ
                     STVATQIDQNTQQIALSADTSLKSAEQVQKGSIQIQEQAAELQQLVVRFDVAR"
BASE COUNT          553 a         1002 c          970 g          629 t
ORIGIN      
        1 tggtcgcgga tactgcagcg cattcacgca ccggggccag ccggtacgtt gaccgtgcgg
       61 gcgaacgaag gtgaatggat tgcctgcctg cccggagtgg atgtcaggat tctgtacgcg
      121 gatgagaaga tgaacacgca aacggcgcta tggcgcatgc aaccgggttc aatgctgccg
      181 gcgcacaccc atgctgtcga tgaagaatgt ctggtgctgg aaggcgcgat ccggtcgggg
      241 gattttgtgg tgcggcaggg cgactatcac ctgggatttg ccgggcagcc ccacccggac
      301 atccactccg atggtggtgc attactgctg atccgctcgg agcggaagta tctgcgcagc
      361 gcccatccgt gaaccagtat taatgaaccg gcgcctgcgc tgaagggatc agatcagaca
      421 ctcactccat acgtcttcaa caacccgcca aacgtcagca ccacccgcgc caccgacaca
      481 aacgaatccg gaatctgcac tttcagatcc cgcatccggt tcagggcaat gcccaggatg
      541 tcggtggaac cggatttgcg cagggcttcc ttcaacacca gctccgccac ctcttcaaac
      601 acctgctgat cgcgcgcgat aaaacccgcg cgacggaaca gctcgcccag tggcagcggg
      661 ctctggatct tgccgaacag cacctgcagc aacagcgcgt aattgcgggt ttcttcctgc
      721 gtcagttcgc cgatggcgcc gtaatccagc accgccacgc ggccatcgtt catcaccaga
      781 aagttacccg gatgcggatc agcgtgatac acgccgaacg cggtgatctg ctgcacatag
      841 ctcgacagca gttcccgcaa caacagttcg gctttggcgg gattgcggtt caggtaatcg
      901 gtcaggcgca caccgtcgat ccactccgtc accagcacac ggtcactgct catgtcctcg
      961 tacagatgcg gcacatggat cagagggctg tggggatggg aatcgaattt cttgtggttg
     1021 gcttcctcct gcagaaaatc cagttcccgc agggtcatgt cgacgatctg gtcgatcacc
     1081 tgtttcaggt cgaaatggga aatcatcggc gcgccgacgg tggccagcgc cttgaacacc
     1141 gcggaatcct gtttgaacag acgacgggca tggggcaatt gcactttcac cgccacgttc
     1201 tcacccgtgt gcagcacggc gcggtgaacc tgcgccaccg acgcggcggc caccggcata
     1261 tcatcaaagc gggcgaagcg tttgcgccag tcacggcccc actcttccac cagtacggtt
     1321 tcgatggcgc ggaaggcggc gggcttggcg tcgtcctgca gtttttccag ttcggaaatg
     1381 tattgcatgg gcaggatgtc ggtgcgggaa ctgaggaact ggccgaactt gacccagatg
     1441 gcgccgttgt catgaaacag cttggccagc ttcaccgcgc ccttgtagtg ggtctgggcc
     1501 agcaggcgac gctgctccag cggttccagg gccagtgcct gctgacggta atggcggtaa
     1561 aaacgctcga tctcccagcc caccttggtg gcggatacgt agcgctcccc tccttcccac
     1621 agcgtactga cagtgcccaa cagttcccca atcatgctga ccccggctct gatttttata
     1681 atgttaaaaa atgtcggtcg gtgtttgttg ttggcgttca cgtaacagtg tgcctggagc
     1741 gactataaag aatgcccgaa tattgtctgt tgcggatttg ttgcgccccg taacggagcg
     1801 cccgcagcag cgggtcagcg tgccacatcg aagcggacca ccagctgctg cagctccgcc
     1861 gcctgttcct ggatctggat cgaacccttc tgtacctgct cggcactttt caggctggtg
     1921 tcggcactga gggcgatctg ctgggtgttc tggtcgatct gggtggcgac ggtggattgc
     1981 tcctgggccg ccaccgcgat ctggttcatg tgttccacca cctgatgcac cagcgtgccg
     2041 atctggccca gcacggcgcc ggattcctcc gccagttcgt tgcagcgctc ggcgtgctgc
     2101 cggctggtgt ccatgctgct gcgcacggca tcgctgccgg tctgcaattg ctggatcatg
     2161 cgctggattt cggcggtgga ttcctgtgtg cgctgcgcca gcgaccgcac ttcgtccgcc
     2221 accaccgcaa agccacggcc ctgctcaccg gcgcgggcgg cttcgatcgc tgcgttcagt
     2281 gccagcaggt tggtctgctc ggcgatgccg cggatggtat ccagaatgcc gccgatcttt
     2341 tgcgattgct gcgccacctc ccccacctgg cgggccgcgt tttccagttc gtgagtcagc
     2401 tgcgccaccg cgtggtgggt tgcgttggcc ttgtcctgcc cggtctgggt gctgtcgccc
     2461 gccagatggg tgaggctggt ggcttcctgg gcggagcggg cgacctcctg aatgctggcg
     2521 gccatctcgg tgatggcaga cgcgatctgc tgggtttcca gttgctgccg gtgcagaacc
     2581 tgcagattgt ccccggtgag ctggcggaag tcctgggcgc gctggttcag gcccagggtc
     2641 acgcgcacga tatggccgat gatgccggcg atgctggcga aaaagcggtt gagcgagcgg
     2701 gcgatgatgc tgatctcgtc atcgctgtcg tcctccaggg tctgattcag acggccctcc
     2761 ttttccgcct gcagcaacgc acgttccacc cgctccaccc ggtgcaggat gccggcgccg
     2821 atccagcggg tggtccaggc caccagcgcc agcaccagta ccaggccgat ggcctcggcc
     2881 cacaggcggg tgcgggcagc ggcggtttcc gcctccaatt ccacccgcat ctgctggtcc
     2941 agatccacca ccagcccggc cagggccgcg attcgccggg acgccacttc aaaccacatc
     3001 gccggggtcg gcccctggat cgcgtcgagc tgcgactcct gctgctgcaa gcggcgccgg
     3061 gtgtcataga agccgctcag gctactgtcc ccggtaacgg cacggtaccg ctccagaaaa
     3121 ccagggtccg ccagccgcgc cagcatgcgc tcca
//
LOCUS       DASUEG010000487         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_579200, whole genome shotgun sequence.
ACCESSION   DASUEG010000487 DASUEG010000000
VERSION     DASUEG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579200"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="VFX11_09095"
     CDS             <1..2241
                     /locus_tag="VFX11_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HEX4938818.1"
                     /translation="GTMTSVQDFPGRTGYWHIGVPPSGPFDSLSFRLGNRLLGNDERA
                     AGLEITLNGPTLRFNCDTHIALTGATMAATLDGTALKVWQRYAVKAGQTLYLGKVQQA
                     GARSYLCVAGGFQCEDYLGSKSTFTLGQFGGHGGRALRTGDVLHLAAPLQNTDINTAP
                     AKIAKDLQPAIGNEWQLHVIYGPHGAPDFFTPQDIDTFFAAEWEVHYNSSRTGIRLVG
                     PKPQWARTDGGEAGMHPSNIHDNAYAIGTIDFTGDMPVILGPDGPSLGGFVCPATVIT
                     ADLWKLGQLKAGDKVRFIPVDMNTANTLERNQLQAIASLEAITTEITPAHPTTPIIKT
                     IAASAHQEQVVYRPSGDHYLLVEYGPLQLDIRLRFRVHALMLWLQGQNIAGMRELTPG
                     IRSLQVHYDPLQLPLQQLLDILTSAEQQLQNIDTLEVPSRVVHLPLSWDDAACRLAIE
                     KYMQSVRKDAPWCPSNIEFIRRINGLDSIDDVKKIVFDAHYVVMGLGDVYLGAPVATP
                     LDPRHRLVTTKYNPARTWTAENSVGIGGSYLCVYGMEGPGGYQFVGRTLQMWNRFKLT
                     DAFTQPWLLRFFDQIRFYEVSAEELVQIRRDFPNGRYPIKIEETRFSLKQYEDSLQQN
                     RASIDAFTARRDQAFSEELQRWVESGQINFESAQDLAKDNADEQALPANCMAIESHVA
                     GNIWKILVQPGETIKAGQTVCILESMKMEIEITAPAGGTLYAVCRSEGQQVSAGQSLL
                     IIEENA"
     gene            2285..4108
                     /gene="atzF"
                     /locus_tag="VFX11_09100"
     CDS             2285..4108
                     /gene="atzF"
                     /locus_tag="VFX11_09100"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEX4938819.1"
                     /translation="MLPLNRIDMTIPGLLQHYRNGDFTPAQLLQTLQAEFARDTSNPV
                     WIHALSAQEVEPYLHNLDGKSADQLPLYGIPFAIKDNIDLAGIPTTAACPAFAYTPQK
                     SAHVVELLVAAGAIPLGKTNLDQFATGLVGVRSPEPWGPCHNAFNADYISGGSSSGSA
                     LAVAKGWASFALGTDTAGSGRVPAALNNIVGLKPSKGLLSTQGLVPACRSLDCISIFA
                     LTCADANRVFDIAARFDASDAFSRANPYSNGKRYFGPPATMPVVGVPATSELEFFGDA
                     DAQGCFTRSIDHLRQLGWNIREISIAPFVAAARLLYQGPSVAERTLVTQALLEQNPAA
                     LLPVIRRIVEAGLELSALDSFRAQYQLQQLIQQAQPLLQSVDAIITPTVGTTYRVDAV
                     QADPIQLNSNLGYYNNFMNLMDLAAVTVPTGFFRHGCGFGVTLFHRAFEDKRLLALAA
                     QLHAVAAPTLGATALKNPPNTFSAKGPQPFIPVVVCGAHLEGLALHWQLAERGAHKIE
                     STTTAPRYRMYALAGGPPFRPGLMREANGVALPVEVWAVPREQFGSFVADIAAPLGIG
                     KVELADGRWESGFICEPYGLQGAEDITDLGGWKPYLARGPR"
BASE COUNT          923 a         1441 c         1273 g          801 t
ORIGIN      
        1 ggcacaatga ccagcgtgca ggattttccc ggtcgcaccg gttactggca tattggcgtg
       61 ccaccctccg gtccgttcga cagcctgtcg ttccgcctcg gcaaccgcct gctcggaaat
      121 gacgagcggg cagcaggcct ggaaatcacg ctcaacggcc ccaccttgcg cttcaactgc
      181 gacacgcaca ttgcacttac cggcgcgacc atggcggcca cgctggatgg cacggcgctg
      241 aaagtctggc aacggtatgc agtgaaagcc gggcagacgc tgtatctggg caaggtgcag
      301 caggccggcg cccgttccta tctgtgtgtc gcaggcggtt tccagtgcga ggattatctg
      361 ggttcaaaat ccacctttac cctgggccag tttggcggtc acggcggacg cgcgctgcgc
      421 accggcgatg tgctgcatct ggcggcgcca cttcaaaaca ctgacatcaa taccgcgcca
      481 gccaaaattg caaaagacct gcaaccggcc atcggcaacg aatggcagct gcacgttatt
      541 tacggacccc acggcgcgcc ggattttttc acgccgcagg atatcgacac tttcttcgcc
      601 gccgaatggg aagtgcatta caactccagc cgtaccggca ttcgcctggt aggcccgaag
      661 ccgcaatggg cgcgcaccga cggtggcgaa gccggcatgc acccgtccaa tatccacgac
      721 aatgcctacg ccatcggcac catcgatttc actggcgaca tgccggtaat tctggggccg
      781 gacggtccgt cgctgggcgg cttcgtctgc cctgccactg tgatcacggc agacctgtgg
      841 aaactcgggc agctgaaagc cggcgacaag gtgcgcttta ttccggtgga catgaacacc
      901 gccaacaccc tggaacgtaa ccagctgcag gccatcgcgt ccctggaagc cattaccact
      961 gaaatcacac cggcacatcc cactacaccc atcatcaaaa ccattgccgc cagcgcgcac
     1021 caggagcagg tggtgtaccg cccctccggc gatcactatc tgctggtgga atacggtccg
     1081 ctgcaactgg atatccgact gcgcttccgt gtgcacgcgc tgatgctgtg gctgcaaggc
     1141 cagaacattg ccggcatgcg cgaactcacc cctggtatcc gctcgctgca ggtgcattac
     1201 gatccgctgc aactgccgct gcagcagctg ctcgacattc tcaccagtgc cgaacagcag
     1261 ctgcagaata tcgacacgct ggaggtaccg tcgcgcgtcg tgcacctgcc gttgagctgg
     1321 gacgacgccg cctgccgtct ggccatcgaa aaatacatgc agtcggtgcg caaggatgcg
     1381 ccctggtgcc ccagcaacat cgagttcatc cgccgcatca acgggctgga ttccatcgac
     1441 gacgtgaaaa aaattgtttt cgacgcgcac tacgtggtga tgggtctggg cgatgtgtac
     1501 ctgggtgcgc cggtggccac accgctggac ccgcgtcatc ggctggtcac caccaagtac
     1561 aatccggccc gcacctggac ggcggaaaat tccgtcggca tcggcggttc ctacctgtgc
     1621 gtgtacggca tggaaggccc cggcggctat caatttgtgg gccgcaccct gcagatgtgg
     1681 aaccgattca aactgaccga cgcctttacc cagccctggc tgttgcgttt tttcgaccag
     1741 atccgctttt acgaagtgag cgccgaagaa ctggtgcaga tccggcgcga ttttcccaac
     1801 ggccgttacc cgatcaagat tgaagaaaca cgcttcagcc tgaagcagta cgaggattcc
     1861 ctgcaacaaa accgtgccag cattgacgcc ttcacggcca gacgcgatca ggctttcagc
     1921 gaggaactgc aacgctgggt ggagagcggg cagatcaatt tcgaatccgc ccaggatctg
     1981 gcgaaggata acgccgacga acaggccctg cccgccaatt gcatggccat cgaaagccac
     2041 gtggcgggca atatctggaa aattctggtg cagcctggcg aaaccatcaa ggccgggcag
     2101 actgtttgca ttctggaatc catgaaaatg gaaatcgaga tcacggcgcc ggccggtggc
     2161 accctgtacg cagtctgccg cagcgaaggc cagcaggtca gcgccggaca gagcctgctg
     2221 atcattgagg aaaatgcctg atgccaccaa gaatgacaac gccgctgcaa aagaggatca
     2281 ccgcatgctg ccactgaacc gcatcgacat gaccattccc ggtctgctgc agcattatcg
     2341 caacggcgat ttcacgccgg cgcagctgct gcaaaccctg caggcagaat tcgcgcgcga
     2401 cacctccaat ccggtgtgga ttcatgcact gtcggcgcag gaagtggaac cctacctgca
     2461 caatctggat ggcaaatccg ctgaccagtt gccgctgtac ggcattccgt tcgccatcaa
     2521 ggacaatatc gatctggcgg gcattcccac caccgccgcg tgcccggcat ttgcctacac
     2581 accacaaaaa tccgcgcatg tggtggaact gctggtggcc gccggcgcga ttccgctggg
     2641 caaaaccaat ctggaccagt tcgccaccgg actggtgggc gtgcgctcgc cggaaccctg
     2701 gggcccgtgc cacaacgcat tcaatgcgga ttatatttcc ggcggctcca gttccggctc
     2761 cgcgctggcg gtggcgaaag gctgggccag ttttgccctg ggcaccgaca ctgccggctc
     2821 cggccgcgtg cctgccgcgc tcaacaatat cgttggcctg aagccgtcga aaggattgct
     2881 gagcacgcag ggactggtgc ccgcctgccg cagcctggac tgcatcagca tctttgcgct
     2941 gacctgcgcc gacgccaacc gcgtgttcga catcgctgcc cgctttgatg ccagcgacgc
     3001 tttcagccgc gccaatccct attccaacgg caaacgttat ttcggaccgc ccgccaccat
     3061 gccggtggtg ggtgtgccag ccacatccga gctggaattt tttggcgatg ccgatgcgca
     3121 aggctgtttt acccgcagca tcgatcatct gcgtcaactg ggctggaaca tccgcgaaat
     3181 atccattgcg ccgttcgtgg cggcggcgcg tctgctgtac cagggcccct cggtagccga
     3241 gcgcacactg gtcacgcagg cattgctgga acagaatccc gccgcgctgc tgccggtgat
     3301 ccgccgcatc gtggaagccg gcctggaatt gtcggcactg gacagcttcc gcgcgcaata
     3361 ccagttgcag caactgattc agcaggcaca accgctgctt caatcggtgg acgccatcat
     3421 cacacccacc gtcggcacca cctaccgcgt cgacgccgtg caggccgatc cgatccagct
     3481 caattccaat ctgggctact acaacaattt catgaacctg atggatctgg cggcggtcac
     3541 cgtgcccacc ggatttttcc ggcacggctg cggtttcggc gtcaccctgt ttcaccgcgc
     3601 gttcgaggac aaacgcctgc tggcactggc ggcgcaactg cacgccgttg ccgcacccac
     3661 gctgggtgcc accgcattga aaaatccgcc caataccttc agcgccaaag gtccgcaacc
     3721 gttcattccc gtggtggtgt gcggcgccca tctggaaggg ctcgccctgc actggcagct
     3781 ggccgaacgc ggcgcgcaca aaatcgaatc caccaccacc gcaccgcgct accgcatgta
     3841 cgccctcgcc ggcgggccgc cgttccggcc gggtctgatg cgggaggcaa acggcgtcgc
     3901 attgccggtg gaagtctggg ccgtgccgcg cgagcagttc ggttccttcg tcgccgacat
     3961 cgccgcaccg ctgggcatcg gcaaggtgga actggcggac ggccgctggg aatccggttt
     4021 catctgcgaa ccctacggcc tgcagggggc ggaagacatc accgacctgg gcggctggaa
     4081 accgtacctg gcccggggcc cgcgctgaac ccgtgcctgc ccgacggcag gcactaatcc
     4141 ctatcgccaa ccatcacaaa gactcaaccc aactgaccaa aatgtattag acaaaatctg
     4201 cacacccgat ccatcattcc tgcaggcagt accttctcaa caagcagtac cttcggagcg
     4261 aacagttcag ggagcaggga ctatgagctg gctaagcaat cttctgggcg gcagcgcagg
     4321 caacatcatc gcggaagtgg ggaatgtcgc ggacaagttt cacttgagcg gcgaagaaaa
     4381 gcagcaattc aaactggaac tggaagcgct gctgcaaaaa cgcgacagcg aaatcgaa
//
LOCUS       DASUEG010000488         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_649079, whole genome shotgun sequence.
ACCESSION   DASUEG010000488 DASUEG010000000
VERSION     DASUEG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649079"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /gene="ilvN"
                     /locus_tag="VFX11_09105"
     CDS             <1..365
                     /gene="ilvN"
                     /locus_tag="VFX11_09105"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX4938820.1"
                     /translation="SRLTLTTTGNDRVIEQITKQLNKLVEVVKLVDLSEGAHIERELM
                     LIKLKATGAQRAEIKRTADIFRGQIVDVTSSVYTVQLAGTSDKLDAFIESVGEASILE
                     VVRTGVSGIARGDKVLAL"
     gene            441..1457
                     /gene="ilvC"
                     /locus_tag="VFX11_09110"
     CDS             441..1457
                     /gene="ilvC"
                     /locus_tag="VFX11_09110"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX4938821.1"
                     /translation="MKIYYDKDCDLSIIQSKKVAIIGYGSQGHAHANNLKDSGVDVVV
                     ALRPGSASAVKAEKSGLSVKAVPEAVKWADVIMILTPDEFQSQLYRNEIEPNIRPGST
                     LAFAHGFAIHYNQVVPRKDLDVIMVAPKAPGHTVRSEFVKGGGIPDLIAIFQNASGNA
                     KNLALSYASAIGGGRTGIIETTFKDETETDLFGEQAVLCGGAVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINAESRAAMRN
                     ALKRIQNGEYAKMFIAEGAHNYPSMTAYRRNNAAHPIEQVGAKLREMMPWINANKIID
                     KSKN"
     gene            1640..2449
                     /gene="pssA"
                     /locus_tag="VFX11_09115"
     CDS             1640..2449
                     /gene="pssA"
                     /locus_tag="VFX11_09115"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX4938822.1"
                     /translation="MRDAKDKEEPREEAEQDDDGVTFEVVEGEIEGGKKVRRRGVYLL
                     PNLFTTGALFSGFYAIVAAMNGLFETAAIAIFISMIFDGLDGRVARMTNTQSEFGAQY
                     DSLSDCISFGIAPALVAYAWSLSTLGKVGWMVAFVYAACAALRLARFNVQIDTADKRY
                     FTGLPSPSAAAVVAGMVWLGTSLQIDGSGGVVSVAAAIITAFAAVMMVSNVRYNSFKE
                     LDFKGRVPFFVILVVVLAIAVIFSYPAGVLWGLFLVYALSGPLTSFRKPRP"
     gene            2501..>2921
                     /locus_tag="VFX11_09120"
     CDS             2501..>2921
                     /locus_tag="VFX11_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mononuclear molybdenum enzyme YedY"
                     /protein_id="HEX4938823.1"
                     /translation="MLIRIPRSSDPKASEITSESQYLSRRDWIRNTGMLGAGLALGSA
                     SGSLLAAPSTGWKSLLTDKLSSRGKIPDTTGETLTPFEDATRYNNFYEFGTGKSDPVE
                     NSGKFRVDPWTVSIEGAVSKPLKLNLEDLLKPHALEER"
BASE COUNT          648 a          867 c          804 g          602 t
ORIGIN      
        1 tgtcgcggct taccctcacc actaccggca acgaccgcgt gatcgagcag atcaccaaac
       61 agctcaataa gctggtggaa gtggtgaagc tggtggatct cagcgaaggc gcgcacatcg
      121 agcgcgaact gatgctgatc aagctcaagg ccaccggcgc ccagcgtgcc gaaatcaagc
      181 gtaccgccga tatcttccgt ggccagatcg tggatgtcac cagttcggtc tataccgtgc
      241 aactggccgg taccagcgac aagctggatg cgttcatcga gtccgtcggt gaagcctcca
      301 tcctggaagt ggtgcgcacc ggcgtgtccg gcatcgcccg cggcgacaag gttctggcgc
      361 tctgagccaa cctgcacttt tcaacgttat atacacgacg ctcaacccgg ctgatcggga
      421 tttcatatag aggaatgaac atgaaaattt actacgacaa agattgcgac ctttccatca
      481 tccagagcaa aaaggtggcg atcatcggct acggctcaca aggccatgcc cacgccaaca
      541 acctgaagga ttccggcgtc gatgttgtgg tggcactgcg tcccggttcg gcgtcggccg
      601 ttaaagcgga aaaatcaggt ctgtcggtga aagcggttcc cgaagcggtc aagtgggcgg
      661 acgtgatcat gatcctgacc ccggacgagt tccagtccca gctgtaccgc aacgaaatcg
      721 agcccaatat ccgtccgggc tccaccctgg cattcgctca cggtttcgcg atccactaca
      781 accaggttgt gccgcgcaag gatctggacg tgatcatggt ggcgcccaag gcacccggtc
      841 acaccgtgcg ttccgagttc gtgaaaggtg gcggtatccc tgatctgatc gcgatcttcc
      901 agaatgcatc cggcaatgcc aagaacctgg ccctttccta tgcatctgcg atcggtggcg
      961 gccgtaccgg cattatcgaa accaccttca aggatgaaac cgaaactgac ctgttcggcg
     1021 agcaggccgt tctgtgcggt ggtgcagtgg agctcgtaaa agccggtttt gaaaccctgg
     1081 tggaagcggg ctacgcaccg gaaatggcat acttcgagtg tctgcatgag ctcaaactga
     1141 tcgttgacct gatgtacgaa ggcggcatcg ccaacatgaa ctactccatt tccaacaatg
     1201 cagaatatgg cgaatatgtg acaggccccg aagtcatcaa cgcggaatcc cgtgcggcca
     1261 tgcgcaatgc actcaagcgc atccagaacg gtgaatacgc caagatgttc atcgccgaag
     1321 gcgcgcacaa ctatccgtcc atgaccgctt accgccgcaa caatgctgct catccgatcg
     1381 agcaggttgg cgccaagctg cgggaaatga tgccctggat caatgccaac aagattatcg
     1441 acaagtccaa gaactgattg gttatatcca tatgcgaaaa gcgcggctcc ggccgcgttt
     1501 tttgtttcca cggttgaaaa aaaaggtgta caggctcaaa gtagttccac aatcgtgcaa
     1561 cgcatcaggt cgactcatga ctgacaaaga acccaccaaa cacgaatcca aaaaagatcc
     1621 tgccgcagct gtcgtcaatt tgcgcgacgc caaggataag gaagagcccc gggaagaggc
     1681 cgagcaggac gacgacggcg tcaccttcga ggtggtcgag ggggaaatcg aaggcggcaa
     1741 gaaggtacgg cggcgcggtg tgtatctgct gcccaatctg ttcacaaccg gtgcgttgtt
     1801 ttcaggcttc tatgccatcg tcgccgccat gaacggactg tttgaaaccg cggccatcgc
     1861 cattttcatc tcgatgattt tcgatgggct cgacggccgc gttgcccgca tgaccaatac
     1921 gcagagtgaa ttcggcgcgc aatacgatag tctctccgac tgtatttcgt tcggcatcgc
     1981 gccagcgctc gtggcctatg cttggtcttt gtccaccctc ggcaaggtcg gctggatggt
     2041 ggccttcgtc tatgccgcat gcgcagccct gcgcctggcc cgtttcaatg tgcagatcga
     2101 tactgccgac aaacgctatt tcaccggtct gcccagtccg tccgctgctg ccgtggtggc
     2161 gggcatggtg tggcttggaa cttccctcca gatcgacggc agtggcggcg tagtttcggt
     2221 ggccgctgcc atcatcacgg cctttgctgc ggtcatgatg gtcagcaatg tgcgttacaa
     2281 cagcttcaag gagctggatt tcaaaggccg cgtgccgttc ttcgtgattc ttgtcgttgt
     2341 gctggcgatc gccgtgattt tctcctatcc cgccggcgtg ttgtggggtc tgttccttgt
     2401 ttacgccctc agcgggccac tcacctcttt tcgtaaaccc cggccgtaac aaaccggcac
     2461 ctcccgcgtc taaaccttac atcaagcgca ggaggtgccc atgctgatcc gaattccccg
     2521 ctcatccgac cccaaagcct cggaaatcac gtccgaaagt cagtacctgt cgcggcggga
     2581 ctggataagg aacaccggca tgctgggcgc tgggcttgct ctcggtagcg catcgggctc
     2641 gcttctggca gcgccctcga caggctggaa atcgctgttg accgacaagc tttcaagccg
     2701 cggcaaaatc cctgatacaa ccggcgaaac cctcacccca ttcgaggacg ccacgcgcta
     2761 caacaatttc tacgagtttg gcaccggcaa gtccgacccg gtcgaaaaca gcggcaaatt
     2821 ccgggtcgac ccctggactg tttccatcga aggagccgtc agcaaacctc tcaagctcaa
     2881 cctggaggat ctgctcaagc ctcacgcgct cgaagaacgc a
//
LOCUS       DASUEG010000489         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1169326, whole genome shotgun sequence.
ACCESSION   DASUEG010000489 DASUEG010000000
VERSION     DASUEG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169326"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFX11_09125"
     CDS             <1..556
                     /locus_tag="VFX11_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938824.1"
                     /translation="QEQRPRSASQGEQQGARRDSEGRDAGQNSGRGQQRRNEQGGQRR
                     SNNTGERAAGGRGQGQAQGQGQRDGRSKAQGAGQGNGQRNSQQRTGQQRNQSRAVNAL
                     DSSPMGRRVDPSAEHRRAQAEGNRAPRPQGEGKRREGARDDNHGNRRSGGQGQAREGQ
                     AAARAADAPPRKNLVSRIKDLARR"
     gene            744..1682
                     /locus_tag="VFX11_09130"
     CDS             744..1682
                     /locus_tag="VFX11_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938825.1"
                     /translation="MRFEVKISLTLLIAMATTVAPAADFTLLDKKLQERTDAVLAMMG
                     YSVVPDLTTSTLSISNTDTGNPQLTMSQLAGGFTISRDVPLYLEGGAAYSRYDPVFAV
                     TNGSETRELPLKWTSMMLTMGIGWDFPLTDKLKLRPIANFAYGHLESDLSLAGRFLDY
                     QTGEDVNFLDGGKLDSVGYGGSMMLDYEDYLPEREIDVEWRYSYVRLDTYESTDDFVE
                     GSSDAISTSLWTRWRAPTPWQVMERPLRYVLEAAHSTFFGDQGELGFDQLSSLGIGIE
                     FDSSAYDMIITRTRLVFRYRFGEDVEGTAVGLAVSF"
     gene            complement(1695..>2160)
                     /locus_tag="VFX11_09135"
     CDS             complement(1695..>2160)
                     /locus_tag="VFX11_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938826.1"
                     /translation="GVLLDWHPFGGGFRVSAGGFNVDNELSGVGSGVGTYHIGDTTYT
                     VDENDDLEVNALIELGDGFKPYLGLGWGNSPSNKGGLLLSFDVGILFQGSPSADLEVT
                     GTATDEGTGLTVDFSTDPTVQEEVRKEEQNLEDEVKDFDLYPVISAGIGWRF"
BASE COUNT          454 a          658 c          651 g          397 t
ORIGIN      
        1 tcaggaacag cgcccacgca gtgcgtcgca aggcgaacag caaggcgcgc gccgtgacag
       61 cgaaggccgt gatgccgggc agaacagcgg ccgtggccag caacgtcgca atgagcaggg
      121 cggtcagcgt cgcagcaaca acactggcga acgtgccgcc ggcggacgcg gccaaggcca
      181 ggctcagggc cagggtcaac gtgacggtcg cagcaaagcc cagggtgccg gtcagggcaa
      241 cggccaacgc aacagtcaac agcgcactgg ccagcagcgc aaccagtccc gcgcggtgaa
      301 cgcactggat tccagtccga tgggacgccg cgtcgacccc tccgccgaac accggcgggc
      361 acaggccgaa ggcaaccgcg caccgcgtcc gcagggcgaa ggcaagcggc gggaaggcgc
      421 ccgggatgac aaccatggca atcgtcgcag tggcggtcag ggccaggccc gtgaaggtca
      481 ggctgctgcc cgcgccgccg acgcgccacc gcgcaagaat ctggtgtcgc gcatcaagga
      541 tttggcgcgg cgctgatacg gacgccggaa acaaaaaagc cgggctaaaa aaacccggct
      601 ttttttatgc ctgcttggcg cctgccactg cggtaaccgc cggttccgca acaggtggtt
      661 cagtgccctt tccagcagaa ccggcaagat gcgatactgt tcgaataacc aatcttatca
      721 atggccaagg aaaatccggt tgtatgcgtt ttgaggtgaa gatctcgctg acgctgctga
      781 tcgcgatggc aacgaccgtc gctccggctg ctgactttac actgctcgac aaaaaactgc
      841 aggagcgcac cgacgccgta ctcgccatga tgggctattc ggtcgtgccg gatctcacca
      901 ccagcaccct gtcgatttcc aacaccgaca ccggcaatcc gcagttgaca atgagccagc
      961 tggccggtgg gttcaccatc agccgggacg tgccgcttta tctggaaggt ggcgcggcct
     1021 acagccgtta cgatcccgtg tttgctgtca ccaacggttc tgaaacccgc gagttgccgc
     1081 tgaaatggac cagcatgatg ctgaccatgg ggattggctg ggattttccg ctcaccgata
     1141 aactcaagct gcgccccatt gcgaacttcg cctatggcca tctggaaagt gacctctccc
     1201 tggcggggcg atttctggat taccagaccg gcgaggatgt gaatttcctc gatggcggca
     1261 agctggactc ggttggttat ggcggttcga tgatgctcga ttacgaagat tatctgcccg
     1321 aacgcgaaat cgatgtggaa tggcgctaca gctacgtgcg gctggatacc tacgaatcca
     1381 ccgacgactt cgtcgaaggt tcgagcgacg ccatctccac cagtctgtgg acgcgctggc
     1441 gggcgccgac gccctggcag gtaatggagc ggccgctgcg ctatgtgctg gaagcggcac
     1501 actccacgtt ttttggcgat cagggggagc tgggattcga ccagctcagc tcactgggca
     1561 ttggcatcga attcgattcc agcgcctacg acatgattat cacccgcacc cgtctggtgt
     1621 tccgttaccg ctttggtgaa gacgtcgagg gaacggcagt gggcttggca gtgagtttct
     1681 gaggtttcct gcggtcagaa ccgccagcca atgccagctg aaatcaccgg gtaaagatca
     1741 aagtccttca cttcgtcttc cagattctgt tcttccttgc ggacttcttc ctgcaccgtc
     1801 ggatcggtgg agaaatccac ggtgaggccg gtaccttcgt cagtcgcagt accggtcact
     1861 tccagatccg cactgggaga accctggaac aggatgccca catcgaatga cagcagcagc
     1921 ccacccttgt tggagggtga atttccccag cccagtccga gatagggttt gaagccgtca
     1981 cccagttcga tcagcgcgtt cacttccagg tcatcgtttt catcgacggt gtaggtggta
     2041 tcgccaatgt gatacgtgcc gacgccgctg ccgacaccgc tcaattcatt gtccacattg
     2101 aagccgccgg cgctgacacg gaagccacca ccgaacgggt gccagtccag cagcacgccg
//
LOCUS       DASUEG010000490         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_212688, whole genome shotgun sequence.
ACCESSION   DASUEG010000490 DASUEG010000000
VERSION     DASUEG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212688"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VFX11_09140"
     CDS             <1..1377
                     /locus_tag="VFX11_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4938827.1"
                     /translation="QLMVMQASGGVMTKEYIDGSPIRVLASGPAGGVIGSAHTGRAKQ
                     SPNLLCVDMGGTSYDMSIVLNGAAPATAGWNMHHRYLVGVPMVQVETLGAGGGSICQI
                     NAGEVQVGPRSAGSEPGPICYGRGGTEPTVTDALLMLGILSADAEFAGGSFSLQRAGV
                     VEAFQKLGDQMGYSAEDAAYDCWRVVNANMIQAIRRATAGKGIDPKEMVMLAYGGNGP
                     AFAAVHADALGITKVLVPKASPTFSALGTLVAKPTIDEERSCTCSVGSPDLDKLKSLW
                     QQLEARADKYFADANFTRDKVNFHYQVNMRYAGQNFAVSFEMYQCRGSQDYLFLNDAF
                     GEKALQAFNKRHMEEFGHIREMELPEIIGVRLVSSVDTAAPPVGAGFSAPSVTATPMK
                     IRRANLGDGYQETPVFDGDKLRPGHEITGPAVIEETFTTIVVYPGWKVCVDDSGDYEM
                     RKRQQSAS"
     gene            complement(1447..2505)
                     /locus_tag="VFX11_09145"
     CDS             complement(1447..2505)
                     /locus_tag="VFX11_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4938828.1"
                     /translation="MSDNSFFSLDGPLEVMPTERQMRIANLGAFPFLARNRGADPRRL
                     LERHDIDPFSLRDPDHYIDCRSYVNLLEDCSNLLNDPLFGLRLAQIQEPDVYGCVTAL
                     CRASATMREAVDCLIKFIPVTHSPAAMQELVVNDNIAMLRWGVRTDLGNNKQANYQAA
                     LLNLKLLRQLGGQDFKPSYVKLAVDTRHKDIDELERQLGCRFHGRTTENVIAFPAPLL
                     DKRIPSANRLLFKLLGGYLTRVKDAARTTLVDRVQDYIRSSLSEGNCTIERCAEKMDV
                     SVRTLQAKLSESQLSFSDILEEQRIALAQSYLKQYQVSLDDVAANLGYSEQSSFGRAF
                     KRWTGMTPKQFRQQAATN"
     gene            2907..>3637
                     /locus_tag="VFX11_09150"
     CDS             2907..>3637
                     /locus_tag="VFX11_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4938829.1"
                     /translation="MTCLHGIKVLELAESVAGEYCGKLLADFGADIVKIEKPGSGSPT
                     RHLGPFSGAESGKEHSGLFAYLNTGKRSVELDLDTAAARDALKTLLVHVDAVIDDHNA
                     PWLKKLGLDAESVQQAFPGLVVCSISNFGLEPPEDRLHAEDLNVFHSSGWGFHTPTGA
                     DPSQPPLQGAGRFLPSYEAGIEAALCMVAALYERADSNLGQFIEISKQEVLASRIDYV
                     LAQMVTGEMNVGTHRTAFDLSGPAG"
BASE COUNT          784 a         1004 c         1065 g          784 t
ORIGIN      
        1 cagctgatgg tgatgcaggc cagcggcggc gtcatgacca aggaatacat cgatggctcg
       61 cccattcgcg tgctggcgtc aggcccggcg ggtggcgtga ttggttctgc gcataccgga
      121 cgggccaagc aatcgcccaa cctgctgtgt gtcgacatgg gcggtacatc ctatgacatg
      181 tcgatcgtgc tcaatggcgc ggcgccggca accgccgggt ggaacatgca ccaccggtat
      241 ctggtcggtg tacccatggt tcaggtggaa accctcggag cgggcggtgg ttcgatctgc
      301 cagatcaacg caggggaagt gcaagtggga ccccgctcgg ctggatcaga accggggcca
      361 atctgttatg gccgaggtgg cactgaaccc acggttacgg atgcgctgct gatgctgggc
      421 atcctgtcgg cagatgccga atttgccggc ggcagtttct ctctgcagcg ggcaggagtt
      481 gtggaagcat tccagaaact gggcgatcag atgggctaca gtgccgaaga cgccgcctac
      541 gattgctggc gcgtagtcaa cgccaacatg attcaggcca tccgccgggc caccgccggc
      601 aaggggatcg atccaaagga aatggtgatg ctggcatacg gtggcaatgg tccggcgttt
      661 gccgccgttc atgccgatgc gctgggtatc acaaaagtat tggtgccgaa ggcttcaccc
      721 acgttttctg cgttgggcac cctggttgcc aagccgacga tcgacgagga acgctcctgc
      781 acctgctcgg tgggaagccc tgacctggat aaactgaagt cgctgtggca gcaactggaa
      841 gcgcgggctg ataaatattt tgccgatgcg aatttcaccc gggacaaagt gaactttcac
      901 tatcaggtca acatgcgcta cgcgggccag aatttcgcgg tcagcttcga aatgtaccag
      961 tgccgtggct cccaggatta tctgttcctg aacgatgcat ttggcgaaaa ggcgctgcag
     1021 gcattcaaca agcgccatat ggaagagttt ggccacattc gcgaaatgga gctgcccgag
     1081 attattggcg ttcgcctggt gtcttcagtg gatacggcag caccgccggt gggtgcaggt
     1141 ttcagcgcac cgtcagtgac ggcaacgccg atgaaaatcc ggcgcgccaa tctgggcgac
     1201 gggtatcagg agacgcctgt ttttgatggc gacaaacttc ggccgggcca cgagatcacc
     1261 ggccccgctg tcatcgagga aacttttacc accattgtgg tctatccggg atggaaagtg
     1321 tgtgttgacg attccggcga ctatgaaatg agaaaacgcc agcaatctgc gtcatgacaa
     1381 accgcccgtg gcaccctgcc ctgtcacggg cggcttgttt tcaatgacca gcttcaggcg
     1441 acagcatcag ttggtggcag cctgctggcg aaactgcttc ggcgtcattc cggtccagcg
     1501 tttgaatgcg cggccaaagc tggactgctc cgaatagccc agattcgccg ccacatcgtc
     1561 gagcgagacc tgatactgct tcagataact ctgcgccagt gcaatgcgct gctcctcaag
     1621 gatgtcggag aaactgagtt gtgactcgct cagttttgcc tgcaatgtcc gcaccgaaac
     1681 gtccattttc tcggcgcagc gctcgatggt gcaattgcct tcggacaggg aagaacggat
     1741 atagtcctgc acccggtcca ccagcgttgt acgcgccgca tcttttacgc gagtcaaata
     1801 gccgcccagc aatttgaaga ggagccggtt ggcactgggg atacgcttgt ccagcagagg
     1861 tgccgggaaa gcgatgacgt tttcagtggt cctgccatgg aagcgacagc caagctggcg
     1921 ctccagctcg tcaatgtcct tgtgccgggt atcgacggcg agtttcacgt agctgggttt
     1981 gaaatcctga ccacccagct ggcgcagcaa tttcagattg agcagcgcgg cctgatagtt
     2041 ggcctgcttg ttgttgccca gatccgtgcg cacgccccac cggagcatgg cgatattgtc
     2101 gttcaccacc agctcctgca tcgccgcggg agaatgggtc accggaatga atttgatcag
     2161 acagtcgacc gcttcccgca tcgtcgcgga ggcgcggcac agggccgtca cacagccata
     2221 aacatcaggc tcctgaattt gtgccagccg caatccaaac aggggatcat tcagcaagtt
     2281 gctgcagtct tccagcaggt ttacgtagga acgacagtca atatagtgat ccggatcgcg
     2341 caggctgaaa ggatcaatgt catgacgttc cagcagccgg cggggatcgg ctccccgatt
     2401 ccgggccaga aaaggaaagg caccaagatt ggcgatgcgc atttgtcgtt ccgtcggcat
     2461 tacctcaagc gggccgtcca gactgaaaaa actgttatcg ctcatgagcc atcacctctt
     2521 tttagttttt cttgtatttg tcctggtttg acctgcttcg ccgcgccaac tgttcattga
     2581 atcacgtgaa ctgtcaggaa cccgtttgcg ggctgtggaa acctgtaaat cctgatgttg
     2641 atcgaaagcg ggacccggca atccatcacg cccatacggt tcacgtgcac ttgcacccgg
     2701 gcccctttac agacatgcta ctgcacacca ctgttgtttt acaacagaga caggaccaat
     2761 cccgcctgcc gcgcggggtg ttctagcagg tcttggccta aatattcata tatcattgat
     2821 atttatgact atttttacga tatcgacatt aagtgaatcg gcataggcag ccaacaaata
     2881 accaataatt tttgaaggga tttggcatga cgtgcttgca cgggatcaag gtgctggaac
     2941 tggcggaaag cgtcgctggc gagtattgcg gcaaactgct tgcagacttt ggggccgata
     3001 tcgtcaagat cgagaagccg ggcagcggga gcccaacccg ccacctgggc cccttcagcg
     3061 gcgctgaatc cggaaaagaa cacagcggac tgtttgctta tctgaatacg ggcaaacgtt
     3121 cggtggaatt ggatctggac accgcggccg cacgcgatgc gctgaagacg ctgctggtgc
     3181 atgtggatgc agtgattgac gatcacaacg ccccatggtt gaagaagctg ggtctggatg
     3241 ctgaatccgt gcaacaggca tttcccggcc tggtggtttg ttcaatttca aatttcggcc
     3301 ttgaaccacc ggaagaccgg ctccatgccg aagatctcaa tgtttttcac agcagcggct
     3361 ggggcttcca tacgcccacc ggcgccgatc catcgcaacc gccgctgcaa ggcgcaggtc
     3421 gttttcttcc gagctatgaa gccggaattg aagccgcgct gtgcatggtt gcggcgctgt
     3481 atgagcgcgc ggattccaac ctgggtcagt ttattgaaat ttccaaacag gaagtgctgg
     3541 cgtcccggat cgactacgtt ctggcacaaa tggtcactgg cgaaatgaat gtcggcaccc
     3601 accgcactgc cttcgatctg tccggccccg ccggcat
//
LOCUS       DASUEG010000491         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1989093, whole genome shotgun sequence.
ACCESSION   DASUEG010000491 DASUEG010000000
VERSION     DASUEG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989093"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFX11_09155"
     CDS             <1..417
                     /locus_tag="VFX11_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4938830.1"
                     /translation="EDLQCTLPTHDPVVAALHERYLQQQLQKLGHTSTRYRVFTEIVR
                     RLKNGEPRRAEIAASIHVSERTLQRRLREENISFQELLNDARRELAQQYLADTRLTLA
                     EVADLLGFGDQSNFFRASKRWFGVSPGQYRSRFESA"
     gene            complement(431..1168)
                     /locus_tag="VFX11_09160"
     CDS             complement(431..1168)
                     /locus_tag="VFX11_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938831.1"
                     /translation="MHQFQFIFALAALLLAACAQQSPVPPLNLDVPLADVSAHFVTER
                     YHAGDAHDAHDGHSSAEGTAQSEWQFWRTANRIEMNIPARQTGEVWMRDGNAIFYQKV
                     FHADRKIVEYQSADLDALNVPVQWRTNELMIDPQVLQRLQVDDERWIDGHPALELSGT
                     VDGIEYEVVWRVDLNLPQQLRKQDADGNRETTRLLKAELRAQARLQQPDSSGYDIIDY
                     ADLGDRERDPFVIAIQNQLPGGHAHSH"
     gene            complement(1248..2825)
                     /locus_tag="VFX11_09165"
     CDS             complement(1248..2825)
                     /locus_tag="VFX11_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX4938832.1"
                     /translation="MVTQLKQRKSPLAAAIGLLLLAGNVHAQDAVPASADEWFKQGKQ
                     AVKAAEKLKHNERKARNVILFVGDGMGVSTVTAARILEGQLREADGEFNRLSFEEFPY
                     MAHSVTASANQQTSDSAPTATAMVAGIKTNDGAISVDQTINRTEPSAEVTAAKSVKTI
                     MEQAEERGLSTGVVSTARITHATPAVNYAHISNRDWENNANLPAGATVKDIAAQLIDF
                     PYGNGIEVALGGGRSNFMTSQSVDPEYPTRKGSRTDGRELTQEWVSQPKSAYVWNKAG
                     FDAIDPASTDRLLGLFERSHMKYEADRANDTAGEPSLADMTKKAIELLQKNKKGYYLM
                     VEGGRIDHAHHAGNAHRALTDTIALSEAVKMAQEMTNDKDTLIVVTADHSHVFTLAGY
                     PSRGNPILGKAAINGVELKDSLGLPYTTLGYANGPGFVGDGTRKVLNPLTEGYVAAEY
                     VGSAARPDLSGIDTTDPNYLQEAAVPMGSETHAGEDVAIYAAGPKAHLFHGTQEQHVI
                     YHVMSQALGFSGSHWGW"
     gene            2965..>3194
                     /locus_tag="VFX11_09170"
     CDS             2965..>3194
                     /locus_tag="VFX11_09170"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEX4938833.1"
                     /translation="MELLLVVLLLGVIAGGWWYLSDVRETALQAAQRHCTAMGVQFLD
                     GSVMPDGMRLIRNRSGALALLQCFRFEFTTSG"
BASE COUNT          561 a          963 c          986 g          684 t
ORIGIN      
        1 gaggacctgc aatgcacgct gcccacccac gatccggtgg tggcggccct gcacgaacgc
       61 tacctgcaac aacaactgca gaaactcggg cacacttcca cccgctaccg cgtgttcacc
      121 gagattgtgc gccggctgaa aaacggcgag ccccggcgcg cggaaatcgc cgccagcatc
      181 cacgtatcgg aacgcacgct gcaacgccgc ctgcgggaag aaaatatcag cttccaggaa
      241 ttgctgaacg atgcgcggcg cgagctggcg cagcagtacc tggccgatac ccggctgacg
      301 ctggcggaag tggcggacct gctgggcttc ggcgaccaga gcaatttctt ccgcgccagc
      361 aagcgctggt ttggcgtgtc accgggacaa taccgctcgc ggttcgaatc ggcctgaccg
      421 cgtcccttcc tcagtgtgaa tgtgcatgcc cgcccggcag ctggttctgg atggcgatga
      481 cgaacggatc gcgttcgcgg tcacccagat cggcgtagtc gataatgtcg tagccgctgc
      541 tgtccggttg ttgcagcctg gcctgcgccc gtaattccgc cttgagcagc cgggtggttt
      601 cgcgattgcc atccgcatcc tgcttgcgca gctgctgcgg cagattcagg tccacccgcc
      661 acaccacctc gtattcaata ccgtccaccg tgccggacag ttccagcgcc ggatggccat
      721 cgatccagcg ttcatcgtcc acctgcaatc gctgcagcac ctgcggatcg atcatcagtt
      781 cgttggtgcg ccactgcacc ggcacgttca gcgcatccag atcggcgctt tgatactcca
      841 cgatcttgcg gtcggcatga aacactttct ggtagaaaat ggcgttgcca tcccgcatcc
      901 agacctcacc ggtctgccgc gcgggaatgt tcatttcgat gcggttggcc gtgcgccaga
      961 attgccattc gctttgggcg gtaccctccg cgctggagtg accgtcatgc gcgtcatgtg
     1021 catcgcctgc atggtagcgc tctgtcacga agtgcgcgct gacatccgcc agcggcacgt
     1081 ccagattcag cggtggtacc ggggattgtt gcgcgcaggc ggccagcagc agagccgcca
     1141 gtgcgaaaat aaattggaat tgatgcatcg ttcgttacct gtttgttctg caacggcagc
     1201 aggagcacgt ctgcacgcgc ccccgcccgt tgtgtttcca ataatgctta ccagccccag
     1261 tgcgaaccgg aaaagcccag cgcctgcgac atgacgtgat agatcacgtg ctgctcctgc
     1321 gtgccatgga acaggtgagc cttcggtcct gcggcataga tggcaacgtc ttcaccagcg
     1381 tgggtttccg aacccatcgg cacggccgcc tcctgcaggt aattcggatc ggtggtatcg
     1441 atgccgctca gatcaggacg cgcagccgat cccacgtact ccgccgccac atagccttcg
     1501 gtcagcgggt tcagaacctt gcgggtgccg tcgccaacga agcccgggcc attggcgtaa
     1561 cccagcgtgg tgtagggcag gccgagagaa tccttcagct cgacgccatt gatggccgcc
     1621 ttgcccagaa tcggattgcc acgggacgga taacccgcca gcgtgaacac atggctgtgg
     1681 tcggcggtga ccacgatcag ggtgtccttg tcgttcgtca tttcctgcgc cattttcacc
     1741 gcttcggaca gcgcgatggt atcggtcagc gcgcggtggg cgttaccggc gtggtgagcg
     1801 tggtcgatgc ggccaccttc caccatcagg tagtagccct ttttgttttt ctgcaacagc
     1861 tcgatggctt ttttcgtcat gtcggccagc gatggttcac cggcagtatc attggcgcgg
     1921 tctgcttcgt atttcatgtg cgaacgttcg aacagaccca gcaggcgatc agtgctggcc
     1981 ggatcgatgg cgtcgaagcc ggccttgttc cacacatacg cggacttcgg ctggctgacc
     2041 cattcctgcg tcagttcacg gccgtcggtg cggctgccct tgcgggtggg atattccgga
     2101 tcgacgctct ggctggtcat gaaattgctg cgacccccgc ccagcgccac ttcgatgcca
     2161 ttgccgtagg gaaaatcgat cagctgcgcg gcgatatcct tcaccgtggc accggcgggc
     2221 aggttggcgt tgttttccca atcgcggttg ctgatgtggg catagttcac cgccggcgtc
     2281 gcgtgggtaa tgcgcgcggt ggaaacaacg ccggtgctca gaccacgctc ttcggcctgt
     2341 tccatgatgg ttttgacgct tttcgctgct gtgacttccg cgctgggctc ggtgcggttg
     2401 atggtctggt caacggagat ggcaccgtcg ttggtcttga tgccggccac catcgccgtg
     2461 gcggtgggcg cggaatccga ggtctgctgg ttggcgctgg cggtgaccga gtgcgccatg
     2521 tacgggaatt cttcaaagct caggcggttg aactcgccat cggcttcgcg cagctggcct
     2581 tccaggatgc gggcagccgt gacggtggaa acacccatgc cgtcaccgac gaacaggatc
     2641 acgttgcgcg ccttgcgctc gttgtgcttc agtttttccg ccgccttcac ggcctgcttg
     2701 ccctgcttga accattcatc ggcgctggcg ggtacggcgt cctgcgcgtg tacattgcct
     2761 gccagcagca acagtccgat ggctgcggcc agcggcgatt tgcgttgttt caattgtgtg
     2821 accatggtga acaccccaaa actgttgaat aggaaatatg caggcaaaac agtagcgatg
     2881 ctgtgtgaca gtcaggtgaa cagcacgacc aggccgtgca ttgggttatg atgacgggca
     2941 taaaacgcag gtggacggaa caacgtggaa ctgttactag tggttttgct gctgggtgta
     3001 atcgccggtg gctggtggta cttgtcggat gtgcgtgaaa ccgcgttgca ggcggcacag
     3061 aggcattgca cggcgatggg tgtgcagttc ctggatggca gcgtgatgcc ggatggcatg
     3121 cgcctgatcc gcaaccgcag cggcgcactg gcattgttgc agtgcttccg ctttgaattc
     3181 accaccagtg gcga
//
LOCUS       DASUEG010000492         5292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_558359, whole genome shotgun sequence.
ACCESSION   DASUEG010000492 DASUEG010000000
VERSION     DASUEG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558359"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2497
                     /locus_tag="VFX11_09175"
     CDS             <1..2497
                     /locus_tag="VFX11_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX4938834.1"
                     /translation="DAQNPQSDLVYKGVVYNEMKGAMSSATSVLYEQLGRYLFPTTTY
                     HYNSGGDPESIPDLSYEQLKAFYDTHYHPSNAVFMTYGDIPAQDHQKAIHERALHRFQ
                     KLNRHITVPNEKRYPAPLKVETAFSHLPEEDETAQEAIDNKTHIVLGWLLGHSTDLES
                     QLKAHLMNDVLLDNSAAPLRKLLETGNLGAAPSPLCGLEDSNREMAFMCGIEGSSPEK
                     AEELERRVLEVLQDVADNGVPQARLEAALHQLELSQREIGGDHYPYGLQLLMSAVSTA
                     IHRGDPVQLLNLDPVLTKLHEDIKQPGYIQTLVKEWLLDNPHRVRIVMKPDDKLEENR
                     EQQLKRELANVKAALDDKQKQQLVEQAAALQARQQREEDESILPKVGLEDVPAQTPMP
                     PFTVKELPSGKHHLYVRGTNGLVYQQVFYDLPTLRDDQLQLLPLFSHFVGDLGAGQDD
                     YLTIQERITAVSGGVHGFSVMRGLIDNEQAVKGLMGFSGKALKRNSAALADLMHQIIE
                     QPRYDERDRLRELVAQQRARLDQSITGQGHSLAMVAASSGMNPAARISHQVGGLAGIR
                     AIKHLDNALSDTSKLGELQSQLEAMATQLKTGQRRFLIVGEQDITDSTAQHLDALWQS
                     PHGQDYAALALPEVREQVRQAWLTNTQVNFCAKAYPTVPADHPDAAVLTVLGGFLRNG
                     FLHRAIREQGGAYGGGATQESNIAAFRFYSYRDPRLNETLQDFDASVEWLLSREHGYQ
                     PLEEAILGVIGSLDKPASPAGEAKQAFQNRLFGRDDEFQKRFRERVLSTTVADLQRVA
                     QTYLQPEKASAAVISHKAAWDAAGLEHFELNQV"
     gene            2504..2905
                     /locus_tag="VFX11_09180"
     CDS             2504..2905
                     /locus_tag="VFX11_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938835.1"
                     /translation="MKTLHLRVLVGLLLLTLFSQTLLAATRMVHCAFPGNDTAVAVMQ
                     EPSPASPHCHTEEPAAAPHATMHQSMDDSQHSSHCQPDCSCCWNACSASLLVNSRIAL
                     ALIPATETYHSLSALLPASPVEQSLRPPRFS"
     gene            3053..4870
                     /locus_tag="VFX11_09185"
     CDS             3053..4870
                     /locus_tag="VFX11_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEX4938836.1"
                     /translation="MKNAFNINRNGLTRRQFVLGTAAAGTLLALGHPRILRAAPADAM
                     QTLRGNQFDLNLNYTQVNFTGQPATATTVNGGLPGPVLRWKQGDTVTLRVTNQLAQHS
                     SVHWHGIILPTEMDGVPGMSFDGIHPGQTFEYRFKVQQSGTYWYHSHSHMQEQTGLYG
                     AIVIDPAESDPVQADRDYVVMLSDWSDDDPEDVHARLKKMSHYYNFRERTAGDLWRDI
                     RDKGVAQTWNERAMWNQMRMSENDISDVNGYAYTFLMNGQTPAQGWNALFKRGEKVRL
                     RFINAAAMTFFDVRIPGLKMTVVAADGQLIDPVTVDEFRMGVAETYDVIVEPKEDMAY
                     CIFAQSIDRTGFARGNLTPDLSLQAEIPAMDPVPVLGHGDMGMNMDMSHGEHAGHDMA
                     GMDHSNMDHSNMDHGSMNHAMPPRPKMGSGKAGFGSAAAIVHAKTESGPQVDMIADAP
                     QYKLDDPGIGLRNHRKDFGRRVLTYYDLCNYYPTPDPREPGREIQLHLTGNMHRYLWS
                     FDGIPFSEAEPIHLKYGERVRFTLVNDTMMNHPIHLHGMWSDLETGDARYIPRKHTVI
                     VQPGSMISYLVTADAKGRWAYHCHLLYHMMGMMREVRVS"
     gene            4883..>5292
                     /locus_tag="VFX11_09190"
     CDS             4883..>5292
                     /locus_tag="VFX11_09190"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX4938837.1"
                     /translation="MKTNLKCLPVMALMTATLLAGNGASAMEEHRWLTGINLDQLELR
                     DTDDGTVTAWDARAWLGDDLNRFILNSEGDYLHGDQEHDSETEEAELQLLYSRAVAAY
                     WDFQAGWRGDLQPDPERHWLALGLQGLAPYFVDV"
BASE COUNT         1175 a         1629 c         1565 g          923 t
ORIGIN      
        1 tgacgcgcaa aacccccagt cggatctggt gtacaagggc gtggtgtaca acgaaatgaa
       61 aggtgccatg agttccgcca ccagcgtgct gtacgagcag ctgggccgct atctgttccc
      121 caccaccact taccactaca acagcggcgg cgatcccgaa tcgattcccg atctgtcgta
      181 cgagcagctg aaagcgtttt acgacacgca ctatcatccc agcaacgccg tgttcatgac
      241 ctacggtgac attcccgcgc aggaccacca gaaagccatt cacgaacgcg cgctgcaccg
      301 gttccagaag ctgaaccgtc atatcaccgt acccaatgaa aaacgctatc ccgcgccgct
      361 gaaagtggaa accgcgttca gccatctgcc ggaggaagac gaaaccgcgc aggaggcaat
      421 cgacaacaaa acccatatcg tgctgggctg gctgctgggc cacagcaccg atctggaaag
      481 ccagctcaag gcccatctga tgaacgatgt gctgctggac aacagcgccg caccgctgcg
      541 caagctgctg gaaaccggca acctgggcgc ggccccttcg cccttgtgcg gactggagga
      601 ttccaaccgc gaaatggcct tcatgtgcgg catcgaaggc agctcgccgg aaaaagccga
      661 agaactggaa cggcgcgtac tggaagtgct gcaggacgtg gccgacaacg gcgtgccaca
      721 ggcgcggctg gaagcagcac tgcaccagct ggaactgagc cagcgcgaaa tcggcggcga
      781 tcattatccc tacggtctgc aactgctgat gtcggcggtg tccacggcca ttcatcgcgg
      841 cgatccggtg caactgctga atctcgatcc ggtgctgaca aaactgcacg aggacatcaa
      901 gcagcccggc tacatccaga cgctggtgaa ggaatggctg ctggacaacc cgcaccgtgt
      961 gcgcatcgtg atgaagccgg acgacaagct ggaggaaaac cgcgagcagc agctgaagcg
     1021 cgaattggcc aacgtgaaag ccgcgctgga cgacaaacag aaacagcagc tggtggaaca
     1081 ggccgctgca ttgcaggcgc gtcagcagcg tgaggaagac gaatccattc tgccaaaagt
     1141 cggactggaa gatgtgccgg cgcaaacgcc gatgccaccg ttcaccgtga aggagctccc
     1201 gtccggcaag caccatcttt atgtgcgcgg caccaacggc ttggtttacc agcaggtgtt
     1261 ttacgatctg cccacactgc gcgacgatca actgcagctg ctcccgctgt tcagccattt
     1321 tgtcggtgac cttggcgcgg gccaggatga ttatctgacc atccaggaac gcattaccgc
     1381 cgtgagtggt ggcgtacacg gcttcagcgt gatgcgcggc ctgatcgaca acgaacaggc
     1441 ggtaaaaggc ttgatgggct tttccggcaa ggcgctgaaa cgcaacagcg ccgcgctggc
     1501 cgacctgatg caccagatca tcgagcagcc ccgctacgac gagcgagatc gcctgcggga
     1561 actggtggcg cagcagcggg cgcggctgga ccagagcatt accggtcagg gtcactccct
     1621 ggcaatggtg gcggccagca gcggcatgaa cccggcggcg cggatttccc atcaggtggg
     1681 tggtctggcc ggaatccgtg ccatcaagca tctggataac gcactgtcgg ataccagcaa
     1741 actgggcgaa ctgcagtcgc agctggaagc gatggccacg caactgaaaa ccggccagcg
     1801 ccgcttcctg attgtgggcg agcaggacat taccgactcg accgcgcagc atctggatgc
     1861 cctctggcaa tcaccgcatg gccaggatta tgcggcgctg gctctgccgg aagtgcggga
     1921 gcaggtgcgc caggcgtggc tcaccaacac acaagtgaat ttctgtgcca aggcgtaccc
     1981 cacggtgccg gcggaccatc ccgacgcagc cgtactgacc gtgctgggcg gctttttgcg
     2041 gaacggcttt ttgcaccgcg ccattcgcga acagggtggc gcctacggtg gcggcgccac
     2101 gcaggaatcc aatatcgcag cgttccgttt ttattcctac cgcgatccgc gcctgaacga
     2161 aaccctgcag gatttcgacg cctcggtgga atggttgctg tcccgcgagc acggctacca
     2221 gccgctggaa gaagccattc tgggcgtgat cggatcgctg gacaaaccgg catccccggc
     2281 gggcgaagcc aagcaggcgt tccagaaccg cctgttcggc cgcgatgatg aattccagaa
     2341 gcgtttccgc gaacgggtgt tgtccaccac cgtggcggat ctgcagcggg tggcgcaaac
     2401 ctatctgcag ccggaaaaag ccagcgcggc agtgatcagc cacaaggcag cgtgggatgc
     2461 cgcgggcctg gaacatttcg aactgaatca ggtctgactc cagatgaaga cactgcacct
     2521 gcgcgtactg gttggtttgc tgctgctgac actgttcagc cagacgctgc tggccgcaac
     2581 gcgcatggtg cattgtgcat tccccggcaa cgacaccgcc gttgccgtca tgcaggaacc
     2641 atcgccagcc tctccgcatt gtcatactga agaacctgcc gcagcaccgc acgcgacaat
     2701 gcaccagtcg atggacgatt cgcaacattc cagccattgc cagccggatt gttcctgctg
     2761 ctggaatgcc tgttccgcca gcctgctggt caacagccgc atcgcactgg cgctgattcc
     2821 tgccactgaa acctaccatt ccctcagcgc cctgctgccg gcgtcgccag tggaacagtc
     2881 gctgcgcccg ccccgctttt cctgacgcag gcaagttgcg cccgcaatcg gagtgcaacc
     2941 cgccggcggc ttcagcctga ccacagatcc tttgattgct ggaatcggtt gtacgaccca
     3001 cagaggtcaa cgatttcaca tcctctatac atccaccgat ggattacgtg acatgaagaa
     3061 tgcttttaac atcaaccgca acggcctgac acgccgccag tttgtgctgg gcacagcggc
     3121 ggcaggcacg ctgctggcac tgggacatcc acgcatcctg cgagccgccc ccgcggatgc
     3181 catgcaaacc ctgcgcggca accagtttga cctgaacctg aattacaccc aggtgaattt
     3241 caccggccag ccggccaccg ccaccaccgt caatggcggc ctgcccggcc cggtcctgcg
     3301 ctggaaacag ggtgacaccg taaccctgcg cgtgaccaac cagctggcac agcacagctc
     3361 ggtgcactgg cacggcatca ttctgcccac cgaaatggac ggcgtgcccg gcatgagttt
     3421 cgacggcatt catccgggcc agaccttcga ataccgcttc aaggtgcagc agagcggcac
     3481 ctactggtat cacagccatt cccacatgca ggagcagacc gggctctatg gcgccatcgt
     3541 gatcgatcct gccgaatctg atccggtgca agccgaccgc gattacgtcg tgatgctgtc
     3601 ggactggagc gacgacgatc cggaagacgt gcacgcgcgc ctgaaaaaaa tgagccacta
     3661 ctacaatttc cgcgaacgca ctgccggcga tctgtggcgc gacatccgcg acaagggcgt
     3721 ggcgcaaacc tggaatgagc gcgccatgtg gaaccagatg cgcatgagcg aaaatgacat
     3781 ttccgacgtg aacgggtatg cctacacgtt tctgatgaac gggcagacac cggcgcaggg
     3841 ctggaatgca ttattcaaac gcggcgaaaa agtgcgtttg cgttttatca atgccgccgc
     3901 catgactttt ttcgatgtac gcattcccgg cctgaaaatg acggtggtgg ccgccgacgg
     3961 tcagttgatc gatccggtga cggtggatga attccgcatg ggcgtggcag aaacctacga
     4021 cgtgattgtg gaaccgaaag aggacatggc ctactgcatt ttcgcccagt cgattgaccg
     4081 caccggtttt gcgcggggca acctgacgcc ggatctgtcg ctgcaggcgg agattcctgc
     4141 catggacccg gtgccggtgc tggggcacgg cgacatgggc atgaacatgg acatgtcgca
     4201 cggcgaacat gccgggcacg acatggcggg catggaccac agcaacatgg accacagcaa
     4261 catggatcac ggcagcatga atcacgccat gccgccaaga ccgaaaatgg gcagtggcaa
     4321 agccgggttt ggcagcgctg ccgccattgt gcatgccaaa accgagtctg gtccgcaggt
     4381 ggacatgatc gccgacgcgc cgcaatacaa gctggatgat cccggtatcg gcctgcgcaa
     4441 tcaccggaag gatttcggcc gccgcgtact gacctactac gatctgtgca actactaccc
     4501 cacgccggac ccacgcgaac ccggccggga aatccagctg cacctgaccg gcaacatgca
     4561 ccgctatctg tggtcgttcg atggcatccc cttcagcgag gccgaaccga ttcacctgaa
     4621 atatggcgag cgtgtccgct tcaccctggt aaacgacacc atgatgaatc atccgatcca
     4681 tctgcacggc atgtggagcg atctggaaac cggcgatgcg cgctacattc cgcgcaaaca
     4741 caccgtcatt gtgcaacccg gcagcatgat cagttatctg gtcaccgccg atgcgaaagg
     4801 ccgctgggcc taccactgcc atctgctcta tcacatgatg ggcatgatgc gcgaagtgcg
     4861 ggtgagctga gggagcatgc aaatgaagac gaacctgaaa tgtctgcctg taatggcatt
     4921 gatgactgca accctgcttg ccggcaacgg cgcgtctgcg atggaggaac atcgctggct
     4981 gaccggtatc aatctggatc agctggaact gcgcgacact gacgatggta cggtcaccgc
     5041 atgggacgca cgggcctggc tgggggatga cctgaacaga ttcatcctca acagcgaagg
     5101 cgactatctt cacggtgatc aggaacacga cagcgaaacc gaagaagctg aactgcaact
     5161 gctgtacagc cgcgccgtgg cggcctactg ggattttcag gcgggctggc gtggagacct
     5221 gcaaccggac ccggaacggc actggctggc actgggtctg caaggactgg caccctactt
     5281 tgtcgatgtg aa
//
LOCUS       DASUEG010000493         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_577017, whole genome shotgun sequence.
ACCESSION   DASUEG010000493 DASUEG010000000
VERSION     DASUEG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577017"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..615)
                     /locus_tag="VFX11_09195"
     CDS             complement(34..615)
                     /locus_tag="VFX11_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938838.1"
                     /translation="MARPCEFDEDDVLDRAMQTFWLHGYDGTSLEDLLAATGIARQSL
                     YNRFGDKRALFIKALRRYHTSGGDKLKPLFDQVRPVQTAFRQLFETASSEDDDAKRMG
                     CFMINAAMEMARHDVEIADIVTRNQRAFEDLFVAALEAGKSRGELPADYDCRANGRFL
                     VGAFFGLKVLAKSDPSSPILGDMVKVTLNVLPA"
     gene            807..1373
                     /locus_tag="VFX11_09200"
     CDS             807..1373
                     /locus_tag="VFX11_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938839.1"
                     /translation="MAQKGEATRQRIIDAAASLMQAQGFHATGLNQIIQESGAPKGSL
                     YFHFPDGKESIAIAAMETSGRQLSDMLALLLAASPRLADALDTLWRFFMDALAGSDFR
                     KGCPIATLTLEAAGEFPGIQACSERIYQDWQQQISRKLQADGQPPDRADMLAGALLAL
                     LEGALVLARGKRSLVPLQQALQAAKVLI"
     gene            1450..>2062
                     /locus_tag="VFX11_09205"
     CDS             1450..>2062
                     /locus_tag="VFX11_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4938840.1"
                     /translation="MQPTTLNTSAHHPAAARPQTVLVTGATGFIGRWLLRELTAQGHH
                     VLALLRRPQTQQGELARWLDARGGDSARLQSVQGDLEADGWGLAPDDRARLQQVDAIF
                     HLASRFAWNLDAQLARRANVESLQTLLELVDANAGLQRLVWVGGYMVGNPRLQGTLAQ
                     LEQGRGWQGVYRRHGAYEASKLEGYVVLKQALARRTIPWTLVHP"
BASE COUNT          392 a          583 c          700 g          387 t
ORIGIN      
        1 cacggcaaag gacagggtgc aatggggggc ttgtcaggcg ggcagtacgt tgagggtgac
       61 cttgaccatg tcgccgagga tgggggagct ggggtcgctc ttggccagta ctttcaggcc
      121 gaagaaggcg cccaccagga agcgaccgtt ggcgcggcag tcgtagtctg ccggcagttc
      181 gccgcgggac ttgccggctt ccagtgcggc gacgaacaga tcctcgaacg cacgctggtt
      241 gcgggtgacg atatcggcga tttccacgtc gtggcgggcc atttccatgg ctgcattgat
      301 catgaagcag cccatgcgct tggcgtcgtc atcctcgctg ctggcggttt cgaacagctg
      361 gcggaaggca gtctgcaccg gccgcacctg gtcgaacagg ggtttgagct tgtcgccgcc
      421 actggtgtga taacggcgca gggccttgat gaacagggcg cgcttgtcgc caaagcggtt
      481 gtacaggctt tgccgggcga tgccggtggc ggccagcagg tcttccaggc tggtgccgtc
      541 gtaaccgtgc agccagaagg tctgcatggc gcggtcgagc acatcgtctt cgtcaaattc
      601 gcaagggcgg gccataaagg tggatcgtac tcggttctgg aatggacagt ctaatgatta
      661 tccctgaaca ggttttgggc ggcaagcggg accaggtgaa aggtcgggtg aaaggtcggg
      721 tgaaaggttg agcaagtgtt ggggttgaat aaatagaccg gtcgatatat tattaaaaaa
      781 tacggctcac caggaagaca acgatcatgg cccagaaagg cgaagcgacc cgccagcgca
      841 tcatcgacgc tgctgcttca ttgatgcagg cccagggctt tcatgccacc ggactgaacc
      901 agatcatcca ggaaagtggc gcccccaagg ggtcactgta ttttcatttt ccggacggca
      961 aggagtcgat tgccattgcg gcgatggaaa cctccgggcg ccagctgagt gacatgctgg
     1021 cactgctgct ggcggccagc ccccgcctgg cggatgcgct ggataccctg tggcggtttt
     1081 tcatggatgc gctggcagga tcggattttc gcaagggctg ccccatcgcc accctgaccc
     1141 tggaggcggc cggcgagttt cccgggattc aggcctgcag cgaacgcatt tatcaggact
     1201 ggcaacagca gatcagccgc aaactgcagg cggacggtca gccgccggat cgggccgaca
     1261 tgctggccgg tgcgctgctg gccttgctgg aaggggcgct ggtgctggcc cggggaaaac
     1321 gctcgctggt tccactgcag caggcgctgc aggcggcaaa agtcctgatc tgaaagggct
     1381 tttttgatga ctaaaaaata tatagaccgg tctactttta aagttcaaaa ctactctgga
     1441 gatagcctta tgcaaccgac aacgctgaat acatctgccc accacccggc ggctgccagg
     1501 ccgcaaaccg ttctggtcac cggcgccacc ggattcatcg ggcgctggct gctgcgggaa
     1561 ctgactgccc agggccacca cgtcctggcg ctgctgcgcc ggccgcagac acagcagggg
     1621 gaactggcgc gctggctgga cgcccggggc ggcgattctg cccgtctgca atcagttcag
     1681 ggtgatctgg aagccgacgg ttgggggctg gcaccggacg accgtgcccg tctgcagcag
     1741 gtggatgcca ttttccacct ggccagccgc ttcgcctgga atctggacgc gcagctggcc
     1801 cggcgggcca acgtggaatc gctgcaaacc ctgctggagc tggtggacgc caatgcgggc
     1861 ctgcagcggc tggtgtgggt gggcggctac atggtgggca acccgcgcct gcagggcacg
     1921 ctggcgcaat tggaacaggg ccggggctgg cagggcgttt accggcgtca cggcgcctat
     1981 gaggcgtcga aactggaagg ttacgtggtg ctgaaacaag ccctggcccg acgcactatt
     2041 ccctggaccc tggtgcatcc gt
//
LOCUS       DASUEG010000494         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_353733, whole genome shotgun sequence.
ACCESSION   DASUEG010000494 DASUEG010000000
VERSION     DASUEG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353733"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFX11_09210"
     CDS             <1..1161
                     /locus_tag="VFX11_09210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938841.1"
                     /translation="LLYIGATAFLQFTLHGFSFQYLWPESTSLNNSMILVLTGLMPFT
                     AIAFVWRFIGLTQLGTRLEKSALQLLYAGFGVVVVGAFTMHYMTVLKLAHTLSFLAVS
                     LGFYLGLKYWIKGVKAARIFALAWFVYLVFILLYLLDIKGIIQPNVISQHALEIGSAL
                     ELVLLSLAFADRLNTEKELRLQAQLKLNQDLDLLVRERTEELEHANRKLKEVSITDGL
                     TGLYNRRHFDDVFGSEYQRAFREKQPLAVIMIDLDHFKQINDAYGHPFGDLCLQRAAE
                     VIQNAIRRPPDLAARYGGEEFVVLLPNTDTAGASCVAETIRRNLEATVVNDGNHSVHL
                     TASLGAGAEIPPARQDLEKLLKRVDHCLYQAKHGGRNQVVAAAHGGPAALSA"
     gene            complement(1171..1890)
                     /gene="hda"
                     /locus_tag="VFX11_09215"
     CDS             complement(1171..1890)
                     /gene="hda"
                     /locus_tag="VFX11_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX4938842.1"
                     /translation="MSDRSHPDNLHPQLTLPLSGVPDFDFESFNASGNEQLLHRLHQI
                     ANGREPGWLFLSGAAGAGKSHLLQAVCLAAQQAGLHCLFVSGRELQSLHADVLQELSD
                     VELLCIDDLDRLLGQPAWEEQLFHLYNRLREQGGCLLLAASAPPRQLNIGLEDLRSRF
                     AAMELYAIQPLGDDDKRRVLQRAAQSRGFALADDVAAYILQRSSRDMVSLQKVLNRLD
                     QQSLREQRLITLPFVKKVMHW"
     gene            complement(1883..2431)
                     /locus_tag="VFX11_09220"
     CDS             complement(1883..2431)
                     /locus_tag="VFX11_09220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4938843.1"
                     /translation="MSYLRQLPNALTILRIILVVPFALCMMEREFAAALVILFVAGFT
                     DGLDGFLARYFQWRSRFGSIADPVADKILLITTYVALGALEQMPLWLVTVVVVRDLLI
                     FSCAIAYWFLVGKYDGKPTLLSKVCTFCLILVGLAVLANLVFLPLPPLFFVVYPWVLL
                     ALCVISLLQYLHMGLEGWRNRV"
     gene            complement(2450..>2830)
                     /locus_tag="VFX11_09225"
     CDS             complement(2450..>2830)
                     /locus_tag="VFX11_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938844.1"
                     /translation="ALHQLLDQVVDRMGAHYALVASSLGRQQLILNVEGVDDLQDFAA
                     LTQYLDSVLAISLHRMHALKADHAEFVLTLETGLDALRQVFRVDGKLVPSVVETPPIP
                     DGVAAPAPNEAPSGPVVIYYRWQG"
BASE COUNT          596 a          906 c          834 g          494 t
ORIGIN      
        1 ctgctgtaca tcggtgccac ggcgttcctg caattcaccc tgcacgggtt cagctttcaa
       61 tatctgtggc cggagagcac cagcctcaac aacagcatga tcctggtgct gacgggattg
      121 atgcccttca cggccatcgc cttcgtctgg cgtttcatcg gactgaccca gttgggcacc
      181 cgcctggaaa aatcggccct gcaactgctg tatgccggct tcggcgtcgt ggtggtaggc
      241 gccttcacga tgcactacat gaccgtgctc aagctggcgc acacactgtc gttcctggcg
      301 gtcagcctgg gtttttatct gggcttgaaa tactggatca agggcgtgaa ggcggcgcgc
      361 attttcgccc tggcctggtt tgtctacctg gtgttcatcc tgctctacct gctggacatc
      421 aagggcatca tccagccgaa tgtcatttcc cagcatgcgc tggaaatcgg ctccgcgctg
      481 gaactggtgc tgctgtcgct ggcgtttgcc gaccggctga ataccgagaa ggaattacgg
      541 ctgcaggcgc aattgaaact gaaccaggat ctggacctgc tggtgcggga acgcaccgag
      601 gagctggagc atgccaaccg gaagctgaag gaagtgagca tcaccgacgg tctcaccggc
      661 ctgtacaacc ggcgccattt cgacgacgtg tttggcagcg aataccagcg cgcattccgg
      721 gaaaagcagc cattagctgt catcatgatc gaccttgatc acttcaagca gatcaatgat
      781 gcctacggcc atccgttcgg cgacctgtgc ctgcagcggg cggcagaagt gattcaaaac
      841 gcgatccggc gcccaccgga tctggcggcg cgctatggcg gcgaggaatt cgtggtactg
      901 ctgccgaaca cggacacggc cggtgcttcc tgcgtggcgg aaaccattcg ccgcaacctg
      961 gaagccacgg tggtgaacga cggcaatcac agcgtgcatc tgacggccag cctgggcgcg
     1021 ggtgccgaga ttccgccggc acggcaggat ctggaaaagc tgttaaaacg ggtggaccat
     1081 tgcctgtacc aggccaagca tggcgggcgc aatcaggtgg tggccgctgc tcacggcggg
     1141 ccggcagccc tgtcggcctg agtgcaccgt ttaccagtgc atcaccttct tcacgaacgg
     1201 caacgtgatc aggcgctgct cccgcaaaga ttgctggtcc agccggttca gcactttctg
     1261 cagtgacacc atgtcacggc tggaccgctg caggatataa gccgccacat cgtctgccag
     1321 cgcaaaaccg cgactctggg ccgcccgctg cagcacccgg cgcttgtcgt cgtcccccag
     1381 gggctggatc gcataaagct ccatcgccgc aaagcgggac cgcagatctt ccagcccgat
     1441 attgagctgg cgcggtggcg ccgacgccgc cagcagcaga caaccgccct gctcccgcag
     1501 ccggttgtat agatgaaaca gctgttcctc ccaggcaggc tggcccagca gccggtccag
     1561 gtcatcgatg cagagcagtt ccacatccga caattcctgc aggacatcgg cgtgcagtga
     1621 ctgcagttcc cgccctgata cgaacagaca gtgcaggccg gcctgctgcg ccgccagaca
     1681 caccgcctgc agcagatgac tcttgcccgc ccctgcggca ccgctcagaa acagccagcc
     1741 cggttcgcgc ccgttcgcaa tctggtgcag ccggtgcagc agctgctcgt tgccggacgc
     1801 attgaaggat tcgaaatcga agtccggcac gccggacagg ggcagggtca gctgcggatg
     1861 caggttgtcg ggatgcgaac ggtcagacac ggttgcgcca tccctccagc cccatgtgca
     1921 ggtattgcag caggctgatc acgcacagcg ccagcagcac ccagggatac accacgaaaa
     1981 acagcggcgg cagcggcaga aacaccagat tcgccagcac cgccagcccc accaggatca
     2041 ggcagaaggt gcagaccttg ctgagcagcg tgggcttgcc atcgtatttt ccaacgagaa
     2101 accagtaagc gatggcacag ctgaaaatca gcagatcccg caccactacc accgtcacca
     2161 gccacagggg catctgctcc agcgcaccca gtgcgacata ggtggtaatc aacaggatct
     2221 tgtccgccac cggatcagcg atggaaccaa agcgcgagcg ccactggaaa tagcgggcca
     2281 gaaaaccgtc cagcccgtcg gtaaaacctg ccacgaacag aatgaccagt gcggcagcga
     2341 actcccgctc catcatgcac agcgcaaatg gcaccaccaa tatgatgcgc agtatggtga
     2401 gtgcattggg aagctgccgc agatacgaca tcagagccaa ccccgcgcgt cagccctgcc
     2461 agcggtaata aataacgact ggaccgctgg gcgcctcgtt cggggccggg gcggcgactc
     2521 catccgggat cggcggcgtc tccaccacgc tgggtaccag tttcccatcc acccggaaca
     2581 cctggcgcag ggcatccagg ccggtttcca gggtcagcac gaattcggca tgatccgcct
     2641 tcaaggcatg catgcgatgc aggctgatgg ccagtacgct gtccagatac tgggtgagtg
     2701 cggcgaaatc ctgcagatca tccacccctt ccacattcag gatcagctgc tgccgtccca
     2761 ggctggatgc caccagcgcg taatgggccc ccatgcggtc caccacctga tccagcaact
     2821 ggtgcagggc
//
LOCUS       DASUEG010000495         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1729791, whole genome shotgun sequence.
ACCESSION   DASUEG010000495 DASUEG010000000
VERSION     DASUEG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729791"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1735
                     /gene="zwf"
                     /locus_tag="VFX11_09230"
     CDS             260..1735
                     /gene="zwf"
                     /locus_tag="VFX11_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX4938845.1"
                     /translation="MCNLKASGNALFDFVLFGGRGDLALRKLLPALYHIYQSTPFHPS
                     TRIIGVGRAPLKREAYLSLVESRLQQHLEVESWDEVTWQQFAKHLHFASVDAQRGESY
                     RALREHLSDDGRPRVFYLSTHSSLYGAVAANLGAMNLISPASRVVLEKPIGRNLASAR
                     LISGQMSNYFTESQIYRIDHYLGKETVQNLLVLRFANPLFESLWNHHYVDHIQITIAE
                     SIGVEGRGDFYEETGALRDIVQNHLLQLLCIVAMEPPSSIDPDRIRDEKLKVLRSLRP
                     LQEASINSQVVRGQYTAGTSDSKKYIAYTEEEGVADSSTVETFVAMKVQIDNMRWSGV
                     PFYMRTGKRMPRRSCEIVVQFKEIPHWIFNTAKGPLMDNQLVITLQPNESITLRLCGK
                     RLGPGMDVRTVDLNLSPTSRNRRSPEAYERLLMDVVKGDQTLFLRQDELEEAWSWVDP
                     VLLHWERSSAPPESYTAGSWGPSGSTLLLAQDGRLWYEGSK"
     gene            1737..2429
                     /gene="pgl"
                     /locus_tag="VFX11_09235"
     CDS             1737..2429
                     /gene="pgl"
                     /locus_tag="VFX11_09235"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX4938846.1"
                     /translation="MYRLMESTCDADAATDCAEWLLALLETRRWEQQRISLFVSGGKS
                     PAAVFDLLATSDFSWQNVDLFLVDERWAPDQPQDQNATLVQQHLLTQNGAATRFQPLL
                     LDADFTRNLAACNEAVANIVQPDIVLLGMGLDGHTASLFPDAEDFSCAMEARNCYVAV
                     HPASAPYPRISMSFSWLVSARQIVLFIPGRDKRDVFNRIVKDTQSTSPIRPLVQQADT
                     RLTVITTRKRSS"
     gene            2426..4243
                     /gene="edd"
                     /locus_tag="VFX11_09240"
     CDS             2426..4243
                     /gene="edd"
                     /locus_tag="VFX11_09240"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX4938847.1"
                     /translation="MTHPVVLKIARRIRDRSQSSRDAYLQECALGATRPTSRAHLGCT
                     NLAHSYAAATPDEKILLRHTEITPNLGIVTAYNDMLSAHQPYGRYPDFLKSAALEFGA
                     TAQVAGGVPAMCDGVTQGQPGMELSLFSRDTIALSTAIALSHHVFDGALYLGICDKIV
                     PGLLIGALSFGFLPGLFVPSGPMHSGLSNEEKARVRVAYEEGKVGHDTLLQSEEASYH
                     SSGTCTFYGTANSNQMLLEMMGLQLPGSSFVPPHSELRQQLTRETVRILVEQSKKSGT
                     GIGELLEAENIVNAIIGLLTTGGSTNHTLHIPAIARAAGWIVDWNDFAELSDVIPLLA
                     RLYPNGAADVNHYHQQDGTPAVIKTLLQHGLLFPDIRTAYGLPFSSATHFPEPGDKAV
                     TWREARPPNPESTLLRPVEKPFSASSGLRVLSGNLGRSILKVSALPAHLPHVMQLPAR
                     VFHSQEELQAAFKTGELTGNFIAVLPGQGPSANGMPELHKLTPALTSLQNQGLQVALL
                     TDGRMSGASGKFPAAIHITPEAIHGGLIGKIRDGDLIELDWKTGRMELHVAAAELAQR
                     TDFILPPQTNTLGRRLFASARATVTAAEQGASFILRDLP"
     gene            4240..>4835
                     /gene="eda"
                     /locus_tag="VFX11_09245"
     CDS             4240..>4835
                     /gene="eda"
                     /locus_tag="VFX11_09245"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX4938848.1"
                     /translation="MSPFQQFLQHSKKVVPVISLPSLDMALPLMDALAAGGITVVEIT
                     LRTDCALDAITLLTRERPQLVVGAGTVKNAQQLQAVLSAGARFIVCPGLDLRLVEAAK
                     KASVPILPGIMTPTELMQAENAGLDTVKLFPATLAGGVEFISAMQAVFPAMKFFPTGG
                     IGEATLQDFLNCGNVVCVGGSWLTPAKLLQQQDWTKIR"
BASE COUNT          991 a         1480 c         1404 g          960 t
ORIGIN      
        1 gctggatttt ggtggcgggc aggaagcggg taccgtaacc ggccaccggg aagatacatt
       61 tcgtgatcat gggctaggct ctcagtaatt gcagtaaagg ctggagagcg cgaattctac
      121 acgttttgcc ggtggcgaat ccataattgg ctggctaatt gcagatcggt ccacaactgc
      181 cagattcagt gcatcagtta tactgaagtt caacggggat ttgattcagg acattgacag
      241 cgacaccgat agtcagggag tgtgcaactt gaaagcgtcg ggcaatgcgt tattcgactt
      301 tgtactgttc ggggggcgtg gggatctggc cctgcgcaag ttgctaccgg ccctctacca
      361 tatctaccag tcgacaccct ttcatccgtc cacccgcatc attggcgtgg gccgggcccc
      421 cctcaagcgc gaagcctatc tgagcctggt ggaaagccgc ctgcagcagc acctggaagt
      481 ggaaagctgg gacgaggtga cctggcaaca gtttgccaag catctgcact tcgcctctgt
      541 cgacgcgcag cggggcgagt cctatcgtgc actacgggag cacctgagcg acgatggccg
      601 gccgcgggtg ttttatcttt ctacccattc ttctttgtat ggcgccgttg ccgccaatct
      661 gggtgcgatg aatctgattt cgccagcgtc ccgggtcgtg ctggaaaaac ccatcggccg
      721 caatctggcg tccgccaggc tcatcagcgg gcagatgtcg aactacttca ccgaatccca
      781 gatctaccgt atcgaccatt atctgggcaa ggaaaccgtg cagaacctgc tggtgctgcg
      841 ctttgccaat cccctgttcg aatccctgtg gaatcaccat tacgtcgatc acatccagat
      901 cactatcgca gaatccatcg gcgttgaagg ccggggtgat ttttacgaag aaaccggcgc
      961 gttgcgcgac atcgtacaga accatctgct gcagttgctg tgtattgtcg cgatggagcc
     1021 tccgtcgagc atcgacccgg accggattcg cgatgaaaag ctgaaagtgc tgcgctcgct
     1081 gcggccattg caggaagcct cgatcaattc ccaggttgtt cgaggccaat acacagcggg
     1141 cacatccgac agcaaaaagt acatcgccta caccgaagag gaaggcgtgg ccgacagcag
     1201 cacagtggaa acctttgtcg ccatgaaagt gcagatcgac aacatgcgct ggtcaggcgt
     1261 gcccttctat atgcgaaccg gcaaacgcat gccgcgccgc agttgcgaga tcgtggtgca
     1321 gttcaaggaa atccctcact ggatattcaa taccgccaaa ggcccgctga tggataacca
     1381 gctggtgatc acgctgcagc ccaacgaatc catcacgctg cgtctgtgcg gcaagcgcct
     1441 gggtccgggc atggacgtgc gcaccgtcga tctgaatctg agccccactt cccgcaaccg
     1501 acgttcaccg gaagcctacg aacgcctgct gatggatgta gtgaagggcg atcagaccct
     1561 gttcctgcgg caggacgaac tggaagaagc ctggtcgtgg gtggacccgg ttctgctgca
     1621 ctgggaacgc tccagcgcac cgccggaatc ctacacggcc ggcagttggg gtccgtccgg
     1681 ctcaaccctt ttattggcgc aggacggacg tctctggtac gaaggatcaa aatgacatgt
     1741 accgtctgat ggaatcgacg tgcgatgcgg atgccgcaac cgactgcgct gaatggctgc
     1801 tcgccctgct ggagacccgg cgctgggagc agcagcggat cagccttttt gtatccggag
     1861 gcaagtcgcc ggcggctgtt tttgatttgc tggccaccag tgacttctcc tggcagaacg
     1921 tcgatctgtt tctggtggac gagcgctggg cgccggatca accccaggac cagaatgcca
     1981 ccctggtgca gcagcattta ctgacgcaga acggcgctgc aacgcgtttc cagccactgc
     2041 tgctggatgc cgatttcaca cgcaatctgg cagcctgcaa tgaagctgtt gcaaacattg
     2101 tgcagccgga tatcgtcctg ctggggatgg ggctggatgg tcacaccgca tccctgtttc
     2161 ccgatgcaga ggatttttcc tgcgcgatgg aagcgaggaa ttgctatgtg gccgtacatc
     2221 ctgccagcgc gccctacccg cgcatttcca tgagtttttc ctggctcgtg tcggcgcggc
     2281 agatcgtgct gttcattcct ggccgggaca agcgagacgt tttcaatcgg attgtgaaag
     2341 acacacaatc cacttcgccg atacggccgc tggttcagca ggcagacacg cggctgacgg
     2401 tcatcaccac ccggaagcgt tcatcatgac tcatcccgtc gtcctcaaga tcgcgaggag
     2461 aatccgcgac cgcagccaga gcagccgcga tgcctacctg caggaatgcg cattgggagc
     2521 gacccgtcct acttcgcggg cgcacctggg ctgcaccaac ctcgcccaca gttacgcagc
     2581 ggcgacgccc gacgagaaaa tcctgctgcg gcacacagag attacgccga atctcggcat
     2641 cgtcaccgca tacaacgaca tgctgtcggc gcatcagccc tacggacgtt atccggattt
     2701 cctcaaatcc gctgcgctgg aatttggcgc cacggcgcag gtggccggcg gtgtgccggc
     2761 catgtgcgac ggtgtgacac aagggcagcc cggcatggaa ctgtccctgt tcagccgcga
     2821 caccatcgca ctgtccaccg cgattgcgct gtctcatcat gtgttcgacg gcgcgttata
     2881 cctgggcatc tgcgacaaga tcgtgccggg gctgctgatc ggtgcgctgt cgttcggatt
     2941 tttgccggga ttgttcgtgc cctccggccc catgcacagc ggcttgtcga atgaggaaaa
     3001 ggcccgcgtg cgggttgcct acgaggaagg caaggtcggc catgatactt tgcttcaatc
     3061 ggaggaagcg tcgtaccatt cctctggcac ctgcaccttt tacggcaccg cgaattccaa
     3121 ccagatgctg ctggaaatga tggggctgca actgccgggc agttccttcg tgccgcccca
     3181 ttccgaattg cgccagcaac tgacccgcga aaccgtgcgc atcctggtgg agcaatcgaa
     3241 aaaatccggc acaggaattg gagaattgct ggaggcggaa aacatcgtca acgccatcat
     3301 cggcctgctg accaccggtg gttccaccaa tcacaccctg catattccgg ccattgcccg
     3361 tgctgccggg tggattgtgg actggaatga ttttgcggaa ttatcggacg tgattccgtt
     3421 gctggcgcgg ctgtatccga acggcgccgc cgacgtcaat cactaccacc agcaggacgg
     3481 cacgcccgct gtgatcaaaa ccctgctgca gcacggactg ctgtttccgg atattcgcac
     3541 cgcctacggt ctgcccttct ccagcgccac gcattttccc gaaccgggcg acaaggccgt
     3601 aacctggcgc gaggcccggc cgcccaatcc ggaaagcaca ttactgcgtc cggtggaaaa
     3661 accgttttca gccagcagcg gcctgcgcgt gttgagtgga aatctggggc ggtccatcct
     3721 caaggtctcg gccctgccgg cccatctgcc gcacgtcatg cagttaccag cgcgggtttt
     3781 tcacagtcag gaagagctgc aggccgcgtt caagaccggc gaactgacgg ggaatttcat
     3841 cgctgttctg cccggccagg ggccctccgc caatggcatg ccggaactgc acaaactcac
     3901 accggcgctg acgtcattgc agaatcaggg cctgcaagtg gcactgctga ccgatgggcg
     3961 catgtccggc gcctccggca aatttcccgc ggccattcac attacaccgg aagccatcca
     4021 cggcggactg atcggcaaaa tccgcgatgg cgacctgatc gaactcgact ggaaaaccgg
     4081 gcgcatggaa ttgcatgtgg cagccgcaga acttgcgcaa cgcaccgatt ttattctgcc
     4141 gccacaaacc aacacgctgg gccgccgcct gttcgccagc gcccgcgcca ccgtcaccgc
     4201 cgccgaacag ggcgccagtt tcattctgcg agacttgcca tgagcccgtt tcaacaattt
     4261 ctgcagcaca gcaagaaagt ggtgccggtc atttcgctgc cctcgctgga catggcactg
     4321 ccgctgatgg atgcactggc ggcgggcggt atcaccgttg tagaaatcac cctgcgcacc
     4381 gactgcgcgc tggacgccat caccctgctc acccgtgaac gtccacaact ggtggtgggc
     4441 gccggcaccg tgaaaaacgc gcaacaactg caggccgtgc tcagcgccgg cgcccgcttc
     4501 atcgtctgcc cgggcctgga tctgcgtctg gtggaagccg caaaaaaagc ctcagttccg
     4561 atcctgcccg gcatcatgac gccgaccgaa ctgatgcagg cggaaaatgc cggcctcgac
     4621 accgtaaagc tctttcccgc caccctggct ggcggcgtgg aattcatcag cgccatgcaa
     4681 gccgtttttc ccgcgatgaa atttttcccc accggcggca tcggcgaagc caccctgcag
     4741 gattttctga actgcggcaa tgtggtatgc gtggggggca gctggctgac gccggcaaaa
     4801 ctgttgcagc agcaggactg gacaaaaatc cggga
//
LOCUS       DASUEG010000496         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1730220, whole genome shotgun sequence.
ACCESSION   DASUEG010000496 DASUEG010000000
VERSION     DASUEG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730220"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFX11_09250"
     CDS             complement(<1..228)
                     /locus_tag="VFX11_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938849.1"
                     /translation="MNKRLVVPLLGTLFLSACNFVKLAPNAEDVLVLKPYQARECEQL
                     RRTTSQVMSKLWFVNRNEESMAEELTILAKNT"
     gene            302..1270
                     /locus_tag="VFX11_09255"
     CDS             302..1270
                     /locus_tag="VFX11_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4938850.1"
                     /translation="MKEHSKYRLIPDLLTRPAAAGMAGPIAVFSDWRSHCADLFQRWD
                     TPVDRAIAGGFDSMDTGIAFAFGYQAALQQMAPGHIDQRPACFCVTESGGNSPSAIQT
                     RLARVTPEQGFLQGEKTFVTNADQAELLLVAASLGDPVPGPDGKSRNPLVMVLVPAQS
                     PGVSITPLPALPFAPSVHHGIARFEQVPVAASQWLPGDGYSDYVKPFRTVEDIHVSAA
                     LLGFLLRIARAHRWPAASIEALLALIALHQWLAALPPKDSSTHLILAGARQQMEDWLQ
                     ANRPHWDLCPPELAQAWQRDSALLKVAGKARAQRTQTAWNTLQGAS"
     gene            1393..1872
                     /locus_tag="VFX11_09260"
     CDS             1393..1872
                     /locus_tag="VFX11_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4938851.1"
                     /translation="MFRTALSPVCPFRTARLGAAILLLSAITFAQSASADTPRPAKTE
                     FRFGLNQARPTATDRNILEQHGQFIAEHPEVRVTLHGHSDAQGNSLHNATLALKRAQA
                     VAQILREQGVALEQIEIVSWGAVVPAPRGQREADNRRVELDYNDEFLANVGLEFSDL"
     gene            complement(1888..2850)
                     /locus_tag="VFX11_09265"
     CDS             complement(1888..2850)
                     /locus_tag="VFX11_09265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4938852.1"
                     /translation="MEIIVEVLGRNGRVSERVKCRGQHVGIGRSYHNDVILADPYVCP
                     QHLELHYDAGTHHWKVQDISSQNGTFRVGRGRLTQPEVLQSGDEFDLGETRVRVLLPE
                     HEIAATRVLPSGRVLADYFALPVVAVALLIITLGVFAFARYLGQGTEVKLQELLLEAL
                     MFLAVPFVWACIWGMIGRVAVHDVRFGFHLSMGSLLVILVFAVSVLADFLAFGFSSEM
                     LALWLENGGEGLLLCGFLLASLWAATNLGRTARWILANSLAWGVIGVGLLAWMVNSDP
                     YETQGQEVYRLKPPFARLQPAVDLDTFMQSAEGLFTELDAEKSS"
     gene            complement(2838..>3465)
                     /locus_tag="VFX11_09270"
     CDS             complement(2838..>3465)
                     /locus_tag="VFX11_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938853.1"
                     /translation="SAQIATPEKPEWDAQLERQLLANQDQYLAELLDHDWKQEPLGDV
                     TVPTEIAPYVKCWGDKSDDKETLFQVTNRTCHVDETLFIGSYFTTGSFRLQYRWFETE
                     KLNALQFYTQMEDQFFLDINNNAYKDDVSPYACEDSFVDLNGKTWKVHWCARRYKRFP
                     ALHDVSVTLAALVSNRRGMLVNFSLAGVSRDNAMKITRRLLGAVQWKS"
BASE COUNT          695 a         1168 c          970 g          632 t
ORIGIN      
        1 cgtgtttttg gccaggatgg tcagctcctc cgccatgctt tcttcgttgc gattcacaaa
       61 ccagagcttg ctcatcacct ggctggtggt gcggcgcagc tgttcgcatt cccgtgcctg
      121 gtaaggcttc agtaccagca cgtcttctgc attgggcgcc agcttcacga aattacaggc
      181 tgacaaaaac agcgtgccaa gaagcggcac gaccaaacgc ttattcatag ttgactccta
      241 ttgcaatccg gtgcgcccgt ttgcatgctg acggggcccg ctaacaatgc cagaatttgc
      301 catgaaagaa cactcaaaat accgcctgat tcccgatttg ctgacccgtc ccgccgcggc
      361 gggaatggct ggcccgatcg ctgtcttttc tgactggcgc tcgcactgcg ccgacctgtt
      421 ccagcgctgg gacacccccg tcgaccgcgc catcgctggc ggcttcgaca gtatggacac
      481 cggcatcgca tttgcgttcg gttaccaggc cgcattgcag cagatggcac cgggacacat
      541 cgatcagcgt cccgcctgtt tctgcgtcac cgaaagcggc ggcaactcgc cgtccgccat
      601 ccagaccagg cttgcccgcg tcacaccgga acaggggttt ctgcagggcg aaaagacatt
      661 cgtcaccaac gccgaccagg ccgagctgct gctggtggca gccagtttgg gggacccagt
      721 gcctggccct gacggaaaat cccgcaatcc gctggtcatg gtattggtgc cggcgcagag
      781 tccgggcgtc agcatcaccc cgttgcccgc actgcccttt gcgccgtccg ttcatcacgg
      841 catcgcccgc ttcgaacagg tgcccgtggc ggcatcccag tggctgccgg gcgatggtta
      901 cagcgactac gtgaagccat tccgcaccgt ggaggacatt cacgtgagcg cggcactgct
      961 gggatttctg ctgcgcattg cccgcgccca tcgctggcct gctgccagta tcgaagcatt
     1021 gctggcactg atcgcactgc accagtggct ggcggcactg ccgcccaagg acagcagcac
     1081 ccatctgatt ctggcgggcg cgcggcaaca gatggaagac tggctgcagg ccaatcgccc
     1141 gcactgggat ctgtgtccgc cggaactggc ccaagcctgg cagcgcgaca gcgccctgct
     1201 gaaggtggca ggcaaggccc gtgcacaacg cacgcaaacc gcctggaata ccttgcaggg
     1261 cgccagctga ccctttgcca caattgatcc tgattgctcc cggattgcgc agcatgcaac
     1321 ccgggcgctg cccgaatccg ggcgcctact ggatgggacc gaatccccgg cccatcccgt
     1381 caggagccca tcatgtttcg caccgcgttg tcgcccgttt gcccgttccg cactgcccgc
     1441 ctgggggctg ccatcctgtt gctgtccgcc atcaccttcg cgcagagcgc cagcgccgac
     1501 accccgcgcc cggccaaaac cgaattcaga ttcggtttga atcaggcccg gcccaccgcc
     1561 accgatcgca acatcctcga acagcacggc cagttcatcg ccgagcatcc ggaagtgcgc
     1621 gtcacgctgc acggccacag cgatgcccag ggcaacagcc tgcacaacgc aactctggca
     1681 ctgaagcgcg cgcaggcagt cgcgcagatt ctgcgggaac aaggcgtggc actggagcag
     1741 atcgaaatcg tgagctgggg ggcggtggta cccgctcccc gtggtcagag agaagccgac
     1801 aaccgccgcg tcgagctgga ctacaacgat gaatttcttg ccaacgtcgg gctggagttc
     1861 agcgatctgt aacggacgcg cgacgaatca ggacgacttc tccgcatcca gttccgtgaa
     1921 cagcccttca gcagattgca tgaaggtgtc cagatccacc gccggctgca aacgggcgaa
     1981 cggcggtttc agccggtaca cctcctggcc ctgggtttca tagggatcgg aattcaccat
     2041 ccaggccagc aggcccacac cgatcacgcc ccaggccaga ctgttggcca gaatccagcg
     2101 cgccgtccgc cccagattgg tggcggccca cagactggcc agcaggaagc cgcacagcag
     2161 caggccctcg ccgccgtttt ccagccacaa cgccagcatt tcgctgctga aaccgaaggc
     2221 cagaaaatcc gccagcaccg acacggcgaa aaccagaatc accagcagac tgcccatcga
     2281 cagatgaaaa ccaaaccgta catcgtgcac cgccacccgt ccgatcatgc cccagatgca
     2341 ggcccacaca aatggcacag cgaggaacat cagcgcttcc agcaataatt cctgcagctt
     2401 cacctcggtg ccctgcccca ggtaccgggc gaacgcgaac acccccaggg tgatgatcag
     2461 caacgccacc gccaccaccg gcaatgcgaa ataatccgcc agcacgcgcc ccgacggcag
     2521 cacgcgggta gcggcgattt catgctccgg cagcaatacg cggacgcggg tttcccccag
     2581 atcgaattca tcgccagact gcaatacctc tggctgcgtc agacgcccgc gccccacccg
     2641 gaacgtgccg ttctgcgacg agatgtcctg cactttccag tggtgggtgc ctgcgtcata
     2701 gtgcaactcc agatgctgcg gacacacgta gggatccgcc agaatcacat cgttatggta
     2761 actgcggccg atgcccacat gctggccgcg acacttgacg cgctcgctca cgcggccatt
     2821 gcggccgagc acttccacta tgatttccat tgcaccgctc ccagcagacg gcgggtgatt
     2881 ttcatcgcgt tgtcgcggct tacaccggcc agggaaaaat tcaccagcat gccgcgccgg
     2941 ttcgacacca gtgccgccag cgtcaccgac acatcatgca gcgcgggaaa acgcttgtag
     3001 cgacgcgcac accagtgcac cttccaggtt ttgccgttca gatcgacaaa actgtcctcg
     3061 caggcgtagg gcgacacatc atccttgtag gcattgttat tgatgtcgag aaagaactgg
     3121 tcctccatct gcgtatagaa ctgcagcgca ttcagcttct cggtttcaaa ccagcggtac
     3181 tgcagacgga aactgccggt ggtgaaatag ctgccgatga acagcgtttc atccacatga
     3241 caggtacggt tggtgacctg gaacagggtt tccttgtcgt cgctcttgtc gccccagcat
     3301 tttacgtacg gcgcgatttc cgtcggcacg gtgacgtcac ccaggggttc ctgcttccag
     3361 tcgtgatcca gcaattccgc caggtactga tcctggttcg ccagcagttg ccgttccagc
     3421 tgcgcgtccc actccggttt ttccggcgtt gcaatctggg cggaa
//
LOCUS       DASUEG010000497        10128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1610499, whole genome shotgun sequence.
ACCESSION   DASUEG010000497 DASUEG010000000
VERSION     DASUEG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610499"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1738
                     /locus_tag="VFX11_09275"
                     /pseudo
     CDS             1..1738
                     /locus_tag="VFX11_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Tex family protein"
     gene            complement(1908..2756)
                     /locus_tag="VFX11_09280"
     CDS             complement(1908..2756)
                     /locus_tag="VFX11_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEX4938854.1"
                     /translation="MKAEENQTRIGQYNRLRVLRAVDFGVYLDGQELGDILLPRRYVP
                     AGCAVGDDVDVFVYVDSEDRLVATTDKPRAQVGECAYLKVVAVNAVGAFLDWGLPKDL
                     LVPFSEQYKPMEVGKSYVVHVFCDEETGRIAATSKLHRHLKEKTSSLKPQQPVSLLVC
                     GRTDLGYKAVIDNRFLGMLFNSDLLQPVRIGQKLKGYVKAIRDDGKIDLCLHLQTQAT
                     RDELADKILQHLQDNNGVSTLTDKSSPDDIYRQYRVSKASYKKAIGNLFKQKKIRIEQ
                     DRIVLV"
     gene            3114..3794
                     /locus_tag="VFX11_09285"
     CDS             3114..3794
                     /locus_tag="VFX11_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HEX4938855.1"
                     /translation="MQSPLRKCLPILAMTASTLFALPSAQAAFVMTLDDPNDSLSAVT
                     IYDGTGGDLNGVNGVITYSGSVGAFMVNVTTGVSKPLIGPAQLDLNSINVSGAAGTLL
                     VSISDTGFTGNFNALGASYGGTTQGAVDFDFLYDTANQQFGGSAFATDSFTSGGTHYA
                     FSNDLTGAVTPAALFSLTISTRITHDSAFEVSSFDAQIAPVPLPATIWLLGAAMAGLA
                     TLRRARRS"
     gene            3957..5534
                     /gene="gshA"
                     /locus_tag="VFX11_09290"
     CDS             3957..5534
                     /gene="gshA"
                     /locus_tag="VFX11_09290"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX4938856.1"
                     /translation="MTKTLLHRLESIRDTRLHHTLTTLKRGIEKESLRVTPNGTLSQA
                     RHPEALGSALTHQWITTDYSEALLEFITPAFQDLHRPLRFLNDVHRFTYQHIGEEKIW
                     VNSMPCVLGDELSIPIAEYGTSNSGRMKHVYRHGLWHRYGRYMQTIAGIHYNLSIPDN
                     FWQVYHQLQNSQQSLQDFISDQYFALIRNFQRNVGLAVYLYGASPAVCTSFLQGRNHK
                     LETLNKHTLFSRYGTSLRMSDLGYHNDAQSGLDISYNSLGEYVRSLQHAIRTPYSEYE
                     KIGVLKNGEYQQLNTNILQIENEFYSSIRPKRVSKRGERPTCALASRGVEYVELRCVD
                     LDPFTPMGINEGQMMFLDVFALYCLLEESPPIDEKEKTCNSQNLQAIVMHGRNPEMKL
                     ESKCVKMPFRQWAQQHLDNMLQVARLFDMAYDNDLHTKVVKQQMEKLRDPHLTPSSKV
                     IRQLDERDQSFFQFAMHCALEQEKHFRNNPLSPEETVPFLHEAKRSLEAQRRMEASDN
                     VSFEQYLESYFSPDVVC"
     gene            5749..5964
                     /locus_tag="VFX11_09295"
     CDS             5749..5964
                     /locus_tag="VFX11_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HEX4938857.1"
                     /translation="MTFKEIDNQNWSIETLNKAYRQGYMFGLSGENQGSCPYKSDVIA
                     AAWEAGWLDGRTQAEFVSSMESDCAIA"
     gene            6081..6578
                     /locus_tag="VFX11_09300"
     CDS             6081..6578
                     /locus_tag="VFX11_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX4938858.1"
                     /translation="MNLPSSRLCNAAGVAGSIVAMLFAYYYLQRTLGLDPCPLCLIDR
                     GLVMLAGFFFLLAFIHNPGRTGQRVYSGLALIPALLGVAVCWRHLWLESLPKHLVPEC
                     SPGLEYMLETFPIGETIRTIFNSAGECAEVKWRFLGLSIAGQTLLVFLGFTALSLLQI
                     LRKRD"
     gene            6966..7442
                     /gene="rsd"
                     /locus_tag="VFX11_09305"
     CDS             6966..7442
                     /gene="rsd"
                     /locus_tag="VFX11_09305"
                     /inference="COORDINATES: protein motif:HMM:NF008723.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HEX4938859.1"
                     /translation="MLTEATSPTDHWGSVDKLIKHWLKERQELIVLYCKVDGLKEFTP
                     QDTPIAVKVQALCQVLIDYVSAGHFEIYHKLVKEAELCNHDCQDLISRIMPKIQDSTE
                     LALAFNDRYASVELCEKSMPLLAKDLNKLGMVMVERFDLEDQLIDRLHNRLREQVA"
     gene            complement(7696..8436)
                     /locus_tag="VFX11_09310"
     CDS             complement(7696..8436)
                     /locus_tag="VFX11_09310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX4938860.1"
                     /translation="MKKWVLAALCTALVACNEGENGSTSVIQNAQAEDKAAVSLTTED
                     QKASYAIGLRYGEGFARDLKEIDLAVFYKGLQDGFKGTPPQMKPEEIMATLQALQSRK
                     MEEQQKEQAKAADGNKASSDAFLAKNKERKEVTTTASGLQYEVIKKGSGETPDENDKV
                     NVHYHGTLPDGTVFDSSVDRGEPISFAVNGVIKGWTEALQLMHVGDKWKLFIPADLAY
                     GPRGAGPKIGPNQALVFDVELLGVEKVN"
     gene            8696..9673
                     /locus_tag="VFX11_09315"
     CDS             8696..9673
                     /locus_tag="VFX11_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938861.1"
                     /translation="MAAKKAAVKPKAAKKNPVVELSAQIAKLQDQLNKTIEKAAADAS
                     KNAAAAKGALDKAQAAAKKKSDAKTTAAVAAAKAALSKAQAEEASAKRELASAKKVAQ
                     AVATVKKAAAKADKAEAKKAAAKAKVEAKKAAAAKKAAAKKAKAAKKAAPKKAGKKAA
                     KPVKAKKAPKAAKAAKAPKAKAAKAAKAPKAAKAPKAPKAPKAPKAAKAPKAAKAPKA
                     EAKPAAAKPVAAKVEAPKAAKPAAPKAEAKPAAAKPAAPKPVAAPKPAAAKPAAPKPA
                     APKPAAAKPAPAPAPAAEAPKPEEKPFELKPVEAPTAPGRSVFDPASDA"
     gene            9857..>10128
                     /locus_tag="VFX11_09320"
     CDS             9857..>10128
                     /locus_tag="VFX11_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4938862.1"
                     /translation="MIQLKSVSLFRGSKCLLKDASFTGYPGWHIALIGHNGCGKSSLF
                     AMLRRELQPDSGEFSIPANWRIAHMAQEVEALDRAALDYVMDGDKTL"
BASE COUNT         2244 a         3147 c         2789 g         1948 t
ORIGIN      
        1 agacgccaac ctgctgggca agctgcgggc atttctgttc agcaacggca tcctcaccgc
       61 gaaactggtg gaaggcaaag aggaagccgg cgccaaattc cgcgattatt tcgatcacaa
      121 ggagccgctg aaatccgcac cctcgcaccg cgcgctggcg attttccgcg gtcgcaatga
      181 aggcatcctg caggccagca tcaccatcga gcaggacgac gaaaacgccc gccacccgtg
      241 cgaggacatg attgcacagc agttcgacat ccgcgacaac ggccgccccg ccgacaaatg
      301 gctgcaggaa gtggtgcgct ggacctggaa gatcaagctg ctcacccacc tggagaccga
      361 tctgctgggc gaactgcgcg aaaaagccga gacggaagcg atcaaggtgt ttgccatgaa
      421 cctgaaggat ctgctgatgg ccgcgcccgc cggcccccgc gccaccatgg gcctggaccc
      481 tggcctgcgc accggcgtga aggtagcggt ggtggattcc accggccagc tgctggatca
      541 cggcgccatt tttccccacg aaccgcgcaa ccagtgggat gagtcggtga aagtgctggc
      601 gcatatggtg aagaagtaca acgtggacct gatcgccatc ggcaacggca ccgcatcgcg
      661 cgagaccgac aagctggcag gggaactcat caagaaatac ccggaattga aagtgcagaa
      721 gatcgtggtg agcgaggcgg gtgcgtcggt gtattccgcc tccgaactag cggccaagga
      781 attccccaaa ctggacgtgt cctatcgcgg cgccgtgtcg attgcccgcc gtctgcagga
      841 cccgctggcc gaactggtga agatcgatcc caaatccatt ggcgtgggcc agtaccagca
      901 cgacgtgtcg cagacccaac tcgcgcgcag cctggacgcg gtggtggaag actgcgtaaa
      961 cgccgtgggc gtggacgtga ataccgcatc ggaagccttg ctggcacgga tttccggttt
     1021 gaacaaaacc atcgccggca atatcgtgaa tttccgcaat gccaacggcc agttccccag
     1081 ccgcgacgcg ctgaagcagg tgccgcgcct gggcgacaaa acctttgaac tgtcggcggg
     1141 cttcctgcgc atcatgaacg gcagcaatcc gctggaccgg tccgccgtgc acccggaagc
     1201 ctacccggta gtggaaaaga tcgcgcagaa aaacgagcgc caggtggcgc aggtgattgg
     1261 cgatgttggc ttcctgaaac gcctcgccgc caacgattac gtggacgaca aattcggtct
     1321 gcccactgtc accgacatcc tgaaggaact ggaaaaaccc ggccgcgacc cgcgccctga
     1381 attcaagacg gctgaattca aggaaggcgt cgagacgatg gaggacctga agccgggcat
     1441 gatcctggaa ggcgtggtca ccaacgtcac caacttcggc gcgttcgtgg atatcggcgt
     1501 gcatcaggat ggcctggtgc acatctcgct gctgtcgaac acctacgtgg ccgatccgcg
     1561 cgaggtggtg aaggccggcg acatcgtgca ggtgaaagtg ctggagatcg atctgccgcg
     1621 caaacgcatc agcctgagca tgaagctgga tgaagcagcc ggccagaaga tcgacggcaa
     1681 gcgccccacc accaacaagt tcggcaaagc ggtggagcag aagagcagcg ggcccaaacc
     1741 cggcgccaaa ggccccgcca atgcgcccgg caaatccggc acgtttgcgg atctgtttgc
     1801 caacgcgaag aacctgcgcg acaagaagtg aagttacctg ggcacaaaaa aagggactcg
     1861 atggagtccc tttttcatgc cgaattgaaa gaacgggtga ttgcgcgtca caccagcacg
     1921 atacggtcct gctcgatccg gatttttttc tgcttgaaca ggttgccgat ggctttcttg
     1981 taactggcct tgctgacgcg atactggcga tagatatcgt cgggcgaact cttgtcggtc
     2041 agcgtcgaca cgccgttgtt gtcctgcagg tgttgcagga tcttatcggc cagctcgtca
     2101 cgggtcgcct gggtctgcag gtgcaggcac agatcaatct tgccgtcgtc gcgtattgcc
     2161 ttgacatagc ctttcagctt ctggccgatg cgcaccggct gtagcaagtc cgaattgaac
     2221 aacataccca ggaagcgatt gtcgatcacc gccttgtagc caaggtcggt gcggccgcac
     2281 accagcaggc tgacaggctg ctgcggcttc aggctgctgg ttttttcctt caggtgccgg
     2341 tgcagcttgg acgtggcggc aatgcgcccg gtctcttcat cgcaaaatac gtgcaccacg
     2401 taggatttgc ccacttccat gggtttgtac tgctcgctga agggcaccag cagatccttg
     2461 ggcaggcccc agtccagaaa ggcgcccacc gcattcaccg ccaccacttt cagataggcg
     2521 cattcgccca cctgggcccg gggcttgtcg gtggtggcca ccagacggtc ttcggaatcg
     2581 acatagacaa acacatcaac gtcgtcaccc acggcgcacc cggccggcac gtaacggcgc
     2641 ggcagcagga tgtcgcccag ttcctggcca tccagataca cgccgaaatc caccgcgcgc
     2701 agcacgcgca gacggttgta ctggccaatg cgggtttggt tttcttcagc tttcaatgac
     2761 aggtctccgg acaccctgcg acggtgtcgt gtgatggcag cccgggcacg tccggacttt
     2821 accggcatta tatgcggtca tcctgaccct gtcccccgcg cggaggccca acctcccgcc
     2881 gcgaacgcat aacgttaatc tgactgtcag aaagaattca ttgcccgatc agatatccga
     2941 cacgtttccc cgccacactg gctctgcttt ggggggaagc gacagacagg acgttgcgtt
     3001 ttacgggttc ttgttgtggc cggcttccgg ttgccgctgc caatgcgcgc aggggtgaca
     3061 agcgatttca ggaccaacaa caatccgcaa cctgtctgaa aggagtttct gccatgcagt
     3121 caccgttacg caaatgcctg cccattctgg ccatgacggc ctccaccctg tttgccctgc
     3181 catccgccca ggccgccttc gtgatgaccc tggacgatcc caatgacagc ctgtccgccg
     3241 tcactatcta cgatggcacc ggcggcgatc tgaacggcgt caatggcgtg atcacctaca
     3301 gcggctccgt gggtgcattc atggtgaacg tcaccaccgg cgtcagcaaa cctctcatcg
     3361 gcccggcaca gctggatctg aacagcatca acgtcagcgg cgcggccggc acgctgctgg
     3421 tgtccatcag cgataccggt ttcaccggca atttcaacgc gctgggcgcc agttatggcg
     3481 gcactaccca gggcgccgtc gatttcgatt tcctgtatga cactgccaac cagcaattcg
     3541 gcggcagcgc atttgccacg gacagcttca cctccggcgg cacccactat gcgttctcga
     3601 atgatctgac cggcgccgtc acacctgccg cactcttctc cctcaccatc agcacccgca
     3661 tcacccacga ttctgccttc gaagtcagca gctttgacgc gcagatcgca ccggtgccgc
     3721 tgcccgccac catctggtta ttgggcgcgg ccatggcggg tctggccacg ctgcgccgcg
     3781 cccgtcgcag ctgaacgcgt ttgttcccac gttccggggc ctctgcggcc ccggaacaga
     3841 gcacaccccc gcacacaatc tccgaccgat tttgcgcggc agtatcgacc atttccaacc
     3901 gccgcccctt cccgcataat gcccctcagc ttcgttaccc tgcacaggaa taatgcgtga
     3961 ccaagaccct actgcaccgg cttgaatcca ttcgcgacac acggctgcat cacacgctga
     4021 ccacgctcaa gcgcggcatc gaaaaggaaa gcctgcgggt gacaccgaac ggcaccctgt
     4081 cgcaggcccg gcacccggaa gcactgggct cggcccttac ccatcaatgg atcaccaccg
     4141 attattccga agcgctgctg gaattcatta cccccgcgtt tcaggatctg caccgtccgc
     4201 tgcggtttct caatgatgta caccgcttca cctaccagca tatcggcgag gaaaaaatct
     4261 gggtcaacag catgccgtgt gtgctgggcg acgaactcag cattccgatt gcagaatacg
     4321 gcacctccaa ttccgggcgc atgaagcacg tttaccgcca cggcctttgg caccgttacg
     4381 gccgctacat gcagaccatt gccggcattc attacaacct gtcgattcct gacaatttct
     4441 ggcaggtcta ccaccagctg cagaacagcc agcagtcgct gcaggatttc atttccgacc
     4501 agtatttcgc gctgatccgc aatttccagc gtaacgtggg gctggcggtg tatctgtacg
     4561 gcgcgtcgcc ggcggtgtgc acctcgttcc tgcagggccg caaccacaag ctggaaacgc
     4621 tcaacaagca caccctgttc agccggtacg gcacatccct gcgcatgagc gatctgggtt
     4681 atcacaacga cgcccagagc gggctcgaca tttcctacaa ttcgctgggc gaatacgtgc
     4741 gctcgctgca acacgcgatc cgcacgccct acagtgaata cgaaaagatc ggcgtgctga
     4801 aaaacggcga ataccagcag ctgaacacca atatcttgca gatcgaaaac gagttttaca
     4861 gcagcatccg gcccaagcgc gtgagcaagc gcggcgagcg gcccacctgc gcgctggcat
     4921 cgcgcggcgt ggaatatgtc gaactgcgct gcgtggatct ggacccgttc acacccatgg
     4981 gcatcaacga aggccagatg atgttcctgg acgtattcgc gctgtactgc ctgctggaag
     5041 aaagcccgcc catcgacgaa aaggaaaaaa cctgcaattc gcaaaacctg caggccatcg
     5101 tgatgcacgg ccgcaatccg gagatgaaac tggaatccaa atgcgtgaaa atgccgttcc
     5161 gccagtgggc acagcagcac ctggacaaca tgctgcaggt ggcccgcctg ttcgacatgg
     5221 cctacgacaa cgatctgcac acaaaggtgg tgaaacagca gatggaaaaa ctgcgcgatc
     5281 cgcacctgac accatcgtcg aaggtcatcc gccagctgga cgaacgcgac cagtcatttt
     5341 tccagttcgc catgcattgc gcgctggagc aggaaaaaca tttccgcaac aatccgctca
     5401 gccccgagga aaccgtgcct ttcctgcacg aagccaaacg ctcgctggaa gcacaaagac
     5461 gcatggaagc cagtgacaac gtgtcctttg agcagtatct tgagagctac ttctcaccgg
     5521 atgtcgtgtg ctgaagtgcg ggggccccat ccatcttcac ttaattcctt gaattttttt
     5581 ggcttttgcc aacaagtcat cgttttggcg cacggtgcaa acataaaaaa tcaacgagtt
     5641 accgtgactt cctagaatcg actgctaatc ttgatttcag gtaccgcatt tgcggcatct
     5701 tttatggcag tcaattccag gtcaccgcta ttcagaggca aggagccgat gacttttaaa
     5761 gaaatcgaca accagaactg gagtatcgag accctgaaca aggcttaccg tcaggggtac
     5821 atgtttggct tgagtgggga aaatcagggt tcctgcccct acaagtccga cgtcattgcc
     5881 gcagcctggg aagcaggctg gctcgacggc cgcacgcagg cggagttcgt cagcagcatg
     5941 gaatccgact gcgccatcgc gtaactcttt cgcgatacag acggaatttt ttccggcggg
     6001 aacgggtcta tcgtcacagt gcactggccg attgtgttta taatcggcgc gccatccatc
     6061 acgggttctg acgactgact atgaatttac cctcatcccg cctctgcaat gccgcgggcg
     6121 tggcaggctc catcgtcgcc atgctgttcg cgtactacta tctccagcgc acgctggggc
     6181 tggatccctg tcccctctgt ctgatcgatc gcggcctggt catgctggcc ggcttcttct
     6241 ttctgctcgc tttcattcac aatcccggcc gcaccggcca gcgggtttac agcgggcttg
     6301 cactgattcc ggccctgctg ggtgttgcgg tgtgctggcg tcacctgtgg ctggaaagcc
     6361 tgcccaagca tctggtgcca gaatgcagcc cgggactgga gtacatgctg gaaacgtttc
     6421 ccatcggcga aaccatccgc accatattca acagcgccgg cgagtgcgcc gaggtgaagt
     6481 ggcgcttcct gggcttgtcc atcgccgggc agaccctgct ggtttttctg ggttttactg
     6541 cgctctccct gctgcagatc ctgcgcaagc gcgactgaat cgtcgcgctt caaccccact
     6601 cttttagact ttgttcttct aagaatttcg cggccaatag cccgcatccg cactaaatcc
     6661 ctttttcgcc tccccagtca aagcccacct gtttgtcaac gtgacatgca acaactttgt
     6721 gccgaaagcg tcgcttgctg ccgcggatga atttacatat tctcgaaagt ccgcagtcat
     6781 tcaaaagcag aatggcgcgg tgattcaatc atctccggtg attggatata cacagacaat
     6841 ttgagaaaga cctgcattct tgcaacccaa attggagcgg tccttaccgc atccgttgtg
     6901 taaaacggta acagtgattc atcacactta cccgtccgag caacaagacc agaaggaatt
     6961 accacatgct gaccgaagcc acttctccca ctgatcattg gggttctgta gacaagctga
     7021 tcaagcactg gttgaaggaa cgccaggaac tcatcgtcct ctattgcaag gtggacggac
     7081 tcaaggaatt cacgccgcag gacaccccca ttgccgtcaa ggtccaggcc ctgtgccagg
     7141 tgctgatcga ctatgtgtcg gcaggccatt tcgagattta tcacaagctg gtgaaggaag
     7201 cggaactgtg caatcacgat tgccaggatc tgatttcccg catcatgccc aagatccagg
     7261 attccaccga actggcgctg gcgttcaacg accgctatgc ctcggtggag ctatgcgaga
     7321 aatccatgcc gctgctggcc aaagacctga acaagctggg catggtgatg gtggaacgct
     7381 tcgacctgga agaccagctg atcgaccgcc tgcacaaccg tctgcgcgaa caggtcgcct
     7441 gaccgtttgc atcccgccgg gttgacgctg tcggcccggc cattttctcc ctcctgcttt
     7501 cacccgttaa ccgtacatcc ttcaggcgtt atgcatgcca gtggcaccgg ccggtgtgac
     7561 gggcgcgcgt gcgcgatgga aggctctacg tgcagcgttt ctgagtgaag gagcgaggga
     7621 ataagggggc gtgcgggaaa ggaggatgga aagccatcga agcggcgctg aacgccgctt
     7681 cgacagggga caggatcagt tgaccttttc cacgcccagc agttctacat caaataccag
     7741 cgcctggttg gggccgatct tgggaccggc gccacgggga ccgtaagcca ggtcggcagg
     7801 aatgaacagc ttccacttgt cgcccacatg catcagctgc agggcttcag tccagccctt
     7861 gatgacgccg ttcacggcaa aggaaatggg ttcgccccga tccaccgagc tgtcgaacac
     7921 ggtgccgtcc ggcagggtgc cgtgatagtg cacgttcacc ttgtcgttct cgtccggggt
     7981 ttcgccgctg cctttcttga tcacttcata ctgcagaccg ctggcagtgg tggtgacttc
     8041 cttgcgttcc ttgttcttgg ccaggaaggc atcgctggag gccttgttgc catcggcagc
     8101 cttggcctgt tctttctgct gctcttccat cttgcgggac tgcagcgcct gcagggtagc
     8161 catgatctct tccggcttca tttgcggcgg agtgcctttg aaaccatcct gcagcccctt
     8221 gtagaacacc gccagatcga tttccttcag atcgcgcgca aagccttcgc cataacgcag
     8281 accgatggca tagctggcct tctggtcttc ggtggtcaga ctcactgcag ccttgtcttc
     8341 cgcctgggca ttctgaatca cgctggtgct cccgttttcg ccttcgttac aggcaaccag
     8401 tgcagtgcac agtgctgcca aaacccactt cttcataatt cctcccgttc tccgccatcc
     8461 tgctcatcga cagcctgatg gatgcaaaaa gcttgtcccg atgcgattgc ggccgttgtt
     8521 gtcgttaatt gcaaccgggg ccgatactag cagaatcccc tgcatgcgga accgtttcgc
     8581 ccgttttttt tccgactgaa tgtggccggg ctatcacaag aaagtagata ttgtccaaac
     8641 aactcgtata attgctagcg actgtgccac ctaaatcagc tgaggagcga tgactatggc
     8701 agccaaaaaa gccgcggtga aacccaaagc cgccaagaaa aatcctgtgg tcgagctctc
     8761 ggcccagatc gcaaaactgc aagaccaact gaacaaaacc atcgaaaaag ccgcagcaga
     8821 tgcttccaag aatgcggctg ccgccaaagg cgctctggac aaagcccagg ccgctgccaa
     8881 gaaaaaatcc gatgccaaga ccacagccgc tgttgctgcc gccaaagctg ccctgagcaa
     8941 ggcgcaagcc gaggaagcaa gtgccaagcg cgaactggcc agcgccaaga aagtggccca
     9001 ggccgttgcg accgtgaaga aagccgccgc caaggccgat aaggcagaag cgaagaaagc
     9061 agccgcaaaa gccaaggtcg aagccaagaa agcggccgct gcgaaaaaag ctgctgccaa
     9121 gaaagccaag gccgccaaga aagctgcacc gaaaaaggct ggcaaaaaag ccgccaaacc
     9181 ggtgaaagcc aaaaaggcac cgaaagccgc caaggctgcg aaggcaccca aggccaaggc
     9241 cgcgaaagct gcaaaagcgc ctaaagccgc gaaagcaccg aaagcaccaa aagcacccaa
     9301 ggctccaaaa gcagcgaaag cacccaaagc tgcaaaggca ccgaaggctg aagccaaacc
     9361 ggcagcagcc aagcctgtag cggcgaaagt ggaagctccc aaggctgcca aaccggctgc
     9421 gcccaaggcc gaagccaaac cggccgctgc aaaaccggcg gctcccaaac ccgttgccgc
     9481 accgaagcct gcagccgcca agcccgccgc tcccaaaccg gcagctccca agcctgcagc
     9541 agcaaaaccg gctcctgcac cggctccggc agcagaagcg cccaagcctg aagaaaagcc
     9601 gtttgaactg aagccggtgg aagcaccgac tgcaccgggc cgcagtgtgt tcgatcccgc
     9661 cagcgacgcg taagcgtttc ccggctgtac aagaagggct gctgcctcag ggcacagccc
     9721 ttttttgtgg cctgccgtcc ctggcggcac cctgcgggcc gtcgctggcg cgacgtaaaa
     9781 aattgctccc ggcaattttt tcatgtccac tatttcgcta ttctgccagc cccgacagcc
     9841 gacacgtcca cgccccatga tccagctcaa atccgtttcg ctttttcgcg gcagcaaatg
     9901 cctgctcaag gatgccagtt tcaccggcta tcccggctgg cacatcgcat tgattggcca
     9961 caatggctgc ggcaagtcct ctctgttcgc gatgctgcgg cgcgaactgc agcccgacag
    10021 cggtgagttt tccattcccg ccaactggcg cattgcccac atggcgcagg aagtggaagc
    10081 cctcgatcgg gccgccctgg actacgtgat ggacggtgac aaaaccct
//
LOCUS       DASUEG010000498         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1404779, whole genome shotgun sequence.
ACCESSION   DASUEG010000498 DASUEG010000000
VERSION     DASUEG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404779"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="VFX11_09325"
     CDS             <1..2200
                     /locus_tag="VFX11_09325"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX4938863.1"
                     /translation="KASENRQAPFLIYQESNVIIRAIRDYLRQDIGEVLVDTQEVFDE
                     AVNFVRQVMPQYESKIKLYKDNIPLFNRYQIESQIETAFEREVKLPSGGSIVIDPTEA
                     LVSIDINSSKATKGADIEETAFNTNLEAAEEIARQLRLRDIGGLIVVDFIDMTNNRNQ
                     REVENRMRESLELDRARVQIGRISRFGLMELSRQRLRPSLGETSGHICPRCNGVGFIR
                     DLHSLSLNIMRLIEEEALKENSSEIHAKVPVNVATYLLNEKRQVLVNIETRNDVRVVI
                     LPSPNLETPHFEVNRFRTDQLPGGEGVASYEMIDEADEVDEVVLTPGETPIKKPEQPA
                     VQMIQPQTPAPQPQEMPAPVPVAVPKEPSFFARLFTWFLQLFSSQQPQPEAKAEEIPN
                     QRDNQRNQRNQQRDRGNRNQRDRGNRNQRDRGQRQERKREDDIVTNLPANKEQRPPQP
                     QQQERKGGQRQERGERQERGERQERGERQEKGERQERGERQDRGDRQRGDRQERGERD
                     QQRNRQPQAESTEETSGGDQGNQRRGKRRSPNDNRRRRQSAAQAEENAQRGQQDGVVG
                     ETGSTEPFEVQAEATAAVATAIAAPESAVAVSEQGSTENRPPRRHEQRPRSESAADDT
                     RRRPRVPQPAEAEASPVPAQPAAEAEPAPEARPPRIEPVAEQQAAPAAPMAITAAVVE
                     EKPAAAVQAADDGSGQRAANDPREIRRRQLAEAAARASESSTAAEAGKDA"
     gene            complement(2230..>2735)
                     /locus_tag="VFX11_09330"
     CDS             complement(2230..>2735)
                     /locus_tag="VFX11_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX4938864.1"
                     /translation="CLITHVVFRLHKRPALKLDYGEIRAEIASQRLSPESLTPLQLRQ
                     IIIAVRARKLPDPRQLPNVGSFFKNPVIPAAQLQTLLASWPNLVHYPLDGAQTKVAAG
                     WLIDQLGWKGRVHGKAKVHERQALVLVNMGGDSEDVLGLARQIQADVRERFRIELEME
                     PLIIGLA"
BASE COUNT          611 a          883 c          826 g          415 t
ORIGIN      
        1 caaggcctcg gaaaaccgcc aggcgccttt cctgatttac caggaaagca atgtgatcat
       61 ccgcgccatt cgcgactacc tgcgccagga tatcggcgaa gtgctggtag atacccagga
      121 agtgttcgac gaagccgtca acttcgtgcg ccaggtgatg ccgcagtacg aaagcaagat
      181 caagctgtac aaggacaaca tccccctctt caaccgctat cagatcgaat cccagatcga
      241 gacggcgttt gaacgggaag tgaaactgcc ttccggcgga tccatcgtaa tcgacccgac
      301 cgaggcgctg gtgtccatcg acatcaactc gtccaaggcc accaagggtg cggacatcga
      361 ggaaacggcg ttcaacacca acctggaagc ggcagaagaa atcgcccgcc agctgcgtct
      421 gcgcgacatt ggcggcctga tcgtggtcga tttcatcgac atgaccaaca accgcaacca
      481 gcgcgaagtt gaaaaccgca tgcgcgagtc gctggaactg gaccgcgccc gggtgcagat
      541 cggccgtatt tcccgtttcg gcctgatgga gctgtcccgc cagcgcctgc ggccgtcgct
      601 gggtgaaacc agtggccata tctgcccgcg ctgcaacgga gttggcttta tccgtgacct
      661 gcactcgctg tcgctgaaca tcatgcgcct gatcgaggaa gaggcactga aggaaaacag
      721 ttccgaaatc cacgccaagg tgccggtgaa cgtggctacc tacctgctga acgaaaagcg
      781 gcaggtgctg gtgaacatcg aaacccgtaa cgatgtgcgc gtggtgattc tgcccagccc
      841 gaacctggaa accccgcact tcgaggtgaa ccggttccgg actgatcagt tgccgggcgg
      901 cgaaggcgtt gccagctatg aaatgatcga cgaggcggat gaagtcgacg aggtggtgct
      961 gacccccggc gaaaccccga tcaagaagcc ggaacagccc gcggtgcaaa tgatccagcc
     1021 ccagactccg gcaccccagc cccaggaaat gccggcgcca gtgcccgtcg ccgtaccgaa
     1081 ggagcccagc ttcttcgccc gcctgtttac ctggttcctg caactgttca gcagccagca
     1141 gccgcagccg gaagccaagg ctgaagagat ccccaatcag cgcgacaacc agcgcaatca
     1201 gcgtaaccag cagcgcgatc gtggcaaccg caaccagcgt gaccgcggca accgcaacca
     1261 gcgcgaccgg ggccagcgcc aggaacgcaa acgcgaagac gatatcgtta ccaacctgcc
     1321 tgccaacaag gagcagcgtc caccccagcc acagcagcag gagcgcaagg gtggccagcg
     1381 ccaggaacgc ggtgaaagac aggagcgtgg tgagcgtcag gaacgcggtg agcgccagga
     1441 aaaaggtgaa cgtcaggaac gcggcgaacg tcaggatcgt ggtgatcgcc agcgtggtga
     1501 ccggcaggag cgtggtgagc gcgaccagca gcgcaaccgc caaccccagg ctgaaagcac
     1561 tgaggagacc agcggtggcg atcagggtaa ccagcgccgc ggcaagcgtc gcagccccaa
     1621 cgacaatcgc cgtcgtcgcc agagcgctgc ccaggccgag gaaaacgccc agcgcggcca
     1681 gcaggacggt gtggtagggg aaaccggcag cactgagcca ttcgaggtgc aggcagaagc
     1741 aactgctgca gtagccactg caatcgcagc acctgagagt gccgttgcag ttagcgagca
     1801 aggttcgaca gaaaaccgtc cgccgcgccg tcacgagcag cgtcctcgtt ccgaatcggc
     1861 tgctgacgac actcgccgcc ggccccgggt accacaacct gctgaagctg aagccagccc
     1921 tgtaccggct caaccggctg cagaagcaga acctgccccg gaagcccgtc cgccgcgcat
     1981 tgagccggtg gccgagcaac aggctgctcc tgccgctccg atggcgatca ccgccgccgt
     2041 agtggaggaa aagccggctg ctgccgttca ggccgcagac gatggcagtg gccagcgcgc
     2101 tgcaaacgat ccgcgtgaaa tccgtcgccg ccagctggcg gaagcggctg cccgcgcatc
     2161 ggagtcatcc acggctgccg aagccggcaa ggacgcctga tccaccctga aaaaaatcgg
     2221 gctggtgttt tacgccagcc cgatgatcag gggttccatt tccagctcaa tcctgaatcg
     2281 ttcccgcaca tctgcctgta tctggcgggc caatcccagc acgtcctcac tgtcaccccc
     2341 catattcacc agcaccagcg cctgccgttc gtggaccttt gccttgccgt gcacccgccc
     2401 cttccagccc aactggtcga tcagccagcc cgccgccact tttgtctgcg ccccatccag
     2461 cgggtaatgc accaggttcg gccaactggc cagcagggtc tgcaactgcg cggcaggaat
     2521 aacgggattc ttgaaaaaac tgcccacgtt gggaagctgg cgcgggtccg gcagtttgcg
     2581 cgcacgcacc gcgatgatga tctggcgcag ttgcagtggc gtgagggatt cggggctcaa
     2641 cctctggctg gcaatttcgg cgcggatttc gccgtaatcc agcttcagcg ccggacgttt
     2701 gtgcagacgg aacaccacat gggtgatcag gcagc
//
LOCUS       DASUEG010000499         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1492154, whole genome shotgun sequence.
ACCESSION   DASUEG010000499 DASUEG010000000
VERSION     DASUEG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492154"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFX11_09335"
     CDS             complement(<1..657)
                     /locus_tag="VFX11_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4938865.1"
                     /translation="MQGHESVWIIDDDKSIRWVLEKALNQEGINTSSFETGDSALNKL
                     RNTQPDVIITDIRMPGTDGLGLLAHIRDHHPDLPVIIMTAHSDLDSAVQSYQGGAFEY
                     LPKPFDLDDAVSLVKRAVTHSQEQQSSTIEPPLTSSTDIIGEAPAMQEVFRAIGRLAL
                     SHATVLITGESGSGKELVARALHRHSPRADKPFIAINTAAIPRDLLESELFGHERGSF
                     T"
     gene            complement(662..1759)
                     /gene="glnL"
                     /locus_tag="VFX11_09340"
     CDS             complement(662..1759)
                     /gene="glnL"
                     /locus_tag="VFX11_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX4938866.1"
                     /translation="MNYDLPKSLLDNLTTAVLLLDESLRVRYLNPAAEHLLAASLSQL
                     VGTPIRQLISENDPNAHADMETALAELHPYTKREAQLHVANTPHAATVDYTITPLVEP
                     GYPRSLLMEIRPLDRLMRISREDGIINAHQATRALVRGVAHEVKNPLGGIRGAAQLLA
                     RELPDETLREYTDIIIDEVDRLRNLVDRMLGPRKLPSLAQVNIHEILERVRAVLLAEG
                     DEIAVERDYDPSLPELWGDKDQLIQAILNIVRNARQALRENPGQKDPKIIIRTRALRQ
                     FTIGHTRHRLILLVEVEDNGPGVDKELQETMFYPMVSGRASGTGLGLSISQSIVNQHH
                     GLIECDSQPGKTIFRLLLPLEKPEGTSHIGV"
     gene            complement(2085..2645)
                     /locus_tag="VFX11_09345"
     CDS             complement(2085..2645)
                     /locus_tag="VFX11_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4938867.1"
                     /translation="MARTLLALLVLLTAWRPALAETVYKYVDENGNTVFTDEPRKGAE
                     QMDVKPIPTVPALPTQAPQKQPAAAGPFQYNKILIVSPEDQHNFINETDPVVVQAAVS
                     PNLRTADRVQLMLNGSPHGSPISSTAFTLDSLDRGEYKAQVRILDKDGKELGISDTVT
                     FQVKRNSVNLPGQKKPQPKPKPKPQT"
     gene            complement(2891..>3272)
                     /gene="glnA"
                     /locus_tag="VFX11_09350"
     CDS             complement(2891..>3272)
                     /gene="glnA"
                     /locus_tag="VFX11_09350"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940720.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX4938868.1"
                     /translation="IRIPWVTSAKARRIEARFPDPICNPYLGFAALLMAGLDGIKNKI
                     HPGDAADKDLYDLEPEEAKKIPTVCSSLEQALDTLEQRGDFLLEGGVFSEDMIKAYVE
                     LKRGEVERLNMTTHPIEFDMYYSC"
BASE COUNT          578 a          943 c         1049 g          702 t
ORIGIN      
        1 ggtgaaggaa ccgcgctcat gaccgaacag ttcggattcc agcaggtcgc gcgggatcgc
       61 cgcggtgttg atggcgatga aaggtttgtc ggcgcgcgga ctgtgccggt gcaacgccct
      121 cgccaccagc tccttgcccg aacccgactc gccggtgatc aagacggtcg catgggacag
      181 ggcgagccgg ccgatggcgc gaaacacttc ctgcatcgcc ggcgcttcac cgatgatgtc
      241 ggtgctgctg gtcagcggcg gctcgatggt gctcgactgc tgctcctggc tgtgggtcac
      301 cgcccgcttc accagcgaca cggcgtcatc cagatcgaac ggcttgggca ggtattcgaa
      361 cgcgccgccc tggtaggact gcacggcgct gtccaggtcc gagtgcgccg tcatgatgat
      421 cactggcaga tccgggtgat gatcgcggat gtgcgccagc aggcccaggc catcggtgcc
      481 cggcatgcgg atgtcggtga tgatcacgtc cggctgcgtg ttgcgcagct tgttcagcgc
      541 cgaatcaccg gtctcgaagg acgaggtgtt gatgccttcc tggttgagag ccttttccag
      601 aacccagcga atggacttgt cgtcgtcgat gatccagaca gattcgtgcc cttgcatgcg
      661 cttatactcc aatgtgtgac gtgccttctg gtttctccag cggcagcagt agtctgaaaa
      721 tggttttgcc cggctgactg tcgcactcga tcaggccgtg atgctgattg acgattgact
      781 gtgagatgga aaggcccagg ccagtgccgc tggcgcggcc gctgaccatt ggatagaaca
      841 tggtttcctg cagctccttg tccacgccgg ggccgttgtc ctccacttcc accagcagga
      901 tcaggcgatg ccgcgtatgg ccaatggtga actggcgcag cgcgcgggtg cggatgatga
      961 tcttgggatc tttctggccc gggttctccc gcagtgcctg gcgggcattg cggacgatgt
     1021 tcaggatcgc ctggatcagc tgatccttgt cgccccagag ttccggcagg ctggggtcgt
     1081 aatcccgctc cacggcaatt tcgtcgcctt ctgccagcaa aacagctcgc acccgttcca
     1141 gaatctcgtg gatgttcacc tgcgccagcg agggcagctt gcgcggtccc agcattcggt
     1201 ccaccagatt gcgcaggcga tccacctcat caatgatgat gtcggtgtat tcccgcagcg
     1261 tctcgtcggg cagttcccgc gccagcagct gggctgcgcc gcgaatgccg cccagaggat
     1321 tcttcacttc gtgcgccacg ccccgcacca gcgcccgggt ggcctgatgg gcgttgatga
     1381 tgccgtcctc ccggctgatc cgcatcagac ggtccagtgg gcgaatctcc atcagcaggc
     1441 tgcgcgggta accgggctcc accagcggcg tgatggtgta gtccacggtg gcggcatgcg
     1501 gtgtattggc cacatgcaac tgggcttcgc gcttggtgta gggatgcaac tccgccagcg
     1561 ccgtttccat gtcggcgtgg gcattgggat cgttttcgct gatcagctga cggatcggcg
     1621 ttcccaccag ctgcgacagg ctggcggcca gaagatgctc ggccgcggga ttcaggtagc
     1681 gcacccgcag gctctcatcc agcagcagca cggctgtggt gagattgtcg agcaggcttt
     1741 tcggcaggtc gtaattcatt gaatccggta ctgaatcggg tgaggtgtaa cagtgccgtc
     1801 atggcagctg caacagggtt taattacgag aatgcaaaaa ccaaaccagc atgtccggtt
     1861 cgattcccgc agtcgccatc gggcaggctg ataaccctgg cgatggcagg gtccaaccgg
     1921 tagaaaaagc ggcagtatcg gcgctttggc gcctatgcgc gtcgagttgg ggcaggcctt
     1981 ggccggctgc cccttttcgg gtatccggcc aggctgatga tggcgatttt agttcaggag
     2041 atgaaaagag cgcaccaaaa aagtgcgcaa cagagaggcg gctgtcaggt ctggggtttc
     2101 ggcttgggtt tgggctgcgg ctttttctgg cccggcaggt tcaccgaatt acgcttcacc
     2161 tggaaggtta ccgtatcgct gatgcccagt tccttgccgt ccttgtccag aatgcgtacc
     2221 tgggccttgt attcgccccg gtccagactg tccagggtga aggccgtgga cgagatgggg
     2281 ctgccatggg gactgccgtt gagcatcagc tgcacacgat ccgccgtgcg caggttcggt
     2341 gaaaccgctg cctgcaccac taccggatcg gtttcattga tgaagttgtg ctggtcttcc
     2401 gggctgacaa tcaggatctt gttgtactgg aaagggccgg cggccgccgg ttgcttctgg
     2461 ggtgcctggg tgggcagtgc cggtacggtg ggaatgggtt tcacatccat ctgttcggca
     2521 cccttgcgcg gctcgtcggt aaagacggta ttgccattct cgtccacgta tttgtagacg
     2581 gtttcggcca gggctggacg ccaagcggtc agcagtacaa gcagggccaa taaggtgcgt
     2641 gccatggtgc tctcccaaac atttttgtag tggctgcctt catttaaaac cattttcggc
     2701 cgcccataca aaaaaatcgt ctggaacgat ttttgacgtc gcgccagcga tggcccgcag
     2761 ggtggccgcc agggatagcg gccacaaaaa aagctcccga aagtgggagc ttttttgcag
     2821 attgcccaag cctcccgggt gggcccgggg gctggcggtg ccggaaatcc ggaccgccgg
     2881 catctgatcc ttagcaggag tagtacatgt cgaactcgat cgggtgagtg gtcatgttca
     2941 gacgctccac ttcgccgcgc ttcagttcca cgtaggcctt gatcatgtcc tcggagaaca
     3001 cgccgccttc cagcaggaag tcgccgcgct gttccagggt atccagtgcc tgttccagcg
     3061 aggagcagac agtggggatc ttcttggctt cttccggctc cagatcgtac agatccttgt
     3121 cggcagcatc gccagggtgg atcttgttct tgataccgtc cagaccggcc atcagcaggg
     3181 ccgcgaagcc caggtagggg ttgcagatcg ggtcagggaa gcgcgcttcg atacggcggg
     3241 ccttggcaga agtaacccac ggaatccgga tg
//
LOCUS       DASUEG010000500         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_856695, whole genome shotgun sequence.
ACCESSION   DASUEG010000500 DASUEG010000000
VERSION     DASUEG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856695"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFX11_09355"
     CDS             complement(<1..184)
                     /locus_tag="VFX11_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938869.1"
                     /translation="MNPSADARDDQTALPQRRVRSFVRRQGRLTEGQNRALETLWDDY
                     GLTLDAGMLDFAALFGN"
     gene            complement(250..1032)
                     /locus_tag="VFX11_09360"
     CDS             complement(250..1032)
                     /locus_tag="VFX11_09360"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX4938870.1"
                     /translation="MTDQPLIVGTRSFQSRLLVGTGKYKDMAETAAAIEASGAEIVTV
                     AVRRTNIGQNPGEPNLLDVISPDRYTILPNTAGCYNATDAVRTCRLARELLDGHNLVK
                     LEVLGDEKTLYPNVVETLKATEILIKDGFEIMVYTSDDPIIAKELEQMGCVSVMPLAA
                     PIGSGLGIRNPYNIRMILEEAKVPIIVDAGVGTASDAAIAMELGCHGVLMNTAIAKAQ
                     NPVLMASAMKKAIEAGREAFLAGRMPKKLYASASSPIEGTFF"
     gene            complement(1079..1297)
                     /gene="thiS"
                     /locus_tag="VFX11_09365"
     CDS             complement(1079..1297)
                     /gene="thiS"
                     /locus_tag="VFX11_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX4938871.1"
                     /translation="MNPASTLSVSVNGETRQWPAGSTIADVVAALQLTGKRIAVERNQ
                     EIVPRAQHGATQLQAGDQVEIVQAIGGG"
     gene            complement(1374..1742)
                     /locus_tag="VFX11_09370"
     CDS             complement(1374..1742)
                     /locus_tag="VFX11_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX4938872.1"
                     /translation="MKTFIVIGAVAMALGVVLGAFGAHGLKARLTPDLLAVYQTGVEY
                     HLYHALGLILVGILVVQFPHISGLKVGGWLLLAGILVFSGSLYVLAISGVRWLGAITP
                     IGGLAFIVGWSWIAWSLARG"
     gene            1952..3133
                     /locus_tag="VFX11_09375"
     CDS             1952..3133
                     /locus_tag="VFX11_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX4938873.1"
                     /translation="MFHYLKYFLFHGIGLLSALFFTLGGDWITGGFIGIFVGYVALDA
                     LLGDDISIPDFRFPGVLTVQLWLALPLLSLICFIALWQVSDGDPLGYGALVQSLTGYD
                     MFAAREANTGWNLFWGVIMCGLMIGMVGTITGHELTHRTWDKVSMFVGRWLLAFSFDS
                     NFSIEHVYGHHRYVSTTEDPATAPRGRNVYAHILISTLRGNASAWKIERDRLAKKDQG
                     LFSWHNVALRGFLMSVVLLGLAYLIGEWTAVAYFIACGLWGKALLEIVNYMEHYGMVR
                     NPSTPVQPRHSWNTNRRLSSWTMFNLTRHSHHHAQGEVPYHALKPYHEAPMMINGYLA
                     TIAVALIPPLWHKLMTPKVLDWDARYATAEELKLAQAANQKSGLKSLQDVRYENNLQT
                     V"
     gene            complement(3224..>4125)
                     /locus_tag="VFX11_09380"
     CDS             complement(3224..>4125)
                     /locus_tag="VFX11_09380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4938874.1"
                     /translation="FAIRGTFVATFMNKYSGLPMALTGALSSETSVQRVKETASFFTT
                     ATLPGALGRYGAGFKAAALVRLMHSMVRFNILKRSKKWDVSVYGIPIPQVDQMPAGTI
                     PAFTMAFNVVQRGRKHFNKRERAVVELCRYQSYLLGLPEDLLPDTPQGIVDTMLTYAG
                     TLRDGYDDETCGELVRSTMSAYLPPDKSLKNQIYNQFEKSFSKVFFGRVFLAGYEKSR
                     ADMMGVKPTPLDYALFVATSAYVMPQMLANRVIQDIPIANRILDKILIRRIDDLLADY
                     GHAEYTTDPSRYKEAPVAQPQSA"
BASE COUNT          831 a         1270 c         1172 g          852 t
ORIGIN      
        1 cgttgccgaa cagtgcggca aaatccagca tgccggcatc cagtgtcagg ccgtaatcgt
       61 cccacagggt ttccagcgcg cggttctggc cttcggtcag gcggccctga cggcgcacaa
      121 aactgcgcac acggcgctgc ggaagggctg tttgatcatc gcgcgcatcc gcagacggat
      181 tcagttcggg attcacaccg tcaccggcaa cagaggactc gtcaacgcca gagtcagaaa
      241 tgaaagcact cagaaaaaag ttccttcaat gggcgacgac gcactggcgt agagtttttt
      301 cggcatgcgt ccggccagga aggcttcgcg cccggcctcg atcgcttttt tcatggccga
      361 tgccatcagc accggattct gcgccttggc aatcgccgtg ttcatcagca caccgtggca
      421 gcccagttcc atcgcaatcg ccgcgtcgga cgcggtgccc acgccggcat cgacgatgat
      481 cggcactttc gcctcttcca gaatcatgcg gatattgtag ggattgcgaa tgcccagacc
      541 gctgccaatc ggcgccgcca gcggcatcac tgacacgcag cccatctgct ccagttcctt
      601 ggcaataatc ggatcgtccg aggtgtacac catgatctcg aaaccgtcct tgatcagaat
      661 ctcggtggct ttcagggttt ccaccacatt ggggtacagg gttttctcgt cgcccagcac
      721 ttccagtttc accaggttgt ggccatcgag caattcgcgg gccaggcggc aggtgcgcac
      781 cgcgtcggta gcgttatagc agccggcggt gttgggcagg atggtgtagc ggtcggggga
      841 aatcacgtcc agcagattgg gttcgccagg attctggccg atgttggtgc gacgcacggc
      901 gacggtgacg atctcggcgc cactggcttc gatggcagca gcggtttcgg ccatgtcctt
      961 gtacttgcca gtgcccacca gcaggcgcga ctgaaacgag cgggttccga cgatgagggg
     1021 ttggtctgtc atgctgcctc tgttgcgggt gcgaatgttg taagtgcgaa cggcgctgtc
     1081 aaccgccgcc gatggcctgg acgatttcca cctggtcacc cgcctgtaat tgcgtggcgc
     1141 catgctgggc ccggggcaca atttcctggt tgcgctccac cgcgatgcgc ttgccggtca
     1201 actgcagcgc ggccaccacg tcggcgatgg tactgccggc cggccactgc cgggtttcgc
     1261 cgttgacgct gacagaaagg gttgaggccg gattcatgga ctgctctcgc ggatcggatg
     1321 ctgggatcag ggtcgtctat tgtcgggaaa cgggggcggc cagtaacggg cattcaaccc
     1381 cgggccaggc tccaggcgat ccacgaccaa cccacgataa atgccagtcc gccaatgggt
     1441 gtaatcgccc ccagccagcg cacaccgctg atggccagca catacagact gccggagaac
     1501 accagaatcc ccgccagcag cagccacccg cccaccttca atccacttat atgaggaaac
     1561 tggaccacca gaatccccac cagaatcagt cccagcgcgt ggtacaggtg gtattccacc
     1621 ccggtctggt acaccgccag caggtccggc gtcagccggg ccttcagtcc gtgggcaccg
     1681 aacgccccca gcaccacccc cagcgccatg gctaccgcac ctatcacaat gaaagtcttc
     1741 accttttatt ccctcttccc gcacacgccg atggcgcagt gtaaagaccg gcccggttcc
     1801 ggctcaacca ccgttacgca aaccacacaa atttcccgcc ccgccggggc ttgtctcccg
     1861 ttcggggtat cggtatattt tgtccgattg taggacaata ttgattttca taattccccg
     1921 acaagccagt agacaaaaaa gaggcagccc catgttccac tatctgaaat attttctctt
     1981 ccacggcatc ggtttgctgt cggccctgtt tttcaccctg ggcggcgact ggatcaccgg
     2041 gggcttcatc ggcatcttcg tcggctacgt ggcgctggat gccctgctgg gtgacgacat
     2101 ttccattccg gatttcagat ttcccggtgt gctcactgtg caactctggc tggcactgcc
     2161 gctgctgtcc ctgatctgct tcatcgccct ctggcaggtg agcgacggcg atccgctggg
     2221 ctacggcgcg ctggtgcaat ccctcaccgg ttatgacatg ttcgcggcgc gggaagccaa
     2281 cactggctgg aacctgttct ggggcgtgat catgtgcggc ctgatgatcg gcatggtggg
     2341 caccatcacc ggccacgaac tcacccaccg cacctgggac aaggtgtcga tgttcgtcgg
     2401 ccgctggctg ctggccttca gcttcgacag caatttttcc atcgagcacg tgtacggcca
     2461 ccaccgctac gtatccacca ccgaagatcc cgccaccgcc ccgcgtggcc gcaatgtcta
     2521 tgcgcacatc ctgatttcca ccctgcgcgg caatgccagc gcgtggaaaa tcgaacgcga
     2581 tcggttagca aaaaaggatc agggcctgtt ttcctggcac aacgtggcac tgcgcggttt
     2641 tctgatgagc gtcgtgttgc tggggctggc gtatctgatc ggtgagtgga ccgccgtggc
     2701 gtatttcatt gcctgcggtc tgtggggcaa ggcgctgctg gaaatcgtga attacatgga
     2761 acactacggc atggtgcgca acccttccac accggtgcag ccacgtcatt cctggaacac
     2821 caaccggcgc ctgagttcct ggaccatgtt caaccttacc cgccactcgc atcatcacgc
     2881 gcagggcgaa gtgccctacc acgcactgaa gccgtatcac gaggcgccga tgatgatcaa
     2941 cggttacctg gcgacgattg ccgtggcact gattccgcca ctgtggcaca agctgatgac
     3001 accgaaagtg ctggactggg atgcgcgtta cgccactgcg gaggaattga aactggcgca
     3061 ggcagccaac cagaagagtg gattgaaatc actgcaggac gtgcgttacg aaaacaactt
     3121 acaaactgtt tgactgaaga ttgccaatct acgggaacca tcccgttttg caggccggca
     3181 gtttactgcc ggcctttttt gttgcgctga tcggcaaggc acgtcaggca gactgcggtt
     3241 gcgccaccgg cgcctccttg tagcgggatg gatcggtggt gtattccgcg tgcccataat
     3301 ctgccagcag atcatcgatg cggcgaatca ggatcttgtc caggatgcgg ttggcgatgg
     3361 gaatatcctg aatgacccgg ttcgccagca tctgcggcat tacataggca ctggtggcaa
     3421 caaagagcgc ataatccagc ggcgtcggtt tcacgcccat catgtccgcg cgcgattttt
     3481 catacccggc caggaacaca cgaccaaaaa acaccttgga gaaactcttt tcaaactggt
     3541 tgtagatctg gtttttcagc gacttgtccg gtggcagata cgcgctcatg gtggaacgca
     3601 ccagctcgcc acaggtttca tcgtcatagc catcgcgcaa cgtgccggca taggtcagca
     3661 tcgtgtcgac aattccctgt ggcgtatctg gcagcaaatc ctcgggcagc cccagcagat
     3721 aactctgata gcggcacagt tccaccaccg cgcgctcgcg cttgttgaaa tgcttgcgtc
     3781 cccgctgcac tacattgaac gccatggtaa atgcgggaat ggtgccggcc ggcatctggt
     3841 ccacctgcgg aatgggaatg ccatacaccg acacatccca ttttttcgag cgcttgagaa
     3901 tattgaaacg caccatcgag tgcatcagac gcaccagcgc tgccgccttg aaacccgcgc
     3961 cataacggcc gagtgcgcca ggcaatgtgg cggtggtgaa aaagctggcg gtttccttca
     4021 cccgctgcac cgacgtctca ctggacaatg ccccggtcaa cgccatcggc aatccggaat
     4081 atttgttcat gaaggtggcg acaaaggtgc cacgaattgc aaacg
//
LOCUS       DASUEG010000501         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2156155, whole genome shotgun sequence.
ACCESSION   DASUEG010000501 DASUEG010000000
VERSION     DASUEG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156155"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..443
                     /locus_tag="VFX11_09385"
     CDS             153..443
                     /locus_tag="VFX11_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4938875.1"
                     /translation="MPVDIETSPGLTQVKISGSCTIFEAAELRQLLLPVAAVTTPVVV
                     DLSAITDIDTAGLQVLLALQKQCALALFSKPSRAMQTALDLLNLNRCFSIEV"
     gene            455..2431
                     /locus_tag="VFX11_09390"
     CDS             455..2431
                     /locus_tag="VFX11_09390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX4938876.1"
                     /translation="MDLNESLGILVQEARELLADMEAALLAIEAQGVEPDRINALFRA
                     AHTIKGSAGLFGLDPIVSFTHLMESLLVDVRSGELALEPELAGLLFNCADHLGSLIDC
                     LETGGDLAACEPDLRAVLMDSLRDYWQVECPATAAAPALLPGSTDARTWQIQLRFAPD
                     LLAQGMDPQSFLLYLTNLGELISINTGTEALPALADLDPEICYLTFELELLTAASEAE
                     IAATFDFARDGSDIRIRCLDQPAASAVAPMPAPTTKTVTTAGTNTGPRNGRDSSLIKV
                     DSRKLDELIDAVGELVIRSASCHAHPVIRGNAALAELMESVGGLVEQIRDRALNMRMV
                     PIGEVFQRFPRVVRDQCQELGKRIDLQISGADTELDKSMVEKLTDPLLHIVRNAMDHG
                     IEPVADRVAAGKPEQGILRLNAYHQSGAVVIEISDDGRGLDTPKILAKAIERGLVAPE
                     AKLAERDIFNLIFAPGFSTADQVTDLSGRGVGMDVVRQNIEQLRGSIDIQSTQGVGTR
                     FQIQLPLTLAIIDGFEVAVGDSHFVLPLDMVVECLEFSASAGNDIFNLRGQPLPYLRL
                     AQQFAIDSTPEGRECLVVLQCGDQRAGLVVDRFVGELQAVIKPLGHLLGSMRGFSGST
                     ILGDGSVALLLDIPALVARAARHATAPLSLHGAT"
     gene            2479..>3451
                     /locus_tag="VFX11_09395"
     CDS             2479..>3451
                     /locus_tag="VFX11_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4938877.1"
                     /translation="MKWFLKLSLAKKLLTTFVTLALITAGVGAYGLVNIINVGGLMKA
                     MYVNNLVAIDNLSEAYSRYLVYTRAVTRAPTQNAEELAATEERMKGHWAKSTAALDEY
                     RNTVLSENEQQLLAKLDNEQKRYLQAVAQLFDLLKQGKKAEAADLSNNEMRLASYEIE
                     AAYAALIEDNNKQGSAADAAGDAAVKQMELVMGTMILVAMALAIGFGIFVTRIVTRQL
                     GGEPDYAGDVVRRIAEGDLTVRINLRKGDDSSLLAAMNNMLERLTDIIGQVTSSADAL
                     AAASEQVSASSGTLSQNATEQAASVEQTSASMEEIAATVAQNAENAEL"
BASE COUNT          688 a         1096 c         1059 g          608 t
ORIGIN      
        1 caattcataa tgacgcttac agaaagttga gatccccgac atccccgtga cccgggggtt
       61 tcccctcatc ccccctgagt aattccactc ttaacgcttt gcctgaaggg caaccagact
      121 tgtgtcatcc cggaatgaca cgagtgcgca ccatgcccgt cgatatcgaa accagcccag
      181 gcctgaccca ggtgaagatc agcggcagct gcaccatttt cgaagcggcc gagctgcgcc
      241 aattgctgct gccggtggct gccgttacca ccccggtggt ggtggacctg tcggccatta
      301 ccgatatcga caccgccgga ttgcaggtat tgctggcatt gcagaagcag tgtgccctgg
      361 ccctgttcag caaaccgtcc cgtgcaatgc agaccgccct ggacctgctc aatctcaacc
      421 gctgtttcag catcgaagtc tgaggaaatc ccccatggat ctgaatgaat cgctgggtat
      481 tctggtacag gaagcacgcg agctgctggc agacatggag gcggcactgc tggccatcga
      541 ggcccagggc gttgagcccg accgcattaa tgccctgttc cgcgcggccc ataccatcaa
      601 ggggtctgcc ggcctgttcg ggctggaccc gatcgtcagc ttcacccatt tgatggaaag
      661 cctgctggtg gacgtgcgca gcggcgagct ggcgctggag ccggaactgg cgggcctgct
      721 gttcaactgt gccgaccacc tgggcagcct gatcgattgc ctggaaaccg gcggcgacct
      781 ggcggcctgc gagccggacc tgcgggcggt gctgatggat tccctgcgcg attactggca
      841 ggtggaatgt ccggcaacgg ctgcggcccc ggcgctgcta cccggttcca ctgacgcccg
      901 cacctggcag atccagctgc gttttgcccc ggatctgctg gcccagggca tggacccgca
      961 atccttcctg ctgtacctga ccaacctggg tgaactgatt tccatcaaca ccggcaccga
     1021 agccctgccg gcgctggcag atctggaccc ggaaatctgc tacctgacct tcgaactgga
     1081 actgctgacc gcggccagcg aagccgagat cgccgccacg ttcgatttcg cccgcgatgg
     1141 cagcgacatc cgtatccgct gcctggatca acccgcggcg tcagccgttg caccgatgcc
     1201 ggcgcccacc accaaaaccg tcaccactgc aggcacgaac accggcccgc gcaacggccg
     1261 cgacagcagc ctgatcaagg tggactcacg caagctggac gaactgatcg acgccgtggg
     1321 cgaactggtg atccgcagtg ccagctgcca cgcccatcct gtgattcgcg gcaatgcggc
     1381 gctggctgaa ctgatggaaa gcgtcggtgg cctggtggaa cagatccgcg atcgcgccct
     1441 gaatatgcgc atggtgccca tcggcgaagt gttccagcgt ttcccccgcg tggtgcgtga
     1501 ccagtgtcag gaactgggca agcgcatcga cctgcagatc agcggtgccg acaccgaact
     1561 ggacaaatcc atggtggaaa aactcaccga tccgctgctg cacatcgtgc gcaacgcgat
     1621 ggatcacggc atcgagccgg tggcggatcg tgtggccgcc ggcaagccgg agcagggcat
     1681 attgcgcctg aatgcgtacc accagtccgg tgcggtggtg atcgaaatca gcgatgacgg
     1741 ccgtggcctg gacacgccga aaattctcgc caaggccatc gaacgcggcc tggtggcgcc
     1801 cgaggcgaaa ctggcggagc gcgacatctt caacctgatt ttcgcgccgg gtttttccac
     1861 cgccgatcag gttaccgatc tgtcgggccg tggtgtcggc atggacgtgg tgcggcagaa
     1921 catcgaacag ctgcgcggca gcatcgacat ccagagcaca cagggtgttg gcacccgttt
     1981 ccagatccag ttgccgctga cgctggccat catcgatggc tttgaagtcg ccgtgggcga
     2041 cagccacttc gtgctgccgc tggacatggt ggtggagtgt ctggaattca gcgccagcgc
     2101 cggcaatgac attttcaatc tgcgcggcca gccgttgcca tatttgcggc tggcgcaaca
     2161 gttcgccatc gactccacgc cggaaggccg tgaatgtctg gtggtgctgc agtgcggcga
     2221 ccagcgcgcc ggactggtgg tggaccgctt tgtcggcgaa ctgcaggccg tgatcaaacc
     2281 gctgggccat ctgctgggca gcatgcgtgg cttcagtggc tccaccatcc tgggggatgg
     2341 cagtgtggcc ctgctgctgg acattccggc gctggtcgcc cgcgctgccc gccatgcaac
     2401 ggcaccgttg tcgctgcacg gcgcaacctg atttctccaa cccatttgtg taatggaaaa
     2461 caacccgagg ttcttgcaat gaaatggttt ctcaaactca gcctggcgaa aaaactgctc
     2521 accaccttcg tcaccctggc gctgatcacg gccggggtgg gcgcatacgg cctggttaac
     2581 atcatcaacg tgggcggact catgaaggcg atgtatgtga acaacctggt ggcgatcgac
     2641 aacctgtcgg aagcctacag ccgctatctg gtgtacaccc gcgcggtgac ccgggcgccg
     2701 acgcagaatg ccgaggaact ggcggcgacc gaggagcgca tgaaggggca ctgggccaaa
     2761 tccactgctg cgctggacga ataccgcaac acagtcctga gcgagaacga gcagcaactg
     2821 ctggccaaac tggacaacga gcagaagcgc tatctgcagg ccgtggcgca gctattcgat
     2881 ctgctcaaac agggcaaaaa agccgaagcg gccgacctca gcaacaacga gatgcgcctg
     2941 gccagctatg aaatcgaggc cgcctacgcc gcactgatcg aagacaacaa caagcagggc
     3001 agcgcggccg atgccgctgg cgacgccgcc gtgaagcaga tggaactcgt gatgggcacc
     3061 atgatcctgg tggccatggc actggcaatt ggcttcggca ttttcgtcac ccgcatcgtc
     3121 acccgtcagc tgggtggtga accggattat gccggcgacg tggtgcgccg catcgccgaa
     3181 ggcgatctca ccgtgcgcat caacctgcgc aagggcgacg acagcagcct gctggccgcc
     3241 atgaacaaca tgctggaacg cctcaccgac atcatcggtc aggtcacctc ctcggcggat
     3301 gcgctggccg ctgcatccga gcaggtatcg gcttcgtccg gcactctcag ccagaacgcc
     3361 accgagcagg ctgccagcgt ggagcagacc agcgcctcga tggaggaaat cgccgccacc
     3421 gtggctcaga acgcggaaaa tgccgagctc a
//
LOCUS       DASUEG010000502         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1923780, whole genome shotgun sequence.
ACCESSION   DASUEG010000502 DASUEG010000000
VERSION     DASUEG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923780"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="mltB"
                     /locus_tag="VFX11_09400"
     CDS             <1..704
                     /gene="mltB"
                     /locus_tag="VFX11_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX4938878.1"
                     /translation="TLEKAEKEFGVSRYIIMAIIGVETRYGKHKGNYRVIDALATLAF
                     DYPPRAPFFRNELKEFLHLQNEAHIDLMTAKGSYAGAMGYPQFISSSYRKWAVDFDAD
                     QRIDLIDNPVDAIGSVASYFKAHGWQDGAPVVSLARYMGKEENEATLDKWVNQDLLPV
                     HTVAEFRKAGLEPDQTVADDQKATALKLEGAEGIEYWLGLNNFYVITRYNRSPLYALS
                     VFQLSEALRSKLNPT"
     gene            701..1642
                     /locus_tag="VFX11_09405"
     CDS             701..1642
                     /locus_tag="VFX11_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX4938879.1"
                     /translation="MTEFRIQGVTRCLIVLLALWLAACSSSPPARSGSSSGTGISSTP
                     YISFDDMKDSAPDETRDWSKIPDAVPRWEPRARYGNHSPYRVLGKTYTVLPNSVGYRE
                     TGIASWYGKKFSGRATSSQEPYDPYAMTAAHKRLPLPTYVRVTNLDNRRQIIVKVNDR
                     GPFHDNRIIDLSFAAAHKLGYANKGTARVLVEAIDTREAGREPPPPSSVLGNRPAPAA
                     AIPPMAAASPAPTPVTPAQIYLQVGAFADLNAAVRLQQQLIPLTQVPVGIFSQFENGR
                     SDVHRVRVGPISSEQDAQRIQQLIRNSRLGDPLLIRR"
     gene            1731..2888
                     /locus_tag="VFX11_09410"
     CDS             1731..2888
                     /locus_tag="VFX11_09410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX4938880.1"
                     /translation="MTQTRIALVLTFLLTVLLHVPAQAARMIPAAPTLAAKSWLLIDA
                     RTGHVITEYNADERVAPASLTKMLTSYIASERIKNGELKMTDLVPISEKAWRMGGSKM
                     YVHVGHQVKVEDLLRGIIIQSGNDASIAVAEYVAGSEDAFADLMNKTAQSLGMKASHF
                     ENATGWPSPNHYTTARDLAILAQAIVYNHPEDYALYAEKEFTYNNIRQPNRNTLLWND
                     PSVDGIKTGHTEEAGYCLVSSAKSSDIRLIAVVMGTRSEKERAAESLKLITYGFRFFE
                     TAKSYSAQTVLAEPEIWMGNVNTVKLGAAEDLYVTIPRGAAKDVTAELEVPEMLKAPV
                     TAGQQIGTIKLTLDGEVIANKPLVALQAVEEGGIFTQVWHWLVLLVKGLLS"
     gene            2914..3792
                     /locus_tag="VFX11_09415"
     CDS             2914..3792
                     /locus_tag="VFX11_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX4938881.1"
                     /translation="MAAQPANNPVAYLNGLYLPLEEARISPLDRGFLFADGVYEVIPC
                     YNGRLFRLEQHIERLIRSLEAILLPSPHTETEWIDLLNGLVQRNGGGHQSLYVQVTRG
                     APALRDHRFPDHCPATVFALSQTLEDPESRLLDAETGIRAMTCADTRWSHCDIKSVAL
                     LANVLLRHEARQAGCDEAILIRDGHVTEGATSNVFVVRNGELLTPPLGPHILDGITRD
                     LIIELARQHRMPLREAPIAETQLAQADEIWISSSTRGARPVVLLDGQPVGDGRPGALW
                     RTMAEHYAAFRRALMQ"
     gene            3849..4130
                     /locus_tag="VFX11_09420"
     CDS             3849..4130
                     /locus_tag="VFX11_09420"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX4938882.1"
                     /translation="MSKQPTGINEQIWEFPNTHDIKVLGLSHYPLVDVVIEVVKQHAP
                     DFDPGTIRTSPSRTGKYLSVTASVHFTSKEQLEALYLALHQREEVSQTL"
     gene            4208..>4758
                     /gene="lipB"
                     /locus_tag="VFX11_09425"
     CDS             4208..>4758
                     /gene="lipB"
                     /locus_tag="VFX11_09425"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX4938883.1"
                     /translation="MQALTNSRGSRTGDEIWLLQHPPVFTQGQAGKAEHLLAPGDIPV
                     VQVDRGGQVTYHGPGQLVAYLLVDLRRLRIGARELVTRIETSLVQTLAELDIEAAPRA
                     DAPGVYTQGRKIASLGLRIRHGCSFHGLALNVDMDLEPFHRINPCGYQGLQMAQVKEF
                     VNRPDFAAISARLADHLANNLGYT"
BASE COUNT         1009 a         1627 c         1344 g          778 t
ORIGIN      
        1 ccaccctgga aaaggccgaa aaggaatttg gcgtatcgcg ctacatcatc atggcgatca
       61 tcggtgtcga gacccgctac ggcaagcaca agggcaacta ccgggtgatc gacgcactcg
      121 ccaccctggc gttcgactat cctccgcgcg cgcccttctt tcgcaatgaa ctgaaagagt
      181 tcctgcacct gcagaacgaa gcccatattg atctgatgac cgcaaaaggt tcctacgctg
      241 gcgccatggg gtatccccag ttcatttcca gcagctatcg caagtgggca gtggatttcg
      301 acgccgacca gcgcatcgac ctgatcgaca atccggtgga cgccatcggc agcgtcgcca
      361 gctatttcaa ggcccacggc tggcaggacg gtgcgccggt ggtcagcctg gcccgttaca
      421 tgggcaagga agagaacgaa gccacgctgg acaaatgggt gaaccaggac ctgctgccgg
      481 tgcacacggt ggcggaattc cgcaaggccg ggctggaacc ggatcagact gttgcggatg
      541 accagaaggc cactgccctg aaactggaag gtgcggaggg tattgaatac tggctgggcc
      601 tgaacaactt ctacgtgata acccgctata accgcagtcc gctctacgcg ctgtcggtat
      661 tccagttgag cgaagccctc cggagcaagc tgaaccccac atgaccgaat ttcgcattca
      721 aggtgtgacg cgctgcctga tcgtgttgct ggcactgtgg ctggccgcgt gcagcagcag
      781 cccgccagca cgctccggta gcagcagcgg taccggtatc agcagcacac cttatatttc
      841 cttcgacgac atgaaggaca gcgcaccgga cgaaacccgc gactggtcga agatccccga
      901 cgcggtcccc cgatgggaac cccgcgcccg ctatggcaac cactcaccct accgggtact
      961 gggcaagacc tacaccgtgc tgcccaacag cgtcggttac cgcgaaaccg gcattgcctc
     1021 gtggtacggc aagaaattct ccggccgcgc cacctccagc caggaaccgt atgaccccta
     1081 cgccatgacg gccgcccaca agcggttgcc actgcccacc tatgtgcgcg tcaccaacct
     1141 ggacaaccgg cgccagatca tcgtcaaggt gaatgatcgc ggccccttcc acgacaaccg
     1201 catcatcgac ctgtccttcg cggccgccca caagctgggc tatgccaaca agggcaccgc
     1261 ccgggtgctg gtagaagcca tcgacacgcg ggaagcaggg cgcgaaccgc caccgccttc
     1321 cagtgtgctg ggcaacaggc ccgcgccggc agccgcaata ccgcccatgg ccgccgcatc
     1381 gccggcgccc actccggtga caccggcgca aatctacctg caggtcggcg cctttgccga
     1441 tctcaacgcg gcagtgcgcc tgcagcagca gctgattccc cttacccagg tgccggtagg
     1501 catcttctcc cagttcgaaa acggccgcag cgatgtacac cgcgtacggg tgggtcccat
     1561 cagttcggaa caggacgcac agcgcattca acaactgatc cgcaattccc gcctcggcga
     1621 cccgctgctg atccgccgtt aaggtcacat tcaggcggca ctgtgctacc atcccccgac
     1681 ttgacgcaca aatcgcaaaa tcccttattt tcccagataa aaccgacatc atgacccaga
     1741 ctcgcattgc actcgtcctc accttcctgc tcactgtcct gctgcatgtt ccggcccagg
     1801 ccgcgcgcat gattcccgcc gcgccgacac tggccgccaa atcctggctg ctgatcgacg
     1861 cccgcacggg ccatgtcatt accgaataca acgcggacga acgagtcgcc cccgccagcc
     1921 tcaccaagat gctcaccagc tatatcgcat cggagcgcat caaaaacggc gagctgaaaa
     1981 tgacggatct ggtgcccatc agcgaaaaag cctggcgcat gggcggttcc aagatgtatg
     2041 tgcacgtcgg ccaccaggtc aaagtggaag atctgctgcg gggcatcatc atccagtccg
     2101 gcaatgatgc cagcatcgcc gttgccgaat atgtggccgg cagcgaagat gccttcgccg
     2161 acctcatgaa caaaacggcc cagagcctgg gcatgaaagc cagccatttc gaaaacgcca
     2221 ccggctggcc atcgccgaat cactacacca ccgcccgcga tctggcgatc ctggcccagg
     2281 ccatcgtcta caaccatccg gaagactatg cgctgtatgc agaaaaggaa ttcacctaca
     2341 acaatatccg ccagcccaac cgcaacacgc tactgtggaa cgatcccagc gtcgacggca
     2401 tcaagaccgg ccacaccgag gaagcgggct actgcctggt gtcgtcggcc aaatccagcg
     2461 acatccgcct gatcgcggtg gtgatgggca cccgctccga gaaggaacgg gcggccgaga
     2521 gcctgaaact gatcacctac ggtttccgtt tcttcgaaac agcgaaaagc tacagcgccc
     2581 agaccgtgct ggccgagccg gaaatctgga tgggcaacgt caacacggtg aaactgggtg
     2641 cggcggaaga cctgtatgtc acgattcccc gtggcgccgc caaggacgtg accgccgaac
     2701 tggaagtgcc cgagatgctg aaggcacccg tcacagccgg ccagcagatc ggcaccatca
     2761 agctcaccct ggacggcgaa gtcatcgcca acaaaccgct ggtggcactg caggcggtgg
     2821 aggaaggcgg tatcttcacc caggtatggc actggctggt actgctggta aaaggcctgc
     2881 tcagctgagg cccggctcat tcaaggaaac accatggccg cgcagcccgc gaacaatcct
     2941 gtcgcctacc tgaacggcct gtacctgccc ctggaagaag cccgcatctc acctctggat
     3001 cgcgggtttc tgttcgctga cggcgtgtac gaagtgattc cctgctacaa cggcaggctg
     3061 ttccggctgg agcagcacat cgaacgcctg atccgcagcc tggaagccat cctgctgcca
     3121 tccccgcata ccgaaaccga atggattgac ctgctgaacg gcctggtgca acgcaacggc
     3181 ggcggccacc agtcgctgta cgtgcaggtc acccggggcg cccctgcact ccgggaccat
     3241 cgctttcccg accactgccc cgccaccgtg ttcgccctgt cgcaaacgct ggaagaccct
     3301 gagtcacgcc tgctggacgc ggaaaccggc attcgcgcca tgacctgtgc cgatacccgc
     3361 tggagccatt gcgacatcaa gtcggtggcg ctgctggcca acgtgctgct gcgccacgaa
     3421 gcccggcagg caggctgtga cgaggccatc ctgatccgtg acggccatgt caccgaaggt
     3481 gccaccagca atgtctttgt ggtgcggaac ggtgaactgc tcaccccgcc actggggccg
     3541 catattctgg acggcatcac ccgggatctg attatcgaac tggcccgtca gcaccgcatg
     3601 ccgctgcggg aagcgccgat agcagaaacg cagctggcgc aggcggacga aatctggatc
     3661 agcagctcca cccggggcgc ccggcccgtg gtgctgctgg atggccagcc ggtgggcgat
     3721 ggccgccccg gcgcgctgtg gcgtacaatg gcggaacact atgcagcgtt ccgacgggcg
     3781 ctgatgcagt aaaccgaacg cggacagccc tccgcagccg ttctccgcaa gagccatcca
     3841 gagacaccat gagcaaacag cccaccggca tcaacgaaca aatctgggaa tttcccaaca
     3901 cccacgacat caaggtactg ggcctgtccc actacccgct ggtggacgtg gtgatcgaag
     3961 tcgtcaaaca gcacgcgccg gacttcgacc ccggcaccat ccgcacctcc cccagccgca
     4021 ccggcaaata tctgtcggtg accgcctcgg tgcacttcac cagcaaggaa cagctggaag
     4081 ccctgtacct ggccctgcac cagcgcgaag aggtctcgca aaccctgtga ccattccggc
     4141 aaccgacacc cgcagcgtgc tgttccgcca gctgggtctg gtggactacg tcaccacctg
     4201 gcacgccatg caggctctca ccaacagccg cggcagccgc accggcgacg aaatctggct
     4261 gctgcaacat ccgccggtat tcacccaggg tcaggctggc aaggccgagc acctgctggc
     4321 ccccggcgac atccctgtgg tgcaggtgga tcgcggtggc caggtcacct accatggccc
     4381 cggccagctg gtggcctatc tgctggtaga cctgcgccgc ctgcgcatcg gcgcccgcga
     4441 actggtcacc cgcatcgaaa ccagtctggt gcagaccctg gcagaactgg atattgaagc
     4501 cgccccccgt gccgacgcgc ccggcgttta cacccagggc cgcaagatcg cctcgctggg
     4561 cctgcgcatc cgccacggct gctcctttca cggtctggcc ctcaacgtcg acatggacct
     4621 ggaacccttc caccgtatca acccctgcgg ctatcagggc ttgcagatgg cgcaggtgaa
     4681 agaatttgta aaccggccgg atttcgccgc catcagcgcc cgtctggcgg atcatctggc
     4741 caacaatctg ggatatac
//
LOCUS       DASUEG010000503         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1156014, whole genome shotgun sequence.
ACCESSION   DASUEG010000503 DASUEG010000000
VERSION     DASUEG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156014"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..508
                     /locus_tag="VFX11_09430"
     CDS             92..508
                     /locus_tag="VFX11_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938884.1"
                     /translation="MTQQELLARLRTYIGGALGNAAADEIERLTQQVAWWKQAASTSR
                     DDAHAWQIAANAKFEQPTIERIAQQLAEHGLTIVSGKDAEKIKRCECDTIKKCIDIVE
                     RYKVPVGNSSAGELACEWTMDALREIRDEMKGLCDG"
     gene            625..951
                     /locus_tag="VFX11_09435"
     CDS             625..951
                     /locus_tag="VFX11_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938885.1"
                     /translation="MTDRELLELAALAAGIVGTYDCGVIWYKNPFGDDAGFNPLNNDG
                     DAFRLAVKLRIGVGDRETTVTAFHRQGSTSAIHIEEIGDPYAATRRAILRAAAEIGKQ
                     IKEGGE"
     gene            948..1529
                     /locus_tag="VFX11_09440"
     CDS             948..1529
                     /locus_tag="VFX11_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HEX4938886.1"
                     /translation="MSEKLKPCPFCGGEAQHAGLLAVCKKCGGAAWDADSWNRRAAPD
                     APAVQASICLALGLGDDASVDDALFAIGVLKDRANVPAVSAEPVMYRYKMACSAYPEG
                     VWVDELCADMTLLETQALYTHPPAPAEAWQPIDTAPKDGSLFMAWRDHCTYPIVAQYV
                     ASHDWIEEYPSGNHLYHITHWMPLPTPPNGGES"
     gene            1526..2191
                     /locus_tag="VFX11_09445"
     CDS             1526..2191
                     /locus_tag="VFX11_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938887.1"
                     /translation="MKKCTQERFLNDVARHEMQILHEDGLYRHVRFKRPDSGCMHFDL
                     ITYPGYLVFSGDMGCYVFSRLTDMFEFFRTDRRDYPDGDQLRINLGYWSEKLQAVDGN
                     RDQGSAKEFSEEKFNRAVVGDLIAWIRSYSYQTTKDERRRLWDAVMCEVIGEDSDSGG
                     YRKQAAVHDFYHDVNSRVGRFSFQDFWEHDVTDYTFRFVWCCYALAWGIAKYDDNKGH
                     HNG"
     gene            2184..>2543
                     /locus_tag="VFX11_09450"
     CDS             2184..>2543
                     /locus_tag="VFX11_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938888.1"
                     /translation="MDDIKLPEMALPEPTLMPRSEDVYAYTTYQMHEYADAKARAAVL
                     ADRERRRPCSPEQAIAAWNAKPAAVKESLTPEQEREAFEAWYERDGVKVRKSAVCTSY
                     YRPSIQDAWEAWQARAAL"
BASE COUNT          599 a          638 c          810 g          496 t
ORIGIN      
        1 ctgactcatg gatgacaaca cgactttcgt aatcatcatg gtggcctggt tgattttcat
       61 ggcctggata atcaaggact gagggtaaac gatgacacaa caagaactgt tggcccggct
      121 gcgcacgtac atcggcggcg cgcttggcaa tgctgctgcc gatgagatcg agcggctgac
      181 acagcaggtt gcatggtgga agcaagccgc aagcacaagc agagatgacg cgcacgcatg
      241 gcaaattgcc gcaaatgcta agtttgagca gccgacgatt gagcggatag cgcagcagct
      301 cgcagaacat gggctgacga ttgtcagtgg gaaggacgcg gagaagatta aacggtgcga
      361 gtgcgacact attaaaaagt gcattgatat cgtcgagcgc tataaggttc cggtcggcaa
      421 cagttcagcc ggcgaactgg cttgcgaatg gacgatggac gcgctgcggg aaatacgcga
      481 tgaaatgaag ggactttgcg atggataaaa accttggact gtttctaggt gccgctatcg
      541 gggcaatcat tggctgttgg ctggcggttt tgatcgaaag ctggcttgaa tggcggcgcg
      601 tgagaaagca gaaggggatc aaccatgact gaccgcgaac tactggaact ggccgcgctg
      661 gcggctggga tcgtcggaac gtatgactgt ggcgtgatct ggtacaaaaa tccgtttggg
      721 gatgacgcag gatttaaccc actgaacaac gacggcgatg ctttcaggct ggcggtgaag
      781 ctgaggatcg gtgtcggtga tcgtgaaacg acagtaacgg cttttcacag acaaggctct
      841 acatcggcga tccacattga ggagataggc gacccctacg ccgccacgcg ccgggcgatt
      901 ttacgggcgg cggctgagat cggcaaacag attaaggagg gtggggaatg agcgaaaaac
      961 tgaagccgtg cccgttttgc gggggcgaag cacagcatgc cggtctgctt gctgtttgca
     1021 aaaaatgtgg tggcgctgct tgggacgccg attcttggaa ccgccgcgcc gcgcccgatg
     1081 cgccggcagt gcaggccagc atctgccttg cgctggggct tggggatgat gcgagcgttg
     1141 acgatgcgct gtttgcgata ggcgtcctga aagacagggc gaacgtgccg gcagtgagcg
     1201 cggagccggt tatgtatcgg tacaaaatgg catgcagcgc gtacccggaa ggcgtgtggg
     1261 tggacgaact gtgcgcagac atgactctgc tcgaaacgca agcgctttac acccacccac
     1321 ccgcgcctgc agaggcgtgg cagccgattg atacggcgcc gaaggatggc agtctgttta
     1381 tggcgtggcg agatcactgc acatacccaa tcgttgcgca gtatgtggcc agtcatgact
     1441 ggatagagga ataccccagc gggaatcatc tgtaccacat aacccactgg atgccactgc
     1501 cgacgccgcc gaatgggggt gaatcatgaa aaaatgcaca caagagcgtt ttcttaatga
     1561 cgtggcgcgc catgaaatgc agatactgca cgaggatggc ttgtaccgtc atgtccggtt
     1621 caagcggcct gactctggct gcatgcactt cgacctgatt acctatccag gctatctggt
     1681 cttctcgggc gatatgggct gttacgtgtt ctcgcgattg acggatatgt tcgagttctt
     1741 tcggacggat cggcgcgatt atccagatgg cgatcagctt cggatcaacc ttgggtactg
     1801 gtctgagaag ctgcaggcgg tggacggcaa tcgagaccaa gggtctgcca aggaattcag
     1861 cgaggaaaag ttcaatcgcg cggtggtggg cgatttgatc gcgtggatcc gctcgtattc
     1921 ctaccagacc accaaggatg agcggcgaag actgtgggat gccgtcatgt gcgaagtgat
     1981 tggcgaagac agtgattccg gcgggtaccg caaacaagcg gccgtccacg acttctatca
     2041 cgacgtcaat agcagggtcg gccgcttcag ctttcaagac ttttgggagc atgacgttac
     2101 cgactacacg ttccgctttg tctggtgctg ctacgcgctg gcatggggaa ttgccaagta
     2161 cgacgacaat aaaggacacc acaatggatg acatcaaact gccggaaatg gcattgcccg
     2221 agccgacact gatgccgcga tcggaagatg tgtacgccta caccacttac cagatgcacg
     2281 aatacgccga cgcaaaggcc cgcgccgcag tgctggcgga tcgggagcgg aggcgcccat
     2341 gcagcccgga gcaagcgatt gctgcatgga atgccaagcc cgcagccgtc aaggaatcct
     2401 tgacacctga gcaggagcgg gaagcgttcg aggcgtggta cgagagggat ggcgtcaagg
     2461 tgcgtaaatc cgccgtatgc acctcatact accgccccag cattcaagac gcatgggaag
     2521 catggcaagc ccgcgcagca ctg
//
LOCUS       DASUEG010000504         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1328736, whole genome shotgun sequence.
ACCESSION   DASUEG010000504 DASUEG010000000
VERSION     DASUEG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328736"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2922)
                     /locus_tag="VFX11_09455"
     CDS             complement(<1..2922)
                     /locus_tag="VFX11_09455"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4938889.1"
                     /translation="MSDTKSSDKKFSLSKAVVVALPLLAASGMAATARNSSHTSNRRG
                     GNRASVVHVTAQDEAAKKVGGDKRNAFSAFGRSSGLPKSAKAVVDNSALAFLRENGLL
                     QKQADSSAQLSGELIGRKRFASESPFAHCHISWGTYYTNTWTSLNTAKCASYAGTWHS
                     ASTKPAVLSSSVINKTATSFDVSLTMSENSTVYAVLLLTTQPAPTSAQVIAGTDGSGG
                     AAVQTKSQAMNPSGTLSFTGLSSSASYMVYVVGRDASLEVTTTPKQVNPAYETGMIAD
                     TASWQFPKVRSAPNGDFYLARSDGSNIRFLKWNGAGFTLHTTLTPSSVPGRTNGGWGN
                     PDSVDYEVDASGNIHVIFSAGTTNWAVDYDPFYAVYNGTSWTYTNIGETGYTIFDADL
                     SLDPNGKVHTSFQGSAAGNTIRYATNKSGAWAMTTIASTSGGTDELFDSYVLADSAGT
                     ATVIYRREDDQNIGTDNYYIASSTDNFVAKTLVLDGKTDGKMYLVAGAVMDGDGKIHY
                     AYNNRTDHRSYYRTNASGSWVQQEITNATYSSVDTLDIERIGSTYYFASRSGTTYFLT
                     SYDGSNWVDGFDFSLTGYLNDRFAISDVADRAMIVSENSTNYTIHYHTTTIAGYITAA
                     VANSAPTDIALSASSVNQSAGTNATVGTLSTTDADVSDTHTYSLVAGTGDTNNASFNI
                     SGSTLRANNSSALAAGTYSVRVQTSDGSLTYEEAFTITVVDNVAPTVSSVSVPANDTY
                     GPGESLSFTVNTGENITVNTGGGTPRLTLTIGSTTRYATYVSGSGTTALVFSYTVQSG
                     DFDGDGVALGASVDLNGGTLRDAASNNLTLTLNSIGSLTSVLVDAVAPNAPSTPDMTA
                     GTDSGSSSTDNITSDTTPTFTGTAEANATVALTSSVSGSLGSTTADGSGNWSFTPGAA
                     LSAGAHTITATATDASNNTSSASSGLAIVIDTAAASGHSVNLDDSAINASEAGSTSFT
                     FA"
     gene            complement(2940..4139)
                     /locus_tag="VFX11_09460"
     CDS             complement(2940..4139)
                     /locus_tag="VFX11_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX4938890.1"
                     /translation="MQDYEHLDFGCTYSANLPAVLKQLNIALAFTSYQAGRLMLVRSD
                     GDALDVNFKSFPRPMGLSATDRGLTLGVFTQVHHFAREDGLLEKIKQPLQRIEDDITA
                     PRIKPKEAGNAVPEAVAASDADETEQQRQIRAYEDSLYAPVDARVDACFIARSTHHTG
                     MINIHDIDWGDDGLWAVNSSFSCLCTLEPDYSFVPRWKPHFISELVPEDRCHLNGMAL
                     KDGKPAYVTTFSKFNQTGMWRKHAEFDGTLMDVATNEVLLDGLAMPHSPRWHDGKVYF
                     CNSGHGQICRFDPLTHKLDILAEVPGFTRGMAFYGPILLVGLSKVRQADVTRAAPLSE
                     KYGETFSGIWLFNLQDGREIGHIRFTGNVDQIYDVAVIPDCHFPEVIEPTHPRMRNHF
                     CHPELQA"
BASE COUNT          830 a         1226 c         1262 g          887 t
ORIGIN      
        1 ggcgaaagtg aaactggtgg agccggcttc actggcattg atggcgctgt catccagatt
       61 gacgctgtgg ccgctggcag ccgccgtgtc gatcacaatg gccagacccg acgaagcgga
      121 cgacgtgttg ttcgacgcat ccgttgccgt agccgtaatc gtgtgcgcgc cggcgctcaa
      181 cgctgcgccc ggtgtgaaac tccagttgcc ggaaccatcg gccgtagtgg agcccaaact
      241 gcccgacacg ctcgacgtca gcgccacggt ggcattggct tccgccgtgc cggtaaacgt
      301 gggcgtggtg tcggaggtga tgttgtcggt gctggaactg ccggaatcgg tgcccgctgt
      361 catgtccggt gtgctgggcg cgtttggcgc aacagcatcc accagcaccg atgtcaggct
      421 gccgatggag ttcagcgtaa gcgtaagatt gttgctggcg gcatcgcgca gcgtgccacc
      481 attgagatcc acactggcgc ccagtgcaac gccgtcgcca tcgaagtcac cactctgcac
      541 ggtatagctg aataccagcg ccgtggtgcc actgcccgat acgtacgtcg cgtaacgcgt
      601 ggtagaaccg atggtcagcg tcaggcgtgg cgtgccgccg ccggtattca ccgtaatgtt
      661 ttcgcctgtg ttgaccgtaa aggacaggct ttcccccggc ccgtaggtat cgttcgccgg
      721 cacactcacg gaactgaccg tgggcgccac gttatccacc accgtaatgg tgaatgcttc
      781 ctcataggtg agactgccgt cgctggtctg cacgcgcacg gaataggtgc ccgctgccag
      841 cgctgaggaa ttgttcgcgc gcaaggtgct gccgctgata ttgaagctgg cattgttggt
      901 gtcgccggtg ccggccacca gggaataggt gtgagtatcg ctcacgtccg catcggtggt
      961 gctgagcgtg cccacggtgg cgttggtgcc ggcgctctga ttcacgctgc tggcactcag
     1021 cgcgatatcg gtaggcgcgc tgttggccac tgccgccgtg atgtaaccgg caatcgtcgt
     1081 ggtgtggtaa tgaatcgtgt aattggtgct gttctccgac acgatcatgg cgcggtcggc
     1141 cacatcgctg atggcaaagc ggtcgttcag atagcccgtc aggctgaaat caaaaccgtc
     1201 cacccagtta ctgccatcgt aggatgtcag gaaatacgta gtgccacttc gactggcaaa
     1261 atagtaggtg gaaccgatac gctcaatgtc gagcgtatca acgctgctgt aagtggcgtt
     1321 ggtaatttcc tgttgcaccc agctgccgga cgcgttggtg cggtaatagg agcggtgatc
     1381 cgttctgttg ttgtaagcgt aatgaatctt gccgtcgcca tccatcacag caccggctac
     1441 cagatacatc ttgccgtcag tcttaccgtc cagcacaagt gtcttggcga caaaattgtc
     1501 cgtcgacgat gcgatatagt aattgtccgt gccgatattc tgatcgtcct cgcggcggta
     1561 gatcaccgtg gcggtacccg cgctgtcggc cagtacatag gagtcgaaca gttcatcggt
     1621 gccaccactg gtactcgcga tcgtggtcat cgcccaggcg cccgacttgt tggtggcgta
     1681 gcgaatggta ttgccggcag cagaaccctg gaagctcgtg tgcaccttgc cattgggatc
     1741 aagcgacaga tccgcatcga atatcgtgta gccggtctca ccgatattgg tgtaggtcca
     1801 gctggtgcca ttgtaaacgg catagaaagg atcgtaatcc accgcccagt tggtggtacc
     1861 ggcggaaaaa atcacgtgaa tattgcccga cgcatccact tcgtagtcga cactgtccgg
     1921 attgccccaa ccgccattgg tgcggcccgg cacggatgaa ggcgtcagcg tggtgtgcag
     1981 cgtgaaacca gcaccattcc atttgaggaa ccggatgttg ctgccatcag aacgggccag
     2041 atagaaatcg ccattgggcg ctgagcgcac tttcgggaac tgccagctgg cggtgtcagc
     2101 aatcatgccg gtttcatagg ctggattcac ctgcttgggc gtggtggtaa cttccagact
     2161 ggcgtcacga cccaccacat acaccatgta cgacgcgctg gaactcaacc cggtaaacga
     2221 cagcgtgcca ctgggattca tcgcctgcga tttggtttgc acagcggccc cgcccgaacc
     2281 gtcggtgcct gcaatcacct gcgcgctggt gggtgccggc tgcgtggtca gcaacagcac
     2341 ggcgtagacg gtgctgtttt cgctcatggt caggctgacg tcgaaactgg tggccgtctt
     2401 gttgatgaca gacgaactca gtaccgccgg cttcgtggaa gcggaatgcc aggtgcctgc
     2461 ataactggcg cacttggccg tgttcaggct ggtccaggtg ttggtgtaat aggtgcccca
     2521 gctgatatga cagtgcgcaa acgggctttc actggcaaag cgcttgcgtc cgatcaactc
     2581 gccggacaac tgtgccgaac tgtccgcctg cttctgcaac aatccattct cgcgcagaaa
     2641 tgccagcgcg ctgttgtcca cgactgcctt ggctgatttc ggcagtccac tgctgcgccc
     2701 gaacgccgag aacgcgttac gcttgtcccc acccaccttc ttcgccgctt catcctgcgc
     2761 cgtcacgtgc accacgctgg cccggttacc accccgccgg ttgctggtat gactgctatt
     2821 gcgcgcagta gcggccatgc ccgacgccgc cagcaagggc agcgccacga caacagcttt
     2881 ggacagactg aattttttat cgctggactt ggtgtcagac atagtgatca ctcaacaaat
     2941 caggcttgca gttcagggtg acaaaaatga ttgcgcatcc ggggatgcgt gggctcgata
     3001 acttcaggaa aatggcagtc cggaatcacc gccacgtcgt aaatctggtc gacattgccg
     3061 gtgaaccgga tgtggccgat ctctctgccg tcttgcagat tgaacagcca gataccggaa
     3121 aaggtttcac cgtatttttc agacagcggc gccgccctgg tcacatcggc ctgccgtact
     3181 ttcgacagac ccaccagcag gatcgggcca tagaacgcca tgccacgggt aaagcccggc
     3241 acttcggcga ggatgtccag cttgtgcgtc agcggatcga aacggcagat ctggccgtgg
     3301 ccggaattgc agaaatatac tttgccatcg tgccagcgcg gtgaatgggg catcgccagt
     3361 ccatccagca gcacttcatt ggtggcgacg tccatcagcg tgccgtcgaa ctcggcgtgc
     3421 ttgcgccaca tgccggtctg gttgaacttg gaaaaggtgg tcacgtaggc gggtttgccg
     3481 tctttcagcg ccataccgtt cagatggcag cgatcctcgg gcaccagctc ggaaataaaa
     3541 tgcggcttcc agcgcggcac aaaactgtaa tcgggctcca gcgtgcacag gcaggaaaag
     3601 ctggagttca ccgcccacag gccgtcgtca ccccagtcga tatcgtgaat gttgatcatg
     3661 ccggtgtgat gggtggagcg cgcaataaaa caggcatcca cccgcgcgtc caccggcgca
     3721 tacaggctgt cctcgtaggc gcggatctgg cgctgctgct cggtttcgtc tgcgtcgctg
     3781 gctgcaacgg cttcagggac agcattaccg gcttccttcg gcttgatgcg cggcgccgta
     3841 atgtcatcct cgatccgctg caggggctgc ttgatctttt ccagcaggcc atcctcgcgc
     3901 gcgaagtgat gtacctgcgt gaacacgccc agcgtcaatc cacggtcggt ggccgacaaa
     3961 cccatggggc ggggaaaact tttgaaattc acatccagcg cgtcaccgtc gctgcgcacc
     4021 agcatcaggc ggccggcctg atacgacgta aacgccagtg caatattcaa ttgtttcaga
     4081 accgccggca gattggcgga ataggtgcag ccgaaatcca gatgctcata gtcctgcatt
     4141 cagctctcca gacagggcgg gcgatgtgat ggatacgggc atcagcaccg gcgattgcgg
     4201 cggcg
//
LOCUS       DASUEG010000505         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_517561, whole genome shotgun sequence.
ACCESSION   DASUEG010000505 DASUEG010000000
VERSION     DASUEG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517561"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VFX11_09465"
     CDS             <1..677
                     /locus_tag="VFX11_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938891.1"
                     /translation="YNAHQDESKQAQWRADKWMIGGTLLMGTGVLGIFGLPLFFYGLW
                     LQMQAAKAGLSVRPLIVTLIGYLVIIDSSLNSLGWAMDLFANHSLINRVLTAGWGAVF
                     DGGYFWHYNELKMGPYAIGGAGAPGEKAWEISLILACFCPRLAAAIGLLQMKRWGQQW
                     LIVTCWMGLIVWIGYNLNMTFYADVRYANITFPVWGWWVYDIFYITPFLSLPYLHTVN
                     REIFTD"
     gene            725..1162
                     /locus_tag="VFX11_09470"
     CDS             725..1162
                     /locus_tag="VFX11_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938892.1"
                     /translation="MKNTSENSLPVGTTEHFSNMHKWLQRGLFVCLVALVLEASLSLP
                     GLLIWFGWPTLSITEVCDELMKVRWSDDKAACLVPHPLYGANEGEGRTNGPAATAQDE
                     WGIQPRPLYKRINFRDLVKFRDERLAREAAAMSATPLPAPAIE"
     gene            1202..2449
                     /locus_tag="VFX11_09475"
     CDS             1202..2449
                     /locus_tag="VFX11_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4938893.1"
                     /translation="MSIAEAGKMKKPAHVPDELVFEFDYNNDPEYCRDPHARTVDLVK
                     KTPPIFWTPYNGGHWMFMSYKAVSEALIDYNKFSSEHFSPEAFAAMMSELPEEERIPA
                     PVPICIDPPLHTKLRQPLFSTFSPKSVAAMEEKIRGLAERLVDNIASKGQCEFQHAVS
                     DIYPVEIFLSMFGLPVEKEREYRDLAKKHLSVISPDLSENMLMMKAIADVLRDTIIDR
                     RENPREDLISRLWSLKLEGEPMTLELMLSYCVILFIAGLDTVVNALGFGVRHLATDPR
                     LQRELRENPELIPKATEEILRRYAFVAPIRMLKSDKEFHGIKFAQGERVMLFIPGASI
                     DEAAYPDATHFDLRREKLNHLAFGAGPHHCLGAHLARMELKIMYETLLSKLSEFRLDP
                     NNPPAFHGSIIAGPTTLHLVWDV"
BASE COUNT          636 a          746 c          707 g          574 t
ORIGIN      
        1 cttacaacgc acaccaggat gaaagcaaac aggcccagtg gcgcgcagac aagtggatga
       61 ttggcggcac acttctcatg ggaacgggcg tccttggcat attcggtttg ccactgttct
      121 tctacggcct gtggttgcaa atgcaggctg ccaaagcggg cctgtcggtc cggccgctga
      181 tcgtcaccct gatcgggtat ctggtgatca tcgattcctc gctcaacagc ctggggtggg
      241 caatggatct gttcgcgaac cattccctga tcaaccgggt tctcacggcc ggctggggcg
      301 cagtttttga cggcggctac ttctggcact acaacgaact caaaatgggg ccctacgcca
      361 ttggcggtgc cggtgcgccc ggcgaaaaag cctgggaaat atcattgatc ctggcatgct
      421 tctgcccccg tctggcggcg gcaatcggcc tgctgcagat gaagcgctgg ggacagcagt
      481 ggctgattgt cacctgctgg atgggcctga tcgtgtggat cggctacaac ctcaacatga
      541 cgttctacgc tgacgtacgc tacgccaaca tcacgttccc ggtatgggga tggtgggtct
      601 atgacatttt ctacatcaca ccctttcttt ctctgccata cctgcacacg gtgaaccggg
      661 aaattttcac agactgatac gatgtccgcc gaatcagacg tcaattattc aggagccgcc
      721 atcaatgaaa aataccagcg aaaacagttt gcctgtgggc acgactgaac acttctccaa
      781 catgcacaaa tggctgcagc gcggattatt cgtttgcctc gtggcgttgg tactggaagc
      841 ttcgctcagc ctgcccggcc tgctgatctg gtttggctgg ccgaccctgt cgattaccga
      901 agtatgcgac gaactgatga aagttcgctg gtcagatgac aaggcagcat gtctggtgcc
      961 gcacccactc tatggcgcca atgaaggcga aggtcgcacc aacgggcccg ccgcgactgc
     1021 gcaggatgaa tggggtatcc aacccaggcc gttgtacaag cgcatcaatt ttcgtgacct
     1081 tgtcaaattc cgggacgagc ggctggcccg tgaagcagcc gccatgagcg ccactccttt
     1141 gccagctccc gcaatcgaat aacagatttc aaacaatcgc caagtcgata aggacagtat
     1201 catgagcatt gcagaagcag gaaaaatgaa aaagccagcc catgttccgg acgaactggt
     1261 cttcgaattt gattacaaca acgatccaga atattgcagg gacccgcacg cccgcacggt
     1321 ggatctcgtt aaaaaaactc caccgatttt ctggacacct tacaacggcg gccactggat
     1381 gttcatgtcc tacaaagccg tttcagaagc gcttatcgac tacaataaat tttccagcga
     1441 gcatttctcg ccggaagcct ttgcagcgat gatgtccgag ttaccggaag aggagcggat
     1501 acccgcaccg gttcccatct gtatcgatcc gcctttgcac acgaaactgc gacaaccgct
     1561 gttttccaca ttctcgccaa aatccgttgc cgccatggaa gaaaaaatcc gcgggcttgc
     1621 cgaacgcctg gttgacaaca tcgccagcaa gggtcaatgc gaattccagc atgcagtgtc
     1681 cgatatttat cccgtcgaaa ttttcctgtc catgttcggt ctgccggttg agaaggaaag
     1741 ggaataccgg gatctggcaa agaagcatct ttctgtcata tcgccggacc tgtcggaaaa
     1801 catgctgatg atgaaagcca tcgccgacgt tctgcgcgat acgatcattg accggcggga
     1861 aaaccccagg gaagacctga tcagcaggct gtggtcgctg aaactggaag gcgagcccat
     1921 gacgctggag ctcatgctga gctactgcgt catcctgttt attgccgggc ttgatactgt
     1981 tgtgaacgcg ctcggatttg gtgtccggca tctggcaacc gatccgcgcc tgcagcgcga
     2041 actgcgtgaa aacccggaac tgattccaaa agcaaccgaa gaaatcctcc gccggtatgc
     2101 gtttgtggcg cccattcgca tgctgaaaag cgacaaggaa tttcatggca tcaaattcgc
     2161 gcaaggcgaa agggtcatgc tgtttattcc gggcgcgagc attgatgaag ctgcctatcc
     2221 tgatgccacc catttcgatc tgaggcggga aaaactgaat cacctggcat tcggcgccgg
     2281 gccccaccat tgcctgggcg cccatctggc acggatggag ctgaagatca tgtatgaaac
     2341 ccttctttca aagttgtccg aatttcgcct ggaccccaat aatccccccg cgtttcacgg
     2401 cagcatcatc gccgggccaa caacacttca tctggtttgg gacgtgtagc gttcaggtag
     2461 atcaacttac ttaacagatg gtgaatgaaa tggaaagcaa tacccagaaa acagagagct
     2521 caacgaattt aactgcgccc gcaaccgtag atggaagctg gagcgtcacc attcacggcc
     2581 ccaccggccc tcaagcgacg acgctggact tgataacagt caacggtgta ttgaccggca
     2641 cccaatccgc gatgggacag ctg
//
LOCUS       DASUEG010000506         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1404237, whole genome shotgun sequence.
ACCESSION   DASUEG010000506 DASUEG010000000
VERSION     DASUEG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404237"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX11_09480"
     CDS             <1..189
                     /locus_tag="VFX11_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEX4938894.1"
                     /translation="DKLRPGDILGALTGDAGIPGAMVGKIDIFDIQAYVAVDRDVASQ
                     AMKGLGKIKGRAFKVRKV"
     gene            192..1391
                     /locus_tag="VFX11_09485"
     CDS             192..1391
                     /locus_tag="VFX11_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4938895.1"
                     /translation="MQTATLTSGAERKLVLLLAAMQFAHIMDFMVMMPLGPQLMRVLD
                     ITTDQFGWLVSAYTVTAGVAGFVSVFLVDRFDRRHALTTLFAGFLLATLACGLAKDFH
                     ALLAARCMAGMFGGVLTAIIMALIADCFPESRRGAVTGKVMSGFAFAAVAGVPLGIYL
                     ANHFDWRMPFFFIAAFSFPMLLVLPRWLPSVPARDEGPIRIAGTLREVTGDRNHLYAF
                     VLVSALMLAGFSVIAYISPYMVKNVGLSETQLPLIYLYGGLGSLLTAPLVGKLSDHWG
                     RIRTFRVACLFSVIPLLAMTHLPVAPLLVVLIVTTFFMILVGGRVIAAIALFGSISHP
                     HLRGRFLSFNSAVQQVASGVAAFWPTLVLTQDAQGRLMHYDWVGYGAVVMTFFAIWLA
                     GRLEVRG"
     gene            complement(1392..4862)
                     /gene="mfd"
                     /locus_tag="VFX11_09490"
     CDS             complement(1392..4862)
                     /gene="mfd"
                     /locus_tag="VFX11_09490"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX4938896.1"
                     /translation="MTPPSPVAVSLPELPAKAGDRRHWGALGGAALALALAEAARRHP
                     GVLLAITADTQSATRLEDELGYFLGDELPVLHFSDWETLPYDQFSPHQDIISARLRTL
                     ATLPDLQRGILVVPVSTLAQRIAPRGWLGGRSLSLAVKQKLDGTEFRRQLEAAGYTAV
                     DTVFEHGEFAVRGSLIDLFPMGTEYPLRIELFDDEIDTLRRFDVDTQRTVENIDRVEL
                     LPAKEFPLDKSAIRRFRDAWAEAFEGDPKRCPVYQDVINGLSAGGIEYYLPLFFEETG
                     TLFDYLPANTLCMVDAGAHAQIGHVWSEIRNRYESRRHDTTRPLLPPERLFLRDNELF
                     EAINRFPRIEWQTGPVEERTGTVNLPFQTSPTLPVDGRSDSPLQSLLNFLQQQTNARI
                     LFTAESAGRRESLLELFARSDLKPSSVDNWQGFLQMTTPGTAITVAPLEQGLWLQDPE
                     LIVIAESQLFGQRVMQRRRRKASSQDLSGEMIVKNLSELNPGAPVVHIDHGVGRYQGL
                     VTLDIDNQPQEFLLLEYADKAKLYVPVSSLHLIARYSGADDAMAPLHRLGTEQWTKAK
                     RKAAEQVRDTAAELLNIYARRAARPGHAFDFSDIEYQHFAAGFPFEETADQAAAIIST
                     ITDLRAPRPMDRLVCGDVGFGKTEVAMRAAFVAVQGGRQVAVLVPTTLLAQQHYENFR
                     DRFADWPVKIEVLSRFKSVKEQNAALKQLEEGKVDIIIGTHKLLQEDVKFRNLGLIIV
                     DEEHRFGVRHKEALKNLRAEVDMLTLTATPIPRTLNMAFSGMRDLSIIATPPAKRLAV
                     KTFVRETSPEVVKEAMLRELLRGGQVYYLHNEVDSIEKCAQELQALVPEARIGIAHGQ
                     MRERELEQVMQQFYHKQFNVLVCTTIIETGIDVPSANTIIMDRADKLGLAQLHQLRGR
                     VGRSHHQAYAYLLTPNKKAMTGDAEKRLEAIAQADDLGAGFALASHDLEIRGAGELLG
                     EEQSGNINTVGFTLYMEMLDRAVKAIRSGKTPNLDQPLELGTEINLRISALIPDEYLP
                     DVHNRLMLYKRISNAKSPDDLNELQVEMIDRFGLLPDPTKNLFAVTALKLEAERLGIR
                     KIDAGAEGGRIEFSSETKIDPMRLIRLIQTQSRQYKLEGGDRLKFMLKSENAEERLQV
                     VHNVLDALQK"
     gene            complement(4859..>5059)
                     /locus_tag="VFX11_09495"
     CDS             complement(4859..>5059)
                     /locus_tag="VFX11_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938897.1"
                     /translation="FEAYDKKASLLSSVATMAWQLRLGPGMSRKANLFRGFCTGGTGT
                     PYPLQSARPFAGAFVVNPDLMP"
BASE COUNT          881 a         1427 c         1611 g         1140 t
ORIGIN      
        1 gacaagctgc ggcccggtga tatcctgggt gcgttgacgg gcgatgccgg cattcccggg
       61 gccatggtgg gcaagatcga tattttcgat atccaggcgt atgtcgccgt ggaccgtgat
      121 gtggccagtc aggccatgaa agggctgggc aaaatcaagg gccgtgcctt caaggtgagg
      181 aaagtctagc catgcagacg gcgacactga cctctggcgc cgaacgcaag ctggtcttgc
      241 tgctggcggc gatgcagttc gcccacatta tggatttcat ggtgatgatg ccgttgggcc
      301 cgcagctgat gcgtgtgctc gacatcacca cggaccagtt cggctggctg gtgtcggcct
      361 atacggtgac agcgggtgtt gccggctttg tcagtgtgtt tctcgttgac cgatttgatc
      421 ggcgccatgc gctgaccacc ctgtttgcgg gatttctgct ggctacgctg gcctgtggtc
      481 tggcaaagga tttccatgcg ttgcttgctg cccgctgcat ggccggcatg ttcggtggtg
      541 tactcacggc catcatcatg gcgctgattg cggactgttt tccggagtcg cgacgaggag
      601 cggtgaccgg caaggtcatg agtggttttg cgtttgctgc agtggcaggt gtgccgctgg
      661 gcatctacct tgccaaccac tttgactggc gcatgccgtt cttcttcatc gccgccttca
      721 gctttcccat gctgctggtg ctgccgcgtt ggctgccctc ggtgccagct cgcgacgagg
      781 gaccgatccg catcgcgggt acgttgcgag aggtaacggg tgaccgcaat cacctctatg
      841 cctttgtgct ggtgtcggcg ctgatgctgg ccggttttag cgtgattgcc tacataagtc
      901 cctatatggt gaagaatgtt gggctgtcgg agacgcagct cccgctcatc tatctctatg
      961 gcggactggg cagcctgctg acggccccgc tggtgggaaa gcttagtgat cactgggggc
     1021 ggataaggac ttttcgggtg gcctgcctgt tcagcgtgat tcccctgctg gcgatgaccc
     1081 atctgccggt agctccgctg ctggtggtgc tgatcgttac aaccttcttc atgatccttg
     1141 ttggcggacg tgtgattgcg gcgattgcct tgttcggctc gatcagccat ccgcatctgc
     1201 gcgggcgctt cctgagcttc aactcagccg tgcagcaggt ggcttcgggt gtggcagcct
     1261 tctggccgac gcttgtactc acgcaggatg ctcaaggccg cctgatgcat tacgactggg
     1321 tcggttatgg cgctgttgtc atgacctttt tcgccatctg gctggctggt cggctggaag
     1381 tacgcggctg attatttctg cagcgcatcc agcacgttgt gcaccacctg caagcgttcc
     1441 tctgcattct cgctcttcag catgaacttc aggcggtcgc cgccttccag cttgtactgg
     1501 cgggactgtg tctggatcag ccggatcagg cgcatcgggt cgatttttgt ttccgacgag
     1561 aactcgatgc ggccgccctc ggcaccagca tcgatcttgc ggatacccaa gcgctcggcc
     1621 tccagcttga gtgccgtgac cgcaaacagg tttttagtcg ggtccggcag cagtccgaag
     1681 cggtcgatca tttccacttg cagttcgttc aggtcgtctg gcgactttgc attgctgata
     1741 cgtttgtaga gcatcaggcg gttgtgcaca tccggcaggt attcgtccgg aatgagcgcg
     1801 ctgatgcgca ggttgatttc cgtgcccaat tccagcggct ggtccagatt gggcgtcttg
     1861 cccgaacgaa tggctttcac cgcgcgatcc agcatctcca tgtagagcgt gaagccgacg
     1921 gtgttgatgt tgccgctttg ctcctcgccg agcagttcgc cggcccctcg gatttccagg
     1981 tcgtggctgg ccagcgcaaa accagcgccc agatcgtccg cctgtgcgat ggcttccagt
     2041 cgcttttcag cgtcaccggt catggccttc ttgttgggtg tcagcaggta ggcataggcc
     2101 tggtgatgtg agcgtcccac acgaccacgc aactggtgca attgcgccaa ccccagtttg
     2161 tcggcgcgat ccatgatgat ggtgttggcg ctggggacgt cgatgccggt ttcgatgatg
     2221 gtggtgcata ccagcacgtt gaactgcttg tgataaaact gctgcatcac ctgctccaac
     2281 tcgcgttcgc gcatctggcc gtgggcaatg ccgatacggg cctccggtac aagcgcctgc
     2341 aactcctgtg cgcacttctc gatgctgtca acttcgttgt gcaggtagta cacctggccg
     2401 ccacgcagca actcgcgcag catggcttcc tttacaactt cagggctggt ttcacgaacg
     2461 aaggttttga ccgccaggcg ctttgcgggt ggcgtggcaa tgatggacag gtcgcgcatg
     2521 ccggagaacg ccatgttgag cgtgcgtgga atgggtgtag ctgtcagcgt gagcatgtcc
     2581 acttcggcgc gcagattctt cagggcttcc ttgtggcgca caccgaaccg gtgttcttcg
     2641 tcaacgatga taaggccgag gttcctgaac ttcacatctt cctgcagcag cttgtgcgtg
     2701 ccgatgatga tgtcgacctt gccctcttcc agctgtttca gcgcggcgtt ttgctccttg
     2761 acggacttga agcgcgacag tacctcgatc ttcaccggcc agtcggcaaa gcggtcacgg
     2821 aaattctcgt aatgctgttg cgccagcaag gtggtcggga ccagcacggc cacctggcgc
     2881 ccgccttgta cggccacgaa agcggcgcgc atggctactt cggttttgcc gaagcccaca
     2941 tcaccgcata ccagacggtc catcggacgg ggggcacgca ggtcggtaat ggtggagatg
     3001 atggcggcgg cctggtcggc tgtttcctca aaggggaatc cggccgcaaa gtgctggtac
     3061 tcaatatcgc tgaagtcaaa ggcatggcca ggacgcgcgg cgcggcgggc atagatgttg
     3121 agcagttcgg cggcggtatc gcggacctgt tcggcggcct tgcgcttggc cttggtccac
     3181 tgttcggtgc ccaggcgatg caacggtgcc attgcgtcgt cggcgccaga atagcgggca
     3241 atcaggtgca ggctggagac cggtacataa agcttggctt tgtcagcgta ctccagcagc
     3301 aggaattcct ggggctggtt gtcgatgtcc agcgtgacca gtccctgata gcgccccacg
     3361 ccgtggtcga tatgcacgac gggggcgcca gggttcagct cgctcaggtt cttgacgatc
     3421 atctcgccgg agaggtcctg gctgctggcc ttgcgccggc gacgttgcat cacgcgctgc
     3481 ccgaacagct gcgattcggc aatgacgatc agttccgggt cctgtaacca caagccctgt
     3541 tccagtggtg caacggtgat ggccgtacct ggtgtggtca tctgcaagaa gccctgccag
     3601 ttatcgacac tggaaggctt caggtctgag cgtgcaaaca gttccagcag gctttcacgg
     3661 cgacctgcac tttcggcggt aaacagaatg cgggcattgg tctgttgctg caggaaattc
     3721 agcagggatt gcaggggtga atcgctacga ccatccacgg gtaacgtggg cgaggtctgg
     3781 aaaggcaggt tgacagtgcc agtcctttct tcgacggggc ccgtttgcca ttcgatgcgt
     3841 ggaaagcggt tgatggcctc aaacagttcg ttgtcgcgca ggaaaagtct ttccggtggc
     3901 agcaatggac gcgtggtgtc gtggcggcgt gattcgtagc ggttgcggat ttcgctccac
     3961 acatggccga tctgtgcgtg cgcaccggca tccaccatgc aaagcgtatt cgcgggaaga
     4021 tagtcgaaca gcgtgccggt ttcttcaaaa aagagtggca ggtaatactc gatgccacca
     4081 gccgacaggc cgttgatcac atcctgataa accgggcaac gtttggggtc gccttcaaat
     4141 gcctcggccc atgcatcacg gaagcgacgg atggccgact tgtcgagcgg gaattccttg
     4201 gcaggcagca attcgacgcg gtcgatgttt tccaccgtgc gctgcgtgtc cacgtcaaag
     4261 cggcgcaggg tgtcgatctc atcgtcgaac agttcgatgc gcagcggata ttccgtgccc
     4321 atggggaaca ggtcgatcag gctgccgcgc acggcgaatt caccgtgctc gaaaacggtg
     4381 tcgacagcgg tatagccggc ggcctccagc tggcgtcgga actcggtgcc atccagtttc
     4441 tgttttacgg ccagcgacag cgagcgaccg cccagccagc ctcgcggggc gatgcgttgg
     4501 gccagtgtac tgaccggtac caccagaatg ccgcgctgca ggtcgggcag ggtggccagt
     4561 gtgcgcaggc gggcggaaat gatgtcctgg tgcggcgaga actggtcgta gggcagggtt
     4621 tcccagtcgc tgaaatgcag tacgggtagt tcgtcaccca gaaagtagcc tagttcgtcc
     4681 tctagccgcg tggctgactg ggtatcggct gtaatcgcca gcagcacccc tgggtggcgg
     4741 cgtgccgcct ctgccagcgc cagcgccagc gcagctccac ccaaggcgcc ccagtgccgg
     4801 cgatcacctg ctttggcagg gagttcgggg agactgacgg caactgggga tggcggggtc
     4861 atggcatcaa atccggatta acgacaaaag ccccagcgaa ggggcgcgcc gattgtagcg
     4921 gatatggtgt gcctgtaccg cctgtacaaa agcccctgaa aagattggcc ttgcggctca
     4981 ttccgggacc aagtctgagc tgccatgcca ttgtggcgac ggacgacaac agggaggctt
     5041 tcttgtcata ggcctcaaa
//
LOCUS       DASUEG010000507         7000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_433889, whole genome shotgun sequence.
ACCESSION   DASUEG010000507 DASUEG010000000
VERSION     DASUEG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433889"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /gene="dauA"
                     /locus_tag="VFX11_09500"
     CDS             complement(<1..1531)
                     /gene="dauA"
                     /locus_tag="VFX11_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEX4938898.1"
                     /translation="MPRKHLFSLRIANALRESCLSEPYTGARFSRDLLAGITVGIVAI
                     PLSMALAIATGVAPQYGLYTAIVAGFVIALCGGSRFSISGPTAAFIVILHPVALKFGL
                     AGLMVATLLSGLILVAMAFLRLGRYIEYIPESVTLGFTSGIAIVIVSLQIKDFFGLEI
                     AHMPESFLAKLQVLGSAMPDLRWADALVGVTTLLVLILWPRLKSRFTGHLPALVLGVG
                     VSLLLAQMGHDVATIGSRFSYLLPDGSAGRGIPPILPDLVWPWLQAGPQGGALEWNFD
                     TARDLFSAALAIAMLGAIESLLCAVVLDGMTGKRHSANSELLGQGIGNLVTPFFGGFT
                     ATAALARSATNVKAGAESPVAAMIHALVVLLGLVLLAPWLAYLPMASMAALLLMVAWN
                     MSEAHKVVALLRKAPLGDVLVLLICLSLTVLFDMVIAIGVGIVLAALLFMRDIAAMIK
                     VVDVTEHRKQFPHALPPGWRAVKISGPLFFAAADRVFGEISQLVVERGGLIVVMEAVP
                     LL"
     gene            1729..2193
                     /locus_tag="VFX11_09505"
     CDS             1729..2193
                     /locus_tag="VFX11_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006030754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX4938899.1"
                     /translation="MARTLNEWFSLYGESHQNKTNKQIHFVCVPAIYLSILGVLLWLP
                     TPGFLPSFINWATLSLLITLPFYWKLAPSIMVGMALFSGLCFALLGAFESAGLSVLWI
                     SIAIFAVAWVFQFIGHAVEGKKPSFFQDLQFLLVGPAWILGFLYRKAGIALS"
     gene            complement(2306..3085)
                     /locus_tag="VFX11_09510"
     CDS             complement(2306..3085)
                     /locus_tag="VFX11_09510"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX4938900.1"
                     /translation="MSNLIRERVTSVHHWNDTLFSFTTTRDAGLRFKNGHFIMIGLED
                     PEKGRPLMRAYSIASANYEEHLEFFSIKVENGPLTSKLQHVKVGDEIVVSTKPTGTLV
                     LDHLIPGRNLYLLSTGTGLAPFMSIIKDPETYAQYDKVILTHGVRHVSELAYTSLITE
                     ELPNNEFFGDQVREKLIYYPTVTREPFRNQGRLTDLMESGKLFSDIGLPAFDLEQDRF
                     MLCGSPSMLKDLTAILDARGFHEARHGDQGHYVIERAFVEK"
     gene            3196..4077
                     /locus_tag="VFX11_09515"
     CDS             3196..4077
                     /locus_tag="VFX11_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4938901.1"
                     /translation="MHYTLRQLQVFLATAHHENISRAAQSLSMSQSAASSALAELENQ
                     YDIQLFDRIGKRLQLNALGHALRPQAEALLQQAQDLDLALRQHAATGQIKIGATLTIG
                     NYLAVNIMAQFMRENPLSKVELQVANTATIAARIHNFELDLGLIEGELHDSELEVTPW
                     RDDELVVFCSPRHPLAKKRSLRDEDLLGATWILRETGSGTRQTFDRALHGLLPQLNVL
                     LELQHTEAIKRAVEADLGIGCLSKVALEDAFRRGTFVPLRVPHRDFSRFFYFILHKQK
                     YRSAGIERWLTLCKESM"
     gene            complement(4090..4257)
                     /locus_tag="VFX11_09520"
     CDS             complement(4090..4257)
                     /locus_tag="VFX11_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938902.1"
                     /translation="MMKSLKFWQNWFHRAETVPAVPAPVSGPEIERKPLSHIAVRAAL
                     KQKQRQARNAG"
     gene            complement(4502..4945)
                     /locus_tag="VFX11_09525"
     CDS             complement(4502..4945)
                     /locus_tag="VFX11_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4938903.1"
                     /translation="MRRTLFACTMFFWGIQSATSASGELAIIVHASNPSPEISLEQVA
                     SIFLGKSRRLPDGTKVIPIDQLEGEKPRSEFYDKVVQKSQSQLNSYWSRQIFTGKGQP
                     PFAVTGDSEVLEFIASNPNMIGYVDLATVEKAANAGDIKVLLTVR"
     gene            complement(5114..6664)
                     /locus_tag="VFX11_09530"
     CDS             complement(5114..6664)
                     /locus_tag="VFX11_09530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4938904.1"
                     /translation="MSLPMEPRPASRRCYRLAGWWMLSLWLPFSLAWGDQIEILYEPA
                     TAAQDENSQWLWLLAGLFLFAVLVATLIFMRIHHSLQLKDAMLRQTEARLREAQLIAR
                     IGSWSRDFGTGETYWSEEACRVLELEGREQEYRHYERLIHPDDSERVTEVVAAAYHQG
                     GNYQCDHRVICPGGKEKYIRLAGQVYLDDGNAPVRETGTVQDISERRAADLALQRSEA
                     RLRSILDATPYPIVILENAYGFPVLYVNRSTFQLFQLPFTTLPDEIKSQELWMDGQER
                     DAFFARVMQEKKVSDIELQLRKSDGQPFWAQLTGRLMDFAGTQAVFIAVVDVTERYHA
                     RLELERLATSDTLTGLLNRRSFLESANREWKRALRYRLPLTLLMLDIDHFKRVNDSYG
                     HGFGDDVIRRFGEVTRNCLREEDVLGRVAGEELCAVLTSAEEEGGYLVAERIRKRWQE
                     EVFEFAGARFNFTVSIGVARMLTDHETVDTVMERAEVGLRRAKRAGRNCVIVNSGEID
                     DGRRQTPA"
BASE COUNT         1473 a         2182 c         1974 g         1371 t
ORIGIN      
        1 ccagcagcgg caccgcctcc atcaccacga tcaacccgcc gcgctcaacc accagctggg
       61 aaatctcgcc aaacacccgg tccgccgccg cgaagaacaa cggaccactg attttcaccg
      121 cgcgccagcc cggcggcaga gcatggggaa actgcttgcg atgttccgtg acatccacca
      181 ccttgatcat ggcggcaata tcgcgcatga acagcagcgc tgccagcaca atgcccacgc
      241 caatcgcgat caccatgtcg aacagcaccg tcagcgacag gcagatcagc agcaccagca
      301 catcccccaa cggcgccttg cgcagcaacg ccaccacctt gtgtgcctcg ctcatgttcc
      361 aggccaccat cagcagcaac gccgccatcg atgccatcgg caggtacgcc agccacggcg
      421 ccagcagcac cagccccaac aacaccacca gcgcatggat catggccgcc accggcgatt
      481 ccgcaccggc tttcacattg gtagcagaac gggccagtgc tgctgtcgcg gtaaaaccac
      541 cgaaaaatgg cgtcaccaga ttgccgatac cctgccccag caattcgctg ttggcgctgt
      601 gacgcttgcc ggtcatccca tccagcacca ccgcgcacag caaagattca atcgcaccca
      661 gcatcgcaat cgccagcgcc gcggaaaaca gatcgcgcgc cgtatcgaaa ttccattcca
      721 gcgcgccgcc ctggggtccg gcctgcaacc agggccagac cagatccggc agaatcggcg
      781 gaatgccacg cccggcggaa ccatccggca gcagataact gaagcggctg ccgatagtcg
      841 ccacatcgtg ccccatctgc gccagcagca acgacacgcc gacacccagc accagtgccg
      901 gcaaatggcc ggtgaaacgg gatttcagcc gtggccacag gatcaacacc agcagcgttg
      961 tcacccccac cagcgcatcg gcccagcgca ggtccggcat ggccgatccc agcacctgca
     1021 acttggccag gaaggattcc ggcatgtggg cgatctccag cccgaaaaaa tccttgatct
     1081 gcagcgatac aatgacgatg gcaatgcccg aggtgaatcc cagcgtcacc gattccggaa
     1141 tatattcgat gtagcggccc agcctcagga acgccatcgc caccagaatc agaccggaca
     1201 acagcgtcgc caccatcagc cccgccaggc cgaatttcag cgccaccgga tgcagaatca
     1261 cgatgaaggc agctgtgggt ccggaaatgc tgaagcggga gccaccacac aacgcaatca
     1321 caaaacccgc gacgattgcc gtgtacaggc cgtattgcgg cgccactccc gtggcaattg
     1381 ccagcgccat cgacaacgga atagccacga tgccgaccgt gatgcccgcc agcagatcgc
     1441 ggctgaagcg ggccccggtg tagggttcgc tcaggcagga ttcgcgcagg gcattggcaa
     1501 tacggagtga gaagagatgc ttgcggggca tgaagcggcc tctggatacg gcaggaaacg
     1561 actgtcatcg aagcatggaa catggcgcca gtgcggtcaa cgggattgtc cgcaccagtc
     1621 aatgcacaat cgccaccagc gccgctatga tgctgtcttc gaagcgcgtt gcgttggcgc
     1681 tttccgtccg ttgcacacga ttttcatttc cgacaccgga gcatcgccat ggccagaacc
     1741 ctgaacgaat ggttttccct gtacggtgaa agccaccaga acaaaaccaa caaacagatt
     1801 catttcgtct gcgtgcccgc catctacctc agcattctgg gcgtgctctt gtggctgcca
     1861 acgccaggct tcctgccctc tttcatcaac tgggccacgc tgtcactact gatcacactg
     1921 ccgttttact ggaagctggc accctcgatc atggtgggca tggcgttgtt ttccgggctg
     1981 tgctttgcat tgctgggtgc ctttgaaagt gcggggcttt ccgtgctgtg gatatccatc
     2041 gccatcttcg cagtggcctg ggttttccag ttcatcggcc acgcggtgga aggcaagaag
     2101 ccgtccttct tccaggatct gcaattcctg ctggtgggcc cggcctggat tctgggcttc
     2161 ctgtaccgta aggcgggcat tgcgttgtct tgagtactga cgattgatgg gcacaaacga
     2221 aaacgccggt gctggaatca gccaccggcg ttttttatgg gatcttgatt agctatcgta
     2281 tttggctaca ggcagcgtcg tccgcttact tctccacgaa tgcccgctcg atcacatagt
     2341 gaccctggtc gccgtgacgg gcttcatgaa atccgcgtgc atccagaatc gccgtcagat
     2401 ccttcaacat gctggggcta ccacacaaca tgaagcggtc ctgttccaga tcgaacgccg
     2461 gcagaccgat gtcgctgaac agcttgccgc tttccatcag gtcagtcagg cgaccctggt
     2521 tgcggaacgg ttcacgggtc accgtggggt agtagatcag cttttcacgt acctggtcgc
     2581 cgaaaaattc attgttgggc agttcttccg tgatcaggct ggtataggcc agttccgaca
     2641 catgacgcac gccgtgggtc agaatcacct tgtcatactg ggcgtaggtt tccggatctt
     2701 tgatgatgct catgaaaggc gccagaccgg tgccggtgct caacagatac agattgcggc
     2761 cggggatcag gtgatccagc accagggtgc ctgtgggttt ggtgctcaca acgatctcgt
     2821 cccccacctt cacgtgttgc agcttggatg tcagcgggcc gttttccacc ttgatgctga
     2881 aaaactccag gtgttcctca tagttggcgc tggcgatgct gtaggcacgc atcagcgggc
     2941 ggcccttctc cgggtcctcc aggccgatca tgatgaaatg gccgttcttg aagcgcaaac
     3001 cggcatcgcg ggtggtggta aagctgaaca gggtgtcgtt ccagtggtgg acgctggtga
     3061 cgcgctcgcg aatcaggttg gacatgacag cactccttga agcggaaaac aggccttgga
     3121 ttgcgcgcag tatgccggca gactatccta tcggtaaaat gaataattta aataaccttt
     3181 atcgatattt cggatatgca ctacaccctg cgccaacttc aggttttcct cgccaccgcg
     3241 caccacgaga acatctcacg ggcggcccag agcctatcca tgtcccagtc tgccgccagc
     3301 agcgccctgg cggaactgga aaaccagtac gacatccagc tgtttgaccg cattggcaaa
     3361 cgtctgcaac tgaacgccct cgggcacgcc ctgcggccgc aagccgaggc cctgctgcaa
     3421 caggcgcagg atctggatct ggccctgcgc cagcatgccg ccaccggtca gatcaagatc
     3481 ggcgccaccc tcaccatcgg taattacctg gccgtgaaca tcatggcgca gttcatgcgg
     3541 gaaaaccctc tgtcgaaagt ggagctgcag gtagcgaaca ccgcgacgat tgccgcccgc
     3601 atccacaatt tcgagctgga tctcgggctg atcgaggggg aactgcacga cagcgagctg
     3661 gaagtgacgc cctggcgcga cgatgagctg gtggtgttct gctcgccacg gcatccgctg
     3721 gcgaaaaagc gcagcctgag ggatgaggac ctgctggggg ccacctggat tctgcgggaa
     3781 accggatcag gcacccggca gaccttcgac cgcgccctgc acggcctgct gccgcaactg
     3841 aacgtgctgc tggaactgca gcataccgag gcgatcaagc gggcggtgga ggcggacctg
     3901 gggatcggtt gcctgtcaaa agtggcgctg gaggatgctt tccggcgcgg taccttcgta
     3961 ccgttgcggg taccgcaccg ggatttcagc cgcttcttct atttcatcct gcacaagcag
     4021 aagtaccgca gtgcaggcat cgagcgctgg ctgacgttgt gcaaggagag tatgtgagcg
     4081 caaccccggt cacccggcat tgcgcgcctg tcgctgtttc tgcttcaacg cggcacgcac
     4141 tgcgatatgc gaaagaggct tgcgttcgat ttccgggcca gacaccggtg ccggcaccgc
     4201 cggaaccgtt tccgcgcgat ggaaccagtt ctgccaaaac ttcagagact tcatcatggc
     4261 gactacctcc cgtacctgcg actgtgctcg caacaaaaaa ccccggtcgg ttgccctccg
     4321 gggttttggg gattccggaa ggcgacgctg gcaccgcctt cccgcgttga gtgcgtcagg
     4381 aaagtggcgt ctggggcaaa acctgtttca atttgtcggt attgcgtctg gctggctgca
     4441 tgagattcga atgttgtgta cgtgtgttaa atatagacca agaacaaagc gcgcgccgga
     4501 atcagcgcac agtcagcaaa actttaatgt cgccggcatt ggcggctttc tccactgtcg
     4561 ccagatccac atagccaatc atgttgggat tggacgcgat gaattccagc acttcggaat
     4621 ccccggtcac cgcgaacggc ggctggccct tgcctgtgaa aatctggcgc gaccaatagg
     4681 aattcagttg ggactggctc ttctgcacca ccttgtcgta aaactcggag cgcggctttt
     4741 ccccttccag ctggtcgatg ggaatcacct tggtaccgtc cggcaggcgg cgggatttgc
     4801 cgaggaaaat gctggcgacc tgctccagcg agatttccgg cgacgggttg ctggcgtgga
     4861 cgataatcgc cagttcgccg ctggcagacg tcgctgactg gataccccag aaaaacatgg
     4921 tgcaagcaaa tagtgttctg cgcattttca tcctttggtc atcctctcgc agtgtgagcc
     4981 tgggacgaat tccacattcc agcgtttctc gtgaaaacac aggcccaaat tttcgtccat
     5041 ccggttgggt caggtcggct cggtcagata aataaagtcg ataaacgtct aatcttcaag
     5101 tttttcaact gcttcaggcc ggtgtctggc gacggccgtc gtcaatctcg ccactgttca
     5161 cgatcacgca gttgcgtccg gcccgcttgg cccggcgcag gccgacctcg gcacgctcca
     5221 tcaccgtgtc cactgtttcg tgatccgtca gcatccgcgc cacgccaatg ctgaccgtga
     5281 aattgaagcg ggcaccggca aattcgaaca cttcttcctg ccagcgcttg cgaatgcgct
     5341 ccgccaccag gtagcctccc tcctcctccg ccgaggtcag taccgcgcat aactcctcgc
     5401 cggcgacgcg gccaagcaca tcctcttccc gcaggcagtt gcgggtcact tcgccaaagc
     5461 ggcggatgac gtcgtcgcca aaaccgtggc catagctgtc gttcacccgc ttgaaatgat
     5521 caatgtccag catcagcagg gtcagaggca accgataacg caaggcacgt ttccactcgc
     5581 gattggcgga ttccaggaat gatcgccggt tgagcagtcc ggtcagcgta tcgctggtgg
     5641 ccagccgctc gagttccaga cgggcatgat aacgctcggt aacatccaca acggcgatga
     5701 acactgcctg ggtgccggcg aaatccatca ggcgaccggt cagctgcgcc cagaacggct
     5761 ggccatcgga tttgcgcagt tgcaattcga tgtcgctgac tttcttttcc tgcatcacgc
     5821 gggcaaaaaa tgcgtcgcgc tcctggccgt ccatccacag ttcctgggac ttgatttcat
     5881 ccggcaaggt tgtaaagggt agctggaata actggaacgt gctgcgattg acgtacagca
     5941 cggggaaacc gtaagcgttc tccaggatca cgatgggata cggcgtggcg tcgagaatgg
     6001 agcgcaggcg cgcctcggag cgctgcagcg ccaggtcggc agcacggcgt tcggaaatat
     6061 cctgcaccgt gccggtttcc cgcaccggcg cattgccgtc gtccagatac acctggcctg
     6121 ccagccggat atatttttcc ttgccaccgg ggcaaatgac gcggtggtcg cactggtaat
     6181 tgccgccctg gtgatacgct gctgccacta cttcggtaac gcgctcgctg tcgtccgggt
     6241 gaatcagccg ttcgtagtgc cggtattcct gctcgcgccc ttccagctcc agcacgcgac
     6301 aggcttcctc ggaccagtag gtttcgccgg tgccgaaatc gcggctccag ctgccaatgc
     6361 gggcaatcag ctgagcctcc ctgagtcggg cctcggtttg ccgcagcatc gcgtccttca
     6421 attgcagtga atggtggatg cgcataaaaa tcagggtggc aaccaagacg gcaaacagga
     6481 acagtcctgc cagcagccac agccactggc tgttttcatc ctgagcggcc gtggcgggtt
     6541 cgtaaaggat ttcgatctgg tcaccccacg ccagcgagaa cggcaaccac aacgacagca
     6601 tccaccaccc ggcaaggcgg taacagcggc gggaagcagg gcgcggttcc atcggcaacg
     6661 acaaggggca ctccaggcac aggtaatcgg tccaactcag tatagtaaag cccgccacac
     6721 cagccgtgtc gcgtggcggt ggcgccgctt tttattttcc cggggattga tttagccaaa
     6781 tccggcaact agagttccgc tgcgcccatt ggcggcccct tttcaaactg cagatactcg
     6841 aaatgatgga ttcacacgcc catatcggtt ttcagattcc ggaaaccctc taccaggatg
     6901 taaccgggct gatcgaacag atccgcgcac gcccacagca gcgccagcac ggcaaggccg
     6961 ctgccaacgt tgtatctgcc ttgctgcacc agggcatcca
//
LOCUS       DASUEG010000508         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_383475, whole genome shotgun sequence.
ACCESSION   DASUEG010000508 DASUEG010000000
VERSION     DASUEG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383475"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..568)
                     /locus_tag="VFX11_09535"
     CDS             complement(29..568)
                     /locus_tag="VFX11_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4938905.1"
                     /translation="MDLSILAAARDGDRSAMEALLLQSQPDIRRYAMRHCVISDIDDA
                     VQEVLIIVAKHIESLKFLAAFSSWLFKTVQRECRRLGRVTLNYDPFEEEHLDSWLQAH
                     SDDELINELISAIEKLPQEHREVILLKDIQQFSNREIASELGITVAAVKSRVHRAREL
                     TRAMLLDEPVPGIADVGSD"
     gene            753..1670
                     /locus_tag="VFX11_09540"
     CDS             753..1670
                     /locus_tag="VFX11_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4938906.1"
                     /translation="MQKTKILLVMLLTCFLWACKTSVQEEKGPRAPEFPTIPSSKTIV
                     FVHGLYLTPASWSEWETYFQDLGFTTYSPAWPYHDLSVDEQNALHPNSELGTLTLPMV
                     LQHYRDFIATLEEPPILIGHSMGGLVVQLLLEENIAAAGIVIHSAPPFGVISIEPKFL
                     KASWPLLNPALNPSEPSKLTFKQFQHGFVNGMDLETQQDAYAHYCVPDSLRVGRSSLS
                     RVTKVDKSVSRPPLLIIAGDRDNTVTASMNYVNFKLFHKTPAITDYKQFPDRNHWTLQ
                     QDGWQAVADYAAHWIEENSERTTGVSLAE"
     gene            1910..>2306
                     /locus_tag="VFX11_09545"
     CDS             1910..>2306
                     /locus_tag="VFX11_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4938907.1"
                     /translation="MSDLPETPGFDALGMPDFILRTLTELGYESPSPIQAAAIPVFLS
                     GSDLIGQAQTGTGKTAAFALPILAQLDDENTKPQALIMTPTRELAIQVAEAFQRYARY
                     RKNFRIAPIYGGQEFYPQLKLLRQGVQVVV"
BASE COUNT          558 a          685 c          548 g          515 t
ORIGIN      
        1 ccacacgcaa ggtgatgaag aacctgcttt aatccgaccc cacatcggca atacccggca
       61 cgggctcatc caggagcatg gcccgtgtca gctcccgcgc cctgtgaacg cggcttttga
      121 cagctgcgac cgtaatcccc agctcactcg caatctccct gttggaaaat tgctggatat
      181 ccttcagcag tatcacttca cgatgctcct gcggcagctt ctcaatagca ctgatgagct
      241 cgtttatcag ttcatcgtca gaatgcgcct gcagccagct gtccagatgc tcctcttcga
      301 aggggtcgta attcagcgtc actctcccca gcctgcggca ttctcgctgc accgttttga
      361 acagccagct ggaaaacgcc gccaggaact tcagcgattc aatgtgtttt gcgacgatga
      421 tcagcacttc ctgcacggca tcatctatgt cgctgataac acagtgtcgc atcgcatagc
      481 gccggatgtc cggctgactc tgcaacagca atgcttccat tgcagacctg tcgccgtcgc
      541 gtgcagctgc aagaattgaa agatccatgc tacccctccg tccaaaactg acttgcctgt
      601 acccaaaata tccggcggcg aaaaacagtg caaaaaaaca tgattcatcg agaatccttt
      661 tcctggtttc gttcctctta tggaaaagca agtttgaaaa ttgctgagaa cgcccgacgc
      721 aaattgaatc taccataagg aacttgaaag tcatgcaaaa aactaaaatc cttcttgtga
      781 tgctattgac ctgctttttg tgggcgtgca aaacaagcgt gcaggaagaa aaaggcccaa
      841 gggcgccaga atttccgacc atcccgtctt ctaaaactat cgtctttgtc cacggtttgt
      901 atttgacacc ggcatcctgg agtgagtggg aaacctattt tcaggatctg ggctttacga
      961 cctatagccc tgcctggcca tatcacgacc tgtctgtcga cgaacaaaat gcgctgcacc
     1021 ccaacagcga actgggcacg ttgacattgc ccatggtgct tcagcattac cgcgacttta
     1081 ttgcgacact ggaggagcca cccatactca ttggccattc aatgggcggc ctggtcgttc
     1141 agctactgct ggaggaaaat attgcggcgg caggaatcgt gatccattcc gcccctccct
     1201 tcggcgtgat atccattgaa ccgaaatttc tgaaagccag ctggccgctg ctcaatccgg
     1261 cattgaatcc ctctgaaccg tccaagctca cgttcaagca gtttcagcac ggttttgtaa
     1321 atggaatgga tctggaaacc cagcaggacg cttatgcaca ttactgtgtt ccggattctc
     1381 tcagagtcgg acgttcatca ctgagccgtg tgacgaaagt ggacaaatcc gtttcccgcc
     1441 caccgctgtt aatcatcgcg ggtgaccggg acaacaccgt cacggccagc atgaactacg
     1501 tgaacttcaa gctgtttcat aaaacgccag ccattacaga ctacaaacag ttcccggaca
     1561 gaaatcactg gacgttacaa caggatggat ggcaagccgt ggcggactat gctgcccact
     1621 ggatagaaga aaactcggaa cgcacaaccg gagtatcgct agccgaatag ccggtgcaca
     1681 cccggctcag tcttgcgtta tcgcgtctca actcgctgaa taaaataccg ctgacaacgc
     1741 gttgagacgc aaaccaaaaa cagcaagatg ttcctggcac gtccccttgg ttcttttatt
     1801 gcctattgac tttatataca gagtccactg caaacctgta caatgcacgc ccgctgaacg
     1861 actgctaccc ttactctcct gagtagctcc cgaccgaagg ccgcgccaaa tgtctgattt
     1921 acctgaaact cctgggtttg atgccctggg catgcccgat ttcatcctca gaaccctgac
     1981 ggaactgggc tatgaatccc cctcccccat tcaggcagcg gccattcccg tcttcctcag
     2041 cggcagcgac ctgatcggtc aggcccagac cggcacaggc aagaccgccg ccttcgccct
     2101 gccgatcctg gcgcagctgg acgatgaaaa caccaagccc caggcgctga tcatgacgcc
     2161 gacacgcgag ctggcaatcc aggtggccga ggctttccag cgctacgcgc gctaccgcaa
     2221 gaatttccgc attgcgccca tttacggcgg ccaggaattt tatccgcagc tgaaactgct
     2281 gcgacagggt gtacaggtgg tggtgg
//
LOCUS       DASUEG010000509         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_338381, whole genome shotgun sequence.
ACCESSION   DASUEG010000509 DASUEG010000000
VERSION     DASUEG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338381"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..472)
                     /locus_tag="VFX11_09550"
     CDS             complement(8..472)
                     /locus_tag="VFX11_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4938908.1"
                     /translation="MEIQNDCVVAIHYTLTNADGDVIDSSQGRDPLKYLHGYGNIIRG
                     LEQALAGKTVGEALNVVIEAVDAYGERDEDLVQEVPRAAFQGIDDIEVGMQFHAQTPD
                     GGAIPVKIIAVDDSIVTVDGNHELAGERLFFAVTIEDIRVASPEEIDHGHVH"
     gene            complement(681..2033)
                     /locus_tag="VFX11_09555"
     CDS             complement(681..2033)
                     /locus_tag="VFX11_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938909.1"
                     /translation="MSFSNHFFRLGLLGALGFLLSACDDSTVVTGGQCPPPPAAFSAA
                     PAPAMAGQSSVDVELGRTYLLNRVRTMLATDPTQDSGAFIGNISHRDVTDNAGKRRSQ
                     LELTLEPWLKGQNGQPASLQRYYKLTLQLTPRLVTPTTVPDVNVRRQLLQCDPATAVD
                     CDTRESAMLSFDLVELYNTSFSRPACTTPDMIDSRLVPQIYDLLARQAPLQLPTDAVG
                     ALLASAAGTPVQLTDVNVSLDNLLKLGLQYTIGTTHAFDTQTMLLGRFPNRDWLVDID
                     TSIMTTAVRGRLQTELNNSVTTMGSTVTSFSAQFLPGEIRATGSVAVPPRGVCGSTTT
                     LGINIVAPALACRDINGQSVIAARVDAQPTAPAFCLILNTFWDNLNVGIASDALPTFP
                     TLAAVQFPAGPNDVFYGTDLDLDNAFTIAGRSTVMDALDATATPPLRSPLPGKCPGVP
                     "
     gene            complement(2206..>2815)
                     /locus_tag="VFX11_09560"
     CDS             complement(2206..>2815)
                     /locus_tag="VFX11_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4938910.1"
                     /translation="VDAINAKSDVTGVTATVNDQGELELNAQDGRDIVVSTASIAVTN
                     RLFGGGENRFDAAFNDLRVSGEVTVSADDSIQFSGADQASAGLSALQQDNAEATGTVA
                     SIDVRSVAGAQEAVDSVDAALNQIDTYRASMGAIQNRFESSIRNLSAVSESQTAALSR
                     TRDTDYAAQIAEVSREQVRRQAGIALQAQANALSRQVLALLS"
BASE COUNT          533 a          820 c          931 g          531 t
ORIGIN      
        1 agtcggatca gtgcacgtgg ccgtggtcga tttcttccgg tgatgccacg cggatatctt
       61 cgatggtcac cgcaaagaac agacgctcgc ctgccagctc gtgattaccg tcgacggtta
      121 cgatggaatc gtccactgca atgattttca cgggaatggc gccgccatcc ggcgtctggg
      181 cgtgaaactg catgcccact tcgatatcgt cgatgccctg gaacgccgcc cgcggcactt
      241 cctgcaccag atcctcgtca cgttcaccgt aggcgtccac cgcctcgatc accacgttca
      301 gtgcctcgcc caccgtcttg cctgccagcg cctgctccag gccgcgaatg atgttgccgt
      361 agccgtgcag gtatttcagc ggatcacgac cctgggagga atcaatgacg tcgccgtcag
      421 cgttggtcag cgtgtagtgg atggcaacga cgcaatcgtt ctggatttcc attggtttac
      481 ctcatgctcg ccggtaagga gggaaggggg tcggcagccc gcaaagggta ccgccggacg
      541 gggtgtaagg acagaaaaaa gtggcgcggg gcggctgagt ggaccggcag gaagtgctgc
      601 ggcgctcccg gaaccggtcc gggagcgtct ggaaggctgt tttggggtgt tgcgttgact
      661 atgggatgaa agcgggctgc tcaaggcacc ccgggacatt tccccggcag cggcgaacgc
      721 aacggcggtg tggcggtggc atccagcgca tccattaccg tactgcggcc ggcaatggta
      781 aaggcgttgt ccaggtcgag atcggtacca tagaacacgt cgttggggcc ggcagggaac
      841 tgcacggcgg ccagggtggg aaaggtcggc agcgcatccg aggcaattcc cacgttcagg
      901 ttgtcccaga aggtgttgag gatcaggcaa aaggcgggtg cggtgggttg cgcgtcgact
      961 ctggccgcaa tcacagactg cccgttgata tcacgacagg ccagtgccgg cgccacaata
     1021 ttgatcccca gtgtcgtagt ggaaccgcaa acaccgcgcg gcggaaccgc tacgctgccg
     1081 gtggcgcgaa tctcgcccgg caaaaactgg gcgctgaacg aggtgacggt gctgcccatg
     1141 gtggtgacgc tgttgttcag ttcagtctgc aaccggccgc ggacggctgt tgtcatgatg
     1201 ctggtgtcga tatccaccag ccagtcgcga ttggggaagc ggcccagcag catggtctgg
     1261 gtatcgaacg catgggtagt ccctatggtg tactgcaatc ccagcttcag caggttgtcg
     1321 agtgaaacgt tcacatcggt caattgcacc ggtgttcccg ctgcgctggc cagcagggca
     1381 ccgacagcgt cggtgggcaa ctgcagcggt gcctgtcggg ccagcagatc atagatctgc
     1441 ggcaccagtc tggagtcgat catatccggt gtggtgcagg cgggccggct gaacgatgtg
     1501 ttgtacaact ccaccagatc gaacgacagc attgcgcttt cgcgcgtatc gcagtcgacg
     1561 gcggtggccg gatcgcattg cagcaattgc cggcgcacgt tcacgtcagg caccgtggtg
     1621 ggggtgacca ggcgcggcgt cagttgcagg gtcagcttgt aatagcgctg cagggatgcc
     1681 ggttgcccgt tctggccttt cagccagggt tccagcgtga gttcgagctg gctgcggcgt
     1741 ttgcctgcat tatccgttac gtcccggtga ctgatattgc cgataaaggc gccggaatcc
     1801 tgggtggggt cggtggccag catggtgcgc acccgattca gcagataggt gcggccaagt
     1861 tccacatcga cactggactg gcccgccatg gccggcgcgg gcgcagcgct gaatgccgcc
     1921 ggtggtggtg ggcactggcc gccggtgacc acagtggaat cgtcgcaggc cgacagcaga
     1981 aagcccagcg cacccaacag ccccaatcta aaaaaatggt tggaaaaaga catgagaacc
     2041 tcacgcaagc ggaaaaaaat aaaaggagga caaccaaatc acagacgact gccgggcggc
     2101 aactgtggac acaacgtagc attaattgag gggggcgggt aggtgggcag gccaactgcc
     2161 tggaccggca atgcattcag cgtcgtgatg cggcccgcaa cgttgtcagc tcagcaacgc
     2221 gagcacctgc cgcgataacg cattggcctg ggcctgcagt gcaatgccgg cctgacgcct
     2281 cacctgttcc cgcgagactt cggcaatctg cgcggcgtag tcggtgtcgc gggtgcgcga
     2341 cagggcggcg gtctgcgact ccgacaccgc cgacagattg cggatgctgg attcaaagcg
     2401 gttctggatg gcacccatgc tggcgcggta ggtgtcgatc tggttcaggg cggcatcgac
     2461 ggaatccacg gcttcctgcg cgcccgccac cgagcgcacg tcaatgctgg ccacagtgcc
     2521 ggtggcctcg gcattgtctt gctgcaacgc actgagcccg gcactggcct gatcggcgcc
     2581 gctgaactgg atgctgtcgt cggcgctgac ggtgacttcg ccgctcaccc gcaggtcatt
     2641 gaaggctgcg tcgaaacggt tttcgccgcc gccgaacaag cggttggtca ccgcaatgga
     2701 ggccgtggaa accacgatgt cgcggccgtc ctgcgcattc agttccagtt ccccctgatc
     2761 gtttacggtg gcggtcacgc cggtcacatc gcttttggca ttgatggcgt ccacc
//
LOCUS       DASUEG010000510         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2317, whole genome shotgun sequence.
ACCESSION   DASUEG010000510 DASUEG010000000
VERSION     DASUEG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1796
                     /gene="xrtD"
                     /locus_tag="VFX11_09565"
     CDS             222..1796
                     /gene="xrtD"
                     /locus_tag="VFX11_09565"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HEX4938911.1"
                     /translation="MTGVNVNWREPSLMWALLLLASVVLAIAFASEAGSLYLAWQTQE
                     EYSHGFLIPFIALFLIYQKKDQLEKIPFSGSWVGVAIVVFGALLHLAGRLAVVSTMGQ
                     YALVICITGLFLAFMGWRAFKLVWVAMFMLIFMVKLPTFFYNNLSSQLQLISSQIGVW
                     LIRLFDISVYLEGNVIDLGAMKLQVVEACSGLRYLFPLMALSLILAIFYKAPLWMRVL
                     VFLSSMPVTVLMNSFRIGAIGITVEYWGKQMAEGFLHDFEGWVVFMAAFVVLFLEIIV
                     LNKLRGEKRPLQEVLSLELPAPTDPRVPSRVRSVPLQFYVATAVLLGFLLVNQTLSVA
                     EAVYPVREKLNRFPGLVDGRLATPGTLDPVFLGELQLDDYMMADYKSAARTPINLYIA
                     YYDVQAEGAGTIHSPRSCLPGSGWRIKTIDQRLLSDIAVAGKPLEVSRALIAKDNQQV
                     LVYYWMQQRGRIITSELMAKWWIFWDRLTLGRSDGALVRVMMEVGPYQDIAVADQQMQ
                     QFVRAIVPDLPRFIPD"
     gene            2016..>3707
                     /locus_tag="VFX11_09570"
     CDS             2016..>3707
                     /locus_tag="VFX11_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX4938912.1"
                     /translation="MKQVLQDLRTGATYIDNVPCPMVQSGHVLIRTTQTLVSAGTEKM
                     LVDFGKASLVQKARQQPDKVRMVLDKIRTDGLLPTVEAVRAKLDQPIPMGYCNVGVVV
                     QCGANVEGFQPGDRVVSNGHHAEMVTVPKHLCARIPPSVSDDQASFTVMASIALQGIR
                     LAQPELGETFAVIGLGLVGLLTVQLLRANGCRVIGIDFDAGRVSMARQLGAESIVLRD
                     GADPVAFAQTFTGGVGVDGVLITASTRSNDPVHQAATMSRKRGRIVLVGVVGLELSRA
                     DFYEKELSFQVSCSYGPGRYDPDYEQKGIDYPIGYVRWTEQRNFEAVLAAMASGAVHP
                     EGMITSRFRLDEAAAAYAAILQGGALGVVLECDRQQPAAQVLASTIETNSAPAALTAT
                     IRVGCIGSGNYASRVLFPAFRKAGVSLATVACTGGTSGTHAAKKFGFLKITTDSDTVI
                     ADHQIDLVTVATRHDTHARYVNAALLAGKHVFVEKPLAITQGELISIEHAYTSTRGHI
                     NPPQLMIGFNRRFSALVTPVKQVLDTARDPACINMTINAGEIPPSSWIQDLQVGGG"
BASE COUNT          722 a          968 c         1139 g          878 t
ORIGIN      
        1 gcagcgggtt agcggccgta gccatgacct gaaacaccag tgagtgggga atgcttaaaa
       61 agaccaccaa cgctagcaat tgtccgtgac gcctgtatga cggtcgcgcc gggcttgccg
      121 ttaataaatg tttaaccttc cggtaatact atggcgcagg ttatgtcttt ttgtttcgta
      181 cattgttgtg gtggggagct gttttgggta attcgaatga gatgaccggg gtaaatgtca
      241 actggcgcga gcccagtctg atgtgggctc ttttgctgct ggcgagtgtg gttcttgcta
      301 tcgcttttgc atctgaagcg ggttctctct acctcgcctg gcaaacccag gaagaataca
      361 gccacggctt cctgattccc ttcatcgccc tgttcctgat ctaccagaaa aaggaccagc
      421 tcgaaaaaat cccgttttcc ggatcgtggg ttggcgttgc catcgtggtg ttcggtgcgt
      481 tgctccacct ggcgggccgt ctggcagtgg tctccaccat gggtcagtat gctctggtca
      541 tttgcattac gggcctgttc ctggcgttca tgggctggcg cgcgttcaaa ctggtctggg
      601 tggccatgtt catgctgatc ttcatggtca aactgcccac atttttctat aacaaccttt
      661 ccagccagct gcagctgatc tcgtcgcaga taggagtctg gctgatccgt ctgttcgata
      721 tcagtgttta cctggaaggc aatgtcatcg atctgggggc gatgaaattg caggtagtgg
      781 aagcctgcag cggattgcgc tatctgtttc cgttgatggc gctgagcctg atcctggcca
      841 tcttctacaa ggcgccgctg tggatgcggg tactggtttt tctgtccagc atgcccgtta
      901 cggttctgat gaacagcttt cgcatcggcg cgatcggcat cacggtggag tactggggca
      961 aacagatggc cgaaggattc ctgcacgatt tcgaaggctg ggttgtgttc atggccgcat
     1021 ttgtggtgct gtttctggaa atcatcgtgt tgaataagct gcgcggcgaa aaacgtccat
     1081 tgcaggaagt gctgtcactg gagctgccgg caccgacaga cccccgggtg ccgagcaggg
     1141 tacggtccgt tccgcttcag ttctacgttg ccacggcggt gttgctgggg ttcctgctgg
     1201 tcaatcaaac cctgtcggtg gcggaagcgg tttatccggt gcgcgaaaaa ctgaatcgct
     1261 ttccagggct ggtggatggc cggctggcaa cgccgggtac gctggaccct gtcttcctgg
     1321 gtgagttgca gctggatgat tacatgatgg cggattacaa atccgcggcg cgaacgccga
     1381 tcaacctgta tatcgcctat tacgatgtcc aggccgaggg tgctggcacg attcactcgc
     1441 cccggtcctg cctgccagga tcgggttggc gcatcaaaac gatcgatcag cggctattgt
     1501 cggatattgc ggttgcaggg aaaccgctgg aggtcagtcg ggccctgatc gccaaggata
     1561 accagcaggt actggtgtat tactggatgc agcagcgagg ccgcatcatt accagcgagc
     1621 tgatggcgaa atggtggatt ttctgggacc ggcttacact ggggcgttcg gatggagcgc
     1681 tggtccgcgt catgatggag gtcggaccgt accaggatat agcggttgcc gatcagcaga
     1741 tgcagcagtt cgttcgcgcc attgttccgg atttgccccg attcattcct gattgaacaa
     1801 ttgccagcag gcgggttggc acaatgcggt ctgtcacctg taccttcatg aaactgtcac
     1861 gtcagtgatt gaaactacgc gagtggcaac aggttccagt gcgttgtcac ggtccgagat
     1921 gcaaaaagcc cttcgaaaga ttttcgaagg gcttttttgt tattcaactg tcatttttcc
     1981 tacatatcat cgtcccactg ccgttggggg gtcgaatgaa acaagttctg caggatctgc
     2041 gcacgggcgc gacttacatt gacaatgtgc catgccccat ggtccagagc ggacatgtgt
     2101 tgattcgcac cacgcaaacg ctggtatcgg ctggcaccga gaaaatgctg gtggattttg
     2161 gcaaggccag tctggttcag aaagcacgcc agcagcccga caaggtgcgc atggtgctgg
     2221 acaagatcag aaccgatggc ctgctaccga cggtggaagc ggtgcgtgcc aagctggatc
     2281 agccgattcc gatgggttac tgcaatgtcg gcgtggtggt tcaatgcggc gccaatgtgg
     2341 aagggttcca gccgggggat cgggtggtgt ccaacgggca tcacgcggaa atggtgaccg
     2401 ttccaaagca cctgtgcgct cgaattcctc cgtccgtgag tgatgaccag gccagtttca
     2461 cggtcatggc ctccatcgcc ctgcagggta tccgtttggc gcagcccgaa ctgggcgaga
     2521 cattcgcggt aatcggactt ggcttggtcg gcttgctcac ggtgcagttg ctcagggcca
     2581 atgggtgccg ggtcattggc attgattttg atgcggggcg tgtctccatg gcccgtcagt
     2641 tgggtgccga gtccattgtg ttgcgggacg gtgccgatcc ggtggcattc gctcagacct
     2701 tcacgggcgg cgttggtgtc gacggcgtac tgattacggc ttccacgcgc agcaatgacc
     2761 cggtgcacca ggcggccacc atgagtcgca agcgtggccg gatcgtgctg gtgggcgtgg
     2821 tggggctgga attgtctcgg gcggatttct atgaaaagga gctgagtttt caagtttcat
     2881 gctcctatgg ccccggtcgc tatgaccctg actacgagca gaaaggcatt gattatccca
     2941 tcggctatgt gcgctggacc gagcaacgca attttgaagc agtgctcgcc gcgatggcca
     3001 gtggagcggt gcatccggaa ggcatgatca cctcacgatt ccggctggat gaggcggccg
     3061 ccgcgtatgc ggccattctg cagggtggag cgctgggcgt agtgctggaa tgtgatcgcc
     3121 agcagccggc cgcgcaagtg ctggcttcaa cgatcgagac caactcagcg cctgccgcat
     3181 taactgcaac catccgcgtt ggctgcattg gatcgggcaa ttatgccagc cgggtgctgt
     3241 tccctgcatt tcgcaaggcg ggtgtcagtt tggccacggt cgcctgcact ggcggcacca
     3301 gtggcaccca tgcagcaaaa aaatttggtt ttctgaaaat taccacagac agcgacactg
     3361 taattgccga ccaccagatc gacctggtga cggtggcaac ccggcatgat acacatgcgc
     3421 gttatgtcaa tgcggccctg ctggcaggca agcatgtatt tgttgaaaag ccgctggcta
     3481 taacgcaggg tgagctgatc agtatcgagc atgcatatac atctacgcgt ggtcacatca
     3541 atcctccgca gctcatgatc ggcttcaatc gccgcttctc ggcgctggtt acgccagtga
     3601 aacaggtgct ggacaccgcc cgggatcccg cctgcatcaa catgaccatc aacgccggcg
     3661 aaattccgcc atcgagctgg atacaggatc tgcaggtggg cggtggc
//
LOCUS       DASUEG010000511         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2017160, whole genome shotgun sequence.
ACCESSION   DASUEG010000511 DASUEG010000000
VERSION     DASUEG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017160"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFX11_09575"
     CDS             <1..403
                     /locus_tag="VFX11_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4938913.1"
                     /translation="FNAPVSEVFAQLSDHEKMGEVLGAKVKRVKDGKGNVNGVGSVRR
                     ITPVPLADFEETVTAFEPNKLIEYTVTKGSPIKNHLGRMVFSESNGKTHLHYTIQFDM
                     KLPIPLSGLLMKTILEKLILSGLQKFGKRYG"
     gene            546..821
                     /locus_tag="VFX11_09580"
     CDS             546..821
                     /locus_tag="VFX11_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938914.1"
                     /translation="MKKIAEEEADVILDSTIIIDGLPFPEDTCVRTEADLSHTDLTLY
                     YQVLEGGIDLDLGMTARKFDGGITSTGRTIFGSLTAKQSLDTTIPLL"
     gene            complement(950..1501)
                     /gene="thpR"
                     /locus_tag="VFX11_09585"
     CDS             complement(950..1501)
                     /gene="thpR"
                     /locus_tag="VFX11_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX4938915.1"
                     /translation="MPDTKPIRCFLGVPVDATFTALLRPLCAALQMQPWAGSLRWVEP
                     HNLHLTLAFLGDQPLELIPSLQRKLLVSLASLPAFQLTGDRLSGFPDAKSSILALEFH
                     TCDALQQLKTRVDETLRQEGLVPDQRPLRPHITLARGSRGQQLPWQSQPCELRLPVGS
                     VTLFQSVAGAAGSDYRALWSIAL"
     gene            complement(1655..2362)
                     /locus_tag="VFX11_09590"
     CDS             complement(1655..2362)
                     /locus_tag="VFX11_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX4938916.1"
                     /translation="MLKSSLTALALGVALVSGSALAYQEGDLILRAGAAAVDPQEDSS
                     QLSINGATTEELLGLDSSAGVDSNTQLGLSLTYMLSNKVGVELLAATPFSHTVTANIE
                     SVGTVEAAEVKHLPPTVSLQYFLLSPESRFQPYAGIGINYTTFFDEEVSADLDAVTQS
                     LGIPGEGSLDLDDSFGFAFELGCDLLIGENLVLNAAVWKADIDTTATFKYDNGTKITA
                     DVGIDPYVYMVAAGYRF"
     gene            2509..2937
                     /locus_tag="VFX11_09595"
     CDS             2509..2937
                     /locus_tag="VFX11_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX4938917.1"
                     /translation="MASLLLTAGLVLAGCSGAPVAKGVAGGNVGGCPGSDNCVSSFTS
                     RSGQKIEPLKFTGSKADAMTKLKAVLAQREDAKIVNEKADYLHVEFTTKLMGFVDDAE
                     FLLATDNIQMRSASRVGYSDFGKNRARLQEIRSAFEPCCN"
     gene            complement(2953..3900)
                     /locus_tag="VFX11_09600"
     CDS             complement(2953..3900)
                     /locus_tag="VFX11_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013796255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HEX4938918.1"
                     /translation="MASILDSVDQRTQLVGENRLELLMFRLQRGNLFAINVFKIQEVQ
                     TMPKLTVMPQSHPNVVGVTHARGRTIPVIDLSAAIGLGPLLDRTNCNIIISEYNRTIQ
                     AFMVKSVDRIVNMNWDEIMPPPKGTGRSHYLTAITKVEKQIVEVIDVEKVLQEISPYN
                     TTVNPALIDNDILTLAEGIEILAVDDSSVGLSQVRETIGQLGIKILSARDGATALRML
                     KKWADEGIEVPRKLAMVITDAEMPEMDGYRLTKEIREDARLRDLYVILHTSLSGSFNN
                     AMIQKVGCDAFLSKFEPDKLALTVQDRIRAYRERYPKNR"
     gene            4021..>4660
                     /locus_tag="VFX11_09605"
     CDS             4021..>4660
                     /locus_tag="VFX11_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4938919.1"
                     /translation="MESPNILLARQPIYDSTLDVTAYELLFRPPAGWVWDGDVATSQL
                     VVNAFAEIGIEKATDNKRAFINFTRQWLLSPPPFDPQLVVIEILESVEPDADVVRSVA
                     QLKRSGYTIALDDFVFAPQWRPLLELADIVKIDVLQHDDAALKTLTGQLREYPLQLLA
                     EKVETYSVFEHCKSLGFQMFQGYFLCRPQNIHGESIPSNKMVVMQLLAEVQDP"
BASE COUNT         1079 a         1308 c         1310 g          963 t
ORIGIN      
        1 cttcaacgca ccggtaagcg aggtgtttgc ccagctttcg gatcacgaga aaatgggcga
       61 ggtgctgggt gcaaaagtga aacgggtgaa ggatggcaag ggcaatgtga acggcgtggg
      121 ttcagtgcgc cgcatcacgc cggtgccgct ggcggacttc gaggaaaccg tgacggcgtt
      181 cgaaccgaac aaactgatcg aatacacagt caccaagggc agcccgatca agaatcacct
      241 gggccgaatg gtattcagcg aaagcaatgg caagacccac ctgcactaca ccatccagtt
      301 cgacatgaaa ttgccaattc cgttgagcgg actgctgatg aaaaccatcc tggagaaatt
      361 gatcctgagc gggctgcaga agttcggcaa gcgctacggc tgatgtcgcg ggtccgggca
      421 gcgatactgc ccggactttc ccttcctgcc ccctccttcc ccctccgttt taatcggtaa
      481 aagttcccgc agccgcaatg ccttctgtgc ttcaattgcc gggctttcaa ctcaggggat
      541 cttgcatgaa aaaaatcgca gaagaggaag cagacgttat tctggattcg accatcatca
      601 ttgacggtct gccctttcct gaggacacct gcgtgcgcac cgaggcagac ctgagccata
      661 ccgacctgac cctgtattac caggtgctcg aaggcggaat cgatctggac ctgggcatga
      721 ccgctcgcaa attcgacggc ggcatcacca gcacaggccg cactattttt ggcagcctta
      781 cggcgaagca aagcctcgac accacaatcc cgctgttata agacatgatg cagttcaacg
      841 tgcccaacac cggcctgtat gtggcggctg atggcagtgc gcgggtttga caggcggttg
      901 aaaacaaatg ttacgcatct tgccggcgcg gccatgtttc aacagcccgt caaagggcaa
      961 tcgaccagag cgcccggtaa tcactgccag ctgcgcccgc tacactctga aacaacgtca
     1021 cgctgccgac tggtaaccgc aattcgcagg gctgcgactg ccacggcagc tgctgcccac
     1081 ggctgccccg cgccagcgtg atgtgcggac gcaatggacg ctgatccggt accagtcctt
     1141 cctgtcgcaa ggtttcgtcc acccgggttt tcaattgttg caacgcatcg caagtgtgaa
     1201 attccagtgc gaggatggaa gatttggcat ccggaaatcc gctgaggcga tcgcctgtca
     1261 gctgaaatgc cggcaaggat gccagggaca cgagcagctt gcgctgcaat gagggtatca
     1321 attccagagg ctggtcaccc agaaaagcca gggtaaggtg cagattgtgc ggttccaccc
     1381 agcgcagaga accggcccag ggttgcattt gcagcgccgc gcacagcggc cgcagcaagg
     1441 ccgtgaaagt ggcatccacc gggacaccga gaaaacagcg gatcggtttc gtatcgggca
     1501 tgctgccatt ttggcacagg cttgccgcca tgacagcagg caaaaaaaag cccctcttgt
     1561 ggaggggccc caaccggatg accctttcgg gtcatttcag agaggaatca gattcaaaga
     1621 gtgcagcacg atgattcaga tgaatggccc gatctcagaa gcgatagccg gcggccacca
     1681 tgtacacgta cgggtcgatg ccaacgtcag ctgtgatttt cgtgccgttg tcatacttga
     1741 acgtggcggt ggtgtcgata tccgctttcc acacggcagc gttcaatacc aggttttcgc
     1801 caatcagcag atcacatccc agctcaaacg caaagccgaa ggaatcgtcc agatccaggg
     1861 aaccttcacc cggaataccc agcgactggg tgactgcatc cagatcagcc gacacttcct
     1921 cgtcaaagaa tgtggtgtag ttgataccga taccagcata gggctggaag cgggattcag
     1981 ggctcaacag gaaatactgc aggctgacgg tgggcggcag atgcttcact tccgcagctt
     2041 ccacagtgcc gacactttca atatttgcgg tgacggtatg ggagaacgga gtagcagcca
     2101 acagctccac gcccaccttg ttgctcagca tgtacgtcag gctcaggccc agctgtgtgt
     2161 tgctgtcgac tccagcggaa ctgtccagac ccagcaattc ttcagtggtc gcgccgttga
     2221 tggacagctg cgagctgtct tcctgcgggt ccaccgcagc tgcacctgcg cgcagaataa
     2281 ggtcaccctc ctggtatgcc agtgcagaac cggaaaccag ggcaacaccc agagccaatg
     2341 ccgtcagaga ggatttcaac ataggtaact accttctatc agtgaataat ctgcaatgag
     2401 gctagtctag gcatacctat ttcggttaca tatgacctgt gtcaatccca ataaaccatg
     2461 ataacgaaag ggtgttccat gctgatacat gtcaacaaaa ctgccactat ggcaagcctg
     2521 ctcttgacgg cgggtctggt tctggccggc tgcagcggcg cgccagtagc caagggtgtg
     2581 gccggaggga atgtgggcgg ctgcccgggc agcgacaact gcgtgagcag tttcacttca
     2641 cggtcagggc agaaaatcga accgctgaag ttcacaggca gcaaggctga cgccatgaca
     2701 aaactgaaag ccgtgctggc gcagcgggaa gatgccaaaa tcgtgaacga aaaggccgat
     2761 tatctgcatg tggaattcac gacaaaactg atgggattcg tggacgacgc ggaatttctg
     2821 ctggccaccg acaacatcca gatgcgttct gcatcgcggg tgggctacag tgatttcggc
     2881 aagaaccgcg cgcggttgca ggaaatccgc agcgcattcg aaccctgctg caactgaagc
     2941 ggcgaccgct gctcagcgat ttttcggata acgttcccga taagcccgga tgcggtcctg
     3001 tacggtcagc gccagcttgt cgggttcgaa tttcgacaga aacgcatcac atcccacctt
     3061 ctggatcatg gcattgttga aactgccgct cagcgacgta tgcaggatca cgtacagatc
     3121 gcgcaatcgc gcatcctcgc gaatttcctt ggtaagccgg taaccgtcca tttccggcat
     3181 ttcagcgtcg gtaatgacca tggccagctt gcgcggcact tcaatgccct catcggccca
     3241 ttttttcaac atgcgcaacg cggtggcacc gtcgcgggcg ctcagaatct tgatgcccaa
     3301 ctgaccgata gtttcgcgta cctgcgacag gcccacgctg gaatcatcca ccgccagaat
     3361 ctcgataccc tcagccaggg tcagtatgtc attgtcgatc agcgccggat tcacggtggt
     3421 gttgtagggt gaaatttcct gcagcacttt ttccacgtca atcacttcaa ctatctgctt
     3481 ttccaccttg gtgatggcag tcaggtaatg gctgcggccg gtgcccttgg gcggcggcat
     3541 gatttcgtcc cagttcatgt tgacgatgcg atccacggat ttgaccatga acgcctgaat
     3601 ggtccggttg tattcggaaa tgatgatgtt gcagttggta cgatccagca gcgggcccaa
     3661 cccgatggca gcactcaggt caatgaccgg tatggtgcgt ccgcgggcat gggtgacgcc
     3721 caccacgttg ggatggctct gcggcatcac cgttagcttg ggcatggtct gcacttcctg
     3781 gatcttgaac acattgattg caaacaggtt tccccgctgc aaccggaaca tcagaagttc
     3841 aagacggttc tcacccacga gctgggtgcg ttgatcgacg ctgtcgagaa tgctggccat
     3901 gactgtttcg gcaacctgtt gataagagtg acaggaaaag tgtagcccac gaagccggca
     3961 ccggcctggg ctacactgat tgaatacgtc ttcgcaattg ccaaatcagg tttgtctgca
     4021 atggaatcac ccaatatcct gcttgcccgc cagcccattt acgacagcac actggacgtt
     4081 actgcctatg agctgctatt ccgtccgccg gccggctggg tgtgggatgg cgatgtggcc
     4141 acctcgcagc tggtagtgaa tgcctttgcc gaaatcggga tcgaaaaggc caccgacaac
     4201 aagcgcgcct tcatcaattt cacgcgccag tggctgctgt cgccaccacc atttgatccc
     4261 caactggtgg tcatcgaaat actggagtct gtggagccgg acgctgacgt ggttcgcagc
     4321 gtggcccaac tgaagcggtc cggctacaca atagcgctgg acgatttcgt gttcgcaccg
     4381 caatggcggc cactgctgga actggcggac atcgtcaaga tagatgtgct gcagcacgat
     4441 gatgcggcgc tgaaaacact gacaggacaa ctgcgggaat atccgctgca attgctggcc
     4501 gagaaagtgg aaacctacag cgtgttcgag cactgcaagt cactgggctt ccagatgttt
     4561 cagggctatt ttctgtgtcg gccacagaat atccatggcg aaagcattcc ctccaacaaa
     4621 atggtagtga tgcaactgct ggcggaagtg caggacccgg
//
LOCUS       DASUEG010000512         5998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1725312, whole genome shotgun sequence.
ACCESSION   DASUEG010000512 DASUEG010000000
VERSION     DASUEG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725312"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFX11_09610"
     CDS             <1..575
                     /locus_tag="VFX11_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938920.1"
                     /translation="SEAQSKHAQARAFSRCLQQHLEKSRLLREQHPTLLQDLGTTLEQ
                     LHTLLPAGRVAVSEKEATFLLSAQALKHCRQSGHACSDLASARDQQGRELTRLRNSLR
                     TMEQQRTFLAAEQQRLLQHWQKEQQQMTQIGRQLMLQNAGNRTIMTELLQMALARSQA
                     QWQALSARAHFELARTQEALLRQRDQGSRM"
     gene            complement(557..1204)
                     /locus_tag="VFX11_09615"
     CDS             complement(557..1204)
                     /locus_tag="VFX11_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938921.1"
                     /translation="MRGLVAAFLLILVSLPLRAETWELARSDETRNIRVYLRDVPHSS
                     YKSFYAVTQVRTRLSSVVAVLSDVPAMPEWIARMKAVKLLRRNADSELWVHARYRLPY
                     PFLEREAVLYSVMKQDPRTKVVTITTRSVPGFVRPQKDRLRLINMHSTWKLTPEKNGD
                     VRIEFWGQGEPGGYVPPLLFNYNLPDEPVQTLRNLRQMLTRDKYQKRELHYIREP"
     gene            complement(1253..2149)
                     /locus_tag="VFX11_09620"
     CDS             complement(1253..2149)
                     /locus_tag="VFX11_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX4938922.1"
                     /translation="MTTTKKRGLGGGRGLNALLSGVKQVQDTVAAVEEGKMPIDGELK
                     KLPVEFMQRGRYQPRRDMDPAALQELADSIKLQGVMQPIVVRPVGEHKYEIIAGERRW
                     RATQLAGLDSIPVIIRDIPDETAIAMALIENIQRENLNPMEEAMAMQRFADEFQFTHQ
                     QIADAVGKSRATVTNLLRLMSLNAEAKSLLEHGDLEMGHAKALLGLPDNLQGKAARAV
                     VAKDLSVRQTEAMVRALLAQKNESAENDKVLDPNVRKLQDDLAEKVGAPVQIEYNAKG
                     KGRLVISYNSLDELDGILAHIK"
     gene            complement(2200..2994)
                     /locus_tag="VFX11_09625"
     CDS             complement(2200..2994)
                     /locus_tag="VFX11_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4938923.1"
                     /translation="MDKILAVTNQKGGVGKTTTSVNLAASLAATKRRVLLIDLDPQGN
                     ATMGSGIDKHELAHSVYDVLTRQCTAEQAIVTGIEAGYHVIGANGDLVAAEVELMNAI
                     GRELRLKQALEAVKDRYDYIIIDCAPSLSMLTVNGLVAADGVVIPMQCEYFALEGLAA
                     LMDTIDQVTQTVNPKLHVTGILRTMFDPRNTLATDVSAELLTHFPDRVFETIIPRNVR
                     LAEAPAHGMPVLLYDKASRGAMSYLALAAELLRRQDAPRETAAETA"
     gene            complement(3043..3666)
                     /gene="rsmG"
                     /locus_tag="VFX11_09630"
     CDS             complement(3043..3666)
                     /gene="rsmG"
                     /locus_tag="VFX11_09630"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX4938924.1"
                     /translation="MSASPENRLRAGVEKLSLSLPDGAMEKLLAYLALLVKWNGAYNL
                     TAIRDPEQMVIKHLLDSLSIAPYVQGGSLIDVGTGAGLPGLVLAIVKPELDVTLLDSN
                     GKKVRFLRQVIADLKIGNASAVQTRVEEFDRQFDLVSSRAFATLADMVNGSRQLLAEK
                     GEFLAMKGVNPTEEIAALPEGFSVRDVVPLTVPFLDEERHLVRIVRN"
     gene            complement(3663..5558)
                     /gene="mnmG"
                     /locus_tag="VFX11_09635"
     CDS             complement(3663..5558)
                     /gene="mnmG"
                     /locus_tag="VFX11_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX4938925.1"
                     /translation="MRYPTPYDVIVIGGGHAGTEAALAAARMGCRTLLLTHNVETLGQ
                     MSCNPAIGGIGKSHLVREIDALGGAMALATDQGGIQFRILNSSKGPAVRATRAQADRV
                     LYKAAIRAMLENQPNLDIFQQAADDLIVEGGAVRGVVTQTGIRFDAKAVVLTTGTFLG
                     GTIHIGLQNHRGGRAGDPPSIALAHRLRELLPNVGRLKTGTPPRIDARSVDFSVMKAQ
                     PGDTPTPVMSFMGSREMHPQQVNCWITQTNLQTHDIIRGGLDRSPMYTGVIDGVGPRY
                     CPSIEDKIHRFADKDSHQIFIEPEGLTTHELYPNGISTSLPFDVQLNLVRSIRGLENA
                     HITRPGYAIEYDYFDPRDLKHTLETKVIANLFFAGQINGTTGYEEAGAQGLLAGLNAG
                     LRAQDKEGWYPTRDEAYLGVLVDDLITLGTKEPYRMFTSRAEYRLMLREDNADQRLTA
                     KGRELGLVDDVRWAAYCEKMDAIERETQRLKSTWVHPGSAMAEAFTTGTGQDLSREYS
                     LMDLLRRPQVSYAQIQALGGSEVSEAVGEQIEIMARYAGYIDRQAEEIERFKRHEETR
                     LPDGFDYDRVSGLSNEVRAKLKQVRPETLAQAARIPGVTPAAVSLLMIWMKKNGVSAH
                     VTEQAAS"
     gene            complement(5708..>5998)
                     /locus_tag="VFX11_09640"
     CDS             complement(5708..>5998)
                     /locus_tag="VFX11_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX4938926.1"
                     /translation="ARQGGGLDVLRETLKTAMGFQSEGAGLFTARRRHLDALERARQS
                     LATGLAQLLDTGAGELLAEDLRVAQEALSEITGEFTADDLLGRIFSSFCIGK"
BASE COUNT         1138 a         1851 c         1807 g         1202 t
ORIGIN      
        1 tcagtgaggc tcagagcaaa catgcacagg cccgcgcctt cagccgttgt ctgcagcagc
       61 acctggaaaa atcccgcttg ttgcgcgagc aacatccaac cctgctgcag gatctgggca
      121 caacgctgga gcaattgcat acgctgctgc ccgccggtcg tgtagctgtg tcggaaaaag
      181 aagccacctt tttgctgtcg gcccaagccc tcaagcattg tcgccagtcc ggtcacgcct
      241 gcagtgatct ggcctctgcg cgtgaccaac agggtcgcga gttgacccgc ctgcgcaaca
      301 gcctccggac catggaacaa cagcgaacct ttctggccgc cgagcagcaa cgcttgctgc
      361 agcactggca gaaagaacaa cagcaaatga cacagatcgg acggcaactg atgctgcaga
      421 atgccgggaa tcgcaccatc atgaccgagc tgttgcagat ggcattggca agatcacagg
      481 cccaatggca agcgctgtcg gcacgtgccc atttcgaact ggcacgcaca caggaagcgc
      541 tgctgcgcca gcgcgatcaa ggctcgcgga tgtagtgcaa ctcccgcttc tgatacttgt
      601 cacgcgtcag catctggcgc aggttgcgca gggtctgtac gggttcatcc ggaaggttgt
      661 agttgaacag caacggcggc acatagccgc ccggctcacc ttggccccag aattcgatgc
      721 gcacatcgcc gtttttttcc ggtgtcagct tccaggtgct gtgcatgttg atcaggcgca
      781 ggcggtcttt ctgcggtcgc acaaaaccgg gcacgctgcg agtggtgatg gtgacgactt
      841 tggtacgcgg atcctgcttc atcaccgagt acagcacggc ctcacgttcc aggaagggat
      901 agggcaggcg ataacgggca tgtacccaca attcactgtc ggcattgcgg cgcagcagct
      961 tgaccgcctt catgcgggcg atccattccg gcatggccgg cacatcggac agcacagcga
     1021 ccaccgagct caggcgtgtc ctgacttgcg tgaccgcata gaacgatttg taggaagaat
     1081 ggggaacatc gcgcagataa acgcggatgt tgcgggtctc gtcgctgcgt gccagctccc
     1141 aggtctctgc ccgcaagggc agcgacacca ggatcagcag gaatgcagca accagtccac
     1201 gcacggttaa cgtcctgtcg ggcggaatac cgccggcgtg ggcggaatcc gtttacttga
     1261 tgtgagcgag aatgccgtcc agctcatcca gcgaattgta gctgatgacc aggcgaccct
     1321 tgcccttggc gttgtactcg atctgtaccg gtgcgccaac cttctcggcc aggtcatcct
     1381 gcagcttgcg cacgttcggg tccagtacct tgtcgttctc ggcgctttca ttcttttgcg
     1441 caagcagcgc acgcaccatg gcttcggtct ggcgaaccga cagatccttg gccaccacgg
     1501 cacgggcggc cttgccctgc aggttgtcgg gcaggccgag cagcgccttg gcatggccca
     1561 tttccagatc cccatgttcc agcagggact tggcttctgc attgagcgac atcaagcgca
     1621 ggagattggt gacggttgcg cgggacttgc ccacggcatc ggcaatctgc tggtgagtga
     1681 actggaactc gtcggcaaag cgctgcatgg ccatcgcttc ttccatcggg ttcaggtttt
     1741 cacgctggat gttctcgatg agcgccatgg caatggcggt ttcatcggga atgtcgcgga
     1801 tgatgacggg aatgctgtcg agacctgcca gctgggtggc gcgccagcga cgttcaccgg
     1861 caatgatctc gtacttgtgc tcacccaccg gacgtacaac gatgggctgc atcacaccct
     1921 gcagcttgat ggagtcggcc agttcctgca gcgcggcggg gtccatgtcg cggcgcggct
     1981 gatagcggcc acgctgcatg aattcgacgg gcagcttctt cagctcgcca tcaatcggca
     2041 tcttgccttc ttcgaccgcc gccaccgtgt cctgtacctg cttcacaccg gagagcaggg
     2101 cattaaggcc acggccgccg ccgagtccgc gcttcttggt agtcgtcata tccatttcac
     2161 cattacccct tccaactcag gaaaggaaaa aaatcagaat caagctgtct cggctgcggt
     2221 ttcacgcggg gcatcctggc ggcgcagcaa ctcggcggcc agtgccagat aggacatggc
     2281 gccacgcgag gccttgtcat aaagcagtac cggcatgccg tgggccggtg cttcggccaa
     2341 gcgcacatta cgcgggatga tggtttcgaa cacgcggtcc gggaagtgcg tcaacagctc
     2401 ggcggacacg tcggtggcca gcgtgttgcg cgggtcgaac atggtgcgca ggatgccggt
     2461 cacgtgcagc ttggggttca ccgtctgcgt cacctggtca atggtgtcca tcaatgcggc
     2521 caggccttcc agggcgaagt attcacactg catgggaatg acaacgccgt cagcagccac
     2581 cagaccgttt acggtcagca tgctcagcga cggcgcgcaa tcgatgatga tgtaatcgta
     2641 acgatccttc actgcttcca gcgcctgctt caggcgcagc tcgcggccga tggcattcat
     2701 cagttcgact tcggcagcga ccagatcgcc attggcgcca atgacgtgat agcccgcttc
     2761 aataccggtg acgatggcct gctcggcggt gcactggcga gtgagcacgt cataaacgct
     2821 gtgcgcaagt tcatgcttgt cgatgcccga gcccatggtc gcattgccct gcggatcaag
     2881 atcgatcagc agcacgcggc gcttggtcgc agccagtgag gcggccaggt tgacactggt
     2941 ggtcgtcttg cccacgccac ctttctgatt ggtcacggcc agtatcttgt ccatgtcttc
     3001 cccactgtct tgttttccgt ctccccaaca cggggatacg gatcaattgc gcacgatacg
     3061 caccaggtgc cgctcttcgt ccaggaatgg caccgtgagc ggtacaacgt cccgcaccga
     3121 aaaaccttcg ggcagggccg cgatttcttc tgtcgggttc acacccttca tggcgagaaa
     3181 ctcgcccttc tccgccagca attgccggct gccgttgacc atgtcggcga gcgtggcaaa
     3241 agcccggctg ctgaccaaat caaactggcg gtcaaactct tccacacgtg tctgcactgc
     3301 actggcgttg ccgatcttca aatccgcaat cacctggcgc aggaagcgca ctttcttgcc
     3361 gttgctgtcg agcaacgtta catccagttc cggcttcacg atggccaaca ccaggcccgg
     3421 caaaccggca ccagtgccca catcgatgag cgagccgccc tgcacatagg gcgcgatgct
     3481 caggctgtcg agcaggtgct tgatcaccat ctgctccgga tcacggatgg cggtcaggtt
     3541 gtacgcgcca ttccatttca ccagcaacgc cagataagcc agcaactttt ccatcgcacc
     3601 gtccggcagg gacagcgaca acttttctac accggcgcgc agccggtttt caggtgatgc
     3661 actcacgagg ccgcctgttc cgtcacgtgg gcactcacgc cgtttttctt catccagatc
     3721 atcagcagcg agaccgctgc tggtgtcaca ccgggaatac gcgcggcttg ggccagcgtt
     3781 tccgggcgca cctgcttcag cttggcacgc acttcgttcg agagaccgct gacgcggtcg
     3841 taatcaaaac cgtccggcag acgggtttct tcatgacgct tgaagcgttc gatttcctcg
     3901 gcctggcgat cgatatagcc ggcataacgc gccatgattt caatctgttc accgaccgct
     3961 tcgctgacct cgctgccacc gagcgcctgg atctgcgcat agctcacctg cggacgacgc
     4021 agcaggtcca tcagcgaata ttcgcgcgac aggtcctggc cggtgcccgt ggtgaatgcc
     4081 tcggccatgg ccgagcccgg gtgcacccag gtggatttca ggcgctgcgt ttcgcgctcg
     4141 atggcgtcca tcttttcgca gtacgccgcc cagcgcacat catcgaccag gccgagttca
     4201 cggcccttgg ccgtcaggcg ctggtcggca ttgtcttcgc gcagcatcaa gcggtattcc
     4261 gcgcgcgagg tgaacatgcg gtagggttct ttcgtgccca gcgtgatgag gtcgtcgacc
     4321 agcacgccga gataagcctc gtcacgtgtc ggataccagc cttctttgtc ctgcgcacgc
     4381 aggccggcat tgagaccggc gagcaggcct tgtgcaccgg cttcttcata accggtggta
     4441 ccgttgatct ggccggcaaa aaacaggttg gcgatgactt tggtttccag cgtgtgtttc
     4501 aaatcacgcg gatcgaaata gtcgtattcg atggcgtagc ccggacgcgt gatgtgcgcg
     4561 ttttccaggc cccgaatgga gcgcaccaga ttcagctgca catcaaacgg caaggacgtg
     4621 gaaatgccgt tggggtacag ctcgtgtgtc gtcaggcctt cgggctcgat gaaaatctgg
     4681 tgcgagtcct tgtcggcaaa acggtggatc ttgtcttcga tcgacgggca ataacgcgga
     4741 cccacgccgt cgatcacgcc ggtgtacatc ggcgaccggt cgagaccgcc gcggatgatg
     4801 tcgtgcgtct gcagattggt ctgcgtaatc cagcagttca cctgctgcgg gtgcatctcg
     4861 cgcgaaccca tgaaggacat caccggcgtc ggcgtgtcgc ccggctgcgc cttcatcacg
     4921 gagaagtcca ccgagcgggc atcgatacgt ggtggtgtgc cggtcttgag gcggccgaca
     4981 ttcggcagca gctcgcgcag gcggtgggcc agcgcaatag agggcggatc accggcacgg
     5041 ccaccgcggt ggttctgcag gccgatgtgg atggtgccgc ccaggaaggt gccggtggtg
     5101 agcaccacgg ccttggcatc aaaacggata ccggtctggg tgaccacacc acgcacggcc
     5161 ccaccttcca cgatcaggtc gtcggcggcc tgctggaaga tgtcgagatt gggctggttt
     5221 tccagcatgg cgcggatcgc agccttgtac agcacgcggt cggcctgggc acgggtagca
     5281 cgcacggccg ggcccttgct ggaattgagg atgcggaact ggatgccgcc ctggtcggtg
     5341 gccagcgcca tggcgccgcc cagggcgtcg atctcgcgca ccagatgcga tttgccgata
     5401 ccaccgatgg ccgggttgca gctcatctgg cccagagttt ccacgttatg ggtgagcagc
     5461 agcgtgcgac agcccatgcg tgcagccgcg agcgcggctt cggtgccggc atggccaccg
     5521 ccgatcacga tcacgtcata gggagtcggg taacgcatgg tcgcctggct tcatgaaaaa
     5581 aagggggaac gtagtatacg ggttgaccgg gcaaaaagaa ccggggcgtt cactttttga
     5641 ccaagtttcc gtccggaagc tgtcgcgctt ctaccctgaa actggccttt tcgctgttag
     5701 tttgtactta cttaccgata cagaagctgg aaaagatgcg tcccagcagg tcgtcggccg
     5761 tgaactcgcc ggtgatttcc gacagtgcct cctgcgccac gcgcaggtct tcggccaaca
     5821 actcccctgc cccggtgtcc agcaactggg ccaggccagt ggccagtgac tggcgcgccc
     5881 gctccagggc atccagatgg cgacggcggg cggtgaacag gccagcccct tcgctctgga
     5941 aacccatcgc tgtcttcagg gtttcacgca atacgtccag tccgccgcct tgccgggc
//
LOCUS       DASUEG010000513         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1614860, whole genome shotgun sequence.
ACCESSION   DASUEG010000513 DASUEG010000000
VERSION     DASUEG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614860"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFX11_09645"
     CDS             <1..123
                     /locus_tag="VFX11_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX4938927.1"
                     /translation="GMNFDVMPELRWHYGYPATLAVMAVLCGLLFRRLRRAGWL"
     gene            212..1084
                     /gene="tesB"
                     /locus_tag="VFX11_09650"
     CDS             212..1084
                     /gene="tesB"
                     /locus_tag="VFX11_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX4938928.1"
                     /translation="MTQVMQEVLDLLDLETIEAGLFRGPSRNIVGKNVFGGQVLGQAL
                     MAAGRTVEGRLAHSLHGYFLRAGDTKAPIVYQVEKIRDGKSFATRNVKAIQHGEIIFI
                     MSASFAVVEEGLEHQSDMPAVEGPDGIPSETELRQKVAPMIPEKIRDAFARERPIEIR
                     PINPVNPFAPVKQEPKRFHWMRAQSRLPDDPFLHQCILAFASDFALMGTAMMPHGVSF
                     MQNNLQAASIDHAMWFHRSFRVDEWLLYEMDAPNASSSRGMNRGLIYSQDGRLVASTA
                     QEGLMRLREVPEKY"
     gene            complement(1114..>2285)
                     /locus_tag="VFX11_09655"
     CDS             complement(1114..>2285)
                     /locus_tag="VFX11_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4938929.1"
                     /translation="IGLILSGGGARAAYQVGVLRAIGEILPPGCHNPFHIICGTSAGG
                     LNATGLATHADCLRDGISMLETVWSEFRTNQVYRTDWFGVLHRAARWIWTMAFGRLRR
                     DLPVSLLDNSPLRDLLDAHLPLERIQHAIDNGLLEALCITASGYSTGESVSFFQGAPH
                     LSGWNRSRRVGIRTAISIDHLLASSAIPMLFPAVRVNREYFGDGAIRQHAPISPALHL
                     GAERVMVIGVGGQSTEAERERSLSYPSIAQIVSHILSSSFVDSLESDIERLTRINRTL
                     TLIPEDVRHMSSLRPVDVMVISPSAQVLDTIAMRHADKLPRSIRMFVRGSEATQRSGS
                     GVLSYLLFEGAYCQDLMQLGYSDGCARKEELKRFFSIEEAEKPASNVVEFRRSEA"
BASE COUNT          446 a          630 c          753 g          456 t
ORIGIN      
        1 ggcatgaact ttgacgtgat gcccgagctg cgctggcact acggttatcc ggcgacgctg
       61 gcggtcatgg cggtgctgtg tggtctgctg ttccggcgct tgcgccgggc cggttggcta
      121 tagtgtcagt gacggtttcc ctcaaacagg ttctggtcta ggctttgcga ccagtttgac
      181 agagagccgg attgacagtt gaggtaatgc catgacccag gtaatgcagg aagtgcttga
      241 tctgctggat ctcgagacaa tcgaggccgg gcttttccgc ggccccagcc gcaatattgt
      301 cggcaagaac gtgtttggcg ggcaggtgct ggggcaagcg ctgatggcgg ccgggcgcac
      361 ggtggagggg cgactggccc attcgctgca tggttacttc ctgcgtgccg gcgatacgaa
      421 ggcccccatt gtctatcaag tggaaaaaat ccgtgatggc aagagtttcg ccacacgcaa
      481 tgtaaaagcc attcagcatg gcgagatcat cttcatcatg tcggcatcgt ttgcggtggt
      541 ggaggagggg ctggagcacc aatcggacat gcctgccgtt gaaggacctg acggtattcc
      601 gagcgaaacc gaattgcgtc agaaggtggc gccgatgatt ccggaaaaaa ttcgcgatgc
      661 ctttgcccgg gagcggccaa tcgagatccg tccgatcaat cccgtgaatc ccttcgcgcc
      721 ggtgaaacag gagcccaagc gttttcactg gatgagggcg cagagtcgcc tgccggatga
      781 tcccttcctg catcagtgca tcctggcgtt tgcctccgac tttgccttga tgggaacggc
      841 gatgatgccg catggcgtgt cgttcatgca gaacaacctt caggcggcca gcattgatca
      901 cgccatgtgg ttccaccgtt catttcgcgt ggatgaatgg ctgctgtatg aaatggatgc
      961 ccccaacgca tccagctcgc gcggtatgaa tcggggtctg atctattcgc aggatggccg
     1021 cctggtggcc agcacggcgc aggaaggcct gatgcgcttg cgcgaagtgc ccgagaaata
     1081 ctgaaatatc tggcggccag ggatggccac ggttcaggct tcgctacgcc tgaactccac
     1141 aacattgctg gccggctttt cggcctcttc aatgctgaag aagcgcttca gctcctcttt
     1201 tcgtgcacat ccgtcgctgt agcccagctg catcaggtcc tggcaatagg cgccctcgaa
     1261 cagcaggtag ctcagtaccc ctgatcctga ccgctgtgtg gcttcggagc cgcgtacaaa
     1321 catccggatg cttcgtggca gcttgtcggc atggcgcatg gcaatcgtgt ccagcacctg
     1381 cgctgacggt gagatgacca tgacatccac cgggcgcagc gagctcatgt gcctgacgtc
     1441 ctccggaatc agggtaagag tgcggttgat gcgggtcagg cgctcgatgt cggactccag
     1501 actgtctaca aaggaactgc tcaggatgtg gctgacgatc tgggcgatgc tggggtagct
     1561 gagagagcgt tcgcgttcag cctcggtgga ttggccaccg acaccgatga ccatgacgcg
     1621 ctcggccccc aggtgcaggg cagggctgat cggagcgtgc tgccggatgg cgccgtcacc
     1681 gaagtattcg cggttcacgc gcactgcggg gaacagcatg ggaatagcag aggaggcgag
     1741 caggtggtcg atggagatgg cggtgcgaat gcccacacgg cgtgaccggt tccagccgct
     1801 gaggtgagga gcgccctgga agaagctgac ggattcgccg gtcgaatagc cggatgccgt
     1861 gatgcacagg gcttcaagaa gcccgttgtc gatggcgtgc tggatgcgct ccagcggtaa
     1921 atgggcatcc agcagatcgc gtagcgggct gttgtccagc agggagacgg gcaggtcccg
     1981 gcgcaggcgg ccgaaggcca tggtccatat ccagcgggcg gcacggtgca gcaccccgaa
     2041 ccagtcggtg cgataaacct ggttggtgcg aaactcgctc cagaccgttt ccagcatgct
     2101 gatgccgtct ctcaggcagt cggcatgggt ggccagtccg gtggcattga gaccgccagc
     2161 cgaagtgccg cagataatgt gaaagggatt gtggcagccg ggaggaagga tttcgccgat
     2221 ggcacgcagc acaccgacct gataggccgc tcttgccccg ccaccggaca ggatgagacc
     2281 aatgt
//
LOCUS       DASUEG010000514         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1286871, whole genome shotgun sequence.
ACCESSION   DASUEG010000514 DASUEG010000000
VERSION     DASUEG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286871"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFX11_09660"
     CDS             <1..472
                     /locus_tag="VFX11_09660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4938930.1"
                     /translation="NNVKYLKYLERGRVEHMIHTPWLREMFNRGFKALIANTEISYVK
                     ELKPFQYFKVETRISAWDDKYVYMEQLFTYKRTVFTAAVIRMAMVSTRTGKRVAPSHM
                     LREAFPDLQSPPVPDSARALNQLVQAQRGETQAIADAHAAQDDINTNKIQEAKP"
     gene            469..1236
                     /locus_tag="VFX11_09665"
     CDS             469..1236
                     /locus_tag="VFX11_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4938931.1"
                     /translation="MTEQVLTEIQDGVIIIRLNRADKKNALTGAMYNGISDAFDKLES
                     DNSLRAGLITGTTDCFTAGNDLVDFLSASSIGGDSPVGRFLRTLPALTKPLVAAVNGP
                     AVGVGTTMLLHCDLVYAAPGAKFQMPFVNLALCPEAGSSLLLTQRLGYRRAAELLMLC
                     DAFTADEGKEMGIVSQVLPAEEYQGFALQKAKQLAKQPPASIRATKQLMRMGNAAMLK
                     QVMDAEANQFSAMLAGAEAKEAFTAFMEKRRPDFSRF"
     gene            1427..2209
                     /gene="blaOXA"
                     /locus_tag="VFX11_09670"
     CDS             1427..2209
                     /gene="blaOXA"
                     /locus_tag="VFX11_09670"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX4938932.1"
                     /translation="MNYFLSALALLISFPLFAVEWHDSPAVSQLFANAGAQGTFVLYD
                     VSAKKLAGHNYARAETRFIPASTFKIPNSLIGLSVGAVKDVNEVLPYGGQPQPFKQWE
                     KNMDLREAIKVSSVPIYQELARRIGQQRMNNAVNRLQYGNGQIGTVVDRFWLDGPLKI
                     SAIEQAQFLALLAQEKLPFPRSAQNRVNEITKLETTPEWTLHGKTGWVTFSKPNVGWW
                     VGWVEKQGKIYAFALNMDMAGEVDLPKRVELGKASLMALGVM"
     gene            2220..>2500
                     /locus_tag="VFX11_09675"
     CDS             2220..>2500
                     /locus_tag="VFX11_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938933.1"
                     /translation="MSVMIALHLLSAVIWVGGMFFAYMALRPVAASLLEPPVRLTLWA
                     QVFTRFFPWVWASVLLLLGTGLWMTFGIFGGMKYVGIHVHIMLLLGLIM"
BASE COUNT          529 a          780 c          730 g          461 t
ORIGIN      
        1 gaacaacgtg aagtacctga agtacctcga acgcggccgg gtggagcaca tgatccacac
       61 gccctggctg cgggaaatgt tcaaccgcgg cttcaaggcg ctgatcgcga acaccgagat
      121 cagctacgtg aaggaactga agccattcca gtatttcaag gtggaaaccc gcatcagcgc
      181 ctgggacgac aaatacgttt acatggagca gcttttcacc tacaaacgca ccgtgttcac
      241 cgccgccgtc atccgcatgg ccatggtcag caccaggacc ggcaagcggg tggcgccgtc
      301 gcacatgctg cgggaagcgt ttcccgatct gcaatcgccg ccggtgccgg acagtgcccg
      361 cgccctcaac cagctggtgc aggcccagcg cggcgaaacc caggccattg ccgatgccca
      421 cgcggcacaa gacgacatca ataccaacaa gattcaggaa gccaaaccat gaccgagcag
      481 gtactcacgg aaatccagga cggcgtgatc atcatccgcc tcaaccgcgc cgacaagaaa
      541 aacgccctca ccggcgccat gtacaacggc atctccgacg cgttcgacaa actggaaagc
      601 gacaacagcc tgcgcgcggg cctgatcacc ggcaccaccg actgcttcac cgccggcaac
      661 gatctggtgg atttcctgtc ggcctcgtcc atcggtggcg attcccctgt gggccgcttc
      721 ctgcgcaccc tgcccgccct gaccaagccg ctggtggcag cggtgaatgg cccggccgtg
      781 ggcgtgggca ccaccatgct gctgcactgc gatctggtgt acgcggctcc cggcgccaaa
      841 ttccagatgc cgttcgtgaa cctggcgctg tgtcccgagg ccggctccag cctgctgctg
      901 acgcaacggc tgggctaccg ccgcgccgcc gaactgctga tgctgtgcga tgcgtttact
      961 gcggacgaag gcaaggaaat gggcatcgtg agccaggtgc tgccggcgga ggaataccag
     1021 gggttcgcat tgcagaaggc caaacagctg gccaagcagc caccggcgtc gatccgcgcc
     1081 accaagcagc tgatgcgcat gggcaacgcc gccatgctga agcaggtgat ggatgcggaa
     1141 gccaaccagt tcagcgccat gctggccggt gccgaggcga aagaagcctt cactgccttc
     1201 atggaaaagc gcagaccgga tttttcgcgc ttctgaagcg atggtccacg cagaacactg
     1261 aattaacaca gaaaagcccg ttcatcgcgg gcttttctgt gcttgcggat gggtgcgaca
     1321 gccagtccac ggcaaaccgg ctggcctgca gccgattttg attctgtttt gctaccaact
     1381 gtgtcaggct cctgcagtca tcttcgaccg ccgcctccgg gacaccatga actatttttt
     1441 gtccgccctt gccctgctga tctccttccc cctattcgcc gttgaatggc atgacagccc
     1501 cgccgtcagc cagctgtttg ccaatgccgg tgcacagggc accttcgtcc tctacgacgt
     1561 cagcgccaaa aaactcgccg ggcacaacta cgcacgggca gaaacccgct tcattcccgc
     1621 ttccaccttc aaaattccca acagcctgat cggcctgtcg gtaggagccg tgaaggacgt
     1681 caatgaagtg ctgccttatg gcggacagcc gcaacccttc aagcagtggg aaaaaaacat
     1741 ggacctgcgc gaggcgatca aggtttccag cgtaccgata taccaggaac tcgcgcgccg
     1801 gattggccag cagcgcatga acaacgccgt caaccggctg caatacggca acggccagat
     1861 cggcacggtg gtggatcgct tctggctgga tggcccgctg aaaatcagcg ccatcgaaca
     1921 ggcgcaattc ctggcgctgc tggcgcagga aaaactgccc ttcccgcgct cggcgcaaaa
     1981 ccgcgtcaat gaaatcacca aactggaaac cacgccggaa tggaccctgc acggcaagac
     2041 cggctgggta accttcagca agcccaacgt cggctggtgg gtggggtggg tggagaagca
     2101 gggaaaaatt tacgcgtttg cgctgaacat ggatatggca ggggaagtgg acctgccgaa
     2161 acgggtggaa ctggggaaag ccagtttgat ggcgctgggc gtaatgtgag gaacccacca
     2221 tgtctgtgat gattgccctg caccttctct ccgccgtcat ctgggtgggc ggcatgtttt
     2281 tcgcctacat ggcgctgcgc cctgtcgccg ccagtttgct ggaaccaccg gtacgcctca
     2341 ccttgtgggc gcaggtgttc acgcggtttt tcccctgggt gtgggcatcg gtgctgctat
     2401 tgctcggtac cggattgtgg atgacattcg gcattttcgg cggcatgaaa tatgtcggca
     2461 tccatgttca catcatgctg ttgctcggcc tgatcatgat
//
LOCUS       DASUEG010000515         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_634087, whole genome shotgun sequence.
ACCESSION   DASUEG010000515 DASUEG010000000
VERSION     DASUEG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634087"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1316)
                     /locus_tag="VFX11_09680"
     CDS             complement(255..1316)
                     /locus_tag="VFX11_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4938934.1"
                     /translation="MDFPFPIIDPHIHQWDLLHTPRILSLPRRLLGWNRWLYETTLRF
                     GAKPGDRDYVGRTDYVAWDYLPQQYRQDVGPLPVTQVVHVEAEWRDRSPLGPAGETAW
                     LDRLFEGDTGPQLGGIVAYADLRSPRLNELLTAHRAASGKLRGIRQMLAYDTDQGIMR
                     FCDRQGLAGDEQWRRGLAQLQPLGLSFDAWVFHHQLNELQRLAKAFPQQQFVLDHMGT
                     PIGLGGPFASYGRNAMAREVILKTWQYGMADLAECPNVMVKLSGFFMPVVGWGFEKRE
                     QPPSAQELLDAFAPACRFVLEQFGVERCLFASNFPMDKVSLSLQSLYELYAATVADLP
                     LDDRRKLFHDNAQRIYRLA"
     gene            complement(1385..2509)
                     /locus_tag="VFX11_09685"
     CDS             complement(1385..2509)
                     /locus_tag="VFX11_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase/peptidase C39
                     family protein"
                     /protein_id="HEX4938935.1"
                     /translation="MSAKYNPAEIYIRPATEDDLPGLVRLEELCFETDRMSKRSFRMQ
                     IQSDHNLLLVADHNGLVLGYILIFMRLGVSLARLYSIAVDPDAHGCGIGNRMLDVAEQ
                     KAAKAGRIFMRLEVRKDNDSAIALYKRRGYRQFGYYQDYYEDHADALRYQKRIVQHGE
                     SSGRREVPYYQQSTGFTCGSAVLMMAMSALRPGMAPDKRTEFQIWREATTIFMTSGHG
                     GCGPHGLAYAASKRNFHADVYVNQEGPLFLEGVRNPVKKEILELVHQDFLEKLKDADV
                     DVYTEWLTLYQLERALDDKAIPLVLISTYRFDRRKVPHWVVMVDMDDQFVYIHDPDVN
                     DEEGQNEFDNHYIPIPRSSFDRMFQFGQTRLRCAVILRDV"
     gene            complement(2509..>3150)
                     /locus_tag="VFX11_09690"
     CDS             complement(2509..>3150)
                     /locus_tag="VFX11_09690"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX4938936.1"
                     /translation="ADGDWQRADATLAQYMNQHGQFNYEVAASGGQYEFADGLFYGGH
                     APAQARRNLEWVLQRWDWSNREVAVIDLHTGLGPYGYGELICDHPLGSEGLHTARRWF
                     GASVTVPEEGNSCSVPKSGLVDYAWHAILGRNSCYVTLEYGTFPIAGLLRTLREDHAV
                     RKPGPHDWNDPASARVRDRLRLHFYPGESQWQTLVLLRARQVVQLACTGLLQN"
BASE COUNT          615 a         1030 c          923 g          582 t
ORIGIN      
        1 tgctggtgca ctgcgccgcc aacatgcggg tgtcggcctt catgttccgc tatcggacgc
       61 agatattggg ggaagacaag gcattggcgg agcaggacct gcaccgtatc tggaaaccgt
      121 tcggcgcctg ggccgctttc atagaacgcg agatcgagcg accggcgccg cgctgacgac
      181 taccggcaac attttccgac cagtcgaaac tttattttcc aaatagctgc tgattttccg
      241 aatgccagac gctatcacgc cagccggtaa atccgctgcg cgttgtcatg aaacagcttg
      301 cgccggtcat ccagcggcag atccgccacg gtggcagcgt acagctcata cagcgactgc
      361 agactcagcg acaccttatc catggggaaa tttgacgcga acaggcagcg ctccacgccg
      421 aactgctcca gcacgaaacg gcaggcgggc gcgaaggcat ccagcaattc ctgcgctgac
      481 ggcggctgtt cgcgtttctc gaaaccccag cccaccaccg gcatgaaaaa gccgctcagc
      541 ttcaccatca cgttgggaca ttccgccaga tccgccatgc cgtactgcca ggttttcagg
      601 atcacctcgc gggccatggc attgcggcca taggacgcaa acggaccacc caggccgatg
      661 ggtgtgccca tgtgatcgag cacgaactgc tgctgcggaa atgccttggc cagccgctgc
      721 agttcgttca gctggtggtg gaagacccag gcgtcgaagc tcaggccgag cggctgcagc
      781 tgcgccagac cacgccgcca ctgctcatca cccgccagcc cctgccggtc gcaaaaacgc
      841 atgatgccct ggtcggtgtc gtacgccagc atctggcgga tgccccgcaa cttgccgctg
      901 gccgcccggt gcgccgtgag cagttcattc aatcgcggag aacgcaggtc ggcataggcg
      961 acaatgccgc ccaattgcgg ccccgtgtcg ccctcgaaca ggcggtccag ccacgccgtt
     1021 tcaccggcgg gcccgagggg cgaacggtcg cgccactccg cttccacatg caccacctgg
     1081 gtgacgggca gcggcccgac gtcctggcgg tactgctgcg gcagataatc ccaggccaca
     1141 tagtcagtcc gccccacata atcgcgatcg ccgggcttgg ccccaaaccg cagagtggtt
     1201 tcatacagcc agcggttcca acccagcagc ctgcgcggca ggctgaggat gcgcggcgta
     1261 tgcaacagat cccactggtg aatatgcgga tcaatgatcg gaaagggaaa atccacagac
     1321 gcggcatctc gttttgttat ttgtgcacga tggtaccaaa ttcattctgc cgggagaacc
     1381 ctgctcacac atcccgcagg atcactgcgc agcgcagccg ggtctggccg aactggaaca
     1441 tgcggtcgaa actgctgcgt ggaatgggaa tatagtggtt gtcgaactcg ttctgccctt
     1501 cctcatcgtt cacgtccggg tcgtggatgt aaacgaactg gtcgtccata tccaccatca
     1561 ccacccaatg gggaaccttg cgccggtcaa agcggtaagt gctgatcagc accagtggaa
     1621 tcgccttgtc atccagggcc cgctccagct ggtacagggt cagccactcg gtgtagacat
     1681 cgacgtcggc gtccttcagc ttttccagga aatcctgatg caccagctcc aggatttcct
     1741 ttttcaccgg gttgcgcacc ccttccagga acagcggccc ttcctggttc acgtacacat
     1801 cggcgtggaa gttgcgcttg ctggcggcgt aagccagacc gtgcgggccg caaccgccat
     1861 ggccggaggt catgaaaatg gtggtggcct cgcgccagat ctggaattcg gtgcgcttgt
     1921 ccggcgccat gccgggccgc agcgcgctca tggccatcat cagcaccgcc gagccgcagg
     1981 tgaaaccggt gctctgctgg taataaggca cttcccgccg gccgctgctt tcgccgtgct
     2041 gcacgatccg cttctggtag cgcagcgcgt cggcatgatc ctcataataa tcctggtagt
     2101 agccgaactg gcgatagccg cgccgcttgt acagggcgat ggccgaatca ttgtccttgc
     2161 gcacttccag gcgcatgaag atccggccgg ctttcgccgc cttctgctct gccacatcca
     2221 gcatccggtt gccaataccg caaccatggg cgtcggggtc caccgcgatg gaatacagcc
     2281 gcgccaggct cacccccagg cgcatgaaaa tcaggatgta gcccagcacc agaccattgt
     2341 gatccgccac cagcagcagg ttgtgatcgc tctggatctg catgcggaaa ctgcgcttgc
     2401 tcatgcggtc agtttcgaaa cacagctctt ccagccgtac caggccgggc agatcgtcct
     2461 cggtggcggg gcgaatgtaa atttcagcag ggttgtattt ggcgctcatc aattttgcag
     2521 caatcctgta caggccagtt gaaccacctg gcgcgcccgc agcagcacca gggtctgcca
     2581 ctgggattcg ccggggtaga aatgcagccg caggcgatcg cgcacccggg cagaggcagg
     2641 gtcattccag tcgtgggggc cgggcttgcg cacggcatgg tcctcgcgca gggtacgcag
     2701 cagaccggca atagggaagg tgccgtattc cagcgtcaca taacagctgt tgcggcccag
     2761 tatggcgtgc caggcgtaat ccaccaggcc cgatttcggc accgagcagg aattgccttc
     2821 ctccggcacc gtcaccgacg cgccgaacca gcgccgcgcg gtgtgcagac cctcgctgcc
     2881 cagcgggtgg tcgcagatca gttcgccgta accgtaaggc cccagccccg tgtgcagatc
     2941 gatcaccgcc acttcacggt tcgaccagtc ccagcgctgc agcacccatt ccagattgcg
     3001 gcgtgcctgc gccggcgcat ggccaccgta gaacagtcca tccgcaaatt catactggcc
     3061 accgctggcc gccacttcat agttgaactg accgtgctga ttcatgtact gcgccagcgt
     3121 cgcgtcggcc cgctgccagt cgccgtcggc
//
LOCUS       DASUEG010000516         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1150911, whole genome shotgun sequence.
ACCESSION   DASUEG010000516 DASUEG010000000
VERSION     DASUEG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150911"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFX11_09695"
     CDS             <1..522
                     /locus_tag="VFX11_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX4938937.1"
                     /translation="PGGKASRPGDIVTTMSGQTVEILNTDAEGRLVLCDALTYIERYE
                     PASVVDIATLTGAIIVSLGAHATGMFSNNDGVVEQLKQAADYTQDRVWHLPIWEDYQS
                     QLDSPFADMANIGGPKAGSITAACFLSRYTKKYPWAHLDVAGTSFLGQPKGATGRPVP
                     LLSEYLISQTRKK"
     gene            519..953
                     /locus_tag="VFX11_09700"
     CDS             519..953
                     /locus_tag="VFX11_09700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX4938938.1"
                     /translation="MTQVDFYILPEDNSLEPLLYAARLIEKAFRRGHRLYVHTASAAD
                     TQALSDLLWQRDGSFLAHGALHDTSSAAPGEAIVVSHLGNPGQHSDVLVNLAGTVPPF
                     CQNFQRIAEIVPGKPESRSQSRENFRHYKEKGYSLKTHNIQP"
     gene            963..1118
                     /locus_tag="VFX11_09705"
     CDS             963..1118
                     /locus_tag="VFX11_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938939.1"
                     /translation="MNHGDSKTSLKQQEKQKMLDELESLSRMLDENLDDEDDTPPRPV
                     PEDIPVL"
     gene            1125..1991
                     /locus_tag="VFX11_09710"
     CDS             1125..1991
                     /locus_tag="VFX11_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938940.1"
                     /translation="MDDVPVLNERFLDEEPEIIAPPPKPKPAQKTDSVDQSAARFNTA
                     PAPDFSGLLDDSFLDNDPLEISAAVRQHKPVPEPPLMREALQLDPESVRPAAPVATAR
                     PAQAESPAPQPRASAPVPPPVTARTAVPEPQAARPEPVRPPAAPAATTKPAVSAPLPS
                     ASQAVSAPAPAAAPVPHTAASITQPLNRFQTSSKNENPFLPKSTLDKIRENHQQYDAS
                     AELRKLLQNNPLNKLSFDASAASKEYQALRQKASQLVNEVIRANMPRMEAELRMKLEQ
                     EVDRMFKEIRKK"
BASE COUNT          463 a          755 c          597 g          330 t
ORIGIN      
        1 cccggcggca aggccagccg ccccggcgac atcgtcacca ccatgagtgg ccagaccgtc
       61 gagatcctca acaccgacgc cgaaggccgt ctggtactgt gcgacgccct cacctacatc
      121 gaacgctatg aacccgcgtc cgtggtggac atcgccaccc tgaccggcgc catcatcgtg
      181 tcgctcggcg cccatgccac cggcatgttc agcaacaacg acggcgtggt ggagcagctc
      241 aagcaggctg ccgactacac ccaggaccgg gtgtggcacc tgccgatctg ggaggactac
      301 cagtcccagc tggacagccc tttcgccgac atggcaaata tcggcggccc gaaagcaggt
      361 tccatcaccg ccgcctgctt cctgtcccgc tacaccaaaa agtatccctg ggcccacctg
      421 gatgtggccg gcacctcctt cctgggccag cccaagggcg ccacaggccg gccggtaccg
      481 ctgctgtccg aatacctgat cagccagacc cgcaagaaat gacgcaggtc gatttctaca
      541 tcctgccgga agacaacagc ctggagccgc tgctgtatgc agcgcgactg atcgagaagg
      601 ccttccggcg cgggcaccgc ctttacgtgc acacggccag cgccgccgac acccaggccc
      661 tgagcgatct gctttggcag cgggacggca gctttctggc ccatggcgcg ctccatgaca
      721 cgtcgtctgc ggcacccggc gaagccattg ttgtgtccca tctgggtaac cctggccagc
      781 acagtgacgt gctggtcaat ctggccggca ccgttccgcc gttttgccag aactttcaaa
      841 ggattgcgga aatcgtgccg ggcaaaccgg aatcccggtc acaaagccgg gagaatttcc
      901 gccactacaa ggaaaagggc tattctttaa agacgcacaa tatccaaccg tgagacttcc
      961 ccatgaacca cggcgacagc aagacttcgc tcaaacaaca agaaaaacaa aaaatgctgg
     1021 atgaactgga atccctcagc cgcatgctgg acgagaatct cgacgacgag gatgacaccc
     1081 caccccgccc ggttccggaa gacattcccg tgctgtaaag ctttgtggac gatgtgccgg
     1141 ttctcaatga gcgcttcctc gatgaggaac ccgagatcat tgcgccgccg ccgaagccca
     1201 aaccagccca gaaaaccgat tccgtggatc agtcggctgc ccgcttcaat acggcaccag
     1261 cccccgattt cagtggcctg ctggatgaca gcttcctgga caatgatcct ctggaaatca
     1321 gcgccgccgt gcgccagcac aaacccgttc cggaaccacc gctgatgcgg gaagcgctgc
     1381 aactggaccc ggaatcggta cgaccggccg caccggtggc gactgccagg ccggcgcaag
     1441 cagaatcccc cgcaccgcag ccacgcgcca gtgcgcccgt tccgccgcct gtcacggcgc
     1501 gcactgcagt gccggaaccg caagcggcac gtccggagcc ggtgcgtccg ccggcagcac
     1561 cagcggccac cacaaaaccc gcagtttcgg ctccgttgcc gtccgccagt caggctgtgt
     1621 ctgctccggc acccgctgcc gcgccggtcc cgcacaccgc cgccagcatt acccagcccc
     1681 tcaacaggtt ccagaccagc agcaagaacg aaaacccgtt cctgcccaag tccaccctcg
     1741 acaagatccg cgagaatcac cagcagtacg acgcctcggc ggagctgcgc aagctgctcc
     1801 agaacaaccc gctgaacaag ctgtcgtttg atgcttcggc ggcctcgaag gagtaccagg
     1861 ccctgcgcca gaaggccagc cagctggtga acgaggtgat ccgcgccaac atgccccgca
     1921 tggaggccga actgcgcatg aagctggagc aggaagtcga ccgcatgttc aaggaaatcc
     1981 gcaaaaagta gccctgcgct gctgccaccc ggcagcaaaa tcccgcccgc accggtataa
     2041 tcggcagcct tcgcgaattc tctggcggag gcgctgagtc cggcattgcg gtttgggaca
     2101 cgccacaagt acatccctgt aggctcgaca gcggccatcc atggc
//
LOCUS       DASUEG010000517         6277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1078977, whole genome shotgun sequence.
ACCESSION   DASUEG010000517 DASUEG010000000
VERSION     DASUEG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078977"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFX11_09715"
     CDS             complement(<1..848)
                     /locus_tag="VFX11_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4938941.1"
                     /translation="MSRVRQGIKRGPVAWMVHNRVTPNLLMLVLLVGGLLMSLQIKKE
                     VFPEFQMDLVTVTVALPGASPEEVEQGVVLAIEESVRGLEGVEEVVSTAAESLATVMI
                     ELQSGVDRQKAYQDIQQAVDRITTFPDDAERPQISLNNRRREVLEVQLHGNVSEQVLR
                     AAAEQVRERLLADRDLTQIDLLGAREMEIHVEIPAATLRAHGLTLGQVAQTIGDSALD
                     RSGGKIETRGGEILLRVQERKDFAREFAAIPLRVNGNGSVLRLGDVATVREGFADTNI
                     SATFNN"
     gene            complement(845..2107)
                     /locus_tag="VFX11_09720"
     CDS             complement(845..2107)
                     /locus_tag="VFX11_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4938942.1"
                     /translation="MNQKLDLEPNQATSLTGTEPAFSGAETPRPLWLRFGLPLLVLVV
                     AVLVTWQLMSSSPKAARAHKEREARLVETIAVTQADTLVPVQAWGEVKPARSITLKAQ
                     VSGIVKTLEAREPGTFFRQGDTLLQLDTTDYELALAQRRSELEQALANLEIEQGNQAV
                     ALSEYELLGTQASAAEKKRMLRGPQLQSANAQVAAARAAVEEARTQLARTTVTAPFEA
                     VLQERHVDVGSHVTISGNLLELVGAEAAWLEVAVPVTQLRWIRFPGAGSMGSTVKIFY
                     DQVWAPQQMRTGRVIRLLSQLEENGRMARVLVQVEDPFARTDANRDQPRLMLGSFVRT
                     EIEGIPVQQAVVIPRALLRENDTVWILKDGNTLEIRAVTVAYRDQENVFIAEGMRAGE
                     QVISSALSTATDGMPVRVKNLPAGERAP"
     gene            complement(2132..3568)
                     /locus_tag="VFX11_09725"
     CDS             complement(2132..3568)
                     /locus_tag="VFX11_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4938943.1"
                     /translation="MMKGLKRPHFFRSFALPLVLTLAVSGCAHQTPDPGPQEQLPERF
                     SRSGDVVLPAQFWQAFADAELDALVQQALQQNHSLRATAETLVQARAVLRQRDAARWP
                     SVDMNLSAQRIRGDSANAGDIYSGGLAASYELDLWGRVSSAADAAEFDLLATEEAIQA
                     ARISLAAEVTSVWYQLVGQRSLQTLQLQQIDANEKVLQLITARFQQGLSDAADVLRQK
                     VLLEQNHAALADYNANIAVLEHALAVLTGAVAGTAQFPDQAILAELPPLPDTGLTADW
                     LQNRPDIRQAFYGLQAADARLAVAVAERYPRIDLTASVTSASTSASSLFDDWFDTLAA
                     NALLPLFDAGARKAEVTRNESLFRQQFERYQQTVLQAVAEVEDALTRESYQQTRIQHL
                     HNKLELNNQVVQRLGLRYRSGNVDYLDVLQALVDKQQTEQDLVTARQQRTGYRIALAR
                     ALATGWVEADSSQGSSQDPSQNQATSPN"
     gene            complement(3729..4547)
                     /gene="eutC"
                     /locus_tag="VFX11_09730"
     CDS             complement(3729..4547)
                     /gene="eutC"
                     /locus_tag="VFX11_09730"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020328691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX4938944.1"
                     /translation="MASDDLPDDNNLIRNDWSVLREFTPARIGLGRAGTSLPTRVNLQ
                     FQLDHARARDAVQAPLDVPALQAQLGAIGLVSQPVKSMAHDRRTYLQRPDLGRTLQSA
                     DAGRLREQWHGKTPWDIAILVADGLSSLAVARHAAPLLQQLMPLLQAQYRVAPVCVAE
                     QGRVALGDDVGEALQAKLVLVLIGERPGLSSPDSLGIYLTWQPRRGRTDADRNCISNV
                     RPQGLDYAAAAQTCAYLIAGAFKAGLSGVQLKDQSRVLENTAINAIPFLTNHQS"
     gene            complement(4537..5937)
                     /locus_tag="VFX11_09735"
     CDS             complement(4537..5937)
                     /locus_tag="VFX11_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX4938945.1"
                     /translation="MNFSTVLGHQTYQFADLKALLAKASPARSGDYLAGIAAATREER
                     VAAQWALADVPLAAFLQNPLIPYEEDEVTRLILDSHDPESFRILNAMTVGDFRNWLLS
                     DAADSNALTAIRKAVTPEMAAAVSKLMRVQDLILVARKCRVTTAFRNTIGLPGCLSTR
                     LQPNHPTDSAAGIAASIVDGLLYGNGDAVIGINPATDNIQQVTRLLHLLDDVISRFAI
                     PTQACVLTHVSNSLLAMEQGAPVDLVFQSIAGSEAANRSFGIHLDMLKEARQAALELK
                     RGTVGNNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARAFDPLLVNTVVGFIGPE
                     YLYDGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDMLLTQFAVAGGTFI
                     MGIPGSDDVMLNYQTTSFHDALYVRQVLGLKPAPEFERWLVRNGIFNAAGGLATGAAL
                     PDSFQKWLRLEQQHGQ"
BASE COUNT         1163 a         2077 c         1865 g         1172 t
ORIGIN      
        1 tggttgttga aggtggcgga aatgttggtg tcggcaaaac cttcgcgcac ggtggcaacg
       61 tcacccaacc gcaagacgct gccattgccg ttcacccgca ggggaatggc ggcaaattcg
      121 cgggcgaaat ccttgcgctc ctgcacccgc agcaggatct cgccgccgcg ggtttcaatc
      181 ttgccgcccg agcgatccag cgcggaatca ccgatggtct gcgccacctg ccccagggtc
      241 aggccatggg cgcgcagggt tgcggccgga atttccacgt gaatttccat ctcgcgcgca
      301 cccagcagat cgatctgggt caggtcgcga tccgccagca accgctcgcg cacctgttcg
      361 gccgcggccc gcagcacctg ctcgctgaca ttgccgtgca actgcacttc cagcacttcg
      421 cggcggcggt tgttgagaga gatctgtggt ctttcagcat cgtcaggaaa ggtggtgatg
      481 cgatccactg cctgctggat atcctgatac gccttctgcc ggtcaacacc ggactgcagc
      541 tcgatcatca ccgtggccag gctttcagcg gcggtggaca ccacctcctc cacgccttcc
      601 agcccgcgca cgctttcctc gatcgccagc accacgccct gctccacttc ctcggggctg
      661 gcaccgggca acgccacggt gaccgtgacc aggtccatct ggaattcggg aaacacttcc
      721 ttcttgatct gcagcgacat cagcaggccg cccaccagca gcaccagcat cagcaggttc
      781 ggcgtgacgc ggttgtgcac catccaggct acgggcccgc gcttgatacc ctgccgaacc
      841 cggctcatgg cgcgcgctcc ccggccggca ggtttttcac gcgcaccggc atgccatcag
      901 tggcagtgga cagggcagac gaaataacct gctcaccggc ccgcatgcct tccgcgatga
      961 acacgttttc ctgatcgcgg taagccacgg tgacggcgcg gatttccagt gtgttgccgt
     1021 ccttcagtat ccagaccgtg tcgttttcgc gcagtagcgc gcggggaatc accactgcct
     1081 gttgcaccgg gataccttcg atttcagtgc gcacgaaaga acccagcatc aggcgcggct
     1141 ggtcacgatt cgcatcagtg cgggcaaagg gatcctccac ctgcaccaga acccgcgcca
     1201 tgcggccgtt ttcctccagc tggctgagca gacggatcac ccggccggtg cgcatctgct
     1261 gcggtgccca cacctggtcg tagaaaattt tcacggtgga acccatgctg ccggcaccag
     1321 gaaaacgaat ccagcgcaac tgcgtcaccg gcactgccac ttccagccac gcggcttcgg
     1381 cgcccaccag ttccagcagg ttgccgctga tggtgacatg gctgccaaca tccacatggc
     1441 gctcctgcaa taccgcctcg aacggcgcgg tcacggtggt gcgggccaac tgggtacggg
     1501 cctcctcaac cgcggcgcgg gctgcagcca cctgggcatt ggccgactgc aactgcggcc
     1561 cgcgcagcat gcgttttttc tccgccgccg aggcctgggt acccagcaac tcgtattcgc
     1621 tgagcgccac cgcctgattg ccctgctcga tctccagatt cgccagcgcc tgttccagtt
     1681 cgctgcgacg ctgggccagg gccagttcgt aatcggtggt atccagctgc agcagcgtgt
     1741 cgccctggcg gaagaaggtg ccaggctccc gcgcttccag cgtctttacg ataccgctta
     1801 cctgcgcctt gagagtgatt gatcgggccg gtttcacttc gccccaggct tgcaccggaa
     1861 ccagggtatc cgcctgggtg acggcgatgg tttccaccag ccgggcctcc cgttccttgt
     1921 gcgcacgggc cgccttgggc gaactggaca tcagctgcca ggtcaccagc accgccacaa
     1981 ccagcaccag cagcggcagc ccaaagcgca gccacagggg ccggggcgtc tccgcaccgg
     2041 agaacgccgg ttcggtgccg gtcagggagg tggcctggtt cggctccaga tcgagcttct
     2101 gattcatcgg gttttcactc attactcaca ttcaattcgg tgacgtggcc tggttttgtg
     2161 aagggtcttg agaagagccc tgcgaagaat ccgcttccac ccagccggtc gccagcgccc
     2221 gcgccagggc aatacggtac cccgtgcgct gctgccgtgc ggttaccaga tcctgctccg
     2281 tctgctgctt gtccaccagg gcctgcagta cgtccagata atccacgttg ccggaccggt
     2341 agcgcaggcc cagacgctgc accacctgat tgttcagttc cagcttgtta tgcagatgct
     2401 gaatgcgggt ctgctggtag gattcccggg tcagggcatc ctccacttcc gccaccgcct
     2461 gcaacaccgt ctgctgatag cgttcgaact gctggcggaa cagggattcg ttgcgggtga
     2521 cttcggcctt gcgggcgccg gcgtcgaaca ggggcaagag ggcgttggcg gccagggtgt
     2581 cgaaccagtc gtcgaacagg ctggaggcag acgtggaagc gctggtgacc gaggcagtca
     2641 gatcgatgcg gggataacgt tccgccaccg cgacagccag ccgcgcatcg gccgcctgca
     2701 ggccataaaa cgcctggcgg atatcggggc ggttctgcag ccagtcggcc gtgagccccg
     2761 tgtccggcag cggcggcaat tccgccagga tggcctggtc cggaaactgc gcggtgccgg
     2821 caacagcacc ggtcagcacc gccagcgcat gttccagcac ggcgatattg gcgttgtaat
     2881 ccgccagggc cgcgtggttc tgctccagca acaccttttg ccgcagcacg tccgccgcat
     2941 cggacaggcc ctgctgaaac cgcgcggtaa tcagctgcaa tactttttcg ttggcgtcga
     3001 tctgctgcag ttgcagtgtc tgcaggctgc gctggcccac cagctgatac cagaccgagg
     3061 tcacttccgc cgccagggaa atgcgggcgg cctgaatcgc ctcttccgtc gccagcagat
     3121 cgaattccgc ggcatccgcc gccgagctca cccggcccca cagatccagt tcgtacgatg
     3181 ccgccagtcc accggaataa atatcgccgg cattggcgga atcgccgcga atgcgctggg
     3241 ccgacaggtt catgtccacg ctgggccagc gcgccgcatc gcgctgccgc agcaccgccc
     3301 gcgcctgcac cagggtttca gcggtggcac gcagggaatg attctgctgc aacgcctgct
     3361 gcaccagggc atccagttcg gcgtccgcga acgcctgcca gaactgcgcc ggcagcacaa
     3421 catcgccgct gcggctgaag cgctcgggca actgctcttg gggaccgggg tcaggcgtct
     3481 ggtgggcgca gccggacacc gccagcgtca ataccagcgg cagggcaaac gacctgaaaa
     3541 aatgcggcct tttcagaccc ttcatcatcc aataaacctt tctgtcaccg gctttgcagg
     3601 acatcacagc atgcaaaggt gcaatcaata tggaaattgt cagaccacga cggggcaggc
     3661 tggttcgcca gccaccggga gcgccccgac tactgcacaa cagtgtaaca ggccgtcgcc
     3721 aggatcactc aggattgatg gttggtcagg aagggaatgg cgttgatggc agtgttttcc
     3781 agcacgcggg actggtcttt cagctgcacg ccgctgagac cggccttgaa ggcacccgca
     3841 atgagataag cacaggtctg cgcggcggca gcgtaatcca gcccctgcgg ccgcacgttg
     3901 gaaatgcagt tgcggtcggc gtccgtgcga ccccggcgcg gctgccaggt aaggtagatg
     3961 cccaggctgt ccggcgaact caggcccggg cgctcgccga tcagcaccag caccagcttc
     4021 gcctgcagcg cttcccccac gtcgtcgccc agcgccaccc ggccctgctc cgccacgcac
     4081 accggcgcta cccggtactg cgcctgcagc aatggcatca gttgttgcaa caggggcgcc
     4141 gcatggcgcg ccaccgccag ggacgacagg ccgtcggcca ccagaatcgc gatatcccag
     4201 ggcgtcttgc cgtgccattg ctcccgcagc cggcctgcgt cggcggattg cagtgtgcgg
     4261 cccaggtccg gccgctgcag ataggtgcgg cggtcgtgcg ccatcgactt caccggctgc
     4321 gacaccagtc ctatcgcacc cagttgcgct tgcagcgctg gcacatccag cggcgcctgc
     4381 acggcatcgc gggcacgggc atgatccaat tgaaactgca ggttcacgcg ggtgggcaac
     4441 gacgtacccg cccgccccag cccgatccgc gccggcgtga attcccgcag cacagaccag
     4501 tcgttgcgga tgagattgtt gtcgtcgggc aggtcgtcac tggccatgtt gttgctccag
     4561 ccgcagccat ttctggaaag aatccggcaa cgccgcgccg gtggccagcc cgcccgccgc
     4621 attgaaaata ccattgcgga ccagccagcg ttcgaattcc ggcgccggtt tcaagcccag
     4681 cacctggcgc acatataagg catcgtggaa tgaggtggtc tggtaattca gcatcacgtc
     4741 gtcgctgccg ggaataccca tgatgaaggt gccacccgcc accgcgaact gggtcagcag
     4801 catgtccatg tcgtcctggt ccgcttcggc gtggttggtg tagcagatat cgcagcccat
     4861 gggcaggccc agcagttttc cgcagaaatg atcttcaaga ccggcgcgaa tgatctgctt
     4921 gccgtcgtac agatattccg gcccgataaa acccaccacc gtgttcacca gcagcggatc
     4981 gaacgcccgc gccaccgcgt aggcccgcgc ctccatggtc tgctggtcca cgccatggtg
     5041 cgcattggcc gacagcgcac tgccctgccc ggtttcaaaa tacatgacgt tgttgcccac
     5101 tgtgccgcgc ttcagttcca gagccgcctg tcgcgcttcc ttcagcatat ccaggtgaat
     5161 gccgaaactg cgattggccg cctcactgcc ggcaatcgac tggaatacca gatccaccgg
     5221 cgcgccctgc tccatcgcca gcaaactgtt gctgacatgc gtcagcacgc aggcctgggt
     5281 tgggatcgca aaacggctga tcacatcgtc gagcagatgc agcaagcggg tgacctgctg
     5341 gatgttgtca gtcgcaggat tgatgccgat cactgcatcg ccgttgccat acagcagtcc
     5401 atcaacgatt gaggcggcga tgccagcggc gctgtcggtg ggatgattcg gttgcaaccg
     5461 ggtggacagg caacccggca gaccgatggt attgcggaac gcggtggtaa cccggcattt
     5521 gcgcgccacc aggatcagat cctgcacccg catcagcttg ctcaccgccg ccgccatttc
     5581 cggcgtgacc gcttttctga ttgcagtcag cgcattgctg tccgccgcat ccgacaacaa
     5641 ccagttgcgg aaatcgccga cggtcatcgc attcagtatt ctgaacgatt ccgggtcatg
     5701 actatccaga atgaggcggg tcacctcatc ctcttcatag ggaatcagcg gattctgcag
     5761 aaacgctgcc agcggcacgt ctgccagcgc ccattgcgcc gctacccgtt cctcgcgggt
     5821 ggccgcggct attcccgcca gataatcgcc ggaacgggcc gggctggcct tcgccagcag
     5881 cgctttcaaa tctgcgaatt ggtaggtctg atgacccaga acggtgctga aattcatgtc
     5941 atgccaacca ttagttcacc ctgatttgaa aagcaaatta tctgccagta attagcctgt
     6001 ttcctggcag tttcagccca gcctatacgg taacaaatag aaacagacaa tcccgcgggg
     6061 ttcgcgctag tgttaaagct ccaaggttac attccggggt gggttctgca tatgagtctg
     6121 cttgtgagtg atagcaagaa agccggcaag gtggatttca aactggccca ccgtcttacc
     6181 cgtcgccatg atctggcgtt cctgcgcttt attaccgaaa ccggcgacca ggattccgtg
     6241 ctgctatccg aacaggcaaa ccagtggctc atgcaac
//
LOCUS       DASUEG010000518         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1613811, whole genome shotgun sequence.
ACCESSION   DASUEG010000518 DASUEG010000000
VERSION     DASUEG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613811"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFX11_09740"
     CDS             <1..768
                     /locus_tag="VFX11_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX4938946.1"
                     /translation="GIAVARHCFAHDAESLRTAAGELGFPLIVKAAEQGYDGRNQWRL
                     ADPSALDRFVGQPVTGDWVVEEQVPFLREISLLAARSAQGEIRFYPPTENRHVNGVLR
                     TSLAPAIDLPEPALQAACEKVRQMLQRTQYVGVLALEYFVLEDRLCLNEVAPRVHNSG
                     HWTQQGSTTSQFENHLRAILGWPLGACDAKGHAGMVNILGTDAPPDALLSEAVTLHLY
                     DKQKRPGRKLGHVNVRDDDRAKVEAELQRLERRLGGG"
     gene            complement(817..1785)
                     /locus_tag="VFX11_09745"
     CDS             complement(817..1785)
                     /locus_tag="VFX11_09745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX4938947.1"
                     /translation="MRALQVEAFGDPKALRVSDLPERSPGSGEIKLRMGGVGIGFFDG
                     LLVKGEYQIKPPLPFVPGSSIAGVVEEVGDGVTHLKVGDHVAGFALLGGLAEKITLNA
                     MSCFAIPKAIPLVDAANFFVAYATGLYGLRECGRLQPGETVLVLGASGTTGSTAIEIA
                     KAMGARVIAAASTEAKRQRCVEAGADVTVDSSSDGWRQQVKSVMGRGVNVVYDPVGGD
                     FTEAALRLMAPGGRHLIVGFVTGIAKIPSNLLLLKRCSIAGVNWGGETMENPAIVPPV
                     IQQLVEWTVAGKLKTRPDHVFPLERTGEAFESIFNRSSSGKIVITP"
     gene            complement(1787..2566)
                     /locus_tag="VFX11_09750"
     CDS             complement(1787..2566)
                     /locus_tag="VFX11_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4938948.1"
                     /translation="MEHVKVSQEGHVLVIYIDRAEKYNALSPAIYHAIGKALKRLNDD
                     PELRVAVLHAAGKHFTAGVELDLWAPIFGSGSAFPVQPDEIDPMGLTGPRHTKPLIIA
                     VQGYCFTWGVEILLNTEIRIAARDTQFQMLEVQRGLYPCGGATLRLPKEAGWGNAHKV
                     LLTGERWSAEEAYRWGMVQELVEPGEQFNKAMEYAQKIADCAPLGVQGLLKSTRTAET
                     EDHATAVNQMFVDLVPVMKSEDAAEGVKSFIERRKAVFKGK"
     gene            complement(2600..>4259)
                     /locus_tag="VFX11_09755"
     CDS             complement(2600..>4259)
                     /locus_tag="VFX11_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4938949.1"
                     /translation="IAERYFAPHNHLADEQEPWFDGEKVHTIPEVKAAFHHFVDSGLL
                     NARQDYEDGGMQLPATIAAACTAQITAANPSTAGYPFLTMAAANLIAHFASVGLQQKY
                     LPLMRSGKATGTMALTEPDVGSSLADIKTAATPTAQGHYLIRGHKMFISGGDHDLTDN
                     IVHLVLARIKGAPAGVKGISLFLVPKWLVDDNGQPQQRNDVALAGLLHKMGYRGTTST
                     VLNFGEKDQCVGYLIGEAHAGLKYMFMMMNEARIGVGLGAAVIGYRGYVESLAYARER
                     PQGRHPGNKDSNSAPIPIIEHADVKRMLLAQKVYAEGALALCLYASTLVDTLENSSDD
                     SERNNARELLDLITPVVKSWPAEFGCKANELAIQVLGGCGYTREYPVEQCYRDNRLNP
                     IHEGTHGIQALDLLGRKLWQNGSAGLKQLGREIMTTIARASAVPALQQETKALQDALQ
                     LVTALTGKLGQQLQTQGPDVALANASVFLSLTGHIVVGWIWLWQATVAHEKMQGDAAN
                     AGFYKGKLQACRYFFRWELPQIQHWNQLLQSEDDTCRTMQNDFF"
BASE COUNT          787 a         1305 c         1297 g          870 t
ORIGIN      
        1 ggcattgcag tcgcccgtca ctgctttgcc catgatgctg aatcgctacg gactgccgcc
       61 ggtgaactgg gtttcccgct gatcgtgaag gcggccgagc agggttacga tggccgcaat
      121 cagtggcgtc tggccgatcc ctccgcgctg gaccgcttcg tcggtcaacc ggtgacgggg
      181 gattgggtgg tcgaagagca ggtgccgttc ctgcgggaaa tatcgctgct ggcggcccgg
      241 tctgcccagg gcgaaatccg cttttatcca cccactgaaa atcggcacgt caatggcgtg
      301 ctcagaacat cgctggcacc ggctatcgat ctgccggagc cggcgctgca ggcagcgtgt
      361 gaaaaagtgc gccagatgtt gcagcggacg cagtatgtgg gtgtgctggc gctggaatat
      421 ttcgtgttgg aggaccggct ctgtctgaat gaagtggctc cccgggtaca caacagcggg
      481 cactggacgc agcagggcag caccaccagc cagtttgaaa accatctgcg tgcaattctg
      541 ggctggccgc tgggcgcgtg tgacgcaaaa gggcacgctg gcatggtcaa tattctggga
      601 accgatgcgc cgccggatgc tttgctgtca gaggcggtca cgcttcactt gtacgacaaa
      661 cagaagcggc ccggtcgcaa actgggccac gtcaatgtgc gtgatgatga tcgtgcgaaa
      721 gtggaagcgg agctgcagag gctggagcgg cgtctggggg gagggtgacg aatcccggcg
      781 cggccggaat tcgtcggtac actcacagac gcgcagttat ggcgtaatca caatcttccc
      841 gctggaactg cggttgaaaa tggattcgaa cgcttcaccg gtgcgctcca gtggaaacac
      901 atggtcgggt ctggttttga gtttgcctgc cacggtccat tccaccagtt gctggatcac
      961 cggcggaacg atggcgggat tttccatggt ttcgccaccc cagttcacgc cggcgatgga
     1021 acagcgtttc aacagcagca ggttcgacgg aattttcgca atcccggtga cgaagcccac
     1081 gatcaggtga cgtccgcccg gtgccatcag ccgcaatgcc gcttcagtga aatcgccgcc
     1141 caccgggtcg tataccacat tcacgccacg ccccatgacg gatttcacct gctggcgcca
     1201 gccgtcgctg gaggaatcca ccgtgacgtc ggcacccgct tccacgcagc gctggcgttt
     1261 ggcttcggtg gacgccgccg caatcacccg cgcgcccatg gccttggcga tttcaatggc
     1321 agtggaaccg gtggtgccgg acgctcccag caccagcacg gtttcgcccg gttgcagccg
     1381 gccgcattcg cgcagaccgt acaggccggt ggcgtaggcg acaaaaaaat tcgcggcatc
     1441 caccagggga attgctttcg gaatcgcaaa gcacgacatg gcattcagcg tgattttctc
     1501 cgccagtccg cccagcaatg caaaaccggc cacatgatcg cccaccttca ggtgcgtcac
     1561 gccgtcgccc acttcttcca ccacgcccgc gatggaactg ccgggcacaa agggcagtgg
     1621 cggcttgatc tggtattcac ctttcaccag caggccgtcg aaaaaaccga tgccgacacc
     1681 gcccatccgc agcttgattt cgccgctgcc gggcgaacgt tcgggcagat cggaaacacg
     1741 gagtgccttg ggatcgccaa aggcttcaac ttgcagtgcg cgcatgttat ttccccttga
     1801 acacagcttt gcgccgttcg ataaaggatt tcacgccttc ggccgcatct tcacttttca
     1861 tcaccggcac cagatccaca aacatctggt tcacggccgt ggcgtgatct tccgtctcgg
     1921 cagtgcgggt ggatttcagc agaccctgca cccccagcgg ggcgcaatcg gcaatcttct
     1981 gcgcgtattc catggccttg ttgaactgct cgcccggttc caccagttcc tgcaccatgc
     2041 cccagcggta ggcttcttcc gcgctccagc gttcgccggt gagcagcact ttatgggcgt
     2101 tgccccagcc ggcttccttc ggcagacgca gggtggcgcc accgcaggga tacaggccgc
     2161 gctgcacttc cagcatctgg aattgcgtat cgcgagcggc gatgcggatt tcggtgttga
     2221 gcaggatttc cacgccccag gtgaagcagt aaccctgcac ggcgatgatc aggggcttgg
     2281 tgtggcgcgg gccggtgagg cccatcggat cgatctcgtc aggctgcacg gggaaggcgg
     2341 aaccgctgcc gaaaatcggc gcccacagat ccagttcgac gccggcggtg aaatgcttgc
     2401 cggctgcgtg cagtacggca acgcgcaatt cgggatcatc gttcagccgc ttcagtgcct
     2461 tgccaatggc gtgatagatc gcgggggaca gggcgttgta tttttcggcg cggtcgatgt
     2521 agatgacaag aacgtggcct tcctgactga ctttgacgtg ttccataaat tcctctgtaa
     2581 aaaaatggcg caatggccgt tagaaaaaat cgttctgcat ggtacggcag gtgtcgtctt
     2641 ctgattgcag caactggttc cagtgctgga tctgcggcag ttcccagcgg aagaaatagc
     2701 ggcaggcctg cagtttgccc ttgtaaaatc cggcattggc agcatcccct tgcatttttt
     2761 cgtgagcgac cgtagcttgc cacagccata tccagcccac aacaatatgg ccggtgaggc
     2821 tgagaaaaac cgatgcattc gccagcgcga catccgggcc ctgtgtttgc aattgctggc
     2881 ccagcttgcc ggtgagcgcg gtgaccagct gcagcgcgtc ctgcagtgct ttggtttcct
     2941 gctgcaatgc cggcacggcg ctggcccggg cgatggttgt catgatttcc cggcccagct
     3001 gtttcaggcc ggcactgccg ttctgccaca atttgcgacc cagcaggtcc agcgcctgga
     3061 tgccgtgggt gccttcgtga atcggattca ggcggttgtc gcgatagcac tgttccaccg
     3121 gatattcccg cgtgtaaccg cagccaccca gcacctggat cgccagttcg ttggccttgc
     3181 agccgaattc cgccggccag gatttgacga cgggcgtgat caggtccagc agttcccgtg
     3241 cgttgttgcg ctcgctgtcg tcgctgctgt tttcaagcgt gtccaccagc gtgctggcat
     3301 acaggcacag tgccagcgcg ccctcggcat agactttctg cgccagcaac atgcgcttca
     3361 cgtcggcgtg ctcgatgatc ggtatcggcg ccgagttcga atccttgttg ccgggatggc
     3421 gcccctgcgg ccgttcccgc gcatacgcca gcgattccac ataaccgcga taaccgataa
     3481 ccgccgcacc cagaccgacg ccgatgcgcg cttcgttcat catcatgaac atgtatttca
     3541 gaccggcgtg ggcctcgcca atcaggtagc caacgcactg atccttctcg ccaaagttca
     3601 gcacggtgga ggtggtgccg cgataaccca tcttgtgcag caggcccgcc agcgccacgt
     3661 cgttgcgctg ttgcggctgg ccgttgtcgt ccaccagcca cttgggcacc agaaacagcg
     3721 agatgccttt cacgcctgcc ggagcccctt tgatgcgcgc cagcaccaga tgcacgatgt
     3781 tgtccgtcag atcgtgatcg ccgccggaga taaacatttt gtggccgcga atcaggtagt
     3841 ggccttgagc ggtgggcgtg gcggcggttt tgatatccgc cagtgacgaa cccacgtccg
     3901 gctcggtcag cgccatggtg ccggtggcct tgccgctgcg catcaagggc agatacttct
     3961 gctgcagccc gacgctggcg aaatgcgcga tcaggtttgc cgccgccatg gtgagaaacg
     4021 gatagccggc ggtggaggga ttcgccgccg tgatctgcgc cgtacaggct gccgcgatgg
     4081 tggcgggcag ctgcatgccg ccgtcctcat aatcctgccg cgcgttgagc aggccggagt
     4141 ccacaaaatg atgaaacgcc gccttcactt ccggaatcgt gtgcactttc tcgccgtcaa
     4201 accagggttc ctgctcgtcc gccagatgat tgtgcggcgc gaaatagcgc tcggcgatc
//
LOCUS       DASUEG010000519         5807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1755502, whole genome shotgun sequence.
ACCESSION   DASUEG010000519 DASUEG010000000
VERSION     DASUEG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755502"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFX11_09760"
     CDS             <1..925
                     /locus_tag="VFX11_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938950.1"
                     /translation="AMLALGLNIVVGYAGLLDLGYIAFYAVGAYLFAILASPHFDLHV
                     SAWMILPVGALLACIAGATLGAPTLRLRGDYLAIVTLGFGEIVRLFMNNLDRPVNITN
                     GPQGINLIDSVSLFGYRLEEGFTLFGTRFSSDYAYYYLFLVLALLTIFICIRLENSRI
                     GRAWVAIREDEIAAQAMGINTRNVKLLAFAMGASFGGVAGGLFASFQGFVSPESFTLM
                     ESVMVVCMVVLGGMGNIAGVILGAVLLTITPEFLRAIINPLQRELFGRMVVDPENLRM
                     LLFGLALIVVMLFRPAGLWPSSRRKEELSHD"
     gene            918..1688
                     /locus_tag="VFX11_09765"
     CDS             918..1688
                     /locus_tag="VFX11_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938951.1"
                     /translation="MTDLLQLKNIEKRFGGLKALNNVSLIIPEGCVYGLIGPNGAGKT
                     TLFNVITGLYQAEAGERVFAGEMLALKTKPHRIVERGIARTFQNIRLFNHMTALENVM
                     VARHGKTRTGVIGAILRHARARAEEQGIRQKAFELLRYVGIESSANKLARELSYGDQR
                     RLEIARALATEPRLLALDEPAAGMNPTETAGLRELIARIKADGITVLLIEHDVKLIMG
                     LCDRVAVLDFGELIAEGVPAEVQSHPRVIEAYLGTGAA"
     gene            1685..2395
                     /locus_tag="VFX11_09770"
     CDS             1685..2395
                     /locus_tag="VFX11_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4938952.1"
                     /translation="MSLLAINNLSVAYGGIKAVKGISLNVEEGELVTLIGANGAGKTT
                     TLNTLGGLLTPSGGSLQYAGHDLLKLPAHVRVQHGMALVPEGRGVFARLTVAENLLMG
                     AYHRNDKADIAADLQKSYALFPRLKEREHQLAGTLSGGEQQMLAIGRALMSRPRLLLL
                     DEPSMGLAPLMVEKIFEIIRSINAQGVAILLIEQNARLALAAAHRAYVMESGAITLSG
                     SAAELAADERVKAAYLGH"
     gene            2435..3352
                     /gene="rfbD"
                     /locus_tag="VFX11_09775"
     CDS             2435..3352
                     /gene="rfbD"
                     /locus_tag="VFX11_09775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX4938953.1"
                     /translation="MKILLFGKSGQVGWELQRSLAPLGELVALDRHGDIHLCGDLSDP
                     DGLVRTIRSVAPDVIVNAAAYTAVDNAEHEPGHVQTINALAPGLMAREACAIDAWLVH
                     YSTDYVFDGSGSRPWREDDATAPLNVYGKTKREGEEAIRDSGCKHLVFRTSWVYGTRG
                     RNFPRNLLARAAEQEVLSVVDDQIGAPASAELIADVTAHALRMAMQSPALGGLYHLTA
                     SGEVSWCDYAHFLMAEARRMGMVLKAKTIEPVTTIERPVVARRPLNSRLDCSRIQQSF
                     NLYLPPWQQGVSRWLEESRLIAQEPSSCR"
     gene            3343..3894
                     /gene="rfbC"
                     /locus_tag="VFX11_09780"
     CDS             3343..3894
                     /gene="rfbC"
                     /locus_tag="VFX11_09780"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX4938954.1"
                     /translation="MQVIATALPDVLLLKPRVFADERGYFMETWNQRVYAEHGLDLHF
                     VQDNHSRSHRHTLRGLHYQVVQPQGKLVSVSRGEVYDVVVDVRKNSPTFGQWAAEILS
                     EDNHHLLWIPPGFAHGFYVLSEVADIHYKCTDFYAPEHERCIRWDDPALAISWPIEDG
                     VAPRLSVRDGQGVFLSVAEPVAC"
     gene            4031..5179
                     /gene="traF"
                     /locus_tag="VFX11_09785"
     CDS             4031..5179
                     /gene="traF"
                     /locus_tag="VFX11_09785"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HEX4938955.1"
                     /translation="MRKLPLAVAIAVLGATPALSLADIFDARSMGRGGVGLTMGEYNQ
                     AILNPALINHFDEDDEFSFALNAGVFASDKDDMLQATEDAQDAIDALEENAAPSLADV
                     NDANEQLKNLSGRVVQADVGTSLLIGIPNKSLPAAVVVRGKLSFGAVVDYDENDALVL
                     AAIDAGLATQDDLQSSITASAVAVTEGGFMFGHKANGLELGATLKAQQVELISYTANV
                     ANFDEGDIADGDLSEKSSSVNVDLGASLRLGEKGEFVVAGTVENLVPQSFDGPLDTEY
                     EMEPVVTAAVGYGNTYFKAEANMDLTTRNGYDLLADTQFVRVGVEFSAGRHMHLRAGY
                     RSDLEDNVSDVTTLGLGITPFDRFNIDLSGMIGQGETLGAALQIGFKI"
     gene            complement(5246..>5807)
                     /locus_tag="VFX11_09790"
     CDS             complement(5246..>5807)
                     /locus_tag="VFX11_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4938956.1"
                     /translation="DTYPGVEVQLAVENRETIIQRLDKGLDDLTVMMLPPADGRLRTL
                     PFMESPLVVVAAKGHRLEGLPLTLSSLQDERWLLREVGSGGRQMVEAHFSREKFSPRI
                     AMALGSNEAIKHAVAGGLGITVLSRHALGRDPASLGLVELDVKGFPLPGEFCFVMREG
                     RRLSPAAEAFVQFARHQHHASDQPGK"
BASE COUNT         1079 a         1663 c         1748 g         1317 t
ORIGIN      
        1 cgcgatgctt gccttgggcc tcaatatcgt tgtcggctat gccggcctgc ttgatctggg
       61 ctacatcgca ttctatgccg tgggtgcgta tctctttgcc atactggcct cgccgcattt
      121 cgacctgcat gtctctgcat ggatgatcct gccggtaggt gctctgctcg catgtattgc
      181 cggggcgaca ttgggcgcac ctacgctgcg tctgcgcggg gattacctgg ccatcgtcac
      241 gctcggtttt ggcgaaatcg tccgcctgtt catgaacaat ctcgaccgtc cggtgaatat
      301 caccaacggc ccgcagggca tcaacctcat cgattcggtc agcctgttcg gctaccggct
      361 ggaggaaggg ttcacactgt ttggtacacg cttcagcagt gactacgcct attactacct
      421 gttcctcgtg ttggcgctgc ttaccatttt catctgcatt cgcctggaaa actcccgtat
      481 cggtcgtgcc tgggtggcca ttcgcgaaga cgagatcgcc gcgcaggcca tgggtatcaa
      541 tacccgtaac gtgaagctgc tggcttttgc catgggcgcc agttttgggg gcgtggccgg
      601 cggtctgttt gcgtcgttcc agggctttgt cagccccgag tccttcacgc tgatggaatc
      661 ggtgatggtc gtctgcatgg tggtgctcgg gggtatgggc aatattgccg gcgtcatcct
      721 gggcgccgta ctgctcacca tcacgccgga attcctgcgc gccatcatca atccgctgca
      781 gcgtgagctg ttcggccgca tggtggtcga tccggaaaac ctgcgcatgc tgctgttcgg
      841 actggcgctg atcgtggtca tgctgttccg cccggctggt ttgtggccat cctctcggcg
      901 caaggaggaa ctcagtcatg actgacctcc tccaactgaa aaacatcgag aagcgtttcg
      961 gtggcctcaa ggcgctcaac aacgtcagcc tcattatccc cgaaggctgc gtttacggat
     1021 taatcggccc caatggggcc ggcaagacca cgctgttcaa cgtgatcacc gggctgtacc
     1081 aggccgaagc cggcgagcgc gtatttgcgg gcgagatgct tgcgctgaaa accaagccgc
     1141 accgcattgt cgagcgaggc attgcccgaa ccttccagaa catccgcctg ttcaatcaca
     1201 tgaccgcgct agaaaacgtg atggtggccc gtcacggcaa aacccgtact ggtgtcatcg
     1261 gcgccatcct gcggcatgcg cgggcgcggg ccgaagagca gggcattcgc cagaaggctt
     1321 ttgaattgct gcgctatgtc ggcatcgagt cctcggccaa caaactggcg cgagaactct
     1381 cctatggcga ccagcgtcgc ctggaaattg cccgcgccct ggccaccgag ccgcgcctgc
     1441 tggcgctgga cgaaccggcc gccggcatga accccacgga aaccgccggc ctgcgtgaac
     1501 tgattgcgcg catcaaggcc gacggcatta ccgtgctgct gatcgagcac gacgtgaaac
     1561 tcatcatggg tctgtgtgat cgcgtggccg tccttgattt tggcgaactg attgccgaag
     1621 gcgtgccggc cgaggtgcag tcgcatccgc gcgtgatcga agcctatctc ggaacgggtg
     1681 ccgcatgagt ctgctcgcca tcaataacct ctccgttgcc tatggcggca tcaaggccgt
     1741 gaaaggcatc agcctgaacg tggaagaggg cgagttggtc acgctgatcg gtgccaacgg
     1801 tgccggcaag accaccacgc tcaataccct gggtggcttg ctcaccccct caggcggatc
     1861 attgcagtac gctggtcacg acctgctcaa gttgccagcc cacgtgcgcg tgcagcatgg
     1921 catggcgctg gtaccggaag gacgcggtgt gtttgcacgc cttaccgtgg ctgagaatct
     1981 gctgatgggc gcctaccatc gcaatgacaa ggcggacatc gcggccgacc tgcagaaatc
     2041 ctatgcgctc tttcctcgcc tgaaagaacg cgaacaccag ttggccggta ccctgtccgg
     2101 tggcgaacag cagatgctgg ccataggccg cgccctcatg agtcgtccgc gcctgttgct
     2161 gctggatgag ccatccatgg gactggcccc gctgatggtg gagaagattt tcgagatcat
     2221 tcgctccatc aatgcacagg gcgtggctat ccttctgatt gagcagaacg cgcgactggc
     2281 actggcggca gcacaccgtg cctatgtcat ggagtccggc gccatcacgc tgagtggttc
     2341 tgctgccgag ctggcagccg acgagcgtgt caaggcagct tatctcggac actgaccact
     2401 gatgtgccgt ccggccgtca gtagagggtt gcccatgaag atcctgttat tcggcaagag
     2461 cggacaagtc ggctgggagt tacagcgatc gctggcgccg cttggcgagc tggttgccct
     2521 tgatcgccac ggtgacatcc atttgtgtgg cgatctctct gatcccgacg gactggtgcg
     2581 caccattcgc tccgtggctc ctgatgtgat cgtcaatgcg gcagcctata ccgctgttga
     2641 taatgctgag cacgaacccg ggcatgtaca gaccatcaac gcacttgccc ccggtcttat
     2701 ggcacgcgag gcgtgcgcta ttgatgcatg gttggtgcat tactccaccg attatgtgtt
     2761 cgatggcagt ggctcccgac cgtggcgtga agacgatgca acagcgccgc tcaacgtgta
     2821 tggcaaaacc aagcgtgaag gtgaagaagc catccgggac agcggctgca agcacctcgt
     2881 tttccgcacc agctgggtgt acggcacacg tggccggaac tttccccgca acctgctggc
     2941 gcgtgctgcc gagcaggagg tactgtcagt ggtggatgac cagatcggtg cgccggcaag
     3001 tgccgagctc attgccgatg tcacggccca tgcgctgcgt atggccatgc agtcgccagc
     3061 acttggcggc ctttaccatc tgactgcatc aggcgaggtg tcctggtgtg actacgccca
     3121 tttcctgatg gctgaggcgc gccgcatggg gatggtgctc aaggcaaaaa ccatagaacc
     3181 ggtaaccacg attgagaggc cggttgtggc gcgacgtccg cttaattcgc gtctggattg
     3241 ctcccgcatt cagcagtcct tcaatctgta tctgccacct tggcagcagg gagtatcgcg
     3301 ctggctggaa gagagtcgtc tgatcgcaca ggagccgtcg tcgtgcaggt aatcgcaacc
     3361 gctctgcctg atgtcctgtt actgaaaccg cgcgtgtttg ctgatgagcg cgggtatttc
     3421 atggagacgt ggaatcagcg tgtttatgcc gagcatggtc ttgacctcca ctttgttcag
     3481 gataaccaca gccgttccca tcgtcacacc ttgcgcggtt tgcattatca ggtggttcag
     3541 ccgcaaggaa agttggtcag cgtcagtcgt ggcgaggtct atgacgtggt agtggatgtg
     3601 aggaaaaatt caccgacctt tggtcagtgg gctgctgaaa tcctctcgga agacaatcat
     3661 catctactgt ggattccacc tggattcgcc cacggtttct atgtcctgtc ggaggtggcc
     3721 gatattcact acaagtgcac cgacttctat gcgccggagc acgaacgctg tatccgttgg
     3781 gatgacccgg cactggccat ttcctggcca attgaggatg gcgttgcccc caggttgtct
     3841 gttcgggacg gccaaggggt attcctgtcg gtggcagagc cggtggcctg ttgacagttc
     3901 gtctgtgacg agcggctgtc cattccccca tttgagggtt ttttttctgt tatcccggtg
     3961 ttataactgc cggtcagtgc cccgtcagag ggcatggatt ttccccctgt tactcaacaa
     4021 ggatgctgtc atgcgcaaac tgccgcttgc tgttgctatt gctgttctcg gtgcaacccc
     4081 tgcactttcc cttgccgata tttttgatgc ccgcagcatg gggcgtggtg gcgttggcct
     4141 gacgatgggc gagtacaacc aggccatcct caatccggcc ttgatcaacc attttgatga
     4201 ggatgacgag ttcagttttg cgctgaatgc aggggtgttt gcgtcagaca aggatgacat
     4261 gctccaggca acggaggatg cgcaggatgc aattgatgcc ctggaggaaa acgcggctcc
     4321 atcgcttgcg gatgttaatg atgccaatga gcagctgaag aatctcagtg gcagggtggt
     4381 gcaggccgat gtagggacat cgttgctcat cggtattccc aacaagtccc tgccagctgc
     4441 tgtggttgtg cgcggaaaat tgtcgtttgg tgcagtagtt gattacgacg agaatgatgc
     4501 gctggtgctc gctgcgattg atgccggtct ggcgacacag gatgatttgc agtcgtccat
     4561 tactgcctct gctgttgccg tgactgaagg cggtttcatg tttggtcaca aggcaaatgg
     4621 actggagttg ggtgctactc ttaaggctca acaggtcgaa ctgatttcct ataccgccaa
     4681 tgtggccaat ttcgatgagg gcgacattgc ggatggtgat ctgtccgaga agtcttcgtc
     4741 cgtcaatgtg gatcttggtg ccagcctgcg cttgggcgag aagggtgagt ttgtcgtggc
     4801 gggtacggtt gaaaacctgg tgccgcagtc ctttgatggg cctctcgata ccgaatacga
     4861 aatggagcct gtcgttaccg ctgcggtcgg ctatggcaac acctacttca aagccgaagc
     4921 caacatggat ctgactaccc gcaacggtta tgacctgctg gcagacaccc agtttgtccg
     4981 tgtcggtgtc gagttttcgg ccggtcgtca catgcacctg cgcgcgggtt atcgttcgga
     5041 ccttgaggac aatgtttccg atgtcactac gctgggtctg ggcatcacgc cctttgaccg
     5101 tttcaacatc gacctgtccg gcatgatcgg tcagggcgaa acgctgggtg cggccctcca
     5161 gatcggcttc aagatctgat ccatcaaggt tcgggaagcc cgaatctgcc ctgcgtcatg
     5221 agaagggtcc ctcgtggacc ctttttcatt tgccgggctg atcggaagcg tggtgctgat
     5281 gcctggcgaa ttgaacgaat gcctcggcag ccggtgacag gcgtcggccc tcccgcatca
     5341 caaagcagaa ctcgcccggc agtggaaacc ccttcacatc cagctccacc agccccaggc
     5401 tggctgggtc tcgcccgagt gcatgtcggg atagcaccgt aattcccaga ccgccggcga
     5461 cggcatgctt gatggcttcg ttgctaccca gcgccatggc aatgcggggc gagaacttct
     5521 cccgggaaaa gtgcgcctct accatctggc ggccacccga tccaacctcg cggagcagcc
     5581 agcgctcatc ctgcaggctg gacagtgtta gcggtagccc ttccagtcgg tggcctttcg
     5641 cggccaccac cactagtgga ctttccatga agggcagagt gcgcagtcgg ccatctgccg
     5701 gggggagcat catgactgta aggtcatcaa ggcccttgtc cagtcgctgg atgatggtct
     5761 ctcggttttc gacagccagc tgaacctcca cgccagggta ggtgtcg
//
LOCUS       DASUEG010000520         5621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_793610, whole genome shotgun sequence.
ACCESSION   DASUEG010000520 DASUEG010000000
VERSION     DASUEG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793610"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /gene="dapD"
                     /locus_tag="VFX11_09795"
     CDS             complement(<1..236)
                     /gene="dapD"
                     /locus_tag="VFX11_09795"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016707646.1"
                     /note="catalyzes the formation of
                     N-succinyl-2-amino-6-ketopimelate from succinyl-CoA and
                     tetrahydrodipicolinate in the lysine biosynthetic pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX4938957.1"
                     /translation="MSELQTIIETAFDNRNNISPTSVDAKVKGAVLEALDLLDSGKAR
                     VAEKINGEWVVNQWLKKAVLLSFRIADNAVMDGG"
     gene            complement(488..1684)
                     /gene="dapC"
                     /locus_tag="VFX11_09800"
     CDS             complement(488..1684)
                     /gene="dapC"
                     /locus_tag="VFX11_09800"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX4938958.1"
                     /translation="MNQELELLHPYPFEKLAALFKGTEHRGGLPHIALSIGEPKHASP
                     AFVKQALVDNLDKMSVYPATKGVPELRQAISRWACNRFDLGDGNLCAETQILPVNGTR
                     EALFAFAQVIINRADKPLLVSPNPFYQIYEGAAYLSGAEPYFLPCLEENRFIPDFDAV
                     PTEVWRRCQMIFICSPGNPTGAVMDSATLQKLIALADEYDFVIASDECYSELYFDEQN
                     PPPGLLQVCRQIGRNDYKRCVVFHSLSKRSNLPGLRSGFVAGDAAILKPFLLYRTYHG
                     CAMPVQHQLASVAAWNDEEHVRENRRIYQQKFTAVLDILGDVLDVKKPDASFYLWPRT
                     PIAETEFARRLFEQQHVTVLPGQYLSRTVNGVNPGEYRVRMALVAPLEQCVEAAERIR
                     RFVRSL"
     gene            complement(1690..4386)
                     /locus_tag="VFX11_09805"
     CDS             complement(1690..4386)
                     /locus_tag="VFX11_09805"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX4938959.1"
                     /translation="MKTPLVTELKQDPELFDPERFAQWVQELGSVPKAFRHAMKEADT
                     VLNRRFRNMENIKLIIRRRAWTMDQLLQQLWNQLACAKASDIALMAVGGYGRAELHPH
                     SDIDLLILLQDSGNAEQYRADLELFVTQLWDIGLEVGHSVRTIEECTNLARDDITIAT
                     NLMESRTLCGNPELGVRMQKATAPDKIWNVVDFFSAKWTEQIARHNKHNNTEYNLEPD
                     IKNAPGGLRDIQTIGWVTKRYFNANRLSDLVNHGFLTAEEYDMLNAGQSFLWEVRYAL
                     HILTKRSENRLLFDHQRAVAEILGYRDSNANLAVEKFMKRYYRVALSVSQLNDMLLQH
                     FDEAILRAGQPDTITPINKRFQIRNGFIETTHEKVFEYTPSALLEIFLILAQNEQIEG
                     IRAATIRQIRDHRHLVDDKFRNDIRNISFFMELLRSPNKIYTQLYRMKRYGILGNYIP
                     SFGQVIGQMQYDLFHIYTVDAHSLHVLRNMRMFRHSEVKKDFPIVNMIFHRLPKIELL
                     YIAGLFHDLAKGRGGDHSDLGSVDAYNFCIHHRLSKWDASQVSWLVQHHLLMSLTAQK
                     KDVSDPDVIHEFATKVGDQVRLDYLFALTTADIMATNPNLWNGWRASLLRQLYTETRR
                     ALRRGLENPLNRQDWIEETREKALALLLNRGIAREKVVALWEHLGDDYFLRETFADVA
                     RHTEAILNHPENGGPLVLLGKADDRNYQGAAEVFVYTQDAPNLFAATVAALSQLNLTI
                     ADAKIITSTSNFSLDTYIIMEENGSHIGDDPARAQQIVKKLQKALVDPKKYPEIVHKR
                     MPRALKHFRIPTEVVISNDIAHKRTVLELVALDRPGLLAEIGRIFLDNQVLLQNARIA
                     TLGERVEDVFYITDMTGDMIKDPALCARLENSIKEKLDKPAD"
     gene            complement(4400..5185)
                     /gene="map"
                     /locus_tag="VFX11_09810"
     CDS             complement(4400..5185)
                     /gene="map"
                     /locus_tag="VFX11_09810"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX4938960.1"
                     /translation="MTIIIKTPELIEKMRIAGRLAAEVLEMIGEHVKPGVTTEELDRV
                     CHDYIVNVQQAIPSPLNYRGYPKSICTSVNHVVCHGIPSDKKVLKRGDIVNIDITVYK
                     NGVHGDTSKMFVVGEASIQAERLIRVTQECLYLGIKMVKPGVRLGDIGHAIQQHAENN
                     HFSVVREYCGHGIGEGFHEEPQVLHYGRPNTAVMLEPGMIFTIEPMINAGKRFTKLLP
                     DEWTVVTKDHKLSAQWEHTILVTDTGFDVLTLRKEEAGIFGPA"
     gene            5506..>5621
                     /locus_tag="VFX11_09815"
     CDS             5506..>5621
                     /locus_tag="VFX11_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX4938961.1"
                     /translation="MAQVTMRDMLKAGVHFGHQTRYWNPKMNQYIFGSRNKI"
BASE COUNT         1047 a         1632 c         1660 g         1282 t
ORIGIN      
        1 tagccgccgt ccatgacggc gttgtcggca atccggaacg acagcagcac ggcctttttc
       61 agccactggt tcacgaccca ctcgccgttg attttttccg ccacgcgggc tttgccgctg
      121 tccagcaggt cgagcgcttc cagtacggcg cctttgactt tcgcgtccac cgaggtcggc
      181 gagatgttgt tacggttatc gaaggcggtt tcgatgatgg tctgtaattc cgacattgcg
      241 gttgctccag ggtttgcttt aatacttttg cgatttgaga tgggtgttct tttccggtgc
      301 tgtggcgctg gtccgggcaa cgctattccg gacacgctac aagcacatcc atgtgcgctc
      361 gtccgcggcc atccatggcc gcaaacggtc cggaatagcg ttgcccggac cagctttcaa
      421 ttctaacatc tgcgctctta tccaaattta tgatccggcg ctactgactc gtcaaacaat
      481 ttcatttcta caacgaccgc acaaaacgcc gaatcctttc cgccgcttcc acgcactgct
      541 ccagcggcgc caccagcgcc atgcgcaccc ggtattcgcc cggattgacg ccgttcaccg
      601 tgcgcgacag gtattgcccc ggcagcaccg tcacatgctg ctgttcgaac agcctgcggg
      661 caaattccgt ttctgcaatc ggcgtgcgcg gccacaggta gaaactggcg tctggctttt
      721 tgacatccag cacgtcaccc agaatatcca gcacggcggt gaatttctgc tgataaatcc
      781 ggcggttttc gcgcacgtgt tcttcatcat tccatgctgc cacactggcc agctggtgct
      841 gcaccggcat cgcgcagcca tgataggtgc gatatagcaa aaagggcttc agaatcgccg
      901 catcgcccgc cacgaaaccg gagcgcagcc ccggcaggtt ggagcgcttc gacaggctgt
      961 gaaacaccac gcagcgtttg taatcattgc gtccgatctg gcggcatacc tgcagcagtc
     1021 ccggtggcgg attctgctcg tcgaaataga gttccgaata acactcgtcg gacgcaatca
     1081 caaaatcata ttcatccgcc agcgcgatca gtttctgcag ggtggcgctg tccatcaccg
     1141 cgccggtggg attgccaggg ctgcagatga aaatcatctg gcagcggcgc cacacttcgg
     1201 tgggcacggc atcgaaatcg gggatgaacc ggttctcttc cagacacggc aaaaaatacg
     1261 gttcggcgcc ggacagatac gccgctcctt cataaatctg gtagaacgga ttgggtgaca
     1321 ccagcagcgg cttgtcggcg cgattgatga tcacctgtgc aaacgcaaac agcgcttccc
     1381 gcgtgccatt taccggcagg atctgggttt ccgcgcacag attgccgtcg cccagatcaa
     1441 accggttgca ggcccagcgg ctgatggcct gacgcaattc gggtacgcct ttggtggcag
     1501 ggtacaccga cattttatcc agattgtcca ccagcgcctg tttcacaaac gccggactgg
     1561 catgtttggg ctcgccgatc gacagcgcga tgtgcggcag gccgccgcgg tgctccgtgc
     1621 ccttgaacag cgcggcaagt ttttcaaacg ggtagggatg cagcagttcc agttcctggt
     1681 tcatcgtgtt cagtcagccg gtttgtccag tttctctttg atggaattct ccaggcgcgc
     1741 gcacagcgcc gggtctttga tcatgtcgcc ggtcatgtcg gtgatgtaga aaacgtcctc
     1801 tacgcgttcg ccgagcgtgg cgatgcgggc gttttgcagc agcacctgat tgtccaggaa
     1861 gatgcggccg atttccgcca gcagaccggg ccggtccagc gccaccagtt ccagcaccgt
     1921 gcgcttgtgg gcgatgtcgt tgctgatcac cacctcggtg ggaatgcgga aatgcttgag
     1981 tgcgcgcggc atgcgcttgt gcacgatctc ggggtatttt ttcgggtcga ccagtgcctt
     2041 ttgcagcttc ttcacgatct gttgcgcgcg ggcaggatcg tcgccgatgt gcgatccgtt
     2101 ttcctccatg atgatgtagg tgtccagact gaaattgctg gtggaggtaa tgatcttcgc
     2161 gtcggcgatg gtcaggttga gctggctcag cgcggccacc gttgccgcaa acaggttggg
     2221 cgcatcctgc gtgtacacga atacttccgc cgcgccctgg taattgcggt cgtcggcctt
     2281 gcccagcagc accagcggac cgccgttttc cggatgattc agaatggctt ccgtatgccg
     2341 cgccacatcg gcaaaggttt cccgcaggaa ataatcgtcg cccaggtgct cccacagcgc
     2401 caccactttt tcccgcgcaa tgccccggtt cagcagcaat gccagtgcct tttcccgggt
     2461 ttcctcgatc cagtcctgcc ggttgagcgg attttccagg ccgcgccgca atgcgcgccg
     2521 ggtctcggtg tacagctggc gcagcagtga cgcccgccag ccgttccaca gattgggatt
     2581 ggtggccatg atgtcggcgg tggtgagggc gaacagatag tccagtcgca cctggtcgcc
     2641 taccttggtg gcaaattcat gaatcacgtc aggatcggac acatcctttt tctgcgccgt
     2701 cagtgacatc agcagatggt gctgcaccag ccacgacacc tggctggcat cccacttgct
     2761 cagccggtgg tgaatgcaga agttgtatgc gtccaccgag cccagatcgg aatggtcacc
     2821 gccacgtccc ttggccagat cgtgaaacag gccggcgatg tacagcagtt caatcttcgg
     2881 caggcggtgg aaaatcatgt tgacgatcgg aaaatctttc ttcacttccg aatgacggaa
     2941 catacgcatg ttgcgcagta catgcagcga gtgcgcgtcc accgtgtaga tgtggaacag
     3001 gtcgtactgc atctggccga taacctggcc aaacgacgga atatagttgc ccagaatgcc
     3061 gtagcgcttc atgcggtaca gctgggtgta aatcttgttg ggcgagcgca gcagttccat
     3121 gaagaaggaa atattgcgga tgtcgttgcg gaacttgtca tccaccagat ggcggtgatc
     3181 gcggatctgg cggatggtgg cggcgcggat gccttcaatc tgctcgttct gcgccaggat
     3241 cagaaaaatc tccagcaacg ccgatggcgt gtattcgaac actttctcat gcgtggtttc
     3301 gatgaagccg ttgcggatct ggaagcgctt gttgatcggg gtgatcgtgt ccggctgacc
     3361 agcccgcagg atcgcttcgt cgaagtgctg cagcagcatg tcgttcagct gcgacaccga
     3421 cagcgccacc cggtaatagc gcttcatgaa tttttccact gccaggttgg cgttgctgtc
     3481 gcgatagccc agtatttccg ccacggcgcg ctggtggtcg aacagcaggc ggttttcgct
     3541 gcgtttggtg aggatgtgca gggcgtaacg tacttcccac aggaaggact ggcccgcatt
     3601 cagcatgtcg tactcttctg cggtcagaaa accgtggttg accagatcgc tgagccggtt
     3661 ggcgttgaaa tagcgcttgg tcacccagcc gatggtctgg atgtcgcgca ggccgccggg
     3721 cgcgttcttg atgtccggct ccagattgta ttccgtgttg ttgtgcttgt tgtggcgcgc
     3781 tatctgttcg gtccacttgg cgctgaagaa atccaccaca ttccagatct tgtctggcgc
     3841 cgtggccttc tgcatccgga cacccagctc gggattgccg cacagggtgc gcgattccat
     3901 caggttggtg gcgatggtga tgtcgtcgcg ggccaggttt gtgcactcct cgatggtgcg
     3961 cacactgtgg cccacttcca gcccgatgtc ccacagctgg gtgacgaaca gttccagatc
     4021 agcgcggtat tgctcggcgt tgccgctgtc ctgcagcagg atcagcaggt cgatatcgga
     4081 atgggggtgc agttcggcgc ggccataacc gcccaccgcc atcagtgcaa tgtcactggc
     4141 cttggcgcac gccagctggt tccacagctg ctgcaacagc tggtccatgg tccaggcgcg
     4201 gcggcggatg atcagcttga tgttttccat gttgcggaag cggcggttca acaccgtatc
     4261 cgcttctttc atggcgtgac ggaacgcctt cggcaccgaa cccagttcct gtacccattg
     4321 cgcgaagcgc tcggggtcga acagttcggg gtcctgtttc agttccgtga cgagaggggt
     4381 tttcatgccg gaatcccgtt caagccgggc caaagatgcc ggcttcttcc ttgcgcaggg
     4441 tcagcacgtc aaagccggtg tcggtcacca ggatggtgtg ctcccactgg gccgacagct
     4501 tgtgatcctt ggtgaccacc gtccattcgt ccggcagcag cttggtgaag cgcttgccgg
     4561 cgttgatcat gggttcgatg gtgaaaatca tgccgggctc cagcatcacg gcggtattgg
     4621 gacggccgta gtgcagtacc tggggttcct catggaagcc ctcgccgatg ccgtggccgc
     4681 agtactcgcg caccaccgag aaatggttgt tctcggcatg ctgctggatg gcgtggccga
     4741 tatcccccag ccgcacgccg ggtttcacca tcttgatccc cagatatagg cactcctgcg
     4801 ttacccggat caaccgttcc gcctggatcg aggcttctcc caccacgaac atcttgctgg
     4861 tatcgccgtg caccccgttc ttgtacaccg taatgtcgat gttcacgatg tccccccgct
     4921 ttaatacctt cttgtcgctg ggaatgccgt ggcagaccac atgattgacc gaggtacaga
     4981 tggacttggg atagccgcga tagttgaggg gcgagggaat ggcctgctgc acgttcacta
     5041 tatagtcgtg gcagacgcgg tccagttcct cggtagtgac gccgggtttg acatgttcgc
     5101 caatcatttc aaggacttct gccgccagtc gtcccgcaat gcgcattttt tcaatcagtt
     5161 ccggcgtctt gatgatgatg gtcatgtagt gcgatttcta tcccggtgat gtcagttgtg
     5221 gtggtgcccg tgcggcccgc gcgcgccggc acgcaaggcc ggggcggaca aaggggctca
     5281 ttgtacatgg cgggtcgcgg cggatatacc cctgaaccgg gagggaattt ccccgtgggt
     5341 gccggtcatc cgggctgcgc gcggttgttg cccgggtgtg gatgtggtat aaagcgccgc
     5401 gcaaattaac cataaaccac acatatgccg acacatggtt cagggtgccc ctcggggttg
     5461 gatcatgggg tatatggagg cctaacccac tctcaggaga agaaaatggc tcaagtcacc
     5521 atgcgtgata tgttgaaggc tggcgtccac tttggtcacc agacccgcta ctggaacccc
     5581 aagatgaacc agtacatctt cggttcccgc aacaagatcc a
//
LOCUS       DASUEG010000521         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1509538, whole genome shotgun sequence.
ACCESSION   DASUEG010000521 DASUEG010000000
VERSION     DASUEG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509538"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFX11_09820"
     CDS             complement(<1..285)
                     /locus_tag="VFX11_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX4938962.1"
                     /translation="MNIVHFTRSTLIWLACLYALLLAPLASASLLWQIEKEGKQNHIF
                     GTIHVADRDIVKLPAHVDRVVSQADVLLLEVKQGAQSQQVIADRTTLQEST"
     gene            complement(319..2010)
                     /locus_tag="VFX11_09825"
     CDS             complement(319..2010)
                     /locus_tag="VFX11_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4938963.1"
                     /translation="MKSRPSGAANTGIPLPLERFYFWEKNQPAKICFTQPMGNGVIKD
                     MTWAEAGRQVRSMAAYLKSIGVKPGDRIAILSKNCAWWILSDLAIWMAGGVSVPLYPT
                     LTADSVRQILQHSESKILFVGKLDGWDSMKSGVDSAIRCVSYPLSPPTDFPTWDDLVA
                     QHAPLTENPSRSHGELATIIYTSGTTGVPKGVMHTFNNIAAGASLGSDIYEIASRDRL
                     LSYLPLSHVAERACIEMMQIYQGTHVHFAESLDTFAQDLQRARPTLFFAVPRIWSKFQ
                     AGVFAKFPEKKLRLLFRVPGLSGYIKKKIRHGLGLDQARLCLSGAAPISNSLLAWYKE
                     LGIEIIEVYGMTENFGYSHTTRLGRGRPGYVGQPNPSVDVKLGDGGEVLVRAPTTMVG
                     YYKEPEKTAEALDADGFLRTGDVGAIEADGSLRITGRIKEIFKTSKGKYVAPFPIEAK
                     LLADSHVEQCCVVGNAMPQPMALVSLSEADRNALRAGASRDAIHADLLELYQKVNAKL
                     DPHEKIKTIVVTRGDWTIENGIMTPTLKIKRNVLESRYEDRIESWYESSEKVQWE"
     gene            complement(2140..2595)
                     /gene="sixA"
                     /locus_tag="VFX11_09830"
     CDS             complement(2140..2595)
                     /gene="sixA"
                     /locus_tag="VFX11_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX4938964.1"
                     /translation="MQLYIMRHGDAVQYARTDAERPLSDLGRQQVARMLDHIRLQPPV
                     RVIASPYLRAQQTARILCDGLGMVPVETVDGITPDNDPFEALKTIEQYRADPLLMVSH
                     QPLVGSLLGLLLEGSVSAGQMMGTGSVALLSCPYIGAGQAQLQWLQHPR"
     gene            complement(2610..3668)
                     /locus_tag="VFX11_09835"
     CDS             complement(2610..3668)
                     /locus_tag="VFX11_09835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX4938965.1"
                     /translation="MSIPSEKIAVLGGGSFGTAIANIVADNGHSVTLWMRDEDVVESI
                     RTQHENTRYLPGKKLNPSILPTTDLREAVQDTTIIFVAIPSKAFRAVLQQARPFLHPG
                     HILVSTTKGIEEDGFLMMSQILEQESPCGKIGVLSGPNLAKEVAQRVLTGTVIASEHE
                     DVRTRVQDVLSCNYFRVYANNDVFGVELAGALKNIYAIASGMASALGMGENTKAMLIT
                     RSLAEMSRFAARLGGNPMTFLGLAGVGDLFVTCTSPLSRNFQVGFGLGQGKPLDDILA
                     NLGGTAEGVNTIRLVKKQSVELEVYMPLVHGLHEIIFEGKQLKDVIKRLMLGEQNSDV
                     EFVLPGPDKLASFKKAKA"
     gene            complement(3719..4039)
                     /locus_tag="VFX11_09840"
     CDS             complement(3719..4039)
                     /locus_tag="VFX11_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938966.1"
                     /translation="MVEISIKYPLSRETALELREPILAVVLDSLKSVQVHDEGYTLNF
                     GRESQVIKPLAQMIEIERVVNSFLRLQLIVESNQGPIKLDLSGPAGTRDFLVTEFGLK
                     RWLK"
     gene            complement(4165..>4783)
                     /locus_tag="VFX11_09845"
     CDS             complement(4165..>4783)
                     /locus_tag="VFX11_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020304959.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX4938967.1"
                     /translation="KEGQDKIYYITAENYNAAANSPHLEIFRKKGIEVVLMYDRIDEW
                     MMGQLFDYKGKSFINIAKGDLDLGSIETEEDKKQQEEVEKSSEELVKRLKTALESRVS
                     DVKVSHRLTDSPACLVAGAYDMGIQMRQIMEAAGQKLPETKPTLEINPAHPLMERLNG
                     EVQDDRFGDLAMIVFDQAALSEGRALEDPSAYVKRINALLMETLH"
BASE COUNT          959 a         1480 c         1321 g         1023 t
ORIGIN      
        1 agtggattcc tgcagcgttg tacgatcagc aatgacctgc tgggattgcg cgccctgctt
       61 cacttccagt agcaacacat ccgcctggct gacaacccgg tccacatggg ccggcagttt
      121 cacgatatcc cggtccgcca catggatggt gccaaaaata tgattctgct tcccttcctt
      181 ttcgatctgc cacagcaggc tggcggatgc gagcggcgcc agcaacagcg catacaggca
      241 ggccaaccat atcagcgtcg atcgagtgaa gtgcacgatg ttcataagcc acaacactcc
      301 ggggaacaaa cagcctgttc attcccactg tactttttcc gatgattcgt accagctctc
      361 gatgcgatcc tcgtaacggg attccagtac attgcgtttg attttcagcg tgggggtcat
      421 gatgccattt tcaatcgtcc agtctccccg ggtcaccacg atggtcttga ttttctcatg
      481 cgggtccagc tttgcattca ctttctggta cagctccagc agatcggcgt gaatggcgtc
      541 gcggctggca ccggcacgca acgcattgcg atcagcttcc gacaacgaca ccagcgccat
      601 tggctggggc atggcgttgc ccaccacgca gcactgctca acgtgggaat ccgccagcag
      661 cttcgcctcg atgggaaacg gcgccacata cttgcccttg ctggtcttga agatttcctt
      721 gatacgcccg gtgatgcgca ggctgccgtc cgcctcgatt gcacccacat cccccgtgcg
      781 caggaaacca tcggcatcca gggcttccgc cgtcttttcc ggctccttgt aataacccac
      841 catggtggtg ggcgcgcgca ccagaacttc gccaccatcg cccagcttta catccaccga
      901 cggattgggt tgccccacat agccggggcg gccgcgcccc agacgcgtgg tatgggaata
      961 gccgaaattc tccgtcatgc cgtagacctc gatgatctcg atgcccagct ccttgtacca
     1021 ggccagcagg ctgttggaga tcggcgcagc gccgctcagg caaagccgcg cctgatccag
     1081 tccgaggccg tgccggattt tctttttgat gtacccggac aggcccggca cgcgaaacag
     1141 cagacgcagt tttttctcgg gaaattttgc aaacacgcct gcctggaact tcgaccagat
     1201 gcgcggcact gcgaaaaaca gcgtaggccg cgcccgttgc agatcctggg cgaaagtgtc
     1261 gagtgattcg gcgaagtgca catgggtacc ctggtaaatc tgcatcatct cgatgcaggc
     1321 ccgttccgcc acgtgcgaca gcggcagata ggacaacagg cggtcgcggc tggcgatttc
     1381 atagatatcc gatcccagcg aagcgccggc agcgatgttg ttgaacgtgt gcatcacgcc
     1441 cttgggcacg ccggtggtgc ccgaggtgta aatgatcgtg gccagctcac cgtggctgcg
     1501 cgacggattc tccgtcaacg gcgcatgctg ggccaccagg tcgtcccagg tgggaaaatc
     1561 cgtgggcggg ctgaggggat aactgacgca gcggatagcg ctgtcgacgc cggatttcat
     1621 gctgtcccag ccgtccagct tgcccacgaa caggatcttc gattcgctgt gctgcagaat
     1681 ctggcgcacc gaatctgcgg tcagggtggg gtacagtggc accgatacgc ctccggccat
     1741 ccagatcgcc agatccgaca gaatccacca ggcgcaattc ttggacagga tagcaatacg
     1801 gtcgcccggc ttcacgccaa tcgacttcag ataggccgcc atcgaacgca cctgacgacc
     1861 ggcctcggcc caggtcatgt ccttgatcac cccgttgccc atgggctggg tgaaacagat
     1921 cttggcgggc tgattttttt cccagaagta aaaccgctcc agcggcaggg gaatgccggt
     1981 gttggcagca ccgctcggac gtgatttcaa aggggcggtt accgtcatgt tcacctcgca
     2041 tcgtctcttg ttttagttgt tatcagcacg cgcattcaaa gcgaatatga cgaatgattt
     2101 caccaaacgc aagggaggta acctgcgccc gcgggacaat cagcgcgggt gctgcagcca
     2161 ctgcagttgt gcctgccccg ccccgatgta cgggcaggac agcagggcca ccgaacctgt
     2221 gcccatcatc tgtcccgccg aaacactgcc ttccagcagc aggcccagca gggagcccac
     2281 caggggctgg tggctgacca tcaggagcgg gtccgcccgg tactgctcga tggttttcaa
     2341 cgcttcgaag ggatcgttgt cgggcgtgat gccgtccacc gtttccaccg gcaccatgcc
     2401 caggccgtcg cacaggatgc gcgcagtctg ctgggcccgc aggtacgggc tggctatcac
     2461 gcgtaccggc ggctgcagcc ggatatggtc cagcatgcgc gccacctgct gccggcccag
     2521 atcactgaga ggacgctccg cgtcggttcg cgcatattgc acggcatcgc cgtgacgcat
     2581 gatgtaaagc tgcatgcagg cttgctccct tatgcctttg ccttcttgaa cgaggccagt
     2641 ttgtccgggc ccggcagcac aaattccaca tcggaattct gctcacccag catcagtcgc
     2701 ttgatcacgt ccttcagctg cttgccctcg aaaatgattt catgcaatcc gtgcaccagc
     2761 ggcatgtaga cttccagctc aactgactgc ttcttcacca gacgaatggt attgacgcct
     2821 tcggcagtgc cgcccagatt cgccaggata tcgtccagcg gtttgccctg gcccaggcca
     2881 aagcccacct ggaaattgcg gctcagcggc gaggtgcagg taacgaacag atcgcccacg
     2941 ccggccaggc cgagaaaggt catgggattg ccacccagtc tggcggcgaa gcgactcatt
     3001 tccgccagac tgcgggtgat cagcatggcc ttggtgttct cgcccatgcc cagcgccgac
     3061 gccatgccgg acgcaatcgc ataaatgttt ttcagcgcgc ccgccagctc cacgccgaac
     3121 acgtcgttat tggcatacac gcgaaaataa ttgcacgaca gcacgtcctg cacccgcgtg
     3181 cgcacatctt catgctcgct ggcgatgacg gtgccggtca gcacgcgctg cgccacttcc
     3241 ttcgccaggt tggggccact gagcacaccg atcttgccgc agggactttc ctgttccagg
     3301 atctggctca tcatcagaaa gccgtcttcc tcgatgccct tggtggtgct caccaggatg
     3361 tgaccggggt gcagaaacgg gcgggcctgc tgcaatacag cgcggaaagc cttgctggga
     3421 atcgccacga atatgatggt ggtgtcctgc acggcctcgc gcagatcggt ggtgggcaga
     3481 atggaaggat tgagtttttt acccggcagg tagcgggtat tttcatgctg ggtgcggatg
     3541 gattcgacga catcctcatc ccgcatccac aaggtgacac tgtgcccatt gtcggcgacg
     3601 atattggcga tagccgtgcc aaaacttccg ccccccagta ccgcaatttt ttcagacggt
     3661 atcgacatac aactccctgg cagtattttc ttgttgaatc gggggacccg ctgcgccgtt
     3721 atttcagcca gcgtttcagg ccgaactcgg tcaccagaaa atcacgcgtg ccagccggcc
     3781 cgctcagatc cagcttgatc ggcccctgat tggattccac gatcagttgc agacgcaaaa
     3841 aagaattcac cacccgttcg atttcgatca tttgcgccaa tggtttgatg acctggcttt
     3901 cgcgaccgaa attcagggtg taaccctcgt catgaacctg caccgatttc agtgaatcca
     3961 gcaccacggc cagaatcggt tcacgcagtt ccagtgcagt ttcccggctc aaaggatact
     4021 tgatcgatat ttcaaccaca cgcccctcct gagatgtccc cgctgaatga acacattcta
     4081 gcaggtaccc cgcctgaata aacgcattga aatgacataa aaaaggcgcc cttaaagcgg
     4141 cgcctttata cccatttcac cggatcagtg cagggtttcc atcaacagcg cgttgatgcg
     4201 cttgacgtaa gcggaagggt cttccagcgc acggccttcc gacagggccg cctgatcaaa
     4261 cacgatcatg gccaggtcgc cgaagcggtc atcctgcact tcgccattga ggcgctccat
     4321 cagcggatga gcgggattga tctccagagt gggcttggtt tccggcagtt tctggccggc
     4381 ggcctccata atctggcgca tctggatgcc catgtcgtaa gcacccgcca ccagacaggc
     4441 cggggaatcg gtcaaccggt gcgatacctt cacgtcggag acgcgggatt ccagtgccgt
     4501 tttcagacgc ttcaccagtt cttcggacga cttctccact tcttcctgct gcttcttgtc
     4561 ttcctcggtt tcgatagaac ccagatccag atcgcccttg gcgatgttga tgaaggactt
     4621 gcccttgtaa tcgaacagct gtcccatcat ccactcgtcg atgcgatcgt acatcagcac
     4681 aacttcaatg cccttcttgc ggaagatttc cagatgcggg ctgttggcgg cggcgttgta
     4741 gttttccgca gtgatgtagt agatcttgtc ctggccttcc ttc
//
LOCUS       DASUEG010000522         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_11236, whole genome shotgun sequence.
ACCESSION   DASUEG010000522 DASUEG010000000
VERSION     DASUEG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11236"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..711)
                     /locus_tag="VFX11_09850"
     CDS             complement(454..711)
                     /locus_tag="VFX11_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938968.1"
                     /translation="MGKPSKEELAMALAEAGRMREQGEDEHFVAKSLLNHNYRLKMYE
                     DLYHAIEHYIRSGQSDVEHGKLMRALDKIRKDEEHPGLERR"
     regulatory      824..1021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1138..>3054
                     /locus_tag="VFX11_09855"
     CDS             1138..>3054
                     /locus_tag="VFX11_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4938969.1"
                     /translation="MNPMKTVALFAVVACMPPAFAASQLPEVEVVSEAPVHADNIEQV
                     DLESFTGFGRQIDRDQFQQQMATVSDVLDQTSGVQIRQVGGHGGFATVSMRGTGGKQV
                     NLYLDGLLLNSAQTGGADLNLIPTAIIDRIEIYPDFTPVQLSNSNLGGAVNIVSVNLF
                     EPRREASVTQGSFGAEQYSLAVADALADWNFFLGLNSEASDNDYDIVNTRGTVNNPDD
                     DIEEAQNNAAYDQQSALLKTGTRWGQHQSVEALLNWSHADREIPAIDNNAVNDAALDS
                     DDWRGQLKYNDRYGPVAASWRVFTGRRLDLYDDRNGTAGIGNEYVETREEHWGAAGIF
                     AVDWWLNTTTLNLEYRDEDIAQVDRVTDLTALDNQRTQTLLGLQDDLFLLDDTLLFSV
                     AVRSQAYRDKVQLDPQQLQVFDGDAIAGGSDTETGASFGTQYQLTDALKLKANLATQS
                     RVPTLQERFGVLGVSVGNPELTPETSENRDIGAIWEKDGLMLRYVFYAKDLEDAIVVE
                     VNGQGIAKAKNVAEAEVSGHEMEASWQVHERAALNLGATQLDSENHSAIKADNGQQLP
                     GIYHSTFNAGADLNWRQWLLRMQYQYEDELYYDSANSVWPDAKETVNAQLRWSQERWS
                     VQLGIDNLLDESYQD"
BASE COUNT          642 a          860 c          901 g          651 t
ORIGIN      
        1 agcgattcaa tggcataggc tggaacctgc tccagctgtc atgtccaccg ggtggacagg
       61 cgctggatat cggcatcgct cacctgcaca ctgcctggag ctgcttcgtc tttgccacgc
      121 cagtgtgagc ccaggaacag aagtccgcct gccacgttga tatgcagttc actatattgc
      181 gattgcggat cgtggagggc atgcactgcg catcctgtcg cggattggga gtgggttttt
      241 gttgttggta tgccatcgcc atttgaatgt ccctgacaga cataccgcac agcggcagct
      301 tttggatcaa tggtaatgag gagggcgact ggagccttga ttgaaatcag gttttctgtc
      361 gtgcggagat ctttgaagca gggggcaaac gcaagtgagc cccatggtgt acaggctcac
      421 gtctgttgtc acgtcgcgcc agggcgcacc aagtcagcgg cgttccagtc cggggtgttc
      481 ttcgtccttg cggatcttgt ccagggcacg catcagcttg ccgtgctcca cgtcactttg
      541 gccggagcgg atatagtgct cgatggcgtg atagagatcc tcgtacattt tcaggcggta
      601 gttgtggttt aacaaagact tagccacgaa atgctcatct tcgccctgtt cccgcatgcg
      661 gccggcttcc gccagggcca tggctaattc ctctttgctg ggcttgccca tgatgaatcc
      721 tcgacatacc gaatctgttt catatcagtc tagccctccg caccggtttt gcccgcttgc
      781 acaatcgcaa cagtcccgcc cttgactaat cggacgcaga ccataaactg ggcgcctcga
      841 tggttccttc gcaggattaa aagggaacac ggtgacgcaa gcctcccctt gccgagtccg
      901 tggctgttcc cgcaactgta agcgaagagt gattttcaac gacggccact gggcaaccgg
      961 gaaggccgaa aatcgcgatg acgcgctagc caggagacct gccatcacac ggatgccccg
     1021 gcattcgtgc tcgtcaccta ccagtaacgg gtatttattg gtgcggctac cgtcatggtg
     1081 acgatattgg cgctgcctgt gggcggcttg atatcggttg tcgttgcaga cctgtctatg
     1141 aaccctatga aaacggtggc gttgttcgct gttgtcgcct gcatgccccc ggcatttgct
     1201 gccagtcagc tgccggaagt cgaagtcgtc agcgaagcac ccgtgcatgc ggacaatatc
     1261 gaacaggtcg atctggaatc ctttaccgga tttggccgcc agatcgaccg tgaccagttt
     1321 cagcagcaga tggccacggt cagcgatgtg ctggaccaga cctcgggcgt gcagattcgc
     1381 caggtgggtg gccatggtgg ttttgccacg gtttccatgc gcggcacggg cggcaagcag
     1441 gtcaatctct atctggatgg cctgttgctc aacagcgcgc aaaccggcgg cgccgatctc
     1501 aatctgattc ccactgcgat catcgaccgc atcgaaatct atccggattt cacgccggtg
     1561 cagttgagca attccaatct gggcggcgcc gtcaacatcg tatccgtcaa tctgtttgag
     1621 ccgcgccggg aagccagcgt gacacaaggt tcgtttggtg ccgaacagta cagcctggcg
     1681 gttgcagacg cgctggcgga ctggaatttt tttctgggcc tgaacagcga ggcctccgac
     1741 aacgattacg acatcgtcaa tactcgcggc actgtgaaca atccggatga cgatatcgaa
     1801 gaagcgcaga acaacgcggc ctacgaccag cagtcggcat tgctgaaaac cggtacgcgc
     1861 tggggacagc atcagtcggt ggaagcgctg ctcaactgga gccatgccga ccgggaaatt
     1921 cccgccatcg acaacaacgc cgtcaacgat gccgcgctgg attcggacga ctggcgcggc
     1981 cagctcaaat ataacgaccg ctatggacct gtcgctgcat cctggcgtgt cttcaccggg
     2041 cgccggctgg atctttatga tgatcgcaac ggcaccgccg gtattggcaa tgaatatgtc
     2101 gaaacgcgcg aggagcactg gggcgctgcc ggcattttcg ccgtggactg gtggctcaat
     2161 accaccacac tcaatcttga ataccgtgac gaggatatcg cccaggttga ccgggtgacg
     2221 gatctgaccg cgctcgataa ccagcgcacc cagaccttgc tcggtctgca ggacgatctg
     2281 tttctgctgg atgacacact gctgttcagt gtggcggtgc ggtcgcaagc gtatcgcgac
     2341 aaggtccagc ttgatccgca gcagctgcag gtgtttgacg gcgatgcgat cgcaggcggc
     2401 tccgatacgg aaaccggcgc ttccttcggt acgcagtacc agctgacaga tgcgctcaag
     2461 ctgaaagcca atcttgccac gcagtcgcgt gttcccaccc tgcaggagcg ctttggtgtg
     2521 ctgggcgtgt ccgtgggtaa tcccgagttg actccggaaa ccagcgaaaa ccgtgacatc
     2581 ggcgccatct gggaaaagga cggcctgatg ctgcgttatg tgttttatgc caaggatctg
     2641 gaagacgcca tcgtcgttga agtgaacgga cagggtattg ccaaggcgaa aaatgtggca
     2701 gaggccgaag tgagcggcca cgaaatggaa gcgtcctggc aagtgcatga aagggccgca
     2761 ctcaacctgg gtgccaccca gctggattcc gaaaatcatt ccgccatcaa ggccgacaac
     2821 ggccagcagt tgccgggcat ttatcacagc acctttaacg cgggcgccga tctgaactgg
     2881 cgccaatggc tgctgcggat gcagtaccaa tacgaagacg agctgtatta cgactcggcc
     2941 aacagtgtct ggcccgatgc caaggaaacg gtcaacgcac aattgcgctg gtcgcaggag
     3001 cgctggagtg tgcagctcgg catcgacaat ctactggatg agtcctatca ggat
//
LOCUS       DASUEG010000523         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_734130, whole genome shotgun sequence.
ACCESSION   DASUEG010000523 DASUEG010000000
VERSION     DASUEG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734130"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFX11_09860"
     CDS             <1..635
                     /locus_tag="VFX11_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX4938970.1"
                     /translation="GKPLTTLHHLRQQTAKANDQPNFSLADYVAPAGSKPDYIGGFVV
                     TAGIGLEKKIAEFEANHDDYSSILLKALADRLAEAFAERLHERVRKEFWGYVPTEALS
                     NEELIKEKYQGIRPAPGYPACPDHTEKGTLFRLLDAENKTGVTLTEHFAMYPTAAVSG
                     WYFSHPESRYFAVGKIGKDQVENYASRKGMTLEQAERWLSPNLAYDPDAT"
     gene            653..1990
                     /locus_tag="VFX11_09865"
     CDS             653..1990
                     /locus_tag="VFX11_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4938971.1"
                     /translation="MTQYWPSGDRLRQILTLALPIIAGMVSQSLLNLADAWIVGQLGS
                     NALAAVGMGGNTNYLASAAVIGIGAGVQAMVARRKGEDNAGAMATPLNAGLLVGILAS
                     IPLFVLFYSGSGWLMSVLIDDPAVTPLADEYFSVRVIGMFALAMNFSFRGYWNGINRS
                     MVYMRTLVIMHVADLAISYALVFGLWGLPKMGVYGAGLGTTLALYLASALYLLQCWQH
                     SRPHGFLRKMPSRALLVNTLKLSLPNSIQQVFFAGGLTVLFWILAQIGTPELAVGHVL
                     ITLILFLILPAMGLGLSATSLVSQALGRKDAEDAVRWGVNITLLGMAMLWSIALPLLL
                     FPDFILSQFLTEPALIELGRMSLRITALFIGIDAAGIILTQCLLGAGASGLVMRTSIL
                     FQWGFFLPVAWLIGPVLGFGLTAVWLLQMVQRLLLALTMAYHWRGRRWAAIRI"
     gene            2025..2255
                     /locus_tag="VFX11_09870"
     CDS             2025..2255
                     /locus_tag="VFX11_09870"
                     /inference="COORDINATES: protein motif:HMM:NF022622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX4938972.1"
                     /translation="MTQDSDKHDSSNKAGILGVLQTVLGAVFGIQSEKKRQEDFAKAD
                     PGKLIAIGIVTVIIIMVTMALIVRSALESAGH"
     gene            2439..>4007
                     /locus_tag="VFX11_09875"
     CDS             2439..>4007
                     /locus_tag="VFX11_09875"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX4938973.1"
                     /translation="MYIYTEYDQKLLTERVAQFRSQTERFLKGDLTNEEFLPLRLQNG
                     LYVQRHAPMLRIAVPYGMLDSAKLRVLAEIARDFDKGYAHFSTRQNVQFNWPELSQVP
                     AILAKLATVQMHGIQTSGNCIRNTTTDQFAGVARDEIEDPRPWCEIIRQWSTFHPEFA
                     FLPRKFKIAVNAAADDRAAIKFHDIGVQIVKNAKGETGFSIWVGGGLGRTPIVAEEIT
                     SFLAPRYLLSYLEAILRVYNRYGRRDNKYKARIKILVKALGIDGFREKVEAEWQHMKD
                     TEMDLPESEISRMKGFFREPAYAQLQDNPPVFADARKSSPGFDNWVRRNCTAHKKPGY
                     MAVTLSLKATAVAPGDVSTDDLFAIADLADRFSFGEVRSTHNQNVVLADVRQDQLFDL
                     WQQLKALGFATANIGYLTDITCCPGGDFCSLANAKSIPIAEQIQRRFDDLDYLYDLGE
                     IELNISGCMNACGHHHIGNIGILGVDKKGEEFYQISLGGNSGTNAKIGQIMGPSFGEA
                     DVADVIAKIIDVYVE"
BASE COUNT          822 a         1188 c         1168 g          829 t
ORIGIN      
        1 acggcaagcc gcttaccacc ctgcatcatc tgcggcagca aaccgccaag gccaacgacc
       61 agcccaattt ttcattggcg gattatgtag caccggccgg cagcaagccc gattacattg
      121 gcggctttgt ggtgacggcg ggcatcggtc tggaaaagaa aatcgccgag ttcgaagcga
      181 atcacgacga ttacagttcc atcctgctga aagcgctggc ggaccgtctg gcggaagcct
      241 ttgccgaacg tctgcacgaa cgcgtgcgca aggaattctg gggctatgtg ccgaccgaag
      301 ccctcagcaa cgaagagctg atcaaggaaa agtaccaggg tatccgtccg gcgcccggct
      361 atcccgcctg cccggatcac accgaaaaag gcaccctgtt ccggttgctg gatgcagaaa
      421 acaaaaccgg tgtgacgctc accgagcatt tcgccatgta tcccactgca gcggtcagcg
      481 gctggtattt ctcgcatccg gaatcacgtt atttcgctgt cggcaaaatc ggcaaggatc
      541 aggtggaaaa ctacgcttcc cgcaagggca tgacgctgga acaggcggaa cgctggctga
      601 gcccgaacct ggcctatgac ccggatgcaa cataaatgat acgacacaac gcatgaccca
      661 gtattggccc tcgggagatc gcctgcgcca gattcttacc ctggcgctgc cgatcatcgc
      721 cggcatggtg tcgcagagcc tgctcaacct ggctgatgcc tggatcgtgg gccaacttgg
      781 cagcaatgcg ctggccgccg tgggcatggg cggcaacacc aactacctgg catcagctgc
      841 ggtgattgga atcggcgcgg gtgtgcaggc gatggtggcg cgacgcaagg gcgaagacaa
      901 cgccggcgcc atggcaacgc cgctgaatgc cggtctgctg gtgggcatcc ttgcttcgat
      961 tcccctgttt gtcctgttct attccggttc cggctggctc atgtccgtgc tgatcgatga
     1021 tcctgcggtg acgccgctgg cggatgagta tttctcggtg cgggtgatcg gcatgttcgc
     1081 cctggcgatg aatttcagtt ttcgcggcta ctggaacggt atcaaccgct cgatggtgta
     1141 catgcgcacg ctggtgatca tgcatgtggc ggatctggcg atcagctatg cattggtgtt
     1201 cggcctgtgg gggctgccaa aaatgggtgt gtatggcgcc ggactcggca ccacccttgc
     1261 gctgtacctg gccagtgcgc tgtacctgct gcaatgctgg cagcattccc ggccccacgg
     1321 ctttctgcgc aaaatgccca gccgtgcatt gctggtgaat accctcaaat tgtcgctgcc
     1381 caattccatc cagcaggtgt tctttgccgg cggcctcacc gtgctgttct ggattctggc
     1441 gcagatcggc acgccggaac tggcggtggg ccatgtgctg atcacgctga tcctgttcct
     1501 gatcctgccg gcgatggggt tggggctatc ggccacctct ctggtgagcc aggcgctggg
     1561 acgcaaggat gccgaagacg ctgtgcgctg gggcgtgaac atcactctgc tgggtatggc
     1621 catgctctgg agcatcgccc tgcccctgct gctgttcccg gatttcatcc tcagccagtt
     1681 tttgactgaa ccggccctca tcgaactggg ccgcatgtcg ttgcgcatca ccgccctttt
     1741 catcggcatc gacgccgccg gtatcatcct cacccagtgc ctgctgggag ccggtgccag
     1801 cggcctggtg atgcgcacca gcattctgtt ccagtggggc ttctttctgc cggtggcctg
     1861 gctgatcggg ccggtactgg ggttcggact taccgccgtc tggttgctgc agatggtgca
     1921 acgcctgttg ctggccttga ccatggccta tcattggcgg ggccggcgct gggcagccat
     1981 ccggatatga cacccctttc cttcccctat ttaggacgtc actcatgaca caggattccg
     2041 acaagcacga ttccagcaac aaggccggca ttctgggggt attgcagacc gttctggggg
     2101 ccgtcttcgg cattcagagc gagaagaagc gccaggagga ctttgccaag gccgatcccg
     2161 gcaagctgat cgccattgga atcgtcactg tcatcatcat catggtgacc atggcgctga
     2221 tcgtgcgctc ggcgctggaa tccgcagggc attgagcgga aacggaaact tatcaacagg
     2281 ttccgttatt catagaaccg catattcata tgtcaaaata ttacttttct gtataaagac
     2341 gtgctggtaa cctgcacgcc tcgaaaatcc atccaccccg tgtttccgcg acaaaaacca
     2401 tatgtttcat atggttacaa gttgtaacga gtgaaggcat gtacatctat accgagtacg
     2461 accaaaagct gctgaccgag cgcgtagcgc aattccgcag ccagaccgaa cgcttcctca
     2521 agggcgacct caccaacgaa gaattcctgc ccctgcgtct gcagaacggc ctgtacgtgc
     2581 aacgccatgc gcccatgctg cgcatcgccg tgccctacgg catgctggac agtgcaaagc
     2641 tgcgggtgct ggcggaaatc gcgcgggact tcgacaaggg ctatgcccat ttcagtaccc
     2701 gtcagaacgt gcagttcaac tggcccgaac tgtcccaggt accggctatc ctggccaagc
     2761 tggcgaccgt gcagatgcat ggcatccaga ccagcggcaa ctgtatccgc aacaccacca
     2821 ccgaccagtt tgctggcgtg gcgcgggatg aaatagaaga tccccgcccc tggtgcgaaa
     2881 taatccggca gtggagcacc ttccacccgg aattcgcctt cctgccgcgc aagttcaaga
     2941 ttgctgtcaa cgcagccgca gatgaccgcg ccgccatcaa attccatgac atcggcgtgc
     3001 agatcgtgaa aaacgccaag ggcgaaaccg gcttttccat ctgggtgggc ggtggcctgg
     3061 gacgtactcc gatcgtggct gaggaaatca ccagcttcct tgcgccccgc tacctgcttt
     3121 cctatctgga agccattttg cgtgtctaca accgctatgg ccgtcgcgac aacaagtaca
     3181 aggcccgcat caagatactg gtgaaggcgc tgggaatcga tggtttccgc gagaaagtgg
     3241 aagcggaatg gcagcacatg aaagacacgg aaatggacct gccggaatcg gaaatcagcc
     3301 gcatgaaagg ctttttccgc gagccggcct atgcgcagct gcaggacaat ccgccggttt
     3361 tcgccgacgc acgcaagtcc agccccggtt ttgacaactg ggtgcggcgc aattgcactg
     3421 ctcacaagaa gccgggctac atggcggtga ccctgtcact gaaagcgact gccgtggcgc
     3481 cgggcgacgt cagcacagac gatctgtttg ccatcgccga cctggccgac cgcttcagct
     3541 tcggcgaggt gcgcagcacc cacaaccaga atgtcgtgct ggcggacgta cgtcaggatc
     3601 agctgttcga tctgtggcag caactgaaag cgctggggtt tgccaccgcc aacattggat
     3661 acctgaccga tattacctgc tgcccgggcg gcgacttctg ttcgctggcc aacgccaagt
     3721 ccattcccat cgcggagcag atccagcgcc gattcgacga cctggattac ctgtacgacc
     3781 tgggtgaaat cgagctgaac atttccggct gcatgaacgc gtgcggtcac caccacatcg
     3841 gcaacatcgg cattctgggt gtggataaaa agggcgaaga gttctaccag atttccctgg
     3901 gcggcaattc gggaaccaat gccaagattg gtcagatcat ggggccctcg ttcggagaag
     3961 ccgacgtggc ggatgtgatt gccaagatca tcgatgtcta tgttgaa
//
LOCUS       DASUEG010000524         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_48086, whole genome shotgun sequence.
ACCESSION   DASUEG010000524 DASUEG010000000
VERSION     DASUEG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48086"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFX11_09880"
     CDS             <1..500
                     /locus_tag="VFX11_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938974.1"
                     /translation="FGPGDVAPTPTGNIIVTMINGGTRFKFPGGFNVVDVRDVAAGHI
                     LAAEKGRSGQLYILGNENIAGEEFCRQCARILGRSERQIELPKLLVSGGALLGEWVAN
                     NVTHKKPYIVPGELKYSSQFIYMDCSKARRELGYNPRDIRISLEDSIHWFQQHHAERR
                     RQSAA"
     gene            620..1399
                     /locus_tag="VFX11_09885"
     CDS             620..1399
                     /locus_tag="VFX11_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX4938975.1"
                     /translation="MQWIISITTALLVFIVGRFLVKVITRLVSHAMRVGKMDELLVNF
                     LSSIIQWALMLFVIIAALEQLGINTTSLVALLGAAGIAIGLALKDSLQNFSSGVMLII
                     FRPFKNGDLIEAAGVTGVVEHIGIFTTNLKTGDNKEVIVPNGQVYGSTITNYSAKPTR
                     RIDLVFAISYDSDFRKAKDILSGILAKDTRVLRDPAPTVAVGELGEKSVNLLVRPWVK
                     SGDYWDVCWNINEQVRAEFEAAGIKSPAPQMNFHLHPAEQQ"
     gene            complement(1446..1655)
                     /locus_tag="VFX11_09890"
     CDS             complement(1446..1655)
                     /locus_tag="VFX11_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4938976.1"
                     /translation="MANASTGKVKWFNESKGFGFIEQDNGPDVFVHFSAITGSGFRTL
                     TEGQRVQFNVTQGQKGPQAENVQVI"
     gene            complement(1860..2150)
                     /locus_tag="VFX11_09895"
     CDS             complement(1860..2150)
                     /locus_tag="VFX11_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4938977.1"
                     /translation="MQKGLGHYKNAMTHFTVLEENDTSSLLTIQIETGRKHQIRRHLA
                     EMGHPVLGDARYGDAAPGGLHLAAVHLCYQCPVQGRRLDLDVSPQKIGRFWQ"
BASE COUNT          526 a          616 c          596 g          514 t
ORIGIN      
        1 cgttcggccc cggcgacgtg gcacccaccc ccaccggcaa catcatcgtc accatgatca
       61 acggcggcac ccgcttcaag tttcccggcg gcttcaatgt ggtggatgtg cgcgatgtgg
      121 cggccggcca tatcctggca gcagaaaaag gccgcagcgg acaactctac atcctgggca
      181 atgaaaacat tgctggcgag gaattctgtc gccagtgcgc ccgcattctt ggccgcagcg
      241 agcgccagat cgaattgccc aaactgctgg tgtccggcgg cgcactactg ggggaatggg
      301 ttgccaacaa tgtcacccac aaaaagccct atattgtgcc gggcgagctg aaatacagct
      361 cccagttcat ctacatggac tgcagcaagg ccagacgcga actgggctac aacccgcgcg
      421 acatacgcat atccctggaa gattccatcc actggttcca gcagcatcac gccgagcggc
      481 ggcggcagtc cgctgcctga acccgcttac gcaggtctgg acttgcagga aagcaggcgt
      541 agcattgcca gaattaccaa caggagggca ctacatgatc gagcaataca taccaaaaga
      601 gttttcacag gaaatactga tgcaatggat catcagtatc acaaccgcat tactggtgtt
      661 catcgtcgga cgcttcctgg tgaaagtcat taccaggctg gtgtctcacg ccatgcgcgt
      721 gggcaagatg gatgaactgc tggtgaactt cctgtcttca atcatccagt gggcgctgat
      781 gctgtttgtc atcatcgccg cgctggaaca gctgggcatc aacaccacct cgctggtggc
      841 cctgctgggt gctgccggaa ttgccatcgg tctggcgctg aaggattcgc tgcagaattt
      901 ttcctcaggc gtcatgctta tcatctttcg cccgttcaag aacggagatc tgatcgaggc
      961 tgccggcgtc accggcgtgg tggaacacat cggcattttc accaccaatc tgaaaaccgg
     1021 tgacaacaag gaagtcatcg tgcctaacgg gcaggtgtat ggcagcacta ttacgaacta
     1081 ttccgccaaa cccacccgtc gcatcgatct ggtttttgcg atttcctacg acagcgattt
     1141 tcgcaaggcc aaggacattc tgtccggaat tctggcaaaa gatacgcgcg tgctgcggga
     1201 tcccgcaccg actgtggcgg tgggtgaact gggagagaaa tcggtcaacc tgctggtgcg
     1261 gccgtgggta aaatccggcg actactggga cgtctgctgg aacatcaatg agcaggtcag
     1321 ggcagaattt gaagccgcag gaataaaatc gcccgcgccg cagatgaatt ttcacctgca
     1381 ccctgccgaa cagcagtaaa aacgcagaag ggtgaagttt ttggcttcac ccttctttgc
     1441 tttacttaaa taacctgtac gttttcagcc tgcgggcctt tctggccttg tgtcacgttg
     1501 aactgaactc gttgaccttc ggtcagtgtg cggaaacctg aaccagtgat tgcactgaaa
     1561 tgaacgaata catcggggcc gttatcctgt tcaataaaac caaaaccttt ggattcgttg
     1621 aaccatttaa ctttgcctgt tgaagcattt gccataaaat tttcctgact tttaccaaaa
     1681 gaatttacga agtacccgaa ggtgttgaga cacgacgcaa ggctggcggc aggagttact
     1741 gatgatgatc acgacgagaa aatctaaggg gcttttcgtt ttgaacaatc actttggcac
     1801 actgctttgg gcaatgcttt accgattata gccttaacca cttgcccgtc aacggggatt
     1861 tactgccaga aacggccgat tttctgtggc gaaacatcca gatccaaccg gcgcccctga
     1921 acagggcatt gatagcaaag gtgaaccgca gccaggtgca atccgcctgg tgccgcgtct
     1981 ccataacggg catcgcccag gaccggatgc cccatttcag ccagatgccg ccggatttga
     2041 tgcttgcgcc cggtttcgat ctgaatagtc agcagactgc ttgtgtcatt ttcttctaac
     2101 acggtgaaat gcgtcatggc gtttttgtaa tgacccagcc ctttctgcac aggtcaggcc
     2161 gctaatgcat acgcctcagc gggtgttttc attcccagcg cctggtgtgg ccgttgggtg
     2221 ttgtagaact gaatccagtc gctgattaca cg
//
LOCUS       DASUEG010000525         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_642909, whole genome shotgun sequence.
ACCESSION   DASUEG010000525 DASUEG010000000
VERSION     DASUEG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642909"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFX11_09900"
     CDS             <1..988
                     /locus_tag="VFX11_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938978.1"
                     /translation="VMLQLPAGQPHPVATFHVPDRFPADLARILIQAWGVLTERAFTR
                     IDEHGQLTLCLGLSATHYFVSGRADFNKMIQGGTSVLDVEASNRFIQRGPTNRRDSYE
                     TNDVWGPQINRTVQVPDGGAIQYKIEQHLSDKQTEEKDKYPQYQCEVVNTSPGGFCLT
                     WKQDVPPQVKTGEIVGIMESDKNHWSIGVIRWVKQFKNEGARMGVELIAPKADACGTQ
                     VVHKKGGVTEYMRTLMLPELRAIGQPATLITPNIAFHVGYKVNISENGAVSKAQLIKQ
                     VTSTASFSQFQFKMLVPAAQPAESATAIPPPPAPAPARSEQDDDFDSIWSSL"
     gene            1071..>2417
                     /locus_tag="VFX11_09905"
     CDS             1071..>2417
                     /locus_tag="VFX11_09905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX4938979.1"
                     /translation="MSKKAETIKLLILHESRDDAEQLMNLIRNSGRATRGQLIDSADT
                     LLSALTGSQWDLMLLRPEAEGMSALECFNQAKKLDKDIPAILLIDENDSDEITDALLN
                     GFEDAVPHDQDQRLMLVIQRELRNLGERRQRRVADFNLKEAEKRCRVLLDSSRDAITY
                     VTDGMHTYANQAYLELFGYDDAEELEGMPIMDMVAPKDHDNFKNFLRAYSAGESDSNE
                     FVCHGICTDGTEVQARMIFSNATYDGEPCTQILIRTNQADAQLAERLKEISSQDLLTG
                     LFNRQHFNQELDRTLQWAATSSKTAVIFYIQLDNFTAIQSDVGMAAADMVISDVASLL
                     KELLPENSLLARFGDEVFTAIIKEQSLEEGAALAEKIRATLEDHMSEVQERTIQVTAS
                     IGVTLISENSDNGQEVITRVHQASEQVREGKPLGNGIKVFDPASIKKIDANDTGAIV"
BASE COUNT          579 a          766 c          671 g          401 t
ORIGIN      
        1 ggtgatgctg caattgcctg ccggccagcc ccacccggtc gccaccttcc atgtgccaga
       61 ccggtttccc gcagatctgg cccgcatcct gatccaggcc tggggcgtac tcaccgagcg
      121 cgccttcacc cgcatcgacg aacacggcca gctgaccctg tgcctgggcc tgagtgccac
      181 tcattatttc gtgtccggcc gtgccgattt caacaagatg atccagggcg gcaccagcgt
      241 gctcgatgtg gaagccagca accgcttcat ccagcgcggc cccaccaacc gccgcgacag
      301 ttacgaaacc aacgatgtct ggggccccca gatcaaccgt accgtccagg ttcccgacgg
      361 cggcgccatc cagtacaaga tcgagcagca cctcagcgac aaacagaccg aggaaaagga
      421 caagtacccg cagtaccagt gcgaggtggt caacaccagc cccggcggat tctgtctgac
      481 ctggaagcag gatgtgcctc cgcaggtgaa aaccggggaa atcgtcggca tcatggaaag
      541 cgacaagaat cactggagca tcggcgtcat ccgctgggtg aaacagttca agaacgaagg
      601 tgcgcgcatg ggggttgaac tgattgcgcc caaagcagat gcctgcggaa cccaggtggt
      661 gcacaagaaa ggcggcgtca ccgaatacat gcgcacgctg atgctgccgg agctgcgcgc
      721 catcggccag cccgccacgc tgatcacgcc caacatcgcc tttcacgtgg gttataaggt
      781 caatatttcc gaaaatggcg ccgtgtccaa ggcccagctg ataaaacagg tgacatcgac
      841 tgccagtttc agccagtttc agtttaaaat gctggtgcct gccgcacagc cggcggaatc
      901 tgcaaccgcg atcccgcccc cgccggcacc cgcacctgcc cggtcagaac aggacgacga
      961 tttcgattcc atctggtcca gcctgtagaa aaggaagtgc aggatgctga ataatgaaaa
     1021 cgcgtgccca cttttcccgg ttgccattgg cctgttgaat tcctgatccc atgtcgaaaa
     1081 aagccgagac catcaagcta ctgatcctgc atgaatcgcg cgacgacgcg gaacagctca
     1141 tgaatctgat ccgtaattcc ggccgtgcca cccggggtca gttgattgac agcgccgaca
     1201 ccctgctgag tgccctcacc ggcagccagt gggacctgat gctgctgcgc cctgaagccg
     1261 agggcatgag cgcgctggag tgtttcaacc aagccaaaaa actcgacaag gatattccgg
     1321 caatcctgct gatcgacgaa aacgattccg acgaaatcac cgatgcgctg ctcaacggat
     1381 ttgaagatgc tgtgccccac gaccaggacc agcgcctgat gctggtgatc cagcgcgaac
     1441 tgcgcaacct gggtgaacgg cgccagcgcc gggtggcgga cttcaacctg aaggaagcgg
     1501 aaaagcgctg ccgcgtcctg ctggacagct cacgcgatgc gatcacctac gtgaccgacg
     1561 gcatgcacac ctacgccaac caggcctatc tggaactgtt cggctacgac gacgccgaag
     1621 aactggaagg catgccgatc atggacatgg tggcgcccaa ggatcatgac aatttcaaga
     1681 atttcctgcg cgcgtacagt gccggtgaaa gcgattccaa tgaattcgtc tgccacggca
     1741 tctgcaccga tggcaccgaa gtgcaggcgc ggatgatttt ctccaacgcc acctacgacg
     1801 gcgaaccctg cacccagatc ctgatccgca ccaaccaggc cgatgcccag ctggcggaac
     1861 gcctgaagga aatcagcagc caggatctgc tcaccggcct gttcaaccgc cagcatttca
     1921 accaggagct ggatcgcacc ctgcagtggg ctgccaccag cagcaaaacc gccgtcatct
     1981 tttacatcca gctcgacaat ttcacggcca tccagtctga cgtaggcatg gcggcagcag
     2041 acatggtgat cagcgacgtg gcgtccctgc tgaaggaact cctgccggaa aacagtctgc
     2101 tggcccgttt cggtgacgag gttttcaccg cgatcatcaa ggagcagagc ctcgaggaag
     2161 gcgccgcact ggcagaaaaa atccgcgcca cactcgaaga ccacatgtca gaagtacagg
     2221 agcgcacgat ccaggtcacc gccagcattg gcgtcaccct gatcagcgag aacagcgaca
     2281 acgggcagga agtcatcacc cgcgtgcacc aggccagcga gcaggtacgc gaaggcaaac
     2341 ccctgggcaa cggcatcaag gtattcgatc ctgccagcat caagaaaatc gatgccaacg
     2401 acaccggtgc catcgtg
//
LOCUS       DASUEG010000526         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1950266, whole genome shotgun sequence.
ACCESSION   DASUEG010000526 DASUEG010000000
VERSION     DASUEG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950266"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2109
                     /locus_tag="VFX11_09910"
     CDS             <1..>2109
                     /locus_tag="VFX11_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4938980.1"
                     /translation="SLSMLTLVVALFFQSNNWERQRLESVFHSQARTVAENLKHDIDR
                     YQDAVAEVRRLYESSTQVTLREFQHFTAELLERSPGIRALEFVPLVHDADRLSFERRA
                     QAEGLSGFSIREFHKDGTVGPAHQRPEYYPVYQLAPLEGNEMALGLDMGSDPSRRTAV
                     EQARTTRRSTATPGIVIVNTRQLGVLLFSPVFVTPGSNNDVFRGVAMAALDLKNVVEL
                     GVPDAVANDVLINLIDVTTPDSPQPLYTDSREPPRETLMEWRIPLRFANRDWQLHLQA
                     STAFLNEQRSLISWLVLAGGLLLTGILQILLLMMASHTRSVEARVRERTRALVHSEER
                     FRAVFNDNPDAILVVDSRGHIVGSNARAVSLFGYDRATLEQASIELLMPERWRQIHEQ
                     HRKGFFTAPQVRAMGAGLALFGRRSDGKEFPVDVMISPMHLGDETLAIAIVRDITERK
                     RLEQEREESEARFRAVAESASDALISSDAQGYIVFANPAAEKMFGYPQDQLLGLPLSQ
                     LMPVHQELENADGPHEAHALAAARTGKRHDGGEFPVEVSEATWSFGGARYSTTIIRDI
                     TQRRAQEERLNSTLREKDMLLKEVYHRVKNNLQVIQSLLNLESRSLSDEPARQALADM
                     GTRIRAMALVHEKLYQSRNLASISLSDYIHDLLHQLQNSGAIDSSRIRVQVNVSNIDV
                     GLESAIPLGLLLNELVSNAF"
BASE COUNT          437 a          646 c          622 g          404 t
ORIGIN      
        1 cagcctgagc atgttgaccc tggtggtggc gctgttcttc cagtcgaaca actgggaacg
       61 gcaacgcctg gagtcggtct ttcacagcca ggcgcgcacg gtagccgaga acctcaagca
      121 tgacatcgac cgttaccagg atgccgtagc ggaagtgcgc cggctctacg aaagttcaac
      181 ccaggtcact ctgcgggaat tccagcactt caccgccgag ttgctggagc ggtcacctgg
      241 catccgtgca ctggaattcg tcccgctggt ccacgatgcc gatcgcctgt cctttgaaag
      301 gcgggcacaa gcggaaggtc tgagcggctt ttccatacgc gaatttcata aggatggcac
      361 cgtcggcccg gcccaccagc gccccgaata ctaccccgtc taccagttgg ctcctctgga
      421 gggcaacgag atggcgctgg gcctggacat gggctccgac ccatcaagac gcacggcggt
      481 ggaacaggca cgcaccaccc ggcggtccac tgccacgccc ggcatcgtga tcgtgaatac
      541 ccgacagttg ggtgtactgc tgttcagtcc cgtcttcgtt acgcccggga gtaacaacga
      601 tgtcttcagg ggggtggcca tggcggcgct ggacctgaag aacgtggtgg aacttggtgt
      661 gcctgacgcc gttgcaaacg acgtgttgat caacctgatc gatgtcacca cgccggattc
      721 acctcaaccg ctatataccg attcacgcga acctcctcgc gaaacactga tggagtggcg
      781 aattcccctt cggtttgcca atcgtgactg gcagcttcac ctccaggcca gcacggcgtt
      841 cctgaacgag cagcgctcac tgatatcgtg gctggtgctg gccggcggcc tgctgctgac
      901 gggcatcctg caaatcctgt tgctgatgat ggccagtcac acgcgcagcg tggaagcgcg
      961 cgttcgcgaa cggacgcgcg ccctggtaca cagcgaggaa cgcttccgtg ccgtcttcaa
     1021 cgacaatccc gatgcaattc tggtggtgga cagccgcggc cacatcgtag gatcgaatgc
     1081 gcgcgccgtc agcctgttcg gctatgaccg cgccacactg gagcaagcct cgatcgaatt
     1141 gctcatgccc gagcgctggc gccagattca tgagcagcac cgcaagggat ttttcaccgc
     1201 gccacaggtg cgggccatgg gtgccgggct ggcgctgttc ggtcggcgtt cggatggcaa
     1261 ggagtttccg gtcgacgtga tgatcagccc catgcatctg ggcgacgaga cgctggccat
     1321 tgccatcgta cgcgacatca cggagcgaaa acggctggaa caggagcgcg aggaaagtga
     1381 agcgcgcttt cgcgcagtgg ccgaatctgc cagtgatgcc ctgatctcca gcgatgccca
     1441 gggttacatt gtgtttgcca acccggccgc cgagaaaatg ttcggctatc cgcaggacca
     1501 gttactggga ctgccattgt cacagctgat gcctgtccac caggagctgg aaaatgcaga
     1561 cggcccacat gaggcacatg cattggccgc tgccagaacc gggaaacgcc atgatggcgg
     1621 cgagtttcct gtcgaggtta gcgaggcaac ctggtcgttt ggaggcgcgc gctactccac
     1681 caccatcatc cgcgacatta cgcagcgccg tgcacaggaa gagcgcctca acagcacgtt
     1741 gcgtgaaaag gacatgctac tgaaagaagt ctatcatcga gtcaagaaca acctgcaggt
     1801 catccagagc ctgctcaatc tggagtcgcg cagcctttcc gacgaaccgg cccgccaggc
     1861 gctggccgac atgggaactc gcatccgtgc catggcactg gtccatgaga aactttacca
     1921 gtcgcgcaat ctcgccagca tttcactctc cgactacatt catgacctgc tgcaccaact
     1981 gcagaattcc ggtgcgatcg acagcagccg cattcgtgtg caggtgaatg tatcgaatat
     2041 tgatgtcggt ctggaatcgg ccatcccgct gggactgctg ctcaacgaac tggtctcgaa
     2101 tgccttcaa
//
LOCUS       DASUEG010000527         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1306641, whole genome shotgun sequence.
ACCESSION   DASUEG010000527 DASUEG010000000
VERSION     DASUEG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306641"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFX11_09915"
     CDS             <1..315
                     /locus_tag="VFX11_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX4938981.1"
                     /translation="YLITSGWLDIHRGEVVGLVVETKCTYHASVAFPDELDVGLRVER
                     LGNSSVRYGIGIFRKGDVEAAAEGYFVHVYVNRDTNAPVKVPDALRAVLEPLLVSEGE
                     QA"
     gene            318..1772
                     /locus_tag="VFX11_09920"
     CDS             318..1772
                     /locus_tag="VFX11_09920"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4938982.1"
                     /translation="MVLTRPELLKSRALVDGVWCNADDGSSFAVTNPADGSVITTVPR
                     LQAAETGRAIAAADKALSGWRNKTAKERAQILRRWYELMVANQNDLAAILTHEQGKPL
                     AESRGEVLYGAAFLEWFGEEAKRIYGDVIPGFTADRRLLVFKQGIGVVAAITPWNFPI
                     AMITRKVGPALAAGCTVVVKPASETPLSALALAALAEEAGVPPGVINVVTGPAGVIGP
                     VLTQSPTVRKLSFTGSTDIGRRLMAESADTLKKLSLELGGNAPFIVFDDADLDAAVRG
                     AMLSKFRNSGQTCVCANRFLVQDSVYDAFVEKFAAAVAALKVGAGWEPDVSQGPLINE
                     AALNRVHGLLDDMQGDGASIVCGGSPHERGGLFFEPTIVRDVRNDSELAQSELFGPIA
                     PVIRFKDEAEAIRLANDTEFGLASYFYSQNINRIFRVSEALEYGMVGVNEGIISTEVA
                     PFGGVKASGLGREGSKYGIDEYLEIKYVCIGGVS"
     gene            complement(1835..2539)
                     /locus_tag="VFX11_09925"
     CDS             complement(1835..2539)
                     /locus_tag="VFX11_09925"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX4938983.1"
                     /translation="MTAPGIKFFSTTAHACSYLPGKQAVTLFADPKSNMDRRLYTELS
                     DLGFRRSGNYVYRPHCGACQACVAIRIPVDRFSPNRQQLRVWKKNQDLDVIIRDATFC
                     EEHYELYERYICARHRDGDMFPPSVEQYASFLTSDWSETCFIEFRLEGKLLAVAVCDE
                     LENGLSAVYTFYDADEEKRSLGTYAILWQIEESQRRGLPSLYLGYWIRECQKMSYKIA
                     FRPIELLINGEWLVAR"
     gene            complement(2607..3266)
                     /gene="aat"
                     /locus_tag="VFX11_09930"
     CDS             complement(2607..3266)
                     /gene="aat"
                     /locus_tag="VFX11_09930"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX4938984.1"
                     /translation="MDEALDDPNGLLAIGGDLEPDRLLLAYRHGIFPWFSPGEPICWW
                     SPDPRCVFLTEGWRPSRSLAKTLRKDYFRFTVDQCFTQVMESCAAPRHYADGTWIQPE
                     FIRSYTKLHRRGIAHSIEVWNPDGAMVGGLYGLNLGRLFFGESMFSTETDASKAAFAY
                     LMQLCQSWSIPLIDGQVENPHLLRLGAQLIPRREFVAWLEASAGLPAPDWHSAPPFRQ
                     P"
     gene            complement(3350..4300)
                     /gene="trxB"
                     /locus_tag="VFX11_09935"
     CDS             complement(3350..4300)
                     /gene="trxB"
                     /locus_tag="VFX11_09935"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX4938985.1"
                     /translation="MSAQHHRLIILGSGPAGYTAAVYAARANLSPVVITGMQAGGQLT
                     TTTEVDNWPGDAHGLTGPALMERMQAHAERFGTKVVFDHINEVDFATRPYRLKGDSGE
                     YTCDALIIATGASAQYLGLPSEQEYMGRGISACATCDGFFYKGQRVMVVGGGNTAVEE
                     ALYLSNIADHVTLVHRRDKLKSEKILQDHLFAKEKDGKISIIWNHQLDEVLGGDAGVS
                     GARLKSTADGSTQDVDVAGIFIAIGHKPNTDIFAGKLDMANGYLKVKGGSEGNATATN
                     VPGVFAAGDVADHVYRQAITSAGAGCMAALDAEKYLDNLQ"
     gene            4621..>5491
                     /locus_tag="VFX11_09940"
     CDS             4621..>5491
                     /locus_tag="VFX11_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX4938986.1"
                     /translation="MRETIAIFWGLIGLYLLVALMTWSPNDSGWSRIGETTTSNMGGD
                     FGAWLSDILISAVGHVAILIPVFIFIQVANLWRKEPPLMGLPWRLLSLVLSLLALAAL
                     AGLHFSTPTPELRNAAGGVFGFEVAHSLAAAFGVFGGTLLLLAVALVGITVATGLSWF
                     WLMDIVGHAVWDLGAIIASVLNGQFTAQRDAWRERRAMKKALEEAAPSFELSLSNAKV
                     KAQISREETVRAEPVLAPMPEPVRKARPKAIEERVAPVITPMEAPVKKSERAQKEKQR
                     SLFDAMPVSGDLPS"
BASE COUNT         1115 a         1552 c         1726 g         1098 t
ORIGIN      
        1 tacctgatca cgtccggctg gctggacatt catcgcggtg aggtcgtggg tctggtagtg
       61 gaaacgaagt gtacctatca cgcctccgtg gcattcccgg acgagctgga tgtcggtctg
      121 cgcgtggagc gccttggcaa ctccagcgtt cgttatggca tcggcatttt ccgcaaaggg
      181 gatgtcgaag cggctgccga aggctacttt gtgcatgtct acgtcaaccg ggatacaaat
      241 gcgccagtga aagtgccgga tgcgcttcgt gccgtgctgg agccgctatt ggtcagtgaa
      301 ggagaacagg catgacactg gtactgactc gccccgagct gttgaaatca cgcgcactgg
      361 tcgatggcgt gtggtgcaat gcggatgatg gcagcagctt tgccgtcacc aaccccgcag
      421 atggctcggt catcacgacc gtgccacgac tgcaggctgc tgaaaccggg cgcgccattg
      481 cagctgccga caaggcgttg tcaggctggc gcaacaagac tgcaaaggaa cgtgcccaga
      541 tcctgcggcg ctggtacgag ctgatggtgg ccaatcaaaa cgatctggcc gccatcctga
      601 cgcatgagca gggcaagcca ctggccgaat ccaggggcga agtattgtat ggcgcggcgt
      661 ttctggagtg gtttggtgaa gaggcaaagc gcatctatgg tgatgtgatt cccggattta
      721 ccgcagaccg tcggctcctg gttttcaagc agggaattgg ggtggtcgcc gccatcacgc
      781 catggaattt tccaattgcc atgattaccc gcaaggtagg gccggcactg gcggcgggat
      841 gtacggttgt ggtcaagcct gcttcggaaa cacctctgtc ggcactggca ctggcagcgc
      901 tggcggagga ggcgggtgtt ccccctgggg tcatcaatgt tgtgactggc ccggccggcg
      961 tgattggtcc tgtactgacc caaagcccga ccgtgcgcaa gctgtcattc acagggtcga
     1021 cggacatcgg caggcgcctg atggcagagt ccgccgacac gctgaaaaag ctgtctctgg
     1081 aactgggtgg caatgcgcct ttcattgtgt tcgatgatgc cgatctggac gctgcggtac
     1141 gcggtgccat gctgtcgaaa ttccgcaact cggggcagac ctgtgtgtgc gccaaccgtt
     1201 tcctggttca ggacagtgtt tacgatgcct ttgtagagaa atttgccgct gccgtggcag
     1261 cactgaaggt gggtgctggc tgggaaccgg atgtcagtca gggaccactc atcaacgaag
     1321 cggcattgaa tcgtgtgcac ggcctgctgg atgacatgca aggcgacggg gcttcaatcg
     1381 tgtgtggcgg gagcccgcat gagcgtggcg gactcttttt cgaacccacc atcgtgcgtg
     1441 atgtgcgcaa tgacagtgag ttggcacaaa gcgaactttt cggccccatc gctcctgtta
     1501 tccgcttcaa ggatgaggcc gaggccatcc gccttgccaa tgacaccgag tttgggctgg
     1561 cctcttattt ctacagccag aacatcaacc gcatttttcg cgtatcagag gcgctggaat
     1621 acggcatggt gggcgtcaat gaaggcatca tcagtaccga agtggcgcca ttcggcggag
     1681 taaaggcctc gggcctgggc agagagggct cgaaatacgg tatcgatgag tacctggaga
     1741 tcaagtacgt ttgcattggc ggggttagct gagcccacct ctgatgaaat gaaacgggcg
     1801 ccatctggcg cccgtttcgt tcagcatgac gtcattagcg tgcaactagc cactcaccat
     1861 tgatcagtag ttcaatgggt cggaaagcaa tcttgtagct cattttctgg cactcgcgga
     1921 tccagtaacc gagatacagg ctgggtaggc cgcgacgctg ggactcttcg atctgccaca
     1981 gaatggcgta ggtgcccaga ctgcgctttt cctcgtcggc atcatagaac gtatacacag
     2041 ctgacaatcc gttctccagc tcgtcacaca cggcaacggc cagcagcttg ccttccagcc
     2101 ggaactcgat aaagcaggtt tcggaccagt ccgatgtcag aaaggacgcg tattgctcaa
     2161 cgctgggagg aaacatgtcg ccatcgcgat ggcgcgcgca gatatagcgc tcatacagct
     2221 catagtgttc ttcgcagaag gtcgcatcac gaatgatgac gtccagatcc tggttcttct
     2281 tccagaccct gagctgttga cgattgggtg aaaaacggtc gaccggaata cggatggcca
     2341 cacaggcctg gcaggcaccg cagtgcggcc gatagacata attcccgctg cgacggaagc
     2401 cgagatcgga aagttcggta taaagccggc ggtccatatt ggatttggga tcggcaaaca
     2461 gcgtgacggc ctgcttgccc ggcaggtagc tgcaggcgtg tgccgtggtg ctgaagaact
     2521 tgatccccgg ggccgtcatc tcatgcctct cgcttttctt gtttttatag cacagcgaca
     2581 gcgtctgtcg gcaatacaac gccaacttaa ggctgacgaa acgggggcgc agagtgccag
     2641 tcgggggcag ggagaccagc cgaggcctcc agccaggcca caaactcgcg tcgggggatc
     2701 aactgcgccc ccaggcgcag caggtggggg ttttccacct gcccatcgat caggggaatc
     2761 gaccagctct ggcagagctg catgaggtac gcaaaggcag ccttggatgc atcggtttcc
     2821 gtgctgaaca tggactctcc gaagaagagg cgaccgaggt tgaggccgta aagcccgcca
     2881 accattgctc cgtcagggtt ccagacctca atggagtggg caatgccacg cctgtgcagt
     2941 ttggtgtagg agcggatgaa ctccggctgg atccaggtgc cgtcggcata gtggcgggga
     3001 gctgcgcagg actccatgac ctgggtaaag cactggtcta cggtaaacct gaaatagtcc
     3061 tttcgcaggg tcttggccag actccgggag gggcgccagc cttcggtcag gaacacgcag
     3121 cgaggatcag gcgaccacca gcagatgggt tcgcccggac tgaaccaggg gaatatgcca
     3181 tggcgatacg ccagcaacag ccgatccggc tccaggtctc cgccgatggc cagcaggcca
     3241 ttggggtcgt ccagcgcctc gtccacaggc gggaacccgt actcgatggg gcctaagcgg
     3301 tagagcggca cagagggagt aggcaggggc tggagcccct gccgggacct tactggaggt
     3361 tgtcgaggta cttctcggca tccagggccg ccatgcagcc ggcacctgcc gaggtaatgg
     3421 cctgacggta gacatggtcg gccacgtcac cggcagcaaa cacgccaggc acgttggtcg
     3481 cagtggcgtt accctcgctg ccgcccttca ccttgaggta gccgttggcc atgtccagct
     3541 tgccggcaaa gatgtcggta ttgggtttgt ggccgatggc aatgaagatg cccgcgacat
     3601 caacatcctg agtgctgccg tccgccgtgc tcttgaggcg ggcgccgctc acgccagcat
     3661 cgccgcccag cacttcgtcc agctggtggt tccagatgat ggatatcttg ccgtccttct
     3721 ctttggcaaa gaggtgatcc tgcaggatct tctctgactt cagcttgtcg cggcgatgca
     3781 ccagggtgac atggtcagcg atattggaca ggtacagggc ctcttcaacc gcggtattgc
     3841 caccacccac caccatgacc cgctgcccct tgtagaagaa gccgtcacag gtggcgcaag
     3901 cgctgatgcc gcgccccatg tactcctgct cggagggcag gcccagatac tgggcagaag
     3961 cgccggtggc gataatcagg gcgtcacagg tgtattcgcc actatcgccc ttgaggcggt
     4021 aggggcgggt agcaaagtcg acctcattga tgtggtcgaa caccaccttg gtgccaaagc
     4081 gctcggcatg ggcttgcatg cgttccatca gtgcagggcc ggtaaggcca tgggcatcac
     4141 ccggccaatt gtccacctcg gtcgtcgtcg tcaactggcc tccggcctgc atgccggtga
     4201 tgacaaccgg gctgaggttg gcacgggccg cgtagaccgc agcggtgtaa ccggccggac
     4261 cggagccaag gataatcaga cggtggtgct gggcggacat gtcatctcca tgatcgggaa
     4321 agccagccat gacgagcggg ctgtggataa acgggggcac caggcagttg ccccggggaa
     4381 aggcgccaat cttagcctca attgatgggg acgtgctgcc tgtatatcaa cattgcgctt
     4441 aagacagcct gaattgccgg atacccgtca caaaccaccg acagatcagt ggcttgaacc
     4501 agaaagaagc catggttcgc atggcataat ccggtccagc cgttacaata gcgccttgtt
     4561 ttcgcagaga ccgcccagtt gagcaccacg gacgcccgcc aacccgcgcc ccgcaagggc
     4621 atgcgcgaaa ccattgccat tttctggggc ctgatcggcc tctacctgtt ggtcgcgctg
     4681 atgacctggt caccaaatga ctcgggctgg agccgcattg gcgaaaccac gaccagcaac
     4741 atgggagggg acttcggcgc ctggctgtcc gatatcctga tcagcgctgt cgggcatgtc
     4801 gccatcctca ttccagtttt catcttcatc caggtggcca atttgtggcg caaagagccg
     4861 ccgctaatgg ggttgccctg gcgcctgctt agcctcgtgc tgtcgctgct ggcgctggct
     4921 gccctggcag gtctgcactt cagcacaccg acgccggaac tgcgcaacgc ggcaggcgga
     4981 gtgtttggct ttgaggtggc acattcgcta gccgcagcgt tcggtgtctt cggcggcaca
     5041 ctgctattac tggcggttgc tctggtcggg attacggtag cgaccggtct ttcatggttc
     5101 tggttgatgg acatcgtggg acatgccgtc tgggacctgg gcgccatcat tgccagtgtt
     5161 ctcaacggac agttcactgc ccagcgtgat gcctggcggg aacgacgtgc catgaagaag
     5221 gcgctggaag aagccgctcc gtcctttgag ctgtcgttga gtaacgccaa ggtcaaagcc
     5281 cagatttcga gggaagagac cgtgcgtgcc gaacctgtgc tggcacccat gccagagccc
     5341 gtccggaaag ccagaccgaa ggcgatcgaa gagcgtgtag cccctgtcat tacaccgatg
     5401 gaagcccccg tcaaaaagag tgagcgggcc cagaaggaaa aacagcggag cctgtttgat
     5461 gccatgccgg tgagcggtga cctgccgtca c
//
LOCUS       DASUEG010000528         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1268038, whole genome shotgun sequence.
ACCESSION   DASUEG010000528 DASUEG010000000
VERSION     DASUEG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268038"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFX11_09945"
     CDS             <1..706
                     /locus_tag="VFX11_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913596.1"
                     /note="catalyzes amidations at positions B, D, E, and G on
                     adenosylcobyrinic A,C-diamide. NH(2) groups are provided
                     by glutamine, and one molecule of ATP is hydrogenolyzed
                     for each amidation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HEX4938987.1"
                     /translation="PRISNHTDFDPLRLHPQVEFHYVRAGEKLPPADLIILPGSKNTL
                     ADLHWLREQGWAAQIQRHVRFGGKLLGICGGFQMLGQQLLDPHGIEGTQRSAEGLGWL
                     EMTTELQPHKQLHRRSGRLLLPQENGGAQSAVQCEGYEIHAGISIGPALQSSLVRFDN
                     GDTDGALSGCGQIAGTYLHGVLDHPEALQAVLAWSGLAQAEKLDYRALREQQIDRLAD
                     ATAQHLDLAAIKKILA"
     gene            complement(748..1491)
                     /locus_tag="VFX11_09950"
     CDS             complement(748..1491)
                     /locus_tag="VFX11_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEX4938988.1"
                     /translation="MSDILSPPLVWPALGTLTVTPHHSLFRANAVMQATGSCVWNGGS
                     ALVHGWLNQKVAKTAHAKLEVPELSLQRYAQELGIDTPLAGMMTAASMKSLRFQLIQD
                     QEMTLACVVTAGIENARRSGDPCDGDFSHRKLTQTGTINIVLVSNTAFAPAARIEALM
                     VATEAKTAACYDLRMKSPVSGAPATGTGTDSICLLSGVSEQDALEYCGKHTRMGEWIG
                     RATYAAVRESVTACLDLLDSRCAQPDSVA"
     gene            complement(1499..2242)
                     /locus_tag="VFX11_09955"
     CDS             complement(1499..2242)
                     /locus_tag="VFX11_09955"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX4938989.1"
                     /translation="MKSLLIALQFLTRIPVPVKDCTPEQVRASIYWYGIAGLAIGAIL
                     LAAQTLLLWLLPWDADAAVAALLLTLWVLLTGALHLDGLADSADAWLGGHGDRERSLA
                     IMKDPQSGPAGVIAIVLVLLLKFSLVLYLQQHQQMFYLLLVPMLARAMMPFLFLFTHY
                     VREEGLGSPFAERLAVEKMLPGLVIAVVAGVLLSGLFWLVAVGLALAVFIAARALMVK
                     RIGGTTGDTAGALIEILEVTLLLGCIFIE"
     gene            complement(2248..>2485)
                     /locus_tag="VFX11_09960"
     CDS             complement(2248..>2485)
                     /locus_tag="VFX11_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4938990.1"
                     /translation="RVEQALRTVIAAHGDQHVLVVAHGGVVRASLSACFGFTSRATFC
                     VEVPYAAMTRIRAFVHEDGHTEYSLAFINGFTDH"
BASE COUNT          505 a          760 c          779 g          441 t
ORIGIN      
        1 gccgcgcatc agcaatcaca cggatttcga tccgctgcgg ctgcatccgc aagtggagtt
       61 ccactatgtg cgtgccggtg aaaaactgcc accggcggat ctgatcattc tgccgggcag
      121 caaaaatacg ctggcggatc tgcactggtt gcgggagcag ggttgggcag cgcagattca
      181 acgccatgtg cggtttggcg gcaagttgct gggcatttgc ggcgggtttc agatgctggg
      241 gcagcaattg ctggacccgc acggcatcga gggaacgcag cgcagtgcgg aaggactggg
      301 ctggctggaa atgacgacgg aactgcagcc gcacaaacaa ttgcaccggc gcagtggccg
      361 tttgctgttg ccgcaggaaa atggtggcgc acagagcgcg gtgcaatgtg aaggctatga
      421 aattcacgcc ggtatcagca ttggccctgc gctgcaatcg tcgctggtcc ggtttgacaa
      481 tggcgacacg gatggcgcgc tgtcaggctg cgggcagatt gccggcacct acctgcacgg
      541 tgtgctggat catccggagg cgttgcaggc agtgctggcg tggagcggtc tggcgcaggc
      601 ggaaaaactg gattaccgcg cgctgcggga acagcagatc gaccggctgg cggatgcgac
      661 ggcgcagcat ctggatctgg cagcgatcaa aaaaattctg gcgtagtcgt aacgattttt
      721 tgcagaggat tgttctgcaa ctgaaggcta cgcaaccgaa tccggttgtg cgcaacggct
      781 gtccagcaga tccagacagg ccgtcaccga ttcccgcacc gccgcatagg tggcgcggcc
      841 gatccattcc cccatccgtg tgtgcttgcc gcagtattcc agcgcatcct gttccgaaac
      901 gccgctgagc agacaaatgg aatctgtccc ggtgcccgtg gcgggtgcgc cggaaaccgg
      961 acttttcatg cgcagatcat agcaggcggc ggtcttcgcc tcggtggcca ccatcagtgc
     1021 ttcgatgcgg gccgccggtg cgaaggcagt attgctgacc agcacgatat tgatggtgcc
     1081 cgtctgtgtg agtttgcggt gggaaaaatc cccgtcgcag ggatcgccgc tgcggcgcgc
     1141 gttctcgatg ccggcggtga cgacgcaggc cagcgtcatc tcttgatcct ggatcagctg
     1201 aaaacgcagt gatttcatcg acgctgccgt catcatgccg gccagcggcg tgtcgattcc
     1261 cagttcttgc gcataccgtt gcagactgag ctccggcact tccagtttcg cgtgtgctgt
     1321 cttcgccact ttctggttca gccagccgtg caccaaggca ctgccaccgt tccatacgca
     1381 ggagccggtt gcctgcatga cagcgttcgc gcgaaacagg ctgtggtgtg gcgtgacggt
     1441 caatgtgccc aatgcgggcc aaaccagagg gggcgataga atgtcggaca tgccgttgtc
     1501 actcgataaa aatgcagccc agcagcaggg tgacttccag tatttcgatc agcgcgccgg
     1561 ccgtgtcgcc ggtggtgccg ccaatgcgct tcaccatcag cgcccgtgca gcaatgaaca
     1621 ccgccagcgc cagtcccacg gccaccagcc agaacagccc gctgagcagt acgcccgcca
     1681 ccactgcaat caccaatccc ggcagcatct tttccaccgc cagccgctcg gcaaacgggc
     1741 tgcccagacc ttcctcgcgc acgtaatggg tgaataagaa caggaacggc atcatcgccc
     1801 gcgccagcat cggcaccagc agcagataga acatctgctg gtgctgctgc agatacagca
     1861 ccagggaaaa tttcagcagc agcaccagca cgatggcaat cacacccgcg ggaccggact
     1921 gcggatcttt catgatggcg agagatcttt cgcgatcgcc gtggccgccc agccaggcat
     1981 cggcgctgtc cgccaggcca tccagatgca gcgcgccggt cagcagcacc cacagggtca
     2041 gcagcagggc cgccaccgcc gcgtctgcat cccacggcag caaccacagc agcagcgttt
     2101 gcgccgccag caaaatcgca ccgatcgcca gtcccgcgat gccataccag taaatcgacg
     2161 cccgcacctg ctccggcgtg cagtccttca cgggcaccgg aatgcgggtg agaaattgca
     2221 gggcgataag cagtgatttc atggtgatca gtgatccgta aaaccgttga tgaaggcgag
     2281 ggagtattcc gtgtggccgt cctcatggac gaaggcgcgg atgcgggtca tggctgcgta
     2341 gggcacctcc acgcagaatg tagcgcgcga cgtgaagccg aagcaagccg acagggatgc
     2401 gcggacgaca ccgccgtgcg ccaccaccag cacatgctgg tcgccatgcg cagcgatgac
     2461 ggtgcgcagt gcctgctcga cccgc
//
LOCUS       DASUEG010000529         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_276755, whole genome shotgun sequence.
ACCESSION   DASUEG010000529 DASUEG010000000
VERSION     DASUEG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276755"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..863
                     /locus_tag="VFX11_09965"
     CDS             153..863
                     /locus_tag="VFX11_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938991.1"
                     /translation="MTDERFKVWMTFWQFFLGTVVLGIVSTVISWQIQTREIEIKEQE
                     ASAKFLEQALQEDVGVRRRLAQYFSTVTRSEALRERWQEYARVIDDEFRETLAQKQEL
                     ELAASKAEDSQAREWLMGRVAQLDQALSPQSSMAPVSPRVYVHIVREDQRSKANAVAD
                     ALRTSAVIVPGVDLVKSGPSSSELRYFRRVEQAEAEGIASTISALWPGVTARYVAGYE
                     NSTGIRPRHFELWLSASP"
     gene            889..1215
                     /locus_tag="VFX11_09970"
     CDS             889..1215
                     /locus_tag="VFX11_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEX4938992.1"
                     /translation="MNRTLFHFLLALFGGGFALAFALICVPPFLENPDLLAAAAAGFV
                     NPYSTGYSLDAVFCWLVLTAWILHEAREKGIRHGWIAILLGMVPGVATAFAFYLWLRQ
                     RQYSGR"
     gene            1265..>2214
                     /locus_tag="VFX11_09975"
     CDS             1265..>2214
                     /locus_tag="VFX11_09975"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4938993.1"
                     /translation="MRERLLRVTLIIRLTAGAVLMAQVSLLQASDADSDTLVSEKGRI
                     TATLVVSGLKHPWGMAFLPDGRMLVTERTGTIRYVTSDGRKSGPLSGMPDIEVGGQGG
                     LFDVILDPDFGRNGWIYFSYSEQGDGGNSTAVARATLQENAIADLTVIFRQQPKFNSA
                     QHFGGRLVFTPDGHLFITLGERGRRSLDAQTLNTHHGKVVRIHPDGRIPVDNPFVQDP
                     AALGAIWSYGHRNVQGAALHPQTGQLWTHEHGPQGGDEINIIRPGKNYGWPVITYGEQ
                     YGGGRIGDTAKSGLEQPLHYWVPSIAPSGMAFYTADLFPA"
BASE COUNT          423 a          633 c          689 g          469 t
ORIGIN      
        1 acccccgtga attgagctga accaccgttt gaccctccct ctgtgccggc ctgctgtgta
       61 ataacggcag cgccggcact gtctttgcgt ttgcaaatag agctagaatc cggcgtagtt
      121 tccttctccc tttcctgacc gccggggttg cgatgacaga cgaacgcttc aaggtatgga
      181 tgacattctg gcaatttttc ctgggcactg tggtgctggg aattgtgtcc accgttatca
      241 gctggcagat ccagacgcgg gaaatcgaaa tcaaggagca ggaggccagc gccaaattcc
      301 tggagcaggc gctgcaggaa gatgtcggcg tgcgacggcg gctggcgcaa tatttttcca
      361 ccgtgacccg ttcggaggcg ttgcgcgaac gctggcagga atatgcccgg gtgatcgacg
      421 atgaattcag ggaaaccctg gcgcagaagc aggaactgga gctggccgcc agcaaggcgg
      481 aagattccca ggcccgcgaa tggttgatgg ggcgggtggc ccagctggat caggcattga
      541 gcccgcagtc gtccatggcg ccggtgtcgc cccgcgtgta cgtgcacatc gtgcgcgagg
      601 accagcgcag caaggccaac gcagtggccg atgcattgcg cacgtcagct gtgattgtgc
      661 ccggcgtgga tctggtgaaa agcggcccct ccagcagtga gctgcgttat ttccggcggg
      721 tggagcaggc cgaggcggaa ggcatcgcat ccaccatcag tgcgctctgg cccggcgtca
      781 cggcccgcta tgtggcgggc tatgaaaatt ccaccggcat ccggccccgg catttcgagc
      841 tgtggctgtc tgccagccca taatcagtcc ccataaaagg aagcgcgcat gaatcgtaca
      901 ctgttccact ttttgctggc gctgttcggt ggggggttcg cgctggcgtt cgccctgatc
      961 tgcgtgccgc catttcttga aaatcctgat ttgctggcgg ctgcggcggc gggttttgtg
     1021 aacccttatt cgacgggcta ttccctggac gcggttttct gctggctggt gctcaccgcc
     1081 tggattctgc atgaagcgcg cgagaaaggt attcgccacg gctggatcgc gattctgctg
     1141 ggcatggtgc ccggcgtggc caccgcgttt gcgttttatc tgtggttgcg gcagcggcaa
     1201 tattcgggcc gataatctct cgcaggttat caactcacag ttttccacga ttaaggaacc
     1261 caccatgcgc gagagacttt tgcgggtcac tttaatcatc cggttgactg ctggcgctgt
     1321 tttgatggct caagtcagcc tgttgcaggc cagcgacgct gactccgata cgctcgtcag
     1381 cgaaaaaggc cgcatcactg ctacccttgt cgtgagcgga ctaaagcatc cctgggggat
     1441 ggccttcctg cccgatggcc gcatgctggt aacggaacgc accggcacca ttcggtatgt
     1501 cacatccgat ggcaggaaaa gcgggccgtt gtccgggatg ccagacattg aggtcggcgg
     1561 gcagggcggt ctgttcgatg tgattcttga tcccgatttt ggcaggaatg gatggattta
     1621 cttcagttac agcgaacagg gagatggagg caacagcacc gctgtggcgc gggccactct
     1681 gcaggaaaat gccatcgcgg atctgacagt gatcttccgg caacaaccca aattcaatag
     1741 cgcgcaacat tttggcggcc gtctggtgtt cacgcccgat ggccatctgt tcatcaccct
     1801 gggtgagcgt ggccggcgct ccctggatgc gcagacactc aatacccatc acggcaaagt
     1861 ggtacgcatt catccggacg gccgcatccc ggtggacaat cccttcgtcc aagatccggc
     1921 ggcgttgggc gccatctggt cttacggtca ccgcaacgtg cagggtgccg cactgcatcc
     1981 acaaaccgga cagctgtgga cccatgaaca cggcccgcag ggcggcgatg agatcaacat
     2041 tatccggccg gggaaaaatt atggctggcc tgtcatcacc tacggtgaac aatacggtgg
     2101 cggcagaatt ggcgataccg ccaagagcgg cctggagcag ccgttgcact attgggtgcc
     2161 gtccatcgcg ccgagtggca tggccttcta cacggcggat ctgtttcccg cctg
//
LOCUS       DASUEG010000530         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1397850, whole genome shotgun sequence.
ACCESSION   DASUEG010000530 DASUEG010000000
VERSION     DASUEG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397850"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFX11_09980"
     CDS             <1..810
                     /locus_tag="VFX11_09980"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX4938994.1"
                     /translation="GALHGIMRVRAFTQDDGHIFCTEDQVQAEVTAFHQQAMKVYSDF
                     GFENIAVKLALRPDKRIGSEEAWDKAENGLRAALSSAGVQWEELPGEGAFYGPKIEYH
                     LKDSIGREWQVGTVQFDPMMPERLNAEYVDEHSQRQRPIMLHRAIVGSMERFIGILIE
                     HFAGHFPLWLTPVPVVVATITDDARDWAQSVVAQLKAAGIQAEADLRNEKIGYKVREH
                     SNAKVPQIWVVGKQEAAEQKVAIRQLGSQQNEVVTIVEALERIKAATAMPA"
     gene            1033..2424
                     /gene="cysS"
                     /locus_tag="VFX11_09985"
     CDS             1033..2424
                     /gene="cysS"
                     /locus_tag="VFX11_09985"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX4938995.1"
                     /translation="MTLHLHNTLTQQKEIFTPLNPNQVTMYVCGPTVYNFVHIGNARP
                     VVVFDTLFRLLQRHFPKVIYARNVTDVDDKINAAAKANNESIRDFAERYAIAYREDMG
                     NLNNLPPTLEPYATDNIDAMIEQIQALIKNGHAYEAEGHVLFQVSTDPQYGKLSKRNM
                     DDMIAGARVEVAPYKKEPADFVLWKPSSDDLPGWDSPWGRGRPGWHIECSAMISKHLG
                     KTIDIHGGGMDLIFPHHENEIAQGTCCHEHGQEAFVRYWMHNGYLNIDGEKMSKSLGN
                     FRTVRDLLQEWPGEVIRYALLTAHYRKPLDFSGDLLANAKSSLDRLYNTLRESPLAVD
                     TNVRHPQLDELEKHLQDDLATPQALAVLHALAGEINKSQGEEKIRLQKVLRKAGDCLG
                     LLNQDSESWFKWQPAGKTGGLSDAEIDALIVERNDAKKAKNFGRADEIRKQLKEQGIV
                     LEDSKDGTRWTRE"
     gene            complement(2439..3098)
                     /locus_tag="VFX11_09990"
     CDS             complement(2439..3098)
                     /locus_tag="VFX11_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4938996.1"
                     /translation="MKSDPTSQSAVADAKPALKTTYHHGNLREALVAAGLELLESTPA
                     DSLSLREIARQAGVSANAVYRHFADKDALLMALAAEGFRRLGVTQVAAREGAKDAVES
                     MLIAGRAYIHFARDNPALFRLMFGRFAKSHRTDELEQAEKRSIEPLLASVAAASKRDA
                     ADPRVVNGMVHAWALVHGLSHLLLEEQFTTLADDPEKLIEDVLLQASQRGGKRDGGNP
                     A"
     gene            3177..3608
                     /locus_tag="VFX11_09995"
     CDS             3177..3608
                     /locus_tag="VFX11_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4938997.1"
                     /translation="MQSTIAPVNESLAIWHEMIRSGDLSRLPDLLHPDAQFRSPMAYK
                     PYRSAQAVALILTTVARVFEDFHYHREFISSNGLDVVLEFSARVGDKGLKGIDMIQFD
                     ADGRITDFEVMIRPFNALQALGGAMAERLGAILPAFKGPGG"
     gene            3659..>3925
                     /locus_tag="VFX11_10000"
     CDS             3659..>3925
                     /locus_tag="VFX11_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005040406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX4938998.1"
                     /translation="MSLPRLLDDLAVNTVEWTARQIRKQVPYSPSNPFLEGPYAPVRE
                     ERTEAALRVTGHIPPELNGLYARIGPNPMQVENPATYHWFTGDGM"
BASE COUNT          893 a         1211 c         1151 g          670 t
ORIGIN      
        1 ggtgcgctgc acggcatcat gcgcgtgcgc gcgttcaccc aggacgacgg ccacattttc
       61 tgtaccgagg atcaggtgca ggcggaagta acggcattcc accagcaggc gatgaaggtg
      121 tactcagatt tcggctttga aaatattgcg gtgaaactgg cactgcgccc tgacaaacgc
      181 atcggcagcg aggaagcctg ggacaaggcg gagaatggcc tgcgggcggc cttgagcagc
      241 gcgggcgtgc agtgggaaga gttgccgggt gaaggcgcgt tttatggccc gaaaatcgaa
      301 taccacctga aggattccat cggccgcgag tggcaggtgg gcaccgtgca attcgatccg
      361 atgatgccgg agcgcttgaa cgcagaatat gtggacgagc attcccagcg ccagcgcccg
      421 atcatgctgc accgcgccat tgtcggctcc atggagcgct ttatcggcat tctgatcgag
      481 cactttgccg gacattttcc gctgtggctg acaccggtgc cggtggtggt tgcgactatt
      541 accgacgatg cgcgcgactg ggcacaatcg gtggtggcgc agttgaaagc ggcgggcatt
      601 caggctgaag cggatctgcg caacgaaaag atcggctaca aggtgcgcga gcacagcaac
      661 gcgaaagtgc cgcagatctg ggtggtgggc aaacaggaag ccgccgaaca gaaagtggca
      721 atccggcagc tgggcagtca gcagaatgaa gtggtgacga ttgtggaagc gctggaacga
      781 atcaaggcgg cgacggcgat gccggcgtaa agaaagaaga ggctggcaca cgcttgccag
      841 cggattgagg agcggtctgg gcggtttgca tttcgggacc gtttgcggcc agggatggcc
      901 gcggacgagc gcacatggat gtgcttgtag cgtgtcccga tatgcaaacc gcccagatcg
      961 ctccttgatc cgccagctcc gcatccaaaa ccgaaacgcg cacattacag aacccgaagt
     1021 tgaaccgaga ccatgaccct acacctgcac aacaccctga cccagcaaaa agaaatcttc
     1081 acgccgctga acccgaatca ggtcaccatg tacgtgtgcg ggcctacggt ctacaacttc
     1141 gtccacatcg gcaacgcgcg cccggtggtg gtgttcgaca cgttattccg cttgctgcag
     1201 cgtcacttcc ccaaggtgat ctacgcccgc aacgtcaccg atgtggacga caagatcaac
     1261 gccgccgcga aagccaacaa cgaatcgatc cgcgacttcg ccgaacgtta tgccatcgcc
     1321 tatcgcgagg acatgggcaa tctcaacaat ctgccgccca cccttgagcc ctacgccacc
     1381 gacaacatcg acgccatgat cgaacagatc caggcgctga tcaaaaacgg ccacgcatac
     1441 gaagcggaag gccacgtgct gttccaggtg agcaccgatc cccagtacgg caagctgtcc
     1501 aagcgcaaca tggacgacat gatcgccggc gcgcgggtgg aagtggcgcc ctacaaaaag
     1561 gaaccggcag acttcgtatt gtggaagcca tccagtgacg accttccggg ctgggacagc
     1621 ccctggggcc gcggccgtcc gggctggcac atcgaatgct ccgccatgat cagcaaacac
     1681 ctgggcaaga ccatcgacat tcacggcggc ggcatggacc tgatcttccc gcatcatgaa
     1741 aacgaaatcg cccagggcac ctgctgccac gaacatggcc aggaagcatt cgtgcgttac
     1801 tggatgcaca acggttatct gaatatcgac ggcgagaaaa tgtcgaagtc gctcggcaat
     1861 ttccgcaccg tgcgcgatct gctgcaggaa tggcccggcg aagtgatccg ttatgcgctg
     1921 ctcaccgcgc attatcgcaa gccgctggat ttcagtggcg atctgctggc gaacgcgaaa
     1981 tccagtctgg accgcctgta caacacgctg cgggaatcac cgctggccgt tgataccaat
     2041 gtgcggcatc cgcaactgga cgaactggaa aaacatctgc aggacgatct ggccacgcca
     2101 caggcgctgg cggtgttgca cgcgctggcg ggtgaaatca acaagtcaca gggcgaagaa
     2161 aaaatccggc tgcaaaaggt actgcgcaag gcgggcgatt gcctgggact gctgaatcag
     2221 gattccgaaa gctggttcaa gtggcagccg gccggcaaaa ccggcggttt gagcgacgct
     2281 gaaatcgacg ccctgattgt ggaacgcaac gacgcgaaaa aagcaaaaaa cttcggtcgc
     2341 gccgatgaaa tccgcaagca actgaaggaa cagggcatcg tgctggaaga ttcaaaagac
     2401 ggcacgcgct ggacgcggga ataatcaaaa ggccctgatc aggccggatt cccaccgtcc
     2461 cgcttgccac cgcgctggga tgcctgcagc agcacgtctt cgatcagttt ttccggatcg
     2521 tctgccaacg tggtgaactg ctcttccagc agcagatgac tcaggccatg caccagcgcc
     2581 cacgcgtgca ccatgccgtt gacgacgcgc gggtccgctg catcgcgctt gctggcggca
     2641 gcaacggatg cgagcagcgg ctcaatcgag cgcttctccg cctgctccag ctcgtccgtg
     2701 cgatgggatt tggcgaaacg gccgaacatc agccggaaca gcgccggatt gtcgcgggcg
     2761 aagtgaatat aggcgcggcc tgcaatcaac atcgactcca ccgcatcttt cgcgccttcc
     2821 cgtgcagcca cctgggtcac ccccagacgg cggaacccct ccgccgccag cgccatcagc
     2881 agcgcatcct tgtcggcgaa atggcgatac accgcattgg ccgacacccc cgcctggcgg
     2941 gcgatttccc gcaggctcaa actatcagcc ggggtcgatt ccagcagttc cagaccagcc
     3001 gccaccagcg cctcccgcag gttgccgtgg tggtaggttg tcttcagcgc cggtttggcg
     3061 tcggcaacag cggactgcga cgtcggatca gacttcatgt tgacaccgtg aacattcaag
     3121 gattagcatg ttgacattgt aaacattgga tcatttgtcc gccagaggag aaccccatgc
     3181 aatccaccat cgcccccgtc aatgaatctc tggctatctg gcacgagatg atccgcagcg
     3241 gcgacctgag ccggctgcca gacctgctgc acccggacgc ccagttccgc tcccccatgg
     3301 cgtacaaacc ctaccgcagc gcccaggccg tggcactcat cctcaccacc gtggcccggg
     3361 tgttcgagga tttccattac caccgcgaat tcatcagttc caacggactc gacgtcgtgc
     3421 tggaattcag cgcccgagtt ggcgacaaag ggctgaaagg tatcgacatg atccagttcg
     3481 acgccgacgg ccggatcacc gatttcgaag taatgatccg gcccttcaat gccctgcagg
     3541 cactgggcgg cgccatggca gaacgcctcg gtgccatcct gcccgccttc aaaggccctg
     3601 gcggctgatc gccaacgcaa gaccaatcca acaaggtcaa cccaacaagg agagtcccat
     3661 gagcctgccc cgcctgctcg atgatcttgc agtcaacacc gttgaatgga ccgcccggca
     3721 gatccggaaa caggtgccct actcaccgtc caatccattt ctggaagggc cttatgcgcc
     3781 ggtgcgggaa gaacgcacag aagcggcact gcgcgtcacc ggccacatcc cgccggaact
     3841 caatggtctg tatgcgcgca tcggacccaa tccgatgcag gttgaaaacc ccgccaccta
     3901 ccactggttc accggcgacg gcatg
//
LOCUS       DASUEG010000531         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1689740, whole genome shotgun sequence.
ACCESSION   DASUEG010000531 DASUEG010000000
VERSION     DASUEG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689740"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFX11_10005"
     CDS             <1..180
                     /locus_tag="VFX11_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4938999.1"
                     /translation="SCCNADASAPPASCNQGAIKHTAQKPQAAKIFCSCAAGALMERN
                     QVRKVALCAGRTLPE"
     gene            308..1705
                     /locus_tag="VFX11_10010"
     CDS             308..1705
                     /locus_tag="VFX11_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX4939000.1"
                     /translation="MPLDKYRHWVSAIQSSHKRPAYLMIADLIEADIEKGFLQARDRL
                     PPLRELADLLSLNYTTVTRAYREASNRGLIDSHPGSGSYVKGKTSMLPLRNGSNVEMT
                     MNLPPEIEDAGLLQRIRDDASNVLQGKGLAALLRYQDFGGGPADKEAGALMLGELIPR
                     PDGERILVCPGIHSALVALLSLLCGKKGTLCVGQLVYPGLKAIAAQIGVQLMAVECDQ
                     DGPRISAIEALCKSKQIQALYVNPTIQNPTTFSMSQRRREAIADLALRYSLPIIEDDA
                     YGWVPERAVSPIATRAPELTWYLNGLSKCFGAGCRTAYLYAPNKTQAQRAAGALRALS
                     VMASPITSTLATRWIQNGTVNRVREAQRLQSRERQRLARTILASHHPVANPDGFHLWL
                     PLPRTLAMNPSDVAVFLRNHGISAVAAAAFCTDNNPPAALRLCLGGPVSLPELEQRLI
                     LLQDVLQETVNVTMI"
     gene            1803..2390
                     /gene="smrA"
                     /locus_tag="VFX11_10015"
     CDS             1803..2390
                     /gene="smrA"
                     /locus_tag="VFX11_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HEX4939001.1"
                     /translation="MSPDEDDLFQQEMAGVKPLPKKPVVVTPKPSEPTEAQLARREAA
                     VADLKLDPNTLSTEYVEMVGPHDILEYKRNGVQEGVYRKLRLGKYASEAVLDLHRKTL
                     QQARKDVFEFIQDSTRFGLRTIMILHGKGERSDPPALLKSYVNKWLPSLPQVMAFHSA
                     QKQDGGAGAVYVLLQKNETRKQQNREHYLRHARGR"
     gene            complement(2412..5348)
                     /locus_tag="VFX11_10020"
     CDS             complement(2412..5348)
                     /locus_tag="VFX11_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4939002.1"
                     /translation="MKPLFTLILHIGRRLPRLVGVLAGGCLLLANSFAVNGMDAVNID
                     SGFTRQGIGQPLEYTFDTTGRRTLAEIQNRSQLWHPMDREDLSFGFNPTPLWLRFDVT
                     NTADITRQLLLEISYPVLDQVDVHVLQPGRPVRSVAMGDALPFAARPLQHHHFLAPLT
                     LEPDSTATLYLRVQTEGSLQVPITLWDQTTFLQQQYHFTTWQGLFFGILLIVAVYNLF
                     VFVSLRDAVYLWYALFAVFLGITVGSLNGMAFLYLWPGLPQLNQIAVPLAIALMGLFR
                     NAFTMNLLQLRDNFTRLYRLYVGIVIGYGLLILFVLLAPYHRAITVTAAMALITSVIG
                     MVSGLYLALQGQRTAQIFTCSWAGLLAGVVLVVLNKSGIIASGWWVANSLQLGSILQI
                     LLLSFALSNRFREEREARETAQQRALLNEQRYLELEYSTRMDELDARQKVLKAEADSR
                     AKSEFLATMSHEIRTPMNGVLGMTELLQDTDLQEQQRQYLDVIASSGKALLNIINDIL
                     DYSKIAAGKMELEQIDFDLDKLCLDCASVFSITAERKRLELLCSLEPGTPLFIRSDPT
                     RLRQILLNLLGNAFKFTNEGLVSLRVREIAEPGLPAGQHLLRFEVRDTGTGISEKALE
                     NLFQAFTQADASTSRQFGGTGLGLSISKRLTELMGGEIGVSSQLNLGSTFWFSIRCER
                     AGADFVAENREPADTLQGRRMLIVDDSPEYALMVKEQAESWGMRAQVAYFGSQALELL
                     SDAVETGDPFELVTLDMFMPGWSGLECAQQIQAHPKLKDCLCILLTAMRNIPPKPELQ
                     QAGIRLAIQKPASARALRQALLAVLVDQVQRPFPRRDVHANPLRGKRVLVAEDNTANQ
                     LVITGMLKKLEIDTVLVDNGQKALDHLRNHMGTIDLVLMDCDMPEMDGKEAIRHYRAF
                     EREACLPPLPLIALTAHALPEELGQLRDAGADGVLVKPLTLQELRTALQHYLVPALKK
                     MAPLLS"
BASE COUNT         1089 a         1673 c         1715 g         1063 t
ORIGIN      
        1 tcctgttgca acgccgatgc cagtgcacca ccggcttcat gcaatcaagg tgcgatcaag
       61 cacaccgctc aaaaaccgca ggcagccaaa attttttgca gctgtgctgc aggagcattg
      121 atggaaagga atcaggtacg gaaagtcgca ctgtgcgcgg gcagaacgct gccggaatga
      181 ttcgaaatgt tgcaggatgc gactgaccaa tgatcgcatt ggaatgcaat caatgctgta
      241 gccgcagtgg cgggccatct ggaacggtaa ctgcagggac gtggccaaat gtcgcgagaa
      301 cagcctcatg cccctggata aataccgtca ctgggtcagt gccattcagt caagccacaa
      361 gcgtccggcc tacctgatga ttgcggatct gattgaagcc gatattgaaa aaggttttct
      421 gcaggcgcgg gaccggctgc cgccgctgcg ggaactggcc gatctgctgt cactgaatta
      481 caccaccgtc acccgcgcct atcgggaagc gtccaatcgc ggcctgatcg attcccatcc
      541 cggctcgggt tcctacgtca agggcaagac gtccatgctg ccactgcgca atggcagcaa
      601 tgtggaaatg accatgaatc tgccgccgga aatcgaagat gccggtctgc tgcagcgcat
      661 ccgcgacgat gccagcaatg tgctgcaggg caaggggctg gcggcattgc tgcgctacca
      721 ggattttggt ggcggccctg ctgacaagga agcgggcgcg ctgatgctgg gtgaactgat
      781 tccgcgtccc gatggtgagc gtattctggt gtgccccggc attcacagtg cgctggtggc
      841 attgctctcc ctcctgtgtg gcaagaaggg cacgctctgt gtcggccagc tggtgtatcc
      901 cggcctcaag gccatcgctg cacagattgg tgtgcaactg atggcggtgg aatgcgatca
      961 ggacgggccg cgcatcagcg ccatcgaagc cttgtgcaaa tccaagcaga ttcaggcgtt
     1021 gtacgtgaat cccacgatcc agaatcctac taccttcagc atgtcgcagc ggcgacgcga
     1081 agccattgcc gatctggcgt tgcgatacag cctgcccatc atcgaggacg atgcctatgg
     1141 ctgggtgccg gaacgggcag tgtctcccat cgccacccgc gcgccggagc tgacctggta
     1201 tctgaacggc ctgtcgaaat gttttggtgc cggctgccgc actgcgtatc tgtatgcccc
     1261 caacaagacc caggcccagc gtgcggccgg tgccctgcgg gcgctctcgg tgatggccag
     1321 tccgatcacc agtacactgg ccacccgctg gatccagaat ggcacggtca atcgcgtgcg
     1381 ggaggcgcaa cgcctgcagt cgcgggagcg gcaacggttg gcacggacga tcctggcatc
     1441 gcatcacccg gtggcgaatc ccgacggttt tcacctgtgg ctgccactgc ccagaaccct
     1501 ggccatgaat ccgtccgacg tggcggtgtt tctgcgcaat catggcatca gcgcagtggc
     1561 cgccgcggcc ttctgcaccg acaacaatcc gccggccgca ttgcgcctgt gtctgggtgg
     1621 accggtgagt ttgccggaac tggagcagcg tctgatattg ctgcaggatg tgctgcagga
     1681 aacggtcaat gtcaccatga tctgaacggt ctatttccgt ctattccgct gtgtcgcccc
     1741 cttgaccgga gcgctggccg gccccatctt gtcagcatcg attccccaac aagcacagca
     1801 ccatgagccc tgacgaagac gacctgttcc agcaggaaat ggccggtgtg aagccgctgc
     1861 cgaagaagcc ggtggtggtg acgccgaagc cgtccgaacc cacggaagcc cagctggccc
     1921 gccgcgaagc cgcggtggcc gatctcaaac tcgaccccaa caccctgtcc accgaatatg
     1981 tggagatggt gggcccccac gatattctgg aatacaagcg caatggtgtg caggaaggcg
     2041 tgtaccgcaa gctgcggctg ggcaagtacg ccagtgaagc cgtgctggac ctgcaccgca
     2101 aaaccctgca gcaggcgcgc aaggatgtgt tcgaatttat ccaggacagt acccgtttcg
     2161 gtctgcgtac catcatgatt ctgcatggca agggcgagcg tagcgatccg ccggcgctgc
     2221 tgaaaagtta cgtcaacaaa tggctgccca gcctgcccca ggtgatggct tttcacagtg
     2281 cacagaaaca ggatggcggc gccggcgcgg tgtatgtgct gctgcagaaa aatgaaaccc
     2341 gcaagcagca gaaccgcgag cattatctgc gacacgcgcg ggggcgatga gaagcagcca
     2401 cgggcggcgg ttcaggacag caggggcgcc atcttcttca gtgccggcac cagatagtgc
     2461 tgcagcgcgg tgcgcagttc ctgcagggtc agcggtttca ccagcacgcc gtcggcgccg
     2521 gcgtcccgca actggcccag ttcctccggc agggcatgcg ccgtcagcgc gatcaagggc
     2581 agcggaggca ggcaggcttc ccgttcaaag gcgcggtagt ggcggatggc ctccttgccg
     2641 tccatttccg gcatgtcgca atccatcagc accagatcaa tcgtgcccat gtggttgcgc
     2701 aggtgatcca gcgctttctg gccgttatcc accagcaccg tgtcaatctc cagttttttc
     2761 agcataccgg taatcaccag ctggttggcg gtattgtctt cggccaccag cacgcgcttg
     2821 ccccgcagcg gattggcgtg cacgtcccgg cgcgggaagg ggcgctggac ctgatcgacc
     2881 agcactgcca gcagggcctg gcgcagcgca cgcgctgacg ccggcttctg tatcgccagc
     2941 cggatgccgg cctgctgcaa ttccggtttc ggcggaatgt tgcgcatggc ggtgagcaaa
     3001 atgcacaggc agtctttcag tttgggatgg gcctggatct gttgcgcgca ttccaggccg
     3061 gaccagcccg gcatgaacat gtccagcgtc accagctcga acggatcgcc ggtttcgaca
     3121 gcatcgctca gcagttccag tgcctggctg ccgaagtagg ccacctgcgc gcgcatgccc
     3181 caggattccg cctgttcctt caccatcagt gcatactcgg gggaatcgtc gacgatcagc
     3241 atccggcgcc cttgcagcgt gtccgccggt tcccggtttt ctgccacaaa gtccgctccg
     3301 gcccgctcgc agcggatgct gaaccagaac gtgctgccca ggttcagctg gctgctgacg
     3361 ccgatctcgc cgcccatcag ttctgtcagt cgtttcgaaa tgctcaggcc cagcccggtg
     3421 cccccaaact ggcgactggt ggacgcgtct gcctgcgtga acgcctgaaa cagattttcc
     3481 agcgcttttt cgctgatgcc ggtgccggta tcgcgcacct cgaaacgtaa caggtgctgg
     3541 ccggccggga ggcccggttc tgcaatttcg cgtacgcgca ggctcaccag accttcgttg
     3601 gtgaacttga aggcattgcc cagcagattc agcaggatct ggcgcaggcg ggtgggatca
     3661 ctgcgaatga acagcggcgt gcccggctcc agcgagcaca gcagttccag ccgcttgcgt
     3721 tcggcggtga tggaaaatac cgaggcgcaa tccaggcaga gtttgtccag gtcaaaatcg
     3781 atctgctcca gttccatttt gccggcggcg attttcgaat aatccagaat gtcattgatg
     3841 atgttgagca gtgccttgcc ggaactggcg atcacatcca gatattgccg ctgctgttcc
     3901 tgcaggtcgg tgtcctgcag cagttcggtc atgcccagca cgccgttcat gggggtgcgg
     3961 atttcgtggc tcatggtggc gagaaattcg cttttggcgc ggctgtccgc ttctgccttc
     4021 agcactttct ggcgcgcgtc cagttcgtcc atccgggtgc tgtattccag ttccagatag
     4081 cgctgttcgt tcagcagcgc ccgctgttgg gctgtttccc gcgcttcccg ttcttcccgg
     4141 aagcggttgg aaagggcaaa cgacagtagc aggatttgca ggatcgaacc cagctgcagc
     4201 gagttcgcca cccaccagcc ggacgcgatg atgccggatt tgttcagcac caccagcacc
     4261 actcctgcca gcaggccggc ccaggagcag gtaaagatct gggcggtgcg ctggccctgc
     4321 agcgccagat acaaccccga caccatgccg atgacactgg taatcagtgc catggccgcg
     4381 gtaaccgtga tggcgcggtg atagggcgcc agcagtacga acagaatgag caggccatag
     4441 ccgatcacga tgccgacata caggcggtaa aggcgcgtga agttgtcgcg caactgcagc
     4501 aggttcatgg tgaaggcgtt gcggaacagg cccatcaagg cgatcgccag aggcacggcg
     4561 atctggttca gctgcggcag gccgggccac aggtacagga acgccatgcc attcaggctt
     4621 cccaccgtaa tgcccaggaa aaccgcgaac aacgcgtacc acaggtagac ggcatcgcgc
     4681 aggctgacaa acacgaacag gttgtacacg gccacgatca gcaggatgcc gaaaaacagt
     4741 ccctgccagg tggtgaagtg gtattgctgc tgcaagaacg tcgtctggtc ccacagggtg
     4801 atgggcacct gcagtgagcc ttccgtctgc acccgcaggt acagggtggc ggtgctgtcg
     4861 ggttccagtg tcaatggcgc cagaaagtgg tggtgttgca gcgggcgcgc ggcaaagggt
     4921 aacgcgtcgc ccatggccac ggaccggacc ggccgccccg gctgcagtac atgaacgtcc
     4981 acctggtcca gtaccgggta gctgatttcc agcagcagct ggcgggtaat gtcagccgta
     5041 ttggtcacat cgaagcgtag ccacaggggc gtggggttga agccgaacga caggtcctcc
     5101 cggtccatgg gatgccacag ctgggacctg ttctggatct ccgccagcgt gcggcggccc
     5161 gtagtgtcga aggtgtactc gagcggctgg ccgatcccct gccgggtgaa gccggaatcg
     5221 atgttcaccg cgtccatccc attgaccgca aacgagtttg cgagcagcag acagccaccg
     5281 gcaagcacac ccaccagacg cggcaagcga cgcccgatgt gcaggattag cgtgaatagg
     5341 ggtttcacgg ctgcactctt ttggtcaagg agtggtcaat tatagatcat ggtgaccaaa
     5401 atgcctgaat cgttcctgct gccggcccta agctctggca accgtcttgt ggtcgggcgc
     5461 cagccgttcc agcaactttt ccttgagggt ggtgaattcc agcggtttgc cgatatggtc
     5521 gtccatgccg gcctgctggg
//
LOCUS       DASUEG010000532         5580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_532587, whole genome shotgun sequence.
ACCESSION   DASUEG010000532 DASUEG010000000
VERSION     DASUEG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532587"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFX11_10025"
     CDS             <1..990
                     /locus_tag="VFX11_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX4939003.1"
                     /translation="DDIPKKGFILRTAAEAADEESLRRDVDFLCRLWRAISERMLKSN
                     PVTLIHRDLPLAMRTLRDEVRPEVEKIRIDSRETHGKVIEFVREYIPDIEPLIEHYPG
                     ERPIFDLYSVEQEVQKALARQVQLKSGGHIVIDQTEAMATIDVNTGAYVGHRNLEETI
                     FKTNLEATQAIARQLRLRNLGGIIIIDFIDMEDAEHKRQVLRIFEKALERDHAKTKIT
                     QVSELGLVEMTRKRTRESLARVLCEECPTCSGRGIVKTPETVCYEIFREILRQFRAYD
                     AEGFLVIASQAVVDRLLDEESANVSDLELFIGKTIRFQVEAQYSQEQFDVVLM"
     gene            1003..5028
                     /locus_tag="VFX11_10030"
     CDS             1003..5028
                     /locus_tag="VFX11_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX4939004.1"
                     /translation="MVKRVATGLVKWSIGLAVALLVVLAVLVGLARQVVYEIDSLQAD
                     VAQYLSNRTGVVIDFAHVSGSWKGLAPRFRIEQLSVRRSPEHEPAITARHADLEVLLL
                     QSALLLQPRVRLTVDGLVLTLAKRDGRMVVEGLDAPPASDRPATDFNALADLIAAQPR
                     LAINDSLMTLKGLYAQDVALSVQNLQIESGKVRRYAKGRVTLKGPSNLSFDLKAQMRG
                     SLLRAGSLTGDLYLDAGFADWLPWIPPEHRQFKTATLESLQGGSESWLKLKNGRVVDA
                     TSRFTLHDFNLASGNEVQPPRIKTLSGVGRWQEASPGNWTLALHDLRMVTPSFVWQPS
                     ELRVSATALGADDNRFAIALDDADITPWLNYFLALQPEEGTLYRTLKELRPAGKLHQL
                     ALAVEQKAGAITDYRFSADIRQFNSRPWEKYPGFRDLDINLKGRQGKLLVKLADDYLE
                     LNYPWLFRDVLSLTRLDGSLLLNRTDAGFELQSSLLRVHSSDLRSATQFSLLLPADTT
                     QPPFLALQSTLRDVDAEKKSLYLPFGVIPPKLLQWLDEGILSGRLARGDIVVHGALGK
                     DHASDRRLLLGFTVHDAALRFLPDWTEPVKNLDADVIVDRGAVYGEAVQGEYFQQVLQ
                     SARVTVPPVPQGAQHLLQVQALTAGRADTGFDVLQTTPLAKLAGPIIQDMEVQGDMTV
                     DLDLAVPLDKGPRKTEVDVDVALRNGTFDLTSQKLQAVDLNTDVHFDLEHGLSAPALR
                     GQSFAGPLTGKLETRKVRQGGQTIVLGLQGKAQVSALQDWLHLSLLQPLSGALDYQVD
                     LQVPTGEAKKHLHGYLNVTSQLKGTRIDLPAPYGKSAKARREFRVWQSMDREPSLFSV
                     RYDDLFELSLQRRQGQVSKGVLTLGGQKAVLPSDDVFRVEGNLGRFDPAEWETALAGI
                     RKASGQYQENVDSTRLALLDSSRLTINELILGENNLGALSIDLQRQGGDWRLGLSSNA
                     IAGTVQLPPHVFKGAGAFTAADRPVQVQLDHVRLPKKELPEGAPEPEWVPSSISPLDL
                     PNARVLIKDLQVGDGSFGRWALSLQRTPQGLQLNDIDVFMRSVQLKAKGDWTDIGGVR
                     TTKVLGSFTSSNIADVMRDWGTEPSMSSHKMTGNLNLNWPGAPFEFSLRRAGGAFDVD
                     LQKGAFLNVKSGVVGKFWGALNFETLLRRLQLDFKDLSEKDMVYDRIQASANLDRGLM
                     HVKDVSLDSPAIKLQTSGDINLHTDQLDMVMRVTVPVTRNLVIPAAAIGGVPAGAAAF
                     VIERMLGDQFDKLTTLKYSVKGGFDDPKVEIVNSFNLIPSQVRDAVMGSDKAPAPGTP
                     SPAVPLSPAVPPADNSGATRP"
     gene            5025..>5580
                     /locus_tag="VFX11_10035"
     CDS             5025..>5580
                     /locus_tag="VFX11_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX4939005.1"
                     /translation="MKTIVAALQMVSTFDVDANLKSAEQLLRQAADQGAQFAALPENF
                     AVLDSDNLCRWGDEETRTGVFTTFLAEQARKCNLWILGGTIPKRTLADGKPVPGWRVR
                     AASILVNPQGDIVARYDKMHLFDVQVSDAQGQYRESATIEPGNVPVLATTPFGKLGMT
                     VCYDLRFPELFSLLQRQGADFFTVP"
BASE COUNT         1090 a         1614 c         1809 g         1067 t
ORIGIN      
        1 gacgacattc ccaagaaagg ttttatcctg cgtaccgccg cggaggctgc cgacgaggaa
       61 tccctgcgcc gtgacgtgga ctttctctgc cgcctgtggc gcgccatcag cgagcgcatg
      121 ctgaagtcca atccggtgac actgatccac cgcgatctgc cgctggccat gcgcaccctg
      181 cgtgatgaag tgcggccgga agtggaaaaa atccgcatcg attcgcgcga gacccacggc
      241 aaggtgatcg agttcgtgcg tgaatatatt cccgacatcg agcccctgat cgagcactat
      301 cccggcgagc gccccatctt cgatctgtac agtgtcgagc aggaagtgca gaaggcgctg
      361 gcgcggcagg tgcagctgaa gtccggcggc catatcgtga ttgaccagac cgaagcgatg
      421 gcgacgatcg acgtcaacac cggcgcctac gtgggccacc gcaatctgga agaaaccatt
      481 ttcaaaacca atctggaagc gacgcaggcc attgcgcggc agctgcgtct gcgcaatctg
      541 ggcggcatca tcatcattga cttcatcgac atggaagatg ccgagcacaa gcgccaggtg
      601 ctgcggattt ttgaaaaggc cctggagcgc gatcacgcca agaccaagat cacgcaggtg
      661 tcggaactgg ggctggtgga gatgacacgc aagcgcacgc gggaaagtct ggcgcgggtg
      721 ctgtgcgagg agtgccccac ctgcagcggc cgcggcattg tgaaaacacc cgagaccgtg
      781 tgctacgaaa tcttccgcga aatcctgcgt cagttccgtg cttatgacgc cgaaggtttt
      841 ctggtgattg cgtcgcaggc ggtggtggat cgtctgctgg acgaggaatc cgccaacgtg
      901 tccgatcttg aactcttcat cggcaagacc atccgctttc aggtggaagc gcaatattcc
      961 caggagcagt tcgatgtggt gctcatgtaa gggatggcgg cagtggtaaa gcgggttgcc
     1021 acggggctgg tgaaatggtc gatcggactg gcagtggcgc tgctggttgt gctggccgta
     1081 ctggtggggc tggcgcggca ggtggtgtac gaaatcgaca gtctgcaggc ggatgtggcg
     1141 cagtatctgt cgaaccggac cggagtggtg atcgatttcg cccatgtcag tggcagctgg
     1201 aagggactgg cgccgcgttt ccggattgag cagttgagcg tgcggcgctc gcctgagcat
     1261 gaaccggcga tcaccgcccg acatgcggat ctggaagtgt tgctgttgca atcggcgttg
     1321 ttgctgcagc cccgggtgcg gctgacggtg gatggtcttg tgcttaccct ggcaaagcgt
     1381 gacggccgca tggtggtgga agggctggac gcgccgccgg ccagcgacag gcccgccacc
     1441 gatttcaacg ccctggcgga cctgattgcg gcgcaacccc gattggcgat caatgacagc
     1501 ctgatgacgc tgaaggggct gtatgcgcag gacgtcgccc tgtcggtgca gaatctgcag
     1561 atcgaatccg gcaaggtgcg gcgctatgcc aaaggtcgtg tcacgctcaa ggggcccagc
     1621 aatctttcgt tcgatctgaa ggcacagatg cgcggcagtc tgctgcgggc tggttcgctc
     1681 accggtgatc tgtatctgga tgccggcttt gccgactggc tgccctggat tccaccggaa
     1741 caccggcagt tcaaaaccgc cacgctggag agcctgcagg gcggcagcga gagctggctg
     1801 aaactcaaaa acggccgtgt ggtggacgcc accagtcgat tcaccctgca cgatttcaac
     1861 ctggccagcg gcaacgaagt gcagccgccg cgcatcaaaa cgctgtcggg ggttggccgc
     1921 tggcaggagg caagccccgg caactggacc ctggcactcc acgatctgcg catggtcacg
     1981 cccagtttcg tctggcagcc cagcgaactg cgcgtgtcgg ccacggcact gggcgcggat
     2041 gacaaccgct tcgccatcgc gctggacgat gccgacatca cgccctggct gaactatttt
     2101 ctggcgctgc aaccggagga aggcacgctc tatcgcacgt tgaaggagct gcgtcctgcc
     2161 ggcaagctgc accagctggc gttggcggtg gagcagaaag cgggcgccat tactgactat
     2221 cgtttcagtg ccgatattcg ccagttcaac agccgtccct gggaaaaata cccgggtttt
     2281 cgcgatctgg acatcaatct gaaaggccgc cagggcaagt tgctggtgaa gctggcggat
     2341 gattacctgg agctgaacta tccctggctg ttccgcgatg tgctgtccct cacccggctt
     2401 gatggcagtc tgctgctgaa ccgcaccgac gccgggtttg aactgcaaag cagcctgttg
     2461 cgggtgcaca gcagcgatct gcgcagtgcc acccagtttt ccctgctgtt gccggccgat
     2521 accacccagc cgccgttcct cgcgctgcag tccaccctgc gcgatgtcga tgccgagaaa
     2581 aaatccctgt acctgccgtt tggtgtcatt ccaccgaaac tgctgcagtg gctcgacgaa
     2641 ggcattctgt cgggacggct ggcgcggggt gacattgtcg tgcacggcgc actgggcaag
     2701 gaccatgcct cggaccgccg cctgctgctg ggcttcactg tgcatgatgc cgcgctgcgt
     2761 ttcctgccgg actggaccga accggttaaa aatctggatg ccgatgtaat cgtggaccgc
     2821 ggcgcggtgt atggcgaagc ggtgcagggc gagtatttcc agcaggtatt gcagtccgcg
     2881 cgggtgaccg tgccaccggt accgcaggga gcgcagcatc tgctgcaggt tcaggcgctg
     2941 actgcgggac gtgccgacac cggttttgac gtactgcaga ccactcccct ggccaaactt
     3001 gccggaccca tcatccagga catggaggtt cagggtgaca tgactgtgga tcttgacctg
     3061 gcagtgccgc tcgacaaggg cccgcgcaaa accgaagtcg acgtggatgt cgcgctgcgt
     3121 aatggtacct tcgatctcac cagccagaag ttgcaggcag tggatctgaa caccgatgtg
     3181 cacttcgatc tggaacatgg tttgtcggcg ccggcgctgc gcgggcagtc gttcgcaggt
     3241 ccgctgaccg gcaagctgga aacgcgcaag gtccggcagg gcggccagac catcgtgctg
     3301 ggattgcagg gcaaggccca ggtgagcgcg ctgcaggact ggctgcacct gtcgctgctg
     3361 cagccgctgt ccggcgcact ggattatcag gtggacctgc aggtgcccac cggcgaagcg
     3421 aaaaaacacc tgcacggcta tctgaatgtt acctcgcaac tgaaaggcac ccgcatcgat
     3481 ctgccggcgc cctatggcaa gagcgccaag gcgcgccgcg aattccgcgt ctggcagtcg
     3541 atggatcgcg agccgtccct gttcagcgtg cgctacgacg atctgtttga actgtcgctg
     3601 cagcgacgcc agggccaggt gagcaagggt gtgctgacac tcggtgggca gaaagccgtg
     3661 ttgccgtctg acgatgtgtt ccgggtggaa ggcaacctgg ggcggttcga tccggcagaa
     3721 tgggaaaccg cactggcggg cattcgcaag gcgtccgggc agtatcagga aaacgtcgat
     3781 tccactcgcc tggcattgct ggattcgtca cggctgacca tcaatgagct gattctgggt
     3841 gaaaataacc tcggcgcgtt gagtatcgat ctgcagcgac agggcgggga ttggcgcctg
     3901 ggcctgagca gtaatgccat cgccggtacg gtgcaactgc cgccgcacgt tttcaagggg
     3961 gcgggcgcct tcacggcggc agaccggccg gtgcaggtgc agctggatca tgtgcggttg
     4021 ccgaaaaagg aattgccgga aggtgcaccg gaaccggaat gggtgcccag cagcatttca
     4081 cccctggatc tgcccaatgc ccgcgttttg ataaaggacc tgcaggtggg cgatggcagc
     4141 tttgggcgct gggcactgag tttgcagcgc acgccgcagg gtctgcaact gaatgatatc
     4201 gacgtgttca tgcgcagcgt gcagctgaaa gccaaaggcg actggaccga tatcgggggt
     4261 gtgcgcacga cgaaagtgct gggcagtttc acgtccagca acattgccga cgtgatgcgt
     4321 gactggggca ccgaaccttc catgagtagc cacaaaatga ccggcaatct caacctgaac
     4381 tggcccggag ctccgtttga gttttctttg cggcgtgctg gtggcgcgtt cgacgtcgat
     4441 ctgcagaaag gtgccttcct caacgtcaag tccggtgtgg tgggcaaatt ctggggcgcg
     4501 ctcaatttcg aaacactgct gcgtcggctg cagctggatt tcaaggatct gagtgagaag
     4561 gacatggtgt atgaccgcat ccaggccagc gccaatcttg atcgcggact gatgcatgtg
     4621 aaggacgtca gcctcgacag ccctgccatc aaactgcaga ccagcggcga tatcaatctg
     4681 cacaccgatc agctggacat ggtgatgcgg gtgacggtgc cggtgacgcg caatctggtg
     4741 attccggcag cggctatcgg cggtgtgccg gcaggtgccg ccgctttcgt gatcgagcgc
     4801 atgctgggcg accagttcga caagctcacc accctcaaat acagtgtgaa gggtggattc
     4861 gacgatccca aggtggaaat cgtgaactcg ttcaatctga ttcccagcca ggtgcgcgac
     4921 gcagtgatgg gcagtgacaa ggcgccggca ccgggcaccc cgtcgccggc agtgccgcta
     4981 tcccccgctg taccacccgc tgacaattcc ggagcaaccc gcccatgaaa accattgttg
     5041 ccgcgctgca gatggtcagc actttcgatg tcgacgccaa tctgaaatcc gccgaacagt
     5101 tattgcgaca ggcagcggac cagggtgcgc agtttgccgc gctgccggaa aattttgctg
     5161 tgctggacag cgacaacctg tgccgctggg gcgatgagga aacccgcacg ggcgtgttca
     5221 ccacatttct ggcggagcag gcgcggaaat gtaacctctg gattctgggc ggcaccattc
     5281 ccaagcgcac gctggcggac ggcaagcctg tgccaggctg gcgcgtgcgt gcagcatcca
     5341 ttctggtgaa tccgcagggt gatatcgtgg cgcgctacga caagatgcac ctgttcgatg
     5401 tgcaggtcag tgacgcgcag ggccaatacc gcgaatctgc caccattgag ccgggcaatg
     5461 tgcccgtgct ggcgaccacg ccattcggca agctcggcat gaccgtctgc tacgatctgc
     5521 gttttcccga actgttcagc ctgctgcagc ggcagggcgc ggattttttc acggtgccgt
//
LOCUS       DASUEG010000533         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2201960, whole genome shotgun sequence.
ACCESSION   DASUEG010000533 DASUEG010000000
VERSION     DASUEG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201960"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VFX11_10040"
     CDS             complement(<1..1254)
                     /locus_tag="VFX11_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4939006.1"
                     /translation="MGKRKLVVVGNGMAGVRTVEELLKLAPELYDITIFGAEPHVNYN
                     RILLSPVLCGEKTPEEIVLNDAAWYAENGITLLTGKAVTEVDRHRRCVRAADGTEVPY
                     DRLLLATGSDPFIIPLPGKDLPGVVTFRDFKDVDAMLEAARSGQRHAVVIGGGLLGLE
                     AANGLLKRGMDVTVIHLTDTLMERQLDRPAGALLQKSLEERGLKFRMGAQTAAILGES
                     RVTGVRFKDDSEIPADLVVMAVGIRPNVTLAKQMGLHCERGVVVSDTLQTFDPAIYAV
                     GECAQHRGIAYGLVAPLYEQAKVCANHLAGLGYGRYTGSVTATKLKVTGIDLFSAGDF
                     SGGEGTQDIVFQDPRRGVYKRLVLDGENRIKGSVLYGDTGDGSWYFQLLRDRTDVSEM
                     REMLLFGQAHLGDSGRGGSSNAANLP"
     gene            complement(1738..>2168)
                     /locus_tag="VFX11_10045"
     CDS             complement(1738..>2168)
                     /locus_tag="VFX11_10045"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX4939007.1"
                     /translation="IVFPEIQINDVGAGDVIGGDNNAGEVNVLQPGILPVAFSVVYTF
                     APEALISPYIGLGPLIGNFRNGRAWTDSGDLLKLQGSTSLGVVGKMGALLTVSQSSYI
                     FIEGRYGYIDSPEVRDRQGDKVDVESFDMLSLKGGLGFNF"
BASE COUNT          445 a          684 c          606 g          433 t
ORIGIN      
        1 gggcagattg gcggcattgg aactgccccc ccgccctgaa tcacccagat gggcctggcc
       61 gaacagcagc atttcgcgca tctcggacac atccgtgcga tcccgtagca gctggaaata
      121 ccaactgcca tcgccggtat cgccatagag aacactgcct ttgatgcggt tctccccatc
      181 cagcaccagg cgtttgtaga ccccccgccg gggatcctga aacacgatgt cctgggtccc
      241 ctcgccgccg gaaaaatccc cggcggagaa cagatcgatg ccggtgacct tgagcttggt
      301 ggccgtcacc gagccggtgt agcggccata acccaagccc gccaggtggt tggcgcacac
      361 cttggcctgt tcgtagagcg gcgccaccag gccgtaggcg atgccccgat gttgcgcgca
      421 ctcccccacc gcatagattg cgggatcaaa ggtttgcagg gtatcgctga ccaccacgcc
      481 gcgctcgcaa tgcaggccca tctgcttggc cagcgtcaca ttggggcgga tgcccacggc
      541 catcaccacg agatcagcgg gaatctcgct gtcatccttg aagcggacgc cagtcacgcg
      601 cgattccccc agaatggctg ccgtctgggc acccatgcgg aacttgaggc cccgttcttc
      661 cagggatttc tgcagcagcg cgccggctgg gcgatccagt tgtcgttcca taagcgtgtc
      721 agtcagatgg atgaccgtga cgtccatgcc gcgcttgagc aggccattgg cggcttccag
      781 cccgagcagg ccgccgccga tgaccacggc atggcgctgt ccggagcggg ccgcctccag
      841 catcgcgtcc acatccttga aatcacggaa ggtcacgacc ccgggcagat ccttgcccgg
      901 cagcggaatg atgaagggat ccgagccggt ggcaagcagc agacggtcgt aggggacctc
      961 agtgccatcg gcggcccgga cgcagcggcg gtggcggtcc acttcggtca ccgccttgcc
     1021 ggtcagcagg gtaatgccgt tctcggcgta ccaggctgca tcgttgagga cgatctcctc
     1081 cggcgtcttt tccccgcaca ggacggggga cagcaggatg cggttgtagt tgacgtgggg
     1141 ctccgcgccg aagatggtga tgtcgtacag ttcgggagcc agcttgagca gctcctcgac
     1201 tgtccgcacc ccggccatgc cattgcccac caccaccagt ttccgcttgc ccatgccgtt
     1261 ttgcctcccg agcctgtcgt ccaagcctcg aagcaatatt tgcgccaaaa gaaaaatcgt
     1321 ctttcaatca gtcgcctgca ccttggttta cttgtcgcat ggcagaataa tcgccattat
     1381 gcacatcaag tgatcattct cttgtccgac tgcatcatga cggcacattt acgggctttc
     1441 cgattcaggc aaatcgatag ctcaataact tgcgtcaaat attggcctgt tctgcagtgg
     1501 cctctcatgt taaagacgaa agattcgcac agttatggtt cgcgttgaac cacaagcgtg
     1561 cgctttttgg catctggatc gctgtattgg cggtcaagcg acctaaaata ggtcattcca
     1621 tctggccgtg ttttcccgct tccagagtgc agccagcctc acttgcaaac ccaagaacca
     1681 cgcataaaaa aacccgactc tatggaagag ccgggttaat ccaagacgtc agcgatctta
     1741 gaaattgaag cctaggccac ccttcaggct gagcatgtcg aaactctcga catcaacctt
     1801 gtcgccctgg cgatcccgca cttcggggct gtcgatgtag ccgtagcggc cctcgataaa
     1861 aatataggac gattgggaaa cggtgagcag ggcgcccatc ttgcccacca cgcccaggct
     1921 ggtggaaccc tgcagtttca gcagatcgcc cgaatcggtc catgcccggc cgttgcggaa
     1981 gtttccaatc aatggtccga gacctatgta agggctgatg agcgcctcgg gcgcaaaggt
     2041 atacaccaca ctgaatgcca ccggcaggat gccaggttgc agcacattga cctcacccgc
     2101 gttgttatcg ccaccgatga cgtcacccgc gcccacatca ttgatctgga tttccgggaa
     2161 cacgatgt
//
LOCUS       DASUEG010000534         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_971397, whole genome shotgun sequence.
ACCESSION   DASUEG010000534 DASUEG010000000
VERSION     DASUEG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971397"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFX11_10050"
     CDS             complement(<1..669)
                     /locus_tag="VFX11_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939008.1"
                     /translation="MSGVTVSPKQGSLDVAQGGSLTLKWVVSTSPAHTGGAFSAQGLL
                     TDPVTGAILHTVATPFNRTEGSGPLHFDEVLALTPAQLQEWQAKGISAVSYQRAFGSS
                     ASDTGNKAAAASANLVITLTGVADASAPRTPVAGLFIHKLNLRFANARNKKRFSPGSS
                     LRAKLDLRYSGTGLLQGTWQIASDIGTGTKPVFTTLAQVRKQLKLVQREFLVSPELPT
                     QAPGN"
     gene            complement(852..1745)
                     /locus_tag="VFX11_10055"
     CDS             complement(852..1745)
                     /locus_tag="VFX11_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4939009.1"
                     /translation="MSDPTPLDDEKIQRILRGITVPPQPQIIVDIHMEQAMPNPDINR
                     IGKLISHDVGLAGTILKVVNSSLFGLKNRITSIEQAVRILGLQTIIRITEGLSIKGEM
                     DDETIIALGGFWDSAMDIASVSATIARDIGYHSPDKAYCLGLFHNCGVPLLMRAHADY
                     PDIMRESYSHPGERVIDTENRKLKTNHAVVGYFVAKSWSLPPDLCEVIAEHHSSEDIF
                     RESTRATYDSGKKTLLAILKMAEHICGLHSILGGQAVDHEWNAISRDLLAYVGLSDLD
                     FDDMAARLEHLGISSQETFHD"
     gene            1884..>2204
                     /locus_tag="VFX11_10060"
     CDS             1884..>2204
                     /locus_tag="VFX11_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HEX4939010.1"
                     /translation="MKAKEDRRLKGGHLWIYSNEVDIVASPLKDLEPGCEVLVETAGG
                     QPLGRGYANPHSLICCRLLTRDWRCALNQKLLAERLQEALSLRETVYSKPYYRLVYGD
                     SDGLS"
BASE COUNT          397 a          649 c          718 g          440 t
ORIGIN      
        1 attgcccggc gcctgggtgg gcagttccgg gctcaccaga aattcccgtt gcaccagctt
       61 cagttgcttg cgcacctgcg ccagggtggt gaacaccggt ttggtgccgg tgccgatatc
      121 gctggcaatc tgccaggtgc cctgcaacag acccgttccg gaataacgca gatccagttt
      181 ggcacgcagg gaactgccgg gtgaaaagcg ctttttgttg cgggcattgg caaagcgcag
      241 attgagtttg tggatgaaca ggccggcgac cggggtgcgt ggcgcactgg cgtcggcgac
      301 gccggtgagg gtgatgacca ggttggcgct ggcggcggca gccttgttgc cggtatcgct
      361 ggcactggaa ccgaacgcgc gctggtagga tacggcggaa atgcctttcg cctgccattc
      421 ctgcagctgt gccggagtga gagccagcac ctcgtcgaaa tgcagtggac cggagccctc
      481 ggtacggttg aacggtgtgg ctaccgtgtg caggatggcg ccggtgaccg gatcagtgag
      541 caggccctgg gccgaaaatg cgccgcccgt gtgggccggg ctggtggaga ctacccattt
      601 cagagtaagt gaaccgccct gggcgacatc cagggagccc tgcttcgggc tcacggtgac
      661 gccgctcact gccgcgaggg cctgagagac agcaagcaat aaggaaaaca gtgcgaacaa
      721 aaggttcgcc agacgatgac acgacagcat gaccggtttc agccccaatt acctgatccg
      781 acgtggcggg tagtctaacc acagggctat tgcgtggcaa ttttgctgac aatatgactg
      841 tcgggcgatg gctagtcgtg gaaggtttcc tggctgctga tgcccagatg ttccagcctg
      901 gcggccatgt cgtcgaaatc cagatcgctc aggcccacat aggccagcag gtcacggctg
      961 atggcattcc actcgtgatc caccgcctgc ccgcccagga tcgagtgcag gccgcagatg
     1021 tgttccgcca tcttcaggat cgccagcagg gttttcttgc cgctgtcgta ggtggcgcgg
     1081 gtggattcgc ggaagatgtc ctcactgctg tggtgctcgg cgatcacctc gcacaggtcc
     1141 ggcggcaggc tccaggattt cgccacgaaa tagcccacca ccgcatgatt ggtcttcagc
     1201 ttgcggttct cggtgtcgat cacgcgctcg ccggggtggg agtaggattc gcgcatgatg
     1261 tcggggtagt cggcgtgggc acgcatcagc agcggcaccc cgcaattgtg gaacagaccc
     1321 agacagtagg ccttgtccgg cgagtggtag ccgatgtcgc gggcgatggt ggcggacacc
     1381 gaggcgatgt ccatggcgct gtcccagaag ccgcccaggg caatgatggt ctcatcgtcc
     1441 atctcaccct tgatcgacag gccctcggtg atgcggatga tggtctgcaa ccccaggatg
     1501 cgcaccgcct gctcaatcga ggtgatgcgg tttttcaggc cgaacaggct ggagttcacc
     1561 accttgagaa tggtgccggc cagccccacg tcgtggctga tgagcttgcc gatccggttg
     1621 atatccgggt tcggcatcgc ctgttccatg tgaatgtcga cgatgatctg cggttgcggc
     1681 ggcacggtga tgccgcgcag gatgcgctgg attttctcgt cgtccagtgg ggtggggtcg
     1741 ctcattccgg tatacttgcc cgttcctgaa ttcaagcact gcaattcagt gtagcagccg
     1801 tcccctgcat tgcccttttc cccagccgag tccgcaccgt gtcttcaaat tcgccgcacc
     1861 cagaatcact cccccgtatc cgtttgaaag ccaaggaaga tcgccgcctg aagggtggac
     1921 acctgtggat ctacagcaac gaagtggaca tcgttgcctc gccgctgaaa gacctggagc
     1981 ctggctgcga ggtgctggtg gaaaccgctg gcggccagcc cctgggccgt ggttacgcca
     2041 acccgcactc gctgatctgc tgccgcctgc tgacccgcga ctggcggtgt gcgctgaacc
     2101 agaagctgct ggcggagcgc ctgcaggagg cgctgtccct gcgtgaaact gtgtacagca
     2161 agccctatta ccgcctggtg tacggcgaca gcgacggcct gtcc
//
LOCUS       DASUEG010000535         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_555609, whole genome shotgun sequence.
ACCESSION   DASUEG010000535 DASUEG010000000
VERSION     DASUEG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555609"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..836
                     /gene="narI"
                     /locus_tag="VFX11_10065"
     CDS             147..836
                     /gene="narI"
                     /locus_tag="VFX11_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX4939011.1"
                     /translation="MIMLNKILFGLYPYIAVTVCLVGCWIRFDREQYSWKTGSSQLLR
                     GKNFRIASNLFHVGIIFILMGHIVGLLTPEAIYHHVITTQAKQILAMVSGGFFGILCL
                     VGLLMLIQRRMTDPRIRNTGNTSDIALLLLLLAQLVLGLSTIVASTQHLDGSVMVMLA
                     NWAQSIVLLQPQQAADAIAPVRVVYKLHVLLGMTLILIVPFTRLVHVISAPVWYLGRR
                     YQIVRQKAAQG"
     gene            854..1624
                     /locus_tag="VFX11_10070"
     CDS             854..1624
                     /locus_tag="VFX11_10070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4939012.1"
                     /translation="MHAQTLPVVRINGVEISEQAIAAEMQYHPAATLEQARQAASSAL
                     AVRELLLQEASRQGCDGEPDDMIDQLLAQALNLPQAGEQECRQYYASNPQRFLSPTLL
                     EVRHILLPAAPDDADARQQALEKARALIATLQRNIATFDELARDHSVCPSREQGGSLG
                     QLSKGSTVPEFERQVFAAQPGLMTQPVETRYGYHVVQVERKIPGERLPYENVRDQVRR
                     YLEQKVFYTALSQYLRLLTAAAAIEGVALEAATSPLLQ"
     gene            1755..3053
                     /locus_tag="VFX11_10075"
     CDS             1755..3053
                     /locus_tag="VFX11_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939013.1"
                     /translation="MVQPFSRHYTRHSVLVALVASGVCGGLAFLLLLLGLHPAVAVFF
                     MVSMLLLLAFAAYLHARVFQYSTYLQDILEAAPSGILAIDEQGCVRFANPAARQIFEY
                     EKDELAGLSVEKLIPSSIRDKHPQLRSSFVRERRSRRMGEGRDIHGVTRSGAEVPLEI
                     GLTFLSTGASPMIIVGVIDIRELKAAQTTIVRQNMNLARSVKELEQFAFIASHDLREP
                     LRKVMNYAEIMQEDYGNIIDEDGRTVLKSMSGAVQRMERLLDSLLNYSRITTRAQPFE
                     EVSMMDVIADVVEDLSLSISESGAKVICSGLPAVMGDRNQLRQLFQNLISNSLKYHRD
                     NVAPVIRIESNNRPAPNADPHYVAILVRDNGIGFDNQYSELIFDVFKRLHGRDKYQGT
                     GMGLAICRKIVERHEGFIVASGQVDAGAEFCIWLKRGRVK"
     gene            3066..3647
                     /locus_tag="VFX11_10080"
     CDS             3066..3647
                     /locus_tag="VFX11_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939014.1"
                     /translation="MMPVCEFCTDNVGSVVYGQGLTNTGFNRALSSWAGTGWVMTGMT
                     APSEAAALAAILIVDDDLEDVQFIRRAFKKNNVANPLFSVFSGEEALDFLGRCDRGSQ
                     AYPGLILLDLNMPGVGGMETLRRVKMNERWRRIPAVIFTTSKAEEDVLTSYSMGANSY
                     ISKPFGFDGLTEVVAQISSYWLGVAALPSKSAE"
     gene            3651..5366
                     /locus_tag="VFX11_10085"
     CDS             3651..5366
                     /locus_tag="VFX11_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4939015.1"
                     /translation="MQTQLRAPAVKTPDQNLKLLLVDDDDEDLYFLKRLLKQSGRSHD
                     VLEARNVDQAVEQLQHQPDIVLLDFYLGHQTAFDLLQRMQKSGCQLPVILLTGHDDAE
                     IDQLALNKGIADYLPKRIVNTAILDRSIRYAHRDFQRLQQLEYLAHYDTLTGLCNRRL
                     FMDRLQHAYENARRQNRTGALLYIDVDGFKQINDELGHATGDELLKVVAERMSLVVRS
                     ADTVARFGGDEFVILLENIGAPEAHRVAQKLLYSMEKPVQLRSGPVQCSLSIGLCGFS
                     AEYGSPDEILSSADRALYQAKTIGKHTYCSFNQRLMESLTRQTLIERLAQQAVDSGDF
                     FMHYQPRMSSQGNGVVGFEALARWPRRDDVQLTPGDFIPVLEALGLMDRFTQWVIDKA
                     LADLPRLRQWAPGCVLSLNVSSSSFASDSVKDYLLQKLQQLQIDPSGIEVEVTESLFI
                     ERPESAVRILTELRAVGVRVALDDFGTGYSSLSYLNQLPIDTIKLDRQFLEQVPAEPR
                     STLLVAAIVDLAGNLQLNVVAEGVEREPQKAFLQSCGCHEMQGYLTGRPMSVDQLQAF
                     FSKPD"
     gene            complement(5363..6010)
                     /gene="can"
                     /locus_tag="VFX11_10090"
     CDS             complement(5363..6010)
                     /gene="can"
                     /locus_tag="VFX11_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX4939016.1"
                     /translation="MAKLYELLANNVAWADRVKADDPHFFERLSKTQAPEYLWIGCAD
                     SRVPANEIIGLLPGEVFVHRNIANLVVHTDFNCMSVLEYAVEVLKVKHVLVVGHYGCG
                     GIRAALDGTHHGLIDNWLYNIKNLYYRNKYRMDSIDREEDRLNFLCELNVMQQVKNVA
                     HTHVVQRAWERGQPLNIHGWIYSLRDGLAKDLGVSIQSPEELADEFRVVNGLRDG"
     gene            complement(6109..>6535)
                     /locus_tag="VFX11_10095"
     CDS             complement(6109..>6535)
                     /locus_tag="VFX11_10095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX4939017.1"
                     /translation="ESAEIAYSYAASHIQQYGGDPAFFDKAAIHVHVPEGATPKDGPS
                     AGVTMTTALISLARNRSLPRPLAMTGEITLTGQVLPVGGIREKVIAARRQKIRELILP
                     EANRRDYDELPDYIRKGITVHFADHYQDVYRAVFAPSSL"
BASE COUNT         1304 a         1845 c         1965 g         1421 t
ORIGIN      
        1 tgcccgtcga accgcccgca gcgtccttct gccagccagc ctgtttcaac cgacgctctg
       61 gcggcggtga aatgggtgga agggcaggct gccgcaccgc gcactgcaac ccgttaagta
      121 tgcccgttac ctagaagagt cgggagatga tcatgttgaa taaaattctg tttggacttt
      181 acccctacat cgccgtcacc gtttgtctgg tgggctgctg gattcgtttc gaccgcgagc
      241 agtacagctg gaaaaccggt tccagccagt tgctgcgtgg aaaaaacttc cgcatcgcca
      301 gcaatctgtt tcatgtcggc atcattttca ttctgatggg acacatcgtc ggcctgctga
      361 caccggaagc catttaccat cacgtcatca ccacccaggc caagcagatt ctggcgatgg
      421 tgtctggtgg cttcttcggc attctgtgcc tggtggggct gttgatgctg atccagcgtc
      481 gcatgaccga tccgcgcatc cgcaacaccg gcaacacgtc ggacattgcg ctgctgttgc
      541 tgttgctggc acagctggta ctcggcctca gtaccatcgt ggcgtccacc cagcatctgg
      601 acggctcggt gatggtgatg ctggccaact gggcgcagag cattgtgctc ctgcaacccc
      661 agcaggcggc agacgcgatt gcgccggtgc gtgtagtgta caagctgcac gtgctgctgg
      721 gcatgacact gattctgatc gtgccgttta cccgtctggt acatgtgatc agcgcgcccg
      781 tgtggtatct gggtcgccgt tatcagatcg tccgccagaa agcggcccag ggctgattgt
      841 cgaggtgaat gtcatgcatg cacaaacgct cccggttgtt cgtatcaatg gtgtggaaat
      901 cagtgagcag gccatcgcgg cggagatgca gtatcatccc gccgccacgc tcgaacaggc
      961 ccgtcaggcc gccagttccg cgttggccgt gcgggaattg ttattgcagg aagccagccg
     1021 gcaagggtgc gacggcgaac cggatgacat gatcgaccag ctgttggcac aggcattgaa
     1081 tctgccgcaa gccggcgagc aggaatgtcg tcaatattat gcgtccaatc cgcagcgctt
     1141 tctcagtccc acactgctcg aagtgcgaca tatcctgctg cccgctgcac ccgatgatgc
     1201 ggacgcgcgc cagcaggcgc tggagaaggc tcgggcgctc atcgccacgc tgcaacggaa
     1261 tattgccacg ttcgacgaac tggcacggga tcattccgtg tgcccgtcac gggagcaggg
     1321 cggttccctc ggtcagctca gcaagggttc cactgtgccg gaattcgaac ggcaggtatt
     1381 tgcggcgcaa cccggcctga tgacgcaacc ggtggagacc cgctacggct atcacgtcgt
     1441 gcaggtggaa cgcaaaattc caggcgaacg cctgccttac gagaatgtcc gcgaccaggt
     1501 gcgccgctat cttgagcaga aggtgtttta cacggcactc agccagtatc tgcgcctgct
     1561 gacggcagcg gccgccattg agggtgtcgc actggaagcc gccacgtcac cactgctgca
     1621 atagcgccgt cttctgctcg ttgttctgcc gcgtgcgcca cgacatttgc gtggcgcgtc
     1681 tttgttgcct acttttactg acgaaggaat ggttcgcgtt gccaaacgcg tagaattagt
     1741 agggaatttc caggatggtg caacccttct cccggcatta cacccgtcac tccgtgctgg
     1801 tcgcgctggt ggcctcgggt gtgtgtggcg ggttggcttt cctgctgctc ctgctgggcc
     1861 tgcacccggc ggtggctgtc tttttcatgg tctccatgct gttgctgctg gccttcgccg
     1921 cctaccttca tgcacgtgtg tttcagtact ccacctatct gcaggacatt ctggaagcgg
     1981 cgccctctgg cattctcgcc atcgatgaac agggttgtgt ccgctttgcc aatcccgctg
     2041 cgcggcagat ttttgaatac gagaaggatg aactggccgg actgtcggtg gaaaagctga
     2101 ttccttccag cattcgcgac aagcatccac aactgcgatc cagtttcgtc agggagcggc
     2161 gcagccgacg catgggcgag gggcgcgaca ttcatggcgt tacccgtagc ggcgctgaag
     2221 ttccgctgga aatcgggttg acctttttgt ccaccggcgc atctcccatg atcatcgtcg
     2281 gcgtgatcga cattcgcgaa ctcaaggcgg cgcagaccac catcgtgcgc cagaacatga
     2341 atctggcgcg ctcggtaaaa gagctcgaac agtttgcatt tatcgcctcg catgatctgc
     2401 gcgagccgct gcgcaaggtg atgaattatg ccgaaatcat gcaggaggat tacggcaata
     2461 tcatcgacga ggatggccgc acggtcctga aatccatgtc gggtgccgtg cagcgtatgg
     2521 aacgactgct cgacagcctg ctgaattact cccgcatcac cacccgggcg cagccgttcg
     2581 aagaggtttc gatgatggat gtgattgccg acgtggtgga ggatctgtct ctcagcatca
     2641 gtgaatccgg cgccaaggtg atttgcagtg ggttgcctgc cgtgatgggt gatcgcaacc
     2701 agttgcggca gctgttccag aaccttatat ccaacagtct taaatatcat cgcgacaatg
     2761 ttgctccggt tatcagaata gaatcgaata accggccggc gcccaatgcg gatccgcact
     2821 atgttgcaat tctggtgcgc gacaacggaa tcggattcga caatcagtac agcgaactta
     2881 ttttcgatgt tttcaagcgg ttgcatgggc gcgacaaata tcagggtacc ggcatggggt
     2941 tggcgatctg ccgcaagatt gtcgaacggc acgagggttt catcgttgca tccggccagg
     3001 tggatgcggg cgccgagttt tgcatctggc tgaaaagggg tagagtcaaa taaatcaagg
     3061 aatcaatgat gccggtttgt gaattttgca cagataacgt gggttcggtt gtgtatggtc
     3121 aaggtttgac caatactggt ttcaaccgcg ctcttagctc atgggccggg acaggatggg
     3181 ttatgacagg catgacagca ccatcggagg cggcagcgct ggctgcgatt ctgattgtgg
     3241 acgacgactt ggaagacgtg cagttcatca ggcgcgcttt caaaaagaac aacgtggcaa
     3301 atccgttgtt ttcagttttt tcaggcgaag aggcgctgga tttcctgggc cggtgcgaca
     3361 gagggagtca ggcttatccc ggtctgattc tgcttgatct caacatgccg ggtgtgggtg
     3421 gaatggaaac cttgcgccgg gtcaagatga acgagcgctg gcgccggatt ccggcagtca
     3481 ttttcactac ctcaaaggcc gaagaggacg tgctcacgtc ctacagcatg ggtgccaact
     3541 cctacatcag caaacctttc ggtttcgacg gtttgaccga ggtggtggcg cagatcagct
     3601 cctactggct gggtgttgca gccctgccat caaaatcagc agagtgagag atgcaaacac
     3661 agttgcgggc acccgcggtg aagaccccgg atcagaatct gaaactgttg ctggtggatg
     3721 acgatgacga ggatctgtat ttcctgaagc gtctgctcaa gcagtcgggc cgcagtcacg
     3781 acgtgctgga agcacgcaat gtggatcagg cggtcgagca gctgcagcat cagccggaca
     3841 ttgtgctgct ggatttttac ctgggtcacc agacggcctt tgacctgctg cagcgcatgc
     3901 agaaatccgg ctgccagtta ccggtaatcc tgctgaccgg ccatgacgat gcggaaatcg
     3961 atcagctggc gctgaataaa ggtatcgccg attatctgcc caagcgcatc gtcaataccg
     4021 ccatactcga ccgttccatt cgttacgccc accgtgattt ccagcgtctg caacaactgg
     4081 aatacctggc ccactacgac acgctcaccg gcctgtgcaa ccggcgcctg ttcatggacc
     4141 ggctgcagca tgcctatgaa aatgcccgac gccagaacag aaccggtgcc ctgttgtata
     4201 tcgacgtgga tggcttcaag cagatcaatg acgaactggg ccacgccacc ggcgacgaac
     4261 tgctgaaagt ggtggcggaa cgcatgagcc tggtagtgcg cagcgccgat acggtcgcac
     4321 gttttggtgg cgatgaattc gtgatcctgc tggaaaacat tggcgcacct gaagcgcacc
     4381 gcgtggcgca aaagctgctt tattcaatgg aaaagccggt gcagctgcgg tcggggccgg
     4441 tgcagtgctc gctgtccatc ggcctgtgcg gattttcggc cgaatacggt tcaccggacg
     4501 aaatactttc cagcgccgac cgcgccctgt atcaggccaa gaccatcggc aagcatacct
     4561 actgttcctt caaccagcgg ttgatggaat ccctcactcg ccagaccctg atcgagcggc
     4621 tggcccagca ggcagtggac agtggcgatt tttttatgca ttaccagccg cgcatgagca
     4681 gccagggcaa tggggtggtg gggttcgaag cgctggcgcg ctggccccgg cgtgacgacg
     4741 ttcagctcac gcccggtgat ttcattcctg ttctggaagc gctgggcctg atggaccgct
     4801 ttacccagtg ggtgatcgac aaggcgctgg cggatctgcc ccggctgcgg cagtgggcgc
     4861 cgggctgcgt gctgtcgctg aatgtatcct caagcagttt cgccagcgac agcgtgaagg
     4921 attacctgct gcagaaactg cagcaactgc aaatcgatcc gtccggcatt gaagtggagg
     4981 tgacggaaag cctcttcatt gagcggcccg aatcggccgt gcgcattctg actgaattgc
     5041 gggcagtagg ggtgcgcgtg gcgctggacg atttcggtac cggctattcc tcgctgagtt
     5101 atctcaacca gctgccgatc gacaccatca agctggaccg gcagtttctg gaacaggtgc
     5161 cggcggagcc ccgcagtacc ctgctggtgg ccgcgatcgt cgatctggcc ggcaacctgc
     5221 aactgaacgt ggtagcggaa ggggtggagc gcgaaccaca gaaagcattt ctgcaatcct
     5281 gcggttgcca tgaaatgcag ggatacctga ccggccggcc catgtcagtg gaccagctgc
     5341 aggcgttctt ttccaaaccg gattaaccgt ctctcagtcc gttcacgacg cggaactcgt
     5401 ctgccagttc ctcgggggac tggatgctga cgcccagatc cttggccagt ccgtcccgca
     5461 ggctgtaaat ccagccgtgg atgttcaggg gctggccccg ttcccaggcc cgctgcacca
     5521 catgggtgtg cgccacgttc ttcacctgct gcatgacatt caattcacac aggaaattca
     5581 ggcggtcttc ctcgcggtcg atggaatcca tgcggtactt gttgcggtag tacaggttct
     5641 tgatgttgta gagccagttg tcgatcaggc cgtgatgggt gccgtccagc gcggcacgga
     5701 taccgccgca gccataatgc cccaccacca gtacgtgttt caccttcagc acttccaccg
     5761 catattccag caccgacatg cagttgaaat ccgtgtgcac caccaggttg gcgatattgc
     5821 ggtgcacgaa cacctcgccg ggcagcaggc cgatgatctc gttggcgggc acgcggctgt
     5881 ctgcgcagcc gatccacagg tattccgggg cctgggtttt cgacaggcgt tcaaagaagt
     5941 gcggatcgtc tgctttcacc cggtctgccc aggcaacatt gtttgcaagc agttcgtaaa
     6001 gcttggccat aaaaggactc ccaccgattg catttcaggt cgccgaaatc atagtgaccg
     6061 acaggggggg ctgtcgatca ctacaatgcg cggaaatgta tctccttttt acaaagacga
     6121 gggtgcaaac accgcgcggt agacgtcctg gtagtggtcg gcaaaatgca cggtgatgcc
     6181 cttgcggatg taatcgggca attcatcgta atcgcgccgg ttggcttcgg gcagaatcag
     6241 ctcgcggatt ttctggcgcc gggccgcaat caccttctcg cggatacctc caacaggcag
     6301 cacctgcccc gtcagggtaa tctcgcccgt cattgccagt gggcggggca ggctccggtt
     6361 gcgggccagc gaaatcagtg cggtggtcat ggtgacaccg gcgctggggc cgtccttggg
     6421 cgtggcaccc tcgggcacgt ggacgtggat ggctgctttg tcgaagaaag cgggatcacc
     6481 gccatactgc tggatgtgcg acgcggcgta actgtaggcg atctcggcgg attcc
//
LOCUS       DASUEG010000536         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_516652, whole genome shotgun sequence.
ACCESSION   DASUEG010000536 DASUEG010000000
VERSION     DASUEG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516652"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="VFX11_10100"
     CDS             complement(<1..1730)
                     /locus_tag="VFX11_10100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939018.1"
                     /translation="MKQNILIVDDQRPNLVALEATLAELDINIIAATSGPEALSILLK
                     QNVALVLLDVQMPGMDGFEVAGLMRQRGKTRHTPIIFLTAINKEDSYLFKGYESGGVD
                     FIFKPYDPFVLISKVRIFLELDLRRQQLEEALNKLKQLKTSNEILLRSVGEGIIGVAA
                     NGVISFVNPAAEIVLGCVATDLIGQPVYKSVIVSAQQVDQKGWEDSKIYKFCSQGQAY
                     HTDVAVFRTFANRMLPVEYTASPTHLPNGGFDGVVIVFKDITERKKIEEKLNYMAQYD
                     ALTGLGNRNLFGSALNNAITFSKHSGQSFALLFMDLDRFKQVNDTLGHHAGDQLLKEV
                     ALRVSDCIRDTDILCRLGGDEFTIIINGKAAEPASLRVSEKLIGALSRPFEIFGQEIY
                     VGASIGIVYFPGMGEDAHELIKNADMAMYQAKHEGRNRFRVFEQGMRAQIEESMALEL
                     ELRRATDTMDFFLHYQPKIDMHSGDIVGLEALIRWRIGERMISPAVFIPVAEETGLIG
                     SIGRWVFETACTQVRAWHDAFDLPEGFRVSVNMSVQQLQDPNLVHELTRIMEHCQVQG
                     DWMELEITESL"
     gene            complement(1727..2308)
                     /locus_tag="VFX11_10105"
     CDS             complement(1727..2308)
                     /locus_tag="VFX11_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019409689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX4939019.1"
                     /translation="MWNQQLVVVGASSGGLDALLTLLAALSPDYCLPLLVVLHQRANR
                     ESGVPAMLARHTHLQVLEVEDKLSISSGHLYVAPPNYHVLIEKEKLLSLSIEPPVNYC
                     RPSIDVTLDSAAFVYRERLVACVLSGANQDGADGAREVKRRGGRVFVQDTAEAQVSTM
                     PQAVAAAVSVDGMLSMGGMAAMLNRMNRSECAT"
     gene            complement(2326..>2568)
                     /locus_tag="VFX11_10110"
     CDS             complement(2326..>2568)
                     /locus_tag="VFX11_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4939020.1"
                     /translation="FTTHDLVTDQVFGEMQLVICRNVLIYFRRELQSRVLTLFYESLD
                     MGGFLGLGIKESLSALPNQNSFRIVDSAVRLFQRID"
BASE COUNT          553 a          783 c          684 g          548 t
ORIGIN      
        1 aggctttcgg ttatttccag ttccatccag tcgccctgca cctggcaatg ctccatgatg
       61 cgggtcagct catgcaccag attcgggtcc tgcagctgct gcaccgacat attgacggag
      121 acacggaatc cttccggcag atcgaatgcg tcatgccagg cacgcacctg tgtgcaggcg
      181 gtttcgaaca cccagcgccc gatgctgccg atcaggcctg tctcctccgc cacgggaatg
      241 aagacggcgg gtgaaatcat ccgttcgccg atgcgccagc gaatcaacgc ttcaagcccg
      301 acgatgtcgc cgctgtgcat gtcgatcttg ggctggtagt gcaggaaaaa atccatggtg
      361 tcggttgcac ggcgcaattc cagctccagc gccatggatt cctcgatctg cgccctcatg
      421 ccctgctcga acacccggaa acggttgcgc ccttcgtgct tggcctggta catggccatg
      481 tcggcgttct tgatcagctc atgggcgtcc tcacccattc ccgggaaata gacaattcca
      541 atactggcac ccacgtaaat ttcctgacca aaaatttcga atggacgtga cagtgcgcca
      601 atcagttttt cggaaacccg cagcgacgcc ggttccgccg ccttgccgtt gatgatgatg
      661 gtgaattcgt cgccgcccag acggcacagt atgtcggtgt cccggatgca atcgcttacg
      721 cgcagcgcca cctccttcag caactggtcg cccgcatgat gacccagcgt gtcattgacc
      781 tgcttgaaac gatccagatc catgaacagc agggcaaagg actggccact gtgcttgctg
      841 aaggtaatcg cgttgttcag cgccgagccg aacagattgc ggttgccaag cccggtcagc
      901 gcgtcgtact gcgccatgta attgagcttt tcctcgatct tcttgcgctc ggtgatgtcc
      961 ttgaacacga tgaccacgcc atcgaaaccg ccattgggca ggtgcgtggg gctcgcagtg
     1021 tattccaccg gcaacatgcg gttcgcaaag gtgcggaaca cagccacatc ggtgtgatag
     1081 gcctggccct gggaacagaa cttgtagatc ttcgaatcct cccagccttt ctggtccact
     1141 tgctgtgcgg acacgatgac cgatttgtaa accggttgcc cgatcagatc ggtcgcgaca
     1201 cagcccagta caatttcagc ggcgggattg acgaaactga taacgccgtt ggcggcgaca
     1261 ccgatgatgc cttcacccac ggaccgcagc aggatttcat tgctggtttt gagctgcttg
     1321 agcttgttga gtgcttcttc cagctgctgc cggcgcagat ccagctcaag aaaaatgcgc
     1381 accttgctga tgagaacaaa gggatcatag ggtttgaata tgaaatcgac gccgccgctc
     1441 tcataaccct tgaacaggta gctgtcttcc ttgttgatgg cagtgaggaa aatgatgggc
     1501 gtgtgtcggg tcttgccgcg ctgccgcatc agacctgcca cttcgaaacc atccatgcca
     1561 ggcatctgca catccagcag cacaagcgcc acattctgct tgagcagaat cgacagggct
     1621 tcagggcctg acgtggcggc aatgatattg atatccagct ccgccagcgt cgcttccagc
     1681 gccaccagat tggggcgttg atcgtccacg atcagaatat tctgcttcat gttgcgcact
     1741 ccgaccggtt catgcggttc agcattgctg ccatgccgcc catcgacaac atgccatcga
     1801 cactgaccgc cgccgctact gcctggggca tcgtcgacac ctgcgcctct gcggtatcct
     1861 gcacaaacac gcggccaccg cgacgcttga cttcgcgcgc gccgtccgcg ccgtcctggt
     1921 ttgcgccgct gagcacgcac gcgacaagcc gctcacgata gacgaacgcg gcggaatcca
     1981 gcgtgacgtc aatggagggt cggcagtaat tcacaggcgg ctcgatactc aaggacaata
     2041 acttctcttt ttctatcaac acatgataat tcggtggcgc cacatacagg tgacccgacg
     2101 aaatcgacaa cttgtcttcc acctccagaa cctgcaaatg ggtatgtcgc gccagcatgg
     2161 cgggcactcc actttcccgg ttggctctct ggtgcaacac caccagcagg ggcaggcaat
     2221 aatcgggtga caacgctgcc agcagcgtca gcaaggcgtc cagtccgcca ctcgatgcac
     2281 ccacgacgac aagctgctga ttccacatgt ccctgccgcc attcctcagt cgatgcgctg
     2341 aaacagccgc actgctgaat caacgattcg gaaactattt tggtttggca gggcgctcag
     2401 ggattccttg atccccagcc cgagaaatcc acccatatcc agactttcat agaataaggt
     2461 tagcacccgg gattgaagtt cgcggcgaaa gtagatcagc acatttcgac agatcaccag
     2521 ctgcatctcg ccgaagacct gatctgtcac caggtcatgg gtggtgaa
//
LOCUS       DASUEG010000537         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_851541, whole genome shotgun sequence.
ACCESSION   DASUEG010000537 DASUEG010000000
VERSION     DASUEG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851541"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFX11_10115"
     CDS             complement(<1..244)
                     /locus_tag="VFX11_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939021.1"
                     /translation="MLPTRLGIVSLLLVVPLAAMLIQLMTVRNDQLVQVERKLRTVQD
                     IRTLQAVLDNAERLRDISIVAVYDRNDELAGEYAIQR"
     gene            complement(448..927)
                     /locus_tag="VFX11_10120"
     CDS             complement(448..927)
                     /locus_tag="VFX11_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4939022.1"
                     /translation="MVNMKQLVASAVMVVGVALSGQALASKLADQTGNAEPMGTRSIM
                     SPTKIDARTQPSAKVCVQGEECGGAAAPVAAAAAAPKSPEEIYNGSCMACHATGAAGA
                     PKVGDAAAWAPRIAQGADTLHKHAIGGFNMMPPKGTCAACTDDEIMAVVDFMVSKSK"
     gene            complement(1037..1597)
                     /locus_tag="VFX11_10125"
     CDS             complement(1037..1597)
                     /locus_tag="VFX11_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX4939023.1"
                     /translation="MRSRTLPRIVILLLLLALALWLGGCAPYQTRIDFDPAARLTELK
                     TYAWVPADQSVAAGESYQSLDQVRLRRAFEEALAAKGLQPAPVAQAQVWVDLNYEINR
                     RYEAQTVYYGYYGWHPYWWGMEPDTRIDERDESRLSLIMINPQTRSVVWTGQATVRYY
                     EDHPPKERNTRLVEQVQAVMEQFPPR"
     gene            complement(1594..2142)
                     /locus_tag="VFX11_10130"
     CDS             complement(1594..2142)
                     /locus_tag="VFX11_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX4939024.1"
                     /translation="MPRLLLPLLSLLLLFLTACAQDPVSFDYDQGAYLKTLKTYAISA
                     PTSDGGFQSLDNSRIEAALRTALSGRQMQEVPADKAEFLVSYRVEPDRQLDRSGVSFG
                     FGVSSGGGGMGGIGVGASTGPKAREVVEGKLVVDVIDPRKKQVVWSARANENLTDSMS
                     SAKRDELIGFLVNAMFERFPPQ"
     gene            2333..2409
                     /locus_tag="VFX11_10135"
     tRNA            2333..2409
                     /locus_tag="VFX11_10135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2367..2369,aa:Arg,seq:ccg)
     gene            2648..3307
                     /locus_tag="VFX11_10140"
     CDS             2648..3307
                     /locus_tag="VFX11_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4939025.1"
                     /translation="MADPIPAIATPGPLTERMGMSLAQAFHDAPDYRFILACHPDRLS
                     ALSWFFGSFVARLALRYGSVLSTDIGGAGILLFAPGQSPSLPALLGAGALSLPRFFGL
                     RGAWRAFFLGLHLERRRLQLAPLPHWYVIAVGVTPELQGRGNGIALLKRALQRSDADG
                     VPCYLEVFEAELVTRYERMGFVVLHQDRLPGELTLWCMLRPPGALANTAATFSNGSFT
                     L"
     gene            3324..>3918
                     /locus_tag="VFX11_10145"
     CDS             3324..>3918
                     /locus_tag="VFX11_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939026.1"
                     /translation="MTEYNSIFHALQTRPATHPTQPVFSYANENGQIEKTLNWQEFAG
                     RIDALTGFFRTPLDLQAGDRVLLVYPPSLDFVLAFAACLRAGLVPVPVYPPNPRKLDS
                     GTEAFLRIAQDCDARLVLTSTQYEQNRNRGGLQELAATDPARWSLALRWVTTDQINAG
                     DFVAVHGAEPAPNDVALIQYTSGSTSAPKGVVITHGNL"
BASE COUNT          708 a         1269 c         1170 g          771 t
ORIGIN      
        1 gacgctggat ggcatattcc cccgccagtt cgtcgttacg gtcatatacg gcaacgatgc
       61 tgatgtcgcg cagtcgctcg gcgttatcca gcaccgcctg cagggtgcgg atgtcctgca
      121 ccgtgcgcag cttgcgctcc acctgcacca gctggtcgtt gcgcaccgtc atcaactgga
      181 tcagcatggc cgccagcggc accaccagca gcagcgagac aatccccagc cgggtgggca
      241 acatcagccg ccccctctgc cgggtcccgc ttgccctgat cagtgccgac atcgtgcctg
      301 ccttgacatt ggtattgttg ttgagtccgg cccagggatc tggcgcccgt ctttaccagt
      361 atagccaacc tgaacccggt gatttaaaac gaaaaagccc cgcgggttcg ccgcagggct
      421 tctttatgac ggagggcaat gccccgctta cttggatttg ctcaccatga agtccaccac
      481 cgccatgatc tcgtcatccg tgcaggcagc gcaggtacct ttgggtggca tcatgttgaa
      541 tccgccgata gcgtgtttgt gcagggtgtc ggcaccctgg gcaatgcgcg gtgcccaggc
      601 agcggcgtcg cccaccttgg gagcgccggc agcgccagtg gcgtggcagg ccatgcagga
      661 accgttatag atttcctccg gtgacttggg agcagccgca gccgcggcca ccggggcggc
      721 ggccccgccg cactcctcac cctgcacgca caccttcgcc gacggctggg tacgggcatc
      781 gatcttggtc gggctcatga tggagcgggt tcccatcggt tcggcattgc cggtctgatc
      841 cgccagcttc gacgccagcg cctgacccga cagggccaca cccaccacca ttactgccga
      901 tgccaccagt tgcttcatgt ttaccatgtt gtaaaaccct ctctccggaa tgcttcgtac
      961 ctggaagagc cccgaccgaa gcggttagca aaattggacg ggcgcaagta taccgtcagt
     1021 tacctgaaaa acggaatcag cgcggcggaa actgctccat cactgcctgt acctgctcca
     1081 ccagacgggt attgcgctct ttggggggat gatcttcgta atagcggacc gtggcctggc
     1141 cagtccagac caccgaccgg gtctggggat tgatcatgat cagggacagc cggctctcgt
     1201 cgcgctcgtc gatgcgggta tcgggctcca tgccccacca gtagggatgc cagccgtagt
     1261 agccgtaata gacggtctgc gcctcatagc gccggttgat ctcgtaattg aggtccaccc
     1321 agacctgcgc ctgcgccacc ggcgccggct gcaggccctt ggccgccagc gcctcctcga
     1381 aggcccggcg cagccgcacc tggtccagcg actggtaact ctcgcccgcc gccaccgatt
     1441 gatccgctgg cacccaggcg taggttttca gttctgtcag ccgggccgcc ggatcgaaat
     1501 cgatccgggt ctggtagggc gcgcagcccc ccagccacag ggccagcgcc agcaggagca
     1561 gcaaaatgac aatgcggggc aacgtgcggg atctcattgg ggcgggaacc gttcgaacat
     1621 ggcattgacc agaaagccga tcagctcatc gcgcttggcg gagctcatgg aatcggtgag
     1681 gttttcgttg gcgcgggcgc tccagaccac ctgctttttc cggggatcga ttacatccac
     1741 caccagtttg ccttccacca cttcccgggc cttgggcccg gtgctggcac ccacgccgat
     1801 gccgcccatg ccgcccccgc cactggacac gccgaagcca aaactgacac cggaacgatc
     1861 cagctggcga tccggctcca cgcggtagga caccaggaat tccgccttgt ctgcgggcac
     1921 ttcctgcatc tggcggcccg aaagtgccgt gcgcagggcg gcttcaatgc ggctgttgtc
     1981 gagggactgg aagccgccat ccgacgtcgg ggccgagatg gcataggttt tcagggtttt
     2041 gaggtaagcg ccctgatcgt aatcgaagga caccgggtcc tgggcgcagg cggtcaggaa
     2101 caatagaagg agggagagca gaggcagtag aaggcgcggc atggcaactc cttgcgcggc
     2161 ggtggaaaga actacttgag aggcaacaga ttagcgaaat ccgcctgcgg aggaaaccgc
     2221 gcactgcatg gactttgccg gatcaagcgc ttattatagc gcccctcgac ttccgccggc
     2281 ttgccgtggc ggggcgattc ctgcaatacc cccttgtttg accgcacgta gtgcgcccgt
     2341 agctcagctg gatagagcgt cgccctccgg aggcaaaggt cagaggttcg aatcctctcg
     2401 ggcgcgccat ataagccagg cgcctagaaa gtttcttcct gccgatccag ccttttacag
     2461 ccgaacaggg tacacatcag cgttgaaaac cgttttgttg gccgaccgcg gaaatgattc
     2521 tgcattttca ggtgcgctcg agtaacacac cctcttccct ccgtcaatcc aacttaagtg
     2581 ttcaagtttt ccacaccccc tcacctagac tgcgcgcaat ctcgatttcg cacgcaggct
     2641 ttcaaccatg gcagacccga ttccggcaat tgctacgcca ggccccctca ccgagcgcat
     2701 gggcatgtcc ctggcgcagg cctttcacga cgcgccggat taccgcttca tcctggcctg
     2761 ccatcctgac cgcctgtcgg cactgtcctg gtttttcggt tcattcgtgg cgcggctggc
     2821 gctgcgttac ggcagcgtgc tgagcaccga catcggcggc gccggcattt tgctgtttgc
     2881 accggggcaa tccccttcac tgcccgcact gctgggcgcc ggcgcgctgt cgctgccgcg
     2941 tttttttgga ctgcgcggtg cctggcgcgc cttttttctt ggactgcatc tggaacggcg
     3001 gcgcctgcaa ctggcaccgc tgccgcactg gtatgtgatc gccgttggcg tgacgccgga
     3061 actgcaaggg cgcggcaatg gcatcgctct gctgaaacgc gcgctgcaac ggtccgacgc
     3121 agatggcgtg ccctgttacc tggaagtgtt tgaagcggaa ctggtgacgc gctacgagcg
     3181 catgggattt gtcgtattgc atcaggaccg cctgcccggc gaattgacgc tctggtgcat
     3241 gttgcgcccg cccggtgcgc tcgccaacac cgccgccacg ttttcgaacg gatccttcac
     3301 tctttgaaaa ggaagtcacc gccatgacgg aatacaacag catttttcac gccctgcaaa
     3361 cccgccccgc cacgcacccg acccagccgg tgttttccta cgccaatgaa aacgggcaga
     3421 ttgaaaagac attgaactgg caagaattcg ccggccgcat cgatgcactg acgggtttct
     3481 tccgcacgcc actggatctg caggccggtg atcgcgtgct gctggtgtat ccgccttcgc
     3541 tggatttcgt gctggcgttt gccgcctgcc tgcgcgcagg tctggtgccg gtgcccgtgt
     3601 atccgcccaa tccgcgcaag ctggacagcg gcacggaagc gttcctgcgc atcgcccagg
     3661 attgcgacgc gcgtctggtg ctgaccagta cgcagtacga acaaaatcgc aatcgcggcg
     3721 gactgcagga actggccgcc actgatccgg cacgctggtc gctggcactg cgctgggtga
     3781 cgacggacca gatcaatgcc ggtgattttg ttgcggtgca cggcgcagaa cccgcgccga
     3841 atgacgtggc gctgattcag tacacctccg gttccacctc tgcgcccaag ggcgtggtga
     3901 tcacgcacgg caatctgt
//
LOCUS       DASUEG010000538         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_665048, whole genome shotgun sequence.
ACCESSION   DASUEG010000538 DASUEG010000000
VERSION     DASUEG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665048"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFX11_10150"
     CDS             <1..636
                     /locus_tag="VFX11_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX4939027.1"
                     /translation="QARKCNLWILGGTIPKRTLADGKPVPGWRVRAASILVNPQGEMV
                     ARYDKMHLFDVQVSDAQGQYRESATIEPGDVPVLATTPFGKLGMTVCYDLRFPELFSL
                     LQRQGADFFTVPSAFTWRTGEAHWEILLRARAIETQSYIIAPGQGGVHSSKRTTWGHS
                     MIIDPWGMVLGDKTEAGPGVVLAEMDSELLQSVRNSMPIAQHKRFQVVSKP"
     gene            633..2078
                     /gene="tldD"
                     /locus_tag="VFX11_10155"
     CDS             633..2078
                     /gene="tldD"
                     /locus_tag="VFX11_10155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX4939028.1"
                     /translation="MISRIDDRLAIAQQTLWQPSALDTPRLTRVLEQMLARGADYADL
                     YFQRGVSESWSLEESEVKDGSYSVSGGVGVRALSQEKCGFSYSDQIELNAIEQASQAA
                     ASVAQGQGGVIQTIAPRQTPALYVGTNPLDTLSRDQKVQLLQQVDQLARQADPRVIDV
                     SVTLAASFQTILVLASDGTLAADVRPLVRLSVSVILQHNGRRERGSCGGGGRCGYELF
                     DADTLSFYAREAVRMAQVNLEAEAAPAGTMPVVVGAGWPGVLLHEAVGHGLEGDFNRK
                     GTSNYSGRIGTPVASPLCTIVDDGTLPNLRGSLSVDDEGTPTHCTTLVEKGVLRAYLQ
                     DKLNARLMGHPLTGNGRRESYAHLPMPRMTNTYLLAGQDDPADIIRSVKKGLYAVNFG
                     GGQVDITSGKFTFAASEAYLIEDGKITRPVKGATLIGNGPEALSKVSMLGHDLALDRG
                     VGTCGKEGQSVPVGVGQPTLKVDELTVGGTR"
     gene            complement(2090..2629)
                     /gene="yjgA"
                     /locus_tag="VFX11_10160"
     CDS             complement(2090..2629)
                     /gene="yjgA"
                     /locus_tag="VFX11_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX4939029.1"
                     /translation="MIDYDKPNDDQDQDLPPSKGQLKRETEHLQKVGEKLLYLSDAQL
                     AKLPLTDKLIAAVEEGKRIKSNSEALRRHKQYIGKIIREADTATIEARIKEFENAHSL
                     NTRQFHDLENLRDALITGDNNVIGEVIAKFPDVDSPKLRQLVRNAKKERDQNLAHPEK
                     PENTHGRKLFRYLRELSNL"
     gene            2967..>3581
                     /locus_tag="VFX11_10165"
     CDS             2967..>3581
                     /locus_tag="VFX11_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939030.1"
                     /translation="MESRRDMAGIWPGRTWAGAVVSLFLGLWLTACGGGGGGGGHSEN
                     RGTTEPMVVIDGQVALEGQSMEGAVITLSRIDVSRTPISENVKGQQVVVDLPEGKPTV
                     GPQGDLQFQVPASALVDDQLYSVTVACQPVRGRCSMQMPVHVVLSGARLQQGGWKLNV
                     PTEVAYQRLAYYVAARFNESDLKQEMDTTAKLMLKADVDGSGGID"
BASE COUNT          673 a          966 c         1206 g          736 t
ORIGIN      
        1 caggcgcgga aatgtaacct ctggattctg ggcggcacca ttcccaagcg cacgctggcg
       61 gacggcaagc ctgtgcctgg ctggcgcgtg cgtgcagcat ccattctggt gaatccgcag
      121 ggtgaaatgg tggcgcgcta cgacaagatg cacctgttcg atgtgcaggt cagcgatgcg
      181 cagggccaat accgcgaatc tgccaccatt gagccgggcg atgtgccggt gctggcgacc
      241 acgccattcg gcaagctcgg catgaccgtc tgctacgatc tgcgttttcc cgaactgttc
      301 agcctgctgc agcggcaggg cgcggatttt ttcacggtgc cgtcggcgtt cacctggcgc
      361 accggcgagg cccactggga aattctgctg cgggcccgcg cgattgaaac ccaaagctac
      421 atcatcgcgc cggggcaggg tggcgtgcac tcatccaagc ggacgacatg gggccattcc
      481 atgatcatcg atccctgggg catggtgctg ggggacaaaa cggaagccgg ccccggcgtt
      541 gtgctggcgg aaatggacag cgaattgctg cagagcgtgc gcaacagcat gccgatcgcg
      601 cagcacaaac gatttcaggt ggtgagcaaa ccatgatttc ccgcatcgac gatcgtctgg
      661 ccattgcgca acaaaccctg tggcaaccca gcgcgctgga caccccgcga ctcacacgcg
      721 tgctggaaca gatgctggcg cgcggcgcgg attatgccga tctgtatttt cagcgcggcg
      781 tcagcgaatc ctggagcctg gaagaaagtg aagtgaagga tggcagctac agcgtcagtg
      841 gcggcgttgg cgtgcgtgca ctcagtcagg aaaagtgcgg cttttcctat tccgatcaga
      901 ttgaactgaa tgccatcgag caggcgtcgc aggcggcagc atcggtggcg cagggacagg
      961 gcggtgtgat tcaaaccatt gcaccgcgcc agacacccgc gctgtatgtt ggcaccaatc
     1021 cgctcgacac gctttcccgc gaccagaaag tgcaattgct gcagcaggtg gatcagctgg
     1081 cacggcaggc cgatccgcgt gtgatcgatg tcagcgtcac gctggcggct tccttccaga
     1141 ccattctggt actggccagt gacggcacgc tggcggcgga cgtgcgtccg ctggtgcgtc
     1201 tgagtgtgtc ggtgatcctg cagcacaacg gccggcgcga gcgcggcagc tgcggcggcg
     1261 gtggtcgttg cggttatgaa ctgtttgatg cggacacgtt gagtttttac gcacgcgaag
     1321 ccgtgcgcat ggcgcaggtg aatctggaag ccgaagcggc gccggccggg accatgccgg
     1381 tagtggtggg cgcgggctgg cccggcgtgc tgctgcacga agcggtgggg cacgggctgg
     1441 aaggcgattt caaccgcaag ggcacctcga attattccgg ccgcataggc acgccggtgg
     1501 cttcgccact gtgcaccatc gtggatgacg gcacgctgcc gaatctgcgc ggctctttat
     1561 ccgtcgatga tgaaggcacg ccgacccatt gcaccacgct ggtggaaaaa ggcgtgctgc
     1621 gcgcctatct gcaggacaag ctcaatgccc ggttgatggg gcatcccctc accggcaatg
     1681 gccgacgcga atcctacgcg catctgccca tgccacgtat gaccaacacc tacctgctgg
     1741 ccgggcagga cgatccggcc gacatcatcc ggtcagtgaa aaaagggttg tatgcggtga
     1801 atttcggcgg tggtcaggtg gatatcacct cgggcaaatt caccttcgcc gccagcgaag
     1861 cctatctgat cgaggacggc aaaatcaccc gcccggtgaa aggcgcgaca ctgatcggca
     1921 atggcccgga ggcgctgtcg aaagtgtcga tgctgggcca tgatctggcg ctggaccgtg
     1981 gcgttggcac gtgcggcaag gaaggccagt cagtgccggt gggcgtgggc cagccgacac
     2041 tgaaagtgga tgaattgacg gtgggtggaa cgcgctgaac cggcgcgtat cagagattgc
     2101 tgagttcccg cagatagcgg aacagcttgc ggccgtgggt gttctcgggt ttttccgggt
     2161 gagccagatt ctggtcccgt tccttcttcg cgttgcgcac cagttgacgc agcttgggtg
     2221 aatccacatc gggaaatttc gcgatcacct cgccaatgac attgttgtcg cctgtgatca
     2281 acgcatcgcg cagattttcc agatcatgaa actgccgcgt gttcagtgaa tgcgcattct
     2341 cgaattcctt gatccgcgct tcgatggtgg ccgtgtccgc ctcgcggatg atcttgccga
     2401 tgtactgctt gtgacggcgc agcgcctccg agttgctctt gatgcgcttg ccttcctcca
     2461 ccgcggctat cagcttgtcg gtcagcggca gttttgccag ctgcgcgtcg gacagataca
     2521 acagcttctc gcccaccttc tgcaggtgct cggtttcgcg tttgagctgg cctttgctgg
     2581 gggggaggtc ctggtcttga tcgtcgtttg gcttgtcgta atcaatcatg aaggatcgtt
     2641 gtggctggtt aggctgggga aatggcctgc catgatacca cagggggagg ggtttggccg
     2701 ccgcaatggc cctgcaggga aaccgctacc cctgctgacc ggcataggct gtttcccgct
     2761 tgtctgtgcc cgggaaggcc tttattctcg tggcgctgcg cgcggagtct ggctccgtag
     2821 tggcattgcc gttccgatac gaaatggaac aggcggacga gcagcggggg ctgctgggta
     2881 cagtggggac aacggtctgg tatggggcgg tgaccgcagc cgggcgttgc tgatgaattc
     2941 gttgatagac cttatcagga gtggcaatgg aaagcagacg cgacatggct gggatctggc
     3001 cgggacggac atgggctggt gcggtggttt cgctgtttct ggggctgtgg ctgaccgctt
     3061 gtggtggtgg gggtggtggt ggcggtcaca gcgaaaaccg gggtactact gaacccatgg
     3121 tggtgattga cggacaggtg gcattggaag gtcaatcgat ggagggagcc gtgattaccc
     3181 tgagccgtat cgacgtgtcc cgcacgccca tttctgagaa tgtaaaaggt cagcaggttg
     3241 tagtggatct accagagggt aaaccgacgg tgggcccgca gggcgatctt caattccagg
     3301 tgcctgcaag tgcgttggta gatgaccagc tatacagcgt gacagtggcc tgccagccgg
     3361 tgagagggcg ttgctctatg caaatgccag tgcatgttgt gctgagtgga gcacggctgc
     3421 agcagggtgg atggaaattg aatgttccaa cggaagttgc ttaccagcgg ctggcctact
     3481 acgtggcggc acgtttcaat gagtctgatt tgaagcagga aatggatacg acagccaagc
     3541 tgatgctgaa agcggatgtg gatggttcgg gtgggattga t
//
LOCUS       DASUEG010000539         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_863835, whole genome shotgun sequence.
ACCESSION   DASUEG010000539 DASUEG010000000
VERSION     DASUEG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863835"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2075)
                     /locus_tag="VFX11_10170"
     CDS             complement(<1..>2075)
                     /locus_tag="VFX11_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX4939031.1"
                     /translation="AQQVKNAVIVPVGAKGGFFPKCLPKSGNRDQVMAEVVASYQTFV
                     RGLLDITDNLVDGKVQPPRDVVRKDDDDPYLVVAADKGTASFSDIANALSAEYKFWLG
                     DAFASGGSVGYDHKKMGITAKGGWVSVQRHFRELGIDVQTQSITVLGIGDMAGDVFGN
                     GLLRSRVLKLVAAFNHQHIFIDPNPDPEKSFVERERLFNLPRSSWGDYNQELLSAGGG
                     IFLRSAKSIRVTPQMKERFDIRVDALTPTELISALLKAPVDLIWNGGIGTYVKSSREQ
                     HSDVGDKANDAVRVNGCEMRAKVIGEGGNLGMTQLGRIEYALHGGLSNTDFIDNSGGV
                     DCSDHEVNIKILLNDIVQNGDLTLKQRNQLLVEMTDEVAQLVLENNYRQTQAISIARS
                     ESVYSMGEYRRYIHALVASGKLDRELEFIPNEEELHDRITNGKGLVRPELAILLSYTK
                     AILKDALRDSSLPDEEYVSREIEKAFPQVLTRRFKPQMLQHKLRREIIATQVASGIVD
                     YMGITFVHRMKDAAGSTVADIARAFITARDIFELELWWKQIEDLDHRVDASEQLEMMR
                     MLIRLMRRATRWFLRNNRCGVNVQESIRRFQGGVKTVAESLPTVLRGPRRELWEARHN
                     RYVEKGVPSALATFIAGADSMLPVLGIIQAAEVTGKPVQEVANAYFAIGAQLDLHWFS
                     EEINGLNIE"
BASE COUNT          398 a          662 c          569 g          446 t
ORIGIN      
        1 tttcgatatt gagcccgttg atttcctcgc tgaaccagtg caggtccagc tgcgcgccaa
       61 tggcgaaata ggcgttcgcc acctcctgca ccggcttgcc ggtaacctcc gccgcctgga
      121 tgatgcccag caccggcagc atgctgtcgg cacctgcgat gaaggtggcc agggccgaag
      181 gcacgccttt ttcaacatag cggttgtgac gtgcctccca cagttcgcga cgcggcccgc
      241 gcaatacggt aggcaggctt tctgcaacgg ttttcacccc gccctgaaag cggcggatgg
      301 attcctgcac gttgacgccg cagcggttat tgcgcaggaa ccagcgggtg gcgcggcgca
      361 tcagccggat cagcatccgc atcatttcca gctgctcgct ggcatccacc cgatgatcca
      421 gatcctcgat ctgcttccac cacagttcca gctcgaagat atcccgcgcg gtaatgaagg
      481 cgcgggcgat atccgccacc gtggagcccg cggcatcctt catgcggtgc acgaacgtaa
      541 tgcccatgta atcgacgata ccgctggcca cctgcgtggc aatgatttcg cgccgcagct
      601 tgtgctgcag catctgcggc ttgaagcgcc gggtgagcac ctgcggaaac gccttttcaa
      661 tctcgcgact gacatattcc tcatcgggaa gactggaatc gcgcagggca tccttcagga
      721 tcgccttggt gtatgacagc aggatcgcca gttctggccg caccagcccc ttgccattgg
      781 tgatgcggtc atgcagttct tcctcgttgg ggatgaattc cagctcgcga tccagtttgc
      841 cggatgccac cagcgcatga atgtagcgcc ggtattcgcc catgctgtac acggattcgc
      901 tgcgggcaat actgatggcc tgggtctggc ggtaattgtt ttccagcacc agctgcgcca
      961 cttcgtcggt catttccacc agcagctggt tgcgctgctt cagtgtgaga tcgccattct
     1021 gcacgatgtc gttgagcagg atcttgatat tcacttcatg gtcggaacag tccacaccgc
     1081 cggaattgtc gatgaaatcc gtattcgaca aaccaccatg cagtgcatat tcgatgcgac
     1141 ccagctgtgt catgcccaga ttgccacctt cgccgatcac cttcgcccgc atttcacagc
     1201 cgttcacgcg caccgcatcg ttggccttgt cacccacatc actgtgctgc tcccggctgg
     1261 atttgacgta ggtgccaatg cccccgttcc agatcaggtc caccggtgcc ttcaacagtg
     1321 cgctgatcag ttcggtgggt gtgagcgcat ccacgcgaat atcgaagcgc tccttcatct
     1381 gcggcgtgac ccggatcgat ttggcagacc gcaggaaaat accgccgccg gcggacaaca
     1441 gttcctggtt gtaatcgccc cagctggaac gcggcaggtt gaacaggcgc tcgcgctcga
     1501 caaagctctt ttcaggatcg ggattgggat cgatgaaaat atgctggtga ttaaatgcag
     1561 ccaccagttt cagtacgcgc gaccgcagca ggccgttgcc gaacacgtcg cccgccatgt
     1621 cgccgatacc cagcacggta atggactggg tctgcacatc aatgcccagt tcgcgaaagt
     1681 gccgctgcac tgacacccag ccgccttttg cggtgatgcc catcttcttg tggtcataac
     1741 ccaccgatcc gccggacgca aacgcgtcgc ccagccagaa tttgtattct gccgacaggg
     1801 cgttggcaat gtcggaaaag ctcgcggtgc ccttgtccgc cgccaccacc agatagggat
     1861 catcgtcatc cttgcgcacc acatcgcgcg gaggctgcac cttgccgtcc accaggttgt
     1921 cggtgatgtc cagcaggcca cgcacgaacg tctggtatga cgctaccact tccgccatca
     1981 cctgatcacg attgccgctc ttgggcaggc acttgggaaa gaaaccaccc ttggcgccca
     2041 ccggcacgat cactgcgttt tttacctgtt gcgcc
//
LOCUS       DASUEG010000540         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_140634, whole genome shotgun sequence.
ACCESSION   DASUEG010000540 DASUEG010000000
VERSION     DASUEG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140634"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2767)
                     /locus_tag="VFX11_10175"
     CDS             complement(<1..2767)
                     /locus_tag="VFX11_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939032.1"
                     /translation="MVFSLTEIFGIGLGYLLFFFSVAWVTDQGYVPPRLVRHPFTYVL
                     SLGVFASAWAIYGAVGFAHDFGYNFLAYYLGISGAFMLAPIVLAPILRLVRNYQLGSL
                     ADLLSFRYRSPVVGALVTVCMLMGIMPLLAMQIQAVADSIHVLVPDTSPDMLAFSFVC
                     MIILFAILFGARHISAREKHEGLVVAIAFESLIKLVAMLVAGSAILYQVFGGFGGLND
                     WLSDHPDQLMQLYKPLEEGPWRSLVAAFFVSAIAMPHMYHMAFTENLNPRALLAASWG
                     LPLYLFLISLPILVIVWGASKLNIFGNPEYFTLSVTQSLHSPLLVILVFVAGLSAASG
                     VIIVCTLSLSAMVLNHLVLPLSPFNRRRNLYSWLLWTRRALIGAIILISYGFYLLMAG
                     RHTLSELGFLAFVAAMQFVPGLFGVLMWSRGNRDGFLAGLAAGFCIWLFGLLLPVLLG
                     VQIHVNIPILINDIFPTVNNSYMVATFAIFLNSLIFGIVSLLTEASASEKLAAETCNV
                     DNLRRPQRWELSVKTAREFVGRLAKPLGAVTARREIDLALNDLAMSLDESRPYALRRL
                     RDQLETNLSGLLGPSVAHDILESSLPYVIPSQNTGTQDIHFIESRLEEYHDKLTGLAA
                     ELDSLRRFHRQTLQDLPVGVCSLGSDGEVLGWNRSLELITRVPADTILGSHITHLPAP
                     WDELLKSFIEAPSRNQQREVDYEGVHRWLSLHKAALGLSKSNASPGGQVIVVEDTTEM
                     RMLEHHLAHSERLASVGRLAAGVAHEIGNPITGIACLAQNLRFDSEDPVVLETAEQIL
                     DQTKRITRIVQSLVSFSHSGSTHVAQEPVLIHESVQEAMDLLALNKESIQVRFINRTD
                     PSHCAKGDRQRLTQVFINLLSNARDASNPEFPITVRTRAEEHTIVTDVEDYGSGIPAE
                     LQERIFEP"
     gene            complement(2757..2933)
                     /locus_tag="VFX11_10180"
     CDS             complement(2757..2933)
                     /locus_tag="VFX11_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939033.1"
                     /translation="MYIDRLVLIFIAGAYVLSPALMQWWAESGAAWWRPFLVWGLLIG
                     LTFWIARSRDLDGI"
     gene            complement(3012..>3689)
                     /gene="gluQRS"
                     /locus_tag="VFX11_10185"
     CDS             complement(3012..>3689)
                     /gene="gluQRS"
                     /locus_tag="VFX11_10185"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX4939034.1"
                     /translation="VVYQSQRHARYGDAVQNLLQQGHAFYCTCSRKDLQALGTRAYPG
                     TCRGQRAKPPQEHAIRVQVENSDIHFDDALQGSCTFNLHDDGGDFIIKRKDGLYAYHL
                     AVVIDDAMQRITHIVRGSDLLDSTPRHIHLQRLLGLPTPHYAHLPVIVNAQGQKLSKQ
                     TFATALPRTDPVPALFACLQALGQQPPAQLQRATRDELLAWGIGHWSLARVPHCLSLS
                     EPSLARC"
BASE COUNT          728 a         1082 c         1242 g          637 t
ORIGIN      
        1 agggttcgaa gatgcgctcc tgcagctcgg cggggatacc gctgccgtag tcttccacgt
       61 cggtaacaat ggtgtgttcc tcggcgcggg tgcgaacggt gatgggaaac tcggggttgc
      121 tggcatcgcg ggcgttgctg agcaggttga tgaagacctg ggtcaggcgc tggcgatcac
      181 ctttggcaca gtggctggga tcagtccggt tgatgaagcg gacctggatg ctttccttgt
      241 tgagggccag caggtccatg gcttcctgca ccgattcatg gatcagcacc ggttcctgtg
      301 cgacgtgggt ggagccgctg tgactgaaac tcaccagcga ttgcacgatg cgggtgatgc
      361 gcttggtctg gtccaggatc tgctcggcgg tttccagcac gaccgggtcc tcgctgtcga
      421 agcgcaggtt ttgcgccagg caggcgatgc cggtgatggg gttgccgatt tcatgggcca
      481 cgcccgccgc cagccgccct accgatgcca ggcgttcgct gtgggccagg tggtgttcca
      541 gcatgcgcat ctcggtggtg tcctccacca caatcacctg accgccgggc gaggcgttgc
      601 ttttcgacag tcccagtgcc gccttgtgca ggctgagcca gcggtgtacg ccctcgtaat
      661 ccacttcccg ctgctggttg cggctgggtg cttcgataaa gctcttgagc agttcgtccc
      721 acggcgccgg cagatgggtg atatgggaac cgaggatggt gtcggcgggt acgcgggtga
      781 tcagttccag cgaacggttc cagcccagca cttcgccgtc gctgcccagc gagcacacgc
      841 ccaccggcag atcctgcagg gtttggcggt ggaaacggcg caggctgtcc agttcggcgg
      901 caaggccggt gagcttgtcg tggtattcct ccagccggct ttcgatgaaa tggatgtcct
      961 gggtgccggt attctgcgac ggaatcacat agggcagcga ggattcgagg atgtcgtggg
     1021 ccaccgacgg gcccagcagg ccggacaggt tggtttccag ctggtcacgc aggcgacgca
     1081 gggcgtaggg gcgggattcg tccaggctca tggcgaggtc gttcagggcc aggtcgatct
     1141 cgcggcgggc ggtgacggcg cccagcggct tggccagccg gccaacaaat tcccgcgccg
     1201 ttttcaccga cagctcccaa cgctggggtc ttcgcaagtt gtcaacgttg caggtttccg
     1261 ccgccagctt ttccgaggcg ctggcttccg tcagcagcga gacgatgccg aagatcagcg
     1321 agttcaggaa gatcgcgaag gtcgccacca tgtagctgtt gttgacggtg gggaagatgt
     1381 cgttgatgag gatgggaatg ttgacgtgta tctgcacgcc cagcagcaca ggcagcagca
     1441 ggccgaacag ccagatacag aagccggcgg cgaggccggc aagaaaaccg tcgcggttgc
     1501 cgcggctcca catcagcacg ccgaacaggc ccggcacgaa ctgcatggcg gcgacgaagg
     1561 cgaggaagcc cagctccgag agagtgtgcc ggccagccat cagcaggtag aaaccgtagc
     1621 tgatcaggat gatggcgccg atcagtgcgc ggcgggtcca cagcagccag gaatacaggt
     1681 tgcggcgacg gttgaaaggc gacagcggca gaaccaggtg gttgagcacc attgccgaca
     1741 gcgacagggt gcagacgatg atcacgccgc tggcggcaga cagccccgcc acgaacacca
     1801 ggatcaccag caggggcgag tgcagcgact gggtgacgct gagggtgaag tattccggat
     1861 taccgaaaat attcagcttg gacgcgcccc acacgatcac caggatcggc agggaaatca
     1921 ggaacaggta cagcggcagg ccccagctgg cggcaagcag ggcgcgcggg ttcaggttct
     1981 cggtgaaggc catgtggtac atgtgcggca tggcgatggc ggacacgaag aatgccgcca
     2041 ccagactgcg ccaggggcct tcctccagcg gcttgtacag ttgcatcagc tggtccgggt
     2101 gatccgacag ccagtcgttc aggccgccaa atccgccgaa cacctgatac aggatcgcgc
     2161 tgccggccac cagcatggcc accagcttga tcagggattc gaacgcgatc gccacgacca
     2221 gcccttcgtg tttttcccgc gccgagatgt ggcgggcacc gaacaggatt gcgaacagaa
     2281 tgatcatgca gacgaagctg aaggccagca tgtccggcga ggtatccggc accagcacat
     2341 gaatggaatc tgccaccgcc tgaatctgca tggccagcag gggcatgatg cccatcagca
     2401 tgcacacggt caccagcgcg cccaccacgg ggctgcggta gcggaacgac agcaggtctg
     2461 ccagcgatcc cagctggtag ttgcgcacca gccgcaggat cggcgccagc acaatgggcg
     2521 ccagcatgaa ggcgccggaa atgcccaggt aataggccag gaagttgtag ccgaaatcgt
     2581 gggcgaaacc cactgcgccg tagatggccc acgcgctggc gaacacgccc agcgacagca
     2641 cgtacgtaaa cggatgacgc acgaggcggg gcggcacata gccctggtcg gtcacccagg
     2701 ccacgctgaa gaaaaacagc agatagccca ggccaatgcc gaagatttcg gtaaggctaa
     2761 ataccatcga gatcgcggct ccgggcaatc cagaaggtga gtccgatcag cagaccccac
     2821 accaggaacg gccgccacca ggcagcgccg ctttccgccc accattgcat gagggcaggt
     2881 gacaacacgt aggcgccagc aatgaaaatc aggaccagtc gatcgatata catggaagga
     2941 ataccaccag aggctgcttg ctggatgtta cgtcagcggc gggggcagtg caaagttaag
     3001 gcgggccgga ttcaacagcg cgccagcgac ggttcgctca gtgacaggca atgcggcacc
     3061 cgcgccagcg accagtgtcc gattccccag gccagcaact catcgcgcgt ggcccgttgc
     3121 agttgtgccg gcggctgctg ccccagagcc tgcaggcagg caaacagtgc gggcaccgga
     3181 tccgttcgcg gtagcgcggt ggcaaaggtc tgcttcgaca gtttctgtcc ctgtgcattc
     3241 acgatcaccg gcaggtgggc gtagtgcggt gtgggcaggc ccagcagccg ttgcaggtga
     3301 atatggcgtg gcgtggaatc cagcaggtcg ctgccgcgca cgatgtgcgt gatgcgctgc
     3361 atggcatcgt cgatcactac ggccagatga taggcgtaga ggccgtcctt gcgcttgatg
     3421 atgaagtcgc cgccgtcgtc gtgcagattg aaggtgcagc tgccctgcag ggcgtcgtcg
     3481 aaatggatgt cgctgttttc cacttgcacg cggatggcgt gttcctgcgg cggttttgcg
     3541 cgttgtccgc gacaggtgcc cggatacgcc cgtgtgccca gcgcctgcag atccttgcgc
     3601 gagcaggtgc agtaaaacgc gtggccttgt tgcagcaggt tttgcaccgc gtccccgtag
     3661 cgggcatgac gctggctctg gtacaccac
//
LOCUS       DASUEG010000541         5926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_47158, whole genome shotgun sequence.
ACCESSION   DASUEG010000541 DASUEG010000000
VERSION     DASUEG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47158"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX11_10190"
     CDS             <1..377
                     /locus_tag="VFX11_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4939035.1"
                     /translation="MPALWSRVQVSLILLKKSDTFKSVIPSKLFESFAMNKPIILGVE
                     GESARIVEESRAGLCIEPENADALAQAVVRLADDRTAYAAMASCGKDYVERHFDRDVL
                     AQRFADAIHTTLVGEQTVSVQP"
     gene            526..2046
                     /locus_tag="VFX11_10195"
     CDS             526..2046
                     /locus_tag="VFX11_10195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX4939036.1"
                     /translation="MHSHSVPRIGGVAIVFAFLVPFVIWFPKTQEFAGIIAGVVLLFI
                     FGVWDDRRDISYRLKFGGQLLAAVAVVAVGDIRIEWIPFHSQPLESVWISWALTIFCL
                     LAITNAMNLADGLDGLAGGASILSFGLVGLLSYQSGSYPLVLTTCAVAGSILGFLRFN
                     SHPARIFMGDTGSQFLGFILGVQVILLTQKVNTAMSSALPLLILGLPVIDTASVMIRR
                     IVTGRSPFSPDKNHIHHRLLQMGLSHYETVVVLYFIQCAYIFLAYLLRFHVDAVVLSA
                     FALYSLTLLAVIQLALRGDWVRRHSVSMGTVGALLTGPFRQISQQSRLNNLLQVALVL
                     VVPAYSLYAVFSSDLLSPDIGVLAVMLALMMAVNIAAPSKRGWLDAIGMYTACALIAY
                     LGYQPERPAVWLLATDWFLVLLLGVAVWLSVMATSHRMFETSPLDFLVILLTILVPLL
                     FGQFERHAEILLAVVKFIVLLYAFEYIRHGDRLININLIRAVLMVCLGVIGLGAVI"
     gene            2176..4521
                     /locus_tag="VFX11_10200"
     CDS             2176..4521
                     /locus_tag="VFX11_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4939037.1"
                     /translation="MSAVMAACGGAEGRKQKYLDQAEASFAKQDYEKARISYKNVLQI
                     DPKDVKGREGYARTLERLKDWRGAVGQYRAIIEDDPKNNAAKIKLGQLYLLAKAPDLA
                     LTLAEEVLAVDAINDAALALKAGAQSQMGKSAEALVNAEQAYKLNKSSLDNIILLASI
                     YAGMKRQDDAIALLETETANHPASSSIYTMLSQIYLATQRTALAEGAMKKLIELNPSV
                     VGYKAQLARFYELSGRKDEAESILKAAVASDPAGVDSVIALHRFYVQRNDQEKAERVL
                     RDAIAVAPENHDLQLSLAGFFLSQKNIEKAREIYTALLEKDDATVLKAKTRLAYLSNL
                     EGKVDEALVTLEEVLKENPADVEALKLRGAINLARNKPVDAINDLRAVMSATPDAPDV
                     MKMLGRAHLLNRENQQAIDLYKAALNLQPGDVELRLQLADLYATMGEKNEAAKQLEAS
                     NRIAPNNAVILEKMVRTYIDSQYLDDAENVLEDLQKLDPQSPRTAYYQGVIYQGRNKH
                     QAALAEFDKSIALKPGASEPMAAKVKSYIALKQIDEAIAWLAGMEGGDQPNPVALNLQ
                     GELHMAKKSWDKARQYFQRCVDAKPEWWIPYRNRAMVESAEKNADSAYQYLAGVVDAV
                     DSAPLRIEMANYAERSGRYDSAIQQYESVLAKDADNQVIANNLAMLIVTYKKDAQSLN
                     RAKQLADSLSASDNPSFLDTAGWVQSVAGDYQRALPMIQQALRAAPDDPAIQYHLAMT
                     YLGASDKANAERYLERALESDRKFHGREEAEKILAQLKQQS"
     gene            4569..>5926
                     /locus_tag="VFX11_10205"
     CDS             4569..>5926
                     /locus_tag="VFX11_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939038.1"
                     /translation="MFRSIWLLMLALLMIQQVNAATFTDELKIGDRHDIETRSFALKA
                     GSHKFSIVDAFAGSSQKMASAQAFLVSGDRLLASVDLSRLNASFNLAADTEATLYVAG
                     KPASVHGLLLTRLERTGAAAPLLDEPFLFSKASEPLRTYEFEQTYILSKTAPVTLDLT
                     DFSASGKLPLTPFSSLLVLMTHRGDDGRFTYFYSDVASLEGGPELSHTIALSPGNLFI
                     QIVALAPDTGISQLGWSLKQDAVELAGDVFQIDDNASEPGVVVGEFNLAEPAVIDATA
                     AILSQQASDFSVAIASASNDDVVELSRQDLQVTSSLAAGRYKVLLLKDDNGSGLVGIE
                     IKSGAATLLATPVSLGDYEPLGELKLAAASSVDAGLHFFLLPAGLAGMDVLVANSQST
                     ALALNLSSMTQSGVSMAAGDYSVWVRSVAVSGEGYYRVHVQPQGGTTTQWWGALGGGL
                     IE"
BASE COUNT         1254 a         1571 c         1757 g         1344 t
ORIGIN      
        1 agatgccggc actctggtcc cgggtgcagg tatccctgat cctgctcaag aaaagcgata
       61 cgttcaagtc cgtcattcct tccaaactgt ttgaaagctt tgcaatgaat aagcccatca
      121 ttctcggcgt ggagggggag agcgcacgca tcgtggaaga atcccgggca ggtttgtgta
      181 tcgagccgga aaatgcggat gccctggcgc aggcggttgt tcgtcttgcc gacgatcgca
      241 ctgcgtacgc cgccatggca tcctgcggca aggactatgt ggagcgtcac tttgaccgtg
      301 atgtactggc acagcggttt gccgatgcca ttcacacaac actggttggc gaacagactg
      361 tttcggttca gccatgaatc cattgaatgg tatctgcgcc acgggatcac aatgccactg
      421 atcgccacgc tgatgacagc cttgttcata acagtggtca tgattccgta tctgatgcgg
      481 tatgcgggaa cgctcggtct ggtagatttg cccaacgaaa ggaaggtgca ttcgcactca
      541 gttccgcgca tcggtggtgt ggccatcgtt tttgcgtttc tggttccctt tgtcatctgg
      601 tttcccaaaa cccaggaatt tgcgggcatc attgccggcg ttgtgctgct gttcattttc
      661 ggcgtctggg atgatcgcag ggatatcagc tatcggctga agttcggcgg acaactgctg
      721 gcggcggtgg ctgtcgtggc ggtgggagac attcgcatcg aatggattcc gtttcattcg
      781 cagccgctgg aatcagtatg gatcagctgg gcgctcacca tcttttgcct gctggccatc
      841 accaatgcca tgaatctggc agacgggctg gatggactgg ccgggggggc gtcgattctc
      901 agtttcggcc tggtgggatt gctgtcctat cagtccggct cctatccgct ggtgctgacc
      961 acctgtgccg ttgccggcag catcctgggt tttctgcgtt tcaattcaca tccggcgcgc
     1021 attttcatgg gtgacactgg cagccagttc ctgggattca tcctgggtgt tcaggtcatt
     1081 ctgctgacgc agaaagtgaa caccgccatg agttcggccc tgccgctgct gattctggga
     1141 ttgccagtaa ttgatacggc ttccgtcatg atccggcgca ttgtcacggg gcgttcgcct
     1201 ttttcacccg acaagaatca cattcaccac cgtctgctgc agatgggcct ttcccactac
     1261 gaaaccgtgg tggtgctgta tttcattcag tgtgcgtata tttttctcgc ctatctgttg
     1321 cgctttcatg tggacgcggt ggtactgtct gcgtttgcgc tgtattcact gacgctgctt
     1381 gccgtcattc agctggcgtt gaggggtgat tgggtccggc gccactcggt cagcatggga
     1441 actgttggcg cactgctgac ggggccattc cggcagattt cccagcagtc acggttgaac
     1501 aacctgctgc aggttgcgct ggttctggtg gttccggcct attcgcttta cgccgtattc
     1561 agttcggatc tcctttcgcc tgatattggc gtgctggctg tgatgctggc actgatgatg
     1621 gcagtaaata ttgcggcgcc ttccaagcgg ggctggctgg atgccatcgg catgtatacg
     1681 gcctgtgccc tgatcgccta tctgggttac caacccgagc gaccggccgt ctggctgctg
     1741 gccacggact ggtttctggt gctgctgctt ggtgtcgcag tctggctctc ggtgatggcg
     1801 accagtcacc gcatgtttga aaccagtccg ctggattttc tggtgatact gctgaccatc
     1861 ctggtaccgc tgctgttcgg ccagttcgag cgccacgcgg aaattctgct ggcggtggtg
     1921 aaattcattg tcctgttgta tgccttcgaa tatatccgcc atggcgacag gctaatcaat
     1981 atcaacctga tcagagcggt gctgatggta tgcctgggcg tgatcggtct gggggctgtc
     2041 atttgaattc tgcgttctca caactgtaac ctttttgcag cattgctgtg acatagtttg
     2101 ccggcttcgt ctgcaattac cggaaacctg tcatttatgt gtattcgctt ttatcacctt
     2161 ctcctggttg tgctgttgtc tgcggtaatg gcggcgtgcg gtggcgccga gggccgtaaa
     2221 cagaaatacc tggaccaggc ggaagcaagc tttgcaaaac aggattatga gaaggcccgc
     2281 atcagttaca agaatgtgct gcagattgat ccaaaggacg taaagggccg cgaaggctat
     2341 gccagaacac tggagcgcct gaaggactgg cgcggcgctg tgggccagta ccgtgccatc
     2401 attgaagacg atccgaagaa caacgctgca aaaatcaagc tggggcagtt gtatctgctg
     2461 gcaaaggcgc ccgatctggc gctgacgctg gcagaagaag tgctggccgt cgatgcgatc
     2521 aatgacgcgg cactggcgct caaggccggt gcgcagagcc agatgggcaa gtcggcagag
     2581 gcgctggtca acgcggagca ggcctacaag ctgaataaat ccagtctcga taacatcatc
     2641 ctgcttgcgt ccatctacgc aggcatgaag cgtcaggatg atgccatcgc gctgctggaa
     2701 accgaaaccg ccaatcatcc tgccagttcc agcatctata ccatgctgag ccagatctat
     2761 ctggcgaccc agcgcaccgc gctggcagag ggtgcaatga aaaagctgat agagctgaat
     2821 ccgtcggtcg tcggttacaa ggcccagctg gcgcgctttt atgaactcag tggacgcaag
     2881 gatgaagccg aatccatcct gaaagctgcc gtggccagcg atcccgcggg cgtcgatagc
     2941 gtgattgcgt tgcatcgttt ttatgtgcag cgcaacgatc aggaaaaggc ggagcgtgtg
     3001 ctgcgtgatg caatcgcggt ggcacctgaa aatcatgatc tgcaactgag ccttgccggc
     3061 ttcttcctgt cgcagaaaaa tatcgagaaa gcccgtgaaa tctacacggc tctgctggaa
     3121 aaagatgatg caaccgtgct caaggccaaa acccgcctgg cctacctgtc caatctggaa
     3181 gggaaggtgg atgaagcact ggtgacactg gaagaggtgt tgaaggaaaa cccggcggat
     3241 gttgaggctc tcaaactgcg cggcgccatc aatcttgccc gcaacaagcc ggtggacgcc
     3301 atcaatgacc tgcgtgcagt aatgagtgca acgccggatg caccggatgt gatgaagatg
     3361 ctgggccggg ctcacctgct taaccgggaa aaccagcagg cgatcgatct gtacaaggca
     3421 gccctgaacc tgcagcccgg tgatgttgag ttgcggctcc agctggcgga cctgtatgcc
     3481 accatgggcg agaagaatga agctgccaag cagctggaag cctccaacag aattgcgccg
     3541 aacaatgccg tgatcctgga aaaaatggtg cgcacctata ttgattcgca gtatctggat
     3601 gatgcggaaa atgtactgga agacctgcag aagctggacc cgcaaagccc ccgcaccgct
     3661 tactaccagg gcgtgattta tcagggcaga aacaagcatc aggccgcatt ggcggagttc
     3721 gacaaatcaa ttgccttgaa acccggtgcc tccgaaccca tggccgccaa ggtgaagagc
     3781 tacattgcgc tcaagcagat cgatgaagcg atagcctggc tggccggcat ggaaggtggc
     3841 gatcaaccca atcctgttgc actcaacctg cagggtgagt tgcatatggc caagaaatcc
     3901 tgggataagg cacggcagta ttttcagcgc tgcgtcgacg ccaaaccaga atggtggatt
     3961 ccctaccgca atcgcgccat ggtggagagt gccgagaaga acgccgacag cgcctaccag
     4021 tatcttgcag gagtagtcga tgccgtggat agcgcaccat tgcgtatcga gatggccaac
     4081 tacgcggaac gcagtggccg gtacgacagc gccatccagc agtatgaatc tgtactggca
     4141 aaagatgccg acaatcaggt tatcgcgaac aatctggcga tgctcatcgt aacctacaag
     4201 aaagatgcgc agagcctgaa tcgcgccaag cagctggcag acagcctgtc agcttccgac
     4261 aatccatcat tcctggatac ggcgggctgg gtgcagtcgg ttgcgggtga ttatcagcgc
     4321 gcgctgccca tgatccagca agcgctgcgg gctgcgccgg atgatccagc tatccagtat
     4381 caccttgcca tgacctatct tggcgccagt gacaaggcca atgccgagcg ctatctggaa
     4441 agagcgcttg aaagcgacag gaaattccat ggtcgggaag aagcggaaaa aattcttgca
     4501 caattgaagc agcagagctg agtagttggg ggttgcaaat gaatacggga atgaaaacag
     4561 gaacagcagt gtttcgatcg atatggttat tgatgctggc gttgctgatg attcagcagg
     4621 taaatgccgc cacctttacg gacgaattga agatcggtga cagacacgat attgaaaccc
     4681 gctctttcgc actgaaggcc ggatctcaca aattcagtat tgtcgatgcc ttcgctggtt
     4741 ccagccagaa gatggcgtcg gcgcaggcat ttctggtcag cggtgatcgc ctgctcgcca
     4801 gcgtcgatct gtcacggctg aatgccagct ttaaccttgc cgccgatacc gaggccaccc
     4861 tctatgtagc aggcaagccg gcctcagtac acggattgct gctgacacgg ctggagcgga
     4921 ccggcgcagc cgcaccattg ctggatgagc ctttcctgtt cagtaaagcg tccgagccct
     4981 tgcgcacata tgaattcgag caaacctaca ttctcagcaa aaccgcaccc gtcacgctgg
     5041 atctgaccga ttttagtgcc tctggtaagt tgccgctgac acctttcagc agtctgctgg
     5101 ttctgatgac gcaccgtggc gatgatggcc ggtttaccta tttctattcg gacgttgcct
     5161 ctcttgaagg tggacccgaa cttagccaca ccattgcgtt gtcgcctgga aatctcttca
     5221 ttcagatcgt tgcactggcg ccggacacgg gtatttcgca gcttggctgg tcgctgaagc
     5281 aggatgcagt tgagctggcc ggagatgtgt tccagattga cgacaacgcc tctgagccgg
     5341 gcgtggtggt tggcgaattc aatctcgccg agccggccgt cattgacgct acggccgcca
     5401 ttctgtcgca gcaggccagc gatttttccg tggctatcgc ctcggccagc aatgatgacg
     5461 tggtggaatt gagccggcag gatctgcagg tcaccagcag tctggcggcg ggtcgttaca
     5521 aggtgctgct gctcaaggac gacaacggca gcgggctggt cggcattgaa ataaagtccg
     5581 gtgcggcaac attgctggcg actccggtgt cactgggcga ttacgagccg ctgggtgaac
     5641 tcaaactggc agccgcaagc agcgttgatg caggcctgca tttcttcctg ctgcctgcgg
     5701 gcctggctgg catggacgtg ctggttgcca atagccaatc gacggcactg gcattgaatc
     5761 tttccagcat gacgcaaagt ggtgtcagca tggctgccgg cgattattcg gtatgggtcc
     5821 ggtccgtggc tgtcagcggc gaaggttatt accgggttca cgtccagccg caaggcggaa
     5881 ccacgacgca atggtgggga gcgctgggcg gtggcctgat tgagag
//
LOCUS       DASUEG010000542         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_113651, whole genome shotgun sequence.
ACCESSION   DASUEG010000542 DASUEG010000000
VERSION     DASUEG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113651"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /gene="tsaE"
                     /locus_tag="VFX11_10210"
     CDS             complement(<1..286)
                     /gene="tsaE"
                     /locus_tag="VFX11_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX4939039.1"
                     /translation="MSELHWTLPAEADTVALGQRLAAVLAKGGVLYLQGDLGAGKTTL
                     SRGLIQALGHSGAVKSPTYTLVEPYELSCGRLYHFDLYRLNDPEELELIGI"
     gene            complement(293..1843)
                     /locus_tag="VFX11_10215"
     CDS             complement(293..1843)
                     /locus_tag="VFX11_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX4939040.1"
                     /translation="MSQATSPQYTSECPADLYRAAGVRELDRVAIQEQGIPGFTLMSR
                     AGAAAFALLRERWPQGRKITVFCGVGNNGGDGYVIAALAQQAGLQPQVIQVGDAGRSQ
                     GDALTALQQAQQGGVPFQLFAPGSIDAQLADTDVIVDALLGTGLSGAVRPDYALAIDA
                     INRAGLPVLAVDIPSGLCADTGSILGCAVKAVATITFIGMKQGLLTGEAPACTGALFF
                     AGLDVPAIVYRAVPPSATLVRQNHIKEWLPPRSRLSHKGNNGHVLVVGGDAGMGGAVA
                     MAAEAAGRVGAGLVSVVTRPEHIAPILARRPECMVTSQTDLGPLLQKATVVVIGPGLG
                     QQEWGQSLLRQALASPLPLVLDADGLNLLALWQEQGDSLARRGNGVITPHPGEAARLL
                     RAHPAYASTAQIQRNRFDTVRALQQQYAAVAVLKGAGSLIAGTDGRVWLSNTGNPGMA
                     SGGMGDVLSGVIGGLIAQGLTLEQAAVAGVWMHGAAADKAAAQGGERGLLATDLMSWL
                     RHWANGSG"
     gene            1934..>2608
                     /gene="queG"
                     /locus_tag="VFX11_10220"
     CDS             1934..>2608
                     /gene="queG"
                     /locus_tag="VFX11_10220"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX4939041.1"
                     /translation="MPPDLDYTALAHQIKAWGEALGFQKIAVTDVDLGEHEHYLQRWL
                     ELGYHGEMEYMARHGSKRSRPAELQPGTLRVITARMDYLAPDPQIVEVLNDPEKAFVS
                     RYALGRDYHKLIRRRLTQLGEKITAAIGPFDYRAFVDSAPILEKALAEKSGLGWIGKH
                     TLLLDRSAGSWFFLGEIYTDLPLPVDEPVTKHCGQCRACIDICPTQAIVAPYQLDARK
                     CISYLTI"
BASE COUNT          460 a          879 c          818 g          451 t
ORIGIN      
        1 ggatgccgat cagttcgagt tcctcgggat cgttgaggcg ataaaggtcg aaatgataga
       61 gccggccgca ggacagttca tagggctcca ccagggtgta ggtgggactt ttcactgcgc
      121 cggaatggcc cagcgcctgg atcaggcccc ggctgagcgt tgttttgccc gcccccagat
      181 cgccctgcag gtagagtacg ccgccttttg ccagcacggc tgccagccgc tggcccagtg
      241 caaccgtgtc cgcctcggcg ggaagtgtcc agtgcagttc gctcatgtcg cttcacccgc
      301 tgccgtttgc ccaatgccgc agccaggaca tcaagtccgt ggccagcagc ccccgttccc
      361 caccttgcgc cgcagccttg tccgccgctg cgccgtgcat ccagacaccg gctaccgccg
      421 cctgttccag agtcagcccc tgggcgatca ggccgccgat cacgccgctg agcacatcgc
      481 ccatgccgcc gctggccatg ccggggttgc cggtattgga cagccagacc cggccatcgg
      541 tgccggcgat caggctgccc gcgcctttga ggacggcaac ggcggcgtat tgttgttgaa
      601 gcgctcgcac ggtgtcaaac cggttgcgct gtatttgcgc ggtgctggcg taggctggat
      661 gggcacgcag tagccgcgcc gcctcacctg gatggggcgt gatcaccccg ttgccgcgtc
      721 gagccaggga atcgccctgt tcctgccaga gggccagcag gttgagtccg tccgcgtcca
      781 gcaccagtgg cagcgggctg gccagcgcct gtcgcagcag cgactgtccc cattcctgct
      841 gacccagacc gggaccgatg acgaccactg tcgccttctg cagcagcgga cccagatcgg
      901 tctggctggt gaccatgcac tccgggcgcc gcgccaggat gggggcaata tgctcgggac
      961 gggtgaccac tgacaccagt cccgcgccca cgcggcctgc ggcctccgcc gccatcgcca
     1021 cggcaccgcc catgcccgcg tcgccaccga cgaccagcac atggccgttg ttacccttgt
     1081 gggaaaggcg ggaacggggc ggcagccatt cctttatatg gttttgtcga accaaggtgg
     1141 cgctgggggg aaccgcgcga tacacgattg ccggtacgtc gaggccggcg aaaaacagcg
     1201 cgccggtgca ggcaggggcc tcgccggtga gcaggccctg cttcatgccg atgaaggtga
     1261 tggtggcgac ggccttgacc gcacagccga gaatgctgcc ggtatcggcg cagaggccgg
     1321 aggggatgtc cactgcaagg acaggcaggc cagcgcggtt gatggcatcg atggcgaggg
     1381 cgtagtccgg gcgcacggcg ccggacagac ctgtgcccag caacgcatcg acaatcacat
     1441 cggtatcggc cagctgcgcg tcaatgctgc caggggcaaa cagctggaac ggcaccccgc
     1501 cctgctgcgc ctgctgcaat gccgtcagcg catcgccctg gcttctgcca gcatcgccca
     1561 cctggatcac ctgcggctgc agccccgcct gctgcgccag tgccgcgatg acatagccat
     1621 cgcctccatt gttgccgaca ccgcaaaaca ccgtgatttt ccgcccctgc ggccaacgtt
     1681 cccgcagcaa cgcgaaggca gcagcaccgg cccggctcat cagcgtgaag cctggaatgc
     1741 cctgttcctg gatcgccacc cggtccagct cgcgcacacc cgcggcgcgg taaaggtccg
     1801 ccggacattc gctggtatac tggggcgacg ttgcttgcga catggatttt tccgtcattg
     1861 gatcttgcac ctgcagttcc ggcatcgatc agattgttat atcacagaca ccgccccatg
     1921 aggttccccc gtgctgcccc ccgaccttga ttacaccgcg ctggcgcacc agatcaaagc
     1981 ctggggcgag gcgctggggt ttcagaaaat cgcggtgacg gacgtggatc tgggtgaaca
     2041 cgagcattat ctgcaacgct ggctggaact gggttatcac ggcgagatgg agtacatggc
     2101 gcggcacggc agcaagcgtt cgcgccctgc cgaactgcag cccggcacgc tgcgggtgat
     2161 caccgcgcgc atggattacc tggcgccgga cccgcagatt gttgaagtgc tgaatgatcc
     2221 ggaaaaggcc tttgtttccc gctacgcact gggccgcgac taccacaagc tgatccgccg
     2281 gcggctgacg caactgggag aaaaaatcac ggccgcaatc ggcccgttcg actaccgcgc
     2341 gttcgtggac agcgccccta ttctggaaaa agccctggca gaaaaatccg gcctgggctg
     2401 gatcggcaag cacacgttgc tgctggaccg cagtgccggc tcctggtttt tcctgggcga
     2461 aatctacacg gatctgccac tgccggtgga cgaaccggta accaagcatt gcggccagtg
     2521 ccgcgcctgc atcgacatct gccccaccca ggcgatcgtg gcgccttacc agctcgacgc
     2581 gcgtaaatgc atttcctacc tcaccatc
//
LOCUS       DASUEG010000543         6418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1161972, whole genome shotgun sequence.
ACCESSION   DASUEG010000543 DASUEG010000000
VERSION     DASUEG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161972"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /gene="murF"
                     /locus_tag="VFX11_10225"
     CDS             <1..1339
                     /gene="murF"
                     /locus_tag="VFX11_10225"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX4939042.1"
                     /translation="IGSDGEFSSVSTDTRTLQQGDLFVALQGPNFDGHTYLPQAFAKG
                     AVAALVSKPCDVPQPQILVADTLRGLGDTGRVWAQQFAVKKIAITGSSGKTTLKEMVA
                     AILAEVAETLSTKGNLNNDIGVPLTLSRLNAQHRFAVVECGANHVGEIAYTVNLVQPD
                     VAVVNNVAPAHLEGFGSIDRVAQAKGEIYSGLGVHGVAVINLDDAYAHQFLAQTSKLR
                     QLTFSLRNPQASVVADNLRCNALGQYAFTVQLPDDQFDIQLPLLGVHNVRNALAACAL
                     TLALDIPVAAMLAGLKKVRAVPGRLFPVEDLPGFNIIDDSYNANPASLKAAIDVLADV
                     SSSTCLVLGGMGELGDTSAQLHEQVGSYAAQKGILRVYSLGPNAGFYQCGYVQHQPPQ
                     GRFIVCDSHQQIADNLKQHEKDKTILVKGSRSTAMEKVIHALRGCDHLREETH"
     gene            1339..2421
                     /gene="mraY"
                     /locus_tag="VFX11_10230"
     CDS             1339..2421
                     /gene="mraY"
                     /locus_tag="VFX11_10230"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4939043.1"
                     /translation="MLLWVAEYLKQDASFFNVFQYLTLRGILGVLTALSIAFIVGPTM
                     IRKLSFHQIGQSVRNDGPKSHLSKAGTPTMGGALILVAIFISTLLWADLRNRYVWVVL
                     AITFIFGAVGWVDDYRKVVQRNSKGLPAKWKYFWQSVGGLGVAFFLYYTAQTPAETQL
                     LVPFFKNVQVDIGWFYIVLTYFVIVGTSNAVNLTDGLDGLAILPTVLVGGALGVFAYV
                     TGNVQFATYLQFPYIAGTGEMIVICGALVGAGLGFLWFNTYPAQVFMGDVGALALGGI
                     LGTLAVITRQEIVLFIMGGVFVMETVSVILQVASFKLTGRRIFRMAPIHHHFELKGWA
                     EPKVIVRFWIITVILVLIGLATLKLR"
     gene            2466..3815
                     /gene="murD"
                     /locus_tag="VFX11_10235"
     CDS             2466..3815
                     /gene="murD"
                     /locus_tag="VFX11_10235"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4939044.1"
                     /translation="MSRLAGKYVVIGLGKTGLSCVRFLKHLGCDVAVVDTRAVPPGVH
                     ELKAEFSDVPFYSGELESFDFTTAQELIVSPGLSIRTPSIARAQAAGVSIAGDIELFC
                     RTISAPVIAVTGSNGKSTVVTLLGKLLESTGQKVKVAGNIGLPVLDAILAKEEVDVWV
                     LELSSFQLETTQSLRAQVACILNVTEDHMDRYDSINDYASAKQRIYDGCDTAVYWQDD
                     ARTRPVGQTPKLVAFGGGIEKHGRFHVRQVQGGQVMDGDQIVLNASELRIKGAHNLLN
                     VAAILAIIDAFGMDYHAVLPVLREFPGLSHRCQWVANSNGVDWYNDSKGTNVGAAEAA
                     IEGLGTSISGKLVWLAGGDGKGADFSPLRPSVQKHVRQAVLFGRDAQLIAASIKGACA
                     VEFATSLADAVMKAGAVAQAGDAVLLSPACASFDMFKNYEDRGDQFVAAVKDRMVTQ"
     gene            3812..4993
                     /gene="ftsW"
                     /locus_tag="VFX11_10240"
     CDS             3812..4993
                     /gene="ftsW"
                     /locus_tag="VFX11_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX4939045.1"
                     /translation="MSQMAIPLNSNALSRRQWLLLFSVILALIGCVMVASASMDMAAI
                     NYKSPFYFVERQAVFLLAGVMAGIAVYQVPLAWWERSSVPLLLVAYVLLVLVLLPGVG
                     RTVNGSTRWLVLGPINVQVSEIVKICALMYVAGYLVRRTEEVRERFSGFMKPVIILSI
                     MVAFLMKQPDFGAVVVIMTSVFVMLFLAGVKFGQFSLVVLGSVAAGALMVYSSEYRMR
                     RLLGYIDPWAEQYASGYQLVQSLIAFGRGEWFGVGLGNGIQKLFYLPEAHTDFVFAVL
                     AEEFGLVGNLVVIAIYMALVFTAMSIGRAAEKQGKRFNGYLVYGLSMMLGLQALVNVG
                     VSSGLLPTKGLTLPLISYGGSSLIIVCVVLALIMRADYENNHNAERKAAKRTMDLEVT
                     G"
     gene            4990..6087
                     /gene="murG"
                     /locus_tag="VFX11_10245"
     CDS             4990..6087
                     /gene="murG"
                     /locus_tag="VFX11_10245"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX4939046.1"
                     /translation="MNAPRKVQRVLVTAGGTGGHVFPALAAAKMFQEQGVEVRWLGTA
                     QGIEATVVPANNIAIDYLDVSGVRGQGLVRLLLAPLKIVRSVLRVMQLIRQHKPDAVL
                     GMGGFVTGPTGVGAWLAGCPLFIHEQNAIAGFTNRVLAKFAKRVFQAFPATFAASSKV
                     ETTGNPVRREIAAVEAPDTRYDKHTGPLRFLILGGSQGAVALNERVPQALAQLKGKLD
                     FVVKHQAGAKNIKTAERFYKEANVNADVIPFIDDMAAAYGWADAVICRSGALTVSELA
                     AAGVAALLVPYPHAVDDHQTRNGEYLSRNGAAKLVQQKDMTTESLADLLLQSFADRQQ
                     LKQMAVKARALAMTDATRKVVQQCLEVASGK"
     gene            6137..>6418
                     /locus_tag="VFX11_10250"
     CDS             6137..>6418
                     /locus_tag="VFX11_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007242933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX4939047.1"
                     /translation="MRRIRSIHFVGIGGAGMSGIAEILHNLGYAVSGSDRSDSATLRR
                     LAALGLRTFVGHAAAQQAHADAVVVSSAIGADNPELVAARARRVPVVPRA"
BASE COUNT         1210 a         1699 c         2120 g         1389 t
ORIGIN      
        1 gatcggcagc gacggggaat tttccagtgt ctccaccgac acgcgcacgc tgcagcaggg
       61 cgatctgttc gtggcgttgc aggggccgaa ttttgatggc cacacctatt tgccgcaggc
      121 ctttgccaaa ggtgcggtgg cggcgctggt gagcaagccc tgtgatgtgc cgcagccgca
      181 gattctggtg gcggacactc tgcgtggcct gggtgataca ggccgggtgt gggcgcagca
      241 gtttgcggtg aagaaaattg cgattaccgg cagcagtggc aaaaccaccc tgaaggaaat
      301 ggtggcggcg attctggcgg aagtggctga aaccctgtcc accaaaggca acctgaacaa
      361 tgacattggc gtgccgttga ccttgagccg gctcaatgcg cagcaccgtt ttgccgtagt
      421 cgaatgtggt gccaatcacg tgggcgaaat tgcctacacc gtgaatctgg tgcagccgga
      481 tgtggcggtg gtgaacaatg tggcacctgc acatctggaa ggatttggtt ccatcgaccg
      541 ggtggcgcag gccaaaggcg agatatacag cggtctgggc gtgcacggcg ttgctgtgat
      601 caatctggat gatgcctatg cccaccagtt tctggcgcag accagcaagc tgcgccagct
      661 gacgttctct ctgcgcaatc cgcaggccag cgttgtcgcg gacaacctgc gctgcaatgc
      721 gctgggacag tatgcgttta ccgtgcagct gccggatgac cagtttgaca tccagttgcc
      781 gctgctgggc gtgcacaacg tgcgcaatgc gctggctgcc tgcgcgctga ccctggcact
      841 ggatattccg gttgccgcca tgctggcggg cctcaaaaaa gtacgtgcgg tgcccggtcg
      901 cctgttcccg gtggaagacc tgccgggctt caacattatc gatgacagtt acaacgcaaa
      961 tcctgcttct ctcaaggctg caatcgatgt gctggccgat gtgagcagca gcacctgtct
     1021 ggtgctgggc ggcatgggtg aactgggtga caccagcgca cagctgcatg aacaggtggg
     1081 gtcttatgcc gcgcagaaag gcatcttgcg ggtttacagc ctgggcccga atgccggatt
     1141 ttatcaatgc ggttatgtac aacatcagcc gccgcaggga cgcttcattg tgtgcgacag
     1201 ccaccagcag atcgccgaca acctgaaaca acacgaaaaa gacaaaacca ttctggtcaa
     1261 gggttcacgc agtaccgcca tggaaaaagt gatccatgcc ctgcgcggtt gtgaccattt
     1321 gcgcgaggaa acacactgat gctgctttgg gtggcggaat acctgaaaca agatgcttcg
     1381 tttttcaatg tctttcagta tctgacgttg cgtggaatcc tgggtgtgct gacggcactg
     1441 tcgattgcgt tcattgtcgg gccgaccatg attcgcaaac tcagctttca ccagatcggc
     1501 cagtcggtac ggaacgatgg ccccaagtcc catctgagca aggccggcac gcccaccatg
     1561 ggtggtgcgc tgattctggt ggcgatattc atcagtacgc tgctgtgggc ggatctgcgc
     1621 aatcgttatg tctgggtcgt gctggcgatc acgttcattt ttggcgccgt gggctgggtc
     1681 gatgattacc gcaaggtggt gcagcgcaat tcgaaaggtc tgccggcgaa gtggaaatat
     1741 ttctggcagt cggtgggcgg actgggcgtg gccttcttcc tttattacac cgcgcagact
     1801 ccggcggaaa cccagttgct ggtgccgttc ttcaagaatg tacaggtgga tatcggctgg
     1861 ttctatatcg tgttgaccta cttcgtgatc gtgggtacct ccaacgcggt gaatctgacc
     1921 gatggcctgg atggtctggc gatcctgcca acagtgctgg tgggcggcgc actgggcgtg
     1981 tttgcgtacg ttacaggtaa cgtgcagttc gccacctatc tgcaatttcc ctatatcgcc
     2041 ggcaccggcg aaatgatcgt gatctgcggt gcgctggtgg gggccgggct gggttttctc
     2101 tggttcaaca cctatccggc acaggtgttc atgggcgatg tgggcgcgct ggcgctgggc
     2161 ggtattctcg gtacgctggc ggtgattacc cgtcaggaaa tcgtgctgtt catcatgggt
     2221 ggtgtgttcg tgatggaaac cgtgtcggtg atcctgcagg tggcatcgtt caagctgacc
     2281 ggtcgccgca ttttccgcat ggcgccgatt catcaccatt ttgaattgaa aggctgggca
     2341 gaaccaaaag tcattgtccg tttctggatc atcactgtca ttctggtgct gattggactg
     2401 gcaaccctga agctgcgtta ggaagaagtg ggattgggag ctaagtggtc ttgggagaaa
     2461 gacgcgtgag tcgactggca ggaaagtatg tagtaatcgg actcggcaag accgggttgt
     2521 cctgcgtgcg ttttctcaag catctggggt gtgatgtggc cgtggtggat acccgcgccg
     2581 tgccaccggg tgttcatgag ctgaaggcgg aattttccga tgtgccgttt tattccggcg
     2641 agctggaatc tttcgatttc accactgcgc aggaattgat tgtcagtccg ggtctgtcga
     2701 tccgcacacc atccatcgcc cgcgcacagg cagcgggtgt gtcgattgcc ggcgatatcg
     2761 aacttttttg ccgcacgatc agcgcgccgg tgattgcagt gaccggttcc aacggtaaaa
     2821 gcacagtggt cacgctgctg ggcaagctgc tggagtccac cgggcaaaaa gtgaaagtgg
     2881 cgggcaatat tggcctgccg gtgctggatg cgattctggc caaggaagaa gtggatgtgt
     2941 gggtgctgga gctgtcgagt tttcagctgg aaaccaccca aagcctgcgt gcgcaggtcg
     3001 cctgcattct gaacgtgacc gaagatcaca tggatcgcta cgactcgatc aacgattacg
     3061 ccagcgcgaa gcagcgcatc tatgacggtt gtgatacggc cgtttactgg caggacgatg
     3121 cgcgcacccg tccggtgggg cagacgccga agctggtggc atttggcggt ggtattgaaa
     3181 agcacggccg cttccatgtg cggcaggtgc agggcggcca ggtcatggat ggcgaccaga
     3241 ttgtgctgaa cgccagcgag ctgcgcatca agggcgcaca caatctgctg aacgtggcgg
     3301 cgattctggc gatcatcgat gcattcggta tggattacca cgctgtgttg ccggtgctgc
     3361 gggagtttcc cggtttgtcg catcgctgcc agtgggtggc gaacagcaat ggcgtcgact
     3421 ggtacaacga ttccaagggt acgaatgtcg gtgcagcgga agcggccatc gaggggctgg
     3481 gcaccagtat cagcggcaag ctggtgtggc tggcgggtgg cgacggcaag ggagccgatt
     3541 tttcgcccct gcggccatca gtgcagaagc atgttcgcca agcagtgttg ttcgggcgcg
     3601 atgcacaatt gattgcggcc agtatcaagg gcgcgtgtgc ggtggaattt gccacctcgt
     3661 tggcggatgc agtaatgaaa gccggcgccg ttgcgcaagc cggtgacgcg gtactgctgt
     3721 ctcctgcctg cgccagtttc gatatgttca agaactatga agatcgcggt gaccagtttg
     3781 ttgccgcagt gaaagacagg atggtgacgc aatgagccag atggcgattc cgttaaacag
     3841 caacgcactg tcgcgtcggc agtggctgtt gctgttttcg gtgatcctgg cgctgatcgg
     3901 ttgcgtgatg gtggcgtcgg cctcgatgga tatggcggcg atcaactaca agtcgccgtt
     3961 ctatttcgtc gagcgccagg ctgtttttct gctggcgggc gtgatggccg gtattgctgt
     4021 ataccaggtg cccctggcct ggtgggaacg cagcagtgtg cccttgctgc tggtggcgta
     4081 tgtattgctg gtgctggtgc tgctgcccgg tgtcggccgc acggtgaatg gcagtacccg
     4141 ctggctggtg ctgggcccga tcaacgtgca ggtgtcggaa atcgtgaaaa tctgcgccct
     4201 gatgtatgtg gccggttatc tggtgcggcg taccgaggaa gtgcgcgagc gtttttccgg
     4261 attcatgaaa ccggtgatca tcctgtcgat aatggtagcg ttcctgatga agcagccaga
     4321 cttcggcgcg gtggtggtga tcatgacctc ggtgttcgtc atgctgtttc tggcgggcgt
     4381 gaagttcggc cagttttcgc tggtggtgct gggcagcgtg gcggcgggcg cattgatggt
     4441 gtactcgtcg gaataccgca tgcggcgtct gctgggctat atcgatccct gggcggaaca
     4501 atatgcgtcc ggttaccagc tggtgcagag cctgattgca ttcgggcgcg gtgaatggtt
     4561 cggcgtcggc ctcggcaacg gcatccagaa actgttttat ctgccggaag cgcacacgga
     4621 tttcgtgttc gccgtgctgg cggaggaatt tggtctggtg ggcaatctgg tggtgatcgc
     4681 gatttacatg gcactggtgt ttaccgccat gtccatcggt cgggcggcgg aaaagcaggg
     4741 caaacgtttc aacggttatc tggtttatgg tttgagcatg atgctgggat tgcaggcgct
     4801 ggtgaatgtg ggcgtcagca gcggcctgtt gcccaccaag gggctgactt tgccgctgat
     4861 cagttacggt ggcagcagtc tgatcatcgt ctgcgtggtg ctggcgctga tcatgcgcgc
     4921 cgattacgag aacaatcaca acgcagaacg caaggccgcc aaacgcacga tggatctgga
     4981 ggtgacagga tgaacgcgcc tcgcaaggtc cagcgggtat tggtaacagc gggcggcacc
     5041 ggcgggcacg tgtttcctgc cctggcggct gcaaaaatgt ttcaggagca gggcgtggaa
     5101 gtgcgctggc tgggcaccgc gcaaggcatc gaagcgaccg tcgttcccgc caacaacatt
     5161 gccatcgact atctggacgt gtccggcgtg cgtggacagg gactggtgcg gttactgctg
     5221 gcgccgctga aaatcgtccg ctccgtgctg cgggtaatgc agctgatccg tcagcacaaa
     5281 ccggacgcag tgttgggtat gggtggtttc gtcaccggtc ccaccggcgt tggcgcctgg
     5341 ctggcgggtt gtccgctgtt catccatgag cagaatgcga ttgccggttt caccaatcgc
     5401 gtgctggcga aattcgcaaa acgggtattt caggcgtttc cggcgacctt tgccgcttcg
     5461 tccaaggtgg aaaccaccgg caatccggtg cgcagggaaa tcgccgccgt cgaagcgccc
     5521 gacacacgct acgacaaaca cacgggaccg ctccgttttc tgattctcgg cggcagccag
     5581 ggcgccgtgg cgctgaacga aagggtgccg caggcgctgg cacagttgaa aggcaaactg
     5641 gattttgtgg tgaagcatca ggccggcgcg aaaaatatca aaaccgccga gcgcttctac
     5701 aaggaagcga atgtgaacgc ggacgtgatt ccgttcatcg acgatatggc cgctgcttat
     5761 ggctgggcag atgcagtgat ctgccgctcc ggtgcgctga ccgtgagcga actggccgcc
     5821 gctggcgtcg ccgcactgct ggtgccttac ccccacgcgg tggacgacca ccagacccgc
     5881 aacggcgaat acctcagccg caatggcgcc gcgaaactgg tgcagcagaa agacatgacg
     5941 acggaatccc tggcggacct gctgctgcag tccttcgccg accgtcagca actcaaacag
     6001 atggcggtca aggcccgtgc gctggccatg accgatgcca cccgcaaagt ggtgcaacag
     6061 tgcctggagg tggccagtgg aaaataagcc ggtggaaaac agaaaagtct cgacccggat
     6121 gatccagatt cccgaaatgc ggcggattcg cagtattcac ttcgtcggca tcggtggcgc
     6181 cggcatgagc ggcatcgccg agatcctgca caacctgggt tatgcggtgt cgggttccga
     6241 ccggtccgac agcgccacgc tgcggcgcct ggccgcgctc gggctgcgca ccttcgtcgg
     6301 gcacgccgcg gcgcagcagg cgcacgccga cgcggtggtc gtgtcgagcg cgatcggggc
     6361 cgacaacccg gagctcgtcg cggcgcgcgc gcgccgcgtg ccggtggtgc cgcgcgca
//
LOCUS       DASUEG010000544         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1741731, whole genome shotgun sequence.
ACCESSION   DASUEG010000544 DASUEG010000000
VERSION     DASUEG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741731"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..881
                     /locus_tag="VFX11_10255"
     CDS             213..881
                     /locus_tag="VFX11_10255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4939048.1"
                     /translation="MSRTMDAQRERLPGLIRSDYRQTAADTGLAQPSDAVLHALQEVP
                     RHLFVQKAFQDSAYSNSPLPIGNGQTISQPFIVALMTDLLLLKPSDKVLEIGTGCGYQ
                     SAILSLLAKDVYSLEIVSDLYKQSTERLAGLGYDNVHVRLGDGYYGWQEQAPFDAILV
                     AATADALPEPLLVQLAPGGRMVIPVRDASGREVLQLVTKDAAGQVQVRDTIGVRFVPL
                     TGEH"
     gene            complement(904..2193)
                     /locus_tag="VFX11_10260"
     CDS             complement(904..2193)
                     /locus_tag="VFX11_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HEX4939049.1"
                     /translation="MQHYPVFIVGGGQAGLSISHYCQQHNIGHLVAEKHRLMHAWKEK
                     RWDSFTLVTPNWQCQLPDHPYTGDDPHGFMKRDEIIDYLNAFERKVDAPVLEHTTVTR
                     IKHDGERYRIETTQGSFSADQVVVASGSYQTPVIPRYAERLPRTIRQLHSEQYRNPGQ
                     LGDGAVLVVGSGQSGAQIAEDLHLAGKRVMLAVGDAPRCARFYRGRDVVAWLTDMKYY
                     DMPVHEHPLREGVRDNTNHYVTGRDGGRDIDLRKFALEGMELYGKILDVDGTHLRFSP
                     NLAAVLDSADKTYNGINERIDQFIAANSIDAPPASRYQPVWQPPQERETLDLDSANIT
                     DVIWCIGFAPDYHWLDIPVFNGRGYPAHQRGVTPFPGLYFIGLPWLYTWGSGRFSGID
                     QDAKYVVEVIAQQLAQAGKLTSPPKVKMNPCLAAALG"
     gene            complement(2225..2506)
                     /locus_tag="VFX11_10265"
     CDS             complement(2225..2506)
                     /locus_tag="VFX11_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HEX4939050.1"
                     /translation="MPETYFDVRWPDGHEETCYSPSSVVADFFQPATTYPLADFLARS
                     EDALMLASERVRQKFGYSCSSAADQLQRIRQTAEQFSGQGAVTVLRIHS"
     gene            complement(2487..3461)
                     /locus_tag="VFX11_10270"
     CDS             complement(2487..3461)
                     /locus_tag="VFX11_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HEX4939051.1"
                     /translation="MSLTELLDTVRSSAGLHCKTEIQLVQRHLAEAYPAPFPNGDDAA
                     VIPSGDGFDLLAGEGFIDAFVSSDPWFAGWCGVMVNVSDIAAMGGRPVAVVNALWGGN
                     DAATADVLRGMAAASRAYQVPIVGGHTNLRCAEPRLAVSILGRANRVLDAFAAKPGQV
                     LIAAIDLRGAYQPPYNHWNAATQAPPERLRGDIALLPAIAECGLATAAKDISQAGLLG
                     TCVMLLESSGTGVDIDLAAIPKPAGVDWVQWLTTFPSYGYLLATDARNVSALLARFHR
                     REIAAAAIGTLNASGLCQVHTDATSELFWDVQSTPLTALRKHHAGNLL"
     gene            complement(3448..4053)
                     /locus_tag="VFX11_10275"
     CDS             complement(3448..4053)
                     /locus_tag="VFX11_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEX4939052.1"
                     /translation="MTAAHFPEHYSDFTIKWATLDWEVQQALALRRAVFCQEQGLFDD
                     SDQDETDCSAQCIVAIANHGGWPDKVVGTVRIHQYSTGVWWGSRLAVSQEFRHRSGIG
                     AALIRLAVCSAHGLGGTQFFAQVQQQNEQLFQRLHWHSRYGLSVRNHPHVMMEADLSR
                     YPACHTPYTGYVLKGPLAATPDEIAPCLLHLPPESIHHVTH"
     gene            complement(4057..>5166)
                     /locus_tag="VFX11_10280"
     CDS             complement(4057..>5166)
                     /locus_tag="VFX11_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEX4939053.1"
                     /translation="QHLLTELQTHGIQLAQPEQLPLARKGGAGPSDHQAIVIDGQTLM
                     IPVHTHSAQRSPFSLDATSVTPRLTRNGIPVLDISFATRPRFYDLKTHDGIPYAHIAT
                     LHSTDVLATTVLQNCIRYDQPDTACQFCSIGQSLAAGNTIAHKTPQQLAEVAKAAVEL
                     DGVRHMVMTTGTPATHDRGATVLCDSVRAVKAAVNLPIQVQCEPPKDLHWLDELKKAG
                     ADALGLHLEVIHPDVRRRIMPSKSNITVDEYMRAFAHAVPLFGRGQVSTYILAGLGDT
                     QEEILALCTQLIALGVYPFVVPFVPIAGTALEHHPAPDSDFMNGILEPLAQMLADGDL
                     LSEKISAGCARCGACSSLKSYESRQRQTALPALEA"
BASE COUNT          940 a         1534 c         1679 g         1013 t
ORIGIN      
        1 cgaggaagtg gaagcagtgc tgggcagcgc cgaccagtgc gacgaaacct tcagcttccg
       61 tcagtgccag tggggtgatg ccagttccaa catccgcgtc aaatacctgg gcaacaaggt
      121 gatcggcctg tcgaagaaaa atctgcgctg aacggatagt agctggcctg ctagacttgg
      181 gggatgctcc tcaaggtgac agcgaggctg acatgagcag aaccatggat gcgcagcggg
      241 aacgcctgcc cgggttgatc cgcagcgact accgccagac ggcggcggat accgggctgg
      301 cccagccctc cgacgcggtg ttgcacgcgc tgcaggaagt gccgcgccac ctgttcgtgc
      361 agaaagcgtt tcaggattcc gcctattcca attcccccct gcccatcggc aatggccaga
      421 ccatctcgca acccttcatc gtggcgctga tgacggatct gctgttgctg aaaccgtctg
      481 acaaggtgct ggaaatcggc accggctgcg gttaccagag tgcgatcctg tcattgctgg
      541 cgaaggatgt ttattccctg gaaatcgtgt cggacctgta caagcagtcc actgaacggc
      601 tggcggggct gggctacgac aatgtgcatg tgcggctggg ggacggctac tacggctggc
      661 aggagcaggc gcccttcgat gcgattctgg tggcggccac ggcggacgct ctgccagagc
      721 cgttgctggt gcaactggcg ccgggcgggc gcatggtgat cccggtgcgg gatgccagcg
      781 ggcgcgaggt gctgcaactg gtgaccaagg atgccgctgg ccaggtgcag gtgagagaca
      841 ccatcggcgt gcggtttgtg ccgttgacgg gggagcactg agggctcccc gccgcagccg
      901 gtttcagccc agcgctgccg caaggcaggg attcattttc acctttggcg ggctggtgag
      961 tttgcctgcc tgagccagct gttgcgcaat gacctccacc acatacttcg catcctgatc
     1021 gatgcccgag aatcgtcccg agccccaggt gtacagccag ggcagaccga tgaaatacag
     1081 accgggaaac ggcgtgacgc cgcgctgatg ggccggatag ccgcgcccgt tgaagaccgg
     1141 aatgtccagc cagtgatagt ccggtgcgaa tccgatgcac cagatcacat ccgtgatatt
     1201 cgcgctgtcc agatccagag tttcccgttc ctgcggtggt tgccacacgg gttggtagcg
     1261 actggcgggt ggcgcgtcga tgctgtttgc cgcgatgaac tggtcgatgc gctcgttgat
     1321 gccattgtag gttttgtccg cactgtccag cacggcggcc aggttagggc tgaaacgcag
     1381 gtgtgttccg tccacatcga gaattttgcc gtacagctcc atgccttcca gcgcgaattt
     1441 gcgcagatcg atatcacgac cgccgtcgcg accggtcaca taatgattgg tattgtcgcg
     1501 cacgccttcc cggagcggat gttcgtgtac cggcatgtcg taatatttca tgtcggtgag
     1561 ccaggccacc acatcgcggc cacggtagaa gcgcgcgcag cggggcgcgt cgcccacggc
     1621 gagcatcacg cgtttgcctg ccaggtgcag atcctcggcg atctgcgcgc cggactggcc
     1681 ggagcccacc accagcactg cgccgtcgcc cagctggccg ggattcctgt actgttctga
     1741 atgcagttgc cggatggtgc ggggaagacg ctcggcgtag cggggaatga ccggggtctg
     1801 gtaactgccg gaggcgacca ccacctggtc cgcgctgaat gaaccctgtg tggtttcgat
     1861 ccggtagcgt tccccatcgt gtttgatgcg ggtgacagtc gtgtgctcca gtaccggcgc
     1921 gtccaccttc ctttcgaatg cgttcagata atcgatgatt tcatcgcgct tcatgaaacc
     1981 gtgcggatcg tcacccgtgt agggatgatc cggcaactgg cactgccagt tgggcgtgac
     2041 cagcgtgaag gaatcccagc gcttttcctt ccacgcatgc atgagccggt gcttctctgc
     2101 caccaggtgg ccgatattgt gctgctggca gtaatgactg atggagaggc cggcctggcc
     2161 gccgccgacg atgaaaaccg gatagtgctg catgatagtc tccagggttc tttttttcgt
     2221 gcagtcagga atgaatgcgc agcacggtga ccgcgccctg gccggaaaac tgctccgccg
     2281 tttgccggat gcgttgcagc tggtctgccg cgctggaaca gctgtagccg aatttctgtc
     2341 ttacgcgttc gctcgccagc atcagcgcgt cttcactgcg cgccagaaaa tccgccagtg
     2401 gataagtggt tgccggctgg aaaaaatccg ccaccacgct ggacggcgag tagcaggttt
     2461 cctcgtggcc atcgggccag cgcacatcaa agtaggtttc cggcatggtg tttcctcagg
     2521 gcagtgagcg gggtggattg gacgtcccag aacagctcgc tcgtggcgtc ggtgtgcacc
     2581 tggcacagac cgctggcgtt gagtgtgccg atggccgccg ctgcgatttc ccgccggtga
     2641 aaccgcgcca gcagtgcgga gacgttgcgg gcgtccgttg ccagcagata accgtagctg
     2701 ggaaaggtgg tgagccattg cacccagtcc acaccagccg gtttgggaat cgcggccaga
     2761 tcgatatcga caccggtgcc ggaggactcc agcagcatca cgcaggtgcc gagcaagccg
     2821 gcctggctga tgtccttggc ggcggtggcc aggccgcatt cggcaatggc tggcagcagc
     2881 gcgatgtcac cgcgcaggcg ttccggcggt gcctgggtgg cggcgttcca gtggttgtag
     2941 ggcggctgat aggcgccgcg cagatcaatg gcggcaatca gcacctgccc aggttttgct
     3001 gcaaacgcat ccagcaccct gttggcacgt ccaagaatcg ataccgccag ccttggctct
     3061 gcacagcgca gattggtatg gccgccaacg atgggcacct gataggcccg tgacgccgct
     3121 gccatgccgc gcagcacatc ggccgtcgcg gcgtcgttgc ctccccacag cgcgttcacc
     3181 accgcgacag ggcggcctcc catggcggca atgtcgctca cattcaccat cacgccgcac
     3241 cagccggcga accagggatc gctgctgacg aacgcgtcga taaaaccttc gccagccagc
     3301 agatcaaacc cgtcgccgct gggaatgacg gcggcgtcat cgccgttggg gaagggtgcc
     3361 ggatacgcct cggccaagtg ccgctgcacc agctggattt cggttttgca gtgcagaccg
     3421 gcgctggacc gcacggtgtc cagcaattca gtgagtgaca tggtgaatcg actccggcgg
     3481 caagtgcagc aggcagggcg caatctcgtc cggcgtggcg gcgagcggcc ctttcagcac
     3541 atagccggtg taaggtgtgt gacaggcagg ataacgggac agatccgctt ccatcatcac
     3601 gtgcggatga ttgcgcaccg acaaaccata gcgactgtgc cagtgcagac gctggaacag
     3661 ctgctcgttc tgctgttgca cctgggcaaa gaactgcgtg ccacccaggc cgtgggcgct
     3721 gcacaccgcc agccggatca gcgctgcacc gatgccggaa cgatgacgga attcctggct
     3781 caccgccagg cgcgatcccc accatacgcc ggtgctgtat tgatgaatgc gcacggtgcc
     3841 caccactttg tcgggccagc cgccgtgatt ggcgatggcg acgatacatt gtgcgctgca
     3901 gtcggtttcg tcctggtcac tgtcgtcgaa caatccctgc tcctgacaaa acaccgcgcg
     3961 tcgcagtgcc agtgcctgct gtacttccca gtccagggtg gcccatttga tggtgaaatc
     4021 ggaatagtgt tcgggaaaat gcgcagccgt catggttcag gcctccagtg ccggaagcgc
     4081 ggtctggcgc tggcgggatt cataactttt cagtgatgag caggccccgc agcgcgcgca
     4141 accggcactg attttttccg acagcagatc accgtccgcc agcatctgcg ccagcggttc
     4201 cagaatgcca ttcatgaaat cgctgtccgg agcaggatgg tgctccagtg ccgtgccggc
     4261 gatcggcacg aaaggcacca cgaagggata gacgcccagc gcgatcagtt gcgtgcacag
     4321 tgcgagaatt tcctcctgcg tgtcgcccag gcctgccagg atgtaggtgc tgacctggcc
     4381 gcgaccgaac agaggcacgg catgggcgaa agcccgcatg tattcgtcaa cggtgatatt
     4441 ggatttgctg ggcatgatgc gccgccgcac gtccggatgg atgacttcca gatgcaggcc
     4501 cagtgcatcg gcgccggctt ttttcagttc gtcgagccag tgcaggtcct tgggaggttc
     4561 acattgcacc tggatcggaa ggttcaccgc tgctttcact gcgcgtacgc tgtcgcacag
     4621 cacagtggcg ccgcggtcgt gggtggcggg tgtgccggtg gtcatcacca tgtggcgcac
     4681 gccatccagt tccaccgcgg cttttgccac ttccgccagt tgttgcggtg ttttgtgtgc
     4741 gatggtgttg cccgccgcca gcgactggcc gatagagcag aactggcatg cagtgtcggg
     4801 ctggtcgtag cggatgcaat tctgcagcac agtggtggcg agaacgtcgg tgctgtgcag
     4861 tgtggcaatg tgcgcatagg gaatgccgtc gtgcgttttc agatcataaa agcgtggccg
     4921 cgtggcaaag ctgatatcca gcacgggtat gccgttgcgc gtcagccggg gcgtgaccga
     4981 cgtggcgtcc agactgaacg gcgaacgctg tgcgctgtgg gtgtgcaccg gaatcatcag
     5041 cgtctggccg tcgatcacaa tggcctgatg atcgctggga ccggcgccac ccttgcgggc
     5101 aagtggcagt tgctccggtt gcgccagctg gatgccgtgg gtctgcaatt cagtcagcag
     5161 atgctg
//
LOCUS       DASUEG010000545         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_841791, whole genome shotgun sequence.
ACCESSION   DASUEG010000545 DASUEG010000000
VERSION     DASUEG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841791"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFX11_10285"
     CDS             <1..670
                     /locus_tag="VFX11_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX4939054.1"
                     /translation="QCLNATMTYRHQHGSTKGVEQWISELGWRDFYKHVMVGWPHVCK
                     HRPFRLETSHIPWRHDEGDFQRWCEGRTGFPLVDAAMRQLRQTGWMHNRLRMVVAMFL
                     TKDLFIDWRWGERFFMQHLIDGDLSANNGGWQWSASTGNDAAPYFRIFNPVLQSERFD
                     PKGNFIREYVPELRHIHDKSIHQPGASLFSEYPAPMVDRRIVKNRVLSHFRDLKPLTQ
                     AAAR"
     gene            complement(718..1506)
                     /locus_tag="VFX11_10290"
     CDS             complement(718..1506)
                     /locus_tag="VFX11_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4939055.1"
                     /translation="MRRVIFNQKGGVGKSSITVNLAAISASEGLKTLVVDLDPQCNAT
                     QYLLGEVAEFSRDKPELSPNIGQFFAQTLQFKLKEKPLSEYVHRTPFENLYVIPSNPE
                     LGEIEHLLESKHKIFKLASALRELSSTYDAIYIDTPPAFNFYTLSALIAAQRCLIPFD
                     CDAFSRRALYTLLENVGEARADHNDELEVEGIVVNQFQPRASLPVKLVEELRAEGLPI
                     VSSHLSSSVIMKESHEKALPLVHLSPKHKLTEEYVALFRELNPA"
     gene            complement(1599..2828)
                     /locus_tag="VFX11_10295"
     CDS             complement(1599..2828)
                     /locus_tag="VFX11_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX4939056.1"
                     /translation="MLNRIWLGFFLIAFVACLYQATLGGQPEVVAGAVDASFKAAKTS
                     VEIALGLTGLLCFWLGLFQIAEKAGLIEKMAWMLAPLFRRLMPEVPAGHPATGSVTMN
                     LAANMLGLDNAATPLGLKAMKDLQTLNPVPDTATNAQILFLVLNTASVTLIPVTVFLY
                     RAQFGATNPASVFVPILITTTVGTLAGLLMVAWIQKLKLLDRVVMAYAAGLAALMGLV
                     VAAVITAPSEMLGVRSAFVGNLLLLSIIVVFLLAGWRKGIDVYDAFITGAKQGFEVAI
                     GLIPFLVAMLVAVAVFRASGAMDVLLSGVATVFAWLGANTDFVPALPTAIMKSLSGSG
                     ARALMIETIQTYGVDSFPARVAAIIQGSSETTLYVLAVYFGSVGIRKERHALACGLFA
                     DLVAVVVAILVAYWFFH"
BASE COUNT          704 a          946 c          788 g          558 t
ORIGIN      
        1 acaatgcctg aacgccacca tgacgtatcg gcatcagcac ggcagtacca agggtgtcga
       61 acagtggatc agcgaactcg gctggcgtga cttctacaag cacgtaatgg tgggttggcc
      121 tcatgtctgc aagcaccgcc ccttccggct ggagacctcg cacatccctt ggcgacatga
      181 cgagggggat tttcaacgct ggtgcgaagg ccgtaccggc tttcctttgg tcgatgccgc
      241 catgcggcag ctacgacaga ccgggtggat gcacaatcgt ctgcgcatgg tcgtcgccat
      301 gttcctgacc aaggacctgt tcattgactg gcgctggggc gaacgcttct tcatgcagca
      361 cctgattgac ggagacctgt cggccaacaa cggcggctgg caatggtctg cctcgaccgg
      421 aaacgatgcg gccccctact tccgcatctt caaccctgtc cttcaaagcg aacggtttga
      481 ccccaagggc aacttcatcc gggagtatgt tccggagctt cgccacatcc atgacaagtc
      541 cattcatcag cctggcgcat ctctattcag tgaataccct gcccccatgg tggaccgccg
      601 catcgtcaag aaccgagtac tgtctcactt tcgcgacctc aaaccgctaa cacaggccgc
      661 cgcacgctaa ataaaaaacc gccctgcagg cggtttttta tcatccaaag agacccatta
      721 cgccggattc agctcccgga acagggcaac gtactcttcg gtcagcttgt gtttgggcga
      781 gaggtgaacc agcggcaacg ccttctcgtg agactccttc atgatcacgg aggacgacag
      841 gtgagagctt acaatcggca agccctcagc gcgcagctcc tcaaccagct tcaccggcag
      901 actggctcgc ggctggaact gattgacgac aatcccttca acctccagct catcgttatg
      961 gtcggcacgc gcctcgccca cattctccag cagggtatac agggcacggc gtgagaacgc
     1021 atcgcaatca aacggaatca ggcagcgctg cgccgcaatc agggccgaca gggtatagaa
     1081 gttgaacgcc ggcggggtgt cgatgtagat cgcatcgtag gtgctgctaa gctcacgcag
     1141 agccgaggcc agcttgaaga ttttgtgctt gctctccagc agatgctcaa tttcccccaa
     1201 ctcgggattc gagggaatca cgtacagatt ctcaaacggc gtacggtgca catactcgga
     1261 cagcggcttt tccttgagct tgaactgcag cgtctgggca aagaactggc cgatgttagg
     1321 gctcagctcg ggcttgtcgc gagagaactc agccacctca cccagcaggt actgcgtcgc
     1381 attgcactgc ggatccaggt ccacgaccag ggtcttgagc ccctcactgg cactgatggc
     1441 ggccagattc accgtgatgc tggatttccc caccccgccc ttctgattga agatgacacg
     1501 acgcatcccg aaactcccct gtcaaagcca ctgccgatag gttgcggatt gtaacggcaa
     1561 cccttggccc cgtgaacgat tattgaccgt ccataagatc aatggaaaaa ccagtaagcc
     1621 accaggatgg cgaccaccac cgccaccaga tcggcaaaca gaccacatgc cagcgcatgc
     1681 cgctccttgc gaataccgac cgacccgaaa tacaccgcca gcacatagag ggtcgtttct
     1741 gacgaccctt gaatgatggc cgccacacgc gccggaaatg aatccactcc gtaggtctgg
     1801 atggtttcaa tcatcagggc tcgtgcacct gaaccggaaa gcgacttcat gatggccgtg
     1861 ggcagcgccg gtacaaaatc ggtattggcg cccagccagg caaagacagt ggccacaccg
     1921 gacagcaata catccatggc accgctggcc ctgaacaccg ctacggccac cagcatggcc
     1981 accaggaacg ggatgaggcc gatggccacc tcaaaaccct gtttggcacc cgtgatgaac
     2041 gcatcataga catcaatgcc tttccgccac cccgccagca gaaacaccac aatgatggac
     2101 aacagcagca ggttgcccac aaacgccgac cgcaccccga gcatttccga gggcgcggta
     2161 atgaccgccg ccacgaccag ccccatcagc gccgccagtc cggccgcata tgccatgacc
     2221 acccgatcca gcagcttcaa cttctgtatc catgccacca tgagaaggcc cgccagagta
     2281 cctaccgtcg tggtaatgag gatggggaca aatacactgg ccggattggt cgcaccaaac
     2341 tgggcacggt aaagaaagac tgtcacaggg atcagggtga cagaggccgt gttcaacacc
     2401 agaaagagta tctgggcatt ggtcgctgta tctggcaccg ggttgagtgt ctgcagatcc
     2461 ttcatcgcct ttagacccag cggggtcgcg gcgttatcca ggcccagcat gttggcggcc
     2521 agattcatgg tcaccgaccc cgtcgctggg tgcccggcag gcacctccgg catcaggcga
     2581 cgaaacaggg gcgccagcat ccaggccatc ttctcgatca gacctgcctt ctcggctatc
     2641 tggaacagcc ccagccagaa gcacaacaag cccgtcagtc ccagggcaat ctccactgac
     2701 gtcttggcgg ccttgaagct ggcatccaca gcccctgcga ccacctccgg ctggccaccc
     2761 aacgtcgcct gatacaggca cgccacaaag gcaatcagaa agaaccccag ccagatacgg
     2821 ttcaacatta gcgatccttg cgacacgcca gaaaaaacgt gggtatgagc cccgaagcag
     2881 caagtgaaac ggaatcatct cgatggctca agctctgccg tatccgagcg cctgtttttt
     2941 gtacaacctc atgttttttc gagaaaacag ctatccacat tttctgtgga taagtt
//
LOCUS       DASUEG010000546         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_206022, whole genome shotgun sequence.
ACCESSION   DASUEG010000546 DASUEG010000000
VERSION     DASUEG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206022"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VFX11_10300"
     CDS             <1..1467
                     /locus_tag="VFX11_10300"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HEX4939057.1"
                     /translation="TRAQSRRVPVTSNPAPNDATGAAFVKAGENFDVLATAVQYESAD
                     DSNADGVPDAGADLSGNGTAPAFGQEPAGVRDTVALSSTLVLPSAAGSVHPGLSGATS
                     ITSFTSGAGSAITSYGEVGIISISAALADNSYLGSSNVTGNIPRVGRFYPDHFTAAHN
                     SPTLRDGSGSWSCDFTYQGQPFGFATEPQVTFTAVNASGNPTLNYTGDFWKLDVPQHG
                     IALDASSPPAGSACISSGAVVAGCFSENSASVARSWSGTDSYDGQGVFFTGSHVLQIN
                     KLNAMPNAGDVPYPPRVDLVLPAAEITDNDGACYLSGGSCSEFRIDDISGTDIRYGRG
                     WIDNTNGPVMTPLIMTLRLQYWDASANFVYNTLDNAGCSGTPALTTDIQLSNFTSNLQ
                     AGETSVAALSAQPGYYLASLAAPGSDSSGQPNAGTAIVTWLLDNDTSDNAPGEACSQH
                     WLCYDYDGNGLNENPAARAIFSTLPDKRPLLFLKEAYR"
     gene            1552..1803
                     /gene="hypC"
                     /locus_tag="VFX11_10305"
     CDS             1552..1803
                     /gene="hypC"
                     /locus_tag="VFX11_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX4939058.1"
                     /translation="MCLGVPGQIESIADPELALAWVRLEGVRREVNVACVLDGESIDG
                     LVGSWVIVHAGFALEKINLEEAQCTLQLLQQLHSAQETH"
     gene            1803..2906
                     /gene="hypD"
                     /locus_tag="VFX11_10310"
     CDS             1803..2906
                     /gene="hypD"
                     /locus_tag="VFX11_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX4939059.1"
                     /translation="MELLQTFRDPAHVRTLAALVREAGATLAQQWQRPVQVMEVCGGH
                     THAIYKAGLHQLVGNQIEFLHGPGCPVCVLPKSRIDACIAMAQQADVILATYGDVMRV
                     PGTALSFIQAKALGADIRMVYSALDALRLAQRNPHKRVVFFALGFETTMPATALTLQQ
                     ARHQQVQNFFVYCHHILIVPALTALLKDQDTLIDGFIGPGHVSAVLGTAAYDFIAAEF
                     RKPFVIAGFEPVDILQSLLMVLRQLQQGRCAVEVQYNRVVTAAGNPVAQAALADVFRV
                     APHSEWRGLGVLEQSGLMLQPAYAQHDAALHFQLEERPEITAEAGRCGAVLKGQIKPA
                     QCELFGNQCTPQHPVGALMVSSEGACSACYQHA"
     gene            2899..3228
                     /locus_tag="VFX11_10315"
     CDS             2899..3228
                     /locus_tag="VFX11_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX4939060.1"
                     /translation="MHELSLVEDILRIAGKQAGGRTVKRVVLEVGALTCVMPEALEFC
                     FDSCKAAAALEQAQLVIERRAGVARCEQCGSEFELDELYQPCACGSFDKAILQGQELR
                     ILSLELE"
     gene            3228..4028
                     /gene="hypB"
                     /locus_tag="VFX11_10320"
     CDS             3228..4028
                     /gene="hypB"
                     /locus_tag="VFX11_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX4939061.1"
                     /translation="MCTTCGCDASSSSIYRPEAPAASALQSAVFAAVPAPRAPAEPGG
                     QTTHQHLEIALLQRNDDIAAGNRDTFARQGTLALNLVSSPGAGKTTLLEKTLHAIARA
                     VPVAVIEGDQATDQDAQRIAATGVKVVQVNTGTGCHLDADMIRRAALQLQPLQQHLLL
                     IENVGNLVCPALFDLGEKMKIVLLSVTEGDDKPLKYPHMFQAAGLLLITKCDLLPHVD
                     FDVDACIARARQINPRLDVLQLSSRTGQGMAVWQQWLLDRLSAVAKTG"
BASE COUNT          785 a         1248 c         1265 g          776 t
ORIGIN      
        1 acgcgagcgc aaagccggcg cgtgccggtt accagtaacc ccgcccccaa cgatgcaacc
       61 ggtgcagcct ttgtgaaagc gggagaaaat ttcgatgtgc tcgcaacagc agtgcaatat
      121 gaatccgccg atgacagcaa cgccgacggc gtgcctgatg cgggcgcaga tctgagtggc
      181 aacggaactg caccggcttt tggccaggaa cccgccggtg tgcgcgatac ggttgcgctg
      241 tccagtaccc tggtgctgcc cagtgcggca ggcagcgtgc atccaggatt atccggcgcc
      301 accagcatca cctccttcac cagtggagcc ggcagcgcca tcacgtccta tggcgaagtc
      361 ggcatcattt ccatcagtgc agcactggcg gacaacagct atctcggcag cagcaatgtc
      421 accggcaaca ttccgcgggt cggccgtttt tatccggacc atttcaccgc cgcccacaat
      481 tcaccaacct tgcgtgatgg cagcggcagc tggagctgcg atttcaccta tcagggacag
      541 cccttcggtt ttgcaacgga accgcaggtg acattcactg ccgtcaacgc cagcggtaac
      601 cccacgctga attacaccgg tgatttctgg aagctggacg tgccgcagca cggcattgca
      661 ctggacgctt ccagcccgcc tgccggctcc gcgtgcatca gcagcggcgc agtggttgcc
      721 ggttgcttta gcgaaaattc cgccagcgta gcgcgcagct ggagtggcac cgacagctat
      781 gacggccagg gtgtgttctt caccggcagc catgtcctgc agatcaacaa gctcaacgcg
      841 atgccgaatg ccggcgacgt tccttacccg ccgcgggtgg atctggtgtt gccggccgca
      901 gagatcacag acaatgacgg tgcctgctac ctgagtggtg gcagctgcag tgaattccgt
      961 attgatgaca tcagtggcac cgacatccgc tacggccgcg gctggatcga caacaccaac
     1021 ggcccggtaa tgacgccgct gatcatgacg ctgcggctgc aatactggga tgccagcgcg
     1081 aacttcgtct acaacacgct ggacaatgcc ggctgctcgg gcaccccggc gctgacgacc
     1141 gacatccagc tgtcaaattt caccagcaac ctgcaggcgg gcgagacgtc ggtggcggca
     1201 ctgtctgctc aacccgggta ttaccttgcc agtctggcgg cccccggttc cgacagcagt
     1261 ggccagccca atgccgggac ggccattgtc acctggctgc tggacaacga caccagtgac
     1321 aacgctccgg gtgaagcctg cagccagcac tggctgtgct acgactacga cggtaacggg
     1381 ctgaatgaaa atcccgccgc ccgcgcaatt ttcagcacat tgccggacaa gcgccccctg
     1441 ctgtttctga aagaggctta tcgctgatcc ggtccagccc ttgctcctga ccattccgct
     1501 tgaccctttt cccaggacgc gccacgctaa cagcctataa caggaggctc tatgtgtctt
     1561 ggcgttcccg gtcagattga atccattgcc gatcccgaac tcgcgctggc gtgggtgcgg
     1621 ctggaaggcg tgcgccgcga agtcaacgtg gcctgcgtgc tggatggcga gtccatcgac
     1681 gggctggtgg gcagctgggt catcgtgcac gcggggttcg cgctggagaa aatcaatctg
     1741 gaagaagcgc agtgcacgct gcagctgttg cagcaattgc attccgcaca ggagacccat
     1801 tgatggaact gctgcaaacc tttcgcgatc cggcccatgt gcgtactttg gcagcgctgg
     1861 tgcgggaagc gggtgcaacg ctggcgcagc agtggcaacg gccggtgcag gtcatggaag
     1921 tgtgcggtgg ccatacccac gccatctaca aggccggcct gcatcaactg gtgggcaatc
     1981 agatcgagtt tctgcacggc cccggttgtc cggtttgcgt actgcccaaa tcccgcatcg
     2041 atgcctgcat cgcgatggcg caacaggcgg atgtgattct ggcaacctac ggcgatgtca
     2101 tgcgtgtgcc gggtacggcg ctgagtttta tccaggccaa ggcactgggt gccgacatcc
     2161 gcatggtgta ttccgcgctg gacgcgttgc gactggcgca gcgcaatcca cacaaacgcg
     2221 tggtgttttt cgcgctgggt tttgaaacca ccatgccggc cacggcgctg acgctgcaac
     2281 aggccaggca ccagcaggtg cagaattttt tcgtctactg ccaccatatt ctgatcgtac
     2341 ccgcactgac ggcgttgctg aaagatcagg acacgctgat tgacggcttc attggtcccg
     2401 gtcatgtcag cgcggtgctg ggtacggccg cctacgattt cattgcggca gaatttcgca
     2461 agcccttcgt gatcgccggc ttcgaaccgg tggatatcct gcagtcgctg ctgatggtgt
     2521 tgcggcaatt gcagcaaggc cgctgtgcag tggaagtgca gtacaaccgt gtcgtaaccg
     2581 ctgccggcaa tccggtggcg caggctgcac tggcggacgt gtttcgagtg gcgccgcaca
     2641 gcgaatggcg tggcctgggt gtactggagc aatccggcct gatgttgcag ccggcttacg
     2701 cgcagcatga tgccgctctg cactttcagc tggaagagcg cccggaaatt acggcagagg
     2761 ccggccgctg cggcgcggtg ctgaaaggcc agatcaagcc ggcccagtgc gaactgttcg
     2821 gcaaccagtg cacgccgcag catcccgtcg gcgcgttgat ggtgtcgtcg gagggcgcct
     2881 gttcggcctg ttaccagcat gcatgaactg agtctggtgg aagacattct gcgcatcgcc
     2941 ggcaaacagg ccggtggccg caccgtgaag cgtgtggtgc tggaagtggg cgctctcacc
     3001 tgtgtgatgc ccgaagcact ggaattctgt ttcgacagct gcaaggccgc cgccgcgctg
     3061 gaacaggcgc agctggttat cgagcggcgc gccggtgtcg cccgctgcga gcaatgcggc
     3121 agtgaatttg aactcgacga actgtatcag ccctgcgcct gcggcagctt cgacaaggcc
     3181 atcctgcagg ggcaggaatt gcggattctt tccctggagc ttgaataatg tgtaccacct
     3241 gcggttgcga cgcatcctcc agcagcattt accggcccga agcgcctgcg gcgtcagcat
     3301 tgcagtcggc ggtattcgct gctgtaccgg caccgcgcgc gccggctgaa ccgggcgggc
     3361 agacgaccca tcaacatctc gaaatcgcgc tgctgcagcg caacgatgac atcgctgccg
     3421 gcaaccggga cacattcgca cgtcagggca cactggcact gaatctggtg agttccccgg
     3481 gcgccggcaa aaccacgctg ctggaaaaaa ccctgcacgc catcgcacgc gcggtgccgg
     3541 ttgccgtgat cgaaggcgat caggccaccg atcaggacgc gcaacgcatc gccgccactg
     3601 gcgtgaaagt ggtgcaggtg aataccggca ccggctgcca tctggatgcc gatatgatcc
     3661 gccgcgccgc gctgcagctg cagccgttgc aacagcacct gttgctgatc gagaacgtcg
     3721 gcaatctggt atgcccggcg ctgttcgatc tgggagagaa aatgaaaatc gtgctgctgt
     3781 ctgtcaccga aggggacgac aagccgttga agtatccaca catgttccag gcagcgggcc
     3841 tgctgctgat caccaagtgc gatctgttgc cccatgtgga tttcgatgtg gacgcctgca
     3901 tcgcccgcgc ccgtcagatc aatccacggc tggacgtgct gcagctttca tcccgcacgg
     3961 ggcagggcat ggcggtctgg caacagtggc tgctggaccg gctgtcggct gtggcaaaaa
     4021 ccggttgacg attcggtccg gcgtgctaga tttcggggct agaactgtca acga
//
LOCUS       DASUEG010000547         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_201235, whole genome shotgun sequence.
ACCESSION   DASUEG010000547 DASUEG010000000
VERSION     DASUEG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201235"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="thrC"
                     /locus_tag="VFX11_10325"
     CDS             <1..807
                     /gene="thrC"
                     /locus_tag="VFX11_10325"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4939062.1"
                     /translation="SAAAAAYAARAGITAFVLIPEGKIAMGKLAQAMMYGAVTLQIRG
                     NFDEGMQLVKEVAAEAPVTIVNSINPFRLQGQKTAAFEIVEDLGAAPDYHCLPVGNAG
                     NITAHWMGYSEYHKAGLANSRPKMIGYQAAGSAPFMRGHMVDQPETIATAIRIGHPQS
                     WDKAWVVQKESGGWFDELTDAEILQTQRLLAQHEGVFCEPASAASLGGAIRDVKNGKI
                     PEGSKIVCTLTGNGLKDPDTAIAQCQNAGMFTIDAKLGAVKDAILKNMAG"
     gene            968..>2127
                     /locus_tag="VFX11_10330"
     CDS             968..>2127
                     /locus_tag="VFX11_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX4939063.1"
                     /translation="MKKITRRTPPASHQFSGAVHPALQRIYASRGVLNDEQIDRSLKH
                     LFPAQLLKGLPQALDLLVDALQQQKKILIVGDFDADGATSSALSVLALRALGAHHVDY
                     LVPNRFKYGYGLTPEIVEVAKQRAPDLLITVDNGISSIEGVAVAREAGMNVLVTDHHL
                     PSQQLPDANAIVNPNQPGCDFPSKNLAGVGVIFYVMTALRTKLKEIGWFEQQKIPEPN
                     MADYLDIVALGTVADVVPLDQNNRILVHNGLMRMRAGRCRPGIKALVDVAQRKLERLV
                     AADLGYMIGPRLNAAGRLDDMSHGIECLLTDNPGLAKDFAQELDQLNHERKSIESGMQ
                     QQALAALNMIHLKPDQQMPWGLCIYKKDWHQGVIGILASRIKERFHRPVIAFA"
BASE COUNT          444 a          605 c          676 g          402 t
ORIGIN      
        1 tccgctgccg ctgccgctta tgctgcgcgt gccggcatca ccgcattcgt gctgattccc
       61 gaaggcaaga ttgcgatggg caagctggcg caggcgatga tgtacggtgc cgtgacgctg
      121 cagattcgtg gcaacttcga cgaaggcatg cagctggtga aagaagttgc ggcggaagca
      181 ccggtgacca tcgtgaactc gatcaacccg ttccgtctgc agggccagaa aaccgctgcg
      241 ttcgaaatcg tggaagacct gggcgccgcg ccggattacc actgcctgcc ggtggggaac
      301 gcgggcaaca tcaccgcgca ctggatgggt tactccgaat accacaaggc cggcctggcc
      361 aacagccgtc cgaaaatgat cggctaccag gccgccggct ctgcgccgtt catgcgcggc
      421 cacatggtgg atcagccgga aaccatcgcc actgcgatcc gtatcggcca cccgcaaagc
      481 tgggacaagg cgtgggtagt gcagaaggaa tccggcggct ggttcgacga actgaccgac
      541 gccgaaatcc tgcagacaca acgcctgctg gcgcagcacg aaggtgtgtt ctgtgaaccg
      601 gcatcggccg cttccctggg tggtgcgatc cgtgacgtga aaaatggcaa gattccggaa
      661 ggcagcaaga tcgtctgcac actgaccggc aacggcctga aagatcctga taccgcgatc
      721 gcccagtgcc agaacgccgg catgttcacc atcgacgcca aactgggcgc cgtgaaggac
      781 gccattctga aaaacatggc aggctgatcc ggccaggctg aatggcaggg atgccgtcag
      841 gtcaacgagg cgtacaacaa aaagggagtc gcgcaggtcg gctccctttt gtttttcggt
      901 ccggatagtt ttgcgctatc ctgtttttca attcactctc tgatcgttcc gcgcagacgg
      961 ggaccgcatg aaaaaaatca cccgccgcac ccctcccgct tcgcaccagt tttccggtgc
     1021 cgtgcacccg gcgctgcagc gtatttacgc cagtcgcggt gtgctgaatg acgagcagat
     1081 cgatcgcagc ctgaaacatc tttttcccgc ccagttgctg aaagggctgc cgcaggcgct
     1141 cgacctgctg gtggacgcgt tgcagcagca gaaaaaaatc ctgatcgtgg gtgatttcga
     1201 tgccgacggt gccaccagca gcgcgctgtc ggtgctggcg ttgcgtgcgc tgggtgcgca
     1261 ccatgtggat tatctggtgc cgaaccggtt caagtacggt tatggactga cgccggaaat
     1321 tgtcgaggtg gcaaaacagc gcgcaccgga tctgctgatc acggtggaca acggtatttc
     1381 cagtattgaa ggcgtggcgg tggcacggga agcgggcatg aacgtgctgg tgaccgacca
     1441 ccatctgccg tcgcagcagt tgccggacgc caacgccatt gtgaatccca accagcccgg
     1501 ctgcgatttc ccgtcgaaaa acctggcggg cgtcggcgtg attttttatg tgatgacggc
     1561 gctgcgcacc aaactgaagg aaatcggctg gttcgagcag cagaaaattc ccgagcccaa
     1621 catggcggat tatctcgata tcgtggcgct gggcacggtg gcggatgtgg tgccgctgga
     1681 ccagaacaac cgcattctgg tgcacaacgg tttgatgcgg atgcgggcgg ggcgctgccg
     1741 gccgggcatt aaagcgctgg tggatgtggc gcagcgcaag ctggaacggc tggtggcggc
     1801 ggacctgggt tacatgattg gcccgcgtct gaatgcagcg gggcggctgg acgatatgtc
     1861 gcatggcatt gaatgcctgc tcaccgacaa tcccggtctg gcaaaggatt ttgcgcagga
     1921 actggaccag ctcaatcacg agcgcaaatc cattgaaagc ggcatgcagc agcaggcgtt
     1981 ggccgcgctc aacatgattc acctgaagcc ggaccagcag atgccctggg gtctgtgcat
     2041 ctataaaaag gactggcacc agggcgtgat cggcattctg gcgtcgcgta tcaaggaacg
     2101 gtttcatcgc ccggtgattg cgtttgc
//
LOCUS       DASUEG010000548         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2154598, whole genome shotgun sequence.
ACCESSION   DASUEG010000548 DASUEG010000000
VERSION     DASUEG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154598"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFX11_10335"
     CDS             complement(<1..481)
                     /locus_tag="VFX11_10335"
                     /inference="COORDINATES: protein motif:HMM:NF037677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4939064.1"
                     /translation="MHIIRARDAEQKQQRRQTLIDAGWAIYLESDGQLPSVAQISARA
                     GLAKGTFYIYFKTKEELFVELVAAAMDELFLKLNTYLDQPDSTLHGCIEVFINAVGRE
                     DPIIKLGPMLTGVLERNTEPAIIIGFKRRLVANLLHCGQNLARRFPGLDAHTAARTLM
                     "
     gene            644..>3371
                     /locus_tag="VFX11_10340"
     CDS             644..>3371
                     /locus_tag="VFX11_10340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939065.1"
                     /translation="MARIKWWGWTLLVAALYFGFAGIGQLLAVPGSDVAPVWPPSGIA
                     LALVLLFGKRLLPGVFLGAFVTQFHQSYWINGGADLAWALGASFGVALGNTSETLLAG
                     WLIQRFAGRHPFESLRGVLVFTGAAAAGCALAALVATLTLAENGQGMAFMFRFLLTWW
                     LGDLAAILVLTSLLLGWSELMREHRSTRQLVHLFIFLALFMLGLNLSFAPPAQLGFPA
                     PMLFLMLPVLVWGAVAFRPPEMSLILFLFAGFTLYRTLHGGGMFAGADLHQSLLQLQL
                     MVCVVGVSQYVINGAVLERHRLNRRLMRINQDLEQTVRERVQALEQANRHLQERVQQQ
                     ARSEKRLQDSEDRLSLITQATTDGIWEWESARRQFRYSSRFKALLGYDQFDQLPLSLR
                     FWLRRILAEDRAGLLRACRDHLQRRAPLNASFRMRNKSGQLHWYRVQGQATWNARGEP
                     IQLAGSISDVDAEKSAHEILVSEKRLLERMTSGARVNEVLQLAADLLCVRFRHAGCSI
                     VLLDESCVNTVQVAQVEVSPELQEFMAGVKVGPRAVGASAAIYFNTQLVVDDIQQHPD
                     WRPYLGLLRHSGLRACWAHPIRNRHGSVLGAIALHFSACCYPSVEESEFLDRVARLAG
                     IAVEQDATRSVLQTSEQRYRELYHNNPAMFFSLDRDGVIRSVNQFGAEHLGYVPADLV
                     DRDYSELIEESRRDELREALRQAIETPGRVQVFEARKQCADGRWLWVRDSLRSMGSQQ
                     GRTEVLVVAEDITDIRELSDRLAFQAAHDSLTGLINRRKFEAELVNAIYEAGALNMSH
                     VLCYLDLDLFKVINDTCGHAAGDELLRQLGRLLEETVDGKGLIARLGGDEFGLLIRNC
                     ELDDAVQLAGVVRDKIAEHQFVWDRRPYHVGVSIGLVRIGQETTHLTALMSAA"
BASE COUNT          556 a          946 c         1164 g          705 t
ORIGIN      
        1 gcatgagggt gcgggcggca gtgtgggcgt ccaggcccgg gaagcgccgc gccaggttct
       61 ggccgcagtg gagcaggttg gccaccagac ggcgcttgaa gccgatgatg atggcgggtt
      121 cggtattgcg ctccagcaca ccggtcagca tgggaccgag cttgatgatg gggtcttcgc
      181 gaccgacggc gttgatgaag acttcgatgc agccgtggag cgtcgaatcg ggctggtcca
      241 gataggtgtt gagcttgaga aagagttcgt ccatcgcggc cgccaccagt tcgacaaaca
      301 gttcttcctt ggttttgaag tagatataga acgttccctt ggccagaccg gcgcgggccg
      361 agatctgcgc caccgacggc agctggccgt cgctttccag atagattgcc cagccggcat
      421 cgatcagggt ttgccggcgc tgttgttttt gttctgcatc gcgggcgcgg atgatatgca
      481 tgcggattcc cctgcaaaaa aaccgattat atcgtgaact ggcttgattg acggcagctt
      541 tcgcggcggc aaccgtcggg cggtcaacta tgctttctct tggggtcgtg aagacttcgt
      601 taatgatgcg atcgctttcg atggattgaa caggagccag tcaatggccc ggatcaaatg
      661 gtggggctgg acgctgctgg tggcggccct gtacttcggc tttgccggca ttggccagtt
      721 gctggcggtg ccggggagcg atgtcgcgcc ggtgtggccg ccgtccggca tcgcactggc
      781 gctggtgctg ctgtttggca agcgcctgct cccgggggtc ttcctggggg cctttgtcac
      841 ccaattccac cagtcctact ggatcaatgg aggcgcggac cttgcctggg ccctgggtgc
      901 ttccttcggt gtggcgttgg gcaataccag cgagaccctg ctggcgggct ggctgatcca
      961 gcgctttgcc ggccgtcacc cttttgagtc actgcggggg gttctggttt ttaccggcgc
     1021 cgctgcagcc ggttgcgcgc tggcggctct ggtcgccacc cttaccctgg cagagaacgg
     1081 gcaggggatg gccttcatgt tcaggttcct gctgacctgg tggctggggg atctggcagc
     1141 gatactggta ttgacctcgt tgttgctggg ctggtcggag ctgatgcgcg agcaccgcag
     1201 tacccggcaa ctggttcacc tgttcatttt tctcgccctg ttcatgctgg ggctgaatct
     1261 gtccttcgcg ccgccggcgc aactgggttt tcccgcgccg atgctgttcc tgatgctgcc
     1321 ggtgctggtg tggggtgcgg tggcattcag gccgccggaa atgagcctga tcctgtttct
     1381 gtttgccggc ttcaccctgt atcgcacgct gcatggcggg ggtatgtttg ccggcgcgga
     1441 tctgcaccag tcgctgctgc aactgcagct gatggtgtgc gtggtgggcg tgagccagta
     1501 cgtaataaac ggcgcggtgc tggagcgtca ccggttgaac cgccggctga tgcgcatcaa
     1561 tcaggacctg gagcagacgg tgcgggagcg ggtgcaggca ctggaacagg ccaaccgcca
     1621 tctgcaggag cgggtacagc agcaggcgcg cagcgaaaag cgcttgcagg acagcgagga
     1681 ccggttgtcg ctgatcacgc aggcgaccac cgatggtatc tgggaatggg agtcggcgcg
     1741 gcgccagttc cgctattcca gccgcttcaa agcgctgctg ggctacgacc agttcgacca
     1801 gttgccgctc tcgctgcgct tctggctgcg ccgcattctg gcggaagacc gtgccggcct
     1861 gttgcgggcc tgccgcgatc acctgcagcg gcgcgcgcca ctgaacgcca gtttccgcat
     1921 gcgcaacaaa tccggccagc tgcactggta ccgggtgcag ggtcaggcca cctggaacgc
     1981 acgcggtgaa ccgatccagc tcgcgggctc catttccgat gtggatgccg agaaatccgc
     2041 ccacgaaatc ctggtgagtg agaagcgcct gctggaacgc atgaccagcg gcgcccgcgt
     2101 gaatgaagta ctgcaattgg cggcagatct gctgtgcgtc cgtttccggc atgcgggctg
     2161 ttccatcgtg ttgctggatg agagctgcgt gaatacggtg caggtggcgc aggtggaggt
     2221 atcgccggag ctgcaggagt tcatggcggg cgtgaaagtg gggcctcgcg cagtgggtgc
     2281 cagcgcggca atctatttca atacacagct ggtggtggat gatatccagc agcacccgga
     2341 ctggcgaccc tacctgggtc tgctgcgcca cagcggattg cgcgcctgct gggcccatcc
     2401 gatccgcaat cgtcatggca gtgtgctggg tgccattgcg ctgcatttct ccgcttgctg
     2461 ctacccgtcg gtggaagaat cggaatttct tgaccgggtg gcgcggctgg cgggcattgc
     2521 ggtggaacag gacgccaccc gcagcgtcct gcagaccagc gagcagcgat accgcgagct
     2581 ctaccacaat aatcccgcca tgtttttttc cctggatcgt gatggcgtga tccgttcggt
     2641 gaaccagttt ggcgctgaac acctgggcta tgtaccggcg gatctggtgg accgtgacta
     2701 cagcgaactg atcgaagagt cgcgccgcga cgaattgcgc gaagccctgc gccaggcgat
     2761 cgaaacgccg ggtcgggtgc aggtgttcga ggcacgcaag cagtgtgccg atggccgctg
     2821 gctgtgggtg cgggattcgc tgcggtcgat gggatcgcag caggggcgca ccgaggtgct
     2881 ggtggtggcg gaagacatca ccgacattcg cgagctgtcg gaccggttgg ccttccaggc
     2941 cgcgcacgat tccctcactg gcctgatcaa ccggcgcaag ttcgaggcgg agctggtgaa
     3001 tgcgatctat gaggcgggcg cgctcaatat gtcccacgtg ctgtgctatc tggatctgga
     3061 tctgttcaaa gtgatcaatg acacctgcgg ccacgcggcg ggcgacgaac tgttgcggca
     3121 actgggccgg ctgctggagg aaacggtgga cggcaagggc ctgatcgcac gtcttggcgg
     3181 cgatgagttc ggtctgctga tccgcaattg cgaactggat gatgcggtgc aactggcggg
     3241 tgtggtgcgt gacaaaatcg ccgagcatca atttgtgtgg gatcgccggc cctaccatgt
     3301 gggcgtcagt attggcctgg tccgcatcgg gcaggaaacc acgcatctga ccgcattgat
     3361 gagtgcagca g
//
LOCUS       DASUEG010000549         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1374307, whole genome shotgun sequence.
ACCESSION   DASUEG010000549 DASUEG010000000
VERSION     DASUEG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374307"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFX11_10345"
     CDS             <1..855
                     /locus_tag="VFX11_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX4939066.1"
                     /translation="RNLAVSFDGRYAIAANYLPRNLVILDAETLAPLQVIDAGSRVSA
                     VYTAPPRNSFIAAMKDLKEVWEIPYDKPSADGSFSIRKIATGDYLDDFFFDQDYTRLI
                     GASRDGKTGQVLDLASGAKVADIPLGGMPHLGSGITFQSQGKTVLATPHLNEGKVTVI
                     DMSNWQVIKQIETLGPGFFMRSHDNSPYAWVDVFFGPNKENVHVIDKETLTIRQTLRP
                     APGKTAAHVEFTKDGRYALLSIWDDDGAVIVYDSKTLKEIKRIPMKKPSGKYNVYNKT
                     RYQAGTSH"
     gene            1128..1559
                     /gene="rplM"
                     /locus_tag="VFX11_10350"
     CDS             1128..1559
                     /gene="rplM"
                     /locus_tag="VFX11_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX4939067.1"
                     /translation="MKTTISAKPESVERDWYVVDASGKTLGRLATEIARHLRGKHKPI
                     YTPHVDTGDYIVVVNADKVQVTGSKRTDKIYYHHTEYPGGIKEISFEKLMVKKPQQAI
                     ETAVKGMMPRNPLGRAMLRKLKVYAGPEHPHTAQQPKELAL"
     gene            1577..1966
                     /gene="rpsI"
                     /locus_tag="VFX11_10355"
     CDS             1577..1966
                     /gene="rpsI"
                     /locus_tag="VFX11_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX4939068.1"
                     /translation="MVETNYGTGRRKTATARVFLKPGTGTISINGRSLDTYFGRETAR
                     MVVRQPLELVQALDKFDIYVTVAGGGGSGQAGAIRHGITRALMDHDETLRSTLRKAGF
                     VTRDSREVERKKVGLRKARKRPQYSKR"
     gene            2180..2779
                     /gene="petA"
                     /locus_tag="VFX11_10360"
     CDS             2180..2779
                     /gene="petA"
                     /locus_tag="VFX11_10360"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX4939069.1"
                     /translation="MSSDGVNEGRRRFLIGATSAVGAIGAVGAAVPFVQSWQPSAKAK
                     TAGAPVVADISKLEVGQRMTVEWRGQPVWILRRSPEAMSSLTKIEESLRDPASDESKQ
                     PEYAKNQSRARQDRGEILVLKGLCTHLGCSPLYRPEVAPADLGADWVGGFFCPCHGSK
                     FDLSGRVYKSVPAPLNLEVPPYYFMSDTLIKIGEDGEAA"
     gene            2779..>3779
                     /locus_tag="VFX11_10365"
     CDS             2779..>3779
                     /locus_tag="VFX11_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX4939070.1"
                     /translation="MSMFQSMMGWVDARFPATKMFEEHMSKYYAPKNFNFWYFFGSLA
                     MLVLVNQLLTGIWLTMMYNPTGEGAFASVEMNMMRDVEYGWLLRYLHSTGASAFFAVV
                     YLHMFRGMMYGSYRAPRELVWIFGMLIYLALMAEGFMGYLLPWGQMSYWGAQVIISLF
                     GAIPYVGDALMEWIRGDYLLSGITLNRFFALHVVALPIVILALVVLHIIALHEVGSNN
                     PDGVDIKKLKDENGVPLDGIPFHPYYTVHDIVGVVVFLAVFLTIVFFFPDGGGYFLEK
                     PNFEPANPLKTPAHIAPVWYFTPFYAILRAIPDKLMGVIAMGAAIAILFVLPWLDRSP
                     "
BASE COUNT          809 a         1087 c         1056 g          827 t
ORIGIN      
        1 cgcaacctgg ccgtgtcctt cgacggccgc tacgccatcg ccgccaacta cctgccacgc
       61 aacctggtga tcctggatgc cgaaaccctg gcgccgctgc aggtaatcga cgccggcagc
      121 cgggtcagcg ccgtctatac cgcgccgccg cgcaacagct ttatcgccgc catgaaggat
      181 ctgaaggaag tctgggaaat cccctatgac aaaccgtccg ctgacggcag tttttctatc
      241 cgcaagatcg ccaccgggga ttacctggac gacttcttct tcgaccagga ttacacccgt
      301 ctgatcggcg ccagccgcga cggcaagacc gggcaggtgc tggatctggc cagtggcgcc
      361 aaggtggctg acattccact cggcggcatg ccccacctgg gctccggcat cactttccag
      421 agccagggca aaaccgtgct ggccaccccc caccttaacg aaggcaaggt aactgtcatc
      481 gacatgagca attggcaggt gatcaaacag atcgaaaccc tcggccccgg tttcttcatg
      541 cgcagccacg acaacagccc ttatgcctgg gtggatgtgt tcttcggccc caataaagag
      601 aacgtccatg tcatcgacaa ggaaaccctg acgatcaggc aaaccctgcg cccggccccc
      661 ggcaaaaccg ccgcccatgt ggagttcacc aaggacggcc gctacgccct gctgtcgatc
      721 tgggacgacg atggtgccgt catcgtgtac gacagcaaaa cgctcaagga aatcaagcgt
      781 ataccgatga aaaagcccag cggcaagtac aacgtctaca acaaaacccg ctaccaggct
      841 gggacaagcc actgatcacg gcagggggcg ctgatcttaa attcggggtt ctggcatcga
      901 tccggcaaaa actgctggtc ttgaagattg attctgctag acttctgcaa ccccgttaca
      961 gtcgtatcaa aaaatggcca ttgattggcg caacagttcg tcaaatcagt gcgatcactc
     1021 attctgtccc tgaaattgct tgtcaagagg gggcggcttg ggtagtattc gcgctcttta
     1081 aaattgacgg gcagacctcg gtttagagtg tttctagggc agattcaatg aaaacaacga
     1141 ttagcgcaaa gccagaatcg gtagaacgtg actggtacgt tgtcgatgca agcggcaaaa
     1201 ctttgggtcg actggcaacc gaaatcgcca ggcacctgcg tggcaagcac aagcctatct
     1261 ataccccaca cgttgacacg ggcgactaca tcgttgtcgt gaacgccgac aaggtgcagg
     1321 taacgggcag caagcgtacc gacaagatct attaccacca caccgaatat cctggtggta
     1381 tcaaggaaat cagcttcgaa aagctgatgg tcaagaagcc ccagcaggcg attgaaacgg
     1441 cagtgaaggg catgatgccc cgtaatccgc tgggccgcgc catgttgcgc aagctgaaag
     1501 tctatgcggg ccctgagcat ccccatactg ctcagcaacc caaagaattg gctctgtaat
     1561 cataacggaa ggcagaatcg tggaaactaa ttacggtaca ggccgtcgca aaactgcaac
     1621 cgcccgtgtg ttcctgaagc ccggtacagg cactatctcc atcaacggtc gttcgctgga
     1681 cacctacttc ggtcgtgaaa ccgcccgcat ggtggttcgt cagccgctgg agctggtgca
     1741 ggcgctggac aagttcgata tttacgtaac cgttgccggt ggcggcggta gtggtcaggc
     1801 cggtgcgatc cgtcatggta ttacccgcgc cctgatggat cacgacgaaa ccctgcgctc
     1861 cactctgcgc aaggctggtt tcgtaacccg tgattcccgt gaagtcgaac ggaaaaaagt
     1921 gggtctgcgc aaggcgcgca agcgtccgca gtactccaag cgctgatatt tctgcgcgaa
     1981 aaacaagctg tatcaaaagc gcccgggtgg tttccatctg ggcgttttcg tttgtggcgc
     2041 ctgtgtttgc gcgctaaagc tttccttgta actcccttgt aatttcatta ctatttgcgg
     2101 cgtttcaaat tatgggtatg ggatagtcgt gtctgttgaa aggcctgctg ttcctggtac
     2161 tgatggggag agaaactgaa tgagttctga cggcgtgaat gagggacggc ggcggttcct
     2221 gattggagcc acctctgcag tcggcgccat cggcgccgtg ggtgcagcag tgccctttgt
     2281 gcaatcctgg cagccgagtg ccaaagcaaa aacagccggc gcgccggtgg ttgctgatat
     2341 cagcaagctg gaagtgggtc agcgcatgac cgtggagtgg cgcggtcagc cagtctggat
     2401 tctgcgccgt tctcctgagg ccatgtccag cctgacaaaa atagaagaaa gtctgcgcga
     2461 ccccgcctcc gatgaaagca aacagcctga atacgccaag aaccagtccc gcgcccgtca
     2521 ggatcgcggt gagatcctgg tgctgaaggg gttgtgtacc catctgggct gttcccccct
     2581 gtatcggcct gaagtagcgc ctgccgatct gggcgcggac tgggttggtg gcttcttctg
     2641 tccctgtcac ggttccaaat tcgatctgtc cggacgcgta tacaaatccg ttccggcacc
     2701 gctcaacctt gaggtgccgc cgtactactt catgagtgat acgctgatca agatcggcga
     2761 agacggggag gcagcctgat gagcatgttt cagagtatga tgggctgggt tgacgcccgc
     2821 ttccccgcga ccaagatgtt tgaagagcat atgagcaaat actatgctcc caagaacttc
     2881 aatttctggt acttcttcgg ttccctcgcc atgctggtgc tggttaacca gctgctgacc
     2941 ggcatctggc tgaccatgat gtacaacccg accggtgaag gcgcattcgc atcggtagag
     3001 atgaacatga tgcgtgacgt cgaatacggc tggctgttgc gctatctgca ttccaccggc
     3061 gcgtcggcct tcttcgctgt tgtttatctg cacatgttcc gcggcatgat gtacggttcc
     3121 taccgcgctc cccgcgagct ggtgtggatc ttcggcatgc tgatctacct ggcgctgatg
     3181 gcggaaggtt tcatgggtta cctgctgccg tggggccaga tgtcctactg gggtgcacag
     3241 gtcatcatct ccctgttcgg cgcgatcccc tatgtgggcg acgcactgat ggaatggatt
     3301 cgtggtgact atctgctgtc cggaattacg ctgaaccgct tctttgccct gcacgtggta
     3361 gcactgccaa tcgtgatcct cgcgctggtt gttctgcaca tcatcgccct gcacgaagtg
     3421 ggatccaaca atcccgatgg cgtcgatatc aagaaactga aggatgaaaa cggcgttccg
     3481 ctcgacggca tccctttcca cccttactac accgtgcacg atatcgtggg cgtggtcgtg
     3541 ttcctggcgg tgttcctcac catcgtgttc ttcttccccg atggcggcgg ctacttcctg
     3601 gagaaaccca actttgagcc agccaacccc ctgaagacgc ctgctcatat tgcacccgtt
     3661 tggtacttca cgcctttcta cgccatcctg cgggccattc ccgacaagct gatgggcgtt
     3721 attgcgatgg gtgccgcgat tgccattctg tttgtgctgc cctggctgga tcgcagccc
//
LOCUS       DASUEG010000550         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_357384, whole genome shotgun sequence.
ACCESSION   DASUEG010000550 DASUEG010000000
VERSION     DASUEG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357384"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFX11_10370"
     CDS             <1..1197
                     /locus_tag="VFX11_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939071.1"
                     /translation="AAPISMMALNPTNCKVTATADVYKCGSTTENVVPDLGISDAEPA
                     LFKSPNVPAGFSSITNAQIANLDVKTIAALTFGIPVTNALYGALQAAQELDTDLDGDN
                     IVEGTSETATQAQILANMPSVSDDQIGALLKGSIGTWDSVSYGGTDLVTAAANAGLPV
                     PANTSVTICRRVDGSGTQAVANAMFLNVPCNTSAGPAAADNTICHSDKGAQNLAEADC
                     TAGGAWARYADQRGLPAGEAVVFENSGSGDVEFCLSDLQDIGTWALGIQSLEKTSTKY
                     SYLKINGAAPTLQNVATGNYMDWASSTMQYRKALTAGIPAPTGNAKRIMDQLRVDFAK
                     PSVLKPLNDTFGSAFGPVGWLSASATPVLPFNPNLPVMQFTRGASGMSTCNAQVARGT
                     FDMKKQ"
     gene            1316..>2003
                     /locus_tag="VFX11_10375"
     CDS             1316..>2003
                     /locus_tag="VFX11_10375"
                     /inference="COORDINATES: protein motif:HMM:NF020068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX4939072.1"
                     /translation="MKRSIAVVCVGALMCAKVVAEEVAPANAPVAVPSQKEAVQPRFA
                     VWEFRVKGNTLLQQTAVELTLTPYLGPDQTFETIQEAASKLERSYRDAGYPTVLVNIP
                     EQDVVGGVVMLEVVEGKVSRVRVSGSRYFLPSEIKQEVGSLQQGQPMHVPSFQADMNR
                     VNSASGDLRVTPVLKPGGTPGTVEVDLRVKDELPLHGSLEVNNHNSLDTTKLRTSASI
                     SYDNLWQKHHS"
BASE COUNT          416 a          603 c          605 g          379 t
ORIGIN      
        1 gccgctccca tcagcatgat ggcgctgaac ccgaccaact gtaaggtgac tgccactgcc
       61 gacgtttaca agtgcggcag caccactgaa aacgtggtgc ccgacctggg tatttccgac
      121 gccgagccgg cgctgttcaa atccccgaac gtccctgccg gcttctcttc catcaccaac
      181 gcccagattg ccaacctgga cgtcaagacc attgcggccc tgacattcgg tattccggtg
      241 accaacgcgc tgtacggcgc actgcaggca gcccaggaac tggacactga cctggatggc
      301 gacaacatcg tggaaggcac cagcgaaacc gcaacccagg cccagattct ggccaacatg
      361 ccgtcggtga gcgatgacca gatcggcgcg ttgctgaaag gctccatcgg tacctgggac
      421 tccgtttcct acggtggtac cgacctggtg accgcggccg ccaacgccgg tctgccggtt
      481 cctgccaaca cctccgtcac catttgccgc cgtgtggacg gttccggtac ccaggccgtg
      541 gccaatgcca tgttcctgaa tgtaccctgc aacacgtctg ccggtcctgc cgctgcagac
      601 aacaccatct gccacagcga caaaggtgcg cagaacctgg cggaagcgga ttgcaccgcc
      661 ggtggtgcct gggcacgtta tgctgaccag cgcggcctgc cggctggtga ggcggtcgtg
      721 ttcgagaaca gcggctctgg cgacgttgaa ttctgcctga gcgatttgca ggacattggt
      781 acctgggcac tgggcatcca gtctctggaa aaaaccagca ccaagtacag ctacctgaaa
      841 atcaacggtg cagccccgac cctgcagaac gttgccaccg gcaactacat ggactgggct
      901 tccagcacca tgcagtaccg caaggcactg accgccggta tacctgctcc aaccggcaac
      961 gccaagcgca tcatggacca gctgcgggtg gacttcgcca agccgtccgt gctgaagccg
     1021 ctgaacgaca ccttcggttc cgcgttcggt ccggtgggct ggttgtccgc ttccgccacc
     1081 ccggttctgc cgttcaaccc gaacctgccg gtcatgcagt tcacccgcgg cgccagcggt
     1141 atgtctacct gtaacgccca ggttgcccgc ggcaccttcg acatgaagaa gcaataatct
     1201 ggcagtctaa ctgtcatatt ggcgcagtaa agttggccag ccctgcaatg gggctggcct
     1261 ttttcgtttt tgtcatttgc aaaaatcaat ttcaaccaat cctgggggat ggggcttgaa
     1321 gcgttcgatc gccgttgtct gcgtgggtgc actgatgtgc gcaaaagtgg tggctgaaga
     1381 agtagcgccg gccaatgcgc cggtggcagt gcccagtcaa aaagaggccg tgcagccgcg
     1441 gtttgccgtc tgggaatttc gcgtcaaggg caacacgctg ttgcagcaga ccgccgtcga
     1501 actgacgctg acaccttatc tggggccgga ccagacgttt gaaaccattc aggaagcggc
     1561 cagcaagctg gaacgcagtt atcgcgatgc gggatacccc accgtgctgg tgaatattcc
     1621 ggagcaggat gtggtaggtg gcgtagtgat gctggaggtg gtggaaggaa aagtgtcgcg
     1681 ggtgcgggtg agcgggtcgc gctatttcct gcccagcgag atcaagcagg aagtggggtc
     1741 gttgcagcag ggccagccga tgcatgtccc cagttttcag gccgatatga accgggtgaa
     1801 cagcgcgtcc ggggatctgc gggtgacgcc ggtgctgaag ccaggcggta ccccgggcac
     1861 ggtggaagtg gatctgcggg tgaaggatga attgccgctg cacggttcac tggaagtgaa
     1921 caaccataat tccctggata caaccaaact gcgcacctcg gccagcatca gttacgacaa
     1981 tctgtggcag aagcatcaca gca
//
LOCUS       DASUEG010000551         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1107639, whole genome shotgun sequence.
ACCESSION   DASUEG010000551 DASUEG010000000
VERSION     DASUEG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107639"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFX11_10380"
     CDS             <1..627
                     /locus_tag="VFX11_10380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939073.1"
                     /translation="QPWWDVWLRVLYDLAAIPLSALFFLLFHLLEREKRQLEYWVRTD
                     ALTGLTSRAAFMQQFEVECHRHARSGHSLCLLLCDIDHFKQVNDRWGHPAGDEVLAHL
                     GSIMTHSIRPSVDVAARFGGEEFAILLPETDLQQATQVAEQIGTQLRAHVFQANSHSF
                     QVTLSIGIAQSNDGDGELALRVADDNLYAAKRGGRDRVISAMAPAFAT"
     gene            718..1653
                     /locus_tag="VFX11_10385"
     CDS             718..1653
                     /locus_tag="VFX11_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939074.1"
                     /translation="MTTLLRRTAFLSQKWQDSAFLITRTLGRLVARLELKQQDIANGC
                     PLFYYDNNNNAATETLVLVHGFNADKDHWIAMALHLSRYRVIIPDLGGHGQSWYDPAL
                     TYDIPFYARQLNQLLNALCIDRFHLLGNSMGGWVAAWYAHAHPAEVKTLCLMNACGVA
                     SPVLSPFFKALAQRRNPFFYSSPAEYAELLDMAVANPRTIPPSIRAVNYRHGLVRMEQ
                     ARKLFADITNPDQTYLAPAQQVDPLLGQIRQPTLVLWGDRDGIMDPTMANVYSRGLPN
                     NEKIILPNVGHTPMLEQPARVARCYKAFLQRHAAD"
     gene            complement(1681..2484)
                     /locus_tag="VFX11_10390"
     CDS             complement(1681..2484)
                     /locus_tag="VFX11_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939075.1"
                     /translation="MLIQPILHRPAFMYPWEKRTLFLGRLPEPVTAAYGAATLVISLG
                     NPIDLKVGGRIIHSASFLVPAGVRVSINAGLQPVAIFHLDVLGEDFQRFQGCFRHQSR
                     GMTVSHDVRDEADFHALLNQLYRQPGASAVTYRQLDGLLDYSFNRAGNPSRIDPLVQE
                     AVDHIKQNYQDNIPVEELAQRVNVSTPTLIRKFKQQSGVPIRRFRLWHRIYESMLFIS
                     AGQNLTDAALSAGFSDSSHYTHTLQAMWGVSPSNLFARDIRAEIVPPQE"
     gene            2793..>3129
                     /locus_tag="VFX11_10395"
     CDS             2793..>3129
                     /locus_tag="VFX11_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939076.1"
                     /translation="MKFNISALTRLTAALLIAMSTSATFAGDDTRTAVGSAIGGGVGA
                     AIGQEVGGTNGAIIGGAVGGAAGGAASAKGSAKTGAAIGGAIGGASGVAVGGHVGGST
                     GQIVGGAVGA"
BASE COUNT          623 a          929 c          928 g          649 t
ORIGIN      
        1 cagccctggt gggacgtctg gttgcgggtg ctgtacgatc tcgccgcgat tcccctcagc
       61 gcgctgttct ttctgctgtt tcatctactg gagcgggaaa agcggcaact ggaatattgg
      121 gtgcgtaccg atgctctgac aggactgacc agccgggccg cgttcatgca acagttcgag
      181 gtggaatgtc accgccacgc gcgcagtgga cattcgctct gcctgttgct gtgcgacatc
      241 gatcatttca aacaggtcaa cgaccgttgg ggccatccgg ccggtgacga agtgctggcc
      301 cacctgggca gtatcatgac gcacagcatc cgtccgtcgg tggatgtggc agcccgtttc
      361 ggcggcgagg aattcgcgat cctgctgccg gaaacggatt tgcagcaggc cacacaggtc
      421 gccgagcaga tcggcacgca attgcgcgcc catgtgttcc aggccaacag ccattccttt
      481 caggttacgc tgagcatcgg cattgcccag agtaacgatg gtgacggcga actggcgctg
      541 cgggtagccg atgacaatct ttacgccgcc aaacgcggtg gacgtgatcg ggtcatcagc
      601 gcgatggcgc ccgcattcgc aacctgatca atacatacaa gacaggtcag atgccgcacc
      661 ataagatccc gctcaatccc ctccttcaat taacgctgtc tgccggatgg aatcacaatg
      721 acgacgctct tgcgccgaac cgcatttttg tcgcaaaaat ggcaggattc cgctttcctg
      781 atcacccgca ccctgggccg cctggtagcc cggctggaac tgaaacaaca ggacattgcc
      841 aacggctgcc cgcttttcta ctacgacaac aataacaacg cggctaccga aaccctggtg
      901 ctggtgcacg gtttcaacgc cgacaaggac cactggattg cgatggcgct gcatctttcc
      961 cgttaccgcg tgatcattcc ggatctcggt ggccacggtc agtcctggta tgatcctgcc
     1021 ctcacttacg acattccgtt ttacgcccgc cagttgaacc agttgctgaa cgctctctgc
     1081 atcgaccgct ttcacctgct tggaaattcc atgggcggct gggtggcggc ctggtacgcg
     1141 cacgcgcatc ctgccgaagt gaaaacactg tgtctgatga atgcctgcgg cgtggcatcg
     1201 ccggtgctca gtccgttttt caaggcgctg gcacaacggc gcaacccgtt tttctattcc
     1261 agcccggcgg aatatgctga attgctggat atggcggtag cgaatccacg caccattccc
     1321 ccatccattc gcgccgtgaa ttaccgccac gggctggtgc gcatggagca ggcccgcaaa
     1381 ctgttcgccg atatcaccaa tcctgatcag acctatctgg cgcccgcgca gcaggtggac
     1441 ccgttgctgg ggcagatccg ccagcccacg ctggtgctgt ggggcgaccg cgacggcatc
     1501 atggacccca ccatggccaa tgtgtacagc cgcggcttgc cgaacaatga aaaaattatc
     1561 ctgccaaacg tggggcacac gcccatgctg gaacagccgg cacgggtagc ccgctgctac
     1621 aaggcatttt tgcagcggca cgcggctgat tgagggaatg cactttgtgc ggtatgccgg
     1681 ttattcctgc ggcggcacga tctcggcgcg aatatcccgc gcgaacagat tgctggggct
     1741 gacgccccac atggcctgca gtgtgtgggt gtagtgggat gaatcggaaa agccagcgct
     1801 cagcgcggca tcagtgagat tctggcccgc actgataaac agcatggatt cgtaaatccg
     1861 gtgccacagg cggaagcgcc ggattggcac accggattgc tgcttgaatt tccggatcag
     1921 ggtgggcgtc gatacattca cccgctgtgc cagttcctcc accggaatat tgtcctgata
     1981 attctgtttg atgtggtcca cggcctcttg caccagcgga tcgatcctgg aggggttgcc
     2041 ggcgcgattg aaactgtaat ccagcaaacc atccagctgc ctgtatgtta ccgctgacgc
     2101 gccgggctgg cggtacagct ggttcagcag cgcgtgaaaa tcggcttcgt cgcgcacatc
     2161 gtgggacact gtcatgcccc ggctctgatg ccggaagcaa ccctgaaacc gttggaaatc
     2221 ttctcccagc acatccaggt ggaaaatcgc caccggttgc agaccggcgt tgatcgacac
     2281 ccgcacgccc gccggaacca ggaagctggc actgtgaatg atcctgccgc ccactttcaa
     2341 atcaatcgga ttgcccaggc tgatcaccag ggtggctgcg ccataagctg ccgtcaccgg
     2401 ttccggcaga cggccaagga acagggtgcg cttttcccac ggatacatga atgcgggccg
     2461 gtgcagaatg ggttgaatca acatggtgat cgggtccggg ctaaacattc ttattatagt
     2521 aaacaggccg ccagaggatg ccgcagccga gcgatattta tcactgccgg tctgttcgtc
     2581 atggccttgt tttttcagtg cctatggcag gcgtggacgg ctgccgttcc aaactgtgcc
     2641 aaacaggctt agtcaaatct ggcatgcgta ttttatgaag cgcatcatat tccgttgaag
     2701 ccggtttgcc cggacagagt gtgtttgcaa caatgatgct tactgtaaag gcgcactcac
     2761 gccgcccacc tcatgtaaca cccggagcat ctatgaaatt caatatatcg gcactgacac
     2821 ggctgacggc cgccctgttg atcgccatga gtaccagcgc caccttcgcc ggcgacgaca
     2881 cccgcaccgc cgttggcagt gcaattggtg gtggtgtcgg cgcggccatt ggccaggaag
     2941 tgggcggtac caacggcgcc atcatcggtg gcgcggtggg tggtgctgcc ggcggcgcgg
     3001 ccagcgccaa gggttccgcc aaaaccggcg cggctatcgg tggtgccatc ggtggcgcca
     3061 gcggcgtggc agtgggtgga catgtgggtg gttccaccgg ccagatcgtg ggtggcgcgg
     3121 ttggtgcgg
//
LOCUS       DASUEG010000552         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2060884, whole genome shotgun sequence.
ACCESSION   DASUEG010000552 DASUEG010000000
VERSION     DASUEG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060884"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="zapE"
                     /locus_tag="VFX11_10400"
     CDS             <1..806
                     /gene="zapE"
                     /locus_tag="VFX11_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX4939077.1"
                     /translation="ELAGDVRVLCFDELFVSDIGDAMLLGRLFQGMFEQGVVIVATSN
                     QAPAQLYAGGFNRQRFLPAIDAIEQHMRIVQVDGGQDHRLHPGRPQQRYWVSQPGQSG
                     AMNAVFAQLAAGQPTDTGEIEVGSRAIKTVRHSRSVLWCRFADLCQIPLSALDFIALC
                     DRFSALMLSEVPRLGGRQHEAYIARGTEDGVERVQAGDRLLPDLARNDDSVRRFIALV
                     DECYDRRVPLYLEAAAPLDELYTEGYLEFPFRRALSRLKEMQLERFGME"
     gene            complement(948..1024)
                     /locus_tag="VFX11_10405"
     tRNA            complement(948..1024)
                     /locus_tag="VFX11_10405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Pro,seq:ggg)
     gene            complement(1138..1497)
                     /locus_tag="VFX11_10410"
     CDS             complement(1138..1497)
                     /locus_tag="VFX11_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4939078.1"
                     /translation="MLEPSNNNELPVIPGKRYFTIGEVSELCDVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQRQDVIMIRQIRSLLYDQGYTIGGARQKLSGEDARDDVTKSSQLI
                     RQMITELEDVLFVLKDH"
     gene            complement(1475..1780)
                     /gene="ihfA"
                     /locus_tag="VFX11_10415"
     CDS             complement(1475..1780)
                     /gene="ihfA"
                     /locus_tag="VFX11_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX4939079.1"
                     /translation="MSALTKADLAEKLFEELGLNKREAKEMVELFFEEIRASLESNEH
                     VKLSGFGNFDLRNKRQRPGRNPKTGEEIPISARRVVTFRPGQKLKQRVEAYAGTKQQ"
     gene            complement(1783..>2236)
                     /locus_tag="VFX11_10420"
     CDS             complement(1783..>2236)
                     /locus_tag="VFX11_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX4939080.1"
                     /translation="PGQTAAILRHGERVGHVGALHPVLQKSLDIQVKIYLFELVLAPV
                     SRGILPRFKELSKYPEVRRDLAVIVDEKISAQAILKNVRESAGVLLTDLALFDVYQGA
                     NLEAGKKSIAFGVTLQDPSRTLQDEDVNPVIDRIVASLRENFKVTLRE"
BASE COUNT          493 a          631 c          616 g          496 t
ORIGIN      
        1 gagaactggc cggtgacgtc agggtactgt gtttcgatga actgttcgtc agcgacatcg
       61 gcgatgcaat gctgcttggc cggctgtttc agggcatgtt cgagcaaggc gtggtgatcg
      121 ttgccacctc caaccaggcg cccgcgcaac tgtatgccgg tggcttcaac cgccagcgct
      181 ttctgccagc cattgacgcc atcgagcagc atatgcgcat tgtccaggtg gatggaggcc
      241 aggatcatcg ccttcacccg ggacgcccgc agcagcgcta ctgggtcagc cagcccgggc
      301 agagcggcgc catgaatgcg gtattcgctc aactagcagc gggccaaccg acagacacgg
      361 gagaaataga agttggctca cgggcaataa aaacggttcg ccatagccgg tcagtgctct
      421 ggtgccgctt tgcggatctg tgccagattc cattgtcggc gctggacttc attgctctgt
      481 gcgaccgctt ctcggccctg atgctgagtg aagtacctcg cctcggcggc cggcagcacg
      541 aagcctacat cgcccggggc accgaagacg gcgtcgagcg ggtgcaggcc ggtgaccggc
      601 tgttgccgga tctggcacgc aacgacgaca gcgtgcgccg cttcatcgcg ctggtggacg
      661 aatgctacga ccgccgtgta ccgctctatc tggaagccgc ggcgccactg gatgagttgt
      721 acacggaggg ttatctggaa tttccgttcc gacgtgcgct gagccggttg aaggagatgc
      781 agctggaaag gtttggaatg gagtgagccc gctcagtatc ggcgggaaat actcactgca
      841 acggaaatgg actcgggcac gggggcacta attgacttac agaaagcgaa attccgggac
      901 ataaaaaagc cggttagcgt aaactaaccg gctttctttg tagaatctgg tcgggacgga
      961 aggatttgaa ccttcgaccc cttgcacccc atgcaagtgc gctaccaagc tgcgctacgc
     1021 cccgaattgt tttcaattca agatgttaga aacgatcaga cccttgattg aggtggcaaa
     1081 tattacagga aaagagacaa atagcaaacg gtttttagcc aaccgtttgc tatcagatca
     1141 gtgatccttg agcacgaaca gaacatcttc cagctcagtg atcatctggc ggatgagctg
     1201 ggacgatttg gtgacgtcgt cacgggcgtc ttcgcccgac agcttctggc gagcgccacc
     1261 gatggtataa ccctgatcgt acagcagact gcggatctgc cggatcatga ttacatcctg
     1321 acgctggtaa taacgacggt tgccgcgacg cttgaccggc ttcaactggg taaattcctg
     1381 ctcccagtaa cgcaaaacgt ggggcttcac gtcacagagt tcgctcactt ccccaatcgt
     1441 aaaatagcgc ttgcccggta tgactggcag ttcgttattg ttgcttggtt ccagcatagg
     1501 cttccactcg ctgcttcaac ttctggcctg ggcggaaggt caccacgcga cgggcggaaa
     1561 tcggaatttc ttctccggtc ttgggattcc ggccgggacg ctgccgcttg ttgcgcaggt
     1621 cgaaatttcc gaagcccgac agtttcacat gctcattgga ctcaagcgac gcacgaatct
     1681 cttcgaaaaa gagttcgacc atttccttgg cctctcgctt gtttaaccca agttcctcaa
     1741 atagcttttc agctagatca gccttggtca acgcactcat gcctattccc tcaatgtgac
     1801 tttgaagttc tctcgcagcg aggccacaat ccggtcgatc actggattga cgtcctcatc
     1861 ctgaagcgtt cgggacgggt cctgcagggt cacgccgaaa gcaatgctct ttttgcccgc
     1921 ttccagattg gccccttggt acacgtcgaa cagcgcgagg tcggtcaata aaacaccagc
     1981 cgattcacgc acatttttca gtatagcctg cgcagaaatc ttttcatcaa caattacggc
     2041 taaatcacgt ctaacttccg gatatttcga caattctttg aaccgcggca aaattccccg
     2101 gctcacggga gccaaaacga gttcgaatag gtagattttg acctggatat caaggctctt
     2161 ctgcagcacc ggatgcaggg cacccacatg gccgacccgc tcaccgtgac gcagaatcgc
     2221 agccgtctgc cccggg
//
LOCUS       DASUEG010000553         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1825252, whole genome shotgun sequence.
ACCESSION   DASUEG010000553 DASUEG010000000
VERSION     DASUEG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825252"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="VFX11_10425"
     CDS             complement(<1..1961)
                     /locus_tag="VFX11_10425"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX4939081.1"
                     /translation="MSLRNSPPLEIEFALIRIVGNSLPPRHARDEMLQHLRFILQHEP
                     DLEGCEKSWILNRIADERLADTCAALIEAAGQRVIRLPFDIDAYAQRFNDLSGVSPRL
                     VSAAALKDAATFEPATFALEWKYRYKSLYAISLNAARNLALSLGRGRAQWTLPWDGAC
                     FVTRQAWSGIRQLAQRDVTALHLVVPLTRVTDAQALLQPHFQPVTFTEPQLAFRHDSQ
                     DRFDEQLRYGNRNKAEMLVRLGVPGPWHTWRPASWDVQPVRSSPERGRFVQGGWVARL
                     PAGCDPQIDSNDPLRWRSRFAGVAGLCQQLDVRVVAKHNRAGRLLCFDEAALRNPDAA
                     VVRYLQEQAEIAMARPIPSVLDKTGCAPSGDRQDYLSMAPYLHRDAAQQGVELRDGVR
                     TAESTPGTEESRSNDRGTLERMIHDVCATALAGTVVGDSACRDHAASMLRAWFIDPRT
                     RMHPHMRFAQCVPGSRDGGQPWGVVDFRNLWPLLDAVTLLRRAGALSLPEHGEIQSWF
                     AAFLDDITSRAGAQAPNNIAVWHDLVAAAIAAFLGRNGQAAQILSDVPLRMGAQLSPF
                     AVPHLELQRSRPLHYGLFLAQGLVHLACLGRALGIDLWRYQASQARSVAMLIRFLALN
                     RSLLDDYAADAAAFDDRIAQMAMLVPADAV"
     gene            complement(2088..>2276)
                     /locus_tag="VFX11_10430"
     CDS             complement(2088..>2276)
                     /locus_tag="VFX11_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939082.1"
                     /translation="ETIYALLSEPQLERWQQSREEVLRETGMVPHRAGTDALIIQKTY
                     LFACLPTAERNLSKKGAA"
BASE COUNT          463 a          766 c          665 g          382 t
ORIGIN      
        1 actgcatccg ctggcaccag catcgccatc tgtgcgatgc ggtcatcaaa cgcagcggcg
       61 tcagcggcgt aatcatccag caacgaacga ttgagcgcca gaaagcggat cagcatggcc
      121 acgctgcgcg cctgtgacgc ctgataacgc cacagatcaa tacccagcgc tcgtcccaga
      181 cacgccagat gcaccagccc ctgcgccagg aacaggccgt aatgcagcgg acggctgcgc
      241 tgcaattcca ggtgcggcac ggcaaacggc gacaactgcg cacccatgcg cagcggcaca
      301 tccgacagga tctgcgcggc ctggccgtta cgtccaagga atgccgcgat cgctgcagcg
      361 accagatcgt gccacaccgc gatgttgttg ggagcctgcg caccagcacg ggaagtaatg
      421 tcatccagaa aggcggcaaa ccacgactgg atttcgccgt gctcgggcaa cgacaacgcc
      481 ccggcccgcc gcaacagcgt cacggcatcc agcaggggcc acagattgcg aaaatccaca
      541 acgccccacg gctgcccgcc atcacgactg cccggcacgc actgcgcaaa gcgcatgtgc
      601 ggatgcatgc gcgtgcgcgg atcgataaac caggcccgca gcatgctggc agcatgatcg
      661 cggcatgctg aatcgcccac cacggtaccc gccaatgccg tcgcacacac atcgtgaatc
      721 atgcgctcca gcgtgccgcg atcattgctg cggctctctt ccgtgcccgg tgtggattcg
      781 gccgtgcgca cgccgtcacg gagttcgact ccctgctgcg ccgcatcacg gtgcagatag
      841 ggcgccatgc tcagataatc ctgccggtcg ccgctgggcg cacaaccggt tttgtccagc
      901 acggagggaa tgggccgcgc catcgcgatc tctgcctgtt cctgcaaata ccggacaacg
      961 gcggcatccg ggttgcgcaa cgccgcctca tcaaaacaca gcaatcgccc tgcccgatta
     1021 tgcttcgcca ccacacgcac atcgagctgc tggcacaagc cagccactcc ggcgaaccgg
     1081 gagcgccagc gcaacggatc attggaatcg atctgcggat cgcacccggc tggcaaccgt
     1141 gccacccacc cgccctgcac aaagcggccc cgctcgggac ttgaacgcac cggctgcaca
     1201 tcccagctgg ccggccgcca ggtgtgccaa ggtcccggca cccccaggcg caccagcatt
     1261 tccgccttgt tgcgattgcc ataacgcaac tgctcatcga aacggtcctg gctgtcatgt
     1321 ctgaatgcca gttgcggctc ggtgaacgtc acaggctgaa agtggggctg cagcaaggcc
     1381 tgcgcatccg tcacgcgtgt cagcggtacc accagatgca atgccgtcac gtcccgctgc
     1441 gccagctgac ggattccgga ccacgcctgc cgggtaacaa agcaggcgcc atcccacggc
     1501 aaggtccact gcgcccgccc gcggcccagc gacagtgcca gattccgcgc cgcattcagg
     1561 ctgatcgcgt acaggctctt gtaacggtat ttccattcca gcgcgaaagt ggcaggttca
     1621 aacgtggcgg catccttcag cgccgctgcg ctcaccagcc tgggcgatac gccagacaga
     1681 tcattgaaac gttgtgcata ggcatcgata tcaaacggca ggcggatgac acgctggcca
     1741 gcggcctcga tcagtgcggc gcaggtatcg gccaggcgtt cgtcagcaat gcggttcaga
     1801 atccagctct tctcgcaccc ttccagatcc ggttcatgct gcaggatgaa ccgtagatgc
     1861 tgcagcattt catccctggc atgccgcggc ggcaggctgt tgcccacaat gcggatcagc
     1921 gcgaattcaa tttccagagg ggggctattc cgaaggctca tcaactgcat cctgtgcgtg
     1981 gcatgcggcg attatcaccg gccagatgcc ggcttgccaa agcagacctg ctgtgtggat
     2041 gatccaaggg gccaattagg aagggaagca gggcgcagcc cgataactca ggccgcgcct
     2101 tttttgctga gattgcgctc tgctgtgggc aggcaggcga acaggtaggt tttctggatg
     2161 atcagggcgt cggtaccggc acgatgcggc accatgccgg tttcccgcaa cacttcctcg
     2221 cgactttgct gccagcgctc cagttgcggc tcgctcagca gggcataaat ggtttc
//
LOCUS       DASUEG010000554         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_943442, whole genome shotgun sequence.
ACCESSION   DASUEG010000554 DASUEG010000000
VERSION     DASUEG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943442"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="VFX11_10435"
     CDS             complement(<1..2216)
                     /locus_tag="VFX11_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4939083.1"
                     /translation="MNQRIAAFLVRHRLWLFLASLLVVGVAATGFSRLEYVADYKNFF
                     SPDDPHLRAFEDLQDTFSRVDNVVFVLEPKNGKVLSPEGIRALQWITEAGWKLPFSIR
                     MDSLANFQHTRAEEDELIVEDLVPADFDGSAASIDRIARIIASEPTVSKRTLTPDGAL
                     TLAMVTLELPKDQTTALEQLMEGPGGVNELIEQFQAAHPEVTVHVTGVSVSNYYLGIV
                     SARDMNVLMPVLLLVVLVLVGVMTRSVANTFLTLFVVIVSVVTTIGCVGLLGFPLNNV
                     SSVAPMVILTLAVAECVHLLCYYSIKLREGHGKEDAMRLSLESNLRAIFFTSFTTAVG
                     FMGMNTIDSPPFVQFGYIASFGIVMAYVFGHTMLPQLAIWLTRAHTGPIEQHSDTVHS
                     ATADWVIAHPRRVFFGTLLVSFAISIGMVLNDMNDDNVGYFDRDMPLRIAIERAEQHG
                     MGMNFIEYSLDAGENYGITDPAYLAKVEKFIQWAQQQPEVINVSSFTNVLKRLNQNMH
                     GDDPAYYTLPESRELASQYLLMYEMSLPVGMDLNNQIDTGKSALRITLSTRMMKAKQN
                     LALEERVQDWMAANIPELQTHGASPTIMFSHIGQNSIRSVMTGSISAIAIICLCMMFA
                     FGSFRLGLMALLPNIFPSTIALGLWGLFVGEVNMAVAVIFTISSGIIVDDTIHLFSKF
                     SDGLRKGLNVDDSIRYSFAHAGRGVLITTVVLCAGFAMLGFSDFNVNRTLGILVAGT"
BASE COUNT          511 a          695 c          731 g          459 t
ORIGIN      
        1 gtgcccgcca ccagaatgcc aagggttcgg ttcacgttga aatccgagaa acccagcatc
       61 gcgaacccgg cgcacagcac cacggttgtg atcaacacgc cgcgtccggc atgggcaaac
      121 gagtagcgga tactgtcgtc cacattcagg cccttgcgca ggccgtcgga gaatttcgag
      181 aacaggtgaa tggtatcgtc aacgatgatg ccggacgaaa tggtgaagat caccgccacc
      241 gccatgttga cttcacccac gaacaggccc cacaggccca gcgcgatggt ggacgggaaa
      301 atattcggca gcagtgccat cagacccagc cggaaggagc caaatgcgaa catcatgcac
      361 agacagatga tggcgatggc ggatatcgag cccgtcatca cgctgcgaat gctgttctgg
      421 ccgatatggg aaaacatgat ggtggggctg gcgccgtggg tttgcagttc gggaatattg
      481 gcggccatcc agtcctgtac gcgctcctcc agcgccagat tctgctttgc cttcatcatg
      541 cgcgtgctga gggtgatgcg cagcgccgat ttgccggtgt cgatctggtt gttcaggtcc
      601 atgcccactg gcagcgacat ttcgtacatc aacaggtact gcgatgccag ctcgcgggat
      661 tcgggcaacg tgtagtacgc agggtcatcg ccgtgcatat tctgattgag ccgcttcagc
      721 acattggtga aggacgagac gttgatcact tccggttgct gctgggccca ctggatgaat
      781 ttttccactt tggccagata ggccggatcc gtgatgccgt aattctcacc ggcgtccagc
      841 gaatactcga tgaaattcat gcccatgccg tgctgctccg cccgctcgat ggcgatgcgc
      901 agtggcatgt cgcggtcgaa atagcccacg ttgtcgtcat tcatgtcatt cagcaccatg
      961 ccgatgctga tggcgaagga caccagcagc gtgccgaaga acacccgccg gggatgggcg
     1021 atcacccagt cggcagtggc gctgtgtacg gtatcgctgt gctgctcgat gggcccggtg
     1081 tgggcgcgcg tcagccagat cgccagttgt ggcagcatgg tgtggccgaa cacataggcc
     1141 atgacaatgc cgaatgatgc gatgtagccg aactgcacga acggtggcga gtcgatggtg
     1201 ttcatgccca tgaaacccac tgccgtggtg aagctggtga agaaaatcgc gcgcaggttg
     1261 ctttccaggc taagtcgcat ggcgtcttcc ttgccgtgtc cttcccgcag cttgatcgag
     1321 taatagcaga gcaggtgcac acattcggcc accgccaggg tcaggatcac catcggcgcg
     1381 acggaactga cattgttgag cggaaaaccc agcagcccca cgcagccgat ggtggtgacc
     1441 acggacacga tcaccacaaa cagggtgaga aaggtgttgg ccaccgagcg cgtcatgacg
     1501 cccaccagta ccagcaccac cagcagcagt acgggcatca ggacattcat gtcgcgggcg
     1561 gagacgatgc ccaggtaata attggatacc gacacgccgg tgacatgaac cgtcacctcc
     1621 gggtgggcgg cctggaattg ctcgatcagc tcgttgacgc cgccgggacc ttccatcagc
     1681 tgttccagtg ccgtggtctg atcttttggc aattccagcg tcaccatggc caaggtgagt
     1741 gcgccatccg gtgtcagggt gcgtttgctc acggtgggtt cgctggcaat gatgcgggcg
     1801 atgcggtcga tgctggcagc gctgccatcg aaatccgccg gcaccagatc ttccacgatc
     1861 agctcatctt cctccgcgcg ggtgtgctgg aaattggcga gggaatccat gcggatcgaa
     1921 aatggcagtt tccagcctgc ctcggtgatc cactgcaggg cgcggatgcc ctccggtgac
     1981 agcaccttgc cgtttttcgg ttccagcacg aataccacgt tgtccacgcg gctgaaggtg
     2041 tcctgcagat cctcgaatgc ccgcaggtgc ggatcgtcgg gtgaaaagaa attcttgtag
     2101 tcggccacgt actccagacg ggagaagccg gtggcagcca cgcccaccac cagcaggctg
     2161 gccaggaaca gccacagccg gtggcggaca agaaaagcag caatccgttg attcatgacg
     2221 acagactccc cgacgtgtca gcgaaacaga agcagcctgc gctggcagga tgaaatgctg
     2281 cgcaggttaa acacgctgtt tcgcgatgtc catgccgatt tggtaaatgc aagcccgtga
     2341 cagcgcccca gcggagcatc cgtcagaacg ccaggcacgg gccggccgct ggcctg
//
LOCUS       DASUEG010000555         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1244162, whole genome shotgun sequence.
ACCESSION   DASUEG010000555 DASUEG010000000
VERSION     DASUEG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244162"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="VFX11_10440"
     CDS             complement(<1..1696)
                     /locus_tag="VFX11_10440"
                     /inference="COORDINATES: protein motif:HMM:NF044718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX4939084.1"
                     /translation="MTIRRLLLVVLLGLFLAACSESTDQAASGGKGAPVESLETLRER
                     FAGAELTVLDVSEREWQGKNAIAVTLSVPLDPKGDFQRFLAVSQRQGATVDGAWILAD
                     DRRTLYFPASAPRTDYEVTVHRGLPALTGAELKESVTRQLTTRDVRPAVGFASEGSVL
                     PAHLTNGIPITAINVKEVNIDFHRLDQKHIADFTRHLSESYQNRFYRYDQLRSWGTLA
                     YSGRFDLNPPDNTLVKQSIPVEDIPQLQEPGFYVAVMDEAGDYDYRRYVTYFVVTDIG
                     LHARQYPGELVVLASSLADGSALDGISVAIMDAQGNVVQERETSPEGEARFNGDINTG
                     SYLLARSGKQIALLQLQGPALDLSEFDLGNRPQLPQEAFVYTPRDLYRPGETIDFSAL
                     LRDGDGFMVQNQPLNIEIHQPDGQIVQQALLQPQDMAYYLYRFTLPGNAARGNWRFDV
                     TNLAGGNTSYEFKVEDFLPERMALTFNDGQQEPLQFDAEEPVRIPVKGMYLYGAPASG
                     NRLSGTAQVSLLREALPQYKGYLFGDETEAQAAARFDLPDQFLDEAGNTIVNIDSRW"
     gene            1901..>3002
                     /locus_tag="VFX11_10445"
     CDS             1901..>3002
                     /locus_tag="VFX11_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX4939085.1"
                     /translation="MNRFRSLFLLSIPVLAMGCVFLVYGLLSLLLFRDQLHIPFFELA
                     AGTLLAGIAGLFGISRKLENISYRESLLFVTLTWSMLGALGGLPFVLINGLSFTDATF
                     EAISGLTTTGATTITGLDGLPKTLLMYRQFLHWMGGLGVIIFVVAIMPMLNIGGMRLY
                     KAETPGPVKGEKLAPRISHTARYLWYVYVAITLACIAAYWLAGMNLYDAVAHAFATVS
                     TGGFSTHDASISYFNSPAIELVTIVFMLLGAMNFGLHFMVYNSRRLSLYWRDEETRWF
                     FGILLGAILLIALALWWEGKFDALTSLRHSAFQVASFITSTGYFSTDYTVWPGAVILL
                     LIVLQYIGGCAGSTAGGNKVVRNAITMKAIFLE"
BASE COUNT          544 a          932 c          901 g          625 t
ORIGIN      
        1 cccagcgtga atcgatgttg acgatggtat tgccggcttc atccaggaac tggtccggca
       61 gatcgaaacg ggcggcagcc tgcgcctcgg tttcatcgcc aaacaggtag cctttgtatt
      121 gcggcaatgc ttcccgcagc aggctcacct gcgccgtgcc actcaggcga ttgcccgatg
      181 cgggcgcgcc gtacagatac atgcctttga ccggaatgcg caccggttct tccgcgtcga
      241 actgcagcgg ttcctgctgg ccgtcgttga aagtgagcgc catccgttcc ggcagaaaat
      301 cctccacctt gaattcgtag ctggtgttgc caccggccag gttggtgaca tcgaagcgcc
      361 agttgccgcg ggcggcattg cccggcaggg tgaaacggta gaggtagtaa gccatgtcct
      421 gcggctgcag caacgcctgc tgcacgatct ggccatcggg ctggtggatt tcgatattca
      481 gcggctggtt ctgcaccatg aagccgtcgc cgtcccgcag cagtgcgctg aaatcgatgg
      541 tctcgccggg gcgatacaga tcacgcggtg tgtagacgaa cgcttcctgc ggcaactgcg
      601 gccggttgcc cagatcgaat tccgacaggt ccagcgccgg cccctgcaat tgcagcagcg
      661 caatctgctt gccgctgcgg gccagcagat acgatccggt gttgatgtcg ccattgaagc
      721 gcgcttcacc ttctggcgag gtttcccgtt cctgcaccac attgccctgg gcgtccatga
      781 tcgcgacgga aatgccgtcc agcgccgagc catccgccag cgacgacgcc agcaccacca
      841 attcgcccgg atactggcgc gcatgcaggc caatgtcggt caccacaaaa taggtgacgt
      901 agcggcggta atcgtaatcg ccggcttcat ccatcaccgc aacgtaaaaa cccggctcct
      961 gcagttgggg aatgtcttcc accggaatgg attgcttcac cagcgtattg tccggcggat
     1021 tcagatcgaa gcggccgctg taggccagtg tgccccagct gcgcagctgg tcgtagcgat
     1081 aaaagcggtt ctggtagctt tcgctcaggt ggcgggtgaa atcggcgatg tgtttctggt
     1141 ccagacggtg gaagtcgatg ttcacttcct tcacgttgat ggcggtgatc ggaatgccgt
     1201 tggtgagatg ggccggcagc acactgcctt cgctggcaaa accaacggcg ggacgcacat
     1261 cgcgggtggt cagctggcgg gtgacggatt ctttcagttc agcgccggtg agcgcaggca
     1321 ggcctcggtg caccgtcacc tcatagtcgg tgcgcggcgc actggcaggg aaatacaggg
     1381 tgcggcggtc atcggcgaga atccaggcac cgtccacggt tgcgccctga cgctggctca
     1441 cggccagaaa gcgctggaaa tcgcctttcg gatccagcgg caccgacagg gtcaccgcga
     1501 tggcattctt gccctgccat tcccgctccg acacatccag caccgtcaat tcagcgccgg
     1561 caaaacgttc gcgcaaggtt tccagcgact ccaccggcgc gcccttgccg ccgctggcgg
     1621 cctggtcggt ggactcggag caggctgcaa ggaacagccc cagcagcaca acaagcaaca
     1681 atctgcggat ggtcacggtc atctccctgt gagtggtggg cccggtgcag gcgaaagggt
     1741 tcgcaatact atagtcaatt caccggccgg cattatggcg aattcaggca gcagcacgat
     1801 aaatagtgac gtcgctgcgg cttgcatcca atccacttgc gccttgactc cccccaccca
     1861 ccgactacca tagccgtaat tcccgctacc tgccgtctgc atgaaccgat tccgctcgct
     1921 gttccttctc agtattccgg tcctggcgat gggttgcgtc ttcctggtct atggcctgct
     1981 gtcgctgctg ctgttcaggg atcaactgca catccccttc ttcgaactgg ctgccggcac
     2041 gctgctcgcc ggcattgccg gtctgttcgg catttcccgc aagctggaga acatcagcta
     2101 ccgcgaatcc ctgctgttcg tcacgctgac ctggtccatg ctgggcgcgc tgggcggcct
     2161 gccgtttgtg ctgatcaacg gcctgagttt taccgacgcc accttcgagg ccatctcggg
     2221 cctcaccacg accggtgcca ccaccatcac cggactggat ggcctgccca aaaccctgct
     2281 gatgtaccgc cagttcctgc actggatggg tgggctgggg gtgatcattt tcgtggtggc
     2341 gatcatgccg atgctgaaca tcggcggcat gcggctgtac aaggcggaaa cgccggggcc
     2401 cgtgaagggt gaaaagctgg caccgcgcat ttcccacacg gcgcgttacc tgtggtacgt
     2461 gtatgtggcg atcacgctgg cctgcatagc ggcgtactgg ctggcgggga tgaacctgta
     2521 cgatgcagtg gcccatgcct ttgccaccgt gtccaccggc ggcttttcca cccacgatgc
     2581 cagcatcagc tatttcaaca gcccggccat cgaactggtg accatcgtgt tcatgctgct
     2641 gggggcgatg aactttggcc tgcacttcat ggtgtacaac agtcgccggc tgtccctgta
     2701 ctggcgcgac gaggagaccc gctggttttt tggcattttg ctgggtgcca tcctgctgat
     2761 agcactcgca ctttggtggg aaggaaaatt cgacgcactg accagcctgc gccacagtgc
     2821 gttccaggtg gcctcattca tcaccagtac cggctacttc agtaccgact acaccgtgtg
     2881 gcccggcgcg gtcatattgt tgctgatcgt cctgcagtac atcggcggct gcgccggctc
     2941 caccgccggt ggcaacaagg tggtgcgcaa tgccatcacc atgaaagcga tttttctgga
     3001 ag
//
LOCUS       DASUEG010000556         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_576247, whole genome shotgun sequence.
ACCESSION   DASUEG010000556 DASUEG010000000
VERSION     DASUEG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576247"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VFX11_10450"
     CDS             complement(<1..1484)
                     /locus_tag="VFX11_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4939086.1"
                     /translation="MIRFLQQRPFSRRSPLSLTPARLRWLLLALWLLLVIPTLVLVLQ
                     ARQQIKWEALYQHRTLAEEFSQRIDERLQQWIALEESRSPADYQFLVPGSEQLSTLAQ
                     WPVPATVPGLIGYFQVDEQGFFSTPLLPANAAESRQFRQPLLNASEREAVSQQLQDVL
                     GANRLVEKPRDSRRSGLDMAGTIAPAPQAPLQEYPLQESAAASTATHPESSDLESKSQ
                     ALAVTELPYAQQNFDRLRASSSRQQKVLGRIDELQLDDQFANADAKDKGMEVEMKAKA
                     AQEQLEKDKSLKKESHAAPDTPVAQTFALGDAAKPSPAPAATVRLFENEVDPFEFALL
                     DSGHFVLFRKVWQNGGRRIQGVLLDQSAFFDGVLRDSFQPTALAQMSNLILAFQGDVL
                     RVVDGSDAATGSADTTLTSARDIRGTLLLQQKLSAPLGDLQLIFSLNRLPDGPGARVV
                     TWTSLLLGFLLLAVFYALYRLGLRQIRLTRQQQDFVSAVSHELKT"
     gene            complement(1481..2194)
                     /locus_tag="VFX11_10455"
     CDS             complement(1481..2194)
                     /locus_tag="VFX11_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4939087.1"
                     /translation="MQRKLRLLIVEDETAIRTGLTDVFVFHGYDVDVAVDGNEGLRKA
                     GSGQFDLILLDVMLPGLDGFAVCEAIRSRDRDQPIIMLTARNTDDDIVRGLALGADDY
                     VAKPFSVAQLVLRVQAVLRRTRNHIEQLPRLILAADLHIDTANLSGNIRGTTVAFTRR
                     EMEILQYLHAHRERPVPREELLHRIWGYARDLDIETRTVDIHIAKLRRKLEADAKNPV
                     HLVTVRGGGYRLLTQQDPA"
BASE COUNT          420 a          734 c          717 g          455 t
ORIGIN      
        1 gttttcagtt catggctgac ggcggacaca aaatcctgtt gctggcgcgt gaggcggatc
       61 tggcgcaggc ccagccggta gagggcgtaa aacaccgcca gcagcagaaa accgagcaac
      121 aggctggtcc aggtaacgac acgggcgccc ggcccatccg gcaggcggtt caggctgaaa
      181 atcagttgca gatcccccag cggcgccgac aatttctgtt gcagcagcag ggtgccgcga
      241 atatccctcg ccgaggtcag cgtggtgtcg gcgcttcctg tcgcggcgtc gctgccatcc
      301 accacccgca acacatcgcc ctggaaagcc aggatcagat tgctcatctg ggccagtgcg
      361 gtgggctgga aactgtcgcg cagcacgccg tcaaaaaacg cggactgatc cagcagcacg
      421 ccctggatgc ggcggccgcc gttttgccac actttacgga acagcacgaa gtgaccgctg
      481 tcgagcagcg cgaattcgaa cgggtccact tcattttcaa acaggcgcac ggtagcggcg
      541 ggcgccggtg acggttttgc ggcatcgccc agcgcaaatg tctgcgccac aggcgtgtcc
      601 ggcgcagcat gactttcctt cttgaggctc ttgtccttct cgagctgctc ctgcgctgcc
      661 ttggccttca tctccacctc catgcccttg tccttcgcgt cggcattggc gaattggtca
      721 tccagttgca actcatcgat gcggcccagc actttttgct ggcggctgct gctggcccgc
      781 aggcgatcga aattctgctg cgcgtacggc aattcagtga cagccagcgc ctggcttttc
      841 gattcgaggt cgctggattc ggggtgggtt gctgtcgatg ccgcagcgga ttcctgcaaa
      901 gggtattcct gcaatggggc ctggggcgcc ggtgcaatcg tgccggccat gtccagtccg
      961 ctgcgcctgc tgtcgcgggg cttctccacc agccggttcg cgcccagcac gtcctgcaat
     1021 tgctggctca ccgcttcccg ttcgctggcg ttcagcagtg gctgcctgaa ctggcgcgat
     1081 tccgccgcgt tggccggcag caacggcgtg ctgaaaaagc cttgttcgtc cacctgaaaa
     1141 taaccgatca gcccgggcac cgttgcaggc acgggccatt gtgccagcgt cgacaattgt
     1201 tccgagccgg gcaccagaaa ctggtagtcc gccgggctgc gggattcctc cagtgcgatc
     1261 cactgctgca ggcgctcgtc gatgcgctga ctgaattcct ctgccagcgt gcggtgctgg
     1321 tacagcgctt cccatttgat ttgctggcgt gcctgcagca ccagtaccag ggtgggaatg
     1381 accagcagca gccacagcgc cagcagcagc cagcgcaggc gtgccggcgt gagcgacagg
     1441 ggtgagcggc gcgaaaatgg gcgttgctgc aaaaagcgga tcatgcaggg tcctgctgtg
     1501 tcagcagacg gtagccacca ccgcgcacgg tcaccagatg cacgggattt ttcgcgtccg
     1561 cttccagctt gcggcgcagc ttggcaatgt gaatgtccac ggtgcgggtt tcaatgtcca
     1621 gatcgcgggc gtaaccccag atccggtgca gcagttcctc ccgtggcacc ggccgttcgc
     1681 ggtgtgcgtg caggtattgc aggatttcca tttcacgacg ggtgaatgcc acagtggtgc
     1741 cacggatgtt gccgctcagg ttggcggtgt caatgtgcag gtctgccgcc agtatcagcc
     1801 gcggcagttg ctcgatatgg ttgcgcgtgc gtcgcaacac cgcctgcacc cgcagcacca
     1861 gctgtgccac cgagaacggc ttggcaacat agtcatccgc gcccagcgcc agtccgcgca
     1921 cgatgtcgtc gtcggtgttg cgcgcggtga gcatgatgat gggctggtcg cgatcgcggg
     1981 aacgaatggc ttcgcagacg gcaaaaccat cgaggcccgg cagcatcaca tccagcagga
     2041 tcagatcgaa ttgccctgaa ccggccttgc gcagcccctc attgccatcc acggcaacat
     2101 ccacgtcgta gccatgaaaa acaaacacat ccgtcagacc ggtgcgaatg gcggtttcat
     2161 cttccacgat cagcaggcgt agcttgcgtt gcacgggtgg tcctcgggtg acggcagtac
     2221 gtctgatgct agcaagcccc cgctcacccc gcacgtaaac ttttggtaaa aagcgccgca
     2281 gcggttttac caaactgtgc ctgccgctgc cagccgggcc tcccta
//
LOCUS       DASUEG010000557         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1195293, whole genome shotgun sequence.
ACCESSION   DASUEG010000557 DASUEG010000000
VERSION     DASUEG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195293"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VFX11_10460"
     CDS             <1..1279
                     /locus_tag="VFX11_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939088.1"
                     /translation="RLKALIDLGLEQAAEQDPQRLLASFCAAARKIIGARYAVVAVTD
                     QASRTLHYRFTAGMDASVANAVSEAVLSLTPERISSQHLQIASGDAQMAGLPPSHPPV
                     HDVIREPIASLGQVFGCLFLANKLGEARFSAEDRGLARVMTAQLARIYANASLYAQVQ
                     RQMSNLETEIYERQRAQEEVQQLNAELEQRIAIRTAALKEANAELEAFSQTVAHDLQT
                     PLRAVNGFAKLLNDQYGASLPPEGKRFLDVVSSNARSMHQLLENMLAFARFAQQSLNV
                     RPLDMTTLAHQACNDVHEHYRARELDVHIDALPSAPGDPLLLRQVWHNLLDNACKFSG
                     PQDPIRIEVGGRQRSTECLYWVRDFGQGFASDQAGQLFSSFRRGSSMASDGVGLSVCK
                     RIIQRHGGSIWADGAPGAGATFWFTLPRTAGGE"
     gene            complement(1366..>2682)
                     /locus_tag="VFX11_10465"
     CDS             complement(1366..>2682)
                     /locus_tag="VFX11_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4939089.1"
                     /translation="INSKYRTLKGRNYTALAGSSMGGYMTIYTGMAYPKVFGKLASFS
                     TVALDNPMQGQQLRAFVEAARTDPARSPYIAGTQIYLDMGDAEQLSYTTSPLLVENHR
                     QMCNSLLKAGFYPICRVIPGAAHNEAAWSLRVDDAFRFLNYKSGVFSKTWKQVYVRGT
                     HNGFAAGTSMKLVANDTWETTVTFGAGSNERFKFDLNGDWQNTLGDSNKDGVAEAGGA
                     DILVTQGAGSYRIRIHEQMLSYSVVRVDGNQAPLAAAGPDQSINSTTSVSVTLDGSAS
                     SDADGSISSWLWAEWINGAWQAVGSSARLTLAAVTPGEHRYRLTVTDNQGASATDEVL
                     VRVGASWTQLYPQVYVRGTHNNWGLTTTMQLVANNTWEVVATFGAATTERFKFDVYGD
                     WKTNFGDTNKDGTAEAGGGDIPVSGGAGSYLIRFNDQTRVYSVVKQ"
BASE COUNT          510 a          879 c          792 g          501 t
ORIGIN      
        1 gcgcctgaaa gcactgatcg acctgggcct ggaacaggcg gcggaacagg acccgcagcg
       61 cctgctggcc agtttctgtg ccgcagcgcg caagatcatc ggtgcccgct atgcagtggt
      121 ggcggtgacg gatcaggcca gtcgtaccct gcattaccgc tttaccgccg gcatggatgc
      181 cagtgtagcc aacgcggtca gtgaagccgt gctcagcctg acccccgaac gcatcagctc
      241 acaacacctg cagattgcca gcggggacgc acaaatggcc ggactgcccc ccagccatcc
      301 gccagtacac gatgtgatcc gcgagcccat cgccagcctg gggcaggtat ttggctgcct
      361 gtttcttgcc aacaagctgg gcgaggcgcg tttttctgcc gaagaccgcg ggctggcgcg
      421 ggtgatgacc gcccagctgg cacgcattta cgccaatgcc agcctgtacg cccaggtgca
      481 gcgacagatg agcaatctgg aaaccgagat ctacgagcgc cagcgcgctc aggaagaagt
      541 gcagcagctg aacgccgaac tggaacagcg cattgccatc cgcactgcgg cactcaagga
      601 agccaatgcc gaactggaag cgttttccca gaccgtggct cacgacctgc agaccccgct
      661 gcgggcggta aatggcttcg ccaaactgct gaacgaccaa tatggcgcca gcctgccgcc
      721 tgagggcaaa cgctttctgg acgtcgtgtc cagcaatgcc cgctccatgc accagttact
      781 ggagaacatg ctggcatttg cccgttttgc ccagcagtcg cttaatgtac gcccgctgga
      841 catgaccacc ctcgcccatc aggcatgcaa cgacgtgcac gaacattacc gcgcgcgcga
      901 actggacgta catattgatg cactgccctc cgcccccggc gaccccctgc tgctgcgtca
      961 ggtatggcac aacctgctgg ataatgcctg caagttctcc gggccgcagg accccatccg
     1021 catcgaagtg ggcgggcgcc agcgcagcac cgaatgcctg tactgggtcc gggatttcgg
     1081 ccagggcttt gcgtccgacc aggccggcca gctgttctcc agcttccggc gcggcagcag
     1141 catggccagt gatggcgtcg gcctgtccgt gtgcaaacgc attatccagc gccacggcgg
     1201 cagcatctgg gccgatggcg ctcccggtgc cggcgccacg ttctggttta ccctgccgcg
     1261 cacagccgga ggcgaataag tggcatgagc gccccgctcc ggcgcaaccc gtgcagggcg
     1321 cgatgagcaa agcgcattgc atcaccgccc cgtgcgcgcg agtccctact gcttgaccac
     1381 gctgtaaacg cgcgtctgat cgttgaagcg gataaggtag ctgccggcgc cgccgctgac
     1441 cgggatatca ccgccaccgg cttcggccgt gccatccttg ttggtatcac cgaaattggt
     1501 tttccagtcg ccatagacgt caaacttgaa tcgctcggtc gtagcggcac caaaggtggc
     1561 caccacttcc caggtattgt tggccaccag ctgcatggtg gtggtcagac cccagttatt
     1621 gtgggtaccg cgcacataca cctgcgggta cagctgcgtc cagctggctc ctacacgcac
     1681 cagcacttca tccgtggcgc tggcgccctg attgtcggtc acggtcaggc gatagcggtg
     1741 ttcgcccggg gtaacagcgg ccagtgtcag ccgcgcactg ctgccgactg cctgccaggc
     1801 gccgttgatc cattcggccc acagccagct gctgatgctg ccatcggcat cggacgaggc
     1861 gctgccgtcc agcgtgacac tgacactggt cgtgctgttg atggactgat ccgggcctgc
     1921 ggccgccagc ggcgcctggt tgccgtccac ccgcaccacg ctgtaactga gcatctgctc
     1981 gtgaatgcgg atgcgatagc tgcccgcccc ctgcgtcacc agaatgtcag caccgccagc
     2041 ttccgccacg ccatccttgt tgctgtcacc caaggtgttc tgccagtcgc cattcagatc
     2101 aaacttgaag cgttcattgc tgcctgcccc aaaggtgacc gtggtttccc acgtatcgtt
     2161 agccaccagt ttcatcgacg tgccggcggc aaacccattg tgcgtaccgc gcacatacac
     2221 ctgcttccag gttttgctga agacaccgct cttgtaattg agaaagcgga aggcatcatc
     2281 cacgcgcagg gaccaggccg cctcgttatg ggccgcgccg ggaatgacac ggcaaatcgg
     2341 ataaaaaccg gccttgagca gggaattgca catctgcctg tggttttcca ccagcaaggg
     2401 cgaggtggta tagctcagtt gctcggcatc gcccatatcc agataaatct gcgtgccggc
     2461 aatatacgga ctgcgtgccg gatccgtgcg cgccgcttcg acgaaggcgc gcagctgctg
     2521 gccctgcatc gggttatcca gagcaacggt ggaaaacgac gccagcttgc cgaatacctt
     2581 gggataggcc atgccggtat agatcgtcat gtagccgccc atggaactgc cagccagcgc
     2641 cgtgtaattg cgccccttga gcgtgcggta tttgctgttg at
//
LOCUS       DASUEG010000558         4762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1528543, whole genome shotgun sequence.
ACCESSION   DASUEG010000558 DASUEG010000000
VERSION     DASUEG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528543"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFX11_10470"
     CDS             complement(<1..1028)
                     /locus_tag="VFX11_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4939090.1"
                     /translation="MSFDTLGLAEPLLRAVREKGYVTPSPIQAQAIPAILQGQDIMAA
                     AQTGTGKTAGFTLPLLQRLMDGPAVKPNGVRALVLTPTRELAAQVAESVETYGKFLKL
                     RSTVVVGGVSINPQMKNLRRGVDVLVATPGRLMDLYQQNAVKFNQIEILVLDEADRML
                     DMGFIHDIKKILKLLPENRQNLLFSATFSGEIRTLAKGLLNSPVEIDVAPRNTTASLV
                     EQLVHPVDKAKKAALLSHLIRSQEWTQAIVFSRTKHGANRLVEYLMGDGISAAAIHSN
                     KSQGARTKALSEFKQGKVSILVATDIAARGLDIPQMPQVVNFDLPNVPEDYVHRIGRT
                     GRAGEAGHA"
     gene            1380..2987
                     /locus_tag="VFX11_10475"
     CDS             1380..2987
                     /locus_tag="VFX11_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEX4939091.1"
                     /translation="MISTANITMQFGAKPLFENVSVKFGNGNRYGLIGANGCGKSTFM
                     KILGGELEPSAGNVSIDMNERLGKLRQDQFAYEEFTVVDTVIMGHAELWKVKEERDRI
                     YSMADMSEEDGIRVAELEVEFGEMDGYTAESRAGELLLGLGIPLEQHSGLMSAVAPGW
                     KLRVLLAQALFSDPDILLLDEPTNHLDINTIRWLEDILKDRTSTMIIISHDRHFLNSV
                     CTHMADLDYGEIRIYPGNYDAFMTASTMAREQMQADNEKKLAKIAELQTFVSRFSANA
                     SKAKQATSRAKQIDKIELTEIKPSSRVSPFIRFDQNKKLHRQALELIDLNQGYGDNVL
                     IKRFSLLVEAGERVAIIGPNGIGKTTLLRTMIGEMAPQSGEVKWAENAEIGYYGQDHG
                     DDFLQELTLFQWMMQWKPERGDEQLIRGTLGRMLFGQDDIGKKVQSLSGGEQGRMLFG
                     KLILQRPNVLVMDEPTNHLDMESIEALNLALENYPGTLIFVSHDREFVSSVATRIIEM
                     QPGGRIVDFSGSYEEYLRSQGVYQVAK"
     gene            3101..4462
                     /locus_tag="VFX11_10480"
     CDS             3101..4462
                     /locus_tag="VFX11_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX4939092.1"
                     /translation="MLLRQSPASVLPSFRATFFPPLKTLGTALLLCSLAACASQETVA
                     PAPVEAPAPAPAVVPDAAPDAGPASSETRHPPLPQPDFATCIPQLQQRARNAGISVAT
                     VEQVLGQVQYVPRVIELDRKQPEFSETFRNYLTLRVTDQRIEQGRKLLSQHRALLQQL
                     ERDYGVPPHYLLAFWGLETNYGSFLGKMPVLDSLATLACDPRRSEYFTLELLEALQLV
                     DSGTVTPEKFIGSWAGAVGNMQFMPTNYRRYAVDADSSGKPDLWRSIPDAMTSAANYL
                     NQIGWQKNRRWGREVLLPANFDYAQANLQQHKSLQEWRMLGVTLPDGKPLPAQDLQGA
                     ILVPSGHTGPAFIVYDNFDVIMKWNRSEFYAIAVGHLADRIKGEGPLQRTPPDLPRLT
                     LEQVKALQSALNAAGYAAGVADGVLGSGTKKSLRAYQRANGMIADGYPAPDVFKALGV
                     VYP"
     gene            4474..4716
                     /locus_tag="VFX11_10485"
     CDS             4474..4716
                     /locus_tag="VFX11_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939093.1"
                     /translation="MQSMAVSVRVFIRLMLLPLVLVMLLVAGCSSTPEDTNDTSKPVD
                     VVPGCEDATNPQDCRIQHRKGGSDVPTVDNVLETKP"
BASE COUNT          973 a         1345 c         1467 g          977 t
ORIGIN      
        1 gcgtggccag cttcaccggc acgaccggtg cggccgatgc ggtgcacata atcttccggc
       61 acgttgggca aatcgaaatt caccacctgc ggcatttgcg gaatgtcgag accgcgcgcg
      121 gcgatatcgg tggccaccag aatcgacacc ttgccctgct tgaattcact cagcgccttg
      181 gtgcgggcgc cctggctctt gttactgtga atcgcggcag cggaaatgcc gtcgcccatc
      241 aggtattcca ccagacggtt ggcgccatgc ttggtgcggc tgaacacaat tgcctgggtc
      301 cattcctgcg accggatcag atggctcagc agtgcggctt tcttggcttt atcaaccgga
      361 tgcaccagct gctccaccag cgaggccgtg gtattgcgcg gtgccacatc gatttccacc
      421 gggctgttca gcaggccctt ggccagagtg cggatctcgc cggaaaaggt ggcggagaac
      481 agcaagttct ggcgattctc gggcagcagt ttcaggattt tcttgatgtc gtggatgaag
      541 cccatgtcca gcatgcggtc ggcttcgtcc agcaccagaa tttcgatctg gttgaatttg
      601 acggcgttct gctggtacag gtccatcaga cggcccggtg ttgccaccag cacatcgacg
      661 ccacggcgca gatttttcat ctgcgggtta atgctgacac caccaactac gacagtggag
      721 cgcagcttga ggaatttgcc ataggtttca acgctttcag ccacctgggc ggccagttcg
      781 cgggtcggtg tcagcaccag cgcgcggacg ccgttgggtt tgacagcggg gccgtccatc
      841 agccgctgca acagcggcag ggtgaagcct gcagtcttgc cggtgccggt ctgggcagcg
      901 gccatgatgt cctggccttg caaaatggcg gggatcgcct gggcctggat cggggagggc
      961 gtgacgtagc ctttttcgcg cacggcacgc agtaacggtt cagctaaacc gagggtatcg
     1021 aatgacatgt agtgttctct ggttcgcaat gaaattgctt gataagttaa gggcttgcgc
     1081 tgaagtggca agcgttgcgc ggcatgcgga aagtggcgct cagaagggat atcgaggcag
     1141 gctgcaatgg gaatggtgca accttgacaa ctaacgatga cgcaggacag ccgggtatta
     1201 aaccgcgagc tgcgaatgca agtgtaacac atcagggggg tttccaggct atttttgttg
     1261 gtgaggcgat gtcggtgcac gtggtggttg cgctctgtaa cccaaccttt atggttctgc
     1321 cggccggctt ttgctatagt gcgcggcctt gaaaagcccg ttgttgcagg acctcttccg
     1381 tgatttccac cgccaatatc accatgcagt tcggggccaa gcccctgttc gaaaacgtct
     1441 ccgtcaaatt cggcaatggc aaccgttacg gtctgatcgg cgccaacggc tgcggcaaat
     1501 ccacgttcat gaaaatcctg ggcggtgagc tggaacccag cgcaggcaat gtctccatcg
     1561 acatgaacga gcgtctgggc aagttgcgcc aggatcagtt tgcctatgag gaattcacgg
     1621 tggtggatac ggtgatcatg ggccacgccg aactgtggaa agtcaaggaa gagcgcgacc
     1681 gcatttattc aatggcggac atgtcggaag aagacggcat ccgcgtggcg gaactggaag
     1741 tggaatttgg cgagatggat ggctacaccg ccgaatcgcg cgccggtgaa ctgttgctgg
     1801 gtctgggtat tccgctggag cagcattccg gcctcatgag cgcggtggca ccgggctgga
     1861 aactgcgtgt cctgctggcg caggcgctgt tttccgatcc cgatattctg ttattggacg
     1921 aaccgaccaa ccacctggat atcaacacca tccgctggct ggaagatatt ctgaaagacc
     1981 gcaccagcac catgatcatc atttcccacg accgtcactt tctcaacagc gtgtgcacgc
     2041 acatggcgga tctggactac ggcgaaatca ggatttaccc cggcaactat gatgcgttca
     2101 tgacggcctc caccatggcc cgtgaacaga tgcaggcgga caatgaaaag aaactggcga
     2161 agattgccga gctgcagacc ttcgtcagcc gtttttcggc gaacgcatcg aaagccaagc
     2221 aggccacgtc ccgggccaag cagatcgaca aaatcgagct caccgaaatc aaaccgtcga
     2281 gccgcgtcag cccattcatc cggttcgacc agaacaagaa gctgcatcgc caggcgctgg
     2341 aactgatcga cctgaaccag ggctatggtg acaacgtact gatcaagcgt ttcagcctgc
     2401 tggtggaagc gggtgagcgt gttgccatca tcggccccaa cgggatcggc aagaccacgc
     2461 tgctgcgcac catgatcggt gaaatggcgc cgcagtccgg tgaagtgaaa tgggccgaga
     2521 atgccgagat tggctactat ggccaggatc acggcgacga ctttctgcag gaactcactc
     2581 tgttccagtg gatgatgcag tggaagccgg agcgcggcga tgagcagctg attcgtggca
     2641 ccctggggcg gatgctgttc gggcaggacg atatcggcaa gaaggtgcag tcgctgtccg
     2701 gcggcgaaca gggccgcatg ctgttcggca agctgatcct gcaacggccc aacgtgctgg
     2761 tgatggacga acccaccaac cacctggaca tggaatccat cgaggcactg aacctggcgc
     2821 tggaaaatta tcccggaacg ctgatcttcg tcagccacga ccgcgaattc gtgtcgtcgg
     2881 tggccacgcg catcatcgaa atgcagccag gtggcaggat cgttgatttc agcggcagct
     2941 acgaagaata tctgcgtagc cagggtgttt atcaggtggc gaagtagggc gtgcaccctg
     3001 ccttcgccat cccgcacttt tacttccccg cgcgataagg taaaaaagca gcatcccggc
     3061 ggtggctcag gcgaccgcct cattcctgta aaaggacacc atgttgctgc gtcaatcccc
     3121 tgcttctgtt cttcccagtt ttcgggcgac gttttttccg ccgctgaaaa ccctcggtac
     3181 ggcattgttg ctgtgcagtc tggccgcctg tgcgtcgcag gaaaccgttg ctcctgcgcc
     3241 ggtggaggcg cccgcacctg cgccggctgt ggttccggat gcggcacctg atgccggacc
     3301 ggcatccagc gagacccgcc acccaccgct gccgcagccg gattttgcca cctgcattcc
     3361 acagttgcag cagcgcgcgc ggaacgccgg catatcggtt gccaccgtgg agcaggtgct
     3421 gggccaggtg cagtatgtgc cgcgggtgat cgagctggat cgcaagcagc cggaattcag
     3481 cgagactttc cgcaactacc tgacgctgcg cgtcaccgac cagcgcattg agcaaggccg
     3541 caaactgctg tcgcagcatc gtgcgctgct gcagcaactg gaacgcgatt acggcgtgcc
     3601 gccgcactat ctgctggcgt tctgggggct ggaaaccaac tacggcagtt ttctgggcaa
     3661 gatgccggtg ctggattcac tggccaccct ggcctgcgat cctcgccgca gcgaatactt
     3721 caccctggaa ttgctggagg ccctgcagct ggtggacagc ggcaccgtga cgccggaaaa
     3781 attcatcggc tcctgggccg gcgccgtggg caacatgcag ttcatgccca ccaattaccg
     3841 ccgttacgcg gtggatgccg acagcagtgg caagcccgat ttgtggcgca gcattcccga
     3901 tgccatgacg tcggccgcga attatctgaa ccagatcggc tggcagaaaa accggcgctg
     3961 gggccgcgaa gtgttgctgc cggcaaattt cgattacgcg caggcgaatt tgcagcaaca
     4021 caaatcgctg caggaatggc gcatgctcgg tgtcacgctt cccgatggta agccgttgcc
     4081 ggcgcaggat ttacagggcg cgatcctggt gcccagtgga cacaccggtc ccgcgttcat
     4141 cgtgtacgac aatttcgatg tgatcatgaa atggaatcgc tctgagtttt atgccatcgc
     4201 ggtggggcac cttgccgatc gcatcaaggg tgaaggcccg ctgcaacgca cgccaccgga
     4261 cctgccgcga ctgacgctgg aacaggtgaa ggcgctgcag tctgcgttga atgcagccgg
     4321 ttatgctgcc ggcgtggcgg acggtgtgct gggctccggc accaaaaaat ccctgcgggc
     4381 ctatcagcgc gcaaacggca tgattgccga tggttatccg gccccggacg tgttcaaggc
     4441 gctgggtgtg gtttatccgt gaggaaatcg agtgtgcaat ccatggcagt gagtgttcgt
     4501 gtttttatcc ggttgatgct gttgccattg gtgctggtga tgctgctggt ggcgggctgc
     4561 tcatcgacgc cggaggatac gaacgatacc tcgaagccag tggacgttgt tcccggttgt
     4621 gaagatgcga caaatccgca ggactgtcgc atacagcacc ggaagggtgg cagcgacgtg
     4681 cctactgtcg acaatgtgct ggaaactaaa ccctgacacg ctggaatact gcagctctta
     4741 agtgtccagt ttcatcttgc gc
//
LOCUS       DASUEG010000559         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_886088, whole genome shotgun sequence.
ACCESSION   DASUEG010000559 DASUEG010000000
VERSION     DASUEG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886088"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFX11_10490"
     CDS             complement(<1..113)
                     /locus_tag="VFX11_10490"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX4939094.1"
                     /translation="MTVAVWFRSDLRVQDNPALHAACSSREPVLAFYFFTP"
     gene            complement(110..1072)
                     /locus_tag="VFX11_10495"
     CDS             complement(110..1072)
                     /locus_tag="VFX11_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HEX4939095.1"
                     /translation="MHPKAPVAISRCLLGEQVRYNGDHKHNRFCTGVLAEYFEFVPVC
                     PEIQAGMSVPRKPIRLVLEEDEQIRARESDDASADHTVALEQQAHLFVQDNPAIAGFI
                     ATPNSPSCGLFTVKLYLPNGNPVNKTSGLFSKTLRQLNPLLPVEESGRLNDPGLRENF
                     IMRVYTYHRWQELNRQPLTANGLVQFHTRHKYLVMSHSHEAYKNLGQMVARAGNSDLT
                     QLGPDYIRALMDAIAAPAPRVRHCNVLHHLLGYLKKMINSNDKQELLATIEHYRQGIV
                     PLIVPMALLRHHFERWRGQQRYVLEQVYLDPYPFELGLRSQVHP"
     gene            1828..>2244
                     /locus_tag="VFX11_10500"
     CDS             1828..>2244
                     /locus_tag="VFX11_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939096.1"
                     /translation="MLMVLLVCLPAAARDNRAESRYYGSQDDDYPYPQRSDSRSNSRS
                     VDRQATTGRGQSAHVRRDQDGGRQSSRDGGYYDSRGADRSRSRRDDSRNSRDDRRVDY
                     RSDERDSRSYNSRTSNTRSHDERYEQRRSGRDDERYD"
BASE COUNT          442 a          630 c          692 g          480 t
ORIGIN      
        1 tccggcgtga aaaaataaaa cgccagcacg ggctcacggc tgctgcaggc agcgtgcagg
       61 gccggattgt cctgcacccg caggtcgctg cgaaaccaga ccgcgactgt catgggtgca
      121 cctgactgcg caggcccagc tcaaacggat acggatcgag atacacctgc tccagcacat
      181 agcgctgctg gccgcgccag cgttcgaagt gatgccgcag cagcgccatt ggcactatca
      241 acggcacgat gccctgacgg taatgctcga tggtggccag cagttcctgc ttgtcgttgc
      301 tgttgatcat ttttttcagg tagcccagca ggtgatgcag cacgttgcag tgacggacgc
      361 ggggtgccgg cgcggcgatg gcatccatca gcgcgcggat atagtcgggg cccagctgcg
      421 tcagatcgct gttgccggca cgggccacca tctgccccag gtttttgtac gcctcgtggc
      481 tgtggctcat caccagatat ttgtggcggg tatggaattg caccagaccg tttgccgtca
      541 gcggctggcg attcagttcc tgccagcggt gataggtgta aacacgcatg atgaaatttt
      601 cccgcaggcc cggatcgttc aggcggccgg attcctccac cggcagcagc ggattcagct
      661 ggcgcagggt cttgctgaac aggccgctgg tcttgttcac cgggttgccg ttgggcagat
      721 acagcttcac cgtgaacagg ccgcagctgg gggaattggg ggttgcaata aagccggcaa
      781 tggcagggtt gtcctggaca aaaagatggg cttgctgttc aagggcgacg gtgtggtctg
      841 ccgatgcgtc gtctgactcc cgggcgcgaa tctgctcgtc ctcctccagt accagccgga
      901 tcggcttgcg cggcaccgac attcctgcct gaatttccgg gcaaactggc acgaattcga
      961 agtattctgc caacacgcct gtgcagaagc ggttgtgctt gtgatcaccg ttgtagcgga
     1021 cctgttcgcc cagaaggcag cggctgatgg ccacgggagc tttcggatgc atgattattg
     1081 tccatgtaat gtcctagaca aaaccaatcc tacgccggat gttcacccct gtcaaaccgg
     1141 caggcatgca catccggtct caaaggtttg atagagcgac ctgtgggggg gagcgggata
     1201 gtgcgggagg ctgttaggcc gaggcgtaag ggcagggcat ataaacgaaa aagccggcgg
     1261 gaggccggct gtctgcttgc tgatggttcc agtaagcggt ttggggaggc gtggtggtcg
     1321 ctatccgaaa tacatgttct caagatattc tttcagggcg gcaaacgaaa atgcctgctc
     1381 cggatgggtg atctcgaaca acaggttggc cgattccttg atagtcatgg tgtgcctcca
     1441 gagtgttctg ataaacagag tcctgatggg taaacgggtc gttttgccca ggggcggtga
     1501 ttccagcccc ctgcacctgt atcgaagcat agcctgtgcc ataattgccc tgcgcggcta
     1561 acctgatgaa ttggcgtgtt tttccattga cgtcgcagat ctcgcatacc ggtgctcaca
     1621 ctgaacagca tcatgtgcgg gcaccgaatt cagagaatgt tcagatagcg tgcgcaaaaa
     1681 tgtcctgtat tcgcgggaag tcgggcattc tgtgaatcgg ttgtcgtcga tgctgctgtt
     1741 gccctttgtt gatatccagt tgaggctccg ttgaaggaac tatttccgtc cctgcaggct
     1801 atccggcgct ccgcatggtg ggtgagcatg ctgatggtgt tgctggtctg cctgccggcg
     1861 gctgcgcgcg acaatcgtgc agaatcccgt tattacggtt cgcaggacga cgattaccct
     1921 tacccgcaac gcagtgacag ccgcagcaac tcgcgcagcg tggaccggca ggccactact
     1981 ggccgcggcc aatccgccca tgtgcgccgt gatcaggacg gcggtcgcca gtcttcccgt
     2041 gatggcggct actacgactc ccgcggtgcg gaccgcagcc gttcccggcg cgacgattcc
     2101 cgcaattccc gagacgatcg ccgagtcgat taccgcagcg atgaacgcga ctcccgctcc
     2161 tataattcgc gcacgtccaa tacccggtcc catgatgaac ggtacgaaca acgccgcagc
     2221 ggacgcgacg atgaacgtta tgac
//
LOCUS       DASUEG010000560         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2175204, whole genome shotgun sequence.
ACCESSION   DASUEG010000560 DASUEG010000000
VERSION     DASUEG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175204"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFX11_10505"
     CDS             <1..436
                     /locus_tag="VFX11_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939097.1"
                     /translation="GLLASSVALAEDKVYMLGKLKVKDSTYVSVVFFQDPAVNTIAIC
                     EREVMYGRNGQWQYFAHLVHKVQGVSLTVDYFCRSTANPVAVWQPRDPYDYVYLVDIR
                     NDGLKMTEFDTYADCVGSLKKNRTPETFDYFCAKSNQHISKS"
     gene            complement(488..724)
                     /locus_tag="VFX11_10510"
     CDS             complement(488..724)
                     /locus_tag="VFX11_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4939098.1"
                     /translation="MKSKAVFYHAGCPVCVDAEQKLAHALNPGQYQVEIVHLGESKSR
                     LPEAVRLGVKSVPALAIDGDVFHINFGAPIEALQ"
     gene            888..1199
                     /locus_tag="VFX11_10515"
     CDS             888..1199
                     /locus_tag="VFX11_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4939099.1"
                     /translation="MQRSRSEYTRLEDVIGCKWSVSVLLAVGQGVCRPGALERHIEGI
                     STKVLSERLRKLSAYGLLEKQVYPEVPPRTEYRLTALGIRLAEIISQIQQLDPRSASP
                     R"
     gene            1382..3043
                     /gene="sulP"
                     /locus_tag="VFX11_10520"
     CDS             1382..3043
                     /gene="sulP"
                     /locus_tag="VFX11_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX4939100.1"
                     /translation="MLRHYRRAWFRQDLFAGLVLVSMLVPVGIAYAEASGVPGIYGLY
                     ATIVPLLAYALFGPSRILVLGPDSALAALILSVVLPLSGGDPMKALTLASMMAVVAGL
                     VCIVAGLLRLGFITELLSKPIRYGYMNGIAFTVLISQMPKLFGVSIESAGPLRDIGQL
                     CLLVFTGQFNVYTFAVGGGTLAIILLFKRFEKVPVILFAVVLATLAVQFLDLDGQGVK
                     VLGQLPQGLPSFALPWLSGVDLATVVLGGCAAALVAFADTSVLSRTYAARARVYVDPN
                     QEMVGLGIANLATGFFQGFPISSSSSRTPVAEAAGSKTQVTGIIGALGMAALLLLAPN
                     LMEYLPNSALAAVVIAAALGLFEFTDLRRIFRIQQWEFWLSMTCFAGVAIFGAVPGIC
                     IAVVLAVIEFLWDGWRPHFAVLGRVDGVRGYHDVMRYPHARRVPGLLLFRWDAPLFFA
                     NAELFQQRVLQCIGEAPDRIRRVVVAAEPVTSVDVTSADMLRELAGILHQSGIELHFA
                     EMKDPVKDKLVRFELMEQLGEGIFHPTVGAAVDDYLRDHRVDWIP"
     gene            3182..>3292
                     /locus_tag="VFX11_10525"
     CDS             3182..>3292
                     /locus_tag="VFX11_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939101.1"
                     /translation="MSSVVSEQRVLSGMRPTGSLHLGHYHGVLKNWVKLQH"
BASE COUNT          580 a          893 c         1064 g          755 t
ORIGIN      
        1 gggactgctg gccagcagtg tcgcactggc agaggacaag gtgtacatgc tcggcaagct
       61 gaaagtgaag gacagcacct atgtatcggt ggtgtttttc caggatcctg ccgtgaatac
      121 gattgcgatc tgcgaaaggg aagtcatgta cggacgcaac gggcaatggc agtacttcgc
      181 acacctggtg cacaaggtgc agggagtgtc gctgacggtg gattatttct gccgcagcac
      241 ggccaatccg gttgcggtct ggcagccgag ggatccttat gattacgtct atctggtgga
      301 tatccgcaat gatggcctga aaatgacgga gttcgatacc tatgccgatt gtgtcggtag
      361 cctgaagaag aaccgcacac ccgaaacctt cgattatttc tgcgcgaagt cgaaccagca
      421 catttccaaa tcctgaatcc gaggcaggaa gggcggctgt cccctcctgc cgggagttat
      481 ctttccgtta ttgcagggct tcgatcggag caccgaaatt gatgtgaaac acgtcgccgt
      541 cgatggccag tgccggcacg gatttcacac ccagtctgac cgcttccggc aggcgcgatt
      601 tgctttcgcc caggtgcacg atttcaacct ggtactggcc cgggttcaac gcgtgagcca
      661 gtttctgttc tgcgtcaacg cacacggggc agccagcgtg ataaaaaaca gctttcgatt
      721 tcatacactc ttcctttttg ctaggttgtc ggtcggctag attcttgtgc cggtgctgca
      781 ggcacaggtg tactttacgg atcgggtgcc gctgccaaca gtcggcacat ttcggtgggg
      841 taggcacctt ttggtgtaaa ttggcgaaat ggaaggaata aataaaaatg cagagaagtc
      901 ggtcggaata cacacggctg gaagatgtga tcggatgcaa gtggtcggtg tcggtgttgc
      961 tggcagtggg gcagggcgtt tgccggcccg gtgcgctgga gcggcacatc gagggcatct
     1021 ccaccaaggt gctcagcgag cggttgcgca aactcagcgc ttacggtctg ctggaaaaac
     1081 aggtttatcc cgaagtgccg ccgcgcacgg aataccggct gacagcgctg ggaattcgcc
     1141 tggcggagat catcagccag atccagcagc tggacccgcg gtctgcgtcc ccacgctgag
     1201 cgtgtgcggc cctgtcgtgc aatggatttt tcttcatttc ggccgggccg ggtaattgat
     1261 aacagggaaa cgagtcttgg aatcggaaca gcataaacgc actctcgaca ccattcccaa
     1321 tcccgacctg cccttgccca aagtccagac cggatggcag cgctgggtgc cgggtctggt
     1381 gatgctgcgg cattaccggc gcgcgtggtt ccgacaggat ctgtttgcgg ggctggtgct
     1441 ggtgtcgatg ctggtgccgg tggggattgc gtatgccgag gcatccggtg tgccgggtat
     1501 ttacggtctt tacgccacca tcgtgccgct gctggcctat gcgctgtttg gccccagccg
     1561 cattctggtg ctggggccgg attcggcgct tgccgcgctg atactttcgg tggtgttgcc
     1621 gctgtccggc ggtgatccca tgaaggcgtt gaccctggcc agcatgatgg cggttgtggc
     1681 cgggctggtc tgcattgtgg cgggactgct gaggctggga tttatcacgg agttgctgtc
     1741 caagcctatc cgttacggtt acatgaacgg gatagccttc acggtactga tcagccagat
     1801 gccgaaactg tttggcgtct cgatcgagag tgcaggacct ttgcgggata tcggccaact
     1861 gtgcctgctg gttttcaccg gccagttcaa cgtttatacc tttgcggtgg gcggtggcac
     1921 cctcgcaatc attctgttgt tcaaacgctt cgagaaagtg ccagtgatac tcttcgctgt
     1981 ggtattggcg accctggcgg tgcagtttct ggatctggat gggcaggggg tcaaggtgct
     2041 gggtcagctg ccgcagggtc tgccgtcgtt cgcgctgccc tggctcagcg gtgtggatct
     2101 ggccacggtg gtgctgggtg gctgtgccgc tgcattggtg gcctttgccg ataccagcgt
     2161 gctgtcgcgc acctatgccg cccgcgcccg cgtgtatgtg gacccgaatc aggaaatggt
     2221 cgggctgggc attgccaacc tggcgacggg ctttttccag ggttttccca tcagcagcag
     2281 ttcttcccgc acgccagtgg cggaagccgc gggttccaaa acccaggtca ctggcatcat
     2341 cggcgcattg gggatggcag ccctgctgct attggcgccc aatctgatgg aatacctgcc
     2401 caacagcgcg ctggccgccg tggtgattgc cgcggcgctg ggcctgttcg agtttaccga
     2461 tctgcgtcgc atcttccgca tccagcaatg ggaattctgg ctgtccatga cctgtttcgc
     2521 tggcgtggcg attttcggcg cggtgcccgg tatctgcatt gcggtagtgc tggcggtgat
     2581 cgaattcctg tgggatggct ggcgtccgca cttcgcggtg ctgggccggg tggatggcgt
     2641 gcgtggttac catgacgtga tgcggtatcc ccatgctcgc cgggtgccgg gcctgctgct
     2701 gttccgctgg gatgcgccgc tgttcttcgc caatgccgag ctgttccagc agcgggtgct
     2761 gcagtgcatc ggcgaggcgc ccgaccgcat ccggcgggtg gtggtggccg ccgagcccgt
     2821 gaccagcgtg gatgtcactt ccgctgacat gttgcgggaa ctggcgggca tcctgcacca
     2881 gagtggcatc gagctgcatt ttgcggaaat gaaagacccg gtgaaagaca aactggtgcg
     2941 tttcgagctg atggagcagc tgggtgaggg tattttccat cccacggtgg gggccgcggt
     3001 ggacgattat ctgcgcgatc accgggtgga ctggataccc tgaacaccag ctttagactt
     3061 cgcccccaat gccgctataa tcgacgttct cgcactgggg ccatcacacc ccgccatggc
     3121 cgtgaggcca ggggacctcg ccagtgattg aaccgatttt ttgccaaatc aggagcttac
     3181 cttgagttca gtagtgtcgg agcagcgtgt tctgtcgggc atgcgcccca cgggcagcct
     3241 ccatctgggt cattatcacg gtgtactgaa gaactgggtc aaactgcagc ac
//
LOCUS       DASUEG010000561         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1783918, whole genome shotgun sequence.
ACCESSION   DASUEG010000561 DASUEG010000000
VERSION     DASUEG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783918"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFX11_10530"
     CDS             complement(<1..609)
                     /locus_tag="VFX11_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEX4939102.1"
                     /translation="MDKTILVTGATGAQGGALVPRLLERGFKVRALTRNPAKPAGQAL
                     AAQGAELVAGDLDDEASLQRACAGMYGVFSVQNFWEKGVGYQREIAQGCNLARAAKAA
                     NVQHFVQTSVANCDNAAGVLHFQSKYEIEKFIVQLDLPHTFLREVFFMENFTQPVTLS
                     GAKKASDPHWILPLITGLLDDSTRFHMITVDDIAWFAADVFAH"
     gene            complement(743..1666)
                     /gene="xerC"
                     /locus_tag="VFX11_10535"
     CDS             complement(743..1666)
                     /gene="xerC"
                     /locus_tag="VFX11_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX4939103.1"
                     /translation="MTDPQSASASAIDAAVEDYLNHLQQKRLLSQHTVDAYRHDLEHL
                     KPFCAKNAVADCAHLQPHHIRMLIAERHRKGSQSKSLQRQLASLRGLFRFLMQQKKMQ
                     LNPAVGIRPPKGEKRLPHTLDVDQMSQLLDIRDNDPLSVRDRAILELFYSSGLRLSEL
                     LHLQLQNYDRSDNTVRVLGKGSKERIVPVGSAAQQAIAQWLQIRDQFAPQNDTLFVSQ
                     RGTALHPSTVQKRFRDWGIRQGIDRGLHPHLLRHSFASHLLESSGDLRGVQELLGHAD
                     ISTTQIYTHLDFQHLANVYDAAHPRAKKRED"
     gene            complement(1669..2358)
                     /locus_tag="VFX11_10540"
     CDS             complement(1669..2358)
                     /locus_tag="VFX11_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX4939104.1"
                     /translation="MTDITVGKSKQDKLTAADVIAFLRAHPDFFVNRADLLFDLQLPH
                     GHSNASSLLEKQASVLRTRNTELRHKLNELVNVARDNDRLFNQIRKLGLALLETDTLA
                     DCEATLQRSLLQDFQLDCVSLLLFGTEREPGNIIFTSKHELQAILGDLLRGERITCTT
                     LRRKEMAFLFPGYEQSEGSAALIPLNFRGEIGLLAVGSTDPNHYTSNMDTTFASYIGE
                     ILSRRLYHLLG"
     gene            complement(2355..>2744)
                     /gene="dapF"
                     /locus_tag="VFX11_10545"
     CDS             complement(2355..>2744)
                     /gene="dapF"
                     /locus_tag="VFX11_10545"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX4939105.1"
                     /translation="ETVQMACVSMGNPHGVLQVDNVDTAPVEQLGPLLEAHPHFPNKA
                     NIGFCQVVDRDNLRLRVFERGVGETRACGTGACAAAVANIAAGRVNNKVNVTLPGGTL
                     QIEWRGEGHPVLMTGPATTVYEGTITL"
BASE COUNT          480 a          792 c          898 g          574 t
ORIGIN      
        1 gtgcgcgaac acatccgcag caaaccaggc gatgtcgtcc accgtgatca tgtgaaaacg
       61 cgtgctgtcg tccagcaggc cggtgatcag tggcagaatc cagtgcgggt cggaggcttt
      121 cttggcgcca gacagcgtca ccggctgggt gaagttttcc atgaaaaata cttcacgcag
      181 aaaggtgtgg ggcagatcca gctggacgat gaatttttcg atttcgtatt tgctctggaa
      241 atgcagaacg ccagccgcgt tgtcgcaatt ggcgacggag gtctgcacga aatgctgcac
      301 gttggcggcc ttggcagcgc gggccagatt gcaaccctgc gcgatctcgc gctggtaacc
      361 gacgcccttt tcccagaaat tctgcaccga gaacacgccg tacatgccgg cgcaggcacg
      421 ctgcaatgag gcttcgtcat cgaggtcgcc ggcgaccaat tcagcccctt gtgcggccag
      481 ggcctgccct gctggcttgg ccgggttgcg ggtgagggcc cgcaccttga agccgcgctc
      541 cagcagccgg ggcaccagtg cgccgccctg tgcgccggtg gcaccggtga cgaggattgt
      601 cttgtccatt gaacatgctc caggaagcgg tctgcatcca gcgcagaact cgcgggttcc
      661 ggatcataac aacgaaaggc ggactggaaa aggtcaattt gtttgacggc tgcctggcgt
      721 tcggacgcag gtcggtgctg tgtcagtctt cccgtttctt cgcccgcgga tgggccgcgt
      781 cgtatacatt ggccagatgc tggaaatcca ggtgggtgta aatctgcgtg gtggaaatgt
      841 cggcgtggcc cagcaattcc tgcacgccgc gcaggtcgcc gctggattcc agcaggtggc
      901 tggcgaagga atggcgcagc aaatgcggat gcaggccgcg atcgatgccc tgcctgatgc
      961 cccagtcgcg aaaccgtttc tgcacggtgg agggatgcag cgccgtgccg cgctggctga
     1021 caaacagggt gtcgttttgc ggcgcgaact ggtcgcggat ctgcagccac tgcgcgatcg
     1081 cctgttgcgc cgcgctgccc accggcacaa tacgttcctt gctgcctttg cccagtacgc
     1141 gcaccgtgtt gtcgctgcga tcgtaattct gcagttgcag gtgcagcagt tcgcttaacc
     1201 gcaagcccga agaataaaac agttccagaa tggcgcggtc gcgcacggac agcggatcgt
     1261 tgtcgcggat atccagcagc tggctcatct ggtccacgtc cagcgtgtgg ggcaggcgtt
     1321 tttcaccctt gggcgggcgg atgccgacgg cgggattcag ctgcattttt ttctgctgca
     1381 tcagaaagcg gaacaggccg cgcaggctgg ccagttgccg ttgcagggat ttgctctggc
     1441 tgcccttgcg gtggcgctcg gcgatcagca tgcggatgtg gtgcggttgc aggtgggcgc
     1501 agtcggccac tgcgtttttc gcgcagaagg gtttcaggtg ttccagatcg tggcgatagg
     1561 cgtccaccgt gtgttgcgac agcaggcgct tctgttgcag gtgattcaga taatcctcaa
     1621 ccgctgcatc gatggcgctg gcactggcgg attgcggatc ggtcatcgtt atcccagcag
     1681 atgatacaga cggcggctga gaatttcgcc gatgtagctg gcgaacgtgg tgtccatgtt
     1741 cgaggtgtag tggttgggat cggtgctgcc cacggcgagc aggccgatct cgccgcggaa
     1801 attcaacggg atcagggcgg cggaaccttc ggactgttca tagccgggaa acagaaacgc
     1861 catttccttg cgccgcagcg tagtgcaggt gatgcgttca ccccgcagca gatcccccag
     1921 gatcgcctgc agttcgtgct tgctggtgaa aatgatgttg cccggttcgc gttcggtgcc
     1981 gaacagcaac aggctgacgc agtcgagctg gaaatcctgc agcaggctgc gttgcagcgt
     2041 ggcttcacaa tcggccaggg tatcggtttc cagcagggcc aggcccagct tgcggatctg
     2101 gttgaacagg cggtcgttgt cgcgggccac gttcaccagt tcgttgagtt tgtggcgcag
     2161 ttcagtgttg cgggtgcgca gcacgctggc ctgcttttcc agcagcgagg acgcattgct
     2221 gtggccatgg ggcagttgca gatcgaacag caggtcggcg cggttgacga aaaaatccgg
     2281 atgggcacgc aagaacgcga tgacgtcggc ggcggtgagt ttgtcctgct tggatttgcc
     2341 cacggtgatg tcggtcatag cgtaatggtc ccctcgtata cggttgttgc tggcccggtc
     2401 atcagtaccg gatggccttc gccccgccat tcgatctgca aggtgccgcc gggcagcgtc
     2461 acgttcactt tgttgttcac ccgccctgct gcgatattcg ctaccgccgc cgcgcaggcc
     2521 ccggtaccgc aagcccgggt ctcgcccacg ccgcgctcga atacccgcag gcgcaggttg
     2581 tcgcggtcca ccacctggca gaagccgatg ttggccttgt tgggaaaatg cggatgggct
     2641 tccagcagtg gccccagctg ttccaccggc gccgtatcca cattgtccac ctgcagcacg
     2701 ccgtgggggt tgcccatcga cacgcaggcc atctgcacgg tttc
//
LOCUS       DASUEG010000562         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1550310, whole genome shotgun sequence.
ACCESSION   DASUEG010000562 DASUEG010000000
VERSION     DASUEG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550310"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1171
                     /locus_tag="VFX11_10550"
     CDS             491..1171
                     /locus_tag="VFX11_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEX4939106.1"
                     /translation="MLQQLMLATSLALGWLLLTACTSNPAVVDASATPGASNTPAIPV
                     YVASHGWHTGVILPAAGLNQIFPQLRARFGATPFYEIGWGDARFYPADDITSGLTLQA
                     AFFSQGAVVQIVAVPANPVRYFGNSQVRALCLSPAQLDRLQRYIIASLHTDANGQLLP
                     QQKGLYGNSQFYAGNGRFHLLHTCNTWTAKALRSAGFAVSTLTLRADGVLDALEPVSA
                     VCPAITTE"
     gene            1285..1620
                     /locus_tag="VFX11_10555"
     CDS             1285..1620
                     /locus_tag="VFX11_10555"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEX4939107.1"
                     /translation="MKKQIIYGVLALLGITLTWYHNIQFALEHGSFALDQFIADCFVN
                     HAASSITWDVTIAAIAFLTWSNIESKRLQMKGWIAIPFLTLGVALAFALPLYLLLRER
                     HLARRKAAA"
     gene            complement(1650..1823)
                     /locus_tag="VFX11_10560"
     CDS             complement(1650..1823)
                     /locus_tag="VFX11_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939108.1"
                     /translation="MQPESKKPAKSKSNDQSRKDTSQTIAEQTKAFLKAGGQVTRVKA
                     GVSGKPFIPTKNR"
     gene            complement(1902..3032)
                     /locus_tag="VFX11_10565"
     CDS             complement(1902..3032)
                     /locus_tag="VFX11_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4939109.1"
                     /translation="MSIQYTAVDPDGLLEYSVVFTDRSLNHMSALFQQAMRDISGTLK
                     AVYHAQAVAVVPGGGTYGMEAVARQFAGGKHCLVLRNGWFSYRWSQIFEMGAIPARET
                     VLKARPVMAGSKQPFAPAPIDEVVATIRREKPDLVFAPHVETASGIILPDGYLQSVSA
                     AVHEHGGLFVLDCVASGAIWVDMQQCGIDVLISAPQKGWSASPCAALVMLGEDAARRI
                     ESTRSSSFACDLLKWLQIMRAYENGGHAYHATLPTDALLKLRDTMAESHATGFETLRA
                     QQWELGKQVRALLAEKGFSSVAASGFEAPGVVVSYTDDPLIHNGKKFAEQGLQTAAGV
                     PLMCDEPADFRTFRLGLFGLDKWQDVTAAVNHLRQALAAIRA"
BASE COUNT          585 a          965 c          897 g          631 t
ORIGIN      
        1 atattctgcc gttcctgcag gctccagact tcctcggttt cggcgatgcg gatcaggaaa
       61 tattccatcc gctccgcgtc agacaacgtt tccaccgttt gtatttcgtg agcatcgggt
      121 gcgtaacgca ttgtggtcga ttcctgctgg ccgcattcat gcaatctgtc catccaggct
      181 gttgcaagaa ttcgtccaga gggtgatgcg cgaaaaatgg caacaactcc cgcaggatcc
      241 atcctctggt gccaaaggac ggcactgatg gattgccaat gccgtcgcca acccgacaaa
      301 acccaacacc ggctgtgtca ggttcgcgac gggagttgca atcctgtttc cagatcaatt
      361 ggtcggagcc ggccgcgaac ggccacgccg acaagaatcg aagcacttgt ccaccgcgcc
      421 ctgcatcgcc tgggcgaaga aggcaacgct gctatactgg ctttcccatc caaaccatcc
      481 ggagcgcccg atgttgcagc agttgatgct ggccacgtcc cttgccctgg gctggctgct
      541 cctcactgcc tgcaccagca atcctgccgt cgtggacgcg tccgcaacgc cgggcgcttc
      601 caatacaccc gccattccgg tgtatgtcgc cagccacggc tggcataccg gcgtgatttt
      661 gccggcagcc gggctcaatc agatatttcc acagttgcgc gcgcgctttg gggcgactcc
      721 gttttatgaa atcggctggg gcgatgcacg cttttacccg gccgacgaca tcaccagcgg
      781 gctgacgctg caggcagctt tcttttctca gggcgcagtg gtgcagatcg tggcagtacc
      841 ggccaatccg gtgcgttact ttggcaacag ccaggtgcgg gcgctgtgcc tgtcgccagc
      901 acaactggac cgcctgcagc ggtacatcat tgccagcctg cacaccgatg ccaacggcca
      961 gttgcttccg cagcagaaag gcctttacgg caacagccag ttctacgccg gcaacggccg
     1021 ctttcatctg ctgcatacct gcaacacctg gaccgccaag gcgttgcgca gcgccggatt
     1081 tgcagtctca acgctgacct tgcgcgcaga cggcgtgctg gatgcgctgg aaccggtatc
     1141 ggccgtctgt ccggccatca caacggaatg acgcctggtg ccattgtgca gggaattcag
     1201 gcttcgctag gatcgttgac tgcatggccg gacagccgca cctgctccac ctgcgggtga
     1261 aaacaacatg acggggaaaa cagcatgaaa aagcagatca tctacggcgt gctcgcgttg
     1321 ctgggcatca cgctcacctg gtaccacaat atccagtttg cgctggagca cggttccttc
     1381 gcactggacc agttcatcgc cgactgcttc gtcaatcacg ccgccagttc aattacatgg
     1441 gacgtgacaa tagcggcgat cgcgtttctc acctggtcga atatcgaatc gaagcggttg
     1501 cagatgaagg gctggattgc gatcccgttc ctgacactgg gcgtcgcgct ggcatttgcg
     1561 ctgccgctgt atctgttgtt gcgggaacgg catctggcgc gacggaaagc ggcggcgtga
     1621 ggccgccttc actgcgcaat gtgtgcgggt taacggttct tggtgggaat gaacggcttg
     1681 ccactgacgc cggccttgac ccgggtcacc tgcccgcccg ccttcagaaa agccttggtc
     1741 tgttcggcga tggtctggct ggtatccttc cggctttgat cattgctctt tgatttcgct
     1801 ggctttttgg attctggctg cataaatggg cgtcactcag ggtaatggcc gatccccatg
     1861 gtacgctctt cggcccgcaa tagctcccta aataagcacc atcaggcacg aatcgctgcc
     1921 agcgcctgac gcagatggtt cactgcggca gtcacgtcct gccacttgtc gaggccgaac
     1981 agacccaggc ggaaggttct gaaatccgct ggctcgtcac acatcagcgg tacccccgct
     2041 gcagtttgca atccctgttc agcaaacttt tttccgttat gaatcagggg atcatcggtg
     2101 tagctcacta ccacacccgg tgcttcgaag ccgctggccg ccacgctgct gaagcccttc
     2161 tctgccagca gcgcccgcac ctgcttgccc agttcccact gctgcgcccg caaggtctca
     2221 aaaccggttg cgtgggattc ggccatggtg tcgcgcagtt tgagcagcgc gtcggtgggc
     2281 agggtggcgt ggtaggcgtg gccgccgttt tcataggcgc gcatgatctg cagccatttc
     2341 agcaggtcgc aggcgaaact gctgctgcgg gtggattcga tgcgacgggc ggcgtcctcg
     2401 cccagcatca ccagcgcggc acagggcgag gcgctccagc ctttctgcgg cgcgctgatc
     2461 agcacgtcga tgccgcactg ctgcatgtcc acccagatcg cgccggacgc aacgcaatcc
     2521 agcacgaaca ggccgccgtg ttcatgcacg gcagcggaca cggactgcaa atagccatcc
     2581 ggcagaatga tgcccgatgc cgtttccacg tggggcgcga acaccagatc gggtttttca
     2641 cggcggatgg ttgccaccac ctcgtcgatg ggcgcgggtg cgaatggctg cttcgatccc
     2701 gccatcaccg gtcgcgcctt caatacggtt tcccgcgccg gaatggcacc catctcgaaa
     2761 atctggctcc agcggtaact gaaccagccg ttgcgcagta ccagacaatg cttgccgccg
     2821 gcgaactggc gcgccaccgc ttccatgccg tacgtgccgc caccgggcac cacggccacc
     2881 gcctgcgcgt gataaaccgc tttcagcgtg cccgagatat cgcgcatcgc ctgctggaat
     2941 agcgccgaca tgtggttgag ggaacggtcg gtgaagacga cggaatattc gagcaggccg
     3001 tcgggatcga ccgcggtata ttgaatggac atgaggggct ccacaaaaaa tgcagggaat
     3061 gggctagctt cccacaac
//
LOCUS       DASUEG010000563         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1419325, whole genome shotgun sequence.
ACCESSION   DASUEG010000563 DASUEG010000000
VERSION     DASUEG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419325"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFX11_10570"
     CDS             <1..1094
                     /locus_tag="VFX11_10570"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEX4939110.1"
                     /translation="HLACIEGVTFAALLRGQSSGQLQLCAQNLLEQIEMPFEFQGLSL
                     NVNAAVGIAQLPDHGENAEDLLRNAHIALEMAANAGGRVAVYSRDMDPYNSRRISLLA
                     ELRAAIERDGLQLHLQPQISLQDFSVAGAEVLVRWNHPEHGNVTPAEFIPLAERTGVI
                     HPLTYWVCRKTFELSCRLRAAGHTLGLSINISARNLPATDFTRNIARIATETGVDLHD
                     ITMELTETAVMLDPVEALRVMHELAGIGVRLSIDDFGTGYSSLSHLKQLPVDEIKIDR
                     SFVKEMQNSSDDQVIVQTTLVMGHNLGLKVVAEGIEDAATLQQLQQLGCDLAQGYHIA
                     RPMPVEDFLRWLERNGRPVSPASGSASGG"
     gene            complement(1185..2399)
                     /locus_tag="VFX11_10575"
     CDS             complement(1185..2399)
                     /locus_tag="VFX11_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX4939111.1"
                     /translation="MFRYLRFYVAPLTIMAISVGMFFGYHWLWLPAALYIATIATLDQ
                     MFPHDVEETPYDYEFLLDLALYLSTPAAIMLYFALFWVAGSGTQDALGAGALVQSLTG
                     IDVIAARNATPWYHYVFMFAAVALPATGAGGLAGHELTHRTSRPFDLWLGRITMAFNW
                     GIAFPIEHVYGHHAYVGTSKDPATAARGDSVYQHLPKAMYRTVVNAWEIESARLEKIG
                     AHFLSPKNVLLRMGAVAFATSAFAYYCAGWTGVMFHLIVCTFTKLALEVLNYVEHYGL
                     IRVPTDPVQPRHSWNCNHVVSGVFTYNLTRHSHHHADAQVPYQDLRSHSEQPEMPGGL
                     MTAYLSTLIPPLWRKKITPKLLEWDATQADKREYALIRAANSKSHWPELQQSVANTTQ
                     DARTVPQGNLGY"
     gene            complement(2479..>3226)
                     /locus_tag="VFX11_10580"
     CDS             complement(2479..>3226)
                     /locus_tag="VFX11_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4939112.1"
                     /translation="QRTQGTIVKQVALTHDITEVFIELDTTVQYRPGQYASVQAAGTD
                     IPARCYSFAHACTPGGRRDISFFIRAVPSGRMSHWLMSPAALNRKVNLHASLGDFYLR
                     DSRRDLLCVAGGSGLAPLIALLEGALENTAETGAANRNVFLLMGARSQRDLYYLDAIK
                     ALQQKWQGRFEFIPVLSEEPADSDWKGQRGWVTDAITPDIAGNAEGYLCGPPPMIDAA
                     ITCMTTLGVPRDQIYFDKFSDQTVPAQKAG"
BASE COUNT          633 a          929 c         1051 g          613 t
ORIGIN      
        1 atcatctggc ctgcatcgag ggcgtgacgt ttgccgccct gctgcggggg cagagcagtg
       61 ggcaactgca gctgtgcgcg cagaatctgc tggaacagat tgaaatgccg ttcgagtttc
      121 agggcctgag cctgaacgtg aatgcggccg tcggcattgc gcaattgccg gaccatggag
      181 aaaacgccga ggatctgctg cgcaacgccc atatcgcact ggagatggcg gccaatgccg
      241 ggggccgggt ggcggtgtat tcccgtgaca tggatcccta caattcgcgc cgtatttcct
      301 tgctggcgga actgcgcgcc gcaatcgagc gtgacggcct gcaactgcac ctgcaaccgc
      361 agatttcact gcaggatttc agtgtggcgg gggcggaggt gctggtgcgc tggaatcatc
      421 cggagcacgg caacgtgacc cctgccgaat tcattccgct ggcggagcgt accggtgtga
      481 ttcatccgct tacctactgg gtgtgccgca agacattcga actgagctgc cggttgcggg
      541 ccgccggcca caccctggga ttgtccatca atatttccgc gcgcaacctg ccggccacag
      601 atttcacgcg caacattgca cgcatagcaa cggagaccgg tgtggacctg cacgacatca
      661 ccatggagct gaccgaaacg gcggtgatgc tggacccggt ggaggcgctg cgggtgatgc
      721 acgagctggc gggaatcggc gtgcggctgt cgatcgatga tttcggtacc ggctattcct
      781 cgctgtcgca cctgaaacag ttgccggtgg atgaaatcaa gatcgatcgc agtttcgtga
      841 aggaaatgca gaacagcagc gacgatcagg tgatcgtgca gacgacgctg gtgatgggac
      901 acaacctggg cctgaaagtg gtggcggaag gcatcgagga cgcggccaca ctgcagcagc
      961 tgcagcaact ggggtgcgat ctggcccagg gttaccacat tgcccggccg atgccggtgg
     1021 aggattttct gcgctggctg gagcgcaatg gccggcctgt gtcgccagca tccggcagcg
     1081 ccagcggggg ctgagcaaca gctcgccgaa aaattctggc gcacaaaaaa agggtgaccg
     1141 ctgccaggca gggtcaccct ttttaattgc tgtaaccgat ggagtcagta acccagattg
     1201 ccctgcggca cagtgcgcgc atcctgtgtg gtattcgcca cgctctgctg cagttcgggc
     1261 cagtggctct tgctgttggc agcgcgaatc agggcatatt cacgcttgtc cgcctgggtc
     1321 gcatcccatt ccagcagttt gggcgtgatc tttttgcgcc acagcggcgg aatcagcgtg
     1381 gacagatagg ccgtcatcag accccccggc atttccggct gttccgagtg cgaacgcaga
     1441 tcctggtagg gcacctgcgc atcggcgtgg tggtgactgt gacgggtcag gttgtaggta
     1501 aacacgccgc tcaccacgtg attgcagttc caggaatgac gcggttgcac cggatcggtc
     1561 ggcacacgaa tcaggccgta atgttcaaca tagttcagca cttccagcgc cagcttggtg
     1621 aaggtgcaca cgatcaggtg gaacatcaca ccggtccagc cggcgcagta gtaggcgaat
     1681 gccgaggtgg cgaacgccac ggcgcccatg cgcagcaaca catttttcgg tgacaggaaa
     1741 tgcgcgccga ttttttccag gcgggcggat tcgatttccc acgcattcac cacggtgcgg
     1801 tacatggcct tgggcagatg ctggtaaacg ctgtcgccac gggctgcagt tgcgggatct
     1861 ttcgaggtgc ccacataggc gtggtgaccg tacacgtgct cgatcggaaa cgcgatgccc
     1921 cagttgaacg ccatggtaat gcggcccagc cacagatcaa acggacgcga ggtgcggtgc
     1981 gtcagttcgt ggcccgccag accaccggcg ccggtcgcgg gcagggcgac ggcagcgaac
     2041 atgaagacat agtgatacca gggcgtggca ttgcgcgcgg caatgacgtc gatcccggtc
     2101 aggctttgca ccagcgcgcc ggcgcccagt gcatcctgcg tgccgctgcc cgccacccag
     2161 aacagggcga agtacagcat gatggccgcc ggcgtggaca ggtacagcgc caggtcgagc
     2221 aggaattcgt agtcgtacgg cgtttcttcc acgtcatggg gaaacatctg gtccagcgtg
     2281 gcgatggtgg cgatgtagag cgctgccggc agccacaacc agtggtagcc aaaaaacatg
     2341 cccacggaaa tggccatgat ggtgagcggc gcgacataaa agcggagata acgaaacatc
     2401 gtgcagtcct ctgtcgtgat tgttgtgttg gcggtggtga taaaggcggc gtggccgcaa
     2461 aaacccgacc tgattttttt atccggcttt ttgtgccggc acggtctgat cggaaaactt
     2521 gtcaaaataa atctggtccc gtggcacacc cagcgttgtc atgcaggtga tggctgcgtc
     2581 gatcatcggc ggcgggccgc acaggtagcc ttcggcgttg ccggcaatgt ccggagtgat
     2641 cgcgtctgtc acccagccgc gctggccctt ccagtcgctg tcggccggtt cttccgacag
     2701 cacgggaatg aattcgaagc gaccctgcca tttctgctgc aaggccttga tggcgtccag
     2761 gtaatacagg tcacgctggc tgcgggcacc catcagcagg aatacgttgc ggttggccgc
     2821 gccggtttcg gccgtgtttt ccagcgcacc ttccagcaac gcaatcaatg gcgccaggcc
     2881 actgccgccg gccacgcaca gcagatcgcg ccggctgtcg cgcagataga aatcgccgag
     2941 gctggcatgc aggttcactt tccggttcag tgccgccggg ctcatcagcc agtgcgacat
     3001 gcggccgctg ggcacggcgc gaatgaaaaa ggaaatatcg cgccggccac cgggcgtgca
     3061 ggcgtgggcg aaggaatagc agcgcgccgg aatgtcggtg cccgctgcct gcacggaggc
     3121 gtactggcca ggccggtatt gcacggtggt atccagctca atgaatactt cggtgatgtc
     3181 gtgggtgagc gccacctgct tcacgatcgt gccctgggtg cgctgc
//
LOCUS       DASUEG010000564         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_510534, whole genome shotgun sequence.
ACCESSION   DASUEG010000564 DASUEG010000000
VERSION     DASUEG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510534"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1416)
                     /gene="hemN"
                     /locus_tag="VFX11_10585"
     CDS             complement(7..1416)
                     /gene="hemN"
                     /locus_tag="VFX11_10585"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX4939113.1"
                     /translation="MYLVKGGATRPSLWDSEFIRQHDVPGPRYTSYPTALQFHEEFTR
                     QDYLDAVARSNAGRKPLSIYVHLPFCQSLCYYCACNKVITQSEDKKREYLDYLIKEIK
                     QKAAEFGTDRPVFQMHWGGGTPTYYGEAELTELWYQIGRHFHLVDSDRGEYSIEIDPR
                     TISSDKLGLLKGLGFNRISLGIQDFDPRVQQAINRVQSTEQIADLMQAARSYQFRSVN
                     FDLIYGLPYQSVRSVTTTIEQVIALSPDRISLFNYAHLPERFKSQGLLPEEALPAADE
                     KLAILCAASNQLVDAGYVYIGMDHFAKPSDELAQAQMRGVLHRNFQGYTTCKEADLIG
                     LGVSAISLIDNVYCQNTKSITEYQSLLEQGQSPLLAGVALTRDDEIRRTVIMSFLCRN
                     GLEPSEVEAEYGIDFNDYFRCELGALTGFVEQGLLEQDGRDYRLTPRGRLVVRKICMA
                     FDVYLPEHMKQGRRFSRVL"
     gene            complement(1500..>2939)
                     /locus_tag="VFX11_10590"
     CDS             complement(1500..>2939)
                     /locus_tag="VFX11_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939114.1"
                     /translation="LLNAGYRVLVAESGQQALELIRNNRAVDLILMDWDLPDMNGVEA
                     SRGVREVLGAQPCPPIVLVTAYGQDDVLKEADFQPAGFLSKPINSGSLIDTVDSLLRR
                     ARGATGGLPPLVASLAVEVEARVPRILLVEDNEINRFLALENLQAVGLTVDCAENGVE
                     ALRCLDQQQYDLVLMDLQMPVMDGLECCRLIRANHTAQQLPVIAMTANAFARERQRCV
                     EVGMNGFVTKPFEPADLYQAIADNLGPAFAGWKRRLEGARPSSAGELPQGLPGIQLAS
                     ALQRIHGNLPAYARLVEEYIDQFGQAAEGLAILVSQQEWASCRNLAHRLKGVAGNLGF
                     ARVNELATRIEEACSGLESNGQGDVLRLPPLIGALGDATREVLGSAEILIQLARTAQE
                     PLPEIAPLAGEEMLEWLRSVRAKLAVSEVLDDQQIRQLRQRLAGLLAAEQLEELCHGI
                     ENFDYSAAVARLDQLLSSQNAPVVANRAG"
BASE COUNT          589 a          921 c          848 g          581 t
ORIGIN      
        1 acattattac agtacgcgtg aaaaacgtct tccctgcttc atgtgttccg gcagatacac
       61 atcaaacgcc atgcagatct tgcgtaccac caaccgcccg cgcggggtca accggtaatc
      121 acggccgtcc tgctccagca atccctgttc aacaaagccc gtcaatgcgc ccagttcgca
      181 gcggaaataa tcattgaaat caattccgta ctctgcttcc acctcggacg gctccagccc
      241 gttacgacac agaaacgaca tgatgacagt acggcggatt tcgtcgtcgc gggtcagcgc
      301 gacaccggcc agcagcggtg actgaccctg ttccagcaat gactggtatt cggttatcga
      361 cttggtgttc tggcagtaca cgttatcgat caggctgatg gcagacaccc ccaggccgat
      421 cagatctgcc tccttgcagg tggtatagcc ctggaaattg cgatgcagca cgcctcgcat
      481 ctgggcctgc gcaagctcgt cagatggttt ggcgaaatga tccataccta tatagacata
      541 gccggcatcc accagttggt tcgatgccgc acagagtatg gcgagcttct catccgccgc
      601 cggcagggct tcttccggca acagaccttg cgacttgaag cgctcgggca gatgggcgta
      661 gttgaacagc gaaatgcggt ccggcgacag cgcgatcacc tgctcgatgg tggtggtcac
      721 cgaacgcacg ctttgatagg gcaacccgta aatcagatcg aaattcaccg atcggaactg
      781 gtagctgcgc gccgcctgca tcagatctgc aatctgctcg gtggactgga cacgattgat
      841 ggcctgctgc acccgggggt caaaatcctg aatccccaga ctgatgcggt tgaaacccaa
      901 ccctttcagc aatcccagct tgtcactgct gatggtgcgc gggtcgatct cgatggaata
      961 ttcaccgcga tcggaatcca ccagatgaaa atggcgaccg atctggtacc acagctcggt
     1021 gagctccgcc tcgccgtaat aagtgggcgt tccgccaccc cagtgcatct ggaagacggg
     1081 ccggtcggtg ccaaattcag ctgccttttg tttgatttcc ttgatcaggt aatccagata
     1141 ttcgcgcttt ttgtcctcac tctgggtgat gaccttgttg caggcgcagt aatagcagag
     1201 ggactgacag aaaggcaggt gcacatagat tgacaggggt ttacggccgg cattgctgcg
     1261 ggcgaccgcg tccagataat cctgccgggt aaactcttcg tggaattgca gggcggtggg
     1321 ataagacgta tagcgcggcc ccggcacgtc gtgctgccgg ataaattcgc tgtcccacag
     1381 agaggggcgc gttgccccgc ctttcaccag atacatgccc gcttcctaat ctgaatggac
     1441 tgaaaaccac tgtatgggtg gaattatcca tgatgcggga cagggtgagt tgacgtatgt
     1501 caacccgccc ggtttgcaac gacaggcgca ttctgggacg acagtaactg atccaggcgg
     1561 gccacggcgg cgctgtaatc gaaattctcg atgccgtggc acagttcttc cagttgttcc
     1621 gcggccagaa gccccgcgag acgctggcgc agctggcgga tctgctgatc gtccagcacc
     1681 tcgctgaccg ccagtttcgc gcgcacggag cgcagccact ccagcatctc ctcaccggcc
     1741 agcggtgcaa tttccggtag cggctcctgt gcggtgcggg ccagctggat caggatttca
     1801 gcgctgccga gcacttcgcg ggtggcgtcc cccagtgcgc cgatcaacgg cggcagccgg
     1861 agcacatccc cctgcccatt ggactcgagc cctgaacaag cctcctcgat ccgggttgcc
     1921 agttcattca cacgggcgaa gcccagattg cccgccacgc ccttcaggcg gtgtgccaga
     1981 ttgcgacagg acgcccattc ctgctggcta accagaatcg ccagcccttc cgctgcctgg
     2041 ccgaactggt cgatgtactc ctccaccaga cgggcatagg ccggcagatt gccgtgaata
     2101 cgctgcaggg cagatgccag ctgaataccg ggcagaccct gtggcagctc gccagcgctg
     2161 gacggccgcg caccctccag acggcgcttc caacctgcga aggccggtcc cagattgtcg
     2221 gcaatggcct ggtagagatc ggcgggctca aaaggcttgg tgacaaagcc gttcatgccc
     2281 acttccacgc agcgctgacg ctcgcgggca aaagcgttgg ccgtcatggc gatcaccggc
     2341 aattgctggg ctgtgtggtt ggcccggatc aggcggcaac attccaaccc gtccatcacg
     2401 ggcatctgca gatccatcaa caccaggtcg tactgctgct ggtccaggca acgcagggcc
     2461 tcgacaccgt tctccgcaca gtccaccgtc agacccaccg cctggaggtt ttccagcgcc
     2521 aggaaacggt tgatctcgtt gtcttccacc agcaggatgc gtggcacccg tgcctccact
     2581 tccacggcga ggctggcaac cagcggcggc aggccacctg tggcaccgcg ggcccgtcgc
     2641 agcagactgt ccaccgtgtc gatcaggctg cccgagttga tgggtttgga caggaagccg
     2701 gccggctgaa aatccgcttc cttgagtacg tcgtcctggc cgtaggcggt gacgagcaca
     2761 atcggcgggc agggttgtgc ccccagcact tcgcgtacac cgcggctggc ttccacacca
     2821 ttcatgtccg gcagatccca gtccatcagg atcagatcga ctgcgcggtt gttccggatc
     2881 agttccagcg cctgctggcc cgattccgcc accagcaccc ggtagcctgc gttgagcag
//
LOCUS       DASUEG010000565         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_894717, whole genome shotgun sequence.
ACCESSION   DASUEG010000565 DASUEG010000000
VERSION     DASUEG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894717"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFX11_10595"
     CDS             <1..553
                     /locus_tag="VFX11_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4939115.1"
                     /translation="TALESFSNSAGQESLRQHTVATAIIASQLARFSGLDDSTCYTLG
                     LLHELGKQITMVGFPDYFAAFIDKAIRDPDGTAEEIDSMCALGETMAKCWHLPDLFLH
                     CIRHHTDPASCPGPSTAYVQLIHAAHGLANAMGYPSPGETVPEDPQGECHRYLETFPP
                     EASSSEVMTSIEQAISHNLATPA"
     gene            587..1663
                     /locus_tag="VFX11_10600"
     CDS             587..1663
                     /locus_tag="VFX11_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4939116.1"
                     /translation="MSTHTILIVDDEPANRKTIIRQFEEYDYHFMEANNGQEGLDALL
                     DCMPDLILLDIRMPVKDGFGFLEQYNDTHGRTRVPVCVMTGMGDSQTRLQSVSLGADD
                     FITKPFDPIELETRITSLLRISNFQRDMLTLNADLEKRVKLRTVKLQQALEELEESKR
                     KNFRAYREMINRISGLSSLNQPSPTRMPNRVGVCAAAIAWLMGLPAETSENIALASQL
                     HDIGMLALPERLRFSDPDSLNPLERKLYFSHALIGSQLFSDSDIPLLQLAHNICASHH
                     EHFDGTGFPNGLRSQDIPLEARIFAIADTIVAVLQSEETRAVALENLNSQLAQSAGRQ
                     FDPEIVALLLQSQETLENLIDQCV"
     gene            1668..>2692
                     /locus_tag="VFX11_10605"
     CDS             1668..>2692
                     /locus_tag="VFX11_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939117.1"
                     /translation="MRKESNDQLVLLAQMAAEKDNFSPRDLINSYLPLMLIIIVGLVL
                     TVGGIVATRNWEDQIQSTYLSGTKRIYIDTVYDRLESIRQRGRMVQALFQSSQRLDEQ
                     EFADFSRSLFDQYTNGAFVALAQLPYPADPGLRAAESAPFVAHSDDIPRGIIDSVLLS
                     PRLADLPAGSGQAERQFMLQIPADTEGRDIFLVNITLLPIASNRVQLLIISGMRKQAV
                     FDTTNIELIASEIDTIEGSARRPNSNQPGAVMPVPKQDPSDQLTIDFENMTFILSITE
                     NHNILQKSRIFKWALMAFSVLFTCLLCVQFLYAKRSVQKMATLAVQRTSDLTSINSEL
                     TDEIMNR"
BASE COUNT          626 a          832 c          701 g          533 t
ORIGIN      
        1 cacggcgctg gaatccttct ccaacagcgc cggccaggaa tcgctgcgcc agcacaccgt
       61 ggcaaccgcc atcattgcat ctcagcttgc ccgattttca ggactggatg acagcacctg
      121 ttatacgctc gggctactgc atgaactggg aaagcagatc acgatggtag gatttccgga
      181 ctacttcgcc gcttttatcg acaaggcaat tcgtgacccg gacggcaccg cggaagagat
      241 cgattccatg tgcgccctgg gcgaaaccat ggcgaagtgc tggcatttgc cggatctgtt
      301 cctgcactgc atccgccatc acacagatcc cgccagctgt ccaggaccct cgactgccta
      361 cgtgcaacta atacacgcag cgcacggact ggccaacgcg atgggttacc cctcccccgg
      421 tgaaaccgtt ccggaagatc cgcagggcga gtgccatcgc tacctcgaaa cgtttcctcc
      481 ggaagccagt tcaagcgagg tcatgaccag catcgagcag gccatcagtc acaaccttgc
      541 aacgccggcc tgacgccgga acggaacatt tgaagaggag cgaaccatgt ccacccacac
      601 catcctcatc gttgacgacg aaccggccaa ccgcaaaacc atcatccgcc agttcgagga
      661 atacgactac catttcatgg aagcaaataa cggccaggaa ggcctggatg cgttgctgga
      721 ctgcatgcct gatctgatcc tgctggacat ccggatgccg gtgaaggatg gattcggatt
      781 tctggaacag tacaacgaca cgcacggccg cacccgggtg ccggtctgcg tcatgaccgg
      841 catgggagat tcccagactc gcctgcaatc cgtcagcctt ggcgccgatg atttcattac
      901 caaaccgttt gacccgattg agctggaaac ccgcatcaca tcgctgctgc gtatcagcaa
      961 cttccagcgc gacatgctga cgttgaatgc ggatctggag aaaagagtca aactgagaac
     1021 cgtcaaactg caacaggcac tcgaggagct ggaggaatcg aaacgcaaga acttccgtgc
     1081 gtatcgggaa atgatcaacc ggatatcggg cctgtcgtcg ctgaaccagc cgtcaccgac
     1141 ccgaatgcct aaccgggtgg gcgtgtgtgc ggcggccatc gcctggctta tggggctgcc
     1201 ggctgaaacc agtgagaaca tcgccctcgc cagccagctg catgatatcg ggatgctggc
     1261 actgcccgag cgtctgcgct tttccgatcc tgacagcctc aatccgctgg aacgcaaact
     1321 ctacttcagc catgcactga tcggttcgca actgttttcc gattccgata taccgctgct
     1381 tcagctggca cacaatatat gcgccagtca ccacgagcat ttcgatggta ccggcttccc
     1441 caatggcctt cgcagccagg atataccgct ggaagcgcgg atatttgcca ttgccgacac
     1501 gatcgtggca gtgctgcaga gcgaagaaac gcgcgccgtc gctctggaaa atctgaatag
     1561 ccagctggcc cagtctgcgg gccgccagtt tgatcccgag atcgtggcgc tgttgttgca
     1621 gtcacaggaa acgctggaaa acctgatcga ccagtgcgta taagcccatg cgaaaagaat
     1681 ccaacgatca actggtttta ctggcgcaaa tggcggccga aaaagataat ttttcaccgc
     1741 gcgacctgat caacagttac cttccgctga tgctgatcat catcgtcggc ctggtattga
     1801 cagtgggtgg catcgtcgcc acccgcaact gggaagacca gattcaatcc acctatctgt
     1861 cgggcaccaa gcgcatctat atcgacaccg tatacgaccg gctggagtcc atccgtcagc
     1921 gcggccgcat ggtccaggcg ctgttccagt cctcgcaacg actggatgaa caggaattcg
     1981 cggatttctc aagatcgctg ttcgatcagt acaccaacgg cgcgtttgtg gcgctggcac
     2041 agctgccata cccggccgat cccggattgc gggcggcaga atccgcgccc ttcgttgccc
     2101 actccgacga cattccacgc ggcatcatcg acagtgtcct gctgtcaccc cggctcgccg
     2161 acctgcccgc agggtccggt caggcggaac ggcaattcat gctgcagatt ccagcggata
     2221 ccgagggccg tgatattttc ctcgtcaata tcaccctgtt gcccattgca tccaatcggg
     2281 tacagctgct gattatcagt ggcatgcgca aacaggccgt gttcgacaca accaacatcg
     2341 aactgattgc cagcgaaatc gacaccatcg aaggcagtgc acggcgcccc aacagcaatc
     2401 aacccggcgc agtcatgcca gtaccgaagc aggatccatc tgatcaactc accattgatt
     2461 tcgagaacat gacctttatc ctcagcatca cggaaaatca caacatcctg cagaaatcac
     2521 gcatcttcaa gtgggcattg atggccttca gcgtcctgtt cacctgcctg ctgtgtgttc
     2581 agttcctgta tgccaagcgg tccgtgcaga aaatggcaac cctggcggtc cagcgcacca
     2641 gtgatctcac ctccatcaac tccgagctga cggatgaaat catgaaccgg at
//
LOCUS       DASUEG010000566         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2127145, whole genome shotgun sequence.
ACCESSION   DASUEG010000566 DASUEG010000000
VERSION     DASUEG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127145"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFX11_10610"
     CDS             complement(<1..198)
                     /locus_tag="VFX11_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4939118.1"
                     /translation="MMTHERAGAIAQAESDAIQQFLTFRIGTEFFGMALTQTREILEY
                     NGVTEVPLMPKFLRGVINLRGE"
     gene            complement(195..2375)
                     /locus_tag="VFX11_10615"
     CDS             complement(195..2375)
                     /locus_tag="VFX11_10615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX4939119.1"
                     /translation="MNLDGALQTFFAEAEELLTSMEAALLRVDEGAHDAETLNEIFRA
                     AHTIKGSAGLFGLHSIVGFTHIVENLLDQVRSGTMAIDRDLLSLLLNCRDHMAGLVRD
                     VMNQEEPDVDRMSRGDQLLKQLMQRLGSGDSAEPAAASAEPVVEEVAHGVPGTWHLSL
                     RFGPDTLRNGMDPLSFIRFLHTLGEITQLVTLDDHLPEAADYDPETLYLGFEIDLLTE
                     QDRTAIDDVFEFVREGSDIRILPPDSKIEEYIQLIHSLPEAPRRLGDILVQSGALSPA
                     CLERALQRQSTQDAPQRLGEILAAQHCVPEQVIETALDQQKKVAERKAAPENRTIRVD
                     AEKLDSLINLIGELVITRQRVDLLSTQTGHVQLLESVAQLKGFTEQIRDAALALRMVQ
                     IGDTFQRFKRVVRDVAQELGKSIDLVIAGAETELDRSMVEKLTDPLTHIVRNAIDHGI
                     EPVDVRRQRGKSDTGTLRLTACHDSGNIVIEISDDGGGLNLDKIRAKAIANGLIDATT
                     NYSDHDLFQLIFHPGLSTAERVTNLSGRGVGMDVVTRNIEALQGGIDIDSVPGEGTRF
                     RIRLPLTLAIIDGFHVASRNTHFIIPQNTVLECMDLAALHQVKGRACVNLRGEQVPYI
                     RLSDLFALRDSERHGDERAKLVVVQFGQERSGIVVDKLHGEIQTVVKPLSPIFQALKG
                     IGGSSLLGTGDIAFILDIPQLIELAIARESRRVGKSAVTQERAS"
     gene            complement(2372..2686)
                     /locus_tag="VFX11_10620"
     CDS             complement(2372..2686)
                     /locus_tag="VFX11_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4939120.1"
                     /translation="MDISRKDSKHSTRISISGELTIYDVSAARARLLDDSKGWPATVN
                     LDLKGVTELDSAGVQLLLVLQKGVAAAGGQLRLPAVSEPAQQVFNTLRLGAPFTVHGE
                     SA"
     gene            complement(2699..3067)
                     /locus_tag="VFX11_10625"
     CDS             complement(2699..3067)
                     /locus_tag="VFX11_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939121.1"
                     /translation="MAKNIMIVDDSASLRQVVAIALRGAGYDVIEAQNGQDALSKLDG
                     KKIHLIISDVNMPVMDGITLVKEVKKLPAYKFTPIIMLTTEGADAKKKEGQAAGAKAW
                     IVKPFQPDQMLKAVSTLILP"
     gene            complement(3095..>3755)
                     /locus_tag="VFX11_10630"
     CDS             complement(3095..>3755)
                     /locus_tag="VFX11_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4939122.1"
                     /translation="KLSQITDELRAMGAEVAGIASQTNLLALNAAIEAARAGEQGRGF
                     AVVADEVRTLSTRSGETGSRITQRIVQVNDTLQATLERTTQFAQQEARILQQAETTIQ
                     SVLSEFRESGNQIVESAQVLEQESGQVRNNVADVLVALQFQDRVSQILGHVITDMQKL
                     ADTLGEHQQQLKDGKSVAELDVPRWLADIERTYTTLEQVAVHRGGRQQQNPADSSVTF
                     F"
BASE COUNT          619 a         1157 c         1185 g          794 t
ORIGIN      
        1 ttcgccccgc aggttgatca caccgcgcag aaatttgggc atcaggggta cttcggtcac
       61 gccgttgtat tcgagaattt cccgcgtctg cgtcagcgcc atgccaaaaa attcggtgcc
      121 gatacgaaag gtaaggaatt gctggatggc gtcggattcc gcctgcgcaa tggcgcctgc
      181 gcgttcgtga gtcatcatga ggcacgctcc tgcgtgacgg cggatttgcc gacgcgacgg
      241 gattcccgcg cgatggccag ttcgatcagt tgcggaatat cgagaatgaa ggcgatgtcg
      301 ccggtgccca gcaggctgga gccgccgatg cctttcaacg cctggaatat ggggctcagc
      361 ggtttgacta cggtctggat ctcgccgtgc aatttatcca ccacgatgcc ggaacgttcc
      421 tggccgaact gcaccaccac cagcttggcg cgttcgtcac catgccgttc gctgtcgcgc
      481 agcgcgaaca gatcgctcag gcggatgtag ggcacctgct cgccgcgcag gttgacgcag
      541 gcacgcccct tcacctgatg cagcgcagcc agatccatgc attccagcac ggtattttgc
      601 ggaatgatga aatgcgtgtt gcgtgaggcc acatgaaaac cgtcgatgat tgccagcgtc
      661 agcggcaggc ggatgcggaa acgggtacct tcgcccggca cactgtcgat gtcgatgcca
      721 ccctgcagcg cttcgatgtt gcgcgtcacc acgtccatgc cgacaccacg gcccgacagg
      781 ttggtgaccc gctcggcggt ggacagaccc gggtggaaaa tcagctggaa caggtcgtgg
      841 tcgctgtaat tggtggtagc gtcgatcagg ccgttggcga tggccttggc gcgaatcttg
      901 tcgagattca ggccgccgcc atcgtcgctg atttcgatca cgatgttgcc ggaatcgtgg
      961 caggcagtga gccgcagcgt gccggtatcg gacttgccgc gctggcgccg cacgtccacg
     1021 ggttcaatgc catgatcgat cgcgttgcgc acgatgtgag taagcggatc ggtgagtttt
     1081 tccaccatcg agcgatccag ttcggtttcc gcaccggcga tcaccagatc gatgctcttg
     1141 cccagttcct gcgcaacatc gcgcaccacg cgcttgaaac gctggaacgt gtcgccgatc
     1201 tgcaccatgc gcagggccag cgccgcatcg cggatctgtt cggtaaaacc tttcagctgc
     1261 gccaccgatt ccagcaactg cacatgaccg gtctgggtcg acaataaatc cacccgctgc
     1321 cgggtaatca ccagttcgcc gatcaggttg atcaggctgt cgagcttttc cgcatcgacg
     1381 cggatggtgc ggttttccgg cgccgccttg cgctcggcga cttttttctg ctggtccagc
     1441 gcggtttcga tcacctgctc cggcacgcag tgctgcgccg ccaggatttc gcccagccgc
     1501 tgtggcgcgt cctgcgtgct ttgccgttgc agcgcgcgct ccaggcaggc gggactgagg
     1561 gcgccgctct gcaccaggat gtcgccaagc cggcgcggtg cttccggcag cgaatgaatc
     1621 agctggatgt attcctcgat cttgctgtca ggcggcagga tgcggatgtc cgatccttcg
     1681 cgcacgaatt cgaacacatc gtcgatggcg gtacgatcct gctcggtgag cagatcgatt
     1741 tcaaaaccca gatacagcgt ttccggatcg tagtcggcgg cttccggcag atgatcgtcc
     1801 agcgtcacca gctgggtgat ctcgcccagc gtgtgcagga agcggatgaa cgacagcggg
     1861 tccatgccgt tgcgcagggt gtcgggcccg aagcgcagcg acaggtgcca ggtgccaggc
     1921 accccgtgcg ccacttcttc gaccacaggt tcagcgctgg cggcggccgg ctccgcactg
     1981 tcgccgctgc ccagccgctg catcagctgt ttcagcagct ggtcgccccg gctcatgcgg
     2041 tccacgtccg gctcttcctg gttcatcacg tcgcgcacca ggcccgccat gtgatcgcgg
     2101 cagttcagca gcaggctgag caggtcgcgg tcgatggcca tggtgccgga gcgcacctgg
     2161 tccagcaggt tttccacgat atgggtgaag ccgacgatgg agtgcaggcc gaacaggccg
     2221 gcagaaccct tgatggtgtg cgccgcgcga aaaatttcat tcagtgtttc cgcgtcgtgg
     2281 gcgccttcgt ccacccgcag cagggcggct tccatcgacg tgagtaattc ttccgcttcc
     2341 gcgaagaagg tttgcagtgc cccgtccagg ttcatgcgct ttctccgtgc accgtaaagg
     2401 gcgcgcccag gcgcagggtg ttgaacacct gctgtgccgg ttcactcacg gcgggcagac
     2461 gcaactggcc accggcggcg gccacgcctt tttgcagcac cagcagcaat tgcacgccgg
     2521 cactgtccag ttcggtgacg cccttgagat cgaggttgac ggtggcaggc cagcccttgc
     2581 tgtcgtccag cagtcgggcc ctggccgcgc tgacgtcgta gatggtgagt tcgccgctga
     2641 tgctgatgcg tgtgctgtgc ttgctgtcct tgcggctgat gtccatgggg tgcctctgct
     2701 acggcaatat cagggtggat acagccttga gcatctggtc gggttgaaac ggcttcacga
     2761 tccaggcctt ggcgccggcg gcctggcctt cctttttctt ggcgtcggcg ccttcagtgg
     2821 tgagcatgat gatgggcgtg aatttatacg ccggcagttt cttcacttcc ttcaccagcg
     2881 tgatgccgtc catcaccggc atgttcacgt cggaaatgat caggtggatt ttcttgccgt
     2941 ccagtttgct cagcgcgtcc tggccgttct gcgcctcgat cacgtcatag ccggcgccgc
     3001 gcagggcgat ggccacaacc tggcgcaggc tggcggagtc atcgacgatc atgatgttct
     3061 tggccatagc ctttccttcg tttgcaaaat gccttcagaa aaaggtgacg gaagaatccg
     3121 ccggattctg ttgctggcga ccgccgcgat gcaccgcgac ctgttccagc gtggtgtagg
     3181 tgcgttcgat gtccgccagc cagcgcggca cgtccagctc ggccacggat ttgccgtcct
     3241 tcagctgctg ctggtgttcg cccagggtgt cggcgagctt ctgcatgtcg gtgatgacgt
     3301 ggccgagaat ctggctcacc cggtcctgga actgcagcgc caccagcaca tcggccacgt
     3361 tgttgcgcac ctggccgctt tcctgttcca gcacctgggc cgattcgacg atctggttgc
     3421 cggattcgcg aaattcactc agcaccgact ggatggtggt ttccgcctgc tgcaggatgc
     3481 gcgcttcctg ctgggcgaac tgggtggtgc gttccagcgt ggcctgcagg gtgtcgttca
     3541 cctgcacgat gcgctgggtg atgcgggagc cggtttcgcc ggagcgggtt gaaagagtac
     3601 gcacttcgtc tgccaccacg gcaaaaccac ggccctgttc gccggcgcgg gcggcttcaa
     3661 tggcggcgtt cagcgccagc aggttggtct gcgaagcgat gcccgccact tcggcgccca
     3721 tggcccgcag ttcatcggtg atctgcgaca gcttg
//
LOCUS       DASUEG010000567         6844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_849733, whole genome shotgun sequence.
ACCESSION   DASUEG010000567 DASUEG010000000
VERSION     DASUEG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849733"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFX11_10635"
     CDS             complement(<1..270)
                     /locus_tag="VFX11_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017039249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4939123.1"
                     /translation="MADLFASDNQWQPLAARMRPATLDQYVGQRHLFGEGQPLRRSIE
                     AGHIHSMILWGPPGVGKTTLAMILANYVDAQFLSVSAVLSGVKEIR"
     gene            complement(294..935)
                     /gene="lolA"
                     /locus_tag="VFX11_10640"
     CDS             complement(294..935)
                     /gene="lolA"
                     /locus_tag="VFX11_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX4939124.1"
                     /translation="MVRIFLSVLLALAFAQGARAGDLSPEKASQQLNQLLTEMQSMQA
                     VFEQWVNDAKQTTLQHVTGSMWIQRPGHFRWDTDEPYPQQIVSDGKSLLIYDRDLEQA
                     TRKKLDNQIGNTPALLLSGDPQQIQQNFSVTGYVFENTGEVRFDLRPKDKEAMFSLLR
                     VHFLQGYLRDMYLEDNLGQTTRIEFTKIRKNEKLDGKVFELKLPDSVDLIEET"
     gene            complement(1037..3373)
                     /locus_tag="VFX11_10645"
     CDS             complement(1037..3373)
                     /locus_tag="VFX11_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX4939125.1"
                     /translation="MPQWSVLLVRGAREGAIIALFFLSIYLLAALATFDANDPGWSSL
                     GSARSVHNLGGPAGAWCASVLMSLFGYLAYLFPVLIAWRAWLVLRDKSAQWSWLMASL
                     RFLGFVMTMVAATGLAYVHFKVIPGTLPEIDSLGRNTAGGILGIEVGRAALSNLNALG
                     ATLFLLAMFLTGMTMFTDVSWLKVIDGTGKLTLKLIDWLKQELVRIQQNRTQPNGISA
                     TKDDEKVAKQSLKKRQESLKQDKAKQETRKPPVIELPLPDVEKSTRVEKERQKPLFEY
                     DAAAEGSLPPISLLDVADKNSKKGYSTETLESMSRLLEIKLRDFNVEAEVVNVMPGPV
                     VTRFEIQPAAGVKASRITNLAKDLARSLAVVSVRVVEVIPGKTVVGVEIPNESREIIR
                     LSEIIASQDYDKTQSPLVLALGKDISGKPVIADLQRMPHLLVAGTTGSGKSVGLNAML
                     ISMLFKATPEDLRLIMIDPKMLELSVYDNIPHLLTPVVTDMKEASNALRWCVAEMERR
                     YRLMAAMGVRNLAGYNKKVKDASKRGEPIMDPFHQPEIDSQQPRELGGLPFIVVVIDE
                     FADMMMIVGKKVEEQIARIAQKARAAGIHLILATQRPSVDVITGLIKANVPTRMAFQV
                     SSKIDSRTILDQGGAEQLLGHGDMLYLPPGTGLPVRVHGAFVDDDEVHRVCDDWRRRG
                     APDYLDEILEGNHEAGPAPGDEGGEGGGDSESDPLYDQAVEFVLESRRASISAVQRKL
                     KIGYNRAARMIEAMEAAGVVTEMQTNGQREVLAPGPHR"
     gene            complement(3573..4628)
                     /gene="ald"
                     /locus_tag="VFX11_10650"
     CDS             complement(3573..4628)
                     /gene="ald"
                     /locus_tag="VFX11_10650"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX4939126.1"
                     /translation="MKIGVPMETKPMEARVALIPYAVAELVQAGHEVLVQASAGLLSG
                     YSDAEFVAAGATVLPSAEAVFASAELIVKVKEPIAGDLALLRSQHLLFCYLHLAPNPE
                     LSRRLCDIGLTAVAFETVVEGGRLPLLAPMSEIAGRVAVQAGVHYLHRSMGGKGLLLG
                     GVPGAENGRVVIIGAGVAGQNAARMAAAMGAYVTAFDRSPDALRAIEAIAPNITGLFA
                     YQDAIATAMREADMVIGAVLIPGAKAPKLVTRDMVRAMPDGGFIADISIDQGGCIETI
                     RATDYRNPVYVEEGVMHMGVTNMPGAVPRTASQALSGAILPYVHKLAGGQWQQVPSLQ
                     QGVNVAGGRIVHPALLS"
     gene            4787..5740
                     /gene="trxB"
                     /locus_tag="VFX11_10655"
     CDS             4787..5740
                     /gene="trxB"
                     /locus_tag="VFX11_10655"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX4939127.1"
                     /translation="MSATRHTRLLILGSGPAGYTAAVYAARANLKPVMITGIQPGGQL
                     TTTTEVDNWPGDVHGLQGPALMERMKAHAERFDTEIIFDHINKADLSKRPFTLWGDSG
                     TYTCDALIIATGASARYLGLESEENFKGKGVSACATCDGFFYRKQKVAVIGGGNTAVE
                     EALYLANIASEVVVVHRREKFRAEKIMQDRLFDKAKNGNVTLELGYTLDEVLGDNTGV
                     TGMRIKHLDTGATKDIPLQGVFIAIGHTPNTGIFEGQLDMHNGYIKIRSGIEGNATAT
                     SVPGVFAAGDVTDHVYRQAITSAGFGCMAALDVERYLDEHK"
     gene            5745..6470
                     /gene="aat"
                     /locus_tag="VFX11_10660"
     CDS             5745..6470
                     /gene="aat"
                     /locus_tag="VFX11_10660"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX4939128.1"
                     /translation="MIKIPVLDHRHQDFPPIEHALAEPNGLLAAGGDLSVDRLLKAYS
                     QGIFPWFDDDQPILWWSPDPRAVLFPDQLHISRSLHKTLRSGRFTVTADTAFDAVVEA
                     CAEPRKGSSGTWITRDIRHAYGVMHQRGYAHSIECWQDGKLAGGIYGIALGNLFFGES
                     MFSRMTDASKVAVVHLVGQLQQQNCPLIDCQVMNPHLARLGATLIPRAEFKSCLQRNL
                     PSLTELRAQPARKWQLTWHYPGQ"
     gene            6576..>6844
                     /locus_tag="VFX11_10665"
     CDS             6576..>6844
                     /locus_tag="VFX11_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX4939129.1"
                     /translation="MTDLAAIKFFVTPLHQCSYLPRKDAITLFADPKANLNHNLYSEL
                     SELGFRRSGNYLYRPHCRQCCACIPVRIPADRFQMSRRQKRILQR"
BASE COUNT         1441 a         2270 c         1810 g         1323 t
ORIGIN      
        1 gcggatttcc ttcacgccgc tcaacaccgc cgacacactc aaaaactgcg catcgacata
       61 gttggccaga atcatcgcca gcgtggtctt acccaccccc ggcggccccc acaggatcat
      121 ggaatgaata tggccagcct cgatcgaccg ccgcagcggc tgaccctcgc caaacaggtg
      181 ccgctggccc acgtactggt ccagcgtcgc cggccgcatg cgggctgcaa gcggttgcca
      241 ctgattgtcg ctggcaaaca ggtcggccat aaattccttc aaatcagttg taatcaggtt
      301 tcctcgatca gatcgacgct gtccggcagt ttcagttcaa acaccttgcc gtccagcttt
      361 tcgttcttcc ggattttggt aaattcgatg cgggtggtct gacccaggtt gtcttcaaga
      421 tacatgtcgc gcagataacc ctgcaggaaa tgcacgcgca gcaggctgaa catggcttcc
      481 ttgtctttcg ggcgcaaatc gaaccgcact tcgccggtgt tctcgaacac atagccggtg
      541 acggaaaaat tctgctggat ctgctgcgga tcgccgctca gcaacagcgc gggcgtattg
      601 ccgatctggt tatcgagttt tttccgcgtg gcctgctcca ggtcgcggtc atagatcagc
      661 agggatttgc catccgacac gatctgctgc ggatagggtt catccgtgtc ccagcggaag
      721 tggcccgggc gctgaatcca catggagccg gtcacatgct gcagcgtggt ctgcttggca
      781 tcattcaccc attgctcgaa taccgcctgc atcgactgca tctcggtgag caactggttg
      841 agctgctggc tggccttttc gggactgaga tcgccagccc gcgcgccttg cgcaaacgcc
      901 agggcaagca ggacactaag gaaaatacga accatggaat taccttcaaa tactgacgcc
      961 gccggggttt cggccgcgat accaggaatg tggacagaat gaaaagatgg cggttccagg
     1021 ccggaaccgc gggtgatcag cggtgaggac cgggcgccag gacttcgcgc tggccgttgg
     1081 tctgcatttc agtcaccacg cctgccgctt ccatcgcttc gatcattctg gcggcgcgat
     1141 tatacccgat tttcagcttg cgctggaccg cggaaatgga ggcacggcgg gattccagta
     1201 cgaactcgac ggcctggtca tacaggggat cggactcgga atcacccccg ccttcgccgc
     1261 cttcatcacc cggtgcagga cccgcttcgt ggttaccttc cagaatctcg tccagatagt
     1321 caggtgcgcc acggcgacgc cagtcgtcac agacgcgatg cacttcgtca tcgtccacga
     1381 acgcaccatg cacccgcacc ggcagccctg tgcccggcgg caggtacagc atgtcaccgt
     1441 ggcccagcaa ctgttcggca ccgccctgat ccagaatggt ccgcgaatcg atcttcgacg
     1501 acacctggaa tgccatgcgg gtaggcacgt tggccttgat cagaccggtg atcacgtcca
     1561 ccgacggccg ctgggtggcc agaatcaggt gaataccggc agcacgggcc ttttgcgcaa
     1621 tccgtgcgat ctgctcttcc acctttttgc ccacaatcat catcatgtcg gcgaattcgt
     1681 cgatcaccac cacgatgaag ggcagtccgc ccagttcacg cggctgctgc gaatcgattt
     1741 ccggctggtg gaacgggtcc atgatgggct cgccgcgctt gctggcgtcc ttcaccttct
     1801 tgttgtaacc cgccaggttg cgcacgccca tggcggccat caggcgataa cgccgctcca
     1861 tctccgccac gcaccagcgc aatgcgttgg acgcttcctt catgtcggtc accaccggtg
     1921 tcagcaggtg gggaatgttg tcgtacaccg acagttccag cattttcgga tcaatcatga
     1981 tcaggcgcag atcttccggc gtggccttga acagcatgct gatcagcatc gcattcagac
     2041 caacggattt accggaacca gtggtgcccg ccaccagcag atgcggcatc cgttgcaggt
     2101 cggcgatcac cggcttgccg gaaatatcct tgcccagcgc cagcaccagc ggcgattgcg
     2161 ttttgtcgta atcctgagac gcgatgattt cactcagacg gatgatttcc cggctctcgt
     2221 tgggaatctc gacccccacc acggtcttgc cgggaatcac ttccaccacc cgcacgctca
     2281 caactgcgag cgaccgcgcc aggtccttcg ccaggttggt gatgcgactc gccttcacac
     2341 cggccgcagg ctggatttca aaccgtgtca ccaccgggcc gggcatcacg ttgaccactt
     2401 ccgcttccac gttgaaatcc cgcagcttga tttccagcag gcgggacatg ctttccagcg
     2461 tttcggtgga atagcctttc ttggaattct tgtccgccac atccagcagc gaaatcggcg
     2521 gcagactgcc ttctgcagcg gcatcgtatt caaacagcgg cttctggcgc tccttctcga
     2581 cgcgcgtact tttttccaca tccggcaacg gcaattcgat caccggtggc ttgcgggttt
     2641 cctgcttggc cttgtcctgc ttcagcgatt cctggcgttt tttcagcgac tgctttgcca
     2701 ccttctcatc gtctttcgtg gcggaaatac cattgggttg cgtccggttt tgctgaatgc
     2761 gcaccagttc ctgcttcaac caatcgatca gtttcagcgt cagcttgccg gtgccatcaa
     2821 tcaccttcag ccaggacaca tcggtgaaca tggtcatccc ggtgagaaac atcgccagca
     2881 gaaacagcgt ggcacccagc gcattgagat tggacagcgc cgcgcgtccc acttcaatcc
     2941 cgagaatgcc gccggccgtg ttgcgcccca aagaatcgat ctcaggtaac gtgccgggaa
     3001 tcaccttaaa atgcacgtac gccagaccgg tcgctgccac catcgtcatc acaaaaccga
     3061 ggaaacgcag cgacgccatc agccagctcc actgggccga cttgtcacgc agcaccagcc
     3121 atgcacgcca ggcaatcaga acgggaaaca gataagcgag ataaccaaac agagacatca
     3181 gcacactggc acaccaggca ccggcaggac cacccaggtt gtgcacgctg cgggcactgc
     3241 ccaggctgct ccagcccgga tcgttggcat cgaatgtggc cagcgccgcc agcagataaa
     3301 tggacaggaa gaacagcgcg atgatggcgc cttcgcgcgc gccccgcacc agcagaacgg
     3361 accattgggg aatgtggggt tgaacctgtt cttttttcaa aacgcgcgtc ctttaagtcg
     3421 gccggaacag acaggctccc ggccgcaatg gcttgtgatc cactgatcca ttttagcggc
     3481 aaatcatgac tttgcacaaa ctttaggcag catcgcctca ggtttgtgac ctaagcgcct
     3541 aatgttaagc acctattgta ctgattgccg ggtcaggata acaaagccgg atgcacaatt
     3601 ctgccacccg ccacattaac cccctgctgc aacgacggca cctgctgcca ttgcccgccg
     3661 gccagcttgt gcacataagg caggatggcg ccggacaacg cctgcgacgc cgtgcgcggc
     3721 acagcccccg gcatattcgt cacccccatg tgcatcacac cctcctccac ataaaccggg
     3781 ttgcggtagt ccgtggcacg aatggtttcg atgcagccac cctggtcgat ggaaatgtcg
     3841 gcgatgaatc cgccgtccgg catggcgcgc accatgtcac gggtcaccag cttgggcgcc
     3901 ttcgcaccgg gaatcagcac ggcgccgatc accatgtctg cctcgcgcat ggcggtggca
     3961 atggcgtcct ggtacgcaaa caggccggtg atattgggcg cgatggcttc aatggcgcgc
     4021 aacgcatccg gggaacggtc aaacgccgtg acataggcac ccattgcggc agccatccgc
     4081 gctgcattct gccccgccac accggcgccg ataatcacca cccggccatt ttccgcgccc
     4141 ggcacgccgc ccagcagcaa gccttttcca cccatcgacc gatgcagata gtgcaccccc
     4201 gcctgcaccg ccacccgccc ggcaatttca ctcatgggcg ccagcagcgg caagcggccg
     4261 ccctccacca ctgtctcgaa cgcgacagcg gtcagcccga tgtcacacaa gcgacggctc
     4321 aattccggat tcggcgccag atgcagatag cagaacagca ggtgttgact gcgcagcagc
     4381 gcaagatcac ccgcaatcgg ctccttcact ttcacgatca gttcggcgga agcaaacacg
     4441 gcttccgctg aaggcagaac ggtcgccccg gccgccacga attccgcatc ggaatagcca
     4501 ctcagcaaac cggccgacgc ctgcaccagc acctcatgcc cagcctgcac cagctctgcc
     4561 accgcatagg gaatcagcgc gacgcgcgcc tccatgggct tggtttccat tggtactccg
     4621 atcttcatga tcaacctccg cagatgacac aaccggtttc tgtataatca gtatagttcc
     4681 accctatatt caagattcgt actaactatt tgcgtcatat aaggcaaaga cgtataacgt
     4741 cacaccatca gaaaaccacg ttgcaacgaa ccatggaaat gtcccgatga gcgcaacccg
     4801 acacacccgc cttctgatcc tgggctctgg ccccgccggc tacaccgctg ccgtgtatgc
     4861 cgcccgcgcc aacctcaaac cggtcatgat caccggcatt cagcccggcg gccagctcac
     4921 caccaccacc gaagtcgaca actggcctgg cgacgtccac ggtctgcagg gccccgccct
     4981 gatggagcgc atgaaggccc acgccgagcg cttcgacacc gaaatcattt tcgaccacat
     5041 caacaaggcc gacctgagca aacgcccgtt cacgctgtgg ggcgattccg gcacctacac
     5101 ctgcgatgca ttgatcattg ccaccggcgc gtccgcacgt tatctgggcc tggaatccga
     5161 agaaaatttc aagggcaagg gtgtcagcgc gtgcgccacc tgcgacggat ttttctatcg
     5221 caagcagaaa gtggcggtca tcggcggcgg caacacagcg gtggaagaag cgctgtacct
     5281 cgccaatatt gcatcggaag tggtggtcgt gcatcgccgc gaaaaattcc gcgccgaaaa
     5341 aatcatgcag gaccgtctgt tcgacaaagc caaaaacggc aacgtcacgc tggaactggg
     5401 ctacacgctg gatgaagtgc tgggcgacaa caccggcgtc accggcatgc gcatcaagca
     5461 tctcgacacc ggcgccacca aggacattcc gttgcagggc gtgttcattg ccatcggtca
     5521 cacgcccaac accggcattt tcgaaggcca actcgacatg cacaacggct acatcaagat
     5581 ccgcagcggc atcgaaggca acgccaccgc caccagcgta cccggcgtgt tcgcggccgg
     5641 cgacgtaacc gaccatgtct atcgccaggc catcacgtcc gccggcttcg gctgcatggc
     5701 cgcgctggac gtggagcgct acctggacga acacaagtga tcgcatgatc aaaattcccg
     5761 tcctggatca tcgccaccag gacttccccc ccatcgagca cgccctggct gaacccaacg
     5821 gcctgctcgc tgcggggggc gacctctctg tagatcggct gctcaaagcc tattcccagg
     5881 gcatttttcc ctggttcgac gacgaccagc cgatcctctg gtggtcaccg gatccccgcg
     5941 ccgtactgtt ccccgaccag ctccacatct cccgcagcct gcataaaacc ctgcgcagcg
     6001 gacgtttcac cgtcaccgca gacaccgcct ttgacgccgt ggtggaagcc tgcgccgagc
     6061 cccgcaaagg cagcagcggc acctggatca cccgcgacat tcgccatgcc tacggcgtca
     6121 tgcaccagcg cggctacgcc cactccatcg aatgctggca agacggcaaa ctggcaggcg
     6181 gtatttacgg aattgcctta ggcaacctgt tcttcggcga atccatgttt tcccgcatga
     6241 ccgacgcttc caaggtcgcc gttgtccatc tcgtgggcca gttgcagcaa caaaactgcc
     6301 ccctgatcga ttgccaggtc atgaaccccc acctggcgcg gctcggcgcc accctgattc
     6361 cgcgcgccga gttcaaatcc tgcctgcagc gcaatttgcc gagcctgacg gaactgcgcg
     6421 cgcaacctgc ccggaagtgg cagctgacct ggcactaccc cggccaataa tcttgtgacg
     6481 ccaagactgg aaagcactgc aatttcgttt aatactggta aagacgcttc tcactgcata
     6541 accaccgggt taataaggct ttatcagaag acaccatgac ggatcttgca gcgatcaaat
     6601 tttttgtcac accgctccac caatgcagct acttgcctcg caaggacgcc atcacgctgt
     6661 tcgccgatcc aaaagccaat ctgaaccaca acctgtacag cgaattgtcc gaactgggat
     6721 ttcgccgcag cggtaattac ctctaccgcc cgcattgccg ccagtgctgc gcctgcattc
     6781 ccgtgcgtat tcctgccgac cgcttccaga tgtcgcgccg gcagaaacgc atcctgcagc
     6841 gcaa
//
LOCUS       DASUEG010000568         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_444362, whole genome shotgun sequence.
ACCESSION   DASUEG010000568 DASUEG010000000
VERSION     DASUEG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444362"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /gene="gcvT"
                     /locus_tag="VFX11_10670"
     CDS             complement(<1..302)
                     /gene="gcvT"
                     /locus_tag="VFX11_10670"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015067781.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX4939130.1"
                     /translation="MGHRTALYQTHLNAGAKMVDFGGWDMPIHYGSQIEEHNAVRGDA
                     GMFDVSHMTVVDVTGSGCKAYLQKLLANDVAKLTSPGKALYSGMLNEKGGVIDDLIV"
     gene            complement(461..2080)
                     /locus_tag="VFX11_10675"
     CDS             complement(461..2080)
                     /locus_tag="VFX11_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4939131.1"
                     /translation="MTQPGLLKRIGLLRLCAVLLALMVMAPLLVIALSWGTVQTDIWA
                     HLAQTQLTRLLGNTLVLVLGVGSSVLVLGVALAWLTATCQFPGRKLFDVALMLPLAVP
                     AYVMAFVMVGLLDWAGPVQSQLREWFGRGVSFSFRGTGAVIMVLSLVLYPYVYMLARN
                     AFLAQGREPLEAARVLGHSPLRGFFLVALPMARPAIAAGVALALMETLADFGAVSVFN
                     YDTFTTAIYKAWYGFFNVQAAAQLASLLLVIVLAGLFLERKARGDARYYQSARRKALL
                     HRTRLTGARGWLATAFCGLIFLLAFGIPIIQLLVWVGQGAWQDLDERYAALVWHTLGL
                     GGGAALLTVAAAFLLAYFKRRYADAFAATLVRLASLGYALPGSVLAVGIMISFNHLDD
                     WAAQAQTALGIPVQPVFVGGVLALLLAYVTRFLAAAFGPVETSLERIKPSIPEAAAIL
                     GARQWEIVRRIYLPMLAPGLLTAGLLVFVDVMKEMPATLLMRPFGWDTLAVRIHEMTS
                     EGLWQRAALPGLTLVLVGLLPVAILVRRSGR"
     gene            complement(2126..>2604)
                     /locus_tag="VFX11_10680"
     CDS             complement(2126..>2604)
                     /locus_tag="VFX11_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4939132.1"
                     /translation="HGEAKTEQLVKGWVDNLATDVFANDTALIEAIVAGQCDVGIVNT
                     YYFGRLEQEKGKLPAKLFWPNQKTTGVHVNVSGAGVTTHAKHPEEAQKLLEWLAKGQA
                     QRIFAAINMEYPVNENTKWDPIVEAWGEFKADELNVEVAGKRQVEAVKLMDRAGYL"
BASE COUNT          533 a          874 c          774 g          423 t
ORIGIN      
        1 acgatcaggt cgtcgatcac gccgcccttt tcgttcagca tgccgctgta gagcgccttg
       61 ccggggctgg tgagtttggc cacgtcgttg gccagcagtt tttgcaggta ggctttgcag
      121 ccgctgccgg tgacgtccac cacggtcatg tgggacacgt cgaacatgcc ggcatcaccg
      181 cgcacggcgt tgtgttcttc aatctgcgag ccgtaatgga tgggcatgtc ccagccgccg
      241 aaatccacca tcttggcgcc ggcgttcagg tgagtctgat aaagggcggt gcggtgtccc
      301 atgaatggct cctgatgccg ggatcgaaag tggcacgatt gtaccaactt aaaatctcag
      361 ggtaaatgaa aggacgcaag ggtgtttttg ggtgtggaat gggtgcgcat tgtagcggcg
      421 cgaaggccgg ggcgaaagcc ggaccggtga atggtggtat ttaacgcccc gaccgccgta
      481 ccagaatggc caccggcagc agccccacca gcaccagggt cagacccggc agggcggccc
      541 gctgccacaa tccttcggac gtcatttcat gaatccgcac cgccagggtg tcccagccga
      601 acggacgcat cagcagggtg gcgggcattt ccttcatcac gtcgacgaat accagcaggc
      661 cggcggtgag caggcccggg gccagcatcg gcagatagat acgacgcacg atttcccact
      721 ggcgcgcgcc cagaatggca gcggcttcgg gaatggaggg cttgatccgt tccagactgg
      781 tttccaccgg cccgaacgcg gccgccagaa aacgggtgac gtaggccaac agcaacgcca
      841 gcacaccgcc gacaaacact ggctgcaccg gaatacccag cgcggtttgc gcctgcgcgg
      901 cccagtcgtc cagatggttg aagctgatca tgatgcccac cgccagcacc gatcccggca
      961 gggcataacc gagactggcc agccgcacca gcgtggcggc aaacgcgtcg gcatagcggc
     1021 gcttgaaata ggccagcagg aacgcagcag ccacggtgag cagtgcggcg ccacccccca
     1081 atcccagcgt gtgccacacc agcgcggcat agcgctcatc cagatcctgc cacgcgccct
     1141 gccctaccca caccagcaac tggatgatgg gaatgccgaa cgccagcagg aaaatcaatc
     1201 cgcaaaaagc ggtggccagc cagccgcgcg caccggtcag ccgggtgcga tgcagcagcg
     1261 ccttgcgccg ggccgactgg taatagcggg catcgccgcg cgccttgcgc tccagaaaca
     1321 gccccgccag tacaatcacc agcagcagcg acgccagctg cgccgctgcc tgcacattga
     1381 aaaatccgta ccacgcctta tagatcgccg tggtgaacgt gtcgtaattg aagacggaca
     1441 cggcgccaaa atccgccagg gtttccatca gcgccagcgc caccccggcc gcaatcgcag
     1501 gacgcgccat cggcagcgcc accagaaaaa atccgcgcag cggactgtgg ccgagcaccc
     1561 gcgctgcttc cagcggctcg cgcccctgcg ccagaaaggc attgcgggcc agcatataaa
     1621 cgtaggggta cagcaccagc gacagcacca tgatcaccgc gccggtgccg cgaaaactga
     1681 acgacacgcc gcggccaaac cattcccgca attgcgactg caccggcccg gcccagtcca
     1741 gcagccccac catcacgaac gccatgacat aggccggcac tgccagcggc agcatcagcg
     1801 ccacgtcgaa cagcttgcgg ccggggaact ggcaggtggc ggtgagccag gccagcgcca
     1861 cgcccagcac cagcacgctg ctgccgacac ccagcaccag caccagcgta ttgcccagca
     1921 gacgcgtcaa ctgtgtttgc gccagatggg cccagatgtc ggtctgcacc gtgccccagc
     1981 tgagcgcgat caccagcagc ggcgccatca ccatcagcgc cagcaacacc gcgcacaaac
     2041 gcaacagccc gatccgcttc agcagaccgg gctgtgtcat cgaatgcgcg tggtgcagcg
     2101 tggcagcggc gctcatgggc ggcgcttaca ggtaaccggc gcggtccatc agtttgaccg
     2161 cctccacctg gcgcttgccc gccacttcca cattcagttc atccgccttg aactcacccc
     2221 aggcttccac gatcggatcc cacttggtgt tttcgttcac cggatattcc atgttgatgg
     2281 cggcgaaaat gcgctgggcc tgacccttcg ccagccattc cagcagtttc tgcgcttctt
     2341 ccggatgctt ggcgtgggtg gtgacaccgg cgcccgacac gttcacgtgc acaccggttg
     2401 ttttctggtt gggccagaac agcttggcgg gcagtttgcc tttttcctgc tccagacggc
     2461 cgaaatagta agtattgacg atgcccacat cgcactggcc ggcgacaatg gcctcgatca
     2521 gcgcggtatc gttggcgaac acgtcggtgg ccagattgtc gacccagcct ttcacgagct
     2581 gctcggtttt cgcttcacca tgac
//
LOCUS       DASUEG010000569         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2082605, whole genome shotgun sequence.
ACCESSION   DASUEG010000569 DASUEG010000000
VERSION     DASUEG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082605"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFX11_10685"
     CDS             <1..552
                     /locus_tag="VFX11_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939133.1"
                     /translation="IGTGFFWLCQNWLQFPLAPVKLGSFTWRSPCSALREQQDRELAT
                     LLAETVLSPRNIARTLILGSKSRHAQTIIKKRIAPLMDDLNVRTVAQLTVGPMGYVNL
                     KQSLSDKLTESFMEPFEDEDFNQARGKVLTEFLLTRIEKQPDQLFYQQMKWVLDPLAI
                     AGTCGGFILGGLLGSLQWLLFSL"
     gene            572..1012
                     /locus_tag="VFX11_10690"
     CDS             572..1012
                     /locus_tag="VFX11_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4939134.1"
                     /translation="MAKYIFKIRWEKAPDGGETYSWHFDNGVILKADRGAAMIPGIVE
                     PEAAFVASLASCHMLSFMAQAAKRGLAVIRYHDTATGVLEKNRAGRICVTRVLLQPQV
                     EFSVPVDTTTLQELQDAAHAKCFIANSSTAQVMIDSTLLPASVI"
     gene            1014..1580
                     /gene="yeiP"
                     /locus_tag="VFX11_10695"
     CDS             1014..1580
                     /gene="yeiP"
                     /locus_tag="VFX11_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HEX4939135.1"
                     /translation="MPKACDVKKGHVIAINDQPYIVKNIEVRSPSARGAATLYKMRLN
                     HAQTGQKLDETFTGDDMLKSVDFSRREVQYLYIDDNSYVFMDKEDYSQHGLDADTLED
                     QIPYLTDNLEGIQALFVEDRCVGIELPQSVNLEIVETAPNMKGASATGRTKPAKLTTG
                     LEIQVPEYMEAGEVVRVNTQTGKFMSRA"
     gene            1639..1842
                     /locus_tag="VFX11_10700"
     CDS             1639..1842
                     /locus_tag="VFX11_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX4939136.1"
                     /translation="MLKGLTYFWCSCGLSQRQPFCDNRHEGTAFQPVPFTAPKDEILF
                     FCACKHSTTGVECDGKSHQRFLR"
BASE COUNT          458 a          636 c          556 g          380 t
ORIGIN      
        1 atcggcaccg gttttttctg gctgtgccag aactggctcc agtttccgct ggcgccggtg
       61 aaactgggtt cctttacctg gcgcagcccc tgctccgccc tgcgcgaaca acaggatcgc
      121 gaactggcca ccctgttggc agagaccgtt ctgtcaccgc gcaacattgc ccgcacgctg
      181 atactcggca gcaaatcccg ccacgcacag accatcatca aaaaacgcat tgcaccgctg
      241 atggatgatc tgaacgtgcg cacggttgcc cagctgaccg tggggccgat gggctatgtc
      301 aacctgaagc agtcgctgtc ggacaagctc acggaatcct tcatggaacc gttcgaggac
      361 gaagatttca atcaggcccg cggcaaggta ttgacggaat ttctgctgac ccgcatcgaa
      421 aaacagccgg atcagctatt ctatcagcag atgaaatggg ttcttgatcc attggcgatc
      481 gccggcacct gtggcggctt catccttggt ggattgttgg gcagcctgca atggctgctg
      541 ttttcgctct gatccctgct gcaggcacga catggccaaa tacattttca agattcgctg
      601 ggaaaaagca ccggacggtg gcgagactta cagctggcat ttcgacaatg gtgtcattct
      661 caaggccgat cgcggcgccg cgatgattcc cggcattgta gaaccggaag cggccttcgt
      721 cgcctcgctg gccagctgtc acatgctgtc attcatggcg caagccgcca agcgcgggct
      781 ggccgtcatc cgctatcacg acacagccac cggcgttctg gaaaagaacc gcgccggccg
      841 catctgcgtc acccgcgtac tgctgcaacc acaagtagag ttttccgtgc cggtggacac
      901 gaccaccctg caggaattgc aggacgccgc ccacgccaaa tgcttcattg ccaactcatc
      961 caccgcccag gtgatgatcg attccaccct tctgcccgcc agtgtgatct gacatgccca
     1021 aagcctgcga cgtcaaaaaa ggccacgtca ttgccatcaa cgaccagccc tatatcgtga
     1081 agaacattga agtgcgcagc ccgtcggcac ggggcgcggc cacgctgtac aagatgcgcc
     1141 tgaatcacgc ccagaccggg cagaaactgg acgagacgtt caccggcgac gacatgctca
     1201 agtcggtgga tttttcccgc cgcgaagtgc agtacctgta catcgacgac aacagctatg
     1261 tcttcatgga caaggaggat tacagccagc acggcctgga tgccgatacg ctggaagacc
     1321 agattcccta tctgaccgac aacctggaag gcattcaggc actgttcgtg gaagaccgct
     1381 gtgtgggtat cgaactgccg cagtcggtga atctggaaat cgtggagacc gcgccaaaca
     1441 tgaagggcgc atccgcgacc ggccgcacca aacccgcgaa gctgaccacc gggctggaaa
     1501 tccaggtgcc ggaatacatg gaagcgggtg aagtggtgcg ggtgaatacc cagactggca
     1561 aattcatgtc ccgtgcctga gcaagcctct ggcccgacat tacccagggt tgccaacacc
     1621 cggccatgcc cggtgcgggt gctgaagggc ctcacctatt tctggtgcag ctgcgggctg
     1681 agccagcgcc agccattctg cgataaccgt catgaaggaa ccgccttcca gccggttccc
     1741 tttacggccc ccaaagatga gatcctgttt ttctgcgcct gcaaacacag cacaaccggc
     1801 gtggagtgcg atgggaagag ccatcagcgg tttctgagat agccatcagc ggtttttgag
     1861 atagccatct gcagttctga aaatagccat cagcggctta acgaacagcc ttcctgcgcg
     1921 accacaccag cgccagcacc gccaccagcg caacggccag gaccggcacc gcgacccaat
     1981 tcaccatctg ccagccaaac cgctccagca aactgcctgc ggcaaacgac
//
LOCUS       DASUEG010000570         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1863936, whole genome shotgun sequence.
ACCESSION   DASUEG010000570 DASUEG010000000
VERSION     DASUEG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863936"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1558
                     /gene="murJ"
                     /locus_tag="VFX11_10705"
     CDS             2..1558
                     /gene="murJ"
                     /locus_tag="VFX11_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4939137.1"
                     /translation="MLRSTGIVSVFTLLSRVLGLVRDVVVAHYFGAGGGADAFFVAFK
                     IPNFFRRLFAEGAFSQAFVPVLSEYRTKRELLEVRLLVSHTAGSLGGVLMLVTLVCVI
                     FAPQVTFLFAPGFREAAEKFDLASDMLRLTFPYLLLISLTGFVGSILNSYGRFGVPAF
                     TPVLLNVVLILSAIFLSPFIDPPVVALAWGVLMAGVVQLVFQLPYLRPIGLLVRPRWG
                     WQDEGVKRILKLMVPALFGVSVGQINLLLGSVLASFLADGSVSWLYYADRLMEMPLGV
                     FGIAIATVILPSLSRKHASQSTEAFSATLDWAVRMIMLIGVPAGLALVILAVPLLVTL
                     FQYGEFSSNDVEQSARALQAYSLGLLGFMLIKVLAPGYFARQDTSTPVKIGVWSMVAN
                     MLLSLALIVPLTHTGLALATSAAAFLNAGMLYSGLRKAGVYVPGKGWLMFFTRLCLAS
                     LAMSVMLVWLDRSLTDWLAWGWRDRVLWLTLQVVAGIATYFVVLFACGVRVRHLRSRA
                     APDAHEDGSG"
     gene            1642..2565
                     /gene="ribF"
                     /locus_tag="VFX11_10710"
     CDS             1642..2565
                     /gene="ribF"
                     /locus_tag="VFX11_10710"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4939138.1"
                     /translation="MELIRGVHNLRPRHRGCVATIGNFDGVHCGHRAIVAQLAQKAQA
                     LGLPSVVMVFEPQPREFFAPDLAPARLMTFREKCEVLSALGVDGVLCVRFNSRFSAQS
                     AREFCDEILLNGLGIRHLVVGDDFRFGHDRSGDFAFLQAVGKEAGFSVEHTCTYELAG
                     ERVSSTRVRECLERSDFDMAATLLGQPFFMSGRVMHGQKLGRQLGVPTANLLPKRIRT
                     PIRGVFAVEVAGLGGIRRGVANLGTRPTVSGERVRLEVHLLDFDQDIYGRHVQVIFRH
                     KIRDEKKFDGLDALKAGIQQDIQTARTFFRI"
     gene            2621..>2815
                     /locus_tag="VFX11_10715"
     CDS             2621..>2815
                     /locus_tag="VFX11_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4939139.1"
                     /translation="MADYKATLNLPNTAFAMKANLAQREPELLKHWQQIDLYGQIQAI
                     GKGRPQFILHDGPPYANGEIH"
BASE COUNT          433 a          742 c          959 g          681 t
ORIGIN      
        1 attgttgcgg tccaccggca tagtcagtgt ttttaccctg ttgtcgcggg tgttggggct
       61 ggtgcgggat gtggttgtgg cccactactt tggcgcgggc ggtggcgcgg atgcgttctt
      121 tgtagcgttc aaaattccca attttttcag gcgtctgttt gctgaagggg ccttttctca
      181 ggccttcgtt ccggtgttgt cggagtaccg tacgaaacgc gagttgctgg aagtccgcct
      241 gctggtgagt catacggcgg ggtcgctcgg cggcgtcctg atgctggtca cgctggtctg
      301 tgtgatcttt gcgcctcagg tcacctttct gtttgcgccc ggttttcgcg aggcggcgga
      361 gaagtttgat ctggcgtcgg acatgctgcg gctgactttt ccttatctgc ttctgatttc
      421 cctgactggc tttgtcggtt ccatcctcaa cagttacggc cgtttcgggg tgccggcctt
      481 tacgccggtg ctgctgaacg tggtgctgat actgagcgcg atttttcttt ccccttttat
      541 cgatcctccg gtggtggcgc tggcctgggg ggtgttgatg gcaggtgtgg tgcagctggt
      601 gttccagttg ccgtatctgc ggccgatcgg attgctggtg cgcccgcgct ggggttggca
      661 ggatgagggg gtgaagcgca tcctgaaact gatggtgccg gccctgttcg gggtgtcggt
      721 ggggcagatc aatctgctgc tgggctcggt gctggcatcc tttctggcgg atggtagcgt
      781 gtcctggctg tattacgccg accggctgat ggagatgccg ctgggggtgt tcggaattgc
      841 gattgccacc gttatcctgc ccagcctgtc gcgcaagcat gccagccaga gcacggaggc
      901 gttttctgcg acgctggact gggctgtgcg gatgatcatg ctgatcgggg tacccgccgg
      961 gttggcgctg gttatcctgg cggtacccct gttggtgacc ctgttccagt acggggaatt
     1021 ttcgtccaat gacgtggagc agtcggccag ggcgttgcag gcctacagtc tggggctgct
     1081 ggggttcatg ctcatcaaag tgttggcgcc gggctatttt gcccgtcagg acacgtcgac
     1141 accggtgaag attggagtgt ggtcgatggt ggccaacatg ctgttgagcc tggcgctgat
     1201 cgtccctctg acccatacag gcctggcgct ggcgacctcg gcggcggcgt tcctgaatgc
     1261 cggcatgctg tacagcggac tgcgcaaggc gggggtgtat gtgccgggca agggatggct
     1321 gatgtttttt acccggttgt gtctggcgtc cctggcgatg tcggtgatgc tggtgtggct
     1381 ggaccggagt ctgacggact ggctggcctg gggctggcgc gatcgggtgt tgtggctgac
     1441 gttgcaggtg gtggctggaa tcgccacgta ttttgtggtg ttgttcgcgt gcggcgtgcg
     1501 ggtgcggcat ttgcggtccc gtgccgcgcc ggatgctcat gaggatggat cgggctaaat
     1561 caccccagtt gcgtcgactg ccggttaaat cagctggcca actccgctat aatgcgggac
     1621 ttttgtcagc acggcatggt tatggagttg attcgcggcg ttcacaatct gagaccccgg
     1681 caccggggct gcgttgccac catcggcaat ttcgatggcg ttcattgtgg gcatcgcgcc
     1741 atcgtcgcgc agctggcgca gaaggcccag gcgctgggcc tgccgtcggt ggtgatggtg
     1801 ttcgaacccc agccgcgcga gttcttcgcg ccggacctgg cacctgcccg cctgatgact
     1861 ttccgggaaa aatgcgaagt gctgtctgcg ctgggcgtgg acggggttct ctgcgtgcga
     1921 ttcaacagcc gcttcagtgc ccagtctgcg cgcgaattct gtgatgagat tctgctgaat
     1981 ggcctgggta tccgccatct ggtggtgggc gacgatttcc ggtttggcca tgatcgcagt
     2041 ggcgattttg cctttctgca ggccgttggc aaggaagcgg gattcagcgt cgagcacacc
     2101 tgcacctacg agctcgcggg tgagcgggtc agcagcacgc gggtgcggga gtgcctggag
     2161 cgttctgact ttgacatggc agcaacactg ctgggccagc cgtttttcat gtccggccgt
     2221 gtcatgcacg ggcagaagct gggacgccag ctgggggtgc ccacggcgaa tctgctgccc
     2281 aaacggattc gtacgccgat tcgcggcgta tttgccgttg aagtggcggg gctgggaggc
     2341 attcgccgcg gagtcgccaa tctgggtacc cgtcccacgg tgagcggcga gcgggtgcgg
     2401 ctggaagtgc acctgctgga tttcgatcag gatatttatg gccgtcatgt gcaggtcatt
     2461 ttccgtcaca agatccgtga tgagaagaaa ttcgatggac tggatgcgct gaaagccggc
     2521 atccagcagg acattcagac cgcgcggacg tttttccgga tctgacggtt ttcaacaatc
     2581 ccacgcaacg acattttctg acacaggcaa acaaaccgaa atggccgact acaaagcaac
     2641 gctgaatctg cccaacaccg ctttcgcgat gaaagccaat ctggcgcaac gggaaccgga
     2701 gctgctcaag cactggcaac agattgactt gtacggccag attcaggcga tcggcaaggg
     2761 ccgtccgcaa ttcattctgc acgacggtcc tccgtatgcg aacggcgaga ttcac
//
LOCUS       DASUEG010000571         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1659904, whole genome shotgun sequence.
ACCESSION   DASUEG010000571 DASUEG010000000
VERSION     DASUEG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659904"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFX11_10720"
     CDS             complement(<1..652)
                     /locus_tag="VFX11_10720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939140.1"
                     /translation="MSNATVLVVPGFHGSGPGHWQSWLETQLPEARRVGGIDWEQPVL
                     ADWALAIRDEIDAATGAVWIVAHSFGCLASIVAMADRTEKVAGAILVAPADPNRFSPV
                     GLRDQNRFERRSSAVNSVKSLLPDWPLPVMGLVVASSTDPWMAQSDAREWARRWGFVF
                     HDAGDAGHINVESGHGPWPLVRNLLQSLRLAAHAFPVLGSVDCAARRRGRGSVLANV"
     gene            complement(787..2544)
                     /locus_tag="VFX11_10725"
     CDS             complement(787..2544)
                     /locus_tag="VFX11_10725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4939141.1"
                     /translation="MDVQIPGYTVIRPIGQGGMAIVYLAYQHNLERQVALKVLTPALA
                     ANPDFCGRFMREAKIGASLCHRNIVPVFDVGHYQLHQYLAMEYLPGGDLKRHVAAGCD
                     TGAVLRIVYDIACALQYAHSKSYIHRDIKPENILFREDRTAVLTDFGIAVDAKSAADV
                     SSGDRVIAGSPNYMSPEQARADGVDARSDVYSLGAVLYEALSGRPLLHAGATVADVLR
                     HSSDPVPRLPSEVSWLQPVLERMLARNRDERFADMDEVMRALEPCLKVPVSLLGMPRQ
                     RVAEVAQSVQPVPMRRPALAAASSPEKPPRSPSAPTLIEDLSEDLELELALATCPPVM
                     SPALARIPPQPRRSRAGILLFLLVLSSLPLAWRHGPALLENARQLANQSAVAALPPDS
                     QAPDVRMAPPAPAPAPEIVAAPAGPVDEPPATLAAAAAPESASEATTVAPETVATESP
                     QPATPQVAEAEKKEPAPDPVEQLLQQADDALQNAQPPLLAVTSAHEKYRAVLSLDPRN
                     RPARQGLAVVASRYLELASKALNDDELESAQQYVDRASSIATRHRLGDQITQRIGDLQ
                     YVLNRIRNLDGQRRPESWS"
     gene            complement(2771..>4093)
                     /gene="glpK"
                     /locus_tag="VFX11_10730"
     CDS             complement(2771..>4093)
                     /gene="glpK"
                     /locus_tag="VFX11_10730"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX4939142.1"
                     /translation="NAQLSAADIAGIGITNQRETTVVWDRASGKPVHRAIVWQDRRTA
                     ETCVSLRSGGKGAGGKGSNGHEALVQEKTGLLIDPYFSATKIAWILDHVDGARARAEQ
                     GELAFGTIDTFLLWRLTGGKSHKTDATNASRTLLFNIHTQQWDDELLSLFNVPKALLP
                     DVEDCAADFGSTDPSLFGGAIPVAGMAGDQQAALIGQACFKPGMIKSTYGTGCFMILN
                     TGSEALQSRNRLLTTVGYRLKGQVTYGLEGSIFIAGAAIQWLRDGIALISHARETEAM
                     AQEVGVEHGVYMVPAFTGLGAPYWDPEARGALFGLTRDTGIKDIVTAGLQSVAYQTKD
                     LIHAMEGDGAGKPTTLRVDGGMVANNWVVQFLCDVLNVNVDRPAIIETTALGAAYLAG
                     LQVGVYKSLEEIAELWHCQRHFTPQMDDVLRNRLYSGWLDAVKRVRRS"
BASE COUNT          776 a         1357 c         1222 g          738 t
ORIGIN      
        1 gcacattcgc cagcacgctg cctcttccgc gccggcgcgc cgcacagtcc accgacccca
       61 gcaccggaaa cgcgtgcgcc gccagccgca ggctctgcaa caggttgcgc accagcggcc
      121 agggcccgtg cccggattcc acattgatat ggccggcatc accggcgtca tgaaacacaa
      181 aaccccagcg ccgcgcccac tcgcgggcgt cgctctgcgc catccaggga tcggtgctgc
      241 tcgccaccac cagccccatc accggcaatg gccagtccgg caacagggat ttcacactgt
      301 tgacggcgga agagcggcgt tcaaaccggt tctggtcgcg caggccaacg ggggaaaaac
      361 ggttgggatc tgccggtgcc accaggatcg cacccgccac tttctcggtg cggtcggcca
      421 tggcgacgat gctggccagg caaccgaaac tgtgggccac aatccagacc gcgccagtag
      481 cggcatcgat ctcgtcgcgg atcgccagcg cccagtccgc cagcaccggc tgttcccagt
      541 cgatgccacc cacccgccgc gcttccggca actgggtttc cagccagctc tgccagtggc
      601 ccgggccact gccgtgaaat cctggcacca ccaacaccgt tgcattgctc atctgccacc
      661 tcccggctcc aataaccgat cactctaagg ggcggcagat ggcaaacaaa ccaagaaaaa
      721 tggcaatgct tattcaggat tggaaaaaca accgcgggaa cagcccgcga ctcagtaaca
      781 cagggttcag gaccaggatt ccggccttct ttgtccgtca agattgcgaa tgcggttgag
      841 cacatactgc agatcaccga tgcgctgcgt tatctgatca cccaggcggt gccgggtggc
      901 gatactgctg gcgcgatcca catactgctg cgcgctttcc agctcatcgt cgttcaatgc
      961 cttgctcgcc agttccagat agcggctggc aacaaccgcc agcccctgcc gcgccggcct
     1021 gttacgggga tccagcgaca gcacggcgcg gtatttctca tgcgcgctgg tcactgccag
     1081 cagcggaggc tgggcgttct gcagggcgtc atcagcctgc tgcagtaact gctccacagg
     1141 atcgggtgcc ggctcctttt tttctgcctc ggcgacctgt ggcgtagcgg gctggggcga
     1201 ctcagtggcg accgtttccg gcgcgacggt tgtggcctca ctggcggatt ccggcgcagc
     1261 tgcagcggcc agggtcgcgg gtggttcatc cacagggcca gccggagcag cgacgatttc
     1321 cggcgccggt gctggtgccg gaggcgccat tcgaacgtcc ggcgcctggg aatcaggggg
     1381 aagcgctgcc acggcagact gattcgccag ctgccgcgcg ttttccagca aggccggacc
     1441 atgacgccag gccagcggca atgacgacaa caccagcagg aacagcagaa ttcccgccct
     1501 ggagcggcgc ggttggggag gtatccgcgc cagtgcggga ctcatcaccg gcggacaggt
     1561 cgccaacgcc aattccagct ccagatcttc gctgagatct tcgatcaggg tgggggcact
     1621 gggtgaccgc ggcggctttt cggggctgct ggcagccgcc agggcaggcc gccgcatcgg
     1681 cacgggctga acactctgcg caacctctgc cacccgctga cgcggcatgc ccaacaggga
     1741 caccggcact ttcaggcagg gctccagggc ccgcatcact tcatccatgt cggcaaaacg
     1801 ctcgtcacgg tttctggcca gcatgcgctc cagcaccggc tgcagccagg agacctcgga
     1861 cggcagtcgt ggaaccgggt ctgagctgtg gcgcaggaca tcggccaccg ttgcgccggc
     1921 atgcaacagc ggacgcccgg acagcgcctc gtacaacacg gcacccaggc tatagacatc
     1981 gctgcgggca tccacgccat ctgcccgggc ctgctccggg ctcatgtaat tgggcgagcc
     2041 ggcaatcacg cggtccccgc ttgacacgtc ggcagcggat ttcgcatcca cggcgatgcc
     2101 gaaatccgtc agaaccgccg tccggtcttc ccggaacaga atgttttcag gcttgatatc
     2161 gcgatggata taggattttg aatgggcgta ctgcagtgcg caggcaatgt catagactat
     2221 ccgcagcacc gcacccgtat cacagccggc cgccacatga cgcttgagat ccccgcccgg
     2281 caggtattcc atggcgaggt attgatgtaa ctggtaatgg cccacatcga acacgggcac
     2341 aatattgcga tgacagagac tggcaccgat cttggcttcc cgcatgaagc gcccgcagaa
     2401 atccggattc gccgccaacg ccggcgtcag gactttaagc gctacctgcc gttcaagatt
     2461 gtgctggtag gccagataaa ctatggccat cccaccctgg ccaatcggtc tgataacggt
     2521 gtagccggga atttgcacgt ccatataatc accatccgtt cgtaagtagc acagaaccgg
     2581 atgagtattg ttggcctata agtcttgcga tgtccatgcg cggttaacac aattgcaaca
     2641 tcccggcgcg aattgcgaaa acggtggatg ctgctgctcg cgaatcgtgc agaaaatgcg
     2701 atttttggcc ggaatttgtg cgtttctcac cgatgacaga cgcacgtcga ctgtcatcgg
     2761 tggtaattca tcaactgcgc cgcacccgtt tcacggcatc cagccagcct gaatacaacc
     2821 ggttgcgcaa tacatcgtcc atctgtggcg tgaaatgacg ctggcagtgc cacaattcgg
     2881 caatctcctc cagcgacttg taaaccccca cctgcagtcc tgccagatag gccgccccca
     2941 gcgccgtggt ctcgatgatc gccgggcgat ccacattcac gttcaacacg tcacacagga
     3001 actgcaccac ccagttgttc gccaccatgc cgccgtccac gcgcaacgtg gtgggcttgc
     3061 cggcgccgtc cccttccatg gcgtgaatca ggtccttggt ctggtacgcc accgactgca
     3121 gtccggcagt gacaatatcc ttgatgccgg tgtcgcgggt caggccgaac agggcgccgc
     3181 gcgcctccgg gtcccagtag ggtgcaccca ggccggtgaa tgccggcacc atatagacgc
     3241 cgtgctccac acccacttcc tgcgccatgg cctcggtctc gcgggcgtga gagatcagcg
     3301 caatgccgtc ccgcagccac tggatcgcgg caccggcaat gaaaatgctg ccttccaggc
     3361 cgtaggtcac ctgccctttc aggcgataac ccaccgtggt cagcaggcgg ttgcgcgact
     3421 gcagcgcctc gctgccggta ttcaggatca tgaagcagcc ggtgccatag gtgctcttga
     3481 tcatgccggg tttgaaacag gcctggccga tcagcgccgc ctgctggtcc cccgccatgc
     3541 cggcgacggg aatcgccccg ccaaacaggg acggatcggt ggaaccgaaa tccgccgcgc
     3601 agtcctccac gtcaggcagc agggctttgg gcacattgaa cagactcagc agctcgtcgt
     3661 cccactgctg ggtgtggatg ttgaacagca gcgtacgcga cgcattggtg gcatcggtct
     3721 tgtggctttt gccgccggtc agccgccaca gcaggaaggt atcgatggtg ccaaaggcca
     3781 gctcaccctg ctccgcccgt gcccgcgcgc cgtccacatg gtccagaatc caggcaatct
     3841 tggtggcgga aaaatagggg tcgatcagca ggccggtctt ctcctgtacc agggcctcat
     3901 ggccgttgga ccctttaccg ccggctcctt ttccacctga tcggaggctg acgcaggttt
     3961 ccgccgtgcg ccggtcctgc cagacgatgg cgcggtggac cggtttgccg ctggcgcggt
     4021 cccacaccac ggtggtttca cgctggttgg tgatgccgat accggcgatg tccgccgcgc
     4081 tcagctgggc att
//
LOCUS       DASUEG010000572         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1727585, whole genome shotgun sequence.
ACCESSION   DASUEG010000572 DASUEG010000000
VERSION     DASUEG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727585"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..530)
                     /locus_tag="VFX11_10735"
     CDS             complement(24..530)
                     /locus_tag="VFX11_10735"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX4939143.1"
                     /translation="MKISLQQDYPASLSTLWRVFGNPDYPHRKYRALGITGYEIHRFD
                     ASADHIDLDMSRVIAIPEDRIPALVQRFLHPEHTLRYVSSWHLRSSTVADFDLSILPH
                     ALPVHITAKGSLTEQDAKHSHMSLDFDIKAHVPLLGGKIEKLIGQQLEKSFQSDHAFT
                     LRYLAENA"
     gene            complement(660..833)
                     /locus_tag="VFX11_10740"
     CDS             complement(660..833)
                     /locus_tag="VFX11_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939144.1"
                     /translation="MGDVVPFRKPAAKPKGMCQHGFHQWKIWQEKQFDVQQGKLVTVY
                     RCERCGEQKVKAL"
     gene            complement(835..1986)
                     /locus_tag="VFX11_10745"
     CDS             complement(835..1986)
                     /locus_tag="VFX11_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4939145.1"
                     /translation="MSVQYPAVDPEGLLEYSVVFTDRALNHMSKVFQQVMRDISSTLK
                     QVYNADAIAIVPGGGSFGMEAVARQLATGQKCLVLRNGWFSYRWTQILDIGHIPSESI
                     VLKAHPVESGRQVAYAPAPVDEVVATILREKPAVVFAPHVETASGIILPDDYLRAVGQ
                     AVHDVGGLFVLDCVASGAIWVDMKACGVDVLISAPQKGWSASPCAALILMSQAARNRV
                     EATTSSSFAADLKKWLHIMHAYESGGHMYHATMPTTALAAFRNAMQETVSTGLELLRT
                     QQWELGTQVRALMARKGFRSVAAPGFEAPGVVVCYTDDDQIHNGRKFIEQGLQTASGV
                     PLMCDEAADFKTFRVGLFGLDKLNDTARAVDNLDRALTRILAGQPTPVA"
     gene            complement(2087..>3083)
                     /locus_tag="VFX11_10750"
     CDS             complement(2087..>3083)
                     /locus_tag="VFX11_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939146.1"
                     /translation="LPTLARNLARDHAQWGFYQRLAETHDPQRMFARPRQRVEVRHHK
                     PGLLDFRPPEGRVEILSFESSFQTVNPAMRDAYARHRRNRIAWAEHWRHDDGPRPTIC
                     LIHGFVADPYWINSRFLALPWFYRQGYDVLLYTLPFHGRRQERTSPFSGHGYFSHGLS
                     HLNETIAHAVHDFRLFLDYLEDIGSPGFGVTGISLGGFTTALLAATEDRLAFAIPNVP
                     LASIVDLILEWSPASEILRTTMRLSDTSLREVRHASAAACPLTYAPKLAHDRLMIVAG
                     AGDRFASPKHSRLIWEHWDHPLLHWFPGNHLIHLDQGRYLREMAAFMRHIGFDRG"
BASE COUNT          606 a          857 c          966 g          654 t
ORIGIN      
        1 ttgtcttttc tgacgtaatg ctctcaggcg ttttcggcga gatagcgcaa ggtgaacgca
       61 tgatcagact ggaaggattt ttcgagctgc tgtccgatca gcttttctat tttccccccc
      121 aacagcggga catgtgcctt gatgtcgaaa tccagggaca tgtgtgagtg cttggcatcc
      181 tgctcggtca gtgagccctt cgctgtaatg tgcactggta gcgcatgcgg caggatgctg
      241 aggtcgaagt cggccaccgt tgatgatcgc aagtgccatg acgatacata gcgaagggtg
      301 tgctcgggat gcaggaagcg ctgcaccagt gcaggaatcc tgtcttcagg aatggcgatg
      361 acgcggctca tgtccagatc gatatgatcg gcagaggcat caaaacggtg gatttcgtaa
      421 cccgtgatgc ccagcgcccg gtatttgcgg tgcggatagt cggggttgcc gaatacccgc
      481 cacagggtgc tgagactggc gggataatct tgctgcaggg agattttcat cgggagacaa
      541 cgtcaatcag ggagaattga tgtgtctagc atatcgtgat tttccgctgc agtaatgctg
      601 cgcggtcgtc ctgcggccat gaacggatga ccgcagggtg ctcctttctg tcaggctggc
      661 tagagggctt tcaccttctg ttcgccgcat cgctcacaac ggtaaaccgt caccagtttt
      721 ccttgctgaa cgtcgaactg cttttcctgc cagatcttcc actggtgaaa cccgtgctga
      781 cacatgccct tgggctttgc cgccggcttg cggaaaggaa caacgtcgcc catgtcatgc
      841 caccggcgtc ggctggcctg caagaatgcg ggtcagcgcg cgatcaaggt tgtcgacggc
      901 gcgtgcggtg tcattcagct tgtcgagtcc gaacaggccg acacggaatg tcttgaagtc
      961 agccgcctcg tcacacatca atggaacgcc cgatgcggtt tgcagtccct gctcgatgaa
     1021 cttgcggccg ttatgaatct ggtcgtcatc ggtgtagcac accacgacgc cgggcgcttc
     1081 aaagcccggg gcggcgacac tgcggaatcc cttgcgtgcc atcaaggcac gcacctgggt
     1141 accgagctcc cattgctggg tgcgcaacaa ttccagtcct gtcgacacgg tttcctgcat
     1201 ggcattgcgg aaggctgcga gtgctgtggt cggcatggtc gcgtgataca tgtgcccgcc
     1261 actttcgtaa gcgtgcatga tgtgcagcca ttttttcagg tctgcggcaa agctgctgct
     1321 ggttgtcgct tccacacggt tgcgggcggc ctggctcatc aggatgaggg ccgcgcatgg
     1381 tgatgcactc cagcctttct gcggcgcgct gatcagcaca tcgactccgc aggctttcat
     1441 gtccacccag atggcgcccg acgccacaca gtccagtacg aacaggccgc caacgtcatg
     1501 cacggcttgt ccgactgccc gcagataatc gtccggcagg atgatgccgg aggcagtctc
     1561 tacatgcgga gcaaatacta cggcgggttt ttcgcgcagg atggtcgcca ccacctcgtc
     1621 aaccggtgcc ggggcatagg cgacctggcg accggattcc accgggtgtg ccttcagcac
     1681 aatgctttcc gacgggatgt gtccgatgtc gagaatctgt gtccaccggt agctgaacca
     1741 gccgttgcgc agaacgaggc atttctggcc ggtggccagc tgtcgtgcca ctgcttccat
     1801 gccaaagctg ccaccccccg gcacgatggc gatggcgtcc gcgttgtaaa cctgcttcag
     1861 ggtggacgag atgtcccgca tgacttgttg gaacactttg gacatgtggt tcagtgcgcg
     1921 atcggtaaag accacggagt attcgagcag accttcggga tcaacggcag ggtactggac
     1981 agacatggga acttccttgc ggctgtgcag gcagaggcag cagtggtctg ctgcttgttg
     2041 ttttccgcat catgcgggga tgcctgtcaa tctgcaagct gacgactcag cccctgtcaa
     2101 aaccgatatg gcgcatgaag gcagccattt cacgcaggta gcgcccttgg tccagatgga
     2161 tgaggtggtt tcccggaaac cagtgcagga gagggtggtc ccagtgttcc cagatcagtc
     2221 gcgaatgctt gggtgatgca aagcggtcac cggcgccggc aacgatcatc aggcgatcat
     2281 gtgccagttt cggtgcatag gtcagcgggc atgctgctgc actcgcatgg cgaacctccc
     2341 gtagcgaggt gtcactcagg cgcatggtgg tgcgcaggat ttcactggca gggctccact
     2401 ccagaatcag gtcgacaatg ctggccagcg gtacattggg aatggcaaag gcgaggcggt
     2461 cttccgttgc ggccagcaag gcagtggtga atccaccgag cgaaatgccg gtcactccga
     2521 atccggggct gccaatgtct tcaaggtaat ccagaaacag ccggaaatcg tgcacggcgt
     2581 gggcgatggt ttcgttcagg tgtgacaagc catggctgaa atagccatgg cccgagaacg
     2641 gcgatgtccg ctcctgccgg cggccatgga agggcagcgt gtagagcaat acgtcgtagc
     2701 cctgccggta gaaccatggc agtgccagaa accggctgtt gatccagtaa gggtcggcta
     2761 caaagccgtg aatcaggcag atggtgggac gaggtccgtc atcatgtcgc cagtgctctg
     2821 cccaggcgat gcggttgcgc cggtgccgtg cataggcatc acgcatggct ggattaaccg
     2881 tctggaacga gctttcgaac gacaggattt cgacccggcc ctcaggcggg cggaaatcca
     2941 gcagacccgg cttgtgatgc ctgacctcta cacgctgccg cggccgggca aacatgcgtt
     3001 gcggatcatg ggtttctgcc aggcgctgat agaagcccca ctgggcgtga tccctggcca
     3061 gattgcgggc gagggtgggc agg
//
LOCUS       DASUEG010000573         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1021897, whole genome shotgun sequence.
ACCESSION   DASUEG010000573 DASUEG010000000
VERSION     DASUEG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021897"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /locus_tag="VFX11_10755"
     CDS             complement(<1..2196)
                     /locus_tag="VFX11_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4939147.1"
                     /translation="MTSTHNITLLLQPHFEQGLELWLEGGMLRFKAPKEVLTPTLMGL
                     LKQNKEAILSWLQSEQNKKAEPKIVDEYPLAFTQGAIWMLYRFAPNSPAYNTTFACVL
                     NGAVNETAVRQAFHALLIRHPVLRSTFADTDNGPRQRVWDHIEMPLQFIDGSQWDEQQ
                     LDAQLNQEADAPFDLQKEPCLRVKIVRNSVRGDILIATIQHVGADLWALLIVAKDIKD
                     YYQRAARGEVLSLEPLAATYRHHVEWQQQFMDSERGKKERRYWQRSLRGAPMTVSLPT
                     DFPRPPVLLLQSKVIKQEVEAERYRAIRAYCKSHSITPFVFVQAALQLLVTQRTGAQD
                     FLIGTPTMGRSRKGMDQVVGDFANPVVLRAQVQPHATLTSLFQQVKRTLLSAMEHQEY
                     PFPSVVQDCNPPRDSSRTPLFQLMFVWHQGNAEMAQDDWIKSVLPMSGPRGAPYDVML
                     AVSDLGDRFELNWTYQTSLYRPETVARMGQQVQRFMQQFLQADGNQSLADLVAAIAED
                     AKQGGADRILPQPQRNLAMESALLEQIPALIGQEFCVIQQTLHGEATQTPIELQRLFI
                     GCGDERRRFDWQLELQQQIQPGQLDDIVLYPQLPRTATGTIDIAQLSQRPLVDHTAVQ
                     ARLRDAGYAADDMLVLRNLVHRPTWDAEELAPAKKSTAKRAALRALDISQRPDAWATG
                     APLPPTAVTSSHLFEALQKTAQRYPDRGLTLLDNDLRESRYSYAQLVDDA"
BASE COUNT          422 a          680 c          697 g          480 t
ORIGIN      
        1 ggcgtcatcc accagctggg cataactgta acgggattcg cgcagatcgt tgtcgagcag
       61 cgtgaggcca cgatcgggat aacgctgggc cgttttctgc agcgcttcga acaggtggga
      121 agaggtgact gccgttggcg gcagtggcgc gccggtcgcc caggcatcgg gacgctggct
      181 gatatcgagt gcacgcaatg cagcacgctt tgccgtcgat tttttcgcgg gcgccagttc
      241 ttcggcatcc caggttggac gatggaccag gttgcgcagg accagcatat catcggcagc
      301 gtagcccgca tcacgcaggc gtgcctgcac tgctgtgtga tcaaccagcg ggcgctggga
      361 caactgcgcg atgtcgatgg tgccagtcgc ggtgcgcggc agttgcggat acagcacgat
      421 atcgtccagc tgtcctggct ggatctgttg ctgcaattcc agctgccagt cgaagcggcg
      481 acgctcatcg ccgcaaccaa tgaacaggcg ctgtaactcg attggtgttt gggttgcctc
      541 gccgtgcagg gtttgttgaa ttacgcagaa ttcctgtccg atcagggccg ggatctgttc
      601 cagcagtgcg gactccattg caagattacg ctgcggctgc ggcaggatgc gatccgctcc
      661 gccttgcttc gcatcttctg cgattgccgc caccagatcc gccagcgact ggttaccatc
      721 cgcctgcaga aactgctgca tgaaccgctg cacctgctgg cccatgcgcg ccacggtttc
      781 gggacggtac aaactggtct ggtaggtcca gttcagttca aaacggtcgc ccagatcgct
      841 taccgccagc atcacatcat aaggcgcacc gcgcgggccg gacatgggca gcactgactt
      901 gatccagtcg tcctgcgcca tttcggcatt gccctggtgc catacgaaca tcagctggaa
      961 cagcggcgtg cgactgctgt cacggggcgg attgcaatcc tgcaccaccg atggaaatgg
     1021 atattcctga tgctccattg ccgacagcag tgtgcgcttc acctgctgga acaggctggt
     1081 cagagtcgcg tgcggctgca cctgtgctcg cagcacgacc ggattggcga aatcacctac
     1141 cacctggtcc atgcccttgc gggagcgtcc catggtgggg gtgccgatca gaaaatcctg
     1201 cgcgccggtg cgctgggtca ccagcagttg cagcgcggcc tgcacaaata caaagggcgt
     1261 gatgctgtgg cttttgcagt acgcgcggat ggcacggtag cgctccgcct ccacttcctg
     1321 cttgatgact ttgctttgca acagcaacac cggcgggcgg ggaaaatccg ttggcaggct
     1381 caccgtcatc ggcgcgcccc gcagcgaacg ctgccagtac cggcgttcct ttttgccgcg
     1441 ctcgctgtcc atgaactgct gttgccactc gacatggtgg cgataggtcg cggccagcgg
     1501 ttccagcgac agtacttcgc cacgcgcggc acgctggtaa taatccttga tgtccttggc
     1561 gacgatcagc agcgcccaca ggtcggcgcc cacatgctgg atggtggcaa tcaggatgtc
     1621 gccgcgcacg ctgttgcgca cgatcttcac ccgcagacag ggttctttct gcagatcgaa
     1681 cggcgcatcc gcttcctggt tcagctgcgc atccagctgc tgttcatccc actggctgcc
     1741 atcgataaac tgcagcggca tctcgatgtg atcccacacc cgctgacgcg gaccgttgtc
     1801 ggtatcggcg aaagtactgc gcagaacggg atggcggatc agcaaggcat ggaaggcctg
     1861 gcgcacggcc gtttcattga ccgcaccgtt gagcacgcag gcaaaggtag tgttgtaggc
     1921 cgggctgttc ggcgcgaacc ggtacagcat ccagatggcg ccctgagtga acgccagcgg
     1981 atattcatcg acgattttcg gttcggcttt tttgttctgc tctgattgca gccaggagag
     2041 gatggcttcc ttgttttgct ttagcaggcc catcagtgtg ggggtcagca cttctttcgg
     2101 cgccttgaag cgcagcatgc cgccttccag ccacaactcc agcccctgtt caaagtgcgg
     2161 ttgcagcagc agcgtgatgt tgtgggtgga cgtcatagaa tacctgtcgc tcaattcaat
     2221 gccagaaggg aagtggagcc tgtccgcaga tcaagacacg cggtgaatac atccctgta
//
LOCUS       DASUEG010000574         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_842156, whole genome shotgun sequence.
ACCESSION   DASUEG010000574 DASUEG010000000
VERSION     DASUEG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842156"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFX11_10760"
     CDS             <1..918
                     /locus_tag="VFX11_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939148.1"
                     /translation="YDLNEQTYLRAGRFVAPTFMISESRMIGYAQPAVRPVAEVYLLS
                     PISYMNGVDGGYKFLLGDTLVKLRAGVGTLNQEINQVNGTLNFKFDIKSFDTTVEHGA
                     HAFRVGYQKISMDVMNDALELYDLAMDQLEDNGVANASSIHDSMQRLDVPLDFISLGY
                     MYDDGGMFVQTEYAVRKFDSDFVQSLDGFYLLGGYHFGKWSPYVSFSKLIHQDQAEFP
                     ALDTASIDPGLGGLVSAVNAGSQLLIERDAWTVGVRWDLLSGVALKAQIDHIIKPKNT
                     VAEFVNATNEFFAEERDINVVSAAIDFAF"
     gene            933..1370
                     /locus_tag="VFX11_10765"
     CDS             933..1370
                     /locus_tag="VFX11_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4939149.1"
                     /translation="MKRNLKSVTAVLIGLGALLALGTVNAEMVVIVSDKNPTATVSQA
                     DVEKIFLGKMKLFPDGSSAIPLDLPEKSPEKNNFYEKALSKTDSQLRAYWSRIIFTGA
                     GAPPKAVESSSDMVKLVAENPNTIGYVDKSLLAPGVKVVWQVP"
     gene            1477..>2457
                     /locus_tag="VFX11_10770"
     CDS             1477..>2457
                     /locus_tag="VFX11_10770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939150.1"
                     /translation="MLEAVNRILNQATPATRCLITVAILVPFYLLYFATQAWALGDAD
                     IRAVIRPGVVMAMQILVVAVTLMTLAVGIWCWPLRRSPLPVPHLYTFLACTSTLLYTG
                     EAIMQGNLTSPANMVIIGNVVIGLMLLDQRAVLLAFIQAVVTLVLNDVLVLLEIVPYA
                     PMLTPAAFNQGEPQEWFSIWRNMVLIFGLAGTCFFGVLLFNQFEFQRAALRRQSYTDV
                     LTGLANRRFFMERLQAETRRAECMQRPFCLALLDVDHFKQVNDRFGHPVGDDVLRVIA
                     ATMKASMRCPTDLPARVGGEEFAILLADTDLNGAQVACERLASCLSQQEFK"
BASE COUNT          496 a          691 c          746 g          524 t
ORIGIN      
        1 tacgacctca acgaacaaac gtatctgcgg gccggccggt ttgtcgcacc caccttcatg
       61 atctcggaat cgcgcatgat cggttacgcc cagccagcgg tgcggccggt ggcggaagtg
      121 tatctgctga gccccatttc ctacatgaat ggcgtcgacg gcggctacaa attcctgctg
      181 ggggacacgc tggtgaaact gcgcgccggc gtgggcacgc tgaatcagga gatcaaccag
      241 gtcaacggca cgctgaattt caagttcgac atcaaatcct tcgacaccac cgtcgaacac
      301 ggtgcgcacg cgttccgggt gggctaccag aaaatcagca tggacgtgat gaacgacgcg
      361 ctggaactgt atgacctggc catggaccag ttggaagaca acggcgtcgc gaacgcctcc
      421 agtattcacg acagcatgca gcgcctggac gtgccactgg atttcatcag cctgggctac
      481 atgtatgacg acggtggaat gttcgtgcag accgagtatg cggtgcgcaa atttgattcc
      541 gacttcgtgc agagccttga tggattttat ctgctgggtg gctaccactt cggcaaatgg
      601 tcgccctacg tttcattcag caagctgata catcaggatc aggcggaatt tccggccctc
      661 gatacagcct ccatcgaccc gggcctgggc ggactggtgt cggcggtgaa tgccggttcc
      721 cagctgttga tcgaacggga cgcctggacg gtgggagtgc gctgggatct gctcagcggt
      781 gtcgcgctga aggcgcagat cgatcacatc atcaagccga agaacaccgt tgcggaattc
      841 gtgaacgcca caaacgaatt tttcgccgag gaacgtgaca tcaatgtggt ttccgccgca
      901 atcgactttg cgttctgata ggagggcgag acatgaagcg taacttgaaa agtgtgactg
      961 cagttctgat cggtttgggc gccctgctgg ccctgggcac ggtaaatgct gaaatggtgg
     1021 tgatcgtcag cgacaaaaat ccaaccgcga ccgtcagcca ggcagatgtg gagaaaatat
     1081 ttctgggcaa aatgaagctg ttccctgatg gcagcagtgc gataccgctg gacctgccgg
     1141 aaaagagccc ggagaaaaac aatttttatg aaaaagcctt gagtaaaacg gattcgcagc
     1201 tgcgggccta ctggtcgcgg atcatcttca ccggcgccgg tgcgccgccc aaggcggtgg
     1261 aatcatcttc cgacatggtc aagctggtgg cagaaaatcc gaataccatc ggctatgtgg
     1321 acaagtcgtt actggcaccg ggtgtaaaag tggtgtggca agtaccctga ttgccgccgc
     1381 ctgcaacagc agggcgttct gccacatggg aacgtcctgc cttcattttg ccgccaggtt
     1441 ttggcaatcc cacggaatca cgatgtttga tcgaccatgc ttgaagctgt caaccggata
     1501 ctgaatcagg cgacaccggc cacgcgctgc ctcattaccg tggccattct ggtaccgttc
     1561 tacctgttgt atttcgcaac ccaggcctgg gccctgggcg acgccgatat tcgcgccgtg
     1621 atccgccccg gcgtggtgat ggcaatgcag atcctcgtgg tggcggtgac gctgatgacg
     1681 ctggccgtgg gcatctggtg ctggcctctg cggcgctcgc cgctaccggt gccgcacctg
     1741 tacaccttcc tggcctgcac ctcgacgttg ctctacaccg gtgaagccat catgcaaggc
     1801 aatctgacgt cgccggcaaa catggtgatc atcggcaatg tggtgatcgg gctgatgctg
     1861 ctcgaccagc gcgcagtgct gctcgcgttt atccaggcgg tggtgacgct ggtgctgaac
     1921 gatgtcctgg tgttgctgga gatcgtaccc tacgcgccca tgctcacgcc ggccgcgttc
     1981 aatcagggcg agccgcagga atggttcagc atctggcgca acatggtgct gatatttggt
     2041 ctggcgggta cctgcttttt cggtgttctg ctattcaacc agtttgaatt ccagcgtgcg
     2101 gctctgcgca ggcagtccta caccgacgtg ctgaccggtc tcgccaaccg gcgttttttc
     2161 atggaacgcc tgcaggcgga aacgcgccgc gccgaatgca tgcagcgtcc attctgcctg
     2221 gcgttgctgg atgtggatca cttcaagcag gtgaacgacc ggttcggcca tccggtcggt
     2281 gacgacgtgt tgcgcgtcat cgccgccacg atgaaggcga gcatgcgctg ccccaccgat
     2341 ctgcccgccc gcgtgggtgg tgaggaattc gcgatcctgc tggcggacac ggatctgaat
     2401 ggcgcacagg tggcatgcga gcgtcttgcg tcctgcttga gtcagcagga attcaag
//
LOCUS       DASUEG010000575         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1174972, whole genome shotgun sequence.
ACCESSION   DASUEG010000575 DASUEG010000000
VERSION     DASUEG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174972"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX11_10775"
     CDS             complement(<1..457)
                     /locus_tag="VFX11_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4939151.1"
                     /translation="MTAARHTGLITLSIMMATIMQALDTTIANVALPHMQGSLNATQE
                     QASWVLTSYIVACAIMTAPTGILAARIGRKRLFLISVAGFTFTSVLCGVAQNLPEMVL
                     FRLLQGVFGAGLVPLSQAVLLDTYPKEKHGQAMSMWGMGVMPGPILGPSL"
     gene            complement(459..1592)
                     /locus_tag="VFX11_10780"
     CDS             complement(459..1592)
                     /locus_tag="VFX11_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX4939152.1"
                     /translation="MNSTSVTNANNTQPRSNRLRHSLMALGIVLVAGGGLWSWLTSGR
                     YVSTDNAYIKAEKILLAPDVSGNVVAVLVKDNQPVHKGDELFRIDPAPYRIAADRAQA
                     DLSTSVLRINELKALYAQKQTDLERARVEAQFAERDLKRQLALIAKGSVSESQRDDAE
                     LRHDQSRKDVLKLQHEVTEILASLANKPDIAVAEHPLFIAAQTAVAKANLDLARTSVR
                     APADGIVGSAPSVGEYARAGLPLVNFIGTGQVWVEANFKETDLTDIKPGQTVSIHVDS
                     YPGQTWTGKVESISPASGSEFSILPAQNSTGNWVKVVQRIAARISVDDGPPDLTLRAG
                     MSTEVEIDTGRYPHLGQPPHVTQQPQQKQPPLATQQPDTLQDS"
     gene            complement(1611..>2080)
                     /locus_tag="VFX11_10785"
     CDS             complement(1611..>2080)
                     /locus_tag="VFX11_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX4939153.1"
                     /translation="EPAVSTTDAPIKKPQDALGFVLSDVARLIRRNFNRQVQKLGLTQ
                     AQWQVLAFLARNEGVRQIQLADSLDMQPISVARIIDRMQASGWVERRPDPTDRRAFNL
                     YLTAQANPILEQMWDQGAITRAEALKNIPAADQDQLLRTLLRMRENLGHDTGA"
BASE COUNT          312 a          648 c          706 g          414 t
ORIGIN      
        1 ccagactggg gcctagaatc ggcccgggca tgacgcccat gccccacatc gacatggcct
       61 ggccatgttt ttccttcgga taggtatcca gcagaacggc ctgcgacagc ggtaccagtc
      121 cggcgccgaa tacgccctgc agcaggcgga acagcaccat ctccggcagg ttctgcgcca
      181 cgccgcacag cactgatgtg aaggtgaagc cggcgacgct gatcaggaac agccgcttgc
      241 ggccgatgcg ggcggcgagg atgccggtgg gcgcggtcat gatggcgcag gcgacgatat
      301 aggacgtgag cacccacgac gcctgctcct gcgtcgcgtt gagcgagccc tgcatgtgcg
      361 gcagggccac gttggcaatg gtggtgtcca gtgcctgcat gatggtggcc atcatgatcg
      421 acagggtgat caaccccgtg tgccgggccg ccgtcatatc aggaatcctg cagcgtgtcc
      481 ggctgttgcg tggctagcgg tggctgcttc tgctgcggtt gctgggtgac gtgtggtggc
      541 tgccccaggt gcggatagcg tcccgtgtcg atctccactt ccgtgctcat gccggcgcgc
      601 agggtcagat ccggcggccc gtcatccacg ctgatgcggg cggcgatacg ctgtaccacc
      661 ttcacccagt tgccggtgga attctgcgcc ggcaggatgg aaaactccga accgctggcg
      721 gggctgatgc tttccacctt gccggtccag gtctggccgg ggtagctgtc gacgtggatc
      781 gacacggtct ggcccggctt gatgtcggtc agatcggttt ccttgaaatt ggcctcgacc
      841 cacacctggc cggtgccaat gaaattcacc aggggcaggc cggcgcgggc atattccccc
      901 acgctgggcg cgctgccgac gatgccgtct gccggtgccc gcaccgacgt gcgcgccaga
      961 tccagattgg ccttcgccac agccgtctgc gcggcgatga acagcggatg ctccgccacg
     1021 gcgatgtcgg gcttgttggc gagggacgcc agtatctcgg tgacttcgtg ctgcagtttc
     1081 agcacgtcct tgcgggattg gtcgtggcgc agttcggcgt cgtcacgctg ggattccgac
     1141 accgagcctt tcgcaatcag cgccagctgc cgcttcaggt cgcgctcggc aaactgcgct
     1201 tccacccggg cccgttccag atccgtctgc ttctgcgcat acagggcttt cagttcgttg
     1261 atgcgcagca cgctggtgga caggtcggcc tgcgcccggt ctgccgcaat gcggtagggc
     1321 gcggggtcga tgcggaacag ttcatcgccc ttgtgcaccg gctggttgtc tttcaccagc
     1381 acggccacca cgttgccgga tacgtccggt gccagcagga tcttttccgc cttgatgtag
     1441 gcgttgtcgg tgctgacgta acggccgctg gtgagccagg accacaggcc gccgcccgcc
     1501 accaggacga tgcccagggc catcagactg tgccgcagcc ggttggatcg gggttgggta
     1561 ttgtttgcat tggtcacgct ggtgctgttc atgctgctag cctcgtacta tcaggccccg
     1621 gtgtcgtggc cgaggttttc ccgcatcctt agcaaggtgc gcagcagctg gtcctggtct
     1681 gccgccggaa tgtttttcag ggcttccgcc cgggtgatgg cgccctggtc ccacatctgc
     1741 tccaggatcg ggttcgcctg ggccgtgaga tagaggttga aggcacggcg atcggtggga
     1801 tcgggccggc gttccaccca gccggacgcc tgcatgcggt cgatgatgcg ggccacgctg
     1861 atgggctgca tgtcgaggct gtcggccagc tggatctggc ggacgccctc gttgcgggcc
     1921 agaaacgcca gcacctgcca ctgggcctgg gtgaggccca gcttctgcac ttgccggttg
     1981 aaattgcgcc gtatcagacg ggccacgtca ctgaggacga agccgagggc atcctggggt
     2041 ttcttgatgg gggcatcggt ggtgctcacg gcaggctcca
//
LOCUS       DASUEG010000576         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1916845, whole genome shotgun sequence.
ACCESSION   DASUEG010000576 DASUEG010000000
VERSION     DASUEG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916845"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFX11_10790"
     CDS             complement(<1..506)
                     /locus_tag="VFX11_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX4939154.1"
                     /translation="MSIPQKIVDRLNAHGISYQVVEEESISPLHPLLSGDQIPRGQIV
                     QQVVLEDSLGRVQVLLPADRLLDLQKLCEDKGRNLTAISQDTSRLLASSLGMETLPAI
                     PLNDNLPLIIDRALLKPVALYLEVSQSGQYLTLPQAEFAKLVANALSGDFSVPLTRLS
                     QDPNDRAAV"
     gene            complement(612..1259)
                     /gene="rsmD"
                     /locus_tag="VFX11_10795"
     CDS             complement(612..1259)
                     /gene="rsmD"
                     /locus_tag="VFX11_10795"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX4939155.1"
                     /translation="MRKSTKPAGKAGAGPKGPGEVRIIGGQWRGRRLQFSAHEGLRPT
                     LDRYRETLFNWLMFDVEGARCLDLFAGSGALGFEALSRGAREVDFVDANPRVCSDIRA
                     NLGKLGSEKGRVWHSAAEAWLKARRNDLASGTIAPYDLVFLDPPFHQDLLQACIHLLE
                     TGGWLAENARIYLEAENSFDRSKLPYHWRVHKEKDGGNKIFLLLIRQPDHPLAPA"
     gene            complement(1267..>2747)
                     /locus_tag="VFX11_10800"
     CDS             complement(1267..>2747)
                     /locus_tag="VFX11_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4939156.1"
                     /translation="LVSALLLLVGAVIIVTAGYMVTPLLTKEGESRPAPKVTVSNSSA
                     AAREKVNGLPLTSPEIIERTPAKAHRIHIQSWQTPKGATVMFVESPEIPMLDVRLVFN
                     AGSARDGDLPGLANLTNGMLSEGAGVADVDQIARHFEGLGARLDNGAYRDMAVVSLRT
                     LSDPQYREPALSLFSDVVAHPTFPEDSLERLRNQLLLSLQYEKQDPGSQVSKAFFQQL
                     YPNAPYGTPTSGTEESLPRVTVTEMKAFHQRYYVSRNLVVAMIGAISRQEAEKIARQL
                     DAQLPEGSAAPALPAPAALAKAVEHHIEFPSSQTHILAGSLGVKRGDPDWYPLYVGNE
                     ILGGSGFASRLNQVIRQDNGLAYSVYSQFAPMAVAGPFMMGLQTRNEQATQALELLDK
                     TVREFIEKGPSDKDLADAKRNLLNSFPLQTASNGSIVDYLGMIGFYKLPLDYLERYPE
                     QVEKVTADDIREAFARIVQADKLLTVTVGATQAAPAAATAKP"
BASE COUNT          437 a          811 c          966 g          533 t
ORIGIN      
        1 acggcggcgc ggtcgttggg atcctggctc aggcgggtca ggggcacgct gaaatcaccg
       61 ctcagggcat tggccaccag cttggcgaac tcggcctggg gcagcgtcag gtactggccg
      121 ctctggctca cttccagata cagagcgacg ggcttgagca gggcgcggtc gatgatcagg
      181 ggcaggttgt cgttgagcgg aatcgccggc agggtttcca tgcccagcga actggccagc
      241 aggcggctgg tgtcctggct gatggcggtg aggttgcggc ccttgtcctc gcacagtttc
      301 tgcagatcca gcaggcggtc ggcgggcagc agcacctgga cccggccgag ggaatcctcc
      361 agcaccacct gctgcacaat ctggccgcgg gggatctggt cgccgctgag caacgggtgc
      421 aggggcgaaa tgctttcctc ttcaacaacc tggtaggaaa tgccgtgcgc gttcagacga
      481 tccacgattt tctgcggaat ggacatactg ccgatcctcg ggtgtgattc cactaagcat
      541 agtgaccgcc ccgggaagct gcaaagatgc gctcctgagg cggtttcggc aaagcggccg
      601 caggtggttg atcaggctgg ggcgaggggg tgatccggct ggcggatgag cagtaaaaaa
      661 atcttgttgc cgccgtcctt ttccttgtgg acgcgccagt ggtagggcag tttgctgcgg
      721 tcgaagctgt tttccgcctc caggtagatc ctggcgtttt ccgccagcca gccgccggtt
      781 tccagaagat gaatgcaggc ttgcagaaga tcctggtgaa aaggcggatc gaggaagacc
      841 aggtcgtagg gggcgatggt gccgctggca aggtcgttac ggcgggcctt cagccaggct
      901 tcggcggcgc tgtgccagac gcggcctttc tcgctgccca gtttgcccag gttggcgcgg
      961 atgtcgctgc agacgcgggg attggcgtcg acgaaatcca cttcccgcgc gccccgggac
     1021 agtgcctcga agccgagggc gccgctgccg gcaaacaggt ccaggcagcg ggcgccttcc
     1081 acgtcgaaca tcagccagtt gaacagggtt tcccggtaac ggtcgagggt ggggcgcaaa
     1141 ccttcgtggg cgctgaactg caggcggcgg ccacgccatt ggccgccgat gatgcggact
     1201 tcgccggggc ctttcgggcc ggcgccggct ttgcctgcgg gtttggtgga ttttctcatc
     1261 gcgcgctcag ggcttcgcgg tggcggcagc gggagcagcc tgggttgccc ctactgtcac
     1321 cgtcagcagc ttgtctgcct gcacgatacg ggcgaaggct tcgcgaatat cgtctgctgt
     1381 cactttctcc acctgctccg ggtagcgctc caggtaatcc agcggcagct tgtagaagcc
     1441 gatcatgccc aggtaatcca cgatgctgcc gttgctggcg gtctgcagcg ggaagctgtt
     1501 gagcaggttg cgcttggcgt cggccagatc cttgtcgctg gggcctttct cgatgaactc
     1561 gcgcacggtc ttgtccagca gttccagggc ctgggtggcc tgctcgttgc gggtctgcaa
     1621 gcccatcatg aaggggccgg ctaccgccat gggcgcgaac tggctgtaca cgctgtaggc
     1681 cagaccgttg tcctgccgga tcacctggtt cagccgggag gcgaagccgc tgccgcccag
     1741 aatttcgttg cccacataga gcggatacca gtccggatcg ccccgcttca cgcccaggct
     1801 gccggccagg atatgggtct ggctggaggg aaattcgata tggtgctcca ctgccttggc
     1861 cagagcggcg ggcgccggca gggcgggggc ggcgctgcct tccggcagtt gcgcatccag
     1921 ttggcgggcg attttctccg cttcctggcg gctgatggcg ccgatcatgg cgaccaccag
     1981 attacggctg acgtaatagc gctggtggaa cgccttcatc tcggtcacgg tcacgcgggg
     2041 caggctttcc tcggtaccgc tggtgggggt gccgtagggc gcgttggggt aaagctgctg
     2101 gaaaaaggcc ttcgacacct gggaacccgg gtcctgtttt tcatactgca gcgacagcag
     2161 cagctggttg cgcaggcgtt ccaggctgtc ctcggggaag gtggggtggg ccaccacgtc
     2221 gctgaacagg ctgagggcgg gttcgcggta ctggggatcg gacagggtgc gcaggctgac
     2281 taccgccatg tcgcggtagg cgccgttgtc gagccgtgcg cccagacctt caaaatggcg
     2341 ggcgatctgg tccacatccg ccacgccggc accctcgctc agcatgccgt tggtgaggtt
     2401 ggccagaccg ggcaggtcgc cgtcccgcgc cgagcctgcg ttgaacacca ggcgcacgtc
     2461 cagcatggga atctcggggg attccacgaa catcacggtt gcgcccttgg gtgtctgcca
     2521 ggactggata tggatgcgat gggccttggc gggggtgcgc tcgatgattt ccggcgaggt
     2581 cagcggcagc ccgttcactt tttcacgggc ggcggcagag ctgttgctta ccgtcacttt
     2641 cggtgccggg cggctctcgc cttccttcgt cagcaggggg gtgaccatgt aaccggcagt
     2701 gacgatgatc acagcgccca ccagtagcag cagggcggag acgaggc
//
LOCUS       DASUEG010000577         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1269033, whole genome shotgun sequence.
ACCESSION   DASUEG010000577 DASUEG010000000
VERSION     DASUEG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269033"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFX11_10805"
     CDS             <1..192
                     /locus_tag="VFX11_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HEX4939157.1"
                     /translation="TSVLQALSVAGGTSTFADYNGIRIIRSENGINKAIGFRYGDIED
                     GEQLEQSIQLQSGDVVVVP"
     gene            189..1382
                     /locus_tag="VFX11_10810"
     CDS             189..1382
                     /locus_tag="VFX11_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939158.1"
                     /translation="MSNRDASIQSRAGWLLVAALMVAAGDVVAAREDVLLNASAGAFY
                     DSNRTLVEGDGNEVNGTHQRARVRYQVATESSRIEGALNVLEQQLPGYEYLENDRLGI
                     SFLGNSELERANLNSSVSVQRDTTLSTEVQAAGVINENKDRLLSTLAGGYGYLLTERD
                     QLNVGVTAEKVDYDDIRSAELAEYLFYGVDTGYTRSFSDTTRLQLSLYESVLDNDESG
                     LTGDTVGFIVNLEQQLTPVWRWKAGLGRRESRYEWERVAGLADGFTDTSRSRVSDFEL
                     AYDGETARMKLFGAYDLVPSSGGSLVARRSAGTTILQSWSAVQNTLLNMRYWEQRSDL
                     EVKSGEDLDSLQFTLEHNWRLMRKMNLVFRYSRFERLLLATDARAESDFVEFEIAWIE
                     DPLSM"
     gene            1410..>2488
                     /locus_tag="VFX11_10815"
     CDS             1410..>2488
                     /locus_tag="VFX11_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX4939159.1"
                     /translation="MNTHIAEADHRLDIGLVWKEVRRRIKPALIAFVVVVVIFGTLAL
                     KLPALYRSSATVLIEQQEIPQDLVRTTVSSFAAQRIQVLIQRAMTFSNLSDIIKKYDL
                     YADLRESDPLEQVVEKMREDIEHRMISAEVVDPRSGRPVEATIAFAISYVNPDATTAQ
                     QVTSEIVSLFLEENLRNRAELAEQAETFLGDELKRLEIRMRQLESALADFKEHNLSSL
                     PEQNTNNLNMIERTEREYDEIRRQIQGVEERKVYLETQLAQIEPYQRVVGDGREAVMT
                     AEGRIRFLQNQYLTLSATYSDSHPDVVKTRRELEESLQSGIRDLDRSVVDQQLKILRH
                     EQNAMKLKYSADHPEVRTLARKIAE"
BASE COUNT          606 a          601 c          760 g          521 t
ORIGIN      
        1 accagcgtat tgcaggcgtt gagtgtcgca ggtggtacgt ccactttcgc cgattacaat
       61 ggcattcgca tcattcgtag cgagaacggc atcaataagg cgatcggatt ccgttatggc
      121 gacattgagg atggtgagca gctggagcag agcatccagc tgcaaagcgg tgacgttgtt
      181 gtagtgccgt gagcaatcgt gatgcaagta tccagagccg cgccggctgg ctgctggtgg
      241 cggcattgat ggtggctgcc ggagatgttg tggccgcgcg tgaggatgtg ttgctgaatg
      301 cctcggcggg tgccttctat gattccaacc gcaccctggt tgagggtgat ggcaatgaag
      361 tgaatggtac ccaccagcgg gcccgggtgc gctaccaggt ggcgacggaa tccagccgta
      421 tcgagggtgc actcaacgtg ctggagcagc agctaccggg atatgagtat ctggagaatg
      481 acaggcttgg aatcagtttc ctcggtaata gcgagctgga gcgtgccaac ctgaattcca
      541 gcgtgagcgt gcagcgtgat acgaccctgt ccaccgaggt gcaggcagcc ggcgtgatca
      601 atgaaaacaa ggatcgacta ctgagtacgc tggcaggtgg atatggctat ttgctgacgg
      661 agcgtgacca gctgaatgtc ggcgttacgg cggagaaagt cgattacgac gatattcgat
      721 cggccgagct ggcggagtat ctgttttatg gtgtcgatac cggttacacc agaagtttca
      781 gcgataccac acgactgcaa ctttcactgt acgaaagtgt tctagacaac gatgaatcag
      841 gcctgacagg agacaccgtc ggatttatcg taaatctgga acagcagctt actccggtat
      901 ggcgctggaa ggccgggctg ggccgtcgtg aatcccgcta tgaatgggaa agggttgctg
      961 gccttgcgga tggcttcact gataccagca gaagcagggt atcggatttt gaactggcct
     1021 acgatggcga gaccgcgcgg atgaaactgt tcggtgcgta cgacctggta ccaagcagcg
     1081 gcggcagcct ggtggcgcgc agaagtgcag gaacgacgat cctgcagtcg tggtcggcag
     1141 tccagaatac attgttgaac atgcggtact gggagcagcg tagcgacctg gaagtcaagt
     1201 ccggggaaga cctggattcc ttgcagttta cgctggagca taactggcgt ctgatgcgga
     1261 agatgaatct ggtgttccgc tattcgcgct ttgagcgact gctgcttgcc accgatgcca
     1321 gggctgaatc ggatttcgtc gaattcgaga ttgcctggat tgaagatcca ttatcgatgt
     1381 agaacacaca aggcgacaaa cacgttttta tgaacaccca tatagcagaa gcagatcacc
     1441 gcctggatat tggcctggtc tggaaagaag ttcgcagaag aatcaaacct gccctgattg
     1501 cgtttgtagt ggtggttgtc attttcggta cgctggctct gaagttgccg gcgctttacc
     1561 ggtcttcggc cacggtgctg atcgagcagc aggaaatacc acaggacttg gtgcggacca
     1621 ctgtcagcag ttttgcggcc cagcgtatcc aggtgctgat ccagcgcgcc atgacattct
     1681 ccaatctgag cgacatcatc aagaaatatg atctctatgc cgacttgcgg gaaagcgatc
     1741 cactggaaca ggtggtcgag aaaatgcggg aagacatcga gcacagaatg atcagtgccg
     1801 aagtggttga tccgcgtagt ggtcgtccgg ttgaggcgac cattgccttc gccatttcct
     1861 atgtcaatcc cgacgccacc acggcgcaac aggttaccag cgagattgtg tcgctgttcc
     1921 tggaggaaaa tttgcgcaac cgggctgaac tggcagagca agccgaaacg tttctgggag
     1981 acgaactgaa acggctggaa atccggatgc gccagctgga aagcgcattg gccgatttca
     2041 aggagcataa tctgtcaagc ttgccagaac agaacaccaa caacctgaac atgattgagc
     2101 gtaccgaacg cgaatatgac gaaatccggc gtcagatcca gggtgtagaa gaacgcaagg
     2161 tctacttgga aacacagttg gcgcagatcg aaccctacca gcgggtggtg ggcgatggca
     2221 gggaagcagt catgacagcc gaaggcagga ttcgcttcct gcagaaccag tacctgacgt
     2281 tgtccgcaac ctattccgac agtcatccgg acgtcgtcaa aacacggcgc gaactggagg
     2341 aatcactaca gtctggaatc agggatctgg atcgcagcgt ggtagatcaa cagctgaaaa
     2401 tactgcggca cgaacagaat gcgatgaaac tgaagtactc agccgaccat cccgaagtca
     2461 ggacgctggc gcgaaagatt gctgaatt
//
LOCUS       DASUEG010000578         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2123010, whole genome shotgun sequence.
ACCESSION   DASUEG010000578 DASUEG010000000
VERSION     DASUEG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123010"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX11_10820"
     CDS             complement(<1..282)
                     /locus_tag="VFX11_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939160.1"
                     /translation="MRKRPRQQRSRQMVETLVEATALCIARHGLDGTTTPLIADIAGV
                     SVGSLYQYFENKEELIEALVERIADDVTRALSAGVRAGKDMPLEELARSA"
     gene            364..1608
                     /locus_tag="VFX11_10825"
     CDS             364..1608
                     /locus_tag="VFX11_10825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4939161.1"
                     /translation="MSVAPASAAPRIPGRARPFEKARIKTPAWQRLLLGRDLSPSRAE
                     YDAVVGACWEGDPAMDTLVDWMYSAGAGTARKLFQQAVTQGVDSIPDAPQPLLDFFRA
                     VETPPVWLEPALIDEGVRFIHRIGLAGPYVLRDLALMGGYLLSGFNHVLVMTGALNKD
                     TSLRIAETGQWWVDCTEHGGMDRFGPGFRSTLHVRLVHAMVRRTLAKNPKWDASQWGV
                     PVNQIDMVATYLGFCVVMLGGLRQLGIPVTARESKAVMHLWAYAGWLMGVDERWLVHT
                     ERDGIVLLAHTFMTQSRPDWTSQELGRALSKEPLTRKYPNFANLRSKLAYHQHLSVSR
                     YFLGKQKMQQLGLPDDILPWFPVLTAVPRCVSYLGQKAVPALRQRQEEEGRAAQVAVL
                     QQMFGDSAAAVGIHRDQNAPAH"
     gene            1621..2961
                     /locus_tag="VFX11_10830"
     CDS             1621..2961
                     /locus_tag="VFX11_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4939162.1"
                     /translation="MIGLLSAQDRALRTQLPGEPGLPLLGHSLIFLYSPVKTALEYYR
                     RFGSVYWINVLGQTGVVLLGPEANQLVLRNQDEAFSSSLGWSFFIGRFFTRGIMLLDF
                     DEHKFHRGIMQAAFKKPMLVQYLRDMNPVIGSGIARWKPGKNFRVLKQIKQLTLDIAT
                     EVFMGEKLGPQADKVNRAFVDCVRAGTALVRYPVPGGRWWKGLQGRKLLEQFFRARIP
                     QKRAQPGTDLFSRLCQAEDENGQRFTDEDIVNHMIFLLMAAHDTSTISLCAIFYYLAK
                     YPQWQERVRAESRALQKDTLDHDDLAKLETLDRVIKEAMRLVSPVHGIPRRTVKEVEF
                     QGHVIPADTFVMITPMVSHHLPDHWLDPEQFDPDRFAPGRSEHKGHPYQYLPFGGGAH
                     MCIGLHFADLQIKSILHQVLLRYRWSVPADYVMPLDLTSLPSPSDQLPVTLERL"
     gene            3045..3917
                     /locus_tag="VFX11_10835"
     CDS             3045..3917
                     /locus_tag="VFX11_10835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939163.1"
                     /translation="MSTLKPRQTVLITGCSSGIGRATALHFQRAGWNVVATMRTPEQE
                     TELNRLNNVLLVALDVQDQASIDNALQQALTHFGQIDVLINNAGYALMGAFENASEEQ
                     IQRQFDTNVFGLMRVARAVLPHFRGRNAGLLINVASMAGRLPIPLMSVYNASKWAVQG
                     FSECLTYELAPFNIRVKIVEPGAVNTAFFGRSGDRQNATGVAAYNEFTNAQFPVIDKT
                     GPGGSTSEDVAATLYRAATDGSATLRYAVGVDAKALLSARKMLPDRVFLPMIGWSLSP
                     AVFNRLGRLFYKPT"
     gene            4057..>4502
                     /locus_tag="VFX11_10840"
     CDS             4057..>4502
                     /locus_tag="VFX11_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939164.1"
                     /translation="MSELFSIRGRIALVTGGTGGIGYMIAQGLVKHGAKVYVASRKAD
                     ACEKTARELSAFGTCIGIPADVSTEAGCAALVNELQQREDKLNILVNNAGVTWGASFD
                     TYPGEAWDKIQNLNVKSAFVMTQKLTPLLSAAGTADDPARVINITSV"
BASE COUNT          830 a         1368 c         1423 g          881 t
ORIGIN      
        1 ggcgctgcgg gccagctctt ccagtggcat gtccttgccg gcgcgcacgc cggcgctcag
       61 agcccgggtc acgtcgtccg cgatccgttc caccagcgcc tcgatcagtt cttccttgtt
      121 ctcgaaatac tggtacagcg aacccacgct gacgccggcg atgtcggcga tcaggggtgt
      181 ggtggtgccg tccagcccgt ggcgggcgat gcagagggcg gtggcttcca ccagggtctc
      241 gaccatctgg cgggaacgtt gctgcctggg gcgtttgcgc atggggtccg ccgggaatac
      301 gaattgaata cgaataaagt ttcgcattag tgtggctgtg aatcaaccag ccaggataat
      361 cccatgtctg ttgcccctgc ttctgccgcc ccccggattc ccggccgggc ccgccccttc
      421 gagaaggccc gtatcaagac gccggcctgg cagcgcctgc tgctggggcg ggatctgtcg
      481 cccagccgcg ccgaatatga cgcggtggtg ggcgcctgct gggaaggcga ccccgcgatg
      541 gacaccctgg tggactggat gtattcggcc ggtgccggca cggcccgcaa gctgttccag
      601 caggcggtca cccagggtgt ggacagcatt cccgacgcgc cccagccgct gctggatttc
      661 ttccgcgccg tggaaacgcc gccggtgtgg ctggaacccg cgctgatcga cgaaggggtc
      721 cgcttcattc accgcattgg tctggccgga ccctatgtgc tgcgggatct ggcgctgatg
      781 ggcggttacc tgctgtcggg cttcaaccat gtgctggtga tgaccggcgc gctgaacaag
      841 gacacctcgc tgcgcatcgc cgagaccggc cagtggtggg tggactgcac cgaacacggc
      901 ggcatggacc gtttcgggcc gggcttccgc tccaccctgc atgtgcggct ggtgcatgcc
      961 atggtgcgcc gaaccctggc gaaaaatccc aagtgggacg cgagccagtg gggtgtgccg
     1021 gtgaaccaga tcgacatggt ggcaacctac ctcggctttt gcgtggtgat gctgggcggc
     1081 ctgcgccagc tgggcattcc ggtgaccgcg cgtgaatcga aagcggtgat gcacctgtgg
     1141 gcctatgccg gctggctgat gggggtggac gagcgctggc tggtgcacac cgagcgcgat
     1201 ggcatcgtgc tgctggcgca tactttcatg acgcagagcc gccccgactg gaccagccag
     1261 gaactgggcc gggcgctgtc gaaggaacca ttgacccgca aatatcccaa cttcgccaat
     1321 ctgcgcagca agctggccta tcaccagcac ttgagcgtga gccgctattt tctcggcaag
     1381 cagaaaatgc agcaactggg cctgcccgac gacatcctgc cctggtttcc cgtcctgacg
     1441 gcggtgccgc gctgcgtgag ctatctggga cagaaagccg tgccggccct gcgccagcgc
     1501 caggaagagg aaggccgcgc cgcccaggtg gcggtgctgc agcagatgtt tggcgattcc
     1561 gccgcggcgg tgggcatcca ccgtgaccag aacgcgccgg cgcactgagg aggggatccc
     1621 atgataggtt tgctgtccgc tcaggatcgc gctctgcgca cccaattgcc cggcgagccc
     1681 ggtttgccgc tgctgggcca cagcttgatt tttctctaca gcccggtgaa aaccgcgctg
     1741 gaatattacc gccgtttcgg ctctgtgtac tggatcaatg tgctgggcca gaccggcgtg
     1801 gtgctgctgg ggcccgaggc caaccagctg gtgctgcgca atcaggacga ggcgttttcc
     1861 agcagtctgg gctggtcgtt tttcattggc cgctttttca cacgcggcat catgctgctg
     1921 gatttcgacg aacacaaatt ccaccgcggc atcatgcagg cggcgttcaa gaaacccatg
     1981 ctggtgcagt acctgcgcga catgaatccg gtcatcggca gcggcatcgc gcgctggaaa
     2041 ccgggcaaaa attttcgggt gctgaagcag atcaagcagc tcacgctgga tatcgccact
     2101 gaagtgttca tgggcgaaaa gctggggccg caggcggaca aggtgaatcg cgcgttcgtc
     2161 gattgcgtgc gcgccggtac cgcgctggtg cgctatcccg tgcctggcgg tcgctggtgg
     2221 aaaggactgc agggacgcaa attgctggag cagttcttcc gcgcgcgcat tccgcaaaaa
     2281 cgtgcgcagc ccggcaccga tctgttcagc cggctgtgtc aggccgaaga cgagaacggc
     2341 cagcgtttca ctgacgagga catcgtcaac cacatgatct tcctgctgat ggcagcgcat
     2401 gacacgtcca ccatcagcct gtgcgcgatt ttctattacc ttgccaaata tccgcagtgg
     2461 caggagcggg tgcgggcaga atcacgcgcg ctgcaaaaag atacgctgga ccacgacgat
     2521 ctggcgaaac tggaaacgct cgatcgcgtc atcaaggaag ccatgcggct ggtgtcgccg
     2581 gtgcacggta ttccgcgccg gacggtgaag gaagtggaat tccaggggca tgtgattccg
     2641 gcggatacct tcgtcatgat cacgccgatg gtaagtcatc atctgccgga ccactggctg
     2701 gacccggagc agttcgatcc cgaccgattc gcgccgggtc gctcagagca caaggggcat
     2761 ccgtaccagt acctgccgtt tggcggcggc gctcacatgt gcatcggcct gcatttcgcc
     2821 gacctgcaga tcaaatcgat cctgcatcag gtgttgctgc gttatcgctg gagtgtgccg
     2881 gcggattatg tgatgccgct ggatctgacg tcgctgccct cgccttccga tcaattgccg
     2941 gtgacgctgg agcggctgta atcacgtgcg tctttccatt tgcttgcctg aattattttg
     3001 ctgactactt ttgctgatta atatcccggg agtacctaga agccatgagc accctgaaac
     3061 cgcgtcaaac cgttttgatc accggctgtt cctcgggcat tggccgcgcc acggcactgc
     3121 acttccagcg cgccggctgg aacgtggtgg ccaccatgcg cacaccggaa caggaaaccg
     3181 aactcaaccg tctgaacaat gtgctgctgg tggcgctgga tgtgcaggat caggccagca
     3241 tcgataacgc gctgcagcag gcactgaccc atttcggcca gatcgatgtg ctgatcaaca
     3301 acgccggtta tgccttgatg ggcgcgtttg aaaatgccag cgaggagcag atccagcgcc
     3361 agttcgacac caacgtgttc ggcctgatgc gcgttgcgcg cgcggtgttg ccgcattttc
     3421 gcggacgcaa tgccggcctg ctgatcaacg tggccagcat ggcgggccgt ctgccgattc
     3481 cgctgatgag cgtgtacaac gccagcaagt gggcggtgca gggtttttcc gaatgcctga
     3541 cctatgaact ggcgccgttc aatatccgtg tgaagatcgt ggagccgggc gcggtcaaca
     3601 ccgcgttttt cggtcgttcc ggcgatcgcc agaatgctac cggcgtagcg gcctacaatg
     3661 aatttaccaa cgcgcagttt ccggtgatcg acaagaccgg ccccggcggc agcaccagcg
     3721 aggatgtggc cgccaccctt taccgcgccg ccaccgatgg cagtgcgacc ctgcgctatg
     3781 cagtgggcgt cgatgcgaaa gccctgctgt cggcgcgcaa gatgctgccg gaccgcgtgt
     3841 tcctgccgat gattggctgg tcgctgtcgc cggcggtgtt caaccggctg gggcggctgt
     3901 tttacaagcc cacctgaggc accgatccac aggatgtgtc ggcccgggtg accaaaaccc
     3961 ccacaatttt gtcattgttg cccaagtgtc aagccacttg aaagtctcta caatcccctc
     4021 tgtcgtttta cagaataaaa aacagagggg aatttcatga gcgagctttt ttccatccgc
     4081 ggcagaattg cgctggtcac cggtggcacc ggcggcatcg gctacatgat tgcacaggga
     4141 ctggtgaagc acggcgcgaa agtctatgtg gcgtcgcgca aggccgatgc ctgcgagaaa
     4201 accgcgcgcg aactgtcggc gttcggcacc tgcatcggca ttccggccga cgtctccacc
     4261 gaagccggtt gcgccgcgct ggtgaacgaa ttgcaacagc gtgaagacaa gctgaatatt
     4321 ctggtgaaca acgccggcgt cacctggggc gccagcttcg acacctatcc cggtgaagcc
     4381 tgggacaaga tccagaacct gaatgtgaaa tccgctttcg tgatgacgca gaaactgaca
     4441 ccgctgctga gcgctgccgg caccgccgat gatcccgccc gcgtcatcaa catcacctca
     4501 gt
//
LOCUS       DASUEG010000579         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1736368, whole genome shotgun sequence.
ACCESSION   DASUEG010000579 DASUEG010000000
VERSION     DASUEG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736368"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX11_10845"
     CDS             <1..294
                     /locus_tag="VFX11_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4939165.1"
                     /translation="ELGKREGPLPGLDDIADRLHTTPRTLRRKLQDNGTSYRKICQEH
                     QLQLALDSLHDARQKTAAIAHKCGFRDAASFREAFKRWTDLTPQEYRQRLRTR"
     gene            complement(505..1272)
                     /locus_tag="VFX11_10850"
     CDS             complement(505..1272)
                     /locus_tag="VFX11_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939166.1"
                     /translation="MTNTTEFQSELSAIQSIPLDAVKKPIMPIRQYIFEAEFLHRWSQ
                     ADRPLLEKAGLDLSLLDAILNRAGALREAESLWSLERFSREDAQKEWEEQSPAAYALR
                     DKLLRDMRFAFRRDDALLARVADIADGAGHVDMLQDLNDLALLGRTQLVALAGLGVDE
                     ALLDQAAALADQLTTLLASAAGERKEGNSTRQLRDQAYTHLKAAVDEVRDFGQYVFWD
                     NAIRRDGYFSDYTRRANARSQRNRAEANKTGEVTTDA"
     gene            1588..2508
                     /locus_tag="VFX11_10855"
     CDS             1588..2508
                     /locus_tag="VFX11_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX4939167.1"
                     /translation="MTADQNDHENIDDGQLPLFELETPTAEAPSPTEAHESLFQRAVA
                     ELPPSPFSKYIVYVDESGDHSLQSIDNNYPVFVLAFCVFHKRHYSEQVVSALEKFKFN
                     YFGHDQIILHEQEIRKERGAFNIFRSKEQRIQFMDELTGIIERSNFILISCVIDKRAL
                     SKQQGVSANPYHIALGFCLETLHEFLQEKNQHTLKTFVVVECRGKKEDNELELEFRRV
                     CDGNNRLGISLPFEVLFADKKVMSSGLQLADLVARPIGLQVLKPEQENRAFDVLKRKF
                     YCDGGRANVGEGYEGRGSKIYPASESEKPR"
     gene            2636..>2857
                     /locus_tag="VFX11_10860"
     CDS             2636..>2857
                     /locus_tag="VFX11_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939168.1"
                     /translation="MTNAHDPISDLAAEADSGLLEIISRYTQTFLWLQRYDEGMLTEP
                     TGQSGGTLPSETQATAALAQLKASLMARGE"
BASE COUNT          673 a          777 c          843 g          564 t
ORIGIN      
        1 gaactgggca agcgcgaagg gccgctgccc gggctggacg acatcgccga ccgcctgcac
       61 accacaccgc gtactttacg ccgcaaactg caggacaacg gcaccagcta ccgcaagatc
      121 tgccaggaac accagctgca gctggcgctg gacagtttgc atgacgcgcg ccagaagact
      181 gcagctattg cgcataaatg cgggtttcgc gatgcggcca gttttcgtga ggcgttcaag
      241 cgctggacag atctgacacc gcaggagtat cggcagcggt tgcggacgag gtgagaattg
      301 ggggtgaaga tagtggaatc aggaagcggt actcctgcac aggaaacccc gctgccggca
      361 taggaagggg tcagccgtga ataggagggg gttgctgctg cataggaacg ggtgggtgtt
      421 gcataggaca gcggcaagct tgcatagggc gggctgaaga ccgcctggga gccagtgcga
      481 cccccagcgg acggtgggaa cggctcaggc gtcagtcgtc acctcgccgg ttttgttggc
      541 ctctgcccgg ttgcgctggg agcgggcatt ggcccggcgg gtgtaatcgc tgaaatagcc
      601 gtcacggcgg atcgcgttat cccagaacac gtactggcca aagtcacgca cctcgtccac
      661 ggccgccttc aggtgcgtat aggcctgatc ccgcaactga cgggtgctgt tgccctcctt
      721 gcgttcgccg gcggccgagg ccagcagggt ggtcagttga tccgccagcg cggcggcctg
      781 atccagcagc gcctcatcca caccgagacc ggccaacgcc accagttgcg tgcgccccag
      841 aagcgccaga tcgttcagat cctgcagcat gtccacgtgg ccagcgccat cggcaatgtc
      901 cgccacccgt gccagcaagg cgtcgtccct gcggaaggca aagcgcatgt cgcgcagcag
      961 cttgtcacgc agggcgtaag ccgccgggga ctgctcttcc cattcctttt gcgcgtcctc
     1021 gcggctgaac cgttcgaggc tccagagtga ttcggcttcg cgcagggctc cggcccggtt
     1081 gaggattgcg tccagtaaag acagatccag ccccgccttt tccagcagcg gacgatcggc
     1141 ctgactccac cggtgcagga actcggcttc gaagatgtac tgcctgatgg gcatgatggg
     1201 ctttttgacg gcgtcgaggg ggatggactg gatggcagac agttcggatt ggaattcggt
     1261 ggtgttggtc atgggggact ccttgcgcgg tgcgctggtt gaatgtgtgt ttgcaactga
     1321 tttgggcccg gtgcgcttgc gcgctttgat ggacttgttt tgatagacag gagtagagaa
     1381 agaaaatgga gatttctaaa gtattgccga acacaattca gccggataaa gtccggaact
     1441 actattatta ggtttcggtg accgccacgg cagcgcacgc agcacctact atgtgaaggc
     1501 actatgacat cccggatcac cctatatcag gcagacgatg gacagccctg gcgggcagcg
     1561 ccaatcccgc acattcatgg ataaaaaatg acagctgatc aaaacgatca cgaaaatata
     1621 gacgacgggc agttaccact atttgagctg gaaacaccta cggcggaagc accatcccct
     1681 acagaagcgc atgaaagtct gtttcaacgc gctgtggctg aactaccgcc aagcccgttc
     1741 agcaagtaca ttgtttatgt ggacgaaagc ggcgatcaca gcctgcaaag catcgacaat
     1801 aattatccgg tgttcgtgct ggcattctgc gtttttcata aacgccatta cagcgaacag
     1861 gttgtttcag cactcgaaaa attcaagttt aattactttg gacatgatca gatcattctg
     1921 cacgaacagg agattcgcaa ggaaagaggc gccttcaata tttttcgcag caaagagcag
     1981 cgaatccagt ttatggatga gttaaccggc attatcgagc ggagtaactt catccttatc
     2041 agttgcgtga tagataagcg tgcattgagc aagcaacaag gtgtgagtgc caatccctat
     2101 cacatcgctt tgggcttctg cctggaaacg ctgcatgagt tcctgcaaga gaaaaatcaa
     2161 cacacgctca aaacctttgt agtggttgaa tgcagaggca agaaggaaga taacgaactt
     2221 gagctggagt ttcgccgtgt ttgtgacggc aacaaccgtc tgggcatctc actgccattt
     2281 gaagtgctct ttgctgacaa gaaagtcatg tcttcaggac tgcagttggc cgatctggtg
     2341 gctcgtccaa tcggtctgca ggtgttgaag cctgaacagg aaaaccgggc ctttgatgta
     2401 ttaaagcgga aattctattg cgatggggga cgcgcaaacg tcggtgaagg ttatgaaggt
     2461 cgaggatcta aaatttaccc cgcctcagaa agcgaaaagc cccggtgaaa cccaccgagg
     2521 ctatgacgcc gaccgggcac ccccgatcca cttgtgcgaa gtatagataa taaaccgacg
     2581 gatgggcaac acggaaatca ccgcaattca tagaaaacca cccaggagcc caaccttgac
     2641 caacgcccac gacccaatat ccgaccttgc cgccgaggca gactccgggt tgctggaaat
     2701 tatcagccgc tacacccaga ctttcctgtg gctgcaacgc tatgacgagg gcatgctgac
     2761 cgagcccacc gggcaaagcg gcggcaccct gccgagcgag acgcaggcca ctgccgcgct
     2821 ggcacagttg aaagcatcgc tgatggcgcg gggcgag
//
LOCUS       DASUEG010000580         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_8069, whole genome shotgun sequence.
ACCESSION   DASUEG010000580 DASUEG010000000
VERSION     DASUEG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8069"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFX11_10865"
     CDS             <1..736
                     /locus_tag="VFX11_10865"
                     /inference="COORDINATES: protein motif:HMM:NF039651.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative solute-binding protein"
                     /protein_id="HEX4939169.1"
                     /translation="SLDAVGALPSMKHMQTFIQMFSTNPKMNEFTIAGDYQTIAVIPL
                     GAAYVIVNDRKIDSIEKAAGKKVAVLEWDKSQARMVQQLGAQPVASDITNFAGKFNNG
                     QVDIIAAPALAFKPLELYRGLGTKGAIYRMPLVQVTGSVMIRRAKFVKEVPDFDKLVL
                     KLRQYAVTKLPTVYNMIDKSEKEIDARYWLDLPTAEKQKYERMMREARMQLTREGVYD
                     PKMMAILKKVRCKLDPANFECALKDE"
     gene            complement(776..1342)
                     /locus_tag="VFX11_10870"
     CDS             complement(776..1342)
                     /locus_tag="VFX11_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX4939170.1"
                     /translation="MKNMTPHNNLPLAQGLVKLEELEYRLCMMFNRTSRTIAVRRFFR
                     LVSRLGDGMFWYALMAALAMFQGTAGRIAALQMGLTALIGVIIYKLLKENLVRERPFI
                     TYQSIECGCPPLDRYSFPSGHTLQAVLFSTLALAWFPGLAVLLVPFTVLVALSRMVLG
                     LHYPTDVICGAMLGGALAGCSLWLFMPA"
     gene            1492..2544
                     /locus_tag="VFX11_10875"
     CDS             1492..2544
                     /locus_tag="VFX11_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4939171.1"
                     /translation="MKIAICTDAWSPQVNGVVTTLHRTRDCLKQFGHDVEVFSPASGF
                     LTFPLPSYPEIRLPLFPGRRLARMLDEFSPDCLHIATEGPLGLAARAWCVQRGLPFTT
                     SYHTQFPEYVHARLPIPVALVYRLLRWFHGGAAATMVSTTHMREQLRHHGFSNLVAWG
                     RGVDTQLFSPQRRGTLDLPRPVWVYAGRLAVEKNLPAFLALDLPGSKLVIGDGPARAV
                     LQQQFPDAHFVGYQFGEALARYMASGDVFVFPSCSETFGLVMLEAMACGLPVAAFPVT
                     GPIDVVRQGISGVLHEDLQTACLAALSLDRQQVRQTALEYSWEQASRQFESHLVPMRK
                     PALLPPRRLAPPAQAT"
     gene            2800..>3653
                     /locus_tag="VFX11_10880"
     CDS             2800..>3653
                     /locus_tag="VFX11_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4939172.1"
                     /translation="MLVCLSGLRPAVAAVSPLVLSPGESRFPSYAALRHFCTPSDQSP
                     SARALLSRLNEWPWQPSGPDVPNHGFSQEVCWFHLVVKNEGHTNNHWFLVANYPLIEE
                     LDVHVVAPDGHITASHATGSNRPFWQRALPVQAYAFPFELRDGQTLSFLLRVHTPSLQ
                     LPLEVIEPDHFAGMVVKSTLLQGVFLGGMVVMILYNLFLYASIREPAYLLYVLYSVVI
                     TLIQADNHGLLHRFLWPQAGVYALPWLMPLMVVLASLFTIHFLALTRRAPFWARLLQA
                     HVAIAVVL"
BASE COUNT          651 a         1072 c         1146 g          784 t
ORIGIN      
        1 cagtctggat gccgtgggtg ccttgccctc gatgaagcac atgcagacct tcatccagat
       61 gttttccacc aatcccaaga tgaatgagtt cactatcgca ggcgattacc agaccatcgc
      121 cgtgatcccg ctgggtgcgg cgtatgtgat tgtgaacgat cgcaagatcg actcgattga
      181 aaaggcggcc ggcaagaaag tcgcggtgct ggagtgggac aaatcgcagg cgcgcatggt
      241 gcagcagctg ggcgcgcaac cggtggcttc cgacatcacc aattttgccg gcaagttcaa
      301 caacggtcag gtcgacatca ttgcggcccc ggcgctggcc ttcaaaccgc ttgagctgta
      361 tcgcggtctg ggcaccaagg gtgcgattta tcgcatgccg ctggtgcagg tcaccggctc
      421 ggtgatgatt cgccgcgcca agttcgtcaa ggaagtgccc gacttcgaca agctggtcct
      481 gaagttgcgt cagtacgccg ttaccaagct gccgacggtg tacaacatga tcgacaagag
      541 cgagaaggag atcgacgcgc gttactggct ggatctgccg acggcggaga agcagaagta
      601 tgaacgcatg atgcgcgagg cccgcatgca gctcactcgc gagggtgtgt acgatccgaa
      661 gatgatggcg attctgaaga aggtgcgctg caagctcgat ccggcgaatt tcgagtgcgc
      721 gctcaaggac gagtaagtgg tggagatgac caccggcctg atgacgggcc ggtgatcacg
      781 ccggcatgaa cagccagagc gagcatccgg ccagcgcgcc accgagcatg gcgccgcaga
      841 tgacatcggt tggatagtgc agtcccagaa ccatgcgtga cagggcgacc agtacggtga
      901 agggaaccag cagcaccgca aggcccggaa accacgccag tgcgagcgtg ctgaacagga
      961 cggcttgcag ggtgtgtccg gacggaaagc tgtagcggtc cagcggcgga caaccacact
     1021 cgatgctctg gtaggtgatg aagggccgct cacgcaccag attttctttc agcagtttgt
     1081 agatgatcac gccaatcagc gcggtcaggc ccatctgcag ggcggcgatg cgtcccgcag
     1141 tgccctgaaa catcgccagc gcagccatca gcgcatacca gaacatacca tcgcccaggc
     1201 ggctgacgag gcggaaaaat cgtcgtaccg cgatggtgcg cgacgtgcgg ttgaacatca
     1261 tgcaaagccg gtattccagt tcttccagct tcaccagtcc ctgcgccagc ggcaggttgt
     1321 tgtgtggcgt catgtttttc atgccttcat gctggaagcg tgctgcaaca gtcctgtgtc
     1381 atctttctga cggtttggtg acagccgcct gatggcgaca tgagttcacg gatcacagca
     1441 tcgtcatcgc acggacacca tcccgtcatc tggtcgcggc aaggtggcgg catgaaaata
     1501 gccatctgta ccgacgcctg gagtccgcag gtcaacggcg tggtgaccac gctgcaccgc
     1561 acccgcgatt gcctgaaaca gttcggccat gacgtggagg tgttttctcc cgccagtggc
     1621 ttcctcacgt ttcccctgcc cagttatccc gaaatacggc tgcccctgtt tccgggacga
     1681 cgcctggcac ggatgcttga cgagttctcg cctgactgcc tgcacattgc cacggagggg
     1741 ccgctgggtc tcgcggcgcg agcctggtgt gtgcagcgcg ggctgccctt taccacgtcc
     1801 taccacacac aatttcccga gtatgtgcat gcccgtttgc cgatcccggt agcgctggtg
     1861 taccgcttgc tgcgttggtt tcacggcgga gcggcggcca ccatggtgtc cacgacacac
     1921 atgcgcgagc agttgcgtca ccacggattc tcaaatctgg tggcgtgggg gcgcggcgtg
     1981 gatacgcaac tgttttcccc gcagcgccgc ggcacgctgg atctgcctag gccggtgtgg
     2041 gtgtatgccg ggcgtcttgc cgtcgagaag aacctgccgg cctttctggc gctggatctt
     2101 cccggcagca agctggtgat tggcgatggt ccggcccgtg ctgtcttgca gcagcagttt
     2161 cccgatgcgc attttgtggg ttatcagttc ggcgaggcgc tggcacgcta catggccagc
     2221 ggtgatgtct tcgtgtttcc cagttgcagc gaaaccttcg gtctggtgat gctggaagcc
     2281 atggcctgtg gattgccggt cgcggccttt ccggtaacgg ggccgattga tgtggtacgt
     2341 cagggtatca gtggcgtgct gcacgaggat ttgcaaaccg cctgcctggc agcgttgtcg
     2401 cttgaccgtc aacaggttcg gcagaccgca ctcgaataca gctgggagca agcctcccgg
     2461 cagtttgaaa gccatcttgt gcccatgcgc aagccggcct tgcttccgcc acgtcgattg
     2521 gcgccgccgg cccaggcaac ctgagtcagg ctccctcctt gtgcatgccc tgctcctgcc
     2581 agagcagggc atgtgcgcat ctgtgcgctg ggcagcatat actgcatgaa cggcgggttg
     2641 ttcctcggta gttgcccacc aatcctttgc cagaggggat gatgtgcagg cgtgatggtg
     2701 ttcacacgcg cctgtttccc cgcatacgga caaacagggc gggccagcaa catgccaggg
     2761 aggctttggc gtttatcgtg gttgagcggt gtgtggctct tgctggtctg cctgtcaggg
     2821 ttgcgaccgg cggtggccgc tgtctcgccg cttgtcctgt ctcccggtga aagccgtttt
     2881 ccgtcgtacg ccgcgctgcg tcatttctgc acgccatccg atcagtctcc ctctgctcgt
     2941 gccctcctgt cgcgcctgaa cgagtggcca tggcagccct ccgggcctga tgtccccaat
     3001 catggcttta gtcaggaggt ctgctggttt caccttgtcg tgaagaacga ggggcacacg
     3061 aacaatcact ggttcctggt ggcgaattac ccgctgatcg aggagctgga tgtgcatgtg
     3121 gtagcgcctg acgggcacat cacggcgagc catgccacag gcagtaatcg tcccttctgg
     3181 cagcgcgcgc tgccggtgca ggcctatgcc tttccgtttg agttgcggga cggacagaca
     3241 ctgtccttcc tgctgcgtgt gcatactccc agcctgcagt tgccgctgga ggtgatcgag
     3301 cctgaccatt ttgccggcat ggtggtgaag agcacgttgc tgcaaggcgt gtttctcggc
     3361 ggcatggtgg tgatgattct ctacaacctg ttcctctatg cctcgatccg ggagccggcg
     3421 tatctgctgt atgtgctgta ctccgtcgtg atcacgctga tacaggccga caatcacggc
     3481 ttgctgcacc gctttctgtg gccgcaggcc ggcgtgtatg cgttgccatg gctgatgccg
     3541 ctcatggtgg tgctggcctc cctgttcacc atccactttc tggccctgac aagacgcgca
     3601 ccgttctggg cgaggctgtt gcaggcgcat gtggcaattg ccgtcgtgtt gat
//
LOCUS       DASUEG010000581         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2010757, whole genome shotgun sequence.
ACCESSION   DASUEG010000581 DASUEG010000000
VERSION     DASUEG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010757"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFX11_10885"
     CDS             <1..834
                     /locus_tag="VFX11_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939173.1"
                     /translation="DDERLMDKSLAFAKNRAGELVIRTDTAHWQGFPIWCGDGCKYVP
                     IPFTRTVSHAWSRWPSVPIPVYQLSSSTVAPEAALDADAQTHAPADETPAGRVTRLLR
                     GIAPLGVRVTGVSPDGERWIGRFQGNADDLIAMHEALMSSGAVADLMVHPVEGRKGSE
                     RMIEFSFVATALQGGRKAAETTAAVEQKQAKAVAAQEEEDNRVAKLFLGTLPTGATLT
                     SWVREGDGYQARVQCPDDGMVYTVIVNANVSGHFSRVGLKSKVREQSGMTSAEITLGL
                     D"
     gene            complement(878..3109)
                     /locus_tag="VFX11_10890"
     CDS             complement(878..3109)
                     /locus_tag="VFX11_10890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007106988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4939174.1"
                     /translation="MQHTDLLIDGAKCASCVSKIETALQKVPGVQSASMNLAQRSASI
                     DGSAAPEDLVCAIEQAGYKATVVQSENEADLLSESEQADAKNYRTRLRDMVLALALGA
                     PLMIYGVLGGEMMIHTPQQQIGWLVIGVLTLAVLVFPGRHFFVGAWKSIRNRTATMDT
                     LIALGTGTAWVYSMLVAAFPHFFPEQARHVYFEASAMIIGLINLGLALEIKARGRTSQ
                     AIKRLIGLQVKTARVIRNGNEVDIPLALVQVNDQVRVRPGEKIPVDGVVSEGHSSIDE
                     SMLTGEPMPVEKSVGTEVAAGTLNKTGTLVFTATRVGKDTALARIIDMVKRAQSSKPA
                     IGRLADAIASVFVPAVMLIAVASAVAWFIFGPQPALSFALVSATTVLIIACPCALGLA
                     TPISIMVGVGKAAENGILIRNGDALQTAAKLDIMVLDKTGTITQGTPRVTAIHVTGNA
                     DENVVLQLAASLEAGSEHALAQALTDAAQEKKLPLLPAENFTAIPGQGVRGNVNRKNL
                     LLGNRKLMQENSISLHTHEARAQQLAAQGQTPVFFAVDNALAAIIAIADPVKSDSVAA
                     IQRLQRHGMRVIMLTGDNAVTAKAIADQVGITEFFAEVLPQHKADKVKELQADGAIVG
                     MTGDGINDAPALAQADVGFAIGTGTDVAIESADITLMRGSLHGIADAIAVSQATLRNI
                     RQNLLGAFIYNVAGIPIAAGVLYPFTGLLLNPVLAGAAMALSSVTVVSNANRLRWFKP
                     AQH"
BASE COUNT          576 a          905 c          979 g          655 t
ORIGIN      
        1 gatgacgagc ggctgatgga caagtcgctg gcgtttgcga aaaatcgcgc cggcgaactg
       61 gtgatccgta ccgataccgc gcactggcag ggttttccca tctggtgcgg cgacggttgc
      121 aagtatgtgc ccattccttt cacgcgcacg gtttcccatg catggtcgcg ctggccgtcg
      181 gttccgattc ccgtctatca gctctcgtct tcgacggttg cacctgaagc ggcgctggat
      241 gccgatgcac aaacccatgc gcctgctgat gaaacgccgg ccggtcgtgt tacgcgtttg
      301 ctgcgcggaa tcgcaccgct gggtgtgcgc gtgaccggtg tgagccccga tggcgaacgc
      361 tggattggcc gtttccaggg caatgccgac gacttgatcg ccatgcacga ggcgctgatg
      421 agttcggggg cggtggctga tctcatggtg catccggtgg aaggtcgcaa aggctcggaa
      481 agaatgatcg agttcagttt cgtcgccacg gcgttgcagg gtggacgcaa agctgccgag
      541 accacggcag cagtggagca aaagcaggcg aaggctgttg cggcgcagga agaggaggac
      601 aatcgtgttg caaaactgtt tctcggcacg ttgcccacag gtgcgacact gacatcctgg
      661 gtaagggaag gtgacggcta tcaggccaga gtgcagtgcc cggatgacgg catggtttat
      721 accgtcattg tgaatgccaa tgtctcaggg catttctcgc gggttggtct caaaagcaag
      781 gtacgcgagc agagcggtat gacgagcgcc gagattacgc tggggctgga ctgatctggt
      841 caatggcgga gggcaaatcc ctttgcccgg gctgccttca atgctgcgcc ggtttgaacc
      901 agcgcagtcg gttggcgttg ctcaccacgg tgaccgatga cagcgccatc gccgcacccg
      961 ccagcaccgg attcagtaac aggccggtga aggggtagag cacgcccgcc gcgatgggaa
     1021 ttccggccac attgtagata aacgcgccca gcaggttctg gcggatattg cgcagggtgg
     1081 cctgactgac ggcaatcgca tccgcaattc cgtgcagtga tccccgcatc agcgtgatgt
     1141 cggcactttc gatcgccacg tcggtaccgg tgccaatggc aaatcccaca tccgcctggg
     1201 ccagcgcagg ggcgtcgttg atgccgtcgc cggtcatgcc gacgatggcg ccatcggcct
     1261 gcaattcctt cactttgtca gccttgtgtt gcggcagaac ctcggcaaaa aattcagtaa
     1321 taccgacctg atccgcgatg gcttttgcgg tgacggcgtt gtcgcccgtg agcatgatga
     1381 cccgcatgcc atgccgttgc agacgctgga tggcagcgac ggaatcgctt ttcaccggat
     1441 cggcgatggc gatgatggcg gccagcgcat tgtccaccgc gaagaagacc ggcgtctgtc
     1501 cctgcgcggc cagttgctgc gcgcgggctt cgtgcgtatg caggctgata ctgttttcct
     1561 gcatcagttt gcgattgccc aataacagat tcttccggtt aacgtttcca cgtacgccct
     1621 ggccgggaat ggcggtgaaa ttctctgctg gcaacagcgg cagtttcttt tcctgtgcgg
     1681 cgtcggtgag tgcctgtgcc agtgcgtgct ccgacccggc ttccaggctg gcggccagtt
     1741 gcagcacaac attttcatct gcattaccgg tgacatgaat tgcagtaacc cttggtgttc
     1801 cctgtgtaat cgtgccggtt ttgtccagca ccatgatgtc cagcttggcc gctgtctgca
     1861 gcgcatcgcc attgcggatc aggatgccat tttccgccgc cttgccaacg ccgaccatga
     1921 tcgaaatggg ggtggcgagg ccgagtgcac aggggcaggc gatgatcagc acggtggtgg
     1981 cggataccag cgcaaacgac aacgctggct gtggaccaaa gatgaaccag gccacggcgc
     2041 tggcgacggc gatcagcatc actgcaggca cgaacacact ggcaatggca tcggccagcc
     2101 ggccaatggc gggcttggaa ctttgcgcgc gtttcaccat gtcgatgatg cgggccagcg
     2161 cggtgtcctt gccgacgcgg gtggcggtga aaaccagtgt gccggttttg ttgagggtgc
     2221 cagccgccac ctcggtgcca acggattttt ccactggcat gggctcgccg gtcaacatgg
     2281 attcgtcgat gctgctgtgg ccttcgctca ccacaccgtc caccggaatt ttttcgccgg
     2341 ggcgcacccg cacctgatcg ttcacctgta ccagtgccag gggaatatcg acttcattgc
     2401 cgttgcgaat cacccgagcg gtttttacct gcagtccgat caatcgcttg atggcctgcg
     2461 acgtgcggcc acgggccttg atttccagcg ccagccccag gttgatcagg ccgatgatca
     2521 tggcgctggc ttcaaaatag acatggcggg cttgttcggg aaagaagtgc ggaaaggccg
     2581 ccaccagcat ggaatatacc caggcggtgc cagtacccag tgcgatcagc gtgtccatgg
     2641 tggcggtgcg gttgcggatc gatttccatg cgcccacaaa aaaatggcgg ccgggaaaca
     2701 ccagcacggc cagcgtcagt acaccgatta ccagccagcc gatctgctgc tgcggcgtgt
     2761 gaatcatcat ctcgccgccc agcacgccat aaatcatcag cggtgcgccc agtgccagcg
     2821 caaggaccat gtcgcgcagg cgggtgcggt aattcttcgc gtcggcctgt tcgctttccg
     2881 acagcaaatc cgcctcgttt tcactctgca ccacggtggc cttgtagccg gcctgttcga
     2941 tggcgcagac gaggtcttcc ggcgcggcac tgccatcgat actggcgctg cgctgtgcca
     3001 ggttcattga tgcgctttgc acgcccggca ctttttgcag cgcggtttca atcttgctga
     3061 cgcagctggc gcatttggcg ccgtcgatca gtaaatcggt gtgctggatt gcagt
//
LOCUS       DASUEG010000582         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_809731, whole genome shotgun sequence.
ACCESSION   DASUEG010000582 DASUEG010000000
VERSION     DASUEG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809731"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFX11_10895"
     CDS             <1..292
                     /locus_tag="VFX11_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939175.1"
                     /translation="GNMTAGRKIRMVFTTLLAFSLFALESEVMSAPLTPAQRAAKEQE
                     RAHQRQQKQCARYQARLDRINRKLDAGYREPRGNELRQQRRELQSVLFRECR"
     gene            complement(314..1519)
                     /locus_tag="VFX11_10900"
     CDS             complement(314..1519)
                     /locus_tag="VFX11_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX4939176.1"
                     /translation="MHPLLLTAILAGLVAVGPFAIDTYLPALPSMARDLGAPIAWIQA
                     SIPAYLIGGAIGQFFGGPLSDQIGRKPVGLFGLLLYALSSLLIWLCESVNALLALRFT
                     QALGGGCATVIVAATVRDSLDGKNAARMMALIGLIMTTAPLVAPAVGSGLLHLAGWRA
                     IFMMLALYALLMFVLLGWQVPESRARAAQISLRILAAGMVRNYGVVFRHRRAVGYLLG
                     MGFASATMFVFLSSSAFAYMEYFGASESLFPVLFGANILTMMIANRAGVFALRWAEPE
                     LLCRIGVVVLFVMMLLLALYVELFTPTLYGVVPLIMLGVGMMGLIYPQGMASYLHFFP
                     QQAGAASALLGILQFTLGALSGALVNVLHDGSLKPTAFGMAICAFISLAGLLGIAGLS
                     GKDNSAPSH"
     gene            1772..>2149
                     /locus_tag="VFX11_10905"
     CDS             1772..>2149
                     /locus_tag="VFX11_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939177.1"
                     /translation="MSLSHSMRRFGTLPAVAAIGLCLSAGTATAGNLGIAGKISSLGY
                     GLEADYVLSDRFSVRGQFNQYDYDDTFEEDGIDYDGTLDLSTFGVLLDWHPFGGGFRV
                     SAGGFNVDNELSGVGSGAGTYHIG"
BASE COUNT          484 a          699 c          615 g          351 t
ORIGIN      
        1 aggcaacatg acagcaggaa ggaaaatcag aatggtattc accacgctgt tggcgttctc
       61 cctctttgcg cttgagagcg aagtcatgtc ggcgccgctc acccccgccc agcgcgccgc
      121 caaggaacag gaacgcgccc atcagcgcca gcagaaacaa tgtgcgcgct atcaggcccg
      181 gctcgaccgc atcaaccgca aactggatgc cggttaccgc gagccccgcg gcaatgaatt
      241 gcgtcagcag cgacgggagc tgcagtccgt cctgttccgg gaatgtcgct gacgccaata
      301 tcgcgcttcc cgctcagtgc gatggcgcgc tgttatcttt gccgctcaag ccggcaatgc
      361 cgagcaatcc cgccagactg atgaacgcgc agatcgccat gccaaacgcc gtcggcttca
      421 ggctgccatc atgcagcaca ttcaccagcg cgccggacag tgcccccagc gtgaattgca
      481 gaattcccag cagcgcgctg gcggcgccag cctgctgcgg aaaaaagtgc agatagctcg
      541 ccatgccctg cggataaatc agccccatca tccctacacc cagcatgatc agtggcacca
      601 cgccatacag cgttggtgta aacagctcca cgtacagcgc cagcaacagc atcattacaa
      661 acagcaccac caccccgatg cgacacagca gttccggctc cgcccagcgc agcgcaaaca
      721 cgccggcgcg attggcaatc atcatggtga gaatgttggc gccgaacagc acgggaaaca
      781 gcgattcaga cgcgccaaaa tattccatgt aggcaaacgc cgatgacgac agaaacacaa
      841 acatggtggc cgacgcaaag cccattccca gcagataacc gacggcgcga cgatgccgga
      901 acaccacgcc gtaattgcgc accatgcccg ccgccagtat acgcagtgaa atctgcgcgg
      961 cccgggcccg gctttccggc acctgccagc ccagcagcac gaacatcagc agcgcataca
     1021 gggccaacat catgaagatg gcacgccagc cggccaggtg cagcagaccc gatcccaccg
     1081 ctggcgccac cagcggcgct gtcgtcatga tcaggccaat aagcgccatc atgcgggccg
     1141 cattcttgcc atccagtgaa tcccgcaccg tggcagcgac gatcaccgtg gcgcaaccgc
     1201 cgcccagcgc ctgggtaaag cgcagggcga gcagcgcgtt cacggactca catagccaga
     1261 tcagaaggct cgacagcgca tacagcagca ggccaaacag gcccaccggc ttgcgcccga
     1321 tctggtccga cagcgggcca ccgaaaaact ggccaatggc cccgccgatc agataagcgg
     1381 gaatcgacgc ctgaatccag gcaatcgggg cgcccagatc ccgcgccatg gacggcagcg
     1441 cgggcagata ggtgtcgatg gcaaacggac cgacggcgac aaggcccgcc agaatggcgg
     1501 tgagcagaag tgggtgcatc agattccgtc aggcaataaa cgtgagcgtg ttttataccg
     1561 gattgatgag tgattggaca ggtttggtgg ccgtctgaac aacattggcg cgtttacgct
     1621 gttcaaaaca ccgtttcctt gcgccggttc acagcgacga ctcaacgatt tggcgacgat
     1681 tcggaaatcc agcggccaat ggcggcaccc gcaccgggcg gctatgcttg ctgaaccccc
     1741 ctgtcatacc gtcagtatta aggagttcct catgagcctg tcgcattcaa tgcgtcgttt
     1801 tggcacgctg cctgccgtgg cggcaatcgg cctgtgtctg tccgccggta ccgccaccgc
     1861 cggcaatctg ggcatcgccg gcaaaatcag ttccctcggt tatggcctgg aagcggacta
     1921 cgtgctgtcc gaccgtttca gcgtccgcgg ccagttcaac cagtacgact atgacgacac
     1981 ctttgaagag gacggcatcg actacgacgg tacgctggac ctgtccacct tcggcgtgct
     2041 gctggactgg cacccgttcg gcggtggctt ccgtgtcagc gccggtggtt ttaatgtgga
     2101 caatgaattg agcggtgtcg gcagtggcgc cggcacgtat cacattggc
//
LOCUS       DASUEG010000583         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1526149, whole genome shotgun sequence.
ACCESSION   DASUEG010000583 DASUEG010000000
VERSION     DASUEG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526149"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..727)
                     /locus_tag="VFX11_10910"
     CDS             complement(35..727)
                     /locus_tag="VFX11_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX4939178.1"
                     /translation="MSLWMKLHTLFRATATEPLEHIVDVNAIRIFEQEIRDAENAIVQ
                     AKYQLATVMAEKKQLQRHNQALEENIANKERQTLAALEKNEEALAQDIAALIADDESL
                     LADQRKQADYLQQQETHLKRQLRIAAQSIQKYQRELALAKANNSAQKALGQLKGYSSG
                     LNASMQDMASSLERIQKRQNRAVDMDAALSEINAEMSGEQLDARLKQAGIKTGKHDAE
                     AVLERLRKQRAA"
     gene            complement(733..1002)
                     /locus_tag="VFX11_10915"
     CDS             complement(733..1002)
                     /locus_tag="VFX11_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HEX4939179.1"
                     /translation="MLDNIQSNSNGWLFFVKTSFAIALLATTAGILFTPIDLLVKGYL
                     GICALFLVSTTITLSKTMRDEHESQRLIHKISEAKTKQLIKEFAE"
     gene            1151..1900
                     /locus_tag="VFX11_10920"
     CDS             1151..1900
                     /locus_tag="VFX11_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4939180.1"
                     /translation="MAQTTALIDALKRVLREQGITYAEVAQRLDLSLPSVKRLFADKQ
                     FSVQRLDQVCELAGIEITDLVRRMQADKEIHQLEWEQEQELVSDPKLLIVAVSALNRW
                     SFRDILATYKIKEPELIQRLARLDRMKLIELMPGNRIRPLASTDFQWHRGGPIQRFFE
                     AQVQNDFFRCTFEKPGELRLFVTGMLSRESNGQLQQKMQRLAQDFRNLHQEDQALPLA
                     DRFGTSLVLAMRPWELEIFRQHRRADTDKPF"
     gene            2059..3534
                     /locus_tag="VFX11_10925"
     CDS             2059..3534
                     /locus_tag="VFX11_10925"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4939181.1"
                     /translation="MSNDWIYGSARQLLQSLQKGEISSRELLETYIARIERFNRDINA
                     VVANDFANARQRADEADAARSKGESWGPLHGLPITVKDTYEVPGMPCTAGASSLRNHM
                     PKKPAVAIKHLLDAGAIIVGKTNVPVFASDLQSYNKVYGTTHNPWNKALTPGGSSGGS
                     AASLAAGFSALELGSDIGGSIRIPAHFCGVYGHKVTHGIVSLRGHIPGPPGGLGEPDL
                     AVAGPMARTAEDLQLLLDVVAGPSPLMQPGWQLKLPAAKQKKIEDFRVLLWIDDPLCP
                     IDQRMTAVYGQMKIALENAGVKVTLGSPLGLGLADFYPSYMNLMGSIMGASRKKVERR
                     LMGLAAPLLEKIGQHFDTPKGFEHFFTGAAQSHVDWLRVDEKRRRIREKFLKVFDEYD
                     VILMPPAATTALPHQHQPEMPLRKITINGEKRHYADMLIWISPATLMGLPATSAPVGV
                     TSDNLPVNVQVVGAPFQDKTTIKFAALMAKVVGGFVVPPGY"
BASE COUNT          686 a          989 c         1186 g          807 t
ORIGIN      
        1 cccggcgaca acaaggctgt ttgccgccgg tccgtcaggc cgctcgctgc tttctgagcc
       61 gctccaacac tgcctcggca tcgtgcttgc cggttttgat gcccgcctgt ttcagccgtg
      121 catccagttg ttcccctgac atctcggcat tgatctcact cagcgccgcg tccatgtcca
      181 cggcacggtt ctgccgtttc tgaatccgtt ccagtgagga cgccatgtcc tgcatggacg
      241 cattcagacc actcgaatag cccttcagtt gtcccagtgc tttttgcgca ctgttgttgg
      301 ctttcgccag cgccagttcg cgctggtatt tctggatcga ctgcgcggcg atgcgcaatt
      361 gccgtttcag atgggtttcc tgttgctgca gatagtccgc ctgtttgcgc tggtctgcca
      421 gcagggattc atcatcggcg atcagagcgg cgatgtcctg tgccagcgct tcttcatttt
      481 tctccagcgc cgccagtgtc tggcgttcct tgttggcgat gttttcctcc agcgcctggt
      541 tgtggcgctg cagttgcttt ttctccgcca tcaccgtggc cagctggtat ttggcctgca
      601 cgatggcgtt ttcggcgtcg cgaatttcct gttcgaagat gcgaatcgcg ttgacatcga
      661 cgatgtgttc cagcggttcg gtggcggtgg cgcgaaacag ggtgtgcaat ttcatccaca
      721 gactcatggc aattactccg caaattcctt gatcagttgt ttggttttgg cttcggaaat
      781 cttgtgaatc aggcgctggc tttcgtgttc gtcacgcatg gttttcgaca gcgtgatggt
      841 ggtgctcacc aggaacagcg cgcagatgcc cagatagcct ttcaccagca ggtcgatcgg
      901 ggtgaacagg atgcctgccg tggtggccag cagggcgatg gcgaatgagg ttttcacaaa
      961 gaacagccag ccgttcgagt tactttggat gttgtccagc atggggtttg ctccggtcag
     1021 agggttgttg aaagcagcgg gatcagtatc gggcagggtg ttggatatgg ctaccaaagc
     1081 cttgaatgtc gcgatatgtg gtgctatagg tatcgtatat tgataccttt tcgtgcgaag
     1141 tgaggtgccc atggcccaga ccactgccct gatcgatgcc ctgaaacgcg tattgcggga
     1201 gcaggggatt acctatgccg aggtggcgca acggctggat ttgtcgctgc ccagcgtgaa
     1261 gcggctgttt gccgacaagc agttttcagt gcagaggctg gaccaggtgt gcgaactggc
     1321 gggcatcgag atcacggatc tggtgcggcg gatgcaggcg gacaaggaaa tccaccagct
     1381 ggaatgggag caggaacagg aactggtgtc cgatcccaag ctgctgattg tggcggtgtc
     1441 ggcgctgaac cgctggagtt ttcgcgacat tctggccact tacaaaatca aggagccgga
     1501 gctgatccag cgtctggcgc ggctggatcg catgaaactg atcgaactga tgcccggcaa
     1561 tcgcatccgg ccactggcgt cgacggattt ccagtggcac aggggcggcc cgatccagcg
     1621 cttttttgaa gcccaggtgc agaacgattt tttccgctgc acctttgaga agccggggga
     1681 attgcggctg ttcgtgaccg gcatgctgtc gcgggaatcc aatggtcagc tgcagcagaa
     1741 aatgcagcgg ctggcgcagg attttcgcaa cctgcatcag gaggatcagg cgctgccgct
     1801 ggcggaccgc ttcgggacca gcctggtgct ggccatgcgg ccctgggaac tggaaatatt
     1861 ccgccagcat cgccgggccg acactgacaa gcctttctga tgaaggtcat gcattgtgaa
     1921 cattggtcag ggcgaccgga aatatgacgc gttctgtcat ttgcaagcgt gtgcaatgcc
     1981 gctacccttt cggcaggttt tttggtcctg ttcaggcaag tgtcctggca atcgttctga
     2041 aatgcagagg ggaaccccgt gagtaatgac tggatttatg gcagtgcccg gcaattgctg
     2101 cagagcctgc agaaaggcga gatcagcagc cgcgaactgc tggaaaccta tattgcccgc
     2161 attgaacgct tcaaccggga catcaacgcg gtggtggcga atgattttgc caacgcgcgc
     2221 cagcgggcgg atgaagcgga cgcggcgcgg tcgaaagggg aatcctgggg cccgctgcat
     2281 ggtctgccga tcacggtgaa ggacacctat gaagtgccgg gcatgccctg cacggcgggt
     2341 gcgtcgtcgt tgcgcaacca tatgccgaag aagccggcgg tggcgatcaa acatctgctg
     2401 gatgccggcg ccatcatcgt cggcaaaacc aacgtgccgg tgttcgcgtc cgacctgcaa
     2461 agttacaaca aggtgtacgg caccacccac aatccctgga acaaggcgct gacgcccggc
     2521 ggttcgtccg gcggttcagc ggcgtcgctg gcagcgggat tttccgcgct ggagctgggc
     2581 agtgacatcg gcggttccat ccggattccg gcgcatttct gcggtgtgta cgggcacaag
     2641 gtgacgcatg gcattgtgtc cctgcggggc catattccgg gcccgccggg tgggctgggc
     2701 gaaccggatc tggcggtggc cggccccatg gcgcgcacgg cggaggatct gcaattgctg
     2761 ctggatgtgg tggcgggtcc gtcgccgctg atgcagccgg gctggcagct gaaactgccg
     2821 gcggcgaagc agaaaaagat cgaggatttc cgcgtgctgc tgtggatcga tgatccacta
     2881 tgccccatcg atcagcgcat gacagcggtg tacggccaga tgaaaatcgc gctggaaaat
     2941 gccggtgtga aggtgacgct gggttcgccg ctgggattgg gccttgcaga tttctacccg
     3001 tcgtacatga acctgatggg cagcatcatg ggagcgtcgc gcaagaaggt ggagcgtcgc
     3061 ctgatggggc tggcggcgcc gctgctggaa aagatcggcc agcatttcga tacgcccaag
     3121 ggcttcgagc atttcttcac gggcgcggcg cagagccatg tcgactggct gcgggtcgat
     3181 gaaaagcgcc gccgcattcg cgagaaattc ctgaaagtat tcgatgagta tgatgtgatc
     3241 ctgatgccgc cggcggccac caccgcgttg ccgcaccagc atcagccgga aatgccgctg
     3301 cgcaagatca ccatcaacgg cgagaagcgt cactatgccg acatgctgat ctggatttca
     3361 ccggccactc tgatgggtct gccggccacg agcgcgccgg tgggtgtgac gtcggacaat
     3421 ttgccggtga atgtgcaggt ggtgggcgcg ccgttccagg ataaaaccac catcaaattt
     3481 gcggcgttga tggcgaaggt ggtgggtggg tttgtggtgc cgccggggta ttaagtgaga
     3541 gaaatttaat cggacgcaca gcgcgcaggt ggcgggtaaa ggtttccgga acgtcagcgg
     3601 ccatggatgg ccgctgtcga gcctacaggg atgtacttgt ggcgtgtccg gaaaccttta
     3661 cccgccac
//
LOCUS       DASUEG010000584         4690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_165931, whole genome shotgun sequence.
ACCESSION   DASUEG010000584 DASUEG010000000
VERSION     DASUEG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165931"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFX11_10930"
     CDS             complement(<1..221)
                     /locus_tag="VFX11_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939182.1"
                     /translation="MSQSLPLPPGPTESKFKQAMKMGTGIYDYLSECHAKYGDAFTLR
                     FPGMDPLVWVASPEMAKDFFNLKPEQIDQR"
     gene            410..1627
                     /locus_tag="VFX11_10935"
     CDS             410..1627
                     /locus_tag="VFX11_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX4939183.1"
                     /translation="MYDIILWGATGFTGRQAARYLHEQYGRKGLIRWAIAGRDRSRLE
                     AIRAAIGAPQLDLFIVPGSDVAAADQLAQATKLVCSTVAPAADYATEMVAACVRHGTD
                     YCDLSGELHWLRAMIDRYDDAAKASGARILNACGFDSIPSDLGVQFLQEQAMNRYGEY
                     CHHIRNCFDQGHIAVSGGSFASGLGVMRAVAGDPQLANLIADPNSLNPRDRMRGACKP
                     ELDRVVHDAEFGQYLMPFPIGGINTRIVRRSHALTNFQYGEDFIYEEAKLAGNGLLRK
                     TRAQLENFFTQLFVAGDPAAPFTRFLHGLGPKVGDGPSDAQIARNGPFSFRMIGRTPS
                     GKQLRGQVYCARDPGHGGTAAMLGDTAYCLAMERDKTGRTGGFTTTSVALGPVLRERL
                     RQHAGVEFTLDSR"
     gene            complement(1654..2199)
                     /locus_tag="VFX11_10940"
     CDS             complement(1654..2199)
                     /locus_tag="VFX11_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4939184.1"
                     /translation="MLKKILISVAVLFAVVIAGVLGFATTQPDSFRVERSTRINAPAS
                     VIFPFINDLQRWSVWSPYEKIDPALKRTFSGAASGTGAIYDWDGNNEIGAGRMEIVAE
                     TPPSQLSIQLDFLRPMEGRNQVEFTLNEAGGSTLVTWSIQGPLPFLSKVMCLFFDMDR
                     MIGKDYEAGLAALKKVSEAGV"
     gene            complement(2221..2589)
                     /locus_tag="VFX11_10945"
     CDS             complement(2221..2589)
                     /locus_tag="VFX11_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4939185.1"
                     /translation="MNYLCLVYYDEKIMTSMSPREWDDLNQECIACGEGLRESGHWLG
                     GNALQPVETATTVRVRNGKITTTDGPFAETREQLAGFYLLQARDLNEAITLAGKIPPA
                     RYGSIEIRPVRELPPKRNDD"
     gene            complement(2612..3442)
                     /locus_tag="VFX11_10950"
     CDS             complement(2612..3442)
                     /locus_tag="VFX11_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008485837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4939186.1"
                     /translation="MRFMIIRKADADTEAGVMPGNDLLSAMARYNQSLIEAGMMLDGM
                     GLHPSDKGARIQFKNGKPVITDGPFAETRELIAGFTLIQAQSKEEALDWVKRWPAEDA
                     NGNAYLELRRVYEMEDFAAGDGIEQHQQNAAQLAAQLSTQQLRLNPYLNFNGNCRVAF
                     EFYEKVLGGRIENLMTFGESPMCSEVPKELHHRVIHVRLNLGNAVLMGSDAPPHRFQP
                     NQGIANNLQVPDPAEAERIFNALADNGTIVMPLATTFWARRFGALTDQFGIQWMINCE
                     "
     gene            3694..4518
                     /gene="ppk2"
                     /locus_tag="VFX11_10955"
     CDS             3694..4518
                     /gene="ppk2"
                     /locus_tag="VFX11_10955"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX4939187.1"
                     /translation="MAKKNKQQEDVVPAETERGPLSNKEYEKELKRLHVELVKLQQWV
                     VHKGLKVCIVFEGRDGAGKGGTIKAITERVSPRIFRVVALPAPTEREKTQMYVQRYLG
                     HLPAAGEVVIFDRSWYNRAGVERVMGFCTPEQTQKFLSAVPMVERAMVESGIILVKYW
                     LEVSPEEQERRLHDRIHDGRKIWKLSPMDVKSYNRWDDYTQARDDMFTHTDTAWAPWY
                     VARSEDKKRVRLNVISHLLSQIPYEEVKTEKVKLPKRKIGRYKAIDYPFKYVDEKF"
BASE COUNT          917 a         1334 c         1457 g          982 t
ORIGIN      
        1 cgctggtcga tctgctcggg cttcagattg aaaaaatcct tcgccatttc ggggctggcc
       61 acccacacca gcgggtccat gcccgggaag cgcagggtga aggcgtcgcc gtatttggcg
      121 tggcattccg acagatagtc gtaaatgccg gtgcccattt tcatggcctg tttgaatttg
      181 gattcggtgg ggcccggtgg caggggcagg gattggctca tggatgaagt cctctgtaac
      241 cggcgcacac agcggtgtgc cgttcatttt tattttgggt ggtcagggtc gggttggagc
      301 ggtgtaactt tgtatacagt gtatacccag ttcgatatac tccacaacag ccaataaaac
      361 ccactgtttt acttgggaat aatcgacaca taacaaaaag gggcaggtca tgtacgacat
      421 cattctgtgg ggtgccaccg gtttcaccgg acgccaggcc gcccgctatc tgcacgagca
      481 atacggcagg aaagggctga tccgctgggc gatcgcgggg cgggaccgct cccggctgga
      541 agcgattcgt gccgccatcg gcgcgccgca acttgacctc ttcatcgtgc cgggcagtga
      601 cgtggccgcc gccgaccagc tggcgcaggc gacaaaactg gtgtgttcca ccgtggcccc
      661 tgcggcagac tatgcgacgg aaatggtggc cgcgtgtgta cgtcacggta cggattactg
      721 cgatctgagc ggcgaactgc actggctgcg ggcgatgatc gaccgctacg acgatgcggc
      781 gaaagccagc ggtgcgcgca tcctgaatgc ctgcggtttt gattccattc cctcggacct
      841 gggcgtgcag ttcctgcagg aacaggccat gaaccgctac ggcgaatatt gtcaccacat
      901 ccgcaactgc tttgaccagg gccatatcgc ggtgtccggc ggcagttttg ccagtggcct
      961 gggggtgatg cgggcggtgg cgggtgatcc gcaactggcg aacctgatcg ccgatcccaa
     1021 cagcctgaat ccccgtgatc gcatgcgcgg cgcctgcaag ccggaactgg accgcgtggt
     1081 gcacgatgcc gagttcggcc agtacctgat gccgtttccg attggcggca tcaatacgcg
     1141 gatcgtgcgg cgttcccatg cgctgacgaa ttttcagtac ggcgaggatt tcatttacga
     1201 ggaagcgaaa ctggcaggca acggattgct cagaaaaacc cgcgcccagc tggaaaattt
     1261 tttcacgcaa ttgtttgtag ccggcgatcc ggccgcaccg ttcacccgtt ttctgcacgg
     1321 gctgggtccc aaggtgggtg acggccccag cgacgcgcag atcgcccgca acgggccgtt
     1381 cagttttcgc atgatcgggc gcacgccgtc gggcaaacaa ttgcgggggc aggtgtactg
     1441 cgcccgcgac ccgggccacg gtggcacggc tgccatgctg ggggatacgg cgtattgcct
     1501 ggcgatggag cgggacaaaa ccggtcgcac cggcggcttc accaccacca gcgtggcgct
     1561 ggggccggtg ctgcgggaac ggttgcggca acatgcgggt gtggagttca cgctggattc
     1621 ccgctaacca ttccgcgtca ggagggcgtc gggtcagaca ccagcctcac tcactttttt
     1681 cagcgcggcc agccccgcct cgtaatcctt gccgatcatc ctgtccatat cgaagaacag
     1741 gcacatcact ttggacagaa acggcagggg gccctgaatg gaccaggtga ccagcgtgct
     1801 gccgccggct tcattcagcg tgaattccac ctggttgcga ccttccatcg gtcgcaggaa
     1861 atccagctgg atactgagtt gggacggtgg cgtttcagcc acgatttcca tgcggccggc
     1921 gccgatttcg ttgttgccgt cccaatcgta gatggcgccg gtaccactgg cagcaccgct
     1981 gaacgtccgc ttcagggcgg gatcgatttt ttcatagggc gaccagacgc tccagcgttg
     2041 aagatcgttg atgaagggaa aaataacgct ggccggcgcg ttaatacgcg tactgcgttc
     2101 cacccggaaa ctgtcgggtt gtgtagtggc aaagcccaac acgccggcga tcacgacagc
     2161 aaacagaaca gcgactgata tcagtatttt tttcaacatg gttggcttcc tggcaaggtt
     2221 tcaatcgtcg ttgcgtttgg gcggcagttc ccgcaccggc cggatctcga tgctgccgta
     2281 acgcgccggt ggaatttttc ccgccagtgt gatggcttca ttcagatcgc gtgcctgcag
     2341 cagataaaag cccgccagct gctcgcgggt ttccgcgaac gggccatcgg tcgtggtgat
     2401 cttgccattg cgcacccgca cggtggtggc ggtttccacg ggttgcaatg cgttgccgcc
     2461 cagccagtgg ccactttcgc gcagcccttc accgcaggcg atgcattcct gattcagatc
     2521 gtcccactcg cgcggcgaca tggaggtcat gattttttca tcgtaataaa ccaggcacag
     2581 atagttcatg gggtttgtcc tcgcaggggt gttattcgca gttgatcatc cactgaatcc
     2641 cgaactggtc tgtcagcgcg ccgaatctgc gcgcccagaa cgtggtggcc aggggcatga
     2701 cgatggtacc gttgtccgcc agtgcattga agatgcgttc ggcttcggcg gggtccggca
     2761 cttggagatt gttggcgatg ccctggttgg gctggaagcg gtggggtgga gcgtccgatc
     2821 ccatcagcac ggcattgccc agattgaggc gcacatgaat cacccgatgg tgcagttcct
     2881 tcggcacttc gctgcacatg ggcgactcgc caaacgtcat caggttttcg atcctgccgc
     2941 ccagcacttt ctcatagaat tcgaacgcga cgcggcagtt gccgttgaaa ttcaggtaag
     3001 gattgagtcg cagttgttgt gtggatagtt gcgcggccag ttgcgctgca ttttgctggt
     3061 gctgctcgat gccgtcgccg gcggcaaaat cctccatttc atacacccgg cgcaattcca
     3121 gataggcgtt gccattggca tcttcggcgg gccagcgttt cacccagtcc agcgcctctt
     3181 cttttgattg cgcctgaatc agggtgaagc cggcaatcag ttcgcgggtt tcggcgaagg
     3241 ggccgtcggt aatgacgggc ttgccgttct tgaactggat gcgggcaccc ttgtcggatg
     3301 gatgcagacc catgccgtcc agcatcatgc cggcctcgat cagggattga ttgtaccggg
     3361 ccatggcgga cagcaggtcg ttgccgggca tcacgcccgc ttcagtgtcg gcgtcggcct
     3421 tgcggatgat catgaaacgc atggaaattc tccttgtgag tgatcccggt gaatggtcaa
     3481 tgtgaagtgt tcattgaata gtcgctgtcg ctgtggcctt ttcgacagtc gccgccattt
     3541 tttttcactg tgcataggtt tcttcaacct gaacttttct tgatctggct gcgttgcggc
     3601 catcggcgac tgccagtatt ctgatctgca gtttcccgtc aaaggccgcc cgtccgggcg
     3661 cccccaacag cctgatcgat ggaaaggtga catatggcga agaaaaacaa gcagcaggaa
     3721 gacgtggtac cggcagaaac cgagcgcggt cccctcagca acaaggaata tgaaaaggaa
     3781 ctcaagcgcc tgcatgtgga gttggtaaaa ctgcagcaat gggtggtgca caagggcttg
     3841 aaagtgtgca tcgtcttcga agggcgcgat ggcgccggca agggcggcac catcaaggcc
     3901 atcaccgaac gggtaagccc gcgcatcttc cgcgtggtgg cactgccggc gcctaccgaa
     3961 cgggaaaaaa cccagatgta cgtgcagcgt tacctgggcc atctgccggc cgccggcgag
     4021 gtggtgattt tcgaccgcag ctggtacaac cgcgccggcg tggaacgggt gatgggtttc
     4081 tgcacgccgg aacagaccca gaaattcctc agcgcggtac ccatggtgga gcgggcgatg
     4141 gtggagtccg gtatcatcct ggtgaaatac tggctggagg tgagccccga ggaacaggag
     4201 cgccgtctgc acgaccgcat ccacgatggc cgcaagatct ggaaactgtc acccatggac
     4261 gtgaaatcct acaatcgctg ggacgactac acccaggcac gcgacgacat gttcacccac
     4321 acggataccg cctgggcgcc ctggtacgtg gcgcgttccg aggacaagaa acgggtgcgg
     4381 ctgaatgtca tcagccatct gctgagccag ataccctatg aagaagtgaa gactgaaaaa
     4441 gtgaagctgc ccaagcgcaa gattggccgc tacaaggcga tcgattatcc cttcaagtat
     4501 gtggacgaga aattctgatc ccgctgcgga tcagggggtg ggcgccgtac cggatgtggc
     4561 gcccgccggc aatgcctgat aacggtggat ggcggcacgg gtgttggaca gtatccgctc
     4621 tgctccgatc tggtcgcgca atcccgagcg gctgatcatg tccgttaccc gctcggtcat
     4681 ccccaccacc
//
LOCUS       DASUEG010000585         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_966997, whole genome shotgun sequence.
ACCESSION   DASUEG010000585 DASUEG010000000
VERSION     DASUEG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966997"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1221
                     /locus_tag="VFX11_10960"
     CDS             100..1221
                     /locus_tag="VFX11_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX4939188.1"
                     /translation="MLETILTIRRAYTAGILNQIVTTQQLKLEPADSRRLARLCGQFD
                     ENLRQIEERLDVQIKNRGNAFQLTGHPDQVATASTVLDSLYRDTFANREITPDQVHLQ
                     LSECLMDAGLSGIGTPLTSGLHDDPESGELSVRTRKGAIKPRGENQKDYVRKIFRHDI
                     NFGIGPAGTGKTYLAVACAVEALEAEKVRRILLVRPAVEAGEKLGFLPGDLAEKINPY
                     LRPLYDALYEMLGFERVAKYIDRQIIEVAPLAYMRGRTLNNSFIILDESQNTTREQMK
                     MFLTRIGFGSKAVITGDYTQIDLPRGTQSGLRHAMDVLQGIDEIGFTHFDSKDVVRHT
                     LVQRIVEAYDHFETTEEKDRQEKRIQREREYRNREQPAQ"
     gene            1218..1706
                     /gene="ybeY"
                     /locus_tag="VFX11_10965"
     CDS             1218..1706
                     /gene="ybeY"
                     /locus_tag="VFX11_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX4939189.1"
                     /translation="MSAPAALELDLQIACEASDLPAEADFTGWVQAALDGAGFKAPQD
                     MPTEMTIRVVDTEESRELNRTYRDKDKPTNVLSFPFDVPDDVELALLGDLVICAPVVA
                     QESQEQDKTTRAHWAHMVVHGTLHLLGFDHVEADEAAEMETLEVQILGKLGFADPYVI
                     DN"
     gene            1728..2573
                     /locus_tag="VFX11_10970"
     CDS             1728..2573
                     /locus_tag="VFX11_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX4939190.1"
                     /translation="MNDDHSRNGSSGRTFLDRIANFLSGEPTTQAEVLEILTNAHRSG
                     LIDSEALGIFQGAMQVSDMQVREIMVPRSQCVVLSATAKPEEYLPLIIESAHSRFPIY
                     SDSPDNIIGILLAKDLLDLVARNRLDKTPLKEMIRPATCVPESKRLNVLLREFRQTRT
                     HMAIVVNEYGKMSGLVTIEDVLEQIVGEIGDEHDFDDDYLIKPGKGNEFVVKAVTPVD
                     EFNEHFGTTFGEDEYDTIGGIVLGQFGRLPKRDESVNIGKYNFTILNADNRAIRLLKV
                     SPIQH"
     gene            2583..4136
                     /gene="lnt"
                     /locus_tag="VFX11_10975"
     CDS             2583..4136
                     /gene="lnt"
                     /locus_tag="VFX11_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX4939191.1"
                     /translation="MNFNRTPTAGGKGHLLALLAGAVFPLGLAPLDLWPLIPVSMAVL
                     VWLLEGLKPGQAFKRAWWYGIGFNGVGVSWVYVSIHYFGHTPLWLSVLATALFVAFLA
                     LVLFAIPFWFYRRVNLDRSALLAFPALWVLVEWSKTWILSGFPWLWAGYGFIDTPLAP
                     LGPVTGVLGISFVVALTAVLLRQLVQRHTVQQHNDQRGRAALALIVIWGASFALGQFQ
                     WVQPNAEPALKVALVQGNIDQDKKWDPAYRDLILLTYKNLTEQAWDADLILWPEAAYP
                     VFHHQASNFISNLNIRAREKNVAVVSGVPFWQWENERSTYYNSIFVIGAGDGLYHKQR
                     LVPFGEYVPLEQYIRGLIPFFNLPMSSFTPGRDDQKPLEAKGLKFAAYICYEIVYPEL
                     VRTEALGKDFLVTISNDAWFGRSWGPLQHFQMARMRALETGKYLLRGTNTGVTAVIDH
                     QGKVLGQLPQFQEGLLKGSFRTVSGVTPYMHWGWWPVLGLSGALVLIGLVLGFGPARP
                     PQERPLFRK"
BASE COUNT          854 a         1283 c         1255 g          773 t
ORIGIN      
        1 agaaaactcc ccccgccgcg ccaaatcgct ggcacccccg taaaccagcc cgacaccggc
       61 cacccaatta cggcttttcc tgctccgcgc ttccggtata tgctagaaac catcctcacc
      121 attcgcaggg cgtataccgc aggcattttg aaccagatag tgactaccca gcagctcaaa
      181 ctcgaacccg ccgattcccg ccgcctcgct cgcctgtgcg ggcaattcga cgaaaacctc
      241 cgccagatcg aagaacgcct cgacgtccag atcaagaacc ggggcaatgc cttccagctg
      301 accggacatc cggatcaggt cgccaccgcc agcaccgtac tggacagcct ctaccgcgac
      361 accttcgcca accgtgaaat cacccccgac caggtgcacc tgcaactgag cgagtgcctg
      421 atggatgcag ggctcagcgg cattggcacc ccactcacct ccggcctgca cgatgatccg
      481 gaatccggcg aactgagcgt gcgtacccgc aagggtgcca tcaagccccg gggcgaaaac
      541 cagaaggatt acgtccgcaa gatcttccgc cacgacatca acttcggcat cggtcccgcc
      601 ggtaccggca agacctacct ggcggtagcc tgcgcggtgg aagcactgga ggcggaaaag
      661 gtgcgccgca tcctgctggt gcgcccggcg gtggaagcgg gtgaaaaact gggcttcctg
      721 cccggcgacc tggcggaaaa gatcaatccc tacctgcgtc cgctatacga cgcgctgtat
      781 gaaatgctcg gcttcgagcg ggtcgccaag tacatcgacc gccagatcat cgaagtggcc
      841 ccgctggcct acatgcgcgg tcgcaccctt aataattcct tcatcatcct ggatgaaagt
      901 cagaacacca cccgggagca gatgaaaatg ttcctgaccc ggatcggttt cggttccaag
      961 gctgtcatca ccggcgacta cacccagatc gacctgcccc ggggcaccca gtccggtctc
     1021 aggcacgcca tggatgtgtt gcagggcatc gacgaaatcg gcttcaccca tttcgactcc
     1081 aaggacgtgg tgcgccacac cctggtgcag cgcatcgtcg aagcctacga ccatttcgaa
     1141 accacggaag agaaggatcg ccaggaaaag cggatacagc gcgagcgcga ataccgcaac
     1201 cgggaacagc cggcgcaatg agcgcgcctg ccgccctgga gctggacctc cagattgcat
     1261 gcgaagcatc cgaccttccc gccgaagccg atttcacggg ctgggtgcag gccgcacttg
     1321 acggcgccgg cttcaaggcg ccgcaggata tgcccaccga aatgaccatc cgggtggtcg
     1381 ataccgaaga aagtcgcgaa ctgaaccgca cctatcgcga caaggacaag ccgaccaatg
     1441 tgctgtcgtt tccgtttgac gtaccggatg atgttgaact ggccctgctg ggagatctgg
     1501 tgatctgcgc accggtggtg gcacaagaat cgcaagaaca ggacaagacc acccgggccc
     1561 actgggccca catggtggta cacggcacgc tgcatctgct ggggttcgat catgtcgaag
     1621 cggacgaagc ggccgaaatg gagacgctgg aggtgcagat cctgggcaaa ctgggctttg
     1681 cagaccccta cgtaatcgat aactaaaatg gagtgacacg cgtcatcatg aacgacgacc
     1741 acagtcgtaa tggttcatcg ggccgaacct ttctcgacag aatcgcgaat tttctttccg
     1801 gtgagccaac cacccaggcc gaagttctgg aaatcctgac caacgcccac cgctcggggc
     1861 tgatcgattc cgaagcgctg ggaatcttcc agggcgccat gcaggtgtcc gacatgcagg
     1921 tgcgggagat catggtgcca cgctcgcagt gcgtggtgct gtccgccacg gcgaagccgg
     1981 aagaatacct gcctctgatc atcgaatccg cccactcccg tttccccatc tacagtgatt
     2041 cacccgacaa catcatcggc atcctgctgg cgaaagacct gctggatctg gtggcccgca
     2101 accgcctgga caagaccccg ctgaaagaga tgatccgccc cgccacctgc gtgcccgaaa
     2161 gcaagcgcct gaatgtgctg ctgcgggaat tccgccagac ccgcacccac atggcaatcg
     2221 tggtaaacga atatggcaag atgtcgggcc tggtgacgat cgaggacgtg ctggagcaga
     2281 tcgtgggcga gatcggcgac gagcacgatt tcgacgatga ctacctgatc aagccgggca
     2341 agggcaatga attcgtggtg aaggcggtga cgccggtgga cgagttcaac gagcacttcg
     2401 gcacaacctt cggcgaggac gaatacgaca cgattggcgg aattgtgctg ggtcagttcg
     2461 gacgactgcc gaaacgggat gaaagtgtca acatcggcaa gtacaatttc accatcctca
     2521 acgccgacaa tcgggcgatc cgtctgctga aagtcagccc gattcaacac taagaccttt
     2581 tcatgaattt caatcgtacc ccgactgcgg gtggcaaagg ccacctgctg gccctgctcg
     2641 caggcgctgt tttccctttg ggcctggcgc cgctggatct gtggccgctg attccggtgt
     2701 ccatggccgt gctggtctgg ctgctggagg gcctcaagcc cgggcaggcg ttcaagcgcg
     2761 cctggtggta tggcatcggg ttcaatgggg tgggcgtatc ctgggtctat gtgtcgatcc
     2821 attatttcgg tcacacgccc ctgtggctgt ccgtgctggc caccgcgctg ttcgtggcat
     2881 ttctggcgct ggtgctgttt gcgattccgt tctggttcta tcgccgcgtc aatctggacc
     2941 gcagtgcact gctcgccttt cccgcgctgt gggtgctggt ggaatggagc aagacctgga
     3001 tactgagcgg cttcccctgg ctgtgggcgg gctatggatt tatcgacacg ccgctggcgc
     3061 cgctgggccc ggtcaccggc gtgctgggca tcagcttcgt ggtggcgctg acggcagtgc
     3121 tgctgcgtca gctggtgcag cgccacactg tccagcaaca caatgaccag cgcggccggg
     3181 ctgcgctggc gctgatcgtg atctggggcg ccagtttcgc cctggggcaa ttccagtggg
     3241 ttcagcccaa tgccgaaccg gcgctgaagg tggcactggt gcagggcaac atcgatcagg
     3301 acaagaagtg ggacccggcc taccgcgacc tgatcctgct cacctacaag aatctgacgg
     3361 agcaggcctg ggatgcagat ctgatcctgt ggccggaagc ggcttacccg gtgttccatc
     3421 accaggcttc caatttcatt tccaacctga atatccgcgc ccgggaaaag aacgtggccg
     3481 tggtgagtgg cgtgccgttc tggcagtggg aaaacgagcg cagcacttac tacaactcca
     3541 ttttcgtgat cggcgccggc gacgggctgt atcacaagca gcggctggtg ccattcggcg
     3601 agtacgtgcc gctggaacag tacatccgcg gcctgattcc gtttttcaac ctgcccatgt
     3661 ccagctttac gccagggcgc gacgaccaga agccgctgga agcgaaaggg ctgaaattcg
     3721 ccgcctatat ctgctatgag atcgtgtatc cggagctggt gcgcaccgag gcgctgggca
     3781 aggattttct ggtcaccatc agcaacgacg cctggttcgg ccggtcctgg ggcccgctgc
     3841 agcatttcca gatggcgcgc atgcgggcgc tggaaaccgg caaatatctg ctgcggggca
     3901 ccaataccgg cgtgaccgcg gtgatcgatc accagggcaa ggtgctggga caactgccgc
     3961 agtttcagga aggcctgctc aaaggcagct tccgcaccgt cagtggggtg acgccctaca
     4021 tgcactgggg ctggtggccg gtgctcggac tgtcgggagc gctggtgctt atcgggctgg
     4081 tgctgggctt tggtccggcg cgacctccgc aggagcggcc tttgtttcgg aagtagcggt
     4141 tccggaaaca gtggatgcag ccacc
//
LOCUS       DASUEG010000586         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_56294, whole genome shotgun sequence.
ACCESSION   DASUEG010000586 DASUEG010000000
VERSION     DASUEG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56294"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VFX11_10980"
     CDS             complement(<1..1752)
                     /locus_tag="VFX11_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX4939192.1"
                     /translation="MNRAVSGLAFAVAMASSGFANAYDYQLRQNINITSADGTHISAH
                     AYVPVGAPGEKFPVIMFVNSWGLGESEYDKQAMKFAEQGYVALAYAARGWYTSGGLID
                     VAGPKDMQDVNAVVDYLVAMPEVDDDNIGMTGVSYGGGMTLLAAAHEPRIKTVVSFSG
                     WTNLMDSLYGGMPGDANGQGQSTPRTTWASLLIFAGNLIGHMSSDVEENWDRLMNNYE
                     IDQVTDWAAQRSAATYIDEYNQRQIPVYMINNLQDQLFQSNQIEKFYSGLNYDAKRFD
                     VYRGIHITGEIGGFLNVAGTLADFLGQGEIRSAIVSIVNALGINFGHMDNNVPWKNAH
                     LWFDHYLKGVAVDPATGRQIAAEEKVNLKDKVASATFKDWAAPQDSAGVTEQTFYFDR
                     SQDAWYLPVTNKLKTQTVGSSNYSIYTGQDTTATTGINLPGNLDSFVDIITSFLLDDI
                     TLSSTLGGLMEAHLPNTYRTADLGNFTKYYLNINCPIGCYREAGVRYDTTTFSAAKNI
                     VGTAHMNLWVESQSAELHLTTYVYEVLPNGPSRLITDGPVTRRNVVTNQPINLDFDMV
                     FTNYTTQPGSKIAVVFDT"
     gene            2571..2951
                     /locus_tag="VFX11_10985"
     CDS             2571..2951
                     /locus_tag="VFX11_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939193.1"
                     /translation="MFAQNENRRSFLYPGQPGAFLFYCGVTLCVALGVALLKNSLENR
                     LLRAQLEMSRFECRDEPATTQLSAAGKSPVTFSVSAGMPDTAKPPRQWVPLTTGVLST
                     AAGQAATGYSATGPQCLIWTEGNT"
     gene            3127..4149
                     /locus_tag="VFX11_10990"
     CDS             3127..4149
                     /locus_tag="VFX11_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939194.1"
                     /translation="MDLGHFSARYVATIVTVAELQGLDRNWLLQQLALDEAYLKLSIN
                     RVPSIRVIELLHTIIAATGQTCFGLMVGDSFKPGTFDILGLAAMCSNTLREAVLLNHR
                     YQPLNQTLGVSRLEEQGTHAAVVWTPRQQDPEHLRPITEAVMAGYAGIGRWITWHAQQ
                     PLLKMEFRHRAPQLTQEYERIFGCEIEFGAARDAILVERSFLDTPLQQADEVTVRVLR
                     RQLDFRLQSLARYESVRHQLAGYLQSQLAREIPSLQRASRALGCSERTLRRRLQQENT
                     SFREILDDVRRICALEYLQRGELELADIAQLLGYQDQSTFTHAFRNWFDVTPGIYRKS
                     LTGGRP"
     gene            complement(4159..>4343)
                     /locus_tag="VFX11_10995"
     CDS             complement(4159..>4343)
                     /locus_tag="VFX11_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4939195.1"
                     /translation="FKELVDSVRRVLVLHYLRDSQTSLQDTAFLLGFSEQSAFSRAFR
                     RWQGCTPQQYRKQQRD"
BASE COUNT          929 a         1231 c         1242 g          941 t
ORIGIN      
        1 ggtatcgaac accacggcga tcttgctgcc aggctgggtg gtgtagttgg tgaacaccat
       61 gtcgaagtcc agattgatcg gttgattggt taccacattg cggcgggtga ccgggccgtc
      121 ggtgatcaac cggctcgggc cattgggcag cacttcatac acatacgtcg tcaggtgcag
      181 ctcagcggat tgtgattcca cccacaggtt catgtgcgca gtacccacga tatttttcgc
      241 agcgctgaat gtggtggtgt cgtagcgcac acccgcttcg cggtagcaac caatcgggca
      301 attgatattg aggtagtact tggtgaaatt gcccagatcg gcagtgcgat acgtgttggg
      361 gagatgcgct tccatcaaac cgcccaatgt ggacgacagc gtgatgtcat ccagcagaaa
      421 gctggtaatg atatcgacga aactgtccag attaccgggc agattgatgc cggtggtggc
      481 cgtcgtgtcc tgtccggtgt agatggagta gttactgctg ccgacggttt gggttttcag
      541 tttgttcgtc accggcaggt accaggcatc ctgcgaccga tcgaagtaaa aggtttgctc
      601 ggtcaccccg gcggaatcct gcggcgctgc ccagtccttg aatgtggcgc tggccacctt
      661 gtctttcaga ttaacttttt cttcggcggc gatctgcctg ccggtggccg gatccactgc
      721 gacgcctttc agatagtggt cgaaccacag gtgagcattc ttccagggaa cgttattgtc
      781 catatggccg aagttgatgc ccagcgcatt gacgatgctg acgatcgcac ttctgatctc
      841 gccctgcccc agaaaatcag ccagtgtgcc cgccacgttg aggaagccgc caatttcacc
      901 ggtgatgtga atgccgcgat agacatcgaa gcgtttggcg tcgtaattca gcccgctgta
      961 gaacttctct atctggttgg actggaacag ttggtcctgc aggttattga tcatgtacac
     1021 aggaatctga cgctggttgt attcgtcgat gtaggtggcg gcgctgcgct gggcagccca
     1081 gtccgtgacc tgatcaattt catagttgtt catcaaccgg tcccagtttt cttccacatc
     1141 cgacgacatg tggccaatca ggttgccagc aaagatcagg agcgatgccc aggtggtgcg
     1201 gggagtggat tggccctggc cgttggcgtc acccggcatg ccgccgtaca gggaatccat
     1261 cagattggtc cagccgctga aggacaccac ggtcttgata cgcggctcat gggcggcggc
     1321 cagcagtgtc atgccgccac cgtaggaaac accggtcatg ccgatgttgt cgtcatcaac
     1381 ttcgggcatg gccaccagat aatccaccac cgcgttgacg tcctgcatgt ccttgggccc
     1441 tgccacatcg atcaggccac cggacgtgta ccagccacgg gccgcatagg ccagggccac
     1501 ataaccttgc tctgcaaatt tcatggcctg tttgtcatat tcactttctc ccaggcccca
     1561 gctgttgacg aacatgatca caggaaattt ttcacccggc gcacccaccg gtacataggc
     1621 gtgtgcagaa atatgggtac cgtcggcgga ggtaatattg atgttctggc gaagctgata
     1681 gtcataggca ttggcgaagc cgctgctggc catcgcaact gcgaaagcca atccgcttac
     1741 tgctcggttc atttttttct cccggttaat cacacaaata gacaggaatg cccacgtgca
     1801 cgcatccatt gattagagtt ccgaaccacc atttgggaat ggcaatggga aggagagcaa
     1861 tggctgtacc cgatgatcac gatgtgccgg cgcagtcacg gactggaagg gaaattcagc
     1921 gcagaaaaat aaataggccg gaatattaga gagatagcga atttgctcga ttacagaaag
     1981 tagcacgcca gacacatcgt aagcccgcta taaccactgg ctatcggaaa gcgttttttc
     2041 gacgcgattt gtactcatgc gcggtaacaa tcgtcgagcc cttgacggaa cgaatcgggc
     2101 taggaacgct caatggcgag acaaattctt gttttcgtat tttttaggac acattcaact
     2161 aacggacgcc aggtgactcg cagtcttcac agttcgtaac attcaaggca tcaatccgcg
     2221 ataacccgcg cagccaagct catttttttt catatcggat tcaagtcgcc ttcaacgagt
     2281 gtggtgtaca gggaagaaaa aaggagtgac ctgttaccaa tggttgcggt gctccaggca
     2341 ccggagaggg aaacagaagc gatcaggggc tatcagtcct cctgccgttg tgcgcgcacg
     2401 tgacctgcat acgttggcgc tgtcgccggt ggaggcaatc ctcttttcaa ttcatggccg
     2461 caattcccaa accgtttggc cttcctgccc aatatgtagc agcaccacac cagtacattc
     2521 ctccaaagag agatcgcaca caaagaaagt tcgcccgagg aattcccgcc atgtttgcac
     2581 agaacgagaa tcggcgcagt ttcctgtacc ccggtcagcc cggcgctttt ctgttttatt
     2641 gcggtgtcac gctgtgcgtg gcgctgggcg tggccctgct gaagaattcc ctggaaaacc
     2701 gcctgctgcg ggcgcaactg gaaatgtccc gctttgaatg ccgtgacgaa ccggcaacaa
     2761 cgcaactcag cgcggcgggc aaatcccctg tcaccttttc ggtatctgcc ggtatgccgg
     2821 acactgccaa acccccgcgg cagtgggttc cactcaccac cggcgtcctt tcgacagcgg
     2881 caggtcaggc tgccactgga tattccgcaa ccgggccgca atgtctgatc tggacagaag
     2941 gcaacacctg aataaaccaa ccagtcatac accaatccat tccaaagcgg ccatcccgct
     3001 gtttgcgggt gccgcagccg accatgaaat cgcccgcaga gcattcttct gcgaagccgg
     3061 tctcaccttc tgcacgcagc actggtaata tgccttctcc gcatcggaac cggacgagcc
     3121 atcaccatgg atttgggtca cttttccgcc cgctacgtcg ccaccattgt caccgttgct
     3181 gaactccagg gactggatcg caactggctg ctgcaacaac tggcgctgga cgaggcctat
     3241 ctgaaactga gcatcaaccg ggtgccgtcg atccgcgtga tcgaattgct gcacaccatc
     3301 atcgcggcca ctggccagac ctgcttcgga ctgatggtgg gtgattcgtt caagccgggg
     3361 acgttcgaca ttcttggcct cgccgccatg tgcagcaata cgctgcggga agccgtgctg
     3421 ctcaatcatc gctatcagcc gctgaaccag acgctgggtg tgtcccgact ggaggaacag
     3481 ggcacgcacg cagccgtggt gtggacgccg cgccagcagg acccggaaca cctgcggccc
     3541 attaccgaag ccgttatggc cggttatgcc ggcattggcc gctggatcac ctggcatgcg
     3601 cagcagccgc tgctgaagat ggagttccgt caccgggcgc cgcaactgac gcaggaatac
     3661 gaacgcattt tcggctgcga gattgaattt ggcgcggccc gcgatgccat cctggtggag
     3721 cgttcctttc tggacacccc gctgcagcag gcggatgaag tgacagtgcg ggtgctgcgg
     3781 cggcaactgg atttccgcct gcaaagtctg gcgcgctacg aatccgtgcg tcaccagctg
     3841 gcggggtatc tgcaaagtca attggcgcgg gagattccat cgctgcaacg ggcgtcacgt
     3901 gcgctgggct gttcggagcg gacactgcgg cgacggttgc aacaggagaa cacgtcgttc
     3961 cgggaaatcc tggatgatgt gcgacggatc tgtgcgctgg aatatctgca gcgcggcgaa
     4021 ctggaactgg cggacattgc gcagctgctg ggctatcagg accagagcac cttcacccac
     4081 gcctttcgca actggtttga tgtgacgcca gggatttacc ggaaatcgct gacgggcggg
     4141 cggccctgag gggttgcgtc aatcccgctg ctgcttgcga tactgctgcg gtgtgcagcc
     4201 ctgccagcgc cggaacgcac ggctgaaggc actctgctcg gaaaagccca gtaaaaatgc
     4261 agtgtcctgc aggctggtct gcgaatcgcg cagataatgc agcaccagga cacgacgcac
     4321 gctgtccacc agttccttga agg
//
LOCUS       DASUEG010000587         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2102601, whole genome shotgun sequence.
ACCESSION   DASUEG010000587 DASUEG010000000
VERSION     DASUEG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102601"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..366)
                     /locus_tag="VFX11_11000"
     CDS             complement(28..366)
                     /locus_tag="VFX11_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX4939196.1"
                     /translation="MALLLNFAVITYLALTSVSIKPAQLTSDKVNHCFAFFVLAFCID
                     YAFPRLRFLLVKIWPLLAYGIAIEIVQRFVARDASLLDFGADLLGITLYWLPRRYLRR
                     LVTGRDFAAA"
     gene            complement(566..805)
                     /gene="nqrM"
                     /locus_tag="VFX11_11005"
     CDS             complement(566..805)
                     /gene="nqrM"
                     /locus_tag="VFX11_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEX4939197.1"
                     /translation="MATFLLTTLLFLVIFAAMAVGVIFSNKPIKGSCGGLNNVGLGGE
                     CEICGGNLDQCEAAQGDRKASPAARDALGYDATRR"
     gene            complement(841..1869)
                     /locus_tag="VFX11_11010"
     CDS             complement(841..1869)
                     /locus_tag="VFX11_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX4939198.1"
                     /translation="MRFRTFWLGVFWCAVLLAGCSSPAPEILEFSGESMGTTWSVKVS
                     AMPAGLKPPQVADHIQAALDGVNQSMSTYLATSELSRFNQAPAGTRMQVSPALFDVMR
                     KSVEVWRLSQGTFDVTVGPLVNLWGFGPQGRPNVVPDEAAQREAWARVGTDALTLHTE
                     TLELEKHKDLYVDLSAIAKGYGVDRVAEALENLGVRRYLVEVGGEMRAGEPKAPGQPW
                     RVAVEKPWNGARSVERVFAVSGVAMATSGDYRNYFEVNGKRYSHTIDPRTGRPIDHAL
                     ASATVIAPTCAEADAWATAMMVLGPDQALAVARANHLAVYLLVKAGDGFEARSSEAFA
                     MYLKPVLQ"
     gene            complement(1871..3100)
                     /gene="nqrF"
                     /locus_tag="VFX11_11015"
     CDS             complement(1871..3100)
                     /gene="nqrF"
                     /locus_tag="VFX11_11015"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEX4939199.1"
                     /translation="MDNTIFLGVGMFTGIVLALVVLILLARARLDSTGDVHIDINEDP
                     ERAIDVPAGGKLLTTLADQGIFVSSACGGGGTCAQCRVIVHEGGGDILPTEEGHFSTR
                     EIKEGWRLSCQVAVKQNMKIELDEAFFGVKKWVTTVRSNHNVATFIKELVVDLPEGEV
                     VHFRAGGYVQLECPPHHVKYSNFEIEPEYRADWERFGFFKLESKVSDSVIRAYSMANY
                     PDELGLLKFNIRIATPPPGSQGIPPGQMSSWVFNLKPGDKVTVYGPFGEFFATNNDTE
                     MVFVGGGAGMAPMRSHIFDQLKRLKTKRKITFWYGARSLRELFYKEDFDALQAENDNF
                     EWHIALSDPQPEDHWNGLTGFIHNVLFEQYLRDHPAPEDCEFYMCGPPMMTAAVIRLL
                     DDLGVERDNIYLDDFGG"
     gene            complement(3122..3733)
                     /gene="nqrE"
                     /locus_tag="VFX11_11020"
     CDS             complement(3122..3733)
                     /gene="nqrE"
                     /locus_tag="VFX11_11020"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEX4939200.1"
                     /translation="MFEHYLSLLVKSIFVENMALAFFLGMCTFIAVSKKMKTAIGLGV
                     AVTFVQAITVPANNLILNYLLKEGALEWAGLGDVDLTFLGLLSYIGMIAAIVQILEMF
                     LDKYVPSLYNALGVFLPLITVNCAILGGALFMVERDYTFGESVVYGIGSGAGWAIAIA
                     VLAGIREKLKYSDVPEGLRGMGITFITVGLMSLAFMSFSGIQL"
     gene            complement(3746..>4220)
                     /locus_tag="VFX11_11025"
     CDS             complement(3746..>4220)
                     /locus_tag="VFX11_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEX4939201.1"
                     /translation="PSSIRIIVQMTIIASLVIVVDQFLRAYAYAISKQMSVFVGLIIT
                     NCIVMGRAEAYAMKNPPVASFLDGFGNGLGYSVILLILGVFRELFGSGKLLGIEIFKL
                     VSDGGWYVPNGLLLLPPSAFFLIGLLIWAMRTWKPEQVERNEYPLATHTKAQPAH"
BASE COUNT          946 a         1347 c         1164 g          763 t
ORIGIN      
        1 tgccggccct gttcgaataa accagcgtca ggccgccgca aaatcccgcc ccgtcaccag
       61 ccgccgcaga tagcgtcgtg gcaaccagta caaggtaatg cccagcagat ccgccccgaa
      121 atccagcagg gacgcatccc gcgccacgaa ccgctgtacg atctcgatcg caatgccata
      181 ggccagcaat ggccagatct tgacgagcag gaagcgcaac cggggaaagg cgtagtcaat
      241 acagaacgcc agcacgaaaa aggcgaaaca atgattgacc ttgtcgctcg tcagctgggc
      301 cggtttgatg gagacgctgg tcagcgccag ataggtgatc actgcaaagt tcagcagcag
      361 cgccatacga aaaagccaga ccagctggct ttgggaaatc ggcaatcgaa tcattgaaaa
      421 aggttcacca gataaaagac ccatcgccat ccagccccgg cagcatggcg cggaaacgga
      481 cagtcaacgc aggtgttgat gcactatgga ttccgcaagc gggcaaaaat tccccgccca
      541 atcgcatttt tcctgagcac atgattcagc gacgggtcgc gtcgtaaccc agcgcgtcgc
      601 gggcggcggg gctggctttg cgatccccct gcgctgcctc gcactggtcg aggttgcccc
      661 cgcaaatctc gcactcgcca cccagcccca cattgttcag gccaccgcag gaacccttga
      721 tgggtttatt ggagaaaatc acacccacgg ccatggcggc aaatatcacc agaaacaaca
      781 gggtagtaag cagaaaggtt gccattgcac agactcttgc gcttggaaaa caggatcagt
      841 ctactgcaga acaggtttca gatacatggc gaacgcctcg ctggatcggg cctcgaaacc
      901 gtcaccggct ttcaccagca gatacaccgc cagatggttg gcccgggcca ccgccagcgc
      961 ctgatcgggc cccagcacca tcatggcggt agcccaggcg tccgcctcgg cgcaggtggg
     1021 cgcaatgaca gtggcggacg ccagggcatg atcgatcggc ctgccggtac gaggatcgat
     1081 ggtgtgggaa tagcgtttgc cattcacctc gaaataatta cggtaatcac cggaagtggc
     1141 cattgccacg ccgctgacgg caaacacccg ctccaccgag cgcgccccgt tccagggctt
     1201 ttccacggcc acgcgccagg gctgccccgg tgccttgggt tcgccggccc gcatttcgcc
     1261 acccacttcc accagatagc ggcgaacacc cagattttcc agggcctcgg ccacccggtc
     1321 cacaccatag cccttggcaa tggcagagag atcgacatac agatccttgt gtttttccag
     1381 ttccagcgtc tcggtgtgca gggtcagggc atcggttccc acccgggccc aggcctcgcg
     1441 ctgggccgcc tcatcgggca cgacattggg ccttccctgc gggccgaatc cccacagatt
     1501 caccagcggc cccacggtga catcgaacgt gccctggctc agccgccaga cctccacgga
     1561 tttgcgcatg acgtcaaaca gcgcgggaga aacctgcatc cgcgtgccgg cgggcgcctg
     1621 gttgaagcgc gacagttcgg acgtggcaag gtaggtggac atggactggt tcaccccgtc
     1681 cagtgccgcc tggatgtggt cggccacctg cggcggtttg agtccggcgg gcatcgcact
     1741 caccttcacc gaccaggtgg tgcccatgct ttcgccactg aattcgagga tctcgggtgc
     1801 aggggacgag cagccggcca acagtacagc gcaccagaac acgccgagcc agaaggtccg
     1861 gaatctcacg tcagccgccg aaatcgtcga gatagatgtt gtctcgctcc acgcccagat
     1921 cgtccagcaa ccggatcacc gccgccgtca tcatgggcgg accgcacatg taaaactcgc
     1981 aatcttccgg cgccgggtga tcccgcaggt actgctcgaa cagcacgttg tgaataaagc
     2041 cggtaaggcc gttccagtga tcttccggct gcggatcaga aagtgcgatg tgccattcga
     2101 agttatcgtt ctccgcctgc agcgcgtcga aatcctcctt gtagaacagc tcccgcaggc
     2161 tgcgggcgcc gtaccagaag gtgatcttgc gcttggtctt gagccgcttg agctggtcga
     2221 aaatgtgcga gcgcatcggc gccatgcccg caccgccacc gacaaacacc atttcggtgt
     2281 cgttattcgt ggcaaagaat tcgccgaacg ggccgtatac cgttaccttg tcgcccggct
     2341 tcaggttgaa aacccaggac gacatctgcc caggcggaat gccctgcgaa ccaggtgggg
     2401 gcgtggcgat gcggatgttg aacttcagca gaccgagttc atcaggataa ttcgccatgg
     2461 aataggcacg gatcacgctg tcgctgactt tcgattccag tttgaaaaaa ccgaaacgct
     2521 cccagtccgc acggtactca ggctcgattt caaaattgct gtatttgacg tgatggggcg
     2581 ggcattccag ctgcacataa ccgccggcgc ggaaatgcac cacctcgcct tccggcaggt
     2641 ccaccaccag ttccttgatg aaggtggcca cgttgtgatt cgagcgcacg gtggtgaccc
     2701 atttcttcac gccaaagaag gcttcgtcca gctcgatctt catgttctgc ttcacggcca
     2761 cctggcagga caggcgccag ccttccttga tttcccgcgt cgaaaaatgg ccttcttcgg
     2821 tgggcaggat gtcgccgccg ccttcatgca cgatcacccg gcactgggcg caggtaccac
     2881 caccaccgca ggcggaagaa acaaaaatac cctggtctgc cagcgtggtg agcagcttgc
     2941 cgcctgccgg cacatcaatg gcccgctccg gatcttcatt gatgtcgatg tggacgtcgc
     3001 cggtgctgtc cagccgcgcg cgcgccagca ggatcagcac caccagcgcc agcacaatgc
     3061 cggtgaacat gccgacgccg aggaaaatcg tattatccat ctgcctgact ccccgccccg
     3121 gttacagctg aatgccggaa aacgacatga atgccagtga catcagaccc accgtgatga
     3181 aggtgatgcc catgccacgc aggccttccg gcacgtcgct gtatttgagt ttctcgcgaa
     3241 tgcccgccag caccgcaatg gcaatcgccc agccggcacc ggaaccgatg ccatacacca
     3301 cgctttcacc gaacgtgtaa tcacgctcca ccatgaacag cgcgccaccc agaattgcgc
     3361 agttcaccgt gatcagcggc aggaacacgc ccaatgcgtt gtacagcgac ggcacgtatt
     3421 tatcgaggaa catttccagg atctgcacga tggcggcaat catgccgatg taactcagca
     3481 gcccgaggaa cgtgagatcc acatcgccca acccggccca ttccagcgca ccttccttca
     3541 gcaggtaatt caggatcagg ttgttggccg gcaccgtgat cgcctgcacg aatgtcaccg
     3601 cgacgcccag tccaatcgcc gtcttcattt ttttggaaac ggcgatgaag gtgcacatgc
     3661 ccaggaagaa cgccagcgcc atgttctcga cgaaaatgga tttcaccagc aggctcagat
     3721 aatgctcgaa catgatcgtg cctccttaat gcgccggctg cgctttggta tgcgtcgcca
     3781 gcggatactc gttgcgttcc acctgctccg gcttccaggt gcgcatggcc cagatcagca
     3841 ggccaatcag gaagaacgca ctgggcggca gcagcagcaa gccattcggc acataccagc
     3901 caccatcact caccagtttg aagatttcga tgcccagcaa cttgcccgaa ccgaacagtt
     3961 cacggaacac acccaggatc agcaggatca cgctgtaacc cagaccattg ccgaaaccgt
     4021 cgagaaaact cgccaccggc ggattcttca tggcgtacgc ttcggcgcgg cccatcacga
     4081 tacagttggt gatgatcaag cccacgaaca ccgacatctg cttgctgatg gcataggcat
     4141 aggcacgcag gaactggtcc accacaataa ccagcgaggc aatgatcgtc atctgcacga
     4201 tgatgcggat actggatgga
//
LOCUS       DASUEG010000588         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_956309, whole genome shotgun sequence.
ACCESSION   DASUEG010000588 DASUEG010000000
VERSION     DASUEG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956309"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFX11_11030"
     CDS             <1..525
                     /locus_tag="VFX11_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939202.1"
                     /translation="LVNDLLLLSRLENDSVPQEPQRVNVTTLLGQIRNEAIAFGLEKH
                     HDITLECDTTVGLMGLESNLRSAFSNLVTNAVKYTPAHGRIRIRWWADDKHAYFSVSD
                     NGIGIDARHLPRLTERFYRADAARSTATGGTGLGLAIVKHVLLQHHARLDIESTLGKG
                     STFTCVFPATIVVR"
     gene            558..1322
                     /locus_tag="VFX11_11035"
     CDS             558..1322
                     /locus_tag="VFX11_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4939203.1"
                     /translation="MTLRLPLLCLLLAFAASGQAKEPLTLQLCQSNEGDYPWTLPDRP
                     GLNNLLIERAARRTGVAVRFHPMPWARCLFKTSAGEMDGVVGASYRPERLRIGVYPLK
                     DGQPHTERRLMRNSYLLYRRKGDPRVHWDGQRLQVNGVVGVQTGFSVAGTLQRLGAAV
                     DQQHRLPRPLLQQLLSGRLQAIALTAAEGQRLLAEHPQGSTVEALPAPLEEKDFYLVF
                     SRQFQRQHPALVERLWQALWLEQQTPEWRTESGRFR"
     gene            1499..>2412
                     /locus_tag="VFX11_11040"
     CDS             1499..>2412
                     /locus_tag="VFX11_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939204.1"
                     /translation="MMDRPLLISSLLEFAVVNHPQAEIVTRRVEGDIHRYTWMDAAAR
                     SARLANALNRLGVKTGDRIGTLAWNTYRHLELYFGVSGMGAVLHTINPRLFPEQIVWI
                     ANHAEDRFLFVDLTFLPLVDATRAHLKSLEKVIVLADKAAMPAGRDDLVCYEDLLAPE
                     SDSFAWPALDERTAASMCYTSGTTGNPKGVLYTHRSTVLHAMGSLHSGALSIGRDTVL
                     LPVVPMFHVNAWGTPYSAAMSGAKIVFPGAGLDGKNLHELIDKEQATLLLGVPTVWLG
                     LLNYLDSIGAKMHSVNHVVVGGSAAPLA"
BASE COUNT          446 a          841 c          697 g          428 t
ORIGIN      
        1 ctggtcaacg acctgctgct gctctcgcgc ctggaaaacg acagcgtgcc gcaggaaccg
       61 caacgcgtga atgtcaccac actgctgggc cagatacgca acgaggccat cgccttcggc
      121 ctggagaagc accacgacat cacgctggaa tgtgacacca ccgtgggact gatgggactg
      181 gaaagcaacc tgcgcagcgc cttctccaat ctggtcacca atgccgtcaa atacaccccg
      241 gcgcacggcc gcatccgcat tcgctggtgg gccgatgaca aacatgccta cttcagcgtg
      301 agcgacaacg gcatcgggat tgatgcccgc cacctgccgc gcctgaccga gcgtttttat
      361 cgcgccgatg ccgcccgctc gacagccacc ggtggtaccg gtctgggtct ggccatcgtc
      421 aagcacgtac tgctgcaaca tcacgcgcgg ctggacattg agtcaacgct gggcaagggc
      481 tccacgttca cctgcgtctt tccggcgacg atagtggtac gctgaacgct gtcgctaatg
      541 caccacccgg tatcgccatg accctccgtc tcccgctgct ttgcctgctg ctggcttttg
      601 ccgcttctgg tcaggcgaag gagcccctca ccctgcagct ttgccagagc aacgaaggtg
      661 attatccctg gacgctgcca gaccgccccg gactcaacaa cctgctgatc gaacgtgccg
      721 cccggcgtac cggggtagcc gtccgttttc atcccatgcc ctgggcacgc tgcctcttca
      781 agacgtcggc cggtgagatg gacggggtgg tcggtgccag ctaccggccc gaacgcctgc
      841 gcattggcgt ctacccattg aaggatggtc agccccacac cgaacgccgc ctcatgcgca
      901 acagctactt gctctatcgc cgcaagggcg acccgcgcgt gcactgggat ggccagcgcc
      961 tgcaggtgaa cggcgtcgtg ggggtacaga ccggtttctc ggttgccggc acgctgcagc
     1021 gcctgggagc agcggttgac cagcagcacc gcctaccccg cccactgttg cagcaactgc
     1081 tgtcaggccg gcttcaggcc atcgcattga cggcggccga aggccagcgc ctgctggccg
     1141 aacatcctca aggcagcacc gtggaggccc tgccagcccc cctggaggag aaggatttct
     1201 atctcgtgtt ctcccgccag ttccagcggc aacacccggc tctggtcgag cggctctggc
     1261 aagcgctatg gctcgagcaa caaacaccgg agtggcgcac cgaatcaggg cgcttccgct
     1321 gacagacgta ccgcttttga cgatcccggc aataaccctc gccttctcag cctgaccatg
     1381 cggtcgtatg atcgaaagcc ttttgcccgc cacgagcggg caaaagccgg ctcacacaag
     1441 accaccagac cagaccggca ccagccggcg caatggagaa ttcgccatga ttggccagat
     1501 gatggacaga cccctgctga tttccagcct gctcgaattt gcggtggtga accacccgca
     1561 agccgagatc gtgacccgcc gcgtggaagg ggatatccac cgctacacct ggatggacgc
     1621 cgccgcccgc agcgcccgcc ttgccaatgc cctgaaccgt ctgggcgtga agactggcga
     1681 ccgcatcggt acgctggcct ggaacaccta ccgtcatctg gaactgtact ttggtgtgtc
     1741 cggcatgggc gccgtgctgc acaccatcaa cccgcgactg ttcccggagc agatcgtgtg
     1801 gattgccaat cacgccgaag accgcttcct ctttgtcgac ctgaccttcc tgccgctggt
     1861 ggatgccacc cgggcccacc tgaagtcgct cgaaaaagtg atcgtgctgg ccgacaaggc
     1921 cgccatgccc gccggccgcg atgacctggt ctgctacgaa gacctgcttg cccccgagtc
     1981 cgacagcttt gcctggccgg ccctcgatga gcgcactgcc gcctccatgt gctacacctc
     2041 gggcaccacc ggtaatccca agggcgtgct ctacacgcac cgctccaccg tactgcatgc
     2101 catgggttcg ctgcacagtg gcgccctcag catcggccgc gacaccgtgt tgctgcccgt
     2161 ggtaccgatg ttccatgtta atgcctgggg caccccgtac agcgccgcca tgtctggcgc
     2221 caaaattgtc ttccccggtg ccggcctcga cggcaagaac ctgcacgaac tgatcgacaa
     2281 ggaacaggcc accctgctgc tgggcgtgcc caccgtgtgg ctgggcctgc tcaactacct
     2341 cgacagcatc ggcgcgaaaa tgcacagcgt gaatcatgtc gtcgtaggcg gctcggccgc
     2401 gccgctggcc at
//
LOCUS       DASUEG010000589         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1144306, whole genome shotgun sequence.
ACCESSION   DASUEG010000589 DASUEG010000000
VERSION     DASUEG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144306"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /gene="narH"
                     /locus_tag="VFX11_11045"
     CDS             complement(<1..292)
                     /gene="narH"
                     /locus_tag="VFX11_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114168.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX4939205.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ESKPGVGYPKEWENQKKWKGGWERKSDGTLQPRIGARWRVLANIFANPDLPEI"
     gene            complement(308..>3293)
                     /locus_tag="VFX11_11050"
     CDS             complement(308..>3293)
                     /locus_tag="VFX11_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX4939206.1"
                     /translation="TPDAHFFTEVRYKGTKTVAITPDYSEVAKLTDIWLNPKQGTDAA
                     LGMAFGHVILKEFHLENPSEYFVDYCRRYTDMPFLVVLKQRVDGSYESGRFLRASDLQ
                     GNLEQVNNPEWKTIALDSNSGNLVAPNGSIGFRWGEQGEKGKWNLEAKEGGKDKDVSL
                     QLTLLGQQDSIASVAYPYFGGIENEHFTANKHQDILHRNIPAKKIALADGSTALVATV
                     YDLMLGNYGIDRGLGGAHVASSYDQNIPYTPAWQESITGVPRARVIQVAREFADNAHK
                     TKGRSMIIVGAAMNHWYHMDMNYRSLINMLVMCGCIGQTGGGWAHYVGQEKLRPQTGW
                     TPLAFALDWHRPPRHMNSTSFFYAHTDQWRYEKLHVSEILSPLADPEEWKGSLIDFNV
                     RSERMGWLPSAPQLARNPLQVSRAAAAAGVDPKQYVVDQLKSGALKFACEDPDAPENF
                     PRNMFIWRSNLLGSSGKGHEYFLKHLLGTKHGVQGKDLGAEGGEKPLEVKWHDQAPEG
                     KLDLLVTLDFRMSTTCLYSDIVLPTATWYEKNDLNTSDMHPFIHPLSAATDPAWESRT
                     DWDIYKGIAKKFSEVAKGVLGIEKDIVTIPTQHDTPGELAQALDVKEWKKGECDLIPN
                     RTAPNFVVVERDYPNTYKKFTSVGPLLNKLGNGGKGINWNTDDEVAFLKKLNYEVTEE
                     GVSQGCARIETDIDAAEMILTLAPETNGNVAVKAWDALGKITGRDHKHLAEPKHEEKI
                     RFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNVHELIPWRTLTGRQQFYQDHKWML
                     GFGEGFVSYRPPVDLKTVAPMLNHKPNGNKEVVCNWITPHQKWGIHSTYTDNLLMLTL
                     SRGGPIVWMSEGDAKRAGIEDNDWIEVFNVNGAIACRAVVSQRVPNGMVMMYHAQEKI
                     VNTPGSEITGTRGGIHNSVTRTVTKPTHMIGGYAQQSYGFNYYGTVGANRDEFVVVRK
                     MNKVDWLDGDAGDQLPQPLQTL"
BASE COUNT          580 a          975 c          942 g          796 t
ORIGIN      
        1 cgatctccgg cagatccggg ttggcgaaaa tattcgccag cacccgccac ctggcaccga
       61 tgcgcggttg cagagtgccg tccgacttgc gctcccagcc acctttccat tttttctggt
      121 tttcccattc cttgggataa cccacgccgg gtttggattc cacgttgttg aaccaggcgt
      181 attcgacgcc ttcccgggag gtccagacgt ttttgcaggt caccgaacag gtgtggcagc
      241 cgatgcactt gtccagattc aataccattc ctacttgtga acgaatcttc atttgcatgg
      301 cctccgacta cagggtttgc aacggttgtg gaagttgatc gccggcgtcg ccatcgagcc
      361 agtccacctt gttcatcttt cttaccacca cgaattcgtc gcggttcgcg cctacggttc
      421 cgtaataatt gaaaccgtag ctttgctgcg cgtagccacc gatcatgtgg gtgggtttgg
      481 tgacagtgcg ggtcaccgag ttgtgaatac cgccccgggt gccggtgatt tccgatccgg
      541 gtgtgttcac gattttttcc tgggcgtggt acatcatcac catgccgttg ggcacgcgct
      601 ggctcaccac cgcgcggcag gcaatggcac cgttgacatt gaagacctcg atccagtcgt
      661 tgtcttctat acccgctctc tttgcatcac cttcgctcat ccacacaatg ggaccgccgc
      721 gcgacaatgt cagcatcagc agattgtcgg tgtaggtgga gtgaatgccc cacttctggt
      781 gcggcgtgat ccagttgcac accacttcct tgttgccgtt gggtttgtgg ttcagcatcg
      841 gtgccaccgt tttcaggtcc actggcgggc gatagctgac aaagccctca ccgaagccga
      901 gcatccactt gtgatcctgg tagaactgct gccgcccggt gagggtgcgc cagggaatca
      961 gctcatgcac gttggtgtaa ccggcgttgt agctgacgtg ttcgtcttcc aggccgctcc
     1021 aggtgggcga cgagatgatc ttgcgcggct gcgcctggat atcgcggaag cggattttct
     1081 cctcgtgctt gggttccgcc agatgcttgt gatcgcgacc ggtgattttg cccagcgcat
     1141 cccaggcttt taccgcgacg ttgccgttgg tttccggcgc cagcgtcagg atcatttccg
     1201 ctgcatcgat atcggtttcg atgcgggcac acccctggct cacgccttcc tcggtcactt
     1261 cgtagttgag cttcttcagg aacgccactt cgtcgtcggt attccagttg atgcccttgc
     1321 cgccgttgcc gagcttgttc aacaacgggc ccaccgatgt gaatttttta taggtgttgg
     1381 gataatcccg ttccaccacc acgaaattgg gcgcggtcct gttggggatc agatcgcatt
     1441 cacctttttt ccactccttc acgtccagcg cctgcgccag ttcccccggc gtgtcgtgct
     1501 gggtgggaat ggtgacgata tccttttcaa tgcccagcac acccttggcc acttccgaga
     1561 attttttcgc gatgcccttg tagatatccc agtcggtacg ggattcccac gccggatcgg
     1621 tggcagcaga cagcgggtga atgaaggggt gcatgtccga tgtgttcagg tcgttctttt
     1681 cataccaggt ggcggtgggc agcacgatgt cggaatacag gcaggtggtg gacatgcgga
     1741 aatcaagcgt caccagcaga tccagtttgc cttccggcgc ctggtcgtgc catttcactt
     1801 ccagcggttt ctcaccgcct tcagcgccca gatccttgcc ctgcacaccg tgcttggtgc
     1861 ccagcagatg cttgaggaaa tactcatggc ccttgccgct ggagcccagc aggttggagc
     1921 gccagatgaa catgttgcgc gggaagtttt ccggcgcatc cgggtcttca caggcaaatt
     1981 tcagcgcgcc ggatttcagt tgatccacca catactgttt tggatcgaca cctgccgccg
     2041 ctgccgcacg gctgacctga agaggattcc gggccagttg cggcgcgctg ggcagccagc
     2101 ccatgcgttc cgagcgcacg ttgaaatcga tcaggctgcc tttccattcc tcaggatcgg
     2161 ccagcggcga cagaatctcg ctgacatgca gtttttcgta ccgccactga tcggtgtgcg
     2221 catagaaaaa cgaggtgctg ttcatgtggc ggggcggacg gtgccagtcc agcgcgaatg
     2281 ccagtggcgt ccagccggtt tgcggccgca gtttttcctg tccgacatag tgagcccagc
     2341 caccaccggt ctgaccaatg caaccgcaca tgaccagcat gttgatcagc gagcggtagt
     2401 tcatgtccat gtggtaccag tggttcatcg ccgcgccgac gatgatcatc gagcgccctt
     2461 tggtcttgtg ggcgttgtcg gcgaattcac gcgccacctg gatcacgcgg gcacggggta
     2521 cgccggtgat ggattcctgc cacgccgggg tgtagggaat gttctggtcg taggacgacg
     2581 ccacatgggc accccccagg ccacgatcga tgccgtaatt gcccagcatc aggtcgtaca
     2641 cggtggccac cagtgcggtg ctgccatccg ccagcgcgat ctttttcgcc ggaatattgc
     2701 ggtgcaggat gtcctggtgt ttgttggcag tgaagtgttc gttctcgatg cccccaaagt
     2761 acggatacgc aaccgaggcg atggaatcct gttgtccgag caaggtcagt tgcagtgaca
     2821 cgtctttgtc cttgccacct tccttcgctt ccagattcca cttgcctttc tcgccctgct
     2881 cgccccagcg gaaaccgatg gaaccgttcg gcgccaccag attgccactg ttgctgtcga
     2941 gagcgatggt tttccattcc ggattattga cctgttccag attgccctgc agatcgctgg
     3001 cacgcaggaa acggccgctt tcataactgc catcgacgcg ctgcttcagc accaccagga
     3061 acggcatatc ggtgtagcgg cggcaataat ccacgaagta ttcactggga ttttccaggt
     3121 ggaattcctt caggatcaca tggccgaacg ccatgcccag cgccgcgtcg gtgccctgct
     3181 tcggattcag ccagatgtcg gtgagcttgg cgacttcgga ataatccggc gtgatggcaa
     3241 cggtcttggt gcccttgtag cgcacctcgg taaagaagtg ggcatccggc gtg
//
LOCUS       DASUEG010000590         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1174123, whole genome shotgun sequence.
ACCESSION   DASUEG010000590 DASUEG010000000
VERSION     DASUEG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174123"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1197)
                     /locus_tag="VFX11_11055"
                     /pseudo
     CDS             complement(1..1197)
                     /locus_tag="VFX11_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518935.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(1208..>2731)
                     /locus_tag="VFX11_11060"
     CDS             complement(1208..>2731)
                     /locus_tag="VFX11_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4939207.1"
                     /translation="KSIAKNVAKSMSVAVLAAMLLTACGQAHDEHAGEHGHETEAHGH
                     DAGPEIPRGPHRGKLFEKDGFGLEVTIFEDGVEPEFRVYPTLNDKPVDPAGIQLVVTL
                     ERLGATQTIRFSPKDDYLLGDQVVYEPHSFSVKLAASYQQKSYAFQYDQIEWRVDLSP
                     EQATAAGLEILKAGPAELADEVELQGEIRVAPNSETVVLTPVAGVVVSAPVSLGQQVK
                     AGDVLATLESRELADLKRAYLEARERSRLAESTFQREAMLWKEKITAEQDYLAAKSAK
                     AEADINVASSRAALLSFGLTEGELKSLSLEQAGNLARLVLRAPRNGRVTARDLAPGQR
                     VSPESVLFTIADLDRLVAVLSASPAQLDGLKPGQMVRVSQVGGTPVSAAVTGNGRVQV
                     VSPQLDEGNRAASVFVAMDNAALWRPGQFVKAKVTRATAPAAVTVSAEALQSFRDWTV
                     VFARYGNQFEVRPVTVGRRSSHVVEILDGLPAGQEYVGKNSFVLKADIGKSEAAHDH"
BASE COUNT          531 a          930 c          729 g          541 t
ORIGIN      
        1 cgcccagact catcaggttg gcactgacct tgtgggtcac catgcccgtg atcgtcatca
       61 gcatggccag cggaatcacc gccgccgtaa tgaatgccgc gcgaaagttc ccaaggaacg
      121 caaacagcac agtaatcacg agcagcgcac cttccagcag gtttttctgc acggtggcaa
      181 tggtggcatc caccagttcg gtgcggtcat acacgagtac cgcacgtatg ccctccggca
      241 gacttttctg cacctccacc agcttctgtt ttacgcgctc ggccactgtc cggctgttct
      301 cgccgatcag catgaaggcg gtgcccaaca ccacctcgcg gccgttctcg gtcgccgcac
      361 cggtacgcag ttcctgtcca aagccgatat cagccacgtc gcgcagatac acgggcacgc
      421 tgtcctcggt cttgatgaca atggcacgca catcctccag cgactgcacc tgccccggcg
      481 cgcggatcag gtactgctcg ccacgacgct cgatataacc ggcaccgaca ttggcgttgt
      541 ttcgttccag cgccgtcatc acgtcggcaa gggacagtcc gtaagacgcc aattttcagg
      601 atacggcgcc accagatact gtttctggaa gccgccgatg ctgttgattt ccgtcacgcc
      661 gggcaacgtg cgcagttgcg gctttaccac ccagtcctgc aaggtacgca ggtcggtcgc
      721 atcgtaggca gaaccatccg gcttgcgtgc gtcaggatca gcttccaccg tccactggct
      781 gatttccccc aagccggtgg agatcggccc cagtgtcggc tccagtccgc cgggtagctg
      841 gccccgtgcc gcctgcaggc gttcgctcac cagttggcgg gcaaagtaga tatccgtacc
      901 ttcttcaaac accaccgtga tctgcgacaa gccgtaacgc gacagcgagc ggctttcctt
      961 caggcgcggc aacccgccca gtactgcctc cagcggcagc gtgacacgct gttcggcctc
     1021 cagtggtgaa taacccggtg cttcggtgtt gatctgcacc tgcacattgg tgatgtccgg
     1081 cacggcatca atcggcaaac ggaaatagtt gtagataccc agcgccgcca cggcgagcgt
     1141 gacgaccagc accagccagc gctgctgcag ggcaaagcgc aaaagacgat caaacatggc
     1201 catgccctca gtgatcgtgt gctgcttcgc tcttgccgat gtcggccttg agcacaaagc
     1261 tgttcttgcc cacgtactcc tgtcctgccg gcaagccatc aaggatttcg acaacgtggc
     1321 tgctgcgacg ccccacggtg accggacgca cttcgaactg gttgccgtac cgggcaaaca
     1381 ccacggtcca gtcgcggaag gactgcaatg cctccgcact gaccgtcacg gcagccggag
     1441 ctgtcgcccg tgtcactttc gccttcacga actgacccgg tcgccacagg gcggcattgt
     1501 ccatggccac aaaaacggat gccgcacgat tgccttcatc cagttgcggg ctgacaacct
     1561 gtacacgccc gttgcctgtt acagcagcgc tgaccggagt gccgccaacc tgactgactc
     1621 ggaccatctg gcccggcttc aatccgtcca gctgtgccgg tgacgccgac agcacagcca
     1681 cgagacgatc cagatccgca atcgtgaaca acaccgactc cggcgacacc cgctgtcccg
     1741 gcgccagatc acgcgccgtc acccgcccgt tgcgcggcgc acgcaacacc agccgggcca
     1801 gattgccggc ctgctcaaga ctgagggatt tcagttcgcc ttccgtcaga ccaaacgaca
     1861 gcaatgcggc gcgactgctg gccacattga tgtcggcctc tgccttggca cttttcgctg
     1921 ccaggtaatc ctgctcagcc gtgatttttt ctttccagag catggcctcg cgctggaaag
     1981 tcgattcggc caaacgagac cgctcgcgcg cctccagata cgcacgcttg agatcggcca
     2041 gttcacggct ttccagtgtg gccagcacat cccctgcttt cacctgttgc cccaggctga
     2101 ccggcgccga taccaccaca cctgccaccg gtgtcagcac caccgtttca ctgttcggtg
     2161 ccacacggat ttcgccctgc agttccactt catcggccaa ctcggccgga ccggccttca
     2221 ggatttccag gccagcggcc gtcgcttgct ccggtgacaa gtcgacacgc cattcaatct
     2281 ggtcatactg gaaggcgtag gatttctgct ggtagcttgc cgccagcttc accgagaaag
     2341 aatgtggctc gtacaccacc tgatccccca gcagatagtc atccttgggt gaaaaacgaa
     2401 tggtctgcgt ggcgcccagg cgctcaagtg tcacgaccag ctgaatgccg gccggatcaa
     2461 ctggtttgtc attgagcgtg ggatagaccc gaaactcggg ctctacccca tcctcgaaaa
     2521 tcgtgacctc cagaccaaaa ccgtctttct cgaacagctt gccgcgatgc gggccgcgcg
     2581 gaatctccgg tccggcatcg tgaccgtgcg cctctgtttc atgtccatgt tcgccagcat
     2641 gttcatcatg tgcctgcccg cacgccgtca acagcatggc cgccaacaca gccactgaca
     2701 tgctttttgc aacgttcttt gcaatgctct t
//
LOCUS       DASUEG010000591         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_357227, whole genome shotgun sequence.
ACCESSION   DASUEG010000591 DASUEG010000000
VERSION     DASUEG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357227"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..880)
                     /locus_tag="VFX11_11065"
     CDS             complement(53..880)
                     /locus_tag="VFX11_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4939208.1"
                     /translation="MSQRRLDLPLVIEPAQLEPLLGTPNLLVVDLCRPEQYATAHIPG
                     AVFLPFTQLSRGVPPGPGKLPAENLMSRVMSSIGLTPDTHVVAYDDEGGGWAGRLIWT
                     LDVIGHPHYSYLNGGLHAWIAERRKLEGGIAMPAPSQYSAHYTNTGITTKEDILAHLK
                     DPNFVVWDARSRDEYFGNRVSAQKNGHIPGAVNYEWTRAMDPHNHLRIRDPEKLRQEL
                     ASLGITTDKTIVTHCQTHHRSGFTYLVGKVLGFPRISAYPGSWSEWGNDPTTPVETH"
     gene            complement(995..>2719)
                     /locus_tag="VFX11_11070"
     CDS             complement(995..>2719)
                     /locus_tag="VFX11_11070"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4939209.1"
                     /translation="DEKLLPVLPAPRQRVDALLKKREIPLQEVARTIAADPVMSLHLL
                     RECQRLFGRRLGGVPGNVQHCVSLLGLDKLRALLGTLQSLRGDPADPAIHPYLRAIGG
                     SVHALAQVGAWTRYRSQPGADHLLQAALLYSAAEWSLWYFAGTDMQIVDRLIRQEQIP
                     PKQAELAVFGCSRDQIAHGLGQRWKFPADVLECLDSPQLPDLGFMLRCARAAQQDPNY
                     RLPNRDHHGHMIRSAGMTLHLGDWLARELALDWYSPGTRHCLEIAAVYLNIPFDELRV
                     LIRDCALAISHDWTVPGVVAPAANLIWPVQPRRPRRLKLRQLPAAVAKLRASAAKTPT
                     PVPVQPVPQAPPLASKAPTPSPRPAPIGIANDHLPPELDRKRILNAPLPPVAPADTSH
                     RHAGFRSAAHKQTFERFLRELPEPKHAGTDQDALRAVADQLLSCTELNRVVAILYQRQ
                     SDQAESLYAAGCDNQSGLQRLKVKLQPVNLFTHLLKQPASVWISPDRPNPMNALVPGS
                     LKQASQSSEFFVMSVFDHRGPFGLFYADRGTGGQTALSNAEYAAFKAACTACSKRLVT
                     RGKQGSSR"
BASE COUNT          422 a          776 c         1013 g          508 t
ORIGIN      
        1 gggttctctc gttttcaaaa cagggaatgc agcaggttgg acccgcttgt gatcagtggg
       61 tttccaccgg tgtcgtcggg tcgttgcccc actcggacca ggagccgggg taggcgctga
      121 tgcgggggaa gcccagcacc ttgcccacca ggtacgtgaa gccggaacgg tggtgggtct
      181 ggcaatgggt gacgatggtc ttgtcggtgg tgatgcccag tgatgccagt tcctgccgca
      241 gtttttccgg atcgcggatg cgcaggtggt tgtgcgggtc catggcgcgg gtccattcgt
      301 aattgacggc gccgggaatg tggccgttct tctgcgcgct gacccggttg ccgaagtatt
      361 cgtcgcggga gcgggcgtcc cagaccacga agttggggtc tttcagatgg gccaggatgt
      421 cttccttggt ggtgatgccg gtgttggtgt agtgcgcgct gtactggctg ggggcgggca
      481 tggcgatgcc gccttccagt ttgcgccgct cggcgatcca ggcatgcagg ccgccgttca
      541 ggtaggaata gtgggggtga ccgatcacgt cgagggtcca gatcaggcgt ccggcccagc
      601 cgccgccttc gtcgtcgtag gcgaccacgt gggtgtcggg ggtgaggccg atggaggaca
      661 tgacccggga catgaggttt tccgccggca gtttgccggg cccgggcgga acgccccgcg
      721 acagctgggt gaagggcaga aacacggcgc ccgggatgtg ggcggtggcg tactgctcgg
      781 ggcggcacag gtctaccacc agcaggttcg gcgtgcccag caggggttcc agctgggccg
      841 gttcgatcac cagcggcaga tccagacggc gttggctcat tcggtttcct tcagaatacg
      901 ggcaaggtcc ccggggatgg gccgcaacag ggacaatatt gccaccagtt tacttgaacc
      961 ccgtgtctgc gctcaaagtt tttagtacgg gtgctcagcg gctgctgccc tgtttgcccc
     1021 gggtgacgag ccgcttgctg caggcggtgc aggcggcctt gaaggcggcg tattccgcgt
     1081 tggacagcgc ggtctggccg ccggtgcccc gatcagcgta gaaaagtccg aaggggcccc
     1141 ggtgatcgaa gacggacatg acgaagaact cgctggactg gctggcctgc ttcagcgatc
     1201 cgggtaccag ggcgttcatg gggttggggc ggtccgggct gatccagaca ctggcgggct
     1261 gtttcagcag atgggtgaac aggttcaccg gctgcagttt gactttcagg cgttgcaggc
     1321 cggattggtt gtcgcagccg gcggcataga ggctctcggc ctgatcgctc tggcgttgat
     1381 agaggatggc caccacgcgg ttcagttcag tgcaggagag cagctgatcg gccacggcgc
     1441 gcagtgcgtc ctggtcggtg ccggcgtgtt tcggttccgg cagttcgcgc aggaagcgtt
     1501 cgaaggtctg tttgtgtgcg gcggaacgaa agccggcgtg gcggtgcgag gtgtcggctg
     1561 gcgccaccgg cggcagcggg gcattgagga tgcgcttgcg gtccagttcc ggtggcaggt
     1621 gatcgttggc aatgccgatg ggcgccgggc gcggggatgg cgtcggcgcc ttgctggcaa
     1681 gggggggtgc ctgcggcacc ggctggaccg gcactggtgt gggtgtcttt gcagctgatg
     1741 cgcgcagttt ggcgacggcg gcaggcaact ggcgcagttt caggcgtcgg ggccgccgcg
     1801 gctgcaccgg ccagatcagg ttggcggcgg gggctaccac gccagggacc gtccagtcgt
     1861 gggaaatggc cagggcgcag tcgcggatca ggacacgcag ttcatcgaag ggaatattca
     1921 ggtacaccgc cgcaatttcc aggcagtggc gggtacccgg ggagtaccag tccagcgcca
     1981 gttcgcgcgc cagccagtca cccaggtgca gggtcatgcc cgccgaccgg atcatgtggc
     2041 cgtggtggtc gcggttgggc aggcggtagt tcgggtcctg ttgcgcggcc cgggcgcagc
     2101 gcagcatgaa acccaggtcg ggcagctgcg gactgtccag gcattccagt acatccgccg
     2161 ggaatttcca gcgctggccc aggccatggg caatctggtc gcggctgcag ccgaacacgg
     2221 ccagttcggc ctgtttgggg ggaatctgtt cctggcggat caggcggtcg acgatctgca
     2281 tgtcggtgcc ggcaaaatac cagagagacc attccgcggc gctgtacagc agggctgcct
     2341 gcagcaggtg gtcggcgccg ggctggctgc ggtagcgggt ccaggcgccg acctgcgcga
     2401 gggcatgcac gctgccgcca atggcgcgca ggtaagggtg aatagccggg tctgccggat
     2461 cgccccgcag tgattgcagg gtgcccagca gtgcgcgcaa cttgtccagc cccagcagcg
     2521 atacgcagtg ctgcacattg cccggcacac cacccaggcg gcggccgaac agtcgctgac
     2581 attcccgcag caggtgcagg ctcatgaccg gatcagcggc aatggttcgt gccacttcct
     2641 gcaggggtat ttcccgtttc ttcagtaacg cgtccacccg ctgccgtggc gcaggcagca
     2701 ccggcagaag cttctcatc
//
LOCUS       DASUEG010000592         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1438692, whole genome shotgun sequence.
ACCESSION   DASUEG010000592 DASUEG010000000
VERSION     DASUEG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438692"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX11_11075"
     CDS             <1..279
                     /locus_tag="VFX11_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939210.1"
                     /translation="AIGFYALRRQARDRYDTAIKEAIERFKAEVGPEIEQRVKQGMLE
                     GVKSLPSREVLRETTRTIAKTGLDLMGDSLKMATRSRPRKPGTPLDDD"
     gene            complement(294..1061)
                     /locus_tag="VFX11_11080"
     CDS             complement(294..1061)
                     /locus_tag="VFX11_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX4939211.1"
                     /translation="MPQTPEESMIRYLALEVIEAAINLALSHEPGALERIREHKGRVL
                     RIKTTGPDWMFFVALCDDGVQLFAEYEEVVDARLTLPFMLLAQYVLGTGTDEVQGAEG
                     VRVSGDLLMLAELMQIVQEFSIWTLSKRIINNWLPQYEGFSGLLEALKNHDPAWIVRL
                     EHLPQMANETLLAVRAQSELQQRQMSELQAIRRQLDADRRANQVSTIIGFCLILVAFL
                     AHNGYLQVPAIENISLDTLILLILSVVLLVPRMLGRR"
     gene            1299..2483
                     /locus_tag="VFX11_11085"
     CDS             1299..2483
                     /locus_tag="VFX11_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939212.1"
                     /translation="MSIPLVAGIIGWGTNWVAIQMTFYPINFFGIPPWLGWQGIIPKR
                     GRKMAGIVVENTLDKISTMQEIFDEFEPHKIAHHIIKVADSRIEELTDDIMNERNAVL
                     WENLPTVLKNRAYARARRQLPQMLDSLIEDMQANIEDLVDPKDLIIKKLSADKHLLNR
                     VFLECGEAEFKFVINSGFWFGLLFGIPQMIWWWFDKSWWTLPACGLFVGLATNWLALN
                     LIFRPLNPVKVGPITLQGLFLRRQNAVSEVFCRLVTQDILTVGHIMTEIFRGPRSDRA
                     KSMLKRHLRPIIDGGVVKTVAQLAVGPEGFVGLKRAIEEKTIEMSLASFDDPVFTRER
                     GKVVERMFRDRMQAMSAEDFQALLRPAFEEDEWILIALGGVLGFVAGMAQLVFMFSDV
                     TF"
BASE COUNT          601 a          845 c          793 g          556 t
ORIGIN      
        1 gccatcggct tctatgccct gcgccgccag gccagggacc gctacgacac cgcgattaaa
       61 gaagccatcg aacggttcaa ggcagaagtg gggcccgaga tcgaacagcg ggtaaagcag
      121 ggcatgctgg aaggagtgaa atcgttacca tcccgcgaag tgctgcggga aaccacgcgc
      181 acgatcgcca aaaccggact ggacctgatg ggtgacagcc tgaaaatggc cacccgcagc
      241 cgcccccgca agccgggcac gccgctcgac gacgattgac cgccagcaag acctcagcgc
      301 cgccccagca tccggggcac cagcaacacc accgacagaa tcagcagaat cagggtgtcc
      361 agcgagatat tttcgatggc aggcacctgc aggtacccgt tgtgggccag gaatgccacc
      421 aggatgagac agaaaccgat gatggtgctg acctggttgg cacggcggtc ggcatccagt
      481 tgccggcgga ttgcctgaag ttcgctcatc tgccgctgct gcagctccga ctgggcccgc
      541 accgccaaca gggtttcatt ggccatctgg ggcagatgtt ccagccgcac aatccaggcg
      601 ggatcatgat ttttcaacgc ttcgagcaga ccgctgaaac cttcatactg cggcagccag
      661 ttattgatga tgcgcttgct cagggtccag atgctgaact cctgcacgat ctgcatcagt
      721 tccgccagca tcagcagatc accactgacc cgcacgccct ccgcgccctg cacttcgtcc
      781 gtgccagtac ccagcacgta ctgcgccagc agcatgaaag gcagggtcag gcgggcatcc
      841 accacttcct catactccgc aaacagctgc acgccgtcgt cgcacagggc cacgaagaac
      901 atccagtccg ggccagtggt cttgatccgc agcacccgcc ccttgtgttc ccggatccgc
      961 tccagcgccc cgggttcgtg ggacaatgcc agattgatgg ccgcctcgat cacttcaagg
     1021 gccaggtagc gaatcatgga ttcttcgggc gtttgcggca tggggaaagg cccccttcgt
     1081 tatagttctg ggcatcccgg cgggatgccg aaatttacca ccggcctgcc caaaaatccc
     1141 gggatttttt ttttgtgcac gttataatac cccggctttt tgggcctata cgtcttgtat
     1201 tcgacgacat ttgtggtctt tctgtaacct tcagggaaca tcactcgatg cttgagtttt
     1261 acaacgagtt tgtaaaccac ccggacttct gggcctatat gagcatcccc ctcgtggccg
     1321 gcatcatcgg ttggggcacc aactgggtgg ccatccagat gacgttttac cccatcaatt
     1381 tcttcggcat tccgccatgg ctgggctggc agggcatcat ccccaaacgc ggccgcaaaa
     1441 tggcgggcat cgtggtggaa aacacgctcg acaagatcag caccatgcag gagatcttcg
     1501 acgagttcga gccacacaaa attgcccatc acatcatcaa ggtcgctgat tcccgcatcg
     1561 aggaactgac cgacgacatc atgaatgagc gcaatgcggt gctgtgggag aacctgccca
     1621 ctgtactgaa aaaccgcgcc tacgcccgtg cccgccggca gctgccgcaa atgctcgaca
     1681 gcctgatcga ggacatgcag gccaatatcg aagatctggt ggatcccaag gatctgatca
     1741 tcaagaaact gtcggccgac aagcacctgc tcaaccgcgt ctttctggaa tgcggcgaag
     1801 ccgagttcaa gttcgtcatc aattccggat tctggttcgg cctgctcttc ggcatacccc
     1861 agatgatctg gtggtggttc gacaagagct ggtggacatt gcccgcgtgc ggtctcttcg
     1921 tcgggcttgc caccaactgg ctggcgctga acctgatctt tcgccccctg aaccccgtga
     1981 aagttggccc gattacactg cagggcctgt tcctgcggcg ccagaatgca gtatccgaag
     2041 tgttctgccg cctggtgacg caggacatcc tcacggttgg ccacatcatg acggaaattt
     2101 tccgcggccc ccgctccgac cgcgccaagt ccatgctgaa acggcacctg cgcccgatca
     2161 tcgacggcgg cgtcgtgaag accgtggcac agctggcggt cggtcccgag gggtttgtcg
     2221 gtctgaagcg cgccatcgag gaaaagacca tcgaaatgtc gctggcgtcc ttcgacgatc
     2281 cggtgttcac ccgtgaacgg ggcaaggtgg tggagcgcat gttccgcgac cgcatgcagg
     2341 ccatgtcggc ggaggatttt caggcactgt tgcgaccggc gttcgaggag gacgaatgga
     2401 tactgattgc gctggggggg gtactggggt tcgtggccgg catggcccag ctggtgttca
     2461 tgttttccga tgtgacattt tagtgcctat gattctgaag ccttgatgtg gcgcaaaaaa
     2521 agccaaagcc agtctgtggc catccggcgt agaatgcagc acggcccgcc aggcgccatc
     2581 atggcaacgc tgtgatctgc actgatcagc cgcttctgat agaacaataa aatactgaac
     2641 catactgaat cttgagtggc cttcccatcg ttacgcaaac caatacacgc cagcagaatg
     2701 gcccaaaaca ggccggaacc aggagagtct caatgatacg agggggcgtt gagtcgacat
     2761 gatccatgcg ttttccccgg atcacctgct tggcg
//
LOCUS       DASUEG010000593         5716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1932419, whole genome shotgun sequence.
ACCESSION   DASUEG010000593 DASUEG010000000
VERSION     DASUEG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932419"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFX11_11090"
     CDS             <1..390
                     /locus_tag="VFX11_11090"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262865.1"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX4939213.1"
                     /translation="CDSDGRIDKYVDSGDLATSLPMPAYGDDEEIELAIFMVGAYQEI
                     LGDMHNLFGDTDAIDVTLDGNGGFKIVNMLKGDTVDHILRYVEYDPMQLQQQIHEQLL
                     MTGLSKELQSEFLGELREGLVGYTYLE"
     gene            complement(394..2442)
                     /locus_tag="VFX11_11095"
     CDS             complement(394..2442)
                     /locus_tag="VFX11_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4939214.1"
                     /translation="MPVTRHSFRFATLAALLAGIALACPVQVSATQQDLFELSLEELG
                     NIAITGPTLTKKKLSNVPASVTVFTRDEIRQLPVSTIEQLMNYVPGFQSARSSDNSMG
                     QPYSVRGRKFGVSGREVLVMINGMRIENFFTGGGANTYPAIPLANIARVEFIRGPGSA
                     LYGSNALTGMINIITDEAHNHLGANAGDPEQATVNWLQQLDTASIDGSLFLQGYRHNG
                     EDYQVQDTFSPNIISTHDPQRSSDALLQLTFNRHTRLQLLAAERDINDFYVTGFLSDG
                     FSHYDTRYYHGLLEHTLAWSDAITSSFQAGYSHFSMPVNSQATPAGALAAISAPSSTD
                     PLQFRSEVVTNETWMRWLNDWKLDDSKSLQFGLSYRNPGIDSAPINSNFDLQALHRQE
                     FPVTYREDFGNRSTVAETAGMDIAAAFAQYQTKLGEQADLVLGARYDSYSQIGSHVSP
                     RIALTLHPSTSDSFKLLYGRAFDAPMGAELYSINNPILEGNPDLQPETVDTWEIIWLR
                     QWQMGTFTANYFYNRFHDSIAQVVVDGVRTYDNISHENSDGMEFELASQLHANFSLRA
                     SVTHLFSQPDSSFRESDQLFALIGRFQQSFGYATLTLNHQSDKQTLSNNGATRETLDG
                     FWLANLKLGYYAGRNLEGYVEVMNLGDVDYTTPSMSGNIADGIPNRRRELRLGVAWEY
                     "
     gene            complement(2494..3045)
                     /locus_tag="VFX11_11100"
     CDS             complement(2494..3045)
                     /locus_tag="VFX11_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEX4939215.1"
                     /translation="MTTPSIDDDLPLVGISSCLTGQRVRYDGLDKFNLLVAERIAPYC
                     RLLPFCPEAVAGMGIPRPPINLVRTADGIRALGRDAPHRDVTDRLLTVADIFANSYPT
                     LCGYILQSRSPSCGLNTTPVLDLTQQQVLDSQGSGLFAQRIRARFPDLPLLNDTDLDD
                     ASIAHFLREIRLRAAAFGLRFKQ"
     gene            3044..3469
                     /locus_tag="VFX11_11105"
     CDS             3044..3469
                     /locus_tag="VFX11_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX4939216.1"
                     /translation="MAWFQQSIQLQPRRRGFHLVTREILPQIERELRTLRIGLAHFFL
                     QHTSASLTINENADPDVRVDFESYFNRLAPENAPYFTHTLEGPDDMPAHLKSSLLGCS
                     LTLPIQGGALALGTWQGIYLCEHRNEGGSRRLLVTLQGE"
     gene            3528..4667
                     /locus_tag="VFX11_11110"
     CDS             3528..4667
                     /locus_tag="VFX11_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX4939217.1"
                     /translation="MSAMRLVVLTMALLTTSWLAGPVLAQPESEPAVATEPSLGDVSL
                     FDPQDGYLDASAWLATRFGFLPVPVIVTEPAVGYGLGLAGIWFHEMPVKKPSGQTYFP
                     SLSGAVYLETENETRMAGGFHMQSWKDDDWRYTGFLARADINLESQTDLERPLQYNLN
                     GDFLLQRISLRLDDFWRLGAHYLYAGGDVQFEREELTLDTVSSALGLQLYYDGLDNPF
                     SPSSGAMLQFEPEYFAEQLGSDVSFAKWTLDAQYYLPLGRHRLAGRLVGGWADQKAPF
                     YNQPFIMLRGVPKMGYLGTEVAAIEVEWAYNLHPRWQLLAFAGAGHSSSERGLRVAES
                     ASVEAGGLGFRYLLASRLGLRVGADLTANSDDDYNLTLVVGTAWF"
     gene            4930..5202
                     /locus_tag="VFX11_11115"
     CDS             4930..5202
                     /locus_tag="VFX11_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939218.1"
                     /translation="MSDYDEIDETAVEPVAGEEASALPPAEEPAQITFDDGRDRTVSS
                     REAERRRLEDDMKRFLSGGGKITQVDPNVRMDPPRKPESNYGSRPI"
     gene            complement(5331..>5716)
                     /locus_tag="VFX11_11120"
     CDS             complement(5331..>5716)
                     /locus_tag="VFX11_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX4939219.1"
                     /translation="RVGNTCAGQFLSGTQDPLTLYPFRRMSRYSTVPQLLDLPEVQRA
                     IALNTLGQRVPMAPLYIYQGKLDELIPIRDVDAVVEHYCESGVPVQYRRVLNDHLLLA
                     ITGHRGALRYLSDRIAGIPAPSNCD"
BASE COUNT         1137 a         1571 c         1816 g         1192 t
ORIGIN      
        1 tgcgattccg atggccgcat cgacaagtat gtggattccg gtgatctggc caccagcctg
       61 ccgatgccgg cctatggcga cgacgaggaa attgagctgg cgattttcat ggtgggcgcg
      121 taccaggaaa tccttggcga catgcacaac ctgtttggcg acaccgacgc catcgacgtc
      181 acgctggacg gcaatggcgg tttcaaaatc gtcaacatgc tgaagggcga cacggtggat
      241 catattctgc gttatgtgga atacgatccc atgcagttgc agcagcagat ccacgagcag
      301 ctgctgatga ctggcctgag caaggaactg cagagcgaat ttctgggtga attgcgcgaa
      361 gggttggtgg gatacaccta cctggaataa cgctcagtac tcccacgcga cacccaaccg
      421 cagctcccgc ctgcggttgg gaatgccatc ggcgatattg ccggacatgg acggggtggt
      481 gtaatccaca tcacccagat tcatcacttc cacatagcct tccagattgc gccccgcgta
      541 ataccccagc ttcagattgg cgagccagaa tccgtccagc gtttcgcggg tggcgccgtt
      601 gttgctcagg gtctgcttgt cgctctggtg gttcagggtc agggtggcgt aaccaaagga
      661 ttgctggaag cggccgatca gcgcgaacag ctggtcggat tcgcgaaagc tggaatccgg
      721 ctggctgaac agatgcgtaa cgctggcgcg caagctgaaa ttcgcgtgca gctggctggc
      781 gagttcgaat tccatgccgt cgctgttttc gtgggaaatg ttgtcatagg tgcgcacgcc
      841 gtccaccacc acctgtgcaa ttgaatcgtg aaaccggttg tagaaatagt tggcggtgaa
      901 ggtgcccatc tgccattgcc gcagccagat gatttcccag gtgtccactg tctccggctg
      961 caggtcggga ttgccttcca ggatggggtt gttgatgctg tacagctcgg cacccatcgg
     1021 cgcgtcgaag gcgcggccat atagcagttt gaagctgtcg ctggtggaag gatgcagtgt
     1081 cagcgcgatg cgtggactga catgcgagcc aatctgggaa taggaatcgt agcgcgcgcc
     1141 cagcaccaga tccgcctgtt cgcccagctt tgtctggtat tgcgcaaacg cagcagcgat
     1201 gtccatcccg gcggtttctg caacggtgct gcgattgcca aaatcctccc ggtaggtaac
     1261 aggaaattcc tgccgatgta gcgcctgcag atcgaaattg ctgttgatcg gcgcgctgtc
     1321 gatgccaggg ttgcgatagc tcaggccgaa ctgcaggctc ttgctgtcat ccagtttcca
     1381 gtcgttgagc cagcgcatcc aggtttcgtt ggtgacgact tccgagcgga attgcagtgg
     1441 atcggtgctg gacggtgcgc tgatggccgc cagcgcgcct gccggcgtcg cctgggaatt
     1501 gaccggcata ctgaagtgcg aatagccagc ctggaagctg ctggtgatgg catccgacca
     1561 ggccagggta tgctccagca ggccgtggta gtagcgtgtg tcgtagtggc tgaagccgtc
     1621 ggacagaaaa ccggtgacgt agaaatcgtt gatatcgcgt tccgctgcca gcaattgcag
     1681 ccgggtgtga cggttgaagg tcagctgcag taacgcgtcg ctgcttcgtt gcggatcatg
     1741 ggtggaaatg atgttgggac tgaaagtgtc ctgcacctgg tagtcttcgc cgttgtggcg
     1801 gtagccctgc agaaacaggc tgccgtcgat gctggcggtg tcgagctgct gcagccagtt
     1861 cacggtggcc tgttccggat cgcctgcatt ggcgcccagg tggttgtggg cttcgtccgt
     1921 gatgatgttg atcatgccgg taagagcgtt ggacccgtaa agggctgagc ctgggccgcg
     1981 aatgaattcc acccgggcaa tgttggccag cggaatggcg gggtaggtat tggcgccgcc
     2041 gccggtgaaa aaattctcga tccgcatgcc gttgatcatc accagcactt cgcgcccgga
     2101 cacgccgaat ttacgcccgc gcaccgaata gggctggccc atgctgttgt cggaggagcg
     2161 ggcggactgg aatccgggca cataattcat cagttgctcg atcgtgctca ccggcagctg
     2221 gcgaatttcg tcacgggtaa agacggtgac cgaggcaggc acgttgctga gtttcttttt
     2281 cgtcagtgtg gggccggtga tggcgatgtt gcccagttct tccagcgaca gttcaaacag
     2341 gtcctgctgt gtcgcgctga cctgcaccgg acacgccaat gcgataccgg ccagcagggc
     2401 ggccagtgtg gcgaaacgaa acgaatgacg ggtcacgggc atgtagcggc tatccatagt
     2461 ccctggtgca gacatttacc ggttctgact cctttattgt ttaaagcgta gcccaaacgc
     2521 cgctgcccgc aggcggatct cgcgcagaaa atgcgcgatg gaggcgtcgt ccagatcggt
     2581 gtcgttgagc aggggcaggt cgggaaaacg tgcgcggatg cgctgcgcga acaggccgct
     2641 gccctggctg tcgagcacct gctgctgagt gagatcgaga accggagtgg tgttgagacc
     2701 gcagctgggg gaacggcttt gcaggatgta gccgcacagt gtggggtagc tgttggcaaa
     2761 gatgtcagcc acagtgagca ggcggtcggt tacgtcccgg tgtggcgcgt cccgccccag
     2821 ggcgcgaatg ccgtcagcag tccggaccag attgatgggc gggcgcggaa tgcccatgcc
     2881 ggccaccgct tccggacaga acggcagcag gcgacaatag ggcgcgatcc gctcggccac
     2941 cagcaggttg aacttgtcca ggccgtcata gcgcacccgc tgcccggtca ggcagctgct
     3001 gatgcccacc aggggcagat cgtcatcgat agaaggagtc gtcatggcct ggttccagca
     3061 atccatccag ttacagccgc gccggcgcgg tttccatctc gttacccggg agattctgcc
     3121 ccagatcgag cgcgaattgc gaaccctccg catcggcttg gcccatttct tcctgcagca
     3181 cacatcggcg tcactcacca tcaacgaaaa cgccgatccg gatgtgcggg tggattttga
     3241 atcgtacttc aaccggctgg caccggagaa cgcgccctat ttcacccaca cgctggaagg
     3301 accggacgac atgcccgcgc acctgaaatc cagcctgctg ggctgcagcc tcacgctgcc
     3361 cattcagggc ggtgcgctgg cactgggcac ctggcaaggc atttacctgt gcgagcatcg
     3421 caacgagggt ggcagtcgcc ggctgctggt gacactgcag ggggaatagc gcaaaaagcc
     3481 tgcctgctgt aaggtgccgc cacttgtttc agcgaggaat ttcttccatg tctgccatgc
     3541 gtctggttgt gttaaccatg gctctgctga cgaccagctg gctggcgggc ccggtgttgg
     3601 cgcagccgga atctgaaccg gcggtggcca ctgaaccgtc actaggcgat gtcagcctgt
     3661 tcgatccgca ggatggttat ctggatgcca gtgcctggct ggccacccgg ttcggcttcc
     3721 tgccggtgcc ggtgattgtg acggagccag cggtgggcta tggcctgggc ctggcgggca
     3781 tctggtttca tgaaatgccg gtaaaaaagc cctcgggcca gacttacttt ccttccctgt
     3841 ccggcgcggt ctacctggaa accgagaacg aaacccgcat ggccggtggc tttcatatgc
     3901 agtcctggaa agatgacgac tggcgctata cgggatttct ggcccgcgcc gatatcaatc
     3961 tggaaagcca gaccgatctg gagcggccac tgcaatacaa tctgaacgga gacttcctgc
     4021 tgcagcggat cagcctgcgg ctggacgatt tctggcggct gggggcgcat tacctgtatg
     4081 ccggcgggga tgtgcagttc gagcgggagg agctgacgct ggacacggtg agcagcgcgc
     4141 tgggactgca actgtattat gacggcctgg acaacccgtt cagcccgtcc agcggtgcca
     4201 tgctgcagtt cgagcccgag tactttgccg agcaactggg cagtgatgtc agcttcgcca
     4261 aatggaccct ggatgcccag tattacctgc ccctggggcg tcatcgtctg gcgggccgcc
     4321 tggtgggtgg ctgggccgat cagaaggcgc cgttctacaa ccagcctttt atcatgctgc
     4381 ggggtgtgcc caagatgggt tatctgggta ctgaagtggc ggcgattgag gtggaatggg
     4441 cctataacct gcacccacgc tggcagctgc tggcgtttgc cggtgccggt cattccagtt
     4501 cggaaagagg gttgcgggtg gcggagtccg ccagtgtgga ggcaggggga cttggcttcc
     4561 gctacctgct ggcaagccgg ttgggccttc gggtcggtgc cgacctcacg gccaacagcg
     4621 acgacgatta caacctgacg ctggtggtcg gcaccgcctg gttctgatgg tccggtagcc
     4681 cccaatcagt gcatgaatag atttatcagc atgaaaacta acgctttttc tcgcatctgg
     4741 atataattcc gcccgcgcaa attctgtttc ggaatagcgt taatgccctt tggaaataaa
     4801 gatatagatg gctgaatcct tagacaaaaa taccatctaa aaaagcaaaa tatttttagc
     4861 aaagcccgat ggctcgacta taaatatctt tacctctgca agcagccaaa atgattgagg
     4921 attgtgacga tgtctgacta cgatgaaatc gatgaaaccg cagtggaacc cgttgcaggt
     4981 gaagaagcct ctgcactgcc cccggcggag gagccggccc aaatcacatt cgacgatggc
     5041 cgtgaccgta ctgtaagttc ccgcgaagcc gaacgtcgtc gactggaaga tgacatgaaa
     5101 cgcttcctga gcggcggcgg caagattacc caggtcgacc ccaacgtgcg gatggatcct
     5161 ccgcgcaagc cggaatccaa ttacggcagc cggccgatct gatagcaccc acgcttgcag
     5221 gcagggttgt gttgaaaggg ttatgttgaa ctcttgttga ttgcgaagtg ttttttcttg
     5281 aaacccggtt atgactgtaa ccgggttttt tttcgcctgt ggcaacagtc ttagtcgcaa
     5341 ttggaagggg cgggaatgcc ggcgatacgg tcggacaggt agcggagcgc gccgcgatgg
     5401 ccggtgatgg ccagcagcag gtgatcgttg agcacgcgcc ggtactgcac gggcacaccg
     5461 gactcgcagt aatgttccac cactgcatcc acatcccgga tcggaatcag ctcatccagt
     5521 ttgccctggt aaatgtacag tggcgccatc gggacgcgct ggcccagggt attgagcgca
     5581 atcgcccgtt gtacttcggg caagtccagt aactggggca cggtggaata gcggctcatc
     5641 cggcggaagg gatagagcgt cagcgggtcc tgcgtgccgg aaaggaactg cccggcgcag
     5701 gtgttgccaa cccgcg
//
LOCUS       DASUEG010000594         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1982099, whole genome shotgun sequence.
ACCESSION   DASUEG010000594 DASUEG010000000
VERSION     DASUEG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982099"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFX11_11125"
     CDS             complement(<1..628)
                     /locus_tag="VFX11_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX4939220.1"
                     /translation="MPAPTTAGMSELWQRIKFLFIAILVYRIGAHIPVPGIDPIQLQN
                     LFKQQQDTILSLFNMFSGGALERMSILALGIMPYISASIIMQLLQAVTPQLEQLRKEG
                     ESGRRKISQYTRYGTLGLALLQSLGMAMALQSQGVTLSTGFTWLFPAVVTLSTGTMFL
                     MWIGEQVTERGIGNGISMIIFAGIVAGLPQAIGQTLEATRQNELNIAVL"
     gene            complement(662..1096)
                     /gene="rplO"
                     /locus_tag="VFX11_11130"
     CDS             complement(662..1096)
                     /gene="rplO"
                     /locus_tag="VFX11_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX4939221.1"
                     /translation="MRLNSLSPAAGSKPAGKRRGRGIGSGLGKTGGRGGKGQTARQGG
                     SVKPGFEGGQMPIQRRLPKFGFTSQKSLEFQEVRLGELAKVGAEVIDMAALKASGVIK
                     KDTKFAKVVLSGTITRAVTLKGIPATKGAKAAIEAAGGKVEE"
     gene            complement(1097..1285)
                     /gene="rpmD"
                     /locus_tag="VFX11_11135"
     CDS             complement(1097..1285)
                     /gene="rpmD"
                     /locus_tag="VFX11_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX4939222.1"
                     /translation="MSDKTMIKVTQTKSSSGRLKNHRDCVRGLGLRRIGHSVQVEDTP
                     SVRGMINTVYYMVRVEGE"
     gene            complement(1290..1790)
                     /gene="rpsE"
                     /locus_tag="VFX11_11140"
     CDS             complement(1290..1790)
                     /gene="rpsE"
                     /locus_tag="VFX11_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX4939223.1"
                     /translation="MAKVEDKNEGFQEKLVQVNRVAKVVKGGRIFAFTALTVVGDGAG
                     KVGFGSGKAREVPVAIQKALEAARRNMIKVDLNGTTLQHAINAQHGASRVYMQPASDG
                     TGIIAGGAMRAVLEVAGVQNVLAKCYGSTNPMNVVRATVKGLASMKSAEDVAAKRGKT
                     VEEILG"
     gene            complement(1802..2149)
                     /gene="rplR"
                     /locus_tag="VFX11_11145"
     CDS             complement(1802..2149)
                     /gene="rplR"
                     /locus_tag="VFX11_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX4939224.1"
                     /translation="MNEKKVARLRRAKRTRMKIRELGQTRLTVNRTSQHIYAQVISGD
                     GGVVIASASTNEKGYQGPTSNKDAAAAIGKLIAERAKDKGVTHVAFDRSGFKYHGRVQ
                     ALADAAREGGLQF"
     gene            complement(2170..2703)
                     /gene="rplF"
                     /locus_tag="VFX11_11150"
     CDS             complement(2170..2703)
                     /gene="rplF"
                     /locus_tag="VFX11_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX4939225.1"
                     /translation="MSRVAKSPIKVPAGVDIKQVGQQLVVKGSKGELKIALNPLVNVE
                     VAAGVAQIKPVSESQNAWAMAGTFRALVNNMVKGVSAGFERKLELVGVGYRAQAKGKV
                     LSLSLGFSHPVEYQLPDGVTAETPSQTEILLRSSDKQLLGQVAANVRSFRPPEPYKGK
                     GVRYSDEKVLRKEAKKK"
     gene            complement(2717..3109)
                     /gene="rpsH"
                     /locus_tag="VFX11_11155"
     CDS             complement(2717..3109)
                     /gene="rpsH"
                     /locus_tag="VFX11_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX4939226.1"
                     /translation="MSMQDPLSDMMTRIRNGQMAKKPAVSMPSSKMKVAVAKVLQNEG
                     YIKSFAVDGEQKPELTIELKYFEGKPVIEMIKRVSRPGLRIYKPGDQLPKIMGGLGVA
                     IISTDKGVMTDRAARAAGVGGEVLCYVS"
BASE COUNT          669 a          964 c          764 g          721 t
ORIGIN      
        1 acaacactgc aatattcaat tcattctggc gggtggcttc cagcgtctgc ccaatcgcct
       61 ggggcagtcc cgcgacgata ccggcgaaaa tgatcatcga gataccgttt ccaatccccc
      121 gctcagtcac ctgctcaccg atccacatca ggaacatcgt gccagtggag agggtcacca
      181 ctgccgggaa caaccaggta aacccggtcg acagcgtcac tccctggctc tgcaatgcca
      241 tggccatacc cagggattgc agcaacgcaa ggcccaatgt cccataacgg gtgtactggg
      301 aaatcttgcg ccggccggac tcaccttctt tcctgagctg ctcgagctgt ggggtcaccg
      361 cctgcagcag ctgcatgata atcgaggccg agatataggg catgatccca agcgccaata
      421 tggacatgcg ctccagcgca ccgcctgaga acatgttgaa caagctcagg atggtgtcct
      481 gctgctgttt gaacaagttc tgaagctgga ttggatcgat tcctggcaca ggaatatgag
      541 caccaatccg gtaaacgagg atcgcgataa acaggaactt gatgcgctgc cagagctcac
      601 tcattcctgc tgtagtcgga gcaggcatac cggattgctg aggcctgtta gccatgccca
      661 attattcctc tacttttccg cctgctgctt cgatcgccgc cttggcgcct ttcgttgcag
      721 gaattccttt cagagtcacc gcccgggtaa tggtgccgga caatacaact ttagcgaact
      781 tggtatcttt cttgatcaca ccgctggctt tcagtgccgc catgtcgata acttcagcac
      841 caaccttcgc caactcgccc agacgaactt cctggaactc cagggatttc tgggaagtaa
      901 aaccgaactt tggcagacga cgctgaatag gcatctggcc gccttcaaaa cccggcttga
      961 ctgaaccgcc ctgacgggca gtctggcctt tgccaccgcg accgccggtc ttgcccaggc
     1021 cgctaccaat accgcgaccg cgacgtttac ctgcaggctt ggagcctgcc gccggactca
     1081 aactgttcag acgcatttat tcgccctcta cccgaaccat gtaataaacg gtgttaatca
     1141 tgccccgcac agaaggggta tcctcaacct ggacgctgtg gccgatcctt ctgagaccca
     1201 ggccacggac acaatcacgg tggttcttga gccgaccgga actgcttttg gtctgagtaa
     1261 ccttgatcat ggtcttgtct gacatgctgt caccccagta tttcttctac agtcttgcca
     1321 cgcttggcgg cgacgtcttc agccgatttc atgctcgcga ggcccttgac ggtagcacgc
     1381 acgacgttca tgggattggt ggaaccatag cacttggcca atacgttctg cactccggcc
     1441 acttccagca cggcgcgcat agcgccaccg gcgatgatac cggtaccgtc cgaagctggc
     1501 tgcatgtaga cccgagaagc gccatgctgt gcattgattg catgctgcag cgtggtgcca
     1561 ttcagatcaa ccttaatcat gttgcgacgg gcagcttcca gcgccttctg gatagcgaca
     1621 ggtacctcac gggccttgcc actaccaaaa ccgaccttac cagcgccatc gccaacgaca
     1681 gtcagcgccg tgaaggcgaa aatgcgaccg cctttcacta ctttggcaac ccggttcacc
     1741 tgaaccagct tttcctggaa accttcgttc ttatcttcca ctttggccat cttggtaccc
     1801 tttagaactg aagaccgcct tcacgcgcgg catctgccag cgcctgaacg cgaccgtggt
     1861 acttgaatcc ggaacgatcg aacgccacgt gagtgacgcc tttgtctttc gcccgctcag
     1921 caatcagctt gccgattgcg gccgccgcat ctttgttgct ggtcgggcct tgatagccct
     1981 tctcgttggt tgaggcgctc gcaattacga caccaccgtc accggagata acctgagcat
     2041 aaatgtgctg cgacgtgcga ttgactgtca gacgggtctg acccaattcg cgaatcttca
     2101 ttctggtgcg tttcgcccgg cgcaaacgcg ctactttttt ctcgttcatg gctactgtca
     2161 tctcaccggt tatttcttct tggcttcttt ccgaagaact ttctcatcgg aataacgaac
     2221 gcccttgcct ttgtagggct ctggcggacg gaaacttctc acgttggcgg caacctgacc
     2281 gagcagctgc ttgtcagagc tgcgcaacag aatttccgtc tggctcggcg tttcggcggt
     2341 cacgccatcc ggcagttgat actccaccgg gtgggagaaa ccgagcgaca aactcagcac
     2401 tttacccttg gcttgagcac ggtatccaac acctaccagc tccagcttgc gctcaaaacc
     2461 agcgctaaca cctttgacca tgttgttgac cagggcacgg aaagtaccgg ccatcgccca
     2521 cgcattctgg gactctgaca ctggcttgat ctgcgcaact ccggcggcaa cttcaacgtt
     2581 taccaacgga ttcagtgcga tcttcagttc gcccttggaa ccctttacaa ccaactgctg
     2641 tcctacctgc ttgatatcta ctccagcagg cactttgata ggacttttag caactcgaga
     2701 catcgtcggc cacctgtcag gaaacgtaac acaaaacttc gccgccgact ccggctgccc
     2761 gggctgcgcg gtcagtcata acgcccttgt cggtcgaaat gatcgccaca cccaggccac
     2821 ccatgatctt gggcagctgg tcacccggct tgtaaatacg caaaccgggt cggctcacac
     2881 gtttgatcat ctcgatcacc ggcttgcctt cgaaatactt aagctcaatt gtcagttcag
     2941 gcttctgctc gccatcaaca gcaaaggact tgatgtaacc ttcgttctgc agcaccttgg
     3001 caacggcaac cttcatcttg gaagaaggca tggatacagc gggctttttc gccatctgtc
     3061 cgttgcgaat ccgcgtcatc atatccgaca aaggatcttg catactcatc gtctagaa
//
LOCUS       DASUEG010000595         7268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1615750, whole genome shotgun sequence.
ACCESSION   DASUEG010000595 DASUEG010000000
VERSION     DASUEG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615750"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VFX11_11160"
     CDS             <1..1957
                     /locus_tag="VFX11_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939227.1"
                     /translation="GLLQAIEHLSQDRPLSQHNPIIELTIPRLMHRLERVRPTVGTEV
                     NTPDMAFSEHQPLVETLQQLQFRLADQGGLFNDGNQQSLSLLYLALDESSELFNLLGR
                     ARGYGSLYLRTGHVPSDGVIILEQIYDRLISQNEVLQERVQHLVKEGSGLRDVPPFNR
                     NDWLQLDQVADYLDTSVIQAADLELPWRTYFDDISQHVVLLAQARDQLLEQLATIYRA
                     ERSSLQRQQRTYLFALGLLVLVFVVLYVMDLRESGNRLRVRQEKEAAEAADRAKSQFL
                     ATMSHEIRTPINGVLGMVELLADTPLNPEQRDFLAALRSSGQTLMAVLNDVLDFSKIE
                     AGQLQIDNVAFDVRQTLNDCMALFAPQMKQKHLAFHCGVDNDVPALVCCDPSRLRQIL
                     LNLVSNAMKFTEQGSVSVQLSVKKTGEHQYLYGVVRDSGIGMDEAQQAALFKQFSQAD
                     KSIARRYGGTGLGLAICQRLCQLMGGEIGVSSKQGAGSTFWFTLALQPVEGAPVSGAE
                     NVASKDRQRYYTQQLQGRRVLVAEDNRVNQMVVQGMLKKAGIETDVVENGALAVDRIC
                     RQRKTYDAVLMDWEMPEMDGLTACRQIRSWEGDQDLPLTPIVALTAHVLSDYERQAYA
                     AGMQGFLKKPIEQDALYQALLRALHKS"
     gene            1967..3310
                     /locus_tag="VFX11_11165"
     CDS             1967..3310
                     /locus_tag="VFX11_11165"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX4939228.1"
                     /translation="MTRKWLIPLLTAGLLVTAAQAASPARDAERLQRELTPVGAERAG
                     NRDGSIPLWKADQLSDEAHLQWLQEISAEEPLFTINEDNLAQHRPLLSAGMQAMFEHY
                     PDSFAIPVYPARRTARQPQWTYDNTRRNVSDARISPSGDEVLQAWSGTPFPIPHTPHE
                     VMWNHQLRWKGIFIKLHLFETTIFSNQSQTPLETMIETWASFYDRERTSATLEDWRVA
                     YYFSRILGPAKLAGGGLLIHASLQPKQRPMQSWVYLPGERRMRRSPVLGYDSPIFNSE
                     GLRTVDEIDIYNGALDRYDWKLLGKRELYIPYNNQKMRYNRCDDPSALDRGHLKPYTM
                     RFEKHRVWVVDATLKPGLKHLYPRRTFYLDEDTWSIALVDIYDKNGKLWRNTMRFAAY
                     YEAMPGMFSALDAYHDLKANAYFLQCSAGLDTSFHPTAPQPGYFAPASIRQRLKR"
     gene            3509..4357
                     /locus_tag="VFX11_11170"
     CDS             3509..4357
                     /locus_tag="VFX11_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX4939229.1"
                     /translation="MNAIATPPAVQAGWSAELELAFARRGARTLLTHNRHAGPLRVQR
                     PFYPEPGGQCHVYVLHPPGGIVAGDSLHIAAQFDAGAHALLTTPSAGRVYKTNQQRLR
                     QRQTVRLQVDDGAFCEWLPQENILFDGAEGHNSTRIDLQGSARCIGWEITCLGRPASA
                     QWFASGSLLQSLALYRDGVPLLLERNRFMGGSELLAAPWGLQGHATLGTLVISGGDNE
                     LLQQLKELCAQTHNLAVTRLPELILVRGLAHQAAPLRAAFIACWRLARRHFLQVDAVA
                     PRIWFT"
     gene            4405..4707
                     /gene="ureA"
                     /locus_tag="VFX11_11175"
     CDS             4405..4707
                     /gene="ureA"
                     /locus_tag="VFX11_11175"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX4939230.1"
                     /translation="MELTPREKDKLLLFAAAQLAERRKARGLKLNYPEAVALISFEIM
                     EGARDGRTVAELMHHGRTLLSRDDVMDGIPELIHDVQVEATFPDGTKLVTVHNPIV"
     gene            4732..5037
                     /locus_tag="VFX11_11180"
     CDS             4732..5037
                     /locus_tag="VFX11_11180"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX4939231.1"
                     /translation="MIPGELITEPGELELNAGLPKITVMVANTGDRPIQVGSHYHFFE
                     TNAALTFEREKTRGFRLDIAAGTAVRFEPGQSREVTLVPFGGQREVYGFRGLVMGKL"
     gene            5062..6765
                     /gene="ureC"
                     /locus_tag="VFX11_11185"
     CDS             5062..6765
                     /gene="ureC"
                     /locus_tag="VFX11_11185"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX4939232.1"
                     /translation="MAKIDRRAYGDMFGPTVGDRVRLGDTDILIEVEKDFARYGEEVK
                     FGGGKVIRDGMGQSQRPRAECVDTVITNALILDYWGVVKADVGLKNGRIAGIGKAGNP
                     DIQDGVTIVIGPGTEVIAGEGQILTAGGIDSHIHFICPQQIEEALMSGVTTMIGGGTG
                     PATGTNATTCTPGAWHLAQMLKAAEGLPMNLGFLGKGNGSLPEAIEEQIIAGAMGLKL
                     HEDWGTTPAAIDNCLTVADQFDVQVAIHTDTLNESGFVEDTVAAFKGRAIHTYHTEGA
                     GGGHAPDIIKVCGESNVLPSSTNPTRPYTINTVDEHLDMLMVCHHLDANIPEDVAFAD
                     SRIRRETIAAEDILHDLGAFSMISSDSQAMGRVGEVVIRTWQTAHKMKVQRGPLPQDS
                     ERNDNFRAKRYIAKYTINPAITHGIAHEVGSVEVGKWADLVLWKPAFFAVKPSLIIKG
                     GAIAAAPMGDPNASIPTPQPVHYRMMFGGSGRPVQDTSLTFVSQAALEHDIGRRLGLH
                     RPLVAVKGTRTVQKKDLLLNDYQPVMMVDPQTYEVRADGLLLTCEPAKVLPLAQRYFL
                     F"
     gene            6841..>7268
                     /gene="ureE"
                     /locus_tag="VFX11_11190"
     CDS             6841..>7268
                     /gene="ureE"
                     /locus_tag="VFX11_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004744077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX4939233.1"
                     /translation="MKKVFEFAPTGSTATLAICLPFDDRKKSRLRTRTVSGEEIGLIL
                     PRGQVLRHGMLLQDEDGRVIEVQAAPEKVTTALSGNPHVLTRAAYHLGNRHVPLQIGN
                     GWLRYQHDHVLDDMVMGLGLLIKVEQQPFEPEDGAYAGQG"
BASE COUNT         1440 a         2059 c         2352 g         1417 t
ORIGIN      
        1 gggcctgctg caggccatcg agcacctgag ccaggatcgg cctctcagcc agcacaatcc
       61 cattatcgaa ctgaccattc cgcgcctgat gcaccggctg gaacgggtgc gacccacggt
      121 cggcaccgaa gtgaacacgc cggacatggc gttcagcgaa caccagccgc tggtggaaac
      181 gctgcagcag ctgcagttcc ggctggcgga ccagggtggc ctgttcaacg atggcaatca
      241 gcagtcgctg tcgctgctgt atctggcgct ggatgagtcc agtgaattgt tcaacctgct
      301 gggccgcgcc cgtggctacg gcagcctgta tctgcgtaca gggcatgtgc cgtccgatgg
      361 cgtcatcatc ctggaacaga tttacgaccg cctgatcagc cagaacgagg tgttgcagga
      421 acgggtgcag catctggtga aggaaggcag cggcctgcgg gatgttccgc ccttcaatcg
      481 caatgactgg ctgcaactgg atcaggtggc agactatctg gatacgagtg tcatccaggc
      541 ggcggatctg gagctgccct ggcgcaccta cttcgatgac atcagccagc acgtggtgtt
      601 gctggcgcag gcgcgggacc agctgctgga gcagctggcc accatttacc gggccgagcg
      661 ctcgtcgctg cagcgccagc agcgcacgta tctgtttgcg ctgggattgc tggtgctggt
      721 gttcgtggtg ctttacgtga tggatctgcg ggagtccggc aaccggctgc gggtgcggca
      781 ggagaaggag gcagcagaag cagcggatcg cgccaagagt cagttcctgg ccaccatgag
      841 ccacgagatc cgcacgccca tcaacggtgt gctgggcatg gtggaattgc tggcggacac
      901 gccgctcaat cccgagcagc gggattttct ggcggcactg cgcagttccg gccagaccct
      961 gatggcagtg ctcaacgacg tactggattt ttccaagatc gaggccggcc agctgcagat
     1021 cgacaacgtc gccttcgatg tgcggcagac gctgaacgac tgcatggcct tgtttgcgcc
     1081 gcagatgaaa cagaaacatc tggcattcca ctgtggtgtc gacaacgacg taccggcgct
     1141 ggtgtgctgc gacccgtcgc gcctgcggca gatcctgctc aatctggtga gcaatgccat
     1201 gaaattcacc gagcaggggt cggtgtcggt gcagctgtcg gtgaagaaaa ccggcgagca
     1261 ccagtacctg tatggtgtgg tgcgcgattc cggcatcggc atggacgagg cccagcaggc
     1321 ggcactgttc aagcagtttt cccaggccga caaatccatc gcgcggcgtt atggcggcac
     1381 cggtctgggg ctggcgatct gccagcgttt gtgccagctg atggggggcg agatcggtgt
     1441 gagcagcaag cagggcgccg gcagcacctt ctggttcacg ctggcactgc agccggtgga
     1501 gggcgcaccg gtgtcgggtg ccgaaaacgt ggccagcaag gaccgccagc gctactacac
     1561 ccagcaattg caggggcgtc gcgtactggt ggcagaggac aatcgtgtca accagatggt
     1621 ggtgcagggc atgctgaaaa aagcgggcat cgaaaccgac gtggtggaaa acggcgccct
     1681 ggcggtggac cgtatctgcc ggcagcgcaa gacctacgac gccgtgctga tggactggga
     1741 aatgccggaa atggacgggc tgactgcttg ccgccagatc cgttcctggg aaggcgacca
     1801 ggacttgccg ctgacaccga ttgttgcatt gaccgcccat gtgttgtctg attatgaacg
     1861 gcaggcttat gcggcgggca tgcaggggtt tttgaaaaag ccaatcgaac aggacgcgtt
     1921 gtaccaggct ctgctgcggg cgttgcacaa atcctgagga ataataatga caagaaaatg
     1981 gctgattccg ttgctcaccg cagggttgct ggtgacggcg gcgcaggccg cttcaccggc
     2041 gcgggacgcg gaacggttgc agcgggaatt gactccggta ggcgccgagc gcgccggtaa
     2101 tcgcgacggt tccattcctc tctggaaagc ggaccagctc agtgacgagg cccatctgca
     2161 gtggttgcag gagatcagtg cagaagaacc gctgttcacc atcaacgaag acaatctggc
     2221 gcaacatcgt ccactgttgt cggcaggcat gcaggccatg ttcgagcact accccgacag
     2281 ttttgccatc cccgtctatc ctgcgcgacg cacggcgcgt cagccacagt ggacctacga
     2341 caacacccgc cgcaatgtca gtgacgcccg catcagccct tccggggacg aggtgctgca
     2401 ggcatggtcc ggcaccccgt ttccgattcc gcacacgccc catgaagtga tgtggaatca
     2461 ccagctgcgc tggaagggca tcttcatcaa gctgcatctg ttcgaaacca ccatcttttc
     2521 caaccagagc cagacgccgc tggagaccat gatcgaaacc tgggcgtctt tctatgatcg
     2581 cgagcgcacc agtgcgacgc tggaagactg gcgggtggcc tattattttt cccgcattct
     2641 ggggccggcc aaactggcgg gcggcggtct gctgatccac gcgtcactgc aacctaagca
     2701 gcggccgatg caatcctggg tgtatctgcc gggtgagcgt cgcatgcgcc ggtcgccggt
     2761 actgggctac gattccccca tcttcaattc cgaaggcttg cggacggtgg atgaaatcga
     2821 catctacaac ggcgcactgg atcgctacga ctggaagctg ctgggcaagc gcgagctgta
     2881 cattccctac aacaaccaga aaatgcgtta caaccgttgc gacgatccct cggcgctgga
     2941 ccggggccat ctgaaaccct acaccatgcg ttttgaaaag caccgggtgt gggtggtgga
     3001 cgcaaccctc aagcctggtt taaagcatct gtacccgcgc cgcacgtttt atctggacga
     3061 agacacctgg agtattgcgc tggtggatat ttacgacaag aacggcaagc tgtggcgcaa
     3121 caccatgcgc tttgccgcgt actatgaagc catgcctggc atgttctcgg cgctggatgc
     3181 ctatcacgat ctgaaggcga atgcctattt cctgcaatgc agtgccgggc tggacaccag
     3241 cttccacccc acggcgcccc agcctggtta ttttgccccg gccagcatcc ggcagaggct
     3301 gaaacgctga tggcagaatg ttgatgaatg aaccaggaca gggcgttttg ctggtgagcg
     3361 cagcaatttg gtgcagtgaa atgcacgggt tgagggcgca gtattcatgg aaggttgttc
     3421 ggcgtccctg cctgcactca atttccgcgg ccggaatggt tcttgctttt tctgccgcaa
     3481 caatccgcct ggacacggaa ttatccggat gaatgccatt gccactcccc ctgccgtgca
     3541 ggccggctgg tctgccgaac tggaactggc atttgcacgg cgcggcgcgc gtacgctgtt
     3601 gacgcacaac cgccacgccg gaccgttgcg ggtgcagcgg ccgttttatc ccgaacccgg
     3661 tggccaatgc catgtgtacg tgctgcatcc gcccggtggc atcgtggcgg gggacagcct
     3721 gcatattgcc gcgcagtttg acgcgggcgc ccacgcattg ctgaccacgc cttccgccgg
     3781 gcgcgtgtat aaaaccaacc agcagcgtct gcgccagcgc cagaccgtgc ggctgcaggt
     3841 ggatgacggc gccttctgcg aatggctgcc gcaggaaaat atcctgttcg acggcgcgga
     3901 agggcacaat agtacccgca tcgatttgca gggcagcgcc cgctgtattg gctgggagat
     3961 cacctgcctg ggccgaccgg cgtcggcgca gtggtttgca tccggttcgc tgctgcaatc
     4021 gttggcactg tatcgtgatg gcgtgccgtt actgctggaa cgcaaccggt ttatgggtgg
     4081 cagtgaactg ctggcggcgc cctggggctt gcagggtcac gccacactgg gcacgctggt
     4141 gatcagcggc ggcgataacg aattgctgca gcagttgaag gaactgtgcg cgcagacgca
     4201 caacctggcc gtcacccgcc tgccggaact gatcctggtg cggggcctgg cccatcaggc
     4261 ggcgccgttg cgcgcggcct tcattgcctg ctggcgtctg gcacgccggc attttctgca
     4321 ggtggatgcg gtggcgccgc gtatctggtt cacctgaatt tcgctgtgtt ttgaattgac
     4381 gcttgcattg acgagagacc tcacatggaa ctgaccccgc gcgaaaaaga caagctgctg
     4441 ctgttcgccg ctgcgcagct ggcggaacgg cgcaaggccc gtggcctgaa gctgaattat
     4501 cccgaagcag tggcgctgat ttccttcgag atcatggaag gcgcccgcga tggcaggacc
     4561 gtggccgaac tgatgcatca cggccgcacc ctgctgagcc gtgacgatgt gatggacggg
     4621 attcccgaac tgatccacga cgtgcaggtg gaagccactt ttcccgacgg gaccaagctg
     4681 gtcaccgttc acaatccgat tgtgtgaggc gctccgatga atgcgggtgg aatgattccc
     4741 ggtgaactga tcaccgaacc gggtgagctg gaactgaatg caggcttgcc gaaaatcacc
     4801 gtcatggtgg cgaacaccgg cgatcgcccg atccaggtgg gcagccatta tcattttttt
     4861 gaaaccaacg ccgcgctgac cttcgagcgg gaaaaaacgc gtggcttccg gctggatatt
     4921 gcagcgggca ctgcggtgcg gttcgaaccg gggcagagcc gcgaagtgac gctggtgccg
     4981 tttggcggcc agcgggaagt gtatggtttt cgtggcctgg tgatgggcaa actctgagaa
     5041 ttcaatccta aggattcaat catggcgaag attgaccggc gcgcctatgg cgacatgttt
     5101 ggccccaccg ttggcgaccg tgtgcggctg ggggacacgg atatcctgat cgaagtggaa
     5161 aaggattttg cccgctacgg cgaggaagtg aaattcggcg gtggcaaggt gattcgcgac
     5221 ggcatgggtc agagccagcg tccgcgcgcg gaatgtgtcg acaccgtcat caccaacgca
     5281 ctcatcctgg attactgggg cgtggtgaaa gcggatgtgg gcttgaagaa cggtcgcatt
     5341 gccggcattg gcaaagccgg caatcccgac attcaggatg gcgtgacgat tgtgatcggc
     5401 cccggcaccg aagtgatcgc gggtgaaggc cagattctga cggcgggcgg catcgattcc
     5461 cacatccatt tcatctgtcc gcagcagatt gaagaagcgc tgatgtccgg tgtgaccacc
     5521 atgatcggcg gtggtaccgg cccggccacc ggcaccaacg ccaccacctg cacgccgggt
     5581 gcctggcatc tggcgcagat gctgaaagcg gcggaagggc tgccgatgaa tctgggtttt
     5641 ctgggcaagg gcaatggcag cctgccggag gcgatcgagg agcagatcat cgccggcgcc
     5701 atgggattga aactgcacga ggactggggc accacgcccg ccgccatcga taattgtctg
     5761 acggtggcgg atcagttcga cgtgcaggtg gccattcaca ccgacacgct gaatgaatcc
     5821 ggttttgtgg aagacacggt agccgcgttc aagggccgcg cgattcatac ttatcacacc
     5881 gagggtgccg gcggtggtca tgcgccggac atcatcaagg tgtgcggtga aagcaatgtg
     5941 ctgccgtcgt ccaccaatcc cacgcgtccg tacaccatca acaccgtgga cgaacacctg
     6001 gacatgctga tggtgtgtca ccacctggac gcgaatattc ccgaggacgt ggcctttgcc
     6061 gattcgcgca tccggcgcga gacgattgcg gcggaagata tcctgcacga tctgggcgcg
     6121 ttctcgatga tttcgtcgga ttcccaggcc atgggccgtg tcggcgaagt ggtgatccgc
     6181 acctggcaga ccgcgcacaa gatgaaagtg cagcgtggac cgctgccgca ggattccgag
     6241 cgcaatgata atttccgcgc caagcgctac atcgcgaaat ataccatcaa tccggcgatc
     6301 acccacggca ttgcccatga agtgggatcg gtggaagtgg gcaagtgggc ggatctggtg
     6361 ttgtggaagc cggctttttt tgcggtgaag ccgtcgctga tcatcaaggg cggtgcgatt
     6421 gcggcggcgc ccatgggtga tccgaatgcg tcgattccca caccgcagcc ggtgcattac
     6481 cgcatgatgt tcggtggctc gggccggccg gtgcaggaca cgtcgctgac gtttgtgtcg
     6541 caggcggcgc tggagcatga cattggccgg cggctgggtt tgcaccgtcc gctggtggcg
     6601 gtgaagggca cgcgtacggt acagaaaaaa gatctgttgt tgaatgatta ccagccggtg
     6661 atgatggtgg acccgcagac ctatgaagtg cgcgctgatg gtttgctgct gacctgcgaa
     6721 ccggccaagg tgctgccgct ggcgcagcgc tattttctgt tttgacgaag tggtggtgtg
     6781 gcggcatggt tccgcatttg cagaagtctg ctgatcctga aattttgtta aagagatcca
     6841 atgaaaaaag tatttgaatt tgcacccacc ggttccacag ccacattggc gatctgtttg
     6901 ccgttcgatg atcgcaagaa aagccgattg cgcacgcgca cggtgtcagg cgaggaaatc
     6961 gggttgatcc tgccgcgcgg tcaggtactg cgccacggca tgctgctgca ggatgaagac
     7021 ggccgcgtga ttgaagtgca ggccgcgccg gaaaaagtga ctactgcgct gagcggcaat
     7081 ccgcacgtgc tcacccgcgc ggcctaccat ctgggcaatc gccacgtgcc gttgcagatt
     7141 ggcaacggct ggctgcgtta ccagcacgat catgtgctgg acgacatggt gatggggctg
     7201 gggttgctga taaaggttga gcaacagcca ttcgaaccgg aagacggcgc ctatgccggg
     7261 caggggca
//
LOCUS       DASUEG010000596         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1090736, whole genome shotgun sequence.
ACCESSION   DASUEG010000596 DASUEG010000000
VERSION     DASUEG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090736"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="icmH"
                     /locus_tag="VFX11_11195"
     CDS             complement(<1..829)
                     /gene="icmH"
                     /locus_tag="VFX11_11195"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX4939234.1"
                     /translation="MDPNDKTVFTPGQRQPNQSEADRTLFIPTPGARPAAAPTPPPAQ
                     AAPSYAQAAPLPASTELPQLQQGVNPLITASATLLAVLSKLRNTLQHGDVAGLHRQLV
                     DEIKRFDARLKQQNSRPEIALAARYQLCSALDEAVLNTPWGAQAGWSQRSLLSLFHKE
                     TFGGEKFFAILAKMLEAPGTNLEILELNYLLLSLGFEGKYRIDPRGRDRLESLRDNLY
                     STIEKLRGDFPLDLSPRGDSGVKVKKGLVHYLPLWVVTAVVLAILLMSYAGFRVWVYQ
                     "
     gene            complement(834..2168)
                     /gene="tssK"
                     /locus_tag="VFX11_11200"
     CDS             complement(834..2168)
                     /gene="tssK"
                     /locus_tag="VFX11_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX4939235.1"
                     /translation="MTISNKVVWSEGLFLRPQHFQQHDRYLERYIDGRCDAFGGYGWG
                     LLEWSLDTELLKLGKIGIRQARGVFPDGTPFSFPDADELPPILQVPENTSNEMVYLCL
                     PLRRAGAVEVTEPDSQQRLARYVKGTINVRNNTADSAEPAELQVGKLNVRLKLGSEDL
                     SGYARIGIARIQEYIADKSVAIDDGYIPPLLDCAQSPVLDSLLTELHGLLHHRAEALS
                     GRLTDSGRSGSAEIADYLLLQSLNRMEPLVAHFSALHRLHPLTCYTELVQMAGELATF
                     TTRTKRPPEFPNYRHEELQQVFHAVFVSLRQCLSTVLEQTAVALDLAERKYGIHVAPI
                     NDRSMISKSTFVLAVKADMPGNQVRNIFPTQVKIAPVERIREFINAQLPGITLQPLPV
                     APRQIPYHAGFTYFELDRSSDLWKLMESSGGFAIHLGGNFPGLAMELWTIRD"
     gene            complement(2350..2850)
                     /gene="tssJ"
                     /locus_tag="VFX11_11205"
     CDS             complement(2350..2850)
                     /gene="tssJ"
                     /locus_tag="VFX11_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEX4939236.1"
                     /translation="MTKRFMTQHKWHFSGIVIASLLALVLTACGTTRKALDLETTATL
                     KITATGDVNPNSDGRAAPIVIQIIKLRDARQFKQEDFLSLFQDAKGRLGNDYIEMIRL
                     KELAPGESREEKLNLSPDVKFIGILGEYIQYDTAQAKLAIEIVPHKNNEADVRIERLN
                     IRLADE"
     gene            complement(2904..4481)
                     /gene="tagH"
                     /locus_tag="VFX11_11210"
     CDS             complement(2904..4481)
                     /gene="tagH"
                     /locus_tag="VFX11_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEX4939237.1"
                     /translation="MKLVLTVTQSPADNNITGQTFSVDATGATLGRGPANSWVLNDGA
                     RIVSTHHASIGFQQGQFVLTDHSTNGTYVNNAVAALGQGNSTPLQSNDLIRIGKYELR
                     VTIASDAPAARNAIPDSFLDELSNRPPKPAAPPGPVAPSALDDFDKWLEPQSSAAGAG
                     PSWGASNIEYGVTPDVSDEVDPLAAIDQASRGSADPLFTNTLPDDDGSWWKESRSDHA
                     PAINQAFEPARVQRPAAPVPPTTPVVPSGFAPLEPALADGADIDALLGLSTPTQPQPS
                     FEPAEPFASVASGWDAAPARTEPAPARPEPAPSRPAPAPVTPAVARPAIAGSGGDSAR
                     QLADLLGLGALTQEQLDALLPEAASILKESVARLLEGLRARTSIKNEMRLDRTMIQPV
                     ENNPLKFALTDKDALRYLFCADSNAYLSGSRAVKEAFDDINDHQMALLAGMRSAYEYM
                     LARFAPATLESALGDPQSKWFLGNKKSHLWDAYEQYFVKLQQDPEESFNALFGRKFSS
                     TYSEQIDEIKARRRRKS"
     gene            4838..>5136
                     /gene="nadA"
                     /locus_tag="VFX11_11215"
     CDS             4838..>5136
                     /gene="nadA"
                     /locus_tag="VFX11_11215"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX4939238.1"
                     /translation="MTDSVSQFVRYHLDTLHAPPVLDEQQQALYRDRIKQMLKDQNAV
                     LVAHYYTAPELQALAEETGGCVSDSLEMARFGKEHPAKTLVVAGVKFMGETAKIL"
BASE COUNT          932 a         1465 c         1631 g         1108 t
ORIGIN      
        1 actgatacac ccacacccga aaaccggcgt agctcatcag cagaatcgcc agcaccaccg
       61 ccgtcaccac ccacagcggc agataatgca ccagaccttt cttcaccttc acaccggaat
      121 caccgcgcgg cgacagatcc agcggaaaat ccccgcgcag tttttcaatg gtggaataca
      181 gattgtcgcg cagggattcc aggcgatcgc ggccacgggg atcgatgcgg tatttgccct
      241 cgaaacccag gctcagcagc agataattca gctccaggat ttccagattg gtgcccggcg
      301 cttccagcat cttggccaga atcgcaaaga atttttcgcc accaaacgtt tccttgtgga
      361 acaggctcag cagcgaacgc tggctccagc cggcctgtgc gccccagggc gtgttcagta
      421 ccgcctcgtc cagcgccgag cacaactgat agcgtgcagc caatgcaatt tccggccggc
      481 tgttctgctg cttcaggcgc gcgtcaaagc gtttgatctc atccaccaat tgccggtgca
      541 atcctgccac gtcaccgtgc tgcagcgtat tgcgcagctt gcttagcacc gccagcaggg
      601 tggcggacgc agtaatcaac gggttcacac cttgctgcaa ttgcggcagt tccgtgctgg
      661 caggcaatgg cgctgcctgc gcgtaagaag gtgcggcctg ggcgggcggt ggcgtgggtg
      721 ctgctgctgg ccgtgcaccg ggtgtcggaa tgaacagggt ccggtccgct tcggactggt
      781 tgggttgccg ctggcccggt gtgaaaacgg tcttgtcgtt cggatccatc gtatcagtcc
      841 cttatggtcc agagttccat ggccaggccc gggaagttgc cgcccaggtg aatcgcaaat
      901 ccaccggagc tttccatgag cttccacaga tcgctggagc gatccagttc aaaataggtg
      961 aagccggcat gatagggaat ctggcgcggt gccaccggca atggctgcag cgtaatgccc
     1021 ggcagttgcg cgttgatgaa ttcgcggatg cgttccaccg gcgcgatttt tacctgggtc
     1081 gggaaaatat tgcgtacctg attgcccggc atgtcggctt tgacggccag cacaaaagtg
     1141 gacttgctga tcatggagcg gtcgttgatc ggtgccacat ggataccgta tttgcgttcc
     1201 gccagatcca gcgcgacagc ggtctgttcc agcacggtgc tcaggcactg acgcaggctg
     1261 acgaacacgg catgaaatac ctgctgcaat tcttcatggc gataattggg aaattccggc
     1321 ggccgtttcg tgcgggtggt gaaggtggcc agctcaccgg ccatctgcac cagttcggta
     1381 tagcaggtca atggatgcag gcggtgcagc gcgctgaagt gagccaccag cggctccatc
     1441 cggttaagcg attgcagcag cagataatca gcaatttcag cagagccgga gcggccgctg
     1501 tcggtcaggc gtccgctgag cgcctcggca cggtgatgca gcaggccgtg cagttcggtg
     1561 agcaggctgt ccagcaccgg tgactgggca caatccagca gcgggggaat atagccgtcg
     1621 tcgatcgcca ccgatttgtc cgcaatgtat tcctggatgc gcgcgatgcc gatgcgggca
     1681 tagccactca gatcctccga ccccagcttc agccgcacat tcagtttgcc cacctgcagc
     1741 tctgccggct cggcgctgtc tgccgtgttg ttgcgcacgt tgatcgtgcc tttgacatag
     1801 cgtgccagtc gctgctggct gtcgggttcg gtgacttcca cggcgccggc gcggcgcagt
     1861 ggcaggcaca gatacaccat ttcgttactg gtattttcgg gaacctgcag gatggggggc
     1921 agttcgtcgg cgtcgggaaa gctgaagggc gtaccgtccg gaaacacgcc ccgggcctga
     1981 cggatgccga tcttgcccag cttcagaagt tcggtgtcca gcgaccattc cagcaggccc
     2041 cagccgtagc cgccgaaggc atcgcagcgg ccgtcgatat agcgttccag gtagcggtcg
     2101 tgctgctgaa agtgctgggg gcgtaaaaac aatccctcag accagaccac cttgttgctg
     2161 attgtcattc ggggctccgg tgggaatggg cgtcaatgcc gctgttttga tggttgaacc
     2221 ggttgggcca atgagcacgc ttgatttgca gtgcctttaa acaattttcc agcaggtaag
     2281 gcgcaggttt caacgcccgc tggccggtct ggtattttta ccggcggggc gggcgggccg
     2341 taatcggtat tattcgtccg ccagacggat gttgagacgc tcgatccgca cgtccgcttc
     2401 gttgttcttg tggggcacga tctcgatcgc cagcttggcc tgcgccgtgt cgtactggat
     2461 gtactcgccc aggatgccga tgaatttcac gtccggtgac aggttcagct tttcttcccg
     2521 gctttcgccc ggtgccagct ctttcagtcg gatcatttca atgtaatcgt tacccagccg
     2581 gcccttggcg tcctggaaca ggctgaggaa atcctcctgc ttgaactggc gcgcgtcccg
     2641 cagcttgatg atctggatga cgatcggtgc ggcgcggccg tcggaattgg ggttcacgtc
     2701 accggtggcg gtgattttca gggtggcggt ggtttcaagg tccagtgcct tgcgggtggt
     2761 gccgcacgca gtcagtacca gtgccagcag gctggcgata acaatgccgg agaaatgcca
     2821 tttgtgttgc gtcatgaaac gtttggtcat gatgtccatc gtcgctgtat gtggaaaacg
     2881 ggaagatccg gtggtgcctg ccgtcagctt ttccggcgcc gccgcgcctt gatctcatcg
     2941 atctgttcac tgtaggtgct ggaaaacttc cgcccgaaca gcgcattgaa gctctcttcc
     3001 ggatcctgct gaagcttaac aaaatattgt tcgtacgcat cccacagatg cgatttttta
     3061 ttgcccagga accacttcga ttgcgggtcg ccgagggcac tttccagggt ggctggcgcg
     3121 aagcgggcca gcatgtattc gtaggcactg cgcatgccgg ccagcagggc catctggtga
     3181 tcgttgatgt cgtcgaaggc ttccttcacc gcgcggctgc cggacagata agcgttgctg
     3241 tcggcgcaga acaggtagcg caacgcatcc ttgtcggtga gggcgaactt gagcggattg
     3301 ttttccaccg gctggatcat cgtgcggtcc aggcgcattt cgtttttgat gctggtgcgg
     3361 gcccgcagtc cttccagcag gcgggcaacg gattccttca aaatgcttgc agcttcgggc
     3421 aacagtgcgt ccagctgttc ctgggtcagt gcgcccagcc ccagcagatc ggccagctgg
     3481 cgcgcactgt cgccgccgct tcccgcaatc gcggggcgtg ccaccgccgg tgtgacgggg
     3541 gcgggtgccg gtctggatgg tgcgggttcc gggcgggcag gtgcgggctc agtcctggcg
     3601 ggagcggcat cccagccgga tgcaacggat gcaaacggct cagccggttc gaacgatggc
     3661 tggggctggg tcggggtgct caggccgagc agtgcgtcga tgtcggctcc gtcagccagg
     3721 gccggttcca ggggcgcgaa tccggacggt acaacgggag ttgtgggagg cacgggcgct
     3781 gccggtcgct gcacgcgagc gggttcaaac gcctggttga tagcgggcgc atggtcggaa
     3841 cggctttcct tccaccaact gccatcgtcg tccggcagcg tattggtgaa cagcgggtct
     3901 gccgatcccc gcgaagcctg atcgatggcg gccagcgggt ccacttcgtc gctgacatcc
     3961 ggtgttacgc cgtattcgat gttggacgcg ccccaggagg gtccagcccc cgctgcgctg
     4021 gactggggtt ccagccattt gtcgaaatca tccagtgcag acggtgccac cggtccgggt
     4081 ggtgccgccg gtttgggcgg ccggttcgac agctcgtcca gaaaactgtc ggggatggcg
     4141 ttgcgtgcgg cgggagcgtc gctcgcgatg gtcacccgca attcgtattt gccgatgcgg
     4201 atcagatcgt tgctttgcaa tggtgttgaa ttgccctggc ccagggcggc gacggcgttg
     4261 ttgacatagg tgccgttggt gctgtgatcg gtcaggacaa actggccctg ctggaatcca
     4321 atgctggcat ggtgcgtgga tacgatccgc gcgccatcat tcaagaccca gctgttggcg
     4381 gggcctcggc caagagtcgc gccggtggca tcgacactga acgtctggcc ggtgatattg
     4441 ttgtcggcag gcgactgggt gacggttaac actagtttca tcgtcgtctt tgtcttgtgt
     4501 gggccggtgt tggacgttgg cgggctatga catcatgagt ccagaggcgt gcgaacctaa
     4561 aatagcccaa ttgatctttc aattctagct gtagtggcgc cgattgcagt attcaggcag
     4621 gatctaaatg aatgaaattc aaaaggtaaa tcggaagctg ccgattccgt ggccagaacc
     4681 gccctttaaa cgggcttcag gtgcccccat catttgtgca gtgccatcgc agtgaaagcg
     4741 gtgtgatatg atggcccgcc tttaatgcat ggccgccggg cagcggtggc atgcgctggt
     4801 aacgatgcca ggattatgcc tgttccgagg tttccccatg actgattccg tttcccaatt
     4861 tgttcgctat cacctggaca cgctgcacgc gccccccgtc ctggatgaac agcagcaggc
     4921 cctgtaccgc gaccgaatca agcagatgct gaaggaccag aacgccgtgc tggtggcgca
     4981 ttattatact gcgcctgaac tgcaggccct ggcggaggag actggcggtt gcgtgtctga
     5041 ctcgctggaa atggcccgct tcggcaagga gcatcccgcc aaaaccctgg tcgtggccgg
     5101 cgtaaaattc atgggcgaga cggcaaagat tctcag
//
LOCUS       DASUEG010000597         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1650877, whole genome shotgun sequence.
ACCESSION   DASUEG010000597 DASUEG010000000
VERSION     DASUEG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650877"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFX11_11220"
     CDS             complement(<1..392)
                     /locus_tag="VFX11_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939239.1"
                     /translation="MHRRSIQCLTLSGFLLTFVASTTFADEDLLIHEQWALNNVPRTA
                     QNSSALITTANTATIGTCADKPVISGSQYSCSEYNAPGVEDMDINAPEGWSQYSPSPT
                     LAQNEIVIALVDTGIDYTHPDLRDRIWL"
     gene            complement(700..1929)
                     /locus_tag="VFX11_11225"
     CDS             complement(700..1929)
                     /locus_tag="VFX11_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX4939240.1"
                     /translation="MEFIDYLKYFAERDGSDLYLSTGAPPSAKFYGKLEPIDKDPLPP
                     GRVKEIAYSIMNEEQKKDFEKKPELNLAISEPGVGRFRVNIFKQRNQLAMVCRAIKTD
                     IPNYRDLGLPDILPKLVMQKRGLILFVGGTGSGKSTSLAALIDYRNSNSAGHIITIED
                     PVEFVHRHKMSIVNQREVGMDTNCYDDALENTLRQAPDVILIGEIRTQEQMEQAIAFA
                     ETGHLCISTLHANNANQALDRIINFFPEERHKQLFLDLSLNMRGIVSQRLIPTVDGKR
                     CAAIEILLSTPRVADLIKQGKVDEIKEVMLKSEAQGMQTFDTALYKLYKDGKISLDEA
                     LKNADSKNNLRLRITLEENPGSLTKEQVAKMSEPEPASQKQEKAAPSVPSKLTELSLE
                     PIADPEEENNRPAFKPR"
     gene            2328..>2570
                     /locus_tag="VFX11_11230"
     CDS             2328..>2570
                     /locus_tag="VFX11_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX4939241.1"
                     /translation="MPVLQEITKGKVPVRIYTRDIEPQAYQQLANIANLPIVHGHIAA
                     MPDVHLGKGATVGSVIPTRNAIIPAAVGVDIGCGMNA"
BASE COUNT          545 a          682 c          732 g          611 t
ORIGIN      
        1 ttcagccaga tgcgatcgcg cagatccgga tgggtgtaat cgatcccggt atccaccagc
       61 gcaatgacaa tttcattctg ggccagtgtc ggggagggtg aatactggct ccagccttcc
      121 ggcgcattga tgtccatgtc ctcgacaccg ggagcgttgt attcggagca gctgtactga
      181 ctgccgctga tcaccggttt gtctgcgcag gtgccgattg tggcggtatt cgcggtggtg
      241 atcagcgcgg aagagttttg tgccgtacgg ggaacattgt tgagggccca ctgttcgtgg
      301 atcagcagat cctcatcggc gaaagtggtg ctggccacga acgtcagcag gaatccggac
      361 aacgtgagac attgaatact acggcgatgc atatagcgac ctctgtcaag acaaatacgg
      421 gaaagccagt cgatgtcctg aacgttgcag ccgtgataac accaccgcac gatgggcatc
      481 gaagtccgct ttcgctatct tgagaaataa ttcctgccga taaattacca ctggctgccg
      541 cgctggggtc ggcacatacc atgaacgtca caggaagaat ttccgttcca ctgtacagtt
      601 cgtgaccgaa agcacagtac atacggaaat ggtagacgca gtttctggaa cctgcaggga
      661 aaaaagatga attaattatt tcagaaggac aagccgcgct cagcgcggct tgaatgcagg
      721 gcggttgttt tcttcttccg gatcggcgat aggctcgagc gataattctg tcaatttcga
      781 cggcacactc ggcgccgctt tttcctgttt ttgtgatgca ggttccggtt cgctcatctt
      841 ggccacctgc tccttggtga gggaccccgg attttcttcc agtgtgatgc gcagtcgcag
      901 gttgttcttc gagtccgcat tcttcagcgc ttcatccagt gaaatcttgc cgtccttgta
      961 cagcttgtag agcgctgtgt cgaacgtctg catgccttgc gcttcggatt tcagcatgac
     1021 ttccttgatc tcatccacct tgccctgctt gatcagatcg gcgacgcggg gagtggagag
     1081 gaggatttca atggccgcac agcgcttgcc gtcaacggtg ggaatcaggc gctgcgacac
     1141 gataccgcgc atgttcagcg acagatccag gaacagctgc ttgtggcgtt cctcggggaa
     1201 gaagttgatg atgcgatcca gcgcctggtt ggcgttgttg gcatgcaggg tggaaataca
     1261 caggtggccg gtttcggcga aggcaatcgc ctgttccatc tgttcctgcg tgcggatctc
     1321 gccgatcagg ataacgtccg gcgcctggcg cagggtgttt tccagggcat cgtcgtagca
     1381 gttggtatcc atgcccactt cgcgctggtt gacgatcgac atcttgtgcc ggtgcacgaa
     1441 ttccaccggg tcttcaatgg tgatgatatg gcccgcgctg ttggaattgc ggtaatcgat
     1501 taacgccgcc agtgaggtgg atttgccgga gccggtaccg cccacgaaca ggatcaggcc
     1561 gcgcttctgc atcaccagct tgggcaggat gtccggcagg cccaggtcgc ggtagttggg
     1621 aatatccgtc ttgatggccc ggcagaccat ggccaactga ttgcgctgtt tgaagatatt
     1681 gacccggaac cggcccacac ctggctccga gatcgccaga ttcagctccg gctttttctc
     1741 gaaatccttc ttctgttcct cgttcatgat gctgtacgcg atttccttga cgcgtcccgg
     1801 cggcagcgga tccttgtcga tcggttccag cttgccgtaa aactttgcgc tgggcggagc
     1861 gccagtggac aggtacaggt cggaaccatc gcgctcggcg aaatacttca ggtaatcaat
     1921 gaattccatg gggaatttcc tgtcacaggg gtgttgcatc gggaatgtag ccacttgagt
     1981 atagccaacc gatgcggtgt cgccggcact ggtttaatca catccgttat aagagaaacc
     2041 gcttctatgc ccaatatttt tcagcacagg cattgacttg tgacatgctg aatttagaat
     2101 gcgcgccaca gagtcccgtg ttggcgcggg ccaattactt gttatatgta aggaggttcg
     2161 aaagtggcat atcaggtggt atatccaatg acaaccaaac gtaactaacc gctttcgaat
     2221 tccgattcgg aagcgacacc aaccgcttcc gagtcaggca tcctgaaaac cctgacttgc
     2281 acatgtcgtc gcagtcaggg tttttttatg ctcgcaggag aaagaacatg cctgttttac
     2341 aggaaataac caaaggaaaa gtgccggtgc gcatttacac ccgcgacatc gagccgcagg
     2401 cgtaccagca gctggccaac atcgccaacc tgcccatcgt gcacggacat atcgccgcca
     2461 tgcccgatgt gcatctgggc aagggcgcca cggtgggatc agtgattccc acccgcaacg
     2521 ccatcattcc cgctgcagtg ggcgtggata tcggttgcgg catgaatgca
//
LOCUS       DASUEG010000598         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1450871, whole genome shotgun sequence.
ACCESSION   DASUEG010000598 DASUEG010000000
VERSION     DASUEG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450871"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFX11_11235"
     CDS             <1..788
                     /locus_tag="VFX11_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4939242.1"
                     /translation="YRGQVLEYLFTSSPTFGDGLKRAMAFHRLLSDVVAGRLVVDDQH
                     CYLVGMAGPRANRHFAECLAAGMIQFFRFVTDNHFQPLGVHFEHAEGAPKEDYERIYG
                     CPATLGCAEYRLYFNREVLDHRIWQAEPRLLRLHEQLANEQLAELERHDLVSEVKRAI
                     GEMLESGDTSLESVAKRLGLPPRRLRSQLAEANTSFNQILADYRSRLSRRLLARTDEP
                     IEQIVYLTGFSEPSTFYRAFKRWTNETPVEYRKRKREQAAARR"
     gene            complement(789..>2154)
                     /locus_tag="VFX11_11240"
     CDS             complement(789..>2154)
                     /locus_tag="VFX11_11240"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEX4939243.1"
                     /translation="TERSDEKQARVLIAQCRDTLLKVRKHAPSTGVSISLTQLLVRIS
                     QMLDRIEHLLCLLEAEGNERHLVIVQLFKELVTADNRKYSLRDLFSTHTELLALLVTE
                     NAGHTGEHYVTTTRKGYFGMLRSGMGAGLIVGFMAMLKVLAGKLSLAPLAAAFVYSMN
                     YSLGFMLVHVLHFTIATKQPAMTASRIAASISDAQKGKEQNLDGLADLCVNVFRTQFI
                     AIVGNVALSIPTAYAIAWIWRWSTGVHLASPDKVAHLVHDLSPIESLAIFHAAIAGVC
                     LFLSGLISGYYDNKCLYNRIPERLQQRPLLQRLLGQERLARFSGYIGNNLGALAGNFY
                     FGLMLGSMGTIGFILGLPLDIRHITFSSAYFSFALVGSDHQLPLQTIAISLVGVAAIG
                     MTNLLVSFSLALMVALKSRRVRYRQWWPLTVLILKRFLKQPGHFFWPPRETRESTEQP
                     ARQD"
BASE COUNT          444 a          601 c          655 g          454 t
ORIGIN      
        1 tctatcgtgg ccaggtgctg gagtacctct tcacttccag tcccacgttc ggtgatggcc
       61 tcaaacgtgc catggcattc caccgtctgc tcagcgatgt cgtggctggc cggttagtgg
      121 tggatgacca gcattgctat ctggtgggca tggcaggccc gcgtgccaac cggcactttg
      181 ccgaatgtct ggcggccggc atgatccagt tcttccgctt tgtcacggac aatcattttc
      241 agcccttggg cgtgcacttt gagcatgcag agggagcccc caaagaagat tacgagcgca
      301 tatacggctg tcctgccaca ctgggctgcg ccgagtatcg cctgtatttc aatcgtgagg
      361 tgctggatca ccgcatctgg caggccgagc cacgattgtt gcggctgcat gagcagctcg
      421 ccaacgagca gttggctgag cttgaacgcc acgacctggt ctccgaagtg aagcgtgcga
      481 tcggtgaaat gctggaaagc ggtgatacca gtctggagtc ggtggccaag cgccttggtc
      541 tgcctccccg ccgcctgcgt agccaactgg ctgaagccaa taccagtttt aaccagattc
      601 tggcggacta tcgctctcgc ctgtcccgtc gtttgttggc caggacggat gagcccattg
      661 agcagattgt ttatctcacc ggcttctccg agccgagtac tttctaccgt gccttcaagc
      721 gctggaccaa cgaaacaccg gtcgaatacc gcaagcgcaa gcgtgagcaa gcagctgcaa
      781 ggcgctgatc agtcctgacg ggctggttgc tcggtgcttt cgcgagtttc cctgggtggc
      841 cagaagaagt ggcctggctg tttcaggaag cgtttcagga tcagtacagt cagtggccac
      901 cactgacgat agcgtacccg ccgtgatttc aatgcaacca tcagggccag tgagaaactc
      961 accagcaggt tggtcatgcc gatggcggca acgccgacca ggctgatcgc aatcgtctgc
     1021 aggggaagct ggtgatctga tccgaccagt gcaaacgaga agtaggcgga tgaaaaggtg
     1081 atgtgccgta tatccagggg caagcccaga atgaagccga tggtgcccat gctgcccagc
     1141 atcagaccga aatagaagtt acccgccagc gccccaagat tattgccgat atagccgctg
     1201 aagcgggcca gtcgttcctg gcccagcagt cgctgcaaca gtggtcgttg ctgcagacgc
     1261 tccgggatgc ggttgtacag gcacttgttg tcgtaatagc ccgagatcag gccggacagg
     1321 aacaggcaga caccggcgat ggcggcatgg aaaatcgcga ggctctcgat cggactcagg
     1381 tcgtggacca gatgggcaac cttgtcgggg ctggcgagat gcacaccggt cgaccaccgc
     1441 catatccatg caatcgcata cgctgtcgga atcgaaagcg ccacattgcc gacgatagcg
     1501 atgaactgtg tgcggaagac gttgacgcaa aggtccgcca gtccgtccag gttctgctcc
     1561 ttgcctttct gggcgtcact gatgctggca gcaatcctcg atgcggtcat ggccggttgc
     1621 ttggtggcga tggtgaaatg caatacatgc acgagcatga agccgagcga gtagttcatg
     1681 ctgtagacaa aggcggcagc gagcggtgcc agtgatagtt tgccggccag aaccttgagc
     1741 atggccatga agccaacgat tagtccggca cccatgccag agcgcaacat gccgaaatag
     1801 cctttacgcg tggtggtcac atagtgctcg ccggtgtggc cggcattttc agtgaccagc
     1861 agggccagca actcggtgtg ggtagagaac aggtcgcgca agctgtattt gcgattgtcg
     1921 gcggtcacca gctccttgaa tagctggacg atgacgaggt gtcgctcgtt accctctgcc
     1981 tccaggaggc agaggaggtg ctcgatgcgg tccagcatct ggctgatgcg caccagcaat
     2041 tgggtcaggc tgatgctcac gcccgtgctg ggcgcatgtt tgcgcacttt caggagggta
     2101 tcccgacact gtgcaatcaa tacccttgcc tgtttttcat cgctgcgctc tgtc
//
LOCUS       DASUEG010000599         5407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1041334, whole genome shotgun sequence.
ACCESSION   DASUEG010000599 DASUEG010000000
VERSION     DASUEG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041334"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..290
                     /locus_tag="VFX11_11245"
                     /pseudo
     CDS             84..290
                     /locus_tag="VFX11_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
     gene            316..1089
                     /locus_tag="VFX11_11250"
     CDS             316..1089
                     /locus_tag="VFX11_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939244.1"
                     /translation="MGKNSGKIFAFTLLALQLPAANAEPWQFDVTPYFFAAAMDATAD
                     VGPVKTDVDMSASDVLEDTQNGFMAMMSARKDRWVVGLDASYFKLGDGQSESVTGPFG
                     RTTVEGEVELTDRQWVYQPMLGYQVLDQGMQAHVYGGARYTRLRANLNVVIDTSIAAF
                     PGGERSAKAEKEWTDFVVGGHLSLPLAERWSIESMADVGKGDDSDISYHFLVAAKWQF
                     HPMMAASAGYRLLHQEYEDDTFAWDVTCSGLIMGVGFSF"
     gene            complement(1113..2594)
                     /locus_tag="VFX11_11255"
     CDS             complement(1113..2594)
                     /locus_tag="VFX11_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX4939245.1"
                     /translation="MPTNIPVQPSYAVSDSADTRLARFSQPLTQELQPDQSGFRQLGD
                     GTEALLARLALAEMADKALDVQYYIWRDDLTGRHFANALLKAADRGVRVRVILDDIGA
                     NADDDVLAAIDLHPNIEIRLFNPVASRTFRGLSMLGDFSRTNRRMHNKAMIADNQAAI
                     VGGRNIGDEYFDASGDVAFGDLDLISVGPVVRQVSDSFDRYWNSRSVMPIAALHTVDV
                     AQLAQLRTDLAAFIQEQRQSPYVQQSLRYLDEKIASGPDTLFRGQAFVLYDDPAKISQ
                     SPENIEGSLITQFSRLGGSIQHELFIISPYFIPGDQGVAWLRGLAQRGVAITILTNSL
                     AASDVSAVHAGYRRYRQQLLQAGIRLYELKPGAVERASELKKHRVGASRASLHAKTFI
                     FDRSAVFIGSLNLDPRSIQLNTEVGIVCINADMAQQLDQRLRQNLDHIAWRVQLSPDD
                     KGRLSWHETGSDGDTVYHDEPQTSVWRRMSVWFVGLLPLESQL"
     gene            complement(2977..4005)
                     /gene="cmoB"
                     /locus_tag="VFX11_11260"
     CDS             complement(2977..4005)
                     /gene="cmoB"
                     /locus_tag="VFX11_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HEX4939246.1"
                     /translation="MSMIDPAGANSTPSSFDPMQEFARFAETMRRSALAPWTDAFMHT
                     LQQQFVPDQHGDFARWQRCVEALPPVKVSQCDFAADSVTVGVPGDCDDATREKIETAL
                     RGLHPWRKGPFNVCGIQIDTEWRSDWKWQRLQPHIQSLQNRTVLDIGCGSGYHLWRML
                     GEGAALTIGIDPSLLFTRQFRAIEHFTGPQPVFHLPLGIEHLPPDLHAFDTVFSMGVL
                     YHRRSPLDHLLELRELLRPGGELVLETLIVEGPEGFSLVPEGRYGRMGNVWFLPSVPT
                     LLAWLKKVRFRNVRCVDVTPTTVQEQRPTDWMRFQSLPDFLDPNDSSKTIEGYPAPVR
                     AIVVAEAP"
     gene            complement(4015..4752)
                     /gene="cmoA"
                     /locus_tag="VFX11_11265"
     CDS             complement(4015..4752)
                     /gene="cmoA"
                     /locus_tag="VFX11_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011072410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEX4939247.1"
                     /translation="MTDSSDKDNLFARPKPHLVDFAFDEQVARVFPDMIRRSVPGYDT
                     VIAMLGVFASHYVTPHSRVYDLGCSQGAATLALRRHIQVPDVTLVGVDNSSAMIERCQ
                     HNIAADLSPSRVELRCEDIQDTPLENASLVALNFTLQFLPPEQRDAMIGKVARGLNPG
                     GVLVLSEKICFDAVEQQSLFTDLHHEFKRLNGYSDLEISQKRQAIENVLIPDTAEQHR
                     QRLLRNGFSQVHCWFQCFSFASFLAVK"
     gene            complement(4885..>5407)
                     /locus_tag="VFX11_11270"
     CDS             complement(4885..>5407)
                     /locus_tag="VFX11_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939248.1"
                     /translation="VDAVGLSPMATKIVHSTVDIIDKTAKMLVGQLLKKRSNEELKPL
                     IKFVDELFVRAEIASTGKASTGCEIDQPTYDRIVRVIAEVRAGNGQQVLPELHDLMSL
                     VVDIQVEQLMKRSIAALPLNFIVKKVADGAIATCRAAGHGVVNKVFKKLEEEQLIHLA
                     NHFDTLLLSAERN"
BASE COUNT         1177 a         1590 c         1584 g         1056 t
ORIGIN      
        1 cgggagagca gtagcggggt gttcgacgtg ctgtggaaag tgccaagccg cggtgagtgg
       61 caattaaatc tcacgcccgc attgcgcctg agttgatcgc cgcctgggaa gatccagact
      121 ttgatgccaa ccaacctgcg ttttactacg gccgcgtact ggaaattccc acaccgcgct
      181 ggaccgccta cgacgccaaa cggtttggag cgaaacctct ggaaggcacc accatgacct
      241 tgcaggaacg cgcttacaca tcgcctatct ggtatcaccc cgagaaataa acaattacag
      301 cgcaaggaac aggacatggg caaaaacagc ggcaagatat tcgcttttac gctactcgca
      361 ttgcaattgc cggcggcaaa tgccgaaccc tggcaatttg atgtcacacc ctattttttt
      421 gcggcggcca tggatgcaac ggctgatgtg ggtccggtaa aaacggatgt cgacatgtcg
      481 gccagtgacg tgctggagga tacccagaat ggattcatgg cgatgatgtc cgcacgcaag
      541 gatcgctggg tggtggggtt ggatgcttcc tatttcaaat tgggggacgg ccagtccgaa
      601 tcagtcaccg gacccttcgg acgcaccacc gtggaaggcg aggtcgagct gactgaccgg
      661 caatgggttt atcaacccat gctgggctat caagtgctcg accaggggat gcaggcacac
      721 gtatacggag gagcgcgcta tacccgcctg cgcgccaatc tgaacgtggt catcgacacc
      781 tccatcgcgg cctttcccgg cggagaacga agtgccaaag ccgaaaaaga atggacggat
      841 tttgtggtcg gtggccacct tagcctgccg ttagccgaac gctggagcat cgaaagcatg
      901 gccgacgttg gcaaaggcga cgactccgac atcagctatc acttcctggt ggcggccaaa
      961 tggcaatttc atccaatgat ggcggccagt gccggttacc ggctgctgca tcaggaatac
     1021 gaagacgata ccttcgcctg ggatgtgacc tgtagcggcc tgatcatggg cgttggcttc
     1081 agcttctgac ttgtgagcaa aaaaatctcg cgctacaact gcgattcgag cggcagcaag
     1141 ccaacgaacc acaccgacat gcggcgccag acgctggttt gtggttcatc gtgatacacc
     1201 gtgtcaccat cgctgccggt ctcgtgccag cttagccggc ccttgtcgtc gggcgacagt
     1261 tgcacgcgcc aggcaatgtg gtcgagattt tgccgcagcc gctgatccag ttgctgcgcc
     1321 atgtcggcgt tgatgcaaac aatacccacc tcggtgttga gctggatcga gcgtgggtcg
     1381 agattgagcg agccgatgaa cacggcactg cgatcaaaaa tgaaggtttt ggcgtgcaat
     1441 gatgcgcgcg atgcgccaac gcggtgtttt ttcagttcgc tggcccgttc cactgcaccc
     1501 ggtttgagtt catacagccg gatgccagcc tgcagtaatt gttgccgata gcgacgataa
     1561 cctgcgtgca ccgcacttac atcggaagcg gccagcgagt tggtgagaat ggtgatggcg
     1621 acaccgcgct gtgccagccc gcgcaaccag gccacgcctt gatcaccggg gatgaagtag
     1681 ggcgagatga tgaacagctc gtgttgaatc gacccgccaa gccgggaaaa ttgcgtgatc
     1741 aggctgcctt cgatattctc cggtgactgg ctgatttttg ccggatcgtc gtaaagcaca
     1801 aaggcctgac cacggaagag cgtgtccgga ccggacgcaa ttttctcgtc aaggtagcgc
     1861 aggctttgct gcacataggg actttgccgc tgctcttgga tgaaggccgc cagatcggtg
     1921 cgcagctgcg ccagttgcgc cacgtccacc gtgtgcaagg cagcgatcgg cattaccgag
     1981 cgactgttcc agtagcgatc aaacgaatca gatacctgac gcaccaccgg accgacgctg
     2041 atcaggtcca gatctccaaa cgccacatcg cccgaggcat caaaatattc gtcgccaata
     2101 ttgcggccgc cgacaattgc ggcctgatta tcggcaatca tcgccttgtt gtgcatgcgg
     2161 cgattagtgc gggagaaatc gcccagcatg ctcagaccgc gaaacgtgcg cgaggccacc
     2221 gggttgaaca gacggatttc aatattggga tgcagatcaa tggcggcgag gacgtcatcg
     2281 tcggcattgg caccgatatc gtccagtatc acccgcaccc gtacaccgcg atctgcggcc
     2341 ttgagcagcg cattggcaaa gtggcggccg gtgagatcgt cgcgccagat gtaatactgc
     2401 acatccagcg ctttgtcggc catttctgcc agcgcgagcc gtgccagcag cgcttcggtg
     2461 ccatcaccca gctgccggaa gccagactga tcaggctgca gttcttgtgt cagcggttgg
     2521 ctgaacctgg caagccgggt gtctgcgcta tcgctaacgg cgtatgacgg ctgcaccggg
     2581 atattggtcg gcaagctgct gcaggccgcc atacccaggc aagccgcgat tgtcagtgtc
     2641 cgaatcagct tcacttgacg gcacactcca aataaatgtt ggtgctcacg cgcagaatgc
     2701 cgctgttgcc gtcagccggc agataacgcg tgtcgttttt cagtttcgat agcggccgtg
     2761 acttccagcc aactgcgggg gcgcgcaacc atgaacattt ggacatgatt atccttggcc
     2821 gttgaggatg gatcgaaaca agctagttgt gaaccgcagc gtggtcaagc cccggcaaaa
     2881 agcctttacc cgcgacgcag cgggtcctga tgcacaatgc agtggctacc gggcacccac
     2941 cctgcacgca cccgcaggtc gatgacagca agcgattcaa ggcgcttccg ccaccacaat
     3001 cgcccgcacc ggcgccggat aaccctcaat ggttttactg ctgtcattcg gatcaagaaa
     3061 atccggcagc gactggaacc gcatccagtc agtcgggcgc tgttcctgca cggtagtcgg
     3121 cgtcacatcc acgcaacgca cgttcctgaa ccgcactttc ttcagccagg ccagcagcgt
     3181 gggcacgctc ggcaggaacc acacgttgcc catccggcca tagcgccctt ccggcaccag
     3241 actgaatcct tcggggcctt ccacaatcag ggtttccagc accagctcgc caccgggacg
     3301 caacagttcg cgcaattcca gcagatgatc cagcggcgaa cggcgatgat agagcacacc
     3361 catggaaaac accgtgtcga acgcatgcag gtccggtggc agatgctcga tgcccagcgg
     3421 cagatgaaac accggctgcg gaccggtgaa atgttcgatg gcgcggaact ggcgcgtgaa
     3481 cagcagcgac ggatcgatgc cgatggtgag cgccgcgccc tccccgagca tgcgccacag
     3541 gtgatagcca ctgccgcaac cgatatcgag caccgtgcga ttttgcagtg actggatgtg
     3601 cggttgcagc cgttgccatt tccagtcgga acgccattcg gtgtcgatct ggatgccgca
     3661 gacattgaac ggacccttgc gccagggatg caggccgcgc aacgcggttt cgattttttc
     3721 ccgtgtagcg tcgtcgcagt cgcccggcac accgaccgtc acagagtctg cagcaaagtc
     3781 gcactgactc acctttaccg gtggcaaggc ttccacacaa cgttgccagc gcgcgaagtc
     3841 tccgtgctgg tcgggcacaa attgctgctg cagcgtgtgc atgaaggcat cggtccaggg
     3901 cgccagcgcg gaacgacgca tcgtctcggc aaaacgcgcg aactcctgca ttggatcaaa
     3961 agaagatggc gtgctgttcg cgccggctgg atcaatcata ctcatgcggc gctctcactt
     4021 cactgccaga aacgaggcga aactgaaaca ctggaaccag cagtgcactt gcgagaagcc
     4081 gttgcgcagc agacgctggc gatgctgctc cgccgtgtcc ggaatcagca cattttcaat
     4141 cgcctgccgc ttctggctga tttccagatc gctgtaacca ttaagacgtt tgaattcgtg
     4201 atgcagatcc gtgaagaggc tttgctgttc aaccgcgtca aaacagattt tttccgacag
     4261 caccagcaca ccgccaggat tcaggccgcg cgccactttt ccgatcatgg cgtcgcgctg
     4321 ttccggcggc aggaattgca gggtgaaatt cagcgccacc agagaagcgt tttccagcgg
     4381 ggtatcctgg atatcctcgc agcgcagctc cacccgggat ggcgacaggt ccgccgcgat
     4441 attgtgctgg cagcgctcga tcatggcaga cgaattgtcg acgcccacca gcgtgacatc
     4501 cggcacctgg atgtggcgac gcagggccag cgtagcggcg ccttgcgaac aacccagatc
     4561 gtaaacgcgg gaatggggcg tcacgtaatg actggcaaag acaccgagca tggcgatcac
     4621 ggtgtcgtaa ccaggcaccg agcggcggat catgtcgggg aaaacccgcg ccacctgctc
     4681 atcgaaggcg aaatccacca gatggggctt ggggcgggcg aacaggttgt ccttgtcgga
     4741 ggagtcggtc atggcggtca gcagcacgaa aaagtcgggg gcattctatg cccccgttgc
     4801 gatactgcca agcaggccgc cgccgcaacg gccggtgcgt ataaaaagcc gagtccgcac
     4861 aagcagttgc gcttcagcac cggatcagtt gcgctcggcc gacagcagca gggtatcgaa
     4921 gtggttggca aggtgaatca gctgctcttc ctccagcttc ttgaatacct tgttcaccac
     4981 gccgtgaccg gcagccctgc aggtggcaat ggcgccatcg gcgacctttt tgacgatgaa
     5041 attcagtggc aacgcggcaa tggagcgctt catcaactgc tccacctgga tgtccaccac
     5101 cagggacatc agatcgtgca gctccggcaa cacctgctga ccattgccgg cgcggacttc
     5161 ggcgatcacg cgcacaatgc ggtcataggt tggctggtcg atctcgcagc cagtggacgc
     5221 tttgccagta ctggcaatct cggcgcggac aaaaagttca tcgacaaact tgatgagtgg
     5281 tttcagctct tcattgctac gctttttcaa cagctgaccc accagcatct tggcagtttt
     5341 gtcgatgatg tccacggtgg aatgcacgat cttggtggcc atgggactga gccccactgc
     5401 gtccacc
//
LOCUS       DASUEG010000600         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1658578, whole genome shotgun sequence.
ACCESSION   DASUEG010000600 DASUEG010000000
VERSION     DASUEG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658578"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="VFX11_11275"
     CDS             <1..1805
                     /locus_tag="VFX11_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4939249.1"
                     /translation="DATISAVNEIALAVIATTFTLVAVFLPTAVMSGIAGLVFKQFGW
                     TVVTAVIASLLVARLVTPMMAIWILKAGHREPPEGVLMRTYLRSLQWCLRHRKTTVLA
                     SILFFAGSLSMIPLMPTSFIPAEDNGFVVINMELPPGSNLEASLATADEITRVLEGIP
                     GIETVFTSIGNKPVLPNVLSGPGEPRKGSISLILTSEDRPSQQEIERMIRPRLQSIAG
                     ARLSIGAGFTGEKMEILLVSQNIAALKNTAHNVERELRALPHFSGVQSTAALEQAEVT
                     IRPNAALAAERGVSTQAIADTVRIALAGDFDIALSKLNLDNRQLDIRVQVPVELRQNL
                     EAIGNLRVPGRYGLVPLASIAELSVESGPSQINRFNRQRQIAITIDLGGYPLGAAAKA
                     RDSLHAVKTMPPSVKLVDAGDAEFLAEMVESFGTALVSGVLLIYAVLVLLFKDWFQPI
                     TILSALPLSIGGAFIALLVGNFGLSLPAFIGLIMLMGIVTKNSILLVDFAVIAQRDLG
                     LSMHDALIDACRKRARPILMTTVAMIAGLLPLALGIGGDSSFRTPMAAAVIGGLVTST
                     ALSLLVVPVVFVYISRLQQWLTFRGKKHETQLQH"
     gene            1783..2448
                     /locus_tag="VFX11_11280"
     CDS             1783..2448
                     /locus_tag="VFX11_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939250.1"
                     /translation="MKRSSSTEDLAPAGENPGTRSVRQRIVDAALELAQTVGVQGMSQ
                     ARVAAAAGVRQSHLTYYFPTRADLIKATVYAISDQMLEVTKAALSATGLDIDPIQEMR
                     NFCMKEVCDLGKARLLLSLMVVAEEEPSLQQWNDEFHRETIGQWLEVYRNLGFDVNEA
                     DIELFHATFVGATLLGAQCGSEDAWQRAARVAGQAFDRLLQTRKPVPLRKARSARSPG
                     TKT"
     gene            complement(2465..2896)
                     /locus_tag="VFX11_11285"
     CDS             complement(2465..2896)
                     /locus_tag="VFX11_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939251.1"
                     /translation="MKRALVTFCLATALAFPVVASAEQQTIDPAATQKQLEEIHQHLM
                     NMEATIKKMESATSEAERKQLLAEHSALMDKGMALLGEVHVMDQVQRRGCTFHQKQNP
                     TMTCKVPQTTQGAEIDLMQMLMRMMYQQRAYDITVSKGLEH"
     gene            complement(3321..>4368)
                     /locus_tag="VFX11_11290"
     CDS             complement(3321..>4368)
                     /locus_tag="VFX11_11290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX4939252.1"
                     /translation="LNLRSWQDDGHKYRGIVVLLPESPGTKTRFTVAIAVDEQQHIAF
                     MAMFKLQLMLVGLAGLVLMGCLGWIVTRRGLMPVSTMARVAEGISAQRLDDRLQLDLL
                     PSELHSLAISFNDMLDRLEASLHRLSDYASDIAHELRTPVNTLMTQTQVSLSQSRTAD
                     QYREILYSNLEEYERLARIISDMLFLAKADNGLMIPSRQQVNVRDEVNALFEFYDAVA
                     AEKQVRLQTTGTAELRGDPLMIRRALSNLISNAIRHGAVGSAIDVNISSESGDVRISI
                     TNEGDTITPAQLERVFDRFYRADASRSRGDDGAGLGLGLAITRSIIEAHHGRVSATSA
                     YGRTCFVVVIPVNA"
BASE COUNT          916 a         1285 c         1241 g          926 t
ORIGIN      
        1 gcgatgccac catttccgcg gtcaacgaaa ttgcactggc cgtcatagcg acaaccttca
       61 cactggtggc tgtgttcctg cccacggccg tcatgagcgg tatcgccggg ctggtgttca
      121 agcagttcgg ctggaccgtt gtgacagcgg tcatcgcgtc gctgctggtg gcacgtctgg
      181 taacgcccat gatggcaatc tggattttga aggccggcca ccgcgaaccg ccggaaggcg
      241 tgctgatgcg tacctacctg cgcagcctgc aatggtgtct gcggcatcgc aagaccaccg
      301 tgctcgccag catcctgttt tttgcaggct cgctgtcgat gattccgttg atgcccacct
      361 cgttcattcc ggcagaggac aacggctttg tcgtcattaa catggaattg ccgcctggca
      421 gcaatctgga ggcgagcctg gccaccgccg acgagattac ccgggtgctg gagggcattc
      481 ccggcatcga aacggtattt accagcatcg gcaacaaacc ggtgctgccc aacgtgctca
      541 gtggccccgg cgagccgcgc aaaggctcca tcagcctgat actgacctcg gaagaccgtc
      601 cttcccaaca ggaaatcgaa cgcatgatcc ggccacggct gcagtcgatt gccggcgccc
      661 gcctttccat tggcgcgggt ttcactggag agaaaatgga gattctgctg gtcagccaga
      721 atatcgccgc actgaaaaac accgcccata atgtcgaacg cgaactgcgc gccctgcccc
      781 acttcagcgg cgtccagtcc acggccgccc tggaacaggc cgaagtcacc atccgtccca
      841 acgctgccct ggccgcggaa cggggcgtca gcacccaggc cattgccgac accgtgcgga
      901 tcgcgctggc cggtgatttc gatatcgccc tgtccaagct gaatctggac aatcgccagc
      961 tggatatccg ggttcaggta ccggtggaac tgcgccagaa cctggaagcc atcggcaatc
     1021 tacgcgtacc gggccgctac ggtctggtgc ccctggccag tatcgccgaa ctgtccgtgg
     1081 aaagcgggcc atcccagatc aaccgcttca accgccagcg ccagatcgcc atcaccatcg
     1141 acctgggcgg ctatccgctg ggcgcagctg ccaaggcgcg cgattcactg cacgccgtta
     1201 aaaccatgcc gcccagcgta aaactggtgg atgccggcga tgccgagttc ctggcagaaa
     1261 tggtggaaag tttcggtacc gctctggtgt ccggcgtatt gctgatctat gccgtgctgg
     1321 tgctgctgtt caaggactgg tttcagccga tcaccattct gtcggcgctg cccctgtcca
     1381 ttggcggagc attcatcgcc ttgctggtgg gcaactttgg cctgagcctg cccgccttca
     1441 tcgggctgat catgctgatg ggcatcgtga caaagaactc gatactgctg gtggactttg
     1501 cggtgattgc ccagcgcgat ctgggtctga gcatgcacga tgcattgatt gatgcctgcc
     1561 gcaaacgtgc acggccgatc ctgatgacca ccgtggccat gatcgcaggc ctgctgccgc
     1621 tggcgctggg tatcggaggg gattcctcgt tccgcacacc aatggcggct gcagtaatcg
     1681 gtggactggt gacgtccacc gcactgagcc tgctggtggt gcccgtggtg tttgtgtaca
     1741 tatcccgcct gcaacaatgg ctgacgttcc ggggaaaaaa gcatgaaacg cagctccagc
     1801 actgaagacc tcgcgccggc cggcgaaaat ccgggaacac ggtccgtgcg gcagcgcatc
     1861 gtagatgccg cactggaact ggcgcagact gttggcgtgc agggaatgag tcaggcccgt
     1921 gtggctgccg ccgctggcgt tcgccaaagc catctgacct actacttccc cacccgcgcc
     1981 gacctgatca aggccacggt ctatgccatc agcgatcaga tgctggaagt gaccaaggct
     2041 gctctgagcg ccacgggctt ggatatcgac ccgattcagg aaatgcgcaa tttctgcatg
     2101 aaagaagtgt gcgatctggg caaggcgcgg ctgctgctgt cgttgatggt ggtagccgag
     2161 gaagaaccgt cgctgcagca gtggaacgat gaattccatc gcgaaaccat cggccagtgg
     2221 ctggaggtgt accgcaatct gggctttgac gtgaacgagg cggatatcga gctgtttcac
     2281 gccacgttcg ttggtgccac gttgctgggg gcacagtgcg gctcggaaga tgcctggcag
     2341 cgagcggccc gcgttgccgg gcaggcattt gaccggttgc tgcagacccg gaagccggtg
     2401 ccgctacgca aggcacgcag tgcgcgttca cctggaacaa aaacgtagcg gcccggtttc
     2461 agcgttagtg ttcaagccct ttgctgacgg tgatgtcata cgcccgctgc tggtacatca
     2521 tccgcatcag catttgcatc agatctattt cggcaccctg tgtggtttgt ggcactttgc
     2581 acgtcatggt tggattctgt ttctgatgaa acgtgcagcc ccggcgctgc acctgatcca
     2641 tcacatgtac ctcgcccaat aacgccattc ccttgtccat cagggcactg tgttccgcca
     2701 acagttgttt gcgttccgct tcgctggtgg cactttccat ttttttgatg gtggcttcca
     2761 tgttcatcag atgttggtgt atttcttcca actgcttctg ggttgcggcg ggatcgattg
     2821 tttgttgctc agcacttgca acaacaggga atgccagagc agtagcaaga caaaaagtga
     2881 cgagagctct tttcatgggt cgtactcctt tacgtaggat tgcgcaggaa gttgcctgat
     2941 ccaatctacg cacgttcaat atgtaaatca cattacaaat atgtaatctg gcagatggat
     3001 tgcgttaagt tgacctgcgt cagaaatgca ggccgtagtg actcagcaaa taacgcgcaa
     3061 ctgtcgtcag atgtgtcggc tccacaacag caatcagtgg atcagcgtga aaagccactt
     3121 cgatgacgct ccccgcctct acgccccctg caaacctggc cgaaaaacgt aatccacctt
     3181 tgtgtcttac gcgttgtgca agcaacgctt ggataataat ttaccggttt ttccgcttta
     3241 agggcgccgc tggaatcata gagggcgaca tcgcttgccg gagcaatctg acaatccccg
     3301 ctaccaccgg atgcgcctgg ctaggcgtta acgggaatga ccacgacaaa gcatgtgcgg
     3361 ccgtacgccg atgtggcgct gactctgccg tggtgggctt cgatgattga acgtgtaatg
     3421 gccagcccca accccaaccc agctccatcg tcaccgcgcg agcgcgaggc atcagcacga
     3481 taaaagcggt cgaagacgcg ttccagctgt gcgggagtaa tcgtgtcgcc ttcattggtg
     3541 atgctgatac gcacatcacc cgactcagac gagatattca cgtcgatcgc agaccccacc
     3601 gcgccatgcc gaattgcgtt ggaaatcagg ttgctcagcg cacgccggat catcagcgga
     3661 tcacctcgca attccgctgt tccagttgtt tgcaaccgaa cctgcttttc cgccgcgacc
     3721 gcatcataaa attcaaatag agcgttaacc tcgtcgcgaa cgttgacctg ttggcgactt
     3781 ggaatcatca ggccgttgtc tgccttggcc aggaacagca tgtcagaaat gatccgtgcc
     3841 aatcgctcgt attcttcgag attggaatag agaatttcgc ggtactgatc tgccgtacgg
     3901 gactgggata gcgacacctg ggtctgcgtc atcagggtat tgaccggcgt cctcagctca
     3961 tgggcaatat ccgacgcata atcagacagg cgatgcagcg aggcttccag ccggtcgagc
     4021 atgtcgttaa aggaaattgc caacgagtgc aactcgctcg gcaacaagtc cagctgtaaa
     4081 cggtcatcaa ggcgttgggc cgaaatcccc tcggcgacac gcgccatggt actcaccggc
     4141 atcaagccgc ggcgagtgac aatccagcca agacaaccca tcaggacaag acccgccagc
     4201 ccaaccagca tgagctgcaa tttgaacata gccataaacg caatgtgttg ttgttcatcc
     4261 acagcgatgg caaccgtgaa gcgggttttt gtaccgggcg attccggcag aaggacaaca
     4321 atcccacgat acttgtgccc atcatcctgc caggaacgga gattcaag
//
LOCUS       DASUEG010000601         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_684439, whole genome shotgun sequence.
ACCESSION   DASUEG010000601 DASUEG010000000
VERSION     DASUEG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684439"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFX11_11295"
     CDS             complement(<1..677)
                     /locus_tag="VFX11_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX4939253.1"
                     /translation="MHEIPLSFLWTALAILLTMSAFFSSSETGMLSLNRYRLRHLVKK
                     KQRGAALAEELLARPDRLIGMILIGNNFVNTFAASIATIIAIRLWGENSIALASAVLT
                     LAVLVVGEVTPKTLAALRPEKVAFVAVYALKPLMTLLYPLVWLMNTVCNGLLRAIGVT
                     PEEAGSDHLSREELRTLVNESGAILPKRHKSMLLSILDLEDVTVDDIMIPKHEVMGID
                     LEEDIDE"
     gene            complement(693..1499)
                     /gene="ccsA"
                     /locus_tag="VFX11_11300"
     CDS             complement(693..1499)
                     /gene="ccsA"
                     /locus_tag="VFX11_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX4939254.1"
                     /translation="MTPAIAAGIFACLCYGFAAFLLVRSLKTNTPLNRPFLLGVAVLG
                     LAAHAFNLNAALFRHDGIEFGLINVISLFGFVLAACGTLITLFRDIQSLLAPAYPAAC
                     IGVLLNLFMHDRVGPHIDLNGGMVAHILLSVVAYSVIALALSQAMLLSIQNYQLKHRH
                     IHDILHLLPPLQTMENTLFDLISIGLLLLTAAIGSGLLFVEDFFAQHLLHKTVFALGA
                     WLVLWMMIVGRHLWGWRGMVAVRWTLAGFLLLTLAYFGSKFVLEVVLQRV"
     gene            1619..>2433
                     /locus_tag="VFX11_11305"
     CDS             1619..>2433
                     /locus_tag="VFX11_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011704629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX4939255.1"
                     /translation="MFDTLTERLSNSLRSVTGKARLTEDNIKDTLREVRMALLEADVA
                     LPVVKDFIDKVKERAIGQEVMKSLSPGQVFIKIVNEELVHMMGDANDALKLNTQPPAI
                     ILMAGLQGAGKTTTVGKLAKFLKERHKKTVLVTSADVYRPAAIKQLETLAHDIGVKFH
                     PSSADQNPIDIARAAIADAKKQFADVVIVDTAGRLAIDEQMMDEIKQLHAAINPIETL
                     FVVDAMTGQDAANTAKAFNDALPLTGVVLTKADGDARGGAALSVRHITGKPIK"
BASE COUNT          574 a          752 c          707 g          400 t
ORIGIN      
        1 atctcatcga tgtcctcctc cagatcgatg cccatcactt catgcttggg aatcatgatg
       61 tcatcgacag tgacatcctc cagatccaga atgctcagca acatggattt gtgacgcttt
      121 ggcagaatgg cgccggactc attcaccaaa gtgcgcagct cctcgcggct gaggtgatcg
      181 gagccggctt cctccggcgt cacaccaatg gcgcgcagca ggccgttgca cacggtattc
      241 atcagccaca ccagcggata cagcagcgtc atcagcggct tcagcgcata caccgccaca
      301 aatgccactt tctcgggtcg cagggccgcc agcgttttgg gtgtgacttc gcccaccacc
      361 agcacggcca gtgtcagcac ggcgctggcc agcgcaatgc tgttctcacc ccacaggcgg
      421 atagcgatga tggtggcgat ggacgcggcg aaagtattca cgaagttgtt gccgatcagg
      481 atcatgccga tcaaccggtc ggggcgggcc agcagttctt cggccagtgc agcgccgcgc
      541 tgctttttct tcaccagatg acgcaggcgg taccggttga gtgacagcat gccggtttcg
      601 gaactggaga agaacgccga catcgtcagc aggatggcca gcgcggtcca gagaaaggag
      661 aggggaatct catgcacggc ggcgcgacct gttcataccc gctgcagcac cacttccagc
      721 acaaatttgg aaccgaaata cgccagtgtc agcaacagga aaccggccag ggtccagcgc
      781 accgccacca tgccgcgcca gccccacaga tggcggccca cgatcatcat ccacagcacc
      841 agccaggcgc ccagcgcgaa cacggtcttg tgcagcaggt gctgggcgaa aaaatcctcc
      901 acgaacagca ggccgctgcc gatggcggcg gtgagcagca acagcccgat cgagatcagg
      961 tcgaacaggg tgttttccat cgtctgcaac ggcggcagca gatgcaggat gtcgtggata
     1021 tggcggtgct tgagctggta attctggatc gacagcagca tcgcctgcga cagggccagt
     1081 gcaatcacgc tgtaggccac caccgacagc aggatgtgcg ccaccatgcc gccattcagg
     1141 tcgatatgcg ggcccacccg gtcatgcatg aacaggttca gcagcacgcc gatacaggcg
     1201 gcgggatagg ccggtgccag cagggattga atatcgcgga acagggtgat cagggtgcca
     1261 caggcggcga gcacgaaacc gaacagggaa attacattga tcaggccgaa ttcaatgccg
     1321 tcgtgacgaa acagcgcagc attcagattg aaggcatggg ccgccagacc cagcaccgca
     1381 acgcccagca gaaatggccg gttgagcgga gtgttggtct tcagactgcg aaccagcagg
     1441 aaggcggcga aaccataaca gagacaggcg aaaattcctg ctgcaatcgc cggagtcatg
     1501 gcgctggtgg gctccttact ggattcgggg aaggacaagt gtcgcacaag gctgtcaata
     1561 aggaaacggc ctgcgctata cttccgcgct cgataacaat acccacgaag atgcccccat
     1621 gtttgacaca ctgaccgaac gcctcagtaa cagcctgcgc agcgtcaccg gcaaagcccg
     1681 tctcaccgaa gacaacatca aggacaccct gcgcgaagtg cgcatggccc tgctggaagc
     1741 ggatgtggca ctgccggtcg tgaaggactt tatcgacaag gtgaaagagc gggccattgg
     1801 tcaggaagtc atgaagagcc tgagccccgg ccaggtcttc atcaagattg tcaacgaaga
     1861 gctcgtccac atgatgggcg acgccaacga cgccctcaag ctcaacaccc agccacccgc
     1921 cattatcctg atggccggcc tgcagggtgc cggtaaaacc accaccgtgg gcaagctggc
     1981 caaattcctg aaagagcggc acaagaaaac cgtgctggtc accagcgccg acgtttaccg
     2041 tcctgccgcg atcaagcagc tggaaaccct ggcccacgat atcggcgtga agttccaccc
     2101 cagcagtgct gaccagaatc ccatcgacat cgcccgcgcc gctatcgctg acgccaaaaa
     2161 gcagtttgcc gacgtggtaa tcgtggacac cgccggccgt cttgccatcg acgaacagat
     2221 gatggacgag atcaagcagc tgcacgcggc gatcaatccc attgaaaccc tgttcgtggt
     2281 cgacgccatg accggtcagg atgccgccaa cactgccaag gcattcaacg acgccctgcc
     2341 gctcactggc gtggtcctca ccaaggccga cggtgatgcc cgtggcggtg ccgcgctctc
     2401 ggtgcgccac atcaccggca agccgatcaa att
//
LOCUS       DASUEG010000602         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1395483, whole genome shotgun sequence.
ACCESSION   DASUEG010000602 DASUEG010000000
VERSION     DASUEG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395483"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="pyrB"
                     /locus_tag="VFX11_11310"
     CDS             <1..527
                     /gene="pyrB"
                     /locus_tag="VFX11_11310"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787164.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4939256.1"
                     /translation="TIRRHKGAFENLTVAIVGDVLHSRVARSQIHALNALGAREIRVI
                     APKTLVPKDIEAMGVKVHYNMAQGLQDVDVISMLRLQKERMESALLPSESEFYRLYGL
                     NMEKLAFAKPDAIVMHPGPINRGVEIESAVADGAQSVILQQVNYGIAVRMAVLSMAMS
                     GQNAEQAAQAEGGE"
     gene            527..1828
                     /locus_tag="VFX11_11315"
     CDS             527..1828
                     /locus_tag="VFX11_11315"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4939257.1"
                     /translation="MDFSRPAFNQRIRISNGRVIDPRNGLDKTTDLFVADGLIVGIGS
                     APVGFKPDLEFDAAGMLVIPGAVDLCARVREPGQTQKGNIHSETRAAAAGGITHLCCP
                     PDTKPVIDTRAVAALIQERAYQSGYAHVLPIGALTKGLQGQHLAEMHALKEAGCIGVS
                     QLRQPVKDSQTLLRCLEYASTFDLIVFFQSEDSSLSMKGGVHAGSTASRLGLHGIPDA
                     AETVALSRDLLLVQQAGVRAHFGQLSCARSVELIAAAQREGLQVTADVAVHQLFLTDE
                     MIDGFNSQYHTRPPLRSERDRVGLIEGVKSGVISVLSSDHQPHEAAAKSEPFPSTEPG
                     ISAFATFLPLSYRLVREGVLSEVEWVRRIATNPAALTGLDAGHLGTGSNANLCVFDPG
                     KRWKLTEHSMLSQGKNSPFLGQSMQGQVRLTLKDGAISFRN"
     gene            complement(1812..3743)
                     /locus_tag="VFX11_11320"
     CDS             complement(1812..3743)
                     /locus_tag="VFX11_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939258.1"
                     /translation="METQVQIPVAATGILRRMTLSYGAGALVVLLVALFTHLPTSFGQ
                     FSTDDFLIRAMTAGDPVLFEKGFAKADPDKPVWRSVLDGFHFYTPGGGTLATYREYGN
                     LPWWTAEAATMNPLRPVTAFTHWIDMQIAPDSFAFQSFHSLLYILLFAWSGYRLFWRI
                     SPVASVAVLASLLLVVDFSHLLNYKWVAARNVFIAGALGCAALERFLAWRQQGSPVAM
                     AASILLFTIGLLSAEATVAVGGYLVAYLLLVERSGLAKAFLLLLPYGVVVILWRLTYN
                     QLGFGADGIGLYVDPGRSLLDFFKTSLQILPLMLASVITSMDGFATSATSDARLWLSL
                     GSAVLALGCVALVLPLLRQNPWVRFMLAGSLLAAVPGSALISAGSRSGLFLAIGFFWV
                     MAMWLHWLARNPGQRFNRVVFGATLCLHLFFPAFAGFVYTSQLLPVGYFDDEQFESVA
                     KPLQAADGHRALVIVNSPIPTTAFYLPFEWRFKYGVAPDSMKMLAPGLVTFDLKRKSA
                     REFELIAPAGLPLNHTSDVRDLQGNRQNFSSVYAALLLQGLFTSPETRMQPGFRQQSA
                     DMQVTVLELVEGRPSRLRIEFTGTENPDEMLWQRYDWKEREYRALPVPAIGETRHFAG
                     PFDTDADLNVVKLCVNCEN"
     gene            complement(4036..>4955)
                     /locus_tag="VFX11_11325"
     CDS             complement(4036..>4955)
                     /locus_tag="VFX11_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX4939259.1"
                     /translation="ARGIGRFRVSAFYQRNLVGMVLRRIETRIPTIDELGLPSVVKDL
                     AMTKRGIVIFVGATGTGKSTSLASMIGHRNINSKGHIISIEDPIEYIHQHKGCIVTQR
                     EVGLDTESFDVALKNTLRQAPDVIMIGEIRTRETMEYAVAFSETGHLVLATLHANNAN
                     QALDRIIHFFPADRHGQLWMDLSLNLRSIVAQQLIPTPDGKSRRAVIEVLLNTPLMAD
                     LIRKGEVHLLKPLMAKSRELGMQTFDQALYDLYSKGEITYEDALSHADSPNDLRLMIK
                     LGSESTPDSLADATKGLTLQDDDSLRSRW"
BASE COUNT          995 a         1445 c         1600 g          915 t
ORIGIN      
        1 acaccatccg ccgccacaaa ggggcgtttg aaaatctcac cgtggcgatt gtcggcgacg
       61 tgctgcattc gcgggtggcg cgctcgcaga ttcacgcgct gaatgcgctg ggcgcgcggg
      121 aaatccgtgt cattgccccg aaaacgctgg tgccgaaaga catcgaagcc atgggtgtga
      181 aagtgcacta caacatggcg caggggctgc aggatgtgga tgtgatttcc atgctgcggc
      241 tgcagaaaga acgcatggaa tctgcgttgc tgccgagcga atccgaattt taccgtctgt
      301 atggtctcaa catggaaaaa ctggcgtttg ccaaaccgga tgcaatcgtg atgcaccccg
      361 gccccatcaa tcgcggcgtg gaaatcgagt ctgccgtggc cgacggcgcg cagtcggtga
      421 ttctgcagca ggtgaattac ggcattgcgg tgcgcatggc ggtgctgtcg atggccatga
      481 gcgggcagaa cgccgaacag gctgcacaag cggaaggggg tgagtgatgg atttttcccg
      541 ccccgcgttc aaccagcgca tccgcatcag caatggccgc gtgatcgatc cgcgcaatgg
      601 tctggataaa accaccgatc tgtttgtggc ggacggcctc atcgtcggca tcggcagtgc
      661 gccggtgggc ttcaagccgg atctggaatt cgacgctgcc ggcatgctgg tgattcccgg
      721 cgcggtggac ctgtgcgccc gcgtgcgcga acccggccag acgcagaaag gcaacattca
      781 cagcgaaacc cgcgcggcgg cggctggcgg catcacgcac ctgtgctgcc cgcccgacac
      841 caaaccggtg atcgacaccc gtgcggtagc cgcgctgatt caggagcggg cctatcaatc
      901 aggctatgcc cacgtgttgc ccatcggcgc gctgaccaag ggtttgcagg gccagcatct
      961 ggcagaaatg catgcgctga aggaagcagg ttgcattggc gtgtcgcagt tgcgccagcc
     1021 ggtgaaggac agccagacgt tgctgcgttg tctggaatac gccagcacct tcgatctgat
     1081 cgtgtttttc cagagcgagg acagttcgct gagcatgaaa ggcggtgtgc atgccggttc
     1141 caccgcgagc cggctgggat tgcacggtat ccccgatgcg gccgaaacgg tggcgctgag
     1201 ccgcgatttg ttgctggtgc agcaggccgg cgtgcgggcc cacttcggtc agctgtcctg
     1261 cgcccgcagt gtggagctga ttgcggcggc acagcgggaa ggtttgcagg tgacggcgga
     1321 tgtggccgtg caccagctat tcctgaccga tgaaatgatc gacggtttca acagccagta
     1381 tcacacccgc ccgcccctgc gcagcgagcg tgaccgcgtg ggtttgatcg agggtgtgaa
     1441 atcgggtgtg atctcggtgc tcagttccga tcaccagccg catgaagcgg cggcgaaatc
     1501 cgaaccgttt ccgtctaccg agcctggcat ttccgcattc gcgacgtttc tgccgctgag
     1561 ttaccggctg gtgcgggagg gtgtgctgag cgaagtggaa tgggtgcggc ggattgccac
     1621 caacccggcg gcgctgaccg ggctggatgc gggccacctg ggcaccggca gcaatgctaa
     1681 cctgtgcgtg ttcgatccgg gcaagcgctg gaagctcaca gagcattcca tgctgtcgca
     1741 ggggaaaaat tccccgttcc tgggtcagtc gatgcagggt caggtgcgcc tgaccctgaa
     1801 agacggtgcc atcagttttc gcaattaacg cagagtttca ccacgttcag gtctgcgtcc
     1861 gtgtcgaacg gcccggcgaa atgccgggtc tcgccgatag ccggcaccgg cagcgcccgg
     1921 tattcgcgct ccttccagtc atagcgttgc cacagcatct cgtcgggatt ttctgttccg
     1981 gtgaattcaa tgcgcaatcg cgacggccgg ccttccacca gttccagcac ggttacctgc
     2041 atgtcggcgg attgctgacg gaagccgggt tgcatgcggg tttccggtga tgtgaacagc
     2101 ccctgcagca gcagggcagc gtagacggac gaaaaattct gacggttgcc ttgcagatcc
     2161 ctgacgtcac tggtgtgatt cagcggcagg cctgccggtg cgatcagttc gaattcccgc
     2221 gccgacttgc gtttcaggtc aaacgtcacc aggcccggtg ccagcatctt catggaatcc
     2281 ggtgccactc cgtacttgaa gcgccactcg aagggcaggt aaaacgcagt ggtgggaatc
     2341 ggtgaattga cgatgaccag tgcgcgatgg ccatcggccg cctgcagggg cttggccacg
     2401 ctttcaaact gttcgtcgtc gaaataaccc accggcagca gttgcgaggt gtagacgaat
     2461 ccagcgaacg ccgggaaaaa caggtgcaga cacagggtgg cgccgaatac tacgcggttg
     2521 aagcgctggc ccgggttccg cgccagccag tgcagccaca tcgccatgac ccagaaaaag
     2581 ccgatcgcca gaaacaggcc gctgcgcgac ccggcggaga tcagcgccga cccgggcacg
     2641 gccgccagca gtgatcccgc cagcatgaaa cgcacccagg gattctgccg cagcaggggc
     2701 aacaccagcg ccacgcatcc cagggcaagc accgcgctgc ccagggaaag ccatagccgc
     2761 gcgtccgatg tcgcggacgt ggcgaatcca tccatcgagg taatgacaga agccagcatc
     2821 agcggcagga tctgcaatga tgttttgaaa aaatccagca acgaacggcc cgggtccaca
     2881 tacaggccga taccatcggc gccaaaaccc agctggttat aggtcagccg ccacagaatc
     2941 acgaccacac catagggcag cagtaacagg aaggctttgg ccaggccgct gcgttccacc
     3001 agcaacagat acgccaccag atagccaccc acggccaccg tggcctcggc actcaacagc
     3061 ccgatcgtga acagcaggat ggacgcggcc atcgccaccg ggctgccttg ctgccgccag
     3121 gccagaaacc gttccagggc ggcgcaaccc agggcgccgg caatgaacac gttgcgggcc
     3181 gcgacccatt tgtagttgag caggtggctg aaatccacca ccagcagcag gctggccagt
     3241 acggccaccg acgcaaccgg cgagatgcgc cagaacaggc ggtagcccga ccaggcaaac
     3301 aacagtatgt acagcaggga atggaatgac tggaacgcaa acgaatccgg ggcgatctgc
     3361 atatcgatcc agtgcgtgaa cgccgttacc ggacgcagcg gattcatggt ggcggcctcg
     3421 gctgtccacc agggcaggtt gccgtattcg cgataggtgg ccagggtgcc gccgccgggt
     3481 gtgtaaaaat gaaagccatc cagcacgctg cgccagacgg gcttgtcggg gtctgccttg
     3541 gcaaagcctt tttcaaacag caccggatcg cctgctgtca tggcgcggat caggaagtca
     3601 tcggtcgaga actggccgaa cgatgtcggc aaatgcgtga acagggcgac gagcaggacg
     3661 accagtgctc ccgcaccata ggaaagggtc atgcggcgca ggatacctgt cgcggcgacc
     3721 ggaatttgga cttgagtttc cattgttgtt cttccctggt tgtggtcagc aaaaagcatg
     3781 cttcgacccc agtccccctc tgtggcgctg cattgagggc gtgatgctac cccctgaatt
     3841 gggacaaaag tactgtccag ggaggccatt ctgcagactc tgtgacgcca ttcaacccgt
     3901 tttcacaatc tgacaacaat gtaattcaaa attgcccttc tcaagttagg ggaatagtgc
     3961 caggagcgga tgatttccgg ccataaagca caaaacccgc cgaagcgggt tctgttttcg
     4021 gggcggacgc agtgtttacc agcgggagcg cagcgagtcg tcgtcctgca gggtgaggcc
     4081 cttggtggca tcggccaggg agtccggcgt ggattccgag cccagcttga tcatcaggcg
     4141 caggtcgttg ggcgaatcgg cgtgggacag ggcgtcctca tacgtgatct cgcccttgct
     4201 gtacaggtcg tacagggcct gatcgaaggt ctgcatgccc agttcgcggg acttggccat
     4261 cagcggtttc agcaggtgca cttcgccctt gcggatcagg tcggccatca gcggggtatt
     4321 gagcagtact tcgataacgg cgcggcggct cttgccgtcg ggggtgggaa tcagctgctg
     4381 ggcgacgatg gagcgcaggt tcagcgacag gtccatccac agctgcccgt gccggtccgc
     4441 cgggaagaag tgaatgatgc ggtcgagcgc ctggttggcg ttgttggcgt gcagagtcgc
     4501 cagcaccagg tgaccggttt ccgagaacgc cacggcatat tccatggttt cgcgggtacg
     4561 gatctcgccg atcatgatca cgtcgggtgc ctgccgcagg gtgtttttca gcgcgacgtc
     4621 gaaactttcg gtatccagcc ccacttcgcg ctgcgtgacg atgcagccct tgtgctggtg
     4681 gatgtattcg atcgggtctt cgatgctgat gatgtggccc ttggagttga tgttgcggtg
     4741 gccgatcatc gaggcgagtg aggtggattt accggtcccg gtggcgccca cgaaaatgac
     4801 gatgccgcgc ttggtcatgg cgaggtcttt caccacggac ggcaggccca gttcgtcgat
     4861 ggtgggaatg cgggtttcga tgcggcgcag caccatgccc accaggttgc gctggtagaa
     4921 ggcgctgacc cggaaccggc caatgccgcg ggcgc
//
LOCUS       DASUEG010000603         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_840310, whole genome shotgun sequence.
ACCESSION   DASUEG010000603 DASUEG010000000
VERSION     DASUEG010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840310"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFX11_11330"
     CDS             complement(<1..1147)
                     /locus_tag="VFX11_11330"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX4939260.1"
                     /translation="MPNTLLPWLIFIPLLAGVLSWLSGRIHAQAPRWVALAGMVAVAV
                     AVCSLWPTAPQTGWWSEFQAPWIPGWGISFHLALDGLSALMVLLAALLGVIAVLASWK
                     EVQSGVGLFHLHLSLMLAGVLGVFLAVDLFLFFVCWEIMLLPMYFLITLWGHEAQGGR
                     GRFHAATKFFIYGQASGLLMLLAIVGLAFANFAATRVVTFDYEMLLQTQLNPVVGFVL
                     MLGFFIAFAVKLPAVPFHGWLADAHQHAPTGASVDITGLLLKTSAYGILRFCLPLFPE
                     ASQQFAETAMIIGVASVIYGGVLAFGQTHLKRLVAYTSISHMGFILIGIYAFNAVALQ
                     GVVVMMVAGALSTAGLFVLCGQLYERLGTFDTREMGGLWTRLPTLPPI"
     gene            complement(1163..3010)
                     /gene="nuoL"
                     /locus_tag="VFX11_11335"
     CDS             complement(1163..3010)
                     /gene="nuoL"
                     /locus_tag="VFX11_11335"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX4939261.1"
                     /translation="MNLLYLTVLFPLLGYVILSWSRGRLSENLAAVVGVGSLFLSAFT
                     TAFVGYHFLTAPPEAGVFTQTLWTWINVDGFAPRVSFHLDGLALTMLGVITGVGALIH
                     LFASWYMRGEEGYSRFFSYMNLFVASMVVLVLADNLLLLYLGWEGVGLCSYLLIGFYY
                     KEKANGAAAQKAFIVTRIGDVFMALGLFMLYRETGTLDIPVLLDKAPELFTAGSVTIN
                     LIALMLLGGAVGKSAQMPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHPLFL
                     LAPGVLNLVGIIGAFTLLIAGFSALVQTDIKRILAYSTMSQIGYMFLALGVGAWQPAI
                     FHLMTHAFFKALLFLSSGAVILACHHEQNIFRMGGLYKQIPLVYVCFLVGGAALVALP
                     FVTVGFYSKDEILWETYASHHEWLLLCGWIGAFMTSLYTLRLIFVAFHGPAQIQAHGG
                     HGIAYGLPLVVLLVLSTGVGALIHPPLQDVLPLGAGHDGGEMKHWIELWSGAIAVAGV
                     VLAALLFAGERRMVKVVAANPAGRALSSLWYQAWGFDRLYDVVFTKPFLWLSNILASD
                     LLDRLTYLIAGFAKAVNLVAAAAENGQVRSYAASIAIGALLTLLILIAL"
     gene            complement(3007..>3230)
                     /gene="nuoK"
                     /locus_tag="VFX11_11340"
     CDS             complement(3007..>3230)
                     /gene="nuoK"
                     /locus_tag="VFX11_11340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX4939262.1"
                     /translation="ILFMLMSLEVMMNAAALAFVVAGSRWGQPDGQIMFIFILTLAAA
                     EASVGLAFVFQLYRRFQVVDIDAASEMRG"
BASE COUNT          739 a         1017 c         1020 g          454 t
ORIGIN      
        1 cgatcggcgg cagcgtgggc aggcgggtcc agaggccacc catctcgcgg gtgtcgaagg
       61 tgcccaggcg ctcatacagc tggccgcaca gcacgaacag gccggcggtg gacagcgcac
      121 ctgccaccat catcactacc acgccctgca gcgccacggc gttgaaggca taaatgccga
      181 tcaggatgaa gcccatgtgg gaaatcgagg tgtaggccac cagccgcttc agatgggtct
      241 ggccgaaagc gagcacgccg ccgtagatca cgctggccac gccgatgatc atggcggttt
      301 cggcgaactg ctgcgaggct tccgggaaca gcggcaggca gaagcgcagg atgccgtagg
      361 cggacgtttt cagcagcagg ccggtgatat ccacgctggc gccggtgggg gcatgctggt
      421 gcgcatccgc cagccagccg tggaaaggca ctgccggcag cttcaccgca aacgcgatga
      481 agaagcccag catcaggacg aagccgacca ccggattgag ctgggtctgc agcagcattt
      541 cataatcgaa ggtcaccacc cgggtggcgg cgaaattggc gaacgccagc ccgacgatgg
      601 ccagcagcat cagcagtccg ctggcctggc cgtaaatgaa aaacttggtg gcggcgtgaa
      661 agcggccgcg tccgccctgc gcttcgtggc cccacagggt gatcaggaaa tacatcggca
      721 gcagcatgat ttcccagcag acgaaaaaca ggaacagatc cactgccagg aacacgccga
      781 gcacgccggc cagcatcagc gacaggtgca ggtggaacag gccgacgccg ctttgcactt
      841 ccttccacga cgccagtacc gcaatcacac ccagcagcgc tgccagcagc accatcaggg
      901 ccgacaggcc atccagtgcc aggtgaaaac tgatgcccca gccgggaatc cagggcgcct
      961 ggaattcgct ccaccagccg gtttgcggtg cggtgggcca gagactgcag acggcgacag
     1021 ccacggcgac cattcccgcc agtgccaccc agcggggcgc ctgcgcatga atacgcccgc
     1081 tcagccagga cagcacgcct gccagcagag ggatgaaaat gagccagggt aaaagggtat
     1141 tgggcatgat gtcgttatcg cgttaaaggg caatcaaaat cagcaaggtc agcagcgcgc
     1201 cgatggcaat cgaagcggcg tacgagcgca cctgtccatt ttccgccgca gcggcgacca
     1261 gattcacggc cttggcaaag ccggcgatca gatacgtcag gcggtccagc aggtcggacg
     1321 ccagaatatt cgacagccac aggaacggct tggtaaatac cacatcgtac aggcggtcga
     1381 agccccaggc ctggtaccac aggctgctga gggcgcggcc cgcaggattg gcggcaacca
     1441 ctttcaccat gcggcgttcg ccggcaaaca gcagtgcggc cagtaccacg ccagccacgg
     1501 caatggcgcc ggaccagagt tcgatccagt gcttcatctc gccaccgtca tggcccgcac
     1561 ctaacggcag cacgtcctgc agcggcggat gaatcagcgc acccacaccg gtggacagca
     1621 ccagcaacac caccagcggc aggccgtagg caatgccgtg gccgccatgg gcctggatct
     1681 gggccgggcc gtggaaggcc acgaaaatca ggcgcagggt atacagcgac gtcatgaacg
     1741 caccgatcca gccgcacagc agcagccatt catggtgact ggcgtaggtt tcccacagga
     1801 tctcgtcctt gctgtagaag ccgacggtga cgaagggcag ggcaaccagt gcggcgccgc
     1861 ccaccaggaa gcacacgtac accagcggaa tctgcttgta caaaccgccc atcctgaaaa
     1921 tgttctgctc gtggtggcag gccaggatca ccgcgccgga cgacaggaac agcagcgcct
     1981 tgaagaaggc gtgggtcatc aggtggaaga tcgccggttg ccaggcgccc acacccaatg
     2041 ccaggaacat gtagccgatc tggctcatgg tggagtaggc cagaatgcgc ttgatgtcgg
     2101 tctgcaccag ggccgagaag ccggcgatca gcagggtgaa ggcgccgatg atgcccacca
     2161 ggttgaggac gccgggcgcc agcaggaaca gcggatgggt gcgggcgatc aggtagacgc
     2221 cggcggtgac catcgtggcc gcgtggatca gcgccgatac cggcgtggga ccggccatcg
     2281 cgtccgccag ccaggtctgc agcggcattt gcgcggattt gcccacggcg ccgcccagca
     2341 gcatcagggc aatcaggttg atggtcacgg agccggcggt gaacagttcc ggcgccttgt
     2401 ccagcaatac gggaatatcc agcgtgccgg tttcgcggta caacataaac aggcccaggg
     2461 ccatgaacac gtcgccaatc cgggtgacga taaaggcttt ctgcgccgcc gccccgttcg
     2521 ccttttcctt gtagtagaaa ccgatcagca gataactgca cagacccacg ccttcccagc
     2581 ccaggtacag cagcagcagg ttgtccgcca gcaccagcac caccatgctg gccacgaaca
     2641 ggttcatgta cgagaagaaa cggctgtagc cttcctcgcc gcgcatgtac caggaggcga
     2701 acaggtggat cagcgcgccg acgccggtga tcacgccgag catggtcagc gccaggccat
     2761 ccagatggaa cgacacgcgg ggcgcgaagc catcgacgtt gatccaggtc cacaacgttt
     2821 gcgtgaacac gccagcttcc ggcggggcgg tcaggaagtg gtaaccgacg aaggccgtgg
     2881 tgaaggcgga caggaacagc gagcccacgc caacgacggc agccagattt tcggacagcc
     2941 ggccacggga ccaggacagg atcacatagc ccagcagggg aaacaggacg gttaaataga
     3001 gcaggttcat ccgcgcatct cactggcagc gtcgatgtcc accacctgga accgacggta
     3061 aagctggaac acaaaagcca gacccacgct ggcttctgct gccgccaggg tcaaaatgaa
     3121 aatgaacatg atctggccgt cgggctggcc ccagcggctg ccggccacca cgaacgccag
     3181 cgctgcggca ttcatcatca cttccaggct catcagcatg aacaggatgt
//
LOCUS       DASUEG010000604         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2051779, whole genome shotgun sequence.
ACCESSION   DASUEG010000604 DASUEG010000000
VERSION     DASUEG010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051779"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFX11_11345"
     CDS             complement(<1..360)
                     /locus_tag="VFX11_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4939263.1"
                     /translation="MLEFLLYPLLGVFAGLSAGLLGVGGGIIVVPVLIYSFSALGFSS
                     EVLTHMAVGTSLATIIITSTGSVFQHHRKGSVLWSVLTWFTVGLVAGALLGATLADLL
                     HGRILQILFGIFALLIAA"
     gene            complement(365..1321)
                     /locus_tag="VFX11_11350"
     CDS             complement(365..1321)
                     /locus_tag="VFX11_11350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX4939264.1"
                     /translation="MNPNYLDFEQPIAELESKINELSGVTKDNAALNLTDEIKLLKEK
                     SHALTATIFSGLSSWQIAQLARHPKRPYTLDYVDRIFTEFDELHGDRTFGDDKAIIGG
                     TARLDGKPVVIIGHQKGRDVKEKVRRNFGMPQPEGYRKAMRLMQLAERFKLPVLTFID
                     TPGAYPGIQAEERGQSEAIARNLAVMASLRTPLIATVIGEGGSGGALAIGVCDHLQML
                     QFSTYSVISPEGCASILWKSAEFAADAAEAMGLTAARLHELGIVDQVIPEPLGGSHRD
                     HDTTAQRLKAALVENLDRLQKMPEDALLNRRYDRLMSYGFSG"
     gene            complement(1460..4927)
                     /gene="dnaE"
                     /locus_tag="VFX11_11355"
     CDS             complement(1460..4927)
                     /gene="dnaE"
                     /locus_tag="VFX11_11355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX4939265.1"
                     /translation="MTPQFVHLRMHTEYSLVDGLVRVKPLAKTAAALGMPAIAITDYS
                     NMFALVKFQKAATEAGIKPIYGADVWLEAAQTDEPPSQIVLLAKNLQGYRNLTVLVSK
                     GYLFGQQRNRVIISRDWLKQKSDGLICLSGGKSGEIGRALLGGKLDRAELLTQEMMDL
                     FPDSFYLELQRTQRKDDETYLHLAVDLAAKTDCPVVATNDVRFLKRSDFEAHEVRVCI
                     SEGWTLEDPSREQRYSEEQYLKPAAEMVELFADIPEAIENTVEIAKRCSVNIPLGTYY
                     LPEYPVPDGMTIADFMVVESRKGLEERLAFLFDVNDPTFSDIRKPYDERLQVELDCIV
                     KMGFSGYFLIVADFIQWAKNNGVPVGPGRGSGAGSLVAYALKITDLDPLEYDLLFERF
                     LNPERVSMPDFDVDFCMDGRDRVIDYVAEKYGRNAVSQIITFGSMAAKAVVRDVGRVQ
                     GRSYGFVDRIAKLIPFEVGMTLSKAMEQEPQLKELYDRDDDIRELLDMALKLEGITRN
                     VGKHAGGVVIAPSALTDYVPLYCDAEGGNLVTQFDKDDVEAAGLVKFDFLGLRTLTIV
                     DWALKTINPVLEKRGEKPVEIERIPLDDRATFELLQRAETTAVFQLESRGMKDLIKRL
                     KPSNFEDIVALVALFRPGPLGSGMVDDFIARKHGKQKVVYPHPDLQPILNTTYGTILY
                     QEQVMLIPQVLANFTLGGADLLRRAMGKKKADEMAKQRAFFIDGAAKNSVDEKLAGEI
                     FDTMEEFAKYGFNKSHSAAYALVSYQTAWLKTHHPAGFMAAVLSADMHNTDKVVTLID
                     ECRRMKLVIVPPDVSYSQYKFTVDERGRIIYGLGAIKGLGEGPIDAILEARNSGKPFK
                     DLFEFCRRVDMKKLNRRALEALIRAGALDELGPDRATLMASLNEATKMAEQASKNQDI
                     GIVDLFGAIEEETAAEPEWAKVKPWSDDERLNGEKDTLGLYLTGHPIDQYEQEISHFV
                     SSRIVDVKPGNTKESRSIVAGLVIAMRVMKNKRGDKMAFLTLDDKSGRLEISVFADTY
                     EEYREHLIKDAVLVVEGDVSLDEYSGGLKMITRKIFSIAQARENYARLVSLQVSHARL
                     GLDFPERLKAILAPFRDGACKVRIDYQREGAKGRLLLGDDWRIAPRPELIRQLEQLCG
                     ERSLTVFYR"
     gene            complement(4927..5538)
                     /gene="rnhB"
                     /locus_tag="VFX11_11360"
     CDS             complement(4927..5538)
                     /gene="rnhB"
                     /locus_tag="VFX11_11360"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX4939266.1"
                     /translation="MAKNSTQSSFSFYTGHLVAGVDEVGRGPLAGAVVAAAVILDPEK
                     PIAGLADSKKLSEKKRDYLFDIIQRDALAWAIGRAEVEEIDTLNILHASLLAMTRAVE
                     QLQPQAEFALIDGNRCPRLQIPSEAIVKGDLTVPCISAASIVAKVTRDREMDRLDREF
                     PGYGLAQHKGYPTQSHVDALQKLGPSVIHRRTFGPVAALLEKR"
     gene            complement(5542..>6648)
                     /gene="lpxB"
                     /locus_tag="VFX11_11365"
     CDS             complement(5542..>6648)
                     /gene="lpxB"
                     /locus_tag="VFX11_11365"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX4939267.1"
                     /translation="ISAIKSRHPDAKFVGIGGPAMMAAGCESFFPMDRLSVMGVVEVL
                     GRLRELLHIRKTLREHFLANPPDVFIGIDSPDFTLPLEHSLKQAGIRTVHYVSPSVWA
                     WRQKRVFKIKEGVDLMLTLLPFEAAFYEKYKVPVKFVGHPFATAIDPSIDFARAKRYW
                     GFQSTDKVVAVLPGSRGGELKFMGPIFLETMRQLYIMEPSCKFIVACANAQRRAQLEQ
                     QLAQFDTRLPIQIVDGHAREVMAGSDIVLATSGTVTLEAMLLKRPMVVAFRWGAITHA
                     IISRLVKTRFIALPNLLAGKALVPEFLQDEARPEKLAKALFDLLRNPDNRERLQREFA
                     ELHTILNRNASEEAAKAILTLVGRPPASTSKQAI"
BASE COUNT         1314 a         1986 c         1932 g         1416 t
ORIGIN      
        1 cgcggctatc agcagcgcga atatgccaaa caggatttgc agaatgcggc cgtgcagcag
       61 gtctgccaag gtggcaccca gcagcgcccc ggccaccagg ccgactgtaa accaggtcag
      121 caccgaccag agcaccgagc ccttgcgatg atgctggaat accgagccgg tggaggtgat
      181 gatgatggta gccagagaag tgcccactgc catgtgggtc agtacctcgg acgagaatcc
      241 gagagcgcta aaggaataga tcaggacggg gacgacgatg atgcctccac ccactcccag
      301 cagccccgcc gagaggcccg caaatacacc caggagtgga tagaggagaa attccagcaa
      361 gttgctagcc gctgaatccg tagctcatga ggcgatcgta acgcctgttc agcaacgcgt
      421 cttccggcat tttctgcagg cggtcgagat tttccaccag ggcagccttc agccgctggg
      481 cggtggtgtc gtggtcgcga tgtgatccgc ccaggggttc gggaatcacc tgatccacaa
      541 tccccagctc atgcagacgc gcagcggtca ggcccatcgc ttctgccgca tcggccgcga
      601 attccgcgct tttccacaga atggacgcgc agccttccgg agaaataacg gaataggtgc
      661 tgaattgcag catctgcaga tggtcgcaca cgccgatggc cagcgcaccg ccggaaccac
      721 cctcgccgat caccgtggcg atgagggggg tgcgcaggct ggccatgacc gccagattgc
      781 gggcaatggc ttcgctctgg ccgcgttctt cagcctggat gccggggtag gcaccggggg
      841 tgtcgataaa ggtcagcacg ggcagcttga agcgctccgc cagctgcatc aggcgcatcg
      901 ccttgcggta accctcgggc tggggcatgc cgaaattgcg gcgtaccttt tccttcacgt
      961 cacggccttt ctggtggccg atgatcacca ccggcttgcc gtccaggcgg gcggtgccgc
     1021 cgatgatggc cttgtcgtcg ccaaaggtgc ggtcgccgtg cagttcatcg aattcggtga
     1081 atatgcggtc gacgtaatcc agggtatagg gacgcttggg atggcgggcg agctgggcga
     1141 tctgccagga actgagcccg gagaaaatgg tggcagtgag cgcgtggctc ttttctttca
     1201 gcagcttgat ttcgtcagtc agattgaggg cggcattgtc tttggtgacg ccgctcagct
     1261 cattgatctt ggattccagt tctgcgatgg gctgttcgaa atccagatag ttaggattca
     1321 taaatcggtg atcctgcgtt ctttttattg accttggcca gagggcatac cttacgtgaa
     1381 gctgccgtgg gtgtcgagat tgcaggaggc cgggctgccg ccctttttgt ggcaaattgt
     1441 taggcggccg ccggctgatt cagcgataaa acaccgtgag ggaacgctca ccgcacaact
     1501 gttccagctg gcggatcagt tcgggacggg gagcaatgcg ccagtcgtca cccagcagca
     1561 gacgcccttt cgcaccttct ctctggtagt cgatacgcac cttgcaggcg ccgtcccgga
     1621 acggcgccag aatggctttc agccgctcgg ggaaatccag acccagacga gcatgactga
     1681 cctgcaggct caccaaccgg gcgtagtttt cccgcgcctg ggcaatgctg aaaatcttgc
     1741 gggtgatcat tttcaggcca ccggaatatt cgtccaggct gacatcacct tccacgacca
     1801 gtaccgcgtc cttgatgaga tgctcacggt attcctcgta ggtatcggca aagacggata
     1861 tttccagccg gccggatttg tcgtccaggg tcaggaatgc catcttgtcg ccgcgcttgt
     1921 ttttcatcac ccgcatggcg atgaccaggc cagccacgat actgcgggat tccttggtgt
     1981 tgcctggttt gacatccacg atacgactgg acacgaaatg ggagatctcc tgctcgtact
     2041 ggtcgatggg atggccggtg aggtacaggc ccaacgtatc cttttcaccg ttaagacgtt
     2101 cgtcgtcgct ccagggtttg actttggccc attccggctc ggcggcggtt tcctcctcga
     2161 tggcgccaaa cagatcgacg atgccgatat cctggttttt cgatgcctgc tccgccattt
     2221 tggtggcttc gttcaggctg gccatcagcg tggcgcgatc gggcccaagc tcatccagcg
     2281 cgccggcgcg gatcagcgct tccagcgccc tgcgattgag ctttttcata tcgacacggc
     2341 ggcagaattc gaacagatcc ttgaagggtt tgccggaatt gcgcgcttcc agaatggcgt
     2401 cgatggggcc ttcgcccagc cccttgatgg cgcccaggcc atagatgatg cggccgcgtt
     2461 cgtcgacggt gaatttgtat tgggagtagc tcacatctgg cggcactatc accagtttca
     2521 tgcggcggca ttcgtcgatc agtgtcacca ccttgtcggt gttgtgcata tccgctgaca
     2581 gcaccgccgc catgaatccg gcaggatgat gggtcttcag ccaggcggtc tgatacgaca
     2641 ccagtgcata cgcagcagag tgggatttgt tgaaaccgta tttggcgaat tcttccatgg
     2701 tgtcgaagat ctcgcccgcc agcttttcat caacactgtt tttcgccgcg ccgtcgatga
     2761 agaatgcgcg ctgttttgcc atttcatcgg ctttcttctt acccatcgcc cgacgcagca
     2821 ggtcggctcc gccgagggta aagttcgcca gcacctgcgg aatcagcatg acctgttcct
     2881 ggtacaggat ggtgccgtag gtggtgttca ggattggctg cagatccgga tgcggataca
     2941 ccactttctg cttgccgtgt ttgcgcgcga tgaaatcgtc caccatgccg gaacccagcg
     3001 gaccgggacg gaacagtgcc accagcgcaa cgatatcttc aaaattggat ggcttcaggc
     3061 gcttgatcag atccttcatg ccacgggatt caagctggaa caccgctgtc gtttcagccc
     3121 tctgcaacag ttcgaacgtg gccctgtcgt cgagcgggat gcgctcgatc tccaccggtt
     3181 tctcgccgcg tttttccagc accgggttaa tggtcttcag cgcccagtca acgatggtga
     3241 gggtgcgcag gcccagaaag tcgaacttca ccaggccggc ggcttcgacg tcgtccttgt
     3301 cgaactgcgt caccaggttg ccaccttcgg catcgcaata cagcggcacg taatctgtca
     3361 gtgccgacgg tgcgatcacc acgccaccgg cgtgcttccc gacgttacgg gtgatgcctt
     3421 ccagcttcag cgccatgtcc agcagttcac ggatatcgtc gtcgcggtcg tacaattcct
     3481 tcagctgcgg ttcctgctcc atcgccttgg acaacgtcat gcccacttcg aagggaatca
     3541 gcttggcaat gcggtccaca aagccatagg aacggccctg cacgcggccc acatcacgca
     3601 ccaccgcctt tgccgccatc gaaccgaagg tgatgatctg gctcacggcg ttgcggccgt
     3661 atttttctgc cacatagtcg atgacgcggt cgcgaccgtc catgcagaag tcgacgtcga
     3721 agtcgggcat cgatacccgt tccggattga ggaagcgctc gaacagcaga tcgtattcca
     3781 gcggatcaag atcggtgatt ttcagcgcat acgccaccag cgatcccgca cccgaccccc
     3841 gccccggtcc taccggcaca ccgttgttct tcgcccactg gataaagtcg gcaacgataa
     3901 ggaagtaacc ggaaaagccc atcttgacga tacagtccag ctccacttgc aggcgctcgt
     3961 cgtagggttt acgaatgtca ctgaatgtcg gatcgttgac atcgaacagg aacgccagcc
     4021 gctcctccag ccccttgcgt gattccacca ccatgaagtc ggcaatggtc atgccgtcgg
     4081 gcacaggata ttcaggcagg taataggttc ccagcggaat gttcacgctg cagcgcttgg
     4141 ctatctccac ggtgttttcg atggcttcgg gaatgtcagc aaacaattcc accatttccg
     4201 cagcaggctt cagatattgc tcttcggaat agcgctgctc gcggctggga tcttcaagcg
     4261 tccagccttc ggaaatacag acccgcactt cgtgggcttc gaaatcgctg cgtttcagaa
     4321 aacgcacgtc attggtggcc actacggggc aatcggtttt cgcagcgagg tccaccgcga
     4381 gatgcagata ggtttcgtca tccttgcgct gggtgcgctg cagctccaga tagaaactgt
     4441 ccgggaacag atccatcatt tcctgtgtga gcagctcggc acggtccagt ttgccaccca
     4501 gcagcgcccg gccgatctca cccgacttgc cacccgacag acaaatcaag ccatctgatt
     4561 tctgcttcag ccagtcacgg gaaatgatca cccgattgcg ttgctgacca aacagataac
     4621 ccttggatac cagcacggtg agattgcgat aaccctgcag gtttttcgcc agcagcacaa
     4681 tctgggaggg gggctcatcc gtctgcgcgg cttccagcca gacatcggcg ccgtaaatgg
     4741 gcttgatacc cgcttccgtt gccgccttct ggaatttcac cagcgcgaac atgttgctgt
     4801 agtccgtgat ggcgatggcc ggcataccga gggccgcagc ggttttcgcc aatggcttga
     4861 cgcgcaccag gccgtccaca agggaatatt cggtgtgcat gcgcagatgg acgaattgcg
     4921 gtgtcatcaa cgtttctcca gcagcgccgc caccggaccg aacgtgcgcc ggtgaatcac
     4981 ggatggcccc agtttctgca atgcgtccac gtgggactgg gtgggataac ctttgtgctg
     5041 cgccagacca tagccgggaa attcccggtc cagccgatcc atttccctgt cccgtgtcac
     5101 cttggcgacg atcgacgcgg cgctgatgca gggcacggtc aaatcccctt ttacgatcgc
     5161 ctcggacgga atctgcagac gcgggcagcg gttgccgtcg atcagcgcga attccgcttg
     5221 cggctgcaac tgctccaccg cccgcgtcat ggccagcagg ctggcgtgca gaatattcag
     5281 ggtatcgatt tcttccacct cggcgcggcc gatggcccag gccagggcat cacgctggat
     5341 gatgtcgaac aggtaatcgc gtttcttttc cgagagtttt ttggaatcgg ccagccccgc
     5401 aatgggtttt tccggatcga gaatcaccgc ggccgctact actgcaccag ccagtggtcc
     5461 gcgtcccact tcatcaacac ctgcgaccag gtgaccggtg taaaaactga aactggattg
     5521 ggtggaattt tttgccatgg ttcagatcgc ctgttttgag gtggacgcag gtggccgtcc
     5581 caccagcgtc agaatggctt tcgccgcttc ctcgctggca ttgcgattga ggatcgtgtg
     5641 cagctcggca aattcccgct gcaaacgctc gcgattgtcg ggattgcgca gcaggtcgaa
     5701 cagggccttg gccagttttt ccggccgggc ctcgtcctgc aggaattccg gcaccagtgc
     5761 tttacccgcc agcagattcg gcagcgcaat gaaccgggtt ttcaccaaac gggaaatgat
     5821 tgcgtgggta atggcacccc agcgaaatgc caccaccatg ggccgtttca gcagcatggc
     5881 ctccagggtg acggtaccgg acgtggccag cacgatatcg ctgcctgcca tcacctcgcg
     5941 ggcgtggcca tccacaatct ggatcggcag gcgcgtgtcg aactgcgcca gctgttgttc
     6001 cagttgtgcg cggcgctggg cattggcgca cgccacgatg aacttgcagg aaggttccat
     6061 gatgtacagc tgacgcatgg tttccagaaa aatcggtccc atgaatttca gctcgccgcc
     6121 gcgactgccg ggcaacaccg ccaccacctt gtccgtggac tggaaccccc agtaacgctt
     6181 ggcgcgcgcg aaatcgatgc tgggatcgat ggcagtggca aatggatggc cgacgaattt
     6241 caccggcacc ttgtactttt cgtagaacgc cgcctcgaac ggcaacagcg tcagcatgag
     6301 gtcgacgcct tccttgatct tgaatacccg tttctgccgc caggcccaca ccgacggact
     6361 gacgtaatgc acggtgcgaa tgcccgcctg cttgagactg tgctccagcg gcaaggtaaa
     6421 atccggcgag tcgatgccga tgaaaacatc cggcggattt gccagaaaat gttcgcgcag
     6481 ggttttgcgg atatgcagca actcccgcag gcggccaagc acttccacca cgcccatcac
     6541 gctcaatcgg tccatgggga aaaaggattc gcagccggct gccatcatgg ccgggccgcc
     6601 gatgcccacg aactttgcat cggggtgacg ggatttgatg gcgctgat
//
LOCUS       DASUEG010000605         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_941043, whole genome shotgun sequence.
ACCESSION   DASUEG010000605 DASUEG010000000
VERSION     DASUEG010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941043"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..481
                     /gene="secG"
                     /locus_tag="VFX11_11370"
     CDS             50..481
                     /gene="secG"
                     /locus_tag="VFX11_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX4939268.1"
                     /translation="MIETILLVVLVCVALALIGLVLIQQGKGADAGASFGSGASQTVF
                     GSAGSGNFLTKSSWSLGIVFFACCLALGYIARQNAENAGRIDFSETPVEQVAPGAASD
                     VPALPASDAAVPATPDSDVPAPEASVPAAPAAETPQVPAPN"
     gene            562..646
                     /locus_tag="VFX11_11375"
     tRNA            562..646
                     /locus_tag="VFX11_11375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:596..598,aa:Leu,seq:gag)
     gene            801..877
                     /locus_tag="VFX11_11380"
     tRNA            801..877
                     /locus_tag="VFX11_11380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Met,seq:cat)
     gene            1066..1530
                     /gene="rimP"
                     /locus_tag="VFX11_11385"
     CDS             1066..1530
                     /gene="rimP"
                     /locus_tag="VFX11_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX4939269.1"
                     /translation="MSSSKRIEVLKELLEPAVHAVGMQLWGIEFLAQGKHSVLRLYID
                     SEHGVTIEDCEAVSHQVSGVLDVEDPIKSEYHLEVSSPGMDRPFFSFEQIVRYVGENV
                     KVRLQMAIDGRRNFTGTLTEAKDNTLTFQVEGQPVTVQFNQIDKANLVPTFD"
     gene            1571..3064
                     /gene="nusA"
                     /locus_tag="VFX11_11390"
     CDS             1571..3064
                     /gene="nusA"
                     /locus_tag="VFX11_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX4939270.1"
                     /translation="MSKEILLVADTVSNEKGVDKDVIFQAVELALATATKKRFPNDDV
                     EIRVSINRQTGAYDTFRRWLVVADEDFEFPGRHLTLDEAHDFDPKLKIGDIFEEPIES
                     IDFGRIAAQTAKQVIVQKVREAERQQVIEAFLDRIGELISGTVKKVTRDSIILDLGNN
                     AEALLSREEMIPRETVRVGERVRAVLWGVNKENRGPQLFVSRTRPQMLIELFKIEVPE
                     IGEDLIEIKGAARDPGARAKIAVKTHDQRIDPVGACVGMRGARVQAVSNELGGERIDI
                     VLWDDNPAQFVINAMSPADVVSIVVDEDKHSMDIAVEEEQLAQAIGKGGQNIRLASEL
                     SGWDLNVMTVSQAQNKQQEEAGAFIEVFMKELDIDEDVAGVLVDEGFTSIEEVAYVPK
                     EEMLNIDGFDEDIVDELRRRAKDALLTRALVSEEKLESDQAPAEDLLNMDGMDKITAY
                     ALAKKGIVTMDDLAEQAVDDITDVEGIDADRAAKLIMTARAPWFENQ"
     gene            3122..>4179
                     /locus_tag="VFX11_11395"
     CDS             3122..>4179
                     /locus_tag="VFX11_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4939271.1"
                     /translation="MAEVTVAQLADVVGIPVETLLQQMKDAGLAHEDAAQSVSDTEKE
                     QLLAHLKRSHGDSDSEPKKITLKRKSVSQLKVSGSQGRSKTVSVEVRKQRTYVKRSAV
                     EMEEAARQAEEEAARKAEEDRLAAIEAARKAEEARIAAEKKAAEDAARRAAAEEARAS
                     GKKGEKADSDEPKKQAQKKAPERPQVEDPAELARKAEEARIREAQRKRKEEEAAKRTL
                     EEARRMAVELEYRSGRDEFQEELLEEEKDAIVKRAFEDSLDSEARQAKRARKTSKKVK
                     GKEKQPERHERQFALRVNKHGFQSPTGPIVREVQLGESITVSDLAQQMSVKGAAVVKA
                     LFGLGIMATINQVLDRETA"
BASE COUNT          976 a         1077 c         1295 g          831 t
ORIGIN      
        1 caagaatttt tggcgatctg ccaggcggca ggataatcag gaaaacccta tgatcgaaac
       61 gatattgctt gttgtgctgg tgtgcgtggc cctggcgctg attggactgg tgctgattca
      121 gcaagggaaa ggtgctgacg ccggcgcatc cttcggttca ggtgcgtcgc agaccgtatt
      181 cggcagcgcc ggctccggta actttcttac caagtcttca tggtcgttgg gcatagtgtt
      241 ttttgcctgc tgtctggccc tgggttatat cgcgcgccag aatgctgaga atgccggccg
      301 tattgatttc agtgaaactc cggtcgagca ggttgcgcca ggtgcagcgt ctgacgtgcc
      361 cgcgttgccc gcgtcggatg ctgctgtgcc tgcgacaccg gattccgatg tgccggcacc
      421 ggaagcgtcg gtgcctgccg cgcccgctgc ggagactccg caggtccctg cgcccaactg
      481 atttccgaag cctgtcaccg gcgtaaattg ttgataattt gctggaaccc ggggatgagt
      541 ttgggtacat tatcgctccc cgccgaagtg gtgaaattgg tagacacgct atcttgaggg
      601 ggtagtggcg caagccgtgc cggttcaagt ccggccttcg gcaccaacat tcaaagcccg
      661 gcactgtccg ggctttattg tttgcgcggg tttcggccct ggccgttttt ttcaatggcc
      721 atcgatactt agcgaaatat tttattggca ctatgtgttg acatcaaaag gcaacttcaa
      781 tatagtgcgc gtcttgccga tgcggggtgg agcagcctgg tagctcgtcg ggctcataac
      841 ccgaaggtcg ttggttcaaa tccagccccc gctaccaatc aaaacaagag cccctttcaa
      901 ggggcttttt gttaacagca gtactggcag gcgacaggtg ttcggtagat gatataaatg
      961 cattctctgg acacgcagtg gatctgtcag cgtagtggtg ggcctcgtgc ccactttttg
     1021 tttctgttgt ttgtacagtc cagaccggtt caaaggtgag tccgagtgtc gtcatccaaa
     1081 cggattgaag tgctgaaaga attgctggag cctgccgtgc atgcggtggg catgcagctc
     1141 tggggcattg aattcctggc gcagggcaag cattcagtgc tgcggttgta cattgattcc
     1201 gagcatggcg tcaccatcga ggattgtgaa gccgtctcgc atcaggtcag tggcgtgctg
     1261 gatgtggaag atccaatcaa gagtgaatac catctcgaag tttcctcgcc tggcatggac
     1321 cggccatttt tcagtttcga acagatcgtc cgttacgtgg gagaaaacgt gaaggtgcgt
     1381 ttgcagatgg ccattgacgg tcgtcgcaat ttcaccggca cgctgaccga agccaaggac
     1441 aatacgctga cgtttcaggt ggaaggccag ccggtaacgg tgcagttcaa tcaaatagac
     1501 aaggcaaacc tggttccaac attcgactga cgcatgttcc gattgcaagg tctctgggtg
     1561 aggcagtgaa atgagcaaag aaatacttct ggtagctgat accgtctcca acgaaaaagg
     1621 cgtcgacaag gacgtcatct ttcaggccgt cgaactggcc ctggctactg ccaccaagaa
     1681 acgttttccc aatgatgacg tggaaatccg cgtcagcatc aaccgccaga ccggcgccta
     1741 cgacaccttc cgtcgctggc tggtggtggc cgatgaagat ttcgagtttc ccggccgtca
     1801 cctgacgctg gatgaagccc atgatttcga tcccaaactg aagattggcg acatttttga
     1861 agagccgatc gaatccattg attttggacg tatcgctgca cagaccgcca aacaggtcat
     1921 cgtgcagaaa gtgcgtgaag ccgagcgcca gcaggtgatc gaagccttcc tggaccgaat
     1981 tggcgaactg atcagcggca ctgttaagaa agtgacgcgt gacagcatta tcctggatct
     2041 gggcaacaac gccgaggcgt tactgtcccg cgaggagatg atcccccgcg aaaccgtgcg
     2101 cgtgggcgag cgtgttcgcg ccgtgctttg gggtgtgaac aaggaaaacc gcggtccgca
     2161 gctgttcgtc agccgcaccc gtccccagat gctgatcgag ctgttcaaga tcgaagtgcc
     2221 ggaaatcggc gaagacctga tcgaaatcaa gggcgcagcc cgtgatccgg gtgcccgcgc
     2281 gaaaatcgcg gtgaagaccc atgaccagcg tattgatccg gtgggtgcgt gcgtcggtat
     2341 gcgcggcgcg cgggttcagg cggtgtccaa cgagctgggt ggcgagcgca ttgacatcgt
     2401 gctgtgggac gacaacccgg cccagttcgt gatcaatgcc atgtcgcccg ctgacgtggt
     2461 gtccatcgtg gtggatgaag acaagcattc catggatatc gccgtggaag aggagcagct
     2521 ggcacaggcc atcggcaagg gcggccagaa catccgtctt gcgagcgagc tgtccggttg
     2581 ggatctgaat gtgatgaccg tgtcccaggc tcagaacaag cagcaggaag aagccggcgc
     2641 ttttattgaa gtgttcatga aagaactgga tattgatgaa gacgtggctg gcgtgctggt
     2701 ggatgaaggt ttcaccagta tcgaggaagt ggcctacgtt cccaaggaag aaatgctgaa
     2761 tatcgacggt ttcgacgaag atatcgttga tgaattgcgc cgccgcgcga aggatgccct
     2821 gttgacccgc gcgctggtgt cggaagaaaa gctggaaagc gatcaggcgc cggcggaaga
     2881 cctgctgaac atggacggca tggataaaat caccgcgtat gcgctcgcga aaaaaggcat
     2941 cgtcaccatg gacgatctgg cggaacaggc cgttgacgat atcacagacg ttgaaggcat
     3001 cgacgcggac cgcgccgcca aactgattat gactgcacgt gccccctggt ttgaaaacca
     3061 gtgatgttat gaaccggtaa attcaatcag gcacgtgaac caggagtgag agacgctgaa
     3121 tatggcagag gttacagtcg cacaacttgc agatgtggtg gggattcctg tggaaacctt
     3181 gttgcagcag atgaaggatg cggggctggc ccacgaagat gcagcgcaat ctgtatcgga
     3241 caccgaaaag gaacaactgt tggcgcacct gaaaaggtcc cacggtgaca gtgattccga
     3301 gcccaaaaaa attaccctga agcgcaaaag cgtcagccag ctgaaagtca gtggatcgca
     3361 gggccgtagc aagaccgtga gcgtggaagt ccgcaagcag cgtacctacg tgaagcgatc
     3421 ggcggtggaa atggaagagg ccgcccgcca ggccgaggaa gaagccgccc gcaaggcgga
     3481 agaagatcgt ctggctgcca ttgaagcggc ccgtaaagct gaagaagccc gtattgcggc
     3541 tgaaaagaaa gcggctgagg atgcagcccg tcgggctgct gctgaagaag cacgcgcttc
     3601 cggcaagaaa ggcgagaaag cagacagcga cgaaccgaaa aagcaggcgc agaagaaagc
     3661 gccagagcgc ccgcaggttg aagatccggc cgagctggcc cgcaaggctg aggaagcccg
     3721 catccgcgag gcccagcgca agcgcaagga agaggaagcc gccaagcgca cgctggaaga
     3781 ggcccggcgc atggccgtgg agctcgaata ccgcagcggg cgtgacgaat tccaggaaga
     3841 gttgctggaa gaggaaaagg atgccatcgt caagcgtgct ttcgaggaca gcctcgacag
     3901 cgaggcccgg caggccaagc gcgctcgcaa gacctccaaa aaggtcaagg gcaaggaaaa
     3961 gcagcccgag cgtcacgagc gccagtttgc gctccgcgtc aacaaacacg ggttccagag
     4021 cccgacaggt cccatcgtgc gagaagtcca actgggtgaa agtattaccg tttccgacct
     4081 ggctcagcag atgtccgtca agggtgccgc tgtagtcaag gccttgtttg gcctgggtat
     4141 catggccacc atcaaccagg ttctcgatcg tgaaacggc
//
LOCUS       DASUEG010000606         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_501435, whole genome shotgun sequence.
ACCESSION   DASUEG010000606 DASUEG010000000
VERSION     DASUEG010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501435"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFX11_11400"
     CDS             complement(<1..362)
                     /locus_tag="VFX11_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX4939272.1"
                     /translation="MISSAALRVLQLLLVLLLASCAATPPPAPAYVPEATGDLGVVIE
                     RGTGSVLIINTSDRTVIGRVEGLGDLSHASVVYSRNERFAYVFGRDGGLTKVDLLLRK
                     IDRRVLQGGNSIGGAISQ"
     gene            complement(359..679)
                     /locus_tag="VFX11_11405"
     CDS             complement(359..679)
                     /locus_tag="VFX11_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4939273.1"
                     /translation="MTAVVAGMSGLVLGIVAEGVTATPDAARQQQLLHLLKQDCGSCH
                     GMTLQGGLGPDLLASRLAALPREFLVTTIAKGRPGTPMPPWETLLSADDIGWLVDALQ
                     RGVE"
     gene            926..1966
                     /locus_tag="VFX11_11410"
     CDS             926..1966
                     /locus_tag="VFX11_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HEX4939274.1"
                     /translation="MRTNPLGRTGLQVTDICLGTMTWGQQNTQDEAFAQLDAALDYGV
                     NFMDTAEMYSVPTQGPTYGATETIIGNWFKARGTRDKFILATKIAGPGPWLPHIRNAE
                     TRFDRKNLEAALHGSLKRLQTDHIDLYQLHWPDRNTNFFGRLGYKHNPNELITPIEDT
                     LAVLDDLVKAGKIRHIGVSNETPWGVMKFVQLAESRGWPRIASIQNPYSLLNRSFEVG
                     LAEVAIREQAGLLAYSPLGFGMLSGKFLNGQWPETARLTLFKQFSRYTNPQAFAATER
                     YIAIAKQYNLDPCQMALAFVRMQPFTTSTIIGATSMAQLETNLRSSEVELSKEIVKAL
                     DEVHVLHPSPAP"
     gene            complement(1982..3088)
                     /gene="zapE"
                     /locus_tag="VFX11_11415"
     CDS             complement(1982..3088)
                     /gene="zapE"
                     /locus_tag="VFX11_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX4939275.1"
                     /translation="MTPLEKYQQDLKRPDFSYDAAQEQAVQHLDRLYHDLVDRYENRP
                     RSFLARLGVSKAQQTPCQGLYFWGGVGRGKTYLVDTFYECLPFRRKLRIHFHRFMQRV
                     HHELKALQGEKNPLDIVAKKLADETRVLCFDEFFVTDITDAMILGGLMEKLFSHGVTL
                     VATSNIVPDQLYKDGLQRARFLPAIALLNKYTLVVNVDSGIDYRLRALEQAEIYHSPL
                     DAAAESSLEHSFVSLAPELGEHPGREGGEIDINGRPIPVRRVADDVLWCDFRALCDGP
                     RSQNDYIELARIYHAVLVSGVPKMGAAQDDIARRFVNMVDEFYDRNVKLIMSGETGID
                     DLYGGGRLEFEFQRTRSRLLEMQSHEYLARPHRP"
     gene            3317..3751
                     /locus_tag="VFX11_11420"
     CDS             3317..3751
                     /locus_tag="VFX11_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4939276.1"
                     /translation="MSEIFTKSMARNIYLGGSVFFVLVFLGLTFDTTQRLPKTDNRAN
                     LTEQVALGKMVWERNNCIGCHTLLGEGAYFAPELGNVYQRFGQSKEAIKGFIKSRPVE
                     GIPGRRSMPQFNLTEEELDAIAEFLKYSSEINTNNWPPNING"
     gene            3764..5173
                     /locus_tag="VFX11_11425"
     CDS             3764..5173
                     /locus_tag="VFX11_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX4939277.1"
                     /translation="MSEVQLRYKTQAVAKPYFIAAIGLFVGQVIFGLIMGLQYVIGDF
                     LFPEIPFNVARMVHTNLLIVWLLFGFMGAAYYLVPEEAERDIPSPKLALALFWIFLVA
                     GALTIVGYLAVPYATLAKITGNDLLPTMGREFLEQPTITKIGIVIVALGFLYNIGLTI
                     LGGRKTVINVVLLTGLVGLAVMFLFSFYNPDNLVLDKYFWWWVVHLWVEGVWELILGS
                     VLAYVLIKTTGVDREVIDKWLYVIIAAALITGIIGTGHHYFWIGTPTYWTWLGSVFSA
                     LEPIPFFMMTIFAFNMVNKRRKQHPNKAAVLWAMGTGVMAFLGAGVWGFMHTLAPVNY
                     YTHGTQVTAAHGHMAFYGAYAMIVMTIISYAMPKLRGMTAMSNRAQVMEMWSFWLMTI
                     AMVFITLFLTAAGILQVWLQRYQPNALPFMAAQEQVAIFYWCREAAGVIFTIGLLCYI
                     ASFFVKGDSEGTTAEPVAS"
BASE COUNT         1053 a         1608 c         1554 g         1040 t
ORIGIN      
        1 tcctggctga tggcgccgcc gatgctgttg ccgccctgca ggacgcgacg gtcgattttc
       61 cgcagcagca gatcgacttt ggtgagtccg ccatcgcggc cgaacacata ggcaaaccgc
      121 tcgttgcggg aatacaccac cgaggcgtgg gacagatcgc ccagtccttc cacccgacca
      181 ataacggtcc ggtcactggt gttgatgatg aggacggaac cggtgccccg ttcgatgacg
      241 acaccgagat caccggtggc ttccggaaca taggccggcg cgggcggtgg cgtggcagcg
      301 caagaggcca gcagcagtac caacaggagt tgcagcacgc gcaaagcagc agacgatatc
      361 attcgacacc ccgctgcagc gcgtccacca accagccgat gtcatcggcg ctgagcagcg
      421 tttcccaggg cggcatcggc gtgcccggtc gccccttggc gatggtggtc accagaaatt
      481 cacggggcag cgctgccagg cgactggcca gcagatccgg gcccaagcct ccctgcaggg
      541 tcatgccgtg gcaggaaccg caatcctgct tcaataaatg cagcaactgc tgctgccggg
      601 ctgcgtccgg cgtggcggtc accccctcgg ccacaatacc cagcaccaac cccgacatac
      661 ctgccactac cgctgtcatt cgcctgaacc gcaccgtgct tcctccatgg acactattgc
      721 atggacactc gcagcccatt gtaggaacca agcccgactc cagtttgcgc tgtgtcagct
      781 tttacccagc ctctaccctt cagaacgggc ttttacccac aaagaggtat gctccccatt
      841 gggttgccat cccttttgtg acagccactt agccgttacc atcggcaggt cgccgtcact
      901 tcgatcacag gggaaaccat ccgccatgcg cacgaatcca ctgggccgca ccggcctgca
      961 ggtcaccgac atctgcctgg gcactatgac ctggggccag cagaacaccc aggacgaagc
     1021 cttcgcccaa ctggacgccg cactggatta cggcgtgaat ttcatggaca cggccgagat
     1081 gtattcggtg cccacccagg ggccgactta cggcgccacc gaaaccatca tcggcaactg
     1141 gttcaaggcg cggggcaccc gggacaaatt catcctggcc accaagatcg ccggacccgg
     1201 cccctggctg ccccatatcc gcaacgccga aacgcgattc gaccgcaaga atttagaggc
     1261 agcattgcac ggcagcctga agcgcctgca gaccgaccac atcgacctgt accagctgca
     1321 ctggcccgac cgcaacacca acttcttcgg ccggctgggt tacaagcaca accccaatga
     1381 actgatcacc cccatcgaag acacgctggc ggtactggac gatctggtga aagcgggaaa
     1441 gatccgccat atcggggtgt cgaacgaaac gccctggggc gtgatgaagt ttgtgcagct
     1501 ggcggaaagc cggggctggc cgcgcatcgc gtcgatccag aatccgtaca gtctgctgaa
     1561 ccggtcattt gaagtggggc tggcggaagt ggcgatccgg gaacaggcgg ggttgctggc
     1621 gtattccccg ctgggattcg gcatgctgtc gggcaagttc ctgaatggtc agtggcccga
     1681 aacggcgcgc ctgaccctgt tcaagcagtt ctcgcgctac accaatcccc aggcctttgc
     1741 cgccaccgag cgctatatcg ccatcgcaaa gcagtacaac ctcgaccctt gccagatggc
     1801 gctggccttc gtgcgcatgc agccgttcac cacgtccacc atcatcggcg caacgtcgat
     1861 ggcgcagctg gaaaccaacc tgcgcagcag tgaagtggaa ttgagcaagg agatcgtgaa
     1921 agcgctggat gaggtgcatg tgctgcaccc ctcccctgcg ccctgatgca tttccaatgg
     1981 attacggacg gtggggacgg gccaggtact cgtggctctg catctccagc aggcggctgc
     2041 gggtgcgctg gaattcgaac tccagccgcc cgccaccata caggtcgtca atgcccgttt
     2101 ccccggacat gatcagcttg acgttgcggt cgtagaactc atccaccatg ttcacgaagc
     2161 ggcgggcgat atcatcctgc gcagcgccca ttttcggcac gccgctcacc agcaccgcgt
     2221 ggtagatccg cgccagttcg atgtagtcgt tctggctgcg gggaccgtcg cacaacgcgc
     2281 ggaaatcaca ccagagcacg tcatccgcca cccgccgcac cgggatcggg cggccgttga
     2341 tatcgatttc acccccttcc cggcccggat gctcccccag ttccggcgcc agggacacga
     2401 aactatgctc cagcgagctt tccgccgctg catccagcgg gctgtggtag atctcggcct
     2461 gctccagcgc ccgcaggcgg tagtcgatgc cgctgtccac attcaccacc agggtgtact
     2521 tgttcagcag ggcaatggcg ggcaggaagc gggcccgttg cagaccatcc ttatagagct
     2581 gatccggcac gatattggag gtggccacca gggtgacgcc gtggctgaac agcttctcca
     2641 tcaggccgcc caggatcatg gcgtcggtga tgtcggtgac gaagaactcg tcaaagcaga
     2701 gtacgcgggt ctcgtccgcc agcttctttg ccacgatatc gaggggattt ttctcgccct
     2761 gcagggcttt gagttcgtga tggacccgct gcatgaagcg gtggaagtga atccgcagct
     2821 tgcgccggaa cggcaggcat tcgtagaagg tgtccaccag ataggtcttg ccgcgcccta
     2881 ccccgcccca gaaatagagg ccctggcagg gcgtctgctg cgccttggaa acgcccaggc
     2941 gggcaaggaa ggagcgggga cggttctcat agcggtccac cagatcgtgg tagaggcggt
     3001 cgaggtgctg cacggcctgt tcctgggccg catcgtagct gaagtcgggg cgcttcaggt
     3061 cctgctggta tttttcaagt ggagtcatgg catcaacact cgggttcacg gttccacggc
     3121 gggcgtcact ctacctgagg tgccctgggc tgtccacgca gtaacctgtt accaaaagcg
     3181 tacctcgcat cgcggatttg actaccccct cagaggtact gaacttcatt aggcttttct
     3241 gatcccgatc aaatttgtcg ggtatgtccg cccggatact ggttccacct ccagactgac
     3301 agacaagggt ggtgcaatga gcgaaatttt taccaaatcc atggcgagga acatctatct
     3361 gggaggcagc gtctttttcg tgctggtctt tctcggactg acgttcgaca ccacgcaacg
     3421 gctgcccaaa acggacaacc gggccaacct caccgaacag gtggcgctgg gcaaaatggt
     3481 gtgggaacgc aacaactgca tcggctgcca cacgctgctg ggtgaaggcg cctatttcgc
     3541 gccggaactg ggtaatgtgt accagcgctt tggccagtcg aaggaagcca tcaagggctt
     3601 tatcaagagc cgcccggtag aaggcattcc cggccgccgc agcatgccgc aattcaacct
     3661 gacggaagaa gagctggatg cgatcgcgga gttcctcaag tacagcagcg aaatcaatac
     3721 caacaactgg ccgccgaaca tcaacggcta aggggaggca accatgagtg aagtgcaact
     3781 tcgttataag acgcaagcgg ttgccaagcc gtatttcatt gcggcaatcg gcctgttcgt
     3841 gggccaggtg attttcggtc tgatcatggg cctgcagtat gtgatcggcg atttcctgtt
     3901 tccggaaatt cccttcaacg tggcgcgcat ggtgcacacc aacctgctga tcgtatggct
     3961 gctgtttggt ttcatgggcg cggcctatta tctggtgccg gaagaagccg agcgcgatat
     4021 tcccagcccg aaactggcgc tggcgctgtt ctggattttt ctggtggccg gcgcgctgac
     4081 gattgtcggc tatctggcag tgccctatgc aacgctggcg aaaatcaccg gcaatgatct
     4141 gctgcccacc atgggtcgtg aatttctgga gcaaccgacg attaccaaga tcggcattgt
     4201 gatcgtggcg ctgggttttc tgtacaacat cggcctcacc atcctgggcg ggcgcaagac
     4261 ggtgatcaac gtggtgctgt tgactggcct cgtgggactt gccgtgatgt tcctgttctc
     4321 gttctacaac cccgacaacc tggtgctgga taaatacttc tggtggtggg tggtgcatct
     4381 gtgggtggaa ggcgtgtggg aactgatcct cggatcggtg ctggcctatg tgctgatcaa
     4441 aaccaccggt gtggaccgcg aggtgatcga caagtggctg tacgtgatca tcgccgccgc
     4501 gctgatcacc ggcatcatcg gcacgggcca ccactatttc tggatcggca caccgacata
     4561 ctggacctgg ctgggcagcg tgttctcggc actggaaccg attccattct tcatgatgac
     4621 gattttcgcc ttcaacatgg tgaacaagcg ccgcaagcag catcccaaca aggccgctgt
     4681 gctgtgggcg atgggtaccg gtgtgatggc gtttctcggc gcgggcgtgt ggggtttcat
     4741 gcacaccctg gcaccggtga attactacac ccacggcacc caggtgactg cggctcacgg
     4801 ccatatggcg ttttacggcg cctatgccat gatcgtgatg accatcattt cctatgccat
     4861 gccgaaactg cgcggcatga cggcgatgag caaccgcgct caggtgatgg aaatgtggag
     4921 cttctggctg atgaccattg cgatggtgtt catcacgctg ttcctcactg ctgcgggcat
     4981 cctgcaggta tggctgcaac gttaccagcc caatgcactg cccttcatgg cggcgcagga
     5041 acaggtggcg atcttctact ggtgccgtga agcagcgggc gtgattttca ccatcggcct
     5101 gctctgctac atcgccagct tctttgtgaa gggagacagt gaaggtacga cagccgaacc
     5161 tgtagccagc tgacaccatg acagcgccgc ccggctgcgg gcggcgcctt tttcccgggc
     5221 gatcccttac caggtgcccc ttaccggatg acaag
//
LOCUS       DASUEG010000607         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_360357, whole genome shotgun sequence.
ACCESSION   DASUEG010000607 DASUEG010000000
VERSION     DASUEG010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360357"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFX11_11430"
     CDS             complement(<1..194)
                     /locus_tag="VFX11_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939278.1"
                     /translation="MSEFSDRVKGQLEQFNNSIDQIINASRGLYFKIKEESNKQFDEL
                     VKTGEAQKTAEESLLTQLKK"
     gene            complement(384..1715)
                     /locus_tag="VFX11_11435"
     CDS             complement(384..1715)
                     /locus_tag="VFX11_11435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4939279.1"
                     /translation="MLQGYVHPDFGKVADMLSHQIPRNRPGGAALTVYHKGQCVVDIW
                     GGTRNRDGDAWRSDTLALSFSTTKGVASTLLHILVDQGLLSYDDPVSRHWPEFGQKSK
                     QPITVRQLLCHEAGLYHIRDMIDDAEQMLDWNFMVGAIERAKPVHTPGATNGYHALTF
                     GWLIGELVQRVTGKTFAQVLQEKLAGPLELDGLYVGLPDSQVRRRAELVNSIGNGDTR
                     FQAERHLQKLLLRAITQGLKVARIDLDKTISALVPKGALYMDFNHPDFAGACIPSANG
                     VFTARSLAKLYAVLANGGELNGVRILSRESVNRIMQIQNRTLGDVIPVPMHWRLGFHR
                     VLALGVATEHSFGHFGFGGSGAWADPVRNLSAAMTLNSGIGTPFGDTRMVQITGTIVR
                     CAEKRYLVSDVEEVRGIGPFFPRERRTTYDTAKKLVTGVLPRRRSNDVSVA"
     gene            1928..2629
                     /locus_tag="VFX11_11440"
     CDS             1928..2629
                     /locus_tag="VFX11_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939280.1"
                     /translation="MSQQWYLGFGASESLYQLNKDYRASLSSSSDKEQTEKLYGLLAQ
                     FADECLDQYFLSPIESVQLNGVGKKVVLGGVSAIKKTIHLTLRQVTKKLSAQDRAQLA
                     DYINDMMMQLRQSRRFPTFIAVPVSDALRLRLGNAVQQGKAQSPASVRDDYAEALCEL
                     IDVAVEAYMHQPIRMLKMGMVMNKIATVAADTIQAAAHTVVRKVMHSMNDKEMMAMFE
                     FSESILYQPAQQKAA"
     gene            complement(2727..3704)
                     /locus_tag="VFX11_11445"
     CDS             complement(2727..3704)
                     /locus_tag="VFX11_11445"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX4939281.1"
                     /translation="MLKTAVTEMLGIQHPIVQGGMMHVGRAQLAAAVSNAGALGIITA
                     LTQPTPEDLAKEIRLCRELTDKPFGVNITLLPTIKPVPYEEYARVIIENGVKIVETAG
                     RSPEQFMPAFKAAGVKVIHKCTSVRHALKAEKIGCDMVSIDGFECAGHPGEDDIPGLI
                     LIPAAANVLKIPMLASGGFGDGRGLAAALALGADGINMGTRFVATQEAPVHENLKKRM
                     VEASELDTALIFRSLRNTARVFKNSVADKVVEIEAQPGATNFEDLAPLVAGIKGREVM
                     DKGDLEYGIWTAGMVMGLIRDIPDCKTLVTRIVSEAQEIITARLGGMVQ"
     gene            complement(3784..4239)
                     /locus_tag="VFX11_11450"
     CDS             complement(3784..4239)
                     /locus_tag="VFX11_11450"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEX4939282.1"
                     /translation="MYSEDIDARRGIAQLALKGFFNLAQRWQLSRPEAMTLLGLTATS
                     TYANWKNGKTGTLPRDTLERISYLLHIDDALRQRCQNDLHLRNWLRETHALLQQRSPL
                     GRMLEGNVIDLHWVQQQMPLLTSSLEAPALAFESKNVADQSPRATINSL"
     gene            complement(4342..>4937)
                     /locus_tag="VFX11_11455"
     CDS             complement(4342..>4937)
                     /locus_tag="VFX11_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX4939283.1"
                     /translation="LIAEISDKAPLDGYTDPEKTKKVILTDVFEKGDRWFNTGDMLRD
                     IGFGHAQFVDRLGDTYRWKGENVSTTEVENLVSDHPQISEAVAYGVEIPNTNGRAGMA
                     SITPTVPVDQLDFKDIYRFLKAQMPAYAVPLFLRVKKSMETTGTFKYQKSHLKEEGFD
                     PSKTNGEPVYALLPGSEEYVLVTDQVLADINAGKYRY"
BASE COUNT          956 a         1295 c         1565 g         1121 t
ORIGIN      
        1 tctttcttga gttgagtaag cagggattct tcggctgttt tctgggcttc gcccgtctta
       61 accaattcgt cgaactgctt gttgctttct tccttgatct tgaaataaag accgcgagaa
      121 gcattgatga tctggtcaat ggagttgttg aactgttcca gttgtccttt aacgcgatct
      181 gaaaattcgc tcatgtttaa atccctcgca cacgatttgt ttcgttgacc tcattcgaat
      241 tcccataaag ctgactcacc ctggcgacag actgcgtgcc aacaccgggc ggacaactca
      301 gcgttacagg cgtgcgatgg taaccagtgg aattagaatg ttaaccctaa aaagtgtaac
      361 gcgacgttca ttgaaatcgg ccttcacgcg acggagacat cgttgctgcg gcgcctgggc
      421 agcacgccgg ttaccagttt tttagcggta tcgtaggtgg tgcgtcgttc gcgcgggaag
      481 aaggggccga tgccacgtac ttcttccacg tcggaaacga ggtagcgttt ttccgcgcag
      541 cgcacgatgg tgccggtgat ctgtaccatg cgcgtatcgc cgaacggcgt gccaatgccg
      601 ctgttgagag tcatggcggc cgacaggttg cgcaccgggt cggcccaggc gccggagccg
      661 ccaaaaccga aatgaccaaa gctgtgttca gtggcgacgc ccagcgccaa cacgcggtga
      721 aagccaaggc gccagtgcat ggggactgga attacgtcgc caagagtccg gttctggatc
      781 tgcatgatgc ggttgacgct ttcccgcgac aggatgcgca cgccattcag ttcgccgcca
      841 ttggccagca cggcatacag tttggccagg gaacgggcag taaatacgcc gttggcggat
      901 ggaatgcagg cgccggcgaa atccggatgg ttgaaatcca tatagagcgc gcccttgggc
      961 accagtgcgg aaatggtttt gtcgagatcg atgcgggcaa ccttcaggcc ctgggtaatg
     1021 gctcgcagca gcagtttctg caggtggcgt tcggcctgga agcgggtgtc gccgttgccg
     1081 atgctgttca ccagttcggc gcggcggcgc acttgactgt ccggcagccc cacataaagg
     1141 ccgtccagtt ccagcggccc tgccagtttt tcctgcagca cctgggcgaa ggttttgccg
     1201 gtgacgcgtt gcaccagttc gccgatcagc cagccgaagg tgagggcatg gtagccattg
     1261 gtggcgccgg gcgtatgaac cggtttggca cgttcgatgg cgccgaccat gaaattccag
     1321 tccagcatct gttcggcatc atcgatcatg tcacggatgt ggtagaggcc ggcttcgtga
     1381 cacagcagtt gccgtacggt gatgggctgc ttggatttct ggccgaattc cggccagtgt
     1441 cggctgaccg gatcgtcata gctgagcagg ccctgatcca cgagaatgtg cagcagggtg
     1501 gatgcgacac ctttggttgt ggagaacgaa agggcaaggg tatcggaacg ccaggcatcg
     1561 ccgtcgcggt tgcgggtgcc gccccagatg tccactacgc actggccctt gtggtagacg
     1621 gttagcgccg cgcctccagg gcgattgcgg ggtatctgat gtgagagcat gtcggccact
     1681 ttgccaaagt ccggatgcac gtaaccctgc agcatgatca ctccctgttt ctgtttgtta
     1741 ttctgttttc aaatgtatag cagttttttg gcgttctgac ctgaaactgt caaaaatggg
     1801 gcgaactggt tttggtttgc acgagattga tattgccaac cagttacctg ggcgctgagg
     1861 tcattgggca agtcgtcact atctgcttaa attgcgcatc tttttttcac cggaatggag
     1921 ttgcgacatg agtcagcaat ggtatttggg atttggtgca agcgaatcct tgtatcagct
     1981 caataaggat tatcgtgcgt cattgtcttc atccagcgac aaggagcaga cggagaagtt
     2041 gtacggcctg ctggcacagt ttgcagacga gtgcctggac cagtattttc tgtcgccgat
     2101 tgaatcggtg cagttgaatg gcgtgggcaa gaaagtggtg ctgggcggtg tgtctgcgat
     2161 caagaagacc attcatctga cgctcaggca ggtgaccaaa aagctgtcag cccaggaccg
     2221 ggcgcagctg gcggattaca tcaatgacat gatgatgcag ctgcgccaga gccgccgctt
     2281 tcccacgttc attgccgtgc cggtgagcga tgcgctgcgg ctgcggctgg ggaacgctgt
     2341 gcagcagggc aaggcgcagt cgcccgccag cgtcagggac gattatgctg aagcgctgtg
     2401 cgagctgatc gacgttgccg ttgaggcgta tatgcatcag ccgatccgga tgctgaagat
     2461 gggcatggtg atgaacaaga ttgccacggt ggcggccgac acgatccagg cagcagccca
     2521 tacggtggtg cgcaaggtga tgcattccat gaatgacaag gaaatgatgg cgatgttcga
     2581 attttccgag tcgatcctgt atcagccggc acagcagaag gcggcgtgac ggttgcgtga
     2641 ggaggccggg aatgtcgccg gcggaccatc aaaaaggggc cttggttgcg ggcccctttt
     2701 tttgccggaa agccggagaa tagatcttat tgcaccattc cgcccagacg cgcggtgatg
     2761 atttcctgcg cttccgacac aatgcgggtg accagggttt tgcagtccgg gatgtcgcgg
     2821 atcagtccca tcaccatgcc ggcagtccag atgccatatt ccaggtcgcc cttgtccatc
     2881 acttcgcgac ctttgatgcc ggctaccagc ggtgccaggt cttcgaaatt ggtcgcgccg
     2941 ggctgggctt cgatttccac cactttatcg gccacgctgt tcttgaacac gcgggcggtg
     3001 ttgcgcaggg agcggaagat cagggcggtg tcgagttcgg acgcttccac catgcgcttc
     3061 ttgagatttt cgtgtaccgg cgcttcctgc gtggccacga atcgggtgcc catgttgatg
     3121 ccgtcggcgc ccagtgccag cgctgccgcc agaccgcgtc cgtcgccaaa accaccggag
     3181 gccagcatcg gaattttcag cacgttggct gcagcgggga tcaggatcag gccggggatg
     3241 tcgtcttcgc ccgggtgacc ggcgcactcg aaaccgtcga tggacaccat gtcacagcca
     3301 attttttcgg ccttcagtgc gtggcgcacc gaggtgcact tgtgaatgac tttcacgccg
     3361 gcggccttga acgcgggcat gaactgctcc gggctgcggc cagcggtttc cacgatcttc
     3421 acgccgtttt caatgatgac gcgcgcatat tcctcgtagg gaaccggctt gatggtgggc
     3481 agcagggtga tgttgacgcc gaaaggcttg tcggtgagtt cgcggcagag gcggatttcc
     3541 ttggccagat cttccggagt gggctgggtc agcgcggtga tgatgcccag tgcgccggca
     3601 ttggatacgg cagcggccag ctgggcgcgg ccaacgtgca tcatgccacc ctggacgatg
     3661 ggatgttgga tgccaagcat ttcggttaca gcagtcttta acatggggtt cactccgtgg
     3721 tcaggtggcg ctactacagc aaaatccact gacccgggca acgccggaac ggccccaagc
     3781 aactcagagt gaattaattg tcgcgcgcgg tgactggtcg gccacgtttt tggattcgaa
     3841 ggcgagcgcg ggggcttcca ggctggacgt cagcagtggc atttgctgct gcacccagtg
     3901 caggtcgatg acgttgcctt ccagcatgcg gccgaggggc gagcgttgct gcagcagggc
     3961 atgggtctcg cgtagccaat tgcgcaggtg caggtcgttc tggcagcgtt gccgcagggc
     4021 gtcgtcgatg tgaaggagat aggaaatgcg ttcgagggtg tcgcggggga gagtgccggt
     4081 cttgccgttt ttccagttgg cgtaagtgga agtggcggtg agtcccagca gtgtcatggc
     4141 ctccggtcgg gagagttgcc accgctgagc caggttgaag aatcccttca gggccagttg
     4201 ggcaatgccg cggcgggcat cgatgtcctc gctgtacatg gcttcacctt cgcaatgggt
     4261 tttataggtt atagcaaagg tcaaaaaaaa ggccaacacg gagggtgttg gcctttgggg
     4321 tgaaactctg tcggaactgg atcagtaacg gtattttcca gcgttgatgt ctgccagaac
     4381 ctgatcggtg acgagcacgt attcttcgct gccgggcagc agcgcgtata caggttcgcc
     4441 attggttttg gacggatcaa agccttcttc cttcaaatgg gatttctggt atttgaaggt
     4501 accggtggtt tccatcgact tcttcacgcg caggaacagc ggaacagcgt aagcaggcat
     4561 ctgcgctttc aggaagcggt agatgtcctt gaaatccagc tggtccaccg gaacggtcgg
     4621 ggtaatcgag gccatgccgg cgcggccgtt ggtgttggga atttccacgc cgtaagctac
     4681 cgcttcggaa atctgcgggt gatccgacac caggttttcc acttcggtgg tggagacgtt
     4741 ctcgcctttc cagcggtagg tgtcacccag acggtcgacg aactgggcgt ggccgaagcc
     4801 gatgtcgcgc agcatgtcac cggtgttgaa ccagcggtcg cctttctcga acacgtcggt
     4861 gaggatgacc ttcttggttt tttccgggtc ggtgtagccg tccagcggtg ccttgtcgct
     4921 gatttcagca atcagca
//
LOCUS       DASUEG010000608         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_707388, whole genome shotgun sequence.
ACCESSION   DASUEG010000608 DASUEG010000000
VERSION     DASUEG010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707388"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VFX11_11460"
     CDS             complement(<1..134)
                     /locus_tag="VFX11_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HEX4939284.1"
                     /translation="MSLFHVQPGDLVFAAHAIHNDGSIPALEDNALLAAAGARGVVLN
                     "
     gene            complement(315..1691)
                     /locus_tag="VFX11_11465"
     CDS             complement(315..1691)
                     /locus_tag="VFX11_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX4939285.1"
                     /translation="MKHNNPLKLLAGALLASSLVAAPALGKVDQARADKLGGSELTVT
                     GAPVAGNADGSIPAYTGGLKEPPACYKGGDWLCDPFADDKPLFTITAENVDQYAGKLS
                     AGQIALFRKYPKTFRMPVYPTRRTFRMPDDVAALTRENAVKTETLGATGLKNYTPKAF
                     PFPITDNGLEMVWNHIARWRGTGVDRFAGQATPQANGAYSMVMFHDEVVIPAAMTDYV
                     EGEDPNVMFYGKQTVIAPSRLAGNVLMVHETIDQVREPRRAWIYNAGQRRVRRAPQVA
                     YDGPGTAADGLRTADNYDLYNGSPDRYTWTFKGKKELYIPYNSYKLDKKGLPYDDVIN
                     AGHINQDLTRYELHRVYEVEAVLREGTRHIYARRVFHFDEDSWMAAVIDHYDNRGNLW
                     RVAEAHQMLFYQIPVQGYSVETLYDLNSGRYMVLGLENNEAQGIKWDAKFSKTDFQPA
                     AVRRAGVR"
     gene            complement(1717..>3237)
                     /locus_tag="VFX11_11470"
     CDS             complement(1717..>3237)
                     /locus_tag="VFX11_11470"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HEX4939286.1"
                     /translation="DILDAFVWGDWDMNGHPLNVRFGKQVINWGEGLLFANGINNINP
                     VDVSALLAPGSEVKDALIPLNSLYGSVGVTNALSLEAFMLFDWRETELPNCGTFFSQS
                     DLVGPGCWAGFVPSGMESSFTGNPLTPLDDAINFTLPRGADQTPDSDGQYGVAARYFV
                     ESIETEFGVYYTNLHSNLPVISGHTPDVTKLGFPTLQAARTALVGGTGNPLATIALLP
                     LGDYFVEYPEDIQMFGASFSTSQDLGLPGGATAISGEISMRKDQPFALEDGDALSGAV
                     GLPSLGCWDAPTAYDCYTDYAPGEYKAGYVQKDYFQAEMVFIHFFDQVLGASRWTAVV
                     DIAGSYLDLPSKSQALLNSNYNATLNHPWAPNVPTIAGLVPLLYPQFIDQVWQAQQAL
                     PVYARSYTVAPEGDDYFPTSGAWGYKLRFSGDYEDVFAGINLRPTISFSHDVDGTTPT
                     PIANFLEDRKAIGLSLEAVYLNDYSVNIGYTDFYGAEPYNSLADRDYYSLSTTISF"
BASE COUNT          597 a          933 c         1047 g          660 t
ORIGIN      
        1 atgttgagca cgacaccccg agcgcctgct gccgccagca gcgcgttgtc ttccagtgcg
       61 ggaatggagc cgtcgttgtg aatggcatgg gcggcaaaaa ccaggtcgcc cggctggaca
      121 tggaacaggg acatcggcgt actccgaaca gatcacaggg tcaggccgca gtccaattgt
      181 cgtgtttgcg acaataacgc ggggttggac gcggctttcc tgtgcttcag caaattctgt
      241 tccggtcgcg gattattgca ggcacaaaaa aggggtgccg cagcacccct ggggatttgc
      301 ttgcgctcag gcaactagcg cacgccggcc cggcgcactg ctgcaggctg gaaatccgtt
      361 ttcgagaatt tcgcatccca cttgatgcct tgcgcttcgt tgttttccag acccagcacc
      421 atgtagcgac cggagttcag gtcgtacagg gtttccaccg agtagccctg caccggaatc
      481 tggtagaaca gcatctggtg cgcttccgcc acccgccaca ggttgccgcg gttgtcgtag
      541 tgatcgatca ccgccgccat ccagctgtcc tcgtcgaaat ggaacacgcg tcgggcatag
      601 atgtgacggg tgccttcgcg cagcacggcc tccacttcat atacccggtg cagttcgtaa
      661 cgggtcagat cctggttgat gtgaccggcg ttgatgacgt cgtcataggg caggcccttc
      721 ttgtccagct tgtaggagtt gtaggggatg tacagctcct tcttgccctt gaaggtccag
      781 gtgtagcggt ctggcgagcc gttgtagagg tcgtagttat cggcggtgcg cagaccgtcg
      841 gcagcagtgc cgggaccgtc gtaggctacc tgcggcgccc gccgcacgcg gcgctggccg
      901 gcattgtaga tccaggcccg gcgtggttcc cgcacctggt caatggtctc gtgcaccatc
      961 aacacgttac ccgccagacg cgatggcgcg atgaccgtct gcttgccata gaacatgacg
     1021 ttggggtcct cgccttccac atagtcggtc atggcggcgg gaatcaccac ttcatcatgg
     1081 aacatcacca tgctgtaggc gccgttggcc tggggtgtgg cctggccggc gaagcggtcc
     1141 acaccggtgc cgcgccagcg ggcgatgtgg ttccatacca tttccaggcc gttgtcggtg
     1201 atcggaaacg gaaacgcctt gggggtgtag ttcttcagac cggtggcgcc gagggtttcg
     1261 gttttcaccg cgttttccct ggtcagcgcg gcgacgtcat ccggcatgcg gaaggtgcgg
     1321 cgggtgggat acaccggcat gcggaaggtc ttggggtatt tcctgaatag cgcgatctgg
     1381 ccggcgctga gtttgccggc gtactgatcg acattttccg ccgtgatggt gaatagcggc
     1441 ttgtcatccg cgaacggatc gcacagccag tcaccgccct tgtagcaggc cggcggttcc
     1501 ttcaggccgc cggtgtaggc aggaatggaa ccgtcggcgt tgccggccac gggggcgccg
     1561 gtgacagtga gttcgctgcc gcccagcttg tcggcccggg cctggtccac cttgcccagc
     1621 gccggtgcgg caaccagcga cgaggccagc agcgcgccgg ccagcagttt cagcgggttg
     1681 ttgtgtttca tgttcatatc catattggcc ccggaatcag aaggaaatgg tggtgctgag
     1741 ggagtagtaa tcccggtcag ccaggctgtt gtagggttct gcgccgtaga aatcggtgta
     1801 gccgatgttg acgctgtagt cgttcaggta caccgcttcc agcgacaggc cgatggcctt
     1861 gcgatcctcc aggaagttgg cgatcggggt gggcgtggta ccgtcgacat cgtggctgaa
     1921 gctgatggtg ggccgcaggt tgataccggc gaacacgtct tcgtagtcac cgctgaagcg
     1981 cagcttgtag ccccaggcgc cggaggtggg gaagtaatcg tctccttcgg gtgcaaccgt
     2041 gtagctgcgg gcgtacaccg gcaaggcctg ttgggcttgc catacctgat cgatgaactg
     2101 cgggtagagt aacggcacca aaccggcaat ggtgggcaca ttgggtgccc agggatggtt
     2161 caaggtggcg ttgtagttgc tgttcagcaa cgcctgggat ttgctgggca gatccaggta
     2221 ggatccggca atgtccacca cggctgtcca gcgcgaggcg ccgagaacct ggtcgaagaa
     2281 gtggatgaac accatttcgg cctgaaagta atctttctgc acatagcccg ctttgtattc
     2341 gcctggcgcg taatcggtgt aacagtcata tgcggtgggg gcatcccagc agcccagcga
     2401 aggcaggccc acggcgccgg acagtgcatc accatcttcc agtgcgaaag gctggtcctt
     2461 gcgcatgctg atctcgccgg aaattgcggt ggcgccaccc ggcaggccga gatcctggct
     2521 ggtgctgaag ctggcgccga acatctgaat gtcttccggg tattccacga aataatcgcc
     2581 cagcggcagt agcgcgattg tggccagcgg gttgcccgtg ccgccgacca gtgcagtacg
     2641 agccgcttgc agagtgggga aaccgagctt ggtcacgtcc ggcgtgtgtc ccgaaatcac
     2701 cggcaggttg gaatgcagat tggtgtagta aacgccgaac tcggtttcaa tggattccac
     2761 gaaatagcgg gcggccacac cgtactggcc atcgctgtca ggcgtctggt cggcaccgcg
     2821 tggcagagtg aagttgatcg cgtcatcaag tggcgtcagc ggattgccgg taaaggacga
     2881 ctccatcccc gacggcacga acccggccca gcagcctggc cccaccagat ctgattgcga
     2941 gaagaaggtg ccgcagtttg gcagctcggt ctcacgccag tcgaacagca tgaaggcttc
     3001 cagcgacagc gcgttggtca cgcccaccga tccgtacagg gaattcagcg gaatcagcgc
     3061 atccttcact tccgatcccg gcgccagcag cgccgaaacg tccacgggat tgatgttgtt
     3121 gataccgttg gcaaacagca ggccctcgcc ccagttgatg acctgtttgc cgaagcgcac
     3181 gttgagcggg tgtccattca tgtcccagtc gccccacaca aaggcgtcga ggatgtc
//
LOCUS       DASUEG010000609         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1681867, whole genome shotgun sequence.
ACCESSION   DASUEG010000609 DASUEG010000000
VERSION     DASUEG010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681867"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VFX11_11475"
     CDS             complement(<1..1466)
                     /locus_tag="VFX11_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939287.1"
                     /translation="MLSDTYSQDDIRNGFRAEIRSPRTALGERVKMALATLFAITLPL
                     AFVTLIGLSCLVWFQFTRDLLLNGFASRPGWAVVVVLGDLFLVGFLAAMVRRLVLIRR
                     HSREYLAVSVQSQPELHAFVNHVALLLRVPFPRAIKLDAEVGLILRPGSLKDILLGHG
                     PEILIGVPLLYGLSARQLAGVIAHAYAGYTSEARRYGYPLICAIDRWLFAQSGLARSA
                     TSIKEEFRSLDEGRSRQRLAASLRPFDFLVQSTFYLLYRIISAATFVVSRRIDMVGDQ
                     FSSRIAGSSEFRSTQFRLRSLHYGQQHANRELLKSWRNSRLSSNFPCLVVDHADTLQL
                     SLRPRLIQEMEELVTPLTRSRIVDLGRIVNVEHYQDEGACFLLGPAISLLRGADGIAR
                     TVSIGHYHALGIREPELMDTQRLPVPVPGERDKANRTDVFAGLERTGRVMRIDDFDIH
                     VARPVDERCGELEDLIDKIHHDAAVIHHLADTYRNFEYR"
     gene            complement(1741..2388)
                     /locus_tag="VFX11_11480"
     CDS             complement(1741..2388)
                     /locus_tag="VFX11_11480"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4939288.1"
                     /translation="MIPSPELPFVLDGVRIEPRRPPLLPEIELLLMTDQYPDQALAGP
                     DYHRLMAQPPYWAFCWGGGQALARWILDRPEQVAGRHVIDFGSGSGVAGIAAALAGAS
                     AVVAVDIDPDALRMSARNARANGMAVDTAISCNACHDSLLLAADICYEEAGYAAVLAH
                     IAAGGAAIIAESRLRDLHLRHPHLQPVAQFQVRTFPDLEESENFDLVRIFSTITV"
     gene            complement(2483..2920)
                     /locus_tag="VFX11_11485"
     CDS             complement(2483..2920)
                     /locus_tag="VFX11_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4939289.1"
                     /translation="MVEKTVTVRDCMSRKVFSFDLSDNIAHAVSQLLEHRITGAPVLD
                     AEHNVIGFVSEQDCIKEMLNTAFYCDLTATVGDVMQRDVLSVEPDLPIAQLAEQLTIA
                     KPRVYPVVEAGRLVGIISRSDVLQALFDANVRYHHLSSARAAI"
     gene            3121..4347
                     /locus_tag="VFX11_11490"
     CDS             3121..4347
                     /locus_tag="VFX11_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX4939290.1"
                     /translation="MAKSKAQKPSSRPFHITLFGATGFTGRLTAEYLAGCQDSSHLKW
                     AIAGRNPAKLEECRKAILALNPQADVSIIEADTRDPASLKKMAEQSKVIITTVGPYLN
                     YGEPLVRACVEAGTHYVDLTGEPEFVESLEYEFQDAAQQKAVKIVNSCGFDSIPHDLG
                     ALYTVHELNKLLGAERAGKTPVRVEGFVQAGGTFSGGTWHSAITQFSRLREYAKKRKV
                     WHQARSARPTDRRRSRVASPDIKYCKPFGAWAFPFPTIDPQVVKRTGAARKEYGPDFC
                     YGHYVLTKHLYTGLGGAVAVGGLVALSQFEFGKQKLLKVKDPGQGPSPSQREKSWFRV
                     RFVGEAAGLHVWTEVSGGDPGYGETSKMLAESALCLAFDDEKLPANYGVVTPGAAMGE
                     ALINRLQNAGIRFRTI"
BASE COUNT          967 a         1282 c         1331 g          782 t
ORIGIN      
        1 cggtattcga aattgcggta ggtatcagcc agatgatgga tcaccgccgc atcgtggtga
       61 atcttgtcga tcaggtcttc cagttcgcca caacgctcgt cgaccggccg ggcaacatga
      121 atgtcgaaat cgtcgatacg catcacgcgc cccgttcgtt ccaggcccgc aaatacatcg
      181 gtgcgattgg ccttgtcccg ctcaccgggc acgggcacgg gcagacgctg ggtgtccatc
      241 agttccggct cgcggattcc cagcgcatgg taatggccga tcgaaacagt acgggcgatg
      301 ccgtcggcac cgcgcagcag ggaaatggcc ggccccagca ggaagcaggc gccttcgtcc
      361 tgatagtgtt cgacgttgac gatacgcccc agatcgacga tgcggctacg ggtcagcggc
      421 gtcaccagtt cttccatttc ctggatcagg cgcggccgca acgacaactg cagggtatcg
      481 gcgtggtcta ccaccagaca ggggaaattg ctggaaagtc ggctgttgcg ccagctcttg
      541 agcaactcgc ggttggcatg ctgctggccg taatgcagcg agcgcaggcg gaactgggtg
      601 gagcggaatt cactggaacc ggcaatgcgg gaggaaaact ggtcgcccac catgtcgatg
      661 cgccggctca ccacaaaggt ggctgccgaa atgatgcgat acagcaggta aaacgtgctc
      721 tgtaccagga aatcgaaggg ccgcaacgaa gcggccagac gctggcggga gcggccttca
      781 tccagggaac ggaattcttc cttgatggag gtggcggaac gggccaggcc agactgggcg
      841 aacagccagc ggtcgatggc gcagatcagc gggtagccgt aacggcgtgc ttcgctggta
      901 tagccggcat aggcatgggc aatgacacct gccagttgcc gcgcactcag gccatacagc
      961 aaaggaacac cgatcaggat ttcaggcccg tgtccgagca ggatgtcctt gagagaacct
     1021 ggccgcagga tcagacccac ttcagcatcc agtttgatgg cacggggaaa gggaacccgc
     1081 agcagcagcg ccacatggtt cacaaaggca tgcagttcag gttgagactg gacggaaacc
     1141 gccagatatt cgcgggaatg gcggcggatc agcaccaggc gccgcaccat ggccgccagg
     1201 aatcccacca gaaacagatc gcccagcacc acgacaacag cccagcccgg ccggctggcg
     1261 aatccgttca gcagcaaatc acgggtgaac tggaaccaga ccagacagga aagcccgatc
     1321 agggtgacga acgccagcgg caacgtaatg gcgaacaggg tagccagggc catttttacc
     1381 cgttccccca gggcagtcct gggcgagcgg atttccgccc gaaacccgtt cctgatgtcg
     1441 tcctggctgt aggtgtcgct caacattcgg tctcgcacag ccgtgtcgcg gtatcccgag
     1501 ttgagcaacc ccatagcacc aggaaaattg tgcgatgctt cccgcaacat gatgaggtta
     1561 tctccgctct gaaggaatcg cgaatccggc tgaaccggtc aaagtggatg gtctggaaaa
     1621 ctggtcaaat caaagtcaga tgcactatag cagagggtta caacttaata agtgagcatc
     1681 cgtaggggat tgtgttgagt ttttgttttg ctttgtaacg actggaattt caaggttttt
     1741 tcaaaccgtg atcgtcgaaa aaatgcgcac cagatcaaaa ttttcggatt cttccagatc
     1801 gggaaaggta cgcacctgaa actgcgccac cggctgcaga tgaggatgcc gcaggtgcag
     1861 gtcgcgcagc cgggattccg cgatgatggc ggcgccaccg gcggcaatat gggccagaac
     1921 ggccgcatag ccggcttcct cgtagcagat gtccgccgcc agcagcaggc tgtcgtggca
     1981 ggcattgcag ctgatggcgg tgtccaccgc catcccgttg gcccgggcgt tacgcgcact
     2041 catccgcagt gcgtccgggt cgatatccac cgcgaccaca gcggaggctc ccgccagtgc
     2101 cgccgcaatg ccggccaccc ctgaaccgga gccaaaatcg ataacgtgcc gaccagccac
     2161 ctgctccgga cggtccagaa tccaacgcgc cagcgcctgt ccgccgcccc agcaaaacgc
     2221 ccagtaaggc ggctgcgcca tcagccggtg ataatccggg ccggccaggg cctgatcggg
     2281 atactggtct gtcatcagca gcagctcgat ttcaggcagc aggggcggac ggcggggctc
     2341 gatgcgcacg ccgtccagca caaagggaag ttcgggggat gggatcatga cgggaattcg
     2401 caccagtgtc ggacaggggc gccaccttgc cagccggcct gtccgctggc aagcctgcag
     2461 caacacgcat gcaacctgca gatcagatgg cggcccgggc cgaactcagg tggtgataac
     2521 ggacattggc gtcaaacagg gcctgcagta catcggagcg gctgatgatt cccaccagcc
     2581 ggcctgcctc gaccaccgga tagaccctgg gcttggcaat ggtcagctgc tcggccagct
     2641 gggcgatggg caggtcaggc tcaaccgaga gaacgtcacg ctgcatgaca tcgcccacgg
     2701 tcgcggtaag gtcacagtaa aaagcggtat tgagcatctc cttgatgcaa tcctgctctg
     2761 agacgaaacc gatgacgtta tgttcggcgt ccagcaccgg cgcgcccgtg atgcgatgtt
     2821 ccagcagctg cgacaccgcg tgggcaatat tgtcggacag atcgaaactg aaaaccttgc
     2881 ggctcatgca atcccgcact gtaactgtct tctccaccat aaaccctccg gatttgcgtg
     2941 acgggttggc gcggtacaga cagatccgcc tgcatctacg atggcacagg ggcaggacaa
     3001 gctcaactgt cggtctcaaa ctgctattct caactgcaga accggttgaa agcgataatt
     3061 catccggctt aatctagcac ctcaaatttc agtaacttat caggcttaca ggggaacaca
     3121 atggccaagt ccaaggcgca gaaaccgagt tctcgccctt ttcacatcac cttgtttggc
     3181 gccaccggct ttaccggacg cctgacggcc gaatacctgg caggttgcca ggacagctcg
     3241 cacctgaaat gggccatcgc cggccgcaat cccgccaaac tggaagaatg ccgcaaggca
     3301 attctggcgc tgaatcccca ggcggacgtc agcatcatcg aggccgatac ccgcgatccg
     3361 gccagcctga aaaaaatggc tgagcagagc aaggtgatca tcaccaccgt tggcccctat
     3421 ctgaattatg gcgagccact ggtgcgggcc tgcgtggaag caggtaccca ttatgtggac
     3481 ctgaccggcg aaccggagtt cgtcgaaagc ctggagtacg agtttcagga tgcggcccag
     3541 caaaaagcgg tgaaaatcgt caacagctgt ggtttcgaca gcattcccca cgatctgggc
     3601 gcactctaca cggtgcatga gctgaacaaa ctgctgggcg ccgagcgtgc cggcaaaacg
     3661 ccggtacgcg tggaaggttt cgtgcaggct ggcggcactt tttccggcgg cacctggcat
     3721 tcggccatca cgcagttttc ccgcctgcgc gagtacgcga agaagcgaaa agtgtggcac
     3781 caggcccgca gcgcccgtcc caccgaccgc cgccgttccc gcgtggcgtc gccggatatc
     3841 aaatactgca aaccgttcgg tgcctgggcg ttccccttcc ccaccatcga tccccaggtg
     3901 gtgaaacgca cgggcgcggc acgcaaggaa tacgggccag acttctgcta cggccattat
     3961 gtgctgacca agcatctgta cacgggtctg ggcggtgccg tggcggtggg tggtctggtg
     4021 gcactgtcgc aatttgaatt cggcaagcag aagctgctca aggtgaagga tccgggccag
     4081 ggccccagtc cgtcacaacg ggaaaagagc tggttccggg ttcgctttgt gggggaagca
     4141 gccggtctgc atgtgtggac cgaagtgagt ggcggcgatc cgggttatgg cgagacatcg
     4201 aaaatgctgg cggaatcggc actgtgtctg gcctttgacg acgaaaagct gccggcaaac
     4261 tacggcgtgg tgacaccggg tgccgccatg ggggaggcgc tgatcaaccg tctacaaaat
     4321 gcgggaatcc gcttccgcac catttgattt tttgtttttt ca
//
LOCUS       DASUEG010000610         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1795417, whole genome shotgun sequence.
ACCESSION   DASUEG010000610 DASUEG010000000
VERSION     DASUEG010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795417"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VFX11_11495"
     CDS             complement(<1..1563)
                     /locus_tag="VFX11_11495"
                     /inference="COORDINATES: protein motif:HMM:NF039329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939291.1"
                     /translation="MTEGPTTQTMKFVPITLQERQGIHRTAEPLRVGVPLAEGVVASA
                     SHLQLQDEDGSAIPADIQVTARWADSGLRWCLLDFSASVPALGVKKLQLAVLETAPAA
                     APPAALSLQEQDGGFVVATGVARFRIAASQPALSLLTASGDGIALHFPQLNWPQLITA
                     NGDSCTVQVEQTDVSARQAQARLSVSQQGVFAAPAQAPANEPFCRFESRFTFFANSTR
                     VHWEFTLHNPRAALHPGGLWDLGDPGSVFFRQLSAGIHLPSPGSTHYQLEAGQDWTTA
                     MANAELLQASSGGEHWDSRNHVNHQGQVTLPFRGYRFGDGVREQSGLRASPLVFSSAG
                     VSLHPVQFWQNFPKAITATPESIKLQLFPEQAGDLFELQGGEKKTHSVMFDFSADRDA
                     LAGARAPLAVTLPADYIASTGCIQHFAAQPEPLDDLIATGLDPARGFSAKREIIDEYG
                     WRHFGDIFADHESLYLKNGELFISHYNNQYDPLYGFIRQFLHTGNPAWMELANDLARH
                     VTDIDIYHTQADR"
     gene            1709..2926
                     /locus_tag="VFX11_11500"
     CDS             1709..2926
                     /locus_tag="VFX11_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX4939292.1"
                     /translation="MDIVFLSHRVPFPPNKGEKIRTFHQIKFLAQRGHRIQVLAPVAG
                     EEDIRHMASLKQDYCQQTFHAPQPGPLSYLRGLLTGQALSVSHFYSPALQRQLDQLLA
                     AQKVDAIVCTSSSMAEYVFRSEPLRRAGNQRPALVMDFMDLDSDKWRQYQKLKSFPMT
                     LVYGREAKLIARYEQKIHQAFDACLFISQNEVDLFMQNNPELGRVHVVANGMDTETFR
                     PAAGPKPQQGPNFLFTGVMDYLPNEDAVVWFVEGAWEQVRARYPDAQFFIAGMNPSPK
                     VKALSRFPGIVVTGFVDDIREYFDKAHIFIAPFRLARGVQNKVLQAFGCALPTITTAM
                     GCEGINCKPGEHVLVADTLEQTLAQIDWVMTHPEEAARIGRNAHDLMQREYSWNGKLA
                     ALESILSTRKAAG"
BASE COUNT          590 a          893 c          959 g          637 t
ORIGIN      
        1 gcgatctgct tgcgtgtggt agatgtcgat gtcggtgaca tgacgggcca gatcgttcgc
       61 cagctccatc caggcaggat tgccggtgtg caggaactgc cggatgaagc catacagtgg
      121 atcgtactgg ttgttgtagt gcgatatgaa cagctcgccg ttcttcaggt acaggctttc
      181 atgatccgca aaaatatcgc cgaaatgacg ccagccgtat tcgtcgatga tttcccgctt
      241 ggcggaaaat ccgcgcgccg gatcaagccc ggtggcgatc aggtcgtcga gtggttccgg
      301 ctgcgcggcg aaatgctgga tgcaaccggt gctggcaatg tagtccgccg gtaaggtgac
      361 ggccagtggc gcgcgggcgc cagcgagggc atcgcgatcg gcgctgaaat caaacattac
      421 gctgtgggtt ttcttctcgc cgccctgcaa ttcaaacaga tcgccggcct gttccggaaa
      481 taattgcagc ttgatggatt ccggtgttgc cgtgatggcc ttgggaaaat tctgccagaa
      541 ctggaccgga tgaaggctca cgccggcgct gctgaacacc agcgggctgg cacgcaagcc
      601 ggactgctcg cgaacgccgt caccgaagcg ataaccgcga aagggcagcg tcacctggcc
      661 ctggtggttg acgtggttgc ggctgtccca gtgttcgccg ccgctggagg cctgcagcaa
      721 ttcggcgttg gccattgcgg tagtccagtc ctggcctgct tccagctggt agtgcgtcga
      781 acccggtgac ggcaggtgaa tgccggcgct cagctggcgg aaaaacaccg agcccggatc
      841 acccagatcc cacagaccac ccggatgcag cgcggcgcgc ggattgtgca gcgtgaattc
      901 ccagtgtacg cgggtgctgt tggcaaaaaa ggtgaagcgg gattcgaagc ggcagaaggg
      961 ttcgttggct ggtgcctgtg caggtgccgc gaaaactccc tgttgcgata cggacagcct
     1021 tgcctgggcc tgccgtgcgg aaacgtccgt ttgttcgacc tgcacggtgc agctgtcacc
     1081 attggccgtg atcagctgcg gccaattcag ctgtggaaag tggagtgcga tgccatcgcc
     1141 gcttgcagtc aacagactga gagccggttg gctggccgca atccggaatc gggctacacc
     1201 ggtggcgacg acgaaaccgc catcctgttc ctgcaggctc aatgcagctg gcggtgctgc
     1261 ggctggcgcc gtttccagaa cggcaagttg cagttttttg acacccaggg cgggcaccga
     1321 cgcactgaaa tccagcaggc accagcgcag gccgctgtcg gcccagcggg cagtgacctg
     1381 aatgtccgcc ggaatggcgc tgccgtcctc atcctgcaat tgcaggtgac tggcgctggc
     1441 cacaacgcct tccgccagcg gcacgcccac gcgcagcggc tcggccgtcc ggtggattcc
     1501 ctgtcgttcc tgcagtgtga tcgggacaaa cttcatggtc tgcgtcgtcg ggccttcggt
     1561 catgtgggtg agcctggggg attttgccgg gtgcttatat taaagcaaat gcgcgcattg
     1621 aagcaggggt tccggcagta ctatgctttt aatgtcgggt tgaagttggc aaactacccc
     1681 ggactttcat ccaccaccct ggccctgcat ggacatcgta ttcctatccc accgggttcc
     1741 cttccccccc aacaagggtg aaaagattcg cacctttcac cagatcaagt ttctggcgca
     1801 gcggggccat cgcatccagg tactggcgcc ggtggcaggc gaggaggaca tccggcacat
     1861 ggccagtctg aagcaagact attgccaaca gaccttccac gcgccgcaac ctggcccgct
     1921 gtcctacctg cggggacttt tgaccggtca ggcactcagt gtcagccatt tttattcacc
     1981 ggcgttgcag cgtcagcttg accagttgct tgcagcacag aaggtggacg ccattgtctg
     2041 caccagttcc agcatggcgg aatatgtgtt tcgttcggag cccttgcgcc gtgccggcaa
     2101 ccagcgtccc gcgctggtga tggatttcat ggacctggac tcggacaagt ggcggcagta
     2161 tcagaagctg aaatccttcc ccatgaccct ggtctatggt cgcgaggcga aactgatcgc
     2221 gcgctatgaa cagaaaatcc accaggcctt cgatgcctgc ctgttcatct cgcagaatga
     2281 agtggacctg ttcatgcaga acaatccgga gctgggccgg gtgcatgtgg tggccaacgg
     2341 catggacacc gaaaccttcc gcccggccgc cggacccaag ccgcagcagg gcccgaattt
     2401 cctgtttacc ggcgtaatgg attacctgcc caacgaggat gcagtggtct ggtttgtcga
     2461 gggcgcctgg gaacaggtgc gggccagata cccggacgcg cagtttttta ttgccggtat
     2521 gaatccgtcg cccaaggtga aagcgctgag ccgatttccc ggcatcgtcg tcaccggatt
     2581 tgtcgatgac atccgcgaat atttcgacaa ggcgcatatt tttatcgcac cgttccggct
     2641 ggcgcgcggc gtgcagaaca aggtgctgca ggcgttcggt tgcgcgttgc ccaccatcac
     2701 taccgcgatg ggttgcgagg ggatcaactg caaacccggc gagcacgtgc tggtggcaga
     2761 cacgctggaa cagacgctgg cgcaaatcga ctgggtcatg acgcatccgg aagaggccgc
     2821 gcgcatcggc cgcaatgccc acgatctcat gcagcgcgaa tattcctgga atggcaaact
     2881 ggcggcgctg gaatcgattc tgtccacgcg gaaagcggcg gggtaatttg ctgtgcgcac
     2941 tcacattaaa aatgcgattt atctgctgtt tgtggtgctg gccagtccgg tgtttttgtc
     3001 ttaccggttg ctggcgctga tcggcaaccg cgacagcctg accgccggtt attcccagtt
     3061 tctcagcctg attccgggc
//
LOCUS       DASUEG010000611         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1924872, whole genome shotgun sequence.
ACCESSION   DASUEG010000611 DASUEG010000000
VERSION     DASUEG010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924872"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFX11_11505"
     CDS             complement(<1..405)
                     /locus_tag="VFX11_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX4939293.1"
                     /translation="MKFSIFQESRVGRRKYNQDRVGHAYTRDSLLMVVADGMGGHLHG
                     EVASQITVELFVQHFQQKATPTLKSPWQFLKDSSLRAHDAILTYAARNHMIESPRTTV
                     VAAIIQDGLVYWAHVGDSRLYLFRQQQCVAITR"
     gene            complement(402..1370)
                     /locus_tag="VFX11_11510"
     CDS             complement(402..1370)
                     /locus_tag="VFX11_11510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4939294.1"
                     /translation="MPKQNNKPLPRGYQLLNYTIVKQLSAGGFSIVYLAHDEHDYPVA
                     IKEYLPSTLVLRTEGDMIIATSEEKLATFRYGLKCFFEEGKTLAHISHNNVVRVLNFF
                     RANETVYMVMEYERGRTLQKEIQLHPEGVREELIRHVFYQLLNGLREVHLRKMLHLDI
                     KPANIYLRRDGTPVLLDFGSARRMLGPDRPRFTPMYTPGFAPPEQYKDRDNLGPWSDI
                     YSIGASMFACLAGYAPQAADQRLEHDRHISTRKVWAGKYSDQLLNIIDWCLQLNYLER
                     PQSVHALQKALLTPAGDPPSKPSLMVNLRSTLTGLANLDIRELFRR"
     gene            1629..2501
                     /locus_tag="VFX11_11515"
     CDS             1629..2501
                     /locus_tag="VFX11_11515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX4939295.1"
                     /translation="MSLLSMTGFASISREVPGALLSLDLRAVNHRYLDLQLRMPEDWR
                     SLEGPMRERLQGKLTRGKVECRLSVQQTAQPNAELNLGWSLVEQLLQADRRIRELSPD
                     SPPLSVGEVLRWPQVVQAQAGLSLEEQQNLVLGMLDEALGEFAASRAREGEKLGLYLL
                     ERVERMEQLVREIAPRIPQIVAAQQEKLLTRLRDALGKEDSERLHQEVVLFAQKIDVD
                     EELGRLNAHLAEVRRIVKTGGTVGKRLDFLMQELNREANTLGSKSASVDTTGTAVEFK
                     VLIEQMREQVQNIE"
     gene            2615..3232
                     /gene="gmk"
                     /locus_tag="VFX11_11520"
     CDS             2615..3232
                     /gene="gmk"
                     /locus_tag="VFX11_11520"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX4939296.1"
                     /translation="MATGNIYIVTAPSGAGKTTLVANLLKADPQVQLSVSYTTRAPRE
                     GEVEGRHYHFVSRDSFLEMANNGEFLESAEVYGNFYGTSQRWISEQLRAGRDILLEID
                     WQGAQQVRRIFPDAIGIFILPPSLETLRERLIGRGKDSAEVIQRRLDHAREDIQHVDE
                     FDYIIVNNDLTEATQDLVALVRGRRLRIETQMKRHAELVSALKHG"
     gene            3300..3506
                     /gene="rpoZ"
                     /locus_tag="VFX11_11525"
     CDS             3300..3506
                     /gene="rpoZ"
                     /locus_tag="VFX11_11525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX4939297.1"
                     /translation="MARITVEDCLKKINNRFDLTLAATYRARQLANGSAPMVEAGRDK
                     PTVVALREVAQGLVGREILNRGRA"
BASE COUNT          703 a         1028 c         1151 g          734 t
ORIGIN      
        1 gcgcgtaatg gcaacgcact gctgctggcg gaacaggtac aggcgcgagt cacccacatg
       61 agcccagtac accaggccat cctggataat ggcggcgacc acggtggtgc gcggcgactc
      121 gatcatgtga ttgcgcgcgg cataggtcag aatggcatcg tgggcgcgca gcgagctgtc
      181 tttcaggaat tgccacgggc ttttcagcgt gggggtggcc ttttgctgaa agtgctgcac
      241 aaacagctct acggtaatct gcgatgccac ttcgccatgc aggtgcccgc ccatgccatc
      301 ggcgaccacc atcagcagcg agtcacgcgt gtaggcgtgt cccacccggt cctgattgta
      361 tttgcgccgg cctacccggc tttcctgaaa gattgaaaat ttcatcggcg gaacaattct
      421 ctaatgtcca gattggccag accggtcagc gtgctgcgca ggttgaccat gagtgacggt
      481 ttgctcggcg gatcgccggc cggggtcagc agggcttttt gcagggcatg cacgctctgc
      541 ggccgctcca gataattcag ttgcaggcac cagtcgataa tgttgagcag ctggtcggaa
      601 tatttgccgg cccacacctt gcgcgtcgaa atatggcggt catgctccag ccgctggtcg
      661 gccgcctgcg gggcgtagcc ggccagacag gcgaacatgg acgcgccgat gctgtagatg
      721 tcgctccagg ggccgaggtt gtcgcgatcc ttgtactgct cgggcggggc aaagcccggc
      781 gtgtacatgg gggtaaagcg cggacggtcc ggtccgagca tgcggcgggc ggagccgaag
      841 tccagcagta ccggcgtgcc atcgcggcgc agataaatat tggcgggctt gatgtccaga
      901 tgcagcatct tgcgcaggtg cacttcgcgc aggccgttga gcaactggta gaacacgtgg
      961 cggatcagct cttcccgcac gccttccggg tgcagctgga tttccttttg cagggtacgg
     1021 ccacgttcgt actccatgac catgtacacc gtttcattgg cgcggaaaaa attgagcacc
     1081 cgcacgacat tgttgtgcga gatgtgcgcc agggttttgc cttcttcaaa gaaacatttc
     1141 aggccgtagc ggaaggtggc cagcttttct tccgaggtgg caatgatcat gtcgccttcg
     1201 gtgcgcaaca ccagggtgct gggcaggtac tccttgatgg cgaccgggta atcgtgttcg
     1261 tcgtgcgcca gatagacgat actgaacccg ccagcggaca gttgcttgac gatggtgtaa
     1321 ttgagcaatt gataaccgcg tggcagcggc ttgttatttt gttttggcat tctttgcgat
     1381 aatcacacgt ccaggtgagg ctggaggtcc gttgttcacc agccgtagcg ctgatgggcg
     1441 cggctctgtc tcattcgatt gtgggcacag attcttctaa agtaaaggaa accacgaaca
     1501 agccgccgcg ccttcatcgg gcctgaaccc ggctgacggc ggcccttcgg aggtattccg
     1561 tacctggcgt ccaccccttt tgatcttatg cggtttgtgc ctgcgggcac gatccggctg
     1621 gagaccttat gagtttgttg agcatgactg gctttgccag catcagtcgt gaagtacccg
     1681 gagccctgct gtcgctggat ttgcgagcgg tcaatcatcg ctatctggat ttgcagctgc
     1741 gcatgccgga agactggcgc agcctggaag ggccgatgcg tgaacgcctg cagggcaagc
     1801 tgacccgcgg caaggtggag tgccgcctgt cggtgcagca gacggcgcag ccgaatgccg
     1861 aactgaatct gggctggtcg ctggtcgagc agttgttgca ggccgaccgg cgcattcgtg
     1921 agctgtcgcc ggacagtccg cccctgtcgg tgggcgaggt gctgcgctgg ccgcaggtgg
     1981 tgcaggccca ggccgggctg tcgctggaag aacagcaaaa cctggtgctg ggcatgctcg
     2041 acgaagcgct gggcgagttt gccgcgtcgc gcgcccgtga gggagaaaag ctgggccttt
     2101 atctgctgga gcgcgtcgag cgcatggaac agctggtgcg tgaaatagct ccgcgcattc
     2161 cgcagatcgt ggccgcccag caggaaaagc tgctgacgcg actgcgcgat gccctgggca
     2221 aggaagacag cgagcgcctg catcaggagg tggtgctgtt tgcccagaag attgatgtgg
     2281 acgaggagct ggggcgcctg aacgcgcatc tggcggaagt gcgccgcatc gtcaagaccg
     2341 gaggcacggt aggcaagcgc ctggatttcc tgatgcagga actcaaccgc gaagccaaca
     2401 cgctcggttc caaatcggcc agtgtggata ccaccggcac agccgtcgaa ttcaaggtgc
     2461 tgatcgagca gatgcgcgag caggtacaaa atatcgaata aatgtcgggg ccggggtttt
     2521 ttactgattc cggccacccg tctcactggc ctatactggc aggtgcaggt cagttgcacg
     2581 caaaaagccc gttaattcag tactctggaa agttatggcg accggaaaca tctatatcgt
     2641 gacggctcct tcgggggccg gcaaaactac cctggtggca aatctgctca aggccgatcc
     2701 gcaggtgcaa ctgtcggtgt cgtataccac ccgcgccccg cgcgagggcg aggtcgaagg
     2761 ccggcactat cactttgtca gccgcgattc cttcctggaa atggccaata acggcgagtt
     2821 tctcgagagc gccgaggtgt atggcaattt ttacggcacc tcgcagcgct ggatcagcga
     2881 gcagttgcgc gccgggcgcg acatcctgct ggaaatcgac tggcaaggcg cgcagcaggt
     2941 gcggcgcatt tttccggatg ctattggtat cttcattctg ccgccgtcgc tggaaaccct
     3001 gcgcgagcgc ctgattggtc gcggcaagga cagcgccgag gtcattcaac gccggctcga
     3061 ccatgcgcgc gaagacattc agcatgtgga cgaattcgat tacatcatcg tcaacaatga
     3121 tctgaccgag gccacgcaag acctggtggc actggtgcgc ggccgccgcc tgcgcatcga
     3181 aacccagatg aagcgccacg cggaactggt ctccgcgctc aagcacggat aaactggcgt
     3241 tattgcatca gttttacgtt tttgtgtcgt atccgtttat cagtaaagga agcataacca
     3301 tggcccgtat taccgttgaa gactgcctga agaagatcaa caaccgcttt gatctgaccc
     3361 tggccgccac ctaccgcgcc cgtcagctgg ccaacggttc cgccccgatg gtcgaggctg
     3421 gccgtgacaa gccgaccgta gtggccctgc gtgaagtggc ccagggtctg gttggccgcg
     3481 agattctcaa ccgcggtcgc gcctgagccc gctggccacc ggtcgttaac gacggtggcc
     3541 gtccggatgt acccgccgat ggaagctccc gcgctcgcaa ccgcaccatc gacgatcagc
     3601 agtgctgatg ttgcag
//
LOCUS       DASUEG010000612         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2079656, whole genome shotgun sequence.
ACCESSION   DASUEG010000612 DASUEG010000000
VERSION     DASUEG010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079656"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3193)
                     /locus_tag="VFX11_11530"
     CDS             complement(<1..>3193)
                     /locus_tag="VFX11_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4939298.1"
                     /translation="RDARQTADKFFPDPFGAPGEKLYRTGDLARFLPDGEIEYLGRID
                     TQVKIRGFRIEPGEIEATLRQLSTVKACAVIAREDQPGRKELVAYVVFQAGELSSNGL
                     SSNDLRAHLKKQLPEFMVPAHFVTMDALPLTPNGKVARSLLPAPNRDNASDRVLVEPS
                     TATEKTVLAIWQGVLGTDVISVEDDFFAVGGHSLLATQVVSRLRREFRIDLPLRSLFE
                     APTVRHTAAVIDRVLLDSTQNQLPPVVRIDRAGKLPLSFVQQQLWLLDQLDPGTPAYN
                     MPVALRIAGALDVAAFEQAFRHVIARHETLRTNFIVQNGEPVAVISGDTQWHFDQVDL
                     SSLDALEQDARVQAIATQQTEQGFNLAQDCLIRGSLIRLSNDESGKSRFVFVGAIHHI
                     VSDGWSLNIMIAELMEFYRAGLQNRAALLPELDVQYVDVAAWQRQWLQGEVLESHIAF
                     WRDQLDNEHQVLNLPTDFPRPPVMTSNGASLGSLLPASVVSGAQQLAREEGATLFMVL
                     LAAYQLLLGRYAGQERVNVGTPIAGRDSVETENLVGFFINTVVMSTDVSGDLTFRTLL
                     RRVREVALGAYAHQALPFEKIIEELKPRRDTSRTPFFQVFLNLLNLPPQADAQADLLI
                     EPLLREDNHAHSKYDLNLYASETGTELELLMVYNRDLFNANTVERLLADLSLLMERAL
                     AQPDEPLARISTRSDRDQAWLPALDQPIAKSAFVSPVERFLQQATQQPDAIAIRAAGQ
                     TLRYRELEAASATISAQLQAAGVGEGDAVAILAERSPALVASLLGVLRAGAVFCVLDT
                     AYPVERLVGYVRLLQPKALLIATAQDNPVLTALAPVLDESGCRQQWHVADALAGKAVQ
                     RAAERHDLQRRAYVAFTSGTTGVPKGIAGRFEPVAHFVDWYIGEYAVTAQDRFSLLSG
                     LAHDPLLRDIFVPLAAGAQISIPAANQLTDSTALMHWFAQEKITCTHLTPAMCRVLTA
                     GSSSMQIPDLRLAGFGGDRLTLDVVVALQAIAPAAQAVNFYGTTETPQVMAVYPVPSA
                     QTVAGGTLPIGRGIEGVQILVLDEKLHLCAPGCV"
BASE COUNT          677 a         1048 c          911 g          557 t
ORIGIN      
        1 cacgcaaccg ggcgcgcaca gatgcagttt ttcgtccagc accagaatct gcacgccctc
       61 gatgccacga ccgataggca aagtgccgcc cgcaaccgtt tgcgcggacg gaaccggata
      121 caccgccatg acctgcggcg tttccgtggt gccgtaaaaa ttcaccgcct gcgctgcggg
      181 cgcaattgcc tgtagtgcta ccaccacatc cagcgtcaga cggtcaccgc caaaacctgc
      241 cagacgcaga tccgggattt gcatcgacga cgaaccggcc gtcagcacgc gacacatcgc
      301 gggcgtcagg tgcgtgcagg taattttttc ctgcgcaaac cagtgcatta gcgcggtgga
      361 atccgtcagt tgatttgccg ctggaatcga aatctgcgcg cccgccgcca gcggcacaaa
      421 aatgtcccgc aacaacggat cgtgcgcgag tccggacagc agactgaacc ggtcctgcgc
      481 agttaccgcg tattcgccga tataccagtc aacgaaatgc gccaccggtt cgaatcgacc
      541 ggcgatgcct ttgggcacac cggtggtacc ggacgtaaag gcaacatagg cacgccgctg
      601 caaatcgtgg cgctctgctg cacgctgtac cgcttttcca gcgagagcat cggcaacatg
      661 ccattgctga cggcagccgg attcatccag caccggggcc agcgcagtca gcaccggatt
      721 atcctgcgcg gtggcaatca gcagcgcctt cggttgcagc aggcgcacat accccaccag
      781 ccgctcaacc gggtaagcgg tatccagaac gcagaatact gcacctgcac gcaacacgcc
      841 gagcagcgaa gccaccagcg caggcgagcg ctccgccaga atcgcaacgg catccccctc
      901 gcccacacca gctgcctgca gctgtgcgct gatggtagca ctcgccgctt ccagttcccg
      961 gtaacgcagg gtctggccag cggcgcgaat cgcaatggcg tccggctgct gcgtcgcctg
     1021 ctgcaggaaa cgctcgaccg gcgacacgaa cgcagatttc gcaatgggct gatccagtgc
     1081 cggcaaccag gcctgatccc gatcactgcg ggtgctgatg cgcgccagcg gttcatccgg
     1141 ctgggccagc gcccgttcca tcagcaagga aagatctgcc agcagacgct caaccgtatt
     1201 ggcattgaac aaatcgcggt tgtacaccat cagcaattcc agctcggttc ctgtttcact
     1261 ggcgtacaga ttcagatcgt atttggaatg ggcgtgattg tcttcgcgca gcagtggttc
     1321 gatcagcaga tccgcctgcg cgtccgcctg gggcggcagg ttcagcagat tcaggaacac
     1381 ctggaagaac ggcgtgcgtg aagtatcgcg acgcggcttc agttcctcga tgattttttc
     1441 gaacggcagc gcctgatggg cgtacgcacc cagtgccact tcccgcacgc gccgcagcaa
     1501 agtcctgaag gtgagatcgc cgctcacatc ggtggacatc accaccgtgt tgatgaagaa
     1561 gccaacgaga ttttccgttt ccaccgaatc gcgaccggca atgggcgtgc ccacgttgac
     1621 acgctcctgc ccggcataac gacccagcaa caactggtag gccgccagca gcaccatgaa
     1681 cagggtggcg ccctcttccc gtgccagctg ctgtgcgccg gacaccaccg acgccggcaa
     1741 cagcgaaccc agcgaggcac cattgctggt catcaccggc ggacgcggga aatccgtcgg
     1801 caggttcagc acctgatgtt cgttgtccag ctgatcgcgc cagaacgcga tatgggattc
     1861 cagcacctcg ccctgcagcc actgacgctg ccacgcggcc acgtccacat actgcacgtc
     1921 gagctccggc agcaacgccg cacggttttg caggccggca cggtaaaact ccatcagctc
     1981 ggcgatcatg atattcagcg accagccatc agacacgata tggtgaatcg cgccgacaaa
     2041 tacgaatctc gacttgccgg attcgtcatt actcaagcgg atcaggctgc cgcgaatcag
     2101 gcagtcctgc gccagattga agccctgctc tgtctgctgc gtggcaatgg cctgcacccg
     2161 cgcatcctgc tccagcgcat ccagggacga cagatccacc tgatcgaaat gccattgggt
     2221 atcgccgcta atcaccgcga ccggctcgcc attctgcacg atgaaattgg tgcgcagggt
     2281 ttcatggcgc gcgatcacgt gacggaaagc ctgctcaaac gcggccacat ccagcgcgcc
     2341 ggcaatacgc agcgccaccg gcatgttgta cgcgggcgtg ccggggtcaa gctgatccag
     2401 caaccacaat tgctgctgca caaacgacag cggcagcttg ccggcgcggt cgatccgcac
     2461 caccggtggc agctggttct gcgtggaatc cagcaatacg cgatcaatca ccgccgctgt
     2521 gtgtcgcacc gtgggcgctt caaacagcga acgcaacggc agatcgatgc ggaattcacg
     2581 gcgcaggcgg gacaccacct gggttgccag caacgaatgg ccacccacgg caaagaaatc
     2641 atcctcaacc gagatcacat ccgtacccag cacaccttgc cagatcgcca gcactgtctt
     2701 ttctgtcgca gttgacggtt ccaccagcac gcgatcgctg gcattgtccc ggttgggcgc
     2761 tggcagcagc gaacgcgcca ccttgccatt cggtgtcagc ggcagtgcat ccatcgtcac
     2821 gaaatgggcg ggcaccatga attccggcag ctgctttttc agatgcgcgc gcagatcgtt
     2881 tgaggaaagc ccgtttgacg aaagctcacc cgcctggaac acaacataag ccaccagttc
     2941 cttgcgcccc ggctgatcct cgcgggcgat aaccgcgcag gccttgacgg tggacagctg
     3001 acgcaaggtc gcttcgattt cgccgggctc gatacggaaa ccgcgaatct tcacctgggt
     3061 gtcgatccgg cccagatatt cgatttcgcc atcgggcaga aaacgcgcca gatcgccggt
     3121 gcgatacagc ttctcgcctg gcgcaccaaa cggatcgggg aagaatttgt cggcggtctg
     3181 gcgggcatcg cgc
//
LOCUS       DASUEG010000613         9282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1597515, whole genome shotgun sequence.
ACCESSION   DASUEG010000613 DASUEG010000000
VERSION     DASUEG010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597515"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFX11_11535"
     CDS             <1..807
                     /locus_tag="VFX11_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939299.1"
                     /translation="VQALVDDDVEQTFSGIKSKGKGGGGSLGVIALLMLAGLLAARPG
                     KAATAKAASLGAALLVTTAFSLLPAQSGHAAGTEDLQQQILARSYISVSFYQARGSQG
                     SGDFEQGAAADGVVVSLNDYEESRTAWQIATGYRYLDWSAVELGYLDLGETQVDLDAT
                     GTANNLEAAMSDNYPVTGHGWTLANRFLWEAMPRVTLSGEVGLFIWEGEIDLTGANVE
                     PDLDDGTDLLLGVACAYRITDQLEAALHLKRVFFDDQEVDLLGVEGRFHF"
     gene            1033..1365
                     /locus_tag="VFX11_11540"
     CDS             1033..1365
                     /locus_tag="VFX11_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEX4939300.1"
                     /translation="MPATFELKRDADNQFCFHLLDGTGAVLLLSGQYDSKTDAEEAIK
                     EVRVGTLMSTQIAASRTPTGETFFVVRNSHGRIVVKSQLFNNKMLFDNALHQVKDNAC
                     VAEVTDLT"
     gene            1376..1972
                     /locus_tag="VFX11_11545"
     CDS             1376..1972
                     /locus_tag="VFX11_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939301.1"
                     /translation="MGIEFTITKELASISVTATELAAISTLVRGQIGSRDFRGQFDPL
                     LASIAGAYDVVIQLLQPFAALRDAADFNRDFTACHTHYKAVYLKEASRPRLFTEQAFQ
                     QYQILKTCRELQTGFPLLKRTFTRLDELIDKWIDNDCWLVMNMDTVFKLLHRLLNEFE
                     TMRIKDPDEAWLLYRQAFDGFDPYLAILARHRVALAII"
     gene            2183..4234
                     /locus_tag="VFX11_11550"
     CDS             2183..4234
                     /locus_tag="VFX11_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX4939302.1"
                     /translation="MARYLWLIVLTALMLTACGGGSSSGSGDDSAARTQQPVTSDSDT
                     PADDEDDPIVGDETDSPEPPAGAYTRPLDYRYMVTLPLQFLPTADGRLLGVRVTLPAN
                     ERRKPAEGPFPAILVQTAYNMHLIGAVPMTGGALLGAPDPFMVKRGYAEVAVDTLGTG
                     VSQGGWEMLGEDEQKAYGDTVDWVKQQPWFNGKLGVAGASYMAITSLFTAQRRPDDVQ
                     AIFASVPMGDAQRGTVGTGGMINGVFMSHWLTLTQVTTQQNFLAVLQAMNHANLITAA
                     TREHNEQIDNYYLPIIEKALNGDPEVTYDSPFWRTRSPIENIDKIKAPTLIFGALNDI
                     FQRDEPLLYEKLKQNNVDTRLMIYNGDHVGHFVQGFLGVNKLGPMPFLLLQWFDHHLK
                     GIDSGVESIPPVTQYVKNHADHLLNGFSTTTDWPHPEAAPERWYLHGDLSLTQDAPVE
                     EEPVNTMEVAPFATIEYGKSEDGSLLIFNVEPNDGTKCSASYHQWTLGIAGVASFNKC
                     YRDNRELEADALNYETALMEEDYYINGPLQADIWISTTAHEAVLSVRVDEVTANGRTV
                     KPLTNGLLLASARRVDESRSRFMNGEMIQPYHYLTEDAVEPVVPGEVFKMQVEIFPTS
                     AIIRKGHKLRVSIAPSNDAQGMLNLPRQELAKDGVTSIHNSPDYPSSIVLPIVPLSAL
                     N"
     gene            4505..5671
                     /locus_tag="VFX11_11555"
     CDS             4505..5671
                     /locus_tag="VFX11_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939303.1"
                     /translation="MSFELTQDQKNLQATVREWAQAEVAPIAYQLDQAAEFPTELYLK
                     MNREIGIASIPFKEEHGGLGLGILDMSLAIEELARADQTLALTTMVAVATGLTLQSFG
                     SPLLKEKYLPDIVAGKAIGSIAGTEPQAGSWTAGFKSRAEKVDGNKWSISGEKAFITN
                     SGADITSYNLVCAITSPKDSEKTSMTLFLVPAGTPGVTIGKAYDKLGWRSSDTHPIYL
                     DGAVVGDDMIVGKEGDGRYIVHKTYQSARCFLSACALGLSQACLDHSIAYAKDRQAFG
                     RSLGGLQLIQKMIADMAVKVETSRLLARQAAIKADAGTASILELAMAKYYCCEVSSEV
                     ADLALQVHGGWGFMNDCPVSRYLRDTRITRIGDGSSQIQSMIIAKELGLDVTFT"
     gene            5706..6794
                     /locus_tag="VFX11_11560"
     CDS             5706..6794
                     /locus_tag="VFX11_11560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4939304.1"
                     /translation="MSRISGFLQGTKVVDLSRHLPGPLASLLMVDMGARVIKVEPPQG
                     EEMRFIGPVGAQGTSVYFDAVNAGKTGVKLDLKNVDQRAQLLELLTDADVLIESFRPG
                     VLEKLGIGIDFLRQEFPRLVICSLNGYGDHSPLRNDVGHDLNYLAMNGVLAGTGTVEQ
                     TVAPWPPLADCAASLFGLSTILGALLERERSGNGCHVEVALADSVMPLMQFSLAELGL
                     KGRGIPRAEDLLNGGAARYRTYRTADGFSVALGAVEPKFWQAFCHAAGRADWIARMDD
                     PLPQTDLQQELVAFFAALTLQQCNERFGNVDCCFNQVLELHEAVQTPHLQARQLVVQP
                     RPGEFQALYPAYVDGAPPQPRAPFCETH"
     gene            6887..8422
                     /locus_tag="VFX11_11565"
     CDS             6887..8422
                     /locus_tag="VFX11_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4939305.1"
                     /translation="MNIRDMMRRSATFHRDRIAIIAGDHRLTYSEAWERGLRMANLLL
                     SLGLKPGDRVGSLEDNTLEAVDFFLGAAIANIVRVPLYARNARASHMAMLEQTQCKAV
                     VVAEKYADQLEGMNNEIAGLQHIIVRQTNGYEQWLASFAATDPNVAVNDDDLYVIRHT
                     GGTTGKPKGVSFTHKAWLDIGRNWFFTMPPVSPGDVCMHIAPISHASGYQFVPVWLMG
                     GVNLVVEKYQPEAVTQQMVKERVAYVFVAPTMVRDIMAVPGVKDMDWSAMKVILIGAA
                     PITETDVRRAYEVWGNAICQLYGQSEGVPVSVTMASEWMADVPGSQPLRSLGKVHPFC
                     ELEIRDMDTHEPVPFRTEGEICFRVDGQMPGYWQNPESTAKTIIRGFVHTGDVGYLDE
                     NGYLYMCDRKDDMILSGGYNIWPTELENVLASHPAVKEVVAFGVPSERFGESPYAIVT
                     VSDPTTVSEQELIDLCAKELGSYKKPIKVEIRTESLPRTPVGKLSRKLIRDPYWAGLG
                     RKK"
     gene            8453..>9282
                     /locus_tag="VFX11_11570"
     CDS             8453..>9282
                     /locus_tag="VFX11_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009819966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4939306.1"
                     /translation="MSKITSTVNKNSDSYKRNFAYHSELGAQLRTRIEDAINGGRDHL
                     IERHRKRGKLLPRERIDLLVDPCTPFLEFSTLAAYEQYGGKVHSAGVVTGIGVIHGQF
                     CVIVANDSTVKGGAYYPETVKKHIRAQEIAEKHQLPCIYLVDCGGAYLHEWDRVFPDR
                     DHFGNIFYRQCNLSAQGLPQLAAVFGVSTAGAAYIPALSDEVVMVRGNATIHLGGPSI
                     VKVAINEDISPEDLGGAEMHTTVSGVSDYIAETEQEALARMRDIVSKLNRKQGNFQRT
                     "
BASE COUNT         1908 a         2690 c         2874 g         1810 t
ORIGIN      
        1 gtgcaggcgc tggtggatga cgatgtcgag cagaccttca gcggcatcaa gagcaagggc
       61 aagggtggtg gcggttcact gggtgtgatt gcactgttga tgctggcagg tctgttggca
      121 gcacggcctg gcaaggcggc gactgcgaaa gccgcatcgc tgggagcggc cctgctggtc
      181 accacggcgt tcagcctgct gcctgcgcaa tccggccacg ctgccggtac ggaggatctg
      241 caacagcaga tactggcgcg cagctacatc agtgtctcgt tctatcaggc caggggtagc
      301 cagggttccg gtgatttcga gcagggcgcg gcggcggatg gcgtggtggt gagcctgaac
      361 gactatgagg aatcccgcac cgcctggcag attgccacgg gttaccgcta tctggactgg
      421 agtgcggtgg aactggggta tctggatctg ggcgaaaccc aggtcgacct ggatgccacc
      481 ggcacggcca acaatctgga ggcggcgatg tcggataact accctgtcac cggtcacggc
      541 tggaccctgg ccaaccggtt cctgtgggaa gcaatgccgc gcgtgaccct gtcgggtgaa
      601 gtgggcctgt tcatctggga aggtgaaatc gatctgaccg gtgcgaatgt agaaccggac
      661 ctggatgacg gcaccgacct gctgctgggc gtcgcctgcg cttacaggat taccgaccag
      721 ctggaagcgg ccctgcatct gaagcgggtg ttcttcgatg atcaggaagt ggatctgctg
      781 ggagttgaag ggcggtttca tttctgagtt ttttcgccgg tctggtattg cctgccagac
      841 cggcttcccc cctcccgaat tttcccgcgt aacgattccg acaatggcgc cgccctgtca
      901 gtgacggttg cgacaatttt ccccgctaaa tccagcgacg cccttgtcat ttcagcggtt
      961 tgcctctggc gtgcgccttg cttggcgtta cggaactgtt ttttccgttc accagtcacc
     1021 ggggaatctc acatgcctgc aacgttcgaa ctcaagcgcg atgccgataa ccaattctgt
     1081 tttcatctgc tggacggcac cggtgccgtg ctgctgctca gtggccagta cgacagcaag
     1141 accgacgccg aggaggcgat caaggaagtg cgggtgggca cgctgatgag cacgcagatt
     1201 gccgccagcc ggacgcccac cggtgaaacc tttttcgtgg tgcgcaacag ccacggccgc
     1261 atcgttgtga agagccagct gttcaacaac aagatgctgt tcgacaacgc gttgcatcag
     1321 gtgaaagaca acgcctgcgt ggccgaggtg acggacctga cctgaggcgc gcaccatggg
     1381 catcgagttc accatcacca aggaactggc cagcatcagc gtcaccgcca ccgaactggc
     1441 ggccatcagc acgctggtgc gcggccagat cggcagccgg gattttcggg gccagttcga
     1501 tccgctgctg gccagcatcg ccggcgccta tgatgtggtg atccaactgc tgcaaccctt
     1561 cgccgccctg cgcgacgcag cggatttcaa ccgcgatttc acggcctgcc acactcacta
     1621 caaggcggtg tacctgaagg aagccagccg gccccgcctg ttcaccgagc aggccttcca
     1681 gcagtaccag attctgaaaa cctgccgcga actgcagacc ggctttcccc tgctcaaacg
     1741 cactttcacc cggctggacg aactgatcga caagtggatc gacaacgact gctggctggt
     1801 gatgaacatg gacaccgtgt tcaagctgct gcaccgcctg ctcaatgagt ttgaaaccat
     1861 gcgcatcaag gacccggacg aggcctggct gctgtaccgg caggccttcg atggtttcga
     1921 tccctacctg gcgatcctgg cccggcaccg ggtcgccctg gctattatct gatcagctgt
     1981 cgcatccggt aattcaggct ttttctggcc attgccgcct gatttttaag agaaatctgt
     2041 ccgcttaaag ccgttattcc ccctcctcag aggtagtacc ttcgcaccag attagagttg
     2101 acgcactata aacaaactgt ttaacgtgtc gaccaagcca agatcagcgc catcacaata
     2161 acaaacaaaa agagggattt tcgtggccag atacctgtgg ttaatagtgc ttaccgccct
     2221 gatgctgacc gcctgtggcg gagggtcttc ctccggttcc ggcgatgaca gcgccgcacg
     2281 tacccaacaa cccgtgacca gtgacagcga cacgcctgcg gacgatgagg acgatcccat
     2341 cgtgggggac gagaccgatt cccccgaacc gcccgccggt gcctacaccc gcccgctgga
     2401 ctatcgctac atggtcaccc tgccgttgca gttcctgccc accgccgatg gccgtctgct
     2461 gggtgtgcgg gtgaccttgc cggcaaacga gcgtcgcaag ccggccgagg gcccgttccc
     2521 cgcgatcctg gtgcagaccg cctacaacat gcatctgatc ggcgccgtgc cgatgacagg
     2581 cggtgcgctg ctgggtgcgc cggatccctt catggtgaaa cggggctacg cggaagtggc
     2641 ggtggacacg ctgggcaccg gggtgtcgca gggtggctgg gagatgctgg gcgaggatga
     2701 gcagaaagcc tatggcgaca cggtggactg ggtgaagcag caaccctggt tcaacggcaa
     2761 gctgggtgtg gcgggcgcgt cctacatggc gatcacgtcg ctgttcacgg cccagcgccg
     2821 gcccgatgat gtgcaggcga ttttcgccag cgtgccgatg ggcgatgcgc agcggggcac
     2881 cgtaggcacc ggcggcatga tcaacggcgt gttcatgagc cactggctga cgctgaccca
     2941 ggtaaccacg cagcagaatt tcctggcggt gctgcaggcc atgaatcatg ccaacctgat
     3001 tacggcggcc acccgcgaac acaacgagca gatcgacaat tactatctgc ccatcattga
     3061 aaaagccctg aacggcgatc cggaagtgac ctatgacagc ccgttctggc gcacccgttc
     3121 cccgattgaa aatatcgaca aaatcaaggc gcccactctg atcttcggtg ccctgaacga
     3181 cattttccag cgcgacgaac ccttgctgta cgagaaactg aaacagaaca acgtggatac
     3241 ccgcctgatg atttacaacg gcgatcacgt tggtcatttt gtgcagggtt ttctgggcgt
     3301 gaacaaactg ggccccatgc cgttcctgct gctgcagtgg tttgatcacc atctgaaggg
     3361 cattgattcg ggggttgaat ccattccccc ggtcacgcag tacgtgaaaa atcatgccga
     3421 ccacctgctg aacgggtttt ccaccaccac cgactggccg catccggaag ccgcgccgga
     3481 gcgctggtac ctgcacggcg atctgtcgct gacacaggac gcaccagtcg aagaggaacc
     3541 ggtcaacacc atggaggtgg cgccattcgc gacgatcgaa tacggcaaat cggaagacgg
     3601 cagcctgctg attttcaatg tggaacccaa cgacggcacc aaatgttccg ccagttacca
     3661 tcagtggacg ctggggattg ccggtgttgc cagcttcaac aaatgctatc gcgacaaccg
     3721 tgaactggaa gccgatgcgc tgaattatga aaccgcgctg atggaggagg attactacat
     3781 caacggcccg ctgcaggcgg atatctggat cagcaccacg gcgcatgagg ccgtgctgtc
     3841 ggtgcgggtg gatgaagtga cggcgaatgg ccgcacggtg aagccgctga ccaatggtct
     3901 gctgctggcg tcggcgcgca gggtggatga aagccgctcg cgtttcatga atggtgagat
     3961 gatccagccg tatcactacc tgaccgagga tgcggtggag ccggtggtgc ccggcgaggt
     4021 gttcaagatg caggtggaaa tcttccccac cagcgcgatc attcgcaagg gccacaagct
     4081 gcgggtgtcg attgccccca gcaacgacgc ccagggcatg ctgaacctgc cgcgccagga
     4141 actggccaag gacggtgtga ccagcatcca caacagcccg gactatccct ccagtatcgt
     4201 gctgccgatc gtgcccctca gcgcgttgaa ctgacagtgc gttgaactga aacctgccta
     4261 agcggcgtgc cccggcgact gtttgcgccg ggcaggccgc tcccttcact gctccggtca
     4321 acgccccgtc aatgccctcc tgctagtcca cttggacgat gggtgcaccc gctttcccct
     4381 ctcataatcg attcaaccac tggcttgccc gttcggcgtg gcagttcact cgaattgacc
     4441 tgcggcacct ggcgccgggc tgctagattc ccaacgtcaa tacaacaaaa agcggatgac
     4501 tttcatgagc tttgaactga cccaggacca gaaaaacctg caggcgaccg tgcgcgagtg
     4561 ggcccaggcg gaagtggcac cgattgccta tcaattggat caggcggcgg aattccccac
     4621 tgagctgtac ctgaaaatga accgcgaaat cggcattgcg tcgatcccgt tcaaggaaga
     4681 gcacggtggc ctgggactgg gcattctgga catgtcgctg gcgatcgagg aactggcccg
     4741 cgctgaccag accctggcgc tgaccaccat ggtggcggtg gccaccggcc tgacgctgca
     4801 atcctttggc agcccgctgc tgaaagaaaa atacctgccg gacatcgtgg cgggcaaggc
     4861 aattggttcc attgccggca ctgaaccgca agccggttcc tggaccgccg gtttcaaatc
     4921 ccgcgctgaa aaagtggatg gcaataaatg gtccatcagc ggtgaaaaag cctttatcac
     4981 caacagcggc gccgatatca cgtcctacaa tctggtgtgc gccatcacca gcccgaaaga
     5041 ctcggaaaaa accagcatga ccctgttcct ggtgcccgct ggcacaccgg gcgtcaccat
     5101 cggcaaagcc tatgacaagc tgggctggcg ttcgtccgac acccatccga tttatctgga
     5161 tggcgccgtg gtgggcgacg acatgatcgt gggcaaggaa ggcgacggtc gctacatcgt
     5221 gcataaaacc tatcaatccg cgcgctgctt tttgtcggcg tgcgcgctgg gcctgtcgca
     5281 ggcgtgcctg gatcattcca tcgcctacgc gaaagaccgg caggcgtttg gccgctcgct
     5341 gggtggcctg cagctgatcc agaaaatgat cgccgacatg gcggtgaaag tggaaacctc
     5401 ccgcttgctg gcccgtcagg cggccatcaa agccgatgcc ggcactgcca gcattctgga
     5461 actggcgatg gcgaaatact actgctgcga agtgtcctcc gaagtggcgg atctggcgct
     5521 gcaggtgcac ggtggctggg gcttcatgaa cgattgcccg gtatcgcgtt acctgcgtga
     5581 cacgcgcatc acccgcatcg gtgacggttc cagccagatc cagtcgatga tcatcgcgaa
     5641 ggaactgggt ctggatgtga ccttcacctg attttttgcg ttcgtttcag tgagatgtta
     5701 gcgccatgag ccggatcagc ggatttctgc agggaacaaa agtggtggac ctgagccgcc
     5761 acttgcccgg cccactggcg tcgctgctga tggtggacat gggcgcccgc gtgatcaagg
     5821 tggagccgcc ccagggcgag gaaatgcgct ttatcgggcc ggtgggtgcg cagggcacgt
     5881 cggtgtattt cgatgctgtg aatgccggta aaactggcgt gaagctggat ctgaaaaatg
     5941 tggatcagcg cgcgcaattg ctggaactgc tgaccgacgc cgatgtgctg attgaatcct
     6001 tccgccccgg tgtgctggaa aaactgggca tcggcatcga ttttctgcgc caggaatttc
     6061 cgcgtctggt gatctgttcc ctcaatggtt atggcgatca cagcccgctg cgcaatgatg
     6121 tgggccacga tctgaattat ctggcgatga atggtgtgct ggccggcact ggcacggtgg
     6181 aacagaccgt ggcgccctgg ccgccgctgg cagattgcgc ggcgtcgctg ttcgggttga
     6241 gcacgattct gggtgcgttg ctggagcgtg aacgcagtgg caatggctgc catgtggaag
     6301 tggcgctggc ggattcggtg atgccattga tgcagttctc gctggcggaa ctgggtttga
     6361 aagggcgcgg cattccccgc gcggaagatt tgctgaacgg gggtgcggcc cgctatcgca
     6421 cctatcgcac cgccgatggt ttttccgtgg cgctgggggc ggtggaaccg aaattctggc
     6481 aggcgttctg tcatgccgcc ggccgcgccg actggatcgc gcgcatggac gatccattgc
     6541 cgcagaccga tctgcaacag gaactggtgg cattttttgc cgcgctgaca ctgcagcaat
     6601 gcaatgagcg attcggcaac gtggattgct gtttcaatca ggtgctggaa ctgcacgagg
     6661 cggtgcagac gccgcatctg caggcgcggc agctggtggt gcagccacgg ccgggtgagt
     6721 ttcaggcgtt gtatccggcc tatgtggatg gggcgccgcc acagcctcgg gcgccgtttt
     6781 gcgaaaccca ctgagtgaaa aaatcgcagt aacagccgga ccggacaatc cggctgccga
     6841 aggaatcgcc gcagtaaacg ataaaaacaa gtaagaggat ttaagcatga acattcggga
     6901 catgatgcgc cgctccgcca cctttcaccg ggatcgcatt gccatcatcg ccggcgatca
     6961 ccgcctgacc tacagcgaag cctgggagcg cggtttgcgt atggcgaacc tgttgctgtc
     7021 gctgggactg aaaccgggtg atcgggtggg ctcgctggaa gacaacacac tggaagcagt
     7081 ggacttcttt ctgggtgcag cgattgcaaa cattgtgcgc gtgccgctgt atgcacgcaa
     7141 cgcccgggcc agccacatgg cgatgctgga acagacccag tgcaaggccg tggtggtggc
     7201 agaaaaatac gccgaccagc tggaaggcat gaacaatgaa attgccggcc tgcaacacat
     7261 tatcgtgcgg cagaccaacg gctacgaaca atggctggcc agctttgccg ccaccgatcc
     7321 caatgtcgcg gtgaacgacg acgatctgta tgtcatccgt cacaccggcg gcaccaccgg
     7381 caaacccaaa ggggtttcct tcacacacaa agcctggctc gacatcggtc gcaactggtt
     7441 tttcaccatg ccgccggtgt cgcccggcga tgtgtgcatg cacatcgcgc cgatttccca
     7501 tgcatccggt taccagtttg tgccggtgtg gctgatgggc ggcgtgaatc tggtggtgga
     7561 aaaataccag cccgaagccg tgacgcaaca gatggtaaaa gagcgtgtgg cttacgtgtt
     7621 cgtggcgccc accatggtgc gcgatatcat ggcggtgccg ggcgtgaagg acatggactg
     7681 gtcggcgatg aaagtgattc tgatcggcgc cgcgcccatt accgaaaccg atgtgcgccg
     7741 tgcctatgag gtgtggggca acgcgatctg ccagctgtat ggccagtctg aaggtgtgcc
     7801 ggtgtcggtc accatggcca gcgagtggat ggcggatgtg cccggttcgc agccgttgcg
     7861 ttccttgggc aaggtgcatc cgttctgcga actggaaatc cgcgacatgg acacccacga
     7921 gccggtgccg ttccgcaccg aaggcgaaat ctgcttccgc gtcgacgggc aaatgccggg
     7981 ctactggcag aatccggaat ccaccgccaa aaccatcatt cgcggttttg tgcacactgg
     8041 cgatgtcggc tatctggatg agaacggtta cctgtacatg tgcgatcgca aggacgacat
     8101 gatcctgtcc ggcggttaca acatctggcc aaccgagctg gaaaatgtgc tggcctcgca
     8161 tccggcggtg aaagaagtcg tggcattcgg tgtgcccagc gagcgcttcg gcgaatcgcc
     8221 ctatgcgatt gtgaccgtga gcgatccgac cacggtgagc gagcaggaac tgattgacct
     8281 gtgcgcgaag gaactgggtt cctacaaaaa gccgatcaag gtggagatcc gcacggaatc
     8341 cctgccgcgc acaccagtgg gcaagttgag ccgcaagctg atccgtgatc cttactgggc
     8401 tggcctgggt cgcaagaaat aaataggtcg caagaaataa caggaagccg taatgagcaa
     8461 aatcacttcc accgtgaaca aaaacagcga cagttacaaa cgtaacttcg cctatcactc
     8521 cgagctgggt gcacagctgc gcacccgcat cgaagacgcc atcaacggcg ggcgcgacca
     8581 tctgatcgaa cgtcatcgca aacggggcaa gctgttgccc cgcgagcgga ttgatctgct
     8641 ggtggacccc tgcacgccat ttctggaatt ttccaccctg gccgcgtatg agcagtacgg
     8701 tggcaaggtg cacagcgcgg gtgtggtcac cggtattggc gtgatccacg gccagttctg
     8761 cgtgatcgtg gccaacgatt ccacagtgaa aggcggcgcc tactatccgg aaacggtgaa
     8821 gaagcacatc cgcgcccagg agattgccga gaagcaccag ctgccgtgca tttatctggt
     8881 ggattgcggt ggcgcctatc tgcacgagtg ggaccgcgtg tttcccgacc gcgatcactt
     8941 cggcaatatt ttttaccgcc agtgcaatct gtcggcgcag ggtttgccgc agctggcggc
     9001 ggtgtttggc gtcagcacag ccggtgcggc gtatattccc gcgctgtcgg atgaagtggt
     9061 gatggtgcgg ggcaacgcga cgattcacct gggcggtcct tccatcgtga aagtggcgat
     9121 caacgaagac atcagcccgg aagatctggg cggcgcggag atgcatacca ccgtgtccgg
     9181 cgtgtcggat tatattgcgg aaaccgagca ggaagcgctg gcgcggatgc gcgacatcgt
     9241 gtcgaagctg aaccgcaagc agggcaattt ccagcgcacc ca
//
LOCUS       DASUEG010000614         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1309808, whole genome shotgun sequence.
ACCESSION   DASUEG010000614 DASUEG010000000
VERSION     DASUEG010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309808"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1121
                     /locus_tag="VFX11_11575"
     CDS             150..1121
                     /locus_tag="VFX11_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HEX4939307.1"
                     /translation="MKQILTLDPATYRRHAIHGPDRVWAETNCYVDLLIELIHANGFE
                     PVAALPFTLGIGFEGDQWTFFKFSHHDLQTLFGIEIQELAIWRPVIDHIEEQVGAGKP
                     VLVELDSWYLPDTAGTAYQLDHVKTTVAVNLIDRANRRLGYFHNQGYFELQGDDFTQI
                     FQLDGLVHPRMLPPYVEFARFHDGGLHGQALLEASLAVLKHQLPLIPRSNPFTAFKAR
                     FAQDIDWLMQSDISLFHKYSFATLRQYGACFELAQTYTQWLQDQGVDGVAPATAAFAE
                     ISQGAKAFQFQLARAMARKKPLDLTPIDNMGAAWEKGMAALNLRFNR"
     gene            1124..>3520
                     /locus_tag="VFX11_11580"
     CDS             1124..>3520
                     /locus_tag="VFX11_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX4939308.1"
                     /translation="MTNPICSRAISSHHVDDVRTGWQLAATAADSISFEQLNAAGLQW
                     RDAIVPGTLASSLHPDLNEPGNYDCRDWWYRVTFAAPAGQGSQGVRHRLRFEGLATLA
                     DVWLNGHPLLSTSNMFCTEVVDVTGQLQESNTLVIRFRALETALELKRPRPRWKTALV
                     QHQNLRWFRTTLLGRIPAWTPAIEPVGPWRPVQLETVATVDVTALDLQTRLDGTTGVV
                     SVQAHIQSLQETPTTATLVVGEHRHALGMTRNGQAHDISGSCEIPHAPLWWPNTHGGQ
                     PLLPCRLELSIGDNVVTIDCGRIGFKSVQVNRSGGAVEFIINGVPVFCRGACWTVNDI
                     LSLAGSHESLRQKLQLARDANINMLRIGGTMIYEQDAFFRLCDELGILVWQDFMFANM
                     DYPVADPDFHASIVRETRQQLDLLQKHVCVAAYCAGSEIEQQAAMMGLPASEWTNPFF
                     AEELPQLCAQLHAGIPWFRNSPSEGALPFHVGTGISHYYGVGAYRRPLSDAKHAGVKF
                     ASECLGIANVPEPDTMALLLEGKLLAPHHPRWKARVARDAGSGYDFEDIRDFYLREIF
                     QLDPIQLRSENPERYYAVSRAVSGEVMLRTFAEWRRASNPCAGGLIWFYQDLWPGAGW
                     GIVDSENRPKAAWHYVRRAWAPRAILITDDGLDGIKLQLCNDSADAFEADIELGLLQH
                     GKVKTLERRLNRTLAPRSVAEVSVDETVGHFTDCNYAYRFGPAGHDTIYCRLIASGSG
                     QVVAEDVYMPLRKIAAMVEPHQIHTTVTALAEGGVELTLSADALLQDVTFTGTDYMPD
                     V"
BASE COUNT          676 a         1166 c         1041 g          637 t
ORIGIN      
        1 gtgttcaaga tcaacagcgc cgacggtgcc gtcgcgcaca ccgcctgcat gggctttggc
       61 ctggagcgga tctgtctggc gctgttcaag catcatggtc tgaacatcag ccagtggccg
      121 gaatcggtgc gcaaactgct ggacctgtca tgaaacagat actgacactg gacccggcga
      181 cctatcgacg ccatgccatt catggtccgg accgggtgtg ggcggaaacc aattgctacg
      241 tggatctgct gatcgagctg atccacgcca atggtttcga gcccgttgcc gcactgccgt
      301 tcaccctggg catcggtttc gaaggcgatc agtggacctt cttcaagttt tcccaccatg
      361 acctgcagac actgttcggc atcgagatcc aggagctcgc catctggcgc ccggtcatcg
      421 accatatcga ggaacaggtc ggcgcgggca aaccggtgct ggtggaactg gattcctggt
      481 acctgccgga taccgccggc accgcgtacc agctcgatca tgtgaaaacc acggtcgccg
      541 tgaacctgat cgaccgcgcc aaccggcgcc tgggttattt ccacaaccag ggctatttcg
      601 aactgcaggg cgatgacttc acccagattt tccagctgga cggcctggtg cacccgcgca
      661 tgctgccgcc ctacgtggaa tttgcgcgtt tccacgacgg tggcctgcac ggccaggcgc
      721 tgctggaggc gtcgctggcg gtgctgaagc atcagctgcc gctgattccg cgcagcaatc
      781 cgttcaccgc cttcaaggcg cgctttgccc aggacatcga ctggctgatg cagtcggaca
      841 tcagcctgtt ccacaaatat tcgttcgcca cgctgcgcca gtacggcgcc tgcttcgaac
      901 tggcccagac ctacacccag tggctgcagg atcagggcgt cgatggcgtc gcgccggcca
      961 cagcggcctt tgccgagatc tcgcagggcg ccaaggcgtt ccagttccag ctggcgcgcg
     1021 cgatggcccg caaaaaaccg ctggacctga cccccatcga caacatgggc gccgcctggg
     1081 aaaaaggaat ggcagcgctg aacctgcgct tcaaccgctg acgatgacga acccgatctg
     1141 cagccgagcc atcagctcac accatgtgga cgacgtgcgg acgggctggc aactcgccgc
     1201 caccgccgca gacagcatca gcttcgaaca actcaatgca gccggcctgc agtggcggga
     1261 tgccatcgtc cccggcaccc tggccagcag tctccacccc gacctgaacg aacccggcaa
     1321 ctacgattgc cgcgactggt ggtaccgcgt gaccttcgcg gcacccgcag gccagggcag
     1381 ccagggcgtg cgtcaccgcc tgcgctttga aggcctggcc accctggccg atgtctggct
     1441 caacggccat ccgctgctct ccaccagcaa catgttctgc accgaggtgg tggatgtcac
     1501 cgggcagctg caggaatcca acacgctggt catccgcttc cgcgcgctgg aaacggcact
     1561 cgaactcaag cggccacggc cgcgctggaa aaccgcactg gtgcagcacc agaacctgcg
     1621 ctggttccgc accacgctgc tgggccgcat tcccgcctgg acacccgcca tcgagcccgt
     1681 cggtccctgg cgcccggtgc aactggaaac cgtggccaca gtcgacgtga ctgcgctgga
     1741 cctgcagaca cggctggacg gaaccaccgg tgttgtcagc gtgcaggcac acatccagtc
     1801 actgcaggaa acacccacca ccgccacgct ggtggtgggt gagcaccgtc atgcactggg
     1861 tatgacccgc aacggccagg cgcacgacat cagcggcagc tgcgaaattc cgcacgcgcc
     1921 actctggtgg cccaacaccc atggcgggca accgttgctg ccgtgccggc ttgaactcag
     1981 catcggcgac aacgtcgtca ccatcgattg cggccgcatc ggattcaaaa gcgtgcaggt
     2041 caaccgcagc ggcggcgccg tggaattcat catcaatggc gtgcccgtgt tctgccgtgg
     2101 cgcctgctgg accgtcaacg acatcctgtc gctggccggc agccatgaat ccctgcgtca
     2161 gaaattacaa ttggcccgcg atgcgaacat caacatgctg cgcatcggcg gcaccatgat
     2221 ctatgagcag gatgcgtttt tccggctctg cgacgaactc ggcattctgg tatggcagga
     2281 tttcatgttc gccaacatgg actacccggt ggccgatccg gattttcacg ccagcattgt
     2341 gcgcgaaacc cgccagcaac ttgacctgct gcagaagcat gtctgcgtcg ccgcctattg
     2401 tgccggcagc gaaatcgaac agcaggcggc catgatggga ctgccggcct cggaatggac
     2461 caacccgttt tttgccgaag aactgccaca actgtgtgcg caactgcacg ccggcattcc
     2521 ctggttccgc aattcaccgt ccgaaggtgc gttgccattc cacgttggca ccggcatttc
     2581 ccattattac ggcgtcggcg cctatcgtcg cccgctcagc gatgccaaac atgccggcgt
     2641 caaatttgcc tccgaatgcc tgggcattgc caacgtgccc gaaccggaca ccatggccct
     2701 gctgctggag ggtaaactgc tggcaccgca ccatccgcgc tggaaggctc gggtcgcccg
     2761 cgacgccggc tccggctacg acttcgaaga catccgcgat ttctatctgc gcgaaatctt
     2821 tcagctcgat cccatccagt tgcgcagtga aaatccggag cgctattacg ccgtgtcgcg
     2881 cgcggtttcc ggcgaggtca tgctgcgcac ctttgcggaa tggcggcggg cgagcaaccc
     2941 ctgcgccggc ggtctgatct ggttctacca ggatctgtgg ccaggtgcgg gctggggcat
     3001 cgtcgacagc gaaaaccgcc cgaaggccgc gtggcattat gtgcgccggg cctgggcacc
     3061 gcgcgccatc ctgatcaccg atgatggtct ggacggcatc aaactgcagc tgtgcaacga
     3121 ttccgccgac gcgttcgaag ccgacatcga gctgggcctg ctgcagcacg gcaaggtgaa
     3181 aaccctggag cgacggctga accgcaccct cgcaccgcgg tcagtggcag aagtatcggt
     3241 ggatgaaacc gtcggtcact tcaccgattg caactacgcc tatcgcttcg gcccggccgg
     3301 gcacgacacg atttactgcc ggttgatcgc atccggttca ggtcaggttg ttgccgagga
     3361 tgtctatatg ccattgcgga aaattgcggc aatggtggaa ccgcaccaga ttcacacaac
     3421 ggtcactgca ctggcggagg gtggcgtgga actgaccctg tccgccgacg cactgctgca
     3481 ggacgtgacc ttcaccggca ccgattacat gcccgacgtc
//
LOCUS       DASUEG010000615         6984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2079698, whole genome shotgun sequence.
ACCESSION   DASUEG010000615 DASUEG010000000
VERSION     DASUEG010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079698"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VFX11_11585"
     CDS             complement(<1..1058)
                     /locus_tag="VFX11_11585"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX4939309.1"
                     /translation="MSSSTGKLESSKSNNLIPFYIIGLIVTVGLMLLNFYFVANHTEQ
                     SAKYVTKATELRVLSQRIAKNALEAAAGNADAFAFLDQARVEFGLVWDSIKNGAPDEG
                     MSPDSVFVTSAPEEMASVEKIWKQVSQSSDVILEGRQTVINLHDVAKALNNSIPVLQE
                     EYDVVVQTLVTTAAPGDQVALAQRQSLLAERIVRSVNKVLEGGNDAVMAADSFGQDTQ
                     RFGRVLTGMLEGDRSLGVTRVKETAAIESLNEISEQFAEVNESVNEILESAPELFQVR
                     GAANSIFTDSQTLLTETGALVKSFEGTPITGIWSTLFGVIFGVLALLFLLAIGFSFIG
                     QTRSRLAEEAQRREEEEK"
     gene            complement(1272..1811)
                     /locus_tag="VFX11_11590"
     CDS             complement(1272..1811)
                     /locus_tag="VFX11_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4939310.1"
                     /translation="MSEVSRAYLALADIAQLSRQHAQGLPAQEEARAIWSGVGFTLND
                     RRYVVSMEEVSEILSVPRYTQVPGVKSWVKGIANVRGRLMPVMDLLGFLNRPSALQLK
                     RRRLLALERGELYSGLVVDEVLGMQHIPQDTFTTTVPGEYAETRPYLKGGFATEKGFW
                     AVFSLYELARDPRFLNVAS"
     gene            complement(1820..2188)
                     /gene="pilH"
                     /locus_tag="VFX11_11595"
     CDS             complement(1820..2188)
                     /gene="pilH"
                     /locus_tag="VFX11_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilH"
                     /protein_id="HEX4939311.1"
                     /translation="MPRVLIVDDSPTETYKFKEILEKHGFQIITADNGADGVAVARQE
                     KPDVVLMDVVMPGLNGFQATRQLSKGDETKHIPVIIVTTKDQETDKVWGRRQGATDYL
                     TKPVDEAVLIDTIKRAMSGK"
     gene            complement(2256..2642)
                     /gene="pilG"
                     /locus_tag="VFX11_11600"
     CDS             complement(2256..2642)
                     /gene="pilG"
                     /locus_tag="VFX11_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEX4939312.1"
                     /translation="MEENFENLKVMVIDDSKTIRRTAETLLKKAGCTVITATDGFDAL
                     SKIADTQPNIIFVDIMMPRLDGYQTCALIKNNSAFKSTPVIMLSSKDGLFDKAKGRIV
                     GSDQYLTKPFSKEELLGAIREHVTAA"
     gene            2929..3882
                     /gene="gshB"
                     /locus_tag="VFX11_11605"
     CDS             2929..3882
                     /gene="gshB"
                     /locus_tag="VFX11_11605"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX4939313.1"
                     /translation="MTIKVGVVMDPIQSITYKKDSTLAMLWEADARGWSIFYMRQQDL
                     TIDNGRAMAHMTRLHPRHDPQHWYEFGEDTYAPLGNLDVILMRKDPPFDMPFIYTTYI
                     LDKAEADGVLVVNHPQSLRDCNEKVFATWFPHCCAPTLVTSQKQRLRAFAEEHGDVIL
                     KPLDGMGGASIFRTGAKDGNLNVIIETLTLHGRLPAMAQKYIPEISKGDKRILVVNGE
                     PVPFCLARIPMVGEVRGNLAAGGSGVVQPLSDRDRWICSQVRDTLIEKGLLFVGLDVI
                     GDYLTEINVTSPTCIREIDAGAGTNIAGLLLDAIETRLTRK"
     gene            3893..4780
                     /locus_tag="VFX11_11610"
     CDS             3893..4780
                     /locus_tag="VFX11_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003162015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4939314.1"
                     /translation="MAAVAERPTISASDRLTFTLFIAAIFHAMVILGVGFSFTEPQPA
                     ASSLEIVLAQSRSDTAPEQADFLAQANQQGSGDLDRSALPTTNELAQFEDSKIREIQP
                     LEQSATQKQVRPDDRPVITTQGRSQQKVAIANPSPDDQKVEISKEERLAMIQRSLEIA
                     SLEAQLEEQKQTHAKRPRKRQLTSVSTKESRDAYYLHAWSKKIETVGNLNYPAEARRN
                     KIYGSLRMMVAIRADGSVHEMRILKSSGYRVLDQAAMQIVRLAAPYSAFPPEIRQDTD
                     ILEIIRTWQFEKGGRLSSY"
     gene            4905..5477
                     /locus_tag="VFX11_11615"
     CDS             4905..5477
                     /locus_tag="VFX11_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX4939315.1"
                     /translation="MTALKSSSLKNHFLIAMPGMVDPNFAHSITYICEHNDDGAMGIV
                     VNRPMELTVADILSHLQIRDFDDQFGEVPVLCGGPVQMERGFVLHRTLPHKWDTTLCV
                     EGDICLTTSRDILVAIAHHEGPDESVIALGYAGWSAGQLDNEMAQNAWLSVKADPDII
                     FATPFEQRWQAAASLLGVDMALLSAQAGHA"
     gene            5583..6008
                     /gene="ruvX"
                     /locus_tag="VFX11_11620"
     CDS             5583..6008
                     /gene="ruvX"
                     /locus_tag="VFX11_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX4939316.1"
                     /translation="MAFDYGTQKIGVAVGQTQTLSATALPELRAKDGQPDWNAVAKLI
                     EEWQPDAFVVGVPVNMDGSEFDMTQRARKFGQRLHGRFGKPWFAMDERLTSFDARQQA
                     QGPLKGKGLVDSIAASLILQSWFTDEWPRIAKDADNENA"
     gene            5995..6501
                     /gene="pyrR"
                     /locus_tag="VFX11_11625"
     CDS             5995..6501
                     /gene="pyrR"
                     /locus_tag="VFX11_11625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX4939317.1"
                     /translation="MKMPEVEPLLASMTRALQEKIRTLPKAPAMVGIHTGGAWVAQAL
                     HTALGLQQPLGLLDISFYRDDFEDKGLHPRAKPSSLPFAIDDQHIILVDEVVMTGRTI
                     RAALNELFDYGRPASVLLVTLVDIPRRELPIQPDVTGIRMDLQPGQRVKLAGPTPLTL
                     NLIDRPSL"
     gene            6498..>6984
                     /gene="pyrB"
                     /locus_tag="VFX11_11630"
     CDS             6498..>6984
                     /gene="pyrB"
                     /locus_tag="VFX11_11630"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872945.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4939318.1"
                     /translation="MTAKTAPHQLQLNEQGQLRHFLTTEGLSRQLLTEILDTAESFVS
                     VGKQAVKKVPLLRGKTVVNLFFENSTRTRTTFELAAKRLSADVLNINISASSTSKGES
                     LMDMLWNLEYMDSDMFIVRHGDSGAPHFIAEFVCPHVAVINAGDGRHAHPTQAMLDMY
                     TI"
BASE COUNT         1460 a         2063 c         2005 g         1456 t
ORIGIN      
        1 tgtttctctt cctcttcgcg gcgttgtgct tcttccgcca gacgggaccg ggtttgtccg
       61 ataaacgaga aaccgatggc cagcaggaac agcagcgcca gtacaccgaa gatgacaccg
      121 aacagggtgg accagatgcc ggtgatcggc gtgccttcga aggatttcac cagagcaccg
      181 gtttcggtca gcagggtttg cgaatcggtg aagatgctgt tggccgcgcc gcgtacctgg
      241 aacagttccg gggcactttc cagaatctcg ttgacgcttt cattcacttc cgcgaactgc
      301 tcggaaatct cgttgaggga ttcaattgca gcggtttcct tcacgcgggt gacgcccagg
      361 gagcggtcgc cctccagcat gccggtgagt acgcggccga agcgctgggt gtcctggccg
      421 aagctgtccg ccgccatcac tgcgtcgttg ccgccttcca gtaccttgtt gaccgagcgc
      481 acgatccgct ctgccagcag tgactgccgc tgtgccaggg ccacctggtc gccgggggcg
      541 gcggtggtca ccagggtctg taccaccacg tcatactctt cctgcagcac cgggatcgag
      601 ttgttcagtg ctttcgccac gtcgtgcagg ttgatcacgg tctggcggcc ttccagaatc
      661 acgtcggaac tctgggatac ctgcttccag atcttctcca ccgacgccat ttcctcggga
      721 gcgctggtca cgaacaccga atccggtgac atgccttcat ccggcgcgcc gttcttgatc
      781 gaatcccaca ccagaccgaa ttccacccgg gcctggtcga ggaaggcgaa cgcatcggcg
      841 ttaccggcag cggcttccaa cgcgttcttc gcgatacgct gcgacagaac ccggagttcg
      901 gtagccttgg tcacatactt ggctgactgc tctgtgtggt tggccacaaa gtagaagttc
      961 agcagcatca gacctaccgt gacgatcagt cctatgatgt agaacggtat caggttattg
     1021 gacttgctgg attccagctt tccagtgctc gacgacatag gcggactcct ggccgtttgg
     1081 tgcacaacta ttgtgatggt ggattggtta aagacatctc taaaaacagg tccgactgcg
     1141 ccggttttta gagatgtctt caattaaatg taggcacgaa tgcccctgat gggtagaaat
     1201 acagatgatc catttccacc cggtttttcc ccgctccggt tacccgcagc ggcaaaaagc
     1261 gttcgcctcc ctcaggaggc cacgttgaga aagcgcggat cgcgcgccag ctcatacagg
     1321 ctgaatacag cccagaatcc tttttcagta gcaaaaccgc ccttcaggta aggccgggtt
     1381 tccgcatatt cgccgggcac cgtcgtggtg aaggtgtcct gtggaatgtg ctgcatgccc
     1441 agcacctcat ccaccaccag accgctgtag agttcgcccc gctccagcgc cagcagacgg
     1501 cggcgcttca gttgcaatgc ggaagggcga ttcaggaatc ccagcagatc catcaccggc
     1561 atcagccggc cgcgtacgtt ggcaatgcct ttcacccagc tcttgacgcc gggtacctgt
     1621 gtatagcggg gaaccgacag gatttcggac acttcttcca tcgacacgac atagcgacgg
     1681 tcattcagcg tgaagccgac gccgctccag atggctctgg cttcttcctg ggcaggcaga
     1741 ccctgggcgt gctgtcggct gagttgggca atgtcggcca gggcaagata ggcgcgtgaa
     1801 acctcggaca tagggtggct tattttccgc tcatggcgcg tttgatggtg tcgatcagaa
     1861 ccgcttcgtc caccggcttg gtcagataat cggttgcgcc ctggcggcgg ccccacacct
     1921 tgtcggtttc ctgatccttg gtggtgacga tgatcacggg aatatgcttg gtttcgtcgc
     1981 ccttgctgag ctggcgggtg gcctggaagc cgttcaggcc cggcatcacc acatccatca
     2041 gcaccacatc gggcttttcc tggcgggcca cggccacgcc atcggcgccg ttgtcggcgg
     2101 tgatgatctg aaagccgtgc ttttcgagga tttccttgaa tttgtacgtt tcagtgggcg
     2161 aatcatcaac aatcagaacg cgaggcataa cccctccgaa atcaaattaa agccggaaca
     2221 ttcagtgcag gtgcactcga acagtctatc gaccctcagg ccgcagtgac atgctctcga
     2281 atcgctccca gtagttcttc tttgctgaaa ggcttggtca gatattgatc ggagccgacg
     2341 atacggccct tggccttgtc gaacaggcca tccttgctgg acagcatgat taccggggtg
     2401 gacttgaaag cgctgttgtt cttgatcagc gcgcaggtct ggtagccgtc cagacgcggc
     2461 atcatgatgt cgacgaatat gatattgggc tgggtatcgg cgattttcga cagggcatca
     2521 aacccgtcgg tcgcggttat aacggtacac ccggcctttt tcagcagtgt ttcagcagtg
     2581 cgacgaatag ttttgctatc gtcgatcacc atgactttca ggttttcgaa attctcttcc
     2641 atcgacattg cctttgattc agagcgataa gtcagcaatc ttgtattagt tataatatcg
     2701 ttagtgggag ttcgcaaacc gaaagggtga ttaaatcttg tagttgcggg aagcagcatg
     2761 atttttatca cagtttttca gtgcaatcca taagcccctg ttgatcaagc gattttttaa
     2821 tctttaatta aagcgtgata tttataaccg gacagggcga tcccgtgtaa cctatatgct
     2881 gaaatccgtc ctgcccgcga cgttccgcac tgcaatcgag cacaccacat gaccatcaaa
     2941 gtcggggtgg taatggaccc catccaatcc attacgtaca aaaaagacag caccctggcc
     3001 atgctgtggg aggccgacgc gcggggctgg tcgatttttt acatgcggca acaggatctg
     3061 accattgata acggcagggc gatggcgcac atgacccgcc tgcatccgcg tcacgatccc
     3121 cagcactggt acgaattcgg cgaggatacc tacgcaccgc tgggtaatct cgacgtgatc
     3181 ctgatgcgca aggatccgcc cttcgacatg cccttcatct ataccaccta cattctggac
     3241 aaggctgagg ccgatggtgt cctggtggtg aatcaccccc agagcctgcg tgactgcaat
     3301 gaaaaggtgt ttgccacctg gtttccgcac tgttgcgccc caacgctggt caccagccag
     3361 aagcagcggc tgcgggcgtt tgccgaagag catggcgatg tgattctcaa gccgctggat
     3421 ggcatgggtg gcgcgtccat cttccgcacc ggtgcgaagg acggcaacct gaatgtcatc
     3481 atcgaaacgc tgacattgca tggccgcctg ccggcaatgg cacagaagta cattcccgag
     3541 atcagcaagg gcgacaagcg catcctggtg gtgaacgggg agcccgtgcc tttctgcctg
     3601 gcccgcattc ccatggtggg ggaagtccgc ggcaacctgg cggcaggggg ttccggtgtg
     3661 gtgcagccgc tgtcagatcg tgaccgctgg atttgcagtc aggtccggga tacattgatt
     3721 gaaaagggat tgctgttcgt gggcctcgat gtgatcgggg attatctcac cgaaatcaac
     3781 gtcaccagcc ctacctgcat tcgcgagatc gatgccggag ccggtaccaa tatcgccggg
     3841 ctgctgctgg atgccatcga aacccgtctg acacgaaaat aattacaacg acatggctgc
     3901 agttgccgaa cgccccacca tttccgccag tgaccgactg accttcacgc tgttcatcgc
     3961 ggcgatcttt cacgccatgg tgatcctggg ggttggcttt tcgttcaccg agccgcagcc
     4021 ggcggcgtcc tcgctggaaa tcgtgctggc acaaagccgc agcgataccg caccggagca
     4081 ggcggatttt ctggcccagg ccaaccagca gggtagcggc gatctggacc gtagcgcact
     4141 gcccaccacc aatgagctcg cgcaattcga agacagcaag atccgcgaga tccagccgct
     4201 ggaacaatcc gccacacaga aacaggtgcg acccgacgat cggcccgtta tcaccacgca
     4261 gggccgcagc cagcaaaaag tggcgattgc caacccgagc cctgatgacc agaaggtgga
     4321 gatcagcaag gaagagcggc tggccatgat ccagcgcagc ctggagatcg ccagcctgga
     4381 ggcacagctg gaagaacaga agcagactca cgccaagcgt ccgcgcaagc ggcagctgac
     4441 ttcggtcagc accaaggagt cccgcgatgc ctattacctg catgcctgga gcaagaagat
     4501 cgaaaccgtg ggtaacctga attacccggc agaggcgcgc cgcaacaaga tttacggcag
     4561 cctgcgcatg atggtggcga tccgtgccga cgggtcggtc cacgaaatgc gcattctgaa
     4621 atcctccggc taccgggtgc tggatcaggc ggcgatgcag attgtgcgtc tggcggcgcc
     4681 ctattccgct tttccgccag aaatccgtca ggataccgac atcctggaaa tcatccgcac
     4741 ctggcagttc gagaagggcg gccgcctgtc cagctactga ttcggctgca acgggaaaac
     4801 accttgtttt tgcggccatt ttggccattt ttaacgggta cgccccgccg ctcggcgcac
     4861 aaaattcact tgaaccaggg cgctgaagcc ccaatactgt agttatgact gcgctgaaaa
     4921 gttcgtccct caagaatcat ttcctgatcg ccatgcccgg catggtggat ccgaattttg
     4981 cccattccat tacctatatc tgcgaacaca atgacgacgg cgccatgggc atcgtggtga
     5041 accggccgat ggagttgacg gtggccgata tcctgagcca cctgcagatc cgcgatttcg
     5101 acgaccagtt tggcgaagtg ccggtgctgt gtggcgggcc tgtacaaatg gagcgcggct
     5161 tcgtgctgca ccgtacgctg ccgcacaaat gggacacaac cctgtgcgtg gaaggtgata
     5221 tctgcctgac cacctcgcgt gacattctgg tggcgatcgc ccatcacgaa ggccccgatg
     5281 aaagtgtcat cgcactgggt tatgccggct ggagcgcggg acagctggat aatgaaatgg
     5341 cgcagaacgc ctggctgagc gtgaaagccg atccggacat tattttcgcc acgccgtttg
     5401 aacagcgctg gcaggcggcg gcctcgctgc tgggcgtgga catggccttg ctgtctgcac
     5461 aggcgggaca tgcgtaagcg cgtggatgat gcgctgaacc gtaccgtcgt catttctttc
     5521 accccatttg gcaatccctc aatcccatgg ctgcatttcc ttcatcgcgc gctgtccgcc
     5581 tgatggcctt tgactacggc acccagaaaa tcggtgtcgc cgtgggtcag acgcaaactc
     5641 tctctgccac cgctttgccg gaactgcgcg cgaaggacgg ccagcccgac tggaacgccg
     5701 tggcaaaact catcgaggaa tggcagccgg atgcgttcgt ggtgggcgtg ccggtgaaca
     5761 tggacggcag cgaattcgac atgacccagc gcgcacgcaa attcggccag cgattgcatg
     5821 ggcgtttcgg caagccctgg tttgccatgg acgagcgcct cacgtcgttc gacgcgcggc
     5881 aacaggcaca gggaccactc aagggcaagg gtctggttga cagcattgcc gcaagcctta
     5941 ttctgcaatc ctggttcacc gacgagtggc cgcgaatcgc gaaggatgcc gacaatgaaa
     6001 atgcctgaag tggaaccact gctggccagc atgacccgcg ccctgcagga aaaaatccgc
     6061 acactgccca aagcacccgc catggtgggc attcataccg gcggcgcctg ggtggcgcag
     6121 gcgctgcata ccgcgctggg attgcagcaa ccgctgggcc tgctcgacat ttccttttat
     6181 cgcgatgatt tcgaggacaa gggcctgcat ccccgggcga agccgtccag cctgccgttc
     6241 gccatcgatg accagcacat cattctggtg gatgaggtgg tgatgaccgg ccgcaccatc
     6301 cgggcggccc tcaacgagct gttcgattac ggccgtccgg cgtcggtgct gctggtgacg
     6361 ctggtggaca taccgcgccg cgaactgccc atccagcccg atgtaacggg aattcgcatg
     6421 gatttgcagc ccggtcagcg ggtaaaattg gccggcccca cgcccctcac tctcaatttg
     6481 atcgaccgcc cgagtctatg acagccaaaa cagcgccgca ccagctgcag ttgaacgaac
     6541 agggtcaact gcgtcatttc ctcacgaccg aaggattgag ccggcaactg ctcaccgaaa
     6601 ttctcgacac ggcggaatcc ttcgtcagcg tcggcaagca ggcagtcaag aaagtgccgc
     6661 tgctgcgcgg caagacggtg gtgaacctgt tcttcgaaaa cagcacccgc acccgtacca
     6721 cctttgagct ggcggcaaaa cggctgtcgg cggacgtgct caatatcaac atcagcgcgt
     6781 cgtccacctc caagggtgaa tccctgatgg acatgctgtg gaacctggaa tacatggaca
     6841 gcgacatgtt catcgtgcgc cacggtgaca gcggcgcgcc gcatttcatc gccgaattcg
     6901 tctgcccgca cgtggcggtg atcaatgccg gtgacggccg tcatgcccat cccacgcagg
     6961 ccatgctcga catgtacacc atcc
//
LOCUS       DASUEG010000616         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1284611, whole genome shotgun sequence.
ACCESSION   DASUEG010000616 DASUEG010000000
VERSION     DASUEG010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284611"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFX11_11635"
     CDS             <1..478
                     /locus_tag="VFX11_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939319.1"
                     /translation="KRPLPRLVMLDSGFGVGLHPITFDADFQVLMFAVELGLSIQDFA
                     EFNQQPDLLTKLKWLKQYLATLHIEVTEELLLEWSVAYRKRLKSLETYGKGDELENAE
                     ITLIKAALHSHGRDDLGWGDTNKNIKWQTVQADHQSIVKHTEVAEWLRQFCQRQAG"
     gene            524..>2414
                     /locus_tag="VFX11_11640"
     CDS             524..>2414
                     /locus_tag="VFX11_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939320.1"
                     /translation="MSRIGWSGWGKGLCRLMMGSALLAGSVAASAAGFSAASSCNPVA
                     VNACGLPFPSDLFRNLTGTYNYSDSILDRNVNGATHQIFPVKSQFPATFQPSKIFNNS
                     NGFSALGPVLFELQNWPVHEIPADGAGHVVVFDMETDEQIPMVVSLSHVANPQRDFRE
                     KRPVIIAWPRSRFEFGRKYAAVLLKTPFDAAVGNTTTFVPSEGMAKVLTNRGGYFVQP
                     AYKGALDVVDRNGIARSDILSLTWFTVRTEADVTTPMKTMVQTALNYPSIVTRLEKRD
                     SLGDPEHGLVTLKGELSLVNFRSADGGVYPPYQPILDPALGRTDFVLTLPKWNQDTAI
                     PISIWGHGLGNFKELTKSGYVMGDRLGMATFAIDHPNHGSRIKVTGNKEPSIVVAVQS
                     PMTIMHLLGMFVQATVDHSVVINHAHQALPQALSQWRSSEYPNVPRLDGSRVMFDGMS
                     LGAMLGTAIGAVAPDLAGAYLVNGAGSLMHIFSESTFWDDMTSHVVPQNANGAELTFV
                     LAMMQHYVDIADGNNFVHYFRNPPAGQDVRPLGMHYSLGDGSMPNNATRASAEIADLP
                     LLKEVIEPEPFLRDGTLGRDGFEEGYGLVQSGYGLELADQIKDQIKAFDPTLGAGLDT
                     AGLLKK"
BASE COUNT          501 a          719 c          708 g          486 t
ORIGIN      
        1 gaaacgtccg ctgccccggc tggtgatgtt ggacagcggc ttcggcgtcg gcctgcaccc
       61 gatcacgttc gatgccgatt tccaggtgct gatgtttgcg gtggaattgg gcctttcaat
      121 tcaggatttt gccgaattca accagcaacc ggacctgctc acaaaactga aatggctgaa
      181 acaatatctc gctactttgc acattgaagt tacagaggaa ttgttgctag agtggagtgt
      241 cgcctatcgc aagcgtttaa aaagcctgga aacctacgga aagggagacg agcttgaaaa
      301 cgccgaaatc accctcatca aggccgcgtt gcattcgcac gggcgcgacg atctgggctg
      361 gggcgacact aataaaaaca taaaatggca aaccgttcag gcggaccacc agtccatcgt
      421 caagcatacc gaagttgccg aatggttgcg tcagttctgt cagcggcaag ccggttgacc
      481 gccgcgcaga gagtgcagag agagacgacc aatgggaaat actatgtcac gcatcggatg
      541 gtctggctgg ggcaagggat tgtgccggtt gatgatgggc tcggctttgc tggcgggatc
      601 ggtggccgcg tccgccgcag gtttctcggc ggccagctcc tgcaacccgg tggcggtgaa
      661 tgcctgtggc ctgccgttcc ccagcgatct tttccgcaat ctgaccggta cctacaatta
      721 cagcgattcc atcctggatc gtaacgtcaa cggcgccact caccagattt tcccggtaaa
      781 aagccagttc cctgccacgt tccagcccag caagattttc aacaacagca acggcttttc
      841 cgcgctgggg cctgtactgt tcgaattgca gaactggcca gtgcacgaga ttcctgcaga
      901 cggtgccggc catgtggtgg tgttcgacat ggaaaccgac gagcagattc ccatggtggt
      961 gtctctcagt catgtcgcca atccgcagcg tgacttccgc gaaaagcgcc cggtgatcat
     1021 tgcctggccc cggtcccgct ttgaatttgg caggaagtac gcggcggtac tgctgaaaac
     1081 cccgttcgat gcggccgtag gcaataccac caccttcgta cccagcgaag gcatggcaaa
     1141 ggtgctcacc aacaggggtg gctattttgt ccagcctgcg tacaagggcg ccctggacgt
     1201 ggtggaccgc aatggcatcg cccgcagtga cattctgtcc ctgacctggt ttaccgtgcg
     1261 caccgaggcc gatgtcacca cgcccatgaa aaccatggtg cagactgccc tgaactaccc
     1321 cagcatcgtc accaggctgg aaaagcgcga cagcctgggt gatcccgagc acgggctggt
     1381 gacgctgaaa ggcgagctgt ccctggtgaa cttccgttcc gcggatggtg gcgtttaccc
     1441 tccttaccag ccgattctgg acccggccct gggccgcacg gattttgtcc tgaccctgcc
     1501 caaatggaac caggacactg ccattcccat ttcgatctgg ggtcatggcc tgggtaactt
     1561 caaggaactc accaaatccg gctatgtcat gggtgatcgt cttggcatgg ccactttcgc
     1621 catcgaccac ccgaaccacg gcagccgcat caaggtcacc ggcaacaagg agccctcgat
     1681 cgtggtggcg gtgcaatcgc ccatgaccat catgcacctg ttgggcatgt tcgtgcaggc
     1741 cacggtggac catagcgtgg tgatcaatca tgcgcaccag gctctgccgc aggccctgtc
     1801 ccagtggcgc agcagcgaat accccaatgt gccacggctg gacggttccc gcgtgatgtt
     1861 tgacggcatg tcgctgggcg caatgctggg cacggccatt ggcgcggtgg cgcccgatct
     1921 ggcaggagcg tacctggtga acggtgccgg cagcctgatg cacatcttct cggaatccac
     1981 cttctgggat gacatgacca gccacgtggt gccgcagaac gccaacggcg ccgaactcac
     2041 cttcgtgctg gccatgatgc agcattacgt cgacatcgcc gatggcaaca acttcgtcca
     2101 ctacttccgc aatccgccgg ccggccagga tgtgcgtccc ctgggcatgc actattccct
     2161 gggcgatggc agcatgccca acaatgccac ccgtgccagc gccgagatcg ccgatctgcc
     2221 gctgctgaag gaagtcattg agccggagcc tttcctgcgg gatggcacgc tgggccgtga
     2281 cggtttcgaa gagggttacg gtctggtgca aagtggatac ggtctggaac tggcggatca
     2341 gatcaaggac cagatcaagg ctttcgatcc cacccttggg gctgggctcg acacggcagg
     2401 tctgctgaag aaac
//
LOCUS       DASUEG010000617         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1128016, whole genome shotgun sequence.
ACCESSION   DASUEG010000617 DASUEG010000000
VERSION     DASUEG010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128016"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1104
                     /locus_tag="VFX11_11645"
     CDS             148..1104
                     /locus_tag="VFX11_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939321.1"
                     /translation="MRKPVTDAELQQTQLFRDVQLDRIRPLLASCRKVFLQSGEVLLR
                     PEFSNNCMYLLLKGRVSVHLQNLDSDPLLTLTEGECVGEMSVFDGRNPSAYVKAADAV
                     EVLVIESEILWQLIDASHSVARNLLHILANRLRSGNANLSDSQSRQRETEQAANSDAL
                     TGLNNRRWLEEMLVRFKGKAINELQPLSVLMLDVDHFKRFNDTFGHKAGDLVLQMVAQ
                     NMRKRLRPSDMVARYGGEEFLILLPHTAASEAQVVAERLRKGVETCKVDAEQGQELPP
                     VTISVGVAPWRNGDSLELMVEAADKALYRAKANGRNRVELRD"
     gene            complement(1143..1937)
                     /locus_tag="VFX11_11650"
     CDS             complement(1143..1937)
                     /locus_tag="VFX11_11650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939322.1"
                     /translation="MALTTALVTGASSGIGYEISKVLAQEGHDLVLVARREERLQQLA
                     QYLEKDFNIEVTVLAADLAQISEIDRIFDTLMERNIPVDILVNNAGLGSLGPFTADDR
                     NTEVEQIQVNVIATTYMTKKFAVYMVKRGLGKILNIASLSGFTPWPYMAVYGATKAYI
                     IEFSEALRAELKGTGVSVTVSCPGPTSSEFHKVSGTDHLGLLRYIPVTGSAQMAKASI
                     RAMHKRKGVVVHGAMSLGYYWLARLLPRSFMLRLMKILLKPRWSGF"
     gene            2119..>2812
                     /locus_tag="VFX11_11655"
     CDS             2119..>2812
                     /locus_tag="VFX11_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939323.1"
                     /translation="MTTSDPPHIDPSQLRPEISMPLIRTVLGCVMRHGLDADIFLRDL
                     QLENAPLHDPSRRIDLAVYDQVQERAMQVLNDPALGLHMGEHTSPNTLGLLGFLLLSA
                     PTLGDAIDQWMHFHSLVSDAEPSRLLPQGDEVLFEYQFPRSTPDCNRIRAEFGLVQMA
                     KLAGLMLGGTLPVTEVRFEHPRPDYAEEYQRVFPGPVHFGCPHTAVLFPAAILRQPLP
                     SANAGVHELLKEE"
BASE COUNT          544 a          827 c          839 g          602 t
ORIGIN      
        1 tatcacaatc cctgtccact ccggcataaa tgcgaatatt ttccagccaa aaggttgcgt
       61 ttggcgccat gccggataac tttagtgtta ccagtgatcc atctttgtga cagacccatg
      121 gtttttcctc taccgcgaca cgatgacatg cgcaagccgg taactgacgc ggaactgcaa
      181 caaacccagc tgtttcgcga cgtgcagctg gaccgtatcc ggccgctgct ggcgtcgtgc
      241 cggaaagtct ttctgcaatc cggtgaagtg ctgctgcggc ctgagttcag caacaactgc
      301 atgtatctgc tgctgaaagg ccgcgtttcg gtgcatctgc agaatctcga cagcgatccc
      361 ctgctgacgc tcacagaagg cgagtgcgtg ggggaaatgt cggtgtttga cggtcgcaat
      421 ccatccgctt acgtcaaggc ggcggatgcg gtggaagtgc tggtcatcga aagcgagatt
      481 ctctggcagc tgattgatgc ctcccacagc gtggcacgca atctgctgca catcctggcc
      541 aaccgtctgc gttcgggcaa tgccaacctg tctgacagcc agtcccgcca gcgcgaaacc
      601 gagcaggccg ccaacagcga cgccctcacc ggactcaaca accggcgctg gctggaggaa
      661 atgctggtac ggttcaaggg caaggcgatc aacgaattgc agccgctgtc ggtgctgatg
      721 ctcgatgtgg atcatttcaa gcgcttcaac gacaccttcg gccacaaggc cggcgatctg
      781 gtgttgcaga tggtggcgca gaacatgcgc aagcgcctgc gtcccagcga catggtggcc
      841 cgctacggcg gcgaggaatt cctgattctg ctgccccata ctgccgcgag cgaagcgcag
      901 gtggtggcgg agcgtctgcg caagggcgtg gaaacgtgca aagtggatgc ggagcagggc
      961 caggaattgc caccggtgac gatatcggtt ggcgtggcgc cctggcgcaa tggtgattcc
     1021 ctggaactca tggtggaagc ggccgacaag gcgctctacc gtgccaaggc caacggccgc
     1081 aaccgggtgg aattgcggga ctaagttctg ttaaaactag gttccgttaa gactaggttc
     1141 cgttaaaacc cgctccaccg cggtttcagc aggatcttca tcagccgcag catgaaactg
     1201 cgcggcagca gacgtgccag ccagtagtag cccagactca ttgcaccgtg taccaccaca
     1261 cccttgcgct tgtgcatggc cctgatggac gctttggcca tctgtgccga gccggtgacg
     1321 ggaatgtagc gcaacaggcc gaggtggtcg gtccctgaca ccttgtggaa ttccgatgag
     1381 gtgggcccgg ggcagctgac agtgaccgat accccggtgc ctttcagttc tgcccgcaag
     1441 gcttcgctga attcgatgat gtacgccttg gtggcaccat agacggccat gtatggccag
     1501 ggcgtgaaac ctgacagcga cgcgatattc agaatcttgc ccagcccccg cttcaccatg
     1561 tataccgcga atttcttggt catgtaggtg gtggcgatca cgttcacctg gatctgttcc
     1621 acttcggtgt tgcgatcgtc ggcggtgaag gggcccagtg agcccaggcc cgcgttgttt
     1681 accaggatgt ccaccggaat gttgcgctcc atcagcgtgt cgaagatgcg gtcgatctcg
     1741 ctgatctgcg cgagatctgc tgccagcacg gtcacctcga tgttgaaatc cttttccaga
     1801 tattgcgcca gttgctgcag tctttcttcc cgccgggcca ccagtaccag atcgtggcct
     1861 tcctgcgcca gaaccttgct gatttcatag ccgatgccac tggaagcgcc ggttactagt
     1921 gcggtggtca gagccatcgg gtcgatcctg tcgccaaaaa agggaagcta cctatgttaa
     1981 tggcttttgt tgcaatagca atcggttgcc catgcgtcga cgcagtggcc gaggttccgg
     2041 tccgccgtgg tttcggtgta ggcttggcgc tgaaatccat atcccttgca ccggattccc
     2101 gcaccctggt tttgatgcat gacgacctct gatccaccgc acattgatcc atcgcagttg
     2161 cgccccgaaa tttccatgcc cttgatccgc accgtgctcg gttgcgtgat gcggcacggc
     2221 ctggacgcgg acatattttt acgggatttg cagctggaaa atgcgcctct gcacgacccg
     2281 tcccggcgca ttgacctggc ggtgtacgat caggtgcagg agcgcgccat gcaggtgctg
     2341 aacgatcccg cgctgggcct gcacatgggc gagcacacct cacccaatac gctcgggctg
     2401 ctgggttttc tgttgttgag cgcacccact ttgggggatg ccatcgacca gtggatgcat
     2461 ttccacagcc tggtgagcga tgccgaaccg tcccgtctgt tgccgcaggg cgacgaggtg
     2521 ctcttcgaat accagtttcc ccgctccacg ccggactgca accgcatccg cgccgaattc
     2581 ggtctggtgc agatggccaa actggcgggg ctgatgttgg gaggaaccct gccggtgacg
     2641 gaagtgcgct tcgaacatcc acggccggac tatgccgaag aataccagcg agtgtttccc
     2701 gggcccgttc attttggttg cccgcacacc gcggtgctgt ttcctgccgc gatcctgcgt
     2761 cagccgctgc catcagccaa tgcgggtgtg catgaactgc tgaaggaaga ag
//
LOCUS       DASUEG010000618         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_979446, whole genome shotgun sequence.
ACCESSION   DASUEG010000618 DASUEG010000000
VERSION     DASUEG010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979446"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /gene="nuoF"
                     /locus_tag="VFX11_11660"
     CDS             <1..1273
                     /gene="nuoF"
                     /locus_tag="VFX11_11660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX4939324.1"
                     /translation="ARPLTQHIGAGKVPPGLAEYQRAGGYEGFRKIVQMKPAEVVELV
                     KASNLRGRGGAGFPTGMKWSFVPAGSESQQQKYIIANADEMEPGTFKDRWLLEGDPHQ
                     FIEGMMIAAYAIQASVGFIFIRSEYTLAARRLQAAIEENRQARLLGDNILGTGFHFEL
                     FLHSSAGRYICGEETALINSLEGRRAVPRAKPPFPQVSGLWGKPTVVNNVETLCNLPH
                     IAKNGAEWFHELGREKDAGTKIFGASGRVKRPGAWELPMGTPVREILEQYAGGMQDGY
                     KFRGFLPGGGSTDFLVEQHLDLPMDYDVIGKAGSRMGTGQLIVLDDKTCPVAMVLNLI
                     RFFAQESCGWCTPCRDGLPQVEYLLLQVENGQGKPEDLKKLETFTQFLAPGNTYCALA
                     PGAVEPLQSALKYFREDFEQHIQQKTCPYRH"
     gene            1322..>2563
                     /gene="nuoG"
                     /locus_tag="VFX11_11665"
     CDS             1322..>2563
                     /gene="nuoG"
                     /locus_tag="VFX11_11665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX4939325.1"
                     /translation="MTTIFIDDEKYTVEGDKNLLQAVLELGLDLPYFCWHPAMGSVGS
                     CRQCAVKQFQSAEDQRGRIVMACMTPVSEGLRVSLKDTTAQTFRDQCIEALMTNHPHD
                     CPVCEEGGECHLQDMTNLSQHKVRVYRGKKRTFRNQKLGPFINHEMNRCITCYRCVRF
                     YRDYAGGRDLHAMASRNHVYFGRHEPGTLENVFSGNLVEVCPTGVFTDKTFSQHFARK
                     WDLQSSASICVHCAHGCNISPGERGGMLRRIVNRYNSSVNGYFLCDRGRFGYEFVNSD
                     KRVTAPMIDQAPVVTLKDEPSFASRLATDLQQANLIGIGSPRASVEANFALMEWVGKK
                     NFYAGVSQQELQALQCVLNILGTNPATTPTLKEIEQADAVLILGEDVLNTAPRLALAV
                     RQANRNKAKAMAAMAHIRSWDA"
BASE COUNT          583 a          642 c          771 g          567 t
ORIGIN      
        1 ggcaaggcca ctgacacaac acattggcgc aggcaaagta ccgccgggat tggcggaata
       61 tcagcgcgca ggtggttatg aaggattcag gaaaatcgtg cagatgaaac cggctgaggt
      121 ggttgagctg gtcaaggcat ccaatttgcg cgggcgggga ggggcgggat ttcccaccgg
      181 catgaaatgg agtttcgtgc ctgccggctc cgagagtcag cagcaaaaat atatcatcgc
      241 caatgccgac gagatggagc ccggcacctt caaggaccgg tggttactgg agggtgatcc
      301 gcatcaattc atcgaaggca tgatgattgc cgcgtatgcg atacaggcat cggtcggatt
      361 catctttatt cgctctgagt acacgcttgc tgccagacga ttgcaggcgg ccatcgagga
      421 aaaccggcag gcacgattgc tgggcgacaa cattctggga acaggatttc attttgagct
      481 gttcctgcat tccagcgccg gtcgctatat ttgcggcgaa gaaaccgcgc tgatcaattc
      541 gctggaaggg cgccgtgcgg tgccacgtgc gaagccgcca tttccgcaag tgtctggcct
      601 gtggggaaaa cccacggtgg tcaataacgt cgaaaccctg tgcaatctgc cgcatatcgc
      661 taaaaatggt gccgaatggt ttcacgaact tggccgtgaa aaggatgcag gaaccaagat
      721 ttttggtgcc agtggccgtg tgaagcgacc gggtgcgtgg gaactgccga tgggaacccc
      781 tgtgcgggaa atactcgaac aatacgcagg cggcatgcag gacggctaca aattcagggg
      841 ttttctgccc ggcggcggtt cgactgattt tctggttgag cagcatctgg acttaccgat
      901 ggattacgac gttatcggca aggccggtag ccgcatgggc accgggcaac tgatcgtgct
      961 ggatgacaag acctgcccgg tggccatggt gttgaatctg atccgtttct ttgcccagga
     1021 gtcctgcggc tggtgcacgc cgtgtcggga cggattgcca caagtcgaat acctgctgtt
     1081 gcaggtggaa aacgggcagg ggaaacccga agacctgaaa aagctggaaa cattcacgca
     1141 gtttctggcg ccagggaaca cctattgcgc actggcgccg ggtgctgtcg aaccgttaca
     1201 aagtgcattg aagtattttc gtgaagattt tgagcagcac attcaacaaa agacgtgtcc
     1261 gtaccgtcac tgaatgactt gatccgctgg cagttcaatt tgccgtgata aggaattcca
     1321 aatgaccacc atttttatcg acgatgaaaa atatacagtc gagggcgata aaaacctgct
     1381 tcaggccgtt ttggaactgg gtttggattt gccgtatttt tgttggcatc ctgccatggg
     1441 atcagtgggt tcctgccggc aatgcgcggt caaacagttt cagtcggcgg aagatcagcg
     1501 tggtcgcatc gtcatggcct gcatgacacc ggtcagtgaa gggctacggg tctcactcaa
     1561 agacacgact gcacagactt ttcgcgatca gtgcattgaa gccctgatga cgaatcaccc
     1621 gcacgattgc ccggtgtgcg aggaaggcgg ggagtgccat ctgcaggaca tgaccaatct
     1681 gtcccagcac aaggtgcgcg tctatcgcgg caaaaaacgc acctttcgca accagaagct
     1741 gggcccattc atcaatcatg agatgaatcg ctgcattacc tgttaccgct gtgtgcgctt
     1801 ttatcgtgac tatgcgggcg ggcgcgatct gcatgccatg gcgtcgcgca atcatgtgta
     1861 cttcggccgc catgaacccg gcaccctgga aaatgtgttc agcggcaatc ttgttgaagt
     1921 gtgccctacc ggtgttttta ccgataaaac cttcagccag cattttgcgc gcaaatggga
     1981 tctgcagagt tcagcgtcga tctgtgtgca ttgcgcgcat ggttgcaata tcagcccggg
     2041 cgagcgcggc ggcatgctac ggcgtatcgt caatcgctat aacagttcgg tcaacggcta
     2101 ttttctgtgt gaccgggggc gtttcggcta tgaatttgtc aatagcgaca agcgtgtcac
     2161 tgcgccaatg attgatcagg cgcccgttgt gacgctgaaa gatgaacctt cgtttgcgtc
     2221 gcggctggca accgatctgc aacaggccaa tctgattggt attggctcgc cgcgggcgtc
     2281 ggtggaagcc aattttgcgt tgatggaatg ggttggcaag aagaattttt acgccggtgt
     2341 cagccagcag gagctgcagg ccctgcaatg tgtgctcaat attttgggta ccaatccggc
     2401 caccacaccc acgctcaagg aaattgaaca ggccgatgct gtattgattc tgggggagga
     2461 tgtgctcaac acggcgccac gactggcgtt ggcggtcagg caagccaacc ggaacaaggc
     2521 aaaggccatg gcggccatgg cgcatattcg cagctgggat gcg
//
LOCUS       DASUEG010000619         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_933517, whole genome shotgun sequence.
ACCESSION   DASUEG010000619 DASUEG010000000
VERSION     DASUEG010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933517"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2060
                     /gene="tssH"
                     /locus_tag="VFX11_11670"
     CDS             <1..2060
                     /gene="tssH"
                     /locus_tag="VFX11_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX4939326.1"
                     /translation="PVLGRDREIRQLIDILLRRRQNNPILTGEAGVGKTAVVEGFALR
                     IANDDVPEPLRGVAVHTLDLGLLQAGASVKGEFENRLKSVIEEVKGSAQPIIMFIDEA
                     HTLIGAGGKEGQGDAANLLKPALARGELRTIAATTWAEYKKYFERDPALTRRFQVVKV
                     EEPEEERAINMMRGIAQSLEQHHKIRILDEAIVASVKLSHRYIPGRQLPDKSVSLLDT
                     ACARVALSQTATPAAIEDCRRRIRQDSANIASLEQENASSGIYDERIATLHAAKIQAE
                     EELKALEAQWQQELELIAKLRDLRLQIEADFLQGKKGEERALDDEQVAQLKAKLKDVT
                     AQLEAIQGDNPLMQAAVDEQAVADVVANWTGIPVGKMVSDEIQSVLNLHDRLGERVIG
                     QPHALEAVAQAIRTSRAGLTDPRKPIGVFLFVGTSGVGKTETALALADILYGGEQNLT
                     VINMSEFKEEHKVSMLLGSPPGYVGYGEGGVLTEAARRKPYSIILLDEMEKAHPGVQD
                     IFYNLFDKGTIKDGEGRDIDFKNTVIIMTSNAGEDAIRAIFSQAEERPAPDVVLDNIR
                     PHLLRHFKPAFLGRTSLIAYNPLDDENLMKIAAINMRRIEKRVKGHYGASFSYDEDVL
                     LHIVARCQEAETGARNIENILNKSLLPELASECLNRMANNEPINHVHIGVNEEGNFTY
                     SIR"
     gene            2139..3647
                     /locus_tag="VFX11_11675"
     CDS             2139..3647
                     /locus_tag="VFX11_11675"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX4939327.1"
                     /translation="MSRATISTGSIPLRQGPSSTTADSQRCHVLILADFSGRAHRGLN
                     DATHLASRKIIEVDRDNLEEVFTQLNVTLQLPLADAPIRFREFDDLHPDFIHERVPLF
                     DQLRALKRRLNNPATFAAAAAEISGPGGAEKTAAATGGTTQSASDDLLDSLLQSPTAQ
                     AASGGDVQALIRQIAAPYVIPRPDPRLDELQNAVDESSAQLLRKILHSSAFQSLEASW
                     RGLQLLTRRLETDGNLKLFIADISLDEIVADAEQDADDANAENTPVSQLHKLLVEQRA
                     GTGATPFGLILGDYRFEDQPQQVSALALLGDIAQQLGSSFIAGGSERLAGCTALHVQP
                     DPDDWTLTPDTEFTDCWQTLREDEVSQHIALVAPRFLLRLPYGKRTSPISAFAFEELP
                     AQDAHDFYLWGNGGWLVTLLLADAWSRNGKPTSSPAADIGDLPLHVRQQAGESQVTPC
                     AEILMTDTTASALQQAGLTVIRSVQNQDVVRVTSHFSLSDTGTLLRGRWTLA"
     gene            3826..>4114
                     /locus_tag="VFX11_11680"
     CDS             3826..>4114
                     /locus_tag="VFX11_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939328.1"
                     /translation="MPSKKMKTQNGNSCAAHCTIVAVAELTNSNKNLDETYTESNLWP
                     DIQFKPEGGAPATQLLAAAKNSDPRRIVAEVKKRWSGVTAKLVCDEVQKNKA"
BASE COUNT          950 a         1321 c         1181 g          662 t
ORIGIN      
        1 atccggtcct cggacgcgat cgcgagatcc gccagctcat cgacatcctg ctgcggcggc
       61 ggcagaacaa cccgatcctg accggtgaag ccggcgtcgg caaaaccgcc gtggtggaag
      121 gtttcgccct gcgcatcgcc aatgacgacg tacccgaacc gctgcgcggc gtcgcagttc
      181 acacgctgga cctgggcctg ctgcaggccg gcgccagtgt gaaaggcgaa ttcgaaaacc
      241 gcctgaaatc cgtgatcgag gaagtgaaag gttcggcgca gcccatcatc atgttcatcg
      301 acgaggcgca cacactgatc ggcgccggcg gcaaggaagg ccagggcgac gccgccaacc
      361 tgctgaaacc ggcactggcg cggggtgaac tgcgcaccat cgccgcgacc acctgggctg
      421 aatacaaaaa atatttcgag cgtgatcccg ccctcacccg ccgcttccag gtagtcaaag
      481 ttgaagagcc ggaagaggaa cgcgccatca acatgatgcg cggtattgcg caatcgctcg
      541 aacagcacca caagatccgc attctggacg aagccatcgt cgcgtcggtg aagctgtcgc
      601 accgctatat tcctggccgc cagctgcccg acaaatccgt cagcctgctc gacaccgcct
      661 gcgcccgcgt tgccctgagc cagaccgcaa cccccgccgc cattgaggac tgccgccgcc
      721 gcatccgcca ggacagcgcc aacatcgcct cgctggaaca ggaaaatgcc agcagcggga
      781 tttatgacga gcgcatcgca acactgcacg ctgcaaaaat ccaggcagaa gaagaactga
      841 aagccctgga agcccagtgg caacaggaac tggaattgat cgccaaactg cgcgacctgc
      901 gcctgcagat cgaagcggat ttcctgcagg gtaaaaaagg cgaggaacgc gcactggacg
      961 acgagcaggt cgcgcaactg aaagccaaat tgaaagacgt caccgcgcaa ctggaggcaa
     1021 ttcagggcga caatccgctg atgcaggccg ccgtggacga acaggcggtg gccgacgtgg
     1081 tggccaactg gaccggcatt cccgtgggga aaatggtcag cgacgaaatc cagtccgtgc
     1141 tcaatctgca cgatcgtctg ggcgagcgcg tgattggcca gccgcacgca ctggaagccg
     1201 tggcgcaggc aatccgcacg tcgcgcgcag gcctgaccga tccgcgcaaa cccatcggtg
     1261 tgttcctgtt tgtcggcacc agcggcgtcg gcaaaaccga aaccgcattg gcactggcgg
     1321 acattctgta cggcggcgag cagaacctca ccgtgatcaa catgtcggag ttcaaggaag
     1381 agcacaaggt gtccatgctg ctgggctcgc ctccgggcta cgtgggttac ggcgaaggcg
     1441 gcgtgctgac cgaagcggcg cggcgcaagc cctactccat catcctgctg gatgaaatgg
     1501 aaaaagccca tccgggcgta caggatattt tctacaacct tttcgacaag ggcaccatca
     1561 aggacggcga aggccgcgat atcgatttca agaacaccgt gatcatcatg acgtcgaacg
     1621 caggcgagga tgcgatccgc gccatcttca gtcaggccga ggaacgtccc gcgccggacg
     1681 tggtactcga caatatccgc ccgcacctgc tgcgtcattt caagccggcg ttcctgggtc
     1741 gcacctcgct gatcgcctat aacccgctcg acgacgagaa cctgatgaag atcgctgcca
     1801 tcaacatgcg gcgcatcgaa aagcgggtga aagggcatta cggcgccagc ttctcgtatg
     1861 acgaggacgt gctgctgcat atcgtggccc gttgccagga agcggaaacc ggcgcgcgca
     1921 acattgaaaa tattctgaat aaatccctgc tgccggaact ggccagcgaa tgtctgaacc
     1981 gcatggccaa caacgaaccc atcaaccacg tgcatatcgg cgtaaacgag gaaggcaatt
     2041 tcacctacag catccgctga ccttcttccc agagcgggta tcgttgccag cgcaacggcc
     2101 cgccctgctt tcaccccatt ctttctcagg agccttccat gagccgagcc acgatctcca
     2161 ccggatccat cccgctgcgg cagggcccgt catccaccac cgccgacagc caacggtgcc
     2221 atgtactgat actggcggat ttcagcggac gtgcccatcg cggcctgaat gacgcaacgc
     2281 atctggcgtc gcgcaagatc atcgaagtgg accgcgacaa cctggaagaa gtcttcacgc
     2341 aactcaatgt gacgctgcaa ctgccgctgg cagatgctcc gatccgcttt cgcgaattcg
     2401 acgacctgca cccggatttc atccacgagc gggtaccgct gttcgatcag ctgcgcgcgc
     2461 tgaaacggcg gctgaacaat cccgccacgt ttgcggcagc ggcggcggaa atcagtggcc
     2521 caggcggcgc ggaaaaaacg gcagcagcaa caggcggcac aacacaatca gccagcgacg
     2581 atctgctgga cagcctgctg caatccccca ctgcgcaagc cgccagcggt ggcgacgtgc
     2641 aggcgctgat ccgccagatc gccgcaccct acgtcattcc ccgccccgat ccgcgtctgg
     2701 atgaattgca gaacgcggtg gacgaatcca gcgcgcaact gctgcggaaa atcctgcaca
     2761 gcagcgcgtt tcaatctctg gaagccagct ggcgtggact gcaactgctg acccggcgtc
     2821 tggaaactga tggcaatctg aaactgttca tcgccgacat cagcctggac gaaatcgtcg
     2881 ccgacgcgga acaggacgct gatgatgcca atgcggagaa cacacccgtc agccagctgc
     2941 acaaactgct ggtggagcaa cgggccggca ccggcgccac gccgttcggt ctgatcctgg
     3001 gggattaccg ttttgaagac cagccgcagc aggtcagcgc gctagcactg ctgggcgata
     3061 tcgcgcagca actgggtagc agtttcattg ccggtggatc ggagcgactg gcgggctgca
     3121 cagccttgca tgtacaacct gatcccgacg actggacact gacacccgac accgaattca
     3181 ccgactgctg gcaaaccctg cgggaagatg aagtcagcca gcatattgcg ctggtggcac
     3241 cgcgctttct gctgcggctg ccctacggca agcgcaccag ccctatcagc gcctttgcgt
     3301 ttgaagagtt gccggcacag gacgcccacg atttctacct gtggggtaat ggcggctggc
     3361 tggtgacact gctgctggcg gatgcctggt cccgcaacgg caagcccacg tccagccccg
     3421 ccgccgacat cggtgatctg ccactgcatg tgcgccagca agcaggtgaa tcccaagtga
     3481 caccctgcgc cgaaatcctg atgaccgaca ccaccgcaag cgccctgcag caggcgggcc
     3541 tgactgtcat ccgctcggtg cagaaccagg atgtggtgcg ggtgacaagt cacttctcgc
     3601 tgagtgacac gggcacgtta ctgcgaggcc gctggaccct agcgtaaacc cggcacatca
     3661 accgccggat tgccctttgt gcattattgc catcctggca gctgaacaag taccggcgcc
     3721 catcgggcgc cggatgctgg cacgcccatt gcctcaatca gataccaatg gcgcagcctg
     3781 actgtcgcct tgaccacgct cacatcggaa agaggacact accccatgcc atcaaaaaaa
     3841 atgaaaaccc agaacggaaa ttcctgtgcc gcccattgca cgattgtcgc cgtggccgaa
     3901 ctcaccaatt ccaacaaaaa tctcgacgaa acctacaccg aaagcaatct gtggcccgac
     3961 atccagttca agcccgaagg cggcgcaccc gccacacaat tgctggcagc ggccaaaaac
     4021 agcgatccgc gccgtatcgt agcagaagtg aaaaaacgct ggagcggtgt caccgccaaa
     4081 ctggtgtgcg acgaagtgca gaaaaacaag gcca
//
LOCUS       DASUEG010000620         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1066723, whole genome shotgun sequence.
ACCESSION   DASUEG010000620 DASUEG010000000
VERSION     DASUEG010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066723"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="atpD"
                     /locus_tag="VFX11_11685"
     CDS             complement(<1..1104)
                     /gene="atpD"
                     /locus_tag="VFX11_11685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4939329.1"
                     /translation="MSSGRIVQIIGAVIDVEFPRDSVPNVYDALKVDTVTTVLEVQQQ
                     LGDGVVRTIAMGSTEGLKRGLAVNSTNAPISVPVGLETLGRIMDVLGNPIDECGPIGE
                     KERMPIHRKPPTYAEQAAGNELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIATEHSGLSVFAGVGERTREGNDFYHEMQESGVVNTKTFSDSKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPT
                     LAEEMGALQERITSTKTGSITSIQAVYVPADDLTDPSPATTFSHLDATVVLSRDIAAK
                     GIYPAIDPLDSTSRQLDPLVIGSEHYEVARGVQT"
     gene            complement(1193..2053)
                     /gene="atpG"
                     /locus_tag="VFX11_11690"
     CDS             complement(1193..2053)
                     /gene="atpG"
                     /locus_tag="VFX11_11690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX4939330.1"
                     /translation="MAGAKEIRTKIASVKNTQKITSAMQLVAASKMRKVQERMAASKP
                     YAEKMRDVIHHLANSNPEYRHTYMTERDVKRIGVIVVSTDRGLCGGLNINLFKRVVAL
                     GKEWKDKGAELELALIGTKAVSFFKSVGGNVVATKTGLGDYPTVEQLIGAVKVAFDSF
                     EQGKIDRLFLVSNEFVNTMTQKPVARQLLPLEASDDPAYKHRWDYIYEPEAKETLDFL
                     LVRFLESQVYQGVIENNACEQAARMVAMKSATDNAGNLIKELQLIYNKARQAAITQEI
                     SEIVGGAAAV"
     gene            complement(2108..>2767)
                     /locus_tag="VFX11_11695"
     CDS             complement(2108..>2767)
                     /locus_tag="VFX11_11695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072124.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4939331.1"
                     /translation="SRLLERAARVNAEYVEKFTNGAVKGKTGSLTALPIIETQAGDVS
                     AFVPTNVISITDGQIFLETSLFNSGIRPAMNAGISVSRVGGAAQTKIIKKLGGGIRLA
                     LAQYRELAAFAQFASDLDEATRKQLEHGQRVTELMKQKQYSPLSVAEMGLVLYVANEG
                     YLADVEIKKVLDFEAALLSYANSEYATLLAKVNEKGDWSNEIEAQYKALVEKFKSTQT
                     W"
BASE COUNT          519 a          821 c          796 g          631 t
ORIGIN      
        1 ggtctgcacg ccgcgagcca cttcgtaatg ttcggaaccg attaccagcg gatccagctg
       61 acggctggtg gaatccagcg ggtcgatcgc cgggtagata cccttggcgg cgatgtcacg
      121 gctcagtacc acggtggcgt caaggtggga gaaggtggtg gccggagacg ggtcggtcaa
      181 gtcgtccgcc ggtacgtata ccgcctggat agacgtgatg gagcccgtct tggtggaggt
      241 gatgcgctcc tgcagggcgc ccatctcttc cgccagagtc ggctgataac ccactgcgga
      301 tggcatacga cccagcagtg cagacacttc ggtgcccgcc agggtgtaac ggtaaatgtt
      361 gtcaacgaac agcagaacat cacggccttc atcacggaat ttttccgcca tggtcagacc
      421 ggtcagggcc acacgcagac ggttgccggg aggctcgttc atctggccgt acaccatcgc
      481 taccttggaa tcgctgaagg tcttggtgtt aacaacgccg gattcctgca tttcgtggta
      541 gaagtcgttc ccttcgcggg tccgctcacc cacaccggca aacacggaca gaccggagtg
      601 ctcggtggcg atgttgttga tcagttccat catgtttacg gttttgccta caccggcacc
      661 accgaacaga ccgactttac cgcccttggc gaacggacat accaggtcga taaccttgat
      721 gccggtttcc agcagttcgt tgcccgctgc ctgctcagca taggttgggg gcttgcggtg
      781 gatcggcatg cgttcttttt cgccgatggg gccgcactcg tcgatcgggt tgcccagcac
      841 gtccatgatg cggccgaggg tttcaaggcc caccggtacg ctgatcggcg cgttggtgct
      901 gttcaccgcc agaccgcgct tcaggccttc ggtactgccc atcgcgatgg tgcgcaccac
      961 gccgtcgccc agctgctgct gcacttccag caccgtggtc acggtgtcca ctttcagcgc
     1021 gtcgtacacg ttgggtacgg aatcgcgcgg aaattcgacg tcgataaccg cgccgatgat
     1081 ttgaacgata cgtccgctac tcatgttgcc atcctcttaa accagaaatt gacggggctc
     1141 tttcgcaatc actgtccgca atcagggccc aaaactgttc attaccggaa gcttatactg
     1201 cggcggcgcc gcccacgatc tcggaaattt cctgcgtgat cgcggcctgg cgggccttgt
     1261 tgtagatcaa ctgcagttcc ttgatcagat tgccggcgtt gtccgtggca gacttcatgg
     1321 ccaccatccg ggctgcctgt tcacaggcat tgttctcgat cacgccttgg taaacctggg
     1381 attcaaggaa gcgaaccagc agaaaatcca gggtttcctt ggcttccggc tcgtaaatgt
     1441 aatcccagcg gtgcttgtag gcgggatcat ctgacgcttc cagcggcagc aactgccggg
     1501 ccaccggttt ctgggtcatg gtgttgacga attcgttgga caccaggaac agacggtcga
     1561 tcttgccctg ttcgaaggaa tcaaaagcaa ccttcaccgc gccgatcagc tgctccacgg
     1621 tagggtagtc ccccagtccg gtcttggtgg ccacgacatt gccaccgaca gacttgaaga
     1681 aactcacggc cttggtgccg atcagggcca gctcgagctc tgcgcccttg tccttccact
     1741 ccttgcccag cgcgacgact cgcttgaaca ggttgatgtt caggccaccg cacaatccac
     1801 gatcagtgga taccacaatc acgccgatcc gctttacatc acgctcggtc atgtaggtgt
     1861 gacggtactc cggattggaa ttggccaggt gatggattac atcgcgcatc ttttccgcat
     1921 agggcttgga cgcagccatg cgctcctgca ccttacgcat tttgctggca gccaccagct
     1981 gcatcgcaga ggtaatcttc tgggtgttct tcacactggc aattttggtg cgtatttctt
     2041 ttgcgcctgc cataggtctt accgtatcca ggttacggag cggccgttgc gccgctccgg
     2101 tggaggatta ccaagtctgg gtggacttga acttctcaac cagcgccttg tattgcgctt
     2161 cgatctcgtt gctccagtcg cccttctcgt tgaccttggc cagcagagtg gcgtattcgc
     2221 tgttggcgta ggacagcagg gccgcttcga aatccagaac tttcttgatt tcgacgtctg
     2281 ccagatagcc ttcgttggcc acgtacagaa ccagacccat ttccgcaaca gacagcggcg
     2341 agtactgttt ctgcttcatc agctcggtaa cgcgctgacc gtgttccagc tgcttgcggg
     2401 tcgcttcgtc caggtcggaa gcgaactggg caaacgcggc cagttcccgg tactgcgcca
     2461 gggccagacg gataccgccg cccagtttct tgatgatctt ggtctgcgct gcaccaccta
     2521 cccgggatac cgagataccg gcgttcatcg cgggacggat accgctgttg aacaggctgg
     2581 tttccaggaa gatctggccg tcggtgatgg agattacgtt ggtcggaacg aacgcggata
     2641 cgtcaccggc ctgggtttcg atgatcggca gcgcggtcag tgaaccggtc ttgcctttta
     2701 ccgcgccgtt ggtgaacttc tcaacatact cggcattaac gcgggctgca cgctccagca
     2761 gacggga
//
LOCUS       DASUEG010000621         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2190466, whole genome shotgun sequence.
ACCESSION   DASUEG010000621 DASUEG010000000
VERSION     DASUEG010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190466"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /gene="mdoH"
                     /locus_tag="VFX11_11700"
     CDS             <1..1959
                     /gene="mdoH"
                     /locus_tag="VFX11_11700"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX4939332.1"
                     /translation="VLPYHGSDWLELSLAVLFAILFTWISIGFWLGLYGFVLRRFGGD
                     SLSLLRRHSKFLDQTPMARTAVVMPIYNEPIERTLGGLRAVYRSLQATGQSENFDFFI
                     LSDSRDPDIWLSEQATWYQLCRELGAEGHLFYRRRSLNMHYKSGNIADFLRRWGRNYE
                     YTIVLDADSLMEGDTLVKMVKLMQREPNVGILQTGPSLINGKSLFARVQQFSNQLYGP
                     LFSTGLAALQLGDAAYWGHNAIIRTELFMRHCGLRTLGGPGIFGGPILSHDFVEAAYM
                     GRAGHEVWLEPALEGTYEESPPTVVDELTRDKRWAKGNIQHLWLLLFEPRLRFAHRMA
                     FLNGLMSYCASPLWLAFLVLSTFEVTRLILWPINYFPAEYSLFPVWPEFHPELALALA
                     SSTIGLLFVPKVLAFLDALMTRGRTRKFGGFFQLLRGMLLEILVSILLAPIRMLAHTR
                     YVIEALFNLNLSWAGQNRTQETRWWDAIVNQAPGTILALGWSGFAYWLDEMFFYWSLP
                     VALPLILAAPISVLTGKIGIGLLLEKWGFLRIPEEVNGSKLVDELERMPVTDAQLPRS
                     AFVQAVVDPVMNHVHCTLARGHRGGVKQSRLAVLRRRCLKDGPESLSGKEISMLAKDK
                     ESLRWLHEEAWRASHDSYWGRVITARSY"
BASE COUNT          360 a          660 c          642 g          420 t
ORIGIN      
        1 gtcttgccct accacggcag tgactggctg gaactgagcc tggcggtact gtttgccatt
       61 cttttcacct ggatctccat cggcttctgg ctgggactgt acggcttcgt gctgcgtcgc
      121 ttcggcggcg actccctgtc actgctgcgc cggcacagca aatttctgga ccagacaccc
      181 atggcgcgca ccgccgtggt catgcccatc tacaacgaac ccatcgaacg cactctgggc
      241 ggcctgcgag ccgtgtaccg ttcactgcag gcaacagggc aaagcgagaa cttcgacttt
      301 ttcatcctgt cggacagccg tgacccggat atctggctgt cggagcaggc cacctggtac
      361 cagctgtgcc gggaactggg cgccgaaggc cacctgttct accgccgccg ctccctgaac
      421 atgcactaca aaagcggcaa cattgccgat ttcctgcgcc gctggggccg caattacgaa
      481 tacaccatcg tgctggacgc cgacagcctg atggaaggcg acaccctggt gaaaatggtg
      541 aagctgatgc agcgcgagcc caatgtgggc atcctgcaga ccggccccag cctgatcaat
      601 ggcaaatccc tgtttgcccg ggtacagcag ttctccaacc agctgtatgg cccgctgttc
      661 agcaccggac tcgccgccct gcaactgggt gacgccgcct actggggtca caacgccatc
      721 atccgcacag agctgttcat gcgccattgc ggcctgcgca ctctgggtgg cccaggcatt
      781 tttggcggcc cgatcctgag ccatgatttt gtcgaagccg cctacatggg ccgcgccggc
      841 catgaagtct ggctggagcc ggcgctggaa ggcacctacg aggaatcgcc gcccaccgtg
      901 gtggacgaac tgacccgcga caaacgctgg gccaagggca acatccagca cctgtggctg
      961 ctgctgttcg aaccccgcct gcgtttcgcc caccgcatgg cgttcctgaa cggcctgatg
     1021 tcctattgcg catcgccgct gtggctggca tttctggtgc tgtccacctt cgaggtcacc
     1081 cgcctgatcc tgtggcccat caactatttt cccgccgagt acagcctgtt cccggtgtgg
     1141 ccggagttcc atccggaact ggcgctggca ctggcgtcga gcaccatcgg attactgttc
     1201 gtgcccaagg tgctggcttt tctggatgca ctgatgaccc ggggccgcac ccgcaagttc
     1261 ggcggattct tccagctgct gcggggcatg ctgctggaga tcctggtgtc gatcctgctg
     1321 gcgccaatcc gcatgctcgc ccacacccgc tatgtgatcg aggcgctgtt caacctgaac
     1381 ctgagctggg ccggccagaa ccgtacccag gaaacccgct ggtgggacgc cattgtcaac
     1441 caggcgccgg gtacgatcct ggcgctgggc tggagcggtt ttgcctactg gctggacgaa
     1501 atgttctttt actggtcgct gccggtggca ttgccgctga tcctggcagc accgatttcc
     1561 gtgctgaccg gcaagatcgg catcggcctg ctgctggaaa aatggggctt tctgcggatt
     1621 cccgaggaag tgaatggcag caagctggtg gatgaactgg aacgcatgcc ggtgaccgat
     1681 gcccagctgc cgcgctcggc cttcgtgcag gcggtggtgg acccggtgat gaaccacgtg
     1741 cactgcacgc tggctcgcgg acatcgcggc ggcgtgaagc agagccggct ggctgtgttg
     1801 cgtcgccgct gcctgaagga cggcccggag agtctgtcgg gcaaggaaat cagcatgctg
     1861 gcgaaggaca aggagtcact gcgctggctg catgaggaag cctggcgcgc gtcgcacgac
     1921 agttactggg gccgtgtgat cacggcgcgc agttactgag aacggatgat ccagcgggga
     1981 ctcgcaacgg gttcccgtat ttgagccgtc cccattttca gcccctctgt tgttcagcgt
     2041 cggtccgctc ctgtcagaca ggagcggccg atcggctttt ca
//
LOCUS       DASUEG010000622         5300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1993999, whole genome shotgun sequence.
ACCESSION   DASUEG010000622 DASUEG010000000
VERSION     DASUEG010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993999"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..89
                     /locus_tag="VFX11_11705"
     CDS             3..89
                     /locus_tag="VFX11_11705"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX4939333.1"
                     /translation="MHPKVYLPIEKTGHAACPYCGAQYKLVG"
     gene            106..1131
                     /gene="waaF"
                     /locus_tag="VFX11_11710"
     CDS             106..1131
                     /gene="waaF"
                     /locus_tag="VFX11_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX4939334.1"
                     /translation="MPKQILIVGPSWVGDMVMAQVLFKLLRQQLPDCAIDVLAPAWSR
                     PLLVRMPEVRAAIDLPFGHGEFAFGKRKALGRALAATQYDQAIVLPNSWKSGLVAYFA
                     RIPQRTGWRGEMRYGLLNDIRPLDKARYPLMIERFAALAFARDAKLPEKLPWPAFSVR
                     DDDIASALAAMQIERPTTPVLALCPGAEFGPAKRWPDRHYAAVAKHFLDQGWQVWLFG
                     SAKDRDVTELINSACDNRCVDLAGRTSLAQAIDLMSLATQVVSNDSGLMHVAAALKRP
                     LVVPYGSSSPKFTPPLSEQVRVLQLDLDCSPCFKRECPLGHLNCLNQLEPSQVLNAIR
                     ALQPRLL"
     gene            1140..1964
                     /gene="rfaP"
                     /locus_tag="VFX11_11715"
     CDS             1140..1964
                     /gene="rfaP"
                     /locus_tag="VFX11_11715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HEX4939335.1"
                     /translation="MSEFYLREDLHAALAPANRFDDILALQGKVFRELDGRRTLQFER
                     GGKSYFAKLHFGIGWAEVFKNLLSGRLPIVSARNEWLAIRHLEQHDIGTMTLAGYGER
                     GRNPAKIESFVLTDELTDTVSLETLALEWAQQPPSPRFRRAIIHELARVAGRMHRSGM
                     NHRDFYLCHFLLPNPQREQSPVRLHLIDLHRAQIRAKTPYRWQLKDIAGLYFSAMDAG
                     FNRRHLLRFVRLYEQCSLRQLTGEKMRFWRQVEKRACALYEKVHNRQPPSHFYPQP"
     gene            1966..3105
                     /locus_tag="VFX11_11720"
     CDS             1966..3105
                     /locus_tag="VFX11_11720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4939336.1"
                     /translation="MSTNLTLALVLPEYFPYGGKQRDFLKIATAAVAAGHQVRALVGS
                     WEGAVPEGIIVERLAVSGRTNHRRLICFANAVKANLSNNPADIVIGFTKIPGLDWYFA
                     ADTCYRARIQRRYGGWLDWLPRVRTLSSFESAVFANTSTTRILSLNPRERDVYRTLYA
                     SPNNRFVNLPPGVTEAFQVPTADQRKRLRESAGIADSDFLLLMVGSHFMTKGVDRSIA
                     ALAALPEALKKRCQLWVVGKGKDTPMRRLVDRHELDSQVKFLGGRDDVPTLMQTADLL
                     LQPSRTELAGMSIVEGLASGLPVIASGVCGYSTHVMASNAGCVLMEPFCQRDLVLAMQ
                     LLLHPTELESARKNAAAYAEKNNLRALAINALDAIVASRSIPIGG"
     gene            complement(3102..4280)
                     /locus_tag="VFX11_11725"
     CDS             complement(3102..4280)
                     /locus_tag="VFX11_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEX4939337.1"
                     /translation="MNVTELMLALPIMVLGRLLRRDRNSWSIIVDKRQGFEGNLLAIA
                     AHLAKRKGTTVYVIDCGNRGIGVDEALGENVKFIRHHSFAELKARLRSRQVVVTHSLK
                     PALGRYSLLNAVRIINVWHGIPVKGMGNMDQSFPRNRLAVEFKRRKAISIFCVSSELE
                     RTFIAACHLMDASRIHVLGIPRTDMLIDQSVMTSGQCRIHSAISAVKSGRKMVLYAPT
                     FREEAADSLGLTRADMERLNVLFADHDVLFAVRPHPKEAAAYDQLVAGLSHIVHADSR
                     RWPDTNTVLLQSDALVTDYSSIWLEYLLLNRPLAAFWWDFQKYADHRGFLIDLEAIFP
                     GPKAFTVPELEQCFRRWLSHGFANEPEWGLLQQQMRHLFHKHADGRSSERVVNAATLD
                     "
     gene            complement(4277..4699)
                     /locus_tag="VFX11_11730"
     CDS             complement(4277..4699)
                     /locus_tag="VFX11_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX4939338.1"
                     /translation="MAVKKNDKERKVRVVTFGSFDLFHIGHLNILKRARTLGDELYVG
                     LSSDALSEQKKGRKPVYSFQHRRQILESLRVVDFVFEEESLDKKLDYLQRYQADLLVM
                     GDDWLGRFDFCKACCDVVYLPRTPSISTTELIEIIREK"
     gene            complement(4765..>5300)
                     /locus_tag="VFX11_11735"
     CDS             complement(4765..>5300)
                     /locus_tag="VFX11_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4939339.1"
                     /translation="AHMLLSVGSGFRIKGLDRTLTALSRLPAELRANTHLFVVGQDKA
                     RGFLRQANALGLTTVHFLGGRNDVAELMVAADLLLHPAYSENTGTVLLEAVTSGLPVI
                     CTARCGYAAHVQAANAGVVLSEPFSLDRYMLEVMRLLRNAQERKHLAEQGRTFAAQAD
                     WFSMPERAAQLLLDSLV"
BASE COUNT         1166 a         1570 c         1416 g         1148 t
ORIGIN      
        1 acatgcaccc gaaggtctac ctgccgatcg aaaagaccgg ccacgcggcc tgcccctact
       61 gcggtgcgca gtacaagctc gttggctgag ccggacgccc tccaattgcc aaaacagatt
      121 ctgatcgtcg gcccgtcctg ggttggcgac atggtgatgg cgcaagtgct gttcaaactg
      181 ctgcgccagc aactgcccga ttgcgccatt gatgtgctgg caccggcctg gagccggccg
      241 ctgctcgtgc gcatgccgga agtgcgcgcc gccatcgatt tgccgttcgg ccacggcgag
      301 tttgcgtttg gcaagcgcaa ggcgcttggc cgcgccctcg cggcgacgca atacgatcaa
      361 gccatcgtgt tgccgaactc gtggaaatcg ggtctcgttg cctatttcgc ccggatcccg
      421 cagcgcaccg gttggcgcgg cgaaatgcgt tatggcctgc tgaacgatat ccggccactc
      481 gacaaagcgc gctacccgct gatgatcgag cgctttgccg cactcgcctt cgcgcgcgat
      541 gccaaactgc cggaaaagct gccatggcca gcgttcagcg tgcgcgacga cgatatcgcc
      601 agcgcgctcg ccgcgatgca gatcgagcgg cccacgacgc cggtgctggc gctctgcccg
      661 ggcgccgaat tcggcccggc caaacgctgg ccggatcggc attacgccgc agtggcgaag
      721 catttccttg atcaaggttg gcaagtctgg ctgttcggtt cggcgaaaga tcgcgacgtc
      781 accgagctca tcaatagcgc ctgcgacaac cgctgcgtcg acctggctgg ccgcacctcg
      841 ctggcacagg ccattgatct gatgtcactg gcgacccagg tggtgtcgaa tgattccggg
      901 ctgatgcatg tcgccgccgc gctgaaacgg cccttggtcg tgccgtacgg ttcatcgtca
      961 cccaaattca cgccaccgct cagcgaacag gtgcgcgtgt tgcaactcga tctggattgc
     1021 agcccctgct tcaagcgcga gtgtccactc gggcatttga actgcctgaa tcagctggaa
     1081 ccgagtcagg tgctgaatgc gatccgcgcc ttgcagccac ggctgctgtg atcacggcga
     1141 tgagcgagtt ctatctgcgc gaggatctgc acgcagcgtt ggcgccggca aaccgcttcg
     1201 acgacatcct ggcactgcaa ggcaaggtat ttcgtgagct ggacggtcgc cgcacgctgc
     1261 agttcgagcg cggcggcaaa tcctattttg ccaaactgca tttcggcatc ggctgggcgg
     1321 aggtgttcaa gaacctgctc agcggccgcc tgcccatcgt cagtgcccgc aatgaatggc
     1381 tggcgatccg ccatctggaa cagcacgaca tcggcacgat gacgctggcc ggttacggcg
     1441 aacgcggtcg caacccggcg aaaatcgaat cgttcgtgct gaccgatgaa ctgaccgata
     1501 ccgtgtcact ggaaacgctg gcgctggaat gggcgcagca accgccgtca ccacgctttc
     1561 gccgcgccat catccatgaa ctggcacgcg ttgccggccg catgcaccgc agcggcatga
     1621 accaccgcga cttctacctc tgccatttcc tgttgccgaa tccgcagcgc gaacagtcac
     1681 cggtgcgcct gcatctcatc gacctgcacc gcgcccagat ccgggccaag acgccctacc
     1741 gctggcagtt gaaagacatc gccggcctgt atttttcggc gatggacgct ggcttcaatc
     1801 gtcggcattt gctgcggttt gtcaggttgt acgaacagtg ttcacttcgc cagcttactg
     1861 gtgaaaaaat gcggttctgg cggcaggttg agaagcgcgc ctgtgcgctt tatgagaaag
     1921 tacacaatcg acagccacct tcacatttct acccacaacc ctgacatgtc gaccaatctg
     1981 acgcttgctc ttgtactgcc ggaatatttt ccttacggag gaaagcagcg tgatttcctg
     2041 aaaatcgcga cagcggctgt cgcggccggt caccaggttc gcgccttggt tgggagctgg
     2101 gagggggcag tgccggaggg catcattgtc gagcgtcttg cagtttccgg ccggaccaat
     2161 catcgccgac tgatttgctt tgctaatgcc gttaaggcga acctgagcaa taatccagct
     2221 gatatcgtga ttggatttac caaaattccc ggtctcgact ggtacttcgc cgcggacaca
     2281 tgttatcgag cacgcattca gcgtcgatat ggcggctggc tggactggct gcctcgtgtt
     2341 cgtacgctct cctcgttcga aagcgctgtt tttgccaata cctcgacgac gcgcatcctc
     2401 agcctcaatc ctcgcgaacg cgatgtgtac cgtaccttat atgcttcgcc aaataatcga
     2461 tttgtcaatt tgcctcctgg ggtcacggaa gcctttcaag taccaacagc agaccagcgc
     2521 aaacgactgc gcgaatcggc gggaattgcg gacagtgatt ttctgttgct gatggtcgga
     2581 agtcacttca tgaccaaagg agtggaccgc agcattgcgg ctcttgctgc cttgccggaa
     2641 gcactgaaaa aaaggtgcca gctgtgggtt gtaggaaagg gcaaggacac ccccatgcgc
     2701 cgcttggtgg accggcacga gctggattcg caggtcaaat tccttggcgg ccgtgacgat
     2761 gtcccaacat tgatgcagac agcagatctg ctgttgcagc cttcgcgaac agaattggcg
     2821 ggcatgagca ttgttgaagg gttagcttct ggcttaccgg ttattgccag cggagtgtgt
     2881 ggctattcga cacacgtgat ggccagcaat gccggctgcg ttctgatgga gccgttttgt
     2941 caacgggatt tggttctggc catgcagttg ctattgcacc cgacggaact ggaatcggcc
     3001 agaaagaacg ctgctgcata cgccgagaaa aataatctac gcgctctggc aattaacgca
     3061 cttgatgcga ttgtcgccag cagatcgata cctattggtg gttaatccag cgtcgccgca
     3121 tttacgactc tctcgctgct tcggccatca gcatgtttat gaaaaaggtg ccgcatctgc
     3181 tgctgtagca gtccccactc cggctcattc gcaaaaccgt ggctcagcca tcgccgaaag
     3241 cattgctcaa gctctggcac cgtaaaggcc ttgggaccgg gaaaaatggc ttcgagatcg
     3301 atcagaaaac cacggtggtc tgcatatttc tgaaaatccc accaaaatgc agcaagtggt
     3361 cgattcaaca acaagtactc gagccaaatg ctgctgtagt cggtaaccaa cgcatctgac
     3421 tgcaacaaaa ccgtattcgt atccggccat cgtctgctat ctgcatggac aatatggctc
     3481 aatccagcga ccaattggtc gtaggcggca gcctctttcg gatgaggccg taccgcaaat
     3541 agcacatcat ggtcagcaaa caggacatta aggcgctcca tgtcagcacg cgtcaacccc
     3601 aaagaatctg ccgcctcttc ccgaaaggtt ggcgcataga ggaccatttt tcggcctgat
     3661 ttgacagcag agattgcgct gtgaattcga cactgaccgc ttgtcatcac gctttggtcg
     3721 atcagcatat cggtacgagg aatcccaagc acatgaatgc ggctcgcatc catcaaatga
     3781 caggcggcaa tgaaagtacg ttcaagctca gaggagacac agaaaattga aatggctttt
     3841 cgccgcttga actccactgc gagtcgattg cgaggaaagc tttggtccat attgcccatg
     3901 cctttgacag gaatgccgtg ccagacattg atgatccgga ccgcatttag caaagagtaa
     3961 cgccctagcg ctggcttcag gctgtgcgtg acaacgactt ggcggctacg caatcgcgcc
     4021 ttcagctccg caaaagagtg atgtctaatg aactttacat tttctcccag cgcttcatca
     4081 acaccaatac cgcgatttcc gcagtcaatg acgtaaactg ttgtgccttt tctcttggcg
     4141 agatgagctg caattgccag cagattccct tcgaaaccct gccttttatc gacaatgatt
     4201 gaccacgaat tcctgtctcg ccggagcagc ctccctagca ccatgatggg aagtgcaagc
     4261 atcagttcag taacgttcat ttttcgcgaa tgatctcgat cagctcagtg gtcgatatgg
     4321 acggtgttcg cggcagataa accacatcac agcaggcttt gcagaaatcg aaccggccca
     4381 accaatcatc acccataacc agaagatctg cctgataacg ttgtaagtag tccagctttt
     4441 tgtccagact ttcttcttca aaaacgaaat caaccacccg cagcgactct agtatctggc
     4501 gtcggtgctg gaaactgtag accggcttgc gccccttctt ttgctcactg agcgcgtcag
     4561 atgacagccc gacatacaac tcatcaccaa gcgttctagc gcgcttcaat atattcagat
     4621 gaccgatatg aaacagatca aaactgccaa aagtgaccac acgaactttt ctttctttat
     4681 cgtttttctt caccgccatc gcattccttt tcgagcccgg ctgattgagg ccagacccca
     4741 acgcacttga tgagacagca tcgactagac aagactgtcg agcaatagct gcgctgcccg
     4801 ttccggcatt gagaaccaat cagcctgcgc ggcaaatgtc cgcccctgtt cagcaaggtg
     4861 tttccgctcc tgcgcattgc gcaacagccg catcacctcc agcatatagc gatcaagcga
     4921 gaacggctcc gacagtacca cgccggcatt ggcggcttga acatgcgccg catagccaca
     4981 gcgtgcagta catatcaccg gcaaaccact tgtcacggcc tctagcagca ccgtaccggt
     5041 attttcacta taggcagggt gtagcagcag gtcggctgcg accatcagct ccgcaacgtc
     5101 attccttcct cccagaaagt gcacagtcgt caatcccaac gcgtttgctt gccgcaaaaa
     5161 gccccgggct ttgtcctgcc caacaacgaa aagatgtgtg ttcgccctca actctgccgg
     5221 caagcgactc agtgcagtca gggttctatc aaggcccttg atgcgaaagc cggaccctac
     5281 cgacagcagc atgtgtgcag
//
LOCUS       DASUEG010000623         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1892877, whole genome shotgun sequence.
ACCESSION   DASUEG010000623 DASUEG010000000
VERSION     DASUEG010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892877"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..913)
                     /locus_tag="VFX11_11740"
     CDS             complement(353..913)
                     /locus_tag="VFX11_11740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX4939340.1"
                     /translation="MNFLLLSGSFHSRSRSLVIIDTLRPFLSGHAVQVPQLDRLPFYS
                     DDLNQNKPPVVQDFLAQVTAADAIICCTPEYNHSIPAVLKNAIDWASRPAFASPLKGK
                     PVSIITQAVSAVGGARAQAHLKLVLDSTLSEIHPCHEMMITGIDALTDGKSPITERKV
                     LERLERHLTDFIRYAQRRAAVSHPSP"
     gene            complement(964..1527)
                     /locus_tag="VFX11_11745"
     CDS             complement(964..1527)
                     /locus_tag="VFX11_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEX4939341.1"
                     /translation="MLSPINHGRRRFVRLSAGAALAAPLLASMGARAADARDLQLVSL
                     DAALNELNRLAQAGALDSSAVWTWAQTLTHCAQSIEFSMTGFPEAKSALFQSTVGSAA
                     YAVFAWRGRMSHDLAEPIPGAASLAGVTDNVAAETRLRAAILAFQQWPGELQPHFAYG
                     ALSKPEYELAHAMHLANHFSQFNVRSA"
     gene            complement(1607..2203)
                     /locus_tag="VFX11_11750"
     CDS             complement(1607..2203)
                     /locus_tag="VFX11_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HEX4939342.1"
                     /translation="MSHPIQCSCGAVKGNLSNTRDINRCVCYCRDCQAFAHFLGRQTD
                     ILDSRGGSDIVQTLPKYVSFSAGTEHLACMRLTDKGLLRWYASCCNTPIGNTAPNFSM
                     SFVGLVHNCLEAGGASMDAVFGPVSAHVNTENARGGVRVKSKGFAKTLLKVLGMVARA
                     RIDGSYRKTPFFNVEKGMPIVTPTILNAQERLQLMDGL"
     gene            complement(2257..2784)
                     /locus_tag="VFX11_11755"
     CDS             complement(2257..2784)
                     /locus_tag="VFX11_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4939343.1"
                     /translation="MATEKNAGDITRLIQLSARQLGRSDYSSAQIEAALNSAWGLDSQ
                     LIADKTYFQVMAGRTLVGCGGWSFRRTLFGSDSRGDRDSSPLDPAKDAAKIRAFFIHP
                     DHARQGLGTRLLIHCEQQAWDSGFTRLELGATLPGVRLYSTLGYVAGEAYDFPTGPDT
                     VLAIIPMSKILRCRP"
     gene            complement(3154..>3528)
                     /locus_tag="VFX11_11760"
     CDS             complement(3154..>3528)
                     /locus_tag="VFX11_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939344.1"
                     /translation="DKVVLDLYVGLAAALKPKGSAQSPDVQVDVGAAKAAAGDRPGML
                     MANAVTADVRVESVDLKNNKIRVTGPSGVVRELDVRHPDFQAMLKGLKTGDIVEMTYF
                     EAVAVGVEPAAAAKAETTGKSY"
BASE COUNT          769 a         1062 c         1003 g          694 t
ORIGIN      
        1 tttcttccgc ggtggcggcc gaagcttcgt ggatggggga atacagcgct gctgcagcac
       61 cggtggcggc gctggccttc aaaaagtcgc gacgtttcat tgtatttgtt atccgttggt
      121 cggtgtgaaa ctcgctacag ccagcaagac tggcaagcga acaaacatgc cgacatcaag
      181 tcgacacttg caacggatgc taaaggaacc cacagatttt cgattgtttt aatggggttg
      241 tttttatcaa tcgctggcca aatcaaacca ttcgcaaaac cggcttgcag cactaccaac
      301 ataaagggac aaaaagcaaa tcagatggcc agatcaaacg caacaaccag tgtcacggtg
      361 acgggtggga tacggccgcg cggcgttgcg cataacggat gaaatcagtt aggtgtcgct
      421 ccagccgttc cagcaccttg cgttccgtga ttggcgattt tccgtcggtc agtgcatcaa
      481 ttccggtgat catcatttca tggcagggat gaatttctga cagcgtggaa tcgagcacca
      541 gtttgagatg ggcctgcgcc cgcgcgcccc cgacagcact gacggcctgg gtgatgatgg
      601 acaccggctt gcccttgagc ggcgacgcaa acgccggccg cgacgcccag tcaatcgcat
      661 ttttcagaac cgccggaatg ctgtgattgt attccggcgt gcagcagatg atggcatcgg
      721 ccgcggtcac ctgcgccagg aaatcctgca ccaccggtgg cttgttctgg ttcaggtcgt
      781 cgctgtaaaa cggcagccgg tccagttgcg gcacctgcac ggcatggcca gacaggaatg
      841 gccggagggt gtcgatgatc accagacttc tgcttctgga atggaaactg ccggacagca
      901 acagaaaatt catgattacc tcagcaggcc aatgggtgcc agcaggcttt gcgcaacctc
      961 atgctacgcg gacctgacat tgaactggga aaaatgattg gccaggtgca tggcatgggc
     1021 aagctcatat tccggcttgc tcaacgcgcc ataggcgaag tgcggttgca gttcccccgg
     1081 ccactgctga aacgccagga tagcggcacg caggcgggtt tcggcagcca cgttgtcagt
     1141 cactcccgcc agcgaagccg cgccgggaat cggctcggcc agatcgtgcg acatgcgtcc
     1201 acgccaggca aacaccgcat aggcagccga accgaccgtg ctctgaaaca gcgcggattt
     1261 cgcttcggga aaacccgtca tggagaattc gatgctttgc gcgcaatggg tcagcgtctg
     1321 cgcccaggtc cagactgcgc tggaatccag agcgcctgcc tgcgccagcc ggttcaactc
     1381 attcaatgct gcatccagcg acaccagttg caggtcgcgc gcgtcggcgg cgcgggcacc
     1441 catactcgcc agcagcggcg cggccagtgc ggcacctgcg gacaacctta caaaacggcg
     1501 gcggccatga ttgattggcg atagcacccg gtttctctcc cagtcaatgt cgaatgaaat
     1561 ggtttggacc tgaacagcag gacatgccgg cattgtcgcc cggcacttac agtccgtcca
     1621 taagctgcag ccgttcctgt gcgttcaata ttgttggcgt cacgatcggc atgccttttt
     1681 ctacgttgaa gaacggcgtc ttccggtaac tgccgtcgat gcgcgcacgc gccaccatac
     1741 ccagcacctt cagaagggtt ttggcaaacc ccttcgattt aacgcggaca ccgccccgtg
     1801 cattttcagt attcacgtgg gcgctcaccg ggccgaagac tgcgtccatc gaagccccgc
     1861 ccgcctccag gcagttgtgc accagcccga cgaacgacat gctgaaattt ggcgccgtat
     1921 tgccgatcgg tgtattgcaa cagctggcat accagcgcag cagccccttg tcagtcaggc
     1981 gcatgcaggc cagatgttcg gtaccggcgc tgaagctgac atatttcggc aacgtctgca
     2041 cgatgtcgga gccgccacga ctgtccagaa tgtcggtctg ccgcccgaga aaatgcgcga
     2101 acgcctggca gtcgcggcag tagcagacgc agcggttgat gtcgcgggta ttgctgaggt
     2161 tacctttgac ggcaccgcag gaacactgga tcggatggct catcggagca gctcctgaat
     2221 gaatgcgcct gccctgccgg ccagggcggc accggatcac gggcggcaac gcaagatttt
     2281 tgacatgggg atgatggcga gaaccgtatc gggcccggtt ggaaaatcat aggcctcacc
     2341 cgccacgtaa ccgagggtac tgtacagccg gacaccgggt aaagtggcgc ccagttcaag
     2401 gcgggtaaat ccggaatccc aggcctgctg ttcgcagtgg atcagcaacc gggtgcccag
     2461 gccctggcgg gcatgatcag gatgaataaa gaaagcacgg attttggcag catcctttgc
     2521 cgggtccagc gggctgctgt cccggtcgcc ccgggaatcg ctgccgaaca gggttcgcct
     2581 gaaactccag ccgccacatc ccaccagcgt gcggccggcc atgacctgaa aataggtttt
     2641 atccgcaatc agttgcgaat ccaggcccca ggcactgttc aaagcggctt cgatttgcgc
     2701 ggaagaataa tcggagcggc ccagctgacg agccgaaagc tggatcagcc gggtaatgtc
     2761 gcctgcattt ttttcagttg ccaattgaat tgagtattgc accaaccacc atcattactg
     2821 ttcagcacgt gcagtcaatt catgaaagaa acaccattca gccggacacg tcaaccgtat
     2881 tccaatgacg ttaacgggcc tgccgacgcg cctgaatcct gtcagccatc agcaccagca
     2941 ggcccaccca tcccagcagg ccgaaaaaca gcgcagtgcc cactgcactc aggctgatga
     3001 tccagggtaa tcaggggaca tccggtattc gccaccggat gcaggatgtg cggatgatgg
     3061 tagtgtttcg ctgttcatga tcgccgtcct ctcgttccgg cgatcatgaa caggaacatt
     3121 caactcaaca aatgataatt ccttgccaac ggattagtac gacttaccgg tggtttctgc
     3181 cttggcagcc gctgccggtt ccacgcccac ggcaaccgct tcgaaatagg tcatctcgac
     3241 gatatcgccg gtcttgagtc ccttcagcat ggcctgaaag tcgggatgcc gcacatccag
     3301 ctcgcgcacc acaccggaag gcccggtcac ccggatttta ttgttcttca gatcgaccga
     3361 ttccacccgc acatcggcgg taaccgcatt ggccatcagc atgccagggc gatcaccggc
     3421 cgccgccttg gcggcaccga cgtccacctg cacatcgggg gattgcgcgc tgcccttggg
     3481 cttcagcgcc gccgccagac ccacatacag atccagcacc accttgtc
//
LOCUS       DASUEG010000624         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_954089, whole genome shotgun sequence.
ACCESSION   DASUEG010000624 DASUEG010000000
VERSION     DASUEG010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954089"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VFX11_11765"
     CDS             complement(<1..1448)
                     /locus_tag="VFX11_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX4939345.1"
                     /translation="MARLTEYSSYADAQAHASSAALWDLFDGDREHLNIAHECITRHA
                     DGSGRDAVRIARADGSDEIISFDTLAAGSARFAHWLDANDVQPGDRIAFMLEPSLPFY
                     VCLFGAMQSGAISVPLFTLFGPDALRLRIDDCKPTILITNAEKAELARQTPGLRVVVA
                     DDGLLEEIEKYPARYEPRTKADDLAVFQYTSGTTRELPAAVKHTHKAIVTLMFAALYG
                     TGIRPGDEFFCPSSPAWGHGLWHGTLAPLAMGVTTGTFAGRFDAVRLLKALQDFRITN
                     MSAAATHYRMMKNTGQADRFKFCFKKLSFTGEPIDPATLDFIDQYFHVPACSMYGTTE
                     IGVVLVNYPGAADFKVKPGSLGKPIPGLKLQVQRPDGTPTEAGEVGELMLWKKDHWET
                     TKDRAKLDDEGYLYHCGRADDVIISAGWTMSAVEIEHTMLRHGNVIEVGVIGVPDATR
                     GQVAKAFVVSNRAPSDEFVTELQNFTREKLSQ"
     gene            1720..2547
                     /locus_tag="VFX11_11770"
     CDS             1720..2547
                     /locus_tag="VFX11_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX4939346.1"
                     /translation="MTRQTNRATSNDQKLANALKESKAPAISRAAAILRLLGKSDSPL
                     GLQTIARSLNLVPSTCLYVLRALVDEEFVSFDPDTKRYSLEAGVLTLARHWLRRNQFT
                     DLSQPVLNRISQDFDVTVLGVNVVGIEHIIVVGISQAGNNFQISAQVGSRFPALISAT
                     GRCIVAFGNYSEEELQEKFKTLRWDEPPTFSEWKAQINKTRTQGFAVDEGNYISGVTV
                     VAAPVWKSRDKLSHALVAIGISSTLKRTGLPELQDALLKSAQMLSNQLSGDIVLPPR"
     gene            complement(2634..3800)
                     /locus_tag="VFX11_11775"
     CDS             complement(2634..3800)
                     /locus_tag="VFX11_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4939347.1"
                     /translation="MTDKILDCSDLDQHMGKPIAPARMKEPLHNNDFRRWVQAMHYPN
                     LLHYDHDFSAAGRYGRLVAPQSFAIATDDGHGAFPACVGRIPDSHLIFGGDEWWFYGP
                     KIFAGDMITNEKVPFDYAVKETKFAGPTCFQRGDNNYYNQNGDFIAKQRSTTIRYRAD
                     LALEMGSMKDSTEDPEWSPEQLEELEVRKYDYIKMMHALGHGKRYWDDIVVGSELPVR
                     VIGPHSIASFTTEWRAYLFTIWGGTHRPGVDMEALGFTKEMAGHENDPVMEKDNPELT
                     DGAYFGPSRGHLFPQYARKIGMPRGYGYGASMGAWILDYLNGWAGEWGQVVHCNSSYR
                     GPAFTGDCTIMTGTIVDRLVDAEGRNIVRVDCKLTNQLGNVMATAKAEVELPKK"
     gene            complement(3830..>4311)
                     /locus_tag="VFX11_11780"
     CDS             complement(3830..>4311)
                     /locus_tag="VFX11_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEX4939348.1"
                     /translation="VAEKGRGRVLAVQSGKVETLASDLNQPSGVAFSDGSCLVAEEGA
                     GRVVKIVSGGVETVLGGLHKPQGILVRGESLFVVDAGTKELIRYNMTSKIRHTIASLL
                     PVGAPPGVIPKVLGAIGVLSGPMGPFAGIAEGSDGTLYISADAEGSVLELRPETSNH"
BASE COUNT          948 a         1282 c         1138 g          943 t
ORIGIN      
        1 tgctggctta atttctcgcg cgtgaaattc tgcagttctg taacaaattc atcgctgggt
       61 gcgcggttgc tgacgacaaa agccttcgca acctggccgc gcgtcgcgtc gggcaccccg
      121 atcacgccca cttcaatgac attgccatgc ctgagcatgg tgtgctcgat ttccaccgca
      181 ctcatggtcc atccggctga aatgatcacg tcgtcggcac ggccgcaatg atagagatag
      241 ccttcgtcat cgagcttggc gcgatccttg gtggtctccc agtgatcctt tttccatagc
      301 atcagttcgc caacttcgcc ggcctccgtt ggcgtcccat caggccgctg cacttgaagt
      361 ttcaggcctg gaatgggctt tcccaacgaa cctggcttga ccttgaagtc cgcggcgccg
      421 ggatagttga ccagcacaac gccaatttca gtggtgccgt acatactgca ggcgggcaca
      481 tgaaaatact ggtcgatgaa gtccagcgtg gccgggtcga tcggttcacc agtgaaagac
      541 agctttttga agcagaattt gaaacggtcg gcttgcccgg tatttttcat catccggtaa
      601 tgcgtggcag cagctgacat gttggtgatc ctgaagtcct gcagagcctt cagcaggcgc
      661 accgcgtcaa accgcccggc aaatgtgcct gtggtaacgc ccatcgccag aggcgccagt
      721 gtcccatgcc ataatccgtg cccccatgcc ggagaggacg ggcagaaaaa ctcgtcacct
      781 ggacggatac cggtgccata aagcgccgcg aacatcagcg tcacaatcgc cttgtgcgta
      841 tgcttgactg ccgctggcaa ttcccgtgtg gtacccgatg tatactgaaa taccgcaaga
      901 tcatcggctt tggttctggg ttcataccgg gccggatatt tctcaatttc ttccagcaaa
      961 ccgtcatccg ccacaaccac ccgcaggcct ggcgtctgac gcgccagctc cgccttttca
     1021 gcgttggtaa tcaggatcgt cggcttgcaa tcatcgatgc gaaggcgcag agcatccgga
     1081 ccaaacagcg tgaacagcgg cacactgatc gcgccgcttt gcatcgcgcc aaacaggcaa
     1141 acataaaatg gcagcgacgg ctccagcatg aaagcgatgc gatcgcctgg ctgtacgtca
     1201 ttggcatcca gccagtgggc aaaacgtgct gatcccgctg cgagcgtgtc gaagctgatg
     1261 atttcgtcac tgccatcggc acgggctatg cgcacagcat cgcggccgga gccatcggca
     1321 tgacgcgtaa tgcattcgtg ggcaatattg agatgttccc tgtcaccatc aaacagatcc
     1381 cacagcgctg cggaactggc atgcgcctgg gcatccgcat agctggaata ttccgtgagt
     1441 cttgccattc attcacctgg tctttaacca ataaatcgaa cccggccccc tcggccaaat
     1501 acggcaaaac cgggtttggg cttatccctg tatcacccag gaagcgtgcg attcaggacg
     1561 cgttttatgt cgtccgcaaa cgatgacatg aaagattttt gttgtaaata tagaaacaaa
     1621 ttttatatat tgaacacctg gctcggccgc atcgtcagga cagtcgcccg gatgggattg
     1681 gctgtctaaa ggtaagtacg tttattcagg ggtaaaacaa tgactcgtca aaccaaccgc
     1741 gctacatcaa acgaccagaa gcttgccaat gcattgaagg agagcaaggc ccccgccatt
     1801 tcccgcgccg cggcgatcct gcgactgctg ggcaagagcg attctcccct tgggctgcaa
     1861 accatcgcgc ggtcattgaa tctggtgccc agcacctgtc tctacgttct gcgggctttg
     1921 gtggacgaag agttcgtctc ctttgatccc gacaccaaac gttattcgct ggaagccggc
     1981 gtgctcacgc tggcacgaca ttggctcaga cgaaaccagt tcaccgatct ctcccagccg
     2041 gtgctcaatc gcatcagcca ggatttcgac gttaccgtgc ttggcgtcaa cgtcgtggga
     2101 atcgagcaca tcattgttgt cggcatttcc caggccggaa acaatttcca gatcagcgcc
     2161 caagtgggaa gccgttttcc tgcgctgatc agtgcaactg gccgctgcat cgttgcgttc
     2221 ggaaattact cggaggaaga actgcaggaa aaattcaaaa cactccgctg ggatgaacca
     2281 cccacctttt ccgaatggaa agcgcaaatc aacaagacgc gcacacaggg ttttgccgtc
     2341 gatgaaggca actacatttc aggagtgact gttgtcgcgg cgcctgtctg gaagtcccgc
     2401 gacaagctga gtcacgcact ggtagccatc ggcatcagca gcacactgaa gcgaaccgga
     2461 ctgcccgagc tgcaggatgc ccttctcaaa agcgcgcaga tgctgtcgaa ccagctgagt
     2521 ggcgacattg tgctgcctcc gcgctaacgg atcactgccg cgaccgttgt tggtgctaca
     2581 gaccacgccg tagcactgac atcggcagcc gcggcttcgc gtatccagct tccttatttt
     2641 tttggcaatt caacttctgc tttagccgtt gccatgacat tgccaagttg attggttaac
     2701 ttgcagtcca cacgtacgat gttgcggcct tcagcatcca ccagtcggtc aacaatagtg
     2761 cccgtcatga tggtgcagtc gccggtaaac gccgggccac ggtacgatga attgcagtgc
     2821 actacctgcc cccactcacc cgcccaacca ttcagatagt ccagaatcca ggctcccatc
     2881 gacgcgccat agccataacc tcggggcatg ccgatcttgc gcgcatactg cgggaaaagg
     2941 tggccacgcg aagggccgaa gtaggcgccg tccgtcaatt cagggttgtc cttctccatg
     3001 acgggatcat tttcgtgccc cgccatttcc ttcgtgaatc ccagtgcttc catatcgacg
     3061 cctgggcggt gagttccgcc ccagatggta aacagatagg cgcgccactc cgtggtaaaa
     3121 ctggcgatac tgtgaggacc aattacccgc accggcagct cactgcccac aacgatgtca
     3181 tcccaatagc gcttgccgtg gcccagcgcg tgcatcatct tgatatagtc atacttcctg
     3241 acttcgagct cttcgagttg ctccggcgac cactcaggat cttcggtact gtccttcatc
     3301 gatcccatct caagcgcgag atcggcgcga tagcgaatcg tggttgaacg ctgtttggca
     3361 atgaagtcgc cattctggtt gtaatagttg ttgtcgccgc gctggaagca ggtaggtccg
     3421 gcgaatttgg tttccttgac ggcgtaatcg aaaggcacct tctcattggt gatcatgtcc
     3481 ccggcaaaaa tttttgggcc atagaaccac cattcatcac caccaaaaat cagatgggaa
     3541 tcgggaattc gaccaacgca ggcaggaaat gctccatgcc catcgtcggt ggcaatggca
     3601 aacgactgcg gcgccaccag gcgcccgtaa cggcctgctg ctgaaaaatc gtgatcatag
     3661 tgaagcaggt tgggatagtg catcgcctgc acccagcgtc ggaaatcatt gttgtgcagt
     3721 ggctccttca tgcgcgcggg cgcgataggc ttgcccatgt gctggtcgag atcagaacaa
     3781 tccagaatct tgtcggtcat gctttactcc agtcggataa attttgaatt cagtggttgg
     3841 acgtttccgg tcgcagctcc agcacgctgc cttcggcatc ggcagaaata taaagcgtac
     3901 cgtcggagcc ttctgcgatg cctgcaaagg ggcccattgg gccggacaaa accccaattg
     3961 cacccaacac tttcggaatg acgcccggag gcgcgccgac gggcaacagg gacgcgatgg
     4021 tatgacggat tttgctggtc atgttgtacc ggatcagttc cttggtgccg gcatcaacca
     4081 caaacagcga ttctccgcgc accagaatgc cctgcggctt gtgcaatccg cccagtacag
     4141 tctccacgcc gccgctgacg attttgacca cgcgacctgc accttcttcg gcgaccagac
     4201 agctgccatc actgaaggcc acgccggaag gctggttcag atcgctggcc agtgtctcca
     4261 ccttgccgga ctgcaccgca agcacccggc cccttccctt ttccgccacc a
//
LOCUS       DASUEG010000625         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2104280, whole genome shotgun sequence.
ACCESSION   DASUEG010000625 DASUEG010000000
VERSION     DASUEG010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104280"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="serC"
                     /locus_tag="VFX11_11785"
     CDS             complement(<1..740)
                     /gene="serC"
                     /locus_tag="VFX11_11785"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX4939349.1"
                     /translation="MTRAYNFCAGPAALPEAVLKKAQAEMLDYQGRGLSVMEMSHRSD
                     YYIAIAQKAERDLRDLMGIPDHYKVLFLQGGASLQFAQIPLNLLGDKSSADYVNTGEW
                     SKKAIKEAKRYCSVNVVASDEANNFTRVPAFNTWKLDPNAAYLHYTPNETIHGVEYDF
                     IPDVKVPLVADLSSSILSEPLDVNRFGLIYAGAQKNIGPAGITVVIVREDLLGKALPI
                     TPTMLDYKTHSDNDSMYNTPPTYAWYLAG"
     gene            complement(746..>2980)
                     /gene="gyrA"
                     /locus_tag="VFX11_11790"
     CDS             complement(746..>2980)
                     /gene="gyrA"
                     /locus_tag="VFX11_11790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017477315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4939350.1"
                     /translation="ETVDFVPNYDGSEQMPSVMPARIPNLLINGSSGIAVGMATNIPP
                     HNLGEVVDGCLACIDNPDIDIDGLMDFIKGPDLPTYGIINGRSGIIEAYRTGRGRMYM
                     RARAHIESDKNGKESIIVTEIPYQVNKVRLIEKIVELVREKKIEGITDIRDESDSNIR
                     MVIEVRKGDNAEVLLNNLYSQTQMESVFGINMVALEDGQPRLMNLKGIIEAFIRHRRE
                     VVTRRTVYDLRKARERGHVLEGLAVALANIDPVIELIKSSPSRAEAEERLIAKGWQPG
                     NVLAMLERAGSMDACRPEGLDPRFGLKDGLYYLSDAQAKEILDMRLHRLTGLEHDKLI
                     AEYQEKLDLIRELLAILGDSKLLMQVIRDELVAIREQYADERRSEIVVSRMDLTNEDL
                     IPEENVVVTLSHGGYAKTQPIDTYQAQRRGGKGKAAAAIKDEDYIEHLMVASTHDTIL
                     CFTSQGKVYWLKVYQIPIASRTSRGRPIVNLLPLSADEKITAILPLREYESGKFVFMA
                     TSNGTVKKVELEAFSRPRTSGLIAIELEENNRLVGVAITDGSQDIMLFTSEGKAIRFQ
                     EQDVRGMGRTARGVRGVNLPDNVHLVSLIVPQEGHQVLTASENGYGKRTAVEEFPTKG
                     RAGKGMIAMAISERNGNMVGAVQVKDGEEIMLISDQGTLVRTRVDEVSVQGRNTQGVM
                     LIRLSAGEKLVGLERIAELEGAAEADEDFEGDNVGAGPTLDAGGAADGIDQSTEDPHD
                     EQET"
BASE COUNT          520 a          906 c          900 g          654 t
ORIGIN      
        1 cccgccagat accaggcata ggtgggcggc gtgttgtaca tggaatcgtt atcggaatgc
       61 gtcttgtagt ccagcatggt gggcgtgatc ggcagggcct tgcccagcag atcctcgcgc
      121 acgatcacca cggtgatgcc ggccgggccg atatttttct gggcgccggc gtaaatcagg
      181 ccgaaacggt tcacgtccag cggctctgac aagatgctgg acgacaggtc cgccaccagc
      241 ggcaccttca cgtcaggaat gaaatcgtat tccacgccgt ggatggtttc gttgggcgtg
      301 taatgcaggt aggccgcatt cgggtccagc ttccaggtgt tgaaggccgg cacccgggtg
      361 aaattgttcg cttcgtcaga cgccacaaca ttgacgctgc agtaacgctt cgcttccttg
      421 atcgccttct tcgaccactc gccggtattg acgtaatcgg cagagctttt gtcacccagc
      481 agattgagcg ggatctgggc gaactggagg ctggcacccc cctgcaggaa cagcaccttg
      541 taatggtcgg gaatacccat caggtcgcgc aggtcgcgct cggctttttg cgcaatggcg
      601 atgtaatagt ccgagcgatg gctcatttcc atcacggaca ggccacggcc ctggtaatcg
      661 agcatttctg cctgggcctt cttcaggacg gcctccggca gcgccgccgg acccgcacaa
      721 aagttatacg cacgcgtcat ggttttcagg tttcctgttc gtcatgagga tcttcggttg
      781 attgatcgat gccatctgca gcaccaccgg cgtccagtgt agggccggcc ccgacattgt
      841 caccctcgaa atcttcatcc gcttctgcag cgccttcaag ttcggcaatc cgctccagcc
      901 ccaccagttt ttccccggca gacaggcgga tcagcatcac gccctgagtg ttgcggccct
      961 gcaccgagac ttcatccacc cgggtgcgta ccagggtgcc ctgatcggaa atcagcatga
     1021 tttcctcgcc gtccttcacc tggaccgcgc cgaccatatt gccgttccgc tcgctgatgg
     1081 ccatggcaat catgcccttg cccgcgcggc ccttggtggg gaactcctcc acggcagtac
     1141 gcttgccata gccgttctcg ctggcagtca aaacctgatg gccctcctgc ggcacgatca
     1201 gggataccag atgcacattg tccggcaggt tcacgccgcg cacgccacgg gcggtccggc
     1261 ccatgccacg cacatcctgc tcctggaagc ggatggcctt gccttcggat gtgaagagca
     1321 tgatgtcctg gctgccgtcg gtaatggcga cgcccaccag acggttgttc tcttccagct
     1381 cgatggcaat caggccgctg gtgcgcggac gggaaaacgc ttccagttcc actttcttca
     1441 cggtgccgtt gctggtggcc atgaacacga acttgccgga ttcatattcc cgcagcggca
     1501 ggatggcggt gattttctcg tcggcggaca gcggcagcag gttgacgatc ggacggccac
     1561 gggaagtgcg gctggcgatc ggaatctggt agaccttcag ccagtacacc ttgccctgac
     1621 tggtgaagca gagaatggtg tcgtgggtgc tggccaccat caggtgctcg atgtagtcct
     1681 cgtccttgat cgcggcggcc gccttgccct tgccaccccg gcgctgggcc tggtaagtgt
     1741 cgatgggctg ggtcttggcg tagccgccgt gggacagggt caccaccacg ttttcttcgg
     1801 ggatcaggtc ttcgttggtc aggtccatgc gcgaaacgac gatttcgctg cggcgttcat
     1861 cggcgtattg ctcgcggatc gcaaccagct cgtcccggat cacctgcatc agcagcttgg
     1921 aatcgcccag aatcgccagc aattcacgga tcaggtcgag tttttcctgg tattcggcga
     1981 tcagcttgtc atgctccagc ccggtcaggc ggtggaggcg catgtcgaga atttccttcg
     2041 cctgggcgtc ggacaggtag tacaggccat ctttcagacc gaaacggggg tcgagccctt
     2101 ccgggcggca ggcatccatg ctgccggcgc gctcgagcat ggccagcacg ttgcccggct
     2161 gccagccttt tgcgatcagg cgctcttccg cctcggcgcg gctgggggag ctcttgatca
     2221 gttcgatcac cggatcgatg ttggccagcg ctaccgccag accttccagt acgtggccgc
     2281 gctcgcgggc cttgcgcagg tcatacacgg tacggcgggt caccacttca cggcggtgac
     2341 ggatgaacgc ctcgatgatg cccttcaggt tcatcagacg tggctggccg tcttccagcg
     2401 ccaccatgtt gataccaaac acgctttcca tctgggtctg cgagtacaga ttgttcagca
     2461 gcacctcggc gttgtcgccc ttgcggacct cgatcaccat gcggatgttg ctgtcggatt
     2521 catcgcggat gtcggtgatg ccttcgattt tcttttcccg gaccagctcg acgattttct
     2581 cgatcaggcg aaccttgttc acctgatagg gaatctcggt aacgatgatg gattccttgc
     2641 cgttcttgtc ggactcgatg tgcgcgcggg cgcgcatgta catgcggcca cggccggtgc
     2701 ggtaagcttc gatgatgccc gagcggccat tgatgatgcc gtaagtgggc agatcggggc
     2761 ccttgatgaa atccatcagg ccgtcgatgt cgatatcggg gttgtcgatg caggccaggc
     2821 agccatccac cacttcgccc aggttgtggg gcggaatgtt ggtggccata cccacggcaa
     2881 taccggagga accgttgatc agcaggttgg gaatccgcgc cggcatcacc gacggcatct
     2941 gctcggagcc gtcatagttg ggcacgaaat cgacggtttc
//
LOCUS       DASUEG010000626         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_25276, whole genome shotgun sequence.
ACCESSION   DASUEG010000626 DASUEG010000000
VERSION     DASUEG010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25276"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFX11_11795"
     CDS             <1..254
                     /locus_tag="VFX11_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4939351.1"
                     /translation="KACIGSGIELPACAGYLTASPKTVFWLPELSMGLIPGAGGCVSI
                     SRRIGRRRTAYLVLMNKKIDAQKALAWGLIDGIVPEDAD"
     gene            complement(301..1575)
                     /locus_tag="VFX11_11800"
     CDS             complement(301..1575)
                     /locus_tag="VFX11_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4939352.1"
                     /translation="MDALGGALIPGLNDHHIHFLSFAASLASIDCSPAATPNREELAA
                     LLQQQTASNAWLRGFGYHESIAGDIDCHWLDRHCPNRPTRIQHRSGRLWIFNSAGLAM
                     LQELLLLQSTPPPLPAESFTSGRFYDCDQALAALLGRHLPPVKSASEKLASYGVTGFT
                     DMTPSNDQDTFALFHRLRQERSILQNIQLARYTAFEPGVTGAAYSGPVKIHLHENRLP
                     DLQELVERILASHTAGIAVAIHCVTEIELLFSLAALEEAGSMTGDRIEHAAVAPEHTL
                     ERMRQLGVTVVTQPHFILEKGDAYLRDVESSDHEALYRCHTLLNQNIPLAGSSDAPFG
                     SADPWTAMHAAVSRRTASGIVLGHNEALNPEQALALFLGSLDAPGQIRQLQPGVAADL
                     CLLKHPWAVARNRLTSDDVHFVMSRGQPIFSR"
     gene            1874..2506
                     /locus_tag="VFX11_11805"
     CDS             1874..2506
                     /locus_tag="VFX11_11805"
                     /inference="COORDINATES: protein motif:HMM:NF039921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939353.1"
                     /translation="MEKTLLNKHGQKMGRKGLVMRKRIMEKALELIRDSSYKDLTVAD
                     VAYEADTSTSTFYVYFTDIEDVLFACVEEADQDLGRIMDVLNREWTMDNMEAMVSEFV
                     DSYMNVWEKHSVELRIRNQEADQGNLRFLQFRLDSTRDIIEGLEKKVAQAQPHAKNPL
                     SLVLVLFTAMERLAGANFRAMTGPTRITRKKLNDAIKELWMMLLMKGPCE"
BASE COUNT          570 a          701 c          846 g          609 t
ORIGIN      
        1 ggaaggcctg cattggcagt ggtatcgagc tgcctgcctg cgccggttac ctgacggctt
       61 cacccaaaac cgtgttctgg ctgcccgaac tgtccatggg attgattccc ggtgccggtg
      121 gctgcgtcag tatttcgcgg cggattgggc ggcggcgaac ggcctatctg gttttgatga
      181 acaaaaagat tgacgctcaa aaagcgctgg cgtggggcct gattgatgga atcgttccgg
      241 aagacgccga ctgactgcac ggtaatcgct gttacccgac atccgctcgg ctgacgtttg
      301 tcatcgactg aatatcggtt gtcccctgct catgacgaag tgaacgtcgt cgctggtcag
      361 gcgatttctg gcaactgccc acggatgttt gagcaggcac aggtcagcgg ccacgccggg
      421 ttgaagttgg cgtatctggc cgggtgcatc cagtgagccc agaaacaatg ccagcgcctg
      481 ttccggattc aatgcttcgt tgtggccgag cactattccg gatgccgtgc ggcgcgaaac
      541 ggccgcatgc atggcggtcc agggatcggc tgatccgaaa ggcgcgtcag agcttcccgc
      601 caggggaata ttctgattca ggagtgtgtg gcaacgataa agcgcttcgt gatcgctgga
      661 ttccacgtcg cgtagatagg cgtcaccctt ttccagtatg aagtgaggtt gggttaccac
      721 cgtcacaccc agttgccgca tgcgttcgag tgtgtgctcc ggtgcgacag ctgcatgttc
      781 gatgcgatcg cctgtcatgg aaccggcttc ttccagtgcc gcgaggctga acagaagctc
      841 gatttcggtt acgcaatgga tcgcgactgc aatgcccgcg gtgtggctcg caagaattcg
      901 ctctaccagt tcctgcaagt caggcaggcg attctcgtgt aaatggatct tcaccgggcc
      961 ggaataggct gcgccagtga ccccgggttc aaatgcggtg tatcgggcca gctgaatatt
     1021 ctgcaggatc gaacgttcct gtcgcagccg gtggaacaat gcgaacgtgt cctgatcatt
     1081 ggaaggcgtc atgtcggtaa aaccggtaac gccataggac gccagctttt cactggcgga
     1141 ctttaccggc ggcaggtgac ggcccagcag ggctgccaac gcttgatcgc aatcgtaaaa
     1201 ccggccggac gtgaagctct ctgccggcaa gggtggcgga gttgattgca gcagaagtaa
     1261 ttcctggagc attgcaagcc ctgccgaatt gaaaatccag agcctgccgg agcggtgctg
     1321 aattcgggtg gggcggttag ggcagtggcg gtcgagccaa tggcagtcaa tgtctcccgc
     1381 aatgctttcg tggtagccga atccccttaa ccacgcattg cttgcggttt gttgttgcaa
     1441 aagtgcggcc agttcctcgc ggttgggtgt ggcggcaggg ctgcaatcta tggaggccag
     1501 agacgcagca aaactgagga aatgaatatg atgatcgttg aggccgggga tcagtgcgcc
     1561 gccgagtgca tccacaacgg tttcgttggg gcggcaggaa aggcagccga tttcgctgat
     1621 gatgcctcgg tcgatgcgca ggtcgttcag acctgcctga tagatttcgg cgttcctgat
     1681 caacatttca cggtgctgtc attggttggg attccccgtt gagtattcgc atcccgtaac
     1741 cgggtcgcgt aatcgtgggg aacgctcggg cgtgtttgca taacctacca caaaagataa
     1801 taatcgtcag ttattgcatc gccggttggc aaatccggca aggttccgaa aaggcatttg
     1861 tggagacatc agggtggaaa agacgttgct gaacaagcac gggcagaaga tgggtcgcaa
     1921 ggggctggtc atgcgcaagc gcataatgga aaaggcgctt gagctcatca gggattcttc
     1981 ctacaaggat ctcactgtcg ccgatgtcgc ctatgaggcc gacacttcca cttccacttt
     2041 ttatgtgtat ttcaccgata tcgaggatgt gctgttcgcc tgcgttgaag aggcggatca
     2101 ggatctcggc cgcatcatgg atgtgctgaa ccgtgaatgg accatggaca acatggaggc
     2161 gatggtgtcc gagtttgttg attcctatat gaatgtctgg gaaaagcatt cggtggagct
     2221 gcgcattcgc aatcaggaag ctgaccaggg caatctccgc tttctgcagt tccggctgga
     2281 ttccacccgg gacattatcg agggcctgga aaagaaagtc gcgcaggcgc agccccatgc
     2341 caaaaatccg ctgtcgttgg tgctggtgct gttcacggca atggaacggc tggcgggagc
     2401 caattttcgc gccatgacgg ggcctacgcg catcacccgg aaaaagctga acgatgcgat
     2461 caaggagcta tggatgatgt tgttgatgaa gggtccctgc gaatagatcc agctcttcca
     2521 gtacgggcgt gttgtgctgg ccgggttctg cgcatgcggc tgtcggaatg cgatgtcctc
     2581 ctggaaaaaa cagatgcagt tgattgccca gccgatgcca ttgccggcag gattccagca
     2641 cattctcgcg tgccagattc agctcgctgt gcaggcaggc agacactgtg cctcgcagcg
     2701 atagcgagac caatgcattg ccaccc
//
LOCUS       DASUEG010000627         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1797575, whole genome shotgun sequence.
ACCESSION   DASUEG010000627 DASUEG010000000
VERSION     DASUEG010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797575"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFX11_11810"
     CDS             complement(<1..1023)
                     /locus_tag="VFX11_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4939354.1"
                     /translation="MKSNTRKTLLMAMLPFLLLDVSGCGGGSRKDAPDAAPSPGCDGS
                     CANASTFLSVADVERILAQGITEAQARGVAATFAVSDRVGNVLAVYRMTGANPAGVEI
                     STHTGTSGGLEGVVLPAAADGLAAIAKALTGAYLSSEGNAFSTRTAGQIVQDHFNPGE
                     NFQPAGPLFGVQFSQLACSDLTTSLADGSFGPKQSPLGLAADPGGFPLYKNGTVVGGV
                     GVIADGDYSVDLNILDRDSDLDELIAYAAAFNYAAPVDRRANRITADGKTLRFSDVDS
                     GDLNTRPDNAPAFGSIGGGVGALVAVSGYNAGAISAGTAFGQPASGIRADGGSFADLD
                     AFILVDG"
     gene            complement(1064..2764)
                     /locus_tag="VFX11_11815"
     CDS             complement(1064..2764)
                     /locus_tag="VFX11_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX4939355.1"
                     /translation="MRCLIRTIAAQQETLEYRDAEYVVSEISLGAASDCTVHLEGRGV
                     LPRHALITLRRDGRVQIKADGGAALLINEKPQRSAVLAKGDTVRIGQNLFTVIVAPFG
                     FDLALSLAIEGAAQEADTLEFELGPMSLSEAGWSRRRWAWLGMLVVLSVGLILPLSWS
                     LWPGEPEARPAHWPVTDNAWSSGPLHTAHRIPGLIDDCGACHEAPFVQVQDTACKNCH
                     EVPDHVAHPEKAPAFAGLDCAGCHREHNEPQTLVRMDEALCVDCHGDIAQHWPEKSAL
                     QNVADFDSHPEFKLSLLQQQVGTGDAANGVEKMEWKTQRIPQNDPTLAEHSNLKFSHA
                     QHLNADGIDGPDGKAVMACADCHQPQQNGMLMQPIQMEQHCAGCHSLAFEATDPERQV
                     PHGSPQDVLRSLEEYYARLYAIQGGPVESVHELSRPAQRPGKPPKSFYAAMKAWADER
                     ALEAATTLMEESACHSCHEVTVHADKEDASRWSVLPVKLTEVWMPKSRFDHGAHLQIE
                     CGDCHQAAKSEKPTDVLLPDLKSCKQCHGGEGSNMMVSTGCVGCHGYHQTGIAKGQSQ
                     "
     gene            complement(2769..>3020)
                     /locus_tag="VFX11_11820"
     CDS             complement(2769..>3020)
                     /locus_tag="VFX11_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939356.1"
                     /translation="NRNQDRVIRALAERLARSPGGKEAVLLQELLWLFARRKTLLQQV
                     RADVRMQGWLQVWLLVHVPLSLALMVALALHIFSVFYYW"
BASE COUNT          601 a         1020 c          823 g          576 t
ORIGIN      
        1 gccgtccacc agaataaacg catccagatc ggcaaaactg ccgccatcgg cgcgaatacc
       61 ggaagccggc tggccaaatg cggtgcccgc actgatcgca ccggcgttat atccacttac
      121 cgccaccagc gcgccgacac cgccaccgat gctgccaaac gcaggcgcgt tgtccgggcg
      181 ggtattcaga tcacctgaat cgacatcgct gaagcgcagt gttttgccgt ccgccgtgat
      241 gcgattggcg cgacgatcca ccggcgcggc gtaattgaaa gccgcagcat aggcgatcag
      301 ttcatccaga tccgaatccc gatccagaat attcaggtca acggaatagt cgccgtcggc
      361 aataacgcca acgccgccca caacggtgcc atttttatat aaaggaaacc ctccgggatc
      421 agccgccaga cccagcggcg actgcttggg cccgaacgaa ccgtcggcca gcgacgtggt
      481 gagatcactg cacgccagct gactgaactg cacaccaaac agcggaccgg ccggctggaa
      541 attttcgccg ggattgaaat gatcctgcac gatctgtcct gccgtacggg tgctgaatgc
      601 attgccttca ctggacaggt acgcccctgt caaggccttc gcgatggccg ccagcccatc
      661 ggccgccgcc ggcagcacca cgccttccaa cccgccgctg gtgccagtgt gagtggaaat
      721 ttcaacgcca gcgggattcg cccccgtcat ccggtacacc gccagcacat tgccgacccg
      781 atcgctcact gcaaacgtcg ccgccacgcc gcgcgcctgc gcttcggtaa taccctgcgc
      841 cagaatgcgt tccacatccg ctaccgacag aaatgtgctg gcgttggcgc aactgccatc
      901 gcaaccgggt gaaggcgccg catccggtgc atccttgcgg gaaccaccgc cgcagccgct
      961 tacatccagc agcagaaaag gcagcatggc cattaaaagc gttttgcgtg tgttcgattt
     1021 cattttgccg tttcccgatt cggtccagcg cacctgtgcg cactcactgc gactgccctt
     1081 ttgcgatacc tgtctggtga taaccgtggc aacctacaca accggtggac accatcatgt
     1141 tgctgccttc gccaccatga cactgcttgc agcttttcaa atccggcagc agcacatccg
     1201 tgggcttttc ggatttggcc gcctgatggc aatcgccgca ttcgatctgc aaatgggcgc
     1261 catgatcgaa gcgggatttt ggcatccaga cttccgtcag cttcaccggc agcacgctcc
     1321 agcgcgacgc atcttctttg tcagcgtgca ccgtcacctc gtggcaactg tggcaggcac
     1381 tttcttccat cagcgtagtg gcagcctcca gcgcgcgctc gtcggcccag gctttcatcg
     1441 ccgcataaaa ggatttcggt ggcttgcccg ggcgttgcgc aggacggctc agctcatgca
     1501 ccgattccac cggcccgccc tgaatcgcat aaagacgcgc gtaatattct tccagcgaac
     1561 gcaacacgtc ctgcggcgaa ccgtggggca cctgtcgttc gggatcggtg gcctcgaacg
     1621 ccagcgaatg acagccagcg caatgctgtt ccatctggat cggctgcatc agcatgccat
     1681 tctgctgcgg ctggtggcag tcggcacagg ccatcaccgc tttgccatcc ggcccgtcga
     1741 taccgtcggc attcaagtgc tgggcgtggg aaaatttcag gttggaatgt tccgccagag
     1801 tgggatcatt ctgcgggatg cgctgggttt tccactccat tttttcaaca ccgtttgccg
     1861 catcgccagt gccaacctgc tgttgcaaca ggctcaattt aaactccggg tgggaatcaa
     1921 aatccgccac attctgcagt gcagattttt ccggccagtg ctgcgcgata tcgccgtgac
     1981 aatccacgca cagagcctcg tccatccgca ccagggtttg cggctcattg tgctcgcggt
     2041 ggcagccggc gcaatccagc ccggcaaagg ccggcgcttt ttccggatgc gccacgtgat
     2101 caggaacctc atggcaattt ttgcaggctg tgtcctgcac ctgcacgaag ggcgcttcat
     2161 ggcaggcgcc gcaatcatcg atcaaaccgg ggatgcgatg agcggtatgc agtggcccac
     2221 tgctccaggc gttgtcagtt accggccagt gcgccggtcg cgcctctggt tcgcctggcc
     2281 acaggctcca gctcagcggc agtatcagtc caacagaaag caccaccagc atgcccagcc
     2341 aggcccagcg acgccggctc caacccgctt cgctcaaact catcgggccc agctcgaatt
     2401 ccagcgtgtc tgcttcctgc gccgcgcctt cgatggccag cgacagcgcc agatcaaacc
     2461 cgaacggcgc aacaatcacc gtaaacaggt tctggccgat gcgcacggtg tcgcccttcg
     2521 ccagcaccgc actgcgttgc ggtttttcat tgatcagcaa cgcggcaccg ccatcagcct
     2581 tgatctgcac gcgaccgtcg cgccgcagcg tgatcagcgc gtggcgcggc agcacaccgc
     2641 gcccttccag atgcacggtg caatcgctgg cggcgcccag gctgatctcg ctgacgacat
     2701 attccgcgtc gcggtattcc agcgtttctt gttgggcggc gatggttcgg atcagacagc
     2761 gcatggcatc accagtaata gaaaacgctg aaaatgtgca atgccagcgc caccatcaac
     2821 gccagcgaca acggcacatg caccagcagc cacacttgca gccagccctg catacgcaca
     2881 tcggcgcgca cctgctgcaa cagcgttttg cgccgcgcga acagccacag caattcctgc
     2941 agcaacaccg cctctttgcc acccgggctg cgcgccagcc gctccgccag cgcgcgaatc
     3001 acccgatcct gattgcgatt
//
LOCUS       DASUEG010000628         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1951327, whole genome shotgun sequence.
ACCESSION   DASUEG010000628 DASUEG010000000
VERSION     DASUEG010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951327"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="ubiG"
                     /locus_tag="VFX11_11825"
     CDS             <1..794
                     /gene="ubiG"
                     /locus_tag="VFX11_11825"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX4939357.1"
                     /translation="EMGSTQTADNSLYDTLSDSWWDERSLLNLLKTTVNPWRVPYFAR
                     LLAQHADIDSPLNLLDVGCGGGLLAEEFASLNCHVTGIDPSEQSLVTARAHAQQQGLD
                     IAYHCGFGDALPFPEASFDVVSCCDVLEHIEDWQATIAEISRVLKPGGLFLFDTINRT
                     SYSWLVNILIAQELPLTRFMPRNMHVWHMFITPAELRAGLRRHGLHQRDLVGASPKSS
                     PLHALKAIRGFKRGHMTAEEVGAVASMRPSRNVRGSYMGYAVKPR"
     gene            850..>2408
                     /locus_tag="VFX11_11830"
     CDS             850..>2408
                     /locus_tag="VFX11_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX4939358.1"
                     /translation="MGSFKITRRTVLGASAAAAAGLVSGSSSADSGRHRQPTVAAVPL
                     PLLFHSPTLQPYVDELPVPPQRRLGGTVVIGEALHQFHRDLAPARSWGYAGATHMGPT
                     FIARRGENIPTVFDNQLQDHIFAKDIDLSQHGTCAIDLLVPPVSVHLHGAPNAPEHDG
                     NAMDLFRPGTSRVYEFNNDIQACTLWLHDHAMGNTRLNVYGGLATQYWVRDEWDTGAA
                     DNPLGLPTGDYEIPLTVQDKMFWDDGMPRFYATPTVPRNWWQGGFCGDVMCVNGKVWP
                     NLNVDRGLYRFRLLNGASLNTYTFYFSNRMNFHVIGTDGGLLDAPVPTHQLTVGVGER
                     FDILADFSGLVPGTQLQLMNDEQIVWAGQMLGSKMIPNIMQFTVQDKTGFQGPVPATL
                     RGGIGQPEKLPALAQPAFVRHSTLNWLLDTRIPFSDMNINNMRYEDEPVEMPVQGRIE
                     QWNFINNSPWDHTMHLHLVQFRIIGRQDYWPLPYLFFNPPPPQKGKRWAPPADRFKRR
                     RPMDRPLPVEDG"
BASE COUNT          474 a          725 c          727 g          482 t
ORIGIN      
        1 gggaaatggg gagcacgcaa acggcggaca acagcctgta tgacacgctg tcggacagct
       61 ggtgggatga acgcagcctg ctcaatctgc tgaaaaccac ggtgaatccc tggcgggtac
      121 cttacttcgc ccgcctgctg gcgcaacatg ccgacatcga ttcacccctc aatctgctcg
      181 atgtgggctg tggcggcggt ctgctggcgg aagagtttgc cagcctgaat tgccatgtga
      241 ccggcatcga tccctcggaa caatcgctgg tcaccgcacg cgcgcatgcg cagcagcaag
      301 ggctggacat cgcctatcac tgcggcttcg gtgacgcgct gccgttcccc gaggcgtctt
      361 tcgatgtggt cagctgctgc gatgtgctgg aacacattga ggactggcag gccaccattg
      421 cggagatcag ccgtgtgctc aaacccggcg ggctgtttct gttcgacacg atcaatcgca
      481 cgtcttacag ctggctggtc aatatcctga ttgcccagga acttccgctg acccgtttca
      541 tgcctcgcaa catgcatgtg tggcacatgt tcataacacc cgccgagctt cgcgccggcc
      601 tgcgcaggca cggtcttcac cagcgcgatc tggtgggcgc ttcacccaag agttcgccgc
      661 tgcatgcact caaagccatt cgcggattca agcgcggcca catgacggca gaagaagtcg
      721 gcgccgtcgc gtccatgcgc ccttcccgca acgtgcgggg cagctacatg gggtacgccg
      781 taaaaccgcg ttgacgagta ctgtcgcccg ctggaagtcg ctccataaca ataagaaagg
      841 agatccacca tggggtcttt caaaattaca cggcggacag tgctgggtgc gagtgctgca
      901 gccgctgcag gactggtgag cggcagcagt tcggccgaca gtggccggca ccggcaaccc
      961 accgtggcgg cagtgccgct gccgctactg ttccactcgc ccacgctgca accttatgtg
     1021 gatgaattac cggtaccacc gcagcgacgg ctgggtggca ctgtggtgat cggcgaggcg
     1081 ttgcaccagt ttcatcgtga tctggcgcct gcgcggtcct ggggttatgc gggtgcaacc
     1141 cacatgggcc caaccttcat cgcgcggcgc ggcgaaaata ttcccaccgt tttcgacaac
     1201 caactgcagg atcatatttt cgcgaaggat attgatctgt cccagcacgg aacctgcgcg
     1261 atcgatctgc tggtgccgcc ggtgtcggtg catttgcatg gcgcacccaa tgcaccggag
     1321 cacgacggca atgccatgga cctgttccgc cccggcacca gccgcgtgta tgaattcaac
     1381 aatgatattc aggcctgcac gctctggctg cacgaccatg ccatgggcaa cacccggttg
     1441 aatgtgtacg gcggcctggc cacgcaatac tgggtgcggg atgaatggga caccggtgcg
     1501 gccgacaatc cgctgggatt gcctactggc gattatgaaa ttccgctcac ggttcaggac
     1561 aagatgttct gggacgacgg catgccgcgc ttctatgcaa cgcccaccgt gccgcgcaac
     1621 tggtggcagg gcggcttttg cggcgacgtc atgtgcgtga atggcaaggt gtggccgaac
     1681 ctgaacgtgg accgcgggtt gtaccggttt cgcctgctga acggagcgtc gctcaacacc
     1741 tacacgtttt atttttccaa ccgcatgaac tttcatgtga tcggtaccga tggcggcctg
     1801 ctggatgcgc cggtgccaac ccatcagctt accgtgggcg tgggggagcg tttcgatatt
     1861 ctggcggatt tctccggcct ggtaccgggc acacaactcc agctgatgaa tgatgagcag
     1921 attgtctggg caggccagat gctgggttcg aagatgattc ccaacatcat gcaattcacg
     1981 gtgcaggaca aaaccggatt ccagggtccg gtgcccgcca cgttgcgcgg cggtattggc
     2041 cagccggaaa aactgcccgc actggcgcag ccggcgttcg tacgtcattc cacgctgaac
     2101 tggctgctgg atacgcgcat tccgttctcg gacatgaaca tcaataacat gcgctacgag
     2161 gacgagccgg tggagatgcc ggtgcagggc cggatcgagc agtggaattt catcaacaat
     2221 tcaccctggg accacacgat gcatttgcat ctggtgcagt tccgcatcat tggccggcag
     2281 gattactggc cattgccgta cctgtttttc aatccgcctc cgccccaaaa gggcaaacgc
     2341 tgggcgcctc cggccgaccg gttcaaacgc agacgcccca tggatcggcc gctgccggtg
     2401 gaagatgg
//
LOCUS       DASUEG010000629         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_22045, whole genome shotgun sequence.
ACCESSION   DASUEG010000629 DASUEG010000000
VERSION     DASUEG010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22045"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFX11_11835"
     CDS             complement(<1..389)
                     /locus_tag="VFX11_11835"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4939359.1"
                     /translation="MSAIPSTAESIAKSVRIPPRPEALVLFRQEMANANPNFNKLAAA
                     VSSDVSLSGAVLQIINSPAFGARSKVTSINHACNMLGLVRVEKVVQAVALRGALSKGV
                     SLDRFWDSANEIANLSRILAQKLSGIPA"
     gene            complement(526..1644)
                     /gene="hemW"
                     /locus_tag="VFX11_11840"
                     /pseudo
     CDS             complement(526..1644)
                     /gene="hemW"
                     /locus_tag="VFX11_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010429620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(1661..2269)
                     /locus_tag="VFX11_11845"
     CDS             complement(1661..2269)
                     /locus_tag="VFX11_11845"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018861194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX4939360.1"
                     /translation="MFSANEIVVATGNKGKLKEIGEVLAPLGIRVRGQQEFAIPEIEE
                     TGLTFVENAILKARNTCAHTGKPALADDSGIEVDALDGAPGIYSARYAGAGATDAANV
                     EKLLAALKGIPEAERTARFQCVMVYLRHANDPVPLICQGTWEGRITLQPQGDNGFGYD
                     PVFWVPQHGCTAAQLASAVKNSLSHRGQALQKLLAALQARHG"
     gene            complement(2346..2792)
                     /locus_tag="VFX11_11850"
     CDS             complement(2346..2792)
                     /locus_tag="VFX11_11850"
                     /inference="COORDINATES: protein motif:HMM:NF025822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HEX4939361.1"
                     /translation="MKTRTALLSALLLLLLSTFVRAEQFVQDDQYIVHYSAFNSTLLT
                     PEVAKAYDLMRSRQRAVMNVTVQRKMPDGSSKAVTSQLEGYTGALGGSERPLDFKMVT
                     EGSAIYYLAEFLIGNGEKLNFDIKVKPTPEHKPIKVEFSQNFFTEE"
     gene            complement(2798..>3046)
                     /locus_tag="VFX11_11855"
     CDS             complement(2798..>3046)
                     /locus_tag="VFX11_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX4939362.1"
                     /translation="YWKCRLYLMLKGKMPVSKVLPYTWYNTPNIHLCTFQDFEALCRR
                     KHLKILNRTVVDQNYRDSFLMRLLPNLFGETAVYHVTR"
BASE COUNT          609 a          861 c          899 g          677 t
ORIGIN      
        1 gcgggaatac cgctcagctt ctgcgccagg atgcgggaca ggttggcgat ttcattggcg
       61 ctatcccaga accggtccag cgacacgcct ttggacaggg cgccgcgcaa tgccaccgcc
      121 tgcaccacct tttccacgcg caccagtccc agcatgttgc aggcatggtt gatcgaggtg
      181 accttgctgc gtgctccgaa cgcgggagag ttgatgatct gcagcaccgc gcccgacagg
      241 ctgacatctg acgatacggc cgccgccagc ttgttgaaat tgggattggc gttggccatt
      301 tcctggcgaa agaggaccag cgcttccgga cggggtggaa tgcgcacgga tttggcaatg
      361 gattcggcgg tgctggggat ggcggacatt gaactgcgaa ctaccactca atggatacga
      421 caatttgagt atagaagaca ggattgatag acaagggcga atgtaagaag aatcgggtta
      481 tatgctgcgg gggatttata tccatcacga ccggcctgca gggcgtcaga acagttgcag
      541 cagttcgttc agaaacagtt gcccctgtcg cgtggtttgc cagtgggtgg tggtttccgt
      601 caacaagcct tttgccttcg cctgtgcaat ggatggagtc acaacatcca gtgccaaacc
      661 ggtacgctcg acaaacagcg ccttcgccac cggctggttc aggcggaacg cattcatgaa
      721 aaattcgaat ggaagttcgg cgtcttcaat ctgtgtttgc tgcgcagtga acggcttgtc
      781 agccgccaga taatctttcg gcagccgggt tttgttgtag cgggtgatgc cgccgttgcg
      841 cagatcggtg atcttgccgt gtgcgccggc accgatcgcc agataatcgc cgaacagcca
      901 gtaattcaga ttgtgcgcgc attcgaatcc gggtttggcg taggcactga tttcgtactg
      961 cgcgaaaccc tgcgccgcga tgaactgctt gccggcctga tcgatttccc acagatgctc
     1021 gtcgtcaggc agggccggtg gtctggaata aaacacggtg ttgggctcga tggtgagctg
     1081 gtaccacgac aggtgcgtgg gttgcaggtc caccgcctgc tgcagatccg ccagtgcgtc
     1141 ctgcagtgtc tgctgtggca ggccgtgcat cagatcgata ttgagttttt caaagcccac
     1201 ttcgcgtgct gcaccaatgg cgcgaatcgc ctcgtcacgg tcatgcacgc ggccgagggc
     1261 ctgcagctgc tgcgaattga aactctgcac gccgacagac aagcgattga tgcccgccgt
     1321 acgaaagccc ttgaacttca ggaattcgaa cgtgcccgga ttggcttcca gcgtgatttc
     1381 cgcatcgcgc gcaagcggtg tgatggcgtg aatgtcctgc agcagccggg cgtagctgtc
     1441 cgccgcaaac aggctggggg tgcccccgcc gataaaaata ctctggatct ggcggccctg
     1501 tgccagtgct gcatcctgcc gaagatcggt gagcagtgcg ttcaggtact ccgcttccgg
     1561 aatcaccttc gcttcgtgac tgttgaaatc acaataggga cacttgcgga tgcaccaggg
     1621 aatatgaatg tagagtgcga gaggaatgct ccggctgtcg tcagccatga cgcgcctgca
     1681 acgcggccag cagtttctgc agtgcctgcc cgcgatggct gaggctgttt ttcaccgccg
     1741 atgccagttg cgccgccgtg cagccatgct gcggtaccca gaacaccgga tcgtagccaa
     1801 aaccgttgtc gccctgcggc tgcaatgtaa tgcgaccttc ccaggtgccc tggcagatca
     1861 gcggcaccgg atcgttggcg tgacgcagat acaccatcac gcactggaag cgcgcggtgc
     1921 gttcggcttc tggtatgccc ttcaaggcag ccagtaattt ttccacgttg gcggcatcag
     1981 tggcacccgc accggcatag cgcgcactgt aaatccccgg cgcgccgtcg agcgcatcca
     2041 cttcaattcc ggaatcgtcg gccagcgccg gcttgccggt gtgggcacag gtgttgcggg
     2101 ctttcaggat cgcgttttcc acgaaggtaa ggccggtttc ctcgatctcg gggatcgcga
     2161 attcctgctg cccgcgcacg cgaatgccca gcggcgccag cacctcaccg atttccttca
     2221 gcttgccctt gttgccggtg gcgaccacga tctcgtttgc ggaaaacatc acactacctt
     2281 tgctgctggt tcaatccggt tgccaccgtg cgcctgagtc cgccgcgcct attcctcggc
     2341 gaagctcatt cctcggtgaa aaagttctgg ctgaattcca ccttgatggg cttgtgttct
     2401 ggggtgggtt tcaccttgat gtcgaaattc agtttttcgc cgttaccgat cagaaattcc
     2461 gccaggtaat agattgcgga gccctcggtc accatcttga aatccagtgg ccgctcgctg
     2521 ccacccagcg cgccggtgta accttccagt tgtgacgtta ccgccttgct ggaaccgtcc
     2581 ggcatcttgc gctgtacagt gacattcatc accgcgcgct gacggctgcg catcaggtca
     2641 taggccttgg ccacttccgg tgtcagcagg gtggaattga aggcgctgta atggacgatg
     2701 tactgatcgt cctgcacgaa ctgctcggcg cggacgaaag tggacaacag taacagcaga
     2761 agagcggaca gcagagcggt tctggttttc atggttctca cctcgtgacg tggtagaccg
     2821 ctgtttcgcc gaacagatta ggcaaaagcc gcatcaggaa actgtcgcgg tagttctgat
     2881 ccactacggt acgattgaga attttaagat gcttgcgacg acataacgct tcaaaatcct
     2941 ggaacgtaca caggtggata ttgggcgtgt tgtaccaagt atagggcaat actttcgaca
     3001 ccggcatctt gcctttaagc atcagataca gccggcattt ccagta
//
LOCUS       DASUEG010000630         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_191738, whole genome shotgun sequence.
ACCESSION   DASUEG010000630 DASUEG010000000
VERSION     DASUEG010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191738"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="VFX11_11860"
     CDS             complement(<1..2071)
                     /locus_tag="VFX11_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4939363.1"
                     /translation="MQRKLNSRLSNKLFFAVMGTAMLLGLLLSVVQIMLDARTARKAL
                     EQSTQEILAMMREPATQAAFNLDRVMAQQVVEGLFQNQAVFYAAISIPGEPPLASAFR
                     DRQRIRYRSLMNSIFGQDLSYSLELKGYSPTDNTSVVLYGNLEVSIDPANSALDFLER
                     GQTLFISGLLQSVFFGSVLYLIFQGLIARPMTTLLGSLEDIDPMRPSQSKLDAPLGHE
                     DDELGAWVHKINDLFKAIEKYNSKRRVAEAHVERLSNYDMLTELPNRNLLLRRIESSI
                     QDANSQNDMFAVLYCALDDFKSVNLLHSYHAGDKLLLMMADRFRSELDPAQTVARVGG
                     DVFAIVLPGLTSHYQAADVAQRILDNVRRPFRLDNNTIKVSATIGITMYPHDAATAEQ
                     LLKNAENVMQLAKSEGGNRYQFYVANVDQKIRAAKVLEKQLATAVDDGQLELVFQPQV
                     NLLTGEVRGAEALVRWHHPERGLVPPNEFIPLAERTGAIVAIGEWVLQASCQCLRKWH
                     SMGITHLTISVNVSPVQLHQSDIVTCVREALEASAVPPQHLVLELTETAVMDNVDSAI
                     RLLKQIKALGVQLAIDDFGTGYSSLSYLKKMPMDEIKIDRTFVQDMLEDPDNGTIVDA
                     IIQLGHSLNLSVIAEGTETLDQIRYLQECECDMAQGYYYSQPLSESAFIALLQEQRGQ
                     IRNATQRA"
     gene            2286..2843
                     /locus_tag="VFX11_11865"
     CDS             2286..2843
                     /locus_tag="VFX11_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939364.1"
                     /translation="MRWLVLLASLLSFPLQAAGNDDAQETVESSVPPVPASRPPVSDK
                     KAREELQRLMDNGLSMLAQELVSTGTFYPFAAVLGNDNEIRLVGISAAERNAAPDAAL
                     AALVKKIKTLAAERRVRAAAYFMDYVAQRADTGYSQAGIRVELNHRHPDALSVFVPYS
                     VTSDKKLRLMTPQYRPGKNLTFDMK"
     gene            2845..3684
                     /locus_tag="VFX11_11870"
     CDS             2845..3684
                     /locus_tag="VFX11_11870"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939365.1"
                     /translation="MISTSLEKLQSALRKGMFNGMKTLGHRPGRVIRDLHYGREPEQK
                     LDIYLPAGDDRGAQLVFFHGGSWRSGHKDEYAFLGSALAAFGIRCAVVGYRLFPQVRY
                     PAFVEDAAHAVAWLQREGSRYGFSAGPLFLMGHSAGAHIACLLALDERYRTLAHLDAA
                     SIRGVIGLSGVYRFRPENSELHSAIFASAGPDFETVKPINYVGPNQVPLLMLHGSKDT
                     LVGYKTAQRMYEAAVAAGQKASLHAQKGYGHVRPLFDFLPFMPNHQKTMALLLAFMTE
                     HRT"
BASE COUNT          717 a         1099 c         1155 g          784 t
ORIGIN      
        1 cggcccgctg ggtggcgttg cggatctggc cgcgctgttc ctgcagcagg gcgataaacg
       61 ccgactccga cagcggctgg ctgtagtaat agccctgcgc catgtcgcat tcgcattcct
      121 gcagatagcg gatctggtcc agagtctcgg tgccttccgc gatcaccgac aggttcaagc
      181 tgtggcccag ctggatgatg gcatcgacga tagtgccgtt gtcgggatct tccagcatgt
      241 cctgcacgaa ggtgcggtcg atcttgattt cgtccatcgg catttttttc aggtagctca
      301 gtgacgagta tccggtaccg aaatcatcga tggccagctg cacgccaagc gccttgatct
      361 gcttaagcag acggattgcg gaatccacgt tgtccatcac cgcggtttcc gtcagttcca
      421 gcaccagatg ctggggcggc accgcgctgg cttccagggc ctcgcgcacg caggtgacga
      481 tgtcggactg gtgcagttgc acagggctga cgttcacgga tatcgtcagg tgcgtgatgc
      541 ccatgctgtg ccacttgcgc aggcactggc acgacgcctg cagcacccat tcgccaatgg
      601 cgacgatcgc gccggtccgt tccgccagcg gaatgaattc gttgggcggt accagcccgc
      661 gttccggatg gtgccagcgt accagcgctt cggcgccacg cacttcgccg gtgagcaggt
      721 tcacctgcgg ctggaaaacc agttccagct ggccgtcgtc caccgccgtg gccagctgct
      781 tttccagcac cttggcggcg cggattttct ggtccacatt cgccacataa aactggtagc
      841 ggttgccgcc ttcggatttg gccagctgca tcacattctc ggcgtttttc agcagctgtt
      901 cggccgtggc ggcatcatgg ggatacatgg tgatgccgat ggtggcagac accttgatcg
      961 tattgttgtc gagccggaac ggccgccgca cattgtccag gatgcgttgc gcaacatccg
     1021 ccgcctgata atggctggtc aatcccggca gtacgattgc aaacacatcg ccgccgaccc
     1081 gggccacggt ctgggcgggg tccagttcgg aacggaaacg gtcggccatc atcagcagca
     1141 gtttgtcgcc ggcatgatag gaatgcagca ggttaactga cttgaaatca tcgagcgcgc
     1201 agtagagcac cgcgaacatg tcattctggc tgttggcatc ctggatcgag ctttcgatcc
     1261 gccgcagcaa cagattgcgg ttgggcagtt cggtgagcat gtcgtaattg ctcaggcgct
     1321 ccacatgggc ttcggccacc cggcgcttgc tgttgtattt ttcgatcgcc ttgaacagat
     1381 cgttgatctt gtgcacccag gcgcccagct cgtcgtcttc gtgacccagc ggcgcatcca
     1441 gcttgctctg cgacggacgc atgggatcga tgtcttccag cgatcccagc aatgtggtca
     1501 tggggcgggc gatcaggccc tggaaaatca ggtacagcac cgagccgaag aacacagact
     1561 gcaacaggcc gctgatgaac agggtctggc cgcgttcgag aaaatccagg gcggagttgg
     1621 cagggtcgat gctgacttcc aggttgccat acagcacgac gctggtgtta tcggtgggcg
     1681 aataaccttt cagttccagg ctgtaactca gatcctggcc gaagatggaa ttcatcagtg
     1741 agcggtagcg gatgcgctgc ctgtcgcgga aggctgaggc cagtggcggt tcgcccggaa
     1801 tggagatggc ggcgtagaat acggcctggt tctggaacag cccctccacc acctgctgcg
     1861 ccatgacgcg atccaggttg aacgcggcct gggtggccgg ctcccgcatc attgccagga
     1921 tttcctgggt gctctgttcc agcgccttgc gtgcggtgcg ggcgtcgagc atgatctgca
     1981 ccacgctcag caacaggccc agcagcatcg cggttcccat gacggcgaag aacagtttgt
     2041 ttgacaggcg gctattcagt ttccgctgca tgcagagatc cgctttcccg ctatcattgt
     2101 tgttggaaat agagttatta aaatttcgct tgttaaaacc agtattgaat ataaccccag
     2161 gcaagtcaaa aattttggcc agtaaatcaa caggtaggac cattccctgt ccgggaccgg
     2221 atcacacagg tgtgcggata agcatgaaca tcaatgatcg cagttggagt tggccggtac
     2281 ggtggatgcg atggctggtg ctgctggcgt cgctgctgtc attccccctg caggcagccg
     2341 gcaatgacga tgcgcaggaa acggtggaat ccagtgtgcc gccggtacct gccagccggc
     2401 cgccggtgtc cgacaagaag gcacgcgagg agctgcagcg gctgatggac aatggtctgt
     2461 caatgctggc gcaggaactg gtgtccaccg gcaccttcta cccttttgcg gccgtgctgg
     2521 gcaacgacaa tgaaatccgg ctggtgggaa tatccgccgc cgagcgcaat gcagcacccg
     2581 atgccgcgct ggcggcactg gtgaagaaga tcaagaccct ggctgcagaa cgtcgcgtgc
     2641 gtgccgcagc ctatttcatg gattatgttg cgcagcgcgc cgacaccggc tattcccagg
     2701 ccggcattcg ggttgaactc aatcaccgcc atccggacgc gctttcggtg ttcgtgccct
     2761 acagcgtaac atcggataaa aagctgcgtc tgatgacacc ccaataccgc cctggcaaaa
     2821 acctcacatt cgacatgaag tagtatgatc agcacatccc tggaaaaact gcagagcgcc
     2881 ctgcgcaagg gcatgttcaa cggcatgaaa accctcggcc accgtccggg gcgcgtcatt
     2941 cgcgacctgc attacggccg cgaaccggag cagaaactcg acatctacct tcccgccggc
     3001 gacgaccgtg gcgcgcagct ggtgtttttt cacggcggca gctggcgctc cggccacaag
     3061 gatgagtatg cgttccttgg cagcgccctg gcggcgttcg gcattcgctg cgcggtggtg
     3121 ggttaccggc tgtttccgca ggtgcgttat ccggcttttg tggaagacgc ggcgcacgca
     3181 gtggcctggc tgcagcgtga gggttcccgt tatggattct ccgccggtcc gctgtttctg
     3241 atggggcatt ccgccggggc gcatatcgcc tgcctgctgg cactggacga acgttatcgc
     3301 acacttgcgc atctggatgc cgcgtcgatc cggggcgtga tcggattgtc cggcgtgtac
     3361 cgtttccggc cggaaaattc cgaactgcat tccgccatct tcgccagcgc cgggccggat
     3421 ttcgaaacgg tgaaaccgat caattatgtg gggccgaacc aggtgccatt gctgatgctg
     3481 catggcagca aggacactct ggtgggctac aaaaccgccc agcgcatgta tgaagcagcc
     3541 gtggcggcag ggcagaaggc cagcttgcac gcgcagaaag gttacggcca cgtgcggccg
     3601 ctgttcgact tcctgccgtt catgcccaat caccagaaaa ccatggcgct gctgctggcg
     3661 ttcatgacgg agcatcggac gtgaaaatcc tggttaccgg tgccaacgga tttgtgggag
     3721 ggcgttacgt gcgccatctg gcgcagaccc gtccg
//
LOCUS       DASUEG010000631         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_265515, whole genome shotgun sequence.
ACCESSION   DASUEG010000631 DASUEG010000000
VERSION     DASUEG010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265515"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3017)
                     /locus_tag="VFX11_11875"
     CDS             complement(<1..3017)
                     /locus_tag="VFX11_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX4939366.1"
                     /translation="MAAGLIDELESFDARFFGIAPREIESMEPQQRLVLEHAWTALEN
                     AGYSPAAMNGTRTGVFVGVGANEYIRACATGAREEDIMFIPTGNALNVIAGRVAFNLG
                     LQGPCMTIDTACSSSAVATHLACQSLRNGECEMALASGVNAMVMPETFVALSKAHMLS
                     KEGRCKTFDESADGYVRGEGVGVLVLKRLSDAERDGDNIIAVIKGSAINQDGRSSSLT
                     APNGPQQQAVIRAALANAGLKAEEVDWVETHGTATPLGDPIEVQSLEAVYCGHRSAEN
                     PLIISSVKTNIGHLESAAGVSSIMKAALSLQHGVIPKHLHFNKFNPHIAVDQSKFFIP
                     TEKMEWKGGEKKRRVGISSFGFSGTNVHMILEEAPVRKEVINATERNSHVLTFSAKSE
                     ASLNGLAKRYAEFLESDLLNSKPAAFADVAFTANTARAQFEFRAAVVANDAASAADKL
                     KQLAEGHTSVGAFRGEAKPVTGTAWLFTGQGSQYAGMAKELYDTNPAFKQKLDACEKL
                     FEAETAESLLGILWGDQTAEIDNTRYTQPAIFALEYALASHWQSLGLKPTVMVGHSVG
                     EYAAAVIAGIMSLDDAMKLIVARGKLMVELCEKGDMAAVLTTQDKVEEIIAGNSAVQL
                     AACNAPGNTVVSGTADGIKAVLQKASSLDVDARLLTVSHAFHSNMMQPMLDAFRKVAE
                     SISYKPATLSIVSTVSGRANQGEMSTAGYWVEHVKRPVLFVNAVKELPALKVNVCLEV
                     GPGATLTSLAQQTLGQDGFTFVHSLRAKQDASRQFNLAAAQLYSRGVDINWKGFDAPY
                     ARQRVAAPAYAFDRKRYWLGDNDNGMVSAGGNMGQVTENVLMMLQSPMSDDYYFENSF
                     GGRHPFNLDDHRLYEVVVAPGAFHVANTILCARDAFGEKPVKLDDVVFPEPLIIEGDK
                     KRRLHYGFKKIASKDSVDQYEVKGFSRPDQKGSEKDWTMHCSMNVALCDGPDATQVLT
                     PQDIEQIQGRATIAFDGQMFYDEMWKVGYQLGS"
BASE COUNT          563 a          929 c          879 g          665 t
ORIGIN      
        1 tgcgagccca gctggtaacc cactttccac atttcgtcat agaacatctg gccgtcaaag
       61 gcgatagtgg cacggccctg gatctgctcg atatcctgcg gcgtcagcac ctgggtcgca
      121 tcggggccat cgcacagcgc aacgttcatg ctgcagtgca tggtccagtc tttttccgac
      181 cctttctggt ccggacggct gaaacctttt acttcgtact ggtcgacgct gtccttgctg
      241 gcgattttct tgaagccgta atgcaggcga cgcttcttgt caccttcgat gatcagcggc
      301 tcggggaaca ccacatcgtc cagcttcacc ggcttctcgc cgaaggcatc gcgggcgcag
      361 aggatggtgt tggccacgtg gaatgcaccg ggcgcaacca ccacttcata cagacggtga
      421 tcatccaggt tgaacggatg acggccaccg aacgagttct cgaagtagta gtcgtcgctc
      481 atgggcgact gcagcatcat cagcacgttc tcggtgacct gacccatgtt gccgccggcg
      541 gagaccatac cgttgtcgtt gtcacccagc cagtaacgct tgcggtcgaa ggcgtaagcc
      601 ggcgctgcga cgcgctggcg ggcgtaaggc gcatcgaaac ctttccagtt gatgtcgaca
      661 ccacgtgagt acagttgcgc ggcagccagg ttgaactggc ggctggcatc ctgtttcgcg
      721 cgcaggctgt ggacgaaggt gaagccatcc tgacccagcg tctgttgtgc caggctggtg
      781 agcgtggcgc cggggcccac ttccagacac acattcactt tcagtgccgg cagttctttc
      841 accgcattca cgaacagtac cgggcgcttg acgtgctcta cccagtaacc ggcggtggac
      901 atttcaccct ggttggcgcg accactgacg gtggacacga tcgacagcgt ggccggcttg
      961 tagctgatgg attccgccac cttgcggaag gcgtccagca tcggctgcat catgttggaa
     1021 tggaaagcgt gcgacacggt gagcaggcgc gcatccacat ccagcgagga cgctttctgc
     1081 aagaccgcct tgataccgtc ggcagtgccg gacaccacgg tattacccgg ggcattgcac
     1141 gctgccagct gcacggcgct gttgccggcg atgatttctt ccaccttgtc ctgcgtggtc
     1201 agcacggcgg ccatgtcgcc tttctcgcac agttccacca tcagtttgcc gcgtgccacg
     1261 atcagtttca tcgcgtcatc caggctcatg atgccggcga ttaccgccgc cgcatattcg
     1321 cccacgctgt ggccaaccat gacagtgggt ttcaggccca ggctctgcca atggcttgcc
     1381 agtgcgtatt ccagcgcgaa aatcgccggc tgggtgtagc gggtattgtc gatttccgcc
     1441 gtctggtcgc cccacagaat gcccagcagg gattcagcgg tttcggcttc aaacagcttt
     1501 tcacaggcat ccagtttctg cttgaacgcc ggattggtgt cgtacagttc cttcgccatg
     1561 ccggcgtact gcgacccttg gccagtgaac agccatgctg tgccggtgac cggcttggct
     1621 tcgccccgga aagcaccgac gctggtgtgc ccttctgcca gctgcttcag tttgtcggca
     1681 gcggacgcag catcgttcgc caccacagca gcgcggaatt caaattgcgc gcgggctgta
     1741 ttggcagtga acgccacgtc ggcaaatgcc gccggcttgc tgttgagcag atcgctttcc
     1801 aggaactcgg cataacgctt ggccagaccg ttgagtgacg cttcggattt cgccgagaac
     1861 gtcagtacat ggctgttgcg ttcggtggcg ttgatcactt ccttgcgcac cggtgcttct
     1921 tccagaatca tgtgcacgtt ggtgccgctg aaaccgaacg agctgatacc gacgcgacgc
     1981 ttcttctcac cgcctttcca ttccatcttc tcagtaggaa tgaaaaactt ggactgatcc
     2041 actgcgatat gcggattgaa cttgttgaaa tgcaggtgct tgggaatcac gccatgctgc
     2101 agcgacagcg ctgctttcat gatgctggat acaccggcag cagactccag gtgaccgatg
     2161 ttggttttca ccgagctgat gatcagcggg ttttccgcgc tgcggtgacc gcagtacaca
     2221 gcttccagcg actgcacttc gatcggatca cctagcgggg tggcagtacc gtgggtttcc
     2281 acccaatcca cttcttcggc tttcagaccg gcattggcca gcgccgcgcg aatcaccgcc
     2341 tgctgctgcg gaccattcgg cgcagtcaga ctgctgctgc ggccatcctg gttgatcgcg
     2401 gagcccttga tcaccgcaat gatgttgtcg ccatcccgct cggcatccga caggcgcttc
     2461 aacaccagca cgcccacgcc ttcgccgcgc acgtaaccgt cggcactttc gtcgaaggtt
     2521 ttgcagcggc cttctttcga cagcatgtgc gctttcgaca atgccacgaa ggtttccggc
     2581 atcaccatgg cgttcacacc actggccagc gccatttcgc actcgccgtt gcgcaggctc
     2641 tggcaggcca ggtgagtggc aaccgcggac gatgagcacg cagtgtcaat ggtcatacag
     2701 gggccttgca gacccaggtt gaaggcaaca cggccggcga tcacgttgag cgcgttgccg
     2761 gtggggatga acatgatgtc ttcttcacgg gcaccggtgg cgcaggcgcg gatgtattca
     2821 ttggcgccaa caccgacgaa cacaccggtg cgggtgccgt tcattgcggc cggggaataa
     2881 ccggcgtttt ccagcgcggt ccaggcatgt tccagcacca gacgttgttg cggctccatg
     2941 ctttcgattt cacgtggtgc aataccaaaa aagcgtgcat caaaagattc caactcgtcg
     3001 atcaaaccgg ccgccaaggt ataggctttt ccgggt
//
LOCUS       DASUEG010000632         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_315072, whole genome shotgun sequence.
ACCESSION   DASUEG010000632 DASUEG010000000
VERSION     DASUEG010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315072"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFX11_11880"
     CDS             complement(<1..1071)
                     /locus_tag="VFX11_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4939367.1"
                     /translation="MNANPYDQLPYRCQPIEWTAPERLALASLLHQGPVPDLRRYRVL
                     ELGCGDGSNLLPLAYYRPQASFMGIDGASTQIRLAQRRQQQLQVPNVEFMALDFLQAN
                     DRLDGTFDFIIAHGVFSWVPDAVRDALFALCQQRLADDGLLYLNYNTRPGWNVRGMVR
                     DYLLGLSDATQPLLLRAQSAQAAAAKMAQSLATEQHPFSQLLANEFQFVCDNHVSYVA
                     HEYLAEHNHAYWRSEFLALAAGYGFHCVADADYNYASGRVDAALPARLISMGLAGREL
                     KDTEDLLSYRQLHTPILSRQRAKAPQPEHAILRQLQVASCLQPLGAHQPFWFEHPNGY
                     QVEAKDALMAAALNRLATIWPDP"
     gene            complement(1085..1552)
                     /locus_tag="VFX11_11885"
     CDS             complement(1085..1552)
                     /locus_tag="VFX11_11885"
                     /inference="COORDINATES: protein motif:HMM:NF040117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HEX4939368.1"
                     /translation="MSVFENPQPAQLRSPVYGVVVGQYLGLNPQQQPLVVFPGQEGTA
                     ACVARTIAGLFNVAFGAEVLLQFDQGDVDKPIILGVIQQPETWTQQIAGHQLEVDADG
                     QRLQIQAQEQLVLRCGAASITLTKAGKVLIKGTYLSSHASGTQRIKGGCVQIN"
     gene            complement(1582..>2603)
                     /locus_tag="VFX11_11890"
     CDS             complement(1582..>2603)
                     /locus_tag="VFX11_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX4939369.1"
                     /translation="APVTVPVRPPRSSVKRQMPGPQTAKVMTLSASASMADADPQRMV
                     RVQFPWDGEHNSCKLRVLQGYAGSGWGGSFVPREGQEVLVDFINGDPDRPIVVGALYN
                     KDNQGPKYTSTQSGWLTQSGNANELRFDDKGGAEEVYLKAGKDWNYVVANNETGQVQN
                     AQSLTVSSTRTVAVGSNESKTVAANQTLAVTQNQSITVGGNKTETIAINCAETIGVAK
                     ELTIGGLYQVTVGAAMNETVAAIKAEEVGASRTLAVALNMSENVGKNRSIKVGDNSSH
                     SAKKIVITAEDELTVKVGKATLVMKKDGSVALNGKDIEIKGSGKINVKASSDVVIKGS
                     KVGVN"
BASE COUNT          499 a          840 c          707 g          557 t
ORIGIN      
        1 cggatcgggc cagatagttg ccagccggtt cagcgcagcg gccatcaggg catccttcgc
       61 ttccacctga tagccgttgg gatgctcgaa ccagaatggc tggtgggcac ccagcggctg
      121 cagacaggac gccacctgta attgccgcag gattgcgtgc tcaggttgag gggcctttgc
      181 tcgctgccgg ctcagaatcg gtgtgtgcag ctgccggtag ctgagcagat cttccgtatc
      241 tttcagttcg cgcccggcaa gtcccatcga gatcaagcgg gcgggcaacg ccgcatccac
      301 ccgccccgat gcatagttgt aatccgcatc ggcaacacaa tggaaaccgt acccggcagc
      361 cagcgccagg aattcactgc gccagtacgc atggttatgt tccgccagat actcgtgggc
      421 gacgtagctg acatgattgt cgcatacgaa ctggaattcg ttggccagca gttgcgagaa
      481 cggatgctgc tccgtagcaa gggactgtgc cattttcgct gcggcggctt gcgccgactg
      541 ggcacgcaat aacaagggct gagtcgcatc actcaatccc agcaaataat cccgcaccat
      601 gccgcgcaca ttccagcccg gccgggtgtt gtaattcaaa tacagcaaac cgtcgtcagc
      661 aaggcgctgc tggcataacg caaacaacgc atcgcgcacc gcatccggta cccaggaaaa
      721 tacgccgtgg gcgatgatga aatcgaatgt gccgtcgagc ctgtcattgg cctgcagaaa
      781 atccagcgcc atgaattcca cattgggcac ttgcaactgc tgctggcgac gctgtgccaa
      841 ccggatctgc gtacttgcac cgtcaattcc cataaagctg gcctgcggcc ggtaataggc
      901 cagcggcaac agattactgc cgtcgccaca acccagttcc agcacccgat accggcgcag
      961 atccggcacc ggcccctggt gtaacaggga cgccaatgcc agccgctccg gagccgtcca
     1021 ctcaatgggt tggcagcggt aaggcaattg atcgtaggga ttggcattca tagggatttg
     1081 cacgtcagtt gatttgcacg cagccgccct tgatacgctg ggttccgctg gcgtgggacg
     1141 acagataagt gcccttgatc agcaccttgc cggccttggt gagcgtgatg ctggcggcac
     1201 cgcagcgcag taccagctgc tcctgagcct gaatctgcag acgctggccg tcggcatcca
     1261 cctccagctg gtgaccggca atctgctgcg tccaggtttc tggctgctgt atcacaccca
     1321 gaataatggg tttgtcgaca tccccctgat cgaactgcaa cagcacctcg gcaccaaacg
     1381 caacattgaa tagcccggcg atggtgcggg ccacacaagc tgccgttcct tcctggccgg
     1441 ggaacaccac cagcggctgc tgctgcggat tcaatcccag atactgcccg acgaccacgc
     1501 catacaccgg gctgcgcaat tgcgccggct gcggattttc gaatacggac atgccttcct
     1561 ccttgagcgg ttcatccgct gtcagttcac gcccaccttg ctgcctttga tcacaacatc
     1621 gctgctggct ttgacattga tcttgccact gcccttgatc tcgatgtcct tgccgttgag
     1681 cgcaacgctg ccgtcttttt tcatgaccag cgtagccttg cccactttca cagtgagttc
     1741 atcttcagca gtgatcacaa ttttcttggc cgaatggctg ctgttgtcgc ccaccttgat
     1801 gctgcgattc ttgccgacgt tctcggacat gttcagcgcc accgccaggg ttcggctggc
     1861 acccacctct tccgccttga tcgccgccac ggtttcgttc atggcggcac ccaccgtcac
     1921 ctggtacagg ccgccgatgg tcagttcctt ggcaacaccg atggtttcgg cgcaattgat
     1981 ggcgatggtt tcggttttgt tgccgcccac ggtgatcgat tgattctggg taacggcaag
     2041 agtctgattg gcagcgacgg ttttcgattc attggacccc actgcaaccg tgcgcgtgct
     2101 gctgaccgtc aacgactgcg cgttctgcac ctggccggtt tcattgttgg ccaccacata
     2161 attccagtcc ttgcccgcct tcagatacac ctcttccgcg ccacccttgt catcgaagcg
     2221 cagctcgttg gcattgccgc tctgcgtcag ccagccagac tgggtggacg tatatttcgg
     2281 cccctggttg tccttgttat acagcgcacc caccacaatc gggcgatccg gatcgccgtt
     2341 gataaaatcc accagcactt cctgcccttc ccgcggcacg aaactcccgc cccagccact
     2401 gccggcataa ccctgcagca cccgcagctt gcagctgttg tgctcgccgt cccagggaaa
     2461 ctgcacccgc accatgcgct gcggatctgc gtcggccatt gatgccgatg cgctcaacgt
     2521 catgacctta gccgtctgcg gccccggcat ctggcgcttc acacttgatc gcggcggtcg
     2581 cacaggaacg gtcacaggag cac
//
LOCUS       DASUEG010000633         6575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1789484, whole genome shotgun sequence.
ACCESSION   DASUEG010000633 DASUEG010000000
VERSION     DASUEG010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789484"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFX11_11895"
     CDS             <1..235
                     /locus_tag="VFX11_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939370.1"
                     /translation="EPFFTTKEPGKGTGLGMSLVYSIIEEHYGHIAIHSPLDSAGGGT
                     RVTITLPRYPVLRLETPDADIHLEETHEPHPDR"
     gene            213..1613
                     /locus_tag="VFX11_11900"
     CDS             213..1613
                     /locus_tag="VFX11_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4939371.1"
                     /translation="MSHILIVEDESVIRTALRRLLERNGYQVSEAGAVQDAVDNFKLV
                     DFDLIVTDLRLPGAPGTDIIPLADGVPVLVMTSYASLKSAVESMKLGAVDYIAKPFDH
                     DEMLITVERILKQKALERENQALKSQISRDFPASGMIGECDAMQALFRNIQKVAPTDS
                     TVLVLGESGTGKELVARALHEQSLRREGPLISVNCAAIPETLIESELFGHEKGAFTGA
                     NANRKGLVEAADAGTLFLDEIGELPLEAQARLLRLLQEKEVRRVGSVQSKTVDVRLVA
                     ATHRNLKNLAREGRFREDLYYRLHVVELRLPPLRERGKDVEKLAKELLVRTARKMAKP
                     ELTFSQDALDAIGQYRWPGNVRELENAIERAVILVDGQFITPDLLAIDLSPQYELEED
                     YPLPTPSQHFRANSDIADEPQEDLSLEDYFQRFVLEHQEQMTETELAQKLGISRKCLW
                     ERRQRLGIPRKKPFTR"
     gene            2620..3144
                     /locus_tag="VFX11_11905"
     CDS             2620..3144
                     /locus_tag="VFX11_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX4939372.1"
                     /translation="MPMPPSTADRARQLLSHLPPPSRVIASLHPILKSQARLIPFALQ
                     RRVMEKIMQEGFRDAIADGAFDFLQGRWLCLEITDLRLHWNITQGMHGPVMMASAVKP
                     DVCIRGRLRDFVALANQEVDPDTLFFRRRLQISGDTDLGLQVKNLMFGTEITGLPRAL
                     SRILQQLMEFSPGK"
     gene            complement(3207..4109)
                     /locus_tag="VFX11_11910"
     CDS             complement(3207..4109)
                     /locus_tag="VFX11_11910"
                     /inference="COORDINATES: protein motif:HMM:NF011991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX4939373.1"
                     /translation="MKISLGPVLYFWDRKTLLDFYARALETPVDIIYLGEVVCSKRRA
                     LNAADWIDLAADIARCGRQVVLSTLTLIEAESEIGYARNLVERFDGMIEANDYAVLNM
                     ARAQGRDICAGSSLNIYNSATLETLAQCGLKRWVPPLEMSGESLLSMLQNLARDGDRP
                     RIETEVFSYGRLPLAHSARCFTARFLNRPKDQCEFRCADYQDGIPLFSQEQQGLFQIN
                     GIQTQSFAHCNLLPYWRLLRDAGVDIMRISASSPRCLQKIAQLKNDVTGNTTGIPLAE
                     QDTCNGYWFGEPGMVSLPAVQGNL"
     gene            complement(4134..5129)
                     /locus_tag="VFX11_11915"
     CDS             complement(4134..5129)
                     /locus_tag="VFX11_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX4939374.1"
                     /translation="MELVCPAGNLPSVKAAVDSGADAVYIGLNNKTNARYFSGLNFSD
                     SNIEKAVQYIRDHGRKVFVAINTYPQEKNWPQWRDAVDTGASLGVDALIIADIGVMDY
                     AASRHPHIARHLSVQASATSLEALQFYHAQFGIRRAVLPRVLSLKQVERLAAKSPIEL
                     EVFGFGSLCIMAEGRCYLSSYVTGDSPNNCGACSPAHAVRWEESAHILETRLNNVLID
                     RFQPDEPAGYPTLCKGRFQVEQHLFHALEEPVSLNALDMLPQLADAGIAAIKIEGRQR
                     APAYIAKVARVWRQAIDCHARLGAGYVAQADWHRTLAELAEGSQTTLGAYHRPWQ"
     gene            complement(5195..>6575)
                     /gene="ubiD"
                     /locus_tag="VFX11_11920"
     CDS             complement(5195..>6575)
                     /gene="ubiD"
                     /locus_tag="VFX11_11920"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041599123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEX4939375.1"
                     /translation="YLEMTEVSDRILRIQGPALLFENPTGFNVPVLTNLFGTGRRIAL
                     GLGLKDVSELRQLGEALAHLKDPAPPASLRGIFEQLPMLKNVMNMAVKELRSGPCQEV
                     VLEKDDVDLAQWPIQTCWPEDAGPLITWPLVITRGPNKPRLNIGIYRQQVIGRNRVIM
                     RWLSHRGGAIDHAEWQRKHGAQPFPVAVVIGADPATILAAVTPIPDTLSEFSFAGLLR
                     GQKTALVQCISHDLWVPATAEIVLEGFIYPGDCADEGPFGDHTGYYNECASFPVFTIE
                     RITHRHRPIYHSTYTGRPPDEPAILAAAMNEMFVPILCKQFPEIVDFYLPPEGCSYRM
                     AVVSIRKQYPGHARRIMMGVWSMLRQFMYTKFIVVVDEDIDVRNWQDVIWAMTTRMDP
                     ARDTVMVEHTPIDYLDFASPVSGLGSKMGLDATHKWAGETQREWGRVIRRDPAIVQKI
                     DLLWNRLGL"
BASE COUNT         1493 a         1982 c         1809 g         1291 t
ORIGIN      
        1 cgaacccttc ttcaccacca aggagccggg caaaggcacc ggactgggaa tgtccttggt
       61 ttacagcatc atagaggaac attatgggca cattgccatc cacagcccgc tggattccgc
      121 cggcggcggc acccgtgtta ccataaccct cccccgttat ccggtgttac gcctggaaac
      181 acctgacgcc gacatccatc tcgaggaaac gcatgagcca catcctgatc gttgaagacg
      241 aatccgtgat ccgcactgca ctgcgccgcc tgctggaacg caacggctac caggtcagtg
      301 aagcgggcgc ggtacaggat gcggtggaca acttcaagct ggtggatttt gacctgatcg
      361 ttaccgacct gcgcctgccc ggcgcccccg gcaccgacat catccccctg gccgacggtg
      421 tgcccgtgct ggtcatgacc agctatgcca gcctaaaatc cgccgtggaa tccatgaaat
      481 tgggcgcagt ggattacatc gccaagccgt tcgatcacga cgaaatgctg atcacggtgg
      541 agcgcatcct gaaacagaag gcactggagc gcgaaaacca ggccctgaaa tcccagatca
      601 gccgcgattt ccccgcctcg ggcatgatcg gcgaatgcga tgccatgcag gccctgttcc
      661 gcaatatcca gaaagtagcc cccaccgatt ccaccgtgct ggtactgggg gaatccggta
      721 ccggcaagga actggtggcc cgcgccctgc acgaacaaag tctgcgccgc gaaggccccc
      781 tcatttcagt caactgcgcc gcgattcccg aaaccctgat cgaatccgaa ctgttcggcc
      841 atgaaaaagg tgccttcacc ggtgccaacg ccaaccgcaa ggggctggtg gaagcggccg
      901 acgccggcac cctgttcctg gacgagatcg gcgaactgcc gctggaagcc caggcccgcc
      961 tgctgcgcct gctgcaggaa aaggaagtgc gccgggtggg atcggtacag tccaagactg
     1021 tcgacgtgcg gctggtggcc gccacgcatc gcaacctgaa gaacctggcc cgggaaggcc
     1081 ggtttcgcga agacctttat taccggctgc acgtggtgga actgcgcctg ccacccttgc
     1141 gcgagcgcgg caaggatgtg gagaaactgg caaaggaact gctggtgcgc accgcccgca
     1201 agatggccaa gccggaactg accttttccc aggatgcgct ggacgccatc ggccagtatc
     1261 gctggcccgg caacgtgcgc gagctggaaa acgccatcga gcgggcggta atcctggtgg
     1321 acggccagtt cattaccccg gacctgctgg ccatcgacct gagcccgcag tacgaactgg
     1381 aagaagatta ccccctgccc acccccagcc agcacttccg cgccaacagc gacattgccg
     1441 atgaacccca ggaagacctg tcgctggaag actatttcca gcgctttgta ctggaacacc
     1501 aggaacagat gacggaaacc gagctggcgc agaagctggg catcagccgc aagtgcctgt
     1561 gggaacgccg ccagcgcctg ggtattcccc gcaagaagcc tttcacgcgc tgatcaacgc
     1621 cctctccccg gggtttgcac cggctttacc agcctggcag ccccgtcaag ttatcgacac
     1681 tcacgccagc tcaaaaaacg atcaaaacgt tgacgctcca ccgtcatccc actgaccaat
     1741 cctgaagcag ccgatactgt ccgccacgcc ggttccggtg acgtttgcgc taaataaatg
     1801 aaatgtggat ggattcaatt gctctgtaac agagtgttac cagtgatacc gcagggggga
     1861 gtgttaccgg ggtacacaca aaaagaaaca gtttccccca gtaacggtaa cagatagctg
     1921 ccaagttacc ggcgttacac aaaataacat tgttaagtta atgatatata tcgcttttct
     1981 attttcgccc cgacttggca cgcaccctgc tatatattcg gtgtacctaa ggcagacaac
     2041 aaaaataata agaaggttct accgaaggtc agaaaacaac aataacaata ataaagtgct
     2101 aaacggtggt tatataaaaa gtggcagcat gtggtagccg cgaagttgaa gccagtacaa
     2161 aaacaataaa aatcggccca acctcctcta tcccatctgt cacagccacc tcaaaaaata
     2221 ataaaaataa aaaaaacagg caataaagag catcataaaa ataaaaaagc gattggcgaa
     2281 aaaaacaaaa ataagacacg ctgaaaaaca acaataagag tcggcactaa tggaccgggc
     2341 ctacgggccc ggtccaaaga acagaattct ccttcaataa gaaaaagaaa aaacatgaag
     2401 cacaagggaa agcgaaagct ttcccttctt gcttttcaga gtccgcattt tcctattccc
     2461 cgccagcatc ttacttgcct ccacctgtgc gtccacgcta cctccttttc cacgctgaat
     2521 cgccgcacgc catagcgcgc cgctgacacg ggtcagtaaa aattctgcag cacgcctcta
     2581 cagtgagcgc tcccgcaatc cggcaggaga aacccgatca tgcccatgcc accctccaca
     2641 gcagaccgcg cgcgccagct gttgtcgcac ctgccaccgc cgtcccgcgt cattgcaagt
     2701 ctgcatccca ttctaaaatc ccaggcacgg ctgattccgt ttgcgttgca gcgccgggtg
     2761 atggaaaaaa tcatgcagga aggatttcgc gacgccattg cggacggcgc ttttgatttt
     2821 ctgcagggac gctggctatg cctggaaatc accgatttgc gcctgcactg gaacattacc
     2881 caaggcatgc acggaccggt gatgatggcg agcgcggtga aaccggatgt ctgcatccgg
     2941 ggccgcttgc gggatttcgt ggcactggcc aatcaggaag tggacccgga taccctgttc
     3001 ttccgccgtc gcctgcagat cagcggcgac accgatctgg gcctgcaggt gaagaatctg
     3061 atgttcggca cggaaattac cggattgccg cgcgcgctca gtcgtatact gcagcaactt
     3121 atggaatttt cgccgggaaa atgaagctgc agcagagcgg atcagcccag ggaacctctg
     3181 aataacgtag caagcgcagt agttattcag aggtttcctt gcactgccgg caagctcacc
     3241 atgcccggct cgccaaacca gtagccattg caggtatcct gttctgccag cgggatgccg
     3301 gtggtattcc ccgtcacgtc atttttcagc tgcgcaattt tctgcaggca ccggggcgat
     3361 gacgcgctga tgcgcatgat atcgacaccc gcgtcccgca gtaaccgcca gtatggaagc
     3421 aggttgcagt gcgcaaagga ctgggtctgg atgccattga tctggaacag accctgctgc
     3481 tcctggctga aaagcggaat accgtcctgg taatcggcgc agcggaattc gcactggtcc
     3541 ttgggccggt tgagaaagcg tgcggtgaag cagcgggcgg aatgggccaa tggcagccgg
     3601 ccgtagctga atacttcggt ttcgatacgg gggcggtcgc cgtcgcgtgc gaggttttgc
     3661 aacatcgaca gcagggattc gccgctcatt tccagcggcg gcacccagcg tttcagaccg
     3721 cactgcgcca gcgtttccag cgtggcgctg ttgtagatgt tcaggctgga gccggcgcag
     3781 atatcgcgcc cctgcgcacg cgccatgttc aacaccgcat aatcgttggc ttctatcatg
     3841 ccatcgaaac gctccaccag attgcgggcg taaccgattt ccgattcggc ctcgatcagc
     3901 gtgagcgtgg acagcaccac ctgcctgccg caacgcgcga tatccgccgc aaggtcgatc
     3961 cagtctgccg cgttcaacgc gcggcgtttg gagcagacga cttcaccgag ataaatgata
     4021 tccaccggcg tttccagcgc gcgggcataa aaatccagca gggtttttct gtcccagaaa
     4081 tacagcaccg gtccgagcga aattttcatg gtggcgggtt ccatcagcga atgttattgc
     4141 cagggccgat gataggcgcc cagcgtggtt tgcgatcctt ccgccagctc cgccagcgtg
     4201 cggtgccaat ccgcctgtgc gacgtagccg gctcccaagc gcgcatgaca atcgatggcc
     4261 tggcgccaga cgcgcgccac ttttgcgata taggcaggcg cgcgctggcg gccttcgatt
     4321 ttgatggcgg cgatgccggc gtctgccaac tgcggcagca tatcgagcgc attgaggctg
     4381 accggctctt ccagcgcatg aaacagatgt tgctccacct ggaaacggcc tttgcacagg
     4441 gtgggataac ccgccggttc gtccggctgg aaacggtcga tcaaaacgtt gtttaagcgc
     4501 gtttccagta tgtgggcgga ttcttcccag cgcaccgcat gcgccggtga gcaggcgccg
     4561 caattgttgg gcgaatcccc cgtcacgtag gacgataaat agcagcgccc ctccgccatg
     4621 atgcacaggg aaccaaaccc gaagacttcc agttcaatcg gggattttgc tgccagccgt
     4681 tccacctgct tcagtgacaa cacccgtggc aacactgcgc gccggatacc aaactgcgcg
     4741 tgataaaact gcagggcttc cagactggtg gcggaagcct gcacggaaag atgacgcgcg
     4801 atgtgcggat gccgtgacgc cgcataatcc attacgccga tgtcggcaat gatcagcgcg
     4861 tcaacgccca ggctggcacc ggtgtccact gcatcacgcc attgcggcca gttcttttcc
     4921 tgcggatagg tgttgatggc gacaaacacc ttgcggccgt gatcgcgaat gtactgcacg
     4981 gctttttcaa tattgctgtc ggaaaaattc aggccggaaa agtaacgggc attggttttg
     5041 ttgttgaggc cgatgtagac cgcatcggcg ccgctgtcga cagccgcctt gaccgagggc
     5101 aggttgccgg ccggacagac cagctcgatc tgccgtgctg tcttcattcc gcacgtatcc
     5161 atttcccgca tttccatttc ccgcgttttt attttcacag tcccagcctg ttccagagca
     5221 aatcgatttt ctgcacgatg gcgggatcgc gacgaatgac gcgcccccat tccctttgcg
     5281 tttcgccggc ccacttgtgg gtggcatcca ggcccatctt ggaacccagc cccgataccg
     5341 gcgaggcgaa atccagataa tcgatgggcg tgtgttccac catcaccgta tcgcgggccg
     5401 ggtccatgcg ggtagtcatt gcccagatca catcctgcca gttgcgcacg tcgatatcct
     5461 cgtccaccac cacaatgaat ttggtataca tgaattgccg cagcatggac cacacgccca
     5521 tcatgatccg ccgcgcgtgt cccgggtatt gcttgcggat gctgaccacc gccatgcggt
     5581 aggaacagcc ctccggcggc agatagaaat ccacaatttc cggaaactgt ttgcacagga
     5641 tcggcacgaa catttcgttc atcgccgccg ccaggatcgc gggttcgtcc ggtggccggc
     5701 cggtgtaagt gctgtgataa atgggacgat gccggtgggt aatgcgttcg atggtgaaca
     5761 ccggaaacga cgcacactcg ttgtaatatc cggtgtgatc gccgaacgga ccttcatcgg
     5821 cgcaatcgcc aggatagatg aagccctcca gcacgatttc cgccgttgcc ggcacccaca
     5881 gatcgtgact gatacattgc accaacgcag ttttctgtcc gcgtagcagg ccggcaaagg
     5941 aaaattccga cagcgtatcg ggaatgggcg tgactgcagc gagaatcgtg gcgggatcag
     6001 cgccgatcac cactgccacg ggaaacggtt gtgcgccgtg cttgcgctgc cactccgcat
     6061 gatcaatggc gccgccacgg tgagacagcc agcgcataat cacccggttg cggccaatca
     6121 cctgctgccg gtaaatgccg atattcaggc gcggtttatt gggcccgcgt gtaatcacca
     6181 gaggccaggt gatgagtggc ccggcatctt ccggccaaca ggtctggatc ggccattgcg
     6241 ccagatcgac atcgtctttt tccagaacca cttcctggca gggcccgcta cgcaattcct
     6301 tcaccgccat gttcatgacg tttttcagca tcggcaactg ttcgaagatg cctctgagag
     6361 aagccggtgg cgcgggatct ttcagatggg ccagcgcttc acccagctgt cgcagctcac
     6421 tcacatcttt caagcccaga cccagcgcaa tgcgcctgcc ggtgccaaaa agattggtca
     6481 gcacgggcac attgaatccg gttgggtttt caaacagcag tgccggtccc tgtatgcgca
     6541 ggatacgatc cgacacttcc gtcatctcca gatac
//
LOCUS       DASUEG010000634         4776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2101812, whole genome shotgun sequence.
ACCESSION   DASUEG010000634 DASUEG010000000
VERSION     DASUEG010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101812"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="rplL"
                     /locus_tag="VFX11_11925"
     CDS             complement(<1..274)
                     /gene="rplL"
                     /locus_tag="VFX11_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX4939376.1"
                     /translation="MALSKDDILNAVAEMSVMDLVELISAFEEKFGVSAAAVAVAAGP
                     AAGGAAAAEEKTEFDVILASFGANKVGVIKVVREATGLGLKEAKDLV"
     gene            complement(319..825)
                     /gene="rplJ"
                     /locus_tag="VFX11_11930"
     CDS             complement(319..825)
                     /gene="rplJ"
                     /locus_tag="VFX11_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX4939377.1"
                     /translation="MTLRLEDKKEIVEAVNKAASGAFSAVVADYRGLTVGQMTALRVK
                     ARESNVYLRVVRNTLARRAFEGTNFAILSESLIGPTIIGLSLDENDMGAAARVFKDFA
                     KDNAKLEIKSGAYEGKLFTGAEIDVLARLPNREQALSMLANVLQAPVSKFGRLFTALK
                     EQKEQAAA"
     gene            complement(1072..1767)
                     /gene="rplA"
                     /locus_tag="VFX11_11935"
     CDS             complement(1072..1767)
                     /gene="rplA"
                     /locus_tag="VFX11_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX4939378.1"
                     /translation="MAKLSKRQAAIKAKLVPGKLYSIDEAVAFLTELPPAKFKESIDV
                     AVNLGVDPKKSDQVVRGATTLPAGTGKTVRVAVFAQGANAEAAKAAGADIVGFDDLAE
                     SIQAGNMDFDVVIATPDAMRVVGKLGTILGPRGLMPNPKVGTVTADAATAVKNAKAGQ
                     VRYRVDKAGIIHAGIGQIGFTADAIKQNVEALVADLKKAKPATSKGVYLQKITLSSTM
                     GPGLQIDLASITA"
     gene            complement(1770..2204)
                     /gene="rplK"
                     /locus_tag="VFX11_11940"
     CDS             complement(1770..2204)
                     /gene="rplK"
                     /locus_tag="VFX11_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX4939379.1"
                     /translation="MAKKIDAYIKLQVPAGKANPSPPIGPALGQKGVNIMEFCKAFNA
                     ATQKMEVGQPIPVVITVYSDRSFTFVMKTPPATFLLKKAAGLKSGSPRPNTQKVGKVT
                     RAQLEEIATLKKPDLTAADMDAAVRTIAGSARSMGLDVEGVN"
     gene            complement(2291..2824)
                     /gene="nusG"
                     /locus_tag="VFX11_11945"
     CDS             complement(2291..2824)
                     /gene="nusG"
                     /locus_tag="VFX11_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX4939380.1"
                     /translation="MSKRWYVVHAYSGYEKHVMRALTERVRLAGMEDSFGEILVPTEE
                     VVEMKNGVKRKSERKFFPGYVLVNMEMNDATWHLVKECPKVMGFIGGKADKPAPISEK
                     EANAILGRIQTNAESPRPKTLFEPGEVIRVTDGPFADFNGVVEEVNYEKSRMRVAVMI
                     FGRSTPVDLEFNQVEKT"
     gene            complement(2834..3202)
                     /gene="secE"
                     /locus_tag="VFX11_11950"
     CDS             complement(2834..3202)
                     /gene="secE"
                     /locus_tag="VFX11_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX4939381.1"
                     /translation="MSTELEASGSKLDAVKWLVVVVLLVAATLGNQYAAEASVWLRVG
                     GVVLACLVALLLAWTTSQGRSLVELSKKSWTEAQRIVWPSKDETWQTTLIVLAVVLVS
                     SLLLWGIDSLFGWGISAIIG"
     gene            complement(3256..3331)
                     /locus_tag="VFX11_11955"
     tRNA            complement(3256..3331)
                     /locus_tag="VFX11_11955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3296..3298),aa:Trp,seq:cca)
     gene            complement(3392..4582)
                     /gene="tuf"
                     /locus_tag="VFX11_11960"
     CDS             complement(3392..4582)
                     /gene="tuf"
                     /locus_tag="VFX11_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX4939382.1"
                     /translation="MAKAKFERNKPHVNVGTIGHVDHGKTTLTAAIATICAKTYGGEA
                     KGYDQIDSAPEEKARGITINTAHVEYDSPIRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRELLNQ
                     YKFPGDDTPIIRGSATEALSGNQGQYGEPAVLKLVETLDSYIPEPVRAIDRPFLMPIE
                     DVFSISGRGTVVTGRVERGIIKVGEEVEIVGLKDTTKTTCTGVEMFRKLLDEGRAGEN
                     CGVLLRGTKREEVQRGQVLAKPGTIKPHTQFEAEVYVLSKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGAIELPEGVEMVMPGDNIKMNVTLIAPIAMEEGLRFAIREGGRTVGAGVVA
                     KIIA"
BASE COUNT         1091 a         1473 c         1198 g         1014 t
ORIGIN      
        1 ccaccaggtc cttggcttcc ttgaggccca ggccggtagc ttcacgcaca accttgatca
       61 cgccaacctt gttggcgccg aaggaggcga ggataacgtc aaactcggtc ttttcttcag
      121 cggcggcagc accaccagca gcaggaccgg cagcaacagc aacagcggca gcggacacgc
      181 cgaacttctc ttcaaaagca gagatcagtt cgaccaggtc cattacgctc atttcagcaa
      241 ctgcattcag gatatcgtct ttggacagag ccattttcaa aactccaaaa tcttgtaaaa
      301 gggtgattca ggcggcaatc aggcagcagc ctgttccttc tgttccttga gcgccgtaaa
      361 gagacggccg aacttcgaaa caggcgcctg gagaacgttg gccagcatgg acaaggcctg
      421 ctcgcgattc ggcagacgtg caagcacgtc aatttccgca ccggtgaaca gcttgccttc
      481 ataggcaccg ctcttgattt ccagcttggc attgtccttt gcgaagtcct tgaacacacg
      541 ggcggcagca cccatgtcat tctcgtccaa cgacaggcca atgatggtcg ggccgatcag
      601 cgattcgctc agaatggcga aattggtccc ttcgaaagca cggcgtgcaa gcgtattacg
      661 gactacacgg aggtagacat tgctttcacg agctttcacg cgcagagcag tcatttgccc
      721 tacggtaaga ccacggtaat cggcgaccac agcagagaac gcgccggatg cggctttgtt
      781 aaccgcttcg acgatctctt tcttgtcctc tagtctcaga gtcactgtaa cctcctcgat
      841 tgattcgact ggcacaaagc cagccaccat gtctgctgcg gcaaacttcg ccgaagctcg
      901 cggggttaca gtctttgagg ctgaaccacc gcgtctgcgc aggctgagat tttaagctca
      961 ccccggatca ccgaggctcg cgcctgtggt ctttgacgct cgccgggcag gcccggcgac
     1021 ttcaaagtcc ttgttggcag gttttcctgc cgaaatcatc tcgcctaata cttaggcagt
     1081 gattgatgcc agatcaatct gcagcccagg acccatggtg ctggacagcg tgatcttctg
     1141 cagatacacg cccttcgacg tagcaggctt ggccttcttc agatcagcta ccagcgcctc
     1201 aacgttctgc ttgatagcat cagccgtgaa gccgatctgg ccaataccag cgtgaatgat
     1261 accagccttg tcaacacgat aacgcacctg gccagcctta gcgttcttga ccgcagttgc
     1321 agcatccgcc gtcacagtgc caaccttggg gttcggcatc aagccacgag gacccagaat
     1381 cgtaccgagc ttaccaacaa ctcgcatggc gtccggcgtc gcaatcacga catcgaaatc
     1441 catattgcca gcctgaatgc tctcggccag atcatcaaaa ccgacgatgt cagcacctgc
     1501 cgcttttgca gcctcagcat tggcgccctg ggcaaaaaca gcaacacgaa ccgtcttgcc
     1561 cgtaccggct ggcagagtgg tagcgccacg cacaacctga tcggacttct tgggatcgac
     1621 accgagattg acagcaacgt cgatcgactc cttgaacttg gccggaggca gttcggtcag
     1681 gaaggcaaca gcctcgtcga tgctgtacag cttgcccgga acgagcttgg ccttgatggc
     1741 ggcctgacgc ttggaaagct ttgccatgat cagttcactc cttccacatc aagccccatg
     1801 gaccttgcag agcctgcgat agtacgcaca gctgcatcca tatcagcagc cgtaaggtcc
     1861 ggcttcttca gggtagcaat ttcttccagc tgggcgcggg tcaccttgcc aaccttctgg
     1921 gtattggggc ggggagagcc gctcttcagg cccgcagcct tcttcaagag gaaagttgca
     1981 ggaggggtct tcatcacgaa agtgaaggag cgatccgagt aaaccgtgat caccacggga
     2041 atcggctgac caacctccat cttctgcgta gccgcattga aggccttgca gaattccatg
     2101 atgttaacgc ccttctgacc cagcgccggg ccaattggag gagaggggtt agccttgcct
     2161 gcaggaacct gcagcttgat gtaggcatca atcttctttg ccatttcatt actccgcggg
     2221 tgctagcgcc gtcaggctcc ccgtcaccaa gacagactac atctgccaga actgccgtag
     2281 cagcaaaaaa tcaggtcttt tccacctgat tgaattcaag atcaaccggc gtggagcgac
     2341 caaaaatcat caccgccaca cgcatacgac tcttctcgta gttgacttcc tcaaccacac
     2401 cattgaagtc ggcaaacggg ccatccgtca cacgaatcac ctcgcccggc tcaaacaacg
     2461 tcttcggacg tggagactct gcattggtct ggattcgccc aagaatggca ttggcctcct
     2521 tttcggaaat cggagctggc ttgtctgcct tccccccgat aaagcccatg acctttgggc
     2581 actcctttac caggtgccaa gtcgcatcgt tcatttccat attgaccaat acatagccag
     2641 gaaagaactt gcgctcgctc ttacgcttga cgccattctt catttccacc acctcttcgg
     2701 tgggaacaag aatttcgcca aaactatctt ccatgccagc cagcctgacg cgctcggtca
     2761 gcgcacgcat gacatgcttt tcatatccag agtaggcgtg taccacatac cagcgcttgc
     2821 tcatgccgcc cccttaacca atgatggccg aaatgcccca gccaaacagg gaatctattc
     2881 cccagagcag aagagaggaa acaaggacaa ccgcaagaac aatcagcgtg gtctgccatg
     2941 tttcatcctt gcttggccag acaatcctct gagcctctgt ccaagacttc ttggacaact
     3001 caacaagact acgcccctgc gacgtagtcc acgccagcag cagcgccacc agacatgcca
     3061 atacgacccc accaacgcgc agccatactg acgcctctgc agcatattga ttgcccagcg
     3121 ttgccgcgac aagcaacacc acgacaacca accacttcac tgcatccagc ttgcttccgg
     3181 aggcttccag ctctgtcgac ataaaccaca gcccgcagat atgacaaaaa cgcccttaag
     3241 cgttcctgtt aataatggca ggccaggagg gactcgaacc cccaacaccc ggttttggag
     3301 accggtgctc taccaattga actactggcc tgtatctatt cgacgacaag gcccggaatc
     3361 ttccgggccc gtcatcacac taaccctcgc ctcaggcgat gatcttggcg accacgccgg
     3421 cgccaacggt acggccgcct tcgcggatag cgaagcgcag accttcttcc atggcgatcg
     3481 gagcaatcag ggtcacattc atcttgatgt tgtcacccgg catcaccatc tcaacgcctt
     3541 ccggcagctc gatggcaccc gtcacgtccg tcgtacggaa gtaaaactgc ggacggtagc
     3601 ccttgaagaa cggagtatgg cgaccaccct cttccttgga cagcacgtac acttcagctt
     3661 cgaactgggt gtgcggcttg atcgtgcccg gcttggccag cacctggcca cgctgcactt
     3721 cttcacgctt cgtgccacgc agcagcacgc cacagttttc accagcacgg ccttcgtcca
     3781 gcagcttgcg gaacatttcc acgccggtgc aggtggtctt ggtggtgtcc ttcaggccca
     3841 caatttccac ttcttcaccg accttgatga tgccgcgctc aacacgacca gtcaccacgg
     3901 tgccacgacc ggagatggag aacacgtctt cgatcggcat caggaacgga cgatcaatgg
     3961 cacggaccgg ctcaggaatg tagctatcca gggtttccac caacttcaac acagccggct
     4021 caccgtactg accctggttg ccgctcaggg cttccgttgc ggaaccgcgg atgatcggag
     4081 tgtcgtcgcc cgggaacttg tactggttca ggagttcacg aacctccatt tccaccagct
     4141 ccagcagttc ttcgtcgtcg accatgtcgc acttgttcag gaacacgacg atgtagggaa
     4201 cacccacctg acgagacagc aggatgtgtt cgcgggtctg cggcatgggg ccgtcagcag
     4261 cggaacacac caggatcgca ccgtccatct gggcagcgcc ggtgatcatg ttcttcacat
     4321 agtcggcgtg accagggcag tccacgtgag catagtgacg gatcgggctg tcgtattcga
     4381 cgtgtgcagt attgatggtg atgccgcgag ccttttcttc cggagcagag tcgatctggt
     4441 catagccctt ggcttcgccg ccataggtct tggcgcagat ggttgcgata gcagccgtca
     4501 gcgtggtctt gccatggtca acgtgaccga tcgtgcccac gtttacgtgc ggcttgttac
     4561 gttcaaactt tgcctttgcc atcgtaactg ctcccttgcg gatgagttgc ccggtttcac
     4621 aaaacaaaag gcagccgcac gaggcgacca cccgttaaaa atatggagct cataaccgga
     4681 cttgaaccgg tgacctcttc cttaccaagg aagtgctcta ccgactgagc tatatgagaa
     4741 atacaccaaa agccattaaa aacaacaact tatgga
//
LOCUS       DASUEG010000635         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1141747, whole genome shotgun sequence.
ACCESSION   DASUEG010000635 DASUEG010000000
VERSION     DASUEG010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141747"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..915
                     /locus_tag="VFX11_11965"
     CDS             220..915
                     /locus_tag="VFX11_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4939383.1"
                     /translation="MEATKLRILFVEDHKALMDNLAEYFSGNGWEADFARDGLTALHL
                     LANHTYDVVVLDVMLPGVNGIGICERIRQDLKSDVPIILLTALDSIDDKITGFGAGAN
                     DYLTKPFDMRELELRIRAACRRQLQPQGTLRAGPLVFHPGTLTIVTDSGRSATLSGLS
                     ATLFEVLIRAYPNFVTYEDISNRVWGIETVEEHTIRTHIYLLRKQLKQALGQPLIKAL
                     YGRGYQLDVAQVT"
     gene            916..2166
                     /locus_tag="VFX11_11970"
     CDS             916..2166
                     /locus_tag="VFX11_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4939384.1"
                     /translation="MARSIAQRTFRLFLLVNLLLLLTTLLFAWWALEDLEETMLLSDR
                     QVELDYFRENGDRTQPQRTITAQLVSAFLPDGFDEADYLPIVFHGLPVPFQGEIEFLD
                     KEYFVVIQHFEGGNYYLAKDMALFEAREQTLTIYVLTLALVMGVASYVLATLFSRQIS
                     APVRRFALAISQLDEQRSDSRLQENFVDAELNEVAGAINHYLDRIESSLQREKTLINL
                     ASHELRTPVSVILGAARIIEKRQQLAADDARTLQRIIDAAEDMSANIRTLLALVRGSD
                     SLELEPCRIGEMLEQLHQDYALENPANAQRLHIARLTEEPVLQADKALARMLLHNLIS
                     NALHHTAGTVTITQHPTYVEVCDEGGPTGVPLATPNPKQASGLGLYIVHLACQQLGWQ
                     VEASGSAGGTRIRIDFAPLNPGPA"
     gene            complement(2147..>2785)
                     /locus_tag="VFX11_11975"
     CDS             complement(2147..>2785)
                     /locus_tag="VFX11_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939385.1"
                     /translation="VIRPSQLLRDHRFLFLAGWSLFTPLFFTLAGNILWTYVLPAQPA
                     MALLLAQLIWRRPLQQEAPSTAAPKMLAAGLQLWPAMLMPLGLCAMLVMAGMNPNLLK
                     SEKALVAFVQQQDAAASLVYAEHRPFSARYYSRGEAGLVELEQVPALRAVESHPMFLA
                     VPKSRRAQLQSLLGQSLQPVFENKGYLLLEIPAAKPVAAGGDEKQPLTPGPG"
BASE COUNT          567 a          920 c          809 g          489 t
ORIGIN      
        1 gtcgaccgtg actgcatcaa cgaacaggcg tctcacacct tcagccatga caacctgttc
       61 gacaccgtgc tgggactgct cagcatcgaa acccgcatct accgcccgca ggccgacatt
      121 ctcgcaccct gccagtcagc gcccatgatc tgagcctgct cctgcgtcca accctgcatg
      181 tatactgatc accatctcac gttgaggatg agttgtccga tggaagcaac caaactgcgg
      241 attctctttg tcgaggacca caaggccctg atggacaacc tggcggaata tttctcgggc
      301 aatggctggg aagcggattt cgcccgcgac gggctgaccg cgctgcacct gctggccaat
      361 cacacctacg acgtggtggt gctggacgtg atgctgcccg gcgtgaatgg catcggcatc
      421 tgcgaacgca tccgtcagga cctgaagtcc gacgtgccca tcatcctgct gaccgcgctg
      481 gattccatcg acgacaagat cacggggttc ggtgccggcg ccaacgatta cctgaccaaa
      541 ccattcgaca tgcgcgaact ggaactgcgc atccgcgccg cctgccggcg ccagttgcaa
      601 ccccagggca ctttgcgggc cggcccgctg gtatttcacc cgggcacact caccatcgtc
      661 accgacagcg gccgcagcgc caccctgtcc ggactcagcg ccaccctgtt cgaagtgctg
      721 atacgggcct atccgaattt cgtcacctat gaggacatca gcaaccgggt atggggcatc
      781 gaaacggtgg aggaacacac catccgcacc catatctatc tgctgcgcaa acagctgaaa
      841 caggccctcg gccagccgct gatcaaggcg ctgtacggcc gcggctatca actggatgtg
      901 gcgcaggtaa cctgaatggc ccgctccatt gcccagcgca ccttccgcct gttcctgctg
      961 gtcaacctgt tgctgctgct caccacgttg ctgttcgcct ggtgggcact ggaggatctg
     1021 gaggaaacca tgctgctgtc agaccggcag gtggaactgg attactttcg cgaaaatggc
     1081 gatcgcaccc agccccagcg caccatcacc gcccagctgg tgagtgcatt cctgccggac
     1141 ggcttcgacg aggccgacta cctgcccatc gtctttcacg gcctgccggt gccgttccag
     1201 ggggaaatcg aatttctgga caaggaatat ttcgtggtga tccagcattt cgagggcggc
     1261 aactattacc tggccaagga tatggccctg ttcgaggccc gcgagcagac tctgacgatc
     1321 tatgtgctga cactggccct ggtgatgggc gtggccagct acgtactggc cacgctgttc
     1381 agccggcaga tttcagcgcc ggtgcggcgc tttgccctgg caatatcgca gctggacgaa
     1441 cagcgttccg acagccgcct gcaggaaaat tttgtcgacg cggaactcaa tgaggtggcc
     1501 ggcgccatca accattatct ggaccggatc gaatccagcc tgcaacggga aaagaccctg
     1561 atcaacctgg cctcgcacga actgcgcaca ccggtttcgg tcattctggg cgctgcccgc
     1621 atcatcgaaa aacgccagca actggcggcg gacgatgccc gcacgctgca acgcatcatc
     1681 gatgcggccg aggatatgtc agccaatatc agaacactgc tggcactggt gcggggcagc
     1741 gattccctgg aactggaacc ctgccggatc ggcgaaatgc tggagcaact gcaccaggac
     1801 tacgccctgg aaaatcccgc caatgcgcag cggctgcaca tcgcccggct gacggaagag
     1861 ccggtgctgc aggcggacaa ggcgctggcc cgcatgctgc tgcacaacct gatcagcaac
     1921 gccctccatc acaccgcagg caccgtgacc atcacccagc atcccactta tgtggaagtg
     1981 tgcgacgaag gcggccccac tggcgtgccg ctggcgacac cgaacccgaa acaggcgtcc
     2041 gggctggggc tgtacatcgt gcacctggcc tgccagcaac tgggctggca ggtggaagca
     2101 tcggggtccg ccggcggcac ccgcatccgc atcgatttcg cgccgctcaa cccgggcccg
     2161 gcgtaagcgg ttgcttctca tcaccgccgg cggccaccgg tttcgcggca gggatttcca
     2221 gcaacagata acccttgttt tcgaacaccg gctgcagcga ctggcccagc agggactgca
     2281 actgagcgcg ccgggatttc ggcactgcca ggaacatcgg gtgcgattct accgcccgca
     2341 gcgccggcac ctgctcaagc tccaccagcc ccgcctcgcc acgggaataa taacgggcgg
     2401 aaaacggccg gtgttccgca taaacgaggg acgccgccgc atcctgttgc tgcacgaacg
     2461 ccaccagtgc tttttccgat ttcagcagat tgggattcat gcccgccatt accagcatcg
     2521 cgcacagccc cagcggcatc agcatggccg gccacagctg caacccggct gccaacattt
     2581 ttggtgcagc cgttgacggt gcctcctgct gcagcggccg gcgccagatc agttgcgcca
     2641 gcagcagggc catggccggc tgcgcgggca gtacgtaggt ccacagaatg ttgcccgcca
     2701 gggtgaaaaa cagcggggtg aacaggctcc atcctgccag gaaaagaaag cggtgatcgc
     2761 gcagcagttg cgacggccga atgac
//
LOCUS       DASUEG010000636         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1926496, whole genome shotgun sequence.
ACCESSION   DASUEG010000636 DASUEG010000000
VERSION     DASUEG010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926496"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFX11_11980"
     CDS             complement(<1..711)
                     /locus_tag="VFX11_11980"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008667 -
                     2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HEX4939386.1"
                     /translation="MEFYDKIVLVTGAAQGIGAAVAEHFSRAGARVTMLDCRPDITDC
                     ATQLARRTGGFALGLPVDVSDAAAVASAVQTVEMDMGPIDILVNVAGILRMGPLLDMS
                     TTDWSRTFAINCDGVFNLSTAVARNMVQRRRGCIITVGSNAAATPRMNMGAYAASKAA
                     THQFTRCLGLELAPFGIRCNIVSPGSTDTPMQQQLWTTPNGRASVLEGSLEKFRLGIP
                     LQRIATPDDIAAAVAFLAS"
     gene            complement(711..>2076)
                     /locus_tag="VFX11_11985"
     CDS             complement(711..>2076)
                     /locus_tag="VFX11_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX4939387.1"
                     /translation="DDELKLSGQRVSPAEIESALSRCPGISDGVALVVDSVTGAKQIV
                     ACVIPAADAAPDMAQLRRWLAQYLLPAAVPRHFLFVTQFPKNANGKIDRRQLREQWYT
                     QGSADTTLITDATSELERTIIHVWRDILGSVQIDRHSDFFALGGKSLQAIQVINRLSR
                     QLQRELAVSALFRHPSVSALAHYLENTATSICEPSAPRTQDLAPLIQLQSGQGNALFC
                     VHPADGVSWCYLGLLRHLPTIPIYGLQAEGLTGALPADFDTMIERYCERIFAQQAQGP
                     YQLLGWSSGGGIAHAIAARIQQEGHTVALLAMMDAYPAGCWTDAPAPTRRDAIRMLLD
                     DSKNAFDADGNVLGETQLLAQVLASDHALAALGETQLNRMIDTTLHGMQLYRRARHPH
                     FNGDLLFFNAARRLPGQPEWTAWRDFIGGHIQVVNIDSDHFAMHLPPAAAQIGAVLAA
                     KVSS"
BASE COUNT          391 a          608 c          680 g          397 t
ORIGIN      
        1 gctggcgagg aaagcgacgg cggcggcaat gtcgtctggc gtggcaatgc gctgcagcgg
       61 aatacccagg cgaaattttt ccagtgagcc ttccagtacc gatgcacgac cgttgggtgt
      121 cgtccacaat tgttgctgca tcggcgtgtc ggtggagccg ggcgatacga tattgcagcg
      181 aatgccgaac ggcgccagtt ccaggccgag gcagcgggtg aactggtgag tggcggcttt
      241 tgaagcggcg taagcgccca tattcatgcg cggcgtggcg gcggcgttcg atcccaccgt
      301 gatgatgcag ccgcgccgcc gttgcaccat gttgcgcgcg acagccgtgc tgagattgaa
      361 cacgccgtcg cagtttatgg cgaaggtgcg cgaccagtcc gtcgtgctca tgtccagcaa
      421 cggtcccatg cgcagaatgc ccgccacgtt gaccagaata tcgatgggcc ccatgtccat
      481 ttccacggtt tgtactgcac ttgcgacggc cgcggcatcg ctgacatcca ctggcaatcc
      541 cagtgcaaag cctccagtgc ggcgggccag ttgtgtggca cagtcagtga tatcaggccg
      601 gcaatcgagc atcgtgacgc gggcgccggc gcgggaaaag tgctctgcca cagcggcgcc
      661 aatgccctgc gcggcgccgg tcaccagcac gattttgtca tagaattcca ctagctgctt
      721 accttggccg ccagtacggc gccgatctgc gcagcggcgg gcggcaggtg catggcgaaa
      781 tgatcgctgt cgatattcac cacctggatg tgcccgccaa tgaaatcccg ccacgcggtc
      841 cattccggct gtccaggcag gcggcgtgcc gcgttgaaaa acagcaggtc gccgttgaag
      901 tggggatgtc gcgcgcgccg gtaaagctgc atgccatgca atgtggtgtc gatcatgcga
      961 ttgagctgcg tttcgcccag tgccgcaagc gcgtgatcgc tggcgagaac ctgcgccagc
     1021 agttgagtct caccgagaac attgccatcg gcgtcaaagg cgtttttgct gtcatccagc
     1081 aacatgcgga tggcatcgcg gcgagtgggc gcgggcgcgt ccgtccagca gccggccggg
     1141 taggcgtcca tcatggccag cagcgcaacg gtgtgccctt cctgttgtat ccgtgctgca
     1201 atggcgtggg cgatgccgcc gccggaggac cagcccagta attgatatgg gccctgcgct
     1261 tgttgcgcga atatgcgttc gcaatagcgc tcgatcatgg tgtcgaaatc cgcaggcagc
     1321 gcgccggtca atccctctgc ctgcaaaccg taaataggga tggtcggcag atgccgcagc
     1381 aatcccaggt aacaccagct cacgccgtcg gccggatgca cgcagaacag ggcgttgccc
     1441 tggccggatt gcagctgaat cagcggcgca agatcctgcg tgcggggagc ggatggttcg
     1501 caaatgctgg tggcggtgtt ttccagataa tgtgccagcg cgctgaccga tggatgccga
     1561 aacagtgccg acaccgccag ttcccgttgc agctgtcggc tcagccggtt gatcacctga
     1621 atggcctgca gggattttcc gccgagggca aaaaaatcgc tgtggcggtc gatctgcacg
     1681 ctgccgagga tatcgcgcca gacgtgaatg atggtgcgtt caagttccga agtggcgtcg
     1741 gtgatgaggg tggtatcagc cgagccttgt gtgtaccact gttcgcgcaa ctggcgccgg
     1801 tcgattttgc cgttggcatt tttcgggaac tgcgtaacaa acagaaaatg acggggcacg
     1861 gcagcgggta gcagatactg tgccagccag cggcgcagct gagccatatc cggagctgca
     1921 tctgctgccg gaatgacaca ggccacaatc tgcttggcgc cggtaacact gtccaccacc
     1981 agcgccacac cgtcgctgat gccggggcaa cgggacaggg ccgattcgat ttcggcgggc
     2041 gacacgcgct ggccgctgag tttgagttcg tcgtcc
//
LOCUS       DASUEG010000637         7065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1461189, whole genome shotgun sequence.
ACCESSION   DASUEG010000637 DASUEG010000000
VERSION     DASUEG010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461189"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2888
                     /locus_tag="VFX11_11990"
     CDS             <1..2888
                     /locus_tag="VFX11_11990"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HEX4939388.1"
                     /translation="GFEDFVTGAPYNTASAGVAYIVYGQAGATRSNVNTSAINNGTNG
                     FRITGQSASDYLGASVGGAGDINDDGYDDIYVIASRDDDYNTDAGILYVIYGKASNTN
                     VAISSFSSSVGFRVTTAELGAAMGDAFTGYATNGQAIDAGGDFNGDGVQDLVVGLRFS
                     DENGADSGKAYVIFGRAGATRTDVNLDLIDPTGTDGVMIYGASAGAQLGQAVQFVGDY
                     DADGYDDVVASAIFSDAVANDGGQAFLIFGSPGPVFSAVDVSALSGASGFKISTSELS
                     GVLGHGIGAGDFNGDGISDLLISENGYDATVGAAHVIYGDAGGVYSNIAVDTMLSSTG
                     YNIFGQGVGEYLGHSLSGAGDFNADGIDDLLVASYLRDDGGADSGAAWLITGALGNAR
                     ADLNIGSITAADGFKISGGGIGDHFGQSTAYVDANGDGFSDLILGATWGDNASIDGGE
                     TLVLWGREFATAVTSGLTGTGGADNLVGTSGNDTIVSGGGADAISAGAGNDTIQLSDT
                     GFFNIEGGEGTDILQFTTGPNTLDLTAYGPETINGIEVIDLGSTSTSLIVSKESVQDM
                     SRESRALFVRGSLGSVTSTTGDAWCYTGTKVEGGITYHVYLDNGAILYIQSGLTHSGV
                     GNFNSTQQYTFNTTVGGANVSSAVTDFPVLIRVSTTIRNTLQASRADLRFTDKDQVTW
                     LPYEIEIGASGELYAWVLVPQVDGNSSSDYIVMHYNDKVDGSVADRQDPTRVWVDYAG
                     VWHFDEASGNALDSTAYQNNGTQSGTVSRESSFVGRGVRFNTSTERFTVPYDLSFNAA
                     SKDFTVELWLDGTTNCTTDPFVSKDYLTGVSRGMGTSRNWRVMTGGAFPWWAGGFVGS
                     PGFQIEDTGGSDSLTSAGWLSTDCSNHMVVEHSRTAGSKVYISGALAHSDSSVRNVEN
                     LQDFIIGNSGGSAEIRIDEVRLMTKATSAARVKLIYQNQSGTSLVSP"
     gene            complement(2911..4824)
                     /locus_tag="VFX11_11995"
     CDS             complement(2911..4824)
                     /locus_tag="VFX11_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4939389.1"
                     /translation="MFDMSLEDLMQVEVDTASRRPSTWREQPGVITVFSADDMVAMGA
                     RTLLDVVQRIPGTSLGIDGRNSIGLMMRGSWTLEGKILVLLNDMPLNELLYGSWSPLP
                     YIPVGQLDRVEVLRGPGSTQYGGSAQLAVIRIFTRQPTGGNDGQLDYTAIGQSGEPTQ
                     MLGLSQRFASGEMQGALNISANRGVWGNETWVDNLGQEVSTADADTDGSTLALTADLG
                     RRDHLQLYHEQFDINAIQGFGTAEPQGMVVLNRSLLSYSHDWKPGATLTLSPRLAYRH
                     DNLRVRAPEEPTVFDMTSQSMGGGLDLKWNYDAHNLLSAGFEYLLETAEANSVSGPFF
                     PQPTELYFGNSSDQDYERSSVYGDWNLQLGDYVLILGARASHHQYAGNAVTPRAGLTR
                     ASDNWHFKWLYGEAFREPNIETINYGTSTEYISPERTVAHEIELGHRLLDNGYLTLSL
                     FDQRIRDPIIFNQDRIDEFTYNNNPDMYSQGAELQYLYRAEGFSLQANYSWTHSNDDD
                     LQVYDVPQQSGQFIGAPAHVGNAWLSLSTPVAHLSAMLGARYVGSRQALDFDPVLAQT
                     QPDFPLGMQRLDAEFTVNAALRYEIRATTLTLGLDNLFDEEQYMPQPYSGFSTPFPYG
                     SRSIWLRGEWEWP"
     gene            complement(5205..5924)
                     /locus_tag="VFX11_12000"
     CDS             complement(5205..5924)
                     /locus_tag="VFX11_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939390.1"
                     /translation="MSGPDREGSRMKTQTSTDRSNVIDLFTRRPIDEVVDRVVRLAPE
                     LDGLEMLYTNDTSPDRLYSLKVVCWALRESGEVVGMVPWLNQIVPCTEINDPLNGHWE
                     GYRDPGTNEIFYTAPRHKIMELELAEEYYHLDHVKEGQTVQEIPDSIGTHAVLTDNGF
                     RSFILIEVLSWRLLADGTVHAMLVDEEKVKSTPVLPGDPCLYPAQEKKEFKYFFQHRI
                     ANKIKEQDPEALAAISMLIEN"
     gene            complement(6094..>7065)
                     /locus_tag="VFX11_12005"
     CDS             complement(6094..>7065)
                     /locus_tag="VFX11_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4939391.1"
                     /translation="GYLEGVSWGMPAITLFLALRCLTEGMSFTRPVMAVSLIGLCANI
                     PANYVLMYGKLGLPAMGGVGCGWATGFVMWLMLAIMLVHCLLSANYRATGWFRQFAGP
                     QPTLLLPLVKLGLPIGLAIFIEVSLFCVIALFIASIGTQMVAGHQIALNVASMVFMIP
                     LSLSLALTVRVGFNLGRGNLPGVHHALRGGMALIILLALVNSTLMVVFREQIAGIYTD
                     DADVIALATSLLVFAAVFQLSDGLQVGANGVLRGLHDTTVPMLLTVVAYWLLGLPIGY
                     ALGMTDWLLPVPLGAQGFWVGLVAGLTAAAILLNIRVRLRLRALAQT"
BASE COUNT         1399 a         2112 c         2198 g         1356 t
ORIGIN      
        1 acggcttcga ggatttcgtc accggcgcgc catacaacac tgccagcgcc ggcgttgctt
       61 atattgtcta cggtcaggcg ggcgccaccc gcagcaacgt caacacgtcc gccatcaaca
      121 acggcaccaa tggcttcagg atcacggggc aatccgcgtc cgattatctg ggtgcctcgg
      181 tgggcggtgc cggtgacatc aacgatgatg gatacgacga catttatgtg attgcatccc
      241 gcgacgacga ctacaacacc gatgccggca ttttgtatgt catctacggc aaggccagca
      301 ataccaacgt ggcgatcagc agtttcagca gcagcgtcgg attccgggtt accacggcgg
      361 agctgggcgc ggccatgggt gatgccttca caggctatgc caccaacggt caggctatcg
      421 atgccggcgg cgatttcaac ggcgatggcg tgcaggatct ggtggtcggt ctccgcttca
      481 gcgacgagaa cggcgctgac agcggcaagg cctatgtcat cttcggccgc gctggcgcca
      541 cccgtaccga tgtgaatctg gacctgatcg atcccaccgg cacggacggc gtgatgattt
      601 acggcgccag cgcgggcgca caactggggc aggcggtgca attcgtcggt gactacgatg
      661 ccgatggtta tgacgacgtc gtggccagcg ccattttctc cgatgcagtg gccaatgacg
      721 gcggtcaggc cttcctgatt ttcggatcgc ccggccctgt gttcagcgca gtcgatgtgt
      781 ccgcactgtc cggtgccagc gggttcaaga tttccacgtc ggaactgtcc ggcgtgctgg
      841 gccatggaat tggtgccggt gatttcaatg gcgacggtat cagcgacctg ctcatttccg
      901 agaacggcta cgacgccacc gtgggggccg cccatgtgat ttacggcgat gccggcggtg
      961 tgtattccaa tatcgcagtg gacaccatgc tcagcagtac cggctacaac attttcggcc
     1021 agggtgtggg cgagtatctg gggcacagcc tgtctggcgc cggcgacttc aatgctgatg
     1081 gcatcgacga tctgctggtg gcctcctatc tgcgcgatga cggtggtgca gattccggcg
     1141 ccgcctggct gattaccggt gcgttgggca atgcccgtgc ggacctgaat atcggcagca
     1201 ttacggcagc ggacggtttc aagatttccg gcggtggcat cggcgatcat ttcggccagt
     1261 ccacggctta tgtcgatgcc aacggtgacg gcttcagcga tctgatcctg ggcgccacct
     1321 ggggcgacaa cgcgtcgatc gatggcggcg aaacgctggt actgtggggg cgtgaattcg
     1381 ccaccgccgt caccagcggc ctcaccggca ccggcggcgc cgacaatctg gtgggtacca
     1441 gcggcaacga caccatcgtc agtggcggcg gtgccgacgc gatctcggcc ggcgccggca
     1501 acgatacgat ccagctcagc gacaccggct tcttcaacat cgaaggcggc gagggcaccg
     1561 acatcctgca gttcaccacc ggtcccaaca cgctggattt gacggcctac ggaccggaaa
     1621 ccatcaatgg catcgaagtg atcgatctgg gcagcaccag taccagcctg atcgtaagca
     1681 aggaatcggt gcaggatatg tcgcgcgaaa gccgtgcgct gtttgtgcgc ggcagcctgg
     1741 gcagcgtcac gtccaccacc ggcgacgcct ggtgttacac cggcaccaaa gtcgagggcg
     1801 gcatcaccta ccatgtctat ctcgacaacg gtgcgattct ctacattcag agcggcctca
     1861 ctcacagcgg cgtgggcaat ttcaattcca ctcagcagta cacgttcaac accacagtcg
     1921 gtggcgccaa tgtgtccagc gcggtgacgg atttcccggt gctgattcgc gtatccacca
     1981 ctatccgcaa cacgctgcag gccagccgcg ccgacctgcg tttcaccgac aaggatcagg
     2041 tgacctggct gccttatgaa atcgaaatcg gcgcgtccgg cgaattgtat gcctgggtgc
     2101 tggtgccgca ggtggatggc aacagcagca gcgattacat cgtgatgcac tacaacgaca
     2161 aggtggatgg atcggtggca gaccggcagg acccgacgcg ggtgtgggtg gactacgccg
     2221 gcgtgtggca cttcgatgaa gcctcgggca atgcgctgga ttccaccgcc taccagaaca
     2281 acggcaccca gagcggaacc gtatcgcgcg aaagcagctt tgtcggccgg ggcgtgcgct
     2341 tcaatacaag cacggagcgt ttcaccgtgc cctacgatct ctcgttcaat gctgccagca
     2401 aggatttcac agtggaactc tggctggacg gcaccaccaa ctgcaccacc gatccgttcg
     2461 tgtccaagga ttatctgact ggcgtgagcc ggggtatggg aacatcacgc aactggcgtg
     2521 tcatgaccgg cggtgccttc ccctggtggg cgggtggatt cgtcggcagc cccggcttcc
     2581 agatcgagga cactggcggc tccgattctc tgacctccgc aggctggctg agcaccgact
     2641 gctcgaatca catggtggtg gagcacagcc gtaccgcagg ttcgaaggtg tatatcagcg
     2701 gcgcgctggc gcacagtgac agctcggtgc gcaacgtgga aaacctgcag gatttcatca
     2761 tcggcaattc cggcggcagt gcggagatac gcattgacga agtgcgcctg atgacaaaag
     2821 ccaccagcgc cgcccgcgtc aagctgattt accagaacca gtccggcacg tcgctggtat
     2881 cgccctgata gccggcgtca gtgcagctgt ttatggccat tcccattccc cccgcaacca
     2941 gatgctgcgg ctgccatacg gaaacggcgt gctgaaaccg gaatagggtt gtggcatgta
     3001 ctgttcttcg tcgaacagat tgtccaggcc cagggtgagc gtcgttgccc ggatctcata
     3061 gcgcagtgcc gcattcacgg tgaattccgc gtccaggcgt tgcatgccca gcggaaaatc
     3121 aggctgcgtt tgtgccagca ccggatcaaa atccagcgcc tggcgactgc ccacatagcg
     3181 cgcgcccaac atcgcggaga gatgagccac gggtgtcgac aggctgagcc aggcattgcc
     3241 aacatgcgca ggcgcgccga taaactgccc cgattgctgc ggcacgtcat agacctgcag
     3301 atcatcgtcg ttcgaatggg tccagctgta attggcctgt agcgagaagc cttctgcccg
     3361 atacagatac tgcaactcgg cgccctggct atacatgtcc gggttgttgt tgtaggtgaa
     3421 ctcgtcaatc cggtcctgat tgaaaatgat cggatcgcga atgcgctgat cgaacagcga
     3481 cagcgtcaga tagccgttgt ccagcaggcg gtgacccagc tcgatttcat gtgccacggt
     3541 tcgttccgga ctgatgtatt cggtcgaggt accgtaattg atggtctcga tattgggttc
     3601 gcgaaaggct tctccgtaca gccacttgaa atgccagttg tcactggcgc gggtgagccc
     3661 cgcccgtggc gtgaccgcat tgcccgcgta ctggtggtgg ctggcccgtg ctcccaggat
     3721 cagtacgtaa tcgcccagtt gcaggttcca gtccccgtaa accgagctgc gctcgtaatc
     3781 ctgatcgctc gaattgccaa agtagagttc agtgggctgc gggaaaaaag gtccgctgac
     3841 gctattggct tcggcggttt ccagcagata ttcgaaacct gcactcagca ggttgtgagc
     3901 gtcgtagttc catttcaggt ccagtccgcc gcccatgctt tgggaggtca tgtcaaatac
     3961 ggtgggctcc tcgggagcgc gcacccgcag gttgtcatgc cggtaggcaa ggcgcgggct
     4021 gagggtcagc gttgcgcccg gtttccaatc gtgggaataa ctcagcaacg agcggttcaa
     4081 caccaccatg ccctggggct ccgccgtgcc aaagccctgg atggcgttga tatcaaactg
     4141 ttcgtgatac agctgcaggt gatcgcggcg gcccagatcg gcggtgagtg ccagagtgct
     4201 gccatccgtg tccgcgtcgg cggtgctcac ttcctggccc aggttgtcga cccaggtttc
     4261 attgccccac acgccgcggt tggcgctgat attcagcgcc ccctgcatct cgccactggc
     4321 aaagcgctgg cttaatccca gcatctgggt aggctcaccg gactgcccga tcgccgtgta
     4381 atccagctgg ccgtcgttcc cgccggtggg ctgacgggtg aagatgcgaa tgaccgccag
     4441 ctgcgcgctg ccgccgtact gggtactgcc gggcccgcgc aacacttcca cccggtccag
     4501 ctgacccacg gggatatagg gcaggggcga ccagctgcca tagagcagct cgttcagcgg
     4561 catgtcgttc agcagtacca gaatcttgcc ttccagtgtc cagctgccgc gcatcatcag
     4621 gccgatcgag ttgcgtccgt cgatgccgag gctggtgccg ggaatgcgct gcaccacatc
     4681 cagcagggtt ctggcaccca ttgccaccat gtcatccgcc gagaacacgg tgatcacgcc
     4741 cggctgttca cgccaggtac tggggcgccg gctggcggta tcgacctcca cctgcatcag
     4801 gtcttccagc gacatgtcaa acatgctctg ctgctggcgg tcgtgtcggg cctggcctgg
     4861 ttccgccaaa agcaaaatga atacgcccac tgcataaggc aacaggaaga atgagtgcct
     4921 gaagagtggc gtggtcataa caccagatcc tcgacgaaaa ggaatggtca caccggatat
     4981 agattggaaa cggtctgcac gagccagagt aaaaactgtc gtacaacggt cctgaggaag
     5041 gattcaacga acgactctga taaccttaaa gactagctgg tttttcgcgc gccgctattg
     5101 aattgcgttg ccgcaggaag ttggattcag cggcgaactg cggggaaaaa tattcctgaa
     5161 gtgttgcacc ccaacgtgcg tacgcagtga agtctggaat ggcttcagtt ttcgatcagc
     5221 atcgaaatcg ccgccagggc ttccgggtcc tgttccttga tcttgttggc gatgcggtgc
     5281 tggaagaaat atttgaattc ctttttctcc tgcgccggat acaggcaggg atcgccgggc
     5341 agtaccgggg tggatttcac tttttcctcg tccaccagca tggcgtgcac ggtaccatcc
     5401 gccagcaggc gccagctcag cacttcgatc aggatgaacg agcggaaacc gttgtcggtc
     5461 agcaccgcgt gggtgccgat ggaatccgga atttcctgca cggtctggcc ttccttcacg
     5521 tgatccaggt ggtagtactc ttccgccagt tccagttcca tgatcttgtg gcgcggcgcg
     5581 gtgtagaaaa tctcgttggt gcccgggtcg cggtaacctt cccaatgacc gttgagggga
     5641 tcgttgattt cagtgcaggg gacgatctgg ttgagccagg gcaccatgcc caccacttcg
     5701 ccggattccc gcagcgccca gcagacgact ttcagggagt agaggcggtc gggggaggtg
     5761 tcgttggtgt agagcatttc cagtccatcc agctcggggg cgagccggac gacgcggtcc
     5821 accacttcgt cgatggggcg tctggtgaag aggtcgatca cattgctgcg atcagtggac
     5881 gtctgcgttt tcatgcgact accctcgcgg tccgggccgg acatcccgtg aatcctggtg
     5941 atgtgcagca ccggtcatca tggacttact gactcaagat taatctaagt atagtcggcc
     6001 cgaaaatgcc aattggcact aaggtgggcg gtcaaaaggc cggttcgggg cggtcattga
     6061 cggggaatgg tgacggtaaa ccggcgaagg aagttaggtt tgtgccagcg cccgcagccg
     6121 caggcgcacc cggatattga gcaggatcgc cgccgccgtc aggcccgcca ccagtcccac
     6181 ccagaagccc tgcgccccca gcggcaccgg cagtagccag tcggtcatgc ccagggcata
     6241 gccgataggc aggcccagca gccagtaggc caccacggtc agcagcatgg gcacggtggt
     6301 gtcgtgcagg ccccgcagca cgccgttggc acccacctgc aggccgtcgg acagctggaa
     6361 caccgccgcg aacaccagca gcgaggtggc cagggcgatc acgtcggcgt cgtcggtgta
     6421 gatgccggca atctgttcgc gaaataccac catcagggta ctgttcacca gcgccagcag
     6481 gatgatcagc gccataccgc cgcgcagcgc atggtgcacg ccgggcaggt tgccccggcc
     6541 caggttgaag cccacccgca cggtgagcgc cagggacagg ctgagcggaa tcatgaacac
     6601 catggatgcc acgttgaggg cgatctggtg cccggccacc atctgggtgc cgatggaggc
     6661 gatgaacagc gcgatgacgc agaacaggct gacttcgatg aagatcgcca acccaatggg
     6721 cagccccagt ttcaccagcg gcagcagcag ggtgggttgc ggcccggcaa actggcggaa
     6781 ccagccggtg gcgcggtagt tggcggacag caggcagtgc accagcatga tggccagcat
     6841 cagccacatc acgaacccgg tggcccagcc gcagccgacg ccgcccatgg ccggcaggcc
     6901 gagcttgccg tacatcagca catagttggc ggggatgttg gcgcacaggc cgatcaggct
     6961 gaccgccatg accgggcggg tgaaggacat gccttcggtc agacagcgca gggccaggaa
     7021 cagggtgatg gccggcatgc cccaggacac cccttccaga taacc
//
LOCUS       DASUEG010000638         5712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1191894, whole genome shotgun sequence.
ACCESSION   DASUEG010000638 DASUEG010000000
VERSION     DASUEG010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191894"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VFX11_12010"
     CDS             <1..1397
                     /locus_tag="VFX11_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX4939392.1"
                     /translation="SRELMTRGEIKEVDFLSYWAAAWIQFMVHDWMAHRNSAGDHHSI
                     PLQGDDPLADELGSDMPVAKTDIDPAWTPYENTPPAYVNRNTHWWDGSQLYGSDEETA
                     RKVRTLSGGQLKLEASGRLPLDPQTGLPLTGFSENWWLGLQMLHELFVKEHNAIAVML
                     QQKHPGWDDELLYNKARMINAAVMAKIHTVDWTPSILNTPTLKTAMNADWEFVVGGGK
                     NLRGTPYSLTEEFVAVYRMHPLIRDEVAIRSYQAGEVLQPLPTTEQAFAKAAGVMDRF
                     GTANVFYSFGVTHPGQITTNNYPKFLQELRLPDGTLMDLAAVDILRDRERGVPRYNEF
                     RRQLHMPPIRDFTDLTPDRELAEKLRAIYHNDVEAIDTLVGQLAEGYRPPGFGFSDTT
                     FRIFTMMAPRRFQADRFYTSDFNKAVYTAEGMTWIRNANMKSVILRHVPELKTVMAKV
                     GNPFFPWTTDSSKW"
     gene            complement(1462..2361)
                     /locus_tag="VFX11_12015"
     CDS             complement(1462..2361)
                     /locus_tag="VFX11_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4939393.1"
                     /translation="MTKSAAQPVARSTRSHTTPIQTRWEPRTAQELDDAIFGCLDNVG
                     LLAGVANTIMQLAHPAVGYGVIESRVTSGSLLHHPFKRTRTTLTYLGVALQGSTEEKL
                     AYRKAISHAHAQVTSTATSPVQYRALDPELQLWVAACLFWGYVDTREKFHGPMTPEQL
                     AEFYEYAKPLGTTLQVRPDMWPKDLEAFWEYWNRQIDSIQIDDTVRGWLTRLMDLSFM
                     GPFISIPLGNFAKLMTGGFLHPKIRAQMQIEWTPEKEQQFRKVTTAIGRVNRRLPRSL
                     RQLPTTLLLWDFRRRLKKGLPLN"
     gene            2441..3097
                     /locus_tag="VFX11_12020"
     CDS             2441..3097
                     /locus_tag="VFX11_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939394.1"
                     /translation="MGLVRREWVRQQQAIDLKQKKVPRQVRAQATLQAILQASAQVLI
                     QEGYAGLTTNAVAERAGVSIGSVYEYFPGKEAIVAVLTTRAVADTLREIEDSLNGADP
                     ADLRAAMNQFVRSLYRITCERRDLLKILLFQVPFVHEIPAMQSLRAELIRLVLLGAGR
                     TRHEHQIEARPETLYLIGSMTAAALLQLAITPPPGLDVNRILDELAEKVVDWLVERKS
                     "
     gene            complement(3094..4758)
                     /gene="panP"
                     /locus_tag="VFX11_12025"
     CDS             complement(3094..4758)
                     /gene="panP"
                     /locus_tag="VFX11_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative pyridoxal-dependent aspartate
                     1-decarboxylase"
                     /protein_id="HEX4939395.1"
                     /translation="MTDKPRYAEASMEAMYRIFTVPEAPDSTLGKIDQKLSQNLAGFL
                     SEHIVAVEKDLKELEKDFQDSQIPQKPIFVSDQADFLMEKLVAQSVHTASPSFIGHMT
                     SALPYFMLPLSRIMMALNQNLVKIETSKAFTPMERQVLGMVHRLIYEQDDAFYAKWMH
                     LPGHSLGSFCSGGTIANTTALWIARNHKFGPNGKFRGIQREGLHGALKHYGYNGAAVL
                     VSERGHYSLGKAADVLGIGRDFMITIKTDNKNKARIPELKEQIKRLQGENVAIFAIVG
                     VAGTSETGSVDRLDEMADIAQEIGVHFHVDAAWGGPTLFSKKYRHILKGVERADSVTI
                     DAHKQMYVPMGAGMVLFRDPTLVSAIEHHADYIIRKGSKDLGSYTLEGSRPGMAMLVQ
                     SGLKIIGHDGYEILMDQGIEKAKLFADMIKADNEFELITEPELNILTYRYLPDFVRAK
                     LAVATPAEVDAIQEVMNRLTRSIQKTERAHGKSFVSRTTLMPEEYDRRRTVVFRVVLA
                     NPLTNHQILQDVLTEQKQIASGPDCASLMAELQALCVPPARKVQAG"
     gene            5137..5442
                     /locus_tag="VFX11_12030"
     CDS             5137..5442
                     /locus_tag="VFX11_12030"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEX4939396.1"
                     /translation="MNRLCRGALGAALILLPLITQARVEPREGEEPIEATEPKITIVE
                     DRQETLQIYQFNGHVYGIRVIPKNGKPYNLVDTNGDGKFIQNAADRILVPEWVLVKF"
     gene            5463..>5712
                     /locus_tag="VFX11_12035"
     CDS             5463..>5712
                     /locus_tag="VFX11_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4939397.1"
                     /translation="MAVYTQLSENEVSAFLQGFGLVRLTRLRGTEAGIENTNYFVDAE
                     DAGGRAHTLVLTLFERGADALPYFVALTTYLAEQGLPVP"
BASE COUNT         1165 a         1597 c         1753 g         1197 t
ORIGIN      
        1 tcagccgcga actgatgacc cgtggcgaga tcaaggaagt ggattttctc agttactggg
       61 cggcggcctg gatccagttc atggtgcatg actggatggc acaccggaat tcagccggtg
      121 accatcacag cattccgttg cagggcgatg atccgctggc ggatgagctc ggttccgaca
      181 tgccggtcgc gaaaacggat atcgatccgg cctggacacc ctacgagaac acgccaccgg
      241 cctacgtcaa ccgcaacacc cattggtggg atggatcgca actgtatggc agcgacgagg
      301 agacggcgcg caaagtccgc accttgagtg gcggccagct gaaactggag gcaagcggcc
      361 gtctgccgct ggacccgcaa accggtctgc cactcaccgg attcagcgag aactggtggc
      421 tgggcctgca gatgctgcat gagctgttcg tcaaggaaca caacgccatc gcggtcatgc
      481 tgcaacagaa acacccgggc tgggacgacg agctactcta caacaaggcg cgcatgatca
      541 acgccgctgt catggcaaag attcacaccg tggactggac accgtcgatt ctcaataccc
      601 cgacgctcaa gactgcaatg aatgccgatt gggaatttgt tgtgggtggc ggcaagaatc
      661 tgcgtggcac accgtacagc ctcacggagg aatttgttgc ggtataccgc atgcatccgc
      721 tgatccggga cgaagtggcg atccgcagtt accaggcagg tgaagtgctg cagccgctgc
      781 ccaccaccga gcaggcgttt gccaaggcgg cgggtgtgat ggatcggttt ggcactgcca
      841 acgtgttcta ttcctttggc gtcactcatc cgggccagat caccaccaac aactatccaa
      901 aattcctgca ggaactgcgc ctgccggacg gcaccttgat ggatctggcg gcggtggaca
      961 tattgcggga tcgcgagcgc ggtgtaccgc ggtacaacga gttccggcgc cagctgcaca
     1021 tgccaccgat ccgggatttc accgacctga caccggacag ggaactggcg gagaaattgc
     1081 gtgcgatcta tcacaatgat gtggaagcca tcgatacgct ggtaggccag ctggcggaag
     1141 gctatcggcc accgggcttc ggtttcagtg ataccacttt ccgcattttc accatgatgg
     1201 caccgcgccg gttccaggcc gaccgcttct acaccagcga tttcaacaaa gcggtttaca
     1261 ctgccgaggg catgacctgg atacgcaatg cgaatatgaa gagcgtgatc ctgcgtcacg
     1321 tgccggaact gaaaaccgtc atggccaagg tcggcaatcc gttctttccc tggacaaccg
     1381 acagcagcaa atggtgatgg aacaataaaa cgcgacgtat gcgcaggcag cgggttccag
     1441 gctgcctgcg cctcgcagca ctcagttcag tggcaggcct ttcttcaggc ggcggcggaa
     1501 atcccacagc aacagcgtgg tgggtaactg ccgcaggctg cggggcaggc ggcggttgac
     1561 gcgaccgatc gcagtggtga ccttgcggaa ttgctgctcc ttttcgggtg tccattcaat
     1621 ctgcatctgc gcgcggattt ttggatgcag aaagccgccg gtcatcagct tggcgaagtt
     1681 gcccagcggg atgctgatga acgggcccat gaaactcagg tccatcagcc tggtgagcca
     1741 gccgcgtaca gtgtcgtcga tctggatact gtcgatctgg cggttccagt attcccagaa
     1801 ggcctccaga tctttcggcc acatgtccgg ccgcacctgc agtgtcgtgc ccagcggttt
     1861 ggcgtattcg taaaattccg ccagctgttc cggcgtcatg ggtccatgaa atttctcgcg
     1921 cgtatccacg tagccccaga acaggcaggc ggccacccag agttgcagct cggggtccag
     1981 cgcgcggtac tgcacgggtg acgtggccgt ggatgtgacc tgcgcatggg cgtggctgat
     2041 ggctttgcga taggccagtt tttcttccgt actgccctgc aacgccaccc ccaggtacgt
     2101 cagcgtggtg cgggtgcgct tgaacgggtg atgcagcagc gaaccggatg tcacccggct
     2161 ctcgatcacg ccatagccca cggcaggatg cgccagctgc atgatggtgt tggcaacccc
     2221 ggccagcagg ccgacgttgt cgaggcaacc gaatatcgcg tcgtccagtt cctgggcggt
     2281 gcggggttcc caacgcgtct ggatgggcgt ggtatgggag cgggtggaac gtgcaacggg
     2341 ttgagcggca gacttcgtca tggacaacac ctggattgaa ggacctgttt ttgaaggatc
     2401 aggcatcata aactgagcaa ttattcagaa aaactcggat atggggctgg tccggaggga
     2461 atgggtgcga cagcaacaag caattgatct aaaacagaaa aaagtaccaa gacaggtgcg
     2521 cgcgcaggcc acgttacagg cgattctgca agcgtctgct caggttttga tccaggaggg
     2581 gtacgccgga ctgaccacga acgcggtggc ggaacgggcg ggcgtgagca tcggctcggt
     2641 gtatgaatat tttcccggta aagaggccat tgtggcggtg ctgaccaccc gggcggtggc
     2701 tgacactctg cgggaaattg aagactcgct caacggcgct gaccccgccg acctgcgggc
     2761 ggccatgaac cagttcgtgc ggagcctgta ccggattacc tgcgagcggc gtgacctgct
     2821 gaagatactg ttgttccagg taccttttgt acacgagatt cctgccatgc aatcgctgcg
     2881 ggcggaattg atccggctgg tgttgctggg ggcgggcagg acccggcatg agcaccagat
     2941 cgaagccagg ccggaaaccc tgtacctcat cggcagcatg acggcggcgg ccttgctaca
     3001 actggccatc acgccaccgc cggggctgga tgtgaaccgc attctcgacg agctggcgga
     3061 gaaagtggtg gactggctgg tggaaaggaa aagctagcct gcctgcacct tgcgcgccgg
     3121 cggtacacac agggcctgca gttccgccat caggctggcg caatcggggc cgctggcaat
     3181 ctgcttctgc tccgtcagca catcctgcag gatctggtga ttggtaaggg gattggccag
     3241 caccacgcgg aacaccacgg tgcgccggcg atcatattct tccggcatca gcgtggtgcg
     3301 tgacacgaag gatttgccgt gggcgcgttc ggttttctgg atgctgcggg tcaggcggtt
     3361 catcacttcc tggatggcat ccacttctgc cggggtggcc actgccagtt tggcgcgcac
     3421 gaaatccggc agatagcggt aggtgagaat attcagttcc ggctcggtga tcagctcgaa
     3481 ttcgttgtcg gccttgatca tgtcggcaaa cagtttcgcc ttttcgatgc cctgatccat
     3541 caggatttca taaccgtcgt ggccgatgat tttcaggccc gactgcacca gcattgccat
     3601 gccggggcgc gagccttcca gcgtgtagct gcccagatcc tttgaaccct tgcggatgat
     3661 gtagtcggcg tggtgttcga tggccgatac gagcgtggga tcgcggaaca gcaccatgcc
     3721 ggcgcccatg ggcacgtaca tctgcttgtg cgcgtcgatg gtgacggaat cggcgcgctc
     3781 gacacctttc aggatgtggc ggtatttttt cgaaaacagg gtgggcccgc cccaggccgc
     3841 atccacatgg aaatgcacac cgatttcctg ggcgatgtcg gccatttcgt cgagccggtc
     3901 cacactgccg gtttccgacg tgcctgccac gccaacgatg gcaaagatcg ccacgttctc
     3961 gccctgcagg cgtttgatct gttctttcag ttcgggaatg cgcgccttgt tcttgttgtc
     4021 ggtcttgatc gtgatcatga aatcacggcc tatgcccagc acgtcggcgg ctttgcccag
     4081 ggaatagtgg ccgcgttccg acaccagcac cgctgcgccg ttgtaaccat aatgtttgag
     4141 tgcgccgtgc aaaccttcgc gctggatgcc gcggaatttt ccgttggggc cgaatttgtg
     4201 gttgcgggca atccacagtg ccgtggtgtt ggcaatggtg ccgccggaac agaacgaccc
     4261 cagggaatga cctggcaggt gcatccactt ggcgtagaac gcgtcgtcct gttcgtagat
     4321 caggcggtgc accatgccca gcacctggcg ttccatcggc gtgaaggcct tggaggtctc
     4381 gattttcacc aggttctggt tcagcgccat catgatgcgc gacagcggca gcatgaaata
     4441 gggcaaggcg gaggtcatgt gaccgatgaa gctgggggag gcggtgtgca ccgactgcgc
     4501 caccagtttt tccatcagga aatcagcctg atcggacacg aagatgggtt tttgcggaat
     4561 ttgcgaatcc tgaaaatcct tttcgagttc tttcagatcc ttttcaaccg ccacgatatg
     4621 ttccgacagg aagccggcca ggttctggga cagcttctga tcgatcttgc ccagtgtgga
     4681 atccggcgcc tcgggtaccg tgaagatacg gtacatggcc tccatactgg cttcggcata
     4741 tcgcggtttg tctgtcatag tgatgacctt caactcaggc tacttcgtcc tgggcggaat
     4801 ctgcggacat cgtgaccggc agttccgggg agggcgacaa tttaaccata aagtcacatt
     4861 tcccgatacc tcttccaccg tacccccgct tttgtctgct aaccatatga ttttgagaat
     4921 aaaatggctt gcagaccgcg gagcttgatg gagcatgacc tgaccacgga agggggtatt
     4981 gtgctgtcac gcgagcatct acgcgaacat ttggcctgca ctttgtctac cacccttctg
     5041 cccctgaact gtagcaactt ttgacccaaa ccgcgctcac tatcgtgcgc cgggcgctaa
     5101 actgttgtga tagcaattat ttttgaggga acgaccatga accgattgtg ccgtggtgca
     5161 ctgggtgctg cactgattct gctgccattg ataacgcagg ccagggtgga gccccgtgaa
     5221 ggtgaagagc ctatcgaagc gaccgaaccc aagatcacca tcgtggaaga ccggcaggaa
     5281 accctgcaga tctaccagtt caatggtcat gtctatggca tccgggttat tccgaaaaac
     5341 ggcaagccct ataacctggt ggataccaat ggcgacggca aattcatcca gaacgccgcc
     5401 gaccgcatcc tggtgcccga gtgggtgctg gtgaaattct gaaaccctgc tgacggagcc
     5461 ccatggccgt ttacacgcag ttaagtgaaa acgaagtcag tgccttcctg cagggtttcg
     5521 ggctggtgcg gctgacgcgt ttacgcggca cggaggccgg cattgaaaac accaattatt
     5581 ttgtcgacgc ggaagatgcg ggtggacgtg cgcacacgct ggtgctgacg ctgttcgagc
     5641 gcggtgcgga tgcgttgcca tattttgttg cgctgaccac gtatctggcg gagcagggat
     5701 tgccggtgcc at
//
LOCUS       DASUEG010000639         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_290024, whole genome shotgun sequence.
ACCESSION   DASUEG010000639 DASUEG010000000
VERSION     DASUEG010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290024"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFX11_12040"
     CDS             complement(<1..710)
                     /locus_tag="VFX11_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HEX4939398.1"
                     /translation="MKMPWTKLRELGIVGMNQRNASYIMPYNKRKYYPLVDNKLLTKD
                     RAIKRGIPVPKLYCSIELEHQIAHLKDVLAKHDDFVIKPANGSGGNGIIVIAGKTPGG
                     YFRKANDALVSITELEHHVSNILSGMYSLGGDTDSAIVEYRVKFDPCFEHVSYKGVPD
                     LRIIVFRGIPVFAMVRLPTRHSDGKANLHQGAVGAGIDLATGRTIRGVMSSRQVEMHP
                     DTGTSVAGLAIPRWPEIL"
     gene            complement(707..2248)
                     /locus_tag="VFX11_12045"
     CDS             complement(707..2248)
                     /locus_tag="VFX11_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HEX4939399.1"
                     /translation="MSKYKIQFIALILILIAGGAVYYKSTRMGMPLFPSQQSDVWTVE
                     AKLQFHATGGPVKAEFYIPRNPPGFVKLNEDFISSNYGLATEVVDENRKALWAVRRTK
                     GKQVLYYRMELTPDDSGDFEPITSARPRYPTVPTHDETMDAAINAVLKDTRDHSADVV
                     SFTRELLARLNNPNPDQNISLINKVGHGSVEWVRQLINILATARIPARIVYVLPLQDR
                     VKHGQLVPWIQVHNDFEWIAFNPVNGAQGLTSDVLVWRTGDDPLVTIKGGKPAEVDFS
                     ISRHAHSQVSVAEQWARQSSSRLMDFSLFALPVQTQNVYRIILMVPLGALVIVFLRNL
                     VGLATFGTFMPVLVAMAFRETSLIAGVIMFTTLVAIGLILRKFLEDLHLLLVPRLAAV
                     LTIVIMMLAAFSIISVQLGIERGLSVALFPVVILAMTIERMSIVLEETGSKEAFKLGF
                     GSLVAAIIGYAVMSHELLSHLIFVFPELLLVVLALTMLLGRYTGYRLLELWRFRSLMK
                     DADKL"
     gene            complement(2245..2868)
                     /locus_tag="VFX11_12050"
     CDS             complement(2245..2868)
                     /locus_tag="VFX11_12050"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX4939400.1"
                     /translation="MTLVASNAGNLLAEPEVKQRTIYGWVEWIQLQPSQKYTKAKFDT
                     GAKTSSMHALNIEWFEKGGEDWVRFQFSPNTKISAKRILAGKTKNFITIEAPVVRNAL
                     IKQHKRSSAERPVIHQKFILDGKEYEGEFTLTDRRRFIYPILFGRRFLQDVAIIDPAH
                     TFLRGRPAAKEKAAPPSAQKTATHTEDETTLAKTKTESTVPAASKHP"
     gene            complement(3025..3648)
                     /locus_tag="VFX11_12055"
     CDS             complement(3025..3648)
                     /locus_tag="VFX11_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX4939401.1"
                     /translation="MTQSDLFSDLQLQSVELIEIDDGDILWIKRFLPKADADTLLQTL
                     QAEIPWRQDSIRIAGKDTPIPRLQAWYGDPGMTYSYSGLKLDPLPWTPTLAAIKQRIE
                     DYSDCHFNSLLANLYRNEQDSVGWHADDEPELGQNPAIASLSLGDTRDFQLKHRTRPE
                     QKLSLPLQHGDLLIMKGALQHHWVHQIPKSKTPCNPRINLTFRRIFK"
BASE COUNT          699 a         1021 c         1333 g          862 t
ORIGIN      
        1 tgcaggattt cgggccagcg ggggattgcc agacccgcca ccgacgtgcc ggtatccggg
       61 tgcatttcca cctggcgcga ggacatgacc ccgcgaatcg tccggccggt ggccaggtcg
      121 atgccggcac ctacggcgcc ctggtgcagg ttggccttgc cgtcggaatg gcgggtgggc
      181 aggcggacca tggcgaacac ggggatgccc cggaacacga tgatgcgcag gtcgggtacg
      241 cccttgtagc tgacatgctc gaagcaggga tcgaacttga cccggtattc gacaatggcg
      301 ctgtcggtat cgccgccgag gctgtacatg ccgctgagaa tgttggaaac gtggtgttcc
      361 agttcggtga tgctcaccag ggcgtcgttg gccttgcgga agtagccacc gggagtcttg
      421 ccggcgatga caatgatgcc gttgccgccg ctgccgttgg cgggcttgat gacgaagtcg
      481 tcgtgtttgg ccagcacgtc cttcaggtgg gcgatctggt gttccagttc aatgctgcaa
      541 tacagcttgg gcaccgggat gccgcgtttg atggcgcgat ccttggtcag cagcttgttg
      601 tccaccagcg ggtaatactt gcgcttgttg tagggcatga tgtagctggc attgcgctgg
      661 ttcatgccga cgatgcccag ctcgcgcagt ttggtccagg gcattttcat agtttgtcag
      721 catccttcat caggctgcgg aagcgccaca gttccagcag gcggtaaccg gtgtagcggc
      781 ccagcagcat ggtcagtgcc agcactacca gcaacagttc ggggaagaca aagatcaggt
      841 ggctgagcag ttcgtgcgac atcacggcat aaccgatgat ggccgccacc aggctgccga
      901 agcccagttt gaaggcttcc ttcgagccgg tttcctccag cacgatggac atgcgctcga
      961 tggtcatggc caggatgacc acggggaaca gggccacgga caggccgcgt tcgataccca
     1021 gctgcacact gatgatgctg aacgccgcca gcatcatgat cacgatggtc agtaccgcag
     1081 ccagtcgtgg taccagcagc aggtgcaggt cttcgaggaa tttgcgcagg atcagtccga
     1141 tcgccaccag ggtggtgaac atgatgacgc cggcaatgag cgaggtttca cggaaggcca
     1201 tggccaccag gaccggcatg aaggtaccga aggtcgccag ccccaccagg ttgcgcagga
     1261 acacgatcac gagggcgccc agcggcacca tcaggatgat gcggtagacg ttctgcgtct
     1321 gcacgggcag cgcgaacagc gaaaaatcca tcaggcgtga gctgctctgg cgtgcccact
     1381 gttcggccac cgacacctgg gagtgggcgt ggcgtgatat ggagaaatcc acttccgccg
     1441 gtttgccgcc cttgatggtg accagaggat cgtcaccggt gcgccagacc agtacgtcgg
     1501 acgtcaggcc ctgcgcgcca ttgacggggt tgaaggcgat ccattcgaag tcgttatgca
     1561 cctggatcca gggtaccagc tggccgtgct tcacccggtc ctgcagtggc agcacgtaga
     1621 caatgcgggc gggaatgcgg gcggtggcca ggatgttgat gagctggcgc acccattcga
     1681 ccgagccgtg gcccaccttg ttgatcaggg agatgttctg gtccgggttg gggttgttga
     1741 ggcgggccag cagttcccgg gtgaagctca ccacgtcggc agaatggtcg cgggtgtcct
     1801 tcagcacggc gttgatggcg gcatccatgg tttcgtcatg ggtgggaacc gtgggatagc
     1861 gcggccgggc actggtgatc ggttcaaaat cgccggaatc gtcgggggtc agttccatgc
     1921 ggtagtacag cacctgtttg cccttggtgc ggcgtacggc ccagagcgcc ttgcggtttt
     1981 cgtccaccac ttcggtggcc agaccgtagt tgctggagat gaaatcttca ttcagcttga
     2041 cgaagccggg cgggttgcgc gggatgtaaa actcggcctt gaccggcccg ccagtggcgt
     2101 gaaactgcag tttggcttct acggtccaga catcggattg ctgcgatgga aacaggggca
     2161 tccccatgcg ggtggatttg taatagaccg ctccgccggc aatgaggatc aggatcagcg
     2221 caataaactg gattttgtat ttgctcacgg atgtttgctc gcagcaggaa cagtggactc
     2281 ggtcttggtt ttggctagcg tggtttcgtc ttcagtatgt gttgctgttt tttgggcgct
     2341 gggcggggcg gctttttcct tcgctgccgg tctgccgcgg aggaaggtgt gggctggatc
     2401 aatgatagcc acgtcctgca gaaagcggcg gccgaacagg atcgggtaaa tgaagcgtct
     2461 gcggtcggtc agggtgaatt cgccctcgta ttccttgccg tccaggatga atttctggtg
     2521 aatgacggga cgttctgcgc tggaccgctt gtgctgcttg atgagggcgt tgcgcaccac
     2581 gggtgcttca atggtgatga agttcttggt cttgcctgcc agtatccgct tggcggaaat
     2641 cttggtgttg ggggaaaact ggaaacggac ccagtcctcg ccgccttttt caaaccattc
     2701 gatgttgagt gcgtgcatgg acgaggtctt ggcgccggtg tcgaacttgg ccttggtgta
     2761 tttctgcgac ggctgcaatt gaatccattc cacccagccg tagatggtgc gttgcttgac
     2821 ttccggttca gccagcaggt tgccggcatt ggacgcaaca agtgtcaaaa gagcccacct
     2881 tagcagtttt tgggttcgca acagcatatg gatcggatcg gcctgtggga ttcaattggt
     2941 tttcagtata gcgaccgcgg tcgggcggtg aggcgccgat tttaatggca ggtgaatgta
     3001 aacgcacctg aattcctaag tccactattt aaaaattcgc ctgaatgtca ggttgatccg
     3061 gggattgcag ggtgtcttgc ttttgggaat ctggtgcacc cagtgatgtt gcagggcgcc
     3121 tttcatgatc agcaggtctc cgtgttggag ggggaggctg agcttttgtt ccggccgggt
     3181 gcggtgttta agctggaagt cgcgggtatc gcccagggag agggaggcga tggcagggtt
     3241 ctggcccagt tcgggttcgt cgtcggcgtg ccagccgaca ctgtcctgtt cgttccggta
     3301 gaggttggcc agcaggctgt tgaagtggca gtcgctgtag tcctcgatgc gttgtttgat
     3361 ggcggcgagg gtgggtgtcc agggcagcgg gtcgagtttg aggccggagt agctgtaggt
     3421 catgccgggg tcgccgtacc aggcttgcag ccgggggatg ggggtgtcct tgccggcgat
     3481 gcggatgctg tcctggcgcc agggtatttc ggcttgcagg gtctggagca gggtgtcggc
     3541 gtcggctttg ggcaggaagc gtttgatcca gaggatgtcg ccgtcgtcga tttcgatcag
     3601 ttcgacgctt tggagttgga ggtcgctgaa aaggtcggat tgggtcatgg ttcgtggtga
     3661 gcttgcggac ggagggtgcg gaggttcggg gcacgccaca agtacttccc tgtaggctcg
     3721 acagcggcca tccatggccg ctgacgtccc cgaacctccg caccctccgc ccgcaagctt
     3781 cggctcccgt gcgagttcag tgttaaaacg gtaccgcaga actcactttt acgccatgtc
     3841 ctgacaccgg atggcggaac tccagggcgg cggcatggag cagcaggcgg ggactcatgg
     3901 actgcacctc cgggg
//
LOCUS       DASUEG010000640         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_910095, whole genome shotgun sequence.
ACCESSION   DASUEG010000640 DASUEG010000000
VERSION     DASUEG010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910095"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..1150
                     /locus_tag="VFX11_12060"
     CDS             455..1150
                     /locus_tag="VFX11_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939402.1"
                     /translation="MSLLQSAPDVPLAPAPWHLTGSGYILALRMPGNLLDDPTFIPNS
                     LQGSRKGKLAYVMFVDYSASDAGPYHELLYIPGSFRFNQSRHLSITRIYVSTWDSVVN
                     GRNNWGIPKNRCNFAVDYGTEQDNIRLTTDAGTLIAELTLRTRRCFRIPTTTAMVPEK
                     LRTLGQHLDGQQFLYAPSSSGHIKPAQLLDVRCNPELFPDISQGQVVACLRVTDFKMV
                     FPTARVESIAGGQ"
     gene            1219..1755
                     /locus_tag="VFX11_12065"
     CDS             1219..1755
                     /locus_tag="VFX11_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX4939403.1"
                     /translation="MALKSTIFKADVQISNMDANYYATHSLTLAQHPSETEERLMLRL
                     LAFMLHASDRLEFGKGLSNEEEAALWEKDLTDHVTLWIEVGTPDEKRVRKACHRADRV
                     IIYAYGSRAVPVWWQQNQKDLERFDNLSVFGVSKAFSDQLTAMCERTMQLQCTIQDGM
                     AWISNAKDTLQVELEQLK"
     gene            complement(1776..1889)
                     /gene="cydX"
                     /locus_tag="VFX11_12070"
     CDS             complement(1776..1889)
                     /gene="cydX"
                     /locus_tag="VFX11_12070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEX4939404.1"
                     /translation="MWYFTWILGVLLACAFGIVNVLWLEVTENLDRPTLED"
     gene            complement(1917..>2257)
                     /gene="cydB"
                     /locus_tag="VFX11_12075"
     CDS             complement(1917..>2257)
                     /gene="cydB"
                     /locus_tag="VFX11_12075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX4939405.1"
                     /translation="FPLLGLGLPFITAILASRQREGTAFFTSSLAVASIIATAGIALF
                     PFVLPSSLNPHHSLTMWDATSSALTLNIMLLVACVFVPAVLGYTLWSYAKMWGRISTD
                     HVAQHSHSLY"
BASE COUNT          516 a          718 c          605 g          418 t
ORIGIN      
        1 atccggtgct acaaattggc cactgacctg cacgctaggt ttgtccggca ccagggtgta
       61 atcatccccg ccactgccct ttcccccacc gccaccacac gcggccagca acaaagacaa
      121 aagcacaccg ggcgttacgc gccgggtaaa gctgaaataa aagcctgaca tattaactcc
      181 ttgaataaat tattcctgac cggatgcgcc caagcctaaa gacctgctcg cagcgcattc
      241 gcgacgccca gtttagccaa tatccgtcag caatgattcc ggagacatga atatctcagt
      301 gggaaaaata cacaaaaaat ccgaccattc cctaacgcca tgacaatacg gacactgttg
      361 aagtccccat ctaccgcact gactttcgtc ccttccaggg ttatcattcc cacgcgaccg
      421 cttgcccatc cgccctgttt ttccggtgaa ccccatgtcc ctgctgcaat ccgcacccga
      481 cgtccccctt gccccggccc cctggcacct cacgggcagc ggctatattc tggcgctgcg
      541 gatgccgggc aacctgctgg acgatccgac tttcattccc aacagcctgc aaggttcccg
      601 caaaggcaaa ctggcctatg tgatgtttgt ggactacagc gcatcggatg ccggccccta
      661 tcacgaactg ctgtacattc ccggcagttt ccgtttcaac cagagccggc acctgtcgat
      721 tacgcggatt tatgtgtcca cctgggattc ggtggtgaat ggccgcaaca actggggcat
      781 ccccaagaac cgctgcaact tcgccgtcga ctacgggacg gagcaggaca acatccggct
      841 gacgaccgac gcaggcaccc tgatcgcgga actgaccctg cgcacgcggc gctgttttcg
      901 catccccacc accacggcaa tggtgccgga aaagctgcgc acgctgggcc agcacctgga
      961 cggacagcaa ttcctgtatg cgccgtccag cagcggccat atcaaaccgg cgcagttgct
     1021 ggacgtccgt tgcaatccgg aattatttcc cgatatcagc cagggccagg ttgttgcctg
     1081 ccttcgcgtg accgatttca aaatggtgtt tccaacagcc cgggtggaat ccatcgcggg
     1141 cggacaataa accctttatg acggtggccg ttcagcgcat actgcgcagg attttccacc
     1201 accagctgca cagtcgccat ggccctgaaa tccaccattt tcaaagcgga tgtccagatt
     1261 tccaacatgg acgccaacta ttacgccacc cattccctca cgctggcgca acatccctcc
     1321 gaaaccgaag agcggctgat gctgcggctg ctggctttca tgctgcacgc cagcgaccgg
     1381 ctggaatttg gcaagggcct gagcaatgag gaagaagcgg cgctatggga aaaggatctg
     1441 accgatcacg tgaccctgtg gatcgaagtg ggcacgccgg atgagaagcg cgtacgcaag
     1501 gcctgccacc gtgccgaccg cgtgatcatc tacgcctacg gcagccgcgc cgtaccggtc
     1561 tggtggcagc agaaccagaa ggatctggag cgcttcgaca acctgtcggt gttcggcgtc
     1621 agcaaagcct tcagcgacca gttgacggcc atgtgcgaac gcaccatgca gctgcaatgc
     1681 acgatccagg acggcatggc ctggatatcc aacgcgaagg atacgctgca ggtggagctg
     1741 gaacagctca agtagccact tcgcgacacc ggccttcaat cctcaagcgt gggccggtcc
     1801 agattttccg tcacttccag ccacagcacg ttcacaatgc cgaacgcgca ggccagcagt
     1861 acgcccagaa tccaggtgaa ataccacata acttgtttct ccttccggtc gcaggatcaa
     1921 tacagtgaat gggagtgctg cgcgacgtgg tccgtgctga tgcggcccca catcttcgcg
     1981 tagctccaga gggtgtaacc cagtaccgcc ggcacgaaca cacaggccac cagcagcatg
     2041 atgttcaggg tcagcgcact ggaggtggcg tcccacatcg tcaggctgtg atggggattc
     2101 aggctggacg gcagcacgaa cggaaacagc gcaatgcctg cggtggcgat gatgctggcc
     2161 accgccagac tgctggtgaa aaacgcagtg ccttcgcgct ggcggctggc caggatcgca
     2221 gtaataaagg gaaggccaag ccccagcaaa ggaaacg
//
LOCUS       DASUEG010000641         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_228836, whole genome shotgun sequence.
ACCESSION   DASUEG010000641 DASUEG010000000
VERSION     DASUEG010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228836"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFX11_12080"
     CDS             <1..627
                     /locus_tag="VFX11_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX4939406.1"
                     /translation="TSHPLEFSDALIDVYAREPKLVSHLHLPVQSGSDRILALMKRNH
                     TIADYIARIRKLQQVRPGISLSSDFIIGFPGETEDDFLATMHLIDDIGFDTSYSFVYS
                     PRPGTPAADMVDDTPEEEKKRRLALLQHRIIQNAQGISRRMVGTVQRILVDGISKKDP
                     GQLQGRTENNRVVNFHHHDDDLVGQFVDVKIEEALPNSLRGILVAVIG"
     gene            complement(661..1530)
                     /locus_tag="VFX11_12085"
     CDS             complement(661..1530)
                     /locus_tag="VFX11_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4939407.1"
                     /translation="MSVPVFVLLSASILWGLTWLPLKFLHGRGIEGLPLILVSYGAVA
                     LLLAPLLWRQRSCWTGGAGALGLIFLLGGCANLTFNLALMHGDVVRVMVLFYLMPMWS
                     AAGGRLFLGEAIDRQRLVSVALALGGALLILGGWNILEQPLGWLDLLALVSGLTFALN
                     NIAFRASPALPLATKVGAMFTGCAALTGLLLVAGVQPDLAASPAWQPAAFGWMALMGL
                     IWFLASLGTLWAVIRLQAGQAGVIMVMELLSAVVSAAWLGESVLTTLKLAGVVCVLAA
                     ALLEAWRPGAAGH"
BASE COUNT          472 a          727 c          545 g          275 t
ORIGIN      
        1 accagtcacc cgctggagtt ctccgatgcg ctgatcgacg tctacgcccg cgaacccaag
       61 ctggtgagcc atctgcacct gccggtgcaa agcgggtccg accgcatcct ggcgctgatg
      121 aaacgcaacc acaccatcgc cgactacatc gcccgcatcc gcaaactgca gcaggtgcgc
      181 cccggcattt ccctgtcgtc ggatttcatc atcggtttcc cgggcgagac cgaagacgat
      241 tttctcgcca ccatgcacct gatcgacgac atcggcttcg acaccagtta cagcttcgtc
      301 tacagcccgc gccccggcac cccggcagcg gacatggtgg acgacacgcc ggaagaggaa
      361 aagaaacgcc ggctggcact gctgcagcac cgcatcatcc agaacgccca gggcatcagc
      421 cgccgtatgg tgggcacggt gcagcgcatt ctggtggacg gcatttcgaa gaaagacccg
      481 ggccagctgc agggccgcac cgagaacaac cgggtggtga acttccacca tcacgacgac
      541 gatctggtgg gtcagttcgt ggatgtgaaa atcgaggaag cgctgcccaa ttccctgcgt
      601 ggcattcttg tggccgtaat tggataacgc gaagccggat accgcacgcc tgtacccggt
      661 tcagtgtccc gccgccccgg gccgccaggc ttccagcagc gcagccgcca gcacacacac
      721 cacacctgcc agtttcagcg tcgtcaggac cgattccccc agccaggctg ccgacaccac
      781 ggcagacagc aactccatca ccatgatcac gcccgcctgc ccggcctgca gccggattac
      841 cgcccacaag gtacccaggc tggccaggaa ccagatcagc cccatcagcg ccatccagcc
      901 gaacgctgcc ggttgccagg ccgggctggc ggccagatcc ggctggacac ccgccaccag
      961 cagcaacccg gtcagagccg cgcaacccgt gaacatggca cccaccttgg ttgccagcgg
     1021 cagcgctggc gaagcgcgaa aggcaatatt gttcagtgcg aaggtcagcc ccgacaccag
     1081 cgccagcagg tccagccagc ccagcggctg ctccagaata ttccacccgc ccagaatcag
     1141 cagcgcgccg cccagcgcca gggccacact caccagccgc tgccggtcaa tggcttcgcc
     1201 cagaaagagc cgtccccccg ctgcactcca catcggcatc aggtaaaaca gcaccatcac
     1261 ccgcaccaca tcgccgtgca tcaatgccag attgaaggtc aggttggcac agccccccag
     1321 caggaagatc aggccgagcg caccggcccc gcccgtccag cacgaccgtt gccgccacag
     1381 cagcggcgcc agcagcagcg ccaccgcccc gtagctcacc aggatcaggg gcaggccctc
     1441 gatgccccgc ccatgcagga acttcagcgg cagccaggtc aggccccaca ggatggatgc
     1501 agacaacaac acgaaaaccg gcacagacac ctcacggcaa accagcaaca ggaatggcgg
     1561 gcatgataca gaaaacaggg atgcaggata aaaaaggatg gacagggagg agatcacaag
     1621 gtgggtcgag gcggcgggtg gtacaacaca actccacccg ccgcttcttc ccgatttccg
     1681 acgtgccttt cgccgtcaga cgacgctaca gcgcagccag gtacaactta cttcacccgg
     1741 cagatgcaaa acccgcagcc gggcgcaaca acgagtgcgc caccggccag aaaagcccca
     1801 attcgggttt cgtcgacttt cccccttgaa ttcggtatcg cgggaaaggc atcgaaatca
     1861 aaccttgcgg ggaccatagc agcacccgtg ccaactatcc aacctgttga tttaaaagac
     1921 tataaaacca atctcatcac agccgcgcag ggggcctgta aaccctgccg acagaaaact
     1981 ccccccgccg cgccaaaccg ccggcacccc cgtaaacca
//
LOCUS       DASUEG010000642         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1280683, whole genome shotgun sequence.
ACCESSION   DASUEG010000642 DASUEG010000000
VERSION     DASUEG010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280683"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..818)
                     /locus_tag="VFX11_12090"
     CDS             complement(285..818)
                     /locus_tag="VFX11_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4939408.1"
                     /translation="MARIKREQDRDEKRDEIVAAARLLFVEEGYEATSISRLASAAGV
                     APNTIYWYFKDKDEVLVAVLDAELLARMGEYLVLPTMSPAERLLWVANQLERVSRLVG
                     TVHSRLAQSPAINTWHDRFHAFSEEMLRLELGQAGIAPQKIAALVKISVFTIEGLLAH
                     PLPEEQKRAICAALVTL"
     gene            957..1526
                     /locus_tag="VFX11_12095"
     CDS             957..1526
                     /locus_tag="VFX11_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939409.1"
                     /translation="MSAVISAAVLSQLSTQELLQLFATLEAPGISEMQGEYSARLLAQ
                     PFWLAKLVGRAVLENPFQTWLCKAFRPVDDATGRGYNTFQQGRRVVQRYPMLTLMGPS
                     RFDGRPAYQLIYRHFHSACGAVHMVDEVRRAAPGLYLGMGTYGFTDAQRRVPYPFLLQ
                     GPVAAYRGDIGRRRDNFVIGPRELPARFA"
     gene            1647..2573
                     /locus_tag="VFX11_12100"
     CDS             1647..2573
                     /locus_tag="VFX11_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4939410.1"
                     /translation="MINRRNPDTSDITTRVIKSTFVAEGDTCSAMLHVPDGAGDTQLP
                     GILMVGGWGSVQQALTISFVKRFVAAGYAVMEFDFPGWGLSGGWPRQDINPWRRVKAA
                     NAALAHLKNQPQVAAHRIVVWGTSFGGGHVVDLATQHPDLQGAIIQVPMLDGLVTLRA
                     TPLPRMVQLLSLGVAGLLKPGPAIQVPTVARKGEFGTMDRDNAWDALKMGLDAHPGAK
                     YVNRVTARSVLTIGFYRPWTRLKDVQIPMLIVGATRDTVAPFVEDKVRKVANPHLSVV
                     QIDADHFDPYFEPYFPQTLEPQLGFLNKVMPI"
     gene            2590..4068
                     /locus_tag="VFX11_12105"
     CDS             2590..4068
                     /locus_tag="VFX11_12105"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004747846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4939411.1"
                     /translation="MNAQVDVKDTPGIAIIGTGFGGLGLAIQLKKAGFNNVTLFEKAG
                     DVGGVWRDNTYPGAACDVPSHLYSFSFEQDLGWQNRFGKAEEIHQYLRHCADKYGIRP
                     HIRFNTEIAGAEFDEEAALWTLRTTSGETHRAQVMVAAVGQLNRPAYPKLKGLDSFTG
                     KAFHSARWDHDYDLTGKRVAVIGTGASAIQFVPEIVAQVTHLDIYQRSAPYVLPKPDK
                     QYPGLEKALFRNVPLLQTLDRAWQYSYHELRQLGFTSQINEMAIVEHVFQKHIREIVK
                     DNKLRHRLTPDYPIGCKRILISNHWYKALIEPNVDVISEDIAEVVPNGIRTTDGRERE
                     VDTIIYGTGFTATDFLAPMQIIGLKGQTLKQAWKDGAEAFLGLTVSGFPNLFMLYGPN
                     TNLGHNSIVYMLESQISYIVDALRKFQRNGLRYLDLRRDVQDVFNQEVQERMKSTVWA
                     QGCTSWYQTAAGKNTNNWPGFTFEYRNRTRKLDLDHYVQVRA"
     gene            complement(4212..>4948)
                     /locus_tag="VFX11_12110"
     CDS             complement(4212..>4948)
                     /locus_tag="VFX11_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939412.1"
                     /translation="VAGGNMKRKRISLINDECEMATANPAQDCPEQFTGWGPLLPATR
                     YQWYTPAGSGSAEKWQQSDKAPDAEFENTEIVQTLIDVQGRVLRLVRPTASDGDNPLP
                     EWWLVRSSSDGSHWQRLPLPQGMAAINDNVDQSVCAQGCRLKLATDPRHPQAELLFLY
                     EGTLDDGYIAQRFDRDKYHTDTDRPSAPLHVWWLDESASPAQWRDVLRADEDSVRRQP
                     LALTGALSGIQSYGWHLAQPVLWVSQ"
BASE COUNT         1028 a         1486 c         1396 g         1038 t
ORIGIN      
        1 cctgattaat acgacaattg acgtagttgt agtagcgaac tccaatgaga ctgtagccgg
       61 aaatgtaatg ttgttacaag cgagtgatgt ttttaaaagc ccgatccact cagtttagag
      121 attctcctta gggcgagaag gagactagtg acagatataa tttatccttg ccagcctgaa
      181 aaagcggacg cgcctcaggc tacacattcc aacttcaccc gaacactctg accgtcctga
      241 acagcagaat gcactcccct gctgccagta ggcttctcgc cagatcagag agttaccaac
      301 gctgcgcaga tcgcacgctt ctgctcctcg ggaagaggat gagcgagcaa gccctcgatg
      361 gtgaatacgc tgattttcac caaggccgcg attttctgcg gcgctatgcc ggcttgccct
      421 aactccaggc gcagcatttc ctcactaaat gcatgaaagc gatcatgcca ggtgttgatg
      481 gcaggcgact gcgccaggcg ggaatgtacg gtacccacca ggcgactgac ccgttcaagc
      541 tggttggcaa cccagagcag gcgctcagca gggctcatgg tcggcagcac cagatattcg
      601 cccatgcgcg ccaagagctc ggcatccagc acggcgacca gcacctcgtc cttgtccttg
      661 aagtaccagt agatggtgtt gggcgcgacc ccggcggcgc tggccaggcg gcttatcgag
      721 gtggcctcat agccttcctc cacaaacaat agtcgggccg cagcaacgat ctcgtcccgc
      781 ttttcatctc ggtcttgctc gcgcttgatt cgcgccatcc ttttcactcc tgttggccag
      841 tcgggaggag tatcccgacg aaagcgcatg atgggtatta tatctcctgt tgaatatcgt
      901 tcaacatgtg ggttagtatt gtgttgaacg atattcaaca caatacaggg ggcgccatga
      961 gtgcggtcat atcggcagca gtcttgagtc agctttcgac gcaggaactg ctgcaactgt
     1021 ttgccacgtt ggaggcgccc ggtatttcgg aaatgcaggg ggaatattca gcgaggctgc
     1081 tggcgcagcc cttctggctg gccaagctgg tcggccgggc agttttggag aatcctttcc
     1141 aaacctggct gtgcaaagcc ttccgcccgg tcgatgacgc aaccgggcgg ggctacaaca
     1201 cgtttcagca gggccgtcgc gtcgttcagc ggtatccgat gctgacgctg atgggacctt
     1261 cccgcttcga tggccgtccg gcgtaccagc tcatctatcg gcatttccac tccgcctgcg
     1321 gtgcggtgca catggtcgat gaggtccggc gtgccgcacc ggggctctat ctcggcatgg
     1381 gcacctatgg ttttaccgac gcccagcgcc gcgttcctta tcccttcctt ttgcagggtc
     1441 cggttgccgc ctatcgcggc gacatcggcc gccggcgcga caactttgtc atcggaccgc
     1501 gggaactccc cgcgcgattt gcctgatgag gattcccatg aaaacaagca accgcctgaa
     1561 tccgttcaag gagcattgag ggcgttttat tcagagtctc aggagcaacc gggactcata
     1621 actaaacagc aatcacagag gtaaatatga tcaaccggcg caatcccgat acgtctgaca
     1681 ttacaaccag agtcatcaag tccacctttg tcgcggaggg cgacacctgc tccgccatgc
     1741 ttcacgttcc agacggagca ggcgacacgc aactgccggg aatcctgatg gtcggcggct
     1801 ggggcagcgt gcagcaggcg ctgacgattt ctttcgtgaa gcgttttgtg gccgcaggct
     1861 atgccgtcat ggaattcgat tttcccggct ggggtttgag tggcggctgg ccgcgtcagg
     1921 atattaatcc ctggcggcgc gtaaaggcgg caaacgcggc actggcacat ctcaaaaacc
     1981 agcctcaggt ggccgcgcac cgcattgtgg tgtggggcac gtcgtttggt ggcggccacg
     2041 tcgtcgatct tgcgactcag catcccgatc tgcagggtgc catcatacag gtgcccatgc
     2101 tggacggcct ggtgacgcta cgtgccacgc ccctgccgcg gatggtccag ctcttgagtc
     2161 tcggtgtcgc tggattgctg aagcccggcc ccgcaattca agtcccgacg gtggcacgta
     2221 aaggcgagtt cggtaccatg gaccgggata atgcctggga tgcgctgaaa atggggctcg
     2281 atgcgcaccc cggtgcaaag tatgtgaacc gtgtgaccgc tcgctcggtg ctgacgattg
     2341 ggttctaccg cccctggacc cgcctcaagg acgtgcagat tccgatgctg atcgtgggtg
     2401 cgacccgtga cacggtggcg ccattcgtcg aagacaaagt gcgcaaggtg gccaaccccc
     2461 atctcagcgt ggttcagatc gatgcggatc atttcgatcc ctactttgag ccgtacttcc
     2521 cccagacgct cgaacctcag ctgggcttcc tgaataaagt gatgccgatc taacctccgg
     2581 agacgccccg tgaacgcaca ggtcgatgta aaagacaccc ctggtattgc catcattggt
     2641 actggctttg gcggccttgg actcgccatc cagcttaaga aggcaggatt caataacgtt
     2701 acgctgttcg agaaggctgg cgacgtcggc ggcgtctggc gcgataacac ctatccgggc
     2761 gccgcctgcg atgtgccgtc ccacctttac tccttctcct ttgagcagga ccttggctgg
     2821 cagaaccgtt ttggcaaggc ggaagagatc caccagtacc tccgtcattg cgccgacaag
     2881 tacggaattc gcccgcatat ccgcttcaat accgagattg ccggcgccga attcgacgaa
     2941 gaagccgccc tgtggacgct gcgcaccacc agcggcgaga cacaccgggc tcaggtcatg
     3001 gtggctgccg tgggacagct caaccgcccg gcctacccga aactgaaagg cttggacagt
     3061 ttcacgggca aggctttcca ttcagcccgc tgggatcatg actatgatct gactggcaag
     3121 cgcgtggccg tcatcggaac cggtgccagc gccatccagt ttgtgccgga gatcgtcgcg
     3181 caggtcaccc atctcgatat ctaccagcgt tcggcgccgt atgtgctgcc gaagcccgac
     3241 aagcagtacc ctggacttga aaaggcgctg tttcgcaacg ttcctttgct gcagacgctg
     3301 gatcgtgcct ggcaatacag ttaccacgag ctgcgccaac ttggctttac ctcccagatc
     3361 aacgaaatgg ccattgtcga gcatgtgttc cagaagcaca ttcgtgaaat cgtcaaggac
     3421 aacaaactcc gccaccgcct gacaccggac taccccattg gctgcaagcg catcctgatt
     3481 tcaaaccact ggtacaaagc gctgatcgag ccaaacgtcg acgttatcag cgaggacatc
     3541 gcggaggtgg tgcccaacgg cattcgcacc acggacggca gggagcggga agtggacacg
     3601 atcatctacg gcaccggctt caccgcgacc gacttcctgg cgccgatgca gatcatcggc
     3661 cttaaaggcc agacactgaa acaggcttgg aaggatggag ccgaagcatt cctgggcctc
     3721 accgtgagtg gcttccccaa cctgttcatg ctttacggcc cgaacaccaa tctgggccac
     3781 aattccatcg tctatatgct ggaaagccag atcagctaca tcgtcgacgc cctgcgtaag
     3841 tttcagcgga atggtctgcg gtatctggac ctgcgcagag acgttcagga tgtcttcaat
     3901 caggaagtgc aggaacgcat gaagagcact gtctgggccc agggctgcac cagctggtac
     3961 cagacggctg ccggcaaaaa caccaacaac tggccaggct tcaccttcga ataccgtaat
     4021 cgcacacgca agctggacct ggatcactat gtacaggtca gggcctgagc ctgtcggctg
     4081 gctgagtccg cagggatcta cgaaataagc gtggtcgagg cgaaccaggt attaattggc
     4141 gctcgcaacg cccgctggag agaattcagc ttgcccagtg tttgttatgg gataacggat
     4201 tttctgcaat tctactggct cacccacaag acgggctgcg ccagatgcca gccgtatgac
     4261 tggatacccg agagtgcacc cgtcaatgcc agaggctgtc gacgtacgct atcttcatcg
     4321 gcccgcaaca catcccgcca ctgcgccggg gacgccgatt catccagcca ccagacatgc
     4381 aaaggcgcgg atggcctgtc tgtatccgtg tggtacttgt cgcgatcaaa ccgctgcgcg
     4441 atatacccat catccagggt gccttcatac aggaacagta actccgcttg tggatgccgt
     4501 ggatcagtgg ccagtttcag ccggcaaccc tgcgcgcaca cgctctggtc aacgttatcg
     4561 ttgatcgctg ccattccctg cggtaacggc aggcgctgcc agtggctacc atcactgctg
     4621 ctgcgaacca gccaccattc cggcagcgga ttgtcgccat cgctcgctgt gggtcgcacc
     4681 agacgcagca cacgcccctg cacatcgatg agcgtttgca cgatctccgt attctcaaat
     4741 tcggcatccg gcgctttgtc gctctgttgc cacttttctg cgctgccgct ccctgccggt
     4801 gtgtaccact ggtagcgcgt ggccggcaga agggggcccc agcctgtgaa ctgctccgga
     4861 caatcctgcg cgggatttgc agtcgccatt tcgcattcat cgttgatgag cgatatgcgc
     4921 ttgcgtttca tgttgccgcc agccacca
//
LOCUS       DASUEG010000643         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_378787, whole genome shotgun sequence.
ACCESSION   DASUEG010000643 DASUEG010000000
VERSION     DASUEG010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378787"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX11_12115"
     CDS             <1..279
                     /locus_tag="VFX11_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939413.1"
                     /translation="WFSDLRFCSIPDSALSFLTFISDAIIDWWHKRCQTETTYQLISK
                     THNCTGCVTGALHAAGLGERPSAACVLAAADVLDWELLEGNRQNPIWG"
     gene            473..1066
                     /locus_tag="VFX11_12120"
     CDS             473..1066
                     /locus_tag="VFX11_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939414.1"
                     /translation="MLSRILVFTGAFAAAGAANADLILGAQLGKQSLDIRLTDSFGNV
                     EEDTTESDTALGLIVGVGQPGGGTRIVAEWHSFSIGDEVDLDLLDVSYSYFFPSLTKS
                     SESQLRPFVGGDLGYGWLDVSAIPGYDSGDDSNILYGVRAGLNLAIGSRAELEVGARY
                     TIVDLDAELDSRFAILDPAHYEVENNKGWWIGFNFGI"
     gene            complement(1177..1413)
                     /locus_tag="VFX11_12125"
                     /pseudo
     CDS             complement(1177..1413)
                     /locus_tag="VFX11_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
     gene            1510..1686
                     /locus_tag="VFX11_12130"
     CDS             1510..1686
                     /locus_tag="VFX11_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939415.1"
                     /translation="MLDCKPQLARFLFRARHAVMAGPMREQLEANNRLRYGALLDRLT
                     AGVKAGTVSLKRAV"
     gene            complement(1664..2506)
                     /locus_tag="VFX11_12135"
     CDS             complement(1664..2506)
                     /locus_tag="VFX11_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4939416.1"
                     /translation="MSFKTLTVSIQDKIAHLVLNRPAEFNTMNEDFWRELPQAIREID
                     EKAAARVIVISSTGKHFTAGMALSVFTNPNNISMQGDPARAAEQLRRLVLQLQDAFNV
                     LETVRMPVLAAIQGGCIGGGVDMVSAADCRYCTADAFFCIKETDLGMTADVGTLQRLP
                     HSIPQGMIRELAYTAGKLSAQRAKEIGLVNEVFADQDALLAGVMDIAKEIAKRSPMAV
                     AGCKEMINYSRDHSVGDSLKYMATWQSGMFRPEDMMKAMMAQQQKATPEFPEIFPIKP
                     PFSG"
BASE COUNT          498 a          718 c          760 g          573 t
ORIGIN      
        1 tggttctccg acctcagatt ctgttccatt cccgatagcg ctttgtcctt tttgacgttt
       61 atttcagatg cgatcattga ttggtggcat aagcggtgcc agaccgagac tacctatcag
      121 ctgatcagca aaacccacaa ctgcaccgga tgcgttactg gggcgctgca tgccgccggc
      181 ctgggggaaa ggccgtccgc tgcttgtgtg ctggcagccg ctgatgtcct tgactgggag
      241 ttgctggagg gcaatagaca gaatccgatc tgggggtaaa tctttgtccc gcaacgctac
      301 agctcgcccg attcccgtga aatgaacatc acacagttga cccggatcaa gatcccgcat
      361 agtgacggca gggtgggtat ggtaccttca aatatcgatg atccgaaccc gcatcctgca
      421 ggacaagaca gtagcgggac attcacgctg gcaacagcaa caggaggcaa atatgttgag
      481 ccggattctg gtgttcactg gagcattcgc agcagcaggt gcggcaaatg ccgatttgat
      541 tctgggcgcg caactgggca agcaatcgct ggatatccgc ctgacggaca gttttggcaa
      601 cgtggaagaa gacacgacgg aatccgacac tgcgctggga ttgatcgtgg gcgtcgggca
      661 gccaggaggc ggcacccgca ttgttgccga gtggcacagc ttcagcatcg gtgacgaggt
      721 tgatctggac ctgctcgatg tcagttacag ctatttcttt cccagcctga ccaagtcatc
      781 tgaatcacaa ctgagaccct ttgtaggcgg ggatctgggg tacggctggc ttgacgtatc
      841 cgcaataccg ggttacgaca gcggcgatga cagcaacatt ctgtatggcg tccgcgcagg
      901 ccttaatctg gccatcggca gtcgggcaga actggaggtg ggcgcgcgtt ataccattgt
      961 cgatctcgat gcggaactgg acagccggtt tgcgattctt gatcccgccc actatgaggt
     1021 cgaaaacaac aaaggctggt ggatcggctt taactttgga atttgaaacg gaaccgttcc
     1081 agcggtgact gatcgcgagt ccggggtgct cgttgccccg gactgtcctt ccgcccagtt
     1141 gacgctggcg ggcaagggta tcggaaatgc tgcgactcac gacattttcg gtgtcaccca
     1201 catggtgatc agcgcctgga acacagtctc gccctgttcg tcacgcaccg ctacttttac
     1261 cttgtattcg cctggcgtgg accagtccgg ttttgacatc ggcgtagctg cagccaccag
     1321 atcggtttcg gccttcttca ggtattccac ggtcatgccc ttgggaatcc agcggtgcgt
     1381 tgctggtacg gtgacctcag tcatggtgcc acccggccgg ggtgccagtt tttgccggat
     1441 attcagtgtg ctcatgggtt ggctcatggt gttcctccgc caggcttctg acttggccct
     1501 ttacgtggca tgctggactg caagccgcaa ctggcgcggt ttctgtttcg cgcacgccat
     1561 gccgtgatgg caggccccat gcgcgagcag ctggaggcaa acaaccggct gcgttatggc
     1621 gcgttgctgg ataggctgac agcgggcgtg aaggcgggaa cggtcagcct gaaaagggcg
     1681 gtttgatcgg gaatatctcg ggaaattccg gcgttgcttt ctgctgttgc gccatcatcg
     1741 ccttcatcat gtcctcgggg cggaacatgc cggactgcca ggtggccatg tatttcaggc
     1801 tgtcgcccac gctgtgatca cggctgtagt tgatcatttc cttgcagccg gccaccgcca
     1861 tcggcgagcg tttcgcaatt tcctttgcaa tgtccatcac acccgccagc aacgcatcct
     1921 gatcggcaaa cacttcgttc accagaccga tttccttcgc ccgctgcgcc gataatttcc
     1981 cggcggtgta ggccagttcg cggatcatgc cctgcggaat cgaatgcggc agacgctgca
     2041 gcgtgcccac atcggcggtc atgcccagat cggtttcctt gatgcagaaa aatgcgtctg
     2101 ccgtgcagta acgacagtcc gccgcactca ccatgtcgac accgccgccg atgcaaccac
     2161 cctggatcgc cgccagcacc ggcatgcgca cggtttccag cacgttgaac gcatcctgca
     2221 attgcagcac cagacggcgc agctgttcgg cggcgcgggc cggatcgccc tgcatggaaa
     2281 tgttgttggg attggtgaac acactcagcg ccatgccggc ggtgaaatgc ttgccagtgg
     2341 aggaaatcac gatcacccgc gccgcggctt tttcatcgat ctcacggatg gcctgcggca
     2401 gttcgcgcca gaaatcctcg ttcatggtgt tgaattcggc cgggcggttc agcaccaggt
     2461 gggcgatctt gtcctgaatt gaaacggtca gggttttgaa tgacatgaaa gtctccggat
     2521 ggcgggcaag ggtgcgactg agtatcggc
//
LOCUS       DASUEG010000644         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_685487, whole genome shotgun sequence.
ACCESSION   DASUEG010000644 DASUEG010000000
VERSION     DASUEG010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685487"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFX11_12140"
     CDS             <1..1034
                     /locus_tag="VFX11_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939417.1"
                     /translation="PFHQVDSSSARRFEGSGLGLAICRNLAELMQGRIEVDSTPGQGS
                     CFRLLIPFERGTSPGLNPAPVLVPAVADETLDHLRVLLVEDNDINQELAETLVKASGA
                     SVETVSNGADAVAAVTARHYDLVLMDIQMPGMDGMEATARIRALPQGRRLPVIAMTAN
                     ALPGDREKYLAAGMNDYIAKPIDPAALQRALRTWGASPLPDNAEPDPFASLEQAGVNT
                     RGALEHLMHNGLLYRRLLTRFVEERAELPRQLADALARDARDDAVNLIHALRSLAGTL
                     GLGELEKVAAELEQQLRRQPPDEALIETFERQMRDAITLVRAWLYEQDSGHEPESPRR
                     AGRAGPTVL"
     gene            1094..1435
                     /locus_tag="VFX11_12145"
     CDS             1094..1435
                     /locus_tag="VFX11_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEX4939418.1"
                     /translation="MSTLPACPQCGSEFTYEDGGLFICPECAHEWSAEAAADAGDDVR
                     VIKDANGNLLQDGDTVTVIKDLKVKGSSLVVKVGTKVKNIRLVDGDHDIDCKIDGIGA
                     MKLKSEFVKKS"
     gene            1584..2468
                     /locus_tag="VFX11_12150"
     CDS             1584..2468
                     /locus_tag="VFX11_12150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4939419.1"
                     /translation="MATSAKTASLAGTADIPVRRMDFEFDDAIPKYWFGGDPLRTLLL
                     TALSCTFPEGERMFMRAVRRYQDGITDPVLQKQVRAFIGQEAHHGKEHEAFNAFMGRK
                     GLPTAYVEKYTKIGIGREEQFFSPERMLAKTCALEHFTAMFAETLLEHPDLIADIDDR
                     LKPLWLWHAIEESEHKSVAFDVYQDQVGSYWVRTSEMALTTVLFTFFSALHTRHLLKG
                     DPDAKQHRQGLTKRLTGLWQHRQILKELGTHYLQYYRPDFHPSQKDSSFLRRKGLKKL
                     QEWVGDRAAGAPDLQAQA"
     gene            2595..>2826
                     /locus_tag="VFX11_12155"
     CDS             2595..>2826
                     /locus_tag="VFX11_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4939420.1"
                     /translation="MLIRPARVDDLAVVATFPQTSDELFYVHPVAQFPLTAEQLLPNF
                     QNRKGNTVIESSGAVVAFANYISVTEQGDATIG"
BASE COUNT          552 a          949 c          849 g          476 t
ORIGIN      
        1 agccgttcca tcaggtggac agctccagcg cccgccgctt cgagggcagc ggcctgggtc
       61 tggccatctg ccgcaatctg gcggaactga tgcagggccg catcgaagtg gacagcacgc
      121 cggggcaggg cagctgcttc cgcctgctga ttcccttcga gcgcggcacc agccccggcc
      181 tgaacccggc cccggtgctg gtcccggcgg tggccgacga aaccctcgac catctgcggg
      241 tgctgctggt ggaagacaac gacatcaacc aggaactggc ggaaaccctg gtgaaagcca
      301 gcggcgccag cgtggagacc gtgtccaacg gagccgacgc cgtggcggcg gtgaccgcgc
      361 gccattacga cctggtgctg atggacatcc agatgcccgg tatggacggc atggaagcca
      421 ccgcccgcat ccgcgccttg ccgcaaggcc ggcgcctgcc ggtgatcgcc atgaccgcca
      481 acgccctgcc gggggaccgg gaaaaatacc tcgcggccgg catgaacgat tacattgcca
      541 agccgattga tccggccgcc ctgcagcggg ccctgcgtac ctggggcgcc agtccgctgc
      601 cggacaacgc cgaacccgac cccttcgcca gcctggagca ggccggcgtc aacacccgcg
      661 gcgccctcga acacctgatg cacaacggcc tgctctaccg tcgcctgctc acccgtttcg
      721 tggaggaacg ggccgaactg ccgcgccagc tggccgatgc cctggcccgc gatgcccgcg
      781 acgacgccgt taacctgatt catgccctcc gctccctggc cggcacgctc ggcctggggg
      841 aactggaaaa agtggccgcc gaactggagc agcaactgcg ccggcagccg ccggacgaag
      901 ccctgatcga aactttcgag cgccagatgc gcgacgccat caccctggtg cgggcctggc
      961 tctacgagca ggactccggg cacgagccgg aatcgccgcg tcgtgctggc cgtgccggcc
     1021 caacggtgct ctaatacgcg cgccggttgt ccggtctttc cgtttacccc gattgtctgc
     1081 gaaggaagtc cccatgagca cactgcccgc ctgcccccaa tgcggttccg aattcaccta
     1141 tgaagatggc ggcctgttca tctgccccga atgcgcccat gaatggtcgg ccgaagccgc
     1201 cgccgacgcg ggcgacgacg tgcgcgtcat caaggacgcc aacggcaacc tgctgcagga
     1261 cggcgacacc gtcaccgtga tcaaggacct gaaagtgaag ggttcgtcgc tggtggtgaa
     1321 agtgggcacc aaggtgaaga acatccgcct ggtggacggc gaccacgaca tcgactgcaa
     1381 gatcgacggc atcggcgcca tgaaactgaa atcggagttt gtgaagaagt cctgaccgag
     1441 gctggatgaa ggggggcaaa gtctggccct atttgaccag tcgttggcac cctctgaccg
     1501 atgattcaca gacaggctcc cctatactga aaaccatcgc gccctcccgg cgccgcgttt
     1561 ttccgaccca gaggagcccc tgcatggcca cttccgccaa gaccgccagc ctcgccggca
     1621 ccgccgacat tcccgttcgc cgcatggatt tcgagttcga cgacgccatt cccaaatact
     1681 ggttcggtgg cgacccgctg cgcaccctgc tcctcaccgc cctgtcctgc accttccccg
     1741 agggtgagcg catgttcatg cgtgcggtgc gacgctacca ggatggcatc accgatcccg
     1801 ttttgcagaa acaggtgcgc gccttcatcg gccaggaagc tcaccacggc aaggaacatg
     1861 aagccttcaa cgccttcatg ggccgcaagg gactgcccac ggcttacgtg gagaaataca
     1921 ccaaaattgg catcggtcgt gaggagcagt ttttctcacc cgagcgcatg ctggccaaga
     1981 cctgcgcgct ggaacatttc accgccatgt tcgccgaaac cctgctggaa catccggacc
     2041 tgattgccga catcgacgac cgcctgaaac ccctgtggct gtggcatgcg atcgaagaaa
     2101 gcgagcacaa gtcggtggca tttgacgtgt accaggatca ggtcggaagt tactgggtgc
     2161 gcaccagcga gatggcgctg accacggtac tgttcacctt tttctcggcg ctgcacaccc
     2221 gccacttgct gaaaggcgat ccggacgcga aacagcaccg ccagggcctg acaaaacgcc
     2281 tgaccggctt gtggcagcac cgccagatcc tgaaagaact gggcacgcat tatctgcagt
     2341 attaccgccc ggattttcat ccgtcgcaga aagacagttc gttcctgcgc cgcaaaggcc
     2401 tgaagaaatt gcaggagtgg gtgggcgatc gcgccgccgg cgcgccggat ctgcaggcgc
     2461 aggcgtaatt tcccccggcg ccctgtcacc cgatggggcg cttttcctgc gcgcaatctg
     2521 aagcgcgatc aggacaagcc ggcattcctg cagtaaagtg gcacgctgct gctttccact
     2581 gacaggacgt tgccatgctg atccgccccg cccgcgtcga cgatctcgcc gtcgtcgcca
     2641 cttttccgca aacctccgac gaattgtttt acgtgcatcc tgtggcgcag tttccgctta
     2701 ctgcagaaca gctgctgccg aatttccaga acagaaaagg caataccgtg atcgaatcca
     2761 gcggtgcggt ggtggcgttc gcgaattaca tcagcgtgac ggagcagggc gatgccacca
     2821 tcggca
//
LOCUS       DASUEG010000645         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_555618, whole genome shotgun sequence.
ACCESSION   DASUEG010000645 DASUEG010000000
VERSION     DASUEG010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555618"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..863)
                     /locus_tag="VFX11_12160"
     CDS             complement(123..863)
                     /locus_tag="VFX11_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939421.1"
                     /translation="MSLKNKVAVVTGGGSGIGRAISLQLAQDGAAVSVWDLSGDSAEQ
                     TVAMIAKAGGRAIACVGDASAKSDIAHGAQLTRQAFGPITILVNNAGISSFTPFMDIS
                     EEMIDRIMAINIKGPMLCCQEIIPDMLSAGWGRIINISSSSAQTGAPMMCHYSASKGG
                     VIAFTKSLALELAAKGITVNNIPPGFVDTPMLRKSPVDIEKQIAASPMKRVGSPEDIA
                     TACAFLASEQAGYITGQTLGVNGGRVMP"
     gene            complement(1110..1697)
                     /locus_tag="VFX11_12165"
     CDS             complement(1110..1697)
                     /locus_tag="VFX11_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939422.1"
                     /translation="MADIEDKLLTVALQEFQDQGYGAASVTKIVKTAGMSKTTLYSRY
                     PSKEHLFRAILKRQIERYSPWASLGVDSGPLSLEQGLKAYANSTLKLSLENDELAVNH
                     LIYSEIHRFPELGVAAAERTGIGISRIAEFIRKCAVAENIPCKDPDAVAEVFIHMIRG
                     WYINVMLSKRNVSDAQRKQWVERAVRTLLLARADW"
     gene            complement(1902..>3316)
                     /locus_tag="VFX11_12170"
     CDS             complement(1902..>3316)
                     /locus_tag="VFX11_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX4939423.1"
                     /translation="SVFGTALAGGVATTLNTFSTAAELQVLLGNSGCSVLLFERSVLK
                     KDFAAILLDLEPRIAHAVPGELISWAFPFLRHLAMVDSNESAGAIEGWNSFLSRGKDI
                     SPQLVDATAATVSPADPGVLFFSSGSTGKPKGILSAHRGVCLQLWRWRKWLGLDQDVR
                     SLTTNGFFWSGNFAIGVGGTLSAGGTLVLQRTFQPEEALSIMQTERVSYLFAWPHQWA
                     QLVDAKNWRDVDLSALKYIDANSPVASHPTVKSNWIDATHSYGNTETFTLSAAFPANT
                     PREVTGDSHGQPVAGNTLKIVDPLTGQLVPMGERGEIAVKGPTLMLGYIGIPLDEVLD
                     EDGFFHTGDGGYFDDQGRLHWEGRLNDIIKTGGANVSPVEIDTVIAQCPGVKITKTVG
                     VPDELLGEVVVTCIVPLDGFELKESEVRTFAREQLASFKVPRRILFFGEDELQWTGSA
                     KVKSSDLQALATRRLKEQKV"
BASE COUNT          766 a          976 c          852 g          722 t
ORIGIN      
        1 tgttttatat attgaatgat gaaactgcgc gttgctttca tcaaagccgc tgcaaaaacc
       61 gcccgtagaa gccttccagc agatgctcgg cagcgccgga ttttgtggtc atggcaccag
      121 gatcaaggca ttacccgacc accgtttaca cccagcgtct ggcccgtgat gtagcctgcc
      181 tgttccgacg ccagaaatgc gcaggctgtg gcgatatcct caggactacc gacccgcttc
      241 atgggcgacg ccgcgatttg cttttctatg tcgactggtg acttgcgcag catcggggta
      301 tcaacgaacc ccggcgggat gttgtttacc gtaatgcctt ttgcggcaag ctcgagtgcc
      361 agggattttg taaacgcgat gacaccccct ttcgacgcag agtagtgaca catcatcggt
      421 gcaccggttt gggcggacga agaggaaata ttgatgatcc tgccccaacc cgcactcaac
      481 atgtcgggaa tgatttcctg gcagcacagc atcggcccct tgatgttgat ggccatgatg
      541 cggtcaatca tttcttcgga aatatccatg aatggcgtga acgacgagat ccccgcgttg
      601 ttcaccagaa tggtgatggg tccgaacgcc tggcgggtca attgagctcc gtgagcaata
      661 tcggattttg cagaagcatc gccgacacag gcgatagcgc gccctccggc cttggcgatc
      721 atcgcaacgg tttgttccgc actgtcaccg ctcagatccc acaccgatac tgcggctccg
      781 tcctgcgcaa gctgcaggct gatcgcgcgg ccaattccgc taccgccgcc agtaaccaca
      841 gcgaccttgt tttttaatga catggattcc ttctcccaat ggactggcat tgatgggtcg
      901 gcatctgttt cccgacagca tggattaata ataccggtcg gtatttttaa atgtcaagcg
      961 agggaaaatc acaagcagca gggatttccc gcccttttca agcaagggcg gaagttgcgt
     1021 aaagattttg attcagcaaa aagagggaaa tgacaaacgg ggaaaggaag aaagggatcg
     1081 aacgctgact ggctggataa aggcgatgac taccagtctg ctcgcgccag caacaatgtt
     1141 cgtacagcac gctcaaccca ttgtttgcgc tgagcatccg acacattgcg cttggacagc
     1201 atcacgttga tataccagcc cctaatcatg tggataaaca cttccgcaac agcatcggga
     1261 tccttgcagg gaatattctc cgccactgcg catttgcgga taaattcggc tattcgactg
     1321 atgccgatcc ccgttctctc tgcagccgcc acacccagtt ccggaaaacg gtgaatttcg
     1381 ctgtagatca gatggtttac agccagctca tcattttcca gactgagttt cagcgtgctg
     1441 ttggcgtacg ccttcaggcc ctgctccaga ctgagcggac cggaatcgac acccagcgaa
     1501 gcccacggcg aatagcgctc tatctgccgc ttcaggatgg cccggaacaa atgttccttg
     1561 gacgggtatc gcgaatacag cgtggttttt gacatgccgg cggttttgac gatcttcgtc
     1621 accgaagctg cgccgtaacc ctgatcctgg aattcctgga gggccacggt caacagcttg
     1681 tcttcgatgt cggccacatc ctccggtcgg ggacgaccgc gtggacggct cgattccacc
     1741 ttcacggttt ttctgactgc aggtgtttta ctgacggact ttgctgatgc cttggatttc
     1801 atagtatcgg aaaaagtgca attgactggg cgctacagta ccagatttcc cgcagcgctg
     1861 cctattgcac ctgacacttt ggcgtggcca atgacgccga ctcagacctt ctgctccttg
     1921 agccgcctgg tcgccaacgc ctgcaaatcc gagctcttga ccttggcact gcctgtccat
     1981 tgcagctcat cctcgccaaa aaacagaatc cggcgcggca ccttgaagct ggcgagctgc
     2041 tctctggcaa aggtgcgcac ttcactctct ttcaattcaa aaccatccag cggcacgatg
     2101 caggtcacca ccacttcgcc cagcaattca tccggaacgc ccaccgtctt cgttattttt
     2161 accccaggac actgggcaat caccgtatcg atttccactg gcgacacgtt ggccccaccc
     2221 gtcttgataa tgtcattgag acgtccctcc caatgcaggc gaccctggtc atcgaaataa
     2281 ccgccatcgc ccgtgtggaa gaagccgtct tcatccagca cttcatccag ggggataccg
     2341 atatagccaa gcatgagcgt cgggcccttg actgcaatct ctccacgctc gcccataggc
     2401 accagctgac ccgtaagggg gtccacaatc ttgagggtat tgcccgccac aggctggcca
     2461 tgactgtcac cggtcacttc ccgcggtgta ttggcgggaa atgctgcgct cagcgtgaaa
     2521 gtctccgtat ttccatacga gtgggtggca tcgatccaat ttgactttac tgtaggatga
     2581 ctggcaaccg gggaattggc gtcgatgtat ttcagtgcag acagatcgac atcacgccag
     2641 ttcttcgcat caaccagctg cgcccattga tgcggccacg cgaacagata ggacacgcgc
     2701 tctgtctgca taatggacag cgcttcttcc ggctgaaacg ttcgctgcag aaccagcgtg
     2761 ccgccggcag acagtgttcc accaacacca atggcaaaat tgcctgacca gaaaaatcca
     2821 ttcgtggtga gcgaccggac atcctgatcc agacccagcc atttacgcca gcgccacaac
     2881 tgcagacaaa caccgcggtg cgcgctgaga attcctttgg gtttaccggt ggaaccggac
     2941 gaaaaaaaca gcacacccgg atcggcaggc gataccgtag cagcagtggc atcaaccagc
     3001 tggggtgaaa tatcctttcc gcgggacagg aaactgttcc aaccctcaat cgctccggcg
     3061 gattcgttac tgtccaccat ggcaagatgg cgcaagaaag gaaacgccca ggaaatcagt
     3121 tcacccggca ctgcatgggc aatcctcggc tccagatcca gcaggatggc cgcaaaatcc
     3181 ttcttcagaa cgctacgctc aaacaacagt acggagcagc ccgaatttcc cagcagaacc
     3241 tgcaactccg cagccgtgga aaaggtattg agggtagtgg ccacgccgcc ggccaacgct
     3301 gtaccaaaca ctgacg
//
LOCUS       DASUEG010000646         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1596627, whole genome shotgun sequence.
ACCESSION   DASUEG010000646 DASUEG010000000
VERSION     DASUEG010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596627"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFX11_12175"
     CDS             complement(<1..379)
                     /locus_tag="VFX11_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939424.1"
                     /translation="MKSTPNTMHIVLLAITALALPGATLASGNNKHSHEHIQADGETA
                     IGKPGVAAGVTRTITVEMNDSMRYTPSDIQVSEGETIRFVVKNSGQLKHELSLGTEHE
                     LLEHLEVMKKYPDMVHDEPNKITL"
     gene            complement(410..1822)
                     /locus_tag="VFX11_12180"
     CDS             complement(410..1822)
                     /locus_tag="VFX11_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX4939425.1"
                     /translation="MSSRREFFKLAGIAGGAVAASTVGRTVMATLPEPVIQTSPDTMA
                     PLVPDSGQPYNPVVTLNGWTLPWRMNNGVKEFHLVAEPVVREMAPGFKAHLWGYNGQS
                     PGPTIEVVEGDRIRIFVTNQLPEHTSIHWHGQRLPNGMDGVSGLTQPAIKPGKTFVYE
                     FIARRPGTFMYHPHADEMTQMAMGMMGFWVTHPKEKHPLIDEVQRDFCFLLNAYDIDP
                     GSYTPKTMTMLDFNLWSWNSRIFPGIDSLNVRLNDKVRIRIGNLTMTNHPIHLHGHEF
                     LVTGTDGGPTPKNSRWYEVTTDIAVGQMRQIEFVADEEGDWAFHCHKSHHTMNAMGHN
                     VPTLIGVDHRGLAGKINSLIPDYMVMGERGMADMTEMEMPIPDNTAPMMTGDGPFGSV
                     EMGGMFSVLKVRREQKPGHYQDPGWYQHPKGTIAHEYTGALAEPARFKSEGGQSMRRV
                     HESSTPVEVQVRKPALHSDH"
     gene            complement(1836..3266)
                     /locus_tag="VFX11_12185"
     CDS             complement(1836..3266)
                     /locus_tag="VFX11_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4939426.1"
                     /translation="MMVPARIILAWPATILTVILLSACASVSPEQNSSRINDEAAAFT
                     EGHLALALTDEERSRRFKTALALLERPLSQTEAVQLALVNSPSMQALLAQGWAESANA
                     AQAGRISNPIFSFEHVTTGDELELSRALSFGLLDLLTLPRRQGIANRLIEQSQLRLTG
                     IVVDQVTQIRQAWVRAVAAQQSLTYAQQVYVSAEASAELARRMQAAGNFNRISRAREQ
                     AFYADAATSLASATHRATAAREALVRLLGLDEAQARTLQLPERLPDLPREPLTPDTIG
                     SQATRGRLDIRLAQTELDAAAKAQGLNSITSLTDIELAIRRDTVFDNVEGTRSNPRGY
                     EVDVRLPIFDWGDMQRNAMNARTLAAANQLEATMRIAGSSLREGYSTYRTAFDIARHY
                     RDEVIPLRKIIADENLLRYNGMIIGVFELLADARDQVTVVMAAIDAEQQFWLADAALQ
                     ATLIGRPTSTTLTATTGAPSASGAGH"
     gene            3750..4430
                     /locus_tag="VFX11_12190"
     CDS             3750..4430
                     /locus_tag="VFX11_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEX4939427.1"
                     /translation="MKILIVEDEPKTGEYLRQGLSEAGFVIELVQNGNDGLHRALQNE
                     YDLVILDVMLPGLNGWEVLQSIRKHGLQMPVLFLTARDQVDDRVKGLEMGADDYLVKP
                     FSFAELLARVRTILRRGRGGIDAKTLSVADLELDLLRRRATRGGRRIDLTAKEFGLLE
                     LLMRRHGEVLPRSLIASQVWDMNFDSDTNVVEVAMRRLRSKVDDEFGQKLIHTVRGMG
                     YVLEEKKT"
BASE COUNT          874 a         1218 c         1338 g         1070 t
ORIGIN      
        1 tcagcgtgat tttgttcggt tcgtcatgca ccatgtccgg atattttttc attacttcaa
       61 gatgttccag cagttcgtgc tccgtgccca ggctcagctc gtgttttagc tggccactat
      121 tttttaccac gaagcgaatg gtctcacctt cggagacctg aatgtcagac ggcgtgtatc
      181 gcatgctgtc gttcatttca acggttatgg tgcgagtcac tccggctgcc acacccggct
      241 tgccaatggc ggtttcccca tcggcctgta tgtgttcgtg cgagtgcttg ttgtttccgc
      301 tggcgagggt ggcgcctggt agcgccagcg cggtgattgc caataaaact atatgcattg
      361 tgttaggcgt ggatttcatg gtgggttctc ccgggttcaa tctgaatggt caatgatcac
      421 tgtgcaacgc aggtttgcga acctgcactt cgactggcgt gctggattca tggacgcgcc
      481 gcatcgactg tcctccctcc gatttgaacc gtgcgggttc tgccagcgca cccgtgtatt
      541 cgtgggcgat agtacccttc gggtgttgat accatcctgg gtcctggtag tgaccgggtt
      601 tttgctcacg acgcaccttg agcacgctga acatgccgcc catctcgacc gagccaaaag
      661 ggccatctcc ggtcatcatg ggagcggtgt tatccgggat gggcatttcc atctcggtca
      721 tgtcggccat gcctcgctca cccatcacca tatagtccgg tatcaggctg ttgatcttcc
      781 cggcgaggcc ccgatgatcc acgccaatca gcgtgggtac gttgtggccc atcgcattca
      841 tggtgtggtg gctcttgtgg cagtgaaagg cccagtcacc ttcttcatcg gcaacaaatt
      901 caatttgacg catttgccca accgcgatgt cggtcgtcac ttcataccag cgcgaatttt
      961 tgggcgttgg tccgccgtcg gtaccggtga ccaggaactc atgaccgtgc aaatgaatgg
     1021 ggtgattggt cattgtcagg ttgccaatgc ggatgcgcac cttgtcattc aggcgcacgt
     1081 tgagactgtc gatgcccggg aatatccggc tgttccagga ccagagattg aagtccagca
     1141 tcgtcatcgt tttgggcgta tagctgcccg gatcgatgtc ataggcgttg agcaggaaac
     1201 agaagtcgcg ctgaacctcg tcaatcaacg ggtgtttttc tttcgggtga gtcacccaga
     1261 atcccatcat gcccatggcc atctgggtca tttcatctgc gtgcgggtgg tacatgaagg
     1321 tgcctggccg ccgcgcgatg aattcataga cgaacgtctt gccgggcttg atggcgggct
     1381 gggtcaaacc tgacacgcca tccataccgt tgggtaaacg ctggccatgc cagtgaatac
     1441 tggtgtgttc cggtagctgg ttggtgacga aaatgcgaat gcggtcgcct tcgaccactt
     1501 caatcgtcgg gccggggctc tgaccgttat agccccacaa atgggccttg aagccgggtg
     1561 ccatttcccg taccacgggt tcggctacca ggtggaattc cttcacacca ttgttcatgc
     1621 gccatggcag ggtccagcca ttgagtgtga ccaccgggtt gtaaggttgc ccgctatcgg
     1681 gaaccagcgg tgccatggtg tcaggtgatg tctgaatcac cggctcgggc agggtagcca
     1741 ttactgtgcg acctacagtg ctcgctgcca cggcgccacc ggcaatgccg gccaatttga
     1801 aaaattcacg tctggaagac atcggttttc ctttctcagt ggcctgcgcc agaagcactg
     1861 ggcgcgccgg tcgttgcggt aagagtggtg ctggtcgggc ggccaatcaa agtagcttgc
     1921 agcgcggcat ccgccagcca gaactgctgt tccgcatcga tggcagccat gaccactgtg
     1981 acctgatcac gggcgtcggc cagcagctca aacacgccaa tgatcattcc gttgtaacgc
     2041 agcaggtttt catcagcgat gatcttgcgc agcggtatca cctcgtcgcg gtagtgccgc
     2101 gcgatgtcga aggcggtgcg gtaggtcgaa taaccttcgc gcagactgga gccggcaatg
     2161 cgcattgttg cctccagctg gtttgcggct gccagcgtgc gtgcattcat ggcattgcgc
     2221 tgcatgtcac cccagtcgaa tattggcagt cgtacatcga cttcataacc gcgcgggttg
     2281 gatcgggttc cttcgacatt atcgaaaaca gtgtcgcggc gtatcgccag ctcaatgtcg
     2341 gtcaagctgg tgatcgagtt caatccttgt gctttcgcgg ctgcatcgag ttctgtttgc
     2401 gccagacgaa tatccagccg gccgcgcgtt gcctgcgaac caatggtatc cggagtcagg
     2461 ggctccctgg gcagatcggg taggcgttca ggcagctgga gcgtccgtgc ctgtgcttcg
     2521 tccagcccca gcaagcgcac cagtgcttcc cgtgccgcgg tggcgcgatg tgttgccgac
     2581 gcgagactgg ttgcagcatc ggcatagaag gcttgctccc gtgcgcggct gatgcgattg
     2641 aagttgccgg cggcctgcat gcggcgagcc agttcggcgc tggcctcggc gctgacatag
     2701 acttgctgcg catatgtgag gctttgttgt gcggcgacgg cgcggaccca tgcctggcgg
     2761 atctgagtga cctgatccac aacgatgcca gtcagacgca actgcgactg ctcgatcagt
     2821 cgattcgcga tgccctggcg tctgggcaac gtcaacagat cgagcagtcc aaaagataac
     2881 gcgcgggaca attcaagttc gtcacccgtg gtcacatgct caaaactgaa gatgggattt
     2941 gaaatcctgc ctgcttgagc cgcattggcc gattcagccc acccctgcgc caacagggct
     3001 tgcatggaag gactgttcac cagcgccagc tgcaccgcct ccgtctgact caatggacgt
     3061 tctaaaagcg ccaatgcggt tttgaaccgc ctggaccgtt cttcgtcggt tagcgcaagc
     3121 gccaaatgtc cttcggtgaa ggcagcggct tcgtcattga tccggctgga gttttgctcc
     3181 ggactgacac tggcgcaagc tgacaacagg atcacggtca gaatcgtcgc tggccaggct
     3241 agaatgatgc gtgcaggcac catcatcctg atcacgggat agccccttta tgattttcga
     3301 cctgagggac agaatccccg ccagagtcgc aggcagattt cccggtcctg atctccctgg
     3361 cataggcacg ccagcccccg atgcaaccaa ccagatcatt ggcttcgcgc cagggctgta
     3421 cgggttcgtc aatgtagacc tggtacccac tcagcgctga ggtgtaggac aattgagcag
     3481 gtagtgccag attggctgag tcttctgctg gttgcgccgt ggcgatcccg gagaacattg
     3541 tcagcagcca gggcagtaga atgccccagg agcagcgatg gctttgattc tgcataggtt
     3601 cctcgcgacg agtcgcaggt tttggtagaa ctattctgaa gcaccgatac agtcgccagg
     3661 ctgtcacgtg gattacattt ttgttatctg gccaatcgcg cctataaatt cgtcaaacta
     3721 ttctgagttc gaacgggtgg agagcaggct tgaaaatctt gatcgttgag gatgaaccca
     3781 aaacgggcga ataccttcgc cagggtttga gcgaggctgg cttcgttatc gaactggtgc
     3841 agaatggtaa cgacggactg catcgtgcgt tgcagaatga atacgacctg gtgatcctgg
     3901 atgtgatgct ccctggtctg aatgggtggg aagtacttca atccattcgc aagcatggct
     3961 tgcagatgcc ggttctgttc ctgacagcac gcgatcaggt ggacgatcgt gtcaaaggtc
     4021 tggagatggg ggccgacgat tacctggtga agcctttctc cttcgcggaa ttgctggcaa
     4081 gggtgcgtac catcttgcga cgtggcagag gcggtatcga cgcaaaaaca ctgagcgtgg
     4141 ccgatctgga actcgatttg cttcggcgtc gcgccacacg cggtggtcgg cggattgatc
     4201 tgacggccaa ggaatttggc ttgcttgaat tgctgatgcg acggcatggc gaggtcttgc
     4261 ctcgctcgct gatcgcctcg caggtatggg atatgaattt cgacagcgat accaatgtgg
     4321 tggaagttgc gatgcgccgt ttgcgatcca aggtggatga tgaatttggc caaaagctca
     4381 ttcacactgt gcgcggtatg gggtatgtgc tggaagagaa aaaaacatga tgctgcgttc
     4441 ccgctccatt acccgacgca tcagcttgtt gctgggagga tctacgctgg tggtgctgct
//
LOCUS       DASUEG010000647         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_489403, whole genome shotgun sequence.
ACCESSION   DASUEG010000647 DASUEG010000000
VERSION     DASUEG010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489403"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFX11_12195"
     CDS             <1..480
                     /locus_tag="VFX11_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939428.1"
                     /translation="NSVLGDLSEDILSTLNLGGVSSLEDFVDLAYEGNLESFLTHFNR
                     GTVQAVQHSIDWRCGVLQLPADRCEMAKAKAVEDAKKYAEGNTGNIPGIGGGDGRSPI
                     DDIDDIINENIDRVQVTEGSAGSFEWFMLALVVLLLVQRRQIRAVVLRPSGRARHPD"
     gene            496..951
                     /locus_tag="VFX11_12200"
     CDS             496..951
                     /locus_tag="VFX11_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939429.1"
                     /translation="MTGLVVRVQSLLSLLRQSPQARVFVAALLSAATWFAWAFWANRH
                     DLDHAWISGLSQGGVSFITTSVGSSLLEFFYRRIGATLAGRIASVALVSGCSLAFMVS
                     VHTLAHTPNLLTTVLPVFAVVLLYCSSYVIGLQKIKNNYENNEVEVVSP"
     gene            917..2377
                     /locus_tag="VFX11_12205"
     CDS             917..2377
                     /locus_tag="VFX11_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939430.1"
                     /translation="MKITKSKWFRPDVPAVLSSALVTSTLIVCSSTASADSAEWSGWV
                     NAQTRYYPQTGESVDETVYPAAAVQLEYSQALGESDQITGSVFLRGDSVDDERNLGDI
                     RELLWLHSGEAYQFRAGVGQVSWGVNELFKVTDLINQKDRSELPFQRKLGQPLASVSF
                     YVGEDLIELYTMHDVRKAWYPGEDGRLRYPILVDNDEAVYDWGATNRWDFAGRWKTRL
                     WDMDLALSHFYGMTRDPYFIVNYDFNNPSLLPVYEKVNQSSVEWQYAWNDLLLKAEYT
                     YQTGSLEQFESAAGGFEYTFGAVFGSAMDVTWYVEGIWDSRDHIYSTLLDHDVGVAAR
                     LALNDARDSNLILGVVADTEYDEMFGTLFWTNTFGESWTLNLTGQYFMANDDRYDPRD
                     YIPFFEQLVQENPASPESIDYLLSLIGDVTVSKPQYDRILEFVEQLQQPDYWSTVDVA
                     TVPQTMFDLLRISDNSQKMNLIERDSYVQVDVYYHF"
     gene            2393..3172
                     /locus_tag="VFX11_12210"
     CDS             2393..3172
                     /locus_tag="VFX11_12210"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX4939431.1"
                     /translation="MSRILIAALLLLTATFSHAEAPLTGKDIIERAKQNASGFQDMTH
                     RVRMVLVDEKGGKSEREMLLKGLVEKDGTSHSLSIFTAPQREKGIALLSEAKAGEADQ
                     QWLYLPSTKRLKRITGANRTSSFRGSEFTFEDLSDQNSSQYRFDLINQEACASQTCFV
                     IDRFPGAGMESSYSKTRLWIDTEHYRPIKADFYDQSGKLLKTMEAHDYSLFDNKYWKP
                     ARVVMSNKTNGKATEMLSLELKMNTGLQASEFTELAIRSWR"
     gene            complement(3247..>3754)
                     /locus_tag="VFX11_12215"
     CDS             complement(3247..>3754)
                     /locus_tag="VFX11_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939432.1"
                     /translation="HNEFVVSKQLAQRELLLANKGIARMAEQDFLKSLPSINLNYLPK
                     KLRLILMQSVGAFPSLETAARKLGMSGRTLRRQLNSLGTNFQNELDLLRREFAISYLT
                     KSDKCITEIALMLGFCDSSAFSKAFKKWTGESPREFKKNHALDQFREFLGDGEPTQLQ
                     DAELVSPV"
BASE COUNT          793 a         1076 c         1082 g          803 t
ORIGIN      
        1 aacagtgtcc tgggcgacct gagtgaggac atcctgtcca ctctcaactt gggcggcgtt
       61 tccagcctgg aagactttgt tgatctggcg tacgaaggca atctcgagtc attcctgacc
      121 cacttcaatc gtggcacggt acaggcggtc cagcattcca tcgactggcg ctgcggcgtc
      181 ctgcagttgc ctgccgaccg ctgcgagatg gccaaggcca aggcagttga agacgccaag
      241 aaatatgccg agggcaatac gggcaacatt ccgggcattg gcgggggcga tggcagaagt
      301 cccattgacg atatcgatga catcatcaat gaaaacatcg accgggtgca ggtgaccgaa
      361 ggcagtgccg gctcgttcga atggttcatg ctggcgctgg tcgtgcttct gctggtccag
      421 cgtcgtcaga tccgtgctgt tgtcctccgg ccgtccggcc gggcgcggca tcccgactga
      481 tccggtccgc ccttcatgac cggtttggtt gtccgcgttc aatccctgct gagtctgctc
      541 cggcagtcac ctcaggcccg ggtgtttgta gccgcgctcc tgtcggcagc cacctggttt
      601 gcctgggcct tctgggccaa tcgtcacgat ctggatcatg cctggatttc cgggctgtcc
      661 cagggcggtg tcagcttcat caccacctcg gtgggcagca gtctgctgga atttttctac
      721 cggcgtatcg gcgcaacgct cgcgggccgc attgcctcgg tagccctggt gagcggctgt
      781 tccctcgctt tcatggtcag cgtccacacg ctggcccaca cccccaacct gctcaccacg
      841 gttctcccgg tgttcgcggt tgtcctgctg tactgcagca gttacgtgat tggcctccaa
      901 aaaataaaaa acaattatga aaataacgaa gtcgaagtgg tttcgccctg atgtacctgc
      961 tgttctttcc agcgcccttg tcaccagcac actgatcgtc tgctccagca ccgccagcgc
     1021 cgattccgcc gaatggtcgg gttgggtgaa tgcgcagacc cgctactatc cccagaccgg
     1081 tgaaagtgtc gatgaaaccg tttacccggc agccgctgtc cagctggaat acagccaggc
     1141 cctgggtgaa tccgaccaga tcaccggcag cgttttcctg cggggcgaca gcgtcgacga
     1201 cgagcgcaac ctcggtgata tccgcgaact gctctggctg cattccggcg aggcgtacca
     1261 gttccgcgcc ggtgtcggcc aggtgagctg gggcgtcaac gagctgttca aggtgacaga
     1321 cctgatcaac cagaaggacc gctccgagct gccgttccag cgcaagctgg ggcagccgct
     1381 ggcctccgtg tcattttatg tgggcgagga tctgatcgag ctgtacacga tgcatgacgt
     1441 gcgcaaggcc tggtacccgg gagaggacgg ccgtctgcgt tatccgatcc tggtggacaa
     1501 tgatgaggcg gtctacgact ggggtgccac caatcgctgg gatttcgcag gacgctggaa
     1561 aacccggctg tgggacatgg acctggcact gtcgcatttc tatggaatga cccgcgatcc
     1621 ctatttcatt gtcaattacg atttcaacaa cccctccttg ctgccggttt acgaaaaggt
     1681 gaaccagtcg tccgtggaat ggcagtacgc ctggaacgat ctgctgctga aagccgaata
     1741 cacctatcag accgggtccc tggagcagtt tgagtcggcg gcgggcggtt tcgaatatac
     1801 cttcggcgcc gtgtttggtt ctgcgatgga tgtgacctgg tatgtggaag ggatctggga
     1861 tagccgcgat catatctaca gcaccctgct cgatcatgat gtgggtgtgg cggcgcggct
     1921 ggcgctgaac gatgcgcggg acagcaacct gatcctgggt gtggtggcag acaccgagta
     1981 cgacgaaatg ttcggcactc tgttctggac caacacattc ggggaaagct ggaccctgaa
     2041 tctgacaggc cagtacttca tggccaatga tgaccgctat gatccgcgcg attacattcc
     2101 gttttttgaa cagctcgtgc aggaaaatcc tgcctcgcct gaatccattg attatctgct
     2161 cagcctgatt ggcgatgtca cagtcagcaa gccgcaatat gaccggattc tggagttcgt
     2221 cgaacagttg cagcagccgg attactggtc aaccgttgat gttgcaacgg tgccccagac
     2281 gatgtttgat ctgttaagga tttcagacaa ctcacaaaaa atgaacctga tcgaaaggga
     2341 tagctacgta caggtcgatg tgtattacca cttttaatcg ggagaattgt tcatgtcacg
     2401 aattttgatc gccgcgctgc ttctgctgac ggctaccttc agtcacgccg aagcgccgct
     2461 cacgggcaag gacatcatcg agcgcgccaa gcagaatgcc agcggtttcc aggacatgac
     2521 ccatcgcgtg cggatggtgc tggtggatga aaaaggcggc aaaagcgagc gggaaatgct
     2581 gctgaaaggg ctggtggaaa aagatggcac ctcgcacagc ctgtcgatct tcacggcgcc
     2641 acagcgcgag aaaggcattg ctctcctgag cgaagccaag gccggcgaag cagaccagca
     2701 gtggctgtac ctgccgtcca ccaagcggct caagcgcatc acgggcgcaa accgtacctc
     2761 gtcgttccgg ggcagtgaat tcaccttcga ggatctgtcg gaccagaaca gcagccagta
     2821 ccgtttcgac ctgatcaacc aggaagcctg tgccagccag acctgcttcg tgatcgaccg
     2881 tttccccggg gcgggcatgg aaagcagcta cagcaagacc cgcctctgga tcgacacgga
     2941 acactaccgg ccgatcaagg cggatttcta cgaccagtcc ggcaaattgc tgaaaaccat
     3001 ggaagcccat gattacagcc tgttcgacaa caaatactgg aagccggcgc gggtggtgat
     3061 gagcaacaag accaacggca aggccactga aatgctgtcg ctcgaactga agatgaatac
     3121 cggcctgcag gcatcggaat ttaccgagct ggcgatccgg tcctggcgct gaagggcaaa
     3181 agtcagcgct ggcgattcca gcctgcagct gacacaacct gacgggaacg ctacgtcccg
     3241 tcaggatcac accggcgaga cgagctccgc atcctgcaat tgcgtgggct cgccatcacc
     3301 aagaaactca cggaactgat ccagtgcgtg atttttcttg aattccctgg gggattcccc
     3361 cgtccacttc ttgaatgcct tgctgaacgc cgatgaatcg cagaagccga gcatcagcgc
     3421 aatttcggta atgcacttgt cgctcttggt cagatagctg atcgcgaact cccgccgcag
     3481 cagatccagt tcgttctgga aattggttcc caggctgttg agctggcgcc gcagcgtgcg
     3541 gccactcatt cccagtttgc gggcagcggt ttccagcgat ggaaacgcac ccacactctg
     3601 catcaggatc agacgcagtt ttttcggcag ataattcaga ttgatcgatg gcagtgattt
     3661 cagaaaatcc tgttcggcca tgcgcgcgat tcccttattg gcaagcaaca gttcccgttg
     3721 tgccagttgc ttcgacacca cgaattcatt gtgt
//
LOCUS       DASUEG010000648         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1879893, whole genome shotgun sequence.
ACCESSION   DASUEG010000648 DASUEG010000000
VERSION     DASUEG010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879893"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFX11_12220"
     CDS             complement(<1..601)
                     /locus_tag="VFX11_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX4939433.1"
                     /translation="MLNLIWAAFFLIAVVAGFLRFLDGDTAIFQQLVQATFDSAKLSV
                     DIALGLIGVLALWSGLFRIAEKSGLIQKVGQWLSPVFERLMPEVPKGHPAQGAVTMNL
                     AANMLGLDNAATPLGLKAMQELQSLNPSHDTASNAQILFLVLNTASVTLLPITIFMYR
                     AQLGATDPTVVFLPILLASIPSTLVGLIVVMMIQKIRLDA"
     gene            complement(852..1871)
                     /gene="ttcA"
                     /locus_tag="VFX11_12225"
     CDS             complement(852..1871)
                     /gene="ttcA"
                     /locus_tag="VFX11_12225"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX4939434.1"
                     /translation="MSEISASGTFKPEIFQPVSPLDAGVAAVPERLLQAGEEADAEGD
                     DEVDDSVSDDTSPVAIAKKAKTNFNKLQKRLRREVGQAIADFNMIEEGDKIMVCLSGG
                     KDSYTMLDILRSLQRDAPVNFELFAVNLDQKQPGFPEHVIPEYLKKEGVPFHIIEEDT
                     YSVVKSIIPEGKTTCSLCSRLRRGNLYTYAEEIGATKIALGHHRDDIMETLFLNMFYG
                     GKLKSMPPKLLSDNGKHVVIRPLAYCREKDIIRFAEYKQFPIIPCNLCGSQENLQRNA
                     IKAMLQEWDRKFPGRLETIFSAVQNVVPSHLADTELFDFKNLTQMTEQERAQAGMKLD
                     LVNLF"
     gene            2210..>3196
                     /locus_tag="VFX11_12230"
     CDS             2210..>3196
                     /locus_tag="VFX11_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX4939435.1"
                     /translation="MSTIDRRKFLSFVAAGIATPYVVTFTSGSAKAGLIRSDAPLNTD
                     SARVFSLSVASGDPSPTGVVLWTRIDPSAHRSGQALFVQVAHDPDFQQLAFESVVESQ
                     HIKADRDFTVSIDTDGYLEPSIRYYYRFIYDNTVSRTARCRTAPPYGAAIDKLKLGLL
                     TCQDYTNGYYGALNHVANDDSLDFVLHLGDFIYETAGDPRFQSLPFDDRRIILPSDGI
                     VALDLADYRHLYKTYRSDPFLQKAMEQHTFIITRDDHETANDVYWDYERDTLGAPDHP
                     YTVDAAYGNDPYLLNQLMLDSQQAWLEYIPARVHVNPDSTHPHHYLKYYRRVQ"
BASE COUNT          701 a          979 c          837 g          679 t
ORIGIN      
        1 ccgcgtccag acgaattttc tggatcatca tcaccacgat caggcccacc agggtggagg
       61 gaatggacgc cagcagaata ggaaggaata cgacggtggg atcggtggca cccagctggg
      121 cgcggtacat gaaaatcgtg atgggtaata acgtgaccga tgcagtattc agtaccagaa
      181 acaggatttg tgcgttactg gcggtatcgt gcgacggatt caggctttgc agttcctgca
      241 tcgccttcag gcccagcggc gtggcggcgt tatccagccc cagcatgttg gcagcgaggt
      301 tcatggtcac cgcgccctgg gccgggtgtc ctttcggcac ttccggcatc aggcgttcaa
      361 acacggggct cagccactgc cctactttct ggatcagtcc acttttttcg gcaatacgaa
      421 acaggcccga ccagagtgcc agcacaccga tcagccccag cgcaatgtcc accgacagtt
      481 tggcggaatc aaaggtggcc tgaaccagtt gctggaaaat ggcggtgtcg ccgtccagaa
      541 agcgcaaaaa tcctgctacg acggcgatga gaaaaaatgc agcccaaatc agattgagca
      601 aggcgatggt ccttcaagaa attggcgtaa ctggcgcgaa cgcagaggaa gcgggctatt
      661 taacgagaat cgccacgagt acatccctgt aggctcgaca gcggcccctt cgggtccagc
      721 cactcacgaa tacgttcgcg gcgttcgcga gggaaatgca tgcatttcca tatcccgctc
      781 acccatggcc gctgacgttc tcgtaatata gcccgcttcc tctgctaccc atcatccctg
      841 gcacgtcatg gttaaaacaa gttcaccaga tccagcttca ttcccgcctg tgcccgctcc
      901 tgttccgtca tctgcgtcag gtttttgaaa tcgaacagct ccgtatccgc cagatgcgac
      961 ggcaccacat tctgcacggc ggaaaaaatc gtctccagac ggccggggaa tttcctgtcc
     1021 cattcctgca gcatggcctt gatcgcattg cgctgcaggt tttcctgcga cccgcacaga
     1081 ttgcacggaa tgatcggaaa ctgcttgtac tccgcaaacc ggatgatgtc tttctcacga
     1141 caatatgcca gcggacggat caccacatgc ttgccgttgt cgctcaacag tttcggcggc
     1201 atggatttca gtttgccgcc ataaaacatg ttgagaaaca gggtttccat gatgtcatcg
     1261 cgatgatgcc ccagcgcaat cttggtggcg ccgatttctt ccgcatacgt gtaaagatta
     1321 ccgcgacgca gacgggaaca cagcgagcag gtggtcttgc cctccgggat gatgctcttc
     1381 accacgctgt aggtgtcttc ctcgatgata tggaacggca caccttcttt tttcagatac
     1441 tcgggaatca cgtgctcggg aaagcccggc tgcttctggt ccaggttcac ggcgaacagt
     1501 tcgaagttca ccggcgcatc gcgctgcagg ctgcgcagga tatccagcat ggtgtaggaa
     1561 tccttgccgc cggacaggca caccatgatc ttgtcgcctt cctcgatcat gttgaagtcg
     1621 gcaatggcct gacccacttc ccgccgcagg cgtttctgca gcttgttgaa gttggtcttg
     1681 gcttttttgg cgatggcgac agggctggtg tcgtcggaaa cggagtcatc gacctcgtcg
     1741 tcaccctctg catcggcctc ttcacccgcc tgcagcaacc gctcgggaac tgcagcaaca
     1801 cccgcatcca gcgggctaac cggctggaaa atctcgggtt taaaggttcc ggacgcactg
     1861 atctcggaca tgggagacaa cctctggacg acaggcaaac gtggggggct ttgtacccga
     1921 ttccccgtag cttgtaaact gagcgaacgg tatgctggaa agtgtggctg atgagtctgc
     1981 cagcgacatt gcaccttcgt accacaacga ttagagtatg atcaaagtcg agcctgaaca
     2041 ggttcacttt tttcgcgact gaaatactaa ctacacaata aaaagctaaa aaacgcactt
     2101 ccgaacccgg ggctccccgc cctaccctcc catagtcggg actgaaatca ggttcggatt
     2161 ttggctaaac gcagttttag cttgaacaaa ggatgtcact ggagccccca tgagcacgat
     2221 tgaccgccgc aagttcctct cgttcgttgc cgcaggaatc gctacaccct acgtcgtcac
     2281 ctttacctcc ggctccgcca aagcgggcct gatccggtct gacgcgccac tcaacaccga
     2341 ttccgcccgc gtgttcagtc tgtcggtggc atccggcgat cccagcccca ccggcgtcgt
     2401 gctctggacc cgcattgacc ccagcgcgca ccgcagcggc caggccctgt tcgtccaggt
     2461 agctcatgat cccgacttcc agcaactggc ctttgaaagc gtggtggaaa gccagcacat
     2521 caaggcggac cgcgatttca ccgtgtcgat cgacaccgac ggctacctgg aaccgtccat
     2581 ccgctattac taccgcttta tttacgacaa caccgtgagc cgcaccgccc gctgccgcac
     2641 cgcgcccccc tacggtgccg cgatcgacaa gctgaaactg ggcctgctca cctgccagga
     2701 ttacaccaac ggctactatg gcgcgctgaa tcacgtggcc aacgacgatt cactggattt
     2761 cgtgctgcac ctgggagatt tcatttacga aaccgccggc gacccgcgct tccagagcct
     2821 gcccttcgac gaccgtcgca ttatcctgcc cagcgacggc atcgtggcac tggatctggc
     2881 ggattaccgc catctgtaca aaacctatcg cagtgatccg ttcctgcaaa aggcgatgga
     2941 gcagcatacc ttcatcatta cccgcgacga tcacgaaact gctaatgatg tgtactggga
     3001 ttacgagcgc gataccttgg gcgctccgga ccacccctac acggtggatg cggcctatgg
     3061 caatgatccc tacctgctga atcaactgat gctggattcg cagcaggcct ggctggaata
     3121 cattcccgcc cgcgtgcacg taaatcccga cagcacccat ccgcatcatt acctgaaata
     3181 ttaccgtcgc gtgcaa
//
LOCUS       DASUEG010000649         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1483621, whole genome shotgun sequence.
ACCESSION   DASUEG010000649 DASUEG010000000
VERSION     DASUEG010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483621"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFX11_12235"
     CDS             <1..803
                     /locus_tag="VFX11_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939436.1"
                     /translation="RSLYQPDTGSSPTNNAASNNDRVVAWYWRHGDKPRPTIVLVHGF
                     LAPWWEINEFYLGLRFLYDLGCDVVLKTLPHHGPRAHSAKRVSGMGYFSRGVDSLNHA
                     VVQSTYDIRTLVDVLQQRGVNKIGLSGVSLGGYTTALLAGLDERFEFVMPLVPLISIP
                     DAIMEWKPLDRIAKTFMRRYGVDMRDLRNTMAFHSPLSRPALLPPDRLLIIAGMGDRL
                     ASPRHAETLQQHWGNCDLHWYAGAHALPRQQYQTNLVKQAFLTRIGFI"
     gene            831..1802
                     /locus_tag="VFX11_12240"
     CDS             831..1802
                     /locus_tag="VFX11_12240"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939437.1"
                     /translation="MSRSVLVTGAFGNIGSRVVRHLLGAGHRVVAVDLKTPKTEALAA
                     TFGDAIEPIWGNICDPTLWPRALTGVDAVVHMAAIIPPAADRNPELTLAVNQTATLEL
                     LKSMELSPTAKRLVFASSMTVAGHEQDRRTPPLKVEDEMQPTDLYGETKAECERRIRA
                     SSLRWSILRVAACPPTEMSFSGGAASIDVIFETSPNGRVEVVHNDDAALAFANAVECD
                     AAIGKILFVGGGEKCQTYALDFYNRALGATGLGPLRASAFRPGPPYFYGDWLDTAESQ
                     RLLAFQLHSIDDIVADLKASAGFKRQLLKLVSPLLNRMLERRSPYRP"
     gene            complement(1815..2015)
                     /locus_tag="VFX11_12245"
     CDS             complement(1815..2015)
                     /locus_tag="VFX11_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939438.1"
                     /translation="MRTRFALLAIITGRDEIVPNAHSHNLHKAWKGPKQLQMIDAAGH
                     NDVTAFPDCQRAILEFLQARAV"
     gene            complement(2114..2551)
                     /locus_tag="VFX11_12250"
     CDS             complement(2114..2551)
                     /locus_tag="VFX11_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4939439.1"
                     /translation="MRYLNAAFMESQRLTPTIMATMRCLIKDTHVAGYNLPAGVSIVT
                     SPYLIGRRKDVWGDDSEIYRPERWLENKGPKPYEFFPFGGGQRTCVGLNQARQQLKIF
                     FAVLLLKTRWESKYKDNDIWPSQTNIAGTTQPAGGVPITIRRK"
     gene            complement(2585..>3094)
                     /locus_tag="VFX11_12255"
     CDS             complement(2585..>3094)
                     /locus_tag="VFX11_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939440.1"
                     /translation="NSERLKDRAVDMYDIIERRIEQLQDGQPIDMTRFVGDVTLDIAC
                     FSLTGHKSGPVKDRYKNLMAKWLHLSTNNTMFMLGMMMGATRWRHRMHRLYQDKIAKG
                     NVGKEKRLYLTPWGRAVELKAQLDQLLRNDIRHARANRDDNRNDILFYLSIATDSSGE
                     LLSEDRVIA"
BASE COUNT          589 a          836 c          945 g          724 t
ORIGIN      
        1 ggcggtctct gtatcagccg gataccggct cttctcccac caataatgcc gcctccaata
       61 atgacagggt ggtggcctgg tactggcggc atggggacaa gccccgcccc acgattgtgc
      121 tggtgcatgg tttcctggcg ccctggtggg aaatcaacga attctatctg ggtctgcgtt
      181 tcctctatga cctgggttgc gacgtggtgc tgaagacgct gccgcatcat ggcccccgcg
      241 cccattcggc aaaacgggtg agcgggatgg gctatttctc ccgtggcgtg gactcattga
      301 atcatgcggt ggtgcagagc acctacgata ttcgcacgct ggtggatgtg ctgcagcagc
      361 gcggtgtcaa caagatcggg ttgagtggtg tcagtctggg ggggtatacc accgcactgc
      421 tggcggggct ggatgagcgc tttgaatttg tgatgccgct ggtgccgttg atcagcattc
      481 ccgatgccat catggagtgg aagccgctgg accggatcgc aaagaccttc atgcgccgtt
      541 acggcgtcga catgcgggat ctgcgtaata ccatggcgtt tcacagcccc ttgtccaggc
      601 cggcattgtt gccgccggac cgcctgctga ttatcgcggg catgggtgat cgtctggcct
      661 cgccccgtca cgccgaaacc ctgcaacagc attggggcaa ctgtgacctg cactggtacg
      721 ccggtgcgca cgcgcttcca cggcagcaat atcagaccaa tctggtgaaa caggcttttc
      781 tgacccgtat cgggtttatc tagcgttcag tagcaatttc tgggggttgt atgtcgcgtt
      841 ctgttctggt gaccggtgca ttcggaaata tcggtagccg cgtggttcgt catttgctgg
      901 gcgcgggtca tcgtgtggtg gcggtggatt tgaaaacacc gaaaacggaa gcgcttgccg
      961 ctacattcgg tgacgcaata gaacccatct ggggcaacat ctgcgatccg accctttggc
     1021 cgcgcgcgtt gacgggcgtg gatgctgtcg ttcacatggc ggcgattatt ccacccgctg
     1081 cggatcgcaa tccggaactt acgctcgctg tgaatcagac cgcgacactg gaattgctga
     1141 aaagcatgga gctgtcgccc actgcaaaac gcctggtgtt tgcatcgagc atgactgtgg
     1201 cgggacatga gcaggatcgg cgtacgccac cactcaaggt ggaagatgaa atgcagccga
     1261 cggatctgta tggagaaacc aaggccgagt gtgaacgccg cattcgtgca agttcgttgc
     1321 gctggtcgat cctgcgtgtt gccgcctgtc cgccaaccga aatgtcgttt agcggcggcg
     1381 ctgcctcgat tgatgtgata ttcgagacca gccccaacgg ccgtgtcgag gtggtacaca
     1441 acgatgatgc cgcgctcgcc tttgcgaatg cggtggagtg cgatgcggcg attggcaaga
     1501 tcctgtttgt tggcgggggt gaaaaatgtc agacctatgc gctcgatttt tacaatcgcg
     1561 cgctgggcgc gacaggattg gggccgctga gagcaagcgc atttcgtccc gggccgccgt
     1621 atttttatgg cgactggctc gacacggcgg aaagccagcg actgctggcg tttcagcttc
     1681 acagcatcga cgacatcgtt gccgacctca aagcaagcgc gggttttaaa cggcagctgc
     1741 tgaaactggt ttccccgctg ctgaatcgca tgctggagcg acgctcaccg tatcggccgt
     1801 aacggcgttc tggatcagac ggcgcgcgcc tgcaaaaact ccagtatcgc ccgctggcaa
     1861 tccggaaaag cagtgacgtc attgtgaccc gccgcgtcaa tcatctgcag ctgtttgggc
     1921 cctttccagg ccttgtgcag gttgtgtgag tgggcgttgg gaacgatctc gtcccgcccg
     1981 gtgatgatgg cgagcagtgc aaatcgcgtt cgcaattccc tgttttccga tgacggctgt
     2041 tgagggagac cgccattgtt gatgaaagcc cgccaggatg ctgtgacacc tgccgggcaa
     2101 ttgtgtgaac aatctacttt cgccggattg tgatgggcac gccaccggcc ggctgcgtgg
     2161 taccggcaat attggtttgg ctgggccaga tgtcattgtc tttgtatttc gattcccacc
     2221 tggttttaag caatagcacg gcaaagaata tcttcagttg ctgccgggcc tgattcaagc
     2281 cgacacaggt gcgctggccg ccgccaaacg gaaaaaactc atagggcttt ggccccttgt
     2341 tttccagcca tcgttccggt cgatagattt ctgaatcgtc cccccagaca tccttgcggc
     2401 gcccgatcag atagggcgac gttacgatac tgacgccagc cggcagattg taacctgcaa
     2461 cgtgggtgtc cttgatcagg cagcgcatgg tggccatgat ggtgggtgtc aggcgctggg
     2521 attccataaa ggcggcattc agatagcgca gctggctgac ggtgtaggca ttgaagccgc
     2581 cgtgctaggc gatgaccctg tcttctgaca gtaattcacc gctgctgtcg gtggcgatgc
     2641 tgaggtaaaa cagaatatcg tttctgttgt cgtcccgatt cgcgcgcgcg tgccggatat
     2701 cgttacgcaa cagttgatcc agctgtgcct tcagttcaac ggctctgccc cagggtgtta
     2761 gataaagtcg cttttccttg ccgacattgc ctttggctat tttgtcctgg tagagccggt
     2821 gcatgcgatg acgccagcgg gtggcgccca tcatcatgcc cagcatgaac atggtgttgt
     2881 tggtggaaag atgcagccat ttggccatca ggtttttgta gcgatctttc accggtcccg
     2941 acttgtggcc ggtcagtgag aagcaggcga tatcgagtgt cacatcgccg acgaagcggg
     3001 tcatgtcaat gggctggcca tcttgcagct gttcaatcct gcgctcgata atgtcgtaca
     3061 tatcgacggc gcgatctttc agccgctcag aatt
//
LOCUS       DASUEG010000650         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_423369, whole genome shotgun sequence.
ACCESSION   DASUEG010000650 DASUEG010000000
VERSION     DASUEG010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423369"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFX11_12260"
     CDS             <1..585
                     /locus_tag="VFX11_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939441.1"
                     /translation="YMPMPGLKNRVDVGPVCRVGRKLDLHAHFGHEPDVRHALVTMGG
                     MPYPLPFERWPQRSDLFFIHAGVPTTPRADLINLADCGISHLDLVASCAVILTKPGYG
                     TFMEAACSGTPVLYVSRGDWPEQPPLLAWQRQHGYCREITETHFQQGYFVDDIEQVVV
                     AARSAQYAERVSPTGIDEIIARIELQLGKSGGDT"
     gene            1003..1716
                     /locus_tag="VFX11_12265"
     CDS             1003..1716
                     /locus_tag="VFX11_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEX4939442.1"
                     /translation="MNTRSLLDQLFQSGSELLARQSGVSRPSGGASGAPTGGNDPLSG
                     FVSGAAGGALAAGAMNLLMGNKKARKVSGNLAMYGGLAALGVVAYKAYDNWQKNQPGM
                     VMHEPRTIDRLGGAQAEEHSQAILRALIGAAKADGHIDERERKLIDAEISKIGGDPQL
                     VRWFDQELRKPLDPADVAAAARSPEMAAEMYIASVLVVDEDSYMERVYLQELSRQLKL
                     DPSLVSELEQQVAQAAPRA"
     gene            complement(1779..2141)
                     /locus_tag="VFX11_12270"
     CDS             complement(1779..2141)
                     /locus_tag="VFX11_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX4939443.1"
                     /translation="MTHYVDGFVLPVPKKNLDAYRKMAQLAGTVWREHGALEYVEWVA
                     EDVKPGTLTSFPQAVKLEEGETVIFAWIRYESRARRDEINAKVMEDPRIKDAFDPNTM
                     PFDGKRMFWGGFEKLVEI"
BASE COUNT          437 a          610 c          683 g          461 t
ORIGIN      
        1 tacatgccca tgccgggttt gaaaaaccgg gtggatgtgg ggcccgtgtg cagggtgggt
       61 cgaaagctgg atcttcacgc ccatttcggg catgagcctg acgtgcgcca tgcgctggtg
      121 accatgggtg gcatgcccta tccgttgcca ttcgagcgct ggccacagcg cagcgatctt
      181 tttttcatcc atgcgggtgt acccaccact ccacgggctg atctgatcaa tctggctgac
      241 tgcggtatat cccatctgga tctggtggcc agttgcgcgg tgatactcac caagccgggt
      301 tacggcacgt tcatggaagc cgcctgcagc ggcacgccgg tgctctatgt gtcccggggc
      361 gactggcccg agcagccccc gctgctggca tggcagcggc agcacgggta ttgccgggag
      421 atcacggaaa cgcacttcca gcagggctat tttgtggacg acatcgagca ggtcgttgtg
      481 gcagcccgca gtgcacagta tgcggagcgg gtttccccca cgggaattga tgaaattatt
      541 gcccggattg aactgcagct gggtaaaagc ggcggggata cgtagaacgc tcgggccgga
      601 aagaaaaaag ggcggaagga aaaataaaac gccagaacgc cgttacggtc gtcctggcga
      661 gtcgtgccca ggaaaagcag caccaaccaa catcactctt tcaacttcat tcaacgcggg
      721 gaatgccggc gccgtacagg ggcggcgccg gcgagaaaca ggtcatgagc caaatcctgt
      781 ttcctaatcc catagtccgt aggcgcgcaa acaaacagga attttcggcg caaaaaaatg
      841 gcattttggt attaatgcac atttttgctg gtgctaagag tctgactggg ggaacctgtg
      901 ggcgatattg gaggtctcct gtcagatgtc tgcacagacg gttatcaggt tgtgatagcc
      961 ggcctttatc cccctcatct gcaccattcg gagagtcttg ccatgaatac ccgcagcttg
     1021 ttggatcagc ttttccagtc cggcagtgaa ctacttgcca ggcagtccgg cgtttccaga
     1081 ccgtccggcg gtgcgtctgg cgctccgacg ggcggtaatg atcccctgtc cggctttgtg
     1141 tccggcgctg ccggcggcgc tttggcggcc ggcgccatga atctgctgat gggcaacaag
     1201 aaggcgcgca aggtcagtgg caacctggcg atgtacggcg ggctggcggc gctgggtgtg
     1261 gttgcctaca aggcgtatga caactggcag aaaaaccagc ccggcatggt catgcatgaa
     1321 ccgcgcacga tcgaccgcct tggcggcgca caggccgagg agcacagcca ggccatcctg
     1381 cgagccctga tcggtgcagc caaggccgac ggacatatcg atgagcgcga gcgcaagttg
     1441 atcgatgccg aaatcagcaa gattgggggc gatccccagc tggtgcgctg gttcgatcag
     1501 gaattgcgca agccgctgga cccggcggac gttgctgctg ctgcccgctc gccggaaatg
     1561 gcagccgaga tgtatatcgc cagcgtgctg gtggtggatg aagacagcta tatggagcga
     1621 gtctatctgc aggaactgtc acgccagctg aaactggatc cctcgctggt gtcggaactg
     1681 gagcagcagg tggcgcaggc cgcaccgcgc gcctgacgag tcgcggtccg gttgcatgca
     1741 aaggctcggg ccagccggtt tcagactggc ccgggcgttc agatttctac caatttttca
     1801 aaaccgcccc agaacatccg cttgccatca aatggcatgg tattcgggtc aaatgcgtcc
     1861 ttgatgcggg gatcctccat taccttggca ttgatttcat cgcgcctggc ccgcgattca
     1921 tatctgatcc aggcgaagat cacggtttca ccttcttcca gtttgactgc ctggggaaac
     1981 gaggtcaggg tgccgggctt cacatcctcg gctacccatt ccacgtattc cagggcgccg
     2041 tgttcgcgcc agacggtgcc tgccagttgc gccatcttgc ggtacgcatc cagattcttt
     2101 ttcggtacgg gcaatacaaa tccgtcgaca tagtgggtca tgttgtgtgc tcctgttgtt
     2161 gcgggggtga gtgggcagtt gcgatatcct t
//
LOCUS       DASUEG010000651         6981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_606867, whole genome shotgun sequence.
ACCESSION   DASUEG010000651 DASUEG010000000
VERSION     DASUEG010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606867"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1455
                     /locus_tag="VFX11_12275"
     CDS             280..1455
                     /locus_tag="VFX11_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939444.1"
                     /translation="METRYSQEQTALRLQLRDYFSGLMTPEKVAACMGKEGGPIFRGL
                     VEQLGRDGMLTLGWSSEYGGKNYTAVEQLILFEEAWKTHTPFPLITLNSVAPSIMAFG
                     TDEQKQFFLPQIAAGKCIFAIGYSEPGSGTDLASLKTKAEFDGENWIINGSKVWTSAG
                     HDCDYIWLAARTSSDGKRPSDGITLFIVDAKDPGYTHAPIHTVAGVDTNVTYYTNVKV
                     PANRVVGKVNGGWKAITSQLNHERIVLAAMTVIARKQFGYVLKRLARPDASGQRRLDD
                     PTVQQQIGELYARLESMEVLNLRNANNVDTHKIDVALASGSKVLNTLEQIRVLRALCE
                     LAGDDAAVQYESADAIFSGHLEYDYRAAQIMTVGGGVLEVMRSMTATFGLGMPNTIV"
     gene            1474..2607
                     /locus_tag="VFX11_12280"
     CDS             1474..2607
                     /locus_tag="VFX11_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939445.1"
                     /translation="MNFNYSEDQLAIKDVADRMFRDLCGDEQIRALFKTAQPFHAELW
                     AQLAGAGLLAATLPAELGGSDMGLLELSLVLEAQGRSVAPVPLLESVVACAMPIARFA
                     PDELKRSLVPTLASGEAILVPVRPYDGLQDFTPLKATATSSGWELNGSSSLCLYAPVA
                     NGFLVQAKLNGSSHWVGYCAANTVGVRIVQQRASSGEIAGQIHFDQVKISAANTLAIA
                     DEAETLLEWQAQRVFVAMAAQQVGILREGMQRAASYTNERTQFGRSLSSFQAVAQQAA
                     DGYMAVEALQGIVWRALEDIDNDADTVALSARVAKFWLCESGHVAAHVFLHLHGGIGQ
                     DLDYPLHRYFIWAKKNEVILGSARQHSVALGKQIAANPELFYG"
     gene            complement(2801..3124)
                     /locus_tag="VFX11_12285"
     CDS             complement(2801..3124)
                     /locus_tag="VFX11_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939446.1"
                     /translation="MTANTAAAIDGAWDFVVKVPTGEQKSVVTLKSQGDKLTGTMNSA
                     EYGVQEIEEGKYDGETLTWKSKTTKPMKLTLTYTATVDANNNLKGFIKVAMAKLQFTG
                     TPIAK"
     gene            complement(3307..4662)
                     /locus_tag="VFX11_12290"
     CDS             complement(3307..4662)
                     /locus_tag="VFX11_12290"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX4939447.1"
                     /translation="MTESDTLAPLRVATAPSRHDVAQLRFHPEREKLFNELHTRPFPV
                     LETGSRVSQFAVLHGEQGADTEYRHIGQLCQRFAVVPPVSGSSCYYQDFGGFELRWER
                     HNEFSTYTFIVRDLLAAPFTCNALTLLPAGWLGGIPGQVIHAQHIDILTAPLELQTAD
                     EQRRHFEGHRLISSWVGDEQARLWTSYRIHNDGFGRILIFNRDLNPCQSGRLVRALLE
                     VETYRMMLLTAFPLARAIAPQVSDMAQQLAIINQQINAIRGLDDERRLLQQLSTLAAR
                     IEQMISDSNYRFSAARAYYDLVISRLKDLREQEVGGMQTISEFLRRRLTPAYRTCQSV
                     SGQLDDLSQRIDRASELLRTRVNLTLEAQNQDLLQSMNKRSKLQLQLQQAVEGLSVAA
                     ISYYMVGLVKYLGEFLKDQGWLEHPALLTGIAVPCSIAVVWWGLRRLRKRIQAPGSAQ
                     G"
     gene            complement(4870..5562)
                     /locus_tag="VFX11_12295"
     CDS             complement(4870..5562)
                     /locus_tag="VFX11_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX4939448.1"
                     /translation="MTSPIDTATLSASFTDIINRRRSVRGFLSTPVPSDTIRTIFTLA
                     QRTPSNCNTQPWLTHVVSGEKLETLRQRMYENAMLGKMTLDYPYSGKYDGVYKERQYD
                     AAVQMYNAVGIAREEKQKRLEVFLRNYRFFNAPHVAFLFLPEPFGIREAADVGMYAQS
                     LMLGMMAFGLGSCPQTSLGFHADLVREVLGIDPANKLLFGISFGYEDTADPINNARLG
                     RAELEATTVFHD"
     gene            complement(5590..6609)
                     /locus_tag="VFX11_12300"
     CDS             complement(5590..6609)
                     /locus_tag="VFX11_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4939449.1"
                     /translation="MGLHYPFTDPPGTGNAVSITPDVKWLRSPLPISLGHINCYLLRD
                     GDGWCVLDTGMNGAAARQQWLDVIETELQGAPLTRVIVTHHHPDHVGLAGWLCDRFRI
                     PLYMSESEYFQSLAFNAPRRSQPYWEVDQYFARAGMSLDSQQSLYANDDYNHMVSEMP
                     ASYHRITDGQRVRIGDFEWQAITTRGHAPEHLSLYCRERNLYISGDQLLPKITSNVSV
                     SPVAPEADPLADWLDAHDKLTAIVPDEATVLPAHQLPFTGVHVRAAQVVHHHQQRMDQ
                     IHTLCQQPATAQVLTEKLFDRKLDAFQNFLAVGECMAHLHRLLALGRVERTLTEGVYH
                     FRALT"
     gene            complement(6613..>6981)
                     /locus_tag="VFX11_12305"
     CDS             complement(6613..>6981)
                     /locus_tag="VFX11_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939450.1"
                     /translation="KALDITLEHVKTRKAFGAPLFEKDIIRDKLARLQTKIALARNYL
                     HYVAWLDAQGADCIKEVSMLKANACEIANEVMYDCVQLHGGMGFMQECVIERMYRDAR
                     VLPIGGGATEVMYGEVAKRL"
BASE COUNT         1413 a         2047 c         2142 g         1379 t
ORIGIN      
        1 gaattcgccg aacgggtgta ccggcaaatg atctgcgcgc ggcggatgaa gctgtcgtat
       61 ctgatcggac ccggcgtggc acccctgccc tggctgcacc gcctgctgcc ggcacaacag
      121 gtgcagaaca tttgggcgaa gatgttcggg cgcaaggcgt aacagcacga tcactattat
      181 gcaatgctat tgcataatag ttgcataatt tgttaccatt catccaactg agagcccaac
      241 ccggcgcttt ccccattcca agccagaacg acgaccacga tggaaactcg atacagccag
      301 gaacaaaccg ccctgcgcct gcagttgcga gactacttca gcggcctgat gacgcctgaa
      361 aaggtggcgg cctgcatggg caaggaaggc ggcccgatct tccgtggcct ggtggaacag
      421 ctgggccgcg acggcatgct gaccctgggc tggtcctctg aatacggcgg caagaactac
      481 accgccgtcg aacagctgat cctgttcgag gaagcctgga aaacccacac accgttcccg
      541 ctgatcaccc ttaactcggt ggccccgtcc atcatggcgt tcggcaccga cgagcagaaa
      601 cagtttttcc tgccccagat cgccgccggc aaatgcatct tcgccatcgg ctactccgaa
      661 cccggctccg gcaccgatct ggcatcgctg aaaaccaaag ccgaattcga cggcgaaaac
      721 tggatcatca acggcagcaa ggtgtggacc agcgccggcc acgattgcga ttacatctgg
      781 ctggcggcac gcaccagcag cgacggcaag cgcccctccg acggcatcac cctgttcatc
      841 gtcgatgcca aggaccccgg ctacacccac gcaccgatcc acaccgtggc gggcgtcgac
      901 accaacgtca cctattacac caacgtgaaa gtgccagcca atcgcgtggt gggcaaagtc
      961 aacggcggct ggaaagccat tacatcgcag ctgaatcacg agcgcatcgt gctggcagcc
     1021 atgaccgtga tcgcgcgcaa gcaattcggc tatgtgctga aaagactggc gcggcccgat
     1081 gccagcggcc aacgccggct cgacgatccc actgtgcagc agcagatcgg cgaactctat
     1141 gcccgcctgg aatccatgga agtgctcaac ctgcgcaacg ccaacaatgt ggacacccac
     1201 aaaatcgacg tggcgctggc atccggctcg aaggtgttga acacgctgga acagatccgc
     1261 gtcctgcggg cactgtgcga actggcgggc gacgatgccg ccgtgcagta cgaaagcgcc
     1321 gatgcgattt tctccggcca tctggaatac gactaccgcg ccgcgcagat catgaccgtg
     1381 ggcggcggtg tgctggaagt gatgcggtcg atgaccgcga cctttggcct gggcatgccc
     1441 aatacgatcg tttgatacag caggagtcga cgcgtgaatt tcaattacag tgaagaccag
     1501 ctggcgatca aggatgttgc cgaccgcatg ttccgcgatc tttgcggcga cgaacagatc
     1561 cgcgcgttat tcaaaactgc gcaaccgttc catgcagaac tgtgggcgca actggccggc
     1621 gccggattgc tggcggccac gctgccggca gaactgggcg gctcggacat gggcctgctg
     1681 gaactgagcc tggtgctgga agcgcaaggc agatccgtcg caccggtgcc gctgctggaa
     1741 tccgtcgtgg cctgtgccat gccgatcgcc cgcttcgcgc cggacgaact gaaacgcagc
     1801 ctggttccga cgctcgcgag cggtgaagcc attctcgtgc cggtccgccc ttacgacggc
     1861 ttgcaggatt tcactccgct gaaagccact gccaccagca gcggctggga actgaacggc
     1921 agcagcagcc tgtgtctgta cgcgccggtg gccaacggtt ttctggtgca ggccaaactc
     1981 aatggcagca gccactgggt gggctattgc gctgccaaca cagtgggcgt ccgcatcgta
     2041 cagcaacgcg ccagcagcgg cgaaattgcc ggccagattc atttcgatca ggtgaaaatc
     2101 agtgccgcca acaccctggc aattgccgat gaagcggaaa cgctgctgga atggcaggca
     2161 caacgcgtgt ttgtggcgat ggcggcgcag caggtgggaa ttttgcgtga aggcatgcag
     2221 cgcgcggcga gctacaccaa cgagcgcacc cagttcggcc gttcattgtc gtcgttccag
     2281 gcggtggcgc aacaggccgc cgacggttac atggcggtgg aagccttgca aggcattgtc
     2341 tggcgcgctc tggaagatat cgacaacgac gcggacaccg ttgccctctc cgcccgcgtc
     2401 gccaagttct ggctgtgcga gtccggtcat gtggccgcac atgtgttcct gcatctgcac
     2461 ggcggcatcg gccaggatct ggattatccg ctgcaccgct attttatctg ggcgaagaaa
     2521 aatgaagtga tcctgggatc ggcgcggcag cacagcgtgg cgcttggcaa acagatcgcg
     2581 gcgaatccgg aattgtttta cggctgaagc ggatcggtgc cagaatcaaa aaagccgcgc
     2641 aatgcggctt ttttaatgtg ccttgttttt tatgtgcccg gtttctttgg actcgtatta
     2701 acagcgagcc ggctttaggt attctgattt gatgctttct gatttcaggc acgctggcaa
     2761 cagcgacagg cgctgcgcca gacggtcaga tcacaaacca ctatttcgcg atcggtgtgc
     2821 cggtgaattg cagtttggcc atcgccactt tgatgaaacc tttcagattg ttattggcat
     2881 ccaccgtggc cgtgtaggtc aacgtcagtt tcatgggctt ggtggttttg cttttccagg
     2941 tgagagtttc gccgtcgtac ttgccttcct cgatttcctg cacgccgtat tctgcgctgt
     3001 tcatggtgcc ggtcagtttg tcgccctggg atttcagggt caccacgctt ttctgttcgc
     3061 cggtgggaac cttcaccaca aaatcccatg cgccatcgat ggcggctgct gtattggctg
     3121 tcatgttgtc ctccgtagat tcttgttatg aaaaactcag cttacactgt atacctgagc
     3181 tactatacac gtccggacag tgcgaatcca tcgctgcaac agcaaaattc ccgcgctgaa
     3241 ttgccttgat ccgaagcgga tttggcagcc gattttcagg aacagataac gtccggcata
     3301 acggattcaa ccctgcgctg aaccaggcgc ctgtatgcgt ttgcgcagcc ggcgcaggcc
     3361 ccaccagacc acggcgatgc tgcagggcac ggcgatgccg gtgagcagcg ccggatgttc
     3421 cagccagccc tggtctttca ggaattcccc caggtatttc accagcccca ccatgtaata
     3481 actgatggcc gccaccgaca atccttccac cgcctgctgc aattgcagct gcagtttgct
     3541 gcgcttgttc atcgactgca gcagatcctg gttctgcgct tccagtgtaa gattcacacg
     3601 agtgcgcagc aattcgctgg cgcggtcgat ccgctgcgac agatcatcca gttgcccgct
     3661 taccgactga caggtgcgat aggccggcgt caggcggcga cgcagaaatt cactgatagt
     3721 ctgcatgcca cccacttcct gttcgcgcag atccttcagg cggctgatca ccagatcgta
     3781 ataggcgcgc gcggcggaaa agcggtaatt gctgtcggaa atcatctgct cgatgcgcgc
     3841 tgccagcgtc gacagttgct gcagcagacg gcgttcgtcg tcgagcccgc gaatcgcatt
     3901 gatctgctgg ttgatgatcg ccagttgctg cgccatgtcg ctcacctgcg gcgcgatggc
     3961 acgggccagc ggaaacgcgg tgagcaacat catgcgatag gtttccactt ccagcagcgc
     4021 gcgcaccaga cggccggact ggcagggatt cagatcgcga ttgaaaatca ggatgcggcc
     4081 gaagccatcg ttgtgaatgc gataggacgt ccacaatcgc gcctgctcat cgccgaccca
     4141 ggaactgatc agacggtggc cctcgaagtg ccggcgctgc tcgtcggcgg tttgcagttc
     4201 caggggcgcg gtgaggatat cgatgtgctg cgcatgaatg acctgaccgg gaatgccgcc
     4261 cagccagccg gccgggagca gtgtcagcgc gttgcaagtg aaaggagcag ccaagagatc
     4321 acgcacgatg aaggtgtaag tggaaaattc gttgtggcgc tcccagcgca gttcaaaacc
     4381 accgaaatcc tggtagtagc aggacgaacc tgacaccggt ggcaccacgg caaagcgctg
     4441 gcacagctgg ccgatatgcc ggtattccgt atccgcccct tgctcgccgt gcaataccgc
     4501 aaactgcgac acccggctgc cggtttccag caccggaaac ggtcgcgtgt gcaattcatt
     4561 gaacagcttt tcccgctcgg gatgaaaccg gagttgtgca acgtcgtggc gggagggtgc
     4621 agtggccacg cgcagcggtg ccagcgtatc ggattcggtc atggggaaaa cctcgagaag
     4681 cctggaacgg cggagtgtac ccgaacccgg cgcgggagcg gtgactgcga ggtcggcgga
     4741 aatgctggtt gcgctataag gaaggatgcg gacgcttgcc gctgccgccg gcacagcgcg
     4801 gcggcaacgg cagacctgtc agaggtgaag cgctagcgac tgcgcaaagt gacgaacccc
     4861 gctaaacgct cagtcatgaa ataccgtggt agcctccaat tcagcacggc ccagacgggc
     4921 gttgttgatc ggatcggccg tgtcttcgta accgaaggaa ataccgaaca gcagtttgtt
     4981 ggcgggatcg atgcccagca cttcacgcac cagatcggca tggaaaccca gcgatgtttg
     5041 cgggcagcta cccaggccga acgccatcat tcccagcatc agggattggg cgtacatacc
     5101 gacgtccgcc gcttcgcgaa tgccaaatgg ctccggcaaa aacagaaacg ccacgtgggg
     5161 cgcgttgaag aaacggtagt tgcgcagaaa cacttccagc cgcttctgct tttcctcgcg
     5221 ggcgatgccg accgcgttgt acatctgcac cgccgcgtcg tactggcgtt ccttgtagac
     5281 gccgtcgtat ttgccgctgt agggataatc cagggtcatt tttcccagca tcgcattttc
     5341 gtacatgcgc tggcgcaggg tctccagttt ttcaccgctc accacatggg tcagccaggg
     5401 ctgggtgttg caattggacg gtgtgcgctg ggccagggtg aaaatggtgc ggatggtatc
     5461 ggacggcact ggcgtggaca ggaagccgcg cacggagcgg cggcgattga tgatgtccgt
     5521 gaagctggcg gacagggtgg cggtgtcgat tggactggtc atgcaaactt ccctcggaaa
     5581 gcgccgcgat caggtcaggg cgcgaaaatg ataaacaccc tccgtcagcg tgcgctcgac
     5641 ccttcccaga gccagcagcc gatgcaggtg tgccatgcat tctcccaccg ccagaaaatt
     5701 ctggaaagca tccagtttgc ggtcgaacag cttttcggtg agcacctggg cggtggcggg
     5761 ttgctggcag agggtatgaa tctggtccat gcgctgctgg tggtgatgca ccacttgcgc
     5821 cgcccgcaca tgcacgccgg tgaagggcag ctggtgggcc ggcagcacgg tggcttcgtc
     5881 gggcacgatc gccgtcagtt tgtcgtgggc gtcgagccag tctgccagcg gatcggcttc
     5941 cggtgcgacg ggcgatacgc tgacgttcga ggtgattttc ggcagcagct ggtcgccgga
     6001 gatatacagg ttgcgctcgc ggcaatacag cgacagatgt tccggcgcgt gaccccgggt
     6061 ggtgatggcc tgccattcga aatcaccgat gcggacgcgc tggccgtccg tgatgcggtg
     6121 atagctggca ggcatttccg acaccatgtg gttgtaatca tcgtttgcat aaagcgattg
     6181 ctggctgtcg agggacatgc cggcccgcgc gaaatactga tccacttccc agtaaggttg
     6241 gctgcgacgc ggcgcgttga aggcgaggga ttggaaatat tcgctttctg acatgtacag
     6301 cgggatgcgg aagcggtcgc acaaccagcc ggcgaggccg acgtgatcag gatgatggtg
     6361 ggtgacgatg acgcgcgtca gcggcgcgcc ctgcaattcg gtctcgatca cgtccagcca
     6421 ttgctggcgc gcggcggcgc cgttcatgcc ggtgtccagt acgcaccagc cgtcaccgtc
     6481 ccgcagcaga tagcagttga tatgtcccag cgagatgggc aacggtgaac gcagccattt
     6541 cacatccggc gtgatgctga cggcgttgcc ggtgcccggc ggatcggtga acggatagtg
     6601 gaggcccatc cgttacagcc gcttggcaac ttcgccgtac atgacctcgg tggcgccacc
     6661 accgatgggc aatacgcgcg cgtcgcggta catgcgttcg atcacgcatt cctgcatgaa
     6721 gcccatgccg ccgtgcagtt gcacgcagtc gtacatgact tcattggcaa tttcgcaggc
     6781 gttggccttg agcatggaga cttccttgat gcagtcggcg ccctgtgcgt cgagccaggc
     6841 aacatagtgc aggtagttgc gggccagtgc aatcttggtc tgcaggcggg ccagtttgtc
     6901 gcggatgatg tccttctcga acaggggcgc gccgaaggct ttgcgggtct tgacgtgctc
     6961 aagagtgatg tcgagcgcct t
//
LOCUS       DASUEG010000652         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_187009, whole genome shotgun sequence.
ACCESSION   DASUEG010000652 DASUEG010000000
VERSION     DASUEG010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187009"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFX11_12310"
     CDS             <1..389
                     /locus_tag="VFX11_12310"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX4939451.1"
                     /translation="MGYYVKDDSDITWILTTGPGYQMTRFSQTPDNEDRTVDSAALFL
                     STQYEQELSDDVDLEGSYQVIFSDEESGRAKHHAEISLEVDLTDKLDLMVAIYWDRTE
                     KPQPDDEGIAPEPDDAQLAVGLNLEF"
     gene            474..1412
                     /locus_tag="VFX11_12315"
     CDS             474..1412
                     /locus_tag="VFX11_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4939452.1"
                     /translation="MARIEIKHLRTLVALRDCGSLVDAAQQVHLTQPALSHQLKELED
                     RLGQPVVIRKSKPLKFTDSGQRLLQLADRVLPAVDQTEQDILRLAAGKHGRLHIAIEC
                     HSCFEWLMPTIDEYREDWPDVEIDLISGFNFDPLPALSRAELDLVITSDPQPLPGIRY
                     IPLFEYEALLCVGKNHPLAKKDVIEPQDLAAETLITYPVEQGRLDVFKYFLQPAGVTP
                     AAIRTAELTLMIIQLVASARGVCVLPNWGIADYLAKGYVVARSLGDDGVWAKLYAAVR
                     DESAQLPYMEEFIHTAREISQKTLKGILQVELGHSV"
     gene            complement(1437..2114)
                     /locus_tag="VFX11_12320"
     CDS             complement(1437..2114)
                     /locus_tag="VFX11_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4939453.1"
                     /translation="MRGYGQFCPIAKASEVLGERWTHLVIRELSLGSDSFNDLRKGLP
                     LISPSLLSSRLKSLEDAGIVQRQSRGSHVRYLLTKAGDELVPIILQLGTWGHRWVRSD
                     LSSADLDPGLLVWDIHRTLKADFFTAERTVIRIEFTDMASAKRFWWLVIRDGDVDVCL
                     KDPGFDVDLTISSDLQTLTAVWMGDTTIMKAMRENQVQVVGAPNLRKTISGWLGTSYF
                     ADVKAAR"
     gene            2389..>2843
                     /locus_tag="VFX11_12325"
     CDS             2389..>2843
                     /locus_tag="VFX11_12325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4939454.1"
                     /translation="MNAITNTATASQPQTDIEKIKARQKATWEDGDYADFATYMHAGA
                     VDILQSWDLAPAQSLLDVGCGSGQTAIPAAKKGLQVTGIDIAANLIDHARQAAARAKL
                     DVQFDVGDAEDLPYADQHFDVAISLIGAMFAPRPDKVAAELARVIKPGGR"
BASE COUNT          644 a          876 c          780 g          543 t
ORIGIN      
        1 gcatgggcta ttacgtgaag gatgacagcg acatcacatg gatcctgacc accggccccg
       61 gctaccagat gacccgtttc agccaaacac cggataacga agacagaacc gtggattcgg
      121 cggcgctgtt cctctccacc cagtacgaac aggaactgtc ggacgatgtg gatctggaag
      181 gttcctacca ggtgatcttc agcgacgaag aatccggccg cgccaaacac cacgctgaaa
      241 tctcgctgga agtggatctg accgacaagc tggatctgat ggtggccatc tactgggacc
      301 gcacggaaaa gccacagccg gatgacgaag gtattgcgcc ggaaccggac gatgcgcaac
      361 tggcagtcgg tctgaacctg gaattttgaa ttgcagtcca acgctcccgc aatccggctc
      421 cactgaaagc ctgttaaact gctgacattc cttttccatc gccggatcac aacatggccc
      481 gcatcgaaat caaacacctg cgcaccctgg tcgccctgcg cgactgcggc agcctggtgg
      541 acgctgccca gcaggttcac ctcacccagc ctgccctgtc ccatcaactg aaagaactgg
      601 aagaccgcct gggccagccg gtggtgatcc gcaaatccaa gccgctgaaa ttcactgaca
      661 gcggccagcg cctgctgcaa ctggctgacc gcgtgctgcc cgccgtggac cagaccgaac
      721 aggacatcct gcgcctggcc gctggcaagc acggccgcct gcacatcgcc atcgaatgcc
      781 acagctgttt cgaatggctg atgcccacca tcgatgaata ccgcgaagac tggcccgacg
      841 tggagatcga cctgatcagc ggtttcaact tcgatccgct gccggccctg agccgtgccg
      901 aactggatct ggtgatcacc tccgacccgc agccgctgcc cggcatccgt tatattccgc
      961 tgttcgaata cgaagctctg ctgtgtgtgg gaaaaaatca tccgctggca aaaaaggacg
     1021 tgatcgagcc gcaggatctg gcagcggaaa cgctgatcac ctatccggtg gaacagggcc
     1081 ggctcgacgt tttcaaatat ttcctgcaac cggcgggcgt cactcctgcc gcgatccgca
     1141 cggcagaact gaccctgatg atcatccagc tggtggcgtc ggcgcgcggt gtctgcgtgc
     1201 tgcccaactg gggcatcgcg gactatctgg cgaagggcta tgtggtggcg cgttcactgg
     1261 gggacgatgg cgtgtgggcc aaactgtatg ctgccgtgcg cgacgaatcc gcgcagctgc
     1321 cctacatgga ggaattcatt cacaccgcgc gtgaaatcag ccagaaaaca ctgaaaggga
     1381 ttttgcaggt tgagctgggg cattcggtct gaccgtctgc ctgatggcga ggccgactat
     1441 cttgctgctt tcacatccgc aaaataactg gtgcccagcc agccggaaat agtcttgcgc
     1501 agattcggcg cgcctaccac ctgcacctga ttttcccgca tcgccttcat gatggtggta
     1561 tcccccatcc agaccgcagt gagggtttgc aggtcggatg aaatcgtcag atccacatcg
     1621 aatcccgggt ccttcaaaca cacatccaca tcgccatcgc ggatcaccag ccaccagaaa
     1681 cgtttggcgg atgccatatc ggtaaattcg atccggatca ccgtgcgctc cgccgtaaaa
     1741 aaatccgcct tcagggtgcg atgtatatcc cacaccagca aaccggggtc cagatccgcg
     1801 ctggacaagt cactgcgcac ccagcgatgt ccccaggtgc cgagttgcag aatgatcggc
     1861 accagttcat cgccggcctt cgtcaacaga taacgcacat ggctgccgcg ggactggcgc
     1921 tgcacaatgc cggcatcctc cagcgatttg agccgactcg acagcagcga cggcgagatg
     1981 aggggcagtc ccttgcgcag atcattgaat gaatccgaac ccagactcaa ctcacggatc
     2041 accagatgag tccagcgctc gccgagcact tccgacgcct ttgcgatcgg gcagaattgt
     2101 ccatatccgc gcattgccct gctctcccgt tggttccttg attccacttg gacggtattg
     2161 aatgagggta cgcaaaaaca ggaacgcaga ccagtacaga ttctgtacct gctttcgctg
     2221 aatatttgca cagacttggg atcagaccac tgtttttatt cgggattgtc gtgcgcccaa
     2281 tcgttgccct gcgtgcgcag gcgatacaaa cgttgtactg gttgtgaaat cacgccggcg
     2341 ccacactctg ctgcgtgttg aacctgcaca ggaaaaggga gaacgaccat gaacgcaatc
     2401 accaacacag caaccgccag ccagccgcaa accgacatcg aaaaaatcaa ggcccgacag
     2461 aaagcaacct gggaagacgg tgactatgcc gactttgcca cctacatgca tgccggcgcc
     2521 gtggacattc tgcaaagctg ggatctggca ccagcgcaat cgttactgga tgtcggttgc
     2581 ggttccgggc aaaccgcgat tccggccgcc aagaaaggat tgcaggtcac cggcattgat
     2641 atcgccgcca acctgattga ccatgcccgc caggccgccg cccgggcaaa actcgacgtg
     2701 caatttgatg tgggcgatgc agaagatctg ccctatgccg accagcattt tgatgttgcc
     2761 atttcgctga tcggtgccat gtttgcgcca cgccccgaca aggtggccgc tgaactggca
     2821 cgggtcatca aacccggtgg acg
//
LOCUS       DASUEG010000653         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1431965, whole genome shotgun sequence.
ACCESSION   DASUEG010000653 DASUEG010000000
VERSION     DASUEG010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431965"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2625
                     /locus_tag="VFX11_12330"
     CDS             <1..2625
                     /locus_tag="VFX11_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4939455.1"
                     /translation="GSITVQGDKEHPANFGRLCSKGSNLAHTLRSDGRLLHPQIHGIR
                     ASWTQAIDFVAQQFLRTIEQYGPDAVAFYVSGQLLTEDYYVANKLMKGFIGSGNIDTN
                     SRLCMSSAVAGHKRAFGEDSVPGCYEDLEIADLIVLTGSNTAWCHPVLFQRIKAAKQN
                     NPAMKVVVIDPRETATCEIADLHLALKPGSDVTLFNGLLQYLAQQDCLDQSYIAAHTE
                     GFADAIASARADAPTPESVASACDIPAERIRTFYDWFARTGKTVTAFSQGVNQSSSGT
                     DKVNAIINCHLATGRIGKPGASPFSLTGQPNAMGGREVGGLANQLAAHMDFYPEQVDR
                     VRRFWNAPRMASGPGLKAVELFDAVEAGKVKAIWIMGTNPVVSLPNADQAIRALQACP
                     LVVVSDCIAKTDTTALAHVLLPAAGWGEKDGTVTNSERRVSRQRPLLGTLGEARPDWR
                     ILTDVARAMGFSNHFQYENPLDIFREHAALSAFENSDGSWRAFNIGGLQDLDGAGYDA
                     LQPVQWPVLDGTAGSGTLSGALSGTTRLYADGRFSTPSGKARFIALQTRAVANAVSPE
                     YPLALNTGRVRDQWHTMTRTALAPPLNAHKPEPFVEIHPDTAARFHISNGDYVRIRSP
                     WGSMQARATVTSGVRASDVFVPMHWNEQFARNGRVGALVNPATDPYSGQPESKHTPCT
                     IEAWQPDWIGFLFSRLDLPLPDCAWAVKVRGEQFFRYELAGKGSVGDWTTLAHRLGLA
                     LPQGETPSLLYEDPRQGSFRWASFDGDQLQSCAFAGPASSPALLGADRRWLASLFAKP
                     ALDALDRKALLSGKSPAGVEDCGRTVCACFGVGEKTILKAIREHRLTSAADVGKHLKA
                     GTNCGSCVAEIKTLLAGG"
BASE COUNT          651 a         1128 c          930 g          563 t
ORIGIN      
        1 ggcagcatca ccgtgcaggg cgacaaggaa catccggcga atttcggcag gctctgctcg
       61 aaaggttcca acctggccca caccctgcgc agcgacggcc gcctgctgca tccgcagatt
      121 cacggcattc gcgccagctg gacccaggcc attgatttcg tggcgcagca gttcctgcgc
      181 accatcgaac aatacgggcc cgacgcggtg gcgttttatg tgtccggcca gttgctgacg
      241 gaagactatt acgttgccaa caaactgatg aaaggcttca tcggcagcgg caatatcgac
      301 accaattccc gcctctgcat gtcgtccgcc gtggccggcc acaagcgcgc ctttggcgaa
      361 gacagcgtgc ccggctgcta cgaggatctg gaaatagccg acctgatcgt gctcaccgga
      421 tcgaacaccg cctggtgtca ccccgtgctg ttccagcgca tcaaggcggc gaagcagaac
      481 aatcccgcca tgaaggtggt ggtgatcgat ccacgcgaaa ccgccacctg cgaaatcgcc
      541 gacctgcacc tggcgctgaa gcccggcagc gacgtgacgc tgttcaacgg cctgctgcag
      601 tatctggcgc aacaggattg tctggatcag tcttacatcg ccgcgcacac cgagggtttt
      661 gccgacgcga ttgccagtgc ccgcgccgac gcgcccacgc ccgaatccgt cgccagcgcc
      721 tgcgatattc ccgctgaaag aattcgcacc ttttatgact ggttcgcccg caccggcaaa
      781 accgtcaccg ccttttccca gggtgtgaac cagtccagca gcggcaccga caaggtcaac
      841 gccatcatca actgccacct ggccaccggc cgcatcggca agcccggcgc gtcgccgttt
      901 tcgctcaccg gccagcccaa cgccatgggc ggacgcgagg tgggcgggct ggccaaccag
      961 ctggccgcgc acatggattt ttatcccgag caagtggacc gcgtacgccg tttctggaac
     1021 gcgccgcgca tggccagtgg cccgggtctg aaggcggtag agctgttcga cgcggtggaa
     1081 gcaggcaagg tgaaggcgat ctggatcatg ggcaccaatc cggtggtgag cctgcccaac
     1141 gccgaccagg ccatccgcgc gctgcaggcg tgcccgctgg tggtggtgtc ggactgcatc
     1201 gcgaaaaccg acaccacggc gctcgcccac gtattactgc ccgccgccgg ctggggggaa
     1261 aaggatggca ccgtcaccaa ttccgaacgc cgcgtctcgc ggcaacggcc gctgctgggc
     1321 acgctgggtg aagcccggcc tgactggcgc attctgaccg acgtggcacg ggccatgggc
     1381 ttttcgaatc acttccagta cgaaaacccg ttggatattt ttcgcgaaca tgcggcactc
     1441 agcgcgtttg aaaattccga tggcagctgg cgcgcgttca acattggcgg actgcaggac
     1501 ctcgatggcg caggctacga tgcgctgcag ccggtgcagt ggccggtgct ggacggcacg
     1561 gcaggttccg gcacactttc cggcgctctt tccggcacaa cgcgcctgta cgccgacggc
     1621 cgcttcagca cgccctccgg caaagcccgc ttcatcgcgc tgcaaacccg cgcggtggcc
     1681 aacgccgttt cacccgaata tcctctggca ttgaacaccg gccgcgtccg cgaccagtgg
     1741 cacaccatga cccgcaccgc gctggcaccg ccgctcaacg cccacaagcc ggaacccttc
     1801 gtggaaatcc acccggacac cgccgcgcgt ttccacatca gcaacggcga ctacgtgcgc
     1861 atacgcagcc cctggggcag catgcaggcg cgggctaccg tcaccagcgg cgtacgtgcc
     1921 agcgacgtct tcgtccccat gcactggaac gaacagttcg cccgcaacgg ccgcgtcggt
     1981 gcgctggtga acccggccac cgatccctac tccggccagc ccgaatccaa gcacaccccc
     2041 tgcaccattg aagcctggca accggactgg atcggcttcc tgttttcgcg gctggacctg
     2101 ccgctgccgg actgcgcctg ggcggtgaaa gtgcgcggcg aacagttctt ccgctacgaa
     2161 ctggcaggca agggcagcgt gggcgactgg accaccctgg cccaccggct ggggctggcg
     2221 ctgccgcaag gcgaaacacc ttccctttta tatgaagatc cgcgccaggg cagtttccgc
     2281 tgggccagtt tcgacggcga tcaactgcaa agctgcgcct tcgccggccc ggccagctcc
     2341 cccgccctgc tgggcgccga ccgccgctgg ctggcctcgc tgttcgccaa acccgcactg
     2401 gatgccctcg accgcaaggc cctgctgtcc ggcaaaagcc ccgctggcgt ggaagactgc
     2461 ggccgcaccg tgtgtgcctg cttcggcgta ggcgagaaaa ccatcctcaa ggccatcagg
     2521 gaacaccgcc tcaccagcgc cgccgacgtg ggcaagcacc tgaaagccgg taccaactgc
     2581 ggttcctgcg tggcggaaat caaaaccctg ctggcgggcg gctgaaaatc gccaactgcc
     2641 cagccggcac ggtgccattc ccaaaaaaac ctggccccac aaacactccg gccccataca
     2701 ggcaccggtc ccgcgccccg ttccacagca ttcgccatca ccgcatttct tcatccactt
     2761 aacaaaatgg caaaacgacc gccgcagccc ggccggctgt tttgcaccgc aatgcaacac
     2821 gatcgacggt ggcacgctcc attaacgaac ttttattcgc tacgcactgt tttggacgcg
     2881 aaccatagaa gtgcatcaac acaatctcag cgaaccacaa gcgtgcattt ttcaattttc
     2941 acccccgcaa ccccctgatt tctgactttt tttaaactcc atcacgacaa attccgcagt
     3001 gggcacacta gttgctaaat cagacttcga gaagttttta ctatcggcca aaaccgggcc
     3061 tgcgcttacg ccccggtttg tataggtaaa ctggagtctg ctgtgagcaa gcaaaaacta
     3121 gtagtgatcg gaaatggaat ggtcggccag aagttcctgg cccagctggc tgaaagcgac
     3181 caggccgccg cctttgaagt caccaccttc tgcgaagaac cccgtcccgc ttacgaccgc
     3241 gtctatctgt cggcgtattt cagcggcaag ac
//
LOCUS       DASUEG010000654         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_227321, whole genome shotgun sequence.
ACCESSION   DASUEG010000654 DASUEG010000000
VERSION     DASUEG010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227321"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFX11_12335"
     CDS             complement(<1..1033)
                     /locus_tag="VFX11_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4939456.1"
                     /translation="MRTFSILILAVFIAAFAWLQQYTAPLYDGEVYVIEDIYSDITLE
                     KARKLDWSREAPSPVNFGFTASAFWFYLPLPDYILTSPLAHMLEIDFPSIDELDVYLL
                     DSAGAVVEQFHTGTDRPFATRPIWDDDWVFPLTPAQRPAAVLMRAATSNSLQMPIRIY
                     EQVEFYRQDTSNLLFWGGFYGVVLIMALYGFLNGIVISDRLYLYYGAYVVASTLTVAS
                     LNGHGFAYLWPELPEVNAIALSVFPCLLICFGTAFAMEFLDMVRLRFRMRFIGYGFMA
                     VALFTAACAIVLKRDLSFWAAVETVAFSVALLVFGALAVRRQHYLGWYFLIGWSVFLF
                     GAGMVALNVL"
     gene            complement(1039..1992)
                     /locus_tag="VFX11_12340"
     CDS             complement(1039..1992)
                     /locus_tag="VFX11_12340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4939457.1"
                     /translation="MRSTEKNAISSLATSTSFPLLVQQIVGELTQATSYAQVCYLMGR
                     ILGARYQLGLQGLTPEQIEQVRNSLQPLCDESHAWHWAFSKPYGYPGDYMVLEYAYEN
                     VVRPESSPVGQLLDRWLMDSQLAGGVRERKDMLAAFIERKASAHHQITGQPLKVLSLA
                     SGSARELREQTPAVLAKLDVLLIDQDPRSLDYAAQHLPPTVQTWKTNALRLKPADMKH
                     PFDLVYSFGFFDYLKDEVIVHCLNLCRDVLAPGGSIIFPLKMNSKYRHWFYDVFLDWR
                     FVKRELEDGMRLAAQAGLDVVELLETANESVVFYHCVPRQM"
     gene            complement(2269..>3213)
                     /locus_tag="VFX11_12345"
     CDS             complement(2269..>3213)
                     /locus_tag="VFX11_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4939458.1"
                     /translation="LACMFTMMNAARLDVGIEGLAAAELSFQGALAYAKDRLQMRAPT
                     GPVAPEKEADPIIVHPDVRRMLMTQKALAEGCRALAYHIALQLDQEAHGDVDARKAAS
                     DRVALLTPINKAFLTDRGLECANLGVQVFGGHGYIHEHGMEQIVRDVRISLLYEGTNG
                     IQANDLIGRKLISNKGALLRAFLDEIESFCAANSNNPRVGGMVQVLAQHTAEWWQLAQ
                     ELMGKSAAEPNTIASVAVDFQDYAGYVTLAWLWARMALAAAEQLDAGASDAAFYQAKL
                     KTARFYYDRILPRTRSLVATLGCSADSVMDLAVDEFVF"
BASE COUNT          759 a          975 c          883 g          596 t
ORIGIN      
        1 ccagcacatt cagtgcgacc atcccggcgc cgaacagaaa caccgaccag ccgatcagaa
       61 aataccagcc cagataatgt tgccgccgca ctgccagcgc gccaaacacc agcaatgcca
      121 ccgaaaatgc caccgtttcc accgccgccc agaacgacag atcccgcttc agcacaatcg
      181 cacacgccgc cgtgaacagc gccaccgcca taaacccgta gccaatgaag cgcatgcgaa
      241 aacgcagccg caccatgtcc agaaactcca tggcgaacgc cgtgccgaaa cagatcagaa
      301 gacaggggaa caccgacaac gcaatcgcgt tcacctccgg cagctccggc cacagatagg
      361 caaaaccatg accattgagc gacgccaccg tgagcgtgga cgccaccaca taggcgccgt
      421 aatacagata cagccggtca ctgatcacga tgccattcag gaatccgtac agcgccatga
      481 tcagtaccac gccataaaag ccgccccaga acagcaggtt cgacgtgtcc tggcgataga
      541 attccacctg ctcataaatg cggatcggca tctgcagcga attcgatgtg gccgcgcgca
      601 tcagcaccgc cgccggccgt tgtgcaggcg tgagcggaaa aacccagtcg tcgtcccaga
      661 tcggccgggt tgcgaaggga cggtcggtcc cggtatggaa ctgctccacc acagcgcccg
      721 cagaatccag caggtaaaca tccagctcgt cgatggaagg aaaatcgatt tccagcatgt
      781 gcgcgagcgg tgacgtcaga atgtagtccg gcagtggcag gtaaaaccag aaggcggatg
      841 cggtaaagcc aaaattgacg ggcgaaggag cctccctgct ccagtccagt ttccttgcct
      901 tttccagcgt gatatcagaa tagatgtctt caatgacata cacctcacca tcatacaagg
      961 gagccgtgta ctgctgcagc caggcaaagg ccgcaatgaa caccgccagt atcaggatgg
     1021 aaaaggtgcg catggttgct acatctgacg gggaacgcag tggtagaaca cgacgctttc
     1081 gttggcagtt tccagaagct ccaccacgtc cagacccgcc tgcgcagcca ggcgcatgcc
     1141 gtcttccagt tcccgcttca caaagcgcca gtcaaggaac acatcgtaaa accagtgccg
     1201 gtacttgctg ttcatcttca gcgggaaaat gatcgatccg ccaggtgcaa gcacgtcgcg
     1261 acacaggttg aggcaatgaa caatcacttc atccttcagg taatcgaaaa atccgaacga
     1321 atagaccaga tcaaaggggt gtttcatatc ggccggctta aggcgcagcg cgttggtctt
     1381 ccaggtctgc acagtgggag gcaagtgttg cgcagcgtag tccagcgagc gtgggtcctg
     1441 gtctatcagc agcacatcca gcttggccag cacggcgggg gtttgttcgc gtaattcccg
     1501 cgccgatcct gacgccagcg acaacacttt cagtggctgc ccggtaatct gatgatgcgc
     1561 actggccttg cgttctatga aagccgccag catatccttg cgctcgcgca cgccgcccgc
     1621 cagctgcgaa tccatcagcc agcggtccag cagctggccc acggggctgc tttccggccg
     1681 gaccacgttt tcataggcat attccagcac catgtaatcg ccgggataac cgtaaggttt
     1741 gctgaacgcc caatgccacg catgggattc atcgcacaac ggttgcaggc tattgcgcac
     1801 ctgttcaatc tgctccgggg tgagaccttg caggcccagc tggtagcggg cacccagaat
     1861 ccggcccatc agataacaca cctgagcgta actggtggcc tgcgtcagct cgccaacaat
     1921 ctgctgcaca agaagtggaa atgaggtgga tgtggcaagc gatgaaatag cgttcttttc
     1981 agtagagcgc atggtgtttc cccctaaaaa agcacgaatt ttagtcagcg ttgttagcgt
     2041 gcctgactat gtatagaccc atctggtcaa tttcacaaca attacaacaa tatatcaggc
     2101 aatgctaacg gacaaattaa attaggcttt ttttgtggca atttcgagta ggtcaggcgc
     2161 tttgaaggga aataaaaatt aacgggatgt gacagcaaaa aaggcagcgt cgtcggggcc
     2221 gctgcctttt tgcactgcct ttttggcaga aagggaaatg gcgggaattc agaacacgaa
     2281 ttcatccacc gccagatcca tcacgctgtc ggcactgcag ccgagcgtcg ccaccagact
     2341 gcgggtgcgg ggcagaatgc ggtcgtaata gaaacgggcg gttttcagct tggcctggta
     2401 aaaggcggca tcgctggcgc cggcatccag ttgttcagct gcggccaggg ccatgcgcgc
     2461 ccaaagccag gccagagtga cgtaaccggc gtaatcctgg aaatccaccg ccactgacgc
     2521 aatggtgttg ggctcagctg cactcttgcc catcagttcc tgcgccagct gccaccactc
     2581 ggcggtatgc tgcgccagca cctgcaccat tccacccaca cggggattat tgctgttggc
     2641 agcgcagaag gactcgattt catccaggaa ggcacgcagc agcgcgccct tgttcgagat
     2701 cagcttgcgg ccgatcagat cgttggcctg aatgccgttg gtgccttcat acagcaggga
     2761 aatgcgcacg tcgcgcacga tctgctccat gccgtgctcg tgaatatagc cgtggccacc
     2821 gaacacctgc acgccgagat tggcgcactc cagaccgcga tccgtcagaa atgccttgtt
     2881 gatgggcgtg agcaacgcca cccggtcaga cgctgccttg cgcgcatcca catcgccatg
     2941 ggcttcctga tccagttgca gtgcgatgtg gtaggccagc gcacggcagc cttctgccag
     3001 cgccttctgg gtcatcagca tgcggcgcac atcgggatgg acgatgatgg gatcggcctc
     3061 cttttccggc gccaccgggc cagtgggtgc gcgcatctgc aggcgatcct tcgcataggc
     3121 cagcgcgccc tggaacgaca gctccgccgc cgccagtcct tcaatgccca cgtcgagccg
     3181 cgccgcgttc atcatggtga acatgcaggc cag
//
LOCUS       DASUEG010000655         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1810127, whole genome shotgun sequence.
ACCESSION   DASUEG010000655 DASUEG010000000
VERSION     DASUEG010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810127"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFX11_12350"
     CDS             complement(<1..256)
                     /locus_tag="VFX11_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEX4939459.1"
                     /translation="MKPADLLAQPLTLANGTVIKNRFAKSAMSEALGTIDNRVTPHLA
                     TLYGAWARGGAGLVITGNVMIDRRALGEPCNVALEDERDLA"
     gene            complement(360..1382)
                     /locus_tag="VFX11_12355"
     CDS             complement(360..1382)
                     /locus_tag="VFX11_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4939460.1"
                     /translation="MAIFRILSLDGGGVRGLLSATLLDQLEQRVPGWLNNVDMVAGTS
                     TGGIIACGLGHGLTPADICRFYYDRMPKIFADSLLDDITDAGGLIGAQYSLKNLRREL
                     KKVFGDTRLGQLRCRVVIPSFDLDQQAPGKHRHWRPRYFHNFPCEHSHPDEKVVDVAL
                     RTSAAPTFFPTVDGYVDGGVIANNPAMVALSHALDQSLPLPQRPQLDEIRLLSVGTGL
                     VNTFIPGKTHDWGVGQWADPLFRILFDGLIGVPDYQCQQLLGTHYSRLNYSFPAGRDV
                     ALDDWRQRDYLVEVGERRMDAELDQTALWLKNHWLPERPLIPARRRQRARPERQIQQV
                     GIGPMI"
     gene            1480..1761
                     /locus_tag="VFX11_12360"
     CDS             1480..1761
                     /locus_tag="VFX11_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEX4939461.1"
                     /translation="MLKVNEYFDGKVKSIGFKTATLPATVGVMAPGEYTFSTSQKEVM
                     TVVSGALTVLLPGNSNWQTFNAGQDFTIAANQSFQLKVAIDTAYLCTYE"
     gene            1825..2106
                     /locus_tag="VFX11_12365"
     CDS             1825..2106
                     /locus_tag="VFX11_12365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4939462.1"
                     /translation="MARASARHILVSSQEKCEDLKKQILAGADFADLAKQHSTCPSGR
                     KGGDLGQFGPGQMVKEFDQVVFSAPVNTVQGPVKTQFGYHLLEVTSRQD"
     gene            complement(2206..3078)
                     /gene="ylqF"
                     /locus_tag="VFX11_12370"
     CDS             complement(2206..3078)
                     /gene="ylqF"
                     /locus_tag="VFX11_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX4939463.1"
                     /translation="MAINWFPGHMHKARKEIKKIMPEVDLVIEVLDARIPFSSENPLV
                     PALRGNTPALKILNKSDLADPAVTQRWLRHLERTPGVKAVALTQKQPGEVSGLLNLIP
                     ELLKHRNLEKRTVRVLILGIPNVGKSTLINTLAGRAIAKTGNEPAITKMLQRIQLPNN
                     IVLLDTPGFLWPKLSPEECGYRLATTGAIKDTVIEFDDVAMFAIDYLRTAYPDALVNR
                     YQLQEPLPASAVEIMDAIGARRGCLRRGGGADLHKVAEILLNELRSGALGRISLETPE
                     MIAQMMQATRPETE"
     gene            complement(3137..>3430)
                     /locus_tag="VFX11_12375"
     CDS             complement(3137..>3430)
                     /locus_tag="VFX11_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939464.1"
                     /translation="RAMAGKPQVLVLDEATANVDSETEAVVQQSLLALKGSITLVVIA
                     HRLSTITQADNILVLHQGEIMQQGNHRALLEADGLYRHLYELQSTKPELLVQA"
BASE COUNT          661 a         1064 c         1005 g          700 t
ORIGIN      
        1 gggccagatc gcgctcgtct tccagtgcca cgttgcaggg ttcgcccagg gcacgacggt
       61 cgatcatcac gttgccggta atcaccaggc cagcgccacc ccgcgcccag gcgccgtaca
      121 gagtcgccag atggggcgtt acccggttgt cgatcgttcc caaggcttcg ctcatggcgg
      181 atttggcgaa ccggttcttg atgacggtgc cgttggcaag ggtcaggggt tgggcgagca
      241 ggtcggctgg tttcacagga ggcgtctccg ggttcattaa gcatgggaac tgcaatagtt
      301 cgaaagttgt aaaactatcg cagtttgacc caccccgtca aacccgccag cacgcccggt
      361 cagatcattg ggccgatacc cacctgttgg atctgccgtt ccggccttgc ccgctgccgg
      421 cgccgtgcag gaatcagggg ccgttccggc agccagtgat ttttcagcca caaggcagtc
      481 tggtccagtt ccgcatccat ccgccgctca ccgacttcca ccagataatc ccgctggcgc
      541 cagtcgtcca gcgccacatc gcggccggcg ggaaagctgt aattcaggcg ggaatagtgg
      601 gtaccgagca gttgctggca ctggtaatcc ggcaccccga tcaggccgtc gaacaggatg
      661 cggaacagcg gatcggccca ctggcccacg ccccagtcgt gggttttgcc gggaatgaag
      721 gtgttgacca ggccggtgcc caccgacagc aggcggatct cgtccagttg cggtctttgc
      781 ggcaggggca ggctctggtc cagcgcatgg gacagggcca ccatggccgg attgttggca
      841 atcacgccgc catccacata gccgtccacc gtggggaaaa acgtcggcgc ggcgctggtg
      901 cgcagcgcca catccaccac cttttcatcg ggatgactgt gttcgcaggg gaagttgtgg
      961 aaatagcgcg gccgccagtg acgatgcttg ccgggtgcct gctggtccag gtcgaaggac
     1021 ggaatcacca cgcggcagcg cagttgcccc agccgggtgt cgccgaacac ctttttcagt
     1081 tcccgccgca ggtttttcag gctgtattgc gcgccgatca gcccccctgc atctgtgatg
     1141 tcgtccagca gcgagtcggc gaagattttc ggcatgcggt cgtagtagaa acggcagatg
     1201 tccgccggcg tcaggccatg gcccagaccg caggcgatga tgccgccggt ggaggtgccg
     1261 gcgaccatat ccacgttatt cagccagccg ggcacccgtt gctccagctg gtcgagcagg
     1321 gtggcggaca gcaggccgcg cacgccgccg ccgtccagtg aaagtatgcg aaaaatggcc
     1381 atggtgcggg ctccttgccg gtgtgaccgg tctgttggcg cgtactgccc gatcgcgatc
     1441 tcgtggtata ctcgcgctcc ctttttccgg agcattacca tgctgaaagt aaacgaatac
     1501 ttcgatggca aagtgaagtc catcggcttc aagactgcca ccctgcccgc caccgtcggt
     1561 gtgatggcac cgggcgaata caccttttcc acttcccaga aagaagtcat gaccgtggtc
     1621 agcggcgcat tgaccgtcct gctgcccggc aacagcaact ggcagacatt caacgccggc
     1681 caggatttca cgattgccgc caaccagtcg ttccagctga aagtggcgat cgatactgct
     1741 tatctctgta cttacgaata agctacttgc gaataagcta cttgcgaata agctacttgc
     1801 atccaatccg gcaggagaaa caccatggcc agagcctctg cacgacacat cctggtgagc
     1861 agccaggaaa aatgtgaaga cctgaaaaag caaattctcg cgggcgccga cttcgcggac
     1921 ctcgccaaac agcattccac ctgcccatcc ggccgtaaag gtggcgacct cggccagttc
     1981 ggccccggcc agatggtgaa ggaattcgac caggtggtgt tcagcgcacc ggtgaatacc
     2041 gtgcagggac cggtgaaaac ccagttcggc taccacctgc tggaagtgac cagccggcag
     2101 gactgagatt ttgtccgact ggtttcccgg cccactttct ggcgggctcc ctgcaaaaag
     2161 gcggtggcgc atacaccgcc ttttttgttt tcaccgcaaa gcggattact cggtttccgg
     2221 acgggttgcc tgcatcatct gcgcgatcat ctccggcgtt tccaggctga tccgccccag
     2281 tgcaccactg cgcagttcgt tcagcagaat ctccgccacc ttgtgcagat ccgccccgcc
     2341 accccggcgc aggcagccac gccgcgcgcc gatcgcatcc atgatttcca ccgccgacgc
     2401 cggcaacggt tcctgcagct gatagcggtt aaccagcgcg tccggatacg ctgtccgcag
     2461 ataatcgatc gcgaacatcg ccacatcgtc gaattcgatc accgtgtcct tgatcgcgcc
     2521 ggttgttgcc agacgatagc cgcattcttc cggtgacagc ttgggccaga gaaatcccgg
     2581 tgtatccagc aacacgatat tgttgggcag ctgaatccgc tgcagcattt tcgtgatggc
     2641 aggctcgttg ccggtcttgg caatggcgcg cccggccagc gtattgatga gggtcgactt
     2701 gcccacgttg ggaatgccaa gaatcagcac tcgcacggtt cttttttcca gattgcgatg
     2761 cttcagcagc tccggaatca gattgagcag accggacacc tcaccaggct gtttctgggt
     2821 caaggcaacc gccttgacgc cgggcgtccg ttccagatgg cgcaaccagc gctgtgtcac
     2881 cgccggatcg gccagatcgc ttttattcag aatcttgagt gccggggtat tgccgcgcag
     2941 cgcaggcacc agcggatttt cactgctgaa gggaatgcgc gcgtccagca cctcgatcac
     3001 cagatcgact tcaggcatga tttttttgat ttccttgcgg gccttgtgca tgtgcccggg
     3061 aaaccagtta atggccatgg tgcgtacctt ctcggcggct tgaggggatg aatgatagca
     3121 tccagcccct caattttcag gcctgcacca acagctcagg tttagtcgac tgcaattcat
     3181 agagatggcg atacaggcca tcggcttcca gcaacgccct gtggttgccc tgctgcatga
     3241 tctcgccctg gtgcagcacg aggatattgt ctgcctgcgt gatcgtcgac aggcgatggg
     3301 caatcaccac cagcgtgatg ctgcccttca gtgccagcag tgattgctgc accacggctt
     3361 cggtttccga atccacgttg gcggtggctt catccagcac cagcacctgt ggcttgcctg
     3421 ccatcgcccg
//
LOCUS       DASUEG010000656         5418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_555961, whole genome shotgun sequence.
ACCESSION   DASUEG010000656 DASUEG010000000
VERSION     DASUEG010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555961"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1748)
                     /locus_tag="VFX11_12380"
     CDS             complement(3..1748)
                     /locus_tag="VFX11_12380"
                     /inference="COORDINATES: protein motif:HMM:NF005511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4939465.1"
                     /translation="MNQSLATQLSELPGTGVESPQVVNEHSTPWTDKFDVIVVGLGAA
                     GVSAAIEAADRGMRVGAIDRFTGGGTTARSGSVLYAGGGTSLQKKLGVDDSADAMFRY
                     LQTEVQAAVKPETLQKFCTDSPANFDWVTANGVRYSGKLFPSKTSYPVHGNSLYFSGN
                     EIACADLAKPAPRGHLADGIKRFTAFGPAFVGPLLKSARKKGVIPLLQTQVTRLLVND
                     RGDVVGVEAKQIPAKGRAGFLHRLYSTLANYAQIYYAPAAKGLRQKVEQLERQFGRTV
                     RYRAKGGVVLASGGFILNKDMTEKYLPKYRRAMRLGTTGDTGAGIALGVSVGGVAERL
                     GTATAWRFINPPADWPKSALVDTQGQRYINESLYGATIGTKMMEDHNGECILILDDVL
                     FKKSVANLNLDNARFVTWLQAKGALRMAKHADTLEALAQRCKIDPVRLQQTIADYNAA
                     ARGEKPDALGKKTEDMHELKQAPFHAINLSVGKGDITSVITLGGLRVCEKTGAVLKET
                     GAPIRGLFAAGRTAIGVASNAYISGLSIADGVFSGRRAGQYAALCAELPQEHSQPAHT
                     ATPGAAPAAGCPFAH"
     gene            1957..3570
                     /locus_tag="VFX11_12385"
     CDS             1957..3570
                     /locus_tag="VFX11_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939466.1"
                     /translation="MNKLDLSDNKNRILPKLLTLQAEQAGTTEFLITDERRLTFAEAE
                     QLAIAMAAGLRELGVQKDDRVAFYLDNGWEPVLIALAVNKLGAVWVPLNTDYKGAWLS
                     ETIERSRCKVLITETALQDRIIEVQSVLKDVKTVVKGAAEEIRLPGAISFDSLCDHTP
                     IVPDYSSMNYGDTCAILWTSGTTGKSKGVLQSHNSWIRPIAEAASIYFNSQPGDVIYN
                     VLPLYNSGAWITAIFRALYEGIPVVIEKRFSVTTFWDRVRHFGATQTFTLGAMHIFLW
                     NAPAQESDRDHPLRVAQMVPMPEQLREPFAKRFGVELLPMGLGQSEVMVVFTPAHHMG
                     KIPFASLGHPLPDMEIALLDDNNQPVAEGDVGEICLKPLKPFIIFNGYFDDPAATADA
                     FHDGWYHTGDMGKYAAGAYWFVDRKKDALRYAGRNISSMEVEMIVRKHPAVKDVAAFG
                     IPSKEVPGESELALQVVLKPDAQTTSYEELAAFINNNAPYYFVPQYMEFVDTLPYTPT
                     NKVQKFKLREQGVGPNTWVLKKSGYQVQR"
     gene            3571..3969
                     /locus_tag="VFX11_12390"
     CDS             3571..3969
                     /locus_tag="VFX11_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939467.1"
                     /translation="MDDFQLIQLSLEQNAEALGDPTAAVFAQMYERFPALNHFRSDDS
                     SWEQYMMQEILTNLLQFADDPDSAMNTIRDMTSHHRLIGLPLDVFKGMYRTLFELFSP
                     AFAGEQREGMVQAWERLLQRIDSCVDNCPP"
     gene            4112..>5418
                     /locus_tag="VFX11_12395"
     CDS             4112..>5418
                     /locus_tag="VFX11_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4939468.1"
                     /translation="MWYRTFGVLLLWWALHSLASAALPVPVAPDIEQQSLSAGITYRE
                     DVDGGLDLQQVIASRASSAGWQSAGASVPNLGFSRSPFWFHLQLQNTAGVPLQRLLEV
                     EYALLDTVDLYVVQSGAELQHSLAGNRVLFATRVIPHRHLLFPLTLEPHATVDLYLRV
                     KSAYGMQVPLTLWQERAFWVTDQQRLGWQFLYYGLIAVMVLYNLFLAWGVRDAIYLYY
                     VLMVASVGLFQMILHGAAFQFLWPGLPEWNALGIAILTPVANAFSSLFANGILRIREH
                     FPRHYAVQMLQVWLTPVLILASVLMPPDLVMPFSTALVFLTVSATGSAAVQRWRNGGQ
                     EARYFLVAWIVFLLGCLVMALSKFGVLPYNWFTENTMQIGSGIETILLSLALATRINR
                     LQEGQLALEREQLASSQKAIAAEARAIEARYESKAKSDFLAVM"
BASE COUNT          966 a         1587 c         1704 g         1161 t
ORIGIN      
        1 tgtcagtgtg cgaacggaca gccggccgcc ggggcggcac cgggtgttgc cgtgtgcgcg
       61 ggctggctgt gttcctgcgg cagttccgca cacagcgcgg catactgccc ggcgcgacgg
      121 ccggaaaaca cgccgtcggc aatcgacagc ccgctgatgt acgcgttgga cgccacgccg
      181 atcgcggtgc ggccagcggc aaacaggccg cggatcggcg cgccggtttc cttcagcacg
      241 gcgccggttt tctcgcacac acgcaaaccg cccagcgtaa tcaccgaggt gatgtcgccc
      301 ttgcccactg acagattgat cgcatggaag ggtgcctgtt tcagttcgtg catgtcttcg
      361 gttttcttgc ccagtgcatc gggtttttct ccgcgcgcgg cggcgttgta atcggcgatg
      421 gtttgttgca gccgcaccgg atcgatcttg cagcgttgtg ccagtgcttc cagcgtgtcg
      481 gcgtgtttgg ccattcgcag tgcgcccttg gcctgcagcc aggtgacaaa gcgggcgttg
      541 tcgagattca ggttggcgac gctctttttg aacagcacgt catccagaat caggatgcac
      601 tcgccgttgt ggtcttccat catcttggtg ccgatggtgg cgccgtacag ggactcgttg
      661 atatagcgct ggccttgcgt gtccaccagg gcggacttgg gccagtcggc cggcggattg
      721 atgaaacgcc acgcggtggc cgtgcccagc cgttccgcga cgccgcccac gcttacgccc
      781 agggcgatgc cggcgccggt atcgccagtg gtgcccaggc gcatggcgcg gcggtatttc
      841 ggcaggtatt tttccgtcat gtccttgttg aggatgaaac cgccgctggc cagcaccacg
      901 ccgcccttgg cgcggtagcg cacggtgcgg ccgaattgcc gttccagctg ttccactttc
      961 tggcgcagcc ctttggccgc cggcgcgtaa tagatctgcg cgtaattggc cagagtggaa
     1021 tagaggcgat gcagaaaacc ggcgcggcct ttggctggaa tctgtttggc ttccacgccc
     1081 accacgtcgc cgcgatcgtt caccagcagg cgcgtcacct gcgtttgcag cagcggaatc
     1141 acgccttttt tgcgcgcgct tttcagcagc gggccaacaa acgccgggcc gaatgcggtg
     1201 aagcgtttga tgccgtccgc cagatggccg cgcggggccg gtttggccag gtcggcacag
     1261 gcaatttcat tgccggaaaa atacaggctg ttgccgtgca cgggataact ggttttgctg
     1321 gggaacagct tgccggagta gcgcacgccg ttggcggtga cccaatcgaa attggcgggg
     1381 ctgtcggtgc agaatttttg cagggtttcc ggtttcaccg cggcctgcac ttcggtttgc
     1441 aggtagcgga acatggcgtc ggcgctgtcg tccacaccca gttttttctg cagtgatgtg
     1501 ccgccaccgg catacagcac gctgccgctg cgggctgtgg tgccgccgcc ggtgaagcgg
     1561 tcgatggcgc ccacccgcat gccccggtcc gccgcttcga tggcagcgga cacgcccgcc
     1621 gcccccaggc ccaccacgat cacgtcgaat ttgtcagtcc acggggtgct atgctcgttc
     1681 accacctgcg ggctttccac gccggtgccg ggcaattcgc tcaattgggt cgcaaggctt
     1741 tgattcatgg cggatcctgt ttgttcttgt gctggccgga aggatggcgg ttgcgccagt
     1801 ttaacattat gcgataatat tgcaaaaata agtgcataaa tagtccgggc aggccgaatc
     1861 gccttttatc acgtgttccc acacacgttt cagtcgcagt tccaggacca ttagcagttc
     1921 catgaccatt ggcagttcca tcaatgagga agccccgtga acaagctgga tctttccgac
     1981 aacaagaatc gcattctgcc caagctgctg acgttgcagg ctgagcaggc ggggaccacc
     2041 gaattcctga tcaccgacga gcgccggctg accttcgccg aggcagagca gctggccatc
     2101 gccatggcgg ccggcctgcg cgaactgggc gtgcagaagg acgaccgggt tgcgttctac
     2161 ctggacaacg gctgggagcc ggtgctgatc gcgctggcgg tgaacaagct gggcgccgtg
     2221 tgggtgccgc tgaacaccga ttacaagggc gcctggctca gcgaaaccat cgagcgcagc
     2281 cgttgcaaag ttctcatcac cgaaaccgcg ttgcaggatc gcatcatcga agtgcagtcg
     2341 gtgctgaagg atgtgaagac cgtggtgaag ggcgcggcgg aagaaatcag actgcccggc
     2401 gccatcagtt ttgattcgct gtgcgatcac acgcccatcg tgccggatta cagcagcatg
     2461 aattacggcg acacctgcgc gatcctgtgg acgtcgggta ccaccggcaa atccaagggc
     2521 gtgctgcaaa gccacaacag ctggattcgc cccatcgcgg aagcggcttc catctatttc
     2581 aattcccagc cgggcgacgt gatctacaac gtgctgccgc tgtacaactc tggcgcctgg
     2641 attaccgcga ttttccgcgc gctgtacgaa ggcattccgg tcgtgatcga aaaacgtttt
     2701 tcggtgacaa cgttctggga ccgggtgcgt cacttcggtg ccacgcaaac cttcaccctg
     2761 ggcgccatgc acatcttttt gtggaacgcg ccggcacagg aatccgatcg cgaccatccg
     2821 ctgcgtgtgg cgcaaatggt gccgatgccg gaacaattgc gcgaaccgtt cgccaagcgt
     2881 ttcggtgtgg agttgctgcc gatgggcctg ggccagagcg aagtgatggt cgtgttcacg
     2941 cccgcccacc acatgggcaa gattccgttc gccagcctgg gccatccgct gccggacatg
     3001 gaaattgcgc tgctggatga caacaaccag ccggtggcag aaggtgacgt gggcgagatc
     3061 tgcctgaaac cgctgaagcc gttcattatt ttcaatggct atttcgatga tccggcggcc
     3121 accgccgacg cgttccatga cggctggtat cacaccggcg acatgggcaa gtacgccgcc
     3181 ggagcctact ggtttgtcga ccgcaaaaaa gacgcgctgc gttacgccgg ccgcaatatt
     3241 tcctcgatgg aagtggagat gatcgtgcgc aagcatccgg cggtgaagga tgtggcggcg
     3301 ttcggtattc cgtcgaaaga agtgccgggc gaatcggagc tggcgctgca ggtggtgctg
     3361 aagccggacg cgcagactac ctcttacgag gagcttgcgg cgttcatcaa taacaacgca
     3421 ccctattatt tcgtgcccca gtacatggaa tttgtcgaca ccctgccgta cacgcccacc
     3481 aacaaggtgc agaagttcaa gctgcgggag cagggtgttg gccccaatac ctgggtgctg
     3541 aagaaatccg gttatcaggt acaacgctga atggatgatt tccagttgat ccagctgtcg
     3601 ctggaacaga atgcagaggc gctgggtgat cccaccgccg ctgttttcgc gcagatgtat
     3661 gaacgcttcc cggcgctgaa tcactttcgc agtgacgaca gcagctggga gcagtacatg
     3721 atgcaggaaa tcctcaccaa cctgctgcag ttcgccgatg accccgacag cgccatgaac
     3781 accattcgcg acatgacgtc gcaccatcgc ctgatcggcc tgccgctgga cgtcttcaag
     3841 ggcatgtacc ggaccctgtt cgaactgttc agcccggcct tcgcgggcga acagcgcgaa
     3901 ggcatggtgc aggcctggga gcgtctgctg cagcgtattg attcctgcgt cgataattgc
     3961 ccgccctgag ggctcgccag cgattccgcg cggcccttgt tcttgtgctt tacttgtacc
     4021 tgtcttgatg attttataaa ctgcgccggc aatatcaggt cattgatttc attggctttc
     4081 gttgcggggc ggttttccct ggatgaagct gatgtggtat cgcacttttg gtgtgctgct
     4141 gttgtggtgg gcgctgcaca gcctggccag tgctgctttg ccggtgccgg tggcgcccga
     4201 cattgagcag caaagcctgt cggcgggaat cacctatcgc gaggatgtcg acggcgggct
     4261 ggatctgcag caggtgatcg ccagccgtgc ttcgtccgct ggctggcagt cggccggtgc
     4321 cagcgtgccc aacctgggct tttcccgttc ccctttctgg tttcacctgc aattgcagaa
     4381 cacggccggc gtgccgttac agcgtctgct ggaagtcgag tacgccctgc tggatacggt
     4441 ggacctgtac gtggtgcagt cgggagcaga gttgcagcac agcctggcag gcaaccgtgt
     4501 tctatttgcc acccgcgtca ttccccaccg ccatctgctg tttccgctga cactggaacc
     4561 ccatgccacg gtcgatcttt acctgcgggt caagagtgcg tatggcatgc aggtgccgct
     4621 gacgttgtgg caggaacgtg ccttctgggt gacggatcag cagcggttgg gctggcagtt
     4681 cctgtattac ggcctgatcg ccgtgatggt gctctataac ctgtttctgg cctggggtgt
     4741 gcgggacgcg atttacctgt actacgtgct catggtggcc agtgtgggcc tgttccagat
     4801 gatcctgcac ggggccgcct tccagttcct gtggccgggc ctgcccgagt ggaatgcact
     4861 cggaatcgcg attctcacac cggtggccaa tgccttttca tcgctgtttg cgaacgggat
     4921 tctgcgcatc cgggaacact ttccccgcca ttatgccgta cagatgctgc aggtgtggct
     4981 gacaccggtg ctgatactgg ccagtgtcct gatgccaccg gatctggtga tgccgttttc
     5041 caccgcgctg gtatttctga cggtatcggc aacgggcagc gcggccgtgc aacgctggcg
     5101 gaacggtggc caggaagcac gctattttct ggtggcctgg attgtgtttc tgctcggctg
     5161 cctggtgatg gcgctgagca aattcggcgt gctgccttac aactggttca ctgaaaacac
     5221 catgcagatc gggtcgggca tcgagaccat tctgttgtcg ctggcgctgg cgacccggat
     5281 caaccggttg caggaaggcc agctggcgct ggagcgggaa cagctggcgt cgagccagaa
     5341 ggccattgcc gccgaagcca gggcgatcga ggcgcgttat gaaagcaagg ccaagagcga
     5401 ttttctggcg gtgatgag
//
LOCUS       DASUEG010000657         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1771110, whole genome shotgun sequence.
ACCESSION   DASUEG010000657 DASUEG010000000
VERSION     DASUEG010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771110"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1366
                     /locus_tag="VFX11_12400"
     CDS             335..1366
                     /locus_tag="VFX11_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4939469.1"
                     /translation="MQKITSPGRFLTFGAYPRKFVQLLESEWQIPAEPLLAGTGLDRQ
                     QLDDPELLVPFADVVTIFRNGVGRCPAPDLALRYASQLRPSSHGLLGAAVLTGGSLQD
                     AIDLFYDYVGLVAPFLLLHQEDRRNSRVLVFEMISDVQVSATFTYDIMLLSTFNILKL
                     ILGERTRELVFHFAHPAPAHAAIYDTYFDGHVQFNASFYGVSIPRDLLNSRVSTADDD
                     THRLLVSQISSRMEMVHTQNSFVDAVRFHLKRTEGPLPRMSTVAEAFNMSARTFRNRL
                     RRHNTSYQSLLDRERHEQAMNFLRNSEKSVKEIAYILGFQESSNFSRVFKKWTGVTPL
                     DFRRNTVSL"
     gene            complement(1422..1910)
                     /locus_tag="VFX11_12405"
     CDS             complement(1422..1910)
                     /locus_tag="VFX11_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004906495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX4939470.1"
                     /translation="MPSMDVVSELEMFEVNHAVANTVKEIATRFDFRGSKVEVELKEK
                     DKVIKLVAESDFQVDQVRAMLENHLIKRKLDPQCMDVQKMEASGKEIHQTIKLRDGLD
                     SDTAKKIAKAIKESGLKAQASIQGDKVRVTDKKRDVLQAVMAMLREQKLGVPLQFNNF
                     KD"
     gene            complement(1939..>3399)
                     /locus_tag="VFX11_12410"
     CDS             complement(1939..>3399)
                     /locus_tag="VFX11_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4939471.1"
                     /translation="FVKFGQILATRVDLLPPEWIKQLEELQDQTQATPFALLKPQVEA
                     SLGARLTDCFREVDEQPVGTASMAQVHRAVTRSGEEVVLKIRKPGIQDKIEADLRLMR
                     KIAEIAEAQSPEMRRYHPVEIMHEFERSLIRELDFSVEARNADRMRENLASFDFIKVP
                     RIYWQWTCEDLNVQEFIRGIPAKKVDELEQSGADRKQLARRGARAIWKMMLEDGFFHA
                     DPHPGNFIVLPGNRIAMLDFGMVGRLTPTRRDQLIRLIRCVIMQEPENASAVLIEWAG
                     GRFVNREALNAEVSSLLERFDGVPIGELDISALLNDVASILRNHAVRLPSDITLLVKA
                     CVTLEGFGRLIDPDFQLMEEAAPLLKKTLVNRYSPKMLAKSFRVRALDAVERLYNPAT
                     PIFGSAGAGVQVAPSVDPGELEKLVNRLEKASYHQTRAILLAALFIVSGLMLQLEKGP
                     HILGMHVLGLLSLMGCTLYWAWMLINIWWTERGHRS"
BASE COUNT          731 a         1094 c          847 g          727 t
ORIGIN      
        1 ggctcatgtt gttgctgaaa ctcatactgg aactcccgga tcaaaaagcc ccgccatgta
       61 agcggcaacg agggtatcgg tcaagaagaa tggcattcgg ctcccgacat ccccttgccg
      121 ttttggacga ttcgcgcaca acgaaatagt cataaaaagc acagacaata gtcatttcct
      181 gtcattcagg ccgtttatgc atagtttttg tccaacaaaa ccagcctgat gtgccccaaa
      241 tgacgaaccg gactgacggc agggtcaaaa acttcctgac caaaactgtc cataatgcag
      301 ccctcgcaaa gtctggttgg agagaggcaa ggccatgcag aaaatcacct caccggggcg
      361 attcctgacc ttcggcgcct atccgcgcaa gtttgtgcaa ttgctggaat ctgaatggca
      421 gattccggct gagcccctgc tggccggtac cggcctggac cgccaacagc tcgacgatcc
      481 ggagcttctg gttccgttcg cggacgttgt caccatcttc cgtaacggcg tggggcgctg
      541 ccccgcaccg gacttggcac tgcgttatgc atcccagctg cgcccgagct cacacgggct
      601 actgggtgcc gctgtactga ccggaggcag cctgcaggat gccatcgacc tgttttatga
      661 ctacgttggc ttggtcgccc ccttcctgct gctccatcag gaagaccgac gcaacagccg
      721 tgtgctggtg tttgagatga tcagcgacgt gcaggtcagt gccacgttca cctacgacat
      781 catgctgctc agcacattca atatcctgaa gctgatcctg ggcgagcgca cacgcgagct
      841 ggtgttccac tttgcccacc cggcccccgc acatgcggcc atctacgaca cctacttcga
      901 cggccatgtg cagttcaatg ccagcttcta cggcgtttcc atcccgcgtg acctgctgaa
      961 cagccgcgtc tccaccgccg atgacgacac ccatcgcctg ctggtcagtc agatcagctc
     1021 acgcatggag atggtgcata cccagaattc gttcgtggat gcggtccgct ttcacctgaa
     1081 gcggaccgaa ggccccctgc cccgcatgtc caccgtggca gaggcgttca acatgtcggc
     1141 gcgcaccttc cgcaaccggc ttcgccgtca caacacctcc taccagtcgc tgctggatcg
     1201 cgagcgtcat gagcaggcca tgaacttcct gcgcaactcg gaaaagtcgg tcaaggaaat
     1261 cgcctacatt ctgggcttcc aggaaagctc caacttctcc cgcgtattca agaagtggac
     1321 gggagtgacc cctctggact tccggcgcaa caccgtcagc ctctgacttt ctccacccat
     1381 aaaaaagccc ggttgtccgg gcttttttat gggtgcatcc atcagtcctt gaaattattg
     1441 aactgcaacg gcacgcccaa cttttgctca cgcaacatgg ccatgacggc ctgcaacacg
     1501 tcacgcttct tgtccgtcac acgcaccttg tcaccctgaa tgctggcctg cgccttgaga
     1561 ccggactcct tgatggcttt ggcaatcttc ttggccgtat ccgaatccag tccgtcacgc
     1621 agcttgatgg tctggtggat ttccttgccg gatgcctcca tcttctgcac atccatgcac
     1681 tgcgggtcca gcttgcgctt gatcagatga ttttccagca tggcgcgcac ctgatccacc
     1741 tgaaagtcgc tctcggccac caacttgatg accttgtcct tttccttcag ctctacttcc
     1801 accttggatc cacgaaaatc aaaacgcgtg gcgatttcct ttacggtatt cgccaccgca
     1861 tgattgacct cgaacatttc cagttcggat accacatcca tcgacggcat gcttgcctcc
     1921 tctgtttcct gacaacaatt acgaccggtg accgcgctcg gtccaccaga tattgatcag
     1981 catccacgcc caataaaggg tgcaccccat caggctgagc agccccagca cgtgcatccc
     2041 cagaatatgc ggaccctttt ccagttgcag catgagaccg ctgacaatga acagggccgc
     2101 cagcagaatg gcacgggtct ggtgatagct tgccttttcg aggcgattga ccagcttctc
     2161 cagctcaccg ggatccaccg atggcgccac ctgcacacca gcgccagccg aaccgaaaat
     2221 gggcgtcgcg gggttataga gacgctcgac ggcatccagc gcccgcaccc tgaacgactt
     2281 cgccagcatc ttgggcgaat agcggtttac cagagttttc ttcagcaggg gagcggcctc
     2341 ctccatcagc tgaaaatccg gatcgatcag tcgaccaaac ccttccagcg ttacacaggc
     2401 cttcaccagc aacgtgatgt cactaggcaa gcgcacggcg tgattgcgca gaatggaagc
     2461 cacatcattc agcaatgccg aaatatccag ctccccaatc ggaacaccgt caaaacgctc
     2521 aagcaagctc gacacttctg cattaagtgc ttcgcgattc acgaaccgcc cgcccgccca
     2581 ttcaatcagc acggcgctgg cattttcagg ttcctgcatg atcacgcagc ggatcaagcg
     2641 gataagctgg tcccgacgcg tgggcgtcag ccgtccgacc ataccgaaat ccagcatggc
     2701 aatgcgattg cccggcagca caatgaaatt gcccggatgc ggatcagcgt gaaaaaaacc
     2761 gtcctccagc atcatcttcc agatggcacg ggcaccacgt cgggccagct gtttgcgatc
     2821 cgctcccgat tgctccagtt catccacttt ctttgccggg ataccccgga tgaactcctg
     2881 gacattcagg tcttcgcacg tccactgcca atatatgcgc ggcaccttga tgaaatcgaa
     2941 actggcgagg ttctcgcgca tgcggtctgc attgcgtgcc tccacactaa agtccagctc
     3001 ccgtatcaac gagcgctcga actcatgcat gatttctacc ggatgataac gacgcatttc
     3061 aggcgattgc gcctcagcaa tttccgcaat cttgcgcatg aggcggagat ccgcctcaat
     3121 cttgtcctga ataccgggct tgcggatctt gagcaccact tcttcgccgg agcgcgtcac
     3181 cgcacgatgc acctgggcca ttgaggcagt acccaccggc tgctcgtcga cctccctgaa
     3241 gcagtccgtc aaccgggcgc ccagactggc ctccacctga ggcttcagca aggcaaaggg
     3301 cgtggcctgg gtctggtctt gcagctcttc cagttgcttg atccactctg gtggcaacag
     3361 gtcgacacgc gtggccagaa tctggccaaa cttcacgaa
//
LOCUS       DASUEG010000658         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1999110, whole genome shotgun sequence.
ACCESSION   DASUEG010000658 DASUEG010000000
VERSION     DASUEG010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999110"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFX11_12415"
     CDS             <1..373
                     /locus_tag="VFX11_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4939472.1"
                     /translation="EDGYLYVTGRAKEVIVLGGGKKVYPDEVELALTDCPAFTEVCVL
                     GVPSRHGNEEVCAVVVPAPGTDPDDCAAEVGRALAHLAAFKRPTRVVVRTEPIPRTTT
                     RKAKRAALAAWVATPAHAEAA"
     gene            370..1329
                     /locus_tag="VFX11_12420"
     CDS             370..1329
                     /locus_tag="VFX11_12420"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX4939473.1"
                     /translation="MSLDVYVHDFALSLGDHEYSLEESVAAGRTHTGTQALAEAGFRR
                     HRVCRAEQSAYDLARAAVTPIAGAAEGAGAIIYATCLPLNGNIGDEARFRETRDVKHL
                     MDFPVSHLQADFHLDRAAAIGLGQQACTSMLGSMRLARALLRDEPELGRILCVSADRF
                     PPGAEHEQAYNLISDGGAACIVSREARGYRFLGAHHITNGAMATANDEETAGFYFSYS
                     HRMITELCRKAGITPADVQWIVPQNTNRKAWRILSSVLRFDFQRILMPTIGTAGHCIS
                     GDNIINLKTVQAEGRIQPGDKILLPMAGFGLNWSCILLEKVAD"
     gene            1329..2192
                     /locus_tag="VFX11_12425"
     CDS             1329..2192
                     /locus_tag="VFX11_12425"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX4939474.1"
                     /translation="MERLAKIARKLSDASVKRYNSPYEEFQWPDALSLDQWYFSPELI
                     SIYGLEEFAALDEAQQKKLAFWETVNFFGLNIHGEKALIGGLAEHLYYMDWSPELSEY
                     LHHFLDEENKHMTVFGTFCHRYGKKVYPDKKLGFPRKYEKGEADFLFFAKVVIFEELV
                     DHYNLAMGKDERLHPLAQHINQYHHNDESRHLAFGRELSKKLFEQWAPTWKPETVAGV
                     QSYLANYMVSTWREYYNPAVYADAGLDNGYALYQKAWDAPVCRAHRQAVSKSCIDFFL
                     ENRMMTEAPAV"
     gene            2189..2437
                     /locus_tag="VFX11_12430"
     CDS             2189..2437
                     /locus_tag="VFX11_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939475.1"
                     /translation="MSSTVKNRIRDWILAKNPQVDAAALQDDTALLEQKILTSIQIMD
                     LILFLEHLQERPLDIDQVQPENFHSVNTICAAFFAEAV"
     gene            2439..3536
                     /locus_tag="VFX11_12435"
     CDS             2439..3536
                     /locus_tag="VFX11_12435"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939476.1"
                     /translation="MTSERILVTGADGYIGGMVCDRLLAHTDAQLLLWVRSNDAEEFT
                     RKQSQLEHKYAAQRGRIEIHTGNLYGDAPFATVNPQQVDRIVHAAAVTAFNVKEDVAN
                     AVNREGTRKMLAFARQCPQLRRFCYVSTAYAAGLVDGDIQELPLPAAGPFANHYERSK
                     CEAEAILLNEYGDLPWHIYRVATVLAHNDAGDVTQYNVFHNTMRLLFYGLISMLPGTE
                     ETPIYLVTGEFVADAIAELSVKAVPERQFFHVCHRREESLPIGRLVDICFDVFGNDDG
                     FRKRRILRPLFTDFESFELLAEGLSGLSGQVVSQAVESIRPFAPQMFVTKQFHNDQLR
                     GALDRYRAPDPETLVRNTVQYLVNTKWGREV"
BASE COUNT          702 a         1127 c         1170 g          688 t
ORIGIN      
        1 cgaggacggc tacctgtacg tgaccggccg cgccaaggag gtcatcgtcc tcggcggcgg
       61 caagaaggtc taccccgacg aggtcgagct ggcgctgacc gactgcccgg ccttcaccga
      121 ggtgtgcgtg ctgggcgtgc cgtcgcgcca cggcaacgag gaggtgtgcg cggtggtcgt
      181 gcccgcgccc ggcaccgacc ccgacgactg cgcggccgag gtcgggcgcg cgctcgcgca
      241 cctcgccgcg ttcaagcgcc cgacccgggt ggtcgtgcgc accgagccga tcccgcggac
      301 caccacccgc aaggccaagc gcgccgcgct ggccgcgtgg gtcgcgaccc ccgcccacgc
      361 ggaggccgca tgagcctcga cgtctacgtc cacgacttcg cgctgtcgct cggcgaccac
      421 gagtactccc tcgaggaatc ggtcgcggcc ggccgcaccc acaccgggac ccaggcgctc
      481 gccgaggccg gcttccgccg ccaccgggtg tgccgcgccg agcagagcgc gtacgacctg
      541 gcccgggccg cggtcacccc gatcgccggg gccgccgagg gcgcgggcgc gatcatctac
      601 gcgacctgcc tgccgctcaa cggcaacatc ggcgacgagg cccgcttccg cgagacccgc
      661 gacgtcaagc acctgatgga cttcccggtc agccacctgc aggccgactt ccacctcgat
      721 cgcgccgccg cgatcggcct cggccagcag gcgtgcacgt cgatgctggg cagcatgcgg
      781 ctggcccgcg cgctcctgcg cgacgagccc gagctgggcc ggatcctgtg cgtctcggcc
      841 gatcgattcc cgcccggcgc cgagcacgag caggcctaca acctgatctc cgacggcggt
      901 gccgcctgta tcgtcagtcg tgaggcgcgg ggttaccgct tcctgggcgc ccaccacatc
      961 accaacggcg ccatggccac ggccaacgac gaagaaaccg ccggctttta tttcagctac
     1021 agccaccgca tgatcaccga gctgtgccgc aaggccggca tcaccccggc ggatgtgcag
     1081 tggatcgtgc cccagaacac caaccgcaag gcgtggcgca ttctgtccag cgtgctgcgc
     1141 ttcgatttcc aacgcatttt gatgcccacc atcggtactg ccggccactg catatccggc
     1201 gacaacatca tcaatctgaa aaccgtgcag gccgaaggcc ggatccagcc cggcgacaaa
     1261 atcctgctgc cgatggcggg attcggtttg aactggtcct gcattctgct ggaaaaggta
     1321 gctgactgat ggaacgcctc gccaaaatcg cacggaagct gagtgatgct tccgtcaaac
     1381 gctataacag cccttacgag gaattccagt ggccggacgc cctgagtctg gaccagtggt
     1441 atttctcgcc ggaactgatt tccatttatg gcctggagga atttgctgcg ctggacgagg
     1501 cgcagcagaa aaaactggca ttctgggaaa cggtgaattt tttcggcctg aacattcacg
     1561 gcgagaaagc gctgatcggc ggcctggccg agcacctgta ctacatggac tggtcgccgg
     1621 aactgtcgga atacctgcac cattttttgg atgaagaaaa caagcacatg accgtgttcg
     1681 gtaccttctg ccatcgttac ggaaagaaag tgtatccgga caagaaactg ggatttccgc
     1741 gcaagtatga aaaaggcgaa gcggattttc tgtttttcgc caaggtggtg atcttcgagg
     1801 aactggtgga tcactacaat ctggcgatgg gcaaggatga gcgtctgcat ccgctggcgc
     1861 agcatatcaa tcagtatcac cacaacgatg agtcccgcca tctggcgttt ggtcgcgagc
     1921 tgtcgaaaaa gctgttcgag cagtgggcgc ccacctggaa gccggagacg gtggcagggg
     1981 tgcagtcgta ccttgccaat tacatggtgt ccacctggcg ggagtactac aacccggcgg
     2041 tgtacgcaga tgcgggcctg gacaatggct acgcgctgta ccagaaagcc tgggacgcgc
     2101 cggtgtgtcg cgcgcaccgt caggcggtat ctaaaagctg catcgatttt ttcctggaaa
     2161 accgcatgat gaccgaggcg cctgctgtat gagtagcacc gtaaaaaacc ggattcgcga
     2221 ctggattctg gccaaaaacc cgcaggtgga tgctgccgcg ctgcaggatg acaccgcatt
     2281 gctggaacag aaaatcctga cttcgatcca gatcatggat ctgatcctgt ttctggaaca
     2341 tctgcaggag cgtccgctgg atatcgatca ggtgcagccg gagaatttcc attccgtgaa
     2401 taccatctgc gccgcatttt ttgccgaggc ggtgtgagat gacgtcagaa cggattctgg
     2461 taaccggcgc cgacggctat atcggcggca tggtctgcga tcgcctgctg gcccataccg
     2521 atgcgcaatt gctgctgtgg gtgcggtcga acgacgccga ggaattcacg cgcaagcagt
     2581 cgcagctgga gcataaatac gcggcgcagc gtggccgcat tgaaattcac accggcaatc
     2641 tgtacggcga tgcgccgttt gccacggtga atccgcagca ggtggatcgc atcgtgcatg
     2701 ccgccgctgt caccgccttc aatgtgaagg aagacgtggc caatgccgtg aaccgggaag
     2761 gtacccgcaa gatgctggcg tttgcgcgcc agtgtccgca gttgcggcgc ttctgttatg
     2821 tgagcacggc ctatgccgct ggcttggtgg atggcgatat ccaggagctg ccgctgcccg
     2881 ccgccggtcc gtttgccaat cattacgagc gctccaagtg cgaagcggaa gccattctgc
     2941 tgaacgaata cggcgatctg ccctggcaca tctatcgcgt ggctaccgtg ctggcccaca
     3001 acgacgccgg cgacgtcacc cagtacaacg tattccacaa caccatgcgg ttgttgttct
     3061 atggattgat ttccatgctg cccggcaccg aggaaacgcc gatctatctg gtgaccggtg
     3121 aattcgtcgc ggatgcgatt gcagagttga gtgtgaaggc ggtgccggag cgccagtttt
     3181 tccacgtgtg ccatcgccgc gaggaatcgc tgcccatcgg ccggctggtg gatatctgtt
     3241 ttgatgtgtt cggcaatgac gacggttttc gcaagcgtcg catcctgcgg ccgctgttca
     3301 cggatttcga atcgtttgag ctgctggcag aagggttgag cggactgtcg gggcaagtgg
     3361 tgtcccaggc ggtggaaagc attcgcccgt tcgcgccgca gatgtttgtc accaagcagt
     3421 ttcacaatga tcagttgcgt ggggccctcg accgctatcg tgcgcccgat ccggaaacgc
     3481 tggtgcgcaa cacggtgcaa tatctggtga ataccaagtg gggcagggaa gtctgatgct
     3541 gcaacggctg ggtgcgctgg aacaacgcct gacgcacttt gcggatgtgg atgcgcggtt
     3601 gtggtcggcg cacgcagttg cgaccagtgg tggcagtgcg cgtctgctgc gcgcggcgcg
     3661 gcagtggctg gggcgtgcac cggaagg
//
LOCUS       DASUEG010000659         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1497044, whole genome shotgun sequence.
ACCESSION   DASUEG010000659 DASUEG010000000
VERSION     DASUEG010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497044"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFX11_12440"
     CDS             <1..557
                     /locus_tag="VFX11_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HEX4939477.1"
                     /translation="VFVAAYFLTKDMILATKVLIAASALQIAGYWLLKRTAEKMHLAT
                     FAILVVLGGLTIALQDQAFIMWKPTIVNWLFAIVFLGSQFIGRRNLVRTLVEAFLKQM
                     PHHKLELPEEKWLPLNLSFVVFFVLLGGTNLLVVYNFDQDTWVAYKMVGQSILNMLFL
                     LAQFAYLSRYIREEHNENKPEAQG"
     gene            560..859
                     /locus_tag="VFX11_12445"
     CDS             560..859
                     /locus_tag="VFX11_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4939478.1"
                     /translation="MFYAIISEDVPGSLEKRLQTRPAHLERLLALKAEGRLLLAGPHP
                     AIDSEDPGAAGFSGSLVVAEFESLAAAQAWADTDPYLTAGVYQKVTVKPFKKVLP"
     gene            962..1621
                     /locus_tag="VFX11_12450"
     CDS             962..1621
                     /locus_tag="VFX11_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEX4939479.1"
                     /translation="MKARFLILALVSSLTLPLFANAQTLYVYDSPRIDLRSGAGTEYR
                     ILDFLKSGTPMEKLQESGDWIQVRAGEKEGWVKSEYVVAEPVARDQLERALKEISRLK
                     ADNAAINDQLKTTTSELNTFKSNFEKVSGSATDLQKELNRVANVHRNAISTDAAYRQL
                     QEDAELLKVEIEKLKVENARLVDDKRLEGIKWGAGAVLLGMLLTWLISKSAGKKRRSE
                     W"
     gene            complement(1690..2868)
                     /locus_tag="VFX11_12455"
     CDS             complement(1690..2868)
                     /locus_tag="VFX11_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4939480.1"
                     /translation="MAGPLSTLKVLDFSALLPGPFGTMMLADWGADVIRVESPTRPDM
                     VRMLPPQQDGVSACHAYLNRSKKSLALDLKKPGGVDVIKALVKEYDIVVDQFRPGVMD
                     RLGVGYEALRAVNPGVIYCAITGYGQTGPYKDRAGHDLNYLSIAGITGYNGRKSSGPA
                     PINVQVADVAGGSYHSVMAILAAVVHRQQTGEGQFIDVSMTDAAFSMHALTAPPALVG
                     GVEPVVEETQLNGGSFYDCYETSDGRYLSVGGLEPQFFGQFVTALGHPEWNGRGLAMN
                     VDLQRTLKKEIAAVMITRTYAEWCEVFAALDCCVEPVLTFSEACQHPHILARDLIVDV
                     KTESGTQKQLASPVKFSQTRPRYDYAGVALGKHTREVLEGAGMSAEQIDALMKAGTVM
                     "
     gene            3092..>4052
                     /locus_tag="VFX11_12460"
     CDS             3092..>4052
                     /locus_tag="VFX11_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939481.1"
                     /translation="MKTSTVSVHYLNALLARAQKAGIDTTPYIQRLDMSADILGDPAQ
                     RIPSEQYVRLAMQLVQATGDEFLCVGGKHRTKPGTFALMCHAVINSPNLQRAILRCFQ
                     FYNLFIDDMQFRLQKRGEEAVVTLTFDHPEIDWDHSTTDSCLVLLHRFFSWLIGQRLE
                     LTQVSLSGGVPIVKEEYQRLFRTRIQFNQPVNALHFKLRYLDMPIAQTTQNLKQFLRD
                     APENLVVIPDNDDSLTARIRTLIGTDFRHEFPDFESVAKVMNTTPQTLRRHLKQEGTS
                     YQEIKDNMRRDAAIYYLNRSRHSINEIAELMGFSEPSTFHRAFK"
BASE COUNT          880 a         1304 c         1134 g          734 t
ORIGIN      
        1 tagtctttgt ggcagcgtac tttctgacca aggacatgat cctcgccacc aaggttctga
       61 tcgccgccag cgcccttcaa atcgccggct actggctgct caaacgtacg gccgagaaga
      121 tgcacctcgc cacctttgcc attctggtgg tactgggcgg cctgaccatc gccctgcagg
      181 accaggcctt catcatgtgg aagcccacga ttgtcaactg gctgtttgcc attgtcttcc
      241 tgggtagcca gttcatcggg cgccgtaatc tggtccgtac gctggtggaa gcctttctga
      301 aacagatgcc ccatcacaag ctggagctgc cggaagaaaa gtggttaccc ctgaatctca
      361 gcttcgtggt gtttttcgtg ctgctgggcg ggaccaacct gctggtggtg tacaacttcg
      421 accaggacac ctgggtcgcc tacaaaatgg tggggcaatc gattctgaac atgctgttcc
      481 tgctcgccca gtttgcctac ctgtcccgct acatccggga agagcacaat gaaaacaagc
      541 cagaggccca gggttgacca tgttctacgc catcatcagt gaagacgtgc cgggcagcct
      601 ggaaaagcgc ctgcagaccc gccctgccca cctggaacgc ctgctggccc tcaaagccga
      661 gggccgcctg ctgctggctg gccctcaccc ggccatcgac agcgaagacc ccggtgccgc
      721 gggattctcc ggcagcctgg tggtggccga gtttgaaagc ctggcggctg cacaggcctg
      781 ggcagacacc gatccctacc tgaccgccgg cgtctaccag aaggtgaccg tgaaaccctt
      841 caaaaaagtg ctgccctgat ttcagactgg tgaactgttc acgctccggc gacatcaaac
      901 tgacatacac tgccagccca aggcaggatg caaccaccca aaaacgcaac ggggaaccac
      961 agtgaaagca cgcttcttga ttctggcgct ggtcagcagc ctgacgctgc ccctcttcgc
     1021 caacgcccag accctttatg tctacgacag cccccgcatc gacctgcgtt ccggtgccgg
     1081 taccgaatac cgcattctgg actttctgaa gagcggaacc cccatggaaa aactgcagga
     1141 aagcggtgac tggatacagg tgcgcgccgg ggagaaggaa ggctgggtga agtctgagta
     1201 tgttgtcgcc gagccggtgg cacgggatca actggagcga gcgttaaagg agatcagccg
     1261 gctgaaagcg gacaatgcgg ccatcaacga ccagctcaag accacgacga gtgagctgaa
     1321 cactttcaaa tccaactttg agaaagtctc cggcagcgcc accgacctgc aaaaggaatt
     1381 gaaccgcgtc gccaacgtgc accgcaacgc catctccacc gatgccgcct atcgccagtt
     1441 gcaggaagat gcggaactgc tgaaggtgga aatcgagaaa ctgaaagtgg agaacgcccg
     1501 gctggtggat gacaagcggc tggaaggcat caaatggggt gccggtgccg tgctgctggg
     1561 catgctgctg acctggctga tttcgaaaag cgctggcaag aaacgccgca gcgagtggta
     1621 aggttccagc gggaggtgct gccagatgac ggcacctccc gcaattgtag agacgttgca
     1681 ggtgcgaaat tacatcaccg tacccgcttt catcagcgca tcgatctgct cggcgctcat
     1741 tccagcccct tccagcactt cccgcgtatg cttgcccagt gccacgccgg cataatcgta
     1801 gcgcggcctg gtctgcgaga atttcaccgg cgatgccagc tgcttctgtg tgccgctttc
     1861 ggttttcacg tccacgatca gatcacgggc caggatgtgg ggatgctggc aggcttcact
     1921 gaatgtcagt accggttcga cacagcaatc cagcgcggca aacacttcac accattcggc
     1981 ataggtgcgg gtgatcatca cggccgcaat ttccttcttc agcgtcctct gcagatccac
     2041 gttcatggcc agaccgcgac cgttccattc cgggtgcccc agcgcggtaa cgaactggcc
     2101 gaaaaactgc ggctccagcc cgcccactga cagatagcgg ccatccgagg tctcatagca
     2161 atcatagaaa ctgccaccgt tgagctgggt ttcttccacc accggctcca ccccgcccac
     2221 cagggccggc ggtgcggtca gcgcatgcat ggaaaacgcg gcatcggtca tgctcacatc
     2281 gatgaactgg ccttcaccgg tctgctggcg atgcactacc gccgccagga tcgccatgac
     2341 ggagtgatag gaaccgcccg ccacatccgc cacctgcacg ttgatcggcg ccggacccga
     2401 cgacttgcgc ccgttgtagc cggtaatgcc ggcaatcgac aggtagttca ggtcatggcc
     2461 ggcgcggtcc ttgtaaggac cggtctggcc gtaaccggta atcgcgcagt aaatcacgcc
     2521 tggattcacc gctctcagcg cctcgtagcc gacacccagc cggtccatca cgcccgggcg
     2581 gaactggtcc accacgatgt cgtattcctt taccagcgcc ttgatgacgt ccacgccgcc
     2641 aggctttttc aggtccagtg ccagggactt cttggagcgg ttcaggtagg catggcaggc
     2701 cgacacccca tcctgctggg gcggcagcat ccgcaccatg tccggccggg taggtgattc
     2761 gacgcggatc acatccgcgc cccagtccgc cagcatcatg gtgccgaagg ggcccggcag
     2821 caaggcactg aaatccagca ctttcagggt cgacaaaggt ccagccatgg gaaattccaa
     2881 ccagtaaaat atgagaacaa aaaggcgtcc ggacggaagc gccaccagtg ccatagggta
     2941 ggccgtctac cgctggcggg aaaatgaccg tatagttcaa attacctgat catttgcacc
     3001 agaaacaagg cggccggaac ccggcgggaa ttgccaagcc aggatggctg cggcaagatt
     3061 gcccccgctc accggccgac aagcaggatt catgaaaacc agcaccgtat ccgttcatta
     3121 cctgaatgcc ctgctggcgc gggcgcaaaa ggcagggatc gacaccaccc cctacatcca
     3181 gcgactggac atgtcggcgg acatcctcgg cgatcccgcc cagcgcattc ccagcgaaca
     3241 gtatgtgcgg ctggcaatgc agctggtgca ggccaccggc gacgagttcc tctgcgtggg
     3301 cggcaagcac cgcaccaagc ccggcacctt cgccctgatg tgccacgcgg tgatcaacag
     3361 tcccaacctg caacgggcta tcctgcgctg cttccagttc tataacctgt tcattgacga
     3421 catgcagttc cggctgcaga agcgcggcga ggaagcggta gtcaccctca ccttcgacca
     3481 ccctgaaatc gactgggacc actccaccac cgacagctgc ctggtgctgc tgcaccgctt
     3541 tttcagctgg ctgatcgggc agcggctgga gctgacccag gtctccctgt ccggcggcgt
     3601 acccattgtg aaggaagagt accagcgcct gttccgcacc cgcatccagt tcaaccagcc
     3661 ggtgaacgcc ctgcatttca agctgcgcta cctggacatg ccgattgccc agacaacgca
     3721 gaacctgaag cagtttttgc gcgacgcgcc ggaaaacctg gtggtgatcc ccgacaacga
     3781 cgacagcctc accgcccgca tccgcaccct gatcggcacc gacttccgcc atgaatttcc
     3841 ggatttcgaa agcgtggcca aggtgatgaa caccacgccg cagaccctgc gccggcacct
     3901 gaagcaggaa ggcacctcct accaggagat caaggacaac atgcgccgcg acgccgccat
     3961 ctactatctg aaccgctcgc gccacagcat caacgagatc gcggaactga tgggattctc
     4021 cgagcccagt accttccacc gcgccttcaa ga
//
LOCUS       DASUEG010000660         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_578184, whole genome shotgun sequence.
ACCESSION   DASUEG010000660 DASUEG010000000
VERSION     DASUEG010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578184"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFX11_12465"
     CDS             complement(<1..909)
                     /locus_tag="VFX11_12465"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX4939482.1"
                     /translation="MNTMRKMILGTGLAAALVACGGGGGSDGGGGYGTTGELNLAITD
                     APVDGAEHVWITITGVKLKRSGSDEEKIVDVIGNEITVDLLALSNGTTRLLFTESVAA
                     GEYQWARFQLSDAWIVWEGGGQDDLQLPTQDELKTSGNFSVPSNGVAHYTVDWDLRKS
                     IVEMGNSGNYKLKPVLHLRDDDNVGYIQGHIDETFIATECTGENDIPTLYVYNGHAIV
                     PDDMGGAGVEPIASTELPVSNDFYLGMLNPGSYTLAFTCNGGMDDPETDETSLVVEFL
                     DILEVTVIRGENVEFEDADYPFQEYIP"
     gene            complement(981..>2411)
                     /locus_tag="VFX11_12470"
     CDS             complement(981..>2411)
                     /locus_tag="VFX11_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX4939483.1"
                     /translation="VSTQSFGTLSNWLINLMNILTGNLDEPGGVMFTRPAIDLPGIAE
                     LGGATGSFNSRKSRVRKLPEFGGEFPATTLADEILTEGKGQVKALITIAGNPVLSLPN
                     GKRMEKALGKLDFMIAVDFYLNETTQHADIILPPTGPLEHGHYDLGLSMVTVRNTAKY
                     SPALYQPVPDTRHDWEILLELTRRLHSQSPLKWAAGELQYQAMKRLGVEGMLDLLLRI
                     GPYGTPPDTLSDLQKKLANALYKRLDARSTGRTLLDVSPFSRHTRDRNINLNLKALEK
                     HPHGVDLGPLETCFPERLATQGKVINLVPNLFMKDITRLQKLLEKPAKLGPETFLLIG
                     RRDIRSNNSWMHNSQRLVKGKNRCTALIHPDDAQRLAINDGDPVVISSRVGEIEIPAE
                     ITDRMMPGVISVPHGWGHDRGGSQLGVAVLHAGVSLNDVTDERCVDAITGVAAFSGQS
                     VTVSRIKAENNVVRINDKRLAASSAP"
BASE COUNT          425 a          705 c          749 g          532 t
ORIGIN      
        1 ggggatgtat tcctggaagg gatagtcagc gtcttcgaac tcgacgttct cgccccggat
       61 gacggtcacc tcaaggatgt ccaggaactc gacgaccagg ctggtttcgt cggtttccgg
      121 atcgtccatg ccgccgttgc aggtgaacgc cagcgtgtaa ctgccggggt tcagcatgcc
      181 gagatagaaa tcgttgctga caggaagttc agtggaggca atgggttcaa cccctgcgcc
      241 gcccatgtca tctggcacaa tcgcgtggcc gttgtagaca tacagggtgg ggatatcgtt
      301 ctcaccagtg cattcagtgg cgataaaggt ttcgtcgatg tggccctgga tatagcccac
      361 attgtcatcg tcgcgcagat gcaggacagg cttcagcttg tagttaccac tgttccccat
      421 ctccactatg gatttgcgca gatcccaatc tacggtgtag tgggccacgc cgttggaagg
      481 aacgctgaag ttgccgctgg ttttcagttc gtcctgggtg ggcagctgca gatcgtcctg
      541 gccaccgcct tcccatacga tccacgcatc ggacagctga aagcgggccc actggtattc
      601 gccggccgca acgctttcag tgaacagcag gcgggtagtg ccgttcgaca gcgccagcag
      661 atccacggtt atttcgttgc cgatgacgtc cacaattttt tcttcgtcgg agcccgagcg
      721 tttcagcttc acaccggtga tggtgatcca gacatgctcg gcgccgtcca ccggggcgtc
      781 agtgatggca agattcagct cgccggtcgt gccgtagccg ccaccaccgt cacttccgcc
      841 gccaccgccg caggccacca gtgccgctgc cagtccagtc cccaatatca ttttccgcat
      901 agtgttcatc tgtctgctcc agtttactca ggtgctgtat ctaaaaatcg cagttgttca
      961 agtattgcca atgcatccgg tcacggggcc gacgatgccg ccagccgctt gtcattgatg
     1021 cggacgacat tattttccgc tttgatgcgg ctgactgtca cgctttgacc gctgaaagcg
     1081 gccacgcctg tgatggcgtc cacacaacgt tcgtctgtca cgtcattcag gctgacgccg
     1141 gcatgcagca ccgccacccc cagctgggaa ccgccgcggt cgtgacccca gccatggggc
     1201 acggaaatca cgcccggcat catgcgatcg gtaatctcgg cggggatttc gatttcgccc
     1261 acgcgggaac tgatcaccac cggatcgccg tcattaatgg caagacgctg ggcgtcgtcc
     1321 gggtggatca gcgcggtgca gcgatttttg cctttcacca gacgctggct gttgtgcatc
     1381 cacgagttgt tgctgcggat gtcgcggcgg ccgatcagca ggaaagtttc cgggcccagc
     1441 ttggcgggct tttccagcag tttctgcagc cgggtgatgt ccttcatgaa caggttgggt
     1501 accagattga tgaccttgcc ctgggtggcc aggcgctcgg ggaagcaggt ttccagtggc
     1561 cccagatcga cgccgtgggg gtgcttttcc agcgccttga gattcaggtt gatgttgcgg
     1621 tcgcgggtgt ggcggctgaa cgggctgacg tccagcaggg tacgaccggt gctgcgcgca
     1681 tccagccgct tgtagagtgc gttcgccagc ttcttctgca ggtccgacag ggtgtccggc
     1741 ggagtgccat aagggccgat ccgcaatagc aggtcgagca tgccttccac gcccagccgt
     1801 ttcatggcct ggtattgcag ttccccggca gcccatttca gcggcgactg ggaatgcagg
     1861 cggcgggtca gttccagcag gatttcccag tcgtggcggg tgtccggcac gggctggtac
     1921 agggcggggg aatacttcgc cgtattgcgc acggtgacca tgctcaggcc aagatcgtag
     1981 tggccgtgct ccagcggccc ggtgggcggc aggatgatgt cggcgtgctg ggtggtttcg
     2041 ttcagataga aatccaccgc gatcatgaaa tccagtttgc ccagcgcttt ttccatgcgc
     2101 ttgccattgg gcagcgacag cactggattg ccggcaatgg tgatcagcgc tttcacctgg
     2161 cccttgcctt cggtgagaat ctcgtccgcc aacgtggtgg cggggaattc gccgccgaat
     2221 tccggcagct tgcgcacgcg gctcttgcgc gaattgaaac tgccggtggc gccgcccagt
     2281 tcggcaatgc ccggcagatc gatggccggg cgggtgaaca tgacgccacc gggttcatcc
     2341 agattgccgg tgaggatgtt catcaggttg atcagccagt tggacagcgt gccgaagctc
     2401 tgggtggaca c
//
LOCUS       DASUEG010000661         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2036205, whole genome shotgun sequence.
ACCESSION   DASUEG010000661 DASUEG010000000
VERSION     DASUEG010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036205"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="VFX11_12475"
     CDS             <1..2405
                     /locus_tag="VFX11_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939484.1"
                     /translation="FDNVNPLDARFVLNGNDITADIVIDPATKRATATLPAATILDER
                     SQLQINAGAKSKSVRFFVDRSAPEVVVTRVTPAANAPNTSLTIEGYVTDLSAIQKEGG
                     DQPQLRYGVNAGDLTHFFELDADNRFSVNVTTVPMGNEEPASLESAPLRFLVTDETPA
                     AFGLQTSNDTYAPANRQPPFARAHVSALAFANTINPEINAVIDGLDIEAMIKTRNPVV
                     DVSGVLSDIPGVPEVTIPIPVIDDPNLLGLGMGLSVRVDNLDMASLDVDVAPVSANKP
                     TLVTQVDLDDLDLRVNTSISIELPALPDPAIPFSSSLTNANVHGDVTVALKIMGNDTQ
                     APRLAVDSVDIDLDVTGGDIDLVSFDGLPWPLDVALGTLAEIFIGAVEQYAFEKLEDP
                     LLDLIGDAVKDKVIRQVNKELNLLPSDYTVGLRNRQFSFALTEADIATSADGLSVTLS
                     RGDVAAAPMATDSVAEPGWQLNEGGDFASFAPTTPTENLPYEVGLSLDWDFLNKTLYQ
                     AHGTGIDRFGTTFAGSSVPVVGNQLRNLEFRVQVKPVLAPYLDQPIPRATPALASVNA
                     KEISVALEYRDTTKAEDFHQAAEIRANVRMDVDLGVAANKLQPKLDADPDIRIQSFSI
                     NAGETRLNEAVLQKLVDFIMPQAMPLLVDVLGTIRLPCIKGRSLDVLEVEPGSGGYFN
                     VYANVTADAAACPAGPDVAPPPAPTVHVEFSGCDGPTPEFTVDVFGNGKATDEIRVEL
                     KQAGGNFRRFTGSTYFGISNQNDVFRAQACNEYGCGTYGTDSTGRQRCGGGGGNPPTQ
                     PL"
     gene            2473..3216
                     /locus_tag="VFX11_12480"
     CDS             2473..3216
                     /locus_tag="VFX11_12480"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX4939485.1"
                     /translation="MIRKTMHNRTRSNPMEAFKKIHARALERKGGPKGLAAWLPTPKT
                     KAQLTRIPDHRYVAEMARCVFQAGFVWRVIDQKWPDFETVFFKFAPDKLVLLSPEQWE
                     AIGQDRRVVRNMQKIMSVARNAQFVLETAEEHGSFAKLVANWPEQDLIGLLELMKKRG
                     DRLGGVTGMRVLRNLGKDCFLLSGDVITCLKAAGLDIDKASSKRELKLIQATFNQWHD
                     ETGLPYCQLSRICSCSVGENYSAAFDEDA"
     gene            complement(3245..4096)
                     /locus_tag="VFX11_12485"
     CDS             complement(3245..4096)
                     /locus_tag="VFX11_12485"
                     /inference="COORDINATES: protein motif:HMM:NF037805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4939486.1"
                     /translation="MWRKFLISLVMLLSGSAFGHNYQARLDVAEWRLDPSPLMCRLWQ
                     PVPNYGDAVFEIRAGEPLRFYVDSYRPVIKPGKADMAIVAPEWKPDIQTRPVGRTAVV
                     PGKRPIVLDEDKANLFLAELEVGMFPSFTHHAWFEKHNVTVDVSAVNFQSAYTGYLSC
                     VAGLFPANFDQLQQSLLHFETDKWVIRGRLKERLDLISGYVTLDPAITKIRIDGHTDS
                     DGRRGYNWELSRKRAQAVKDYLESKGVAPEMIEMKYYGEGVPAKNNVNAANKAVNRRV
                     LVRMERG"
     gene            4259..>4772
                     /gene="rnt"
                     /locus_tag="VFX11_12490"
     CDS             4259..>4772
                     /gene="rnt"
                     /locus_tag="VFX11_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX4939487.1"
                     /translation="MSSNKSSLAHRFRGYLPVVIDVETGGFNHQKDALLEIAAVILQM
                     DEHGMLLPGQQIHHHVVPFPGANIDQAALDFTGIDPYHPFRGAVDEVDAIKDIFSAVR
                     LALKDEGCNRAILVGHNAFFDRDFVNAAVERNGIKRNPFHPFSCFDTASIGGVAVGQT
                     VLAKVCKAAHI"
BASE COUNT          984 a         1527 c         1420 g          841 t
ORIGIN      
        1 ccttcgacaa cgtcaatccg ctggatgccc gcttcgtgct gaacggaaac gacatcaccg
       61 ccgacatcgt catcgaccct gccaccaaac gggccactgc cacactgccc gccgccacca
      121 tcctggacga acgcagccag ctgcagatta acgccggcgc gaagtcaaaa tcggtgcgct
      181 tctttgtgga ccgctcggca ccggaagtgg tggtcacccg cgtcaccccg gctgccaatg
      241 cccccaatac cagcctgacc atcgaaggct atgtgaccga tctttccgcg atccagaagg
      301 aaggcggcga ccagccccag ctgcgctacg gcgtcaacgc aggggacctg acccattttt
      361 ttgaactgga cgcggacaac cgcttttcgg tcaacgtcac caccgtgccg atggggaacg
      421 aggaacccgc cagcctggaa tccgcaccgc tgcgcttcct ggtcaccgac gagacgccgg
      481 cggccttcgg cctgcaaacg tccaacgaca cctacgcgcc agccaaccgc cagccaccgt
      541 ttgcccgtgc ccatgtcagc gcactggcgt ttgccaacac catcaatccg gaaatcaacg
      601 ccgtcatcga cgggctggac atcgaagcca tgatcaagac ccgcaatccg gtggtggacg
      661 tcagcggcgt gctgtcggac attcccggcg ttccggaagt gaccattccc attcctgtca
      721 tcgacgatcc caatctgctg ggcctgggca tgggcctgag cgtgcgggtg gacaacctgg
      781 acatggccag cctggatgtg gacgtggcgc cagtgtccgc caacaagccc acgctggtga
      841 cgcaggtgga tctggacgat ctggacctgc gcgtgaacac ctcgatcagc atcgaactgc
      901 ccgcgctgcc ggacccggcg attccgtttt ccagcagcct caccaacgcc aacgtgcacg
      961 gtgatgtcac cgtcgcgctc aagatcatgg gcaacgacac gcaggcaccc agactggcgg
     1021 tggattcggt cgacatcgat ctggatgtga ccggtggcga tatcgatctg gtgtccttcg
     1081 acggcctgcc ctggccgctg gatgtggcgc tgggcacgct ggcggaaatc tttatcggcg
     1141 cggtggaaca gtacgcattt gaaaaactgg aagacccgct gctggatctg atcggcgacg
     1201 cggtgaaaga caaggtgatc cgccaggtga acaaggagct gaacctgctg ccgtcggatt
     1261 acaccgtggg cctgcgcaac cggcaattca gtttcgcgct gaccgaagcc gacattgcca
     1321 ccagtgccga tggcctcagc gtcaccctca gcaggggcga tgttgcggct gcgccgatgg
     1381 ccaccgacag cgtcgccgaa ccgggctggc agctgaatga aggcggtgat tttgcgtcct
     1441 tcgcccccac gacgccgaca gaaaacctgc cctatgaagt gggcctgagt ctggactggg
     1501 atttcctcaa caagacgctg tatcaggcgc acggcaccgg catcgaccgt ttcggcacca
     1561 ccttcgccgg cagctcggta cccgtggtgg gcaaccagct gcgcaacctg gaattccgcg
     1621 tgcaggtaaa accggtgctg gcgccctatc ttgatcaacc gatcccgcgc gccacaccgg
     1681 cactggccag cgtgaatgcg aaggaaatca gtgtggcact ggaataccgc gataccacca
     1741 aagccgagga tttccaccag gctgccgaga tccgcgccaa cgtgcgcatg gatgtggacc
     1801 tgggtgtggc cgccaacaaa ctgcagccga agctggacgc cgatcctgac atccgcatcc
     1861 agtccttcag catcaacgcc ggcgaaaccc ggctgaacga agcggtgctg cagaaactgg
     1921 tggatttcat catgccccag gccatgccgc tgctggtgga cgtgctcggc accatcaggt
     1981 tgccctgcat caagggccgc agtctggacg tgctggaagt ggagccgggc agcggaggct
     2041 acttcaatgt gtacgccaat gtcaccgccg acgcagcggc ctgccccgcc ggcccggatg
     2101 tggcaccgcc gccggcaccg accgtgcatg tggaattcag cggctgcgac gggcctacgc
     2161 cggaattcac cgtggatgtg ttcggcaacg gcaaggccac cgacgaaatc cgggtggaac
     2221 tgaaacaggc gggcggcaat ttccgccgct tcaccggcag cacctatttt ggtatctcca
     2281 accagaatga cgtgttccgc gcccaggcct gcaacgagta cggttgcggc acctatggca
     2341 ccgacagtac cggacgtcag cgttgcggtg gcggtggtgg caatccgcca acgcagccgc
     2401 tgtgaaaaaa cgggcacccg ggctgaccgg gtgcccgcaa atttcgggtg acgcgatttg
     2461 ccggtgtctg ccatgatccg caagaccatg cacaaccgga cgagatcgaa cccgatggaa
     2521 gcattcaaga aaattcacgc ccgcgccctg gaacgcaaag gcggtcccaa aggattggca
     2581 gcctggctgc ccacgcccaa aacgaaggcc cagctgaccc gcatcccgga tcaccgttat
     2641 gtcgccgaaa tggcccgctg cgttttccag gccggctttg tctggcgcgt catcgatcag
     2701 aaatggccgg atttcgaaac cgtattcttc aaatttgcac cggacaagct ggtgctgctg
     2761 tcgcccgagc aatgggaagc catcggccag gatcgccgtg tggtgcgcaa catgcagaag
     2821 atcatgagcg tggcccgcaa tgcgcagttt gtgctggaga cagcagaaga acacggcagc
     2881 ttcgcaaagc tggtggcgaa ctggccggag caggatctga tcggcctgct ggaactgatg
     2941 aagaaacgcg gcgatcgcct gggtggcgtt accggcatgc gcgtgctgcg caatctgggc
     3001 aaggattgtt tcctgctgag tggcgacgtc atcacctgcc tgaaagccgc cgggctggat
     3061 atcgacaagg ccagttcaaa acgcgaactc aaactgattc aggcgacctt caaccagtgg
     3121 cacgacgaga ccggcctgcc ctactgccag ctgagccgga tctgttcatg ctcggtgggg
     3181 gaaaattaca gtgcggcgtt cgatgaagat gcgtgaaaga cggaacgggc cggcagcgct
     3241 gcgcttagcc ccgttccatg cgcaccagca cgcgccggtt aaccgccttg ttggcggcgt
     3301 tgacgttgtt cttggcggga acaccctcgc cgtaatattt catttcgatc atttccggcg
     3361 ccacgccctt ggattccaga taatccttca ccgcctgcgc gcgcttgcgt gacagctccc
     3421 agttatagcc acgccggccg tcgctgtcgg tgtggccatc gatgcggatc ttggtgatgg
     3481 cggggtccag ggtgacataa ccggaaatca gatcgagccg ttccttcagc cggccgcgaa
     3541 tgacccactt gtcggtttcg aaatgcagca gggattgttg cagctgatca aagttggcag
     3601 ggaacaggcc agcgacacag gacaggtagc cggtataggc gctctggaaa ttcaccgccg
     3661 acacatccac ggtgacattg tgcttttcaa accaggcgtg atgggtgaac gacgggaaca
     3721 tgcccacctc cagctccgcc aggaacaggt tggccttgtc ctcgtccagc acgatgggac
     3781 gcttgccggg cacgaccgct gtgcggccga caggacgggt ctggatatcc ggcttccatt
     3841 cgggcgcgac gatcgccatg tcggccttgc caggcttgat caccggacgg taggaatcca
     3901 cgtagaagcg gagcggttcg cccgcgcgaa tttcgaacac cgcgtcaccg tagttgggaa
     3961 ccggctgcca caggcggcac atcagtggtg aggggtccag gcgccattcc gccacatcca
     4021 gccgcgcctg atagttatgg ccgaaggcac tgcccgacag cagcatcaca agagatatca
     4081 aaaacttgcg ccacatgcac ccaatgcccc actcccggtt tgcacaaccc tacccttact
     4141 gtatcggaag cgaatgattt tgcttgagat gccgggcaaa aaatttgtac cccccggcgc
     4201 ggtacgatag catcagcgcc taatcatcgg ggtccggacc cctaagcgag ccccctacat
     4261 gagcagcaac aaatccagcc tggcccaccg ctttcgcggc tacctgccgg tggtgatcga
     4321 tgtcgagacc ggcggcttca accaccagaa ggacgccctg ctggaaatcg cggccgtgat
     4381 cctgcaaatg gacgagcatg gcatgctgct gccgggccag cagatccacc accacgtagt
     4441 tccctttccg ggcgccaaca tcgaccaggc cgccctggat ttcaccggca tcgaccccta
     4501 ccaccctttc cgtggcgcgg tggacgaagt ggatgccatc aaggacatct tcagcgccgt
     4561 gcgtctggcc ctgaaggacg aaggctgcaa ccgcgccatc ctggtgggcc acaatgcctt
     4621 cttcgaccgg gatttcgtca acgcggcggt ggagcgcaac ggcatcaagc gcaacccgtt
     4681 ccacccgttc tcctgctttg acaccgcctc catcggcggc gtggcggtgg ggcaaaccgt
     4741 gctggcaaaa gtctgcaagg ccgcccacat tc
//
LOCUS       DASUEG010000662         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1352027, whole genome shotgun sequence.
ACCESSION   DASUEG010000662 DASUEG010000000
VERSION     DASUEG010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352027"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFX11_12495"
     CDS             complement(<1..742)
                     /locus_tag="VFX11_12495"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEX4939488.1"
                     /translation="MRLHTVSAINSLPPALLDALSVPTANPFFNATFLGLLEQCGCVS
                     AQTGWQPRHIWIERAGEPVFFMPVYQKGHSWGEFVFDQRWAQAYRQQGLHYYPKWITA
                     VPFTPSVGPRWWVKPGENVAALLQLAVEHVQAEVRAMAGSGWHLLFADALPAGFDRRD
                     LLPRHDTQFHWRNYGYASFDDFLARLKSRKRKSIRRERQRVAEQGVTLLTREGSELSD
                     RDWQQFYHCYENTYRVRGQQPYLNAEFFR"
     gene            980..1486
                     /locus_tag="VFX11_12500"
     CDS             980..1486
                     /locus_tag="VFX11_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014110179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4939489.1"
                     /translation="MTSFTLNGKPVQLDAEPEMPLLWAIRDLLGLTGTKYGCGQGLCG
                     ACTVHVDGQAQRSCVTPLSAIAGKAITTIEGLSAAGDHPVQQAWIQNNVPQCGYCQPG
                     QIMSASALLAKNPNPTDSDIDSAMSGNLCRCGTYGRIRIAIQDAASRLNNNNSAAPYA
                     AFQENLGG"
     gene            1535..>2919
                     /locus_tag="VFX11_12505"
     CDS             1535..>2919
                     /locus_tag="VFX11_12505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4939490.1"
                     /translation="MQTRREFLKTLGIAGSGLILPVTLSGCVTAPSTPSQPATWKPNA
                     WLRITPENDILFYLDRVEMGQGTMTGLTTLIGEELNTAPHLIQVITAPVHKDFINPLY
                     GIQITGGSTSMRSDHDRLREAGAEARERLRSAAALRFEVGIDEMQPVDGYIEYNGARI
                     PFGQLAESAGQLSKPKFKLKSPSQYRFIGKPGIRLDAAMKARGTGKFGIDTQLPELYT
                     AVLKRCPVIGGTVKSWQANGAEQMPRVKKVVAVPMGVAVLADSFWHAQKALEKVTVEW
                     ELPELAQRSSADIKTEFARLAKEESGRSTRSDGDAEDAIAEAAHPLKATYTVPYLAHA
                     TMEPMNSTVRLTDDACEIWAPTQAPDVCAAVAEEVSGLRRSQIKVHTTLIGGGFGRRL
                     NVDYAVEAVHIAKASGLPVKLVFSREDDIRHDFYRPAAHTELAAGLDARGRITGWSHK
                     NVCPAILTWSF"
BASE COUNT          612 a          947 c          854 g          506 t
ORIGIN      
        1 gacgaaaaaa ttccgcgttc agatacggtt gctggccccg cacccggtag gtgttttcat
       61 aacagtgata gaactgctgc cagtcgcggt ccgataattc actgccttcc cgcgtcagca
      121 gcgtcacacc ctgttccgcc acccgttgcc gctcgcggcg gatgctcttg cgtttgcggg
      181 atttcagccg ggccaggaaa tcgtcgaagc tggcgtagcc gtaattgcgc cagtgaaact
      241 gggtgtcgtg ccgtggcagc agatcacggc gatcaaatcc cgcgggcaat gcgtctgcga
      301 acagcaaatg ccagccggag cccgccatcg cccgcacctc cgcctgcaca tgttccaccg
      361 ccagctgcag caacgccgcc acattttcgc ccggcttcac ccaccagcgc ggcccaacac
      421 tgggcgtgaa cggcaccgcc gtgatccatt tcgggtagta gtgcaacccc tgctgccggt
      481 aggcctgggc ccagcgctgg tcgaacacaa actcgcccca ggaatgcccc ttctgataca
      541 ccggcatgaa aaacaccggc tcgccggccc gttcgatcca gatatggcgc ggctgccagc
      601 cagtctgcgc gctgacgcag ccacactgtt ccagcaatcc cagaaaagtg gcgttgaaaa
      661 agggattggc cgtgggaaca gataatgcat ccagcagggc gggaggcagg ctgttgatgg
      721 ctgatacggt gtgtaaacgc atacctttct acttctgctt gataagctgg attggccggt
      781 ggttatttga ggatgggtcc ggtagtagcg gcgcggagcc cgaacggaac cataccaccg
      841 gatagctccg catacgtcaa gatccctgcg cacaggttgc aatgggtcaa cattggcgtc
      901 agtgcattcc tctttggacg ggtttacaga tcgccaagcg ctgggtgaga atgcggcagg
      961 acacagagga ctgccagtca tgacttcatt cacacttaac ggaaagccgg tgcaactgga
     1021 cgcagaacca gagatgccat tgctgtgggc catccgcgat ctgctggggc tcacaggaac
     1081 aaaatacggt tgcgggcagg gcctgtgcgg cgcctgcacc gtgcatgtcg atggtcaggc
     1141 gcaacgctcg tgcgtcactc cgctgagcgc catcgccggc aaggccatta ccaccatcga
     1201 aggcctcagc gccgccggtg accacccggt acagcaggcc tggatccaga acaacgtgcc
     1261 ccagtgcggc tactgccaac cggggcagat catgtccgcc agtgccctgc tggcgaaaaa
     1321 tcccaacccg accgattccg atatcgacag cgccatgtcc ggcaatctgt gccgctgtgg
     1381 cacctatggc cgcatccgca tcgccatcca ggatgccgca tctcgactca acaacaataa
     1441 ttccgcagcg ccgtacgcag cgtttcagga aaatctgggc ggctgaaccg ccccgttatt
     1501 tttttggtag cttatttttt cagagggatc cgccatgcaa acccgtcggg agtttcttaa
     1561 aaccctgggg attgccggca gcgggctgat tctgccggta acgctgtccg gctgtgtcac
     1621 cgcgccgtca acaccatcgc aacccgccac ctggaagccc aacgcctggc tgcgtatcac
     1681 gccggaaaac gacatcctgt tttacctcga ccgcgtggaa atgggccagg gcaccatgac
     1741 cggcctcacc accctgatcg gcgaggaact caacacagcg ccccacctga tccaggtgat
     1801 cacggcacct gtgcacaagg atttcatcaa tccgctgtat ggcatccaga tcaccggcgg
     1861 cagcaccagc atgcgttccg accacgaccg gttgcgtgaa gcaggtgccg aagcccgcga
     1921 gcggttgcgg tccgccgccg cactgcgctt tgaggtgggc atcgatgaaa tgcagccggt
     1981 ggacggctat atcgaataca acggcgcgcg gattccattc ggccagctgg cagagtctgc
     2041 cggccagctg tcgaaaccga agttcaaact gaaatcgccg tcgcaatacc gcttcatcgg
     2101 caagcccggc atccgcctgg acgccgccat gaaagcccgc ggcaccggca agttcggcat
     2161 cgacacccag ttgccggaac tgtacaccgc cgtgctgaaa cgctgccccg tcatcggcgg
     2221 caccgtgaaa agctggcagg ccaatggcgc cgagcagatg ccccgggtga aaaaagtggt
     2281 ggccgtgccc atgggcgtcg cggtgctggc agattcgttc tggcacgcgc agaaagcgct
     2341 ggaaaaagtc acggtggaat gggaactgcc agaactggcg cagcgttcca gcgccgacat
     2401 caagacggaa ttcgcccgtc tggccaaaga ggaatccggc cgcagcaccc gcagtgatgg
     2461 cgacgcagaa gacgccatcg ccgaagcggc gcatccgctc aaggccacct acaccgtgcc
     2521 ctatctggcc cacgccacca tggagccgat gaatagcacg gttcgcctga ccgatgatgc
     2581 gtgcgaaatc tgggccccca cccaggcgcc cgatgtctgc gccgcggtgg cagaagaggt
     2641 cagtggcctg cgccgcagcc agatcaaggt acacaccacc ctgatcggcg gcggctttgg
     2701 ccggcgtctc aacgtggact acgcggtgga agcggtgcac attgcgaaag ccagcggcct
     2761 gccagtgaaa ctggtgttca gccgcgagga cgatatccgc cacgatttct accgccccgc
     2821 cgcccacacc gaactggccg ccggtctgga tgccaggggc cgcatcaccg gctggtccca
     2881 caaaaacgtc tgcccggcga ttctcacctg gtccttcca
//
LOCUS       DASUEG010000663         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_342867, whole genome shotgun sequence.
ACCESSION   DASUEG010000663 DASUEG010000000
VERSION     DASUEG010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342867"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFX11_12510"
     CDS             complement(<1..340)
                     /locus_tag="VFX11_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4939491.1"
                     /translation="MSPEFNPFEIASRHASRQVPVVPPSTSVAELKALLGRESFECAS
                     HIVVCEDDRFVGVLRIEDMLPAADNRLVRELMDPDAPTIKPGVDQEKAAWHAVKHNEA
                     ALAVVDENGSF"
     gene            complement(353..547)
                     /locus_tag="VFX11_12515"
     CDS             complement(353..547)
                     /locus_tag="VFX11_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939492.1"
                     /translation="MTDKLQLDPNDFQDPEKMKKIVRVLIMAVAKVPEEFGRDAIWGG
                     LEALEEELAKPVDHRHFTQH"
     gene            complement(649..909)
                     /locus_tag="VFX11_12520"
     CDS             complement(649..909)
                     /locus_tag="VFX11_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX4939493.1"
                     /translation="MSQWTVYMVECSDRTYYTGITTDLSRRLAEHNGTRKGARYTRTR
                     QPVTLVYSELQHDRSAAAAREYQLRKLPRAAKQALIHAAASG"
     gene            1121..>2212
                     /locus_tag="VFX11_12525"
     CDS             1121..>2212
                     /locus_tag="VFX11_12525"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4939494.1"
                     /translation="MLGSQRGLPRLIPPLLCLLFCVLAGFAPASLSQAPVLTVSEEVG
                     HYPLATHLAYVHDSRGEWTIADLVRPDSGILFQPNQEETLAFGYVDAVYWLRLDVNST
                     DRIRTAWVLEIAGLGRAIDEIDLYVPDDDGHWLRQRSGDSVPFSQRSVSHHAVLFAFQ
                     LPSRQSTTLYLRVKTNGTLQIPATLWSSARYLENEHHDSLLEGIFYGVLLIMIFYNGF
                     IYLSIRDNSYIVYVCYLVCVFGFAVSIKGVGFEYLWPEQIWWNNKSNLVFAELGVLFV
                     LLFARSFLNTPKNTRLINYVIAFLILTSIACFPVVVVADHYIAASVLSVQYLLAVVSV
                     VIAASLCLQRGFKAARYYLLAWLGVLVSIL"
BASE COUNT          396 a          620 c          647 g          549 t
ORIGIN      
        1 cgaagctccc gttctcatcc accaccgcca gtgccgcctc gttgtgtttc actgcatgcc
       61 aggcggcttt ttcctggtcc acgcccggtt tgatggtggg cgcgtccggg tccatcagct
      121 cgcgcaccag ccggttatcg gctgccggca acatgtcttc aatccgcagc acacccacaa
      181 accggtcatc ctcgcacacc acgatatggc tggcgcactc gaagctttcc cggcccagca
      241 atgccttcag ttctgcaacg ctcgtggatg gaggaacgac gggcacctgg cgggacgcgt
      301 ggcgcgaggc aatttcaaac ggattgaatt ccggggacat ggaacttgaa aatcagtgtt
      361 gcgtgaaatg gcggtggtcg accggcttgg ccagttcttc ctcgagcgcc tccagtccgc
      421 cccagatggc gtcacggcca aattcctccg gcaccttcgc cacggccatg atcaacacac
      481 gcacgatttt tttcatcttt tccgggtcct ggaaatcgtt ggggtccaac tgcagcttgt
      541 ctgtcatgtt ttctacacct ttgaaaaggc cggttgaacc agccgtacaa ctgccctgtg
      601 caaggggcgg cattctcggc cagttcggcc gtcaaggcaa atgacctgtc agccagatgc
      661 ggcagcatgg atcagggcct gcttggccgc ccgtggcaat ttccggagct ggtattcccg
      721 ggcggcggct gcggatcggt cgtgctgcaa ttctgaatat accagcgtaa ccggctggcg
      781 ggtgcgtgtg tagcgggcgc ccttgcgggt gccattgtgt tcagccagac ggcggctcag
      841 atcggtggtg atgccggtgt aataggtgcg gtcactgcat tccaccatat aaacggtcca
      901 ttgggacatg agcaatattt ctcgaaacgg gtttataagt gtttctcaac tatctgatat
      961 atataaatgg ctagcatgaa tcgtggctcg ttggtctgat caaatggtag ccagtgtgaa
     1021 ccgctactat acttcagtgt gctcgcttta acgttcgcaa actgatcgat ttctgcccct
     1081 attgaggcgg ctccattcgc atccggaaga tgacgaaacc atgttaggaa gtcaacgcgg
     1141 cctgccacgc ttgattccgc cgttgttgtg cctgttgttc tgcgtgctgg ccggtttcgc
     1201 accggcttcg ctatcgcagg cgccggtact gacagtgtcg gaggaggttg ggcattaccc
     1261 gcttgcgacc catctggctt atgtccacga cagcaggggc gaatggacca ttgcggatct
     1321 ggtgcgtccg gattccggta tcctgttcca gccgaatcag gaggaaacgc tggcgtttgg
     1381 ttacgtggac gccgtgtact ggctgcgcct ggacgtgaac agcacggacc gcatccgcac
     1441 agcctgggtg ctggaaattg ccgggcttgg ccgtgccatc gacgagattg acctgtacgt
     1501 tcccgatgac gacggccact ggctgcgcca acgctcgggc gattcggtgc cgttctcgca
     1561 gcgttccgtg tcgcatcatg ccgtgctgtt tgcgttccag ttgccctccc gtcagtcgac
     1621 gaccctgtac ttgcgggtga aaaccaacgg caccctgcag attcccgcca cactctggtc
     1681 cagcgcgcgt tatctggaaa acgagcatca cgattcgctg ctggaaggca tcttctacgg
     1741 cgtgctgctg atcatgattt tctacaacgg gtttatttac ctgtcgatcc gtgacaacag
     1801 ctacatcgtc tacgtttgtt atctggtgtg cgtgtttgga ttcgccgtgt cgatcaaggg
     1861 cgtggggttc gaatacctgt ggcccgagca gatatggtgg aacaacaaaa gcaatctggt
     1921 gtttgcagaa ctgggtgtgc tgtttgtgct gctgtttgcc cgcagtttcc tgaatacgcc
     1981 gaaaaacacg cgtctgatca actatgtgat tgcttttctg atcctgacca gcattgcctg
     2041 ctttccggtc gtggtggtgg ccgatcacta catcgcggcg tccgtgctgt cggtgcagta
     2101 tctgctggcg gttgtgtcgg tggtgattgc agcgtcattg tgtctgcagc gcggattcaa
     2161 ggccgcacgc tattacctgc tggcttggct gggtgtgctg gtgtcgatcc tg
//
LOCUS       DASUEG010000664         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_241261, whole genome shotgun sequence.
ACCESSION   DASUEG010000664 DASUEG010000000
VERSION     DASUEG010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241261"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFX11_12530"
     CDS             complement(<1..725)
                     /locus_tag="VFX11_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519007.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX4939495.1"
                     /translation="MAQVIAQWRPEDAGFWQQEGRSIATRNLWISIPCLLLAFSVWMV
                     WSAVTVRLNSIGFAFTTDQLFWLAALPGLSGATLRIFYSFMVPIFGGRKWTAISTASL
                     LIPSIWMGFAVQNPATPFSVFVIIALLCGFGGGNFASSMANISFFYPKSQQGTALGLN
                     AGLGNLGVSLMQFAVPLVITGALFGTIGGAPQSTATGESLWLQNAGFMWVPFLLVCVV
                     AAWFGMNDLAEARASFKEQSVIF"
     gene            complement(756..2063)
                     /locus_tag="VFX11_12535"
     CDS             complement(756..2063)
                     /locus_tag="VFX11_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX4939496.1"
                     /translation="MQHAVSTGNTRILTVSTFTFTVCFAVWTIFAIIGVKIKEELGLN
                     DTQFGLLVAMPILTGSLIRLALGIWTDQIGGRIVLVAVMLAAAASTFLLTTVNTYELY
                     LVAALGVGIAGGAFSVGIAYVSRFYPKEKQGTALGIFGMGNIGAAVTKMLAPLVMVAY
                     GWHAVAQIWAVTLVVTAVLFWFMTEDDPTLQQRRKSGVKAEPFLKQLEPLKNIQVWRF
                     SLYYFFVFGAFVALALWLPRYLIGVYGLDIQTAGLIGACYSIPASIFRVLGGVLSDKY
                     GARRVMYWTFIASVICTFILSYPATEYVVHGVKENIRFELALGVVPFTVVVFVLGFFM
                     SLGKAAVYKHIPVYYPDRVGSVGGVVGMIGGLGGFILPITFGALNDLIGVWTSCFMLL
                     FAIVAVSLVWMHSAVRHLEKQQHPELAGPQDLPEAGALNSARS"
     gene            2365..4224
                     /locus_tag="VFX11_12540"
     CDS             2365..4224
                     /locus_tag="VFX11_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4939497.1"
                     /translation="MITWLKNSLIARGGVAMASIAVIALLNIFASVIVADKTQGDAAA
                     INVAGSLRMQSYKLAYLLQQADSQVSLHRIQQEAALLSGRLHSNELERLLQTHRADAL
                     SAQHDIITSNWHRQIEPMFYDSTLTRERLRDQYDQQVAGFVNDIDTMVRQLQQDSESK
                     IRLLMAIQGASMFLTVIVVFVSMYNLNTSIVIPLRRLLRAAKRVQHGDLQVSLQHDLD
                     DELGQLADAFNQMALELSRMYGDLERKVEEKTAALQRSNQSLQLMYAASRKLAITPYS
                     ASTLGEIIDDLVRTTGVEHVSLCLNENDQRNRFIPLFFSEHEKDKRCMNSSCDECYMH
                     SVRQANFGIDIRDSSFPIRQNRSRFGILFVETRPGEHLAQWQIELFNAVSDTIATALS
                     LEKKAENENRLMLAEERAAIARELHDSLAQSLSYLKFQIGRWKMLQSKAASENQLDEV
                     VEDIRDGLNGAYKQLRELLTTFRLKVDEPGLEPALKGTIAEFSQRGELEIQLDYGLRE
                     FRLTPNEEIHLLQIIRESLSNIIKHAHAKHALIALTATDDKRVRVTIDDDGVGLPKTT
                     HKQHHYGLAIMKERASNLHAELQMVPSPMGGVRVMLDCQLENGPAPTRTVTHV"
     gene            4217..>4524
                     /locus_tag="VFX11_12545"
     CDS             4217..>4524
                     /locus_tag="VFX11_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939498.1"
                     /translation="MSDKTTVLIIDDHPMLRRGVMQLLALDDELEAVGEASSGDEGIK
                     LALALDPDLILLDLNMHGMSGIDTLKALRTADVFSRVLVFTVSDNHEDVVSALKAGAD
                     G"
BASE COUNT         1110 a         1379 c         1218 g          817 t
ORIGIN      
        1 ttgaaaatga cggactgctc cttgaaggag gcgcgcgctt ccgccagatc attcatgcca
       61 aaccaggcgg caaccacgca caccagcagg aatggtaccc acatgaaacc ggcattctgc
      121 agccacaggg attcaccagt agccgtggat tgcggtgcac cgccgatcgt gccaaacagg
      181 gcgccggtaa tcaccagcgg caccgcaaac tgcatcaggc ttacacccag attgcccaac
      241 cccgcattca gtcccagcgc ggtgccctgc tgactcttgg gatagaagaa cgagatattg
      301 gccatgctgg atgcaaaatt gccgccgcca aatccgcaca gcagcgcgat gatcacaaac
      361 acgctgaacg gtgttgccgg gttctgcact gcaaatccca tccagataga agggatcagc
      421 aacgacgcgg tggaaatcgc ggtccacttc cggccaccga aaatcggcac catgaaggaa
      481 tagaaaatcc gcagggtggc accggacaga ccgggcaacg ccgccagcca gaacagctgg
      541 tccgtggtga aggcgaatcc gatggaattg aggcgaacgg ttaccgcact ccacaccatc
      601 cataccgaga aggcaagcag cagacaggga atggagatcc acagattgcg cgtagcaata
      661 ctgcgccctt cctgctgcca gaagccggcg tcctctggac gccattgggc aattacttga
      721 gccatgtcag tctcctcatt ttagaaatag tcaattcagc tgcgcgcgga gttcaatgcg
      781 ccggcctcgg gcagatcctg cgggcctgcc agttccggat gctgttgttt ttccagatgg
      841 cgcacggccg agtgcatcca caccagcgac acagccacaa tggcaaacag caacatgaag
      901 caactggtcc acacgccaat caggtcattg agcgcaccga aggtgattgg caggatgaaa
      961 ccgccgaggc caccgatcat gcccaccacc ccgccaaccg aaccaacgcg gtccgggtag
     1021 taaacgggga tgtgcttgta cactgcggct ttgccaagcg acatgaaaaa gcccagcacg
     1081 aacaccacca cggtgaaggg aaccacgccc agcgccagtt cgaagcggat gttttccttc
     1141 acgccgtgca cgacatattc agtggcgggg taagacagga tgaaggtgca aatcaccgat
     1201 gcaataaagg tccagtacat gacgcggcgc gcaccgtatt tgtcagacaa cacgccaccg
     1261 agtacccgaa agatggaagc aggaattgaa tagcaggcgc cgatcaagcc cgctgtctgg
     1321 atatccagac catacacgcc gatcaggtag cggggtaacc agagcgccag cgctacaaac
     1381 gcgccgaaca cgaagaagta gtagagcgag aaacgccata cctggatatt tttcagaggt
     1441 tcaagctgtt tcaggaaagg ctcggctttc acaccggatt ttctgcgctg ctgcagggtg
     1501 ggatcgtctt cggtcatgaa ccagaacagc actgccgtta ccaccagcgt tacggcccag
     1561 atctgcgcta ccgcatgcca gccatatgcc accatgacca gcggcgccag catcttggtt
     1621 accgccgcgc cgatattgcc catgccgaaa atgcccagcg cggtaccctg cttttccttc
     1681 ggataaaagc ggctgacgta ggcgataccc accgaaaacg cgccacctgc tatccccaca
     1741 cccagcgcag ccaccagata cagttcgtag gtattgacgg tggtcagcag aaaggttgag
     1801 gcggcagccg cgagcatcac tgccaccagc acaatgcgtc cgccgatctg gtcggtccag
     1861 atccccagcg ccagccggat cagagagccg gtcaatatcg gcatggccac cagcaggcca
     1921 aattgcgtgt cgttcaggcc caactcttct ttgattttca caccgatgat ggcaaagatc
     1981 gtccacaccg cgaaacagac cgtgaatgta aacgtgctta ccgtcagtat gcgtgtatta
     2041 cccgtggata ctgcgtgctg cataaaccct ccacactttt ctgattgctt gcggtgaact
     2101 ctagtcagcg cggacgcgga tactattgac aggcatcacc gttttcaaac gtcaaaaatg
     2161 gctataccac cctcatctac caccaaatga gtaaccccaa atccggattt atcctgctgt
     2221 tttagatagc attatttgcg acgtgaaatt tcaggtcgtg aagttgtgct gacagggcag
     2281 cgcaatgtga gtacctacgc gcagagtcga aagttggata caatgaaggc gtgatttcgg
     2341 aaacgagagg aatatggcga cgacatgatt acctggctga aaaattcgtt gatcgcgcgc
     2401 ggcggtgttg ccatggccag tatcgctgtc attgcgctgc tcaacatctt tgcctcggtg
     2461 atcgttgccg acaagaccca gggtgatgcc gctgccatca acgtagcggg gtccctgcgc
     2521 atgcaatcct acaagctggc gtacctgctg cagcaagcgg acagccaggt cagcctgcac
     2581 cgcatccagc aggaagccgc cctgctgagc ggacgtctgc acagcaacga actcgaacgc
     2641 ctgctgcaga cccatcgcgc ggatgcattg tcagcccagc acgacatcat cacgtccaac
     2701 tggcaccgcc agattgagcc catgttttac gacagcaccc tgacccgcga acggttgcgc
     2761 gatcagtacg accagcaagt tgccggcttc gtcaacgata tcgacaccat ggtccgccag
     2821 ctgcagcagg attccgaatc caagatccgt ctgctgatgg ccattcaggg tgccagcatg
     2881 ttcctcacgg tgatcgtggt gttcgtttcc atgtacaacc tcaataccag catcgtgata
     2941 cccctgcgcc ggctgctgcg ggcggccaag cgggtccagc acggtgacct gcaggtatcg
     3001 ctgcaacatg atctggacga cgagctgggc cagctggccg atgccttcaa ccagatggcg
     3061 ctggaactgt cacgcatgta tggcgacctg gagcgcaagg tggaggaaaa gaccgccgca
     3121 ctgcaacgca gcaatcagtc cctgcagctg atgtacgccg cgtcgcgcaa actggcgatc
     3181 acaccctact ccgcctcgac gctgggcgag atcatcgacg atctggtgcg caccaccggg
     3241 gttgaacatg tgtccctgtg cctgaatgag aatgaccagc gcaatcgctt cattcccctg
     3301 ttcttcagcg aacatgaaaa agacaaacgg tgcatgaaca gcagttgcga cgagtgctac
     3361 atgcattccg tccgacaggc caatttcggc atcgacatcc gcgacagctc cttccccatt
     3421 cgccagaacc gcagccgctt cggcatcctc ttcgtcgaaa cccggccggg cgaacatctg
     3481 gcccagtggc agatcgaact gttcaacgcg gtatccgaca ctatcgccac cgcactgagc
     3541 ctggaaaaga aagccgaaaa cgaaaaccgg ctgatgctgg cagaagaacg ggccgccatt
     3601 gcccgcgaac tgcacgattc cctggcacaa tcgctgtcct atctgaaatt ccagatcgga
     3661 cgctggaaaa tgctgcaatc caaggccgcc tccgaaaacc agctggatga agtggtggaa
     3721 gacatacgtg acggtctcaa tggcgcctac aagcaactgc gcgaactgct caccactttc
     3781 cggctgaaag tggacgaacc gggactggag ccggcgctga aaggcaccat cgccgaattt
     3841 tcccagcgcg gcgaactgga aatccagctc gactacggtc tgcgcgaatt caggctcacg
     3901 cccaacgaag aaatccacct gttgcagatc attcgcgaaa gcctgtccaa catcatcaag
     3961 cacgcacacg ccaaacatgc actgatcgca ctgacggcca cggacgacaa acgcgtccgc
     4021 gtcaccatcg acgatgacgg cgtgggcctt cccaagacaa cccacaagca gcatcattac
     4081 ggcctggcca tcatgaagga acgtgcatcg aatctgcacg cggaactgca gatggtaccc
     4141 tcccccatgg gtggggtgcg cgtgatgctg gactgccagc tggaaaacgg acctgcccct
     4201 acacgaacgg tgacccatgt ctgacaaaac aaccgtactt atcatcgatg accatcccat
     4261 gctgcgacgc ggcgtcatgc aactgctggc gctggacgac gaactggagg ctgttggcga
     4321 agcatcctcc ggcgatgaag gcatcaagct ggcgctggca ctggacccgg atctcatcct
     4381 gctggatctc aacatgcatg gcatgagcgg catcgacaca ctgaaagcgc tgcgcaccgc
     4441 cgatgtgttt tcgcgtgtgc tggtgttcac cgtgtccgac aaccatgagg atgtcgtgtc
     4501 cgcactcaag gccggcgccg atgg
//
LOCUS       DASUEG010000665         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1351274, whole genome shotgun sequence.
ACCESSION   DASUEG010000665 DASUEG010000000
VERSION     DASUEG010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351274"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..909)
                     /locus_tag="VFX11_12550"
     CDS             complement(37..909)
                     /locus_tag="VFX11_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939499.1"
                     /translation="MFEIKNCIVDGIRCAYHDSAPQQTAQAVVFVHGNPGPMDDWEQL
                     LPPISRFCRVIAMDMPGFGRSERPSDFDYSIPGYARFLGALLDQLQVQRVHLVLHDFG
                     GAWGLRWVLDHPQRLASLTLINCGIMEGYHWHWMARIWQVPVLGELLQLSFRTQTMQT
                     LVNRANPNPLPAAFSERVRRYADWGHIQAVVKLYRSARDPRDSFPPLQANPDAAKLPV
                     CVIWGAADPFLPVKYAGQQRRYFPAAEVHELAGLGHWPFYDDLQAVLTPLESFLHRQV
                     NALTIKQAEAVTAH"
     gene            complement(979..1377)
                     /locus_tag="VFX11_12555"
     CDS             complement(979..1377)
                     /locus_tag="VFX11_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX4939500.1"
                     /translation="MKKFPLKRYLLGKPEAVEDFPFGPDVAVYRVHGKMFALVSVGGR
                     TESVNLKCHPLEAIELRDVFSAVRPGYHMNKKHWNTVTLDGSIPAGELERMIDNSYAL
                     VVKGLPKNARMGLEVRHGREKIYPAGMLRE"
     gene            1684..2100
                     /locus_tag="VFX11_12560"
     CDS             1684..2100
                     /locus_tag="VFX11_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939501.1"
                     /translation="MINTLLKAYALGMCLLTASILGITCSGPLWEIITELVLHPITDT
                     DLYRYYRMEPLCLNCAGADAAQPLASAPAIPTYAENTLTSTPKVPTLQAAEPIAEEVP
                     PPLVAPLLTRLGIALLAILVGFNHWRFLRYLSQPPA"
     gene            complement(2128..2757)
                     /locus_tag="VFX11_12565"
     CDS             complement(2128..2757)
                     /locus_tag="VFX11_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939502.1"
                     /translation="MEKVFRFTVMMPLLAPLLLASCQSDPPPAATGPAPGSGTITSAP
                     IQVDEKSFKCVRDMTPVRGFFVDNIAGNLEGTLAVANSTTGGVYPPGSVVQLFPNEVM
                     VKRENGFNPITKDWEFFELDVDENGSTIRKRGFQDVNNRFGGNCFACHVQAKPEWDMI
                     CEDSHGCEALPISRHTINSLQNTDPRCEDIDIPFSQRVSAWFIKAFTSF"
     gene            complement(2999..>3579)
                     /locus_tag="VFX11_12570"
     CDS             complement(2999..>3579)
                     /locus_tag="VFX11_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939503.1"
                     /translation="NQRAIAETAREVLKTIKEPQRRAILENFIEHAESECTGDYDRLI
                     ASCSSKSQTYAVYGASEAIAEMQPKTVEEMKDFYRVLVESNVYMIHGEVEKLIVGDHD
                     LYVEIILHQLYPGEVLPMAFGVEFGEEGEVWQLTQRVATVFCFDDENKGCAEHSWTDG
                     PTKPEWLRKEDPRTVPAQFWNNPVTGPRKKPF"
BASE COUNT          748 a         1113 c         1011 g          707 t
ORIGIN      
        1 ccagctggac gcaacagcca tgccagcacg ggacactcaa tgggcggtca ccgcttccgc
       61 ctgtttgatc gtcagggcgt tcacctgccg gtgcagaaac gattccagcg gcgtcagcac
      121 cgcctgcagg tcgtcataaa acggccagtg acccagcccc gccagctcat gcacttccgc
      181 cgccggaaaa tagcggcgct gctggccggc atatttcacc ggcagaaacg gatcggcagc
      241 gccccagatc acgcacaccg gcagttttgc ggcatcagga ttggcctgca acggcggaaa
      301 ggaatcgcgc ggatcgcggg ccgagcggta cagcttcacg acagcctgga tatggcccca
      361 gtcggcgtaa cggcgtaccc gttcgctgaa cgccgccggc aacggattcg gattggcacg
      421 attgacgagt gtctgcatgg tttgcgtgcg aaacgacagt tgcagcaatt cacccagcac
      481 cggcacctgc cagatccgcg ccatccagtg ccagtgataa ccttccatga tgccgcaatt
      541 gatcagcgtc aggctggcca gccgttgcgg atgatccagc acccagcgca agccccaggc
      601 accaccaaaa tcatgcagca ccagatgcac ccgctgcacc tgcaactggt ccagcagcgc
      661 ccccagaaaa cgggcatagc cgggaatgct gtaatcgaaa tcgctgggcc gttccgagcg
      721 gccgaagccc ggcatgtcca tggcaatcac gcggcaaaaa cggctgatgg gcggcaacaa
      781 ctgttcccag tcatccatgg gcccggggtt gccgtgcacg aacaccaccg cctgggccgt
      841 ctgctgcggc gcgctgtcgt gataggcgca gcgaatgcca tcgacaatgc agtttttgat
      901 ttcgaacata ccatctccct tgagtggcca cggcttcgaa tgaaggccgt ttcgagtagc
      961 agcgttccgc gcccgtcctt attcccgcag catgcctgcc ggatagattt tttcccggcc
     1021 gtggcgaact tccagaccca tgcgcgcatt tttcggcaga cctttcacca ccagcgcata
     1081 ggagttgtcg atcatccgtt ccagttcgcc ggcaggaatc gatccgtcaa gcgtcacggt
     1141 gttccagtgc ttcttgttca tgtgatagcc gggacgcacg gcgctgaaca catcgcgcag
     1201 ttcgatggct tccagcggat ggcatttcag gttcaccgat tcggtacggc cacccaccga
     1261 caccagcgca aacatcttgc cgtgcacgcg atacacggcc acgtccgggc caaacgggaa
     1321 atcttcaacg gcttccggtt tgcccagcag gtagcgtttc agggggaatt ttttcatcgg
     1381 acggaggagt tccatggcaa cggcctgact ttattcaagc atatccaggg acatcgtgtc
     1441 gccgcatttg tggcacgtac gtacaacacg tgaaaaccgg cagttgcagg ccaaaaagga
     1501 caatttttac ctgcaaacag ccagcgggga caggtttgct ttactgcgcg ccagccggcg
     1561 tgcaaaatcc ggcttgccgc gttttgtcgg cccgcctttt cgggacggag cgcgcaacgt
     1621 atttcaacca tcaggatgac ctggccaacg ccgggcattc agacgctggg gcagtagaac
     1681 tacatgataa ataccctact caaggcctat gcgctgggca tgtgcctgct gacggcctcc
     1741 atccttggca tcacctgcag tggcccgctg tgggaaatca ttaccgaact ggtactccac
     1801 cccatcacgg atacggatct gtaccgctac taccgtatgg aaccgctgtg cctgaactgc
     1861 gccggtgccg acgctgcaca gccgttagca tccgcgccgg ccattcccac gtatgccgag
     1921 aacacactca ccagtacgcc gaaagtgccc accctgcagg cagcagaacc catcgccgag
     1981 gaggtgccgc cgccactggt tgccccgctg ctgacccgtc tgggcatcgc cttgctggcc
     2041 atcctggtgg gtttcaacca ctggcgcttt ctgcgctacc tgtcgcaacc accggcctga
     2101 gaaataacac ccaggccggc ggctggatca gaacgacgtg aatgccttga tgaaccaggc
     2161 gctgacccgc tgcgaaaatg gaatgtcgat gtcctcgcag cgcggatcgg tgttctgcag
     2221 tgagttgatg gtgtggcggg aaatcggcag tgcctcgcag ccgtggctgt cttcgcagat
     2281 catgtcccac tccggcttcg cctgcacgtg acaggcaaaa caattgccgc cgaaacggtt
     2341 gttcacgtcc tgaaatccgc gcttgcgaat ggtgctgccg ttttcatcca catccagttc
     2401 gaaaaattcc cagtctttgg tgatgggatt gaatccgttt tcgcgcttca ccatgacttc
     2461 gttcggaaac agctgcacca ccgagccggg cggatacacg ccaccggtgg tggagtttgc
     2521 cacggccaac gtgccctcca gattgccggc aatgttgtcg acgaaaaagc cgcgcaccgg
     2581 cgtcatgtcg cggacacact tgaagctttt ttcgtccacc tggatcggag ctgaggtaat
     2641 tgtgccactg cccggcgcag gccctgtggc cgcaggcggc ggatcggact ggcaggatgc
     2701 cagcagcaaa ggcgccagca acggcatcat gacagtgaag cgaaaaacct tttccatacc
     2761 cttctcccga tgatcaaaaa cagcaatacg ccggcatcag acccaatcaa gccggcgaca
     2821 caaaacgtgc aatttgtatt gtctggattt ggaaacggcc tcgccatggt caaacccgac
     2881 acaggtgggc ggataggacg cgaacgaaga caggaatgga aaaagctttt tcagtaaaac
     2941 aggaaaacac ccgccaccgg aacgatggcg ggtgctggaa acaatggaag cggcgggatc
     3001 agaacggttt tttgcgcgga ccggtcaccg ggttgttcca gaactgcgcc ggcaccgtgc
     3061 gcggatcttc cttgcgcagc cactcgggtt tggtggggcc gtcggtccag gaatgctcgg
     3121 cgcaaccctt gttttcatcg tcgaagcaga acacggtggc cacgcgctgg gtcagctgcc
     3181 acacctcgcc ttcctcgccg aattccaccc cgaacgccat cggcagcact tcgcccggat
     3241 acagctggtg caggatgatt tccacataca ggtcgtgatc gcccacgatc agcttttcga
     3301 cttcgccgtg aatcatgtaa acattgcttt ccaccagcac gcggtagaaa tccttcattt
     3361 cctcgacggt tttcggctgc atttcggcta tggcttccga cgcaccgtac acggcgtagg
     3421 tctggctctt ggacgagcag ctggcgatca ggcggtcgta atcgccagtg cactcggatt
     3481 ccgcgtgctc gataaagttc tccaggatcg cccggcgttg gggttccttg atggttttca
     3541 gcacttcccg cgcggtctcg gcgatggcgc gctggttga
//
LOCUS       DASUEG010000666         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_427413, whole genome shotgun sequence.
ACCESSION   DASUEG010000666 DASUEG010000000
VERSION     DASUEG010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427413"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1400
                     /gene="flgE"
                     /locus_tag="VFX11_12575"
     CDS             96..1400
                     /gene="flgE"
                     /locus_tag="VFX11_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019440866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX4939504.1"
                     /translation="MSFNIALSGLNAAQAQLDVTSNNIANVETTGFKQSRTMFGDIYA
                     NSTFGNSDTAIGNGVLLQDVTQLFEQGNLDFTSQALDLAISGEGFFVMSPDQISQERI
                     YSRAGAFGVDSNGFVVNAAGQFLQVFPVNANGTVTATALSSTTPLQLPDTAGAPQATN
                     EVEIGVNLPATATDLAGVAFDPAQPNTYTASTSITLYDSLGASHIATAYFAKDSSVGA
                     GTNSWRVHLGITDDTGTIQPIALDDGAGGTTPYGILEFNQQGIFTGTTPTVIRTDQFG
                     GLLTNGADPTQQVVFDFSGNTPTQFASPFTVNTLSQNGFTIGRLTGLNVNEQGVVQAT
                     YSNGQTESVGKVALARFSNPQGLLQIGNTSWRASTSSGEPIPGEAGTSSFGLIQSGAL
                     ETSNVDLTKELVTLITAQRNFQANAKSIETSNAITQTIIQIR"
     gene            1520..2257
                     /locus_tag="VFX11_12580"
     CDS             1520..2257
                     /locus_tag="VFX11_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HEX4939505.1"
                     /translation="MDKLLYVSMTGAKEALDAQRLRANNLANASTDGFKADLHQYRSM
                     SVFGEHFPSRAYAMGERPGTDMSAGPLIETGRELDVAVKGNGWLAVQMPDGTEAYTRA
                     GDLQRTVSGLVTTGTGLPVMGDGGPVVLPEYETLLVGVDGTLSIRGLGEQPMAMTQVA
                     RLKLVNADPRQLVKGEDGLFRTRDGAPLEPSADVQVESGMLEGSNVNPVSELTAVISH
                     ARLFDMNVRMMKTAQENDEAAARILQG"
BASE COUNT          517 a          656 c          688 g          502 t
ORIGIN      
        1 gataaccagg gcagcatgat tctgaatctg aacggtattg gccagatcgc atcgaccgat
       61 atccaagaag tacgttaatt ctgcaggagt agagtatgag ttttaacatt gcgctgagcg
      121 gactgaatgc ggcccaggcc caactggacg tgaccagcaa caatattgcc aacgtggaaa
      181 ccaccggctt caagcaatcg cggaccatgt ttggtgatat ctatgcgaat tccacctttg
      241 gcaacagcga taccgcaatc ggtaatggcg tgttgctgca ggatgtgacc cagttgtttg
      301 agcagggcaa cctggacttt acgtcccagg cgctggatct ggcgatcagc ggcgaaggct
      361 tttttgtcat gtcgccggac cagatcagcc aggagcgcat ttacagtcgc gccggtgcat
      421 tcggtgtgga cagtaatggt ttcgtggtga atgcagccgg acagtttctg caggtatttc
      481 cggtcaacgc gaatggcacc gtaacggcca ctgcgctgtc gtccaccaca ccgctgcagt
      541 tgccggacac cgctggtgcg ccgcaggcca ccaatgaagt cgaaatcggc gtcaacctgc
      601 cagccactgc aaccgatctg gccggcgttg cattcgatcc ggcccaaccc aatacctaca
      661 ctgcatcaac gtccatcacc ctgtatgaca gtctgggtgc ttcgcacatt gcaacggcct
      721 atttcgccaa ggattcctcg gttggcgccg gtaccaacag ctggcgcgtg cacctgggca
      781 ttaccgacga caccggcacc attcagccca tagcgctgga cgatggcgct ggcggcacca
      841 caccctatgg cattctggaa ttcaaccagc agggtatttt taccggcacg acgccaaccg
      901 ttatccgcac tgaccagttt ggcggtctgc tgaccaacgg cgctgaccct acccagcagg
      961 tcgtgttcga tttcagcggc aatacgccca cccagtttgc atcgcctttc accgtcaaca
     1021 cgctgtcgca aaatggtttc accatcggac gtctgaccgg cctgaacgtg aacgaacagg
     1081 gtgtggtgca ggctacgtat tccaatggcc agaccgaatc cgtcggcaag gttgcattgg
     1141 cacgcttttc caacccgcaa ggtttgctgc agataggaaa caccagctgg cgtgcttcca
     1201 cgtcctctgg tgagccgatt ccaggtgaag ccggtacgtc cagttttggc ctgattcaat
     1261 ccggcgccct ggaaacctcg aacgtcgatc tgaccaagga gctggtaacg ctgattactg
     1321 cgcagcggaa tttccaggcc aacgccaaat cgatcgagac ttccaacgcc attacccaga
     1381 ccatcatcca gatccgctga ttccttctcc tcctcttttt gagccccgcg attgcggggc
     1441 ttttttttgg caagtgattt gcacttcact gttcatcggc gttagacgct gaaattttga
     1501 caggcgatac aggtgtccca tggacaaact tctttatgtt tcgatgacag gcgcaaaaga
     1561 ggcgctggat gcacagcgtc tgcgtgccaa caatctggcg aacgcgtcca cggatgggtt
     1621 caaggccgat ctgcatcagt accgttccat gtcggtgttt ggtgagcact ttccctcgcg
     1681 tgcctatgcc atgggtgagc ggccgggtac ggacatgagt gcgggcccgc tgatcgaaac
     1741 cgggcgtgaa ctggatgttg cggtgaaagg caatggctgg ctggcggtgc agatgcctga
     1801 cggaacggaa gcttacaccc gcgccggtga tttgcagcgc acggtgagcg ggctggtgac
     1861 aacaggaaca ggtcttcccg tgatgggcga cggtggcccg gtggtgttgc cggaatatga
     1921 aacgctgctg gtgggtgttg atggcacgct gtcgattcgc ggattgggcg aacagcccat
     1981 ggcgatgacg caggtcgcgc ggctgaaact ggtaaatgca gatccgcgcc agctggtgaa
     2041 aggagaggac ggactgttcc gtacccggga cggcgcgccg ctggagcctt ccgcggatgt
     2101 gcaggtggaa agcggcatgc tggaaggttc caacgtcaat ccggtcagcg aattgacggc
     2161 ggtaatctcg cacgcgcgcc tgtttgacat gaatgtgcgc atgatgaaaa cagcacagga
     2221 aaatgacgaa gcagcggcac ggatactgca aggttgacag cagtgtcgaa aaaacgccgc
     2281 gcctgatgtc atagcggcaa attttttccc gatccggatg cagattgccg gacgaagaac
     2341 tggagtagtg tatgcactca tcc
//
LOCUS       DASUEG010000667         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1467122, whole genome shotgun sequence.
ACCESSION   DASUEG010000667 DASUEG010000000
VERSION     DASUEG010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467122"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFX11_12585"
     CDS             <1..426
                     /locus_tag="VFX11_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX4939506.1"
                     /translation="AKRLGARKVMALINNPAYVDLVQGGEIDIAISPQQATIGSLLTH
                     VRRGDVANVHSLRRGAAEAIEAIAHGDEKSSKVVGKAIGDIDLPEGTTIGAIVRKNEV
                     LIAHDDIVVQSEDHVILFLIDKRRIQDVERLFQVGITFF"
     gene            442..1893
                     /locus_tag="VFX11_12590"
     CDS             442..1893
                     /locus_tag="VFX11_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX4939507.1"
                     /translation="MRPAIILRIFGQLLMIYSGTLLVPVLVAALYGEADMLRFLYSFL
                     ATLLSGLLCWLPVQNRRRELRVRDGFIIVTLFWSVLSLFGALPYLLGQDLRLAFTDAL
                     FESVSGFTTTGATVFTGLDQLPKSLLYYRHQQQWLGGLGIIVLAVAILPMLGVGGMQM
                     YRAETPGAVKDSKLTPRVTETARSLWYIYVALTVACALGYWLCGMDLFDAICHSFSTV
                     SIGGFSTHDASLGFYNSAAIEAVAIVFMLLAGMNFGLHFIAWRRRSLRQYATDGEVRF
                     YFFVVAGVSALVFIGLLTTTGMADPALSLRHALFESVSVITTTGFGVAQYASWHPMLA
                     TALIMASFIGACAGSTGSGLKMVRVNLMFKQGLREVRRLMHPKAMILVKLSNRAVPDR
                     VVEAVWGFFGIYLLTFCVLFLLLLLTGMDFLSGFSAVAACLNNLGPGLGDVTLHYAAV
                     SDPAKWILCAAMLLGRLEIFTLLVLFTPMFWRH"
     gene            2013..3002
                     /locus_tag="VFX11_12595"
     CDS             2013..3002
                     /locus_tag="VFX11_12595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939508.1"
                     /translation="MFNHDEWILEQQNPMISLKKWQTTVDLMARLYEAPTAYVSQYTS
                     RGFQTVIANSNSANPYKAGDTFGRDTNLFCRKVVQDCAQVYENHATSNTVWRGNPMME
                     NNRFNSYLGVPIYWPDGTPFGTICVLDYAVTHYNKNFLELIWQFRDLVEADLMLNNQF
                     MQLLELSTRDDLSRLLNRRGFFSQADKQVRLASRVKQNIGVLYLDLDNLKKINDQMGH
                     RIGDKAIAALATAIRKVIRESDVAGRIGGDEFVIVMLAEDEEALAKLTYRIRQELKHQ
                     AVGELQSIELSVSIGSRLFRHPHLSSIDKMVSEVDQRMYEDKLANHRGLGRKA"
BASE COUNT          605 a          966 c          945 g          696 t
ORIGIN      
        1 gccaaacggc tgggggcacg caaggtcatg gcgctgatca acaacccggc ttatgtcgat
       61 ctggtacaag gcggcgagat cgatatcgcc atctcccccc agcaggccac catcggcagc
      121 ctgctcaccc acgtgcggcg cggtgacgtg gcgaacgtgc actcgctgcg gcgcggcgcg
      181 gcggaggcca tcgaggcgat tgcccacggc gacgaaaaat cctcgaaggt ggtgggcaag
      241 gccatcggcg acatcgatct gccggaaggc accaccatcg gcgccatcgt gcgcaagaat
      301 gaagtcctca tcgcccacga cgacatcgtg gtgcagtcgg aagatcacgt gatcctgttc
      361 ctgatcgaca agcgccgcat ccaggatgtc gagcgtctgt tccaggtcgg catcacgttt
      421 ttctgacgct ggatacgctg catgcgtccc gccatcatcc tgcgaatttt cggccagctg
      481 ctgatgatct acagcggcac gctactggtg ccggtgctgg tggcggcgct gtatggtgaa
      541 gccgatatgc tgcggtttct gtattccttc ctggcgacat tgctaagcgg attgctgtgc
      601 tggctgccgg tgcagaaccg gcgccgggaa ctgcgggtac gcgacggttt catcatcgtg
      661 acgctgttct ggagtgtgct gagcctgttc ggcgcgctgc cctatctgct ggggcaggat
      721 ctgcgcctgg ccttcaccga cgcgctgttc gaatccgtgt ccggcttcac caccaccggt
      781 gctaccgtgt tcaccggcct tgaccagttg ccgaagtcgc tgctctatta tcgacaccag
      841 cagcagtggc tgggggggct gggcatcatc gtgctggcgg tggcgatcct gcccatgctc
      901 ggcgtcggcg gcatgcagat gtaccgggcg gaaacgccgg gcgcagtgaa ggacagcaag
      961 cttacgcccc gggtgaccga aacagcgcgc tcgctctggt acatctatgt cgcgctcacc
     1021 gtcgcctgcg cgctgggcta ctggttgtgc ggcatggatc tgttcgacgc catctgtcac
     1081 agtttttcca cggtttccat cggcggcttc tccactcacg acgccagtct gggtttctat
     1141 aacagcgccg ccatcgaagc agtggccatt gtgttcatgc tgctggccgg catgaatttc
     1201 ggcctgcatt tcatcgcctg gcgccgccgc agcctgcgcc agtacgccac cgacggcgaa
     1261 gtgcggttct atttttttgt ggtggccggc gtgagcgccc tggtgttcat cggcctgctg
     1321 accaccaccg gcatggccga tccggccctg tcattgcgcc acgccctgtt cgaatccgtc
     1381 tccgtgatca ccaccaccgg ttttggcgtg gcgcagtacg ccagctggca cccgatgctg
     1441 gccaccgcgc tgatcatggc cagctttatc ggcgcctgtg ccggttccac cggcagcggg
     1501 ctgaaaatgg tgcgggtgaa cctgatgttc aagcagggcc tgcgcgaagt gcgccggctg
     1561 atgcacccca aggccatgat tctggtgaaa ctgtccaacc gggcggtacc ggaccgggtg
     1621 gtggaagccg tctggggatt tttcggcatt tacctgctca cgttctgtgt cctgttcctg
     1681 ctgctgctgc tcaccggcat ggattttctg tccggctttt ccgctgtcgc ggcctgtctc
     1741 aataacctgg gacctggact gggggatgtc accctgcatt atgctgcggt gagcgacccc
     1801 gccaaatgga ttttgtgtgc cgccatgtta ttggggcggc tggaaatatt caccctgctg
     1861 gtgctgttca cgccgatgtt ctggcgccac tgaagaacgg acattgttcg caaaacaaaa
     1921 tcgaattccg ttaattttgc gcatttcgcg gtttccggtt ttattactga ctatactcat
     1981 gtctattctt tccccactct gtatcatcgg ccatgttcaa tcacgacgaa tggattcttg
     2041 aacaacagaa tccgatgatt tcactgaaaa aatggcagac gaccgtggac ctgatggcgc
     2101 ggttgtatga agcgcccact gcctatgtct cgcagtacac ctcgcgtggc ttccagaccg
     2161 tcatcgccaa ctccaattca gccaatcctt acaaggccgg tgatacgttc gggcgtgaca
     2221 ccaacctgtt ctgtcgcaag gtggtgcagg actgcgcgca agtgtatgaa aatcatgcca
     2281 ccagcaatac ggtctggcgc ggcaatccga tgatggagaa taaccgcttc aattcctatc
     2341 tgggcgtgcc catctactgg cccgacggca cacccttcgg caccatttgc gtgctggatt
     2401 atgcagttac ccactacaac aagaatttcc tggaactgat ctggcagttc cgtgacctgg
     2461 tggaagcgga cctgatgctg aacaaccagt tcatgcagct gctggagctg tccacccgcg
     2521 acgatctcag ccggctgctc aaccggcgcg gttttttttc ccaggccgac aagcaggtgc
     2581 gcctcgccag ccgcgtgaag cagaacatcg gcgtgctgta tctggacctg gataatctga
     2641 aaaaaatcaa cgaccagatg ggacaccgca tcggcgacaa ggccatcgcc gcactggcca
     2701 cggccattcg caaggtgatc cgtgaaagcg acgtggcggg ccgcattggt ggcgacgaat
     2761 tcgtgatcgt gatgctggcc gaagacgagg aagcattggc caaactcact taccgcatcc
     2821 gccaggaact gaagcaccag gcggtgggtg aactgcagtc gattgaactg agtgtgagca
     2881 tcggcagccg cctgttccgt catccgcacc tgagcagtat cgataaaatg gtgtcggaag
     2941 tggatcagcg catgtacgag gacaagcttg ccaatcaccg tgggttgggc aggaaagcat
     3001 aatcggccga gcgccggcgc ttgcacccgg ccgatctgtg ctgtcactgc aacctggcgg
     3061 ccgttccgaa tccggctttg acggttgacg aacctgagac gcgcacttca gatgaacggt
     3121 gccggcttca ccgcggtgcc cccatccagc gcaatcacgt tgccggtaat gtacgatccc
     3181 gccggcgacg tcagaaatac aatcaaccct tc
//
LOCUS       DASUEG010000668         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1659694, whole genome shotgun sequence.
ACCESSION   DASUEG010000668 DASUEG010000000
VERSION     DASUEG010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659694"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFX11_12600"
     CDS             <1..524
                     /locus_tag="VFX11_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010567245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4939509.1"
                     /translation="NITALRGTVDLDTRPGQGTTFRVRMPLTLAIIDGFQVGVEKSSY
                     VVPLDMVLECVELNEAANGNATERDYLNLRGEVLPFIRMRDLFAVSGQPPRRENVVVV
                     QYGGNKAGLVVDQLLGEFQTVIKPLGKIFSHVRGISGSTILGNGEVALIIDVPDLVQH
                     VSTLEYQAARARA"
     gene            555..2165
                     /locus_tag="VFX11_12605"
     CDS             555..2165
                     /locus_tag="VFX11_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4939510.1"
                     /translation="MFNNMKIGMRLGISFAIVLLLMTVIIGTGAEGLMSVNKITDRIV
                     TQDWTKAVVANEIVDLANDNARASFEIFLLRDPAMIAATKDRISNNVNTINDKVALLE
                     KLIYAEEGKKLLATMKEVRKPYVASFKTVVKLLDEGRNEEASALMISQTLPALNIFMG
                     AINDLIQLQGRLMEKSGQEAAATYLNGRNLMIAVGLAALAIGAGAAFWVTRSITRPVN
                     VAVAVANQLANGDLSARIDVTGKDEVGQLLVAMQNMVGKLAQIIGEVRGAASALSSAS
                     EEVSATAQSMSQGSSEQAASVEETSASVEQMSASISQNTENAKITDGMASKAAREADE
                     GGRAVKETVQAMKIIADKVGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVR
                     KLAERSQVAAQEIGEVAQSSVNLAEKAGKLLDEIVPSIKKTSDLVQEISAASEEQSSG
                     AAQINIAMEQLNKVTQMGASSSEELAATAEEMSSQAEQLQQLMGFFKLEIAATNAAVA
                     RQDAPRKKAHAERPQSPVALNSSEFVSF"
     gene            2176..2715
                     /locus_tag="VFX11_12610"
     CDS             2176..2715
                     /locus_tag="VFX11_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011704963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4939511.1"
                     /translation="MNLSSMMVASNGATPRTGHPQESQYLTFLLRNEMFAIGILNVKE
                     ILEYGMLTSVPMMPEFIRGVINLRGSVVPVVDLGARFGGRPCDIGKRTCIVIMEVSSD
                     DGHQDIGVVVDSVSAVLDIPAGDIEPAPAFGARIRADFIDAMGKIDGKFVIILNVGKV
                     LSVDELAMLGQVSETAEAG"
     gene            2728..3534
                     /locus_tag="VFX11_12615"
     CDS             2728..3534
                     /locus_tag="VFX11_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX4939512.1"
                     /translation="MDHPVLRDSEFTLFQKMMYDITGIHLTDVKKTLVSGRLAKRVKQ
                     LGMASYGDYFTLLKKPGSTEMQIAVDLLTTNETFFFREQKHFDFLRDRVLPQWDGGMR
                     RLWSAASSSGEEAYTLAMVMAEHARTDTWEIVGTDISSRVVEKAQRGQYPLERAENIP
                     RPLLTRYCLKGVGSQDGTFLIADTLRKRTRFCHANLKGDLSALGSFDIIFLRNVMIYF
                     DMPTKQQIVARMIQQLKAGGYLMVGHSESLNGVSGALKPVAPSIYQKPLP"
     gene            complement(3578..>4188)
                     /locus_tag="VFX11_12620"
     CDS             complement(3578..>4188)
                     /locus_tag="VFX11_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4939513.1"
                     /translation="LIVPIGEWVLRTASAQAQHWQTTFQRNISVAVNISARQFRTCDL
                     VSLVQQVLRDTGLHPGALELELTESMLMHDVKRVQQVLTGLRGLGVSLAVDDFGTGFS
                     SLNYLKSFPLDALKIDRSFVTDIEQGANDEAIVRAIIALAHSLNLRVVAEGVETAAQM
                     RYLLKQGCDEVQGYYFSRPQTLDQISLMLQQQSVLELAQLRA"
BASE COUNT          936 a         1231 c         1261 g          760 t
ORIGIN      
        1 gcaacattac cgcactgcgt ggcaccgtcg acctggacac cagaccgggc cagggcacca
       61 ccttccgggt gcgcatgccg ctgaccctcg ccatcatcga tggcttccag gtgggcgtgg
      121 aaaaatcctc ctacgtggta ccgctggaca tggtgctgga gtgcgtcgaa ctcaatgaag
      181 cggccaacgg caacgcgacc gagcgcgact acctcaatct gcgcggcgag gtactgccct
      241 tcatccgcat gcgcgacctg tttgccgtga gtggccagcc accgcggcgg gaaaatgtgg
      301 tggtggtgca gtacggcggc aacaaggccg gtctggtggt ggaccagttg ctgggagaat
      361 tccagaccgt gatcaaacca ctgggcaaaa tattcagcca cgttcgcggc atcagcggct
      421 ccaccattct tggcaatggc gaagtggcgc tgatcatcga cgtgccggat ctggttcagc
      481 atgtatcaac actggaatat caggccgccc gtgcgcgcgc ctgacattgc atcgcacaac
      541 ctgaggagaa aacaatgttc aacaacatga aaataggtat gcggcttggc atcagcttcg
      601 caatcgtgtt gctgctgatg actgtcatca ttggcaccgg cgcagaaggg ctcatgtcgg
      661 tcaacaaaat aaccgaccgc atcgtgaccc aggactggac caaggccgtg gtagccaacg
      721 aaattgtgga tctggccaac gacaacgccc gcgccagctt cgagattttt ctgctgcgcg
      781 atccggcgat gatagcggcc accaaggatc gcatcagcaa caacgtcaac accatcaacg
      841 acaaggtggc gctgctggaa aagctgatct atgccgagga aggcaagaag ttgctcgcca
      901 ccatgaagga agtgcgcaaa ccctatgttg catccttcaa gaccgtggtg aaactgctgg
      961 atgaaggccg taacgaggaa gccagcgcgt tgatgatcag ccagaccctg ccagccctga
     1021 atatcttcat gggcgccatc aacgacctga ttcaactgca gggtcgtctg atggaaaaat
     1081 ccggccagga agcagcagca acgtatctga atggtcgcaa cctgatgatt gccgttggcc
     1141 tcgcggcact ggcgatcggc gccggtgctg ccttctgggt gacccgttcc atcacccgcc
     1201 cggtcaatgt ggcggtggca gtggcgaatc agctggccaa cggcgatctg tcggcgcgca
     1261 tcgatgtcac cggcaaggat gaggtcggcc aattgctggt cgccatgcag aacatggtgg
     1321 gcaagctggc gcagatcatc ggcgaagtgc gcggcgccgc ctcggcactg tcatccgcat
     1381 cggaggaagt gtcggccacc gcgcagtcca tgagccaggg ctccagcgaa caggccgcca
     1441 gcgtggaaga aacgtcggcc tcggtcgaac agatgtcagc ttccatcagc cagaataccg
     1501 aaaatgccaa gatcaccgat ggcatggcat ccaaagcggc acgcgaagcg gatgaaggcg
     1561 gccgcgccgt gaaggaaacc gtgcaggcca tgaagatcat tgccgataag gtgggcatca
     1621 tcgacgacat cgcctatcag accaatctgc tggcactgaa tgccgccatc gaagcggcgc
     1681 gcgccggcga gcatggcaag ggctttgccg tcgttgcggc ggaggtacgc aagctggccg
     1741 aacgctcaca ggtcgccgcg caggaaatcg gagaagtggc ccagagcagc gtgaacctgg
     1801 cggaaaaagc cggcaagctg ctggatgaga tcgtgccgtc catcaagaaa acatcggatc
     1861 tggtgcagga aatcagcgcc gcgtcggaag aacagtcctc tggcgcagcc cagatcaata
     1921 ttgcgatgga acagctcaac aaggtgaccc agatgggtgc gtcctcgtcg gaagagctgg
     1981 ccgccaccgc cgaggaaatg agtagccagg ctgaacagct gcagcagctg atgggctttt
     2041 tcaagctgga aatcgcggcg accaacgccg ccgttgccag acaggatgcg ccgcgcaaga
     2101 aagcccatgc agaacggccg caatcgccgg ttgcgctgaa ttcctccgaa ttcgtcagct
     2161 tctaggaggc agttcatgaa cctgtcatcc atgatggtgg cgtcgaatgg cgccacccca
     2221 cgcacgggcc acccgcagga aagtcagtac ctgactttcc tgctgcgcaa cgaaatgttt
     2281 gccatcggca ttctcaacgt caaggaaatc ctcgagtacg gcatgctgac cagcgtgccg
     2341 atgatgccgg agttcatccg cggtgtgatc aacctgcgcg ggtccgtggt accggtggtg
     2401 gatctgggcg cgcgcttcgg tggcaggccc tgcgacatcg gcaagcgcac ctgcatcgtc
     2461 attatggaag tgagcagcga cgacggacac caggacattg gcgtggtggt ggattcggtg
     2521 tcggccgtgc tggacattcc cgccggcgat atcgaaccgg cgccagcctt tggcgcgcgc
     2581 attcgcgctg atttcatcga cgccatgggc aagatcgacg gcaaattcgt gatcatactc
     2641 aacgtgggca aggtgttatc ggtggatgaa ctggccatgc tgggccaggt cagcgaaacg
     2701 gccgaggccg ggtagggttg acacggcatg gatcatcccg tcctgaggga cagcgaattt
     2761 acgctgttcc agaaaatgat gtacgacata accggcattc atctgacgga cgtgaagaag
     2821 acactggtga gcggacgcct ggcaaagcgg gtcaagcagc tgggcatggc cagttacggt
     2881 gattatttca ccctgctgaa aaagccgggc agcaccgaga tgcagatcgc tgtcgacctg
     2941 ctcaccacca acgaaacgtt cttcttccgc gagcagaagc attttgattt tctgcgcgac
     3001 cgcgtgctgc cgcagtggga tggcggcatg cgccggctgt ggagcgccgc cagttcatcg
     3061 ggcgaggaag cctacacgct ggcgatggtg atggcggaac acgcgcgcac ggacacctgg
     3121 gaaattgtgg gcaccgacat cagctcccgg gtggtggaga aagcccagcg gggtcagtat
     3181 ccactggaac gggcagagaa cattccacgc ccgctgctca cccgctattg tctcaaaggc
     3241 gtgggcagcc aggacggcac ctttctgatt gcggacaccc tgcgcaagcg cacccgtttc
     3301 tgtcacgcca acctgaaagg tgatctcagc gctctgggca gtttcgacat catcttcctg
     3361 cgcaatgtga tgatctattt tgacatgccc accaagcagc agattgtcgc gcgcatgatc
     3421 cagcaactca aagcgggcgg ctatctgatg gtggggcatt cggaaagcct gaacggcgtg
     3481 tccggcgcac tgaaaccggt ggctccgtcg atctaccaga agccactgcc ctgaaaatcc
     3541 agctctgaac atcctgcgtg aaaactggtg gccaacatca ggcccgcaat tgcgccagtt
     3601 ccagtacgga ttgctgctgc agcatgaggc tgatctggtc cagggtctgc ggccggctga
     3661 aatagtagcc ctgcacttca tcacacccct gtttcagcag ataccgcatc tgcgccgcgg
     3721 tctccacccc ttccgccacc acgcgcagat tcaggctgtg tgccagtgca ataatggcgc
     3781 ggacaatggc ttcgtcgttg gcaccctgct caatgtcggt gacaaaggaa cggtcgatct
     3841 tgagcgcatc cagcggaaaa cttttcaggt agttcagcga agaaaagcca gtgccaaaat
     3901 catccaccgc cagacttaca cccagcccgc gcaatccggt cagcacctgt tgcacccgtt
     3961 tcacatcatg catcagcatc gattctgtca gttccagctc cagcgcaccc ggatgcaatc
     4021 cggtgtcgcg cagcacctgc tgcaccaggc tgaccagatc gcaggtacgg aactggcggg
     4081 ccgagatgtt gaccgccaca ctgatattgc gctggaacgt cgtctgccag tgttgcgcct
     4141 gcgcgctggc ggttcgcagc acccactcgc caatgggcac gatcaggc
//
LOCUS       DASUEG010000669         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_367719, whole genome shotgun sequence.
ACCESSION   DASUEG010000669 DASUEG010000000
VERSION     DASUEG010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367719"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VFX11_12625"
     CDS             <1..1817
                     /locus_tag="VFX11_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4939514.1"
                     /translation="VGTVPVMLKLGSEFMPPLDEGSLLVMPTTFPGISIEQARRALNA
                     QDRIIKSFAEVESVHGKTGRAETATDPAQLDMNEIVVTLKLRDEWPLFETSRWYSSWA
                     PEWLKRALRPVWPEAQPRTQQELVRALDAALKMPGYQMAIAPPIRTRIDMLTTGVRTP
                     VGIKVFGADLAEIERLSIELEGLLREVPGTRSTFAERQTGREYVDIIPDREAIARYGL
                     TVRDTLDVVEAAVGGMPVSTVIDGRARYSVSLRYAADFRADPEALRALPVQVPGMAAI
                     AEPEIGGSGRGSSAAPAMSGGGSGGMAMGGGGARRSAGAASMPASSAPMGAVTEPDVA
                     ERFRQAGTAVPLGALADVRVVTGPPMIKDENGVLVGYVFADIDDTQRDLGGWVDDAKQ
                     LVSQRFALPPGYSLQWTGQYEFMADMQARMRVVLPLTFVLIVVLLYLSMRGVQQTLLV
                     LLSLPFAIAGSIWLLALLDYNLSTAVWVGLIAVGGVAAQTGIVVVVYLDQAFQARVES
                     GNLRQPGDVDDAVIEGAAKCLRPMLMTVATTVLGLMPLLWEAGVGADLSARTAAPVVG
                     GLWACMFLSLLVLPATYTFWRRYQFGRGNFNLREMPNE"
     gene            1810..>4137
                     /locus_tag="VFX11_12630"
     CDS             1810..>4137
                     /locus_tag="VFX11_12630"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX4939515.1"
                     /translation="MNNGMASTKTPIGLSSSEAAGLLASVGPNEIQHAAVVPPWLLLA
                     GQFKSPVIWLLLGACVVSAVLGEATNAIAIGSIVVINAFVGFFQEYRAERAVNALRSM
                     TAPRANVLRDGMMKVIPAMEVVPGDILLVEAGDVVAADAHLLEAHSLSVNEAALTGES
                     QPSEKSILPVATNAPLAERHDSIFLGTAVVSGTAVAKITATGMATELGKIAHLLASVQ
                     DEATPLQQRLARVSKSLLFICLGIVSAVAILGVVRGTPLLEVFLSAVSLAVAAVPEGL
                     PAVVTIALAVGVQRMAARKVLVRRLPAVETLGCTTVICTDKTGTLTTGVMAVRELWGA
                     DHVRLLDVAAACCNGELAQDERSGVGDTTEVALLMAAAARGIHRKDIEQKRPRVMENP
                     FDSERKRMSILRSDGVLYVKGAVDLLLPLCRGDNSAALAANADMSSRGLRVLAMAVGK
                     GREENELELVGLTGIADPPRTEAIAAIASARSAGIRTVMITGDHPITAQAIALELGLL
                     QKGEAAQELVHARATPEDKLKIVKKFKEQGAIVAMTGDGVNDAPALREAHIGIAMGKN
                     GTEVTREAADVVLSDDNFASIVAAVEEGRGIFDNIRKTLVYLLAGNAGELMLMFSAAL
                     LGLPLPLLPLHLLWINLVTDGLPALALVTDPLDRNVMKRPPRDPREPILGKPQWRRIL
                     VSGLLQASVALAVFVWALENRDLNEARNLAFTTLVFAELFRAFSARSSLPFWEVGIFT
                     NMRLFGIVVVSVVVQLGIHHIPATQALFEIHDLSLE"
BASE COUNT          796 a         1071 c         1339 g          931 t
ORIGIN      
        1 tggtcggcac cgtgccggtc atgttgaagc tgggatcgga attcatgccg ccgctggatg
       61 aaggttccct gctggtgatg ccgacgacct ttcccggcat ttccatcgag caagccagac
      121 gtgcgctcaa cgcacaggat cgcatcatca agagttttgc cgaagtggaa tccgtgcatg
      181 gcaaaaccgg acgggcggaa acggcaaccg atccggccca gctggacatg aatgaaatcg
      241 tggtcacgct gaaactccgt gacgaatggc cgctgtttga aacgtcgcgc tggtacagca
      301 gctgggcgcc cgagtggctg aaacgcgcat tgcgaccggt gtggccggag gcgcagccgc
      361 gcacccagca ggaactggtg cgtgcgctgg atgcggccct gaaaatgccg ggttatcaga
      421 tggccattgc accgccgatc cgtacccgca tcgacatgct gaccaccggc gtgcgcacgc
      481 ctgtcggcat caaggtgttt ggtgctgatc tggcggaaat cgaacggctg tcgatcgagc
      541 ttgaaggttt gctgcgggaa gtccccggta cgcgcagtac atttgccgaa cggcagacgg
      601 ggagggaata cgtcgacatc attccggatc gcgaagccat cgcccgctac ggcctcacgg
      661 tgcgcgacac gctggatgtg gtggaagccg ccgtgggcgg aatgccggtg tcgacggtca
      721 ttgatggccg cgcccgctat tcggtgagcc tgcgttatgc agcggatttt cgtgccgacc
      781 cggaagcttt gcgggcgttg ccggtgcagg ttcccggtat ggctgcgatt gctgaacctg
      841 agattggcgg atctggtcgt ggtagcagcg ccgctcctgc gatgagtggc ggtggcagtg
      901 gcggcatggc aatgggtggc ggcggtgcgc gccgttctgc tggtgcggct tcgatgccag
      961 cttcatctgc gcccatgggg gcggtgacag aaccggacgt ggctgagcgt tttcgccagg
     1021 ccggcaccgc ggttccgctc ggcgctctgg ccgatgtgcg tgtcgtaacc gggccaccca
     1081 tgatcaagga tgaaaatggt gtgctggtgg gttatgtatt cgccgacatt gacgatacgc
     1141 agcgcgatct gggtggctgg gttgacgacg cgaagcagct ggtcagccaa cgtttcgctt
     1201 tgccgcccgg atattccctg caatggaccg ggcagtatga gttcatggcc gacatgcagg
     1261 cgcgcatgcg ggtcgtgttg ccgctcacgt tcgtactgat tgtcgtgttg ctgtatttgt
     1321 ccatgcgggg cgtgcagcag accctgctgg ttctgctgag tctgccgttc gccatcgccg
     1381 gcagtatctg gctgctggcc ttgctcgact acaacctgtc caccgcggtg tgggtggggc
     1441 ttatcgcggt gggtggtgtg gccgcgcaga ccggcatcgt ggtggtggtc tatctggatc
     1501 aggcgtttca ggcgcgtgta gaaagcggca acctgcgtca gcccggtgat gtggatgacg
     1561 ccgtgataga aggcgccgcc aaatgtctgc gccccatgct gatgacggtg gcaaccactg
     1621 tgctggggtt gatgccgctg ttgtgggaag cgggcgttgg agcagatctt tccgcacgca
     1681 cggcggctcc ggtggtgggt ggtttgtggg cgtgcatgtt tttgtcactc ctggtattgc
     1741 cagcgaccta taccttctgg cgccgttacc agtttggcag ggggaatttt aatcttaggg
     1801 aaatgccgaa tgaataacgg gatggcatct acgaaaacac ccatcggcct cagctcatca
     1861 gaggcagctg ggttgttggc tagcgttggc cccaacgaaa ttcagcacgc tgccgtggta
     1921 ccgccatggc tattgttggc cgggcagttc aagagccctg tcatctggtt gttgctgggc
     1981 gcctgtgtgg tgtcagctgt gctgggggaa gcaaccaatg cgatcgccat tgggtcgatt
     2041 gtcgtcatca atgcatttgt aggattcttt caggagtatc gcgcagagcg cgcggtaaat
     2101 gccctacgat cgatgaccgc tccacgggca aatgtgctgc gcgatggcat gatgaaggtt
     2161 ataccggcaa tggaagtggt tcccggtgat attctactgg ttgaagccgg ggatgtggtg
     2221 gcagcggatg cgcatttgct tgaagcccat tcactttccg tcaatgaagc cgctttgacc
     2281 ggtgagagtc agccatctga aaaatccatt ctacccgtag ccaccaatgc acccttggca
     2341 gagcgtcacg acagcatctt tcttggcacg gcagtggtga gcggtactgc agttgcaaaa
     2401 atcacggcta caggcatggc taccgaactg ggaaaaatag cccatttgct ggcatcggtg
     2461 caggatgaag cgacgccact gcaacagcgt ctcgcacgag tcagtaaaag tctcctgttt
     2521 atttgccttg gtattgtgag tgcggtggca attctgggtg ttgtgcgcgg cacgcccttg
     2581 ctggaagtgt ttctttcagc ggtctcgctg gccgttgcag ccgtgccgga aggactgccc
     2641 gctgttgtta caattgcact tgcagttggc gtgcagcgca tggcggctcg aaaagtactg
     2701 gtacgccgcc ttcctgcggt agagacattg ggatgcacca cagtcatctg caccgacaag
     2761 accggcaccc tgacaacagg tgtaatggct gtgcgcgaac tgtggggcgc cgaccatgtt
     2821 cgtcttctgg atgttgctgc agcgtgctgc aacggagaac tggcacaaga cgagcgaagt
     2881 ggcgtgggtg atacaacgga agtggctttg ctgatggcgg cagcggcccg tggaattcat
     2941 cgaaaggaca tagagcagaa gcgaccgcgt gtaatggaaa acccctttga ttccgagcgc
     3001 aaacgcatgt cgatcctgcg ctcggatggg gtactttatg tgaaaggtgc cgtcgatttg
     3061 ctgttgccgc tttgtcgggg cgacaattca gcagcgctgg cagccaatgc ggacatgagc
     3121 agtcgtggtc tgcgcgtgct tgccatggcc gtcggtaaag gcagggaaga aaacgagctg
     3181 gagctggtgg gattgacggg tattgccgac ccgccacgca cagaagccat tgcggccatc
     3241 gcctctgccc ggagcgcagg cattcgtaca gtcatgatta ctggtgacca tccgataaca
     3301 gcccaggcga tagcgcttga acttggtctt ctgcagaaag gcgaagcggc gcaggagttg
     3361 gtgcatgcgc gagccacgcc ggaagacaag ctgaaaatcg taaaaaaatt caaagaacag
     3421 ggcgctatcg ttgcaatgac gggagacggc gttaatgatg cgcctgcgct gcgagaagcc
     3481 cacattggca ttgccatggg gaaaaacgga accgaggtaa cccgtgaagc agcggatgtt
     3541 gtgctttccg atgacaactt cgccagcatt gtggctgcgg tggaagaagg ccgcggcatt
     3601 ttcgacaata tccgtaaaac actggtttat ctgctggccg gaaacgcagg cgaactcatg
     3661 ctgatgtttt ccgctgcact gctgggtttg cccttgccct tgctgccgct acatttgctg
     3721 tggattaacc tggtgacgga tggtttgccc gcgttggcgc ttgtaaccga cccccttgat
     3781 cgcaacgtca tgaagcgccc tccgcgcgat ccccgcgagc ccattttggg gaaaccccaa
     3841 tggcggcgta ttttggtatc gggactcctt caggcgtcgg tagcgttagc ggtgtttgtt
     3901 tgggcactgg aaaacaggga tttgaatgaa gcacggaatc ttgcatttac cacgttggtc
     3961 tttgcagagt tgtttcgtgc attttccgct cgcagcagct tgccgttctg ggaagtggga
     4021 atattcacca acatgcgcct gttcggaatt gtggtggtgt cggttgttgt tcagttaggc
     4081 attcatcaca tacctgcaac gcaagcgctg tttgaaatac atgatctcag tttggaa
//
LOCUS       DASUEG010000670         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1797268, whole genome shotgun sequence.
ACCESSION   DASUEG010000670 DASUEG010000000
VERSION     DASUEG010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797268"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFX11_12635"
     CDS             complement(<1..518)
                     /locus_tag="VFX11_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4939516.1"
                     /translation="MPEQEGVIQYRLDFTAGPAPDYAHFALLNYWRSYLFRLGLIGQD
                     PQRYGGLGYGNISARLQPDSHSFIVSGTQTGHLPLLCRHHYVRVLDFDVMRNYLRATG
                     PVRPSSEALTHASLYAAHAAIQCVMHVHCPLVWRQAQTMGIPSVDERIGYGTPAMAGA
                     VRALAQASAGNGV"
     gene            complement(633..2057)
                     /gene="der"
                     /locus_tag="VFX11_12640"
     CDS             complement(633..2057)
                     /gene="der"
                     /locus_tag="VFX11_12640"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX4939517.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTKTRDAIVADMPGLTRDRQYGD
                     AAWGEHQFIVIDTGGITGDEEGVDIHMRNQSLQAVEEAHAVLFMVDAQVGLTPVDELL
                     AQTLRMRQKPVFLVVNKVDGLNQDVVTADFFRLGMGDTYPIAAAHGRGVSQLMDAVFA
                     ALPAQPAIEDLGEGVQTGIKMAIVGRPNVGKSTLVNRMLGEERVIVFDMPGTTRDSVY
                     IPFERRDKHYTIIDTAGVRKRGKVFESVEKFSVIKTLQAVEDAHVVVMVLDAREGVVD
                     QDMHLLGFVLETGRSLVVVINKWDGMTHDDKQKVKTDIERRLGFVDFAKIHFISALHG
                     TGVGELYASIQKAYESAFKKLSTPFLTKILEDALTDHQPPLVGGRRIKLRYAHMGGSN
                     PPLIVIHGNQTESVPNSYKRYLENTFRKVMDLEGTPIRIEFKTGDNPFAGKRNLLTPR
                     QIYKKERQVTHHKKKEKKKNKKRF"
     gene            complement(2054..>2257)
                     /locus_tag="VFX11_12645"
     CDS             complement(2054..>2257)
                     /locus_tag="VFX11_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939518.1"
                     /translation="LSYQDGSFKARVKVSSRPTRNPNAKLGQAEPKRTEDGLRAEPVV
                     YQDIVYVQGNGGAVAAYQVIEKE"
BASE COUNT          424 a          730 c          649 g          454 t
ORIGIN      
        1 acgccattgc cggctgatgc ctgcgccagc gcgcgcacgg ccccggccat cgccggtgtg
       61 ccgtagccaa tgcgttcgtc gacgctggga atccccatcg tctgcgcctg ccgccagacc
      121 agcggacaat gcacatgcat cacgcactgg attgccgcat gggccgcgta aagactggcg
      181 tgggtcaggg cttcagagga aggacgcacc gggcccgtgg cccgcagata attgcgcatg
      241 acgtcaaaat ccagcacacg cacatagtga tggcgacaca gcagcggcag atggccggtc
      301 tgggtgccag agacgatgaa ggaatgggaa tccggttgca ggcgggcgct gatattgccg
      361 tagcccagcc cgccgtaacg ttgcgggtcc tgcccgatca ggccgagccg gaacagatag
      421 ctgcgccagt aattgagcaa ggcaaaatgg gcgtaatccg gtgccgggcc ggccgtaaaa
      481 tccagccgat actgaatgac gccttcctgc tccggcatga aaactgcctc ctctggcacg
      541 ccgactccct cacacgcggc cgacgatcct gatccccgtc accgcagccc gccccagggc
      601 aaaccgccat actcccgatc atctgcggaa actcaaaacc gcttcttgtt cttctttttt
      661 tcttttttct tgtggtgcgt cacctgacgc tctttcttgt agatctggcg cggcgtcagc
      721 agattacgct tgcctgcaaa aggattgtcg ccggtcttga attcgatccg gatcggcgtg
      781 ccttccagat ccatcacctt gcggaacgtg ttttccagat agcgcttgta ggaattgggc
      841 acactctcgg tctggttgcc gtgaatcacg atcagcggcg gattgctgcc acccatgtgc
      901 gcataacgca gcttgatgcg gcgaccgccc accagcggcg gctgatgatc cgtcagcgca
      961 tcttccagga tcttggtaag gaaaggtgtc gacagtttct tgaacgcgga ttcgtacgct
     1021 ttctggatcg aggcatacag ctcgcccaca ccagtaccgt gcagggcgga aatgaaatgg
     1081 atctttgcga aatcgacaaa gcccagacgg cgttcgatat cggttttgac tttctgcttg
     1141 tcgtcgtggg tcatgccgtc ccacttgttg atcaccacca ccagcgaacg cccggtttcc
     1201 agcacgaaac ccagcaggtg catgtcctgg tccaccacac cttcgcgggc gtccagcacc
     1261 atcaccacca cgtgggcgtc ttccaccgcc tgcagggttt tgatgacgga gaatttctcc
     1321 accgactcga acaccttgcc gcgtttgcgc acgccggcgg tgtcgatgat ggtgtaatgc
     1381 ttgtcacggc gttcgaacgg tatgtacaca ctgtcgcggg tagtgccagg catgtcgaac
     1441 acgatgacgc gttcttcacc cagcatgcgg ttcaccaagg tggacttgcc gacgttgggg
     1501 cggccgacga tggccatctt gatgccggtc tgaacgcctt cacccagatc ctcgatggca
     1561 ggctgggccg gcagcgcggc aaacaccgca tccatcagct ggctcacgcc acggccatgc
     1621 gccgccgcta tcggataggt atcgcccatg cccaggcgga aaaagtccgc cgtcaccacg
     1681 tcctggttga ggccatccac cttgttcacc accaggaaca ccggcttctg ccgcatgcgc
     1741 agggtctgcg ccagcaactc gtccaccggc gtcaggccca cctgcgcgtc caccatgaac
     1801 agcacggcgt gggcttcctc caccgcctgc agcgactggt tgcgcatgtg gatgtcgacg
     1861 ccttcctcat cgccggtgat accaccggta tcgatcacga tgaactggtg ttcgccccag
     1921 gccgcatcgc catactggcg gtcgcgggtg agccccggca tgtccgcgac aatcgcatcg
     1981 cgggtcttgg tcagccggtt gaacagcgtc gatttgccca cgttggggcg gcccaccagg
     2041 gcgattacgg gtttcattct ttctcgatca cctggtacgc ggccaccgcg ccaccgttac
     2101 cctgcacata cacgatatcc tgatacacca caggctccgc ccgcaagccg tcttcggtgc
     2161 gcttgggttc ggcctggccc agcttggcat tgggattgcg ggtgggccgg gaggacacct
     2221 tcacccgggc cttgaaactg ccatcctggt aggacaa
//
LOCUS       DASUEG010000671         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_258070, whole genome shotgun sequence.
ACCESSION   DASUEG010000671 DASUEG010000000
VERSION     DASUEG010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258070"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFX11_12650"
     CDS             <1..907
                     /locus_tag="VFX11_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939519.1"
                     /translation="KPGNVEWMLCGLLVLLALVGCKQALERTPLASPAFDAGTLEHLR
                     QTHPLNIEQILTEKALLASSGEFRLRNPYVPVTPLSLQACGQDAHCSYSLLRTGLPRL
                     SFPAQPLVYDWWWQRQLQPDQAQVCGVQFADAARRDYRLASFSHHDELTANGFELTHY
                     QACGACSTLQDLAVYGELDLTVMAKVCSKRVSLAEKKSCMQQIGFSEPCAESWAYNAQ
                     KTAQSCALVCVREYGLMALLTGTENVPPVNEKGELNACLLCDEMMSGPGFQYSAGRTR
                     RNSGIISEIDRPDEEVYEVPHRYFE"
     gene            964..>2356
                     /locus_tag="VFX11_12655"
     CDS             964..>2356
                     /locus_tag="VFX11_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX4939520.1"
                     /translation="MLFSNRFLPVCSLILAAIFCTSAAAQTISIVGKHFVNEDGAKII
                     FRGYNIQSKALPFQPIQSETELDILQDMGANLIRLNFVWEAAEPEPGFYNPDYFAYYD
                     RVVKWAWQRGMHVLIDFHNNAFSRYAAKGCGSGFPRWALSPDVAPVEPRTDGSCIFNS
                     AMLQAMLSADNYTHWCDFMTDRHGVRTRFFELTRVLAEKYADHPAVIGFDLNEPMVFQ
                     PILQYDSDLANVFFNDWQQFIMAIDPRYITFFGDSPFQFIFVNQPPHLDIPPTGNVSF
                     DAHFYEAGASSLGRPLFGTATSINAIVATREKYGIPVLVGEFGVNLKGRHNRDFQYQM
                     DQVLRHFDREQLSSTRWNYTPHWNPVQKDHFHDEDFSCFDDQDQVRESCAPRASLQQL
                     SGDLISIDIHHKGEAKLFIPLVPRLSDRLQYSDTRADIRWQHRPEQGATRIFAARQRV
                     FGGGTVTIETEGDD"
BASE COUNT          482 a          681 c          683 g          510 t
ORIGIN      
        1 aaagccgggc aatgtggaat ggatgctgtg cgggctgctg gtgctgttgg cactggtcgg
       61 ctgcaagcag gcactggaaa ggacgccgct ggcgtcaccc gcctttgatg cgggaacgct
      121 ggagcacctg cggcagaccc atccgttgaa tatcgaacag attctgacgg aaaaagcgct
      181 gctggcatcg tcgggcgaat tcagattgcg gaatccctat gtgccggtca cgcccctgtc
      241 gttgcaggcg tgcggtcagg atgcgcattg cagttattcc ctgctgcgaa cggggctgcc
      301 gcggttgagt tttccagcgc agccgctggt gtacgactgg tggtggcaac gccagttgca
      361 gccggatcag gcgcaggtgt gcggtgtgca gtttgccgat gctgcccgac gggattaccg
      421 gctggcgagc ttcagccatc atgacgaact gactgccaat ggttttgagt tgactcatta
      481 tcaggcctgt ggcgcctgtt cgaccttgca ggatctggcg gtgtacggcg aactggatct
      541 gacagtcatg gcaaaagtct gcagtaaacg cgtgagcctc gcggagaaaa aatcctgcat
      601 gcagcagatc ggtttcagcg agccctgcgc cgagtcctgg gcctacaacg cgcagaaaac
      661 ggcgcagtcc tgcgcgctgg tgtgcgttcg cgaatatgga ctgatggcat tgctgacggg
      721 aacggaaaat gtgccaccgg tgaacgaaaa gggcgaactc aatgcctgtc tgctgtgtga
      781 cgagatgatg tcgggccccg gctttcagta cagtgccggc cgcacccggc gcaattccgg
      841 catcatttcg gagatcgacc gtccggacga agaagtgtac gaggtaccgc accggtattt
      901 tgagtaagtg attttcacca tcaggttcac atccaaccag cacaataaca aaggagtgaa
      961 tacatgctgt tttcgaaccg gtttctgcct gtttgcagtt tgatactggc cgcaattttt
     1021 tgtacgtccg ccgccgcgca gaccatttcc attgtgggca agcattttgt caacgaagac
     1081 ggcgccaaga tcatcttccg tggctacaac atccagtcga aggcgctgcc gttccagccc
     1141 atccagtccg aaaccgaact ggatattctg caggacatgg gcgccaacct gatccgcctg
     1201 aattttgtct gggaagccgc agaacccgag cctggctttt acaatccaga ctatttcgcc
     1261 tactacgacc gcgtggtgaa gtgggcctgg cagcgcggca tgcatgtgtt gatcgatttt
     1321 cacaacaacg cattttcccg ctatgccgcc aaaggctgtg gcagcggttt cccgcgctgg
     1381 gcgctgtcac cggacgttgc gccggtcgag ccccgcaccg acggcagctg catcttcaac
     1441 tccgccatgc tgcaggccat gctgagcgcc gacaattaca cccactggtg cgatttcatg
     1501 accgatcgcc atggcgtgcg cacgcggttc ttcgaactca cccgcgtcct ggcagaaaaa
     1561 tatgccgacc atcccgccgt gatcgggttc gatcttaacg aacccatggt gttccagccc
     1621 atcctgcaat atgactccga tctggccaac gtattcttca acgactggca gcagttcatc
     1681 atggcgatcg atccgcgcta tatcaccttt ttcggcgaca gcccgttcca gtttattttc
     1741 gtcaaccagc ccccgcatct ggatattccg cccactggca acgtctcctt cgatgctcat
     1801 ttttatgaag ccggtgccag cagtctgggg cgcccgctgt tcggcactgc caccagtatt
     1861 aacgccattg tcgccacccg cgaaaaatac ggtattcccg tgctggtggg tgagttcggc
     1921 gtcaatctca agggccggca caaccgcgat ttccagtacc agatggacca ggtgttgcgc
     1981 cacttcgacc gcgagcagct ttcctcaacg agatggaatt acacaccgca ctggaatcct
     2041 gtgcagaaag atcatttcca cgatgaggac ttcagttgct tcgacgatca ggatcaggtg
     2101 cgggaaagtt gcgcgccgcg cgccagtctg cagcagttga gcggcgacct gatcagcatt
     2161 gatattcacc acaagggcga ggcgaagctg ttcattccgc tggtgccgcg cctgagcgac
     2221 cgactgcagt attccgatac ccgcgcggac attcgctggc agcatcggcc ggagcagggg
     2281 gcgacccgca tttttgctgc ccgtcagcgg gtattcggtg gtggcactgt gacgatcgaa
     2341 acggagggag acgacc
//
LOCUS       DASUEG010000672         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1095291, whole genome shotgun sequence.
ACCESSION   DASUEG010000672 DASUEG010000000
VERSION     DASUEG010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095291"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VFX11_12660"
     CDS             complement(<1..1133)
                     /locus_tag="VFX11_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939521.1"
                     /translation="MFISQTLRRAVQLNPNGVATIYANRKQSWREFEHRVARLAAGVR
                     SLKVADGDRVAILSLNSDRYLEYFYAIPWAGMALNPINIRLAPPEIAYTLNDSRSSVL
                     FVDDAFSAMLPVLRPLLHNVSHVVFMGEGELPEGCVAYESLIDQHKPLADRSEGGAQL
                     AGLFYTGGTTGRSKGVMLSHDNLVFNALNVVPEMGYGTDSIYMHAAPMFHLADMASTF
                     AVTLAGGSHGIVPRFDVADVLSFMEREKITHTLLVPTMINLLVSSGKIKDFDVSSVKR
                     MLYGASPMPEAVLVSAMEQMPDALFAQGYGQTEASPIITALGPEFHVVGGAKLRSAGR
                     AALGVEVAIMDKDDVELPRGEVGEICARGPNVMLGYWGMEQASA"
     gene            complement(1179..1616)
                     /locus_tag="VFX11_12665"
     CDS             complement(1179..1616)
                     /locus_tag="VFX11_12665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4939522.1"
                     /translation="MSASGTEPLGICCAGLSELQKQVLDDTPYVHFLGLKVSREDDGT
                     TCYQLCFREEHIGNPLLRTFHGGILASFGEIVAALHVVEDPGMQQQPLCTSLTFDYLR
                     PAFAGTIRAVPRIVRAGRRFIVVSVDIFLDKSHVSTGRFIYAR"
     gene            complement(1617..2093)
                     /locus_tag="VFX11_12670"
     CDS             complement(1617..2093)
                     /locus_tag="VFX11_12670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4939523.1"
                     /translation="MTAQSEAANRYLTMAKTFFGTAHPLFNAFGIEIDEIGKGRAVMR
                     MPSNVALSDRNGGLHRGVVVTLLDTNCGLAIFSRLGDMRPIATIDLRVDFIREAPPGE
                     AVRAEVECYAVQGDIAYVKGKAVACSDDSLLASVSGSFAVGTLGPAFDATVTKGAE"
     gene            complement(2164..>2364)
                     /locus_tag="VFX11_12675"
     CDS             complement(2164..>2364)
                     /locus_tag="VFX11_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HEX4939524.1"
                     /translation="GEMLPAVRAAKLVEMMQRSAVAWTGLAALLKMASESDVFPVREI
                     EGAIRHVRTLESQYVHQVTSKV"
BASE COUNT          597 a          743 c          558 g          466 t
ORIGIN      
        1 gcagaggcct gctccattcc ccagtagccc agcatcacgt tcgggcctct ggcgcatatc
       61 tcaccgactt caccacgcgg tagctcgaca tcatccttgt ccataatggc aacctcaacc
      121 cccagcgctg ctctgccagc gctgcgtagc ttggccccgc ccacaacatg aaactccggc
      181 cccagcgcag taataatcgg cgacgcctcc gtctgcccgt acccctgggc aaacagagca
      241 tccggcattt gctccatggc actgaccaac accgcttcgg gcattggcga tgccccgtac
      301 agcattcgct tgacactgct gacatcaaaa tccttgatct tgccggaaga gaccagcagg
      361 ttgatcatgg ttggcacgag caaagtgtgg gtaatttttt cacgctccat gaacgacagc
      421 acatcggcca catcgaaacg cggcacaata ccgtgagaac caccagccag ggtcacggca
      481 aaagtactcg ccatgtcggc cagatgaaac atcggcgctg catgcatata gatggagtca
      541 gttccgtagc ccatttccgg aacaacattc agggcattga acacaagatt atcgtggctc
      601 agcatcacgc cctttgatcg tccggtcgtg ccgcctgtat agaacagtcc ggcaagctgt
      661 gcgccacctt cgctcctgtc agccaggggt ttgtgctggt cgatcagtga ctcataggcg
      721 acacacccct ccggcaactc accttccccc atgaacacca catgactgac gttatgcagc
      781 aacgggcgca gcaccgggag catggcactg aatgcgtcat ccacgaacag caccgatgac
      841 cgggagtcat tcagcgtata agcaatctcc ggaggtgcca gccggatatt aatgggatta
      901 agcgccatcc cggcccacgg gatggcatag aaatattcca gatagcgatc gctattgagt
      961 gaaagaattg ccacacgatc cccgtcagca accttcaatg atcgaacgcc ggcagccaga
     1021 cgagccaccc ggtgctcaaa ttcacgccag ctttgtttcc tgttcgcgta gattgtggcc
     1081 acaccgttcg ggttcaactg gaccgcccga cgcagtgtct gggaaataaa catatcaacc
     1141 tccttggtaa cctgaagatg tcaatacatc aaacgtcctc agcgtgcgta gataaaacgc
     1201 cccgtcgaga catggctctt gtccagaaag atatcgacag aaacgacaat aaagcgccgg
     1261 cctgcacgca cgatccgcgg cacagcacga attgttccgg caaaggccgg ccgcaggtaa
     1321 tcaaacgtca agctggtaca caatggctgc tgctgcatcc cgggatcttc caccacatgc
     1381 aaggcggcga caatttcacc aaaactggca agaatgccgc catgaaaagt acgcaaaagc
     1441 ggattgccga tatgctcttc gcggaaacac aactgatagc aggtcgtgcc gtcatcttcg
     1501 cgactgactt tcagaccaag gaaatgcaca tagggcgtgt catcaagaac ttgtttctgc
     1561 agttcactca acccggcaca gcagattccc agtggctcgg ttccggatgc gctcacttat
     1621 tcagctcctt tcgtcacagt cgcatcaaac gccggaccga gtgtcccgac agcgaaagat
     1681 cccgatacgg aagccaacag cgaatcatct gaacaagcaa ccgcctttcc ctttacatag
     1741 gcgatatcac cctgtaccgc gtaacattcc acttcggcgc gtacagcctc acctggcggg
     1801 gcttccctga taaaatccac acgcaggtca atcgtcgcaa tcggtcgcat atctccaagg
     1861 cgagaaaata tggcgagacc acaattggta tcgagcaagg tcaccacaac accacgatgc
     1921 agcccgccgt tacgatcaga tagcgccacg ttagatggca tcctcataac ggcgcgaccc
     1981 ttgccaatct cgtcaatttc aattccgaac gcgttgaata acggatgagc ggtaccgaag
     2041 aacgtttttg ccatggtcaa gtagcggttt gcggcctcag actgggctgt catctccccc
     2101 cctcattaat acaaaaacca atagcccgaa ccaccaatct ggcggccaac tgaagcatgc
     2161 acgctaaact ttacttgtta cctgatgaac atactgcgac tccagcgtcc tgacatgacg
     2221 aatagccccc tcaatttcac gaaccgggaa cacatccgac tccgacgcca tcttgagtaa
     2281 cgctgccagg ccggtccagg ccaccgctga acgctgcatc atctctacca gtttcgctgc
     2341 ccgcacggcg ggcagcatct ctcc
//
LOCUS       DASUEG010000673         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_670682, whole genome shotgun sequence.
ACCESSION   DASUEG010000673 DASUEG010000000
VERSION     DASUEG010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670682"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1011)
                     /locus_tag="VFX11_12680"
     CDS             complement(145..1011)
                     /locus_tag="VFX11_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEX4939525.1"
                     /translation="MKIPKRLQPLVDDGLVDEVLHSLKSGKEADVFVVRCGEEIRCAK
                     VYKEATKRNFRNNAQYQEGRNVRGSRQARAMEKGSRYGRKEAEEAWLNAEVDALYRLA
                     AAGVTVPQPFGYFEGVLLMELVTDEDGNAAPRLGDVEFSAETARELHGVMISEIVLML
                     CAGLVHGDLSEYNVLIGPDGPVIIDLPQAVFAAGNNNAFSMLARDVQNMSNYFGQFAP
                     ELIGTDYAEEIWDLYKRGELEDATELTGVVTRSEEKIDIDNLMRIIEDARREEEARQR
                     RAREAESGESGE"
     gene            complement(1130..1969)
                     /locus_tag="VFX11_12685"
     CDS             complement(1130..1969)
                     /locus_tag="VFX11_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4939526.1"
                     /translation="MLAAILGLIAGLVLGLTGAGGSVLAVPLLMWGMGWTLPQAAPVA
                     LLAVSASATLGTYTAWRQKIVRYRAALVMAATSMITAPLGLKAAAALPRDLLTGLFAG
                     VLVLIAARLWQQTRTQPEETRVVRAALQGATINSKAPLCELNPDTGRLRWTRPCTLAI
                     TGSGASTGFLAGLLGVGGGFVIVPALRLFTNLPFHSAVATSLMAIALTSAGTAAISIA
                     SGHDLPWLIALPFMAGALSGMLAGRILAPRVAGPRLQQGFALLMLVVAAGMGLKAAGL
                     LPF"
     gene            complement(1973..>2431)
                     /locus_tag="VFX11_12690"
     CDS             complement(1973..>2431)
                     /locus_tag="VFX11_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEX4939527.1"
                     /translation="FKDTPLRLPNLCNPNPDLFCGGMPSPDDLRGAKKAGIRTVVNLC
                     QPQETPPYEASLAQELGLQYVQIPVGGPPDLTEAKASELAAVVDNCANHPVLIHCMSG
                     NRVGALLALKASFADGASAQEALQFGRASGLKALEPAVWQIVSSRTATAG"
BASE COUNT          425 a          760 c          762 g          484 t
ORIGIN      
        1 tcatctccac cggtccggac cgcgtggaaa ccatcatcct gcgcaatccg ttctgatttc
       61 gttttgatga aaacagaaag gggctctcca acctggagtg cccctttttt tgttgcggcc
      121 caaatcctat tgcagtggag cgggttactc gccggattcg cctgattcgg cttcgcgggc
      181 gcggcgctgt ctcgcttctt cctcgcgacg ggcgtcttca atgatgcgca tcaggttgtc
      241 tatgtcgatt ttttcctcgc tgcgcgttac gacacccgtg agctcggtgg catcctccag
      301 ttcgccgcgt ttatacagat cccagatttc ttccgcatag tccgtaccga tcagctccgg
      361 cgcgaactgg ccgaagtaat tcgacatgtt ctggacatcg cgtgccagca tgctgaaggc
      421 attgttgttg ccggccgcaa aaaccgcctg gggcaggtcg atgatcacgg ggccgtcagg
      481 gccgatcagc acgttgtatt ctgacaggtc accatgcacc aggcctgcac acagcatcag
      541 cacgatttcg ctgatcatca cgccatgcag ttcgcgcgcg gtctcggcgc tgaattcgac
      601 gtcgcccagt cgcggcgcgg cgttgccgtc ttcatcggtg accagttcca tcagcagcac
      661 gccttcaaag taaccgaacg gctgcggcac cgtgacgccg gcggcggcaa ggcggtacag
      721 ggcatcgact tccgcattca gccaggcttc ctcggcctct ttgcggccgt aacgcgagcc
      781 cttttccatc gcccgtgcct ggcggctgcc gcgcacgttg cggccttcct ggtactgggc
      841 gttattgcgg aaattgcgct tggttgcttc cttgtagacc ttggcgcagc ggatctcttc
      901 gccgcagcgc acgacgaaca cgtcggcctc tttgccgctc ttgagtgagt gcagcacttc
      961 atctaccagg ccatcatcga ccaggggctg cagtcgtttg ggaattttca tgtaatacgg
     1021 ggcatcctac gtagctgtat ccgatctcat ggggatcatt atatggggtt gagcgcgttg
     1081 agttgaatga aaggtggtgc aggtgcggtt tcaggcgaag caacgacgct cagaatggca
     1141 gtaaccccgc cgctttcaaa cccattcctg ctgccaccac cagcatcagc agtgcaaaac
     1201 cctgttgcag tcgcggtcca gccaccctgg gagccaggat gcgtcccgcc agcatgccgg
     1261 aaagggcgcc ggccatgaag ggcagggcga tgagccaggg cagatcgtgg ccgctggcga
     1321 tggatatcgc tgccgtgcct gcgctggtaa gcgcgatggc catgagcgag gtggcaacgg
     1381 cggaatggaa tggcaggttg gtgaacagtc gcagggcggg aacgataacg aagccgccac
     1441 ccacccccag cagtcccgcc agaaagccgg tgctggcgcc gctgccggtg atggccaggg
     1501 tgcaggggcg ggtccagcgc aggcggccgg tgtccgggtt gagttcgcac agcggcgcct
     1561 tgctattgat ggtggcaccc tgcagagcgg cacggacgac acgggtttct tctggctgag
     1621 tccgcgtttg ctgccacagg cgtgcggcaa tcagtaccag tacaccggcg aacagcccgg
     1681 tcagcaagtc ccgcggcagt gcagcggcgg ccttcaaccc cagcggtgcc gtgatcatgc
     1741 tggtggcggc catcaccaat gcggcacggt agcgcacgat tttctggcgc caggcggtat
     1801 aggtgcccag ggtggcggac gcgcttactg ccaatagtgc caccggcgct gcctgcggca
     1861 acgtccagcc catgccccac atcagcaacg gcaccgccag taccgagcca ccggcgccgg
     1921 tgagccccag caccaggccg gcaatcaggc cgaggatcgc cgccagcacg gttcagcctg
     1981 ctgtcgctgt gcggctggat acaatctgcc acaccgctgg ctccagcgcc ttcaatccgc
     2041 tggcgcggcc gaactgcagg gcttcctgag ccgaggcacc gtccgcaaag ctggctttca
     2101 gcgccagcag ggcgcctacc cggttgccgc tcatgcagtg aatcagcacc ggatggttgg
     2161 cacagttgtc taccacggct gccagttcgc tggccttggc ttcagtcagg tcgggtggac
     2221 cgcccaccgg aatctgcaca tattgcaggc ccagttcctg cgccaggctg gcttcgtagg
     2281 gaggtgtctc ctgcggctgg cacaggttaa ccacggtgcg gattccggcc ttctttgcgc
     2341 cgcgcagatc gtcggggctg ggcattccgc cgcagaacag gtcgggattg gggttgcaca
     2401 gattgggcag gcgcaggggc gtatctttaa a
//
LOCUS       DASUEG010000674         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1421993, whole genome shotgun sequence.
ACCESSION   DASUEG010000674 DASUEG010000000
VERSION     DASUEG010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421993"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFX11_12695"
     CDS             complement(<1..203)
                     /locus_tag="VFX11_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX4939528.1"
                     /translation="MLELDVAQLYQWIGQYFFPLCRISAFFMVLPIFSTQLVPMRIRM
                     GLAVATTVVIAPVIAPTPAQELLS"
     gene            complement(207..476)
                     /gene="fliQ"
                     /locus_tag="VFX11_12700"
     CDS             complement(207..476)
                     /gene="fliQ"
                     /locus_tag="VFX11_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX4939529.1"
                     /translation="MTPEVISDLMREALYLSMIMVMVIIVPSLCVGLVVSVFQAATQI
                     NEQTLSFLPRLLVTLLTLIVLGPWLGGMVTDFTERLIHDLPTLLG"
     gene            complement(492..1247)
                     /gene="fliP"
                     /locus_tag="VFX11_12705"
     CDS             complement(492..1247)
                     /gene="fliP"
                     /locus_tag="VFX11_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018983419.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX4939530.1"
                     /translation="MKNNGLWLAFMGLLMLSGPVLAADPMSLPAVNLATNPDGTQTYS
                     ITLQVLVLMTALTLLPAILMMMTAFTRIIIVFSILRQAIGLQSTPSNQILIGLAIFLT
                     IFVMSPVLKEINEKALQPYMKEELQPLAAVEVASVPLRKFMLAQTREADLNLFVELAG
                     QDRKFASLDEVPLTIVIPAFVTSELKTAFQIGFLLFIPFLIIDLVVASVLMAMGMMML
                     SPMIVSMPFKIMLFVFVDGWAMVVGTVASSFGL"
     gene            complement(1237..1725)
                     /gene="fliO"
                     /locus_tag="VFX11_12710"
     CDS             complement(1237..1725)
                     /gene="fliO"
                     /locus_tag="VFX11_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX4939531.1"
                     /translation="MKWRLALVLLLGLCVSPVRADAPAAQVDAAAATAPSPAVSELRL
                     KTEPVRGDSKSMGAASAQMVLGLAAVLAVIFALAWLARRFNLSGVGVTTGMRVLGALA
                     VGPKEKILMVEVEGKRLLLGVTAHQISLLQTWDSDAETPDSADFAGKIQALLKTGPLH
                     EK"
     gene            complement(1732..2145)
                     /gene="fliN"
                     /locus_tag="VFX11_12715"
     CDS             complement(1732..2145)
                     /gene="fliN"
                     /locus_tag="VFX11_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX4939532.1"
                     /translation="MAGSDKPVDDQALADEWAAALAEQETSDAEPGVRAAPLESLRDD
                     AMPMSGDGPNLDVILDIPVTLSMEVGSTNISIRNLLQLNQGSVIELDRLAGEPLDVLV
                     NGTLIAHGEVVVVNEKFGIRLTDVISPTERIKKLG"
     gene            complement(2159..>2690)
                     /locus_tag="VFX11_12720"
     CDS             complement(2159..>2690)
                     /locus_tag="VFX11_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX4939533.1"
                     /translation="ILPVKFEYISSEVNPSLANIVSPSEVVVVNSFHVELDGGGGEMH
                     LTLPYSMIEPLREMLDAGVQSDVGDQDERWMKSLREEIMEAQINLKGKLLEKKIRLSE
                     LSRLKPGDIIPIEMPETLMVLANGIPTFRGKMGEANGNLAVKVTSKVRRPESDVGKQS
                     RLSQLKEQMKSEESKF"
BASE COUNT          599 a          872 c          685 g          534 t
ORIGIN      
        1 gacagcaatt cctgcgccgg cgtcggtgcg atcaccggcg cgatgaccac ggtggtcgcc
       61 accgccagcc ccatgcggat tcgcatcgga accagctggg tgctgaaaat cggcaacacc
      121 atgaagaagg cggaaatgcg gcacagcgga aaaaaatact ggccgatcca ctgatacagc
      181 tgagcaacat ccagctccag catggatcac ccgagcaggg tcggcaaatc gtggatcagc
      241 cgttcagtga aatccgtcac catgccaccc agccaggggc ccagcacaat caaggtcagc
      301 agggtaacca gcaggcgcgg cagaaaactc agggtctgtt cgttgatctg tgtcgccgcc
      361 tgaaacactg acaccaccag ccccacgcac aggctgggca caatgatcac cattaccatg
      421 atcatcgaca ggtaaagcgc ttcccgcatc agatcggaga taacctccgg cgtcatgaca
      481 aagctcctcc gctagaggcc aaagctggag gccaccgtgc ctaccaccat ggcccagcca
      541 tccacaaata cgaacagcat gattttgaac ggcatggaaa cgatcatggg cgacagcatc
      601 atcataccca tcgccatcag cacgctggcc accaccagat caatgatgag aaacggaatg
      661 aacagcagaa acccgatctg gaacgccgtt ttcagttcgc tggtgacgaa agccggaatc
      721 acgatggtca gcggcacttc atccagcgat gcgaatttgc gatcctggcc cgccagttcc
      781 acaaacaggt tgagatcggc ttcgcgggtt tgcgccagca tgaacttgcg caggggaacg
      841 ctggccacct ccactgccgc cagtggctgc agctcttctt tcatgtaggg ttgcagtgcc
      901 ttctcgttga tttccttcag cactggcgac atcacaaaga tggtgagaaa aatggccaga
      961 ccaatcagga tctggttcga cggtgtggac tgcaggccaa tcgcctgccg cagaatggaa
     1021 aagacaatga tgatgcgggt gaaggcagtc atcatcatca ggatcgccgg cagcagggtc
     1081 agcgccgtca tcagcaccag tacctgcaag gtgatcgaat aggtctgggt gccgtctgga
     1141 ttggtggcca gattcactgc cggcagcgac atgggatcgg ccgccagcac cgggccgctc
     1201 agcatcaaca ggcccatgaa ggccagccac aatccattat ttttcatgca gtggtcctgt
     1261 cttcagcaat gcttgaatct tgcccgcgaa atccgcgctg tccggcgttt cagcgtccga
     1321 gtcccaggtt tgcagcaagg aaatctgatg tgccgtcacg cccagcagca ggcgttttcc
     1381 ctcgacttcc accatcagga ttttctcctt cggcccaacc gccagggctc ccagcacccg
     1441 catgcccgtg gtcacaccga caccactgag attgaaacgc ctggccagcc aggccagggc
     1501 aaaaatcacc gccagcaccg cagccaatcc cagtaccatc tgcgcgctgg cagctcccat
     1561 gctcttgcta tcaccccgca caggctccgt cttcagccgc agttccgata ccgccggcga
     1621 tggcgcagtg gccgccgccg catccacctg cgctgccggc gcatcagccc gcaccgggct
     1681 cacgcacaat cccaacagca gcacgagtgc aagtcgccat ttcatgtcgg ttcaacccag
     1741 tttcttgatg cgctcggtgg gactgatgac atccgtcaga cggataccaa acttctcgtt
     1801 caccaccacc acttcaccgt gcgcaatcag ggtgccattc accagcacat ccagcggttc
     1861 gcccgccaga cggtccagtt cgatgactga accctgattc agctgcaaca gattgcggat
     1921 gctgatattg gtgctgccca cttccatcga cagcgtgaca ggaatgtcca gaatcacatc
     1981 cagattggga ccgtcgccgg acatgggcat ggcatcatcg cgcaggcttt ccagcggcgc
     2041 cgcacgcaca cccggctcgg catctgacgt ttcctgttct gccagtgccg ccgcccactc
     2101 atcggccaga gcctggtcat ccacgggttt atctgaacct gccatatctc actccaccct
     2161 aaaacttcga ttcttcgctc ttcatctgtt ctttcagctg tgacaaccgc gactgtttgc
     2221 cgacatcaga ctccggtcgc cgcaccttgc tggtgacctt cactgccagg ttgccattgg
     2281 cctcgcccat ttttccgcgg aacgtgggga tgccattggc cagcaccatc agcgtttccg
     2341 gcatttcgat cggaataatg tcacccggct tcagtcgcga cagttccgac agtcgaattt
     2401 tcttttccag cagcttgccc ttgagattga tctgcgcttc catgatttcc tcccgcaggg
     2461 atttcatcca gcgctcgtcc tggtcaccca cgtcgctctg cacgccggcg tccagcattt
     2521 cccgcaacgg ctcgatcatg gaatagggca atgtgagatg catttcgcca ccaccgccat
     2581 ccagctcgac atggaatgaa ttcaccacca ccacttcgct cggactgacg atgttggcca
     2641 gtgacggatt cacttcggaa ctgatgtatt caaacttcac cggcagaatc
//
LOCUS       DASUEG010000675         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1005293, whole genome shotgun sequence.
ACCESSION   DASUEG010000675 DASUEG010000000
VERSION     DASUEG010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005293"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFX11_12725"
     CDS             <1..236
                     /locus_tag="VFX11_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEX4939534.1"
                     /translation="TDIIADTQRAVAEANHDGRVAGLINFNCILRTLELKSRQLTQAY
                     ADLFPVPMAGFSTYGEANIGHINQTATMIAFLR"
     gene            427..1719
                     /locus_tag="VFX11_12730"
     CDS             427..1719
                     /locus_tag="VFX11_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX4939535.1"
                     /translation="MNHTANTAPDTGHSRRALMVLLSSTWAFTICFAAWMIFAVLGIP
                     IKQQLGLNETEFGLLAAMPVLTGSLVRVPLGIWTDRCGGRAVQMGLMLSTVIPIWLLS
                     HATEYWHFLVLALFVGVAGGSFSVGTPYVARWFPRERQGLAMGVFGAGNSGSALTKFV
                     APVLIAASGSWLIVPKVYAVAMLITAVLFWIFSASDPAHVVKSGTSLRDQMHLLRDRR
                     VWRYCQYYSVVFGGYVALALWMTRYYISEYGFDMQLAALLAACFSLPGGVLRALGGWI
                     SDRHGAYRTTWWVMWICWVAFFLLSYPQTQFTIHTVNGPQSFFIGLNPLLFTILLFTV
                     GVAMAVGKASVFKFIADDFPTQIGAVSGIVGLAGGLGGFLLPILFGLAVDLTGVRSSC
                     FMLLYGTVCVSLICMHFSFRPERMAADQRALAAQGAAA"
     gene            1759..>2317
                     /locus_tag="VFX11_12735"
     CDS             1759..>2317
                     /locus_tag="VFX11_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4939536.1"
                     /translation="MSSHVLQRWEPESPAFWQTTGSGIAWRNLCISIPALMLAFSVWM
                     LWSVAVVNLDKAGFNLSKNQMFWLTALPALSGATLRIFYSFLVPIFGGRRWTAISTAS
                     LLIPALGMGYALQDPTTSYPTLLVLALLCGLGGGNFSSSMSNISFFFPKSKKGLATGL
                     NAGIGNLGVSLVQFVVPMVIAGSFLG"
BASE COUNT          351 a          725 c          716 g          525 t
ORIGIN      
        1 cgacggacat cattgccgat acgcagcggg cggtggccga ggccaaccac gacgggcggg
       61 tggccggtct gatcaacttc aactgcatcc tgcgcacgct ggagctcaag tcacggcagc
      121 tgacgcaggc ttatgccgat ctgttcccgg taccgatggc aggcttttcc acctacggtg
      181 aggcgaatat cggccatatc aaccagacgg caaccatgat tgccttcctg cgctgacgcg
      241 catcgggcgg cgtatcgggg ggcgttgtcg ccagcagcga caacgccccc tggcttttgt
      301 agcgcgcccc gccatccgga ctagttcctc tgcacccccg cagctcctac ctgtggcgaa
      361 tattgccgcg acggcaggca gcataagctg acaggcatta tcacagcgat cagggggaga
      421 gcagccatga accacactgc caatacggcg cccgacaccg gccatagccg gcgggcgctc
      481 atggtgctgc tgtcgagcac ctgggcgttt accatttgtt tcgccgcctg gatgattttt
      541 gccgtgctcg gcattcccat caaacagcaa ctgggcctga acgaaaccga gtttggcctg
      601 ctggcggcca tgccggtgct gaccggctcg ctggtgcgtg taccgctggg tatctggacc
      661 gaccgctgtg gtggccgcgc ggtgcagatg ggcctgatgc tgagcacagt cattccgatc
      721 tggctgctca gccatgcgac cgagtactgg cactttctgg tgctggccct gtttgtcggt
      781 gtggctggcg gctcgttttc cgtcggcacc ccatatgtgg cccgctggtt tccgcgtgag
      841 cgccagggcc tggccatggg cgtgtttggc gccggcaatt ccggctcggc attgaccaag
      901 tttgtcgccc cggtactgat tgctgccagc ggcagctggc tgattgtgcc caaggtgtac
      961 gcggtggcca tgctcattac cgcggtactg ttctggatct tctcggccag cgatccggcg
     1021 cacgtggtga agagcggcac ctcgctgcgc gatcagatgc acctgctgcg cgaccggcgc
     1081 gtgtggcgct attgccagta ctactcggtg gtatttggcg gctatgtggc gctggcgctg
     1141 tggatgacgc gctactacat cagcgagtac ggttttgaca tgcagctggc cgccttgctg
     1201 gccgcctgct tctcgctgcc gggcggcgtg ctgcgcgccc tgggcggctg gatttccgac
     1261 cggcacggcg cctaccgcac cacctggtgg gttatgtgga tctgctgggt tgccttcttt
     1321 ttgctcagct atccgcagac acagttcacc atccatacgg ttaacggccc gcagagcttc
     1381 tttatcggcc tcaatccgct gctgttcacc attctgctgt ttaccgtcgg ggtggccatg
     1441 gccgtgggca aagcctcggt cttcaaattc atcgctgacg attttcccac ccagatcggt
     1501 gccgtgtccg gcattgtcgg cctcgccggt ggcctgggcg gtttcctgct gcccatcctg
     1561 ttcggtctgg ccgtcgacct gaccggcgtg cgcagcagct gcttcatgct gctgtatggc
     1621 accgtgtgcg tatcactgat ctgcatgcac ttcagcttcc gtcccgaacg catggcggct
     1681 gaccagcggg cactggcagc ccagggcgct gcggcctgag cccggatttt tttaccaccc
     1741 agtacaggga gtaacaccat gtcttctcat gttctgcaac gctgggagcc ggaaagtccg
     1801 gcgttctggc aaaccacagg ctcaggtatt gcctggcgta atctgtgcat ttccattccg
     1861 gcgctgatgc tggccttcag cgtgtggatg ctgtggagcg tggcggtggt caatctggac
     1921 aaggccggct tcaacctgtc gaaaaaccag atgttctggc tgacggcact gcccgccctg
     1981 tccggcgcta cgctgcgcat tttctacagc tttctggtgc ccatcttcgg tggccgccgg
     2041 tggaccgcca tttccaccgc cagcctgctg attccggcgc tgggtatggg ttatgccctg
     2101 caggacccga cgaccagcta cccgaccttg ctggtgctgg ccctgctgtg cggcctgggt
     2161 ggtggcaatt tcagctccag catgtccaat atcagtttct ttttccccaa gtcgaaaaaa
     2221 ggactggcca ccgggctgaa cgccggcatc ggcaatctgg gcgtcagcct ggtgcagttt
     2281 gtcgtgccca tggtcatcgc cggatcgttt ctgggcg
//
LOCUS       DASUEG010000676         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_494010, whole genome shotgun sequence.
ACCESSION   DASUEG010000676 DASUEG010000000
VERSION     DASUEG010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494010"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFX11_12740"
     CDS             complement(<1..441)
                     /locus_tag="VFX11_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939537.1"
                     /translation="MSEQAIKDLAREYTRRTLSQSATFHQLGVDDQKALYLDVYRQNY
                     QQLAQNANGGRSQQQGLQSRAFADPKKASDMIDDTRHTNKRIDQAGELAGGFIDEVDF
                     PGFVKDLLKGVFDANLEVTLAQMESYQKLLKAATQSVSKFINAID"
     gene            complement(466..1680)
                     /locus_tag="VFX11_12745"
     CDS             complement(466..1680)
                     /locus_tag="VFX11_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939538.1"
                     /translation="MPSNIKISLTPGALEAAVLKLINSVNEQGQLPALLEVYIEGSEI
                     DQTRFTPEVKAAMIDYLLDRGIQIEDVEKLKAGGYDEHFALAYDYAVSSASGEDDPLD
                     AARRKGGGRGFGQWDFTVDDFSEVEEQGIVKDNILAAGALDYIFEFGERLGVFNLVDA
                     LVLNWSAGSIDVVEGAAAARLYRYWKLREERASAEERGMVYRRVLNKGNTEVLSRMVV
                     NEPFPQLWHNLMAEVAEYIDKTERVDEGIGEFSPVSRSRIYQATRELQYNLTEYCTGM
                     AHVQAREFYAQLQECFEILKDDEIMAYFGGNRRKSLWTVIERLSKEEFGASPNIAAHR
                     SLAVDGNKIFVWIANFNESSVRQEDFAAFLQAAESYILNFTNASGDEMDFGDEDMLEE
                     EEEFADSESDDF"
     gene            complement(1702..>2019)
                     /locus_tag="VFX11_12750"
     CDS             complement(1702..>2019)
                     /locus_tag="VFX11_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011397686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939539.1"
                     /translation="GWATEVDRVTKRINGLRISENKRVILAQVYPYVLNTLLSCNEAE
                     KEVKDEIGFTEAVLFELERENGPLDKFSQEVVKLVGAKHRINFTEDNVLRDLIAHQVR
                     AGL"
BASE COUNT          405 a          619 c          527 g          468 t
ORIGIN      
        1 gtcgatagca ttgatgaatt tggacaccga ctgcgtggcc gccttcagca gcttctggta
       61 cgactccatc tgtgccaatg tcacttccag gttggcatca aacacgcctt tcaacaggtc
      121 ttttacaaag ccgggaaaat ccacctcgtc gataaagcca ccggccagct cgcccgcctg
      181 atcaatgcgc ttgttggtgt ggcgggtgtc gtcgatcatg tcgctggcct ttttggggtc
      241 tgcgaaagct ctgctctgca agccctgctg ctgcgaacgc ccaccgttcg cgttctgcgc
      301 cagctgctgg tagttctgcc gatacacgtc cagatacagc gctttctgat cgtccacgcc
      361 cagctgatga aaagtggcgg actgggacaa tgttctgcgc gtatattccc ttgccagatc
      421 cttgattgct tgttcgctca ttggattgaa cccctcagcc catcatcaga aatcatcgct
      481 ttcggaatcg gcgaattctt cttcctcttc cagcatatcc tcatcgccga aatccatctc
      541 atcgcccgag gcattggtga aattcaggat gtaggactcc gccgcctgca ggaatgcggc
      601 aaaatcctcc tgccgcaccg acgactcgtt gaagttggcg atccacacga aaatcttgtt
      661 gccatccacc gccagtgaac ggtgtgcggc aatattgggc gaggcaccga attcctcctt
      721 cgacagacgc tcgatcaccg tccacagact cttgcgccgg ttgccgccga agtaggccat
      781 gatctcgtcg tctttcagga tttcaaaaca ttcctgcagc tgggcgtaga actcccgcgc
      841 ctgcacgtgc gccatgcccg tgcaatattc ggtgagattg tattgaagct cacgggtggc
      901 ctggtaaatg cgcgagcggg acaccggcga gaattcacca atgccttcat cgacccgctc
      961 ggtcttgtcg atgtattccg ccacttccgc catcaggtta tgccatagct gcgggaaggg
     1021 ctcgttcact accatgcgcg acagcacctc ggtgttgccc ttgttgagaa cgcgacgata
     1081 gaccatgcca cgttcctcgg cagacgcccg ttcctcgcgc agtttccagt aacggtagag
     1141 acgggcagcg gctgctcctt ctaccacgtc gatggaaccg gcagaccagt tcagcaccag
     1201 cgcatccacc agattgaaca cgcccaggcg ttcgccgaat tcgaagatgt aatccagggc
     1261 gccggcagcc aggatgttgt ccttgacgat gccctgttcc tccacctcgg aaaaatcatc
     1321 gacggtgaaa tcccactggc caaaaccacg gccaccaccc ttgcggcggg cggcatccag
     1381 cggatcatcc tcgccactgg cagaactgac agcgtagtcg taggccagcg cgaagtgctc
     1441 gtcgtaaccg ccggccttca gtttttccac gtcttcgatc tgaatgccgc gatccagcag
     1501 gtagtcgatc attgcggcct tcacttcagg cgtgaagcgg gtttgatcga tttcactgcc
     1561 ttcaatgtag acttccagca gcgccggcaa ctggccctgc tcattgacgg agttgatgag
     1621 tttgagaacg gccgcctcaa gggcgcctgg tgtcagcgag attttgatat tgcttggcat
     1681 ggtcggcatc ccgttcgcaa attaaagacc ggcacgaacc tgatgggcaa tcaggtcgcg
     1741 cagcacattg tcttcagtga aattgatgcg gtgcttggca cccaccagct tcaccacttc
     1801 ctgcgagaac ttgtccaggg gaccgttttc ccgctccagt tcaaacagca cggcttcggt
     1861 gaagccaatc tcatccttca cttccttttc tgcctcgttg cagctgagca gtgtgttcag
     1921 cacataggga taaacctgcg ccagaatcac gcgcttgttc tcgctgatgc gcaggccgtt
     1981 gatgcgcttg gtgacgcggt ccacttcggt ggcccagcc
//
LOCUS       DASUEG010000677         4780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1382511, whole genome shotgun sequence.
ACCESSION   DASUEG010000677 DASUEG010000000
VERSION     DASUEG010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="VFX11_12755"
     CDS             <1..2355
                     /locus_tag="VFX11_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX4939540.1"
                     /translation="DLPTRFTDEAGRDRNYGYDGFGRLVSATDFAGQAAAYVWGGSGL
                     ESVTDAEGSGFTFAYNSFGRVSNLSFSDGSGKGLTYDSRRNLSSVTLTSGASKTLTYD
                     SMGRLLTDTRSTGELFEFSWDPNGRLLGAADALGTASYRYDPTGNVAEYVNSAGDSIA
                     YEFDAVGRVTGQTITNDAGNQQRQNGYEYDGAGRLVKVLDASGGETIMAYDAAGRLST
                     RELPNGVTTAYTYNDRDFVTGIVHSVSGNVISSVTYLRNVGGEPSRITWQDGSYVLLG
                     YDSALRVIDERFHDVANNLVRHIQYEYDRVGNRTAKVVDGSRTAFAYGAGHRLLSASG
                     AETHLYGYDVDGRVTRVEQPALTADLGYNFAGQIAHASVAGVNVSYTYDAAGRRIQAN
                     RNGTVRRYLRTEAVTGAYENPQAILDADSNLLQTYTYAGDTPLMRSDGTQTRYYLTDA
                     QGSVIAVTDESGNVVGTVKYDAYGVELETTGQMVLPADLGGDFRFQGQWKDAVTGLYN
                     MRARDYDPVSGRFLSADPAEPDYKEPESLNRYLFANANPYLFADPSGRFTLISVNISL
                     NIQATLRSIAVNIAKDYFLDKARSVMGSMVLGALKNFTALESFSPWGFVGVNNPAEAG
                     RVWEQKIQNFICSMVPDTFREIVWFEPAVGGGYASRNGYGCPGGGGTFAGAGSAKPDF
                     ILSKTPPVELGRPPKTSIKAYLIGESKWRLKTFYNDYVLNRGGKRNQLQQIVRFAADR
                     VYSRTAVFLALKDETTAAEKAELSALLIREGVDEGAIPIMVSAQ"
     gene            2493..3302
                     /locus_tag="VFX11_12760"
     CDS             2493..3302
                     /locus_tag="VFX11_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939541.1"
                     /translation="MIHPRQHKAFLEKLYVEQSMPLAEAVRLFSSGKAPSFDEFSQAI
                     NFLWVEDLFSIRKKDSSDEVWLDKAMLKQPDDYVIALKSIAGKDRNIAEGYKSRILDN
                     EVNVFSDKHCWASLKPKGHLKIVGDRDGATNLAERIRAFTQYFACTLLARDFSRVSGF
                     VSAAVAEQYSAEMLEQTMAHHESDYGRFEFFDHVRVEGVYSGDQADVQINDQMKLPKG
                     IATSQRRGEASFKLVSACSPNGIAIHSVSVVLAIVEENERLKVCDIRWQRN"
     gene            3478..3666
                     /locus_tag="VFX11_12765"
     CDS             3478..3666
                     /locus_tag="VFX11_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939542.1"
                     /translation="MAAVAGRELQRVPAGWDGAEPGEQYVYVENPVALRVVGDLDGAA
                     ALSCFILPACTGRMVWRW"
     gene            complement(3797..4546)
                     /locus_tag="VFX11_12770"
     CDS             complement(3797..4546)
                     /locus_tag="VFX11_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX4939543.1"
                     /translation="MSGSDLDIILPALVAGILVLFTHVPLGREVVRRGIIFIDLAIAQ
                     IAGVGVIAARMLDFESAWAVQLFASAAALGGALLLAFTDRKMGTLQEPLIGVLFVLAA
                     TGGLLLLAQDPHGGDALKEMLAGQILWVDPTQLIWSAGVLVPCGLLWLALGRHLGNAG
                     FYVLFALVITTSVQLVGVYLVFASLVVPALAVRRWEGRGGVMAGWALGACGYLAGLYC
                     SLYWDLPAAPMIVWGLAGLALIFSWGTGRDG"
     gene            complement(4543..>4780)
                     /locus_tag="VFX11_12775"
     CDS             complement(4543..>4780)
                     /locus_tag="VFX11_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX4939544.1"
                     /translation="GIPPSGADQKRLLETLTADKPAAILVAAFENAKAAHWLGEKMQV
                     PVIVLPFTVGGDEQSADLPTLFTRTLQLLNGSAP"
BASE COUNT         1014 a         1328 c         1425 g         1013 t
ORIGIN      
        1 gacctgccca cccgattcac cgatgaagcc ggtcgcgacc gcaattacgg ttacgacgga
       61 ttcggccgcc tggtatcggc aacggatttt gccggacagg ctgccgccta tgtatggggt
      121 ggcagtggcc tggaatcggt gacggacgcg gaaggatccg gtttcacttt tgcctacaac
      181 tcgtttggcc gcgtatcgaa cctcagtttt tctgacggtt cgggcaaagg gttgacctat
      241 gattcccgcc gtaacctgtc cagcgtcacg ctgacatccg gtgcgagcaa aacgttgacg
      301 tatgattcca tgggccgttt gctgacggat acccgctcca cgggtgaact gtttgaattc
      361 agctgggatc cgaacggacg cttgctgggt gctgctgatg ccttgggaac tgcatcctat
      421 cgctatgacc ccaccggtaa cgttgcggaa tatgtcaaca gcgcgggaga ttccatcgcc
      481 tatgagttcg atgccgtcgg tcgcgtcacc ggtcagacca tcaccaacga cgcgggcaac
      541 cagcagcggc agaatggtta tgaatatgat ggcgcgggac gtctggtcaa ggtgctagat
      601 gccagcggtg gtgaaaccat catggcctat gatgcggcgg gccgcctgag cacccgcgag
      661 ttgcccaacg gggtgaccac ggcttatacc tacaatgatc gtgacttcgt aaccggcatc
      721 gtgcactcgg tatcgggcaa tgtgatcagt tcagtgacct atctgcgcaa tgtgggtgga
      781 gagcccagcc gcatcacctg gcaggatggt agctatgtcc tgctgggata tgacagtgcg
      841 ttgcgggtca tcgatgaacg gttccatgac gttgccaaca atctggtccg ccacattcag
      901 tatgaatacg atcgcgttgg caatcgtacc gccaaggtgg tggatggctc ccgcacggcg
      961 tttgcctatg gtgcaggcca ccgtctgctg agcgcgtccg gcgcggaaac gcatctgtat
     1021 ggttacgatg tcgatggcag ggtcacccgg gttgaacagc ccgcccttac agcggatctg
     1081 gggtacaact ttgcaggtca gattgcccat gcttcggtgg ctggcgtcaa tgtcagctat
     1141 acctacgatg cagcgggtcg ccgtattcag gccaatcgca acggcactgt gcggcggtat
     1201 ctgcgcaccg aggctgtgac cggcgcctac gaaaatccgc aggcgattct ggatgcggac
     1261 tccaatctgc tgcagacata cacctatgcc ggcgatacgc cattgatgcg gtctgacggt
     1321 acgcaaaccc gatactatct gaccgatgcc cagggttccg tcatcgctgt gactgatgaa
     1381 tccggcaacg tagtgggcac agtgaagtat gacgcctatg gtgttgaact ggagaccaca
     1441 ggacaaatgg tattgcctgc tgacctgggt ggcgacttcc gtttccaggg acaatggaag
     1501 gatgcggtga caggactcta caatatgcgg gcacgtgact atgaccccgt ctctggccgc
     1561 ttcctgtcgg cagatccggc cgagccggat tacaaggaac cggaaagctt gaaccgctat
     1621 ctgttcgcga atgccaaccc ttacctgttc gcggatccgt ccggacgctt cacgctgatc
     1681 agtgtcaata tcagtctgaa cattcaggca accctgcgct ccattgccgt caatattgcc
     1741 aaggattatt tcctggacaa ggcgcgtagt gtcatgggtt cgatggtgtt aggtgccctg
     1801 aaaaacttta ccgcactgga atccttcagt ccctggggct tcgtgggcgt caataatccg
     1861 gccgaggcgg ggcgtgtttg ggagcagaag atccagaatt tcatctgttc catggtgcct
     1921 gacacgttcc gtgagatcgt ctggttcgag ccagccgtcg gtggcgggta tgccagccgc
     1981 aatggttatg gatgcccggg gggcggcggt acttttgccg gcgcaggctc ggcgaaaccg
     2041 gatttcattc tgtcgaaaac acctccggtg gagctgggtc ggccgcccaa aaccagcatc
     2101 aaggcgtacc tgattggcga aagcaaatgg cggttgaaaa ccttctataa tgactatgtg
     2161 ttgaatcgcg gaggcaagcg caaccagttg cagcagatag tacgttttgc tgccgacagg
     2221 gtttattcgc gtactgccgt gttcctggca ctcaaggatg agaccaccgc tgcagagaag
     2281 gccgaattga gtgctctgtt gattcgtgaa ggtgttgacg aaggagccat accgatcatg
     2341 gtgagcgccc aatagcgaac ggatttcatc gttgccgcca tcggcggaga gtccgtattc
     2401 atgtcggcac ccgacctctg cagtgcgcag gggttcggtg ccgaattttt atgttttctt
     2461 tctggttgaa tgacaggagt tggagcagcg tcatgattca tccaagacag cacaaagcct
     2521 ttctggaaaa actttacgtg gagcagtcga tgccgctggc ggaggcggtt cggttgtttt
     2581 cttccggcaa ggccccgtcc tttgacgagt tcagtcaggc gatcaatttt ctgtgggtgg
     2641 aagatctgtt ttccatccgg aaaaaagatt cttcagacga agtgtggctg gacaaggcaa
     2701 tgctgaaaca gccggacgac tatgttattg ccctgaaaag catcgcggga aaggaccgta
     2761 atattgccga aggttacaaa agcaggattc tggacaatga agtcaacgtt ttttcggaca
     2821 agcactgttg ggccagtctc aaaccaaaag ggcatctgaa aatagtgggt gaccgggatg
     2881 gtgccactaa tctggcggaa cggattcgtg cattcaccca gtatttcgcc tgcacattgc
     2941 tggcgcggga tttttcgcgg gtgtccggtt tcgtgtccgc cgcggttgcg gagcagtatt
     3001 cggcggaaat gcttgagcag accatggcgc atcacgaaag tgactacggc cggttcgagt
     3061 ttttcgatca tgtaagggtt gagggtgttt actcgggtga tcaggcggac gttcagataa
     3121 atgatcagat gaagttgccg aaaggaattg ccacgtccca gcgccggggt gaggccagtt
     3181 tcaagctggt cagtgcatgc tcgcccaacg gaatcgccat ccacagtgtc agcgttgttc
     3241 tggcaatagt ggaggaaaat gaacggttga aggtgtgcga tatccgttgg cagcgcaatt
     3301 gaaaatttgt cggctacagc cattttgttc cggtattgtc gagtcggagc cgctgcccgc
     3361 cgcgcttcgc aacgagaatg gttgaatgaa actgtccgtt acctatttcc cagacggaaa
     3421 tctggggtta agccgcacca agatgcactg gttctgagca gcccgggtga aatagagttg
     3481 gctgcggttg ctggtcggga actgcaacgg gtgccggctg gctgggacgg tgcggagcct
     3541 ggcgaacagt acgtgtacgt ggaaaatccc gtagcgctgc gcgttgtcgg tgatctggac
     3601 ggcgctgctg ccctgtcgtg ctttatcttg ccagcgtgca caggccgaat ggtgtggcgc
     3661 tggtgacttg cccgctgtat ctggccatca tcgaggaaga cgggatgttc agggtgtgtg
     3721 catcccaatg gggtgacagt acggattgag tggccagcga tcgggtgtta ccaccggctg
     3781 attcagcagc agccgttcag ccatcccgcc ccgttcccca gctaaatatc agcgccaacc
     3841 ccgccagccc ccacacgatc attggcgccg ccggcagatc ccagtacagc gaacagtaca
     3901 gccccgccag atagccgcac gcacccagtg cccagccggc catcacgcca ccacggccct
     3961 cccaccttcg caccgccagc gccggaacta ccaggctggc aaagaccagg tacacgccca
     4021 ccagttgcac gctggtcgta atcaccaggg cgaacagaac gtagaagccg gcattgccga
     4081 ggtggcgtcc caatgccagc cagagcaggc cacagggaac cagcacaccc gcactccaga
     4141 tgagctgagt cggatccacc cacagaatct ggcccgccag catctctttc agtgcatcgc
     4201 cgccgtgcgg atcctgggcc agcagcaaca gaccgcccgt ggcggccagc acaaacagta
     4261 cgccgatcag cggctcctgc aatgtgccca ttttcctgtc ggtgaacgcc agcagcagcg
     4321 caccacccag cgcggcagca gaagcaaaca gctgcactgc ccaggcggat tcaaaatcca
     4381 gcatgcgtgc cgcaatcaca cccacgcccg cgatctgtgc aatcgccagg tcgatgaaaa
     4441 tgatgccgcg gcgcaccacc tcgcgtccca gcggcacgtg cgtgaacagc accagaatgc
     4501 cggcgaccag cgccggcagg atgatgtcca ggtctgaccc gctcatggtg cggacccgtt
     4561 cagcagctgc agtgtacgcg tgaacagcgt gggcagatcc gcactctgtt catcaccacc
     4621 gaccgtgaac ggcagcacga tcacgggtac ctgcattttt tcgcccagcc agtgcgcggc
     4681 tttggcattt tcaaaagcgg ccaccagaat cgccgccggt ttatcggcag tgagtgtttc
     4741 cagcagacgt ttctgatccg cgccgctggg aggaattccc
//
LOCUS       DASUEG010000678         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_96473, whole genome shotgun sequence.
ACCESSION   DASUEG010000678 DASUEG010000000
VERSION     DASUEG010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96473"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..622
                     /locus_tag="VFX11_12780"
     CDS             59..622
                     /locus_tag="VFX11_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939545.1"
                     /translation="MLIKTYESHGLTCNVSVNAPTTKTGLYAYRGDLVLEEGDVADSS
                     GRRKPPRAVIKQAIALVDRDKLLLMVGALQDLALLPLFAERYRNDFAGGLRALFYVEN
                     LSKPLQVELDGVNYLLLPHDEGAVWNTLMEDMKLDKDDFKGQSAEDKVITMYNAVHGF
                     KPNFGSAGFDEALTHTFVVKKEARGPV"
     gene            complement(745..1158)
                     /locus_tag="VFX11_12785"
     CDS             complement(745..1158)
                     /locus_tag="VFX11_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein"
                     /protein_id="HEX4939546.1"
                     /translation="MNSTRQLIAVAVDRSGNVASHAGRAHRWTVFDAWPNGQLEVAYS
                     IQLAEHACLHEWHVSDFPERHPLHAVDVAIAASGGEGVQRRLGERNTRLLTTAELDPV
                     VAVKQYLMGQLAPGLPHDANGCGGEGHQHAVPAHS"
     gene            complement(1185..1907)
                     /locus_tag="VFX11_12790"
     CDS             complement(1185..1907)
                     /locus_tag="VFX11_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX4939547.1"
                     /translation="MSEMSLNRETALRIALAARILPGVGVGKLLEILHRRISGELNEE
                     SLQTITVTDLKTGFGSADGEEDGEDISIGLPEMKEAVRILWGEASNEQLPQPVALDHI
                     PPRSVRVAVASNSGEKLDGHFGSCIRFLVYQVSVDAVQLVDVRSALEADFSDDKNAFR
                     ANLIHDCQVMYVVSIGGPAAAKVVRAGIYPIKVIEGGMARDVLDKLRGVMGSAPPPWL
                     AKLIGVGAEERVRFERSSDNAA"
     gene            complement(1932..2189)
                     /locus_tag="VFX11_12795"
     CDS             complement(1932..2189)
                     /locus_tag="VFX11_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX4939548.1"
                     /translation="MKIGVVYAGPQQLQLNCNLDDGGTVADAIERSGILRFCPDIDLN
                     VQKVGVYGKFVKLDSVLKDGDRVEIYRKITRVVDDEDDDDD"
     gene            complement(2225..>2559)
                     /locus_tag="VFX11_12800"
     CDS             complement(2225..>2559)
                     /locus_tag="VFX11_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HEX4939549.1"
                     /translation="AYALRNGPVASMLDGLGMGLGFTWALTLLGGIREVLGSGTLFAH
                     ASSLLGPAFHWMELQILPPDTGVLLAILPPGGFIVLGLLIASKRMIDQRIAARQQAPN
                     GPLVMLQS"
BASE COUNT          530 a          816 c          704 g          509 t
ORIGIN      
        1 gtgccggtgg cagcgcaggt tgcataactg atttttccaa tacggagatt gacaccccat
       61 gctgatcaaa acctatgaat cccacggcct gacctgcaat gtcagcgtca acgcacccac
      121 cacgaaaacc ggtctgtacg cctatcgcgg tgacctggtg cttgaggaag gtgatgttgc
      181 cgattcctcg ggccggcgca aaccgccgcg cgcggtcatc aagcaggcta tcgcgctggt
      241 ggatcgtgac aaattgctgc tgatggtggg tgccctgcag gatctggctc tgctgccgtt
      301 gtttgccgag cgctaccgca acgacttcgc cggcgggctg cgggcgctgt tctacgtgga
      361 aaacctgtca aaacccctgc aggttgaact ggacggcgtc aactacctgc tgctgcccca
      421 cgatgaaggc gcggtgtgga acacgctgat ggaagacatg aaactggaca aggatgattt
      481 caagggtcag tcagcggagg acaaagtcat caccatgtac aacgcggttc atggcttcaa
      541 acccaatttt ggcagcgccg gttttgatga agcgttgacg cacaccttcg tggtgaagaa
      601 agaagcgcgc gggccggtgt aacgctgacg caggagcaac cgcttacaga caaaaaattg
      661 ccggcctaca ctcagtcagg ccggcaattt ttttttgctc agagaaaacg gcgctttcga
      721 ctgcaacaag atctgctgaa cggattatga atgcgcaggg actgcgtgct ggtgcccctc
      781 accgccacaa ccattggcat cgtgcggcag cccgggcgcc agttgtccca tcaggtattg
      841 cttcactgcg acaaccgggt ccagctccgc ggtggtcaac agccgtgtgt tgcgctcacc
      901 cagccggcgc tgcacgcctt caccaccact ggcggcaatg gccacatcca ccgcatgcag
      961 cggatgtcgt tcgggaaaat cgctcacatg ccactcatgc aggcaggcgt gttccgccag
     1021 ctggatgctg taggcaactt ccagctggcc attgggccag gcgtcgaaca cggtccagcg
     1081 gtgagcacgg ccagcatggg acgccacatt gcccgaccgg tccaccgcca cggcaatcag
     1141 ttgtcgtgtg ctgttcatgt ctgtcactcc gtaaatggtt gcactcaggc cgcgttatca
     1201 ctgctgcgtt cgaaacgcac acgctcttct gcacccacgc cgatcagctt ggccagccag
     1261 ggcggcggcg ccgagcccat cacaccgcgc agtttatcca gcacgtcccg cgccatacca
     1321 ccctcgataa ccttgatcgg gtaaattccc gctcgcacga ccttggcggc ggccggccca
     1381 ccgatggaga ccacatacat cacctggcaa tcgtggatca ggttggcgcg aaacgcattc
     1441 ttgtcgtcgg agaaatcggc ttccagcgcc gaccgcacat ccaccagctg cactgcgtcg
     1501 acactcacct gatacaccag gaagcgaatg caggaaccaa agtggccgtc cagtttttct
     1561 ccgctattgg atgcaacagc cacacgcacc gagcggggcg gaatgtggtc cagcgcgacc
     1621 ggctgcggca gctgttcatt ggacgcctca ccccacagaa tgcgcacggc ttccttcatt
     1681 tccggcaggc caatactgat gtcctcgcca tcttcttcgc catcagcgct gccgaatccg
     1741 gttttcagat cggtgacggt aatggtctgc aggctttcct cattcagctc gccactgatc
     1801 cggcgatgca ggatttccag cagttttcct acgcccacgc cgggcaggat ccgcgcggcc
     1861 agggcgatgc gcaatgcggt ttcacggttc aggctcatct cgctcatggt tcaccctctc
     1921 tcacatcagg atcaatcgtc atcgtcgtct tcgtcatcaa cgacacgggt tatcttgcgg
     1981 tatatttcca cgcgatcacc gtccttcagg acgctgtcga gtttgacgaa cttgccatag
     2041 acgcccactt tctgcacatt caggtcgatg tccgggcaga aacgcagaat gcccgagcgc
     2101 tcaatggcgt ccgccaccgt gccgccgtca tccagattac agttgagctg caactgttgc
     2161 gggcccgcat acaccacgcc aattttcatg aaggcttcct ccgcaggatg gattttccgc
     2221 gttatcacga ctgcagcatt accagcggac cgttcggcgc ttgctgccgc gctgcaatgc
     2281 gctggtcaat catgcgtttg gacgcgatca gcagtcccag cacgatgaat ccacccggtg
     2341 gcagtatggc cagcaggacg ccggtgtccg gcggcagaat ctgcaattcc atccagtgga
     2401 aagcgggccc aagcaatgaa gatgcgtgcg cgaacaacgt gccgctgccc agcacctcgc
     2461 gaatcccgcc cagcagcgtc agcgcccagg taaaaccgag ccccatgccc aaaccatcca
     2521 gcatcgaggc caccgggccg ttcctcagcg cataggctt
//
LOCUS       DASUEG010000679         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1134673, whole genome shotgun sequence.
ACCESSION   DASUEG010000679 DASUEG010000000
VERSION     DASUEG010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134673"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..259)
                     /gene="rpmG"
                     /locus_tag="VFX11_12805"
     CDS             complement(104..259)
                     /gene="rpmG"
                     /locus_tag="VFX11_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX4939550.1"
                     /translation="MRDKIKLVSSAGTGYFYTTTKNKRTMPEKMEIKKFDPVVRKHVL
                     FKEAKIK"
     gene            complement(271..507)
                     /gene="rpmB"
                     /locus_tag="VFX11_12810"
     CDS             complement(271..507)
                     /gene="rpmB"
                     /locus_tag="VFX11_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX4939551.1"
                     /translation="MAKVCQVTGKRPIVGNNVSHANNKTKRRFEPNLHHHRFWVENEK
                     RFVKLRISSKGMRIIDKLGIDQVLADIRARGEKV"
     gene            743..1396
                     /locus_tag="VFX11_12815"
     CDS             743..1396
                     /locus_tag="VFX11_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4939552.1"
                     /translation="MDKPLAQVLIVDDHPLYLDGVTALLRDLFGAVNILQATTGRDAL
                     AITGSRSDLDWIFLDYTLPDCTGLSLLRTLRENLVTAPVVMVSGADQVALVTEALDLG
                     ASGFIAKTGARDDYRQCLAVIEAGGTYLPAGVAQAVAHYRATVQAERNRIRKQISQRQ
                     NDVLLLIAAGYSNGEIATALGISEHTVKAHVSTLMQLLNADNRVHCIAEARELGLIS"
     gene            complement(1409..3121)
                     /locus_tag="VFX11_12820"
     CDS             complement(1409..3121)
                     /locus_tag="VFX11_12820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX4939553.1"
                     /translation="MNSAIGKRQVDYAFRQLRVTGWPHALAVAITAYVIWRNAPGLDV
                     ILWFFVFQLSHVLRILLLDRWWQAAQTTEEYRRIRRQLIASMVVAGSCWGVVPLLFMD
                     NVSGEMVIFISLVLAGMICATLPALAACLPAYIAFATPIFLALIYHYYSLGLSATAFL
                     SFAFLGAMLAISTTVHRIITGYISLDLENQTLLQEVTRARDDAERANQSKSSFLAAAS
                     HDLRQPLQALGFFLECINVRVGDGDPELRTLARQALGSHESLSDLLNALLELSRLESS
                     ELNVRRQHFLLRHFLQPIVNEFQSLAQAKRLSLVLSGDDAVVETDPVLLARVVRNLLD
                     NAVKFTADGSIVVRLLPEADGVQLTVTDTGVGIADADQHKVFTEYFQVDNEARQASRG
                     LGLGLSIVSRICRLLGHSLRLNSTPGIGTTVVVSLPRGDASHILQAVHPQPLDVAGHC
                     VLVVDDDPVILQALAALLRDWGCTALTATGMDDALACVVAGGQKPDLVLSDYRLDGGV
                     HGIDLIRAVREKIGDDLPALLMSGDTDPALVQSIRQQQFHLLHKPVKPVQLRKVMHQL
                     LASA"
     gene            complement(3273..3947)
                     /gene="radC"
                     /locus_tag="VFX11_12825"
     CDS             complement(3273..3947)
                     /gene="radC"
                     /locus_tag="VFX11_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX4939554.1"
                     /translation="MAITDWPVDERPREKLLQRGGASLSDSELLAIFLRTGIQGKSAV
                     ELARDLLTHFDGLRGVMDATPERFEDVKGMGPAKWVQLQACLELGKRYLETTLDRSDA
                     FTSPAATRQYLRARLRGYHHEVFACLFLDNQNRLIRFEELFTGTIDGASVYPREVVKR
                     VLAHNAAAVIFAHNHPSGVAEPSQADQHITRRLQQALDLVDVRVLDHLVIGDSEVVSL
                     AERGLL"
     gene            4141..>4274
                     /locus_tag="VFX11_12830"
     CDS             4141..>4274
                     /locus_tag="VFX11_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX4939555.1"
                     /translation="MRLANRRIIVGVTGGIAAYKSAELIRRLKDQGADVRAVMTAAAQ
                     "
BASE COUNT          843 a         1340 c         1321 g          770 t
ORIGIN      
        1 atggcagcca tgaagacaac gggagtgctg aagggggcat gtgaacgcaa agagccgggg
       61 aggctcacgc ccacccggct cagcagaaag ccttccaaat caattacttg attttggctt
      121 ccttgaacag cacatgcttg cggacgacgg gatcaaattt cttgatctcc attttttccg
      181 gcatggtgcg cttgttcttg gtagtggtgt agaagtatcc ggtaccggcg ctggaaacca
      241 gcttgatctt gtcacgcatg ggtcaattcc tcagactttt tcgccacggg cgcggatgtc
      301 agccagcacc tgatcgatgc ccagcttgtc gatgatccgc atacccttgg aagatatacg
      361 cagtttcaca aagcgctttt cgttctcaac ccagaaacgg tgatggtgca ggttgggttc
      421 aaaacggcgc ttggttttgt tgttcgcgtg agatacgttg ttgccgacga tcggacgctt
      481 gccggtaacc tgacaaacct tagccattgc cttttctcca aatcggccgc gccgctgacc
      541 ggcacaaccc ttcaatacag tgagactcta cccgagaggg gctgctttat accaaaagcg
      601 gttggtgaaa gcaaccgcgc gggcggatcg ggtcgccggt ttcagccgat ccgtggccgg
      661 gtggacagga ttgccggttg cagcacccca tgaatgaatc gacaatgcgg gccacgctcg
      721 ccgaatggaa tgaccgcccg tcatggacaa accccttgcc caggttctga tcgttgatga
      781 ccacccgctg tacctggacg gcgtgaccgc gctgctgcgg gatctgttcg gagcggtgaa
      841 catcctgcag gccaccaccg gccgggacgc cctggccatc accggcagcc gcagcgacct
      901 ggactggatc ttcctggatt acaccctgcc ggactgcacc ggcctgagtc tgctgcgcac
      961 gctgcgggag aacctggtca cggcgccagt ggtgatggtg tccggcgcag accaggtggc
     1021 gctggtgacc gaagcgctgg acctgggtgc cagtggtttc atcgccaaga ccggcgcccg
     1081 tgacgattac cgtcagtgcc tggcggtgat agaggccggg ggcacctacc tgccggcggg
     1141 cgtggcgcag gcggtggccc actaccgcgc caccgtacag gccgaacgca accgcatccg
     1201 caagcagatc agccagcgcc agaacgacgt gctgctgctg atcgccgccg gctacagcaa
     1261 cggcgagatt gccactgccc tgggcatttc cgagcacacc gtgaaggccc acgtgtccac
     1321 cctgatgcag ctgctgaatg cggacaaccg ggtgcactgc attgccgagg cgcgggagct
     1381 ggggctgatc agctagggtt ggcaggccct aggcactggc cagcagctgg tgcatgacct
     1441 tgcgcagctg caccggcttc accggcttgt gcagcagatg aaactgctgc tggcggatcg
     1501 actgcaccag ggccggatcg gtgtcgccgc tcatcagcag ggccggcagg tcgtcgccga
     1561 ttttttcccg cacagcccgg atcagatcga tgccgtgcac gccgccgtcc agccggtagt
     1621 ccgacagcac caggtccggt ttctgtccgc ctgcaaccac gcaggccagg gcgtcatcca
     1681 ttccggtagc tgtcagcgcg gtgcagcccc aatcccgcag caatgccgcc agcgcctgca
     1741 ggatgaccgg atcatcgtcc accaccagca cacaatggcc ggccacatcc agtggctgcg
     1801 gatggactgc ctgcaggata tggctggcat cgccccgggg caggctgacc accaccgtgg
     1861 tgccaatgcc cggcgttgaa ttcagccgca gcgaatgtcc cagcaggcgg cagatgcggc
     1921 tgacgatgga caagcccaac cccagtccgc ggctggcctg acgcgcctcg ttgtccacct
     1981 ggaaatactc ggtgaacacc ttgtgttggt ccgcatcggc aatgcccact ccggtatccg
     2041 tcacggtcag ttgcacaccg tctgcctcgg gcaacagccg cacgacgatg gaaccgtcgg
     2101 cggtgaattt caccgcattg tccagcaggt tgcgcacgac ccgcgccagc agcaccggat
     2161 cggtttccac cacggcgtcg tcgccggaca gcaccagact gagccgcttg gcctgggcga
     2221 gggactggaa ttcgttgacg atgggctgca ggaaatggcg cagcagaaaa tgctggcgcc
     2281 gcacgttgag ttcgctcgat tccagccgcg acagttccag cagtgcgttg agcagatcac
     2341 tgagggattc gtgggaaccc agcgcctggc gcgccagtgt gcgcagctcc ggatctccat
     2401 cacccacacg cacgttgatg cattccagga aaaatcccag cgcctgcagt ggctgacgca
     2461 gatcgtggct ggcagcggca agaaaactgg atttggactg gttggcacgc tcggcatcgt
     2521 cccgcgcgcg cgtcacttcc tgcagcaggg tctggttttc cagatccagc gaaatatagc
     2581 cggtgatgat gcggtgcacc gtggtgctga tggcgagcat ggcgccgaga aaggcaaagc
     2641 tcagaaatgc ggtggccgac aggcccagcg agtaatagtg atagatcagc gccagaaaaa
     2701 tcggcgtggc aaaggcaatg taggcgggca gacaggccgc cagcgcaggc agggtggcgc
     2761 agatcatgcc ggccagcacc agcgaaatga aaatcaccat ttcgccggac acattgtcca
     2821 tgaacagcaa gggcaccacg ccccagcagc tgcctgccac caccatggac gcgatcagtt
     2881 gccggcggat gcggcggtat tcctcggtgg tctgggctgc ctgccaccag cggtccagca
     2941 gcaggatgcg caacacatgg gacagctgga agacaaagaa ccacaggatg acatccagcc
     3001 ccggtgcgtt gcgccagatg acataggccg tgatggccac cgccagcgca tggggccagc
     3061 cggtcacccg caattgccgg aatgcgtaat ccacttgccg cttgccgatg gccgagttca
     3121 cctggttttc tccgctggaa tggttcgggt tagccttatt tcttgcgccg attgcgcgcc
     3181 tgcactgcaa aggaattgga cgatagtgtc aaccccactt tcgtattacc ggcgcggggc
     3241 ctggcgctga taaccacgtc agttttcagg cctcacaaca gtccccgctc cgccagggac
     3301 accacttcac tgtcgccgat caccagatgg tccagcaccc gcacgtccac cagatccagc
     3361 gcctgctgca gccggcgggt gatgtgctgg tccgcctggc tcggctccgc cacgccggac
     3421 ggatggttgt gcgcaaaaat caccgccgcc gcgttgtggg ccagcacccg tttcaccacc
     3481 tcgcgcgggt agacggacgc gccgtcaatg gtgccggtga acaactcctc gaagcggatc
     3541 agccggttct gattgtccag aaacaggcag gcgaacactt catggtgata gccccgcagc
     3601 cgcgcccgca gatattgccg ggtggcggcg ggactggtga aggcgtcact gcggtcgagg
     3661 gtggtttcca gatagcgctt gcccagttcc agacacgcct gcaactgcac ccatttggcc
     3721 gggcccatgc ctttgacgtc ctcaaaccgc tctggcgtgg cgtccatcac cccgcgcagg
     3781 ccgtcgaagt gggtcagcag atcccgcgcc agttccaccg cgcttttgcc ctgaatcccg
     3841 gtgcgcagga agatggcgag cagctcactg tcggacaggc tggcgccacc ccgctgcagc
     3901 agtttttccc gggggcgttc gtcaacgggc cagtcggtga tggccatagg gggctccttg
     3961 cataacggtc tccttgaaag cattcatccg gaaagccgga cgtccatcaa tagacagcca
     4021 gtcgaaaaag gaagcgtata ctttcaaaat caaatcccgg gtcttatagt cgcaggcatt
     4081 gcgaaatcgc cgggcgcggc ccggtacacg gcattatccg caacggaatc cgaagctatg
     4141 ttgagactgg cgaaccgccg catcatcgtg ggcgttacgg gcggcatcgc tgcctacaag
     4201 agcgcggagc tgatccgccg gctgaaggat cagggcgccg acgtgcgggc ggtgatgacc
     4261 gccgccgcgc agga
//
LOCUS       DASUEG010000680         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1873167, whole genome shotgun sequence.
ACCESSION   DASUEG010000680 DASUEG010000000
VERSION     DASUEG010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873167"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFX11_12835"
     CDS             complement(<1..673)
                     /locus_tag="VFX11_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4939556.1"
                     /translation="MLDTYRKLETPESIDLDIRIAGPLVRALAFLLDTLIRFVIHIGV
                     SVTLVIFGDVGMAATLIFTFALEWFYPVLFEVLLNGQTPGKIAMGIAVVNDDNTPVSW
                     SASIVRNFLRVVDFLPMGYITGLVTITLNRDFKRVGDLAAGTVVIYREKKIPAPQLPE
                     TRAAPPPVALNLQEQRAVLGFAERQQSLTPERRAELANHLQPVLHRQDQPAVDYLFRI
                     ASWLRG"
     gene            complement(683..1093)
                     /locus_tag="VFX11_12840"
     CDS             complement(683..1093)
                     /locus_tag="VFX11_12840"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX4939557.1"
                     /translation="MSVTPHSAAPALPDLQRKARLYSTLTLVHFFLLLALLIIWYLQV
                     WPPSDKTKSILIILNIHLVPLLAFLPGLLKRKPRVHIWLCFFVLLYFCEGVISAFRLP
                     HIVGVLGLLEALLTTGLFVTAMLAARHLTRLQNH"
     gene            complement(1127..1729)
                     /gene="wrbA"
                     /locus_tag="VFX11_12845"
     CDS             complement(1127..1729)
                     /gene="wrbA"
                     /locus_tag="VFX11_12845"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX4939558.1"
                     /translation="MTRILILYYSRTGNVRDMAKWIARGVEKHPGAEAFLRTVPPVTP
                     VTEVAAPSIPEDGAIYATADDLKSCDGLILGSPTRFGNMAAPMKHFIDNTGSLWMSGA
                     LIDKPAGVFTSTSSLHGGQEATLLSMLLPLLHHGMVCAGIPYDQAALSQTQGGGTPYG
                     ASHHAGSDSKRALDPHEIALCQALGERVARLAQALKRRSS"
     gene            complement(1729..2073)
                     /gene="arsC"
                     /locus_tag="VFX11_12850"
     CDS             complement(1729..2073)
                     /gene="arsC"
                     /locus_tag="VFX11_12850"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460200.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX4939559.1"
                     /translation="MTTLYHNPRCSKSREALQLLRDKGIEPDVVLYLETPPDAATLKA
                     LLKKLKLKPRELMRQKEAEYKELGLDNADLSDDQLVDALVRCPRLIERPILVHGSKAI
                     IGRPPEQVLEIL"
     gene            2222..>2996
                     /locus_tag="VFX11_12855"
     CDS             2222..>2996
                     /locus_tag="VFX11_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX4939560.1"
                     /translation="MIQFQALLDRCARHPRIAFIVYVVQSFFRHDCPRSAAYLAFTSL
                     FAVVPMMTVVYSILALVPQLKGMEATMQSFVFEHFVPATGTQLEEYLQSFAQQAANLT
                     SIGVAMLFVTSVLMLRKIETSFNTIWHVAESRKGVNGFLLYWALLTLGPVLMGGAFAV
                     SSYIASLQIIYDMVPLPGTQKLVLGMLPVLMSAVAFSLTYIAIPNTRVPIRHGIIGGV
                     TAALLFDLARQIMTLFIAFFPTYHLVYGAFAAVPIFLVWV"
BASE COUNT          545 a          915 c          905 g          631 t
ORIGIN      
        1 caccccgcaa ccagctggcg atgcggaaca ggtagtccac cgctggctgg tcctgccgat
       61 gcagcaccgg ctgcaggtgg ttggccagtt cggcgcggcg ttccggcgtc aggctctgct
      121 ggcgttcggc aaagcccagc acggcgcgct gttcctgcag attcagcgcc accggcggcg
      181 gtgcggcgcg agtctccggc agttgcggag caggaatttt tttctcgcgg taaatcacca
      241 ccgtgcccgc cgccagatca cccacgcgct tgaaatcccg gttcagcgtg atggtcacca
      301 gcccggtgat gtagcccatg ggcaggaaat ccaccacccg cagaaaattc cgcacgatcg
      361 acgccgacca gcttaccggc gtgttgtcat cgttcactac cgcgatgccc atggctatct
      421 tgcccggtgt ctggccattc aggagcactt caaacagcac cggataaaac cattccagcg
      481 caaaggtgaa gatcagggtt gccgccatgc ccacgtcgcc aaaaatcacc agtgtcaccg
      541 acacaccgat atggatcacg aaccggatca gcgtatccag caggaacgcc agcgcgcgca
      601 ccagtggtcc ggcgatgcga atgtccagat caatgctttc gggggtttcc agtttgcggt
      661 acgtgtccag catgggttgg cttcagtgat tctgcaatcg ggtcagatgg cgcgccgcca
      721 gcatggcggt cacgaacagt ccggtggtga gcaatgcttc cagcagcccc agcacgccga
      781 caatgtgcgg cagccggaac gcgctgatca cgccctcgca aaaatacagc aggacaaaaa
      841 aacacagcca gatatgcacc cggggtttcc gcttcaacag gccgggcaga aaggccagca
      901 gcggcaccag gtggatgttc aggatgatca ggatcgattt ggttttgtcc gacggcggcc
      961 acacctgcag ataccagatg atcagcagcg ccagcagcag aaaaaaatgc accagcgtca
     1021 gcgtgctgta cagccgggcc ttgcgttgca ggtcgggcag ggccggcgct gcagaatgcg
     1081 gagtgacaga catgatgtaa ggtggattcc gtatcaaggg ttgtcgttac gacgaacgcc
     1141 gtttcagtgc ctgcgccagc cgtgcgacgc gttcgcccag ggcctggcac agtgcgattt
     1201 catgcgggtc cagtgcgcgc ttgctgtcgc tgccggcgtg atgactggcg ccataagggg
     1261 tgccgccgcc ctgggtctgc gacagcgccg cctggtcata gggaatgccc gcacacacca
     1321 tgccgtggtg cagcagcggc agcagcatgc tcagcagtgt ggcttcctgg ccgccgtgca
     1381 ggctgctggt ggaggtgaac acgccggccg gcttgtcgat cagcgcgcca ctcatccaca
     1441 gtgaaccggt gttgtcgata aaatgcttca tcggtgccgc catgttgccg aagcgggtgg
     1501 gtgagccgag gatcagtccg tcgcaggatt tcagatcgtc tgccgttgcg tagatggcgc
     1561 cgtcttcggg aatggacggt gctgccactt ctgtcaccgg tgtaaccggc ggcacggtgc
     1621 gcaggaaggc ttccgcgccc gggtgctttt ccacgccacg ggcaatccat ttcgccatgt
     1681 cccgcacgtt gccggtacgg ctgtaataaa gaatcagaat gcgggtcatc acagaatctc
     1741 cagtacctgt tccggtggcc ggccaatgat ggccttgctg ccatgcacca ggatcggcct
     1801 ctcaatcagc ctgggacagc gcaccagggc atcgaccagc tgatcgtcac tcaggtcagc
     1861 gttgtccagc cccagttcct tgtactcggc ttctttctgc cgcatcagct cgcgcggttt
     1921 cagtttgagt tttttcagca gtgccttgag cgtagccgca tccggcggtg tttccagata
     1981 cagcacgaca tccggctcga tgcccttgtc cctgagcagt tgcaacgctt cccgcgattt
     2041 cgaacagcgc ggattgtggt atagggtggt catgggtttc ctgtggctta gcggcggttt
     2101 gcagtgccgg tgtaatggct tattctgtgt gaacggtatt gtaatggttt agcgcgccgg
     2161 agcgaaaccg gcgccggcgc aatcacagct tattcacggc tcaatcacac agggaattcc
     2221 ggtgatccag ttccaggcgt tgctggaccg ctgcgcacgt cacccgcgca tcgcgttcat
     2281 cgtctatgtc gtccagtcct tcttccgtca cgactgcccc cgttccgcag cctatctggc
     2341 cttcacctcg ctgttcgcgg tagtgccgat gatgacggtg gtctattcta tcctggccct
     2401 ggttccgcag ctgaaaggca tggaagccac catgcagagc ttcgtgttcg agcatttcgt
     2461 gcccgccacc ggcacccagc tggaggaata cctgcagagc tttgcccagc aggccgccaa
     2521 tctcaccagc atcggcgtgg ccatgctgtt cgtgacctcg gtgctgatgc tgcgcaagat
     2581 cgagacctcg ttcaacacca tctggcacgt ggcggaatcc cgcaagggcg tgaacggctt
     2641 cctgctgtac tgggcgttgc tgaccctggg cccggtcctg atgggtggcg cttttgcggt
     2701 atcgtcctat atagcgtcac tgcagatcat ctatgacatg gtgccgctgc cgggcaccca
     2761 gaaactggtg ctggggatgc tgcctgtatt gatgagcgcg gtggcgtttt cgctcaccta
     2821 catcgccatt cccaataccc gggtgccgat ccggcacggc atcatcggtg gtgtcaccgc
     2881 ggcgctgctg ttcgatctgg cgcgccagat catgacgctg ttcattgcct tctttcccac
     2941 gtatcacctg gtgtacggtg cgtttgccgc cgttccgatc ttcctggtgt gggtgc
//
LOCUS       DASUEG010000681         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1551262, whole genome shotgun sequence.
ACCESSION   DASUEG010000681 DASUEG010000000
VERSION     DASUEG010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551262"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX11_12860"
     CDS             <1..541
                     /locus_tag="VFX11_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4939561.1"
                     /translation="AGRLDYDSEGLLLLTGNGPLQHLLTDPKHKLPKTYRVQVEGDIS
                     EHALHQLRIGVELKDGKTRPALANRIQPPADLWERNPPIRFRAEIPTSWLELTITEGR
                     NRQVRRMTAAVGFPTLRLVRVAIGPWSLEGLAPGQSRWSRVPTSILAQLKSAAPQSKI
                     GKKPGKKRINPAHQRSRAR"
     gene            complement(517..969)
                     /gene="moaE"
                     /locus_tag="VFX11_12865"
     CDS             complement(517..969)
                     /gene="moaE"
                     /locus_tag="VFX11_12865"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEX4939562.1"
                     /translation="MAIHIQIQHDDFDLTLEYQRLRRENPQAGAIVTFTGLVRDFNAE
                     GEVNGIVLEHYSGMTEKTLQRIADEAVARWGVAGLTIIHRVGALAVAEQIVLVAVASR
                     HRSEGFAAAEFIMDFLKTQAPFWKRERGPEGEQWVDAKDSDQRARERW"
     gene            complement(970..1221)
                     /gene="moaD"
                     /locus_tag="VFX11_12870"
     CDS             complement(970..1221)
                     /gene="moaD"
                     /locus_tag="VFX11_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX4939563.1"
                     /translation="MIKVLFFARIRDELGRAEMKVEMPEGVHDLAGFTRALIMANPSF
                     EPALTQPNVLTAVNQEMATPVTTIEDGDEIAYFPPVTGG"
     gene            complement(1218..1709)
                     /gene="moaC"
                     /locus_tag="VFX11_12875"
     CDS             complement(1218..1709)
                     /gene="moaC"
                     /locus_tag="VFX11_12875"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX4939564.1"
                     /translation="MTELTHLDQQGNARMVDVTEKAITQREATAEARVTMLPETLAMI
                     TEGRHKKGDVLAVARIAGIQAAKKASDLIPLCHPLMLTSVKVELTADMVHHQILIRAT
                     CKLAGQTGVEMEALTAASIAALTIYDMCKAVDKGMTIEGVRLLEKIGGKSGHWQADEG
                     IQS"
     gene            1850..>2132
                     /locus_tag="VFX11_12880"
     CDS             1850..>2132
                     /locus_tag="VFX11_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939565.1"
                     /translation="MEQPAFGNELRFFGYEIDAALRDRLTANLEQILAGGPADRLAKL
                     AAQDLVELVDVALRSYYKQPADMISLSPLIRKATDTGMSAVSKAVDMVIH"
BASE COUNT          418 a          647 c          614 g          453 t
ORIGIN      
        1 tgccgggcgt ctggattacg attccgaagg gttgctgttg ctgaccggaa atggccccct
       61 gcagcatctg ctcacggacc ccaaacacaa gctgccaaag acctatcggg tgcaggtgga
      121 aggcgacatt tcggagcacg ccctgcacca gctaaggata ggagtcgagc tgaaggacgg
      181 caaaacccga ccagcccttg caaaccggat ccagccacct gccgatctgt gggagcgcaa
      241 tccaccgatc cggtttcgcg ctgaaattcc cacttcctgg ctggagctga ccatcaccga
      301 aggccgcaac cggcaggtcc gacgcatgac cgccgccgtg ggttttccaa ccctgcgcct
      361 ggttcgcgtc gccatcggcc cctggtcttt ggaaggtctg gcacctggac agtctcgctg
      421 gagcagagtg ccgacgtcca ttctcgccca actgaaatcc gctgcacccc agtcaaaaat
      481 cggcaaaaaa cccggaaaaa aacggatcaa tcccgctcac cagcgttctc gcgcccgctg
      541 atccgaatcc ttggcgtcca cccactgctc tccttctgga ccacgctccc gcttccagaa
      601 cggtgcctgg gttttcagga agtccatgat gaattccgct gcggcaaatc cctcactccg
      661 atgacgcgac gccaccgcca ccagcacaat ctgctcggct accgcgagcg caccaacgcg
      721 atggatgatg gttagacccg ccacacccca gcgcgcgacc gcctcatccg ctatgcgttg
      781 gagtgtcttt tccgtcatgc cggagtagtg ctccaaaacg atgccgttca cctcaccctc
      841 tgcattgaaa tcgcgcacga gaccggtgaa ggtcacaatc gcccctgcct gcgggttttc
      901 ccggcgcaag cgctgatatt cgagggtcag gtcgaaatcg tcgtgctgga tttgaatgtg
      961 gatggccatt cagcctccag tgaccggcgg gaagtatgca atttcatcgc catcttctat
     1021 cgtcgtgacc ggtgttgcca tttcctggtt cacggcggtg agcacgttgg gctgggtcag
     1081 tgcgggctca aaggaaggat tggccataat aagcgcacgg gtgaacccgg cgaggtcgtg
     1141 cacaccttca ggcatttcaa ctttcatttc cgcgcggccc agttcatccc ggatgcgggc
     1201 gaaaaacagt accttgatca tgattggatt ccttcgtccg cttgccagtg tccgctcttg
     1261 ccgccgatct tttccagcag gcggacacct tcgattgtca tgcctttgtc cacggccttg
     1321 cacatgtcgt agatcgtgag tgcggcaatg ctggcagccg tcagcgcttc catttcaacg
     1381 ccggtctggc cggccagttt gcaggtggcg cggatcagga tctggtggtg caccatatca
     1441 gctgtcaatt ccaccttcac cgaggtgagc atgagcgggt ggcacagggg gatcaggtcg
     1501 gaggcttttt tggcagcctg gatgccggcg atacgggcca ctgccagcac gtctcccttt
     1561 ttgtggcgcc cttcggtgat catggccagg gtttcgggca gcatggttac ccgggcctca
     1621 gcagtggctt cgcgctgggt gatggctttc tcggttacat ccaccatccg ggcgttaccc
     1681 tgctggtcga ggtgggtcaa ttctgtcatg ttgctcacaa tcaggaggat gtgtcgtaca
     1741 ggcgacgcaa tcggtaagcc ggcgcccttt tcctggccag cagtcgtcca ttattattcg
     1801 acctgccagt ttcagcacac aatacataaa gatgggtagg gaaggcaaaa tggagcagcc
     1861 agcatttggt aacgaactgc gatttttcgg ctatgagatc gacgctgcgc tgagggaccg
     1921 gctgacggcg aacctggagc agattctggc aggtggaccg gcagaccggc tggcgaagct
     1981 ggcagcgcag gatctggtgg agctggtgga cgttgcgctt aggtcttatt acaagcagcc
     2041 ggcagacatg atctccctct cccccctgat ccgcaaagcg acggataccg gcatgtcggc
     2101 cgtatccaag gcggtggaca tggtgatcca cc
//
LOCUS       DASUEG010000682         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1994821, whole genome shotgun sequence.
ACCESSION   DASUEG010000682 DASUEG010000000
VERSION     DASUEG010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994821"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFX11_12885"
     CDS             complement(<1..465)
                     /locus_tag="VFX11_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX4939566.1"
                     /translation="MKAGRILLSPRGISAWITLAAGLLMTTLLTSWLYENRQENAAAR
                     FGLIAADVINSIEKRLNDHEQILLGGAGLFDATGTVSRQQWHAYVSRLRLEYHYPGIQ
                     GVGFSQAIAPADLPRHIEEIRNSGFPDYQIRPLGERTLYTAIIYLEPFAGRNL"
     gene            complement(462..>2127)
                     /locus_tag="VFX11_12890"
     CDS             complement(462..>2127)
                     /locus_tag="VFX11_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018876986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939567.1"
                     /translation="IDLIRQGETLKQASRMGELITRAQSEFIGSSDWRQSFKTLLDEI
                     LALTGSEYGFIGEILRTASGDPYLKTYAITNIAWNAATRAFYEANAPTGMEFFNLKTL
                     FGAALTNGSPVIANNPAQDPRRGGLPEGHPPLNAFLGLPVHYGQEMVAMVGLANRSGG
                     YDQTLVDFLRPLTATLGQLVDAARIRQALEQQQAHIRHLLGNLPGTVYRCANDVHWTM
                     HFMSDYVRTLTGYPASDFLPAQGGTRTFASLIHPDDAPMVNDSVQNDLRNQRGFRIEY
                     RIQHADGQYRWVQELGRGVFQQDGSLLYLDGFIWDVTDRKRLEQMKDEFISTVSHELR
                     TPLTSISAALGLLQGGALGPLQDNQRELIAIAHRNSHRLSLLINDLLDMEKLAAGKMP
                     FNIQLQSLLPLVQEALEANRTYGYERKVSLTLKPDSPNVSVAVDSQRLMQVMSNLLSN
                     AIKFSPENGLVEIGISLADNSARVSVVDHGPGIPAAFQDRIFQKFAQADASDSRRQGG
                     TGLGLAITRELVERMGGQIGFLSQEGSGTTFFVDLPMATPLNEEAA"
BASE COUNT          382 a          615 c          684 g          446 t
ORIGIN      
        1 cagattgcgc ccggcaaagg gttccaggta gatgatggca gtgtagagcg tgcgttcgcc
       61 cagcggccgg atctggtaat caggaaagcc gctgttgcgg atttcctcga tatggcgggg
      121 caggtcggcg ggtgcaatcg cctggctgaa accaacaccc tggatgcccg gataatggta
      181 ttccagacgc agccgggaga cgtaggcatg ccattgctgg cgggaaaccg tgcccgtcgc
      241 atcaaacagg ccggcgccac cgagcaggat ctgctcgtgg tcgttcagcc gtttttcaat
      301 actgttgatc acatccgctg ctatcagccc gaagcgggcg gcagcatttt cctgccggtt
      361 ctcgtacagc cagctggtca gcaatgttgt catcagcagg ccggccgcca gggtgatcca
      421 ggcggaaatt ccgcgtggcg agagcaggat gcgacccgct ttcatgccgc ttcctcgttg
      481 agcggtgttg ccatgggcag atcgacaaag aaggtggtgc cggacccttc ctgtgacagg
      541 aatcctattt gccctcccat ccgttccacc agttcccggg tgatggccag acccagtccg
      601 gtgcccccct gtcgccggga atcggaggca tcggcctggg cgaacttctg gaagatgcgg
      661 tcctggaatg cggcgggaat gccagggccg tgatcgacga ccgatacccg ggcgctgttg
      721 tctgccaggc tgatgccgat ctccaccagc ccgttttccg gtgagaattt gatggcgttg
      781 gagagcaggt tcgacatcac ctgcatcaac cgctggctgt ccaccgctac tgaaacgttc
      841 ggtgaatccg gcttcagtgt cagcgagacc ttgcgttcgt agccgtaggt gcggttggct
      901 tccagtgcct cctgtacaag gggcaacagg gattgcagct ggatgttgaa gggcattttt
      961 cctgcggcca gtttttccat gtccagcagg tcgttgatca gcaggctgag acggtggctg
     1021 ttgcggtggg caatggcgat cagctcccgc tggttgtcct gcagcgggcc cagtgcgccg
     1081 ccctgcaata atcccagcgc tgctgaaatg gaggtcaggg gtgtacgcag ttcatggctc
     1141 acggtggaga tgaattcatc tttcatctgc tccaggcgct tccggtcggt gacgtcccag
     1201 atgaatccat ccagatacag caggctgccg tcctgctgga acacgccgcg ccccagttcc
     1261 tgcacccagc gatactggcc gtcggcgtgc tggatgcgat attcgatgcg gaaaccccgc
     1321 tggttacgca agtcgttctg aacgctgtcg ttgaccatgg gtgcgtcgtc ggggtggatc
     1381 aggctggcaa acgttcgggt gccgccctgt gccggcagaa agtcagacgc cgggtagccg
     1441 gtgagggtgc gcacgtaatc actcatgaag tgcatagtcc agtgtacgtc attggcgcaa
     1501 cgatagaccg tgcccggcag gttgcccagc agatgacgga tgtgggcctg ctgctgttcc
     1561 agtgcctggc ggatgcgggc ggcgtccacc agctggccca gggtggctgt cagggggcgc
     1621 agaaagtcga ccagggtctg atcgtatccg cccgaccggt tcgccagccc caccatcgcc
     1681 accatttcct ggccgtagtg caccggcaga cccagaaaag cgttgagcgg cggatgccct
     1741 tcgggcaatc ccccgcggcg gggatcctgc gccgggttgt tggcgatgac cgggctgccg
     1801 ttggtcaagg cggcaccgaa cagggttttc aggttgaaga actccattcc cgtaggcgca
     1861 ttggcttcat aaaatgcgcg ggtggcggca ttccaggcga tattggtaat ggcgtaggtt
     1921 ttcagatacg gatcgccgct ggcggtacgg aggatctcac cgataaagcc gtattcactg
     1981 ccggtcagcg ccagtatttc atccagcagg gttttgaacg actgccgcca gtcgctgctg
     2041 ccgatgaatt cggattgcgc acgggtaatg agctcgccca tccggctggc ctgcttcagt
     2101 gtttccccct gccgaatcag gtcgatc
//
LOCUS       DASUEG010000683         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1929926, whole genome shotgun sequence.
ACCESSION   DASUEG010000683 DASUEG010000000
VERSION     DASUEG010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929926"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..2351)
                     /locus_tag="VFX11_12895"
     CDS             complement(339..2351)
                     /locus_tag="VFX11_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939568.1"
                     /translation="MPTANHVEMGLPGVGLEHGNFYANGFPTDLRRDDQGNIDIDDFP
                     RQVHWLTKRYVKAIQEMANGYHTVMPVYLPFAAAIDLDAMSDNDMDYTDPQSPVQLID
                     VDIESPEYGRRFPLQLSQTKIPDTYRPKHLLQVIPTLGINLRPNTTYALIVSNAAPLT
                     GLNYWEQHPQLSAVLIPSRSEVPLSDIVNAVYEPLRNYLTLHNIDPGSIVGATVWTTG
                     DPLLRFHQASEQVAMRAEKLPELPISELDTLDDYPGYCVIRGFVDLPSYQKGVAPYWL
                     SGGEIEWNANGEPLEQRSRRAEFVLTIPKFTPMPAAGFPLLSYVHGASGNARQVYDRG
                     EFDHVDLAHYPYYIGKAGEGPAQIAAERGWASSGLAGHTARDHIGQVRSLFGTSVYNL
                     FNPVGSLGVYQTMAWERIYFRRIIERIEVNRALCPDADPGPGQTHFRIDPDMEVNLGQ
                     SQGNWVSSLMVAADPRPYQGVIFSGAAGTWTRLFNNNPGFELTMNTAVINRIPVLNLD
                     DAHPFLMLMEWLLGPVEAATNIETLMRYPTKTPPHVIGFSGYNDYLLPEATQRPFFMA
                     LGSDLVGEDVGASARRTFMPHISIAGAQQLAYPVSNNFQVPGYGPRINVVMRYRGDNP
                     ALLYNGHEVLFQSDAVKHQYGCFLQHLAAGIAPVVTEGFVQGDPCL"
     gene            complement(2747..4348)
                     /locus_tag="VFX11_12900"
     CDS             complement(2747..4348)
                     /locus_tag="VFX11_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939569.1"
                     /translation="MKNKKRNLNPNAPLLHADHARPKTRRDFIRQGLLTGAATVLGPS
                     LLGLFANPRQAYAQLAGDINTLRTNCGITVQGAGKIPFICFDLAGGANIAGSNVLVGQ
                     QGGQEDFLTTAGYSKLGLPGDMVPAITTVQNTTTTAFINNQLGLLFHTDSAFLRGILN
                     KASPAALAMTDGAVIPARSENDTGNNPHNPMYGIWKAGADGELLTLVGSRPSDSGGNS
                     MAPAMMMDPSVRPTKIDRPSDVTGLVDTGKLVGLLDQADVVNVMESIARISEQKMTKL
                     DPIAGVTVSREDTIRELVRCGYVKSADLADRYGNPATLNPVADVDIVGAAGIFSTNDF
                     NSDGEFQKTASVMKLVVNGYSGAGCITMGGFDYHTGDRAVGEERDFRAGQCMGACIEY
                     AHRVGVPLMLYVFSDGSVASNGMVDDSAEGRGKGVWTGDNQSTAASFYLVYNPGGRPA
                     LIGGDIARRQIGWFRGNGDVETASSPAANNVNLLVETVALNYMALHGDQAQFTTRFPG
                     QGLGNGTMRDSLLSFNPIVNGVIGS"
BASE COUNT          986 a         1257 c         1240 g          902 t
ORIGIN      
        1 ttatcaagtg gcaacaggct ttctcgacca ggcacaacgg agaatctgga ccagtgaccg
       61 tggccctccg aaaaacaacc gatttcaaaa gtaggatttt ctcgtgctct agcacacagg
      121 agatcctgca tgaatgggtg aaaatttacc gatagccaag gccggcgcgg ccgccatcga
      181 tagcgggcga gcgcattatt ttgcggtatt tgttacttga aggaggcgcg tggagcagca
      241 agtgccgtac aagctccact tcactttgat cttgtgactc ggcgaagaag attctaagcc
      301 gtataaaacg gcagtcatgg tagccgccag cgcagacgtt acaggcatgg atcaccttgc
      361 acaaaacctt ccgtgacaac aggcgcgatt ccagcggcca aatgctgcag aaagcatccg
      421 tactgatgtt tgacagcgtc tgactggaac agcacttcgt gaccgttgta cagcaacgct
      481 ggattgtcgc cacgataccg catcaccaca ttgatacgcg gaccatagcc gggcacctgg
      541 aaattattcg aaaccggata ggccagctgt tgcgccccag cgatgctgat gtgaggcatg
      601 aacgtgcgcc gggcggacgc accaacatcc tcaccgacaa gatctgatcc cagcgccatg
      661 aagaatggcc gctgcgtggc ttccggcaac aggtagtcat tgtagccgga aaaaccaatc
      721 acatgcggcg gcgttttggt gggatagcgc atcagggttt caatgttcgt cgcagcttca
      781 accggaccca gaagccattc catcagcatc agaaacgggt gtgcatcatc aaggttgaga
      841 accggtatcc ggttgatgac ggccgtattc atcgtcagct caaaaccggg attgttgttg
      901 aacagtcgag tccaggtacc agcagcaccg gaaaaaatga cgccctgata aggacgtggg
      961 tcggcggcca ccatcaacga gctgacccag ttgccctggg attgccccaa attcacttcc
     1021 atgtcgggat caatacggaa atgcgtttgc cccggccccg gatcggcgtc gggacacagc
     1081 gcgcggttca cttcaattcg ctcaatgata cggcggaaat aaattcgctc ccacgccatc
     1141 gtctggtaaa cgccaagcga gcctacggga ttgaacaggt tgtagaccga cgtaccaaac
     1201 agactgcgaa cctggccgat atgatcgcgg gctgtatgcc ccgccagccc gctgctggcc
     1261 cagccacgct ctgctgcaat ctgcgcgggg ccttcacccg ccttgccaat gtaatagggg
     1321 taatgggcaa gatcgacatg atcgaattca ccgcgatcgt acacctgccg ggcattgccg
     1381 ctggcaccgt gcacatagga caacaacgga aatcctgcag ccggcattgg cgtgaacttg
     1441 ggaatggtca gcacaaactc ggcgcgacgg gagcgctgtt ccagcggctc tccgttcgca
     1501 ttccattcaa tttcaccacc ggacagccag tacggcgcca cacctttctg atagctcggc
     1561 aaatccacaa agccgcgaat tacacaatag ccaggataat catccagcgt atccagttca
     1621 ctgatgggca attcgggcag tttttctgcc cgcattgcta cctgttcaga cgcctggtga
     1681 aagcgcaaaa gcggatcgcc ggtggtccag acggtggcac caacgatact gcctggatcg
     1741 atgttgtgca gtgtcagata gttgcgcaag ggctcataga cggcattgac gatatcggac
     1801 agcggcacct cactgcgcga gggaatcaat acggcagaca gttgcggatg ctgctcccaa
     1861 taattcaatc ctgtgagcgg tgcagcgttt gacacgatca acgcataagt ggtgttgggg
     1921 cgcaaattga tgccgagcgt gggaattacc tgcagcagat gttttggccg ataagtgtca
     1981 ggtattttgg tttgcgagag ctgcagagga aagcgtcgtc catactccgg tgattcgata
     2041 tcgacatcaa tcaattgcac cggcgactgc ggatcggtgt aatccatatc gttgtcggac
     2101 atggcatcca ggtcgatcgc cgctgcgaac ggcaagtaca ccggcatgac agtatgatag
     2161 ccgttcgcca tctcctgaat agccttgacg tagcgctttg tcagccaatg gacctggcgg
     2221 ggaaagtcgt cgatgtcgat gttgccctga tcgtcgcgac gcaaatcggt gggaaacccg
     2281 tttgcgtaga aattgccgtg ttccaatcca acgccaggca accccatttc cacgtggttg
     2341 gccgtcggca ttttgcaacc atatagtgtt gccagcaaca ccgtgatgta gcaaacagct
     2401 cgacctggcc tcatcgaaaa gggctcccgg attatttttg ttattcaaac gtgcggacgt
     2461 catctgactg atcagaataa agtgacaccc aaacgtccag actttgctag ttatgctagc
     2521 gcgggggata tgccagatct tgtaagtatc ggcctttgat gggaaggtat gagacgaatg
     2581 ctggccggtt tgtccggaga ccgtctgtaa acagccgttt ggtgcaggta ttggctttca
     2641 gcgtatgctg atatgcctgg ccagagcaga gtgacacaaa cacaaaaaaa cccggcgaac
     2701 cgggccttct tgtgctgctg atttacgaga gctcagatgc gtgccatcag ctgccgataa
     2761 cgccattcac gatcggattg aacgacaaca ggctgtcacg catggtgcca ttgcccaggc
     2821 cctggcccgg gaaacgtgtg gtgaactggg cctgatcgcc gtgcagcgcc atgtaattca
     2881 gcgcgaccgt ttccaccagc agattgacgt tgttggcagc ggggctggat gcggtttcca
     2941 catcgccgtt gccacggaac cagccgatct gacgacgggc aatatcgcca ccgatcagcg
     3001 ccggccggcc accggggttg tacaccagat agaacgaagc ggcggtggac tggttgtcgc
     3061 cggtccagac gcccttgcca cggccttcag cggaatcatc caccatgccg ttgctggcca
     3121 ccgaaccgtc gctgaacacg tacagcatca acggcacgcc cacgcgatga gcatattcga
     3181 tacaggcgcc catgcactgg ccagcacgga aatcccgttc ttcacccact gcacgatcac
     3241 cggtgtggta gtcgaaaccg cccatggtga tacagccggc gccggaatag ccgttcacca
     3301 ccagtttcat caccgacgct gttttctgga attcaccatc gctattgaaa tcattggtgg
     3361 aaaaaatgcc ggcagcgccg acgatatcca catcggccac cggattcagc gtggccggat
     3421 taccataacg gtccgccaga tccgcactct tcacatagcc gcagcgcacc agctcgcgaa
     3481 tcgtgtcttc gcgactgacg gtcacgccgg caatcgggtc cagcttcgtc atcttctgct
     3541 cgctgatgcg ggcgatggat tccatcacgt tcaccacatc agcctgatcc agcagaccca
     3601 ccagcttgcc ggtatccacc aggccggtca cgtcgctggg acggtcgatc ttggtaggac
     3661 gcacgctggg gtccatcatc atcgccggtg ccatggagtt gccgccggaa tccgacggac
     3721 gcgatccgac cagggtcagc aactcgccat cggcgcccgc tttccagatg ccgtacatgg
     3781 ggttgtgcgg attgttgccg gtgtcgtttt cggaacgggc cggaatcaca gcaccgtccg
     3841 tcatcgccag cgcagccggc gatgccttgt tcaggatgcc gcgcagaaag gcactgtcgg
     3901 tgtggaacag caggcccagc tggttattga tgaaggcagt ggtggtggtg ttctgcacgg
     3961 tggtaatggc aggcaccatg tcaccgggca ggcccagctt gctgtaaccg gcggtggtga
     4021 gaaaatcttc ctgtccgccc tgctgcccca ccagcacgtt cgagccggcg atattggcac
     4081 cgcccgccag atcgaaacag atgaacggga ttttgccggc accctgcacg gtgataccgc
     4141 agttggtgcg cagcgtgttg atgtcaccgg ccagttgcgc ataggcctga cgcggattgg
     4201 cgaacaggcc cagcaggctg gggcccagca cggtggccgc gccggtcagc aacccctgcc
     4261 ggatgaaatc gcgacgggtt ttcgggcgcg cgtgatccgc atgcagcaat ggcgcgttgg
     4321 gattcagatt ccgctttttg tttttcatgg tcataccctc aaaatccggt cccgttactg
     4381 cacca
//
LOCUS       DASUEG010000684         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_492497, whole genome shotgun sequence.
ACCESSION   DASUEG010000684 DASUEG010000000
VERSION     DASUEG010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492497"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFX11_12905"
     CDS             <1..477
                     /locus_tag="VFX11_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX4939570.1"
                     /translation="TWLLIDSATATLSLMRGDRVLARYGDVAFGRLGTKPVHYKGDTS
                     TPTGEFRIDGINHASQFHRFFSLNYPTEAHAKKALDQRRITFEQYMEILKAEHAGRRP
                     PYDTPLGGMIGIHGLGPVRLDLHRKYNWTRGCVALDNGQIDALTPHLYIGMRVVIR"
     gene            complement(549..845)
                     /locus_tag="VFX11_12910"
     CDS             complement(549..845)
                     /locus_tag="VFX11_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HEX4939571.1"
                     /translation="MKKQMMVAVMLMAGGLAGCATTSDLEAVKAMAQQAQADAARAQQ
                     TADNALTAANQAADKAQAAEAAANAAASRADAAEASANQVNEKIDRMFKKSMMK"
     gene            1243..2838
                     /locus_tag="VFX11_12915"
     CDS             1243..2838
                     /locus_tag="VFX11_12915"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX4939572.1"
                     /translation="MSNYSANIDQAATVVSANGPSWNAISPEYVARMRLQNRFKTGLD
                     VAKYTAKIMREDMAAYDKDSSQYTQSLGCWHGFIGQQKLIAIKKHHGTTKRRYLYLSG
                     WMIAALRSEFGPLPDQSMHEKTSVANLIEELYTFLRQADARELGGLFRELDAARAAGD
                     AGKEKDVLNKIENFQTHVVPIIADIDAGFGNAEATYLLAKKMIEAGACCIQIENQVSD
                     EKQCGHQDGKVTVPHEDFLAKIRAVRYAFMELGVDDGVIVARTDSLGAGLTKQIAYTK
                     EPGDLGDQYNSFLDCEEVSLANMKNGDVVLNRNGKLLRPKRLPSNLFQFRAGTGEDRC
                     VLDCITSLQNGADLLWIETEKPHVGQIKGMVDRIRAVIPNAKLVYNNSPSFNWTLNFR
                     QQVFDAWQKEGKDVSQYDRAKLMSVDYDSTELALEADRRIQHFQADGSRQAGIFHHLI
                     TLPTYHTAALSTNDLAKGYFGEEGMLAYVAGVQRKEIRQGIACVKHQNMSGSDIGDDH
                     KEYFAGEAALKAAGKDNTMNQFS"
BASE COUNT          685 a         1004 c          882 g          595 t
ORIGIN      
        1 acctggctgc tgatcgacag cgccactgcc accctcagcc tgatgcgggg cgacagggta
       61 ctggcccgct acggcgacgt ggctttcggc cggctgggca ccaagcccgt ccattacaag
      121 ggcgacacca gcacccccac gggggaattc cgcattgacg gcatcaatca cgccagccag
      181 ttccaccgct ttttcagcct gaattatcca accgaggccc atgccaagaa agccctggac
      241 cagcgccgga tcacgttcga gcagtatatg gaaatcctga aggcggaaca cgcaggacgc
      301 cggccaccct atgacacacc gctgggcggg atgatcggca ttcatggact gggaccggtg
      361 cgcctggacc tgcatcgcaa gtacaactgg acccggggct gcgtggcact ggacaacggc
      421 cagatcgacg ccctgacccc gcatctttat atcggcatgc gggtggtgat ccgctaacga
      481 aaaaggcacg gggacaggcc gcaacccgtc cgccgtgcct tcagtgccgt cccgccggga
      541 cagcgcgctt atttcatcat cgatttcttg aacatgcggt cgatcttctc gttcacctga
      601 ttggcagacg cctcggccgc atcggccctg gacgcagccg cgttggcagc cgcttcagcg
      661 gcctgcgcct tgtctgccgc ctggttggca gctgtcagcg cgttatccgc cgtctgctgg
      721 gcacgggcgg catcagcctg cgcctgttgc gccatcgcct tcactgcttc cagatcggac
      781 gtggtggcgc atcccgccaa accgcccgcc atcaacatca ccgctaccat catttgcttt
      841 ttcatggtca aacccccgat tcatccgttg tggaacttga ggatccagag agtaacaccc
      901 gaccggggca tgacacaggt gcccaccggc cggattggcc ccctttcgaa ccggtttatg
      961 acccggatca aattgacgcg ggcgccagcg gcaacaatga ccggcgggtc caatcctgct
     1021 tccccgcctg gacagcgcgc gtccaggcgg gctgcaagtc aggggcgacg cgggctgcag
     1081 acatcattca cgcaaaaaat agcgagtata tgaatcattg atttctttta tgcccgttca
     1141 atccatagaa tgcgcccatc tgtggtctgg ggcgtgcata gcagcaccgg ctcgtcaacg
     1201 gacactgagc aaaacccgtt acctccaact agaggattca ccatgtccaa ctactccgcg
     1261 aacatcgacc aggccgcgac cgttgtgtcg gccaacggcc ccagctggaa tgcgatcagc
     1321 cccgaatacg tcgcccgcat gcgtctgcag aaccgcttca aaaccggtct ggatgtcgcc
     1381 aagtacactg ccaagatcat gcgcgaagac atggctgcct atgacaaaga ctcttcccag
     1441 tacacccagt ccctgggctg ctggcacggc ttcatcggtc agcagaaact gatcgccatc
     1501 aagaagcatc acggtaccac caagcgccgc tacctgtacc tgtctggctg gatgatcgcg
     1561 gccctgcgct ccgagttcgg cccgctgccc gaccagtcca tgcacgagaa aacctccgtt
     1621 gccaacctga tcgaagaact ctacaccttc ctgcgccagg ctgacgcccg cgagctgggc
     1681 ggcctgttcc gcgaactgga tgcggcccgc gctgctggcg acgccggcaa ggaaaaagac
     1741 gttctgaaca agatcgaaaa cttccagacc cacgtggtgc cgatcattgc cgacatcgac
     1801 gccggtttcg gtaacgcgga agcgacttac ctgctggcca agaaaatgat cgaagcgggt
     1861 gcctgctgca tccagatcga aaaccaggtg tctgacgaga agcagtgcgg tcaccaggac
     1921 ggcaaggtaa ccgttcccca cgaagacttc ctggccaaga tccgcgccgt tcgctacgcg
     1981 ttcatggagc tgggcgttga cgacggtgtg atcgttgccc gtaccgattc cctgggtgcc
     2041 ggcctgacca agcaaatcgc ctacaccaaa gagccgggcg acctgggtga ccagtacaac
     2101 tcgttcctgg attgcgagga agtgtcgctg gccaacatga agaatggcga cgtggtactg
     2161 aaccgcaatg gcaaactgct gcgtcccaag cgcctgccgt ccaacctgtt ccagttccgc
     2221 gctggtaccg gcgaagaccg ctgcgtactg gactgcatca ccagcctgca aaacggcgcc
     2281 gacctgctgt ggatcgaaac cgagaagccg cacgttggcc agatcaaggg catggttgac
     2341 cgcatccgcg ccgtgattcc gaacgcgaaa ctggtttaca acaacagccc gtccttcaac
     2401 tggaccctga acttccgtca gcaggtattc gatgcatggc agaaagaagg caaggatgtt
     2461 tcccagtacg accgcgccaa gctgatgagc gtggattacg acagcaccga actggcactg
     2521 gaagccgatc gccgcatcca gcacttccag gcggatggtt cgcgtcaggc cggcattttc
     2581 caccacctga tcacgctgcc gacttaccac actgctgccc tgtccaccaa cgacctggcc
     2641 aagggctact tcggcgagga aggcatgctg gcgtatgttg caggtgttca gcgcaaggaa
     2701 atccgtcagg gtatcgcttg cgtgaagcac cagaacatgt ccggctccga catcggcgac
     2761 gatcacaagg aatactttgc tggcgaagct gcgctgaaag cggcgggcaa agacaacacc
     2821 atgaaccagt tctcctaagc attattggtt gatggaatga agaaggcggg gccctgcggc
     2881 cccgcctttt ttgtgggcca ctgattcagg gggttacaaa agtcgcattt acatttcccg
     2941 ccgtttcggt aaaacggtca gcggttccac cacgcggctg actgcaacga gtggcacccc
     3001 ggaaagtgtc ttcatccggt aatgaattca caggtccacc cgtcgttcgt caggattcaa
     3061 tatggaagtt atggccatca agcgttcgcg cagcgcccga aaggtgattt cgacaacagc
     3121 ccggattgcc agctgtctac atttagtaat ttttctgctg gccgcc
//
LOCUS       DASUEG010000685         4657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2066762, whole genome shotgun sequence.
ACCESSION   DASUEG010000685 DASUEG010000000
VERSION     DASUEG010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066762"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VFX11_12920"
     CDS             <1..929
                     /locus_tag="VFX11_12920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939573.1"
                     /translation="LVSMLALGIAIIEWRQGNLLARYFTIAWIFLLTGTCIYTAALAD
                     LVPYNRFTELVQSVGIAAEVILLSLALGVRLNLERQAGRQAIEVSLHLAQQVNLANQE
                     TIHVQERAKAELEEKVQQRTAELANALTQLEQVNATLANLSVTDPLTGLKNRRYFDEQ
                     FAAEFQRALREQSPLSLMVLDLDHFKQVNDRYGHPAGDECLRQTAAVVMAQCRRPGDM
                     AVRLGGEEMLLLLPGTDTDGARAIAETVRARIEALEIQQGRHRFGITTSVGLATVVPA
                     VDDTPEQLLALADKALYRAKGEGRNRVAVAMR"
     gene            977..1954
                     /locus_tag="VFX11_12925"
     CDS             977..1954
                     /locus_tag="VFX11_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939574.1"
                     /translation="MLVPQYWAEARVQQKVGGRQRTMRRFGWSDVSQQQAQQLADERA
                     QAAMSRLQAGDTLPWREPKVPYNGAEGVPIREEVISRHGAVVITRNAYGALCLNTGNV
                     LFADLDFETGVSARLWLAVFCCWFAAALVVATGWQAWGTAFLLVFAGVLLSYPLALLV
                     LRIMSALSGGAEQRARARIHAFSKANPDWHLRVYRTPAGMRVLALQRTFNPLEADVAR
                     CFAALHADPLYRKMCLNQQCFRARVSPKPWRIGVNRHLRPRPGVWPVAPEQLPARQEW
                     VDHYNRLATGFAACRFVERLGSDVSDPEALAVQRLHDELCRAQTELPLA"
     gene            complement(2019..3074)
                     /gene="cobD"
                     /locus_tag="VFX11_12930"
     CDS             complement(2019..3074)
                     /gene="cobD"
                     /locus_tag="VFX11_12930"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX4939575.1"
                     /translation="MTTSGTPSLSPLQHGGNLHAAIARYNIPRADWLDISTGISPFPW
                     PVPTVPPDVWQRLPEQDGALEQAARDYYGADALAVPGSQWAIQQLPALFSRRQGTTRV
                     WLPQECYEEYRYWWQFHGHALQYYRALPARDELQERDIVIVLNPNNPSAHYHAVTELQ
                     TLAQQLQARNGFLILDEAFMDATPAQSLLPHLDADTTSSSDTNVILLRSLGKFFGLAG
                     IRTGFVCGNRKVLEELRHVLGPWAISHPAQWIAIQALQDEHWQHAMRQRLPVQRLRMT
                     NLLARHFPADCISTTPLFITLTLTPERVAHWQDQLARHGIWTRCFMQWGKLRFGLADD
                     AGLARLARALEGCVAND"
     gene            complement(3071..4015)
                     /gene="cbiB"
                     /locus_tag="VFX11_12935"
     CDS             complement(3071..4015)
                     /gene="cbiB"
                     /locus_tag="VFX11_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX4939576.1"
                     /translation="MDFPLSFILILLTKILLALALDQWLGEPRRWHPLVGFGRLAQTL
                     EKALNQGGDWQRFCSGTLAWLLAVLPLTALATALALSPAGFFWDVVLLYLVIGRKSLF
                     QHSRRVWQALQRQDLESARHHTGMMVSRETENLDATGCTRATIESVLENANDGIIGAL
                     FWFALLGAPGAVFFRLANTLDAMWGYRNERFLLFGRAAARIDDVLNFIPARLCALAYA
                     SAGHAVLAIQAWKTQAHLLKSPNGGPVMCAGAGALDLQLGGPAIYHGTRMDKPFFGGH
                     HTPQTHDILRANRLVDRALLILLLFFFLTGAVIAGIAA"
     gene            4091..>4657
                     /locus_tag="VFX11_12940"
     CDS             4091..>4657
                     /locus_tag="VFX11_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX4939577.1"
                     /translation="MTATTLMVQGTTSDAGKSTLVAALCRIFRREGVRVVPFKPQNMA
                     LNSAVTVDGGEIGRAQALQAQAAGLEPVVDMNPVLLKPTTDIGAQVIIHGKARHQMNA
                     VQYHEYKTLAMTAVLESYHRLQQQYELIVVEGAGSPAEINLRDRDIANMGFAEAVDCP
                     VILIADIDRGGVFAHLVGTLDLLSASEQA"
BASE COUNT          886 a         1326 c         1574 g          871 t
ORIGIN      
        1 tgcttgtgtc gatgctggcg ctgggcattg ccatcattga atggcggcag ggcaatctgc
       61 tggcacgtta cttcacgatt gcgtggattt ttttgctgac gggaacctgc atctatacgg
      121 ccgcgctggc ggatctggtt ccctataatc ggtttaccga gctggtacag tcggtgggaa
      181 ttgcggcaga ggtgatactg ctgtcgctgg cgctgggtgt gcgcctcaat ctggaacggc
      241 aggctggtcg gcaggccatc gaagtgtcgc tgcatctggc gcagcaggtg aatctggcca
      301 accaggaaac gattcacgtg caggagcggg caaaagcgga gctggaagaa aaagtgcagc
      361 aacgcacggc ggaactcgcc aatgccctga cgcagctgga gcaggtgaat gcgacgctgg
      421 ctaacctgag cgtcaccgat ccgcttacgg gcctgaagaa ccggcgttat ttcgacgagc
      481 agttcgcagc ggagttccag cgtgcgttgc gggagcagag tccgctgtcg ctgatggtgc
      541 tggatctgga tcatttcaag caggtgaacg accgctacgg ccatccggca ggggatgaat
      601 gtctgcgcca gaccgctgca gtggtgatgg cgcagtgtcg tcgtcctggc gatatggcgg
      661 tgcggctggg cggcgaggag atgttgctgc tgttgccggg caccgacacc gatggcgcgc
      721 gggcgattgc ggaaaccgtg cgtgcgcgca ttgaggcgct ggagatccag cagggccgac
      781 accggttcgg tattaccacc agcgtcggtc tggcaacggt ggtgcccgct gtggacgaca
      841 caccggaaca gttgctggcg ctggcagaca aggcgctgta ccgggcgaaa ggggaaggcc
      901 gtaatcgggt ggccgtggcc atgcgttgaa ggctgtctgt ggcagacatc acttccatca
      961 attcaggagc aggatcatgt tggtaccgca gtattgggcc gaagcgcggg tgcagcaaaa
     1021 ggtcggtggc cgtcagcgta cgatgcgccg gttcggctgg tccgatgtca gtcagcagca
     1081 ggcccagcag ctggctgacg agcgtgcgca ggcggcgatg tcgcgcttgc aggcaggcga
     1141 tacgctgccc tggcgcgaac ccaaggttcc ctacaacggt gcggaaggcg tgccgattcg
     1201 ggaggaagtg atcagccgcc atggtgcagt ggtgatcacg cgcaacgcct atggcgcctt
     1261 gtgcctgaat acgggcaacg tgctgtttgc cgatctggat tttgaaactg gcgtgtcggc
     1321 gcgcctttgg ctggccgtgt tctgctgctg gtttgcggcg gcgctggtgg tggcgacggg
     1381 gtggcaggcg tggggaacgg ccttcctgtt ggtatttgcc ggcgtgctgc tgagctatcc
     1441 tctggcgctg ctggtcttgc gcatcatgtc ggccctgagc ggcggcgccg aacagcgtgc
     1501 ccgtgcccgc attcacgcat tcagcaaggc gaatccggat tggcacttgc gggtctatcg
     1561 cacaccagcg gggatgcgcg tgctcgcgct gcagcgcact ttcaatccac tggaagcgga
     1621 cgttgcgcgc tgcttcgcgg cgctgcatgc cgatccgctg tatcgaaaaa tgtgtttgaa
     1681 ccagcagtgt tttcgcgccc gcgtcagccc caagccctgg cgcattggcg tgaatcgcca
     1741 tctgcgtccg cgacccggcg tgtggccggt agcgccggaa caactgccgg cccgtcagga
     1801 atgggtggat cattacaacc ggctggcaac gggatttgcc gcctgccgtt ttgtcgagcg
     1861 gctgggcagt gacgtgagcg atcccgaagc gctggcggtg cagcggttgc acgatgaact
     1921 ctgccgggcg cagacggaat tgccgctggc gtgagtgatg caattgcgta acttgacgtg
     1981 ggatgccgag ttgccgtcag cggtcgaatt gacgggaact aatcattggc gacgcaacct
     2041 tccagtgccc gcgccagccg cgccagtccc gcatcatccg ccagtccgaa acgcaatttt
     2101 ccccattgca taaaacagcg ggtccagatg ccgtggcgtg ccagctggtc ctgccagtgc
     2161 gcgacgcgtt ccggcgtcag tgtcaaggtg ataaacaatg gcgttgtgct gatgcagtcg
     2221 gcgggaaagt gccgcgccag caaatttgtc atgcgcaggc gttgtacagg cagccgttgc
     2281 cgcatggcgt gctgccagtg ctcatcctgc aatgcctgga tcgcaatcca ttgtgccgga
     2341 tgactgatgg cccagggccc cagcacgtgc ctgagttctt ccagcacttt tctgttgcca
     2401 catacgaagc cggtacggat gccggccagc ccaaaaaatt ttcccagcga acgcagcagg
     2461 atgacattgg tgtcggaact ggaagtggtg tcggcgtcga ggtgcggcag cagcgattgt
     2521 gccggcgttg cgtccatgaa ggcttcatcc agaatcagaa agccattgcg cgcctgcagc
     2581 tgttgcgcca gcgtctgcaa ttccgtcact gcgtggtagt gcgcggacgg attgttggga
     2641 ttgagcacga tgacgatatc ccgttcctgc aattcatccc gtgccggcag cgctctgtag
     2701 tactgcagag catgaccgtg gaactgccac cagtagcggt attcctcata acattcctgc
     2761 ggcagccaga cgcgggtggt gccctgtcga cgggaaaaca gtgcaggtaa ttgctggatc
     2821 gcccactggc tgccaggcac ggccagcgca tcggcgccgt agtaatcgcg agcggcctgt
     2881 tccagtgcgc cgtcctgttc cggcaggcgt tgccacacgt cgggtggaac cgtgggcacc
     2941 ggccagggga acggtgaaat gccagtggaa atatccagcc agtcggcacg gggaatgttg
     3001 tagcgggcga tggcagcgtg cagattgcca ccgtgctgta agggtgaaag tgaaggcgtg
     3061 ccggacgttg tcatgctgcg atgcctgcga tgactgcgcc ggtcaggaaa aagaacagca
     3121 gcaggatcag gagggcgcgg tccaccaggc gattggcgcg caggatgtcg tgggtctgcg
     3181 gtgtgtggtg gccgccgaaa aaaggtttgt ccatccgtgt gccgtgatag atcgccgggc
     3241 cgcccaactg cagatccagc gcaccggcgc cggcacacat caccggaccg ccattgggac
     3301 ttttcagcag atgggcctgg gttttccacg cctgaatcgc caggacagca tggccggcac
     3361 tggcataggc cagcgcgcac aggcgggcgg gaatgaaatt cagtacgtcg tcaatgcggg
     3421 cagcggcacg gccgaacaat agaaaacgct cgttgcggta gccccacatg gcgtccagcg
     3481 tgttggcgag gcgaaaaaag acggcacccg gtgcccccag cagggcaaac cagaacagcg
     3541 cgccgatgat gccgtcgttg gcgttttcca gcaccgattc aatggtggcg cgggtgcagc
     3601 cggtggcgtc cagattttcg gtttcgcggc tcaccatcat gccggtatga tggcgcgcag
     3661 attccagatc ctgccgctgc aatgcctgcc agacgcggcg gctgtgctgg aacaggcttt
     3721 ttcggccgat caccagatac agcagtacca cgtcccagaa gaacccggcg gggctcagtg
     3781 ccagtgcggt ggccagcgcc gtcagcggca ataccgccag cagccaggcc agtgtgccgc
     3841 tgcagaaccg ttgccagtcg ccaccctgat tgagtgcctt ttccagtgtt tgcgccagac
     3901 ggccgaagcc caccagcgga tgccagcggc ggggctcccc cagccattgg tcgagcgcca
     3961 gggccagcag gattttggtc agcaggatca gtatgaaaga cagcggaaaa tccatgggaa
     4021 tcggtatttg ggaatcaata tctgaacggt cggcttgatc agaccgcgaa aaaaggaagc
     4081 gaagtatatc atgaccgcca caaccctcat ggtgcagggc accacatcgg acgccggcaa
     4141 atccacgctg gtggcggcgc tgtgccggat tttccggcgc gaaggcgtgc gcgtggtgcc
     4201 gttcaagccg cagaacatgg cgctcaacag cgcggtgacg gtggatggcg gcgaaatcgg
     4261 ccgggcccag gccttgcagg cgcaggccgc cggattggag ccggtggtgg atatgaaccc
     4321 cgtgctgctc aagcccacta ccgatatcgg cgcccaggtg atcatccatg gcaaggcccg
     4381 ccaccagatg aatgccgtgc aatatcacga atacaaaacg ctggcgatga cggcggtgct
     4441 ggaaagttac caccgcctgc agcagcaata cgagctgatc gtggtggagg gcgccggcag
     4501 cccggcggaa atcaacctgc gcgaccgcga catcgccaac atgggttttg ccgaagcggt
     4561 ggactgcccg gtgatcctga ttgccgacat cgatcgcggc ggcgtcttcg cgcatctggt
     4621 cggcacgctc gacctgctga gcgcgagcga acaggcg
//
LOCUS       DASUEG010000686         6397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_485859, whole genome shotgun sequence.
ACCESSION   DASUEG010000686 DASUEG010000000
VERSION     DASUEG010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485859"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX11_12945"
     CDS             complement(<1..237)
                     /locus_tag="VFX11_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939578.1"
                     /translation="MSLLSLPTDVTALNTLAPKEAGSLIKPADWNALIGSVQGIGTAL
                     QEYIAQTNTRLTTLENAVTPLTSRVDTLEQDLADL"
     gene            complement(266..2707)
                     /locus_tag="VFX11_12950"
     CDS             complement(266..2707)
                     /locus_tag="VFX11_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939579.1"
                     /translation="MSTYEKLVSHLPGLYQPDAQDDTLFNRLLQEVGGNMDELRRDLT
                     LLMQTHWFKTADKATYDPHFLRGRELAGLPRLNLQNPDDKRAIDDYPYLLDLARLGAL
                     LTIPPWREPVTLRENVEQYRQRLLRIIQIYRNGLGTLSALRAMVAAELPETATLPLPA
                     RQRSFTVEENAPFIGPLHSIQSLGLPQFELGPLMRWELANTSVRSVAPVVFIEGIAAG
                     ADTDATVRPMIERFSPTGVLENEGGLAGIGLGYSGTVAPGSVLKLQPGYGACLAGADG
                     ILLSAARTTQLGIDSWQLIPGAPTGDVQALLQTSDKMLWLACDNAGTQRLWRYDGNAW
                     LQILATETLAPIHCLLQHQHQLLIGSDDGIAALDLFPDVANDFALTAFGNIGAQAVFA
                     IEPRRNDTGTFYAGAANGLHELDSTGNITQTLLADTAIHAIAQSDTRLYFGGDAGVMQ
                     YQLQNQQFHYLSAEFESELDADWLPFAADALPDFTFGLPAVQSLALEPDSTLWIGSAR
                     GLARYRARHEQDLVYRNLLEAFPDLLDGAVTQIRADEHGCLWFTTAHGLLRFDGRDLA
                     RFDFVENRWQQLGQADQIYEGEDDHPRGPWRFQRTLNRWEFFDYRATQWTAFSQPPAL
                     PLQAITHLHFIDRVTAELGTLSGSDFSKTADVATSELTLHCKPDQTRIVTGGIPALPR
                     MPQGDSVWRYLSLEPDDLVDGTDLPWWSVEGRLVPPPQHNPPYAGRFHHLDLLPYQLD
                     RMVFSYNPAARIKFQWANQKPLRVIVRLQQRNSDDLIDPAILDRVWQGINRVRPAGVQ
                     VALAVGDTLVRGESV"
     gene            complement(2704..4503)
                     /locus_tag="VFX11_12955"
     CDS             complement(2704..4503)
                     /locus_tag="VFX11_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939580.1"
                     /translation="MSFRRRTYPEVMENVLTGLTGGIAAESHAYPPAGAKTAPFAHAL
                     EHPPVDAIISVYGIRNNQTFQFVKGSDYELKQDKLVWLEKANTPDAGTLLHINYRSKG
                     AQSPFNDLHVGSVLRTLAESFSLELAGLYAQAEAVYKSAFIDTAEGRSLDNVVALLDV
                     NRVRAGRNTTKVKFTRSANGVGDIHIPAGTRVTNADASVQFETIAAITLLDGQTSVQV
                     DARDIADNLEALPADSLVILTKPLAGIDSVTNPNPSSVLERDETDDELRTRAKNFLHG
                     SEKATLGAIKHAIAQQNVVAEVSENDPEPGKITVTVHSDNLTPETEQRLLAAIKDARP
                     AGIEVILAGVSAPFVLDLELRISTAADLLEADLRGIQERAVQRIIAYLNGSAIGDTIS
                     LNRIVGLVLSDNTVLDVRILSARVGSDDVLDRDQGIIKTDNISGVSPGSKVALRAGAI
                     NIIDPALATVLSASLRFPVSAAAPSESAIRAALASAIEAINLLNASELPASPTPTQLR
                     QRQLKYGKLLAVIPLPGKPAVTSYNDVAGLDDASLPTSSAAGDYQVQFIVTSESGISH
                     LLEDETSPAVTLIPFERLTLANVELKAQAEVTA"
     gene            complement(4529..4987)
                     /locus_tag="VFX11_12960"
     CDS             complement(4529..4987)
                     /locus_tag="VFX11_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX4939581.1"
                     /translation="MSSDAHLLTDLALDLGDHRIRPLYKVSDEERRLPAGRGGVLDIR
                     LLSGRDNLQQAIVMRLLTPVGELAGLGHPDYGSRLFDLVGRVNTDTTRNLVRLYILES
                     LQREPRIGKINRIDVRQVERQPSLVAVELDVTPIASTDVISIGPFTLSLI"
     gene            complement(5020..5412)
                     /locus_tag="VFX11_12965"
     CDS             complement(5020..5412)
                     /locus_tag="VFX11_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX4939582.1"
                     /translation="MGQPAAKKGDQIVGTDTHIVMIPSPGGPVPTPLPHPFTGQIDDA
                     LSSDVNIDGQPAAVVGSKASNMPSHIPQGGPFQTPPKDKAEIQMGSSSVFINGKAAAR
                     NGDTALTCNDPADAPVGTVIAVGTVNIG"
     gene            complement(5461..6090)
                     /locus_tag="VFX11_12970"
     CDS             complement(5461..6090)
                     /locus_tag="VFX11_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX4939583.1"
                     /translation="MTTSLYDTIRSIVRQELRQLRLGELAVVQEQHPHESDSDKDNYS
                     CTVVLRDSAIVLRQVPVATGRLGVASIPDVGDLVLVQFLNGDINAPIIVASLYNDEDR
                     PPLNKDGEVILQLPKSGSEGEGVHVRASSVDETSITLNLGGTVVVTLKDDDPAVEINI
                     GDGSGVIKIDQDGTVNITSQAELKLKSDADINIEAGGTLNLKGSTINLN"
     gene            complement(6087..>6397)
                     /locus_tag="VFX11_12975"
     CDS             complement(6087..>6397)
                     /locus_tag="VFX11_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939584.1"
                     /translation="PSLRSSDAVQQAAAGKLFFARQRALKVRLLVLGAPNIRAGSKIE
                     IADAPQPEWNGTAVVESVRHQYSKLKGFTSDITALLESADNSMDSLLAGALGALGGLL
                     "
BASE COUNT         1034 a         1910 c         2106 g         1347 t
ORIGIN      
        1 cagatccgcc agatcctgtt ccagtgtatc gacgcgggag gtcagcggcg tcaccgcatt
       61 ttccagcgtg gtgaggcggg tgttggtctg cgcgatatat tcctgcaagg cggtgccgat
      121 gccttgcacg ctgccgatca atgcattcca gtccgccggt ttgatcagcg agccggcttc
      181 cttgggcgcc agtgtgttca gcgccgtcac gtcggtgggg agtgagagta agctcatgtc
      241 cgttctcctc taaatggatt cgccgctaga ccgattcgcc gcgcacgagg gtgtcgccca
      301 ccgccagcgc cacttgcacg ccggcgggac gcacccggtt gatgccctgc cagacgcgat
      361 ccagaatggc cggatcgatc aggtcgtcgc tgttgcgttg ttgcagccgc acgattacgc
      421 gtagcggctt ttgattcgcc cactggaatt ttatgcgcgc cgccgggttg taggaaaaca
      481 ccatccggtc cagctggtag ggcagcaggt ccagatgatg gaagcggccc gcatagggtg
      541 gattgtgttg tggcggtggc accagccggc cttccaccga ccaccagggc agatcggtac
      601 cgtccacgag gtcgtccggt tccagcgaca gataacgcca cacggaatcg ccctggggca
      661 tgcgtggcag cgcgggaatg ccgccggtga cgatgcgggt ctggtccggt ttgcagtgca
      721 gtgtgagttc gctggtggcg acatcggcgg ttttgctgaa gtcgctgccg ctgagggtgc
      781 ccagctcagc cgtcacgcga tcgataaaat gcagatgcgt gatggcttgc aggggcagtg
      841 ccggcggttg ggaaaaggcc gtccactggg tggcgcggta atcgaaaaat tcccagcgat
      901 tcagcgtgcg ctgaaaacgc caggggccac ggggatggtc gtcttcgcct tcgtaaatct
      961 ggtcggcctg gcccagttgc tgccagcggt tttccacaaa atcgaaacgc gccagatcgc
     1021 ggccgtcgaa acgcagcagg ccgtgagcag tggtgaacca gaggcagcca tgttcgtcgg
     1081 cgcgaatctg cgtgacggcg ccatccagca gatcgggaaa ggcttccagc agattgcggt
     1141 acaccagatc ctgttcgtgg cgggcgcggt agcgggcgag accgcgcgcg ctgccgatcc
     1201 acaaggtgct gtccggttcc agcgccagtg actgcacggc gggcaggccg aaggtgaaat
     1261 ccggcagggc gtcggcggcg aagggcagcc agtcggcgtc cagttccgat tcgaattccg
     1321 cgctcaggta atggaactgc tggttttgca attgatactg catcacgccg gcgtcgccgc
     1381 caaaataaag ccgggtgtcg ctttgcgcga tggcgtgaat ggcggtgtcg gccaagagtg
     1441 tctgcgtgat gttgccggtg ctgtcgagtt catgcaggcc gttggcggca ccggcgtaaa
     1501 atgtaccggt gtcgttgcgg cgcggttcga tggcgaacac cgcctgtgcg ccgatattgc
     1561 cgaacgcggt gagcgcgaaa tcattggcga cgtcaggaaa cagatccagc gcggcaatac
     1621 catcgtcgct gccgatcagc aactggtgtt gatgttgtag caggcagtgg atgggcgcga
     1681 gggtttctgt tgccagaatt tgcaaccagg cgttgccatc gtaacgccag agtcgttgcg
     1741 tgccggcatt gtcgcaggcg agccagagca ttttgtcgct ggtctgcagc agtgcctgca
     1801 cgtcgcccgt tggcgcgccg ggaatgagtt gccagctgtc gatgcccagt tgcgtggtgc
     1861 gtgccgccga caacagaatg ccgtcggcac ccgccagaca ggcgccgtaa cccggctgca
     1921 gtttcagcac gctgcccggc gccacggtgc cggaataacc gagcccgatt cccgccagtc
     1981 cgccttcatt ttccagcacg ccggtggggc tgaagcgttc gatcatgggc cgcacggtgg
     2041 cgtcggtgtc ggcgccggcg gcgatgcctt cgataaagac caccggtgct acgctgcgca
     2101 cgctggtgtt ggccagttcc cagcgcatca gcgggcccag ttcgaattgc ggcaggccca
     2161 ggctttgaat gctgtgcagc ggaccgatga agggcgcgtt ttcctccacc gtgaacgagc
     2221 gttgccgcgc gggcaatggc agcgtggcag tttccggcaa ttccgccgcc accatggcgc
     2281 gcagtgccga cagtgtgccc agtccgttgc ggtaaatctg gatgatgcgc agcagccgct
     2341 gccggtattg ctcaacgttt tcccgcagcg tgaccggttc gcgccagggc ggaatcgtca
     2401 gcagcgcacc cagccgcgcc agatccagca gatagggata gtcgtcgatg gcgcgcttgt
     2461 catccggatt ctgcaggttg aggcggggca aaccggccag ttcgcgcccg cgcagaaaat
     2521 gcggatcgta ggtggccttg tcggcggtct tgaaccagtg cgtctgcatc agcagggtca
     2581 gatcgcggcg cagttcgtcc atgttgccgc ccacttcctg cagcaggcgg ttgaacagcg
     2641 tgtcgtcctg cgcgtccggc tggtagaggc cgggcagatg gctcaccagc ttttcatacg
     2701 tgctcacgcg gtcacctcgg cttgcgcttt caattccacg ttggcgagcg tcaggcgttc
     2761 gaacggaatc agcgtcacgg cgggactggt ttcatcttcc agcagatggc tgatgccgct
     2821 ttcggacgtg acgatgaact gcacctgata atcgccggcg gcgctgctgg tgggcaggct
     2881 ggcatcgtcc agcccggcca cgtcgttgta gctggtgacg gcgggtttac cgggcagcgg
     2941 aatcaccgcc agcagcttgc catacttgag ctggcgttgc cgcagttgcg taggtgtcgg
     3001 gctggcgggc agttcgctgg cgttgagcag gttgatggct tcgatggcgc tggccagtgc
     3061 ggcgcggatg gcgctttccg atggcgccgc cgcactcacc gggaagcgca ggctggcgga
     3121 caagaccgtg gccagtgccg gatcgatgat gttgatggcg ccggcgcgca gcgccacttt
     3181 gctgccgggc gaaacgccgc tgatgttgtc ggtcttgatg atgccctggt cgcggtcaag
     3241 cacatcgtcg ctgccgacgc gggccgacag gatgcgcaca tccagcacgg tgttgtcgct
     3301 cagcaccagg ccgacgatgc ggttgaggct gatggtgtcg ccgatggcag atccattcag
     3361 ataagcgatg atgcgctgca ccgcgcgctc ctggataccg cgcagatccg cttccagcaa
     3421 atccgctgcc gtgctgatgc gcagttccag atccagcacg aacggtgcgc tgacgcccgc
     3481 gagaattacc tcgatgccgg ccgggcgtgc gtctttaatt gcggcgagca ggcgctgttc
     3541 ggtttccggc gtcagattgt cgctgtgcac ggtgacggtg atcttgcccg gctccggatc
     3601 gttctcgctc acttccgcca ccacgttctg ttgtgcgatg gcgtgcttga tggcgccgag
     3661 cgtggccttc tcactgccgt gcagaaaatt cttcgcccgc gtgcgcagtt cgtcgtcggt
     3721 ctcgtcccgt tccagcacgc tgctggggtt gggattggtg acgctgtcga tgccggccag
     3781 cggtttggtg agaatcacca gtgaatccgc cggcagggct tccagattgt cggcgatgtc
     3841 gcgcgcgtcc acctgcacgc tggtctggcc gtccagtagc gtgatggcgg cgatggtttc
     3901 aaactgcacg ctggcgtcgg cattggtgac gcgggtgccc gccggaatgt gaatgtcgcc
     3961 gacgccattg gcgctgcggg tgaatttaac cttggtggtg ttgcggccgg cgcgcacgcg
     4021 gttgacatcc agcagcgcca ccacgttgtc gagggaacgg ccttcggcgg tgtcgatgaa
     4081 ggcggatttg tacaccgctt ccgcctgcgc atacaggccc gccagttcca ggctgaagga
     4141 ttctgccagc gtgcgcagca cactgcccac gtgcagatcg ttgaacggcg actgcgcgcc
     4201 cttgctgcgg tagttgatgt gcagcagcgt gccggcgtcc ggcgtgttgg ctttttccag
     4261 ccacaccagt ttgtcctgct tcaattcata atcgctgcct ttgacgaact ggaaggtctg
     4321 gttgttgcga atgccgtaca cggaaatgat ggcatccacc ggcggatgct cgagggcatg
     4381 ggcaaagggt gcggtcttgg cgccggcggg aggataggcg tggctttccg ccgcgatgcc
     4441 gccggtcagg ccggtcagca cattttccat gacctccggg taggtgcgac gtctgaaact
     4501 catggcattg ggctccgttg ctgcgctttt aaattaatga aagggtgaat gggccgatgc
     4561 tgatcacatc ggtgctggcg atgggcgtca cgtccagttc caccgccacc aggctgggct
     4621 gacgctccac ctggcgcaca tcgatgcggt tgatcttgcc gatgcgcggt tcccgctgca
     4681 ggctttccag aatatacagg cgcaccagat tgcgggtggt gtcggtgttg acgcggccca
     4741 ccagatcaaa caggcgcgaa ccgtaatccg gatggcccag gccggcgagt tcgcccaccg
     4801 gtgtcagcag acgcatcacg atcgcctgct gcaaattgtc gcggccgctg agcagacgga
     4861 tatcgagcac gccaccgcgg ccggcgggca ggcgccgttc ctcatcgctc accttgtaca
     4921 gggggcggat gcggtgatcg cccaggtcca gggccagatc ggtgagaaga tgtgcgtcgc
     4981 tgctcatgcg tgtttttccc ggttcgtctt aataatctgt catccaatat tcacggtgcc
     5041 caccgcgatc accgtgccca ccggggcgtc agccggatcg ttgcaggtga gtgccgtgtc
     5101 gccgttgcgt gctgccgcct tgccgttgat aaacacgctg ctgctgccca tctggatttc
     5161 agccttgtcc ttgggcggtg tctggaacgg cccgccctgc ggaatgtgcg acggcatatt
     5221 gctggccttg ctgcccacca ccgcggcggg ctggccgtcg atgttgacgt cgctgctgag
     5281 cgcgtcgtcg atctggccgg tgaacggatg cggcagtggc gtgggcacgg ggccgcccgg
     5341 tgacggaatc atcacgatgt gggtgtcggt gccgacgatc tggtcgcctt tttttgctgc
     5401 cggttgtccc atgacttttt gcttccctgt tatctgtttg ccctgttcac ttcacgttgt
     5461 tcagttcaga ttgatcgtcg agcctttcag gttcagtgtg ccgccggcct cgatattgat
     5521 gtcggcgtcg cttttcagtt tcagttccgc ctggctggtg atgttcacgg taccgtcctg
     5581 atcgatcttg atgacgccgc tgccatcgcc gatattgatt tccaccgccg gatcgtcgtc
     5641 cttcagtgtt accaccacag tgccgcccag attaagcgtg atgctggttt catccacgct
     5701 gctggcgcgc acgtgcacgc cttcaccctc gctgccggat ttcggcaatt gcagaatcac
     5761 ctcgccgtct ttgttcagcg gcgggcggtc ttcatcgttg tacaggctgg cgacgatgat
     5821 gggcgcgttg atgtcgccat tcagaaactg caccagcacc agatcgccca cgtcgggaat
     5881 ggacgccaca ccgagacgcc cggtggccac cggcacctgc ctgagcacaa tggccgaatc
     5941 ccgcagcacc accgtgcagc tgtaattgtc cttgtcggaa tcggattcgt gcggatgctg
     6001 ctcctgcacc accgccagtt cgcccagccg caactggcgc aattcctggc gcacgatgct
     6061 gcggatggtg tcgtacaggc tggtggtcat agcaatcctc ccagtgcacc cagcgcgcca
     6121 gccagcaggc tgtccatgct gttgtcggcg ctttccagta acgcggtgat gtcgctggtg
     6181 aagcctttca gcttgctgta ctgatggcgc acggattcga ccaccgcagt accgttccat
     6241 tccggctgcg gcgcgtcggc gatttcaatt ttgctgccgg cgcggatgtt gggcgcgccc
     6301 agtaccagca ggcgtacttt cagtgcgcgt tgtctggcga aaaacagttt gcctgccgcc
     6361 gcctgttgca ccgcgtcgct gctgcgcagg ctgggat
//
LOCUS       DASUEG010000687         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_207012, whole genome shotgun sequence.
ACCESSION   DASUEG010000687 DASUEG010000000
VERSION     DASUEG010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207012"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFX11_12980"
     CDS             <1..743
                     /locus_tag="VFX11_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4939585.1"
                     /translation="DPSLLFRYFDGLFLYGFFYAWSKTETLAAFESHFLASAPRDFNW
                     YVTMWRRNLVSEKQDRVVAKLFSLSLRLPVFLQRFPDARILYMVRDPLETVPSGLSLV
                     TGVLDGRFGFWRLPEAQRRHYIENMYAALLALSLRFHADYTSGRIPACNLYIVTFPRL
                     MREFDTVMGEIMHFVGMESTPELETTIAHIAEKQRRYKSGHEYDLARFGLTEERIRQD
                     YAVIYDTFLQDPSATRRTTAAMKVTLPV"
     gene            complement(709..1953)
                     /locus_tag="VFX11_12985"
     CDS             complement(709..1953)
                     /locus_tag="VFX11_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEX4939586.1"
                     /translation="MDVLPRIRQRLACHRSLLLAISLLCGLTACDQYDVKIYKPDKVW
                     DGYTVPSSALGGNTPVIDMDGNRIMRFKCAGFPAKLLPDGSMVCHNLKTGALEIPELR
                     QVAPDGQVLWRFRDWSRNSAKQHHDFQFSGGNPVGYYVPGMDMIGPADGGNMLVLSHY
                     VNARSAQDIRKGSLLDDVLYEIDAAGNILWQWHAKDHIDEMGFSESALQDLYIEPLMR
                     DWLHTNTASYIGPNKWYDEFNDQRFHPDNIIIASPRARWIAIIDKPTGNLVWRMGPDY
                     SPGNPGAEFGGVNFSHHAHIIPKGLPGEGNLLVFDNGTFSGFGDQYSPGRLYSRVLEI
                     NPIDFSVVWEYRGNLFSLNMSSAQRLPNGNTFITEGLKGRMLEVTADKEVVWEARGLP
                     LYRAYRVPKEWVKPATLLSSPQ"
     gene            complement(2093..2722)
                     /gene="cysC"
                     /locus_tag="VFX11_12990"
     CDS             complement(2093..2722)
                     /gene="cysC"
                     /locus_tag="VFX11_12990"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX4939587.1"
                     /translation="MTSPYETKPHQSAASVVWHHTRCNAEDRARLKQQKPVCVWLTGL
                     SASGKSTLANALEACLLEQGRHTYLLDGDNVRHGLNRDLGMSEADRAENIRRIGEVAR
                     LMTDAGLIVITAFISPFRADRDQARTLFGKEQFIEVFIDTPLTVCEERDPKGMYKKAR
                     AGQITGFTGIDSPYEAPLAPELRVDTAQKSVAQCTHEIFTFLQPRILPW"
BASE COUNT          552 a          745 c          784 g          655 t
ORIGIN      
        1 acgatccgtc gctgctgttc cgctattttg acggcctgtt tctgtacgga tttttttatg
       61 cctggtcgaa aaccgagaca ctggctgcgt ttgaatcaca ctttctggcc agcgcgccgc
      121 gggatttcaa ctggtacgtc acgatgtggc gccgcaatct ggtgagcgag aagcaggatc
      181 gggtagtcgc caaacttttt tcactgtcgc tgcggttgcc cgtgtttctg caacgatttc
      241 cggatgcccg catcctgtat atggtgcgcg atccgctgga gacggttcct tccggtctca
      301 gcctggtaac cggtgtgctc gatggccggt ttggcttctg gaggctgcca gaggcgcaac
      361 gccggcacta tatcgaaaac atgtatgccg cgttgctggc gctgagcctg cgctttcatg
      421 cggattacac cagcggcaga attcccgcct gcaatcttta tatcgtcacg tttccccggc
      481 tgatgcgcga gttcgacacc gtgatgggcg aaatcatgca tttcgtcggc atggaatcga
      541 cgccggaact ggaaaccacg atcgcgcata ttgccgaaaa gcagcgccgc tacaaaagcg
      601 ggcatgaata tgatcttgcg cgtttcgggc ttacggaaga acggatccgg caggattacg
      661 ctgtcatcta tgacaccttc ctgcaggatc cgtctgctac ccggcgaact actgcggcga
      721 tgaaagtaac gttgccggtt tgacccattc tttcggcacg cggtaggcgc ggtacagggg
      781 cagtccccgc gcctcccaca ccacctcctt gtccgccgtg acttccagca tgcgtccctt
      841 caagccttcg gtgatgaacg tgtttccgtt gggtagccgt tgggccgaac tcatgttgag
      901 gctgaacagg ttgccgcgat attcccacac cacagaaaaa tcgatggggt tgatttccag
      961 tacgcgggaa tagagtctgc ctggcgaata ctggtcgccg aatcccgaga acgtaccgtt
     1021 atcgaacacc agcagattac cttcccccgg aagccctttg ggaatgatgt gggcatgatg
     1081 cgagaaattc acgccgccaa attctgcacc gggattgcct ggtgagtagt caggccccat
     1141 ccgccacacc agatttccgg ttggtttgtc gatgatggcg atccagcggg cgcggggtga
     1201 ggcgatgatg atgttgtcag gatggaagcg ctggtcgttg aattcgtcat accacttgtt
     1261 gggcccgatg tacgaggcgg tgttggtatg cagccagtcc cgcatcaggg gttcgatata
     1321 aagatcctgc agcgcggatt cgctgaaccc catttcgtca atgtgatcct tcgcgtgcca
     1381 ttgccacagg atgttgcctg cagcatcgat ttcgtacagc acatcgtcga gcaggctgcc
     1441 cttgcggata tcctgcgccg atctggcgtt gacgtagtga ctgagcacca gcatgttgcc
     1501 gccatccgcc ggcccgatca tgtccatgcc tggcacataa tagccaaccg gattgccgcc
     1561 actgaactgg aaatcgtggt gctgtttggc cgagttgcgg ctccagtcgc gaaagcgcca
     1621 cagcacctgg ccatccggcg ccacctgtcg cagttcggga atttccagtg cgccggtttt
     1681 caggttgtgg cacaccatgg agccgtcggg cagcagtttg gcaggaaatc ctgcgcactt
     1741 gaatcgcatg atccgattgc cgtccatgtc gatgaccggc gtgttgccgc ccagcgcact
     1801 ggatggaacg gtatagccat cccacacctt gtctggttta taaatcttga cgtcgtattg
     1861 gtcgcatgcc gtgaggccgc acaaaaggga aattgcaagc aggagtgaac gatgacaggc
     1921 aagccgttgc ctgatgcggg gcaaaacatc catgttattt cttcctgaag agttattttt
     1981 tttactgcag gtcgagcata tttttttcga cggaaaattg taaagaacag aaatgtagtc
     2041 gtattgttta cgacaagtgc gtagctgaaa tttctatttt tcctgatagg ttctaccagg
     2101 gcaggatgcg aggctgcagg aaggtaaaaa tttcatgcgt acattgcgcc actgactttt
     2161 gggcggtatc gacacgcagt tcaggagcaa ggggcgcttc atagggtgaa tctatgccag
     2221 tgaaacccgt gatctggcca gccctggcct ttttatacat gcctttggga tcgcgctcct
     2281 cacaaacggt gagcggcgtg tcaataaata cttcgataaa ctgctctttg ccgaacaggg
     2341 tccgggcctg gtcgcggtcg gcacgaaagg gcgaaataaa tgcagttatc acaatcagtc
     2401 cggcatccgt catcaggcgc gccacttcgc caatgcgacg gatattctcg gcgcggtctg
     2461 cttccgacat gcccagatcc cggttgaggc catggcgcac gttgtcgcca tcgagcaggt
     2521 aggtgtgccg cccctgttcc agcaggcagg cttccagtgc attggccaga gtggattttc
     2581 ccgatgccga caatccggtc agccacacgc acacgggttt ctgctgtttc agtcgtgcgc
     2641 gatcctcggc gttgcaacgg gtgtggtgcc agaccacgct ggcggcgctc tgatgcggtt
     2701 tggtttcgta aggcgatgtc atggctaaag tccttc
//
LOCUS       DASUEG010000688         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1686558, whole genome shotgun sequence.
ACCESSION   DASUEG010000688 DASUEG010000000
VERSION     DASUEG010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686558"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFX11_12995"
     CDS             complement(<1..440)
                     /locus_tag="VFX11_12995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4939588.1"
                     /translation="MTQDTPSSPDSVAALAWDRPHPFILPIQVTSAHIDVMGHTNNVV
                     YLEWLEKVAWAHSNSLGLDWDVYRKLNRALVARRHEIDYLAATFAGDALLLGTWIVDN
                     DRKLSLTRRYQIIRPADGVTVLRGQTHWVCIELDSGKPRRMPPE"
     gene            complement(514..1224)
                     /gene="tsaB"
                     /locus_tag="VFX11_13000"
     CDS             complement(514..1224)
                     /gene="tsaB"
                     /locus_tag="VFX11_13000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4939589.1"
                     /translation="MPPTLLAIDTSSEACSVALQTGDQLLFRFTDQPRKHAELILPMV
                     DEVLAEAGLRLQQLDALCFGRGPGSFTGLRIAAGMVQGLAFGADLPVVPVSSLAGLAQ
                     RAVREFGWTRIHAAFDARMGEVYWGSYRVNDAGDLELVGRECVCVPAQLQQAAGIDAQ
                     AGWYGAGSGWVFRDILETATGALQQCEPTLLPHARDLLPLATLYWQRGESISAEQVVP
                     VYLRNDVAVKSAPRPAKG"
     gene            complement(1282..>2134)
                     /locus_tag="VFX11_13005"
     CDS             complement(1282..>2134)
                     /locus_tag="VFX11_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX4939590.1"
                     /translation="EEAVLQADTGGRWLRLIAGRVQSPFASIAATRANWIFISATLAV
                     DNSFAWFKRILDLPQLQVQRFAGELDYARQAMLWIPESLPEPADEAFMPAWIEQVLAV
                     AQDLDGGMLLLFSSHEALQNAAQLLQGRTDRQLLVHLPGSHRQQLLQQFRQNDQSLLL
                     GTGSFWEGIDVSGAALRCIAIDKLPFTPPDDILALAWKFMAAKQGQHLFNDYMVPQAI
                     TRFRQGIGRLLRSSDDRGLILVGDTRLLRKPYGQRFLSSLPPVPVATSFAAVQAFFHE
                     QGLLRPD"
BASE COUNT          454 a          701 c          618 g          361 t
ORIGIN      
        1 aattccggtg gcatccggcg cggtttgccg gagtccagtt cgatacagac ccagtgggtc
       61 tgaccccgca acacggtcac gccatccgca ggccggataa tctgatagcg acgggtcagc
      121 gacagcttgc ggtcattgtc cacgatccag gtgcccagca acaatgcgtc ccccgcgaac
      181 gtcgcggcaa gataatcgat ttcatgacgc cgtgccacca gcgcccggtt caatttgcga
      241 tagacatccc aatccagccc cagcgagttg gaatgggccc aggccacctt ttccagccac
      301 tccagataga cgacattatt ggtgtggccc atgacatcga tatgcgcgga cgtgacctgg
      361 atgggcagta taaaaggatg cggacggtcc caggcgagtg ctgcaacgga atccggcgat
      421 gatggtgtgt cctgtgtcat ggaaggttcc ccgtgaaaat ccgtggcggc gcaatcgtgt
      481 gcgtcgtcgg taaatgcacg acagacgtcg ctgtcagcct ttcgccggcc gcggtgccga
      541 cttcaccgcc acatcattgc gcagatacac cggcaccacc tgttcggcac tgatgctttc
      601 gccccgctgc caatacagcg tggccagcgg cagcagatcg cgggcatggg gcagcagcgt
      661 cggttcacac tgctgcaagg cgcctgtggc ggtttccagt atgtcgcgaa acacccagcc
      721 gctgccggcg ccataccagc ctgcttgcgc atcaataccg gcggcctgct gcaactgcgc
      781 cggcacgcac acacactcgc gccccaccag ctccagatcg cccgcatcat ttacccgata
      841 actaccccag tacacttcgc ccatgcgtgc gtcgaacgcg gcatgaatac gggtccagcc
      901 gaattcacgc actgcccgct gcgccagccc cgccagcgac gacaccggta ccaccggcag
      961 atcggcgccg aaggccagcc cctgcaccat acccgccgca atgcgcagcc cggtaaatga
     1021 accggggcca cggccaaaac agagcgcatc cagctgctgc aggcgcagac ccgcttccgc
     1081 cagcacctca tccaccatcg gcagaatcag ttccgcatgc ttgcgcggct gatcggtaaa
     1141 acggaacagc agctgatcgc cggtttgcag ggcgacagaa caggcttcag acgatgtgtc
     1201 gatggcaagc agtgtaggcg gcatcggagc tcccgggttg atgcgcgcat tatcgcagga
     1261 atgggcggcg ggacaaacgc gtcaatcggg tcgcaataat ccctgctcgt gaaaaaatgc
     1321 ctgcacggca gcgaaggatg ttgccacagg caccggcggc agactggaca gaaagcgctg
     1381 tccataaggc ttgcgcagca gacgggtgtc acccaccagg atcaggccac ggtcgtcgct
     1441 ggaccgcagc agtcgaccaa tcccctgccg gaagcgggtg atcgcctgcg gcaccatgta
     1501 atcgttgaac agatgctggc cctgctttgc cgccatgaat ttccacgcca gcgccagaat
     1561 gtcgtcaggt ggggtgaacg gcagcttgtc gatggcaata cagcgcaacg cagcaccgct
     1621 gacatcgatg ccttcccaga aactgccggt acccagcagc aggctctggt cgttctggcg
     1681 gaattgctgc aacagctgct gacgatgact gcccggcaaa tggaccagca attgacggtc
     1741 ggtgcgcccc tgcaacagct gtgcggcgtt ctgcagcgcc tcatgactgg agaacaacag
     1801 cagcatgcct ccatccagat cctgcgctac cgccagcacc tgctcgatcc aggccggcat
     1861 gaacgcctcg tccgccggct ctggcaggga ttccggaatc cacagcattg cctgccgcgc
     1921 ataatccagt tcgccagcaa aacgttgcac ctgcaattgc ggcagatcca gaatgcgttt
     1981 gaaccaggcg aacgaattgt ccaccgccag tgtggccgaa atgaaaatcc agttggcgcg
     2041 ggtcgccgca atgctggcaa agggcgattg cacgcgtccg gcgatcaatc gcagccagcg
     2101 accgccggtg tcagcctgca gcactgcctc ctcc
//
LOCUS       DASUEG010000689         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_334384, whole genome shotgun sequence.
ACCESSION   DASUEG010000689 DASUEG010000000
VERSION     DASUEG010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334384"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX11_13010"
     CDS             <1..263
                     /locus_tag="VFX11_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX4939591.1"
                     /translation="ANAVIRAQKAAGLPCDGNNVFLNDGPAANQHVPHVHLHVLPRKL
                     GDLHRAVLSFSTRYLNYFGQEAHRNRLDELAQRIARQMPQQV"
     gene            267..1076
                     /locus_tag="VFX11_13015"
     CDS             267..1076
                     /locus_tag="VFX11_13015"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX4939592.1"
                     /translation="MCELMGMSANVPTDICFSFAGLIRRGGETGPHKDGWGITFFEGK
                     GVREFKDVDASATSEIARFVKDYPIKSHVVISHIRKANSGRVALENTHPFTREMWGLP
                     WVYAHNGQLRGVKKLPLTHYRPVGTTDSEFAFCYILNRLRDRFGDAPPRKSALFRAIH
                     ALCEELRQYGVFNILFSDSKFLYCYCTTKLCRITRRSPFGVAHLRDKEVTVDFSDMAV
                     ANDVVTVIATAPLTTNEPWEYMQTGELLVFQRGDVACRLLPKPVRSLSSHG"
     gene            1069..>3000
                     /locus_tag="VFX11_13020"
     CDS             1069..>3000
                     /locus_tag="VFX11_13020"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX4939593.1"
                     /translation="MADDGSKRDDSATPRIGARILVELLRLHAEEPATDKIKLVPTRS
                     RNAMAVLHDKLWQVQQTLQDAGLKLNAANALAERCSELQIPLPVAQPDWGLILLLDQL
                     FDQLLAGDGLDAQAVDWLASLRLPVIRYALQDYSFFFAPQNLVRRFLNQLHLNLLSSS
                     HKSRQILRDALAHFIRRIQQDFRQDVSQFNSVCIEAQSWFAGQDDKVGQIEQQLRSLE
                     SVRRKERVAEPRVVQELNRIAAGKALPRQLAEFLHGEWRKSLRLVSMREGEEGTTWKR
                     QVRITESLVEFALECVDAKGRDKHRSFYPVMMKNIRSLLVSVSDDPAALEAALDPLEL
                     VLMALLSGANLEAVKVPALQLTSAQVTMAEVKQVSPRALAEIQALQTGAWLRVKTTDG
                     SHELCRLTIKSSDDEPWVLVSQSGKTIIKKTAFQLAQGLEGGVLERIGQTHWWDEKLD
                     TVLADLHVRWQRWQTEQPAAPAPQPESRIDILLNTPSTPPAPKAPVVAAPAIKVPEVT
                     AESPPAVAEVAAASSQTPDAASVPVAETGGVDAVDFLAPRPVSEAERNAALQAIDQLQ
                     VGGWILQQTSKGEERCKLAVRIRGREKLVFVNRLGVKVLEIDRVPLAELLARGLVTII
                     DTGVKFNNTLERVVRSIQEE"
BASE COUNT          597 a          893 c          953 g          557 t
ORIGIN      
        1 tggccaatgc ggtcatccgc gcgcaaaaag cggcaggcct gccttgcgac ggcaacaacg
       61 tcttcctgaa tgacggaccc gccgccaacc agcacgtgcc gcatgttcac ctgcacgtgc
      121 tgccgcgcaa gctgggcgat ctgcaccgtg ccgtgttgtc cttttccacc cgctatctga
      181 attatttcgg ccaggaggcc caccgcaacc ggctggacga actggcccag cgcattgccc
      241 gccaaatgcc gcagcaggtg tagcccatgt gtgaattgat gggcatgagt gccaatgttc
      301 ccaccgatat ctgcttcagt tttgcggggc tgatccggcg cggcggtgaa accggcccgc
      361 acaaggatgg ctggggcatt acctttttcg aaggcaaggg cgtgcgcgaa ttcaaggacg
      421 tggatgccag cgccacctcg gaaatcgcgc gttttgtgaa ggattatccg atcaagagcc
      481 atgtcgtcat cagccatatc cgcaaggcca acagtggccg ggtggcgctg gaaaacacgc
      541 accctttcac ccgggaaatg tggggcctgc cctgggtgta tgcacacaac ggccagttgc
      601 ggggggtgaa aaaactgccg ctcacgcatt accgccctgt gggcaccact gacagcgaat
      661 tcgccttctg ctacattctc aaccgtttgc gggaccgctt cggcgatgct cctccccgca
      721 agagcgcact gttccgcgcg atccatgcct tgtgcgagga attgcgccaa tatggcgtgt
      781 tcaatatcct gttcagcgat tcgaagttcc tgtattgcta ttgcaccacc aaactgtgtc
      841 gcattacccg ccgcagcccg ttcggcgttg cccatttgcg tgacaaggag gtcaccgtgg
      901 atttcagcga catggcagtt gccaatgatg tggtgaccgt gatcgccacc gcgccactga
      961 ccaccaatga accctgggaa tatatgcaaa ccggtgagct gctggtgttt cagcgtggcg
     1021 acgtggcctg ccggttgttg ccaaagcctg tccggagcct ttcaagccat ggctgacgac
     1081 ggcagcaagc gtgacgacag cgccacaccg cgcatcggtg cccgcattct ggtggaactg
     1141 ttgcgcctgc acgcagagga gccggccacc gacaagatca agctggtacc gacgcgcagc
     1201 aggaacgcga tggcggtgct gcatgacaaa ctctggcagg tgcagcaaac cctgcaggat
     1261 gccggactga aactcaatgc agccaacgca ctggcagagc gttgcagtga gctgcagatt
     1321 ccgctgccgg tggcgcagcc cgactggggc ctgatcctgc tgctggacca gctgttcgat
     1381 caactgctgg cgggcgatgg cctggatgca caggcggtgg actggctggc gtcgctgcgt
     1441 ctgccggtga tccgttacgc gctgcaggat tattcctttt tcttcgcacc gcaaaatctg
     1501 gtgcggcggt tcctcaacca gttgcatctc aacctgctca gcagttccca caaaagtcgt
     1561 cagatcctgc gtgatgccct ggcgcatttc attcgccgta tccagcagga tttccggcag
     1621 gacgtgagcc agttcaacag tgtctgcatc gaagcccagt cctggtttgc cggccaggac
     1681 gacaaggtgg ggcagatcga gcaacagctg cggtcgctgg aatccgtgcg ccgcaaggag
     1741 cgggtggcgg aaccccgcgt ggtgcaggaa ctgaaccgca tcgcggccgg gaaggcactg
     1801 ccgcgccagc tggcggaatt cctgcacggc gagtggcgca agtcgttgcg gctggtgtcg
     1861 atgcgggagg gggaggaagg tactacctgg aaacggcagg tgcgcatcac cgaatccctg
     1921 gtggaattcg cactcgagtg cgtggatgcc aaggggcgtg acaagcaccg cagcttctat
     1981 ccggtcatga tgaagaatat ccgctccctg ctggtcagcg tgagtgacga tccagcagcg
     2041 ctggaagcgg cgctcgatcc gctggaactg gtgttgatgg ccctgctcag tggcgccaat
     2101 cttgaagcgg tgaaggtgcc ggcactgcag ctgaccagtg cccaggtcac gatggcggaa
     2161 gtcaagcagg tgagtccccg ggcattggcc gaaatccagg ccctgcagac cggggcctgg
     2221 ttgcgggtca agaccaccga tggcagccac gaactgtgcc gcctgacaat caaaagcagt
     2281 gatgatgagc cctgggtgct ggtcagccag agcggcaaga caattatcaa gaaaaccgca
     2341 tttcagctgg cacaggggct ggaaggagga gtgctcgaac ggattggcca gacccactgg
     2401 tgggatgaaa aactcgatac tgtgctggcg gacctgcatg tgcgctggca gcgctggcag
     2461 acggagcagc cggctgcccc cgcgccccag ccggaatccc gcattgatat ccttctgaat
     2521 acgccgtcga cgccgccagc accaaaggcg cctgtggttg cagcgcccgc gatcaaggtg
     2581 ccggaggtga ccgcggaaag tccgcctgcg gtcgcggagg ttgccgccgc ttcatcgcag
     2641 acgcccgacg cggcgtccgt cccggtggca gaaaccggcg gcgtcgatgc tgtcgatttt
     2701 ctggcgccgc gccccgtcag cgaggcggag cgcaacgctg ccctgcaggc catcgaccag
     2761 ttgcaggtgg gcggctggat tctgcagcaa acctccaaag gcgaggaacg ttgtaaactg
     2821 gcagtgcgca tccgcggccg cgagaagctg gtgtttgtga accgtctggg cgtcaaggtg
     2881 ctggagatcg accgcgtacc cctggccgaa ttgctggcgc gcggcctggt gaccatcatt
     2941 gatactggag tcaagttcaa caatacgctg gaacgggttg ttcgcagcat acaggaagaa
//
LOCUS       DASUEG010000690         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1951993, whole genome shotgun sequence.
ACCESSION   DASUEG010000690 DASUEG010000000
VERSION     DASUEG010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951993"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..936)
                     /locus_tag="VFX11_13025"
     CDS             complement(34..936)
                     /locus_tag="VFX11_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4939594.1"
                     /translation="MASESNYYIPENSWMPVLTAASLGSMALGAGLLVQGKSPLFLLA
                     GSLVLACVLFNWFSTVIQENMRGMTSAQVKRSFVIAMGWFIFSEVMFFAAFFGALFYV
                     RVLAVPWLGGEGDKAMTNALLWQGFTANWPVMQTPDQALGGTTFVGPHENMGFPGWSK
                     LAGWLPLWNTILLLTSSWTVHIAHHAMKHGNRGRFNFFLAVTVALGLAFAVVQYVEYH
                     EAYTEMGLTLKSGIYGTTFFMLTGFHGFHVCLGAFMLAIQLLRGLKGHFKPDDQFGFE
                     ASSWYWHFVDVVWLGLFIFVYVLG"
     gene            complement(979..1533)
                     /locus_tag="VFX11_13030"
     CDS             complement(979..1533)
                     /locus_tag="VFX11_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX4939595.1"
                     /translation="MAANNPVRKTVLWSLLAGVAMFGFAMFVMPPLYDALCEITGLGG
                     KTSGRYVADQPAVDGERVVKVQFVTTNNSAMPWEFRPLTDAVRVHPGESHKIEFYARN
                     DTERDMVAQAIPSVTPANAAQYLQKTQCFCFDQQPLKAGAEANMPVVFFIDPALPSSV
                     NTITLSYTLFDVTDRQSVAVTARN"
     gene            complement(1543..>2187)
                     /locus_tag="VFX11_13035"
     CDS             complement(1543..>2187)
                     /locus_tag="VFX11_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX4939596.1"
                     /translation="GELYFMYATMLIAVPTGVKVFNWITTMFRGAITYETPMLFAIAF
                     VILFTLGGFTGLMLAIAPADIQYHDSYFVVAHFHYVMVAGALFSSTAAVYYWIPKWTG
                     RMYNETMGKVHFWVSFVAFNVTFFPQHFLGLAGMPRRYADYSLQFADWNMVSSIGAFA
                     YGASQLLFLYNVIATIVAGRKISDPKVWEGAEGLEWTVPTPAPYHTFSTPPAIR"
BASE COUNT          511 a          648 c          621 g          407 t
ORIGIN      
        1 cctggccgtt gccgttgcgt gcgcggatgt ctttcagccg agcacgtaca cgaagatgaa
       61 cagaccgagc cagaccacat cgacaaaatg ccagtaccag gacgacgcct caaagccgaa
      121 ttgatcgtcc ggcttgaaat gccccttgag acctcgcaac agctgaatcg ccagcatgaa
      181 ggcacccagg cagacgtgaa agccgtggaa tcccgtcagc atgaagaagg tcgtgccgta
      241 aatgccggac ttcagtgtca gccccatttc tgtataggct tcgtgatact ccacgtactg
      301 aacaacggcg aatgcgagtc caagcgcgac ggtgacggcc aagaagaagt tgaaacggcc
      361 gcgattgccg tgcttcattg cgtggtgggc gatatgcacc gtccagctcg acgtcagcag
      421 cagaatcgta ttccacagtg gcagccatcc ggcaagcttg ctccagcccg gaaagcccat
      481 gttttcatga ggacccacaa aggtggtgcc gcctagcgcc tgatcggggg tctgcatgac
      541 cggccagttc gccgtaaagc cctgccacag cagcgcattg gtcattgcct tgtcgccctc
      601 gccacccagc cagggcaccg ccagtactcg cacatagaac agcgcaccga aaaacgcggc
      661 aaaaaacatc acttcggaga agatgaacca gcccatggct atcacgaaag atcgtttcac
      721 ctgcgcgctg gtcatgccgc gcatgttttc ctgaataacc gtgctgaacc agttgaacag
      781 cacacaggcc agaaccaacg aacccgccag caggaacaac ggggactttc cctgcaccaa
      841 gagcccggcg ccgagtgcca tgctgcccag ggacgctgcc gtcagcacag gcatccagct
      901 gttttcggga atgtagtaat tactctcgga tgccatgtgt ttctccttaa gaatgcggcg
      961 cccttggcga ccggccaatc agttgcgcgc cgtcacggcg acggattgcc ggtcggtgac
     1021 atcgaacagg gtgtaggaca gggttatggt gttgacgctg gacggcaacg cgggatcgat
     1081 aaagaatacc accggcatgt tggcttctgc gccagccttt aatggttgct ggtcgaaaca
     1141 gaaacattgg gttttttgca gatactgcgc tgcattggct ggcgtcacac tgggaatagc
     1201 ctgcgccacc atgtctcgtt cggtatcgtt gcgggcgtag aactcgatct tgtgcgactc
     1261 acccggatgc acccgtaccg catcggtcag cggtctgaac tcccaaggca tggcgctgtt
     1321 gttggtagtg acaaactgta ctttcaccac tcgctcccca tcgaccgcgg gctgatcggc
     1381 cacataacgc ccactggttt tgccgccaag gccggtaatc tcacacagcg cgtcgtataa
     1441 cggcggcatc acgaacatcg caaatccgaa catggcgacg ccggccagca gcgaccagag
     1501 gactgtcttt ctgaccggat tgttcgccgc cataagctag gcttaccgga tcgcgggcgg
     1561 ggttgagaac gtatggtagg gcgcgggcgt cggcaccgtc cactccaggc cttcggcgcc
     1621 ttcccacact ttcgggtcgc tgatcttgcg gccggccacg atggtcgcga tcacgttgta
     1681 aaggaacagt aattgcgacg ccccgtaagc aaaggcaccg atgctggaga ccatgttcca
     1741 gtcggcgaat tgcagcgaat aatccgcata gcgccgcggc atgccggcca ggccgaggaa
     1801 atgctgcggg aaaaaggtga cgttgaaggc gacgaaagac acccagaaat ggactttccc
     1861 catggtctcg ttgtacatcc ggcccgtcca cttcggaatc cagtaataga ccgccgcggt
     1921 gctggagaac agcgcacccg ccaccatcac atagtggaag tgagccacca caaagtagct
     1981 gtcgtggtac tggatgtccg ccggagcaat cgccagcatc aatccggtga agccaccgag
     2041 cgtaaacaga atcacaaaag cgatggcaaa caacatcggg gtttcgtagg tgatggcacc
     2101 gcgaaacatg gtggtgatcc agttgaacac cttcacgcca gtgggcaccg cgatcaacat
     2161 ggtggcgtac atgaagtaca gctcgcc
//
LOCUS       DASUEG010000691         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1082700, whole genome shotgun sequence.
ACCESSION   DASUEG010000691 DASUEG010000000
VERSION     DASUEG010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082700"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFX11_13040"
     CDS             complement(<1..697)
                     /locus_tag="VFX11_13040"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="quinoprotein glucose dehydrogenase; catalyzes the
                     formation of D-glucono-1,5-lactone from D-glucose and
                     ubiquinone; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HEX4939597.1"
                     /translation="MAGIVVRKKAFMKSTYRSSSSSFHGICRGSLLLVVLTLSQALTA
                     NESAATESAVDQHQLGTKYSPLNQITKDNVTNLEVAWEYHTGDGAHPKPGSLTSFQDQ
                     PSLIEGNLVVCSTHRRVIALDPKTGQERWVFDPKDPETGMKKCRGVANWVDSQAADGT
                     ACRTRIFLGTADYRLFAIDAKTGKPCEGFGDQGQVKMPVDKPEIFTGEVLANSRPAIV
                     NDVVVMGSTVADNQ"
     gene            complement(776..>2177)
                     /locus_tag="VFX11_13045"
     CDS             complement(776..>2177)
                     /locus_tag="VFX11_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4939598.1"
                     /translation="WVDPVVLKSLDVENPATEKVCGRVAIGSAADVDKAVKAARKAFA
                     SWSQTAASDRIEILQNILVEYQKRSGDLAAALTEEMGAPRYLANGFQVGLGVGHLSTA
                     IDVLKKFQFEELRGATLIVKEPIGVCGLITPWNWPLNQIAVKVFPALATGCTVILKPS
                     EIAPFTGQIFAEIMHAAGVPAGVFNLVQGDGPGVGVALSSHPDIDMVSFTGSTRAGVE
                     IARNAAPSVKRVTQELGGKSPSIILDDEAFAKNVAGGIGSIMGNSGQTCSAGSRLLVP
                     KVRMKEAINIARQAASQVTVGDPNGDVIMGPVSSKVQFDKIQGLIQKGIDEGATLVAG
                     GVGRPEGLKTGYFVKPTVFADVTNDMTIAREEIFGPVICILGYDNIEQAIEIANDTEY
                     GLAGYVAGVNLEKARAVARKIRAGWVSINDGFDFNCPFGGYKKSGNGREWGEFGFHDF
                     LEIKGILGYAPEQTTQ"
BASE COUNT          472 a          635 c          573 g          497 t
ORIGIN      
        1 gctggttgtc ggccaccgtc gatcccatca caacgacgtc gttcacaatg gcagggcggg
       61 agttggcgag cacctctccg gtaaaaatct cgggcttgtc gactggcatt tttacctggc
      121 cctgatcacc gaatccttca cagggtttgc ccgttttggc atcgatcgcg aagagccgat
      181 agtctgccgt gccgaggaaa atgcgcgtcc tgcatgctgt gccgtcggcc gcctgcgaat
      241 caacccagtt ggcaacacca cgacatttct tcatgccggt ttcgggatct ttcggatcga
      301 acacccaacg ctcctggcca gtcttcgggt ccagcgcaat cacgcgacga tgcgtactgc
      361 ataccaccag attgccttcg ataagactgg gctggtcctg aaatgacgtc agcgagcccg
      421 gcttcggatg cgcgccatcg ccggtgtgat attcccaggc cacttccaga ttggtcacgt
      481 tgtcttttgt gatctggttc agtggagaat acttggtgcc aagctgatgt tgatccactg
      541 ctgattcagt agcggcagac tcattggcgg taagtgcctg ggacagtgtc aacaccacca
      601 aaagcaggct gccccggcaa atgccgtgaa aagaggaaga actggaacga tacgttgatt
      661 tcataaatgc ttttttcctg acgacaatac ccgccacaat ttggtgtcag catagtgaca
      721 ccaaataaaa tggacgtcaa atggacggat ttattgctgg gctatgatga ttttgttatt
      781 gggttgtctg ctcgggcgca taaccgagga tgcccttgat ctccagaaaa tcgtggaatc
      841 caaattcgcc ccattcacga ccattgccac tcttcttgta accgccgaag ggacaattga
      901 agtcgaaacc gtcattgatg gacacccatc ctgcgcgaat ttttcgggca acggctcttg
      961 ccttttcgag gttcacgccc gctacatagc cggccaggcc atactcggta tcgttggcga
     1021 tctcgatggc ctgctcgata ttgtcgtagc ccagaataca gatgactggc ccaaaaattt
     1081 cctcgcgggc gatggtcatg tcattggtga catcggcaaa aacggtgggt ttgacaaaat
     1141 aacctgtctt cagaccctca gggcgaccca ctcctccggc aaccagcgtg gcgccttcgt
     1201 cgatgccctt ctggatcaat ccctggatct tgtcgaactg aacttttgag gacacgggcc
     1261 ccatgatgac gtcgccattg ggatcaccca ctgtcacctg cgacgctgct tgccgcgcga
     1321 tattgatcgc ttctttcatg cggactttgg gcaccagcag gcgtgaaccg gcgctgcagg
     1381 tctggcctga gttgcccatt atgctgccaa taccgcccgc gacatttttc gcgaacgctt
     1441 cgtcatcgag gatgatgctg gggctcttgc cacccagttc ctgggtaacc cgtttaacgg
     1501 acggcgctgc gttgcgtgcg atttcaacgc cggcacgggt cgaaccggtg aaggaaacca
     1561 tgtcgatatc cggatgagag gaaagtgcta cacccacccc agggccatca ccctgaacca
     1621 gattgaatac tccggccgga acgccagcgg catgcatgat ttcagcaaaa atctggccgg
     1681 taaaaggtgc aatttccgat ggcttgagaa tcacagtgca accggttgcc agtgccggga
     1741 aaaccttgac ggcgatctgg ttcagcggcc agttccaggg cgtgatcaga ccgcagacgc
     1801 cgatcggttc cttcacgatc agcgttgcgc cgcgcagctc ttcgaactgg aattttttca
     1861 gtacgtcgat agcagtcgac aggtgaccca cgccaagccc aacctgaaaa ccgttggcca
     1921 gatagcgcgg cgcacccatc tcttcggtca gcgccgcagc cagatcacct gagcgcttct
     1981 gatattccac aaggatgttc tgcagtattt caatccggtc cgacgcggca gtctgcgacc
     2041 agctcgcaaa agccttgcgg gctgccttaa ccgccttgtc cacatccgct gctgagccaa
     2101 tcgccacccg gccacacact ttttcggtgg caggattttc cacatccagg gatttcagta
     2161 cgaccggatc gacccat
//
LOCUS       DASUEG010000692         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1532711, whole genome shotgun sequence.
ACCESSION   DASUEG010000692 DASUEG010000000
VERSION     DASUEG010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532711"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFX11_13050"
     CDS             complement(<1..605)
                     /locus_tag="VFX11_13050"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX4939599.1"
                     /translation="MKKQAYGWILLALWGLLLTGTSPARATEVGGLYKASVAVKDRSD
                     AERRRAFAEGFVQVLVRASGDSGIGQQEAVVTAIGSVDKYMMQYGYATRDGESGEPQP
                     VLNMDFDGSAINEFLRRYGLPLWGSNRPPLLIWMAWEQELDRDLVSAEANPGAHQILQ
                     QEAVRRGVVLSFPAFDSEDRSRVSIGDVWGLFPEPVLAASAR"
     gene            817..1884
                     /gene="purM"
                     /locus_tag="VFX11_13055"
     CDS             817..1884
                     /gene="purM"
                     /locus_tag="VFX11_13055"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4939600.1"
                     /translation="MSKQPTTPSANPPSLSYKDAGVDINAGGELVERIKGVAKRTKRP
                     EVLGGLGGFGALCEIPSKYKQPVLVSGTDGVGTKLRLAIDLKKHDTVGIDLVAMCVND
                     LIVTGAEPLFFLDYYATGHLDVDTAASVITGIGKGCELSGCALVGGETAEMPGMYEGD
                     DYDLAGFCVGVVEKSRIIDGSKVQAGDTLIALASSGPHSNGYSLIRKIIEVAKADLNQ
                     TLDGKPVSQLLMEPTRIYVKNLLTLIEQVEVKALAHITGGGLLENVPRVLPEFTNAVI
                     DTKSWELPAVFKWLQQNGNVASREMYRTFNCGVGMVVCVAADQADKTLQALAKLGEKA
                     WVVGQIVASEQQEPETILGGI"
     gene            1892..2545
                     /gene="purN"
                     /locus_tag="VFX11_13060"
     CDS             1892..2545
                     /gene="purN"
                     /locus_tag="VFX11_13060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX4939601.1"
                     /translation="MNPVNIVVLISGSGTNLQAIIDAVERGEINGRIAAVISNRPDAK
                     GLERAQQHGLHTVVIDHRTFSGRDTFDAALQATIEQFQPALVVLAGFMRILTDGFVQH
                     FSGRLLNIHPSLLPKFKGLNTHARALEAGEQEHGCSVHFVSCELDGGAVIGQARVAIA
                     ADDTADTLAAKVQRLEHRLYPACVKLFCAGQLQLTDNGALLNGKPLPLNGLDLTEAP"
     gene            2545..>2777
                     /locus_tag="VFX11_13065"
     CDS             2545..>2777
                     /locus_tag="VFX11_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939602.1"
                     /translation="MLTLSRILIALLLAGVALTASAETTAKNALQPFTAHYKGRANGF
                     AVGDLGARELKALGGGRYRLQYTAEALIYTLQET"
BASE COUNT          569 a          916 c          816 g          476 t
ORIGIN      
        1 cgggcggacg ccgccagcac cggttccgga aacaggcccc acacatcgcc gatggaaacg
       61 cgtgagcgat cctcgctgtc gaaggcaggg aaagacagca ccacaccgcg ccgcacggct
      121 tcctgctgca ggatctggtg agcgcccggg ttggcctcgg cactgaccag gtcgcggtcc
      181 agttcctgtt cccaggccat ccagatcaac agcggcggcc ggttgctgcc ccacaggggc
      241 aggccgtagc ggcgcagaaa ttcattgatg gcgctgccat cgaagtccat gttgagtacc
      301 ggctgcggct cgcctgattc accgtcgcgg gtggcatagc catactgcat catgtacttg
      361 tcgacgctgc caatggcggt caccacggct tcctgctggc caatgccgga atcaccgctg
      421 gcccgcacca gcacctggac aaagccttca gcaaaggcac gccgccgttc agcgtcgctg
      481 cgatccttca cggccaccga tgccttgtac agaccgccga cctccgtcgc ccgggccggg
      541 ctggtgcccg tcagcaacaa cccccacaac gccagcagaa tccacccata cgcctgtttt
      601 ttcataacgt cctggcaagc ctcgagaatc atgtaaacgc cgcagtttac aacgtcgccc
      661 agggcgataa aaccgaaacc tgtgtcgccg tgccggcctg ccccggcgcg acgattgggc
      721 ttgcccgcaa ctcctgatag aatacgcccc ctgtttgccg gctcaggcca gcgtgcgacc
      781 ccacagttcc tgcaacccat acggacccag cgccccatgt caaagcaacc gaccacgcct
      841 tccgccaacc cgccttccct cagctataaa gatgccggtg tcgatatcaa tgccggtggc
      901 gaactggtgg aacgcatcaa gggcgtggcc aagcgcacca aacgccctga agtactgggc
      961 ggactggggg gcttcggtgc cctgtgcgag attccttcca aatacaaaca accggtgctg
     1021 gtgagcggca ccgacggcgt gggcaccaag ctgcgcctgg ccatcgacct gaaaaaacat
     1081 gacaccgtgg gcattgacct ggtggccatg tgcgtcaacg atctgatcgt caccggtgcc
     1141 gaacccctgt ttttcctcga ctactacgcc accggccatc tggacgtgga caccgccgcc
     1201 agcgtgatca ccggcattgg caagggctgc gaactgtccg gctgcgccct ggtgggcggc
     1261 gagaccgccg aaatgcccgg catgtacgaa ggcgacgatt atgatctggc gggcttctgc
     1321 gtgggcgtgg tggaaaaatc ccgcatcatc gacggcagca aggtgcaggc cggcgacacg
     1381 ctgatcgcac tggcctcttc cggtccccat tccaacggct actcgctgat ccgcaaaatc
     1441 attgaagtgg ccaaagccga cctgaaccag accctggacg gcaaacccgt cagccagctg
     1501 ctgatggaac ccacccgcat ttacgtgaag aacctgctga cactgatcga acaggtggaa
     1561 gtaaaagcgc tggcccacat caccggcggc ggtctgctgg agaacgtgcc gcgcgtgctg
     1621 ccggaattca ccaacgccgt gatcgatacg aaatcctggg aactgccggc cgtgttcaaa
     1681 tggctgcagc agaacggcaa cgtggccagc cgcgaaatgt accgcacctt caactgcggc
     1741 gtaggcatgg tggtctgcgt ggccgccgat caggccgaca aaaccctgca ggcgctggct
     1801 aaactgggtg aaaaagcctg ggtggtgggc cagatcgtgg ccagcgaaca gcaggaaccg
     1861 gaaaccattc tgggcggcat ctgatccgtc catgaaccct gtcaacatcg ttgtactcat
     1921 ctccggcagc ggcaccaatc tgcaggccat catcgacgcg gttgaacgcg gcgagatcaa
     1981 tggccgcatt gccgctgtca tcagtaaccg tccggacgcc aaaggtctgg agcgggcgca
     2041 gcagcatggc ctgcacacgg ttgtgatcga tcaccggaca ttttccggcc gcgacacatt
     2101 cgatgccgca ctgcaggcaa caatcgagca gttccagccg gcgctggtgg tgcttgcggg
     2161 cttcatgcgc attctcactg acggcttcgt gcagcatttt tccggccggc tgttgaacat
     2221 tcacccttcc ctgctgccca agttcaaggg cctgaatacc cacgcccgcg cgctggaagc
     2281 cggcgagcag gaacacggct gcagtgtgca tttcgtcagc tgcgagctgg atggcggtgc
     2341 cgtgatcgga caggcccgcg tggccatcgc cgcagacgac accgccgaca cgctggcagc
     2401 caaggtacag cggctggagc accggcttta tcccgcctgt gtgaaactgt tctgcgccgg
     2461 gcaactgcaa ctcaccgaca atggcgccct gctgaacggc aagccgctac cgttaaatgg
     2521 acttgatctg accgaggcac cctgatgctg acgctttccc gaattctgat cgccctgctg
     2581 ctggcaggcg tggcgctgac tgccagtgca gaaactaccg ccaagaatgc actgcaaccc
     2641 ttcaccgccc attacaaggg ccgtgccaac ggattcgccg tgggcgatct gggcgcgcgt
     2701 gaactgaagg cactgggggg cggccgctac cgcctgcaat acacggccga ggcgctgatc
     2761 tatacgctgc aggaaac
//
LOCUS       DASUEG010000693         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1333490, whole genome shotgun sequence.
ACCESSION   DASUEG010000693 DASUEG010000000
VERSION     DASUEG010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333490"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFX11_13070"
     CDS             complement(<1..946)
                     /locus_tag="VFX11_13070"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX4939603.1"
                     /translation="MISPNDWKQQALAQTGQERSLHWLLQPAIAQAVFRAAELPTRKT
                     ERWKYTPVTALLQQDFLQPAAADAVDEGLARDCFADLPDASRIVLVNGQLNRALSDFS
                     DLENQGVITLFSMASPEQRELVNMHLGSALNPEKHLFAALNYCHLQDGLLLHAPALQK
                     LAQPVYVVYLGSALNTPAWAQTRLLVVAEPGAELQLIEQFDSVGEQNNLFHNHVTEIV
                     LQPNARLQHIRLQVEQEALSHINGLHVKLARDSHYEQHGIAFGSQLKRNDINVNFAGE
                     NAACVLNGVFLSKHRQHIDNHLNLEHATPLCSSNTAFKG"
     gene            complement(959..1696)
                     /gene="sufC"
                     /locus_tag="VFX11_13075"
     CDS             complement(959..1696)
                     /gene="sufC"
                     /locus_tag="VFX11_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX4939604.1"
                     /translation="MLIVKDLVAEVDGKTILNGISLTVKPGEVHAIMGPNGSGKSTLA
                     NVLAGRDGYEVKSGEVIFQGHNLLEQAPEERAREGVFLAFQYPVEIPGVSNMQFLKAS
                     VDAIRKHRKLPELDTPAFMKMARDKCKDLKLNPDFLKRGVNEGFSGGEKKRNEILQMA
                     LLEPSLCLLDETDSGLDIDALQVAAEGVNALRDGKRGFVLVTHYQRLLNYIVPDYVHV
                     LAGGRIVKSGGRELALELEEKGYSWIQ"
     gene            complement(1737..>2157)
                     /locus_tag="VFX11_13080"
     CDS             complement(1737..>2157)
                     /locus_tag="VFX11_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX4939605.1"
                     /translation="TGTKMIHLGKNTRSTIVSKGISAGRSQNAYRGLVRISPRADNAR
                     NHTQCDSLLIGDRCGAHTFPYIEAKNSDSIIEHEATTSKVSDDQLFLCRQRGIDEEKA
                     VSMIVNGFCKEVFKELPMEFAVEAGKLLEVSLEGAVG"
BASE COUNT          402 a          644 c          620 g          491 t
ORIGIN      
        1 aacccttgaa cgcggtattg ctggagcaga gcggcgtggc atgttccaga ttcagatgat
       61 tgtcgatgtg ctggcggtgc ttgctgagaa acacaccgtt cagcacgcaa gcggcatttt
      121 caccggcgaa attcacattg atgtcgttac gcttgagctg gctgccaaaa gcgatgccgt
      181 gctgttcgta atgactgtcg cgggccagtt ttacatgcag gccgttgatg tggctcaggg
      241 cttcctgctc cacctgcagg cggatatgtt gcagccgcgc gttgggttgc agcacgattt
      301 cagtgacatg gttatggaac aggttattct gttcgccgac actgtcgaac tgctcgatca
      361 gttgcagttc cgcgccgggc tcggcgacca ccagcaaacg ggtttgcgcc caggccggtg
      421 tgttcaacgc gctgcccaga taaaccacgt acaccggctg cgccagtttt tgcagtgccg
      481 gggcgtgcag cagcaggcca tcctgcagat ggcagtaatt cagggcggca aacaggtgtt
      541 tttccggatt caaggccgaa cccagatgca tgttcaccag ttcgcgctgt tccgggctgg
      601 ccatgctgaa cagggtgatc acgccctggt tctccaggtc gctgaaatcc gacagcgcgc
      661 ggttgagctg gccgttcacc agcacgatgc ggctggcatc gggcaggtcg gcaaaacaat
      721 cccgggccag gccttcgtca acagcatcgg ccgccgccgg ctgcagaaag tcctgttgca
      781 gcagagcagt aaccggcgtg tacttccagc gttcggtctt gcgggtgggc agttcggcgg
      841 cgcgaaacac ggcctgcgcg atcgcgggtt gcagcagcca gtgcagggat ctttcctgtc
      901 cggtctgcgc cagcgcctgt tgtttccaat cgttcggtga aatcatgact gcctgccgtt
      961 actgaatcca gctgtaacct ttctcttcca gttccagcgc cagttcgcgc ccgccggact
     1021 tcacgatcct gccacccgcc agtacatgca cgtagtcggg tacgatgtaa ttcagcagac
     1081 gctggtaatg ggtgaccagc acaaaaccgc gcttgccgtc gcgcagggcg ttgacacctt
     1141 ccgccgcgac ctgcagcgca tcgatatcaa gcccggaatc ggtttcatcc agcaggcaca
     1201 ggctgggttc cagcaacgcc atctgcagaa tctcgttgcg cttcttctca ccgccggaaa
     1261 aaccttcatt cacgccgcgc ttgagaaaat ccggattcag tttcagatcc ttgcacttgt
     1321 cccgcgccat tttcatgaag gcgggtgtat ccagttccgg cagtttgcgg tgcttgcgaa
     1381 tcgcgtccac cgaggctttc aggaattgca tgttgctgac gccgggaatt tccaccgggt
     1441 attgaaacgc caggaacaca ccttcgcggg cgcgttcctc cggagcctgc tccagcaggt
     1501 tgtggccctg gaaaatcact tcgccgcttt tcacttcata accgtcgcgg ccggccagca
     1561 cattcgccag cgtgcttttg ccggaaccgt tggggcccat gatggcgtgg acttcgccgg
     1621 gcttcacggt gagggaaatg ccgttgagga tggttttgcc atccacttct gctaccagat
     1681 ctttcacaat caacatcggg tctgtactca taatcaaatc aaccaacggc gctttctcag
     1741 cccaccgctc cttccagact cacttccagc agcttgccgg cttccaccgc gaattccatc
     1801 ggcagttcct tgaacacttc cttgcagaag ccgttcacga tcatcgacac ggctttttcc
     1861 tcgtcgatac cgcgctggcg gcagaggaac agctggtcgt cgctcacctt ggacgtcgtg
     1921 gcttcgtgct cgatgatgct gtcggaattc ttcgcttcga tatacggaaa cgtgtgcgca
     1981 ccgcagcgat cgccaatcag cagcgaatcg cactgggtgt gattgcgggc gttgtcggca
     2041 cggggtgaaa tccgcaccag gccgcgatag gcattctggc tgcgaccggc ggagatgcct
     2101 ttcgatacaa tggtgctccg ggtgttcttg cccaggtgga tcatcttggt gccggtg
//
LOCUS       DASUEG010000694         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1909988, whole genome shotgun sequence.
ACCESSION   DASUEG010000694 DASUEG010000000
VERSION     DASUEG010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909988"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFX11_13085"
     CDS             <1..518
                     /locus_tag="VFX11_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939606.1"
                     /translation="SWFILLIGAEIVQALTHFKTRKERSKSTLGNLLSILCQLYRHQG
                     EGNVVDEVDLLQRMSWISHEDWETYCDLLVRVQLASRNEEGHIMLTRDLHKYSLAQLF
                     RDCFGEVLELDLQSGDGWQQRALTLHAEGLKQCLTHWDLDLASLFDARVAPSPTAARE
                     SQTSPPAGSGG"
     gene            complement(466..2001)
                     /locus_tag="VFX11_13090"
     CDS             complement(466..2001)
                     /locus_tag="VFX11_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939607.1"
                     /translation="MSNPLQVPSIEKTRHILLETDSRRLTTEIRDLPYADIPSALLMA
                     HRRLHTFNRFSLPPAKRLELVRPFHYAFVRFTEHYRRQFESSVFSREPNLHELDNLLE
                     FLRELGFAFKHLIRDSLERNKRPAGLGLLLYMALNYLHYHALFSCNRGRLLKPAFWLE
                     VHYLYFTAADLQLELENLQTPEGKQASIEQLYKQIILIGLSSPFSLSPEEQWRTFDYV
                     ARFGHLTDIGLVTDASALQESYSLTRDCSQPALLPGENAATRDTRLLNLAPFIDSLQR
                     HMAAVKGGEALRIVGMERVQRRLVLDLLAKLHNNWTRNPERQGDRQPIDESIGLVWGL
                     ESICAMLDPALRRRDSLLNRPATPDNRAWAKGENESPNGIRLHLDDSEEPHPDAGQVV
                     ALIRMHDDQKVLQIGLVQWCARDQNDQPFFGVQRLHGTARKVTICQQDDQETERNGLL
                     VVAKTADGRVRSLLMAPTGTLQPGGRVQVYSTHSATPNHVEAFAVTHRTRQVETFEIR
                     VLQ"
     gene            2177..3151
                     /locus_tag="VFX11_13095"
     CDS             2177..3151
                     /locus_tag="VFX11_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939608.1"
                     /translation="MKITLESSLSLEGIKPYFLQNVCGKRTFSAQLFGESGPIVVCLH
                     GFPDNYLTFRYQVPALVRAGYRVLLPVLPGYEPASVDPEGRHQITELVRWLEGWLDFL
                     GEEEEKVHLVGHDWGAAVTWLAAVMIPGRLHSITSIAIPSLHHLARGVLRHPGQLRKS
                     WYMGFFQLPLLPERALVMGDGWLIRRLWRNWSPGWDAPDGQLYSVCHDLQQPGVTAAA
                     LGYYRCLFRLFDPENQAGRALLARKVDLPTLMISGGRDGCLDTRLFDEGLVDADFAGG
                     VELYRLMGAGHFCHLEKPALVNRKLLSFLQAHTPGSVRQAGELASQLG"
     gene            3234..>4523
                     /locus_tag="VFX11_13100"
     CDS             3234..>4523
                     /locus_tag="VFX11_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX4939609.1"
                     /translation="MAVIRQDDFIQSIADSLQYISYYHPLDFIQAVNEAYEKEESIAA
                     KDAMAQILINSRMCAEGHRPICQDTGIVTVFLKVGMNVQWDAKMSVTDMVNEGVRRAY
                     NNPDNVLRASILSDPAGARKNTKDNTPAVIHYEIVEGDTVDVQIAAKGGGSENKSKMV
                     MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKESLMEPVDIHELK
                     ARGPQNRIEELRLELFEKVNALGIGAQGLGGLTTVVDVKILDYPTHAASLPVAMIPNC
                     AATRHVHFTLDGSGVAELEVPKLEDWPQIAMTGGNKAKRVNLDTVTAEDVQSWQPGDT
                     LLLSGTLLTGRDAAHKRMVDMLNKGETLPVDFKGKFIYYVGPVDPVRDEVVGPAGPTT
                     ATRMDKFTRQMLEQTGLLGMVGKAERGPAGIEAIRDNK"
BASE COUNT          879 a         1302 c         1434 g          908 t
ORIGIN      
        1 tgagctggtt catcctgctg atcggtgccg agatcgtgca ggcgctcacc catttcaaaa
       61 cccgcaaaga gcgttcgaaa tccacattgg gcaacctgct gtcgatcctg tgccaactgt
      121 accggcacca gggcgagggc aatgtggtgg acgaagtcga cctgctgcag cgcatgtcct
      181 ggatcagcca cgaggactgg gaaacctact gtgacctgct ggtgcgggtg caactggcca
      241 gccgcaatga agagggtcac atcatgctga cccgggatct gcacaagtac agcctggcgc
      301 aattgttcag ggactgtttc ggcgaggtgc tggaactgga cctgcagtca ggcgatggct
      361 ggcagcaacg ggcgctgacg ctgcatgccg aagggttgaa gcagtgtctg acgcactggg
      421 atctggatct ggccagcctg ttcgacgcgc gggtggcccc atcgcctact gcagcacgcg
      481 aatctcaaac gtctccacct gccgggtccg gtgggtgacg gcaaaggctt ccacatgatt
      541 gggcgttgcc gaatgtgtgc tgtacacctg tacgcggccg ccgggctgca atgtaccggt
      601 cggtgccatc agtaatgagc ggacgcggcc gtcggcggtt ttcgccacca ccagcagccc
      661 gttgcgctcg gtttcctgat catcctgctg gcagatggtg accttgcgcg cagtgccgtg
      721 caggcgctgc acgccgaaga aaggctggtc gttctgatcg cgtgcgcacc attgcaccag
      781 accgatctgc aataccttct ggtcgtcgtg catgcggatc agcgccacca cctggccggc
      841 atccggatgc ggctcttcgc tgtcgtccag gtgcaggcgg atgccattcg gactctcgtt
      901 ttcccctttt gcccaggccc ggttatcggg ggtggccggc cggttcagca ggctatcgcg
      961 acggcgcagt gccgggtcca gcatggcgca gatgctttcc agcccccaca ccagcccgat
     1021 gctttcgtcg atcggctgcc ggtcaccctg acgttccgga ttacgggtcc agttgttgtg
     1081 cagtttcgcc agcagatcca gcaccaggcg ccgctgcacc cgctccatgc caacgatgcg
     1141 cagtgcctcg ccgcctttaa cggctgccat atggcgctgc aggctgtcga taaagggggc
     1201 caggttgagc aggcgggtat ccctggttgc agcgttctca cctggcaaca gcgccggctg
     1261 gctgcaatcg cgcgtcagcg aatagctttc ctgcaatgcg ctggcatccg tcaccaggcc
     1321 aatgtcggtg agatgaccga agcgggccac ataatcaaat gtgcgccact gttcttccgg
     1381 tgacaggctg aatggcgaac tcaggccgat caggatgatc tgcttgtaga gttgttcgat
     1441 gctggcctgc ttgccttccg gcgtctgcag gttttccagt tccagctgca ggtctgctgc
     1501 ggtgaaatac aggtagtgca cttccagcca gaaagcgggt ttcagcaggc ggccccggtt
     1561 gcaggaaaac agggcatggt agtgcagata attcagcgcc atgtacagca gcagaccaag
     1621 gccggctgga cgcttgttgc gttccagcga atcgcggatc aggtgcttga aggcaaagcc
     1681 cagctcgcgc aggaattcca gcagattgtc cagttcgtgc agattcggtt cgcgggaaaa
     1741 cacgctgctt tcgaactggc ggcgatagtg ttcggtaaag cgcacaaagg catagtggaa
     1801 aggccgcacc agttccaggc gttttgccgg cggcagggaa aagcggttga aggtatgcag
     1861 gcggcgatgc gccatcaaca gggccgaggg aatgtcggca tagggcagat cgcggatttc
     1921 cgtggtcagc cggcggctgt ctgtttccag caggatgtgc cgggttttct cgatggaggg
     1981 tacctgcaat ggattactca tgacatccga tccttcaatc gtcacgtgct tcgcccggca
     2041 gatgctgcgc ctatggctcc agcaatgctg ctcctggctg tacggctcgt atctgtactt
     2101 tgcaggatgg tttgggcatg cttgctgata accgtctcaa ctccgaggcg caggttccgg
     2161 acgcacaaat tacactatga aaataacgct agagtcgtcg ctttccctgg aaggaattaa
     2221 accctatttt ctccagaatg tctgtggcaa aagaacattt tctgctcaac tttttgggga
     2281 gagcggcccc attgttgtct gtttacacgg gtttcccgac aattatttga cattccgcta
     2341 tcaggtaccg gcattggtgc gggcgggcta tcgcgtgttg ctgcccgtgc tgccgggcta
     2401 tgagccggcc agtgtggatc cggaggggcg ccatcagatc accgaactgg tgcgctggct
     2461 ggaaggctgg ctggattttc tgggcgaaga agaagaaaag gtgcatctgg tgggccacga
     2521 ctggggcgcc gccgtgacct ggctggcagc cgtgatgatt cccgggcggc tgcacagcat
     2581 caccagtatc gccattccat ccctgcacca tctggcccgc ggcgtgttgc gtcatccggg
     2641 gcagctgcgc aagtcctggt acatgggctt tttccagctt cccctgctgc cggagcgggc
     2701 gctggtcatg ggggatggct ggctgatccg ccggctgtgg cgcaactggt caccggggtg
     2761 ggatgcacct gacggccagc tgtacagcgt gtgccacgac ctgcagcaac cgggcgtgac
     2821 cgccgccgcc ctggggtatt accgctgcct gttccgtctg ttcgatccgg aaaaccaggc
     2881 tggccgtgcc ctgctggcgc gcaaggtgga tttgccgacg ctgatgatca gcggtggccg
     2941 ggacggttgc ctggatacgc ggctgttcga tgaggggctg gtggacgcgg attttgctgg
     3001 cggcgtggag ttgtaccggt tgatgggcgc cggccatttc tgccatctgg aaaagcctgc
     3061 gctggtcaat cggaagttgc tgtccttttt gcaggcgcat acgccgggct cagtccggca
     3121 ggcgggtgaa ttggccagtc agttgggata gaatgcgggc gctttcaagc ccaagacaga
     3181 tcagagccaa agtccgtaga gagcccaagt cagtaaatag agaggtgcct gccatggccg
     3241 tcattcgcca ggacgatttc attcagagta ttgccgacag cctgcaatac atttcctatt
     3301 accacccgct ggatttcatc caggccgtca acgaggccta tgaaaaggaa gaatccatcg
     3361 ccgccaagga tgcgatggcc cagatcctca tcaattcccg catgtgcgcg gaaggccacc
     3421 gtcctatctg tcaggacacc ggtatcgtca ccgtttttct gaaagtgggc atgaacgtgc
     3481 agtgggacgc gaagatgagc gtcaccgaca tggtcaacga aggcgtgcgt cgcgcctata
     3541 acaatcccga caacgtgctg cgtgcctcca tcctgtctga ccccgctggc gcccgcaaaa
     3601 acactaaaga caacacaccc gccgttatcc attatgaaat cgtcgaaggc gacaccgttg
     3661 acgtgcagat cgcggccaaa ggcggcggtt ccgaaaacaa atccaagatg gtcatgctca
     3721 accccagcga cagcatcgtg gactgggtgc tgaaaaccgt tcccaccatg ggtgccggct
     3781 ggtgtccgcc gggcatgctg ggcatcggca tcggcggcac ggcggaaaaa gcgatgctgc
     3841 tggcgaaaga gtcgctgatg gagccggtgg atattcacga actgaaagcg cgcggcccgc
     3901 aaaaccgcat cgaggagctg cggctggaac tgttcgagaa agtgaacgcg ctgggcatcg
     3961 gcgcgcaggg tctgggtggt ttgaccaccg tcgtcgacgt gaagattctg gactacccga
     4021 cccacgccgc ctcgctgccg gttgcgatga ttcccaactg cgccgctacc cgtcacgtgc
     4081 atttcaccct ggacggcagc ggcgtggcgg aactggaagt gccgaaactg gaagactggc
     4141 cgcagatcgc catgaccggt ggcaacaagg ccaagcgcgt gaacctggat acggttaccg
     4201 cggaagacgt gcaaagctgg cagccgggcg acacgctgct gctgtccggc acgctgctga
     4261 ccggccgcga tgctgcccac aagcgcatgg tggacatgct gaacaaaggc gaaaccctgc
     4321 cggtggattt caagggcaag ttcatctatt acgtgggccc ggttgatccg gtgcgtgacg
     4381 aagtcgtggg tccggcaggc ccgacgaccg caacgcgcat ggacaagttt acccggcaga
     4441 tgctggagca gacgggcttg ctgggcatgg ttggcaaggc cgaacgcggc ccagccggca
     4501 tcgaggccat tcgcgacaac aag
//
LOCUS       DASUEG010000695         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_582658, whole genome shotgun sequence.
ACCESSION   DASUEG010000695 DASUEG010000000
VERSION     DASUEG010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582658"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..736)
                     /locus_tag="VFX11_13105"
     CDS             complement(125..736)
                     /locus_tag="VFX11_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX4939610.1"
                     /translation="MKLLQAMILGVLALVQAHSAFAFGVERFVEGVHYLRFDKVEKTP
                     NTVVEFFSLGCPHCAHLEPELEKWLATKPEAVKFARIPATWNPRFEFLARVYLALQAS
                     GQDQKLVPPLFDYLHKDQKPLTNVDEAVAFVKAQGGDAEGFRKAFDSSDRTTELKKSE
                     SLFMTYEMRGVPALLVNGQYTVSVSQAGGVAEMFEIVAFLLTK"
     gene            925..1908
                     /locus_tag="VFX11_13110"
     CDS             925..1908
                     /locus_tag="VFX11_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939611.1"
                     /translation="MFNLIKQGQTVARRLYNNRSHLREDMTLALQHAWTLPYLATPWS
                     AESLTRSELHPRSYNNWGDVINLSLQAMVPMERRTYSPGTVACYPEHYAVGAPREKWF
                     FINGICTSPPVAILNGLELAKAFNRPIHLIHTPTSGVLWDLWDSMTARTLRKDGLLGR
                     LAYDVVKEALRHHDKVVIIGHSQGTIVSSYIARKLLKDKRYRHHAPKLEIYCIAGVAD
                     SFRVDPHLSAHFGRGVPYVEHFANGLDFFCRIGVLAHLDNTSGAVFVLPNRTGHLLND
                     HYIPGIWRGEYCNKRSRLYKYIDGNTPASNDYVQHDLNMDADTPDARAEAS"
     gene            complement(1909..>2540)
                     /locus_tag="VFX11_13115"
     CDS             complement(1909..>2540)
                     /locus_tag="VFX11_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4939612.1"
                     /translation="FQRIGLRADIKLEHLLASSAIPAVFPAVRINREYFGDGAMRQLA
                     PISPALHLGARKVLVVGVSGNRSAPQVRKPMEGYPSLAQISGHLLNSVFLDSLEYDIE
                     RLQRINKTLDMIPESVRAKSAANLRPVGSLVISPTQSVDQIASKYLHVLPVTIRMFLH
                     GIGATRSSGSSILSYLLFHRGFCSELIALGYHDAMAKAEEIAEFMDQDD"
BASE COUNT          532 a          872 c          661 g          475 t
ORIGIN      
        1 ctccacgaca ccggatctgg ctccgccggc tccggccgga gcccccaccg catcgccgcc
       61 catcgatccc gatgcggtgg aagcctttcc ggacaccgaa agctgagccc ggcttaattg
      121 aggcttattt ggtcaacaaa aaagccacga tctcgaacat ctcggcaacg ccacccgcct
      181 gactgacgct gacggtgtac tgcccgttca ccagcaaagc cggtacaccg cgcatttcgt
      241 aggtcatgaa caggctctcg gatttcttga gctctgtcgt gcgatcactg gaatcaaacg
      301 ccttgcggaa accctccgca tcgccgccct gcgccttcac gaacgccacc gcctcatcga
      361 cattggtcag cggcttctgg tccttgtgca ggtagtcgaa cagtggcggc accagtttct
      421 ggtcctgccc gctggcctgc agcgccagat aaacccgggc cagaaattcg aagcgcggat
      481 tccaggtggc gggaatgcgc gcgaacttta cagcctcggg cttggtggcc agccactttt
      541 ccagctccgg ctcgagatgc gcacaatggg ggcagcccag gctgaaaaat tccaccacgg
      601 tattcggcgt tttctccacc ttgtcgaagc ggaggtaatg cacgccttcc acaaaacgct
      661 ccactccaaa tgcaaaagcg ctgtgggcct gcaccagcgc caacactccc agtatcatcg
      721 cctgcaacag tttcatgttc actccacgct atcagtttca aatcgtcgcc aagaatagca
      781 gcagccttgg ccaaatccta tcgactccgt tccagccaat cgcacccctg tcaatccccg
      841 aaccacgcgg cttattgacg ccagtggcct caccagggtc ccgctgcgcc tatactgaaa
      901 gctgaatgca gagcaggatg ccccatgttc aatctgatca aacagggcca gaccgtcgcc
      961 cgccgactct acaacaaccg cagtcacctc cgtgaggata tgactctggc gctgcagcac
     1021 gcctggaccc tgccttacct ggccacgccc tggagcgctg aatcccttac ccgctccgaa
     1081 ctgcatccgc gcagttacaa caactggggc gacgtgatca atctcagcct gcaggccatg
     1141 gtacccatgg agcgacgcac ctacagcccc ggcaccgtgg cctgttaccc tgagcattac
     1201 gctgtcggcg cgccgcgtga aaaatggttc ttcatcaacg gtatctgcac ctcgccgccc
     1261 gtcgccattc tcaacgggct ggaactcgca aaagccttca accgccccat ccatcttatt
     1321 cacacaccca cttccggcgt cctgtgggac ctgtgggatt caatgaccgc ccgcaccctg
     1381 cgcaaggacg gattgctggg ccggctggcc tatgatgtag tgaaagaagc gctgcgccat
     1441 cacgacaagg tggtgatcat cggccattcc cagggcacca tcgtcagcag ctacattgcc
     1501 cgcaaactgc tgaaggacaa gcgctaccgt caccacgcac ccaagctgga aatctactgc
     1561 attgccggcg ttgccgacag cttccgcgtc gacccgcacc tgagcgccca tttcggccgt
     1621 ggcgtaccct atgtcgagca cttcgccaac ggtctggatt tcttctgccg catcggcgtg
     1681 ctggcgcacc tggacaacac ctccggcgcc gtgttcgtgc tgcccaaccg caccggacac
     1741 ctgctcaacg accactacat tcccggcatc tggcgcggcg agtactgcaa caagcgctcg
     1801 cggttgtaca aatacatcga cggcaataca ccggcctcga acgattatgt gcagcatgat
     1861 ctgaacatgg atgccgacac gcccgacgcc cgtgccgagg ccagctgatc agtcgtcctg
     1921 atccatgaac tcggcaattt cctccgcctt ggccatggcg tcgtgataac ccagcgcgat
     1981 cagttcgctg caaaaaccgc gatgaaacag cagataactc agaatgctgg aaccggacga
     2041 acgggtggcg ccgatgccgt gcaggaacat gcggatcgtc accggcaaca catgcaggta
     2101 cttggacgca atctggtcca cgctctgggt gggcgaaatc accaggctgc ccaccggtcg
     2161 cagattggcc gcgcttttcg cccgcaccga ttccggaatc atgtccagcg tcttgttgat
     2221 gcgctgcaaa cgctcgatgt cgtattccag gctgtccagg aacacactgt tgagcagatg
     2281 cccgctgatc tgcgccagtg acggataacc ttccatcggc ttgcgtacct gcggcgccga
     2341 ccggttgccc gacacaccca ccaccagcac cttgcgcgcg cccaggtgca acgccgggct
     2401 gatcggcgcc agctgccgca tcgcgccgtc gccaaaatat tcgcggttga tgcgcacggc
     2461 agggaatacc gccggaattg ccgacgacgc cagcagatgc tccagcttga tgtccgcccg
     2521 cagacctatg cgctgaaaac
//
LOCUS       DASUEG010000696         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1812437, whole genome shotgun sequence.
ACCESSION   DASUEG010000696 DASUEG010000000
VERSION     DASUEG010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812437"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFX11_13120"
     CDS             <1..862
                     /locus_tag="VFX11_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4939613.1"
                     /translation="GWAAIPIRISDLMRVVLQGINNPNLPGSTVLLFDGDRPSQFHRL
                     YDPANIFGTPSLTHPNYQQIKVPGRTWLVGVQLTRALPGPDGLHWTQAVILLVGALLS
                     ALAALFSRELLLSQQRTMDANHQLTLAAEEQAIASAVFEGMADGVVITNPDGLVQSLN
                     QSFTQLTGYTNLDLRGRSLRILRSNKHADSLYQTLWNDLRSVGNWRHEIWNRCKDGRL
                     RLHDVSITTVIDKDLQPQHFVAHYQDVTDRHREQQLILYRATHDALTGLSNRTQLEDR
                     LEQTLALAQR"
     gene            872..1285
                     /locus_tag="VFX11_13125"
     CDS             872..1285
                     /locus_tag="VFX11_13125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939614.1"
                     /translation="MGLLFMDLDGFKAVNDHYGHDVGDALLIAVSRRLETFVRKTDTL
                     CRLGGDEFVLLLPQAPDLAGLLQLAERIQAEVRQPYTGVHENGALSISVSIGIASAPE
                     HGDDSEALMQAADRAMYGCKQSESQTPVFAAVNSP"
     gene            complement(1343..1591)
                     /locus_tag="VFX11_13130"
     CDS             complement(1343..1591)
                     /locus_tag="VFX11_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939615.1"
                     /translation="MLLNPFCPFTAARVAALKATVVLLLTLVIKAKIRITAFSALGFV
                     LLLLQTLLFLALSAGLGLAAGGAALTVNQQRRSSAAAG"
     gene            complement(1667..2812)
                     /locus_tag="VFX11_13135"
     CDS             complement(1667..2812)
                     /locus_tag="VFX11_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4939616.1"
                     /translation="MGLSLLINSCQSTPNHPPTGAACLPSSPVAKQCRGVVIRNNPDR
                     PSSTPPQLPVQSFNPTFTAIVGRTPKLVPLATGFGFLEGPVFLKDSTGPGGLVLMTDQ
                     IHNSIYAMRWNGLVRGQIAPQSWEKPTLFRNPSGVADGQTADQQGNLLTAETTGRRVS
                     ITRAFSANRRSPDPQAPTLIGTFAGKRLNSPNDLVVKTDGSIWFTDPSYGSLQFPPQP
                     ADLPNNVYRYDPRTHQLSVVENSLMMPNGIAFGPNEATLYIIDSAAIQGPGTYFSDLP
                     HNIYVYDVANNGRTLTNKRLFATIGPGFPDGMRLDEYGNLYVGTLEGIHVLNPKGLLI
                     GKLLLGRQTANLTFGGANNDILFIGSSDTLWAIKLNTRGLVPVRTRS"
BASE COUNT          697 a         1024 c         1080 g          673 t
ORIGIN      
        1 gggctgggcg gctatcccga ttcgcatcag tgatctgatg cgtgtggttc ttcagggcat
       61 taacaacccc aacctgcctg gatccaccgt ccttttattt gatggtgatc gcccaagcca
      121 gtttcatcgg ttgtacgatc cggccaatat cttcggtaca ccaagtctca cccacccgaa
      181 ctaccagcag attaaggtgc ccggacgcac ctggctggtg ggggtgcaac tcacccgagc
      241 cttgccaggg ccggacgggt tgcactggac tcaggccgtg atcctgctgg tgggtgctct
      301 cctttcagcg ctcgccgcac tcttcagccg tgaattgctc ctgagccaac agcgcacgat
      361 ggacgcgaat catcagctga cccttgctgc tgaggagcaa gccatcgcct cagccgtctt
      421 cgagggaatg gcggacggtg ttgtgatcac caaccccgat ggattggtgc aatcactcaa
      481 tcagagcttc acccagctga cgggatatac caacctcgat ctgcgcggtc gctcgcttcg
      541 catcctccgt tcgaataaac atgccgacag cctctatcag accctctgga acgatctgcg
      601 ttctgttggc aattggcgcc acgaaatctg gaatcgatgc aaagacggcc gactccggct
      661 acatgatgtc agcatcacga cggtgattga caaagactta cagccacagc attttgtggc
      721 gcattatcag gacgtcaccg atcgtcatcg cgaacagcag ttgatcctct accgagccac
      781 tcatgatgca ctgacgggcc tgtcgaaccg cacccagttg gaggatcgac tggagcagac
      841 actggcactc gcgcagcgtt aacacaccaa ggtgggactg ctgtttatgg acctcgatgg
      901 ctttaaagca gtgaatgatc attacggcca cgatgttggc gatgcactgc tgatcgcagt
      961 cagccgcagg ctggaaacct ttgtgcggaa gactgatacc ctctgtcgcc tgggcggcga
     1021 tgaattcgtt ctcttactgc ctcaggcacc ggacctggct gggcttctgc agctggccga
     1081 gcggatccag gccgaggttc gacagccata cacgggtgtg catgagaacg gggcgctctc
     1141 catttcggtg agcatcggga tcgccagcgc gccggagcat ggcgacgaca gcgaggccct
     1201 catgcaggcc gccgatcggg ccatgtacgg ctgcaagcag agcgaatcgc agaccccggt
     1261 gttcgccgcg gtcaacagtc catgagtcct gtggccatcg ctggcacaca gcaggcccat
     1321 cgagcccgtt cgacctcctg gctcagccag cggcggctga tgatcggcgc tgctggttca
     1381 cggtcagcgc ggctccacca gctgccagtc ccaagcctgc actgagggcc agaaagagca
     1441 gggtttgcag gagcagcaga acaaagccca gggccgagaa ggccgtgatc cggatcttgg
     1501 ccttgatcac cagggtgagc aggagcacca ccgtggcctt gagggcggca accctggccg
     1561 cggtgaacgg gcagaacgga ttcagcaaca cagggtgacc ccaacctggc cattctggcg
     1621 gggtgaccag tggactgaga ggtccccccg gccggcgcca gcgccatcaa gaccgggtgc
     1681 gcacgggaac cagaccgcgg gtgttcaact tgatggccca gagggtgtca ctcgagccaa
     1741 tgaacagaat gtcattgttg gcaccgccaa aggtgagatt ggcggtctgc cgaccgagca
     1801 gcagcttgcc gatcaacagg cctttgggat tcagcacatg aatgccttcg agggttccca
     1861 cgtagagatt gccgtactca tcaaggcgca tgccatcggg aaagccgggg ccaatggtcg
     1921 caaacaggcg tttgttggtg agcgtgcgtc cgttgttggc gacgtcatag acatagatgt
     1981 tgtgcggtag atcagagaaa taggtgcctg gaccctgaat cgcagcactg tcaatgatgt
     2041 aaagggttgc ctcattgggg ccaaaggcga tgccattcgg catcatcagg ctgttctcaa
     2101 caacgctcag ctgatgggtg cgcgggtcat agcggtaaac attgttcggc agatcggcgg
     2161 gctgtggagg gaactgcaga gagccataac tcggatccgt gaaccagatg ctgccatcag
     2221 tcttgacgac aagatcattg ggtgaattca ggcgcttgcc cgcaaacgtg ccgatcagcg
     2281 ttggggcctg tggatcaggg ctgcgccgat tcgcgctgaa cgctcgggtg atcgagaccc
     2341 gccgtcctgt ggtttctgcg gtgagcaggt tgccctgctg atcggcggtc tgcccatcgg
     2401 caactcccga cggattgcgg aacagcgttg gcttctccca ggattgcggt gcgatctgcc
     2461 cacgcaccag accgttccag cgcatcgcat agatgctgtt gtggatctga tcggtcatca
     2521 gcaccagccc gccaggcccg gtgctgtcct tgaggaagac cgggccctca agaaagccga
     2581 atcccgttgc cagcggcaca agcttgggcg tacgcccgac gatcgccgtg aaggtggggt
     2641 tgaaggattg aaccggcaac tgtggcggcg tggagctggg gcgatcgggg ttgttgcgga
     2701 tcaccacgcc acgacattgc ttggcgacag ggctcgacgg caggcacgct gcacccgtgg
     2761 gtgggtggtt cggtgtgctc tggcagctgt tgatcagcag gctcagtccc atgccacacc
     2821 cggccaagaa cgcccgctgc aagcttgttc cgcttcgcga gatcagccac cacggatggt
     2881 gcgaaccgca gcggggttgg gcagcactgc cgctcatctg agggtgcttc atgcgcaggc
     2941 cactccgagt ggacggaaca ccccatggga cgcgcaagcg cgagcgccat cggcgcgatc
     3001 ggcgttgtcg cgcgggtcaa ccggagaggt gatgggcatg gacctcagaa ctgccatcgg
     3061 aatcacgccg gcatccactc aggcctcgtc ctctgtgctg ccacgagggg ctcgttgatg
     3121 aggaaaagcg ccgcgcaagc ggcaccggtc tcccctgacc gtccgccagg agcggcggcc
     3181 gatcacacgc atcgtgacgc gttggccagg taggcggcag gaagctgctg gatgaccaaa
     3241 gctttacacg ggaccatggc ggaagatcac aacagattgc agctgatttc tgcagcttgc
     3301 gttctgtgcg gcgaatcggg ggaacaacac ttcagggtct ctcaccatcc aggatgaagg
     3361 cagcactgct ctcatccctt tgctatccca gatcgttgct cctgcaccag tcggttgtgc
     3421 tgagccaatg caggtggttg gcgatgcgtt gcgatatttc ggcctgcatt tccg
//
LOCUS       DASUEG010000697         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_254690, whole genome shotgun sequence.
ACCESSION   DASUEG010000697 DASUEG010000000
VERSION     DASUEG010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254690"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFX11_13140"
     CDS             complement(<1..203)
                     /locus_tag="VFX11_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939617.1"
                     /translation="MPHKIRHAPLKTLFFPPPWCMPRVILGTTPPTPSPRQAAPHDNS
                     LFTDSSLAATSAMRTFWSIAILR"
     gene            510..1808
                     /locus_tag="VFX11_13145"
     CDS             510..1808
                     /locus_tag="VFX11_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4939618.1"
                     /translation="MANRDELKAAVNEAGLEKDVDVSRRQFLKGSMAAVGAATMMGGM
                     SMSMSSTAWAAGSDKPEKPVVNVGFIPLTDCASVVVAALLGFDKKYGIKINVSKEASW
                     AGVRDKLINGELDAAHVLYGLIYGVHMGIGGPKKDMSVLMTLNQNGQAITLSNQLKAK
                     GVSDGKSLKKLIDTDKREYTFAQTFPTGTHAMWLYYWLSANGIDPMRDVKTIVVPPPQ
                     MVANMRVGNMDGYCVGEPWNARAIFDNIGFTAVTTQEIWADHPEKVLGCTGDFAKKYP
                     NTARALIMAVMDAARYIDDVKNRPQVAKLISGKSYVNAPEDVIKERFLGNYDNGNGKK
                     WKDANPMQFFDGGKVGFPYHSDGMWFLTQHKRWGMLKTDPDYLGIAKQINQVELYKQA
                     AAQLKVPVPSSTMRSSTLIDGKVWDGSNPAAYAASFAIKM"
     gene            1931..2791
                     /gene="ntrB"
                     /locus_tag="VFX11_13150"
     CDS             1931..2791
                     /gene="ntrB"
                     /locus_tag="VFX11_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HEX4939619.1"
                     /translation="MTALMTEVEKAAVAITPSRVEKARAWLAAVNWGAVIARTVAPVL
                     GIILFLFVWSAVSHYRVDLPGPAATWEAAVALFSDPFYQNGPNDQGIGWNILRSLGRV
                     GMGFGMAALIGIPLGFMIGRFRFLNDMTSPIISLLRPVSPLAWLPIGLLVFKAAEPAS
                     IWVIFISSIWPIIINTAVGVSQVPQDYLNVARVLNLSEWKIFTKILFPSVLPYLLTGI
                     RLSIGVAWLVIVAAEMLTGGVGLGFWVWDEWNNLNVAHIIIAIFVVGIVGLLLEQALI
                     LIAKRFTYES"
     gene            2806..3600
                     /locus_tag="VFX11_13155"
     CDS             2806..3600
                     /locus_tag="VFX11_13155"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4939620.1"
                     /translation="MKKIVSIENAGMSFKTKKGMFTALRDIDLAIGEGEFVSLIGHSG
                     CGKSTLLNLIAGLTLPTNGVLLCNNREIAGPGPERAVVFQHHSLLPWLTCFENVYLAV
                     ERVFGEKEGRAQLKERTEKALNLVGLSHALKKMPGEISGGMKQRVGIARALAMEPRIL
                     LMDEPFGALDALTRAHLQDELIKIVAKTGSTVVMVTHDVDEAVLLSDRIVMMTNGPAA
                     TIGEILNVELARPRDRMQLANDSEYHHYRAEVLRFLYQGQKHPSAA"
BASE COUNT          745 a          997 c         1147 g          786 t
ORIGIN      
        1 atgcgcaaga tggcgatgga ccagaacgtg cgcattgcgg acgtggcggc caaactgctg
       61 tcggtgaaca agctgttgtc atgaggagcg gcctgtcgcg gtgatggggt tggtggtgtt
      121 gtccccagaa tgacgcgagg catgcaccac ggtggtggga agaacagggt ttttaacggt
      181 gcatgccgga ttttgtgggg catggcgtgc atcggaatgg tgaaaacaga gcggaaagcg
      241 ccgttttcac ccggaattca aaagttggca cgctcgatgc aatacacctg ttaacgagac
      301 cgatctccaa tgctggaacg gattcgtgaa tgtcgtgaga cattgccaag cgttgttgag
      361 cctgtgttgt tgtgttcccg gtgaacggga caatttgaat ccggtggtaa caccctgtgg
      421 acaatggcgt cctgcggaga aatctgcaga cgccattttt tttggcccgg agccgtcgaa
      481 tgaagacgct ctgcaaaccg aggtgaatga tggctaatcg tgatgagctg aaggcagcag
      541 tgaatgaagc cggtttggaa aaggacgttg atgtcagtcg ccgccagttt ctgaaagggt
      601 caatggcggc cgtgggggct gccacgatga tggggggaat gtccatgtca atgtcgtcca
      661 cggcctgggc cgcgggatca gacaagccgg aaaagccggt ggtgaatgtc ggtttcattc
      721 cactgaccga ctgcgcgtcg gtggtggtgg ccgctttgct cggcttcgac aagaagtacg
      781 gtatcaagat caatgtcagc aaggaggcct cctgggccgg cgtgcgtgac aagctcatca
      841 atggcgagct ggatgccgcc cacgtgctgt atggcctgat ttacggcgtg catatgggca
      901 tcggtggtcc gaagaaagac atgtcggtgt tgatgacgct taaccagaac ggccaggcca
      961 tcacgctgtc caaccagctc aaggccaagg gcgtgtctga cggcaagtcg ctgaagaaac
     1021 tcatcgacac cgacaagcgc gaatacacct ttgcccagac cttcccaacc ggtacgcacg
     1081 ccatgtggtt gtattactgg ctgtcggcca acggcattga tccgatgcgc gatgtgaaaa
     1141 ccatcgtggt gccgccccct cagatggtgg ccaacatgcg cgtgggcaac atggatggtt
     1201 actgcgtggg tgagccgtgg aatgcgcgtg ccatcttcga caatatcggt ttcaccgctg
     1261 tcactaccca ggaaatctgg gccgatcacc ccgagaaggt gctgggctgt accggcgatt
     1321 tcgcaaagaa atacccgaac actgcacgtg cgctgatcat ggcggtgatg gatgcggcgc
     1381 gttacatcga cgacgtgaag aaccgtccgc aggtggccaa gctgatttcc ggcaagtcct
     1441 acgtgaatgc accggaagac gtgatcaagg aacgtttcct gggcaactac gacaacggca
     1501 acggcaagaa gtggaaggat gccaatccga tgcagttctt tgacggcggc aaggtgggct
     1561 tcccctatca ctccgacggc atgtggttcc ttacccagca caagcgctgg ggcatgctga
     1621 aaaccgatcc ggattatctg ggcattgcca aacagatcaa ccaggtcgag ctctacaagc
     1681 aggcggcggc gcagctcaag gtgccggtcc cgtcttccac gatgcgttca agcacgctga
     1741 tcgacggcaa ggtctgggat ggttccaacc cggcggccta cgcggcgagc ttcgccatca
     1801 agatgtaagg ctacgtcacg tcatgtcgct gtgacgtgac ctttccgaaa cgatgaatcc
     1861 ccgttgcgcg ctcgcgcgca acgcggcctc ttgctgcctg aaaacgcccc tggcgtaaaa
     1921 ggagactgac atgacagcac tcatgaccga agtggaaaaa gcagcggtgg caattacgcc
     1981 atcgcgtgtc gagaaagccc gtgcttggct agccgctgtg aattgggggg cagtcatcgc
     2041 tcgtacggtg gcgcctgtgc tcgggataat tctcttcctg ttcgtgtggt cggccgttag
     2101 ccattaccgt gtcgatctgc cggggccggc ggcgacctgg gaagctgccg tggcactgtt
     2161 ctccgatccg ttctaccaga acggtccgaa tgaccagggt attggctgga acatcctgcg
     2221 ttcgctgggt cgcgttggca tgggctttgg catggcggcg ctgatcggca ttccgctggg
     2281 tttcatgatc ggtcgcttcc gtttcctgaa cgacatgacc tcgccgatca tctcgctgct
     2341 acgtcccgtt tcgccgctgg cctggctgcc aatcggcctg ctggtgttca aggctgccga
     2401 gccggcctcg atctgggtga ttttcatctc cagcatctgg cccatcatca tcaacaccgc
     2461 cgtgggcgtg tcgcaggtgc cgcaggatta cctgaacgtg gcccgcgtgc tgaatctctc
     2521 cgaatggaaa atcttcacca agattctctt cccgtccgtg ctgccctatc tgctgaccgg
     2581 tatccgtctg tccatcggtg tggcgtggct ggtgatcgtg gccgcagaaa tgctcaccgg
     2641 tggtgttggc ctgggcttct gggtatggga tgagtggaac aacctgaatg tggcccacat
     2701 catcattgcg atttttgtgg tgggcattgt cggtctgctg ctggaacagg cgctcatcct
     2761 gatcgccaag cgtttcacct acgagagctg agtgggagag ccatcatgaa gaagattgtt
     2821 tccatcgaaa atgcgggcat gagcttcaag accaagaagg gcatgttcac ggcgctgcgc
     2881 gacattgatc tggccattgg tgaaggcgag tttgtatcgc tgatcggcca ctccggctgt
     2941 ggcaagtcca cattgctcaa cctcattgca gggctcacat tgccgactaa cggtgtactg
     3001 ctctgcaaca accgtgaaat tgccgggccg ggtccggagc gcgccgtagt gttccagcac
     3061 cattccctgc tgccatggct gacctgcttt gaaaacgtgt acctggccgt tgaacgtgtg
     3121 ttcggtgaga aggaaggccg cgcccagctg aaggagcgta ctgagaaggc gctcaatctg
     3181 gttggcctgt cgcacgccct gaagaaaatg cctggcgaaa tttccggggg catgaagcag
     3241 cgtgtgggca tcgcccgtgc gctggcgatg gagccgcgta tcctgctgat ggatgagcct
     3301 tttggtgcac tggatgcgct gacgcgtgct cacctgcagg acgagcttat caagatcgtg
     3361 gccaagaccg gatccacggt ggtcatggtg acacatgatg tggatgaggc cgtgctgctg
     3421 tcggaccgca tcgtgatgat gaccaacggc cctgccgcca ccatcggcga aatcctcaat
     3481 gtcgaactgg cgcgtccgcg tgatcgcatg cagctcgcca acgacagcga gtatcaccac
     3541 taccgcgccg aagtgctgcg gttcctgtac caggggcaga agcacccgtc agcggcctga
     3601 tgccttaaca cagagttttt ctccggtgcg gtccccgtcg tgagaggtcg gggaccgcac
     3661 cacgggcccg gcttg
//
LOCUS       DASUEG010000698         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2151305, whole genome shotgun sequence.
ACCESSION   DASUEG010000698 DASUEG010000000
VERSION     DASUEG010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151305"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFX11_13160"
     CDS             complement(<1..229)
                     /locus_tag="VFX11_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939621.1"
                     /translation="MNIPNKIIPGSDNDMQFVRQHLIDPESCLCCGSCEAACTEGAIQ
                     TDRLMQRYVVDPAKCNNSHACLDACSSNAIKT"
     gene            complement(226..1653)
                     /locus_tag="VFX11_13165"
     CDS             complement(226..1653)
                     /locus_tag="VFX11_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4939622.1"
                     /translation="MDKRVRITVEDLSEPLLIPADAYISKDYAEAEADRLWAKVWQQA
                     GRVEEISEVGSFITYEICNDSILIVRSAPDTIKAFHNVCTHRGRRLVSTPRGAHSAAG
                     KQKKFVCGYHAWTFDTTGKATYILDKSDWKGQLTEMCTNLREVKVDTWGGWIFINMDP
                     DCEPLREFLEPVASLLDPFEFEQMRYRFRAWGIFDCNWKVALEAFLEPYHVAGTHPQL
                     TKYGDFYAWSRALGRHGHDGYDTKEHKTAGQDVAADTSVHRAAKGEDARISTAQMQQE
                     FWDTVGAATTETLVKAAQRLVDELPAGTPAAEVHHHWLESARRDDAERGVIWPDISDK
                     QMSDAGLAQSIFPNMNFLPGPTFLLYYRVRPYGTDPDKCIYEAIALDRFPPGQQPQTE
                     WQFLEQDPDRWPYVIGQDISNMIEVQKGLKSRGFAGNLPNPWQERKVSNLHRNLAEYM
                     GMGEPQLLESALLESELPASELRGQ"
     gene            complement(1669..>2989)
                     /locus_tag="VFX11_13170"
     CDS             complement(1669..>2989)
                     /locus_tag="VFX11_13170"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4939623.1"
                     /translation="RWRISTRQGDDIRARNVILANGLLNIPKLPGIPGIDKFKGHLFH
                     TARWDYDYTGGDQKNPVLDKLSSKRVAIVGTGATAIQLVPYLGKYAKQFYVLQRTPSC
                     VDTRRNSATDPAWVKTLKPGWQAERRANFHRGANESFQKGEKDLICDIWTEVNRNLAA
                     EREEQDWPELTHEQLFARYEDMDFRVMERLRRRVDAIVRDKATAEKLKPYYRYQCKRP
                     TSNDEYYDTFNRPNVSLIDVSESQGVERLTETGFVANGVEYPVDCIVFASGYEVTSDL
                     DRRWGFDVFEGRNGLSMYQNWKGSYQTFHGVMTHGFPNLFVIGLNQGGLNSSLTVNFE
                     EQARHIAYIILQTMARGATSVEPSQEAQNAYVQHIRDTAIDRTEWIRACTPSYFNNEG
                     KPDVDENGNEKYRFYLGEVYGPGWDAFMQRLEHWRSQGDMQGLSLSQ"
BASE COUNT          586 a          835 c          858 g          710 t
ORIGIN      
        1 aggttttgat ggcgtttgaa ctgcaggcat ccaggcaggc atgactattg ttgcatttcg
       61 ccggatcgac cacataccgt tgcatcaggc ggtcggtttg aatggcacct tcagtgcagg
      121 cagcctcaca actgccgcag cacaggcaac tttccggatc aatcagatgt tgcctgacaa
      181 actgcatgtc gttgtcgctg ccgggaatga tcttgttggg aatattcatt gtccacgcag
      241 ctccgatgca ggaagttctg attcaagcag cgccgattca agcagctgcg gttcacccat
      301 ccccatgtac tccgccagat tgcggtgcag attgctgacc ttgcgttctt gccacggatt
      361 gggaaggttg ccggcgaaac cgcgggattt caggcctttc tgcacctcga tcatgttcga
      421 gatatcctgg ccgatgacat aaggccagcg gtcggggtcc tgttccagga attgccattc
      481 ggtctggggt tgctgccccg gcgggaaacg atcgagcgca atcgcctcgt aaatgcattt
      541 gtcgggatcg gtgccgtaag ggcggacccg gtagtaaagc aaaaaggtag gccctggcag
      601 aaaattcatg ttggggaaaa tgctttgcgc cagcccggca tccgacatct gcttgtcgga
      661 aatgtccggc cagatgacac cgcgttccgc atcatcgcgg cgtgcggatt cgagccagtg
      721 atggtgcact tccgcagccg gcgtgcctgc tggtaactca tccaccagtc gttgtgcagc
      781 tttcaccagg gtttcggtgg tggcagcgcc cacggtgtcc cagaattcct gctgcatttg
      841 cgcagtggaa atgcgtgcat cctcaccttt ggccgcgcgg tgcacactgg tatcggccgc
      901 cacatcctgt cctgcggttt tgtgttcctt ggtgtcataa ccatcatggc cgtgacgacc
      961 cagggcccgg ctccaggcgt agaaatcccc atacttggtg agctgaggat gtgtgcccgc
     1021 cacgtgatag ggctccagga acgcctccag cgcgactttc cagttgcagt caaaaatgcc
     1081 ccaggcgcga aagcggtaac gcatctgctc gaattcgaac ggatcaagca gggacgcaac
     1141 cggttccaga aattcgcgca ggggttcgca gtcaggatcc atgttgatga agatccagcc
     1201 gccccaggtg tcgaccttca cttccctgag gttggtgcac atctcggtga gctggccttt
     1261 ccagtcggac ttgtcgagga tataggtggc cttgccggtc gtgtcaaagg tccaggcgtg
     1321 atagccacag acaaattttt tctgtttgcc agcggcactg tgagcgcccc tgggtgtgct
     1381 caccagtctg cgtccgcggt gggtgcacac attgtggaag gctttgatcg tgtcgggtgc
     1441 actgcgcacg atcaggatcg aatcgttgca gatttcgtaa gtgatgaaac tgcctacctc
     1501 ggaaatttcc tccacccggc ctgcctgctg ccacactttg gcccacagcc gatcggcttc
     1561 cgcttcggcg tagtctttgg agatataggc atccgccgga atcagcagcg gttccgacaa
     1621 atcttcaaca gtgatcctga ctcgtttatc cacgacacac ccctgatatt attgtgacag
     1681 gctcaagcct tgcatatcgc cctggctccg ccagtgttcc aggcgttgca tgaaagcgtc
     1741 ccagccaggt ccataaacct cgcccagata gaaacgatat ttctcgttgc cgttttcatc
     1801 cacgtccggt tttccctcgt tattgaaata actcggggtg caggcgcgaa tccactcggt
     1861 gcgatcgatg gcggtgtcgc ggatgtgttg cacgtatgcg ttctgcgctt cctgagatgg
     1921 ctccacgctg gtggcacccc gcgccattgt ttgcaggatg atgtaggcga tatgacgggc
     1981 ctgttcttcg aagttcaccg tgagtgatga attcaagccg ccctgattca ggcctatcac
     2041 gaacaggttg ggaaaaccgt gggtcatgac gccgtggaag gtttggtagg agcccttcca
     2101 gttctgatac atcgacaacc cgttgcgccc ttcgaacacg tcaaatcccc agcgccggtc
     2161 cagatcgctg gtaacttcat agccgctggc aaacacgatg cagtccaccg gatattcaac
     2221 gccattggcc acgaaaccgg tttccgtcag gcgctcgacg ccctgggatt ccgacacgtc
     2281 gatcaggctg acattgggac gattgaacgt gtcgtaatat tcgtcattgg aggtcgggcg
     2341 tttgcactgg tagcggtaat agggtttgag tttttcggcg gtggctttgt ccctgacgat
     2401 ggcatcgacc cgccgccgca ggcgttccat cacacgaaaa tccatgtctt cataacgcgc
     2461 gaataattgt tcatgcgtca gttccggcca atcctgctct tcgcgttcgg ccgccaggtt
     2521 gcgattgacc tcggtccaga tatcacagat cagatctttt tcgcctttct gaaaactttc
     2581 gttggcaccg cgatggaagt tggcgcgacg ctcggcctgc cagcctggct tcagggtttt
     2641 gacccaggcc ggatcggtgg ccgagttgcg gcgggtatcg acgcacgagg gcgtgcgctg
     2701 cagaacatag aactgcttgg cgtatttgcc caggtaaggc accagttgaa tcgcggtggc
     2761 accggttccg acaattgcca cccgcttgct ggacagcttg tcgagcacgg gatttttctg
     2821 gtcgccaccg gtgtagtcgt aatcccagcg tgcagtgtga aaaagatggc ctttgaattt
     2881 gtcgataccg ggaatgccgg gtaatttcgg aatattcaaa aggccgttgg ccaggattac
     2941 attgcgggcg cggatatcgt cgccctgccg ggtgctgata cgccagcgt
//
LOCUS       DASUEG010000699         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1411959, whole genome shotgun sequence.
ACCESSION   DASUEG010000699 DASUEG010000000
VERSION     DASUEG010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411959"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..668)
                     /locus_tag="VFX11_13175"
     CDS             complement(204..668)
                     /locus_tag="VFX11_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4939624.1"
                     /translation="MLIARHRVVAFHYVLKNEQGEELDSSRGQLPLLYLHGHGNILPG
                     LEQALEGHSVGDHFTVTLRPEQGYGLRDPELVQRVPRTALEGTSELQLGMQFQAQTED
                     GEIPVRVIGLDAEHITVDGNHPLAGQTLQFEVEIRNVREALAEELEEGVADA"
     gene            complement(727..1311)
                     /locus_tag="VFX11_13180"
     CDS             complement(727..1311)
                     /locus_tag="VFX11_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEX4939625.1"
                     /translation="MHPTDSTEQLSPKGMSRKAFLKLTAGVALAAGFSPMLAGCSNNG
                     RALRFKTINDILQELALIEVNQPVTMQQPWSLYKVLQHMAQSMEYSMTGYPQMDSAAM
                     QAVAKLAFNTFKAQGYMSHDLGAVVPGAPEIPADGPVEESMLRFRNAASDFQNFTGVL
                     FPHFSYGNLSYADWELAHAFHAADHFSSITYTTV"
     gene            complement(1454..2344)
                     /locus_tag="VFX11_13185"
     CDS             complement(1454..2344)
                     /locus_tag="VFX11_13185"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF003706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEX4939626.1"
                     /translation="MEHWDEGVYMKRANRSPVGPPLILSCGVHGDERAPVQVLQQLLT
                     ELQSRTLMPVRPCLVIFANLLALEEKRRFLRHNLNRLFGAPQRVLGYEGERVVALEQH
                     CRDFAEMYGPGWHLDLHATIKPSRHPHFALRPHCADPQGARQYDPRWPATLAQAGFTA
                     LVQQNTPAHTFAHFSSSQLGYASFTLECGDIAGSDTQAGAPLERLLRPLLTAPELPEG
                     SERLQVYPVAVDLIKQHPDFRFLVDEKSDNFTPFAAGTPIAQDGATTYFAPDGGGTLL
                     FANSQVPVGDRAGLLLGPPG"
     gene            2560..4389
                     /locus_tag="VFX11_13190"
     CDS             2560..4389
                     /locus_tag="VFX11_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4939627.1"
                     /translation="MKLRYLVLAGALAQAGLVHGMGLGELELQSYLGNPLQADLRIVA
                     PGQFDQDDIRVRIARPEVYERFGARFEAFHNQIDFTVQPSPGGIRVQANSKGRVQEPF
                     LDIVVELSWPQGTAYRRYSLLVDPPAYAARWQKTAPADRPLETAAIALAQQPPARRPA
                     AARAAESTRQPVLAPSRAEGAHPYVVQSGDSLWKIARRLQRGSGTSIPALMDQLLAAN
                     PRAFIDGDPARLRQGVTLYLPADAAAIADVADAQPVKTAHAAVETAPSTTRAPVTPAP
                     VNVAPRDQTIDIQAELTSAQQAGQAAVPETLEEIRAALAQLEREKAELRQFQAQVKQD
                     MVQVLEQRIAVSQALLQAEMRQRRLEPAAETPSAGVVSTPSTPAAEPVAAEVAPVTLS
                     TLLAPRKPAPPRLETATLNAQDLLQSEPAASNLLVSRSGPNLWYLLAMLPLGLLVVLL
                     GMRSQRVHKIRQSEVLKDGELYELVFGSRRDRSRTDSPDQVRKALEQIREKAGHAEQP
                     VAPPATAMGNPDQGVSRDELKQMIDLYMLYSQYQKAQNVILTEISKRPARTDLRLYLM
                     QVYAAMNDWKAFDDQLEVLRRMGNAQLLEEALKLRSSAQPMQN"
     gene            complement(4477..>5699)
                     /locus_tag="VFX11_13195"
     CDS             complement(4477..>5699)
                     /locus_tag="VFX11_13195"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX4939628.1"
                     /translation="FLNENTAWWDGSQIYGSSQAQQNKLRTFQDGKMILVNGRLPMDA
                     QSGLPITGFNKNWWVGLELMHTLFVKEHNAIADMLKQNYPNWTDERLFQTARLINSAE
                     MAKIHTVDWTPAILKNFTLREAMFANWRGILPMWVPRLGDPATDGIMGGEKDLTNVPF
                     SLTEEFTAVYRMHPLLRDSLEVRNYANDAVIGNSLLQAHSFAGAAQLTSQHGLTNLFY
                     SFGRTHPGLLELNNYPQMLQNFTREPGVAMTDLGAVDILRDRERGIPRYNEFRRQIGL
                     NPIKDFTDITPNTAMAEKLRQIYNNDIEAIDVMIGSYAEGYRPPGYGFGETSFQIFIA
                     MASRRLLADRFFTDDYRPEIYTQQGLDWIDNVTFTRVLVRHMPELSTRLAKVENGFFP
                     WDEENEWFELKVRK"
BASE COUNT         1073 a         1685 c         1827 g         1114 t
ORIGIN      
        1 ggcgcatatc agtgccacct atccggatcg cttgcagctg gaggatttgc aggactggaa
       61 acggtgcgaa gtgctgctgg ccagtgtgcg cggtatttac ggaatactgg gcctggagga
      121 ggcgctggtt tcgctgtaat acgcaatggg atgaaaaaac ggtcgaaaga tacagatagc
      181 cgcgtcggca agaccggccc ggttcaggcg tccgccacac cttcttccag ttcttccgcc
      241 agcgcctcgc gcacattgcg gatttcaact tcgaattgca gtgtctgccc cgccagcgga
      301 tggttgccgt ccacggtgat atgctccgcg tccagcccga ttacccgcac cggtatttca
      361 ccatcctcgg tctgggcctg aaactgcatg cccagctgca actcgcttgt gccttccagt
      421 gcagtgcgtg gcacgcgctg caccagttcc ggatcgcgca ggccataccc ctgttccggg
      481 cgcagcgtca ccgtgaagtg atcacccacc gaatgtcctt ccagcgcctg ttccagcccg
      541 ggcagtatgt tgccgtggcc gtgcaggtac aacaggggca attgtccgcg cgagctgtcc
      601 agttcctcgc cctgttcatt tttcagaaca tagtgaaacg caacaacgcg atgcctggca
      661 atcagcatgg gttcacccgg gaagtgagcg gcgatgccgg gttgggccgg tcatcgccgc
      721 aggagatcag acggtggtgt aggtgatgct ggagaagtgg tctgcagcat ggaaggcgtg
      781 ggccagttcc cagtccgcgt agctcaggtt gccgtacgag aagtgcggga acagtacgcc
      841 ggtgaagttc tggaaatcgc tggcagcatt gcggaaacgc agcatgcttt cttccaccgg
      901 gccgtcagca ggaatttccg gcgcgccggg cacaactgca cccagatcgt ggctcatgta
      961 accctgggcc ttgaaggtgt tgaacgccag tttggcaacg gcctgcatgg cggcggaatc
     1021 catttgcgga tagccggtca tggagtattc catgctctgc gccatgtgtt gcagcacttt
     1081 gtacagggac cagggctgct gcatggtgac cggctgattc acttcgatca gggccagttc
     1141 ctgcagaatg tcgttgatgg tcttgaagcg cagggcgcgg ccattgttgc tgcagccggc
     1201 gagcatcggg ctgaagccgg ccgccagggc cacaccagcg gtcagtttca gaaacgcctt
     1261 gcgggacatg cccttggggg aaagctgttc agtggaatcg gtcgggtgca taggagacat
     1321 cgctttatgg tttgttatgt ttattagggg ctttgatcgg gatgctgcgt cagatcgcaa
     1381 aagcatcagt caccattatc gtaaaaggcg cgggcggacc atgccccatc catagggagg
     1441 ggcggaccca cactcagccg ggagggccca gcagcaggcc ggcgcggtct cccaccggta
     1501 cctggctgtt ggcgaacagc agggtacccc cgccgtccgg ggcgaaatag gtggtagcgc
     1561 catcctgcgc gatgggggtg ccggcggcga agggggtgaa gttatcgctt ttctcgtcca
     1621 ccagaaagcg gaaatccggg tgttgcttga tgagatccac ggcgaccggg tagacctgca
     1681 gccgttcgga cccctccggc agctcgggcg ccgtcagcaa cgggcgcagc aatcgctcga
     1741 gaggggcgcc agcctgggtg tcgctgccgg cgatatcgcc gcattccagc gtgaagctgg
     1801 cgtagcccag ctgactgctg ctgaagtggg caaaggtgtg ggccggggta ttttgctgca
     1861 ccagggcggt gaagccagcc tgggccagtg tcgccggcca gcgcggatca tattgccgcg
     1921 ccccctgcgg gtccgcgcaa tgggggcgca gcgcgaagtg gggatggcgc gaaggcttga
     1981 tggtagcgtg caggtccagg tgccagccgg gtccgtacat ctcggcaaag tcccggcaat
     2041 gctgttccag tgccaccacc cgctcacctt cgtacccaag cacccgctga ggggccccga
     2101 acaaccggtt caggttatgc cgcagaaagc ggcgcttttc ctccagtgcc agcaggttgg
     2161 cgaagatcac caggcagggg cgcaccggca tcagggtgcg ggattgcagt tccgtcaaca
     2221 gctgttgcag tacctgaacc ggcgcccgtt catcgccatg tacgccgcag gacaggatca
     2281 ggggcgggcc aaccggcgag cggttggccc gtttcatgta gacaccttcg tcccaatgtt
     2341 ccattgcatt cattcctcag gcagtggcag cctagattgt aagacagtcg tcggccctgt
     2401 tgttgctttt ggttatctcc aggcggctgg acagcatgcg gggttatggg ctgaatggcg
     2461 actgctaaaa aaggcctggg tcaatagaat cgattaaaaa gtgagctaca gttaacacat
     2521 tcctatcgta gtccgctgca ggtggaattg aggcgttcga tgaagttgcg ttatctggtg
     2581 ttggcgggtg ctctggctca ggcaggcctg gtacatggta tggggcttgg cgagctggaa
     2641 ctgcaatcct atctggggaa cccgctccag gcggatctgc ggatcgtggc tccgggtcag
     2701 ttcgatcagg acgacatccg ggtccggatt gcccgccccg aggtgtatga gcgctttggc
     2761 gcccgcttcg aagcctttca caatcaaatc gatttcacgg tacagccgtc gcccggtggc
     2821 attcgggtgc aggccaacag caagggcagg gtgcaggaac ccttcctcga catcgtggtg
     2881 gaactgagct ggccccaggg gacagcctat cgccgataca gcctgctggt ggacccgccc
     2941 gcgtacgccg cccgctggca aaagacggcg ccggcagacc gtccgctgga aaccgcggcc
     3001 attgccctgg cacaacaacc gcctgctcgc cgtccagcgg cagcccgcgc cgcagagtca
     3061 acacggcagc ccgtgctggc gccgtcccgc gccgagggcg cccatcccta tgtggtgcaa
     3121 tccggtgact ctctctggaa gatcgcccgg cgcctgcagc gcggttcggg cacctccatt
     3181 ccggccctga tggaccagct gctggcggcc aatccccgcg ctttcatcga cggtgatccg
     3241 gcccggttgc ggcaaggtgt gacgctgtat ctcccggcag atgcagccgc tattgcggat
     3301 gttgcggatg ctcagcctgt gaaaaccgcg catgcggccg tcgagacagc tccgtccacc
     3361 acccgggcgc ccgtgacccc cgcccccgtt aacgtcgcgc ccagggacca gacgatcgac
     3421 atccaggccg aactgacgtc ggcacagcag gccggtcagg ccgcagtacc ggaaacgctg
     3481 gaggaaatcc gcgccgcgct ggcacagctg gagcgcgaga aagccgagtt gcgccagttc
     3541 caggcgcagg tcaaacagga catggtgcag gtgctggagc agcgcattgc ggtcagccag
     3601 gcactgctgc aggcagaaat gcgtcagcgc agactggagc cggcggccga gactccgtcg
     3661 gccggtgtcg tttccacacc gtccactccg gccgcagagc cggtcgcggc cgaagtcgcc
     3721 cccgtaacac tgtctaccct gctggcaccg cgcaagcccg cgccaccccg cctggagacg
     3781 gccaccttga acgcgcagga cctgctgcaa tcggaaccgg ccgcgtccaa tctgctggta
     3841 tcccgctcgg ggcccaatct ctggtatctg ctggcgatgc tgccgctggg cctgctggtg
     3901 gtgttgctgg gaatgcgctc gcagcgggtg cacaagatcc ggcagtcgga agtgctgaaa
     3961 gacggagagc tgtatgaact ggtgttcggt tcccgccgcg accgcagccg caccgattcc
     4021 cccgaccagg tgcgcaaggc gttggagcag attcgcgaaa aggctggtca tgccgagcag
     4081 ccggtggcgc cgcctgcgac cgcaatggga aacccggatc agggcgtcag ccgcgatgaa
     4141 ctcaagcaga tgatcgatct gtacatgctg tacagccagt atcaaaaggc ccagaacgtg
     4201 attctgactg aaatttccaa gcgtcctgcc cgtaccgatc tgcgcctgta tctgatgcag
     4261 gtctatgctg ccatgaacga ctggaaagcc tttgacgatc agctggaagt gctgcggcgc
     4321 atgggcaatg cgcagttgct ggaagaggcg ctgaaactgc gcagcagcgc gcagccgatg
     4381 cagaactgaa cctgcaggcc gccagcgact tccctggagc aaaaaaaagc cccgtgtgca
     4441 acacggggct ttttttctgg gtggagcctg acgggcttac ttgcgaactt tcagctcaaa
     4501 ccactcgttt tcttcatccc aggggaagaa gccgttttcc accttggcca gacgggtcga
     4561 cagttccggc atgtggcgaa ccagcacccg ggtgaacgtg acgttgtcga tccagtccag
     4621 accttgctgg gtgtagattt cagggcggta gtcgtcggta aagaagcggt ctgccagcag
     4681 acggcgtgac gccattgcga tgaagatctg gaacgaggtt tcaccgaagc cgtaaccagg
     4741 cggacggtag ccttccgcat aggaaccgat catcacgtcg atggcttcga tatcgttgtt
     4801 gtagatctgg cgcagcttct ctgccattgc agtattgggc gtgatatcgg tgaaatcctt
     4861 gatcgggttc aggccgatct ggcgacggaa ttcgttgtag cgcgggatac cacgctcacg
     4921 gtcacgcagg atgtccaccg cgcccaggtc tgtcatcgcc acacccggtt cgcgggtgaa
     4981 gttctgcagc atctgcgggt agttgttcag ttccagcaga cccggatgtg tgcggccgaa
     5041 ggagtagaac aggttggtca ggccatgctg gctggtcagc tgcgcagcgc cggcaaagga
     5101 atgggcctgc agcaggctgt tgccgatcac ggcgtcattg gcgtagttgc gtacttccag
     5161 gctgtcgcgc agcagcgggt gcatgcggta aacagcggtg aattcctcgg tcaggctgaa
     5221 cggaacgttg gtcagatcct tttcgccacc cataatgccg tcagtggcgg gatcgcccag
     5281 ccgcggtacc cacatcggca ggatgccgcg ccagttggcg aacatggctt cacgcagggt
     5341 aaagtttttc aggatcgccg gggtccagtc cacagtgtgg atcttggcca tttcggcaga
     5401 gttgatcagg cgagcggtct ggaacaggcg ctcgtcggtc cagttcggat agttctgctt
     5461 cagcatgtcg gcgatggcgt tgtgttcctt cacgaacagc gtgtgcatca gttccagacc
     5521 tacccaccag ttcttgttga agccggtgat cggcagaccc gattgtgcgt ccatcggcag
     5581 gcgaccgttc accaggatca tcttgccatc ctggaacgtg cgcagcttgt tctgttgcgc
     5641 ctggctgctg ccgtaaatct gcgagccatc ccaccatgca gtgttttcgt tcaggaatg
//
LOCUS       DASUEG010000700         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1912559, whole genome shotgun sequence.
ACCESSION   DASUEG010000700 DASUEG010000000
VERSION     DASUEG010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912559"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFX11_13200"
     CDS             <1..354
                     /locus_tag="VFX11_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX4939629.1"
                     /translation="QETIGAPRTQANLNLEWAVPGLSSQAEQGLTLTGQILHTGAQFA
                     DTGNQQKVPDWTRLDLGARHTTPLADDQRLILHARLENVAGKDYWASSGGYPGEGYLT
                     LGGPRTLLVSATLEF"
     gene            488..1270
                     /gene="modA"
                     /locus_tag="VFX11_13205"
     CDS             488..1270
                     /gene="modA"
                     /locus_tag="VFX11_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005305146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4939630.1"
                     /translation="MTVLNRRGWIRLFSAGFATFTLLFGLHAHAGEIRLFAAASLTDV
                     LTALGNQYQASHPAITIKKSFAASSVLARQIENGAPAQIFLSADQDWANYLEQRGLLL
                     PASRQDLLGNQLVLIAPVGKEITVTPDATFNLAGAFSGRFCTGDVASVPVGKYTKQAF
                     THLGWWDAIVPRLVNTEDVRTALAFVARGECTLGAVYRTDAILSDKVTVVATLPANSH
                     APIVYPGGLLKGANAEAAAFWEFLQSESVRPVFERFGFSVAQ"
     gene            1286..1981
                     /gene="modB"
                     /locus_tag="VFX11_13210"
     CDS             1286..1981
                     /gene="modB"
                     /locus_tag="VFX11_13210"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX4939631.1"
                     /translation="MLTDMEWQALALSAKVGVWATLVSLLPGLWCGWLLARKQFVGKP
                     VLEAILFLPMVLPPTVPGYLLLVLFGSQGLLGRWLLENFNIQLAFNWKGAVLASATIA
                     FPLMVQAARIAVQMIDTRIESAARTLGSSPAKVWLTVTLPLALPGILVGAIMVFSRSL
                     GEFGATITFAGNIMGETQTLPLAIYSATHQVDGNNMAMRLIAICLTFAFLSLLLSNLL
                     QRRAERWLGVRHA"
     gene            1974..>2353
                     /locus_tag="VFX11_13215"
     CDS             1974..>2353
                     /locus_tag="VFX11_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4939632.1"
                     /translation="MLELDFVVQRGDFHLQVQQNLQHPVTGILGPSGGGKSTLLGAVA
                     GLVKPQQGTITLDGRVLFDSRSRIHIPAHQRRIGLVFQDGQLLPHLSVRHNLLYGYRN
                     IPLAERRFTLESVVDLLEIGPLLER"
BASE COUNT          433 a          768 c          725 g          427 t
ORIGIN      
        1 caggaaacca tcggcgcgcc ccgcacccag gccaacctga atctggaatg ggccgtgccg
       61 ggactttcat cccaagcgga acagggcctg accctcaccg gccagatcct ccataccggc
      121 gcccagttcg ccgacaccgg caaccagcaa aaggtgccgg actggacccg gctggatctg
      181 ggtgcccgcc acaccacgcc actggcggac gaccaacgcc tgatcctgca tgcccggctg
      241 gaaaacgtgg ccggcaagga ttactgggcc tccagcggcg gctatcccgg tgagggctac
      301 ctgaccctgg gcgggccgcg cacgctgctg gtatccgcca cgctggaatt ctaggggtag
      361 ccgctcaacg ggtaacaaac agcgcattga cagcctcatc gatatataca acgatacata
      421 tcgaatgtac atgacgagag gatggcggcg acgggcgcgg ccatcggtag tccggggcgg
      481 cgcaaacatg acagtactca acagacgcgg gtggattcgc cttttttccg ccggttttgc
      541 taccttcacc ctgctgttcg gcctgcacgc ccacgccggc gaaatcagac tgttcgcggc
      601 cgccagcctc accgacgtgc tgacagcact gggcaaccag taccaggcca gccaccccgc
      661 catcaccatc aaaaaatcct tcgctgcctc ctcggtactg gcccgccaga tcgaaaacgg
      721 cgcgcccgcg cagatctttc tctcggcaga ccaggactgg gccaattacc tggaacagcg
      781 cggcctgctg ctgcccgcca gccgccagga tctgctgggc aatcagctgg tgctgatcgc
      841 accggtgggc aaggaaatca ccgtgacacc cgatgccaca ttcaatctgg ctggcgcatt
      901 ttccggccgc ttctgcaccg gcgacgtcgc atcggtgccc gtcggcaaat acacgaaaca
      961 ggccttcacg cacctgggct ggtgggacgc tatcgtgccg cgcctggtga acaccgagga
     1021 tgtgcgcact gctctggcat tcgtggcgcg cggcgaatgc acgctgggcg cggtgtacag
     1081 gacggatgcg atcctcagcg acaaagtgac cgtggtggcc acattgcccg ccaacagtca
     1141 cgcacccatc gtctatcccg gtggtttgct gaaaggcgcc aacgccgagg ccgcggcctt
     1201 ctgggaattc ctgcaaagtg aaagcgtccg gcccgtgttt gaacgcttcg gcttcagtgt
     1261 ggcgcaatag aaggagccga cccgcatgct gaccgacatg gaatggcaag cactggcact
     1321 gtcggcaaag gtaggcgtct gggccacgct ggtcagcctg ctgcccggac tctggtgcgg
     1381 ctggctgctg gcgcgcaagc agtttgtcgg caagccggtg ctggaagcca ttctgtttct
     1441 gcccatggtg ttaccgccca cggtgcccgg ctatttactg ctggtgctgt ttggatcgca
     1501 gggcctgctg gggcgctggc tgctggagaa tttcaatatc cagctggcgt tcaactggaa
     1561 aggcgcggtg ctggcgtcgg ccaccatcgc ctttccattg atggtgcagg ccgcccgcat
     1621 cgcggtgcag atgatcgaca cgcgaatcga aagcgcggcg cgcacgctgg gatcgagtcc
     1681 ggcgaaagtc tggctcaccg tgacactgcc gctggcgctg cccgggattc tggtgggcgc
     1741 catcatggtg ttcagccgct cgctcggtga attcggcgcc accatcacct ttgccggcaa
     1801 catcatgggc gaaacccaga ccctgccgct ggcgatctat tccgccaccc atcaggtgga
     1861 cggcaacaac atggcgatgc gcctgatcgc gatctgcctg accttcgcgt ttttatcgct
     1921 gctgctcagc aatctgctgc aacggcgcgc ggaacgctgg ctgggggtac gtcatgcttg
     1981 aactggattt tgtcgtgcag cgcggcgatt ttcacctgca ggtgcaacag aatctgcagc
     2041 atccggtgac cggcattctg ggcccgtccg gcggcggcaa gagcacgtta ctgggcgccg
     2101 tggccgggct ggtgaaaccg caacagggca ccatcacact ggatggcagg gtgctgttcg
     2161 acagccgcag ccgcatccac attcccgccc accagcgtcg catcggcctg gtatttcagg
     2221 acggccagct gctgccccac ctgtcggtgc gccacaacct gctgtacggc tatcgcaaca
     2281 ttccgctggc ggagcggcgc ttcaccctgg aatccgtggt ggacctgctg gaaatcgggc
     2341 cgctgctgga acg
//
LOCUS       DASUEG010000701         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_974931, whole genome shotgun sequence.
ACCESSION   DASUEG010000701 DASUEG010000000
VERSION     DASUEG010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974931"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1295)
                     /locus_tag="VFX11_13220"
     CDS             complement(27..1295)
                     /locus_tag="VFX11_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4939633.1"
                     /translation="MGERGLAYKLGLLFCLYFSQGLPFGLFSHAVPTLLRSYGVDLEI
                     IGLVSMLGLPWALKFLWAPWVDRYHFPRLGQRKSWILPLQVSAVLVLVGFTLLDPAQL
                     KQGSWLYLFILLGLINLIAATQDIATDGLAVQALRPQERGLANGLQVGGYRVGMLFGG
                     GLVLVILDYLGWQNCFYVLALVLLLVTIPVLFYREEASAGQMAPDHVKNHVQNHARPA
                     WSAIVSFAMQPGMWAWLVLLMLYKVGDSFGSAMGKPLLVDLGLTLEQIGWIGGGVGMG
                     AGILGAVAGGWWVQTLGRVRALVLFGVLQSVALLGYAWLAWAGPGLVGVVAVNALEHF
                     AGGMATAALFTLMMDACRHEQAGSDYAVQASLQVSITGLAHALSGFSAKSLGYGWHFV
                     AAMAISLLALLLVRPWLQRLPQPQRAAWHA"
     gene            complement(1299..2360)
                     /gene="rlmM"
                     /locus_tag="VFX11_13225"
     CDS             complement(1299..2360)
                     /gene="rlmM"
                     /locus_tag="VFX11_13225"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEX4939634.1"
                     /translation="MQQVLVTCRAGLESDIAAEIQTKSAAVGVHGFVRAQAGDGYVIF
                     EGYQPDGGELLVQRLRFADLIFARQWLLVIAHLQALPPGDRITPIVQAYADAPAFSDC
                     WLTFPDTNEGKGLSRLTTKLEKPLGGALKKIQAKNADSAPWIAHVFFVTGDDMWVGIT
                     PRRNAAPWATGYPRLRQPPSAPSRSTLKLEEAWLQLIPEHLRSIYIKPFQKAVDLGAA
                     PGGWTWQLVHRGLQVVAVDNGPMDKQLMDSGQVTHLREDAFAYKPPRQVDWLVCDMVE
                     KPARVAELMERWLTKGWCRFAIFNLKLPMKKRWQEVDQILSRVREELDAAGIEYRLAA
                     RQLYHDREEITVFVGPKES"
     gene            2547..>4218
                     /locus_tag="VFX11_13230"
     CDS             2547..>4218
                     /locus_tag="VFX11_13230"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX4939635.1"
                     /translation="MTSFSEILAMAKVLAKAVKARPQPRDTRMSLGLMVEQNAAKTPD
                     ALMCLFEGKTITWGQFNFLANRMANQLRQMGVKKGDVVAVMMDNRIENLAAVTALTKL
                     GAAASMLNTHLTDEPLVHCINTINSRKIVVGEEWLASIDSIRRQLPFSSPNDFLYVRD
                     QGQTRCPDWATPCRPDDFAADNPPETRTIQLKDLAYYIFTSGTTGLPKASVITHDRHC
                     RMSMAFGEIALDMQPSDRIYVCLPLYHSSAMFIGFGSITHAGASMFLRRKFSASNLLD
                     EARQYNTNCFAYIGELCRYLLLQPQKPDDASNPIKKCVGNGLRPDIWMEFKTRFGIEK
                     ICEFYGASEGNAGCVNAFNKDKTVGFCAVKHALVRYDVLNDELIRNKKGFCEKARKGE
                     PGLMLIEINDKAVFDGYANADATAKKVLQNVFKKGDRYFNTGDLLKQVDVGFAMGLPH
                     YQFVDRIGDTYRWKGENVSTNEIGEIINSAATVEYCNLYGVQLPGTDGRAGMAAIVPA
                     GDTLDLAELSRHILEKVPGYARPLFIRQIREMDTTGTFKMKKGDLREQA"
BASE COUNT          957 a         1444 c         1121 g          696 t
ORIGIN      
        1 ttcctgtttc tttcgagtat aggcgttcaa gcatgccagg ccgcgcgctg gggttgaggc
       61 aaacgctgca gccagggtcg caccagcaac agcgccagca ggctgatggc catggctgcc
      121 acgaagtgcc atccataacc cagcgacttg gcgctgaatc cgctcaaggc atgggccaga
      181 ccggtaatcg acacctgcaa actggcctgc accgcatagt cggacccggc ctgctcatga
      241 cggcaggcgt ccatcatcag ggtgaacagc gcggcggtgg ccatgccgcc ggcgaaatgc
      301 tccagcgcat tcaccgccac cacaccgaca agtccgggac cagcccaggc cagccaggca
      361 tagcccaaca aggcgaccga ctgcagcacg ccgaacagca ccagcgcccg cacccggccc
      421 agggtctgca cccaccagcc gcccgccacc gctcccagga tccccgcgcc catgcccacg
      481 ccgccgccaa tccagccaat ctgctccagc gtcaacccca gatccaccag cagcggcttg
      541 cccatggccg acccgaacga atcccccacc ttgtacagca tcagcagcac cagccaggcc
      601 cacataccgg gctgcatcgc aaagctcact atggccgacc aggccggccg cgcatggttc
      661 tgcacgtggt tcttcacatg gtccggcgcc atttgcccgg cggaggcttc ttcccggtaa
      721 aacagcaccg gaatggtgac cagcaacagc accagcgcca gcacgtagaa acagttttgc
      781 cagcccaggt aatccagaat caccagcacc agaccgccgc caaacagcat gccgacgcga
      841 tagccgccca cctgcagacc attggccaga cctcgctcct gcggccgcag cgcctgcacg
      901 gcaagtccat cggtagcgat atcctgcgtg gcggcgatca gattgatcag acccagcaga
      961 atgaacagat acagccagct gccctgtttc aactgcgccg ggtccagcag cgtgaaaccc
     1021 accagcacca gaacggcact gacctgcaac ggcagaatcc agcttttgcg ctggcccaga
     1081 cggggaaaat gatagcgatc cacccacggc gcccagagaa atttcagcgc ccacggcagg
     1141 cccagcatgg acaccagacc aatgatctcc agatccacgc cgtaactgcg cagcaacgtc
     1201 ggcacggcat ggctgaacag gccgaacggc aggccctggg aaaaatacag gcagaacagc
     1261 aagcccagtt tgtaggccag accccgctcc cccaccgttt acgactcctt tggccccaca
     1321 aacaccgtga tttcctcacg gtcatggtac agctggcgcg ccgccagccg gtattcaatg
     1381 ccggccgcgt ccagttcctc ccgcacccgc gacagaatct ggtccacctc ctgccagcgc
     1441 tttttcatcg gcagtttcag gttgaaaatg gcgaaacggc accagccctt ggtcagccag
     1501 cgctccatca gttccgccac ccgggccggt ttctccacca tgtcacatac cagccagtcc
     1561 acctgccggg gcggcttata ggcaaaggca tcttccctca aatgggtaac ctggccgctg
     1621 tccatcagct gcttgtccat ggggccgttg tccaccgcca ctacctgcag accgcgatgc
     1681 accagctgcc aggtccagcc accgggagcc gcacccagat ccaccgcttt ctggaacggt
     1741 ttgatgtaga tactgcgcag atgctcggga atcagctgca gccaggcttc ctccagcttc
     1801 agcgtggaac ggctgggggc cgacggcggc tgacgcaaac gcgggtaacc agtagcccag
     1861 ggcgcggcat tccggcgcgg cgtgatgccc acccacatgt catcgccggt gacgaaaaag
     1921 acatgggcga tccagggcgc ggaatcggcg tttttggcct ggattttttt cagggcgccg
     1981 cccagcggct tctccagctt ggtagtcagg cgcgacagcc ccttgccctc gttggtatcg
     2041 ggaaacgtca gccagcaatc gctgaacgct ggcgcatcgg cataggcctg cacaatcggc
     2101 gtaatgcggt cgccgggcgg caacgcctgc agatgcgcaa tcaccagcaa ccactgacgg
     2161 gcaaaaatca gatccgcaaa ccgcagccgc tgcaccagca gttcgccgcc gtcaggctgg
     2221 tagccttcga aaatcacgta accatcaccc gcctgcgcgc gcacgaaacc atgcaccccc
     2281 acggcggcag acttggtctg gatctcggcg gcgatatcgg attccaggcc ggcacggcag
     2341 gtgacaagga cttgttgcat gaggagggat tcactgcagt gtggggaaag gatgcgcgca
     2401 ttctacccaa acaccctgac cgcgcctacc aatcacgctt gccatccatt tgacacaaat
     2461 tcccggttgc gtcccggaac aggttcggta tatatagccc tcgaccaatc agtcacacac
     2521 cgattcagta gcacgggcct attcccatga cctcattttc tgaaatcctc gccatggcca
     2581 aagtactggc caaagccgtc aaggccagac cccagccccg cgacacccgg atgtccctgg
     2641 gcctgatggt ggaacagaac gccgccaaaa cgcccgatgc cctcatgtgc ctgttcgagg
     2701 gcaaaaccat cacctggggc cagttcaatt tcctggccaa ccgcatggca aaccagctcc
     2761 ggcaaatggg cgtcaaaaaa ggcgatgtgg tggctgtgat gatggacaac cgcatcgaaa
     2821 acctggcggc cgtcactgcg ctcaccaaac tgggcgcggc agcgtccatg ctcaacacgc
     2881 acctcaccga cgaaccgctg gtgcactgca tcaacacgat caactcacgc aagattgtcg
     2941 tcggcgagga gtggctggca tccatcgatt ccattcgccg ccagcttccc ttttccagtc
     3001 cgaacgattt cctgtacgtg cgcgaccagg gccaaacacg ctgccccgac tgggccaccc
     3061 cgtgccgccc cgatgatttt gctgccgaca atccgccgga aacgcgaacc atccagctca
     3121 aggatctggc ctactacatc ttcacctcgg gcaccaccgg cctgccgaaa gcctccgtga
     3181 tcacccatga ccgccattgc cgcatgagca tggccttcgg cgaaatcgcg ctggacatgc
     3241 aaccgtccga ccgcatctat gtctgcctgc cgctctacca ttccagcgcc atgttcatcg
     3301 gcttcggttc catcacccat gctggcgcct ccatgttcct gcgccgcaaa ttttccgcca
     3361 gcaatctgct cgacgaagcg cgccagtaca acaccaactg tttcgcctac atcggcgaac
     3421 tctgccgcta ccttttgctg caaccgcaaa aaccggacga cgcgagtaac cccatcaaaa
     3481 aatgcgtcgg caacggcctg cgccccgaca tctggatgga attcaaaacc cgcttcggca
     3541 ttgaaaaaat ctgcgaattc tacggcgcca gcgaaggcaa cgccggctgc gtcaatgcct
     3601 tcaacaagga caagaccgtt ggtttctgcg ccgtaaaaca cgcgctggta cgctacgacg
     3661 tgctgaacga cgaactcata cgcaacaaaa aaggtttctg cgaaaaagcc aggaaaggcg
     3721 agccgggact gatgctgatc gaaatcaacg acaaggcggt tttcgatggt tacgccaatg
     3781 ccgacgccac cgcgaaaaaa gtgctgcaga atgtgttcaa gaaaggcgac cgctacttca
     3841 ataccggcga tctgctgaaa caggtggacg tgggttttgc gatgggcctg ccccactatc
     3901 agttcgtaga ccgcatcggc gacacctacc gctggaaagg cgaaaacgtc tccaccaacg
     3961 agatcggcga gatcatcaac agtgccgcca ccgttgagta ctgcaatctg tacggcgtgc
     4021 aactgccagg caccgacgga cgtgccggca tggccgccat cgtaccggcg ggcgacactc
     4081 tggatctggc ggaactgtca cgccacatcc tggaaaaagt gcccggctac gcgcggccgc
     4141 tgttcatccg ccagatccgc gaaatggaca cgaccggcac attcaaaatg aaaaagggtg
     4201 atctgcggga acaggcgt
//
LOCUS       DASUEG010000702         9560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1602178, whole genome shotgun sequence.
ACCESSION   DASUEG010000702 DASUEG010000000
VERSION     DASUEG010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602178"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFX11_13235"
     CDS             complement(<1..912)
                     /locus_tag="VFX11_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4939636.1"
                     /translation="MSLVYEAYNRSSLKQPPKTDFIGKWRVEYLAFSEPENAHKVPLI
                     VLGGAFQNFNSYKYCVEEILKAVPVILVDLPSLGNNDQLAPQLGMEDLADLLFLWTTK
                     VGLEKVSLMGLSLGSVLASTYAYKQPQVMHKLIVTGILVRPRKSWRMLLEESVKVLDE
                     GRLDEFGQAVVLYLVNHARLKETEITDITRKLFHRQMRSFHDNEQQRYRINAKRLTEV
                     ESILGYPECDTLVATGEYDSFTLPWENAQFAARCPNATFALINGADHVAQLEKRDESV
                     EMFATFLRDGDLKAVKGIQVFGRGEYEQ"
     gene            1160..2395
                     /locus_tag="VFX11_13240"
     CDS             1160..2395
                     /locus_tag="VFX11_13240"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX4939637.1"
                     /translation="MQRLGRAAREASRATARATTGTKNAALAAIHEALQASKADILAA
                     NAQDMEAGRARGLDAAMLDRLAIGEKTFAGMLEGLRQVMDLADPVGAITDLVYRPTGI
                     QVGKMRVPLGVIGIIYESRPNVTLEAAALCLKSGNATILRGGSEAIHSNKAIAACIQQ
                     GLEKAGLPREAVQVVETTDRAAVSALITMPEFVDVIVPRGGKGLIERISGEARVPVIY
                     HLDGNCHTFIERDADMAKAIPVALNAKTHRYGTCNTMETLLVDAPVAEAALKALAPLY
                     REKGVELRGCEKTQALIEANPATEEDWYTEYLAPVLAIKVVDGLDEAIAHINKYGSHH
                     SDAIITESVTKARRFLNEVDSSSVMVNASTRFADGFEYGLGAEIGISTNKIHARGPVG
                     LEGLTSQKWVVLGDGHIRQ"
     gene            complement(2529..3323)
                     /locus_tag="VFX11_13245"
     CDS             complement(2529..3323)
                     /locus_tag="VFX11_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4939638.1"
                     /translation="MMIDGLSFILAGALVGFCVGLTGVGGGSLMTPLLTMMGVPLHTA
                     IGTDLLYASITKSSGAVVHARKKNINWPVVLTLSAGSIPAALLTLWALKAFFGNANEY
                     KDILTVSLGFMLMLTAASLIFRKQLQRLHDANPLQNHIRQIIDRHTRTFTLLMGLALG
                     VLVTLSSVGAGAFGVVVLLSLYPRLSTIEIIGTDVTHAVLLTLVAGLGHLQMGNVDMH
                     LLGWLLLGSIPAIIAGTHLSSRMPDDVIRPILGGTLALLSVKFVFY"
     gene            3516..4181
                     /gene="nadD"
                     /locus_tag="VFX11_13250"
     CDS             3516..4181
                     /gene="nadD"
                     /locus_tag="VFX11_13250"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX4939639.1"
                     /translation="MTSSPPALTGLYGGSFDPVHIGHVATAEELLVRLPFREIRLLPA
                     ARSPLKDRSLANHHRVAMLKLAVQGHAGLAVDDRELRRAPPSFTVDTLHEVRSELGRA
                     APLAFIMGLDSFLELPRWKDWQHLTDYAHLVVVSRPGSHPSFAPPLADWLEKHRITEP
                     ESLESRPFGGVLLVETTPHDVASRVIRAAVRAGQSTADWLAPAVRDYIDTHHLYAGDA
                     DPQ"
     gene            4178..4546
                     /gene="rsfS"
                     /locus_tag="VFX11_13255"
     CDS             4178..4546
                     /gene="rsfS"
                     /locus_tag="VFX11_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX4939640.1"
                     /translation="MTSLSTETQQLVDIAQKALEDLKAKDVLTLDVRSLTSLADVMII
                     ASGTSSRHVKSLADNVVKMVKEAGLKPLGMEGERDGDWILIDLAGVIVHVMQPATRAF
                     YDLERLWTPGASSHIAQAKA"
     gene            4550..5017
                     /gene="rlmH"
                     /locus_tag="VFX11_13260"
     CDS             4550..5017
                     /gene="rlmH"
                     /locus_tag="VFX11_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX4939641.1"
                     /translation="MKLRLLAVGTRMPDWVEDGFADYAKRLSGDISLELVEITAGKRL
                     KATDLARVKEQEGEALLAAIKPQERVIALDVLGRQLSTEDMAATLRDWHVDGRPAALL
                     IGGPEGLSRAALDRADEKWSLSRLTLPHPLVRIVIAEQLYRAWSLLKGHPYHR"
     gene            5110..6999
                     /gene="mrdA"
                     /locus_tag="VFX11_13265"
     CDS             5110..6999
                     /gene="mrdA"
                     /locus_tag="VFX11_13265"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4939642.1"
                     /translation="MRRSTSITIKDNQQEAALFRRRAAVAAAFVVVMFGVLIGRYYFL
                     QVVEHETYTVLSENNRVHLEAIAPPRGFIYDRKGRLLADNQPTFSLTINRQQAEDVDA
                     LIKQLTPVLQLTEEDIRRFKARAKVARKYEEVPLRLRLSEEDIARFSEVKYDLKGANV
                     NVELSRYYPHSDLFSHAIGYVSRISEKEEKELDPVKYAGTNLIGKIGIEKYYENYLHG
                     TVGYQHVETNAHGRLIRVLKRTPPVRGSDLTLHLDYDLQKIAHDQLAGRRGAVVALDP
                     NTGGVLAFVSNPTFDPNAFVGGIPYKLYSHYRDHIDKPLYNRALQGVYPPGSTIKPFE
                     AMGGLHYGLVNWDYRIGDPGFFSLPGDSHRFRDWKKNGHGVVDMHKAVVESCDTYFYQ
                     LSDRMGVDRFHDWMKHFGFGAKTGIDLVNEKAATLPSVKWKRERLKAPWYRGEMMSVG
                     IGQGYFTATPLQLAMATAILANGGNHVRPHLLKSASGTEDFSNINQSDYKVPYAGDAA
                     DWERMKLSMRDVVHGPGGTARATGYKVQGYEMAGKTGTAQVKGIKQGEKYDESRIDER
                     HWDHGWFIGFAPVDNPKIAVAVLVENGKHGSSVAPIAKALFDYELLGKVPEAPVLPTH
                     EASDE"
     gene            6999..8153
                     /gene="rodA"
                     /locus_tag="VFX11_13270"
     CDS             6999..8153
                     /gene="rodA"
                     /locus_tag="VFX11_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX4939643.1"
                     /translation="MMSTTQDRFLRHMPRDGSQFKRKFDLWGMLHIDLPLLIALLLAA
                     SLGLFELYSASGRHLDLTLKQLMSFGIGLTVMFVLAQIPPRTYQTLSPWFYAGGVVLL
                     VAVAVVGDVRMGAQRWLSIPGFGSVQPSEFMKLGMPMMLAWFLAARVLPPSVGTVGIA
                     LVLIGIPALLVAEQPDLGTSILVAAAGLFVLFLAGLPWWLIGGFAAAMAPLVWVAWNY
                     LLHDYQRQRVMTLIDPEADPLGHGWNIIQSKTAIGSGGITGKGWMNGSQSHLEFLPEG
                     HTDFIIAAYSEEFGLIGVLILFSIYLFIIGRSLYIASTAQDSYSRLLAGAITLSFFVY
                     VFVNAGMVAGILPVVGVPLPFISYGGTAIITLCSGFGILMSIQTHKKLIS"
     gene            8193..9257
                     /gene="mltB"
                     /locus_tag="VFX11_13275"
     CDS             8193..9257
                     /gene="mltB"
                     /locus_tag="VFX11_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX4939644.1"
                     /translation="MLKTLLCALISSACLKPVPANAVGEFVKYEELDAIIAHLEQEKI
                     YAPGELTSLFEQVARDEGVLKAIARPAEGTKEWKEYRPIFLTQERINKGLAFWREHEA
                     VLNRAEAQYGVPPEMILAILGVETKYGGNKGRNRVIDALATLGLDYPPRAPFFRKELR
                     EFLILSRENGLDPLNTWGSYAGAMGFPQFMPSSWRRLAVDFDGDSKIDLINNPVDAIG
                     SIANYFKANGWKTGEPVTVRARIINQEYDSIVSKELKTVSTLGEIAKKGLVPREAGSH
                     LATTPASAIRLQGENGGEFWLGFTNFYVITTYNRSILYAMAAFQLSQELKAAREAELA
                     AAAAQADATATLPADVTPAP"
     gene            9281..>9560
                     /locus_tag="VFX11_13280"
     CDS             9281..>9560
                     /locus_tag="VFX11_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016914098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939645.1"
                     /translation="MTGNRLLTVCASALLLTACVTTPPPARKPVNPITRLPPQTQPVP
                     NPADRPAFGTKAFNVIKDAGPDAHVDVSQVPDAEPVEEPLSAYGNRNPY"
BASE COUNT         1942 a         3073 c         2746 g         1799 t
ORIGIN      
        1 ctgctcgtac tcgccacgcc cgaatacctg aatgcccttt accgccttca gatcaccgtc
       61 acgcaggaag gtggcaaaca tttccaccga ctcgtcacgc ttctccagct gggccacatg
      121 gtccgcacca ttgatcaggg caaacgtggc attcgggcag cgcgcggcaa attgcgcgtt
      181 ctcccatggc aaggtgaagc tgtcgtactc acccgttgcc accagcgtgt cacactcggg
      241 atatccgagg atggactcca cttcggtcag ccgtttggcg ttgatgcggt aacgctgctg
      301 ctcgttgtcg tggaagctgc gcatctggcg atggaacagc ttgcgcgtga tgtcggtgat
      361 ctcggtttcc ttgaggcggg catggttcac cagatacagc accacggcct gaccaaactc
      421 gtcgaggcga ccttcgtcca gcaccttgac cgactcttcc agcagcatgc gccaggactt
      481 gcgcggccgc acgaggatgc cggtgacaat cagcttgtgc atgacctgcg gctgcttgta
      541 ggcataggtc gacgccagca cagaccccag cgacaacccc atcagggaaa ccttttccag
      601 ccccaccttt gtggtccaga ggaaaagcag gtcggccaga tcttccatgc caagctgtgg
      661 cgccagctgg tcgttattgc ccagtgacgg caggtccacc aggatcactg gcaccgcctt
      721 caggatttcc tccacacagt acttgtagga gttgaaattc tggaaagcgc cgcccagcac
      781 gatcagcggc accttgtggg cattctccgg ttcgctgaag gccagatact cgacccgcca
      841 cttgccgatg aagtcggtct tgggcggctg cttcagcgag gaacggttat aggcctcgta
      901 aactaaggac atgggggccc ctgtctgtca ttcattcttg ttggttttgc cctccggtgc
      961 actcatccag cctgccgggg gcatttcttc ttgattggac gcatcctacc actggccaat
     1021 ggcacttgcg tagcagggat tcacctgtcg ttgtcccgga cggcaccctg ccgctgacga
     1081 ccgtaccgcc gtatacttca ccattcagac ccatgacgga attgcttcag tgaccgacac
     1141 ctccgacatt tcagcctaca tgcagcgcct gggccgtgcc gcccgcgagg cttcccgtgc
     1201 cacggcccgt gccaccaccg gcaccaagaa tgcagcactg gccgccatcc acgaggccct
     1261 gcaggccagc aaggccgaca tcctggctgc caacgcgcag gacatggagg ccggccgtgc
     1321 ccgtggactc gatgccgcca tgctggatcg tctggccatc ggcgagaaaa cctttgccgg
     1381 catgctggaa ggcctgcgcc aagtgatgga cctcgccgac cctgtcggcg ccatcaccga
     1441 tctggtctac cgtcctaccg gcattcaggt gggcaaaatg cgcgtgccgc tgggcgttat
     1501 cggcatcatc tacgagtccc gccccaacgt gacgctggag gcggcggcgc tgtgcctgaa
     1561 gtcgggcaat gccaccatcc tgcgcggcgg ttccgaagcc atccactcca acaaggccat
     1621 tgccgcctgc atccagcaag gactggaaaa ggccggcctg ccccgtgaag ccgttcaggt
     1681 cgtggaaacc accgaccgtg cggccgtatc agccctcatt acgatgcccg aattcgtgga
     1741 cgtgatcgtg ccccgcggcg gcaagggact gatcgagcgt atctcgggcg aagcccgcgt
     1801 gccggtgatc taccatctcg atggcaactg ccacacgttc atcgagcgtg acgccgacat
     1861 ggccaaggcc attccggtag ccctgaacgc caagacgcac cgctacggca cctgcaacac
     1921 catggaaacc ctgctagtgg atgcgcccgt ggccgaagcg gcccttaagg cactggcccc
     1981 gctctatcgc gagaagggcg tggagctgcg cggttgcgag aagacccaag ccctcatcga
     2041 ggccaacccc gccaccgaag aggactggta caccgagtac ctggccccgg tactggccat
     2101 caaggtggtc gacggactgg acgaggccat cgcccatatc aacaaatacg gttcgcacca
     2161 ttccgacgcc atcatcaccg aaagcgtgac caaggcccgg cgcttcctga acgaagtgga
     2221 ctccagctcg gtcatggtga atgcctcaac ccgttttgcc gacggctttg agtacggtct
     2281 aggtgcagaa atcggcattt ccaccaacaa gatccatgcc cgcggcccgg tggggctgga
     2341 aggcctgacc agccagaagt gggtggtgct gggcgacggc catatccggc aataactcct
     2401 cccgcggcaa tacccgcacg caggctgcaa cagatcaccg gcgcagcctc agtgcgggta
     2461 ttcaggagac aagaccgttc aggcaaccaa ctgacgttag tcagactgtc tatcgtgaaa
     2521 gacgactctc aatagaacac gaacttcaca ctgagcaacg ccagtgtgcc cccgaggatg
     2581 gggcggatga cgtcgtccgg catgcgggac gacagatggg taccggcgat gatggcaggg
     2641 atggagccca gcaacagcca acccagcaga tgcatgtcga cattccccat ctgcaaatga
     2701 ccaagaccgg cgaccaaggt caacagtaca gcgtgcgtga catctgtccc gatgatctca
     2761 atggtgctga ggcggggata caaactgagc agcaccacca caccaaacgc ccccgcgcct
     2821 accgacgaca gcgtgaccag cacgcccaac gccaatccca tgagcagcgt aaacgtcctg
     2881 gtatgccggt cgatgatctg acggatgtgg ttttgcagcg gattggcatc gtgcagcctc
     2941 tgcaattgct tgcgaaagat cagtgaagcg gcggtcagca tcagcatgaa gcccagcgac
     3001 accgtgagga tgtccttgta ctcgttggca ttcccgaaga atgccttcaa tgcccacaag
     3061 gtcagcaagg cggctggaat gctgcctgca gacagggtca acaccactgg ccaattgatg
     3121 ttcttcttcc gggcatgtac cactgcgccg ctggatttgg tgatggaggc ataaagcagg
     3181 tcagtaccga tggccgtatg cagcggcacc cccatcatgg tcagcagcgg cgtcatcaga
     3241 gaaccaccac ccacccctgt gaggcccacg cagaagccga ccaatgcgcc tgccagaatg
     3301 aaactcaagc cgtcaatcat cataaggttc atcaaaaagc aggttatgga acaccaatcg
     3361 gaacagcact ccggtcgccc ggcactctac gacggcaaag catattctcc tatatatcgt
     3421 ttcgaaatac agttataacc ataagagata tatcttccgc cacgatcacc cggtggcgct
     3481 cggcagccgc ccgccagtcc cgtacaatcg ccaccatgac ctcatccccc cctgctctta
     3541 ccggcctgta tggtggttct ttcgatccgg tccacatcgg ccatgtagca acggcggagg
     3601 aactgctggt gcggctgcct tttcgggaaa tccgcctgct acccgccgcg cgctcaccgc
     3661 tgaaggacag gtcgctggcc aaccatcacc gtgtcgccat gctcaaactg gcggtacagg
     3721 gccatgccgg gctggcagtg gacgaccgcg aactgcgccg cgcccccccg tcctttaccg
     3781 tcgacacctt gcacgaggta cgcagcgagc tgggccgggc cgccccgctg gccttcatca
     3841 tgggactgga cagctttctg gagctgcccc gctggaaaga ttggcagcac ctgacggatt
     3901 acgcccatct ggtagtggtc agccgccctg gcagtcaccc gagctttgcc cccccactgg
     3961 ccgactggct ggaaaaacat cgaattaccg agccggaatc gctagaatcg cgcccctttg
     4021 gtggtgtgtt gctggtggag acgactccgc atgatgtagc cagccgtgtc atccgggccg
     4081 ccgtccgggc aggtcagtcc accgcagact ggcttgctcc tgccgtccgg gactatattg
     4141 acacccatca cctctatgcc ggagatgcag acccccaatg acttccctgt ccaccgaaac
     4201 acagcagctc gtcgacattg cccaaaaggc actggaagac ctcaaggcca aagacgtgct
     4261 gaccctcgat gttcgctccc tcaccagcct cgccgatgtc atgatcattg cctccggtac
     4321 ctccagccgt catgtgaagt cgctggccga caacgtcgtg aaaatggtga aggaagccgg
     4381 cctcaagccg ctgggcatgg aaggcgagcg tgatggcgac tggatcctga tcgacctggc
     4441 cggagtcatc gtgcatgtca tgcagcctgc cacccgcgcg ttctacgatc tcgagcgcct
     4501 ctggaccccc ggggccagca gccatatcgc ccaagccaaa gcctgattca tgaagctgcg
     4561 tctgcttgcc gtgggcaccc gcatgcccga ctgggtggaa gacggatttg ccgactacgc
     4621 caaacgcctt tccggtgaca tcagcctgga gctggtggaa atcacggcgg ggaagcggct
     4681 gaaggccact gacctggccc gcgtgaagga gcaggagggg gaagccctgc tggcggccat
     4741 caagccgcag gagcgggtga ttgccctgga tgtattgggc cgccagctgt ctaccgaaga
     4801 tatggctgcc accctgcgtg actggcatgt ggatggccgc cctgccgccc tgctgatcgg
     4861 tggtccggaa ggactgagcc gtgccgccct ggaccgggcc gatgagaaat ggtccctgtc
     4921 acgcctgacc ctgccgcacc cgctggtgcg catcgtgatt gccgaacagc tgtaccgtgc
     4981 ctggagcctg ctcaagggac acccctacca ccggtgattc gccccgcgtg cgatgatctt
     5041 caacaaaaac ctgctggctt ttaccgtcag ccctgcctga aaatcgcgtt ttcgacagac
     5101 tcctgagcca tgcgccgatc cacctccatc accatcaagg acaaccagca ggaagccgcg
     5161 cttttccggc gccgcgccgc cgtggccgcc gctttcgtgg tggtcatgtt tggcgtactg
     5221 atcggccgtt actatttcct gcaggtcgtc gagcacgaaa cctataccgt gctctcggag
     5281 aacaaccgcg tacacctgga agccatcgcc cctccgcgcg gcttcatcta cgaccgcaag
     5341 ggacgtctgc tggccgacaa ccagcccacc ttctcgctca ccatcaaccg ccagcaggcg
     5401 gaggatgttg atgccctgat caaacagctg acgcctgtgc tccagctgac cgaagaagac
     5461 attcgccgct tcaaggcccg cgccaaggtt gcccgcaagt acgaggaagt accgctgcgc
     5521 ctgcgcctgt ccgaagagga cattgcgcgc ttctccgagg tcaaatacga cctgaaaggc
     5581 gccaacgtca atgtcgagct gtcacgctat taccctcaca gcgatctgtt ctcccatgcc
     5641 atcggctatg tctcccgcat ctccgaaaag gaagaaaagg aactggaccc ggtgaaatat
     5701 gccggcacca acctgatcgg caagatcggc atcgagaagt attacgagaa ttacctgcat
     5761 ggcacagtgg gctaccagca tgtggaaacc aatgcccacg gccgcctgat ccgcgtgttg
     5821 aagcgcaccc cgcccgttcg cggcagtgac ctgacgctgc atctggacta tgacctgcag
     5881 aaaattgccc acgaccaact ggcgggtcgt cgtggtgccg tcgtggccct cgatccgaac
     5941 accggtggcg tactggcctt tgtcagcaat ccgaccttcg atcccaacgc ctttgtgggg
     6001 ggtatcccct acaagcttta ctcacactat cgcgaccata tcgacaagcc gctctacaac
     6061 cgcgccctgc agggtgttta tccgcctggc tcgaccatca aaccgttcga ggccatgggg
     6121 gggctgcact atggtctggt gaactgggac taccgtattg gcgaccccgg cttcttcagt
     6181 ctgcccggcg actctcaccg ctttcgtgac tggaagaaga acggacacgg agtggtggac
     6241 atgcacaagg ctgtcgttga gtcctgcgac acgtattttt accagctctc cgatcgcatg
     6301 ggcgtcgacc gcttccatga ctggatgaag catttcggct ttggcgccaa aaccggcatt
     6361 gatctggtga acgagaaagc ggcaacgctg ccgtcggtga aatggaaacg tgagcgtctg
     6421 aaggcaccct ggtatcgcgg cgaaatgatg tccgtcggaa ttggccaagg ctacttcacc
     6481 gccacgccgc tgcagctggc catggctacg gccattctgg cgaacggcgg caatcacgtt
     6541 cgcccgcact tgctgaaaag cgcctcgggt accgaggatt tcagcaacat caaccagtcg
     6601 gattacaagg tcccctatgc cggtgacgca gcggactggg agcgcatgaa gctttccatg
     6661 cgtgacgtgg tacacggccc cggcggtacg gcccgggcca ctggctacaa ggtgcaaggc
     6721 tacgaaatgg ccggcaagac cggcacggca caggtcaagg gcatcaagca gggcgaaaag
     6781 tacgacgaat cgcgcattga cgagcggcac tgggaccacg gctggttcat cgggtttgct
     6841 cctgtcgaca acccgaagat cgccgtcgcc gtgctggtgg agaacggcaa acacgggtcc
     6901 agcgtggcac cgattgccaa agccctcttc gactacgaac ttctcggcaa ggtgcccgag
     6961 gcaccggtct tgcccacgca cgaagcgagt gacgaataat catgagcacg acacaggacc
     7021 gctttctgcg ccacatgccg cgtgacggct cgcagttcaa gcgcaaattc gacttgtggg
     7081 gcatgctgca catcgacctg cccttgctga ttgccctgct gttggccgcc agcctggggc
     7141 tattcgagct gtactcggca tccggtcggc atctggatct cacgctcaaa cagctgatga
     7201 gcttcggcat cggtttgacc gtgatgttcg tgctggcaca aattccgccc cgtacctacc
     7261 agaccctgtc gccctggttc tatgcaggtg gcgtcgtatt gctggtggcc gtcgcggtcg
     7321 tgggtgacgt gcgtatggga gcgcagcgct ggctgtccat tcctggcttt ggcagcgtgc
     7381 aaccctccga attcatgaag ctgggcatgc caatgatgct ggcctggttc cttgctgcgc
     7441 gtgtcctgcc ccccagcgtg ggaacggtcg ggatcgccct ggtcctgatc ggcatcccgg
     7501 ccctgctggt ggcagagcaa cccgaccttg gcacgtccat tctggtggcc gctgccggcc
     7561 tgtttgtact cttcctggcc ggtctgccct ggtggctgat tggtggcttt gcagcggcca
     7621 tggcgcctct ggtctgggtg gcctggaatt acctgctgca tgattatcag cgccagcgcg
     7681 tcatgacgct gatcgatccg gaggccgacc cgctgggaca tggctggaac atcatccagt
     7741 ccaaaacggc catcggctct ggcggtatta cgggcaaggg ctggatgaac ggctcgcagt
     7801 cccatctgga attcctgccc gaggggcata cggacttcat cattgcggcc tactccgaag
     7861 agttcggcct gatcggcgtg ctgatcctgt tcagtatcta cctgttcatc atcggtcgca
     7921 gcctctacat tgcctcaacg gcccaggaca gctactcgcg cttgctggcc ggcgccatca
     7981 cgctgtcgtt ctttgtctac gtgttcgtca atgccggcat ggtcgccggc atcctgccgg
     8041 tggtaggggt cccgctgccc ttcatcagtt atggcggcac ggccatcatc accctgtgct
     8101 ctggctttgg catcctgatg tcgatccaga cccacaagaa actcatcagc taagacggct
     8161 aaccggaacg gattgccacg gaggactcct gcatgctgaa aaccctgctc tgcgccctga
     8221 tcagcagtgc ctgcctgaaa cctgtcccgg ccaatgccgt cggcgagttc gtcaaatatg
     8281 aggaactgga tgccatcatt gcccatctgg agcaggagaa gatctatgcc ccgggagagc
     8341 tgaccagcct gttcgagcag gtggctcgcg acgagggcgt gctgaaagcg attgcccgac
     8401 ccgcagaggg aaccaaggag tggaaggagt accgtccgat tttccttacg caggaacgca
     8461 tcaacaaagg actggccttc tggcgcgaac atgaagccgt cctgaatcgg gcggaagccc
     8521 agtatggcgt accgcctgaa atgatcctgg ccatcctcgg cgtggaaacc aagtacggcg
     8581 gcaacaaggg ccgaaaccgg gtgattgatg ccctggcgac cctgggtctc gactatccgc
     8641 cgcgtgcccc cttcttccgc aaggagctgc gtgagttcct catcctgtcg cgcgagaacg
     8701 gcctggaccc gctcaacacg tggggttctt atgccggtgc catgggcttt ccgcagttca
     8761 tgccatccag ctggcgccgc ctggccgtgg attttgacgg ggacagcaag atcgacctga
     8821 tcaacaaccc ggtggatgcc attggctcga ttgccaacta cttcaaggcc aacggctgga
     8881 agaccggcga accggtcacc gtccgtgccc gcatcatcaa ccaggagtac gacagcatcg
     8941 tcagcaagga actcaagacc gtcagcacac tgggtgagat tgccaaaaag ggcctggtgc
     9001 cgcgtgaagc cggcagccac ctcgcaacca ctcccgcctc tgccattcgt ctgcagggtg
     9061 agaacggagg ggaattctgg ctggggttca caaatttcta cgtgatcacc acgtacaatc
     9121 gaagcatcct gtatgccatg gctgccttcc agctaagcca ggaactgaaa gccgcccgcg
     9181 aggcggaact ggctgcagcc gctgcccagg ctgacgccac tgcaacgttg ccggccgacg
     9241 taacgccagc gccctgacca ccggacaacc ggagccagag atgaccggaa accgcctgct
     9301 gaccgtatgt gcctccgccc tgctgctgac ggcctgcgtg accaccccgc caccggccag
     9361 gaagccggta aaccccatta cccggctgcc gccacagacg cagccggtgc cgaaccctgc
     9421 agatcgcccg gcctttggca ccaaagcgtt caatgtcatc aaggatgccg gccccgacgc
     9481 ccacgtggat gtgagccagg ttcctgatgc cgaaccggtg gaagagccgc tgagcgccta
     9541 tggcaatcgc aatccctata
//
LOCUS       DASUEG010000703         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1510662, whole genome shotgun sequence.
ACCESSION   DASUEG010000703 DASUEG010000000
VERSION     DASUEG010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510662"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>2236
                     /locus_tag="VFX11_13285"
     CDS             30..>2236
                     /locus_tag="VFX11_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4939646.1"
                     /translation="MWLTLEGDRTLQQLNQYARIVIKKQLENIDGVGEVQIAGRRDRT
                     IRVNLNVETMNAYGVTTQDLLQAFANEHFQMPGGFLVGGSTENLIKLDLEFHTPADLE
                     ALIVSYRKGAPIRLADIATIEDGLEDDRRLARYNDQQAVGLGIVKVSGSNAVAIIQEV
                     ERRLAQDIIPQLPPGMKLNIASNDADLIEELVSALEEHLVLGAFLTALVVWLFLRSLR
                     ATAIIAVSIPVSLMGSVALMYFAGFSFDTMTMLALLLLIGIVVDDAIVVLENIYRHRE
                     EVDPDPKSAALNGANQVVFAVMAATLTLVSIFVPVIFMEGIMGQFFSSFAVVVTGGVL
                     ISLFVSLTLTPMLCSRYLKVTPQHGAVYKFLENGFQRIEEIYRDLVGSSLRFRWMVVA
                     LTIVSVLPIGYFFGNIGKGFFPEEDESTFLIGFKTPLGASLDYTNERLTEIENVLKRH
                     REVAGYFSTIGTGETGQVNQGSIFVALKPADDRDDHLQVILARLRTDLAQIPGVKAFP
                     TPVSGAGNARGEPLQFVVMGPDIAGVAVQSHRLLNKLATIPELGQVDLDLQLELPQLT
                     LDVDRTRAADAGLSTRDISLAVNVLAGGVDVAKYNDLPGDGERYDVRLKAQEKSFRAL
                     DDLHRIYLRGRGGELVRLDTVVSVKEEIGPAAVTRFDLRYSGNFFSSPIVPLGDGVNL
                     VLAAAEDILPPGYTIQMVGQADEFGRTASYMATAFGIAIILVYMVLASQFDSF"
BASE COUNT          428 a          596 c          684 g          528 t
ORIGIN      
        1 ccaagcttgt ttttggtgca attcctgtaa tgtggctgac cctggaaggc gaccgcacct
       61 tgcagcagct taaccagtac gcccgtatcg tcatcaagaa gcagctggaa aacattgatg
      121 gcgtgggaga ggtgcagatc gctggcagac gggatcgcac cattcgcgtc aatctgaatg
      181 tcgaaacgat gaatgcctat ggcgtcacaa cacaggatct gctgcaggcc ttcgccaatg
      241 agcattttca gatgccgggc ggatttctgg tggggggcag caccgaaaat ctgatcaagc
      301 tggatctgga atttcatacc cccgccgatc ttgaggcact gattgtttcc tatcgaaagg
      361 gcgcgcccat ccggcttgcg gatatcgcca caatcgaaga tggcctggag gatgatcgcc
      421 gtctggcgcg ttacaatgat cagcaggcgg tgggccttgg cattgtcaag gtttcgggtt
      481 caaacgcagt ggcgatcatc caggaggtgg agcgccggct tgcgcaggac atcattccac
      541 aacttccgcc ggggatgaaa ctgaacattg cgtccaatga cgcggatctg attgaagaac
      601 tggtgtccgc gctggaagaa catctggtgt tgggcgcttt tcttacagcg ctggtggtct
      661 ggctgtttct cagaagcctg cgcgcaacgg cgattatcgc ggtgtcgata ccggtttctc
      721 tgatgggttc ggtggcgctg atgtattttg ccggtttcag cttcgacacc atgacgatgc
      781 tggcgctgtt gctgctgatc ggcattgtgg tggatgacgc catcgtggtg ctggaaaaca
      841 tctaccggca ccgcgaagag gtcgatcctg atcccaaaag cgcggcgttg aatggcgcca
      901 accaggtcgt cttcgcggtc atggccgcga cgctcacgct ggtgtcgatc tttgtccccg
      961 tgatcttcat ggaaggcatc atgggacagt ttttcagctc gttcgcggtg gttgtcaccg
     1021 gcggtgtgct gatttctctt tttgtctctt tgacgctgac ccccatgctg tgctcgcgtt
     1081 atctcaaggt caccccccag cacggggcgg tgtataagtt tctggaaaac ggatttcagc
     1141 gcatcgagga gatctatcgc gatctggtgg gtagctcgct gcggttccgc tggatggtgg
     1201 tggcgctcac tatcgtttca gttctgccga tcggttattt cttcggaaat atcggcaagg
     1261 ggtttttccc ggaagaggat gaaagcacct ttctcatcgg tttcaaaacg ccgcttgggg
     1321 ccagcctcga ctataccaat gagcggctga cggagatcga gaacgtcctc aagcgccatc
     1381 gcgaagttgc cggctatttt tcgacgatcg gcaccggtga aaccggacag gtcaatcagg
     1441 gctcgatctt tgtggccctg aagccggccg acgatagaga cgaccatttg caggtaattc
     1501 tggcccggtt gcgcaccgat ctggcgcaaa taccgggtgt caaggcgttt cccacccccg
     1561 tttcgggggc gggcaatgcg cgcggtgagc cattgcagtt cgtcgtgatg ggccccgata
     1621 ttgcgggcgt cgcagttcag agccaccggt tgctgaacaa actggcgacc atccccgaac
     1681 tgggccaggt ggatctggat ttgcagcttg agctgcctca gcttaccctg gacgttgacc
     1741 gcacgcgcgc cgccgatgcc gggctttcga cccgggatat ttcgctggcg gtaaatgtac
     1801 tggcgggcgg cgtggacgtc gccaagtaca atgatctgcc cggcgacggc gagcgctacg
     1861 acgtacggct gaaagcccag gaaaaaagct ttcgcgccct tgacgacctg catcgtatct
     1921 atttgcgcgg gcggggcggc gagctggtgc gcctggatac ggttgtttcg gtcaaggaag
     1981 aaatcgggcc cgcagcagtt acccgtttcg atctgcggta ttcgggaaat ttcttcagtt
     2041 cccccatcgt tccgctgggc gatggcgtca acctggtgct ggcggcggcc gaagacatat
     2101 tgccgcccgg atacaccatc cagatggttg ggcaggccga cgaatttggc agaacggcct
     2161 cctatatggc gacggcattt ggaattgcga ttattctcgt ttacatggtg ctggccagcc
     2221 agttcgattc ctttat
//
LOCUS       DASUEG010000704         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_605023, whole genome shotgun sequence.
ACCESSION   DASUEG010000704 DASUEG010000000
VERSION     DASUEG010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605023"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFX11_13290"
     CDS             complement(<1..177)
                     /locus_tag="VFX11_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX4939647.1"
                     /translation="MSPAPYTGRFAPSPTGPLHLGSLLTALASFLDARARSGIWLVRM
                     EDLDPPREMAGAADD"
     gene            complement(253..>2006)
                     /locus_tag="VFX11_13295"
     CDS             complement(253..>2006)
                     /locus_tag="VFX11_13295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4939648.1"
                     /translation="RLLPAAACVLLVTLLAAQFIYAPQAMITLAKDAVATSAYASNLW
                     FAHIATDYLAADAVKSPLLHTWSLSVEEQFYFVWPLFVLLALRGRKGATPNRRRLLYA
                     MSGLAALSLLLSVLLTDIAQPWAFFSSPARAWEFAIGGLAFFVPVAWMAARPQLRTAC
                     FWAGLAALTYSATQFGHHTTFPGVAALLPVLGTSLLLISHAPGQTDLRTRLLHHPLLQ
                     WLGGRSYSWYLWHWPVLILAGMLLPEKHLGISMLCLVLSLLLADLSYRWVENPVRFHP
                     RLARQHGYALSLALCLTLASAGTAAWWRHAGTLEAASPTQIRFTQAMDDVPKTIYESN
                     CHLSLLAVETGDCFFGDPEATTTIALFGDSHAAQWFPALEKLARQQHWRLVSLTKSAC
                     PVAWLKPVNPQLGRPYDECSRWRDQVTEKIIALRPKVVVMGYYRYVSLPDAVQSTISE
                     RHWQDAMRETMQRFNDAGIAVAIMRDSPRPGFDVPTCLARQAKTPWLELDCDFSRTLA
                     LDSVEYNLNRQAAMDLEQVLFIDLSRRICASEICNPVDSDSGIVLFRDSHHLTTEFVR
                     HMAPDLHRALAGVMGAG"
BASE COUNT          395 a          590 c          686 g          335 t
ORIGIN      
        1 gtcgtccgcc gcgccggcca tttcccgggg cgggtcgaga tcttccatcc gcaccagcca
       61 gatgccactg cgggcgcggg cgtcgaggaa actggccagc gcggtgagca gcgagcccag
      121 gtgaaggggg ccggtgggcg atggggcgaa gcggccggtg tagggtgcgg ggctcatgaa
      181 gggggacgag cgactctgtc tgctggcgta gaccgcattg tagcggcttt cgccgtccgt
      241 tccctcagtt gctcaccctg cacccattac ccctgccagc gcccggtgca ggtccggtgc
      301 catatgtcgc acgaattcgg tggtgaggtg gtgggaatcc cggaacagaa cgatgccgct
      361 gtcactgtcg acaggattgc agatctcgct ggcacagatg cggcggctca ggtcgatgaa
      421 caggacctgc tccaggtcca ttgcggcctg ccggttgagg ttgtattcaa cggaatccag
      481 cgccagcgtg cgggaaaagt cgcagtccag ttcgagccag ggcgtttttg cctgccgtgc
      541 caggcaggtt ggcacgtcga aaccgggccg cgggctgtcg cgcatgatgg ccaccgcaat
      601 gccggcatcg ttgaaacgct gcattgtttc gcgcatcgcg tcctgccagt gacgttcgct
      661 gatggtggat tgcacggcat cgggcaggga aacataacgg tagtaaccca tcaccaccac
      721 cttggggcgc aacgcgatga ttttttcggt cacctggtcc cgccaccggc tgcactcgtc
      781 atagggccgg cccagttgcg ggttcacggg ttttagccag gccacagggc aggcggattt
      841 tgtcagtgac accagccgcc agtgttgttg acgcgccagt ttttccagcg cgggaaacca
      901 ctgcgcagca tgggaatcgc caaacagcgc gatggtggtg gtggcctccg gatcaccaaa
      961 aaagcagtcg ccggtttcca cggccagcag gctcaggtgg caattggatt cgtaaatggt
     1021 tttcggcaca tcgtccattg cctgggtgaa gcggatctgg gtgggcgacg cggcttccag
     1081 ggtgccggca tgacgccacc aggccgccgt gccggcgctc gccagcgtca ggcacagggc
     1141 gagcgacagt gcatatccgt gctggcgcgc cagacgcgga tggaagcgca ccggattttc
     1201 cacccagcga taggacagat ccgccagcag cagcgacagc accaggcaga gcatggagat
     1261 gcccagatgt ttttcaggca acaacatccc cgccagaatc agtactggcc aatgccacag
     1321 gtaccaggaa taggagcgac cgcctaacca ttgcagcagc ggatgatgca gcaggcgggt
     1381 gcgtaaatcg gtctggccag gggcatggct gatcaggagc aggctggtgc ccagtacggg
     1441 taacagggcc gccacgccgg ggaacgtcgt gtggtgaccg aattgtgttg cggagtaggt
     1501 cagcgccgcc aacccggccc agaaacacgc ggtgcgcagc tgtggtcgcg cggccatcca
     1561 ggcaacaggc acgaagaatg ccagcccgcc gatggcgaat tcccaggcgc gggcgggtga
     1621 actgaaaaag gcccagggtt gcgcaatgtc tgtcagcaag accgacagca gcagcgacag
     1681 ggcggccagt cccgacatgg catacagcag gcgccggcgg ttgggcgtgg cgccttttcg
     1741 tccccgcagc gcgagcagga cgaacagcgg ccagacgaaa tagaactgct cttccaccga
     1801 cagtgaccag gtatgcagca gcggactttt gaccgcatcc gctgcgagat agtcggtggc
     1861 aatgtgggcg aaccagagat tgctggcata ggcggacgtg gctacagcgt ccttggccag
     1921 cgtaatcatg gcctggggtg cgtagatgaa ctgcgcggcc agcagggtga caagcaggac
     1981 gcaggctgcc gccggcagca gtcgac
//
LOCUS       DASUEG010000705         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_229483, whole genome shotgun sequence.
ACCESSION   DASUEG010000705 DASUEG010000000
VERSION     DASUEG010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229483"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX11_13300"
     CDS             <1..294
                     /locus_tag="VFX11_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4939649.1"
                     /translation="DRALRDQVRFEQHNLLQPLEDHGGFDVIFLRNVLIYFDQHTKQR
                     VLEHLVAELRPGGWIITGHCESLFGLKLPLQQLSPSIYRCETWQDPQRARRPA"
     gene            291..1577
                     /locus_tag="VFX11_13305"
     CDS             291..1577
                     /locus_tag="VFX11_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEX4939650.1"
                     /translation="MIYLPAAGNADPEADLHPAILAQGVLVVDDNSVQRANTRACLEA
                     FGIRRIHEAADGASALQCFRSLPVRPAVVVLDLELPGLDGIEVLQRLGDDRHRPAIIV
                     ASAADEVLISAVATMVEALGVPLLGAFRKPVPTAALRAALAAFGHQNSSVLHAAAPVT
                     EIDPAELQQAIRSGQIRPFYQPKISLQQGSLEGLEALARWTDRAGHSIPPSRFIALAE
                     QHGLIGELTLALLGQVLQDMAQWQAQGLQPAVAINLSATSLIDRNLASDILRQVQAAA
                     ISPRRVSFEITETALVADLASALATMSRLRLKGFGFSIDDYGTGFSSMQQLSRFPFTE
                     LKIDRTFVHGAHQRDQLRTILQSAIDMGRRLGITTVAEGVETEAELHLLKAMGCRQAQ
                     GYLIARPMPAAQVSDWIHHRASAVAQLCLQPSTMPT"
     gene            1547..>3589
                     /locus_tag="VFX11_13310"
     CDS             1547..>3589
                     /locus_tag="VFX11_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4939651.1"
                     /translation="MPAAVHDADLANSPGQRSNPVLLLASVAILLLALISFASLRIIH
                     SSHQRITSGYLQTNLQSVLGLIRYWDSQNRGVVRVLVDSSTGRDLIFRVLDEDGARRE
                     TAQALRGWLFPLLLPMGFEGYQVLNLDRRIIAASSANYVGETWLPAEIREALDRALVE
                     GTSISRPVAAHLPMEGPRGMVPPGALMQNLCVVVERAFIKRGYFCLRLNPQRSFFPIF
                     QSGRTGESGEAYAIDRSGRIISPSRFDDVHNILSYSSYDQYSDTPLLARVPAETGQSS
                     GELTAMAQALLAESSASLQAPYSDYRDERVVGSGQWLPELDIGVIVEQDVDEAFAPYV
                     LSSNIIIGLALSAIGLIVVLTASSLMHRRALAARESRFRSLVANIPTSVYLQARDGRF
                     TVVNTAFCELVRIHPDDLLGRLVTELPIPAWLRPVFQTSGDTPGQPDDSIIELQSGDS
                     QRQFFRVVRFPVQRDRGAEYQAVGTIIINVTDRMLAGERLASANQNLERQVVERTTEL
                     MRAKEAAVAASRAKAEFLANMSHEIRTPLNAIIGLSHVALTESLSDRVRNYLEKVRAS
                     GEHLLRVINDILDFSRIEAGKLNVDQVEFALEQVLDKVVDLIWDRADARGLELRVRIE
                     PGLPPVLVGDPLRLGQILINFTANAVKFTDRGHVEIRDSQLQASQQHPNQKDAAPNR"
BASE COUNT          628 a         1226 c         1149 g          586 t
ORIGIN      
        1 gaccgcgcgc tgcgcgacca ggtgcggttc gaacagcaca acctgttgca gccgctggag
       61 gatcacggcg gattcgacgt gattttcctg cgcaatgtac tgatctattt cgaccagcac
      121 accaagcagc gggtactgga acatctggtg gcggaactgc gccccggcgg ctggatcatc
      181 accggccact gcgaatccct gttcggtctg aagctgccgc tgcagcagct gtcgccgtcg
      241 atttaccggt gcgaaacctg gcaggacccc caacgtgcgc ggaggcccgc gtgatttacc
      301 tgcccgccgc cgggaacgcc gatcccgaag ccgacctgca cccggccatc ctggcccagg
      361 gcgtactggt ggtggatgac aacagcgtgc agcgcgccaa cacccgcgcc tgcctggaag
      421 ccttcggcat ccggcggata catgaagcgg ccgacggcgc cagtgccctg caatgctttc
      481 gcagcctgcc ggtgcggccg gcggtggtgg tgctggatct ggaactgccg gggctcgacg
      541 gcatcgaggt actgcagcgg ctgggggacg atcgtcaccg ccccgccatc attgtggcca
      601 gcgcggccga cgaggtgctg atcagcgccg tggccaccat ggtcgaggcc ctgggcgtgc
      661 cattgttggg tgcgtttcgt aaaccggtgc ccacggcggc cctgcgcgct gcgctggcgg
      721 cgttcggcca tcagaacagc agcgtgctgc atgcagcggc cccggtgacc gaaattgatc
      781 cggccgaact gcagcaggcc atccggtccg ggcagatccg gccgttctac cagcccaaga
      841 tcagtttgca acaaggcagc ctggaggggc tggaggcact ggcgcgctgg accgaccgcg
      901 ccggccacag catcccgcct tcgcgcttca tcgcgctggc ggaacagcat ggcctgatcg
      961 gcgaactcac cctggccctg ctggggcagg tgctgcagga catggcgcag tggcaggccc
     1021 agggcctgca gccggcggtg gcgatcaacc tgtccgccac gtccctgatc gaccgcaacc
     1081 tggccagcga cattctgcga caggtgcagg cggcagccat cagtccccgc cgcgtcagct
     1141 ttgaaatcac cgaaaccgcc ctggtggcgg atctggcttc ggccctggcc accatgagtc
     1201 gtctacgctt aaagggattc ggcttctcca tcgacgacta cggcaccggg ttttcatcca
     1261 tgcagcaatt gtcacggttc ccgttcacgg aactgaaaat cgaccgcacc ttcgtgcatg
     1321 gtgcacacca gcgcgaccag ttgcgcacca tcctgcaatc ggccatcgac atgggccggc
     1381 ggctgggcat caccacggtg gcggaaggcg ttgaaaccga agcggaactg cacctgctca
     1441 aggccatggg ctgccggcag gcgcagggtt acctgatcgc ccggccgatg ccggcggcgc
     1501 aggtatccga ctggattcac caccgcgcca gcgccgttgc ccagctatgc ctgcagccgt
     1561 ccacgatgcc gacttagcca atagccccgg ccagcgcagc aatccagtgc tgctgctggc
     1621 cagtgtggcc atcctgctgc tggcactgat cagcttcgcc tccctgcgca tcatccattc
     1681 cagccaccag cgcatcacgt ccggctatct gcaaaccaac ctgcagtcgg tgctgggcct
     1741 gatccgctac tgggacagcc agaaccgggg cgtggtgcgc gtgctggtgg attcgtccac
     1801 ggggcgcgat ctgatctttc gggtgctgga cgaggacggc gcccgccgcg aaaccgcgca
     1861 ggcattgcgt ggctggctgt tcccgctgct gctgccgatg ggattcgaag gctaccaggt
     1921 gctgaacctg gaccgccgca tcatcgctgc cagcagcgcc aactatgtgg gcgaaacgtg
     1981 gctgccggcg gaaatccgcg aagcgctgga ccgggccctg gtggaaggca ccagcatttc
     2041 ccgcccggtg gccgcccacc tgccgatgga aggtccccgg ggcatggtac cgccaggcgc
     2101 cctgatgcag aacctgtgtg tggtggtgga gcgggccttc atcaagcgcg gctatttctg
     2161 cctgcgcctc aatccccagc gcagcttctt tccgatcttt cagagcggcc gcaccggcga
     2221 atccggcgaa gcctatgcca tcgaccgcag cggccgcatc atctccccga gccggttcga
     2281 cgacgtccac aacatcctgt cctactccag ctacgaccag tacagcgaca cgcccctgct
     2341 ggcgcgggtg ccggcggaaa ccgggcagag tagcggcgaa ctgacggcga tggcgcaggc
     2401 cctgctggca gaatccagcg ccagcctgca ggcgccgtac agcgattacc gggacgagcg
     2461 cgtggtggga agcggccagt ggctgccgga actggacatc ggcgtgatcg tggaacagga
     2521 cgtcgacgag gccttcgcgc cctatgtgct gtccagcaac atcatcattg gtctggccct
     2581 cagtgccatc ggcctcatcg ttgtgctcac ggccagctcg ctgatgcacc ggcgtgccct
     2641 ggccgcgcgg gaaagccggt tccgctcgct ggtggccaat attcccacct cggtttacct
     2701 gcaggcccgt gacggccgct tcaccgtggt caacaccgcc ttctgcgagc tggtgcgcat
     2761 ccatccggac gacctgctgg gccggctggt gacagagctg cccattcctg cctggctgcg
     2821 ccccgtcttc caaaccagcg gcgacacgcc ggggcagccc gacgacagca tcatcgaact
     2881 gcagtccggc gacagccagc gccagttctt ccgcgtcgtg cgcttcccgg tgcagcggga
     2941 ccgcggggcc gaataccagg ccgtgggcac catcatcatc aacgttaccg accgcatgct
     3001 ggcgggggag cggctggcca gcgccaacca gaacctggaa cggcaggtgg ttgaacgcac
     3061 cacggaactg atgcgggcca aggaagccgc cgttgccgct tcgcgcgcca aggccgaatt
     3121 cctcgccaac atgagtcacg aaatccgcac cccgctcaac gcgatcatcg gcctgtccca
     3181 cgtggcgctg accgagagcc tcagcgaccg cgtacgcaat tacctcgaga aggtgcgggc
     3241 ctccggcgaa cacctgctgc gggtgatcaa tgacatcctc gacttctccc gcatcgaggc
     3301 cggcaagctg aatgtggacc aggtggagtt cgcgctggag caggtgctgg acaaggtggt
     3361 ggacctgatc tgggaccgcg ccgacgccag gggcctggaa ctgcgggtgc ggatcgaacc
     3421 gggcctgccg ccggtgctgg tgggcgatcc gctgcggctg gggcagatcc tgatcaactt
     3481 caccgccaac gctgtcaagt tcaccgaccg cggacatgtg gaaatccgcg acagtcagct
     3541 gcaggccagc cagcaacacc ccaatcaaaa ggacgcagcc cccaaccgc
//
LOCUS       DASUEG010000706         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_790041, whole genome shotgun sequence.
ACCESSION   DASUEG010000706 DASUEG010000000
VERSION     DASUEG010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790041"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX11_13315"
     CDS             <1..541
                     /locus_tag="VFX11_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4939652.1"
                     /translation="TEISALFLDRVVNLLEEGVDVGIRIGELADSSYKALRVGQVRRV
                     ICASPAYLQHNGVPQQPDDLAQHPLIVASNLNPHVEWRLLRNGEAMTVRIKPRLTVTS
                     NDAAIEAAVRGLGITRLISYQVAPQLAAGELKIVLSDFEPAPVPVHVVHREGRYASAK
                     VRAFIDLMVERLRADPSLN"
     gene            complement(492..1808)
                     /locus_tag="VFX11_13320"
     CDS             complement(492..1808)
                     /locus_tag="VFX11_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX4939653.1"
                     /translation="MKNHAPENSAPENGTPEFRVAIIGGGPAGLMAAEQIARAGVAVD
                     LFDAMPSVGRKLLRAGIGGLNLTHAEASDTLVTRYGKRQTEIAALLARFGRDELLQWV
                     HELGIETFTGSSQRIFPVGKKAAPLLRLWLHRLRGLGVKFHARHRWVGWDADGELIFQ
                     TQNGQLQHRADATVLALGGASWPQLGSDGAWVSWLQQKQIRIAPLQASNCGFVVPWSD
                     HFREKFARQPVKAVTATVVDAQGESHTRNGELMVTDTGLEGGLLYALSAPARDALTQN
                     GSVTLQLDLCPDRTLPQLEQRLAQPQGSRSLAKHLKDKAGIDGVKAGLLREFASADVL
                     HDPQQLAQALKSLPVPLLATQPIANAISSAGGVGFDELSPQLMLKKLPGVFCAGEMLD
                     WEAPTGGYLLTACFASGFVAGIGVLEWLASVSSATDRPATAPPSDR"
     gene            1940..>2556
                     /locus_tag="VFX11_13325"
     CDS             1940..>2556
                     /locus_tag="VFX11_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX4939654.1"
                     /translation="MAGSSLLALLDDIATILDDVAVMSKVAAKKTAGVLGDDLALNAQ
                     QVAGVKADRELPVVWAVCIGSFINKLILVPAALAISVFMPWAITPLLMLGGAFLCFEG
                     VEKLFHKFLHSKVEDEAHHAQQIEALSNPAVDMVAFEKEKVKGAIRTDFILSAEIIVI
                     ALGTVADATLTKQVAVLSTIAILMTIGVYGLVAAIVKLDDGGLYLL"
BASE COUNT          455 a          768 c          807 g          526 t
ORIGIN      
        1 cacggaaatc tcggccctgt ttctggaccg ggtggtgaat ctgctggagg agggtgtgga
       61 tgtcggcatc cgcatcggcg agctggcgga ttccagttac aaggccctgc gggtggggca
      121 ggtgcggcgg gtaatttgtg cgtcgcctgc gtatctgcag cacaacggcg ttccgcagca
      181 gccagatgat ctggcgcagc atcccctgat cgtggccagc aatctgaatc cgcatgtgga
      241 atggcgactg ttgcgcaacg gcgaggccat gacggtgcgg atcaagccgc gtttgaccgt
      301 caccagcaat gacgctgcca tcgaggcggc cgtgcgcggg ctgggcatta cccggctgat
      361 ttcctatcag gtagcgccgc agctggcagc aggcgaactg aagattgtgc tcagtgattt
      421 tgaaccggcg ccggtgccgg tgcatgtcgt tcaccgcgag ggccgttatg cgtcggccaa
      481 ggttcgcgcc tttatcgatc tgatggtgga gcggttgcgg gccgatccgt cgctgaactg
      541 acggacgcca gccattccag cacgccaatg cccgccacaa atccgctggc aaaacaggct
      601 gtcagcagat agccgcctgt gggcgcttcc caatccagca tttcccctgc gcagaacacg
      661 ccgggcagtt tttttagcat cagttgcggc gagagttcat cgaagccgac gccaccggcg
      721 ctgctgatgg cgttagcgat gggctgtgtc gccagcagtg gcaccggcag ggatttcagt
      781 gcctgcgcca gttgttgcgg atcatgcagt acatcagcgc tggcaaattc ccgcagcagg
      841 ccggctttca cgccgtcgat gcccgccttg tctttcagat gctttgccag tgagcgcgaa
      901 ccttgcggtt gcgccagccg ttgttcgagt tgtggcagtg tgcgatccgg gcacagatcc
      961 agctgcagcg tcacgctgcc attctgtgtc agcgcatccc gtgccggcgc cgacagcgca
     1021 tacagcagac cgccttccag cccggtgtcg gtcaccatca gttcgccgtt gcgggtgtgt
     1081 gactcgcctt gtgcatcaac aaccgttgcg gtcaccgcct ttaccggctg gcgtgcaaat
     1141 ttctcgcgga aatgatcgct ccagggcacc acgaatccgc agttgctggc ttgcagcggc
     1201 gctatccgga tttgtttctg ctgcagccag gatacccagg caccatcgga ccccagttgc
     1261 ggccagctgg caccgcccag tgccagcaca gtggcatcgg cgcggtgctg cagctgaccg
     1321 ttttgtgttt ggaaaatcag ttcgccgtcc gcgtcccagc ccacccagcg gtgacgcgca
     1381 tgaaacttca cgcccagtcc gcgcagccgg tgcaaccaca gccgcaacag cggtgccgcc
     1441 ttcttgccca ccggaaaaat ccgctgcgac gagccggtga aggtttcgat ccccagttca
     1501 tgcacccact gcagcagttc gtcgcggcca aaccgtgcca gcaacgcggc gatttcagtc
     1561 tggcgcttgc cgtaacgggt taccagggtg tcactggcct cggcgtgggt caggttcagg
     1621 ccgccgatgc ccgcccgcag cagcttgcgt cccaccgacg gcatggcgtc gaacaggtcc
     1681 actgccacgc cggcacgggc gatctgttcc gccgccatca atccggcggg gccgccaccg
     1741 ataatggcaa cacgaaattc aggggtacca ttttctgggg cactgttttc aggagcgtgg
     1801 tttttcatgg gtgcggtttg cttcgaatgt ggctggcctg ggtgaacccg tagggttaac
     1861 ggatcggggg tggctgatga tacactttcc acccattcga tttcgcccgt attccattgc
     1921 atattggagc cctgagacca tggcaggaag cagtctgtta gccctgttgg atgatatcgc
     1981 caccatcctc gacgacgtgg ccgtgatgag caaggtggcc gccaagaaaa cggcgggcgt
     2041 gctcggcgac gatctggcgc tgaatgccca gcaggtggcc ggcgtgaagg ccgatcgtga
     2101 actgccggtg gtgtgggccg tgtgcattgg ctccttcatc aacaagctga ttctggtgcc
     2161 ggcggcgctg gccatcagcg tgttcatgcc ctgggccatc acgccgctgt tgatgctggg
     2221 cggcgctttt ctctgtttcg aaggcgtgga aaagctgttt cacaaattcc tgcacagcaa
     2281 ggtcgaagac gaagcccatc acgcacagca gatcgaagcg ctcagcaatc ctgccgtcga
     2341 tatggtggcg ttcgaaaagg aaaaagtgaa aggcgccatc cgcaccgatt tcattctgtc
     2401 ggcggagatc atcgtgattg cgctgggcac cgtggcggac gcgacgctga ccaagcaggt
     2461 ggccgtgctg tccaccatcg ccatcctgat gacgatcggt gtgtacgggc tggtggcggc
     2521 cattgtgaag ctggatgacg gtggactgta cctgct
//
LOCUS       DASUEG010000707         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_494518, whole genome shotgun sequence.
ACCESSION   DASUEG010000707 DASUEG010000000
VERSION     DASUEG010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494518"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1735)
                     /gene="amt"
                     /locus_tag="VFX11_13330"
     CDS             complement(284..1735)
                     /gene="amt"
                     /locus_tag="VFX11_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4939655.1"
                     /translation="MKRLLALLALCGVVGFATPVWAEEVAAAEGVVAEAVVAEAAEAA
                     PAPSLSKADNAWIIVAAAFVILMSVPGLALFYGGMVRSKNMLSVLMQVLVVFSLITVL
                     WVIYGYSVAFTEGNAFFGSLSKLFLKGVTPDSLAATWNKGVYISELIYVVFQGAFAAI
                     TCCLILGAFAERIKFSAVLIFSVIWFTFAYLPMAHMVWYWAGPDAYTDADAAAKAGAT
                     AGYLFQMGALDFAGGTVVHINAGIAGLVGAFLVGKRTGFGKESMAPHSLSMTMIGTAL
                     LWFGWFGFNAGSALEANGIAALAFINTWLATAAAALAWVMAEWLIRGKPSMLGAASGA
                     VAGLVAITPACGFVGVGGALVIGLVAGLVCLWAVHNLKRLLGVDDSLDVFGVHGVGGI
                     IGALLTGVFASPDLGGTGVWDYVANGVAEGYSIATQVKIQAISVLTTVVWSTVVAFIA
                     FKLVDLTIGLRVSNDEEREGLDLGSHGEKAYIL"
     gene            complement(1859..2197)
                     /gene="glnK"
                     /locus_tag="VFX11_13335"
     CDS             complement(1859..2197)
                     /gene="glnK"
                     /locus_tag="VFX11_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4939656.1"
                     /translation="MKLVTAIIKPFKLDDVREALSEIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVATSDDLLDQVIESITKAANTGKIGDGKIFVTALEQAIRI
                     RTGETGTDAI"
     gene            2515..2787
                     /locus_tag="VFX11_13340"
     CDS             2515..2787
                     /locus_tag="VFX11_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX4939657.1"
                     /translation="MNRQDTLVNRILELLSERLPEGVTELGQDLRHNLAALVKESLGK
                     MDLVTREEFDVQARVLARTRQRLEQLEQQLQQLESSLQQANQGHQG"
     gene            2843..3238
                     /locus_tag="VFX11_13345"
     CDS             2843..3238
                     /locus_tag="VFX11_13345"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEX4939658.1"
                     /translation="MTNRRFSRFLAPFLAVLTLVTSLQVTVAQAAVVSTGALIQSESA
                     RYDRTRLSDLMQREQAAQALQTLGVDPALVQARIQNMTAEELQAFNDQVNDMEAGGSA
                     LGVIVLVLLLLILLDLLGVTDVFPGIHPA"
     gene            3308..>3705
                     /locus_tag="VFX11_13350"
     CDS             3308..>3705
                     /locus_tag="VFX11_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX4939659.1"
                     /translation="MLLIASGCSLHPGRTDLTPARLPYLPVRHELTSTPFILQDDYQC
                     GPASLAMILGYYGRPATPASLVPWVFTPDAQGSFPAEMDAVTRQQGFLPYPVDRLEDV
                     LAEVAAGHPVLVLQNLATDWYTRWHFAVVVG"
BASE COUNT          789 a         1172 c         1047 g          697 t
ORIGIN      
        1 ttgtccggga aaacccaggc tccaggctcc aagctccagg ctggacagca ttttgattga
       61 tctgccgcgt tgccagcttg cctgcctatc cagaatgctt catccactcc accttgccca
      121 gctaccgccc ccgcgaacgg atggccgcgc tttggtaggg cgaccgcacc ctacatcttc
      181 cctgcgcttt cccgccacca acaaaaacgg gatgccaatg gcatcccgtt tcgtgtgggc
      241 ccgtcagaag cccgggtccg atgtgtccag caatctctgg agattacagg atataggctt
      301 tctcgccgtg agaaccgagg tccagacctt cgcgctcttc gtcgttgctg acgcgcaggc
      361 cgatcgtcag atccaccagc ttgaaggcga taaaggcaac cacagtggac cacaccacgg
      421 ttgtgaggac gctgatagcc tggatcttca cctgggttgc gatcgagtaa ccttcagcaa
      481 cgccattggc aacgtaatcc cacacgccag tgccgcccag atcaggagat gcgaacacac
      541 cggtcagcag cgcgccgatg atgccaccca cgccgtgtac gccgaacaca tccagggaat
      601 cgtccacgcc cagcaggcgc ttcaggttgt gcaccgccca cagacagacc agacccgcca
      661 ccagaccaat caccagtgca ccgcccactc cgacgaaacc gcaggccgga gtgatggcaa
      721 ccagtccggc taccgcaccg gaagcagcgc ccagcatgga gggcttgcca cgaatcagcc
      781 actcagccat tacccacgcc agggcagcgg ccgccgttgc cagccaggtg ttgatgaatg
      841 ccagcgcagc gatgccattg gcttccagtg cagaaccggc gttgaaacca aaccagccga
      901 accacagcag cgcggtaccg atcatggtca tgctcaggct gtgcggtgcc atggattcct
      961 tgccgaaacc agtccgcttg cccaccagga atgcgcccac cagaccggcg ataccggcgt
     1021 tgatgtgcac cacggtgccg ccggcaaaat ccagcgcgcc catctggaac aggtaaccgg
     1081 cagtggcacc ggctttggca gccgcatcag cgtcggtata ggcatcggga cccgcccagt
     1141 accacaccat gtgggccatc ggcagatagg cgaatgtgaa ccagattacg ctgaaaatca
     1201 gaacagcgga gaacttgatg cgctctgcga aggcgcccag gataaggcag caggtgatgg
     1261 cagcgaacgc gccctggaac accacataga tcagctcaga aatgtacacg cccttgttcc
     1321 aggtggccgc cagcgaatcc ggtgtgacac ccttcaggaa cagcttggac agcgagccga
     1381 agaaggcgtt gccctccgtg aacgcgacac tgtaaccgta gatgacccac agcacggtga
     1441 tcagcgagaa caccaccagt acctgcatca gcaccgacag catgttcttg gagcggacca
     1501 tgccgccgta aaacagtgcc agtcccggca cggacatcag gatcacgaat gccgccgcca
     1561 caataatcca ggcgttgtcg gccttgctca gggacggcgc cggcgcggct tcggcagctt
     1621 cggcaacgac ggcctctgct actacgccct ccgcagcggc gacttcctcc gcccagacgg
     1681 gtgtggcgaa ccccaccacg ccacacaggg ccaataatgc taatagtctc ttcatggttg
     1741 actcctctat ttccaatcgc aagtaacaaa aaagggctga acaattccag ggcgcgcggc
     1801 aatcacgcga cacacatccg agcaggcatg gattcggtag tgctggtaag gtaatgcgtt
     1861 aaatggcgtc ggtgccggtt tctccggtgc gaatccggat cgcctgttcc agcgcggtca
     1921 cgaagatctt gccgtcaccg atcttgccgg tattggcagc cttggtgatg gattcgatga
     1981 cctgatccag cagatcgtcg gaggtggcca cttcgatttt cactttcggc aggaagtcca
     2041 ccacatactc ggcaccccga tagagttcgg tgtgaccttt ctgacggcca aaacccttca
     2101 cttcagtgac agtgatcccc tgcactccaa tttcggaaag tgcttcacgc acatcgtcca
     2161 acttgaacgg ttttatgatg gcggtaacca gtttcataga acttctcctg ttttatatat
     2221 gctcgaaagc aaaattgtct ggacggtccc gttatttgca attcagtgca aaatactcaa
     2281 ccagttgccc aatcgcggaa catgtccagc agaaagcaca atccatgcct atttggattt
     2341 ttggcccttt ttcatgcggt taaccggagg aatcaatgaa tcggaccgca tgagcgcccc
     2401 attatatcgc accaaaaaaa ggcacgcccg atttcgaggc agccacaaac tggtgcacac
     2461 aagggtgcgc gaggatcatg gtacactgcc gcactcgcga atacaggagc caacatgaat
     2521 cgtcaggaca cgctggtgaa ccgcatcctc gaactgctgt cagagcgact gccggagggc
     2581 gttacggaac tgggccagga tctgcgccac aatctggcgg cgctggtaaa ggaatccctg
     2641 ggcaagatgg atctggtgac acgggaagag ttcgacgtgc aggcccgcgt gctggcacgg
     2701 acccggcagc ggctcgagca gctggaacag cagctgcaac aactggagtc atcactgcag
     2761 caagcaaatc aggggcatca gggctagaat cacaacagtc cctcccccat ctttctcttc
     2821 cggacactga caggagcaat ccatgactaa ccgtcgtttt tcccgctttt tggccccgtt
     2881 tctggctgtg ctgacgctgg ttaccagcct gcaggtcact gtcgcccagg cggccgtggt
     2941 ctccaccggc gcgctgatcc agagcgaaag cgcgcgctat gaccgcaccc gtctgagcga
     3001 tctgatgcag cgcgaacagg ctgcccaggc cctgcagacg ctgggtgtcg atccggctct
     3061 ggtgcaggca cggatccaga acatgaccgc cgaggaactg caggctttca atgaccaggt
     3121 gaacgatatg gaagcgggcg gcagcgccct gggcgtgatc gtgctggtcc tgctgctgct
     3181 gatcctgctg gatctgctgg gcgtgactga cgtgttcccc ggcatccacc ccgcctgatt
     3241 ccggcgggag ctccgcattt cctctgctcc ataccagtgc agccgccggt gggtggctgt
     3301 gctggggatg ctcctgattg cctcgggatg cagcctgcac ccgggccgca ccgatctgac
     3361 gcctgcccgg ctgccgtatc tgccggtccg gcatgaactc acctccaccc ccttcattct
     3421 gcaggacgac taccagtgtg gtccggcgtc gctggccatg atcctgggct attacggccg
     3481 tcctgccacg ccggcgtcgc tggtgccctg ggtgttcacg ccggatgccc aaggcagttt
     3541 tcccgctgaa atggacgctg tgacccggca gcagggtttc ctgccctatc cggtggaccg
     3601 gctggaggat gtgctggccg aagtggcggc cggccatccg gtgctggtat tgcagaacct
     3661 ggcgacggac tggtacaccc gctggcactt tgctgtggtg gtggg
//
LOCUS       DASUEG010000708         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1842191, whole genome shotgun sequence.
ACCESSION   DASUEG010000708 DASUEG010000000
VERSION     DASUEG010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842191"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFX11_13355"
     CDS             complement(<1..392)
                     /locus_tag="VFX11_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2236 domain-containing protein"
                     /protein_id="HEX4939660.1"
                     /translation="MPTPLKTLQAQVLEQKTRIPSLYGNVDFSIVPERFAATLNDKSL
                     LPPKYARKHRARILADKENVQRALAYTMLGDLVADAYAALMPKYGFKRLIDMLSEACA
                     HGLQAVPNAPQELVDFIQSMERIPDWLD"
     gene            complement(549..1991)
                     /locus_tag="VFX11_13360"
     CDS             complement(549..1991)
                     /locus_tag="VFX11_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939661.1"
                     /translation="MQLERTLTSPLSVATVFQYLAQFHRVVEWDASVTRARKLSAGPI
                     GVGTQFEVDVHFLGQTTRLVYTLLEFEPCTRLVLRGTADLYQVVDTIHFASTPSGGTA
                     LRYQLSVEYTARLKRIAGIFAPLVRRNVDDAITGLTRTLNDSPQKVEPYPLWRDKFIL
                     PGALEFTRWGFRNSKKHWKGLSQDLRGQHILITGASSGLGRAATLALAEMGAHVIAVV
                     RDHAKGAQLQQDVQDYCGATLQLEYADLASLTQTVALAQRLRNRDQPLQVLINNAGAL
                     FNTRQLTAEGLEKSFALLLASPFALTELLLPLLRQNDSARVINVVSGGMYTQAVRPQD
                     LNYEQETYDGPKAYARAKRGLVDMTRWWAQQPENRHVIFHAMHPGWADTQAVADSLPG
                     FYRTMQRWLRTPEQGADTIVWLAAAREPLHRNGEFWLDRRPHTTAIFPGTESSAETRL
                     ELQKHLHRLLQTFTPIDTAQGQADATTARE"
     gene            complement(2140..>2857)
                     /gene="rpoH"
                     /locus_tag="VFX11_13365"
     CDS             complement(2140..>2857)
                     /gene="rpoH"
                     /locus_tag="VFX11_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX4939662.1"
                     /translation="VDAARHLVLSHLRFVVHIAKSYSGYGLPQADLIQEGNVGLMKAV
                     KRFDPTVGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRSQKKRLGW
                     MTNDEIEAIAQDLGVDAADVREMEGRLGAFDTSFDAPVDADDETASQAPVFYIEDENA
                     DPALQLEQDDWDNTHHARLHLALEKLDDRSRDILEQRWFADDKSTLHDLAAKYDVSAE
                     RIRQLEKNAMKKLKDAILQA"
BASE COUNT          555 a          849 c          852 g          601 t
ORIGIN      
        1 atgtcgagcc aatccggtat gcgctccatc gactggatga aatccaccag ctcctgtggc
       61 gcattcggca ccgcctgcag accgtgcgca caggcctcgg acaacatgtc gatcaggcgc
      121 ttgaaaccgt acttgggcat cagcgcagca taggcatccg ccaccagatc acccagcatg
      181 gtgtaggcca gcgcccgctg cacgttctcc ttgtccgcca gaatccgggc ccggtgtttg
      241 cgcgcatatt tcggcggcaa caggctcttg tcattcaggg tggcggcgaa ccgctcgggc
      301 acgatgctga aatccacatt cccgtacaac gacggaatcc gggtcttttg ctccagcacc
      361 tgggcctgga gggttttcag gggggtcggc atgactggct acgctcatca aacagtgatt
      421 gttgttatag aacccacggg ttctgtgatt aagggtagtg ccagcagggc agaacaggca
      481 atgtttttgt gcgccaactt gacagttgtt ttattgactt actgatttag ttggccagac
      541 ctgggcttct attcacgcgc tgtcgtagcg tcagcctgac cttgcgcggt gtcgatgggc
      601 gtgaaggttt gaaggagccg atgcagatgc ttctgcagtt ccaggcgcgt ttcggccgaa
      661 ctttcggtac cgggaaaaat ggcggtggtg tgggggcgac ggtcgagcca gaactcgccg
      721 ttgcggtgca gcggttcccg cgccgccgcc agccagacga tggtgtcggc gccctgttcc
      781 ggcgtacgca gccagcgctg catggtgcga tagaagccgg gcagtgaatc cgccaccgcc
      841 tgggtgtcgg cccagcccgg gtgcatggcg tggaatatca cgtgccggtt ctcaggctgc
      901 tgcgcccacc agcgggtcat gtccaccagt ccgcgcttgg cgcgggcata ggccttgggg
      961 ccgtcgtagg tttcctgttc gtaattcaga tcctgcggac gcacggcctg cgtatacatg
     1021 ccgccggaca ccacgttgat gacacgggca ctgtcgttct ggcgcagcag cggcaacaac
     1081 agttcggtga gcgcgaacgg gctcgccagc agcagcgcga aggatttttc cagcccttcc
     1141 gccgtaagct gacgggtgtt gaacagcgcg cccgcattat tgatcaacac ctgcagcggc
     1201 tgatcacgat tgcgcaggcg ctgtgccagc gccaccgtct gggtcagcga cgccagatcc
     1261 gcatattcca gttgcagggt tgcgccgcaa taatcctgca cgtcctgctg caactgcgcg
     1321 cccttggcgt gatcccgtac cacggcaatc acgtgagcgc ccatttccgc cagtgccagt
     1381 gtcgccgccc ggcccagacc ggacgaggct cccgtgatca gaatatgctg cccgcgcaga
     1441 tcctggctca gacctttcca gtgctttttg ctgttgcgaa aaccccagcg ggtgaattcc
     1501 agcgcaccgg gcagaatgaa cttgtcgcgc cagagcggat agggttccac tttctgcggt
     1561 gaatcattga gtgtgcgggt caggccggta atggcgtcgt ccacgttgcg tcgcaccaac
     1621 ggcgcgaaaa tgccggcgat ccgtttcagc cgcgccgtgt attccacgct cagctgatag
     1681 cgcagcgcgg tgccaccgga cggcgtggat gcaaaatgaa tggtatcgac cacctgataa
     1741 agatcggcgg ttccgcgcaa gaccagacgg gtgcaaggct cgaattccag cagggtataa
     1801 accagccgcg tggtctgccc cagaaaatgc acatccactt caaattgcgt gccgacgcca
     1861 attggacccg cgctgagttt gcgtgcgcgt gtcaccgacg catcccactc caccacgcgg
     1921 tgaaactgtg ccagatactg aaacacggtt gcgacggaca aaggcgaggt aagcgtgcgt
     1981 tccagttgca tgagaactcc gggtgggaaa atgcggcaaa cgttcagatt gtgtaacctg
     2041 gacccacggc cggaatccag ttcgcctgca ccagaaacaa aaacgggatc agtcgacgct
     2101 gactgatccc gtggtattgt ttcaatggca ttccaatgtt tatgcctgca ggatcgcgtc
     2161 cttcagcttt ttcattgcat tcttttccag ctggcgaatc cgctcggcgg acacatcata
     2221 cttcgcggcc agatcgtgca gcgtggactt gtcatcagca aaccagcgct gctccagaat
     2281 atcgcggctg cggtcatcaa gtttttccag cgccaggtgc aggcgggcgt gatgggtgtt
     2341 gtcccagtca tcctgttcca gttgcagcgc cggatcggca ttttcgtctt cgatgtagaa
     2401 caccggggcc tgcgatgcgg tttcatcgtc ggcatccacc ggtgcatcga acgaggtgtc
     2461 gaacgcaccc agccggcctt ccatttcccg cacatcggcg gcgtccacac ccagatcctg
     2521 ggcgatggct tcgatctcgt cgttggtcat ccagccgagg cgttttttct ggctgcgcag
     2581 attgaagaac agtttgcgtt gcgccttggt ggtggcgatc ttcacgatgc gccagttgcg
     2641 caatacatac tcgtgaattt cagccttgat ccagtgcaca gcgaacgaca ccagacgcac
     2701 gcccacggtc gggtcaaaac gtttcaccgc tttcatcagg cccacgttgc cttcctggat
     2761 caggtcggcc tggggcaggc cgtaaccgct gtaggatttg gcgatatgaa cgacaaagcg
     2821 cagatgagaa agcaccagat gacgggctgc atctacg
//
LOCUS       DASUEG010000709         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_595202, whole genome shotgun sequence.
ACCESSION   DASUEG010000709 DASUEG010000000
VERSION     DASUEG010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595202"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX11_13370"
     CDS             <1..211
                     /locus_tag="VFX11_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HEX4939663.1"
                     /translation="HGLALNVDMDLEPFHRINPCGYQGLQMAQVKEFVNQPDLAAISA
                     RLADHLANNLGYTERQISTSPWTPA"
     gene            208..1242
                     /gene="lipA"
                     /locus_tag="VFX11_13375"
     CDS             208..1242
                     /gene="lipA"
                     /locus_tag="VFX11_13375"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX4939664.1"
                     /translation="MNPEVPESGTPCSTHQPPAKKQVLQGEKLRGAEKVARIPVKVIP
                     TTDLPRKPDWIRVRVPANGEVQRVKNLLRQQKMHTVCEEASCPNLPECFGGGTATFMI
                     MGDICTRRCPFCDVAHGRPNALDPDEPKNLAESVAALALKYVVVTSVDRDDLRDGGAG
                     HFADCIRAIRTQSPSTKIEVLVPDFRGRMEIALDLLAADAPDVFNHNMENVPRMYKAI
                     RPGSDYQWSLDLLKRYKARVPHVPTKSGIMVGLGETNEEVFQTLRDLRAHDVDMITIG
                     QYLQPSKQHAPVDRFVTPAEFEEFRIFGEELGFANVASGPLVRSSYHADLQHKGEDVN
                     RIHQASLKPE"
     gene            1383..1649
                     /locus_tag="VFX11_13380"
     CDS             1383..1649
                     /locus_tag="VFX11_13380"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX4939665.1"
                     /translation="MNRLAIAACPLFLSLPAANAAIEATKPERPVIKLDFCLDGKKVE
                     ARANLADREGFLYPALIGRNVLKTGDYMIDPQRSFIHEPGCKKK"
     gene            1759..>2067
                     /locus_tag="VFX11_13385"
     CDS             1759..>2067
                     /locus_tag="VFX11_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX4939666.1"
                     /translation="MEHYLDVLMQWLNQHQDWILFFLALLSFLECLALVGIVVPGVVL
                     LVGVSTAAGSAGMDVWHVLLAGFIGAALGDGVSYLLGYHYHHVIRRLPPFSTHPHWIE
                     K"
BASE COUNT          413 a          677 c          595 g          382 t
ORIGIN      
        1 tcacggtctg gccctcaacg tcgacatgga cctggaaccc ttccaccgta tcaacccctg
       61 cggttatcag ggcttgcaga tggcgcaggt gaaagaattt gtaaaccagc cggatttagc
      121 cgccatcagc gcccgtctgg cggatcatct ggccaacaat ctgggatata ctgagcggca
      181 aatcagcaca tccccctgga cccccgcatg aaccccgaag tacccgaatc cggcaccccc
      241 tgcagcaccc atcagccccc ggccaaaaag caggtcctgc agggcgaaaa gctgcgcggc
      301 gcggaaaaag tcgcgcgcat tccggtgaag gtgattccca ccaccgacct gccgcgcaaa
      361 ccggactgga ttcgggtgcg ggtgccggcc aacggcgaag tgcaacgggt gaagaacctg
      421 ctgcggcagc agaagatgca cacggtgtgc gaagaggcgt cctgccccaa cctgccggag
      481 tgcttcggcg gcggcaccgc caccttcatg atcatgggcg acatctgcac ccgccgctgc
      541 ccgttctgcg acgtggccca cggccgcccc aatgcacttg atccagatga accgaaaaac
      601 ctggcggaat ccgtcgccgc cctggccctg aaatatgtgg tggtcacctc ggtggaccgc
      661 gacgacctgc gcgacggtgg cgctggccat ttcgccgact gcatccgcgc catccgcacc
      721 cagtcgccca gcaccaagat cgaagtgctg gtgccggatt tccgcggccg catggagatc
      781 gcgctggacc tgctggccgc cgacgcgccg gacgtgttca accacaacat ggaaaacgtg
      841 ccacggatgt acaaggcgat ccgcccgggt tccgactacc agtggtcgct ggatctgctg
      901 aagcgctaca aggcccgcgt gccgcacgtg cccaccaagt ccggcatcat ggtcggactg
      961 ggcgagacca acgaggaagt gttccagacc ctgcgcgacc tgcgcgccca cgacgtcgac
     1021 atgatcacca tcggccagta tctgcaaccg tccaagcagc acgcgccggt ggaccgcttc
     1081 gtcaccccgg cagagttcga ggaattccgc attttcggcg aggaactggg ctttgccaac
     1141 gtggccagcg gcccgctggt gcgctcgtcc tatcacgcgg atctgcagca caagggcgaa
     1201 gatgtgaacc gcatacacca ggccagtctg aagcctgaat aagccccccg tcgcatcttt
     1261 cagaattgct tgaggacgtt ttgctattac gccccctaca atcacgccaa ctgccaacac
     1321 gaagcccttc cagcacggac gggtttccgg tttcttcccg cccccaacaa gcggaacgtg
     1381 atatgaacag actcgcgatt gccgcttgcc ccctcttcct ttcactaccg gcggccaacg
     1441 ctgcaattga agccaccaag ccggaacgcc cggtgatcaa gctggacttc tgcctggacg
     1501 gcaagaaggt ggaagcccgt gccaacctgg ctgaccggga aggctttctg tatccggctc
     1561 tgatcggacg caacgtgctc aaaaccggtg actacatgat tgacccgcaa cgttccttca
     1621 tccatgagcc aggctgcaag aaaaagtagc gggcgcgggc gcgacccgtt ccgctcaatg
     1681 cgctaccggc tgctgacaac ggtgtatttc acctttgcgc agtttacaat ccggacactt
     1741 ccgtttttca ggtaacgcat ggaacactac cttgacgtgc tgatgcagtg gctcaaccag
     1801 catcaggact ggattctctt tttccttgcc ctgctgtcgt ttctggaatg cctggcgctg
     1861 gtgggcatcg tcgtgcccgg cgtggtgttg ctggtgggtg tgtcgactgc cgctggcagc
     1921 gcaggcatgg atgtctggca tgtcctgctg gcggggttca tcggcgcggc actgggggat
     1981 ggcgtcagct atctgctggg ctatcactac catcatgtca tccggcgcct gccgccgttc
     2041 agcacgcatc cgcactggat tgaaaag
//
LOCUS       DASUEG010000710         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1643438, whole genome shotgun sequence.
ACCESSION   DASUEG010000710 DASUEG010000000
VERSION     DASUEG010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643438"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VFX11_13390"
     CDS             complement(<1..1315)
                     /locus_tag="VFX11_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939667.1"
                     /translation="MIIQSSNLRFASTSSRREREETSVQGSLTRGAPVQGNPVSISAL
                     YARKENVRLQVAQSSYLYSRSQVSRADQPQNVQVHSREEALQSLVSHAFSRDIRITGN
                     ASLGSSASSGRLSPQGQPLASQVTATLSRSYRYETEQHSALTIEGQLQLADCRTVDFV
                     LHTRMDSSLQFQSGSGQFASAAVRTDPLIINLHGGAAQLTDTAFTFDIDGDGSSESVS
                     FATGGSGFIAFDRNGDGKINDGSELFGSRSGDGFADLAALDDDGNGFLDQNDAAFAQL
                     KFYSRDENGNEILQNLSEVGVAALGVQSADSPALVRGSAGQELGLVRGTGVFVLESGA
                     VGTVQQVDLANRDLKAEKDFAADFDNAQFVPPAEDGAVTDPRLQDVQDAMQRLEKIHG
                     DFMARLDALQHPEENEDSKTLLEMLVDKLEEFVTQKSEKVEKDKKD"
     gene            1498..2562
                     /locus_tag="VFX11_13395"
     CDS             1498..2562
                     /locus_tag="VFX11_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4939668.1"
                     /translation="MKQLLSDFKEGFPLGRVTPLTDRVHVLSLPMQFHPGHINTYLLE
                     DDDGLTLVDTGNVGPDNQRFWQDVLDSPLARKGVKRVLLTHGHPDHCGLAEWLCQTTG
                     AELWLTAPELDAVRRLWRGSPLNYDQVKPFFLQWGLPEEHFPAVQGLLDSFRFGTSDL
                     DVPVIRLIEEGEILTLAGQPWQVKCGFGHTPSNATLWQRETGLLITGDHLLPRISPNI
                     SLWWGSSSDPLGEYLDSIQTFRGMGDLVGLPSHGTTFGDLDTRIDMLTRFHCKRIQRA
                     LEFCRDQPRSAYECVHPVLGKADSGAMIALIAGQAFAILAFLEQAGLVQRVDGDIFRF
                     QTLPDAGERLHRLPVFAQRE"
     gene            2623..>3137
                     /locus_tag="VFX11_13400"
     CDS             2623..>3137
                     /locus_tag="VFX11_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939669.1"
                     /translation="MAFGFKRTATLCLLGAVALQAAGSLQAGALASPSAGPLHARLQS
                     VHGGRDQIPVDRLIIKFKPAAQAVVQRSLQPSAQIRESIPALQRLASRHQLQGRPLRR
                     LPNGSHVVQLDQQRSPADIAAVISQFILDPDIEMVEVDLRMFPAAIPTDPLYVSQWAL
                     SEATGGVRPQAG"
BASE COUNT          522 a          964 c         1003 g          648 t
ORIGIN      
        1 ggtctttctt gtctttttcg accttttcag atttttgggt cacgaattct tccagcttgt
       61 ccaccagcat ttccagcagg gtcttgctgt cttcgttttc ctcaggatgc tgcagggcgt
      121 ccagccgggc catgaaatcg ccgtggattt tctccagccg ctgcatggcg tcctggacgt
      181 cctgcaggcg cgggtcggtg acggcgccat cttcggcagg cggcacaaac tgggcgttgt
      241 cgaaatcggc ggcgaagtct ttttcagctt tcaggtcgcg gttggccagg tccacctgct
      301 gcacagtgcc cacggcgccg gattccagca cgaacacgcc ggtgccacgc accaggccca
      361 gttcctgacc cgcactgccg cgcaccaggg cgggggaatc ggcactctgc acgcccaggg
      421 cggccacgcc cacttcactc aggttctgca ggatttcgtt gccattttcg tcgcgggaat
      481 agaatttcag ttgcgcgaag gcggcgtcgt tctggtcgag gaaaccgttg ccgtcgtcgt
      541 ccagcgccgc cagatcggca aagccgtcgc cgctgcggct accgaacagt tcgctgccgt
      601 cgttgatctt gccgtcgcca ttgcgatcga aggcgatgaa gccgctgcct cctgtggcga
      661 acgacacgga ttcgctgctg ccgtcgccgt cgatatcaaa cgtgaaagcg gtgtcggtca
      721 actgcgcggc gccgccgtgc aggttgatga tcaggggatc cgtgcgcacg gccgcgctgg
      781 cgaactggcc gctgccgctc tggaattgca ggctgctgtc catgcgggtg tgcagcacga
      841 aatccacggt gcggcagtcc gccagttgca gctggccttc gatggtgagg gcggaatgct
      901 gttcggtctc gtaacggtag gaccggctca gggtggcggt cacctggctg gccaggggct
      961 ggccctgcgg gctcaggcgg ccgctgctgg cggaactgcc gaggctggca ttgccggtga
     1021 tgcggatgtc gcgggaaaaa gcgtgggaca ccagcgattg cagggcttcc tcgcgactgt
     1081 gtacctgcac gttctgcggc tggtcggcgc ggctgacctg gctgcggctg tatagatagc
     1141 tggactgcgc cacctgcagg cgcacgtttt ccttgcgggc ataaagggcg ctgatggaga
     1201 ccggattgcc ctggacgggt gcgccgcggg tcaggctgcc ctggacgctg gtttcttccc
     1261 gttcacggcg gctgctggtg ctcgcgaaac gcagattcga cgactggatg atcataaggg
     1321 cccggaatga gagttgaggc tatatccgcc ttatcggctg cactggagat ttcttgagca
     1381 ctgtgtctgc ctgataacgc tatatgccga tcaatccggt caccctgtcc tgccattgcc
     1441 cgcgaccggc cggttcctgt atggtcagtg tttgcccagt cacaaccaag atgcggaatg
     1501 aaacagttac tgtctgattt taaagagggt tttcccctgg ggcgggtcac accgctgacc
     1561 gatcgtgtgc acgtgctcag cctgcccatg caattccatc ccgggcatat caacacctat
     1621 ctgctggaag acgacgacgg cctgaccctg gtggacaccg gcaacgtggg gccggacaac
     1681 cagcggttct ggcaggatgt gctggattcg ccgctggccc gcaagggcgt gaagcgggtg
     1741 ctgctcaccc acggccatcc ggatcactgt gggctggcgg aatggctgtg ccagaccacc
     1801 ggggctgaac tctggctcac cgcgccggaa ctggatgcgg tgcgccggct gtggcgggga
     1861 tcgccgctca attacgatca ggtgaagccg tttttcctgc aatggggtct gccggaggaa
     1921 cattttcccg ccgtgcaggg gctgctggac agcttccgtt tcggtaccag cgatctggat
     1981 gtgcccgtga tccgcctgat tgaagagggc gagattctca ccctggcggg ccagccctgg
     2041 caggtgaaat gcggctttgg ccatacgccc agcaacgcca ccctgtggca gcgtgagacc
     2101 gggctgctga tcaccggcga tcacctgctg ccgcgcattt ccccgaatat cagcctgtgg
     2161 tggggcagca gttccgatcc actcggtgag tatctggatt cgatccagac ctttcgtggc
     2221 atgggcgatc tggtgggcct gccgtcccac ggcaccacct ttggtgacct ggatacgcgc
     2281 atcgacatgc tcacccgctt tcactgcaag cgcatccagc gggccctgga attctgccgc
     2341 gaccagccgc ggtccgccta tgaatgcgtg catccggttc tgggcaaggc cgacagcggt
     2401 gccatgattg cattgattgc cgggcaggct tttgccattc tggcgtttct ggagcaggcg
     2461 ggtctggtgc agcgggtgga cggtgatata ttccgctttc agaccctccc cgacgccggc
     2521 gagcgactgc accggctgcc ggtctttgcg cagcgggaat agacgtccac gctgaggcac
     2581 ccggttcccg acaacaaaaa caagaacgac gaggagcggt ttatggcatt cggattcaag
     2641 cggacagcca cgctgtgcct gctgggcgcg gtggcccttc aggcggcagg ttctcttcag
     2701 gcaggcgcgc tagcaagccc atcggcaggc ccactgcatg caaggctgca gtcagttcac
     2761 ggcggccgcg accagatccc ggtggatcgc ctgatcatca aattcaaacc cgctgcccag
     2821 gccgtcgtgc agcggtcact gcaaccttcc gcccaaatcc gtgaatcaat acccgcattg
     2881 caacggctgg cctcgcgcca ccagttgcag ggccgtccgt tgcggcgcct gcccaacggt
     2941 tcccatgtgg tgcaactgga ccagcagcgg tcgccggccg acattgccgc tgtcatcagc
     3001 cagtttattc tcgatcctga tatagaaatg gtggaagtgg acctgcgcat gtttcccgcc
     3061 gcaattccca ccgatccgct ttatgtgtcg cagtgggcgc tgagcgaggc taccggcggc
     3121 gtgcgtccgc aagcggg
//
LOCUS       DASUEG010000711         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_24771, whole genome shotgun sequence.
ACCESSION   DASUEG010000711 DASUEG010000000
VERSION     DASUEG010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24771"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFX11_13405"
     CDS             <1..477
                     /locus_tag="VFX11_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX4939670.1"
                     /translation="SESIEIPINGLFVMGLTLIIAIGVALMMYRSRWGCQVRAVVQNR
                     TMAGAVGINTEKVDRMTFALGCGIAGIAGSAFTMIGSTGPTSGQLYIVDTFLVVVFGG
                     ASSLLGTIASAFTISQAQSTMEFFMSGSMSKVLTLLVVVGILMLRPQGLFALKIRR"
     gene            510..1649
                     /gene="urtC"
                     /locus_tag="VFX11_13410"
     CDS             510..1649
                     /gene="urtC"
                     /locus_tag="VFX11_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010564284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX4939671.1"
                     /translation="MSYQGFKSYLPQQDLLGFALLAVLLLVIFPLSMDIFRLNLVGKY
                     LTYAFVALGLVLCWGYGGILSLGQGVFFGLGGYCMAMFLKLEASNPEATKIQSTPGIP
                     DFMDWNQLTELPFFWEPFHSLTFTLIAVVLVPSIFAYFIGVAMFKRRVGGVYFAIITQ
                     ALAAILTILIIGQQGYTGGVNGITDLRTLKGWDIRSDEAKYILYFVNVAMLFLCLLTA
                     QYVRKSKLGRLLVAMREREDRVRFSGYDVSNFKIFVFCLGAAFSAIGGAMFTLQVGFM
                     SPSFVGIVPSIEMVIFCAVGGRLSLLGAVYGTLLVNWAKTSFSESFPELWLLAMGGLF
                     IAVVLAFPNGLAGLYQTWVAPRIDKWLASLKTPRPLTTNPLESRS"
     gene            1653..2417
                     /gene="urtD"
                     /locus_tag="VFX11_13415"
     CDS             1653..2417
                     /gene="urtD"
                     /locus_tag="VFX11_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX4939672.1"
                     /translation="MTISNVSIGAKDFVLAVEGLTVSFDGFKAVNDLSFYVDENEIRV
                     IIGPNGAGKTTVLDLICGRTKATTGSIKFRGKELTKMREHEIVHAGVGRKFQNPSIYE
                     DLTVFENLELSYPKGRNVWGALTFKRDREVISRVEEIAEMIFLKDLLGKEAGLLSHGQ
                     KQWLEIGMLLIQNPDLLMLDEPVAGMSVAERKQTAELLHRITKNRSVIVIEHDMQFVE
                     DIADRVTVLHQGKVLSEGSMERVKTDPKVVEVYLGH"
     gene            2428..>2752
                     /locus_tag="VFX11_13420"
     CDS             2428..>2752
                     /locus_tag="VFX11_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4939673.1"
                     /translation="MLSVSQYQVAYGQSEVIHNLNFEIKPKEIVAIMGRNGMGKTTLM
                     KSLMGILPSRKGSVKLDGTDLTAMKSHQRVASGVGFVPQGRMIFSTMTVKENIETGLT
                     TVKDKK"
BASE COUNT          570 a          815 c          778 g          589 t
ORIGIN      
        1 agtgaatcca tcgaaattcc catcaatggc ttattcgtca tgggcctgac gctgatcatc
       61 gccattggcg tggcgctgat gatgtaccgc tcgcgctggg gctgtcaggt gcgtgctgtg
      121 gttcagaacc gcaccatggc cggcgccgtc ggcatcaaca cggaaaaagt ggatcgcatg
      181 acgttcgcgc tgggttgcgg catcgccggc atcgcgggca gcgccttcac catgatcggt
      241 tccaccggcc ccacgtccgg tcagctttat attgtggaca ccttcctggt cgtcgtgttc
      301 ggcggcgcat ccagcctgct cggcaccatc gcatcggcgt tcaccatttc gcaggcacaa
      361 tccaccatgg aatttttcat gagcggttcc atgtcgaagg tgctcacgct gctggtggtg
      421 gtcggcatcc tgatgctgcg accacaaggc ctgttcgcac tgaagattcg tcgctaagca
      481 tcctggcaac gcagtaagga gaaaacacga tgtcatatca aggtttcaaa tcctacctcc
      541 cgcaacagga cctgctcggc ttcgcgctgc tcgcggtcct gctgctggtg atttttccgc
      601 tgtcgatgga cattttccgc ctcaacctgg tgggcaaata tctcacctac gcgtttgttg
      661 cgctgggcct ggtgctgtgc tggggttacg gcggtatcct cagtctgggc cagggtgttt
      721 ttttcggcct cggcggctac tgcatggcga tgttcctgaa actggaagcg tccaatccgg
      781 aagccaccaa gatccagtcc acgccgggca taccggattt catggactgg aatcaactga
      841 cggagctgcc gtttttctgg gaaccgttcc acagcctcac cttcacgctg attgcggtcg
      901 tgctggtgcc atccatcttc gcctatttca tcggcgtggc catgttcaaa cgccgtgtcg
      961 gtggcgtgta cttcgccatc atcacccagg cgcttgctgc gatcctcacc atcctgatca
     1021 tcggtcagca gggctacacc ggcggcgtca acggcatcac cgacctgcgt acgctgaaag
     1081 gctgggacat ccgctccgac gaagcaaaat acattctgta cttcgtcaac gtggccatgc
     1141 tgttcctgtg tctgctcacg gcgcaatacg tgcgcaagag caagctcggc cgcctgctgg
     1201 tggcgatgcg ggaacgggaa gaccgtgtgc gcttctcggg ctacgacgtt tccaatttca
     1261 agatcttcgt gttctgcctc ggcgccgcat tctctgccat cggtggcgcc atgttcaccc
     1321 tgcaggtggg tttcatgtcg ccgtccttcg tcggcatcgt gccgtccatt gaaatggtaa
     1381 ttttctgcgc cgtcggcggt cgcctgtcac tgctgggtgc ggtgtacggc acgctgctgg
     1441 tgaactgggc caaaaccagt ttctcggaaa gttttccgga attgtggctg ctcgccatgg
     1501 gcggcttgtt catcgcggtg gtactggctt tccccaatgg cctggccggt ttgtatcaaa
     1561 cctgggtggc gccgcgcatc gacaaatggc tcgccagcct gaaaacgccg cgaccgctca
     1621 ccaccaaccc gctggaatcc aggagctgaa ccatgaccat ttccaacgta agcatcggcg
     1681 ccaaggattt cgtcctcgca gtggaaggac tcaccgtatc cttcgacgga ttcaaggcgg
     1741 tgaacgatct gtcgttctac gtcgatgaaa atgaaatccg cgtgatcatc gggccgaacg
     1801 gcgccggcaa aaccacggtg ctggatctga tctgcggccg caccaaggcc accaccggtt
     1861 ccatcaagtt ccgcggcaag gaactcacca agatgcgcga gcatgaaatt gtccacgccg
     1921 gcgttggccg caaattccag aatccatcga tctatgagga tctcactgtc tttgaaaacc
     1981 tggagctctc ctaccctaaa ggacgcaatg tgtggggcgc gctcaccttc aagcgggaca
     2041 gggaagtcat ctcccgggta gaggaaatcg cggaaatgat tttcctcaag gatctgctcg
     2101 gcaaggaagc gggcctgctc agccacggcc agaaacagtg gctggaaatc ggcatgctgc
     2161 tgatacaaaa cccggatctg ctgatgctcg acgaaccggt ggccggcatg tcggtcgccg
     2221 aacgcaaaca gaccgccgaa ctgctgcacc gcatcaccaa gaaccgttcc gtgatcgtga
     2281 tcgaacacga catgcagttc gtggaagaca tcgccgaccg cgtaaccgtg ctgcatcagg
     2341 gcaaggtgtt gtcagaaggc tccatggaac gggtcaagac cgatcccaaa gtcgtcgaag
     2401 tgtatctggg ccattgagga gaacgccatg ctttccgtat cacagtacca ggtggcctat
     2461 ggccagagcg aagtcatcca caacctgaat ttcgaaatca agccgaagga aatcgtcgcc
     2521 atcatgggcc gcaacggcat gggcaaaacc acgctgatga aatccctgat gggcatcctg
     2581 cccagccgca agggcagcgt gaaactggac ggcaccgatc tcacggcgat gaaaagccac
     2641 cagcgcgtcg ccagtggtgt cggcttcgtg ccccagggac gcatgatttt ttccaccatg
     2701 accgtgaagg aaaacatcga gaccggactg accacggtga aagacaaaaa ag
//
LOCUS       DASUEG010000712         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2091687, whole genome shotgun sequence.
ACCESSION   DASUEG010000712 DASUEG010000000
VERSION     DASUEG010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091687"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1622)
                     /gene="ahpF"
                     /locus_tag="VFX11_13425"
     CDS             complement(57..1622)
                     /gene="ahpF"
                     /locus_tag="VFX11_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEX4939674.1"
                     /translation="MLDTTLKGQLQAYLERVVYPLEINASVDDSAHSQEMLDLLNDIV
                     GLTDKIKLNVSRDDKERKPSFALYRAGANMGSVRFAGIPMGHEFTSLVLALLQVGGHP
                     PKVEPEVIEQIKSLDGKFNFETFISLTCHNCPDVVQALNLLAVLNPNISHVMIDGALF
                     QDEVNQREIMGVPAVYLNGEPFGQGRMTLEEILGKIDTGAAAREAAKLEQKEAFDVLV
                     VGGGPSGSSAAIYAARKGIRTGVAAERFGGQVMDTMAIENFISVLETEGPKLAGALEA
                     HVKHYNVDIMNNQRATALVPASQSGDGMVHIKFENGASLRSKTVILSTGARWRNMNVP
                     GETEYRTKGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLAGIVEHVTVLEFDSKL
                     RADAILQKKMYSMNNITVILSAQTTEVTGDGHKMDGLIYKDRVSGDVHRVDVAGVFVQ
                     IGLVPNTDWLKGTVELSNRGEIIVDDRGQTSVPGVFAAGDCTTVPYKQIIIAMGEGAK
                     ASLAAFDYLIRNS"
     gene            complement(1753..2316)
                     /gene="ahpC"
                     /locus_tag="VFX11_13430"
     CDS             complement(1753..2316)
                     /gene="ahpC"
                     /locus_tag="VFX11_13430"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEX4939675.1"
                     /translation="MSLINTQVQPFKTQAFHNGKFVEVTEQSLKGKWSVLIFMPAAFT
                     FNCPTEVEDAADNYAEFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     QLTNAFGVHIPEEGLALRGTFIINPEGVIKTLEVHDNAIARDVSETLRKLKAAQYVAS
                     HPGQVCPAKWKEGAKTIAPSLDLVGKI"
     gene            complement(2539..2627)
                     /locus_tag="VFX11_13435"
     tRNA            complement(2539..2627)
                     /locus_tag="VFX11_13435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2591..2593),aa:Leu,seq:taa)
     gene            2773..3363
                     /locus_tag="VFX11_13440"
     CDS             2773..3363
                     /locus_tag="VFX11_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939676.1"
                     /translation="MAISVSEDYIRVYRAWNGKEYQVYYPLKKNGRFLTEEELLETLE
                     EAQAKDVELAQRQRAHFMRKDLSVERLIHPEGRIVGLKERVRHREGRKPAHVFEIRVN
                     SEELSKPKFRTISIDRHGYDKAFELSVDIICKERGLDKHSPIRKMMLESVYIYKGMSP
                     RDGETVLNTKDDLSDMERALREHVAAFKEKRSVIKG"
     gene            complement(3353..>3648)
                     /locus_tag="VFX11_13445"
     CDS             complement(3353..>3648)
                     /locus_tag="VFX11_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4939677.1"
                     /translation="YLAQLPLSVMKIDKIFVDDIHRPQGSAIVSAVLALARGLDVDVV
                     AEGVETADQARELKALGCRFAQGYYFAKPLGVTDVEALLMQKYARAQDRRVNP"
BASE COUNT          789 a         1054 c         1043 g          762 t
ORIGIN      
        1 cgccagtaca aacaggatca cgccaatcga gacgacatcc atgtctgctc attccttcac
       61 gagttgcgga tcaggtaatc aaatgccgcc agggaagcct tggcaccctc gcccatcgca
      121 atgatgatct gcttgtaggg aaccgtagta cagtcgcctg ccgcgaacac gccgggcacg
      181 gacgtctggc cacgatcatc cacaatgatc tcgccacgat tgctcaattc caccgtacct
      241 ttcagccagt cggtgttcgg caccagacca atctgcacga acacacccgc cacatccaca
      301 cgatgaacgt cgccactgac gcgatccttg tagatcaggc catccatctt gtggccgtca
      361 ccggtcacct cggtggtctg tgccgacagg atcaccgtga tattgttcat gctgtacatt
      421 tttttctgca gtatggcgtc cgcgcgcagc ttgctgtcga attccagcac agtgacatgc
      481 tcgacgatac ccgccagatc gatggccgcc tcgatacccg agttgccgcc accaatcact
      541 gccacccgct tgcccttgaa tagcgggccg tcgcagtgcg ggcagtaggc cacgcccttg
      601 gtgcggtatt ccgtttcgcc cggcacgttc atgttgcgcc agcgggcacc ggtggaaagg
      661 atcaccgtct tgctgcgcag cgaggcgccg ttttcaaact tgatatggac catgccatca
      721 cccgactggc tggccggcac caacgcggtc gcacgctgat tgttcatgat gtccacgttg
      781 tagtgcttga cgtgggcttc cagagcgcca gccagcttgg gcccttcggt ttccagcacg
      841 gaaatgaagt tttcaatggc catggtatcc atcacctggc cgccgaaacg ctctgctgcg
      901 acgccggtgc ggatgccctt gcgcgcagcg taaatggccg ccgacgaccc cgacggaccg
      961 ccgcccacca ccagcacatc gaacgcttct ttctgctcca gcttggccgc ttcacgggca
     1021 gccgcaccgg tatcgatctt gccgagtatt tcttccagcg tcatgcggcc ctggccgaac
     1081 ggttcaccgt tcaggtacac cgcaggcacg cccatgatct cgcgctgatt cacttcatcc
     1141 tggaacagcg cgccatcgat catcacatgg ctgatgttcg gattcagcac cgccagcagg
     1201 ttcaatgcct gcaccacatc gggacagttg tggcaggtca gcgaaataaa ggtttcaaaa
     1261 ttgaatttgc cgtcgagcga cttgatctgt tcgatcactt ccggctcgac tttcggtgga
     1321 tgcccgccca cctgcaacag ggccagcacc agcgacgtga attcgtggcc catcggaatg
     1381 ccggcaaagc gcacgctgcc catgttggca ccggcgcgat acagcgcgaa cgagggcttg
     1441 cgctccttgt catcgcggct cacattcagc ttgatcttgt cggtcagccc cacgatgtcg
     1501 ttcagcaggt ccagcatttc ctgcgaatgg gcactgtcat caacggatgc gttgatctcc
     1561 aacgggtaaa caacccgttc cagatacgcc tgcaactgac ctttgagtgt tgtgtccagc
     1621 atgttcgact cctgagattg actcggactg aattaatcag ttttgctcac ccggcagcaa
     1681 atgccgggca taaaaagccc cgccgtcagg cagggcttcc atgtgtgcct gaaagaactg
     1741 tccgtcgccg gatcagatct tgccgaccag atccagagaa ggagcgatgg tcttggcgcc
     1801 ttctttccac ttggccgggc agacctggcc aggatggctg gcaacgtact gggctgcctt
     1861 gagcttgcgc aaggtttcag atacatcgcg tgcaatcgcg ttgtcatgca cttccagggt
     1921 cttgatcacg ccttccgggt tgatgatgaa ggtgccacgc agtgccagac cttcttccgg
     1981 aatgtgtacg ccgaaggcgt tggtcagttg gtgagtcggg tcgcccacca gcgggaactt
     2041 ggccttgcct actgcaggcg aggtttcgtg ccacacttta tgggagaaat gggtgtcggt
     2101 ggtgacgatg tagacttcag cgccggcttt ctggaattcg gcgtagttgt cggcagcgtc
     2161 ttccacttcg gtggggcagt tgaaggtgaa ggcagccggc atgaaaatca ggacagacca
     2221 cttgcccttc agggactgct cggtaacttc aacgaacttg ccattgtgga aagcctgggt
     2281 cttgaacggt tgaacctggg tattgatcag tgacattttg aagtatctcc ttggtttgtg
     2341 gtcaggtacg gtgcaaagca taaggatctt gtggctttca aacaaattga atgttttttg
     2401 aggatggata gccaaaagct attgctcaca aagcagtcaa ttgaatccgg gccggccatg
     2461 ggatggtaac aaaatccggc caggagccgg gagaaatgaa tctggaaagg gggccacggg
     2521 aggaatgtgg caacggactg gtgcccggga ccggaatcga accggtacga gggtcacccc
     2581 tcgagggatt ttaagtccct tgcgtctacc tgtttcgcca cccgggcaat ggacaagcga
     2641 aaaaaaagaa gggaggatta tagggagggg ttctttctgg atcaacggaa tattcgccgg
     2701 ttgaaaagta cggcccgttg atagacttta gtaaagcaac ccaatcgtac atccaccggg
     2761 aatcctgaac gcatggcgat atcagtcagt gaagactaca ttcgtgttta ccgcgcctgg
     2821 aatggcaagg aataccaggt ttactacccg ctgaagaaga atggccgttt cctgacggaa
     2881 gaggaattgc tggaaacgct ggaggaagcc caggccaagg acgtcgagct ggcgcagcgc
     2941 cagcgtgcgc acttcatgcg caaggatctg tccgtggaga ggctgattca cccggagggc
     3001 cgcatcgtgg gcctgaagga gcgcgtccgg caccgggaag gccgcaagcc ggcccacgtg
     3061 tttgaaatcc gggtcaattc ggaagagctg agcaagccca agttccgcac catctccatc
     3121 gaccggcacg gctacgacaa ggctttcgaa ctgtcagtgg acatcatctg caaggagcgc
     3181 ggcctggaca agcactcccc gatccgcaag atgatgctgg agagcgtcta catctacaaa
     3241 ggcatgtcgc cgcgggatgg cgagacggtg ctgaacacca aggacgatct gagcgacatg
     3301 gagcgggccc tgagggaaca cgttgccgcg ttcaaggaga aacggtcggt catcaagggt
     3361 tgacacgccg gtcctgggcg cgggcgtatt tctgcatcag cagcgcttca acatcagtaa
     3421 ctcccagggg ttttgcaaag tagtagccct gggcaaagcg gcaaccgagc gccttgagtt
     3481 cccgggcctg atcggcggtt tcaacgccct ccgccaccac gtccacatcc agcccccgcg
     3541 ccagtgccag cacagccgag acaattgcac tgccctgggg gcggtggatg tcgtcgacaa
     3601 agatcttgtc gatcttcatg acactcaacg gtaactgcgc cagatagc
//
LOCUS       DASUEG010000713         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_354155, whole genome shotgun sequence.
ACCESSION   DASUEG010000713 DASUEG010000000
VERSION     DASUEG010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354155"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFX11_13450"
     CDS             <1..984
                     /locus_tag="VFX11_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4939678.1"
                     /translation="AYGTGVTAQRLIDQRPAKIFFTGSARTGKKILAQAADKLIPVDL
                     ELGGKDQAIIFDDVNIQRAAKGVLWGALTNAGQSCSSVERIYVQEGIYDQFVQTLQTE
                     IRKLVVNAGDTGNADMGGMTVDFQLDIVRKQVDDARAKGARILTGGDLLCDNELFYMP
                     TLLTHVDDSMLVMQQETFGPLLPVLRFSSEDEVIRLSNATEFGLSASVWSKDAARAER
                     VARQLECGAVSINNVMLTEGNPGLPFGGTKASGYGRQKGAEGLLGYTRSKSVLIDGDS
                     AKIEPNWYPYTQQKYNLFSQLIDALFTASPWRLLKAALIGMKLEAEAKKER"
     gene            1025..1507
                     /locus_tag="VFX11_13455"
     CDS             1025..1507
                     /locus_tag="VFX11_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEX4939679.1"
                     /translation="MIFSHSGQYTGQAITLEAAMISANAVPHTQLNDREDRLSAAALL
                     NFFGITDEWGLTSKEQMVLLGNPAKTTFYRMKDYADGKAEKPVRLSQDTLERVSYIMG
                     IYKAINILLPTNRRAAEWIKLPNTAGLFGGQSALDKMLQGRVSDLCDVRRYLDAERGH
                     "
     gene            1507..2247
                     /locus_tag="VFX11_13460"
     CDS             1507..2247
                     /locus_tag="VFX11_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX4939680.1"
                     /translation="MTAILRDEPLQEVWLDGPQYRIIPTRFPPINFFERYTPPELMEA
                     AFEIESMTNERLREEAGELHRVAPEDRVSGPGAGTVMAAFTHTGNPSRFTNGSFGIYY
                     CARELETAIRETVFHRERFLGATQQTACEIEMRVFKGTLEKPLLDVRGAEFSHLLNPD
                     PDAYAASQAFGNDIKQRNAWGVVYPSVRNVQGQCVAAFRPPAVSLPVQSKLLAYHWNG
                     KRICRVYEKKEILLELDHEQEEDNVFLL"
     gene            complement(2395..>2942)
                     /locus_tag="VFX11_13465"
     CDS             complement(2395..>2942)
                     /locus_tag="VFX11_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4939681.1"
                     /translation="LCTMFYHLAKHPEWQERLRAESRALGKEQLDYDDLDAMEGMTLV
                     MKEALRLCAPVPSIPRKTVKDVVYKGYLIPKGSMITVSPYFTHFMPEFWPNPTHFDPE
                     RFSEARREDKVHPYAYVPFGGGAHMCIGLHFADLQVKAVLHQILLHYRWSVPKDYKMP
                     IDMTSLPVPGDKLPVKLERID"
BASE COUNT          638 a          934 c          824 g          546 t
ORIGIN      
        1 gcctacggca ccggcgtcac cgcccagcgc ctgatcgacc agcgtccggc gaaaattttc
       61 ttcaccggca gcgcccgcac cggcaaaaaa attctggcgc aggccgccga caaactgatt
      121 ccggtggacc tggaactggg cggcaaggat caggcgatca tttttgacga cgtcaatatc
      181 cagcgtgcgg caaaaggcgt gttgtggggt gcactcacca atgcgggaca atcctgcagt
      241 tcggtggaac gcatctacgt gcaggaaggc atctacgacc agttcgtgca aaccctgcaa
      301 accgaaattc gcaagctggt ggtcaacgct ggcgacaccg gcaacgccga catgggtggc
      361 atgacggtgg atttccagct cgacatcgtg cgcaaacagg tggacgacgc ccgcgccaaa
      421 ggtgcccgca ttctcactgg cggtgatctg ctgtgcgaca acgaattatt ttatatgccc
      481 accctgctca cccatgtgga cgacagcatg ctggtgatgc agcaggaaac cttcggcccg
      541 ctgctgccgg tactgcgctt cagcagcgaa gacgaagtga tccggctgtc caacgccacc
      601 gaattcggcc tgtccgccag tgtctggtcg aaggatgcgg cgcgggcaga acgggtggca
      661 cgccagctgg agtgcggtgc cgtgtccatc aacaacgtca tgcttaccga gggcaacccc
      721 ggcctgccct tcggcggcac caaggccagc ggttacggcc gccagaaagg tgccgagggt
      781 ctgctgggct atacccgctc gaaatcggtt ctgatcgacg gcgacagcgc caagatcgaa
      841 cccaactggt atccctacac ccagcaaaaa tacaatcttt tcagccagtt gattgacgcg
      901 ctcttcaccg ccagcccctg gcgcctgctg aaagcggccc tgatcggcat gaaactggag
      961 gcggaagcga agaaagaacg ctgacgccct acgcagagac atctgccccg aatggaacca
     1021 atatttgatt ttttcccatt cggggcaata tactggacag gccatcaccc tggaggccgc
     1081 catgatttcc gccaatgccg ttccccacac ccagctcaat gaccgcgaag accgcctcag
     1141 cgctgccgcc ctgctcaact ttttcggcat caccgatgaa tggggcctga cctcgaagga
     1201 acagatggtg ctgctgggca acccggccaa gaccaccttt taccgcatga aggactatgc
     1261 cgacggcaag gcggaaaaac ccgtgcgcct gagccaggac acactggaac gggtctccta
     1321 catcatgggc atctacaagg ccatcaacat cctgctgccc accaaccggc gcgcggcgga
     1381 atggatcaag ctgcccaaca ccgccggcct gttcggcggg cagagcgcgc tggacaaaat
     1441 gctgcagggc cgcgtcagcg acctgtgcga cgtgcgccgc tacttagacg ccgagcgggg
     1501 ccactgatga ctgccattct cagggacgag ccgcttcagg aagtctggct cgacggcccg
     1561 caataccgca tcattcccac gcgctttccg cccatcaatt ttttcgagcg ctacacgccg
     1621 ccggaattga tggaagccgc gtttgaaatc gaatccatga ccaacgaacg tctgcgggag
     1681 gaagccggcg aactgcaccg cgttgcacca gaggatcgcg tctccggccc cggcgccggc
     1741 accgtcatgg ccgccttcac ccacacggga aatcccagcc gtttcaccaa tggcagcttc
     1801 ggtatttatt actgcgcccg cgaactggaa accgccattc gcgaaaccgt gtttcatcgc
     1861 gaacgcttcc ttggcgccac ccagcagact gcctgcgaaa tcgaaatgcg ggtattcaag
     1921 ggcacgctgg aaaaaccgct gctcgacgtg cgcggcgcgg aattttccca tctgctgaat
     1981 cccgatcccg acgcctacgc cgcgtcgcaa gcgtttggga atgacatcaa gcagcgcaac
     2041 gcgtggggcg tggtgtatcc gtcagtgcgc aatgtacagg ggcagtgtgt cgccgcgttt
     2101 cgcccgccgg cggtgtcgct gccggtacag agcaaactgc tggcgtatca ctggaacggc
     2161 aagcggattt gccgcgtgta tgagaagaag gaaattctgc tggagctgga tcatgagcag
     2221 gaagaagaca acgtcttcct cctgtagtga ttgcctgaag aaaacggact ggctgtattt
     2281 agtcgaatat ggacacgccg tgaacccatc cctgggggct gctccgcggc atccctgccg
     2341 cagagcgtcc atcttcgact aaatacagcc agcccgtttt cttcagcttt tttctcagtc
     2401 gatccgctcc agcttcaccg gcaacttgtc ccccggcacc ggcagcgacg tcatgtcgat
     2461 cggcatcttg taatccttcg gcacactcca gcggtagtgg agcagaatct ggtgcagcac
     2521 cgccttcacc tgcagatccg caaaatgcag gccgatgcac atatgcgcgc caccaccgaa
     2581 cggcacatag gcataaggat gcaccttgtc ctcccgacgg gcttcgctga aacgctcggg
     2641 atcgaagtgg gtcggattcg gccagaattc cggcatgaaa tgcgtgaagt aaggtgacac
     2701 ggtgatcatg gaacctttcg gaatcaggta ccccttgtac accacatcct tcactgtctt
     2761 gcgcggaatc gacggcaccg gcgcgcacag ccgcagcgct tccttcatca ccagcgtcat
     2821 gccttccatt gcatccagat catcgtaatc cagctgttcc ttgcccaaag cccggctttc
     2881 cgcccgcagc cgctcctgcc actccggatg cttggccaga tggtagaaca tggtgcacag
     2941 cg
//
LOCUS       DASUEG010000714         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_185586, whole genome shotgun sequence.
ACCESSION   DASUEG010000714 DASUEG010000000
VERSION     DASUEG010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185586"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFX11_13470"
     CDS             <1..880
                     /locus_tag="VFX11_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4939682.1"
                     /translation="QPQVALLTAQRNRQSAALYSNEQDSFIKHRLSVLSELRQAIARE
                     QLSIYIQPQLRVRDQLLVGGEVLVRWHHDRDGYISPGVFIPLAEQSQLIFDITRQVLL
                     QSCQWLQRQQSLPQDFHLSVNLSALDINDRRLLPFIEYCMTEYHVDRRRILFEVTESA
                     VMDDPERFLGVIRQLHELGFKIAIDDFGTGYSSMSYLQQMNADEIKIDIRFVRNIHLS
                     PTNQNIVKAIVQLARATHAYTVAEGIETEAELNMLRALECNIAQGFLWSAAVPAEEFS
                     QRFLAGSRPVNLQQSS"
     gene            1004..1603
                     /locus_tag="VFX11_13475"
     CDS             1004..1603
                     /locus_tag="VFX11_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939683.1"
                     /translation="MHTAFSKKSALAAALLAAALSVPAQATSVSVTTDGSWYQFDVDE
                     VVAGSGGLEWIDAITDGHGLYSGDGSALSFTFTLNQAGTLSIVDGGFGGDEYQVSVNG
                     VNYFTSTPLAPGLASTGTDFDAALANTDGFSYLNLFLNPGTYTVTGLLQVSASDPYGN
                     PYNASVGGLRVSEVPVPAAAWLLGSGLLALAARLRRRRA"
     gene            1635..3455
                     /locus_tag="VFX11_13480"
     CDS             1635..3455
                     /locus_tag="VFX11_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX4939684.1"
                     /translation="MKLQLKKIAAGVALTLAGATAAQAANVTVKVIAFNDFHGQLESP
                     GSLRVNAASATPTVAVGGADYFAGHIARLKAQNPNSVVVSAGDLIGATPLVSALFHDE
                     PTIEAMNLAGLEFNAVGNHEFDEGKDELKRMQNGGCHPTDANSCKGLQTGTPYPFSGA
                     KFKFLAANVEETATGKAIFPPYAIKTFGKTRVAFIGMTLKDTPSIVTPSGVAGLAFRD
                     EAATVNALVPKLRARGVEAIIVLIHQGGTVPGTQAADTINSCAGGLAGTPMPPIVSAL
                     DDEVDMVISGHTHQAYNCQLANSTGRLIPVTSANSIGRVLSDINLTLNDANGEVVSIS
                     ANNILVDRTHSATTPNAQIAGLVSQYKALATPIANRVIGEITSAMPHNAATAAGEIAL
                     GDVLADAQLAATAPAGFGEAVAAFMNPGGIRSPGFTFPSSAAGEGDGKVTYGEAFTVQ
                     PFGNSLVTLSLTGEQIHIMLEQQFIGCHAGTAPASWNYPASDTSGQPFNRILQVSEGF
                     RYAWSASGPACDKVDPTSISINGATLDLNASYRITVNNFLADGGDKIYVLAQGNNRLG
                     GAQDVDALEDWFNNPAADADPVASGVQVAPGPQDRIMLLP"
     gene            complement(3558..4037)
                     /locus_tag="VFX11_13485"
     CDS             complement(3558..4037)
                     /locus_tag="VFX11_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939685.1"
                     /translation="MATLLAMCLLLVMGSCRAQPPVEPSAANGALALVAAAIDGVRNG
                     IAVTDFARERVLALQPGTAGNFQAEQSGTRLVLFQNDAGTLVRADLYFLLADAPRRAE
                     LETLTGALTLYQQSKDSRARAELAATPTQAGAVLFVELLGAQPDGSTPVKSLSARVR"
     gene            complement(4060..4743)
                     /locus_tag="VFX11_13490"
     CDS             complement(4060..4743)
                     /locus_tag="VFX11_13490"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4939686.1"
                     /translation="MPKVVVASYDAMGGPEVAAMASDGYTAGPTDAGEYRILYCGQHS
                     SRAYASWSKILWGSELKDDGAGNLLVKHQGKWQSLGKLTPVTRKEIAEYHEALYGTKK
                     VPDKWVFNDFGHITCYFYKDKNRDGKFNKDSESIHAEFFHTTPVNEAQQAQGLPVILE
                     NSHGCIHVKPLDIDGMRKKGYLNKGNRVVIHGYAEKTVAFANDAKGRSPFEVHFYPGL
                     NQIKIVGVR"
     gene            complement(4841..5227)
                     /locus_tag="VFX11_13495"
     CDS             complement(4841..5227)
                     /locus_tag="VFX11_13495"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4939687.1"
                     /translation="MDFDAQPVAGSHGNALTERGVAQLTEYFAGQRQHFDLPLAARGT
                     SFQRSVWQALQRIPFGTTCSYQDIADTIGNPKAVRAVGAANGCNPIPIIVPCHRVIGR
                     NGSLIGFGGGLPRKEWLLRHEGALLV"
BASE COUNT         1052 a         1677 c         1512 g          995 t
ORIGIN      
        1 ccagccccag gtggcactgc tgacagccca gcgcaaccgg cagtcagcgg cgctctattc
       61 caacgagcag gacagtttca tcaagcaccg gctgtccgtg ctgtcggaat tgcggcaggc
      121 catcgcgcgg gagcagttga gcatctatat ccagccgcag ctgcgggtgc gcgaccagtt
      181 gctggtgggc ggcgaggtgc tggtgcgctg gcaccatgac cgcgatggtt atatcagccc
      241 cggcgtgttc atcccgctgg cggagcaaag ccagctgatc ttcgacatca cgcgccaggt
      301 gctgctgcaa agctgccagt ggctgcaacg gcagcaatcg ctgccacagg attttcatct
      361 gtcggtgaac ctgtcggcgc tggacatcaa tgaccgccgc ctgctgccct ttatcgaata
      421 ctgcatgacg gaataccacg tcgaccgccg ccgcattctg ttcgaggtga ccgaatccgc
      481 cgtgatggat gatccggaac ggtttctggg cgtgatccgt cagctgcatg agctgggctt
      541 caaaattgcc atcgacgatt tcggtaccgg gtattcgtcg atgtcgtacc tgcagcagat
      601 gaacgcggac gagatcaaga tcgatatccg cttcgtgcgc aatatccatc tgtcgcccac
      661 caaccagaac atcgtgaaag ccatcgtgca gctggcacgg gcaacccacg cctacacggt
      721 ggcagaaggc atcgaaaccg aagcggaact gaacatgctg cgggcgctgg agtgcaatat
      781 cgcgcagggc tttctgtggt cggcggcggt gccggcagag gagttctcgc agcggttcct
      841 ggcgggcagc cgccccgtca acctgcaaca atccagctaa aaatacgcaa cattcccgtc
      901 acccccactt cacacaaata ttccattctg ccccaggtcc tgcccgctgg gcacgacgtc
      961 gcctgttcga ggcatttctt gagacccacg gggagacacc atcatgcaca ccgcattttc
     1021 gaaaaaaagc gcccttgccg ccgcactgct ggccgctgcg ctcagcgttc cggcccaggc
     1081 caccagcgtc agcgtcacca ccgatggcag ctggtaccag ttcgacgtgg acgaagtcgt
     1141 cgccggcagc ggcggcctgg aatggatcga cgccatcacc gacggccatg gactctatag
     1201 cggtgatggt tccgcgctga gcttcacctt caccctgaat caggccggca ccctgtccat
     1261 cgtcgatggc ggtttcggcg gtgatgagta tcaggtcagc gtcaatggcg taaattattt
     1321 cacctccact ccactggcac ccggactggc cagcacaggc acggatttcg atgcggccct
     1381 ggcaaacacc gacggcttca gctacctcaa cctgtttctg aatcccggca cctacaccgt
     1441 gacaggactg ctgcaggttt cggccagcga tccctacggc aatccctaca acgcgtcggt
     1501 cggcggcctg cgggtgagtg aagtgccagt gcccgccgcc gcctggttac tgggttccgg
     1561 cttgctggct ctggctgccc gcctgcgccg tcgtcgcgcc tgatttgcat ctattacacg
     1621 gggagaaaaa attcatgaaa ctgcaactca agaaaattgc ggctggcgtc gcactcacac
     1681 tggctggcgc cacagcggcc caggccgcca acgtcacggt caaggtcatc gcgttcaacg
     1741 attttcacgg ccagctggaa tcgccaggct ctttgcgggt caacgcggcc agcgcaacac
     1801 ccactgtcgc tgtcggcggc gcggattatt ttgccggcca cattgcgcgt ctgaaagcgc
     1861 agaatccgaa ttcggtggtg gtgtccgccg gcgacctgat cggcgccacg ccgctggtgt
     1921 cggcactgtt ccatgacgaa cccaccatcg aggcgatgaa tctggccggc ctggaattca
     1981 atgcggtggg taaccacgaa ttcgatgaag gcaaggacga actcaagcgc atgcagaacg
     2041 gtggatgtca ccccacggat gccaacagtt gcaaaggctt gcagacaggc acgccctacc
     2101 cgttcagcgg tgcgaaattc aaattcctcg ccgccaacgt ggaggaaacc gccaccggaa
     2161 aagccatttt cccgccctat gccatcaaga cttttggtaa aacccgcgtc gcctttatcg
     2221 gcatgacatt gaaggacacg ccgagcatcg tcacgcccag cggcgtggcc ggactggcat
     2281 tcagggatga ggccgccacc gtcaacgcac tggtaccgaa actgcgcgcc cggggcgttg
     2341 aagcaatcat tgtgctgatc caccagggtg gcactgtgcc aggcacccag gccgccgaca
     2401 ccatcaacag ctgtgccggt ggtctggcgg gcacaccaat gccccccatc gtgagcgcgc
     2461 tggatgatga ggtggacatg gtgatctccg gtcacacgca tcaagcctac aactgccagc
     2521 tggccaacag caccggccgt ctgattccgg tgaccagcgc caactccatc ggccgggtgc
     2581 tgtcagacat caatctgacc ctcaacgatg ccaacggtga agtggtcagc atcagcgcca
     2641 acaacattct ggtggaccgc acccattctg ccaccacacc gaacgcgcag atcgccggcc
     2701 tcgtcagtca gtacaaggcg ctggccacac cgatcgccaa ccgggtgatc ggcgaaatca
     2761 cgtctgccat gccccacaat gccgcaaccg ctgccggtga aattgcgctg ggcgatgtgc
     2821 ttgccgacgc gcaactggcg gcaactgcac cggcgggttt cggtgaagca gtggcagcct
     2881 tcatgaatcc aggtggcatc cgctcccccg gtttcacgtt cccttccagt gcggccggcg
     2941 aaggcgatgg caaggtcacc tacggcgagg cctttaccgt gcagcctttc ggcaacagcc
     3001 tggtgacatt gagcctcacc ggcgagcaga tccacatcat gctggaacag cagttcatcg
     3061 gttgccacgc gggcactgca ccagcgtctt ggaactatcc tgccagcgac accagcggcc
     3121 agccgttcaa ccggattctg caagtgtcgg aaggtttccg ttatgcatgg agcgcgtcgg
     3181 gcccggcctg cgacaaggtg gaccccacca gcatcagcat caatggcgcc acgctggatc
     3241 tgaacgccag ctaccgcatc acggtgaata atttcctggc agatggcggc gacaagatct
     3301 atgtgctggc acagggtaat aaccgcctgg gtggcgcgca ggatgtggat gcactggaag
     3361 actggttcaa caacccggcg gcggatgctg atccggttgc cagcggcgtg caggtggcgc
     3421 cggggccgca ggaccggatc atgctgctgc cctgatcgca ggttgacgta gtaactgaga
     3481 aagaatcccg ccggcgctaa ccggcgggat ttttgtttat ggaaagaaat gccgtaccgc
     3541 tgcagcgagg agcgtgatca gcgcaccctc gccgacaggg atttcaccgg agtgctgcca
     3601 tccggctgtg cgcccagcag ctccacgaac agcactgcgc cggcctgcgt cggggtggct
     3661 gcaagttctg cccgcgcccg gctgtccttc gattgctggt acagcgtcag cgcaccggtc
     3721 agcgtttcca gctccgcgcg gcgcggcgcg tctgccagca gaaaatacag gtccgcccgc
     3781 accagcgtgc cagcgtcgtt ctggaacagc accagccgcg tgcccgactg ttctgcctgg
     3841 aaattaccgg cggtaccggg ttgcaacgca agaactcgct cgcgagcaaa atcagtaacc
     3901 gcaataccgt tgcgcacacc gtcgatggcc gctgccacca gcgccagcgc gccatttgcg
     3961 gcggacggtt ccaccggggg ctgcgcgcgg caactgccca tcaccagcaa cagacacatt
     4021 gccagcaggg ttgccatccg gtacctcatt tctcgcatat caacgcactc ccacaatctt
     4081 gatctggttc aggccgggat agaaatgcac ttcaaatggc gaccggccct tggcatcgtt
     4141 agcgaaggcc acggtttttt ccgcatagcc atgaatcacc acgcgattac ccttgttcag
     4201 ataacccttc ttgcgcatcc cgtcgatatc cagcggttta acatgaatgc agccatggga
     4261 attttccaga atgaccggca atccctgcgc ctgctgcgcc tcgttcaccg gtgtggtgtg
     4321 aaagaattcg gcatggatgg attcggaatc cttgttgaat ttgccgtcgc ggtttttgtc
     4381 cttgtaaaaa tagcaggtga tatggccaaa gtcgttgaac acccatttgt caggcacttt
     4441 tttggtgccg tacaaggctt catggtactc cgcgatctct ttgcgcgtga ccggtgtgag
     4501 cttgcccaga ctttgccact tgccctggtg tttcaccagc aggttgccgg caccgtcgtc
     4561 cttgagttcg gaaccccaca ggattttcga ccaggaggca taggcccggc tggaatgctg
     4621 gccacaatac aggatgcggt attcgccggc atcggtcggc cccgcggtat aaccatcgga
     4681 cgccatcgcc gccacttccg gcccgcccat tgcgtcgtaa cttgccacta ccaccttcgg
     4741 cattcgcctg ctcccccttc atcggtcagt caaggattta acaccttccc gcaacaaccg
     4801 gcacggcatt ccggcacact tccgcgatga attgctgcat ctagaccagc aacgccccct
     4861 catgccgcag cagccattcc ttccgcggca gtccgccgcc aaatccgatc agcgaaccat
     4921 tgcgcccgat cacccggtgg cagggcacga tgatgggaat gggattgcag ccgttggcgg
     4981 cacccacggc gcgcacggct ttgggattgc cgatggtgtc ggcgatatcc tggtaggaac
     5041 aggtggtgcc aaagggaatc cgctgcagcg cctgccacac gctgcgctgg aaggacgtgc
     5101 cccgcgccgc gagcggaaga tcgaaatgct ggcgctggcc cgcaaagtac tccgtcagct
     5161 gggcaacgcc gcgttcagtc agtgcattgc cgtgactgcc cgccaccggt tgcgcgtcaa
     5221 aatcaatcgc ggtgat
//
LOCUS       DASUEG010000715         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1512146, whole genome shotgun sequence.
ACCESSION   DASUEG010000715 DASUEG010000000
VERSION     DASUEG010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512146"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFX11_13500"
     CDS             <1..296
                     /locus_tag="VFX11_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4939688.1"
                     /translation="SRQLAKAFKALSNPNRLQIFQEIMRRRQLDSKDVCGCALTDFIN
                     SLNVGAPTVSHHIKELVNADLIRVERNGKFITCFLNEDMSLRLKEFFAAPEQQ"
     gene            386..1645
                     /locus_tag="VFX11_13505"
     CDS             386..1645
                     /locus_tag="VFX11_13505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4939689.1"
                     /translation="MIRGYVHPAFAGVASELARQIPDNGRSGAAVAVYHQGHCVVDVW
                     GGCRDREGNPWQEDTLSLSFSTTKGVASTLLHVLADQGLIDYHKPVSSYWPEFAQSGK
                     GDITVLHLLCHQAGLYDIRGMIDDATRITDWEYMTDTLAAAAPVHRPGEAHGYHGITY
                     GWLVGELIQRVTGKSFADVLKSELAEPLGLDGLYVGLPEDQFYRRAYLPNFPRSHRPP
                     AEANARKRPTSLQNRLQANLIRGAIQMIGVDPDDFTRGLAPHGMARFSFNDDSVVKAC
                     IPAANGMFTARSLARLYAVMANDGELDGVKLMSPQRVREISQIQSRKVDRVVPIPMHW
                     RLGYHRVFTTGPRTPHAFGHFGFQGSGAWCDPTRHLAVGFIVNTSGGTTPFGDDRIAR
                     INAQAIRAAEKVEGVKSPRFGLVPFLN"
     gene            complement(1695..1943)
                     /locus_tag="VFX11_13510"
     CDS             complement(1695..1943)
                     /locus_tag="VFX11_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX4939690.1"
                     /translation="MQSPRKKENDRPVSKRKILPALLLCFFFGIFGTHRFYVGKVNSG
                     ILMMLTLGGLGFWVIFDLLVLLLGRFCDNEGAVLKDWT"
     gene            complement(1912..2532)
                     /locus_tag="VFX11_13515"
     CDS             complement(1912..2532)
                     /locus_tag="VFX11_13515"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX4939691.1"
                     /translation="MPTKATQQTPTRCLWCGDDPLYQAYHDEEWGVPVRDDRRMFEFI
                     VLEGAQAGLSWITILRKRENYRAAFDQFDPEKIARYTPARIEKLLLNPGIVRNRLKVE
                     GTVRNARAYLNILEKQGSFSDYLWQFVDGEPVQNNRASMKDVPATTPQSDQMSKALKK
                     AGFTFVGSTICYAHMQATGMVNDHTVDCFRHQECRKLAKPAQKRKR"
     gene            3003..3743
                     /locus_tag="VFX11_13520"
     CDS             3003..3743
                     /locus_tag="VFX11_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939692.1"
                     /translation="MRTDVSENMQPYVIIVEDDERLADLTAEYLRSNGIKVDVIHDGS
                     VAIDAIIDNQPDMVVLDLMLPGADGLEVCKSVRDRYKKPILMLTARTDDVDQVLGLEM
                     GADDYVAKPVKPRVLLARIRALLRRSDPAEKQSDKGGADQGHNRLEFGDLVIDNSARE
                     VWLNGEAVDLTSAEYDLLWLLASNAGTILSREEIFERLRGIQYDGQDRSIDVRVSRIR
                     PKVGDDPMNPRRIKTVRSKGYLFVRDLT"
     gene            3757..5379
                     /locus_tag="VFX11_13525"
     CDS             3757..5379
                     /locus_tag="VFX11_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939693.1"
                     /translation="MNSIFLRIYGGMILVCVLIGVLFYVSLETINFFRLNLFRANLVS
                     GPIELVAEMTAKQLPDYQARWVEDVGRLLDARMQLRERSELALDEADIEQLQKGKLVL
                     RMLDDFNRQGEAVVKVPGELDRYIVARGEYLTEQQARGVAELVAQHLSAHPVLQWNEL
                     LEKLNRQFGFSLKRVPPFQVELDDEKKHRLFNDEQAVATFSGNRGGNTQITAYKLLTE
                     TGEVLMLGPQQLFNWYPFELMAVLVVFALAFVGFGVYMLVKPLETRLSGLEKAVLRIR
                     GGDMNARAPVEGADAIGQLAGTLNGMTEHIQRLLNSQKELTRAVSHELRTPVARIRFG
                     LEMMIDAMNDEQRLKAADAIDQDIEQLDKLIDEILTYSKLEEGTPVLDFVMIDVDQLL
                     RRLEQESQALAGKKQVVYMAPDLPEERRFAEGEERYVHRVVQNLVGNALRYARSRVVI
                     SYGVMGDVFRIDVEDDGPGIPESDRKRVFQPFTRLDDSRTRASGGYGLGLSIVERIAY
                     WHGGRARVAESSMGGAKFSFLWPRLQSLRESN"
     gene            complement(5425..>5705)
                     /locus_tag="VFX11_13530"
     CDS             complement(5425..>5705)
                     /locus_tag="VFX11_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939694.1"
                     /translation="TVQQRLAEGFTDIRLARRIYPQWHYPLDEHELVELFRHKFGPVK
                     RAFDAGTPAQQTSLREKLAEVYRNNSERKNGILTITGGEYLEVIATRR"
BASE COUNT         1140 a         1538 c         1801 g         1226 t
ORIGIN      
        1 actcgcggca actggccaag gccttcaagg ccctgtccaa ccccaaccgg ttacagatct
       61 ttcaggaaat catgcggcgg cggcagctgg actcgaagga cgtgtgcggc tgcgcattga
      121 ccgacttcat caattccctg aatgtggggg cgcccaccgt gtcgcaccat atcaaggaac
      181 tggtgaatgc cgacctgatc cgggtagagc gcaatggaaa gttcattacc tgttttctca
      241 atgaagacat gagcctgcgg ctgaaggaat ttttcgcggc gcccgaacaa caataaaaag
      301 ctgcggcgtt ttgcaatttt ggtacgacat tgattgttta aaacattggc actgcagcga
      361 cactgcagtt acagaggaaa gtcagatgat cagaggctat gtgcatccgg cgtttgccgg
      421 tgtcgccagc gagctggccc gccagattcc cgacaacggc cgcagtggcg cggcggtggc
      481 ggtgtaccac cagggacatt gtgtggtgga tgtgtggggc ggctgtcgcg accgtgaagg
      541 caatccctgg caggaagaca cgctgtcgtt gtctttttcc accaccaagg gtgtcgcgtc
      601 cacgttgctg catgtgctgg cggaccaggg cctgatcgat taccacaagc cggtgagttc
      661 ttactggccc gaattcgccc agtccggcaa gggcgacatc accgtgctgc atctgctctg
      721 ccaccaggcc ggtctgtacg atattcgcgg catgatcgac gatgccaccc gcatcaccga
      781 ctgggaatac atgaccgaca ccctggccgc cgccgcaccg gttcaccgtc cgggcgaggc
      841 gcacggttat cacggcatca cgtacggctg gctggtcggt gaattgatcc agcgggtgac
      901 cggcaaatcc ttcgcagacg tattgaagag cgaactggcg gaaccgctgg gtctggacgg
      961 actgtacgtt ggcttgccgg aagaccagtt ttaccgccgc gcttacctgc ccaattttcc
     1021 ccgcagccat cgtccgccag cggaagcgaa cgcgcgcaag cgtcccacca gtttgcaaaa
     1081 tcgtctgcag gccaacctga ttcgcggagc catccagatg attggagtgg acccggatga
     1141 tttcacccgc ggactggcac cccatggcat ggcacgcttc agtttcaatg acgattcggt
     1201 tgtgaaggcc tgcattcctg ccgccaatgg catgttcacg gcccgctcgc tggcgcggtt
     1261 gtatgcggtg atggccaacg acggtgaact ggacggcgtc aaactgatgt cgccacaacg
     1321 ggtgcgcgag atcagccaga tccagagccg caaggtcgat cgcgtagtgc cgattcccat
     1381 gcactggcgg ctgggttatc accgggtatt caccaccggt ccgcgcacgc cccatgcgtt
     1441 cgggcatttc ggttttcagg ggtccggtgc ctggtgcgat ccaacccggc atctggcggt
     1501 gggttttatc gtcaacacca gtggtggcac cacgccgttc ggcgatgatc gcatcgcccg
     1561 catcaatgcg caggcgatcc gggcggcgga aaaagtggaa ggcgtgaagt caccgcgctt
     1621 cgggctggtg ccgttcttga attgagatca gacaggtaaa gggatgcgag ggccggtggc
     1681 tgacggccgt gccctcaggt ccagtctttc agtaccgcgc cttcgttgtc gcagaagcgt
     1741 cccagcagca gtaccagcag atcgaagata acccagaacc ctaggccacc gagggtcagc
     1801 atcatcagaa taccgctgtt gaccttgccc acataaaagc ggtgagtgcc gaaaatcccg
     1861 aagaagaagc acaacaacag cgcgggcaga atttttcgtt tggaaacggg cctatcgttt
     1921 tcttttttgc gcgggctttg caagttttct gcactcctgg tggcggaagc aatccaccgt
     1981 gtgatcgttc accatgccgg tcgcctgcat gtgggcatag cagatggtgc tgccgacaaa
     2041 cgtgaaaccg gctttcttca gcgccttgct catctggtcc gactgcggtg tggtggcggg
     2101 cacatctttc atgctggccc tgttgttctg caccggttcg ccgtcgacga actgccacag
     2161 gtagtccgag aaactaccct gtttctccag aatgttcaaa taggcgcggg cgttgcgcac
     2221 cgtgccttcc accttcaggc gattgcgcac gatgccggga ttcagcagca gcttttcgat
     2281 gcgggcgggg gtgtagcggg cgatcttttc gggatcgaac tggtcgaagg cggcgcggta
     2341 gttttcgcgc ttgcgcagga tggtgatcca gctgaggccc gcctgggcgc cttccagcac
     2401 gatgaactcg aacatgcgcc ggtcgtcgcg caccggtaca ccccattcct cgtcgtggta
     2461 ggcctgatag agcggatcgt ctccgcacca caggcagcgc gtcggagttt gctgagtggc
     2521 tttggtgggc atgcgggcgt tccctgaagt gaaggcacaa aaaaagcccc gcgatcgcgg
     2581 ggacgatgcg gggccatatt agcgcggttt tgacggttat agttattgtg ggtgtagacc
     2641 ggtgctcccc ctggggttgt ggatcgttgt tggttattca caggccaaaa atggccccgg
     2701 caggctgggt ggcccgccac aatatctggg tgtttgtttg ttgttgatat acaagatagt
     2761 gcgcacccgc tggggtcgca aggcgatttt tcggggataa cttgtggata aaatgtggga
     2821 atgatgtgtt atgtggaatt tttgcctagc tcatttagat gccgctggta taccctattg
     2881 gcgcctattt gtgcaaagtc aggcagatgg actggcagac tttgtgtaaa caacctatct
     2941 ttttatttaa gttgctaaaa aaacaacaaa tttgttccgg accccggtaa gaggacgttc
     3001 tgatgcgcac agacgtaagt gaaaacatgc agccctacgt catcattgtt gaagacgacg
     3061 agcggctggc tgatctgaca gcggaatacc tgcgcagcaa tggcatcaag gtggacgtca
     3121 ttcacgacgg cagcgtggct atcgatgcga tcatcgacaa ccagcctgat atggtggtgc
     3181 tggatctgat gttgccgggt gcggatggac tggaagtctg caagagtgtg cgcgaccggt
     3241 acaagaagcc gatcctgatg ctgaccgccc gtaccgatga tgtggaccag gtgctggggc
     3301 tggaaatggg ggccgacgac tacgtggcga aaccggtaaa accccgggtg ctgctggccc
     3361 gcatccgtgc gttgctgcgg cggtccgatc cggcagaaaa gcaatccgat aaaggcggcg
     3421 ccgaccaggg ccacaaccgg ctggagtttg gtgatctggt aattgacaac agtgcgcggg
     3481 aagtctggct caatggcgag gccgtggatc tcaccagcgc ggaatatgat ttgctgtggc
     3541 tgctggcgtc gaatgccggc accatcctgt cccgcgagga gattttcgaa cgcctgcggg
     3601 gcatccagta cgacggccag gaccgctcca tcgacgtccg cgtgtcgcgc atccgtccca
     3661 aggtgggtga tgatcccatg aatccgcgcc gcatcaagac tgttcgcagc aaaggttacc
     3721 tgttcgtgcg cgacctcacc tgaacggcgg gtctgattga acagcatatt ccttcgtatt
     3781 tacggtggca tgatcctggt ctgtgtcctg atcggggttt tgttttacgt atccctcgaa
     3841 accatcaatt ttttccgtct gaatctgttc cgcgccaatc tggtgtccgg gcccattgaa
     3901 ctcgtcgctg aaatgacggc aaaacagctg ccggattatc aggcgcgctg ggtggaggac
     3961 gtcggccgtc tgctggatgc gcgcatgcag ttgcgcgagc gcagcgagct ggcactggac
     4021 gaggccgata tcgaacagct gcagaaaggc aagctggtgc tgcgcatgct ggacgatttc
     4081 aatcgccagg gcgaggcggt ggtgaaagtg ccgggtgaac tggaccgcta cattgtggcg
     4141 cggggcgaat acctgaccga gcagcaggcg cgcggcgtgg cggagctggt ggcgcagcat
     4201 ctgtcggcgc atccggtgtt gcagtggaat gaactgctgg aaaaactcaa ccggcaattc
     4261 ggattttccc tcaagcgcgt gccgccgttt caggtggaac tggatgacga gaaaaaacac
     4321 cgcctgttca atgacgagca ggccgtggcc actttttccg gcaatcgcgg cggcaacacc
     4381 cagatcacgg cgtacaagct gctgacggaa accggcgaag tactgatgct gggaccgcag
     4441 cagttgttca actggtatcc gttcgaactg atggcagtgc tggtggtgtt tgccctggcg
     4501 ttcgtgggtt ttggtgtgta catgctggtg aagccgctgg aaacccgttt gtctggcctg
     4561 gaaaaagcgg tgttgcggat tcgcggtggt gatatgaatg cgcgggcgcc ggtggaaggg
     4621 gctgatgcca tcggtcagct ggcaggtacc ctgaacggaa tgaccgaaca catccagcgc
     4681 ctgctgaatt cgcagaagga attgactcgc gccgtgtcgc acgaactgcg tacgccggtg
     4741 gcgcgcatcc gcttcggcct ggaaatgatg atcgacgcga tgaacgacga gcagcgcctg
     4801 aaagcggccg acgccatcga tcaggacatc gagcagctgg ataaactgat tgacgagatt
     4861 cttacctatt ccaagctgga agaaggcacg ccggtactgg actttgtgat gatcgacgtg
     4921 gaccagctgt tgcgccgcct ggagcaggaa agccaggccc tggccggcaa gaagcaggtg
     4981 gtgtacatgg cgccggatct gccggaagaa cgccgctttg ccgagggcga ggaacgttat
     5041 gtgcaccgcg tggtgcagaa cctggtgggc aacgcgttgc gttatgcccg cagcagggtg
     5101 gtaatcagtt acggtgtaat gggcgacgtg ttccgcatcg atgtggagga cgacggcccc
     5161 ggcattcctg aatcggatcg caagcgggtg ttccagccgt tcacccgtct ggatgacagc
     5221 cgcactcgcg cgtccggtgg ttatggattg gggctttcca ttgtggagcg gattgcgtat
     5281 tggcacggcg gccgtgcccg tgtggccgaa agcagcatgg gcggcgcgaa attcagtttc
     5341 ctgtggccgc ggttgcagag tttgcgggaa tcgaactgaa gggcgtgcgg gacgcccttt
     5401 tttgcgagca gattatttgt ctggctagcg gcgtgtcgcg atcacttcca ggtattctcc
     5461 gcccgtgatg gtcaatatgc cgttcttcct ctcactgttg ttgcggtaaa cctcagccag
     5521 cttttcacgc agggaagtct gttgtgccgg agtcccagca tcaaacgcgc gcttgaccgg
     5581 tccgaacttg tggcggaaca gctccaccag ttcgtgttcg tcgagtggat agtgccattg
     5641 cgggtaaatc ctgcgggcga ggcggatatc ggtgaatcct tctgccagac gttgctgcac
     5701 tgtca
//
LOCUS       DASUEG010000716         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_227287, whole genome shotgun sequence.
ACCESSION   DASUEG010000716 DASUEG010000000
VERSION     DASUEG010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227287"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1132)
                     /locus_tag="VFX11_13535"
     CDS             complement(161..1132)
                     /locus_tag="VFX11_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX4939695.1"
                     /translation="MTDSRLAIVGVTGEVGRAVLSRLEDSDLAFAEVHALASSRSEDE
                     TVMFRQRPLIVESADGFDFSRADFVVLATPASVSLRLAEQALAAGCRVVDHSSAYTDQ
                     ASVPLAGTPDAESADLVSVPMAEVSLLAPLLGALAGEAGLRVAHVTMLLPVSSSGHAG
                     VKELAGQTGELLNGRGIEPSVFPVQTAFNTLPLVGSEREDAVTEGLQQLLGEHVPLVL
                     SSVIVPVFYGLTAQVTLETEVPLLSGAAIDLLSRAEGVDVKNSNEDQQVATPITDAAG
                     QSAIYVCGVRELPAPLCGITLTAVADNVHQGAARRTVDLLEKWIKRL"
     gene            complement(1201..2307)
                     /gene="asd"
                     /locus_tag="VFX11_13540"
     CDS             complement(1201..2307)
                     /gene="asd"
                     /locus_tag="VFX11_13540"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX4939696.1"
                     /translation="MKVGLVGWRGMVGSVLMQRMVEENDFAHIDPVYFSTSNAGGKAP
                     DFGGKAAPALQDANSVDALKGMDVIITCQGGDYTNDIYPKLRAAGWNGYWIDAASALR
                     MEDDAVIVLDPVNDNVIRSALAKGVKNYIGGNCTNSIMLMGMGGLFREGLVDWVSSMT
                     YQAASGGGANHMRELLKGMGVIHAAVADELATPSSAILEIDRKVAKTIREDVPTEFFG
                     APLAGGLIPWIDKQLDNGQSKEEWKGQAEVNKILGTASTIPVDGLCVRIGAMRCHSLA
                     LTVKLKKDLPLSEIEAIIKSGNDWVKFVPNDREISVKELTPASITGGLKIGVGRVRKL
                     NMGPEYISAFVIGDQLLWGAAEPLRRMLRILLGK"
     gene            complement(2341..3414)
                     /gene="leuB"
                     /locus_tag="VFX11_13545"
     CDS             complement(2341..3414)
                     /gene="leuB"
                     /locus_tag="VFX11_13545"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX4939697.1"
                     /translation="MSKHIVILAGDGIGPEIMAEAEKVLSAVNEKFGLGFTWEHQLLG
                     GAAIDAHGVPLPDVTLDAARKADAVLLGAVGGPKWDTIERSIRPERGLLKIRSELNLF
                     ANLRPAILYPQLASASSLKPEIVAGLDILIVRELTGGIYFGQPRGVRTLENGERQGYN
                     TDVYSESEIRRIAHVAFQLAMQRNKKVLSVDKANVLEVTELWKEIATEVGKEYPEVQL
                     SHMYVDNAAMQLVRAPKQFDVIFTGNLFGDILSDEAAMLTGSIGMLPSASLDENKKGM
                     YEPCHGSAPDIAGKGVANPLATILSVAMMLRYTFGQEVAAKCIEDAVGKVLDQNLRTP
                     DIYTEGTTKVGTKEMGAAVVAAL"
     gene            complement(3473..4120)
                     /gene="leuD"
                     /locus_tag="VFX11_13550"
     CDS             complement(3473..4120)
                     /gene="leuD"
                     /locus_tag="VFX11_13550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX4939698.1"
                     /translation="MEKFTVVKGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFD
                     EWRYLDEGYPGQDNSKRPVNPDFVLNFPRYQGASVLIARQNFGCGSSREHAPWALQEY
                     GFRSVIASSFADIFFNNCFKNGVLPVVLKEEEMDVLFKECEASEGYQITVDLEQKKVI
                     RPNGESFSFEVDDFRRHCLLNGLDDIGLTLQVADDIRAFEEKAKVARPWVFQSLR"
     gene            complement(4132..>4742)
                     /locus_tag="VFX11_13555"
     CDS             complement(4132..>4742)
                     /locus_tag="VFX11_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX4939699.1"
                     /translation="LVSDADATFDAVVKIDGAAILPQVSWGTSPEMVVPVTATVPTLA
                     DAKDDVQRNSFTRAYQYMGLEAGQKITDIHLDRVFIGSCTNSRIEDLRAAAAVIKGRK
                     VASTIKQAMVVPGSGLVKKQAEAEGLDKLFLAAGFEWREPGCSMCLAMNADKLQPGEH
                     CASTSNRNFEGRQGNGGRTHLVSPAMAAAAALAGHFVDVRSF"
BASE COUNT          978 a         1444 c         1376 g          944 t
ORIGIN      
        1 gcttacgcag gaccattatc ggattccttt attggttatt ctttcgccct tcaaaaacac
       61 ggtggacgtt accgagtggc caagattccg gccttttccc atgggtacta agactactat
      121 cctagaattt gtatcacaag atattcgtaa gtaccaataa ttaaagtctt tttatccact
      181 tttcaagcaa gtccaccgtg cgacgggcgg ctccttgatg gacgttgtcg gccactgccg
      241 taagggttat cccgcacaag ggcgccggca actcgcgaac accacagacg tatatcgcag
      301 actgacctgc cgcatccgtt atcggcgtcg caacctgctg atcttcattg gaattcttga
      361 catccacccc ctctgcgcga gagagcaggt cgatggcggc acccgacaac aacggcacct
      421 ctgtctcaag ggttacctga gccgtcaggc cgtaaaaaac cggtacgatc acagacgaga
      481 ggaccaaagg cacgtgttca cccagcaatt gctgaagccc ctcggtgacg gcatcctccc
      541 gttccgaccc gacgagcggc aaggtattga aggcggtctg caccgggaag acggaaggct
      601 cgatgccccg gccattgagc aactctcccg tctggccggc caactctttc acaccggcat
      661 gaccagacga cgacactggc aacaacatgg tgacatgggc aacacggagc cccgcctccc
      721 ccgcaagagc ccccaacagg ggcgccaaca gcgacacttc agccatcggt acgctaacca
      781 ggtctgcact ttccgcatcc ggagtgcctg ccagcggcac acttgcctga tccgtataag
      841 ccgatgagtg atcgaccact cgacaccccg ccgccaacgc ctgttcagcc agtcgcaatg
      901 aaacggatgc tggtgtcgcc agcacaacaa aatcggcacg ggaaaaatcg aagccatccg
      961 ccgattccac gatcagcggg cgctgccgga acatgaccgt ttcatcttcg gaacgggacg
     1021 aggccagtgc atgcacttcg gcaaatgcca gatccgagtc ttccaggcgc gacaggactg
     1081 cgcgccccac ttcaccggta acacccacga tggccagacg agaatcagtc atgtttcatc
     1141 aactccagaa aatgcaaaac ccgcccgcgc cggattggca cgggcgggca tggtacaccc
     1201 ttacttgccg agcaggatgc gcagcatgcg acgcagcggt tcggcagcac cccagagcag
     1261 ctggtcgcca atcacgaagg cggaaatgta ttccgggccc atgttcagct tgcgcacgcg
     1321 accgacaccg atcttgaggc cgccggtgat ggacgccggg gtcagttcct tgacgctgat
     1381 ctcgcgatcg ttcgggacga acttcaccca gtcgttgccg gacttgatga tggcttcgat
     1441 ctcggacagc ggcaggtcct tcttcagctt cacggtgagc gccaggctgt ggcagcgcat
     1501 cgcgccgatg cgcacgcaga ggccgtcaac cggaatggtg ctcgcggtgc caaggatctt
     1561 gttcacttcg gcctggccct tccactcttc cttggactgg ccgttgtcga gttgcttgtc
     1621 gatccagggg atcagaccac cggcgagcgg cgcaccgaag aattcggtcg gcacgtcttc
     1681 gcgaatggtc ttggctacct tgcgatcgat ctcaaggatc gcggaggacg gggtcgcgag
     1741 ttcgtcggcc acggcggcat gaatcacacc catgcccttg agcagttcac gcatgtggtt
     1801 ggcaccgcca ccagaagcgg cctggtaggt catggagcta acccagtcga ccaggccttc
     1861 gcggaacagg cctcccatgc ccatcagcat gatggagttg gtgcagttac cgccgatgta
     1921 gttcttgacg cccttggcca gcgccgagcg aatgacgttg tcgttcaccg gatcaagcac
     1981 gatgacggca tcgtcctcca tgcgcagcgc ggaggcggca tcgatccagt aaccgttcca
     2041 gccagcagca cgcagcttgg gatagatgtc gttggtgtag tcaccgccct ggcaggtgat
     2101 gatcacgtcc atgcccttga gagcatcaac ggaattggcg tcctgcagcg caggagcggc
     2161 cttgccaccg aaatccggag ccttgccacc ggcattactg gtggagaaat acaccggatc
     2221 gatgtgggcg aaatcgtttt cttccaccat gcgctgcatg aggaccgaac ccaccatgcc
     2281 acgccaacct accagaccga ctttcatgtt cattaccttt tcaaaagact gaatgcgttt
     2341 ttacagtgct gcgacaacgg ctgcgcccat ttccttcgtg ccgaccttgg tcgtgccttc
     2401 ggtataaatg tccggcgtac gcaggttctg gtcgagcacc ttgccgacgg catcttcgat
     2461 gcacttggcg gcgacttcct gaccgaaggt gtaacgcagc atcatggcca ccgagaggat
     2521 ggtggcgagc ggattggcca cgcccttacc ggcgatgtcc ggtgccgaac cgtggcaagg
     2581 ctcgtacatg cccttcttgt tttcgtccag cgaggccgat ggcagcatac cgatggaacc
     2641 ggtgagcatg gcagcttcgt cagaaagaat gtcgccgaac aggttgccgg tgaagatcac
     2701 gtcgaactgc ttgggcgcac gcaccagctg catggcggca ttatcgacat acatgtgcga
     2761 gagctggact tccggatatt ccttgcccac ttctgtcgca atttctttcc agagttcggt
     2821 cacttccagc acattggctt tgtcgaccga cagcactttc ttgttgcgtt gcatggcgag
     2881 ctggaacgcc acgtgggcaa tgcggcggat ttccgattcg ctgtacacgt cggtgttgta
     2941 accctgacgc tcgccgttct ccagcgtgcg cacgccgcgc ggctgaccaa agtaaatgcc
     3001 gccagtgagc tcacgcacga tcaggatatc cagacccgcc acgatttccg gcttcaggga
     3061 agaagcggac gccagttgcg gatacaggat ggccggacgc aggttggcga acaggttgag
     3121 ttcgctacgg atcttcagca ggccacgttc aggacgaatg gaacgttcga tggtgtccca
     3181 cttcgggcca ccgacggcac cgagcagaac ggcatcggct ttcctggcgg catcaagcgt
     3241 gacatccggc agcggcacgc cgtgtgcatc aatggcggca ccgcccagca gctggtgttc
     3301 ccaagtgaaa cccaagccga atttttcatt gacggccgac agcacctttt cggcttctgc
     3361 catgatttcc gggccgatgc cgtcgccggc gagaatgaca atatgcttgc tcattgtttc
     3421 tgaatccttg tggttcgccc tgccccgcgc cagcggaaca ggggttaata catcaacgca
     3481 gcgactggaa cacccaggga cgggcgacct tcgccttctc ttcaaaggca cgaatgtcat
     3541 cggccacctg cagggtgaga ccgatgtcgt cgagaccgtt cagcaggcag tggcggcgga
     3601 aatcatccac ctcgaaactg aaggattcgc cgttcgggcg gatcactttc ttctgctcca
     3661 ggtcgacggt gatctgatag ccctcgctgg cctcgcactc cttgaagagc acatccattt
     3721 cttcttcttt caacaccacc ggcagcacgc cgttcttgaa gcagttgttg aagaaaatat
     3781 cggcaaagct ggaggcgatg acggaacgaa agccgtattc ctgcagggcc caaggggcat
     3841 gctcgcgcga ggagccgcaa ccgaagttct ggcgggcaat cagcacgctg gcgccctggt
     3901 agcgcggaaa attcagcacg aaatccggat tcaccgggcg cttgctgttg tcctgtcccg
     3961 gatagccctc gtccagatag cgccactcat caaacaggtt gggaccaaag cccgtgcgct
     4021 tgatggactt gaggaactgc ttgggaatga tctggtcggt gtcgacgttg gcgcgatcga
     4081 gcggagcgac gagacctttt actacggtga atttttccat gacggtctcc ttcagaacga
     4141 acgcacgtcg acgaaatgac cagccagcgc ggcagcagcg gccatggccg ggctgacgag
     4201 atgggtgcgg ccgccattgc cctggcgtcc ctcgaaattg cggttcgagg tggaggcgca
     4261 gtgctcgccc ggctgcagtt tgtccgcatt catggcgagg cacatggagc agcccggctc
     4321 acgccattcg aaaccggcag cgaggaaaag tttgtcgagg ccttcagctt cggcctgctt
     4381 tttcaccaga ccggagcctg gcaccaccat ggcctgcttg atggtgctgg cgaccttgcg
     4441 gcctttgatc acggcggcag cggcgcgcag gtcctcaata cgggaattgg tgcaggaacc
     4501 gatgaacacc cgatccagat ggatgtcagt gatcttctgg ccggcctcca ggcccatgta
     4561 ctggtaagcg cgggtgaagg agttgcgctg cacgtcgtcc ttcgcgtcgg cgagggtcgg
     4621 cacggtggcg gtgaccggca ccaccatttc cgggctcgtg ccccaggaca cctgcggcag
     4681 gatggcggcg ccgtcgatct tcacgactgc gtcgaaggtc gcgtcggcgt cggagaccag
     4741 gg
//
LOCUS       DASUEG010000717         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2195757, whole genome shotgun sequence.
ACCESSION   DASUEG010000717 DASUEG010000000
VERSION     DASUEG010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195757"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFX11_13560"
     CDS             <1..420
                     /locus_tag="VFX11_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016971678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4939700.1"
                     /translation="GTYGYFKEHALCKGDFKTNVNEQPGSASVVQSVAGSVNSIGYSG
                     IGYTTSSVKAVPLAKKAGADFVAATAEKALTGEYPLSRALYIYVNKAPGKELQPLERE
                     FIKMVLSKQGQETVVKDGYIPMPAEMAAKALAKLGIK"
     gene            588..>2651
                     /locus_tag="VFX11_13565"
     CDS             588..>2651
                     /locus_tag="VFX11_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4939701.1"
                     /translation="METTSESMFGLSAQRMARLRYLRKLKDRIATATITLGGISIIFA
                     ICLIFFYLLYEILPLFMPASIDERSTLNSLQVSDPYDVMLEEQTMIGLVIDRQQGPLF
                     FDARTGAAVAHGATLPASPITAFSKDAPSSRAFALGFADGGVLLLQHDYDAHYEGDTK
                     VLDPKLKFPMGETPLPVLQGSPVKQLAVRDAGDVLKIAALDDQNRIFLKVFEKVESFM
                     SDEVTLEEQDTLQLPTASAPVDQILMSLDQRWLFLLENRNQIEIFDLRKTPDQMRVGL
                     VHVTEGEARITRAEFLLGGFSLLLADDQGRIAQWFMARDRKDGSDILAHVRTLELEPG
                     VAITSLQSEHRRKGFVAGDANGNVGFFNATAERLALDEKITTGALEWIAMAPRGDAIL
                     LKGVGQQALNFAVDNEHPEVSWSSLWGEVWYERYEEPDYIWQSSSANNDNEPKFSLMP
                     VTFGTLKAAFYAMLVAIPLSICGAIYTAYFMAPRLRTKVKPTIELMGAVPTVILGFLA
                     GLWLAPLVEANLPGMFSILILTPVLCVITALVWSQLPLRVRTFIPDGWVPLILAPVVV
                     LSGWISMEISEPLEHMFFGGNMRQWLTHDLGIDFDQRNSMIVGIAMGFAVIPSIFSIA
                     EDAIFAVPKSLTQGSLALGATPWQTLVRVILPTASPGIFSALMIGLGRAVGETMIVLM
                     ATGNTP"
BASE COUNT          483 a          775 c          835 g          558 t
ORIGIN      
        1 ggcacctacg gttacttcaa ggagcacgcg ctgtgcaagg gcgacttcaa gaccaacgtc
       61 aatgagcagc ccggttctgc gtccgtggtg cagtcggtgg ccggttcggt caacagcatt
      121 ggttactccg gtattggtta caccacctcc agtgtgaaag ctgtgcccct ggccaagaag
      181 gccggcgccg atttcgtggc cgccaccgct gaaaaggccc tgaccggtga atacccgctg
      241 tcccgtgccc tgtacattta tgtgaacaag gcgccgggca aggagctgca gccgctggag
      301 cgcgagttca tcaagatggt gctgtcgaag cagggccagg aaaccgtggt gaaggatggt
      361 tacatcccga tgccggcgga aatggcggcc aaggcgctgg ccaagctggg catcaagtaa
      421 gatttctgtc cggtggccgc tctgccgggc ttgagtcgac aactggggct gcattagagt
      481 gcggcccccg tttttttgtt aaaagagcgg ttttttgtta gaaagccgtt tttgcaagcg
      541 cacgccgcca gccggtgttt gtcctatcag agtgagtacc acagcttatg gaaacgacca
      601 gcgaatcgat gttcgggcta tcggcccagc ggatggctcg gcttcgctac ctgcgcaagc
      661 tcaaggaccg gatcgccacg gccaccatca cgctcggtgg catcagtatc atctttgcga
      721 tctgcctgat cttcttctat ctgctgtatg aaatcctgcc gttgttcatg ccggcgtcga
      781 tcgatgagcg cagcaccctc aacagtctcc aggtaagcga tccctacgac gtgatgctgg
      841 aagagcagac catgatcggt ctggtcatcg atcgtcagca ggggccgctg ttctttgatg
      901 cccgcaccgg tgccgccgtg gcccatggtg ccacgctgcc ggcatcaccc atcactgcct
      961 tttccaagga tgcaccttcg tcccgcgcgt ttgcgctggg gtttgccgat ggtggcgtgc
     1021 tgctgctgca gcacgattac gacgcccact acgagggcga caccaaggtg ctcgacccga
     1081 aactgaaatt ccccatgggg gaaacaccgc tgccggtact gcagggttcg ccggtgaagc
     1141 agctggcggt gcgggacgcc ggcgacgtgc tgaagatcgc ggcgcttgat gaccagaacc
     1201 gcattttcct caaggtgttc gaaaaggtcg aaagcttcat gtccgacgaa gtgacgctgg
     1261 aggagcagga tacgttgcag ttacccacgg cgtcggcacc ggtggaccag atcctgatga
     1321 gtctggacca gcgctggctg ttcctgctgg aaaaccgcaa ccagatcgag atctttgacc
     1381 tgcgcaagac gccggaccag atgcgggtgg gcctggtgca tgttaccgag ggcgaggccc
     1441 gcatcacccg tgctgaattc ctgctgggcg gtttttcatt gctgctggcg gatgaccagg
     1501 gccgcatcgc ccagtggttc atggcgcgcg acaggaaaga cggctccgac attctggccc
     1561 atgtgcgaac gctggagctg gagccggggg tcgcgatcac cagcctgcag tcggagcacc
     1621 gtcgcaaggg ctttgtggcc ggtgatgcca atggcaatgt gggctttttc aatgccaccg
     1681 ccgaacgtct ggcgctggac gaaaaaatca ccacgggcgc gctggagtgg atcgccatgg
     1741 cgccgcgggg ggatgccatc ctgctcaagg gcgtgggtca gcaggcattg aattttgcgg
     1801 tggacaatga gcacccggag gtgtcctggt cgtcgctgtg gggagaagtc tggtacgagc
     1861 gctacgagga accggactat atctggcaaa gctcctccgc caataacgac aacgagccca
     1921 agttcagcct gatgccggtg accttcggca cgctgaaagc ggcgttctac gccatgctcg
     1981 tggccattcc gctgtcgatc tgcggcgcga tctacaccgc ctacttcatg gcaccccgtc
     2041 tgcgcaccaa ggtcaagccc accattgagc tgatgggggc ggtgcccacc gtgatcctgg
     2101 gttttctcgc cggcctgtgg ctggcgccgc tggtggaagc caatctgccc ggcatgttct
     2161 cgatcctgat cctgacgccg gtgctgtgtg tgattaccgc gctggtctgg tcgcagctgc
     2221 ctctgcgcgt gcgcacgttt attcccgatg gctgggttcc cctgatcctg gcgccggtgg
     2281 tggtgctgtc cggctggata tccatggaga tcagcgaacc gctggagcac atgttctttg
     2341 gcggcaacat gcgccagtgg ctgacccatg atctgggtat cgactttgac cagcgcaact
     2401 ccatgatcgt gggcattgcc atgggtttcg cggtgatccc cagtattttc tccatcgccg
     2461 aggatgcgat ttttgcagtg ccgaaaagct tgacgcaggg ttcgctggcc ctgggagcca
     2521 caccctggca gaccctggtg cgggtgatcc tgcccactgc cagccctggc attttttccg
     2581 cgctgatgat cggtctggga cgtgccgtgg gtgaaaccat gatcgtgctg atggccaccg
     2641 gcaacacgcc g
//
LOCUS       DASUEG010000718         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1333762, whole genome shotgun sequence.
ACCESSION   DASUEG010000718 DASUEG010000000
VERSION     DASUEG010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333762"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFX11_13570"
     CDS             <1..987
                     /locus_tag="VFX11_13570"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4939702.1"
                     /translation="GLCSHEYFHSWNVKRIAPAIPYDLAQENHTPLLWAFEGITSYYD
                     DLVLVRTGLISPADYLDMLGKTITRILRNPGRLQQSVADSSFDAWTKFYKQDENSANA
                     IVSYYTKGSLVALGLDLLLRQVSDQQVTFDDVMRDLWQRHGLTGIPVQEHDIQHTCAR
                     LLREKNAAPAALESLQRFCEHAIYASEELQLEEWLRSAGVEYALRKSASMSDSGGSAG
                     KNADRRPDLGIGTSGAGHGASVTRVLNGSAAHRAGISAGDVLIAIDNVRVDNSSLEKV
                     LNRYQPGDVIDVHGFRRDELMQFRVTLAEPALDTCYLTIIDEDTVRRWLQPQ"
     gene            complement(1003..1833)
                     /locus_tag="VFX11_13575"
     CDS             complement(1003..1833)
                     /locus_tag="VFX11_13575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4939703.1"
                     /translation="MLKFLAILLFVLTANSLRAETRDLDHGIRLQGQFVQGGMVTGTA
                     PADVTVQFQNRTLPRASDGLFVLGFNRDDPPEQQLVLIDAKGRKQSVLFSIRQRAYEV
                     QKVNGVPPRTVNPDPKDMARIERETAQVAQAREAVSTQPFFSKGFRWPVKGPVSGVYG
                     SQRIFNGEPRSPHFGVDLAVPAGTPILAPADAVVTLAEKDLFFSGGTIILDHGMGINS
                     GYLHMSKLLVKKGDKVKQGQKIGLVGSSGRATGPHLCWRLNWYQSRLDPQLVTATPKP
                     "
     gene            complement(1845..>2036)
                     /locus_tag="VFX11_13580"
     CDS             complement(1845..>2036)
                     /locus_tag="VFX11_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX4939704.1"
                     /translation="LTLEQVKALQSALNAAGYAAGVADGVLGSGTKKSLRAYQRANGM
                     IADGYPAPDVFKSLGVVYP"
BASE COUNT          415 a          641 c          605 g          375 t
ORIGIN      
        1 ggcctgtgca gccacgaata ttttcacagc tggaacgtga agcggatcgc gccggcgatt
       61 ccctacgatc tggcacagga aaaccacaca ccactgctgt gggcatttga aggcatcaca
      121 tcgtattacg acgatctggt actggtgcgc accggcctga tcagccccgc cgactatctc
      181 gacatgctgg gcaaaaccat cacccgcatc ctgcgcaatc ctggccggct gcagcaatcg
      241 gtggcggatt ccagcttcga cgcctggacc aagttctaca agcaggatga gaacagcgcc
      301 aacgccatcg tcagttacta caccaagggc tcgctggtgg cactggggct ggatttgttg
      361 ttgcgccagg tgagcgacca gcaggtgacg ttcgacgacg tgatgcgtga tctgtggcaa
      421 cgccacggcc tgaccggcat tccggtgcag gaacacgaca tccagcacac ctgcgcgcgc
      481 ctgctgcggg aaaaaaatgc cgcaccggcc gcgcttgaat ccctgcagcg tttttgcgag
      541 cacgcgatct atgccagcga ggagctgcaa ctggaagagt ggttgcgttc cgctggcgtg
      601 gaatacgcgc tgcgcaaatc agcctcgatg agtgacagcg gcggcagcgc gggcaagaat
      661 gcggacaggc ggccggatct gggcatcggc accagcggtg ccggccacgg cgccagtgtg
      721 acgcgggtgc tgaacggcag tgccgcgcac cgcgccggga tttcggcagg ggatgtgctg
      781 attgccatcg acaacgtgcg ggtggacaac agctcgctgg aaaaagtgct gaaccgctac
      841 cagcccggcg acgtcatcga tgtgcacggt ttccgccgcg acgaactgat gcaattccgt
      901 gtaacgctgg cggagccggc actggatacc tgttacctca ccatcatcga tgaagacacc
      961 gtgcggcgct ggttgcagcc tcagtaagag acacttgaac aatcagggtt tgggtgtcgc
     1021 ggtcaccagt tgcgggtcca gccgcgactg gtaccagttc aaacgccagc acagatgggg
     1081 accggtggcg cgaccgctgg aacccaccag accgatcttc tgcccctgct tcaccttgtc
     1141 gccctttttc accagcaatt tgctcatgtg cagataaccg ctgttgatgc ccatgccgtg
     1201 atcgagaatg atggtgccac cggaaaagaa cagatccttt tccgccagcg tcaccacagc
     1261 atcggcaggc gccaggatgg gagtgccggc gggcacggcg agatccactc cgaaatgcgg
     1321 gctgcgcggc tcgccattga aaatgcgctg actgccgtac acgccgctca ctggcccctt
     1381 caccggccag cggaatccct tgctgaaaaa gggctgcgtg gagaccgctt cccgtgcctg
     1441 cgccacctgc gcggtttccc gctcgatccg cgccatgtct ttcggatcgg gattcaccgt
     1501 gcgcggcggc acgccattca ctttctgcac ctcgtaggcg cgctggcgga tgctgaacag
     1561 cacgctctgc ttacgcccct tcgcatcgat gagcactagc tgttgctccg gcggatcatc
     1621 gcgattgaat cccagcacaa acaagccatc gctcgcccgt ggcaaggtac ggttctggaa
     1681 ttgcacggtt acgtcggcgg gcgctgtgcc ggtcaccatc ccaccctgca cgaactgacc
     1741 ctgcaaccgg atgccatggt cgagatcgcg ggtttcggcg cggagtgaat ttgcggtgag
     1801 cacaaacaac agaatcgcta agaatttcag cacactcgat ttcctcacgg ataaaccaca
     1861 cccagcgact tgaacacgtc cggcgccgga taaccatcgg caatcatgcc gtttgcgcgc
     1921 tgataggcgc gcagggattt tttggtgccg gagcccagca caccgtccgc cacgccggca
     1981 gcataaccgg ctgcattcaa cgcagactgc agcgccttca cctgttccag cgtcag
//
LOCUS       DASUEG010000719         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1555045, whole genome shotgun sequence.
ACCESSION   DASUEG010000719 DASUEG010000000
VERSION     DASUEG010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555045"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFX11_13585"
     CDS             <1..1233
                     /locus_tag="VFX11_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939705.1"
                     /translation="PADCVPVLSTDPLYILYTSGTTGKPKGVVRDNGGHAVAMKYSMK
                     AVYDIAPGDVFWAASDVGWVVGHSYIVYAPLIAGATTVLYEGKPIKTPDAGAFWRVCA
                     EYRVKALFAAPTAFRAIRKEDPDSRCLQGHDLSALRCVFVAGERLDPPTYHWVKDTLG
                     KPVVDHWWQTETGWAICGNLTGVELKTPKPGSSTVPTPGFHVQVLDDDGHPLPPGKQG
                     SIVVKRPLPPSCLPTIWGDFARFQNDYLKAFPGYYLTGDGGYIDDEGYVFIMGRIDDV
                     INVAGHRLSTGEMEEILAGHPAIAECCVIGVHDELKGQVPVGLVLLKNGVAIDQQQLE
                     AELSSCIRQEIGAIANFRKAFVVPRLPKTRSGKILRKLLRQIAAGDDVAIPSTIDDPA
                     SIPEIAGLMAQHGLIQKR"
     gene            complement(1264..1974)
                     /locus_tag="VFX11_13590"
     CDS             complement(1264..1974)
                     /locus_tag="VFX11_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008958125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4939706.1"
                     /translation="MHTHSRQIFDCGDAALPAEVLEPAQPATACVIWLHGLGASGDDF
                     VPVPPHLKLPDNLAVRFIFPHAPDMPVTCNNGYIMPAWYDILALTEIREINRQQLQAS
                     CARIATILQQQIAQGIAPERCLLIGFSQGGAVAYHTALTFPHKLAGLIALSTYMPAPE
                     LVQQADLSANRTLPIRIAHGDADDMVTERASRRAFDWLQQQGYDVDWKRYPMAHELCV
                     GEIRQIGEWVRTMLPAGQ"
     gene            complement(1982..2320)
                     /locus_tag="VFX11_13595"
     CDS             complement(1982..2320)
                     /locus_tag="VFX11_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX4939707.1"
                     /translation="MSTLFSKIIDREIPASIVYEDDLCLAFRDINPQAPMHILIIPKK
                     PVALLADATVEDQALLGHLLLKAGDIAKKEGYGDAFRLVVNNGAKAGQTVFHLHVHIL
                     GGRGFSWPPG"
     gene            complement(2340..2555)
                     /locus_tag="VFX11_13600"
     CDS             complement(2340..2555)
                     /locus_tag="VFX11_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX4939708.1"
                     /translation="MFDTKLLEILVCPATKAKLEYDAERQELICLASNLAYPIRDGIP
                     VMLEEEARQISLDEKESYKKKYRSNGL"
     gene            complement(2624..>2811)
                     /locus_tag="VFX11_13605"
     CDS             complement(2624..>2811)
                     /locus_tag="VFX11_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX4939709.1"
                     /translation="APGKNVLEVEKKLLKHVPAEFLQDAHHWLILHGRYVCVARKPKC
                     GSCVIEDLCEFKGKMED"
BASE COUNT          485 a          785 c          933 g          608 t
ORIGIN      
        1 ccggctgatt gcgttccggt gctcagcact gatccgcttt atattcttta tacatcgggc
       61 accaccggca aacccaaagg ggtggtgcgt gacaacggcg ggcatgccgt ggcgatgaaa
      121 tacagcatga aggcggtgta tgacattgcg cccggtgatg tgttctgggc ggcgtcggat
      181 gtgggctggg tggtggggca ttcctatatc gtgtatgcgc cgctgattgc cggtgcaacg
      241 acggttctgt acgaaggcaa gcccatcaag acaccggatg ccggtgcgtt ctggcgggtg
      301 tgtgccgagt atcgcgtcaa ggcgctgttt gcggcgccga ccgcgttccg cgcgattcgc
      361 aaggaagatc ccgattcccg ctgtttgcag ggccatgatc tgtcggcact gcgctgcgtg
      421 tttgtagccg gcgagcgcct ggacccgccg acctatcact gggtgaaaga cacgctgggc
      481 aagccggtgg tggatcactg gtggcaaacc gaaaccggct gggccatttg cggcaatctg
      541 accggcgttg aattgaaaac tccaaagccg ggatcatcga ccgtgccgac gcccggtttt
      601 catgtgcagg tgctggatga cgacggccat ccgttaccgc cgggcaagca gggcagcatt
      661 gtggtgaagc gccccttgcc gcccagctgc ctgccgacca tctgggggga tttcgcccgc
      721 tttcagaacg attatctcaa ggcatttccc ggttattacc tgacagggga cggtggctac
      781 attgacgacg agggctacgt gttcatcatg ggccgcatcg acgacgtgat taacgtggcc
      841 gggcaccggc tttccacggg cgagatggag gaaattctcg ccggccaccc ggcgattgcc
      901 gagtgctgcg tgatcggtgt gcacgatgag ctgaaaggtc aggtaccggt gggactggtg
      961 ctgctgaaaa acggggtcgc cattgatcag cagcaactgg aagcggagtt atcaagctgc
     1021 atccggcagg agatcggtgc catcgccaat ttccggaaag cgtttgtggt gccgcgcctg
     1081 ccgaaaaccc ggtccggcaa aatcctgcgc aaattgttgc gccagattgc ggcgggcgat
     1141 gatgttgcga ttccttccac catcgatgat ccggccagca ttcccgagat tgccggcctg
     1201 atggcgcaac acgggttgat tcagaagcgg tgaaaggcgg gggagtttag gtgcggggtt
     1261 tggttattgg cctgctggca gcatggtccg cacccattcc ccgatctgcc ggatttcccc
     1321 cacgcacagt tcgtgcgcca tcggatagcg tttccagtcc acgtcataac cctgctgctg
     1381 cagccagtcg aatgcacgcc gcgaggcccg ttccgtcacc atgtcgtcgg cgtcgccgtg
     1441 ggcgatgcgg atcggcagtg tgcgattggc gctcaggtcg gcctgctgca ccagctcggg
     1501 agcgggcatg taggtggata gggcgatcag ccccgccagt ttgtgcggaa aggtcagcgc
     1561 ggtgtggtac gccacagcgc caccctggga aaatccgatc agcaggcagc gctccggcgc
     1621 gatgccttgc gcgatctgct gctgcaggat cgtggcgatg cgggcgcaac tggcttgcag
     1681 ttgttgccgg ttgatttcgc ggatttccgt cagcgccaga atgtcgtacc aggccggcat
     1741 gatgtagccg ttgttgcagg tgacgggcat gtcgggcgcg tgggggaaaa tgaagcgcac
     1801 ggccaagttg tcgggcagct tcaggtgcgg cggcaccggt acgaaatcgt cgccgctggc
     1861 gcccaggcca tgcagccaga tgacgcaggc ggtggcgggt tgtgcgggtt ccagcacttc
     1921 ggctggcagg gcggcgtcgc cgcaatcgaa aatctgacgg ctgtgtgtgt gcatgctgcg
     1981 ttcaacccgg cggccagctg aagccgcgtc cacccaggat gtgcacgtgc aggtgaaaca
     2041 cggtctggcc cgccttggcg ccgttgttca ccaccaaccg gaaagcatcg ccgtaacctt
     2101 cttttttcgc gatatcgccg gctttcagca gcaggtggcc cagcagggcc tgatcttcca
     2161 ccgtggcgtc ggccagcagg gcaaccggtt ttttcgggat gatcaggatg tgcatgggcg
     2221 cttgcgggtt gatgtcgcgg aacgccaggc acaggtcgtc ttcgtagacg atgctggcgg
     2281 gaatttcgcg gtcgatgatt ttgctgaaca gggtgctcat gggcgggttc cttgccgtgt
     2341 cacaggccgt tgctgcggta ttttttcttg tagctttcct tttcgtccag cgagatctgg
     2401 cgcgcttcct cttccagcat gacggggatg ccgtcgcgga tggggtaggc gaggttgctg
     2461 gccaggcaga tcagttcctg ccgctcggcg tcgtattcca gtttggcctt ggtggcgggg
     2521 cagaccagga tttcgagaag tttggtgtcg aacatggtgt attccaggag cagaaatggt
     2581 gatggttcgg gtatcgtgcc agtttggtcg tggtttctcc agatcagtct tccatcttgc
     2641 ccttgaactc gcacagatcc tcgatcacgc acgatccgca tttcggcttg cgtgccacgc
     2701 acacatagcg tccgtgcagg atcagccagt ggtgggcgtc ctgcaggaat tccgccggca
     2761 cgtgcttcag cagcttcttc tccacctcca gcacattctt gccaggggcg a
//
LOCUS       DASUEG010000720         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_781942, whole genome shotgun sequence.
ACCESSION   DASUEG010000720 DASUEG010000000
VERSION     DASUEG010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781942"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..1582
                     /locus_tag="VFX11_13610"
     CDS             362..1582
                     /locus_tag="VFX11_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939710.1"
                     /translation="MKLQTLVFLGMAVLMAGSTALAGIEYEGERNAFNRFHGKGKFTS
                     SRGEVYDGNWVDGSREGHGVFTTPAGDRYDGNWSNNQENGQGKMTWANGDVYDGNWVA
                     GKMQGEGTFTSAKGEIFKGNFSGNVRQGKGVLTTPAGEKFEGSWKGGKKSGEFRVSLR
                     DGSVATGNWVDDRAPVNAVVELADGTRYTGPVRNGVSPAGKGTCVKAGQSTPCEFKDG
                     KLVVAEVPKPEPKPAPKVEPKPAAAAAVVAAPAAVAAATPPKPVAPPVPKDPRTGRGT
                     RTDGTQFFFKHNFAAGGQSDNVPTLSVEKDLNEFGAMRISAKGGEFEVTIMVDEYVGV
                     GVYELKYFKASIQKKGEETSYRTSAAEPGRLEILKDGGGKLVGLFSFTGYPNGNVGSD
                     KRVVSEGEFVIPAP"
     gene            1844..>3005
                     /locus_tag="VFX11_13615"
     CDS             1844..>3005
                     /locus_tag="VFX11_13615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4939711.1"
                     /translation="MSKKPAGKIAPFSLRQSAGLRRSDLLSLSLLALSGVVQPGVAEE
                     ATAEKPADKPKEEVVTLDAVTVRGDAVDAAKGDANPYADETAPFKVNRSGEAKRTAPI
                     LDQPSTIMVIPKETMKATGATSMKEVMRVQPGVTLGTGEGGNTYGDRFIIRGFEARND
                     VFVDGLRDPGVLSRETFAVEQIEISKGPSSTFAGRGTTGGAVNSVTKMPKMDSFVVAE
                     LGAGTDDYKRATLDANEQLSESLAVRANVLYHDTNVAGRDEVFDERKGAALSLRYDIS
                     ADVNIVVDYYHLDTDAMPDWGIPYDLPHNRPAKVDLTNFYGQTTRDFWETQADIGTFK
                     IDWQLNDNWRLSSQTRYGETNNSYIAGAPSNPNFTAETVSSSPKNRDQDNIYV"
BASE COUNT          670 a          803 c          925 g          607 t
ORIGIN      
        1 cgatggcggt ggccatcatg ggtggtttga tcgtggccac ggcactgacc ctgctgttcc
       61 tgccggcact ctatgccgcc tggttccggg tgaaagagaa cgcctgatta cgtttccggc
      121 agaaaatccg gatattggct acgcttttgg tagtcatgca tcctaaacaa ataaattcga
      181 agcgcgggaa gtgctaacgg atgggaggga atggccgccg gtgcgacagg tgacggtagc
      241 cgtgtattta ttccactgtc tttgctataa atattaggct gattcggtaa cattctgaat
      301 aagctgcaaa atggcggaac gtgtggttgt ctcgactgtt tacactcact ggaaatatgc
      361 aatgaagctt caaaccctgg tttttcttgg aatggcagtt ttgatggccg gttcgaccgc
      421 cctggccggc attgaatacg aaggcgaacg caatgccttc aaccgttttc atggcaaagg
      481 caagttcact tccagccggg gtgaagtgta tgacggcaac tgggtggacg gcagccggga
      541 aggtcacggt gtatttacca cgccggcagg cgaccgctac gacggaaact ggagcaacaa
      601 ccaggagaac ggccagggca agatgacctg ggccaacggt gatgtgtatg acggcaattg
      661 ggttgccggc aagatgcagg gcgagggcac tttcacatcg gccaagggcg aaatcttcaa
      721 gggcaacttt tccggcaatg tgcgtcaggg caagggtgtg ttgaccaccc cggccggcga
      781 aaaattcgaa ggcagctgga agggcggcaa gaaatccggt gaattccggg tcagcctgcg
      841 tgacggcagt gtagccaccg gcaactgggt ggacgaccgc gcccccgtga atgccgtggt
      901 ggagctggcc gatggtaccc gctataccgg accggtgcga aatggtgtca gtccggctgg
      961 caagggcacc tgcgtaaagg ctggccagag cacaccgtgc gaattcaagg acggcaagct
     1021 ggtggtggcg gaagtgccga aaccggaacc caagcccgct ccgaaagtgg agcccaagcc
     1081 tgccgcagca gcggctgttg ttgctgcacc tgccgctgtg gccgccgcga ctccgcccaa
     1141 gccggtggcc cccccggtac caaaagaccc ccgtactggc cgtggcaccc gcaccgatgg
     1201 cacccagttc ttcttcaagc ataactttgc ggccggtggt cagagcgaca atgtgcccac
     1261 gctgagcgtg gaaaaggatc tgaatgaatt cggcgccatg cgcatcagtg ccaagggcgg
     1321 tgaatttgaa gtgactatca tggtggacga gtatgtgggc gtgggcgtct acgaactgaa
     1381 gtacttcaag gcctctatcc agaagaaagg agaagaaacc agctaccgca cctcggccgc
     1441 cgagccgggc aggctggaga tcctgaagga tggcggcggc aagctggtag gtctgttctc
     1501 tttcaccggc tatcccaatg gcaatgtggg cagcgacaag cgcgtggtgt cggaagggga
     1561 gttcgtcata cccgccccgt aaacgcaccg gaacagccat tccctctcaa accccgcctt
     1621 cgtgcggggt tttttgttgc tcttttgttg ccgcggactg aaaaacagtc aatcaaggat
     1681 tgacatcgga acgattaaaa ttatcattcg cgaatagtta ttatttgttt tataactcgc
     1741 ggatgttagc gagaagcatc gctcttctgc gtgagtcatt gccagcacca gcttactgcc
     1801 atcgttttta cagttgtcca ttgaccggga taggggattt gtcatgtcta aaaaacctgc
     1861 ggggaaaatt gcgcctttca gtttgcgcca gtctgccggc ctgcgccgca gcgacctgtt
     1921 gtcactgtcg ttgctggccc tgagcggcgt ggtgcagccg ggtgtggcgg aagaggcgac
     1981 cgccgaaaag ccggcggaca agccgaaaga agaagtggtg accctggatg cggtcaccgt
     2041 gcggggcgac gcggttgatg ctgcgaaggg cgacgccaat ccctatgctg acgagacagc
     2101 gccgttcaag gtgaaccgct cgggtgaagc caagcgtacc gcgccgattc tggatcagcc
     2161 cagcacgata atggttattc ccaaggaaac catgaaggct accggcgcca cctccatgaa
     2221 ggaagtgatg cgggtgcagc cgggtgtgac gctgggcacc ggcgagggtg gcaacaccta
     2281 cggcgatcgc ttcatcatcc ggggttttga ggcgcgcaac gatgtgttcg tggacggttt
     2341 gcgtgatcca ggcgtactgt cgcgcgaaac gtttgcggtg gagcagatcg aaatctccaa
     2401 gggccccagc tccacgtttg cggggcgcgg tactaccggt ggtgcggtga acagtgtcac
     2461 caagatgccg aaaatggatt cctttgttgt tgccgaattg ggtgcgggca ctgacgacta
     2521 caagcgcgca accctggatg ccaacgaaca gctgagcgaa agcctggcgg tgcgtgccaa
     2581 cgtgctgtat cacgacacca acgtggcagg tcgtgatgag gtttttgatg agcgcaaggg
     2641 cgctgcgctg tctctgcgct atgacatcag cgctgacgtc aatattgtgg tggattacta
     2701 ccacctggac accgatgcga tgccggattg gggtattccc tacgatctgc cccacaatcg
     2761 tcccgccaaa gtggacctga ccaatttcta cggccagacc acgcgggatt tctgggaaac
     2821 ccaggccgat atcggcacgt tcaagatcga ctggcagctc aatgacaact ggcgtctcag
     2881 ttcgcagacc cgttacggcg aaaccaacaa cagctacatt gccggcgcgc ccagcaatcc
     2941 caatttcacc gctgaaaccg ttagctccag cccgaaaaac cgggatcagg acaatatcta
     3001 tgttg
//
LOCUS       DASUEG010000721         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2129380, whole genome shotgun sequence.
ACCESSION   DASUEG010000721 DASUEG010000000
VERSION     DASUEG010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129380"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFX11_13620"
     CDS             complement(<1..374)
                     /locus_tag="VFX11_13620"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX4939712.1"
                     /translation="MAAKSLQQHIQRLIGFTLLVVSLAILLAVWFSTGSHVRTQISND
                     LEVGHSVLTQLLETREQQLLNSAEVLTADFGFKQAVASGDAATAQSALDNHGERIQAD
                     LMALLSLDGTVTVTTRSALATG"
     gene            complement(364..1053)
                     /locus_tag="VFX11_13625"
     CDS             complement(364..1053)
                     /locus_tag="VFX11_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEX4939713.1"
                     /translation="MPNDPGIGLQSLRLANLLITLAFVALACPQTVQADALRFQVSDG
                     GGNPLADAVILLPGAIVAAPDTLAVMDQVAKTFQPHVLVIQRGQRVNFPNSDHIRHHV
                     YSFSPAKPFEIKLYAGVPEQPLQFDTAGIVVLGCNIHDAMVGYIVVADTPLAGKTDAQ
                     GLLTLQGAPAKELRIWHPRLGSGTPQLQTLPMPAPDASGNIAIRLDIAPATSASTSST
                     GFGNRFRPHGR"
     gene            1155..2900
                     /locus_tag="VFX11_13630"
     CDS             1155..2900
                     /locus_tag="VFX11_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4939714.1"
                     /translation="MWLFGQLRWYFRAQWRRYAVAMILLTLVALLNLVPPWITGRLVD
                     AVAQGALDSARLLQQVGIIVVVACFVYVFRYLWRLSLYSASYQLGAMLRRRLYQHLLQ
                     QPQQFFQRYKTGDLMARATSDVTAVEMFAGEAMLALFDGVLTGAVILLVLVLAIDWRL
                     TLIALLPWPLMAYWFWRINNELHDSFVTSQARFSELNDRVQEAITGIRMVKAYGLEQH
                     AIDSFDQATLAAHDANMTVARSEAKYEPVIFMTVGGSFLLAIAGGAWLIHQQQLTVGE
                     LTSFTLYLGFLIWPMFAYGWLLNLLERGAVAYKRLDEIFATAPAIADSGTQELTGTPT
                     LRWVINAFHYPEREQPALADFSGELQPGHMLGIVGATASGKSTFVKLLLRQYESARAE
                     ITFDNLPLSGYRLSSLRAHIVVVPQESFLFSTSIADNIALGNPAASRQQIEDAARMAN
                     VHADILAFPEGYDTLVGEKGITLSGGQKQRIAIARALLMDAALLVLDDALSAVDVATE
                     QQILRHLQQNRRGRTTIIICHRLTAVERADHIVVLSQGRPVERGRHAELLQNAGWYAQ
                     MYHYQQIEQAVEEGR"
     gene            2916..4505
                     /locus_tag="VFX11_13635"
     CDS             2916..4505
                     /locus_tag="VFX11_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX4939715.1"
                     /translation="MFNVIVRNSRGETVSTTFISESTCTLGSQSDNQVVLSGWTVARV
                     HAELSLKEDGVYVANRARLKALRVNDSKVDAFGPLTLGDTIAIGDYHIKVQIKRGGRQ
                     QADEDSPQDMEQLHHRVREKVYWAVANALAANTSVLTADETRAAVHQLIGAAFEAFKQ
                     VPAGIDRNRVGREVMNEFVGNGALDKLLQMEDVNEIMVNAPDEIFYQTDEGTQRASER
                     FTNDDAVIQVIKRIVRESGRRIDERSPMVDARMRGGSRINAIIPPLAIKGPSLTIRRY
                     KTEKLTGSHLVNYQSLSPNMLKFLELAVKNKRNIVIAGGTGSGKSTLLNILSNFIPEH
                     ERVVTIEDAAELELSQPNLVSLETRPADVDGHGAISIRDLVRNSLRMSPDRIVVGECR
                     GGEALDMLQAMNTGHDGSLTTIHANSPRDCINRLEVLVLMSGMELPVAAIRGQIQSGV
                     HLIIQQNRFPCGSRRITSISEVSGVESNTVQLGEIFRFQQDGFDADGKVRGRFMATGY
                     IPVFLEKLQERGTKIDFSLFE"
     gene            4517..>6020
                     /locus_tag="VFX11_13640"
     CDS             4517..>6020
                     /locus_tag="VFX11_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4939716.1"
                     /translation="MSGDKSRSDVALPVNGQTGTFRRLLGYTLQHKRELTIAIALLLV
                     ATLADVAGPLLIKTFIDDYLVPGNWNFNHIGAMLVIYVVANITAAFLGFFQSLRWNSI
                     AQKVVMKLRHQIFSRVVSLPLKRFDYTPTGSLISRITNDTESVKELFVGVLGVYIQNS
                     VRVIGIFIAMAVLNWKLMLVCLLFVPAVIFVMFCYRRLSTPVFQRARELLSDINARMN
                     ETIQGVAVVQLFRQEQRFADQYHAVTEEHFSTRRRTMQMDALLLRPMVDLLHLLTLGG
                     LLYFFGHNALQNASGLIEVGVIYAFVSYLGRFTEPLIEMTQRLNLFQQAIVSAGRVFA
                     WIDEAPEQVQRKAAVSPRVGDIRFDNVSFSYDGIKPVLSGIKFHIGAGQFLGIVGHTG
                     SGKSTIASLLLRFYAPTQGGIALGGEDLAEFSQDMLRRTVAIVQQDSFIFAASVADNI
                     DIGRGLSRADIEAAAKAVGLHPHVLQLQNGYDSMLDEKGQSLSVGQRQLLS"
BASE COUNT         1182 a         1686 c         1892 g         1260 t
ORIGIN      
        1 tggccggtgg cgagggcaga gcgggtcgta acggtgacgg tgccatccag cgacagcagc
       61 gccatcagat cggcctgaat ccgttcaccg tgattgtcga gtgcgctttg cgccgtggcg
      121 gcatcgccgg aggctaccgc ctgcttgaag ccgaagtccg ctgtcagcac ttcggcggaa
      181 ttcagcagct gctgttcgcg ggtttccagc aattgggtca gcacactgtg gcccacttcc
      241 agatcattgc tgatctgggt gcgcacatgg ctgccggtgg aaaaccacac cgccagcagg
      301 atcgccagtg ataccaccag caaggtgaac ccgatcagac gctgaatgtg ttgttgcagg
      361 gatttagcgg ccatggggac ggaagcgatt gccaaagccg gtggaggatg tggatgcgct
      421 ggtggccggc gctatgtcca ggcggatggc gatattgccg ctggcatcag gtgcgggcat
      481 cggcagcgtt tgcaactgcg gtgtgccgct gcccaggcgt gggtgccaga tgcgcagctc
      541 tttcgccgga gcgccttgca gtgtcagtag accctgcgca tcggtcttgc ccgccagcgg
      601 cgtgtccgcc accacaatgt agcccaccat ggcgtcatga atattgcagc ccagcaccac
      661 aatgccggca gtgtcgaatt gcagcggctg ttccggcacg ccggcataca gcttgatttc
      721 gaacggtttc gccggggaaa aagaataaac gtgatgccgg atgtgatcgc tgttggggaa
      781 attcacgcgc tggccgcgct ggatcaccag gacatggggt tgaaacgttt tggcgacctg
      841 atccatcacg gccagtgtgt cgggcgctgc gacaatggcg cctggcagca ggatgacagc
      901 atcggcgagc ggattgccgc cgccatcgct gacctgaaag cgcaacgcat cagcctgcac
      961 cgtctgcgga caggccagcg cgacaaaggc gagcgttatc aaaagattgg caagacgaag
     1021 cgactgcaaa ccgatacccg gatcgttagg catgatagaa acaaccaaat ccatttcagt
     1081 atagcagcgg cttgcggagg cgccggaagc tgctagtatc gacagacttt attcattgca
     1141 aatcaattat ctatatgtgg ttgttcggac aattgcgctg gtatttccgc gcccagtggc
     1201 ggcgttacgc cgtcgccatg atcctgctga cgctggtggc cttgctcaat ctggttccgc
     1261 cctggattac cggccggctg gtggatgcag tggcacaggg ggcgctggat tccgcccgcc
     1321 tgttgcagca ggtcgggatc atcgtcgtgg tggcctgctt cgtctacgtg ttccgctatc
     1381 tgtggcgatt gagcctgtac agcgcgtcct accagctggg tgccatgctg cgacggcggc
     1441 tgtatcaaca tctgttgcag cagccgcagc agtttttcca gcgctacaaa accggtgacc
     1501 tgatggcccg tgccaccagc gatgtcaccg cggtggaaat gtttgcgggc gaagccatgc
     1561 tggcgctgtt cgatggcgtg ctcaccggtg cggtgatcct gctggtactg gtgctggcca
     1621 tcgactggcg cctgactctg atcgcgctgc tgccctggcc gctgatggcg tactggttct
     1681 ggcgcatcaa caatgaactg catgacagct tcgtgacatc gcaggcccgt ttcagcgagc
     1741 tgaacgaccg cgtgcaggaa gccatcaccg gcatccgcat ggtgaaagcc tatggcctgg
     1801 agcagcatgc gatcgacagc ttcgatcagg ccacgctggc ggcgcacgac gccaacatga
     1861 cggtggcgcg ctcggaagcc aaatacgagc cggtgatttt catgacggtg ggcggctcct
     1921 tcctgctggc gattgccggc ggcgcctggc ttattcacca gcagcagctg acggtgggcg
     1981 aactcacctc gttcacactg tatctgggct ttctgatctg gcccatgttt gcctatggct
     2041 ggttgctgaa tttgctggag cgcggcgccg tggcctacaa gcggctggac gagatatttg
     2101 ccacggcgcc ggcgatcgcc gacagtggca cgcaggaact gactggcact cccacgctgc
     2161 gctgggtcat caatgccttc cattaccccg agcgggaaca gccggcgctg gcggatttct
     2221 ccggcgagct gcagccgggc cacatgctgg gcattgtcgg ggccacggcg tccggcaaga
     2281 gcacatttgt caaactgttg ctgcgacaat atgaaagtgc acgtgcggaa atcacattcg
     2341 acaacctgcc attgagcggg tatcgactgt catcgctgcg ggcgcatatt gtggtcgtgc
     2401 cacaggaatc cttcctgttt tccacctcga ttgccgacaa tattgcgctg ggcaatcctg
     2461 ccgcatcgcg ccagcagatc gaagacgcgg cgcgcatggc caatgtgcac gcggatatcc
     2521 tggcgtttcc cgaagggtat gacacgctgg tcggcgagaa aggcatcaca ttgtccggtg
     2581 gccagaagca acgcattgcc attgcccgcg ccttgctgat ggatgccgcg ctgctggtgc
     2641 tggacgacgc gctgtctgcc gtggatgtgg ccactgagca gcagatcctg cgccatctgc
     2701 agcaaaaccg gcgcggacgc accaccatca ttatttgtca ccggctcacg gccgtggagc
     2761 gtgccgacca tatcgtcgtg ctgagccagg gccgtccagt cgagcgggga cgtcatgcgg
     2821 agctgctgca aaatgctggc tggtacgcac agatgtatca ctatcaacag attgaacagg
     2881 cagtggagga agggcgatga aggggcggca attccatgtt taatgtcata gtgcgcaaca
     2941 gccggggcga gacggtgtcc acgaccttta tcagcgaatc cacctgtacc ctgggttccc
     3001 agtctgacaa tcaggtggtg ctgtcgggct ggacggtggc gcgggtgcac gcggaactga
     3061 gcctgaaaga ggacggtgtt tatgtcgcca accgtgccag gctgaaagcc ctgcgcgtga
     3121 atgacagcaa ggtcgatgct ttcggtccgt tgacgctggg tgacaccatt gccatcggcg
     3181 actaccacat caaggtgcag atcaagcgtg gcggccgtca gcaggctgat gaggattctc
     3241 cccaggatat ggaacagctc catcatcggg tacgggaaaa agtctattgg gcagtggcca
     3301 atgcactggc cgccaatacc agcgttctga cagctgatga aacccgggcg gccgtgcacc
     3361 agctgatcgg cgcggcgttc gaagcgttca agcaggttcc ggccggaatc gatcgcaacc
     3421 gcgttggtcg cgaggtcatg aatgaattcg ttggcaacgg cgcgctggac aagcttttgc
     3481 agatggagga cgtcaacgag atcatggtga atgcgcccga cgagatcttt taccagactg
     3541 atgaaggcac ccagcgggcc agcgagcgct tcaccaatga cgacgctgtg attcaggtta
     3601 tcaagcgcat agtgcgtgaa tccggccggc gcatcgatga acgctcaccg atggtggatg
     3661 cgcgcatgcg cggcggttcc cgcatcaatg caatcatccc gccgttggcg atcaaggggc
     3721 cgtcgctcac cattcgccga tacaagaccg aaaagctgac cggatcgcat ctggtcaact
     3781 accagtccct gtcaccgaac atgctgaagt ttctcgagct ggcggtgaaa aacaagcgca
     3841 atatcgtgat tgccggcggc accggatcgg gcaagtccac gttgctcaat atcctgtcga
     3901 atttcattcc cgagcacgag cgggtagtca ccatcgaaga tgccgccgaa ctggagctgt
     3961 cgcagcccaa tctggtgtcg ctggaaaccc ggcctgcgga tgttgacggc cacggtgcca
     4021 tcagcattcg cgatctggtg cgcaacagtc tgcgcatgag ccccgaccgc atagtggtag
     4081 gggaatgccg cggcggtgag gcgctggaca tgctccaggc catgaatacg ggacacgatg
     4141 gttcccttac caccattcat gccaattctc cgcgcgattg catcaaccgg ctggaagtgc
     4201 tggtactgat gtccggcatg gaattgccag tggcagcgat ccggggtcag atccagtcgg
     4261 gggtgcacct gattatccag cagaatcgct ttccctgcgg cagccgccgc atcaccagta
     4321 tcagcgaggt gtcgggtgtt gagtccaaca ccgtccagtt gggggaaatc ttccgcttcc
     4381 agcaggatgg tttcgatgca gacggcaagg tcagagggcg cttcatggca accggctata
     4441 ttccggtgtt tctggaaaag ctgcaggagc ggggcaccaa aattgatttt tcattgtttg
     4501 aataaaggtg gatcacgtga gtggtgacaa gtcgcgcagc gatgtggcat tgcctgtgaa
     4561 cgggcaaacc ggaaccttcc gccgtctgct gggctacacg ttgcagcaca agcgcgagct
     4621 gacgattgcc atcgcgctgt tgctggtggc gacactcgcc gacgtggcgg gtccgctgct
     4681 gatcaaaacc tttatcgacg attacctggt gccgggcaac tggaatttca accatatcgg
     4741 cgcgatgctg gtgatttacg tggtggcgaa tatcaccgcc gcctttctgg gttttttcca
     4801 gtccctgcgc tggaacagca ttgcccagaa agtggtgatg aaactgcgcc accagatttt
     4861 ttcccgggtg gtgtcgctgc cattgaagcg gttcgactac acgcccaccg gcagcctgat
     4921 ttcccgcatt accaatgaca cggaatcggt gaaggagctg ttcgtcggtg tgctgggtgt
     4981 gtatatccag aacagtgtgc gcgtgatcgg tattttcatc gcgatggccg tgctgaactg
     5041 gaaactgatg ctggtgtgtc tgctgttcgt gcccgccgtg attttcgtga tgttctgcta
     5101 ccgccggttg agcacgccag tgttccagcg tgcgcgggaa ctgctgtcgg acatcaatgc
     5161 ccgcatgaat gaaaccatcc agggcgtggc cgtggtgcag ctgttccggc aggaacagcg
     5221 ttttgccgac cagtaccacg cggtgacgga ggagcatttc agtacccgcc gtcgcaccat
     5281 gcagatggat gcgctgctgc tgcggccgat ggtggacttg ctgcatctgc tcaccctggg
     5341 tggtctgctg tatttttttg gccacaatgc gctgcagaat gccagcggcc tgatcgaagt
     5401 gggggtaatt tacgctttcg tcagctatct gggccgattc acagaaccgc tgattgaaat
     5461 gacccagcgg cttaacctgt tccagcaggc catcgtgtcg gcgggacggg tgttcgcgtg
     5521 gatcgatgaa gcgccggaac aggtgcagcg caaggcggcg gtcagtcccc gcgtgggcga
     5581 tatccgtttt gataatgtga gcttcagcta cgatggtatt aagcccgtcc tgagcggcat
     5641 caaattccat atcggtgcgg gccagtttct gggtattgtg ggccacaccg gcagcggcaa
     5701 gagcactatc gccagcctgc tgttgcggtt ctacgcgccg acgcaaggcg gtatcgcgct
     5761 cggcggcgaa gatctggcgg aattttccca ggatatgttg cgcaggaccg tcgccatcgt
     5821 gcagcaggac agtttcatct tcgcagcgtc ggtggcggat aacatcgata tcggccgcgg
     5881 cctgtcccgt gccgatattg aagcggctgc aaaagcggtg gggctgcatc cccacgtttt
     5941 gcagttgcag aatggttacg attccatgct ggatgaaaag ggccagtcgc tgtcggtagg
     6001 gcagcggcag ttgctttcac
//
LOCUS       DASUEG010000722         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1822258, whole genome shotgun sequence.
ACCESSION   DASUEG010000722 DASUEG010000000
VERSION     DASUEG010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822258"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VFX11_13645"
     CDS             complement(<1..424)
                     /locus_tag="VFX11_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939717.1"
                     /translation="MYRFHEQADGSAHRVAVTLCRLLREMKLQAGIDAEPASTVDRAW
                     RVATFGTAVSQLCAVSTNEFIGLDYGARLALESDVIATVDGASWTSLLDLLELLKKFH
                     AIAGITVSTRIVFEHDECVLDAALPYQNIPAPVERFAMQ"
     gene            883..2475
                     /locus_tag="VFX11_13650"
     CDS             883..2475
                     /locus_tag="VFX11_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939718.1"
                     /translation="MSISDDVLPPGKLALLPGSVRPAAILNGRDGFSPMGAAMFELTS
                     EALADQLAADPQQWVHAYSVDTGDPIDVVAARSPIERSSQFSWGPIIEVYPASRYPFG
                     ERIVIVISMPDNAAVPDDYAAPARELSEAAWQLLEHNDIAAQSVVTATEYTIASAESV
                     LGEHERGQAAITDFDYPIEIVRTLYYRKGHVAAKVKGRVLLPDFRGEDGLVNVNNDAQ
                     ANAQWIPFDLYLPRSARKASAPVMIYGHGITANRYTSIFVHRQNARHGIATLAIDQPN
                     HGSRISIDGYSVFQVLRTDRLPRALGMVMQSAFDLHGLTSMIEKQLHTVDALPKKIWG
                     RSGDGIPDIDTSRILYSGTSLGGVLGSGFAAFDDRVDGVYLQVSGTGIARIMTHSSFW
                     GLGFKNLISREFTAGETAFQAMLTQHVVDMVEGGMRAESYNRETKPLFIQYGLDDTVA
                     TNASTFSLAGIAGLQLNPPVLADVPGMPVNGVYDPLMGVLQVEPVVRKGWLHGFTAHT
                     TFIKSQAIDAYRQWLDAINARW"
     gene            complement(2568..>2735)
                     /locus_tag="VFX11_13655"
     CDS             complement(2568..>2735)
                     /locus_tag="VFX11_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX4939719.1"
                     /translation="SLAELQQFSPIITDDVFAVLTLEGSVNARNHLGGTAPAQVKAAV
                     QRAKAKLAERK"
BASE COUNT          543 a          744 c          810 g          638 t
ORIGIN      
        1 cctgcatcgc aaagcgctcg accggcgcgg gaatgttctg gtagggcagc gctgcatcca
       61 gcacgcattc gtcatgctca aatacgatcc gggtcgatac cgtaattcct gcaatggcgt
      121 ggaatttttt cagcagttcc agcagatcga gcaggctggt ccaggatgcg ccatcaacag
      181 tggcgatgac atcggattcc agtgccagcc gggcgccgta atcgagcccg atgaattcat
      241 tggtgctgac cgcgcataac tggctgactg cggtgccaaa tgtggccact ctccatgcgc
      301 ggtcaacggt gctggcgggt tcggcgtcaa tgccagcctg cagcttcatt tcgcgcagca
      361 gcctgcaaag cgtaaccgcc acgcggtgtg cgctgccatc tgcctgttcg tgaaaacggt
      421 acatagtcgg atgcttccgg tttgcctgtc aggggcttct gtccatttac cctcttatca
      481 atcttctttt aatggtttat gcggtgtctt gcaaggtggc gtctttccag ggggtgtctt
      541 gcagggcgga gatttggcat cggcgcagcc aattgaaaaa aactgacctg atgctcctgc
      601 ggcaggtgca atagcatcgg gtgctgtcct ccggcttgtc gatggatgat gcaggcatat
      661 caggggttta tgagcccgca gaaggctgaa taacaacaat aatttgcagg aatcatgata
      721 atgaatattg tcagatgcgg tgttttcgct atcggatgtg tttttgcagg tagcgtgaat
      781 gcaggtgcgg cctgtaccgg acttgaaacg catacctgcg ccttgccctt tcccagtaac
      841 gagttttccc tggtaaatgc ggccactgca accgggattg agttgtctat cagcgatgat
      901 gtgctgccac ccggcaagct tgcactgttg cccggcagtg tgcggccggc ggcaatcctg
      961 aatggccgcg atggattcag tccgatgggc gcggcgatgt ttgaattgac cagcgaggca
     1021 cttgccgacc aactggcggc agacccgcag cagtgggtgc atgcttattc cgttgatacc
     1081 ggcgatccga ttgatgtggt ggcggcgcgc agcccgattg aacgttctag ccagttttcc
     1141 tgggggccga ttatcgaagt gtaccccgca tcacgttatc cgtttggtga gcgcatcgtg
     1201 atcgtgattt cgatgcccga caacgcagcg gtcccggatg actatgccgc gcctgcccgt
     1261 gagttgagtg aagccgcgtg gcagctgctg gaacacaatg atatcgccgc gcaatcggtg
     1321 gtaacggcaa cggaatacac cattgccagt gcagagtcgg tactgggcga gcatgagcgc
     1381 ggccaggcag ccatcaccga ttttgattat ccgattgaaa tcgtccgcac cctttattac
     1441 cgcaaggggc atgttgccgc caaggtgaaa ggcagggtgt tgctgccgga tttccgcggc
     1501 gaagacgggc tggtaaacgt caacaacgat gcccaggcca atgcgcaatg gattccattt
     1561 gatctgtatc tgccgcgcag tgccaggaaa gcctctgcac ctgtcatgat ctacggccat
     1621 ggcatcaccg ccaaccggta cacctcgatt tttgtgcacc ggcaaaacgc ccggcatggc
     1681 attgcgacgc tggcgatcga tcagcccaat catggttcgc gcataagcat tgatggctac
     1741 agcgtattcc aggtgttgcg taccgaccgg cttccgcgcg cgctcggcat ggtgatgcaa
     1801 tccgcgtttg acctgcatgg cctgacgtcc atgattgaaa aacagctgca cacggttgac
     1861 gcgttgccga agaaaatctg gggccgttcc ggtgatggta ttcccgacat cgacacgtca
     1921 cggattttat attccggtac ttcgctgggt ggcgtgttgg ggagcggttt tgcagcattc
     1981 gacgaccggg tggatggcgt ctatctgcag gtgtcgggta ctgggattgc gcgcatcatg
     2041 acgcattcat cattctgggg ccttggcttc aaaaacctga tctcgcgtga atttactgcc
     2101 ggcgaaaccg cgtttcaggc catgttgacg cagcatgttg tcgacatggt cgagggaggc
     2161 atgcgggccg agtcgtacaa tcgcgagacg aaaccgttgt tcatccagta tggcctggac
     2221 gataccgttg ccaccaatgc gtcgacgttt tcactggcag gaattgccgg gttgcagttg
     2281 aatccgccgg tactggctga tgttccgggc atgcctgtga acggtgtcta tgatccgttg
     2341 atgggcgtat tgcaggtgga acccgttgtc aggaaaggct ggctgcatgg tttcaccgcg
     2401 cacaccacct ttatcaagtc tcaagcgatt gatgcgtatc gccagtggct ggatgcgatc
     2461 aatgcacgct ggtgaaactg gctggaccaa ggtgcggcgg ccagcagtgc agccgcccgc
     2521 gccttgtcgt tgtgtggaag cattctgaag gaattcagcg cttcccgtta cttccgctcc
     2581 gccagcttcg ccttcgcccg ttgcactgcc gccttcacct gtgccggcgc cgtgccaccc
     2641 agatgattgc gcgcattcac cgaaccttcc agcgtcagta ccgcaaaaac atcatcggta
     2701 atgatcggac tgaactgctg caattccgcc agcga
//
LOCUS       DASUEG010000723         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2135943, whole genome shotgun sequence.
ACCESSION   DASUEG010000723 DASUEG010000000
VERSION     DASUEG010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135943"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2824
                     /gene="tssM"
                     /locus_tag="VFX11_13660"
     CDS             <1..>2824
                     /gene="tssM"
                     /locus_tag="VFX11_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX4939720.1"
                     /translation="LNQRFRDGMAILRKAKFDSSNGKVALYQLPWYIIIGPPGAGKTT
                     ALVNSGLEFPLADSHGKGALGGIGGTRNCDWWFTNEAVLIDTAGRYTTQDSHKVVDNS
                     AWQTFLTLLKKYRPRRPVNGALIAISVQDLMTQQPAQRSHAAKQIRERIDELQKAFGI
                     QVPIYVLLTKCDLLAGFTDFFANLTQPERQQIWGVTFNAKAGKQQDAQQGLASLPAEL
                     DELVLRLNQRVLWRVHNERDPAKRALIQGFPLQVEALKPLVCDFMNEAFSANRFSDAP
                     VLRGVYLCSGTQEGTPIDRMMAAVSANFGLSREVLRNAPGGGKSFFLNRLFRTVVIPE
                     SELVGSNRQVETSLLWLRRVYFGLLALVVVGALAVWTATVKRNDDFMAQVSDLAGQFK
                     TARDGLNNQDRELPSTLPSLNPLRSAAGVYNQEEHPWLSGMGLYDASVDAAADKLYHQ
                     ALQKDYLPRFGNLIEQHLNSLNADDQALIDTLRAYLMLGDPGHFDAGTVKRWAQQQWV
                     KQYPGEASKQQELMDHLTHLLGKGFEPLALNAQVVESARMKARQIPAAQRLYSQLKAG
                     PLGQEQLDLYAQMGAKAAAQFGYSGSTPELMMLALFTKPGYDSLDFSTDSPLLKQLEA
                     DRWIYGDEQVDDFTDADKEALAAELKDLYMSDYRSQWRSFLQLLHIKPFGELQAATNG
                     LKQLADPVYSSIVAVLKVVREHTSLTPGWPQPPAIPGASAAVKDAAGKLLGKVITPTP
                     VDIEFRELNQLTQQTEQQPAGINDILAALKELHGYFNDMAVAPNPNEAIFAAAKERYS
                     GNAADPLRKIRVLASNAPTPVNLWLTESANHIWGVMLGGAKGQLNASWKKEVADKFNS
                     TLANRYPLRNGGSEASLEDFNNFFMPGGIEQAFVQANIKPFLDSSGRVKAVEGQSLGI
                     SGDALAQIRTADTIRTAFFAGGQENGT"
BASE COUNT          592 a          908 c          853 g          471 t
ORIGIN      
        1 cgctgaacca gcgtttccgc gacggcatgg cgatcctgcg caaagccaag ttcgacagca
       61 gcaacggcaa ggtcgcgctg tatcaactgc cctggtacat catcatcggc ccgcccggtg
      121 ccggcaaaac cactgcgctg gtcaattccg ggctggaatt tcccctggcg gacagtcatg
      181 gaaaaggtgc gctcggcggc atcggtggca cgcgcaactg cgactggtgg tttaccaacg
      241 aagcggtgct gatcgacacc gccggacgct acaccaccca ggacagtcac aaggtggtgg
      301 acaattccgc ctggcagacc tttctcaccc tgctgaaaaa ataccgcccg cgccggccgg
      361 tgaatggcgc gctgatcgcc atcagcgtgc aggatctgat gacccagcaa ccggcccagc
      421 gcagtcacgc cgccaagcag atccgcgagc gcatcgacga actgcagaaa gcgttcggca
      481 tccaggtgcc gatttacgtg ctgctcacca agtgcgacct gctggccggc ttcaccgact
      541 ttttcgccaa cctgacccag ccggaacgcc agcaaatctg gggcgttacc ttcaatgcga
      601 aagcaggcaa gcaacaggat gcccagcagg gtctggccag tttgcccgcc gaactggacg
      661 aactggtgct gcgcctgaac cagcgcgtat tgtggcgcgt gcacaacgaa cgcgatcccg
      721 ccaagcgggc gctgatccag ggctttccgc tgcaggtgga agcactcaag ccgctggtgt
      781 gcgacttcat gaacgaggcg ttttccgcca accgcttcag cgatgcgccg gtgctgcgcg
      841 gcgtgtatct gtgcagcggc acccaggaag gcacgccgat cgaccggatg atggcggcgg
      901 tgtcggccaa ttttggtctt agccgcgaag tactgcgcaa tgcgccgggt ggcggcaaga
      961 gtttcttcct caaccgtctg ttccgtactg tggtgattcc cgaatcggaa ctggtgggca
     1021 gcaaccggca ggtggagacc tcgctgctgt ggctgcgcag ggtttatttt ggtctgctgg
     1081 cactggtggt ggtgggtgcg ctggccgtgt ggacagccac cgtcaaacgc aacgacgatt
     1141 tcatggccca ggtgagcgat ctcgcaggtc agttcaaaac cgcccgcgac ggcctcaaca
     1201 atcaggatcg cgaactgccc agcaccctgc cctcgctcaa tccgctgcgg tcggctgccg
     1261 gtgtctataa ccaggaggaa catccctggc tgtccggcat gggcctgtat gacgccagtg
     1321 tcgatgccgc cgccgacaag ctgtatcacc aggcactgca gaaggactac ctgccccgct
     1381 ttggcaatct gatcgagcag cacctgaaca gcctcaacgc cgacgaccag gccctgatcg
     1441 ataccctgcg cgcctatctg atgctgggcg accctggcca tttcgacgcc ggcaccgtga
     1501 agcgttgggc gcagcagcaa tgggtgaagc agtaccccgg cgaggccagc aagcagcagg
     1561 aattgatgga tcacctgact catctgctgg gcaaaggttt cgaaccgctg gcgctgaatg
     1621 cccaggtggt ggaaagcgcg cgcatgaagg cgcggcagat tccggcagcg cagcgcctct
     1681 acagccagtt gaaagccggc ccgctcggcc aggagcaatt ggatctgtac gcccagatgg
     1741 gtgccaaggc cgccgcccag ttcggttaca gcggcagcac gccggagctg atgatgctgg
     1801 ccctgttcac caagcccggc tacgacagcc tggatttcag caccgattcg ccgctgctga
     1861 agcaactcga agcggaccgc tggatttacg gcgacgaaca ggtggatgac ttcaccgacg
     1921 ccgacaagga agcgctggcg gcggaactga aagacctgta catgagcgac taccgttccc
     1981 agtggcgcag ctttctgcaa ttgctgcaca tcaagccctt cggcgaactg caggccgcca
     2041 ccaacggcct gaagcagctg gcggacccgg tctattcctc catcgtggcc gtactgaaag
     2101 tggtgcgcga acacaccagc ctgacgcccg gctggccgca accaccggca attccgggcg
     2161 cctctgctgc ggtgaaagat gcggccggca aactgctggg caaggtgatc acacccacgc
     2221 cggtggacat cgaattccgc gaactgaacc agctgaccca gcagaccgag cagcagcccg
     2281 ccggtattaa cgacatcctg gcggcactga aggaactgca cggttacttc aacgacatgg
     2341 ccgtggcgcc caatcccaac gaggcgattt tcgccgccgc caaggaacgc tacagcggca
     2401 atgccgctga cccgctgcgc aagatccgcg tactggccag caacgcaccg acgccggtca
     2461 atctctggct taccgaatcc gccaaccata tctggggcgt gatgctgggc ggcgccaagg
     2521 gccagctgaa tgccagctgg aaaaaagaag tggcggacaa attcaacagc accttggcca
     2581 accgttatcc gctgcgcaac ggcggcagcg aagccagcct ggaggacttc aacaatttct
     2641 tcatgcccgg cggcatcgaa caggcattcg tgcaggccaa catcaaaccc ttcctcgaca
     2701 gcagtggccg ggtgaaagca gtggaaggcc agtcgctggg aatctccggc gacgccctgg
     2761 cccagatccg tactgccgat accatccgca ccgccttctt cgccggcggc caggagaacg
     2821 gcac
//
LOCUS       DASUEG010000724         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1724588, whole genome shotgun sequence.
ACCESSION   DASUEG010000724 DASUEG010000000
VERSION     DASUEG010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724588"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFX11_13665"
     CDS             complement(<1..606)
                     /locus_tag="VFX11_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939721.1"
                     /translation="MLQNIRKFAALHGILIVALVLSSFAGLSPVDNVQAQTAPPANFT
                     PRRMSPDLMAVPFTDKTDIKVLVQVNGPISPELHAFLTYNEKDIKLEAKFVNFPILAG
                     KVSTAGLAGLLNFPEVHYVSLRKQLKQLGHVTETTGAASARAAFGNGLTTETTLDGKG
                     IGVAVIDSGIDKGQKSFTNNGTNNVVFKKNFIATETTTNDLY"
     gene            complement(1070..3067)
                     /locus_tag="VFX11_13670"
     CDS             complement(1070..3067)
                     /locus_tag="VFX11_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4939722.1"
                     /translation="MNRKYNQMNLPGSALTLGKRQMPVAGTKKEQGVKYSSKQVVWLL
                     LVVFILSLSSLTTLAQTRPKVSADLLALIAKGGTTKAAVIVTTPSTPSSSLLTAVKGT
                     GGTVKKNYSKVTAISADLPVAQVNSIATRTDVSYVALDRVAQVTGHLEATTGATLARN
                     LGTSTTGTLNGKGIGIAILDSGIYGAHYSFLKNYDSDTSGSRVLYNIDFTGTGTTGDI
                     YGHGTHVAGMAAAGPRVAEGAYTGIAPLANIVNVRVLDANGRGSASNVIAGIDWCITN
                     KATYNIRVMNLSLGVTAVDSYKDDPLCLAVRRAVDAGIVVVVAAGNLGKDEQGNKVYG
                     GIHSPGIEPSAITVGAANTFGTDARADDVVTTYSSRGPTRGYYTDTAGVKHYDNLIKP
                     DLIAPGNRILAAQASNNNLVLATPELDANVSSNIVKDQMFLSGTSMAAPAVAGAAALL
                     LQRNPSLTPNLVKALLQYTAQPLAYANNFEQGTGLLNVEGAVRLAGLVRTDLSGRVKG
                     DPLLCTGCSAPTQTSTIAGSSFAWGGAILQNWNFLTGANLVLKYQGIYGTGIILGDGT
                     VLGDGIILGDGTLLSDGIIIGDGAVQSDGTTWSSSNQVLVARGVVLGDGIILGDGIIL
                     GDGIILGDSVLSNNAQATALANGVLKTGDNTTKMRVVVDLR"
     gene            complement(3433..>3894)
                     /locus_tag="VFX11_13675"
     CDS             complement(3433..>3894)
                     /locus_tag="VFX11_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939723.1"
                     /translation="GAVRLAKLVRTDLTNNTAVGSPLLTAAAPSPASTIAGHSFSWAQ
                     GLVMGYSTVTGSNLITKYQSVYGIGPILSDGLIFADGLILADSRVLSDGLLLANYVLT
                     SNGLILADGVPFLNTNLLAGNGLLLADGMLMSDGIMLADGVALADGIMAAD"
BASE COUNT          828 a         1108 c         1082 g          876 t
ORIGIN      
        1 atacaggtcg ttggtggtgg tttcagtagc gatgaagttt ttcttgaaga caacgttatt
       61 agtgccgtta ttggtgaagg acttttgccc cttgtcaatg ccggaatcaa tcaccgccac
      121 gccaatgcct ttgccatcca gcgtcgtttc tgtagtgagg ccattgccaa acgctgcccg
      181 cgctgacgcc gcgcccgtcg tttcagtcac atgccccagt tgcttgagct gtttgcgcag
      241 cgaaacgtaa tgcacttcgg ggaaattcag cagcccagcc aatcccgccg tactgacctt
      301 tcctgcgagg atcgggaagt tcacaaattt cgcctcaagc ttaatgtctt tttcgttgta
      361 agtgagaaag gcatggagtt cggggcttat cggcccattc acttgcacga ggaccttaat
      421 gtcagtctta tccgtgaagg gcacagccat aagatccggt gacattctgc gcggtgtgaa
      481 gttcgcaggg ggtgcagtct gtgcctgaac attgtccact ggggacagcc cggcaaagct
      541 ggagagtact aacgcaacaa taagaatacc gtgtagcgcg gcaaattttc tgatgttttg
      601 taacatgggg gtttcctctt tgagcaaagt agaccagccc aaacaaaaag gccggccttc
      661 gcatttaata agctcgaaga ccggcctttg acgggtcgtc tcttcgggga ctcccgctgc
      721 catagcttga agtcagatgt cagaaggatg agcgctgaag taaaagcttc agcgttcctc
      781 gttcctcgtt ccgcattcca gccccaggcc ggtgagtact tgcctgctcc ggctttcgca
      841 caggagcagg ctcttatcgg tgacgttgac tataaatcta aagagcgtat ttgcacgggg
      901 gcgataaact atgtggttct cggacacaag cgaaactacg cgagtctctt cacggcgctg
      961 tgacaagggc gtgataactt ttactgcggc tggaattcag gaggaattcg ccttggccag
     1021 gagtagacag gggcgtgtct cctgtaggct ttccgtacca ctacccgcct tagcgcagat
     1081 ccaccacaac cctcattttg gtggtgttgt ctcccgtttt cagcacgcca ttggccagcg
     1141 ccgtggcctg tgcgttattg cttagcacgg aatcccccag aatgatgcca tcccccagaa
     1201 tgatgccgtc gcccaaaatg atgccatcgc ccagcaccac cccgcgcgcc acgaggactt
     1261 gattgctgct cgaccacgtc gtgccgtcgc tttgcaccgc cccatcgccg atgattatcc
     1321 catcgctcag cagcgtgccg tcgcccagaa taatgccatc gcccagcacc gtaccgtcgc
     1381 ccaagataat ccccgtgcca tagatgccct gatatttcag caccagattc gcgcctgtca
     1441 gaaaattcca attttgcagg atcgccccgc cccaggcgaa cgagctaccg gcaatcgtgc
     1501 tggtctgggt gggcgcgctg caaccggtgc agagcagcgg atcacccttc acgcgtccgc
     1561 tgaggtcggt gcgcacgagg ccggccaagc gcacggctcc ttcaacgttc agcaaacccg
     1621 tgccctgctc gaagttgttg gcgtacgcca gcggctgcgc ggtgtattgc agcagcgctt
     1681 tgaccagatt cggtgtcagg ctcggattgc gttgcaacaa caacgctgcc gccccagcca
     1741 cggctggtgc cgccatcgag gtaccactca aaaacatctg gtctttaaca atattcgaac
     1801 taacgttcgc atcgagttca ggagtggcaa gtaccagatt attgttgctg gcctgcgccg
     1861 caagaattcg gttgccgggc gcgatgaggt cgggcttaat gagattgtcg taatgcttca
     1921 cgccggctgt atcggtgtaa tacccacggg tcgggccgcg cgaactgtag gtggtgacca
     1981 catcatccgc acgcgcgtcg gtgccaaagg tattggcggc gcccaccgtg atggccgaag
     2041 gctcgatgcc gggcgaatga atgccgccat acaccttatt accctgctca tctttgccca
     2101 aattgcccgc cgccacgacg acgacaatgc cggcatccac ggcgcggcgc acggccagac
     2161 agagcggatc gtctttgtaa gaatccactg cggtcacgcc cagcgaaaga ttcatgacac
     2221 gaatgttgta agtggctttg ttggtgatgc accagtcaat gcccgcgatc acattggagg
     2281 ccgagccgcg tccgttggcg tcgagcacgc gcacgttcac gatgttggcc agcggcgcga
     2341 tgccggtgta ggcgccttcg gcgacgcgcg gaccagcggc ggccattccg gcgacgtgcg
     2401 tgccgtgtcc gtaaatgtcg cctgtggtgc cggtgccggt gaaatcaatg ttgtacagca
     2461 cgcggctgcc gctcgtgtcg ctgtcgtagt tcttcaggaa cgagtagtgc gcgccataga
     2521 tgcctgaatc cagaatggcg atgccgatgc ctttgccgtt cagggttcca gtggtggagg
     2581 tgccgagatt cctggcaagc gttgcgcccg tggtcgcttc gaggtggccc gtcacttgtg
     2641 ctacgcgatc aagcgcgaca tatgagacat cggtgcgggt ggcaatacta tttacctgcg
     2701 ctacaggcaa atcggctgag atggccgtaa ccttggagta gttctttttg accgtgccgc
     2761 cagtgccctt taccgcagtc agtagggatg aggagggcgt ggagggggtg gtgacaatga
     2821 cggcagcctt cgtcgttccc cctttggcaa ttaatgccaa caggtcagcc gaaactttcg
     2881 gccttgtctg tgccaaggta gtaagagagc tcaacgacag gataaagaca accaggagca
     2941 accatacaac ttgcttggag ctatacttca cgccctgctc tttcttggtg cctgccaccg
     3001 gcatctgcct ctttcctaat gtcagggcgg agcctggcaa attcatctgg ttatattttc
     3061 gattcatgat tttccttttc gaataaagaa gcccagccca aacgaaaagg ccgatcttct
     3121 cacctgtaaa tgcgaagacc ggcctttgct ttttcggtcg tctcttcggg ggcctccgct
     3181 gccatcccac gtgtgtggat taggccggtc ggcacttacc cgctctggct ttcgcacaaa
     3241 gaaggctctt atcggtgacg ttgataatta aataaaaaga gcgtatttgc ccgggggcaa
     3301 taaatctggt tctctgacca cggagaggct accggaaatt gttcacagac tcataatatg
     3361 ccagtaataa gttctgcttg cttgggaagg gcagcccgat tccgactgcc gtcgcctggc
     3421 tttggccgga cttcaatcag ccgccataat gccatcggcc agggctaccc catcggcgag
     3481 cataatgcca tcggacatca gcattccatc cgccagcaac aatccgttgc cagccagtaa
     3541 attggtattc aggaatggca cgccatcggc cagaatcaag ccgttgctgg tcaagacgta
     3601 attggcgagg agcaagccat cactcagcac ccggctgtca gcaaggatca agccatcggc
     3661 gaaaatcagc ccatcactca ggatcgggcc aatcccataa acgctttgat atttggtgat
     3721 caggttggag ccggtgaccg tcgaatatcc catcaccagc ccctgtgccc aggagaaaga
     3781 gtgacccgca atcgtggaag cgggcgaggg agcggcggca gtcaacagtg gagatcctac
     3841 tgccgtgtta ttggtcaggt cagtgcgcac cagttttgct aatcgcacag cgcc
//
LOCUS       DASUEG010000725         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1160486, whole genome shotgun sequence.
ACCESSION   DASUEG010000725 DASUEG010000000
VERSION     DASUEG010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160486"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..689
                     /gene="rpsD"
                     /locus_tag="VFX11_13680"
     CDS             69..689
                     /gene="rpsD"
                     /locus_tag="VFX11_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX4939724.1"
                     /translation="MARYLGPKCKLSRREGTDLGLKSGVRAHENKCRSEKAPGMHGER
                     RGRLSDYGTQLREKQKVRRIYGILERQFRNYYTEAARQKGATGENLLQLLESRLDNVV
                     YRMGFGVTRAEARQLVAHKAILVNGKTVNVASFQVSAGDVVAVREKAKNQLRVTAALE
                     LAEQRGFASWIEVDTKKKEGVFKAVPERADLPSDLNENLIVELYSK"
     gene            715..1719
                     /gene="rpoA"
                     /locus_tag="VFX11_13685"
     CDS             715..1719
                     /gene="rpoA"
                     /locus_tag="VFX11_13685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX4939725.1"
                     /translation="MQRSFSDFLTPRTIGVQEISSSHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGAAVTEVEIDGVVHEYSTLEGVQEDVIDILLNLKNLAVRLNEKSEAYLVV
                     SKEGPGPVTAGDIQLDHDVEIINKDLVIANLSAGAKLNMKLRIEKGRGYQPADARGGE
                     EETRGVGRLQLDATYSPIRRVAYVVESARVEQRTDLDKLVIDLETNGTIAAEDAIRRA
                     ATILQQQLAVFVDLEKEAEPQKIEERDEIDPILLRPVDDLELTVRSANCLKAENVYYI
                     GDLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLSLGMRLENWPPGSLRDDDRVLKKR
                     "
     gene            1771..2163
                     /gene="rplQ"
                     /locus_tag="VFX11_13690"
     CDS             1771..2163
                     /gene="rplQ"
                     /locus_tag="VFX11_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX4939726.1"
                     /translation="MRHRKSGRKFSRTSSHRHAMFRNMAVSLVEHELIKTTLPKAKEL
                     RRHAEPLITLAKIDSVANRRLAFNRTRSKSAVGKLFTDLGPRYKERPGGYIRILKCGF
                     RPGDKAPMAYVELVDRVVKGPAVEVDDE"
     gene            complement(2394..>3920)
                     /gene="uvrA"
                     /locus_tag="VFX11_13695"
     CDS             complement(2394..>3920)
                     /gene="uvrA"
                     /locus_tag="VFX11_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4939727.1"
                     /translation="VNDSLTYFKALKLDGKKGEIAEKILKEIRERLQFLVNVGLDYLS
                     LNRRAETLSGGEAQRIRLASQIGAGLVGVMYILDEPSIGLHQRDNERLLSTLTRLRDM
                     GNTVIVVEHDEDAIRAADFVVDIGPGAGVHGGQILRAGSIEQIMACKQSLTGQYLKGT
                     LRIEVPAQRTKANPKKQLVIKGAHGNNLKNVDLHLPVGLMTCVTGVSGSGKSTLINGT
                     LFPLASTVLNGASTLKPEPYGEIQGLDHFDKVVDIDQSPIGRTPRSNPATYTGLFTPI
                     RDLFAGTQEARSRGYGPGRFSFNVKGGRCEACQGDGVIKVEMHFLPDIYVPCDVCKGK
                     RYNRETLEIKFKGKSIQEVLEMTVEDAHVFFENVPALARKLQTLMDVGLSYIRLGQSA
                     TTLSGGEAQRVKLSKELSKRDTGQTLYILDEPTTGLHFHDIQQLLHVLHRLRDKGNTV
                     VIIEHNLDVIKTADWIVDLGPEGGNGGGQIIAEGTPEAVAKNKQSHTGRFLKSFLKSA
                     "
BASE COUNT          817 a         1028 c         1176 g          899 t
ORIGIN      
        1 gtgacgccga ttccccacaa cggttgtcgc ccgcctaaga aacgtcgtgt ttaacgggag
       61 tcctattaat ggctaggtat ttgggcccta agtgcaagct gtctcgtcgt gaaggtaccg
      121 atctcggtct gaagagcggt gttcgtgccc acgaaaacaa gtgtcgctct gaaaaagctc
      181 ccggcatgca cggagagcgt cgtggccggc tgtctgatta tggcacccag ttgcgtgaga
      241 agcagaaggt tcgtcgaatc tacggaatcc ttgagcgcca gttccgcaac tactacacag
      301 aagccgcccg gcagaaaggc gcaacgggtg aaaacctgct gcaactgctg gaaagtcgcc
      361 tcgataacgt cgtttaccgc atgggtttcg gcgttacccg cgccgaagct agacagctgg
      421 tggcacacaa ggctattttg gttaatggaa aaactgttaa cgtcgcgtca ttccaggtct
      481 ctgcaggaga tgtggttgct gtacgcgaga aggccaagaa ccagttgcgc gtaactgcgg
      541 cgctggaact ggcagaacag cgtggttttg cctcctggat cgaagtagat accaagaaga
      601 aagaaggtgt ttttaaggct gtaccggagc gcgcagatct cccgtccgat ctgaatgaaa
      661 acctaatcgt cgagctttat tccaagtaag caataggttg aggtggcacc gtttatgcaa
      721 cgctctttta gcgacttttt gacccccaga accattggcg tacaagaaat cagcagctcc
      781 catgccaaag ttacgctcga gcctctcgag cgtggtttcg gtcataccct gggcaatgcc
      841 ctccgtcgca ttcttctgtc cagcatgcct ggtgcggctg ttactgaagt ggaaattgat
      901 ggcgttgttc atgagtacag caccctcgaa ggcgttcagg aagatgttat cgacattttg
      961 ttgaacctga agaatcttgc cgtacgtctg aacgaaaaga gcgaggctta cctggttgta
     1021 tccaaggaag gccctggtcc ggtcactgct ggcgacattc agctggatca tgatgtcgag
     1081 atcattaaca aggatctggt gattgcgaat ctgagcgccg gcgcgaagct gaacatgaag
     1141 cttcggatcg agaaggggcg tggctatcag cctgctgatg cccgcggcgg cgaagaggaa
     1201 acccgcggtg ttggccgtct gcagctggac gccacctact cgcctatccg tcgtgtagct
     1261 tatgtcgttg agagtgcccg tgttgaacag cgcactgacc tcgacaagct ggtgattgat
     1321 ctggaaacca acggcaccat cgcggcagaa gacgctattc gccgtgcagc caccattttg
     1381 cagcagcaat tggcggtgtt tgtggatctg gagaaggaag ccgagcccca gaaaatcgag
     1441 gagcgtgatg agatcgatcc gatcctgctg cgtcctgtag atgatctgga actgactgtc
     1501 cgttctgcca actgcttgaa agccgaaaac gtgtattaca tcggcgatct ggttcagcgt
     1561 accgaggtcg agctgttgaa gacgccgaac ctgggcaaga agtcgctgac cgaaatcaag
     1621 gacgtgctgg catcgaaagg cttgtcactg ggtatgcgct tggaaaactg gccgcccgga
     1681 agtctgcgcg atgatgatcg cgtactgaag aagcgttaat ttcatctttc tgtcccaagg
     1741 ggcagacgtt agttgtaaag gaaaagagca atgcgtcacc gtaaaagtgg tcgtaaattc
     1801 agtcgtacaa gcagccatcg tcatgcgatg ttccgtaaca tggccgtgtc actggttgag
     1861 catgagttga tcaaaactac cctgccgaaa gcgaaggaac tgcgtcgtca cgccgagccg
     1921 ctgatcacgc tggcgaagat tgacagcgtt gcgaatcgtc gcctggcgtt caaccggact
     1981 cgcagcaaat ccgcggttgg caagctgttc actgacctgg gcccccgtta taaggagcgt
     2041 cccggaggct atattcgcat cctgaagtgc ggattccgtc ccggtgacaa ggcgccgatg
     2101 gcgtacgtcg agctggtgga ccgcgtggta aaaggtcctg cagtggaagt tgatgacgag
     2161 taaaattgcg tcgctcgatt tgaaaaagcc ggcttgtccg gcttttttta tgccttggat
     2221 ttgcgggtat gtggggagac ttgataagcg caggtttgat gagcgattgc acacgagagc
     2281 atacgttctc aaccatcaag cctgcctttg ttgccaatgg ccccgaatgt cttaacggca
     2341 ggtcccatga atggactgcc agtaaaggga ggcctgactt tttgtataaa ttctcaggct
     2401 gatttcagaa agcttttcag aaaccggccg gtatgggact gcttgttttt ggctacggct
     2461 tctggcgttc cttctgcgat gatctgtcca ccgccattgc caccctccgg ccccaggtcc
     2521 acgatccagt ctgcggtttt gatcacgtcc aggttgtgtt cgatgatcac cacagtatta
     2581 cctttgtcgc gcagccggtg gagcacgtgc agcaattgct ggatgtcgtg gaagtgaagg
     2641 ccggtagtag gttcgtccag aatataaagg gtctggccgg tgtcacgttt cgataactcc
     2701 ttggacagct tcacccgctg cgcctcgcca cccgagagcg tggtggcgga ttgccccagt
     2761 cttatatagg acagcccgac gtccatcagt gtctgcagct tgcgcgccag tgccgggaca
     2821 ttctcgaaga agacgtgggc gtcttccact gtcatttcca gcacttcctg aatgctcttg
     2881 cccttgaatt tgatctccag tgtttcgcgg ttgtagcgct tgcccttgca gacgtcgcag
     2941 ggtacgtaga tatccggcag gaagtgcatt tccaccttga tcacgccgtc gccctggcag
     3001 gcttcgcagc ggccgccctt gacgttgaag ctgaaacggc ctgggccata gccccgggag
     3061 cgggcttcct gggtgccggc gaacaggtcg cggattggtg tgaacagccc ggtgtaggtg
     3121 gcaggattgg agcgtggcgt gcggccgatg gggctctggt cgatatcgac gaccttgtcg
     3181 aaatggtcga ggccctggat ttcgccgtag ggctctggtt tcagcgtgct ggcaccattg
     3241 agcacggtgg aggcgagcgg gaacagggtg ccattgatga gggtagattt acccgagccg
     3301 gacacgccgg tgacgcaggt catcaggccc accgggaggt ggaggtcgac gttcttcagg
     3361 ttgttgccgt gtgcaccctt gatcaccagt tgtttcttgg gatttgcctt ggtgcgttgc
     3421 gccggcactt cgatgcgcag tgtgcctttc aggtactggc cggtgaggga ctgtttgcag
     3481 gccatgatct gttcgatgct gccggcgcgc aggatctggc cgccgtgaac gccagcgccg
     3541 ggaccgatat ccaccacgaa atcggcggcg cggatggcgt cctcatcgtg ctccaccacg
     3601 atgaccgtgt tacccatgtc gcgcaggcgg gtgagggtgc tcagcaggcg ttcattgtcg
     3661 cgctggtgca ggccaatgga cggttcgtcc agaatgtaca tgacgcccac cagccccgcg
     3721 ccgatctggc tggcgaggcg gatgcgctgt gcttcgccgc cggacagggt ttcggcgcga
     3781 cgattcagcg acagatagtc cagacccaca ttcaccagga actgcagccg ctcgcggatt
     3841 tccttgagaa tcttctccgc gatctcgcct ttcttgccat ccagtttcag cgccttgaaa
     3901 taggtcagcg agtcgttgac
//
LOCUS       DASUEG010000726         7084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1996952, whole genome shotgun sequence.
ACCESSION   DASUEG010000726 DASUEG010000000
VERSION     DASUEG010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996952"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="VFX11_13700"
     CDS             <1..2265
                     /locus_tag="VFX11_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4939728.1"
                     /translation="EKIALRFLSFNNTDRELSYRELMVQVSSAAAHIQCHCAVGDRAL
                     ILLPAGVDFVSWFVGCFYAGAVATPTAVPVQRREWERLGNILRDAKPSVLITDGATLE
                     KLQGAGVLAQVHQAGVKIIDVGAVSLAPKAAPVEVTPDMVAFLQYTSGSTGNPKGVMV
                     SHGNLMHNEATIQSGLRSTQDSVMVSWLPLFHDMGLIGQMLHSLYMGATLCLMSPESF
                     LRDPLRWLESIHRFHGTIAGGPNFAYELCIKKANAAPDRVAALDLSCWHTAFNGAEPV
                     RVETLTRFAEKFAGSGFRQESLYPCYGLAECTLMISGATRQEVPTVAQLDDDRLRERR
                     IARSSAATSKRMVASGKVLGGHQLRIVDPDTLAPCGPDQVGEIWVQGGSVGQGYWNNP
                     EASAKDFQAFTSTGEGPFLRTGDLGFLLDDYLYISSRCKDLIIINGKNFYPQDIERSA
                     EAGHTALRPGCSAAFSVENNGTEQLVVLCEIDPAKGGEADFPAIMAAVRAKVFEQQSA
                     SVARIALYAPNTLFKTSSGKIQRRSCNAAFTAGKLSALCQWDAPQASAAPAAAVVPEA
                     GSSDILLRSLVTHLGKQRELLAAFASHMSESMRQLDLLMSPHQLEAPSAAAAIDVAKS
                     GPARVNTAPVAVSTPAGPSRAAIRLWLTRYLTSQLGLSTLVVNDHTAFADYGLDSAQA
                     LEFCAAVSDWIGFDVPATQLWDCPNLGEFTQTLHERVLLGAQNEPAASADNAWVGAMQ
                     DKDLKSLIAADLMR"
     gene            2300..6904
                     /locus_tag="VFX11_13705"
     CDS             2300..6904
                     /locus_tag="VFX11_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939729.1"
                     /translation="MGNLSSEALLKKLYVELLDTRKELNAMKANHGLEMAVVGAACRF
                     PGGVDSLESFWDLLCSGRDPIGPVPASRWNHADYFDASPDAPGKAYARESGFVGDVSQ
                     FDAGFFDISPREAAEMDPQQRMLLELSWETFHHAGQDLLAMKGSHTGVFVGMSTDDYA
                     GLTVQSGDHSQIDAYNSLGTARSVAAGRVAYTWGFHGPVIQLDTACSSSLAALHLACQ
                     SIASGDCEQALVAGINLMLAPELHIACSRLRALSPTGRCKVFDDSADGYVRSEGAGCV
                     LVKPLARALEDGDRVLGVIKSCAINHDGKSNGLTAPNGRAQVQLIRKALKRANLDASR
                     IGYIETHGTGTRLGDPIEVHALGDALQRKKRGATAQSPLLLGSVKSNIGHLEAAAGMA
                     SLLKTLLIQQNGFIPANLHFSEPNRQIRWDQLDVQVVNSAQPWPQQPQSDLIGISAFG
                     LSGTNVHVIVGPAPDAELQPVSAPQRDAHVFTLSARNADALQGQLQQCRTALEKLDVA
                     RFADWCTAHTLHSTHFPLRKALVGGNSEELRLALDIALQAPAMPVHARVDQICFVFTG
                     QGAQYPGMGRELAAQIPAFRQHMDRLAPLFQQILGLPLDELWWGEFSPRLNQTQFTQP
                     ALFAFEYCLAQTLMEWGIRPDMCLGHSVGEYVAACVAGVLSLEDALRCIALRARCMAE
                     CEAPGVMVAVLAQAERVQDAIQKTASATADKVAIAAYNSPNSTVMSGDRAAVLAVMDH
                     LTEAGVRCVQLDVSHAFHSPLMQPAADALVAQLPTLAVQPGALPVISNVSAGLLDPAQ
                     LTPAYLARHIVEPVRFTDSIRFAVEQGANLFIEIGPKSTLTQLLGQIVPDAGIQAFAA
                     IAQARQPIHSLLNCLGKAYEAGLNPAWQAALGGGRLQKVERLTYPFSRQSLWTRSTKR
                     QLVAAPGSSLYRLAWTAVATETTSVEAGDWLLVRADSCESSWLQENLANAGARVQCLT
                     LSDANVAEWAAQVKTALQEKNWRGLILQTDAQMSLDAVSYARVLNDIERCRLLLEQWE
                     MQCAGENSRTQRGLWLVTPAMHAEISALALAPLQGFVKGASLEKRAAWGGYLELKDDT
                     AASYARAVKALLGDARDLLQVENGVLLQPRLQVVTPPPATELRFSGDDAVLIAGGAGG
                     IGLNLCGWCVERGLRRLLVVGRRSAQELGEAAEGLRKLEERGVTVWYRQLDIADSAAT
                     AATVAEFESAVGPLTGFIHAAGVASLRNLDQLDETQLRATLDSKLWGAWNLHQLTLDR
                     TVKLFALFSSISSLWGGLQLAHYAAANACLDALTELRLQQGLPVHCIQWGPWKEAGMA
                     RDQVSHDMSRMGVRAFDNAAAQKAFFRAVDSAAGIYCAVDADWNALARTFAANPRARL
                     FDDLVVQETSKSAQPSTLPSTQNRAAVDFSNLDATACEARINDIVMTTLTRKLQLERS
                     RIRPETPFMELGLDSILAVEIGRALEAALGMTLPATLLFDAPTAQMLVAALCKRVQPG
                     VRKIATARASNPQGQDNSLTGLIQMIRQLDDSELDRLLNA"
BASE COUNT         1376 a         1988 c         2288 g         1432 t
ORIGIN      
        1 gaaaaaatcg cgctgcgttt tctttcattc aacaatacgg atcgcgagct gagttatcgc
       61 gagctgatgg tgcaggtttc ctctgcggct gcacacattc agtgccattg tgcggtgggt
      121 gatcgcgcgt tgatcctgct gccagccggt gtcgatttcg tgagctggtt cgttggttgt
      181 ttctatgccg gcgcagtagc gaccccgaca gccgtgccgg tgcaacggcg tgaatgggaa
      241 cgtctgggca acattctgcg agacgccaag ccgtcagttc tgattactga cggggcgacc
      301 ctggagaagc tgcaaggagc aggcgtactg gcgcaggtac atcaggccgg cgtgaagatt
      361 atcgacgtgg gtgctgtgtc gctcgcgcca aaagctgcgc cggtggaagt gactccggac
      421 atggtggcat tcctgcagta cacctccggt tccaccggca atccgaaagg cgtgatggtg
      481 tcgcacggca atctgatgca caacgaagcc accatccaga gtggccttcg cagcacacag
      541 gacagtgtga tggtgtcatg gctgcccctg tttcatgaca tgggactgat cggccagatg
      601 ctgcattctc tgtatatggg cgcgaccctg tgcctgatgt cgccggagag ctttttgcgc
      661 gatccactgc gctggctgga gagcattcat cgctttcacg gcaccatcgc cggtggaccg
      721 aatttcgcct acgaactgtg tatcaaaaaa gccaacgccg ctccggatcg ggttgcagcg
      781 ctggatttgt cctgttggca cacggccttc aatggtgctg aaccggtgcg cgtggaaacg
      841 ctgacccggt ttgcggaaaa attcgcaggt agcggtttcc gtcaggaaag tctgtacccc
      901 tgctatggtc tggcagagtg cacgctgatg atctccggtg caacccgcca ggaggttcca
      961 acggttgcgc agctggatga tgatcgcctg cgcgagcgca ggattgcgcg ttccagtgca
     1021 gcgacttcca agcggatggt ggccagcggc aaggtgttgg gtgggcatca gctgcgaatc
     1081 gttgacccgg atacactggc tccgtgcggt cccgaccagg taggagaaat ctgggtacag
     1141 ggtggcagcg tggggcaggg ttactggaac aatccggagg cgtccgccaa ggattttcag
     1201 gcattcacca gtaccggcga gggtccgttt ttgcgcaccg gtgatctggg ttttctgctg
     1261 gatgattacc tttatatctc atcccgctgc aaggatctga tcatcatcaa cggcaagaac
     1321 ttctatccgc aggatatcga acgcagtgca gaagcaggtc acactgcact tcgccccggt
     1381 tgcagtgcag cgttcagtgt tgagaacaat ggcaccgagc agctggtggt gttgtgtgaa
     1441 atcgatccag ccaagggcgg cgaagcggat tttcccgcga tcatggcggc agtgcgggcg
     1501 aaagtgttcg aacagcagag cgccagcgtg gcgcgaattg cactgtatgc gcccaacacg
     1561 ctgttcaaaa catccagcgg aaaaatccag cgccgttcct gcaatgctgc cttcacggct
     1621 ggcaagctga gcgcgttgtg ccagtgggat gcgccgcagg cgagtgctgc acctgccgct
     1681 gccgtcgtac ctgaagcggg ttcgtccgat attctgctgc gcagcctggt gacccatctg
     1741 ggcaaacagc gggaattgct ggccgcgttt gcatcgcata tgtcggaaag catgcggcaa
     1801 ctggacctgc tgatgtcgcc gcatcaattg gaagcccctt ctgccgcagc ggcaatcgat
     1861 gtcgcgaaat ccggtcccgc gcgtgtcaat accgcgccag tggcggtgag tacgccggcg
     1921 ggccccagcc gtgccgccat ccggctctgg ctgacccgct atctgacgtc gcagctgggc
     1981 ctttccacac tggtggtgaa tgatcacacg gcatttgccg actacggact ggattcggcg
     2041 caggcgctgg agttttgcgc tgctgtatct gactggatcg gtttcgatgt accggccacg
     2101 cagttgtggg attgtccgaa cctgggcgaa ttcacccaaa cactacacga aagggtgttg
     2161 ctgggtgcgc aaaacgagcc agctgcgtct gccgacaatg cctgggtggg tgcgatgcag
     2221 gacaaggatc tcaaatcgct catcgctgct gatctgatgc gctgattccc gttcattggt
     2281 aatgaaggac atggcggtca tgggaaactt gagctctgaa gccctgctaa aaaaactgta
     2341 tgtagaactg ctggatacgc gcaaggaact caacgcgatg aaagcaaacc acggcctgga
     2401 aatggccgtg gtgggtgccg cctgccgctt tcccggtggt gtcgattcgc tggaatcgtt
     2461 ctgggatttg ctgtgcagcg gacgcgatcc tatcggcccg gtgccggctt cgcgctggaa
     2521 tcacgccgac tatttcgacg cgtccccgga tgcgccaggc aaggcctatg cgcgtgaaag
     2581 cggattcgtt ggcgatgtat cgcaattcga tgcgggattc tttgacatat ctccgcggga
     2641 agcagccgag atggatccgc aacagcgcat gttgctggaa ctgagttggg aaacctttca
     2701 ccacgccggc caggatctgc tggcgatgaa aggctcacac accggtgttt tcgttggcat
     2761 gagcaccgat gattacgccg gcctgacggt gcagagtggc gatcacagcc agatcgacgc
     2821 gtacaacagc ctgggtaccg cgcgcagtgt ggcggcgggt cgcgtggcgt atacctgggg
     2881 ttttcacggg ccggttattc aactggacac ggcctgctcg tcgtcgctgg cggcgctgca
     2941 tctggcctgt cagagcattg ccagcggtga ttgcgagcag gcgctggtgg caggtatcaa
     3001 tctgatgctg gcgccggaat tacatattgc ctgctcccgc ttgcgggcgt tgtcgcccac
     3061 cggccgctgc aaggtgttcg acgacagcgc cgacggctac gtgcgcagtg aaggtgcggg
     3121 ctgtgtgctg gtcaaaccgc tggcacgcgc actggaggat ggggatcgcg tgctgggtgt
     3181 catcaagagt tgtgcgatca atcatgacgg caagagtaac ggtctgacgg cgcccaatgg
     3241 tcgtgcgcag gtgcagttga ttcgcaaggc gctgaagcgc gccaatctcg acgcttcccg
     3301 cattggctat atcgaaaccc atggtaccgg cacacggctg ggtgatccga tagaagtaca
     3361 tgcgctgggt gatgcgctgc agcgcaaaaa gcgcggagcc actgcgcagt cgccgctgtt
     3421 gctgggctcg gtgaaaagca atatcggtca tctggaagcg gcggcgggaa tggcgtcgct
     3481 gctgaaaaca ctgctgattc agcagaatgg atttattccc gccaacctgc atttcagtga
     3541 acccaatcgc cagattcgct gggatcaact ggatgtgcag gtggtgaaca gcgcgcagcc
     3601 atggccgcag cagccccagt cggatctgat tggcatcagt gcgttcggcc tgagcggcac
     3661 caatgtgcat gtgattgtgg gcccggcgcc tgacgctgaa ctgcagcctg tctctgcacc
     3721 gcaacgtgat gcacacgtat tcaccctgtc ggcgcgcaac gctgatgcat tgcaagggca
     3781 attgcagcag tgccgcacag ccttggaaaa gctggatgta gcgcgattcg ccgactggtg
     3841 taccgcgcac acgctgcaca gcacacattt cccgctgcgc aaggctttgg tgggcggcaa
     3901 ttcggaagaa ctgcggctgg cgctggatat cgctctgcag gcacctgcaa tgccagtgca
     3961 cgcgcgggtg gaccagatct gcttcgtgtt taccgggcag ggcgcgcaat acccgggcat
     4021 gggccgcgaa ttggcggcac agataccggc attccggcag catatggatc gcctggcgcc
     4081 gttgttccag cagattctgg gcttgccact ggatgaactc tggtggggcg aattttcccc
     4141 gcgcctgaat cagacgcagt ttacccagcc ggccctgttc gcgtttgaat actgtctggc
     4201 gcagacgttg atggagtggg gcatccgccc ggatatgtgc ctgggccaca gtgtgggcga
     4261 atatgtggca gcctgcgttg ccggcgtgct gtcgctggaa gatgcgctgc gttgtattgc
     4321 gttgcgggcc cgttgcatgg cggagtgcga ggcacctggc gtgatggtgg ccgtgctggc
     4381 gcaagcggag cgggtgcagg atgcaattca gaaaaccgcc agtgccacag cggataaagt
     4441 ggccatcgct gcctacaaca gtcctaacag cacggtgatg tccggtgatc gcgcggctgt
     4501 gctggcggtg atggatcacc tgacggaagc cggcgtgcgc tgtgtgcagc tggatgtgtc
     4561 ccacgcgttt cattcaccgc tgatgcaacc ggccgccgat gcgctagttg cacaattgcc
     4621 aacgctggcg gtgcagccgg gcgcgttgcc ggtgatttcc aatgtcagcg cggggctgct
     4681 cgatccggcg caactgacgc cggcctatct ggcccgtcat attgtcgagc cggtgcggtt
     4741 caccgacagc attcgctttg ccgtggagca gggcgccaat ctgttcatcg agatcggacc
     4801 gaaatccacg ctcacccaat tgctggggca gatcgtgccc gatgccggta ttcaggcttt
     4861 tgcagcgatc gcccaggcac gccagccgat tcattccctg ttgaattgtc tgggcaaggc
     4921 gtacgaagcc ggtctgaatc cggcgtggca ggcagcgctg ggcggtggac gtctgcagaa
     4981 ggttgaacga ctgacctatc ccttcagccg tcagtcgttg tggacacgca gcaccaaacg
     5041 tcagctggtg gcggcgcccg gttcttcgct gtatcggctg gcatggactg cagtggcaac
     5101 cgaaacaaca agcgtggaag caggtgattg gctgctggtg cgggcagatt cgtgtgagtc
     5161 gtcctggctg caggaaaatc tggccaatgc gggtgcgcga gtgcagtgcc tgacgctgtc
     5221 tgatgccaat gttgcagagt gggcggcgca ggtgaaaacc gcattacagg aaaaaaactg
     5281 gcgcggcctg atcctgcaga ctgacgcgca gatgtcgctg gatgcagtca gctacgcccg
     5341 cgtcctgaac gacatcgaac gctgccgcct gttgttggaa cagtgggaaa tgcagtgcgc
     5401 gggtgaaaat tcccgtaccc aacggggcct gtggctggtc accccggcca tgcacgcaga
     5461 aatatcggca ctggcactgg caccgctgca gggcttcgtc aaaggcgcca gtctggaaaa
     5521 acgcgcagcc tggggcgggt atctggaact gaaggatgac acggctgcga gctacgcacg
     5581 ggcggtgaag gctctgctgg gcgacgctcg cgatctgctg caggtggaaa atggcgtatt
     5641 gctgcaacca cgattgcagg tcgtcacacc gccaccggca acggaattgc gtttcagcgg
     5701 agatgatgcc gtgctgatcg ccggcggtgc cggtggcatc ggattgaatc tgtgcggctg
     5761 gtgtgttgaa cgtggtctgc gccgcctgtt ggtggttgga cgccgcagcg cgcaggaact
     5821 gggtgaagca gctgaagggt tgcgcaaact ggaagagcgg ggtgtaaccg tttggtatcg
     5881 ccagctggat attgccgaca gcgcggccac cgctgcaacc gttgcggaat tcgaatctgc
     5941 tgttggcccg ctcaccggtt tcattcatgc ggccggtgtg gcgtcgctgc gcaatctgga
     6001 ccaactggat gaaacgcagt tgcgcgcaac actggacagc aaactgtggg gcgcctggaa
     6061 cctgcaccag ctgacactgg atcgtactgt caaactgttc gcgctgttct cgtcgatttc
     6121 ttcgctctgg ggcggattgc aactggctca ctacgcagcg gccaatgcct gtctggacgc
     6181 actgactgaa ctgcgcctgc aacagggcct gccagtgcat tgcatccagt ggggcccctg
     6241 gaaggaagcg ggcatggcgc gggatcaggt atctcacgac atgagccgga tgggtgtgcg
     6301 ggccttcgac aacgccgctg cgcagaaggc atttttccgc gcggtggaca gcgcagcggg
     6361 catttactgc gccgtggatg ccgactggaa cgcactggca cgcacttttg ctgccaaccc
     6421 gcgggcgcgt ctgttcgatg atctggtggt gcaggagacc agcaaatccg cgcagccatc
     6481 aacgctgcct tcaacgcaga accgtgccgc agtggatttc agcaacctgg acgccaccgc
     6541 ctgcgaagcg cgcatcaatg acatcgtgat gacgacgctg acccggaaat tgcaactgga
     6601 gcgcagccgc atccgtccgg aaacaccgtt catggaactg ggcctggatt ccattctggc
     6661 ggtggaaatc ggccgcgcgc tggaagccgc gctgggcatg acgttacccg ccacgctgct
     6721 gttcgacgcg cccaccgcac agatgctggt tgcggcactg tgcaagcggg tgcagccggg
     6781 tgtgcgcaaa atcgcaacgg cccgcgccag taatccgcaa gggcaggaca actctctcac
     6841 cggactcatc cagatgatcc gccagttgga cgacagcgaa ctcgaccgtt tgctgaacgc
     6901 atgacaggaa gcaggtagca gcatgaacat caaacaactg gtcaccgaac tgaccccttt
     6961 atcgcacaag caactgatgg cgctggccgt caagcagaac gagcggctgc aacagcaaac
     7021 ggcgccggtg actgcgccgg tgcgggatga tatcgccatc atcggcttgc actgcgttac
     7081 tcca
//
LOCUS       DASUEG010000727         4738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_739937, whole genome shotgun sequence.
ACCESSION   DASUEG010000727 DASUEG010000000
VERSION     DASUEG010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739937"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VFX11_13710"
     CDS             <1..1817
                     /locus_tag="VFX11_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939730.1"
                     /translation="EECVKRVESFKQRRRAGKQLEQRLADVECAALRQTPAAVDGAAP
                     GGTSSTVIAPLVTPDLPNAAAAARAVPRKISPVPRPQVSQFKDPVPVPDRWRIVDSLG
                     YQENLLDPYNRNILKGDKPLHDDWFFAATLISDTVLEPRRSVTPVGASSSGSAGATDI
                     FGSSDQMVFSQTILTELVYYKGDTTFRPPDYEFRFTPAINYNRVELDEIQGINADPAE
                     GDTRTDDHIGIQAAFADVHLRNVGTRYDFDSIRVGIQPFTADFRGFLFNDSPFGVRLF
                     GNRENNVFQYNLAWFRRMEKDTNSGLNDVSEDLRDDDIYVANLYWQDLGVLGFTSQFT
                     VLYNRNRETDIQYDNNDFIVRPSSFGEERPKEYDVTYLGYSGDGHIGWLNLSTSLYYA
                     VGTEDPAPFYGDETDISAFFAAAEAGIDQDWVRWRVSALYASGDEDPYDDVSQGFDAI
                     FENPLFAGADTSYWIRQNVPLVGGGRVALSGRNGVLNSLRSSKEQGQSNFTNPGTQLL
                     GVGADFDVLPQLRLSFNVNQLWFDDTAVLEVARNQGGIATDIGVDASVAAIWRPWMHQ
                     NVVCRLSYAQLIPGEGYQDLYLDDASPYSLLANLILTY"
     gene            1963..>4738
                     /locus_tag="VFX11_13715"
     CDS             1963..>4738
                     /locus_tag="VFX11_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939731.1"
                     /translation="MLLACAANVQASSSLGKPREIHYPVYAPSPARQASAEMDAKSAG
                     CLSCHTATDQPSMHASEGVKLGCVDCHGGNAAVNRSQNDQPGSDGYNKTLNDAHVQPL
                     YPHAWNFPNSANPERTYTLLNQESPEFIRFINPSDYRVVEEACGACHMPVIEATRRSL
                     MSTGAMLWGGAAYNNGILPFKNYILGEAYTRDGEPASITNPVEPTPEMKAMGVLPALA
                     PMPAWETVKPGDIFRVFERGGRNIQSLFPETALPNIAGNIQRLEEPGRPDIKQSNRGP
                     GTGQRISVPLINIHKTRLNDPFMWFMGTNDQPGDYRHSGCAGCHVVYANDRDPRHSGP
                     YAKFGHTGTTHTADPTIEKDETGHPLQHSFTRSIPSSQCMICHMHQPNMFMNTFLGYT
                     MWDYESDAPFMWPEKQKYPTDEEQRKILDRNPEGAAVRGKWGDLEFLKNVSSLNPQLK
                     DTQFADYHGHGWNFRAIFKRDRKGHLLDEKGNIVSNDDPQKFKKAVHMSSVHVDKGMH
                     CVDCHFGQDAHGNGHIYGEVAAAVEVDCVDCHGTAQAYPSLITSGPAALDGGQDLSLI
                     RNPDGQKRFEWVNGKLIQRSVVTPGLEWEMSLVKDSVTPGNAHYNAKAARAKLVSNDT
                     AQQTWNGKTAPNDLAHSNDKLECYACHTSWTTSCGGCHLPIEANWKTERHHYEGGETR
                     NYATYNPQVARDEIFILGLREPSSGGRVAPVRSTSALVLSSTNSNREKIYIQQPPIAA
                     SGYSSQAMNPHYPHTERKTETKTCSDCHVAEANDNNAIMAQTLGHGTQFINLVGYNAW
                     LGTENSVTAVQVTEWEEPQAVIGSYLHRYAYPDFFQQHQDRNKLLPEAHSHSSGSANC
                     VQLRGEYLYAAEGEKGFRIYDVASIANKGVSQRIITAPFSALGHDAHLETRDASCVVL
                     ATTQPVQPSRN"
BASE COUNT         1030 a         1403 c         1392 g          913 t
ORIGIN      
        1 atgaagagtg cgtcaagcgc gtggaaagtt tcaagcagcg ccggcgtgcc gggaaacaac
       61 ttgagcagcg actggcggat gtggaatgtg ctgcgttgcg gcaaacgccg gctgcggttg
      121 atggtgctgc gccgggcggc acatcgtcca ccgtgattgc accattggtc acaccggacc
      181 tgcccaacgc ggctgcggcc gctcgcgccg tgccacgcaa aatttcgccg gtgccgcgcc
      241 cgcaagtgtc gcaattcaag gacccggttc cagtgccgga ccgctggcgc atcgtcgatt
      301 ctctcggcta tcaggaaaat ctgctggacc cctacaaccg caatatcctc aagggcgaca
      361 aaccgctgca cgacgactgg ttttttgccg ccaccctgat ttccgatacc gtactggaac
      421 cacgccgctc ggtgacgccg gtgggtgctt ccagttccgg cagcgcgggc gctaccgata
      481 ttttcggctc cagcgaccag atggtatttt cccaaaccat cctgacggaa ctggtgtact
      541 acaagggtga caccaccttc cggccaccgg attatgaatt ccgtttcacg cctgccatca
      601 attacaaccg ggtggaactg gatgaaatcc agggcatcaa cgccgatccg gcggaaggtg
      661 acacccgtac tgatgatcat atcggcattc aggcggcgtt cgcggacgtg catttgcgca
      721 acgtgggcac ccgttatgat ttcgacagca tccgcgtggg catccagcca ttcacggcgg
      781 attttcgcgg cttcctgttc aatgacagcc cgtttggcgt gcgcctgttc ggtaaccgcg
      841 agaacaacgt gttccaatac aacctggcct ggttccgccg catggaaaaa gacaccaata
      901 gcggcctgaa cgatgtgagc gaggatctgc gtgacgacga tatttatgtc gccaacctgt
      961 actggcagga tctgggtgtg ctcggcttca cgtcgcaatt tacggtgctg tacaaccgca
     1021 accgcgaaac cgatatccaa tacgacaaca acgatttcat cgtgcggcct tcgtcgttcg
     1081 gggaagaacg gccgaaggaa tacgacgtta cttacttggg ctacagcggc gatggccaca
     1141 tcggctggct gaacctgagc accagtctgt attacgcggt gggcacggaa gatccggcgc
     1201 ctttctacgg tgacgaaacc gatatcagcg cgtttttcgc tgcggcggaa gctggtatcg
     1261 accaggactg ggtgcgctgg cgtgtgtcgg cactctatgc cagtggcgat gaagatcctt
     1321 acgacgatgt gtcgcagggg ttcgatgcga tttttgaaaa tccgctgttc gccggtgccg
     1381 ataccagtta ctggattcgc cagaatgtgc cgctggtggg cggtggccgc gttgcgctgt
     1441 cgggccgcaa tggcgtgctg aacagcctgc gcagttcgaa ggaacagggc cagtccaatt
     1501 tcaccaatcc gggcacgcag ctgctgggtg tcggtgccga tttcgatgtg ctgccgcaat
     1561 tgcggctgtc gttcaatgtg aaccagctct ggttcgacga cacggcggtg ctggaggtgg
     1621 cgcgcaacca gggcggcatc gctaccgata ttggtgtcga tgcgtcggtg gcagcgatct
     1681 ggcggccctg gatgcaccag aatgtggtgt gccgtctttc ctacgcgcaa ctgattccgg
     1741 gggagggcta ccaggatctg tatctggatg atgcatcacc ctactcgctg ctcgccaacc
     1801 tgattctgac gtactgaggc tattgcaatg aatccttcga tcaaggaagt cctggtcgct
     1861 gcagaaattc cggtcgtgcg gatgcggcta caccgacgtg cctctgtgtt cttttttgtt
     1921 gacggaaaac tgagtttttc cagcctgttt ttggtatgcg caatgctgct ggcgtgcgct
     1981 gccaacgtgc aggccagttc ctccctgggt aaaccgcggg agatccatta tcccgtttac
     2041 gcgccatcac cggccaggca ggccagcgcg gaaatggacg caaaaagcgc tggctgcctg
     2101 agctgtcaca cagctaccga tcaacccagc atgcatgcca gcgaaggcgt gaaactgggc
     2161 tgcgtggatt gtcacggcgg caacgcggcg gtgaatcgca gccagaatga tcaacccggc
     2221 agcgacggtt ataacaaaac actgaatgat gcccatgtgc agccgctgta tccgcacgcg
     2281 tggaactttc ccaacagcgc caatcccgaa cgcacctaca cactgctgaa tcaggaaagc
     2341 cccgaattta tccgtttcat caacccgtcg gattatcgtg tggtggaaga agcctgtggt
     2401 gcctgccaca tgccggtgat tgaagcgacc cggcgcagcc tgatgtcgac tggcgcgatg
     2461 ctgtggggcg gggcggcgta caacaacggc attctgccat tcaaaaatta tattctgggt
     2521 gaggcctaca cccgtgacgg cgaaccggcg agcatcacca atccggtgga gccgacgccg
     2581 gaaatgaaag cgatgggcgt gctgccggcg ctggcaccca tgccagcctg ggaaacggtg
     2641 aagccggggg atattttccg cgtgttcgaa cggggcggcc gcaatattca atcgctgttc
     2701 ccggaaacgg cactgccgaa tatcgccggc aatatccagc ggctggaaga gccgggccgg
     2761 cccgacatca agcaatccaa tcgtggcccc ggcaccggcc agcgcatctc ggtgccgctg
     2821 atcaatattc acaagacacg cctgaacgat cccttcatgt ggttcatggg caccaacgat
     2881 cagcccggtg attaccgcca ttccggttgc gccggctgcc atgtggtgta cgccaacgac
     2941 cgcgatccgc gccattccgg cccgtacgca aagttcggcc acaccggcac gacgcacact
     3001 gccgacccca ccatcgagaa agacgaaacc gggcatccgc tgcaacacag tttcacccgc
     3061 agcattccca gcagccagtg catgatctgc cacatgcacc agcccaacat gttcatgaat
     3121 accttcctcg gttacaccat gtgggattac gaatccgatg cgcccttcat gtggcccgag
     3181 aagcagaagt atcccactga tgaagaacag cgcaagattc tcgaccgcaa tccggaaggc
     3241 gcggcagtgc gcggcaagtg gggcgatctg gaattcctca agaatgtgtc cagtctgaat
     3301 ccgcaattga aggacacgca gtttgccgat taccacggcc acggctggaa tttccgcgcc
     3361 atattcaagc gcgaccgcaa gggccatctg ctggacgaga agggcaatat cgtcagtaac
     3421 gatgatccgc agaaattcaa gaaggcagtg cacatgtcgt cggtgcacgt tgacaagggc
     3481 atgcactgtg tcgattgcca cttcggtcag gatgctcacg gcaacggcca tatttatggc
     3541 gaagtggcag cggcggtgga agtggattgc gtggattgcc acggcactgc gcaggcctat
     3601 ccgtcgctga tcacctctgg cccggcggcg ctggacggcg gccaggatct gtcgctgatc
     3661 cgcaatcccg atggccagaa acggtttgaa tgggtgaacg gcaaactgat ccagcgttcg
     3721 gtggtgacgc cgggactgga gtgggaaatg tcgctggtga aagacagtgt cacgcctggc
     3781 aacgcgcact acaacgcaaa agcggcgcgc gcgaaactgg tgagcaacga caccgcgcaa
     3841 cagacctgga acggcaagac tgcgcccaac gatctggccc acagcaatga caagctggaa
     3901 tgctatgcct gccatacctc ctggaccaca tcctgcggtg gctgccacct gccgatcgaa
     3961 gccaactgga aaaccgagcg ccatcattac gaaggcggcg aaacccgcaa ttacgccacc
     4021 tacaatccgc aggtggcccg cgacgagatt ttcattctcg gcctgcgcga accgtccagc
     4081 ggtggccggg tggcgccggt gcgttccact tctgcgctgg tgctgtcatc gaccaattcc
     4141 aaccgcgaga aaatctacat ccagcaaccg cccattgcgg ccagcggtta cagctcgcag
     4201 gcgatgaacc ctcattaccc gcacacggaa cgcaagacgg aaaccaaaac ctgcagcgat
     4261 tgccacgtgg cggaggctaa cgacaacaac gcgatcatgg cgcagaccct gggtcacggc
     4321 acccagttca ttaatctggt gggttacaac gcctggctgg gcactgaaaa cagcgtcacc
     4381 gccgtgcagg tcaccgagtg ggaagaaccg caagcggtga tcggcagtta tctgcaccgt
     4441 tatgcctatc cggatttctt ccagcagcat caggacagaa acaagctgtt gccggaagcg
     4501 cacagtcaca gcagcggttc agccaattgc gtgcaactgc gcggtgaata cctgtatgcg
     4561 gcggaagggg agaagggctt ccgtatttac gacgtggcca gcattgccaa caagggtgtg
     4621 tcgcaacgca ttattaccgc gccgttcagt gcgctgggac acgacgcgca tcttgaaacc
     4681 cgtgatgcgt cctgtgtcgt gctcgccacc acgcaaccgg tgcaaccgtc gcgcaacc
//
LOCUS       DASUEG010000728         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1713590, whole genome shotgun sequence.
ACCESSION   DASUEG010000728 DASUEG010000000
VERSION     DASUEG010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713590"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..261
                     /gene="rpmI"
                     /locus_tag="VFX11_13720"
     CDS             67..261
                     /gene="rpmI"
                     /locus_tag="VFX11_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX4939732.1"
                     /translation="MPKMKTNRGAAKRFKKTANGFKRKHAFKRHILTKKSSKRIRQLR
                     PMGMVHASDVALVARMMPYV"
     gene            295..654
                     /gene="rplT"
                     /locus_tag="VFX11_13725"
     CDS             295..654
                     /gene="rplT"
                     /locus_tag="VFX11_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX4939733.1"
                     /translation="MARMKRGVVARRRHKKILKAAKGYYGARSRVLRVAKQAVTRAGQ
                     YAYRDRRQRKRQFRQLWIVRINAAARMNGMSYSRFINGLKKAAIEIDRKVLADIAVHD
                     LATFAALAEKAKASLSS"
     gene            765..1781
                     /gene="pheS"
                     /locus_tag="VFX11_13730"
     CDS             765..1781
                     /gene="pheS"
                     /locus_tag="VFX11_13730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX4939734.1"
                     /translation="MENLQTLLQQALSAVAQAESVQALDQVRVQYLGKKGEITDQLKN
                     LGKLAPEDRPAAGAEINRAKDAVQLALKERQMLLENAALDQQLAREGVDVTLPGRRRT
                     VGGLHPVTRTLQRIESFFANMGFQVVEGPEIEDDYHNFEALNIPSHHPARAMHDTFYF
                     DASTLLRTHTSPVQVRVMEQGGAPYRIICPGRVYRCDSDLTHTPMFHQVEGLLVDTNI
                     SFADLKGMLAIFLRDFFEKELDVRFRPSYFPFTEPSAEVDIQCVMCNGKGCRVCKHTG
                     WLEVLGCGMVHPKVFEHVGVDTEKFTGFAFGMGVERLTMLRYGVNDLRLFFENDLKFL
                     RQFR"
     gene            1824..>2070
                     /locus_tag="VFX11_13735"
     CDS             1824..>2070
                     /locus_tag="VFX11_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX4939735.1"
                     /translation="MRFSEKWLREWVNPSITTAELVAQLTMAGLEVDSAEPVAGQFSG
                     VVVAEILSAERHPDAEKLQVCTVNVGAGETLSIVCGAA"
BASE COUNT          464 a          580 c          611 g          415 t
ORIGIN      
        1 aagaaaaagt aagccgtccg gcttgcttgt cgtttacaac attgtgcaaa caatgcggag
       61 cagaacatgc ctaagatgaa aaccaaccgt ggcgcggcca agcgtttcaa gaaaaccgcc
      121 aacggcttca agcgtaaaca cgccttcaaa cgtcacattc tgaccaagaa gagttccaag
      181 cgtatccgtc aactgcgtcc catgggaatg gtgcatgcat ccgacgtcgc tctggtagct
      241 cggatgatgc cctacgtata acggtctgat tgaacttttt ggaggtaatg aaccatggca
      301 cgtatgaaac gcggcgtagt cgccagaaga cgccacaaga aaatcctgaa ggccgctaaa
      361 ggctattacg gcgcacgcag ccgcgtgttg cgcgtagcca agcaggccgt tacccgcgcc
      421 ggacagtatg cgtaccgcga ccgccgtcag cgcaagcgtc agttccgcca gctgtggatt
      481 gtccgtatca acgccgccgc gcgtatgaac ggcatgtcct acagccgctt catcaatggc
      541 ctgaaaaaag ctgccattga aatcgatcgt aaagtactgg ccgacatcgc agtacacgat
      601 cttgctacct ttgcagcatt agcagaaaag gcaaaagcca gcctgtccag ctaagcgtag
      661 cgggttcgcc gtcatctcga cgtgatgaat cgtgcataga ataggggaag ggctcgcgct
      721 cttccccttt ttgttttttg gtgactgaca ctggcggatc actcatggaa aatctgcaaa
      781 cccttctcca gcaagcactg tctgcggtgg cgcaggcgga gtccgtgcag gcgctcgatc
      841 aggtgcgggt gcagtatctg ggcaagaaag gggaaattac tgaccagctg aaaaatctcg
      901 gcaagctggc gccggaagac cgccccgcag cgggcgccga aatcaatcgc gcgaaagacg
      961 ccgtgcaact ggcgctgaaa gaacggcaga tgctgctgga aaatgccgcg ctggatcagc
     1021 aactcgcccg cgaaggtgtg gacgtgaccc tgccggggcg tcgtcgcacg gtcggcggcc
     1081 tgcatccggt gacgcgcacc ctgcagcgca tcgaatcttt cttcgcgaac atgggcttcc
     1141 aggtggtgga aggcccggaa atcgaagacg actaccacaa tttcgaagcc ctcaatattc
     1201 cgtcccatca cccggcgcgg gcgatgcacg acacgttcta ttttgatgcg tccacgttgc
     1261 tgcgcacgca cacctcgccg gtgcaggtgc gggtgatgga gcagggcgga gcaccctatc
     1321 gcatcatctg ccccggccgc gtttaccgct gcgattccga tctgacgcac acgcccatgt
     1381 tccaccaggt ggaggggctg ctggtggaca cgaatatcag cttcgccgat ctgaaaggca
     1441 tgctggcgat tttcctgcgg gatttcttcg agaaggaact ggatgtgcgg ttccgcccgt
     1501 cctatttccc gttcaccgag ccgtcggcgg aagtggatat ccagtgcgtg atgtgcaacg
     1561 gcaagggctg ccgcgtctgc aagcacaccg gctggctgga agtgctgggc tgcggcatgg
     1621 tgcatccaaa ggtattcgag catgtcggtg tcgatacgga aaaattcacc ggctttgcat
     1681 ttggcatggg cgtcgagcga ctcaccatgc tgcgttatgg cgtaaacgac ctgcgcttgt
     1741 tctttgagaa cgatctgaag tttctcaggc agtttcgtta atcacttcca acgcgtcgat
     1801 cacagaattt caggggaacc aacatgcgtt tcagcgaaaa atggcttcgt gaatgggtga
     1861 atccttccat caccacggcg gaactggtgg cccagctcac catggccggt ctggaagtgg
     1921 acagtgccga accggtggcg gggcagttca gtggtgtcgt cgtggcggaa atcctgtccg
     1981 ccgagcggca tcccgacgcg gaaaaactgc aggtgtgcac agtgaatgtg ggcgccggcg
     2041 agacactgtc catcgtgtgc ggtgccgcca
//
LOCUS       DASUEG010000729         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1864432, whole genome shotgun sequence.
ACCESSION   DASUEG010000729 DASUEG010000000
VERSION     DASUEG010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864432"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFX11_13740"
     CDS             complement(<1..648)
                     /locus_tag="VFX11_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939736.1"
                     /translation="MADEGELTREQIRQISEFINRGLQTSRDRMHALGMAEDALLDPD
                     ENLDRQVVKLRALYLEAEKAALAFDSRSEHFWRMLQMRLQPVVLAYSTHEKFIQNLDQ
                     DALELRSDLLMEIYRERLDNATRFRQIFFELDDRVKQESTQLKTAAPVTAPAPAHGER
                     LQKVADDSAAIQGNVLKMLERQLRDMEKAFNKKHESLQLANEEILQLKDGIQEKDK"
     gene            complement(737..>2153)
                     /gene="rlmKL"
                     /locus_tag="VFX11_13745"
     CDS             complement(737..>2153)
                     /gene="rlmKL"
                     /locus_tag="VFX11_13745"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEX4939737.1"
                     /translation="SAADRSEQMEFAIRGYDADLYAIQAAGRNVKAAGLEGCIHLERR
                     ELAHFAVHEKNAAAGGFVVCNPPYGERLDKDSDLIHLYRAFARKLQRYCLQWQVALIT
                     NQIEYADVLQLDEPQTHRVFNGPIRCFIRSGIVVPRTAQFVPVPLQLSQQDFSSAPAR
                     DLVNRIRKNVKQLDKWVQKEDVYAYRVYDADIPEYNLAVDWYNGQLHVQEYAPPKTVD
                     ADKAAKRLDDALDALKALFELPPSRIHVKSRQRQKGNQQYQKLNDQKRTYIIEEAGAL
                     LLTNFDDYLDTGLFLDHRPTRIRLQQLAKGKRFLNLFCYTGAATVHAAVGGAKKTVSV
                     DMSATYLQWARSNLYLNGFAEASHQLIRSDCMTWLRNTNDQFDLIFVDPPTFSNSKRM
                     QGHFDVQAAHEELLDLVMKRLEPGGLVIFSNNFRGFKLAAGLADKYGVKDITQASLPP
                     DFARGKPIHQCWELTLKPVAD"
BASE COUNT          392 a          638 c          629 g          494 t
ORIGIN      
        1 cttgtccttc tcctgtatgc cgtctttcag ctgcaggatt tcctcgtttg ccagctgcag
       61 cgattcatgc tttttgttga aggccttttc catgtcacgc agttgacgct ccagcatttt
      121 cagtacattg ccctgaatcg ctgcactgtc gtcggccacc ttttgcaggc gttcgccatg
      181 cgcaggtgct ggcgcggtga ccggcgccgc cgttttcaac tgggtgcttt cctgtttgac
      241 gcggtcatcc agctcgaaaa agatctggcg gaagcgggtc gcattgtcca gccgctcgcg
      301 gtagatctcc atgagcagat cggagcgcag ttccagcgcg tcctgatcca ggttctgaat
      361 gaatttttca tgggtgctgt aggccagcac aacgggctgc aggcgcatct gcagcatgcg
      421 ccagaaatgt tcgctgcggg aatcgaatgc cagggctgct ttttcggcct caagatacag
      481 ggcgcgcaac ttcaccacct ggcgatccag gttttcatcc gggtccagca acgcatcctc
      541 ggccatgcct aatgcatgca tgcgatcgcg cgaggtctgc aacccccgat tgatgaattc
      601 gctgatctgg cggatctgtt cgcgggtgag ttcaccctcg tctgccatac cctgctccac
      661 cggcattaac tctccctgaa agcctagcag agaaatgcaa agcccctgat tacgctggtg
      721 gtaatactgg cctggatcaa tcggcgacag gcttgagggt cagctcccag cactggtgga
      781 tgggcttgcc ccgggcaaaa tccggcggca ggctggcctg ggtgatgtcc ttcacgccat
      841 atttgtccgc cagcccggcc gccagcttga atcccctgaa attgttggaa aaaatcacca
      901 gtccaccggg ctccagccgc ttcatgacca gatccagcag ctcttcgtgt gcggcctgca
      961 catcgaaatg cccctgcatg cgcttggagt tggagaaggt gggcggatcg acaaagatca
     1021 ggtcgaactg atcgttggta ttgcgcagcc aggtcatgca atcgctgcgg atcagctggt
     1081 ggctggcctc ggcaaagccg ttcaggtaga gattgctgcg cgcccattgc aggtaggtgg
     1141 ccgacatgtc caccgacacg gtctttttgg cgccgcccac cgccgcgtgc acggtggcag
     1201 ccccggtgta gcagaacagg ttcagaaaac gcttgccctt cgccagctgc tgcagccgga
     1261 ttcgggtagg ccggtgatcc agaaacagac cggtatccag gtaatcgtcg aaattggtca
     1321 gcagcagcgc gcccgcttct tcgatgatat acgtgcgttt ctggtcgttc agtttctggt
     1381 actgctggtt gcccttctgg cgctggcggc ttttcacatg aatgcgcgac ggcggcagtt
     1441 caaacagcgc tttgagggca tccagtgcgt catccaggcg cttggccgcc ttgtctgcat
     1501 ccacggtttt cggcggtgcg tattcctgca catgcagctg gccgttgtac cagtccaccg
     1561 ccagattgta ttcgggaata tcggcatcgt agacgcgata ggcatacacg tcttccttct
     1621 gcacccactt gtccagctgt ttcacgttct tgcggatgcg gttcaccaga tcgcgtgcag
     1681 gcgcactgga aaaatcctgc tggctcagtt gcagcggcac cggcacgaat tgcgcagtgc
     1741 gcggcaccac gatgccactg cggataaaac agcggatcgg gccgttgaac acgcggtggg
     1801 tttgcggttc gtccagctgc agcacgtcag cgtattcgat ctggttggtg atcagcgcca
     1861 cttgccactg cagacaatag cgctgcagtt tgcgggcgaa ggcgcgatac aggtggatca
     1921 ggtcgctgtc cttgtccagg cgttcgccgt agggagggtt gcacaccacg aaaccgccgg
     1981 cagcggcatt tttttcatgc acggcgaaat gagccagttc gcgccgctcc aggtggatgc
     2041 agccttccag tcccgctgcc ttgacgttgc gacccgctgc ctggatcgca tacagatcgg
     2101 cgtcgtaacc gcggatggca aattccatct gttccgaccg gtctgcagcg ctc
//
LOCUS       DASUEG010000730         4467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2177599, whole genome shotgun sequence.
ACCESSION   DASUEG010000730 DASUEG010000000
VERSION     DASUEG010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177599"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1506)
                     /locus_tag="VFX11_13750"
     CDS             complement(25..1506)
                     /locus_tag="VFX11_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4939738.1"
                     /translation="MSVWSKLVRGHLAALSESCSDEVIDFMLMESGLARDKSGEIQAD
                     SFADLNRYLDTLENYLGLLYRERAEKIFSDVIEQGWQGAENRPELDASFHAELEQKAK
                     LAKKLESLNKDLLQAKESAEVASRAKSVFLASMSHEIRTPMNGVIASVQLLQDTRLDR
                     EQNELVSLIWRSSNALLNIINDILDLSKIEAGHLQLNREAFNLRQLIADVSDLNRATA
                     DDKQVAINVTVEDGTPPYVLGDPVRVRQVLINLVSNAIKFTREGHVNVEVGYDRIDDE
                     RYNVIIEISDTGIGIPKDRIEKIFESYEQADASIGRNYGGTGLGLTICKSLVDLMDGY
                     VEVESETGKGASFIITLPFRVAEMPREEREIPGAPKNYRKKVLVAEDNVVNQRVAQKM
                     LTRVGIDADIVFDGEAAIEAASNRQYDLIMMDIEMPVVDGIEATRVIQAGNGPNKKTP
                     ILALTASVLDDDRKRILAAGMQGFVGKPVRLNQLTQELDRWFN"
     gene            1842..2546
                     /locus_tag="VFX11_13755"
     CDS             1842..2546
                     /locus_tag="VFX11_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939739.1"
                     /translation="MHPDSVPIEPAATLVLLQDSPAGPQVLMQQRSHDAQFVGGAWVF
                     PGGKLDPHDNDPHWLRHCDIGPDRANRLLGTDSGALAYWVAALRESVEEAGLLLARQQ
                     GEPVSLTLAAEAQHFLHRRRDGFLDFCRDHSLTLCTGDLRYLSRWVTPPGLPRRYDAR
                     FFMAATPVGQHPQQDDHEAINTCWVTPEQALEHYRNNEWMLVLPTLVTLRQLSGYESV
                     AAILSQLGNHIDAKTG"
     gene            2599..3441
                     /locus_tag="VFX11_13760"
     CDS             2599..3441
                     /locus_tag="VFX11_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4939740.1"
                     /translation="MSSFIPGEVVVLSQRVRRITAPNPGMMTGPGTNTYLVGNRELAV
                     IDPGPSSREHIEAIVRTADSMHAPIRWILCTHTHLDHSPGANLLRDLTDASVIGQVAR
                     HPNAQDPLFTPDQHWQHGDFLQTDEFTLRAIHTPGHASNHLCFLLEEEELLFTGDHIM
                     EGSTVVIAPPDGDMKAYIDALHGLKQLPLRHLAPAHGNRIDNPQQLIEALIWHRKMRE
                     NKTLDRMRQLGPCSLETLTPSVYDEVPSFLHGIARMSLLAHLLKLEQEQTVRQQDGHW
                     RISD"
     gene            3451..4413
                     /locus_tag="VFX11_13765"
     CDS             3451..4413
                     /locus_tag="VFX11_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4939741.1"
                     /translation="MRTIILDAASLGSDITLEPLQRAAGDFIRHDYCDPEQAVERLQS
                     ADIAIVNKTVLTGAILAQCPQLKMIAVTATGTNNIDLKAAQARRIRVCNVTRYGRASL
                     VQHTFMLMLALAGNLPAYQRDVKTGRWNSAANFCLMDHRILELEGKTLGIVGYGDLGH
                     GVAQMARGLGMHVLASRRGDQSNTTDDGIPRLPLHDLLPQVDVLSLHCLLTDANRNLI
                     GTTELGLMKPTAFLINTARGGLVDEAALATALKNGKLGGAATDVLSQEPPVNGNVLLD
                     LDLPNLIITPHCAWASQEARQRLINLTADNIHNFINGTLKRYVV"
BASE COUNT          883 a         1505 c         1253 g          826 t
ORIGIN      
        1 atgaaaaacg gttgccggca tgcttcagtt gaaccagcga tccagctcct gggtcagctg
       61 gttcaggcgc accggcttgc cgacgaatcc ctgcatgccc gccgccagaa tgcgcttgcg
      121 gtcatcgtcc aggacgctgg cggtgagggc gagaatgggg gtcttcttgt tgggaccatt
      181 gcccgcctga atcacccggg tggcttcaat gccatccacc accggcattt cgatgtccat
      241 catgatcaga tcgtactggc ggttgctggc ggcctcgatg gcggcctcgc cgtcgaatac
      301 gatgtcggcg tcgattccca cccgggtcag catcttctgc gccacccgct ggttcaccac
      361 gttgtcttcg gccaccagca cttttttgcg atagtttttc ggcgcaccag ggatttccct
      421 ttcttcccgt ggcatctccg ccacccggaa tggcagcgtg atgatgaacg atgcaccctt
      481 gccggtttcg ctttccactt ccacgtaacc atccatcaga tccaccaggc tcttgcagat
      541 ggtgaggccc agtcccgttc ccccgtaatt gcggccaatg gaggcgtccg cctgctcgta
      601 ggattcgaaa atcttttcga tgcgatcctt gggaatgcca atgccggtgt cgctgatttc
      661 gatgatcacg ttatagcgtt cgtcgtcgat gcggtcgtaa cccacttcca cattgacatg
      721 gccttcgcgg gtaaacttga tggcgttgga caccagattg atcagtacct gccgcacccg
      781 caccggatca cccagcacgt acgggggggt gccgtcctcg acggtgacat tgatggcgac
      841 ctgcttgtcg tcggcggtcg cgcggttcag atcgctgaca tcggcaatca gctgacgcag
      901 gttgaaggcc tcgcggttca gttgcagatg ccctgcctcg atcttggaca ggtcgagaat
      961 gtcgttgatg atgttcagca acgcattgga ggaacgccag atcaggctca ccagctcatt
     1021 ctgttctctg tcgagacggg tgtcctgcag caactgcaca ctggcgatca cgccattcat
     1081 gggggtgcgg atctcgtggc tcatgctggc cagaaagacg gatttggcgc gcgaggccac
     1141 ttccgccgac tctttggcct gcagcaaatc cttgttcagg ctttccagct tttttgccag
     1201 tttggctttc tgttccaatt cagcatgaaa gctcgcatcc agttccggcc ggttctcggc
     1261 gccctgccag ccctgctcga tcacgtcact gaagatcttt tccgcccgct cccggtacaa
     1321 aagccccagg tagttctcga gggtatccag ataacggttg agatcggcaa aggaatcggc
     1381 ctgaatttcg ccactcttgt cgcgggccag cccgctctcc atcagcatga aatcgatgac
     1441 ttcatcactg caggattcgc tgagggcggc cagatggcca cgcaccaatt tggaccagac
     1501 ggacatagga aaggaggtct cttgactgaa ctgcctgatt aaaagcctaa caacagaacc
     1561 cctatccggc aaaccggccg gtctatcagc cgccagacat aaccggagcg tctaagagaa
     1621 gatattttag ggcgttttca ttgcagtgac ggggacctgg agcatgatgg agccggcatt
     1681 gaccgggcga tcccaggcag acctcccccg tgtcgcaatt gaaggccgtg aacaaacggg
     1741 cagcctcgtg ggcaggagaa ccggttgccc ccgtccgcct gattgggtcc tcccatctgt
     1801 taaagtgtag acccgcaatc cacctgatcg agcgagttgc cgtgcatcct gattccgtcc
     1861 ccatcgaacc tgctgccacc ctggtcctgt tgcaggattc acctgccggc ccccaggtgc
     1921 tgatgcagca acgcagccat gatgcgcagt tcgtcggcgg cgcctgggtg tttcccggtg
     1981 gcaagctgga cccccatgac aacgatccgc actggctcag gcactgcgac atcggcccgg
     2041 accgggccaa ccgcctgctg ggcaccgaca gcggcgccct ggcgtactgg gtagcggcgc
     2101 tgcgggaatc ggtagaggaa gcgggactgc tactggctcg gcaacagggc gaaccggtca
     2161 gcctgacgct ggcagccgag gcccagcact tcctgcaccg gcgccgggac ggcttcctgg
     2221 acttctgccg ggaccacagc ctcacactct gcaccggcga tctgcgctac ctgagccgct
     2281 gggtcacgcc gcccggcctg ccccggcgct acgacgcgcg cttcttcatg gcggccacgc
     2341 ctgtcggcca gcacccgcaa caggacgatc acgaagcgat caacacctgc tgggtcaccc
     2401 cggagcaggc gctggaacat taccgcaata acgaatggat gctggtgctt cccaccctgg
     2461 tcactctgcg tcagttaagc ggctacgaat ccgtggcggc aatcctgagt cagctcggga
     2521 atcacatcga cgccaaaacg gggtaaaatc cagccttcac tccttcaaac ccgcaacaag
     2581 acctgcgagc atcctcccat gtccagtttc attcccggcg aagtcgtagt attgagccag
     2641 cgggtacgtc gcattaccgc gcccaacccc ggcatgatga ccggtcccgg caccaacacc
     2701 tatctggtgg gcaaccgcga actggcagtg atcgaccccg gcccctccag ccgcgagcac
     2761 atcgaggcca tcgtccgcac tgccgattcc atgcacgcgc cgatccgctg gatcctgtgc
     2821 acgcacacgc atctggacca ctcccccggc gccaatctgc tgcgggatct caccgatgcc
     2881 agcgtgatcg gccaggtggc ccgccacccc aacgcccagg acccgctctt cacacccgac
     2941 cagcactggc aacacggcga tttcctgcag accgacgaat tcaccctgcg cgccatccac
     3001 acccccggcc atgcctccaa ccacctgtgt ttcctgctgg aagaggaaga actgctgttc
     3061 accggcgacc acatcatgga aggctccacc gtggtgatcg cgccgccgga cggcgacatg
     3121 aaggcctaca tcgacgccct gcacggcctc aagcaattgc cgctgcgcca cctggcgccg
     3181 gcccacggca accgcatcga caacccgcag cagctcatcg aagccctgat ctggcaccgc
     3241 aaaatgcgcg agaacaaaac gctggaccgc atgcgccagc tgggcccctg cagcctggaa
     3301 acgctgacgc catcggtata cgacgaagtg ccctccttcc tgcacggcat cgcgcgcatg
     3361 tcgctgctgg cccacctgct gaaactggag caggaacaga ccgtgcggca gcaggacgga
     3421 cactggcgaa tttccgacta aactttgatc atgcgaacca ttattctcga cgccgccagc
     3481 ctgggcagcg acatcaccct cgaacccctg caacgggccg ccggggactt catccgccat
     3541 gactattgcg atccggagca ggcggtggaa cgcctccagt ccgccgacat cgccatcgtc
     3601 aacaagacgg tgctgaccgg cgccatcctg gcccagtgtc cgcagctgaa aatgattgca
     3661 gtgaccgcca ccggcaccaa caacatcgac ctgaaagccg cgcaggcccg ccgcattcgt
     3721 gtctgcaacg tgacccgtta tggccgggca tcgctggtgc agcacacgtt catgctgatg
     3781 ctggccctgg ccggcaacct gcccgcctac cagcgcgatg tcaaaaccgg gcgctggaac
     3841 agcgccgcca acttctgcct gatggatcac cggattctgg aactggaagg aaaaaccctg
     3901 gggatcgtcg gttacggcga cctgggacat ggcgtggcgc agatggcgcg gggactgggc
     3961 atgcacgtgc tggcatcccg gcgcggcgac cagtccaaca caaccgatga cggcatcccg
     4021 cgcctgcccc tgcacgatct gctgccgcag gtggacgtgc tgagcctgca ctgcctgctg
     4081 accgatgcca accgcaacct gatcggcacg accgaactgg gcctgatgaa gcccactgca
     4141 ttcctgatca ataccgcccg tggcggactg gtggacgaag ccgcgctggc cacggccctg
     4201 aagaatggca aactgggcgg tgccgccacc gacgtgctgt cacaggaacc gccggtgaac
     4261 ggcaacgtat tgctggatct cgacctgccc aacctgatca tcacccccca ctgcgcctgg
     4321 gccagtcagg aagcccgcca gcgcctgatc aacctcaccg ccgacaacat ccacaacttc
     4381 atcaacggca cgctcaagcg ctacgtggtt tgagacgctg accgcaacag atcccgtggc
     4441 atgaaatgcc agtcccgccc ggccaaa
//
LOCUS       DASUEG010000731         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1605765, whole genome shotgun sequence.
ACCESSION   DASUEG010000731 DASUEG010000000
VERSION     DASUEG010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605765"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFX11_13770"
     CDS             complement(<1..752)
                     /locus_tag="VFX11_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4939742.1"
                     /translation="MTDTLFPVADEGSEQVALRTFTEQAYLNYSMYVIMDRALPHIGD
                     GLKPVQRRIVYAMSELGLKNTAKYKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFS
                     YRYPLVDGQGNWGSPDDPKSFAAMRYTESRLSPYAEVLLSELEQGTVDWQPNFDGTME
                     EPRVLPARLPNLLLNGTTGIAVGMATDVPPHNLKEIARACIALLEDPGISLDELCEFV
                     PGPDLATAAEIITPRHELVQMYATGKGGFRMRA"
     gene            950..1591
                     /locus_tag="VFX11_13775"
     CDS             950..1591
                     /locus_tag="VFX11_13775"
                     /inference="COORDINATES: protein motif:HMM:NF022168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
                     /protein_id="HEX4939743.1"
                     /translation="MSRVTLSTTPPITSGGEKLVFWHPGQPGHLIKVINTRYFDYMHK
                     QWPVMMRLQRLAHYWSYLRELTEHIAARANGVPDLHHLQNITGLVDTDLGLGLVTEAV
                     TRPDGQLAWTLGDLIEQGHYGPVEQAAFERFCQWLSNAPLIIRDLSVHNLVWHEAGEY
                     FVVIDGVGGKIRPTLRSFSDYYNRRSNRQKVAKLKERIARMQAKQPGIRHASA"
     gene            complement(1593..2745)
                     /locus_tag="VFX11_13780"
                     /pseudo
     CDS             complement(1593..2745)
                     /locus_tag="VFX11_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
BASE COUNT          543 a          888 c          797 g          517 t
ORIGIN      
        1 gcgcgcatgc ggaagccgcc cttgccggtg gcgtacatct gcaccagctc gtgacgcggc
       61 gtgatgattt cggcagccgt cgccaggtcc gggccgggga caaattcgca gagctcgtca
      121 agcgaaatgc ccgggtcttc cagcagggcg atacaggcac gggcaatttc cttcaggttg
      181 tgcggcggca cgtcggtggc catgcccacg gcaatgccgg tcgtgccgtt cagcagcagg
      241 ttgggcaggc gcgccggcag caccctgggt tcttccatgg tgccgtcgaa gttgggctgc
      301 cagtccaccg tgccctgctc cagttcggag agcaagacct cggcataggg tgacaggcgc
      361 gattcggtat aacgcatggc ggcaaaggac ttgggatcat ccggtgagcc ccagttaccc
      421 tggccatcca ccagcgggta gcggtagctg aaaggctgcg ccatcagcac catggcctcg
      481 tagcaggcac tgtcgccatg cgggtggtac ttacccagca cgtcaccgac ggtacgggcc
      541 gacttcttgt acttggccgt gttcttgagg cccaactcgc tcatcgcata gacgatgcgg
      601 cgctgtacag gcttgagacc gtcaccgata tgcggcaagg cgcggtccat gatcacgtac
      661 atggagtagt tcaggtaggc ctgctcggta aaggtccgca gggcgacctg ttcggagcct
      721 tcatcggcaa ccggaaacag cgtgtcagtc atgaaaatgg cgtctgcatg aagatgaaca
      781 acccgggcga tggtagacca gcccgccacg gtcggcgagc ccccggcccc atcaccttgg
      841 gacgaagttt gcggaatgat gaccgcatga taaaattttt cttaagcttt ccttaagcta
      901 acgtttcctg cctatcccct ttcttgcttg cgccagcagg atgtccgcca tgtcacgagt
      961 caccctgagt acaacccccc ccatcacgtc cggcggggaa aagctggtgt tctggcatcc
     1021 cgggcagccc ggccacctga tcaaggtcat caacacccgc tatttcgact acatgcacaa
     1081 gcagtggccg gtgatgatgc gtctgcaacg tctggcccat tactggagct acctgcgcga
     1141 gctcaccgag cacatcgccg cccgtgccaa tggcgtgccc gacctgcacc acctgcagaa
     1201 catcacagga ctggtcgaca ccgacctcgg actgggactg gtcaccgaag cggtcacgag
     1261 gcccgatggc cagctggcct ggaccttggg cgacctcatc gaacaagggc actacggccc
     1321 cgtcgaacag gccgccttcg agcgtttctg ccaatggctc tccaacgccc cgctcatcat
     1381 ccgtgacctg tcggtgcaca acctggtctg gcatgaagca ggcgagtact ttgtggtgat
     1441 cgacggggtc ggcggcaaga tccgtcccac actgcgctcc ttctccgact actacaaccg
     1501 tcgcagcaac cgccagaagg ttgccaagct caaggagcgc atcgcccgga tgcaggcaaa
     1561 acagcccggc atccgccacg ccagcgcctg actcagactt ccaccaccgc caggttgccc
     1621 ttggtttcca gccaggtctt gcggtcgccc gcacgcttct tggccagcaa catgtccatc
     1681 atttcggacg tcagcttgtc atcttccagc atcagcttga ccagacgacg cgtatccggc
     1741 gccatggtgg tttcacgcag ctgcagcgga ttcatttcac ccaggccctt gaagcgctgc
     1801 acgtttggct tgccgcgttt gtgctcggcc tccagacggt cgaggatgcc gcgcttctcg
     1861 tcttcgtcca gcgcgtaata aacctccttg ccgaggtcga tgcggtacaa cggaggcatg
     1921 gccacgaaaa tgtggccgcg ctccaccagc ggacggaaat gtttcacgaa cagggcgcag
     1981 agcagcgttg cgatatgcaa accgtcggag tcggcgtcag cgaggatgca gatctagccg
     2041 tagcgcaggc catccagatt gtccgaggcc ggatcgacgc caatggcaac agcgatgtcg
     2101 tgcacttcct gcgatcccag aatctcgccg gaatccactt cccaggtatt gaggatcttg
     2161 ccgcgtagcg gcatcaccgc ctggaattcg cgatcacgcg cctgcttggc cgaaccgccg
     2221 gcggaatcgc cctccaccag aaagagttca gaacgggtaa tgtcggagag cgaacaatcc
     2281 gccagcttgc cgggcaatgc cggaccagag gtgatcttct tgcgcgccac ctgcttgctc
     2341 tgacgcaaac gcttttgggc attgctgata gcgagctctg ccagctgctc cgccacatcg
     2401 gtgtgctggc cgagatagag tgcaaaggca tctttcacca cacccgccac aaagctggcg
     2461 gattcacgcg atgacaggcg ctccttggtc tgcccggcaa actgcggatc ctgcatcttc
     2521 agtgagagta cgaaggcaca gcggtcccac acgtcttccg gtgacagctt cacaccgcgc
     2581 ggcaacaggt tgcggtattc gcaaaagtca cgcagcgcat cgagcagacc ggtacgcagg
     2641 ccgttcacat gcgtgccccc ctgcgcggtg ggaatcaggt tcacatacga ttcctgcacc
     2701 agctcgccac cttcgggcag ccactgcaag gcccagtcca ccgct
//
LOCUS       DASUEG010000732         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_748530, whole genome shotgun sequence.
ACCESSION   DASUEG010000732 DASUEG010000000
VERSION     DASUEG010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748530"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFX11_13785"
     CDS             <1..679
                     /locus_tag="VFX11_13785"
                     /inference="COORDINATES: protein motif:HMM:NF042820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix domain-containing protein"
                     /protein_id="HEX4939744.1"
                     /translation="MLYLPPGVAHYGVAQNDCMTLSVGFRAPSEQDILMEFVHTLFND
                     NVQDRRYEDKDLKLQANPGWISPEAVERVAGILRRAVSDPQKISRWMASYLSQTKYEQ
                     APEPPEDDYSEVEVQDLLAGNQSLRKEEASRFLYTGDVLRPEQFFINGEEVEIPPLTH
                     SMILYLCKHRSYRPATLAMFCRTPENLRFLTGLLNQGILYFEDDLYDEDEHEEEEDEE
                     TGAADDL"
     gene            723..1244
                     /locus_tag="VFX11_13790"
     CDS             723..1244
                     /locus_tag="VFX11_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4939745.1"
                     /translation="MSIQNLAPLLVVGESTGTILLESADDFRCAINRMAQQAVRSIRI
                     FTQDLDHELYDHTEFLKIASAVCRQRRNCTIQVLLKNADKAARFGHRLVELQKRLPSS
                     IEIRSVPAEYEEANDEFFLVDDIGLVKRFTLGYMRGHCEFRSVPDANKKARWFSEIWE
                     RSEPCQELRRLNL"
     gene            1241..2116
                     /locus_tag="VFX11_13795"
     CDS             1241..2116
                     /locus_tag="VFX11_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939746.1"
                     /translation="MTHLFQRLLLVLTLCLLNLPAWAEEQVSEIYETYGPAEKLVPVL
                     QPLLGVNDRITAYHNKLLVKAPRDRQDKLLEMLQEIDRPLRNLLISVRYSNLTGRELD
                     HTSTDVTYIHDNKGVRIQGQDQPDDRMVVYKGSSRDDKIQAQVVTKEQYSTRKEDVTQ
                     QIRVMEGQQGFLQVGEERPETRYVFLNPAYGVGATTEYRQVGNGVYVVPQIMRDRKIR
                     LELFTTNQRRSPSNQQRVQTTEAQSVLLVEPDVWTPFAGSSATVTTSSDSIGTSTRSL
                     KQGSPGLELKVTILE"
     gene            2248..>2426
                     /locus_tag="VFX11_13800"
     CDS             2248..>2426
                     /locus_tag="VFX11_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939747.1"
                     /translation="MTERIKKGGLEIATVLYNLVANEVAPGTGVEPDAFWSKFEQILA
                     DLAPKNKALLQKRDA"
BASE COUNT          570 a          752 c          686 g          418 t
ORIGIN      
        1 catgctgtac ctgccgccgg gcgttgccca ctatggcgtg gcgcagaacg attgcatgac
       61 gctgtcggtg ggtttccgcg cgcccagtga acaggacatc ctgatggagt tcgtccacac
      121 cctgttcaac gacaacgtgc aggaccgccg ctacgaagac aaggacctga aactgcaggc
      181 caacccgggc tggatttcgc cggaagccgt ggagcgggtg gccgggatcc tgcgccgcgc
      241 ggtgtccgat ccccagaaaa tttcccgctg gatggcctcc tatctgtcgc agaccaagta
      301 cgaacaggcg ccagagccac cggaagacga ttacagcgaa gtggaagtgc aggacctgct
      361 ggcgggcaac cagtccctgc gcaaggaaga agccagccgc ttcctctaca ccggcgatgt
      421 gctgcggccg gagcagttct tcatcaacgg cgaagaagtg gaaattccgc cgctgaccca
      481 ttcgatgatc ctgtacctgt gcaagcaccg cagctaccgg cccgccaccc tggccatgtt
      541 ctgccgcacg ccggaaaacc tgcgcttcct taccggactg ctcaaccagg gcattctcta
      601 cttcgaggac gacctctatg acgaggatga acatgaagaa gaggaagacg aagagactgg
      661 cgccgccgac gacctgtgac cgcggtccgg gccttttgcc gaatcggtgt taaactcagg
      721 ctatgtcgat ccagaatctt gcccctctgc tggtggtcgg cgaaagcacc ggaaccatcc
      781 tgctggaaag cgccgacgat tttcgctgcg ccatcaatcg catggcgcag caggccgtgc
      841 gcagcatccg gatattcacc caggatctgg accacgaact ctacgatcac acggagttct
      901 tgaagattgc ctccgccgtg tgccggcagc gccgcaactg cactatccag gtcctgctga
      961 aaaatgccga caaggcagcg cgcttcggcc accgtctggt ggaactgcaa aagcgcctgc
     1021 cctcctctat tgaaatccgc agcgtgccgg cagagtacga agaagccaac gacgaattct
     1081 ttctggtgga cgacatcggc ctggtgaagc ggtttaccct gggttacatg cgcggccatt
     1141 gcgaattccg ttccgtgccc gacgccaaca agaaagcccg ctggttcagc gaaatctggg
     1201 aacgcagcga accctgccag gaattgcgga ggctgaatct gtgacgcatt tattccaacg
     1261 tctgttgctg gtgctgaccc tgtgcctgct gaacctgccg gcatgggccg aggagcaggt
     1321 atcggaaatc tatgaaacct acggaccggc ggaaaaactg gtaccggtac tgcagcccct
     1381 gctgggcgtg aacgaccgca tcaccgccta tcacaacaaa ctgctggtga aagcaccccg
     1441 cgaccgccag gacaaactgc tggaaatgct gcaggaaatc gaccggccac tgcggaacct
     1501 gctgatctcg gtgcgctatt ccaatctgac cggccgcgag ctggatcaca ccagtaccga
     1561 tgtcacctac atccacgaca acaagggtgt gcgtatccag ggacaggatc agcccgatga
     1621 tcgcatggtg gtctacaagg gctccagccg cgatgacaag atccaggcac aagtggtcac
     1681 caaggagcaa tacagcaccc ggaaagaaga tgtcacccag cagatccggg tgatggaagg
     1741 acagcaaggt ttcctgcagg tgggcgagga acggcccgaa acgcggtatg tattcctgaa
     1801 tccagcttat ggtgtcggcg ccaccaccga gtaccgccag gtaggcaatg gcgtttatgt
     1861 cgttccgcag atcatgcgcg acaggaaaat ccggctggag ctctttacca ccaaccagcg
     1921 ccgcagcccg tccaaccagc aacgcgtgca aaccaccgag gcgcaatccg tgctgctggt
     1981 ggaaccggat gtctggacac ccttcgccgg cagcagtgct actgtcacca ccagcagcga
     2041 cagcattggc accagcaccc gcagcctgaa acagggcagc ccgggcctgg aactgaaggt
     2101 caccatcctg gaataaagac ccaccagtca tatccggaaa taatttgaca gcccaaaacc
     2161 aagattgcat catccctccc ccttgacgag cgcgcccggc gcgggttagc gcttcaccag
     2221 gcaggcactt acgatagagg aaatgacatg acagagcgca tcaaaaaagg cggtcttgag
     2281 atcgctaccg ttctttacaa cctggttgcc aacgaagtcg cccccggcac cggtgtggaa
     2341 cccgatgcct tttggagcaa gttcgaacag atcctcgccg acctggcgcc gaaaaacaaa
     2401 gccctgctgc agaagcgcga cgccat
//
LOCUS       DASUEG010000733         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2034671, whole genome shotgun sequence.
ACCESSION   DASUEG010000733 DASUEG010000000
VERSION     DASUEG010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034671"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VFX11_13805"
     CDS             <1..1510
                     /locus_tag="VFX11_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acyl-CoA reductase"
                     /protein_id="HEX4939748.1"
                     /translation="NSPTLNAFNQQQILITGTTGFVGKVILEKLLRSVPTIGRIHLLI
                     RGNAANPSARERFLNEVATSSIFDTLKARHPQRFEELCNEKLRFITGELTEPNFGLSD
                     HEFQQLAQKTDLIINSAASVNFREPLDQALQTNTLSLHSIIKLAKVKKSPVVHVSTCY
                     VNGYNTGVMPEDTVPPKHGGISRHRLGYFDVEPLIAKLQHKIVSLQSRHRNKKDCEKA
                     LIDLGISESNRYGWNDTYTFTKWLGEQILAQQLQGQTLTIVRPSIVESTLQEPVPGWI
                     EGVKVADAIILAYAREKVTFFPGNKNGIIDIVPADLVANSIILGSAEALTATPAHRIY
                     QCSSSHCNPIPIKQVIKHVQDEAMQNFAEYKNLFLRKPRRPFIMVPNVVFRTVMGLAY
                     RLLTIRSNILTRVGVTVSSAKMANLETAMKLAIIFSFYTQPRYTFSNEKLCALSERMG
                     EQDQSLFPVNGALIEWDQYLQKIHLAGLDQYSLAPKKAKPKQKQVRKQTEAA"
     gene            complement(1641..2705)
                     /locus_tag="VFX11_13810"
     CDS             complement(1641..2705)
                     /locus_tag="VFX11_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4939749.1"
                     /translation="MNSVINEDKMNALLGRIVVDFGAVSMAPLVLIGDQLGLYRALAE
                     GGALTSAQLAERTETHERYVREWLNAQAASGYVEYLADSGRYRMTPEQVAALAEEDSP
                     VFVVGAFQVALAAGRSAERLAKAFRTGQGIGWHEHDHSLFHGIERFFRSSYIGHLVND
                     WIPALSGVKEKLQAGAVVADIGCGHGASTILMAQAFPKSQFVGFDYHRESVQEANERA
                     RRAGVADRCRFEVASAKDFPGRHYDLVTIFDALHDMGDPAGASQHVLTTLAPGGSWMI
                     VEPYAGDRIEENLNPVGRAYYAGSTMICTPCSLAQEVGLALGAQAGEARMREVVMGAG
                     FTRFRRATQTPFNLVYEART"
     gene            2871..3872
                     /locus_tag="VFX11_13815"
     CDS             2871..3872
                     /locus_tag="VFX11_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4939750.1"
                     /translation="MDALSELLRVIKLSGAIFFRADYSAPWCVRSPAAERFREYVATP
                     SNRVIEFHLIADGRGYVRVGEETTPLAAGDFIMIPHGDGHYMGNGSGVKPVDGEASLP
                     LLLNQNLEIADMGGGGERTRLICGYLVCEDRLIQPLLTGLPRVVRVNMRADPASELLE
                     GMIRHAVEQAAASAPGSRVIAARLAEVLFVEVLRRYLTQLPPGQTGWLAGAADPAIGR
                     ALAALHQRPEYRWSLDALAQEANLSRSALTERFSRYFDDGPMAYLAQWRLELAAESLR
                     NTSRSVLQIASEVGYDSEAAFNRAFKRRFSAPPGQYRKAWRENAAGGPAALERALQN"
     gene            complement(3924..>4283)
                     /locus_tag="VFX11_13820"
     CDS             complement(3924..>4283)
                     /locus_tag="VFX11_13820"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX4939751.1"
                     /translation="IESPARLKYFKGLQPLANAFHERYVYPTLRLCATRCLHPLYRER
                     FNLPWSAHDEARYQKLCRRLQRYTQLVPRALRYNPVAYLVMLGFHGPGLVTLQELDKI
                     EMKKKKRAEEKRVAAGG"
BASE COUNT          944 a         1335 c         1195 g          809 t
ORIGIN      
        1 taattcaccc acattaaatg cgttcaacca gcagcagatt ctgatcacag gcactacggg
       61 atttgtcggc aaagtgattc tggaaaagct gctccgcagt gttcccacca tcggcagaat
      121 ccacttgctc attcggggca atgcagccaa tccctccgca cgggaacgct ttctgaacga
      181 agtggccacc tcctccattt tcgatacgct gaaagcccga catccgcagc gattcgaaga
      241 actgtgcaat gaaaagctgc gcttcatcac cggcgaactc actgagccca acttcggcct
      301 gagcgatcat gaatttcagc agcttgccca aaaaactgac ctgatcatca actctgccgc
      361 cagtgtcaat tttcgcgaac cgctggatca ggcgctgcaa accaacacgc tcagcctgca
      421 ttcgatcatc aaactggcga aggtaaaaaa gtccccggtt gtgcatgtat ccacctgcta
      481 tgtcaacggc tacaacaccg gggtgatgcc agaagacacc gtgccaccaa aacacggagg
      541 catcagccgc cacaggcttg gctatttcga tgtggagccg ctgattgcaa aactgcagca
      601 caagatcgtc agcctgcagt cgcgtcatcg caacaaaaaa gactgtgaaa aagcgctgat
      661 cgatctgggc atctccgaat cgaaccgcta cggctggaac gacacctata ccttcaccaa
      721 atggctgggg gagcagatcc tggcgcaaca actgcaaggc cagactctga ccatcgtgcg
      781 gccttccatt gtcgaaagca cactgcagga acccgtaccc ggctggatcg aaggcgtgaa
      841 agtggccgat gccatcatcc tggcctacgc ccgcgaaaaa gtgacgttct tccccggcaa
      901 caagaacggc atcatcgaca tcgttcccgc cgatctggtg gccaacagca tcattctcgg
      961 cagcgccgaa gcactgaccg caacaccagc acaccgcatt taccaatgca gcagcagcca
     1021 ctgcaacccg attcccatca aacaggtgat caagcatgta caggacgagg caatgcagaa
     1081 tttcgccgag tacaaaaacc tgttcctgcg caaacccagg cggccattca tcatggtgcc
     1141 gaacgtagtg ttcagaaccg tgatgggact ggcctaccgt ctgctgacga tccgcagcaa
     1201 catcctgacc cgcgtgggtg taacggtttc ctctgccaaa atggcgaatc tggaaacggc
     1261 catgaaactg gcgatcatat tttctttcta cacccaaccc cgctacacct tcagcaatga
     1321 aaagctgtgc gcgttgtcgg aacggatggg agagcaggat cagtccctgt ttccagtgaa
     1381 cggagcgttg atcgagtggg accagtatct gcagaaaatt catctggcgg ggctggatca
     1441 gtattcactg gcgccgaaga aagcaaagcc gaaacagaag caggtgcgga agcagacgga
     1501 agcggcgtga attatttgca tgcttgcgct tttttttcgc gaatagatca cgtgccctca
     1561 cagttcacga gaaaacctct gacagacatc gcacagcata gcggacgaag cctgccagag
     1621 ttggtacgtg gcccgttgtc tcaggttctc gcctcataca ccagattgaa cggtgtctgg
     1681 gttgcccggc ggaagcgcgt gaatccagcc cccatcacca cctcgcgcat gcgggcctcg
     1741 ccggcctgcg cacccagcgc cagacccact tcctgcgcca gcgagcacgg tgtacagatc
     1801 atagtggaac cggcgtaata ggcgcgcccc accggattga gattttcctc gatccgatca
     1861 ccggcgtagg gttcgacgat catccagctg ccgcccggtg ccagcgtggt cagcacatgt
     1921 tgcgatgcac cagctggatc acccatgtca tgcagcgcgt caaagatcgt caccagatcg
     1981 taatgccgcc ccggaaaatc cttggcactc gctacctcga aacggcagcg gtccgcaaca
     2041 ccggcacgac gcgcccgttc attggcttcc tgaaccgatt ccctgtgata gtcgaacccg
     2101 acaaattgtg acttgggaaa ggcctgcgcc atcagaatgg tcgacgcacc gtggccgcaa
     2161 ccgatatccg ccaccactgc acccgcctgc agtttttcct tcaccccgct cagtgccgga
     2221 atccagtcat tgaccagatg gccgatatag gacgaccgga aaaagcgctc gatgccatga
     2281 aagagcgaat gatcatgctc atgccagccg atgccctggc cggtgcggaa ggccttggcc
     2341 agacgttctg cgctgcgacc cgccgccagc gccacctgaa acgcgcccac cacaaaaacc
     2401 gggctgtcct cttccgccaa tgctgccacc tgctctggtg tcatccggta gcggccactg
     2461 tctgccaggt attcgacata gcccgatgcc gcctgcgcat tcagccattc ccgcacatag
     2521 cgctcatggg tttccgtgcg ctctgccagc tgggccgacg tcagggcacc gccttcggcc
     2581 agcgcccggt acagccccag ctggtcaccg atcaggacca gcggcgccat cgacacggca
     2641 ccaaaatcga cgacgatgcg ccccagcaat gcattcattt tgtcttcatt gataacgctg
     2701 ttcatctcaa gtcctccggg ttgaaagtga acacagtatc ggtgcatgat gatttggccg
     2761 ctttgcagcg cggccatcat tcttgcttgc gcaaccgcag gattgatcgg acttgaccgg
     2821 ccggccggaa tcattgaccg gcagaccact acgccagatc gaaaccggac atggatgcac
     2881 tgtcagaact gcttcgcgtc atcaaactca gtggcgccat ctttttcagg gcggattatt
     2941 cggcgccctg gtgcgtgcgt tcgccggcgg ccgagcgatt cagggaatat gttgccacac
     3001 cgtccaatcg cgtcatcgaa tttcacctga tcgccgacgg gcgcggttat gtgcgggtag
     3061 gcgaagagac aacccctctg gcggcggggg atttcattat gattccccac ggcgacgggc
     3121 actacatggg caacggcagc ggcgtgaagc cggtcgatgg cgaagcctcg ctgccattgc
     3181 tgctgaacca gaacctggaa attgccgaca tgggtggcgg tggcgaacgc acccggctga
     3241 tctgcggcta tctggtatgc gaggaccgcc tgatccagcc gctgctgacc ggattgccgc
     3301 gcgtggtgcg cgtgaacatg cgcgcagatc ccgccagcga attgctggaa ggcatgatcc
     3361 gccatgcggt ggaacaggcg gcagcgtccg cgcccggcag ccgggtgatt gcggcgcggc
     3421 tggcggaagt gctgtttgtg gaagtgttgc ggcgctatct cacccagttg ccaccggggc
     3481 agaccggctg gctggcgggt gcggcagatc cggcgatcgg tcgcgccctg gcagccctgc
     3541 atcagcggcc agaataccgc tggagcctgg acgcattggc gcaggaggcg aacctgtcgc
     3601 gctcggcgct gacagaacgc ttctcccgtt atttcgatga cggaccgatg gcgtatctgg
     3661 cgcagtggcg gctggagctg gcggccgaat cgctgcgtaa caccagccgc agcgtgctgc
     3721 agattgccag tgaagtcggc tacgattccg aagcggcgtt caaccgggca ttcaaacgcc
     3781 gtttttctgc gccaccagga cagtaccgga aagcctggcg ggaaaacgcc gccggaggcc
     3841 ccgcagcgct ggagcgtgcg ctgcagaact gacaggagcc ccttctggca agcactcgcc
     3901 ttacaggggg cttctgcgca agcttagccg cccgccgcaa cccgcttctc ctccgcccgt
     3961 tttttcttct tcatttcaat cttgtccagt tcctgcaggg tcaccagacc cggcccgtgg
     4021 aaacccagca tcaccagata ggccacaggg ttgtagcgca gcgcacgcgg caccaattgc
     4081 gtatatcgct gcaggcgccg gcacagtttc tggtaacgag cctcgtcatg ggcgctccag
     4141 ggcagattga agcgttcgcg atacagcgga tgcaggcaac gggtcgcaca cagccgcagc
     4201 gtgggataaa cgtagcgctc atggaacgca ttcgccagcg gctgcagacc cttgaaatat
     4261 ttcagccgcg cgggcgattc gat
//
LOCUS       DASUEG010000734         8126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_183313, whole genome shotgun sequence.
ACCESSION   DASUEG010000734 DASUEG010000000
VERSION     DASUEG010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183313"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..102)
                     /locus_tag="VFX11_13825"
     tRNA            complement(26..102)
                     /locus_tag="VFX11_13825"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Asp,seq:gtc)
     gene            complement(118..193)
                     /locus_tag="VFX11_13830"
     tRNA            complement(118..193)
                     /locus_tag="VFX11_13830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Val,seq:tac)
     gene            complement(344..820)
                     /locus_tag="VFX11_13835"
     CDS             complement(344..820)
                     /locus_tag="VFX11_13835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX4939752.1"
                     /translation="MKHLHVSIADQTLTLLEDGQPIAHYLVSTALKGPGECSGSEQTP
                     RGHHIIRAKIGAGAPFGAVFRGRRPTGEIWTPELHDEHPGRDWILSRILWLSGTEPGR
                     NRLGRCDSMRRYIYIHGTPDSEPMGVPLSHGCIRMRNVDVIDLFDRIPAYTPVFIE"
     gene            complement(820..1461)
                     /locus_tag="VFX11_13840"
     CDS             complement(820..1461)
                     /locus_tag="VFX11_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4939753.1"
                     /translation="MPEAWRVLRIQSELVDGIEHLTKIAHAAIVFGSARLGPDSPYYH
                     MAEELGSLLSHAGLPVISGGGPGIMEGVNKGAFGKGAQSIGLNINLPREQEANPYQDI
                     SLYFRYFFVRKFMFMKHAVAFAIFPGGYGTLDELFEALTLIQTEKTEPFPVVLIGSSY
                     WKGMVDWLKDTMLMQGCISPEDMELFTVVDSVDEAARIIIAHYRKHYAADKDS"
     gene            complement(1548..1802)
                     /locus_tag="VFX11_13845"
     CDS             complement(1548..1802)
                     /locus_tag="VFX11_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939754.1"
                     /translation="MSAAAFLEIVETEEGDVILRRADSMEGSSEPLVAIRFSPEARAM
                     LGTHLSEIARGMIGAGVQLAGQLQATQGMVVEDEGPHLLH"
     gene            complement(1862..4495)
                     /gene="topA"
                     /locus_tag="VFX11_13850"
     CDS             complement(1862..4495)
                     /gene="topA"
                     /locus_tag="VFX11_13850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX4939755.1"
                     /translation="MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPVSGSGK
                     PVDAAERAKAAAATRKLSAEAKALQKETKSRAALVARMGVDPEKDWKAHYEILPGKEK
                     VVDELKKLAKEADTVYLATDLDREGEAIAWHLREVIGGDDSRFRRVVFNEITKNAITE
                     AFKQPGVLNIDRVNAQQARRFLDRVVGYMVSPLLWEKIARGLSAGRVQSVAVRLVVER
                     EREIRAFIPEEFWEIHADTQTAKKAALRLEVTKQKEAAFRPKNKVEADAALKALNGAS
                     YVVAKRDDKPTRTRPNAPFITSTLQQAASTRLGFSVKKTMTLAQRLYEAGHITYMRTD
                     STNLSQDALSAVRGFIEDQYGKKYLPEKPNLYSSKEGAQEAHEAIRPSDVNATPGSLH
                     GLERDAERLYELIWRQFVACQMPDAEYLSTSLSVNAADFELRTRGRILKFDGFTKVMA
                     PAQKDKEDVVLPDVAVGETLALVKLDPSQHFTKPPARYSEATLVRELEKRGIGRPSTY
                     AAIISTIQERGYVKLENRRLYAEKMGDIVTDRLTENFQNLMDYAFTAELEQELDKVAE
                     GSLEWKKVLNDFYKGFQKRLQKAGQEDGMRRNQPVTTNIPCQLCGRPMQIRTASTGVF
                     LGCSGYALPPKERCKGTINLVPGGEVVAIDENDEESESRQLLARHRCPKCGTAMESYL
                     IDEGRKLHVCGNNPDCDGYEIEQGSYRIKGYDGPLIECDKCGSDMQLKTGRFGKYFGC
                     TNSACGNTRKLLRNGEAAPPKADPIPMPHLRCEKSDDFFLLRDGAAGLFLAASQFPKH
                     RETRAPLIEELKSVADKLDPKHRYLLQAPVQDDEGNKSMLRFSRKTKEQYVVTEKDGK
                     PTGWQAFYQDGKWVVSPAKGK"
     gene            complement(4617..4973)
                     /locus_tag="VFX11_13855"
     CDS             complement(4617..4973)
                     /locus_tag="VFX11_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939756.1"
                     /translation="MALRTEARRLGLHARLLAPPDWYRGEKPRGGMLACYSVLTGDED
                     MGLPYFLAERLSDGEWVVRKGDKLALPSLPSASNAWVALEARANTLSIWWHEEGTPDD
                     LPVQLEVLGKLRQRLS"
     gene            complement(5063..5800)
                     /locus_tag="VFX11_13860"
     CDS             complement(5063..5800)
                     /locus_tag="VFX11_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939757.1"
                     /translation="MTVPRSENIMTTSIQYYFTFDDGLELKFNVDLDRVFDPKRIPVD
                     APEWTRLGNNQCSNCPLNKAEYSHCPAALDLDKMARDFQKLPAHTKANVRVVTPEREY
                     VKRVGLEEGVRALMGLIMATSACPIFGALKANARNHLPFATREEFILRSASMYLMRQY
                     FVMREGGTPDWELKGLVKTNEQLQLVNHAFWQRSVTAFQNDANSKALLSFFTMSSSVS
                     ASLDAQLGKIKPMFFSNGEVKKEAVTP"
     gene            complement(5964..6767)
                     /locus_tag="VFX11_13865"
     CDS             complement(5964..6767)
                     /locus_tag="VFX11_13865"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX4939758.1"
                     /translation="MHVGALAIYPLKSGRQVLVGSMSLDRFGPAGDRRWMLVDDAGRF
                     VTQRTHPRMCLLSAVESVEGLRLQAPGMPDIHVLRPVEGGAAVVSVWEDQVDARLAGP
                     DADAWCSRFLGMPVRLVWMPESTYREVSPAHAGEGHTTGFSDGFPLLLATQSSLDLLN
                     SQLQEPVDWRRFRPNIVVAGDLAPHAEDGWKRLRIGEVELAVVKPCSRCVIPSINPDT
                     ADKNGQILTVMRGYRAAADGKTYFGQNVIVVTRPEVAVIRVGDPVELLA"
     gene            6926..7531
                     /gene="lexA"
                     /locus_tag="VFX11_13870"
     CDS             6926..7531
                     /gene="lexA"
                     /locus_tag="VFX11_13870"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX4939759.1"
                     /translation="MEGLTTRQAEILDFIRENIEHTGMAPTRAEVALRFGFKSKNAAA
                     DHLRALEQKGYIRLHPELSRGIQLLDEQDSDLIPVIGSVAAGLPIEAIENHDEALPVP
                     KGLFAKRPTYLLRVKGDSMKDAGILEGDLIAVYKTNIARKGQIVVARMENEVTVKYLD
                     SEGTQMKLVPANPSYQPMLIAPEDLVIEGVFVGLIRDSFGY"
     gene            complement(7599..>8126)
                     /locus_tag="VFX11_13875"
     CDS             complement(7599..>8126)
                     /locus_tag="VFX11_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX4939760.1"
                     /translation="GIGKPFMDYMTNLKKLAGMVTLIHDHNVGEIRWDEGSKRIDYRI
                     AESDFPAMKASIKAAAKVHFAAGAKRVFVPTVQQKVIGNVNEIDAVVDEIELSPQAFR
                     MVSYHPQGTCRMGMDAKKSVVNPHGETHDVKGLFITDASLFPTSIIVNPQVTVYALSS
                     YITDHIVQNQATYFA"
BASE COUNT         1700 a         2602 c         2178 g         1646 t
ORIGIN      
        1 tttcggacag cgcgtctgtt tgatatggcg cagcggacgg gactcgaacc cgcgaccccc
       61 ggcgtgacag gccggtattc taaccgactg aactaccgct gctcggtctt gacgttgtgg
      121 tgggtgctga cgggatcgaa ccgccgaccc gctccttgta agggagctgc tctcccagct
      181 gagctaagca ccctcagcca tgcaacgtgg ggcgcattat aggggtttgg attttcctgt
      241 caaagggtaa atgcggtttt ttttcatata tttttcaatt gcttattttt cagtcaaccc
      301 gttcccgccc gtccccgctc cagcaggcgg aatccggccg ggcttactcg atgaacaccg
      361 gtgtatatgc aggtatacgg tcaaacaggt caatcacgtc cacattccgc atgcggatac
      421 aaccatgtga cagcggcacc cccatgggct cggagtccgg cgtcccgtga atgtagatgt
      481 agcgtcgcat cgagtcgcac cggcccaaac ggttcctgcc tggctccgta ccagacagcc
      541 agaggatacg actcagaatc cagtctcgcc cggggtgctc atcatgcagc tccggcgtcc
      601 atatctcccc ggtcggacga cgcccgcgaa agacggcccc gaagggagct cccgcaccga
      661 tcttggcgcg gatgatgtga tggccacgag gagtctgctc gctgccactg cactcccccg
      721 gccccttgag cgccgtcgac accagatagt gggcaatcgg ctgaccatcc tccagcaacg
      781 tcaacgtctg gtcagcaatc gacacgtgca gatgcttcat cagctgtcct tgtcagcagc
      841 gtagtgtttg cggtagtgcg cgatgatgat acgggcggcc tcatccacgg agtccaccac
      901 agtaaacagc tccatgtctt ccggcgaaat gcagccctgc atcagcatgg tatccttcag
      961 ccaatccacc attcctttcc agtagctgct cccaatcagc accaccggaa atggctcggt
     1021 cttttccgtc tggatgaggg tcagcgcctc aaacagctca tccagcgtac cgtagccacc
     1081 cggaaaaatg gcaaaggcta ccgcatgctt catgaacatg aacttgcgca cgaagaaata
     1141 gcggaagtag agactgatgt cctggtaggg attggcttcc tgctcacgcg gcaggttgat
     1201 gttgagcccg attgactgcg cccccttgcc aaacgcgccc ttgttgacgc cctccatgat
     1261 gcccggtccg ccaccactga taacaggcag cccggcatgc gacagcaggc tgccgagttc
     1321 ctcggccatg tggtaataag gagaatcagg gcccaatcgg gcgcttccaa agacaatcgc
     1381 cgcatgggca atcttggtga gatgctcgat cccgtcaacc agctctgatt gaatacgcag
     1441 cacccgccag gcttccggca ttttcctgtc gaacatggca gactccgttg tctttttcag
     1501 gagtctagca gcgccaggat gaccacgcga tgcacagcca ccacccatca gtgcaacagg
     1561 tgcggcccct catcctctac aaccatgccc tgggtggcct gaagctggcc ggcaagttgc
     1621 accccggcac cgatcatgcc ccgggcaatc tcgctcaggt gcgttcccag catagccctg
     1681 gcctccggag aaaaccggat agccacgagc ggctctgacg agccttccat cgaatcggca
     1741 cgacgcagga tcacatcgcc ctcttcggtt tccacaattt caagaaacgc agcagcagac
     1801 atctttcttc cacattttct cctgccggta ctggcaggag atctctgatc acatgaaggt
     1861 gttacttgcc cttggccgga ctgaccaccc actttccgtc ctggtagaag gcctgccagc
     1921 cggtcggctt gccgtccttc tccgtgacca catactgctc cttcgtcttg cgactgaagc
     1981 gcagcatgct cttgttacct tcgtcatcct gaaccggtgc ctgcaacagg taacgatgct
     2041 tcggatccag cttgtccgcc acactcttca gctcttcgat cagaggcgcc cgcgtctcgc
     2101 gatgcttggg gaactgactc gctgccagga acagaccggc cgcaccgtca cgcagcagga
     2161 agaagtcatc cgacttctcg caacgcaggt gtggcatcgg gatcggatcc gccttcggcg
     2221 gtgccgcctc accgttgcgc agcagcttgc gggtattgcc gcaggccgag ttcgtgcaac
     2281 cgaaatactt gccgaagcga cccgttttca actgcatgtc cgagccgcac ttgtcacatt
     2341 cgatcagcgg accgtcatag cccttgatgc gatagctgcc ctgttcgatc tcgtagccgt
     2401 cacaatccgg attattaccg cagacatgca acttgcgacc ttcatcgatc aggtagctct
     2461 ccatcgccgt tccgcacttc gggcaacgat gacgcgccag caactggcgg gattcgcttt
     2521 cctcgtcatt ctcgtcaatc gcgacgacct caccgcccgg caccagattg atggtgccct
     2581 tgcaacgctc cttgggcggc aaggcataac ctgaacaccc aaggaacaca cctgtgctgg
     2641 cggtgcgaat ctgcatcggg cgaccgcaca gctggcaggg aatattggtg gtaacaggct
     2701 ggttgcggcg catgccgtct tcctgcccgg ccttctgcag gcgcttctgg aagcccttgt
     2761 agaagtcgtt caggaccttc ttccactcca gcgagccctc agcaaccttg tcgagctcct
     2821 gctccagctc ggccgtaaag gcgtagtcca tcaggttctg gaagttttca gtaagacggt
     2881 cggtaacgat atcgcccatc ttctccgcat agagacgacg attctccagc ttgacgtagc
     2941 cgcgctcctg aatcgtcgag atgatggcgg cataggtgga cggacgacca atgccgcgct
     3001 tttccagttc gcgcaccagc gtggcttcgc tgtagcgggc cggcggcttg gtgaaatgct
     3061 ggctcggatc cagcttcacc agcgccagcg tctcgcccac ggcgacatcc ggcagcacca
     3121 cgtcttcctt gtccttctgc gccggcgcca tcaccttggt gaagccgtca aacttcagga
     3181 tgcggccgcg ggtacgcagc tcgaaatcag ccgcattcac actcaggctg gtcgacaggt
     3241 attcggcatc cggcatctgg cacgccacga actggcgcca gatcaactcg tacaaacgct
     3301 cggcatcccg ctcaaggcca tgcaggctgc ccggcgtggc attcacgtcg gagggacgaa
     3361 tggcttcatg cgcttcctgc gcaccctcct tgctggagta gagattcggc ttctcaggca
     3421 ggtacttctt gccgtactgg tcttcgatga aaccgcgcac cgcactcagc gcatcctggc
     3481 tcagattagt cgagtcggta cgcatgtagg tgatatggcc ggcttcgtac aaacgctgcg
     3541 ccagcgtcat ggttttcttg acggaaaacc cgagacgggt gctggcggcc tgctgcagtg
     3601 tcgacgtaat gaagggcgca ttcggacgcg tgcgtgtcgg cttgtcgtcg cgcttggcca
     3661 cgacatagct ggcaccattc agcgccttca gcgcggcatc tgcttccacc ttgttcttcg
     3721 ggcggaacgc cgcctccttc tgcttcgtca cttccagacg caacgccgcc ttcttggccg
     3781 tttgcgtatc ggcgtgaatt tcccagaact cttcgggaat gaaggcgcga atctctcgct
     3841 cacgctccac caccagacgc accgccaccg actgcactcg tccggcggac aaaccgcggg
     3901 caatcttttc ccagagcagc ggcgacacca tgtaacccac gacacggtcg aggaaacggc
     3961 gcgcctgctg ggcattgacg cgatcaatgt tcaatacgcc aggctgcttg aacgcctcgg
     4021 taatcgcatt cttggtgatt tcgttgaaca ccacgcgacg gaaacggctg tcatcgccgc
     4081 caatcacctc gcgcagatgc caggcgatgg cctccccttc gcggtccaag tcggtggcca
     4141 ggtaaaccgt gtcggcctcc ttcgcgagct tcttcagctc atccaccacc ttttccttgc
     4201 cgggcaggat ttcgtaatgc gctttccagt ccttctccgg gtccaccccc atgcgggcca
     4261 ccagggcagc acgggacttg gtttccttct gcagagcctt ggcctcggcc gagagcttgc
     4321 gggtcgccgc ggcagccttg gcccgttcag ctgcatccac gggcttgccg gaaccgctga
     4381 caggcaggtc gcgcacatgg cccacgctgg atttcacgat gaaatccttg ccgaggtact
     4441 tgttgatggt cttggccttg gccggagact cgacgatgac gagcgacttg cccatgaaat
     4501 cggctacccg tgggaatatg gattagcggc taacgaagcc ggagagcggc atatataaga
     4561 ggggaatgca tcccggtcaa gcgaatagcc tgccggaccg ggtatgacca aggcgatcag
     4621 gagagccgct gacgcagttt tcccaacact tccagctgaa ccggcaggtc gtcaggagtc
     4681 ccttcctcgt gccaccagat gctcagtgta ttggcccggg cctccagtgc cacccaggca
     4741 tttgaggcgg aaggcagcga aggcaaagcc aacttgtccc ccttccgcac cacccactct
     4801 ccatccgaca gacgctcggc caggaagtag ggcaagccca tgtcctcatc ccccgtcagc
     4861 acggaatagc aggccagcat gccccctcgc ggcttttcgc cacgatacca gtcgggagga
     4921 gccaggagac gggcatgcag acccaggcga cgtgcctcgg tccgcaacgc catgagacgc
     4981 tggtcgcggg gactgggacg cagcagggtc atttgccccc aaacgaacgc caccaccagg
     5041 aggacagcaa tcaggaatcc agtcacgggg tgacggcttc cttcttcact tcgccattgg
     5101 aaaagaacat cggcttgatc ttgccaagct gggcatccag cgaagcagac acgctcgacg
     5161 acatggtaaa gaagctcaac agcgccttgg aattggcatc attctggaag gctgtcacgc
     5221 tacgctgcca gaaagcatgg ttcaccaatt gcaattgctc gttggtcttg accagcccct
     5281 tgagctccca gtccggcgtc ccgccctcac gcatcacgaa atactggcgc atcaggtaca
     5341 tcgaggccga acgcagaatg aactcttcac gagtggcaaa cggcagatga ttacgggcat
     5401 tggccttgag cgcaccaaaa atggggcagg cgctggtggc catgatcagg cccatgaggg
     5461 cacgcacacc ctcttccagc ccgacacgct tcacgtattc gcgctccggg gtgactaccc
     5521 tcacgttcgc ctttgtatgg gccggcagtt tctggaaatc acgggccatc ttgtcgagat
     5581 ccagcgcggc gggacaatgg ctgtactccg ccttgttcag cggacagttg ctgcactggt
     5641 tgttgcccag acgcgtccac tcgggcgcgt caaccggaat ccgcttggga tcaaaaacgc
     5701 ggtccaaatc cacgttgaac ttgagttcaa ggccgtcgtc gaaagtgaaa tagtattgaa
     5761 tgcttgttgt catgatgttc tctgaccgcg gcacggtcat ggtatttcct tggccggaac
     5821 taggcagccc tgcaaatggc ggtaggcaaa gccaaaccgg cccctacctt aatccatatg
     5881 caaaccccaa gccagtggcg gatgcaaagc ccgtcggcta tccagaactg cacgctgcgt
     5941 cacaaaccat cccgcctggg gtctcaggcc agcaactcca ctggatctcc cacacgaatg
     6001 acagccacct ccggacgtgt gacgacaatc acattctggc caaagtaggt tttgccatcg
     6061 gccgcagcgc gatagccacg cattactgtc agaatctgac cgttcttgtc agctgtatcc
     6121 ggattgatgg agggaatgac acatcgcgaa cacggtttga ccacggccag ctccacctca
     6181 ccgatccgca accgtttcca gccatcctcc gcatgaggcg ccaaatcacc tgccaccacg
     6241 atattgggac ggaaccgtcg ccagtcgacc ggctcctgca actgtgaatt caggaggtcc
     6301 agtgaggact gtgtcgcgag caaaagcgga aagccatccg aaaagccggt cgtatgcccc
     6361 tcccctgcat gtgcagggct gacctcgcga taagtacttt ccggcatcca gaccagacgg
     6421 acaggcatac ccagaaaccg gctgcaccag gcatcagcat cgggacctgc cagcctggca
     6481 tcgacctgat cctcccagac cgataccacc gcagctccac cttctaccgg ccgcaggaca
     6541 tgaatatccg gcatgcccgg ggcctgcagc cgaagcccct ctactgactc aaccgccgac
     6601 agcaggcaca tgcgcggatg cgtacgctga gtaacaaagc ggccagcatc atcaaccagc
     6661 atccagcggc ggtcgcctgc cggcccgaaa cggtcaagcg acatggaccc caccagcacc
     6721 tgacgccccg atttcaatgg ataaatggcc agtgcgccaa catgcatcga aagtcactcc
     6781 cttgccaaaa ggccgattct actgccattc gtcgccagat gatcccgcca cctactgaca
     6841 taaaagacag gactgttcaa aaggtcagta ccctgataat gtcaggctat caaatcccga
     6901 tggtcgggca gacaggagtt ttgcaatgga aggtttgacc actcgccagg cagaaattct
     6961 cgactttatc cgtgagaaca tcgagcacac cggcatggcc cccacccgcg ccgaagtcgc
     7021 cctgcgcttc ggcttcaagt ccaagaacgc tgctgccgac catctgcgcg ccctggaaca
     7081 gaagggctat atcaggctgc acccggagct gtcccgcggc atccagctac tggacgagca
     7141 ggactccgac ctgatcccgg tcatcggctc tgttgctgcc ggcctgccca tcgaggccat
     7201 cgagaaccac gatgaagcac tgccggtgcc gaaaggcctc ttcgccaagc gccccaccta
     7261 cctgctgcgg gtaaagggcg actccatgaa ggatgccggc attctcgaag gcgacctgat
     7321 tgccgtgtac aagaccaaca ttgcccgcaa ggggcagatt gtcgtggccc gcatggaaaa
     7381 cgaagtcacg gtgaaatatc tggactccga aggcacccag atgaagctgg tacctgccaa
     7441 tcccagctat cagcccatgc tgattgcgcc ggaagacctg gtcatcgaag gcgtgtttgt
     7501 cggactgatc cgtgactcct tcggttactg atacccctct cacgcaataa aaaagccggc
     7561 attcctgccg gctttttcat tcagctccgc acacccgctc aggcgaaata cgttgcctga
     7621 ttctgtacga tgtggtcggt gatatagctc gacagtgcat acaccgtgac ctgcggattc
     7681 acgatgatgg atgtcggaaa aaggctggca tccgtgatga aaagcccctt cacgtcatgc
     7741 gtttcaccat gggggtttac caccgatttc ttcgcatcca tccccatgcg gcatgtgccc
     7801 tgagggtggt aggacaccat gcgaaaggct tgtggagaca actcgatctc gtccaccacc
     7861 gcatcaatct cattcacatt cccgatgact ttctgctgca cggtcggcac gaataccctt
     7921 ttggcacctg ccgcaaaatg cacttttgcc gccgccttga tagacgcctt catggcagga
     7981 aaatccgact ctgcaatccg gtagtcaatg cgcttgctac cttcatccca gcggatctca
     8041 cccacattgt ggtcatgaat cagcgtcacc ataccggcca gttttttcag gttcgtcatg
     8101 tagtccatga agggtttgcc aatacc
//
LOCUS       DASUEG010000735         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2107378, whole genome shotgun sequence.
ACCESSION   DASUEG010000735 DASUEG010000000
VERSION     DASUEG010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107378"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFX11_13880"
     CDS             <1..298
                     /locus_tag="VFX11_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX4939761.1"
                     /translation="ALFDTDAGPMAVILVGAMVVASIETVWAGLVTPPKRQLKVTDYS
                     PRPQPITLERGAEMGRFKLGSTAIVLFGPDVVKWKDGLQEGSPTAMGEQIATLA"
     gene            complement(389..715)
                     /locus_tag="VFX11_13885"
     CDS             complement(389..715)
                     /locus_tag="VFX11_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939762.1"
                     /translation="MELTEQLLGDCSPYISNLVYDIDVRMLFIECFDDPEAQNPLLRI
                     VFPGILSYAETSLITEPEDETVDDIVSIERLDARKIVITTYKKEILLTLTEEPFVEDI
                     EGEDND"
     gene            complement(788..1864)
                     /locus_tag="VFX11_13890"
     CDS             complement(788..1864)
                     /locus_tag="VFX11_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939763.1"
                     /translation="MEEARKLRTILYPLARDIRNLSTFVANINNVLQADAQRFAIGAP
                     VGLLSIRNSVRSLNKNTRAMKEVNDIALQESSVAASLAEATGNTLLRPALQLNLLARN
                     AKPQVDRTYSCVLRIEGYLNPVFVFMAALIPVVQSMQSDVQALDDRMAQFKKAVSRLA
                     DQELTSGLPGEAEQQLEQLIPQFSVIEQETSDISAQVGLLMGKMNRLSELSSKLEILL
                     RMSAALDGSLNELLPAMAVLKNLSVVLNQAQRQHEPQGQSLEARVAEAVAGLKLPSDM
                     LLQLETRLAQQVDRYINPIAQPLQELAEQLRPRMPSTHELNGLESTLVAQSNRFGNVN
                     KLLTRLFSNLDRVIAQVNRTTHAA"
     gene            complement(2102..2698)
                     /locus_tag="VFX11_13895"
     CDS             complement(2102..2698)
                     /locus_tag="VFX11_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4939764.1"
                     /translation="MRLYQYHPSGNCYKIRLLLNQLGIPYEKVEIDILRRESRTPEFL
                     RINPNGRIPVLDVNGRYLPESNAALYYLAQGTRFFPQDRFAQAQVLQWMFFEQYSHEP
                     YIATIRFWVAFQRSAQKNADEIRRRMPLGYAALEVMERHLSQNLYFVDNRYSIADITL
                     FAYTHVAEEGGFQLSRYPHLQAWIRRVQQQPGHISIED"
     gene            complement(2722..3021)
                     /locus_tag="VFX11_13900"
     CDS             complement(2722..3021)
                     /locus_tag="VFX11_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX4939765.1"
                     /translation="MLRRVVLWRLRATEPEQKALDAGTIKRALESMRGKIPGMINIQV
                     GINTAQMADASDVVMIVDFADEQALAVYEKHPVHEQVKPIVGPLRDERRVVEYHC"
BASE COUNT          664 a          931 c          967 g          701 t
ORIGIN      
        1 ggcgctgttc gacaccgacg caggcccgat ggcggtgatt ctggtgggcg cgatggtggt
       61 ggccagcatc gaaaccgtgt gggccggcct ggtgacaccg ccgaagcggc aactgaaagt
      121 gacggattac agcccgcgtc cgcagcccat cacgctggaa cgcggcgccg agatgggccg
      181 cttcaagctg ggctccaccg ccatcgtgct gtttggtccg gatgtggtga agtggaagga
      241 tggcctgcag gaaggctcac ccactgcaat gggcgaacag attgcaacgc tggcctgaag
      301 ctaaaaccgc ggacgctgaa acaggcgggc actgaattct cagcgccggc cctccgcacg
      361 gtggatcagg cggcggccgg cagacaactc aatcgttgtc ttccccttcg atgtcctcga
      421 caaacggttc ttccgtcagc gtcagcagga tttccttctt gtacgtggtg atcacgatct
      481 tgcgggcatc cagacgttcg atgctgacaa tatcgtccac cgtctcatct tccggctcgg
      541 tgatcaggct ggtctcggca taggacagaa tgccggggaa aacgatgcgc aacagcggat
      601 tctgcgcttc ggggtcatcg aagcattcga tgaacagcat gcgcacatcg atgtcgtaaa
      661 ccagattgga gatatagggc gagcaatcgc ccagcagctg ttcggtcagt tccatggcgc
      721 accggagttg tcgttagcgg tgcgccattg taagccgaca gcctgcggga aagaaggaaa
      781 agaagggtca ggcggcgtga gtcgtccgat tcacctgggc aatcacccga tccaggttgc
      841 tgaatagccg cgtcagcagt ttgttgacat tgccgaagcg gttgctctgc gccaccagcg
      901 tactttccag gccattcagt tcgtgagtag acggcatgcg gggccgcaac tgctcggcca
      961 gttcctgcaa cggctgcgcg ataggattga tgtagcgatc cacctgctgg gccagacggg
     1021 tttccagttg cagcaacatg tctgatggca gtttcagccc ggccactgct tccgccaccc
     1081 gggcctccag cgactgaccc tggggttcgt gctgccgctg cgcctggttc agcaccacac
     1141 tcagattttt cagcaccgcc atcgccggca gcagttcatt gagggaacca tccagcgccg
     1201 cactcatgcg caacaggatt tccagcttgc tgctgagttc ggacaagcgg ttcatcttgc
     1261 ccatcagcag gcccacctgg gcagaaatgt ctgaggtttc ctgttcgatc acggaaaact
     1321 gtggaatcag ctgctccagc tgttgttccg cctcacccgg caggcccgag gtcagttcct
     1381 ggtccgccag ccgcgacacg gccttcttga attgcgccat gcgatcgtcc agtgcctgca
     1441 cgtcggactg catcgactgc accaccggaa tcaacgcagc catgaaaaca aatacggggt
     1501 tgagataacc ctcgatgcgc agtacacagg aataggtgcg gtccacctgc ggcttggcgt
     1561 tacgcgccag caggttgagc tgcagcgccg gacgcaacag cgtgttgcct gttgcttccg
     1621 ccagcgacgc cgccacactg ctttcctgca aggcgatgtc gttgacttcc ttcatcgccc
     1681 gggtgttttt gttgaggctg cgcacgctgt tgcggatgct gagcaaaccc accggcgcgc
     1741 caatggcgaa acgctgggca tcggcctgca gcacattgtt gatgttggca acgaacgtgg
     1801 acagattgcg gatatcacgg gcgagaggat acagaatggt cctgagctta cgagcttctt
     1861 ccatgggtct ctccttgctg agaaagggtg aacgcttcac gaggctgcgc acacaacggc
     1921 attcaattgc cagatattcg ttcgctgtgt tggttttcgc ttgcacaggc acaaaagtca
     1981 cccgcctgca caacagtcca cgctgaaaaa gagtatagcg gcaggcataa cgccctgcag
     2041 agacctgcca gacaaaaatt ttggtatttt atcaggcaat caattttagg gcgttttttg
     2101 atcaatcttc gatggaaatg tgccccggtt gctgctgcac ccgccggatc caggcttgca
     2161 ggtgggggta gcggctaagc tggaaaccgc cttcttcagc cacgtgggtg taggcgaaca
     2221 gggtgatgtc ggcgatgctg tagcggttgt cgacgaaata cagattttgc gacaggtgcc
     2281 gctccatgac ctccagcgcg gcataaccca gcggcatgcg gcggcggatt tcatcggcat
     2341 ttttctgcgc cgagcgctgg aacgccaccc agaaacggat ggtggcgatg tagggttcat
     2401 ggctgtattg ctcgaagaac atccattgca gcacctgggc ctgggcaaac cggtcctgcg
     2461 ggaaaaagcg ggtgccctgg gccaagtaat agagcgcagc gttggattcc ggcagataac
     2521 gcccattcac gtccagcacg ggaatgcgac cgttgggatt gatgcgcaga aattccggcg
     2581 tgcgggattc gcgcctgaga atgtcgattt caactttttc gtagggaatg cccagctggt
     2641 tcagcagcag gcggatcttg tagcagttgc cggaggggtg gtactggtaa agacgcatgg
     2701 ggaaggctcc tttttccgtt ttcagcaatg gtattccacc acccgccgct catcgcgcaa
     2761 cggtcccacg atgggtttca cctgttcgtg caccgggtgt ttttcataaa ccgccagcgc
     2821 ctgctcatcg gcgaaatcca cgatcatcac cacgtcggag gcatcggcca tctgcgcggt
     2881 attgataccg acctggatat tgatcatccc gggaatcttg ccgcgcatgg attccagcgc
     2941 acgcttgatg gtgccggcgt cgagggcttt ctgttcaggt tcggtggcgc gcaggcgcca
     3001 gagcacgact cgtcggagca tgggacattt cctgtttcaa acagtcttgg ttagcagtac
     3061 agaggagtga atatgcggcg catgtaaatt actgatcgac cataaacgcc agcatctgtt
     3121 ccagctgggt ttccagcatc cattttccgc cgtgaatgtt gcagcctttc tcctgcgcga
     3181 tctgcagcag cggcgtcatc tcgttattca ccaccacgtc cgccacccac atatgcggct
     3241 gcaggaattt ttccgacagc ggc
//
LOCUS       DASUEG010000736         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_428548, whole genome shotgun sequence.
ACCESSION   DASUEG010000736 DASUEG010000000
VERSION     DASUEG010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428548"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFX11_13905"
     CDS             complement(<1..1023)
                     /locus_tag="VFX11_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX4939766.1"
                     /translation="MLKRIDGLMWLLVLVLLSRLLAMAGLPLADTSEPRYAEIARVMA
                     QSGDWITPWFEPGRPFWGKPPLAFWSQALAFRALGVSEFAVRFPSWLATLGTLWLLYQ
                     LALNWFDRTVARGSVLILGSMALTFVTAGAVLMDPFLTLGITWSMVAVALVADNPRWY
                     WRYGFFLGLAIGLLAKGPLALVLMVGQLLPWCLLSPQGRAGVRALPWISGGLLLLGLT
                     LPWYVAAELKTPGFLRYFLLGEHILRFIDPGWAGDLYGGAHQRAHGTIWAYWLLAASP
                     WSLLGIALLASRLWRGTVVIRPSQLLRDHRFLFLAGWSLFTPLFFTLAGNILWTYVLP
                     AQPAMAL"
     gene            complement(1007..2026)
                     /locus_tag="VFX11_13910"
     CDS             complement(1007..2026)
                     /locus_tag="VFX11_13910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4939767.1"
                     /translation="MNAPSHLWLPEFSRSAAELLSVVVPVYNEREVLPLFFERLQQAL
                     GRLQMPHEILFVDDGSKDGSVDFLRLLALSVPAVKVIALSRNFGKEAAVTAGFEHAAG
                     DAVILLDADLQDPPELIPDMVAAWRGGADVVCMRRRSRAGETWFKRTSASLYYRLLNR
                     LSRFDIPHDTGDFRLMSRRAVNALNQLPERNRYMKGLFAWVGMPTQVLLYDREPRAAG
                     QTKWQLPALIGLAFEGITSFSTAPLRWTTLAGVVASVWGALFGLWIVMKALLLGDPVA
                     GYPSLIAVLTFLGGVQLLTVGLVGEYVGKIYMEVKQRPVYLVQDVTTMTAVAQKRVAL
                     HAETH"
     gene            complement(1995..2411)
                     /locus_tag="VFX11_13915"
     CDS             complement(1995..2411)
                     /locus_tag="VFX11_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX4939768.1"
                     /translation="MRALPFRHLWLQPARFLASGGLATALHWLVMTALVSIQVPAVAA
                     TALGAIAGACLNYLLQYHFTFRARTDHTKSVPVYVLVAALGWLANLGLFAGLHLLLDL
                     PAASAQVLTTGIVALLNYVLYKRFVFHECAQSPLAT"
     gene            complement(2513..4021)
                     /locus_tag="VFX11_13920"
     CDS             complement(2513..4021)
                     /locus_tag="VFX11_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4939769.1"
                     /translation="MQSPSFPLIPVRDSSATAMVRTSALFVLVALLCLPWADLSISTR
                     DPWQELARLGWGLLTPSFDNPLTLLEALANTLAFALQGVALGAVGGFLLALGYRRAAV
                     RRFAAFIRAIHELFWALLFIQLAGLSPAAGVLAIAIPYAGTFAKIYGELFEEVDPSAR
                     QALPVGTGALSAFFFTELPQCFAHMVTYTRYRLECGIRSSAVLGFIGLPTVGFHLETA
                     LKQGQYSEAAALFYALVLVIVTLHHWMKKKMLPLYLLAVLVWLPPVAQFNGALVWRFF
                     SEDIVPAPLRQGWNGEGLWRWLHALWSNQIAPGLGNTLVLSLLALVGTGLVTLLTFPA
                     ISHLFGRPVTRAGGHFFLVLMRSAPEYLLAFVGLLLWGPSMLPAVVALSLHNGAIIAH
                     LVGHYSDSLQLRDDAVTGVHRYLFEVLPRIYRTFLAFTLYRFEILMRETAMLGILGIP
                     TLGFFIDSAFTEFRFDRAMLLLLVTALLNMSVDMLARTLRRRLHLSSQPESL"
     gene            complement(4026..>4288)
                     /locus_tag="VFX11_13925"
     CDS             complement(4026..>4288)
                     /locus_tag="VFX11_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX4939770.1"
                     /translation="LGRALYQQRPVFLGDEPVSAVDEVQADQLLQLICARHATVVIAL
                     HDVALALRHCNRIIGLQGGRIALDRPASACDPAELLRLYASA"
BASE COUNT         1000 a         1448 c         1261 g          579 t
ORIGIN      
        1 cagggccatg gccggctgcg cgggcagtac gtaggtccac agaatgttgc ccgccagggt
       61 gaaaaacagc ggggtgaaca ggctccatcc tgccaggaaa agaaagcggt gatcgcgcag
      121 cagttgcgac ggccgaatga ccaccgtgcc ccgccacagg cgactcgcca gcagggcaat
      181 gcccagcaac gaccagggtg aagccgccag cagccagtat gcccagatag tgccgtgagc
      241 gcgctggtgc gccccgccgt acaggtcgcc ggcccagccg ggatcgataa accgcagaat
      301 gtgctcgccc agcaggaagt aacgcagaaa gcccggggtt ttcagttccg ccgccacata
      361 ccagggcagt gtcaaaccga gcaacagcag gccgccactg atccagggca acgcgcgcac
      421 gccggcacgc ccttgcggcg acaacaggca ccagggcagc aactgcccca ccatcaacac
      481 cagcgccaga gggcctttcg ccagcaggcc gatcgccagt cccaggaaaa aaccatagcg
      541 ccagtaccag cgcggattgt ccgccaccag ggccaccgcc accatcgacc aggtaattcc
      601 caacgtcagg aacgggtcca tcagcacggc accggccgtc acaaacgtca gcgccatcga
      661 gccgaggatc aggaccgacc cgcgcgcgac ggtcctgtcg aaccagttca gcgccagctg
      721 atacaacagc cacagggtgc caagcgtggc gagccaggag gggaaccgca ccgcgaattc
      781 cgacacgccc agcgcacgaa acgccagcgc ctgactccag aacgccagcg gcggtttgcc
      841 ccagaagggc cggccgggtt cgaaccaggg cgtgatccag tcgccgctct gcgccatcac
      901 ccgcgcgatt tccgcgtaac gtggttccga ggtgtctgcc agcggcaggc cggccatggc
      961 cagcaaccgc gacagcaata ccagcaccag cagccacatc agcccatcaa tgcgtttcag
     1021 catggagtgc cacccgcttc tgggctactg ccgtcatggt cgtcacatcc tgcaccagat
     1081 acaccgggcg ctgcttcacc tccatataaa tcttgccgac atattccccc accagaccca
     1141 ccgtgagcag ctgcacgcca cccagaaacg tcagcacggc aatcagggac ggatagcccg
     1201 ccaccggatc gcccagcagc agggccttca tcacgatcca gaggccgaac agcgcgcccc
     1261 acacagaagc cactaccccc gccagcgtgg tccagcgcag gggcgcggtg gaaaacgacg
     1321 tgatgccttc aaacgccagc ccgatcagcg ccggcaactg ccatttggtc tggccggccg
     1381 cgcggggttc gcggtcgtac agcaatactt gagtcggcat gccgacccag gcaaacaatc
     1441 ctttcatgta gcgattgcgt tcgggcagct ggttcagtgc attcaccgca cgccggctca
     1501 tcagacgaaa atcgccggtg tcgtgcggaa tatcgaaccg gctgagccgg ttcagcaggc
     1561 ggtagtacag gctggcactg gtgcgcttga accaggtttc gcccgcccgg gaacgccgcc
     1621 gcatgcagac aacatccgca cccccgcgcc aggccgccac catgtcggga atcagttccg
     1681 gcgggtcctg cagatccgca tccagcagga tcaccgcatc accggccgca tgttcaaagc
     1741 cggctgtcac ggcggcttcc ttgccaaagt tacggctcag cgcaatcact ttcacggctg
     1801 gaactgaaag cgcaagcaaa cgcaggaaat cgacactgcc atccttgctg ccatcatcca
     1861 cgaacaggat ttcgtgcggc atctgcaacc ggccaagcgc ctgctgcagt cgctcgaaaa
     1921 acagcggcaa tacctcgcgc tcgttgtaaa cgggcaccac caccgacagg agctcggcag
     1981 cgctccgcga gaattcaggt agccaaaggt gactgggcgc attcatgaaa cacaaacctc
     2041 ttgtagagaa cgtagttcaa cagagcgacg atcccggtcg tcagcacttg tgcgctggcg
     2101 gcgggcaggt ccagcagcag gtgcagaccg gcaaacaggc ccagattggc cagccagccc
     2161 agcgcagcaa ccagaacgta gacgggaacg gatttggtgt gatcagtgcg ggcgcggaat
     2221 gtaaagtggt actgcagcag gtagttcagg caggccccgg caatggcacc cagcgcggtg
     2281 gccgcaaccg ccggtacctg aatggaaacc agcgccgtca tcaccaacca gtgcaacgcg
     2341 gtcgccaagc cgccacttgc caggaagcgg gccggctgca accagagatg tcggaagggt
     2401 aacgctcgca atcactgctc ctgttttgag tacaggatca gcttagcgac agaggcaaca
     2461 agaaaggctc aatcaatagt caagggatta tcaaacccct gaccgggggc aattacagac
     2521 tctcgggctg actggacagg tgcaggcgtc gccgcaaggt gcgcgccagc atgtccacgc
     2581 tcatgttcag caacgcagtg accagcagca acagcatcgc gcggtcgaag cggaattcgg
     2641 tgaaggcgga atcgataaag aatcccagcg tgggaatacc caggatgccg agcatggcgg
     2701 tttcccgcat caggatttca aaccggtaca gggtgaacgc gagaaacgtg cgatagatgc
     2761 gcggcagcac ctcgaacaga tagcggtgta ccccggtgac ggcatcgtcg cgcagctgca
     2821 ggctgtcgct gtagtgtccc actaaatgcg caatgatggc gccattgtgc aacgacagcg
     2881 ccaccaccgc cggcagcatg ctcggccccc acagcagcag gccgacaaac gccagcaggt
     2941 attccggcgc ggaacgcatc agcaccagaa aaaaatgccc gccggcgcgc gtcaccggcc
     3001 ggccgaacag atgggaaatg gccgggaagg tcagcaacgt caccagaccg gtgcccacca
     3061 gcgccagcaa cgacaacacc agcgtattgc ccagccccgg cgcaatctgg ttgctccaca
     3121 gggcgtgcag ccaccgccac aacccttcac cattccagcc ctgccgcagc ggcgccggca
     3181 caatatcctc gctgaaaaaa cgccatacca gcgcgccatt gaactgcgct accggcggca
     3241 gccacaccag cacggccagc agatacagcg gcagcatttt tttcttcatc cagtgatgca
     3301 gcgtgacgat caccagcacc agcgcgtaga acaacgcagc ggcttcactg tactgtccct
     3361 gcttgagcgc ggtttccaga tggaatccca ccgtgggcag gccgatgaaa cccagcacgg
     3421 cggaagaacg gatgccgcac tccagccggt agcgcgtgta cgtcaccatg tgcgcaaaac
     3481 actgcggcag ctcggtgaag aaaaacgctg acagcgcgcc agtgccaacg ggcaacgcct
     3541 gacgcgcgga agggtccact tcctcgaaca gctcgccgta aatcttggcg aaagtgcccg
     3601 cgtagggaat cgcgatcgcc agcacgcccg ccgccggcga cagccccgcc agctgaatga
     3661 acagcaaggc ccagaacaat tcgtgaatcg cgcgaatgaa cgcggcaaat cggcgcacgg
     3721 cggcgcgacg ataacccagc gccagcaaaa atccgcccac cgcacccagc gccacgccct
     3781 gcagcgcaaa cgccagcgtg ttggccagcg cttccagcag ggtgagcggg ttatcgaacg
     3841 atggcgtcag caggccccag cccagtcgcg ccagttcctg ccagggatcg cgggtggaaa
     3901 tgctcaaatc cgcccagggc agacacagca gcgccaccag cacgaacagc gcactggtgc
     3961 gcaccatggc agtggcagaa ctgtcgcgca cgggaatcag ggggaaagag ggcgattgca
     4021 ttgcatcacg cagacgcgta cagacgcagc agctctgccg gatcgcacgc actggccggc
     4081 cggtccagcg caatccggcc accctgcagg ccaatgatgc gattgcagtg gcgcaacgcc
     4141 agcgccacat cgtgcagcgc aatcaccacc gtcgcatggc gcgcgcagat cagttgcagc
     4201 aactggtcgg cctgcacttc atccaccgcc gacaccggtt cgtcgccgag aaaaaccggc
     4261 cgctgctggt acagcgcccg gcccaggc
//
LOCUS       DASUEG010000737         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_251784, whole genome shotgun sequence.
ACCESSION   DASUEG010000737 DASUEG010000000
VERSION     DASUEG010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251784"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VFX11_13930"
     CDS             <1..93
                     /locus_tag="VFX11_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4939771.1"
                     /translation="TGKIGDGKVFVQPLEQVIRIRTGETGVEAI"
     gene            139..1641
                     /gene="amt"
                     /locus_tag="VFX11_13935"
     CDS             139..1641
                     /gene="amt"
                     /locus_tag="VFX11_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4939772.1"
                     /translation="MMSVMLGSTLAFAAPVFAEEAPAAAPAAEAVAAEVVAAPEAAAQ
                     AVEAAAPAAAEAAPAEAAPAPTYNKGDNAFLMVCAAFVILMSLPGLALFYGGLVRKKN
                     MLSVLMQVFTVFCLITVLWMVYGYSVAFTEGNAFFGSLSKLFLKGVNDTTLAATWSKA
                     TPISEVIYVVFQGAFAAITCALIVGAFAERAKFAGVLVFSVLWFTFSYLPMAHMVWYW
                     AGPDAYTSAEAATAATATAGWLFQKGALDFAGGTVVHINAAVAGLVGAYVIGKRIGYG
                     REALLPHNMVMTMIGACLLWFGWFGFNAGSALEANGIAGLAFANTWLATAAAALSWSL
                     AEWVLKGKPSMLGAASGAVAGLVAVTPACGFVGLGGALVIGLLAGVICLWGVHGLKKL
                     LGADDALDVFGVHGVGGILGALLTGVFASPSLGGLSWWDYVANAPGAYDMLGQVKVQA
                     ISVVTTVIWSGVVSLVAFKLVDLTIGMRVSEEDERIGLDVTSHGERAYND"
     gene            complement(1737..>2214)
                     /locus_tag="VFX11_13940"
     CDS             complement(1737..>2214)
                     /locus_tag="VFX11_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939773.1"
                     /translation="NANANATASIAPTAATTSVSTSVSMIEGNTGRYLSPYTSDGVAA
                     AWVEKSVNASLGASIGGMAGAYAGQKALEQVPFVGGFLGNKLGAEAGRKIALNAVGGE
                     AYMRETSDISFNDINEMARWLVQNHSSHAKFKEIMKAAGQIYPELNQAYMVALRRR"
BASE COUNT          325 a          652 c          720 g          517 t
ORIGIN      
        1 accggaaaga tcggtgacgg caaggtgttt gtgcaaccgc tggagcaggt gatccgcatt
       61 cgtaccggtg aaaccggcgt cgaagcgatc tgaatccaaa ctggctttta acgcggagga
      121 attcccgtga agaaatttat gatgtctgtg atgctgggta gcacgcttgc gtttgctgca
      181 ccggtatttg ccgaggaggc acctgctgcc gccccggccg ccgaggccgt tgcggccgaa
      241 gtggttgctg ctcccgaagc ggctgcccag gccgttgaag ccgcggctcc tgctgctgcc
      301 gaagctgctc cggctgaagc ggcccctgct cctacctaca acaagggtga caacgccttc
      361 ctgatggtgt gtgctgcctt cgtgatcctg atgtcgctgc cgggtctggc cctgttctat
      421 ggtggcctgg tgcgcaagaa gaacatgctg tccgtgctga tgcaggtgtt cacggtgttc
      481 tgcctgatca ccgtgctctg gatggtgtac ggctacagcg tggccttcac tgaaggcaat
      541 gccttcttcg gctcgctgtc caagctgttc ctgaagggcg ttaacgacac cacgctggcc
      601 gccacctgga gcaaggcaac accgatcagt gaagtcatct acgtggtgtt ccagggcgcc
      661 ttcgcggcga tcacctgtgc gctgatcgtg ggtgcctttg ccgagcgtgc caagtttgcc
      721 ggcgtgctgg tgttctcggt gctgtggttc accttctcct acctgccgat ggcccacatg
      781 gtgtggtact gggcaggtcc ggatgcctat acctctgctg aagccgcgac ggctgccact
      841 gcaacggccg gctggctgtt ccagaagggc gccctcgact tcgccggcgg caccgtggtg
      901 cacatcaacg ccgccgtggc cggcctggtg ggtgcctacg tgatcggcaa gcgtatcggc
      961 tacggccggg aagccctgct gccgcacaac atggtcatga ccatgatcgg tgcctgcctg
     1021 ctgtggttcg gctggttcgg tttcaacgcc ggttccgctc tcgaagccaa cggcattgcc
     1081 ggcctcgctt tcgccaacac ctggctggcc actgccgctg ccgcactgag ctggtcgctg
     1141 gccgagtggg tccttaaggg caagccctcc atgctgggtg ctgcttccgg cgctgtggca
     1201 ggtctcgtgg ctgttacgcc tgcttgcggc ttcgtcggtt tgggcggtgc tctggtgatc
     1261 ggtctgctgg caggtgtgat ctgcctgtgg ggtgtgcatg gcctcaagaa gctgctgggt
     1321 gctgacgacg cgctggacgt gttcggtgtg cacggcgtgg gcggcatcct cggtgccctg
     1381 ctgactggcg tgttcgcttc gccttccctg ggcggcctga gctggtggga ctacgtggcc
     1441 aacgctccgg gtgcctacga catgctgggt caggtcaagg tgcaggccat cagcgtcgtg
     1501 accaccgtga tctggtcggg tgtggtatcg ctggtcgcct tcaagctggt cgatctcacc
     1561 atcggcatgc gtgtcagcga agaagacgag cgtatcggtc tggatgtcac ctcgcatggc
     1621 gagcgtgctt acaacgactg atatcgactg agtcgtgtcg caatgaaaac aggggccttc
     1681 gggcccctgt ttttttgcgc gtccgcgggg ttttgccccg cgctatggtt gctgcttcag
     1741 cgacggcgca gggccaccat gtaggcctga ttcagttccg gatagatctg tccggcggct
     1801 ttcatgattt ctttgaactt ggcgtggctg ctgtggtttt gcaccagcca tctggccatt
     1861 tcgttgatgt cattgaagga aatgtccgag gtctcgcgca tgtaggcttc gccgcccacg
     1921 gcattcaggg cgatcttgcg accagcctcg gcccccagct tgttgcccag gaaaccgccc
     1981 acgaacggta cttgctccag agctttctgg ccggcatagg cgccggccat gccaccgatg
     2041 gatgcaccca gcgaggcgtt gaccgatttt tccacccatg cggcagcaac tccgtcgctg
     2101 gtgtaggggc tcaggtagcg tccggtgttg ccctcgatca tgctgacgct ggttgaaaca
     2161 ctggtcgttg cggcggtcgg cgcgatgctg gcggttgcat ttgcatttgc attt
//
LOCUS       DASUEG010000738         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1850956, whole genome shotgun sequence.
ACCESSION   DASUEG010000738 DASUEG010000000
VERSION     DASUEG010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850956"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..978
                     /gene="tcdA"
                     /locus_tag="VFX11_13945"
     CDS             142..978
                     /gene="tcdA"
                     /locus_tag="VFX11_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HEX4939774.1"
                     /translation="MLPDSYQNRFSGIDRLYGKGALDILRQRHVAVVGIGGVGSWAAE
                     ALARSGVGEITLFDLDDICVSNTNRQIHATTGTFGQMKVDVMAARLKAINPDLIVHAE
                     ADFVTATNVAEKLTKRFHFVLDATDKVKHKTAMIVHCKRNKIPIYVVGGAGGQTDPTR
                     IQYADMTRAHQDPLLALVRKKLRQNHGYSTNLKRRFSVECVFSDEQLMFPQPDGSVCA
                     TRPELDEDSGSIRLDCAGGFGASTCVTATFGFVAVSRIIRKLLDKAWKAPSPSSPDRD
                     ET"
     gene            1047..3032
                     /locus_tag="VFX11_13950"
     CDS             1047..3032
                     /locus_tag="VFX11_13950"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEX4939775.1"
                     /translation="MTSLFVRGSLLTLVVGSLASPVAFAATDLHKFAMIDGISRELVL
                     QENRELMQSLPVENGTAVHYTGQLVDFPDSWARLSRIDGQWQGVVVNSGEIFLVEQLA
                     DSPQEATSRSAEFAVSAVKSFEELGDCAVAHPPAGGSSAPQARALTSQALVGQAVSVN
                     FPAYCSATVNGTCLVAELTVVFDEKFKQAFPSNYTSQAASLLNTLDGFYRQDFKIVFR
                     HLRLDFDNGSTFTTSRDPYDLLADIGEKRFNNSISSFDPNKRSLLHMVTGRDFRFEDN
                     GDINDDVVGLAYSPDYDISNYPNSFYPVLCDSDGSAVGTSQLLYSGNTPSATRTALVV
                     AHEIGHNLGAEHDGTTDSVTASSCTDATKIMNPYVVSNPSGFSTCSQSTIATNISRLP
                     AVEACFDFPVDASLAAVSGNLTQTTPETAFIQQYTAGFSTANGVNGTVTVSGTLNGGD
                     ATFSTVTLAGNGCTLSNGNQTYTCTTGSLASPSALAVSLNANSTHVSIAHSITPSSAN
                     HLYDVDAADNNLSDTILVRDADLPPNSLTATLSGNDVQLAWQDHATNEQGFVVERKKG
                     DGEWTVMAAALATNSVSYTDADVASQSSYTYRVSALFDGERSAPSNEVSIDVSIAPSG
                     TMRIGGSGKGGGGSLSPLLLAFMAGMASLLAFLQRKY"
     gene            3095..4312
                     /locus_tag="VFX11_13955"
     CDS             3095..4312
                     /locus_tag="VFX11_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4939776.1"
                     /translation="MPHSQDATLRRLIDITQSLSSEQDKWRLLERILAESMAITRADG
                     GTLYLMQNDAEGQWLEYGILHNRSLGLVQINRSGSDSALMPIPVYDLHTGSANHQNIA
                     THCALTRRTINISDAYEASQFDISGIRAFDELFDYRTRSMLTLPLQNHAGQVIGVMQL
                     VNATAPDTTGVVPFTDPALSIVQALASLASIILENSLMLEFQRDLLIRLSSAGNTSEL
                     FERILDEALAITRAEGGSLYLVREGNAPRLEFVLLKNQALKLNLGGRHGQPVDMAPLL
                     LEKNGEANLGNIATYTALSRETVNIQNAYHDIRFDFSGMKAFDTDHHYRSRSFLSVPL
                     INHASSVLGVLQLVNARDPDGQIMAFDERQEPIVQALASYAAIALENQQLREDHARLL
                     AEANHHDHRQKPD"
     gene            4688..4912
                     /locus_tag="VFX11_13960"
     CDS             4688..4912
                     /locus_tag="VFX11_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939777.1"
                     /translation="MLASKMNIDLAVHDAQQWTEYEGVSKVMVAANRRSILVVTSCNP
                     AAITAPIPSFFHGFQVQFHQSTQAPQTLES"
     gene            5138..>6053
                     /locus_tag="VFX11_13965"
     CDS             5138..>6053
                     /locus_tag="VFX11_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4939778.1"
                     /translation="MPHARDLEPELKKVSVLFVDITNSVHLIHKLRPEDSTEYLEPTL
                     NALKQSAHRYGGMINRVDGDGMMVIFGAPIAYEDHAVRACYAALDMLKAVKSEFDATH
                     IGVRVGIHSGEVLMKPFYNDFSVDYEALGPTVYLAHRMEVLASPNTAIISRETYRLAQ
                     KHIKALDTGPTLVKGLEQPVHVFQLQSRVSQQSEQVQRVEDVPFIARHNELHVLEMAL
                     NEASQHHGRCVAVCAEPGVGKSRLVNQFSQSIDREYFQLIETACDSHNRNTAYLPFTN
                     ILRYWLSVQETDKQLDIAQKLRDRVARMG"
BASE COUNT         1307 a         2020 c         1619 g         1107 t
ORIGIN      
        1 ctgaatgctt ccacagtttg acaagtatag aagtaataac cggcgctggc acccgctttt
       61 gcccgtccgg cccggcgtcg cctccaggct gcaccctccg ctataatgcg cagcccgctt
      121 ccaccgagac gcccaagacc catgctcccc gacagttacc agaaccgctt cagtggcatc
      181 gaccgcctgt atggcaaggg cgccctggac atcctgcgcc agcgtcacgt ggcagtggtc
      241 ggcatcggcg gcgtcggctc ctgggcggca gaggccctgg cccgctccgg cgtgggcgaa
      301 atcaccctgt tcgacctgga cgatatctgc gtctccaaca ccaaccgcca gattcatgcc
      361 accaccggca ccttcggcca gatgaaagtg gatgtgatgg ccgcgcgact gaaggccatc
      421 aaccccgacc tgatcgtgca tgccgaggcg gatttcgtca ctgccaccaa cgtggcggaa
      481 aaactcacga aacgcttcca cttcgtgctc gacgccaccg acaaggtcaa gcacaagact
      541 gccatgatcg tgcactgcaa gcgcaacaag attcccattt acgtggtggg cggcgccggc
      601 ggccagaccg atcccacccg catccagtat gccgacatga cccgcgccca ccaggacccc
      661 ctgctggccc tggtgcgcaa gaaactgcgg cagaatcacg gctacagcac caacctcaag
      721 cgccgcttca gcgtggaatg cgtgttttcc gacgagcagc tgatgttccc ccagccggac
      781 ggcagcgtct gcgccacccg cccggagctg gatgaggact ccggcagcat ccggctggac
      841 tgcgccggcg gcttcggcgc ctccacctgc gtcacggcca cctttggctt tgtcgccgtt
      901 tcccgtatca tccggaaact gctggacaaa gcatggaagg ccccctcccc ttcatccccc
      961 gaccgggacg aaacgtgacc gggttcaact gccggcagca caggatataa ctacaatcag
     1021 gactgacagt ttttagcggt attctcatga cttcattatt tgtgcgcggc tccttactga
     1081 cgctcgtcgt tggctctctg gcttcccccg tcgctttcgc tgcgacagac ctccacaaat
     1141 tcgccatgat cgacggcatc agccgtgaac tggtactgca ggaaaaccgg gagctgatgc
     1201 aatccctgcc ggtggaaaac ggcaccgcgg ttcactacac cggccagttg gtggactttc
     1261 ccgacagctg ggcgcggctt tcccgcattg acgggcaatg gcaaggggtc gtggtgaaca
     1321 gcggggagat cttcctggtc gaacagctgg ctgactcacc gcaggaagca acgtcccgca
     1381 gcgccgagtt cgccgtcagc gctgtaaaaa gttttgagga actgggcgac tgcgccgttg
     1441 cccacccacc agccggcggc agttccgcac cccaggcacg ggcgctgacg tcgcaggcgc
     1501 tggtaggtca ggccgtcagc gtgaactttc cggcctactg cagcgcaacc gtcaacggca
     1561 cctgtctggt ggcggaactc acggtggtgt tcgacgaaaa attcaagcag gcattcccga
     1621 gcaattacac cagccaggcc gcatccttgc tgaataccct cgacggcttc taccggcagg
     1681 atttcaagat cgtattccgt cacctgcggc tggatttcga caacggcagc acctttacca
     1741 ccagtcgcga tccctacgac ctgctcgccg acattggcga aaaacgcttc aacaacagca
     1801 tcagcagctt tgaccccaac aaacgctcat tgctgcatat ggtcaccgga cgggatttcc
     1861 gcttcgaaga caacggcgac attaacgacg acgtggtagg actggcctac agcccggact
     1921 atgacatcag caattatccc aactcgtttt acccggtact gtgcgacagc gatggcagcg
     1981 ccgtgggcac atcccagctg ctgtacagtg gcaacacacc cagcgctacc cgcaccgcac
     2041 tggtggttgc ccacgagatc ggccacaact taggtgccga acacgatggc actaccgaca
     2101 gcgtgaccgc ctccagctgc accgatgcca ccaaaatcat gaacccctac gtggtgtcca
     2161 atcccagcgg cttttccacc tgttcacaga gcaccatcgc caccaacatc agtcgcctgc
     2221 ctgccgtgga agcctgcttc gactttcccg tggacgccag cctggccgcg gtgtccggca
     2281 acctgaccca gaccacaccg gaaaccgcct tcattcagca gtacactgcg gggttcagca
     2341 ccgccaacgg ggttaacggt accgtcaccg tcagcggcac cctcaacggt ggcgacgcca
     2401 ctttcagcac cgtcaccctg gccggcaacg gctgcaccct cagcaacggc aaccagacct
     2461 acacctgcac caccggcagt ctggccagcc cgtccgcgct cgcggtgagc ctgaacgcca
     2521 attcgaccca cgtatccatc gcccacagca ttacgccatc cagcgccaac catctgtatg
     2581 acgtggacgc agccgacaac aacctcagtg acaccattct ggtcagggac gctgacctgc
     2641 cgcccaacag cctcaccgca acattgtcgg gcaacgacgt gcagctggcc tggcaggacc
     2701 acgccaccaa cgaacagggc tttgtggtgg aacgcaaaaa aggcgacggt gaatggaccg
     2761 taatggccgc cgcactggcc accaacagcg tcagctacac cgatgcagac gtcgcctcac
     2821 aaagcagtta cacctaccgc gtcagcgccc tgttcgatgg cgaacgctcg gccccctcca
     2881 atgaggtcag cattgacgtg agcattgccc cttctggcac catgcgcatc ggcggctccg
     2941 gcaaaggggg cggcggcagc ctgtcccccc tgctgctggc attcatggct ggaatggcga
     3001 gcctgttggc atttttgcag cgcaaatatt gatcagtcct gcacggcgac agcagggtga
     3061 ctacactttc cggtagttac cctggagctc gcccatgccc cacagccaag acgccaccct
     3121 gcggcgcctg atcgacatta cccagtccct ttcatcggaa caggataaat ggcgcctgct
     3181 ggaacgcatt ctggcggaaa gcatggccat cacccgggcc gacggtggca ccctgtacct
     3241 gatgcagaac gacgccgaag gccagtggct ggaatacggc attctgcaca accgctccct
     3301 gggtctggtc cagatcaacc gcagcggcag cgactcggcg ttgatgccca ttccggtgta
     3361 cgacctgcac accggcagcg ccaatcacca gaacatcgcc acccactgcg cactgacccg
     3421 ccgcaccatc aatatcagtg acgcctatga agccagccag ttcgatatca gtggcatccg
     3481 cgccttcgac gaactgttcg actaccgcac ccgctcgatg ctgacgctgc ccctgcagaa
     3541 tcatgccggc caggtgattg gcgtcatgca actggtcaac gccaccgcac cggacaccac
     3601 cggcgtggtg cccttcaccg atcccgccct gagcatcgtg caggcactgg catccctcgc
     3661 gtccatcatc ctggaaaaca gcctgatgct ggaattccag cgcgacctgc tgattcgcct
     3721 gtccagcgca ggaaacacga gcgaactgtt cgaacgcatt ctcgatgaag ccctcgccat
     3781 cacccgcgca gaaggcggct cgctatacct ggtaagggag ggcaacgcac cgcgactcga
     3841 atttgtgctg ctcaagaatc aggcattaaa actcaatctg ggaggacgcc atgggcagcc
     3901 ggtggacatg gcccccctgc tgctggaaaa gaacggcgag gccaacctgg gcaacatcgc
     3961 cacctacacc gccctgagcc gggaaaccgt caatatccag aatgcctatc acgacatccg
     4021 cttcgatttc tccggcatga aggcattcga taccgaccac cactaccgtt cccgctcttt
     4081 cctgtcagta ccactaatca atcatgccag cagcgttctc ggcgtactgc aactggtaaa
     4141 cgcccgtgac cccgacggcc agatcatggc ctttgacgaa cgccaggagc ccatcgtgca
     4201 ggcgctggcg tcctatgccg ccatcgcact cgaaaaccag cagctgcggg aggatcatgc
     4261 acgcctgctg gccgaggcca accaccatga ccaccgccaa aagcccgact gacttgtccc
     4321 ggatacaaag tcaatccacc cgttctggca atttttcaca ttcctgttgg cactaagtgg
     4381 gtaaccaagc cccggctaag ccactaccat tgaactcagg tggttgggga tctctgcgag
     4441 cactttggtt cctgactgtc tgccctggga tgcaagttcc aggtgcatca gtactatgcg
     4501 agcaccggga tctgttgggc aaggattgcc cagccatgca gatcccaagc gcgggcaagg
     4561 gttgcgagcc cctttcccgc gctttctatg tatgacggac agtgactgtt ttacggcact
     4621 gaatgtattc actcgacgct gatcaccgga tttcgacagc atcgcgtccc aagcccggaa
     4681 tagactcatg ttggcaagca agatgaatat cgacctcgca gtgcacgatg cgcagcagtg
     4741 gacagaatac gaaggcgtct ccaaagtgat ggtcgcggcc aacaggcgct ccatcctggt
     4801 cgttacgtcc tgcaatccag cggcaatcac cgccccgatt ccttcctttt tccacggatt
     4861 ccaggttcaa tttcaccagt cgacacaagc tccgcaaaca ttggaatcct gatcttcccg
     4921 ccgttatact ctcgatacgg atggacacaa gctggacagt gtcctttctg tcaggcgtca
     4981 gcctccgggc caacccggaa accctcttcg agagcagttc atgtattgcc aggcatgtgg
     5041 ggccgagaat cccaatttcc acgcattttg ctataaatgc ggaagcgcac ttaaggagtc
     5101 caaggatcag ccggagcatt tccggcagga accccgtgtg ccccatgccc gcgacctgga
     5161 gccggagctg aagaaagtct cggtcctgtt cgtcgacatc accaattccg ttcacctgat
     5221 ccacaagctg cggcctgaag acagcaccga atacctggaa cccaccctca acgccctcaa
     5281 acagagcgcg catcgctatg gcggcatgat caaccgggtg gacggcgatg gcatgatggt
     5341 gatcttcggc gcccccattg cctatgaaga ccacgcggtg cgggcctgct atgccgcgct
     5401 ggacatgctg aaggccgtga aatccgaatt cgatgccacc catattggcg tgcgggtcgg
     5461 catccattcc ggcgaagtgc tgatgaagcc cttctacaac gatttttcgg tggactacga
     5521 agcgctgggg cccacggtct acctggccca ccgcatggaa gtgctggcgt cccccaatac
     5581 tgcgatcatt tcccgggaaa cctatcgcct ggcccagaag cacatcaagg cgctggatac
     5641 cggccctacg ctggtgaaag ggctggaaca accggtgcac gtgttccagc tgcagagccg
     5701 cgtctcgcag cagagtgagc aggtgcagcg ggttgaggat gtgcctttca ttgcgcgcca
     5761 caacgaactg cacgtgctgg aaatggcgct caatgaagcc agccagcatc atggccgctg
     5821 cgtggcggtc tgtgccgaac caggcgtggg caaatcacga ctggtaaacc agttttccca
     5881 gagtatcgac cgcgaatact tccagctgat cgaaaccgcc tgcgattccc acaatcgcaa
     5941 caccgcctac ctgccattta ccaacatcct gcgctattgg ctcagcgtgc aggagaccga
     6001 caagcagctg gacattgcgc aaaagctgcg ggaccgggtg gcacgcatgg gtg
//
LOCUS       DASUEG010000739         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_558939, whole genome shotgun sequence.
ACCESSION   DASUEG010000739 DASUEG010000000
VERSION     DASUEG010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558939"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFX11_13970"
     CDS             complement(<1..214)
                     /locus_tag="VFX11_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4939779.1"
                     /translation="MKEVSRLPGCLVLAAGSHRRFGPSKLLQPLADGKALIQHTLDAL
                     KASGLPVALVHRPDDAALLDQIDAATI"
     gene            complement(211..1179)
                     /locus_tag="VFX11_13975"
     CDS             complement(211..1179)
                     /locus_tag="VFX11_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX4939780.1"
                     /translation="MQSSNEQVIRKLHDWLQADRFCWLISIAATWGSSPRPAGSLLVW
                     CAEEGALGSLSGGCVEDELLEKLRQDQFPRNRIHRETYGISAEEASRMRLPCGGSLSL
                     ILEPLSPSDANRQHAQQMVAMLERRQGFVRTTGTGAAVTSACCSTLHPATPQPVQLDD
                     LAFHNYLGPRYRLLLVGANQVAEFLADFARTLDFEILVCDPRPGAFDHWPHPFTTNHT
                     GMPDDVVRGFAHDPLSAIVTLSHDPRVDDMALMEALTCDAFYIGAMGSMRTTEKRLQR
                     LQQLDLTPAQLARLHAPVGLAIGSKTPAEIALSIAADLVRHVRRTP"
     gene            complement(1214..>2303)
                     /locus_tag="VFX11_13980"
     CDS             complement(1214..>2303)
                     /locus_tag="VFX11_13980"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4939781.1"
                     /translation="VSGLRRSQIKVHTTLIGGGFGRRLNVDYAVEAVHIAKASGLPVK
                     LVFSREDDIRHDFYRPAAHTELAAGLDTRGRITGWSHKNVCPAILTWSFQELAKAVLP
                     AWLPDGMVSGTAGLGPMLYGSMLADPPSTEGAMEYSYDVENVEVSHVQSDPGLRTGFW
                     RSVGHSINAFMIESFMDELAQKAGQDPVQFRLAHLSHNPRMAGVLKLVAEKGNWGKPS
                     VAGAKQGIAVHPSFGSYVAQLVEASVSGNQIKLHRVVVAADCGRIINPDIVVAQMEGG
                     VIFGLTAALKGEISLNKGVVEQSGFNDYQVLRMNEAPKVEVHLVNSNEEPGGVGEPGV
                     PPAAPALANALFAATGQRLRDLPLRLNS"
BASE COUNT          369 a          712 c          792 g          430 t
ORIGIN      
        1 ggatggtggc ggcgtcgatc tggtccagca gggcggcatc gtcggggcgg tggaccaggg
       61 cgacgggcag accgctggcc ttcagcgcat ccagcgtgtg ctggatcaga gccttgccgt
      121 ctgccagtgg ctgcagcagt ttggacgggc cgaagcggcg atgggagccg gctgccagca
      181 ccaggcagcc gggcaagcgc gagacttcct tcatggtgtc cggcggacgt gccgcaccag
      241 atccgccgcg atgctgagag caatttccgc cggcgtcttg ctgccgatgg ccagacccac
      301 cggtgcatgc aggcgggcaa gctgggcggg ggtgaggtcc agttgctgca gccgctgcag
      361 gcgtttttcc gtggtgcgca tcgagcccat ggcgccgatg tagaacgcat cgcaggtgag
      421 ggcttccatc agtgccatgt cgtcgacgcg gggatcgtgg gacagggtga cgatggcgct
      481 gaggggatca tgggcgaacc cccgcaccac gtcgtcgggc atgccggtgt ggttggtggt
      541 gaaggggtgc ggccagtggt cgaaggcacc ggggcggggg tcgcacacca ggatttcgaa
      601 atccagcgtg cgggcgaagt ccgccaggaa ttccgccacc tgattggcgc ccaccagcag
      661 caggcggtag cgggggccca ggtagttgtg gaaggcaaga tcatccagtt gcacgggttg
      721 cggtgtggca gggtgaagtg tgctgcaaca agcgctggtg actgcggcgc ccgtgccggt
      781 ggtgcgcacg aagccctgcc gccgttccag catcgccacc atctgctgcg catgctggcg
      841 gttggcatcg gatggtgaca gtggttccag aatcaggctg agggaaccac cgcagggcag
      901 gcgcatgcgg ctggcttcct cggcggaaat gccgtaggtc tcgcggtgga tgcggttacg
      961 gggaaactga tcctgccgca gtttctccag cagttcgtct tccacacagc cgccggacag
     1021 ggagcccagc gcgccttctt cggcgcacca gaccagcagc gatcccgccg gccggggcga
     1081 actgccccag gtggcggcga tgctgatcag ccagcagaac cggtcggcct gcagccagtc
     1141 gtgcagtttg cggatcacct gttcgttgct gctctgcatg gggctccttc aggttcaagc
     1201 tacgttaact ggtctaactg ttcagccgca gcggcagatc ccgcaggcgc tgcccggtgg
     1261 cggcaaacag tgcgttagcc agcgccggtg ctgccggagg tacgcccggc tcacccacgc
     1321 cgcccggttc ttcattgctg ttgaccagat gcacttcgac cttcggtgcc tcattcatgc
     1381 gcagcacctg gtaatcgttg aatccgctct gctccaccac gcccttgttc aggctgattt
     1441 cccctttcag cgcggcggtc aggccgaaga tcacgccgcc ttccatctgg gcgaccacga
     1501 tatccggatt gatgatgcgg ccgcagtccg cagccaccac tacccggtgc agtttgatct
     1561 ggttgccgct gacactggct tccaccagct gtgccacgta ggaaccgaac gacgggtgca
     1621 cggcgatacc ctgtttcgcc cctgccaccg acggcttgcc ccagttgcct ttttccgcca
     1681 ccagtttcag cacaccggcc atgcgcggat tgtggctcag gtgagccagg cggaattgca
     1741 ccggatcctg gccggccttt tgggccagtt cgtccatgaa gctttcgatc atgaaggcat
     1801 tgatggaatg gcccaccgag cgccagaagc cggtgcgcag gccgggatcg ctctgcacgt
     1861 ggctgacttc cacgttctcg acgtcgtagc tgtattccat ggcgccttcg gtactgggcg
     1921 ggtctgccag catcgaaccg tacagcatcg ggcccaggcc ggcggtgccg ctgaccatgc
     1981 cgtccggcag ccaggcgggc agtacggcct tggccagttc ctggaaggac caggtgagga
     2041 tcgccgggca gacgtttttg tgggaccagc cggtgatgcg gcccctggtg tccaggccgg
     2101 cggccagttc ggtatgtgcg gcggggcggt agaaatcgtg gcggatgtcg tcctcgcggc
     2161 tgaacaccag tttcaccggc aggccgctgg cttttgcgat gtgaacggct tccacggcgt
     2221 agtccacgtt gagacgccgg ccgaaaccac cgccgatcag ggtggtgtgt accttgatct
     2281 ggctgcggcg caggccactg act
//
LOCUS       DASUEG010000740         4478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1865218, whole genome shotgun sequence.
ACCESSION   DASUEG010000740 DASUEG010000000
VERSION     DASUEG010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865218"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFX11_13985"
     CDS             <1..813
                     /locus_tag="VFX11_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4939782.1"
                     /translation="SAEALDNRQAQRDQAASRLKALQAALNLAQLNLDFTRVEAPISG
                     RVSRALVTEGNYVSAGQTSLTSLVFTDRVYAYFDADEQTFLKFRQHHAEGAQVPVFMQ
                     LANESEFPHRGQIDFLDNRLNPQTGTIRARAVFDNREGRFTPGLFARLKLAVSGRYEA
                     VLINDRAVGTDLSNQFVLVVNGANEVSYRAVTLGSRLQGLRVVKDGLQDGEHIVVNGL
                     QRVRPGSQVQPQAVPMASADALARLAREHNTLLPAEPALSLRESLPASIPRG"
     gene            837..4019
                     /locus_tag="VFX11_13990"
     CDS             837..4019
                     /locus_tag="VFX11_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4939783.1"
                     /translation="MNLSRYFIQRPIFAAVLSMLILIAGGLSLFQLPITEYPEVVPPT
                     VVVSASYPGANPKVIAETVASPLEQAITGVEDMLYMSSQSTADGRLSLTVTFALGTNL
                     DDAQVQVQNRVSRALPRLPEDVQRIGVTTEKSSPDLTMVVHLYSPDERYDNLYLSNYG
                     ILNVKDELTRIDGVGDVQVFGAGEFSLRVWLDPEQVASLGLTTADVVAAIREQNRQAA
                     AGAIGAPPAASASDYQLLINVKGRLESEEEFGNIVVRMGPNGEITHLRDIARIELGSN
                     SYALRSLLDNQPAVALPIFQRPGSNAIEISDNVRATMARLKANFPEGIDYSIVYDPTV
                     FVRDSIKAVVQTLLEAILLVVIVVVLFLQTWRASIIPLVAVPVSLIGTFAVMHLAGFS
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNIELGKSPLEATQQAMKEVTGPIIATSLV
                     LSAVFIPIAFISGLTGQFYKQFALTIAISTLISTFNSLTLSPALAAVLLKPHGATPDR
                     PTRVINFLFGWLFRPFNRAFEKLGNGYGVTVKKLIRGSAIVVAVYGGLMLLTQQQFAH
                     TPTGFVPGQDKQYLVSFAQLPDAATLDRTEAVIRDMSAIVLKHPGVESAVAFPGLSIN
                     GFTNAPNAGIVFVTLKPFAQRKDPGLSAGAIAGELNQQFGAIQEAFVAIFPPPPVMGL
                     GTIGGFKLQIEDRGSFGYEELFNQTQAVLQKAWAAPELADMFSSFQINVPQLQVNVDR
                     EKAKSHGVAVNDIFDTLQVYLGSLYVNDFNRFGRTYQVNVQADQSFREDAEQISRLKV
                     RNQQGEMIPLGAFVDVKRSAGPDRVMHYNGYPTAEINGAPAPGYSSGQAEEAIAKILA
                     DTLPNGMTYEWTDLTYQQILAGNSAIYVFPLVVLLVFLVLAAQYESWSLPLAIILIVP
                     MTLLSAISGVILHGGDNNIFTQIGLIVLVGLAAKNAILIVEFAKDQEDAGLPLLDAVL
                     KASRLRLRPILMTSLAFIMGVVPLVLSSGAGAEMRQAMGTAVVWGMLGVTFFGLLLTP
                     VFYYLVRKAVAWQESRRRAAATAVVVPDHA"
     gene            4012..4158
                     /locus_tag="VFX11_13995"
     CDS             4012..4158
                     /locus_tag="VFX11_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939784.1"
                     /translation="MRNLLKQPAQYALEVWEGGAEEEPERRLATKLVLALLLLALAAL
                     TAGG"
     gene            complement(4171..>4478)
                     /locus_tag="VFX11_14000"
     CDS             complement(4171..>4478)
                     /locus_tag="VFX11_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012272987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4939785.1"
                     /translation="IEATPDTPDADPEMAAIEAGAQDLEPAENGATLFLTDPTDLDAV
                     SRALPGQGFTVISAQLGYRPKNPVKLDSDEARAEVEAFLEAIDADDDVQNVYVGLAD"
BASE COUNT          814 a         1405 c         1420 g          839 t
ORIGIN      
        1 tccgccgagg ccctggacaa ccggcaggcc cagcgcgacc aggccgcctc ccggctgaaa
       61 gcgctgcagg ccgcactgaa cctggcccag ctgaatctgg atttcacccg ggtggaggcg
      121 cccatctccg gccgggtatc ccgggcactg gtgacggaag gcaactatgt cagcgccggc
      181 cagaccagcc tcaccagcct ggtgttcacc gacagggtct atgcgtactt tgatgcggat
      241 gagcagacct tcctcaaatt ccgccagcac cacgcggaag gcgcccaggt gccggtgttc
      301 atgcaactgg ccaacgaaag cgaattcccc caccggggcc agatcgactt cctggacaac
      361 cggctgaatc cgcagaccgg caccattcgc gcccgggcgg tgttcgacaa tcgcgagggc
      421 cgcttcaccc ccggcctgtt cgcccgcctg aaactggcgg tgagtggccg ctatgaagcc
      481 gtgctgatca acgaccgcgc cgtgggcacc gacctgagca accagttcgt actggtggtg
      541 aatggtgcca acgaggtgag ctatcgggcg gtaaccctgg gttcgcgcct gcagggcctg
      601 cgggtagtga aggatggcct gcaggatggt gaacacatcg tggtgaacgg cctgcaacgg
      661 gtgcgccccg gcagccaggt gcaaccacag gcggtgccca tggcctccgc cgacgccctg
      721 gcgcggctgg cccgggaaca caacacgctg ctgccggcag aaccggccct cagcctccgc
      781 gaatccctgc ccgccagcat tccgcgcggt tgaacgcctg accaggagaa tccaccatga
      841 atctgtcccg ttatttcatc cagcgcccga tcttcgccgc cgtgctgtca atgctgatcc
      901 tgatcgccgg cggcctgtcc ctgttccagc tgcccatcac cgagtatccg gaagtggtgc
      961 cgcccaccgt cgtggtgtct gccagctacc ccggcgccaa ccccaaggtg atcgcggaaa
     1021 ccgtggcctc gccgctggaa caggcgatca ccggcgtgga ggacatgctg tacatgtcgt
     1081 cccagtccac cgccgatggc cgcctgtccc tcaccgtcac cttcgccctg ggcaccaatc
     1141 tggacgacgc ccaggtgcag gtgcagaacc gggtgagccg tgccctgccc cgcctgccgg
     1201 aggatgtgca gcgcattggc gtgaccacgg aaaaatcctc gccggacctg accatggtgg
     1261 tgcacctgta ctcgccggat gagcgttacg acaatttgta cctgtccaat tacggcattc
     1321 tcaacgtgaa ggatgaactg acgcggatcg acggcgtggg cgatgtgcag gtgttcggtg
     1381 ccggcgaatt ttcgctgcgg gtgtggctgg acccggaaca ggtagcgtca ctgggcctca
     1441 ccaccgccga tgtggtggct gcaatccgcg agcagaaccg tcaggcggcc gccggtgcca
     1501 tcggagcacc gccggccgcc agcgccagcg actaccagct gctgatcaac gtgaagggcc
     1561 ggctggaatc ggaagaggaa ttcggcaata tcgtggtgcg gatgggcccg aacggcgaga
     1621 ttacccacct gcgcgacatc gcccgcatcg agctgggttc caattcctac gcgctgcgtt
     1681 cgctgctgga caaccagccg gcggtggcgc tgccaatctt ccagcgcccc ggctccaatg
     1741 ccatcgagat ttccgacaac gtgcgcgcga ccatggcccg cctgaaagcg aattttccgg
     1801 aaggcatcga ctacagcatc gtctacgacc ccaccgtgtt cgtgcgcgat tccatcaagg
     1861 cggtggtgca gacattgctg gaagccatcc tgctcgtagt gatcgtggtg gtgctgttcc
     1921 tgcagacctg gcgcgcgtcg atcattccgc tggtggcggt gccagtatca ctgatcggta
     1981 ctttcgcggt gatgcacctg gcggggtttt cgctcaacgc gctgtcgctg ttcgggctgg
     2041 tgctggccat cggcatcgtg gtggacgacg ccatcgtggt ggtggaaaac gtggagcgca
     2101 atatcgagct gggcaagtcg ccgctggaag ccacgcagca ggccatgaag gaagtgaccg
     2161 gacccatcat cgccacctcg ctggtgctga gcgcggtatt tattcccatc gcattcattt
     2221 ccgggctgac aggccagttc tacaaacagt ttgcgctgac catcgcgatc tccacgctga
     2281 tttccacgtt caactcgctg accctgagcc cggcgctggc ggcagtgctg ctgaaacccc
     2341 acggcgccac gccggaccgc cccacccgcg tcatcaactt tttgttcggc tggctgtttc
     2401 gcccgttcaa ccgcgcattt gaaaaactcg gcaacggtta cggcgtcacg gtgaaaaaac
     2461 tgatccgcgg cagcgccatt gtggtggcgg tgtacggcgg gctgatgctg ctgacccaac
     2521 aacagtttgc ccatacgccc accggtttcg tgccggggca ggacaagcag tacctggtgt
     2581 cattcgcgca gttgccggat gcggccacgc tggaccggac cgaagcggtg atccgcgaca
     2641 tgtcggccat cgtgctgaag catcccggcg tggaatcggc ggtggcgttt ccgggcctgt
     2701 ccatcaacgg cttcaccaat gcgcccaatg cggggatcgt gttcgtgaca ctgaaaccct
     2761 ttgcacagcg caaagatccg ggcctgtccg ccggcgccat tgcgggtgaa ctgaaccagc
     2821 agttcggtgc aatccaggaa gccttcgtcg ccatcttccc gccccctccg gtgatgggac
     2881 tgggcaccat cggcggcttc aaactgcaga tcgaggaccg cggcagtttc ggctacgagg
     2941 agctgttcaa ccagacccag gccgtgctgc aaaaagcctg ggccgcaccg gaactggcgg
     3001 atatgttctc cagctttcag atcaatgtgc cgcaactgca ggtgaatgtg gaccgggaaa
     3061 aagccaagtc ccacggcgtg gcggtgaacg atatcttcga cacactgcag gtgtacctgg
     3121 gctcgctgta cgtgaatgac ttcaaccgtt tcggccgcac ctaccaggtg aatgtgcagg
     3181 cggaccagtc atttcgtgaa gacgccgagc agatcagccg tctgaaagtg cgcaaccagc
     3241 agggcgagat gattccgctg ggcgcttttg ttgacgtgaa gcgcagcgcc gggccggacc
     3301 gggtgatgca ttacaacggc tatcccactg ctgaaatcaa cggcgcaccg gcgccgggtt
     3361 acagttcggg tcaggcggag gaggcgatcg cgaaaatcct ggcggacacc cttcccaacg
     3421 gcatgacgta cgagtggacc gatctcacct accagcagat tctggcgggc aattcggcaa
     3481 tctatgtgtt cccgctggtg gtgctgctgg tgttcctggt gctggcggcg caatacgaaa
     3541 gctggtcact gcccctggcg atcatcctga tcgtgccgat gaccttgctg tccgccattt
     3601 ccggcgtgat cctgcacggt ggcgacaaca acatcttcac ccagatcggc ctgatcgtgc
     3661 tggtggggct ggcggcgaaa aacgcgatcc tgatcgtgga attcgccaag gatcaggaag
     3721 atgccgggct gccattactg gacgccgtgc tgaaagccag ccgcctgcgg ttgcggccga
     3781 tcctgatgac gtcgctggcc ttcatcatgg gcgtggtacc gctggtattg tcatccggcg
     3841 ccggcgcgga aatgcgccag gccatgggca cggcggtggt ctggggcatg ttgggcgtga
     3901 cgttcttcgg tctgctgctg acgccggtgt tttattacct ggtgcggaaa gcggtggcct
     3961 ggcaggaatc gcgccggcgc gctgcggcaa cagcggtggt ggtgccggat catgcgtaat
     4021 ctgctgaaac agccggcaca gtatgcactg gaagtatggg aaggcggagc ggaggaagaa
     4081 ccggaacggc gactggcgac aaagctggta ctggcgcttc tgctgctggc cctggccgcc
     4141 ctcacggcgg gcggctaggg ccggagggtt tcagtcggca aggcctacat agacattctg
     4201 cacatcgtcg tcggcgtcga tggcttccag aaaggcttcc acttcggcgc gggcttcgtc
     4261 actgtccagt ttcaccggat ttttcggccg gtaacccagc tgggcggata tcacggtgaa
     4321 accctgaccc ggcaatgccc ggctcaccgc atccagatcg gtgggatcgg tgaggaacag
     4381 ggtggcgccg ttttccgccg gttccagatc ctgggcaccg gcttcaatgg ccgccatttc
     4441 cggatcggca tccggggtgt ccggggttgc ttcgatca
//
LOCUS       DASUEG010000741         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1294296, whole genome shotgun sequence.
ACCESSION   DASUEG010000741 DASUEG010000000
VERSION     DASUEG010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294296"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFX11_14005"
     CDS             complement(<1..601)
                     /locus_tag="VFX11_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4939786.1"
                     /translation="MMKSFLRLVGGCLVGVALQATAAELKPGHPDVYPVKKGDTLWDI
                     SSAFLNDPWLWPELWQVNPQIENPHLIYPGDLIKLVYVDGKPVLTLERGQRTVTYKKG
                     DTIKLSPQVRTEQLDTVIPAIPLEYIQGFLVDNRVLSEEEINLSPYLVAGDEKHIVMG
                     VGDYVYGRGDWSQPHQNYGVFRKGPVYMDPDTKEVLGIAA"
     gene            883..1401
                     /gene="def"
                     /locus_tag="VFX11_14010"
     CDS             883..1401
                     /gene="def"
                     /locus_tag="VFX11_14010"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4939787.1"
                     /translation="MALLDILEFPDPRLRTKAKPVTVVDDKIRKLCDDMLETMYAAPG
                     IGLAATQVNVHERIVVIDTSEEKNQPLVLINPTFEVIEGEQQYDEGCLSVPGFYETVT
                     RAEKIRLKALGRDGQPYELVADGLLSVCIQHELDHLDGKLFVDHISKLKRDRIRKKLE
                     KEHKEQEKVRAR"
     gene            1450..2385
                     /gene="fmt"
                     /locus_tag="VFX11_14015"
     CDS             1450..2385
                     /gene="fmt"
                     /locus_tag="VFX11_14015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX4939788.1"
                     /translation="MNLIFAGTPEFAAIQLQALLGSRHRVIACYTQPDRPAGRGRKLT
                     PSPVKALAQEHGIPVYQPVNFKNADDVAQLQALNADLMVVVAYGLILPKAVLDAPRLG
                     CINVHASILPRWRGAAPIQRAIAAGDRETGVTLMQMDVGLDTGAMLLKVTTPISADDT
                     GGSLHDRLAQIGAPALLETLEQLAAGAAKPQAQDNSLATYAHKLTKEEAKLDWTRSAA
                     DLHNLIRAFNPWPVTHTELDGDTIRVWKAERQTGNHQAAPGTLLDVSRDGVTVATGDG
                     LLRLTELQLPGGKCLPVAALLNSRADWLQAGKRFL"
     gene            2422..3771
                     /gene="rsmB"
                     /locus_tag="VFX11_14020"
     CDS             2422..3771
                     /gene="rsmB"
                     /locus_tag="VFX11_14020"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX4939789.1"
                     /translation="MRLNAADSTIKNARALAAERLAQVLPLNRQNTQPKSLAELLPQL
                     TEQEKDQGLVQELCFGVCRWFTRLDKLAAPLMRQPFKDRDADLHALLLVGLYQMFYLR
                     IPDHAAIAETVEACRQLKKEWAVKVINGMLRSAQREKQNLEQQLPDSLAIQTSHPQWL
                     IDGLAAAWPDQLNAILRANNEPGPMCLRVNRLRTSREDYLRLLKTAGIAARAGELADS
                     AVYLEQAVGVQQLPHFNDGWVSVQDEAAQLSAFLLNPQPGERVLDACAAPGGKTGHLL
                     ELQPHLGELVALDADAKRLQRVEQNLQRLQLHASVVHSTLEEYATHHDGPRFDRILLD
                     APCSATGVIRRHPDIKWLRKRSDIGKLAATQLRLLEMAFGLLKPGGTLLYATCSVLPQ
                     ENMRVVHAFLKQQKNAVEELIVAPWGEAADCGRQLFPQIGRHDGFYYARVKRSAESA"
     gene            3768..>4751
                     /gene="trkA"
                     /locus_tag="VFX11_14025"
     CDS             3768..>4751
                     /gene="trkA"
                     /locus_tag="VFX11_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX4939790.1"
                     /translation="MKIIILGAGQVGGTLAANLASEHNDITVVDTNAERLHELQDRFD
                     IRTVQGMASYPSVLRQAGADDADMLIAVTNSDEINMVACQVAYSLHRTPTKIARVRSA
                     SYLNRNGLFGIDAFPIDVIISPEQVVTHYITRLIEHPGALQVLDFAEGKAQLVAVRAY
                     FNGPLVGRELRFLRQHMPSIDTRVAAIFRRDRPIIPEGTTVIEADDEVFFIAAKNHIR
                     AVMSELRGLEHNYKRILIAGGGNIGERLCDALENRYQVKIIERNPGRCAFLAEKLNKT
                     VVIRGNASDQDLLLEESIEETDIFLAVTNDDEANIMSSMLAKRLGARKVMCL"
BASE COUNT          974 a         1519 c         1426 g          832 t
ORIGIN      
        1 gtgcggcaat acccagcact tccttggtgt cgggatccat gtaaacaggg cccttgcgga
       61 acacaccgta gttctggtgg ggctgtgacc agtcaccgcg gccatacaca taatcgccca
      121 cacccatcac aatgtgtttc tcgtcgccgg cgaccagata gggcgacagg ttgatctctt
      181 cctcgctgag cacccggtta tccaccagga agccctggat atattccagc ggaatcgcgg
      241 ggatcacggt atccagctgc tctgtccgca cctgcgggct cagcttgatg gtgtcgccct
      301 tcttgtaggt aacggtgcgc tgaccccgtt ccagggtcag caccggcttg ccatcgacat
      361 agaccagctt gatcaggtcg cctggataaa tcaggtgggg attctcgatc tgggggttga
      421 cctgccacag ttccggccat aaccagggat cattcaggaa cgcgctggaa atgtcccaca
      481 gcgtatcgcc cttcttgacg gggtacacgt ccggatgccc aggcttcagc tctgccgccg
      541 tagcctgcag ggcgacacca accagacagc cgccgacgag cctgagaaat gatttcatca
      601 tggtgagaat ccctttatca ttatgaacgc ctggcgccag gcagctatat ttgcttgttt
      661 gtgcttgttc acctgcggaa aagcggcttg ttctacaggc attcccgcag aatcacccgc
      721 aatcgagcgc aagtttaact atagacttaa aaccttgttt tttaactata gcccgaactt
      781 tcgcacaggc aggcgttttt cccgccgtgc cgcgcaaatc ctgtacgatc ataatcttag
      841 caacagtttt catagttata ctagttaagc ggcgttctca agatggcatt gctggatatt
      901 ctcgaatttc ctgatcctcg tctgcgtaca aaggccaagc cggtcacagt tgttgacgac
      961 aaaatacgca aattatgtga cgacatgctg gaaaccatgt atgccgcgcc cgggatcggg
     1021 ctggctgcaa cccaggtcaa cgtgcacgaa cgcatcgtgg tgatcgacac cagcgaggag
     1081 aaaaaccagc ccctggtgct gatcaatccc accttcgagg tgattgaggg cgagcagcaa
     1141 tatgacgaag gctgcctgtc ggtgccgggg ttctatgaaa ccgtgacccg ggcagaaaag
     1201 atccgcctga aggccctggg ccgtgacggg cagccgtacg aactggtggc ggacggcctg
     1261 ctgtcggtgt gcatccagca cgaactggat cacctggacg gcaaactgtt cgtggaccat
     1321 atctccaagc tcaagcgcga ccgcatccgc aagaagctgg agaaggaaca caaggaacag
     1381 gaaaaagtgc gtgcccgctg aggccgcgat ccttcccgcc cggagacttt ccaccccgtg
     1441 acagctcccc tgaacctgat ttttgccggt acgccggagt tcgccgccat ccagctgcag
     1501 gccctgctcg gctcccgcca ccgggtgatc gcctgctaca cccagccgga ccgtccggcc
     1561 ggacgtggcc gcaagctgac ccccagcccg gtaaaggccc tggcccagga acacggcatt
     1621 ccggtgtacc agccagtgaa tttcaaaaac gccgacgatg tggcgcagct gcaggcactg
     1681 aacgccgacc tgatggtggt ggtggcctac ggcctgatcc tgcccaaggc ggtgctggat
     1741 gctccccggc tgggctgcat caatgtgcat gcgtcgatcc tgccgcgctg gcgcggcgcg
     1801 gcaccaatcc agcgggcgat tgcggcgggc gaccgtgaaa ccggcgtcac cctgatgcag
     1861 atggacgtgg gcctggacac cggtgccatg ctgctgaaag tcaccacacc catttctgcc
     1921 gacgacaccg gcggcagcct gcacgaccgg ctggcacaga tcggcgcgcc ggcgttgctg
     1981 gaaaccctgg agcagctggc ggccggcgcc gcgaaaccgc aagcacagga caacagcctg
     2041 gccacctacg cccacaagct caccaaggaa gaggcgaagc tggactggac ccggtccgcc
     2101 gccgacctgc acaacctgat ccgcgccttc aacccctggc ccgtgaccca cacggaactg
     2161 gacggcgaca ccatccgcgt ctggaaagcc gagcgccaga ccggcaatca tcaggccgct
     2221 cccggcaccc tgctggatgt atcccgtgac ggcgtcaccg tggcaacggg tgatggcctg
     2281 ctgcggctga ccgaactgca actgcccggc ggcaagtgcc tgccggtggc ggcccttctc
     2341 aattccaggg ccgactggct gcaagccggg aagcgcttcc tgtgagcagc actcttttcc
     2401 tgcaccagga ccttgcctga catgcgtctg aacgccgccg acagcaccat caaaaacgcc
     2461 cgcgccctgg ctgccgaacg actggcacag gtgctgcccc tgaaccgcca gaacacccag
     2521 cccaaatccc tggcggaact gctgccgcaa ctgaccgagc aggaaaaaga ccagggcctg
     2581 gtgcaggaac tctgcttcgg cgtgtgccgc tggttcaccc gcctggacaa attggccgcg
     2641 ccgctgatgc gccagccgtt caaggatcgc gacgccgacc tgcacgcact gctgctggtt
     2701 ggcctgtacc agatgttcta cctgcgcatt cccgatcacg ctgccattgc cgagacggtg
     2761 gaagcctgcc gccagctgaa aaaggaatgg gcggtgaaag tgatcaacgg catgctgcgc
     2821 agtgcccagc gggaaaagca gaatctggag caacagctgc cggattcgct ggcgatccag
     2881 acctcgcatc cgcaatggct gatcgacggc ctcgccgcgg cctggcccga ccagctgaac
     2941 gccattctgc gcgccaacaa tgaacccggc cccatgtgcc tgcgggtgaa ccggctgcgg
     3001 acctcccgcg aggactatct gcgtctgctg aaaacggcgg ggattgcggc gcgggcgggg
     3061 gaactggcgg acagcgcggt gtatcttgag caggccgtgg gcgtgcagca gctgccccat
     3121 ttcaatgacg gctgggtgtc ggtgcaggat gaagcggcgc aactgagtgc cttcctgctc
     3181 aatccccagc ctggcgagcg cgtgctggac gcctgcgccg cgcccggcgg caaaaccggt
     3241 catctgctgg agctgcagcc gcacctgggc gaactggtgg cgctggacgc cgacgccaag
     3301 cgcctgcagc gggtggaaca gaatctgcaa cgcctgcaat tgcacgccag cgtggtgcat
     3361 tccacactgg aagaatacgc gacccaccac gacgggccgc gcttcgaccg catcctgctg
     3421 gacgcgccct gttccgccac cggcgtgatc cgccgtcatc cggatatcaa gtggctgcgc
     3481 aaacgcagcg atatcggcaa gctggcggcc acgcagttgc ggctgctgga aatggcgttc
     3541 ggactgctga agcccggcgg caccctgctg tacgccacct gctcggtatt gccgcaggaa
     3601 aacatgcggg tggtgcacgc ttttctgaag cagcagaaaa atgcggtgga agagttgatc
     3661 gtggcaccct ggggcgaagc ggcggactgc ggccggcaac tgtttccaca gattggccgt
     3721 catgacggat tttattatgc gcgggtgaaa cggagcgcgg agtcagcatg aaaatcatca
     3781 tcctcggagc aggacaggtt ggcggcacgc tggcagcgaa cctcgccagc gaacacaacg
     3841 acatcacggt ggtggacacc aacgccgagc gcctgcatga actgcaggac cgcttcgaca
     3901 tccgcaccgt gcagggcatg gcgtcctacc ccagcgtgct gcgccaggcc ggtgccgatg
     3961 acgctgacat gctgatcgcc gtcaccaaca gcgacgagat caacatggtg gcctgccagg
     4021 tggcgtactc gctgcaccgc acacccacca agattgcccg cgtgcgttcc gccagctatc
     4081 tgaaccgcaa cggtctgttc ggcatcgacg cctttcccat cgacgtgatc atttcccccg
     4141 agcaggtggt gacccattac atcacccgcc tgatcgaaca tccgggcgca ctgcaggtgc
     4201 tggacttcgc ggagggcaag gcgcagctgg tggcggtgcg cgcgtacttc aacgggccgc
     4261 tggtgggccg cgaactgcgc ttcctgcgcc agcacatgcc cagcatcgac acccgcgtgg
     4321 ccgcgatttt ccgccgtgac cgccccatca ttcccgaagg caccacggtg atcgaggccg
     4381 atgacgaagt atttttcatc gccgcgaaaa atcatatccg cgcggtcatg agcgagctgc
     4441 gcgggctgga acacaactac aagcgcattc tcatcgccgg cggcggcaac atcggcgagc
     4501 gcctgtgcga tgcgctggaa aaccgctacc aggtgaaaat catcgagcgc aatccgggcc
     4561 gctgcgcgtt cctggcggaa aaactcaaca agacggtggt gatccgcggc aatgcgtcgg
     4621 accaggatct gctgctggaa gaaagcatcg aggaaacgga tatttttctc gccgtcacca
     4681 acgacgatga agccaacatc atgtcgtcga tgctggccaa gcgcctgggc gcgcgcaagg
     4741 tgatgtgcct g
//
LOCUS       DASUEG010000742         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_834311, whole genome shotgun sequence.
ACCESSION   DASUEG010000742 DASUEG010000000
VERSION     DASUEG010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834311"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFX11_14030"
     CDS             <1..379
                     /locus_tag="VFX11_14030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468139.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX4939791.1"
                     /translation="GEKAESKVDQSWYRETYIPVVQQRGAAIIKARGASSAASAANAA
                     LNHMRDWALGTPANDWVSMGVYSDGSYGIDKGLIYSFPCVCKNGDWEIVQGLDISEFS
                     REKMKVTEKELQEERDAVAHLLP"
     gene            complement(484..972)
                     /gene="rraA"
                     /locus_tag="VFX11_14035"
     CDS             complement(484..972)
                     /gene="rraA"
                     /locus_tag="VFX11_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX4939792.1"
                     /translation="MNIITCDLSDAYPDITRVVEPIFTNYGGRSAFGGEILTIKCHED
                     NSLVKEHVGNPGNGRVMVVDGGGSLRCALLGDMLAANAVKNGWAGIIIYGCIRDVDEI
                     RKMDLGVQALRTIPIKSNRQGRGDLNIPVTFGGVTFNPGEFVYADNNGIIVANQALKM
                     PG"
     gene            complement(1009..1686)
                     /locus_tag="VFX11_14040"
     CDS             complement(1009..1686)
                     /locus_tag="VFX11_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX4939793.1"
                     /translation="MKVLLLGATGLIGRHVLEGLLAAPQVSELIAPTRRTLSNTHPRL
                     RNVLIDFDRLDEYPELFDVTTIVCCLGTTMKQAGSKENFRKVDYQYCRDAAELGRAQR
                     ARAFLLVSAIGSSATSPVYYSRVKGELEQHLIELEYNHLSLYRPSMLLGERSEFRLGE
                     KLYSKLTPLIDPFMRGPLRHYHAIPARTVAQAMVNECLALGEVIPQGPKVNVYPYDKI
                     VKLAQSR"
     gene            complement(1794..>3448)
                     /locus_tag="VFX11_14045"
     CDS             complement(1794..>3448)
                     /locus_tag="VFX11_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4939794.1"
                     /translation="LAHAVNEANNASQIKTEFLASITHEIRTPMNNVLGMLALLDDTD
                     LSPQQQELQKVAVHSGEALLSLMDDILDFSRISSGQVQLEEVVFNLKRCIDQTLELMG
                     PRAHDKGLELSCVYDGNLPVRVKGDPERLAQLINNLVTNAIKYSAGTDVLLSVQMQRL
                     SETEGELKVTVTDNGKGIDPVLQDRLFDAFSKNSAQNNYAEAGTGLGLAISKGLAECM
                     QGEIGFSSAPEQGASFWFTARLRLSTQQAQKPTELRELMNSHILLVDIDGGLRAALMA
                     ELAGWQMDVRFASSQFNPVETLLAAQRAGMPYQLVFINLPIKQKTSLELCQEIRQHAE
                     LRNSHLIVLASLAQRADAARMKADIAQGVEWLSKPVTRDKLVRALTQSFNLQEAEGEG
                     LEKLDAALLSGSEGRRILLVEDNAVNQMVARGMLTRLGYLVTSVANGKEALGLLEDKT
                     FDLILMDCLMPVLDGYETTRIWREKEKEKGGHLPIVAMTASVVEGEQQRCLSTGMDDY
                     LSKPVNIDELSAKLRHWLGADKAAAPELEEGVTPRDPQRRTA"
BASE COUNT          679 a         1050 c          996 g          723 t
ORIGIN      
        1 gggcgaaaag gcagagtcca aagtggacca gagctggtac cgcgaaacct acatccctgt
       61 ggtgcagcag cgtggcgcgg ccatcatcaa ggcccgtggc gcttcctcgg ccgcttccgc
      121 tgccaacgcc gccctgaacc acatgcgtga ctgggccctg ggcactccgg ccaacgactg
      181 ggtcagcatg ggcgtgtaca gcgacggttc ctacggcatc gacaagggcc tgatctactc
      241 tttcccctgt gtgtgcaaga acggcgactg ggaaatcgta cagggtctgg acatcagcga
      301 attcagccgc gagaaaatga aagtgactga aaaggaactg caggaagagc gcgacgccgt
      361 tgcgcacctg ctgccgtaaa tcgttctgcc ttgtgcagat gacgctgaaa acaaaaaacc
      421 ccgcattgcg gggttttttt atggatgcct tttccgtgcc taatgcgcca acaccgacac
      481 gtttcagccc ggcatcttca gcgcctggtt cgccacaatg atgccattgt tgtcggcata
      541 gacgaattca cccggattga acgtgacgcc accgaacgtc accggaatat tcagatcgcc
      601 ccgaccctgg cgattgctct tgatcggaat ggtgcgcaat gcctgcacgc ccagatccat
      661 tttgcggatt tcgtccacgt cgcggatgca gccataaatg atgatcccgg cccagccgtt
      721 tttcaccgca ttcgccgcca gcatgtcgcc cagcagcgca caacgcagcg agccaccgcc
      781 gtccaccacc atcacgcggc cgttgcccgg gttgccgacg tgttccttca ccagtgaatt
      841 atcctcgtga cacttgatgg tgaggatctc gccgccgaat gcgcttctgc cgccgtagtt
      901 ggtgaagatc ggctccacca cacgggtaat atcggggtag gcgtcggaca ggtcgcaggt
      961 gatgatgttc aagtgaagct cctccggtag atacaacgac gggtgtcctc agcgggactg
     1021 ggccagttta acaattttgt cgtagggata aacattcacc ttggggccct gcggtatgac
     1081 ttcacccagt gccaggcact cattcaccat tgcctgcgcc acggtgcggg cgggaatggc
     1141 gtgatagtgg cgcaggggtc cgcgcatgaa gggatcaatc agcggcgtga gtttgctgta
     1201 gagtttttct cccagccgga actcgctccg ctcacccagc agcatggagg gccgatacag
     1261 cgaaaggtgg ttgtactcca gctcgatcag gtgctgttcc agctcgcctt tcacacggga
     1321 ataatagacc ggggacgtgg ccgacgatcc aatggccgat accagcagaa acgcccgcgc
     1381 ccgctgcgca cgccccagtt cggcggcatc gcgacagtac tggtaatcca ccttgcggaa
     1441 attttctttc gatccggcct gtttcatggt ggtacccagg cagcagacga tagtggtgac
     1501 gtcaaacagt tccggatatt cgtcaaggcg gtcgaaatcg atcagcacat tgcgcaatct
     1561 gggatgggta ttggacagcg tgcgacgagt gggcgcaatc agttcgctca cctggggcgc
     1621 agccagcaac ccttccagta cgtgacggcc aatcaggccg gtggcgccca gcaacagcac
     1681 tttcatgtgc gtcgttcccc gcgttggttt ggccggtatc cggcaagatt atcagaatgg
     1741 cggcggtatt ctagcgacaa tctgcgggaa agaaacgggg ttgcgaggaa tgttcaggcc
     1801 gtgcggcgct ggggatcgcg cggtgtaaca ccttcttcca gctcgggcgc agccgctttg
     1861 tccgcaccca gccagtgccg cagtttggcg ctgagttcgt caatattgac cggcttggac
     1921 agatagtcat ccatccccgt ggacaggcag cgctgctgct cgccttccac aacactggct
     1981 gtcatggcca cgatgggcag gtgtcccccc ttctctttct ccttttcccg ccagatccgc
     2041 gtggtttcgt aaccgtcgag aaccggcatc aggcagtcca tcaggatcag gtcgaaggtt
     2101 ttgtcctcca gcagacccaa cgcttccttg ccattggcca ccgacgtaac cagatacccc
     2161 agccgggtga gcatgcctcg cgccaccatc tggttgacgg cattgtcctc caccagcagg
     2221 atgcgacggc cttcgctgcc ggacagcagt gcagcatcga gcttttccag cccctcgcct
     2281 tcggcttcct gcagattgaa ggactgggtc agggcccgta ccagcttgtc gcgggtgacg
     2341 ggtttgctga gccattccac gccctgggca atatcggcct tcatgcgcgc cgcatcggcc
     2401 cgctgcgcca gcgatgccag cacgatcaga tgggaattgc gcagctccgc gtgctggcgg
     2461 atctcctgac agagctccag cgacgttttc tgcttgatcg gcagattgat aaacaccagc
     2521 tggtacggca tgccggcgcg ctgtgccgcc agcagggttt ccaccggatt gaactggctg
     2581 gaagcaaaac ggacgtccat ctgccagccc gccagttccg ccatcagtgc ggcccgcaag
     2641 ccaccgtcga tatccaccag aaggatgtgt gagttcatca gctccctgag ctcggtgggc
     2701 ttctgggcct gctgggtgga cagtctcaga cgcgccgtaa accagaacga ggcaccctgc
     2761 tccggcgcgc tggaaaagcc gatttctccc tgcatgcatt cggcaagccc cttgctgatg
     2821 gcaagcccca atccggtacc ggcttcggca taattgttct gggcggaatt cttcgaaaat
     2881 gcgtcgaaca gacggtcctg caggaccgga tcgatccctt tgccattgtc cgtcacggtg
     2941 actttcagtt cgccttcggt ttccgacagg cgctgcatct gcaccgagag cagcacatcg
     3001 gtaccggcgc tgtacttgat tgcattggtc acgaggttat tgatcaactg cgcaagacgc
     3061 tcagggtcgc ccttgacgcg caccggcaga ttgccatcgt aaacacagct caattccagg
     3121 cctttatcgt gggcccgtgg gcccatcagt tccagggtct ggtcgatgca gcgcttcaaa
     3181 ttgaacacca cttcctccag ctgcacctgc ccggaagaaa tgcgggagaa atcgaggatg
     3241 tcatccatca gactcagcag cgcctcgccg gagtgaaccg ccaccttctg cagttcctgt
     3301 tgctgggggc tgagatcggt gtcgtccagc aacgccagca tgcccagcac gttattcatc
     3361 ggcgtgcgaa tttcgtgggt gatgctggcc aggaattcgg ttttgatctg gctggcgttg
     3421 ttggcttcgt tcaccgcatg cgccagat
//
LOCUS       DASUEG010000743         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_713934, whole genome shotgun sequence.
ACCESSION   DASUEG010000743 DASUEG010000000
VERSION     DASUEG010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713934"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFX11_14050"
     CDS             <1..532
                     /locus_tag="VFX11_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4939795.1"
                     /translation="FASLMFCDSFHADVHAGNLLVLRDGRVGFIDFGIVGRIRPQTWE
                     SMSKFIWAIGQNNYPEMAEAMVGIGMTDQEVDVKRLTRDIENLYSSMDKMMPDMDIHN
                     PDFVSINEHEVNRVMMDLVGVGKRHGIHFPREFALLLKQLLYFDRYVRLLAIDANIYM
                     DDRLNITPEMGWTSIH"
     gene            543..728
                     /locus_tag="VFX11_14055"
     CDS             543..728
                     /locus_tag="VFX11_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939796.1"
                     /translation="MQPHNAKQEALEAIQRLPDNVDFEEIVYRFYVLNKIHQGMQDIE
                     AGRTISHEDLVREIERW"
     gene            768..1010
                     /locus_tag="VFX11_14060"
     CDS             768..1010
                     /locus_tag="VFX11_14060"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX4939797.1"
                     /translation="MIILPRRPLSTPGKVVSAFVDKAASLSDHPQPGKVVREVDDPNL
                     REVSVHSWRMVSHLRNQKIYVVTLIHKRRNVGAEDI"
     gene            complement(1097..2050)
                     /locus_tag="VFX11_14065"
     CDS             complement(1097..2050)
                     /locus_tag="VFX11_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939798.1"
                     /translation="MIHKQKPEILITGAGGALAQQVIQQLKRDYHVVLVDFRRRVPLE
                     EGMSSYRVEYNKRGFEDIFRKHKIEGVIHLGRMGLYESTHRNRYNHNVLGTQRLLDLS
                     HKYGVHKVVVLSTFFVYGASPYNPALLTEEAPLKASELTMDLVDSVELENLANIYLWK
                     YPELNITILRPCNIAGPGVLNSMSLLLSSKFAPVLMGFSPMMQFIHVDDMGHAVALAF
                     RKNKPGVYNVTTDDWISYQEALKLCGCNRVPIPSLPEWLPKAISKRADWKGWPPYLIN
                     YYKFPVIIDGSLFNKTFGFRPKKSLDDIFTYYREKKEMGIL"
     gene            complement(2047..2865)
                     /locus_tag="VFX11_14070"
     CDS             complement(2047..2865)
                     /locus_tag="VFX11_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4939799.1"
                     /translation="MSIRDFLKRHFVSAETEAIINRIPKPVGSFGYDPWGYNSDTTKI
                     ATAALKPFYEKYFRVEAFGLENVPTSGRLLVIANHSGQLPIDGTLVGYAMATNPHGPR
                     SLRVMIERWFPTMPFIGNIMNEMGGVVGDPENCEKMLRSEEAIIVFPEGVRGAGKTWD
                     KRYKLQRFGLGFMHLAITERTPILPVGVVGCEETMPTPFHLKTLGRLLGLPYLPVTTP
                     IPLPAKVRIYFGKPMLFEGPVTTEDDVAIKVEQVKDEINKLIARGLKEREGWFK"
     gene            complement(2862..>3118)
                     /locus_tag="VFX11_14075"
     CDS             complement(2862..>3118)
                     /locus_tag="VFX11_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939800.1"
                     /translation="SQVLYRPGWTKPAHNNKNLSPSFVIPVRQIMAMFHLGHCHCIRP
                     YVAVLFCLYEPDCGSGFVRPPCLREHDGRAFKGIYEVASA"
BASE COUNT          675 a          881 c          866 g          696 t
ORIGIN      
        1 gttcgccagc ctgatgttct gcgattcctt ccacgccgac gtgcacgccg gcaacctgct
       61 ggtcctgcgc gacggccgcg tcggcttcat cgacttcggc atcgtcggcc gcatccgccc
      121 gcagacctgg gaatccatgt cgaaattcat ctgggcgatt ggccagaaca actaccccga
      181 aatggcggaa gccatggtgg gcatcggcat gacggatcag gaagtggacg tgaaacgcct
      241 gacccgcgac attgaaaacc tgtactcgtc aatggacaag atgatgccgg acatggacat
      301 ccacaatccg gatttcgtgt cgatcaacga gcatgaggtg aaccgggtga tgatggatct
      361 ggtgggcgtg ggcaagcgac atgggattca ttttccgcgc gagtttgcgt tgttgctgaa
      421 gcagttgctg tactttgacc ggtatgtgcg gttgctggcg atcgatgcga atatttatat
      481 ggatgatagg ttgaatatta cgccggagat ggggtggact tcgatacatt gagtctgcca
      541 ccatgcaacc ccataacgca aaacaggaag cactggaagc tattcagcgt ttgccggata
      601 acgtggactt tgaagagatc gtgtatcgct tctacgtgct caacaagatt caccagggca
      661 tgcaggatat tgaagcaggc cgcaccatat cccatgaaga tctggtgcgc gagatcgagc
      721 gttggtgatt tggacacccc gcacccgttc cgatttgaaa gcgattcatg attatattgc
      781 ccaggaggcc cctatcaacg ccaggtaaag tggtaagcgc ttttgtcgac aaagccgctt
      841 ccctgagcga ccatccacag cccggaaagg ttgtgcgcga agtggatgat cccaatttac
      901 gtgaagtcag tgtccattcc tggcgcatgg tgtctcattt gcgcaaccag aaaatttatg
      961 ttgtcacgct gatccacaaa cggcgaaatg ttggtgctga agatatttaa gcccctcgaa
     1021 aaaatcccaa aacaaaaacg gcggccactt ttccagtgcc gccgttttca tttccgcctt
     1081 cagcgaattc aaagcctcac aaaatcccca tctccttctt ctcccgataa tacgtaaaaa
     1141 tatcatcgag cgattttttc ggcctgaatc caaacgtctt gttgaacagc gacccgtcga
     1201 tgatcaccgg aaacttgtaa tagttgatca gatacggcgg ccagcctttc cagtccgccc
     1261 gtttgctgat ggctttcggc agccactccg gcagcgacgg aatcggcacg cgattgcagc
     1321 cgcacagttt cagcgcttcc tggtagctga tccagtcatc ggttgtgacg ttgtacacgc
     1381 cgggcttgtt cttgcggaag gccagcgcca cagcgtggcc catgtcgtcc acgtggatga
     1441 actgcatcat gggggaaaag cccatcaaca cgggcgcgaa cttgctcgac agcagcagac
     1501 tcatggaatt cagcacgccc gggccggcga tgttgcaggg ccgcagaatc gtgatattca
     1561 gctccgggta tttccacaaa tagatgttgg ccaggttttc cagctccacc gaatccacca
     1621 ggtccatggt gagttccgat gccttcagcg gcgcctcttc tgtcagcagc gcggggttgt
     1681 aggggctggc gccgtatacg aagaacgtcg acagtaccac caccttgtgc acgccgtatt
     1741 tgtgactcaa atccagcaga cgctgcgtgc ccagcacgtt gtggttgtaa cggttgcggt
     1801 gggtggattc atacaggccc atccggccca ggtgaatcac gccttcgatc ttgtgcttgc
     1861 ggaaaatgtc ctcgaagccg cgcttgttgt attccacgcg gtagctggac atgccctctt
     1921 ccagcggcac acgacggcga aagtccacca gcaccacgtg atagtcgcgc ttgagctgct
     1981 ggatcacctg ctgggccagg gcaccgccag cacccgtgat caggatttcc ggtttctgct
     2041 tgtgaatcat ttgaaccacc cttcccgttc tttcaggccc cgcgcaatca gcttgttgat
     2101 ttcgtccttc acctgttcca ccttgatcgc cacgtcatcc tcggtggtga cggggccttc
     2161 aaaaagcatc ggcttgccaa aataaatgcg caccttggcc ggcagcggta tcggcgtggt
     2221 gaccggcaga tagggtaggc ccagcaggcg ccccagtgtt ttcaggtgga acggcgtggg
     2281 catggtttcc tcgcagccca ccacccccac cggcaggatc ggtgtgcgtt cggtgatcgc
     2341 caggtgcata aagcccaggc cgaagcgctg cagtttgtag cgtttgtccc aggtcttgcc
     2401 ggcgccacgc acgccttccg ggaacacgat gatggcttcc tcgctgcgca gcatcttctc
     2461 gcagttttcc gggtcgccca ccacaccgcc catctcgttc atgatgttgc cgatgaaggg
     2521 catcgtcggg aaccagcgct cgatcatcac gcgcagggaa cggggcccgt ggggattggt
     2581 ggccatggcg tagcccacca gggtgccgtc gatgggcagc tggccgctgt gattggcgat
     2641 caccagcaga cggcccgacg tgggcacgtt ctccagcccg aaggcttcga cgcggaagta
     2701 tttttcatag aagggtttca gcgccgccgt ggcgatcttg gtggtgtcgc tgttgtagcc
     2761 ccagggatcg taaccgaaag aaccgaccgg cttgggtatc cggttgatga tcgcttccgt
     2821 ttcagcggaa acgaaatgac gcttcagaaa gtctctgata ctcatgctga tgcaacctca
     2881 taaattccct tgaatgcacg tccgtcatgc tccctcaagc acggcggacg cacgaatccc
     2941 gagccacaat cgggctcata caaacaaaat aaaactgcaa catacggcct tatgcaatga
     3001 caatgcccta aatggaacat tgccatgatc tggcgcactg gaatgacaaa tgacggggaa
     3061 aggttcttgt tgttgtgggc gggtttcgtc catcccggac gatacagcac ctgcgagc
//
LOCUS       DASUEG010000744         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1643847, whole genome shotgun sequence.
ACCESSION   DASUEG010000744 DASUEG010000000
VERSION     DASUEG010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643847"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..544
                     /locus_tag="VFX11_14080"
     CDS             41..544
                     /locus_tag="VFX11_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939801.1"
                     /translation="MSTTALSSPILRPEDADFIRSGVSLCVASRDVRHVPSLARALGC
                     AVCDGGRTLRLLLVKSQCEALLRDLEKSGQIAVVFTRPSTHRSLQIKGSDARLETATP
                     GDEQLAQACFEAFADDLLTIGFSRAFTGLYYQHEPSDLVAVIFTPDDVFMQTPGPNAG
                     ARLEPLP"
     gene            541..1887
                     /locus_tag="VFX11_14085"
     CDS             541..1887
                     /locus_tag="VFX11_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4939802.1"
                     /translation="MKIALDTIRHSLEGAIPGVVATCDANGIPNVSYVSQVHYVDSNQ
                     VALSFQFFNKTRGNMLANPQLTAYLIDPETVARYRLALLYLRTETDGPLFQNMKAKLA
                     GIASHTGMAGVFELRGADVCRVLDIERVPGAEAPRALPPYNWLTVLRALGQRVAGCGD
                     MARLFEEVLGGLEEMLDIRHSLLLMWDECGHKLYTVASRGYVQSGIGSEIPLGCGVIG
                     VAAQYQTPIRITHMATEYSYSKAVRENAGLDPQRLETAIPFPGLAEPHSQMALPLLAS
                     GRLLGVLYVESEQDMRFGFEDEDGLSVLAQQIALTLVQLQQAADVETDTAAVAAGASP
                     AGTRLPVRFYPANHSVFIGDDYLIKGVAGAILWRLLHYYVHENRTEFSNRELRLDKHL
                     NLPDIDDNLEARLILLQRRLAEKSSALAIVKTGRGRFRLQVNQPVQLLEHEVGASF"
     gene            complement(1855..2283)
                     /locus_tag="VFX11_14090"
     CDS             complement(1855..2283)
                     /locus_tag="VFX11_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HEX4939803.1"
                     /translation="MEMKMENFAARWIYMAVIYFVVAVGLGIFMGASHDHSLMAVHAH
                     LNLLGWVSQGLTGVIYHYFQNAGRSKLASAHFWIYNVALAPMMLALALLLKGNVAAEP
                     VLGILSMVMGISILLFAINMFLPRKANDSASERRADLVLQ"
     gene            complement(2341..3009)
                     /locus_tag="VFX11_14095"
     CDS             complement(2341..3009)
                     /locus_tag="VFX11_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX4939804.1"
                     /translation="MHQQRFDIYRLIHKGLRAYLTETLLSVGRMDLEASADLHAGLTQ
                     LHSLLDFCESHLQHENTFIHPAMNLCQPGSADAMSEHHQQHVAMIAELRGAAHQLRAA
                     AAPDRELLAAQLYRKLALFVADNLAHMHEEETRNNAILWNCYSDAEIQAIHQAILQSI
                     PPQENAVGMHWMLAALNHQERHALLSGMRREAPQPVFENTLAIARQNLPRSEWNKLQF
                     ALAC"
     gene            3216..>3913
                     /locus_tag="VFX11_14100"
     CDS             3216..>3913
                     /locus_tag="VFX11_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX4939805.1"
                     /translation="MIRSILLTADGSLLRFDSSAAERWRDNPGSVLWLDLENEDPHAE
                     EKLLLDLNINPMAISDAQRKRHPPKVEEFDHYTFMLYRGFQQFDEDLRIDHIQVAFFV
                     GTHFLITRRDGNSIGIQSVWDQNNELTTYLKKPALLMMRILRASMNGYLGGLLEMEDK
                     LSQIEDDMLTRPTDTMMHKLVMYRSRLRKLNRVFSYHERIVRQIIKEPTAELNGEDVA
                     LYHALQDVYDKCERL"
BASE COUNT          755 a         1154 c         1201 g          803 t
ORIGIN      
        1 acataagatg aacctatctc caccgggata tccattgacc atgtccacca ccgccctctc
       61 atcccccatc ctgcgccccg aggacgccga cttcatccgc tccggcgtgt ccctctgcgt
      121 agcctcgcgg gatgtgcggc atgtgccgtc cctggcgcgt gcgctggggt gtgcggtgtg
      181 cgacggcggt cgtaccctgc gcctgctgct ggtgaaatcc cagtgcgagg cgctgctgcg
      241 ggatctggag aagtccggtc agatcgccgt ggtgttcacc cggcccagca cgcaccgctc
      301 gctgcagatc aagggctcgg acgcgcgtct ggaaacagcc acacccgggg acgagcagct
      361 ggcacaagcc tgtttcgagg cctttgccga cgacctgctc accatcggtt tttcccgcgc
      421 cttcaccggg ctctattacc agcatgagcc atccgatctg gtggccgtga tcttcacgcc
      481 ggacgacgtg ttcatgcaga cgcctggccc caatgccgga gcccggctgg agccgctgcc
      541 atgaaaatcg ccctggatac catccgtcac agcctggagg gcgcgatccc cggcgtggtc
      601 gccacctgcg acgccaacgg gattcccaat gtgtcctatg tgtcgcaggt gcattatgtg
      661 gacagcaatc aggtggcgct gtccttccag tttttcaaca agacccgcgg caacatgctg
      721 gccaatcccc agctcactgc gtatctgatc gatccggaaa ccgttgcgcg ctatcgcctg
      781 gcgctgctgt acctgcgcac cgaaaccgat ggcccgctgt tccagaacat gaaagcgaag
      841 ctggcgggaa ttgcctcgca cacgggcatg gccggcgtgt ttgaactgcg cggcgccgat
      901 gtgtgtcgcg tactggatat tgagcgtgtg cccggtgcgg aggcaccgcg cgcgttgcca
      961 ccctacaact ggctgaccgt gttgcgggcg ctgggccagc gcgtcgcggg ttgcggcgac
     1021 atggcgaggc tgttcgagga agtgctgggc gggctggagg aaatgctcga tatccgccat
     1081 tccctgctgc tgatgtggga cgagtgcggc cacaaacttt acacggtggc gagccggggt
     1141 tatgtccagt ccggcatcgg ttcggaaatt ccgctgggtt gcggcgtgat cggcgtggcg
     1201 gcgcagtacc agacgccgat ccgcatcacc catatggcga cggaatacag ttacagcaag
     1261 gcagtgcggg aaaatgcggg tcttgatccg cagcggctgg agacggcgat tccgtttccc
     1321 ggcctggcgg agccccacag ccagatggcg ttgcccttgc tggcgtcagg caggctgctg
     1381 ggcgtgctgt atgtggaaag tgaacaggac atgcgcttcg gtttcgagga tgaagacggc
     1441 ctgtcggtac tggcgcagca gattgcgctc acgcttgtgc aactgcagca ggcggccgac
     1501 gtggaaacgg ataccgctgc cgttgcggcg ggcgcatcgc cggcgggcac gcgtctgccg
     1561 gttcgctttt atccggccaa tcacagtgtc tttattggcg acgattatct gatcaagggc
     1621 gttgccggcg ccatcctctg gcgtctgctg cactactacg ttcatgaaaa ccgcaccgaa
     1681 ttcagcaacc gcgaattgcg gctggacaaa cacctgaacc tgccggatat cgacgacaac
     1741 ctggaagcgc gcctgattct gttgcagcgg cggctggcgg aaaaatccag cgccctggcg
     1801 attgtgaaga cagggcgtgg acgttttcgt ctgcaggtga accagccggt gcagttactg
     1861 gagcacgagg tcggcgcgtc gttctgatgc actgtcgttc gccttgcgcg gcaggaacat
     1921 gttgattgcg aacagcagga tgctgatgcc catcaccatc gacagaatgc ccagcaccgg
     1981 ctccgccgcc acattgccct tgaggagcaa tgccagcgcc agcatcatcg gcgccagcgc
     2041 cacgttgtag atccagaaat gcgccgacgc cagtttgctg cggcccgcgt tctggaagta
     2101 gtgatagatc acacctgtca gcccttgtga cacccagccc agcagattca ggtgcgcgtg
     2161 caccgccatc agactgtgat cgtgggatgc gcccatgaaa atgcccaggc ccacggccac
     2221 cacgaaataa atcactgcca tgtagatcca gcgtgcagca aagttttcca ttttcatctc
     2281 catgataatc tccttgtttg gcaattaaaa aagttggtga tcaaatactg gttccgcgtt
     2341 tcagcaggcc agcgcgaact gcagtttgtt ccattcggaa cggggcaggt tttgccgcgc
     2401 aatcgccagc gtgttctcga acaccggttg cggcgcttcc cggcgcatgc cgctcagcag
     2461 cgcatgccgt tcctgatgat tcagcgctgc cagcatccag tgcatgccca ctgcattttc
     2521 ctgcggtgga atggattgca ggatcgcctg atggatggcc tggatctcgg cgtcgctgta
     2581 gcagttccac aaaatggcgt tgttgcgggt ttcctcttcg tgcatatgcg ccaggttgtc
     2641 tgccacgaac agcgccagtt tgcggtacag ctgggccgcc agcagttcac ggtctggcgc
     2701 ggcggctgcg cgcagttggt gtgccgcgcc gcgcagttcc gcgatcatcg ccacatgctg
     2761 ctggtgatgt tccgacatgg cgtcggccga cccgggctgg cacaggttca tggcagggtg
     2821 gataaaggtg ttttcgtgct gcagatggct ttcgcagaaa tccagcaggc tgtgcaactg
     2881 ggtcaggccc gcgtgcagat cggcggaggc ttccaggtcc atgcggccga cactgagcag
     2941 ggtttccgtc agataggcgc gcagaccttt gtgaatgagc cggtaaatgt cgaagcgttg
     3001 ctggtgcatg gtggtctctc cctggtgttc ggtgatggaa agcatatgcg gggaagacgc
     3061 gcggcgttgc taaatgttgc gtaagaaaac cctaacattt ccttaagttt tggccgtttt
     3121 cagcggtggc aaaagcagtg ccggaatgat gtgaaaatta ccgattagtc gctacaatgt
     3181 tgcgcactac ggggcccgca caggaagcag gacgcatgat ccgatccatt ctattgaccg
     3241 ccgatggcag tcttctccgg tttgattcgt ccgccgccga gcgctggcgc gacaacccgg
     3301 gcagcgtgct ctggctggat cttgagaacg aagatcccca cgccgaggag aaactgctgc
     3361 tggacctgaa catcaatccc atggcgatca gtgacgccca gcgcaagcgc catccgccca
     3421 aggtggagga attcgatcac tacaccttca tgctgtatcg cgggtttcag cagttcgacg
     3481 aagatctgcg gatcgaccac attcaagtgg cgttttttgt tggcacccat ttcctgatca
     3541 cccgtcgcga tggcaattcc atcggcatcc agtcagtgtg ggatcagaac aatgaactca
     3601 ccacctacct gaaaaagccg gcattgctga tgatgcgcat tctgcgcgca tccatgaacg
     3661 gctatctggg cggactgctg gagatggagg acaagctgag ccagatcgag gatgacatgc
     3721 tgacgcgccc caccgatacc atgatgcaca agctggtcat gtaccggtcg cgcctgcgta
     3781 aactgaaccg ggtgttcagc tatcacgaac ggatcgtgcg ccagatcatc aaggagccga
     3841 cagcggaact gaacggcgag gacgtggcgc tgtaccacgc actgcaggat gtgtacgaca
     3901 agtgtgagcg cct
//
LOCUS       DASUEG010000745         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_639274, whole genome shotgun sequence.
ACCESSION   DASUEG010000745 DASUEG010000000
VERSION     DASUEG010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639274"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFX11_14105"
     CDS             complement(<1..971)
                     /locus_tag="VFX11_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939806.1"
                     /translation="MEQARGEFIPREELERRLTALGERNLKALPLFAARCAMRAFPLL
                     GVPEDFDFWPEQERTLNLLAVWRALMLALSGEWMPSQAVTGARRAAAAAQGFLAGGEG
                     SALRGEVLKWSTLVAVAAQVAQAAAVPALSAVEQVALAIGVAQTVHPFLVHLAAEDTT
                     AVVLQELDQWESRGADIVQASPLWLGLTPSSRFVELAFERLPQQLQQRAKRESRQDSL
                     NIRLITGIADRYRALLERGYPVPEAQQDLSALNRYFDSPAATSGTAPSTTVTASAKVA
                     QEPWDETQVVKAIPDLETLASAVPGKIPLDETLFQEFPPKAPAPAESA"
     gene            complement(1093..2268)
                     /locus_tag="VFX11_14110"
     CDS             complement(1093..2268)
                     /locus_tag="VFX11_14110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939807.1"
                     /translation="MNALILWLEDQFRLQRDINGCLVMSILPLPLFLLFLTIPWVAQT
                     DPAWSAIYNRDLLPFTQIMLGLVILCQLGIGAASWVNRHCGADLPVLSLFTVVTVFAG
                     LITLCLGYGYKDSPLMLLCMGMLILTRALFKPPVYKAVFVTMGVLFVFSEIAFWSNAF
                     PYAPLLRSPIFLGDQLTPWWSFWLRVVYCMIAIPMVALFFLFAHIMEREKQLLEQLVR
                     TDSLTGLANRREFMQRFQVELHRHARRHKPLCLLLIDVDHFKQVNDNHGHPAGDRVLE
                     QLGAILRNTLRRNVDIAARFGGEEFVVLLPETALPQARAVAEHLARTLEAEEFRATQG
                     TFNVTLSGGLVQISGTDPDRALRVADENLYAAKQSGRNRVVASVMTREELLPAVVCE"
     gene            complement(2243..>3042)
                     /locus_tag="VFX11_14115"
     CDS             complement(2243..>3042)
                     /locus_tag="VFX11_14115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4939808.1"
                     /translation="GLLLLDMRSTGIALGLGVIFIWLNDLAIFSGLLPYAPGYAPAAF
                     TDGKHALMAEAFRTGVLYVSVAAYGLLIWVLFGHYDFHRHSLTLMSNQDMLTGLANRR
                     HFMERLEAECARLTRIHAPLCLVMIDADHFKKINDSHGHPVGDDVLRTLSHLLGQHMR
                     VPADLPARIGGEEFAMLLPDTRLEGAVAVCERIASSLRRTPLQSPQGPFHITLSMGVV
                     EGWQLDPEKLLHYADANLYLAKAQGRNRIIASRISPEAVDERADTLA"
BASE COUNT          638 a         1001 c          885 g          518 t
ORIGIN      
        1 gccgattcag cgggagcagg cgctttgggt ggaaattcct gaaacagggt ttcatccagc
       61 ggaatttttc cgggcaccgc agacgcgagc gtttccagat ccggaatggc cttgaccacc
      121 tgggtttcgt cccagggctc ctgcgccacc tttgctgacg ccgtaaccgt ggttgaaggc
      181 gctgtaccgg acgtcgcagc agggctgtcg aaataccgat tgagtgcgga aagatcctgc
      241 tgggcctcgg gcaccgggta accgcgttcc agcaacgccc ggtagcggtc tgcaattccc
      301 gttatcaatc ggatattcag ggaatcctgc cgcgattcgc gtttggcccg ctgctgcaac
      361 tgttgcggca ggcgttcaaa tgccagttcc acaaaacggg aagaaggggt aaggcccagc
      421 cagagtggcg aagcctgcac gatgtccgcg cccctggatt cccactgatc cagctcctgc
      481 aaaacgacgg cagtggtgtc ttccgcggcc agatgaacaa gaaacggatg caccgtctgt
      541 gcaaccccga tggccagcgc cacctgctcc acggcggaca aagcgggcac agcggctgcc
      601 tgcgcaacct gcgccgccac cgctaccagg gtggaccatt tcagcacttc cccccgcagc
      661 gccgaacctt ccccgccggc cagaaaacct tgcgctgctg ccgccgcgcg ccgggcaccg
      721 gtcactgcct gggacggcat ccattcgccc gacaaggcca gcatgagggc acgccacacg
      781 gccagcaggt tgagcgtccg ctcctgctcc ggccagaaat caaaatcctc cggcaccccc
      841 agcaggggaa atgcgcgcat agcgcagcgt gccgcaaaca acggcagcgc cttcagattc
      901 cgttccccca acgctgtcag ccggcgttcc agctcttccc gtggaatgaa ttcaccgcgc
      961 gcctgctcca ttgtccatgc acctctccat gcacgtctcc gtgcacgctg accgggcggc
     1021 gacgttccac tctgcgtcac gcccggttcc ggttcaatcc tactgcatcc gttccattat
     1081 gagccgaaac cctcattcac acacgactgc cggcagaagc tcctcccgtg tcatcaccga
     1141 tgccaccacc ctgttgcggc cggactgctt ggcggcatac aggttttcgt ccgccacccg
     1201 cagggcccgg tccgggtcag taccgctgat ctgcaccagt cccccactca gggtgacgtt
     1261 gaacgttccc tgcgtcgccc ggaattcctc tgcctccagc gtgcgcgcca ggtgttccgc
     1321 cacggccctg gcctggggca gcgcagtttc cggcagcagc accacgaact cctccccgcc
     1381 aaagcgcgcc gcgatatcga cgttgcgacg cagggtgttg cgcaggatgg cccccagctg
     1441 ctccagcacc cggtcgccgg caggatggcc gtggttatcg ttgacctgct tgaaatgatc
     1501 cacatcgatc agcagcagac acaggggttt atgccggcgg gcatggcgat gcagttccac
     1561 ctggaagcgc tgcatgaact cgcgacgatt ggccagaccg gtgagggaat cggtgcgcac
     1621 cagttgctcc agcagctgtt tttcccgctc catgatatgc gcgaacagga agaacagcgc
     1681 caccatggga atcgcgatca tgcagtacac cacacgcagc cagaacgacc accagggcgt
     1741 aagctgatca ccgagaaata tcggggaccg cagcaggggc gcgtagggaa aggcattgct
     1801 ccagaaggct atttcgctga acacgaacag cacccccatg gtgacgaaca cagccttgta
     1861 cacgggcggt ttgaacagcg cgcgggtcag gatgagcatg cccatgcaca acagcatcag
     1921 cggcgaatcc ttgtaaccgt atcccaggca cagtgtgatc aggccggcaa atacagttac
     1981 gacggtgaac agcgacaaca ccggcagatc agcgccgcaa tgccgattga cccagctggc
     2041 ggcaccaatg cccaactggc acaggataac cagccccagc atgatctggg tgaacggcag
     2101 cagatcacga ttgtaaattg cactccaggc tggatcggtc tgggccaccc agggaatggt
     2161 caggaacagc agaaacagcg gcaacggcag aatcgacatc accaggcagc cattgatgtc
     2221 ccgctgcagg cgaaactggt cctcaagcca gagtatcagc gcgttcatcg acggcctccg
     2281 gtgatatgcg ggatgcgatg atgcggttgc ggccctgcgc cttggccagg tacagattgg
     2341 catcggcata gtgcagcagt ttttccgggt ccagttgcca gccttccacc acccccatgc
     2401 tcagcgtgat atggaacggc ccctgcggac tttgcagcgg cgtgcgacgc agactggagg
     2461 caatacgctc acacacggcc acggcgcctt ccagccgggt gtccggcagc agcatggcga
     2521 actcctcgcc accgatccgc gccggcaggt ccgccggcac ccgcatgtgc tggcccagca
     2581 gatgtgacag ggtgcggagc acatcgtccc ccaccggatg tccatggctg tcattgattt
     2641 tcttgaaatg gtcagcatcg atcatgacca gacacagtgg tgcgtgaatc cgcgtgagcc
     2701 gggcacactc ggcctccagc cgctccatga aatgacggcg gttcgccagc ccggtgagca
     2761 tgtcctggtt cgacatcagg gtcagcgaat gccggtgaaa atcgtagtgt ccgaacaaca
     2821 cccagatcag caagccgtag gcggcaacac tcacgtacag gacaccagtg cgaaaggctt
     2881 ctgccatcag cgcatgcttg ccgtcagtga aagccgctgg cgcgtagccg ggtgcatagg
     2941 gcagcaggcc actgaaaatc gccagatcgt tcagccagat gaagatcacg cccagcccca
     3001 gtgcaatgcc agtggagcgc atgtccagca acaacaggcc gc
//
LOCUS       DASUEG010000746         6195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_340211, whole genome shotgun sequence.
ACCESSION   DASUEG010000746 DASUEG010000000
VERSION     DASUEG010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340211"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="ribB"
                     /locus_tag="VFX11_14120"
     CDS             complement(<1..1018)
                     /gene="ribB"
                     /locus_tag="VFX11_14120"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX4939809.1"
                     /translation="MQLNTIEEIVEDIRHGKMVILMDDEDRENEGDLVMAASMVRPED
                     INFMIRNARGLVCLTMTRERCQQLNLPLMVSGANGSQFGTNFTLSIEAAEGVTTGISA
                     ADRARTVHAAVARDAKPSDIVQPGHIFPIMAQPGGVLRRAGHTEAGCDLARMAGLEPS
                     SVIVEIINEDGTMARRPDLEKFAEAHGLKIGTIADLIHYRTVNEKTIELVRSEKFPTH
                     YGDFQLHLFRDLIENRTHVALVKGEMASGKPPFVRVHVADPLRDLLHAVKADSSGWSL
                     SRSLQYLSEHDEPGVILLLAESFDSHNMDELVDSFYGGRPSKRQLARDGTSVYRTIGT
                     GSQIL"
     gene            complement(1197..1859)
                     /locus_tag="VFX11_14125"
     CDS             complement(1197..1859)
                     /locus_tag="VFX11_14125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009877041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX4939810.1"
                     /translation="MFTGIIEAVGKIARMEPKGGDMRLRIQTGKLGLADVKLGDSIAV
                     NGVCLTVVELPGDGFWADVSLETLNHTSFKQLKTGSPVNLEKALTPQTRLGGHLVSGH
                     VDGLGEIVSRHEDARSIHFKVKAPDALAKYIAHKGSICVDGISLTVNAVNGAMFDLNI
                     VPHTVLETTVGQWQPGVQVNLEVDVIARYLERLLLGEKAADPKQEGGITLGFLAENGF
                     LK"
     gene            complement(1863..3026)
                     /gene="ribD"
                     /locus_tag="VFX11_14130"
     CDS             complement(1863..3026)
                     /gene="ribD"
                     /locus_tag="VFX11_14130"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX4939811.1"
                     /translation="MSPPLFNAQDHQHMADAIRLAGRGLYTTHPNPRVGCVIVKDGVV
                     VGQGWHAIAGQGHAEVNALRAAGPAAQGATVYVSLEPCSHHGKTPPCADALIQAGVAR
                     VISAMEDPNPKVSGSGHQRLQAAGIETACGLLEAQARAINPGFYKRMQTGLPWVFLKS
                     AMSVDGRVAMASGESQWISCGNSRADVQRLRARVEAIVTGVDTVLQDNPALTVRPELW
                     PAPVAGDEPLSGWSWPVDFAPLQPLRVILDSRLRTPPDAAILRQPGKTLIACIDRDEA
                     KARLLEQAGAEILLLPARAGRVDLPSLLQELARREVNEVLVETGGQLAGSFVNEQLVD
                     EWICYLAPKLMGSSARPVLALAGINTMSQAHDLQLTDLRQIGQDIRMTYRWSR"
     gene            complement(3102..4031)
                     /locus_tag="VFX11_14135"
     CDS             complement(3102..4031)
                     /locus_tag="VFX11_14135"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX4939812.1"
                     /translation="MKPDDQLIFRPHNQLAALARSGAPVHLFVNPVEYHGPHLSLGND
                     RILSERIALKLHERLLQKPDEFPFVSGGHIDMGCDPCPGPGSVSTTYSQLKSSLRQRV
                     DALVDIGVQRVIFHTFHGSPFHQHALDWAVERLRRKGVRAVNLFSATIDLIVNFDPAL
                     YAPLRDLLPSPADFERVLAILPSDHHAGFFETSVALYVAPETVAPDYRSVPPCPPLPA
                     SAALMGLARVLKSRELENTAYSMAWMLLKPFPGYTGIPALASPQAGRFYVEDLILPRY
                     QQRCRDVLWGDAPPPRAGFAWTRPFAPIVGGRI"
     gene            complement(4028..4513)
                     /gene="nrdR"
                     /locus_tag="VFX11_14140"
     CDS             complement(4028..4513)
                     /gene="nrdR"
                     /locus_tag="VFX11_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX4939813.1"
                     /translation="MHCPFCNADDTKVIDSRLVAEGNQVRRRRECTACGERFTTYESA
                     ELLMPKVVKSDGTREPFDEHKLRGGILKALEKRPVSVEQIEAAISKICHKLRATGERE
                     LSSRVLGEEVMDALRELDQVAYVRFASVYRDFQDLSEFSDEIARLQKTGDGKNEGKNA
                     K"
     gene            complement(4642..5106)
                     /locus_tag="VFX11_14145"
     CDS             complement(4642..5106)
                     /locus_tag="VFX11_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4939814.1"
                     /translation="MDRYDQRILEELQQDGRVSNQDLADRIGLSPSPCLRRVRQLEED
                     GIIDRYVALLRPEKLGLKLTALIQISMDKHTPERFENFERQVKSFPEVQSCYLITGQS
                     ADYILRVVVPDMERYQEFLLGKLTRIDGVSGVHSSFVMRKVVDSTAVPLNAG"
     gene            5433..>6195
                     /locus_tag="VFX11_14150"
     CDS             5433..>6195
                     /locus_tag="VFX11_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX4939815.1"
                     /translation="MAFDFRKYRPYLPVQLRDRTWPDQTIDTAPAWCSVDLRDGNQAL
                     IDPMNVEEKKRLFALLVKVGFKEIEVGFPAASQPDFDFVRALIEENRIPDDVTIQVLT
                     QSRDELIERSFESLRGAKKAIVHLYNSTSPTQREQVFGLDKTGITAIAVHGAQKVKSE
                     ARKYPQTQWSFQYSPESYSGTEVDYAVEVCNAVLDVWQPTAQNKVILNLPATVEMTTP
                     NVYADQIEYFCRHVNFRENVIISLHTHNDRGCAVAA"
BASE COUNT         1208 a         1989 c         1857 g         1141 t
ORIGIN      
        1 gcaagatctg cgaaccggtg ccgatggtgc ggtacacact ggtgccatcg cgggccagct
       61 gccgcttgga cgggcggccg ccataaaaac tgtccaccag ctcatccatg ttgtgactgt
      121 cgaacgattc cgccagcagc aggatcacgc cgggctcatc gtgctcggac agatattgca
      181 gcgagcgcga cagactccag ccgctggaat ccgctttcac cgcatgcaac agatcgcgca
      241 gcggatcggc cacatgcacg cgcacaaacg gtggcttgcc actggccatt tcgcccttca
      301 ccagggcaac atgggtgcgg ttttcaatca ggtcgcggaa caggtgcagc tggaaatcgc
      361 cgtagtgcgt ggggaatttt tcactgcgca ccagctcgat ggttttttcg ttcaccgtgc
      421 ggtagtggat caggtcggcg atggtgccga ttttcaggcc gtgcgcctcg gcgaattttt
      481 ccagatccgg acggcgcgcc atggtgccat cctcgttgat gatttccaca atcaccgatg
      541 acggctccag cccggccatg cgcgccagat cgcagccagc ttcggtgtgg ccggcacgcc
      601 gcagcacgcc gccgggctgc gccatgatcg ggaagatgtg acccggctgc acgatgtcgg
      661 acggcttggc gtcgcgcgcc accgccgcat gcacggtgcg ggcgcggtca gcggcggaaa
      721 tgccggtggt gacgccctcg gccgcttcga tcgacagcgt gaaattggtg ccgaactgcg
      781 aaccgttggc gccgctcacc atcagtggca ggttcagttg ctggcagcgt tcgcgggtca
      841 tggtgagaca caccagaccg cgcgcattgc ggatcatgaa attgatgtct tccggccgca
      901 ccatgctggc cgccatcacc agatcgcctt cattttcgcg atcctcgtca tccatcagga
      961 tgaccatctt gccgtggcga atgtcttcaa caatttcttc gatggtattc agttgcatgt
     1021 tcgttgctac ctgtttcgaa atttgcgccg aacgaggttg ggcgcgtctg gtgttccagt
     1081 tcgggacgct ctgcgacagg gatgtcgcgg agcagccccc agggacgggt tcacggcgtg
     1141 tcccgaaccg gaacaccaga cgtgaacaac cgtcactacg gagcacagtg aaatacctat
     1201 ttcaaaaacc cgttctctgc cagaaatccc agcgtaatcc caccttcctg cttcggatca
     1261 gccgccttct cacccaataa tagccgttcc agatagcggg caatcacgtc cacctccaga
     1321 ttcacctgca cccccggctg ccactgaccc accgtggtct ccagcacagt atggggcacg
     1381 atgttcagat caaacatggc gccattgacg gcattcaccg tcagactgat cccgtccaca
     1441 cagatggaac ccttgtgggc aatgtatttc gccagcgcgt ccggcgcctt caccttgaaa
     1501 tggatcgagc gcgcatcctc gtggcgggac acaatttctc ccagcccgtc cacatggccg
     1561 gacaccagat gcccacccag ccgggtctgc ggcgtcagtg ccttttccag attcaccggc
     1621 gaaccggttt tcagctgttt gaagctggtg tgattcagcg tttccagtga cacgtcggcc
     1681 cagaagccgt cgccgggcag ctcaaccacg gtgaggcaga cgccattcac cgcaatgctg
     1741 tcgcccagtt tgacgtcggc caggcccagc ttgccggtct ggatgcgcag gcgcatgtcg
     1801 ccgcccttgg gttccatgcg ggcgatcttg cccactgctt cgatgatgcc ggtaaacatg
     1861 attcagcgac tccagcgata cgtcatgcgg atgtcctggc cgatctggcg caggtcggtg
     1921 agttgcaggt cgtgggcctg gctcatggtg ttgatgccgg ccagcgccag caccgggcgc
     1981 gcactgctgc ccatcagttt gggggccagg tagcagatcc actcgtccac cagctgttcg
     2041 ttgacgaagc tgccggccag ctggccaccg gtctccacca gcacttcatt cacttcgcgc
     2101 cgggccagtt cctgcaacag cgacggcaga tccacccgcc cagcccgcgc gggaagcaac
     2161 aggatttccg caccggcctg ctccagcagt cgggctttgg cttcgtcgcg gtcgatgcag
     2221 gcgatcagcg ttttgccggg ctggcgcagg atcgcggcgt ccggcggtgt gcgcaaacgg
     2281 gaatccagga tcacccgcag tggctgcagg ggtgcaaagt ccaccggcca ggaccagccc
     2341 gacagcggct catcaccagc gaccggagcg ggccacaatt cgggccgtac cgtcagcgcc
     2401 gggttgtcct gcagcaccgt gtccacgccg gtgacaatgg cttccacccg cgcccgcagg
     2461 cgctggacgt cggcgcggga gttgccgcag ctgatccact ggctttcgcc gctggccatg
     2521 gcaacgcggc cgtccacgct catggcggat ttcaggaaca cccagggcag gccggtctgc
     2581 atgcgcttgt agaagccggg attgatggca cgggcctggg cttccagcaa accgcaggcg
     2641 gtttcaatac cggcggcctg caaccgctgg tggccgctgc cggatacttt gggattgggg
     2701 tcttccatgg cgctgatgac ccgggccacg ccggcctgga tcagggcgtc ggcgcagggc
     2761 ggggtcttgc catgatggct gcagggttca agggaaacgt aaacggtggc gccctgtgcg
     2821 gcaggcccgg cggcccgcag ggcgttgact tcggcatggc cctggccggc gatcgcatgc
     2881 cagccctgtc ccaccaccac gccgtccttc acgatcacgc agcccacccg cggattggga
     2941 tgagtggtat acaggccccg cccggccaga cgaatcgcgt cggccatgtg ctggtgatcc
     3001 tgggcgttaa agagcggtgg ggacatgaat ggagccaatg ccagtaaaaa aggaggggca
     3061 gtatatcaca ggggcagggc tatcagatcc gccagatccg ctcagatccg cccgcccacg
     3121 atgggcgcaa acggccgggt ccaggcaaag ccggcccggg gcgggggcgc atcgccccac
     3181 agcacatccc ggcagcgctg ctgatagcgg ggcaggatca ggtcttccac atagaaacgt
     3241 cccgcttgcg gcgacgccag ggcgggaatg ccggtatagc cgggaaaggg tttgagcagc
     3301 atccaggcca tggaataggc cgtattttcc agctcgcggg atttcagcac gcgggccagc
     3361 cccatcaatg cggcactggc gggcaacggc ggacagggcg gcacactgcg ataatccggt
     3421 gccacggttt ccggtgccac atacagggcc accgaggttt caaaaaaacc ggcgtgatga
     3481 tcgctgggca gaatcgccag cacccgctcg aaatccgccg gcgacggcag cagatcccgc
     3541 agcggcgcat acaacgcagg gtcgaaattc acgatcaggt cgatggtggc actgaacagg
     3601 ttcaccgccc gcacgccctt gcgccgtaac cgttccaccg cccagtccag cgcatgctgg
     3661 tgaaacggcg agccgtgaaa cgtatggaaa atgacgcgct gcacgccgat gtccaccagc
     3721 gcatccaccc gctggcgcag gctcgacttc agctgggaat aggtggtgct gacactgccg
     3781 ggccccggac agggatcgca acccatgtcg atatggccgc cggacacaaa gggaaattca
     3841 tccggcttct gcagcagacg ctcatgcagt ttcagcgcaa tccgctcgct gagaatgcgg
     3901 tcgttgccca gggacagatg ggggccgtga tactccaccg gattgacgaa cagatgcacc
     3961 ggcgcgccgg agcgggccag cgctgccagc tggttgtggg gcctgaaaat cagctggtcg
     4021 tctggcttca tttggcgttc ttcccttcat tcttgccatc gccagttttc tgcaggcggg
     4081 cgatttcatc ggaaaattcg ctcagatcct ggaaatcccg gtacacggac gcaaaccgca
     4141 cataggccac ctgatccagt tcgcgcaggg catccatcac ttcctccccc agcacccgcg
     4201 acgacagctc ccgctcgccg gtagcccgca gcttgtggca gatcttgctg atggcggctt
     4261 cgatctgctc cacgctcacc gggcgttttt ccagggcttt cagaatgccg ccgcgcagct
     4321 tgtgctcgtc gaatggctcg cgggtaccgt cagacttcac cactttcggc atcagcagtt
     4381 cagcggattc ataggtcgta aagcgctcgc cgcacgccgt gcattcgcgc cgccgccgca
     4441 cctgattgcc ctcggccacc agccgggagt cgataacctt ggtgtcgtca gcgttgcaga
     4501 acgggcagtg catgcggagc tccagcggtg aaaataatgg tgagtgtgaa cgaaatggct
     4561 ggcggaatca tacctgaaca ggcctggaat tcagcaggtt tcgcgcagtg tccgcaaatt
     4621 ggccgcgcat attgccgatg gctagccggc attcagcggt acagcggtcg aatccaccac
     4681 cttgcgcatg acgaaactgg aatgcacacc ggacacgccg tcaatacggg tcagcttgcc
     4741 cagcagaaat tcctggtagc gctccatgtc cggcaccacc acgcgcagga tgtagtcggc
     4801 actctggccg gtgatcaggt aacaggactg cacttcgggg aaacttttca cctggcgctc
     4861 gaaattctcg aaccgctccg gcgtgtgctt gtccatcgag atctggatca gcgcggtgag
     4921 tttcaggccc agtttttccg gccgcagcaa ggccacatag cggtcgatga tgccgtcctc
     4981 ctccagctgg cgcacccggc gcaggcaggg ggacggcgac aggccgatgc ggtccgccag
     5041 atcctggttg ctgacacggc catcctgctg cagttcttcc agaatgcgtt ggtcgtaacg
     5101 gtccacgggc tgcctcctgc cggggggatc ggctccacaa ttcgtgtttc tatgccggat
     5161 tatttctcaa atgctgactt aaaagcaata taattgcgca taaagccaaa atcatcgcca
     5221 cattcgcaat ccaatttccc ggccggcggc atagaattcc attccatcaa ggcacggtct
     5281 gcgccgctgc ggatatcccg ccccacccgg gcgccccact ccgccacggc aacccgccca
     5341 caaggcaggt caggcaacgg caacccaccc ggcccggacc caccttttcc caactgtcac
     5401 cacccaatgg acaccttcag ctggagcaca acatggcgtt cgacttccgc aaataccgcc
     5461 cttatctgcc cgtgcagttg cgggaccgca cctggcccga ccagaccatc gacaccgcgc
     5521 ccgcctggtg cagcgttgac ctgcgtgacg gcaaccaggc gctgatcgac ccgatgaacg
     5581 tggaagaaaa gaagcgcctg ttcgccctgc tggtgaaagt gggtttcaag gaaatcgaag
     5641 tgggcttccc tgccgcatcg cagccggatt ttgatttcgt acgcgcgctg atcgaggaaa
     5701 accgcattcc cgatgacgtc accatccagg tgctgaccca gtcccgcgac gaactgatcg
     5761 agcgcagttt cgaatccctg cgcggcgcca aaaaggcgat cgtgcatctg tacaactcca
     5821 cctcgcccac ccagcgcgag caggtgttcg gcctggacaa gaccggcatc accgccatcg
     5881 cggtacatgg cgcgcagaaa gtaaaaagcg aggcgcggaa atatccgcaa acgcagtggt
     5941 cgttccagta ttcgccggaa agttacagcg gcaccgaggt ggattacgca gtggaagtct
     6001 gcaacgccgt gctcgacgtg tggcagccga ccgcgcagaa caaggtgatc ctgaatctgc
     6061 ccgccaccgt ggagatgacc acgcccaatg tgtacgccga ccagatcgaa tacttctgcc
     6121 gccacgtgaa ttttcgtgaa aatgtgatca tcagcctgca cacccacaat gatcgcggtt
     6181 gcgcggtggc ggcag
//
LOCUS       DASUEG010000747         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_546643, whole genome shotgun sequence.
ACCESSION   DASUEG010000747 DASUEG010000000
VERSION     DASUEG010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546643"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFX11_14155"
     CDS             complement(<1..1115)
                     /locus_tag="VFX11_14155"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4939816.1"
                     /translation="MSSDPVVIASYARTPMGGMLGNFASVKATDLGAAAVKAALERAG
                     IAGDVVDRIYMGCVLPAGLGQAPARQAALAAGLPRSVQATTVNKMCGSGMQAAILATE
                     TLLAGGADVIIAGGMESMSNAPYLLSKHRAGSRIGHDTVKDSMYLDGLEDAYEEGTLM
                     GVFAENNARKFGFTRADQDAYAVEGLARAKLAIANGLFEEEIVPVEVKTKTGMQRVAV
                     DEQPGKANPEKIPSLKPAFAKDGTITPANSSSISDGAAALVLTRASVAKRLGMKVCAR
                     IVATGCHAHEPGDFPSAPVPAMKKVMAQAGWSVDDVDLFEVNEAFAVVAMIAMKELSI
                     PHEKINVNGGACALGHPIGASGARIVATLLAALKNRG"
     gene            complement(1160..2224)
                     /locus_tag="VFX11_14160"
     CDS             complement(1160..2224)
                     /locus_tag="VFX11_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939817.1"
                     /translation="MRPQTQRFEAAGGYPPELFVELAGLGLMGMTAPEEFGGSGADYV
                     SYALALMEIAAADGALSTILSIQNSLIVSALLKDGSAEQRQRFLPDLISGRKIGAFAL
                     TEADAGSDASAIRTRATKNGNNYVLNGSKQFITSGKIAGLAIVFAVTDPAAGKKGITG
                     FLVPTDRDGYKVDKVEHKLGQGASDTCGLRFENLQIEPELRLGQEGQGYRIALSNLEF
                     GRIGIAAQSIGMAQAALDIAVAYAKDRHSMGKAIIDHQAVGFRLADLAARLEAARQLV
                     LHAASVKDTGTPCLKEASMAKLVASETAEAVCSGAIQTLGGYGYLEEFGLAKIYRDVR
                     VCQIYEGTSDIQRMVIARSL"
BASE COUNT          434 a          674 c          658 g          460 t
ORIGIN      
        1 aaaccacggt ttttcagcgc ggccaacagg gtggccacaa tccgcgcacc ggatgcaccg
       61 atgggatggc caagggcgca ggcgccaccg ttgacgttga tcttttcgtg gggaatggac
      121 agttctttca tggcaatcat cgcgaccacc gcgaatgctt cgtttacctc gaacaggtcc
      181 acgtcgtcaa cggaccagcc cgcctgcgcc atcacttttt tcatggccgg aaccggcgcg
      241 ctgggaaaat cccccggctc atgggcatgg caacctgtcg ccacgatgcg ggcgcacacc
      301 ttcatgccca ggcgtttggc tacgctggca cgggtgagaa cgagagcggc ggcgccatca
      361 gatatcgacg acgaattcgc aggtgtaatg gttccgtcct tcgcaaatgc aggtttcagg
      421 gacgggattt tttccggatt ggctttgccg ggctgttcat ccacagcgac ccgctgcatg
      481 ccggttttgg ttttcacttc aaccggtacg atttcctctt caaacagtcc gttggcaatc
      541 gccagcttgg cgcgtgcgag tccttccacc gcatacgcat cctgatccgc ccgggtgaag
      601 ccaaacttgc gtgcgttgtt ctcggcgaaa acgcccatca gggtgccttc ttcgtaggcg
      661 tcttccagac catccaggta catggaatct ttcaccgtgt cgtgaccgat gcggctgccg
      721 gcccggtgtt tggacagcag atagggcgcg ttggacatgg actccatgcc gccggcgata
      781 atgacatcgg cgccaccggc cagcagcgtt tctgtcgcca gaatggcggc ctgcatgccg
      841 gaaccacaca tcttgttgac ggtagtggcc tgcaccgagc gcggcaatcc ggccgccagc
      901 gccgcctgac gtgcaggtgc ctgacccaga cccgctggca acacgcatcc catataaatg
      961 cggtcgacaa catcaccggc aattccggcg cgttcgagtg cagccttgac cgccgccgcg
     1021 cccaggtcgg tggcttttac cgaggcaaaa ttgcccagca ttccgcccat gggtgtgcgg
     1081 gcataggaag caatgactac aggatctgat gacatgttga attccttgct aagtaggaaa
     1141 ttggtgatac gaatcggggt taaagcgacc gggcgatgac catgcgctgg atgtccgagg
     1201 tgccctcgta aatctggcat acgcgcacat cacggtagat tttggccagt ccgaattctt
     1261 ccagatagcc gtacccaccc agggtctgaa tggcgccaga gcagacggct tctgccgttt
     1321 ccgacgcgac cagttttgcc atggacgctt ccttgaggca aggcgtcccc gtgtccttga
     1381 cgctggccgc atgcagcacc agctgacgcg ccgcttccag acgggccgcc agatccgcca
     1441 gacggaatcc aacggcctga tgatcgatga tggccttgcc catggagtga cggtctttgg
     1501 cataggcaac ggcaatatcc agcgccgcct gcgccatgcc gatggattgg gccgcaatgc
     1561 cgatgcgacc gaattccaga ttggacagtg caatgcgata gccctggcct tcctgaccta
     1621 gccgcaactc aggttcgatc tgcagatttt caaagcgcag gccgcaggtg tcggaggcgc
     1681 cctgtccgag tttgtgctcc accttgtcca ccttgtagcc gtcacgatcg gtgggcacca
     1741 ggaatccggt aatgcccttc ttgccggcag cggggtcagt caccgcaaac acgatggcca
     1801 gtccggcgat tttgccggag gtaataaact gcttggaacc attcaacacg tagttgttgc
     1861 cgtttttcgt cgcccgggtg cggatcgcgg atgcgtcgga acctgcatcc gcttccgtca
     1921 gggcaaaggc gccgatcttg cggccgctga tgaggtccgg caggaaacgc tggcgttgtt
     1981 ccgcgctgcc gtctttgagc agggcggaca caatcaggct gttctggatg gaaaggatag
     2041 tggaaagcgc gccatcggcc gctgcaattt ccattaatgc cagcgcgtag gaaacataat
     2101 ccgcgccgga accgccaaat tcttccggtg cggtcatgcc catcaggccc agtccagcca
     2161 gctctacaaa cagttcaggt gggtagccgc cggcagcttc gaagcgttgg gtttgagggc
     2221 gcagct
//
LOCUS       DASUEG010000748         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1511259, whole genome shotgun sequence.
ACCESSION   DASUEG010000748 DASUEG010000000
VERSION     DASUEG010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511259"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VFX11_14165"
     CDS             complement(<1..158)
                     /locus_tag="VFX11_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX4939818.1"
                     /translation="MRVVLDSNILFSALISPHSYPHTIYCAWRAARFELVTSRHQLDE
                     IRRASRYPR"
     gene            complement(158..397)
                     /locus_tag="VFX11_14170"
     CDS             complement(158..397)
                     /locus_tag="VFX11_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX4939819.1"
                     /translation="MDTVRWNLAVSADTDQALRLFLASQGGGRKGDLSRFVEEAVRAH
                     ILELTAEQAKAANAEVNEDDLSAMVDEALNWARQR"
     gene            complement(431..1384)
                     /locus_tag="VFX11_14175"
     CDS             complement(431..1384)
                     /locus_tag="VFX11_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX4939820.1"
                     /translation="MPTRAQYIEAATRDNTRRSYRAALRHYEEEWGGLLPATADSVSR
                     YLTDHADTLSVNTLKQRLAALAKWHNDQGFPDPTKAPLVKTILKGIRRLHPAVEKQAK
                     PLQLEQLEIVDTWLRHQADNSNRDAAMQLRCIRDRALLLLGFWRGFRSDELCRLRVEH
                     VTLTPGEGLTLFLPTSKGDRDNQGRTFRAPALRALCPVEAVNTWLTLTGRTEGPLFPR
                     IDRWGRLSETPMNPGSINKWLRELFTKAGLPESERYSSHSLRRGFAGWANANQWDVKT
                     LMQYVGWSDVQSALRYIDGADPFAAFRSPRTALASPPIIDV"
     gene            1597..2559
                     /locus_tag="VFX11_14180"
     CDS             1597..2559
                     /locus_tag="VFX11_14180"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX4939821.1"
                     /translation="MNKFNVQQARDTLLARGINPSVDAVRVELGNTGSKTTIHRYLKE
                     VEAEDATQLSDEALLSNALKDMVARMAAKLRDEALQRAEAANQAWQTEKTQLIAQLSA
                     TQARLKELSADNTALTTRLERESAAHDETKTRLHNADLRAERFEQQVKGLEQQLDENQ
                     KHLASLEEKHRNSRDALEHFRTSAQEQRQQEQLRHDTQVQQLQAEIRNLNQTLIVKQS
                     ELTQLNRDNASLVGEVGEHRKQAKQLEKSLSEKTKQADLASDTNTKLLAVTEEKNALD
                     SRLQSLSSTLETELEKNMAMVLSLAKLQTELDVKNALLEKLSIR"
BASE COUNT          639 a          771 c          775 g          518 t
ORIGIN      
        1 cgcggataac gactggctcg gcgaatctcg tccagttggt ggcgcgacgt caccagctcg
       61 aaacgggctg cccgccaggc gcagtaaatg gtgtgggggt agctgtgggg tgagatcagg
      121 gcactgaaaa gaatgttgct gtccagaacg acccgcatca gcgttgccgc gcccaattca
      181 aggcttcatc caccatggcg ctcaagtcat cttcattgac ctcggcattg gcggccttgg
      241 cctgttccgc ggtcaattcc aggatatgcg cacgcaccgc ttcctctacg aagcgcgaaa
      301 gatcgccctt gcgcccgccg ccctgactgg ccaggaacag ccgcagcgct tggtcggtgt
      361 cggcggacac ggccagattc caacgtacgg tatccatagc atttgctcca cttaataggt
      421 gtttatgtgt ttatacatct attataggcg gtgacgcaag ggccgttctg ggggaacgga
      481 aggccgcaaa cggatcggca ccgtcgatat aacgcagggc cgactgcaca tcactccatc
      541 ccacgtattg catcagggtc tttacatccc attggttggc attggcccac ccggcaaacc
      601 cgcgtcggag cgaatggctg ctgtaccgct cggactcagg cagaccagcc ttggtaaaca
      661 actcgcgcag ccacttgttg atactgccgg gattcatggg cgtctcggac agacgccccc
      721 agcgatcgat gcggggaaac aatggcccct ccgttctgcc ggtcagagtc agccaggtat
      781 tgacggcctc caccgggcac agtgctcgta aggctggtgc ccgaaaggtg cgcccctgat
      841 tgtcccgatc gcccttgctg gtggggagaa agagcgtcaa tccctcgccc ggagtgagag
      901 tcacgtgttc cacccgcagg cgacatagct catcgctgcg aaagccccgc cagaaaccca
      961 gcaacagcag cgcccgatct cggatacacc gcaactgcat cgccgcatct ctattggagt
     1021 tatccgcctg gtgacgcagc cacgtatcga cgatctccag ttgctccagt tgcaagggtt
     1081 tggcctgttt ctcaacggcg ggatgcaggc gccgtatgcc cttgagtatg gtttttacca
     1141 gcggtgcttt ggtgggatca ggaaagcctt gatcgttgtg ccatttggcc agcgccgcca
     1201 gccgctgctt gagtgtattg actgacagcg tgtcggcgtg atcggtcagg tagcggctga
     1261 cgctgtcggc ggttgcaggc aacagtccgc cccattcttc ttcgtaatgc cgaagtgcgg
     1321 cccgatagct gcgacgggtg ttatcccgcg tggcagcctc gatgtactgg gcacgggtcg
     1381 gcatgggggc ctcctgatca ggcgctggaa aaccgggatt cgtaacggtt gagcccgagt
     1441 ttaccgcgaa aaaccagcct ccgataggat aacactttat tacccgatct atagtttatt
     1501 cgaatgctgt acacgttacc tcaacactat agaatgtatt tacatagtat gtaatatatt
     1561 atcttaaaat gggaggccgg aatggcgcgg gcgggcttga acaagttcaa tgtgcagcag
     1621 gcgcgggaca cgctgttggc gcggggtata aacccgtcag tggacgcggt acgggttgaa
     1681 ctgggcaata cgggctcgaa gacaacgatc caccgctatc tgaaggaggt ggaagccgag
     1741 gatgccaccc aactgagtga cgaagcctta ctgtccaatg ccctgaagga catggttgcc
     1801 cgcatggccg ccaagctgcg tgacgaagcc ctgcaacggg cggaagccgc caatcaagcc
     1861 tggcagaccg aaaagacgca gctgattgcg cagctcagtg ccacgcaggc cagactcaaa
     1921 gagttgagcg ccgacaacac tgccctcacg acccgtctgg agcgcgagag cgcagcgcac
     1981 gatgaaacca aaacccgcct ccataatgcg gatctgcggg ccgagcgctt cgagcagcag
     2041 gtgaaagggc tggaacaaca gctcgacgaa aaccagaagc acctggcatc attggaagaa
     2101 aagcatcgga attcgcggga cgcattggaa cacttccgca cctccgccca ggagcaacgt
     2161 caacaggagc aactgcgcca cgacacccag gtccagcagt tacaggctga aatacgcaac
     2221 cttaatcaga ccctgatcgt caaacagagc gaactcactc agctcaatcg tgacaatgcc
     2281 agcttggtcg gtgaagtggg tgagcaccgg aaacaggcga aacaactgga aaagagcctc
     2341 tctgagaaaa cgaagcaagc ggatctcgcc agtgacacca acacaaaact ccttgctgtg
     2401 accgaagaga agaacgcgct tgattctcga cttcagtcat tgtcgagcac tcttgaaact
     2461 gaactcgaaa aaaacatggc gatggtgctg agcctggcca aactacaaac agaactggac
     2521 gtgaaaaacg cactgcttga gaagctttcc attcgatgaa gcatcgatgc cggtcggcag
     2581 caaagggcga agagcggaag tttaatcgat caatcttaac gtccgctgtt tccaagccca
     2641 acaccaggac tcagggcgct ggtaatgcgg acgcttggaa ttctacgcga ttgcggcccg
     2701 ctt
//
LOCUS       DASUEG010000749         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_137944, whole genome shotgun sequence.
ACCESSION   DASUEG010000749 DASUEG010000000
VERSION     DASUEG010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137944"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..545)
                     /locus_tag="VFX11_14185"
     CDS             complement(69..545)
                     /locus_tag="VFX11_14185"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX4939822.1"
                     /translation="MLNLKKVLPVLSLWMFAAVAWAGNAIEVKDGWVRWVPPVSENSA
                     AYFTLHNPTDKPLELKSASSDVARAVELHNVVKDGDLMSMQKQEKIGIPAHQTLQFQP
                     GSYHVMLIGLKKPLEENQSVSLTLHFADGTAVPVSLTVRNDAGSTPAADEHHHHHH"
     gene            complement(628..1212)
                     /locus_tag="VFX11_14190"
     CDS             complement(628..1212)
                     /locus_tag="VFX11_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4939823.1"
                     /translation="MIARWLACGGMVALLGWPLLLAAKELPVNTRLGGDFQLQSTVAA
                     QDQLSDFKGRLVLLNFGYTSCPDVCPMVLSRMAQVMNALEEDSQETRARVQPLFVTFD
                     PARDTVERLQAYLQHFGKDFIGMTGTPEQVADVAKRFGVVYLPQKSDSAAGTLFAHSD
                     YIYLLDDQGRVRALFGSKDPIDEMVDAVESLLDD"
     gene            1351..>2064
                     /locus_tag="VFX11_14195"
     CDS             1351..>2064
                     /locus_tag="VFX11_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018916710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4939824.1"
                     /translation="MRKKLFIETHGCQMNEYDSSRMADLLGVTHQLELTDNAEEADVL
                     LLNTCSVREKAQEKVFHQLGRWKKLKEKKPDLLIGVGGCVASQEGDAIRQRAPFVDLV
                     FGPQTLHRLPKMIEVVDLSHEAVVDVSFPEIEKFDTLPQPSVDGPKAYVSIMEGCSKY
                     CTFCVVPYTRGEEVSRRFEDVMQELQHLAGLGVREVTLLGQNVNGYRGENASGEIVDF
                     AELIEHTARIPGIDRIRYTT"
BASE COUNT          445 a          657 c          563 g          399 t
ORIGIN      
        1 tggcgaaagc cttccgcaga aaagtcgaat tcgtcaaaac ctgccaggga atgcgtcatg
       61 gcggcacatc aatgatgatg gtggtggtgt tcgtcggccg ctggcgtgct gccggcatca
      121 ttgcgcaccg tcagcgacac cggaaccgcg gtgccatcgg caaagtgcaa ggtcagcgac
      181 accgactggt tttcctccag cggcttcttc aacccgatca gcatcacatg ataggaaccg
      241 ggctggaatt gcagggtctg gtgcgcggga atcccgattt tttcctgctt ttgcatcgac
      301 atcagatcgc cgtccttgac cacgttgtgc aactccaccg cacgggcgac atcgctgctg
      361 gcggatttca gttccagcgg tttgtcagtc gggttgtgca gcgtgaaata ggccgcactg
      421 ttctcactga ccggcggcac ccagcgcacc cagccgtcct tcacttcaat ggcattgccc
      481 gcccaggcga cagcggcgaa catccacaac gacaaaaccg gcaatacttt tttcaggttc
      541 aacatggtta acatctccag caaaacatcg cgattctttc tgttcatctg cttcgaccgg
      601 caagggtatt cacggcttca ccatttttca gtcatccagc agactttcaa ccgcatccac
      661 catctcgtcg atggggtcct tgctgccgaa cagtgcacgc acgcggccct ggtcatccag
      721 cagatagata tagtcactgt gggcgaacag ggtgccggcg gcggaatcgg atttctgcgg
      781 cagatacacc acgccaaagc gtttcgccac atcggccacc tgctctggcg tgccggtcat
      841 gccgataaaa tccttgccga aatgctgcag gtacgcctgc aaccgctcca ccgtgtcacg
      901 cgcgggatcg aaggtcacga acagcggctg tacccgcgcc cgcgtttcct gcgaatcctc
      961 ctccagcgca ttcatcacct gcgccatacg cgacagcacc atggggcaca catcggggca
     1021 ggaggtataa ccgaaattca gcagcaccag ccggcccttg aaatcgctca actgatcctg
     1081 cgccgccacg gtggactgca gttgaaagtc cccacccagc ctggtgttaa ccggcaattc
     1141 cttcgccgcc agcagcagcg gccatcccag caacgccacc attccaccac aagccagcca
     1201 tcgtgcaatc acgctttcct cctgattccc cagcggtttt tcaagtcacc ggatagtaac
     1261 ccattgccgc tggtgtacaa actgtgaaaa ccaggggtgc acctgtataa tgcgcctccg
     1321 atcctgtcca cccgatgttc tgaattcccc atgcgcaaga aactctttat cgaaacccac
     1381 ggctgccaga tgaacgaata cgattcgtcc cgcatggcgg acctgctggg cgtcacccac
     1441 cagctggaac tcaccgacaa cgccgaggaa gccgacgtgc tgttgctgaa cacttgttcg
     1501 gtgcgggaaa aggcgcagga gaaagtattc caccagctgg ggcgctggaa gaagctgaaa
     1561 gagaaaaaac cggacctgct gatcggcgtc ggcggttgcg tggccagcca ggaaggcgat
     1621 gccatccgtc agcgcgcgcc gttcgtggat ctggtgttcg gcccgcaaac cctgcaccgg
     1681 ctgccgaaaa tgatcgaggt ggtggacctg tcgcacgaag ccgtggtgga cgtctccttc
     1741 cccgaaatcg aaaaattcga caccctgccg cagcccagcg tcgatggccc caaggcctac
     1801 gtctccatca tggaaggttg cagcaaatac tgcacgttct gcgtggtgcc ctacacccgg
     1861 ggcgaggaag tcagccgccg ctttgaagac gtgatgcagg aactgcagca tctggccggt
     1921 ctgggcgtgc gcgaagtgac cctgctgggc cagaacgtga acggctatcg cggcgagaac
     1981 gccagcggcg agatcgtgga ttttgccgag ctgatcgagc ataccgcccg cattcccggc
     2041 atcgaccgca tccgctacac cacc
//
LOCUS       DASUEG010000750         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_10058, whole genome shotgun sequence.
ACCESSION   DASUEG010000750 DASUEG010000000
VERSION     DASUEG010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10058"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFX11_14200"
     CDS             <1..295
                     /locus_tag="VFX11_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939825.1"
                     /translation="CQQLAASDTLHFARILALLMQNHGPHGYYRWLPQQSAAVINTQT
                     EGSPYATRGDLLRQAVLETLRALLRLSPKRELGWLRFRSGLFQKIYTRLYGHH"
     gene            307..2199
                     /gene="asnB"
                     /locus_tag="VFX11_14205"
     CDS             307..2199
                     /gene="asnB"
                     /locus_tag="VFX11_14205"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4939826.1"
                     /translation="MCGIAGFVRFQHPTGDIDTLKAMGNAIFHRGPDAGGEYIDDGVG
                     LCHRRLAIIDLSPAGVQPMHSVNDRYVIVFNGEIYNFQELREDLIAKGYPFRSRSDTE
                     VLLALYQHEGEALVEKINGMFAFALWDKQEQVLFIARDRLGKKPLYFYQHEGYFIFGS
                     ELKAILPLPMVKRDLRPDAIYDFFTYQYVPDPKTIFTHIHKLEPAHTLTITRNGIRKR
                     QYWDVSFKNQQQRDADAIADDLLGIINHCVKSRMVSDVPLGAFLSGGVDSSGVVGLMA
                     QNSDKPVTTCSIGFDSEKFDEVKYAAEIARQFKTDHHEFTVKENVENNLEKIAAFFDE
                     PFADPSLVPTYFVSQLARQQVTVALAGDGGDESFAGYSKYAVDAVENRLRNLFPAFLR
                     HSLFPGLAKLFSKSHATVARKATTLLTTLSHDPGYGFFMSNSFFSDELWRRVVRDDFS
                     RTLGSYHPSSLSLQHYNNAPADDHLSRILYTDIKTYLPGDILVKADRMSMANSLEVRA
                     PLLDYVLVEYAASIPSALKLNGNDKKFILKKAFSKLLSHDTLYRKKMGFSVPLAEWLR
                     QTLRTKAEKLLFTSQHGLPDYFKMDQVQAIWNDHQQGKRDYSSELWSMLMFQLWYDRY
                     VTGATA"
BASE COUNT          492 a          701 c          578 g          441 t
ORIGIN      
        1 atgccagcaa ctcgcggcca gcgacacgct gcattttgcc cgcatactgg cgctgctgat
       61 gcagaaccac gggccacacg gttattaccg ctggctgccg cagcaatccg ccgctgtcat
      121 caatacgcaa accgaaggca gcccttacgc cacccgcggc gacctgctgc gacaagcggt
      181 gctggaaacc ctgcgcgcgc tgctgcgcct gtcaccgaaa agggaactgg gctggctgcg
      241 attccgcagc ggactgttcc agaagatcta cactcgtctt tacggccatc attaacggaa
      301 tcccttatgt gcggcattgc aggctttgtt cgcttccaac acccgaccgg tgacatcgac
      361 accctgaagg ccatggggaa cgccattttc caccgcggcc ccgatgccgg cggcgaatac
      421 atcgatgacg gtgtgggtct gtgccaccgc cgcctcgcca tcatcgacct gtctcccgcc
      481 ggtgtgcagc cgatgcactc cgtcaacgac cgctatgtca tcgtcttcaa cggcgagatc
      541 tacaacttcc aggaactgcg cgaggatctg atcgcgaagg gctacccgtt ccgctcccgg
      601 tccgacaccg aagtgctgct ggcactgtac cagcatgaag gcgaagccct ggtggagaag
      661 atcaacggca tgtttgcgtt tgccctgtgg gacaagcagg agcaggtgct gtttatcgcc
      721 cgcgaccgcc tcggcaaaaa gccactgtat ttctaccagc acgaaggcta tttcattttc
      781 ggctctgaac tgaaagcgat cctgccactg ccgatggtga aacgcgacct gcgccccgat
      841 gccatctacg acttcttcac ctaccagtac gtgcccgatc ccaaaaccat ttttactcat
      901 atccacaaac tggaaccggc ccatacgctg accattaccc gcaacggaat ccgcaagcgg
      961 cagtactggg atgtgtcatt caaaaatcag cagcagcgcg atgccgacgc catcgccgac
     1021 gacctgctgg gcatcatcaa ccactgcgtg aaaagccgca tggtgagcga tgtgccgctg
     1081 ggcgcctttc tcagcggcgg cgtcgattcc agtggggtgg tgggtctgat ggcgcagaac
     1141 agcgacaagc cggtgaccac ctgttccatc ggtttcgatt cagaaaaatt cgacgaagtg
     1201 aaatacgcgg cagaaatcgc ccgccagttc aagaccgacc accacgaatt caccgtcaag
     1261 gaaaacgtcg aaaataatct ggagaaaatc gccgcgtttt ttgatgagcc ctttgccgat
     1321 ccgtcgctgg tgccgacgta ctttgtgtcg caactggcgc ggcagcaggt taccgtggcc
     1381 ctggccggcg acggcggcga cgaaagcttt gccggttaca gcaaatatgc ggtggatgcc
     1441 gtggaaaatc gcctgcgcaa tctgttcccg gccttcctgc ggcactcact gtttcccgga
     1501 ctggcaaaac tgttttccaa aagccatgcc accgtcgccc gcaaggccac cacgctgctc
     1561 accaccctga gccacgatcc gggctacggg tttttcatgt ccaattcgtt cttcagcgac
     1621 gagctctggc gccgtgtcgt gcgcgatgat ttcagccgca ctctcggttc ttaccatccc
     1681 tccagtctct cgctgcagca ctacaacaac gcgccggcgg atgaccacct gtcccgcatt
     1741 ctctataccg acatcaaaac ctacctgccc ggcgacattc tggtaaaggc agaccgcatg
     1801 agcatggcca attcgctgga agtgcgcgca ccgttgctgg attacgtatt ggtggaatac
     1861 gctgcctcga tcccctcagc actgaaactg aacggcaacg acaagaaatt catcctgaaa
     1921 aaagccttca gcaaactgct cagccacgac accctgtatc gcaagaaaat gggcttctcg
     1981 gtgccgctgg cagaatggtt gcgtcaaacc ttgcggacaa aagcggaaaa gctgctgttc
     2041 acatcgcagc acggcctgcc ggattatttc aaaatggatc aggttcaggc catctggaac
     2101 gatcaccagc aaggcaaacg cgattactcc agtgaactct ggagcatgct gatgttccag
     2161 ctgtggtacg accgctacgt caccggcgcg acagcctgaa ccgttcaccc ct
//
LOCUS       DASUEG010000751         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1025151, whole genome shotgun sequence.
ACCESSION   DASUEG010000751 DASUEG010000000
VERSION     DASUEG010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025151"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFX11_14210"
     CDS             <1..503
                     /locus_tag="VFX11_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4939827.1"
                     /translation="KDSIIKPGMTVLDLGAAPGGWSQVAAQLVGDKGLVIASDILEMD
                     NIAGVQFLQGDFREETVYRQLLEIIGDRPVDLVISDMAPNMSGVDAVDQPRAMYLAEL
                     ALEMAGQVLKKDGAFLVKVFQGGGLPEYRKELQQKFATLTTRKPEASRARSKETYLLA
                     KGFRGA"
     gene            628..>2001
                     /gene="ftsH"
                     /locus_tag="VFX11_14215"
     CDS             628..>2001
                     /gene="ftsH"
                     /locus_tag="VFX11_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX4939828.1"
                     /translation="MAKNLILWVVIAIVLLSVFNNFSSQGSTQRIPYSKFVEQVEGGQ
                     VDQVVIDGSIVTGKTVANEKFETVLPNLFDVDLMPMLLKNNVAVEGRQPEKQSLWAQL
                     LVACFPILLIVAIFMFFMRQMQGGGGGRGGPMTFGKSRARLLSEDQIKTTFADVAGCD
                     EAKDEVQELVEFLRDPGKFQRLGGKIPRGVLMVGPPGTGKTLLAKAIAGEAKVPFFTI
                     SGSDFVEMFVGVGASRVRDMFEQAKKQAPCIVFIDEIDAVGRSRGAGLGGGHDEREQT
                     LNQLLVEMDGFEPNDGVIVIAATNRPDVLDSALLRPGRFDRQVVVSAPDIRGREQILK
                     VHMRKVPLGDDVEPKTIARGTPGFSGADLANLVNEAALFAARSGKRVVGMAEFEKAKD
                     KILMGTERKTMVMSEKEKRNTSYHEAGHAIVGRLVPEHDPVYKVSVIPRGRALGVTMY
                     LPEEDKYS"
BASE COUNT          407 a          556 c          620 g          418 t
ORIGIN      
        1 aaaaagacag catcatcaaa cccggcatga ccgttttgga tcttggcgcc gcgcccggtg
       61 gctggtccca ggtggcggcg cagctggtgg gcgacaaggg tctggtgatc gcctccgaca
      121 tcctggaaat ggacaacatt gccggcgtgc aattcctgca gggcgacttc cgcgaagaga
      181 ccgtctacag acaactgctt gaaatcattg gcgaccgccc ggtggacctt gtaatttccg
      241 acatggcccc caatatgagt ggagtggatg ccgtcgatca gccccgtgcc atgtacctgg
      301 cggagctggc gctcgaaatg gccggtcagg tgctgaagaa ggatggtgcc tttctggtca
      361 aggtgttcca gggcggcggt ctgccggaat accgcaagga attgcagcag aaattcgcca
      421 ccctgaccac ccgcaagccc gaagcctccc gcgcccgttc caaggaaacc tacctgttgg
      481 ccaagggttt tcgcggggcg tgaagcgggt ccggcgaaag atggtgccag cgtctattgt
      541 gtagtaatgt agcaaaaaca tggtttcaga acgcagtcgc gccgcgtctg ccggttgggc
      601 gagagagagg gtaacccctt gaacgatatg gcaaagaatt tgattctctg ggtcgtgata
      661 gcaattgttt tgctgtcggt tttcaacaac ttcagttccc agggcagcac ccagcgcatt
      721 ccctattcca agttcgtgga acaggttgaa ggcggtcagg tggatcaggt cgttatcgac
      781 gggtccatcg tcaccggcaa gaccgtggcc aacgagaaat tcgaaaccgt tctccccaat
      841 ctgtttgatg tcgacctgat gccgatgttg ctgaaaaaca acgtcgctgt cgaaggtcgc
      901 cagccggaaa aacaaagcct gtgggcccag ctgctcgtcg cctgtttccc gattctgctg
      961 attgttgcga tcttcatgtt cttcatgcgc cagatgcagg gtggcggtgg tggtcgtggc
     1021 ggtcccatga ccttcggcaa gagccgtgcc cgcctgctca gtgaagacca gatcaaaaca
     1081 acctttgccg atgtggccgg ctgtgacgaa gccaaggacg aagtgcagga gctggttgag
     1141 ttcctgcgtg acccgggcaa attccagcgt ctgggcggca agattccgcg cggcgtattg
     1201 atggtgggcc ctcctggtac cggtaaaacc ctgttggcga aagccatcgc cggcgaagcc
     1261 aaggtgccgt tcttcaccat ctccggttct gatttcgtgg aaatgttcgt gggtgtgggt
     1321 gcatcccgcg tacgcgacat gtttgaacaa gccaagaagc aggcgccctg tatcgtgttc
     1381 atcgacgaga tcgatgccgt gggccggtcc cgtggcgccg gtctgggcgg tggtcacgac
     1441 gagcgcgagc agacgctgaa ccagctgctg gtggaaatgg acggctttga gcccaatgat
     1501 ggtgtcatcg tcattgctgc caccaaccgt cctgacgtac tggattccgc attgctgcgt
     1561 cccggccgtt tcgatcgtca ggtggtggtg tccgcccccg atatccgggg tcgcgagcag
     1621 atcctcaagg tgcacatgcg caaggttccc ctgggtgacg atgtcgagcc caaaaccatt
     1681 gctcgcggca cgcccggttt ctccggcgct gatctggcca acctggtcaa tgaagccgcg
     1741 ttgtttgcgg cgcgttccgg caagcgcgtg gtgggcatgg ccgagttcga gaaagccaag
     1801 gacaagatcc tgatgggcac cgaacgcaag accatggtca tgtcggaaaa agagaagcgc
     1861 aatacgtcct atcatgaagc cggccacgcc atcgtcgggc gtctggtgcc tgagcatgat
     1921 ccggtttaca aagtcagtgt cattccccgc ggtcgtgcgc tcggcgtcac catgtacctg
     1981 ccggaagagg acaagtacag c
//
LOCUS       DASUEG010000752         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_517401, whole genome shotgun sequence.
ACCESSION   DASUEG010000752 DASUEG010000000
VERSION     DASUEG010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517401"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..914)
                     /locus_tag="VFX11_14220"
     CDS             complement(12..914)
                     /locus_tag="VFX11_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4939829.1"
                     /translation="MNYRHILLALLCAFLWGANFAVIKWGLQDIPALAFASLRFGAVA
                     LLAFFCARPTVPVTALMRYGIGWGTLQFGGLFLAIHAGMPTGLASVLAQSQAIFTLLF
                     AALAGHDALRPFHYLAILTAFTGITAIAVDQTAPIPPLALLLSLSGAAGWAYGNLAIR
                     RFSLAGHRVDTIGFVAWASIIPALTLALLSTIFEDPARLAQLEPAGALRAAMAVLYQA
                     GAALLFGTLVWNSLLRHYPAPSVAPFSLVVPVVGLSLGVLLLNETISVLQGIGYSLLL
                     AALAINLLGLRSRLHAQESGERKR"
     gene            complement(1160..2011)
                     /gene="panC"
                     /locus_tag="VFX11_14225"
     CDS             complement(1160..2011)
                     /gene="panC"
                     /locus_tag="VFX11_14225"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX4939830.1"
                     /translation="MQTVNSIQELRAIIKAQRIAGKRIGFVPTMGNLHEGHITLVREG
                     QKRADFMVSSVFVNPTQFGAGEDFDKYPRTLKEDQEKLSAAGCNLLFAPPVDEMYPGD
                     QASWATVHVTKVGERHCGASRPGHFDGVSTVVTKLFNIVQPDVALFGKKDFQQLAVIR
                     RMTTALCFGIEIIGVDTVREASGLAMSSRNGYLSAEEKQKAATIYQTLCRTREAIRSG
                     EKNFAALEQTANATLAQAGFQPEYFTVCRADTLEPAGASDKNLVILVAAKMGKTRLID
                     NIDFVTA"
     gene            complement(2063..2857)
                     /gene="panB"
                     /locus_tag="VFX11_14230"
     CDS             complement(2063..2857)
                     /gene="panB"
                     /locus_tag="VFX11_14230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX4939831.1"
                     /translation="MAITLSTLNKLKQQGQKFAVLTAYDSTFANLASTAGVEVLLVGD
                     SLGNVLQGHDSTVPVTLQDMIYHTECVRRGNQGALIMSDLPFMTYATEAQTFESCAAL
                     MRAGAHVVKLEGGAWLTESVRKLTDRGVPVCIHLGLTPQSVNKFGGYKVQGRDPAERQ
                     ALIADSIAAEKAGAAILLYECIPSDLMQEIMTKVSIPVVGIGAGNSTDAQVLVLHDML
                     GATTRKPAKFVKNFMSAGDGSIQGAIAAYVKAVKDGSFPAAEHSFD"
     gene            complement(2926..3591)
                     /locus_tag="VFX11_14235"
     CDS             complement(2926..3591)
                     /locus_tag="VFX11_14235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX4939832.1"
                     /translation="MLERQQGQHPPLPRFIVVEGPIGVGKTTLTHKLAQTFGYDTLLE
                     GADENPFLDRFYKNSKQWALQTQLYFLFQRRDQLNALRQNDLFEPVRVADFLIQKDKL
                     FASITLDDDEFALYEKIYQHLIQATPKPDLVIYLQAPVDILQQRIAKRGIGSEQGITD
                     AYLHRLSEAYNRFFLYYDEAPLLMVNTAEINLAESARDYENLVQYILTIRSGRHFFNP
                     AAG"
     gene            complement(3601..4104)
                     /gene="folK"
                     /locus_tag="VFX11_14240"
     CDS             complement(3601..4104)
                     /gene="folK"
                     /locus_tag="VFX11_14240"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005329042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4939833.1"
                     /translation="MISAFLGLGSNLQSPRAQLQRCIDALTQLPGTELIAVSPFYGSA
                     AVGPGTQPDYVNAVAQIETALAPQDLLTALQQIEQEQGRERGPERWLPRTLDLDILLY
                     GDQVVNLPHLHIPHPRMHERSFVLLPLFDLAPDLALPDGQPVRQLLQRCSRDGIWLLE
                     DTHTGDR"
     gene            complement(4146..5513)
                     /gene="pcnB"
                     /locus_tag="VFX11_14245"
     CDS             complement(4146..5513)
                     /gene="pcnB"
                     /locus_tag="VFX11_14245"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX4939834.1"
                     /translation="MPKRLLQKITGLIKGKKSSPVQRREIPRGEHHFPEEDIGRNVIS
                     VLDTLTRAGYEAYLVGGSIRDGLIGMHPKDFDVATSATPEEIRAQFRNCRLIGRRFRL
                     AHVRFGRDIIEVATFRAAHHTMTEEDVDQSHQSDEGMLLRDNVFGTLDEDAVRRDFTA
                     NALYYRHTDGAVLDFVNGYEDIRHKTLRLIGNPEQRYREDPVRMLRAVRFAAKLGFTI
                     EPATAEPIKRLSDLLGLIPPARLFEEIQKLFLSGHAEAVWPLLTRYDLARWLFPLSVP
                     ENNDAPGAQAMIALALRNTDLRIHNGKGVTPYFLLAVLLWGPLQKLWREYCEDGIPPT
                     PALQKAIQQVIHDQSRHVAIPKRFSLPMREIWELQSRLDQRSAKRCEQLVQQPRFRAA
                     YDLLLLREESGETEAGLGEWWTAYQQADAAEREQMCQQLGQKPARKRRRYNNRRKGAR
                     PAPPQ"
BASE COUNT         1070 a         1613 c         1779 g         1158 t
ORIGIN      
        1 gaaccagagg gtcatctttt tcgctctcca gactcttggg cgtggagacg tgaccgcagt
       61 cccagcaggt tgatggccag cgctgccagc agcagcgagt agccaatgcc ctgcaacacg
      121 gagatggttt cattcagcag cagcacgccc agcgacagtc cgaccaccgg cactaccagc
      181 gagaacggtg ccaccgaggg cgcgggatag tgccgcagca aactgttcca gaccagcgtg
      241 ccgaacagca gggccgcgcc tgcctggtac agcacggcca ttgccgccct cagcgcgccg
      301 gccggttcaa gctgggcaag gcgcgcaggg tcttcgaata tggtactcag caatgccagc
      361 gtcagcgcag gaatgatgga ggcccaggcc acgaagccga tggtatccac ccggtgcccc
      421 gccagtgaga agcggcggat ggccagattg ccgtaggccc agccggcggc gccggacagg
      481 ctcagcagca gtgccagtgg cggaatcggc gcagtctggt ctaccgcaat ggcggtgatg
      541 cccgtgaatg cggtgaggat ggccaggtaa tgaaaggggc gcaacgcatc gtgcccggcc
      601 agcgcggcaa acagcagggt gaagatggcc tgtgactggg ccagtaccga tgcgagcccg
      661 gtgggcatgc cggcatggat ggcgagaaac agtccgccaa actgcagcgt tccccagccg
      721 atgccatacc gcatcagcgc ggtgactggc acggtggggc gggcgcagaa aaatgccagc
      781 agagccaccg cgccgaagcg gagactggca aaagccagcg ctggaatatc ctgcaggccc
      841 catttgatga cggcgaaatt ggcaccccac agaaaagcgc agagcagggc tagcaggatg
      901 tgccggtagt tcatggtatt cctgtaaaga aaggcaatga tcaggacctg cgttctgccg
      961 gcggaaaccg cacacggacg gcaaccgcct ggcgcctgta aacgtatgcc atcgtggcgg
     1021 ccgttagaaa tgaaaaaatg cgggtgtcac atgcaggata ttcatgaacc tcagcggccg
     1081 gtacaaacac aggccgctga cgaataatcg cttttgttct ggtgggcagt aaccggtagc
     1141 gatgcccggg ctgaaaggat caggctgtca caaaatcgat attgtcgatc agtcgcgtct
     1201 tgcccatctt ggccgctacc agaatcacca gattcttgtc gcttgcgccg gcgggttcca
     1261 gcgtgtcggc gcggcaaacc gtaaaatatt ccggctgaaa tccggcttgc gccagcgtgg
     1321 cattggcagt ctgctccagc gcggcaaaat ttttctcgcc ggagcggatc gcttcccgcg
     1381 tgcggcacaa tgtctggtaa atggtggcgg ccttctgttt ttcttccgca ctcagatagc
     1441 cgttgcgcga actcatggcc aggccgctgg cctcgcgcac cgtgtccacg ccgatgatct
     1501 cgatgccgaa gcacagtgcc gtggtcatgc gacggatcac cgccagttgc tggaaatcct
     1561 tcttgccgaa caaggccacg tcaggctgca cgatattgaa cagcttggtg acgacagtgg
     1621 acacgccatc gaaatggccg gggcgggaag cgccacagtg acgttcgccc accttggtca
     1681 catgcacggt ggcccagctg gcctgatcgc ccgggtacat ctcgtccacc ggtggcgcga
     1741 acaataaatt gcagccggca gcgctgagtt tctcctggtc ttctttcagc gtgcgcggat
     1801 acttgtcgaa gtcttcccct gcgccgaact gcgtcggatt cacaaacacg ctcgacacca
     1861 tgaagtccgc tcttttctgg ccttcgcgca ccagcgtgat atggccttcg tgcaggttgc
     1921 ccatggtggg tacgaaacca atgcgtttgc cggcgatacg ctgggctttg atgatggcgc
     1981 gtaattcctg gatgctgttg accgtctgca taagtgtcct atgaaaggca attttgtgag
     2041 cgaaagtcct gtgaacggaa aattagtcga atgaatgctc ggcagcaggg aagctgccgt
     2101 ctttcactgc tttcacatag gcggcaatgg cgccctggat actgccgtcg ccggcgctca
     2161 tgaaattctt cacaaacttg gcgggcttgc gcgtggtggc gcccagcatg tcgtgcagca
     2221 ccagtacctg cgcgtcagtg gaattgccgg cgccgatgcc gaccaccgga atactcacct
     2281 tggtcatgat ttcctgcatc agatcagacg gaatgcattc gtacagcaga atggctgcac
     2341 cggctttttc tgcggcgatg gaatcggcga tcagtgcctg ccgttcggcc ggatcgcggc
     2401 cctgcacttt gtagccaccg aatttgttca ccgattgcgg cgtcaggccc aggtgaatgc
     2461 acaccggcac gccgcggtca gtcagtttgc gcacgctttc cgtcagccag gcgccacctt
     2521 ccagtttcac cacatgcgca ccggcacgca tcagcgcggc gcagctttca aaagtttgtg
     2581 cttctgtcgc ataggtcatg aacggcagat cggacatgat cagcgcgccc tggttgccac
     2641 gacgcacaca ctcggtgtga tagatcatgt cctgcaacgt gacgggcact gtgctgtcat
     2701 ggccttgcag aacgttgccc agcgaatccc ccaccagcaa cacttccacg ccggccgtgc
     2761 tggccaggtt cgcgaacgtg ctgtcgtacg cggtgagcac ggcgaatttc tggccctgct
     2821 gcttcagctt gttgagtgtg gagagagtga tggccatggt gaagtgtccc tgttgtggcg
     2881 tgcgatgtgg cgcgcgtgat tgcgccggag ctggtggctc cggaatcagc ccgcagcggg
     2941 attgaagaaa tggcggccgc tgcgaatcgt cagaatatac tgcaccagat tttcgtagtc
     3001 acgggcgctt tctgccaagt tgatttcggc agtgttcacc atcagcagcg gcgcttcgtc
     3061 gtaatacagg aagaaacggt tgtaggcttc gctgagccgg tgcagatagg catcggtaat
     3121 gccctgttcg ctgccgatgc cacgtttggc gatgcgttgc tgcaggatat ccacgggcgc
     3181 ctgcaggtaa atcaccagat ccggcttcgg tgttgcctgg atgaggtgct ggtaaatttt
     3241 ttcgtacagt gcgaattcgt cgtcatccag cgtgatggag gcgaacagtt tgtctttctg
     3301 gatcagaaaa tccgccaccc gcaccggttc gaacagatcg ttctggcgca gagcgttcag
     3361 ctggtcgcgg cgctggaaca ggaagtacag ctgggtctgc agtgcccact gtttgctgtt
     3421 tttgtagaag cggtcgagga acggattttc atcggcgcct tccagcagcg tgtcgtaacc
     3481 gaacgtctgc gccagtttgt gggtgagggt ggttttgccc acgccgatgg gaccttccac
     3541 cacgatgaag cggggaagtg gaggatgctg accttgctgc cgttctaaca tgggcgcctg
     3601 tcacctatca ccggtgtgcg tgtcttcgag caaccagatg ccgtccctgc tgcaacgttg
     3661 cagcagttgc cgcaccggct ggccatcggg cagcgccaga tccggcgcca gatcgaacag
     3721 cggcagcagc acgaaactgc gctcgtgcat gcgcggatgg ggaatatgca ggtgcggcag
     3781 attcaccacc tgatctccgt acagcaggat gtccagatcc agcgtgcgcg gcagccagcg
     3841 ttccggcccg cgctcgcgtc cctgttcctg ctcgatctgt tgcaacgccg tcagcagatc
     3901 ctgtggcgcc agtgcagttt caatctgcgc caccgcgttc acataatcgg gttgtgtgcc
     3961 gggaccaaca gccgcgctgc cataaaacgg cgacaccgcg attaactctg tgcctggcaa
     4021 ctgcgtcagt gcatcgatgc agcgttgcag ttgtgcgcgg ggggattgca ggttgctgcc
     4081 cagtcccaga aaggcgctga tcatcgcagg tcaaaggctc acggtttgga aattcacgct
     4141 caggcttatt gcggtggagc cggacgagcg cccttgcgcc ggttgttgta gcggcgccgt
     4201 ttgcgggcgg gtttctggcc cagttgctgg cacatctgtt cgcgttcggc tgcgtcggcc
     4261 tgctggtacg cggtccacca ttcgcccagg cccgcttcgg tttcgccgga ttcttcccgc
     4321 aacaacagca aatcgtaggc agcgcgaaaa cgcggctgct gcaccagctg ttcgcaacgt
     4381 ttggcggagc gctggtccag ccggctttgc agttcccaga tttcccgcat cggcaggctg
     4441 aaacgcttgg gaatcgccac gtggcgcgac tggtcgtgaa tcacctgctg aatggctttt
     4501 tgcagggcgg gtgtgggggg aatgccgtct tcgcagtatt cgcgccacag tttttgcagc
     4561 ggcccccaca acagcaccgc cagcaggaaa tagggcgtca cgcccttgcc gttgtgaatg
     4621 cgcaggtcgg tgtttctcag cgccagtgcg atcatcgcct gggcgccagg ggcgtcgttg
     4681 ttttccggta ctgacagcgg gaacagccag cgtgccagat catagcgggt gagcaggggc
     4741 cacacggctt cggcgtggcc gctcagaaac agtttctgga tttcctcgaa cagacgcgcc
     4801 gggggaatca gccccagcaa atccgacagg cgcttgatcg gttcagcggt ggccggttcg
     4861 atggtgaagc ccagtttggc ggcaaagcgc actgcgcgca gcatgcgcac cggatcttcc
     4921 cggtaacgct gttccgggtt gccgatcagg cgcagggttt tgtggcggat gtcttcatag
     4981 ccgttgacga aatccagcac cgcgccgtcg gtgtggcggt aatacagggc gttggcggtg
     5041 aaatcgcgcc gcaccgcgtc ttcatccagt gtgccgaata cgttgtcgcg cagcagcatg
     5101 ccttcatcgg actggtgcga ctggtcgacg tcttcttccg tcatggtgtg atgggcggcg
     5161 cggaaggtgg cgacttcgat gatgtcgcgg ccgaagcgca cgtgggccag gcggaagcgg
     5221 cggccaatca ggcggcagtt gcggaactgg gcgcggattt cctccggggt ggcgctggtg
     5281 gccacgtcga aatccttggg gtgcatgccg atcaggccgt cgcgaatgga gccgcccacc
     5341 aggtaggctt cgtagccggc acgggtcagg gtgtcgagca cgctgatgac gttgcggccg
     5401 atgtcttctt cgggaaagtg gtgttcaccg cgggggattt cgcggcgttg cacagggctg
     5461 gatttttttc ccttgatcag accggtaatt ttctgaagca gccgttttgg catggaagtg
     5521 gcggggttcc tggtgttgtt ctgtggatga aatatggcct gattctatca ctgacactgc
     5581 cagcataata cccaatattc aacaggttag ttgccggtgg
//
LOCUS       DASUEG010000753         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_693676, whole genome shotgun sequence.
ACCESSION   DASUEG010000753 DASUEG010000000
VERSION     DASUEG010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693676"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFX11_14250"
     CDS             complement(<1..322)
                     /locus_tag="VFX11_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939835.1"
                     /translation="MPLRLELKASATGPGYAMLTVIGWKAPAGNVELSVQRNQDSRFL
                     DENGDWTTNPVWHDVSVLEAIEGGLGGEMGPWLVDPLVKNPQVVYMFELRSADHKDKG
                     VLRIL"
     gene            complement(326..3061)
                     /locus_tag="VFX11_14255"
     CDS             complement(326..3061)
                     /locus_tag="VFX11_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative virulence factor"
                     /protein_id="HEX4939836.1"
                     /translation="MSGAEQKTSPEQLSQHWAAVHTGAGDAVRWISDVRRNAPRLDNE
                     ADHLILKMRRVRNLARRLGAVSSRPMTVGFFGLSQAGKSYLISTLAAGANGKLETDFG
                     GQRLDFLTHVNPPGGGKEATGLVTRFSRTAQPGPLDFPIELKLFGEVEVAKILANSFF
                     NDFNTEKFQYEYNDTKVRQMLKDLAKRKQHQPVAGVSEDDVVDLWDYLLDRFPGSLKQ
                     LNGYYWPTAVELAPSLSSDDRAKLFSILWGEIPELTETYARFAQTLASLRHAGSVFAP
                     LDALVRATPQGGLSQADSIMNVDMLERLGKPTDKTISVWPSVNNELQPPVTLSLAQLA
                     ALTAELIFPLVEHTREPLFEDVDLLDFPGYRGRLSVESLDDVRRAVKSDDANPVAQLI
                     LRGKVAYLFERYTDSQEMNVLIVCTPSNKQSDVTSVGPVLTEWIARTQGSTPEIRARR
                     QSGLLWAITMFDMRIGNSLDMGEDLLQIAWGNSGMMKMAMLERFGQYAWMQEWLPGKP
                     FNNTFLVRKPRMKVAFIDLENGEEKAVNAAMAPQLELMKRTFAADETVREHVHEPAAA
                     WDAMMALNDGGMDSVTGYLQKVALRSVKLARIHEQLDDLVHDLVENRLGPWFLAEGAG
                     EMEKKKKIAQQIVDALKPRALLLGDFLQRLQLPDDLLRTLYLRADDEPVATSTPQTGT
                     PQESAGALSLGGDLISLGDDLDLFGSNDSAPKASEKTAQTLAVGSDARFAQAVLREWI
                     NHLRNIPEDQRLMAFFGFPKPAVEALVDELITAANRMDLRTRLLHTIVSTEQVGTKRE
                     QLVDRQVLAARTVLADFIAWLGYIEQPLAERPDSAANKGAKLFEPPAAIAPGSLPDLP
                     PQPINHTGIYLFDWLVAFSKIAVGNAGHSAGREITPEQNDALGRIIATFKATRTE"
     gene            complement(3058..>4224)
                     /locus_tag="VFX11_14260"
     CDS             complement(3058..>4224)
                     /locus_tag="VFX11_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HEX4939837.1"
                     /translation="DILLEVIQKFVVPWFADALKKTGVNDPQPLLSRLIGNEPVKVQE
                     AVLRQQLALQVLSPIGLRLLKEYEKYDPLEPGSLGTFTFADLLAGHEAPTPDVIEFVN
                     AAVRRQLGANATDFNILETPIAVNLRKMHEAFLGNRMNICKTLKALSEIVHCYQCDVL
                     LLTGRPSRLPGIQAVFRSLLPLPPGRILPLHHYRTGGWYPFHKQGRIDDPKTTAAVGA
                     MLCLLGRSLRLPNFFFRAAAFKPYSTIKYLGLIDNNNVVKESNVYYKAINLDDPDYEL
                     PDTPFELRGSMRLGFRQLAAERWSAAPLYTITVVDKKLSEALASEGRVLKVRLQAKRN
                     SANPAEGTERFIIAGVESDTGSVSSRALKLQLNTLSDAGLGESQYWLDSGSVFR"
BASE COUNT          723 a         1300 c         1324 g          877 t
ORIGIN      
        1 ccagaatgcg caacacgccc ttgtccttgt gatcggcgct gcgcagttcg aacatgtaga
       61 ccacctgcgg attcttcacc agcgggtcca ccagccaggg gcccatttcg ccgccgaggc
      121 cgccttcaat cgcttccagc acactgacat cgtgccacac cggattggtg gtccagtcgc
      181 cgttttcatc caggaaacgg ctgtcctgat tgcgttgcac tgacaattcg acattgccgg
      241 ccggcgcttt ccagccgatc acggtgagca tggcgtagcc ggggccggtg gcggaggctt
      301 tcagttccag tcgtaagggc atgcgttatt ccgtacgggt tgctttgaac gtggcgatga
      361 tgcggcccag cgcatcgttc tgttccggcg tgatttcgcg gcccgcgctg tggccggcgt
      421 tgccgacggc gattttggaa aaggccacca gccagtcgaa caggtaaatg ccggtgtgat
      481 tgatcggttg cggcggcagg tcgggcaggc tgcccggcgc aatcgcggca ggcggctcga
      541 atagtttggc acccttattg gcggcgctgt cgggccgctc ggccagcggc tgctcgatat
      601 agcccagcca ggcaatgaag tccgccagca cggtgcgggc tgccagcacc tgccggtcca
      661 ccagctgttc gcgcttggtg cccacctgct cggtgctgac gatggtatgc agcagccggg
      721 tgcgcaggtc catgcggttg gcggcggtga tcagttcgtc caccagggct tccaccgccg
      781 gtttgggaaa accgaaaaag gccatcaggc gctggtcttc gggaatatta cgcaggtgat
      841 tgatccattc ccgcagcacg gcctgggcaa agcgggcgtc gctgcccacc gccagtgttt
      901 gcgccgtttt ctcgctggcc ttcggcgcgc tgtcgttgct gccgaacaga tccaggtcgt
      961 cgccgagact gatcaggtcg ccacccaggc tgagggcgcc ggcggattcc tggggtgtgc
     1021 cggtttgcgg tgttgatgtg gccaccggtt cgtcatcggc gcgcaggtac agggtacgca
     1081 gcagatcgtc cggcagttgc agccgttgca ggaaatcgcc gagcagcagc gcacgcggct
     1141 tcagcgcatc cacgatctgc tgggcgattt tctttttctt ctccatttcg ccggcacctt
     1201 cggcgagaaa ccaggggccg aggcggtttt ccaccagatc gtgcaccagg tcgtcgagct
     1261 gttcgtggat gcgggccagt ttcacgctgc gcagcgccac cttctgcaga taaccggtga
     1321 cgctgtccat gccgccgtca ttcagcgcca tcatggcatc ccacgcagcg gcaggctcgt
     1381 gcacgtgctc gcgcacggtt tcgtcggcgg cgaaggtgcg tttcatcagc tccagttgcg
     1441 gcgccatggc ggcattcacc gctttttcct cgccgttttc cagatcgatg aaggccacct
     1501 tcatgcgcgg cttgcgcacc aggaaggtgt tgttgaacgg tttgcccggc agccattcct
     1561 gcatccaggc gtactggccg aagcgttcca gcatcgccat cttcatcatg ccgctgttgc
     1621 cccaggcgat ctgcagcaga tcctcaccca tatcgagcga gttgccgatg cgcatgtcga
     1681 acatggtgat ggcccacagc aggccggact ggcgacgggc acggatttcc ggtgtgctgc
     1741 cctgcgtgcg cgcaatccat tcggtgagca ccgggcccac gctggtgaca tccgactgct
     1801 tgttcgacgg cgtgcacacg atcagcacgt tcatttcctg gctgtcggtg tagcgttcga
     1861 acagatacgc caccttgccg cgcaggatca gttgcgccac cgggttggcg tcatcgcttt
     1921 tcaccgcgcg acgcacgtca tccagcgatt ccacgctgag gcggccgcga tagccgggaa
     1981 aatccagcag gtccacatct tcgaacagcg gctcgcgggt gtgttccacc agcgggaaaa
     2041 tcagttcggc ggtaagggcg gccagttgcg ccagcgacaa agtcaccggt ggctgcaatt
     2101 cattgttgac ggagggccac acgctgatgg ttttgtcggt gggtttgccg aggcgctcca
     2161 gcatgtccac gttcatgatg ctgtcggcct gtgacaagcc gccctgcggt gtggcgcgca
     2221 ccagcgcatc cagcggcgcg aacacgctgc cggcgtggcg caggctggcc agggtctggg
     2281 cgaagcgggc gtaggtttcc gtcagttccg gaatttcgcc ccacagaatc gaaaacagtt
     2341 tggcgcggtc gtcgctggac agggacggcg ccagttccac agccgtcggc cagtagtaac
     2401 cgttgagctg cttcagcgaa ccgggaaaac ggtccagcag gtaatcccac agatccacca
     2461 cgtcgtcttc gctgacaccg gcgacaggct ggtgctgttt gcgcttggcc agatccttca
     2521 gcatctggcg cactttggtg tcgttgtatt cgtactggaa tttctcggtg ttgaaatcgt
     2581 tgaagaagga attggccaga atcttcgcca cttccacttc gccaaacagt ttcagttcga
     2641 tggggaaatc cagcggcccc ggttgcgcgg tgcgactgaa gcgggtgacc agtccggtcg
     2701 cttccttgcc gccaccgggc gggttcacat gggtaagaaa atccaggcgc tggccgccga
     2761 aatcggtttc cagtttgccg ttggcgcccg ccgccagtgt tgaaatcaga taggacttgc
     2821 ccgcctgcga caaaccgaaa aagcccactg tcatcggccg gctggacacc gcgcccaggc
     2881 gtcgcgccag attgcgcacg cggcgcattt tcaggatcag gtgatcggct tcgttgtcga
     2941 gccggggcgc attgcggcgc acgtcgctga tccagcgcac ggcatcgccc gcaccggtgt
     3001 gtaccgccgc ccagtgttgg ctgagttgtt cggggctggt tttctgttct gcgccgctca
     3061 tcgaaacacg cttccgctat cgagccagta ttgggattcg cccagtccgg catcggacag
     3121 ggtgttcagt tgcagtttca gcgcccggct gctgacggaa ccggtgtcgg attccacgcc
     3181 ggcgatgatg aaccgctcgg tgccttccgc cggattggcg ctgttgcgct tggcctgcag
     3241 tcgcactttc agtacgcggc cttccgatgc cagcgcttcg ctcagtttct tgtccaccac
     3301 ggtgatggtg tacagtggcg ccgccgacca gcgttccgcc gccagttgcc ggaaacccag
     3361 ccgcatggaa ccgcgcaatt cgaacggcgt gtccggcagt tcgtaatccg gatcgtccag
     3421 attgatcgcc ttgtagtaaa cgttgctttc cttcaccacg ttgttgttgt cgatcagacc
     3481 cagatacttg atggtggaat agggcttgaa cgcggcggcg cggaaaaaga aattcggcag
     3541 gcgcaggctg cggcccagca ggcacagcat ggcgcccacg gcggcggtgg ttttcggatc
     3601 gtcgatgcga ccctgcttgt ggaacggata ccagccgccg gtgcggtaat gatgcagcgg
     3661 caggatgcgc ccgggcggca gcggcagcag actgcggaac accgcctgaa tgcccggcag
     3721 acgcgacggc cggccggtga gcagcagcac gtcgcattga tagcaatgca cgatctcgct
     3781 cagcgccttc agggttttgc agatgttcat gcggttgccg agaaacgcct cgtgcatttt
     3841 gcgcagattc accgcgatcg gcgtttccag aatattgaaa tccgttgcgt tggcgccgag
     3901 ctggcgacgc acggcagcgt tgacgaattc aatcacatcc ggtgtcggcg cctcgtggcc
     3961 cgccagcaga tcagcgaaag taaatgtacc caggctgccc ggttccagcg gatcgtattt
     4021 ttcgtattcc ttcagcaggc gcaggccaat cgggctcaac acctgcagtg ccagttgttg
     4081 ccgcaacacc gcttcctgca ctttcaccgg ctcgttgccg atcaggcgcg acagcagcgg
     4141 ctgcggatca ttcacgccgg ttttcttcag tgcgtcggca aaccagggca ccacgaattt
     4201 ctggatcacc tccagcagga tgtc
//
LOCUS       DASUEG010000754         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_169336, whole genome shotgun sequence.
ACCESSION   DASUEG010000754 DASUEG010000000
VERSION     DASUEG010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169336"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFX11_14265"
     CDS             <1..403
                     /locus_tag="VFX11_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4939838.1"
                     /translation="CKPVPGDEVVGYISVGRGVTVHRKNCKEVTRLMESHADRIVDVS
                     WSAEAGHFYPVDIFIKAYDRQGLLRDITQLLASERVNVTAVNTLSDRSDNTASMALTI
                     EINSLAALGDVLARINQLPNVVEVKRSHQGH"
     gene            451..1293
                     /gene="mazG"
                     /locus_tag="VFX11_14270"
     CDS             451..1293
                     /gene="mazG"
                     /locus_tag="VFX11_14270"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX4939839.1"
                     /translation="MDLASATPIERIRYLMARLRQPETGCPWDAKQTYGTIAPFTIEE
                     AYEVADAIEKGDLDHLKEELGDLLFQVIFYCQMAQEENRFDFNAVARVLEEKLVRRHP
                     HVFPEGTLASRIAPGQQAQDAAGEARIKSNWDAIKLQEKAEKKQLGESILDQVPASMS
                     PLRRAQKIQTVVAKKGFDWEDPFKVMEKIQEELGELQQEMQEAAPVEKIADELGDLMF
                     CCVNLARHYKLDAEMVMTKVNQKFERRFRSLEKALLQQGISLDEATLAQMEAGWQAAK
                     VDEK"
     gene            1324..2319
                     /gene="moaA"
                     /locus_tag="VFX11_14275"
     CDS             1324..2319
                     /gene="moaA"
                     /locus_tag="VFX11_14275"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX4939840.1"
                     /translation="MSNTSALVDKFGRKVEYVRLSVTDRCDFRCVYCMAEEMTFLPRN
                     QILSLEELYRVARVFVQLGVKKIRLTGGEPLVRRDILKLVEKLGQLPGLEQLVLTTNG
                     SQLAHMAQPLKSAGIKRLNISLDSLDPDKFRELTRTGDLQQVLNGIAAAQQAGFKRTK
                     LNAVILKGRNDDEILPLVDFARAQKVDLTFIEEMPLGHISEHGRQECFIPSHEIRDII
                     ATRHELIPSLENSGGPARYYHFRDSDSRIGFISPHSHNFCGDCNRVRVTVEGRLLLCL
                     GNEHSADLRDVLRSSEDDAVLRQAIVAAMDLKPERHHFNLEDEPQIVRFMNMTGG"
     gene            2494..>2623
                     /locus_tag="VFX11_14280"
     CDS             2494..>2623
                     /locus_tag="VFX11_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HEX4939841.1"
                     /translation="MSASSDFIPVNIAVLTVSDTRTLDTDSSGQTLVDGLQSAGHKL"
BASE COUNT          610 a          709 c          796 g          508 t
ORIGIN      
        1 ctgcaaaccg gtgccgggcg acgaagtggt gggttacatt tcggtgggac gcggcgtcac
       61 cgtgcaccgc aagaactgca aggaagtgac gcggctcatg gaatcgcacg ctgatcgcat
      121 cgtcgacgtc agctggtcgg cggaggcggg gcatttctat cccgtggaca ttttcatcaa
      181 agcctatgac cggcaaggac ttttgcgcga tatcacgcag ttgctggcga gcgagcgggt
      241 caacgtcacc gctgtcaata ccctctccga cagaagtgac aataccgcca gcatggcgtt
      301 aactatcgaa atcaacagtc tggccgcctt gggcgacgtg ttggcgcgca tcaaccagtt
      361 gccgaacgtg gtggaagtga aacgttcgca ccaggggcat tgataatgag tgacactgaa
      421 atgagtgcgt cggtgtcaga cattctgcaa atggatcttg ccagtgccac gcccatcgag
      481 cgcatccgct atttgatggc gcgcctgcgg caaccggaaa ccggctgccc ctgggacgca
      541 aaacagacgt acggcaccat tgcgcccttc accatcgagg aagcctatga agtggcggat
      601 gccatcgaaa aaggcgatct ggatcatctc aaggaagaac tgggtgattt gctgttccag
      661 gtgatttttt actgtcagat ggcgcaggaa gaaaaccgct tcgatttcaa tgcagtggcg
      721 cgggtgctgg aagaaaagct ggtgcggcgg catccccatg tgttccccga gggaaccctg
      781 gcgagccgga tcgcgccggg ccagcaggcg caggatgctg ccggagaagc ccggataaaa
      841 tccaactggg acgcgatcaa actgcaggaa aaggcagaga aaaagcagct gggtgaatcg
      901 attctggatc aggtgcctgc cagcatgtca ccgctgcggc gagcccagaa gatccagact
      961 gtggttgcca agaaaggctt cgactgggaa gatccgttca aggtaatgga gaaaatccag
     1021 gaagaactgg gtgaactgca acaggaaatg caggaagcgg cgccggtgga aaaaattgcc
     1081 gacgagctgg gcgacctgat gttctgctgc gtgaatctgg cgcggcatta caagctggac
     1141 gcggaaatgg tgatgacgaa agtgaaccag aaattcgaac gccgtttccg ttcgctggaa
     1201 aaggcgctgc tgcaacaggg catctcgctg gacgaggcga ccctggcgca aatggaagcc
     1261 ggctggcagg ccgccaaagt ggatgagaag taacggggca atttgagcag acaggttgaa
     1321 accatgagca acacatcagc attggtcgac aaatttggac gcaaggtgga atacgtgcgt
     1381 ctgtcggtca ctgatcgctg tgatttccgt tgcgtgtact gcatggcgga ggaaatgacc
     1441 ttcctgccgc gcaaccagat cctgtcgctg gaagaactgt accgggtggc ccgtgttttc
     1501 gtgcagctcg gcgtgaaaaa aatccgcctg accggtggcg aaccgctggt gcggcgcgac
     1561 atcctgaaac tggttgaaaa gctgggtcag ctgccgggcc tggagcaact ggtgctcacc
     1621 accaacggtt cccaactggc gcatatggcg caaccgctga agtcagccgg tatcaaacgg
     1681 ctcaacatca gtttggacag tctcgatcct gataaatttc gcgaactcac ccgcaccggc
     1741 gatctgcagc aggtattgaa cggcatcgcc gccgcgcagc aggccggttt caaacgcacc
     1801 aaactcaacg ccgtgattct gaaaggccgc aacgacgatg agatcctgcc gctggtggat
     1861 ttcgcccgtg cacagaaagt ggatctgacc tttatcgagg aaatgccgct gggccatatt
     1921 tcggagcacg gccgccagga atgcttcatt ccctctcacg aaattcgcga catcattgcc
     1981 acccgtcatg aattgatacc gtcgctggag aacagcggcg gccccgcgcg ttattatcat
     2041 ttccgcgaca gcgattcccg catcggtttc atctcacccc acagccacaa tttctgcggc
     2101 gactgcaacc gggtgcgggt gacggtggag gggcggttgc tgctgtgtct gggcaacgaa
     2161 cattccgccg atctgcgtga tgtgttgcgc agcagtgagg atgatgcggt gctgcggcag
     2221 gcgattgtcg cggcgatgga tctgaaacct gaacgtcatc atttcaatct ggaagacgaa
     2281 ccgcagatcg tgcggttcat gaatatgacg ggcgggtgaa ttgttttaga agagtggcac
     2341 attgtcggaa ttccgggcgg tctgccgttc gagaccgtca gaggcccggg atggcctctg
     2401 acgagcttac agggatgtac ttgcagcgtg tctcgaacgg cagaccgccc ggaattcgaa
     2461 cgtggcacaa tcgttgaaat tccgaggaac cccatgagcg catcatccga tttcattccc
     2521 gtcaacatcg ccgtactcac cgtctccgac acccgcacgc tggacaccga cagctccggc
     2581 cagactctcg ttgacgggct tcagtccgcc ggccacaaac tcg
//
LOCUS       DASUEG010000755         5439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2180609, whole genome shotgun sequence.
ACCESSION   DASUEG010000755 DASUEG010000000
VERSION     DASUEG010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180609"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1366)
                     /locus_tag="VFX11_14285"
     CDS             complement(26..1366)
                     /locus_tag="VFX11_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939842.1"
                     /translation="MKQKLKWAGLGAALILAACGAKFWPAQWSAQYAVQDSQVATSQS
                     VSRELADGSIRVAYRRDDDTLVVDTYDTTGLALQQDEFQLPFPSHFQFGVAPLFLDDN
                     LLVWFGRNDRDSVLIDVATQTVASLHEVLALDLPLDISFEIADAVVLDNGQLVLVGGD
                     RRSPTSPFRQARVAVMHPGGSVESSVLAVGTSFSNVVARSGSDQYFVQYFEFDTTVPA
                     TVAFTFLGEGSVLMEQPQIDRGSLLQADLAGAWASGVFAAGLLHYNADLVLDWQDLSI
                     LVRKLVSTSDQHYLMLQLIDEQMAVVKRDAQAAVVWQRPASADATIFGGLAERDGRIL
                     YSESFSSRVKSVRTLPDDPAEGLPITTVAEKVRHRILDANGKELHRFKEPDYKAVLPR
                     DPFTDLEGDALEYTAGSCQHFQGMLLAGGQFASVSRWCGDNGLDNQQESVFYFQ"
     gene            complement(1535..2167)
                     /locus_tag="VFX11_14290"
     CDS             complement(1535..2167)
                     /locus_tag="VFX11_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX4939843.1"
                     /translation="MNPAPARTLAIVSAILIAALVVSGIDPYDFATWLLEVFPVLVAL
                     PLMWFTLRRFPLTTLLYIGICLHSLVLILGGTYTYARVPLGFQLAEWFDLSRNPYDKI
                     GHFFQGFVPALVAREILIRGRYVNGRGMLAFIVVSIVMFISSFYELIEWWVALAIGQG
                     ATEFLGTQGYEWDTQSDMFLALIGAVCALLTLARWHDRQIAQLPSGPKSA"
     gene            complement(2275..2907)
                     /locus_tag="VFX11_14295"
     CDS             complement(2275..2907)
                     /locus_tag="VFX11_14295"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX4939844.1"
                     /translation="MRLIGMLDSPYVRRVAITLDCMGIPFRHEAVSVFSTFEQFQRIN
                     PVVKAPSLVCDDGEVLMDSSLMLQYAAALPACRDVLLPQTLPELQHALRAIGLALAAC
                     EKSVQIVYERNLRPLEKQHAPWLERVQGQLLAAYAGLEQEVKRQTSLFDGSLNQASIT
                     AAVAWQFTQSMLASSVEATPFPALQKLCIALEASPVFLRYPADGPGVVAN"
     gene            complement(2920..3876)
                     /locus_tag="VFX11_14300"
     CDS             complement(2920..3876)
                     /locus_tag="VFX11_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX4939845.1"
                     /translation="MAKKSDIAWSFIGIAAVVVSCFLLYKQVHKISLDELAGSLRAIP
                     LDNWLLAMGATLGAYGALAWYDRIALAHLGKRVSWWFIALCSFTTYALAHNIGASVFS
                     GALVRYRAYSSKGLSPQEIGIVIVFCAFTFALGTVLAGGVVLIAEPHLIDRITPLNPW
                     LSVLAGSFLLLLVTLYVLGSWRHFKPWTWGKFHVEYPRLGIVARQLLAGPLELLCAAA
                     IIYFSLPEANHPGYMVVLGVFLASFSLALLSHAPGGLGVLEVTFIAALPEIPTVDVLA
                     ALIVFRCFYLLLPFALSLFVVSGFEWTHWRERSRPPSDPEQP"
     gene            complement(4006..>5439)
                     /locus_tag="VFX11_14305"
     CDS             complement(4006..>5439)
                     /locus_tag="VFX11_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4939846.1"
                     /translation="AIAAFLLNGALRGWLFKANLAGKPLPYLVTRIDFTPPGEEESGR
                     VLLELKANAMGKVITQTLTILERDIADKTLAELFASKGLLKETPALMEHYEQSSARYF
                     DWRARYGHQFSGRGTAIFAEDPTASHRTTDWSRKEKVVLSSGGGWARLVNDEDILKDR
                     DLTLDAGGDILGKYLRKAGRSANYSDQVERAVANVKADIPRGLFSEMPVHGYILLFHL
                     ELHHHVWVHVDDLQPYAYQPDLKQKLILPAEQTDLIDILTAEMDVLMDDIVAGKSGGT
                     TVLCAGPAGVGKTLTAEVYAEIIQRPLYRVHSGQLGLNVTQMEQALKDTLTRAQRWGA
                     VMLIDEADVYIRRRDDNMAANAVVGVFLRVLEYFNGLLFLTTNRVNDIDEAIVSRCIA
                     LIRYHPPQLADRIRIWQVMMEQFGLAVTPDMPDVLARRFPDATGRDIKGLAKLVAKYC
                     QHKAVTPSLPVFERCSLFRGMESAGAD"
BASE COUNT         1170 a         1710 c         1623 g          936 t
ORIGIN      
        1 aatccgcgcc tattgaaaac cacacttact gaaaataaaa caccgactcc tgctggttat
       61 ccaatccatt atcgccacac cagcgcgaca ccgacgcaaa ctggccaccg gccagcagca
      121 tgccctgaaa atgctggcaa ctgccggcgg tatactccag cgcatcaccc tcaagatcag
      181 taaatggatc gcgcggtaat actgccttgt aatccggctc cttgaagcgg tgcagctcct
      241 tgccatttgc gtccagaatg cgatgccgca ctttttccgc aacggtggta atcggcaaac
      301 cttcggccgg atcgtccggc agtgtgcgta cgctcttcac ccggctgctg aaggattccg
      361 aatacagaat ccgcccatcc cgttccgcca gaccgccgaa gatggtggca tcagccgacg
      421 ccggacgctg ccagaccacc gcggcctgcg cgtccctttt cactactgcc atctgctcgt
      481 cgatcagttg cagcatcagg taatgctgat ccgacgtgga caccagtttg cgtaccagga
      541 tgctgagatc ctgccagtcc agcaccagat ccgcgttgta atgcagcaga ccggcagcga
      601 aaacccccga tgcccaggcg cccgcaagat cggcctgcag caacgatccc ctgtctatct
      661 gcggctgctc catcaacaca ctgccttcgc ccagaaaagt aaaggcgacg gttgccggaa
      721 ccgtcgtatc gaattcaaaa tactgtacga aatactgatc cgatccggaa cgcgccacca
      781 cattggaaaa gctggtgccc accgccagca cgctggattc cacgctaccg ccaggatgca
      841 tcacggccac acgggcctga cggaacggcg atgtcgggga tctgcgatcg ccgcccacca
      901 gcaccagctg cccattgtcg agcaccacag catcggcaat ctcgaagctg atatcgagcg
      961 gcaaatccaa cgccagcacc tcgtgcaagc tggcaaccgt ctgcgtcgcc acgtctatca
     1021 gcacagagtc gcgatcgttt ctgccgaacc acaccagcag gttgtcatcc agaaacaggg
     1081 gggctacgcc gaactggaaa tggctgggga atggcagctg gaattcgtcc tgctgcagcg
     1141 ccaatcccgt agtgtcgtaa gtgtccacca ccagagtatc gtcatcgcga cggtaagcga
     1201 cgcggatcga gccatctgcc agttcacgac tcaccgattg cgacgtggca acctggctat
     1261 cctgcactgc atattgcgca ctccactgag ccggccagaa ttttgctccg catgcagcca
     1321 gtatcagagc cgcgccaagg cccgcccatt tgagtttttg cttcatggaa tcgcgtcctg
     1381 ttctgccttt gattgaaggt tctggcaccg ttgtacccgg tgaacggtgg aatcgactct
     1441 agcacggagc cggcgcagga ctgaaggctg ctcgcatttt ataaaaagat aaagcgacgc
     1501 acggtttgca gggcgcgccg ctcccgggac atggctaggc cgacttcggg ccggatggca
     1561 gttgcgcaat ctggcgatca tgccagcgcg ccagtgtcag caacgcacac acggcgccaa
     1621 tgagcgccag gaacatatcg gactgtgtat cccactcata accctgggtg ccgagaaatt
     1681 ccgtcgcgcc ctgaccgatg gccagcgcca cccaccattc gatcagttcg tagaaggagc
     1741 tgatgaacat cacgatgctc accacaatga aggccagcat gccgcggcca ttgacgtagc
     1801 ggccgcgaat cagaatttcc cgcgccacca gcgccggcac aaaaccctga aaaaagtggc
     1861 cgatcttgtc gtaggggttg cggctcagat caaaccattc tgccagctga aaacccagcg
     1921 gcacgcgggc atacgtgtag gtgccgccca ggatcagcac cagcgagtgc aggcagatgc
     1981 cgatgtacag cagcgtggtc agcggaaaac gtcgcagcgt aaaccacatc agtggcaatg
     2041 ccaccagaac cggaaacacc tccagcagcc aggtggcaaa atcataggga tcgatgccgg
     2101 agacgaccag ggctgcaatc agaatggccg agacaatagc gagcgtgcgt gcgggtgcgg
     2161 gattcatggg ccgggccttt tttgccaatg caactgtcgc atagtagcac cgaccacgtt
     2221 cgccgcctga gggggcgggg cgacgccatg ctttagatca aaaaaatgcg ggagtcagtt
     2281 cgctaccaca cccggcccat ccgccggata gcgcagaaaa acgggcgagg cctccagcgc
     2341 gatgcacagt ttttgcaggg cgggaaatgg cgtggcctcg acactggagg ccagcatgga
     2401 ctgggtgaac tgccaggcca ccgccgcagt gatggaggcc tggttcagac tgccatcgaa
     2461 cagcgaggtc tggcgcttca cttcctgctc cagtccagca taggccgcca gcaattggcc
     2521 ctgcacacgc tcaagccagg gagcgtgctg tttttccagc gggcgcaggt tgcgctcgta
     2581 cacgatctgc acacttttct cgcaggcagc cagcgccagg ccaatcgcgc gcagggcgtg
     2641 ctgcagttcc ggcagggtct gcggcaacag cacatcgcga caggccggca gtgcagcggc
     2701 atattgcaac atcaggctgg aatccatcag cacctcgccg tcgtcgcaca ccagcgaagg
     2761 cgctttcacc accggattga tgcgctggaa ttgctcgaag gtgctgaaca ccgatacggc
     2821 ttcatgccgg aacgggatgc ccatgcagtc cagggtgatg gcaacgcggc ggacataggg
     2881 tgaatccagc attccgatca ggcgcatgtg gcgctccttt cagggttgtt ccgggtcgga
     2941 cggcggtcgc gaacgttccc gccagtgggt ccactcgaaa ccggacacca caaacagcga
     3001 aagcgcaaac ggcagcagca ggtaaaaaca gcggaatacg atcagcgccg ccagcacatc
     3061 cacggtggga atctccggca gcgcggcgat gaacgtcact tccagcaccc ccaggccacc
     3121 gggcgcatgg gacagcagtg ccaggctgaa ggacgcaagg aacacaccca gcaccaccat
     3181 atagcccgga tgattggcct cgggcagcga gaagtagatg atggccgccg cgcacagtaa
     3241 ttccaacggg ccggccagca gttgccgggc aacaataccg agccgcgggt attccacatg
     3301 gaacttgccc caggtccagg gcttgaagtg gcgccaggaa ccgagcacat acagggtgac
     3361 caggagcagc aggaaggagc cggcgaggac cgataaccag ggattcaggg gcgtgatgcg
     3421 gtcgatcaga tggggttcgg caatcaggac cacgccaccc gccagcacgg tgcccagggc
     3481 aaaggtgaag gcgcagaaca cgatgacaat gccaatttcc tgcggcgaca accccttgct
     3541 gctgtaggca cggtagcgca ccagcgcgcc ggagaacacg ctggcgccaa tgttgtgcgc
     3601 aagggcatag gtggtgaagg aacagagggc aatgaaccac caggacacac gcttgcccag
     3661 atgcgccaga gcgatgcggt cataccaggc cagagcgcca taggccccca gggtcgcgcc
     3721 catggccagc agccagttgt cgagggggat cgcgcgcagg ctgcccgcca gctcgtccag
     3781 cgagattttg tgcacctgtt tgtagagcag gaagcaggaa acgacgacgg cggcaatgcc
     3841 aatgaagctc caggcgatgt cgctcttttt ggccatgaaa gaatccttac agcgctggca
     3901 ggggcatcct gccgacaggc ccggcgcaat gcgcccaggg acacctgatg gataacggca
     3961 cgtagtatcc ctgacgtcat ggctttgcac aatggggcgc ccgtctcagt cagcgccggc
     4021 ggactccatg ccccggaaca ggctgcaacg ttcgaacacc ggcaggctgg gtgttaccgc
     4081 cttgtgctgg cagtacttgg ccaccagttt ggccagcccc ttgatgtcac ggccggtagc
     4141 atcgggaaaa cgccgggcca gcacatccgg catatccggc gtcaccgcca ggccgaactg
     4201 ctccatcatc acctgccaga tgcggatgcg gtctgccagc tgcggcggat ggtagcggat
     4261 cagggcgatg cagcgcgaca cgatggcctc gtcgatatcg ttcacccggt tggtggtgag
     4321 aaacagcagc ccgttgaaat attccagcac ccgcaggaac acgcccacca ccgcgttggc
     4381 ggccatgttg tcgtcgcggc gacggatgta gacatcggcc tcgtcgatca gcatcaccgc
     4441 gccccagcgc tgggcccggg tcagggtgtc cttcagggcc tgctccatct gcgtcacatt
     4501 caggcccagc tggccggagt gcacgcggta cagcgggcgc tggatgattt ccgcatacac
     4561 ctccgccgtc agcgttttgc ccacgccggc gggccccgcg cacagcaccg tggtgccacc
     4621 ggatttgccg gccacgatat cgtccatcag cacatccatc tcggcggtga gaatgtcgat
     4681 caggtcggtc tgctcggccg gcaggatcag tttctgcttc aggtccggct ggtaggcata
     4741 gggttgcagg tcgtccacgt gcacccagac gtggtgatgc agctccagat gaaacagcag
     4801 gatgtagccg tgcaccggca tttccgagaa cagcccgcgg ggaatgtcag ccttcacgtt
     4861 ggccacggcg cgctccacct ggtcgctgta attcgcactg cgtcccgcct tgcgcaggta
     4921 tttgccgagg atgtcaccac cggcatccag ggtgagatcg cggtctttca gaatgtcttc
     4981 atcattcacc agccgggccc agccgccgcc ggatgacagc accacctttt ccttgcggga
     5041 ccagtcggtg gtgcgatggc tggcggtggg gtcttccgcg aagatggcgg tgccgcgccc
     5101 ggagaactgg tggccgtaac gggcacgcca gtcgaaatag cgggccgatg actgctcgta
     5161 gtgttccatc agcgccgggg tttccttcaa caggcccttg ctggcgaaca gttctgccag
     5221 ggttttgtcg gcgatgtcgc gctccagaat ggtcagtgtc tgcgtgatca ccttgcccat
     5281 ggcattggcc ttgagctcca gcagcacgcg gccggattct tcttcacccg gcggcgtgaa
     5341 gtcgatgcgc gtcaccagat agggcagcgg cttgcccgcc agattggcct tgaacagcca
     5401 gccccgcagc gcgccattca gcaggaacgc cgcgatggc
//
LOCUS       DASUEG010000756         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1179944, whole genome shotgun sequence.
ACCESSION   DASUEG010000756 DASUEG010000000
VERSION     DASUEG010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179944"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..2384)
                     /locus_tag="VFX11_14310"
     CDS             complement(252..2384)
                     /locus_tag="VFX11_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX4939847.1"
                     /translation="MDCPTEEKLLRDKLSRVPGVQALEFNLMQRVLVVNQLPEAQAAV
                     IAGIRELGFTPELLADSNTAAQDPNVSAEPTPGTGRSQWVLLALSGVTAVGAEAVEWL
                     NAPHWLAGALALVAIASVGLNTYKKGWIALKNRNLNINALMSIAVTGAMLIGEWPEAA
                     MVMFLFTVAELIEARSLDRARNAIRGLLELAPEQARVRQDDGQWRTQPSKAVAVGAIV
                     QVLPGERIGLDGEVTDGRSAVNQAPITGESLPVEKGIGDPLFAGTINQSGVLEYRVTA
                     LVGDSTLARIIHAVEEAQGARSPTQRFVDQFSRIYTPLVFVLALLVAVVPPLYLAPAD
                     GQIWLDWIYRALVLLVIACPCALVISTPVTIVSGLATAARRGILVKGGTYLEMGRDLQ
                     VLALDKTGTLTFGKPVQTDQFILVGEAKDVQILAVTLARHSHHPVSKAIALAESAEGI
                     PSRSLAEFEDLPGRGVRGRIDGALHHLGNHRLIEELGLCSPALEQRLEQLEQQGKTVV
                     MLTDERQVLALFAVADSVRPASAAAIEALKALGIRTLMLTGDNPHTAQAIAAQVGIDA
                     VHANLLPQDKLQRVSELVDTGSVVGMVGDGINDAPALARANIGFAMGAAGSDTAIETA
                     DVALMDDDLRKIPEFVRLSRKTAAVLKQNIGIALGIKLVFLVLTLLGSATLWMAVFAD
                     LGASLIVIANGLRLLKTSNGQLSGSQES"
     gene            complement(2551..>4001)
                     /locus_tag="VFX11_14315"
     CDS             complement(2551..>4001)
                     /locus_tag="VFX11_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4939848.1"
                     /translation="LDEGDLLYMPSALPGLSAAKASELLQISDRLIKTVPEVDTVFGK
                     AGRAETATDPAPLEMFETTIRFKPREQWRPDMTPEKLVEELDRVVRVPGLTNIWVPPI
                     RNRIDMLATGIKSPIGVKISGTDLAAIDRIAAQVELAAKTVPGVSSALAERLTGGRYI
                     DVQINRTRSARYGLNIADVQAVVSSAIGGENIGETVEGLARFPINVRYPREWRDSLER
                     LRQLPILTPQGQQITLGSVADLKISDGPPMLKSENARLSGWVYVDVRGRDLASTVRDL
                     QAAVARDVALEPGMSLSYSGQFEFLERANAKLQQVVPATLLIIFVLLYLTFRNVAEAL
                     LILATLPFALTGGVWFLYGMGFNLSVATGVGFIALAGVAAEFGVVMLLYLKQAIASRE
                     ARGQALTVAVLDNAIREGAVLRVRPKAMTVAVILAGLLPILLGDGAGSEIMSRIAAPM
                     VGGMLTAPLLSMLVIPVGYLLLRQQRAVDGKS"
BASE COUNT          811 a         1251 c         1127 g          812 t
ORIGIN      
        1 gcatttgctt ttgcatgaca tccatatgct cctgcatttt tttcagatct gcatcggtca
       61 ttccggttgt gggttcggct gccattacag gccctgccaa gccaatcagc gaggcgagga
      121 taataggacg gatatgtttc attgacatgg ttgcgttctc ctttggttgt gttggcgcaa
      181 gccgcgctgg acctgcactt gtatagcctc agaagcggtg cttctgcggt aattcatgaa
      241 tccctacgct atcatgattc ctgtgagcca ctcagctgtc cgttcgacgt tttcagcaag
      301 cgcagtccat tagcaatgac gatcaggctg gcccccaagt ccgcgaacac ggccatccag
      361 agcgttgccg atccaagcag ggtcaacacc agaaacacca gtttgatacc cagagcgatg
      421 ccgatgttct gtttcagcac cgccgccgtt ttacgtgaca atcgcacaaa ttcaggaatc
      481 ttgcgcaggt cgtcgtccat cagggcgacg tcggcggttt cgatcgcggt gtcgctgcca
      541 gcggcaccca tggcgaagcc aatgttggct cgggccagcg cgggcgcatc gttgattccg
      601 tctcccacca tgcccaccac cgaacccgtg tccaccagct ccgacacccg ctgcagtttg
      661 tcctgtggca acagattggc gtgcacggcg tcgatgccca cctgagctgc gattgcctgc
      721 gcggtatgag gattgtcacc ggtcagcatc agggtgcgaa tacccaatgc tttcaacgcc
      781 tcaatggcgg cagcgctggc tgggcgaaca ctgtcagcca cggcgaacag ggccagcacc
      841 tgacgctcat cggtgagcat caccactgtc ttgccttgct gttcaagttg ctccagccgt
      901 tgctccaagg caggtgaaca caagcccagt tcttcgatca gccgatggtt gccgagatgg
      961 tgcaacgcac catcgatgcg accgcgcaca cctcgacccg gaagatcctc gaattcagcc
     1021 agactgcgcg atggaatgcc ctccgcagat tccgccagag cgattgcttt cgacactgga
     1081 tggtgagaat gccgcgcaag cgtgacagcg agtatctgca catcctttgc ctcgcccacc
     1141 agaataaact ggtcggtttg gactggcttg ccaaaggtca aggttccggt tttgtccagc
     1201 gccagcacct gcaagtctcg tcccatttcc agataagtac cgcccttgac cagaatgcct
     1261 cgccgggccg ctgtcgccag tccgctcaca atggtcacgg gtgtggatat caccaaggca
     1321 cagggacagg cgatcacgag caataccagt gcacggtaaa tccagtcgag ccagatttgg
     1381 ccatcagctg gtgcgaggta aagaggtggc accaccgcca ccagcagcgc cagcacaaag
     1441 accagcggcg tgtagatgcg ggagaactgg tcgacgaagc gctgtgtggg ggagcgagcg
     1501 ccctgggctt cttccactgc gtgaatgatg cgggccaggg tggaatcgcc tactaacgct
     1561 gtaacacggt attccagcac cccagattga ttgatggtgc ccgcgaaaag tgggtcgccg
     1621 atgccttttt ccacgggcag gctttcaccg gtgatcggtg cctgattcac ggcggaacgc
     1681 ccgtccgtca cttcgccatc cagcccaata cgttcaccgg gcagtacctg cacgatggcc
     1741 cctaccgcta ctgccttgct gggctgtgtc ctccactggc catcgtcctg acgcacgcgg
     1801 gcttgctcgg gggccagttc cagcaggccg cgtatcgcat tgcgggcgcg gtccagcgag
     1861 cgggcctcga tcaactccgc cactgtgaac aggaacatca ccatcgccgc ttccggccac
     1921 tcgccaatca gcatggcacc ggtaacggca atactcatca gtgcgttgat gttcagatta
     1981 cggtttttga gggcaatcca gccttttttg taggtgttca atcccacgct ggcaatggcc
     2041 accagtgcga gcgcccccgc cagccagtgc ggtgcgttca accattccac tgcttcggct
     2101 cccacggccg tcacgccgga caaggccagc aatacccatt gagagcggcc ggtgccgggc
     2161 gtcggctccg cgctgacatt tgggtcctgc gcggcggtgt ttgagtccgc cagcagttcc
     2221 ggtgtaaatc ccaattcgcg aatccctgcg atcacggcag cctgcgcctc cggcagctga
     2281 ttgacgacca gtacacgctg catcaagttg aattccaggg cctgcacacc aggcacccgc
     2341 gacaatttgt cgcgcagcaa cttttcctcg gtgggacagt ccatttgggc gatgcgatag
     2401 cggacccccg tacctggtgg cgccggctcg gcagggacgg gggaacgagt cgtgttgcag
     2461 gaactggaag gatcgcagca gtttgacatg atatgtcctt gtcacttgaa cgtggttaac
     2521 aacagttaac acctggagcc tgctccagag tcaagactta ccatcgacag cccgttgttg
     2581 ccgtaacagc aaatacccga ccggtatgac cagcatcgac agcagcggcg cagtcagcat
     2641 gccacccacc atgggcgctg cgatccggct catgatttcc gagccggcgc catcccccag
     2701 caaaatgggc aacaggcctg ccaggatcac tgccaccgtc attgccttcg gtcgcactct
     2761 tagtacagct ccctcccgga tggcattgtc cagcactgct acggtcagcg cctgaccacg
     2821 agcctcgcgg ctggctatcg cctgtttcag atagagcaac atcaccacac caaactccgc
     2881 agcaacacct gccagcgcaa taaagcccac gcccgtggcc accgataagt tgaagcccat
     2941 gccatagaga aaccagacgc cgccggtcag ggcaaatggc agggttgcca ggatcaacag
     3001 ggcttcagcg acattgcgaa atgtcaggta caacaggaca aagatgatca gcaaggttgc
     3061 cgggacaacc tgctggagtt tggcattggc ccgctccaga aactcgaatt gtcccgagta
     3121 ggaaaggctc atgcccggct ccagtgccac gtcgcgtgca acggcggcct ggagatcgcg
     3181 cacggttgaa gccagatcgc gaccccgcac atccacgtac acccagcccg acaggcgcgc
     3241 attttcactt ttcagcatcg gcggaccatc gcttatcttc aggtcggcaa cgctgcccag
     3301 tgtgatttgc tggccttgcg gcgtcagaat tggcaattgc cgcagccgtt ccagcgagtc
     3361 ccgccattcg cgcggatatc gcacattgat gggaaagcgg gccaatcctt ccactgtttc
     3421 accgatattc tcgccgccga tggcggaact caccacggcc tgcacatcgg cgatattcag
     3481 gccgtagcgg gccgaacgcg tacgattgat ttgcacatcg atatagcgcc caccggtcag
     3541 gcgttccgcc agcgcggagc tgacaccggg aaccgttttc gctgccagct ccacctgtgc
     3601 tgcaatgcga tcgatggccg ccagatcggt acctgaaatt ttgacgccga tcggactttt
     3661 gatgccggtc gccaacatgt cgatgcgatt gcggatggga ggcacccaga tattggtcag
     3721 tcccggcacg cggaccacac gatccagttc ctcgacgagt ttttccggtg tcatgtcggg
     3781 ccgccactgt tcccgtggct tgaaacggat cgtggtttcg aacatttcca gaggcgcggg
     3841 gtcggtcgcc gtttcggcgc ggcccgcctt gccgaacacg gtgtccacct ctggtacggt
     3901 tttgatcagg cggtcggaga tttgcagcag ttcagaggct tttgcggccg acagtcccgg
     3961 cagcgcagag ggcatgtaca acagatcccc ttcgtccaat g
//
LOCUS       DASUEG010000757         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1092644, whole genome shotgun sequence.
ACCESSION   DASUEG010000757 DASUEG010000000
VERSION     DASUEG010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092644"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1526)
                     /gene="mltF"
                     /locus_tag="VFX11_14320"
     CDS             complement(150..1526)
                     /gene="mltF"
                     /locus_tag="VFX11_14320"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEX4939849.1"
                     /translation="MRGAFLGALLFTLFHLKPATTALQQVQQRGTLILAGTSGPTTFQ
                     ETVDGARGFQYELAQLFAEELGVELVLDDTPDTNGVLSAIRRNKADIAITGLATDDPR
                     LKRLRVAEPYMAVSEQLIQRLDKPIPARFDDLENARIGVIAGSTEAVRLQSMTRWRPD
                     LSVIEFEDTDPFLLLDQVAAGELDYVAMNSSEFDARRALFPNLGVSLNLQDNSALAWA
                     FIKTQDQTLFRAAQAFLARKQADGTLDRLVAFYSQGDTFDPYSVRSFQRDITQKLPRY
                     EGLFRKNADAQSMDWRLLAAIAYQESRWQPNATSPTGVRGMMMLTQGTASHMGVSDRT
                     NARQSIEGGAGYYRQILERLPDTVREPDRTWMALAAYNMGPAHIDRARQSATDHGEDP
                     NKWLTISRHLRMMAANARKQGRRIPVGQALVYVQQVRRYYDALQLATTFSSDMRVAMN
                     QNERDITR"
BASE COUNT          398 a          616 c          699 g          451 t
ORIGIN      
        1 aacaggatgt ccttgatgac gttcgggcag gcatctgcat ccacccagag ttgcagttgt
       61 cgcgtctccg tcatgtcagg tccgctcctt tcgtgcaggc aaaaaaatcc ccggcgcccc
      121 ggggaatgag agagaaaaga cgggcatttt tagcgagtga tgtcgcgctc gttctgattc
      181 atggccacgc gcatgtcact gctgaaggtg gtggccagtt gcagtgcgtc ataataacgg
      241 cgaacttgct gaacatagac cagcgcctgg ccgaccggaa tgcgacgtcc ctgcttgcgg
      301 gcgttggcgg ccatcatgcg caggtgacgg ctgatggtca gccacttgtt cgggtcttcg
      361 ccgtggtcgg tggcgctctg acgagcccga tcaatgtggg ccgggcccat gttgtaagcg
      421 gccaatgcca tccaggtgcg gtccggctcg cgcaccgtat ccggcaggcg ctccagtatc
      481 tggcgatagt aaccagcgcc gccctcgatg ctctggcgtg cattggtacg gtccgatacg
      541 cccatgtgag aggcggtgcc ctgggtcagc atcatcatgc cgcgcacacc agtgggtgag
      601 gtggcgttgg gttgccaccg ggattcctgg taggcaatgg cggccagcag gcgccagtcc
      661 atgctctggg catcggcatt cttgcggaag agtccctcgt agcggggaag cttctgggtg
      721 atgtctcgct ggaagctgcg aaccgagtag ggatcaaagg tatcgccctg actgtagaag
      781 gctacaaggc ggtccagcgt gccatctgcc tgcttgcgag ccagaaaggc ctgggcggca
      841 cggaacaggg tctggtcctg cgtcttgatg aatgcccagg caagagcgct gttgtcctgg
      901 aggttcagcg acacgccaag attggggaac agggcgcgac gggcgtcgaa ctcgctggag
      961 ttcatggcaa cataatccag ctcgccggca gcaacctggt ccagcagtag gaagggatcc
     1021 gtatcctcga actcgatcac cgacagatcc ggacgccacc gtgtcatgga ctggaggcgg
     1081 acggcctctg tcgagccggc gatcacgccg atgcgggcat tttcaaggtc gtcaaagcgg
     1141 gccgggatcg gcttgtcgag gcgctggatc agctgttcgc tgacagccat gtagggctcg
     1201 gcgacccgca gccgcttcag gcgggggtcg tcagtagcca gtccggtgat ggcaatgtct
     1261 gccttgttgc ggcggatggc gctcaggacg ccgttggtgt caggagtatc atccagaacc
     1321 agctctaccc ccagctcctc agcaaagagc tgggccagct cgtactggaa gccacgagcg
     1381 ccgtcgacag tttcctgaaa agtggtcggg ccgctggtgc cggcaagaat gagcgtgccg
     1441 cgctgctgaa cctgctgcaa ggcggtggtg gccggcttca ggtgaaacag ggtgaacagg
     1501 agtgcgccca aaaaggcacc gcgcatcagg agcttgagtc cttttacggg gtggggcagt
     1561 cggtctcgta gcgcctggaa gccgctagac atccttagta cctcgttcgg tttaccgttt
     1621 attccagttg tctgcatccg ctgcagaaca gccgtcgtcg cgtttaccct agggggtgtt
     1681 tcggtagcca gtgggctcac caaagcgcgt tgccgaacct gccggagcag ggcctcaacg
     1741 cgaggtcggg cgaaatctat cgaccggttc acactatgtc tagtccttga cagcagattt
     1801 tgcccgagat tgtccgacaa taatttggtg acctgaccga tgaaagcatt gtccgggtgt
     1861 cctgaaaccc ccgattccag tatgattgcg gcccttcccg ggccggtttt gccgtccgtc
     1921 gtgtgactga ccgttcagcg actgaccccg ggtgattccc cctcatttcg ccgttttccg
     1981 agagaccgac atgctcatcc tgcctggttc cctggcgctt tccgctttcc gacagaaccg
     2041 actgctgcgc gaccttaagg ccatgttgcc caaggtgaca gatgtgcatg cgcggtttgt
     2101 gcacctggtg gatggtcagc ctgatgagga aggattgtgt cggctggagg agctgctgca
     2161 ctac
//
LOCUS       DASUEG010000758         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1596915, whole genome shotgun sequence.
ACCESSION   DASUEG010000758 DASUEG010000000
VERSION     DASUEG010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596915"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFX11_14325"
     CDS             complement(<1..708)
                     /locus_tag="VFX11_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4939850.1"
                     /translation="MKYVRHVFGLMAVLGLAACSDEGSKTAASAELIVKVGHAAPMTG
                     PQAHLGKDNENGVRLAIDELNANPPVIGGKPVKFELLSEDDQADPRIATTVAQKFVDE
                     KVHAIIGHLNSGTTIPASRIYADAKIPQISPSATNVRYTQQGFKNAFRVMANDAQQGS
                     VLGKFAVGEMKAKTIAIIDDRTAYGQGLADEFEKAAVAGGATIVGHEFTTDKSTDFTA
                     ILTKIKGLNADLLFYGGM"
     gene            complement(949..>2122)
                     /locus_tag="VFX11_14330"
     CDS             complement(949..>2122)
                     /locus_tag="VFX11_14330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4939851.1"
                     /translation="WSMILLSTLVCIGVSHAYVRSAASFREEWYLLLLVSACGGMVLA
                     ASSHMAALFIGLELMSVPLYGLAAFALKNKRSLEAGIKYLVLSATASAFLLFGIALIY
                     AETGSLLLAQWASLPVDSSPGLLGCAMILIGLGFKLSWVPFHTWTPDVYQGAPMPVTA
                     FLATASKVAVVAVLVRLFIQVPLWQDNALAMLLALVGGASILLGNLLAISQTSLKRLL
                     AYSSIAHFGYLMIALTIVAQHGSRSVWVYLATYVIATLSAFAVLSSLTGIKDDTQGDR
                     FEALRGLYQRNPLLALALALSLISMAGIPLTAGFVGKFALFASGVASGQWVLLLLVVA
                     GSAIGIYYYLRVVSVLFQRSGQTLTAGVNVPTLNGILIAALSFMTLAIGIWPDFLI"
BASE COUNT          426 a          646 c          682 g          368 t
ORIGIN      
        1 catgccaccg tagaacagca ggtcggcgtt caggcccttg atcttggtga ggatggcggt
       61 gaaatcggtg gacttgtcgg tggtgaattc gtggccgacg atggtggcgc cgccggcaac
      121 ggcagctttt tcgaactcgt ctgccagacc ctggccatag gccgtgcggt cgtcgatgat
      181 ggcgatggtt ttcgctttca tctcaccgac ggcaaacttg cccagcacgc tgccctgctg
      241 cgcatcattc gccatcacgc ggaacgcgtt cttgaaaccc tgctgggtgt atctgacgtt
      301 ggtggcggag ggggaaatct gcgggatctt ggcgtcggcg taaatgcgcg aggccggaat
      361 cgtggtgccg gaattcaggt ggccaatgat cgcatgcact ttttcgtcga cgaatttctg
      421 cgctacggtg gtggcaatgc gcggatcggc ctgatcgtct tccgacagca gttcgaattt
      481 caccggcttg ccgccaatca ccggtgggtt ggcattcagt tcgtcgatcg ccagccgcac
      541 gccgttttcg ttgtccttgc ccagatgcgc ctgcgggccg gtcatgggcg ctgcgtggcc
      601 cactttcacg atcagttcgg cgctggcagc ggttttggag ccttcgtcgc tgcaggccgc
      661 caggcccagt accgccatca gaccgaacac atgtcgcacg tatttcataa tgacgaaatc
      721 cttcgagttt agggttgggc cgccgctacc ggcgctgttg tttttccggt tggttcgatg
      781 gggaatctgc aaccggctct ggatcgcaaa gcaagcagga aatgcgccat tctataaagc
      841 tgtggggggc aggtcgagag gtactggcgg gaaacggtgc aacggaaaac gacatcacgg
      901 gaaacggcag cgcccgctca ggaatccctg ggcaggcgcc gggccgcgtc agatcagaaa
      961 atccggccag atgccgatgg ccagggtcat gaaggacagg gctgcgatca ggatgccgtt
     1021 gagggtgggc acgttcacgc cggcagtcag ggtttggcca ctgcgctgga acagcaccga
     1081 caccacccgc aggtagtagt aaatgccgat ggcgctgcct gccaccacca gcaacagcag
     1141 cacccattgt ccgctggcca caccgctggc gaacagggcg aacttgccga cgaaaccggc
     1201 ggtcagcgga atgccggcca tcgagatcag gctcagcgcc agcgccaggg ccagcagcgg
     1261 attgcgctgg tacaggccgc gcagggcctc gaagcggtca ccctgggtgt cgtccttgat
     1321 gccggtgagc gaactcagta ccgcaaaggc gctcagcgtg gcaatcacgt acgtggccag
     1381 gtagacccat accgagcggg agccatgctg ggccacgatg gtcagcgcaa tcatcaggta
     1441 accgaaatgg gcgatggagg aataggccag caggcgcttg agcgaggtct ggctgatggc
     1501 cagcaggttg cccagcagga tcgaagcgcc acccaccagg gccagcagca tggccagcgc
     1561 attgtcctgc cacaggggca cctggatgaa caggcgcacc agcactgcca ccacggccac
     1621 cttgctggcg gtggccagaa aggcggtcac cggcatgggc gcgccctggt acacgtccgg
     1681 ggtccaggtg tggaacggca cccacgacag cttgaagccc agcccgatca ggatcatggc
     1741 gcagcccagc agacccggcg atgaatccac cggcaggctg gcccattgcg ccagcagcag
     1801 gctgccggtt tccgcataaa tcagggcaat gccgaacagc aggaacgccg acgcggtggc
     1861 cgacagcacc agatacttga taccggcttc cagcgagcgt ttgtttttca gcgcgaacgc
     1921 cgccagtccg tacagcggca ccgacatcag ttccagcccg atgaacaggg ctgccatgtg
     1981 gctgctggcc gccagcacca tgccaccgca cgccgacacc agcagcaaca ggtaccattc
     2041 ctcgcgaaaa ctggcggcgc tgcgcacata cgcatgcgac acaccgatgc acaccagggt
     2101 gctgagcagg atcatgctcc ag
//
LOCUS       DASUEG010000759         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_958868, whole genome shotgun sequence.
ACCESSION   DASUEG010000759 DASUEG010000000
VERSION     DASUEG010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958868"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFX11_14335"
     CDS             complement(<1..258)
                     /locus_tag="VFX11_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4939852.1"
                     /translation="MSETDSKVESQSSFLEDLKETLRRGKTIDGYDYNVKKRYYEPAP
                     ESSYGDEFRQVVESRRSVRKFTDRKIPRDVLDACLDLALLAP"
     gene            complement(301..1254)
                     /locus_tag="VFX11_14340"
     CDS             complement(301..1254)
                     /locus_tag="VFX11_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939853.1"
                     /translation="MIYDFDLATKPTAEELATCLDPRSFKMSTARIYAEPRLPSPWLA
                     CDGPLSGRSHDEIYQCADQAFWEGFSDGRLDKRVSAEKRVGEALALLQDWDEQEKLAR
                     IHFLRGSLRMAMALENGKLQYAMFNNYYVGRDMRRSEELDPDGIAAPAFLNALDMANA
                     AVFKRWNAATTLADAAIVDAYHVGIGAAFAVGGTLIGFPLATGIPDKTVTMMEEVPCD
                     IYFCTDNTQKAPFAVPGLAFHQAEGYARVGDRENMMRWIETARTAPGYEDWFFNSIVE
                     GVAADPDAFLQKFSQYGNDGAPFMEVYANSNFGCVFCHGRM"
     gene            complement(1502..1903)
                     /locus_tag="VFX11_14345"
     CDS             complement(1502..1903)
                     /locus_tag="VFX11_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4939854.1"
                     /translation="MSQTSPSQDAIITVRPDHTVDTLQKLPNYAGISGVTAGATGISM
                     NRVIIPPGARAEPHFHRGFETAIYLLQGRVETRYGHNLQHSVINVAGDFIFIPAGVPH
                     QPINLSTSETAIAIVARNDPNEQENVQAYQP"
     gene            2173..>4002
                     /locus_tag="VFX11_14350"
     CDS             2173..>4002
                     /locus_tag="VFX11_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4939855.1"
                     /translation="MPLSSHTVHVPPSRLALFLLFTLLLALPQAHALAVSLSAPEQVN
                     ADSPLNIGLEGVNNPRDFITIVKPDESEGRYNDYEYTKGDSVQLFAPVEPGDYEIRWL
                     GAESPYPTLARQKLNVLPVSAQLQVPATAAIGTAISIQWQGPDNPRDFITLVPAGTRE
                     GQYREYVYTSEGKSVSLTAPVEPGNYEIRYLTGRGNKSLGSAPIQITDSAASVQFASP
                     ARIGQTLSIQWTGPANPREFITVVKAGSRDGHYAGYTYVDKGNPLDLLLPEVPGNYEI
                     RYMTADSDRVLARAPLKIETVAASVDGPATAVARSLIQVKWSGPDNAQDYIAITPAGK
                     PASYIHYSYTARGNPVTSEVPKDPGDYDIHYLTANSAQSLAMAKLKVTPSATPGKLQV
                     MPPTDTAKNATFQTVEVVLDASGSMLQKLDGKRRIDIAKTTLTQLPGMLEAGKQQFAL
                     RVFGHRKADSCDTELLLPPAPLDKAAISRTIGKLEAKNLARTPIADSLKLVQQDLANS
                     PGKALVILLTDGEETCDGDPAAAIKQLRASGVDVRVNVIGFAIGEYALKQQFSGWAEI
                     GGGTYFDAQDAAALQKALTQSLQLSFMVLDEKGVAIASGNAGDA"
BASE COUNT          875 a         1197 c         1152 g          778 t
ORIGIN      
        1 gggggccagc aacgccagat ccaggcaggc atccagcaca tccctgggga tcttgcggtc
       61 ggtaaacttg cgcaccgaac gccggctttc cactacctgc cggaactcat caccgtagct
      121 gctttccggc gccggctcgt aatagcgctt ctttacgttg taatcgtagc cgtcgatggt
      181 cttgcctctg cgcaaggttt ctttcaggtc ttccagaaac gagctctggg attccacttt
      241 gctgtcggtc tcagacatga ttcattaact ccgctggttc aggtgattgc aaaaaaggtg
      301 ctacatccgc ccatggcaga acacgcaacc aaaattgctg ttcgcgtaca cttccataaa
      361 gggcgcgcca tcgtttccgt actgcgagaa tttctgcaga aaggcatccg gatctgccgc
      421 cacgccttcc acgatagagt tgaagaacca gtcttcatag cccggcgccg tgcgcgcggt
      481 ttcgatccag cgcatcatgt tctccctgtc tccaacgcgg gcatagcctt ctgcctggtg
      541 aaacgccagg cccggaacgg cgaacggtgc cttctgggta ttgtcggtgc aaaaatagat
      601 atcgcagggc acctcttcca tcatggtcac cgttttgtcc ggtatgccgg tcgccaatgg
      661 aaatccgatc agcgtgccgc cgaccgcgaa ggccgcaccg atgcccacat ggtaggcatc
      721 gacaatggcg gcatccgcca gcgtggtggc cgcgttccag cgtttgaaaa cagcggcatt
      781 ggccatgtcg agtgcgttga gaaacgcagg cgcggcgatg ccatcggggt ccagctcttc
      841 cgaccgtcgc atgtctctgc cgacatagta attgttgaac atggcgtact gcagcttgcc
      901 gttttcaagc gccatggcca tgcgcagcga tccacgcaga aagtgtatgc gcgcgagctt
      961 ttcctgctca tcccagtcct gcaacaatgc caaagcctcg cccacgcgtt tttccgcgct
     1021 cacccgcttg tcgaggcggc cgtccgagaa tccctcccag aaggcctgat ccgcgcattg
     1081 atagatctcg tcgtggctgc ggccggacaa cggaccgtca caggccagcc agggactggg
     1141 caggcgcggc tctgcgtaga tacgcgcggt ggacatcttg aaggaacggg ggtccagaca
     1201 ggttgccagc tcttcagccg tgggcttggt ggcaagatca aaatcgtaga tcacgggaac
     1261 cttgcacagg ggtactttcg aacatcccgt caacatcatc gccgccaagg caagcgccgc
     1321 ccaatatgcc ctcatcaaaa caccccgtct tgttgttatg ccgtttggtc aattttcaga
     1381 atccagctgg acattttccc accaaacaac ccccgttaat caagtccctt gcgcaaattc
     1441 gtgtcctcat gcgccagtcg gcgcgcgtcc gtcaccgtga atgacctaac cggaatagat
     1501 gctagggctg gtaggcctgc acgttttcct gctcgttggg atcattgcgc gccacgatgg
     1561 caatggcggt ttcgctggtg ctcaggttga tgggctggtg gggcacgccg gcaggaatga
     1621 agatgaaatc gccggccacg ttgataacgg aatgctgcag attgtggccg tagcgggttt
     1681 ccactcgccc ctgcagcagg tagatcgccg tttcaaaacc acgatgaaaa tgcggttcgg
     1741 cgcgggcacc ggggggaatg atcacccggt tcatggagat gcccgtggcg cccgcggtga
     1801 cgccggaaat accagcataa ttcggcagtt tctgcagggt gtcgacggtg tgatcggggc
     1861 gaacggtgat gatggcgtcc tggctggggc tggtctggga cataacggga attccggaaa
     1921 aggtggaagc gacacccgat tctacgccag cacgccggta gaacgacagc gaggccatac
     1981 cgcgttgaat cacgaaatcc ggccgcgcct gacatcatca atttgcgtca tttttcacaa
     2041 aggggccgct gcagtggcag gctgaattgt cagtgcaaag gcggacatgc cagaatccgc
     2101 gccggtacgg ggttgattcg ataaatgatt caacccaacc gcttcgttca cctcgttgca
     2161 cagggaaatc acatgccgct gtccagccac accgttcatg ttccgccatc acgacttgca
     2221 ctgtttttgc tgttcaccct gctgctggca ttgccgcagg cacatgcatt ggcggtgtcg
     2281 ctgtcagctc cggagcaggt caatgccgac agcccgctga acattggcct ggaaggtgtc
     2341 aacaatccgc gggatttcat caccatcgtc aaacccgacg agtccgaagg ccggtacaac
     2401 gactatgaat acaccaaggg tgacagcgtg caactgttcg cgccggtgga accgggcgat
     2461 tatgaaatcc gctggctggg tgccgagagc ccctatccca ccctggcccg gcaaaaactg
     2521 aacgtgctgc cggtgtcggc gcaactgcag gtgcccgcca cggcagccat cggtacggcc
     2581 atttccatcc agtggcaagg gccggacaat ccacgggatt tcattaccct cgtgcccgcc
     2641 ggtacaaggg aaggccagta cagggaatac gtttatacca gcgagggaaa atccgtttca
     2701 ctgacggcac cggtcgagcc aggaaattat gaaatccgtt acctcaccgg acgtggcaac
     2761 aagtcgctcg gcagcgcacc gatccagatc accgactctg ccgccagcgt gcagttcgcc
     2821 tcgcccgcac gcatcggaca aacgctgtcg atccagtgga caggccccgc caatccgcgc
     2881 gaattcatca ccgtggtcaa ggctggttcc cgtgacggtc actatgctgg ctatacctat
     2941 gtcgataaag gcaatccgct ggacctgctg ttgcctgaag tacccggcaa ttacgaaatc
     3001 cgttacatga cggccgattc cgatcgcgtg ctggcgcggg cgccgctgaa aatcgaaacg
     3061 gtggcggcca gcgtggacgg gccggcaacg gccgtggcgc gcagcctgat tcaggtaaaa
     3121 tggagcggcc ccgacaatgc acaggattac attgccatca caccggcggg caaacccgct
     3181 tcgtatattc actacagcta caccgcgcgc ggcaatccgg taaccagcga agtaccgaaa
     3241 gatccggggg actatgacat ccactacctc accgccaaca gcgcgcagtc gctggccatg
     3301 gcaaaactga aggtaacccc ctccgcgaca ccaggcaaac tgcaggtgat gccaccgaca
     3361 gacaccgcaa aaaatgcaac attccagacc gtggaagtgg tgctggatgc atccggcagc
     3421 atgctgcaaa aactggatgg caagcgccgg atcgatatag ccaaaaccac gctcactcaa
     3481 ctaccgggca tgctggaagc gggcaagcag cagtttgcgc tgcgggtgtt cggccatcgc
     3541 aaggccgaca gttgcgacac tgaactgctg ttgccgcccg ctccgctgga caaggccgcc
     3601 atcagccgga ccatcggcaa actggaagca aaaaatctgg cgcgcacccc cattgccgat
     3661 tcgctgaagc tggtgcaaca ggatctggcg aacagccccg gaaaggcgct ggtgatcctg
     3721 ctaactgacg gtgaagaaac ctgtgacggc gatccggcgg cagcaatcaa acaactgcgg
     3781 gcgtcgggcg tcgatgtgcg ggtgaatgtc atcggtttcg ccatcggcga atatgcgctg
     3841 aagcaacagt tttccggctg ggctgaaatc ggcggcggca cctatttcga tgcgcaggat
     3901 gcggcagccc tgcagaaggc gttgacgcaa tcgctgcaat tatccttcat ggtgctggac
     3961 gaaaaaggtg tggcaatcgc atcgggcaat gcaggcgatg cg
//
LOCUS       DASUEG010000760         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_517501, whole genome shotgun sequence.
ACCESSION   DASUEG010000760 DASUEG010000000
VERSION     DASUEG010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517501"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFX11_14355"
     CDS             complement(<1..723)
                     /locus_tag="VFX11_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4939856.1"
                     /translation="MKTITNHRTISTLLALWVLLPGFLLLSACGGDKGTINTSWTTPE
                     SLIFAYPYPGQQEVPPSTQMVLRFSAPLNETVIEEIDDRLQLVTVAGGNPAMFVHETV
                     DNGRGLLLRPVDELVPNTEYQLQLSEVGLDGIDTGPFNDFRFRTAGAQKGFAGAIGDG
                     DFKLLAATPLDQPALLTDIDDARQPNDMSTLRMAFNRVLDPASVNYGNQVRLENSQGS
                     LIEATLLLQGRYLVLDPRDDLEP"
     gene            complement(771..2237)
                     /locus_tag="VFX11_14360"
     CDS             complement(771..2237)
                     /locus_tag="VFX11_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4939857.1"
                     /translation="MANQVKDSFKQPKTTTRKITQKKGGNCMACTIHRLLPTTYSLFL
                     TAAIIGTQPDDAFAGAGDGASTYGLGPVNAGSAQAFSAFDPGAWAVYYNPAALARTPE
                     GELSIVLQYGDQELRAKSLGGSDPVTRENDVLSDTSSELALLGIKARIGGASDTTRPM
                     YLGINVGVDEYISNILPYQANTAEQGQFLRYESQPLYLAFGGAVRDLVRGVDVGFSAR
                     LTLAAKARLVAVSDLAGNTDSEQISMKAEPSLSPAVGLNVRTSELFCGHSPWRPSSCL
                     PFGLDGLEVALFWRDETNYEVSVDANVVIPGVIPEPGLDLALSTIDSYQPPVFGGSFL
                     LPLGKLELVASVEQQQWSELESQFSGDTIRDQADLRFDDVVVSRAGASFRWSDQLRLF
                     GGVALEDSPLKSSRSQDVNFLDADKMVLGVGGDYRLNSAPIINAPLVLSLAYQYQKLD
                     ERDFELTSINSSSDPAPYETVRADGEIHVFSLSASLKF"
     gene            complement(2726..3949)
                     /locus_tag="VFX11_14365"
     CDS             complement(2726..3949)
                     /locus_tag="VFX11_14365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939858.1"
                     /translation="MKKRSRGGTQQQTAENTPLIGMLWLRNPLDWHPIDRFILLASLV
                     MLATVVFSIALAGTIALAPQYLALPVAWTLLGLYGLHIILLASYIWLAWKRRRRENDW
                     PAFENIVIAGFVINIMLSSYLTGTYFTHGLLFFFVGVNIASALTWVTKILVAYWTVLV
                     TLITFATIDFSGAFKHAPLLSRSVYQPDGSPVIGWLFIEVTVAAIHYSVLYLCMLVIR
                     RWAERENLYREMSNVDGLTRLINRRCFIERAKNELARAKRLSSNTPKSLGCIMLDLDH
                     FKHINDTWGHHAGDAVLVAASEIMMANARPYDEVGRYGGEEFAILLPGVTLKIAAGIA
                     ERLRKAISDAVVEIDGNRISISASFGVACYPTHAISDLNDLLKAADQALYEAKRGGRN
                     RVILANTTDQDGVTG"
BASE COUNT          998 a         1149 c         1290 g          981 t
ORIGIN      
        1 cggttcgagg tcatcccttg ggtcgagcac aagatagcgg ccctgcagta gcagagtggc
       61 ctcgatcagg ctgccctgcg aattttcaag acgaacctga tttccatagt tgacgctagc
      121 cggatcaagt acgcgattga acgccattct cagggtgctc atgtcattcg gctgcctcgc
      181 atcgtcaatg tcggtaagca gcgcgggctg atccaggggt gtagcggcga gcagtttgaa
      241 gtcgccgtcg ccaattgccc ccgcgaagcc tttctgcgca ccggcagtgc gaaagcgaaa
      301 gtcgttgaag gggcctgtgt caatgccgtc taggcccact tcgctcagct gcagttggta
      361 ttcagtattc ggcacgagtt cgtcgacagg acgtagcagc aggccacgtc cgttatccac
      421 cgtttcgtgg acgaacatgg cagggttgcc gcccgctaca gtcaccaatt gcaatctgtc
      481 gtcgatttcc tcaatgacgg tttcattgag gggcgccgaa aaacgaagca ccatctgggt
      541 gctgggtggc acttcctgct gtcccgggta ggggtaggcg aaaataagac tctccggcgt
      601 tgtccaggaa gtgttgatgg tgcctttgtc gccgccacat gcggacagca agaggaaacc
      661 aggcagcagt acccagagtg caagaagcgt ggatattgtt cggtgatttg ttattgtttt
      721 catgatggtt ttctccttat tgttattagt cgcctccgtc gaagctccgc ttagaacttc
      781 agggaagcgg ataaagagaa gacgtgaatc tctccgtcgg cgcgtactgt ttcgtaaggt
      841 gccggatcgg aagacgagtt gatggacgtc agctcgaaat cgcgctcgtc gagcttctgg
      901 tactgatagg ccagcgacag caccagggga gcattaatga tgggcgcact atttaggcga
      961 tagtcgccac caacgcccag caccattttg tcggcatcca ggaagttgac gtcttgtgag
     1021 cgggaagatt tcaatggcga atcctccagc gccaccccgc caaacagcct gagctggtca
     1081 gaccaccgga agctggcgcc agcgcgggaa acgacgacgt catcgaaacg caagtccgcc
     1141 tggtccctaa tggtgtcccc ggaaaactga ctttccagct cggaccactg ctgttgttcg
     1201 acagaggcca caagctcgag cttcccaaga ggcagcagga agctgccgcc gaacactggc
     1261 ggctggtacg agtcaatggt agaaagcgcc agatcaagtc ccggttcggg gatgacgccg
     1321 gggataacca cgttggcatc cacactgacc tcgtagttgg tttcgtcgcg ccagaatagt
     1381 gccacctcca gtccatcgag tccgaacggc agacaagatg aaggacgcca aggtgaatgg
     1441 ccacagaata gctcggaagt gcggacgtta aggcccacag cgggggacag ggaaggctct
     1501 gccttcatcg aaatctgctc agaatcggta ttgcctgcga gatcggagac cgctacaaga
     1561 cgagcctttg ccgccagtgt caagcgtgct gaaaagccga catcgacccc tcgaacgaga
     1621 tcacgaacag cgccaccaaa tgccagataa agcggctggg actcgtagcg caggaattgg
     1681 ccttgctctg cggtattggc ctggtacggt agaatgttgg agatgtattc atcgacgcca
     1741 acgtttattc caaggtacat cgggcgggtt gtgtccgatg cgccgccaat acgggctttg
     1801 atgcccagca atgcaagctc cgaactggta tccgatagta cgtcattctc gcgtgtcacc
     1861 gggtcagacc ctcccagcga ttttgctcgc agttcctggt cgccgtattg cagcacaata
     1921 ctcagctcac cttccggggt gcgggctagg gcggcggggt tatagtacac cgcccaggca
     1981 ccgggatcga aggcgctgaa agcctgggcg gaaccggcgt tgaccgggcc aaggccatag
     2041 gtggatgctc cgtcgcctgc gccggcaaag gcatcgtcgg gctgtgtgcc aattattgct
     2101 gctgtcagga acaggctgta ggtggtcggc aacaaccggt gaattgtgca tgccatgcaa
     2161 ttaccccctt ttttttgtgt tatttttctt gtggttgttt ttggttgttt gaacgagtct
     2221 tttacttggt tggccaacca acgtcaagag aaagatgcac aaacgaatga tgaccaggaa
     2281 tacgtgaaac gtaatattgg gcaatagggc attcgggata tttgtcgcgt taggagatca
     2341 ggggtggtgc tcgggcagaa cggagagtag cggagcaaga ggcgcgggaa cggtccttga
     2401 atgtgggagc tggcagaggg catttctcgt gaagcgactt gccctcgcgc ttccattcgc
     2461 tcgaaatttt tcttgtaacg aacactgcat tgcgggccgt tttgcaaacg gcatcggcgg
     2521 atagccctct tacttcgcca aaggctcaga gatatcgccg cagccgttgt attgatactt
     2581 agccatgtcg aaactccccg gcaccgtaga gggcgacgaa cctgggggcg ttttgccggt
     2641 tgaaacctta ccccggtcgc cccccgacta tgctgcaggg ccagcactga ctttcatgaa
     2701 ggtagctgcg ccgaaacaca gtgagttatc cggtgacacc gtcctgatcg gtggtattcg
     2761 ccaaaattac gcgattgcgg ccgcctcgtt tggcctcgta gagggcctga tcggccgctt
     2821 tgagcaagtc gttcaggtcg gagatggcgt gggtcgggta gcaggcgacg ccgaagcttg
     2881 cggaaatgct gatacgattg ccatcgattt ccaccactgc gtcgctgatt gccttgcgca
     2941 gtcgctcggc gattccggcc gcgattttca gtgtcacgcc cgggagcaga atagcgaact
     3001 cctccccgcc atagcgaccc acctcatcgt acgggcgtgc gttggccatc atgatctccg
     3061 acgctgctac caataccgca tcgccggcgt ggtgacccca agtgtcgttg atgtgcttga
     3121 agtggtctag gtccagcatt atgcagccca gggacttcgg cgtgttactg gacaatctct
     3181 tggctcgtgc cagttcattt tttgcccgtt cgatgaagca gcggcggttg atcagacggg
     3241 tgaggccatc gacgttggac atttcacgat agaggttttc cctttctgcc cagcgtcgga
     3301 tgacaagcat gcagaggtat aggacggagt aatgaatagc ggcgactgtg acttctatga
     3361 acaaccaccc gatcaccggt gagccgtcag gctgatatac ggaccgggac agcaagggtg
     3421 catgtttaaa cgcgccgcta aagtcaatgg tggcgaatgt gattagggtc accaagacgg
     3481 tccagtaggc gacgagtatc tttgttaccc aggtcaacgc cgaagcgatg ttgacaccaa
     3541 cgaaaaaaaa cagaagccca tgggtgaaat acgtgccggt aagatagctg ctcagcatga
     3601 tattgatcac gaaaccggcg atgacaatgt tttcaaatgc gggccagtcg ttttccctgc
     3661 gacgtcgttt ccatgccagc cagatgtagc tggcgagtaa gatgatatgc aggccgtaca
     3721 gaccgagcaa cgtccaggcg acaggcagtg caaggtattg cggcgccagc gctatggtgc
     3781 cagcaagcgc aatgctaaac acaacagtcg ccagcataac caggcttgca agcagaataa
     3841 aacggtcaat gggatgccag tccaaggggt tcctgagcca aagcatacca ataagaggcg
     3901 tgttttctgc agtttgctgc tgtgtgccgc ctcgagagcg ctttttcatt tttattcccc
     3961 ccgggggctg aagagacaaa tgccaacaag ccagattagg aaagtgtaga cgaatactgc
     4021 ttcctgcctc gcttgttgaa ctggtgtgat tttgcagcga gtatcgatta tgtagttggc
     4081 atggctagca accgcaaatt gatggcaaaa gcaggcatct gtccggattt acctatgtcc
     4141 tgatgcaggg attgcgcagt ctggcactgc aacatactgc gctggaaaaa tgccagatga
     4201 atacactgcg gttgaagcta ttgtgcgcta gcgatggata gacaggacca cgacctgatg
     4261 ttttgctatt tcgtcgggct ggatatggat gatggagtct agaactattc tatgttagcc
     4321 aagaacagag atcgcttgtt gtcaggcaaa ttttcagacc atcctcaagc cctgggcggc
     4381 atagcccctc gcgaactgac acgctgcctg tactgctc
//
LOCUS       DASUEG010000761         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1893605, whole genome shotgun sequence.
ACCESSION   DASUEG010000761 DASUEG010000000
VERSION     DASUEG010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893605"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFX11_14370"
     CDS             <1..321
                     /locus_tag="VFX11_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939859.1"
                     /translation="LEGKNILLNMEWFTEPLHGGETSYAFATALDQRYGVIPVLSQYS
                     RENRYPIGQFVQTSGVKRGSYIWGKPTRVVSYSDDPDFEAAVNSFTYQMSRYAAFYTG
                     FTSY"
     gene            complement(349..924)
                     /locus_tag="VFX11_14375"
     CDS             complement(349..924)
                     /locus_tag="VFX11_14375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4939860.1"
                     /translation="MRSGILIVLMCGLAGCATQSSTVLEPVEQQPGWPAPAEGGRYPA
                     QSPTPGDSGTGYSAPRTGSTSSTARRPATVAYSPNSTGVTAADALLREGNALHQRGEY
                     SGAANNFERGIRMAPRSPALYLALAKTRLAMGDNSNAIQMANRALSLLPSGSWAADDA
                     RTEAWTVIANAREAQGDRTGAAAARQKAKGW"
     gene            complement(939..3203)
                     /gene="mrcB"
                     /locus_tag="VFX11_14380"
     CDS             complement(939..3203)
                     /gene="mrcB"
                     /locus_tag="VFX11_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEX4939861.1"
                     /translation="MRRWGGLLLKLGLIGLVLGAVGVAYLDAKITQHFEGKRWAIPAK
                     VFARPLEIYDGLKLPEAQLSWELSLLNYRLVSAKQPETGAYTRNGAVFQIGTRGFDTW
                     EGAEKPHQVRFVLDHGRVESLTSDGKTGAIVRLEPALVGGIYPAHNEDRVLVKLDQVP
                     KLVTDALIAVEDRDFYTHFGLSPRGIGRALFTNVTSGSVRQGGSTLTQQLVKNFYLSS
                     ERSLVRKGTEAVMAVLLDAHYSKDEILEAYLNEVYLGQAGKRGIYGFGSASLFYFGQP
                     LRELKPEQVALLIGLVKGPSWYDPRRHPERSLDRRNQVLKTMQEQGVLNDAEYDFAVK
                     KPLGVIKKPLYDDARYPAFLDLIKRQLKQDYQDEDMRSEGLRVFTTLDPWAQEQLEKA
                     VEQQTAQLEKSFGKRLENLEAAGVITASNTGEVVAMVGGRSFRYEGFNRALDAMRPIG
                     SLAKPPVYLAALEQGFTLASILQDEPLQHKLADGKIWSPENYDKQSHGEVPLVNALAN
                     SYNQATVSLALQVGPTKVADVMKRLGAPRPIDPVPALALGSVDLSPFEVAQMYQTIAS
                     EGFNTHLRSVRSVLNADGKPLQRYDLEVQQRFAPADIYVLTHAMQEVIRSGTARSTNS
                     FITPDYHLAGKTGTSNDQRDSWFAGFSGNYLGVVWMGRDDNEKTPLTGASGALRVWNQ
                     TFSKLALQPLELNQPADVEWVWVDPLQQVQTDAECPGSIEVPMRIESIPEQTIPCNPG
                     GRVFNWFRGLVNPE"
BASE COUNT          577 a         1054 c          985 g          661 t
ORIGIN      
        1 ctggaaggga aaaacattct cctgaacatg gagtggttca ccgaacccct gcacggtggc
       61 gagacgtctt acgcgttcgc caccgctctt gaccaacgtt acggggttat tcctgtgctg
      121 tcgcaataca gtcgcgagaa ccggtatccg atcggacaat tcgtgcagac aagcggcgtc
      181 aaaaggggca gttatatctg gggcaagccc acacgggtgg tgagctactc cgacgatccc
      241 gacttcgaag ctgctgtcaa ttcattcacc tatcaaatga gcaggtacgc ggcattctat
      301 acgggcttca ccagctactg atcgaaccga cggccggcag cctgcgcatt accatccctt
      361 cgccttctgc cgcgccgcag ccgcccctgt tctgtcaccc tgcgcttccc gtgcattcgc
      421 aatgacagtc caggcttcgg tacgggcatc gtcggcggcc caactgccgc tgggcagcag
      481 tgaaagggca cggttggcca tctggatcgc gttgctgttg tcgcccatgg ccaggcgggt
      541 ttttgccagc gccagataca acgccggcga acgcggtgcc atgcggattc cgcgttcaaa
      601 attattggcg gcaccgctgt attcaccgcg ctggtgcagc gcattgcctt cacgcaacaa
      661 tgcatcggcg gcggttacgc cggtactgtt gggcgagtag gccaccgtgg cggggcgacg
      721 tgcagtggat gaggtcgagc ctgtgcgggg cgcggaataa cccgtgccgc tgtcaccggg
      781 cgttggcgac tgcgccgggt aacgtccgcc ttccgccggg gctggccagc caggctgctg
      841 ttccaccggc tccagcacgg tagacgactg cgtggcgcag cccgccagtc cgcacatcaa
      901 cacaatcagg attccactac gcatacttgc tcccatcatt attcaggatt caccagacca
      961 cgaaaccagt tgaacacgcg cccgccagga ttgcagggga tcgtctgctc cggaatcgac
     1021 tcgatccgca tcggcacttc gatcgagccc gggcattccg catcggtctg cacctgctgc
     1081 agcgggtcga cccacaccca ctccacatcc gccggctggt tcagttccag cggctgcagc
     1141 gccagtttcg aaaacgtctg attccacacc cgcagcgcgc cgctggcacc ggtgagcggg
     1201 gttttttcgt tgtcgtcgcg gcccatccac accacgccca gataattgcc ggagaaaccg
     1261 gcgaaccagc tgtcacgctg atcgttgctg gtgccggtct tgcccgccag atggtagtcc
     1321 ggcgtgatga aggaattcgt gctgcgggcg gtgccgctgc gaatcacttc ctgcatggca
     1381 tgggtgagta cgtaaatatc cgccggcgcg aagcgttgct gcacttccag atcatagcgc
     1441 tgcagcggct tgccgtcggc attgagcacc gagcgcacgc tgcgcaggtg ggtgttgaat
     1501 ccttcggatg caatcgtctg gtacatctgc gcgacttcaa acggcgacag gtccaccgag
     1561 cccagcgcca gtgcgggtac tggatcgatg gggcggggcg cgccgagacg tttcatcaca
     1621 tctgccacct tcgttggccc tacttgcagc gccaggctca cggtggcctg gttgtaggaa
     1681 ttggccaggg cgttcaccag tggcacttcg ccgtggctct gcttgtcgta attctccgga
     1741 ctccagatct tgccatctgc cagcttatgt tgcagtggct cgtcctgcag gatcgacgcg
     1801 agggtaaagc cctgctccag cgccgccagg tacaccggcg gttttgccag cgagccgatg
     1861 gggcgcatgg catccagcgc gcggttgaaa ccttcgtagc ggaaggaacg cccgcccacc
     1921 atcgcgacca cctcgcccgt attgctggcg gtaatcacgc cggctgcttc tagattctcc
     1981 agccgcttgc cgaaagattt ctccagttgc gcggtctgct gttccaccgc tttctccagc
     2041 tgttcctgcg cccagggatc gagcgtggtg aacacccgca ggccttcgga acgcatgtct
     2101 tcgtcctggt agtcctgttt cagctgacgc ttgatcagat cgaggaaagc cgggtagcgg
     2161 gcgtcgtcgt acaggggttt cttgatcacg cccaagggct ttttcacggc gaaatcatat
     2221 tcggcgtcgt tgagcacgcc ctgctcctgc atggtcttga gcacctggtt gcggcgatca
     2281 agggaacgct ccggatgacg gcggggatcg taccagctgg ggcctttcac caggccgatc
     2341 agcagcgcca cctgctcggg cttcaattcc cgcaggggct ggccgaagta gaacaggctg
     2401 gcactgccga agccgtaaat gccgcgcttg cccgcctgtc ccagatagac ttcgttcaga
     2461 taggcttcga ggatttcgtc cttggaataa tgggcgtcca gcagcacggc catgacggcc
     2521 tcggtgccct tgcgcaccag tgagcgttcg ctgctgagat agaaattctt caccagctgc
     2581 tgggtgaggg tactcccccc ctgccgcacc gagccggacg tgacgttggt gaacagggcg
     2641 cggccaatgc cccggggcga caggccaaaa tgggtgtaaa aatcccggtc ctccactgcg
     2701 atcagggcgt cggtcaccag tttgggcacc tggtccagct tcaccagcac ccggtcttcg
     2761 ttatgggcgg gataaatgcc gcccaccagc gccggttcca gccgcacgat ggcgccggtc
     2821 ttgccgtcac tggtcaggct ttccacccgt ccgtgatcca gcacgaagcg cacctgatgg
     2881 ggcttttccg cgccctccca ggtatcgaag ccccgggtgc cgatctggaa taccgcgcca
     2941 ttgcgggtgt aggcgccggt ctccggctgt tttgcgctga ccaggcggta gttgagcagg
     3001 ctcagctccc agctcagttg cgcctcgggc agtttcaggc cgtcatagat ttccagcggg
     3061 cgggcgaaga ctttcgccgg aatcgcccag cgtttgccct cgaaatgctg ggtaatcttg
     3121 gcatccagat aagccacacc caccgcgccc agcaccagac cgatcagtcc cagctttaac
     3181 agcaggccgc cccagcggcg caggaacgac ctgccccgcc ccttgccctt ggcagaggcc
     3241 ttgcggaccg acttttttgc gccggcggct ttcggct
//
LOCUS       DASUEG010000762         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1454617, whole genome shotgun sequence.
ACCESSION   DASUEG010000762 DASUEG010000000
VERSION     DASUEG010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454617"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFX11_14385"
     CDS             complement(<1..572)
                     /locus_tag="VFX11_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939862.1"
                     /translation="MKTVLVTGAGRGLGLEFAKQYLEQGNRVIATARDLAKVTALQAL
                     AKQYPELLTLHTLNVTDAHSRQQLARDLDGQPIHLLINNAGVSGGWGSLGKLDEAAWL
                     QVLHTNAIAPIKMVEALRANLANAEVSTVAILSSKMGSMGDNTSGGSYIYRSSKAALN
                     AAAKSLAIDLAGDNIKVAILHPGWVRTDMGG"
     gene            complement(635..1150)
                     /gene="greB"
                     /locus_tag="VFX11_14390"
     CDS             complement(635..1150)
                     /gene="greB"
                     /locus_tag="VFX11_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX4939863.1"
                     /translation="MSRYRPPSKPSAPYITPEGRDRLMAELRDKSEKLRPEVVKALSA
                     AAAEGDRSENAEYIYRKKQLREIDSRIRYLVKRLEAVKAVEQKPADQSRVFFGAWVEL
                     EEVESGTLERYRLVGADEIDLDNGWISVDSPMARALLKKQEGDEVTVQRPKGVAAFEI
                     VRVQYEPFATD"
     gene            complement(1160..1603)
                     /locus_tag="VFX11_14395"
     CDS             complement(1160..1603)
                     /locus_tag="VFX11_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939864.1"
                     /translation="MHYDAQIQMIVSTLKLAQTLAVDPETGKSGMAWNAKLEGGCKSS
                     HLAEKQAVVQIREANRLAGPHIGQVRQRDGTLQITVVAPRDYFTGLIALFPHCIPEQG
                     GYLSFNFLLGSEVEMSQQGPVMKVLSVEYKMVKELSSNPDLISRN"
     gene            1713..2291
                     /locus_tag="VFX11_14400"
     CDS             1713..2291
                     /locus_tag="VFX11_14400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX4939865.1"
                     /translation="MTTTPAILVFAGSARRESLNKKLARAAADSARSTGAQVTLLDLR
                     DYPIPLYDGDLEAESGVPENARALRRLMATHHGLIVVSPEYNGFITPLLKNTLDWISR
                     PDGAEDGLTLYRDKVALVLSASPGALGGLRSLQLIRQLLGNLGVTVLPDQLAVGRAQQ
                     AFNPDGTLTDTAQMAKLNAMTARLHDTLRALY"
     gene            2313..>2621
                     /locus_tag="VFX11_14405"
     CDS             2313..>2621
                     /locus_tag="VFX11_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX4939866.1"
                     /translation="MTSLIRPHEKDIGFSVKRLLPSAERQRVGPFIFLDHMGPATFAP
                     DSTANDVRPHPHIGLATVTYLFTGAMQHKDSTGVEQRIEPGAINLMTAGRGVVHSERI
                     P"
BASE COUNT          460 a          859 c          774 g          528 t
ORIGIN      
        1 ccgcccatgt cggtgcgcac ccagcccgga tgcaggatcg ccaccttgat attgtcgccc
       61 gccagatcaa tcgccagcga ttttgccgct gcgttcagcg ccgctttcga ggatcggtag
      121 atataactgc caccggacgt gttgtcgccc atcgatccca tcttgctgct gagaattgcc
      181 accgtggaca cctctgcatt cgccagattg gcgcgcagcg cttccaccat tttgatcggc
      241 gcgatggcgt tggtatgcag cacctgcagc caggcggcct cgtccagctt gcccagtgaa
      301 ccccagccgc cggatacgcc ggcgttgttg atcagcaggt gaatgggctg gccgtcgagg
      361 tcgcgcgcca gttgctggcg ggagtgcgca tcggtgacgt tcagtgtgtg cagcgtcagc
      421 agttcgggat attgtttggc cagcgcctgc agcgcagtga ccttcgccag atcgcgggcg
      481 gtggcgatga cacggttgcc ctgctccaga tactgcttcg cgaattccag gcccagtccg
      541 cgaccggcgc cggtgacaag aacggttttc atggtgtgct cctgatgact gttgtcctga
      601 tacgtgcggg gaatgtcctg cctgcgattt gacgtcagtc ggtggcaaag ggttcgtact
      661 gcacgcgtac gatttcaaac gcggcgacgc ccttggggcg ctgcaccgtg acctcatcac
      721 cctcctgctt tttcagcaag gcccgtgcca tgggggaatc cacactgatc cagccattgt
      781 ccagatcaat ctcgtccgct cccaccagac ggtagcgttc cagcgtgccg gattccactt
      841 cctccagctc cacccaggcg ccgaaaaaca cccggctctg gtccgccggt ttctgctcca
      901 ctgccttcac cgcctccagc cgtttcacca gatagcggat gcgactgtcg atttcgcgca
      961 gctgtttctt gcggtaaata tattcggcat tttcggaacg gtcaccttcg gcagcggcgg
     1021 cgctcagggc tttcaccacc tcgggccgca gcttttcact tttgtcacgc agttcggcca
     1081 tcagccggtc gcgaccttcc ggcgtgatat aaggggccga tggtttcgac ggcgggcggt
     1141 aacgggacat ggagtgattt cagttcctgc tgatcagatc gggattgctg gagagttcct
     1201 tcaccatctt gtattccacc gacagcactt tcattaccgg cccctgctgg ctcatttcca
     1261 cttcgctgcc gagcaggaag ttgaacgaca ggtagccacc ctgctccgga atgcagtggg
     1321 ggaacagcgc gatcagaccg gtgaagtagt cgcgcggtgc caccacggtg atttgcaggg
     1381 tgccatcgcg ctggcgcacc tggccgatgt gggggcccgc cagccggttg gcttcgcgaa
     1441 tctgcaccac ggcctgcttt tcagccaggt gcgaggattt gcagccgcct tccagcttgg
     1501 cgttccaggc catgccggac ttgccggttt cagggtccac tgcgagggtt tgggccagct
     1561 tgagcgtgct gacaatcatt tgaatctggg cgtcgtaatg catgatcggg gcgtcgttgt
     1621 tattgttctg atgtcagcgt gggaaaaatt ggttatacag ggcgtacaag tgcaactcta
     1681 acacgccagg gaccaaccag ggagatgaca ccgtgaccac cacccccgcc atactggttt
     1741 ttgccggcag cgcccgccgc gaatccctca acaagaagct ggcccgcgcc gccgccgaca
     1801 gtgcccgcag caccggtgct caggtaaccc tgctggatct gcgtgattac ccgattcccc
     1861 tgtacgacgg cgatctggaa gctgaaagcg gtgtgccgga aaacgcccgc gccctgcgcc
     1921 ggctgatggc cacacaccat ggcctgattg tggtgtcgcc ggagtacaac ggcttcatca
     1981 caccgctgct gaagaacacg ctggactgga tttcccgccc cgatggcgcc gaggatggcc
     2041 tgaccctgta ccgcgacaag gtggcgctgg tgctgtcggc gtcgcccggt gccctgggcg
     2101 gtctgcgcag cctgcagctg atccgccagc tgctgggcaa cctcggcgtc accgtactgc
     2161 cggaccagtt ggcagtgggc cgcgcccagc aggccttcaa cccggacggc acgctcactg
     2221 acaccgccca gatggccaaa ctcaacgcca tgacagcacg gctgcacgac accctgcgcg
     2281 ccctgtactg aacccacttc cggagccacc ccatgaccag cctgatccgc ccccacgaaa
     2341 aagacatcgg ttttagcgtc aagcgcctgc tgccgtccgc cgaacgccag cgagtaggcc
     2401 ccttcatctt tctggaccat atgggacctg ccaccttcgc gccggattcc accgccaacg
     2461 atgtgcggcc gcatccccac atcggcctgg ccaccgtgac ctacctgttc actggcgcca
     2521 tgcagcacaa ggacagcacc ggggtggagc agcgcatcga acccggcgcc atcaacctga
     2581 tgaccgcggg ccggggcgtg gtgcattcgg aacggattcc g
//
LOCUS       DASUEG010000763         6253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1122726, whole genome shotgun sequence.
ACCESSION   DASUEG010000763 DASUEG010000000
VERSION     DASUEG010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122726"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFX11_14410"
     CDS             complement(<1..753)
                     /locus_tag="VFX11_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939867.1"
                     /translation="MSAMRILSGILMLLTLAGCDSGGGGSGSSDIERTNNIPDQTDTG
                     TGGNGGTTGTPPPVTAAESNLYLPTTSGVIWHYQTDSSGEAITARFDGTTTIDGKTLG
                     NFTYSMGLTDYVLSDPAYVGYGGSEFALADPQSSDLYTFDVKLKTAHPFLGLNAGGNY
                     SGNAVISVNPGNIQVSAIPYNGSISAGSVSRIDAGDVGSLPARRVSIMLNPGGVAGDL
                     GIGAVPEFLRPLLERRTVELWLAPGIGIVQRND"
     gene            complement(938..1849)
                     /locus_tag="VFX11_14415"
     CDS             complement(938..1849)
                     /locus_tag="VFX11_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008732999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4939868.1"
                     /translation="MHIGKVDLNLYLVLDAIHREGSITRAAVSLNLTQPAVSHALARL
                     RDVYGDPLFTRTGNRMVPTPLARAILQPVRDALAGLQHTLSGPASFDPATNRKQIVLG
                     MRDIVETVLLPELVPLLEQRAPLTQVASVRVARRDMEAELGSGRLDLAFDVLLPVGPA
                     IAHTKLMEGRFVVVSCKRHPLLRGGLTLEKYLQLRHVVVSSRRSGPTVEDFELSRLGL
                     HREIGMRCQNFFVAWKTVASSRMLLTVPESHARGDLFQQNLKVWPMPVELPPLAVHMY
                     WHENLQDDPANRWLRERVLELLRKAVV"
     gene            1973..3187
                     /locus_tag="VFX11_14420"
     CDS             1973..3187
                     /locus_tag="VFX11_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4939869.1"
                     /translation="MDFSQSPKTQEMIARVKAFMNEHIIPNEQKYVEQLGEVTGDWTR
                     WTVPPLMEEWKEKAKAAGLWNLFLPDEEYGAGLNNLEYAPLAEIMGYSPFASEAFNCS
                     APDTGNMEVLVKYGSPAQKKQWLEPLLEGTIRSVFFMTEPGVASSDATNMEATCVIEN
                     DEVVINGRKWWSSGVGHPQCKIGIFMGVTDPSASRHAQHSMVLVPLDTPGVKIERMLP
                     VFNRYDAPEGHGEVSFTNVRVPVSNIIAGPGRGFEIAQGRLGPGRIHHCMRLIGASER
                     ALKHLCERATSRVAFGKPLASLGGNRDIIANSRIDIECARLLTLKAAYLMDTVGVFGA
                     MTEISAIKVVAPNVAQKVIDAAIQIHGGGGVSDDFPLAAMFGWARVLRLADGPDEVHR
                     GLIAKFELNKYK"
     gene            3208..4056
                     /locus_tag="VFX11_14425"
     CDS             3208..4056
                     /locus_tag="VFX11_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4939870.1"
                     /translation="MSQEKTVAITGGSSGLGKALALRFARAGWKVAIADINQERGEET
                     LAQLKALAPDTFYQHCDVRNASDLMQWRDAILARWQRIDVVVNNAGVATFGNIDQCSL
                     EDWDWVLDINLMGVVRGCKYFSEVFKQQGFGHIVNVASMAGLVHSPGMSSYNATKAGV
                     VALSETLRGELKPYGIDVTVVCPGFFQTNLAESTRSPDADAKQLVAKMLARSAINADD
                     IANMIFLAIEERKYLVLPHRDYRKIWYLKRFLPFLYTRLMGKIGSKMAHSQKTRKPLS
                     SEGSAA"
     gene            4053..5120
                     /locus_tag="VFX11_14430"
     CDS             4053..5120
                     /locus_tag="VFX11_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4939871.1"
                     /translation="MNTNVLDQAGNVREGEELDAKVVTDFIRAAVPDLQGTASIKQFP
                     GGASNLTYAVSFPNKEYILRRPPFGKKAKSAHDMGREYRVMQKLKPVYPYVPNMVAFC
                     QDESVLGCDFYVMERLVGIIPRADMPKGLHMTPEQNRALCLNVLDRFIELHSVDYQAA
                     GLADLGKGEGYVVRQIEGWSKRYHDAKTPDVPDFEKIMAWLHAHMPPQVKVCIIHNDY
                     RMDNVVLDAQDPTQVIGVLDWEMATLGDPLMDLGNTLAYWVEANDPPAAQLLRRQPSH
                     LPGMLNRNEVIDYYCGKMGLSTDNFVFYRAYGIFRLAVILQQIYYRFYHGQTKNPAFG
                     AFGQMVKILESQCNELLAGEI"
     gene            5125..5814
                     /locus_tag="VFX11_14435"
     CDS             5125..5814
                     /locus_tag="VFX11_14435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4939872.1"
                     /translation="MAALYLIRHGQASFGSANYDALSERGLQQGRVLGEYLVERGIRF
                     DAAFAGTMQRHAQTAEQCLGAMQCDLPLIRLAEFNEYNHEEVFARHRPELEDKTQFAQ
                     FLAQSSNPHKAFQKEFEKAMLRWRSGDYDDEYSESWLAFRTRSINALESARQHGAKSI
                     AVFSSGGPIANITGHCLGLNDDQIVELSWAVMNCSITCLLFTADKLSLRYFNDFAHFE
                     ARADKSLVTHR"
     gene            5844..>6253
                     /locus_tag="VFX11_14440"
     CDS             5844..>6253
                     /locus_tag="VFX11_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011637527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939873.1"
                     /translation="MNLKNPFDLSGRIALVTGASRGIGESIARLLAAQGAHVIVSSRK
                     VEDCQRVVNDIVSAGGKAEAYACHIGDMDQIKGIFAHIKQTHGKLNILVNNAATNPYF
                     GHVLDTDLGAFQKTVDVNIRGYFFMSVEGGKLMR"
BASE COUNT         1317 a         1894 c         1859 g         1183 t
ORIGIN      
        1 gtcattgcgc tgcacgatac caatgcccgg cgccagccag agttccaccg tgcgacgttc
       61 cagcagcggc cgcagaaatt caggcacagc accgatgccc agatcacccg ccacaccgcc
      121 gggattcagc atgatgctga cccgtcgcgc cggcagagag ccgacatcgc cggcatcgat
      181 cctgctcaca ctgcccgccg aaatactgcc gttataggga atggcgctga cctggatatt
      241 gccgggattc acgctgatca ccgcattgcc cgagtagttg ccaccagcat tgagccccag
      301 gaaagggtgc gcagttttca gtttcacatc aaaggtgtaa aggtcgctgc tttgcggatc
      361 ggccagggca aactctgaac cgccgtaacc cacgtaggca gggtcgctca gcacataatc
      421 ggtcaggccc atgctgtagg tgaaattgcc gagggtttta ccgtcgatag tggtcgtgcc
      481 gtcgaaacgg gcagtgatgg cttcgccgct gctatcggtc tggtagtgcc agatcacgcc
      541 ggatgtggtc ggcagataca ggttgctttc cgctgcggtc accggcggtg gtgtgccagt
      601 ggtgccgccg tttccgccgg taccggtgtc ggtctgatca ggaatattgt tggtccgctc
      661 gatgtcactg ctgccgctgc cgcccccgcc gctgtcacac cccgccaatg tcagcaacat
      721 caaaatgccc gataaaattc gcatcgcgct catgccgtgt tccccagctt cttgttattt
      781 ggattgtgtt gtttgaaatg atcaggggcg cctggatgcc ccgggagcga tactgtacca
      841 gattgctgcg cgcctgccgg taccatcggg cgcaaaatga cgatgctgtc cgcaatggac
      901 attgcgcaat ctgctggcag cttcatcagt catgttttta cactaccgcc ttgcgcagca
      961 attccagcac ccgctcccgc agccagcggt tggccgggtc gtcctgcagg ttttcgtgcc
     1021 agtacatgtg caccgccagt ggcggcaatt ccaccggcat cggccacacc ttcagattct
     1081 gctggaacag atcgccacgg gcatgacttt ccggcacggt gagcaacatg cgcgagctgg
     1141 caaccgtctt ccaggccacg aagaaattct ggcagcgcat gccgatttcg cgatgcaaac
     1201 ccagccggct cagctcgaaa tcctccaccg tggggccact gcgccgcgac gacaccacca
     1261 catgccgcag ctgcagatat ttttccagcg tgagcccgcc gcgcagtaac ggatgccgct
     1321 tgcaggacac caccacgaag cgcccttcca tcaatttggt gtgcgcaatt gccggcccca
     1381 ccggcagcag cacgtcgaac gccagatcca gccggccgct gcccagttcc gcttccatgt
     1441 cgcgccttgc cacccgcacg ctggcgacct gggtcagggg cgcgcgctgt tccagcagcg
     1501 gcaccagctc cggcaacagc acggtttcca cgatgtcgcg catgcccagc acgatctgct
     1561 tgcggttggt ggccggatcg aagctggccg gcccggacag ggtgtgctgc aggcccgcca
     1621 acgcatcgcg caccggctgc aggatggcgc gggccagcgg cgtgggcacc atgcggttgc
     1681 cggtgcgggt gaacagcgga tcgccataga catcgcgcag ccgcgccagg gcgtgactga
     1741 ccgccggctg ggtcagattc agactgacgg ccgcgcgggt gatggagcct tcgcggtgga
     1801 tggcgtccag caccaggtaa aggttgaggt cgaccttgcc gatatgcatg gcattgatat
     1861 cctgtaataa caactattca ttggctttat tatgcagtgt cttttagcct tgcatcaaac
     1921 ctgtcagcca atacggttac ctgtcagcca gtccggtcaa aaggagcgtt ccatggattt
     1981 cagccaatcg cccaaaaccc aggaaatgat cgcccgcgtc aaggccttca tgaatgagca
     2041 cattatcccc aacgaacaga aatacgtgga gcagctgggc gaagtcaccg gcgactggac
     2101 gcgctggacc gtgccgccgc tgatggagga atggaaagaa aaagccaagg ccgccggcct
     2161 gtggaacctg ttcctgccgg atgaggaata tggcgccggg ctgaacaacc tggaatatgc
     2221 gccgctggcg gaaatcatgg gttattcgcc gtttgcgtcg gaagcgttca actgcagcgc
     2281 gccggacacg ggcaacatgg aagtgctggt gaagtacggt tcgccggcgc agaaaaaaca
     2341 atggctggaa ccgctgctgg aaggcacgat ccgctcggtg tttttcatga cggagccggg
     2401 cgtggcctcc tccgatgcca ccaacatgga agccacctgc gtcatcgaaa acgatgaagt
     2461 ggtcatcaac ggccgcaagt ggtggagttc gggtgtcggc catccccagt gcaagatcgg
     2521 gatattcatg ggcgtcaccg atccttccgc cagccgtcac gcgcagcatt ccatggtgct
     2581 ggtgccgctg gatacgccgg gcgtgaaaat cgagcgcatg ctgccggtgt tcaaccgcta
     2641 cgacgcaccg gaaggtcacg gcgaagtgtc gttcaccaat gtgcgggtgc cggtgtccaa
     2701 catcatcgcc ggacctggcc gtggttttga aatcgcgcag ggccgcctgg gcccgggccg
     2761 cattcaccac tgcatgcgcc tgatcggcgc ctcggagcgc gcgctgaagc atctctgcga
     2821 acgtgcgact tcccgcgttg ccttcggcaa gccgctggcg agcctgggcg gcaaccgcga
     2881 catcatcgcc aattcccgca tcgacatcga gtgcgcccgc ctgctgacgc tgaaagccgc
     2941 ctatctgatg gacacggtcg gtgtgttcgg cgccatgact gaaatctctg ccatcaaagt
     3001 ggtggcgccg aatgtcgcgc agaaagtgat tgatgcggcg atccagatac acggcggtgg
     3061 cggcgtcagc gatgattttc cgctggcagc catgttcggc tgggcgcggg tgctgcggct
     3121 cgccgacggc ccggatgaag tgcatcgtgg cctgatcgcg aaatttgaat tgaacaaata
     3181 taaataattc accgaagccg ggatgttatg agtcaggaaa aaacagttgc gatcacgggc
     3241 ggcagcagtg gtctgggcaa ggcgctggcc ttgcggtttg cccgtgcggg ctggaaagtg
     3301 gcgattgccg acatcaacca ggaacgcggc gaggaaacgc tggcgcaact gaaagcactg
     3361 gcgcccgaca cgttttacca gcattgcgac gtgcgcaacg ccagtgacct gatgcagtgg
     3421 cgcgatgcga ttctggcgcg ctggcaacgc atcgacgtgg tggtgaacaa tgccggcgtt
     3481 gccaccttcg gcaacatcga ccagtgttcg ctggaagact gggactgggt gctcgacatc
     3541 aacctgatgg gtgtggtgcg cggctgcaaa tatttcagcg aagtgttcaa gcagcagggc
     3601 tttggccaca tcgtcaacgt cgcatccatg gccggcctgg tccactcacc gggcatgagc
     3661 agctacaacg ccaccaaggc cggcgtggtg gccctgtcgg aaaccctgcg cggcgaactg
     3721 aagccctacg gcatcgacgt caccgtcgtg tgcccgggct ttttccagac caacctggcg
     3781 gaatccaccc gctcccccga tgccgacgcc aagcagctgg tggcaaagat gctggcccgt
     3841 tccgccatca atgccgacga cattgccaat atgatctttt tggccattga agagaggaaa
     3901 tatctggttt tgccgcacag agactatcgc aagatctggt atctgaagcg tttcctgcca
     3961 tttctctata cccgtctgat gggtaaaatc ggcagcaaaa tggcgcatag ccaaaaaacc
     4021 agaaaaccac tgtcttcaga gggatcagca gcatgaatac aaacgtgttg gaccaggccg
     4081 gcaatgttcg cgaaggtgag gaactggatg cgaaagtcgt aaccgatttt atccgtgcgg
     4141 cagtacccga tctgcagggt accgccagca tcaagcagtt tcccggtggc gcgtcgaacc
     4201 tgacctacgc ggtatcgttt cccaacaagg aatacatcct gcgccggccg ccgttcggca
     4261 agaaagccaa atccgcccac gacatggggc gcgaataccg ggtgatgcag aagctcaagc
     4321 cggtttaccc ctacgtgccc aacatggtgg ccttctgcca ggacgaaagc gtgctgggct
     4381 gcgatttcta cgtgatggaa cggctggtgg gcatcattcc ccgcgccgac atgcccaagg
     4441 gcctgcacat gacgccagag cagaaccgcg ccctgtgcct gaacgtgctg gaccgcttca
     4501 ttgaactgca cagcgtggac taccaggccg ccggtctggc ggatctgggc aagggcgaag
     4561 gctatgtggt gcgccagatc gaaggctggt cgaagcgcta tcacgatgcc aaaaccccgg
     4621 acgtgcccga cttcgaaaaa atcatggcct ggctgcacgc gcacatgccg ccccaggtga
     4681 aagtctgcat catccacaac gattaccgca tggacaacgt ggtgctggac gcgcaggacc
     4741 cgacccaagt catcggcgtg ctggactggg aaatggcgac gctgggcgat ccgctgatgg
     4801 acctgggcaa caccctggcc tactgggtgg aagcgaacga tccgcccgcc gcgcaattgc
     4861 tgcgccgcca gccgtcgcac ctgccgggca tgctcaaccg caacgaagtg atcgactatt
     4921 actgcggcaa aatgggcttg agcaccgaca atttcgtgtt ctaccgggcc tacggcatct
     4981 tccgtctggc ggtgatcctg cagcagatct attaccgctt ctaccacggc cagaccaaaa
     5041 atccggcctt cggtgcgttc ggccagatgg tgaaaatcct ggaaagccag tgcaacgaac
     5101 tgctggctgg cgagatctga tccgatggcg gcgctctacc tgatccggca tggccaggca
     5161 tccttcggca gcgccaatta cgacgctctc agcgaacgcg gcctgcaaca gggccgcgta
     5221 ctgggggaat atctggtgga gcgcggcatc cggtttgatg ccgcattcgc cggcaccatg
     5281 caacgccacg cgcaaaccgc agagcaatgc ctgggcgcca tgcaatgcga tttgccgctg
     5341 atccggctgg cggagttcaa cgaatacaat cacgaggaag tgttcgcgcg ccaccggccc
     5401 gaactggaag acaaaacgca gttcgcccag tttctggcgc agtcatccaa tccgcacaaa
     5461 gccttccaga aggaattcga gaaagccatg ctgcgctggc gcagtggcga ttacgacgac
     5521 gaatattccg aatcctggct ggcgtttcgc acccgcagca tcaatgcgct ggaatccgcg
     5581 cgccagcacg gcgccaaatc catcgccgtg ttttcctccg gcggccccat cgccaacatc
     5641 accggccact gcctgggcct gaacgacgat cagatcgtgg agttgagctg ggccgtcatg
     5701 aattgctcga tcacctgtct tttgttcacc gcagacaaac tcagtctgcg ctacttcaat
     5761 gacttcgccc attttgaagc ccgcgcggac aagtcattgg tgacccatcg ctaggcaaca
     5821 caataaagaa agaggcaatt ccgatgaacc tgaaaaatcc gtttgatctg tccggccgca
     5881 tcgcactggt gaccggcgct tcccgtggca ttggcgaaag cattgccaga ctgctggcgg
     5941 cccagggcgc ccatgtgatc gtgtccagcc gcaaggtgga agactgccag cgcgtggtga
     6001 acgacatcgt cagcgccggt ggcaaagctg aagcctatgc ctgccatatc ggcgacatgg
     6061 accagatcaa ggggattttt gcgcacatca aacagaccca cggcaaactg aacattctgg
     6121 tgaacaacgc ggcgaccaat ccctactttg gccatgtgct ggacacggat ctgggcgcat
     6181 tccagaaaac cgtggacgtg aatattcgcg gctatttctt catgtcggtg gaaggcggca
     6241 agctgatgcg cga
//
LOCUS       DASUEG010000764         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_715987, whole genome shotgun sequence.
ACCESSION   DASUEG010000764 DASUEG010000000
VERSION     DASUEG010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715987"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFX11_14445"
     CDS             <1..342
                     /locus_tag="VFX11_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939874.1"
                     /translation="HSGFYGGMIGLGIGVLFALYLTASELGQYIGVGTFIFVCCIFTF
                     FGAWTGGIVGISHENHHIARFHDALEQGDTLLMLDAYSPQQENRVRNLMHSRHLEATF
                     EGEDKDYREFV"
     gene            complement(521..868)
                     /locus_tag="VFX11_14450"
     CDS             complement(521..868)
                     /locus_tag="VFX11_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4939875.1"
                     /translation="MSTNPERHVDVSALQDLKEIMESEFETLIDTFIADSTSKLEELA
                     LAIAASDADALRKVAHSLKGSSSNICAGELSELARQLEFMGKEGTTSGAAAILQQLRG
                     EFTTVAAILQGNI"
     gene            complement(886..2631)
                     /locus_tag="VFX11_14455"
     CDS             complement(886..2631)
                     /locus_tag="VFX11_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEX4939876.1"
                     /translation="MDTAPLTVASSEEPQEVLRILIADDNDSDRLILQTIVRKEGHTV
                     YTARDGQEAIDIFREQSPDIVLLDALMPNVDGFEAARTIKALAGDDLVPIIFLTSLRD
                     ATSLADCLAAGGLDFLSKPYNRIILQAKIKAFASMRRLHETVQRQRDEISLHNDRLIH
                     EQEVAKQVFDNIAHPGCIDAPNIKYLLSPMAVFNGDLVLASRKPSGGMYVFLGDFTGH
                     GLPAAIGAMPVSEIFYGMAQKGFSLQDILREINKKLKRILPVGVFCCACMYDLSFRKR
                     SAEVWVGGLPDILVYRKQTRVIENLPSRHLPLGVVDNDRFKTETTVIPLDFGDKIFLW
                     SDGIIEATNDQGEMFGEDRVRDVIASTPDAEAIFSAIEAAVEKHAGRGEQDDDYTMVE
                     VTMVNEEDMGEYFADRGKSFLAGGPKDWSMTYELRPQTLREFNPLPLITHLLMEVPGL
                     RPHSGKIYTILSELYSNALEHGVLGLQSDLKASADGFARYYLERTKKLSDLYEGSVKF
                     HIDHHPVKDGGVLTIVVEDTGPGFDFKAREQASHRTEGYSGRGIPLIRTMCRSVRYLE
                     PGNKVEVVFDWHVDV"
     gene            complement(2681..2983)
                     /locus_tag="VFX11_14460"
     CDS             complement(2681..2983)
                     /locus_tag="VFX11_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4939877.1"
                     /translation="MAIWSTEASSGELTIKIQGRFDFSAHQEFRDAYEKVSEEPKGYI
                     VDMKETTYLDSSALGMLLLLRDHAGGDNSHIEIVNCNPDVKKILTISNFEQLFTIR"
BASE COUNT          613 a          875 c          862 g          639 t
ORIGIN      
        1 cacagcgggt tttacggcgg catgatcggg ctgggtattg gcgttctgtt tgcgctgtac
       61 ctgaccgcat cggaactcgg ccaatacatc ggcgtgggca ctttcatctt cgtatgctgc
      121 attttcacct tcttcggcgc ctggacgggc ggaatcgtcg gcatctcgca cgagaaccat
      181 catatcgccc gcttccacga tgcgctggaa cagggcgaca ccctgctgat gctggatgcg
      241 tattcgccac aacaggaaaa ccgtgtccgc aacctgatgc attcgcgcca tctggaagcg
      301 accttcgaag gagaagacaa ggattaccgc gaattcgtgt gaacaggttt gagagtggcc
      361 gggccccgcc ggctatcggt gcagtcagaa cccatcaatc acaacagcga gagctagaca
      421 acatcatcag gacaccaccc ggcgtccgaa ccttgcaaaa acaccgacag cccacccggc
      481 cgctccctcc acgagcggcc ttttcatttc tggtccagga tcagatattt ccctgcagaa
      541 tggcagcaac tgtggtgaat tcaccccgca gctgttgcag gatcgccgcc gcaccactgg
      601 tggtaccttc cttgcccatg aattccagtt gacgcgccag ctccgacaac tcgcccgcgc
      661 aaatattgct ggaggatccc ttgaggctgt gcgcgacctt gcgcagggca tcggcatcgc
      721 ttgcagcaat cgccagcgcc agttcctcga gcttgctggt ggaatccgca atgaacgtgt
      781 cgatcagcgt ttcgaattcg ctttccatga tctctttcag gtcctgcagc gcactgacat
      841 ccacatgccg ttcgggattc gtactcatac cttgatcctt atcgtttaaa cgtcaacatg
      901 ccagtcaaac accacttcca ccttgttgcc gggctccaga tagcgcacag agcggcacat
      961 ggtgcggatc aggggtatac cccgcccgct atagccttct gtcctgtggc tggcctgctc
     1021 ccgtgccttg aaatcaaaac cgggtccggt gtcctccacc acgatggtga gcacacctcc
     1081 gtccttgacc gggtggtgat cgatatggaa ttttaccgaa ccttcataca gatccgacag
     1141 ttttttggtg cgctccagat aataacgggc aaaaccgtcg gcagaggcct tcagatccga
     1201 ttgcagaccg agcacgccgt gctccagggc attggaatac agctccgaca gaatcgtgta
     1261 gatcttgccg ctgtgggggc gcaggcccgg cacttccatc agcaggtggg tgatcagtgg
     1321 cagcgggttg aattcgcgca gggtctgcgg ccgcagctca taggtcatgc tccagtcctt
     1381 cgggccgcca gcgaggaagg atttgccacg gtcggcaaaa tattcgccca tgtcctcttc
     1441 attgaccatg gtgacttcca ccatggtgta gtcgtcgtcc tgctcgcccc ggcccgcatg
     1501 cttttccacg gcggcctcga tggcagaaaa gatggcctcg gcatccggcg tggaggcgat
     1561 gacatcgcgc acccggtctt caccgaacat ttcaccctgg tcattggtgg cttcgatgat
     1621 gccgtcggac caaagaaaga tcttgtcgcc gaagtccagg ggaatgacgg tggtctcggt
     1681 cttgaagcgg tcgttgtcca ccacacccag cggcaggtga cgggacggca ggttctcgat
     1741 gacgcgggtt tgcttgcggt agaccaggat gtcgggcagg cctcccaccc agacttcagc
     1801 ggagcgcttg cggaagctga ggtcgtacat gcaggcgcag cagaacacgc ccacgggcag
     1861 gatgcgcttc agctttttat tgatctcgcg caggatgtcc tgcagcgaga accccttctg
     1921 cgccatcccg taaaagattt ccgacaccgg catggcacca atcgctgccg gcagaccgtg
     1981 gccggtgaaa tcccccagga acacgtacat gccaccggag ggcttgcgcg aggccagtac
     2041 cagatcgccg ttgaacacgg ccatgggtga cagcaggtac ttgatgttgg gggcgtcgat
     2101 gcagccgggg tgcgcgatgt tgtcgaacac ctgcttggcc acttcctgct cgtggatcag
     2161 gcggtcattg tgcagggaaa tctcgtcgcg ctgacgctgc acggtttcat gcagacgacg
     2221 catgctggca aaggccttga tcttggcctg caggatgatg cggttgtagg gcttgctcag
     2281 aaagtccagg ccaccggccg ccagacagtc tgccagcgag gtggcgtcac gcagcgacgt
     2341 caggaaaatg attggaacca gatcgtcacc cgccagtgcc ttgatggtgc gcgccgcctc
     2401 aaagccgtcg acattgggca tgagggcgtc cagcagcacg atgtcggggg attgctcgcg
     2461 gaaaatgtcg atggcttcct ggccgtcacg ggcggtatag acggtatgac cttccttgcg
     2521 gacgatggtt tgcaggatca gtcggtcgga atcattgtcg tcggcaatca ggatgcgcag
     2581 gacttcctgc ggttcctcgg aggatgcaac agtcagcggg gcggtatcca tgggtggggg
     2641 ctctctgcac ccgccggaaa gcgaatgcag agaaagcggt tcagcggata gtgaacaact
     2701 gctcgaaatt cgaaatcgtg aggattttct tcacgtccgg attgcagttg acaatctcga
     2761 tgtgggagtt gtcaccaccg gcatgatccc gcagcagcaa cagcatgccc agcgcggaac
     2821 tgtccaggta ggtggtttcc ttcatgtcca ctatatagcc tttgggttct tccgatacct
     2881 tttcgtaagc gtcccgaaat tcctgatgag cactgaaatc gaaacgaccc tgaatcttga
     2941 tcgtcagttc acccgaggat gcttcagtag accaaatcgc cattatcta
//
LOCUS       DASUEG010000765         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2107771, whole genome shotgun sequence.
ACCESSION   DASUEG010000765 DASUEG010000000
VERSION     DASUEG010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107771"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /gene="traD"
                     /locus_tag="VFX11_14465"
     CDS             <1..1302
                     /gene="traD"
                     /locus_tag="VFX11_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261889.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HEX4939878.1"
                     /translation="AGQMPGDGQSQAFREFVWRYVNVISSCRNALGERPGIEQIKYDA
                     ENLEPLVIEYLEFVFERECPADGPLGDWRKTIAKWEPLYNDPDEKDFRKPRHMTDRSN
                     RAMALCKFYKDNKAKHRNLEDTCAESLITTFFYDAAKFNMLVASLMPLLEKLCTGKCA
                     ELISPDYLDGDRNRPIFTWSEVIRTGGIVYVGLDALSDPEVAAAVGNSMFADLTSGSG
                     LIYKHGVDLGLPFSTGGRRVYIHADEFNELIGKEFIPLLNKAGGSGYAVTAYTQTWSD
                     VIARLGNQAQAGQVAGNLNTLIMLRTLEYATIEMLTKRLRQVDVSHAMDVSGASDVSD
                     PDANTSFTSRTEQRVTSQRIDRIHPNDVTQLPKGQAFSLTDGGKLSKIRLPMPDKADL
                     ADLPPTLQDMASDMATRYKSDANWYHFQPCFEVPGDFNGLN"
     gene            1238..2098
                     /locus_tag="VFX11_14470"
     CDS             1238..2098
                     /locus_tag="VFX11_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HEX4939879.1"
                     /translation="MPTGTTSSLALKCREISMASTNAAAQAPVAPQPWHLRLLWSPLA
                     LLGMLFGALVSAVMIEWVGMATGFWDEPGAEHARQTLVQDIQYLNADFTRSILGISPV
                     DLSARAAQSVSQSVADTVERLQLPQIYQQLKAEGSGIFSKIMKMQQLPGPHSEPGTSV
                     NRSFPVDADAALTAFMTYLDAGLYSVQTVVIRVVVALLTLPAYLLIGMACLLDGVVAR
                     DLRKFTAANESAFAYHRYRPWAKRCFVAGWYLYIAWPGPIHPNVVFVPSALLCGLAIF
                     NTGKWFKKFF"
     gene            2259..>2504
                     /locus_tag="VFX11_14475"
     CDS             2259..>2504
                     /locus_tag="VFX11_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HEX4939880.1"
                     /translation="MKGPINLKKKLATTVMLIGSVFAMPLQADNAAPLAVSAAELERT
                     RLAEIVRGIDQLMLQVDQASRTGASGRVQFNYDALRRD"
BASE COUNT          562 a          665 c          702 g          575 t
ORIGIN      
        1 gcaggtcaga tgccagggga tggccaatcg caagcttttc gtgaattcgt gtggcgctat
       61 gtcaatgtga tttcgtcttg tcgcaatgcg ttgggtgaac gtcctggtat tgagcagatc
      121 aaatatgacg cggagaatct agagccactg gtgatcgagt acctggaatt cgtatttgag
      181 cgtgaatgcc ccgccgatgg gccgttgggt gattggcgta agaccattgc caaatgggag
      241 ccgctctaca acgatccgga tgaaaaggat tttcgcaaac cgcgccatat gaccgatcgc
      301 agtaatcgag ccatggcgct gtgcaaattc tacaaggaca acaaggctaa acatcggaat
      361 ctggaagaca cctgcgctga atcactgatc accacattct tttatgacgc ggccaagttc
      421 aacatgctgg tcgcctcgtt gatgcccctc ctggaaaaat tgtgtacggg aaaatgtgcg
      481 gagctgatca gccccgacta tctggatggt gatcgcaacc ggcctatctt cacctggtct
      541 gaggtgattc gcacgggtgg cattgtgtat gtgggtctgg atgcgttgtc tgatccggaa
      601 gtcgcggcag cggtcggcaa ctccatgttt gccgacctga cttcgggatc aggtttgatt
      661 tacaagcacg gcgtggatct tgggttgccg ttttccactg gtggtcgacg tgtctatatc
      721 catgccgatg aattcaacga actcatcggc aaggaattca ttcctcttct gaataaggca
      781 ggtggttcgg ggtatgcagt aacggcctac acccaaacct ggtcagatgt gattgcccga
      841 ctggggaatc aggcgcaagc agggcaggtg gccggcaacc tgaatacttt gatcatgctg
      901 cggacgctgg aatacgccac catcgaaatg ctcaccaagc gattgcgcca ggtggatgtg
      961 tcacatgcca tggatgtttc gggggcctcg gatgtcagtg accctgatgc caacacgtca
     1021 ttcacctcgc gtaccgaaca gcgcgtcacg tcacagcgca tcgatcggat tcacccgaat
     1081 gacgtgacgc aattacccaa aggccaagcg ttttcgctca cggatggcgg caagctctcg
     1141 aaaattcgct tgcccatgcc ggataaagcg gatctggcag atttgccacc gaccttgcaa
     1201 gacatggcca gtgatatggc cacgcgatac aagtccgatg ccaactggta ccacttccag
     1261 ccttgctttg aagtgccggg agatttcaat ggcctcaact aatgcagctg cacaggcacc
     1321 ggttgcaccg cagccttggc atcttcggtt gctctggtcg cccttggcct tgctggggat
     1381 gctgttcggg gcgctggtgt cggcggtgat gatcgaatgg gtgggtatgg ctacgggatt
     1441 ctgggatgaa cctggggcag aacatgcacg acagacactg gtacaggaca tccaatattt
     1501 aaatgcggat tttacccgat ccattctcgg catttcgccg gtggatctgt cggcccgcgc
     1561 cgcgcagtca gtgtcgcagt cggtcgcaga caccgttgag cgtcttcagc tgccgcagat
     1621 ttaccagcag ttgaaagcag aaggatcagg catattttcc aagatcatga aaatgcagca
     1681 gctgccaggc ccacactctg agccgggtac gagcgtgaac cgcagtttcc ctgtcgatgc
     1741 cgatgcagcg ttgaccgcct tcatgaccta cctggatgcg ggtctatatt ctgtgcaaac
     1801 cgtcgtcatc agagttgttg tcgcgctgct cacattacct gcctacctcc tgattggaat
     1861 ggcgtgtctg ctcgacggtg ttgtggcgcg tgatctgcgc aaatttactg ccgccaacga
     1921 atccgccttt gcctatcacc gctatagacc ctgggcaaag cgctgctttg tggccggctg
     1981 gtacctgtat atcgcctggc cggggccaat ccatcccaat gtggtattcg tgcccagcgc
     2041 gttgctgtgt ggactggcca tcttcaatac cggtaagtgg ttcaagaagt ttttctgaca
     2101 aacaaagccg tacacagaca gtacttttaa ctggtcaatt aaagatcacg aagccttcag
     2161 aatcatttcg ggcgacacag attgaaaaaa agtggacaaa tgcgcttcgc cacttctgtc
     2221 gaactccaga accgacgtgg caagagcagg agactcatat gaaaggccca atcaacctga
     2281 aaaaaaagct ggccacgaca gtgatgctga tcggcagtgt ctttgcaatg ccgctccagg
     2341 cggacaatgc ggcaccgctt gccgtatccg ctgcagagct ggaacggacc cgcctcgcag
     2401 aaatcgtgcg tgggatcgat caactcatgt tgcaggtgga tcaagcctcc cgcacgggtg
     2461 cctcggggcg agtgcagttc aactacgacg ccctgcgccg tgac
//
LOCUS       DASUEG010000766         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1899091, whole genome shotgun sequence.
ACCESSION   DASUEG010000766 DASUEG010000000
VERSION     DASUEG010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899091"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /gene="pyrE"
                     /locus_tag="VFX11_14480"
     CDS             <1..120
                     /gene="pyrE"
                     /locus_tag="VFX11_14480"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458955.1"
                     /note="involved in fifth step of pyrimidine biosynthesis;
                     converts orotidine 5'-phosphate and diphosphate to orotate
                     and 5-phospho-alpha-D-ribose 1-diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX4939881.1"
                     /translation="FGIPVISIVKLEQIITYLEETGSEHLDAIRAYRAQYGIN"
     gene            153..1103
                     /gene="gshB"
                     /locus_tag="VFX11_14485"
     CDS             153..1103
                     /gene="gshB"
                     /locus_tag="VFX11_14485"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX4939882.1"
                     /translation="MNIAVVMDPIGHIKIKKDSTIAMLWAAAARGHTLWYLEQHDLFV
                     RDGVVMGNMSPLQVFEDSNRWFELGEPVARPLADMNVILMRKDPPFDMNFIYTTYLLE
                     KAEQDGVLVVNRPQSLRDCNEKLFATWFPQCMVPTLVTARIPMLREFVAEQQDVIIKP
                     LDGMGGSGIFHLQQGGKNIGSILETLTQLGTLPIMAQRFIPEISAGDKRILMIDGEPV
                     PYCLARIPQGDETRGNLAAGGRGEARPLSDSDRRIAAIVGPELRRRGLLFVGLDVIGD
                     YLTEINVTSPTCIREIDAAYGYGIADKLIAAIEEKVAARH"
     gene            complement(1202..2461)
                     /gene="rho"
                     /locus_tag="VFX11_14490"
     CDS             complement(1202..2461)
                     /gene="rho"
                     /locus_tag="VFX11_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4939883.1"
                     /translation="MNLTELKKKPIAELVKIAEEMGLENMARNRKQDVIFAILKAHAR
                     SGEDIFGDGVLEILSDGFGFLRSSEGSFLAGPDDIYVSPSQIRRFNLRTGDTIAGTIR
                     PPKEGERYFALLKVNHINFDAPENAKNKILFENLTPLFPTQRLVMELGNGSTEDLAAR
                     VIDLIAPIGKGQRSLVVAPPKAGKTMLLQNIANSITRNNPEVFLIVLLIDERPEEVTE
                     MQRTVRGEVVASTFDEPPARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLTIIATALIETGSKMDEVIFEE
                     FKGTGNQEINLDRKIAEKRVFPAFNIKKSGTRREERLMGEDELKKVWILRKLLHPMDE
                     IAAIEFLLDKMKDSKTNEEFFEMMKRK"
     gene            complement(2686..3015)
                     /gene="trxA"
                     /locus_tag="VFX11_14495"
     CDS             complement(2686..3015)
                     /gene="trxA"
                     /locus_tag="VFX11_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX4939884.1"
                     /translation="MANELIVNATDASFESDVLKAGGAVLVDYWAPWCGPCKMIAPIL
                     DELATEYQGRLTIVKVNVDDNPGTAQKYGVRGIPTLTLFKDGAVEATKVGALSKSQLT
                     AFLDSNI"
     gene            3177..>4109
                     /gene="ppx"
                     /locus_tag="VFX11_14500"
     CDS             3177..>4109
                     /gene="ppx"
                     /locus_tag="VFX11_14500"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX4939885.1"
                     /translation="MPRTPRLREDGLIAAVDMGSNSFHLAVARLDHGELKLVEGLSEK
                     VRLGGGLDDQDRLTEEAQARALACLSRFAQHLAGISPDRLRVVGTNALRVARNAPAFL
                     KKAEKILNHPIEVIAGREEARLIYLGVAHTLASQDRRLVIDIGGGSTELIIGERFEPI
                     LLESTYMGCVSYASRFFADGTISHKAMDRAITAARQELAPLLENYFNAGWDAVVGSSG
                     TFKAMRQVAVALGLDKGSGLLTAEAVRELYRMVLKLNHVNDIALPGIKEDRKAVLPAG
                     LAIVVALFDELRIDAMEYSDGALREGVLHDMIGRP"
BASE COUNT          826 a         1241 c         1230 g          812 t
ORIGIN      
        1 ttcggcattc cggtcatctc catcgtaaag ctggaacaga tcatcacgta cctggaagaa
       61 accggcagcg agcatctcga cgccattcgc gcctaccgcg cgcagtacgg catcaactaa
      121 agcccgcgac cgaccggaaa aacgccatgt cactgaacat cgccgtcgtg atggacccca
      181 tcgggcacat caagatcaag aaggactcca cgattgcgat gctgtgggcg gctgcggccc
      241 gcggacacac gctctggtat ctggagcagc acgacctgtt tgtgcgtgac ggcgtggtga
      301 tgggcaacat gtccccgcta caagtgttcg aggacagcaa cagatggttc gagcttggcg
      361 agcctgtggc acgaccgctg gccgacatga acgtgatcct gatgcgcaag gatccgccct
      421 tcgacatgaa cttcatctac accacgtacc tgctggaaaa ggccgagcaa gacggcgtgc
      481 tggtggtgaa ccgtccgcag tccctgcgtg actgcaacga aaagctcttt gccacctggt
      541 ttccacaatg catggtgccg acgctggtca ccgcccgcat tcccatgctg cgcgagttcg
      601 tggctgaaca gcaggatgtg atcatcaagc ctctcgacgg catgggcggc tcaggcatct
      661 ttcacctgca gcagggcggc aagaacatcg gctccatcct ggaaacgctc acccaactgg
      721 gcaccctgcc catcatggca cagcgcttca ttccggagat cagtgccggc gacaagcgca
      781 tcctcatgat tgatggcgaa ccggtaccct attgcctggc gcgcattccg cagggcgatg
      841 aaacccgtgg caacctggcc gccggcggcc gtggcgaagc gcgtccgctc agcgacagcg
      901 accggcgtat tgccgcaatc gtgggccccg agttgcgccg gcgaggcctg ctgtttgtag
      961 ggctggacgt gatcggtgac tacctgacgg aaatcaatgt caccagcccg acctgcattc
     1021 gcgaaatcga tgccgcttac ggttacggca tcgccgacaa gctgattgcg gccattgaag
     1081 aaaaggtagc tgcacgccac tgaggcccgc acctgttcca gcgccccacc cctgaagacc
     1141 ataaaaaaag cccgcgctac tcgcataaaa gcagcgcggg ctttcttatg agccggaaag
     1201 tttatttccg cttcatcatc tcgaagaatt cctcgttggt cttggaatcc ttcatcttgt
     1261 cgagcaggaa ctcgatggcg gcaatttcat ccatcgggtg cagcagcttg cgcaggatcc
     1321 acaccttctt cagttcgtcc tcgcccatca gtcgctcttc gcggcgggtt ccggacttct
     1381 tgatgttgaa cgccgggaac acgcgctttt ccgcaatctt gcggtcgagg ttgatttcct
     1441 ggttaccggt gcccttgaac tcttcgaaga tcacttcgtc cattttggaa ccggtttcga
     1501 tcagcgccgt ggcgatgatg gtgagcgaac caccttcctc gatattacgg gccgcgccga
     1561 agaaacgctt cggacgttcc agcgcatgtg cgtcgacacc gccggtcagc accttgccgg
     1621 agctcggaat cacggtgttg tacgcacgcg ccaagcgggt aatggagtcg agcaggatga
     1681 ccacatcctt cttgtgctcc accagacgct tggctttttc aatgaccatc tccgccacct
     1741 gcacgtggcg ggcgggcggt tcgtcgaacg tggaggcaac cacttcgccg cgcacggtgc
     1801 gctgcatttc cgtcacttct tccgggcgct cgtcgatcag caggacgatc aggaacactt
     1861 ccggattgtt gcgggtgatg gaattggcga tgttctgcaa gagcatggtc ttgccggcct
     1921 tgggaggtgc caccaccagt gagcgctgac ccttgccgat cggcgcaatc aggtcaatga
     1981 cacgcgccgc caggtcttcg gtcgaaccgt tgcccagctc catgaccaga cgctgcgtcg
     2041 ggaacaacgg cgtcaggttt tcgaacagga tcttgttctt ggcgttttcc ggtgcatcaa
     2101 agttgatgtg attgactttg agcagcgcga aataacgctc gccttccttc ggcgggcgga
     2161 tcgtgccggc gatggtatcg cccgtacgca ggttgaagcg gcggatctgc gaggggctga
     2221 cgtagatgtc gtccggaccg gcgaggaagg aaccttccga ggagcggagg aagccgaagc
     2281 cgtcggagag gatttccaga acgccatcac caaagatgtc ttcaccgctg cgggcgtggg
     2341 ccttcaggat ggcaaaaatc acgtcctgct tgcgattgcg ggccatgttt tccaggccca
     2401 tttcctcggc aatcttgacg agttcggcaa tcggcttttt tttgagttcg gtcagattca
     2461 tagaaaatcg gagaatcggg aagaagagag gggcaacgca acggcgccag cgaccagaaa
     2521 ggtctttgtt cttgaatcag tgttcttgaa tcagcgcgtg gagaacagaa acagtcggga
     2581 gatcactacc tggaactgca gaagggcgga tcgggctgtt ttccggaatg gaaccggacg
     2641 gcgtgcgttg aacgcgcggg ctgccaggca gcccgcgtgg tccgattata tattggaatc
     2701 cagaaacgcc gtcaactgcg acttggacag ggcgcccacc ttggtggcct cgacagcacc
     2761 gtccttgaac agggtcaggg tcgggatgcc gcggacgcca tatttctggg ctgtgccggg
     2821 attgtcatcc acgttcacct tcacgatggt gaggcgaccc tggtactcgg tggccagctc
     2881 atccaggatg ggggcaatca tcttgcaggg gccacaccac ggtgcccagt agtcaaccag
     2941 cacggcaccg ccggccttca gtacatcaga ctcgaagctg gcatcagtgg cgttcacgat
     3001 cagttcgttg gccattcgtt gtctccgcag gacttccaga aaacccggat gatagcagag
     3061 ccggaaccga ttgactgccc gccggacaca tcaggcggca gaacctgcgt cgcccctctc
     3121 cgccagcgac tggctgcgct accattacgt ccctgaacct gaccgagaca aaggatatgc
     3181 cgcgtacccc ccgcttgcgc gaggacggcc tgattgctgc cgttgacatg ggctccaaca
     3241 gctttcacct ggccgtcgcc cgactggacc atggcgagct caagctggtg gagggcctgt
     3301 ccgagaaggt gcgactgggc ggagggctcg acgaccagga ccgtctcacg gaggaggcac
     3361 aggcccgggc actggcctgc ctgtcgcgtt ttgcccagca tctggccggc atctcacctg
     3421 accgcctgcg cgtcgttggc accaacgcac tgcgggtggc ccgcaatgcg ccagcgttcc
     3481 tgaaaaaggc cgaaaagatc ctcaaccatc cgattgaagt gatcgccgga cgcgaagagg
     3541 cccgcctgat ctacctgggg gtggcccaca cattggccag ccaggatcgc cgcctggtca
     3601 tcgacatcgg gggcggctcg accgagctga tcatcggcga acgctttgaa cccatcctgc
     3661 tggaatccac ctatatgggc tgcgtgagct atgcctcgcg cttctttgca gatggcacca
     3721 tcagccacaa agccatggat cgtgccatca cggcagcgcg acaggagctg gcaccactcc
     3781 tggagaacta cttcaatgcc ggctgggacg ctgttgtcgg ctcctccggc accttcaagg
     3841 ccatgcgcca ggtggccgtt gccctgggac tggacaaggg ctcgggcctg ctgacggccg
     3901 aggctgtgcg cgagctgtac cggatggtgt tgaagctcaa ccacgtcaac gacatcgcac
     3961 tgccgggcat caaggaagac cgcaaggccg tattgcctgc cggactggcc attgtggtgg
     4021 ccctgtttga cgagttaagg attgacgcca tggaatattc ggacggtgcc ctgcgcgaag
     4081 gggttctgca cgacatgatc ggccgccca
//
LOCUS       DASUEG010000767         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1975495, whole genome shotgun sequence.
ACCESSION   DASUEG010000767 DASUEG010000000
VERSION     DASUEG010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975495"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VFX11_14505"
     CDS             <1..944
                     /locus_tag="VFX11_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HEX4939886.1"
                     /translation="VMAMVAACVLTPGVYFALNSPAALIGTTPEQAAQVISSWGFVVT
                     PEMLTLTAEQIGENTILSRAGGAPTLAVGMAQIFSSVFGGPGMMAFWYHFAILFEALF
                     ILTTLDAGTRVGRFMIQDMLAPVFPALGHTHSWTANLLATSLCVAGWGFFLYQGVIDP
                     LGGINTLWPLFGIANQMLAVMALLFVTVMLVKMQRGWMVLVTAVPALWLLIVTLTGSW
                     HKLFSPDVRVGFLSHADKFGAALDQGQVLAPAKSLEDMSRIVFNDQVDAALCALFAFC
                     VISMTLVSVRACWRIWRGQPSQLKEAPAQEFALGGGA"
     gene            941..1159
                     /locus_tag="VFX11_14510"
     CDS             941..1159
                     /locus_tag="VFX11_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEX4939887.1"
                     /translation="MSLKPQSGVARKAGLAQWREALDRLLGLPNYERYLDHMREAHPG
                     LPVLSRRAFYDEAQKSRYESGSDRPRCC"
     gene            1411..3756
                     /locus_tag="VFX11_14515"
     CDS             1411..3756
                     /locus_tag="VFX11_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4939888.1"
                     /translation="MMENSRFVKTCEHLVFTYRAWYVVAFFLLTIFFGYFAVHLKMDS
                     SLEKMVPMHHEYIQNWFKHKDDLSLGNDLRIAVEVTEGDIFNAEFLEVLRLVTDEAFY
                     IEGVDKSKIQSLWTPDVRWIEVTEEGFRGGEVIPPDYDGSEASLKVVQTNVLRSGQVG
                     RLVGDNFKSAIVYVPLLFLDASKGQKHDYRKLSHALEEKIRDRFQNDKIKIHIVGFGK
                     KIGDMMDGAIEVATFFIIALVLTFLLLLMDTRCLRSSLVVTLTSVSAVIWQMGIITLL
                     GYGLDPYSMLVPFLIFAIAVSHSVQIVQSFAVARAEGQENLPAARTVFRSLFAAGASA
                     LLCDAIGFYSLIIIDIRVIKELAIAAGAGTLVVTFTNLILVKVLFSYTGVSQAAINQV
                     LRKRSKPQKAWLFLSKFADPRIAAVSILIAAILGGGSFYKAKDLKTGDLDPGAPELWP
                     DSRYNRDDAFVTSNYSVSADVLVVMVETQPDKCTSYPVVEAMDRFMWHMENVPGVQSA
                     VSLVTVSKRMLAGVNEGNLKWQQLTKVEEILNNSMPSDMVSSLMNVSCSLAPVIVFLQ
                     DHKAETLKAVTRAAEDFARENDNPEVARFVLATGNAGVEAATNQTVERAASTMMWLVY
                     GIVIIMCWLTFRFSIIAVICVITPLVLTSLLCEALMAVFGMGLKVGTLPVIALGVGIG
                     VDYGIYIYSKMMEFMAKGQDLREAYFNTLCVTGKAITFTAVSLALCVGTWAFSDIKFQ
                     ADMGILLTFMFVWNMVGAMWLLPAMADFLFKFQKKKAVAKA"
     gene            4118..>4498
                     /locus_tag="VFX11_14520"
     CDS             4118..>4498
                     /locus_tag="VFX11_14520"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX4939889.1"
                     /translation="MTQDSRLIRLTANFIDDSCVAVLLVGRESLSQPYQFQIEFQSTI
                     STLLSQDVIGQKVSVAIHPQGILQPHFLHGRVNQLQLQDVTDNGTRVYQISLVPGLWF
                     LSQAGTNRIFENKTSIDIAQEIFKA"
BASE COUNT          865 a         1215 c         1399 g         1019 t
ORIGIN      
        1 cggtgatggc gatggtggcg gcctgcgtgc tgacccccgg cgtgtatttc gcgctgaaca
       61 gcccggcggc gctgatcggt accacgccgg agcaggcggc ccaggtgatc agcagctggg
      121 gctttgtggt gacgccggaa atgctgacgc tcaccgccga gcagatcggc gagaacacca
      181 ttttgtcccg cgctggcggt gcgcccaccc tggcggtagg catggcgcag atcttcagca
      241 gcgtgtttgg tggtccgggc atgatggcct tctggtacca cttcgcgatc ctgttcgaag
      301 ccctgttcat tcttactact ctggatgcgg gcacccgtgt cggccgcttc atgatccagg
      361 acatgctggc gccggtgttt cccgcgctgg gccacactca ttcctggacc gccaacctgc
      421 tggccacctc cctctgcgtg gcgggctggg gcttcttcct gtatcagggg gtgatcgatc
      481 cgctgggggg catcaacacc ctgtggccgc tgttcggcat cgccaaccag atgctggcgg
      541 tgatggcgct gctgttcgtt actgtgatgc tggtgaaaat gcagcgcggc tggatggtgc
      601 tggtgacggc agtgccggca ctgtggctgc tgatcgtgac cctgaccggc agctggcaca
      661 agctgttcag cccggatgtg cgggtgggct tcctgtccca cgccgacaaa ttcggcgcgg
      721 cgctggacca gggccaggtg ctggcaccgg ccaagtcgct ggaggacatg tcccgcatcg
      781 tgttcaacga tcaggtggac gcggccctgt gcgcgctgtt cgcgttctgc gtcatcagca
      841 tgacgctggt gtcggtgcgg gcctgctggc ggatctggcg cgggcaaccg tcgcaactga
      901 aggaagcgcc ggcgcaggaa tttgcactgg ggggtggggc atgagtctga aaccgcagtc
      961 cggcgtggca cggaaggctg gcctggccca atggcgcgag gccctcgacc gcctgctggg
     1021 cctgcccaac tacgaacgct atctggacca catgcgggaa gcgcatcccg ggctgccggt
     1081 tttgtcgcgc cgggcgttct atgatgaggc ccagaaatcc cggtacgaga gtggcagcga
     1141 ccggccacgt tgttgctaaa gccagtacct ggacaggttg acccaggcgt ccaaaacctg
     1201 ggtcaaccgg ccacgggcct atattggaca ttaacaaaag tccgattatg ctttgtgtag
     1261 gctaaatccg ctaaaaatca aataccttta cttgttttct ttttgccgcg catgcggcca
     1321 tttctctgag gtcttccgcg atgtgcgatc gcaaattccc acctgctgtt cccgttacgt
     1381 tcctccccgc gcagaggggg ccgaactgac atgatggaga acagccgttt cgtcaaaacc
     1441 tgtgaacatc tggtcttcac ctaccgcgcc tggtacgtgg tggccttttt cctgctgacc
     1501 attttcttcg gctattttgc cgtccacctg aagatggaca gcagcctgga aaaaatggtg
     1561 cccatgcatc acgagtacat ccagaactgg ttcaagcaca aggatgatct cagccttggc
     1621 aatgatttgc gcatcgcagt ggaagtcacc gaaggcgaca tcttcaatgc cgagttcctg
     1681 gaagtgctgc gtctggtaac ggacgaggcc ttctatatcg aaggtgtgga caagtccaag
     1741 atccagtcac tgtggacacc ggatgtacgc tggatcgagg tgaccgaaga aggtttccgt
     1801 ggcggtgagg tgattccgcc ggattatgac ggctcggaag catcgctcaa ggtggtgcag
     1861 accaacgtgc tgcgttccgg tcaggtcggg cggctggtgg gtgacaattt caagtcggcc
     1921 attgtgtatg tgccgctgct gttcctggat gccagcaagg ggcagaaaca cgattaccgc
     1981 aagctgtccc acgcgctgga agaaaaaatc cgcgaccgct tccagaacga caagatcaag
     2041 attcatatcg tcggcttcgg caagaagatc ggtgacatga tggatggtgc catcgaggtg
     2101 gccaccttct tcatcatcgc gctggtgctc acgttcctgc tgctgctgat ggatacccgc
     2161 tgcctgcgca gttccctggt ggtgacgctt acgtcagtca gtgcggtgat ctggcagatg
     2221 ggtatcatca ccctgctggg ctatgggctc gatccctatt ccatgctggt gccgttcctg
     2281 atttttgcca tcgcggtgag ccacagcgtg cagatcgtgc agagttttgc ggtggcgcgg
     2341 gcggaaggac aggagaacct gccggcggcg cgcactgtgt tccgctccct gttcgcggca
     2401 ggcgcttcgg cgttgctgtg cgatgccatc ggtttctact cgctgatcat catcgacatt
     2461 cgcgtgatca aggaactggc catcgcggcc ggtgccggga cgctggtggt caccttcacc
     2521 aacctgattc tggtgaaagt gctgttcagt tataccggtg tcagccaggc ggccatcaac
     2581 caggtgctgc gcaagcgttc caaaccgcag aaggcctggc tgttcctgtc caagttcgct
     2641 gatccgcgca tcgcggcggt gtccatcctg atagcggcga ttctgggcgg cgggtcgttc
     2701 tacaaggcga aggatctgaa gaccggtgac ctcgatccgg gcgcgccgga attgtggccg
     2761 gattcccgct acaaccgcga cgatgcgttc gttacctcga attattctgt cagcgccgac
     2821 gtgctggtgg taatggtgga aacccagccg gacaagtgca ccagctatcc ggtggtggaa
     2881 gccatggacc gcttcatgtg gcatatggaa aacgtgcccg gcgtgcagtc ggcagtgtcg
     2941 ctggtgacgg tgtccaagcg catgctcgcc ggcgtcaacg aaggcaacct gaagtggcag
     3001 cagctcacca aggtggagga aattctcaac aactcgatgc cgtcagacat ggtgtccagc
     3061 ctgatgaacg tcagctgcag cctggcgccg gtgatcgtgt tcctgcagga tcacaaggcg
     3121 gaaacgctca aggccgtcac cagggctgcg gaggattttg ccagggagaa tgacaatccg
     3181 gaggtcgccc ggttcgtgct ggcgacaggt aacgccggcg tcgaggccgc caccaaccag
     3241 accgtggaac gcgctgccag caccatgatg tggctggtgt acggcatcgt gatcatcatg
     3301 tgctggctga cattccgctt ctccatcatc gccgtgatct gcgtgatcac gccgctggtg
     3361 ctgacgtcgc tgctgtgcga agcgctgatg gcggttttcg gcatggggtt gaaagtggga
     3421 acgctgccgg taattgcgct gggtgtggga atcggtgtgg attacggtat ctacatctac
     3481 tcgaagatga tggagttcat ggccaagggc caggatctgc gcgaagccta tttcaacacg
     3541 ctgtgtgtga cgggcaaggc catcacgttc acggcagtat cgctggcact gtgcgtgggt
     3601 acctgggcgt tctccgacat caagttccag gctgacatgg gcatcctgct gaccttcatg
     3661 ttcgtctgga acatggtggg tgcgatgtgg ctgctgccgg cgatggcgga cttcctgttc
     3721 aagttccaga agaagaaggc tgtcgccaag gcataatgcg ggcgggacca aaatgaatgc
     3781 ccggcgttga gtcgatcaac gccgggcatt tttatttcgg ggatagattt tgagaatcag
     3841 gtattgagag tcagacaacg taaatcccga ctcagtggca gggtaccggg ctcatttgtg
     3901 ttggattgtt ttttaaacag gagcagtgct taaacgaatc gtggtcaggt atcggaaacg
     3961 ccggtagcaa ggcgatctgg caacactacc tattggcggc ctgtatttat acttgcttac
     4021 tgtaagtcga ttttgtttag aatcgggctg caggcaagca aaatgacctg acatggattg
     4081 caaaaatgaa tatggatttg tttgaggtct tcagcatatg acgcaagatt cccgcctgat
     4141 caggctgacg gccaacttta ttgatgattc ctgtgtggca gtccttctgg tcggccggga
     4201 gtcgttgtcc caaccgtatc agtttcagat cgaattccag tctaccatca gtacgttgct
     4261 gagccaggat gtaattggcc agaaagtgtc tgtggcaatt cacccgcagg ggattctgca
     4321 gccccatttc ctgcatggtc gtgtgaatca gttgcaattg caggatgtca cggacaacgg
     4381 cacccgtgtg taccagattt cattggtgcc tggattgtgg tttttgtctc aggccggtac
     4441 caatcgcatt tttgaaaaca agaccagtat tgatatcgcg caggagatct tcaaggcc
//
LOCUS       DASUEG010000768         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1689718, whole genome shotgun sequence.
ACCESSION   DASUEG010000768 DASUEG010000000
VERSION     DASUEG010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689718"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2879)
                     /gene="putA"
                     /locus_tag="VFX11_14525"
     CDS             complement(<1..2879)
                     /gene="putA"
                     /locus_tag="VFX11_14525"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX4939890.1"
                     /translation="MAAPRCKAPTSLGLEFPLSDDILGSFLRPEEELVQELLLGYTLD
                     AKTREQTRAYARDLLNAIRNAKPGLRIETLLKEYGLDSKEGVILMCLAEALLRIPDQE
                     TAERFLQDRLEQGNWAEHLGHSDSLWVNASTWGLLFTGKLMHNADQPAKSLKESLRQL
                     LRRLGEPLAMASIRQAMNVIGHQFVAGETIPDAVSRSRAETEKGYFHSFDMLGESAMT
                     LQDAERYTESYRRAILDIHSQYQGRLAAGSLSIKLSALHPRFELRQGKALDTLHATLL
                     DLLLLAQEKDVAVTIDAEESWRLEPGLMAFARVASHNKIRHWGKLGLAVQAYQKRAPK
                     VLQWLMDVSKTLHCQIPVRLVKGAYWDTEIKQTQQQGLDEYPVYTLKSHSDLSYLYCA
                     QMLLEGKHFLFPQFATHNAHTVASIITLAEEKGLKQFEFQRLHGMGEALYDHVLSTFE
                     DAQCRVYAPVGNFQDLLPYLVRRLLENGANTSFVRQIQLAQSDGTWLIEEPSDAIKAL
                     DRFRNPKIPLPRRVFYPERRNSRGLNLEALESLHQLSKAMKPLLNQRKDLQPDITDAE
                     RRREIRSPANPNEIVGSFVPASRQDCLQAFDRSREAWQQWRFSEVDERATLLEETALQ
                     FEKHYEELMLLAMREAGKTLNNALAEVREAVDFLRYYANQARKLFDPQELPSVTGEDN
                     KLLLEGKGLVLCISPWNFPLAIFTGQVAAALAAGNCVIAKPSTLSPLIAARAIELFHQ
                     AGFPDDVVQLVTANAKTIEDSILQEAALRAVMFTGSTSAATHINRQIANRNGPIISLI
                     AETGGQNAMIVDSSALVEQVTRDIAQSAFDSAGQRCSALRILFVQEDVKERVLNTLSG
                     YLDTFKIGDPLDWETDIGPVINADARSELLLHIERFRSRNRVRYQAPLPELPGHYVPP
                     TIIELDDINELHEEIFGPILHVVSFKADRFDDVIEQINRTGFGLTL"
     gene            complement(3023..>3708)
                     /locus_tag="VFX11_14530"
     CDS             complement(3023..>3708)
                     /locus_tag="VFX11_14530"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939891.1"
                     /translation="AGDYIEIIDIRDPESPTHKGELQTPGQGKDVALVGNLAFIAHGY
                     EGLRVVNIADPANPSPPVDLDTPGAAHGIDVAGDYAYIADSEYGLQAIKFSQTDELKV
                     ELGPAVRVDTPGSASEVVVSGNIAYVADSQAGLQLIDISNPDKPRLIGGVDTPGNAID
                     VTIMNSIAYVADDFAGVQVIDVRDPYNPTLVGAARTNSGAMGISAAGDHIYVATHFGL
                     EILHAIPTE"
BASE COUNT          673 a         1077 c         1161 g          797 t
ORIGIN      
        1 agggtgaggc cgaagccggt gcggttgatc tgctcgatca cgtcgtcgaa gcgatcggcc
       61 ttgaagctga ccacgtgcag gatggggccg aaaatctctt cgtgcagttc gttgatgtca
      121 tccagctcga tgatggtggg cggcacgtaa tggcccggca gttccggcaa cggcgcctgg
      181 taacgcacgc gattccggct gcggaagcgt tcgatgtgca gcagcagttc ggagcgcgca
      241 tcggcattga tgaccggccc gatatcggtt tcccaatcca gcggatcgcc gatcttgaat
      301 gtgtcaaggt aaccggacag cgtattcagc acgcgttcct tcacatcttc ctgcacgaac
      361 agaatacgca gcgcggaaca gcgctggcca gcactgtcga aagccgattg tgcgatgtcg
      421 cgcgtcacct gttccaccag tgcagagctg tcgacgatca tcgcattctg gccgccggtt
      481 tccgcgatca gcgaaatgat cgggccattg cggttggcaa tctgacgatt gatgtgcgta
      541 gcggcagagg tggaaccggt gaacatcacc gcccgtagcg ccgcttcctg cagaatgctg
      601 tcttcaatgg ttttggcatt ggcggtgacc agttgcacca catcgtcggg aaaaccggcc
      661 tgatgaaaca gttcgatggc gcgggcggcg atcagcggcg acagcgtgga cggcttggcg
      721 ataacgcagt tgcccgccgc cagtgccgcc gccacctggc cggtgaaaat cgccagcgga
      781 aaattccacg gactgatgca cagcaccagg cccttgcctt ccagcagcag cttgttgtcc
      841 tcgccggtga cgctgggtaa ttcctgcgga tcgaacagct tgcgggcctg gttggcgtaa
      901 tagcgcagga aatccactgc ctctcgcact tccgccagcg cgttgttcag cgttttgccc
      961 gcttcacgca tcgccagcag catcagttct tcataatgtt tttcaaattg cagcgcagtt
     1021 tcttccagca gggtggcgcg ttcatccact tcagaaaaac gccactgctg ccaggcctcg
     1081 cgactgcggt cgaacgcctg caggcagtcc tgccggctgg cgggaacgaa gctgccgacg
     1141 atctcgttgg gattggcggg cgagcggatc tcgcggcggc gctcggcgtc ggtgatgtcc
     1201 ggctgcagat ccttgcgctg attcagcagt ggtttcatcg ccttgctgag ttgatgcaat
     1261 gattccagcg cttccagatt cagcccgcgc gaattgcgtc gttcggggta gaacacccgc
     1321 cgtggcagcg gaattttcgg attgcgaaaa cggtccagcg ccttgatcgc atcgctgggt
     1381 tcctcgatca gccaggtgcc gtcgctctgg gccagctgga tttgccggac gaaagacgtg
     1441 ttggcgccgt tttccagcag ccgtcgcacc agatagggca gcagatcctg gaaattgccc
     1501 accggcgcat aaacgcgaca ctgcgcatct tcgaacgtgg aaaggacatg gtcgtacagc
     1561 gcctcgccca tgccgtgcag gcgctggaat tcaaattgtt tgaggccctt ttcttccgcc
     1621 agcgtgatga tgctggccac cgtgtgcgcg ttgtgggtgg caaattgcgg gaacaggaaa
     1681 tgcttgcctt ccagcagcat ttgcgcgcaa tacagatagc tgagatcgct gtgggatttc
     1741 agcgtgtaca ccgggtattc gtcgaggccc tgctgctggg tctgtttgat ttcggtatcc
     1801 cagtaggcgc ctttcaccag ccgcaccggg atctggcagt gcagagtctt gctcacatcc
     1861 atcagccatt gcagcacttt aggcgcgcgc ttctggtacg cctgcacggc caaccccagc
     1921 ttgccccagt gacggatctt gttgtggctg gcgacgcggg cgaatgccat caggcccggc
     1981 tccaggcgcc aggattcttc cgcgtcgatg gtcaccgcca catctttttc ctgggccagc
     2041 agcagcaggt ccaacagggt ggcgtgcaac gtgtccagtg ccttgccctg ccgcagctca
     2101 aagcgcgggt gcagcgccga cagcttgatc gacaggctgc ccgctgccag acggccctga
     2161 tactgggaat gaatgtcgag aatcgcgcgg cggtaagact cggtgtagcg ctcggcgtcc
     2221 tgcagggtca tcgccgactc gcccagcatg tcgaaagaat ggaagtagcc tttttcggtt
     2281 tcggcgcggc ttcggctgac cgcatccgga atggtttccc ccgccacgaa ctggtggccg
     2341 atcacattca tggcctgacg aatggacgcc atcgccagcg gctcccccag ccggcgcagc
     2401 aactggcgca gggattcttt cagcgacttg gccggctgat cggcattgtg catcagcttg
     2461 ccggtgaaca gcaggcccca ggtggacgca ttcacccaca gcgagtcgct gtggcccaga
     2521 tgctcggccc agttaccctg ttccagccga tcctgcagga agcgctcggc ggtctcctgg
     2581 tccgggatgc gcagcagcgc ctccgccagg cacatcagga tcaccccttc cttgctgtcg
     2641 aggccgtatt ccttcagcag cgtctcgatg cgcaggccgg gcttggcatt gcggatggcg
     2701 ttgagcaggt cccgggcata ggcacgggtc tgctcgcggg ttttggcgtc cagggtgtag
     2761 ccgagcagca gctcctgcac caattcttct tcgggtcgca ggaaactgcc cagaatgtcg
     2821 tcgctcaggg ggaattcgag gcccagggag gtgggggctt tacagcgggg ggctgccatg
     2881 gactacttcc tgtctctatg ccgtatcaaa cacttagtag gttaagtgtg gcacagggcg
     2941 gcgggggtgt caggggctgg ttacatggtt cgcaggcact gctgctgttg gccagtgacg
     3001 gtgcctgcga acggggtagg gcttattcgg tgggaatggc gtggaggatt tccagtccga
     3061 agtgggtggc aacgtaaata tgatctcccg cagcgctgat gcccatggcg cctgagttgg
     3121 tacgagctgc gccaacgagt gtgggattat agggatcgcg gacatcgatg acctggacgc
     3181 cagcgaagtc atcggcaacg taggcgatgg aattcatgat ggtcacatcg attgcattgc
     3241 ccggggtgtc caccccgcca atgagtctgg gcttgtcagg attgctgata tcgatcagtt
     3301 gcaagccagc ctggctgtct gcaacgtagg cgatgttgcc tgacacaacc acttcgctgg
     3361 ccgatccagg tgtgtcgacg cggaccgcgg gtcctagctc taccttcagt tcgtccgtct
     3421 gactgaattt gattgcctgc agcccgtatt cgctgtccgc gatgtaggca taatcacccg
     3481 ctacgtcgat accgtgtgct gcgccgggtg tgtccagatc gaccgggggt gatgggtttg
     3541 cgggatcggc aatgttcacg actcgcaaac cttcatagcc atgggctata aaagccagat
     3601 tgcccaccag cgcgacatct ttgccttgtc ctggggtttg gagctcgcct ttgtgggtcg
     3661 gcgattccgg gtcacgaata tcaatgatct cgatatagtc accggcgg
//
LOCUS       DASUEG010000769         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1644113, whole genome shotgun sequence.
ACCESSION   DASUEG010000769 DASUEG010000000
VERSION     DASUEG010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644113"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="gspM"
                     /locus_tag="VFX11_14535"
     CDS             complement(<1..383)
                     /gene="gspM"
                     /locus_tag="VFX11_14535"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX4939892.1"
                     /translation="MSANAKEQLQNYVDMARRRYDSLTDTERLVVNLVGLLVLAALVF
                     LLLVAPAQRSVSEAQLKVSGQQKLVQWMKENESLARMAASAGTAAPKSDQPLQTIVTS
                     TAGPLGITVKRYENESDTKLRVWLE"
     gene            complement(380..1735)
                     /gene="gspL"
                     /locus_tag="VFX11_14540"
     CDS             complement(380..1735)
                     /gene="gspL"
                     /locus_tag="VFX11_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX4939893.1"
                     /translation="MTTKVYVRFINENQWISLCDSVLDDSPIDMLDDLLVEWAWSDAE
                     QPVSDSQVVPFSQFVTDVKQRWPHSYQYGIDLILSGAYAVGASVNIPSKQMRHIAQAL
                     PYMLEDQLAQEVGHFHLIHGSRSEEGALPVLAVPSRILAVTRRLFNEQDLPLDAVMAD
                     MLCLPHKAGEWTVLADGKHLLLKQGDLAGISIEIDAAPVVLASIFQYWQPRPSVLRVL
                     LCQEHLNDNLKNWVKTQINSALADQDIDVEYDEIASNYFMVLCDHLQHVNRKNPANFL
                     QGPFAASGRRKPSTYNWKPLAALVATFVILHTVFLYTQSWRLNSEVRRLDTEATTLYK
                     RLFPGDRKVVNVKRQMEQHIQNFQKGQGGDGFLALLAKTGEQIRSMNSTQKGSITPQR
                     AAFDDNQGDLRLDLMAKDFAQLENLKNRLEAADLAVETASAAQDAGVVKARLKIRSQG
                     T"
     gene            complement(1772..>2098)
                     /locus_tag="VFX11_14545"
     CDS             complement(1772..>2098)
                     /locus_tag="VFX11_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939894.1"
                     /translation="DYSDLNQIMSILNPGGTAPQQVPQQPQTPSAIPGAPVSPSAPGN
                     PLQGFGVTSNYFLLKANALINGKTVKMNSIIWRIDAKDFASPQPGQPKVTPIKTVLRK
                     LVDPLE"
BASE COUNT          417 a          607 c          595 g          479 t
ORIGIN      
        1 ttttccagcc acacccgcag tttggtgtcc gattcatttt cataccgctt cacggtgatg
       61 cccagcggtc cggcggtgga ggtgacgatg gtctgcagcg gctgatcact tttgggagcg
      121 gccgtgccgg cactcgctgc catgcgtgcc agcgattcgt tttccttcat ccactgcacc
      181 agcttctgct ggccactcac tttcagttgc gcctcgctca cggaacgctg ggcaggcgcc
      241 accagcaaca ggaaaaccaa cgccgccagt accagcaagc ccacgagatt aaccaccagg
      301 cgctcggtat ccgtcagact gtcgtagcga cggcgggcca tgtcgacata attctgcaac
      361 tgttcttttg cgttggcgct catgtgcctt gactccgaat tttcaggcgg gccttgacca
      421 ccccggcatc ctgggcggca gacgcggttt ccaccgccag gtccgcagct tccaggcggt
      481 ttttcagatt ttccagctgg gcaaaatcct tcgccatcag gtccagccgc aaatcaccct
      541 gattgtcgtc gaacgctgca cgctgcgggg tgatggagcc cttctgcgtg gaattcatgg
      601 agcggatctg ttcaccggtt tttgccagca gtgcaaggaa accatcgcct ccctgtcctt
      661 tctggaaatt ctggatgtgc tgttccatct gccgcttcac gttcaccact ttgcggtcac
      721 cggggaatag ccgcttgtag agggtggtcg cttcagtgtc cagtcgcctg acctcggaat
      781 tcaaccgcca cgactgtgtg tagaggaaca cggtatgcag gatcacaaac gttgccacca
      841 gcgccgcgag cggtttccag ttgtacgtcg acggtttgcg ccggccggat gcagcaaacg
      901 gaccctgcag gaagttcgcg ggattcttgc ggttcacatg ctgcagatga tcgcacagca
      961 ccatgaagta attcgacgcg atctcgtcat attcaacgtc aatgtcctgg tctgccaggg
     1021 cgctgttgat ctgggttttg acccagttct tcaggttgtc gttcaggtgt tcctggcaca
     1081 gcaagacccg cagtacgctg ggcctgggct gccagtactg gaaaatcgac gccagcacca
     1141 ccggcgctgc atcgatctcg atggatatac cggccagatc cccctgcttg agcagcaggt
     1201 gcttgccgtc cgccagcacc gtccactcac cggccttgtg gggaaggcac agcatgtcgg
     1261 ccattacggc atccagtggc agatcctgct cattgaacag gcgacgggtg acggccagaa
     1321 tccgggacgg gacagccagt accggcaaag cgccttcctc gctgcggctg ccgtggatca
     1381 aatgaaaatg gccgacttcc tgcgccagct ggtcctccag catgtaaggg agtgcctggg
     1441 cgatgtgccg catctgcttg gacggaatat tgaccgatgc accaacggcg taggcgcccg
     1501 aaaggatcag gtcgatcccg tactgatagc tgtgaggcca gcgctgtttg acgtcggtga
     1561 caaactgact gaagggtacc acctgggaat cagagaccgg ctgctcggca tcggaccagg
     1621 cccattccac cagcaggtcg tcaagcatgt cgatcggact gtcatccagc accgaatcgc
     1681 acagactaat ccactgattt tcattgataa agcgcacgta aactttggta gtcacaggac
     1741 atgccctaac gttcttttgt tttaattata ctcattccag cgggtctacg agctttctca
     1801 gcacggtttt tatcggggtg accttgggct ggcctggctg cggagatgca aagtccttgg
     1861 catctatccg ccagatgatc gaattcattt tgactgtttt accattaatg agggcatttg
     1921 ccttcaagag aaagtaattg ctggtgacac cgaagccctg gagcgggttt ccaggcgctg
     1981 acggagacac gggggcgcca ggaatagctg agggtgtctg tggttgctgc ggaacctgct
     2041 gcggtgcggt ccctccgggg ttcaggatcg acataatctg atttaaatcc gagtaatc
//
LOCUS       DASUEG010000770         5364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1353039, whole genome shotgun sequence.
ACCESSION   DASUEG010000770 DASUEG010000000
VERSION     DASUEG010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353039"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFX11_14550"
     CDS             <1..834
                     /locus_tag="VFX11_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX4939895.1"
                     /translation="VMIPGVNDEHLKEVSRIVKQKGAFLHNVMPLIAEAEHGTFYGVM
                     GQRSPEPEELQDLQDSCSGDMNMMRHCRQCRADAVGLLGEDRGEEFTMDKIEEMEIDY
                     AAAMEKRAVIHAAIMEELDEKQAKKSRLAAMSVPTTETAVSKHYRPVLMAVATSGGGI
                     INQHFGHAKEFLVYEASPTGVRFISHRKVDQYCIGNDACGEKESALTGAIRALKGCEA
                     VLCSKIGYEPWSSLEEAGIHPNGEHAMEPIEDAVMAVYQEMAAAGKLEAAAPERLEVT
                     A"
     gene            868..1146
                     /locus_tag="VFX11_14555"
     CDS             868..1146
                     /locus_tag="VFX11_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX4939896.1"
                     /translation="MALKIVELCVNCWACVDVCPSDAIYHSSPHFLINPSKCTECEGD
                     HADPQCASICPIESAILNAAGEAINPPGSLTGIPPEKMEEAMREIQAR"
     gene            1175..1636
                     /locus_tag="VFX11_14560"
     CDS             1175..1636
                     /locus_tag="VFX11_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939897.1"
                     /translation="MVTVLFYEKPGCASNARQKQILRQAGATLSVRNLLTEAWTAPRL
                     RTFFGTRPVPEWFNPSAPAIKSGHVDPLNIGADSALAAMLVDPLLIRRPLIEADEFRC
                     SGFDWPALAHALGLQAIPDPLTTTESGSKNLESCSRDSSAGRCSTITGGQE"
     gene            1633..2220
                     /locus_tag="VFX11_14565"
     CDS             1633..2220
                     /locus_tag="VFX11_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HEX4939898.1"
                     /translation="MNAQLALLPDVNAAQNSNHEWLSLILLAQRNGEGCLPYNLGLDF
                     SGYTQLLQRLAVPFQTEQCEVEITAANQALEKGALRQQLLEMRRDEWQEIRALLLNGR
                     RNADIAEIWLADIVAAGCLGGNHLWRDLGLPSRAALRDMLLHNFPVVALRNDRDMRWK
                     KFFYKQLCEQQGGYVCRSPSCDQCPTYQDCFGDEQ"
     gene            2217..2621
                     /locus_tag="VFX11_14570"
     CDS             2217..2621
                     /locus_tag="VFX11_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4939899.1"
                     /translation="MSKFNRISVDEAEWLLLQHENLLLLDMRDMNAYCRHHHPRALHL
                     NDMNMRSLLKHSPKNIHVVIYCYRGNSSQDMARLFADFGFENCYSVDGGFDAWRLALV
                     QSVTLPGESLQRWIAARDLPSSPKPVRAEARA"
     gene            2663..3013
                     /gene="grxD"
                     /locus_tag="VFX11_14575"
     CDS             2663..3013
                     /gene="grxD"
                     /locus_tag="VFX11_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX4939900.1"
                     /translation="MTTSNTEAVIRQQIATNPVLLYMKGTPDAPECGFSGATVAALKR
                     SAVPFSFVNVLASPFIRERLPSISRWPTFPQLFVDGELIGGCDIVLGMEADGSLKPLL
                     DGAGKSAAVAKAAE"
     gene            3013..3273
                     /locus_tag="VFX11_14580"
     CDS             3013..3273
                     /locus_tag="VFX11_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX4939901.1"
                     /translation="MERQTIVDILQAGVPNAEVLLDGADCSFTVTVVSPHFQGMRPVA
                     RQQLALEGFTEALRTGEIHALSIRAFTPDEWQQQSRPVMPAL"
     gene            3465..3593
                     /gene="ccoM"
                     /locus_tag="VFX11_14585"
     CDS             3465..3593
                     /gene="ccoM"
                     /locus_tag="VFX11_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit CcoM"
                     /protein_id="HEX4939902.1"
                     /translation="MFLDDVVIAGLITVGGTLVFLLGIGVFVYQDAHRKKQEKQHS"
     gene            complement(3617..4351)
                     /locus_tag="VFX11_14590"
     CDS             complement(3617..4351)
                     /locus_tag="VFX11_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4939903.1"
                     /translation="MNSEPCNVAEHRVNVKRSRPADCLRCSLRKKMLFAELELPPIAE
                     LLGAVSTQQIRRGSTLYVQGESGIMLYAVRKGLVKLTQSGADGYQRIVHIAGPGTCVG
                     MEGFLEPGYHQTAEALTDIDVCAIPVLVAGRIATQQPAFYRALLQRWQQQFATAERWM
                     MELSQGTVRQKLARLILMLDDIGDTDGQIRLLSNLDSAGILSTTMETVSRHVAEFKRA
                     GTLAKVGPALYRIDKSGLQQAAESPE"
     gene            4605..>5364
                     /locus_tag="VFX11_14595"
     CDS             4605..>5364
                     /locus_tag="VFX11_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939904.1"
                     /translation="MPTLLVLNCGSSSIKFALYPCAGDRQVASTPLCEGTASRLQEAG
                     TGQLRISRNGEPQQLALAGDGHAGALQAIIRQLDQWHLLEDISAVGHRVVHGGDRFRQ
                     PTLIDSDVIAQIERYSDYAPLHNPVNLEGIRIARQLLPALPQVAVFDTAFHGTLPPVA
                     YHYAVPAQWHRQWGVRRYGFHGTSHQFIAESIPALTGTAPDTVRAVSAHLGNGCSLCA
                     IKGQHSIDTSMGFTPLEGLVMGSRCGDLDPGLHEY"
BASE COUNT         1139 a         1674 c         1565 g          986 t
ORIGIN      
        1 gtcatgattc ccggcgtcaa tgacgagcac ctgaaggaag tgagccgcat cgtcaaacag
       61 aaaggcgcgt tcctgcacaa cgtgatgccg ctgatcgccg aagccgaaca cggcaccttc
      121 tacggtgtga tggggcagcg cagccccgag ccggaagagc tgcaggatct gcaggattcc
      181 tgttccggcg acatgaacat gatgcggcac tgccgtcagt gtcgcgccga tgcagtgggc
      241 ctgctgggtg aggatcgcgg tgaagagttc accatggaca agatcgagga aatggaaatc
      301 gattatgccg ctgccatgga aaaacgcgcg gtgatccacg cagccatcat ggaagaactc
      361 gacgagaagc aggcgaagaa atcccgcctc gccgccatga gcgtgcccac cacggaaacc
      421 gccgtgagca agcattaccg tccggtactg atggcggttg ccaccagcgg tggcggaatc
      481 atcaaccagc acttcggcca cgccaaggaa tttctggtgt atgaagcctc gcccaccggc
      541 gtgcgtttca tcagtcaccg caaggtggac cagtactgca tcggcaacga cgcctgcggc
      601 gaaaaggaaa gcgcactcac cggcgccatc cgcgcactga aaggttgcga agccgtgctc
      661 tgttccaaga tcggctatga accctggtcg tcgctggaag aggccggcat ccaccccaac
      721 ggcgaacacg cgatggagcc gatcgaggat gcagtaatgg ccgtgtacca ggaaatggcg
      781 gcggccggca agctggaagc cgctgcgccg gagcggctgg aagtcactgc ctgacaacat
      841 tcactgttct gtaatcggga gagggacatg gcactgaaaa ttgtcgaact atgtgtgaac
      901 tgctgggcct gcgtcgacgt ctgccccagc gatgcgatct accacagttc accgcatttc
      961 ctgatcaacc cgtcgaaatg cacggaatgc gaaggggatc atgccgatcc ccaatgcgcc
     1021 agcatttgcc cgatcgaatc ggccattctc aacgccgccg gtgaagccat caatccgccg
     1081 ggatcgctga caggcatccc gccggaaaaa atggaagaag ccatgcgcga gatacaggcg
     1141 cgctgaacgc ttgcggcagg atgaggccat cgccatggtg actgtgctgt tttatgaaaa
     1201 gccgggctgc gccagcaacg cccgccagaa acagatcctg cgtcaggcgg gtgcaaccct
     1261 gagcgtgcgc aacctgctca cggaagcctg gacagcaccg cgcctgcgga cttttttcgg
     1321 tacgcggccg gtgccggaat ggttcaaccc cagcgcgccg gcgatcaagt ctggccacgt
     1381 tgaccccctc aacatcggtg ccgattctgc gctggctgcc atgctggttg atccgttgct
     1441 gattcggcga ccgttgattg aggccgatga attccgttgc agcggtttcg actggccggc
     1501 actggcgcat gcgctcggtc tgcaggcaat acccgacccg ctgacaacaa cggaatccgg
     1561 cagcaaaaat ctggagagct gttcacgcga ttccagtgcc ggccgctgca gcaccataac
     1621 aggaggccaa gaatgaatgc ccaacttgcc ttgctaccgg acgtcaacgc cgcgcagaac
     1681 agcaatcacg aatggttgag cctgatcctg cttgcacaac gcaacggtga aggttgcctg
     1741 ccatacaacc tcgggcttga tttctccgga tacacgcaat tgctgcaaag acttgccgtg
     1801 cccttccaga cagaacagtg cgaagtggaa atcaccgccg ccaaccaggc cctggaaaag
     1861 ggcgcactgc gccagcagct gctggaaatg cggcgcgatg aatggcagga aatccgcgcg
     1921 ctgttgctga acggccgccg caacgccgac atagcggaaa tctggctggc cgatatcgtc
     1981 gctgccggct gcctcggcgg caatcacctg tggcgcgatc tgggcctgcc gtcgcgggcc
     2041 gcgctacggg atatgctgct gcacaatttt cccgtcgtgg ccctgcgcaa cgatcgcgac
     2101 atgcgctgga agaaattctt ctacaagcaa ctctgcgaac agcagggcgg ttatgtctgc
     2161 cgctctccca gctgcgacca gtgccccacc taccaggact gttttggaga cgagcaatga
     2221 gcaagttcaa tcgaatcagt gtggacgaag ccgaatggtt gttgctgcaa cacgaaaacc
     2281 tgctgctgct cgacatgcgc gacatgaacg cgtactgccg ccaccatcat ccccgcgccc
     2341 tgcatctcaa cgacatgaac atgcggtcgc tgctgaagca ctcacccaaa aatattcacg
     2401 tcgttattta ctgctaccgc ggcaattcca gccaggacat ggcacgcctg ttcgccgatt
     2461 tcggttttga aaactgttac agcgtggatg gcggtttcga tgcctggcgt ctggcgctgg
     2521 tgcaatccgt cacgctgccg ggtgaatcgc tgcagcgctg gattgcggcc cgcgatctgc
     2581 catcgtcacc aaagccggta cgggcggaag cgcgcgcctg aacacatcaa ttgtcttcaa
     2641 cctgagaatc aaggacacca ccatgactac ctccaacact gaagccgtca tccgccagca
     2701 gatcgccacc aatccggtgc tgctgtacat gaaaggcaca ccggacgcgc cggagtgcgg
     2761 cttttccggc gccactgtgg ccgcgcttaa acgctccgct gtccccttca gcttcgtcaa
     2821 cgtgctggcg tcaccgttca ttcgcgagcg gctgccttcc atatcgcgct ggcccacctt
     2881 cccgcagctg ttcgtggacg gcgagctgat cggcggctgc gacatcgtgc tgggcatgga
     2941 agcggacggt tccctcaagc cgctgctgga tggtgcgggc aaatcggcag cggttgccaa
     3001 ggcggccgag tgatggaacg gcagacgatt gtcgacatcc tgcaggctgg tgtacccaat
     3061 gcggaagtgt tactggacgg tgccgactgc agctttaccg tgaccgtggt cagtccgcac
     3121 ttccagggca tgcgaccggt ggcgcggcag caactggcgc tggaaggttt caccgaggca
     3181 ctgcgcaccg gcgagataca cgccctgagt atccgcgcct tcacgccaga cgaatggcag
     3241 cagcaatcca ggccagtaat gccggcgtta taacaggcgc cggcactata accagcgtct
     3301 gcgccacgac gaaaacccgg cacctgcgca tctgttgacc cgcatcaaag cgcgctgcac
     3361 tttccagcgg cttgtgcctg cgcgccccgg aaaaacctga ccgacgtcat gggaatactg
     3421 caccccgctg ctagcctgaa agcagttgat agcggaggtg tatcatgttc ctagatgatg
     3481 ttgttattgc aggtttgatt actgtcggtg gcacgctggt gtttctgttg ggaatcggag
     3541 tattcgtgta ccaggatgcc catcgcaaga agcaggaaaa acagcatagt tgaaggatca
     3601 gtacaagcgg ggcagcttac tcagggctct cagctgcctg ctgcaaacca gacttgtcga
     3661 tccggtacag tgccggccct acttttgcga gggtgccggc acgtttgaat tccgcaacat
     3721 gacggctgac tgtttccatc gtggttgaca gaatgccggc actatccaga ttgctgagca
     3781 gccggatctg tccgtccgtg tcaccgatat cgtccagcat cagtatcagc cgcgccagtt
     3841 tctggcgcac cgttccctgc gataactcca tcatccagcg ttccgccgtg gcaaactgtt
     3901 gttgccagcg ctgcagcaac gcgcggtaaa aggccggctg ctgcgtggca atacgcccgg
     3961 ccaccagcac gggaatcgcg cacacatcta tgtctgtcaa agcttccgcc gtctggtgat
     4021 agccgggttc cagaaatcct tccatgccca cacaggtacc ggggccggcg atatgcacaa
     4081 tgcgctggta gccatcggcg ccggactgcg tcagtttcac caaccccttg cgcaccgcat
     4141 acagcatgat gcctgactca ccctgcacat aaagggttga tccacgccgg atctgctggg
     4201 tactgacggc gcccaacaat tcggcaatcg ggggcagctc cagttcggca aacaacattt
     4261 ttttgcgcag ggagcagcgc aggcaatcgg cagggcgact gcgtttcaca ttcacccggt
     4321 gctctgcaac attgcaaggt tcactattca cgggcacacc tgatgcaatt ctccaaggac
     4381 ggaatcactg gcttgcaaga ataggcccgt gcccgacacg caaccatgcg ctggagcagg
     4441 ttttgctatc ggcagtaaaa aagcctggaa aaaaggccag gaaaaagcca gggaacgccg
     4501 tgccgtggca gcaaagccac ccccgtttgg cgcggggcga ctatgttaga atcccgacag
     4561 gattgcttcc cgagaatgtc ccgaaatcgc caggagcctg cgacatgccc accctactgg
     4621 tgctgaactg cggcagttca tccatcaagt ttgcactcta cccctgcgca ggggaccgcc
     4681 aggttgccag caccccgctg tgcgagggca ccgccagccg gctgcaggaa gccggtaccg
     4741 ggcagctgcg catcagccgc aatggtgaac cgcaacagct ggcactggcg ggcgatggtc
     4801 atgccggcgc gttgcaggcc atcatccgcc agctggatca gtggcacctg ctggaagaca
     4861 tcagcgccgt cggccatcgc gtggtgcacg gcggcgaccg cttccgccag cccacgctga
     4921 tcgacagcga tgtgatcgca cagatcgaac gctattccga ctacgccccg ctgcacaatc
     4981 cggtcaatct ggaaggcatc cgcatcgccc gtcagctgct gccggcttta ccccaggtgg
     5041 cggttttcga cacggccttt cacggcaccc tgcctccggt ggcgtatcac tacgcggtgc
     5101 cggcgcaatg gcaccgccaa tggggcgtgc gccgttacgg ctttcacggc accagccacc
     5161 agttcatcgc cgaatccatt cctgccctca ccggcaccgc gccagacacc gtgcgcgccg
     5221 tctccgcgca tctgggcaac ggctgcagcc tgtgcgcgat caagggccag cacagcatcg
     5281 acacctccat gggcttcacc ccgctggaag gactggtgat gggtagccgt tgcggcgatc
     5341 tcgatccagg cctgcacgaa tacc
//
LOCUS       DASUEG010000771         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1621715, whole genome shotgun sequence.
ACCESSION   DASUEG010000771 DASUEG010000000
VERSION     DASUEG010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621715"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1009
                     /locus_tag="VFX11_14600"
     CDS             80..1009
                     /locus_tag="VFX11_14600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939905.1"
                     /translation="MSIQNKQTVLVIDDEPLNIKVLSQALSPWYRVKAATSGADGIRV
                     ATSDDPPDLILLDIAMPGMDGYEVCDQLKSNHATKQIPVIFITARNSTDDEAGGLERG
                     AVDYITKPFSLPIVMARIRNQLLLKIKTDMLEQLVSIDSLTEVANRRRFDEMLDQEWR
                     RSVRNESPLSIIMIDVDYFKALNDSFGHAAGDVCLKQIALALKNSLRRSSDTLARYGG
                     EEFAAILPNISHADAMKLAEQMRHNVEALGIAHPRSPLGPHVTVSLGLTTAQPKMHES
                     PAQLLVQADEMLYQAKQEGRNRIKGANLAANAS"
     gene            complement(1141..>2124)
                     /gene="recA"
                     /locus_tag="VFX11_14605"
     CDS             complement(1141..>2124)
                     /gene="recA"
                     /locus_tag="VFX11_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX4939906.1"
                     /translation="QFGKGAVMRMGDQQHIAIPAISTGSLGLDIALGIGGLPKGRIVE
                     IFGPESSGKTTLTLSVIAEAQRQGLTCAFVDAEHALDPGYAGKLGVNVDDLLVSQPDT
                     GEQALEITDMLVRSNAVDVIIVDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKIT
                     GSVKQANCLVIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAVKQGEEV
                     VGSETRVKVVKNKVAPPFKQAEFQILYGKGIYRMGEVLDLGVLCGFVEKSGAWYAYKG
                     DKIGQGKANAAKFLEDNPQIAQTIEREIRTHMLSSNLPLPSASEEVELEDA"
BASE COUNT          460 a          681 c          560 g          423 t
ORIGIN      
        1 gctccacccc gctgcaccgc cttgacgcga ccaagttttg tactaaattc attagcagaa
       61 cgtcctgctg tggggtgcca tgtccataca aaacaagcaa accgtactgg tcatcgatga
      121 cgaacccctt aacatcaagg tgctgtccca ggcgctgtcg ccctggtatc gggtcaaggc
      181 agccaccagc ggagcagacg gcatccgggt tgccaccagc gatgacccgc ccgatctgat
      241 cctgctggac atcgccatgc ctggcatgga tggctacgag gtttgcgacc agctcaaatc
      301 caatcacgcc accaagcaga ttcccgtcat tttcatcact gcgcgcaatt ccacagacga
      361 cgaagccggc ggtctggaac ggggcgcggt ggactacatc accaaaccgt tcagccttcc
      421 tattgtcatg gcccgcatcc gcaaccagct gctactgaaa atcaagaccg acatgctgga
      481 acagctggta tccatcgaca gccttaccga ggttgcaaac cgccgccgct tcgatgaaat
      541 gctggatcag gaatggcgcc gcagcgtgcg caacgaatcg ccgttgtcga tcatcatgat
      601 cgacgtggat tacttcaagg cactcaacga cagctttggc catgccgccg gcgacgtctg
      661 tctcaaacag attgctctgg ccctgaaaaa cagcctgcgc cgttcttccg acacccttgc
      721 ccgctacggt ggcgaggaat ttgcggccat tctgcccaac atcagccatg ccgacgccat
      781 gaagctggcc gagcagatgc gtcacaatgt tgaggcattg gggattgcac acccgcgttc
      841 accgctgggc ccccatgtca ccgtcagcct tgggctgacc actgcacaac caaaaatgca
      901 tgaatcacca gcgcagctgc tggtccaggc cgacgagatg ctgtaccagg ccaaacagga
      961 aggacgtaac cggatcaagg gagcgaatct ggccgcaaac gcgtcgtgac ggcacgaata
     1021 cactgagcgg aaaaccgaag tccggccgat aaattgagac aagaccgaaa taaaaagcgg
     1081 gagcctttaa ggggcgtccc gctttttatt tgtacttgat aactggtttc acaccccgaa
     1141 tcaggcgtct tccagctcca cttcttcaga tgcagagggc agcggcagat tcgaactgag
     1201 catgtgggta cggatttccc gttcgatagt ctgcgcaatc tgcggattgt cttccaggaa
     1261 tttggctgcg ttcgccttgc cctgaccgat cttgtcgcct ttgtaggcat accaggcgcc
     1321 ggacttttcc acgaatccgc acagcacacc cagatccagc acttcgccca tgcggtagat
     1381 gcccttgccg tacaggatct ggaattcagc ctgcttgaac ggcggcgcca ctttgttctt
     1441 caccaccttc acgcgggttt cgctgcccac cacttcctcg ccctgcttca ccgcgccggt
     1501 gcggcggata tccagacgca cggaggcata gaacttcagc gcattaccac cggtggtggt
     1561 ttccgggttg ccgaacatca caccgatctt catgcggatc tggttgatga aaatcaccag
     1621 acagttggcc tgcttcacgc tgccggtgat tttacgcagc gcctgcgaca tcagacgggc
     1681 ctgcagaccc acatggctgt cgcccatttc gccctcgatt tccgcgcgcg gtaccagcgc
     1741 cgccaccgag tccacgatga tcacgtccac cgcattggaa cgcaccagca tgtcggtgat
     1801 ttccagcgcc tgttcgccag tatccggctg cgacaccagc agatcgtcca cattcacgcc
     1861 cagcttgccg gcatagcccg ggtccagcgc atgctcagca tccacgaacg cgcaggtcag
     1921 cccctggcgc tgcgcttcgg cgatcacact cagggtcagg gtggttttac cggaggattc
     1981 cggaccaaaa atttccacga tccggccctt gggcaggccg ccgataccca gtgcgatatc
     2041 caggcccagc gagccggtgg aaattgcggg gatcgcaata tgctgctgat cgcccatgcg
     2101 catgaccgcg cccttgccaa actg
//
LOCUS       DASUEG010000772         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2087756, whole genome shotgun sequence.
ACCESSION   DASUEG010000772 DASUEG010000000
VERSION     DASUEG010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087756"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFX11_14610"
     CDS             complement(<1..320)
                     /locus_tag="VFX11_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939907.1"
                     /translation="MNLADELTYRILLGLLGGFYFLLRQFYIRKFRALDKTHTRNEQQ
                     ATLGYKTLYAANFFMLVYVFTPWVDFAHLPLPGFLRWLGFAAIIAAFALYAWTHHTLG
                     ELWSG"
     gene            568..2715
                     /locus_tag="VFX11_14615"
     CDS             568..2715
                     /locus_tag="VFX11_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4939908.1"
                     /translation="MTTRLHTTFCRVCEPSCGLLAEVTGQRVTRLLPDREHPVTRGFA
                     CHKGLNFVDIHHDPDRLNQPLKRVSGRQEPGRFETLSWDSAVGEVAERIRALQQQYGP
                     GAIAGYIGNPTAFNALGSQALEHFFMSLGCFRLFTSGTQDCSNKFAGSEAVFGTSTLH
                     PVPDLEHTHYALIFGENPKVSHMSFVSMANPMEKFRAARKRGAVIKFINPRRIESVTP
                     ATGDLVAIKPDTDLYLLAALLHELDAANLFDENIIAQHGEHIDGLRAFIKPYSAETVA
                     EVTGIAAADIKQMAQDFGRAPAACAHMSTGVNMGRQGTLCYWLLHMLSFVTGNLDRPG
                     GNLYSLGFYPAAKAGRLKTPNIFFNSEFGELRLIRGALPGNLLPDLITTPTNPVKALI
                     VLAGNPLLSVGGESRLREAFGQLELIVVLDLFRNATGEMADYLLPCTDMLERSDLNIC
                     GLGMQQQPFVQFTDAVVPAQFERKEEWWILNRLQQALGLPSLFDQGGAGGLYDRLNRM
                     LRYAELSLDDVRNAPHQTVVLPPIPTGRFYSDWLQTESKKVDCCPPLFDEALQRAHII
                     FAELQREDSQQLKLINLRTNYMHNSWYQNMTALKRPGQQFNPLHVSPQDAQRLQLQDE
                     QAVTVRSPWGEITAQVRIDDSLRQGIAAMSHGWGNQRTPGMAVAHQYPGTNVNELLPT
                     GPGSYEKLSNQAFMTGIPVDVEPVSPAAALTPA"
     gene            complement(2702..5266)
                     /locus_tag="VFX11_14620"
     CDS             complement(2702..5266)
                     /locus_tag="VFX11_14620"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4939909.1"
                     /translation="MSEPVLLSDNRIPPFSLLRKHPSPLQCFDAHPHLILHAPPGTGK
                     RTTLLQWLYRQHQPFGWICWSRVPPTDAEFSTPPSVCRVQDVAELRVAVSAQLEQPAT
                     RCLVVEDRSTDGSCLPWRELISAIGARHSTIRLFLLTSRLPAAVSTTLPWSAHVLHFD
                     TLTFSTQEVESVAQLVCAGKVPARWCGALYTATLGWPAALFQLLRNLPALAAPACSEL
                     GCMQVSRWLRLQDFPRPASLLELLALPQKPGALPDAIFAAGAPLAHLHNSLALARQWG
                     YVHCVRDSDNRIRVHGTGFARLTLTHFPAPPPTSATLIACAEWLMQQRYSQAAIDMLV
                     QATQWQRVEDWLLQEGDVLIAQLQVNAVAQWIQTLDLARPIRNPLLLLKAIRCAVYRG
                     DLPAVEQFFSRILILLETQDSAVLQASMPEAQWERLVAEVALYSRMLNRGDAICRRLM
                     DLTTEVWRGADPWTLLREAYQAADAGQILRALPMLSTGLDKAESLDSPSLYLVFANQY
                     CWAQVLSGEMDLAERFLQQVKQWLMHKRIVFMGAYDWLDLLELLLARVRGELADLAPR
                     VAQLLQKPEFTQDFLKHYLLINLQAELALTRGELYVARQAISKLGLLKQGCSARSYWF
                     PSVEVLHRALRRVDEGDDLDLTPLLPHTEMQAADTGSLQQQTGCLWDIKMRLHRRQYT
                     GVLASLEMLAVLCGQSGQWLRLYEIDVLRAVYFYRNGDKPRGETLFRTAMQQIESHRL
                     AGILLDPFLLWQDFVRGETDLPGRDRLIRLYQQARPLEDFNPDRLQQAGLRVPLSRRE
                     RDVLALLVQGLGNQDIADTLHVSITTVRTHVRNLYRKLDVYNRTGATRKALQFKLV"
     gene            5785..>6163
                     /locus_tag="VFX11_14625"
     CDS             5785..>6163
                     /locus_tag="VFX11_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX4939910.1"
                     /translation="MKKSFKRFFALTALSSAILVSGCAQPDSDEPIVRWWDWDPFTGM
                     AYLAEQVLTLYNNNFIDTNVEYPANIDCGATTLAVRTADGTCNDIEQNRMGSTGIRFG
                     RNVQLDAAYPDQANLLEPNPRTIS"
BASE COUNT         1262 a         1875 c         1880 g         1146 t
ORIGIN      
        1 cccgaccaca gttcgcccag cgtgtggtgg gtccaggcgt acagtgcaaa cgcagcgata
       61 atggcagcga atcccagcca gcgcaggaag cctggcaatg gcaggtgcgc gaaatcaacc
      121 cagggtgtga agacatacac cagcatgaag aagttggcgg cgtagagggt cttgtaaccc
      181 agggttgcct gctgttcgtt gcgggtgtgg gtcttgtcga gcgcgcgaaa tttgcggatg
      241 taaaattgac gcagcaaaaa ataaaacccg cccagcaaac ccagcaggat gcgatacgtg
      301 agttcgtcgg ccagattcat ggttcctccc tgactgcggt tgatgactga gatttgggtc
      361 catggtcact gaagattgtc ccggcgtcca tgccagccat tgccaccaca acgatgtctg
      421 ccgacggatg cagtgggtag ccgggtgttg ggcagtcatg atacaacgta ttttatttgt
      481 atcgcttcga taacttacct tgccgattgg ccggcctgtc gttcctgcgc cggcacactg
      541 ggataaattg cagaggaaac ctccatcatg accacccgcc ttcacaccac tttttgccgc
      601 gtatgcgagc cgtcctgcgg cctgttggcg gaagtgaccg gccagcgtgt cacccgtctg
      661 ttgccggaca gggagcaccc ggttacgcgg ggcttcgcgt gccacaaggg gctcaacttc
      721 gtcgacattc accatgatcc ggaccggctc aaccagccgc tgaagcgggt gtcggggcgc
      781 caggagccgg gccgtttcga aaccctcagc tgggacagcg cggtcggcga agtggcggag
      841 cgtatccgcg cgctgcaaca gcagtatggc cccggtgcca tcgccggcta catcggcaat
      901 cccaccgcgt tcaatgccct gggttcccag gcgctggaac atttcttcat gagcctgggc
      961 tgcttccgcc tgtttacatc gggcacccag gactgctcca acaagttcgc cggcagcgaa
     1021 gccgtgttcg gcaccagcac cctgcatccg gtgccggatc tggaacacac ccattacgcg
     1081 ctgatctttg gcgagaatcc caaggtgtcg cacatgagtt ttgtgtcgat ggcgaacccg
     1141 atggaaaaat tccgcgctgc ccgcaagcgc ggcgccgtga tcaaattcat caatccgcga
     1201 cgcatcgaat cggtgacacc ggccaccggc gatctggtgg cgatcaagcc ggatacagat
     1261 ctgtatctgc tggcggcgct gctgcacgaa ctggacgccg ccaatttgtt cgatgaaaac
     1321 atcattgcgc agcacggtga acacattgac ggcttgcgtg cgttcatcaa accctattca
     1381 gcggaaaccg tggcggaggt gaccggcatc gctgccgccg acatcaaaca gatggcgcag
     1441 gattttggcc gcgcgccggc tgcgtgcgcg cacatgtcca ccggcgtcaa catgggccgg
     1501 cagggcacgt tgtgctactg gctgctgcac atgctgtcgt tcgtgaccgg caatctggat
     1561 cgccccggcg gcaatctgta ttcattgggt ttctatcctg cggcgaaagc tggtcgcctg
     1621 aaaacgccga acattttttt caacagcgaa tttggcgaac tgcgtctgat ccggggcgcg
     1681 cttcctggca atctgctgcc cgacctgatc accacgccga ccaatccggt gaaggcgctg
     1741 atcgtgctgg cgggcaatcc gctgctgtcg gtgggtggcg aatcacgcct gcgggaagcg
     1801 ttcggacagc tggagctgat cgtggtgctg gatctgttcc gcaacgccac cggcgagatg
     1861 gcggattacc tgctgccctg caccgacatg ctggaacgca gcgacctcaa catttgcggc
     1921 ctgggcatgc agcagcaacc gttcgtgcaa ttcaccgatg cggtggtgcc ggcccagttc
     1981 gaacggaaag aagagtggtg gattctgaac cggctgcaac aggcgctggg cctgccgtcg
     2041 ctgttcgacc agggcggcgc gggcggcctg tacgatcgcc tgaaccgcat gctgcgttac
     2101 gccgaactgt cgctggatga cgtgcgcaac gcgccgcacc agaccgtggt gctgccaccg
     2161 attcccacgg gccgtttcta ttcggactgg ctgcagaccg aatcgaaaaa agtggattgc
     2221 tgtccgccgc tgtttgacga agcgctgcag cgagcgcaca tcatttttgc ggaattgcag
     2281 cgcgaagatt cgcagcagct gaaactcatc aacctgcgta ccaattacat gcacaacagc
     2341 tggtaccaga acatgacggc acttaaacgg ccaggccagc agttcaatcc gctgcatgtg
     2401 tcgccgcagg atgcgcagcg gctgcagctg caggacgaac aggcggtgac cgtgcgcagt
     2461 ccgtggggcg aaattacggc gcaggtgagg atcgacgatt ccttgcggca agggatagcg
     2521 gccatgagcc acggctgggg caatcagcgc acaccgggca tggcggtggc gcatcagtat
     2581 cccggcacca atgtgaatga gttgctgccc accgggcccg gcagctatga aaaactcagc
     2641 aaccaggcat tcatgacggg gattccggtc gatgtcgaac cggtatcgcc ggcagcagcg
     2701 cttacaccag cttgaactgc agggctttgc gggtggcacc ggtgcggttg tacacgtcca
     2761 gcttgcggta cagattgcgt acgtgagtgc gtaccgtggt gatggacacg tgcagcgtgt
     2821 cggcgatgtc ctggttgcca agcccctgca ccagcagggc cagcacatcc cgttcgcgcc
     2881 ggctgagcgg cacccgcaaa cctgcctgct gcaaacggtc cgggttgaaa tcctccagcg
     2941 gccgcgcctg ctggtataac cggatcaggc ggtcgcgacc aggcagatcg gtttcgcccc
     3001 gcacaaaatc ctgccacagc agaaacgggt ccagcagaat gccagccagc cggtgacttt
     3061 caatctgctg catggcggta cgaaacagcg tttcgcccct gggtttgtcg ccattgcgat
     3121 aaaaatacac ggcccgcagc acatcgattt catacagccg caaccactgc ccgctctgcc
     3181 cgcagaggac cgccagcatt tccagcgagg ccagcacgcc ggtgtactgc cgccggtgca
     3241 ggcgcatctt gatatcccac aggcacccgg tctgctgttg cagactgcca gtgtctgcgg
     3301 cctgcatttc ggtgtgtggc agcagcggcg tcaggtccag atcgtcccct tcatccaccc
     3361 gtcgcagggc gcggtgcagt acttccaccg agggaaacca gtaggagcgg gcgctgcaac
     3421 cctgcttcag caggccaagc ttggaaatgg cctgacgtgc cacatacagt tcgccgcggg
     3481 tcagggccag ttcggcctgc aggttgatca gcagatagtg tttcagaaaa tcctgggtga
     3541 attccggttt ttgcagcagc tgggcaacgc gcggtgccag gtctgccagt tcaccccgca
     3601 cacgggccag caacagctcc agcagatcca gccagtcgta cgcgcccatg aacacgatac
     3661 gtttgtgcat gagccattgc tttacctgtt gcagaaagcg ctcggccaga tccatctcgc
     3721 cagacagcac ctgagcccag caatactgat tggcaaacac caggtagagc gatggcgaat
     3781 cgagcgattc cgccttgtcc agtccggtgg acagcattgg cagggcgcgc aggatctgac
     3841 cggcgtcggc cgcctgatag gcctcccgca gcagcgtcca gggatcagcg ccgcgccaga
     3901 cttcagtcgt caggtccatc aggcggcggc agatggcgtc gccccggttc agcatgcgcg
     3961 aatacagcgc gacttcggcc accagccgct cccactgcgc ctccggcatg gaggcctgca
     4021 gcacagcgct gtcctgcgtt tccagcagaa tcagaatgcg cgagaagaac tgctcgaccg
     4081 ccggcaggtc accgcgatac acggcgcagc gaatggcctt caaaagcagc aatggattgc
     4141 gaattggccg cgccaggtcc agcgtctgaa tccactgtgc cacggcgttc acctgcagct
     4201 gtgcgatcag tacatcgcct tcctgcagca gccagtcttc cacccgctgc cactgggtgg
     4261 cctgtaccag catgtcgatg gcggcctggg agtaccgctg ctgcatcagc cattccgcac
     4321 aggcgatcag tgtggcgctg gtcggcggcg gtgcggggaa gtgggtcagt gtcagccggg
     4381 caaatccggt cccgtgcacc cggatcctgt tgtcggaatc gcgcacgcag tgcacgtagc
     4441 cccattgccg ggccagcgcc aggctgttgt gaagatgtgc caatggtgcg ccggccgcaa
     4501 aaatggcgtc gggcagagcg cccggctttt gcggtaacgc cagcagttcc agcagggatg
     4561 ccggacgggg aaaatcctgc agccgcagcc agcgactgac ctgcatgcaa cccagctcgg
     4621 aacaggctgg tgcagccagc gccggcaggt tccgcagcag ctggaagagg gcagcgggcc
     4681 agcccagggt ggcggtgtac agggcaccgc accagcgcgc cggcaccttg cccgcgcaca
     4741 ccagttgtgc cacggattcc acttcctgcg tgctgaaggt cagcgtgtcg aaatgcagca
     4801 cgtgggcact ccacggtagc gtcgtgctca ccgctgccgg caggcgtgac gtcagcagga
     4861 acaggcgaat cgtgctgtgc cgcgcgccga tggccgatat cagctcgcgc cacggcagac
     4921 agctgccatc ggtcgagcgg tcttccacga caagacagcg cgttgccggt tgttccagtt
     4981 gtgcactgac ggcgacgcgc aattcagcga cgtcctgcac ccggcagacg gaaggtggcg
     5041 tggaaaattc cgcatcggtt ggcggcacgc gggaccagca gatccagcca aaaggctgat
     5101 gttgccgata cagccattgc agcagggtag tgcgtttgcc cgtgcccggc ggcgcatgca
     5161 ggatcagatg cggatgcgcg tcgaagcatt gcaacggcga cgggtgtttg cgcagtagcg
     5221 agaagggtgg aatgcgatta tcagacaaca ggaccggctc ggacatttta ttgttgttgt
     5281 gtcatgccta acgggagtgg cagcccgatg ataacgccaa acgcaggtgc gccagaagtc
     5341 ggctgaacca gaccactgca gtctaggcca ctctccagaa cagcgaaagt agcatgcgat
     5401 tggatcgcac gccttcctct tatcggatga ttttttgccg catgccacag cgttaatagc
     5461 cgaggtctga agcctgcgct ggtgcagaca ggttcgcagt cgaaaaccga aagaagattg
     5521 tccgaacggt cacgcaaaat gtggcgacga ccgcgtgaaa gtgaaaacac ccatcatcgg
     5581 ttcaagccca ttcaggttcg agcccgattg gattccgtcc gtctgaaaca gtgaagtccg
     5641 tgtgcaaatt catcgtccgg gaggaagtcg catccgcaat cgctgcgtag tttgggcctg
     5701 tggcgtcagt ttttcctgac cgctgttcat tagtcgttgc cgatgcagta cccgcaacaa
     5761 caacaaatca aaaagggaag cctcatgaaa aaatccttca agcgattctt cgccctgact
     5821 gcgctgtcat ccgccatcct ggtatccggt tgcgcgcaac cggattccga cgaaccaatc
     5881 gtgcgctggt gggactggga tccattcacc ggcatggcct atctggccga gcaggtactc
     5941 acgctgtaca acaacaattt catcgatacc aacgtggaat acccggccaa tatcgattgt
     6001 ggcgcgacaa cccttgccgt gcgcaccgct gatggcacct gcaatgacat cgaacagaat
     6061 cgcatgggca gcaccggcat ccggtttggt cgcaacgtgc agctggacgc cgcctacccg
     6121 gaccaggcga atctgctgga gccgaacccg cgcaccatca gcc
//
LOCUS       DASUEG010000773         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2152189, whole genome shotgun sequence.
ACCESSION   DASUEG010000773 DASUEG010000000
VERSION     DASUEG010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152189"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..855
                     /locus_tag="VFX11_14630"
     CDS             166..855
                     /locus_tag="VFX11_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX4939911.1"
                     /translation="MTEPLRLSKCVTRQFNCSRREAELLIEGGWVLVDGEPVEEPQFK
                     VLEQTISLHPDAVAEPVEPATILLHKPAGIETGSRELLQLIHPGSRAPDDASRIRPLK
                     KHFSHLQPLLPLEAAAGGLQVLTQDYRVERKLGEEGHRFEQEWIVEVAGCIVTDGLAL
                     LNQSGVKVSWQNETRLRFALKNPQPGQISHLCASAGLTVLAQKRIRIGRIPMAALQPG
                     QWRYLPAHERF"
     gene            954..1121
                     /locus_tag="VFX11_14635"
     CDS             954..1121
                     /locus_tag="VFX11_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939912.1"
                     /translation="MAKVNYNFEKRQKEIARKKKQEQKLAEKRQRKADAEPDQPATEA
                     GADAAPPPADV"
     gene            1114..1719
                     /locus_tag="VFX11_14640"
     CDS             1114..1719
                     /locus_tag="VFX11_14640"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4939913.1"
                     /translation="MSEAAFAALAEDLFRQHYAVLPDFFSPAQILAWHDDATAASRQG
                     LFRAAAVGRNIALLRDTSIRGDTIHWLEPDTRIPAQQTYLAFLETLRLRLNEQFMLGL
                     FAAECHYAHYVAGSFYKKHLDRHQGSRDRVVSVICYLNGDWQARDGGELRLYLGQDTR
                     DVPPLAGTLVCFFSERIPHEVLVVHRERLSLTAWLRVWPAD"
     gene            1778..>2317
                     /locus_tag="VFX11_14645"
     CDS             1778..>2317
                     /locus_tag="VFX11_14645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4939914.1"
                     /translation="MTDRSYIAPLDGIRGLAILLVLGHQGAFASAIDGNQVLGGLLMR
                     VLDAGWVGVQLFFVLSGFLITGILADAKGADIGRSFKHFYLRRAVRIFPVYYVTLVVL
                     FIAAALMTDGPDWLENTYDHKIWYLLYLNNWITPFVEELLGHFWSLAVEEQFYMLWPL
                     VVFTVNRQSLVWICLGLVVL"
BASE COUNT          415 a          678 c          750 g          474 t
ORIGIN      
        1 actgacgtgc gaaaccaggc ctgacagcat gctgagctcc ggaaggtttt tgttttgggc
       61 ggatcttgaa ctgttatttc agtgtagaag gcgtatccca acctgcaatt gtgtcgattc
      121 cggcccgggc atgcacagcg ggtgcctgag cggttacaat gccccatgac cgaacccctg
      181 cgcctgtcca agtgtgtcac ccgccagttc aactgctccc gccgcgaggc cgagctgctg
      241 atcgagggcg gctgggtgct ggtggacggc gaaccggtgg aagaacccca gttcaaggtg
      301 ctggagcaga ccatcagcct gcatccggac gcggtagccg aaccggtgga gcctgcgacg
      361 attctgctgc acaagcccgc cggcattgaa accggcagcc gcgagctgtt gcagctgatc
      421 catccgggtt cccgcgcgcc agacgatgca tcccgcatcc gcccgctgaa aaagcatttt
      481 tcccacctgc aaccgctgct gccgctggaa gctgctgccg gtggcctgca ggtgctgacc
      541 caggattacc gggtggagcg caagctgggg gaagagggcc atcgtttcga acaggaatgg
      601 attgttgagg tggcaggctg tatcgtgacg gacggccttg ccctgctcaa ccagtcgggg
      661 gtcaaggtca gctggcagaa tgaaacccgc ctgcggttcg cactgaagaa cccgcagccg
      721 gggcagatca gccatttgtg cgccagcgct ggcctgaccg tgctggcgca gaaacgcatc
      781 cggatcggcc gtattcccat ggccgccctg caaccggggc agtggcgcta cctgcctgcc
      841 cacgaacgct tttgaacgcc cggcagcagc gggcacccgc accagacttg gccttcgcct
      901 gatcctgtca ggccctgcgc ttgtttccca cacttcaccc aaaaggtttg cgcttggcca
      961 aggtcaatta caacttcgaa aaacggcaga aagaaatcgc ccggaaaaag aagcaggaac
     1021 agaagctggc cgagaaacgc cagcgcaagg cggatgcgga acccgatcag ccagccactg
     1081 aggctggtgc cgacgccgca ccgccgcctg cggatgtctg aggcggcttt tgccgcattg
     1141 gcagaggatc tgttccggca gcattatgcg gtgttgccgg attttttcag cccggcgcag
     1201 attctcgcat ggcacgatga tgccacggcg gcctcccggc aggggctgtt ccgggcggcg
     1261 gcggtcgggc gcaacatcgc cctgctccgc gacacctcca ttcgtggcga cacgattcac
     1321 tggctggaac ccgacacgcg gattccggcg caacagacgt atctggcatt tctggaaacc
     1381 ttgcggctgc ggctgaatga gcagttcatg ctgggcctgt ttgcggcgga gtgccattac
     1441 gctcactacg tggccggcag cttctacaag aagcacctgg atcgccatca gggcagtcgc
     1501 gacagggtgg tcagcgtgat ttgctacctt aacggcgact ggcaggcgcg ggacggcggc
     1561 gagctgcgcc tgtatctggg ccaggacacc cgggatgtgc cgccgctggc ggggacgctg
     1621 gtatgctttt tctcggagcg gattccccat gaggtgctgg tcgtccatcg cgagcgcctc
     1681 agcctgaccg cctggctacg ggtgtggccg gcggattgac cggggcttgc agtctggtat
     1741 cgtggcgccc gcagtagctg accaagaggt ggttaacgtg acagaccgat cctatatagc
     1801 gccgctggat ggcatccggg ggctggcgat cctgctggtg ctgggtcacc agggggcatt
     1861 tgccagcgct atcgatggaa accaggtgct gggcggactt ctgatgcgtg tgctggatgc
     1921 gggctgggtg ggggtgcagc tgttctttgt gctgtccggc tttctgatca ccggtattct
     1981 ggcggatgcc aaaggcgccg atatcggccg ctccttcaag catttctacc tgcgccgtgc
     2041 cgtgcgcatt tttccggttt actacgtgac actggtggtg ctttttattg ccgccgcttt
     2101 gatgacggac ggaccggact ggctggaaaa tacctacgac cacaaaatct ggtacctgct
     2161 gtatctgaac aactggataa cgccgtttgt cgaagagttg ctgggccatt tctggtcgct
     2221 ggcggtggaa gaacagttct atatgctgtg gccgctggtg gtgtttaccg tcaaccgcca
     2281 atcgctggtg tggatttgtc tggggcttgt cgtgctg
//
LOCUS       DASUEG010000774         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1886907, whole genome shotgun sequence.
ACCESSION   DASUEG010000774 DASUEG010000000
VERSION     DASUEG010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886907"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1076)
                     /locus_tag="VFX11_14650"
     CDS             complement(72..1076)
                     /locus_tag="VFX11_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4939915.1"
                     /translation="MPFTITFHPANLQLQVPDGETVLQAALNQGFYFPWGCVAGSCTL
                     CEGKLLRGRVRLKPGNDIITPESQRADALLCCLAYPLENCDIEVTRVLAPGQIPLQTL
                     TAKIIRIEQVNADVKIVHFLLPAGKRPSFSAGQYLELLIDADTSASFSIGNAPRPDRT
                     LELHIRANPDSSSYPRLAPRLRENELIKMRLPLGDVTLHSLKGADRILLLAGSTGFSQ
                     IKALLEALLQGGDTRPIHLYWGGRVFADLYLHHWATQMAAQYANLHYVPVISDQADWQ
                     GRKGFVHKAVLDDIQDFAGWKVVGGGSPGMVYAALDDFVAAGMQAEQLVSDVFAYAPR
                     "
     gene            complement(1151..>2098)
                     /gene="rho"
                     /locus_tag="VFX11_14655"
     CDS             complement(1151..>2098)
                     /gene="rho"
                     /locus_tag="VFX11_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4939916.1"
                     /translation="KDGERYFALLKVNTVNFEDPEKQRHKVHFDNLTPLYPDERLKME
                     IDDPTLKDRSGRVIDIVAPLGKGQRALIVAPPRTGKTVLLQNIARSISANHPECFLIV
                     LLIDERPEEVTDMQRTVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKRDVVILL
                     DSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALID
                     TGSRMDEVIFEEFKGTGNQEIHLDRRIAEKRVFPAININRSGTRREDLLTSEDELKKM
                     WILRKILHPMDEIAAMEFLIDKMRDTKTNEEFFESMKRK"
BASE COUNT          383 a          594 c          666 g          455 t
ORIGIN      
        1 attggctatc tggcgggatt gggttaccgc gacctttgga ctgaagaatc caaccgtatc
       61 aattcctgaa ttcaccgcgg cgcatacgca aaaacatccg acaccagctg ctccgcctgc
      121 attcccgccg caacaaaatc gtccagtgcg gcatacacca tgcccggtga gccgccgccc
      181 accactttcc agccggcaaa atcctgaatg tcgtccagca cggctttgtg cacaaagccc
      241 ttgcggccct gccagtccgc ctgatccgaa atcaccggca cgtaatgcag attggcgtat
      301 tgcgccgcca tctgcgtcgc ccaatgatgc aggtacagat ctgcaaacac gcgcccgccc
      361 cagtacaggt gaatcggccg cgtatcgccg ccttgcagca gggcttccag cagggctttg
      421 atctgggaaa agccggtgct gccggccagc agcagaatcc ggtccgcgcc cttgagggag
      481 tgcagggtga catcgcccaa aggcaggcgc attttgatca gttcattttc ccgcaggcgc
      541 ggcgccagcc tggggtagct gctgctgtcc ggattggcgc ggatatgcag ttccagcgtg
      601 cggtcggggc gcggtgcgtt accgatggag aacgaggcgc tggtgtcggc gtcgatcagc
      661 agttccagat actggccggc gctgaatgac gggcgtttgc cggcgggcag caggaaatgc
      721 acgatcttga cgtcggcgtt gacctgttcg atgcggatga ttttggcagt cagtgtctgc
      781 agggggatct gtcccggtgc cagcacgcgt gtgacctcga tgtcgcaatt ttccaaagga
      841 tacgccagac agcataacag cgcatctgca cgttgggatt cgggggtgat gatgtcgttg
      901 ccaggtttca gccgcacgcg gccgcgcagc agtttgcctt cgcacagggt gcagctgccg
      961 gcgacacagc cccagggaaa atagaagccc tggttgagtg cggcttgcag aacggtttcg
     1021 ccatcgggta cctgcagttg caggttggcg gggtggaagg tgatggtgaa gggcatgtca
     1081 gtagaagaca tcgtggcggg tgcgaaggga tgttcacacc cgccacgatg tcagccgatt
     1141 ggttgccgtt ttacttgcgc ttcatcgact caaagaattc ttcgttggtc ttggtgtcgc
     1201 gcatcttgtc gatcaggaat tccatcgcgg cgatctcgtc catcgggtgc aggatcttgc
     1261 gcagaatcca cattttcttc agttcgtctt cgctggtcag caggtcttcg cgacgggtgc
     1321 cggagcggtt gatgttgatc gccgggaaca cgcgtttttc cgcgatgcga cggtccaggt
     1381 gaatttcctg gttgccggtg cccttgaatt cttcgaagat gacttcgtcc atgcggctac
     1441 cggtatcgat gagcgcggtc gcaatgatcg tgagcgatcc accttcctcg atgttacggg
     1501 ctgcaccgaa gaagcgcttc gggcgctgga gcgcgttggc atcgacaccg ccggtcagca
     1561 ccttgccgga gctgggcaca accgtgttgt aggcgcggcc gaggcgcgtg atcgagtcga
     1621 gcaggatgac cacgtcgcgc ttgtgctcga cgaggcgctt tgccttctca atcaccattt
     1681 ccgcgacctg gacgtggcgg gatgccggct cgtcgaatgt cgaggagatg acttcgccct
     1741 tcacggtgcg ctgcatgtcg gtcacttctt caggtcgttc gtcgatcagg agaacgatca
     1801 ggaagcattc agggtgattg gccgaaatgg agcgggcgat gttctggaga agaacggtct
     1861 tgccggtgcg tggcggcgcc acgatcagtg cgcgctggcc cttgccgagc ggcgcgacga
     1921 tgtcgatcac ccggcccgag cggtccttca gggtgggatc gtcgatctcc atcttcaggc
     1981 gctcgtccgg atagagcggc gtcaggttgt cgaaatgaac cttgtggcgc tgcttttccg
     2041 ggtcttcaaa attgaccgtg ttcaccttga gaagggcgaa gtaacgctcg ccatcctt
//
LOCUS       DASUEG010000775         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_760540, whole genome shotgun sequence.
ACCESSION   DASUEG010000775 DASUEG010000000
VERSION     DASUEG010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760540"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFX11_14660"
     CDS             <1..720
                     /locus_tag="VFX11_14660"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4939917.1"
                     /translation="SLILTGTDLVSSQQALALCAEHGDGLYCTAGVHPHYAKEWSDQH
                     REEMLALTRHDRVVAIGETGLDYNRNLSPPEQQRRVFEQQLALAADTGLPLFLHERDA
                     AEDQVALLRRFRNRISGGVAHCFTGTAEVMQQYLALDLHIGITGWICDERRGLHLREQ
                     VKEIPLNRLLLETDGPYLLPRDLQPKPKSRRNEPKYLPHIATVVAECVGMSPEELAAV
                     TTANAEGLFGLKSNTRSAGKT"
     gene            complement(765..>2096)
                     /locus_tag="VFX11_14665"
     CDS             complement(765..>2096)
                     /locus_tag="VFX11_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4939918.1"
                     /translation="PVMANIQDITDKIKEAYKDESDFSELEAVVGQEDPLETVELIIE
                     DDDDVSVEELLKSTEEPPAVRLVNAIIIEAVRLGVSDIHVHPRSKHVAVRYRIDGILQ
                     DKIHVPLNYLRSIVSRIKVMAELDISERRRPQDGRITVKTPLRIVDLRISTLPTINGE
                     KVVLRLLDKHGSIHGLEEQGFSPDELLKVKTMIQQPQGIILVTGPTGSGKTTTLYTLL
                     QHNATPEKNYVTIEDPVEFYMDLAGQVSVHGKINLDFSAILRAILRQDPDVILLGEIR
                     DLETAEVAFHAAMTGHLVYSTLHTNSALATVSRLVDIGLKPFVVASGLTGIIAQRLLR
                     KVCRFCRQEVTPDPEMLKLLGRGFDTLTTVCKGAGCERCHQSGYGGRIPIYEVLLIND
                     ELREAIHELGASRNRLVAAENQSSLFDSALQRVRDHLTTPEEVLRVLGPRF"
BASE COUNT          443 a          629 c          593 g          431 t
ORIGIN      
        1 agcctgattc tcaccggcac cgatctggtg tcgtcgcaac aggcgctcgc actgtgcgcg
       61 gagcatggcg acggcctgta ttgcactgcc ggtgtgcatc cccattatgc gaaggaatgg
      121 tcggaccagc atcgcgaaga aatgctggcg ctgacccgcc atgatcgcgt ggtggcgatc
      181 ggcgaaaccg gactggatta caaccggaat ctgtcgccgc cggagcagca gcgccgggtc
      241 ttcgaacagc aactggcgct ggcggcagac accggactgc cgctgttcct gcacgagcgg
      301 gatgcggcgg aggaccaggt tgcgctgctg cgacgcttcc gcaaccgcat cagcggcggc
      361 gtggcgcact gttttaccgg cacggctgag gtgatgcagc aatatctggc gctggatctg
      421 catatcggta tcactggctg gatctgcgac gaacggcgcg ggttgcacct tcgggaacag
      481 gtgaaagaaa ttccgctaaa ccggctgctg ctggaaacgg acggccccta cctgctgccc
      541 cgtgatctgc agcccaagcc gaaatcccgt cgcaatgagc cgaaatatct gccgcatatt
      601 gccacggtag tggcggagtg tgtggggatg tcgccggaag aactggcagc agtgactacg
      661 gccaatgcag aggggctctt tggactgaaa tccaataccc gttcagctgg aaagacctga
      721 aaaccggatg gagcgcgatt ctgccgcagg acaggcaaca accgtcaaaa gcgcggtccc
      781 agcaccctca atacctcctc tggcgttgtc agatggtcgc gcaccctctg caacgcactg
      841 tcaaacagac tggactgatt ctccgccgca accagccggt tgcgcgacgc gcccagctcg
      901 tggatggcct cgcgcagctc atcgttgatc agcaacactt catagatcgg aatcctgccg
      961 ccgtagccgg actggtgaca gcgctcgcaa ccggccccct tgcaaacagt ggtcagcgta
     1021 tcaaaaccac gccccagcaa cttcagcatt tcagggtccg gtgtcacttc ctgacggcag
     1081 aatctgcaga ccttgcgcaa aaggcgctgc gcaatgatgc ccgtcagacc ggacgccacg
     1141 acaaagggtt tcaggccgat atccaccagc cgggatacgg tggccagcgc ggaattggta
     1201 tgcagtgtcg agtacaccag atgtcccgtc atggccgcgt gaaacgcaac ctcggctgtt
     1261 tccagatcac gaatctctcc cagcagaatg acgtcagggt cctggcgcag tatcgcgcgc
     1321 agaatggcgg agaaatccag attgattttg ccatgcacac tcacctgccc tgccagatcc
     1381 atgtagaact ccaccgggtc ctcaatggtg acgtagttct tttccggcgt ggcattgtgc
     1441 tgcagaagcg tataaagggt ggtggtttta ccgctcccgg tgggtcctgt caccagaata
     1501 atgccctgtg gctgctggat catcgttttg accttcagca actcgtccgg cgaaaacccc
     1561 tgttcttcca ggccgtgaat cgaaccgtgc ttgtccagca ggcgcagtac cactttctcg
     1621 ccattgatgg tgggcaatgt cgaaatccgc aaatcgacta tacgcagcgg cgtctttacc
     1681 gtgatgcgcc cgtcctgtgg ccgccgccgc tctgaaatat ccagttcggc catgactttg
     1741 atacgggaca cgatggaacg gagataattg agcggtacgt gaatcttgtc ctgcagaatt
     1801 ccatctatcc ggtaccggac ggcaacatgc tttgaccgcg ggtgaacgtg aatgtcgctg
     1861 acgcccagcc gtaccgcttc gatgatgatg gcattgacca gccgaaccgc aggcggctcc
     1921 tcggtacttt tcagcagttc ctctacactg acgtcgtcat cgtcctcgat aatcagctca
     1981 actgtctcaa gcggatcttc ctgaccgaca accgcttcca gttcggaaaa gtcagattca
     2041 tccttgtatg cttccttgat cttgtccgta atgtcctgaa tgttcgccat cacagg
//
LOCUS       DASUEG010000776         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_606130, whole genome shotgun sequence.
ACCESSION   DASUEG010000776 DASUEG010000000
VERSION     DASUEG010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606130"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFX11_14670"
     CDS             complement(<1..227)
                     /locus_tag="VFX11_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the hydrolytic cleavage of a
                     carbon-halogen bond in N-ethylammeline; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX4939919.1"
                     /translation="MPESRPAGTVVSTLIKAPWIVTMDGARRVLENHCILIDKGRIVA
                     LLPQDQPQYSAQEIIDLPDQVLMPGLINAHC"
     gene            324..1394
                     /gene="mtnA"
                     /locus_tag="VFX11_14675"
     CDS             324..1394
                     /gene="mtnA"
                     /locus_tag="VFX11_14675"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX4939920.1"
                     /translation="MSPFPTVSAIEWRDDTLWLLDQRLLPLQEVWLDHADVHAVVNSI
                     RDMVVRGAPAIGITAAYGVVIAARARRAENPQHWQQLLVADFEALAASRPTAVNLFWA
                     LDQMKTALEKLDRNQDPVAQLLAEAMRIHESDLAANHTMGHLGANLIRHYGMPAKGVI
                     THCNTGSLATGGYGTALGVIRSAWKAGLIEQVYADETRPWWQGARLTAWELLKDGIPV
                     TLHADSVAATLMQQGKVSWVIVGADRITANGDVANKIGTYSLAVLARHHGVRFMVVAP
                     TTTFDPSLAHGSAIPIEERGPEEVVSVKGVRVAPEGVAVYNPVFDVTPAELIDAIVTE
                     KGVVEHPSRDGIAALLAQASIH"
     gene            1666..>2168
                     /locus_tag="VFX11_14680"
     CDS             1666..>2168
                     /locus_tag="VFX11_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4939921.1"
                     /translation="MSELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELRNDWNKAYKKSARVVGDVIGKYHPHGDTAVYDTIVRLAQDFAMRYTL
                     VDGQGNFGSIDGDNAAAMRYTEVRMQKLTNEMLADIEKETVDFQPNYDESLSEPKVLP
                     ARIPNLLV"
BASE COUNT          420 a          660 c          679 g          409 t
ORIGIN      
        1 tggcaatggg cgttgatcag gcctggcatc agcacctgat cgggcagatc gatgatttcc
       61 tgcgcgctgt actggggctg gtcctgcggc agcagcgcga cgatgcggcc cttgtcgatc
      121 aggatgcagt gattttccag cacgcggcgc gcgccatcca tggtgacaat ccacggagcc
      181 ttgatcaggg tgctgacgac ggtgccggcg gggcgggact cgggcatggg cagggatttc
      241 catccgcaat ttgaaatagc gcaatcttac cccagccacc gtataattca cgcttttccg
      301 caaccggccc aggagttttt tctatgtccc cgtttcccac cgtgagtgcc atcgaatggc
      361 gtgacgacac cctctggctg ctggatcagc gtctgctgcc cctgcaggag gtgtggctgg
      421 atcacgcgga tgtgcacgcc gtggtgaact cgatccggga catggtggtg cgcggcgcgc
      481 cggccattgg catcaccgcg gcctacggtg tggtgatcgc ggcccgtgcc cggcgggcgg
      541 aaaatccgca gcactggcag caactgctgg tggcggattt cgaggcgctg gcggcgtccc
      601 gtcccaccgc cgtcaatctg ttctgggcgc tggaccagat gaagaccgcg ctggaaaagc
      661 tcgaccgcaa ccaggacccg gtggcgcagc tgctggcaga agccatgcgc attcacgaat
      721 ccgatctggc cgccaatcac accatgggcc atctgggcgc caacctgatc cgccattacg
      781 gcatgccggc caagggtgtc atcactcact gcaatacggg ttcgctggcc acgggcggct
      841 atggcacggc gctgggcgtc atccgttccg catggaaggc gggcctgatc gagcaggtct
      901 acgccgatga aacccggccc tggtggcagg gcgcgcggct gactgcgtgg gagctgctga
      961 aggacggcat tcccgtcacg ctgcacgccg acagcgtggc ggccaccctg atgcagcagg
     1021 gcaaggtgag ctgggtgatc gtgggcgctg accgcatcac cgccaacggc gacgtggcca
     1081 acaagatcgg tacttacagc ctggcggtgc tggcccgtca ccacggtgtg cgcttcatgg
     1141 tagtggcgcc cacaaccacg tttgatccca gtctggccca cggcagcgcc attcccatcg
     1201 aggagcgcgg tccggaagaa gtggtgtccg tgaaaggtgt gcgggtggcc ccggaagggg
     1261 tggcggtgta caacccggtt tttgacgtga ccccggctga gctgatcgac gccatcgtca
     1321 ccgaaaaagg ggtggtggag cacccgagcc gggacggaat cgctgccctg ctggcccagg
     1381 cttccattca ttgatcaggc ccccgaaacg gacccggagc gaacgcttca gatacttgat
     1441 tttttgcgca aaaaacccgc cgttccctta gcctatcagc gcccgattat ggtatgatct
     1501 ggcgctttca tccggcccgt tttcagatgt tgcgggccgg ttctggtaac aacccggaca
     1561 ccacaataaa aacggcgctg aacggcattg tccggttcac cggtccccag cgggatcact
     1621 gcgcggaacc tccgtcgcag gcgtcatttt caggtcagga acccgatgtc agaactagcc
     1681 aaagaaatcc taccggtcaa catcgaagac gaactcaagc aatcctatct cgactacgcc
     1741 atgagcgtga tcgtgggccg tgcgttgccg gatgtgcgcg acggcctcaa gcctgtgcac
     1801 cggcgcgtgc tgttcgccat gcacgagctg cgcaacgact ggaacaaggc ttacaagaaa
     1861 tcggcccgtg tggtcggtga cgtgatcggt aaatatcacc cgcacggcga tacggccgtt
     1921 tacgatacga tcgtccggct cgcccaggat tttgccatgc gctacactct ggtcgacggt
     1981 cagggcaatt tcggctcgat cgacggtgat aacgccgcgg ccatgcgcta caccgaggtt
     2041 cggatgcaaa agctgaccaa tgaaatgctg gccgatattg aaaaagaaac ggtcgatttt
     2101 cagccgaact acgacgaatc gctctcggaa ccgaaggttc tgccggccag gatcccgaat
     2161 ctccttgt
//
LOCUS       DASUEG010000777         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_937302, whole genome shotgun sequence.
ACCESSION   DASUEG010000777 DASUEG010000000
VERSION     DASUEG010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937302"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFX11_14685"
     CDS             <1..345
                     /locus_tag="VFX11_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4939922.1"
                     /translation="PELEHEQVTTTDLQLFHNGPEVQVALTLFQNVQKDLIVRAVQGS
                     TITFVNQGELRIRGLELEGKTVLGERWYLLGSATWQKNEDGNGVENVSLQPQHMLKAG
                     IAYRALDWSVGL"
     gene            382..615
                     /locus_tag="VFX11_14690"
     CDS             382..615
                     /locus_tag="VFX11_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939923.1"
                     /translation="MVPTRLLVNPEADAYDLLSLNFNYRMRELGDAQLEIYLDNLLDE
                     TIYRPTLPGVGLVSNTTSYRDGRYGQIGITLPF"
     gene            complement(631..>2204)
                     /locus_tag="VFX11_14695"
     CDS             complement(631..>2204)
                     /locus_tag="VFX11_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4939924.1"
                     /translation="LLSQRLLDGVKVNGSTYQKIVLVKDLVADILPPPEYIIESHLSV
                     LQLLATRNKAKQEAWLENLERVEKEFRARHDFWLEDTILPPELKEQITESVFKPAEEY
                     YQVLHAEFLPPLQKGQLSDAKIAFNRLDELYSAHRKAVDKLVADSLAEQAAIEQQTRS
                     AVDQFESILLTILVVSTLLVSAVVVVILQGLRRRFGGEPVQVEQVLQKVAQGDFSGRG
                     VLLEQTPEDSVLGQALNMRNQLRQLLGSFADMAVALDQSAGGLQKDAKRGEQDAQVQQ
                     DDARTMAAAIEELSVSIDQLSRNSSQQLSLARGAARESDAGRQMVQQCGQVLQRLCDF
                     VQKLSDNVQRLSDNSKRIESITLTIKDVAEQTNLLALNAAIEAARAGESGRGFAVVAD
                     EVRSLSARTRQSTHEIDSIVARIREEVNLIVGSVEDGVRLSNQGLQSMEQASQSIDNL
                     QQGAQELEQYSQTVATALSQQNQATQTLAQDVSHIAQRAQQQAAQARNASASAQELAG
                     MANTITGQLSRFSLR"
BASE COUNT          375 a          713 c          675 g          441 t
ORIGIN      
        1 ccggaactgg agcacgaaca ggtcaccacc acggatctgc agctgtttca caacggcccc
       61 gaggtgcagg tggccctaac cctgtttcag aacgtgcaga aggatctgat cgtgcgtgcc
      121 gtacagggca gcaccatcac cttcgtcaat cagggtgagc tgcgcattcg tggcctggaa
      181 ctggaaggca aaaccgtgct gggggagcgc tggtatctgc tgggctccgc cacctggcag
      241 aaaaacgagg atggcaacgg ggtggagaat gtgtcgctgc agccgcagca catgctgaag
      301 gcgggaattg cctaccgcgc gctagactgg tcggtggggc tgtaagacgt ccactccggc
      361 cagtacccgg acaatcaggt agtggtgccc acccggctgc tggtcaatcc ggaagcggac
      421 gcgtacgacc tgctgtcact caatttcaat taccggatgc gggaattggg cgatgcccag
      481 ctggaaatct acctggacaa cctgctggac gaaaccattt accgccccac cctgccgggt
      541 gtcggcctgg tgtccaatac cacgtcctac cgtgacggcc gctacggcca gatcggcatc
      601 acgctgccgt tctgaaccgg ttcgttcatc tcagcgcagg gaaaaccggc tcagctgtcc
      661 ggtaatcgta ttggccatgc ccgccagttc ctgcgccgac gcgctggcgt tgcgggcctg
      721 agccgcctgc tgctgggctc gctgggcaat gtggcttacg tcctgggcca gggtctgggt
      781 ggcctggttc tgctgcgaca gcgcggtggc cacggtctgg gaatactgct ccagttcctg
      841 ggccccctgc tgcaggttgt cgatggactg gctggcctgc tccatgcttt gcaggccctg
      901 gttcgacagg cgcacgccat cctccaccga tcctacgatc aggttcacct cttcgcggat
      961 gcgcgcgacg atgctgtcga tttcatgggt ggactgccgc gtacgggccg acaggctgcg
     1021 cacctcgtcc gccaccaccg cgaagccgcg accggattcc cccgcccgcg cggcctcgat
     1081 cgctgcgttc agtgccagca ggttggtctg ctccgccaca tccttgatgg tgagagtgat
     1141 ggattcgatg cgcttggaat tgtcactcag gcgctgcacg ttgtccgaca gcttctgcac
     1201 gaagtcgcac agccgctgca gcacctgacc acactgctgc accatctggc ggccggcgtc
     1261 ggactcgcgg gccgcgccgc gggccaggct cagttgctgg gaagaattgc gtgacagctg
     1321 gtcgatgctc accgacagtt cctcgatggc agcggccatg gtgcgggcgt catcctgctg
     1381 cacctgtgca tcctgctcgc cgcgtttggc atccttttgc aggccgccgg cggactggtc
     1441 cagtgccacc gccatgtcgg cgaacgaacc cagcagctgg cgcagctgat tgcgcatgtt
     1501 cagcgcctgt cccagcacgc tgtcttccgg tgtctgttcc agcagtacgc cgcgcccgga
     1561 aaaatccccc tgtgcgactt tctgcagtac ctgctccacc tgcactggct cgccgccgaa
     1621 acgccggcgc aggccctgca ggatcaccac caccaccgcc gataccagca gcgtcgacac
     1681 caccagaatc gtcagcagta tggattcgaa ctgatccacc gcgcttcggg tctgctgctc
     1741 gatggcggcc tgttccgcca gggaatccgc caccagcttg tccacggcct tgcggtgggc
     1801 gctgtaaagt tcatccaggc ggttgaaggc gatcttggcg tccgacaact ggcctttctg
     1861 cagcggcggc aggaactcgg catgcagcac ctggtagtac tcctccgccg gcttgaacac
     1921 ggattcggtg atctgctctt tcagctccgg tggcaggatg gtgtcctcca gccagaaatc
     1981 gtgacgggcc cggaattcct tctccacccg ttccaggttc tccagccagg cctcctgctt
     2041 ggccttgttg cgggtggcca gcagctgcag cacgctcagg tgactttcga tgatgtattc
     2101 cgggggtggc aggatgtcgg ccaccagatc ctttaccagg acgattttct gataagtgga
     2161 accatttact ttaacgccgt ccagcaggcg ctgggacagc aggg
//
LOCUS       DASUEG010000778         6108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_142090, whole genome shotgun sequence.
ACCESSION   DASUEG010000778 DASUEG010000000
VERSION     DASUEG010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142090"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1274
                     /locus_tag="VFX11_14700"
     CDS             129..1274
                     /locus_tag="VFX11_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4939925.1"
                     /translation="MSRLHYLDHLRAHMMLLGILFHCALSYSHFEPGEDWAYVEPASH
                     HLFDLACGTIHLFRMPAFFIVAGFFAALILERKSARHFMRDRSLRVVLPLLLLALPNE
                     WITRAIYASSRTLSPAAVPVATEMFEWHHLWFLYFLALFYGIHLLLQSLSGRLGWQLP
                     ARWQQTPVTLLALPFGLIMGLTVYGNGTLADAAPIALLPDPGQFLFYLACYSLGALLY
                     GHPGLTGRQPALLGIGSATRHWLFWSLLSLASANIVLFASQIGSNGFNPVSALSLGMA
                     KVYAALLVMALYQRYLRQENALVRYLSNSSYWLYLIHFPLAIALPPLLHDWPLAPTLK
                     FGLVLVATLGLGLLSYEFLVRDTPVGALLNGRRIPRRRSTSLAPAPW"
     regulatory      1496..1603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            1667..2755
                     /locus_tag="VFX11_14705"
     CDS             1667..2755
                     /locus_tag="VFX11_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4939926.1"
                     /translation="MRNLLKALILAPFIRTVLTVFCAALAVVGIAHAEERKSFSVCWS
                     IYVGWMPWDYGAQQGIVKKWADKYGIAIDVVQINDYVESINQYTAGKYDGCTMTNMDA
                     LTIPAAGGVDSTALIVGDFSNGNDGVVLKGKKSLADIKGQSVNLVELSVSHYLLARGL
                     ETVGLSEKDLKVVNTSDADLVAAFATPDVTSVVTWNPLLSEILTMPNSNKVFDSSQIP
                     GEIIDLMVVNSDVLKANPKFGKALVGAWYEIMATMSAQDAKGKAAREFMAKASGTDLA
                     GFEAQLASTKMFYTAKDAVAFTTSAQLKTTMEYVAKFSFDHGLLGDGAADAGFIGVET
                     AAGLYGSSKNVKLRFDPSYMQMALDGKL"
     gene            2793..3626
                     /locus_tag="VFX11_14710"
     CDS             2793..3626
                     /locus_tag="VFX11_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016960970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4939927.1"
                     /translation="MKKIINLQPHNGTRWLLALLPFLLLLLLYVAASNARLADNPGDK
                     LLPAMGSFAEAIQRMAFEPDKRSGDYLLWLDTGSSLIRLGLGVGISALAGLVFGILNG
                     AIPFARAPLSPLVTFLSLIPPMAILPILFIIFGMDELAKVMLIIIGITPALIRDMQQR
                     VLEIPAEQLIKAQTLGASSWQIILRVVLPQILPRLLASVRLALGSAWLFLIAAEAIAS
                     TDGLGYRIFLVRRYLAMDVILPYVIWITLLAFFIDYGLKKISQWSFPWFHSQTGDGGK
                     S"
     gene            3623..4420
                     /locus_tag="VFX11_14715"
     CDS             3623..4420
                     /locus_tag="VFX11_14715"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4939928.1"
                     /translation="MSYIQIRNLWKEYADQVVLERINARVNKGDFITIVGASGCGKTT
                     FLKMLLGTESPSRGAILLEDAPIPDEPSQQRGIVFQRYSVFPHLTVLKNVMLARELEH
                     SPLLGKLFGSARRTAKQEALAMLEKVGLTAAINKYPHELSGGMQQRLAIAQSLIRKPA
                     ILLLDEPFGALDPGIRADMHKLVLELWKQHQLTIFMITHDLKEGFLLGTRLWVFDKPR
                     HDPQAPGAFGARITYDIPVGQSDRSLYQKIDQQLQVTQELAMEGNAA"
     gene            4417..5151
                     /locus_tag="VFX11_14720"
     CDS             4417..5151
                     /locus_tag="VFX11_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEX4939929.1"
                     /translation="MTSALFQESLYQYRMPGAAHWSLKMRRGTILQLTDVEGGANVGL
                     LFYNPEDLLERYNAPDTLKCQHTFKLTAGNCLYSDMGRIFASIVADDFGWHETVCGNS
                     DAKKTAARWGRHSYQDFRNEWYLNGHDSFLVELAKYGLGEKDMAANLNLFSKVVVDDA
                     GNLSLDEGAARAGASISLRFEMDTLVLLHTCPHPLNRASEFPRKPVQCEIFRAAPVAE
                     DDFCRNFRPENQRGFENNRLYHLGRV"
     gene            5162..5800
                     /locus_tag="VFX11_14725"
     CDS             5162..5800
                     /locus_tag="VFX11_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEX4939930.1"
                     /translation="MIKESTLSPADAMHKEIVPAGDYWMHKLKHGQTLRILDLEGNQA
                     ADTLFYSQKDPAERYSAMDTIREQGNVYLTAGTKLLSNRGNLMLEITADTCGRHDTLG
                     GACATESNTVRYALEKRCMHACRDSYLLAVAQNEKFGMSKRDITHNINFFMNVPVTAD
                     GGLTFADGISAPGKYVEMKAAMDVIVLISNCPQLNNPCNGYNPTPVEVIIWN"
BASE COUNT         1253 a         1725 c         1828 g         1302 t
ORIGIN      
        1 gtagacaggc ggcacgcaaa taaccggcgc ctgcacgttc gtgcaatcgg aaattggagc
       61 ggatcatcac attgtggtca ctttgaatct gctatcgtgc cctgccatcg caacggcatg
      121 gaaagcctat gagcagactt cactatctgg atcacctgcg cgcgcacatg atgctgctgg
      181 gcatcctgtt tcattgcgcg ctcagctaca gtcacttcga accaggggaa gactgggcct
      241 atgtcgagcc cgcgtcccat catctgttcg acctggcctg cgggaccatt cacctctttc
      301 gcatgccggc gttctttatc gtggccggct ttttcgctgc cttgatcctg gagcgcaagt
      361 ctgcccggca cttcatgcgc gatcgcagcc tgcgggtcgt gttgccgctg ctactgctgg
      421 cactgcccaa cgagtggatc acccgcgcga tttacgccag ctctcgcaca ctttcccctg
      481 cggctgtgcc ggtagccact gaaatgttcg aatggcacca tctatggttt ctgtacttcc
      541 tggccctgtt ttacggtatt catctgctgc tgcaatcatt gtccggacgc ttgggctggc
      601 aactgccggc acgctggcag cagacgccgg tgaccttact ggccctgccg ttcggactga
      661 tcatgggatt gacggtctac ggaaacggga cgctggcgga cgcagcgccc attgcgctgc
      721 tgccggaccc ggggcaattt ctgttttatc ttgcctgcta cagcctgggc gccctgttgt
      781 atggacaccc gggactgacc ggccggcaac ccgcgctgct cggcatcggc tccgccaccc
      841 ggcactggtt gttctggagt ctgctgtccc tggccagtgc caacatcgta ctgtttgcca
      901 gccagatcgg gagcaacggc ttcaatccgg tctcggcact gagtctgggc atggccaaag
      961 tgtacgcggc actgctggtg atggcgctgt accagcgcta cctgcgccag gaaaatgcac
     1021 tggtgcgcta tctgtccaac agttcctact ggctgtatct gattcacttc ccgctggcca
     1081 ttgcattgcc gcccctgctg cacgactggc cgctggcacc gacgctgaaa ttcggactgg
     1141 tgttggtcgc caccctgggg ctgggcctgc tgagctatga gtttctggtg cgcgacaccc
     1201 ctgtaggtgc attgctgaat ggcaggcgga ttccgcgccg gcgatcgact tcgctggcgc
     1261 ccgcgccctg gtgacccgct ttttgcacca actacgtcat tccgcgccga ttttgcacca
     1321 caatatggca ctacggcacg tggcacccgc gcagggaacc cacattcagt ggcttttacc
     1381 agcttggcac cgcaattgct tcgtataaaa tgcagaggaa tgcaatggca tggttgcccg
     1441 ctcactgcgg atgcgctatg agacattgaa ggtactcctg aacacataca gattgacaat
     1501 ggacagctag ggttccggtc gcgcgggttt gcgtgatgtc tggtccgaga gctggcgacc
     1561 ttcccagggg aaggttacac ggcgggataa aagcccggga ggtgacactg gcaggtatgc
     1621 cattgattcc tccatccatt caacgtgtaa ccgtgggagc tccaccatgc gcaatctgtt
     1681 aaaggccctt atcctcgctc cgtttattcg taccgttcta acagtattct gtgccgctct
     1741 tgcagttgtt gggatcgcac atgctgaaga aagaaaatcc ttcagtgtgt gttggtccat
     1801 ttatgtgggc tggatgccat gggattacgg cgcccaacag ggcatcgtca agaaatgggc
     1861 cgataaatac ggcatcgcca tcgacgtggt acagatcaac gattacgtcg aatccatcaa
     1921 tcaatatacc gctggcaaat acgatggctg caccatgacc aacatggatg cgctgaccat
     1981 tcctgccgcc ggcggcgtcg attccaccgc cctgatcgtg ggcgatttct ccaacggcaa
     2041 tgacggcgtg gtgctgaaag gcaaaaaatc cctcgctgat attaaaggcc agtccgtcaa
     2101 tctggtggag ctgagcgttt cccattatct gctggcgcgc ggccttgaaa ccgttggtct
     2161 gagcgaaaaa gacctgaaag tggtgaacac ctccgacgcc gacctggtgg ctgcgtttgc
     2221 cacgccggat gtcacctctg tcgtcacctg gaatccgctg ctgtcggaaa tcctgaccat
     2281 gccgaacagc aacaaagtat tcgattcctc gcagattccc ggtgaaatca tcgacctgat
     2341 ggtggtgaac agcgacgtgc tcaaggccaa tccgaaattc ggcaaggcac tggtcggcgc
     2401 ctggtatgaa atcatggcca ccatgagcgc acaggatgcc aaaggcaagg cggcgcggga
     2461 attcatggcc aaggcatcgg gcaccgatct ggccggcttc gaagcgcagc tggcgtccac
     2521 caaaatgttc tacaccgcaa aagacgccgt cgctttcacc accagtgcgc aactgaaaac
     2581 caccatggaa tacgtggcga aattctcgtt cgatcacggc ctgctgggtg acggcgcggc
     2641 ggatgctggc tttatcggag tggaaaccgc cgccggtctg tatggcagca gcaagaatgt
     2701 gaagctgcgc ttcgatccgt cctacatgca gatggcgctc gacgggaaac tgtgaacgga
     2761 aaactgtaaa aacaaaacac tgagccgacg ccatgaaaaa aataatcaac ctgcaaccgc
     2821 acaatggaac gcgctggctg ctggcgttgc tgccgttcct gctgctgttg ctgctgtacg
     2881 tggcggcctc caatgcccgt ctggcggaca atcccggtga caaactgctg cccgccatgg
     2941 gcagcttcgc cgaggcgatc cagcgcatgg cattcgaacc ggacaagcgc agcggcgact
     3001 atctgttgtg gttggatacg ggcagcagtt tgatccggct gggtctgggc gtaggcatca
     3061 gcgcgctggc gggactggtg ttcggaattc tgaacggcgc aataccgttt gcccgcgcgc
     3121 cgctgtcgcc gctggtgacg tttctgtcgc tgattccgcc gatggcgatc ctgccgatcc
     3181 tgttcatcat tttcggcatg gatgaactgg cgaaagtgat gctgatcatc atcggcatca
     3241 cgccggcgct gatccgcgac atgcaacaac gggtactgga aattccagcg gaacagctga
     3301 tcaaggcaca gactctcggc gccagcagct ggcagatcat cctgcgcgtg gtgctcccgc
     3361 agattctgcc gcgcctgctc gcatcggtgc ggctggcact ggggtcggcc tggctgttcc
     3421 tgattgcggc ggaagccatc gcatccaccg atggcctggg ctaccgaatt tttctggtgc
     3481 gccgttatct ggcgatggat gtgattctgc cctacgtgat ctggattacg ctgctggcgt
     3541 ttttcattga ctacgggctg aaaaaaatct cgcagtggag tttcccgtgg ttccacagcc
     3601 agaccggtga cggaggcaag tcgtgagcta tatccagatc cgcaatctgt ggaaggaata
     3661 cgccgatcag gtggtgctgg agcgcatcaa tgcccgcgtc aacaagggcg acttcatcac
     3721 catcgtcggc gcgtccggct gcggcaaaac cacgtttctg aaaatgctgc tgggcacgga
     3781 gtcgccatca cgcggcgcca ttctgctgga agacgcgccg attccggacg aacccagcca
     3841 gcagcgcggc atcgtgttcc agcgctattc ggtgtttccc catctgaccg tgttgaaaaa
     3901 cgtgatgctg gcgcgggagc tggaacacag cccgttgctg ggcaaactgt ttggcagcgc
     3961 ccgccgcacc gccaagcagg aagcactggc gatgctggaa aaagtgggcc tgacggcggc
     4021 catcaacaag tatccgcatg aactttcggg cggcatgcag cagcggctgg cgatcgcgca
     4081 gtcgctgatc cgcaagccgg cgatcctgct gctggatgaa ccgtttggcg cgctggaccc
     4141 cggcatccgc gccgacatgc acaagctggt gctggagttg tggaagcagc atcagctcac
     4201 cattttcatg atcacccatg atctgaagga aggtttttta ctgggcacgc ggctgtgggt
     4261 gttcgacaag ccccgccacg atccccaggc acccggcgcg tttggcgcgc gcataaccta
     4321 cgacattccg gtgggccagt ccgaccggtc tctgtaccag aaaatagatc agcaactgca
     4381 ggtgacgcag gagctggcga tggaaggaaa tgccgcatga cttctgcttt gtttcaggaa
     4441 agcctttacc aataccggat gcccggcgcc gcgcactggt ctctgaaaat gcggcgcggc
     4501 acgattttgc aactgacaga tgtggaaggc ggcgccaatg tgggcctgct gttctacaac
     4561 ccggaggatc tgctggagcg ctacaacgcg ccggacacgc tgaagtgcca gcacacgttc
     4621 aagctcaccg ccggcaattg tctgtattcc gacatgggcc gcattttcgc gtccatcgtg
     4681 gcggatgatt tcggctggca tgagaccgtg tgcggcaaca gcgacgcaaa gaaaacggct
     4741 gcacgctggg gccggcattc ctaccaggat tttcgcaacg agtggtacct gaacggccac
     4801 gacagttttc tggtggagct ggcgaaatac ggcctggggg aaaaggatat ggcggcgaat
     4861 ctgaacctgt tcagcaaggt ggtggtggat gacgccggca atctgagcct ggacgaaggt
     4921 gctgcccgcg ccggtgccag tatcagcctg cggttcgaga tggatacgct ggtgctgctg
     4981 cacacttgtc cacatccgct gaacagggcg agtgaatttc cgcgcaagcc ggtgcaatgt
     5041 gagattttca gggcggcgcc ggtggcggag gatgatttct gccgcaattt ccgcccggag
     5101 aaccagcgcg ggtttgagaa taaccgcttg tatcatctgg gtcgggtgta aggagcctgt
     5161 catgatcaaa gagagcacct tgagtccggc cgatgcaatg cacaaggaga ttgtgcccgc
     5221 cggggattac tggatgcaca agctgaagca cggacagacg ctgcggattc tggatctgga
     5281 agggaatcag gcggcggata cgctgtttta cagccagaag gatccggcgg agcggtacag
     5341 tgcgatggat acgatccggg agcagggcaa tgtgtatctg acggcgggaa cgaagctgtt
     5401 gtcgaatcgc ggtaatctga tgctggagat tacggcggac acctgcggcc ggcatgacac
     5461 gttgggcggt gcctgtgcca ccgagagcaa tacggtgcgt tatgcactgg agaaacgttg
     5521 catgcatgcg tgtcgtgaca gttatctgtt ggcggtggcg cagaatgaga agttcggcat
     5581 gagcaagcgg gatattaccc acaacatcaa tttttttatg aatgtgccgg tgacggcaga
     5641 tggcgggttg acgtttgcgg acgggatttc ggcgccggga aaatatgtgg agatgaaggc
     5701 ggcgatggat gtgattgtgc tgatttccaa ttgtccgcag ctgaataatc cgtgcaatgg
     5761 gtataacccg acgccggttg aagtgattat ctggaattga gtccagttga agctatgtcg
     5821 tgcttcgatt ggtccgcgtc agcatctgag gtgaggttga ttccggacac gccgtgaacc
     5881 catccctggg ggcttctctg cgacatccct gtcgcagaga gtccggaatc aacctcacct
     5941 cagacgttgg cgcttctttg ccaacgcaga ttcttctgga ggacgacctc ggaagaaccg
     6001 aaacacagcg ggacgacccg cacagaaacg caggcacatc atcatgttca acaaagtcct
     6061 gatcgccaac cgtggcgcca tcgccgtccg catcatccgc accctgaa
//
LOCUS       DASUEG010000779         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1224511, whole genome shotgun sequence.
ACCESSION   DASUEG010000779 DASUEG010000000
VERSION     DASUEG010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..400
                     /locus_tag="VFX11_14730"
     CDS             2..400
                     /locus_tag="VFX11_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX4939931.1"
                     /translation="MAIISIIATIAYPSYQNSVKKARRGDAKGAMEGLAQAMERHFTA
                     NNTYLGAGTTGGNTGAPTIYPTQSPIDGGTKFYNLTIQAATATTFTLRATPINGQVGD
                     GMLELDNTGARRWDADNGGTFSATENTWSQ"
     gene            complement(405..1337)
                     /gene="ispH"
                     /locus_tag="VFX11_14735"
                     /gene_synonym="lytB"
     CDS             complement(405..1337)
                     /gene="ispH"
                     /locus_tag="VFX11_14735"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4939932.1"
                     /translation="MEIKMANPRGFCAGVDRAIAIVNRALEVFGAPIYVRHEVVHNKF
                     VVDDLRDRGAVFVDELDEVPDDAIVIFSAHGVSKQVQNEAASRGLKVFDATCPLVTKV
                     HMEVMSYSRDGMECVLIGHEGHPEVEGTMGQYDKSFGGDIYLVETLADVPKLPVRNPA
                     KLAYVTQTTLSVDDTAAIIDALRVQFPQIKGPRKDDICYATQNRQDAVKTLALETDVV
                     FVVGSPNSSNSNRLRELAERCGARAYLVDSPDQLEKAWLDGVRVIGLTAGASAPEELV
                     SQVVNRLKEWGGAVPEELKGREENIVFSMPRELR"
     gene            complement(1403..1885)
                     /locus_tag="VFX11_14740"
     CDS             complement(1403..1885)
                     /locus_tag="VFX11_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4939933.1"
                     /translation="MQSKMNSENRIGQGKRVTLHFSVLLLDGAVVDTTRERAPATFTV
                     GDGNLLPGFEQALFGLKTGDKRSIVLEAANAFGPRNDDNIQIMRRGLFARDMHLAPGV
                     VVSFADKSKAELPGVIVAVDADTVTVDFNHPLAGKDLTFQVEIINVVDADSQTVQLHS
                     "
     gene            complement(1918..2451)
                     /gene="lspA"
                     /locus_tag="VFX11_14745"
     CDS             complement(1918..2451)
                     /gene="lspA"
                     /locus_tag="VFX11_14745"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX4939934.1"
                     /translation="MNEAVKNGADNAQGGATGMLRWLWLSLAVVVLDQITKWVIVFLL
                     EHGEVVPVIPTFNLTHVYNPGAAWSFLANAGGWQRWFFTAVAAVVSVLLVGWLRKIPR
                     TDWWVAMAVALILGGALGNLIDRVLLGHVIDFLDFYWPPADSWSASHFPAFNVADCGI
                     TVGAAMLIIDMIRNPGK"
BASE COUNT          554 a          874 c          690 g          490 t
ORIGIN      
        1 ggtggcgatc atctcgatca tcgccactat cgcctatccg agttaccaga actcggtgaa
       61 gaaagcgcga cgcggcgacg ccaaaggggc catggaaggg ctggcgcagg cgatggagcg
      121 ccacttcacc gccaacaaca cttacctggg tgcgggaacc actgggggca acacaggagc
      181 ccccaccatc tatccgacac agtcaccgat cgatggcgga acaaagttct acaacctcac
      241 cattcaggct gccaccgcca ccacgttcac gctgcgagcg acccccatta atggccaagt
      301 aggagatggc atgctggaac tggacaacac tggcgcccgc cgctgggacg cagacaacgg
      361 cggcactttc agcgcgactg aaaatacctg gagccaatag agactcaacg caactcacgc
      421 ggcatggaga acacgatgtt ctcctcccgc cctttcagtt cctctggcac cgccccgccc
      481 cactccttca ggcggttcac cacctgcgac accagttcct ccggtgcgct ggcgcctgcg
      541 gtcaagccga tcaccctgac gccatccagc caggccttct ccagctgatc ggggctgtcc
      601 accagatagg cgcgggcacc gcagcgttct gccagttccc gtaaccggtt ggaattggaa
      661 ctgttgggcg accccaccac gaacaccaca tcggtttcca gggccagggt tttcaccgcg
      721 tcctgccggt tctgggtggc atagcagata tcgtccttgc gcgggccctt gatctgaggg
      781 aattgcaccc gcagcgcgtc aatgatggcg gccgtatcat ccaccgacag ggtggtctgg
      841 gtcacatacg ccagtttcgc aggattccgc accggcagtt tcggcacgtc ggccaacgtc
      901 tccaccagat agatgtcacc gccgaacgat ttgtcgtact ggcccatggt gccttccact
      961 tccggatggc cctcatggcc aatcagcacg cactccatgc catcgcggga gtaactcatc
     1021 acttccatgt gtaccttggt caccagcgga caggtggcat cgaacacttt aaggccccgg
     1081 ctggccgcct cgttctgcac ctgtttcgac acgccgtggg cgctgaaaat cacaatggcg
     1141 tcgtcgggca cttcgtccag ttcatccacg aacactgcgc cgcgatcacg cagatcgtcc
     1201 accacgaact tgttgtgcac cacttcatgc cgcacataaa taggcgcgcc aaacacttcc
     1261 agcgcccggt ttacaatcgc aatcgcgcga tcgacgccgg cacaaaatcc ccgcggattt
     1321 gccatcttga tttccatgtc tgcctcactc acttcatctt gaagttgttg cgtctgaaaa
     1381 tgtcggctgg ttgcgacgtc cgtcagctgt gcagttgcac agtctgcgaa tcggcatcca
     1441 ccacgttgat gatttccacc tgaaacgtca gatccttgcc cgccagcgga tgattgaaat
     1501 ccacggtgac cgtgtcagcg tctaccgcca cgatcacgcc cggcagctcc gccttgcttt
     1561 tgtccgcgaa tgacaccaca acgcccggtg caaggtgcat gtcgcgggcg aacaggccac
     1621 gccgcatgat ctggatattg tcgtcattgc gcggcccgaa cgcattggcc gcctccagca
     1681 cgatggaacg cttgtcgccc gtcttcaacc cgaacagggc ctgctcgaag cccggcagca
     1741 gattgccatc gcccacagtg aaggtagcgg gtgcgcgctc gcgggttgta tccaccacgg
     1801 caccatccag cagcaggact gaaaaatgca gggtcacacg cttgccctgt ccgatccggt
     1861 tttcactgtt catcttggac tgcactcaac taccactatg ctatacacca aaattgccta
     1921 tttgccgggg ttgcggatca tgtcgatgat cagcatggcc gcgcccaccg tgatgccgca
     1981 atcggcgaca ttgaaggcgg gaaaatgact ggcactccag ctatcggcgg gcggccagta
     2041 gaaatccaga aaatcgatga catggccaag gagcacgcgg tcgatcagat tgcccagcgc
     2101 gccgcccaga atcagggcca ctgccatcgc cacccaccag tcggtgcggg gaatcttgcg
     2161 cagccagccc accagcagaa cgctcaccac cgccgccacg gcagtgaaaa accagcgctg
     2221 ccagccaccg gcattggcca gaaaactcca ggccgcgccg ggattgtaaa catgggtcag
     2281 attgaacgtg ggaataaccg gaaccacttc gccgtgctcc agcagaaaca cgatcaccca
     2341 cttggtaatc tggtccagca cgaccaccgc cagcgacagc cacaaccacc tcagcatgcc
     2401 ggttgcgccg ccctgcgcgt tgtccgcacc gttcttgacg gcttcattca tcggagtacc
     2461 tttgctggtg gccatttagc agaattccct ggcttcgccg tcgccttcca cgttgtcgac
     2521 acaacgcaaa cagatttccg gatgcgccgc attggcaccc acatcggcgc gatgatgcca
     2581 gcaacgcacg cacttggtat cggcgctg
//
LOCUS       DASUEG010000780         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_719390, whole genome shotgun sequence.
ACCESSION   DASUEG010000780 DASUEG010000000
VERSION     DASUEG010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719390"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3436)
                     /locus_tag="VFX11_14750"
     CDS             complement(<1..3436)
                     /locus_tag="VFX11_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4939935.1"
                     /translation="MSSSRNRVMLTTALVLSMLGALLFFVSGWMEDVFRRELKAQLLT
                     ARNQIHHDLSGWLEGRQQILQRWVRDDLLVARIRQLPPGGDYELNAGLQTALAITDQL
                     APLLDNSEHLSFAVLDGAGKRLVSSLTAELGMTDPLALQPDLLRRLFSGEPVYYFAQE
                     TLLNDAGTTYEAGGVVHLLQPIFHGNEVIAALKLSVNLEREMQRMFRLDHLWEHSIVY
                     AFDERGNLIVQHPRSRSDIPDTRAAEPVLAYLALLRGESSDDAGGRINLDGYHGLAAQ
                     DVAGAWAWYNRLHLGIAVEVDRADAFRFPAIFNLFLGLVTLLAAAVVILLGWLYYRRG
                     SMLSDEMRLMESLTEGTSSLVLVTGLDGRIRRANKAAMTLFRRNDLVGKTYSDLMPVD
                     TAVQWQTHDSVVLQSQQAQEFNEVLNLDGSRRWLQLSRFPLRNFRQKVVGIGCVGTDV
                     TERRKTEEALEHYKRNLDDIVLQRTQAVEQERRQLDDIVTHALDAIITISANGTIETF
                     NPAAERMFGYSHEEAIGQNVSLLMEDDLATQHGSFLRKYSEVQPARPSVVVGRGRQIL
                     ARSRDKRLFPIEISVARVGSGNQLRFIGVIRNLTERSEQDEILKVLFEHSTDAHLLFV
                     DGKVLNCNFAAMKMLDAKSKAAVIGASMADFSPYRQPDGGVSGEKYQALERYARDKGG
                     ERFDWLLQRSGGDSLLVEVNLTPVVVGEKKSMLAVWHDISERIAAQNTIKRSEQRIRD
                     IIDSTIQLMLLLAPDGTLLEGNKAAFSLIGVSPDDVLGRKFWDTPWWSHSRPLQIQMR
                     ERVLNVRDGRTERFDALQRTPDGRTLQLDFSMTPIMANDDVELIVVEGHDVTAILEAR
                     EAERQAREEAESANRAKSEFLARMSHEIRTPMNAIIGMTRLCLNTSLTERQRQYLDSV
                     DNAANSLMGIINDILDFSKIEAGKLELELIPFSLRDIFANVGAVIGIKAQEKGLEFVI
                     SDIDVPQQLMGDPMRLQQVLLNLCSNGVKFTEKGHVSLEVTPVAMSSRQVRLRFQVQD
                     TGIGLSPDQQARLFESFTQADPSISRKFGGTGLGLTICRQLVNAMGGEITVHSAPGAG
                     SVFAFELPFQTLAGRSTQGSRNRRARSNGEQILVVDDNPVCWKIAEKILLNAGYRVL"
BASE COUNT          689 a         1127 c         1024 g          708 t
ORIGIN      
        1 ccagcacccg gtagcctgcg ttgagcagga tcttctctgc gattttccag cacaccgggt
       61 tgtcatccac caccaggatc tgttcgccgt tgctgcgtgc acgccggttg cgggaaccct
      121 gggtgctgcg tcccgccagg gtctggaacg gcagctcgaa cgcaaatacg ctgcccgcgc
      181 cgggcgcact gtgcaccgtg atttccccgc ccatggcgtt gaccagctgg cggcagatag
      241 taagaccgag cccggtacca ccgaatttgc ggctgatgga cgggtcggcc tgggtgaagg
      301 attcaaacag acgtgcctgc tggtcggggc tgaggcctat accggtatcc tgcacctgaa
      361 aacgcagtcg tacctggcgg ctggacatgg ccaccggtgt cacttccagg ctgacgtggc
      421 ccttctcggt gaacttgacg ccgttggaac acaggttcag caacacctgc tgcaggcgca
      481 tggggtcgcc catcagctgc tgcggcacat caatgtcgct gatgacgaat tccagaccct
      541 tctcctgcgc cttgataccg atcaccgcac caacattggc gaaaatatcg cgcaggctga
      601 aaggaatcag ttccagttcc agcttgcctg cttcaatctt cgagaaatcg agaatatcgt
      661 tgatgatccc catcagcgaa ttggcagcgt tgtccaccga atccagatac tgccgctgcc
      721 gttccgtcag actggtgttc aggcacagcc gggtcatacc gatgatggca ttcatgggcg
      781 tgcggatttc gtggctcatg cgggcaagaa attcgctctt ggcgcggttg gcggattccg
      841 cttcctcgcg cgcctgccgc tcggcctccc gcgcttccag aatggcggtg acgtcgtgcc
      901 cttccaccac gatcagttcc acgtcgtcgt tggccatgat gggcgtcatg gagaaatcga
      961 gctgcagggt gcggccgtcg ggtgtgcgct gcaaagcgtc gaaacgctcg gtgcgaccat
     1021 cgcgcacgtt gagcacgcgc tcccgcatct ggatctgcag tggacgcgag tgactccacc
     1081 agggtgtgtc ccagaacttg cggcccagca catcgtccgg actcacgcca atcaggctga
     1141 aggccgcctt gttgccttcc agcaaggtac cgtccggcgc cagcagcagc atcagctgaa
     1201 tggtactgtc gatgatgtca cggatgcgct gctcgctgcg cttgatggtg ttctgcgcgg
     1261 cgatgcgttc gctgatgtcg tgccacaccg ccagcatgct tttcttttca cccaccacta
     1321 ccggcgtgag attcacctcc accagcaggc tgtcgccgcc gctgcgctgc aacagccagt
     1381 cgaaccgttc accccccttg tcgcgggcgt agcgttccag tgcctggtat ttttcgccgg
     1441 agacgcctcc gtccggttgc cggtagggcg aaaaatccgc catgctggcg ccgatcacgg
     1501 cagctttgga ttttgcgtcc agcattttca tcgcggcaaa attgcagttc agcactttgc
     1561 catcgacaaa caaaagatgc gcatcggtgg aatgctcaaa cagcaccttg agaatttcgt
     1621 cctgctcgct gcgctcggtg agattgcgga tgacaccgat gaaacgcaat tgattgccac
     1681 tgcccacccg tgccactgaa atctcgatgg ggaacaggcg tttgtcgcgg gagcgggcca
     1741 ggatctgccg gccacggccc accaccacgg acggccgcgc cggctggacc tcgctgtatt
     1801 tgcgcaggaa actgccgtgc tgcgtggcga ggtcgtcttc catcagcaac gagacgttct
     1861 gtccaatcgc ctcctcgtgg ctgtagccaa acatgcgctc tgccgccgga ttgaaggttt
     1921 caatagtgcc gttggcgctg atcgtgataa tggcgtccag ggcgtgggtg acaatatcat
     1981 ccagctgccg ccgttcctgt tcgaccgcct gcgtgcgctg caacacgata tcgtccagat
     2041 tgcgcttgta gtgctccagc gcctcctcgg tcttgcgacg ctctgtaacg tcggtaccta
     2101 cacagccgat tcccaccact ttctgccgga aattacgcag cgggaaacgc gacaactgca
     2161 gccagcgccg gctgccatca agattcagca cttcattgaa ttcctgcgcc tgctgcgact
     2221 gaagcactac ggagtcatgg gtctgccact gcaccgcggt gtcgacaggc atcaggtcgg
     2281 aataggtttt gcccaccaga tcattacggc ggaacagggt catggccgcc ttgttggcgc
     2341 gccggatgcg gccgtcaagg cccgtcacca gcaccagtga agacgtgccc tcggtgagcg
     2401 attccattag tcgcatttcg tcactgagca tgctgccgcg gcggtaatac agccagccca
     2461 gcagaatcac caccgccgcc gctagcaggg tgaccagccc caggaacaga ttgaaaatgg
     2521 caggaaaacg gaaggcgtcg gcgcggtcca cttccaccgc aatgcccagg tgcagccggt
     2581 tgtaccaggc ccatgcgccc gccacgtcct gcgctgccag tccatgataa ccgtcgagat
     2641 tgattctgcc gccggcatcg tcactggatt cgccccgcag cagcgccaga taggccagca
     2701 caggctcggc ggcccgggta tccggtatat cactgcggga acgcgggtgc tggacgatca
     2761 gattgccacg ctcgtcgaag gcatagacga tgctgtgttc ccacaggtgg tcgagacgga
     2821 acatgcgttg catttcccgc tcaaggttca cgctgagctt gagtgcggca atcacttcat
     2881 tgccatggaa aatcggctgc agcaaatgca caaccccacc ggcctcatac gtggtgccag
     2941 cgtcgttgag caatgtttcc tgggcaaagt agtaaacggg ttccccgctg aacagccggc
     3001 gcaacaggtc aggctgcagt gccagcggat cggtcatccc cagttccgcc gtaagcgacg
     3061 acaccagccg ttttccagca ccatccagca cggcgaagct aaggtgctca ctgttatcca
     3121 gcaggggtgc cagctggtcg gtgatcgcca gcgccgtttg cagaccggca ttgagttcgt
     3181 aatcgcctcc cggcggcaac tgccggattc gcgccaccag caggtcatcc cgcacccagc
     3241 gctgcaaaat ctgctggcgg ccttccagcc agcctgacag gtcatgatgg atctggttgc
     3301 gcgccgtgag cagctgggct ttcagttcac gccgaaacac atcctccatc cagccggaca
     3361 caaaaaacag cagggcaccg agcatcgaca ataccaatgc ggttgtcagc atgacgcgat
     3421 ttcggctgga cgacataagc tacccggaac gacactccct gaactttgag cgtagcatcg
     3481 attgaccgga ttctctggct ccggcgcggg cagcaaaccg ctccaccccg ctgcaccgcc
     3541 ttgacgcg
//
LOCUS       DASUEG010000781         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2174493, whole genome shotgun sequence.
ACCESSION   DASUEG010000781 DASUEG010000000
VERSION     DASUEG010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174493"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VFX11_14755"
     CDS             complement(<1..1102)
                     /locus_tag="VFX11_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX4939936.1"
                     /translation="MKTSLKTVLFASVLMAGTAAAVGLHPQWSKPANPEPAVKPGGTQ
                     SVVAPTVATPKIEVVFVLDTTGSMGGLIDAAKNNIWSIASSMASAKPAPEIRMGLVAY
                     RDRGDAYVTKVLDLSGDMDSMYAALMDFRADGGGDGPESVNQALHDAVHRIGWSEDSN
                     SYKVIFLVGDAAPHMDYANDVPYPETLKIAAQKGIVVNCIQAGAESDTRQVWQHIASL
                     NQGRYFQVEQSGSAVAVTTPYDDKIAALSKQLDETRVFYGDADTRRALEKKTDAAKKV
                     YAAAPAAVQAKRAEFNASAAGADNLFADSELVDDVASGRVKLEEVDKARLPEPLQRLS
                     KDEQAHLISTKATQRKQLQAEIAQLGKQRQDYI"
     gene            complement(1133..1699)
                     /locus_tag="VFX11_14760"
     CDS             complement(1133..1699)
                     /locus_tag="VFX11_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939937.1"
                     /translation="MKALNFSSGVMMALVLSVSGAVLLSGTTLLTGFSIALRAVLLLI
                     MLTYWLWLLRVTGARAGISLMAAGWLLLGVTLVALNPPFVVWLATCTGWLWLQRSALR
                     YCRPGPALADAGLNLLALCAAVVAYWHSGSFLLACWSFFLLQAACVFIPAQAIAGTST
                     GATDSNRFAQARHSAEQALRRLRQSESI"
     gene            complement(1696..2220)
                     /locus_tag="VFX11_14765"
     CDS             complement(1696..2220)
                     /locus_tag="VFX11_14765"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX4939938.1"
                     /translation="MTVLTRLSRLFRADLHALLDRMEAPDVLLQQAIRDMEGEIQLQQ
                     QEQKKRALEQQQLQRRCSDLQERLQQQETELDLCLDAGNDALTRALLRRRLEQEQTLR
                     LLQQRLQTLTEKMRGADTELQQQQRELKALREQAEVLLQTPSFTRDAADACAVTEADV
                     ELALLKAKSRRSPA"
BASE COUNT          396 a          701 c          690 g          467 t
ORIGIN      
        1 cgatatagtc ctgccgctgt ttgcccagtt gcgcgatttc cgcctgcagc tgtttgcgct
       61 gagtggcctt ggtgctgatc aggtgcgcct gctcatcctt gctcaggcgt tgcagcggtt
      121 ccggtaaacg ggctttgtcc acttcctcca gtttcacgcg gccactggcc acatcatcca
      181 ccagttcgct gtcggcaaag aggttgtcag caccggcagc tgaggcgttg aattccgcgc
      241 gcttggcctg cacggcagca ggtgccgcgg catagacttt tttcgcggcg tcggtttttt
      301 tctccagtgc acgccgggtg tcggcgtcgc cataaaacac gcgggtttca tccagctgct
      361 tcgagagcgc ggcgatttta tcgtcatagg gcgtggtaac ggccaccgcg ctgccacttt
      421 gctccacctg gaaataccgg ccctggttga gactggcaat gtgctgccac acctggcgcg
      481 tgtcggattc ggcgccggcc tggatgcagt tcacaacgat gcctttctgc gccgcgattt
      541 tcagcgtttc cggatagggc acgtcgttgg catagtccat gtgcggcgcg gcgtcaccca
      601 ccaggaaaat caccttgtag ctgttgctgt cctcgctcca gccaatgcgg tgcacggcat
      661 catgcaatgc ctggttgacg ctttcggggc cgtcgccacc gccgtcggcg cggaagtcca
      721 tcaatgcggc gtacatggaa tccatgtccc ccgacagatc cagcactttt gtcacatagg
      781 catcgccgcg atcacggtaa gccaccagcc ccatccggat ttccggtgcc ggtttggcgg
      841 aggccatgct gctggcgatg gaccagatgt tgtttttcgc ggcgtcgatc aggccgccca
      901 tgctgccggt ggtatcgagc acgaatacca cttcgatttt cggtgtggca acggtgggcg
      961 cgacaaccga ctgcgtgccg ccgggtttta cggcgggctc gggattggca ggtttcgacc
     1021 actgcggatg cagaccgaca gcggcggcag tgccggccat cagaacagaa gcaaacagta
     1081 cggttttcag cgatgttttc atggcggatc tcctgggtgg ttccgggtga attcagatgg
     1141 attcggattg gcgcagacgg cgcagggcct gttccgcgct atggcgggcc tgggcgaaac
     1201 ggttgctgtc ggtggcaccc gttgatgtcc ctgcgattgc ttgcgcggga atgaagacgc
     1261 aggcagcctg cagcaggaaa aaactccagc acgccagcag gaaactgccg ctgtgccagt
     1321 aagcgacaac ggctgcgcac agggccagca ggttcaggcc ggcgtccgcc agggcgggcc
     1381 cggggcggca ataacgcaat gcactgcgct gcagccacaa ccagccggtg caggttgcca
     1441 gccagaccac aaacggcgga ttgagcgcaa ccaacgtgac gcccagcagc aaccagccgg
     1501 cggccatcag gctgatgccg gcacgggcgc cggttacccg cagcagccag agccagtacg
     1561 tcagcatgat cagcagcagc accgcgcgca gggcaatact gaatcccgtc aatagagtgg
     1621 tgccggacaa cagcaccgca ccactcacgc tcagcaccag cgccatcatc acgccgctgg
     1681 aaaaattcag cgctttcatg cgggactcct tctgcttttg gctttcagca gtgccagttc
     1741 cacatcggct tcggtgacgg cgcaggcatc ggcagcatcg cgggtgaacg acggcgtctg
     1801 caacaacacc tctgcctgtt cccgcagcgc cttcaattcc cgctgctgct gttgcagttc
     1861 agtgtcggcg ccgcgcattt tttccgtgag cgtttgcagg cgctgttgca gcagacgcag
     1921 tgtctgttcc tgttccagcc ggcgccgcaa cagtgcgcgg gtcagcgcat cgttgccggc
     1981 gtccagacac aggtccagct ccgtttcctg ttgctgcaaa cgctcctgca aatcactgca
     2041 gcgccgctgc aactgctgtt gttccaacgc gcgctttttc tgctcctgct gctgcagctg
     2101 gatttcacct tccatgtcgc ggatggcctg ctgcaacagg acgtccggcg cttccatgcg
     2161 atccagcagc gcatgcagat cggcgcggaa caggcgggat agtcgggtca gtacggtcat
     2221 gaggtgtcct ccttcggtga gtcaggatca gtct
//
LOCUS       DASUEG010000782         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_525379, whole genome shotgun sequence.
ACCESSION   DASUEG010000782 DASUEG010000000
VERSION     DASUEG010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525379"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFX11_14770"
     CDS             <1..372
                     /locus_tag="VFX11_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008736091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4939939.1"
                     /translation="DQANLSFRDTIVPRIGVDFRVNDTLTVRAGVAMEDAVLDSDRSL
                     DVNYLDNDRLVVGLGASAEFRDPWIFAFPVRVDVGYQMHVLKDRKFDLTSSQVNNGQP
                     YETLETSGDVHVFAGSLTLKF"
     gene            386..>2703
                     /locus_tag="VFX11_14775"
     CDS             386..>2703
                     /locus_tag="VFX11_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4939940.1"
                     /translation="MRIFLLLPLLAVLLAGCDNDPQRVREPAAFSGSAGLLYTYPFNG
                     QTRIAPTAPVVLQFATTLSVTGGTLAGAFRFESAAGQPVPFTLSTTNNGRGVYLLPNA
                     PLAENTEYRVSWVGLKGSDGPLATVPLSFTTRAANTGADSQTRSNTTFQVERSLPVQS
                     NFPFMDFSTIRLQFTQPVESRTAAYGTSVRLEDAGGNLVPARLLLSRHLMSIDPVNLL
                     TPGQTYTLRLTSAIESTHGQVLVPGAYAALNLTPANSAPRETLVMQVPSTPVASPLTG
                     KTINNVPITSRLLGNDSASQQGGNLYTELAFVPNYPTATPLRVPRGNLLTGTAVDVQI
                     VGRVPAGLNTGDISVNIVSDANGYMTENPYSTAVDAPRIVYLTMDAAMSSTTPSANGA
                     FNQNLMHIDVVGMAIVKEGRLVIDAVGVAELEVLGLDQAAGVLSLHLEGYADQTTAPA
                     QPVDVTPPALQSWAPGDEDNRARPGDPIILTFTEPLDPNSLTTDSIVLNRNGVAQPID
                     LRMDGSSIVIRPQQALTHNADYNVLVGPEVTDIAGNATTPLSLDFRLPDLVGPASRSP
                     LVLAGYPGYPCVTSGRNATANEQGRCLDGKGTDDILPIPQMPADRIIQVQFSQDINPA
                     SVVLGAACGSGTFRVEQVSSGGTCLGTVAGRLEKGSSSLRFVPDQPWVAGQYYRYMLM
                     SNGNSQSSTINCNGTQGICGSNNLPLQTQTLAQTPSAAPTATGGGLPMEIWFRGTAAS
                     TTVYQRLRALPATDVNVNFLHDTPDEIGAVDTG"
BASE COUNT          517 a          933 c          788 g          465 t
ORIGIN      
        1 gatcaggcca acctgagctt ccgtgacacc attgtgcccc gcatcggcgt ggacttccgc
       61 gtcaatgaca cgctcacggt acgtgccggt gtggccatgg aggatgccgt gctcgactcc
      121 gaccgctcgc tggacgtgaa ctatctcgac aacgaccgtc tggtggtggg cctgggcgcc
      181 agcgccgagt tccgtgatcc gtggatcttt gccttcccgg tacgggtgga tgtcggctac
      241 cagatgcatg tgctcaagga ccgcaagttc gacctgacct ccagccaggt caataacggg
      301 cagccctatg aaaccctgga aaccagcggc gatgtgcatg tctttgccgg ctcgctgacg
      361 ctgaaattct gaggaggacg ccatcatgcg cattttcctc ctgttacccc tcctggccgt
      421 cctgctggcc ggctgcgaca acgatcccca gcgggtgagg gaaccggccg cctttagtgg
      481 ctccgccggc ctgctctaca cctacccctt caatggccag acccgcatcg cccccaccgc
      541 gccggtggtc ctgcagtttg caaccacact gtcggtcacc ggcggaacac tcgccggcgc
      601 cttcaggttc gaaagcgctg ccgggcagcc ggtgcccttc accctctcga cgaccaataa
      661 cggccgaggg gtttacctgc tgccaaacgc cccgcttgcc gagaacaccg aatatcgtgt
      721 gagctgggtc ggcctgaaag gcagtgacgg cccgctggcg actgttccgc tgagctttac
      781 cacgcgcgcc gccaacaccg gtgcagacag ccagacgcgg agcaacacca ccttccaggt
      841 agaacgctca ttgcccgtac agagcaattt cccgttcatg gatttctcga ccatccgcct
      901 gcaattcacc cagccggtgg agtcgcgaac tgccgcatat ggcaccagcg tgcgactgga
      961 ggatgccggc ggcaatctgg tgccggcacg cctgctgctc agtcgccacc tgatgagcat
     1021 tgatccggtc aacctgctca cgcccggcca gacctatacc ttgcgcctga ccagtgccat
     1081 cgaaagcact catggccagg tactggtgcc gggcgcttat gcagcactta accttacccc
     1141 ggccaattcc gcaccgcgtg aaacactggt gatgcaggta ccgtcgacgc cggtggcctc
     1201 gccacttacc ggcaaaacca tcaacaacgt accgatcact tcgcgtctgc tgggcaatga
     1261 cagcgcctcg caacaaggcg gcaacctgta taccgaactg gccttcgttc ccaactaccc
     1321 gaccgccact ccgctgcgcg tgccgcgcgg caacctgctg acgggtacgg ctgtggacgt
     1381 gcagatcgtc gggcgcgtac cggccggcct caataccggc gacatcagcg tcaacatcgt
     1441 gtccgatgcc aacggctaca tgacggaaaa tccctacagc acggccgttg atgccccgcg
     1501 catcgtttac ctgaccatgg atgcggccat gtcttcgacc accccgtccg ccaacggcgc
     1561 cttcaaccag aacctcatgc acatcgatgt cgtgggcatg gccatcgtga aggaaggccg
     1621 gctggtgatt gatgccgtcg gcgtggccga actggaggtg ctgggactgg atcaggcggc
     1681 cggcgtgctg tccttgcatc tggaaggcta tgccgaccag accacggcac cggcccagcc
     1741 ggtcgacgtg acgcccccgg cgctgcagtc ctgggcaccg ggcgacgaag acaatcgcgc
     1801 caggccaggt gacccgatca tcctgacgtt taccgagccg ctggatccga acagcctgac
     1861 caccgacagc atcgtgctca accgcaacgg tgtcgctcag cccatcgatc tacgcatgga
     1921 cggcagctcc atcgttatcc gtccgcaaca ggcgctgacg cacaacgccg attacaacgt
     1981 gctggtgggc cccgaggtca ccgacatcgc tggcaatgca acaacccccc tcagcctgga
     2041 cttccggtta ccggacctgg tcggtcccgc cagccgttcg ccgctggtac tggccggcta
     2101 cccgggctat ccctgcgtca ccagtggtcg caacgcaacc gcgaacgagc aaggccgctg
     2161 ccttgatggc aaggggacgg acgacatcct gcccatccca cagatgccgg ccgaccgcat
     2221 catccaggtg cagttctcgc aggacatcaa tccggccagc gtggtgctgg gcgccgcttg
     2281 cggtagcggc accttccgcg tggagcaggt gagtagcggg ggcacatgcc tgggcaccgt
     2341 ggccggacgg ctggaaaaag gcagctcgtc gttgcgcttt gttccggacc agccctgggt
     2401 ggcgggacag tattaccgct acatgctgat gtccaacggc aacagccagt cgtccaccat
     2461 caactgcaac ggcacgcagg gcatttgcgg tagcaacaac ctgccattgc agacgcaaac
     2521 gctagcgcag acacccagcg cggcacccac cgccacgggc ggcggattgc cgatggaaat
     2581 ctggttccgc ggcaccgccg cgtcgaccac ggtgtaccag cgcctgcgtg cactgccagc
     2641 gacggacgtc aacgtaaact tcctgcacga cacaccggat gaaatcggtg cggtcgacac
     2701 agg
//
LOCUS       DASUEG010000783         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_142271, whole genome shotgun sequence.
ACCESSION   DASUEG010000783 DASUEG010000000
VERSION     DASUEG010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142271"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFX11_14780"
     CDS             complement(<1..310)
                     /locus_tag="VFX11_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX4939941.1"
                     /translation="MTSQKHLYLHNRNRLRDVSSFDPDLRRFERLWIDHVLASDASAM
                     SAVTTCDQLLDRIDQRVQWEAQHETEESRYVREAMTRPEFRILVQEFALDGLTEAQSF
                     Y"
     gene            complement(279..1109)
                     /locus_tag="VFX11_14785"
     CDS             complement(279..1109)
                     /locus_tag="VFX11_14785"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4939942.1"
                     /translation="MSDKDVFLPLVTPFNDAGRVCRDSVARLMRFARPNVDGFVPCLT
                     SGEGWLLSDAQWHDMVQFCVQFADGLPVIAGIERATTEAVIERAHVALQLGAQGVICT
                     APFQPGLSQNAIYDHFVRVHDATPLDLYIYFESTLCNNQWNINTLLSLCALPRVKAIK
                     ESSGENKVAPHLRNIRRMGVKVYQGWEDKILEYDADGSMVSYCNLNPRLCQEVTRQRS
                     YAANALMNEQCRNKGVFLPDWYRHLKRDLHARGIIHSPRTLEPAKDIPHDQPEASLPA
                     "
     gene            complement(1102..1620)
                     /locus_tag="VFX11_14790"
     CDS             complement(1102..1620)
                     /locus_tag="VFX11_14790"
                     /inference="COORDINATES: protein motif:HMM:NF039371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6190 family protein"
                     /protein_id="HEX4939943.1"
                     /translation="MNSSETGPHFVDATFFLGMHDSDDQRRMQSLALFTHCLTTHLFM
                     NLEQVGLCDDVIWCRSRQEQDAYYPFMDNLHTLLQIQRIGFTRDDLTRALRDPQLRQL
                     PLQQACTLSQVMNRHGRLHTHDPLLWQDAALQPWLAPPLHQPEQPFPAPLDSLYQDSL
                     HLTLNLQESRYV"
     gene            complement(1607..2797)
                     /locus_tag="VFX11_14795"
     CDS             complement(1607..2797)
                     /locus_tag="VFX11_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4939944.1"
                     /translation="MPASTALTDASRVSHKEDITQHEIDALKTEFNLADAHTHQKQSP
                     SQRDIVASLPDIWYQSEQRTQFEAERAFIEAFYTLHGQHTALERIDHIYLVYAASIGM
                     QITATYLMKHRMRVGLIEPCFDNLHDLMKHAQIPLFPLDESLFHDASTVYDNLMKNAL
                     TLDAIVLVDPNNPTGFSQFARDSKPFLEVVRFCCDFNKILILDFSFASFMLASGGRRP
                     DVYRILENSGVRYIAMEDTGKTWPLQDAKCATILTSRDLNDEIYNIVTSVLLNVSPFV
                     LNIVTRYVLDSQQDGFASVGDLLQRNRVRAIEHLDKGILRYQTPLIRTSVAWFEIADP
                     ELSADQLQHCLLQSRIYVLPGKYFFWKHPARGQRFIRVALARDETLFQNAMYAMEDAL
                     HELQ"
BASE COUNT          529 a          822 c          931 g          593 t
ORIGIN      
        1 agtagaacga ttgcgcttcc gtgagcccgt ccagcgcaaa ttcctgcacc agaatgcgga
       61 actccggccg tgtcatcgcc tcgcgcacgt aacgtgattc ttccgtttca tgctgcgcct
      121 cccactgcac acgctggtcg atgcggtcca gtagctggtc gcaggtggtg acggcgctca
      181 tggcgctggc atcgctggcc agcacgtggt cgatccacag gcgttcaaac cggcgcaggt
      241 cgggatcgaa gctgctgaca tcgcgcagcc ggttgcggtt atgcaggtag agatgcttct
      301 ggctggtcat gagggatgtc cttggcgggt tcaagggtac ggggggaatg gatgatgccg
      361 cgcgcgtgca ggtcccgttt caggtggcgg taccagtccg gcagaaacac gcccttgttg
      421 cgacactgct cgttcatcag cgcattggcc gcatagctgc gctgccgggt cacttcctgg
      481 cacaggcgcg gattcagatt gcagtacgac accatggaac cgtcggcgtc gtattccagg
      541 atcttgtcct cccaaccctg gtacaccttc acccccatgc ggcggatatt gcgcaagtgt
      601 ggcgccacct tgttttcgcc gctggattcc ttgatggcct tcacccgtgg cagcgcgcac
      661 agcgacagca gggtgttgat gttccattgg ttgttgcaca aggtggattc gaaatagatg
      721 tagagatcca gcggcgtggc gtcgtgcacg cgcacgaagt gatcgtagat cgcgttctgc
      781 gacaggcccg gctggaacgg ggccgtgcag atcacgccct gggcacccag ttgcagggct
      841 acgtgggcgc gctcgatcac ggcctcggtg gtggcgcgct cgatgcccgc gatcaccggc
      901 aggccatccg cgaattgcac gcagaattgc accatgtcgt gccactgcgc gtccgacagc
      961 agccagcctt ctcccgaggt gaggcagggt acaaatccgt ccacgttggg ccgggcgaaa
     1021 cgcatcaatc gcgccacgct gtcacggcac acacggccgg catcgttgaa cggcgtgacc
     1081 agcggcagaa acacatcctt gtcagacata acggctctcc tgcaggttga gggtgagatg
     1141 cagcgaatcc tgatacaggc tgtccagcgg cgcgggaaac ggctgctccg gctggtgcag
     1201 cggcggcgcc agccagggtt gcagtgccgc gtcctgccac agcagcggat cgtgggtgtg
     1261 cagcctgccg tgacggttca tcacctggct cagggtgcag gcctgctgca gcggcagctg
     1321 acgcagttgc ggatcgcgca acgcccgcgt cagatcgtcg cgggtgaagc cgatgcgctg
     1381 gatttgcagc agcgtgtgca gattgtccat gaacggatag taggcatcct gttcctgccg
     1441 gctgcgacac cagatcacat cgtcacacag ccccacctgt tccagattca tgaacaggtg
     1501 ggtggtcagg cagtgcgtga acagcgccag cgattgcatg cgccgctggt cgtcgctgtc
     1561 gtgcatgccc agaaagaacg tggcatccac aaagtgcgga ccggtttcac tggagttcat
     1621 gcagtgcgtc ctccatggcg tacatcgcgt tctggaacag ggtttcgtcg cgggccagtg
     1681 ccacccggat gaaacgctgg ccccgcgccg ggtgcttcca gaaaaaatat ttgcccggca
     1741 gcacgtagat gcgcgattgc agcaggcagt gttgcagctg gtcggccgac agttcaggat
     1801 cggcgatttc gaaccaggcg acactggtgc ggatcagcgg cgtctgataa cgcaggatgc
     1861 ccttgtcgag gtgctcgatg gcccgcacgc gattgcgctg cagcagatcg cccaccgatg
     1921 caaaaccgtc ctgctggctg tccagcacat aacgggtgac gatgttgagc acgaacggcg
     1981 acacgttcag cagcacgctg gtgacaatgt tgtagatctc gtcattcaga tcgcggctgg
     2041 tgaggatggt ggcgcacttg gcgtcctgca gtggccaggt cttgccggtg tcttccatgg
     2101 caatgtagcg cacgccggaa ttttccagga tgcggtagac gtcaggccgc cgtccaccgg
     2161 atgccagcat gaacgacgca aaggagaaat cgagaatcag gatcttgttg aaatcgcagc
     2221 agaagcgcac tacttccaga aagggcttgc tgtcgcgggc gaactggctg aagccggtgg
     2281 ggttgttcgg gtccaccagc acgatcgcgt ccagcgtcag cgcgtttttc atcaggttgt
     2341 cgtacaccgt ggacgcgtcg tgaaacagcg attcatccaa ggggaacagt ggaatctgcg
     2401 cgtgcttcat caggtcgtgc agattgtcga agcagggctc gatcaggccc acgcgcatgc
     2461 ggtgtttcat caggtaggtg gcggtgatct gcatgccaat ggacgccgca tacaccagat
     2521 agatatggtc gatccgctcc agcgccgtat gctggccgtg cagggtgtag aacgcctcga
     2581 taaacgcacg ctccgcctcg aactgcgtgc gttgttcgga ctggtaccag atgtccggca
     2641 ggctggcgac gatgtcgcgc tggctcgggc tctgtttctg gtgggtgtgg gcgtcggcca
     2701 ggttgaattc cgttttcagc gcatcaattt catgctgggt gatgtcctcc ttgtgcgaga
     2761 cacgtgaagc gtcggtcagc gcggttgagg ctggcatgtt catgggcgtt tcctttcgtt
     2821 gggggtgtca gggttcgatg atgatttttt ccatcatgcc gtggtcgcca tgatc
//
LOCUS       DASUEG010000784         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_650705, whole genome shotgun sequence.
ACCESSION   DASUEG010000784 DASUEG010000000
VERSION     DASUEG010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650705"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2186)
                     /gene="ileS"
                     /locus_tag="VFX11_14800"
     CDS             complement(<1..>2186)
                     /gene="ileS"
                     /locus_tag="VFX11_14800"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX4939945.1"
                     /translation="GDWQKPYLTMDFKTEADTIRALGKIAQNGHLVKGFKPVYWSVVG
                     GSALAEAEVEYQEKTSTQVDVRFHPVDVAAFAQKFGVSDVTLPLSVVIWTTTPWTLPA
                     NQAVALNRDLPYSLVQMNVGNGDEIILLASGMVESVCARYGVESYRVLGEAQGDTLEH
                     QQLRHPFIDRIVPVVMGEHVTTDAGTGAVHIAPDHGMDDFNVGLKYGIGTLNYVGADG
                     RYGESTPLVAGDHVYDVEPKILALLEEKGALLKQAKMRHSYPHCWRTKTPLIFRATPQ
                     WFISMHQNGLLDACKKAVNKVEWVPDWGKARIDGMLEASPDWCISRQRTWGVPIALFV
                     HKDTQELHPRTAELVEAVAKKVEASGMDAWFELDAAEVLGAEAAQYEKVTDTLDVWFD
                     SGVTHFSVLDQREGLRAPADLYLEGSDQHRGWFQSSLKTSLAIRGEAPYKTVLTHGFT
                     VDAQGKKMSKSLGNTVSPQAVVKNLGADIIRLWVSATDYRGEMSVSDEILKRTADSYR
                     RIRNTARFLLSNLNGFDPAQHRVAPEQMVALDRWAVHRAAKLQKEIVACYDKYQFHLI
                     YQKLHNFCIVDLGGFYLDVIKDRQYTCKENSVARRSAQTALYHIAEALVRWVAPIMSF
                     TADELWQFLPGQRSASVHLETWYTGLFELPASEKLNSDFWQTVQDVKTAVNKQLEDAR
                     AQGVVGGSLEAEVDLYCSADLKSDLDRLGDELRFALMTSAARVHEW"
BASE COUNT          435 a          734 c          557 g          460 t
ORIGIN      
        1 ttccattcat gcacgcgcgc cgccgatgtc atcagcgcaa accgcagttc atcgcccaga
       61 cgatccagat ccgacttcag atcagcgctg caatacaggt ccacttccgc ttccaggctg
      121 ccaccgacca cgccttgcgc acgcgcgtcc tccagctgtt tgttcacggc ggttttcacg
      181 tcctgcacgg tttgccagaa atcactgttg agcttctcgc tggccggcaa ctcaaacagc
      241 cctgtgtacc aggtttccag atgcacggac gcactgcgct gccccggcag gaactgccac
      301 aattcgtcgg ccgtgaaact catgattggc gccacccagc gcaccagtgc ctcggcgatg
      361 tgataaagcg ccgtctgcgc ggaacgccgc gccacactgt tttccttgca ggtgtactgg
      421 cgatccttga tcacatccag ataaaacccg cccagatcca cgatgcaaaa gttatgcagt
      481 ttctgataga tcaaatggaa ctgatacttg tcgtaacagg ccacgatttc cttttgcaac
      541 ttcgccgcac gatgcaccgc ccagcggtcc agtgccacca tctgttccgg tgccacccga
      601 tgctgtgccg gatcaaaccc gttcaggttg gacagcagga agcgggccgt attacgaatg
      661 cggcgatagg aatcggcggt gcgcttgaga atctcgtccg acaccgacat ttcgccgcga
      721 taatccgtcg cactcaccca caggcggatg atatcggcgc ccaggttttt caccacggcc
      781 tgcggtgaca ccgtattgcc cagggatttg gacatcttct tgccctgcgc atccacggtg
      841 aagccatgcg tcagcaccgt tttatagggc gcctcacctc gaatcgccag actggttttc
      901 agcgacgact ggaaccagcc acggtgctgg tcggaacctt ccagatacag atccgccggc
      961 gcgcgcaatc cttcacgctg atccagcacg gaaaaatggg tcacgccgga atcgaaccac
     1021 acgtccagcg tgtcggtgac tttttcatac tgggcagctt cggcacccaa cacttctgcc
     1081 gcatccagtt caaaccaggc atccatcccg ctggcttcca cttttttcgc cacggcttcg
     1141 accagttcag cggtgcgcgg atgcagttcc tgcgtatcct tgtgcacgaa cagcgcgatg
     1201 ggaacacccc aggtacgctg gcgcgagata caccagtcgg gactggcctc cagcatgcca
     1261 tcgatgcgcg ccttgcccca gtccggcacc cattccacct tgttcacggc cttcttgcag
     1321 gcgtccagca agccattctg gtgcatgctg atgaaccact gcggcgtagc gcggaaaatc
     1381 agcggcgttt tcgtgcgcca gcaatgcgga tagctgtgcc gcattttcgc ttgcttcagc
     1441 aacgcgccct tttcttccag cagcgccagg attttcggct ccacgtcata cacgtgatcg
     1501 cccgccacca gcggcgtgct ttccccatag cggccatcgg cacccacata attcagcgtg
     1561 ccgatgccgt atttcaggcc tacgttaaag tcgtccatgc cgtgatcagg cgcgatgtgc
     1621 acagcgccgg taccggcatc ggtcgtcaca tgctcaccca tcaccaccgg cacaatgcga
     1681 tcgatgaagg ggtgccgcag ttgctgatgc tccagcgtgt ccccctgcgc ttcgcccaaa
     1741 actctgtaag actccacacc gtagcgcgcg caaaccgatt ccaccatgcc gctggccagc
     1801 aaaatgattt catcgccatt gccaacgttc atctgcacca ggctgtatgg caggtcgcgg
     1861 ttcagtgcca ctgcctggtt cgcgggcagc gtccagggcg tggtggtcca gatcaccact
     1921 gacaacggca acgtcacatc actcacaccg aatttctgcg cgaacgccgc cacatccacc
     1981 ggatgaaaac gcacatccac ctgggtggag gttttttcct ggtattccac ttccgcttcc
     2041 gccagcgcag agccacccac cacgctccag tacaccggtt tgaaaccttt caccaggtgc
     2101 ccgttctgcg cgatcttgcc cagtgcacga atggtgtcgg cctcggtctt gaaatccatg
     2161 gtcaggtacg gcttctgcca gtcgcc
//
LOCUS       DASUEG010000785         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1945367, whole genome shotgun sequence.
ACCESSION   DASUEG010000785 DASUEG010000000
VERSION     DASUEG010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945367"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFX11_14805"
     CDS             complement(<1..776)
                     /locus_tag="VFX11_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4939946.1"
                     /translation="MTDLSHIRNFSIIAHIDHGKSTLADRFIQICGGLSEREMEAQVL
                     DSMDLERERGITIKAHSVTLYYKSKDGATYQLNFIDTPGHVDFSYEVSRSLCACEGAL
                     LVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKIDLPQAEPERVAEEIEEIIGIEATD
                     AAQVSAKTGLGVPELLERLIQVIPPPKGERAGKLQALIIDSWFDNYLGIVSLVRVMHG
                     TLKKGDKVLIKSTGRDHIVDSVGVFSPKRTETGLLQAGEVG"
     gene            complement(904..1362)
                     /locus_tag="VFX11_14810"
     CDS             complement(904..1362)
                     /locus_tag="VFX11_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEX4939947.1"
                     /translation="MIEETGRVVAVEEGAVWVETIRQSACQSCSAKAGCGHSALSKLG
                     RQTVHFRATANSRYAVGDDVVIGVPESLVVTSSLLAYLMPLVVALGLALTADHFTGSD
                     GWAALAGMLGLAVGFAALRWHFRRNRLDDRYQPVVLRRAFAQSQCHSEYS"
     gene            complement(1432..2388)
                     /locus_tag="VFX11_14815"
     CDS             complement(1432..2388)
                     /locus_tag="VFX11_14815"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX4939948.1"
                     /translation="MVQWRTLVAGAMMLGSSLAVAGESAEQWVQKMLSAARQTSYQGH
                     SVMLSGGQITSLAIYHAPFENEVWERVVHLSGEPAEILRKGSTITCLHPDSTSRIDLQ
                     GTPMGKLTALEESAQSISRYYRFDRAANERVAGRAGVRINVEPLDAHRYGYSLWLDEG
                     TGILLKSQTNPDNGDPLEVFEFVSLEIGVPLTRDAFQPASGLQADAAAPAGSPVSAVD
                     VNTWEAEWLPEGFRAVGPAMPRPGKPGVSSRVFTDGLAAFTVFYEPQADAAVQSNRAR
                     GATVAVNRVLPGSSTLVTVVGEVPLATASKVADNVKALEPHP"
     gene            complement(2428..3099)
                     /locus_tag="VFX11_14820"
     CDS             complement(2428..3099)
                     /locus_tag="VFX11_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX4939949.1"
                     /translation="MNERNLEELSALMDGETSELEVRRVLKNIEQDQAMCQKWARYQM
                     VSAVLKGETRGQTAQWQQVDLSARISRALEDEPALSGIEPVAAPARRLADVLLKPFAN
                     VAVAASVSAAVILGWQSVNQQPVTAAGPVAMTPGVSGAVVASAPVATGLMPVAQTSGS
                     YRPATVASQPDIIRYNPDLDDRLNDYLISHSGNAAFNTASGVAPYARAVALKPAPQVP
                     QARSK"
     gene            complement(3169..3744)
                     /gene="rpoE"
                     /locus_tag="VFX11_14825"
     CDS             complement(3169..3744)
                     /gene="rpoE"
                     /locus_tag="VFX11_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016960308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX4939950.1"
                     /translation="MAERHPDQLLVERVQNGDKRAFDLLVKKYQHRVIAVVSRFVRDP
                     DEAMDVTQDAFLKAYRALANFRGESAFYTWIYRIAVNTAKNYLVSRGRRPPDSDIDVG
                     EAEQFASSEALHEFASPEQEMMKDQLEKVVFEALRELPDDLRTAVTLREFDGLSYEEI
                     AEVMDCPVGTVRSRIFRGREAIDKKIEMMNV"
     gene            3973..>4637
                     /locus_tag="VFX11_14830"
     CDS             3973..>4637
                     /locus_tag="VFX11_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4939951.1"
                     /translation="MTRFQHDILIIGSGAAGLTAALHLAPFARIGVLSKGPLTAANTY
                     YAQGGVAAVLDEGDSVAAHIEDTLIAGAGLCHEDAVRMTVENGPDAIRWLIQQGVGFT
                     TEEQDNGQMEYHLTREGGHSHRRVIHAADATGFAISSTLIEQVRKQPGVALFESRVAV
                     DVITTQKLGLPGNRALGAYVLNNETGHVEVHEARFVLLATGGVDKVYRYTSNPDTATG
                     DGIA"
BASE COUNT          933 a         1479 c         1336 g          889 t
ORIGIN      
        1 cccacttcgc ccgcctgcag caggccggtt tcagtgcgct tggggctgaa cacgcccacg
       61 ctgtcgacga tatggtcgcg accggtggac ttgatgagaa ccttgtcgcc ctttttcagg
      121 gtgccgtgca tcacccgcac cagcgacacg atgcccaggt aattgtcgaa ccaggaatcg
      181 atgatcagcg cctgcagctt gccggctcgc tcgcccttgg gcggcggaat cacctggatc
      241 aggcgttcca gcagttccgg cacgccgagg ccggtcttgg cgctcacctg cgctgcatcg
      301 gtggcctcga taccgatgat ttcctcgatt tcctctgcca cccgctccgg ctccgcctgg
      361 ggcaggtcga tcttgttcag cacgggcatc acttccagcc cctgctcgat ggcggtgtag
      421 cagttggcca cggactgggc ctcgacgccc tgccccgcat ccaccaccag cagcgcgcct
      481 tcgcaggcac acagggaccg cgacacttcg taggagaagt ccacgtgtcc cggcgtgtcg
      541 atgaaattga gctggtaggt agcgccgtct ttcgatttgt aatagagggt gacgctgtga
      601 gccttgatgg taatgccgcg ttcgcgctcc agatccatgg aatccagtac ctgggcctcc
      661 atctcgcgct cgctcagacc accgcagatc tggatgaagc ggtccgccag tgtggatttt
      721 ccgtggtcaa tgtgagcaat gatggaaaag ttgcgaatgt ggcttaagtc ggtcacaggt
      781 tcggtttact tcaaggtaat agggatggtg gcggaaaaaa ctgcggcaat tctaccggtt
      841 ttccacccgc cttgcattag atgaatcgga agcaccaggc ccagggacag gatattgaaa
      901 agactaggaa tactcgctgt gacactggct ttgggcaaag gctcttctga gcactaccgg
      961 ctggtagcgg tcatccagcc ggttacgccg gaaatgccag cgcagggcgg cgaatccaac
     1021 agccagcccc agcattcccg ccagcgctgc ccagccatcg gagccggtga aatgatctgc
     1081 cgtcagcgcc agacccaggg ccaccaccag gggcatcagg taagccagca gggaactggt
     1141 caccaccagg gattcaggca cgccgattac cacgtcgtca cccaccgcat agcgactgtt
     1201 cgccgttgca cgaaaatgca cggtctgacg gcccagtttg gacagggcac tgtgaccgca
     1261 accggctttg gcagaacagg actggcaggc ggactggcgg atggtttcaa cccagacagc
     1321 accttcttcc accgccacca cgcgcccggt ttcttcaatc atggcaggtt tcccgctgga
     1381 ttagttgaaa gcgtgtactt gaaacggcta tttgaaacca ctctactgaa atcagggatg
     1441 gggttccagc gctttcacgt tatcggcaac cttgctggcc gtggccagtg gcacctcacc
     1501 caccacggtc accagggtgg acgagcccgg caaaacgcga ttgaccgcaa cggtggcccc
     1561 acgggcgcgg ttggactgca cggcggcatc ggcctgtggc tcataaaaaa cggtgaaggc
     1621 tgccagaccg tcggtaaaaa ccctgctgct cacacccggc ttgccggggc gaggcattgc
     1681 cggccctacc gcgcgaaagc cttccggcag ccattcggcc tcccaggtat tcacgtccac
     1741 ggccgatacc ggcgatcctg cgggcgctgc agcatccgcc tgcagaccgg atgctggctg
     1801 gaacgcatca cgggtcagcg gaacaccgat ctcaagggaa acgaattcaa acacctccag
     1861 gggatcaccg ttgtcggggt tggtctggga cttcagcagg attccggttc cctcatccag
     1921 ccacagactg tagccatagc gatgggcatc caggggctcg acattgatac gcacgccggc
     1981 gcggcccgcc acacgctcat tggcggcccg gtcgaaacgg tagtagcggg agatggactg
     2041 ggcactttcc tccagggcgg taagtttgcc catcggggta ccttgcagat cgatacggga
     2101 cgtggaatcc ggatggaggc aggtgatggt ggagcccttg cgcaggattt ccgccggttc
     2161 accgctcagg tgcaccaccc gttcccagac ttcattttca aacggagcat ggtagatggc
     2221 cagcgaggtg atctgcccgc cactgagcat gacggaatga ccctgatagc tggtctgccg
     2281 cgcggcagac agcatcttct gaacccactg ctctgcggac tccccggcca cggccaggga
     2341 cgaacccagc atcatcgcgc ctgccaccag cgtgcgccac tgcaccatgt cgaatctccc
     2401 tgcctgcttg tccgaacgac gattgcctta tttggaacgg gcttgcggta cctgcggtgc
     2461 cggcttcagc gctacggcgc gggcataagg cgccacacca ctggcagtat tgaaggcagc
     2521 attgccggag tgggagatga gatagtcatt gaggcgatcg tcgagatcag gattgtagcg
     2581 gatgatatca ggctggctgg caacagtagc gggacggtag gaaccgctgg tctgcgcaac
     2641 tggcatcagg ccggttgcca cgggtgcgga cgccaccacg gcgccggaca cgcccggcgt
     2701 cattgccacc ggcccggcgg cagtcaccgg ctgctggttg acactttgcc aacccagaat
     2761 cactgccgcc gacacggagg ccgccaccgc tacgttggca aacggtttta gcagcacatc
     2821 cgccaaacgt ctggctggag ctgccaccgg ttcgatgcca ctaagggcag gttcgtcctc
     2881 cagcgcccgg gatatccgcg ccgacaggtc cacctgttgc cattgggcag tctggccccg
     2941 cgtctcgcct ttcagcacgg cgctcaccat ctggtatcgc gcccatttct ggcacatggc
     3001 ctgatcctgc tcgatgtttt tcaacacccg ccggacctcc agctccgagg tttcaccgtc
     3061 catcagggcg gacagttctt ccagatttcg ctcgttcatc tttctcacct gcctggagat
     3121 attgcgccaa caatcagtga agcctgcgct accaccgact gcaaactgtc aaacgttcat
     3181 catttcaatt ttcttgtcga tggcctcgcg gccacggaag atccgcgaac gcaccgtgcc
     3241 caccggacaa tccatgactt ccgctatttc ctcgtaactg agaccatcga actcgcgcag
     3301 tgttaccgca gtgcgcaaat cgtcgggtaa ctccctcagc gcctcgaaca cgaccttttc
     3361 cagctggtct ttcatcattt cctgttcggg gctggcgaat tcgtgcagcg cttcgctact
     3421 cgcaaactgc tcggcctcac ccacgtcgat gtcggagtcc ggcggacgcc gcccccgcga
     3481 caccagataa ttcttcgctg tattgacagc aatgcgatag atccaggtgt aaaacgcact
     3541 ctcgccgcga aaattggcta gcgcccgata tgccttgaga aaggcatcct gtgtcacatc
     3601 cattgcttcg tccgggtcac gcacaaagcg gcttacgact gcgatcaccc tgtgctggta
     3661 tttcttcacc agcaggtcaa acgctctttt gtcaccattc tgaacacgtt ccaccaacag
     3721 ctgatcggga tgtcgctcag ccattcaaca aaactcccca tccaggccct cgcaaacaac
     3781 gcgagcggca gtgttcataa agaccggaac tgcctgcaaa agttccagcc atttgtgccc
     3841 ggcacacctc gattcgagcg attatgacaa tccggctgta taataccgcc aagtcaaaac
     3901 aatacgataa ccgtttcctg cgtagccctg acccagcctt gaacagggca cccctttgtt
     3961 gtggacaacc caatgacccg attccagcac gacatcctga tcatcggcag cggtgctgcc
     4021 ggcctgactg ccgccctgca cctggcaccc ttcgcgcgca tcggggtgct gagcaagggt
     4081 ccgctgacgg ccgccaacac ctactatgcc cagggtggcg tggcggcggt gctggatgaa
     4141 ggggattcgg tagccgccca catcgaggac accctaatag ccggtgccgg cctgtgccac
     4201 gaggatgcgg tgcgcatgac ggtggaaaac ggcccggatg cgatccgctg gctgatccag
     4261 cagggcgttg ggttcactac ggaggaacag gacaacggcc agatggaata ccacctgacc
     4321 cgggaaggcg gccacagtca ccggcgcgtg atccatgccg cagacgccac gggtttcgcc
     4381 atctccagca ccctgatcga gcaggtgcgc aagcagcccg gcgtggcgct gtttgaatcg
     4441 cgggtggcgg tggatgtgat cactacccag aaactgggtc tgccgggcaa ccgcgcgctg
     4501 ggcgcctatg tactgaataa cgagaccggc cacgtggaag tacacgaggc acgcttcgtg
     4561 ctgctggcca ccggcggcgt ggacaaggtc taccgctaca ccagcaaccc ggacaccgcc
     4621 accggcgacg gcatcgc
//
LOCUS       DASUEG010000786         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_716607, whole genome shotgun sequence.
ACCESSION   DASUEG010000786 DASUEG010000000
VERSION     DASUEG010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716607"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="tagH"
                     /locus_tag="VFX11_14835"
     CDS             <1..1084
                     /gene="tagH"
                     /locus_tag="VFX11_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEX4939952.1"
                     /translation="RSTECDWVIPDQTRHLSGRHAIVSYEAGQFYITDISTNGIYLNG
                     PTPLTRNVAMPLRDEDRLLMGEIQLRVRVLMEYRQPQPPQLLPAVDGDGSEPGMASRP
                     RLKPHLQSVAAVPQLPVTGSRTGSGPRPSLKPVEEPEPVSPPAPVLPTVISPAATAQE
                     PAIENALAAFAAGAGLPVDAIRQAGAEAVLRRAGALLRQCMQGLVDNAHARASLKNEL
                     RLDMTLVDSHDNNPVKLAANADQLLQHLLVDDRGSFLPLEAGVQECFAEFQQHQWAML
                     AGMQGALQEVLLELSPVELERRFEQQRRGPTLSSKGARCWNAYRELHQDLQADENLFD
                     SLFADPFARAYDEQINLLKTSARKKG"
     gene            1329..2162
                     /locus_tag="VFX11_14840"
     CDS             1329..2162
                     /locus_tag="VFX11_14840"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX4939953.1"
                     /translation="MSKTFRPDGDGLENGMKGDRPTAFLTQPGLAGDRQAGLVWRSAA
                     ASHTGCVRRYNEDAYFANPRQGFWCVADGMGGHAAGDLAARMLVEAVAMLEAGPSLTA
                     SVELLRTAILKVNVALCGDFILAQTGGTCGSTVVALAIAGDECACLWAGDSRLYLYRA
                     GNLYQMSRDHSLVQELLDQRLIESHEAGQHPRRHVITRAVGADTELELDQIQFALLPD
                     DQLLLCSDGLYGELDADTILGVLAQPFGCEHKVNQLIERALDLGARDNVTVNLIEVYA
                     A"
     gene            2288..2980
                     /locus_tag="VFX11_14845"
     CDS             2288..2980
                     /locus_tag="VFX11_14845"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX4939954.1"
                     /translation="MPGVLSGNAFSQSDLNPIPPASGGRVEACPLRLANLGKQRGARC
                     RDGLDGGGNGPELVVVKVNGVAPFAITRSEISGNDIAAFCNQTRLCQPPMQPALPVVN
                     LPLEHIQRYAGWLTARSGSHYRLPRLDEWLAVARNDSGMADHNCRLVSGGRVLRGQRL
                     RSADEGYANSLGLLNLFGNAEEWVQQGGDFITVGGSALTDMGNCNAAYRNSRTAPADT
                     LRGFRLVRELAP"
     gene            complement(2999..4330)
                     /gene="yegQ"
                     /locus_tag="VFX11_14850"
     CDS             complement(2999..4330)
                     /gene="yegQ"
                     /locus_tag="VFX11_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEX4939955.1"
                     /translation="MKPELLSPAGTIKSMEYAFAYGADAIYAGQPRYSLRVRNNEFNR
                     EENLQKAIDMAHARGKQFYLATNLAPHNDKIRSFVRDMEPVIAMGPDALIMSDPGLIM
                     LVREHWPDQTVHLSVQANAVNWATVKFWQQQGIRRVILSRELSLDEIEEIRQRVPEME
                     LETFVHGALCIAYSGRCLLSGYMTHRDSNQGACTNSCRWKYDAHEAVETAEGDIVAAS
                     PAVQTFTPQKQQVADIENRIFLLQEENRPGEYMPAYEDEHGTYIMNSKDLRAVQHVKR
                     LAEIGVHSLKIEGRTKSHFYVGRTAQIYRQAIDDAAAGREFDWKMMDALEGLAHRGYT
                     EGFYRRHLPAEFQNYEQNTSSSGTQQFVGEIMDYDRQRGLLSVNVKNRFAVGDVLELM
                     TPQGNVTFTLGHLESLQGDTLAVAPGDGWQVRVPAPADINPEFALLMRCDR"
     gene            complement(4561..>5038)
                     /locus_tag="VFX11_14855"
     CDS             complement(4561..>5038)
                     /locus_tag="VFX11_14855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4939956.1"
                     /translation="AMSQHFVKTDIQVRFNDFDVLGHLNNATYSTYIELARVHFYRDV
                     LGMDLTQITGVVSNFNIDFKRPIKYGTPISVLSRAETECGRFVNMTFLFANANDVNKV
                     YAQATQRMVAVDPQTSQLSNLPKRVQRDMELLMSGEQAHPAVVMLGGGEASMGKVG"
BASE COUNT          922 a         1453 c         1598 g         1065 t
ORIGIN      
        1 ccgctcaacg gagtgtgatt gggtcattcc ggatcagacg cgccacctgt cgggacgtca
       61 cgccatcgtt tcctacgagg caggccagtt ttacattacc gacatcagca ccaacggtat
      121 ctatctgaac ggccctacgc cgttgacgcg caacgtggcc atgccgctgc gtgacgaaga
      181 tcgtctgctg atgggggaaa tccagcttcg ggtgcgtgtt ttgatggaat accgtcagcc
      241 gcagccacca cagctgttgc cggccgtcga tggcgatggg tcggagcccg gaatggcatc
      301 acggccgcgt ctcaagccgc atttgcagtc ggtggcggca gtgccgcaac ttcctgttac
      361 aggctcccgc actggcagcg ggccgcggcc atcgctcaaa cctgtggagg agccagagcc
      421 ggtgtcgcca cctgcgccgg tattgcccac cgtcatcagc cctgctgcaa ctgcgcagga
      481 gcccgccatc gaaaatgcac tggccgcgtt tgctgcgggt gcaggtctgc cggtggacgc
      541 cattcggcag gccggcgccg aggcagtgct gcgccgcgcc ggtgcgctgc tgcggcagtg
      601 catgcagggt ctggtggaca atgcgcacgc ccgcgccagc ctgaagaacg aactgcgcct
      661 ggacatgacg ctggttgatt cccacgacaa caatcccgtc aagttggctg ccaatgcgga
      721 tcagctgctg cagcatctgc tggtggatga ccggggttct tttttgccgc tggaagccgg
      781 ggtgcaggaa tgctttgctg aattccagca gcaccagtgg gcaatgctgg ccggcatgca
      841 gggtgcgctg caggaagtgc tgctggaact gtcgccggtg gaactggaac ggcgcttcga
      901 gcagcagcga cggggcccca cgctgagcag caagggtgcc cgttgctgga acgcctatcg
      961 tgaactgcac caggatttgc aggcagacga aaatctgttt gacagtctgt ttgccgatcc
     1021 cttcgcccgc gcctacgacg agcagatcaa tctgctgaaa acatcggcca ggaaaaaagg
     1081 ctgagcagac gaagtcttgt gatggaattt gttacgtccg gacaggtgca atgtctacgt
     1141 ctatctgtgg atgcggtctg gacgccgcga tattttccgc gccaatgcca tttccgttgt
     1201 ggtggtcctg tctctctgaa caagctgttt ttttgaatgg cgtcatgcca tcccatccag
     1261 caattcaatc ctggcattca gaggcccttg agggcctgtg acagggccga tacccgagga
     1321 acataacgat gagcaagact ttcaggccgg atggagacgg gctggaaaac gggatgaagg
     1381 gagaccgtcc gaccgcgttc ctgacgcagc ccgggctggc cggcgacagg caggcgggtc
     1441 tggtgtggcg gtccgccgcc gccagccata ccggttgtgt gcgtcgttac aatgaggatg
     1501 cctatttcgc gaatccccga cagggtttct ggtgtgtggc cgatggcatg ggggggcatg
     1561 ccgctggcga tcttgccgcg cgcatgctgg tggaggccgt ggcaatgctg gaggccggcc
     1621 ccagcctgac ggcatcggtg gagttgctgc ggacggccat cctgaaggtc aatgttgcgt
     1681 tgtgcggtga tttcattctt gcgcagaccg gtggaacctg cggcagcacg gtggtggcgc
     1741 tggccattgc cggcgatgaa tgtgcctgcc tgtgggccgg ggacagccgg ctttatctgt
     1801 accgtgccgg caatctgtac cagatgagcc gcgatcacag cctggtgcag gagctgctgg
     1861 atcagcgcct gatcgagtcc cacgaggcag gacagcatcc ccggcgccat gtcatcaccc
     1921 gggccgttgg ggcagatacc gagctggagc tggaccagat ccagttcgcc ctgttaccgg
     1981 acgaccagtt gctgctgtgc agcgacggac tgtatggcga gctggatgct gatacgattc
     2041 tgggggtgct ggcgcagcca ttcggttgcg agcacaaggt gaatcaactc attgaacggg
     2101 cactggacct gggtgcccgt gacaatgtca ctgtcaacct gatcgaagtg tatgctgcat
     2161 gaacgatgac gggcaaaagg ttgtctacac agtagtgccg aacccgacag tggcgccagc
     2221 gcttctggct cgccgtattt ttgcacgcgg tttcctgcgg caatgtgctg tcatcgccgg
     2281 actgcttgtg ccgggcgtcc tgagcggcaa tgccttttca caatctgacc tcaatccgat
     2341 cccaccggca tcgggcggtc gggtcgaggc ctgcccgttg cggctggcca acctgggcaa
     2401 gcagcgtggt gcccgctgtc gcgacggtct tgacggcggc ggcaacggac cagagttggt
     2461 tgtggtcaag gtcaacggcg tggcgccgtt tgcaatcacc cgcagtgaaa tcagcggcaa
     2521 cgacatcgct gctttctgca accagacgcg cctgtgtcag ccaccgatgc agccggcatt
     2581 gccggtggtg aatctgcccc tggaacacat tcaacggtac gccggctggc tgacggcccg
     2641 cagcggctcg cattaccgtc tgccgcgtct ggacgaatgg ctggcggttg cgcggaacga
     2701 cagtggcatg gcggatcaca actgccggct ggtgtccggc gggcgggtgt tgcggggtca
     2761 gcgcctgcgc tccgccgatg agggttatgc caacagcctg ggcctgctca atctgttcgg
     2821 caatgcggag gaatgggtgc agcagggcgg tgacttcatc actgtcggcg gctccgccct
     2881 gacggatatg ggcaactgca atgctgcgta ccggaattcc cgcaccgcgc cggctgacac
     2941 cttgcgggga ttccggctgg tacgggaact ggcgccataa aatcgcacta tggaaaaatc
     3001 agcggtcgca gcgcatcagc agcgcaaatt ccggattgat gtccgccggt gccggcaccc
     3061 gcacctgcca gccgtcgccc ggtgccactg ccagcgtgtc accctgcagc gattccagat
     3121 ggcccagcgt aaaggtgaca ttgccctgcg gtgtcatcag ttccagcacg tcacccaccg
     3181 caaagcggtt tttcacgttc actgacaaca acccgcgctg gcgatcatag tccatgattt
     3241 cacccacgaa ttgctgggtg cccgagctgg aggtgttctg ctcgtaattc tggaactccg
     3301 ccggcagatg gcgacgataa aagccttcgg tgtaaccccg gtgggccaga ccttccagcg
     3361 catccatcat tttccagtcg aactcacggc ctgctgccgc atcgtcgatg gcctgccggt
     3421 aaatctgtgc cgtgcggcca acgtaaaaat gcgatttggt gcggccctca attttcagcg
     3481 agtgcacgcc gatctccgcc agccgtttta cgtgctgcac cgcgcgcaga tcttttgaat
     3541 tcatgatgta ggtgccgtgt tcatcctcgt acgccggcat gtattccccg ggacggtttt
     3601 cttcctgcag caggaaaatc ctgttctcga tatcggccac ctgttgcttc tgtggcgtga
     3661 acgtctgcac ggcggggctg gctgccacga tgtcgccttc ggctgtttcc accgcttcgt
     3721 gggcgtcgta tttccagcgg caggaattgg tgcaggcacc ctggttggaa tcccggtgcg
     3781 tcatgtagcc cgacagcaga cagcggccgg aataggcaat gcagagcgcg ccatggacga
     3841 aggtttccag ttccatctcg ggcacgcgct gccggatttc ctcgatttca tccagcgaca
     3901 gttcccgcga caggatcacg cgcctgatgc cctgctgctg ccagaacttc acggtggccc
     3961 agttcactgc gttggcctgt accgaaagat gcacggtctg gtcgggccaa tgttcccgca
     4021 ccagcatgat caggccggga tcggacatga tcagtgcgtc cggccccatt gcgatcacgg
     4081 gttccatgtc acgtacgaag gagcggatct tgtcgttgtg gggcgccagg ttggtggcga
     4141 gataaaactg cttgccgcgg gcatgggcca tgtcgatggc cttttgcagg ttttcttccc
     4201 ggttgaattc attgttgcgc acgcgcaggc tgtagcgggg ctggccggca tagatggcgt
     4261 cggcgccgta agcgaacgcg tattccatgc tcttgatggt gcccgccggg gagagcagtt
     4321 cgggtttcat gtcacacctt cacaacatta tctgtttgca ggacagcatt catttgtttt
     4381 caggacaaat gggggcgggg attctaacgg ggtgcatgaa gcgcggcatt gaccccgggc
     4441 aagattgcgc gggcggaagg tcggttgttt gaaaaaaaac ggcagtgtgg aaggcgtgtc
     4501 cgccccgcac accgcagcat tcaggtgttg gtcagtgtca gttgccggca ggttatcggt
     4561 tcagcccact ttgcccatgc ttgcttcccc gccccccagc atcactaccg ccggatgggc
     4621 ctgttccccg ctcatcagca attccatgtc gcgctgaacg cgcttgggca ggttgctcag
     4681 ttgtgaggtc tgggggtcca ctgccaccat gcgctgggtg gcctgggcgt agaccttgtt
     4741 gacatcattg gcgttggcaa acaggaaggt catgttgacg aaacgaccgc attcggtttc
     4801 ggcgcggctg agaacgctga tgggtgtgcc gtacttgatg ggacgtttga aatcaatatt
     4861 gaaattggaa accacgccgg tgatctgggt cagatccatg cccagcacgt cgcgatagaa
     4921 atgcacgcgg gccagttcga tataagtgct gtaggtcgcg ttgttcagat ggcccagtac
     4981 gtcaaaatca ttgaagcgta cctggatgtc ggtttttaca aaatgctgac tcattgct
//
LOCUS       DASUEG010000787         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_429384, whole genome shotgun sequence.
ACCESSION   DASUEG010000787 DASUEG010000000
VERSION     DASUEG010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429384"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFX11_14860"
     CDS             complement(<1..202)
                     /locus_tag="VFX11_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MorD protein"
                     /protein_id="HEX4939957.1"
                     /translation="MTESAINHALQIEQSCALVAAAFSEGRREGVRLISGEHRGFGLG
                     PNRLRVHIPFPLLAADWTLRTLT"
     gene            791..1546
                     /locus_tag="VFX11_14865"
     CDS             791..1546
                     /locus_tag="VFX11_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939958.1"
                     /translation="MNSPAVLVTGAGTRLGSLFARHMATQGYDLALHCNASLDAARQL
                     AQELRSGGRQCEIFVQDFTEPFDTDDFIANVHARFGNLSCVINNASAYQPALSRNTSR
                     ALLESQFRVNFVTPFLLAGSFARCIGKGQVVNIIDNKIAYHQYHYSAYLLSKKSLAEF
                     TRLAALEFAPDIRVNGIAPGVTLPMESRDNAYLNWRIEGIPLRKKGSDRQLMTALDYL
                     LQNDFVTGQILFVDGGESLNQVGRNSENFLDTQ"
     gene            1600..1980
                     /gene="folK"
                     /locus_tag="VFX11_14870"
     CDS             1600..1980
                     /gene="folK"
                     /locus_tag="VFX11_14870"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007112400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4939959.1"
                     /translation="MGTNIDPELNLQKARDILKSEVSFLGEATVIKTEPDGYRDQPDF
                     HNGAYLLGTGMSYDTFNQYLKGVENRLGRVKGPIKSGPRCIDLDIIIWDGQVVHSDYT
                     QSKYYVIKPVNELLQRHAISLTQT"
     gene            2053..>2807
                     /locus_tag="VFX11_14875"
     CDS             2053..>2807
                     /locus_tag="VFX11_14875"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4939960.1"
                     /translation="MTMLDRNTIQSALLQSAADLLDESGVPAVVLDLDSLSTLSESEL
                     VRVGRWSRQQPCPVIGVGAEMSAAARCVDAVVETGSELDTLLRRIIANPQAAAVLVQV
                     LRSIEHLPPDDALVVESLGYATLQSGGEFKRWLSQHQAKNPVAPALESGPPVLCSRSG
                     DQLSLVLNRPESSNAYTVEMRDALAEVFRLVELDASIRRVTVTAKGRCFCTGGELAEF
                     GMVGSPAMGHMIRSQISPAKLLLSAADRYHFHVR"
BASE COUNT          651 a          717 c          808 g          631 t
ORIGIN      
        1 aggtcagcgt gcgcagcgtc cagtctgcag ctaaaagagg aaaggggatg tgcacccgca
       61 accggtttgg gcccagacca aaaccgcggt gctcgccgga tatcaaacgc accccttcac
      121 gacgcccttc tgaaaatgcc gctgctacca gtgcgcaact ctgctcgatt tgaagggcgt
      181 ggttgatggc tgattcagtc atggtgggcg gcgctatacc gtcattgtgg ttggatgcgg
      241 ccggcaaacg gatcgcaaat aggttttttc agaacgttcc gatggaatcc atcagccagt
      301 accagaaggc aataatcaac acgacaccac cgaaggcgac gaagagacga aggatatcta
      361 cccacatatg cgcacctcta tctatggaac actgtatatc atagattgat atgatataca
      421 gtgttccata tcttgcaacg ccaatttttc aggagtgccc ttcatgccgg cgcggtccgg
      481 gacattgatc tacagggcaa gggaggacca aactggaggc ggcaagggga aagcaagcgg
      541 aggcggtttc aataatgcat acgctcagtc aattctcggt gctgaaattg aaaccctcaa
      601 tggcaaaaac acctgatgcc agagatcgat catccagggc ttatcccgga tttttatggg
      661 gtgcttttga ttccaggcct ttggcggcga ggtgctatgt acgacggcag atttgttatg
      721 ccattcaaat agttgacaat tcgtccgtta tttgagataa ccgcaaaaat tgcgataggc
      781 ggcagcagga atgaattcac cggcagttct ggttactggg gcaggtaccc gattgggaag
      841 cctgtttgcc agacatatgg caacccaggg atatgatctg gcgctacatt gcaacgcttc
      901 tttggacgct gcgcgccagc tggcgcagga actgcgcagc ggcgggcggc aatgcgaaat
      961 attcgttcag gatttcacgg agccattcga taccgacgac tttatcgcaa atgtgcacgc
     1021 acgctttggc aatctctcgt gcgtgataaa caatgcgtcg gcctaccagc ccgcactgtc
     1081 acggaacacc agcagggcgc tgctggaatc gcaattcagg gtgaattttg tcacgccgtt
     1141 cctgttggcc ggcagttttg cacgctgtat tggcaaaggt caggtagtca atatcatcga
     1201 caacaagatt gcctatcacc agtaccacta ttctgcttat ctgttatcga agaaatcgtt
     1261 ggctgaattt acccgattgg cggcgctgga atttgccccg gacattcgtg tcaatggcat
     1321 agcgcccggg gtgacactgc ccatggaatc ccgggacaat gcatatctga actggcggat
     1381 tgaaggtata ccgctcagaa agaagggcag cgacagacag ctcatgacag cgcttgatta
     1441 tcttctgcaa aacgattttg tcacaggaca gatattgttt gtcgatggcg gtgaatccct
     1501 gaatcaggtg ggcaggaatt cggaaaattt tctggacacc cagtaacgac gtcaggtcaa
     1561 tccatcatgc aggaaccgct acatctcgct attgtgtctg tgggcaccaa catcgacccg
     1621 gagctgaatc tgcaaaaggc ccgcgatatc ctgaaaagcg aagtatcgtt tctgggtgag
     1681 gccacggtga tcaagacgga gccggatggc tatcgcgacc agcccgattt ccataatgga
     1741 gcctatctgt tgggcaccgg catgtcctat gacacattca atcaatacct gaagggtgtc
     1801 gaaaacaggc ttggtcgggt gaaagggccc ataaaatccg gtccccgctg cattgatctg
     1861 gatatcatca tctgggatgg gcaagtagtt cacagtgact atacccagag caaatactac
     1921 gtcatcaaac cagtcaacga actgctgcag cggcatgcga tcagcttgac gcaaacgtga
     1981 atgctgcggc aggtctgcac accactagcg cagggatcag gccgccgcct gtgatccggg
     2041 aatagcgacc acatgaccat gctcgaccgt aacaccattc aatcggcctt gctccagtct
     2101 gcggcagatt tgctggacga gagtggcgtt cctgctgtcg tgctggatct ggatagtctg
     2161 tctacgttga gtgagagcga gttggtgcgg gtggggcgct ggagccggca gcaaccgtgc
     2221 ccggtaatcg gggtgggtgc agaaatgtcg gcagctgccc gctgtgtgga tgccgtcgtg
     2281 gaaactggat ctgagctcga taccctgttg cgccgcataa ttgccaatcc ccaggctgcc
     2341 gcggtgctgg ttcaggtgct tcgttccatc gagcatctgc cacctgatga tgcgctcgtt
     2401 gtagagtcgc ttggctatgc caccttgcag tccggcggtg aattcaagcg ctggttgtca
     2461 caacatcagg ccaagaatcc ggtcgctccc gccctggaaa gcggcccgcc ggtgttgtgc
     2521 agtcgaagtg gagatcagct cagcctggtt ttgaaccggc ctgaaagcag caatgcctat
     2581 acggtggaaa tgcgggatgc gctggcagaa gtgtttcggc tggtggagct ggatgcgtcc
     2641 atccgccggg tcaccgtgac tgcaaaaggg cgctgtttct gcaccggtgg tgagctggca
     2701 gaatttggga tggtgggttc accggcgatg ggccacatga tccggtcgca gatctcgccg
     2761 gcgaaactgc tgctgtctgc ggcggatcgc tatcattttc atgtgcg
//
LOCUS       DASUEG010000788         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_13845, whole genome shotgun sequence.
ACCESSION   DASUEG010000788 DASUEG010000000
VERSION     DASUEG010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13845"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFX11_14880"
     CDS             <1..316
                     /locus_tag="VFX11_14880"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX4939961.1"
                     /translation="RRRPESSFKTRYGTAGQPAGAVTGCPVHHQASEAKKIPSFTISI
                     DRQLCQGHSMCMGEAPELFKVDNTGFSHILQASPDIALLEKARQAAKFCPNQAISIKV
                     DE"
     gene            365..1219
                     /locus_tag="VFX11_14885"
     CDS             365..1219
                     /locus_tag="VFX11_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939962.1"
                     /translation="MSSTQTLGGARAVPSRAGSPRWFSANPDRAWAEKFFLVYSPIWM
                     ILMGIMMPTGWVRSFSDSLLLVHGMLVAAPIFLVPLILRRHANPGRPWYDSYWFKANL
                     YIGLFGFFGNYFGSEYFFDVLGMVYNMPNVTTTLDANLVGRGEQPVPVIMYLYTHAYF
                     ITYHTTAVIVLRRIMTAGIPASSLLFLPIVFAVGYFWAWMETKAMANPMMAASFYYTN
                     MDGMLAYGSAIYALYFIASFPIYYYLDESESRRWDLFKVSAAGLSASMLTFYLLDAAA
                     WWVGSIGS"
     gene            complement(1389..2093)
                     /locus_tag="VFX11_14890"
     CDS             complement(1389..2093)
                     /locus_tag="VFX11_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939963.1"
                     /translation="MSQPTRPFLGESAETRISARRQILLETAFQMAAESGWKGLSIHQ
                     LCQEAGLNKRYFYESFADLDELGAALVDDLAERLIATGQAAVQSGLQQGFDTMALARH
                     ALRQSIGWLVEDPRRARMLFSMAGDNPRALQHRQDVIRKLAQTLSVFSIEYHQADEPH
                     VIVKVGSALLIGGTIEIVVGWLDGQLEVSLDELVEDVASFWVAVGNSAIELTKRRLEK
                     KAVGKRPKKKTKTDLD"
     gene            2256..2915
                     /locus_tag="VFX11_14895"
     CDS             2256..2915
                     /locus_tag="VFX11_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939964.1"
                     /translation="MNATAKLSTSTVPVTDQAYISQLRAMSAKQPEKAKALAWDLFKE
                     LQKTDQQYRLPGVFAQGTAPESPNGDCEGINMNLYGAPWLRGMDMLIRVGQLLGGIGW
                     AGKTFDAQNGTGYNRLTPSAKIAAVIAMPHYKMRKINGELIGFDFYHCIDNSPVAPNI
                     PVRAITYDAPEFNNPLVLPKTRDEIVEIVPNVYLGRVLLRNTFGWKLSGYFGLRYPVG
                     G"
     gene            2919..>3406
                     /locus_tag="VFX11_14900"
     CDS             2919..>3406
                     /locus_tag="VFX11_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4939965.1"
                     /translation="MTIKLKDAVVCVTGAARGIGRATAEGLAKRGARVVLGDIDEAEA
                     LKVAKTIGNGAVAIKLDVADPASFTAFVAKAKELGPISLLVNNAGIQRTGRFIDQTLE
                     SQHREIAINLGGVINGMRLVLPDMLTRDYGHIVNVASMSAKMTLPGVAVYTASKFGVA
                     SLS"
BASE COUNT          745 a          995 c          926 g          740 t
ORIGIN      
        1 tcgccgccgg cccgaatcgt cgttcaaaac ccgctatggt acggcagggc agcccgccgg
       61 cgcggtaacg ggttgcccgg tacatcacca ggcgtccgag gcgaagaaaa ttccgagctt
      121 caccatcagc atcgatcgcc aactgtgtca gggccactcc atgtgcatgg gcgaagcgcc
      181 ggaactgttc aaggtggaca acaccggctt ctcgcacatt ctgcaggcct cgccggacat
      241 cgcattgctg gaaaaagccc ggcaggccgc gaagttctgc cccaaccagg ccatcagcat
      301 caaggtggat gaataaccga tccaaccggc cgttccccat aacaacaacg aaaaggaatg
      361 cagcatgagt tcaacacaga ccctgggcgg tgcgcgcgcc gtcccctccc gcgccggttc
      421 gccgcgctgg ttttccgcca acccggatcg ggcctgggcg gaaaaattct tcctggtcta
      481 cagccccatc tggatgatcc tgatgggaat catgatgccg accggctggg tccggtcgtt
      541 cagcgattct ctgctactgg tgcatggcat gctggtggcg gcgccgattt ttctggtgcc
      601 gctgatcctg cgccgccacg ccaaccccgg ccgcccctgg tatgacagct actggttcaa
      661 ggccaatctg tatatcggcc tgtttggatt ttttggcaat tatttcggct cggaatattt
      721 cttcgatgtc cttggcatgg tgtacaacat gcccaatgtc accaccactc tggacgccaa
      781 tctggtgggg cgcggcgagc agccggtgcc ggtaatcatg tacctgtaca cgcacgcgta
      841 cttcatcacc tatcacacca ccgccgtgat cgtgctgcgc cgtatcatga cggcgggcat
      901 tcctgcgtcg agcctgctgt ttttgccgat cgtgttcgcg gtgggttatt tctgggcctg
      961 gatggaaacc aaggcgatgg ccaatccgat gatggccgct tcgttctatt acaccaacat
     1021 ggatggaatg ctggcgtatg gctcggccat ttatgcactg tatttcatcg ccagttttcc
     1081 gatttactac tatctggatg aaagcgagtc gaggcgctgg gatctgttca aggtgagcgc
     1141 cgctggattg agcgccagca tgctgacgtt ctatctgctg gacgcagcgg cctggtgggt
     1201 gggatctatt gggagttgat cagactgccg gtataacgcc gggtcatcgc agtatcgttg
     1261 gcccggcggt ttctgtcgtg gaggcaggga ccagacactc attggcaaca cagagcctcc
     1321 tatcaggtct ggagaacccc attggggact gacacgaata ccagaagctg aaacccgaat
     1381 cccggctctt aatccagatc agtctttgtt tttttcttcg gtcttttccc aacggctttc
     1441 ttttcaagtc gtctttttgt cagctcaata gcgctatttc caacagccac ccagaaactg
     1501 gcgacatctt ccaccagctc atccagtgat acctccagct gtccgtccag ccaacccacg
     1561 acaatctcga tggttccacc gatgagcaag gcagaaccca ccttgacgat tacatgtggc
     1621 tcatcagcct gatgatattc gatactgaaa acagagagcg tttgcgccag tttccggatg
     1681 acatcctgac gatgctgaag ggcgcgagga ttgtcacccg ccatgctgaa cagcatgcgg
     1741 gcccggcgag gatcttccac cagccagccg atgctctgcc tcagggcatg ccgggccaac
     1801 gccatcgtat cgaagccctg ctgcaatcct gactgcacgg cagcttgccc agtcgcaatc
     1861 agccgctcgg ccagatcatc aaccagcgcc gctcccagct catccagatc ggcaaaactt
     1921 tcgtagaaat aacgcttgtt gagccctgcc tcctgacata gctgatgaat ggacaatcct
     1981 ttccatccgg attctgcggc catctggaaa gcagtttcga gaagaatctg gcggcgggca
     2041 ctgatgcgtg tttcagcgga ctcgccaagg aacggacgtg tgggttgtga catggatgcc
     2101 gcctgcgttt ttttctgccc cctcttatag agagatcggc tgcccgaggc aagccggtat
     2161 tgactccgtg gcgtcattca ccctaacatt ggtacagtgc tgtaccagct atgctgatac
     2221 gccgccacat ccacaatagc cacggagccc ctgcaatgaa tgcgacagcc aagctcagca
     2281 cttccaccgt ccccgtaacg gaccaggcct atatcagcca actgagagcc atgtccgcaa
     2341 agcagcctga aaaggcaaaa gctctggctt gggatctttt caaggagtta cagaaaaccg
     2401 atcagcaata tcgtcttccc ggggtgttcg cccaagggac tgcaccagaa tcgccgaacg
     2461 gagactgtga agggatcaac atgaatctct atggtgcccc gtggctcaga ggcatggaca
     2521 tgctgatcag ggtgggacag ctactgggag ggatcggctg ggccggaaag actttcgacg
     2581 cccagaacgg gaccgggtac aaccgtctga cccccagtgc aaaaatcgct gcagtgattg
     2641 ccatgcctca ttacaaaatg cgaaagatca atggtgaact gatcgggttc gacttctatc
     2701 actgcatcga caactcgccg gtggcgccga acattcccgt gcgcgccatt acctatgatg
     2761 ctcccgaatt caacaatcct ctggtactgc caaaaacccg ggacgagatt gtcgagattg
     2821 ttcccaacgt ctatctgggc cgcgtgctgc tcagaaatac gtttggctgg aaactctctg
     2881 gctacttcgg cttgcgctat ccggtaggag gctgagcaat gacaatcaaa cttaaagacg
     2941 ccgtcgtctg tgtgaccggc gcagcccggg gaattggcag ggcaacggca gaaggcctgg
     3001 caaagcgcgg ggctcgcgtt gtcctcggag atattgacga agcagaagcc ttgaaggttg
     3061 caaaaacaat cggtaatggc gccgtcgcca tcaaactgga tgttgccgat ccggcatcct
     3121 ttaccgcctt tgtcgcaaag gcaaaagaac tcggccccat ctcgttgctg gtaaacaatg
     3181 ccggaatcca gcgcaccgga cgtttcatcg accagacact ggaaagccag catcgtgaaa
     3241 ttgcgatcaa tctgggtggc gtcatcaacg gcatgcggct ggtactaccg gatatgctga
     3301 ctcgcgacta cggacatatc gtcaatgtcg cctcgatgtc ggccaagatg acgctgcctg
     3361 gtgttgcggt gtacacggcc agcaaattcg gggttgccag cctttc
//
LOCUS       DASUEG010000789         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_606469, whole genome shotgun sequence.
ACCESSION   DASUEG010000789 DASUEG010000000
VERSION     DASUEG010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606469"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFX11_14905"
     CDS             <1..1398
                     /locus_tag="VFX11_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939966.1"
                     /translation="VAPASIMAATVWTTGDPTVRLRKGSAFVATLPAKPVIGLQRSEN
                     FADYCVIEGKVTVPGFQRGQAPYELTGGDVVWNAAGQPVQQYSRDAKFVVTIPKHVPM
                     PASGFPMLMFHHGAGGNAIQVYTRGPYVGTGIDPIDDVVTLPIKEVGNGPSQVAAERG
                     WASSGFGGHLGVDHLGVINGYGQFPYNVFNPVALYNNYYQMVWERVYFRRFIDQLTVN
                     SDLCPDAVAASQVAQFRFDPDMQTIMGQSLGNWTASLQLAADPDAFEGAVLTGVAGTW
                     IKLFTNSDATRASLFAGVFSLLPGESIDDAHPFLMLMEWLLGGADPVAHLDGVFRYPT
                     KTAPHVLAVSGLDDQGSGEHVQRPHLMALGVDLAGPDLGTTYDTTLFPHMAIAGAKQL
                     PYPVAGNIAVPGQDNRTGVVVRHEDTLRPDHNGHHVAFDMDAPKHQYGCLLEQLAEGK
                     TPVIGEGFEQGGACW"
     gene            complement(1406..>2138)
                     /locus_tag="VFX11_14910"
     CDS             complement(1406..>2138)
                     /locus_tag="VFX11_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX4939967.1"
                     /translation="LTTTQFAELAAQVPADFRFVIKAPGLVTDALLRDGAGRGTKVNE
                     DFLKPERARLDFVEPALAGLGTKLGVLLFQISPLPSSWWKRMPELIERLHAMLNAIRY
                     VRERAPDVIVAVEVRDGQWLTPFFTSVLRETGATYGLGLHAKMPPIKEQLPILRALWP
                     GPLVCRWNLNPLHGAFGYEEAQRQYSPYDRLVDPDPDTRAALAKVMAGTAGAGHHVYV
                     TLSNKAEGSAPLSVAALAQAVHGLR"
BASE COUNT          405 a          682 c          668 g          383 t
ORIGIN      
        1 gttgcgcccg cgtccatcat ggccgcaacg gtgtggacca ctggcgatcc caccgtgcgc
       61 ctgcgcaagg gcagcgcgtt cgtcgcgaca ctgccggcaa agcccgtcat cggattgcag
      121 cgcagcgaga actttgcgga ttactgcgtg atcgagggca aggtgaccgt gcccggattc
      181 cagcgcggac aggcgcccta tgaactgacc ggcggcgacg tggtgtggaa tgcggccggg
      241 cagccggtgc agcagtattc gcgcgacgcg aaatttgtcg tcaccattcc caagcatgtg
      301 cccatgccgg cgtccggctt tcccatgctg atgttccatc acggtgccgg tggcaacgcg
      361 atccaggttt acacccgcgg gccgtacgtt ggcaccggca ttgatccaat cgatgacgtg
      421 gtgacgctgc cgatcaagga agtgggtaac ggtccgtcgc aggtggcagc ggaacggggc
      481 tgggccagtt ccggatttgg cgggcacctg ggcgtggacc acctgggcgt gatcaatggc
      541 tacggccagt ttccctacaa tgtgttcaac ccggtggcgc tgtacaacaa ctactaccag
      601 atggtgtggg aacgggtgta tttccgccgc ttcatcgatc agctgacagt caatagcgac
      661 ctgtgcccgg acgcagtggc cgccagccag gtggcgcagt tccgcttcga tcccgacatg
      721 cagaccatca tgggccagtc cctcggcaac tggaccgcga gcctgcaact ggcggcagac
      781 cccgatgcct tcgaaggtgc cgtgctcacc ggcgtggcgg gcacctggat caaactgttc
      841 accaacagtg acgccaccag agcctcgctg tttgccggcg tcttcagcct gctgcccggc
      901 gagagtatcg acgacgccca tccgttcctg atgctgatgg aatggctgct gggcggcgct
      961 gatccggtgg cgcatctgga cggtgttttc cgctacccca ccaagacggc gccccatgtg
     1021 ctggcggtgt ccggcctgga tgaccagggc tcgggcgagc atgtgcagcg accgcatctg
     1081 atggccctgg gggtggatct ggcaggtccg gacctgggca cgacctatga caccaccctg
     1141 ttcccccaca tggccattgc cggcgcgaaa cagctgccgt acccggtggc aggcaacatc
     1201 gccgtgcccg gccaggacaa tcgcaccggc gtggtagtgc gccatgagga tacgctgcgc
     1261 ccggaccaca acggccacca tgtggcgttc gatatggacg cgccgaaaca tcaatacggc
     1321 tgtctgctgg agcaactggc ggaaggaaaa acgccggtga tcggggaagg gtttgagcaa
     1381 ggtggggctt gctggtaacg gccttttagc gcaggccgtg aactgcctgc gccaacgctg
     1441 ccaccgacaa tggcgccgag ccctctgctt tgttgctcaa agtgacatag acgtgatggc
     1501 cggcgcctgc agtgccggcc atcactttcg ccagcgcagc gcgcgtatcc gggtcagggt
     1561 ccacaaggcg atcataggga gaatactggc gttgcgcttc ttcataaccg aacgcgccat
     1621 gcaggggatt gagattccac cggcacacca gagggcctgg ccacagcgcc cgcaggatgg
     1681 gcaactgctc tttgatcggc ggcatcttgg cgtgcagacc cagaccatag gtcgcgccgg
     1741 tttcgcgtaa cacgctggtg aaaaacggtg tcagccattg gccatcccgc acctccaccg
     1801 ccacaatcac gtcgggcgcc cgctcgcgca catagcggat ggcattcagc atcgcgtgca
     1861 gtcgctcgat cagctcgggc atccgcttcc accaggatga tggcaacgga ctgatctgaa
     1921 acagcagcac gccgagtttc gtgcccaatc ccgccaacgc cggttcaaca aaatccaacc
     1981 gcgcccgctc aggcttcaga aaatcctcgt tcactttggt gccacgacca gcgccatccc
     2041 gcagcagtgc atccgtcacc agtccgggcg ccttgatcac gaaacggaaa tcagccggca
     2101 cttgcgccgc gagttcggca aactgggttg tcgtcaat
//
LOCUS       DASUEG010000790         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_186452, whole genome shotgun sequence.
ACCESSION   DASUEG010000790 DASUEG010000000
VERSION     DASUEG010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186452"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1092)
                     /locus_tag="VFX11_14915"
     CDS             complement(205..1092)
                     /locus_tag="VFX11_14915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4939968.1"
                     /translation="MFGKPRAHTPSALKLIPRVVRFDYESVMRQNRYWHGGDPVVTHF
                     FNALQAMFPEGERFFMDSARDVRDMLGKDNIPAELNEQIRLFIRQEAMHGREHDAWTN
                     ALISLGYIGMEGFDLKLKRDNQWSKKKLKPLWRLSMTAASEHFTASLAHLFIYKRPDL
                     IEKAASPFRELLVYHAMEEVEHKAVCFDLFKQAKGGYFLRSAGMIFVTIDLLVRLRNR
                     QEYLLKKDGLWNAKTQKEMRDLLWGKEGIMRVLTPYLLQYFRPDFHPWETDERRDLLA
                     KYGQEMQVIDAQQAQVSAA"
     gene            1343..3238
                     /locus_tag="VFX11_14920"
     CDS             1343..3238
                     /locus_tag="VFX11_14920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4939969.1"
                     /translation="MKNQFVVAVLLLCLSAGWCQAASLVIDDHQTAVALGPYTEFFRD
                     ETRVMDLGTVQSQGQFQPSTDINPNFGFTRDKIWLRFRLENPLDTQQIRILDVRYFLL
                     DDAILHAPDAAGGYQTILNGRRYLEQRPPESGRFYSFKLTLPAHSSQLYYMEFSSADS
                     VALPIMLSTPERQQQYQIRDTLFMTLYGGLILSTLFFALFMLASLRERVLLYYAGFLV
                     SHHLFALMLMEGVPTALFGLNSLFMTREMDSMAIDFAILIAVLFMRNFLRLEQANPAL
                     YRFTHWMVGAMLVALVQAIILPHHVSVMLTAFICMIVGACILVCCGLQARDQMEARHF
                     LMAWSAGILGATVYGLKIFTLVPVNLFTSYSWHMGTVLEAILFSYTIAQRVNTERRER
                     LRTQTELADRERALRLTQEKLLHAETAAKEELETRVRERTRDITRILAELELENRNLL
                     ELSINDGLTRVRNRRFFNDYYPQIWQDALESGRWISVVMLDIDNFKSVNDTYGHLSGD
                     RCLVAVAGVLKQMASRPGDMICRYGGEEFILVLADTDPDSARWVAERIRMKISETPIE
                     LETSTLHLTVSLGVSGMIPEPGLDPLRLVARGDEALYQSKQQGRNRVTLLGKPQANNL
                     VPLNRKA"
BASE COUNT          699 a          973 c          924 g          736 t
ORIGIN      
        1 ggttgtgccg gtatccagga cgtggtgccg gtcgccaaac tggtggcacg gatcagcacg
       61 gaatttcatg ctgcactgaa cgaacaggct gccttgctga aaaagtggtg atgccgtcgc
      121 cgcacatcca ctgtctcgcc ccacaaaaaa cgccgcacag aatgcggcgt tttttgtggg
      181 gacaacctta tctgcgaagt agtatcaggc ggcactcacc tgcgcctgct gcgcgtcgat
      241 cacctgcatt tcctggccat acttcgccag cagatcgcgg cgctcatcgg tttcccaggg
      301 atggaaatcc ggacggaaat actgcagcag gtaaggcgtc agaacccgca tgatgccctc
      361 cttgccccac aacagatcgc gcatttcctt ctgggttttg gcattccaca agccgtcctt
      421 tttcagcaga tattcctgcc ggttgcgcag gcggaccagc agatcgatcg tgacgaaaat
      481 catgccagcg ctccgcagga aataaccacc cttcgcctgc ttgaacagat cgaagcacac
      541 ggctttgtgc tccacttctt ccatcgcgtg atacaccagc agttcgcgga acggcgatgc
      601 cgccttctcg atcaggtcag ggcgcttgta aatgaacaga tgcgccagcg acgcggtgaa
      661 atgctccgac gcggccgtca tggacagtcg ccacagtggc ttcagttttt tcttcgacca
      721 ctgattgtcg cgcttgagct tcagatcaaa cccttccatt ccgatataac ccagcgaaat
      781 cagggcattg gtccaggcat catgttcgcg tccgtgcatg gcttcctggc ggatgaacaa
      841 gcgaatctgc tcgttcagtt cggctggaat gttgtctttg cccagcatgt cgcgcacatc
      901 ccgcgccgaa tccatgaaga agcgctcacc ctccggaaac atggcctgca atgcattgaa
      961 aaagtgggtc accacgggat caccaccgtg ccagtaacgg ttctgccgca tgacagactc
     1021 atagtcaaaa cggacgacgc ggggaatcag cttcagtgcg gacggggtat gggcgcgcgg
     1081 tttgccgaac ataaaaaaac ctccagttat cagactgcta ttggaggtta gtgcactgct
     1141 tatgactttg ttaggtgaat cgccgacgaa ctcttgtgga agggcgacaa ccgtatataa
     1201 cgggtagatc cagatttctg catattttag agcaactgaa tctatccaaa ccggtcatcg
     1261 acgggcaaaa ggcagggcga aaccggcaag tcggtctatt cttgaaaaat aacgcccaat
     1321 tcatccactt ctgaaaccgc acatgaaaaa tcagtttgtt gtggcggtat tgctgctctg
     1381 cctgtctgca ggctggtgcc aggccgcttc gctggtgatt gacgaccacc agaccgctgt
     1441 cgcactgggg ccttacaccg aattcttccg cgacgaaacc cgcgtcatgg acctgggcac
     1501 agtgcagtcg cagggccaat tccagccgtc caccgacatc aatccgaact ttggctttac
     1561 ccgcgacaag atctggctgc gtttccgatt ggaaaatccg ctcgatacac agcagatccg
     1621 catcctggat gtgcgctatt ttctgctgga tgatgccatc ctgcatgcgc ctgacgccgc
     1681 cggtggttac cagactatcc tgaacggacg tcgttatctt gaacagcggc cccctgagag
     1741 cgggcgtttt tacagtttca aattgacgct gcccgcgcac agcagtcagc tctattacat
     1801 ggaattcagc agcgccgatt cggtcgcgct gcccatcatg ctgtcgacac cggaacgaca
     1861 gcagcaatac cagattcgcg acaccctgtt catgacgctg tatggcggcc tgatactgtc
     1921 caccctgttt ttcgcgctgt tcatgctggc cagcctgcgg gaacgggtgc tgctgtacta
     1981 cgccggcttt ctggtttccc accatctgtt cgccctgatg ttgatggagg gtgtacccac
     2041 ggccctgttc gggctcaaca gcctgttcat gacgcgcgaa atggattcga tggcgatcga
     2101 cttcgccatc ctgatagcgg tgttgttcat gcgcaatttc ctgcgcctgg agcaggccaa
     2161 tccggctctg taccggttca cccactggat ggtgggcgcc atgctggtgg cgctggtgca
     2221 ggccatcatt ctgcctcacc atgtgtcggt aatgctcacc gcgttcatct gcatgattgt
     2281 cggagcctgt attctggttt gctgcggttt gcaggcgcgc gatcagatgg aggcacgcca
     2341 ctttttaatg gcctggtccg ccggtattct cggcgccacc gtctatgggc tgaaaatatt
     2401 taccctggtg ccggtgaacc tgttcaccag ttacagctgg catatgggca ctgtgctgga
     2461 agcgattctg ttttcctaca cgattgcaca gcgggtaaat acggaacgcc gtgaacgcct
     2521 gcgcacccag actgaactgg ccgaccgcga gcgcgccctg cgcctgaccc aggaaaagtt
     2581 gctgcatgca gaaactgccg caaaagaaga actggaaacc cgtgtgcgcg aacgtacccg
     2641 tgacatcacg cggattctgg cggagctgga actggaaaac cgcaatctgc tggagctgtc
     2701 gatcaacgac ggcctgaccc gggtgcgcaa tcgccgtttc ttcaacgact attacccgca
     2761 gatctggcag gacgcgctgg aatccggccg ctggatcagt gtggtcatgc tggatatcga
     2821 caatttcaaa tccgtcaacg acacctatgg tcatctgtcc ggcgatcgtt gcctggtggc
     2881 agtcgcaggg gtgctgaagc agatggccag ccgccccggc gacatgatct gccgttatgg
     2941 tggcgaggaa ttcattctgg tgctggcaga taccgatccg gattcggcgc gctgggtggc
     3001 cgagcgcatc cgcatgaaga tcagcgagac gcccattgaa ctggagacca gcacgctgca
     3061 tctgacagta agcctgggtg tgtccggcat gattcccgag ccgggtctgg acccgctacg
     3121 gctggtggcg cgcggcgatg aagccctgta tcaatccaaa cagcagggtc gcaaccgggt
     3181 caccctgctg ggaaagcctc aggcaaataa tctggtgccg ctcaatcgca aggcataaaa
     3241 aagggcgccg gtcgtttccg gtccggcgcc ctgtccactc tttactccgg ctgaatctgt
     3301 tcccgaatcc agcgctgcag cgcggctgtc gc
//
LOCUS       DASUEG010000791         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_694444, whole genome shotgun sequence.
ACCESSION   DASUEG010000791 DASUEG010000000
VERSION     DASUEG010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694444"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="hflX"
                     /locus_tag="VFX11_14925"
     CDS             complement(<1..729)
                     /gene="hflX"
                     /locus_tag="VFX11_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4939970.1"
                     /translation="METLFDRPQGGERAVLVHVDFPQEYTREDPQEFEMLVDSAGAIN
                     CGLVRGSRPAPDPRLFIGSGKVEELARIVEERQAELVIFNHSLSPSQERNLERELKCR
                     VLDRTGLILDIFAQRARTHEGKLQVELAQLEHMSTRLVRGWTHLERQKGGIGLRGPGE
                     TQLETDRRLLRARIKSINARLEKVRKQRDQGRRARKRAEVPAVSIVGYTNAGKSTLFN
                     RLTRSDVYAADQLFATLDPTVRRIE"
     gene            complement(784..1056)
                     /gene="hfq"
                     /locus_tag="VFX11_14930"
     CDS             complement(784..1056)
                     /gene="hfq"
                     /locus_tag="VFX11_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX4939971.1"
                     /translation="MSKGHSLQDPFLNALRKERIPVSIFLVNGIKLQGQIESFDQYVV
                     LLRNTVNQMVYKHAISTVVPARNPRAHGGGSERGGHEGGGNGEPGL"
     gene            complement(1144..2088)
                     /gene="miaA"
                     /locus_tag="VFX11_14935"
     CDS             complement(1144..2088)
                     /gene="miaA"
                     /locus_tag="VFX11_14935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX4939972.1"
                     /translation="MGPTASGKTALAVELVQRFPMDIISVDSALIYKGMDIGTAKPDA
                     ELLAKAPHRLISFLDPAESYSAADFRTDALREMADITARGRIPLLVGGTMLYFKVLRE
                     GIADLPSADEQVRAQLLVEAAQKGWPALHAELQQIDPVAAARIHPNDPQRLQRALEVY
                     RATGRTLTDWQQQQKASGVDDSWGGDADSFPYRVINIAVCPPDRAILHKRIAARFHAM
                     LEQGFYNEVKALYERGDLHPDLPSIRAVGYRQMWDCLAGKTSYAEMEERGIIATRQLA
                     KRQITWLRSWPDLNWFDSDDPNLLENVLKILPADRILI"
BASE COUNT          427 a          629 c          660 g          432 t
ORIGIN      
        1 ttcaatgcga cgcacagtgg gatcgagggt agcgaacagc tggtcggcgg cgtacacgtc
       61 ggaccgggta agccggttga acagggtgga cttgccggcg ttggtgtaac cgacgatgga
      121 gactgcgggc acttcggcgc gtttgcgggc gcggcgtccc tgatcacgct gcttgcgcac
      181 cttttccagg cgggcattga tggatttgat gcgggcccgc agcaggcgcc ggtcggtttc
      241 cagctgggtt tcacccgggc cccgcagacc gataccgcct ttctgccgct ccaggtgggt
      301 ccagccgcgc accagccggg tggacatgtg ctccagctgc gccagctcca cctgcaattt
      361 accttcatgg gtacgggcgc gctgggcaaa gatatccagg atcaacccgg tgcggtccag
      421 cacccggcat ttcagttcgc gttcgagatt acgttcctgg gagggagaca gcgagtggtt
      481 gaagatgacc agctcggcct ggcgctcttc cacgatacgg gccagctctt ccaccttgcc
      541 gctgccgatg aacaggcgcg ggtctggcgc cggacgactg ccccgcacca ggccacagtt
      601 gatcgcgccg gcggagtcca ccagcatttc gaattcctgg ggatcttccc gcgtatattc
      661 ctggggaaaa tcgacgtgaa cgagtacagc ccgctcaccg ccctggggac ggtcgaataa
      721 ggtttccatc taacggatca cctagcggct ttgtgcgtga acaggctgtc gtaccccggg
      781 gtgttacagg ccgggttcgc cgttgccgcc accctcatgg ccaccacgct cggaaccacc
      841 accgtgggca cgggggttgc gggcgggaac cacagtggaa atggcgtgct tgtagaccat
      901 ctggttgacg gtgttgcgca gcaacacaac gtactgatcg aaagactcga tctgcccctg
      961 caatttgatg ccattgacca ggaaaatgga aacgggaatc cgttctttac gcagtgcgtt
     1021 cagaaaaggg tcttgtaaac tatgcccttt agacataaag atctccttac atgtggtgcc
     1081 caaaatcggg cgaaaagagc gaaaagcaac caattgagaa aaacaacaac aaaaagctgt
     1141 cggctaaatt aggatgcggt cggccggtaa aattttcaag acgttttcca acaaatttgg
     1201 atcgtcacta tcgaaccaat tcagatccgg ccagctacgc agccacgtaa tctggcgctt
     1261 ggccagctgc cgggtggcaa tgatgccccg ttcctccatt tcagcgtagc tggttttgcc
     1321 tgccagacaa tcccacatct ggcggtaacc cacggcgcgg atcgagggca gatcgggatg
     1381 caaatcgccc cgttcgtata gcgcctttac ttcattataa aaaccctgtt ccagcattgc
     1441 atgaaagcgt gccgcaatgc gtttgtgcaa aatggcacga tccggcgggc agaccgcgat
     1501 attgatgacc cggtagggaa aactgtcggc gtcgccgccc cagctgtcgt ctactccgct
     1561 ggccttttgc tgctgctgcc agtcggtgag ggttcggcca gtagcgcggt aaacttccag
     1621 cgcccgctgc agccgctgcg gatcattggg gtggatgcgc gcagcggcca ccgggtcgat
     1681 ctgctgcaat tctgcgtgca gggccggcca gcctttctgc gccgcttcca ccagcagctg
     1741 cgcccgcacc tgttcatcgg cggagggcag atcggcaatg ccttccctta gtactttgaa
     1801 atagagcatg gttccaccca ccagcagggg aattcttccc cgggcggtaa tgtcggccat
     1861 ttcccgcagc gcatccgtgc ggaaatcggc ggcagagtag ctttcggccg ggtcaaggaa
     1921 gctgatcagg cggtggggag ctttggccag cagttccgcg tcgggtttgg cggtgccgat
     1981 atccatacct ttatagatga gcgcggaatc gacgctgatg atgtccatgg ggaagcgctg
     2041 caccagctcc accgccaggg ccgtcttgcc agaagcggtg gggcccatca ggaagatggc
     2101 gggcggcttg gcggcgtcgg cagccacggt cgaggcgcca acgacatt
//
LOCUS       DASUEG010000792         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_385195, whole genome shotgun sequence.
ACCESSION   DASUEG010000792 DASUEG010000000
VERSION     DASUEG010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385195"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..821)
                     /locus_tag="VFX11_14940"
     CDS             complement(69..821)
                     /locus_tag="VFX11_14940"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939973.1"
                     /translation="MSYDDNKVECGVHGKSSATFICQHLANGAGLGFNAGFDPDEPDA
                     LYPDAWCDECNAVLEQEGEWNDTSEAFADIKLVCAQCYCDIREKNWVQDDESLHDLIC
                     SSFEYLKARQDEFMKGYRIGEHERWDWYQETGKLIFSHDGKPVVECDIDFVGTFSSVS
                     DSWMWAWANSSFAENIKAKSWRVRAMGDEGGFLKLASAIWSATPVDGWEMTAIMAKEW
                     GAIGAYRTPDENGFVYMIITSARWIDAQKLID"
     gene            complement(880..1875)
                     /locus_tag="VFX11_14945"
     CDS             complement(880..1875)
                     /locus_tag="VFX11_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX4939974.1"
                     /translation="MTQYTEIHLIKRPDDKPVGPHLFEVVKKDIPTAGPGQILIRQTH
                     MSLDPAMLGWMSPDTESYIPPVELGTVMRSSGLGEVIESNHRRYKAGDIVTGMTGWAE
                     YILSDGRGFSKLQDGVDAETALAVFALPGLTATQGMKILEPKAGETMVVTGAAGSVGS
                     IAGQMAKADGLRVIGVVGDAEKADWIVNTLGFDGAINYKTDDLEARLKELTPNGVDVF
                     FENTGGPIQQHVFNRMNTHGRIAVCGMIADYTTKTPAPGPNWIPLIKKRIRIQGFAMP
                     DHFADAPELIAKLAPYVMQGKIKYRTHILHGLESAMTGLNLFFTGENKGKLIVRL"
BASE COUNT          449 a          667 c          483 g          518 t
ORIGIN      
        1 acaataataa aggcgccttc gcgggccgca cgaccttgcg cttaattatg cagaccttca
       61 gatagcgttc aatctatcag cttttgcgca tcgatccatc tggcactggt gattatcata
      121 taaacaaagc cgttttcatc gggcgtccta tacgcaccga ttgctcccca ttccttcgcc
      181 atgattgccg tcatttccca accatcgacc ggcgttgcag accagatagc cgaagcaagc
      241 ttgagaaaac caccctcatc tcccatggcc cgcacccgcc aggactttgc ctttatgttt
      301 tcagcaaacg agctgttcgc ccacgcccac atccagctgt cagacacact ggaaaacgta
      361 ccaacaaaat cgatatcgca ttcaaccacc ggtttaccat catgggagaa gatgagcttg
      421 cccgtctcct gataccaatc ccagcgttca tgctcgccaa tcctgtagcc cttcatgaat
      481 tcatcctgcc ttgccttaag gtattcgaag cttgagcaaa tcagatcatg aagcgactca
      541 tcgtcctgaa cccagttctt ttcccttatg tcacaatagc attgcgcgca gaccagcttg
      601 atatccgcga aggcttccga tgtatcgttc cattccccct cctgttcaag aaccgcattg
      661 cattcatcac accaggcatc gggatacaag gcatctggct cgtccgggtc aaatccggcg
      721 ttgaacccca gcccggctcc attcgcaagg tgttggcaaa tgaaggtggc ggaactcttt
      781 ccatgtacac cgcactctac cttgttgtcg tcatagctca tcgctgcatt ccttgacgta
      841 cgcgccaccg gacattcttc agtccccgtc caccaaccct caaagcctca caatcaactt
      901 ccccttgttc tcccccgtaa aaaacagatt caaccccgtc atcgcacttt ccagtccgtg
      961 cagaatatgg gtgcggtatt tgattttccc ctgcatcaca taaggcgcca gtttcgcaat
     1021 cagttccggc gcatcggcga aatgatccgg catggcaaag ccctgaatgc ggatgcgctt
     1081 cttgatcagg ggaatccagt tcggtccggg cgctggcgtc ttcgtggtgt aatcagcaat
     1141 catgccgcag acagcaatgc gaccgtgggt attcatgcga ttgaaaacat gctgctggat
     1201 ggggccaccg gtattttcaa aaaaaacatc aacgccgttg ggcgtcagtt ctttcagcct
     1261 ggcttccaga tcgtcggttt tataattgat ggcaccgtca aagcccagcg tattgacgat
     1321 ccagtcggct ttttctgcgt cgcccaccac gccaatcaca cgcaggccgt ccgcttttgc
     1381 catttgcccc gcgatcgatc ctactgaacc ggccgcaccc gtcaccacca tcgtttcgcc
     1441 ggcctttggc tccagtattt tcatgccttg cgtggcggtc agtcccggca gcgcaaaaac
     1501 agccagcgcg gtttccgcat ccacaccgtc ctgcaatttg ctgaatccac ggccgtcact
     1561 caggatgtat tcggcccaac ccgtcatgcc ggtcacaata tcacccgcct tgtagcggcg
     1621 atgattggat tcaatcacct cacccaggcc actggaccgc atcaccgtgc ccaactccac
     1681 cggcggaata tagctttcag tatccgggct catccatccc agcatggccg ggtcgagcga
     1741 catgtgggtt tgcctgatca ggatctggcc cgggcctgcc gtcgggatgt cttttttcac
     1801 cacctcaaac aggtggggac caaccggttt gtcatcgggg cgtttgatca ggtggatttc
     1861 cgtatattgc gtcatgtcgg gctccttgct ttgggggtaa atatcagaat cctggtgctg
     1921 gctatgttac ttgctggaat caggcgctgt cggggttatt tgcggatcgc cacttcccat
     1981 tggtacgatg gtacttccat ggggcttttc gggcatcccc gcaggggttc accccttccc
     2041 cgccccccca attctgctag actgcgcaca ctttctcacc gctggaaact gtatacggat
     2101 caatggccac cccaacc
//
LOCUS       DASUEG010000793         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_380581, whole genome shotgun sequence.
ACCESSION   DASUEG010000793 DASUEG010000000
VERSION     DASUEG010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380581"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..871
                     /locus_tag="VFX11_14950"
     CDS             140..871
                     /locus_tag="VFX11_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939975.1"
                     /translation="MMYQCNPFSLRSVFRRLLQLFPLPGGVIILCCVLAASAQAGVVA
                     EDQLFTPLAPSSPRNNVRLSAESLDPAKPIWVAYKLIAVSASDNPTGKIVLDAAPEGV
                     PVDLKDLKVVFLAENLKQSESAGTIGKQLEAQVGQRSPQELTEILGPELYAAFLALNE
                     KRGTILQGTKVKTYGPTGTSDATLLASIDAAEGMQPMLIKVTMGQGDMPPELAAAAET
                     RLPLSTLLLGALGIGIALTWFRSRR"
     gene            complement(919..1365)
                     /locus_tag="VFX11_14955"
     CDS             complement(919..1365)
                     /locus_tag="VFX11_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4939976.1"
                     /translation="MATGTVRFHRVLRAPPERIYRAFLTPDAMAKWLPPNGFTGKVHH
                     MDARVGGTFKMSFTNFTTQQSHSFGGEYLELVPNERLRYTDIFDDPNLPGVMQTTISL
                     RAVSVGTELNVVQEGIPEMIPTEACYLGWQESLTLLAKLVEAEIRE"
     gene            complement(1466..1861)
                     /locus_tag="VFX11_14960"
     CDS             complement(1466..1861)
                     /locus_tag="VFX11_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4939977.1"
                     /translation="MSSDKYLAGMSVRREVLGDEYVDKAVNSATDFTRPLQDMVTENC
                     WGEVWARNGLDRRTRSLVTIATLAALKASTELKTHVLGALRNGCTVDEIQEVLLHSTA
                     YCGFPAGIEAFRAAKEVVEAWQAGGFVQK"
     gene            complement(2051..2479)
                     /locus_tag="VFX11_14965"
     CDS             complement(2051..2479)
                     /locus_tag="VFX11_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4939978.1"
                     /translation="MRTLKSWLSLMLLLSLSDAFALEQSSSVKVSTVLKAETSWDGKP
                     IEYPAGKAEVTGMVIEIVPGGETGWHLHPVPSFGMVLEGELEVQLKNGAVRRLKPGEA
                     LAEVVNTLHNGRNVGTVPVKLVVFYVGSVGQPLSVKETGK"
     gene            complement(2545..>3075)
                     /locus_tag="VFX11_14970"
     CDS             complement(2545..>3075)
                     /locus_tag="VFX11_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939979.1"
                     /translation="NQFEETGVAELAQLADSSVGTFYRLIGDKDLLLMAVHQRFIGRV
                     KSLADATLDPARWEGVGLEPVISTYVSVLADSHQQSEGLLRALIRRSSADPFFRERFH
                     ELNRYIGGKFVALVLLRPHEINHPKPAKAAELASQVLLGAMNHNTLAGLGSLSGAALK
                     RELTLMVCRYLGMPGK"
BASE COUNT          653 a          978 c          806 g          638 t
ORIGIN      
        1 gaagcggctc aacgggtcat cacccactga gatgatgccg gcttcactgc agcctgcgca
       61 tttccgccct gacacaagtg cgctaccctt tatccaattg ttcccgtttc gctcacccta
      121 cccaggccag gtgtcgtcca tgatgtacca atgcaaccct ttttcactgc gatccgtgtt
      181 ccgcagactg ttgcagctat tccctctgcc gggcggcgtt atcatcctct gctgtgttct
      241 ggccgcgtcc gcacaggccg gcgtggtggc ggaggatcag ctcttcaccc cactcgcccc
      301 gtcgtcaccc cgcaacaacg tccggctgtc cgccgaatcg ctcgatccgg ccaaaccgat
      361 ctgggtggcc tataagctga tcgcggtttc ggcgtcggac aaccccaccg gcaagatcgt
      421 gctggacgcc gcaccggagg gtgtaccggt cgatctcaag gacctgaaag tggttttcct
      481 cgcggagaac ctgaagcaat ctgaatccgc cggcaccatc ggcaaacagc tggaagcaca
      541 ggttggtcag cgctcgccac aggaactgac ggaaatactg ggccctgaac tgtacgccgc
      601 ctttctggcg ctgaatgaaa agcgcggcac gattctgcag ggcaccaaag tgaaaaccta
      661 cgggccaacc ggaacgtcgg atgccaccct gctcgcctcc atcgacgccg ccgaaggcat
      721 gcaacccatg ctgatcaagg tgaccatggg acaaggtgac atgccaccgg aactggccgc
      781 agcggcagaa acacggttac cgctgtccac gctgctgcta ggtgcgctgg gcattggcat
      841 cgcgctgacc tggttccgct cgcggcgcta atgggtcccg gcgcaacccg gcattatttc
      901 aaacggcagg cgcccacctt actcgcggat ttccgcctcg accagttttg ccagcaacgt
      961 caatgattcc tgccagccca gataacaggc ctcggtggga atcatctcgg gaatcccctc
     1021 ctgcaccaca ttcagttcgg tgcctacgga cacggcccgc agcgatatgg tggtctgcat
     1081 gacaccgggc agattgggat cgtcaaaaat gtcggtgtaa cgcagccgtt cgttgggcac
     1141 cagttccaga tattcaccgc cgaaggaatg gctttgctgc gtggtgaaat tggtgaagga
     1201 catcttgaac gtgccaccga ccctggcatc catgtgatgg accttgccgg tgaacccgtt
     1261 gggcggcaac catttggcca tggcgtctgg cgtcagaaaa gcacggtaga tgcgttccgg
     1321 cggagcgcgc aacacgcggt gaaaacggac ggttccggtg gccatggcga gtttctcctt
     1381 tcgctatctg aactgctgat ccttgaacag gtgacgccca cgatatcaat caacctgggc
     1441 aggaacaacc ctgcaaaatg ccagactact tttgcacaaa accacccgcc tgccacgcct
     1501 caaccacctc ttttgcagcc cggaatgcct cgataccagc ggggaaaccg caataggcgg
     1561 tggagtgcag caacacttcc tgaatctcgt ccaccgtgca gccattgcgc agcgcaccca
     1621 gtacatgggt cttcaactct gtcgatgctt tgagcgccgc aagcgtcgca atggtcacaa
     1681 ggctgcgggt tcttctatcc agaccatttc gcgcccagac ttcgccccag caattctctg
     1741 tcaccatgtc ctgcaaggga cgcgtgaagt ccgtagcgct gttgactgct ttatctacat
     1801 attcgtcgcc cagcacttca cgccgcaccg acatgccggc cagatattta tcactgctca
     1861 tgtttttaca ccttggtttg attcatttcc tgttgcaacc acatgctgac aatggctggc
     1921 cacgctgcaa tgaccgcagc cgcgacatcc atttgcgacc ggcatcgacc agggccctgt
     1981 tctacccaga caatcccgca gtaccagcgg atgtggcgtt ttgtgggtcg tgacgggacc
     2041 gcgtgccaat ctattttccg gtttccttca ccgacagtgg ctgcccaacc gagccgacat
     2101 aaaacacaac cagtttcacc ggcaccgtgc caacattgcg gccattgtgc agtgtattga
     2161 ccacctcggc cagcgcttca cccggcttca accgtctgac tgctccgttt ttcagctgca
     2221 cctcaagctc accttccagc accatgccga aagacggaac cggatgcaga tgccagccgg
     2281 tttcgccacc gggaacaatt tctatcacca tgccggtcac ttcagccttg ccggcaggat
     2341 actcgatcgg ctttccatcc cagctggttt cggccttcag aaccgtcgac actttcacag
     2401 acgacgactg ctccagtgca aaagcatcag acagtgacag cagcagcatg agcgaaagcc
     2461 atgatttcaa tgtgcgcata aaactcttcc tgaaggtgtg tattggtggg tgcgattgga
     2521 tgttaactga aatacaactg cattctactt gcccggcatg cccagataac ggcacaccat
     2581 cagggtcagt tcacgcttca gcgcggcacc gctgagactg cccaaccccg ccagcgtatt
     2641 gtggttcatg gcgcccaaga gaacctgcga ggccagctcg gcagccttgg caggcttggg
     2701 atggttgatt tcatggggac gcagcagcac cagcgcaaca aatttgccgc cgatataacg
     2761 gttcagttca tgaaaacgtt cgcgaaaaaa aggatcggcg gagctgcggc ggatcagcgc
     2821 gcgcaacagg ccttcgcttt gctggtgact gtccgccagc acactgacgt acgtcgatat
     2881 caccggttcc agtcccacgc cctcccaccg cgccgggtcc agcgtggcat cggcaaggct
     2941 tttgacgcgc cctataaagc gctgatgcac tgccatcaat aacaggtcct tgtcaccgat
     3001 cagccgataa aaggtgccca ccgacgaatc cgccagttgc gccagctcgg ctacgccggt
     3061 ttcttcaaac tgatt
//
LOCUS       DASUEG010000794         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_297005, whole genome shotgun sequence.
ACCESSION   DASUEG010000794 DASUEG010000000
VERSION     DASUEG010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297005"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VFX11_14975"
     CDS             complement(<1..1536)
                     /locus_tag="VFX11_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEX4939980.1"
                     /translation="MPRVAFIATFAVAFLIGLIIREVVSSPIGQLPDGGEYRGALVNG
                     RLQGAGDIQWPDGRRYQGEFEQGLYHGRGIYTYSDGSRYEGQFVRGNPEGQGALYFRN
                     GDQYVGQLRAGKMHGHGRFTRKTHVYEGDFADDEFTGQGSLTVDGKLQYSGGFRDWIF
                     NGEGQYFSEGGDSWKGTFVDGNLQGAGEHSDGDGNRYVGPFEFWQYHGKGIYYDRDKS
                     RYEGEFRNGRFHGAGVMYLPEAQHGVLSYRGEWRSGNLVSADVPAFVENAADKLEQAL
                     YSEAQRLDQQLQRLQPGVAGVTELFYLGVAGDGSQRVFGREIDFITQQLQQQFPLDGR
                     AVTLINDRFRIGDASMATRVSLEKAVQALAAKMNADEDVLLLYLTSHGSEDHQFALNQ
                     NGLRLPDLDAPTLAEILRKSGIRYQILMVSACYSGGFLPVLEAPDRLVMTAAAADRTS
                     FGCSDDSDMTYFGKAFFREALPAQPDWVKAFGVARAWIEQRERQEEKTPSLPQIAVGK
                     RIES"
     gene            complement(1656..4340)
                     /locus_tag="VFX11_14980"
     CDS             complement(1656..4340)
                     /locus_tag="VFX11_14980"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4939981.1"
                     /translation="MLAAPTIPAPEITSAPQPDWLPRPQLLALLDKAGHYKLTSLQAP
                     AGSGKTTLLTQWHRQNQERHPMAWLKLEPAHNDPVRFFSDLIAAIRAVHPGFDAYYLN
                     QLADDMEFSVDAISESLRQGFAALPAELFVLLDDYQFITAPAIHRTLADVLLHLPHPV
                     HFVIASRTHPPLQLSRLKLEDELLLLDIHDLRWEPGEIEALARRLNGASLDSDDVSQL
                     AQMTEGWIAGIKLILLSSARSERQPLQQHVNGNHPEVIQYLADAVLEQQTPAVRDFLL
                     QTSVLETLEADLCNRLLDIPNAQQLLEMLMANQLFIQAVDGARPGYRYHSLFRDFLLH
                     RLRVEDPQKIPLLHRRAADWYVAQQDYVRALELLRAIDDSAGMQAVMQLCAEHWIRRG
                     ELDVLVEWANQLPDEDLVMQSDIAFPFIAGLLFSRRFNQATYFLAEMGRHQQQGTLQG
                     RYADADAMQFLHCVLELFQRDTDFMHNPLYQAEATLSRHHDLRLFYRAMRAYHHLLNG
                     RYDQAAVEAQRAKDFLSQNGHDYLSSFADLILVLTERAQGRMSAAVYRAEQAFRERQH
                     APMTPGWVNSATSIAVIRYEQNRVDETEKMLRELMSRVSSACATEVISVNYCMLARIR
                     FQQGHEREALRLLDYLARVLQHGNYERFASQIALERVLHAVAADDADMLARVERDYAL
                     QEKLAAGYWSEQATHDECRERLGIALALILQHRGELDAAESILRQLCRVTQRQGCVFR
                     WAICRTNLALLLWRRQDEAGALRLLRDTMRESHLTCFGRTLFDEAPGAAHLLRRALET
                     GVIKPLPASYLEMFGSLLAAPDAAPPDEPGPAAEVLTDKESEILRLLQAGASNKEVSR
                     CAGISLATAKWHLKNIYAKLGVGNRTDAVSKARHLRLL"
BASE COUNT          883 a         1550 c         1420 g          874 t
ORIGIN      
        1 cgattcgatc cgcttgccca cggcaatctg gggcagcgac ggcgtttttt cctcctgccg
       61 ttcgcgctgc tcgatccagg ccctcgccac accaaacgcc ttcacccagt ccggctgggc
      121 gggcagtgcc tcgcgaaaaa atgccttgcc gaaatacgtc atatcggagt cgtcggaaca
      181 gccgaacgat gtgcggtcgg cggcggcggc ggtcatcacc agccggtctg gcgcttcaag
      241 cacaggcaga aaaccgccgg aatagcaggc cgacaccatc aggatctggt aacggatgcc
      301 ggatttgcgc aggatttctg ccagcgttgg cgcatccaga tccggcagac gcaacccgtt
      361 ctggttcagc gcgaactgat gatcctcact gccgtggctg gtgaggtaca gcagcaacac
      421 gtcctcgtcc gcattcattt tcgctgccag cgcctgcacc gctttttcca ggctgacacg
      481 ggtggccatg ctggcatcgc cgatgcggaa acgatcgttg atcagggtga ccgcgcgccc
      541 gtccagcgga aactgctgct gcagctgctg tgtgatgaaa tcgatctcgc ggccgaatac
      601 gcgctgggaa ccgtcgcccg ccacgcccag gtaaaacagt tctgtcacgc cggccacacc
      661 gggctgcagc cgttgcagtt gctggtccag ccgctgcgct tcggaataca acgcctgctc
      721 cagtttgtcc gccgcatttt ccacaaatgc cggcacatcg gccgatacca ggttaccgct
      781 gcgccactcg ccacggtaac tgagcacgcc gtgctgcgcc tccggcaggt acatcacacc
      841 ggcaccgtga aaacggccgt tgcggaattc gccttcgtag cggctcttgt cgcggtcgta
      901 ataaatgccc ttgccgtggt actgccagaa ttcgaacggg cccacatagc ggttgccgtc
      961 gccatcgctg tgctcgcccg cgccctgcag attgccgtcg acgaaagtgc ccttccagct
     1021 gtcaccgccc tcgctgaaat actggccttc accgttgaag atccagtcgc ggaagccgcc
     1081 gctgtactgc agttttccat ccaccgtcag gctgccctga cccgtgaatt cgtcgtcggc
     1141 aaaatcgccc tcgtacacat gtgtcttgcg cgtgaagcgg ccatgaccgt gcatcttgcc
     1201 agcgcgcagc tggcccacgt actgatcgcc attgcggaaa tagagcgccc cctgcccctc
     1261 cggattgccg cgtacgaact ggccttcgta acggctgccg tcggagtaag tgtaaatgcc
     1321 gcgaccgtga tacaggccct gctcgaattc gccctgatat cgacgaccgt ccggccactg
     1381 gatgtcgccg gcaccctgca ggcggccgtt taccagcgcg ccacggtatt cccccccgtc
     1441 cggcaactgg ccaatgggtg acgacaccac ttcacgaatg atgagaccga taagaaacgc
     1501 cactgcaaat gtggcaatga aagcgacccg gggcatgagg aggtcctgac tggctgcgtt
     1561 ttttcaactc caatcgtgca gcttagccaa taccgccggg ttagggaacg gagcaggtgc
     1621 ctaaacggct ttttcagccg gagtaacggc gcccctcaca acaggcgcag gtggcgcgcc
     1681 ttgctcaccg cgtcggtgcg attgcccacg cccagcttgg cgtagatgtt tttcaggtgc
     1741 cacttggcgg tggccagtga aatgccggcg cagcgactca cttccttatt gctggcgccg
     1801 gcctgcagca ggcgcaggat ttcggattcc ttgtcggtca gcacttccgc cgccggaccc
     1861 ggttcatccg gcggcgcagc gtcgggggcc gccagcagcg atccgaacat ctccagatac
     1921 gacgccggca gcggcttgat cacgccggtt tccagcgccc gccgcaacag gtgcgcggcc
     1981 cccggcgcct cgtcaaacag ggtgcggccg aaacaggtga gatgggattc ccgcatcgtg
     2041 tcccgcaaca agcgcagcgc gccggcctcg tcctggcgcc gccacaacag caacgccaga
     2101 ttggtgcggc agatcgccca gcggaacaca caaccttgcc gctgggtgac gcgacacaac
     2161 tgccgcagaa tgctctccgc tgcgtcaagt tccccccggt gctgcaggat caacgccagc
     2221 gcaatgccca gccgctcgcg gcattcgtcg tgggtggcct gttcgctcca gtaaccggcg
     2281 gcaagctttt cctgcaaggc atagtcccgc tccacccgcg ccagcatgtc ggcgtcgtca
     2341 gcggccaccg catgcagcac gcgttccagc gcgatctggc tggcaaagcg ttcgtaattg
     2401 ccgtgctgca gcacccgcgc cagatagtcc agcagccgca gtgcttcgcg ctcatgcccc
     2461 tgctgaaaac ggatgcgcgc cagcatgcag tagttcaccg agatcacttc cgtggcgcag
     2521 gcgctggaca cgcgtgacat cagttcccgc aacatttttt cggtttcatc cacgcggttc
     2581 tgctcatagc ggatcaccgc gatcgacgtg gcggaattca cccagcccgg cgtcatcggc
     2641 gcgtgctgac gttcgcgaaa tgcctgctcg gcccggtaca ccgccgccga catgcggccc
     2701 tgggcgcgct cggtcagcac caggatcaga tcggcgaaac tgctcaggta gtcgtggccg
     2761 ttctggctca gaaaatcctt ggcccgttgc gcttccaccg ctgcctgatc gtagcggccg
     2821 ttaagcaggt ggtgataggc acgcatggcg cggtaaaaca ggcgcaggtc gtgatggcgc
     2881 gacagcgtgg cctcggcctg gtacagcggg ttgtgcatga aatccgtgtc gcgctggaac
     2941 agctccagca cacaatgcag gaactgcatc gcgtcggcat cggcgtaacg gccctgcaac
     3001 gtgccctgct gctggtgccg gcccatttcc gccagaaaat aggtggcctg attgaagcgg
     3061 cgggaaaaca gcaggccggc gataaacgga aacgcgatat cgctttgcat caccagatct
     3121 tcgtccggca attgattggc ccattccacc agcacgtcca gctcgccgcg gcgaatccag
     3181 tgctcggcgc acaactgcat caccgcctgc atgccggcgc tgtcgtcgat ggcgcgcaac
     3241 agttccagcg cgcgcacgta atcctgctgt gccacatacc agtcggcggc gcggcggtgc
     3301 agcaacggaa ttttctgcgg atcttccacc cgcagccggt gcagcaaaaa atcgcggaac
     3361 agggaatggt agcggtagcc cggccgtgca ccgtccaccg cctggatgaa cagctggttg
     3421 gccatcagca tttccagcag ctgctgcgcg ttgggaatat ccagcagacg attgcacagg
     3481 tccgcctcca gcgtttccag caccgacgtc tgcagcaaaa agtcccgcac tgccggcgtc
     3541 tgctgttcca gcaccgcgtc cgccagatac tggatcactt ccggatgatt gccattcaca
     3601 tgctgctgca gcggctgtcg ctcgcttcgc gccgacgaca gcaggatcag cttgatgccg
     3661 gcgatccagc cttccgtcat ctgcgccagc tggctgacgt cgtcgctgtc caggctggcg
     3721 ccgttcagcc gacgcgccag cgcctcaatc tcgcccggtt cccagcgcag atcgtggatg
     3781 tccagcaaca gcaattcgtc ttccagcttc agccggctca actgcagtgg cggatgggtg
     3841 cgcgacgcaa tcacgaaatg caccggatgt ggcaggtgca gcaatacgtc cgccagggtg
     3901 cggtgaatgg cgggcgcggt aatgaactgg taatcgtcca gcagcacgaa cagttccgcc
     3961 ggcagcgcgg cgaaaccctg gcgcagggat tcggaaatgg cgtccacgga aaattccatg
     4021 tcgtccgcca gctggttcag gtaataggcg tcgaagccgg gatgcacggc gcggatggcg
     4081 gcgatcaggt cggagaagaa gcgcaccgga tcattgtggg ccggttccag cttgagccag
     4141 gccatcgggt gacgttcctg gttctggcgg tgccactggg tcagcagggt ggttttgccg
     4201 gagccggcgg gagcctgcag cgacgtcagc ttgtaatggc cagccttgtc cagcagcgcc
     4261 agtagctgag ggcgcggcag ccagtcgggt tgcggggcag aggtgatttc cggcgccggg
     4321 atggtagggg cagcaagcat acggtactga tctttgtatt gttatgagtg atcccttcgc
     4381 caccgtacca tccagcccct gtgcggttca agcgccccgc ccgcagcaag tgtgaccggt
     4441 cagccccctc cggggagggg gcaaaccata gcccattcat gcaaaatgtt gtactagcgt
     4501 acctgccggc ctcttgcagg taagcccctt ggccctcatc acaggaataa caccatgaat
     4561 aataaaaaaa taacatggct ggcgcccctg acgctggccc tggcggcctg ccagccggcg
     4621 ggggatttcc ccgatcaggg cggactctgc gatttcgacc agctgcggga aagcgaacgt
     4681 gccgcctcgg ccgccagctt cccgattccg gtcatcagca ccatgtc
//
LOCUS       DASUEG010000795         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_456782, whole genome shotgun sequence.
ACCESSION   DASUEG010000795 DASUEG010000000
VERSION     DASUEG010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456782"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFX11_14985"
     CDS             complement(<1..861)
                     /locus_tag="VFX11_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939982.1"
                     /translation="MHLQPTKVPLGRVVTRAFYCLSASLSLLALPASAYVHDDDPNGL
                     LGSTRGGNAQWDYCYGKPDATPLPVDPRTLVQPGISDGKAVHFNAYWKECHVDPVLVK
                     EAGHPETCGELRAIFHRGEALMNTGSEGVAALFSGTRYNSLSAAAGIATFNAAQYNKL
                     WTIWGGYDERPANFDQLVANRYGSGLHSERNPYPLKGEDPNLTNGGSGQLPEMFTQVR
                     NPDGSWSGRIGVTCHGCHSGEVGNKQQGNDLGFIFGGSSATDLNLFLRDMLPLGYLAS
                     GVTPLNLTQTR"
     gene            complement(1166..1390)
                     /locus_tag="VFX11_14990"
     CDS             complement(1166..1390)
                     /locus_tag="VFX11_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEX4939983.1"
                     /translation="MRIPARFAPLVFSALLSAIMVCIVSAFVLFVAQGIHSGFLAQWI
                     RSCVTTWPVAFPTVAIVAPWVRRIVSRMTA"
     gene            complement(1448..1939)
                     /locus_tag="VFX11_14995"
     CDS             complement(1448..1939)
                     /locus_tag="VFX11_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HEX4939984.1"
                     /translation="MNDLAGTLMIGIGATAVMDLWGLARKPLLGIAPPDYGLVGRWLL
                     HMTRGRFRHDAIAAATPVQGERLAGWTAHYLIGIAFAAVLIALWGEAWIQHPTIGPAL
                     IVGIGTVAAPFLLMQPGMGAGVAASRTPRPGAARLQSVITHAVFGLGLYAAGWATHFA
                     QAS"
     gene            2021..2467
                     /locus_tag="VFX11_15000"
     CDS             2021..2467
                     /locus_tag="VFX11_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4939985.1"
                     /translation="MDITEVAKRSGVPASTLRYYEEKGLIASTGRQGLRRLFAPAVLE
                     RLALIALGRAAGFSLDEIAQMFAPEGRPQIDRKLLAEKADELDRTIRKLTAMRDGLRH
                     AAACPAPSHLECPKFRRLMGLAATAAGAGHGRKKAPPVARRTKKSG"
     gene            2667..>3190
                     /locus_tag="VFX11_15005"
     CDS             2667..>3190
                     /locus_tag="VFX11_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4939986.1"
                     /translation="MSTIILRSLATAVLLSTSTLLFATTTFNEVERVVFGPAPPAQND
                     EQAQEQSVYQQSLLPQYRVTISNFFEDGINLLTRDAQRTVSDTADYYPRLEKRVHSNG
                     ICFKGTWTITEQTPYSGYFKQGATGLLIARASTALSETRRGQPRAFGFAGKIFPTLNP
                     AATTETANFFLVDVL"
BASE COUNT          648 a          913 c          961 g          668 t
ORIGIN      
        1 gcgggtctgc gtcaggttca gtggtgtaac gcccgaggcc agataaccca gcggcagcat
       61 gtcacgcagg aacaggttca ggtcggtggc gctgctgccg ccgaaaatga agcccagatc
      121 attgccctgc tgcttgttgc cgacctcgcc gctgtgacag ccgtgacagg tgacaccgat
      181 ccgcccgctc cagctgccgt cggggttgcg cacctgggtg aacatttccg gtaactggcc
      241 gctgccacca ttggtcaggt tgggatcctc gcctttcagc gggtaggggt tgcgttcgct
      301 gtgcaggccg gaaccgtagc gattcgccac cagctggtcg aaattcgccg gacgctcgtc
      361 gtagccgccc cagatggtcc agagtttgtt gtattgcgcg gcgttgaacg tggcaatgcc
      421 ggcggcggcc gacagtgagt tgtaacgggt gccggaaaac agcgctgcca cgccttcgct
      481 gccggtgttc atcagggctt cgccccggtg gaagatcgcg cgcaattcac cgcaggtttc
      541 cggatgaccc gcttccttca ccagcaccgg atcgacgtgg cattccttcc agtacgcgtt
      601 gaagtgaacg gccttgccgt cactgatacc cggctgcacc agcgtgcgcg gatcgacggg
      661 caggggtgtt gcgtcgggct tgccgtagca gtaatcccac tgggcgttgc caccccgcgt
      721 gctgccgagc aaaccgtttg gatcatcgtc atgaacgtac gcgctggcgg gcagcgccag
      781 cagactgagt gaagccgaca ggcaataaaa tgccctggtg accactctgc cgagtggcac
      841 ctttgttggt tgtaagtgca ttgtcaaatc ctcatggcgg acggggaagt ccgcctgctg
      901 ttttttgttt gattcgtttt gttcttgtta tacagatcga tctgactgct tgaatgaggc
      961 tagcacctgg tatgaagtgt cacaagacaa aatacataaa atgtctcatt ttatatcatg
     1021 atcagccatc gcctcgcctg gatacagatg cgcaagtaat gggaaattcg cgacattttg
     1081 cccgtttatc cgatgatggg acttgcccgc gggtcgcccc ccaaaggctg cgagtcgtct
     1141 gcaggcttca ggttgcgcgc tgccatcaag ccgtcatgcg gctgacaatg cgccgcaccc
     1201 acggcgccac gatggcgaca gtcggaaacg ccaccggcca ggtggttacg cagcttctga
     1261 tccattgggc gagaaatccg gaatggattc cctgtgcaac aaaaagcaca aatgctgaca
     1321 cgatgcagac catgatggcc gacagcaggg cactgaaaac gaggggggca aagcgggcag
     1381 gaatacgcat gtcattctcc tttctgaaat tggcaattgg taagtgtgct gttgtctgcg
     1441 cctgcgctca tgacgcctgc gcgaagtgtg tcgcccagcc agcggcataa aggccgagtc
     1501 cgaaaaccgc atgggtgatg acgctttgca gccgcgctgc gccaggccgt ggtgtgcgcg
     1561 atgcggcaac gcccgccccc atgccgggtt gcatcagcag gaagggcgcc gccactgtgc
     1621 caatgccgac gatcaaggct gggccgattg tcgggtgctg aatccaggcc tcgccccaga
     1681 gggcgatcag cacagcggcg aacgcaatgc cgatgagata gtgcgcggtc cagcccgcca
     1741 gacgttcgcc ctgaacgggg gttgcggccg caatggcatc gtgacgaaag cgcccgcgtg
     1801 tcatatgcag cagccagcgt cccaccaggc catagtccgg tggggcgatg cccagcagtg
     1861 gcttgcgcgc cagaccccac agatccatca ctgcggtggc gccaattcca atcatcaatg
     1921 tgcctgccag atcgttcatg ctgttcacct cgaattggtt ttcatccggt tgtggtgtta
     1981 gcgtacaact tcaagtcgac ttgaggtcaa ggggtgtggc atggatatta ccgaggtggc
     2041 aaagcggtcc ggtgttcccg catcgacgtt gcgctattac gaagaaaagg ggctgatcgc
     2101 gtccacgggc aggcaggggt tgcgccgcct gtttgcccct gcggtgctgg agcggctggc
     2161 attgattgcg ctggggcgtg cagcaggctt ttctctggac gagatagcgc agatgtttgc
     2221 gcccgaaggc cgcccgcaga tcgaccggaa gctgttggcg gaaaaagcgg atgaactcga
     2281 ccgaacgatt cgcaagctga ctgccatgcg ggatggattg cgccatgcag ccgcgtgccc
     2341 ggcgccgagt catctggagt gccccaagtt tcgccgcttg atgggattgg ccgcgacggc
     2401 cgccggagcc gggcatggca ggaagaaagc gcctccggtc gcacgtcgga caaaaaaatc
     2461 cggctgatcg tactgttttc cagccaggat catttttata agacccacag ccgttacttg
     2521 acagccagtg gctatttttc ttttaaaccc ggggtggaac tgctactaat gagttgaatt
     2581 gaacgaccag ctgttttgct tttcccgttc gatgggatgc gtgagcatct gacaacaatt
     2641 ccaaaaaaga cattgaggtc ctgatcatga gcacaatcat cctccgttcg ttggcgacag
     2701 ccgtgctgct gtccaccagc acgctgcttt tcgcaaccac gaccttcaat gaagtggagc
     2761 gcgttgtgtt cggccctgcg ccacctgcgc aaaacgatga gcaggcgcag gagcaaagcg
     2821 tctatcagca atccctgctg ccgcaatacc gggtcactat cagtaatttt tttgaagacg
     2881 gcatcaattt gctgacccgg gatgcccagc gcacggtgtc cgacacggcg gattattatc
     2941 cccgccttga aaaacgtgta cacagcaatg gcatttgctt caagggcacc tggaccatca
     3001 cggaacagac accctattcc ggttatttca aacaaggcgc gaccgggttg ctgattgcgc
     3061 gggcatcgac ggccctgtca gaaaccagac ggggtcagcc ccgcgcattc ggatttgccg
     3121 gcaagatttt tccgacgctg aatccggctg ccaccacaga gacggcgaat ttttttctcg
     3181 tggatgtgct
//
LOCUS       DASUEG010000796         5415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_484986, whole genome shotgun sequence.
ACCESSION   DASUEG010000796 DASUEG010000000
VERSION     DASUEG010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484986"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..833
                     /locus_tag="VFX11_15010"
     CDS             111..833
                     /locus_tag="VFX11_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4939987.1"
                     /translation="MEQDQQQSHDNLFVELIQKATEKELDDAVRQFNFPVPGQGAKAD
                     VLFNSIGVFTSKGIEDTTVQDLLDAANISRRTFYKYFKNKVEVLESIYQIAAELLMAR
                     FRAVKTQSASVSEFVVQCVELFFDYHANLGPLIRMMTEEALRADSPLAPHRATLLEHM
                     VKLFDDRYFEEEGVRLDPKVYYSLIWMMESASMNILTTTPCTREVVDSYKAVMCSISA
                     RAMVSDPSQWESLPRLPTRELV"
     gene            939..2108
                     /locus_tag="VFX11_15015"
     CDS             939..2108
                     /locus_tag="VFX11_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX4939988.1"
                     /translation="MSRPAEQRILIINSTGASPLAEALGEQFNVRLLSDPTQARDESA
                     QDIDAVLMDGCKGDYDCLSVCRQLKTEAGFADVPVILMLMVAGDIELMPLYDAGVDDV
                     LTATMPRSELVARIQKAVFHRIANRQLHSRLMQANALAMSAMSDTSDLGVNIQFLVHC
                     HNCNNLDELGMLLFRTLSHYRISGSLQLRSEFEIKNLEENGLAKDLEARLLLELKDSG
                     RYVDFGRRCVMNYEQVSLLIKNMPEDPKRFGAIKDNVFALLQGASARVKAIDGARMLE
                     LERDLLRGMSHRMQDVMRQVDERYQLVMKRCAATVEDMAMKIEQSILFLDLTEPQEET
                     LTRITQSGVAAISELFNDGIRIDSSFRKLLDYLNAGFSMEGGRTPEELRKLLDRL"
     gene            complement(2103..2315)
                     /locus_tag="VFX11_15020"
     CDS             complement(2103..2315)
                     /locus_tag="VFX11_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX4939989.1"
                     /translation="MLQPGRYRHYKGKDYEVIDIATHSETGEKLVIYRTLYGNYDLWA
                     RPLAMFTETVEQDGHKVPRFNYLGPL"
     gene            2491..4332
                     /locus_tag="VFX11_15025"
     CDS             2491..4332
                     /locus_tag="VFX11_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4939990.1"
                     /translation="MLLLVGWGTAVAAVDLRADQTIYPLGQSVEWLRDSARVLTADDV
                     LQSPAEDWQASRHPVPNLGYSDAAVWFRVGLRNFNASGHWYLVINYPLIRNLDLYVVR
                     NGAVAEVFKTGDRYAFHARPIQHRDFVFPVDITPGQDVALLIRVSGPYAQQMPISLKS
                     ERAILQDEMMSSLLHGLFFGFVLVMALYNFFVFLGTRESCYFFYVLFSLSIGLFQFTQ
                     QGFAYQLLWPEEIWWQNKSTGIFIHLSLLSSYLFVTDFLNMKQQLPRFYRAFSLLALL
                     SLGTLLLTPFINEFYVMQVGVMLSLPACLLGIVGGWLTWRGGRADARVFSIAWLIFLV
                     GVIALALNKLGLVPRNFFTEHGAEVGTVVELSLLAFALAGRINQERSQRLQLELQTRE
                     LERSALLAKERALELERLSNEQLERSVRERTDDLHKALAELSDMNRKLELLNTQDPVT
                     GVGNENSFLRALKQEWDRAYRAGESLALIVVELDGYRNVVADYGQVAAEECLKNVADI
                     LARLICRPADVITRYGDKVFGVLLPNTDGAGALFLARQIADKVHEKPFDFGICQIHAS
                     VSIGVAGERPSKMDRHKELLLSAESAVYVAQSGGGNQIQLAKMPVSG"
     gene            complement(4324..>5415)
                     /locus_tag="VFX11_15030"
     CDS             complement(4324..>5415)
                     /locus_tag="VFX11_15030"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX4939991.1"
                     /translation="GDGRAVLRSCIREYLGSEALHYLGIPTSRALCIFGSGEPVRRES
                     IEQGAALIRLSPSHVRFGSFEFFFYNNKQERLQELADFVIARHFPEFQQADNPYTELF
                     RQAVSATACMIALWQAVGFAHGVMNTDNMSILGITFDYGPFGFMEDFVPGYICNHSDH
                     QGRYAFHQQPGIALWNLNALAQTLTPLVPQKDLIDTLQGYEAQLLQAYHNLMFTKLGI
                     QNPQPGDEDLVTSLLGLLSANHPDYSIFLRRLSHDASNKPVDHIADQFIDRESARAWL
                     QRYRARVQQQSASDTERQQQMLRHNPKYILRNYLAQNAIEKAEQGDFSEVEKLRTLLM
                     RPFDEQPDMEKYADFPPDWGKSLAISCSS"
BASE COUNT         1061 a         1371 c         1704 g         1279 t
ORIGIN      
        1 tatttttgtc gtttatcagg gtacactttc tagcctgttt tcgggcttct cttgcaccta
       61 tgtttattag gttgcgcccg catcattgag tagtagattg ggggataacc atggaacaag
      121 atcagcagca gtcacacgac aatctgttcg ttgaactgat tcagaaggca accgagaagg
      181 aactggatga tgctgtccgg cagttcaatt ttccggttcc tggccagggc gccaaggcgg
      241 atgtcctgtt caattcgatt ggcgtgttta ccagcaaggg catcgaggat accactgttc
      301 aggatctgct ggacgccgcc aacatttccc gacgcacatt ctacaagtac ttcaaaaaca
      361 aggtggaagt actggaaagc atttatcaga ttgcagccga gctgttgatg gcgcggttca
      421 gggctgtgaa gacgcagtcg gcctcggtca gcgagtttgt ggtgcagtgc gtggagctct
      481 tttttgacta tcacgcaaac cttgggccgc tgattcgcat gatgacggaa gaggcgctgc
      541 gggcggattc tccgctggcg ccacaccggg cgaccctgct ggagcatatg gtgaagctgt
      601 tcgatgaccg ctacttcgag gaagagggtg tgcgcctgga tcccaaggtg tattactcgc
      661 tgatctggat gatggaaagc gcctcgatga atatcctgac gacgacgccc tgtacgcggg
      721 aagtggtgga cagttacaaa gccgtcatgt gttccatcag cgcccgtgca atggtgtccg
      781 atccttcaca gtgggaatca cttcccagac tgcccacgcg cgagcttgtc tgacggcttt
      841 tccctccacc ttgttctacg ctttatgaat ggaagactgg tacccgcacg gggccagtct
      901 ttgtctgatg attcattggc ataagcaagg tgacggatat gtccagaccg gctgagcaac
      961 gcatcctcat catcaattcg accggcgcta gcccgctcgc tgaagccctg ggtgagcagt
     1021 tcaatgtacg cctgttgagc gatcccacgc aggcccgcga cgaatccgcc caggacattg
     1081 atgccgtgct catggatggc tgtaagggcg actacgattg tctgtcagta tgccgccagc
     1141 tcaagacaga ggcagggttc gccgacgtac ccgtcatcct gatgttaatg gttgcgggcg
     1201 atatcgaatt gatgccgctg tatgacgccg gggtggatga cgttctgacg gcgaccatgc
     1261 cgcgatcgga gctggtggcg cgcatccaga aagctgtgtt tcaccgcatc gcgaaccgcc
     1321 agttgcacag ccgactgatg caggccaatg cgctggcgat gagcgcaatg tcggacacca
     1381 gcgatttggg ggtcaatatc cagttcctcg tgcactgcca caattgcaat aatctggacg
     1441 aactgggcat gctgctgttc cgtaccctgt cgcactatcg catcagtggc agcctccaat
     1501 tgcgtagcga attcgagatc aagaacctgg aggagaacgg cctggcgaag gatctggaag
     1561 cgcgtttgtt gctggaactg aaggattctg ggcggtatgt ggattttggt cgtcgctgcg
     1621 tgatgaatta tgagcaggtt tcattgctga taaagaacat gccggaagat cccaagcgct
     1681 ttggtgccat caaggacaat gtgtttgccc tgctgcaggg tgcaagtgcg cgggtcaagg
     1741 cgattgacgg tgcgcgtatg ctggagctgg aacgggatct gctgcgcggc atgtcgcacc
     1801 gcatgcagga cgtcatgcgt caggtggatg aacgctacca gttagtgatg aagcgttgcg
     1861 ctgcgacggt ggaagacatg gcgatgaaaa tcgagcagtc gatcctgttt ctggacctga
     1921 ctgagccaca ggaagaaaca ctgacccgca ttacccagtc gggcgttgcg gcgatcagcg
     1981 agctgtttaa cgatggcatc cgaatcgaca gcagcttccg caaacttctg gactatctga
     2041 acgcaggctt ttcgatggaa ggcgggcgta cgccggagga actgcgaaag ttgctggatc
     2101 gtttataggg ggcccaggta gttgaagcgc ggtactttgt ggccgtcctg ctccaccgtt
     2161 tcggtaaaca ttgccagcgg acgtgcccac aggtcgtagt tgccgtagag cgtacggtaa
     2221 atcaccagtt tttctccggt ttcactgtgg gttgcaatgt cgatcacttc gtaatccttg
     2281 cccttgtaat gacggtatcg tcctggttgc agcatgccaa actcctgtct gaagtgttca
     2341 gttgattgtt cagcgaaatc tggtcggctt caagtcaatc ctgttaacat ggatgcaata
     2401 ttgctgtaat caccacaata atatcaaagg tactgtttgc aattcgggga ctggcaggtg
     2461 cgctacggac agtggattcg gtgcataggg atgctgttgc tggttggctg gggaacggcg
     2521 gttgccgcgg tggatctgcg tgcagaccag actatttacc cgttaggcca aagtgtggaa
     2581 tggctgcgcg attcagcgcg cgtgctgacg gctgatgatg tgttgcagag ccccgcggaa
     2641 gactggcagg cgagccggca tccggtaccg aatctgggct acagcgatgc tgccgtgtgg
     2701 tttcgggttg gcctgcgtaa ttttaatgcc agtggccact ggtacctcgt catcaattat
     2761 cccctgatcc ggaatctgga cctgtacgtg gtgcggaacg gcgccgtggc ggaagtgttc
     2821 aagaccggtg accgctacgc atttcatgcc cggccaatac agcatcggga cttcgtgttt
     2881 cctgttgata taacgcccgg gcaggatgtc gcgctgttga tccgggtcag cggtccctat
     2941 gcgcagcaga tgccgatcag cctgaaaagc gagcgcgcga tcctgcagga cgaaatgatg
     3001 tcgtcgctgc tgcacggctt gttcttcggt ttcgtgctgg tgatggcgct ctacaatttc
     3061 ttcgtgtttc tgggtacccg tgaatcctgc tattttttct atgtgctgtt ctcactgtcc
     3121 atcggcctgt ttcagtttac ccagcagggc tttgcctacc agttgctgtg gcccgaggaa
     3181 atctggtggc agaacaagtc gaccggcatt ttcattcatc tttcgctgct gtcgagctat
     3241 ctgttcgtca ccgactttct caatatgaag cagcagttgc cgcggttcta tcgggctttt
     3301 tccctgttgg ccctgctgtc gctgggtacg ttgctgctga cgccgtttat caatgagttt
     3361 tatgtgatgc aggttggtgt gatgctgtcg ctgccggcct gcctgttggg catcgtcggt
     3421 ggctggctga cctggcgagg cggtcgcgcc gatgcccggg tgttctcgat tgcctggctg
     3481 atttttctgg tgggcgtgat tgcgctggcg ttgaacaagc tgggattggt gccgcgcaat
     3541 ttctttaccg aacatggcgc ggaggtgggc acggtggtgg aattgtcgct gctggcattt
     3601 gcgctggcgg ggcggatcaa tcaggagcgc agtcagcgac tgcagctgga gttgcagacg
     3661 cgggagctgg agcggtcagc cttgctggcc aaggagcgcg cccttgagct ggagcgtttg
     3721 agcaacgagc agcttgagcg cagcgtgcgg gagcgcaccg atgatctgca caaggcgctg
     3781 gcggagctgt cggacatgaa ccgcaagctg gaactgctca acacgcagga cccggtgacg
     3841 ggcgttggca acgagaacag ttttctgcgc gcgctgaaac aggagtggga ccgtgcctat
     3901 cgcgccgggg aaagtctggc gttgatcgtg gtggaactcg acggctatcg caacgtggtc
     3961 gcggattatg gccaggtggc ggcggaggag tgtctgaaaa acgtggcgga tattctggca
     4021 cgattgattt gccggccggc ggatgtcatc acccgttatg gcgacaaggt gtttggtgtg
     4081 ttgctgccca ataccgatgg tgccggtgcc ctgttcctgg cgcgacagat tgcggacaag
     4141 gtgcatgaga agccgttcga tttcggtatc tgccagattc acgccagtgt cagcattggc
     4201 gttgccggcg agcgtccctc caagatggat cggcacaagg aactattgct gtccgcagag
     4261 agcgctgttt acgtggcgca aagcggcggc ggcaaccaga tccagctggc gaaaatgccg
     4321 gtatcaggat gagcagctga ttgccaggga tttgccccag tccggcggaa aatccgcgta
     4381 tttttccatg tccggctgct cgtcgaacgg acgcatcaac agtgtccgca gtttttccac
     4441 ttcgctgaaa tccccttgct ctgccttttc gatggcattc tgcgccagat agttgcgcag
     4501 tatgtatttc ggattgtggc gcagcatctg ctgctggcgt tcggtgtcgc ttgcggattg
     4561 ttgctggacg cgggcacggt agcgctgcag ccaggcgcgg gcggattcgc ggtcgatgaa
     4621 ctggtcggcg atgtgatcca cgggtttgtt ggacgcgtcg tggctgagac ggcgcaggaa
     4681 aatactgtag tcgggatgat tcgcgctgag caggcccagc agtgatgtga ccagatcctc
     4741 atcgccgggc tgtggattct ggatacccag tttggtgaac atcaggttgt gataggcctg
     4801 cagcagctgt gcttcgtaac cttgcaaggt atcgatcagg tctttttgcg gcaccagcgg
     4861 cgtcagcgtc tgcgccagcg cgttgagatt ccacagggca atgccgggct gctggtgaaa
     4921 cgcgtagcgg ccctgatgat cggagtgatt gcagatgtag cccggtacga aatcttccat
     4981 gaaaccgaac gggccgtaat cgaaggtgat gccgagaatc gacatgttgt cggtattcat
     5041 gacgccgtgg gcgaatccaa cggcctgcca tagcgcgatc atgcacgcgg tggcacttac
     5101 cgcctggcga aacagttcgg tgtacggatt gtcggcctgc tgaaactcgg ggaagtggcg
     5161 cgcgatgacg aagtcggcca gttcctgcag gcgttcctgt ttgttgttgt agaaaaagaa
     5221 ttcgaagctg ccgaaacgca catggctggg tgacaggcgg atcagcgctg caccctgttc
     5281 gatggattcg cgtcgcacgg gttcgccgct gccgaaaatg catagcgcgc gggaggtggg
     5341 gatgccgaga taatgcagcg cctcgctgcc caggtattcg cggatgcagg aacgcaacac
     5401 ggcacggcca tcgcc
//
LOCUS       DASUEG010000797         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1180784, whole genome shotgun sequence.
ACCESSION   DASUEG010000797 DASUEG010000000
VERSION     DASUEG010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180784"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1510)
                     /locus_tag="VFX11_15035"
     CDS             complement(68..1510)
                     /locus_tag="VFX11_15035"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX4939992.1"
                     /translation="MSIEAQGLIKPHSSKFSLLARCLDATMVILGLSLVTPLHLGEFN
                     AGNITSAFIAALFLQLLGEFVEIYRSWRSESVWAEARQVLFCWFTSFSLLLLLQGALH
                     MEQDILLWSAYLQWFTLSLFLLLGWRFIARNLLYRLRAMGFNTRRVAVVGSSEIALET
                     ARRLLDCTWGGYRFAGFYDDRQHAEQASLDERRLRHQEAVVEQYAGNLEQLVQDARSG
                     RLDSIFLAMPMNAELRIQAITRELTNSTASVYLIPDLFTFELLHARSINLNGIPAISI
                     VGEPNRGVQSLLKRLVDLVGASLILTIIALPMLAIAFAIKFTSRGPVFFRQTRYGLDG
                     KPFKVWKFRTMSVCEDNDDDIRQAQKNDSRLTPIGGFLRRTSLDELPQFFNVLFGNMS
                     IVGPRPHAVAHNELYRSKINSYMLRHKIKPGITGWAQVNGWRGETDTLEKMQKRIDFD
                     LYYIRHWSIAMDIKIVFMTIFKGFSGANAY"
     gene            complement(1711..2439)
                     /locus_tag="VFX11_15040"
     CDS             complement(1711..2439)
                     /locus_tag="VFX11_15040"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX4939993.1"
                     /translation="MIDLHCHLLPGIDDGPRTMAESVELARLAVRNGITHAVVTPHIH
                     AGRWNNDRVGIERLTREFRQQLAQAGVPLQLGAAAEVRLDAEILPWLEQDRLPFLGTY
                     NGKRVLLLELPHSHIPLGAENLIAWLLQRNILPLIAHPERNKDVIRKPDKIRPLIKQG
                     CLLQLTSGAIAGQFGKPAQQCAHQLLELDAVFVIASDAHNNGARPPDLVCGRDAASAI
                     VGAERAQKLVTTNPMELVAGQFVN"
     gene            complement(2436..4685)
                     /locus_tag="VFX11_15045"
     CDS             complement(2436..4685)
                     /locus_tag="VFX11_15045"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX4939994.1"
                     /translation="MYASSRRTEALLDANIIDIRHYWNVLMHNKWWILALALILASLA
                     GLIASRIEPVYRATTTVLIENSETQVTSIDDLYGLNTRAKEYYLTQFEILKSRELAEQ
                     LVDRMQLTSHPEFDPRQNATFDWHDYVPVWLDRLNQWFPRDSAPLTDEQVRAQVLNQF
                     IKSLEVEPVNNTQLVRINFDSHDPALAARVADEMADVYIESNLEARLQMTQKAAGWLR
                     GRLEALSTRLKESEARLQEYREQETLVDVAGVNSINARELDELTTRYVAASNVRAQAQ
                     NLLDQVRAAGPSPSAEQLMALPVILRHDLVKSLKNAQVKADGKVAELSKRYGQRHPAM
                     VAARAEAEQARAELLQQVQRVAQGIESDYQAARESERAIEIQLKKAKQDAQSVNRKEF
                     KLNELQREVETNRQLYDMFLTRAKETGEAGGLQMAHARVVDPAQLPRVPVAPKTNLIV
                     ALALLGGLGLGVLLAFVRDALDNTVKTPEDIEEKLNTRMLGFLPRTKARARHLPVEGF
                     LSANFSRFAEAVRSLRTDIMLSSIDQPYKTVVVTSTLPGEGKSTVSLNLAEALGQMEK
                     VLLIEADLRKPVLARALKIPLDTPGLSALVSGQAELKACITSLPERGFDVILAGSLPG
                     NPQELLSSKRLHIMLQALKRHYDRIVIDTPPTQPVSDTLVLSKFADAVIYVVKAGDTP
                     LRLVAKGIRRLVDARAPVLGVVLNQVDLRRKNHYGDYYAEEYSHYDTKMAILPTQQRR
                     KSHIEPAVS"
     gene            complement(4696..>5032)
                     /locus_tag="VFX11_15050"
     CDS             complement(4696..>5032)
                     /locus_tag="VFX11_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX4939995.1"
                     /translation="VKATGRTLGQLQNEITAGLKGDYLLDPKVSVTILEYRQFYINGE
                     VKRPGGFAFKPGLTVHQAISMAGGMTERASTNKISVIAESDPNRKKPADLNTRVHPGD
                     IITIEQSFF"
BASE COUNT          929 a         1454 c         1569 g         1080 t
ORIGIN      
        1 ctgggaaagt tgggtcagat gtgacgcttg agaaagatcg ttcatgctgt tacctggtta
       61 cccgagctca ataggcattc gcgccgctga atcccttgaa aatggtcatg aacacaatct
      121 tgatgtccat ggcgatggac cagtggcgga tgtagtagag atcgaagtcg atgcgcttct
      181 gcattttttc cagggtgtcg gtttcaccgc gccagccgtt tacctgcgcc cagccggtga
      241 tgccgggttt gatcttgtgg cgcagcatgt agctgttgat cttgctccgg tacagttcgt
      301 tgtgcgcaac ggcatgcggg cgcgggccga cgatggacat gttgccgaac agcacgttga
      361 agaactgcgg cagttcgtcc agggatgtgc gacgcagaaa gccgccaatg ggggtcagcc
      421 ggctgtcatt tttctgggcc tggcggatgt cgtcgtcgtt gtcctcgcac acgctcatgg
      481 tgcggaattt ccagaccttg aacggcttgc cgtccaggcc gtaacgggtc tggcggaaaa
      541 ataccggacc gcgggaggtg aatttgatgg caaacgcgat ggccagcatg ggcagagcga
      601 tgatggtgag gatcaggctg gcgcccacca gatccaccag tcgcttgagg agtgactgca
      661 cgccacggtt gggttcgccc acaatgctga tggcgggaat cccgttcagg ttgatgctgc
      721 gggcgtgcag cagttcgaac gtgaacaggt ccggaatcag gtacacggac gcagtggaat
      781 tggtcagttc gcgggttatg gcctggatgc gcagctccgc gttcatgggc atggcgagaa
      841 aaatgctgtc gaggcggccg gagcgggcgt cctgcaccag ctgttccagg ttgccggcgt
      901 actgttccac cacagcctcc tgatgacgca gtcgacgttc gtccagcgag gcctgctcag
      961 cgtgttggcg gtcgtcgtag aaaccggcaa atcggtagcc gccccaggtg cagtccagca
     1021 ggcgacgggc tgtctccagg gcaatttcac tgctgcccac cacagccacg cgccgggtat
     1081 tgaagcccat ggctcgcagc cggtacagca gattgcgggc aatgaaacgc caacccagca
     1141 gcaggaacag gctcagcgta aaccactgca ggtaggccga ccacagcagg atgtcctgtt
     1201 ccatgtgcaa cgcaccctgc aacagcagca acagtgaaaa actggtgaac cagcagaaca
     1261 gcacctggcg tgcttcggcc cagacggatt ccgaacgcca ggaacggtag atttccacga
     1321 attcgcccag cagctgcaaa aacagggcgg ctatgaaggc actggtaatg ttgccggcat
     1381 tgaattcacc cagatgaaga ggcgtgacca gggacaaacc cagaatcacc atcgtcgcgt
     1441 ccaggcagcg ggcaagcagg gaaaatttgg aactgtgcgg tttgatcaga ccttgtgctt
     1501 caatgctcat aaaatattcc tcgcaattta accggggtta caacgtagcc aagggttaac
     1561 ttgcctttcg tttcatccgt gatcgatccg gcgttctact gaattccggg catgcagtga
     1621 tcattccagt ttgtggcatg ccgtcacgaa gacgagcact ttaaccaaat atcgcgctgg
     1681 attctgtgat ggcattgacc atcagccaat tcagttaacg aactggcccg ccaccagttc
     1741 catgggatta gtggtgacca gcttttgcgc acgttctgcg cctacgatgg cgctggctgc
     1801 gtcgcgtccg cacaccagat ccggcggccg cgcgccattg ttgtgggcat ccgatgcaat
     1861 gacgaatacc gcatccagtt cgagcagctg gtgcgcgcac tgctgtgcgg gtttgccgaa
     1921 ctgtcctgcg atcgcacccg atgtgagctg cagcaggcat ccctgcttga tcagggggcg
     1981 aatcttgtcc ggtttgcgga tcacatcctt gttgcgttca ggatgggcga tcagcggcag
     2041 aatattgcgt tgcagcagcc aggcaatcag gttctcggcg cccagcggaa tgtggctgtg
     2101 gggcagttcg agcagcagta cgcgcttgcc attgtaagtg cccagaaacg gcagccggtc
     2161 ctgttccagc catggcagta tttcagcatc cagccgcacc tcggcggccg cgcccagctg
     2221 caagggcacg cctgcctgtg ccagctgctg gcggaattcc cgggtcagcc gttcgatgcc
     2281 aaccctgtca ttgttccagc gtcccgcatg aatgtggggc gtgaccacgg cgtgggtgat
     2341 gccgttgcgc actgccagcc gcgcgagctc gaccgattcc gccatggtgc gcggaccgtc
     2401 atcgataccc ggcagcaaat ggcagtgcag atcgatcatg acacggccgg ttcaatatgg
     2461 cttttgcgcc gctgctgcgt tggcaggatc gccatcttgg tgtcgtaatg gctgtactct
     2521 tcggcgtagt aatcgccgta gtggttcttg cggcgcaggt ccacctggtt gagcacgaca
     2581 cccagtaccg gggcacgggc atccaccaga cgccggatac ccttggccac cagtcgcaac
     2641 ggtgtgtcac ccgctttcac cacgtagatg acggcatcgg cgaatttcga cagtaccagc
     2701 gtgtcgctaa caggctgggt cggcggcgta tcgatcacga tgcggtcgta atgtcgcttc
     2761 agtgcctgca acatgatgtg caaccgtttg gacgacagca gttcctgcgg attgccgggc
     2821 agtgagccgg ccaggatcac atcgaagccc cgctcgggca gggatgtgat acaggctttc
     2881 agttcggcct ggccggagac gagcgcggac aggccgggcg tatccagcgg aattttcagt
     2941 gcccgcgcca gtaccggttt gcgcaggtcc gcctcgatca gcagtacctt ttccatctgc
     3001 cccagcgctt ccgccagatt cagcgatacg gtggatttgc cctcgcccgg cagtgtggag
     3061 gtcaccacca ctgtcttgta gggctggtcg atgctggaca gcatgatgtc cgtgcgcagc
     3121 gaacgcactg cttcggcgaa gcggctgaaa ttggcggaca ggaacccttc caccggcagg
     3181 tgacgggcgc gcgctttggt acggggcagg aagcccagca tgcgcgtgtt gagtttttcc
     3241 tcgatgtctt ccggggtttt caccgtgttg tccagagcat cgcgcacaaa cgccagcaat
     3301 acccccaggc ccaggccgcc cagcagtgcc agcgccacga tcagattggt tttcggtgcc
     3361 accggcacgc gcggcaactg cgcgggatcc accacgcggg catgggccat ctgcagaccc
     3421 cctgcttcac cggtttcctt ggcgcgggtg aggaacatgt cgtacagctg gcggttggtt
     3481 tccacttcgc gttgcaattc attcagcttg aattccttgc ggttcacact ctgggcgtcc
     3541 tgcttggctt ttttcagctg gatttcgatg gcgcgttcag actcgcgggc ggcctggtaa
     3601 tccgattcga tgccctgtgc cacgcgctgc acctgctgca gcagttcggc ccgtgcctgt
     3661 tccgcttccg cccgtgcggc gaccattgcc gggtgacgct gcccgtaacg tttgctcagt
     3721 tccgccactt tgccatcggc tttcacctgg gcgttcttga gggacttcac cagatcgtgg
     3781 cgcaggatca ccggcagggc catcaactgt tcggccgagg gcgaagggcc ggcggcccgt
     3841 acctggtcca gcaggttctg cgcctgggcg cggacgttgc tggcggcgac gtagcgcgtg
     3901 gtcagttcat ccagctcacg ggcattgatg gagttcacgc cggcaacgtc caccagggtt
     3961 tcctgttcgc ggtattcctg cagccgggct tcggattcct tcagccgggt gctgagagct
     4021 tccagccggc cgcgcagcca gccggccgct ttctgcgtca tctgcagacg cgcttccagg
     4081 ttgctttcga tgtagacatc ggccatttca tcggcgacgc gcgctgccag cgccggatcg
     4141 tgggaatcga aattgatgcg caccagctgg gtattgttca ccggctcgac ttccagcgac
     4201 ttgatgaact ggttcagcac ctgggcccgc acctgctcgt cagtgagcgg ggcgctgtcg
     4261 cgcggaaacc actggttcag gcgatccagc cagacgggaa cgtagtcatg ccagtcgaag
     4321 gtggcgttct ggcgtggatc gaattccggg tggctggtca gctgcatccg gtccaccagc
     4381 tgctcggcca gttcgcggga tttcagaatc tcgaactgcg tcagatagta ttccttggca
     4441 cgggtgttga ggccgtacag gtcgtcgatc gaggtcacct gggtttcgct gttttcgatc
     4501 agcacggtgg tggtggcgcg gtagaccggc tcgatgcggc tggcgatcag tccggccagt
     4561 gatgccagga tcagcgccag ggcgagaatc caccatttgt tgtgcatgag cacattccag
     4621 taatggcgga tgtcgatgat gttggcatcg agcagggcct cggtgcgacg tgagcttgcg
     4681 tacatgccga aaacctcaga aaaaactttg ttcaatggta atgatgtccc cgggatgaac
     4741 ccgggtgttg agatccgccg gctttttccg gttcggatcg gattccgcga tgaccgaaat
     4801 cttgttggtg gaggcgcgtt ccgtcatgcc tcccgccatc gagatcgcct ggtgtacggt
     4861 caggccaggc ttgaaggcga aaccgccggg tcttttcact tcgccattga tgtagaactg
     4921 gcggtattcc aggatggtga cgctgacttt cgggtccagc aggtaatccc ctttcaggcc
     4981 ggcggtgatc tcattctgca actgtccgag ggtgcgtccg gtcgccttga cc
//
LOCUS       DASUEG010000798         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_634223, whole genome shotgun sequence.
ACCESSION   DASUEG010000798 DASUEG010000000
VERSION     DASUEG010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634223"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2232
                     /locus_tag="VFX11_15055"
     CDS             <1..>2232
                     /locus_tag="VFX11_15055"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX4939996.1"
                     /translation="NLPQATEDVGYVATIVATDPNAGDLIAYSLVAGPTGLVIDAASG
                     QINWLPQDNQTGSHTLLVRATDSAGLFSEASFLIQVLPVNDAPVITSSPSASAQSQSA
                     YRYQVAVEDPDAGDTLAFALTQAPAGMTISSTGLIEWLPPSNAAASYAVQVTVTDAIG
                     ASDSQSFSISLLNREPVITSLPAAGVPERQLYSYPVQAQDADGDTLSFALQNAPASMF
                     INAASGLLQWQTQAGDEGSYAVVVTVADGRGGFATQNVLVTIDPPANTAPVISSTPVL
                     AATEGAAYAYQVLAQDGEADPLSFALTSAPQGMVMSAGGLISWTPDATQVGEHAVAVQ
                     VSDGRAQASQSFTLVVNAKPNTAPQISSVPVMAVQANTAYQYQVVATDVDNEALTFSL
                     PTAPAGMSISSSGLVNWTPDDTQLGGHAVVVRVTDARGAFVQQSFTLAVQAVVNHPPV
                     ISSAPVTVALVGQSYSYTVAASDPENAPLTYSLVQAPAGMSMTMFGDVSWTPLATQVG
                     SHAVVVQVTDGTTPVTQNFNVVVSEPADLDVSIQLSDDVVAPGEAVTIQVVAADAINP
                     VYALTVNGSALTVDGNGVAQFSSTTSGIYQLIAQVQSADGSSGTGQTVLRVSVAGDTV
                     APVANIIAPADGTTVYDATAITGTVTDANLYRYRLLVAEAGSSDFAEFAVGTAPVTNG
                     NLASFDPSLLKNGLYTVLLIAEDINGLQSQDSATIQVEGELKPGVVQLSFVDMTVPVA
                     GIP"
BASE COUNT          414 a          680 c          716 g          422 t
ORIGIN      
        1 gcaatttgcc gcaggcaacc gaggacgtcg gctatgtggc caccattgtc gccaccgatc
       61 ccaatgcggg cgacctgatt gcgtactcgc tggtggccgg tcccaccggc ctggtgattg
      121 acgccgccag cggtcagatc aactggttgc cgcaggataa ccagaccggc agccataccc
      181 tcctggtgcg ggcaacggac agtgccggcc tgttcagcga ggcgagtttc ctgatccagg
      241 ttctgccggt gaatgatgcg ccggtgatta cctcgagccc atccgccagc gcgcaatcac
      301 agagtgcata ccgctatcag gtggcggtgg aggatccgga tgccggcgat acgctcgcct
      361 ttgctttgac gcaggcaccg gcgggcatga ccatcagcag taccggtctg atcgagtggc
      421 tgccgccgtc caacgccgcc gccagctacg cggtgcaggt cacggtcacg gatgccatcg
      481 gcgccagcga cagccagagt ttcagcattt cgctgctcaa ccgcgaaccc gtcatcacca
      541 gcctgccggc ggcaggcgtg cccgaacgtc agttgtacag ctatccggtt caggcccagg
      601 acgccgacgg cgacaccctg agttttgcgc tgcagaacgc accagcatcg atgtttatca
      661 atgcggcgtc cggcttgctg caatggcaga cccaggcggg cgatgaaggc agctacgccg
      721 ttgttgtcac cgttgcggat ggtcgtggcg gtttcgccac acagaatgtg ctggtcacga
      781 tcgatccacc tgccaacacg gcgcctgtga tttccagcac acctgtattg gcagcgaccg
      841 aaggtgcagc ctatgcctac caggtgctgg cgcaggacgg cgaggcggac ccgctttcct
      901 tcgcgctgac cagcgcgccc cagggcatgg tgatgtcagc gggtggactg atcagctgga
      961 cgccggatgc cacccaggtg ggtgaacatg cggtggcggt gcaggtttcg gatggccgtg
     1021 cccaggccag ccagagtttc accctggtgg tgaatgccaa acccaacacg gcgccgcaga
     1081 tcagcagcgt gccggtgatg gcggtgcagg ccaatacggc ctatcaatac caggttgtcg
     1141 caaccgacgt cgacaacgaa gccctgacct tcagcctgcc cactgcaccg gcaggaatga
     1201 gcatctcttc cagcggcctg gtgaactgga cgcccgatga tacccagctg ggtggccatg
     1261 cggtggtggt acgtgtgacg gatgcgcgcg gtgcgtttgt gcagcagagc ttcacgctgg
     1321 cggtgcaggc cgtcgtcaac catcctccgg tgatcagcag tgcgccggtg acggtggcct
     1381 tggtggggca gtcgtacagc tacaccgttg cggccagtga tccggaaaac gcgccgctga
     1441 cctattctct ggtgcaggct ccggcaggca tgtcgatgac catgttcggt gacgtcagct
     1501 ggacaccgct tgcaacgcag gtgggcagcc acgcagtggt ggtgcaggta acggatggca
     1561 ccacacccgt cacgcagaat ttcaacgttg tggtttcgga gccggcagac ctggatgtgt
     1621 ccattcagct gtcggatgat gtggtcgctc ccggcgaggc cgttaccatt caggttgttg
     1681 cagcagatgc gattaatccc gtgtatgcgc tgaccgtgaa tggcagtgca ctcacagtgg
     1741 atggcaatgg cgttgcgcag ttcagctcga cgacctcggg catctatcaa ctgattgccc
     1801 aggtgcaaag cgcggatggt tccagcggaa ccggccagac cgtgctgcgc gtctccgtcg
     1861 ccggcgacac ggtcgcgccc gtagccaata tcattgcccc cgccgatggc acgaccgtgt
     1921 acgacgcgac cgcgattacc gggactgtca ccgatgccaa tctttatcgc tatcgcctgc
     1981 tggtggcgga agcgggctcc agcgatttcg cggagtttgc tgtgggcacc gcaccggtga
     2041 ccaacggcaa tctcgccagc ttcgatccga gcctgctgaa gaacggtttg tacacggtgc
     2101 tgctgattgc ggaagacatc aacggtctgc agagccagga tagcgcgact atccaggtgg
     2161 aaggtgaact gaagccgggt gtggtgcaac tgagctttgt cgacatgacg gtacccgttg
     2221 ccggaatccc ga
//
LOCUS       DASUEG010000799         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_164849, whole genome shotgun sequence.
ACCESSION   DASUEG010000799 DASUEG010000000
VERSION     DASUEG010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164849"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFX11_15060"
     CDS             <1..1114
                     /locus_tag="VFX11_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX4939997.1"
                     /translation="FFIFSVHMMGISSIMGAVNVIVTIFNLRAPGMTLMKMPLFVWTW
                     LITAFLLIAVMPVLAGAVTMMLTDRHFGTSFFDAAGGGDPVLFQHVFWFFGHPEVYIM
                     ILPAFGVVSAIIPTFARKPLFGYASMVYATASIAFLSFVVWAHHMFTVGLPLAGNLFY
                     MYMTMLIAVPTGVKVFNWVATMWKGSMTFEVPMLFAIAFVILFTIGGFSGLMLAITPV
                     DQQYHDTYFVVAHFHYVLVTGAVFSIIAGVYYWLPKWTGRMYSEFWGKIHFWSSLLSV
                     NLLFFPMHFVGLAGMPRRYSDYALQFADFNWWISIGGFWFGLSQLILLKILIDVCFRK
                     KGAQATSEVWEGAEGLEWTLPSPAPYHSFTTPPLVK"
     gene            1123..1701
                     /locus_tag="VFX11_15065"
     CDS             1123..1701
                     /locus_tag="VFX11_15065"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX4939998.1"
                     /translation="MTEQNARAQDSIKLAKRLTLIAIGMFVFAVGILPPMYDVICDIT
                     GLNGKTANEADSAESAVVQEERLITVQFLADTDPAMPWDFRPVERSIKVHPGEIRKVD
                     FHVRNRDAQWLVGQAIPSVTPSQATPYFKKTECFCFAQQELQGGDAKDMPLIFYIDPD
                     LPKNVTTITLSYKLFNITDRVQQESKRVATTQ"
     gene            1738..>2466
                     /locus_tag="VFX11_15070"
     CDS             1738..>2466
                     /locus_tag="VFX11_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4939999.1"
                     /translation="MAQKPTQYYVPDQSPWPVITALALFIVAYGAASFIQQNSGSGLV
                     ANDGSSGKILLPLGLLGIVAMMFFWFRDTIKESLAHKNSAQLDVSYRMGMMWFIFSEV
                     MFFGAFFGALFYVRLLAVPWLGGAGEEAMTHELLWPNFQAMWPLTVTPDGSTTEAMPA
                     WGLPFLNTVLLVSSSVTLTIAHHALLAGNRAKLQINLFITLLLGFAFLFFQVEEYMHA
                     YQEMGLTLDAGIYGSTFFMLTGFHG"
BASE COUNT          463 a          706 c          714 g          583 t
ORIGIN      
        1 ctttttcatc ttctcggtgc acatgatggg gatctcctcg atcatgggtg cggtgaacgt
       61 gatcgtgacg atcttcaacc tgcgcgcacc gggcatgacg ctgatgaaaa tgccgctgtt
      121 cgtctggacc tggctgatca ctgcattcct gctgattgcg gtaatgccgg tgctggcagg
      181 cgcggtgacc atgatgctca ccgaccgcca cttcggcacc agcttcttcg atgcagccgg
      241 cggcggtgat ccggtgctgt tccagcacgt gttctggttc ttcggtcatc ccgaggtgta
      301 catcatgatc ctgcccgcgt tcggcgtggt gtctgcgatc atccccacct ttgcccgcaa
      361 gccgctgttt ggttacgcgt cgatggtgta cgcgacagct tcgattgcgt ttctgtcctt
      421 cgtggtatgg gcgcatcaca tgttcaccgt gggtctgccg ctggcgggca atctgttcta
      481 catgtacatg accatgctga ttgccgtgcc caccggggtg aaggtgttca actgggtggc
      541 caccatgtgg aaagggtcga tgacctttga agtgcccatg ctgtttgcga ttgcgttcgt
      601 gatcctgttc accatcggcg gtttctccgg cctgatgctg gcgatcacac cggtggacca
      661 gcagtaccac gacacctatt tcgtggtggc gcacttccac tacgtactgg tgaccggtgc
      721 agtgttctcg atcatcgccg gcgtttacta ctggctgcca aagtggacag gccgcatgta
      781 cagcgaattc tggggcaaga tccatttctg gagttcgctg ctgtcggtga acctgctgtt
      841 cttcccgatg cacttcgtgg gtctggccgg catgccgcgc cgctattcgg attacgcgct
      901 gcagtttgcg gatttcaact ggtggattag tatcggcggt ttctggtttg gtctgagcca
      961 gctgatcctg ctgaaaatcc tgatcgacgt ctgcttccgc aagaaaggtg cgcaggcaac
     1021 cagtgaagtc tgggaaggtg ccgaaggcct ggagtggact ctgccctcgc ctgcgcccta
     1081 ccacagcttc accacaccgc ccctggtgaa gtgaggccgc ccatgacgga acagaatgcc
     1141 cgcgcgcaag acagcataaa actggctaag cgtctgacgc tgattgccat cggcatgttc
     1201 gtctttgccg tgggcattct gccgcccatg tatgacgtga tttgcgatat caccgggctg
     1261 aacgggaaaa cggcgaacga agcggattcc gcagagtccg ccgtggtgca ggaggagcgg
     1321 ctcatcactg tgcagttcct ggcggacacc gacccggcca tgccctggga tttcaggccg
     1381 gtggagcgca gcatcaaggt gcaccccggc gagatccgca aggtggattt ccatgtgcgt
     1441 aatcgtgatg cccaatggct ggtaggccag gcgattccgt cagtcacgcc gtcacaggcc
     1501 acaccctatt tcaagaaaac cgaatgtttc tgttttgccc agcaggaatt gcagggcggc
     1561 gacgccaagg atatgccgct gattttttat atcgatcccg atctgccaaa aaacgtaacg
     1621 acgattacac tttcctataa gttgttcaac attacggacc gagtgcagca ggaatcgaag
     1681 cgggtcgcca ccacccaata acgtattgcc aacgatttta caaaaaggag atcggtaatg
     1741 gcccagaaac caacacagta ttatgttcct gatcaaagcc catggccggt gatcacggcg
     1801 ctggcgctgt tcatcgtggc ctatggcgcc gcgagtttca tccagcagaa ctccggctcc
     1861 ggactggtgg ccaatgacgg cagctccggc aagatcctgt tgccgctggg actgctgggc
     1921 attgtggcca tgatgttctt ctggttccgc gacaccatca aggaaagcct ggcccacaag
     1981 aacagcgccc agctcgacgt atcctatcgc atgggcatga tgtggttcat tttttcagaa
     2041 gtgatgttct tcggcgcgtt tttcggtgcg ctgttctacg tgcggctgct ggctgtgccc
     2101 tggctgggcg gtgccggcga ggaagccatg acccacgaac tgctgtggcc gaatttccag
     2161 gccatgtggc cgctgactgt aacgccggac ggttctacga ctgaagcaat gcccgcctgg
     2221 ggtctgccct tcctcaacac cgttctgctg gtaagcagca gcgtgacgct gaccatcgcc
     2281 catcatgctc tgctggccgg caatcgcgcc aagctgcaga tcaacctgtt catcaccctg
     2341 ctactgggct tcgcgttcct gttcttccag gtggaagaat acatgcacgc gtaccaggaa
     2401 atggggctga cactggatgc cggcatttat gggtccacct tcttcatgct gacgggtttc
     2461 cacggc
//
LOCUS       DASUEG010000800         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_222097, whole genome shotgun sequence.
ACCESSION   DASUEG010000800 DASUEG010000000
VERSION     DASUEG010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222097"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFX11_15075"
     CDS             complement(<1..673)
                     /locus_tag="VFX11_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940000.1"
                     /translation="MRKPMQSLLGVACLVLAVQAAQAVPINYSFSGTSVGEVSGSGAP
                     VATKIYSGTTSIGGTFTYDDAAPAVGYSPVAGYNNALFTTYSGAFSNMDMTVAGSSAS
                     ADLGVGLVGNNVAITGTINPIDVLLGYSSASEPAAGFSGFTVGNWTLTSLAFVFFNGA
                     NTYLGDDLPDTVSATSNLMELFFTSTIGQVQKVRYVLSSVTEVKPVDVPEPATLSLLA
                     LGTAGL"
     gene            complement(909..>2380)
                     /locus_tag="VFX11_15080"
     CDS             complement(909..>2380)
                     /locus_tag="VFX11_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4940001.1"
                     /translation="GSEPQLQTALREQQTGQAPLQMNSPLGPLLCELLMQPVDASGQL
                     VTVPLVNREGRVLGVLCLLYQSLEDSDYVTGEIRTRLAFIHTLSGFAAVSVETRQLIT
                     LQKRLFTAFIELIASAIDAKSPYTGGHCQRVPVLTEMLARAACKSDQPPFDTFDLNAD
                     EWEALHVASWLHDCGKVTTPEHVVDKATKLEARYDRIHEIRMRFEVAKANAETRYWRA
                     RALSEYPSEEELQHTLQRLDEDFAFVARCNLGGENLAQEDQQRLHEIAQQQWRRTLSD
                     RLGIGWEEAHRKQNIPDSALPVDEPLLADRPDHLIPRDPADALRADRQATFNLIPPQH
                     KYNLGELHNLCIERGTLTDEERYLINDHIVQTILMLEHLPYPEHLRGVPAIAGGHHEK
                     MDGTGYPRGLTATDMPLTARMMAIADIFEALTAGDRPYKAAKTLSESLRIMQRMAKEK
                     HIDPDLFHLFIQSGVYRAYAERFLTQEQMDGVDETGLVG"
BASE COUNT          489 a          706 c          668 g          517 t
ORIGIN      
        1 gcaaaccggc agtacccagc gccagcaacg aaagtgttgc gggctcgggc acatccaccg
       61 gcttaacctc agtaacagaa gacagaacat atcgcacttt ctggacctgc ccgatggtgc
      121 tggtgaagaa caattccatc agattggaag tagcgctgac cgtatcggga agatcatcac
      181 ccagataggt attggccccg ttgaagaata caaacgccaa cgaagtcagc gtccagttgc
      241 caaccgtaaa accgctgaat cccgctgctg gttcactggc tgacgaatag cccagcaata
      301 cgtcgatagg attgattgtg cctgtgatgg cgacattgtt tcccaccagg cccacgccca
      361 ggtcagcgct ggcggacgat ccggcgaccg tcatatccat gtttgaaaac gcgccggaat
      421 aggtggtgaa cagtgcattg ttgtacccgg cgactggcga gtaacccact gcaggtgctg
      481 cgtcgtcata ggtgaaggtg ccaccgatac tggtggtgcc tgaatagatt tttgtggcga
      541 caggggcgcc gcttccgctg acctcgccca cgctcgttcc gctgaatgaa taattgattg
      601 gcactgcctg cgcggcttgc accgccaaca ccagacaggc tacccctaac agactttgca
      661 tcggtttacg catgtgtatc cccatcgatt tgaaggttgc caaacacttt gccatctaaa
      721 aggtgcgcaa ccccacgcac ggcttccctg caagggcatc cttcacacca gtggccaagt
      781 caattaatag aatgaaccag actacttgat aaggcttgga acaagccctc gaatcaagct
      841 tcctgaacaa tactgattat gttctgtact ttcaactggg ttattgtgac aggcccgggc
      901 agcgcccctt aacccaccaa ccccgtctca tccaccccat ccatctgttc ctgcgtcagg
      961 aagcgttccg cataggcacg gtatacgcca ctctggatga acagatgaaa cagatccgga
     1021 tcgatgtgct tttccttcgc catgcgctgc atgatccgca gcgattcgct cagggttttg
     1081 gccgctttgt agggccgatc ccctgcggtc agcgcttcga aaatatcggc aatcgccatc
     1141 atgcgcgctg tcagtggcat gtcggttgcg gtcagcccgc gcgggtaacc ggtgccatcc
     1201 attttctcat ggtggccacc ggcaatggcc ggcacgccgc gcaaatgttc cgggtagggt
     1261 aaatgttcca gcatcaaaat cgtctgcacg atgtgatcat tgatcaggta gcgttcttca
     1321 tcggtcagcg tgccgcgctc gatgcacaga ttgtgcaact cccccaggtt gtatttgtgc
     1381 tgcggtggga tcagattgaa cgtggcctga cggtcggcgc gcagggcatc ggcggggtcg
     1441 cggggaatca ggtggtcggg gcggtccgcc aacaggggtt catccacggg caacgcgctg
     1501 tcgggaatat tctgtttgcg atgcgcttct tcccagccga tacccaggcg atcgctcaat
     1561 gtgcgccgcc attgttgctg agcgatttcg tgcagccgct gctggtcttc ctgcgccagg
     1621 ttctcaccgc ccagattgca gcgggcaaca aacgcgaaat cctcgtccag gcgttgcaat
     1681 gtgtgttgca gctcttcttc gctgggatac tcactcaacg cccgcgcgcg ccagtaacgg
     1741 gtttcggcgt tggctttcgc cacttcaaaa cgcatgcgga tttcgtggat gcggtcatag
     1801 cgcgcttcca gtttggtggc cttgtccacc acatgctccg gcgtggtgac cttgccgcaa
     1861 tcatgcagcc agctcgccac atgcagtgct tcccactcgt cggcattcag gtcgaaagtg
     1921 tcgaacggtg gctggtccga tttgcaggcg gcgcgggcca gcatttccgt cagcaccggc
     1981 acgcgctggc agtggccgcc ggtgtaaggc gatttggcgt cgatggcgct ggcgatcagt
     2041 tcgatgaacg cggtgaaaag gcgtttctgc aaggtgatca gctggcgggt ttcaacactg
     2101 acggccgcga atcccgacaa ggtatgaatg aacgcaaggc gcgtgcgaat ttccccggtg
     2161 acataatcgg aatcttccag tgactgatac agcaaacaca gcacgcccag tacgcggccc
     2221 tcgcggttca ccagcggcac tgtcaccagt tgtccgctgg catccaccgg ctgcatcaac
     2281 aattcacaca gcagcgggcc cagcgggctg ttcatctgca acggcgcctg tcccgtctgc
     2341 tgttcacgca acgccgtctg cagttgaggc tcgctgccgc
//
LOCUS       DASUEG010000801         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_466581, whole genome shotgun sequence.
ACCESSION   DASUEG010000801 DASUEG010000000
VERSION     DASUEG010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466581"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1896
                     /gene="sdhA"
                     /locus_tag="VFX11_15085"
     CDS             124..1896
                     /gene="sdhA"
                     /locus_tag="VFX11_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX4940002.1"
                     /translation="MSNIRTLTFDAVIVGGGGAGMRAALQLAQSGQKTALISKVFPTR
                     SHTVSAQGGITCAIASADPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSVGPQVVFELE
                     HMGLPFSRTDEGRIYQRPFGGQSKNYGEGGQAARTCAAADRTGHALLHTLYQQNVRFN
                     TNFYNEWYAADLVTNQDGAVTGVVAINIETGESVYFKSKATVFATGGAGRIYSSTTNA
                     LINSGDGVGMALRAGFPVQDIEMWQFHPTGIAGAGVLVTEGCRGEGGYLVNKDGERFM
                     ERYAPNAKDLAGRDVVARSMMQEILDGRGCGPEGDHVYLKLDHLGEEVLHKRLPGICE
                     LSITFAHADPVKEPIPVVPTCHYMMGGIPTNINGQAITQGADGVDKIIPGLYAVGEVA
                     CVSVHGANRLGGNSLLDLVVFGRAAGIFIEKALKEGIEHRDSSESDLERAFSRINRLD
                     NSKTGESVAALRKELQTNMQNNFGVFRKGDLMQQGVKKLEELRDRIGKSHLTDKSKSY
                     NTARIEALELDNLLEVAEATAIAAEARKESRGAHCRYDFPDRDDENWLVHSIYFPTDK
                     RVGKREVNFAPKQVPTFKPKVRTY"
     gene            1908..2615
                     /locus_tag="VFX11_15090"
     CDS             1908..2615
                     /locus_tag="VFX11_15090"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX4940003.1"
                     /translation="MLQVSIYRYNPDTDAEPYMKDYQVDTGGKDLMVLDVLALVKEKD
                     DSLAYRRSCREGVCGSDGMNINGKNGLACITPVSQATKGKGKLILRPLPGLPVIRDLV
                     VDMTLFYNQYEKIKPYVVNNTPAPAIERLQTPAERDKLDGLYECILCACCSTSCPSFW
                     WNPDKFIGPAGLLQAYRFIADSRDTMTQERLSALDDPFSLFRCRGIMNCVSVCPKGLN
                     PTKAIGHIRSLLLQQGT"
     gene            2945..>3612
                     /locus_tag="VFX11_15095"
     CDS             2945..>3612
                     /locus_tag="VFX11_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX4940004.1"
                     /translation="MDEGLMYRQWSTSHLFGANAAYIEELYESYLKDPNSVPEQWRDH
                     FDKLPRVNGTEQDIPHSTVREHFLYLAKNRNRAQPLVVSSVSSEHEKKQVKVLQLITG
                     YRVRGHQKANLDPLGLMDRESVPDLELTHHGLSAADLDTTFQTGNVFIGKEEATLREI
                     VEALERTYCTSIGVEYMHIVDTAEKRWIQQRMESVRSHPNYSRDVKIHLLERLIAAEG
                     LEKLL"
BASE COUNT          780 a          989 c         1070 g          773 t
ORIGIN      
        1 attaccaccc gccagctggg tgccattgca accccgttgc ggattgcggt tcaggccgga
       61 tttgtgctgg tgacgatcgt gtatgtgatc tggggcgccc agattttgtg gggaatttaa
      121 aacatgtcga acattcgtac cttgaccttt gatgcggtga tcgtgggtgg tggcggcgcc
      181 ggtatgcggg cagctcttca gctggcccaa tccggacaga agaccgcgct gatctccaaa
      241 gtgtttccta cccgttccca caccgtatcc gcccagggcg gtattacctg cgcgattgcc
      301 agtgccgatc ccaatgatga ttggcgctgg cacatgtacg acaccgtaaa ggggtctgac
      361 tatatcggtg accaggacgc catcgaatac atgtgcagtg tcggccccca ggtggtgttc
      421 gagctggagc acatgggtct gccgttctcc cgaaccgatg aaggccgtat ttaccagcgt
      481 ccgttcggcg gtcagtccaa gaattacggt gaaggcggtc aggcagcgcg tacctgcgcg
      541 gctgcggacc gtaccggtca cgcgctgctg cacaccctgt atcagcagaa cgtccgtttc
      601 aacaccaact tctataacga atggtatgcc gccgatctgg ttaccaacca ggatggcgcg
      661 gtgacaggcg ttgttgccat caacatcgaa accggtgaat cggtttactt caaatccaaa
      721 gcgactgtgt tcgctactgg cggagcaggg cggatctatt cgtccaccac caacgccctg
      781 attaactcgg gtgacggcgt gggcatggca ctgcgtgccg gtttcccggt gcaggacatc
      841 gagatgtggc aattccaccc gaccggtatt gccggcgcgg gtgttctggt caccgagggt
      901 tgccgcggtg aaggcggtta cctggtgaac aaggacggcg agcgtttcat ggagcgttat
      961 gctccgaatg cgaaggacct tgccggtcgt gacgtagtcg cgcgttccat gatgcaggaa
     1021 atcctggatg gccgtggttg cggtccggaa ggcgatcacg tttacctgaa gctggaccat
     1081 ctgggcgagg aggtgctgca caagcgcctg cccggtatct gcgaactgtc gatcaccttt
     1141 gctcacgccg acccggtgaa agagccgatt ccggtggtgc cgacctgcca ttacatgatg
     1201 ggtggcattc ccaccaacat caacggccag gcgattaccc agggtgccga tggcgtcgac
     1261 aagatcattc ctggcctgta tgccgtgggc gaagtggcct gcgtatccgt gcatggtgcc
     1321 aaccgtctgg gcggcaactc gctgctggat ctggtggtat tcggacgagc agcgggcatc
     1381 ttcattgaga aggcactgaa ggaaggcatt gaacaccgcg attccagcga gtccgatctg
     1441 gagcgggcat tcagccgtat caaccgcctg gataacagca agacgggtga gagcgtggct
     1501 gctctgcgca aggaattgca gacgaacatg cagaacaact tcggcgtgtt ccgcaagggc
     1561 gacctgatgc agcagggtgt gaagaagctg gaagagctgc gcgatcgcat cggcaaatct
     1621 cacctgacag acaagagcaa gtcctacaac accgcccgta tcgaggcgct ggaactggat
     1681 aacctgctgg aagtggcgga agccaccgcg atcgcggcgg aagcgcggaa agagagccgt
     1741 ggcgctcact gccgctatga cttcccggac cgtgatgacg agaactggct ggtgcactcc
     1801 atctatttcc cgaccgacaa gcgggtcggc aagcgtgaag tcaacttcgc tcccaagcag
     1861 gttccgacct tcaagcccaa agtcagaaca tactaaggtg attcgacatg ttgcaggtta
     1921 gtatttatcg ctacaacccg gataccgacg ccgaacctta catgaaggat taccaggtcg
     1981 ataccggtgg caaggatctg atggtgctgg acgtgctggc actggtaaag gaaaaggacg
     2041 attcgctggc ctatcgccgt tcctgccgtg aaggcgtgtg cggttccgat ggcatgaata
     2101 tcaatggcaa gaacggcctg gcgtgtatca cgccggtgtc gcaggcgacc aaaggcaagg
     2161 gcaagctgat cctgcgcccg ctgcccggtc tgccggtgat tcgcgatctg gtggtggaca
     2221 tgaccctgtt ctacaaccag tacgagaaga tcaagcccta tgttgtgaac aacacgcccg
     2281 caccggccat cgagcgtctg cagacgccgg cggagcgcga caagctggat ggtctgtacg
     2341 agtgcattct gtgcgcctgc tgctccactt cctgcccgtc gttctggtgg aacccggaca
     2401 agttcatcgg tccggccggt ttgttgcagg cttaccgctt cattgcggac agtcgtgaca
     2461 ccatgacaca ggaacgtctg tctgcgctgg atgatccgtt cagcctgttc cgttgccgtg
     2521 gcatcatgaa ctgtgtgagc gtttgcccga aaggcctgaa ccccaccaag gctatcggcc
     2581 acatccgttc cctgttgttg caacagggca cttaaaagct tgtcagcaaa ggaaaaatca
     2641 ctataattct ggcgtcttgc cgcccaagcg ttccctttgg gcggcgtttt gcaaagacgc
     2701 tacacttgat gttgggaatg acagggccgt ccaagaacta atgttgggac gagctttgtt
     2761 ggtttactcc cccgctcaaa aagcggttgt gagtctgttt ataaccaaag ggctatcggt
     2821 aaaaccgaac tatctggaag cgttgttggc ctaaccggct tgtcgcaatc ctaacatgtt
     2881 tgaacgtgtc tgttgctgat tcgcgtcttg cagatagtgt cccgattcga cacggtgatc
     2941 tgcaatggat gaaggtttga tgtatcggca gtggagtacg tcccatttgt tcggggcgaa
     3001 tgccgcctat atcgaagagc tgtacgagtc ctatctgaag gacccgaatt cggtgccgga
     3061 gcagtggcgc gaccacttcg acaagctccc ccgcgtaaat ggcactgagc aagacattcc
     3121 ccactcgacc gtccgggaac attttctata cctcgccaag aatcggaatc gcgcacaacc
     3181 gctggttgtc agcagcgttt cgtccgagca tgagaaaaag caggtcaagg ttctgcagct
     3241 gatcaccggc taccgtgtgc gtgggcacca gaaagccaat cttgatccgc tgggtctgat
     3301 ggatcgtgaa tctgtcccgg atcttgagct gactcatcat ggcctctctg ccgcggatct
     3361 ggataccaca ttccagaccg gtaatgtttt catcggcaag gaagaagcca ctctccgcga
     3421 aatcgtcgag gcactggagc gcacctattg caccagcatc ggcgtcgaat acatgcatat
     3481 tgtggacacc gcagagaagc gctggattca gcagcgcatg gaaagcgtgc ggtctcatcc
     3541 gaattacagc cgcgacgtaa aaatccacct gctggagcgg ctgattgcgg cggaaggtct
     3601 ggaaaaactg ct
//
LOCUS       DASUEG010000802         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_871688, whole genome shotgun sequence.
ACCESSION   DASUEG010000802 DASUEG010000000
VERSION     DASUEG010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871688"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..455)
                     /locus_tag="VFX11_15100"
     CDS             complement(99..455)
                     /locus_tag="VFX11_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940005.1"
                     /translation="MNLRRVLCSGICLMVLGACASSGHQHSTSVHVGVGYHDPWYHGG
                     YYYGNYPPAVMVPPPYPGRPPGSRPPGERPPIDGRPPIDARPPVAKPPIAKPPVSRPP
                     SIPSRPRPMPMRGGRR"
     gene            complement(452..1303)
                     /locus_tag="VFX11_15105"
     CDS             complement(452..1303)
                     /locus_tag="VFX11_15105"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4940006.1"
                     /translation="MKPTRLQLRFLLFTLLTLASTPLTADPTRLPGKFVWADLVTHDA
                     ASAQQFYHAWMGWEFRPAGDVQLITLQDRTIGALRQRPQTQDSPARPRWIGYVSVSAV
                     DAASKRVIAMGGRVLLPQQNDALHGDFALVTDAEDALFGLIRRPDGDPEDYLAEPGEW
                     IWMQLFSRDPTKAAAFYQPVGDYQLFPLDDAGHRQLLVKDGYSRAALSRLPETQPDLK
                     PAWLPFLRVKDVAQAANHAAALGGKILLAPDPARVAGRVAIVADPTGAAVGILEWHDD
                     GNAEVTP"
     gene            1544..2689
                     /locus_tag="VFX11_15110"
     CDS             1544..2689
                     /locus_tag="VFX11_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX4940007.1"
                     /translation="MSSLILLPRILHIGAGASAQVGDVLKTLGCSRPLIVTDASMVKL
                     GYVERLQAFLPPSDVYADTVPEPTEASIIAGVEKVRHGQYDCIIALGGGSPIDSAKAI
                     AILGKHGGRMRDYKVPRIVNEPGLPVIAIPTTAGTGSEATRVTVITDDERDEKMLCLG
                     MGFLPVAALVDFELTLSLPPRTTADTGIDALTHAIEAYVSRKANAYTDSQALQAMQLI
                     APNLRRAFHNGQDRDAREAMMLGATLAGIAFSNASVALVHGMSRPIGAFFHVPHGLSN
                     AMLLPSVTAYSLPAATARYADCARAMRVASVSDSDAMANRKLLDELVALNAELRVPTP
                     AAFGIDRATFFNLTSIMAEQALASGSPGNNPRVPTVDEMVKLYEQLW"
BASE COUNT          537 a          860 c          849 g          535 t
ORIGIN      
        1 cagtacggcg aagccgggaa ttgattaccg ctgacagacc ctgatccagt cccaaacgaa
       61 aaagccgcca aagttggcgg ctttttcgta tatcggcttt agcgccgtcc tccccgcatg
      121 ggcatggggc gcggtcgcga aggaatcgat ggcggtctgg agaccggtgg ttttgcaatg
      181 ggtggtttcg ctaccggcgg tctcgcgtcg atgggcggcc gtccatcgat cggaggacgt
      241 tcgccaggag gccggctacc cggcggcctt ccggggtaag gcggtggcac cattaccgca
      301 ggcgggtagt tgccgtagta gtaaccgccg tgataccagg gatcgtgata accgacaccc
      361 acgtgaacgc tggtgctgtg ctggtggccg ctggaagcgc aggcgcccag caccatcagg
      421 catattccgc tgcacaggac gcgacgcagg ttcatggcgt cacctccgca ttcccatcgt
      481 cgtgccattc cagaataccc acggcggcac cggtcggatc ggccacaatg gctaccctgc
      541 cagccacccg cgcgggatcg ggcgccagca gaatttttcc acccagggcc gccgcgtggt
      601 tcgcggcctg cgcgacatcc ttcacccgca ggaacggcag ccacgccggc ttcagatccg
      661 gctgcgtttc cggcagacga ctgagcgcgg cgcgggagta accgtctttc accagcaatt
      721 gccggtggcc ggcgtcgtcc agcggaaaca attgatagtc acccaccggc tgatagaacg
      781 cggcggcttt cgtggggtca cggctgaaca gctgcatcca gatccattcg cccggttccg
      841 ccagataatc ctcaggatca ccatccggcc gccggatcag cccgaacagg gcatcttccg
      901 catcggtcac caacgcgaaa tccccgtgca gggcatcatt ctgttgcggt aacaatacgc
      961 ggccgcccat ggcgatgacc cgcttgctgg ccgcatccac cgccgacacc gacacatagc
     1021 cgatccagcg cggacgagcg gggctatcct gcgtttgggg gcgctggcgc aaggcgccga
     1081 tggtgcggtc ttgcagcgta atcaattgca cgtcgccggc ggggcgaaat tcccagccca
     1141 tccaggcgtg gtagaactgt tgtgccgacg ccgcatcgtg cgtcaccaga tcggcccaga
     1201 cgaatttgcc gggaaggcgc gtgggatcgg ccgtcagcgg ggtgctggcc agtgtaagca
     1261 gagtgaagag cagaaagcgt aattgaagcc tggttggttt catgggattc cgttgtcagg
     1321 gctcaccgcc cttcaggtat tgcgctatgg atttgattct ggccgtaata atcaaatttg
     1381 gcgaggtctg gtcgtcacct gattgttcgt tgagtggata gtatgtcatt ggcaatttca
     1441 ataatttcat cacccggctg cagagtgccg ttcgctccaa actgtgctct aatccggatg
     1501 cggatttcgc aaagccacca acctacacag cgagcacgtc accatgagca gcctgatcct
     1561 gcttccccgt attcttcata tcggcgccgg cgcatcggct caggtgggcg acgttttaaa
     1621 aacgttgggt tgttcccgcc ctttgatagt caccgacgcc agcatggtga aactgggcta
     1681 tgtggaacgg ctgcaggcgt ttctgccccc cagcgacgtc tatgccgaca ccgtgccgga
     1741 acccaccgaa gcctcgatca tcgccggcgt ggaaaaagtg cggcacggac agtacgactg
     1801 catcatcgcc ctgggcggcg gcagccccat cgacagcgcc aaagccattg cgattctggg
     1861 caagcacggt ggccgcatgc gcgattacaa ggtgccgcga atcgtcaatg aaccgggcct
     1921 gccggtgatt gcgattccca ccaccgccgg caccggttcc gaagccaccc gcgtcaccgt
     1981 catcaccgac gatgaacgcg acgaaaaaat gctgtgtttg ggcatgggat ttctgccggt
     2041 ggccgcactg gtggatttcg aactcaccct cagcctgccg cctcgcacca cggccgacac
     2101 cggcatcgac gcactcacgc acgccatcga agcctatgtg tcgcggaaag cgaatgccta
     2161 caccgacagc caggcgctac aggcgatgca actgattgcg ccgaatctgc gtcgcgcttt
     2221 tcacaatggt caggatcgtg atgcgcggga agccatgatg ctgggtgcca ctctcgccgg
     2281 cattgcgttt tccaatgcgt cggtggcgct ggtgcacggc atgagccgtc cgataggtgc
     2341 gtttttccat gtgccgcacg gcctgtcgaa cgcgatgctg ttaccctcgg tcaccgccta
     2401 ttcgcttcct gcggccactg cccgctatgc ggattgcgcg cgggcgatgc gggtggccag
     2461 cgtcagtgac tccgacgcaa tggccaatcg taaattgctg gatgaactgg tggcgctcaa
     2521 cgcggagctg cgcgtgccga caccggcagc gttcggcatc gatcgggcaa ccttcttcaa
     2581 cctgacctcc atcatggcgg aacaggcgct ggcatcagga tcgccgggca acaatccccg
     2641 tgtgcccacg gtggacgaga tggtgaagct gtacgaacag ctttggtgat gaagtgcttt
     2701 gttgatagtt agggggccaa aagtgcaggc cgccagcgaa ttcgcccgcg ccaactctaa
     2761 atagaaagcc gccttggcag g
//
LOCUS       DASUEG010000803         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1644981, whole genome shotgun sequence.
ACCESSION   DASUEG010000803 DASUEG010000000
VERSION     DASUEG010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644981"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="VFX11_15115"
     CDS             <1..2190
                     /locus_tag="VFX11_15115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX4940008.1"
                     /translation="ELQQPFVHSPDSTHREITLMVNGITCAACVWLVEQNLNRLDGVE
                     QCQVNLSSHRARVVWNPGSTSLSRIIRAIAQVGYKAEPYNPVQQEDIIKRENRQALLR
                     MGLAGLGAMQVMMYAAGLYWGAWEGIDPDHVVFLRWVSGVVCTPVFFYSCVPFYQNAW
                     RALKHRHLNMDVPVSLALVIAYVASWWSVFFQGPEVYFDSVSMFAFFLLLGRYLEMRV
                     RHRSQRTSIRLSHSSIQTARKLDAGGNPQLVPAEKLRKGDRVLVRAGEQIPGDGRIVE
                     GSSSVNESMLTGEHLPTPKMPGDEVTGGTLNIEQPLVLEIQRDPQESTLSTLRRLLDR
                     AESEKPSTTLVADRISSQFVAALLIISVAVFAFWWFHQPERAFWIVLAVLVVTCPCAL
                     SLATPTAITAATAALANNGFLSTRAHTIEGLRYITDVVFDKTGTLTEGRFSLVETRLL
                     GGDSTAQVEGLAAALEQASEHPIARAFHPLTASGDVASIRVIPNEGVHGIWNGAEIRI
                     GKPGFAGALLASHPAAPDAGQWVLLATAEKALAWFRVEDRLREDAATLVQQLQQTGKR
                     CHILSGDGSGHAREIGRQLGIADVRDNASPQAKLDYLKQLQAGGAQVLMLGDGLNDAP
                     VLAGANVSIAMSDGTDLAKVAADGVLLGQSLSPLLQVLTIVSQTYRIIQQNLAWAIGY
                     NLIALPVASVGLIPPWLSALGMSASSLIVVMNALRINRHGQRTTTDR"
     gene            2199..2393
                     /gene="ccoS"
                     /locus_tag="VFX11_15120"
     CDS             2199..2393
                     /gene="ccoS"
                     /locus_tag="VFX11_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011622776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX4940009.1"
                     /translation="MDVIYFLIPLALVLLVAAVWAFFWAVKHDQFEDLDREANRILFD
                     EDIARPKKPNPTGQQEPPQG"
     gene            2397..3095
                     /locus_tag="VFX11_15125"
     CDS             2397..3095
                     /locus_tag="VFX11_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010634023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4940010.1"
                     /translation="MDPSFDFIAAFAIGFMGSLHCIGMCGGISSALTTAVTPAQHSLG
                     RQLGYPLTYNLGRIASYSIAGAIAGVLGSQFRDLLGGHGPSTLRIFAAVMMILLGLYL
                     SGWWKVLNHLERAGTRVWKKLAPLTRHFIPVNNPFKALLLGMLWGWLPCGLVYSALAW
                     SVGAGDAMSGALLMLYFGLGTLPAMLGVGVFSHLLNDIARSQTTRTFAALLLIAFGIW
                     TLVSQIPMPGGHHH"
     gene            3208..>4331
                     /locus_tag="VFX11_15130"
     CDS             3208..>4331
                     /locus_tag="VFX11_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX4940011.1"
                     /translation="MSDHVVENFICTLPWRIGNTIELLVDGDQFFPRILDACAQARHS
                     IDIEMYLFESGQIANRFISTLSAAVERGVRVRLLLDHFGSYHLDQQDVLLLRQRNIDL
                     RFFNRVERHKMVRNLSRDHRKIITVDQQLAFVGGAGITDAFSPQVSGRRAWRELMVEI
                     RGPVVNDCQILFERTWANHDAVHTEHLRERILHWRQPVYNAHHMNRSISQARVNATRG
                     MGNKPIMGALLSEMKKAESCIWISTAYFFPARKLVNLLRKAARNGIDVRLLLPGPHTD
                     HPGVRFAGRSWYKMLLKDGVRVYEYQPRFLHMKLALVDDWCSLGSCNFDRWNLRWNLE
                     ANLEVIDHEFASQVEAMLVNDFTQSVEYTHERWKQRGLLE"
BASE COUNT          779 a         1388 c         1338 g          826 t
ORIGIN      
        1 gaactgcagc agccgttcgt gcattcgcct gactccaccc accgcgaaat caccctgatg
       61 gtgaacggca ttacgtgtgc cgcctgcgtc tggctggtgg aacagaacct gaaccggctg
      121 gatggcgtcg agcagtgcca ggtgaacctg agcagccatc gcgcccgcgt ggtgtggaat
      181 cccggcagca ccagcctgag ccggatcatc cgcgccattg cccaggtggg ctacaaggcc
      241 gagccctaca acccggtgca gcaggaagac atcatcaagc gcgagaaccg gcaggcgctg
      301 ctgcgcatgg gtctggcggg cctgggtgcc atgcaggtga tgatgtacgc cgcaggactg
      361 tactggggcg cctgggaagg catcgatccg gatcacgtgg tgttcctgcg ctgggtcagc
      421 ggtgtggtct gcaccccggt gtttttctac tcctgcgtgc cgttttacca gaacgcctgg
      481 cgcgcgctga aacaccgtca tctgaacatg gatgtgccgg tgtcgctggc gctggtgatc
      541 gcctatgtcg ccagctggtg gtcagtgttt ttccaggggc cagaagtgta tttcgattcg
      601 gtgtcgatgt ttgcgttttt cctgctgctg ggacgctacc tggaaatgcg ggtacggcac
      661 cggtcgcagc gcacctcgat ccggctgtcc cactccagca tccagaccgc gcggaaactg
      721 gatgccggtg gcaatcccca gctggtgccg gccgaaaaac tgcgcaaggg cgaccgcgtg
      781 ctggtgcggg ccggcgagca gattcccggc gatggccgca ttgtggaagg gtccagttcg
      841 gtcaacgaat ccatgctcac cggcgagcat ttacccacgc cgaaaatgcc gggggacgaa
      901 gtcaccggcg gcaccctgaa tatcgagcag ccgctggtgc tggagatcca gcgagatccg
      961 caggaaagca ccctgtcgac cctgcgccgc ctgctggacc gggccgaatc ggaaaagccc
     1021 tccaccacgc tggtggcaga ccgtatctcc agccagttcg tggcggcact gctgatcatt
     1081 tcggtggccg tgtttgcatt ctggtggttc caccagccgg agcgggcctt ctggatcgtg
     1141 ctggcggtgc tggtggtgac ctgcccctgc gcactgtccc tcgccacgcc cacggcgatc
     1201 acggcggcca cagcggcact ggccaacaat ggtttcctct ccactcgcgc ccacaccatc
     1261 gaaggactgc gctacatcac ggatgtggtg ttcgataaaa ccggcaccct caccgaaggc
     1321 cgtttcagtc tggtggaaac ccgtttgctg ggcggggatt ccaccgcgca ggtggaaggg
     1381 cttgcggcgg cactggaaca ggcgtcggaa catcccattg cccgcgcgtt tcatccgctg
     1441 acggccagcg gcgatgttgc gagtattcgc gtgattccga atgaaggtgt gcacggtatc
     1501 tggaatggcg ccgagatccg catcggcaag cccgggttcg ccggtgcgct gctggcaagc
     1561 catcccgccg cgcccgatgc cggccagtgg gtgttgctgg ccacagccga aaaagcgctc
     1621 gcctggttcc gggtggaaga ccgcctgcgg gaagacgccg ccacgctggt gcagcaactg
     1681 cagcagaccg gcaagcgctg ccatatcctg agcggcgacg gcagcggcca cgcccgcgag
     1741 atcggccggc agctgggcat tgccgacgtg cgcgacaatg cctcgccgca ggccaaactg
     1801 gattacctga aacagctgca ggccggcggc gcccaggtac tgatgctggg ggacggcctc
     1861 aatgacgcac cagtgctggc aggggccaac gtgtccatcg ccatgtccga cggcaccgat
     1921 ctggccaagg tggccgccga cggcgtattg ctgggccaga gcctgtcgcc gctgctgcag
     1981 gtgctcacca tcgtcagcca gacctatcgc atcatccagc agaacctggc ctgggccatc
     2041 ggctacaatc tgattgcgct gccggtggcg tcggtcgggc tgattccgcc ctggctgtcg
     2101 gcgctgggca tgtccgccag ctccctgatc gtggtgatga atgcgctgcg catcaaccgc
     2161 cacggccaac gcaccaccac tgacaggtaa cacgcatcgt ggatgtgatc tactttctga
     2221 ttcctctggc cctggtgctg ctcgtggcag cggtgtgggc gtttttctgg gccgtaaagc
     2281 acgaccagtt cgaggatctg gatcgcgaag ccaaccgcat cctgttcgac gaggacatcg
     2341 cccgcccgaa aaagccaaac ccgaccggcc agcaggaacc acctcagggc tgaagcatgg
     2401 acccgtcttt cgatttcatc gccgcattcg caatcggttt catgggcagc ctgcactgca
     2461 tcggcatgtg cggtggcatc agcagtgcgc tgaccacagc ggtgacaccc gcgcagcaca
     2521 gcctgggccg acagctcggc taccccctca cctataatct gggccgcatt gccagctaca
     2581 gcattgccgg cgccattgca ggcgtgctgg gcagccagtt ccgcgatctg ctgggcgggc
     2641 acggaccgtc gacgctgcgg attttcgccg ctgtcatgat gatcctgctg ggcctgtacc
     2701 tgagcggctg gtggaaagtg ttgaaccacc tggaacgggc cggcacccgg gtctggaaaa
     2761 aactggcgcc gctcacccgc catttcattc ccgtgaacaa tccgttcaag gcgctgctgc
     2821 tgggcatgct gtggggctgg ttgccctgcg gcctcgtgta cagcgcgctg gcctggtcgg
     2881 taggtgcggg cgatgcgatg tcgggcgcac tgctgatgct gtatttcggt ctgggcaccc
     2941 tgcccgccat gctgggcgtg ggcgtattct cccacctgct gaacgacatc gcccgctcgc
     3001 agaccacccg caccttcgcc gctctgctgc tgatcgcctt cggcatctgg acgctggtca
     3061 gccagattcc catgccaggc ggccaccacc actgaacgat tgacggcacc ggcttgcccc
     3121 gccccggtgc tttcttttac agtgcatcat cagagtgcaa acaatcgtgt gtccatcgcc
     3181 gatccagcag ggattccagc gtcgctcatg tccgaccatg tcgtggaaaa tttcatctgc
     3241 acgctgccct ggcgcatcgg caacaccatc gagctgctgg tggatggcga ccagtttttc
     3301 ccccgcattc tcgatgcctg tgcgcaggcc cgtcacagca tcgacatcga aatgtacctg
     3361 ttcgaatccg ggcaaatcgc caaccgtttc atttccaccc ttagcgcggc cgtcgaacgc
     3421 ggtgtacgcg ttcgcctgct gctggatcac ttcggctcgt accacctgga tcagcaggac
     3481 gtgctgctgt tgcggcagcg caatatcgac ctgcgctttt tcaaccgggt ggagcgtcac
     3541 aagatggtcc gcaacctgtc gcgggaccat cgcaagatca tcaccgtcga ccagcagctg
     3601 gcgttcgttg gcggtgccgg cattaccgac gcgttttcac cgcaagtttc cggccggcgt
     3661 gcctggcgcg aactgatggt ggaaatccgt gggccagtgg tgaatgactg ccagatcctg
     3721 ttcgaacgca cctgggccaa ccacgacgcc gtgcacaccg agcacctgcg ggagcgcatc
     3781 ctgcactggc gccagccggt ctacaacgca catcacatga accggtcaat ttcccaggcc
     3841 cgcgtcaacg ccacccgcgg tatgggcaac aaacccatca tgggcgcgct gctgagcgag
     3901 atgaagaaag ccgaaagttg catctggatc agtaccgcgt atttttttcc cgcccgcaag
     3961 ctggtgaacc tgctgcgcaa ggccgcgcgc aacggcattg atgtgcgcct gctgctgcct
     4021 gggccacaca cggatcatcc cggcgtgcgc ttcgccggcc gttcctggta caaaatgttg
     4081 ctgaaggacg gcgtgcgcgt ttacgaatac cagccccgct tcctgcacat gaaactggcg
     4141 ctggtagatg actggtgcag cctcggctcc tgcaatttcg accgctggaa cctgcgctgg
     4201 aatctggaag ccaacctcga agtcatcgat cacgagttcg ccagccaggt ggaagccatg
     4261 ctggtaaatg atttcacgca atccgtggaa tatacgcatg aacgctggaa gcagcgcggc
     4321 ctgctggaaa a
//
LOCUS       DASUEG010000804         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1072224, whole genome shotgun sequence.
ACCESSION   DASUEG010000804 DASUEG010000000
VERSION     DASUEG010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072224"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VFX11_15135"
     CDS             complement(<1..99)
                     /locus_tag="VFX11_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4940012.1"
                     /translation="MKILVTGAAGFIGAALSHRLLDRGDDVIGLDNL"
     gene            complement(174..2195)
                     /gene="uvrB"
                     /locus_tag="VFX11_15140"
     CDS             complement(174..2195)
                     /gene="uvrB"
                     /locus_tag="VFX11_15140"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX4940013.1"
                     /translation="MSTERKFKVHANYKPAGDQPAAIEALVEGLNDGLSHQTLLGVTG
                     SGKTFTIANVVEAVQRPTLVMAHNKTLAAQLYGEFKEFFPDNAVEYFVSYYDYYQPEA
                     YVPSSDTFIEKDSSVNEHIEQMRLSATKALLERPDTIIVATVSAIYGLGDPGSYLQMV
                     LHLDRGERMDQRAILRRLAELQYTRNDTEFYRANYRVRGELIDVFPAESEREALRIEL
                     FDDEIENLSLFDPLTGEILQRIPRYTIYPKSHYVTPRENILAAIENIKVELRDRLQFY
                     LDEGKLLEHQRLSERTRFDLEMMQELGYCTGIENYSRYLSGRAPGEPPPTLFEYLPKN
                     ALVVIDESHATVPQIGAMYKGDRSRKETLVQYGFRLPSALDNRPLRFEEWEALCPQTI
                     FVSATPAQYEATHAQQVVEQIARPTGLVDPELEVRPATTQVEDLLSQIHARVAKEERV
                     LVTTLTKRMAEDLTDYLLEQGVKVRYLHSDIDTVERVEIIRDLRLGEFDVLVGINLLR
                     EGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYADRITDSMRRAM
                     DETERRRTKQIEFNKAHGITPKGIRKSVEDIMEGARAPGGKKGQQRKVAEAPSKYAVA
                     PELMEPKQAAALLKELEQKMFHHAKNLEFEQAARLRDEIQRLKNEVFIH"
     gene            2398..>2581
                     /locus_tag="VFX11_15145"
     CDS             2398..>2581
                     /locus_tag="VFX11_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940014.1"
                     /translation="MLATGFLPDPDQVCEGSPPFTLKLQKQVEREETAMTLQREQMSS
                     VDTAWLRMESPVSHMTI"
BASE COUNT          478 a          715 c          818 g          570 t
ORIGIN      
        1 gaggttgtcc agcccgatga cgtcatcgcc acgatccagc agccggtggg acagggctgc
       61 tccgatgaag ccggcggcgc ctgttaccag aatcttcatg cactctcctt aaagggattc
      121 aaccacatac accggtgaag gttatcacaa agcctccatc tgcgggtctg gggttagtgt
      181 atgaaaacct cgtttttgag gcgctggatt tcgtcacgca ggcgggcggc ctgctcgaac
      241 tccagattct tggcatgatg gaacattttc tgctccagtt ctttcagcag tgcggcggcc
      301 tgcttgggtt ccatcagttc cggggccacc gcatatttcg atggtgcctc ggctaccttg
      361 cgctgctggc ccttcttgcc gccgggtgcg cgggcgcctt ccatgatgtc ctcgaccgat
      421 ttgcggatgc ccttgggcgt gatgccgtgg gctttgttga actcgatctg cttggtgcga
      481 cggcgttcgg tttcgtccat ggcgcggcgc atggaatcgg taatgcggtc ggcgtagagg
      541 atggccttgc cattgagatt gcgggcggcg cggccgatgg tctggatcag ggagcgttcg
      601 gagcgcagga agccttcctt gtcggcatcc aggatcgcca ccagggacac ctccggcatg
      661 tcgagacctt cccgcagcag gttgatgccc accagcacgt caaactcgcc caggcgcagg
      721 tcgcggatga tctcgacccg ttccaccgtg tcgatgtcgg aatgcaggta gcgcaccttg
      781 acgccctgct ccagcaggta atcggtaagg tcttccgcca tccgcttggt gagggtggtg
      841 accagtaccc gctcctcttt cgccacacgg gcatggatct gcgacagcag gtcctccacc
      901 tgggtggtgg cggggcgcac ttccagttcg gggtccacca ggccggtggg acgggcgatc
      961 tgctccacca cctgctgggc gtgagtggct tcgtactgtg ccggagtggc ggagacaaaa
     1021 atggtttgcg ggcagagggc ttcccattcc tcgaaacgca ggggccggtt atccagggcg
     1081 gacggcaggc ggaacccata ttgcaccagg gtttccttgc gtgagcggtc gcccttgtac
     1141 atggcgccga tctgcggaac tgtggcgtgg gattcgtcga tcaccaccag cgcatttttc
     1201 ggcaggtatt cgaacagggt cggcggcggt tcgcccggcg cacggccgga cagatagcgc
     1261 gagtagtttt cgatgccggt gcaataaccc agttcctgca tcatttccag gtcgaaacgg
     1321 gtgcgctcgc tgagacgctg gtgttccagc aatttgcctt cgtccaggta aaactgcagg
     1381 cggtcgcgca gttcgacctt gatgttctca atggccgcga ggatgttttc ccgcggcgtg
     1441 acgtaatggg acttgggata aatcgtgtag cgcggaatcc gttgcaggat ttcgccagtg
     1501 agcggatcga acagggacag gttttcgatt tcgtcgtcga acagttcaat acgcagcgct
     1561 tcgcgctcgg attccgccgg gaagacgtcg atcagttcgc cacgcacgcg gtaattggcg
     1621 cggtagaact cagtgtcgtt acgggtgtat tgcagttctg ccagccggcg caggatggcg
     1681 cgctggtcca tgcgctcgcc gcggtccagg tgcagcacca tctgcaggta gctgccggga
     1741 tcgcccagac catagatggc agacaccgtg gcgacaataa tggtgtcggg ccgttccagc
     1801 aacgccttgg tggcggacag gcgcatctgt tcgatgtgtt cgttcaccga ggaatctttc
     1861 tcgatgaagg tgtcggacga gggcacgtag gcttcgggct ggtagtagtc gtagtaggaa
     1921 acaaagtact cgaccgcgtt gtcaggaaag aattccttga actcgccgta cagctgcgcc
     1981 gccagggttt tgttgtgggc catgaccaac gtcgggcgtt gcaccgcctc caccacattg
     2041 gcgatggtga aggttttgcc ggagccggta acgccgagga gggtctggtg tgacaggcca
     2101 tcgttgaggc cttccaccag tgcttcaatc gcagcgggct ggtcgccggc cggtttgtaa
     2161 ttggcgtgaa ccttgaattt gcgctcggta ctcatgatgg ggccatcggg tcaggttaat
     2221 gcatcggcat cggcggattg aaccctgaat gatcccaaac ttcgttcctg cagcacaact
     2281 gacattcact ggcgggcaac cacttgtaat ccaaggcatt tgtattcatt ccgtccgaag
     2341 gtcagaaatg ctggttgcca gacggctgcg cgatgcttgc acgcacgacc tgaatccatg
     2401 ctggcaacag ggttcttgcc tgacccggat caagtctgtg agggcagccc tccctttaca
     2461 ctgaaactgc aaaaacaagt agaaagggag gaaacggcca tgaccttgca aagggaacag
     2521 atgtccagcg tggatactgc gtggttgcgg atggaaagcc ccgtgtcgca tatgaccatt
     2581 g
//
LOCUS       DASUEG010000805         6492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_584383, whole genome shotgun sequence.
ACCESSION   DASUEG010000805 DASUEG010000000
VERSION     DASUEG010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584383"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1895
                     /locus_tag="VFX11_15150"
     CDS             183..1895
                     /locus_tag="VFX11_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940015.1"
                     /translation="MITIEGSASLNLVKEQIDESLSIAESNLEQFVEEPDNDSRLRAS
                     IEQFSQIKGVFKLINLPSAAMLAEELELLGLRILARHKQNNERELATISGSIMLLAHY
                     LEYVQVKKQALPVLLIPAVNEVRGLLGKPLVAESQFYDLADNPHRPDKPGSPAPDVAQ
                     LEQTGRRLRHMYQVGMLGVFRQQNIPTNVKLMQRALSRLDALCGNAPASKLLWLAQGV
                     LEGLGSGKLEVNTARKSLLGSIDRHIKPLVFQTEAALKKETPSTLVKECVYLAALAGP
                     AGPVLNEIFSVFHLDPSGVRDETLRAERDIMRGPGGSVIRSVAEALNEELTHIKDALD
                     LGARGASADAEGFNTTAEAMRKVANTLIMLGLSKPAGLFKEQSEIVRRWSTGNVDGDS
                     AEFNKVADALLYVENSIATLAPPRGPDSANYPEAAENVIKSGMSVTQLDDARRVVVAE
                     SRAGLSLAKRAITSFMDSNWDGMHLNNVPVTLKSVWGGLIFLQLPRAAEVVRACEDFI
                     EQRLIRGLSEKPSIQMMETLADAITGVDYFLEGMEDNKPIGEGILEVAEESMQELGFP
                     VKAA"
     gene            1926..2750
                     /gene="nudC"
                     /locus_tag="VFX11_15155"
     CDS             1926..2750
                     /gene="nudC"
                     /locus_tag="VFX11_15155"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX4940016.1"
                     /translation="MEFIAAVVPPSGIEGESIWFAFHEGQLLMFQDEQGSLIPQLARF
                     SDLGISHRKPHYLGTLAGQHCFAVTIEPGSDLPDQFYLQDLRSLAVEVDPALFMLAGR
                     ARQILEWDDTHRYCSRCGTPTQAHAYDRAKLCPTCGFTQYPRIAPCIIVLVTRGYEVL
                     LARSAHFPPGMFSTLAGFVEPGETLEMAVHREVEEEVGIKVTNLRYLASQPWPFPHSL
                     MIGFHADYAGGEIRVDGVEIEQADWWPVNSLPKTPPRGSISRFLIDQYLDQFALNR"
     gene            2767..3423
                     /locus_tag="VFX11_15160"
     CDS             2767..3423
                     /locus_tag="VFX11_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940017.1"
                     /translation="MHYQWITVVFALGSLTALIVALILLTRRNWFTGWLKGTGGLLAL
                     VAAIVLAITAINANGYRPLNEEYTVATIGFDRIDNQIFRATLVMQSSGTEMPFEIAGD
                     LWQIDARVIKWKGPLAAFGAQPGYKLDRLQGRYYSLEDEQTRARSIYALSNPDIGFDL
                     WSVLRGLSRHVDWFDAEYGSATYLPMADGAQFSVRLTASGMIARPENEQAEQAVRDWQ
                     "
     gene            3513..4565
                     /gene="sohB"
                     /locus_tag="VFX11_15165"
     CDS             3513..4565
                     /gene="sohB"
                     /locus_tag="VFX11_15165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEX4940018.1"
                     /translation="MVEFFTEYGLFLAKAVTVVVAILLIITAIAAAAQSRAHHGHKGE
                     IRVTPLNDFFEDLKLALQHNIFTKEEMKAEEKRLKKEEKQKEKERKKHPEQPDRKRIF
                     VLDFDGDMKASGVENLANEITAVLTLARKEDEVVLRLESPGGQVHAYGLAASQLERVR
                     QKGVPLTVCVDMVAASGGYMMACVADKIIAAPFAILGSIGVVAELPNFNRALKKFDVD
                     YDIYTAGQFKRTVTMLGENTEAGKQKFIEEIEDTHGLFKAMVSRNRPKLDMNRIATGE
                     HWYGNQCLENGLIDVLQTSDDYLYEASANADIFEVCYEIKRPLADRLGLAMEGVLSKS
                     VLRVMETLSGRGKQLL"
     gene            4604..5608
                     /locus_tag="VFX11_15170"
     CDS             4604..5608
                     /locus_tag="VFX11_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEX4940019.1"
                     /translation="MNGTFRALRVSETANGIEQAIVERSLDDLPAGDVLIRVSHSSVN
                     YKDALSASGNKGVTRQYPHTPGIDAAGVVESSSEPSIRVGDEVLVMGYDLGMNTSGGF
                     SQYVRVPASWVIRKPAGLSLENAMVFGTAGFTAAMCVDTLLQVGISPDQGPVLVTGAT
                     GGVGIVAVWLLHNQGFEVVAATGKPGQEGLLKAVGADSVISRASLLENASRPMNKPVW
                     AAAVDTVGGDILGNILKSIRYGGSVACCGMVGGHQFTTSVFPFILRGVNLLGVDSVEL
                     PLDVKQDIWNMLGDKWTFDDFADFKQRTVKLISLDELPAALAEVLQGTHSGRYVVKLG
                     "
     gene            complement(5611..6453)
                     /locus_tag="VFX11_15175"
     CDS             complement(5611..6453)
                     /locus_tag="VFX11_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940020.1"
                     /translation="MNGIMDYWALLNTLVIMFALGLSGAKSFAGAHWPLLRSTLGYNL
                     LIPALALIIIKTMGWFAPETLAAMAMCIAAAGGTSAGAFVTKVKGSPVLAAKLIVLLL
                     GVSLLAVTVFSQLGWVELGTLSLGGLTVYLLTITLLPLFIGRGFHRLHPVMAARWQPW
                     FDRLGSLLVVLLVIALAVRYGREILSGPAEPMIAAALLVFLFVLPPWLEPEPGCRRTL
                     VLVTLIRNLTLVLSILAVLPQSASLMPTVLAFGLFMYLTTAILVWYWRIHPYKEPQTE
                     SAQD"
BASE COUNT         1394 a         1825 c         1979 g         1294 t
ORIGIN      
        1 acagcacgtt agacgcacta tctataactg gcattagcgt ttggcctcag gtagccttca
       61 ccaaaggcct tatgtccttg aagtggaaca aaaaaaagaa tctatacttt ttgatgctca
      121 gcgctgaaag gacctgtccc ccgttgagcc tatctggcct gaacaaaaaa gacggtggtc
      181 tcatgataac aattgaaggt tccgcatccc tgaatctggt gaaggaacag atcgacgaat
      241 cgctctcaat tgccgaatcc aatctggagc agtttgttga agagccggac aatgattcgc
      301 gcctgcgggc ctccatcgag cagttttccc agatcaaggg tgtgttcaaa ctgatcaatc
      361 tgcccagtgc cgccatgctg gcagaggagc tggagttact ggggctgcgc attctggctc
      421 gacacaagca gaacaatgag cgggagctgg ccaccatcag cggttccatc atgctgctgg
      481 cgcactacct ggaatatgtg caggtaaaaa agcaggcgct gccggtactg ctgattcccg
      541 ccgtcaacga agtgcgggga ttgctgggca aaccgctggt agccgagagc cagttttacg
      601 atctggcaga caatccccat cgaccggaca agccgggcag tccggctccg gacgtcgcgc
      661 aactggaaca gaccggacgc cggctgcgcc acatgtacca ggtgggcatg ctgggtgtgt
      721 tccggcagca gaatattccc accaacgtca aactgatgca gcgtgcgctg agccgtctcg
      781 atgccctgtg cggcaatgcg ccggcgtcga agttgctgtg gctggcacag ggcgttttgg
      841 agggccttgg cagcggcaag ctggaagtga acaccgcgcg caaatccctg ctgggatcga
      901 tcgaccgtca catcaagccg ctggtgttcc agactgaagc cgcgctgaag aaggagacgc
      961 cttccacgct ggtgaaagag tgcgtctacc tggcggcgct ggctggccct gccggccccg
     1021 tactgaatga aatattttct gtcttccatc tggacccaag cggtgtgcgc gatgaaaccc
     1081 tgcgtgccga gcgggacatc atgcgtggcc ccggcggatc agtgatccgc tcggtggcgg
     1141 aagcgctgaa cgaggagctc acccacatca aggacgcgct ggacctgggt gcccgtggtg
     1201 cctctgcaga cgcagaaggt ttcaacacta ccgctgaagc catgcgcaag gtggccaata
     1261 ccctgatcat gctgggcctg agcaagcccg ccggcctgtt caaagagcag tccgagatcg
     1321 tcagacgctg gtccaccggc aatgtggatg gcgattccgc cgaattcaac aaagtggccg
     1381 atgcgctgtt gtacgtggaa aacagtatcg caaccctggc accaccgcgc ggaccggaca
     1441 gtgcgaatta cccggaagcg gcggagaacg tcatcaagtc cggcatgtcg gtcactcagc
     1501 tggatgacgc ccgccgcgtg gtggtggcgg aatcccgtgc cggcctgtcg ctggccaagc
     1561 gtgcgatcac gtcgttcatg gattccaact gggacggcat gcacctgaac aacgtgccgg
     1621 tgacgctgaa atccgtctgg ggtggcctga tcttcctgca attgccacgt gctgccgagg
     1681 tggtgcgggc ctgcgaggat ttcattgagc agcgcctgat tcgcggtctc agtgaaaagc
     1741 cgagcatcca gatgatggaa accctggcgg acgccattac cggcgtcgat tattttctgg
     1801 aaggcatgga agacaacaag cccattggcg aaggcattct ggaagtggcc gaggaaagca
     1861 tgcaggaact gggttttccg gtgaaagcgg cctgatcggc ggaatctgat ataacaggag
     1921 tggggatgga gttcattgca gccgtggttc cgccgtccgg gatcgaggga gagagtatct
     1981 ggttcgcctt ccatgaaggc cagctgctga tgtttcagga cgagcaggga tcactgatcc
     2041 cgcaactggc gcgtttttcc gatctgggaa tttcccatcg caagcctcac tatctgggaa
     2101 ccctggcagg ccagcactgt tttgccgtca ccatcgaacc gggcagcgat ctgccggacc
     2161 agttttacct gcaggatttg cgctcgctgg cagtggaggt ggacccggcc ctgttcatgc
     2221 tggccggccg cgcccgccag attctggagt gggacgacac ccaccgttac tgcagccgct
     2281 gcggcacacc cacccaggcc cacgcctacg accgcgccaa actctgtccc acctgcggct
     2341 tcacccagta tccgcggatt gccccctgta tcatcgtact ggtgacacgg ggctatgagg
     2401 tgttgctggc ccgttccgcc cattttccac cgggcatgtt ttccacactg gcgggatttg
     2461 tggagcccgg cgaaacgctg gaaatggccg tgcaccggga agtggaggag gaggtgggta
     2521 tcaaggtcac caacctgcgc tatctcgcga gccagccctg gccgttcccc cattcgctga
     2581 tgatcgggtt tcacgccgac tatgccggtg gcgagatccg ggtggatggc gttgagatcg
     2641 aacaggccga ctggtggccc gtgaacagtc tgcccaagac accgccgcgg ggctccattt
     2701 cccgttttct gattgaccag tatctggacc aatttgcgct gaaccgctag gactgccgga
     2761 aaccttatgc actatcaatg gatcactgtt gtattcgcac tgggcagcct gaccgcgctg
     2821 atcgtcgcgc tgattctgct gacccgccgc aactggttca ccggctggct gaagggtacc
     2881 ggtggtctgc tggcgctggt cgcggcgata gtcctggcca tcaccgcgat caacgccaac
     2941 ggctaccggc cgctgaacga ggagtatacg gtggccacca tcggctttga tcgaatcgac
     3001 aaccagattt tcagggccac tttggtaatg cagtccagcg gtactgaaat gccattcgag
     3061 atcgccggcg acctgtggca gatcgatgcc cgagtcatca agtggaaggg accgctggcg
     3121 gcgtttggcg cccagcccgg ctacaagctg gatcgattgc agggtcgcta ctattccctg
     3181 gaggatgaac agacccgggc tcgcagcatc tatgccctga gcaatcccga tattggtttc
     3241 gacctgtggt cggtgttgcg gggcctgtcg cgccatgtgg actggtttga cgccgagtac
     3301 ggaagcgcca cctacctgcc catggccgat ggcgcccagt tcagcgtgcg tctgaccgcc
     3361 agtggcatga tcgcccgccc tgaaaacgaa caggccgaac aggccgtgcg cgactggcaa
     3421 tagcggtagg attcaacgga cctttgcggt atctttaggc ccgtccgtgg ggccaatccc
     3481 cgccttgcac acatccccaa tgaggttttc gagtggtcga gtttttcact gaatatggcc
     3541 tgttcctcgc caaggccgtt actgttgtcg ttgccatcct gctgatcatc accgccatcg
     3601 ccgctgcagc ccagagccgg gcacaccatg gccacaaggg cgagatccgc gtcactcccc
     3661 tcaatgattt ctttgaagac ctgaagctgg cgctgcagca caatatcttc accaaggagg
     3721 aaatgaaggc ggaggaaaaa cgcctcaaga aggaagaaaa gcaaaaggaa aaggaacgca
     3781 agaagcatcc cgagcagccg gatcgcaagc gcattttcgt gctggatttc gatggtgaca
     3841 tgaaagccag tggcgtggaa aacctggcca atgaaatcac tgccgtgctg acgctggcgc
     3901 gcaaggaaga tgaggtggtg ctgcgcctgg aaagtcctgg cgggcaggtg catgcctatg
     3961 gcctggcggc atcgcagctg gaacgggtgc gacagaaagg tgtaccactg acagtgtgtg
     4021 tggacatggt ggccgccagc ggcggttaca tgatggcctg cgtggcggac aagatcatcg
     4081 ccgcgccttt cgccatactg ggttccatcg gcgtcgtggc ggaactgccc aatttcaatc
     4141 gggccctgaa gaaattcgat gtggattacg acatttacac ggccgggcag ttcaagcgca
     4201 ccgtcaccat gctgggcgag aacaccgaag caggcaaaca gaaattcatt gaggaaatcg
     4261 aagatactca cggcctgttc aaggcgatgg tgagccgcaa ccgtcccaag ctcgacatga
     4321 accggattgc caccggcgag cactggtacg gcaatcaatg tctggaaaac ggcctgatcg
     4381 atgtgctgca gaccagcgac gattatctgt atgaagccag cgccaacgcg gatattttcg
     4441 aagtctgcta cgagatcaag cgtccactgg ccgatcgtct tgggctggcg atggaagggg
     4501 tgctgagcaa aagtgtgctg cgggtaatgg aaacgctgtc cgggcgtggc aagcagttgc
     4561 tctgaaaccg gcgaccaatg attcaatact ttgagggaac gacatgaacg gcacattcag
     4621 ggcgctgcgg gtcagcgaaa ccgcaaacgg aattgagcag gcgattgtcg agcgcagtct
     4681 cgacgatttg ccggctggcg atgtgctgat ccgggtgagt cattcctcgg tgaattacaa
     4741 ggatgctctg tcggcctcgg gcaacaaggg ggtaacccgt cagtacccgc atacacccgg
     4801 cattgatgcg gccggcgtgg tggaaagttc aagtgagccg tcgatccggg tgggtgatga
     4861 agttctggtg atggggtacg acctgggcat gaataccagc ggtggcttca gtcagtacgt
     4921 gcgcgtgcct gccagctggg tgattcgcaa gcctgccggg ctgagcctgg aaaatgccat
     4981 ggtgtttggc acggccgggt tcacggctgc catgtgcgtg gatacgctgc tgcaggtggg
     5041 tatctcgccg gatcagggcc ccgtgctcgt aaccggcgcc accggtggcg tgggcattgt
     5101 cgcggtgtgg ctgctgcaca atcagggctt tgaagtcgtg gccgccaccg gcaagcccgg
     5161 gcaggaagga ttgctgaaag ccgttggggc ggacagtgtg atatcccgcg cgtcgttgct
     5221 ggaaaacgcc agtcgcccca tgaacaaacc tgtatgggcc gctgctgtgg atacggttgg
     5281 tggtgacatt ctgggcaaca ttctgaaatc gatacgttac ggcggcagtg tcgcctgctg
     5341 cggcatggtg ggggggcacc agttcaccac cagcgtgttt cccttcatcc tgcgcggtgt
     5401 aaatctgttg ggcgtggatt cggtggagtt acccctggat gtcaagcagg acatctggaa
     5461 catgttgggt gacaagtgga ccttcgacga tttcgccgac ttcaaacagc gtaccgtcaa
     5521 gctgatcagt ctggatgaac tgcccgctgc ccttgcggaa gtgctgcagg gcactcacag
     5581 tggccgttat gtggtgaagc tgggctgaat tcagtcctgc gccgattcag tctgcggttc
     5641 tttgtatggg tgaatgcgcc agtaccacac cagtatggcc gtcgtcaggt acatgaataa
     5701 tccaaaagcc agaaccgtcg gcatcagtga tgctgattgt ggcagcactg caagaatcga
     5761 cagcaccagc gtgaggttgc ggatcagggt aaccaacacc agcgtgcgac ggcagcccgg
     5821 ttccggttcc agccaggggg gcaatacaaa cagaaacacc agcagggcgg cggcaatcat
     5881 tggttccgcc ggcccgctca gaatttcacg tccgtaacgc accgccagcg caatcaccag
     5941 caatactacc agcaaacttc ccagccgatc gaaccagggt tgccagcgcg cggccatgac
     6001 cgggtgtaaa cgatgaaaac cgcgaccaat gaacagtggc aaaagcgtga tggtcagcag
     6061 gtaaacggta agccctccca acgacagcgt accgagttcg acccaaccca gttgcgaaaa
     6121 aaccgttacg gccagcaggc tgacacccag cagcagtacg atcagtttcg ccgccagtac
     6181 cggtgatccc ttcactttcg tcacaaaagc gcccgcggac gttcccccgg cagcggcaat
     6241 gcacatcgcc atggcggcca gtgtctcggg tgcgaaccag cccatggtct tgatgatgat
     6301 gagtgccagc gctggtatca ggagattgta cccaagcgtg gatcgcaaca gcggccagtg
     6361 cgcacctgca aaagattttg cacctgacag tcccagtgca aacatgatga ccagcgtgtt
     6421 cagcaacgcc cagtaatcca tgatcccgtt catttatttt cttcactcat ttttttgtgc
     6481 gttgaaatta tc
//
LOCUS       DASUEG010000806         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_76491, whole genome shotgun sequence.
ACCESSION   DASUEG010000806 DASUEG010000000
VERSION     DASUEG010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76491"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..640)
                     /locus_tag="VFX11_15180"
     CDS             complement(254..640)
                     /locus_tag="VFX11_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940021.1"
                     /translation="MRFWILLLILGWRMRWLAKHNEKFREKLENKDIVLQFRTAGGQV
                     ARYYVVQNQTVVPHAGIHPKPSMCMSFRDAKYAFETIMKAGKDQMVFMKGMGSKDIIV
                     TGEAAEMMWFMSLIKFLPPKKKKKNG"
     gene            complement(668..940)
                     /locus_tag="VFX11_15185"
     CDS             complement(668..940)
                     /locus_tag="VFX11_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016915488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX4940022.1"
                     /translation="MQKTEVEAALAEQVGISKTAARDLLNVILDEVTLALSRGQRIAL
                     PGFGVFEVRAHGPRKGRNPQTGEPLEIPAGHAVHFKPGKALKASIV"
     gene            complement(1039..2427)
                     /gene="dbpA"
                     /locus_tag="VFX11_15190"
     CDS             complement(1039..2427)
                     /gene="dbpA"
                     /locus_tag="VFX11_15190"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEX4940023.1"
                     /translation="MSQTDFASLGLSAELLTNLASMGYAEMTPIQAQSLPPILAGKDV
                     IAQGKTGSGKTAAFGLGLLAKLDVKRFCIQVLVLCPTRELADQVAKEIRKLARTTHNL
                     KVLTLCGGMPFGPQLGSLEHGAHIIVGTPGRVEEHLRKGSLVLDNLHTLVLDEADRML
                     EMGFQAALDAIIEKAPKQRQTLLFSATFPDQIQRVAKRIMQQPVMVKVESTHDSASIV
                     QHFYKVENDEQRLPALRLLLLQFAPESAVVFCNTKKETQEIADALHKDGFSVLALHGD
                     LEQRDRDETLVRFANKSACILVATDVAARGLDIDNIEAVINYQLAHDSEVHVHRIGRS
                     GRAGSKGFACSLFTDKDHYKVALIQDYLGQIITGEPLPPRSVLNNSPLQASMATLQID
                     SGKKQKIRPGDILGALTGENGIPGAQVGKIHLFDNWAYVAVSRDAVKPALKKLAEDKL
                     KGRSVKVRWIRD"
BASE COUNT          489 a          760 c          725 g          544 t
ORIGIN      
        1 cagactcagc ggcagatact gccgacggca cgaaaataag cggtttccct gttgttcccg
       61 gttggcgtcc ctcacccctg tccccacaac ttgttcaaaa tacattcaaa cttgcctggg
      121 tgccagctta gcaaccagcc agcaccggcg ccactgcggg cagaagtttc gtcacacaaa
      181 cgctacaaaa caagtaacta ttcccggttt tgtaacctga gaccgaatga aatgaaccgt
      241 tgtgaaaagg atctcagccg tttttcttct ttttcttcgg cggcaggaac ttgatcaggc
      301 tcatgaacca catcatctcg gctgcttcac cggtgacgat gatgtccttg ctgcccatgc
      361 ccttcatgaa caccatctgg tccttgccgg ccttcatgat ggtttcgaag gcatatttgg
      421 catcgcggaa gctcatgcac atgctgggct tgggatggat gccggcgtgg ggcaccacgg
      481 tctggttctg caccacgtaa tagcgggcca cctggccacc ggcggtgcgg aactgcagca
      541 caatgtcctt gttttccagc ttttcacgaa acttttcgtt gtgcttcgcc agccagcgca
      601 tgcgccagcc gaggatcaac agcaaaatcc agaacctcat aacaccattc ccgttaacag
      661 tagaaaatca aacaatgctc gccttcagcg ccttgcccgg cttgaagtgg acggcatggc
      721 cggccggaat ctccagcggc tcgccggttt gcgggttgcg tcctttccgg ggcccgtggg
      781 cccgcacttc gaacacgcca aagccgggta atgcaatccg ctggccacgg ctgagggcca
      841 gggtgacctc gtccagaatc acgttgagca ggtcgcgggc ggccgtcttg ctgatgccca
      901 cttgctccgc cagcgcggct tccacttcgg ttttttgcat gggatcggcc cggtagcgag
      961 aaagggggcg attgtatacc ggttgcgggc aagggggaat ccgaaggaat cgatagccct
     1021 ataaaacggg tgaaccgctc aatcccgaat ccagcgcacc ttcaccgacc gccccttcag
     1081 cttgtcctcg gccagttttt tcagggcagg tttgacggcg tcgcgactca ccgccacata
     1141 agcccagttg tcgaacaggt gtattttgcc cacctgggcg ccgggaatac cgttctcgcc
     1201 ggtgagcgcg ccgagaatat cgccggggcg gattttctgt ttcttgccac tgtcgatctg
     1261 caacgtggcc atggatgcct gcagcgggga attgtttagc acactgcgcg gcggcagcgg
     1321 ttcgccggtg atgatctggc ccagataatc ctggatcagc gccactttgt aatgatcctt
     1381 gtcggtgaac agcgagcagg cgaaaccctt gctgccggca cgtccgctgc gaccgatgcg
     1441 gtgcacgtgc acttccgaat cgtgcgccag ctggtagttg atcacggctt cgatgttgtc
     1501 gatatccagc ccccgcgccg ccacatcggt cgccaccaga atgcaggcgc ttttgttggc
     1561 gaaccgcacc agggtttcgt cgcggtcgcg ctgctccaga tcaccgtgca gcgccagcac
     1621 gctgaaacca tccttgtgca gcgcgtcggc aatttcctgg gtttcctttt tggtgttgca
     1681 gaacaccacg gcggattccg gtgcgaactg cagcagcaac aagcgcaagg ccggcagacg
     1741 ctgctcatcg ttttccacct tgtagaaatg ctggacgatg ctggcgctgt cgtgggtgga
     1801 ttccactttc accatgaccg gctgctgcat gatgcgtttg gcgacacgct ggatctggtc
     1861 gggaaaggtg gcactgaaca gcagggtctg gcgctgtttc ggtgcttttt cgatgatggc
     1921 gtccagcgct gcctgaaaac ccatttccag catgcggtcc gcttcgtcca aaacaagcgt
     1981 gtgcagattg tcgagcacca gactgccctt gcgcagatgc tcctccaccc gccccggcgt
     2041 gccgacgatg atgtgcgcgc cgtgctccag cgaacccagt tgcggaccga acggcatgcc
     2101 gccgcacagg gtcagcactt tcaggttgtg ggtggtgcgc gccagcttgc ggatctcctt
     2161 cgccacctga tccgccagtt cacgggtggg acacagcacc agcacctgta tgcaaaaccg
     2221 tttgacgtcg agtttcgcca gcaggcccaa tccgaacgcg gcggttttgc ccgagccggt
     2281 cttgccctgg gcaatcacgt ccttgcccgc caggatcggc ggcaggctct gggcctggat
     2341 cggtgtcatt tcggcatagc ccatcgacgc caggttggtg agcagttcgg cggacaatcc
     2401 aagggaagca aaatcggtct ggctcacggt gcaggtcctg taaaaaaaga gcggtgtaaa
     2461 aacgaaaatc cccgtcacct tgcgatgacg gggacttcgc attgacggca gtggctga
//
LOCUS       DASUEG010000807         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_656982, whole genome shotgun sequence.
ACCESSION   DASUEG010000807 DASUEG010000000
VERSION     DASUEG010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656982"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="gcvPA"
                     /locus_tag="VFX11_15195"
     CDS             <1..998
                     /gene="gcvPA"
                     /locus_tag="VFX11_15195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX4940024.1"
                     /translation="AMDVSNASLYDGGSGLSEAVLMAVRANRKSKSKKALLLQTVNPL
                     YRSATRNIVHNQGIELVEVPYDPTTGVTALDAMKKFDGQDFAAIVIQQPNFFGSLEDV
                     HALTNWAHANNLLVIASVNPTSLAILTPPGEWGDSGADIATGEGQPLGAPLSSGGPYF
                     GFITCKMEHVRQMPGRIIGRTVDLEGRPGFTLTLQAREQHIRRSKATSNICTNQGLAM
                     TAATIYMSLLGPDGLQRVAENSHTNTLALVEKLTAIAGVKKVFSAPNFHEAVIQVPHK
                     ASDVLQKLAAQKIQGGVDLGAFYPGLENCILLCATETKTAADLDKYAAALQSVLK"
     gene            1009..>2131
                     /gene="gcvPB"
                     /locus_tag="VFX11_15200"
     CDS             1009..>2131
                     /gene="gcvPB"
                     /locus_tag="VFX11_15200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX4940025.1"
                     /translation="MLIFEKSQSGRRATSQAPMQSSAASDIPQNLRRKQKPLLPEVGE
                     MQVVRHYTNLSRKNFSIDTMFYPLGSCTMKYNPRGAHRGAMLPGFLGRHPLAPEQHSQ
                     GFLACLHDLQEVLKDVTGMKGVSLTPMAGAQGEFAGVAMIRAYHEARNDHERTEILIP
                     NAAHGTNPATAVMCGYKVREIPVNDEGDVDVDALKAAVGPHTAGLMMTNPSTCGVFER
                     NIKTIAQVVHQAGGLLYYDGANLNAILGKVRPGDMGFDVLHMNLHKTFATPHGGGGPG
                     AGPVGVGERLLPYLPTPIVGKEKVNGVDTYRWLTDKDIPGTIGNLSTYMGNAGVLLRA
                     FFYASTLGREGMHRVGEYSSLNANYLMAKLAKAGFEMAYP"
BASE COUNT          447 a          658 c          651 g          375 t
ORIGIN      
        1 tggcgatgga tgtgtcgaat gcgtcgctgt atgacggtgg ctcgggtctg tcggaagcgg
       61 tattgatggc ggtgcgcgcc aaccgcaaat ccaaatcgaa aaaagcgctg ctgctgcaga
      121 cggtgaatcc gctttaccgc agcgccacgc gcaatatcgt gcacaaccag ggcatcgaac
      181 tggtggaagt gccgtacgat ccgaccaccg gtgtgacggc gctggatgcg atgaagaaat
      241 tcgacggcca ggattttgcc gcgatcgtga tccagcaacc caacttcttc ggctcgctgg
      301 aagacgtgca cgcgctcacc aactgggcgc acgccaacaa tctgctggtg attgcgtcgg
      361 tgaatccgac ttcacttgcc attctcacgc cgcccggcga atggggcgac agtggcgccg
      421 atatcgcgac cggcgaaggc cagccgctgg gcgcgccgct gtcgtcaggc ggcccgtact
      481 tcggtttcat cacctgcaag atggaacacg tgcgccagat gccgggccgc attatcggtc
      541 gcaccgtgga tctggaaggc cgccccggtt ttacgctgac actgcaggcg cgggaacagc
      601 acatccgccg ctcgaaagcg acatcgaaca tctgcaccaa ccagggcctg gcgatgaccg
      661 ctgccaccat ttatatgagt ttgctcggcc ccgatggcct gcagcgcgtg gcggaaaatt
      721 cccacaccaa cacgctggcg ctggtggaaa agctcaccgc cattgccggc gtgaagaaag
      781 tgttcagcgc accgaatttc cacgaagccg tgattcaggt tccgcacaaa gcctctgacg
      841 tgctgcaaaa actggcagcg cagaaaatcc agggcggcgt tgatctgggc gcgttctatc
      901 ccggcctgga aaactgcatt ctgctgtgcg cgaccgaaac caaaacggct gcggatctgg
      961 ataaatacgc cgccgccctg caatccgtat tgaagtgagg agcctgccat gctgattttc
     1021 gaaaagagcc aaagtggacg ccgtgccacc tcgcaggcgc cgatgcaatc cagcgccgcc
     1081 agtgacattc cgcagaatct gcgccgcaaa caaaagccgt tgttgccgga agtgggcgag
     1141 atgcaggtgg tgcgtcacta caccaacctg tcgcgcaaaa acttcagcat cgacaccatg
     1201 ttctacccgc tgggcagctg caccatgaaa tacaacccgc gcggcgcaca ccgtggcgcc
     1261 atgctgccgg gttttctggg ccgtcacccg ctggcgccgg agcagcacag ccagggtttt
     1321 ctggcctgtc tgcatgacct gcaggaagtg ctgaaagatg tcaccggcat gaaaggcgtg
     1381 tcgctgacgc cgatggcggg cgcgcagggc gaattcgccg gcgtcgccat gattcgcgcc
     1441 tatcacgaag cgcgcaacga ccatgaacgt accgagattc tgattccgaa cgctgcgcac
     1501 ggcaccaatc ccgctaccgc cgtgatgtgc ggctacaagg tgcgggaaat tccggtgaac
     1561 gacgaaggcg acgtggatgt tgatgcgctg aaagcggcgg tcggcccgca cactgcagga
     1621 ttgatgatga cgaatccgtc cacctgcggc gtgttcgagc gcaacatcaa aaccatcgca
     1681 caggtggtgc accaggccgg cggcctgctg tattacgacg gcgccaacct gaacgcgatt
     1741 ctgggcaagg tgcgccccgg cgacatgggc ttcgacgtgc tgcacatgaa cctgcacaaa
     1801 acctttgcca cgccccacgg cggtggtggc cccggtgccg gcccggtggg cgtgggcgag
     1861 cgtctgctgc cgtatctgcc gacgccgatc gtgggcaagg aaaaagtgaa cggcgtggac
     1921 acctatcgct ggctcaccga caaggatatt cccggcacca tcggcaacct gtccacctac
     1981 atgggcaatg ccggcgttct gctgcgcgca tttttctacg ccagcacgct gggtcgcgaa
     2041 ggcatgcatc gcgtcggcga gtattcctcg ctcaacgcga actacctgat ggcgaagctg
     2101 gcgaaggccg gcttcgagat ggcctacccg a
//
LOCUS       DASUEG010000808         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1313509, whole genome shotgun sequence.
ACCESSION   DASUEG010000808 DASUEG010000000
VERSION     DASUEG010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313509"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFX11_15205"
     CDS             <1..266
                     /locus_tag="VFX11_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940026.1"
                     /translation="SRTLEINPIDFSVVWEFNHPRHGLIMGNVQRLPNGNTFISEGLT
                     SVMLEVTPEKEVVWQARGLLPQYRAYRVPKDWVTPTQNTAPAP"
     gene            416..697
                     /locus_tag="VFX11_15210"
     CDS             416..697
                     /locus_tag="VFX11_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940027.1"
                     /translation="MKLTRFLNALHIRGAALSLVLPVLLMTAVCNDKFEVSIYKPDKI
                     CESDTPVTYSAIGKTAALDLADKIIDEHPCTDNPVKLLRGSTTTSISAK"
     gene            complement(978..2114)
                     /locus_tag="VFX11_15215"
     CDS             complement(978..2114)
                     /locus_tag="VFX11_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4940028.1"
                     /translation="MLQRMFKAYGIVASQFNQRLSPLVTFTGFLLLHGLVTVGLAIDH
                     VFYPALRRTKIKSPIVIVGNPRSGTTFLHRFLVANDLGAGMRIWKMLYPSPTLQMLLK
                     PLLPLLEKISPARHHAHAAHETNLTAIETDDPSLLFRYFDGLFLYGFFYAWSKTETLD
                     EFERRFLANAERDFNWYETLWRRNLVSEKRSRVVAKLFSLSLRLPQFLRRFPDANVIY
                     MVRDPLETVPSGLSLVTGVLDGRFGFWSLPAEQRQHFIENLYAALLRLSLRFYEDYTA
                     GRIPSRNLYIVTFPRLMQQFDAVMEDILCFIDVDLTVEMQQQIRQIAEKQRTYRSGHE
                     YDLARFGLTEERIRRDYAVIYDTFLQNAPTSVQKHTNARLPYPA"
     gene            complement(2150..>2708)
                     /locus_tag="VFX11_15220"
     CDS             complement(2150..>2708)
                     /locus_tag="VFX11_15220"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX4940029.1"
                     /translation="EAGTPWRNLEQPESAALFGDAAAAALIEQSRGESAVQDWIMYTW
                     PEGSELTEFRGCGMRHPPGHPDTSSDDNLFNMQGPKVFRMVLMHISRVLRTLFERNGL
                     TYEDIDLFVLHQASGPGMEAFVKLGFPREKIVNVVADYGNCVAASIPMTLAIANEQGR
                     LRRGDRVLIGGTGAGLSIAFALLQW"
BASE COUNT          691 a          748 c          697 g          572 t
ORIGIN      
        1 gctcccgaac gctggaaatc aatcccatcg atttttccgt ggtgtgggaa ttcaatcacc
       61 cgcgacatgg cctgatcatg gggaatgtcc agcgtctgcc caatggtaac accttcatca
      121 gcgagggcct taccagcgtg atgctggaag tgacacccga aaaggaagtc gtgtggcaag
      181 cccggggctt gttgccgcaa taccgcgcct atcgggtacc gaaagactgg gtgacaccga
      241 cgcagaatac cgctcctgcg ccgtaattga ccctacagaa aatggcctgg tcctgtttgc
      301 acagcgacca ggccaatttt agacatccct tgcaccaatc gaaccgatca acccgatcga
      361 ttgcataaca ataacaagat gcagaaaaca ataatcaaaa aagggagcta cctccatgaa
      421 actgacgcgc tttttgaacg cgctgcatat ccgtggcgcc gcactgtcat tggtcttgcc
      481 agttctgctg atgacggcag tctgcaatga caaattcgaa gtttcgatct acaaacctga
      541 caagatctgt gagagcgaca ccccggtaac ctattcagcc attggtaaga cggcagcgtt
      601 agacctggca gacaaaatca ttgacgaaca cccctgcaca gacaatcccg tcaaactgtt
      661 gcgcggcagc accacgactt ccatttcagc gaagtgagca ggatattggc cggatttgcc
      721 agaagagggc aaacggcgga tattcgacga tggcaccact tcgggatatg cattatcaac
      781 gtgcgtctgg ccactgttcc tgcagatcgg aaatcagccc cccattgatt tcagcatgac
      841 gtggaagttc agtctggaca ccttgatttc agaaggcgcg agcggtcgca tgctgggagt
      901 gacgccgaaa aatgaagtgg cgtgggcagc tcgcggattg cagccctgca gtgcctgccg
      961 gctgtccatg aactgcatca agccggatat ggcagtcgag cgttcgtgtg cttctgcacc
     1021 gatgttggtg cattctgcag gaacgtgtcg tatatcacgg catagtcccg tcgaatgcgt
     1081 tcttctgtaa ggccaaagcg ggccagatca tactcgtgcc cgcttctgta agtgcgctgc
     1141 ttttctgcaa tctgccgaat ctgctgttgc atctcaacag tgagatcgac atcgatgaag
     1201 caaaggatat cttccattac cgcatcgaac tgctgcatca ggcggggaaa agtgacgata
     1261 tagagattgc gggagggaat ccggcctgca gtgtagtctt catagaaacg taaactcagg
     1321 cgcaacaacg cagcatacag attttcgatg aaatgctgac gctgttctgc cggcaacgac
     1381 cagaatccaa aacgaccgtc gagcacgcct gtcaccaggc tcagaccgga aggcaccgtt
     1441 tccagcgggt cacgcaccat atatataacg ttggcatctg gaaaacgtcg cagaaattgt
     1501 ggcaggcgca aggacaggga aaacagctta gccaccaccc ggctacgctt ttcgctcacc
     1561 agattgcgac gccacagcgt ttcataccag ttgaaatcac gctcagcgtt ggccaaaaag
     1621 cgacgttcaa attcgtccag tgtttctgtt ttcgaccagg cgtagaaaaa gccatacagg
     1681 aataatccgt caaaatagcg aaacagcagc gaagggtcgt ccgtttcgat cgctgtaaga
     1741 ttggtttcgt gtgcagcatg ggcgtgatgg cgggccggac tgatcttctc cagcagcggc
     1801 aatagcggtt tcagaagcat ttgcaatgtc ggtgacgggt acagcatttt ccagatgcgc
     1861 atacccgcgc ccagatcgtt cgccaccaga aagcggtgca gaaacgttgt gccgctacgg
     1921 gggttaccca cgataacaat gggactcttg attttggtac gacgcaaagc cggataaaac
     1981 acatgatcaa tcgccagccc cactgtcacc aatccatgca gtaacagaaa tccagtgaat
     2041 gtaaccaggg gcgatagacg ctgattgaat tggcttgcga caatgccata agccttgaac
     2101 atacgctgta acatgaatcc acttcccggc aagatttctt aagatggaat taccactgca
     2161 acagggcgaa ggcaatggac aaaccagccc cagtgccgcc aatcaatacc cgatcaccgc
     2221 gccgcaatcg accctgctca ttggcgatag cgagggtcat cggaatcgaa gctgccacgc
     2281 agttgccgta atcggcgacg acattgacga ttttttcccg cggaaatccc agtttcacga
     2341 atgcctccat cccggggccg gaagcctgat gcagcacaaa cagatcgata tcttcgtacg
     2401 tcaatccatt gcgttcgaac aatgtgcgca gcacccggga aatatgcatc agcaccattc
     2461 gaaacacctt ggggccctgc atattgaaaa ggttgtcatc actgctggta tccggatgcc
     2521 caggcggatg acgcatgccg caacccctga attcggtcag ctcggaccct tccggccagg
     2581 tgtacatgat ccagtcctgt acggcacttt cacctctgct ttgctcgatc agtgccgccg
     2641 cggccgcatc gccaaacaat gcagcgcttt ccggttgttc cagattgcgc cagggcgtac
     2701 cagcctcg
//
LOCUS       DASUEG010000809         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1562383, whole genome shotgun sequence.
ACCESSION   DASUEG010000809 DASUEG010000000
VERSION     DASUEG010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562383"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..964)
                     /locus_tag="VFX11_15225"
     CDS             complement(401..964)
                     /locus_tag="VFX11_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4940030.1"
                     /translation="MASQTKNALAFRRAARERVPIEVENYYVPFDRWYLIRFFPMNGE
                     GMVIIVQDMTERRRAEEALRESETRFRALAEASPGLIWQADPKGNAVYLNPRFRELLG
                     VPLKDLMGTAWHKLVHPDDLPSYVAALDKAIRQHGRLRHRVRVKRMDGEWRWLETNAL
                     PWFISEGEYAGHVGISIDITEALETAG"
     gene            complement(948..1514)
                     /locus_tag="VFX11_15230"
     CDS             complement(948..1514)
                     /locus_tag="VFX11_15230"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX4940031.1"
                     /translation="MLARQNWAMEQPRLVLRHLVFLDETWASTNMTPTRGRSLKGTRC
                     IGRAPYGHWRTTTFICALRSEGLIAPFVLDGPINGQSFRTWLQQVLIPALQPGDMVVL
                     DNLGAHKVTGIAAAIEAAGAQLRYLPPYSPDYNPIEQVFAKLKTALRRTATRTVDALW
                     STIGSLLDRFPAAECERYIRHAGYGQSD"
     gene            complement(1514..1903)
                     /locus_tag="VFX11_15235"
     CDS             complement(1514..1903)
                     /locus_tag="VFX11_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX4940032.1"
                     /translation="MMAWHQGQAYGQDLRDRVLGADGSIRAIAARFGVSKSYVARARS
                     RWRCLGDTAPGQQCNHVPAKLGGLEQALADRVAASNDQTLEQLCQWVHAEHGIRVSTA
                     TMYKTLARLGLRLKKNHFTPPSRRARM"
BASE COUNT          424 a          611 c          595 g          436 t
ORIGIN      
        1 aatagttcga ttttccgtgt gctaaagcgg cgagtacgac atggggagaa aaaagaggat
       61 gcgtcacggg atatagtggc cgaatgccca ccggtttcgc gttttcacac acgctcaacc
      121 aatagcagac gattacacgt aagaccagtt acgactctgt agacatcagc ttctgagtgc
      181 ctgccatggg cttcgggcat ctgaacaagt gtgccgcgac taccattgcc ggcatcgagt
      241 tgatgcatcg cattcgtaaa tggcagtttt tgctccacca gctgggcgtt caggggcaag
      301 ctgcgcccgc ggtgtgaaat gcggtactgg ctgtatgagc aggactgcat tccacttcag
      361 ttttgtaccc acatcgaaaa tttacaccag agccatccgg ttaccctgca gtctcgagcg
      421 cttcggtgat atcgatggag atgccgacgt gaccggcata ttccccctca gagatgaacc
      481 agggtagcgc attggtttcc agccagcgcc attctccatc cattcttttg acgcgcaccc
      541 gatgccgcag gcgcccgtgc tggcggatcg ccttgtcgag tgcggccaca taggatggca
      601 ggtcatcggg atgaaccaac ttatgccacg cggtgcccat gagatccttt aacggcacgc
      661 ctagcaattc ccggaaacga ggattcagat acactgcgtt tcccttggga tccgcctgcc
      721 agatcaaacc gggggaagcc tcggccagcg cacggaaccg ggtttcgctc tcacgcaagg
      781 cttcctcggc gcgcctgcgc tcggtcatgt cttgcacaat gatcaccatc ccttctccat
      841 tcatggggaa aaagcggatc agataccagc ggtcgaaagg tacatagtaa ttttcaactt
      901 cgatcggcac ccgctcacgc gccgcgcgcc gaaaggctag agcgttttta gtctgactgg
      961 ccatagccgg catgccggat gtatcgttca cattctgcgg cagggaaacg atcgagcaag
     1021 gagccgatag tcgaccagag ggcatcgacg gtacgggtgg cggttctcct gagtgcggtc
     1081 ttgagtttgg caaacacttg ctcgatgggg ttgtagtccg ggctgtacgg tggcaaatag
     1141 cgcaattgcg caccggccgc ctcgatggcg gcggcaatgc cagtcacctt atgcgccccc
     1201 agattgtcca gcaccaccat gtcaccgggt tgcagggctg ggatcaggac ctgttgcagc
     1261 caagtccgga aggactggcc attgatggga ccatccagca cgaagggagc aatcaggcct
     1321 tcgctgcgca gggcacagat aaaggtggtc gtgcgccagt ggccataagg agcacggcca
     1381 atacagcgag tgcctttcaa ggagcgcccg cgggtgggcg tcatattggt actggcccag
     1441 gtctcgtcca gaaacaccag atgccgcaac accagacgcg gctgctccat ggcccagttc
     1501 tggcgcgcta gcactacatc cgggcgcgtc tgctcggcgg cgtgaagtga ttttttttga
     1561 ggcgtagccc cagccgggcc agggtcttgt acatggtcgc tgtgctgaca cggatgccat
     1621 gttctgcatg tacccattgg cacaattgct ccagcgtttg gtcgttactg gcggccacgc
     1681 gatctgccag cgcttgttcc agtccgccca gctttgccgg cacatgattg cactgctgtc
     1741 ccggggccgt atcgcccagg cagcgccacc gggaacgggc gcgtgccacg taggacttgc
     1801 tcacgccaaa ccgggcggcg atcgctcgaa tggaaccgtc ggctcccaac acgcgatcgc
     1861 gcaaatcttg cccgtaggct tgtccttgat gccaggccat catcactctc ctgttactgc
     1921 ataggcgtct gatcctctcg tcacttgcca gttcagcaaa tccatggcca cagttaggct
     1981 aaaagcgctc tagtccaccg gcggactgcg ataacagctg ccgctagttg gcgaaccgtc
     2041 ctcgctgcga gttcactgtc attcag
//
LOCUS       DASUEG010000810         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1667146, whole genome shotgun sequence.
ACCESSION   DASUEG010000810 DASUEG010000000
VERSION     DASUEG010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667146"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFX11_15240"
     CDS             complement(<1..607)
                     /locus_tag="VFX11_15240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940033.1"
                     /translation="MEFNLPENTTLIADTARRYLKDNYSFEIYRQQLEQQNHVDASRW
                     SAMQELGWFGLPFAESSGGFDGTLLDVAMVVRELGAALCLDPYVDTVVAPGKLLEYIG
                     SDATLAMLSDVIDGRARLACALYDYHAGYQVLRPSMKLNGKRLSGQKNFVPDGGVADT
                     LLTTALADGELLVLAVPVAACETTRYRALDGGRIANVLFDEV"
     gene            complement(617..1777)
                     /locus_tag="VFX11_15245"
     CDS             complement(617..1777)
                     /locus_tag="VFX11_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940034.1"
                     /translation="MNLEFTQEELEFQQSIRDWMQANVPDDIKRCSAVGEMTGKELQQ
                     KWERLLGERGWLALTWPKQYGGPGWTATQRYIFDLERAMAGAPPTSPFGVAMVGPVLY
                     TFGSQEQKDRWLPGIVSGRTLWCQGYSEPNAGSDLASLKTRAERRDDHYLVNGQKIWT
                     TQAHWADMMFCLVRTDSTVKQQKGISFLLIDMKTPGIEVRPIYSIDGHHHLNEVYFTD
                     VRVPLENLVGQEGMGWTIAKFLLTHERTTIAGVADSHHEISRLKKAVAEAPGFLDKSQ
                     ARQRIAELEIELMALEYTNFRTVAATDEGKPFGPESSGLKIKGTELQQKVSQAMVELG
                     GMMSLTWDNPETVGSDIFNRATRRYNFLRACTIYGGSNEIQKNVLAKMLLGL"
     gene            complement(1825..>2038)
                     /locus_tag="VFX11_15250"
     CDS             complement(1825..>2038)
                     /locus_tag="VFX11_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HEX4940035.1"
                     /translation="EMVHAIVVLKEGHGLNEGELLAHCRALIAGYKLPRSIEFRSEPL
                     PVSGAGKILKNELRKPYWENKDRGVN"
BASE COUNT          448 a          635 c          501 g          454 t
ORIGIN      
        1 cgacctcatc aaacagcacg ttagcaatac ggccaccatc cagcgcccga taacgagtcg
       61 tctcgcaagc agcaacaggc acagcaagca ccagcagttc gccatcagca agcgcggttg
      121 tcagcaaggt atcggcaaca ccgccatccg gaacaaagtt tttctgaccg gacaggcgtt
      181 tgccattaag cttcatggaa ggccttaata cctgataacc ggcatgataa tcatacagcg
      241 cacaggccag cctggccctg ccgtcaataa cgtcgctcaa catggccagg gtcgcatcgg
      301 agccgatata ttccagcaac ttgcccggcg ctaccaccgt atcaacgtag ggatccagac
      361 ataacgcggc gcccagctcc ctgacaacca tcgccacgtc aaggagcgtg ccatcgaagc
      421 cgccgctgga ttcggcaaat ggcagaccaa accagcccag ctcctgcatg gccgaccagc
      481 gtgacgcgtc aacatggttc tgttgctcaa gctgctgcct gtagatttca aagctgtagt
      541 tatctttaag ataccggcga gcggtatccg cgataagggt ggtgttttcc gggaggttaa
      601 attccatcgt ctcgcctcaa agaccaagca gcatttttgc cagcacattc ttctggatct
      661 cgttggatcc accgtaaatg gtgcaggccc tcagaaagtt gtaacgtcgg gtggcgcgat
      721 tgaaaatatc gctgccaacc gtttccgggt tatcccaggt taacgacatc atgccgccca
      781 gctccaccat ggcctgtgat actttctgtt gaagctcggt gcccttgatt ttcaacccgg
      841 aggattccgg cccgaagggc ttgccctcat ccgtcgcggc aacagtacgg aagttcgtgt
      901 attccagcgc cattaactcg atttcaagtt cagcaatacg ttgccgcgcc tggcttttat
      961 ccagaaagcc cggcgcttcg gccacagctt tcttcagccg tgatatctcg tgatgactgt
     1021 ccgccacccc agcgatggtg gtgcgctcat gcgtcagcag gaacttggca atagtccagc
     1081 ccatgccttc ctggcccaca agattttcca gcggcacccg cacatcggtg aaataaacct
     1141 cgttaaggtg atgatggcca tcaatcgagt agatcggccg gacctcgata ccgggggtct
     1201 tcatatcgat caacaggaaa gaaatccctt tctgctgttt gaccgtcgag tcggttctga
     1261 ccagacagaa catcatgtcc gcccagtggg cctgggtcgt ccagatcttc tggccattca
     1321 ccagataatg gtcgtccctg cgctcggcac gcgtcttgag tgacgcaaga tccgatccgg
     1381 cattgggttc ggagtaaccc tgacaccaga gcgttctacc gctgacgata cccggcaacc
     1441 agcgatcctt ttgctcctgc gagccaaagg tataaagtac cggccctacc atcgccaccc
     1501 cgaacgggct cgtgggaggc gcgcccgcca tggctcgctc aagatcgaaa atataacgtt
     1561 gcgtagccgt ccagcccggg ccgccatact gtttcggcca cgtcagtgcc agccagcccc
     1621 gctcaccgag caaacgctcc cacttttgct gcagttcctt gccggtcatc tcaccaaccg
     1681 ccgagcagcg cttgatatca tccggcacat tcgcctgcat ccagtcacga atggattgct
     1741 gaaactccag ttcttcctga gtgaactcca gattcacggc ttattacctc ttgcaggcca
     1801 tcggttgctg ttttcaattg tcattcagtt gactccccgg tctttgtttt cccaataagg
     1861 ctttctcagt tcgttcttga gaatcttgcc cgcaccactt accggtagtg gctcgctccg
     1921 aaactcgatg ctgcgcggca gcttgtaccc ggcaatcagt gcgcggcaat gggccaaaag
     1981 ctcgccttca ttgaggccat gcccttcctt gagcacgaca atcgcatgaa ccatctcg
//
LOCUS       DASUEG010000811         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1360189, whole genome shotgun sequence.
ACCESSION   DASUEG010000811 DASUEG010000000
VERSION     DASUEG010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360189"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VFX11_15255"
     CDS             <1..1658
                     /locus_tag="VFX11_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX4940036.1"
                     /translation="TVIMLASIIKQFHFKRLQRTTHNPAPRQQQILLDILRTNRNTEF
                     GQTHQFSAIQSIEDFRRQVPLRNSAEFGPYLKRVHQGERNVLTVETPYFFAMTAGSTG
                     DYKYIPITRRFKRELDRSVYAFYHLLETNCPEFRSAPLQFFVGSAEGGQSPGGIPQGF
                     VSGFNYKNLPAFIRRKFVVPYWVFTQPDMDDRFYAIGRFLTAKNSLAGFGGFSPLAIM
                     SVIKRLLANIDTLEQDVRNGTLTLNHPITPNRYTPTSLPTFKPDAALAQKISDWRNTE
                     RPLPDLMQILLPNLKYVATWMGGNMGYSTQSLLQLIGTKQVHEMPFSASEGIFGIPFR
                     LDREGGIAAVTSHFLEYIREQDIDLENPPVYCAWELEQGEHYYQIITTSGGLYRYNME
                     DLIRVEGFYNKTPVVQFVSKRARQISISNERLNENDVTESCRQASASTGVHFDEFILF
                     PTRANHYCLVVEECDQDLGPFVQAFEARLRALAKVYDMERTARTLGPVTLLETHRGQL
                     RDYTNAIYFRSALPSSQYKPVHLSNSFTDADSFPVARLHSLEI"
     gene            1799..2242
                     /locus_tag="VFX11_15260"
     CDS             1799..2242
                     /locus_tag="VFX11_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4940037.1"
                     /translation="MSSLRPYNSPLSNLDSFQQLVDNLFGRGNLPSLADAGFNMPATD
                     ISENAEAYSISAELPGIKKEDIKVSLHDGVLAIEAESKSEHEEKGDKQIRTERRYGKF
                     VRRFTLGNNVNEQSVQAKFENGVLKLTIPKQKEPQAPKPKSIPVG"
     gene            complement(2330..>2939)
                     /gene="fnr"
                     /locus_tag="VFX11_15265"
     CDS             complement(2330..>2939)
                     /gene="fnr"
                     /locus_tag="VFX11_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX4940038.1"
                     /translation="KKGEHLFRASDPFASIFAVRSGTIKTYSVTEDGEEQVTGFYLPG
                     EVLGIDGISTNFHSNSAKALETAAVCEIPFDKLEFLSAKIPTLQRHFFQLMSREIQAD
                     QQLIMLLSKKSAEERIAALLLSISSRHGKRGLSPSRFRLPMSRNDIGNFLGLAVETVS
                     RVFTRLQKQEIIFVDGKEVEILNMHELCHMSNGMDDENLKTA"
BASE COUNT          712 a          882 c          742 g          603 t
ORIGIN      
        1 ataccgtcat catgctcgcc agtatcatca agcagtttca tttcaagcgg ctgcagcgca
       61 ccacccataa tcccgcaccc cgtcaacagc aaattctgct cgatatactg cgcaccaacc
      121 gcaacaccga attcgggcag acgcaccagt tcagcgccat ccaatccatt gaggatttcc
      181 ggcgtcaggt tcccctcaga aacagcgccg aattcggccc ttacctcaag cgtgtccatc
      241 agggtgaacg gaacgtgctg actgtcgaga cgccctattt cttcgccatg accgcaggct
      301 cgaccggcga ttacaaatac attccgatca cccgtcgctt caagcgcgag ctcgatcgtt
      361 cggtgtatgc gttctaccac ctgctggaaa ccaactgtcc agaattccgc tctgcgccac
      421 tccagttttt tgtcggctca gcggagggag gccagtcccc cggtggcatt ccgcagggat
      481 ttgtatccgg tttcaactac aagaacctgc cggcattcat ccgacgcaag tttgtggtgc
      541 cctactgggt gttcacccag cccgacatgg acgatcgctt ttacgcgatc ggtcgtttcc
      601 tgacagcaaa aaacagtctg gcgggctttg gcggcttttc gccgctggca atcatgagcg
      661 tcatcaaacg cctgctggca aatatcgaca cgctggaaca ggacgtgcgc aatggcaccc
      721 tgacactgaa ccatccgatt acacccaacc gttacacgcc gacttcgtta cccaccttca
      781 agcccgatgc agcgctggcg caaaaaattt ccgactggcg caacaccgaa cgccctctgc
      841 cagacctgat gcagatattg ctgcccaatc tgaaatacgt tgccacctgg atgggcggca
      901 acatgggtta cagcacacaa tcactgctgc aactgatcgg gaccaagcag gttcatgaaa
      961 tgccattctc cgcgagtgag ggcattttcg gcattccttt ccggcttgat cgtgaaggcg
     1021 gaattgccgc ggtcaccagc cattttctgg aatacatcag ggaacaggat atcgatctgg
     1081 aaaacccgcc ggtgtactgc gcctgggaac tggaacaggg cgaacactat tatcagatca
     1141 tcaccacctc gggcggcctt taccgttaca acatggaaga cctgattcga gtggagggct
     1201 tctacaacaa aacccccgtc gtacagttcg tatcgaaacg ggcacgccag atatccatca
     1261 gcaacgaacg cctcaacgaa aacgacgtca ccgaaagctg ccgccaggca agtgcttcca
     1321 cgggcgtcca tttcgacgaa ttcattttgt ttcccacccg cgccaaccac tactgcctgg
     1381 tagtggagga atgcgaccag gacctgggcc cgttcgtgca ggcgttcgaa gcccgactgc
     1441 gggccctggc aaaagtctat gacatggaac ggaccgcacg cacactgggc cctgtcacgc
     1501 tgctggaaac ccacaggggc caactgcgcg actatacgaa tgccatctac ttccgctctg
     1561 cactgcccag ctcgcagtac aaacccgtcc atctgtcgaa cagcttcacc gatgcggact
     1621 cgtttccggt agcacggttg cattcgcttg aaatatgagc gacgagcatc aatgaatcct
     1681 ttccgttgtc ccagatcgcg cccatttaag gacgattttg acccatatca attgaactcc
     1741 gtcaaatcgg ccctatataa aagatgcgaa acaaaaacat cagttaaagg aggaatttat
     1801 gtcttcattg cgtccctaca acagtccgct gagcaacctg gacagcttcc agcaactggt
     1861 cgataactta ttcggccgag gcaatctgcc gtcattggcg gatgccggct tcaacatgcc
     1921 cgctaccgac atttccgaga acgccgaggc atacagcatc agcgcggaac tgccgggcat
     1981 caagaaggag gacatcaaag tgtcgctgca tgatggcgtg ctggccatcg aagccgagtc
     2041 gaaaagcgag cacgaggaaa aaggcgacaa acagatccgc accgaacgcc gttatggcaa
     2101 attcgtgcgc cgcttcacgc tgggcaacaa tgtgaacgag cagagcgtgc aggcgaaatt
     2161 cgagaacggc gtgctgaagc tgaccattcc caaacagaag gaaccgcaag cgccgaaacc
     2221 gaagtccatt ccagtgggat gatccgtttc gacagcaacg gcctgcccat aaaaaaagcg
     2281 cagcggttga tgaccgctgc gcttcctgaa acttttggag aatggctaat caggccgtct
     2341 tcagattctc gtcatccatg ccattcgaca tgtgacacag ctcgtgcatg ttgagaattt
     2401 ccacttcctt gccatccaca aaaatgattt cctgcttctg caaacgggtg aacacccgac
     2461 tcacggtttc caccgccagc cccagaaaat tgccgatatc gttgcgcgac atgggcagac
     2521 ggaagcggct gggcgacaaa ccccgcttgc cgtgacggga ggaaatgctc agcagcaacg
     2581 cggcaatccg ttcttcggcg ctctttttgc tcaacagcat gatcagctgc tggtcggcct
     2641 ggatctcacg actcatcagc tgaaagaagt gccgctgcag cgtgggaatc ttggcggaaa
     2701 ggaattccag cttgtcgaac ggaatctcgc acacggcagc agtttccagc gccttggcgg
     2761 aattggaatg gaaattggtg ctgatgccgt cgatgccaag cacttcgccc ggcaggtaga
     2821 atccggtcac ctgttcttca ccgtcctcgg tgactgaata ggttttgatg gtgccgctgc
     2881 gcaccgcgaa aatcgacgcg aacgggtcac tcgcgcggaa cagatgctcg cccttcttc
//
LOCUS       DASUEG010000812         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1471080, whole genome shotgun sequence.
ACCESSION   DASUEG010000812 DASUEG010000000
VERSION     DASUEG010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471080"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFX11_15270"
     CDS             complement(<1..1065)
                     /locus_tag="VFX11_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX4940039.1"
                     /translation="MQDRTASLTRALQQRILILDGAMGTMIQGYKLTEQDYRGARFAN
                     HGSDLKGNNDLLSITRPDVIGAIHQAYLDAGADIIETNTFNSTAVSQADYHLEALVYE
                     LNREGAALARRLCDAQTAATPDKPRFVAGVLGPTSRTCSISPDVNRPEYRNVSFDELV
                     ENYTEATRGLIDGGADTILIETVFDTLNCRAAIFAVRKFFRDHNVELPVMISGTITDA
                     SGRTLSGQTVEAFYNSVSHAHLLSIGLNCALGAEQLRPYVEELSRVSGCHVSAHPNAG
                     LPNEFGQYDQTPDEMAEIVKEFATSGFVNIIGGCCGSTPAHIAAIANAVANVKPRELP
                     TLKPVMHLSGLEPFSIDENSL"
     gene            1284..1880
                     /gene="nfuA"
                     /locus_tag="VFX11_15275"
     CDS             1284..1880
                     /gene="nfuA"
                     /locus_tag="VFX11_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HEX4940040.1"
                     /translation="MTDSFIKVTESAQEYLRGLLQKQDSAGMGVRIFVERPGTPHAEC
                     CMAYCAAGEAEPTDTRLDLEGFAAYIDGASVPYLQDAVIDYAKDRMGGQLTFRAPKSK
                     VPQIDDNASVEQKINYILYSEINPQLASHGGNVQLIELLEDDTLAVLKFGGGCQGCGM
                     VDVTLKQGIEKTLLEKVPQLKRVTDTTDHTVRENAYFK"
     gene            complement(1987..3060)
                     /locus_tag="VFX11_15280"
     CDS             complement(1987..3060)
                     /locus_tag="VFX11_15280"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX4940041.1"
                     /translation="MSQPSVDDLNVLSQQLLITPDQLKSELPLTDKAIATVVKGRQDV
                     RNILDRKDPRLFIVIGPCSIHDIEAAKDYARRLKKLSDEVSDTLLLIMRVYFEKPRTT
                     TGWKGLINDPYLNDSFKIEDGLHIARRLLLDLAEMGLPTATEALDPISPQYLQDLISW
                     SAIGARTTESQTHREMSSGLSSAVGFKNGTDGSLTVAVNALQAVSSPHSFLGINDQGQ
                     VSIINTRGNAYAHVVLRGGGGKPNYDSVSVALAEKELAAAKVSQNVMVDCSHANSNKD
                     PGLQPLVMENVTNQILEGNKSIIGLMVESHINFGAQKIPADLSQLKYGVSVTDACIDW
                     ATTEAAVHKMAEKLRDVLPKRNG"
     gene            3230..>3473
                     /locus_tag="VFX11_15285"
     CDS             3230..>3473
                     /locus_tag="VFX11_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940042.1"
                     /translation="MNDTPLNFSACSPETLILTPNRRLSAWLVRDHDAAMAAQGLRAW
                     PRLNALPLDAWLQQLFDALGLLLPAEKSVPRVLTARQ"
BASE COUNT          679 a          969 c         1061 g          764 t
ORIGIN      
        1 cagggagttt tcgtcgatgc tgaacggctc caggcccgac agatgcatta ccggtttcag
       61 tgtcggcagc tcacgcggtt tgacgttggc aaccgcattg gcgatcgccg cgatgtgcgc
      121 tggcgtggac ccgcagcagc cacctatgat gttgacgaaa ccgctggtgg cgaattcctt
      181 gacgatctcg gccatttcgt ccggcgtctg gtcgtactgg ccgaattcgt tgggcaggcc
      241 ggcattggga tgggcactga catggcagcc tgaaacccgg gacagttcct ccacatatgg
      301 ccgcaactgt tccgctccca gggcgcagtt caacccgatg ctgagcagat gggcatggga
      361 taccgaattg tagaaggcct ccaccgtctg gccggacagg gtgcgaccac tggcgtcggt
      421 gatagtgccg gaaatcatca ccggcagttc cacgttgtgg tcgcggaaga atttgcgcac
      481 ggcgaagatg gcggcgcggc agttcagggt gtcgaacacg gtttcgatca ggatggtgtc
      541 ggcgccgccg tcgatcaggc cccgggtagc ttcggtgtag ttctccacca gttcatcgaa
      601 actgacgttg cggtattcag gccggttcac gtccggcgag atcgagcagg tgcggctggt
      661 ggggcccagc accccagcca cgaagcgcgg tttgtcgggg gttgcggcgg tctgggcgtc
      721 gcacaggcgg cgggcgaggg cagcgccttc cctgttcaac tcatacacca gcgcttccag
      781 atgatagtcg gcctgggaaa cagcggttga gttgaaggtg ttggtttcga taatatccgc
      841 gccggcatcc aggtacgcct ggtggatcgc gccgatcaca tcgggacgcg tgattgatag
      901 caggtcgttg tttcccttaa gatcgctgcc gtgattggca aagcgtgcac cccggtaatc
      961 ctgttcggta agcttgtagc cctggatcat ggtccccatg gcaccatcca ggatcaggat
     1021 tcgctgttgc agggctcggg tcaggcttgc ggtgcggtct tggattgatt tcatttttaa
     1081 aatcagactc ttggggttgc gaacgtaaag tccgctattc taacagatcg aggaaatcac
     1141 tcgggcattg cctgcctgag tttttttcat gaaaagggcg attttcttca ttccccgaat
     1201 acccgactgc tgcgcttggt ttttatcccc gaaggcgcta tccttgcagc agtatgacgt
     1261 tcgcgaattc aacaggttgg tttatgacgg acagtttcat caaggttacc gaatcggccc
     1321 aggagtacct gcgcggactg ctccagaagc aggacagcgc cggcatgggt gtgcggattt
     1381 ttgtggagcg tccaggcaca ccccacgccg aatgctgcat ggcgtattgc gcggccggcg
     1441 aggccgagcc aaccgatacc cgcctggatc ttgaaggttt cgcggcctat atcgatggcg
     1501 ccagcgtgcc ctatctgcag gatgccgtca tcgattacgc caaggaccgc atgggtggtc
     1561 agctcacgtt ccgcgcgccc aagtccaagg tgccgcaaat cgacgataac gcttccgtcg
     1621 agcagaagat caattacatc ctctatagcg agatcaatcc gcaactggca tcccacggcg
     1681 gcaacgtgca gctgatcgag ttgctggaag acgacaccct ggccgtgctg aagtttggtg
     1741 gcggctgtca gggttgcggc atggtggatg tgacgctgaa gcagggcatc gagaaaaccc
     1801 tgctggagaa ggtgccccag ctgaagcggg tgaccgatac cacggatcac accgtgcggg
     1861 aaaacgccta cttcaagtga ccggcgcggg ttgaagtcag cctgctggat atagaagaag
     1921 tcagttggaa ataaaaaagg gccttttgaa aaaggccctt tttgttgctc cggaaaacgc
     1981 tgcagatcag ccgttgcgct tgggcaggac gtcccgcagt ttctccgcca tcttgtgtac
     2041 ggccgcttcc gtggttgccc agtcgatgca ggcgtcggtc accgaaacac cgtacttcag
     2101 ctgggacagg tccgccggaa ttttctgggc gccgaagttg atatggcttt ccaccatcag
     2161 gccgataatg gatttgttgc cttccaggat ctggttggtc acgttttcca tcaccaacgg
     2221 ctgcaggccc gggtctttgt tggaattggc gtggctgcag tccaccatga cgttctggga
     2281 caccttggcc gctgccagct ccttttccgc cagcgccacg ctgaccgaat cgtaattcgg
     2341 cttgccgcca ccaccacgca gtacgacgtg ggcataggcg ttgccacggg tattgatgat
     2401 ggacacctgg ccctgatcat tgatgcccag aaaactgtgg gggctggaca ccgcctgcaa
     2461 cgcattcacc gccacggtca ggctgccatc cgtaccattt ttgaagccca cggcagatga
     2521 cagaccgctg gacatttccc ggtgagtctg ggattcggtg gtacgggcgc caatggccga
     2581 ccagctgatc agatcctgca aatactgcgg ggaaatcggg tcgagcgcct cggtggcagt
     2641 gggcaggccc atttccgcca gatccagcag caggcgacgc gcaatgtgca ggccgtcctc
     2701 aatcttgaag ctgtcgttga gatacggatc gttgatcaga cctttccagc cggtggtggt
     2761 gcggggcttt tcaaaataga cgcgcatgat cagcagcagg gtgtcggaca cttcgtcaga
     2821 cagcttcttc aggcgacggg cataatcctt ggcggcttcg atgtcgtgaa tggagcaggg
     2881 gccgatcacg atgaacaggc gggggtcctt gcggtccaga atattgcgca catcctgacg
     2941 gcctttcacc acggtggcga tggccttatc ggtcagcggc agttcggatt tcagttgatc
     3001 gggtgtgatc aacagttgct gcgacaggac gttgagatcg tcgacgctgg gctgtgacat
     3061 tgtatttggg tcctatttca actagctttt gggtatcatc acctgcccga ttctagcgta
     3121 atcaccatac cgatgacagt ttggaaaccc aataatcacg gccacggccg acaataatat
     3181 ggatagagat cactgaacct cccacttcac tcatcggtac cgcattgttg tgaacgacac
     3241 accactgaat ttttccgcct gcagccctga aacgctgatc ctgacgccga accgccggct
     3301 ttccgcctgg ctggtgcgcg atcacgatgc ggcaatggcg gcgcagggcc tgcgtgcctg
     3361 gccccggctc aacgcgctgc cgctggatgc ctggctgcag cagttgtttg atgcgctggg
     3421 cttgctgctg ccggcggaaa aatccgtgcc ccgcgtgctg acggcccggc agt
//
LOCUS       DASUEG010000813         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1976484, whole genome shotgun sequence.
ACCESSION   DASUEG010000813 DASUEG010000000
VERSION     DASUEG010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976484"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1612
                     /locus_tag="VFX11_15290"
     CDS             119..1612
                     /locus_tag="VFX11_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX4940043.1"
                     /translation="MTLALVRTRARVGMNAPEVTVEVHLSNGLPAFNIVGLPDAAVRE
                     SKERVRSAILNSGLEFPQRRITVNLAPADLPKDGGRFDLPIAIGILAASEQIPLPALE
                     RHEFIGELALSGELRPVDGALTASLAAAKQQRSLFVPQHNANEASVPKTSRIHAAQHL
                     MQVCGLLKNPQQLMAFVAAPASDDELQIYPDLSDVRGQHQARRALEVAAAGKHNLLML
                     GPPGAGKSMLAARLPGILPPMTDAEMMEVAAVHSVAQVNARALHQRPIRAPHHTASGA
                     ALAGGGGNPKPGEISLAHNGILFLDEFPEFPRKVLEVLREPLETGEIAISRAAHQVVY
                     PANFQLIAALNPCPCGHPDKPPEGCTNPQLCCNKYQSKLSGPLLDRIDLHIRVDALPI
                     AQLQQQSGGESSAAVRERVTAARLRQLERQGAPNQDLRGELLEKHCALQPEDALFLQL
                     AVEKLGLSARGYHRVLRVARTIADLAGDGGIGKRHLSEALSYRALFS"
     gene            1666..2235
                     /locus_tag="VFX11_15295"
     CDS             1666..2235
                     /locus_tag="VFX11_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940044.1"
                     /translation="MKKAFLMSALLLAGCSSTDMERIAGDAMSAALNGAMNSVTPAGM
                     AQPSTTTASPATQTNQTSGQPASNAVESNNITTSKAARTKQNFGKVLVVKTEKNPSNQ
                     TKLVLDGCSHPRIGNLRCGGYIFETTSEGWLIDKPISFDDLNYPEIVVAAGNYYLKFT
                     NWDSGYNRFTTGDFIVKPFVTNHVVLELE"
     gene            2408..2653
                     /locus_tag="VFX11_15300"
     CDS             2408..2653
                     /locus_tag="VFX11_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940045.1"
                     /translation="MANIHGAIAVCEKRILTRWEKLAQNRAELEILCAEPGKKGLQEE
                     QWVSARLDRANQLLEENRILLQEIEKQYLALLKVRHR"
     gene            2945..>4088
                     /locus_tag="VFX11_15305"
     CDS             2945..>4088
                     /locus_tag="VFX11_15305"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940046.1"
                     /translation="MVPHALAAVSAADTQAGPVLQLDERVVGTLVGEHLRYLEDPAGT
                     LTLKQVQAMPTRFVQSTSAALNFSFTTSTYWFHLRLENRHAAQPDWLLEAAYPLIDHY
                     TFYVLENGQLVDTTVAGRAFPFQKRAIKNRNVVYPLTLLPGQGKEVYIRVQTSSSLQL
                     PLRVWTPLAFHVKDHDEQVIQGIYYGILLSMLVYNFLIFLSIREISYLNYVHFIGSSI
                     LFFVSLSGYGVEYLWQESTRWALTAQPVLISFTLVGMLNFSRSFLSLDKLMPRAARLV
                     RIMAYLMFALTVAPFVLPYAVALKLAVAGALVSALIVLSCGGIALASGARQARYFMLA
                     WTVLLLGISLYALKTFDLVPSNLLTEYAMQIGSAMEAVMLSFALAHR"
BASE COUNT          906 a         1144 c         1196 g          842 t
ORIGIN      
        1 cccttgaccg ggccaaccgg ccgtcaccct cctgagcgcc ctgcacaccg aatcctgtcc
       61 gcgcgaaaac ttgcgcccca cctgtctagt aacggatcgc ttttcataag gacgaaccgt
      121 gaccctggcc cttgtacgca cccgcgcccg cgtgggcatg aacgcaccgg aagtaaccgt
      181 ggaagtacac ctgtccaacg gactgcctgc cttcaacatt gtcggcctgc ccgatgccgc
      241 cgtgcgcgaa agcaaggagc gcgtgcgcag cgcgatcctc aattccggcc tggaatttcc
      301 ccagcgccgc atcacggtga acctggcccc cgccgatctg cccaaggacg gcggccgttt
      361 cgatctgccc attgcgatcg gcatcctggc ggcgtcggaa cagattccgc tgccggcgct
      421 ggagcggcac gaatttatcg gcgagctggc gctgtcggga gaattgcggc cggtagacgg
      481 cgcgctgacc gcatcgctgg ctgcagcgaa acaacagcgc agtctgtttg taccgcaaca
      541 caatgccaat gaagccagtg tgccgaaaac cagccgcatt cacgccgcgc agcatctgat
      601 gcaggtgtgc gggctgctga aaaatccgca gcagttgatg gcgtttgttg cggcaccggc
      661 ttcggatgat gaattgcaga tctatccgga tctgagtgat gtacgcggcc agcatcaggc
      721 caggcgcgca ctggaagtgg cagcggcagg gaaacacaat ttattgatgc tgggtccgcc
      781 gggtgcggga aaatccatgt tggcggcgcg cttgccgggc attctgccgc cgatgacgga
      841 tgcggaaatg atggaagtgg ccgccgtgca ttcggtggcg caggtgaatg caagagcatt
      901 gcatcagcgg ccgatccgcg cgccgcatca caccgcgtcg ggcgccgcat tggcaggcgg
      961 tggtggcaac ccgaaaccgg gggaaatttc gctggcgcat aatggcattt tatttcttga
     1021 tgaatttccg gagtttccgc gcaaggtact ggaagtgttg cgggaaccac tggagaccgg
     1081 cgagattgcc atttcccgcg ccgctcacca ggtggtgtac ccggcgaatt tccagctgat
     1141 cgccgcgctc aatccctgcc cctgcggcca tccggacaaa ccgccggagg gctgcaccaa
     1201 tccacaactg tgctgcaaca agtaccaaag caaactgtcg ggcccgctgc tggatcgcat
     1261 cgacctgcac atccgcgtgg atgcactgcc gattgcgcag ctgcaacagc aaagcggcgg
     1321 cgaaagcagt gcagcggtgc gggagcgggt gaccgctgcc cgactgcgcc agctggagcg
     1381 ccagggcgct cccaatcagg atctgcgtgg agaactgctg gaaaagcact gcgcattgca
     1441 gccggaagat gctctatttc tgcagctggc cgtggaaaaa ctgggactgt ctgcacgggg
     1501 ctatcaccgg gtactgcggg tagcgcgcac aattgcagac ctggcagggg acggcggaat
     1561 tggaaagcgc catttgagtg aggcgctgtc ctatcgggca ttgttcagtt gatacagatt
     1621 gattcaggaa aaaccttcaa ccagcgtaaa acagggagag taaaaatgaa aaaagcattc
     1681 ttgatgtcag cattgctgct agccggatgc tcctcaaccg atatggaaag aattgccggc
     1741 gatgccatgt ctgccgcttt gaacggggcc atgaactcag ttacgcccgc aggcatggcg
     1801 cagccctcga ccacgactgc atctccggca acgcaaacaa accaaacatc gggccagcca
     1861 gcgtcaaatg ccgttgaatc aaacaacata accacaagca aagcagccag gaccaagcag
     1921 aattttggaa aagtcctcgt agtaaaaaca gaaaagaacc caagcaatca gactaaattg
     1981 gtactggacg gctgttctca tccgagaatt ggaaatctgc ggtgtggtgg atacattttc
     2041 gagactacca gcgaaggctg gctgatcgac aaacccattt catttgatga tctgaactat
     2101 ccggaaattg tggtagcggc tggcaactac tatttgaaat ttaccaactg ggattcgggc
     2161 tacaaccggt ttacgacggg cgattttatc gttaaaccgt tcgtaacgaa tcatgtagtg
     2221 ttggaactgg agtaatggaa ctcgaataga aatcaagccc aatcaaataa tcagaccgtg
     2281 cggacgtaac cacccgccac gcgcggtcga tttaacagca ggcattgacc cctatcaatc
     2341 aaaaattccg cttcgcccag gacaatggac gccaatgttt cattttgtgc acaaacagga
     2401 tatcgacatg gcaaatattc acggggcgat tgcggtttgc gaaaaaagaa ttctgacccg
     2461 gtgggaaaag cttgcgcaga accgggcgga actggaaata ctctgcgccg agcccggcaa
     2521 aaagggcctg caggaagagc agtgggtgtc agccagactg gacagggcca atcagttgct
     2581 ggaggaaaac cgaatcctgc tgcaggaaat cgaaaagcag tatctggcgc tgctcaaggt
     2641 tcgccaccgg tgatcggtgg gattctctcg aatccggatt ccacattgat atgacccgct
     2701 agtttggccc ggtagcgaag gcgcacccgg tcaatgcatc caggaaaagt ccctgccgca
     2761 gctggtggat ggcggcaaag gcggctatac atcaacgatg gaaattcgga atcgaatgga
     2821 atgcgctgat ggtttggggt gaaaaatgac cagcgttgcg gtgcccggaa cacgtcagcg
     2881 cccggggaga acctggctgc aggtggccgc atggcatcta ttggcgtggt tcggctgtgc
     2941 cctgatggtg ccgcatgccc tggctgcggt gtcagcggcg gacactcagg ccggcccggt
     3001 actgcagctg gatgagcgtg ttgtcggcac gctggtgggc gagcatctgc gttatctgga
     3061 agacccggca ggtacgctga ccctgaagca ggtgcaggcc atgcccacgc gttttgtgca
     3121 aagcaccagc gctgccctca atttcagttt taccacctcg acctactggt ttcatctgcg
     3181 cctggaaaac cgtcatgccg cgcagcccga ctggctgctg gaagcggcct atccgcttat
     3241 cgatcactac acgttttatg tacttgagaa cggccagctg gtggacacca cggtagcggg
     3301 ccgcgctttc ccttttcaaa agcgtgcaat caagaaccgc aatgtagttt atccgctcac
     3361 cctgctgcca ggtcagggta aagaggtgta tatccgggtc cagaccagca gctccctgca
     3421 gctgccgctg cgggtatgga cgcctctcgc gtttcatgta aaggaccatg acgagcaggt
     3481 catacagggc atctactatg gcatcctgct ctcgatgctg gtgtacaact ttctgatctt
     3541 tctttcgatc cgcgagatca gctacctgaa ctacgtgcac tttatcggca gttcaattct
     3601 gttttttgtg tcgctcagtg gttacggggt cgaatacctg tggcaggaat cgacacgctg
     3661 ggccctgacc gcgcaaccgg tgctgatcag tttcacgctg gtgggcatgc tcaatttcag
     3721 tcgcagcttt ctcagcctgg ataaactgat gccccgtgcc gcgcgtctgg tgcgcattat
     3781 ggcgtatctg atgtttgctc ttactgtggc accattcgtc ttgccttatg ccgtggcgct
     3841 caagctggcg gtagcgggag cattggtgtc tgcgctgatc gtgcttagtt gtggtggcat
     3901 tgcgctggcc agcggcgccc ggcaggcgcg ctatttcatg ctggcgtgga ccgtactgtt
     3961 gctcggcatt tcgctctacg ccctgaaaac ctttgacctg gtacccagca atctgctgac
     4021 ggaatatgcc atgcagatcg gctccgccat ggaagcggta atgctgtctt tcgcactggc
     4081 ccatcgca
//
LOCUS       DASUEG010000814         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1998390, whole genome shotgun sequence.
ACCESSION   DASUEG010000814 DASUEG010000000
VERSION     DASUEG010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998390"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="lapB"
                     /locus_tag="VFX11_15310"
     CDS             <1..983
                     /gene="lapB"
                     /locus_tag="VFX11_15310"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX4940047.1"
                     /translation="VNRDTFETYLVMGNLYRRRGEVDRSIRIHQDLLAKPDLGKLQQA
                     TVRLELARDYLKAGLLDRSERLLKELADEAGSNQLVALEYLLSIYELEKEWHSAVETA
                     EKLGEKGRQVAVNLSHYHCEQAEQAAKLNDLTEARRELKQALATDRNCVRASLLQAKL
                     ETQAGNDKESIKSLRRVLEQDPQFIPETLDLLRQNYTTTEQLSELVKYLFACLEQHPS
                     ISTVLVLSDLVRELQGDKEGAYVLAEYLKKRPTVKGLDRLIRYQMDVTDDGPVKDNLG
                     LLQVFTQRLIQDKPVYRCEKCGFNGKTLHWQCPSCKGWGTVKPIHGIEGE"
     gene            986..1684
                     /gene="pyrF"
                     /locus_tag="VFX11_15315"
     CDS             986..1684
                     /gene="pyrF"
                     /locus_tag="VFX11_15315"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX4940048.1"
                     /translation="MTRTSPVIVALDFPYQEAALELADRLDPAKCRLKVGKELFTREG
                     PAILKALHQRDFQVFLDLKFHDIPTTVAKACAVAADLGVWMLNVHASGGRRMMEAAQE
                     AVEQAGSGTLLIAVTVLTSMERGDLKEIGLDMEPAEQVERLAALAQSCGLRGVVCSAQ
                     EAPLLRQRLGAEFQLVTPGIRPADAAADDQRRTLTPAQAMAAGVNYLVVGRPITQAAN
                     PSQALDGILASLKI"
     gene            1776..2072
                     /locus_tag="VFX11_15320"
     CDS             1776..2072
                     /locus_tag="VFX11_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005327958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX4940049.1"
                     /translation="MHHILILLLCLLSGIPSVSLAEKPGADKNFVILLPININTATEE
                     MLVQALDGIGQKKAQAIIAYREEQGPFTSVEELANVKGIGPGTLDRNAGRMTVK"
     gene            2182..>2496
                     /locus_tag="VFX11_15325"
     CDS             2182..>2496
                     /locus_tag="VFX11_15325"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4940050.1"
                     /translation="MKTRLSQLLSMGHAVLLALLAALAFAVPADAAGNIIPPDQLINN
                     LFNEVTNRVQKDEAKVVADKTHLITIGNEVLVPYVSFQTMAKQILGKNWRKISAEQQK
                     RYT"
BASE COUNT          540 a          757 c          752 g          447 t
ORIGIN      
        1 acgtcaaccg cgacaccttc gaaacctacc tggtgatggg caatctgtac cgccggcgcg
       61 gcgaagtgga ccgttccatc cgcatccacc aggatctgct ggccaaaccc gatctgggca
      121 aactgcaaca ggccaccgtg cggctggaac tggcgcggga ttatctgaag gcgggtttgc
      181 tggaccggtc cgagcgtctg ctcaaggagc tggcggacga ggcgggctcc aaccagctgg
      241 tcgcgctgga atacctgctg tccatctacg aactggaaaa ggaatggcac tctgcggtgg
      301 aaacagcgga aaagctcggt gaaaaggggc ggcaggtggc ggtgaacctg tcccactacc
      361 actgcgagca ggccgagcag gcagccaagc tgaatgatct caccgaagcc cgccgcgaac
      421 tcaagcaggc gctggccacc gatcgcaact gtgtgcgggc cagcctgttg caggcgaagc
      481 tggaaaccca ggccggcaac gacaaggaat ccatcaagtc cctgcgccgg gtgctggagc
      541 aagaccccca attcataccg gaaaccctcg atctgctgcg gcagaattac accactaccg
      601 aacagctcag tgaactcgtg aaatatctgt tcgcctgcct ggagcaacat ccctccattt
      661 ccaccgtgct ggtactgtca gacctggtgc gggaactcca gggcgacaag gaaggcgcct
      721 atgtgctggc ggaatacctg aaaaagcggc ccaccgtgaa agggctggac cgcctgatcc
      781 gctaccagat ggacgtgacc gacgacggtc cggtgaaaga taatctcggc ctgctccagg
      841 tgttcaccca gcgcctgatt caggacaagc cagtctaccg ctgcgaaaag tgcggtttca
      901 acggcaaaac gctgcactgg caatgcccca gctgcaaggg ctggggcacc gtcaaaccca
      961 ttcatggcat cgaaggcgaa taaccatgac ccgtacctcc cccgtcatcg tcgcgctgga
     1021 ctttccctac caggaagccg cgctggaact ggccgaccgg ctcgatcccg ccaaatgtcg
     1081 cctgaaagtg ggcaaggaac tgttcacccg ggaagggcct gccatcctga aggccctgca
     1141 ccagcgggat ttccaggtat tcctcgatct gaaattccat gacatcccca ccaccgtggc
     1201 caaggcctgt gccgtggcgg cggatctggg ggtgtggatg ctgaacgtgc atgccagtgg
     1261 tggtcgccgg atgatggaag cggcacagga ggcggtggag caggccggca gtggcaccct
     1321 gctgatcgcg gtcaccgtac tgaccagcat ggagcgtggc gacctgaaag agatcggcct
     1381 ggatatggag ccggcggagc aggtggagcg gctggcggca ctggcccagt cctgcggctt
     1441 gcggggtgtg gtctgttcgg cccaggaagc accgttgctg cggcagcggc tgggggcgga
     1501 attccagctg gtcaccccgg gcatccgtcc ggccgacgca gcggcggatg accagcggcg
     1561 caccctgaca ccggcgcagg caatggcggc cggtgtgaat tacctggtcg tcggacgccc
     1621 cattacccag gccgcgaatc cgtcccaggc actggacggc atcctggcca gcctgaaaat
     1681 ctgattcgca ctggccggtt cttttccggc tggctgtcta tcgggtaacc gaacccttga
     1741 ttcggccctg attccagcca ccagagagcc agcccatgca ccacatcctc atacttttat
     1801 tgtgtctgct gtccggtatc ccgtccgttt cacttgcgga aaagcctggc gccgacaaga
     1861 atttcgtcat cctcctgcca atcaatatca atacggccac tgaggaaatg ctggtgcagg
     1921 cgctggatgg tatcggccag aagaaggccc aggccatcat tgcctaccga gaagagcagg
     1981 ggccgttcac gtcagtggag gagctggcca acgtcaaggg cattggcccc ggaacactgg
     2041 accgcaacgc cggtcgaatg actgtgaaat agtacagttg cctattcgga ttgaatgtcg
     2101 ataatccggc gtgcagagtt tcgcacaacc gcgcagaagt ttttataatg cgcgctttca
     2161 accgtttatt tggagtcacc ggtgaaaaca cgcctttcgc aacttttgtc catgggccat
     2221 gccgtcctgc tggcgctgct ggccgcgctg gcctttgcag tgccggctga cgccgcgggg
     2281 aatatcattc ctccagacca gctgatcaac aaccttttca acgaggtgac caaccgcgtt
     2341 cagaaggacg aggccaaagt ggttgccgac aaaacccacc tgatcaccat tggcaatgaa
     2401 gtgctggtgc cttatgtgag cttccagacc atggccaagc agatcctggg caagaactgg
     2461 cgcaagatca gcgccgagca gcagaagcgc tacacc
//
LOCUS       DASUEG010000815         6261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1291902, whole genome shotgun sequence.
ACCESSION   DASUEG010000815 DASUEG010000000
VERSION     DASUEG010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291902"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFX11_15330"
     CDS             complement(<1..179)
                     /locus_tag="VFX11_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4940051.1"
                     /translation="MLRVQSPAKLNLFLHITGRRADGYHNLQTLFQFVDFCDTLEFTA
                     TPDGKIQLDPPLPGVP"
     gene            complement(179..859)
                     /gene="lolB"
                     /locus_tag="VFX11_15335"
     CDS             complement(179..859)
                     /gene="lolB"
                     /locus_tag="VFX11_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEX4940052.1"
                     /translation="MTHTHSGLPPLWRAKSLVSTLHWLTLFSLMLLTQGCALLQPSGT
                     GTPPPPDTQVGWLEHVEKLTLLKEWQIKGKIGVRTRDDGGSAYVDWTQSHDSFYIILN
                     GPLGQGTTIVSGNPSGARLEQSDGTYVAESPDQLVQEHTGWEIPINDLLYWIKGLPAP
                     TGKPALTHNALGTLATLEQDGWSLEFDQYVMSLGTLLPQRIRIRKGELRVVVVVKDWL
                     PLAADDKT"
     gene            complement(884..2617)
                     /locus_tag="VFX11_15340"
     CDS             complement(884..2617)
                     /locus_tag="VFX11_15340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4940053.1"
                     /translation="MISRQFFTALSVVALLAVQGCSSLKPTDSAAPEPAPVAETPAVP
                     EPPARSFEKETLYELLLAEFAGKRNRADVALGKYLKQAHETRDPQVVERAAYIARYLG
                     AHQATLDAAMLWIEIEPENPAPRELAATELLRFGKVDEAMGQIDLLMGLNGTVNFEFL
                     LQATRSSNVETRNRVLEKLTEYTQTRNDDKLWFAKGSLEAMNGNHSQAIDDFDEALAI
                     TPSYGNAILGRTQSMMASGNADDALDYLGKKAQEYPRNKRLGVANARYLIQYGKLPAA
                     QQQFVQLVETFPEDGDLLLSLALLSWENKMEEDAIQYFNQLIAMDYRTEEAYLYLARI
                     AESRKQYSEAESYYRKIGSGPNYTLAQIQLALLFSETGRTSEALQVLDAAIASDKREE
                     LKYLLAKSEVLARAERNQDAIEVLNQGLKQHPDDAALLYSRAMLQEKLERFDLMEADL
                     RKVLQLQPNNSAALNALGYTFANRNQRLNEAWELIERAYFLNPNDPAIIDSMGWIKYR
                     MGENSAALDFLRKAYEAFKDQEIAAHLGEVLWVTGNQQEAISVWKQALEENPGSKVLQ
                     NVMKRFNAEQP"
     gene            2880..4148
                     /gene="hemA"
                     /locus_tag="VFX11_15345"
     CDS             2880..4148
                     /gene="hemA"
                     /locus_tag="VFX11_15345"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007018743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX4940054.1"
                     /translation="MVLLAYGINHKTASVDVRERLAFAPDRVMTALAEAREHARLKEV
                     AILSTCNRTELYCSAEAQDSGRLLGWLSRYHALPMDQITGCAYEFWNDAAVRHLMRVA
                     SGLDSMVLGEPQILGQLKSAFAVAQEAQTVGPDLGRLFQHTFSAAKQIRTDTGIGANP
                     VSVAFAAVKLAKHIFSDLKKTSALLIGAGETIELVARHLHEQGVGNMIVANRTLSRAN
                     ALAEEFNARAVTLGDIPLVLHQADIVISSTASPLPILGKGTVEHALKLRKHKPMFMMD
                     IAVPRDIEEEVAELADVYLYTVDDLQQVVEENRRQRQEAAAQAEALVDRCALQFMESL
                     KTLDAVDTIRAYRDRLEELRQAEFERAMQQIRLGANPEEVLQRMSRALINKVMHVPTI
                     QLKQAAEQGRLDQVEWAQMLLGLSDPAKPD"
     gene            4228..5316
                     /gene="prfA"
                     /locus_tag="VFX11_15350"
     CDS             4228..5316
                     /gene="prfA"
                     /locus_tag="VFX11_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX4940055.1"
                     /translation="MKESIQMKLANTSERYDEVSAMLSQPDVISDQNRFRKLSQEFAE
                     IEPVVMCYRDYDKTLKNMAEARNWMNDSDPEMRALGEEELKASASRCEALEIELQKLM
                     LPKDPFDGSNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAETQGWRIEVVSSSDGEHGG
                     YKEIICRVIGDGVYSRLKFESGAHRVQRVPATESQGRIHTSACTVAILPEADEVTEIE
                     VRTEDLRIDTFRSSGAGGQHVNTTDSAVRITHIPSGLVVECQDERSQHKNKAKALTLL
                     KSRLLQHARDKQHKETSEARRNLVGSGDRSERIRTYNFPQGRVTDHRINLTLYRLPEI
                     MEGKMDELLNALSNEYQADQLAALAESA"
     gene            5316..6155
                     /gene="prmC"
                     /locus_tag="VFX11_15355"
     CDS             5316..6155
                     /gene="prmC"
                     /locus_tag="VFX11_15355"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX4940056.1"
                     /translation="MQTVAQALQWAVEELSAVSPADDAQLDAEWLLGHLLGKNRAWLR
                     AFAEQVLAEADVQQYCGWIARRRAGEPVAYIGGCQGFWSFELEVTADTLVPRPDTELL
                     VETALELLDASAKNVVDLGTGTGAIALALKSERPEWQVLATDIDAASLALAQRNAQRL
                     GLAIDVRESCWLRDLGAEKFQLIVSNPPYIRADDPHLAGAGVRFEPLRALVSGVDGLD
                     AIREIVATAPDHLHAGGWLLLEHGYDQGAAVRGLLQARGFHDVETRRDLGGNERVTLG
                     RWF"
BASE COUNT         1151 a         1682 c         2135 g         1293 t
ORIGIN      
        1 gggacaccgg gtagcggcgg gtccagctgg attttgccat cgggggtggc ggtgaattcc
       61 agggtgtcgc agaagtccac gaactgaaac agggtctgca ggttgtggta gccgtctgcc
      121 cgccgccccg taatgtgcag aaacaggttg agcttggcgg gggactggac gcgcagcatc
      181 aggttttgtc atccgccgcc agggggagcc agtccttcac caccaccacc acccgcagtt
      241 cgcccttgcg gatgcggatg cgttgcggca gcagggtgcc caggctcatt acgtactggt
      301 cgaattccag gctccagccg tcctgttcca gggtggccag ggtgccgagc gcgttgtggg
      361 tcagcgccgg ctttccagtg ggggcgggta gccctttgat ccagtagagc aaatcattga
      421 tggggatttc ccagccggtg tgttcctgca cgagctgatc aggcgattcg gcaacataag
      481 tgccatcgga ctgttccaga cgggcgccac tggggttgcc gctgacgatg gtggtgccct
      541 ggccgagggg gccgttcagg atgatgtaaa agctgtcatg tgactgggtc cagtccacgt
      601 aggcgctgcc gccgtcgtcc cgggtgcgga cgccgatctt gcctttgatt tgccattcct
      661 tcagcagggt gagtttttcc acgtgctcca gccagccgac ctgggtgtca ggtggtggcg
      721 gggtgcctgt gccggagggt tgcagcaatg cgcagccctg cgtaagcagc atgagtgaaa
      781 acagggtgag ccagtgcagg gtggagacaa gcgactttgc ccgccacaaa ggtggcaggc
      841 cggaatgagt gtgtgtcaca actgtttcaa ctggggatcg gggttagggt tgctcggcgt
      901 tgaaccgctt catgacattc tgcaacacct tgctgcccgg attttcttcc agggcctgct
      961 tccagacgga aatggcttcc tgctggttgc cggtcaccca gagtacttca cccaggtggg
     1021 ccgcaatttc ctgatccttg aatgcttcgt aggccttgcg caggaaatcc agtgcggcag
     1081 aattttcgcc catacgatac ttgatccagc ccatgctgtc gatgatggcg gggtcgttcg
     1141 gattgagaaa atacgcgcgt tcgatgagtt cccaggcttc gttgaggcgc tggttgcggt
     1201 tggcgaaggt gtagcccagt gcgttgaggg cggcgctgtt gttcggttgc agttgcagca
     1261 ctttgcgcag gtcggcttcc atcaggtcga agcgttccag tttctcctgc agcatggcgc
     1321 ggctgtaaag cagtgctgcg tcatccgggt gttgtttgag gccctggttc agtacctcga
     1381 tggcgtcctg gttgcgttcg gcgcgggcca gcacttcgct cttggccagc agatacttga
     1441 gctcttcccg cttgtcgctg gcaatggcgg cgtcgagtac ctgcagcgcc tcgctggtgc
     1501 ggccggtttc cgagaacagc agcgctaatt gaatttgagc cagcgtgtag ttggggccgc
     1561 tgccaatctt gcggtaatag gattccgcct cgctgtactg cttgcgggat tcggcaatgc
     1621 gtgcaaggta caggtaggct tcctcggtgc gataatccat tgcaatcagc tggttgaaat
     1681 actggatggc gtcctcttcc atcttgtttt cccaggacag cagggccagc gacagcagca
     1741 ggtcgccgtc ctcgggaaag gtttcaacca gttgcacgaa ctgttgctgg gcggccggca
     1801 gtttgccgta ctggatcagg tagcgcgcgt tggcgacacc cagccgcttg ttgcgggggt
     1861 attcctgtgc cttcttgccg aggtagtcga gggcatcatc ggcattgccg ctggccatca
     1921 tggactgggt gcgtcccagg atggcgttgc cataggacgg ggtgatggcg agggcttcgt
     1981 caaagtcatc gatggcctga ctgtggttgc cgttcatggc ttccagcgag cccttggcga
     2041 accagagctt gtcgtcattg cgggtctggg tgtactcggt gagtttctcc agcacgcggt
     2101 tgcgggtttc aacattcgag ctgcgggtag cctgcagcag gaattcgaag ttgacggtgc
     2161 cgttcagtcc catcagcaga tcgatctgtc ccatcgcttc gtccactttg ccgaaccgga
     2221 gcagttcggt ggcggctagt tcgcggggag cgggattttc cggctcgatt tcgatccaca
     2281 gcatggcggc gtcgagtgtg gcctggtggg cacccaggta gcgggcgatg taggcagcgc
     2341 gttcgaccac ttgcgggtcg cgggtttcat gggcctgttt caggtacttg ccaagcgcga
     2401 cgtcggcacg gttgcgcttg ccggcaaatt ccgccagcag cagctcatac agggtttcct
     2461 tttcgaagga acgggcgggc ggctcgggaa cagccggggt ttcggcaacg ggggcgggtt
     2521 cgggcgctgc ggaatcggtg ggttttaggc tggaacatcc ctgtacggca agcagggcga
     2581 cgaccgataa cgccgtgaaa aactgtctgg aaatcataaa tatgcaggcg gacccatggt
     2641 tgtgccagat tcaatcaaat caggaaaggt gcgcgcatgc aaggaaattg tcccgacgcg
     2701 tcgaccctgc cttcaatgat agatgctgaa tcccgtccgg tgttcccttt gtggttgaat
     2761 cggcgggaaa agccgggtcg ccgggtgctg gcggtcaggc ttgggccttg tgttataatc
     2821 ccgccacttt ttgagaggtc gacgctgcgg tcatcgccgc cgcgggttca acgggagcca
     2881 tggttttact cgcctacgga atcaatcata aaaccgcgtc ggtggacgtg cgggagaggt
     2941 tggcgtttgc gccggaccgg gtcatgaccg cattggcgga ggctcgtgaa cacgcgcgcc
     3001 tgaaggaagt cgccatcctg tccacctgca accgcaccga gctgtactgc tctgctgaag
     3061 cgcaggattc cggtcgcctg ctcggctggc tcagccgcta tcatgccctg ccgatggatc
     3121 agatcaccgg ttgcgcctat gaattctgga acgatgccgc cgtgcggcat ttgatgcggg
     3181 tagccagcgg gctggattcc atggtgctgg gtgagccgca gatcctggga caattgaaaa
     3241 gcgcgttcgc ggtggcccag gaagcgcaga ctgtgggccc cgacctgggg cgtctgttcc
     3301 agcacacctt ttccgctgcc aagcagatcc gtaccgatac cggcatcggc gccaatccgg
     3361 tgtcggtggc gttcgcggcg gtgaagctgg ccaagcacat tttctctgac ctgaagaaaa
     3421 ccagcgctct gctgatcggt gccggtgaaa ccatcgaact ggtggcgcgg catctgcacg
     3481 agcagggtgt cggcaacatg atcgtggcca accgcaccct gtcccgcgcc aatgccctgg
     3541 cggaggaatt caatgcccgc gccgtgacgc tgggtgatat tccgctggtg ctgcatcagg
     3601 ccgacatcgt gatttcgtcc accgccagcc cgctgccgat cctgggcaag ggcacggtgg
     3661 aacatgccct caagctgcgc aagcacaaac ccatgttcat gatggatatt gcggtgccgc
     3721 gcgatattga ggaagaagtg gcggagctgg cggacgttta cctttatacg gtggacgatc
     3781 tgcagcaggt ggtggaggaa aaccgtcgcc agcgtcagga ggcggcggcg caggcagaag
     3841 cgctggtgga ccgttgcgcg ctgcagttca tggaatcgct gaaaaccctg gatgcggtgg
     3901 acaccatccg cgcctaccgc gaccggctgg aggaattgcg ccaggccgag ttcgagcgcg
     3961 ccatgcagca gatccggctg ggtgccaatc ccgaggaagt gctgcaacgc atgagccgcg
     4021 ccctcatcaa caaggtcatg cacgtgccga caatccagct gaaacaggcc gccgaacagg
     4081 gccggctgga tcaggtggag tgggcgcaga tgctgctcgg cctgtccgat cccgccaagc
     4141 cggattagcg acgctcgtcg ccctttttcc gtttccggtt ttccgaattt cccccggcgc
     4201 ccgctgcgcc cttgcaggac aggcaacatg aaagaatcca ttcaaatgaa actggccaac
     4261 accagcgagc gctacgatga agtgagcgcg atgctgagcc agcccgacgt aatcagtgac
     4321 cagaaccgct tccgcaagct gtcgcaggaa tttgccgaga tcgagcccgt ggtgatgtgc
     4381 taccgcgact atgacaaaac gctgaaaaac atggcagagg cgcgtaactg gatgaatgac
     4441 agcgatccgg aaatgcgcgc gctgggtgaa gaggagctga aagcgtcggc gtcacgctgc
     4501 gaggcgctgg aaatcgagtt gcagaaactg atgctgccga aagatccgtt cgatggcagc
     4561 aacgtgtttc tggaaattcg cgccggtacc ggtggtgacg aggcggcgat tttctccggt
     4621 gatctgttcc gcatgtattc ccgttatgcc gagacccagg gctggcgcat cgaggtggtg
     4681 agcagcagcg acggtgagca cggcggctac aaggaaatca tttgccgggt gatcggcgat
     4741 ggcgtgtatt cgcggctgaa attcgaatcc ggcgcgcacc gggtgcagcg ggtaccggcg
     4801 acggaatccc aggggcgcat tcatacgtcc gcctgcacgg tggcgattct gccggaagcg
     4861 gatgaagtga ccgagatcga agtgcgcacg gaagatttgc gcatcgatac ctttcgttcg
     4921 tcgggcgccg gcggccagca cgtgaatacc accgattccg ctgtgcgcat cacacatatt
     4981 ccgtcggggc tggtggtgga gtgccaggat gagcggtcgc agcacaagaa caaggccaag
     5041 gcgctgacct tgctgaaatc gcgactgctg cagcatgccc gcgacaaaca gcacaaggaa
     5101 accagtgaag cacggcgcaa tctggtgggc agcggcgatc gttccgagcg gattcgcacc
     5161 tacaattttc cccagggccg ggtgacagac caccgcatca acctgacact gtatcgcctg
     5221 ccggaaatca tggagggcaa gatggatgag ttgctgaatg cgctgagcaa tgaataccag
     5281 gccgaccagc tggcggcgct ggcggaatcg gcctgatgca aacggtcgcg caggcgttgc
     5341 agtgggcggt ggaggagttg tctgccgtga gtcctgcgga cgatgcgcag ctggatgccg
     5401 agtggctgct tgggcatctg ctgggaaaaa atcgggcctg gctgcgggcg tttgcagagc
     5461 aggttctggc ggaagccgat gtccagcaat attgcggctg gattgcgcgg cggcgggccg
     5521 gcgagccggt ggcgtatatt ggcggttgcc aggggttctg gagttttgag ctggaggtga
     5581 cggcggatac gctggtgccg aggcccgata cggaactgct ggtggaaacg gcgctggaat
     5641 tgctggatgc gtcggcgaaa aatgtggtgg atctgggcac cggtacgggt gccatcgcac
     5701 tggcactgaa aagcgagcgg cctgagtggc aggtgctggc gacggatatc gatgcggcca
     5761 gtctggcgct ggcgcaacgc aatgcgcagc ggctgggtct ggcgattgat gtgcgggaat
     5821 cctgctggtt gcgggatctg ggcgcggaaa aattccagct gattgtctcc aatcctccct
     5881 atattcgcgc ggatgatcca cacttggcgg gagcgggtgt gcggttcgag ccgttgcgtg
     5941 cgctggtgtc cggcgtcgac gggctggatg cgattcgcga gatcgtggcg acggcgccgg
     6001 atcatctgca tgcgggtggc tggctgctgc tggagcatgg gtatgatcag ggcgcggcgg
     6061 tgcgcgggtt gttgcaggcg cgcggatttc acgacgtgga aacgcggcgg gatctgggtg
     6121 ggaatgagcg ggtgacgctg ggtcgatggt tttgaagttg aagagtgtga tattggtacg
     6181 aatgaagtga gttccggcaa cgtggtgcaa gaccgtcagc ggccatggat ggccgctgtc
     6241 gagcctacag ggatgtactt g
//
LOCUS       DASUEG010000816         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_562709, whole genome shotgun sequence.
ACCESSION   DASUEG010000816 DASUEG010000000
VERSION     DASUEG010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562709"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFX11_15360"
     CDS             complement(<1..499)
                     /locus_tag="VFX11_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX4940057.1"
                     /translation="MRADFFAATPVTQLRGIGAAQAEKLARLNIFTLQDVLFHLPFRY
                     QDRTRITPIGATRDGSEALIQGRVLLADVVIRKRRSLLCRIEDGTGQITLRFFHFSGA
                     QQKQFQPGAIVRCYGEVRITALGREIAHPEYRVLETPDAPIEAALTPLYPATEGIQQT
                     SLRRLS"
     gene            complement(499..1410)
                     /locus_tag="VFX11_15365"
     CDS             complement(499..1410)
                     /locus_tag="VFX11_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4940058.1"
                     /translation="MTLTELKYIVTLAQERHFRKAAERCFVSQPTLSVAIKKLEDELD
                     VAIFERNRSDVRLTLVGERIVAQARKVLEEAEIVKLLAQEGKGQLNLPLRVGAIFTVG
                     PYLFPHLIRHIKPIAPDMPLILEENYTSALRQKLRNGELDAIIISLPFEEPDVEIHDL
                     YDEPFEVLIPARHPLAQQEKITRKQLEKERILLLGEGHCFREQVLNACPGVARSVDDH
                     SLPQGTSLETLRHMVASGLGLTIVPSSSNDQHIYDEDTLVERPFAGKIPYRRVSIVWR
                     QRFSRPKAIEALVEAITSCSLLGAANL"
     gene            1541..2371
                     /locus_tag="VFX11_15370"
     CDS             1541..2371
                     /locus_tag="VFX11_15370"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940059.1"
                     /translation="MTTVLLAGCGAIGTQLGVQLQQAGYRVIGLRRSAVASPFPLLQA
                     DFTAPLDPALFNEPIHYVVHTATPGERSDAGYEKAYPGSVRHLLAALRGHPVRRFFFV
                     SSTAVYAQDDGSWVDETSPTDPLEFNGVRMVEAERLLQESGVPATSVRFGGIYGPGRN
                     WLLNRVKKGCSVQSQPPKYTNRIHQDDCVGVLQFLLQKNDSGVRLDNCYVAVDDDPAS
                     EETVCRWLAQQLGAPEPVLEIAAADAPQNKRCRNQRLRALGYVFTYPDFRSGYGARLA
                     "
     gene            complement(2379..2693)
                     /locus_tag="VFX11_15375"
     CDS             complement(2379..2693)
                     /locus_tag="VFX11_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX4940060.1"
                     /translation="MQGYQLTFYTHQDKKHGHLPLAEWLLQEAKKLGAPGATLIAAEE
                     GFGHHRKLHAARFFELGDQPVEVVIALPEADAERLFQRLREEKVRVFYVKSPIEFGTT
                     GD"
     gene            complement(2709..3092)
                     /gene="crcB"
                     /locus_tag="VFX11_15380"
     CDS             complement(2709..3092)
                     /gene="crcB"
                     /locus_tag="VFX11_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX4940061.1"
                     /translation="MLNSILAISLGAALGAVLRWMLGTALNALFAPIPPGTLVANLVG
                     GYLIGVAMALFAQHPGLTPEWRLFVITGFLGGLTTFSTFSAEVVTLLQQGRLLWAGGA
                     IAAHLFGSLLLTLLGIATVTLFRTS"
BASE COUNT          593 a          987 c         1057 g          630 t
ORIGIN      
        1 cgctgaggcg gcgcaggctg gtctgctgga tgccttcggt agcggggtac agcggcgtga
       61 gcgcggcttc gatcggcgcg tccggtgttt ccagtacgcg gtattccgga tgcgcgattt
      121 cgcggcccag agcggtgata cgcacttcgc cgtaacagcg cacgatggcg ccgggctgaa
      181 actgtttttg ctgtgcgccg gaaaaatgaa agaagcgcag cgtgatctgg ccggtgccgt
      241 cttcaatgcg gcacagcagg ctgcgccgct tgcggattac cacatcggcc agcagcacgc
      301 ggccctgaat caatgcttcg ctgccgtcgc gggtggcgcc gatcggcgtg atgcgggtgc
      361 ggtcctgata gcggaagggc agatgaaaca gcacgtcctg caacgtgaag atattcagcc
      421 gcgccagttt ttccgcctgt gccgcgccga tgccgcgcag ttgcgtcacc ggtgtggcag
      481 caaaaaaatc cgctctcatc agagattggc tgcgcctaac agcgaacagc tggtgatggc
      541 ttccaccagt gcttcaatgg ctttcggccg cgaaaaacgc tggcgccaga cgatggaaac
      601 gcggcggtag ggaattttcc ccgcgaacgg ccgttccacc agcgtgtcct cgtcgtagat
      661 gtgctggtcg ttggaggaac tgggcacgat ggtaaggccg agcccggacg ccaccatgtg
      721 gcgcaacgtt tccagcgagg tgccctgcgg caggctgtga tcgtccaccg agcgggcgac
      781 gccaggacac gcattcaata cctgttcgcg gaaacagtgg ccttccccca gcagcaggat
      841 gcgttccttt tccagctgct tgcgggtgat tttttcctgc tgcgccagtg ggtgccgcgc
      901 cgggatcagc acttcgaagg gttcgtcgta caggtcgtgg atttccacat ccggctcttc
      961 gaacggcagc gagatgatga tggcgtccag ctcgccgttg cgcagtttct gccgcagtgc
     1021 gctggtgtaa ttttcttcca ggatcagcgg catgtccggc gcaatgggct tgatgtggcg
     1081 gatcaggtga gggaacaggt acggtcccac ggtgaagatg gcgcccaccc gcagcggcag
     1141 gttgagctgg cctttgcctt cctgcgccag cagtttcacg atctcggctt cttccagcac
     1201 cttgcgcgcc tgtgccacaa tgcgctcacc caccagggtc aggcgcacgt ccgagcggtt
     1261 gcgttcgaaa atcgccacat ccagttcgtc ttccagtttt ttgatggcca cgctgagggt
     1321 gggctggctg acgaagcagc gctccgccgc cttgcggaag tgccgttcct gggcgagggt
     1381 gacgatgtat ttgagttcgg tgagagtcat ggcgcgctcg ctgttttgaa ttccggatgc
     1441 gggcacgata gcatacagcc atcaaacgca aaaccggcca agagtttatc tattgcctgg
     1501 ttgccggttg aacccgtttc cctgcacaag gaacttcctg atgactactg ttctgctggc
     1561 cggctgcggc gccatcggta cccaactggg cgtgcagctg caacaggccg gttaccgggt
     1621 gataggactg cgccgctctg ccgtggcgtc accatttccg ctgctgcagg cggatttcac
     1681 cgcaccgctc gatccggcac tgttcaacga accgatccat tatgtggtgc acaccgccac
     1741 gccgggtgag cgcagcgatg ccggttatga aaaggcgtat cccggcagcg tgcgccatct
     1801 gctggcggcg ttgcgcggcc atcccgtgcg gcgatttttt ttcgtttcca gcaccgctgt
     1861 ttacgcgcag gacgatggca gctgggtgga tgaaaccagc cccaccgatc cgctggaatt
     1921 caacggcgtg cgcatggtgg aggcagaacg tttgctgcag gaaagcggcg tgcctgccac
     1981 cagtgtgcgt ttcggcggta tttacggccc cggccgcaac tggctgctca atcgcgtgaa
     2041 aaaaggctgc agcgtgcaga gtcagccacc gaaatacacc aaccgcattc accaggacga
     2101 ttgcgtgggt gtgctgcaat tcctgctgca aaaaaatgac agcggcgtga gactggataa
     2161 ttgctatgtg gcggtggacg atgatccggc cagcgaggaa accgtgtgcc gctggctggc
     2221 gcagcagctg ggggcgcccg aaccggtgct ggaaattgcc gcggcggatg cgccgcagaa
     2281 caaacgctgc cgcaaccagc gcctgcgggc gttgggctat gtgttcacgt atccggattt
     2341 tcgcagcggc tatggtgccc ggctggccta gcgggttgtc agtcgccggt ggtaccgaac
     2401 tcgatgggcg acttgacgta gaacacgcgg accttctctt cccgcagacg ctggaacagg
     2461 cgctccgcat ccgcttccgg caacgcgatc accacttcca ccggctggtc gcccagctcg
     2521 aaaaagcgcg cggcgtgcag tttgcggtgg tggccgaagc cttcttcggc agcaatcagt
     2581 gtggcaccgg gcgcccccag ttttttggct tcctgcagca gccactccgc cagcggcagg
     2641 tggccgtgtt ttttgtcctg gtgggtgtag aacgtcagct ggtaaccttg catggggatg
     2701 tcctccggtc agctggtgcg aaacagggtc acggtggcga tgccgagcaa cgtcagcagc
     2761 agcgaaccga acagatgcgc ggcgatggca ccgcccgccc acagcagccg gccctgttgc
     2821 agcagggtga cgacttcggc ggaaaaagtg gaaaaggtgg tgaggccacc gagaaagccg
     2881 gtgatcacga acaggcgcca ttccggcgtc aggcccgggt gctgggcgaa cagggccatt
     2941 gccacgccaa tcaggtagcc acccaccaga ttggccacca gggtgcccgg gggaatcggc
     3001 gcaaacagcg cattgagcgc ggtgcccagc atccagcgca gcacggcgcc gagggcagcg
     3061 cccaggctga tggcgagaat ggaattcaac atcggtctgt ccttggtcag ggtactggcg
     3121 gtcccggcgg gcggccctgt cggcatacta gcccagcccc ttcccagcgt catccgggta
     3181 tcgcgcgacg ccacgcctga ccgctccgcc caaaaccctg tcactttccg caattgcgtc
     3241 cccgccccga aaggccgata aacggct
//
LOCUS       DASUEG010000817         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2130070, whole genome shotgun sequence.
ACCESSION   DASUEG010000817 DASUEG010000000
VERSION     DASUEG010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130070"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..910)
                     /gene="yrfG"
                     /locus_tag="VFX11_15385"
     CDS             complement(242..910)
                     /gene="yrfG"
                     /locus_tag="VFX11_15385"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HEX4940062.1"
                     /translation="MIHWDAIDTVLLDMDGTLLDLYFDNHFWLHYLPQKYAEANHLSL
                     DAAKTKLFAEFSAHRGTLNWYCLDFWSRQTGLDIVKLKEDVTHHIGARPYALEFLEKV
                     KASGRRAVLVTNAHRGSLELKLQHVPLDLHLDTLISSHDLGLPKEDPHFWTRLEEVLH
                     YDRTRTLLIDDSLAVLRSAKNAGIAFTLGVLQPDSKAGPVNTEEFIALDSFTHIYPPP
                     VHPA"
     gene            958..1527
                     /gene="nudE"
                     /locus_tag="VFX11_15390"
     CDS             958..1527
                     /gene="nudE"
                     /locus_tag="VFX11_15390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEX4940063.1"
                     /translation="MPEKPSILSHDIVAQSRLFGIERLELRFSNGEERVYERLRSPRI
                     PAVLVVALKDDDTVILIREYACGTDDYQLSLPKGAFDHHGEHVFEAANRELMEEAGYG
                     ANKFTELKRLSLSPSYMGHMIHIVLAEDLYEKRLPGDEPEPIEVFEHKLEDIDSLLAA
                     EDFTEARALAALYMVRDIVRARRQQRGQS"
     gene            1524..>2397
                     /locus_tag="VFX11_15395"
     CDS             1524..>2397
                     /locus_tag="VFX11_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4940064.1"
                     /translation="MTTEEDKWLSISIEVMFKLGALLLLLIWCFLILEPFLIMIVWGT
                     IIAVASYPTYDKLRHRFQLTDNVAAVLMGGLLVTAIIVPVLMISDIVIDGSKQLLAQL
                     KQGDATLTLPLLPESVKQWPVVGPKLWDLWSNATHNVMKTLAQFQPQLKAAGKTLLGA
                     AGGVSIAILQFIFSIVVAGVLLANSEAGSKLARRLSRRLVGRDGVDIIGLVSATIRSV
                     TRGILGVAFIQALLAGIGFAVMGVPMAGLLTLLCLILCIVQLGTLLVAAPVVIYVFSV
                     SDTVPAVIFTVYMLI"
BASE COUNT          451 a          655 c          773 g          518 t
ORIGIN      
        1 acaacaggat ggccagggcg tactgcgcgg ctcgtcccgg aaaccctgtc ggtggtttcc
       61 ggtaaatcat ggatggttcc ctctgcgatt attgtttttg tactgcagcc gtcgggattg
      121 ctggcggact cttccagcgt agttcgttgt ggcggccagg ctatggctga gtacgacagt
      181 ttcttgactt tagtgacagg caatagaggt ggccggcagc ggagcacccg gcgattgcgc
      241 ttcaggcggg atgcaccggt ggcggataga tgtgggtgaa gctgtccagc gcgatgaact
      301 cttccgtgtt gaccgggccg gccttgctgt ctggctgcag tacgcccagg gtgaaggcaa
      361 tgccggcgtt tttcgccgag cgcagcaccg ccaggctgtc gtcgatcagc agggtgcggg
      421 tgcggtcgta atgcagcact tcctccagcc gggtccagaa atgcgggtct tccttgggca
      481 ggcccaggtc gtgggagctg atcagggtgt ccaggtgcag atccagcggc acatgttgca
      541 gtttcagttc caggctgccg cgatgggcgt tggtgaccag cacggcccgc cgccccgacg
      601 ccttcacttt ttccaggaat tccagcgcgt acggccgggc gccgatatgg tgggtgacat
      661 cttccttcag tttcacgatg tcgagcccgg tctgccgcga ccagaaatcc aggcaatacc
      721 agttcagagt gccgcgatgg gcgctgaatt cggcgaacag ttttgttttg gcagcgtcga
      781 ggctaagatg gtttgcttcg gcatactttt gcggcaggta gtgcagccag aaatggttgt
      841 cgaaatacag gtccagcagt gtgccatcca tgtccagcaa cacggtgtcg atggcgtccc
      901 agtgaatcat cacgcactac ccttgaattc ttgaacaaac catacggaaa aacaggaatg
      961 ccggaaaaac ctagcatctt gagtcatgac attgtggcac agagccgcct gtttggcatc
     1021 gaacggctgg agctgcgttt cagcaacggc gaggagcgcg tgtacgaacg gctgcgctcg
     1081 ccccggattc cggcggtgct ggtggtggcg ctgaaagatg acgacaccgt gatcctgatc
     1141 cgtgaatatg cctgtggcac cgacgattac cagctcagcc tgcccaaggg cgcgttcgat
     1201 caccacggcg agcatgtgtt cgaggccgcc aaccgcgagc tgatggaaga agccggctac
     1261 ggcgccaaca aattcaccga actgaagcgc ctgtcgctgt cgcccagtta catgggtcac
     1321 atgattcaca tcgtgctggc ggaagatctg tacgagaaac gcctgcccgg tgacgagccg
     1381 gaaccgatcg aggtgtttga acacaagctg gaggacatcg acagcctgct ggcggcagag
     1441 gatttcaccg aggcccgcgc cctggcggcg ctttatatgg tgcgcgacat cgtgcgagca
     1501 cgccgacaac aacgaggaca atcatgacaa ccgaagaaga caagtggctg tccatcagca
     1561 tcgaagtcat gttcaaactg ggtgcattgc tgttgctgct gatctggtgc ttcctcattc
     1621 tggaaccgtt cctgatcatg atcgtgtggg gcaccatcat cgcggtggcc agctatccca
     1681 cgtacgacaa gctgcggcat cgcttccagc tgacggataa cgtggcggcg gtgctgatgg
     1741 gcgggctgct ggtcaccgcc atcattgtgc cggtgctgat gatttccgac atcgtgatcg
     1801 atggctccaa acagttgctg gcgcagctca agcagggtga cgcgacgttg acgctgccgc
     1861 tgttgcctga aagcgtgaag caatggccgg tagtgggacc caaactctgg gacttgtgga
     1921 gcaacgccac ccacaacgtg atgaaaaccc tggcgcagtt ccagccgcag ttaaaggcgg
     1981 cgggcaagac gctgctggga gcggccggcg gtgtttccat tgcgattctg cagttcattt
     2041 tctcgatagt cgtggcgggt gtgctgctgg cgaattccga ggctggcagc aagctggcgc
     2101 gccggttgag ccgacgcctg gtgggccgtg acggtgtcga catcatcggt ctggtgagtg
     2161 ccaccatccg cagtgtgacc cggggcattc tgggtgtcgc gttcatccag gcgctgctgg
     2221 ccggtatcgg tttcgcggtg atgggcgtgc cgatggcggg gctgctgaca ctgctgtgtc
     2281 tgattctgtg catcgtgcag ctgggcacat tgctggtggc agccccggtg gtgatctatg
     2341 tgttttccgt atcggacacc gtgcccgccg tgatttttac cgtgtacatg ctgatcg
//
LOCUS       DASUEG010000818         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_54917, whole genome shotgun sequence.
ACCESSION   DASUEG010000818 DASUEG010000000
VERSION     DASUEG010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54917"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFX11_15400"
     CDS             complement(<1..416)
                     /locus_tag="VFX11_15400"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX4940065.1"
                     /translation="MKKLGVFLSASLLALTAQATDVAGVSIPDELKTVESQPLKLNGA
                     GVREKWMMDLYVGALYLKQHESDAVKIIKTEDAMAIRLQIVSGMITSERMTSSTLEGF
                     VNATHGNTAPIQKEIDAFLSTFKEPIKEGDVFEMVYV"
     gene            883..1632
                     /locus_tag="VFX11_15405"
     CDS             883..1632
                     /locus_tag="VFX11_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX4940066.1"
                     /translation="MKVLVAVKRVVDANVKVRVAANNSGVDLTNVKMAMNPFCEIAVE
                     EAVRLKEKGVTTEIIVVAVGPKEAQEQIRTALALGADRGILVEAADAQPLAIAKILKA
                     IVDQEKPGLVILGKQTIDGDNNQTGQMLAALTGMGQGTFASAVNVADGKVKVTREIDG
                     GLQTVELSLPALVTTDLRLNEPRYASLPNIMKAKKKPLDIKTPADFGVDVTPRVKTLK
                     VEAPAARKAGIKVKTVEELVAKLKNEAKVIQ"
     gene            1629..>2395
                     /locus_tag="VFX11_15410"
     CDS             1629..>2395
                     /locus_tag="VFX11_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4940067.1"
                     /translation="MSVLVYAEHDNASLKAATLNVVAAAQKIGGEITILVAGSGCGAV
                     AQQAAKVAGVAKVLVADNAAYAHQLAENVSALVAEVGKGYKHILAAATTTGKNFLPRT
                     AALLDVQMISEISGVVNATTFQRPIYAGNAIATVESTQAINVLTVRGTAFDPVAAEGG
                     SASIENLSVAKDAGVSSFVGEEIAKSDRPELTAAKIVISGGRGMQNGDNFSILYKVAD
                     KLGAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYVA"
BASE COUNT          532 a          697 c          677 g          489 t
ORIGIN      
        1 acgtacacca tttcgaacac atcgccttcc ttgatgggtt ccttgaatgt ggacaggaac
       61 gcgtcgattt ccttctggat gggtgcagtg ttgccgtggg tggcgtttac aaaaccttcc
      121 agggtcgaac tggtcatgcg ctcgctggtg atcatgccgg acacgatctg caaccggatg
      181 gccatggcat cctctgtctt gatgattttg acggcatcgg actcatgctg cttgagatac
      241 agcgcgccga catacaggtc catcatccat ttttcccgca ccccggcgcc gttcagtttc
      301 agcggctggg attccacggt cttgagttca tccggaatgc tgacaccggc gacatcggtg
      361 gcctgggcgg tcagtgccag aagggaagcg gaaagaaata cacccaattt tttcatgtta
      421 ttgtcctcga caggtcaaag cggtcttcaa ggcgataccg aattctctta ggcggctcgc
      481 caccggcatc ctctgccggc ccgtggataa taccctataa aagagagccg ccgggggcgt
      541 aaaagaggca ttttccatac tgctgtccca tgccgtactg gcagcacccg ggtgcatcgt
      601 ggcggagcag tgccgcccag tcgaaaactg catcggtaaa cccgatcctc aacaggcagc
      661 ttcggtccca ttggaatggc cggaagtgcc ggattctgcc caggaagtgc tattgacctg
      721 tatgtcataa aacggcaaga taccgagcgc tttgaacggg cgattcgctc gtctgccatg
      781 ttcccgacga aagtcgccgt ggtgcagggg attccggccg aacattcttt cggtgcctct
      841 caccgttaaa tccacatcca cctaatttaa gccgaggatt ccatgaaggt tcttgttgct
      901 gtcaaacgag ttgttgacgc caacgtgaag gttcgcgttg ctgcaaacaa cagcggcgtt
      961 gacctcacta acgttaagat ggctatgaac cccttctgcg aaatcgctgt agaagaagcc
     1021 gttcgtttga aagagaaggg ggttactacc gaaatcatcg tggttgcggt aggtcctaaa
     1081 gaagctcaag agcaaatccg cactgcactg gcactgggcg ctgatcgtgg catcctggta
     1141 gaagcagccg acgcgcagcc tctggccatc gccaagatcc tgaaggcaat cgtcgaccag
     1201 gaaaaacccg gcctggtcat cctgggcaag cagaccatcg acggcgacaa caaccagacc
     1261 ggccagatgc tggctgcgct gaccggcatg ggccagggca ctttcgcctc tgcagtcaat
     1321 gttgccgatg gcaaggtcaa ggtcacccgt gaaatcgacg gcggcctgca gacagtggaa
     1381 ctgagcctgc cggctctggt gaccaccgac ctgcgtctga acgaaccccg ctacgcttcc
     1441 ctgcccaaca tcatgaaagc caagaagaag ccgctggaca tcaaaacccc ggctgatttc
     1501 ggtgtggacg tgacgccccg cgtgaagacc ctgaaggttg aagcgcctgc cgcccgcaag
     1561 gctggcatca aggtgaagac agttgaagaa ctggtagcga aactgaaaaa cgaagcgaag
     1621 gtgatccaat gagcgttcta gtatatgcag aacacgacaa cgccagtctg aaggccgcta
     1681 ccctcaacgt ggttgccgct gcccagaaaa ttggtggtga aatcacaatc ctggtagccg
     1741 gttccggctg cggcgccgtg gcccagcaag ctgccaaggt tgccggcgta gccaaggtgc
     1801 tggttgccga caacgctgcc tacgctcacc agctggcaga aaacgtctcc gctctggttg
     1861 cggaagtagg caagggttac aagcacatcc tggccgctgc cactaccacc ggcaaaaact
     1921 ttctgccccg cacggcggcc ctgctggatg ttcagatgat ttctgaaatc tccggtgtgg
     1981 taaacgccac cacgttccag cgtccgatct atgccggcaa cgccatcgcc accgttgagt
     2041 ccacccaggc catcaacgtg ctgaccgtgc gcggtactgc tttcgatccg gtggctgccg
     2101 aaggtggttc tgcttcaatc gaaaacctgt ccgtggcaaa agacgctggc gtttcctcgt
     2161 ttgtgggtga agaaattgcc aagagcgacc gtcccgaact gaccgctgcc aagatcgtca
     2221 tctccggcgg ccgcggcatg cagaacggcg acaacttctc catcctgtac aaggtggccg
     2281 acaagctggg cgcggccgtg ggtgcatccc gcgctgccgt tgatgccggc ttcgtgccca
     2341 acgacatgca ggtcggtcag accggcaaga tcgtcgctcc agaactgtat gttgc
//
LOCUS       DASUEG010000819         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_763133, whole genome shotgun sequence.
ACCESSION   DASUEG010000819 DASUEG010000000
VERSION     DASUEG010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763133"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFX11_15415"
     CDS             <1..589
                     /locus_tag="VFX11_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4940068.1"
                     /translation="NPALLAAVQSTAFDNLEIRFPTLYKDEVLKHAENRGLDPDWVYS
                     VIRQESAFMPAAKSPVGALGIMQIMPATAKLLSRSMRTRAPSLQQLLEPDRNIEMGTF
                     FLKQLSDQFDGNIVLATAAYNAGPGRARAWQPDVTPAPGDIWIETIPFKETRDYVKNI
                     VTYQAIYRHLLGRNVALSRSVAAIPPKYGSETAAR"
     gene            complement(668..1027)
                     /locus_tag="VFX11_15420"
     CDS             complement(668..1027)
                     /locus_tag="VFX11_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEX4940069.1"
                     /translation="MPSFSKPLVCFLLLATAACASKQQVIIDPAGVDMGKYQQDLTEC
                     QQISEQVEQKAAGGAVAGAVVGGLVGAALGNSGTAGRMAGAGAVVGGASGVGATQQEK
                     DQVVKNCMRNRGYSVLN"
     gene            1228..2229
                     /locus_tag="VFX11_15425"
     CDS             1228..2229
                     /locus_tag="VFX11_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940070.1"
                     /translation="MNCLRVLSRRGMPFFILLTTLLLSSCKPVVTFTGQSLLDASGNE
                     YGQLTWNITGETDDEFQLTGVTIEPDIGAVEPQGSLKVYPTETTTYRLTAFASGPNNT
                     VFNTVRTVTIHIGPRADFSLVNDTALRACLKETGFTHLEQFDVIYCLDRNIRSLTGLE
                     QFALTQSVSLDNNAVADLTPLTQMPLLHTVSLSNNGLTSLDALAASDSIRNIAAYNNS
                     ISDPSALAAMPQLLSLALDQNLIADATTLAGLQQLQGLSLARNQITNVTPLGALNGLL
                     ALDISRNGVTTGMTALRTLTDASLIRSEGNGGVRCIDYATLVLALGPVVVFDKCKLF"
     gene            complement(2308..3576)
                     /locus_tag="VFX11_15430"
     CDS             complement(2308..3576)
                     /locus_tag="VFX11_15430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4940071.1"
                     /translation="MMAVSLNPLARIEHNIDVPRSLRDIVRIADNEVNPEWLGLTERD
                     RDRIWGGVEALYRTGAYPALSICIRRHGEILLNRSIGHVRGNGPGDSDPKVIATPDTP
                     YCLFSASKAITAMLVHLLEEQGRINQLNPVSYYIPEFAANGKKRITVQQILAHRAGIA
                     SFKDIDPEMLFDHDAIMNLIYSAKPTNLHGRELAYHALTGGYVLGELVQRVTGASIKE
                     YLRVNVQEPMGFRNFNYGAPDAVYPEVARNYLTGFPVVFPVKQFIKRILGADLQTAID
                     LSNDPRFYQQIIPAGNITATAEECSRFFQCLLNGGEYDGKRIFQPVTIERAVREVSAT
                     QFDSTLLAPMRYSAGMMLGNYPIGLFGPATPSAYGHIGLTNNFCWADPERMIAVSLLT
                     SGNPVLGSHIPRLAMLLTAISRRCKKVFRA"
     gene            3844..>4624
                     /locus_tag="VFX11_15435"
     CDS             3844..>4624
                     /locus_tag="VFX11_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4940072.1"
                     /translation="MASDTSGFNRLVAGVKGALRIAQTTRVLAQTGIEWLKGDRPPTP
                     KLLRQTFERLGTTYIKLGQFIASSPSIFPDSYVEEFQLCLDKTTPIPFHIMKRVMEKE
                     MGKSLETIFAEIDPVPLASASIAQVYAAKLVTGEDVVVKVQKPGVEDVLLTDLNFLYV
                     GAKVLEYFVPNLSMASLSAIVEEIQKGMLAECDFYQEAANIEAFQKFLRETGNDQAVA
                     PKVFKQASTMRVLTMERFYGVPFTDLESIQHIVEDPEAVLIT"
BASE COUNT         1006 a         1466 c         1277 g          875 t
ORIGIN      
        1 caatccggcg ctgctggcgg cggtgcagtc caccgccttc gacaacctgg aaatccgctt
       61 ccccaccctg tacaaggatg aggtgctcaa gcacgcggaa aaccggggcc tggacccgga
      121 ctgggtgtac tcggtgatcc ggcaggaaag cgctttcatg ccagcggcaa aatcgccggt
      181 gggtgcactg ggcatcatgc agatcatgcc ggccaccgcc aaactgctgt cacgttccat
      241 gcggacgcgt gcccccagcc tgcaacagct gctggagccg gaccgcaata tcgagatggg
      301 caccttcttc ctgaagcagc tgtcggacca gtttgacggc aacatcgtgc tggcgacagc
      361 tgcctacaac gccggacccg ggcgggcgcg ggcctggcaa ccggacgtca ccccggcacc
      421 gggcgacatc tggatcgaaa ccatcccgtt caaggaaacc cgcgattacg tgaagaatat
      481 cgtcacctat caggcgatct accgtcacct gctgggacgc aacgtggcac tgtcccgttc
      541 ggtggccgcg attccgccca agtacggcag cgaaaccgca gcgcgctgag cgacctggtt
      601 gtcggatgag ggttcagttc agcaccgaca ctgagttcaa tactggttct tagttcaaga
      661 ctggttctta gttcaaaact gaatagccac gattgcgcat gcagtttttc accacctgat
      721 ctttctcctg ctgggtggcg cccacaccgc tggcgccgcc caccacggcg ccggcaccgg
      781 ccatgcgtcc ggccgtaccg ctgttaccca gcgccgcgcc caccagacca cccaccaccg
      841 caccggccac cgcgccaccg gcggccttct gttccacctg ctcggagatc tgctggcact
      901 ctgtcagatc ctgctggtac ttgcccatgt cgacaccggc cggatcgatg atcacctgct
      961 gcttgctggc gcaggcggcg gtggccagga gcaaaaaaca aacaaggggt ttggaaaatg
     1021 acggcatgga ttcaattcct cagtgcgcgg ggatgaggga ttctaacagg ctgcagaaaa
     1081 ccagcgcgag acctgcacgc tcgtctgctg atagctcgtg ctaatgcata tcatccatta
     1141 cactcaatgc cagcatcgat taggcagttc gatagtcaca cctgcgtcac acgctcatcc
     1201 cataaaaaca acaggagccc agcccccatg aactgcctgc gtgttctttc ccgccgggga
     1261 atgccatttt tcatcctgct gacgacattg ctgttgtcgt cctgcaaacc cgtcgtcacc
     1321 ttcaccggac aatccctgct ggatgccagc ggcaatgaat atggccagct gacctggaac
     1381 atcaccggcg aaaccgacga cgaattccag ctcaccggcg tcaccatcga accggacatc
     1441 ggcgcagtgg aaccgcaagg cagcctgaag gtgtatccga ccgagaccac cacctaccgc
     1501 ctcaccgcct tcgccagcgg ccccaacaac accgtcttca atacggtgcg cactgtcacc
     1561 atccacatcg gcccccgcgc ggacttcagc ctggtgaacg acacggcact gcgcgcctgc
     1621 ctgaaagaaa ccggctttac ccacctggaa cagttcgatg taatttactg tctggaccgc
     1681 aatatccgca gtctgaccgg actggaacag tttgcactga cacaatccgt atcgctggac
     1741 aacaacgccg tggccgatct gaccccgctg acgcagatgc cgctgctgca taccgtcagc
     1801 ctgtcgaaca acgggttgac gtcgctggat gcactggccg cgtcggattc gatccgcaat
     1861 atcgcggcgt acaacaacag catcagcgat ccttccgcac tggcagccat gccgcaactg
     1921 ctgtcgctgg cactggacca gaacctgatt gccgatgcca ccacgctggc gggcctgcag
     1981 caactgcagg gcctgtcgct ggcgcgcaac cagattacca acgtgacacc gctgggtgcg
     2041 ctgaacggat tgctggcgct ggatatttcc cgcaatggtg tcaccaccgg catgacggca
     2101 ctgcgcactc tcaccgacgc cagcctgatc cgcagcgagg gcaatggcgg cgtgcgctgc
     2161 attgattacg caacgctggt gctggccctg ggcccggtgg tggtgttcga caagtgcaag
     2221 ctgttctgat tcgttaaaca ggtcatcgca aaacaaaaac gggaaccgag gttcccgttt
     2281 ttgttggtac aactgaaaca cgttcaatca ggcgcgaaac acctttttgc agcgacgcga
     2341 aatcgccgtg agcaacatgg ccaggcgcgg gatatgcgaa cccagcaccg ggttgccgga
     2401 cgtcagcagc gacaccgcaa tcatccgctc cggatcggcc cagcagaaat tgttggtgag
     2461 accgatatgg ccgtaagccg acggcgtggc cggaccgaac agcccaatcg gataattgcc
     2521 cagcatcatg ccggcgctgt aacgcatggg cgccagcagc gtgctgtcga actgcgtggc
     2581 actcacttcc cgcaccgcgc gctcgatcgt caccggctgg aagatgcgct tgccatcgta
     2641 ttcaccgccg ttgagcagac actggaaaaa gcgcgagcat tcctccgccg tggccgtaat
     2701 gttccccgcc ggaatgatct gctggtaaaa acgcggatcg ttcgacagat cgatcgccgt
     2761 ctgcagatct gcgcccagaa tgcgcttgat gaactgtttc accggaaaca ccacgggaaa
     2821 gccggtcaga taattgcgcg ccacttccgg gtacaccgca tccggtgcac cgtaattgaa
     2881 attgcggaag cccatcggct cctgcacatt cacccgcaga tattccttga tgctcgcacc
     2941 agtgacgcgc tgcaccagct cgcccaacac atagccgccg gtcagcgcgt gataggccag
     3001 ctcacgtccg tgcaggttgg tgggtttggc ggaatagatc agattcatga tggcgtcatg
     3061 atcgaacagc atttccggat cgatatcctt gaacgacgca atgcccgccc ggtgcgccag
     3121 aatctgctgc accgtgatcc gttttttgcc gttggcggcg aattcgggaa tgtaatagct
     3181 gaccggattc agctggttga tcctgccctg ctcttccagc aggtgcacca gcatggcggt
     3241 gatcgccttg gacgcggaga acaggcagta cggcgtgtcc ggtgtggcga tcactttggg
     3301 gtcgctgtcg ccggggccat tgccacgcac atggccgatg gaacggttca gcaggatctc
     3361 gccatggcgg cggatgcaga tcgacagcgc cggataggca ccggtacggt aaagcgcttc
     3421 cactcccccc cagatccggt cccggtcgcg ttcggtaagg cccagccatt cggggttcac
     3481 ttcattgtca gcgatgcgca cgatatcgcg cagggaacgg ggaacgtcga tgttgtgttc
     3541 aatacgggcc agggggttca acgatactgc catcatcact ttgtcgtcgc cgcggcgacc
     3601 acctctgaaa agccaacaga ttcggcaaaa atgtctacaa aatcaggcaa taacagcctg
     3661 attgacgtcc aagcctacgc aaatttatac agagtgtcac ctttaagctt ggacgaattt
     3721 aagggctttg ctctatatca aaacattccg tgtagaatag gccccgtcgg ccccggcccg
     3781 tccgtggcac ggcgtttggg acctttcaag gaagatttac cctgtaatca aggcgttaaa
     3841 ctcatggcat ccgatacttc tggcttcaac agactggtgg ccggcgtcaa gggcgcactg
     3901 cgcatcgccc agaccacccg cgtactcgca caaaccggca ttgaatggct caagggcgac
     3961 cgcccgccca ccccgaaact gctgcggcag accttcgaac gcctgggcac cacctacatc
     4021 aaactgggcc agttcatcgc cagctcgccc tccatcttcc ccgatagcta tgtcgaggaa
     4081 ttccagctct gcctggacaa gaccaccccc atccccttcc acatcatgaa acgcgtgatg
     4141 gaaaaggaaa tgggcaaatc cctggaaacc atcttcgccg aaatcgaccc ggtgccgctg
     4201 gcctccgcct ccatcgccca ggtgtacgcc gccaagctgg tgaccggtga ggacgtggtg
     4261 gtaaaagtgc aaaagcccgg tgtggaagac gtgctgctga ccgacctgaa cttcctgtac
     4321 gtgggcgcaa aagtgctgga gtactttgtg ccgaatctgt cgatggcgtc gctgtcggcc
     4381 atcgtcgagg aaatccagaa aggcatgctg gcggaatgtg atttctacca ggaagccgcc
     4441 aacatcgaag cgttccagaa attcctgcgc gaaaccggca acgatcaagc ggtggcaccg
     4501 aaagtgttca agcaggcctc caccatgcgc gtgctgacca tggagcgttt ctacggcgta
     4561 cccttcaccg acctggaaag catccagcac atcgtggaag acccggaagc cgtactgatc
     4621 accg
//
LOCUS       DASUEG010000820         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1820201, whole genome shotgun sequence.
ACCESSION   DASUEG010000820 DASUEG010000000
VERSION     DASUEG010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820201"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..776)
                     /locus_tag="VFX11_15440"
     CDS             complement(153..776)
                     /locus_tag="VFX11_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4940073.1"
                     /translation="MKNLLKTARITALASALLLPGALLPAAMLVSAPAQAAPEKYVLD
                     TENVHAFIQFKIKHLGFSWLLGRFDTFKGEFTLDPDKIESSSVSVDIDVASLNSNHAE
                     RDKHLRGDDFFAVEKFPKATFTSTRVEKTGEKTANIHGNLTLKGITKPVTLAATYIGG
                     GDDPWGGHRQGFEATTEIVLKDFGIDYNLGPSAQSAQIYISAEGIRK"
     gene            complement(851..1408)
                     /locus_tag="VFX11_15445"
     CDS             complement(851..1408)
                     /locus_tag="VFX11_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX4940074.1"
                     /translation="MEGTWRNTRDRFGWTSILLHWISFLLVLFLLVEGIYMVTLTYYD
                     PLYTTLPHWHKVAGVAIMLLTVGRLLWNRLNPLPAPLPAPRWQQWSARLVHLAFYLLL
                     LALGLTGYVITTAKGKPIDMVFGWQIPALRSWPTDIAEAMGFLHRWVAYGLGALILLH
                     SGVALLHHFVQHDTTLKRMLSPRCK"
     gene            1653..3137
                     /locus_tag="VFX11_15450"
     CDS             1653..3137
                     /locus_tag="VFX11_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4940075.1"
                     /translation="MDSALPGFDYSWRALMESMPVAACMYDVDGQIFWYNSAAVRLWG
                     RHPDPGRDRWCGFVRQYWPDGAPMRPEESPSALILRGKAVESGFEAMGERPDGSKVYF
                     IPQLLALHDRQGNVIGGVNMLLDITARREMEAALRSRETEFRAIIENSPDDIARYDRH
                     CRRIYVNPSLVASVGRTPQQLLGRTVLEDWDLPSARRFHLYLVRVRDKGEGVGFELSW
                     PAGSGEVLHRYVRIVPEFDAEGQVQSMLAVGRDIGAIKQTEQYLRQSRALLRDLAERR
                     EALLEAERKRIAREIHDELGQLLNTLHFSNAALRARLERDNPDLVEKSDAMGGLIQRA
                     VNELRNVATSLRPAALDDGIVCALEWLTQDFSRHTGIACRFVSDREEVDLPEFLAVAL
                     FRIVQESLTNVARHAGASEVTVQLGARRDPLVLEVRDNGKGFDVTVPGRRTSLGLVGM
                     QERALALAGKLDITSEPGLGTCVRASIPLLQTRKPVNDQTTAGG"
     gene            3112..>3317
                     /locus_tag="VFX11_15455"
     CDS             3112..>3317
                     /locus_tag="VFX11_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4940076.1"
                     /translation="MIRLLLADDHTLFREGLKQLLEQHDDIRIVAEASTGGEVLEQLR
                     HQEFDLLLLDISMPGVSGSDLIGRV"
BASE COUNT          623 a          928 c         1106 g          660 t
ORIGIN      
        1 tgtttccatc cgggtttgct tcacgttggt ttgacggctg tggtgggtta cctgttccag
       61 tctagcacag cttgataacg ggtgaagtca ggtgcggcca aggtggggcg gaagttgccg
      121 gcgcggcagc gtgccgcgcc aggcgggtgc gattacttgc ggatgccttc cgcggaaata
      181 tagatctggg cggactgggc ggaggggccc agattgtagt cgatgccgaa atccttcagc
      241 acgatttcgg tagtggcttc aaagccttgg cgatggccgc cccagggatc atcgccgccg
      301 ccaatatagg ttgccgccag cgtcaccggc ttggtgatgc ccttgagcgt caggttgccg
      361 tgaatgttgg cagttttctc accggtcttt tccacgcggg tgctggtgaa ggtggccttg
      421 ggaaattttt ccacggcgaa gaaatcatcc ccacgcaggt gcttgtcgcg ctcggcgtgg
      481 ttggaattca ggctggccac atcgatgtcc acactgacgc tggagctttc gatcttgtcc
      541 gggtccaggg tgaactcgcc cttgaaggtg tcgaagcggc ccagcagcca gctgaatccc
      601 agatgcttga tcttgaactg gataaacgca tggacgttct cggtgtcgag tacgtatttt
      661 tccggtgccg cctgggccgg cgcgctgacc agcatagcag cgggcagcag ggcgccgggc
      721 agcagcaggg ccgaggccag ggcggtgatg cgggcagtct tgagcaggtt tttcatgggc
      781 gcgtcctttg atgttgttgc ggaaacgatt ggttgttttt tccggacagg aacatagcat
      841 gaattgagat tcatttgcat cttggcgaca gcatccgttt cagggtggtg tcgtgctgga
      901 cgaaatggtg cagcagggcg accccgctgt ggagcaggat cagtgccccc aggccgtagg
      961 cgacccagcg gtgcagaaag cccatcgctt cggcgatgtc cgtgggccag ctgcgcaggg
     1021 cggggatctg ccagccgaac accatgtcga tgggtttgcc cttggcagtg gtgatcacat
     1081 agccggtcag gcccagagcc agcaacagca gatagaaagc cagatgcacc agccgggccg
     1141 accactgctg ccagcgcggt gccggcaggg gcgcggggag agggttcagc cggttccaca
     1201 gcaggcggcc gaccgtgagc agcatgatcg ccaccccggc gactttgtgc cagtggggca
     1261 gggtggtgta gagcggatcg tagtaggtga gcgtcaccat atagattccc tccaccagca
     1321 ggaacagcac cagcagaaag gaaatccagt gcagcagaat gctggtccag ccgaaacggt
     1381 cccgggtatt gcgccatgtg ccttccatgc ctaattcctt cgttccatgc cgtcgtgtca
     1441 cctcttcagt atcgagtccg gatctgtgtg cgaccagtgc agcgggctga aggttcaatg
     1501 tccgtgcacc ctggctgact atactgggtg aatcgccagc gtccgcccgc ccggcacact
     1561 ttttccgacc atttaaccat tcaaaaatta aggctatagt ttgaagtcag agaacggatg
     1621 cctgcagcca gaaaattcga cgggacacca gtatggatag cgcactgccc ggctttgact
     1681 attcctggcg cgcattgatg gaatcgatgc ccgttgccgc ctgcatgtac gacgtggacg
     1741 gccagatctt ctggtacaac agcgccgcag tgcgactgtg gggccggcac cctgaccccg
     1801 ggcgtgaccg atggtgcggt ttcgtgcgcc agtactggcc tgacggggcg ccaatgcggc
     1861 cggaagaatc cccgtccgcc ctgattctgc ggggcaaggc tgtggagtcg ggctttgagg
     1921 ccatgggaga gcgtccggat ggcagcaagg tgtacttcat tccccagctg ctggcgctgc
     1981 atgaccggca gggtaacgtg atcggtggcg tgaacatgct gctggacatc actgcgcggc
     2041 gggagatgga agccgccttg cgcagccggg aaaccgagtt tcgcgccatt atcgaaaact
     2101 cgcccgatga cattgcgcgc tacgaccggc actgccgccg catctatgtg aatccttcgc
     2161 tggtggcgtc ggttggcagg acaccgcagc aactgctcgg cagaaccgtt ctggaagact
     2221 gggatttgcc cagtgcccgc cgcttccacc tgtatctggt ccgggtgcgg gacaagggcg
     2281 agggagtggg cttcgaactg tcctggcccg cgggcagcgg tgaagtgctg catcgctacg
     2341 tgcggatcgt gccggaattc gacgccgaag gtcaggtgca gagcatgctg gccgttggcc
     2401 gcgacatcgg ggcgatcaag cagacggaac agtatctgcg ccagtcccgc gccctgttgc
     2461 gggatctggc tgagcggcgg gaagcattgc tggaagcgga gcgcaaacgc attgcccggg
     2521 aaattcacga tgaactgggg cagttgctca ataccctgca tttcagcaac gcggccctgc
     2581 gcgcccggct ggagcgggac aatccggacc tggtggaaaa atccgatgcc atgggcggcc
     2641 tgatccagcg cgcggtgaat gaattgcgca atgtggcgac cagtttgcgc cccgccgcac
     2701 tggatgacgg aattgtctgt gcgctggagt ggctcacgca ggatttcagc cggcataccg
     2761 gcattgcgtg ccgtttcgtc agtgatcggg aagaggtcga tttgccggaa ttcctggccg
     2821 tcgccctgtt ccgcatagtc caggaatccc tgaccaatgt ggcacgtcac gcgggtgcca
     2881 gcgaggtcac cgtacaactt ggcgcgcgcc gtgatccgct ggtgctggag gtgcgcgata
     2941 acggcaaggg atttgatgtg acggtgccgg gacgacgcac ttcgctgggg ctggtgggca
     3001 tgcaggaacg cgccctggcc cttgccggca aactcgacat taccagtgaa cccggcctgg
     3061 gcacctgcgt gcgggccagc attccactgt tgcaaaccag gaaacctgtg aatgatcaga
     3121 ctactgctgg cggatgacca taccctgttc cgggaagggc tgaagcagct gctggaacag
     3181 catgacgata tccggatcgt ggccgaagcc agcaccggcg gcgaagtgct ggaacagttg
     3241 cggcatcagg aattcgacct gttgctgctg gacatcagca tgccgggtgt cagcggcagc
     3301 gacctgatcg ggcgcgt
//
LOCUS       DASUEG010000821         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_916246, whole genome shotgun sequence.
ACCESSION   DASUEG010000821 DASUEG010000000
VERSION     DASUEG010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916246"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..681)
                     /locus_tag="VFX11_15460"
     CDS             complement(133..681)
                     /locus_tag="VFX11_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX4940077.1"
                     /translation="MMNPVFIAKRRAVRWDYALCLTLNRWLEQGPLHLFFRIVSRLGD
                     GLLWYGFMLFLPVFLGGHGFLISLLMLAMASLGLYEYRIIKKRSMRPRPFVTHRKIRQ
                     GARTLDEFSFPSGHTMHAVAFAALLTQAFPWLGFLLIPFALVTGLSRVVLGLHYPSDV
                     FMGAVLGLFNATLFLALFGSWL"
     gene            complement(678..1502)
                     /locus_tag="VFX11_15465"
     CDS             complement(678..1502)
                     /locus_tag="VFX11_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4940078.1"
                     /translation="MSTRNVRAVFISDVHLGTSDCQAQYLLDFLRSHRMDFLYLVGDI
                     VDLLSMRKRVHFTALHEEVVGLVLQLARQGTRVIYIPGNHDARLRRFCGQKFWGIEVH
                     RHYVHACADGRRFFVSHGDEFDSALHAGVFWHVVGDFSHTLLLRLNTLLNGARRLLKL
                     PYWSLAGYLKTRIGKAAEFIRRFEWIAARKARELNYDGYVCGHIHHSGMRRIDGVLYC
                     NDGDWVEHCTALVEERNGQFALVHWADRKEVLLREHELKEHWNPLPVPVTDGGRIV"
     gene            1662..2273
                     /locus_tag="VFX11_15470"
     CDS             1662..2273
                     /locus_tag="VFX11_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940079.1"
                     /translation="MKRCTSRLIFLLLLSLLQACATTQVQESWRLPGYHPPRPQKMLV
                     VALTATETTRKLVENSFVKALEAKGITAIASSTWIADGSRITRDSLVPIVEQNGITTV
                     LVSSMREVQKSAAYQPAAGDDSDGLFRNIDTYYAYSSSGQHEGGSYAQVTEYLIETNL
                     FDGKNRKLSWSVITRTSAPKDLQKGIEGVTGAVMKRAAEDKAL"
     gene            complement(2295..2516)
                     /locus_tag="VFX11_15475"
     CDS             complement(2295..2516)
                     /locus_tag="VFX11_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX4940080.1"
                     /translation="MALFDPSRPQVDPLQSPCVRNCCLDEQDVCLGCGRTLAEIRAWS
                     SLSERERQQVLDLCEERKRQRDRFLQFPR"
BASE COUNT          623 a          882 c          698 g          458 t
ORIGIN      
        1 gtgcttgatc tacatgatcc cagcttattt gtctctgcgc ccgcattcat tgcctcacat
       61 caaggttccg cccctgcacc ccggcgttac ctgcccacgg cattgacctg gagcaacgtt
      121 ctcaagcaac gactacagcc aggatccgaa cagcgccagg aacagcgtcg cgttgaacag
      181 gcccagcacc gcacccatga acacgtcact gggataatgc agccccagca ccacccggct
      241 caggcccgtc accagcgcaa acggaatcag caggaaaccc agccagggga aggcctgcgt
      301 caacagcgcg gcaaacgcta ccgcatgcat ggtatgtccg gatggaaaac tgaattcgtc
      361 cagcgtgcgc gcgccctgcc ggatcttgcg gtgggtcacg aacggacgcg gccgcatcga
      421 acgcttcttg ataatgcggt attcatacag cccgaggctg gccatggcga gcatcagcag
      481 gctgatcagg aaaccatgcc caccgagaaa caccggcaga aacaacatga aaccgtacca
      541 gagcaaacca tcgcccagac ggctgacgat ccggaaaaac aggtgcagcg gcccctgctc
      601 cagccagcgg ttgagcgtca ggcacagtgc gtaatcccag cgcacggcac ggcgtttggc
      661 gataaaaacg ggattcatca tacgatcctt cctccgtctg tcaccggaac cggcagcggg
      721 ttccagtgtt ccttcaattc atgctcccgc aacaacactt ccttgcgatc agcccagtgc
      781 accagggcaa actgcccatt tctttcttct accagcgcag tgcagtgctc cacccagtcg
      841 ccgtcgttgc agtaaagcac accatcaata cgtcgcattc cgctgtgatg gatatggcca
      901 cagacatagc cgtcatagtt cagttcccgc gccttgcggg ccgcaatcca ttcgaacctg
      961 cggataaatt ccgctgcctt gccaatgcgg gtcttcaaat agccagccag cgaccagtag
     1021 ggcagcttca gcaaccggcg cgcgccattg agcagtgtat tcaatcgcag caacaatgta
     1081 tgggaaaaat cccccaccac gtgccaaaaa acaccagcgt gcagtgcgct gtcgaattca
     1141 tcgccatgac tgacgaaaaa ccgcctgcca tcggcgcaag catggacgta atggcgatgc
     1201 acttcgatac cccagaattt ctgtccgcaa aaccgccgca gccgtgcgtc gtgattaccc
     1261 gggatataaa tgacccgcgt gccctgccgc gccagttgca acaccaggcc caccacctct
     1321 tcatgcaggg cggtgaaatg cacgcgcttg cgcatcgata gcagatccac gatatccccc
     1381 accaggtaca ggaagtccat gcgatgacta cgcaggaagt ccagcaaata ctgtgcctga
     1441 cagtcggatg ttcccaggtg aacgtcggaa atgaagacag cacgaacgtt tcttgtactc
     1501 atggcgggcc tcccggttgc cgccagacta gtcccgccgt gcgtaaaaac ctttacagtc
     1561 tcgtgaagaa accgtgacag gcagggacag gtctcgcctc gacaacaacc gcgccaggcc
     1621 ctaccatgcg ccttttcgcc ccaacccagg attcgcctgc catgaaacgt tgcaccagcc
     1681 gtctgatttt tctgctgtta ttgtccctgc tgcaggcttg cgccaccacc caggtgcagg
     1741 aatcctggcg cctgccgggt tatcacccgc cccggccgca aaaaatgctg gtcgtggcac
     1801 tgaccgccac cgagactacc cgcaagctgg tggaaaattc cttcgtcaaa gcgctggaag
     1861 ccaaaggcat caccgccatc gcgtcgtcga cctggattgc cgacggcagc aggatcaccc
     1921 gcgactccct ggtccccatt gtggagcaga acggcatcac caccgtactg gtttcgagca
     1981 tgcgggaagt gcagaagtct gccgcctacc agccggccgc cggtgacgac agcgacggcc
     2041 tgttccgcaa catcgacacc tactatgcct acagcagcag tggccagcat gaaggcggct
     2101 cctacgccca ggtcaccgaa tacctgatcg aaaccaatct gttcgacggc aagaatcgca
     2161 agctgagctg gtcagtgatc acccgcacca gcgcgccgaa agacctgcag aagggcatcg
     2221 aaggcgtaac cggcgccgtg atgaagcggg cagcagaaga caaggcattg taaattctgc
     2281 accagacgac tgcgttaacg cggaaactgg aggaaccggt cccgctggcg tttgcgctcc
     2341 tcgcacaggt caagcacctg ctgacgttcc cgctcgctca ggcttgacca ggcccggatc
     2401 tctgccaggg tgcggccaca gccaaggcag acatcctgct catccaggca acagttgcgc
     2461 acacagggcg attgcaacgg atcgacttgg gggcgggacg gatcaaacag ggccatggtc
     2521 agaaccaccg gggccggcca gcgccacgcg ccacccggtc cacgcaacac cctgccgtgc
     2581 tgaacgcctt cattatcact gccacctcac acctccattc cctcgctgaa gggtagtaca
     2641 gatccagcga cctcaccgga c
//
LOCUS       DASUEG010000822         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_187434, whole genome shotgun sequence.
ACCESSION   DASUEG010000822 DASUEG010000000
VERSION     DASUEG010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187434"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="nhaD"
                     /locus_tag="VFX11_15480"
     CDS             <1..1127
                     /gene="nhaD"
                     /locus_tag="VFX11_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HEX4940081.1"
                     /translation="AMTYINAMEERNIFDALRAWMVRKGFSFRTLFWITGTLAFWISP
                     VADNLTTALLMCAVVLKVGEGDARFINLCCINIVVAANAGGAFSPFGDITTLMVWQAG
                     VVEFGEFFKLLIPSIVNFLVPAVLMSLFIQNRPHHVHAHGDDVHLKRGARRIVILFLL
                     TIVTAVLCHSFLHLPPVLGMMTGLGYLQFFGYYLRVSLPRSLERKRAEAQQRGDDAAI
                     ARLGTVVPFDVFSRIARAEWDTLLFFYGVVMCVGGLGFMGYLAMLSDLLYSNWSATEA
                     NVFIGLLSSVIDNIPVMFAVLNMQPEMSQGDWLLVTLTAGVGGSLLSIGSAAGVGLMG
                     QARGAYTFLGHLRWTPAVALGYIASIMVHLWLNAETFNKF"
     gene            complement(1191..2348)
                     /locus_tag="VFX11_15485"
     CDS             complement(1191..2348)
                     /locus_tag="VFX11_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX4940082.1"
                     /translation="MDVNAMCGWTGRADRLRIWLLGLFWVVSLLLPGASALADETDGQ
                     SLTVFVRTGCPHCADAKEFLAQLQLQRPELQIVYRAVDQDTDARAELLRISRANGLWP
                     PGVPTFVIDGRVRVGFGDPQKSGPELVAFIDQDAAPVQVVETALFGTLNVSQLGLPLF
                     TLALGLLDGFNPCATWVLLFLLSLLVRMKDRARIALVAGTFVLVSGAVYYAFMAAWLN
                     VFLLVGMSNALRIGLAVLALLMGAVNVKDFIAFKKGISLSIPESAKPGLYARMRRVMQ
                     ADALSASLLAVTALAVVVNFVELLCTAGLPAIYTAVLAQQGVSTAGHYAYLALYILAY
                     MADDALMVAIAVIALGSNKMTETTGRWLKLLSGSVMVALGVVMLLKPDWLM"
     gene            complement(2473..>2895)
                     /locus_tag="VFX11_15490"
     CDS             complement(2473..>2895)
                     /locus_tag="VFX11_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4940083.1"
                     /translation="KPFTQRFGAGGRARLNQLQPDGYTRLGAGIRGAGEILKNEAGTP
                     NRLLLVLSDGFPYDDGYESRYAEADAHKALEELRADGVACLCLSIGATTPDVALHRVF
                     GSASHASAANLAELSPRMDALFLSALKELSVSNPRGRR"
BASE COUNT          590 a          890 c          873 g          542 t
ORIGIN      
        1 tggcgatgac atacatcaac gccatggagg aacgcaatat tttcgacgcg ttacgggcct
       61 ggatggtgcg caaggggttc agcttccgca ctctgttctg gatcaccggc acgctggcgt
      121 tctggatttc gccggtggcc gacaacctta ccaccgcgct gctgatgtgc gccgtggtgc
      181 tgaaggtggg tgaaggcgat gcccgcttca tcaatctgtg ctgcatcaat atcgtagtag
      241 cggccaacgc gggcggcgcc ttcagtccgt ttggcgacat cacgacgctg atggtatggc
      301 aggccggggt ggtggaattc ggcgaattct tcaagctgct gattccttcg atcgtgaact
      361 tcctggtgcc ggccgtactg atgagcctgt tcatccagaa ccgcccacac catgtccacg
      421 cgcacggaga tgacgtgcac ctgaaacgcg gtgcgcgcag gattgtgatc ctgtttctgc
      481 tcaccatcgt gactgcagtg ctgtgccact ccttcctgca tctgccgccg gtactaggca
      541 tgatgacagg gctgggttat ctgcaatttt tcggctatta cctgcgggtg tcgctgccca
      601 gatccctgga acgcaagcgc gcggaggccc agcaacgcgg cgacgatgcg gccattgccc
      661 ggctcgggac cgtggtgccc ttcgatgtgt tcagccgcat cgcgcgggcg gagtgggaca
      721 ccctgctgtt tttctacggc gtggtaatgt gtgtgggcgg gctgggtttc atgggctatc
      781 tggccatgct gtcggacctg ctgtacagca actggagcgc cacggaggcc aacgtcttta
      841 tcggactgct gtcgtcggtg atcgacaaca ttccagtgat gtttgccgtg ctcaacatgc
      901 agcccgagat gtcgcaaggc gactggttgc tggtgacgct gaccgccggt gtgggcggca
      961 gtctgctgtc catcggatcg gcggccggtg tcggcctgat ggggcaggca cgcggcgcct
     1021 atacctttct tggccatctg cgatggacac cggcggtggc gctgggttac atcgccagca
     1081 tcatggtgca tctgtggctg aacgcggaga ccttcaacaa attctgaagc atccgtggca
     1141 gcggcgtgca tgcgcgtgtg caggatgttg ccaaaaataa tgggctgcgc tcacatcaac
     1201 cagtccggtt taagcaacat cacaacaccc agtgccacca tgaccgaacc acttagcagc
     1261 ttcagccagc gcccggttgt ttccgtcatc ttgttgctgc ccagtgcaat cacagcaatg
     1321 gccaccatca gggcgtcatc tgccatatag gccagaatgt acagcgccag ataggcgtag
     1381 tgaccagccg tgctgacgcc ctgctgcgcc agtacagcgg tgtagatagc gggcaggccg
     1441 gcggtgcaca gcagctcgac aaaattcacc accaccgcca gcgccgtcac cgccagcaag
     1501 gacgccgaca gcgcatcggc ctgcatcacc cggcgcatgc gcgcatacag accgggcttg
     1561 gcggattcgg gaatcgacag ggaaatgcct ttcttgaacg cgatgaaatc cttgacgttc
     1621 accgccccca tcaacaacgc cagcaccgcc agaccgatgc gcagcgcatt tgacatgccc
     1681 accagcaaaa atacgttcag ccacgcggcc atgaaggcgt aatacaccgc gccactcacc
     1741 agcacaaacg taccggcaac cagtgcaata cgcgcgcgat ctttcatccg caccagtagc
     1801 gacagcagaa acagcagcac ccaggtggca cagggattga aaccatccag caggcccaat
     1861 gccagcgtga acagcggcag gcccagctgc gacacattga gcgtgccgaa caaggcggtt
     1921 tccacgacct gcaccggcgc agcatcctgg tcgatgaatg caaccagctc cggtccgctt
     1981 ttttgtggat cgccgaaacc aacccgtaca cggccgtcaa tgacaaaggt tggcacgccc
     2041 ggtggccaga gtccgttggc gcgggaaatc cgcagcagct cggcacgcgc atcggtgtcc
     2101 tgatccacgg cacggtacac gatctgaagc tcgggccgct gcagctgcaa ctgcgcgaga
     2161 aattccttgg catcggcgca gtgggggcaa ccggtgcgca caaaaaccgt gagcgattgt
     2221 ccgtcggttt catcagccaa cgcactggcg ccaggcagca gcaaactcac cacccaaaac
     2281 agtcccaaca gccagattcg caatctgtct gcgcgccctg tccagccgca cattgcattc
     2341 acgtccatct tcatctccgc cgctattccc tcgctgagca taagacagcc cgcctcaggg
     2401 ccaccttaat ccagatcaga ttaacactgg ttgtcgcccc atggacgaca ggcaaggaga
     2461 aaacgatccc gctcagcgac gaccgcgcgg gttcgagacg gaaagctctt tgagggctga
     2521 gaggaaaagc gcatccatgc gcgggctgag ctcggcgaga ttcgcggcgc tggcgtggct
     2581 ggcggagcca aacacccgat gcaaggcaac gtcaggagta gtggcgccga tggaaaggca
     2641 gaggcaggcg acaccgtcgg cacgcagttc ttccagggcc ttgtgcgcgt ctgcttccgc
     2701 ataacgggat tcatagccgt cgtcgtaggg aaaaccgtcg gacagcacca gcagcagacg
     2761 gttgggcgtg ccggcttcgt tcttgagtat ttcaccggcg ccacggatgc cggcgccaag
     2821 gcgcgtgtag ccgtcgggct gcagctggtt gaggcgggcg cgtccgccgg cgccgaaacg
     2881 ctgcgtgaag ggttt
//
LOCUS       DASUEG010000823         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1750433, whole genome shotgun sequence.
ACCESSION   DASUEG010000823 DASUEG010000000
VERSION     DASUEG010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750433"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFX11_15495"
     CDS             <1..775
                     /locus_tag="VFX11_15495"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX4940084.1"
                     /translation="YPDYEAAQEQGTLIDLFMTSADGIETGTAIRFQGIQVGTIKSVK
                     LKPDLSGVVMQALLHVQPERFARDGSVFWLVQPKLSISGASNLDTLVKGRYFEVIPGT
                     GAAKKQFLGQLEAPVKTGERGGLNLILTTPRLGSIKEGLAVYYREVPVGKVTGYRLGD
                     TADHILVFVNIEPRYTALVRKGSRFWNASGVSIDVGLFKGAKIKTDSLESILAGGIAF
                     ATPDNALALPQAQPGEVFSLSAEMKEEWLAWKPRIPLPK"
     gene            884..1642
                     /locus_tag="VFX11_15500"
     CDS             884..1642
                     /locus_tag="VFX11_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4940085.1"
                     /translation="MFEWIADPSAWVALATLTALEIVLGIDNIIFISILVGRLPEHQR
                     QKARVMGLGLAMFTRIALLMSLAWVMSLTEPLFTIDFLERGFSGRDFILFGGGFFLLW
                     KSTAEIFNALEGEEEAQKQAGKANAFFIILIQIAIIDIVFSLDSVITAVGLSQHVPIM
                     VIAIMIAVFMMMFAAKTIGDFVDRHPSLKMLALSFLILVGFTLMAEGFGVHIPKAYIY
                     VAMGFSLGVEAINIRLRSKRGQPVKLHNQMPEDV"
     gene            1639..3474
                     /locus_tag="VFX11_15505"
     CDS             1639..3474
                     /locus_tag="VFX11_15505"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEX4940086.1"
                     /translation="MTHSFDRVSVAGILSGALDFIPDAPQVARGLLNMLRSSPDRAWS
                     IGLLLEEQARRHPHRPALRFGEREWNYRAFNAWVNRVAALFEKQGVRSGDVVGLLMEN
                     RPEMLVCVAAAVKLGAVAGMLNFNQRDAVLAHSIGLIKPKLMVTSAESVAALETTQYS
                     PLQSTDIWHYWLPNPGSDNKNPERPAGYMDLEQEIRSLPDRNPASTGKVLMKQPCFYI
                     FTSGTTGMPKASVMTHYRWLLGMSGLGLMALRLHADDVLYTPLPLYHNNALTVSWAAV
                     LGSGACLALTRKFSASAFWKEIRQHNATAFCYIGELCRYLLNQPPSQDDRRHRVRVII
                     GNGLRPEIWQEFKQRFGIPRIAEFYGASECNLGFVNAFNLDGTAGFCPLPFAIVACDP
                     ETELPVRDSRGFLQRVPAGGVGLLITEINKRAPYDGYTDDKASEKKRLHNVFAEGDSW
                     FNTGDLVRDQGFRHIQFVDRLGDTFRWKGENVATTEVEAALNQFDQVEQAVVYGVQVP
                     NADGRAGMAALTLRVPLTEFDLQALADTLARQLPVYAIPLFLRMREEHEITGTFKNRK
                     VELKNEGFDPAVVSDPLFYFAGKDAGYRPLDAGVLEQIRDGSLRL"
BASE COUNT          715 a         1156 c         1163 g          710 t
ORIGIN      
        1 gtatccggat tatgaggcgg cgcaggaaca gggcactctg atcgacctct tcatgaccag
       61 tgccgacggt atcgaaaccg gcaccgccat ccgcttccag ggcattcagg tgggcaccat
      121 caaaagcgtc aagctgaaac cggatctcag cggtgtcgtc atgcaggccc tgctgcacgt
      181 gcagccggaa cgtttcgccc gcgatggctc ggtgttctgg ctggtgcagc ccaagctcag
      241 catcagcggc gcctccaacc tggacacgct ggtgaaaggc cgctatttcg aagtgattcc
      301 cggcaccggc gcagcgaaaa aacagttcct cggccagctg gaggcgccgg tcaaaaccgg
      361 cgaacgcggt ggactcaacc tgattctcac cacgccccgg ctcggttcca tcaaggaggg
      421 gctggcggtt tactaccgcg aggtgccggt gggcaaggtc accggttacc ggctggggga
      481 taccgccgat cacattctgg tgttcgtcaa catcgagccg cgctacacgg cactggtgcg
      541 caagggcagc cgtttctgga acgccagcgg tgtcagtatt gacgtgggcc tgttcaaagg
      601 cgcgaagatc aagaccgatt cactggaatc catcctggcg ggtggcatcg cgttcgccac
      661 accggacaat gccctggcgt tgccccaggc ccagcccggc gaggtattca gcctgagcgc
      721 cgagatgaaa gaggaatggc tggcgtggaa accgcgcatc ccgctgccga aatgatgacc
      781 gccccgtcag tcccgccccg gccaaacccg ctttttgccc gcggtttacc tgcggcaggc
      841 caatcgttat cctacgggtc ccttctagta ccgagaccga acaatgtttg agtggattgc
      901 agatcctagc gcctgggtag ccctggcgac cctgaccgca ctggaaatcg tgctgggcat
      961 cgacaacatc atcttcattt ccatcctggt gggccggttg ccggaacacc agcgccagaa
     1021 agcccgcgtc atgggcctgg gcctggccat gttcacccgg attgccctgc tgatgtccct
     1081 ggcctgggtg atgagcctca ccgagccgct gtttaccatc gacttcctgg agcgcggctt
     1141 ctccggccgt gatttcatcc tgttcggtgg cggcttcttc ctgctgtgga agagcaccgc
     1201 agaaatcttc aacgcgctgg aaggcgagga agaggcgcag aagcaggctg gcaaggccaa
     1261 cgcgtttttc atcatcctga tacagatcgc catcatcgac atcgtgtttt cgctggattc
     1321 cgtcatcacc gccgtgggcc tgtcccagca tgtgccgatc atggtgatcg ccatcatgat
     1381 cgcggtgttc atgatgatgt tcgccgccaa gaccatcggc gatttcgtcg accgtcaccc
     1441 ctcgctgaaa atgctggcgc tgagcttcct gatcctggtg ggtttcaccc tgatggcgga
     1501 aggctttggc gtgcatattc cgaaggccta catctatgtc gcgatgggct tctcgctggg
     1561 cgtggaggcc atcaacatcc gcctgcgctc caagcggggc cagccagtga agctgcataa
     1621 ccagatgccg gaagacgtat gacccactca tttgaccgcg tttccgttgc cggaatcctg
     1681 agtggcgccc tggacttcat cccggatgca ccccaggttg cgcgcggtct gctcaacatg
     1741 ctgcggtcca gtccggaccg cgcatggtcc atcggtctgt tgctggaaga acaggcccgg
     1801 cgccatccgc atcggccggc gctgcgcttc ggtgagcgcg aatggaatta ccgcgccttc
     1861 aacgcctggg taaaccgggt ggcagcactg ttcgaaaaac agggtgtgcg cagcggcgac
     1921 gtggtgggcc tgctgatgga gaaccgcccg gaaatgctgg tgtgtgtggc ggcggcggtg
     1981 aagctgggcg cagtggccgg catgctcaat ttcaaccagc gcgacgcggt gctggcccac
     2041 agcatcggcc tgatcaaacc caaactgatg gtgacgtcgg ccgagagcgt ggcggcgctg
     2101 gagacgaccc agtacagccc gttgcagagc accgatatct ggcattactg gttgcccaac
     2161 cctggttctg ataacaaaaa tccggaacgg cccgccggct atatggatct ggagcaggaa
     2221 atccgctcgc tgccggaccg caatcctgcc agtaccggca aagtactgat gaagcaaccc
     2281 tgtttctaca tcttcacctc gggtaccacc ggcatgccca aagcgtcggt gatgacccat
     2341 taccgctggc tgctgggcat gtcgggcctg ggcctgatgg cgctgcgcct gcatgcggat
     2401 gatgtgctgt acacgccgct gccgctgtat cacaacaatg cgctcacggt gagctgggcg
     2461 gccgtgctgg gcagcggtgc ctgcctggcg ctgacgcgca aattcagcgc ctcggcattc
     2521 tggaaggaaa tccgccagca caacgccact gcgttctgct atatcggcga gctgtgccgt
     2581 tacctgctga accagccgcc gtcgcaggat gaccgccggc atcgcgtgcg cgtgatcatc
     2641 ggcaacggtc tgcggccgga gatctggcag gaattcaagc agcgcttcgg tattccacgc
     2701 attgccgaat tctacggtgc cagtgaatgc aacctgggct tcgtcaatgc cttcaacctg
     2761 gacggcaccg ccggcttctg cccgctgccg tttgccatcg tcgcctgcga tccggaaacc
     2821 gaactgccgg tgcgcgacag ccgcggattt ctgcagcggg tgccggccgg tggcgtgggt
     2881 ctgctgatca ccgagatcaa caagcgggcg ccgtacgacg gctacaccga cgacaaggcc
     2941 agcgagaaaa aacggctgca caacgtcttt gccgagggtg acagctggtt caacaccggc
     3001 gatctcgtgc gcgaccaggg ttttcgtcat atccagttcg tggaccggct gggcgacacc
     3061 ttccgctgga aaggcgagaa cgtggccacc acggaagtgg aagccgcgct caaccagttc
     3121 gaccaggtgg aacaggcggt ggtgtacggc gtgcaggtgc ccaacgctga tggccgtgcc
     3181 ggcatggcgg cgctgacact gcgggtgcca ctgacagaat tcgatctgca ggcactggcg
     3241 gatacgctgg cgcggcaact gccggtctat gccattcccc tgttcctgcg catgcgcgaa
     3301 gaacacgaga tcaccggcac cttcaagaac cgcaaggtgg aactgaaaaa cgaggggttc
     3361 gatccggcgg tggtgagcga tccgctgttt tacttcgccg gtaaagatgc cggttaccgg
     3421 ccgctggatg ccggtgtgct ggagcagatt cgggacggca gcctgcggtt gtaggcagcc
     3481 tgccaaatcg gagcccgctg gattattcga agctggacac gaactggccg tccgcccgat
     3541 aaacccgtcc ctgggggctg ctccgcggca tccatgccgc agagcgtccg gcttcgacta
     3601 atccagcggg ctccgattta cactgtcgcg ctcagcgccg ccagaacgcc ggatgcaaca
     3661 acaccagcac ggtgaaaaat tccagccgcc ccaggatcat ggtggcgctg agaatccagg
     3721 tggcgctgtc gctgagcgga atga
//
LOCUS       DASUEG010000824         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_430283, whole genome shotgun sequence.
ACCESSION   DASUEG010000824 DASUEG010000000
VERSION     DASUEG010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430283"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..653)
                     /locus_tag="VFX11_15510"
     CDS             complement(114..653)
                     /locus_tag="VFX11_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940087.1"
                     /translation="MTQLRQEQISAASAFAETSLSLLGSVPGMTTDTLILSAARMAGT
                     FLFRSYAHALNATAPGTVLLSQVASESGPRLVGLLTSALDRLGIRIDTEKVDLNRDNT
                     PVPVLSFLDSQRLLEPALLPVRERFSLSHEEAAQAMAVATAMLIKHQETSLQPQTAFG
                     LAVYGFIEGTKTVPQPILH"
     gene            complement(770..1012)
                     /locus_tag="VFX11_15515"
     CDS             complement(770..1012)
                     /locus_tag="VFX11_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX4940088.1"
                     /translation="MKLLVRNLSRDTTEAELKALFADIGQVTACTLVMDKATGKSKGF
                     GFVEMPKHIEAKTAIKALNAKKIAGSVIRVKEAGED"
     gene            complement(1070..1342)
                     /locus_tag="VFX11_15520"
     CDS             complement(1070..1342)
                     /locus_tag="VFX11_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HEX4940089.1"
                     /translation="MAKAPHTGSNDPLHGLTLETLLRRLVRLYGWPELSRFIRIRCFS
                     DNPSVKSSLTFLRRTPWARQQVEDFYVAYGHGDWLESGLSDDDAAN"
     gene            complement(1374..2048)
                     /locus_tag="VFX11_15525"
     CDS             complement(1374..2048)
                     /locus_tag="VFX11_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940090.1"
                     /translation="MNRPELIDTAPIPNNGGELRLFREGPHFAIKIAGGGELMHSRTH
                     GSEDKLAEITCTRIANRPQPRVLIGGLGMGFTLAAALKHLGSTAEVVVAELVPAVVEW
                     NRGPLGEHAGNPLQDPRASVRVGDVALVLKSEKQAFDAILLDVDNGPQGLTHKSNDWL
                     YSLPGLEAAMSALRPGGVLAIWSAGPDPMFTIRLKKARFLVEEVRVRAHGNKGARHLI
                     WLAWKR"
     gene            complement(2224..3729)
                     /locus_tag="VFX11_15530"
     CDS             complement(2224..3729)
                     /locus_tag="VFX11_15530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4940091.1"
                     /translation="MSIEHFDVLIVGAGLSGIGAAVHLTKECPGKTYAILESRNAIGG
                     TWDLFRYPGIRSDSDMYTLGYSFKPWTNPKAIADGHSIREYIRETSRENGIDKHIRFG
                     HKIIKSAWSTEDAVWTVDIELTGTGKTVQYTCNFLFMCTGYYKYDAGYTPHFEGRDRF
                     KGQIIHPQLWPENLDYSGKKVVIIGSGATAVTLLPAMTDKAAHVTMLQRSPTYVISIP
                     ERDPIANSMRRFLPEKLVYRVTRAKNVSLTLGFFNLAKKRPQAIRRLLLAQVRAQVGK
                     DFDMKHFSPSYNPWDERLCAVPNGDIFKVLRKGKGSIVTDHIETFTETGIQLKSGQHL
                     DADIIITATGLDLQIMGGMQVSMDGKPVDMAQRMNYKGIMFEGIPNIAMIFGYTNASW
                     TLKADISSEYVCRLLKTMDKKGLRQCTPRNTDPSVQVENFVDMRSGYIQRALAKLPKQ
                     GNKAPWKVYMNYFLDLPMLRYSPIDDGSMILSNPKSAARSSGKAKAKAKAA"
     gene            complement(3920..>4516)
                     /locus_tag="VFX11_15535"
     CDS             complement(3920..>4516)
                     /locus_tag="VFX11_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4940092.1"
                     /translation="WLPRGFILVSTNYRLLPDAAPLEQARDVARAVARTQALATEWGG
                     DPERVVLMGHSAGAHLVALLNANPAEALALGARPWRGAVALDSAAMDIRPIMEARHPR
                     FYDKAFGNVPSNWDRVSPLRQLVSGAPPLLAVCSSQRSDSCRQAEGLQARAQTLGVRI
                     EVQPQDLSHGEINGRLGLPGPYTAAVTAFVDSLLPAQP"
BASE COUNT          786 a         1284 c         1525 g          921 t
ORIGIN      
        1 ggggggatga gggctgtgct tctgcccggc agtcagagcc cgagacaatc atgctgcgtg
       61 agcaggcaga gcgctggtgg cagcagctga cgccagcaca gggtttgtaa gcctcagtgc
      121 aggatgggct gtggcaccgt ctttgtgccc tcaatgaagc cgtagacagc gagaccgaag
      181 gctgtctgcg gctgcagcga cgtttcctga tgtttgatca gcatggcggt agcgacggcc
      241 atggcctgtg cggcttcttc gtgactcagg ctgaaacgtt cacgcaccgg cagcagcgcc
      301 ggctccagca ggcgctgcga atccagaaag gacagcaccg ggaccggcgt gttgtcccgg
      361 ttcaggtcca ccttctccgt atcaatgcga atgcccagac ggtccagcgc actggtcaac
      421 aggccgacca ggcgggggcc actctcgctg gccacctgcg acagcagcac cgtgcccggt
      481 gccgtcgcat tcagtgcatg cgcatacgag cgaaagagaa aggtgccggc catgcgggcg
      541 gcgctcagga tgagggtgtc ggtcgtcatg ccggggacgc tgcccagcag cgagaggctg
      601 gtttcggcaa aagcggaggc ggcggaaatc tgttcctggc gcaactgggt catgacatgt
      661 ctcggcgggg ttgatggcgc attgtagcgg gcatggccca tttggtttga tacaccgcaa
      721 gggtggtgat ctgctgcgag gagtattcca cccgcatgcc tgatgaggat caatcctcgc
      781 cggcttcttt tacccggata acgctgcctg cgattttttt cgcattcagc gccttgatgg
      841 ccgtttttgc ttcaatgtgt ttcggcattt ccacaaagcc gaagcccttg gatttccccg
      901 tggccttgtc catgaccagg gtgcaggccg ttacttgccc gatgtcggca aagagtgcct
      961 tgagttccgc ttccgtggtg tcgcgggaga ggttgcgtac cagtagtttc atgagtatgt
     1021 ccgtagcacg gtgtggtgag gcctttcgcc agaaaatgcc ccggtggttt tagttggcag
     1081 catcgtcgtc cgaaaggccg gattccagcc agtcgccatg gccgtaggcg acatagaagt
     1141 cctcgacttg ctggcgcgcc catggcgtgc gtcgcaagaa ggtcaggctc gacttcacgc
     1201 tggggttgtc actgaagcag cggatgcgga tgaagcggga aagctcgggc cagccgtaca
     1261 gccggaccag tcgacgcagc agggtttcca gcgtgagacc gtgcaacgga tcattgctgc
     1321 cggtatgcgg ggccttggcc atgggggctc ctgtggtgtc ggcgttccgg gtgtcagcgc
     1381 ttccaggcca gccagatgag gtggcgagcg cccttgttgc catgcgcccg taccctcacc
     1441 tcttccacca ggaagcgggc ctttttcagg cggatggtaa acatcggatc aggcccggcc
     1501 gaccagatgg ccagcacgcc tccggggcgc aaggcggaca tggcggcttc cagtcccggc
     1561 agcgagtaca gccagtcatt gctcttgtgg gtcaggccct gcgggccgtt gtccacgtcc
     1621 agcagtatgg catcaaatgc ctgcttttca cttttcagga ccagtgccac atcacccacc
     1681 cgcacactgg cacgcggatc ctgcagcgga ttgcccgcat gttcaccgag cgggccgcgg
     1741 ttccattcca ctaccgcagg taccagttcg gccacgacta cttccgccgt gctgcccaga
     1801 tgcttgaggg cggccgccag cgtaaagccc atgcccagtc cgccgatcaa tacccggggc
     1861 tgagggcggt ttgctatgcg cgtgcaggtg atttcggcca gcttgtcttc cgagccatgg
     1921 gtgcgcgagt gcatgagctc gccgccgcca gcgatcttga tggcgaaatg agggccttca
     1981 cgaaacaggc gcagttcgcc gccattgttc gggatgggag cggtatcgat gagttcgggg
     2041 cggttcatgg ggctcggcag gaaagggtgg caaggctgga agggaagcaa tgtgcaaagg
     2101 gtaaggggtt tcgggtgtcg accccagcga tttctgcttg ccggcctgcc gggcgcccag
     2161 aaaaggaaaa cccggacggg gccgggtttt ccttttttgc agggaaggtt gtgcaggctg
     2221 cggtcaggcg gctttcgcct tggccttggc cttgccactg ctgcgagcag ccgacttcgg
     2281 gttggacagg atcatgctgc cgtcgtcaat cgggctgtaa cgcagcatcg gcaggtcgag
     2341 gaagtagttc atgtacacct tccacggcgc cttgttgccc tgcttgggta gcttggccag
     2401 tgcacgctgg atgtagccgg agcgcatgtc gacaaagttc tcgacttgca ccgacggatc
     2461 ggtgttgcgc ggggtgcact ggcgcaggcc cttcttgtcc atggtcttga gcagacggca
     2521 gacatactcg ctgctgatgt cggctttcag cgtccagctg gcgttggtgt agccgaagat
     2581 catcgcgatg ttgggaatgc cttcgaacat gatgcccttg tagttcatgc gctgcgccat
     2641 gtcgaccggc ttgccgtcca tggatacctg catgccgccc atgatctgca ggtccagacc
     2701 ggtagcggtg atgatgatgt ccgcatccag atgctggccc gacttcagct ggatgccggt
     2761 ttcggtaaag gtctcgatgt ggtcggtcac gatggagccc ttgcccttgc gcagcacctt
     2821 gaaaatgtcg ccattgggca cggcacacag acgctcgtcc cacggattgt aggaggggct
     2881 gaagtgcttc atgtcgaagt ccttgccgac ctgggcacgc acctgcgcca gcagcaggcg
     2941 gcggatggcc tgggggcgtt tcttggccag gttgaagaag cccagcgtga gtgacacgtt
     3001 cttggcgcgg gtcacacggt acaccagctt ttccggcagg aagcggcgca tgctgttggc
     3061 aatcgggtcg cgctccggaa tcgagatcac ataggtgggc gagcgctgca gcatggtgac
     3121 gtgcgcggcc ttgtcggtca tggccggcag cagggtcacg gcggtggcgc cactgccgat
     3181 gatcacgacc ttcttgccgc tgtagtccag gttttccggc cacagttgcg ggtggatgat
     3241 ctggcccttg aagcggtcac gtccctcgaa gtgcggcgta tagccggcgt cgtacttgta
     3301 gtagccggtg cacatgaaga ggaagttgca ggtgtactgc acggtcttgc cggtgccggt
     3361 gagctcgatg tccacggtcc agacagcatc ctcggtggac caggccgact tgatgatctt
     3421 gtggccaaag cggatgtgct tgtcgatgcc gttctcgcgg gacgtctcgc ggatgtattc
     3481 gcgaatggag tggccgtcgg cgatggcttt gggattggtc cagggcttga agctgtaacc
     3541 cagcgtgtac atgtccgagt cggagcggat gccggggtag cggaacaggt cccaggtacc
     3601 gccaatggcg ttacggcttt ccaggatggc gtaggtcttg ccggggcact ctttcgtaag
     3661 gtgcacggcg gcgccgatac cggaaaggcc cgcaccgaca atcagtacat caaagtgttc
     3721 aatcgacata agcagtatcc agggagggtt gatcttgcct tacggagccc atcctagtgg
     3781 ccccattcag ggggcgggag gtgtgtggcg gccaatttcc aatcatttcg ggccatcagg
     3841 tgcccggcgt cggacagaaa agacggtttt gtctgacaat ggctgctgtc agatcgtttg
     3901 gttactagca gggagatcgt cagggttggg ccgggagcag gctgtccaca aaggctgtga
     3961 cggctgccgt ataggggccg ggcaagccta gtcgcccgtt gatctctcca tgggacaagt
     4021 cttgcggctg tacctcgatg cggaccccca gcgtttgggc gcgggcctgc agtccttcag
     4081 cctggcggca ggagtcgctg cgttgggacg agcagacagc cagcagcggg ggtgccccgg
     4141 agaccaactg acgcagcggg gagacgcgat cccagttcga cgggacgttg ccaaaggctt
     4201 tgtcatagaa gcgcggatgg cgcgcttcca tgattggccg gatgtccatc gcggcactgt
     4261 ccagcgccac ggcgccgcgc caggggcggg ctcccagcgc cagtgcctcg gcgggattgg
     4321 cgttcagcag tgcgaccagg tgggcgccgg ctgagtgacc catgaggacg acccgctccg
     4381 gatctccgcc ccattccgtt gcgagcgcct gtgttcgcgc gacggcgcgt gcaacgtctc
     4441 tggcctgctc cagtggcgcg gcatcgggca gtagccggta gttggtcgag acaagaatga
     4501 agccgcgcgg cagcca
//
LOCUS       DASUEG010000825         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_99019, whole genome shotgun sequence.
ACCESSION   DASUEG010000825 DASUEG010000000
VERSION     DASUEG010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99019"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFX11_15540"
     CDS             complement(<1..138)
                     /locus_tag="VFX11_15540"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX4940093.1"
                     /translation="MPPSPPLANPPIVLVLAGHDPSGGAGIQADIETLTSLGCHPCTV
                     IT"
     gene            400..819
                     /locus_tag="VFX11_15545"
     CDS             400..819
                     /locus_tag="VFX11_15545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4940094.1"
                     /translation="MIASHVMFSLSINPRFCDTDALGHINNTVLPVWFLEGRESILRI
                     FNPSLSTEEVSLVLVKLELEYLEEMFFGKKVEIRTYVLRIGTSSVLVGQEAWQDGKLC
                     ATGSATMVNFDKHSRKSVPIPADIRKKLSEHLLEQDR"
     gene            830..1096
                     /locus_tag="VFX11_15550"
     CDS             830..1096
                     /locus_tag="VFX11_15550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011070492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4940095.1"
                     /translation="MTASTAVPAVIHQPDKSRFVCDTGNGEAEMTYRLSGSTVDFNHT
                     YVPGSARGRGIAALLVEAGLAWARAQGLTIKASCSYVHAYLRQA"
     gene            1447..2913
                     /locus_tag="VFX11_15555"
     CDS             1447..2913
                     /locus_tag="VFX11_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HEX4940096.1"
                     /translation="MKRALKACLEAMQSAEDYVAWSDAAKEYDRLSGTEEWKEEDASP
                     HYDYKMIRLRFNELRTARERGDVNQLAFYINEGLHGNLGRIANPVLYEYSYFGTKKLI
                     TDYLTEVCASLRWIAEVELPNFSLVQKLEFFERTQQAFGQSALMLSGGAALGLFHLGV
                     LKSMWESGLLPNIISGSSAGSIMASVVATHTDEELKVMLDPEYIFVEAFRMTGWRSTL
                     RGKGLLDPAQLELALERNVKDLTFEEAYKRTGRRINITVSPADPHQESRLLNAVTSPH
                     VLIRKASLASCAIPYVYPPVMLWAKNINGEKVPYIPSRQWVDGSIKNDLPIQRLARIY
                     GVNHSIVSQTNPHVVPFLSRAESERTFASYVKDMLVNNVRYNINHGLDYAKNKITNQS
                     AALLVDKVHSMLSQKYAGDINIVPPRQPGNIFKILSNATPADVARFIRNGETSTWPKM
                     ELIRNSTCISRTINECLVRLKAEEERELVEYSSSAVRA"
     gene            3258..>3648
                     /locus_tag="VFX11_15560"
     CDS             3258..>3648
                     /locus_tag="VFX11_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX4940097.1"
                     /translation="MASRNTPLKQALQKKPDRPRVTPLDVFERARALWLQGERLNLGS
                     LAETLGVSRATLFRWVGNKELLMGEILWSTYQPLVERALDQATGKGVDFMVDVFRRVN
                     LDIMKSESLRQFMSQDPAYALRVLTSND"
BASE COUNT          762 a         1082 c         1031 g          773 t
ORIGIN      
        1 ggtgatcacg gtacagggat ggcagccgag gctggtgagg gtttcaatat cggcctggat
       61 gccggcgcca ccgctgggat catgaccggc taacaccagc acgatgggcg ggttggcaag
      121 gggcgggctg ggcggcatgg cgggtgtctc ctgacagtct ggatctgccc atcatagtcc
      181 agtgcaccgg gaatacccac tcccgccgtt gtgccggata atccctgccg aatccattat
      241 ttgcgcatcc gcgccgccgc cgcctggttg ccttgtgtcg ccaaaattgc cctggtggca
      301 gcaagtggtg tccctctgcg tgtataattc gcccgttcgg gtttctgatc tgttgaaagt
      361 ttgatcatgc ccccggtttc accagtcagt ggaataacaa tgatagcgtc ccatgtaatg
      421 ttctccctca gcatcaatcc ccgcttctgc gacaccgacg cactggggca catcaacaat
      481 accgtgctgc cggtctggtt cctggaagga cgcgaatcca tcctgcgcat cttcaatccc
      541 agcctgtcca ccgaagaagt cagcctggta ctggtgaagc tggaacttga atacctggaa
      601 gaaatgttct tcggcaagaa ggtggaaatc cgcacctacg tgctgcgcat tggcacttcg
      661 tcggtactgg tgggccagga agcctggcag gacggcaagc tctgcgccac cgggtctgcc
      721 accatggtga atttcgacaa gcattcccgc aaatccgtgc ccattcccgc tgacatccgc
      781 aagaagctgt cggaacatct gctggaacag gaccgctgag accccgccca tgaccgcctc
      841 cactgcagtg cccgccgtca ttcaccagcc ggacaaatcc cgcttcgtct gcgacaccgg
      901 caacggcgag gccgagatga cgtaccgcct gagcgggtcc accgtggatt tcaaccatac
      961 ctacgtaccg gggtccgccc gcggacgcgg cattgctgcg ctgctggtgg aggcgggcct
     1021 tgcctgggcc cgtgcacagg gcctgaccat caaggcatcc tgctcctacg tgcacgcgta
     1081 cctgcggcag gcttgaaccc gtcgcctggc gacttttcgt cccctatctt ctgccgcaac
     1141 cccttgtttg atcgattttt aatctgactg cgcgatcgtt ggactataat gtgactaatg
     1201 ggttttgtag acgtgacttt tgggtctgtc tatcaaccgg tctctggttt acaaaatgat
     1261 tcaattgctt catatattga tgatcggtgt ttcggcacac tcgggtgaat tgttggtcac
     1321 ccagccgggc acaagggaca ttcacgatac gcaccttcgt gacacgcatc gccaggaagc
     1381 gattgatcta ccaataacag ctgacattgc gcactctgac gaattgaagt ggtacagggg
     1441 gtaagaatga agcgagcgtt gaaggcctgt ctggaagcga tgcaaagcgc cgaggactat
     1501 gttgcctgga gtgatgccgc caaggaatac gatcgtcttt ccggcactga agagtggaaa
     1561 gaggaagacg cgtcaccgca ttacgactac aagatgatcc ggctgcgctt caatgaattg
     1621 cgcactgccc gggaacgtgg cgacgtcaac cagctggcgt tctacatcaa cgagggcctg
     1681 cacggtaacc tcggccgcat cgccaatcct gtcctctacg aatacagcta ttttggcacc
     1741 aagaagctca tcaccgatta cctcaccgaa gtctgcgcca gtctgcgctg gatcgcggaa
     1801 gtcgagttac ccaatttttc cctggtgcag aaactggagt ttttcgagcg cacccagcag
     1861 gcgttcggcc agtccgcgct gatgctcagc ggcggtgccg cactgggtct gtttcacctg
     1921 ggcgtactca aatccatgtg ggaatccggc ctgctgccga acatcatttc cggttccagc
     1981 gctggttcca tcatggcgtc ggtggtggcg acgcataccg acgaagaact gaaagtcatg
     2041 ctcgatcccg agtacatttt cgtggaagcc ttccgcatga ccggctggcg cagcaccctg
     2101 cgcggcaagg gccttctgga cccggcacag ctggagctgg cgctggaacg taatgtgaaa
     2161 gatctgacgt tcgaggaagc ctacaagcgc actggccgcc gcatcaatat caccgtgtcg
     2221 ccggcggacc cgcatcagga atcacgcctg ctcaacgcgg tgacctcacc ccacgtgctg
     2281 atccgcaagg catcgctggc atcctgcgcc attccctatg tgtacccgcc ggtgatgctg
     2341 tgggcgaaaa acatcaacgg cgagaaagtg ccctatattc cgtcgcggca atgggtggac
     2401 ggttccatca agaacgatct gccgatccag cgcctggcgc ggatttacgg cgtaaaccac
     2461 tccatcgtca gccagaccaa tccgcacgtg gtgccgttcc tgtcccgcgc cgaaagcgag
     2521 cgcacgtttg cctcgtatgt gaaagacatg ctggtgaaca atgtgcgcta caacatcaat
     2581 cacgggctgg attacgccaa gaacaagata accaaccaga gcgctgctct gctggtggac
     2641 aaggtgcatt ccatgctgtc gcagaaatat gcgggcgaca tcaatatcgt gccgccgcgc
     2701 cagccgggca atatcttcaa gattttgtcc aatgccacgc cggcggatgt ggcacgcttc
     2761 atccgcaacg gtgagaccag cacctggccg aaaatggaac tcatccgtaa ttccacctgc
     2821 atcagtcgca ccatcaatga gtgcctggtg cggttgaagg cggaagaaga acgcgaactg
     2881 gtggaatact ccagcagtgc cgtgcgcgcc tgatccgtcc ccgcacactg ccccgcgatg
     2941 tcgcagccgg ttgctccggt tgcggcatgg gcgcggcaag ccagccgatt tccagcatcg
     3001 attttccatc acctgattcg cagcaatcgc taactcccct acattaaaaa ggcgatttca
     3061 gcaggatttg ctttcatttc ggccgcaaag gtccgcggca aattttttct gcgtaaattg
     3121 tcctgacatg cgtgcagtcc ggatgtattc tgcgccctca aggatagccg tttgttggac
     3181 ggtactttgc cagcgtcgcg cctgcctgtc tgttgaccgg cgcgatccgg caaaccgcag
     3241 taaaggagtc agtcaccttg gccagccgta atacccctct gaaacaggct ctgcagaaaa
     3301 agccggatag accgcgcgtg acgccgctgg atgtgttcga gcgtgcgcgc gcactgtggc
     3361 tgcagggcga gcgactcaat ctgggatcac tggcggaaac ccttggggtc agccgcgcca
     3421 cactgttccg ctgggtgggc aacaaggaat tgctgatggg cgaaattctc tggtccacct
     3481 accaaccgct ggtggagcgc gcgctggatc aggccaccgg caagggcgtc gatttcatgg
     3541 tggatgtgtt caggcgcgtc aatctcgaca tcatgaaatc cgaatccctg cggcagttca
     3601 tgtcgcagga cccggcttat gcattgcggg tgctcacgtc caatgatt
//
LOCUS       DASUEG010000826         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2065268, whole genome shotgun sequence.
ACCESSION   DASUEG010000826 DASUEG010000000
VERSION     DASUEG010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065268"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFX11_15565"
     CDS             <1..295
                     /locus_tag="VFX11_15565"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4940098.1"
                     /translation="TVNDDSGEYIQKVIACETVDVSERGLKIYMSEEVAHGTITDLCV
                     VLAGQEGRFFLKAEVKWISTSGDDGWYFAGFEIYDAENTDYDKWLVMVRERKG"
     gene            complement(330..2120)
                     /locus_tag="VFX11_15570"
     CDS             complement(330..2120)
                     /locus_tag="VFX11_15570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940099.1"
                     /translation="MLPDALASETRANLRQAWVSLTEDAPRNDLSLNLTYLEDADHTL
                     TVDDARRQVEAGAFQGSQTTTLNFGFTRSAYWFHLHLDNRDSAQEEWVLEFQYPIIDQ
                     IDAWYFFADGRQEHRQAGDSIPFHQRSKSNHFTNFELKLPRGDQVDILLRVETSGAIQ
                     MPTVIWNEDAYAAHYHKVQIVFGLYYGLIFAMAVYNGLLWVSLRDRTYFLCVAYIAGY
                     GVFQLALNGLAFEYLWPESPWWNNRSIAFLMSFGLFFIIAFSSAFLVLPKNAPRASRL
                     FNFMMLYFVAMMAGALFVQYQTLIRLATLGSMLCAVAIFVVGFYCWITRFKPARYFMM
                     SWAALLGGMLAYTLKTFGLLPSNFFTEYAMQIGSAMEMLLLSFALADRIKIVTEENSR
                     IQREAKTVLEANVKARTQELEQANRKLAELSATDGLTGLKNRRYFDEAFALECKRSTR
                     YLEHLAVLLIDIDHFKNINDTYGHPVGDQVLAQIAEIMRKNVRRDTDTVARYGGEEFV
                     IILPSTDTGGALQVAEKILQAVRRHLFVVGELALPLTVSIGVASLTLPDSQSVHNILE
                     QADIALYQSKQNGRDRCTLYREQHGKQAAS"
     gene            2547..>2805
                     /locus_tag="VFX11_15575"
     CDS             2547..>2805
                     /locus_tag="VFX11_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4940100.1"
                     /translation="MQFQGTERYVATDDLKLAVNAAITLQRPLLIKGEPGTGKTMLAE
                     EVAAGLGLRLIQWHIKSTTKAHQGLYEYDAVSRLRDSQLGDN"
BASE COUNT          610 a          792 c          844 g          559 t
ORIGIN      
        1 caccgtgaat gacgacagcg gtgaatacat ccagaaagtc attgcctgcg aaacggtgga
       61 tgtgtccgag cgcgggttga aaatctacat gagcgaggaa gtggctcacg gcaccatcac
      121 ggatctgtgc gtggtcctgg cggggcagga gggccgtttt tttctcaagg ctgaagtgaa
      181 gtggatatcc accagcggtg atgatggctg gtatttcgcg ggctttgaaa tctacgatgc
      241 ggaaaacacc gattatgaca agtggctggt catggtgcgg gagcgcaagg gctgaattgc
      301 aggggctgaa ttaaatgggc cgtgcgccct caggacgctg cctgcttgcc gtgctgttcc
      361 cggtacagcg tgcagcggtc gcggccgttc tgcttggact ggtaaagcgc aatgtcggcc
      421 tgttccagaa tgttgtggac actctgtgag tccggcaggg tcagggaggc aaccccgatg
      481 ctcacggtca gcggcagcgc gagttccccc accacaaaca gatgtcgtct gacggcctgc
      541 aaaatctttt ccgctacctg cagggcgccg ccggtgtccg tggatggcag gatgatcacg
      601 aattcctcgc cgccatagcg ggccactgta tccgtatccc gccgcacgtt tttacgcatg
      661 atctccgcaa tctgcgccag aacctgatcc cccaccggat gaccataggt atcgttgatg
      721 ttcttgaaat ggtcgatgtc gatcaacagc accgccaggt gttccagata gcgggtggaa
      781 cgtttgcatt ccagggcgaa ggcttcatcg aagtagcgcc ggtttttcag gccggtcagc
      841 ccgtccgtcg cgctcagttc cgccagcttg cggttggcct gttccagttc ctgggtgcgg
      901 gctttcacat tggcttccag tacggtcttc gcttcacgct ggatgcggga attttcctcg
      961 gtcacaatct tgatgcggtc agccagggcg aatgacagca gcagcatttc catggcagac
     1021 ccgatctgca tggcgtactc ggtgaaaaaa ttgctgggca acaggccaaa ggttttcagt
     1081 gtataggcca gcatgccacc cagcagcgct gcccaggaca tcatgaaata gcgtgccggc
     1141 ttgaaccggg tgatccagca ataaaatccc accacaaata tggccacggc acagagcatg
     1201 gaacccagtg ttgccagccg gatgagggtt tggtactgca caaacagcgc acccgccatc
     1261 attgccacga aatacagcat catgaagttg aacagcctgg aggcgcgcgg tgcatttttc
     1321 ggcaatacca gaaaggcgct ggaaaacgcg atgatgaaga acagcccgaa cgacatcagg
     1381 aacgcaatcg agcggttgtt ccaccagggc gattccggcc acaggtattc aaatgccagc
     1441 ccgttcagtg ccagctggaa cacgccgtag ccggcgatgt aggccacgca cagaaaatag
     1501 gtacggtccc gcagcgaaac ccacagcagg ccgttataga ccgccatggc gaagatcagg
     1561 ccgtaataca gcccaaacac gatctgcact ttgtggtagt gggcggcgta cgcgtcctcg
     1621 ttccagatca ccgtcggcat ctggatggcg ccgctggttt ccacccgcag caggatatcc
     1681 acctgatcgc cccggggcag cttcagttcg aagttggtga aatgattgct cttggagcgc
     1741 tggtgaaacg gaatggaatc gccggcctgg cgatgttcct ggcggccgtc ggcaaaaaag
     1801 taccaggcat cgatctggtc gatgatgggg tactggaatt ccagcaccca ttcttcctgg
     1861 gcggaatcgc ggttgtccag gtgcaggtga aaccagtagg cggaccgggt aaagccaaaa
     1921 ttcagtgtgg tggtctggct gccctggaag gcaccggctt ccacctggcg acgcgcatcg
     1981 tccaccgtca gcgtgtgatc ggcatcttcc agataagtga ggttgaggga cagatcgtta
     2041 cgcggcgcat cttccgtcag cgacacccat gcctggcgca gattggcccg ggtctcggag
     2101 gccagggcgt cgggcaacac cagcaggctc cacaggccaa caacggcgag caggagaagg
     2161 cgggttctgt agcggacggt cggaacactc aacacttaca ggccttgaaa tgacagcatt
     2221 ttccggggat ttatcgtttt aagtgtatcc ggtaatgtgg attatgcgag tcaccgcact
     2281 ggccggtcct gacatcctga tccggcgcta accgaccccg gttcagctgg ggggcaagct
     2341 ggtgttctgc ctcggaataa gcggcaactg tgccctcggc agaggggagg agatttgatc
     2401 atttttgcca gtgatctggc gtctgcggcc atttgcagtt atggcgccgg tctttaaagt
     2461 agcacgattg tccccgacac ccgcgctgga acagggccgg tgtacgatat actcccgcac
     2521 ctacacggca gatcaaggca gaacccatgc aattccaagg caccgaacgc tacgtcgcca
     2581 ccgacgacct gaaactggcg gtgaacgccg ccatcaccct gcaacgtccc ctgctgatca
     2641 agggcgaacc cggtaccggc aagaccatgc tggccgagga agtcgccgcc ggcctgggtc
     2701 tgcgcctgat ccagtggcat atcaagtcca cgaccaaggc ccatcaaggc ctgtatgaat
     2761 acgacgccgt gtcgcggctg cgggattccc agctgggcga taacc
//
LOCUS       DASUEG010000827         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1225752, whole genome shotgun sequence.
ACCESSION   DASUEG010000827 DASUEG010000000
VERSION     DASUEG010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225752"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2421
                     /locus_tag="VFX11_15580"
     CDS             <1..2421
                     /locus_tag="VFX11_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940101.1"
                     /translation="HAGNGSPYYVFIADRRRQRIFCSNLASPSTLLKHESLEADLYQA
                     YLHSPATSLLQFRSIKEDLLDVINKADESCESHLFRFKDESGYWRFGWVSSASMRGGV
                     AGDQDYLCGMISLAQNSAVSTPEQQIGSRLVWMTDRNLRLTHVCQECRTLGFDAASLL
                     ENGELGFLPQESREFFESLAFGEFGLGDSDADSCSMPDTRVVRQPLCSVDGTSISATI
                     QFSLLFDENGAVTGALGIADFMPSRELLESERQLFSAIFESISEGIFVTDRNGYILRA
                     NPAFYDVTGYAPQQILGVHCGHLWKHSHGPELFRRIRQALTRDRSWREETVYTRANGE
                     TRPALVSFNQTHNLKHEVQHYVGVLMDIADKKQNERKIHRLAFYDSLTEVPNRRLFNE
                     RLEVAVADAETGGTVLAVLFLDLDRFKPVNDSLGHSAGDQLLKGVARRLLYCLGEGDT
                     VARMGGDEFAIILRDLPDELAEQTAIKMASRVLAQFFSPFMIASREVFTSCSIGIALY
                     PHHGRSAEVLLRSADTAMYAAKRAGKNNYQFFDEIMNKRAMDRLIMENAMRKALVNED
                     FELYFQPQYSVPNGVMTGVEVLLRWDHPQFGDVKPVEFINLAERTDLIIPLGEWVFQQ
                     VCEKMSLWYQQGIRLGRVSVNVSANQFKRKDFAEWVLFQVQRYGVDPAQLEIEITESA
                     LMEDVEHSLTVLTALRRAHIRIAIDDFGTGYSSLSYLRRFPISTLKIDKVFIDDIAVD
                     RGTLELTQTVIAIAKSLKLGVIAEGVETWDQYRLLSQHGCDEVQGFYMSRALPEAALL
                     ELVACEQG"
     gene            complement(2444..3199)
                     /locus_tag="VFX11_15585"
     CDS             complement(2444..3199)
                     /locus_tag="VFX11_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940102.1"
                     /translation="MGRLNGKIALVTGAGQGVGQGIAFALAAEGARVAVTGRTLQKLE
                     STCAEIQRRGGEALPVQCNVKEPESLRACVDTVVTHFGGLNILVNNAQEVPLGALLDV
                     TDAAFNAGWESGPLATFRLMKLCHPYLKGDGCIINVGSSAAKRWDMSRYGAYAAVKEA
                     IRSLTRAAACEWGADNIRCNAILPHAKSPGLEGWMQQNPAEAAAFVKTIPQRRVGDCE
                     QDIGRFVALLCCPESSYINGQSIGVDGGQALIG"
     gene            complement(3227..3817)
                     /locus_tag="VFX11_15590"
     CDS             complement(3227..3817)
                     /locus_tag="VFX11_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020581499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4940103.1"
                     /translation="MSNVTNNKNNLSSDLTAQLIDLLDKQAIQELVHAYCNAADRHDY
                     AKMRSLYHPDARDDHGGFFQGLAMDFIDQLPAIQEPMEILHHNVTTLNIKLDAADAAV
                     RKAEGEVYVLAFHKVKTPEGSFDLLIGGRYFDQYEKRDGLWKFSHRAVVADWVNVHNP
                     SIVDLQHPVIAGSRIGRPGQDDPSYAFFSLLQWGQR"
BASE COUNT          775 a         1218 c         1246 g          844 t
ORIGIN      
        1 cacgcgggca acggatcacc gtattatgtg tttatcgccg accggcgtcg gcaacgtatt
       61 ttttgttcca accttgcctc tccctccacc ctgctcaaac acgaaagtct cgaagccgat
      121 ctgtatcagg cctatctgca cagtcccgcc acgtccctgc tgcaattccg cagcatcaag
      181 gaggatctgc tggatgtgat caacaaggcc gacgaatcct gcgaaagcca cctgttccgt
      241 ttcaaggacg aaagtggcta ctggcgtttc ggctgggtca gcagcgcgtc gatgcgcggt
      301 ggcgtggcgg gggaccagga ttatctgtgc ggcatgatct cgctggcgca gaacagtgcg
      361 gtcagcacgc cggagcagca gatcggttcg cgtctggtgt ggatgaccga ccgtaacctg
      421 cgcctcaccc atgtctgcca ggaatgccgc acactgggtt tcgatgcggc ttcgctgctg
      481 gaaaacggcg agctgggatt tttgccgcag gaatcccgcg aattttttga atcgctggcg
      541 ttcggcgaat ttggcctggg cgatagcgac gccgacagtt gcagcatgcc ggacacccgg
      601 gtggtgcggc agccgctgtg ttcggtggat ggtacctcga tctccgccac catccagttc
      661 tcgctgctgt tcgacgaaaa cggcgcagtg acgggcgcgc tgggtatcgc cgatttcatg
      721 cccagccgcg aactgctgga aagcgaacgg caattatttt ccgcgatttt cgaatcgatt
      781 tccgaaggca ttttcgtcac cgaccgcaat ggctatatcc tgcgggcaaa ccccgcattc
      841 tatgacgtca ccggctatgc accgcagcag attctcggcg ttcactgcgg ccacctgtgg
      901 aaacattccc acggcccgga actgttccgt cgcatccgtc aggcgctcac ccgcgatcgc
      961 agctggcgcg aagagacggt ttatacgcgt gccaatggcg aaacccggcc ggccctcgtc
     1021 agtttcaacc agacccacaa tctcaagcat gaagtgcagc actatgtggg tgtgctgatg
     1081 gatattgccg acaagaaaca gaacgaacgc aagatccacc gcctggcatt ttacgattcc
     1141 ctcactgagg tacccaaccg ccggctgttc aacgagcggc tggaagtggc ggtggccgac
     1201 gcggaaaccg gtggcacggt gctggcggtg ctgtttctgg atctggaccg gttcaagccg
     1261 gtgaacgatt ccctcggcca cagcgcgggc gaccagttgc tgaaaggcgt ggcgcggcgg
     1321 ttgctgtatt gtctggggga gggcgacaca gtggcgcgca tgggcggcga cgagttcgcc
     1381 atcattctgc gggatctgcc ggacgaactg gcggagcaga cggcgataaa aatggcatcg
     1441 cgggtgctgg cgcagttttt ctcgccgttc atgatcgcca gtcgagaagt attcacgtcc
     1501 tgcagcattg gcatcgcact gtatccccat catggccgtt cggcagaagt gctgctgcgt
     1561 agcgccgaca ccgcgatgta tgccgccaag cgtgccggca agaacaatta ccagttcttc
     1621 gacgagatca tgaacaagcg ggccatggac cgtctgatca tggaaaatgc catgcgcaag
     1681 gcgctggtga atgaggattt cgaactctat ttccagcccc agtacagcgt gcccaacggc
     1741 gtgatgaccg gcgtggaagt gctgctgcgc tgggatcatc cgcaatttgg cgacgtgaag
     1801 ccggtggaat tcatcaatct ggctgagcgc accgacctga tcattccgct gggtgaatgg
     1861 gtgttccagc aggtgtgcga gaaaatgtcg ctctggtacc agcagggcat ccggctgggg
     1921 cgggtgagcg tgaacgtgtc cgccaaccag ttcaagcgca aggatttcgc cgagtgggtg
     1981 ctgttccagg tgcagcgcta tggcgtggac ccggcgcagc tggaaatcga gatcaccgaa
     2041 agcgcgctga tggaagatgt ggagcacagc ctgaccgtgc tgacggcgtt gcggcgggcg
     2101 catatccgca ttgccatcga cgatttcggc accggttatt catcgctgag ttatctgcgc
     2161 cggttcccca tcagcacgtt gaaaatcgac aaggtgttca tcgacgatat cgcggtggat
     2221 cgcggtactc tggaactgac ccagaccgtg attgcgattg caaaaagcct gaagctgggc
     2281 gtgattgcgg aaggggtgga aacctgggac cagtatcggt tgctgagcca gcatggctgc
     2341 gacgaggtgc agggtttcta catgagccgc gcgctgcccg aagccgcgtt gctggaactg
     2401 gtagcctgcg agcagggttg atttttcctg cccgcgcagt cgttcagccg atcagcgcct
     2461 gccctccatc caccccgatg ctttgcccgt tgatatagga cgattccggg caacagagca
     2521 gcgccacaaa ccgcccgata tcctgttcgc aatctcctac ccgccgttgc ggaatcgttt
     2581 ttacgaatgc tgccgcttct gccggattct gttgcatcca gccttccagc cccggcgatt
     2641 ttgcatgggg cagaatggcg ttacagcgga tattgtccgc gccccattcg caggcggcag
     2701 cgcgggtgag ggaacggatg gcttctttca ccgccgcgta ggcgccgtag cggctcatgt
     2761 cccagcgttt ggcggcagag gaaccgacgt tgatgatgca gccatcgcct ttcagatagg
     2821 gatgacagag tttcatcagg cggaaggtgg ccagcgggcc ggattcccag ccggcgttga
     2881 aggcagcatc ggtaacgtcc agcagagcgc ccagcggcac ttcctgcgca ttgttcacca
     2941 gaatgttcag cccgccgaag tgcgtcacca ccgtgtcgac gcaggcgcgc aacgattccg
     3001 gttctttcac gttgcactgt accggcagcg cctcgccacc ccggcgctgg atttcggcgc
     3061 aggtgctttc cagtttctgc agcgtgcgcc cggtcaccgc gacgcgcgcg ccttcggctg
     3121 ccagtgcaaa cgcgatgccc tgacccacac cctggccggc gccggtcacc agcgcgattt
     3181 tgccgttcag tctgcccatg ttgttcgctc cccctctata aaataatcag cgctggcccc
     3241 actgcaacag cgagaaaaag gcataggacg gatcgtcctg ccccggccgg ccaatgcgcg
     3301 atcccgcaat caccggatgc tgcagatcca cgatcgacgg gttgtgcaca ttgacccagt
     3361 ccgccaccac tgcgcggtgg gaaaatttcc acaggccgtc gcgcttttcg tattggtcga
     3421 agtagcgtcc gccgatcagc agatcgaaac tgccctcggg tgttttcacc ttgtggaacg
     3481 ccagcacata cacttcgccc tcggccttgc gcacggctgc gtcggcagca tccagtttga
     3541 tgttcagggt ggtgacgttg tgatgcagga tttccatcgg ttcctggatc gccggcagct
     3601 ggtcgatgaa atccatggcc aggccctgga agaaaccgcc gtggtcgtcg cgggcgtccg
     3661 ggtgatacag gctgcgcatc ttggcgtaat cgtggcggtc ggcggcgttg cagtaggcgt
     3721 ggaccagttc ctggatggcc tgcttgtcca gcaggtcaat cagctgcgcg gtgaggtcgc
     3781 tggagaggtt gttcttgttg ttggtgacat tgctcatcta accgtgcgct ccgtcaaagg
     3841 gttgtcccag cctgcagact aagatgcacc gggtggggga acaatcgtcc gaactgacta
     3901 ttgttcccgt ccggttgatg gccccgcccg gttgccatga aaagcccgac tgattggagt
     3961 agcatcctgc ccatcgcccc ctgttttatt gctgtgacgg aagccccatg acgccgcaaa
     4021 agcctgaccc cgtgcccggc cgcaacccgc cgggcaagcc cactcccggc aagcctgtcc
     4081 ccg
//
LOCUS       DASUEG010000828         8751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_671368, whole genome shotgun sequence.
ACCESSION   DASUEG010000828 DASUEG010000000
VERSION     DASUEG010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671368"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFX11_15595"
     CDS             <1..301
                     /locus_tag="VFX11_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004724279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX4940104.1"
                     /translation="SSEQSIPEPLAKLQLGRSYIFNLRNVTPHTHPIHLHGNTWTVLE
                     SNQRKITPYHTDTVLVRRNEKVKVAFVADNPGRWMYHCHVIEHMKTGLMGYISVA"
     gene            complement(283..1410)
                     /locus_tag="VFX11_15600"
     CDS             complement(283..1410)
                     /locus_tag="VFX11_15600"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEX4940105.1"
                     /translation="MQRRRFLQGMLGTLAATGLPVSTLVPLMLGGCQREPLLPGTLLS
                     ACDDARGNHFVASIQPDGSLGFQIPVPLRAHDSCASRERDRALFFARRPGHQIYVVDL
                     HERTLIQTLDAEPGRHFYGHGVISADGQWLFTTEQIYATGQGMIGVYRMTHPIRRETE
                     FPSHGMDPHQLQWLSDGETLVIANGGILTHPDQNRENLNPDTMQPALTYVRVRDGALL
                     ESRQPPHHQMSLHHLDVSPQDQVVVGVQHQGALTDEVALVGSHRRGSDMQWWRADELT
                     QLRMQQYTASVAVNSNGRQAVVSCPRGNGITVWDMQSGQLIRMLDITDAAGVRRQPQQ
                     AGWVGTNGAGALVRIDSGAATVALQTLRQYPLRWDNHATLI"
     gene            complement(1410..2483)
                     /locus_tag="VFX11_15605"
     CDS             complement(1410..2483)
                     /locus_tag="VFX11_15605"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX4940106.1"
                     /translation="MRSLLLLSIALLSLTACKDDMTPEQRWLATTTEQIQRGYQQLSA
                     QTRAQQQAADAFCQQPDAPGLQTMQAAWRESMGAWQHIQWLRIGPIAQDNDDWKLQFW
                     PDKKNILQRKMQDILDGNEPVSVDSLAHASVVVQGFSAQELLLFDPQFATVEKFAGRQ
                     CDLLRASTTLTARVGQKLADAWQDETWLERWVTPVATQAGLAPAQIRNGEILDALLAQ
                     IERIKNDKLGEPMGLKTRDKKPNGYFAESWRSENSLANLQNNLLAVQQMVRPKQGYGL
                     FQYLKDSQHQAVADELVIKLDNAQQALQQIKPPLTSAVTDAAQQAALQNAHKALGDLA
                     TFCKQKLAPALGLTLGFNSNDGD"
     gene            complement(2553..3929)
                     /locus_tag="VFX11_15610"
     CDS             complement(2553..3929)
                     /locus_tag="VFX11_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX4940107.1"
                     /translation="MLLVLPAIFPCAFADNHYPVKSGGDTTVRKADVNAFSLPSHNMS
                     FERKVDFSVGNSFFRNPWVIAPSSTAARDGLGPLFNTNGCQNCHIKDGRGHLPAEGDD
                     NAVSVLLRISVAPRTPEQWQRVYRDGGIPDPHYGTQIQDFAVPGLEPEARLDIRYETR
                     VVTLADGTSVRLRKPVVRLNQLAYGPLHPDLQMSLRLAPPMIGLGLLEAIPEAVLQSF
                     ADEQDRDGDGISGRVNRVWNVESAATAVGRFGWKAEQPSLKQQNAAAFAGDLGVTSGL
                     FLRTDCTHAQTACKGIPTGGVPEASDHVLDLVTFYSRNLAVPARRNADAPLVQRGGQL
                     FAAAGCESCHRTGIVTAELPAQPEQSLQTIHPYTDLLLHDMGEELSDGRPVFRADARE
                     WRTAPLWGIGLTATVSGAEHYLHDGRAHSLLEAILWHGGEAEHSREQVRNMSAADRDA
                     LIAFLQSL"
     gene            complement(3980..5236)
                     /locus_tag="VFX11_15615"
     CDS             complement(3980..5236)
                     /locus_tag="VFX11_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX4940108.1"
                     /translation="MKPILHALVGLGLLTAVPVVAATPEQDVVAQYANVAHAVFADAL
                     QSAKTLQGSVEALLSAPSDANLQKARTAWKAARVPYQQSEVFRFGNTNVDEWEGQLNA
                     WPLDEGLIDYVATGKYHHELGNVGANANIIANTSLKLGSDTLDLANITPELLASLNEL
                     GGSEANVATGYHAIEFLLWGQDLNGSGPGAGNRAYTDYVQGAGCTNGHCDRRAAYLKA
                     ATQLLVSDLANMEQQWAPGKDNYRAELLKGKPDAGLVKMLYGMGSLALGELAGERLKV
                     ALEANSPEDEHDCFSDNTHHSHFYDALGIENIYLGRYQRIDGSVVKGASLSDLVAAKS
                     PDTDKAVKAQFSKTMSAMQKLVDSAESTTSPMKFDQMIAENNPAGQALVKDAIAQLVA
                     LTRQIETAAKTIGIDELNPDTAEHEF"
     gene            complement(5272..6381)
                     /locus_tag="VFX11_15620"
     CDS             complement(5272..6381)
                     /locus_tag="VFX11_15620"
                     /inference="COORDINATES: protein motif:HMM:NF033652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbtU family siderophore porin"
                     /protein_id="HEX4940109.1"
                     /translation="MSKLHPPVAQALSALLLALSATAYGAEPQSQAQPTVEERLTALE
                     QQSAKPAALPDSVQVRGLVEVEAVGGEDYAGQSYSDLTVATVEVGVDAAINDKVSAAL
                     VFLYEQDQTDFGVDEATLTIAELVGPVNFTVGKMYVPFGTFETALINDTRVLELAETN
                     KTAALFAFAQSGIEAGAYVFDGDQERERHAENYGVTVGFVQETFQVGVDYLSSITESD
                     GVADLNETLSAEADPVIDPAFEWDDAAPAYNVHGQIALGAVVLIAEYLGLADPLVDAT
                     NGIEIEPAAAQLELDFTATLAGHEYTLAAGYQQTEDALVLGLPEERMSLGCSTEIYRN
                     VTLGGEIWRDTDYGLADGGSDEESDNLVVQLAASF"
     gene            complement(6461..7393)
                     /locus_tag="VFX11_15625"
     CDS             complement(6461..7393)
                     /locus_tag="VFX11_15625"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4940110.1"
                     /translation="MRSSSGRLSVFLLDLMLKSPFRPKLAVADGELLRSRKSMDRWGE
                     KMPPVRGVTVTNETLAGLYCEIHTPGDAHSNQVLLYFHGGGFCLGSPRSHRNLVSRLA
                     QAAGIRAVVPDYRKSPEHPFPAALDDTVRLYRYFLDQGIKPANIVFAGDSAGGNLVLT
                     TLLKLKQEGLPLPAAGCPISPWCDVSLGGETIQRNAAKDFILTPLLLQQFGERYVGQE
                     RWNDPLVSPVQGDFAGLPPLLIQVGSREILLDDARRVAQRAAQAGVPVQLEVWDDMQH
                     VWHYTAFLLKDGRRALRRMGEFLKANIGMRKTGS"
     gene            complement(7523..8446)
                     /locus_tag="VFX11_15630"
     CDS             complement(7523..8446)
                     /locus_tag="VFX11_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX4940111.1"
                     /translation="MTTAYITHRDCELHDMGGNHPEAPARLRAIRDYLMERQIWDLLL
                     QVDAPKIEKKHLYRVHSRDYVDAIFEQFPLRDLRDLGPDVVANPHTLDAALRAAGGVC
                     KAVDLVMKGEAGNAFCAVRPPGHHAEMRRAMGFCVFSNVAVGAAYALQTYNLDRVAII
                     DIDVHHGNGTEDILQYDERVLFCSSFQYPCYPYQVPDNSRSNIIHTPLHAGTSSDEYR
                     DKVRNEWLNKLETFHPQLIMMSTGFDAHQADPLADVHLDERDYRWITEELMLVADRCC
                     NGRIVSSLEGGYNLTALSRSAYQHIRTLMRI"
     gene            complement(8469..>8751)
                     /locus_tag="VFX11_15635"
     CDS             complement(8469..>8751)
                     /locus_tag="VFX11_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4940112.1"
                     /translation="GKWIEIGISRYVQNPDGASCEFAVVVADQWQRTGIGYKLMELLI
                     DAARQKGLKYMDGIVLKENTGMRKLARAMGFELRDHDQDEDVVYVIKKL"
BASE COUNT         1653 a         2723 c         2614 g         1761 t
ORIGIN      
        1 ctcatccgag caaagcattc ccgaaccact ggcgaaattg cagctgggcc gctcctacat
       61 tttcaacctg cgcaatgtaa cgccgcatac ccatccgatt cacctgcatg gcaatacctg
      121 gacagtgctg gaatccaacc agcgcaagat cacgccctac cacaccgaca cggtactggt
      181 gcgacgcaat gaaaaagtga aggtggcttt tgtggcggac aatccggggc gctggatgta
      241 ccactgccac gtgatcgagc acatgaaaac ggggttgatg ggttatatca gcgtggcgtg
      301 attgtcccag cgcagcgggt attgccgcag ggtttgcagg gcaacggtgg cagcgccgga
      361 atcgatccgc accagcgcgc ccgctccgtt ggtgccgacc cagccggcct gttgcggctg
      421 acggcggaca cccgcagcgt cggtaatatc cagcatccgg atcagctgcc cggactgcat
      481 atcccacacc gtaatgccgt tgccgcgcgg acaactcacc accgcctgcc tgccatttga
      541 attcaccgcg acgctggccg tgtactgctg catgcgcagt tgcgtgagtt cgtctgccct
      601 ccaccactgc atgtcgctgc cgcgacggtg actgcctacc agcgccacct catcggtaag
      661 tgcgccctga tgctgcacgc ccaccaccac ctgatcctgc ggcgacacat ccagatgatg
      721 cagactcatc tggtgatggg gcggctgacg ggattccagc agcgcgccgt cgcgcacgcg
      781 cacgtaggtc agcgccggct gcatggtgtc cggattgaga ttttcccggt tctggtccgg
      841 atgggtgagg atgccgccgt tggcaatcac cagcgtctcg ccgtcgctga gccactgcag
      901 ctggtgcggg tccatgccat gggagggaaa ttcggtttcg cgccggatcg gatgcgtcat
      961 gcggtacacg ccgatcatgc cctgcccggt tgcgtaaatc tgttcggtgg tgaacagcca
     1021 ctggccgtcg gcgctgatga caccgtggcc gtaaaaatgg cggccaggtt cggcatcaag
     1081 ggtctggatc agggtgcgtt cgtgcagatc caccacgtaa atctggtggc cgggacggcg
     1141 cgcgaaaaac agggcgcgat cgcgttccct tgacgcacaa ctgtcatggg cccgcagcgg
     1201 caccggaatc tggaaaccga gcgaaccatc gggctgaatg ctggcgacga aatgattacc
     1261 ccgggcatca tcgcaggccg acagcagcgt gcctggcagc aacggctcgc gctgacagcc
     1321 acccagcatc agcggcacca gcgtcgacac cggcaaaccc gtcgccgcca gtgttcccaa
     1381 catgccctgc aggaaacgtc gccgctgcat cagtcaccgt cgttggaatt gaaacccagc
     1441 gtcagtccca gcgccggcgc cagcttctgc ttgcagaacg tggccagatc ccccagcgcc
     1501 ttgtgtgcgt tctgtagtgc ggcctgctgc gcggcgtcgg ttacggcaga tgtcagcggt
     1561 ggcttgatct gctgcaacgc ctgctgtgcg ttgtccagct tgatgacaag ttcgtccgcc
     1621 acggcctgat gctggctgtc cttcaggtac tggaacaggc cataaccctg tttgggccgg
     1681 accatttgct gcaccgccag caggttgttc tgcagattgg cgagagaatt ttcgctgcgc
     1741 caggattcgg cgaaatagcc gttgggcttt ttatcgcggg ttttcaggcc catgggttcg
     1801 cccagcttgt cgttcttgat gcgctcgatc tgggccagca gcgcgtccag gatttcaccg
     1861 ttgcggatct gcgccggagc gaggcccgcc tgcgttgcca ccggggtaac ccagcgttcc
     1921 agccaggttt catcctgcca ggcgtccgcc agtttttgcc cgacccgggc ggtcagcgtg
     1981 gtgctggccc gcagcaggtc gcactgacgg ccagcgaact tttccaccgt cgcaaactgc
     2041 gggtcgaaca acaaaagttc ctgcgcgctg aaaccctgca cgaccacact ggcatgagcc
     2101 aggctgtcga cactgacggg ttcattgcca tccagaatgt cctgcatttt tcgttgcagg
     2161 atgtttttct tgtcgggcca gaactgcagt ttccagtcgt cgttgtcctg cgcgatcggt
     2221 cctatgcgca accactggat gtgttgccag gcccccatgg attcgcgcca ggctgcctgc
     2281 atcgtctgca gacctggcgc atccggttgc tgacagaaag catctgccgc ctgctgctgc
     2341 gcacgcgtct gcgctgacag ttgctggtag ccacgctgga tctgttcagt ggtggtcgcc
     2401 agccagcgct gctcgggcgt catgtcgtcc ttgcaggcgg tcagcgacag caaggcgata
     2461 ctcagcagta acagtgaccg catttttctt cgcacgaatc tgctttttcg caccaatctg
     2521 ggttgcatgt cggttccgga atagcaaaaa aattacaggg attgcaggaa ggcgatcagg
     2581 gcgtcgcgat ccgccgcgct catgtttcta acctgttcac ggctgtgttc ggcttcgccg
     2641 ccgtgccaga ggatggcttc cagcagggaa tgggcacggc cgtcatgcag gtaatgttct
     2701 gcgccactca ccgtagcggt caagccgatt ccccatagcg gggctgtacg ccactcgcgg
     2761 gcatcggcgc gaaagaccgg gcgtccatct gaaagttcct cgcccatatc gtgcagcagc
     2821 agatcggtgt agggatgaat ggtctgcaac gactgctccg gctgcgccgg caactccgca
     2881 gtgacgatgc cggtacggtg acaactttcg caaccggccg cagcaaacag ctggccaccc
     2941 ctctgcacca ggggagcatc cgcattgcgg cgcgcgggca ccgccagatt gcgggaatag
     3001 aacgtcacca gatccagcac atgatcgctg gcttccggca caccgcccgt gggaatgcct
     3061 ttgcaggcgg tctgcgcatg ggtgcagtcg gtgcgcagaa acagtccgga ggtaacgcca
     3121 agatcgccgg caaacgccgc cgcattctgc tgcttcaggc tgggctgctc tgccttccag
     3181 ccaaaacggc cgaccgcagt ggcagccgat tccacgttcc acacccggtt cacccggccg
     3241 gagatgccgt cgccatcccg atcctgctcg tcggcgaatg actgcaacac agcttccgga
     3301 atcgcctcca gcagacccag gccgatcatc ggcggcgcca gccgcaacga catttgcaga
     3361 tcgggatgca ggggcccgta cgccagctgg ttcagtcgca cgaccggttt gcgcagacgc
     3421 acgctggtgc catccgccag tgtgacaacg cgggtttcgt agcggatatc cagcctggct
     3481 tccggttcca ggcctggcac cgcgaaatcc tggatctggg tgccgtaatg cggatcggga
     3541 atgccgccgt cgcggtaaac ccgttgccat tgttccggtg tgcgcggagc gacgctgatg
     3601 cgcagcagca ccgacacggc gttgtcgtca ccctccgccg gcagatgccc gcgaccgtcc
     3661 ttgatgtggc agttctgaca gccattggtg ttgaacagcg gccccaggcc atcccgcgcg
     3721 gccgtggacg acggcgcaat gacccaggga ttgcggaaga aactgttgcc aacgctgaaa
     3781 tccactttgc gttcgaatga catgttgtgc gaaggcagcg aaaaggcgtt cacatccgcc
     3841 ttgcgtaccg tggtgtcacc gccggacttc accggatagt gattgtcagc aaacgcgcac
     3901 gggaatatcg ccggcaaaac cagcaggatc aaaacgaaca aaccccgcgg gaggcggggt
     3961 tggagtgaag gtgatgtcat caaaattcgt gttcggccgt atccgggttc agttcgtcga
     4021 tgccgatggt cttggccgct gtttcaatct ggcgggtgag tgccaccagc tgggcaattg
     4081 catccttgac caaggcctgt ccggctggat tgttttccgc aatcatctga tcgaacttca
     4141 tgggtgacgt ggtactttcc gcactgtcca ccagcttctg catggcggac atggttttgc
     4201 tgaactgcgc tttcacggcc ttgtcggtgt cgggggattt ggcggccacc agatcggaca
     4261 gcgaggcgcc cttcaccaca gaaccatcga tccgctggta gcggcccagg taaatgtttt
     4321 cgatgcccag cgcgtcatag aaatgggaat ggtgggtgtt gtcgctgaag cagtcgtgct
     4381 cgtcttccgg cgagttggct tccagcgcca ctttcagccg ctcgcccgcc agttcgccca
     4441 gcgccaggct gcccatgcca tacagcattt tcaccaggcc ggcatccggt ttgcctttca
     4501 gcaattcggc gcggtagttg tccttgccgg gagcccactg ctgttccatg ttggccagat
     4561 cgctcaccag caattgcgtg gccgccttca gataggccgc gcgacgatcg cagtgaccgt
     4621 tggtgcagcc ggcgccctgc acataatcgg tgtacgcacg gttgccggcg ccagggccgc
     4681 tgccgttcag atcctggccc cacagcagga attcgatggc gtggtagcca gtggcgacgt
     4741 tggcttccga cccgcccagt tcgttcaggc tggcgagcag ctccggcgtg atattcgcca
     4801 gatccagcgt gtcgctgccg agtttcaggc tggtgttggc aatgatgttg gcgttggcgc
     4861 cgacattgcc cagctcatgg tgatatttgc ccgtggcaac atagtcgatc agaccttcgt
     4921 ccagcggcca ggcattgagc tgcccttccc attcgtccac gttggtgttg ccgaaccgga
     4981 acacttcact ttgctggtag ggaacgcgcg ccgctttcca tgcggtgcgg gctttttgca
     5041 ggttggcgtc actgggggcc gacagcaggg cttccacact gccctgcagc gttttggcgc
     5101 tttgcaacgc gtcggcaaac acggcatggg ccacgttggc gtactgcgcc accacatcct
     5161 gttccggcgt ggcggccacg acgggaacag ccgtcagcag gcccaggccc accagcgcgt
     5221 gaagaatcgg tttcatgcag atgtctccgg tgaaaatgcc aaacagtcag atcagaagga
     5281 tgccgccagt tgcaccacga ggttgtcgct ctcctcgtcg ctgccgccat cggccaggcc
     5341 atagtccgtg tcgcgccaga tttcgccgcc cagcgtgacg ttccggtata tttcggtgga
     5401 acagccgagc gacatgcgtt cctctggcag ccccagcacc agcgcgtcct cggtctgctg
     5461 gtaacccgca gccagggtgt attcatgtcc cgccagggta gcggtaaagt ccagttccag
     5521 ttgcgccgct gcaggctcga tttcgatgcc gttggtagcg tccaccagcg gatcggccag
     5581 accgaggtat tcggcaatca gtaccactgc ccccagcgct atctggccgt ggacattgta
     5641 tgcaggcgcg gcatcgtccc attcaaacgc ggggtcgatc accggatcag cctctgccga
     5701 cagggtttcg ttcaggtcgg caaccccatc gctttcggta atgctggaga gataatcgac
     5761 gcccacctga aacgtttcct gcacgaagcc aacagtgaca ccgtagttct cggcatggcg
     5821 ttcacgttcc tggtcgccat caaatacgta agcgccggcc tcgatgccac tctgtgcgaa
     5881 cgcaaacagg gccgccgtct tgttggtttc cgccagttcc agcacccggg tgtcgttgat
     5941 cagtgcagtt tcaaacgtgc cgaagggcac atacatcttg cctacggtga agttcaccgg
     6001 gcccaccagt tcggcaatgg tcagggtggc ttcgtcaacg ccgaaatcgg tttgatcctg
     6061 ctcatagagg aacaccaggg cggcagacac cttgtcgttg atcgccgcat ccacgcccac
     6121 ttccactgtg gccaccgtca ggtcgctgta ggactggccg gcatagtcct cacccccgac
     6181 ggcttccact tccaccagac cccgcacctg caccgagtcc ggcaacgcgg caggcttcgc
     6241 cgactgctgt tctagggcgg tcaggcgctc ctcgacggtg ggctgggcct gggattgcgg
     6301 ctcagcgccg tatgcggtgg cggacagggc cagcaacagc gcagacaggg cctgcgcaac
     6361 cggcgggtgc aacttcgaca tacgaggaaa ctccatattc ccttgaattg tttggggtct
     6421 agaggacagg tgccgactat atatttagtg ataacaatta tcaactgcca gtttttcgca
     6481 ttccaatatt ggctttcagg aactcgccca tgcggcgcag ggcgcgccgg ccatccttca
     6541 gcaggaaggc ggtgtaatgc cagacatgct gcatgtcatc ccagacttcc agttgtaccg
     6601 gcaccccggc ctgggctgca cgctgcgcca cccggcgggc atcgtccagc aggatttcac
     6661 ggctgcccac ctggatcagc agcggcggca gcccggcaaa atccccctgc accggcgaca
     6721 ccagcggatc gttccagcgc tcttgtccga catagcgttc gccaaattgt tgcagcaaca
     6781 gcggtgtcag gatgaaatcc ttcgcggcat tgcgctgaat ggtttcaccc cccagcgaca
     6841 catcgcacca gggcgaaatc gggcaaccag cggcgggtaa cggcaggccc tcctgcttca
     6901 gtttcagcag cgtggtgagc accagattgc caccggcgga atcccccgcg aaaacgatgt
     6961 tcgccggctt gatgccctga tcgagaaaat agcggtagag acggaccgtg tcgtccagcg
     7021 cggcgggaaa cggatgctcc ggtgatttgc ggtaatcggg caccacggca cggataccgg
     7081 ccgcctgcgc caggcgcgac accagattgc gatggctgcg gggagatccc agacagaagc
     7141 cgccaccgtg aaaatacagc agcacctgat tgctgtgggc gtcgccgggt gtgtgaattt
     7201 cgcagtaaag tccggcgagg gtttcgttcg tcaccgtcac gccccgcacc ggcggcattt
     7261 tttcgcccca gcgatccatg cttttgcgcg accgcagcag ttcgccgtca gccacggcca
     7321 gtttgggacg gaacggggat ttcagcatca ggtccagcag gaaaacggac aaccggccgg
     7381 aagagctgcg cacggacgtt tcaattggag taggcattgt cgtaggcatt ggagactgga
     7441 ttccctcaga aaaagcggca acaggtaaaa cggcgcacaa ggctccggca ccttaacaaa
     7501 accacaatcg ggggcgcaat gctcagatcc gcatgagcgt ccggatatgc tggtaggcac
     7561 tgcgggacag tgcggtgaga ttgtagcccc cctccaggct cgacacgatg cggccattgc
     7621 agcagcggtc cgccaccagc atcagttcct cggtaatcca gcggtaatca cgttcgtcca
     7681 gatgcacgtc cgccagcggg tcagcctggt gggcatcgaa gccggtgctc atcatgatca
     7741 gctgcggatg aaaggtttcc agcttgttga gccactcgtt gcgcaccttg tcgcggtatt
     7801 catcgctgct ggtgccggcg tgcaacggtg tgtgaatgat gttggaacgg ctgttgtcgg
     7861 gcacctggta gggataacag ggatactgga agctggagca gaacagtacg cgttcgtcgt
     7921 attgcaggat gtcctcggtg ccgttgccgt ggtggacgtc gatatcgatg atggcaacgc
     7981 gatccagatt gtaggtctgc agcgcgtaag ccgcccccac cgccacattg ctgaatacgc
     8041 agaaacccat ggcacgccgc atttccgcat gatggccggg cggacgcacg gcgcagaacg
     8101 cattgccggc ctcgcccttc atcaccagat ccaccgcctt gcacacgcca cccgctgccc
     8161 tcagtgcggc gtccagcgtg tgggggttgg cgactacatc aggccccaga tcgcgcagat
     8221 cccgcagcgg aaactgctcg aagatcgcgt cgacataatc gcggctgtgc acgcgataaa
     8281 gatgtttctt ctcgattttc ggcgcatcca cctgcagcag cagatcccat atctgacgtt
     8341 ccatcaggta atcgcggatc gcgcgcaggc gggccggcgc ttccggatga ttaccaccca
     8401 tgtcgtgcaa ttcgcaatcc cggtgagtga tatacgcagt tgtcatgaaa gcgtcgcgac
     8461 ctcaggtttc agagtttttt gatgacatag accacatctt catcctggtc gtgatcacgc
     8521 agttcaaagc ccatggcccg cgcgagtttg cgcatgccgg tgttttcctt cagcacgatg
     8581 ccgtccatgt atttgaggcc cttctggcgc gccgcatcga tcagcaattc catcaactta
     8641 tacccgatac cggtgcgctg ccattgatcc gcgacaacga ctgcgaattc acagctggca
     8701 ccatcgggat tctgcacgta acggctgatg ccgatttcga tccacttgcc c
//
LOCUS       DASUEG010000829         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1623345, whole genome shotgun sequence.
ACCESSION   DASUEG010000829 DASUEG010000000
VERSION     DASUEG010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623345"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..491)
                     /locus_tag="VFX11_15640"
     CDS             complement(126..491)
                     /locus_tag="VFX11_15640"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4940113.1"
                     /translation="MEAQAPSQQNKRTVARRHLIYYLRVFNHHTGDLIGNLVDISTKG
                     IMVVSDKAIAPNTRYRLRMVLPDTVDGSKEVDFDAESRWCRNDANRDFFDTGFELLDP
                     SMEFLDAVDRLVEDCLFKE"
     gene            complement(564..1565)
                     /locus_tag="VFX11_15645"
     CDS             complement(564..1565)
                     /locus_tag="VFX11_15645"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEX4940114.1"
                     /translation="MSYILEALRKSEQERQRGKLPDLNSFKEEAAPAEKRLNPWPWVA
                     AGAVAINLVVALVVWAPWRGEDTLTVSTQPAPVDTPAGTVAPQPTTQTPPVQPAVAPQ
                     QAVAPVTAQQPAQAQMPQATGQQPVQPQYAQQPPAQPYGPSPTQYGSQPPPQGYPQPQ
                     QGYAQQQGYPQQMQPPPQPQQQFQSQYQAAYQQTYRAQTPTVTIMAPQVQDQPTVVSP
                     GGNAVAPSGEGEQYIEPYSPDFPPADTGRSPNVAYMPQLEELPPEERSAIPDMTFSSH
                     MYSSMPRFRSIIINGKRLKEGEYFNDTLQVREITDKGVIMSNGNTLFAVDVLGRWAQ"
     gene            complement(1565..>3181)
                     /locus_tag="VFX11_15650"
     CDS             complement(1565..>3181)
                     /locus_tag="VFX11_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4940115.1"
                     /translation="GEVGTGKTTVCRCFLEQIPADTDVAFVLNPKLEVEELLGTICDE
                     LSIPYIDDSVSIKDYVDCINIFLLRQHANGRHTVLIIDEAQNLSADVLEQLRLLTNLE
                     THEKKLLQIVLLGQPELQEMFRQPELRQLSQRVTARFHLNAMHAEEVGPYVYHRLAVA
                     GASEPRSMFPANVIARLHDISKGIPRVINLVCDRALLGAYARETRVVDLDILNTAAKE
                     VLGAEFFGSKGPRSWHWHPAAVGGMAGALVTGLIATVVFLFLNTGSDMPVADSAQRGV
                     PEPAAVVEDTPPPSVAVSATNTPQPDTNVAGQAQAAAVDSTVSMPAEPAVEPSLTLGE
                     LMQQAATPDEATAYVDLFKTWKLAFSPRINGDACKHAESVGLACLSKTGNLGSVRHLG
                     VPAMLKLYDQQGGEKYLVISQLEGNVARVYFNGEEKQADLSDLDTFWRGNYSLLWKKP
                     PSYHGPLEPGSIAPLSKWLAYQLDVWEKKPNPAMGRSSYDPVLVERVKAFQRSVGETD
                     DGVVGVSTLIQLSRRVDESVPALIAAEGGS"
BASE COUNT          611 a          934 c          955 g          681 t
ORIGIN      
        1 tcagcgcgcg ggcaaaatgc agattgtgct tgagtgcttc gagcgcgctc atttttttca
       61 actttatcaa tgacaattcg cccgactata aacaaaagcc gcggacgcgt catggcaaat
      121 ttgtatcatt ccttgaacag gcaatcttcc accagccggt ccaccgcatc gagaaattcc
      181 atcgacggat ccagcagttc gaaaccggta tcgaagaagt cccggttggc gtcgttgcga
      241 caccagcggc tttcggcgtc gaagtccact tccttgctgc catcgacggt atccggcagc
      301 accatgcgca gccggtaacg ggtgttgggt gcaatggcct tgtcggatac caccatgatg
      361 cccttggtac tgatatccac cagattgcca atcaggtcgc cggtatgatg gttgaacacg
      421 cgcaggtaat agatcagatg acggcgggca accgtgcgct tgttctgctg gctgggggcc
      481 tgtgcttcca tggttataac cttcaacgga gggtttccat taaggttaga cgagtaaacg
      541 gttttcttcc ggaacggcgt cgcttattgc gcccagcgac cgagtacgtc cacggcaaac
      601 agggtattgc cgttgctcat gatgaccccc ttgtcggtga tctcgcgtac ctgcagggta
      661 tcgttgaaat actcgccttc tttcaatcgc ttgccgttga tgatgatgga acggaaacgc
      721 ggcatgctgg agtacatgtg ggacgagaaa gtcatgtccg gaatggcgga gcgttcctcc
      781 ggtggcagct cttccagctg gggcatgtag gctacattgg gggaacgacc ggtatcagcg
      841 ggcggaaaat ccggcgaata gggttcgatg tactgttcac cctcaccgct gggcgcaacc
      901 gcgtttccgc cgggggacac cacggtgggt tgatcctgta cctggggcgc catgatggtg
      961 accgtggggg tctgggcgcg gtacgtctgc tgataggcag cctggtactg tgactggaat
     1021 tgctgttgcg gctgtggagg cggctgcatt tgctggggat aaccctgctg ttgcgcatag
     1081 ccctgttgtg gctgggggta gccctgcggc gggggttgtg atccgtattg tgtcggcgat
     1141 ggcccgtagg gctgcgcagg cggctgctgg gcatattggg gctgcaccgg ctgctggccg
     1201 gtggcctgcg gcatctgcgc ctgggcaggt tgctgtgctg tcacaggtgc gacagcctgc
     1261 tgcggagcca ctgcgggctg gaccggcggg gtttgtgttg taggttgggg agccacagtg
     1321 cctgccgggg tgtccaccgg cgcaggctgc gtcgaaaccg tcaaggtgtc ctcgccccgc
     1381 cagggtgccc agacaaccaa ggcaaccacc aaattgatag ctacagcacc cgcggccacc
     1441 cacggccacg ggttgagacg cttttcagcc ggagctgcct cttccttaaa gctgttgagg
     1501 tcaggcagct tgccccgctg ccgttcctgt tctgacttgc gcagggcctc cagaatgtag
     1561 gacatcagga acccccttcc gccgcaatca gggccggcac actttcgtca accctgcgcg
     1621 acaactggat cagcgtgctg acgcccacaa ccccatcgtc ggtctcgccc accgagcgct
     1681 gaaaggcctt cacccgttcc accagtacgg gatcgtaact ggagcggccc attgccggat
     1741 tgggtttctt ttcccacaca tccagctggt aagcgagcca tttggacagg ggcgcgatgg
     1801 agccgggttc cagcgggccg tggtaggacg gcggcttttt ccacagcagg ctgtaattgc
     1861 cgcgccagaa agtatccaga tctgacagat ccgcctgctt ctcttcacca ttgaagtaaa
     1921 cacgcgcgac attgccctcg agctggctga tcaccagata tttttcgccg ccctgctgat
     1981 catacagttt cagcatggcc ggtactccca gatggcgtac cgatcccagg ttgccggtct
     2041 tgctcaggca agccagcccg acggattctg catgcttgca ggcatcacca ttgatgcggg
     2101 ggctgaaggc caatttccat gttttgaaca ggtcgacgta ggcagtggcc tcgtccggcg
     2161 tggcggcctg ctgcatcagt tcccccaacg tcaatgaagg ttcgacggca ggctctgccg
     2221 gcattgacac agtgctgtcg actgcagcag cctgcgcctg tccggcgaca ttggtatcgg
     2281 gttgtggggt attcgttgct gacacagcca cactcggcgg tggcgtgtcc tctacaacag
     2341 cggcaggctc gggcacacca cgctgcgcac tgtcggccac gggcatatca ctccccgtgt
     2401 tgaggaacag aaaaaccacc gtagcaatca ggcccgtcac cagggcgcct gccattccac
     2461 cgacagcggc cggatgccag tgccaactac gcgggccttt tgaaccgaag aattccgctc
     2521 ccagcacttc cttggccgcc gtattcagga tatccagatc gaccacccgg gtttcccgcg
     2581 cataggcgcc cagcaaggcg cggtcgcaca ccaggttgat cacccggggg atacccttcg
     2641 aaatgtcgtg cagacgcgca atgacattgg ccgggaacat gctgcggggc tctgacgcgc
     2701 cggcaaccgc gagacggtgg tacacgtagg gcccaacttc ctccgcatgc attgcattga
     2761 gatggaagcg cgcagtaacc cgttgtgaga gctgacgcaa ttccggctgg cgaaacatct
     2821 cctgtaattc cggctgtccc agcagcacga tctgcagcag cttcttctcg tgggtttcca
     2881 gattggtgag cagccgcaac tgttccagca catccgcact gaggttctgc gcctcgtcga
     2941 tgatcagcac ggtgtgacgg ccattggcat gctggcgcag caggaaaata ttgatgcagt
     3001 cgacgtagtc cttgatggac acgctgtcat cgatataggg aatcgacagt tcgtcacaga
     3061 tggtccccag cagttcctca acctcaagct ttggattgag tacgaaggca acatccgtgt
     3121 ctgccggaat ctgctccaga aaacagcggc aaactgtcgt cttgcccgta ccgacttcgc
     3181 c
//
LOCUS       DASUEG010000830         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_938633, whole genome shotgun sequence.
ACCESSION   DASUEG010000830 DASUEG010000000
VERSION     DASUEG010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938633"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1360
                     /locus_tag="VFX11_15655"
     CDS             26..1360
                     /locus_tag="VFX11_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006868984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4940116.1"
                     /translation="MKCLVVGGGLAGLASAVWLAEAGHQVTVLERRGALGGRTIAIPQ
                     PEVDDVPDNGQHVFASGYENLFRYLDSVGTRQHVKFPGQMSIRMPGGEVRRSSFGGLD
                     GIRAMLGDLPGVSGLDKLRTARAQMKLIMQSLRQPADLDSITADEWFHRIGMPQSARR
                     AQWDGVVIGLTGDKPDISSAKVPADLIATGIRQAIKMRTAVSIGYPTVDLDTLFVTSA
                     EKVFAQRGVQVRTRAPVRSVDVENGRVKGVTLLDGEQIAADSVICAVPVWKIRGLLDQ
                     VPGHEQIYRAVEHLTPVPIVSVNLYLDRCIGMEDWGEILYDGEGVLEQVWDRQKMHGR
                     SPEKNWFYSTTVSASYDLIKGSNQAIIDTQMAMLRRYYPLARDAQVVHSHIVRMPYST
                     FAQRPGTAGVRPPQKTSVRGLAIAGDWTRTDWTTTMEGACQSAARAVEAVLA"
     gene            1390..2058
                     /locus_tag="VFX11_15660"
     CDS             1390..2058
                     /locus_tag="VFX11_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HEX4940117.1"
                     /translation="MRTRFLACLLLVFFSFAQASSWELKKDEDGIRVYVRDIKGSEFR
                     AFRGTVEVSSSLGAIVAVLKDGDGTRQWLKDCEDSKFLSEFDPAGYLMYFRTDAPWPV
                     KDRDYALRYKLEQDPQTLAVTLSFKGDASLTPQRDECVRVSDIEGAWHMTPQPNGKIL
                     LEYEVSADPNGSLPAWLANQFVVDQPFYTLKKFRKQIQLPQYRDQRFDFIREPTGSAS
                     TDAR"
     gene            2231..>3727
                     /locus_tag="VFX11_15665"
     CDS             2231..>3727
                     /locus_tag="VFX11_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX4940118.1"
                     /translation="MKNQRWKGLVRLLGIVWLGVWLAGCHEDLYVKVESGWLKGVWAD
                     ATKGNTRIFLGIPYATPPLGDLRFAPPQPAQRWRGVKRADQFGPSCVQRQGALSAPGA
                     QSEDCLTLNVYAPAQIPAGGAPVMVFIHGGAFIAGGSSQYNGRRLADTGNAVVVTLNY
                     RLGALGFFSHPDLDGGAALSGNAGLLDQQLALRWVKRNIRAFGGNPDRVTLFGQSAGS
                     ASVCVQMVAPGSRDLVQQFILESGVCTGGLQFLSQQQAQGISSAMAASLCPDADDRVA
                     CLRTLEPLQLMEWGATNTLFGAGWAPTVIPGSAVLPAPARELIAAGQYNPGPVMIGTT
                     LNEWGLFQSIGIAPPVSTVAQLSALIDSQFGPAAPALKAQYLPPVDALANLALIRLYT
                     DSVFRCPSRSFARQIQRQGSPVWLYSFEEGYAFHAMELPYVFGNPSPVLAPVLVEPLR
                     ETVQAYWTAFAATGEPSATNQPFWPPYDEVSDQHMVLKAASAAGAQLAQ"
BASE COUNT          645 a         1103 c         1257 g          722 t
ORIGIN      
        1 caactttgca aagggggttg acgatatgaa gtgtctggta gtggggggtg gactggcggg
       61 gctggcgtcg gcggtgtggc tggcggaggc cggccatcag gtgacggtgc tggagcgacg
      121 tggcgcgctg ggtgggcgca ccatcgcgat tccgcagccg gaagtggacg atgtaccgga
      181 caacggccag catgttttcg ccagcggcta cgagaacctg ttccgctacc tggacagcgt
      241 cggcacccgc caacacgtga agttccccgg gcagatgtcg attcgcatgc cggggggcga
      301 agtgcggcgt tcatcctttg gcgggctcga tggtatccgc gcgatgctcg gcgatctgcc
      361 gggcgtcagc gggctggaca agttgcgtac cgcccgtgcg cagatgaagc tgatcatgca
      421 atccctgcga cagccggcgg acctcgacag catcacggca gatgagtggt tccaccgcat
      481 cggcatgccg caatctgcac gcagggcgca gtgggacggc gtcgtcatcg gccttaccgg
      541 cgacaagcct gacatttcct ccgccaaggt tcctgccgat ctgattgcca ccggcatacg
      601 tcaggccatc aagatgcgca cggcggtttc catcggctat ccgacggtgg atctcgacac
      661 gctgttcgtt accagtgcgg agaaagtatt cgcgcagcgc ggtgttcagg tgcgcacccg
      721 ggcgcccgtc cgcagtgtgg atgtggagaa tggcagggtg aaaggcgtga cgctgctgga
      781 cggcgaacag attgcggcgg acagtgtgat ctgcgccgtg ccggtctgga aaattcgcgg
      841 cctgcttgat caggtgccgg gtcatgaaca gatctatcgt gcggtggagc atcttacgcc
      901 ggtgccgatt gtgagcgtga atctgtacct ggaccggtgc atcggcatgg aggactgggg
      961 cgagattctg tatgacggtg aaggtgtgct ggagcaggtg tgggaccggc agaagatgca
     1021 cggccgctcg ccggaaaaga actggtttta ttccactact gtgtcggcgt cgtacgatct
     1081 gatcaagggt tccaaccagg caatcatcga cacgcagatg gcgatgctgc gccgctacta
     1141 tccgctggcg cgcgatgccc aggtggtgca cagccatatc gtgcgcatgc cgtattccac
     1201 ttttgcgcag cgccccggta ccgccggtgt gcggccgccg cagaaaacgt cggtgcgtgg
     1261 tcttgcgatt gcgggtgact ggacgcgtac cgactggacg acgacaatgg aaggtgcgtg
     1321 ccagagtgct gcgcgggctg tcgaagcggt gctggcgtag tagacccttt ccatcaggag
     1381 tgccaagcca tgcgcacacg gtttcttgcc tgtctgctgt tggtgttttt cagtttcgcg
     1441 caggcgtcct cgtgggaatt gaaaaaagat gaggacggca tcagggtcta tgtgcgcgac
     1501 atcaagggct ctgaattccg cgcctttcgc ggtacggtcg aggtcagcag ttcgctgggc
     1561 gccattgtgg cggtactcaa ggacggtgac ggtacccggc agtggttgaa ggattgcgaa
     1621 gattcgaaat tcctgtcgga attcgatccg gccggttacc tgatgtattt ccgcaccgac
     1681 gcaccctggc cggtcaagga tcgcgattat gcgctgcgct acaagctgga gcaggacccg
     1741 cagacgctgg cggtgaccct cagtttcaag ggcgatgcca gccttacgcc ccagcgcgat
     1801 gaatgcgtgc gggtatcgga tatcgaaggc gcctggcaca tgacgccgca acccaacggc
     1861 aagatcctcc tggaatatga agtcagcgcc gatcccaacg gcagtctgcc ggcgtggctg
     1921 gccaaccagt ttgtggtgga ccagccattc tatacgctga agaaattccg caagcagatc
     1981 cagctgcccc agtaccgcga ccagcgcttt gattttattc gcgagccaac gggctctgcg
     2041 tcaacggacg cacgctgacg tcgccatgcc ctcccgaccg gccaagcggt caaagcgccc
     2101 ggtggccacg gccaatccag gccgcattcc gtgagtttgt cgccaccccg cccgtaggat
     2161 gttgacggcc attgcaccag cgcctgtgcg cggtgaatgc cgtgacatta caacggacga
     2221 gggaacaata atgaaaaacc aacgttggaa ggggctggtc cgcctgctgg gcatcgtgtg
     2281 gctgggcgtc tggcttgccg gctgtcatga ggatctttat gtaaaagtgg aatccggctg
     2341 gctcaagggt gtgtgggccg acgcgaccaa aggcaatacg cgaattttcc tgggcattcc
     2401 ctatgcgacg ccgccgctgg gcgatctgcg tttcgcgccg ccgcagccgg cgcagcgctg
     2461 gcggggagtg aaacgggcag accagtttgg cccttcctgc gtgcagcggc agggcgcgtt
     2521 gtcggctccc ggcgcgcaga gcgaggattg cctgacgctc aacgtgtacg cgccggcgca
     2581 gattcccgcc ggtggtgcgc cggtgatggt gttcattcac ggcggtgcct ttattgcggg
     2641 cggcagcagc cagtacaacg gccggcggct ggcggatacc ggcaacgccg tggtggtgac
     2701 gctgaattat cgtctgggtg cgctgggttt cttcagtcat ccggatctgg atggcggcgc
     2761 ggcgttgtcg ggcaacgccg gcctgctgga ccagcaactg gcgctgcgct gggtgaagcg
     2821 caatatccgt gcgtttggtg gcaatcccga tcgggtgacc ctgtttggac agtccgccgg
     2881 gtccgccagc gtgtgtgtgc agatggtggc gcccggcagt cgcgacctgg tgcagcaatt
     2941 cattctggaa agtggtgtgt gcactggcgg gttgcagttc ctcagccagc agcaggcgca
     3001 gggcatcagc agcgccatgg cagccagcct gtgcccggat gcggacgacc gcgttgcctg
     3061 tctgcgcacg cttgagccac tccagttgat ggagtggggt gccaccaaca ccctgttcgg
     3121 tgccggctgg gcgcccaccg tgatcccggg cagcgcggtg ttgccggcgc cggcccggga
     3181 actgattgcc gctggccagt acaaccccgg gccggtcatg atcggcacca ccctgaacga
     3241 gtggggcctg ttccagagca tcggcattgc gccaccggtc agcactgtgg cgcagctctc
     3301 tgcactgatc gacagccagt tcggccctgc cgcgcctgcg ctcaaggccc agtatctgcc
     3361 gccggtggat gctctcgcca atctggccct gatccgcctg tacaccgact ctgtgttccg
     3421 ttgcccctcg cgctcgtttg cccgccagat ccagcgccag ggcagcccgg tgtggctgta
     3481 cagctttgaa gaaggctacg ccttccacgc catggaattg ccctatgtgt tcggcaatcc
     3541 cagcccggtg ctggcgccgg tcctggtgga accgctgcgg gaaacggtac aggcttactg
     3601 gaccgcattt gccgccacgg gcgaaccgtc ggccaccaac cagccgttct ggccgcccta
     3661 cgacgaggtg tcggaccagc atatggtgtt gaaggctgcg tcggccgccg gagcccagct
     3721 ggctcaa
//
LOCUS       DASUEG010000831         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_452781, whole genome shotgun sequence.
ACCESSION   DASUEG010000831 DASUEG010000000
VERSION     DASUEG010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452781"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..181)
                     /locus_tag="VFX11_15670"
     tRNA            complement(91..181)
                     /locus_tag="VFX11_15670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Ser,seq:gga)
     gene            392..2275
                     /locus_tag="VFX11_15675"
     CDS             392..2275
                     /locus_tag="VFX11_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX4940119.1"
                     /translation="MQQPSSHNAKTPGIALALGALGVVFGDIGTSPLYALKECFHAGH
                     GIPISFENVVGILSLIFWSLVTVVSLKYVAFIMRADNNGEGGILSLLALALRSENKAG
                     WRRSLLIGVGLFGAALFFGDGMITPAISVLSAVEGIKVAAPVLDPYVIPITIIVLVSL
                     FLIQRHGTASVGAFFGPVMLVWFLTLGWLGIINISRHPDVLNLINPIWAFRFVLEQQV
                     IAFIVMGAVVLTVTGGEALYADMGHFGRKPIYRAWFFVVFPALVLNYCGQGALLLADP
                     SAVENPFYLLAPDWALYPMIGLATLATVIASQAVISGVFSVTRQAVQLGYLPRMDIQH
                     TSSKEIGQIYIPSINWALLAAIIVLVLTFRTSSNLASAYGIAVTMTMICDTFLAMFVA
                     YHLWKWKPASILAIGIPFLAIDFTFFGATSLKIVQGGWVPLTIGVVIALLMSTWKRGR
                     TLLFDRLSQETMPLNLFVNSIGGGSVQTVPGTAVFMTGSHQYVPHAMLHNMKHNKILH
                     ERNVLLTLVTRDIPFVSEDDRFLVTEVAHNFYLITGYYGFKEQPDVPALLAQCHRMGL
                     EFDMMDTSFFLSRERIINTVPGLTGWRRKLFVAMSRNAGAATDFFQIPTNRVVELGTQ
                     VEL"
     gene            2413..3276
                     /locus_tag="VFX11_15680"
     CDS             2413..3276
                     /locus_tag="VFX11_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX4940120.1"
                     /translation="MRTDNQRRSSNIEDRRGNRLPGGKGGLGLGAIVIALVAAYFGID
                     PQVAMQVANTVAPPSQEMPANTPVTESATEAAARDLVSVVLADTEDTWHALFRAGGGT
                     YREPTLVLFRDATSTACGTGQSAMGPFYCSADEKVYIDLSFYDQLHSRFRAPGDFAQA
                     YVIAHEVGHHVQHLLGISGKVQRARQQVSEKEGNALSVRLELQADCLAGVWAHHAQRS
                     RQLLESGDLEEALTAATAIGDDTLQKNAQGYAVPDSFTHGTSQQRVQWFRTGMEAGSL
                     DACDTFAANVR"
     gene            3509..>4328
                     /locus_tag="VFX11_15685"
     CDS             3509..>4328
                     /locus_tag="VFX11_15685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4940121.1"
                     /translation="MALAADSPEEGSPLLEYTLTELLSLHVETASRSIEALDRAPATV
                     YVITADDIRTYGYRNLADALENVPGVMGINKSFFLDGGQRGLFGNFSQTLLLINGREF
                     QGLATQEAFISDQFATDNIRQIEVMNSPGSVLYGANAYSGVINILTRDADSDFTGQQF
                     RITQGSENTQALSLVSATRTSLGRFSVYLRQYRSDNWDFGEAVADRSHFSAGYPAVFQ
                     DASAQAGREFMSRSRARSWAARFERNGFYLGTDGYQLENGKGLEAVAMDYDSQRD"
BASE COUNT          823 a         1400 c         1220 g          885 t
ORIGIN      
        1 gtactgcgtg gtatccacaa aggggcatta cccctttaac gcattgagct ttattttgaa
       61 gcccttgaga gccaagggct tgaaggaatc tggcggtgag ggagggattc gaaccctcgg
      121 tacgctatta acgtacacac gctttccagg cgtgcgcctt caaccactcg gccacctcac
      181 cagtcccgca acagctgccc gtcgcgaggc gcgcaatcat atacgagtgg ggacaaaagg
      241 caaagccgcc tgaacggttt ttatcacctt gcccaggggt gttgcccgca tgggggcagg
      301 tgtaagatgc gccctttgcc aaggccagac accagcgtac cggcctgctg cccgtgtatc
      361 cggcagcaag gacggcaacg aggaagccct catgcagcaa cccagctcac acaatgccaa
      421 gacccccggc attgccctcg ccctcggcgc cctcggcgtc gtcttcgggg acatcggtac
      481 cagcccgctc tacgccctga aagaatgctt ccacgccggt catggcatcc cgatcagttt
      541 cgaaaatgtg gtcggcatcc tgtccctgat cttctggtca ctcgtgacgg tggtctcgct
      601 caaatatgtc gccttcatca tgcgcgccga caacaatggc gaaggcggca tcctgtccct
      661 gctggcactg gctctgcgta gcgagaacaa ggccggctgg cgtcgcagcc tgctgattgg
      721 ggtcggcctg tttggagcgg ccctgttctt cggcgacggc atgatcacgc cggccatttc
      781 cgtgctgtcg gccgtcgagg gcatcaaggt ggccgccccc gtgctcgacc cctacgtcat
      841 ccccattacg atcatcgtgc tggtcagcct gtttctcatc cagcgccatg gcaccgcctc
      901 ggtcggcgcc ttctttggcc ctgtcatgct ggtctggttc ctgaccctgg gctggctcgg
      961 catcatcaac atcagccgcc acccggacgt gctcaacctg atcaacccga tatgggcttt
     1021 ccgcttcgtg ctggaacaac aggtgatagc gtttatcgtc atgggtgccg tcgtgctgac
     1081 cgtgacgggg ggcgaagccc tgtatgcgga catgggacac ttcggtcgca agccgatcta
     1141 ccgcgcctgg tttttcgtcg tctttccggc actggtcctg aactactgcg gccagggcgc
     1201 cttgttgctg gccgacccgt ctgccgttga gaaccccttc taccttctgg cccccgactg
     1261 ggcgctctac cccatgatcg gtctggccac gctggctacc gtgattgcct cgcaggccgt
     1321 catctcgggc gtgttctcgg tgacccgcca ggccgtccag ttgggctacc tgccgcgcat
     1381 ggacatccag cacacctcca gcaaggagat cggccagatc tacattccgt cgatcaactg
     1441 ggcgctgctg gccgccatca tcgtgctggt gctgaccttc cgtacctcca gcaacctggc
     1501 ctcggcctac ggtatcgctg tcaccatgac gatgatttgc gacaccttcc tggccatgtt
     1561 cgtggcttat catctgtgga agtggaagcc agcatccatt ctggccatcg gcattccctt
     1621 cctggccatc gacttcactt tctttggcgc cacctcgctc aagatcgtcc agggtggctg
     1681 ggtgcccctg accatcggtg tggtcatcgc cttgctgatg tccacctgga agcgcggccg
     1741 caccctgctg ttcgatcgcc tgtcgcagga gaccatgcca ctcaacctgt tcgtgaactc
     1801 gatcggtggc ggctcggtgc aaaccgtccc ggggaccgcc gtattcatga cgggcagcca
     1861 ccagtacgtg ccgcacgcca tgctgcacaa catgaagcac aacaagatcc tgcacgaacg
     1921 caacgtactg ctgacactgg tcacccgcga cattccgttt gtgtcggaag acgaccgctt
     1981 tctggtcacg gaagtggccc acaatttcta cctgattacc ggctactacg gtttcaagga
     2041 acagccggac gtgcctgctc tgctggccca gtgccaccgc atggggctgg agttcgacat
     2101 gatggatacc agcttcttcc tgtcgcgtga acgcatcatc aacaccgtgc ccggcctcac
     2161 cggctggcgg cgcaagctgt tcgtggccat gtcgcgcaac gccggcgctg ctaccgactt
     2221 cttccagatc cccaccaacc gcgtggtgga actgggcacc caggtcgagc tgtaagccat
     2281 ccagtctctc ccccaaagcc cggttcgccg ggcttttttg tgcctggcca acggcccgct
     2341 gctagactgg acgcaaccaa cagattgccc gggaccacct tgggatggtc acaaagggct
     2401 tgaggaaccg ggatgcgtac ggacaaccag cgtcgcagca gcaacattga agacaggcgc
     2461 ggcaaccgtc tgccgggcgg taagggtggc cttgggctgg gcgccatcgt tatcgcactg
     2521 gtcgccgcct acttcggtat cgacccacag gtggccatgc aggtcgccaa cacggtagcg
     2581 cctccctccc aggagatgcc ggcaaacacc ccggtcaccg aatctgccac tgaagctgcc
     2641 gcacgcgact tggtcagcgt ggtgctggcc gataccgaag acacttggca tgccctcttc
     2701 cgtgcgggtg gcggcaccta tcgggagccg acactggtcc tgttccggga tgccacctcc
     2761 accgcctgcg gcaccggcca atccgccatg gggccgttct actgttcagc cgatgaaaag
     2821 gtttacatcg acctgtcgtt ctacgaccag ctccacagcc gcttccgggc acccggggat
     2881 tttgcgcagg cctatgtgat tgcccatgag gtcggtcatc acgtccagca cctgctcggc
     2941 atttccggca aggtacagcg ggcacgccaa caggtcagcg aaaaggaggg gaatgcgctg
     3001 tcggtacgcc tggaactgca agccgactgc ctggctggcg tttgggccca tcacgcccag
     3061 cgatcccggc aactgctgga aagtggggat ctggaggagg ctctgaccgc tgccactgcg
     3121 attggtgacg acaccctgca gaaaaatgca cagggctatg ccgtgcccga ctccttcacc
     3181 catggcacct cgcaacagcg cgtgcagtgg ttcaggaccg gaatggaggc gggaagtctc
     3241 gatgcctgtg acacatttgc cgccaacgta cgctaacagg agagcaaccc cagagtcggc
     3301 catgcagtcc agcgctgagc aagatgtcac gagccgtcct tgtacctgcc gttaacaatg
     3361 tatccttgag caatgatgct tgtgtgttgg cgcaatccac tggctgtgcc tatccttcca
     3421 ccaaggtcac cactctgttc tgcgatcaac aataatgaca aagcgcccag ccaggctatt
     3481 gctggctatc gcactcatca gcccaagcat ggcactggca gcggactccc ccgaggaagg
     3541 ctcgcccctg cttgagtaca cgctcactga gctgctgagc ctgcatgtgg aaaccgccag
     3601 ccgcagcata gaggctctgg atcgtgcacc tgccaccgtg tatgtgatta ccgctgacga
     3661 tatccgtacc tacggctacc gcaatctggc cgacgcactg gaaaacgtgc ccggcgtcat
     3721 gggcatcaac aagagctttt ttcttgatgg cggccagcga ggcctcttcg gcaacttctc
     3781 gcagactctg ctgctcatca acggacgtga atttcagggc ttggccactc aggaagcctt
     3841 catcagcgac cagtttgcga ctgacaacat tcgccagata gaagtcatga acagcccagg
     3901 ctccgtgctc tatggggcaa atgcctacag cggcgtcatc aacatcctca cccgggatgc
     3961 cgattccgat ttcacgggcc agcaattccg tatcacccag ggcagcgaaa acacacaagc
     4021 actcagcctg gtcagtgcca cccggacctc cctcggccga ttttccgtct accttcgcca
     4081 ataccggagt gacaactggg attttggtga ggcggtcgcg gatcgctcac acttttccgc
     4141 cggctacccc gcagtatttc aggatgcttc ggcacaggca ggtcgtgagt tcatgagccg
     4201 cagcagagca cgtagctggg ctgcgcgttt tgagcgaaac ggtttttatc tggggactga
     4261 cggttaccag ctggaaaacg gcaaggggct ggaagccgtg gccatggact atgactcaca
     4321 acgtgata
//
LOCUS       DASUEG010000832         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1910597, whole genome shotgun sequence.
ACCESSION   DASUEG010000832 DASUEG010000000
VERSION     DASUEG010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910597"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..301
                     /locus_tag="VFX11_15690"
                     /pseudo
     CDS             59..301
                     /locus_tag="VFX11_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(376..2739)
                     /locus_tag="VFX11_15695"
     CDS             complement(376..2739)
                     /locus_tag="VFX11_15695"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEX4940122.1"
                     /translation="MTGNRLLLIDDEDAIAKTVKVIANGCGFDVETTSDAAIFLERVA
                     SWPPSHVVLDLQMPKKDGVELLREMSDLGYSGRIIIASGAGGRIVDAARRLGVERGLD
                     IVATLLKPFRAAEMRDLLTRLAIADPIWESETALRLAIAQNQIFLCYQPKIDLKTGRM
                     VGLEALVRWRHPVQGDLFPDQFIPLAERSGLIQDLTWKVIGIGLDQLAEWGATVDASL
                     AINLSGASLRDLSFVDQLNEQCVIRGVNPGRIVLELTETFAMADPVNAMEILTRLRLK
                     GFALSIDDFGTGYSSLSQLARLPFSEMKVDKSFVMQADSSPEARVIIKSIIDLAHNLG
                     LHVIAEGVESAAIQKIVTDLGCDLAQGYHISRPLTGDKIIPWLQGWQASQALEIDTVE
                     EKAASQPLHSPWIKHYDGSDDAKAALTEFLSERINPLWRLGRDSLIGWRITGSGIEVL
                     MAPYQNIVDHFEKLERLLRGARMMGDRTFQLAHQLVGYTPAPIELPFRISDDEPGAVP
                     TGMIEQVLCRYAITETQHRAVTLFDISAFSRYESRVQVSQLNSLECSINTAQGILREA
                     GKTIDLARTTAGDGFYIWNRLKGPQADLDTYLLTLLVVVDNAIARLGGREPFVPEIRT
                     CFSVGPHYSYHQVDGLDPRGHDYIVGDVTIGLARMISKCLPGQILIGSFNRPSDDDTD
                     PTNPFDFAIEADSVFDRLDNFRMHGRLVSGIRCYLTGEERAPDMFDVTRYRILDKHGF
                     EHDVFNQKFNIYLIDDPAIDSIKALYVGKQHAELDAFDAIPSQVSLV"
     gene            complement(2777..>3701)
                     /locus_tag="VFX11_15700"
     CDS             complement(2777..>3701)
                     /locus_tag="VFX11_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4940123.1"
                     /translation="LQPMAFDLNELASGTTNLLRRTLGEQIEVETVLASDLWLALADP
                     EQMETALTNLAINSRDAMPNGGRLTIETANRRLDEEYAAANAEVTPGDYVMLALTDTG
                     EGMSPEIVNRVFEPFFTTKEPGKGTGLGLSMVYGFVKQSHGHIKIYSEPGYGTTIRLY
                     LPRAQEDLVQKDREAGDKTEKTAIGAMILVVDDNPDVRSIVVKQLADFGYSVMESQDG
                     PSALDIIRNDETIDLLFTDIVMPGGMTGIELAREARKIRPDIKVLLTSGFSEAMVQND
                     GQPGETINVLSKPYRSQELARKIQEVLSGKP"
BASE COUNT          811 a         1068 c         1000 g          822 t
ORIGIN      
        1 ttgtaacggt tttgttccag ttgcttgagg gtgtattttc acatcaagga gatcaggaat
       61 ggctatcaag cataatgaag aattcaagcg agaagcggtg catatagcgc tgagcagcgg
      121 tttgccactg catcaggttg cggctgatct gggtgttgga ttatcgacgc tgggcaaatg
      181 gttatcgctg cgtcggcccg atgaccgccg cgctggcctg gatgatgatc tggccagcga
      241 gaatgaacgg ctgcgccggg agaatcgtgt actccgggag gagagggaga tattaaaaaa
      301 ggcgccgccg ttttctttgc gagccaaaag ccgggaacga aatggcagac atgtcatgaa
      361 tgcttatggc tggtctcaga ctagtgatac ctggctcggg attgcgtcaa acgcatcaag
      421 ctcagcatgt tgcttgccta catacagcgc tttgatagaa tcgatggctg gatcgtcaat
      481 cagataaatg ttgaattttt gattgaatac atcgtgttcg aagccatgtt tgtcgaggat
      541 gcggtatctc gtcacgtcga acatgtcggg tgcgcgttct tcgcccgtca gatagcagcg
      601 gatgccgctc accagacggc catgcatccg gaaattgtca agcctgtcaa agacactgtc
      661 cgcctcgatc gcaaagtcga acgggttggt tggatcggtg tcatcatccg agggccggtt
      721 aaagctgcct atcagtatct gtccaggcaa acatttcgag atcatgcggg cgagccctat
      781 ggtgacgtca cccacaatgt agtcgtgccc gcgcgggtcg agcccatcga cctggtggta
      841 ggaataatgc ggtccgaccg agaagcaggt cctgatctcg ggcacgaacg gctcccggcc
      901 gccaagccgc gcgatagcat tgtcaaccac aaccagcagg gtgagcagat aagtgtcaag
      961 atcggcctgc ggccccttca gccggttcca gatataaaaa ccatccccgg ccgtggtgcg
     1021 ggcgagatca atcgtcttgc cggcttcccg cagaattcct tgagcggtgt tgatcgagca
     1081 ttccaggctg ttgagttgcg acacctgcac ccgggactcg tagcgcgaaa aggctgagat
     1141 atcgaacagc gtgaccgcgc gatgctgcgt ttcggtaatg gcgtagcggc acagcacctg
     1201 ctcgatcatt ccggttggga cggcgccagg ctcatcatcg ctgatacgaa acggcaactc
     1261 aataggtgca ggcgtatatc caaccagctg atgcgcaagc tggaaagtcc gatcccccat
     1321 catccgggcg ccgcgcaaca gccgctccag tttttcgaaa tggtcgacga tgttttgata
     1381 aggcgccata agcacctcta tgccgctacc ggtgatacgc cagccaatca gggaatcgcg
     1441 gcccagccgc cacaaaggat ttatgcgttc ggatagaaac tcggtcagcg ccgctttcgc
     1501 gtcgtcggag ccgtcataat gcttgatcca gggcgagtgc aggggctggg acgctgcctt
     1561 ttcctcaacc gtatcgattt ccagagcctg gctcgcctgc cagccctgaa gccagggaat
     1621 gatcttgtcc cccgtgagcg gccggctgat atgataacct tgcgccaggt cacacccaag
     1681 atcggtcacg atcttctgta tggccgcgct ttcgacaccc tcagcaatca catgcaagcc
     1741 gagattgtgc gccagatcaa tgatcgattt gataatcacc cttgcttctg gcgaactgtc
     1801 cgcctgcatg acgaagctct tgtcgacttt catttcggaa aacggcaggc gggcgagttg
     1861 cgacagcgat gaatagcccg tgccaaagtc atcaatcgaa agcgcaaatc ccttgagccg
     1921 gagccgcgtc aatatttcca tggcgtttac cggatcggcc atggcgaaag tctctgtcag
     1981 ttccagaaca atacgcccgg gattgacgcc gcgtataacg cattgctcgt tcaactggtc
     2041 cacgaaagac agatcacgca gactggcgcc ggaaagattg atggcgagcg acgcgtccac
     2101 agttgcgccc cattctgcaa gctgatccag gccgatcccg atcaccttcc aggtcagatc
     2161 ctggatcagc ccgctgcgct ccgccagggg aatgaactgg tccgggaaca gatctccctg
     2221 caccgggtgc cgccagcgca ccagcgcctc aaggcccacc atgcggcctg ttttcaggtc
     2281 gattttcggc tggtaacaca gaaaaatctg attttgggca atcgccagcc tgagggcggt
     2341 ttcgctctcc cagatcggat cggcaatggc caggcgggtg agcaaatcgc gcatctcggc
     2401 ggcgcgaaac ggtttaagca gcgtggccac gatgtcgagc ccgcgttcca cccccagccg
     2461 tctggcggcg tcaactatcc gcccccccgc gccgctggca atgataatac gccccgaata
     2521 gcccaaatca ctcatttcac gcagcaactc aacgccatct ttcttcggca tttgcaaatc
     2581 aagaacgaca tgggatggcg gccaactggc gacacgctcc agaaatattg cggcatccga
     2641 agttgtttcc acatcaaaac cgcagccatt tgcgatcact tttaccgttt tcgcgatggc
     2701 gtcttcatcg tcaatcagca atagccggtt accggtcatg acacttcctc ctctgcgcct
     2761 gatttctttt ttgcctttat ggcttaccgg ataaaacctc ctgaatcttc cgggccagtt
     2821 cctggctgcg gtatggcttg cttaaaacat ttatcgtctc gccaggttga ccgtcatttt
     2881 gaaccatcgc ctccgaaaag cccgaagtaa gcagaacctt tatatccggc cttattttcc
     2941 gcgcctcgcg ggccagttca atgcctgtca taccgcccgg catcacaatg tccgtgaaca
     3001 gcagatcaat cgtctcatcg ttgcggataa tgtcaagcgc tgatgggcca tcctgtgatt
     3061 ccatcacact gtagccaaaa tcggcaagct gtttgaccac aatgctgcgc acgtcagggt
     3121 tgtcatcaac gacaagaatc atggctccaa tggccgtctt ttcagtcttg tcgcccgcct
     3181 ccctgtcttt ctgaaccagg tcctcctgcg ccctcggcaa ataaaggcgg attgtcgtgc
     3241 cgtaaccggg ttcgctatag atcttgatgt gcccatggga ctgcttgaca aagccataga
     3301 ccatactgag tccgagacca gtccccttgc ccggctcctt ggtcgtgaaa aaaggttcaa
     3361 acacccggtt aactatctcg ggggacatgc cctcacccgt atccgtgaga gcgagcatca
     3421 cataatctcc cggcgtaacc tccgcgtttg cggcggcata ttcttcatca aggcgccggt
     3481 tggctgtctc gattgtcagc ctgccgccat tgggcatggc gtcgcgcgaa ttgatggcga
     3541 gattggtaag cgcggtttcc atctgttccg gatcggcaag agccagccac agatcgctgg
     3601 ccaggaccgt ctcaacttca atctgctcgc caagcgtccg gcgcagcaga tttgtcgtgc
     3661 cggacgccag ttcattaaga tcgaacgcca tcggctgcag g
//
LOCUS       DASUEG010000833         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_784180, whole genome shotgun sequence.
ACCESSION   DASUEG010000833 DASUEG010000000
VERSION     DASUEG010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784180"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFX11_15705"
     CDS             <1..293
                     /locus_tag="VFX11_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4940124.1"
                     /translation="HLHALITLFRRGAQQRNIPLSESSTAIQPLIVGASDKALRLSEQ
                     LLQQNILVGAIRPPTVPANTARLRITLTAAHTESDIEQLLDALHAALLSCKE"
     gene            297..1100
                     /gene="bioH"
                     /locus_tag="VFX11_15710"
     CDS             297..1100
                     /gene="bioH"
                     /locus_tag="VFX11_15710"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEX4940125.1"
                     /translation="MALTLYHDTYAHTGTRENPVDLVLLHGWGLHSLVWDAVMPALLE
                     QFRVTVIDLPGLGRSPVPGGDYNLDYLVEHVLAVAPHNAVWIGWSLGGMVAMRIAARF
                     PERVKALITVASTPQFVADERWPAALKPELLQGFRNLLDEDWEGTLIRFLALQCKDSE
                     TMRDDVRTLKEIVYFHGLPAQKALRCGLEILRDVRLLDELQKIQCPTLHVFGEKDNLV
                     PVSASSAVRQFQPDAKTAVIKGVSHVPFLSAPDLFIAACRDFFSEQLLA"
     gene            1109..2005
                     /gene="bioC"
                     /locus_tag="VFX11_15715"
     CDS             1109..2005
                     /gene="bioC"
                     /locus_tag="VFX11_15715"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEX4940126.1"
                     /translation="MQVTGDLEPQGSQPAGVVRPIAENRPTPENVVSVNKRAVAQSFS
                     RAAQQYDQVAQLQMQVGQQLLTAIDLPDSPRRKNMALDIGCGTGNLTRHLQPYCDTLV
                     ALDFAPGMLQFASAHHSDSIQHFICADADALPFAPASFDLVFSNFALQWCESLPALFR
                     NLFALLRPAGQLVFSIPGDGTLWELQHSWQQADPFHRHVNTFVTAQQVRDGLQHAGFQ
                     LDAVQAEPVIMQYASVRELTHELKTLGAHIVNGERNRQLTGKRAVAGMLQAYEQLRNG
                     DGRLPATWNIIRASAHKPAFPA"
     gene            2035..2748
                     /gene="bioD"
                     /locus_tag="VFX11_15720"
     CDS             2035..2748
                     /gene="bioD"
                     /locus_tag="VFX11_15720"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX4940127.1"
                     /translation="MKKARFFITGTDTDAGKTVATLALLHAFKQQGLQTVALKPIAAG
                     CEMVDGELRNADALQLQNAATVHLPYDQVNPVALKDAVAPHIAASREGRKLLASRIVG
                     LVRGALMVPADVYLVEGAGGWYVPLNERETQANLVKDLQIPVILVVGMRLGCLNHAIL
                     SARSIQLDGVRVAGWIANCVQPASAPYMHGPELDANVAILRDMLSAPCLGVIPYCQPL
                     DLDGMAASIQTDLLLKPYS"
BASE COUNT          566 a          892 c          889 g          602 t
ORIGIN      
        1 cgcatctgca tgcactgatt accttgttcc gccgaggcgc gcagcagcgg aatattccgc
       61 tgtctgaatc gtccactgca attcagccgc tgattgtcgg tgcctcagac aaagcactgc
      121 gtctgagcga acaactgctg caacagaata ttctggtggg tgcgatccgt ccgcccaccg
      181 tgccggccaa caccgcgcgc ctgcgtatca cgctgacagc cgctcacact gaatccgata
      241 ttgaacaatt gctcgatgcg ctgcatgcgg cgctgttgtc ctgcaaggaa tgaatcatgg
      301 ccctgaccct gtaccacgac acctatgcac acactggcac ccgggaaaat cccgtcgatc
      361 tcgtgctgct gcacggctgg ggcctgcata gtctggtgtg ggacgccgta atgcccgcgc
      421 tgctggaaca gtttcgggtg actgtgattg atctgccggg tctgggtcgc agcccggtgc
      481 cgggcggcga ctacaacctg gattatctgg tggagcatgt gctggcggtg gcgccacaca
      541 atgcggtctg gatcggctgg tcccttggtg gcatggtggc gatgcggatt gccgcgcgat
      601 ttcccgagcg ggtaaaagca ttgatcaccg tcgccagcac accgcaattc gtcgccgatg
      661 agcgctggcc tgctgcattg aagccggaat tgctgcaggg cttccgcaat ctgctggatg
      721 aagactggga aggcaccctg atccgtttcc tcgccctgca atgcaaggac agcgagacca
      781 tgcgcgatga cgtgcggacg ctgaaagaaa ttgtctactt ccacggattg cccgcacaaa
      841 aggcgctgcg ctgcgggctg gaaatcctgc gcgatgtgcg tttgctggat gaactgcaaa
      901 aaatccagtg ccccacgctg cacgtgttcg gtgagaaaga caatctggtg ccggtgagtg
      961 ccagcagcgc cgtgcgccag tttcagcccg acgccaaaac cgccgtgatc aagggggtga
     1021 gtcacgtgcc gttcctgtcg gcgccagacc tgttcattgc cgcctgtcgc gattttttca
     1081 gcgagcagct gctggcatga taagcgcgat gcaagtcacc ggtgatctgg aaccgcaggg
     1141 cagtcagcct gctggtgtgg tgcggccgat cgcggaaaac cgaccaacac cggaaaatgt
     1201 cgtcagcgtc aacaagcgtg ccgtggcaca aagtttcagc cgtgccgcgc agcagtacga
     1261 ccaggttgcg cagctgcaga tgcaggttgg gcaacagttg ctgacggcta tcgatttgcc
     1321 agattcaccg cgcaggaaaa acatggcgct cgacatcggt tgtggcaccg gcaatctcac
     1381 ccggcatctg caaccgtatt gcgatacgct ggtcgccctg gatttcgccc ccggcatgtt
     1441 gcagtttgcc agcgcgcacc acagcgacag catccagcac tttatctgcg ccgatgccga
     1501 cgcgctgccg ttcgcacccg ccagtttcga tctggtgttc agtaattttg ccttgcagtg
     1561 gtgtgaatct ctgccggcgc tgttccgcaa tctgtttgcc ctgttgcgtc ccgctggcca
     1621 gctggtattt tccattcccg gcgatggcac cctgtgggaa ttgcagcaca gctggcagca
     1681 ggccgatccg ttccaccgtc acgtcaatac attcgtaacg gcacagcagg ttcgcgacgg
     1741 gctgcagcac gccggcttcc agctggatgc cgtgcaggca gagccggtga tcatgcagta
     1801 cgccagcgtg cgggaattga cgcacgaact gaagacgctc ggcgcgcata tcgtgaatgg
     1861 cgagcgcaat cgccagctta ccggcaaacg cgcggtggcg ggcatgctgc aggcctatga
     1921 acaattgcgt aatggcgacg gcaggctgcc cgccacctgg aacatcatcc gtgccagcgc
     1981 ccacaaaccg gctttccccg cttgagcctc accactatcc aggactgtcc atccgtgaaa
     2041 aaagcccgct tcttcatcac cggaaccgat actgacgccg gcaagactgt tgccacgctt
     2101 gcgctgctgc acgcattcaa gcagcagggg ctgcaaaccg ttgccctgaa gcccatcgcc
     2161 gccggctgcg aaatggttga cggtgaactg cgcaatgccg atgcgctgca gctgcagaat
     2221 gccgccacgg ttcatctgcc ctatgaccag gtgaatcccg tggcactgaa ggatgcggtg
     2281 gcgccccata tcgccgccag cagggaaggg cgcaagctgc tggcatcccg tatcgtcggg
     2341 ctggtgcgcg gcgcgctgat ggtgccagcg gatgtctacc tggtggaagg cgccggaggc
     2401 tggtacgtgc cgctgaacga acgtgaaacc caggccaatc tggtgaagga cctgcagatc
     2461 ccggtgattc tggtggtggg tatgcggctg ggctgtctca atcacgccat cctgtcggcc
     2521 cggtccatcc agctggatgg cgtgcgggtg gctggctgga tcgccaactg cgtgcagccg
     2581 gccagcgcac cctatatgca cggccccgag ctggacgcca atgtcgccat cctgcgggac
     2641 atgttgtccg ccccctgtct gggggtgatc ccctattgcc agccgctgga tctcgacggc
     2701 atggcagctt cgatccagac ggatttgcta ttaaaaccct atagttagag gccatccatc
     2761 gcactgtctc cgtcgttcca gctggagcag ttgcgtcctt ccatggctat aaattgatca
     2821 ttattcgcgc agtttgactt cctgaactat actgcaggca tagccctgaa accgtcccgg
     2881 ttcggggtat gaaccgctca gaggttgcag tatggcgatt ggttcgatca tgggaaatgg
     2941 cgttgccgg
//
LOCUS       DASUEG010000834         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2166530, whole genome shotgun sequence.
ACCESSION   DASUEG010000834 DASUEG010000000
VERSION     DASUEG010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166530"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="hflX"
                     /locus_tag="VFX11_15725"
     CDS             <1..697
                     /gene="hflX"
                     /locus_tag="VFX11_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4940128.1"
                     /translation="IPLVTLVGYTNAGKSTLLNRLAQADVYVADQLFATLDPTVRRIE
                     VPDLGGIILADTVGFIRHLPHKLVEAFRATLEETRDADFLLHVVDACAVDREDNIFQV
                     QEVLAEIGADDVPGVMVYNKIDMMGDFPPRIDRDEEGTATAVWVSAVTGAGLDLLLQV
                     LNERLGKKMVEGVLRLLPEQSAVRAHCYELKAVQDESADDSGNSLLSIRMPVKAWQQM
                     LKRFGLQGDILID"
     gene            798..1949
                     /gene="hflK"
                     /locus_tag="VFX11_15730"
     CDS             798..1949
                     /gene="hflK"
                     /locus_tag="VFX11_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX4940129.1"
                     /translation="MAWNEPGGGRNNQDPWRGRDGRGGSSDLDDVLKKLQDRFGGMFG
                     GSGDDNSSLGLIGLILGILVIVWGVAGFYRVEQAEQAVVLRFGKYHDMVSAGLHWNPW
                     GIDSVMKVDTSQLNSMPVRATMLTEDQNIVDIDLQVQYRVNDAQAYFLDSSDPIAALR
                     NSVESALRHVVGGSEMDRVLTDGREAIAVEVDKRLQEYLDRYKTGLVVEKINVEDAHP
                     PKDVKDAFDDVIKAKEDEERVKNEAQAYANQVVPEARGKAQRMIEEANAYKSEVVERA
                     AGEAARFEQMYAQYKKAPEVTRRRLYIETMEQVMGNSTKVMVDTEGGNNLLYLPLDKM
                     LQGAAPLVAPVPADMSSTQQSRTDTAKPADTVRGLDRIRQSRDSRWEGR"
     gene            1949..2815
                     /gene="hflC"
                     /locus_tag="VFX11_15735"
     CDS             1949..2815
                     /gene="hflC"
                     /locus_tag="VFX11_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX4940130.1"
                     /translation="MNPRGMTVLVVLALVALLISGTAFTIKQSERGVLLRFGEIVRAD
                     LQAGLHFKVPIMHEARIFDGRVRVIEMRQEEYLTQEKKRLIVDSYVVWRIEDVSKSYT
                     ATGGGQESKVQQLLAPRVNEGLRNQFGERTVYEVVAGEREELVLGLTKTVGEKAREGM
                     GIEVVEIRVKRIELPSNVSGSVYERMRAEREREARGHRSRGNELAEGIRADADRQRTV
                     ILATAYKQAEELRGEGDGLSAAIYANAYQKDSEFYNFYRSLEAYRTVFRDKRDVLVVK
                     PDGEFFQYLKKD"
     gene            2953..4137
                     /locus_tag="VFX11_15740"
     CDS             2953..4137
                     /locus_tag="VFX11_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4940131.1"
                     /translation="MATAERWLLPDGIEEILPPRAARLEQLRRDLLDLYRNWGYEQVF
                     PPLVEYLESLLTGAGNDLALQTFKLTDQLTGRLMGIRADMTPQVARIDAHTLKREGVT
                     RLCYAGHILHAKPESLTASRCLIQIGAEIYGHGAVASDIEVIRLMLETLTASGCDSIH
                     LDLGHVALYRTLAGAAGLSEAQEWQLFEIYQRKAVPELEQFLAAQVRDTGLRAMLREL
                     VSLAGDRSVIGKAREKLGAAPRAVQQALTDLEQIADQLSLSHPGVNLYFDLGELRGYH
                     YHTGPVFSAYVPGMGQAIANGGRYDHIGEVFGRARPATGFSTDLAELVRRSADPVIPE
                     RIFAPAQGDAGLQALVRSLRAAGEIVIQALDDAQTPQSEGCTRQLLQGASGWAVSAVS
                     TS"
     gene            4162..>5391
                     /locus_tag="VFX11_15745"
     CDS             4162..>5391
                     /locus_tag="VFX11_15745"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX4940132.1"
                     /translation="MGKSVVILGTQWGDEGKGKIVDLLTDRVACVVRFQGGHNAGHTL
                     VIDGVRTALHLIPSGILRDNVKCLIGNGVVLAPDALLKEIAMLDEKGVPVRERLRLSP
                     ACPLILPYHVALDKAREVARGKAAIGTTGRGIGPAYEDKVARRGLRLGDIFHRERFAS
                     KLGEVLDYHNFVLKNYYKSETIDFQKTLDDTMLMGEQLKPMVADVAEILHQHRLAGDN
                     ILFEGAQGTLLDIDHGTYPFVTSSNTSAGGTATGSGYGPLYLDYVLGITKAYTTRVGS
                     GPFPTELFDDVGDHLREKGHEYGTTTGRTRRCGWFDSVALRRAIQINSVSGICLTKLD
                     VLDGLDVIRICVGYKDASGKMIESFPVDAADYDGLVPVYEEVPGWKESTFGVKSVDEL
                     PANAISYIKTLEAAVGAP"
BASE COUNT         1059 a         1536 c         1783 g         1013 t
ORIGIN      
        1 catcccgctg gtcaccctgg tgggctacac caatgccggc aaatccacct tgctcaaccg
       61 gctggcgcag gccgacgtgt atgtggcgga ccagttgttc gccacgcttg accccactgt
      121 gcgtcgtatc gaggtgccgg atctgggcgg catcatcctg gcggacaccg tgggtttcat
      181 tcgccacctg ccccacaagc tggtggaagc cttccgcgcc accctggaag aaacccgcga
      241 tgcggatttc ctgctgcacg tggtggacgc ctgcgcggtg gatcgcgagg acaacatttt
      301 ccaggtgcag gaagtgctgg cggaaattgg cgccgacgat gtgccgggag tgatggtgta
      361 caacaagatc gacatgatgg gtgatttccc gccccgcatc gaccgcgacg aggagggcac
      421 cgccactgcc gtgtgggtat ccgccgtcac tggtgcgggg ctcgacctgc tgttgcaggt
      481 gctgaacgaa cggttgggca aaaagatggt ggagggcgtg ttgcgcctgt tgccggagca
      541 gtccgccgtg cgcgcccact gttatgaact caaggcggtg caggacgagt ccgccgacga
      601 cagtggtaat tcattgctgt cgatccgcat gccggtcaag gcctggcagc agatgctgaa
      661 acggttcggt ctgcagggcg acattctgat cgattgaggg tggttgcgaa aaaaacgtcg
      721 atcagaataa ttaaacctac tataacgttc tgagatgcaa taacatttcg aagtttggac
      781 aaatacggag ctattttatg gcctggaacg agcccggcgg cgggcgcaac aatcaggatc
      841 cgtggcgcgg ccgcgatggt cgcggcgggt cttccgatct ggatgacgtg ctcaaaaagt
      901 tgcaggaccg tttcggtggc atgttcggcg gctccggtga cgacaacagc agtctgggtc
      961 tgatcggcct gattctgggc atactcgtga tcgtctgggg tgttgccggt ttttaccggg
     1021 tggagcaggc cgagcaggcc gtggtcctcc gcttcggcaa gtaccatgat atggtttccg
     1081 ccggtctgca ctggaatccc tggggtatcg acagcgtcat gaaggtcgac accagccagc
     1141 tgaattccat gccggtgcgc gccaccatgc tcaccgagga ccagaacatc gtcgacatcg
     1201 acctgcaggt ccaataccgg gtcaacgatg cccaggctta ttttctggat tccagcgatc
     1261 ccattgccgc tttgcgcaac tccgttgaaa gtgccctgcg ccacgtggtg ggcggcagcg
     1321 agatggatcg cgtgctgacg gatggccgcg aagccattgc agtggaagtg gacaaacgcc
     1381 tgcaggaata tctggaccgc tacaagaccg gtctggtggt cgagaagatc aacgtggagg
     1441 acgcccatcc gcccaaggat gtgaaagatg cgtttgacga cgtgatcaag gccaaggaag
     1501 acgaggaacg ggtgaagaac gaagcccagg cttacgccaa ccaggtggtg cccgaggcgc
     1561 gaggcaaggc ccagcgcatg attgaggaag ccaacgccta caaatccgaa gtggtggagc
     1621 gtgccgccgg tgaagcggcc cgtttcgagc agatgtatgc acagtacaag aaggccccgg
     1681 aagtcacccg gcgccggctg tatatcgaaa ccatggagca ggtcatgggc aacagcacca
     1741 aggtgatggt ggataccgaa ggcggcaaca acctgctgta cctgccgctc gacaagatgc
     1801 tgcagggcgc tgcgccgctg gtggcgccgg taccggcgga catgtcgtcc acccagcagt
     1861 cgcgtaccga taccgccaaa ccggcggaca ccgtgcgggg gcttgaccgc atccgccagt
     1921 cccgtgacag tcgttgggag ggtcgctgat gaatccgcgt ggcatgactg tactggtggt
     1981 actggcgctg gtggcgctgc tgatctcggg tactgccttc accatcaagc agagcgagcg
     2041 cggcgtgttg ctgcgctttg gtgagattgt ccgggcggac ctgcaggcgg gcctgcattt
     2101 caaggtgccg atcatgcacg aggcccgtat cttcgatggt cgcgtccggg taattgaaat
     2161 gcgtcaggaa gaatacctga cccaggaaaa gaagcgcctg atcgtcgatt cctacgtggt
     2221 ctggcggatc gaggatgtgt ccaagtccta cactgccact ggcggcggcc aggaaagcaa
     2281 ggtgcagcag ctgctggcac cgcgggtgaa cgaaggcctg cgcaaccagt tcggcgagcg
     2341 taccgtgtat gaagtggtgg ccggcgagcg ggaagagctg gtgctgggcc tgaccaagac
     2401 cgtgggcgag aaagcccgcg aaggcatggg cattgaggtg gtggaaatcc gggtcaagcg
     2461 gatcgagctg ccctccaacg tcagcggctc ggtgtatgag cggatgcggg cggagcggga
     2521 gcgggaagcc cgtggtcacc ggtcccgcgg taacgagctg gctgagggta tccgtgccga
     2581 tgccgaccgt cagcgcaccg taattctggc cactgcctac aagcaggcgg aagagctgcg
     2641 cggtgaaggc gacggtctgt cggcggccat ctacgcgaat gcttaccaga aggattcgga
     2701 gttctacaac ttctaccgca gcctggaagc ctatcgcaca gtgttccgcg acaagcgcga
     2761 cgtgctggtg gtgaagccgg acggcgagtt cttccagtat ctgaagaagg actgacgggt
     2821 ttccgggtcg ggtttcgggc cgtaaaaaac cggaatcgga tctgacctgg accggcaagc
     2881 gtggtatcat aaggaaaccg gggcgctccc ccggtttttt ttcgaccaca gggtttacgc
     2941 agcgatcaca gcatggctac cgcagaacga tggttacttc ctgacggaat cgaggaaata
     3001 ctgcctcccc gggcggcccg gctggagcag ttgcggcgtg atctgctgga tctgtatcgc
     3061 aactggggtt acgaacaggt atttccgccg ctggtggaat atctggaatc cctgctgacc
     3121 ggggccggca atgatctggc cctgcagaca ttcaaactga ctgaccagct taccggccgc
     3181 ctgatgggca tccgcgccga catgacaccc caggttgccc gcatcgacgc gcacaccctg
     3241 aagcgggaag gggtcacccg gctctgctat gctggccata tcctgcacgc caagcccgaa
     3301 agtctcaccg ccagccgctg cctgatccag atcggcgccg aaatttacgg ccacggcgcg
     3361 gtggccagcg atatcgaagt catccgcctg atgctggaaa ccctgaccgc cagtggctgc
     3421 gacagtattc acctggatct gggccacgtg gcgctttacc gcacgctggc cggcgcggcg
     3481 ggtctgagcg aagcgcagga atggcagtta tttgagatct accagcgcaa ggccgtgccg
     3541 gaactggaac agtttctggc ggcgcaggtg cgcgacaccg gcttgcgtgc catgctgcgg
     3601 gaactggtgt ccctggcagg cgaccgcagc gtgattggca aggcgcggga aaagctggga
     3661 gcagcaccca gggccgtgca acaggcgctg acggatctgg agcagattgc cgatcagttg
     3721 agcctgtctc atcccggcgt gaacctgtat ttcgatctgg gcgaactgcg cggctaccac
     3781 taccacaccg gcccggtgtt cagcgcttat gtgccgggca tggggcaggc gatcgccaac
     3841 ggcggccgct acgatcatat tggcgaggta ttcgggcgcg cccggcccgc caccggtttc
     3901 agcaccgatc tggcggaact ggtgcggcgc agcgccgacc ccgtcattcc cgaacgtatt
     3961 ttcgcgccgg cgcagggcga cgcaggcctg caggcgctgg tccgctcgct gcgggccgcc
     4021 ggcgaaattg tcatccaggc gctggatgat gcacagacac cccagtccga gggttgcacg
     4081 cggcagttgc tgcagggtgc ctccggctgg gccgtatccg cagtttccac ttcttaattt
     4141 ttctggcaga gacgaaaggc aatgggcaag agtgtcgtta ttctgggtac ccaatggggc
     4201 gatgaaggca agggcaagat cgttgacctg ctgacggatc gcgtggcctg tgtggtgcgc
     4261 ttccagggcg gtcataacgc cggtcacaca ctggtgatcg acggcgtcag aaccgcgctg
     4321 cacctgattc cctccggcat tctgcgcgac aacgtgaagt gcctgatcgg caacggcgtg
     4381 gtgctggcac cggacgcgct gctgaaagaa attgccatgc tggacgaaaa gggtgtgccg
     4441 gtgcgcgagc gcctgcgcct gagcccggcc tgtccgctga tcctgcctta tcacgtggcg
     4501 ctggacaaag cgcgcgaagt ggcgcggggc aaggcggcga ttggtaccac cggtcgcggt
     4561 atcggtccgg cctatgaaga caaggtggcg cgtcgtggcc tgcgtctggg tgacatattc
     4621 caccgcgagc gtttcgcctc caagctgggc gaggttctgg attaccacaa cttcgtgctg
     4681 aagaattact acaagtccga gacgatcgat ttccagaaaa ccctcgacga caccatgctg
     4741 atgggtgagc agctgaagcc gatggtggcg gacgtggcgg agatcctgca tcagcatcgt
     4801 ctggcgggtg acaacatcct gttcgaaggc gcgcagggca ctctgttgga tattgatcac
     4861 ggtacctacc cgttcgtgac atcgtccaac accagcgctg gcggcactgc cacgggttcc
     4921 ggttacggcc cgctgtatct ggattatgtg ctgggcatca ccaaggccta caccacccgc
     4981 gtcggttccg gcccgttccc caccgagctg tttgatgatg tgggtgatca tctgcgggaa
     5041 aaaggccacg aatacggcac caccaccggc cgcacccgcc gctgtggctg gttcgattca
     5101 gtggcgctgc gccgtgccat ccagatcaac tcggtgagcg gcatctgcct gaccaagctg
     5161 gatgtgctgg atggtctcga tgtgatccgc atctgcgtgg gttacaagga tgcgtccggc
     5221 aagatgatcg aaagcttccc ggtggatgcg gcggattacg acggtctggt gccggtgtat
     5281 gaagaagtgc cgggctggaa ggaatccact ttcggtgtga agagcgtgga cgagttgccg
     5341 gccaatgcca tctcatacat caagacgctg gaagcggcgg tgggcgcgcc c
//
LOCUS       DASUEG010000835         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_335710, whole genome shotgun sequence.
ACCESSION   DASUEG010000835 DASUEG010000000
VERSION     DASUEG010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335710"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFX11_15750"
     CDS             <1..180
                     /locus_tag="VFX11_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX4940133.1"
                     /translation="VALNAELRVPTPEAFGIDRATFFNLTSTMAEQALASGSPGNNPR
                     VPTVDEMVKLYEQLW"
     gene            complement(212..1147)
                     /locus_tag="VFX11_15755"
     CDS             complement(212..1147)
                     /locus_tag="VFX11_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEX4940134.1"
                     /translation="MRKFTVLAILALAAVLLFFWLPAEVDDTGQNPGTDTATPAPGGT
                     AVPAPGERVVTLLDIPIRISASGDLVQDTRIKQLFDRFAAEHTEEPVDGWKQSILQSY
                     ADQLPAPALAQLQDLLNRYVEFNLALQLLPLEGAPDLNGVLARVRELREHYLGKSASD
                     SIYADWSALENFTHQYLDIMTRNRDPAQSEAELEKLARALPEPVQERALSMIRHNDEE
                     MSVQRIAKVDPVAYGRMLQEQAAIALIETQLLFDEPSPEFMERYEEYAQERQSLLKEN
                     VDLRLQQDQLKDLRAKYFSGADQLRVETLDRAEAF"
     gene            complement(1216..2490)
                     /locus_tag="VFX11_15760"
     CDS             complement(1216..2490)
                     /locus_tag="VFX11_15760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940135.1"
                     /translation="MTRKRLPDMRNLPVSDPALQREISAVLAGGFRFLRFPDFLEPYF
                     SGHYRLLAVNTLMANTFYLTTLYLLMGVLAFSQYSSEQLQLFPLGYFITGGCLVVTLL
                     FPRLQRFDRWFNWYTGLSTVGALVALMTAAASVEDRDAMTSAHATSIYAVIIVYAMSK
                     MRFYMTVIWCALAGVLHLVLLDVLELPRSFHNFQAYFIAANLIGMGIAYVIEHRERVM
                     FLQALLLDIDKAQQRLLYANLEKLSREDPLTGLANRRYFDERLNMEWNRCQREKLPLS
                     VILLDVDFFKQYNDLYGHQAGDHCLTLIARALRKEASRPAEVVGRYGGEEFIILYPNV
                     DANQIRNTLVRIQQRLLALAVLHEGSRIAKVVTASLGAATAYPVRNLNPEKLVSAADQ
                     MLYKSKEAGRNCWFNTQLSPQEPQQQDLINLS"
BASE COUNT          525 a          770 c          806 g          494 t
ORIGIN      
        1 gtggcgctca atgcggagct gcgcgtgccg acaccggaag cgttcggcat cgatcgggca
       61 accttcttca acctgacctc caccatggcg gaacaggcgc tggcttcagg atcgccgggc
      121 aacaatcccc gtgtgcccac ggtggacgag atggtgaagc tgtacgaaca gctttggtga
      181 ctaacagtat cggtgacagg ggcgcagggg atcagaacgc ctccgcccga tccagcgtct
      241 ccacccgcag ctgatcagcg ccggaaaaat atttcgcccg cagatccttc agctggtcct
      301 gctgcaggcg caggtccaca ttttctttca gcagcgattg ccgctcctgt gcgtactctt
      361 cataacgttc catgaattcc ggtgacggct cgtcgaacag cagctgggtt tcgatcagcg
      421 cgatcgccgc ctgttcctgc agcatgcggc cgtaagcaac cgggtccacc ttggcgatgc
      481 gctgcaccga catttcctca tcgttatggc ggatcatgct gagggcacgc tcctgcaccg
      541 gctcgggcag cgcgcgggcc agtttctcca gctccgcttc cgactgtgcg ggatcgcggt
      601 tgcgggtcat gatatccaga tactggtggg tgaagttttc cagcgcgctc cagtcggcat
      661 aaatgctgtc ggatgcgctc ttgcccagat agtgctcgcg caattcccgc acccgtgcca
      721 gtacgccatt caggtccggt gcgccttcca gcggcagcag ttgcagcgcc agattgaatt
      781 ccacatagcg gttgagcaga tcctgcagtt gcgccagggc gggggcgggc aactggtcgg
      841 cgtatgactg cagaatggat tgcttccagc cgtccaccgg ttcttcggta tgctcggcgg
      901 cgaagcggtc gaacagttgc ttgatgcggg tgtcctgcac cagatcgccc gacgcgctga
      961 tgcggatcgg aatatccagc agcgtgacca cccgctcgcc aggcgcgggt acggcagtgc
     1021 caccgggtgc gggcgttgcc gtgtcggtgc cgggattctg gccggtgtcg tcgacctcgg
     1081 ccggcagcca gaaaaacaga aggaccgcag cgagcgccag aatcgccagg accgtgaatt
     1141 tgcgcattga atgtctcgaa cagaataggc cggaaggggt aattgccagt gcgcaatata
     1201 ccgctcacgc agggtttatg aaagattgat cagatcctgc tgctgcggtt cctgtgggga
     1261 cagctgcgtg ttgaaccagc agttgcggcc ggcttccttc gacttgtaca acatctggtc
     1321 cgcggcggac accagtttct cggggttcag atttcgcacg gggtaggcgg tggccgcgcc
     1381 caggctggca gtgacgacct tggcgatgcg cgagccctca tgcaaaaccg ccagagccag
     1441 cagacgctgc tgaatccgta ccagcgtatt gcggatctgg ttggcgtcca cgttgggata
     1501 aagaatgatg aattcctcgc cgccgtagcg ccccaccact tctgccgggc ggctggcttc
     1561 cttgcgcagc gcccgtgcaa tcagcgtcag gcaatgatcg ccggcctgat ggccatacag
     1621 atcgttgtac tgcttgaaga agtccacatc cagcaggatc accgacagcg gcagtttctc
     1681 gcgctggcag cggttccatt ccatgttcag gcgctcgtcg aaatagcgcc ggttggccag
     1741 cccggtcagg ggatcttccc gcgacagctt ttccagattg gcatacagca ggcgttgctg
     1801 ggctttgtcg atatccagca gcagcgcctg caggaacatc acccgctcgc ggtgctcaat
     1861 gacataggcg atgcccatgc cgatcaggtt ggcggcaatg aagtaggcct ggaaattgtg
     1921 aaacgaccgg ggcagctcca gtacatccag caataccaga tgcagcacgc ctgccagcgc
     1981 acaccagatt accgtcatgt agaagcgcat tttcgacatc gcgtagacga tgatcaccgc
     2041 atagatcgag gtggcatggg cgctggtcat cgcatcccga tcctccaccg aggccgccgc
     2101 cgtcatcagc gccaccagcg cgcccaccgt gctgaggccg gtgtaccagt tgaaccagcg
     2161 atcgaagcgt tgcaggcggg gaaacagcag ggtgaccacg aggcagccgc cggtgatgaa
     2221 atagcccagc ggaaacagct gcagctgttc gctggagtac tggctgaagg ccagaacccc
     2281 catcaacagg tagagcgtcg tcagatagaa ggtattggcc atcagggtgt tcacggccag
     2341 caggcggtag tggccgctga aatagggctc cagaaaatcc gggaaacgca gaaaccggaa
     2401 gccgccagcc agcacggcgc tgatttcccg ctgcaaggca ggatcactca ccggcaggtt
     2461 gcgcatgtcg ggcaggcgtt tacgggtcat tggagtccgg gaagggggct taccataggt
     2521 ttagcaaagt cccggcggtc gcgccctggt gtagcctata aaaaaaatca aaaaaggccg
     2581 acctggcgga agtag
//
LOCUS       DASUEG010000836         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1667905, whole genome shotgun sequence.
ACCESSION   DASUEG010000836 DASUEG010000000
VERSION     DASUEG010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667905"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFX11_15765"
     CDS             complement(<1..252)
                     /locus_tag="VFX11_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940136.1"
                     /translation="MVGRFRSATKHLLGLSVMLPLAFAMNAAATEQVCPVTSPANNTA
                     EVVTEQGPDYGDINRVLLLNDDEQRRTALRHLLHDISTKT"
     gene            372..2006
                     /locus_tag="VFX11_15770"
     CDS             372..2006
                     /locus_tag="VFX11_15770"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX4940137.1"
                     /translation="MIARMLVTALVLGSTLARADATAERLLKQDIDRREQEQRERRWD
                     EPLTTSPSPQAVSPSAPPSGDSGPCFSISEIRVVQGHVLPAKPLQRLIASYQGRCLNA
                     ADLQALQQGMNSLALARGLVTTRVVIPEQNLTSGMLVLEVWPGHLEGFTVNSPYRMDV
                     EMALPLQEGDLLNLRALEQAIDSLNRLESFQAGVELEPGERPGGSVAAFTLNRQKPWH
                     LMAAWDSEAMEQNPNNTVRVSLTLDNPLRMADRLIIGSHANLQDAEVDNAKGSSIDYD
                     LPMGWWRFSAGADQFDYRNPITVGLTTFVSSGRSQSLRLELARTFWRDGKHRLNVALQ
                     GKQRVNDNFIDDVTVGVSTSRLKALGLRADVSRVAAPWVMDASLGLDHGDTRLKAHPS
                     PVDDDYSRLNLNTRVQYHWPRSSLSWSLNGQWSDAVLLPSEQLALAGQVRGYAPLSLN
                     AATALASRLEWARPVLFDRWGFTSVRPQIGAEFAWAPHASGNPGAERLSAVTTGLTVP
                     WKQVLTQLQIAAPLDTGSTQTPPDDWQLDASVSVKW"
     gene            2006..>3707
                     /locus_tag="VFX11_15775"
     CDS             2006..>3707
                     /locus_tag="VFX11_15775"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX4940138.1"
                     /translation="MKMVKQGLTITLIALQVWQPVLAGVVASNPQITQDQAANGVPVV
                     NIATPGQSGISHNTYTRFDVDRQGLILNNSGQPVNTQLGGFIPGNANLHSGSARLILN
                     EVSGNLPSQLKGYMEIAGQKAEMIIANPAGITCDGCGFINTSRGTLTTGKPLFGSDGS
                     LNGFRVEDGMLAIDGLGLNASNTDRLNLYSRALVLNAELHARDVNVMTGTNDINVVNG
                     VVTPTASGSTTPTFSIDSSSLGGMYANRIRLVGTEAGVGMRLAAPVAAMNGNLEITSN
                     GDVRLVRASATQRVDVRAEGNVAIDSDGAGDRDGISSGAGTTLRATGAVTLASGADIT
                     GTVIGLMADSVDTENDSDIAARGALVIDAANMQLAGTIASRQTITLTGNTLANQGQMA
                     ANTGLTITASSLDNSGRISSAGGNGRLTVAGELRNDGGIIQQAGTLDITAAGVSSING
                     AIAANGNLSIAATNTSVDNTGGLLQGNTGIFVRATGLVNSSGEVVSNGDIHLLLDDGA
                     IDNRNGLIETLGNIDLLAKGLLQNSGGRFRSGADMTFTLDAFDRALAGGSFAADGLWK
                     I"
BASE COUNT          758 a         1044 c         1160 g          745 t
ORIGIN      
        1 tgtttttgtg gagatgtcgt ggagcagatg acgtaaggcc gtgcgacgtt gctcatcgtc
       61 attcagcagc aatacacggt tgatgtcacc gtagtccggt ccctgttccg taacgacctc
      121 tgccgtattg ttggcaggtg aggtgacggg gcacacctgc tcggttgctg cggcattcat
      181 cgcaaatgcg agcggcaaca ttacggaaag accgagcaag tgcttggtcg ctgaacggaa
      241 acggccaacc atggcgtgaa ctcctgcacc tgtcccgggg agggggccgg gagcgcggcg
      301 tagtctccat tatttgtaat gacagggcca gcatcctaga atgcgcgccg cacaggaatg
      361 tggatgcgta gatgattgca cggatgctgg ttacggctct ggtgctgggc agtacgcttg
      421 cccgggcaga tgccactgcg gaacgcctgc tcaagcagga catcgaccga cgggagcagg
      481 agcagcggga acggcgctgg gatgagccgt tgacgacgag tccgtcgccg caagcagtgt
      541 ctccctccgc accgccctcg ggtgattccg gcccctgctt ttccatttcc gaaattcgcg
      601 tagtgcaagg gcacgtgctg cccgccaaac ccctccagcg cttgattgct tcctaccaag
      661 gccgttgcct gaacgctgcc gatctgcagg ccttgcagca gggcatgaac agtcttgcac
      721 tggcgcgtgg cctggtgacc acacgtgtag tgattccgga acagaacctt accagcggca
      781 tgctggtgct cgaagtgtgg ccgggccatc tggaaggctt taccgtcaat tccccctacc
      841 gcatggatgt ggaaatggcg ttgccgctac aggaaggcga tctgctcaac ctgcgtgcgc
      901 tggaacaagc catcgacagc ttgaatcgcc tggagtcctt tcaggcaggc gtcgaactgg
      961 agccgggcga gcggccagga ggctcagtcg ccgcgtttac cctcaatcgc cagaagccct
     1021 ggcatctcat ggccgcgtgg gacagcgagg ccatggagca gaatcccaac aacacagtac
     1081 gcgtcagtct cacgcttgat aatccgttgc ggatggcgga tcgcctgatc atcggcagcc
     1141 atgccaacct gcaggatgcg gaagtcgaca atgcgaaagg cagcagcatt gattacgacc
     1201 tgccgatggg ttggtggcgt ttcagtgcag gtgcggacca gttcgattat cgcaatccga
     1261 ttacggtagg cctgacgact tttgtctcca gcggtcgcag ccagtcgctc cgactggagc
     1321 tggcgcgcac gttctggcgc gacggcaagc atcgcctgaa tgtcgcgctg cagggcaagc
     1381 agcgtgtgaa cgacaacttc attgatgatg tcaccgtggg cgtcagtacc agtcgcctca
     1441 aggccctggg tctgcgcgcc gatgtgtcgc gcgtggcggc gccctgggtc atggatgcca
     1501 gcctggggct ggatcatggt gacacgcgcc tcaaggcgca cccctctcca gtggatgatg
     1561 actactcccg tctcaatctg aacacccgtg tgcagtacca ctggccgcgc tccagtttga
     1621 gctggtcgct gaacgggcaa tggagtgatg ccgtgctttt gccgagcgag caactggcgc
     1681 tggccgggca agtacgtggt tatgcgccgc tcagcctgaa tgccgcgact gccttggcca
     1741 gtcgactgga atgggcgcgc ccggtgctct ttgatcgctg gggatttacc tcggtacgtc
     1801 cgcagatcgg cgccgagttc gcatgggcgc ctcatgccag tggtaatccg ggtgccgagc
     1861 gtctgagcgc cgtgacgacc ggacttaccg tgccgtggaa gcaggtactg acccagttgc
     1921 agattgccgc cccgctcgat acgggcagca cgcaaacccc tcctgatgac tggcaactgg
     1981 acgccagtgt tagcgtgaaa tggtgatgaa gatggtcaag caaggactga cgataacgct
     2041 gattgcactg caagtatggc aaccggtgct ggcaggcgtg gtggccagca atccgcagat
     2101 cacgcaggac caggccgcca acggcgtgcc tgttgtcaac atcgctacac cgggccagag
     2161 cggcatttcg cataacacct atacgcgctt cgatgttgat cgtcaggggc tcatcctcaa
     2221 caacagcggc cagccggtaa acacccagct gggcggcttc atccccggta acgccaatct
     2281 gcacagcggt agcgcgcgac tcatcctcaa tgaagtcagc ggcaacctgc ccagccagct
     2341 caagggttac atggagatcg ccggccagaa ggccgagatg attatcgcca atccggccgg
     2401 cattacctgt gacggttgcg gcttcatcaa caccagtcgc ggcacgctta ctaccggcaa
     2461 gccactgttt ggcagtgacg gcagtctcaa tgggttccgg gtggaagacg gcatgctggc
     2521 gatcgatggt cttggcctta atgccagcaa taccgaccgg ctcaatctgt acagccgtgc
     2581 gcttgtgctg aatgccgaat tgcacgcgcg cgatgtgaat gtgatgacgg gcaccaatga
     2641 catcaatgtt gttaatgggg tcgtcacacc aactgcctct ggctcaacta cgccgacttt
     2701 ctccatcgat agcagcagcc tcggtggcat gtatgccaac cgcattcgcc tggtaggcac
     2761 tgaggcgggc gttggcatgc gtctggccgc tcccgtggcc gccatgaacg gcaatctgga
     2821 aatcaccagc aatggtgatg tgcggctggt gcgggccagt gcgacgcaac gtgttgatgt
     2881 tcgtgcggaa gggaatgttg ccatcgacag tgatggtgca ggtgaccggg acggcatcag
     2941 cagtggtgcg ggtacaacac tgagggcaac tggtgctgtc acactggcat cgggtgccga
     3001 catcacgggt accgttatcg gcctgatggc ggacagcgtt gatacggaaa atgacagtga
     3061 cattgctgcc cgtggtgctt tggtgattga tgcagccaac atgcagttgg ccggcacgat
     3121 cgcaagtagg cagacgatta ccctgaccgg caataccctc gccaatcagg gccagatggc
     3181 ggctaacacc ggactcacca ttacggcttc gtcactcgac aacagtggac gcatcagtag
     3241 tgcgggtggt aatggtcggt tgacggttgc gggtgagttg cgcaatgacg gtggaataat
     3301 ccagcaggct gggacactgg atatcacggc ggctggagtc agctccatca atggagcaat
     3361 tgccgccaac ggcaacctct caatcgcggc gaccaataca tctgtcgaca atactggcgg
     3421 gctgttgcag ggcaatacag gtatcttcgt cagggcgacg gggctggtga attcgtccgg
     3481 agaagtggtc agtaatggcg acatccatct actgctagat gatggcgcca ttgataaccg
     3541 gaacggcctg attgaaacgc tgggaaatat tgatctgctg gccaaaggac ttctgcagaa
     3601 ttcgggcgga cgcttccgct caggtgcaga tatgactttc acccttgatg cgtttgatcg
     3661 cgcgcttgcg ggaggaagct ttgcagcaga tggactttgg aaaatcg
//
LOCUS       DASUEG010000837         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1260672, whole genome shotgun sequence.
ACCESSION   DASUEG010000837 DASUEG010000000
VERSION     DASUEG010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260672"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..540
                     /locus_tag="VFX11_15780"
     CDS             214..540
                     /locus_tag="VFX11_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX4940139.1"
                     /translation="MENILNDGHQRKKRHTRACFKDVATQELNMIAEKIRAILAQHAR
                     LTTDINTLSDASDLYDAGLTSLTTVNLMLAIEDQFDIEFPDHMLGRKTFGSIQALCEA
                     VEELVG"
     gene            576..1763
                     /locus_tag="VFX11_15785"
     CDS             576..1763
                     /locus_tag="VFX11_15785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940140.1"
                     /translation="MHTASTPQLHPDLDKLLERIHTIGREIIAPNADDVDHDARFPHE
                     AFAALKAEKLLSSYIPVELGGMGLSISDQSKICEALAHYCASSAMIYAMHQIQVACIV
                     HHTLQDDSFRAYAREIVEHQLLIASATTEMGIGGDVRSSICAVSIEGDTFTLDKQAPV
                     ISYGATADAILVTCRRSPDAGRNDQSVVLVRKQDCTLEEISGWDTMGFRGTCSNGYHL
                     RSRGAARQVFPGPYADIHRKTQQPFSHIVWSSLWLGLAQSAMNKARASVRAEARKNPG
                     TLPPTALRLAELDAVLFSMRAGVQQCVADYHQRLLDTDDAAFSDYGFNLRVSNLKIRT
                     SELVIEVLTKAMMICGIASYRNDSKLSLNRQLRDAYGAALMVNNDRILGQSSAIQIMQ
                     KGD"
     gene            1770..>2349
                     /locus_tag="VFX11_15790"
     CDS             1770..>2349
                     /locus_tag="VFX11_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940141.1"
                     /translation="MSTTDALQESYQRYLNQLFDAKLLIPSGVKGVYGRSGTFENVID
                     QFEQYVTRQTRDMKPEVVRFPPVFNRQHYAQTDHIYNFPDLMGSVHSFMGREPEHAEL
                     LHKFQCCGDWAHDLDATEVMLAPAACYPLYPSATGTLPEGGRLVDLKSFVFRHEPSDD
                     PARMQSFRMREFVRLGTPEQALAHRDFWLENAQ"
BASE COUNT          512 a          763 c          624 g          450 t
ORIGIN      
        1 cgtaagctga tactattgtg cgctccaaat ggggtggtca cattcccgtc ggccagtcat
       61 ctgcctgtca cactcgtgca cgcacatttc tggcatctat agttgataac acagctcaac
      121 tttatcgtgc tccaggatgg ggtttcccgc ctgccggaaa gcactgttgg gaagcagcca
      181 tgctgctgga aatgacacag ccacccggtt catttggaaa acattctcaa tgacggccac
      241 caacgtaaaa agcggcacac ccgtgcatgc ttcaaagatg tagcgacaca ggaactgaat
      301 atgattgcgg aaaaaatcag ggccatcctt gcccagcatg cccgtctcac gactgacatc
      361 aacaccctga gcgatgcaag cgacttgtat gacgccggcc tgacctccct gaccaccgtc
      421 aacctgatgc tggcgatcga agatcaattc gatatcgaat ttcccgatca catgctcggc
      481 cggaaaacct ttggcagcat tcaggctctg tgcgaggccg tggaagaatt ggtgggctaa
      541 tcaccaccca acgttaggcc cttggagata aaggcatgca caccgcgtca accccccagc
      601 tccaccctga tctcgacaaa ttactggaac ggattcatac catcggccgc gaaatcatcg
      661 cccccaatgc cgatgacgtc gatcacgacg cacgttttcc gcacgaggcc ttcgctgcgc
      721 tgaaagccga gaagctgctg agctcctaca tacccgtcga actcggcggc atggggctgt
      781 ccatttccga ccagtccaag atctgcgaag cactggccca ttactgcgcc tccagtgcca
      841 tgatctacgc catgcaccag atccaggtcg cctgcatcgt gcaccacacc ctgcaggacg
      901 attcgttccg cgcctacgcc cgcgaaatcg tcgagcacca gttgctgatt gcatccgcca
      961 ctaccgaaat gggcataggc ggcgatgtgc gttcgagcat ctgcgccgtc agcatcgaag
     1021 gcgacacttt cacgctggac aagcaggcac cggtgatttc ctacggcgcc actgccgacg
     1081 ccatcctggt cacctgccgc cgctcgccgg acgcaggccg caacgaccag tccgtggtac
     1141 tggtgcggaa acaggattgc acactggaag aaatctccgg ctgggacacc atgggtttcc
     1201 gtggcacctg cagcaatggc taccacctgc gttcacgcgg cgcggccagg caggtcttcc
     1261 ccggccccta cgccgacatc caccgcaaga cgcagcaacc cttttcgcac atcgtgtgga
     1321 gttcgctgtg gctgggcctg gcacaaagcg cgatgaacaa ggcccgcgca tcagtgcgtg
     1381 ccgaagcgcg caagaatccc ggcacactgc cgccgacggc gctgcgcctg gcggaactgg
     1441 acgccgtact gttttccatg cgcgccggcg tgcagcaatg cgttgccgat tatcaccagc
     1501 ggctgctgga taccgatgac gccgcgtttt ccgattacgg cttcaacctg cgcgtgagca
     1561 acctcaagat ccgcacctcc gaactggtga tcgaagtcct caccaaggcg atgatgatct
     1621 gcggcatcgc cagctaccgc aacgattcca aactgagcct gaaccgccag cttcgcgatg
     1681 cttacggcgc agcactcatg gtaaacaacg atcgcatcct cggtcagagt tccgcaatcc
     1741 agatcatgca aaagggagat tgatttgcca tgtccaccac tgacgcgctg caagaatcct
     1801 atcaacgcta tctgaaccag ctgttcgatg cgaagctgct catcccgtcc ggcgtcaaag
     1861 gcgtgtatgg gcgctccggc accttcgaga acgtcatcga ccagttcgaa cagtatgtga
     1921 cacggcagac ccgcgacatg aaaccggaag tcgtgcgctt cccgccggtg ttcaaccgcc
     1981 agcactacgc ccaaaccgac cacatctaca attttcccga tctgatgggc tccgtgcaca
     2041 gcttcatggg ccgcgagccg gaacatgccg agctgctgca caaattccag tgctgcggtg
     2101 actgggccca cgatctggat gccaccgaag tcatgctggc accggcggcc tgctatccgc
     2161 tgtatccgtc ggctactggt accctgccgg aaggcggccg tctggtggat ctgaaatcct
     2221 ttgtgttccg ccacgaaccg tcggacgatc cggcgcgcat gcaatcgttc cgcatgcggg
     2281 agttcgtgcg cctcggcaca ccggagcagg cactggcaca ccgcgatttc tggctcgaga
     2341 atgcgcagc
//
LOCUS       DASUEG010000838         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1469115, whole genome shotgun sequence.
ACCESSION   DASUEG010000838 DASUEG010000000
VERSION     DASUEG010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469115"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2167)
                     /locus_tag="VFX11_15795"
     CDS             complement(<1..>2167)
                     /locus_tag="VFX11_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4940142.1"
                     /translation="QEIQTQLVEWNQTARTYDRELCVHALIHQASQRHANGTAVRCGD
                     EALTHAELDRLSNRVAHQLLANGVGPGDLVGVALDRNLHLMTAILGILKAGAAYVPID
                     AAYPEERIRYILEQARIRVLVTRQHLTPNLPSDCEWLLLDEAVQASAVATSLPTQVSI
                     SSDAWLYVIFTSGSTGKPKGTGAYHRSEVNLLEWYCDEFSLQPSDRILLLSAIGFDLT
                     QKNLFAPLASGATLVIPDFQEYDPARIIDLIEKENITWINCAPSAFYPLVDNEQDWPK
                     LKSLRQVFLGGEPINLARLSAWLRQTSCELVNSYGPTECADIAAYRRIELDADLNAAV
                     LPIGRPNYNVQLYVLGEHQELLPVGAVGELCIGGDGVGPGYLHNPELTVQVFVANPQV
                     TGQPLMYRTGDRVRYRKNGVVEYLGRRDHQIKLRGYRVEAGEIQNLLNQHANVKESLV
                     DVLKSDAGTDQLVAWIVCRELPSDQAAFTHELKQRAEQFLPRFMVPDAWLLLDVFPLT
                     PNGKVDRKALPKPQWQQRTTPFVDPRNETEVALVQIWQDVLRVDRVGVTDNFFELGGH
                     SLLATQVASRVRRQLGVTFQIRDLMLAPTVEAMATKIARSARELDEPAITAVDRSQRI
                     PLSFAQQRLWLLDRIEPGSVAYNVPSVIRIRGALDVGALETALGKVFNRHEGLRAIFL
                     EDEQGPYQQILPPQDWKLPLTQVDDDGLTRQDVELKRLAA"
BASE COUNT          432 a          673 c          634 g          428 t
ORIGIN      
        1 tggcggcgag gcgtttcaat tccacgtcct ggcgggtcaa gccatcatcg tccacctgtg
       61 tcagcggcaa tttccagtcc tgcggcggca ggatctgttg atacgggccc tgctcgtctt
      121 ccagaaaaat ggcgcgcagt ccttcgtgac ggttgaatac cttgcccagc gcagtttcaa
      181 gcgcccccac atccaatgcg ccgcgaatgc ggatcaccga tggcacgttg taggcaacag
      241 agccgggctc gatccggtcc agcagccaca gacgctgctg ggcaaacgac agcggaatgc
      301 gttgactgcg atccaccgcc gtaatggccg gctcgtccag ctcccgcgcg ctgcgcgcaa
      361 tttttgtcgc catggcctcg acggtgggtg cgagcatcag atcgcgaatc tggaaggtga
      421 cgcccagttg ccggcgcacg cgcgatgcta cctgcgttgc cagcagggaa tgcccgccca
      481 gctcgaagaa attatcggtg acgccgacac ggtccacccg caacacgtcc tgccagatct
      541 gcaccagcgc cacttcggtt tcattgcgcg gatcgacaaa cggcgtggtg cgctgctgcc
      601 actgcggttt tggcagcgcc ttgcgatcga ctttgccgtt gggcgtcagc gggaaaacgt
      661 ccagcaacag ccaggcatcc ggcaccatga aacgtggcag gaactgttcg gcgcgctgtt
      721 tcagttcgtg cgtaaaagcg gcctgatcag acggcagttc gcggcagacg atccaggcca
      781 ccagctggtc ggtacccgca tcggatttca gcacatccac cagcgattct ttcacgttgg
      841 catgctggtt cagcagattc tggatttcac cggcctcaac ccggtagccg cgcaacttga
      901 tctggtgatc gcgacgcccc agatattcaa cgacgccatt tttgcgataa cgcacacggt
      961 cgccggtgcg gtacatcagc ggctggcctg tcacctgcgg gtttgccaca aacacctgca
     1021 ccgtcagttc cggattatgc agataaccgg ggcccacgcc gtcaccgccg atacacaact
     1081 cgccgacggc acccacaggc agcagttcct ggtgttcgcc caacacatac agctgcacgt
     1141 tgtagttggg acggccgatc ggcagcaccg ccgcattcag atccgcatcc agttcaatac
     1201 ggcgataagc ggcgatatcc gcgcactcgg taggaccgta actgttcacc agttcgcagg
     1261 acgtctgccg cagccaggcc gacagacgcg ccaagttgat cggctcgccg cccagaaaca
     1321 cctgccgcag tgatttgagt ttcggccagt cctgttcatt atccaccagc ggataaaacg
     1381 cgctgggtgc gcagttgatc caggtgatgt tttctttttc gatcagatcg atgatgcggg
     1441 ccggatcata ttcctgaaaa tccggaatta ccagcgtggc gccggacgcc agaggcgcga
     1501 acaggttttt ctgcgtcagg tcgaaaccga tggcgctcag cagcagaatg cggtcggacg
     1561 gttgcaggga gaattcgtcg caataccatt ccaacaggtt cacttcggaa cggtgatacg
     1621 cgccggtgcc tttgggtttt ccggtggagc cgctggtgaa aatcacatac agccaggcgt
     1681 cgctggagat actcacctgc gtcggcaacg acgtcgccac tgcactcgcc tgcaccgctt
     1741 cgtccagcaa cagccattcg caatcggacg gcagattagg tgtcagatgc tggcgcgtga
     1801 ccagcacgcg gatgcgggcc tgctccagaa tgtaacggat gcgttcttcc ggatatgcgg
     1861 catcgatggg cacataggcg gcgccggcct tgagaatgcc gagaatcgcg gtcatcaaat
     1921 gcagattgcg gtccagcgct acgcccacca gatcgccggg gccgacaccg ttggccagca
     1981 attgatgggc aacgcgattt gacagccgat ccagctcggc atgcgtcagc gcctcatctc
     2041 cacaacgcac tgcggtgccg ttggcgtgac gctgtgacgc ctgatgaatc agcgcgtgca
     2101 cgcagagttc gcgatcatag gttcgtgccg tctggttcca ttccaccagc tgggtctgga
     2161 tttcctg
//
LOCUS       DASUEG010000839         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1434853, whole genome shotgun sequence.
ACCESSION   DASUEG010000839 DASUEG010000000
VERSION     DASUEG010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434853"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFX11_15800"
     CDS             complement(<1..792)
                     /locus_tag="VFX11_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4940143.1"
                     /translation="MDLLGTSIGSWRLASYAQPDPLTAIERFEAAYFEQHYSTKPDMS
                     EISRECGRILDALLGKEHSAIVTNPHRRLHTIASRCRSLLLNAGGEPGKWQLGAAALM
                     NGVGRRHLGHFFERVLFAPQHSQLPFRETLFMQENAMLDSTNLRPALMATASIPVIMQ
                     AVQDIPGTSGGSCVDGGITDYHFDTPLPYDSGIVLYLHFSPRLIPGWFDKLLPWRKPQ
                     PASLENVLLITPSRDFIAALPHGRIPDRNDFLTMRNDERIASWRRV"
     gene            complement(969..1316)
                     /locus_tag="VFX11_15805"
     CDS             complement(969..1316)
                     /locus_tag="VFX11_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX4940144.1"
                     /translation="MITIYGIKNCDTMKKAFAWLNSQEVAFTFHDYKTSGIDAATLAR
                     WESSLGWEALLNRRGTTWRKLDASTQANINRDLALLLMQQQPSLIKRPVLAFGPRLLC
                     GFDEKVWQEALGK"
     gene            complement(1375..2196)
                     /gene="dapD"
                     /locus_tag="VFX11_15810"
     CDS             complement(1375..2196)
                     /gene="dapD"
                     /locus_tag="VFX11_15810"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX4940145.1"
                     /translation="MSLQQLIENAFDRRAEFSPANTPSDVRTAVNEALAALDAGKLRV
                     AEKTDSGWIVNQWLKKAVLLSFRLNDNAPIAGGGGMQFFDKVPTKFADWTPEQFAAAG
                     VRVVPPAVARKGSYIARNVVLMPSYVNIGAYVDEGSMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVLSMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVPGSLPSKDGKYSLYAAIIVKKVDAQTRSKVGINELLRVD"
     gene            complement(2277..3467)
                     /gene="dapC"
                     /locus_tag="VFX11_15815"
     CDS             complement(2277..3467)
                     /gene="dapC"
                     /locus_tag="VFX11_15815"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX4940146.1"
                     /translation="MNPELARLQAYPFERLAQLFKGLSPAADLSPISLSIGEPKHASP
                     EFVKQVLTDNLSGLSVYPTTKGLPALREAIASWLQKRFHLQKVDAESEILPVMGTREA
                     LFSFTQAAIDRSHKALVLMPAPFYQIYEGAAYLAGAEPYYLPCSADTGFIPDYDAIPD
                     SIWANTQMLFVCSPGNPTGAVTSRETLSKLIALADKHNFIIVSDECYSEIYFDEKHPP
                     VGLLQVCAEIGRHDYARCIVFHSLSKRSNLPGLRSGFVAGDAKLLKPYLLYRTYHGAA
                     MPVQNQQASVAAWSDEEHVIQNRTLYRQKFEEFRAIMAPVYPLTMPDAGFYFWMRTPI
                     DDDVFAQRLYQEKNVTVLPGRFLGRDINDCNPGASHVRLALVASPDECAEAARRLRDF
                     MKSL"
     gene            complement(3486..>3846)
                     /locus_tag="VFX11_15820"
     CDS             complement(3486..>3846)
                     /locus_tag="VFX11_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX4940147.1"
                     /translation="DTLVQTLSNPERFPEIVQRRMPRQLKHFDVKTTVTISNDIVNQR
                     TILEVMTLDRPGLLARIGRIFMESGVVLQNARIATLGERAEDVFFITDKQQHPISDPA
                     VCEQLASRLREQLDQLN"
BASE COUNT          822 a         1043 c         1169 g          812 t
ORIGIN      
        1 caccctccgc caagaggcta tacgctcgtc attccgcatg gtcaggaaat cgttccggtc
       61 cggaatgcgg ccgtgtggga gggcggcaat gaaatcccgg gatggcgtga tcaacaacac
      121 attttccaga ctggcgggct gcggtttacg ccagggaagc agcttgtcga accagccggg
      181 gatcagccgg gggctgaagt gcaggtagag cacaatgcca ctgtcgtacg gtagcggggt
      241 gtcgaagtga tagtctgtaa tgcccccgtc aacacagctg ccgcctgagg tgccgggaat
      301 gtcctgaacg gcctgcatga tgacgggaat ggaggctgtc gccatcaggg caggacgcag
      361 gttcgtgctg tccagcatgg cattttcttg catgaacagc gtttccctga atggtagctg
      421 gctgtgctgc ggtgcaaaca gcacacgctc gaagaagtgg ccgaggtggc gtcggcctac
      481 gccgttcatc agggcggcag cgccgagctg ccatttccca ggctccccgc cggcattcag
      541 gaggagggag cggcatcgac tggcaatggt atgcagtcgg cggtgcggat tggtgacaat
      601 tgccgagtgt tctttgccca gcagcgcatc aagaatcctg ccacattccc ggctgatctc
      661 gctcatgtcc ggcttggtgc tgtagtgctg ctcaaagtaa gcggcctcga accgttcaat
      721 tgccgtcagc ggatcgggct gggcataact ggcaaggcgc cagctcccaa tggatgtgcc
      781 cagcaggtcc atgtcgacgt ccgggtcagc ggccaatctg ggggcaagga gtcggtcaag
      841 ctgattcagg accaaccatt tggggcctcc tgaggcgccc agcatggcgc gaatgtcggc
      901 cagccgcagt ccgttttcct gcaggtggcg tagtgcccgg ggacctgctg cgatatgaag
      961 tgatgcggtc atttgccaag ggcttcctgc catacctttt cgtcaaaccc gcacaacagc
     1021 cgcgggccga aggccagtac cgggcgcttg atcagcgagg gttgctgctg cattaaaagt
     1081 aacgcaaggt cacgattgat attcgcctgg gtacttgcat ccagcttgcg ccaggttgtg
     1141 ccgcggcgat tcagcagtgc ctcccagccc agtgaacttt cccagcgggc aagtgtggcg
     1201 gcgtcgatac cggacgtctt gtagtcgtga aatgtaaagg caacctcctg actgttcagc
     1261 caggcaaagg ctttcttcat ggtgtcgcag tttttgatgc cgtaaatggt gatcatgccg
     1321 gggctcctgt aaaaaaggca gcttgcgctg cctttttcgt atgggattta ggtatcagtc
     1381 cacgcgcagt aattcgttga tgccaacctt ggatcgggtt tgggcatcaa ccttcttcac
     1441 aatgatggcg gcatagagag agtacttgcc atcttttgag ggcaggctgc cggggactac
     1501 gaccgatcct gcaggcacgc ggccatagtg gatctcaccg gtttcacggt catagatacg
     1561 ggtgctttga ccgatataga cgcccatcga caggacagag ccttcctcaa cgatcactcc
     1621 ctcaaccacc tcggaacggg caccgatgaa gcagttgtct tcgatgatcg ttggattggc
     1681 ctgcaggggt tccagcacac cgccaatccc gacgccaccc gagaggtgaa cattcttacc
     1741 aatctgcgcg caggagccga cagtggccca agtgtcaacc attgagccct catcgacgta
     1801 agcaccaata tttacgtagg agggcatcaa gaccacattg cgggcaatgt aagatccctt
     1861 gcgtgcaaca gcgggaggca cgacacgcac gccggcggca gcgaattgct caggcgtcca
     1921 gtctgcaaac ttggtgggga ccttgtcaaa aaactgcatg ccaccaccgc cagcaatggg
     1981 ggcattgtca ttcaggcgga acgagagcag cacggccttc ttcagccact ggttgacaat
     2041 ccagccacta tcggtcttct cggcgacgcg caacttgccg gcatccagtg cggccaatgc
     2101 ttcgttcacg gcagtacgca catccgatgg tgtgttggca ggcgaaaatt cggcgcgacg
     2161 atcgaaggca ttctcaatca gttgttgcaa gctcatgcgt gggtctccat ctctaatcta
     2221 aaggcaatga aatctgtcac gggcgcagtt agtcgcctgg gcaggcactt tcaaactcaa
     2281 agggatttca tgaagtcgcg aaggcgtctg gccgcctccg cacactcatc gggtgatgca
     2341 accagcgcca ggcggacatg gctggcgcca ggattgcagt cattgatatc gcgtcccagg
     2401 aatcgaccgg ggagtacggt aacatttttt tcctgataca gccgctgggc aaagacatca
     2461 tcgtcaatcg gcgtacgcat ccagaaataa aagccggcat ccggcatcgt cagcggatac
     2521 acaggcgcca tgatggctct gaactcttcg aacttctgac ggtatagggt acggttctgg
     2581 ataacgtgct cttcatcgct ccaggcggcg acagacgcct gctggttttg caccggcatt
     2641 gccgcgccgt ggtaggtgcg gtacagcaga tagggtttca gcagctttgc atcgcctgcc
     2701 acaaaacccg aacggagtcc gggtagattg gaacgcttgg acaatgaatg gaagacgatg
     2761 cagcgtgcat agtcatgccg accgatttcg gcgcacacct gaagcagtcc tacgggagga
     2821 tgcttctcgt cgaagtagat ttccgagtag cactcgtctg acacgatgat gaagttgtgt
     2881 ttgtcggcca gtgcaatcag cttggacagt gtttcgcggc tggtgacagc gccggtcgga
     2941 ttcccgggtg agcagacaaa cagcatctgt gtgtttgccc agatagagtc cggaatggcg
     3001 tcgtaatcgg gaatgaaccc ggtgtcggct gagcagggca gataataggg ctctgctccg
     3061 gccagatagg cggctccttc ataaatctgg tagaacggcg ccggcatcag gaccaaggct
     3121 ttgtgggagc ggtcgatggc ggcctgtgta aacgaaaaca gggcttcgcg ggttcccata
     3181 acgggaagga tctcgctttc ggcatcgact ttttgcaaat gaaaccgctt ttgcaaccag
     3241 gaggcgatgg cttcgcgcag ggccggaaga cccttcgtcg tggggtacac ggacagcccg
     3301 gacagattgt ccgtcaggac ctgtttcaca aattccggtg acgcatgctt gggttcgccg
     3361 atggataacg atatcggtga caggtcggcg gcaggcgaca gccccttgaa aagctgtgca
     3421 agtcgctcga aaggataggc ttgcaggcgg gcaagttctg gattcatggc ggcgctcctt
     3481 acccttcagt ttagctggtc cagctgctcg cgcaggcggc tagccagttg ttcgcagaca
     3541 gcaggatcag aaatcggatg ttgttgtttg tcagtgatga agaaaacgtc ttcagcgcgt
     3601 tcacccaggg tcgcaatgcg cgcattctgc aggacgacgc ccgactccat gaagattcgg
     3661 ccaatacgag ccaacaatcc ggggcggtca agcgtcatga cctcaaggat cgtgcgctgg
     3721 ttgacgatgt cattgctgat ggtgacggtc gtcttgacgt caaaatgctt aagctggcgt
     3781 ggcatgcggc gctggacaat ttccgggaaa cgctccggat tcgacagcgt ctgcaccagc
     3841 gtgtcg
//
LOCUS       DASUEG010000840         9684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1446767, whole genome shotgun sequence.
ACCESSION   DASUEG010000840 DASUEG010000000
VERSION     DASUEG010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446767"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..627
                     /locus_tag="VFX11_15825"
     CDS             13..627
                     /locus_tag="VFX11_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4940148.1"
                     /translation="MYTANNPLLQGNPDLKPETVDTYEVIWLHQWQRAQVSANYFYNR
                     FDDAIRTVVVNSGRTYQNSSESETSQGLTFELTGAVFEHFTTRVAATRLLDTPDAFFR
                     EARQWLVIAGTYTRPQGYGSLTLQYQGERENLHSSADERETLDDYVVVSARLGWTPRN
                     DLETYLDLRNLTDTESSVPSNSGDIDGGVPNRGREVRVGMVVRY"
     gene            620..733
                     /locus_tag="VFX11_15830"
                     /pseudo
     CDS             620..733
                     /locus_tag="VFX11_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
     gene            complement(782..1819)
                     /locus_tag="VFX11_15835"
     CDS             complement(782..1819)
                     /locus_tag="VFX11_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX4940149.1"
                     /translation="MSRHYQPPYSITPTIIRLISEISEQLGRLSVLEDEKNLRLRRIN
                     RVRTIQGSLAIEGNTLSEEQITAILEGKRVIAPPREIQEARNAIKAYEQFEHWQPAIE
                     KDFFKAHQVLMAGLIDDAGRYRRGNVGVMNGEMVVHMAPPANRLKKLMGDLFRWLNST
                     DQHPLITSSVFHYEFEFIHPFADGNGRMGRLWQTLILSQWNPTLAQLPVESMVHAHQS
                     EYYQAINLSTRQTDSAPFIEFMLGVILETIENNVTGTGAHRSPQVAPQVAPQVKLLIC
                     IMKVQPDAMSREDLQKALGLTDRKSFRERYLNPALGAGLIEMTIPDKPNSRQQQYRLT
                     AQGRELAEIVR"
     gene            complement(1839..2513)
                     /locus_tag="VFX11_15840"
     CDS             complement(1839..2513)
                     /locus_tag="VFX11_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4940150.1"
                     /translation="MSNSDSQPTRSLNITEALREQLLQGEFPPHARLQEIALAERMGV
                     SRTPIREALSVLARDGLLEYAPNRGYTVRHFSLDDILSAFRVRAVLEGLGCRLLAERG
                     LPADTDTQLQHTIAWGDTLLQNTQLQQGQFDQWREMNKAFHLAILLATQNDLLIRFAK
                     ISRNIPIVFNGSFRWYSTRDFRRSQDHHEVIYSAMKNRQPERADFMMQEHIMQAAEIL
                     RRNYPV"
     gene            complement(2514..3674)
                     /locus_tag="VFX11_15845"
     CDS             complement(2514..3674)
                     /locus_tag="VFX11_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940151.1"
                     /translation="MSTSTDTSLSAADRELFRDNFRKFLANEVEPHYAQWEKNEIFPR
                     ELWNKMGENGYLCVDMPEAYGGYGADFGMSAIVVEELGYAGYGALMSSVSVHSDIVAP
                     YILHLGTEEQKLKWLPKMVTGEAVGAIGMTEPGAGSDLQAMRTSAVKQGGNYVINGQK
                     TFITNGQHCDVIVLATKTDPNAGSKGITLFTVDCTTPGFQRGRNLEKMGLHSGDTSEL
                     FFDNVTVPESEILGGLGKGFANLMNELPRERLILAVGAIGACDGMLQWTIDYTTERKV
                     FNGTVAQLQNTRFKLAEMKTQIEVNRAYVEKCTEKYVRGALSTVEASMTKLSTTELQG
                     KVADECLQLFGGYGYMMEYKIARAFIDARIQRIYGGTSEIMKEIIARSLVGR"
     gene            complement(3737..4675)
                     /locus_tag="VFX11_15850"
     CDS             complement(3737..4675)
                     /locus_tag="VFX11_15850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940152.1"
                     /translation="MMSNGIPVQQRVLVIDDEKPNLKILSDILRNEVDVILAKGGEQG
                     FDKAVRFRPDLILLDVVMPGLDGFQIIDQLRRDERTSTIPVIFISALSDVGHEEKGLM
                     LGACDYIHKPFHAAIVQARVRLHLQLSRQRLLLEQLAHIDPLTAIANRRRYEEVLQTE
                     WHAAARYRTGIALVMVDIDNFKHYNDFYGHAAGDKVLQTVARVISAQLRRPRDFVARY
                     GGEEFVVLLPESNCQGSIDIMQSCCHAVESLRIDNEPVREHPFVTISVGGVSCLPGVL
                     HTPDSFLKIADDMLYRAKHEGKNRVVWHDAEDGGGA"
     gene            complement(4688..>9684)
                     /locus_tag="VFX11_15855"
     CDS             complement(4688..>9684)
                     /locus_tag="VFX11_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940153.1"
                     /translation="EDGIIFYQREQNRWCWDDERIRSLSLADNVVELLSRKLRLYPAG
                     TCRLLMLASLLGTRFRLEALALVAGLDLPVCLEGLQPVLAEGLLSALDDHHKQPDKLH
                     KLASAQFKFPHDRIQQAAYEIACAEYPNIAALKLEIGRALLALERRKQSREPLFDIVE
                     HINCGRALVTDRDERLDYARLNLALGLQAKKASAYQPAWQCLLAARDFLGEDLALQET
                     ALAIDIHRELAECAYLDGEFPLADAQYPLLAELAVQPIDRIRYLSVQANQYQLQGRFH
                     DALRVINAGIALAGIRFPATAAEMTDQITQEYSQLVEQFSRLTPQELHARPDMTQPLL
                     VAAMELLRVQWYASYLVGDVQLNSLIAVTIARLSLEKGFCDVSAFGFVTSALVASGLQ
                     SDPGLANVLGEFGIELADSRDNRFIRGTAYLLYTTFTRHWHHPVQSSEKYFRIAWECS
                     VEASDYVSAGYVINVRSSDRLIAGRPLAELEALYRQELEYLRKVKQKDMEDATVAGGL
                     QPVLALMGKTSGPFSFDDSQFSEQRFLSTYRETGLHQAYFYQARIRHAFILQTPDLAE
                     MADKYRLVEQFVPGQCKVSEANFYGALIHLQLANGNDEHLATAQEIRAKFMHWQIWCK
                     ANFLHKRLLLDAEIARVTGHDNEAHLLYEQAIDEAEIAGFIQCAAVASERYARFLQDR
                     GMGKSAGFYINRAWQGYAQWGANAKLDQLARQWQQVRFERRGSLTTGRDGQSLDVQAL
                     FKAATLISREVKRPDLTDTLLQLLKEYSGATYGALIHVEGARLSLLAHTNGRNGRTVL
                     YAPDEADLMQEEVQQAVPTGIVSYVHKTRTTRLMNNPSEWDDWGASVYFNQRRPLSII
                     CHPIAGQSGIIAILYLENDLTTHAFNSAKMQAVSLIAQQAAIAIENAALYEQMESRIQ
                     QRTRELAEAKQRAEVATEAKSSFLAKMSHEIRTPMNAVIGLSRLALKTPLDGEQRDYV
                     SKILESAEQLLGLINDILDFSRIEAGKLTIENVPFGLETLIRQAVNLNALKASVKGLE
                     LVIDIDHSIPRQLRGDPLRLQQILVNLISNAVKFTYQGSVQVKVLCRNQSGRKLMLQC
                     SVIDTGIGMSEEQQSRLFESFTQADDSVTRRYGGSGLGLAICKQLCELMGGRIWLESE
                     PGKGTRFHFTIVLDKLEDPADAESSQRSALASLKALVVDDSPLVRTVLLDMLDNLGIK
                     ADQADNGSDALAMVQRADSRGLAYDFILMDWRMPGMDGLEAARRIRQNNPTATTPIFM
                     MSAHDREMLKSGSGDMGALPFIEKPISQSTLLDAINLVLNPGDRPAGLPTDISLNAAP
                     DLSDCRLLLVEDNGINRQVALGFLKDTGASVDVAENGRIALEKLQQERYDLVLMDVQM
                     PEMDGLTATREIRLRLGLRQLPVVAMTAHAMDADMEKSRKAGMDGHLCKPLDPGLLYR
                     TLLQYLQPGGSRVGPPLPVETSLPAGATSLLHRLALVKGLDARQAVRRLGDKAALYEA
                     LIQDFHHEQQAASNRLRQLFQDGEREVLSRMVHSLRSSAAYIGAYNLSRRCAVLESAL
                     DQGGGRESQLEALIAELDSLLLQLQPLLTPSATMVPAALPDGDLAHILIRLLPLLRQS
                     DFAAETLIPTLQQRGAGTGHAEAVARIAQNIRDIEYENAAAIAAEVLAELGGQG"
BASE COUNT         1736 a         2893 c         3040 g         2015 t
ORIGIN      
        1 caggccgatg agctgtatac cgccaacaat cccctgctgc agggcaatcc agacctgaaa
       61 ccggaaaccg tcgatacgta tgaagtaatc tggctgcatc aatggcagcg tgcacaggtc
      121 agcgccaatt acttctacaa ccgttttgac gacgcgatcc gcaccgtcgt cgtgaacagt
      181 ggccgcacct atcagaacag cagcgaaagc gagaccagcc aggggctgac attcgaactg
      241 accggtgccg ttttcgagca tttcaccacg cgtgttgcag ccacccgcct gctcgacacg
      301 cccgatgctt ttttccggga agcacggcag tggctggtga ttgcgggcac ctacacccgg
      361 ccccagggct acggtagcct gaccctgcaa taccagggcg aacgcgaaaa cctgcacagc
      421 agtgctgacg aacgggaaac gctggatgac tatgtggtgg tgagcgcccg gctgggttgg
      481 acaccgcgca atgatctgga gacctatctg gatttgcgca atctgaccga cacggaatcc
      541 tccgtgccgt ccaacagtgg cgatatcgac ggtggcgtgc cgaaccgggg ccgtgaagtc
      601 cgggtaggga tggtcgtgcg ctattgataa gcagattgcc gcaactgcgc tgacccacgg
      661 gctggcgcgg gtgacgcgca acgagaccga ctttgccaga accggggtta gcgtgctgaa
      721 cccgtttgag taaccttcga gcgaaatgcg tttcaggcaa cattgcagcc gaaagcttca
      781 gctagcgaac gatttcggcc aattcccggc cctgcgccgt caatcggtat tgctgctgtc
      841 gactgttggg cttgtcgggg atggtcattt caatgaggcc ggcgcccaac gccggattca
      901 ggtaacgttc gcgaaaggat ttgcggtccg taagccccag ggccttttgc aggtcttccc
      961 ggctcatggc atctggttga actttcatga tacaaatcag taatttgact tggggggcga
     1021 cttggggggc gacttggggg ctccgatgcg cccctgttcc ggtcacatta ttttcaatcg
     1081 tctccagaat cacacccagc ataaactcaa taaacggtgc ggagtcggtc tgtctggtgc
     1141 tgaggttaat ggcctggtag tactcgcttt gatgcgcatg caccatgctt tcaaccggaa
     1201 gctgagccag tgttggattc cattggctca atatcagcgt ttgccacaaa cgccccatgc
     1261 gaccgttgcc atcggcaaag gggtggataa attcgaattc gtagtggaag accgagctgg
     1321 tgatcaatgg atgctgatcc gtggaattca gccaacgaaa caaatcgccc atgagctttt
     1381 tgaggcgatt ggcgggcggc gccatatgca ccaccatttc gccattcatt acgccaacat
     1441 ttcctctgcg atagcgtccg gcgtcgtcga tcaatcccgc catcagtacc tgatgggctt
     1501 taaaaaaatc cttttcgatg gcgggttgcc agtgctcaaa ttgctcgtag gctttgatgg
     1561 cgttgcgggc ttcctggatt tctctgggtg gcgcaatcac tcgtttgcct tccagaatgg
     1621 cagtaatttg ctcttcgctt aatgtgttgc cttcaatcgc aagtgaacct tgaatggtac
     1681 gtacgcgatt gatacggcgc aggcgcaggt ttttttcatc ctccagcaca gacaaacgcc
     1741 ccagctgttc gctgatctca gagatcagcc gaataatggt gggagttatg ctgtagggtg
     1801 gctggtagtg cctgctcatt gttccgcccc gtaacctctc acaccggata attccgccgc
     1861 aaaatttccg ctgcctgcat gatgtgttcc tgcatcatga aatccgcccg ttccggctgg
     1921 cggtttttca tggcgctgta aatcacttca tgatgatcct gcgaacgccg gaaatcccgg
     1981 gtgctgtacc agcggaaaga gccgttgaac acgatgggaa tgttgcggga aatcttggcg
     2041 aagcggatca gcagatcatt ttgggtggcc agcaggatcg ctaggtgaaa ggccttgttc
     2101 atttcgcgcc actggtcgaa ctggccctgt tgcagctggg tgttctgcag cagggtgtcg
     2161 ccccaggcaa tggtatgttg cagctgggta tcggtgtcgg cgggcaggcc ccgttccgcc
     2221 agcagacgac aacccaaccc ctccagcacg gcacgcacgc ggaacgcact cagaatatcg
     2281 tccagcgaaa aatggcgcac ggtgtagccc cgattgggcg cgtattccag caggccatcc
     2341 cgtgccagca cactcagcgc ttcgcggatc ggcgtgcgtg acacgcccat gcgctcggcc
     2401 agtgcaattt cctgcaggcg cgcgtgcggg gggaattcgc cctgcagcaa ttgctcgcgc
     2461 agggcttcgg tgatgttgag ggaacgggtg ggctgggagt cgctgttgct caatcagcgg
     2521 cctaccagcg agcgggcaat gatttccttc atgatctcgg aggtgccgcc gtaaatgcgc
     2581 tggatgcgcg catcgatgaa cgcacgggcg atcttgtatt ccatcatgta gccgtagccg
     2641 ccgaacagct gcagacactc gtccgccact ttgccctgca attcggtggt ggacagtttg
     2701 gtcattgacg cttccacggt ggacagcgcg ccacgcacat atttttccgt gcacttttcc
     2761 acgtaggcac ggttcacttc gatctgggtt ttcatttccg ccagcttgaa gcgggtgttc
     2821 tgcagttgcg ccacggtacc gttgaacacc ttgcgctcgg tggtgtagtc gatggtccac
     2881 tgcagcatgc cgtcgcaggc accgatggca cccacagcca ggatcaggcg ttcgcgcggc
     2941 agttcgttca tcaggttggc gaagcccttg cccaggccac cgagaatttc gctttccggc
     3001 acggtgacat tgtcgaaaaa cagttccgag gtgtcaccgg aatgcagacc cattttttcc
     3061 aggttgcggc cgcgctggaa accgggcgtg gtgcaatcga cggtgaacag ggtgataccc
     3121 ttggagccgg cgttgggatc ggtcttggtg gccagcacga tcacgtcgca atgctggccg
     3181 ttggtgatga aggttttctg gccgttgatg acgtagttgc cgccctgttt caccgcgctg
     3241 gtgcgcatgg cctgcaggtc ggagccggca ccgggttcgg tcatgccgat agcgcccacc
     3301 gcttcaccgg tcaccatttt cggcagccac ttcagtttct gctcttccgt tcccaggtgc
     3361 aggatgtagg gcgccacgat atcggaatgc accgacacgc tggacatcag cgcaccataa
     3421 ccggcataac ccagttcctc caccacgatc gcgctcatgc cgaaatcggc gccataaccg
     3481 ccgtaggctt ccggcatgtc cacgcacagg tagccgttct cgcccatctt gttccagagt
     3541 tcgcgcggga agatttcgtt cttttcccac tgggcgtaat ggggttccac ttcattggcg
     3601 aggaatttgc ggaagttgtc gcggaacagt tcgcggtcgg cggcgctgag gctggtgtcg
     3661 gtgctggtgg acatgggggt actccggttg aggcaaaaag cggattgtat acaagtgccc
     3721 ggcccctgcg aaggcgtcag gcgccgccgc cgtcctcggc atcatgccag actacccggt
     3781 ttttgccttc gtgcttggcg cgatacagca tgtcgtcggc gattttcagg aagctgtccg
     3841 gcgtgtgcag gacacccggc aggcaactca cgccacccac gctgatggtg acgaagggat
     3901 gctcgcgcac cggttcgttg tcgatgcgca gtgactccac cgcgtggcag caggactgca
     3961 tgatgtcgat actgccctgg cagttgcttt ccggcagcag caccacgaat tcctcgccac
     4021 catagcgggc gacgaaatcc cgcggccgcc gcagttgcgc cgagatcacc cgtgccaccg
     4081 tctgcagcac cttgtcgcca gcggcgtggc cgtagaagtc gttgtaatgc ttgaaattgt
     4141 cgatatccac cattaccagc gcgatgccgg tgcggtaacg ggcagcggcg tgccattcgg
     4201 tctgcagcac ctcctcgtag cggcgccggt tggcgatggc ggtgagggga tcgatgtggg
     4261 ccagctgttc cagcagcagc cgttgccgcg acagctgcag atgcagccgc acccgcgcct
     4321 gcacgatggc ggcatgaaag ggtttgtgga tgtagtcgca ggcccccagc atcaggccct
     4381 tttcttcgtg gcccacgtcg gacagggcgc tgatgaagat caccggaatg gtgctggtgc
     4441 gttcgtcacg gcgcagttgg tcgatgatct ggaagccgtc cagaccgggc atcaccacat
     4501 ccagcaggat caggtccggg cggaaccgta cggccttgtc gaagccctgc tcgccgccct
     4561 tggcgagaat gacgtccacc tcgttgcgca ggatgtcgct gaggattttc agattgggtt
     4621 tttcatcgtc gatgaccaga acccgctgct gcaccggaat gccgttggac atcatcagca
     4681 gggctcctca gccctgaccc cccagttcgg ccagtacttc ggcagcaatg gcggctgcat
     4741 tttcatattc gatatcccgg atgttctgcg caatgcgcgc aacggcttca gcatgaccag
     4801 tgcccgcgcc ccgttgttgc agcgtcggaa tcagggtttc ggcggcgaaa tccgattgcc
     4861 tgagcagtgg cagtaatcgt atcagtatat gcgctaaatc cccgtccggc agggcggcgg
     4921 gtaccattgt cgcagacggt gtcagcaggg gctgcaactg gagcaacagc gaatccagtt
     4981 cggcgatcag cgcctccagc tgggattcgc ggcccccgcc ctggtccagc gcgctttcca
     5041 gcacagcgca gcggcgtgac aggttatagg cgccgatata cgcagcactg gagcgcaggg
     5101 aatgcaccat gcggctcagc acttcgcgct cgccgtcctg gaacagctgg cgcagacggt
     5161 tgctggcggc ctgctgttcg tggtggaaat cctggatcaa cgcttcgtag agggcggcct
     5221 tgtctcccag ccggcgcacg gcctgacggg catccagccc tttcaccagc gccagccggt
     5281 gcagcaggct ggtggcaccg gcgggcagtg acgtttccac cggaagcggg gggccaacgc
     5341 ggctgccgcc gggttgcaga tactgcagca gggtgcggta cagcaggccg gggtccagcg
     5401 gcttgcacag gtggccatcc atgccggcct tgcggctttt ctccatgtcg gcatccatgg
     5461 cgtgggcggt catggccacc accggcaact ggcgcaggcc cagtcgcagg cggatttcgc
     5521 gggtggcggt gaggccgtcc atttccggca tctgcacgtc catcaacacc aggtcgtagc
     5581 gttcctgctg cagcttttcc agggcgatgc ggccgttctc ggcgacatcg acactggcgc
     5641 cggtgtcctt caggaaaccc agggccacct ggcggttgat gccgttgtcc tccaccagca
     5701 gcaggcggca gtcggacagg tccggcgcgg cgttgaggct gatgtccgtc ggcaggccgg
     5761 cagggcggtc gcccgggttc agcacgagat tgatcgcatc cagcagggtg gactggctga
     5821 tgggcttttc gatgaagggc agagcgccca tgtccccgct gccggatttc agcatttcac
     5881 ggtcgtgggc cgacatcatg aagatcgggg tggtggcggt cggattgttc tggcggatgc
     5941 ggcgggccgc ctccagtccg tccatgccgg gcatgcgcca gtccatcagg atgaagtcgt
     6001 aggccaggcc ccggctgtcg gcgcgctgca ccatggccag ggcgtcgctg ccgttgtcgg
     6061 cctggtcggc cttgatgccc aggttgtcga gcatgtccag cagtacggtg cgcaccagcg
     6121 gcgagtcgtc caccaccagg gctttcaggc tggccagcgc cgaacgctga gacgattcgg
     6181 cgtcggcagg gtcttccagt ttgtccagca cgatagtgaa gtggaagcgg gtgcccttgc
     6241 ccggttcgct ctccagccag atgcggccac ccatcagttc gcacagctgt ttgcagatgg
     6301 ccaggcccag gccgctgccg ccataacggc gggtgacgga atcgtcggcc tgggtgaagg
     6361 attcgaacag gcgactctgc tgttcctcgc tcatgccgat gccggtgtcg atcaccgaac
     6421 attgcagcat gagcttgcgt ccgctctggt tgcggcacag gaccttcacc tgcacgctgc
     6481 cctggtaggt gaacttcacc gcattgctga tcaggtttac caggatctgc tgcagccgca
     6541 acggatcgcc gcgcagctgg cgcgggatgc tgtggtcgat gtcgatcacc agctccagcc
     6601 ctttcaccga cgccttcagt gcattcaggt tcaccgcctg ccggatcagg gtctccagtc
     6661 cgaacggtac gttttcaatg gtcagcttgc ctgcctcgat gcgcgagaaa tccaggatgt
     6721 cgttgatcag gcccagcagc tgttcggcgg attcgaggat cttgctgaca tagtcgcgct
     6781 gctcgccgtc cagcggcgtt ttcagcgcca gccggctgag accgatcacc gcgttcattg
     6841 gcgtgcggat ttcatgactc atcttcgcca ggaaactgga tttggcttca gtggccactt
     6901 ccgcgcgctg tttggcttct gccagttcac gggtgcgctg ctggatgcgg gattccatct
     6961 gctcgtacag cgcggcattc tcgatggcga tggcagcctg ctgggcgatc agcgacaccg
     7021 cctgcatctt cgcgctgttg aaggcgtgag tggtgagatc gttttccagg tacaggatgg
     7081 cgatgatgcc ggactggccg gcaatcgggt gacagatgat ggacaacggc cggcgctggt
     7141 tgaaatacac cgacgcgccc cagtcatccc attcgctggg attgttcatc agccgggtgg
     7201 tgcgggtttt gtgcacatag ctgacgatgc cggtgggcac cgcctgctgc acttcttcct
     7261 gcatcaggtc agcttcgtcc ggcgcgtaca gcacggtgcg gccgttgcgg ccattggtgt
     7321 gggccagcag gctgaggcgc gcaccctcca catgaatcag cgcgccatag gtggcgccgg
     7381 aatattcctt caacagctgc agcagcgtgt cggtgagatc cgggcgcttc acttcccgcg
     7441 agatcagggt ggccgccttg aacagggcct gcacatccag cgactggcca tcgcgtccgg
     7501 tggtcaggct gccgcgtctt tcaaaccgca cctgttgcca ttgccgggcc agctgatcga
     7561 gcttggcgtt ggcgccccac tgggcatagc cctgccaggc ccggttgatg tagaagcctg
     7621 cacttttgcc catgccgcga tcctgcagga agcgggcata gcgttcgctg gccacggcgg
     7681 cgcactggat gaagccggca atttccgctt cgtcgatggc ctgctcgtac agcaggtgcg
     7741 cttcgttgtc gtgcccggtc acgcgggcga tttccgcatc cagcagcagt cgcttgtgca
     7801 ggaagttggc cttgcaccag atctgccaat gcatgaactt ggcgcggatc tcctgtgcgg
     7861 tggcaaggtg ttcatcattg ccattggcca gctgcaggtg aatcagcgcg ccgtagaaat
     7921 tggcttccga caccttgcac tggcccggca cgaactgctc caccagccgg tatttgtctg
     7981 ccatctccgc cagatccggc gtctgcagga tgaaagcgtg acggatgcgc gcctggtaga
     8041 aataggcttg gtgcaggccg gtttcgcggt aggtggacag gaagcgctgt tcggaaaact
     8101 ggctgtcgtc gaagctgaac gggccgctgg ttttgcccat cagggccagc accggctgca
     8161 ggccgccggc gacggtggcg tcctccatgt ccttctgctt taccttgcgc aggtattcca
     8221 gttcctggcg gtacagtgct tccagttccg ccaggggccg cccggcgatc aggcggtcgg
     8281 aggagcgcac gttgatgaca tagccggcgc tgacgtaatc gctggcttcc actgaacatt
     8341 cccaggcgat gcggaagtat ttttccgacg attgcaccgg gtggtgccag tgacgggtga
     8401 acgtggtgta gagcaggtag gcggttccgc ggatgaaacg gttgtcgcgg ctgtcggcca
     8461 gttcgatgcc aaactcgccc agcacgttgg ccaggcccgg atcggattgc aggccgctgg
     8521 ccaccagggc gctggtgacg aagccgaacg cggacacatc acagaaacct ttttccagcg
     8581 agagccgggc gatggtgacg gcgatcaggc tgttgagctg cacgtcgccc accagatacg
     8641 aggcgtacca ctgcacccgc agcagttcca tcgcggccac cagcaacggt tgggtcatgt
     8701 cggggcgggc gtgcagttcc tgcggcgtca ggcgcgaaaa ctgttccacc agctggctgt
     8761 attcctgggt gatctggtcg gtcatttccg ctgccgtggc ggggaagcgg atgccagcca
     8821 gggcaatgcc tgcattgatc acccgcaagg cgtcgtggaa gcggccttgc agctggtact
     8881 ggttggcctg caccgacagg tagcggattc tgtcgatggg ctggacggcc agttccgcca
     8941 gcaacgggta ctgcgcatcc gccagcggaa attcgccgtc cagataagcg cactccgcca
     9001 gttcgcggtg aatgtcgatg gccagtgccg tttcctgcaa cgccagatcc tcgcccagaa
     9061 aatcccgtgc cgccagcagg cattgccagg ccggctggta cgccgacgcc tttttcgcct
     9121 gcagacccag ggccaggttg aggcgggcgt agtcgagacg ctcgtcgcga tccgtcacca
     9181 gggcgcggcc gcagttgatg tgttcaacga tgtcgaacag cggctcgcgg gactgcttgc
     9241 gccgttccag tgccagcagg gcacggccga tctccagctt cagtgcggcg atgttgggat
     9301 attcggcgca agcgatttca taagcggcct gctggatgcg gtcatgggga aacttgaact
     9361 gggcgctggc cagcttgtgc agtttgtcgg gctgcttgtg atggtcatcg agcgcgctga
     9421 gcagcccttc cgccagcacc ggttgcaatc cctccaggca caccggcagg tccaggcccg
     9481 ccaccagcgc cagcgcttcc aggcggaaac gggtacccag cagcgaagcc agcatcagca
     9541 gccggcaggt gcccgccgga tacagccgca gcttgcggct tagcagttcc accacattgt
     9601 ccgccaggga caggctgcgg atgcgctcgt catcccagca ccagcgattc tgttcgcgct
     9661 ggtaaaaaat gatgccgtcc tcgt
//
LOCUS       DASUEG010000841         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1093679, whole genome shotgun sequence.
ACCESSION   DASUEG010000841 DASUEG010000000
VERSION     DASUEG010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093679"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2575)
                     /locus_tag="VFX11_15860"
     CDS             complement(<1..>2575)
                     /locus_tag="VFX11_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940154.1"
                     /translation="EFPYVIVTTDYPGASPEVVENDVTRRIEEAINTVPGVKRLISTS
                     FEGRSRIAVEFDLGVPIGVAVQDVRDKVAVTKTRFRDEVSEPLIERYRPDESPVFSMA
                     FRSDSLSPRELSTVVQQQVVRRLQNVAGVGRVDVVGAVPREIRIELIPERMEAVGVGV
                     DQVMAALLRDNAELPAGTVAMGAQERIVQVAGRIEDPQDFGSLVVAVRGQVAITLDQV
                     ARLVDGEHEAQSLALLNGRRAISVDIVKMSGANTIALVDALQAQWPVLQDMLPPGVEM
                     IQVADTSRSVRASLADVQKTLIEGAALAMVIVFLFLASWRSTVITGLTLPISLLGTVF
                     CLHLFGLSLNIMTLMAMSLCIGLLVDDAIVVRENIVRHAAMGKSHQQAALDGTREIGL
                     AVVATTLTIVAVFLPVAFMGGIIGRFFYQFGIAVSCAVLLSMLVSFTLDPMLSSRWND
                     PDAMGRRGNARIDRLLDYFDRVLTRLSERYDQAIVWALSHRSQTLGIAVLSLLLAVIV
                     SKGVGREFVPEPDLSEIGIKFSTPAGSTLQYSGQKARQVTDIIRGYPGVKDVYATINS
                     GMETGKQRVSLRVMLRSRDERTLSQAELVSVMRERLQEVAGIEVTSVAAARESIGSLK
                     PVQISLQGSDLKELQRLASELEQKMADVPGLVDLESSLREVRPTLALQVDRERAADLG
                     LSVGQIGAALRPLLAGEIVTTWQAPDGESHDVRVQLPAGLRESADDLASLPIVSNSIN
                     PASGFPEMVVLGQVSRMADARGTAQINRRNLYREVLFSANVSGRPAGDVGADIEAVAA
                     TIKMPAGYRVVTQGANNDMKESMGHAVTALLLGALFVYMLLGTQFNSFLHPLTIMTSL
                     PL"
BASE COUNT          599 a          837 c          658 g          481 t
ORIGIN      
        1 aaagaggaag ggaggtcatg atggtcagcg gatgcaggaa gctgttgaac tgggttccca
       61 acaacatgta gacaaacaat gcgcccaaca acagtgccgt tacagcatgc cccatcgact
      121 ctttcatgtc gttgttggca ccctgcgtca ccacacgata gcccgccggc atcttgatcg
      181 ttgccgccac cgcctcaatg tcggccccca catcacctgc cggtcgcccg gaaacattgg
      241 cagagaacag cacctcccga taaaggttac gacggtttat ctgggcggta ccacgagcgt
      301 ccgccatgcg ggaaacctgt cccagcacga ccatttcagg aaaaccggac gccgggttga
      361 tgctgttgct gacgatcggc aaggacgcaa ggtcatcagc gctttcgcgc aatccggccg
      421 ggagctgcac acggacatca tggctctcgc cgtcgggcgc ctgccacgtg gtcacgatct
      481 cgccggccag caggggtcga agtgccgcac cgatctgccc gacagaaagc cccagatctg
      541 ccgcacgctc acgatccacc tgcaacgcca gcgtggggcg aacctccctg agcgagcttt
      601 caagatcaac aagtcccgga acgtctgcca tcttttgctc cagctcggac gccagccgct
      661 gtagctcctt caggtcacta ccttgcaagg aaatctggac gggcttcagg ctgccgatcg
      721 attcacgtgc cgcagcgacg gatgtcacct caatccccgc cacctcctgt aatcgctcgc
      781 gcatgacact gaccagctct gcttgcgata acgtccgctc gtctcgcgac ctgagcatga
      841 ctcgcagtga tacccgctgc ttgcctgtct ccatgccgga attgatcgtg gcatagacat
      901 cctttacacc gggatagccg cggatgatat cagtgacctg ccgggccttc tgtccggaat
      961 actgcagggt agaccctgca ggcgtggaga acttgatgcc gatctcgctc aggtcaggct
     1021 ccggtacaaa ctcccgccct accccctttg acacgatgac cgccaacagc aatgacaaca
     1081 ccgcaatacc caatgtctgg ctgcgatgtg acaaggccca gacaatcgcc tgatcgtatc
     1141 tttccgaaag acgagtcaat accctgtcga aataatccag caaccggtca atcctggcat
     1201 tcccccttcg gcccattgca tcgggatcat tccagcggga ggaaagcatg ggatcaagag
     1261 tgaaactcac cagcatcgac agcaatacgg cacacgacac ggcaatacca aactggtaga
     1321 aaaatcgtcc gatgattccg cccatgaaag ccaccggcag gaaaacagcc acaatcgtca
     1381 gtgtggtggc gacaaccgcc agaccgattt cccgcgtgcc atccagtgct gcctgttggt
     1441 gcgactttcc catcgccgca tgacgaacga tgttctcgcg caccacaatt gcatcgtcca
     1501 ccagcagacc gatacagaga gacattgcca tcaatgtcat gatattcaat gacagtccga
     1561 acagatgaag acagaaaact gttcccagca gggatattgg cagtgtgagt ccggtgatca
     1621 cagtggatcg ccacgacgcc agaaacagga agacgatcac catggcaagt gctgcacctt
     1681 cgatcagtgt cttctgcaca tcggcaagcg acgcccgaac agagcgtgac gtatcagcca
     1741 cctggatcat ctctacaccc ggtggcagca tgtcctgaag aacgggccat tgcgcctgca
     1801 atgcatcaac cagcgctatc gtattggccc ctgacatctt gacgatatca acactgatgg
     1861 cacggcggcc attaagcaat gcaagggact gcgcttcgtg ctcgccatcc accagtcgtg
     1921 ccacctgatc cagcgtaatg gccacctggc cacgaacagc gacaactagc gacccgaagt
     1981 cctgcgggtc ttcaatgcgg ccggcgacct gtacgatgcg ctcctgcgcc cccatggcca
     2041 cagtgcctgc aggaagctct gcattgtccc ggagcagcgc tgccatcacc tggtcaaccc
     2101 caacgcccac tgcttccatc cgctccggta tcaattcgat gcgaatctcg cgaggaaccg
     2161 cccccacgac atccactcga ccgacacccg ccacattctg aaggcgccgt accacctgtt
     2221 gctggacaac cgtactcagc tcacgcgggg acaagctgtc cgagcggaat gccatcgaga
     2281 aaaccggaga ctcgtccggg cggtaacgct cgatcagagg ctcactcacc tcgtcacgga
     2341 agcgcgtctt tgtcaccgca accttgtcac gaacatcctg caccgccact ccgatcggga
     2401 cacccagatc aaattcgact gcaatgcgtg agcgcccttc aaaggaggtc gatatcagcc
     2461 gcttcactcc cggaacggta ttgatggctt cctcgatccg tcgcgtaacg tcattttcca
     2521 ccacctctgg tgaggcgccg ggatagtcgg tggtaacaat gacgtagggg aattc
//
LOCUS       DASUEG010000842         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_713134, whole genome shotgun sequence.
ACCESSION   DASUEG010000842 DASUEG010000000
VERSION     DASUEG010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713134"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="tssF"
                     /locus_tag="VFX11_15865"
     CDS             complement(<1..1771)
                     /gene="tssF"
                     /locus_tag="VFX11_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX4940155.1"
                     /translation="MSDDLLKYYEKELAFVQKAMAEFAQRHPKMGNRLRIASDKAEDP
                     FVDRLLDGVALLNARIQDKLNDDFPELTDALLGTLYPHYQRPIPSMSIVQFVPAAALD
                     QCTQVPAQTLLDTPTFRGESCRFSTCYPADIQPLKVESAALLARPFVAPGADTVGGAD
                     AVLRICLRTLSNELSISTLKPKKVRFYLRGQPKHVYPLYEHLLNESVRLVLTSSEIDN
                     KPLFCEADCIQPVGFDVSEGLLPYPANAFVGYRLLTEYFVFPQKFLFVDIDVSRLPDN
                     ARNELNLYIYLKNSDSELEHYINASTFALGCTPVVNLFPQVADPVRLDHSRHEYRIVP
                     DSRRTSALEIYSVDAVKATDAAGHQFEYTPFYSIQHRHTEKNHGTFWFERRDSVIEGE
                     NKSEQASEIDISLVDLNFDPWLPHDDVLDIQLTCCNRNLPGKLPSGTNQPELRLVEGD
                     APVAAIRCITPPTHTIRPPLRNRAHWRLLSHLNLNHLSLSGPSGTEALKEILRLYDFR
                     DSDATRKMIESICQLHTSAITAPITFDQGTAMCRGTQVEIEFDPMLLSGGSAYLFASV
                     LERFLALYCSINSFTRLVARLQKT"
     gene            complement(1768..2262)
                     /gene="tssE"
                     /locus_tag="VFX11_15870"
     CDS             complement(1768..2262)
                     /gene="tssE"
                     /locus_tag="VFX11_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEX4940156.1"
                     /translation="MAQIDADKKLIAPLLDRLLDDNPSAPHDFTQGQHLLLNQLRESV
                     RRDLEKLFNSRQCCLTPPLEMEHLQGALPNYGLPDAATLNLHSQKARLDFCRTVEEAV
                     RRFEPRIRSVKVFTQSAIDPEDPSIRFRVEAMLYAVTTEELIIFDSALNPVTRSVTVQ
                     ESRK"
     gene            complement(2268..>2821)
                     /locus_tag="VFX11_15875"
     CDS             complement(2268..>2821)
                     /locus_tag="VFX11_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX4940157.1"
                     /translation="FDALPIDAGNAHHILTDIVLTDTLERQLNDLGLMALCQCYGVPL
                     AAFHSNPSLHQAKHFDSKSATANARISAMLQQVLCASRFAHYLKVMIRDKVGSYSTEK
                     ECEDFLLKWLNQYTTGRDDLSWDMRARYPLRDVRLTVRETPGSPGQYSCIIYLKPHYI
                     AENLVTELKLTTELTQSGISTRG"
BASE COUNT          531 a          794 c          893 g          603 t
ORIGIN      
        1 ctgttttttg cagccgggcc accagccgcg taaatgaatt gatcgaacag tacagcgcca
       61 gaaaccgctc cagcacgctg gcgaacaggt atgccgagcc accgctcagc agcatggggt
      121 cgaattcaat ttccacctga gtcccacggc acatggcggt gccttggtca aaggtgattg
      181 gcgcggtgat ggcgctggtg tgcagctggc agatggattc gatcatcttg cgtgtggcat
      241 cggaatcgcg aaaatcatac agacgcaaaa tttccttcag cgcctcggtg ccgctggggc
      301 cactcagcga caaatgattc agattgagat gcgacagcaa ccgccagtgg gcgcgattac
      361 gcaggggcgg ccggatggtg tgggtgggcg gtgtgatgca gcggatcgca gccactggcg
      421 catccccttc caccagcctc aactccggct ggttggtgcc ggacggcagt tttcccggca
      481 gattgcggtt gcagcaggtc agctgtatgt ccagcacgtc gtcgtggggc agccagggat
      541 cgaaattcag atccaccaga ctgatgtcga tttccgacgc ctgctcactt ttgttttcac
      601 cttcgatgac gctgtcgcgc cgttcgaacc agaaggtgcc gtgatttttt tccgtgtgcc
      661 ggtgctggat gctgtaaaac ggcgtgtact cgaactggtg accggcggca tcggtggcct
      721 tcactgcatc cacagaatat atttccaggg cactggtgcg acggctatcg ggcacgatgc
      781 ggtattcgtg gcggctgtga tccagtcgca ccggatcggc cacctgcggg aacaggttca
      841 ccaccggcgt gcaacccagt gcgaatgtgc tggcgttgat ataatgctcc agctcactgt
      901 cggaattttt caggtagatg tacaggttga gttcgttgcg ggcattgtcc ggcagccgcg
      961 acacgtcgat atccacgaac aggaattttt gcgggaagac gaaatattcc gtcaacaggc
     1021 gatagccgac aaaggcgttg gcggggtagg gcagcaggcc ctcgctgacg tcgaatccca
     1081 ccggctggat gcagtcggcc tcgcagaaca ggggtttgtt gtcgatttca ctgctggtca
     1141 gcaccagacg cacggattcg ttcagcaggt gctcgtacag cgggtacacg tgcttgggct
     1201 ggccgcgcag ataaaaacgc acctttttcg gtttcagcgt gctgatcgac agctcgttgc
     1261 tgagggtgcg caggcagatc cgcaacacgg catcggcacc gcccaccgta tcggctcccg
     1321 gcgccacaaa cggacgcgcc agcagcgcgg cagattccac tttcagcggc tggatgtccg
     1381 cggggtagca ggtggaaaag cggcagctct cgccacgaaa ggtcggagtg tccagcagtg
     1441 tttgtgccgg cacctgtgtg cactggtcca gcgcggcggc cggcacgaac tgcacaatgc
     1501 tcatggacgg aatcggccgc tggtagtgcg ggtacagtgt gcccagcagc gcgtcggtga
     1561 gttccggaaa atcgtcgttc agcttgtcct ggatgcgcgc attcagcagc gccacgccat
     1621 ccagcaggcg atccacgaac ggatcttccg ccttgtcgct ggcaatgcgc aggcggttgc
     1681 ccattttcgg atgacgctgg gcgaactccg ccatcgcctt ctgcacgaag gccagttcct
     1741 tttcataata tttcagcagg tcgtcactca tttgcgcgac tcctgcacgg tgacggaccg
     1801 ggtgacggga ttgagcgcgg aatcaaagat gatcagctcc tcggtggtga cggcgtagag
     1861 catggcttcc acccggaagc ggatgctggg atcttccgga tcgatggccg actgggtgaa
     1921 gactttcacg ctgcggatgc gcggctcgaa gcggcgcacg gcttcctcca ccgtgcggca
     1981 gaaatccagt cgcgccttct gtgaatgcag gttcagggtg gcggcgtccg gcaaaccgta
     2041 gttgggcagc gcgccttgca ggtgttccat ttccagcggc ggtgtcaggc agcactggcg
     2101 gctgttgaac agtttttcca gatcgcggcg cacgctttcc cgcagctggt tcagcagcag
     2161 gtgctggccc tgggtgaaat cgtggggcgc ggagggattg tcgtccagca accggtccag
     2221 cagtggcgca atcagtttct tgtcggcatc gatctgggcc atctgcatta tccccgcgta
     2281 ctgatgccgg actgggtgag ttcagtggtg agtttcaatt ccgtcaccag attttccgcg
     2341 atgtagtgcg gcttcaggta aatgatgcag gaatactgcc cgggtgatcc cggcgtttca
     2401 cgcacggtca gccgcacatc ccgcagcgga tagcgggcgc gcatgtccca gctgaggtcg
     2461 tcgcggccgg tggtgtactg gttcagccat ttcagcagaa aatcctcgca ttccttttcc
     2521 gtgctgtagc tgcccacctt gtcgcggatc atgaccttca gatagtgggc aaaacgtgat
     2581 gcgcacaata cctgctgcag catggcgctg atgcgggcgt tagcagtggc gctcttgctg
     2641 tcgaaatgtt ttgcctggtg cagcgacgga ttgctgtgaa atgccgccag cggcacgccg
     2701 tagcactgac acaacgccat caatcccaga tcgttcagtt gccgttccag tgtgtcggtc
     2761 agcacgatgt cggtgagaat gtggtgcgca ttgccagcgt cgatgggcag cgcgtcgaag
     2821 c
//
LOCUS       DASUEG010000843         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_697777, whole genome shotgun sequence.
ACCESSION   DASUEG010000843 DASUEG010000000
VERSION     DASUEG010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697777"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX11_15880"
     CDS             <1..430
                     /locus_tag="VFX11_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX4940158.1"
                     /translation="TLALRDAGLPLPKCNLAYSPMADLTAPHGSRNLNELRDDMFTPE
                     MYHIGIDLYGRTEADRLNPHASPVFGDFTGMPPLLVTVDRSECMHDDSVKVVERARAA
                     GVDVTFIERDGLLHVWPIFYPLLPEARQDVAESVMFIDRH"
     gene            535..>2127
                     /locus_tag="VFX11_15885"
     CDS             535..>2127
                     /locus_tag="VFX11_15885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940159.1"
                     /translation="MNSRTLKLLPLRWLAVCLLTLFCLRPALALESIELGAGVVSLSP
                     APEQLELFQDPADNLRLEDILAGRASFAPVRQQVINLGKSDSSVWVHLRLRDVTPGET
                     VNTWILELAFARLARVDIHLVDNGTLKLSERIGYAVPMSARTLKHNYFAQPLLLEPGR
                     QYDLYLNVMRKGGGIQIPLKLHRATEFSRYVSESNRYYGMYFGIMLAMLIYNSFLMVS
                     VGTRAYLYYILHIIGTFVTFQILQGYAFLYWWPEVPVINEYAIQIAVCLSSMAALLFV
                     RHYVGLHQFAAWMNRVITGMLVLGCSIIAIRLATSQPMIAAAGLFIAGASVLMMTITL
                     ICWRNGSRPAGFFLLAWTVFLLGALLHMLTLFGWFPHNTLSSYGVIIGSALEVLLLSL
                     GLADRINAERRARYLALQEKHAAIIDLKEAEAQLVRRAMHSATTGLPNRAFLRSVLDE
                     ICVDVTSQPSVALLLIGMNNFHEYNKTLGHSNGDAILCLLAERLDQLLQPQPGLVRIE
                     DNGDQRHHLACIEGVTFAALLRG"
BASE COUNT          366 a          652 c          656 g          453 t
ORIGIN      
        1 cacgctggcc ctgcgcgatg cgggcctgcc gttgccgaaa tgcaatctgg cctattcgcc
       61 gatggcggat ctcaccgcgc ctcacggcag ccgcaacctg aacgaactgc gcgacgacat
      121 gttcacgccc gagatgtatc acatcgggat cgacctgtat ggccgcacgg aagcggaccg
      181 gctcaatccc catgcctcgc cggtgttcgg tgatttcacg ggcatgccgc cgctgctggt
      241 gacggtggac cggagcgaat gcatgcacga tgattcggtc aaagtggtgg agcgggcccg
      301 ggccgccggc gtcgatgtga cctttatcga gcgtgatggc ctgctgcatg tgtggccgat
      361 tttctatccg ctgctgccgg aagcgcgtca ggatgtagcc gaatcggtga tgtttatcga
      421 ccgccactga cgccgcagcc ttacttggca cctcgggctc cggaatccct gcctatactg
      481 gattgaaacc tgctattgcc agacggatga cccccgggga ttgccaacac ctgcatgaac
      541 agccggacgt tgaagttgct gccactgcgc tggctggccg tgtgcctgct gacgttgttt
      601 tgcctgcgtc cggccctggc gctggaatcc attgagctcg gcgccggcgt ggtgtcgttg
      661 tcacccgcgc cagaacagct ggaactgttt caggaccctg ccgacaacct gcgcctggag
      721 gatatcctgg ccggccgcgc gagcttcgcg cccgtgcgcc agcaggtcat caatctgggc
      781 aagagcgaca gttccgtgtg ggtacacctg cgcctgcggg atgtcacccc gggagagacg
      841 gtcaatacct ggattctgga actggccttc gcgcggctgg cccgggtgga catccatctg
      901 gtggataacg gcacgctgaa actgagcgag cgcatcggct atgcagtgcc aatgtcggcg
      961 cgcacactga agcacaacta ctttgcccag cctctgctgc tggagccggg ccggcagtat
     1021 gatctctatc tgaacgtcat gcgcaagggc ggtggcatcc agattccgct caagctgcat
     1081 cgggccaccg aattttcccg ctatgtgtcc gaaagcaacc gctactacgg catgtacttc
     1141 ggcatcatgc tggcgatgct gatctacaac agcttcctga tggtgtcagt gggcacccgc
     1201 gcttacctgt attacatcct gcacatcatt ggcactttcg tcacgtttca gatcctgcaa
     1261 ggctacgcct tcctgtactg gtggcctgaa gtgcccgtca tcaatgagta cgccatccag
     1321 attgcagtgt gtctgtcctc gatggcggcg ttgctgttcg tgcgccacta cgtcggattg
     1381 caccagtttg cggcctggat gaaccgcgtg atcaccggca tgctggtgct gggctgctcc
     1441 atcattgcca tccgcctggc aaccagtcag cccatgatcg cggcagccgg gctgttcatt
     1501 gcgggtgcca gtgtgctgat gatgacgatc accctgatct gctggcgtaa cggttcgcgc
     1561 ccggcgggct ttttcctgct ggcttggacg gtattcctgc tgggcgcatt gctgcacatg
     1621 ctgaccctgt ttggctggtt tccccacaac acgctgtcgt cgtatggcgt catcatcggc
     1681 tcggcgctgg aagtgctgct gctgtcgctg gggctggcgg atcgcatcaa tgcggagcgg
     1741 cgcgcccgct atctggcgct gcaggaaaag catgccgcca tcatcgatct gaaggaagcg
     1801 gaggcacagc tggtgcggcg ggcgatgcac agcgccacca ccggactgcc caatcgcgca
     1861 tttttgcgca gtgtgctgga cgaaatctgc gtcgatgtga cttctcaacc cagcgtcgcg
     1921 ctgctgctga ttggcatgaa caacttccac gaatacaaca agacgctggg tcacagtaac
     1981 ggcgacgcca ttctgtgcct gctggcggag cggctcgatc agctgctgca gccacaaccg
     2041 ggtctggtgc ggatcgagga caatggcgac cagcgccatc atctggcctg catcgagggc
     2101 gtgacgtttg ccgccctgct gcggggg
//
LOCUS       DASUEG010000844         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2011072, whole genome shotgun sequence.
ACCESSION   DASUEG010000844 DASUEG010000000
VERSION     DASUEG010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011072"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFX11_15890"
     CDS             <1..681
                     /locus_tag="VFX11_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4940160.1"
                     /translation="RRRMVADISHELRTPIATLRAQLEAIEDGVRPLNASAVQRLHEQ
                     VMRLSNLVHDLYELSLADIGALSYRKQLLDLRELTTTLVNSMQPQFAQAALELTVQDQ
                     CNGSLPVLGDQQRLTQLFANLLQNSLQYTTAPGQVRVHIRAEDQTAVICVEDSAPGVE
                     KSVQSRLFERLFRAESSRNRNTGGAGLGLSLCQTIAEAHNGSIVATDSTLGGLAVTVR
                     IPLTQVAS"
     gene            678..1376
                     /locus_tag="VFX11_15895"
     CDS             678..1376
                     /locus_tag="VFX11_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940161.1"
                     /translation="MNSSTQQPKILIVEDEKALADVLDDYLRHHHFVTRVVEDGALVQ
                     DIVAAFHPDLILLDLMLPNKDGLTICREIRARSAVPIIMTTARVEEIDRLLGLELGAD
                     DYVCKPYSPREVIARVKAVLRRAGAQDASPTSKALVLRPESMSATLLGVALELTAVEF
                     RLLETMANEPRRIFTRDQLMDRIYTDQRIVSDRTIDSHITKLRKKLATANAGADVIRS
                     VYGVGYKYEEPEHG"
     gene            1490..1801
                     /locus_tag="VFX11_15900"
     CDS             1490..1801
                     /locus_tag="VFX11_15900"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX4940162.1"
                     /translation="MKYALALLMIGLFSLSALARMPGPENMLAHLTSALNLTDEQVEQ
                     VETIITDNQTQMQKLMQQMAALRAETDTAIKAVLNEEQLKKFETLQRNRPQRGQPPQD
                     F"
     gene            complement(1873..>2257)
                     /locus_tag="VFX11_15905"
     CDS             complement(1873..>2257)
                     /locus_tag="VFX11_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940163.1"
                     /translation="TPPAQPGYLPDALQTLLLGGIWWFVLIWLPLLQAENAAPELGAV
                     APLLFKVEGVVSALVSLVLIGLRMAAGTTIWRETSLLLGIAVCGVTMVVWVTRPDTLA
                     PDGLTYPAALQGILALACFLVRRPS"
BASE COUNT          500 a          709 c          663 g          385 t
ORIGIN      
        1 cgccggcgca tggtggcgga tatttcccac gaattgcgca cgccgattgc cacgctgcgg
       61 gcgcaactgg aagccatcga ggatggtgtg cggccgctga atgccagtgc ggtccagcgc
      121 ctgcatgagc aggtgatgcg actgtcgaat ctggtgcatg acctgtatga gttgtcgctg
      181 gcggatatcg gcgcactgtc gtaccgcaaa cagttgctgg acctgcgcga actgacaaca
      241 acactggtga actccatgca gccacagttt gcacaggccg cgctggaatt gacggtgcag
      301 gaccaatgca acggctcatt gccggtactg ggcgatcagc agcggctgac ccagctgttc
      361 gccaatctgc tgcagaacag cctgcaatac accacggcgc ccggccaggt gcgcgtgcac
      421 atccgcgcgg aagaccagac tgcggtgatc tgcgtggagg attcggcgcc aggcgtggaa
      481 aagtcggtgc aatcgcgcct gttcgagcgg ctgtttcgcg ccgagtcttc acgcaatcgc
      541 aataccggcg gcgccgggct gggcctgagc ctgtgccaga ccatcgccga agcccacaac
      601 ggcagtatcg tggcaacgga cagcaccctg ggcggactgg ccgttaccgt tcgcatccca
      661 ctgacgcagg tggcctcatg aatagcagca cccaacaacc aaaaatcctg atcgtcgaag
      721 atgaaaaggc actggcggac gtactggatg attacctgcg tcatcaccat ttcgtcaccc
      781 gcgttgttga ggatggcgca ctggtgcagg acatcgtcgc cgcatttcat ccggacctga
      841 tcctgctgga cctgatgctg ccgaacaagg atggcctgac catctgtcgc gaaatccgcg
      901 cccgcagcgc agtgcccatc atcatgacta ccgccagagt ggaggaaatt gatcgcctgc
      961 tgggactgga actgggcgcg gatgattacg tttgcaaacc ctacagcccg cgcgaagtga
     1021 ttgcgcgggt gaaggcagtg ttgcggcgcg ccggtgcgca ggacgcatcg ccgacaagca
     1081 aggcgctggt gctgcggccg gaatccatga gtgccaccct gctgggcgtt gcactggaac
     1141 tgacggcggt ggaattccgt ttgctggaaa ccatggccaa cgaaccgcgc cgcattttca
     1201 cccgcgacca gctgatggat cgcatctaca ccgaccagcg catcgtcagc gaccgcacca
     1261 tcgattcgca catcaccaag ctgcgcaaga aactggcgac ggccaacgct ggcgccgatg
     1321 tcatccgctc cgtgtacggc gtcggctaca agtacgagga accagaacac ggctgacccc
     1381 gaatcgcact ccgtcgcaag cttcacattc gctgcacaat cggtgtgaat ttgctgcgcg
     1441 atgcatccgc atactgcctc ccatgcaaat cactcaaaag gaagcagaca tgaaatacgc
     1501 actggcatta ctgatgatcg gactgttttc gctgtcggca ctggcgcgca tgccggggcc
     1561 ggagaacatg ctggcccacc tgaccagcgc gctcaacctg accgacgagc aggtggaaca
     1621 ggtagagacc atcatcacgg acaaccagac gcagatgcag aagctgatgc agcagatggc
     1681 ggctctgcgg gcggaaaccg ataccgccat caaggccgtg ctcaacgagg agcagctgaa
     1741 gaaattcgag acactgcagc gcaatcgtcc gcagcggggt cagccgccac aggatttctg
     1801 aaccgtaccc acttccccaa tggcatttcc cgatgccggt ttgcgtcacg gctccccaac
     1861 cgtggcgctt ttttatgagg gacggcgtac gagaaaacag gcgagcgcaa gtatgccctg
     1921 caacgctgcc gggtaggtca gaccatccgg cgcaagcgta tccgggcgtg taacccatac
     1981 caccattgtc acaccacaga cggcaatgcc gagcagcaga ctcgtttcgc gccagatcgt
     2041 tgtacccgcc gccatgcgca atccgatcag aaccagactg accagcgcgc tcaccacccc
     2101 ttccaccttg aacagcagcg gtgcgacggc gcccaattcg ggagcagcat tttccgcctg
     2161 cagcaacggc agccagatca gcacaaacca ccagatccca cccagcagca acgtctgcaa
     2221 tgcatcaggc agatagccgg gctgtgccgg gggcgtt
//
LOCUS       DASUEG010000845         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_212918, whole genome shotgun sequence.
ACCESSION   DASUEG010000845 DASUEG010000000
VERSION     DASUEG010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212918"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VFX11_15910"
     CDS             complement(<1..1192)
                     /locus_tag="VFX11_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940164.1"
                     /translation="MNLATWSIHNPIPNILLFFFLTLAGIWGFNQLNIQNFPDMDYPA
                     VLAVVEQPGAAPAQLETEVARKMEDSMASLPGLRHLRTNITDGRVAVTAEFVLSKSLA
                     EAQLDVKDAIDKIRSQLPANVEEPQVTKINEGPGGPILTYALWNPALDEEALSWFTDD
                     VVAHAVMSVQGVTNFYRIGGVTREVQVMVDPVQMSALGVTASDISRALKQVQQEASGG
                     RGELGGAEQGVRTIATVKRAQDLAALPITLTDGRSLRLDQVAQVHDTIAERKQAVLIN
                     GTPAVGFQISRVKGYDEKTINAQMDRVMAELEKNHPGLETKLVRSVVKTTMKQYSGSM
                     HMLIEGAILAVVVIWWFLRDWRATVLGAAALPLSIIPAFGVMYLADFSLNTITLLGLA
                     VVVGI"
     gene            complement(1189..2307)
                     /locus_tag="VFX11_15915"
     CDS             complement(1189..2307)
                     /locus_tag="VFX11_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940165.1"
                     /translation="MNAFRLSSLLFCAVLAGCSQPSDQAISAPKSAMTVTTAQVQLQT
                     MPQAAEAQGQIAPWQEAIISARVSGLTLTDLQVQVGDRVKRGQILARFDARSVNAELT
                     QARANLAQATANAVQAAANRDRTLRMQGKGAVSEQEQQLAQTQAEMAEAQRQMAAAQV
                     TTQEIRLQDCEVRAVDDGVISARNATLGQVAQAGTELFRLIRQERLEWQAELNSQQMA
                     RVQPGQSVVINLPGGNQVSGTVRQLAPSMNNNSRLGLAYVDLQPGSAARAGMYVSGMI
                     DLDQRESLVVPAEAVVLRDGRSSVFRMGDDKKVIQIPVETGRRNGTMIEIAQGVAAGD
                     RIAVRGAGFLADGDLVEQVADRELGVQASNLPDSEAVQ"
     gene            2482..3231
                     /locus_tag="VFX11_15920"
     CDS             2482..3231
                     /locus_tag="VFX11_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEX4940166.1"
                     /translation="MNIRTLARLFSACALPVFLMPGAACADFTSAAPDACAVNLNSPI
                     QNFCEATRNTLWRGAKPDTAEAAWLIRNGVRTIVNLELLNDDRDTLTSVHLEEQADDR
                     ATLAETRLPTLEEHEIHYYRVRDWEPLVIVAPWLTDERVATFLAVAAQAPKPLYVHCR
                     SGQNRTGVMVAAYRVILESEGDKAAIEKAVAEMERYRGFWFDSDAAYIRGLTPERQQA
                     IRDRMQELTPTVASKREALIRCREGKCTAVD"
     gene            3314..>3595
                     /locus_tag="VFX11_15925"
     CDS             3314..>3595
                     /locus_tag="VFX11_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEX4940167.1"
                     /translation="MKIGEIAKASGVGIDTLRYYEKEGLLRPAGRSDSGYRHYDRQAV
                     QQMQFILKAKALGFSLQEIRELLSLRIDREHQPCSSVKDLAQAKLKNIET"
BASE COUNT          660 a         1101 c         1125 g          709 t
ORIGIN      
        1 ggatgcccac cacgacagcc aggcccagca aagtgatggt gttgagcgag aagtctgcca
       61 ggtacatcac cccaaaggcg ggaatgatgg acagtggcag cgccgcagca cccagcacgg
      121 tggcacgcca gtcacgcagg aaccaccaga tcacgacgac ggccagaatg gcgccttcga
      181 tcagcatgtg catcgagccg gaatattgct tcatggtggt cttcaccacc gaacgtacca
      241 gcttggtctc cagccccgga tggttttttt ccagttccgc catcacccga tccatctgcg
      301 cattgatggt cttctcgtcg tagcccttga cgcgggaaat ctggaatccc actgccggtg
      361 tgccattgat gagcaccgcc tgtttgcgtt cggcgatggt gtcgtgcacc tgggcgacct
      421 gatcgaggcg cagggagcgg ccgtcggtga gcgtaatggg cagtgcagcc agatcctggg
      481 cccgctttac ggtagcgatt gtgcgcacgc cctgttcggc gccgcccagc tcgccgcgcc
      541 cgccggacgc ttcctgctgc acctgtttaa gtgcgcgcga gatatcggat gccgtcacac
      601 ccagcgcgct catctgcacc gggtccacca tcacctgcac ttcccgcgtc acaccgccga
      661 tgcgatagaa attggtgacg ccctgcaccg acatgaccgc atgcgccacc acgtcgtccg
      721 taaaccagga cagggcctct tcatccaggg cgggattcca gagtgcgtag gtcagaatgg
      781 ggccgccggg gccttcgttg atcttggtga cctgtggctc ctccacgttg gcgggcagct
      841 ggctgcggat cttgtcgatc gcatctttca catccagctg cgcttccgcc agtgatttgg
      901 aaagcacgaa ctcggcggta acggcaaccc ggccatcggt aatgttggtg cgcagatgac
      961 gcaggccggg cagcgacgcc atcgagtctt ccatcttgcg cgccacttcc gtttccagct
     1021 gtgcgggggc cgcaccgggc tgctcgacca cggccagaac cgccggataa tccatgtcgg
     1081 gaaagttctg gatgttgagc tggttgaatc cccagatacc tgccagcgtc aggaagaaaa
     1141 acagcaggat gttgggaatg ggattgtgga tggaccaggt tgccagattc attggacggc
     1201 ctcggaatca ggcaggttgg acgcttgcac acccagttcc cggtctgcga cctgttccac
     1261 caggtcgcca tcggccagga agcctgcgcc gcgcacggca atccggtcac cggccgccac
     1321 gccctgtgcg atttcgatca tggtgccgtt acggcggccg gtttccaccg gaatctggat
     1381 gacctttttg tcgtcgccca tccggaacac cgagctgcgg ccgtcacgca gcaccaccgc
     1441 ttctgccggc accaccagcg attcccgctg gtccagatca atcatgccac tgacatacat
     1501 gccggcccgt gccgcactgc cgggttgcag atccacatag gccagcccca gacgggaatt
     1561 gttgttcatg gaaggggcga gttggcgcac ggtgccgctt acctggttgc caccgggcag
     1621 gttgatcacg acagattgcc cgggctgcac ccgcgccatc tgctgggaat tgagttcggc
     1681 ctgccactcc agccgttcct ggcggatcag ccggaacagt tcagtaccgg cctgggcaac
     1741 ctggccgaga gtggcgttgc gggccgagat cacaccgtcg tccaccgcgc gcacttcgca
     1801 atcctgcagg cggatttcct gggtggtgac ttgggcagcg gccatctggc gctgggcttc
     1861 cgccatttct gcctgggtct gggccagttg ctgctcctgc tcgctgacgg cgcccttgcc
     1921 ctgcatgcgc agggtgcggt cgcggttggc ggctgcctgc acggcattgg cggtggcctg
     1981 ggccagattg gccctggcct gggtcagttc agcgttgaca ctgcgtgcgt cgaaccgggc
     2041 cagaatctgg ccgcgcttca cccggtcacc cacctgaacc tgcaagtctg tcagcgtgag
     2101 tccgctcacc cgggcactga tgatggcttc ctgccagggg gcgatctggc cctgggcttc
     2161 cgccgcctgc ggcatcgtct gcagctggac ctgtgcagtg gtaacggtca tcgctgattt
     2221 gggcgcggat atggcctggt ccgatggttg actgcaaccg gcaagcacgg cgcagaaaag
     2281 cagactgctg aggcggaagg cgttcattca gtactccgga tacacaactg tgagggggca
     2341 ggaaatgcct gattttggtt atttaaccaa atactaccct gttttggtta aataaccaaa
     2401 tggtatccgg gcgggggcgg cgctggcggc caggcggaca ttgggttata tctgcacgca
     2461 attcctctgc cggacctgtc catgaatatt cgcacgctcg cacgcttgtt ttctgcctgc
     2521 gcactccctg tcttcctgat gcccggcgcc gcctgcgccg atttcaccag cgctgcgccg
     2581 gatgcctgtg cagtgaacct gaattccccg atccagaatt tctgcgaggc aacccggaac
     2641 acactctggc gcggtgccaa gccggatacg gcggaagcgg cctggctgat ccgcaatggc
     2701 gtccgcacca tcgtcaatct ggaattgctg aatgatgacc gcgacacgct gaccagcgtg
     2761 catctggagg aacaggctga tgatcgcgcc acgctggcgg agacccggct gccgacactg
     2821 gaggagcacg aaatccacta ctaccgcgtg cgtgactggg agccgctggt gatcgtggcg
     2881 ccctggctga ccgacgagcg cgtggccacg tttctggccg tggcggcaca ggcacccaag
     2941 ccgctgtatg tgcattgccg ttccggccag aaccgcaccg gggtgatggt ggcggcgtac
     3001 cgggtgatcc tggaaagcga gggtgacaag gcggctatag aaaaggcagt tgccgagatg
     3061 gaacgctatc gcggtttctg gttcgattcg gatgcagcct atattcgtgg cctgaccccc
     3121 gagcggcagc aggcgatccg cgaccggatg caggaattga cgcccaccgt ggccagcaaa
     3181 cgagaggcgc tgatccgttg ccgggaaggt aaatgcactg cagtggatta gggagcccgt
     3241 aacggttgac tctggtcctt gctccagggt ttatactgct ttcactgatt acggggcgtc
     3301 attcgaggtg cacatgaaaa tcggtgaaat cgccaaggcc agcggggtgg gtatcgacac
     3361 gctgcgttat tacgagaagg aaggcctgct gcggcccgcc ggacgttcgg attccggtta
     3421 ccggcattac gatcgccagg cagtgcagca gatgcagttc atcctgaaag ccaaggcgct
     3481 gggtttcagt ctgcaggaaa tccgtgagct gctgtcattg cgcatcgatc gcgagcatca
     3541 gccctgttcc agcgtgaaag atctggcgca ggccaagctg aaaaacatcg agacg
//
LOCUS       DASUEG010000846         4297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_298274, whole genome shotgun sequence.
ACCESSION   DASUEG010000846 DASUEG010000000
VERSION     DASUEG010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298274"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VFX11_15930"
     CDS             complement(<1..837)
                     /locus_tag="VFX11_15930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX4940168.1"
                     /translation="MTQTAQSVLEHVFGYSAFRGPQQAIVDTLIDGRDALVIMPTGGG
                     KSLCYQVPAIVRPGCGVVISPLIALMQDQVDALQAAGVKAGFLNSSLSAEQANATEQA
                     LLKGELDLLYVAPERLIQPRCLHLLKRSRIALFAIDEAHCVSQWGHDFRSDYLQLSLL
                     HEQFPAIPRIALTATADARTRDEIAARLNLQNARHFISGFDRPNIQYRIQQKDKPLDQ
                     LLHFLRTEQKDNAGVVYCLSRNKVEKVAEYLCDKGFNALPYHAGLSADIRATHQRRFL
                     RED"
     gene            complement(857..1942)
                     /locus_tag="VFX11_15935"
     CDS             complement(857..1942)
                     /locus_tag="VFX11_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4940169.1"
                     /translation="MTPQWIAGPDCPLTRHGEIPRFRQGLYRLTPNAYAWMVPNGSWG
                     ETNIGLVECNGKSVLIDTCWDLHFTRQMLGAAAPVVQRSPIDIVINTHADGDHCWGNQ
                     LFRDREIIATQACVHQMHHMAPRSLTALQKGGRILRHLPAAGLDCFGHYMSAMFQPYD
                     FSGVHITGPQQGFCHEKCVTVNGVELVLMEVGPGHTDGDALVYVPAESVVYAGDIAFI
                     GVTPVMWSGPLDNLTAGLRKLLALKAGVIVPGHGPFASPADIQAILDYWEFAQEQLHA
                     RFQRDLTPFEAARDLVLSPTFQATAFAHWDSPERMVTNAHTLYRHWGARLFNLPGKLG
                     IMDIMRQQAILAFALPEATPRRLRHPD"
     gene            complement(1947..2981)
                     /locus_tag="VFX11_15940"
     CDS             complement(1947..2981)
                     /locus_tag="VFX11_15940"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4940170.1"
                     /translation="MQLKRFRRGEQFLLAVNLAGPNPGAANGGEQWIQLDQLPGLEHT
                     GSDMLAVLAQWPQLKPLLDQALQNADGNNLPAVPTDAQPVAAFAPLSLRDFMLSEQHA
                     VNAARGMVRHFMPRVFPLVKGFEKISGRVFPALQPKPLWYQQPLYYFSNHLNVAGDDA
                     VIDWPAYTRYLDYELELAFVLAQPLKNATPQQALAAIGGFMVLNDVSARDVQLAEMRS
                     GFGPQKAKHFVNALSYTVATADAVMPRVNQLRAEARINGAVVCKTTTSNLQHSIADML
                     VHASRDEQLHPGEVFGLGTLPGGCALENDHWLQPGDSIELWIEGVGHLRNRIAAPDAA
                     SATHNAAKGR"
     gene            complement(2994..3635)
                     /locus_tag="VFX11_15945"
     CDS             complement(2994..3635)
                     /locus_tag="VFX11_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4940171.1"
                     /translation="MPNPIKEPLQARSRATANQVLDALYDLLRQKDLAAISIQELART
                     SGASVSSIYARFQDKHAMVLGLHERLTERLTQELTAIAQRPFAESSLQQVVETVLTDF
                     LAFARRHQHVYRAAILSADPLIYARVVGQIRLASQLCFSWLQAHPLVPADDLEQRVDF
                     AVRALVATLQQTWVMGTQSPSRFELSDAELVQQLGRMATAYLFWSGDPDGKQD"
     gene            3744..>4297
                     /locus_tag="VFX11_15950"
     CDS             3744..>4297
                     /locus_tag="VFX11_15950"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4940172.1"
                     /translation="MNAPDETLHTLAQLCKGTGDPLRLEILRALKTDSFGVLELCDIF
                     AIKQPAMSHHLKVLANAGAVTTRREGNSIFYRRELADLSQPWGQLLAALFSAVDQIAL
                     SDTTARNIAQIKHEREEQAREFFDRHAEQFREQQDLIAPFDQYVDAATDLLLQTEPDQ
                     QQRVLEIGPGEGEFLPTLAGIFHSV"
BASE COUNT          848 a         1332 c         1303 g          814 t
ORIGIN      
        1 gtcctcgcgc aggaaacggc gctggtgagt ggcgcggatg tccgcactca aaccggcgtg
       61 atacggcagc gcgttgaagc ctttgtcgca cagatattcc gctaccttct ccaccttgtt
      121 gcgcgacagg caatacacca cgccggcgtt gtccttctgc tcggtgcgca gaaaatgcag
      181 cagctggtcc agcggtttgt ctttctgctg gatgcggtat tgaatgttgg ggcgatcaaa
      241 accgctaatg aagtgccggg cgttttgcag gttcaggcgt gcggcgattt cgtcgcgggt
      301 gcgggcgtcg gcagtggcgg tcagcgcaat acgtggaatc gcgggaaact gttcatgcag
      361 caggctcagc tgcagatagt cggaacgaaa atcgtgaccc cattgcgaca cgcagtgggc
      421 ttcgtcgatg gcaaacaggg cgatgcggga acgcttgagc agatgcaggc agcggggctg
      481 gatcaatcgt tccggcgcca cgtacaacag atccagttcg cctttcagca acgcctgttc
      541 ggtggcattg gcctgttccg ccgacagcga ggaattgaga aagccggctt tcacacccgc
      601 cgcctgcagc gcatccacct ggtcctgcat cagcgcgatc aagggcgaaa tcaccacgcc
      661 gcagcccggg cgcacaatgg cgggcacctg gtagcacagc gacttgccgc cgccggtggg
      721 catgatcacc agcgcgtcgc ggccatcgat cagggtatcg acgatggcct gctgggggcc
      781 gcgaaaggcg gagtagccga agacgtgttc aaggacggac tgggctgtct gggtcatggg
      841 gccgcaaata aagtggtcaa tctggatgcc gcaggcgccg gggtgtcgcc tcgggcagcg
      901 caaacgcgag aatggcctgc tgccgcatga tgtccatgat acccagcttg cccggcaggt
      961 tgaacagccg cgcgccccaa tggcgatata aagtatgggc attggtaacc atgcgctccg
     1021 gcgaatccca gtgcgcgaac gcagtggcct ggaacgtggg gctcaaaacc agatcgcggg
     1081 cagcctcgaa gggcgtcaga tcgcgctgga aacgggcatg cagctgttcc tgggcgaact
     1141 cccagtaatc cagaatggcc tggatatccg ccggactggc gaatgggccg tggcccggca
     1201 cgatcacccc ggccttcagc gccagcagct tgcgcaggcc agccgtcagg ttgtccagcg
     1261 ggccgctcca catcaccggc gtcacgccga taaacgcaat atcgccggca tagacgacgc
     1321 tttccgccgg cacatacacc agcgcatcac catcggtatg gccgggcccc acttccatca
     1381 gcaccagctc cacgccgttg acggtcacgc atttctcgtg acagaaacct tgctggggac
     1441 ccgtaatgtg cacgccggaa aaatcgtagg gctggaacat ggcactcatg taatggccga
     1501 aacagtcgag accggctgcg ggcagatgac gcaggatcct gccgcccttc tgcaatgcgg
     1561 tgagcgagcg cggcgccatg tgatgcatct ggtggacgca ggcctgcgtg gcgatgatct
     1621 cgcggtccct gaacagctgg ttgccccagc agtgatcacc gtcggcatgg gtgttgatga
     1681 cgatgtcgat cggcgaacgc tgcactaccg gtgcggcggc acccagcatt tgccgcgtga
     1741 aatgcagatc ccagcaggtg tcgatcagca cgcttttgcc gttgcactcc accaggccga
     1801 tattggtttc gccccaggaa ccattgggca ccatccaggc gtaggcgttc ggcgtcagcc
     1861 ggtacaagcc ctggcggaag cggggaattt ccccgtgccg ggtgagcggg cagtcggggc
     1921 cggcgatcca ttgcggtgtc atggctttac ctgcctttgg ctgcgttatg ggtagcggaa
     1981 gccgcgtctg gcgccgcgat gcggttacgc aagtggccga ccccttcgat ccacaattcg
     2041 atgctgtcac ccggttgcaa ccagtgatcg ttttccagcg cacaaccacc cggcagcgtg
     2101 cccagcccaa acacttctcc tggatgcagc tgttcgtccc gcgaggcgtg taccaacatg
     2161 tcggcaatgg aatgctgcag attggatgta gtggttttac atacaacggc gccgttgata
     2221 cgcgcctccg cccgcagctg atttacccgt ggcatcaccg catcggccgt cgccaccgtg
     2281 tacgacagcg cattgacaaa atgcttcgct ttctgcggac cgaaaccgct gcgcatttcc
     2341 gccagctgca catcgcgcgc actgacatcg ttcagcacca taaatccacc aatggccgcc
     2401 agcgcctgtt gtggcgtggc atttttcagc ggctgcgcca gcacgaacgc cagttccagt
     2461 tcgtaatcca gatagcgcgt ataagcgggc cagtcgatca ccgcgtcgtc gcccgccaca
     2521 ttcaggtgat tgctgaaata gtagagcggc tgctgatacc agagcggttt gggttgcagt
     2581 gccggaaaca cccggccact gattttttca aaccctttca ccagcggaaa aacgcgtggc
     2641 atgaagtgcc gcaccatgcc ccgcgctgca tttaccgcat gctgttccga cagcatgaag
     2701 tcccgcagcg acagtggcgc gaatgccgcc accggttgcg cgtcggtggg tactgccggc
     2761 agattatttc cgtctgcatt ttgcaatgcc tgatccagca ggggtttcag ttgcggccat
     2821 tgcgccagca ctgccagcat gtcgctgccg gtgtgttcca ggccaggcaa ttgatccagc
     2881 tgaatccact gttccccgcc attagccgcg ccagggttcg gcccggccag attcaccgcc
     2941 agcagaaatt gctcaccgcg tcggaaacgt ttcagctgca tggttttgct ccttcaatcc
     3001 tgtttgccat ccggatcacc gctccaaaac agataggccg tcgccatgcg gcccagctgc
     3061 tgcaccagtt cggcgtcgct caattcaaac cgcgatggcg attgcgttcc catcacccag
     3121 gtctgctgca aggtggccac cagagcgcgc acggcaaaat ccacccgctg ctccagatcg
     3181 tcggccggca ccagcggatg cgcctgcagc cagctgaaac ataactgtga cgccaggcgg
     3241 atctgtccca ccacccgcgc gtaaatcagc gggtcggcgg acaggatcgc cgcccggtac
     3301 acatgctggt gacgacgggc aaaggcgaga aaatcggtca gcactgtttc caccacctgc
     3361 tgcaatgaac tttccgcaaa gggccgttgc gcgatcgcgg taagttcctg ggtgaggcgc
     3421 tccgtcagcc gctcgtgcag ccccagcacc atggcgtgct tgtcctggaa gcgggcgtag
     3481 atggaactga cggacgcccc ggacgtccgg gccagttcct ggatgctgat ggcggccaga
     3541 tccttctgcc gcaacaggtc atacagggcg tccagcacct ggttggcagt ggcgcggctg
     3601 cgcgcctgca ggggttcttt gatgggattc ggcatgcagg catgctgcca caaactggaa
     3661 ttggatttca attccagttt gtggcagccc tctatatcaa aaacttttga tatagagttt
     3721 tcaggctgat acactccgcc cccatgaacg cgcccgacga aaccctgcac accctcgccc
     3781 agctctgtaa aggcaccggc gacccgttgc gactggaaat cctgcgcgcc ctgaagaccg
     3841 attctttcgg cgtgctggag ctgtgcgaca tcttcgccat caagcagccc gccatgagcc
     3901 accacctgaa ggtgctggcc aacgccggcg cggtgaccac ccggcgcgag ggcaactcga
     3961 ttttctaccg gcgcgaactg gcggacctga gccagccctg gggtcagtta ctggcagccc
     4021 tgttcagtgc ggtggaccag attgcgctga gcgatacgac ggcccgtaac attgcgcaga
     4081 taaaacatga acgggaagaa caggcgcggg aatttttcga ccgccatgcg gaacagtttc
     4141 gcgaacagca ggatctgatt gccccgtttg accagtatgt cgacgccgcc accgatctgt
     4201 tgctgcaaac cgagccggac cagcagcaac gggtgctgga gatcggcccg ggcgaaggcg
     4261 aattcctgcc gacgctggcg ggcattttcc acagcgt
//
LOCUS       DASUEG010000847         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1425082, whole genome shotgun sequence.
ACCESSION   DASUEG010000847 DASUEG010000000
VERSION     DASUEG010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425082"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFX11_15955"
     CDS             <1..621
                     /locus_tag="VFX11_15955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4940173.1"
                     /translation="KVLKTADYLTPRHRDALETVQRNAHHLLGLISDILDLSKIEAGR
                     MDVDHDAVDLLPFLEQARSEFTQMAKAKGLAFALVQPPTLPVLHTDRGKLVQICNNLL
                     SNAIKYTERGTVTLEVATLQHDEIGAAVALHFHDTGIGIHPEDQQKLFKEFGRAREAR
                     EKRIQGTGLGLLITAQLVGLLGGRINFHSVHGEGSTFSVILPAPTV"
     gene            758..1243
                     /locus_tag="VFX11_15960"
     CDS             758..1243
                     /locus_tag="VFX11_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX4940174.1"
                     /translation="MPVRPVTEFLDHNNVNYHSYNHPPAVTAQEIAQSAHIPGAQLAK
                     TVIVWGDGELAMAVLPAHQRINGNLLKMIMKVRDLRLASEDEFRERFPQCETGGMPPL
                     GNLYGMKVYMERALLTDDWIAFNAGTHTEVIKMDTGVFKRLVKPVLCSFARVQTLAAA
                     H"
     gene            complement(1316..2047)
                     /locus_tag="VFX11_15965"
     CDS             complement(1316..2047)
                     /locus_tag="VFX11_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4940175.1"
                     /translation="MFLKQMAGALALGGLVLPAFAAEKGCEGLPTHAQLKAALTDARK
                     AQNAGFDLDMWGTLVNRDGVVCAVAFTGKDRGDQWPGSRVISAQKAYTANAFSLPGLA
                     LATANLYSAVQPGGSLYGLQHSNPVDTDAAYEGKAEKFGQTDDPMVGERVGGVNVFGG
                     GLGLYNAQGKLIGALGVSGDSSCADHFIAWRARHSLKLDYVPAGVVNGVDQISFSGGW
                     SQPHCKDAAAEDATLATLPATAKKP"
BASE COUNT          458 a          701 c          628 g          405 t
ORIGIN      
        1 aaagtgttga agaccgccga ctacctgaca ccccgccacc gcgacgcact ggaaaccgtg
       61 cagcgcaacg cccaccacct gctcggactg atcagcgaca ttctggatct gtcgaagatc
      121 gaagccggcc gcatggatgt ggatcacgac gcggtggacc tgctgccctt cctcgaacag
      181 gcccgcagcg aattcaccca gatggcgaaa gccaaaggac tggcgtttgc actggtgcag
      241 ccacccacac tgccggtgct gcataccgac cgcggcaagc tggtgcagat ctgcaacaac
      301 ctgctgtcca acgccataaa atacacagaa cgcggcacgg tgacgctgga agtggcaacg
      361 ctgcagcacg acgagatcgg cgccgccgtg gcgctgcatt ttcacgatac cggcatcggc
      421 attcacccgg aggaccagca gaaactgttc aaggaattcg gccgggcgcg ggaagcacgg
      481 gaaaagcgca tccagggcac cggcctgggg ctgctgatca cggcgcaact ggtggggctg
      541 ctgggcgggc gcatcaactt ccacagcgtg cacggggaag gcagcacatt cagtgtgatc
      601 ctgcccgcgc caacagtctg aggcactttt gacagcgcct taaatccaat tggaagattc
      661 gatattcccc ttcgttcttt tcgctccccc gcgcgcggac ctatgctttt tctgcacctt
      721 ggcaacagtg gatgcgatga cgcacgggag gtgtgccatg ccagtcagac cagtgaccga
      781 atttctcgac cacaacaacg tgaattacca cagctacaat catccgccgg cggtgaccgc
      841 ccaggagatt gcgcaatcag cccacatccc cggtgcacaa ctggccaaga ccgtgatcgt
      901 ctggggcgat ggtgaactgg cgatggcagt gttgcccgca catcagcgca tcaacggcaa
      961 cctgctgaag atgatcatga aagtacgcga cctgcgtctg gccagcgagg atgaattccg
     1021 cgaacgcttt ccccagtgcg aaaccggtgg catgccgccg ctgggcaatc tgtacgggat
     1081 gaaggtgtac atggaacgcg cgctgctgac cgacgattgg atcgccttca acgccggcac
     1141 ccacaccgaa gtcatcaaga tggataccgg cgtattcaag cgactggtga aaccggtgct
     1201 gtgttccttt gcgcgggtac aaacgctggc ggccgcgcac tagccggcgt cgtcaggttc
     1261 acgcaagaaa aaggccggtg tgaccggcct tcgtggctgt tttgttgaat gaagctcagg
     1321 gctttttcgc cgttgccggc aatgtggcca gtgttgcatc ttccgctgcc gcatccttgc
     1381 agtgcggctg gctccagcca ccgctgaaac tgatctggtc aacaccgttc accacaccag
     1441 caggcacata atccagcttg aggctgtggc gggcgcgcca cgcgatgaaa tgatcggcgc
     1501 aggagctgtc gccggacaca cccagcgcgc caatcaactt gccttgcgcg ttgtacagac
     1561 ccagcccgcc gccgaacacg ttcacaccgc caacacgctc gcccaccatg ggatcatcgg
     1621 tctggccgaa tttttccgcc ttgccttcat aggccgcatc ggtgtccacc ggattgctgt
     1681 gttgcaggcc gtacaggctg ccgcccggtt gcaccgcgct gtacagattg gccgtggcca
     1741 gtgccaggcc gggcagactg aaggcgttgg cggtgtaggc tttctgcgcg gaaataaccc
     1801 ggctgcccgg ccactggtcg ccgcgatcct tgccggtgaa tgccactgca cacaccacgc
     1861 catcgcggtt caccagcgtg ccccacatat ccagatcaaa gcccgcgttc tgcgccttgc
     1921 gcgcgtcagt gagcgccgct ttcagttgcg cgtgcgtggg cagaccttcg cagccttttt
     1981 cggcggcaaa cgccggcaat accaatccgc ccagcgccag tgcgccagcc atctgtttca
     2041 aaaacatagt caagctcccg tgaggtcaaa acaggcagtg ctgccactgc cacaaaaatc
     2101 aggccagtcc ggccgttttc atccactcat aacagtcgcg gaacatttca tccagcgagc
     2161 aggtggtgaa gcccagttcc cgttcggctt tt
//
LOCUS       DASUEG010000848         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1440971, whole genome shotgun sequence.
ACCESSION   DASUEG010000848 DASUEG010000000
VERSION     DASUEG010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440971"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2621
                     /locus_tag="VFX11_15970"
     CDS             <1..2621
                     /locus_tag="VFX11_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX4940176.1"
                     /translation="FIENRRLLDADHPQVNPAVDWGRFIKAAVFKAGDAINIKTPSMG
                     GSTLATQIEKYRHSDDGITSSIEDKLVQMASATVRVYQQGPDTMAARRKLVLDYLNTV
                     PLSAAPGYGEVNGIGDGLFVWFGSDFDRINQLLSGPDLTGAELVEQGIAMRQIIALMI
                     AHRRPSYYLAQSRSDLDQLTDSYLRLLGRKGAIGQELMQTALAQKTVFRNFRADPAVV
                     PQETNKGVNVVRNRLVSSLDVSLYDLDRMDLTATTTLQGDLQDAVTAHLRKLHDDEFA
                     KQNGLMGEYLLKPGQAKHINYSFTLFERTPNGNQVRVQTDNTDNPFDINEGSKLELGS
                     TAKLRVLSTYLEVMAEIHQRYQNQGADSLRKLREDTTDVLTGWVLEQLIARPDLTLPE
                     LLQASLDRTYSADPGERFFTGGGIHTFNNFKKEDNGRVPTVRESLQESINLPFVRMLR
                     ELVNYTTYQKWSGENLVRLNDSDPRRKEVLTKFVDRESTVFLSRFWVKYQGLTQQERM
                     DAFLTGLKPTPVRLAVVHRFLFPEADLPTFSAFLNDRLPGEALASKTLSNLYSRYKPG
                     SYNLQDLGYLARVHPLELWLLGYLQQADKPSFSKALEASRDERQQVYSWLLRTKAKNA
                     RDTRVRTMLEVEAFSDIHRRWKALGYPFDHLVPSLATALGSSGDRPAALAELMGVILN
                     GGERMPVYRLDKLVFGAGTPYEARLSQQPMSPVQVMNPDVADALKGALGEVVNNGTAR
                     RLQGAYHKADGSPLNMGGKTGTGDNRLVSMGAGGQRTKGRALSRTATLVFYLGNDHFG
                     TLTAFVSGSGAGDFRFTSALPAQTLKGMAPILEPYLQQVETPAELAPEMWHDGFEEPG
                     DPAQAAIESDELSPAE"
     gene            2709..>3147
                     /locus_tag="VFX11_15975"
     CDS             2709..>3147
                     /locus_tag="VFX11_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005894082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4940177.1"
                     /translation="MGAQWKAKGRTESAAAKSRIFTKLTREIMVAARSGADPATNSRL
                     RIAIEQAKKASMPKDTLERAIKKGAGLLDEVVHYEHVTYEGFAPHRVPVIVECLTDNN
                     NRTAQSIRVLFRKGQLGTSGSVSWDFKYLGMIEATPDSPDADPE"
BASE COUNT          638 a          973 c          975 g          561 t
ORIGIN      
        1 tgttcattga aaaccgccgc ctgctggatg ccgatcatcc tcaggtgaat ccggccgtgg
       61 attgggggcg cttcatcaag gccgctgtgt tcaaggccgg cgatgccatc aacatcaaga
      121 cgccgtccat gggcggcagt accctggcca cccagatcga gaaataccgc cactccgacg
      181 atggcatcac ctcatccatc gaggacaagc tggtgcagat ggcctccgcc accgtgcggg
      241 tgtaccagca ggggccggat accatggctg cccgtcgcaa gctggtgctg gattacctga
      301 ataccgtgcc gctgtctgcc gcgccgggtt atggcgaagt gaatggtatt ggcgacggcc
      361 tgttcgtctg gttcggttct gacttcgacc gcatcaacca gctgctgagc gggccggacc
      421 tgacgggagc ggaactggta gagcagggca tagcgatgcg ccagatcatt gcgctgatga
      481 tcgcccaccg tcgtccttcc tattatctgg cgcagagccg cagcgatctg gaccagctca
      541 ccgacagcta cctgcgcctg ctgggtcgca agggcgccat tggccaggaa ctgatgcaga
      601 ccgcactggc gcagaaaacc gtattccgta atttccgtgc cgacccggcc gtggtgccgc
      661 aggaaaccaa caagggcgtc aacgtggtgc gcaaccggct ggtgtcgtcg ctggatgtgt
      721 cgctgtacga cctggatcgc atggacctga ctgccaccac cacgctgcag ggggatctgc
      781 aggacgccgt tactgcccat ctgcgcaagc tgcatgacga cgagtttgcg aagcagaatg
      841 gactgatggg ggagtatctg ctcaagccgg gccaggccaa acacatcaat tacagcttca
      901 ccctgttcga gcgcacgccc aacggcaacc aggtgcgggt gcagaccgac aacaccgaca
      961 acccgttcga tatcaatgag ggtagcaagc tggagctggg gtccactgcc aagctgcggg
     1021 tgctgtccac gtatcttgaa gtcatggcgg aaatccacca gcgctatcag aaccagggtg
     1081 cggactccct gcgcaagctg cgggaagaca ctaccgacgt gctgaccggc tgggtgctgg
     1141 aacagctgat cgccaggcct gatctgaccc tgcccgagct gttgcaggca tcgctggacc
     1201 gcacttactc ggccgatccc ggcgagcgct tcttcaccgg cggcggcatt cataccttca
     1261 acaacttcaa gaaggaagac aacggccgcg ttcccaccgt gcgcgaatcc ctgcaggaat
     1321 ccatcaacct gccgttcgta cgcatgctgc gggaactggt gaactacacg acctaccaga
     1381 agtggagcgg tgagaatctg gtgcgcctga acgattcgga cccgcgcagg aaggaagtgc
     1441 tgaccaaatt tgtcgaccgc gagagtactg tgttcctgtc ccgcttctgg gtgaagtacc
     1501 agggcctgac ccagcaggag cgcatggatg cctttctgac cggattgaag ccgacgccgg
     1561 tacggctggc ggtggtgcac cgttttctgt ttccggaagc ggacctgccg accttctcgg
     1621 cattcctgaa cgatcgcctg cccggtgagg ccctggcgtc gaagacgctg tcgaacctgt
     1681 acagccgtta caagcctggc agctataacc tgcaggacct gggttacctg gcgcgggtgc
     1741 atccgctgga actctggctg ctggggtatc tgcagcaggc cgacaagccc agtttcagca
     1801 aggcactgga agccagccgg gacgaacgcc agcaggtgta cagctggctg ctgcgcacta
     1861 aagccaagaa cgcacgcgac acccgtgtcc gcaccatgct ggaggtggaa gcgttctccg
     1921 acatacaccg gcgctggaaa gcactgggct atccgttcga tcatctggtg ccgtctctgg
     1981 ccactgccct gggcagttcc ggcgaccggc cggcggccct ggcggaattg atgggcgtga
     2041 tcctgaatgg cggcgaacgc atgccggtgt accggctcga caagctggtg tttggcgccg
     2101 gcactccgta cgaggcgcgg ctgtcacagc agcccatgag cccggttcag gtgatgaatc
     2161 ccgatgtggc cgatgccctc aagggtgcgc tgggcgaagt ggtgaacaat ggaaccgccc
     2221 gccgtctgca gggggcctat cacaaggccg acggcagccc tctcaacatg ggtggcaaga
     2281 ccggcaccgg cgacaaccgt ctggtgtcca tgggcgccgg cggccagcgc accaagggcc
     2341 gcgccctcag ccgcactgcg acgctggtgt tctatctggg caatgatcat ttcggtaccc
     2401 tgacagcctt cgtatcgggg tccggtgccg gtgatttccg ttttacctcc gccctgccgg
     2461 cccagaccct caagggaatg gcgcccattc tggagcccta tctgcagcag gtcgagacac
     2521 cggccgagct ggccccggaa atgtggcatg atggttttga ggagccgggc gatccggcac
     2581 aggcagccat cgaatccgac gaactgtctc ctgcggagtg aagcgccggt ccacttcatt
     2641 gtggccggcc gtccctttat actcccgcac aattaaatcc ccactcaaaa taagtcgaga
     2701 gaaccgctat gggcgcacag tggaaagcca agggtagaac cgaatccgcc gccgcaaaaa
     2761 gccgcatctt taccaaactc acccgggaaa tcatggtggc ggcacggtcg ggcgccgatc
     2821 cggccacgaa ttcccgcctg cgaattgcga tcgagcaggc caagaaggcg tcgatgccga
     2881 aggacaccct ggagcgcgcg atcaagaaag gcgccggtct gctggatgaa gtggtgcact
     2941 acgaacacgt gacgtacgaa ggtttcgcgc cgcaccgggt gccggtgatc gtggagtgcc
     3001 tgactgacaa caacaaccgc accgcacaga gcatccgcgt actgttccgc aaggggcagc
     3061 tgggtacgtc cggttcggtg tcctgggatt tcaaatacct gggcatgatc gaagcgaccc
     3121 cggattcccc cgatgccgac ccggaaa
//
LOCUS       DASUEG010000849         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_563341, whole genome shotgun sequence.
ACCESSION   DASUEG010000849 DASUEG010000000
VERSION     DASUEG010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563341"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VFX11_15980"
     CDS             complement(<1..121)
                     /locus_tag="VFX11_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX4940178.1"
                     /translation="MRIILLGAPGAGKGTQAQYITEKFGIPQISTGDMLRAAVK"
     gene            complement(408..1808)
                     /locus_tag="VFX11_15985"
     CDS             complement(408..1808)
                     /locus_tag="VFX11_15985"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4940179.1"
                     /translation="MAIAVSLQRYFERKGIVYKTHPHVRESSLSKLCFELGVDPAHVA
                     VPVLLQSARKAYLMAVLPLSHGLDMNRVEALLRREFTWLDDYAVSQRFLDVEPGAEPP
                     IPEPYDLPCIVDRSLMDVGRVFFRAGTHSCLASVDADTFQFLFSAYPKAVISNPMQTL
                     AEELIPSAAAGDADRIHQYLENMHRLPAMPAMALKIIQLVADTKTTAVDLAEAVELDP
                     SMAAQIVRYAASPYYGYRGRVNSVQDAITRVLGFDLVSNIALGIASGRAFKVPQEGPL
                     GLKAFWKHALYCAVVSQSLARKIDRPDLINPATAYLAGLLHNIGVLLVGHLFPPEFCL
                     LNKLASKHPEKPLHAIEKDMIGMGQARQLIALGHDQIGGFLMEKWRLPEEVVACCYHH
                     HDEHYNGPFHDYVRLIQLSNRLLAQRQIGDLGVPDSLVNGFGAHLISFNAAEAVFEKV
                     MELCPEIDSLADHIAA"
     gene            complement(2036..3319)
                     /gene="ccmI"
                     /locus_tag="VFX11_15990"
     CDS             complement(2036..3319)
                     /gene="ccmI"
                     /locus_tag="VFX11_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX4940180.1"
                     /translation="MTQLWVLMTLMALAGALMVVLPLLKHRAREELSGVAVNSLVYRD
                     RLQELETDLREGRVQADEFAQLKAELELTLLDDVNLSQQDQGPRSLAGKWLVWPLFAL
                     VPLAAFFLYWTEGYRKETGDWLAAQPRLEAMIPQMLAGDFAALEQQGVQLPDLIRGMQ
                     AHLQKKPHDARSWYLLGVSYMQMRMAEQAELAFSRALNLEPDNVDYVVGYTQVAIAQN
                     NGVLAPDMRQTLIRIIEQHPDNPKPYMTLGMAAFQGGDFNGAIDIWQQYIQRENADSR
                     AVQLLQRSVEVARKQLAQAGSTPVSDSSQPVAAAGKPLLNVTVDLAAEARSQLSASDT
                     LFVYAKAVNGPPMPLAVVKQPVGNWPVVVQLSDANAMTPMATLSKFPDVIVQARISST
                     GNAIPQSGDWIGPTQVVKLAGGSQDVSLQISSRMP"
     gene            complement(3316..3813)
                     /locus_tag="VFX11_15995"
     CDS             complement(3316..3813)
                     /locus_tag="VFX11_15995"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX4940181.1"
                     /translation="MKRLLLLVAALWLMMLGGLALATIDAYEFRTPEDEQRFRVLVEE
                     LRCPKCQNQNVADSNAGLSKDIKDRVFRLINEGKSNAEITDHMVERFGDFITYRPPVR
                     PLTWFLWFGPFALALLAALIILARKFRQQPAPVQVTPEQEARVQALLRQLEQSETGAK
                     QEESK"
     gene            complement(3816..4412)
                     /locus_tag="VFX11_16000"
     CDS             complement(3816..4412)
                     /locus_tag="VFX11_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX4940182.1"
                     /translation="MSVSALSLRKGLLFSLPLIGFALLVYFFGRAIGSDPSILPSTRI
                     DQPLPAFQLPTLEDPSRTVTAGEIKGPLLLNVWATWCPSCIVEHPVLVALSREGIRMV
                     GLNYKDEPQAARRYLEINGNPFELNIADVKGDFGLDLGVYGAPESYLIDAAGKIRYRH
                     VGVITEDVWRTLLWPKWQEMGGTHPGPAAADDSQKGGA"
     gene            complement(4412..>5686)
                     /locus_tag="VFX11_16005"
     CDS             complement(4412..>5686)
                     /locus_tag="VFX11_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX4940183.1"
                     /translation="GWWFWDPVENASFMPWLAGTALMHSLAVTEKRGVFKAWTVLLAI
                     LAFSLSLLGTFLVRSGVLTSVHAFAADPERGYFVLVFLVLMVGGSLLLFLLRAQALKS
                     ESRFDLYSREMFLLFNNVILICATGLVLVGTLYPILFDVLGWGKISIGPPYFNTVFVP
                     VSLLLLVFTGIGPLVNWKRHQMALLKRTLLQALVLALLLGALALWLFDAGWDWIAFLA
                     LSVCVWVWWITLSELLRKIRTAKAGFMAGVRRLSLSYWGMVIAHLGIAVTVAGITMTS
                     TFDVERDVRMAAGDRVNIGPYVFVFDGVRDVRGPNFQSTEGVVKVLHGDEQIATLRPE
                     KRRYFVRRDVMTEAAIDPGFTRDLYVALGEPLQDGSWAVRVYYKPYMRWVWGGGVIMA
                     LGGFIALSDRRYRLQIKSRVAGAVSSLAGGKA"
BASE COUNT         1259 a         1782 c         1574 g         1071 t
ORIGIN      
        1 ccttgaccgc cgcacgcaac atgtcgccgg tggaaatctg gggtatgccg aacttttccg
       61 tgatgtattg tgcctgggta cctttccctg caccgggcgc gcctaacaaa atgatacgca
      121 ttgaacgaga ctccatgatg gttgatttct aaaacggatc gggaacgaaa gagttctcgc
      181 gcaacgctgc tctttcgttc gaggattcta gcaaattcag cttggtcgca gatggcggcc
      241 ggaatactgc atcggagatt ccggcatgga aaagaagggc caaacataca catgaaccgg
      301 accggttaca accccgctgg cggcggcgat ataactaatt gttttggcgg gatttttgaa
      361 aacagctgcg ggaaatggcg ggcaggccgg aatgcctgcc cgcgccatca tgcggcaatg
      421 tggtcggcga gactgtcgat ttcggggcac agctccatta ctttttcgaa cacagcttcc
      481 gcggcattga acgaaataag atgggcgcca aagccgttga ccagactgtc cggaacgccc
      541 agatcgccaa tctggcgctg tgccagcaaa cggttgctga gctggatcaa tcgcacgtag
      601 tcgtggaatg gaccgttgta atgctcgtca tggtggtgat agcagcaggc caccacttct
      661 tccggcagac gccatttttc catcagaaag ccgccaatct ggtcatgtcc cagcgcgatt
      721 aattgccgtg cctggcccat gccgatcatg tccttctcaa tcgcgtgcag cggcttttcc
      781 ggatgcttgc tggcgagctt gttgagcagg cagaactcgg gcggaaacag atgccccacc
      841 agcagcacac caatattgtg cagcaggccc gccagatagg cggtggccgg attgatcagg
      901 tccggccgat cgattttgcg cgccagactc tgtgacacca ctgcgcaata cagtgcgtgt
      961 ttccagaatg ctttcaagcc aagcggacct tcctgcggca ccttgaatgc ccggcccgat
     1021 gcgatgccca gtgcgatatt gctcaccagg tcaaaaccca gcacgcgggt aattgcatcc
     1081 tgcacggaat tgacgcggcc acgataccca taataaggcg acgcggcgta acggacgatc
     1141 tgggccgcca tgctgggatc cagttcgacg gcttccgcca gatccaccgc ggtagtcttg
     1201 gtatctgcta ccagttgaat gatcttgagg gccatggcgg gcatcgccgg caaccggtgc
     1261 atgttttcca gatactgatg gatgcggtct gcatcccctg ccgcagcgga aggaatcaat
     1321 tcttccgcaa gcgtctgcat gggattggaa ataacggcct tgggataggc gctgaacaga
     1381 aactggaagg tgtcggcatc gacgctggca agacaggaat gggtgccggc acggaaaaac
     1441 acgcgcccga cgtccatcag cgaccgatcg acgatgcagg gcagatcgta gggctctgga
     1501 atcggtggtt ccgcacccgg ttccacatcc aggaagcgct gtgatacggc atagtcgtcc
     1561 agccaggtaa attcacggcg cagcagggct tcgacgcgat tcatgtccaa cccgtgggac
     1621 aacggcaata ctgccatcag gtatgccttg cgtgcgctct gcagcaatac cggcaccgcc
     1681 acatgggccg gatcgacacc caactcaaaa cacagtttcg acaggctgct ctcgcgcacg
     1741 tggggatggg tcttgtaaac gatgcctttg cgttcgaaat accgctgaag tgaaactgct
     1801 atggccatga tcaactcgcg cccgggaaac cggttcaacc ctgatgctac cagtttagtc
     1861 gctggcgtcg gcgcacggcg aagtttcggc ggcacagtgt ccgcctttca gagaggatca
     1921 acgggtgggt gcgaactgca tcacacaccg gttttacatt gcctgatcgg gtttggtttt
     1981 ttaaaacgga atcgcagcgc agcacccagt cgctataagc attcgatgtt agccatcagg
     2041 gcatgcgcga ggaaatctgc aggctcacgt cctgacttcc gcccgccagc ttcaccacct
     2101 gggtgggacc aatccagtcg ccgctctggg gaatggcgtt gccggtggaa gaaatacggg
     2161 cctgcacgat cacatcggga aatttcgaca gggtagccat gggcgtcatg gcgttggcat
     2221 cgctcaactg caccaccaca ggccagttac ccaccggctg tttaacgacg gccagtggca
     2281 tcggcgggcc attcacggcc ttggcataaa caaacagcgt atcgctggca ctcagttgcg
     2341 aacgcgcctc tgccgccaga tccactgtca cattgagcag cggtttgccc gcggccgcaa
     2401 ccggttggct gctgtcagat acaggtgtcg agccggcctg cgccagctgc ttgcgcgcca
     2461 cctcgacgct gcgctgcaac agttgcaccg cgcgggaatc cgcattttcc cgctgaatgt
     2521 attgctgcca gatgtcgatg gcgccgttga aatcgccgcc ctggaatgct gccataccca
     2581 atgtcatgta aggttttgga ttatccggat gctgctcgat gatgcggatc agggtctgcc
     2641 gcatatccgg tgccagcacg ccgttgttct gggcgatcgc aacctgcgtg tatcccacca
     2701 cataatccac gttgtccggc tccagattga gagcgcggct gaaggccagc tccgcctgct
     2761 cggccatgcg catttgcata tagctgacac caagcagata ccagctgcgg gcatcgtgcg
     2821 gctttttctg caggtgcgcc tgcatgccgc gaatcagatc cggcagttgt acaccctgct
     2881 gttccagcgc agcgaaatcc cccgccagca tttgcggaat cattgcctcc aggcgcggtt
     2941 gcgccgccag ccagtcaccg gtttccttgc gatagccttc tgtccagtaa aggaagaatg
     3001 ccgccagcgg caccagtgca aacagcggcc ataccagcca ttttcctgcc agcgaacgcg
     3061 ggccctgatc ctgctggctg aggttcacat cgtccagcag cgtcaattcc agttcggcct
     3121 tgagctgggc gaattcgtcc gcctgcaccc ggccttcccg caaatccgtc tccagttcct
     3181 gcaaccggtc gcggtaaacc agagagttca ccgcaacgcc ggacaattcc tcccgcgccc
     3241 ggtgtttcag tagcggcaac accaccatca gcgcacccgc caaagccatc agggtcatca
     3301 ggacccacaa ctgcgtcatt tcgattcctc ttgcttggcg ccggtttccg actgttcgag
     3361 ttgccgtaac aatgcctgca cccgggcttc ctgttcaggc gtgacctgca cgggtgccgg
     3421 ttgctggcgg aatttgcgcg ccaggatgat cagtgccgcc agcagcgcca gcgcaaacgg
     3481 tccgaaccac aaaaaccagg tcagaggacg caccggcgga cggtaggtaa tgaagtcacc
     3541 gaaacgctcc accatgtgat cggtaatctc ggcattggat ttgccttcat tgatcagtcg
     3601 aaacacccgg tccttgatgt ccttggacag accggcgttg gagtccgcaa cgttctggtt
     3661 ctggcatttg gggcaacgca gttcttccac cagcacgcga aaacgctgct catcttccgg
     3721 cgttctgaat tcataggcat cgatggtggc cagcgccaaa ccacccagca tcatcaacca
     3781 gagcgcagcc accagcaaaa gcaatcgttt catgttcatg cgccaccctt ctggctatca
     3841 tccgcagcgg cgggaccagg atgtgtgccg cccatttcct gccatttcgg ccacagcagc
     3901 gtgcgccaca catcctcggt aattacgccg acgtggcggt aacggatctt gccagccgca
     3961 tcgatcaggt aggattccgg cgcgccatac acgcccaggt ccagaccgaa atcccctttc
     4021 acatcggcga tattcagttc aaacggattg ccattgatct ccaggtaccg acgggccgcc
     4081 tgcggttcgt ccttgtagtt cagccccacc atgcggatgc cttcccgcga caacgccacc
     4141 agcaccgggt gctccacgat gcaggacggg caccaggtgg cccacacgtt cagcagcaag
     4201 ggacccttga tctcgcccgc cgtgaccgtg cgtgacgggt cctccagcgt gggcagctga
     4261 aatgccggca gcggctggtc aatgcgggtg gacggcagga tcgagggatc ggagccgatt
     4321 gcgcgcccga agaaatacac cagcaaggca aagccgatca gcggcaacga aaacagcagc
     4381 ccctttctca gggacagagc gctcacggac atcaggcttt tcctcccgcc agggacgaaa
     4441 ccgcgccggc aacacggctt ttgatctgca agcggtagcg gcgatcactc agggcaatga
     4501 atccacccag cgccatgatc acgcctccgc cccacaccca gcgcatataa ggcttgtagt
     4561 aaacgcgcac cgcccagctg ccgtcctgca gcggctcgcc cagagcgaca tacagatcgc
     4621 gggtgaatcc aggatcaatg gccgcttccg tcatcacgtc acgacgcaca aaatagcggc
     4681 gtttttccgg acgcagggtg gcaatctgct cgtcgccatg caacaccttc accacgcctt
     4741 cggtggactg gaaattgggc ccccggacgt cgcgcacgcc gtcaaacaca aatacgtagg
     4801 ggccaatatt gacccggtct cccgccgcca tgcgcacatc gcgctccaca tcgaacgtcg
     4861 aggtcattgt gatgcccgcc acggtcactg cgatacccag gtgcgcgatc accatgcccc
     4921 agtaactgag ggaaaggcgg cgcaccccgg ccatgaatcc ggctttcgcc gtacggattt
     4981 tccgcagcag ttccgacagc gtgatccacc acacccacac gcataccgac aatgccagaa
     5041 acgcaatcca gtcccagccc gcatcaaaca gccagagcgc aagcgcaccc aacaacagtg
     5101 caagcaccag cgcctgcagc agcgtgcgct tgagcagagc catctgatgc cgcttccagt
     5161 tcaccagcgg cccgatgccc gtgaacacca gcagcaacag cgaaaccggt acgaacaccg
     5221 tgttgaaata aggcgggcca atggaaatct tgccccagcc cagcacgtcg aacaggatcg
     5281 gatacagggt tcccaccagc accagccccg tcgcacagat cagaatgacg ttgttgaaca
     5341 gcaaaaacat ttcgcgggaa tacagatcga aacggctctc gcttttcagc gcctgcgccc
     5401 gcaacaggaa cagcaggagg gagccgccca ccatcaatac caggaagacc agcacgaaat
     5461 agccgcgttc cggatcggca gcaaacgcat gcaccgatgt cagtacgccg gagcgcacca
     5521 ggaaggttcc cagcaaactg agggaaaaag ccagaattgc cagcaacacc gtccaggcct
     5581 tgaacacgcc acgtttttcc gtaactgcca gggaatgcat caacgccgtg cccgccagcc
     5641 agggcatgaa ggaggcattt tccaccggat cccagaacca ccagcc
//
LOCUS       DASUEG010000850         7275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1403262, whole genome shotgun sequence.
ACCESSION   DASUEG010000850 DASUEG010000000
VERSION     DASUEG010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403262"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /gene="rsxC"
                     /locus_tag="VFX11_16010"
     CDS             complement(<1..1197)
                     /gene="rsxC"
                     /locus_tag="VFX11_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX4940184.1"
                     /translation="MFNLLSPVRGGVHPAGHKDRSSALTIATAPLPERLYLPLRQHAG
                     ADALPVVRVGDRVLKGQLIAAAASEVSAPVHASSSGRVVAIGPVPAPHPSGLTANGIV
                     IECDGDDRWIDVDLPADPYSQEPMMLADRVAQAGIVGLGGAIFPAAVKLKQGARHEIK
                     TVLLNGSECEPYLTTDDRLMRERAEQIVDGARLIQHILRAYRIVIAIEDNKPEAIAAM
                     RAASEPYGAIEVEVVPALYPMGSAKQLIQEITGREVPAEGRSTSVGVLVHNVGTVFAI
                     QQALRFGRPLISRVVTVAGGCVARPRNVETRVGVPVQNLLDFCGGLTEAPAQLLMGGP
                     MMGMVLPNAQAPIIKGATGVLALSRHEVPRADASPCIRCARCVDACPMGLMPLEMAAH
                     ARADSFE"
     gene            complement(1211..1735)
                     /locus_tag="VFX11_16015"
     CDS             complement(1211..1735)
                     /locus_tag="VFX11_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEX4940185.1"
                     /translation="MLIATLVISGLGIGLGLMLGVAARAFAVEENPLTKQVEQLMPGS
                     QCGQCGFPGCSAAASALVDGSAPVTCCPPGGRALAEKLASLLGVTLDAAGAMAMPMVA
                     AIEEATCTGCTRCYRACPTDAIVGANGQIHVVLQAACTGCGNCAEACPEDCVKLQPES
                     VTLNNWHWTKPQAA"
     gene            complement(1790..2371)
                     /gene="rsxA"
                     /locus_tag="VFX11_16020"
     CDS             complement(1790..2371)
                     /gene="rsxA"
                     /locus_tag="VFX11_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX4940186.1"
                     /translation="MDYLMLLIGTVLVNNVVLVHFLGLCPFMGVSTKLGTAVGMGAAT
                     TFVMTLGAVSSWLLEHFVLQPLGIGFLRILAFILVIAGVVQFTEAVIRKVSPPLYRAL
                     GIYLPLITTNCAVLGVPLISVRAGQNLVHSALYGFGSALGFGLILIIFAGMRERLALA
                     QVPKVFVGAPVAFVTAGLLALTFMGFAGLITNG"
     gene            2796..4421
                     /gene="nifL"
                     /locus_tag="VFX11_16025"
     CDS             2796..4421
                     /gene="nifL"
                     /locus_tag="VFX11_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HEX4940187.1"
                     /translation="MTKAKRTESMIESETRAPQEPVAESAPIGVALSKEPVIIGSATL
                     TPEIFLQAVEHAPVAISITDLKANILYANRAFSTVTGYGCDEVVGKNESILSNGTTPR
                     LVYQALWSRLQQKKPWSGVLVNRRKDNSLYLAELTVAPVLSETGETLYYLGMHRDSSD
                     LHELEQRVNNQKQMIEAVVNSAPVAMVLLDQEDRIALVNPSFRTLATELAPGRPMDEA
                     VVTLQEALGEPFTALRDKGTKISSLEISFDMGGYSQRWYACHGISILLEDERAGNFFS
                     QPEARYLLLSINDITELRQRQQDSQLNALKALMAEEELLEGMRETFNGAIHRLQGPVN
                     LIGAALKMLQRRLGEQAKNDPVLLAMQEAQKAGLEALDSLTASIPIVPEEAKLPVNMN
                     QLIREVISLSIDKLLAQGIVVEWKPAMHLPWVMGRESRLRSILKQLVDNAIEAMSARN
                     ISQRDLLISTRVEKNVVRIEISDSGPGIPAALTYKVFEPFFSTKPPHKGCRGMGLPMV
                     QEIVAEHAGTVYIDTQYRSGCRMIVELPFSSAA"
     gene            4455..6035
                     /gene="nifA"
                     /locus_tag="VFX11_16030"
     CDS             4455..6035
                     /gene="nifA"
                     /locus_tag="VFX11_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HEX4940188.1"
                     /translation="MSTPMHLRTTPKPVSRTELLERHLSSLYQVARILSRTQPIEETL
                     SQVLAVLHNDAGMQHGLVTISDSEHSSLQIGAIHSDNENITRSSGSVRYRIGEGIIGG
                     ILKHGSTIVLGRISSEPRFLDRLALYDLELPFVGVPIKNAEGTTVGVLAAQPDAPADD
                     LLAERTRFMEAIAHLLAQTVRLAVNLERGEQLTTERDELRREVRAKFGFENMVVGHTP
                     AMRRVFDQIRRVAKWDSTVLILGESGTGKELIASAIHYNSPRAHRPFVRLNCAALPET
                     LLESELFGHEKGAFTGAIKQRKGRFEQADSGTLFLDEIGEISPQFQAKLLRILQEGEF
                     ERVGGSQTIKVNLRIVAATNRDLEQEVDKGKFREDLYYRLNVMAIRVPPLRERAADIP
                     ELAEFLLDKVAQRQERPLHLTDSATRLLMSYSWPGNVRELENCLERAAIMSEDGTVDG
                     DIISLTGLDEDSRPLLTHSSAPAVDFTDETMDERERVIAALEQAGWVQAKAARLLGMT
                     PRQIAYRVQTLNINMRKI"
     gene            6238..>7275
                     /gene="nifB"
                     /locus_tag="VFX11_16035"
     CDS             6238..>7275
                     /gene="nifB"
                     /locus_tag="VFX11_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HEX4940189.1"
                     /translation="MELSVLGQESGGGCSSGSCGSSSEQISHLPDHILQKVQNHPCYS
                     EDAHHYFARMHVAVAPACNIQCHYCNRKYDCSNESRPGVVSEVLTPEQAVKKVRAVAA
                     TIPQMTVLGIAGPGDPLANPDRTFSTFRMLSEQTPDIKLCVSTNGLALPASVDELAKH
                     NIDHVTITINCVDPEIGAKIYPWIYWNNKRIRGKKGAKILIEQQQKGLEMLTARGILV
                     KVNSVLIPGVNDEHLKEVSRIVKAKGAFLHNVMPLIAEASHGTFYGLMGQRSPDAAEL
                     KALQDACAGDMNMMRHCRQCRADAVGLLGEDRGDEFTMDKIDAMEIDYEAAMKVRAEL
                     RAKVTAELEAKR"
BASE COUNT         1613 a         2340 c         2058 g         1264 t
ORIGIN      
        1 ctcaaaactg tcggcacggg cgtgggccgc catttccagc ggcatcaacc ccatcggaca
       61 ggcatcaaca caacgggcgc agcggatgca gggtgacgcg tcggcgcggg gcacttcatg
      121 gcggctcagc gccagtacac ccgtggcacc cttgatgatg ggcgcctgtg cattcggcaa
      181 caccatgccc atcatgggcc cgcccatcag caattgcgcc ggcgcttcgg tgaggcctcc
      241 acagaaatcc agcagattct gcaccggcac acccacccgc gtttccacat tgcgcggccg
      301 cgccacacag ccaccggcca cggtcaccac acgggaaatc agtggccttc cgaaacgcag
      361 cgcctgctgg atcgcaaaca cggtgcccac gttatgcacc agcacgccca ccgatgtgct
      421 gcggccttcc gccggcactt cacgtccggt gatttcctgt atcagctgtt tggccgatcc
      481 catcgggtac agcgcgggca ccacttccac ttcaatcgcg ccgtagggtt cgctggcggc
      541 gcgcatggcg gcgatcgctt cgggcttgtt gtcctcgatg gcaatcacga tgcggtatgc
      601 gcgcagaatg tgctggatca gacgcgcgcc gtcgacgatc tgctcggccc gctcgcgcat
      661 caggcgatca tcggtggtga gatagggttc gcattcgctg ccgttgagca acacggtttt
      721 gatttcgtgc cgcgcaccct gtttgagttt taccgccgcc ggaaaaatgg caccgcccag
      781 accgacgatt cccgcctgcg ccacgcggtc agccagcatc atcggttctt gcgaatatgg
      841 atcggcggga agatcgacat cgatccagcg atcatcgcca tcacattcga tcacaatacc
      901 gttggcggtc aggccggacg gatgcggcgc gggcaccggc ccgatggcca ccacgcgacc
      961 ggaactggac gcatgcaccg gcgccgacac ttccgatgca gccgctgcga tcagctggcc
     1021 tttcagcacc ctgtcgccca cccgcaccac cggcagcgca tcggcgcccg cgtgctggcg
     1081 cagcggcaaa tacaaacgtt cgggcaacgg cgcagtggcg atggtgagcg ccgaggagcg
     1141 atccttgtga ccggcaggat gcacgccacc gcgcaccggc gacaacagat tgaacatgat
     1201 cgtgactcct tcaggccgcc tgcggcttgg tccagtgcca gttattcagc gtgacggatt
     1261 ccggctgcag cttcacacaa tcttcggggc aggcttcagc gcaatttccg cagccagtgc
     1321 aagctgcctg cagcaccaca tgaatctggc cattggcacc gacgatggca tccgtggggc
     1381 aggcgcgata acagcgcgtg caaccggtgc aggtggcttc ttcaatcgcg gccaccatcg
     1441 gcatcgccat ggcgccggct gcatccagcg ttacgcccag caatgacgcc agtttttctg
     1501 ccaacgcacg gccaccgggc gggcagcagg tcaccggcgc cgaaccgtcc accagagcac
     1561 tggccgctgc gctgcaaccg ggaaatccac actggccaca ctggctgccg ggcatcagct
     1621 gctctacctg tttggttagc ggattttctt ccaccgcaaa tgcgcgtgct gccacaccca
     1681 gcatcaatcc cagaccaatg cccagtcccg aaatcaccaa ggttgcgatc aacatgacat
     1741 gcctcccgag ttccgccctt tgtttgtccc accatcaccc gcttgccgct cagccattcg
     1801 tgatcaggcc agcgaatccc ataaaggtca gcgccagcag ccccgccgtc acaaacgcta
     1861 ccggcgcacc gacaaacacc ttgggcacct gcgccagtgc aaggcgttca cgcattccgg
     1921 caaaaataat cagaatcaat ccgaaaccca atgcggaacc aaatccgtac agtgcgctgt
     1981 gcaccagatt ctgtccggcg cgcacactga tcagtggcac acccagcacg gcgcaattgg
     2041 tggtaatcaa tggaaggtaa atgcccagcg cgcgatacag tggcgggctg actttgcgaa
     2101 tcacggcttc ggtgaattgc accacgccag cgatcaccag aatgaatgcc agaatacgca
     2161 gaaagccgat acccagcggc tgcaggacga agtgctccag caaccagctg ctgactgcac
     2221 ccagcgtcat gacaaacgtg gtagcagcgc ccatgcccac cgccgtgccc agtttggtgg
     2281 acacgcccat aaatggacac agtcccagga aatgcaccaa aaccacattg ttgacgagca
     2341 cagtaccgat caacagcatc agataatcca tggtgctttc aacctcgagt cacaccgtgc
     2401 gaagcgacgg catttactga catgaattcg tgtgtccacc cgggagggaa ttagcatcaa
     2461 gcatgccacc ggtttggcgg tccatcgcgg tggtctgcga tttgattgca gacttgatcc
     2521 agctcaatgt tcttcccctg aatcggtcga taccagatca ttttggtcgg atattgtttg
     2581 tcgcgtttcc aacaaaccat gcgctccgcc aggggctgct gtgccctttg caacagatcg
     2641 catcattcag gcaaagcgcg tttgtcgcat tccttccccg ggaaaaacac cgtaggtact
     2701 ttccgcacca gttcgcggca tgcaatttgc taaatccgat cagaagcacg tggcagtagt
     2761 tgcaccagaa tcaagacagc caggagttcc gtccgatgac gaaggccaag cgcaccgaat
     2821 ccatgatcga aagtgaaacc cgcgcgccgc aggagcccgt cgccgaatcc gctcccatcg
     2881 gtgtggccct ttccaaggaa ccggtcatca tcggttcggc aaccctgacg ccagaaatat
     2941 tcctgcaggc agtcgaacat gcgccggtgg ccatctccat caccgatctg aaggccaaca
     3001 ttctttatgc caatcgcgcc ttcagcactg tcaccggtta cggctgcgat gaagtagtcg
     3061 gcaaaaacga atctattctg tcgaacggca ccacaccgag actggtgtat caggccctgt
     3121 ggagtcgcct gcagcaaaaa aagccctggt ccggcgtgct ggtgaaccgg cgcaaggaca
     3181 attcgctgta cctggcggaa ctcaccgtgg caccggtgct gagtgaaaca ggtgaaacgc
     3241 tgtattatct gggcatgcac cgcgattcca gtgatctgca tgaactggaa caacgggtga
     3301 acaatcagaa acaaatgatc gaagcggtgg tgaattccgc cccagtggcg atggtgctac
     3361 tggaccagga agaccgtatt gccctggtga atccgagttt ccgcacgctg gcgacggaac
     3421 tggcgccggg ccggccgatg gatgaagcgg tggtcacctt gcaagaagcg ctgggcgaac
     3481 cttttacggc actgcgcgac aagggcacca aaatttccag cctggaaatt tccttcgaca
     3541 tgggcggcta ttcccagcgc tggtatgcct gccatggcat ttccatcctg ctggaagacg
     3601 aacgcgcggg caatttcttc tcccagccgg aagcccgcta cctgctgctg tccatcaatg
     3661 acatcaccga actgcgccag cgccagcagg attcccagct gaacgcgctc aaggctctga
     3721 tggcggaaga ggaattactg gaaggcatgc gtgaaacctt caacggcgcc attcaccgct
     3781 tgcaggggcc ggtaaaccta atcggcgcgg cactgaaaat gctgcagcgg cggttgggcg
     3841 agcaggcaaa aaacgatccg gtattgctgg cgatgcagga agcgcaaaag gccggtctgg
     3901 aagcactgga cagtctcacc gcctcgattc cgattgttcc cgaggaagcc aaactgccgg
     3961 tgaacatgaa ccagctgatt cgcgaagtga tcagcctgtc catcgacaag ctgctggcgc
     4021 agggcatcgt ggtggaatgg aaacccgcga tgcacctgcc ctgggtgatg ggccgcgaga
     4081 gccggttgcg cagcatactg aaacagctgg tggacaatgc gatcgaagcc atgagcgcgc
     4141 gcaacatcag ccagcgcgat ctgctgatca gcacccgtgt ggaaaaaaat gtcgtgcgca
     4201 tcgagatcag cgattcgggg cccggcattc ccgccgcgct tacctacaag gtgttcgagc
     4261 ccttcttttc caccaagcca ccacacaagg gttgccgcgg catgggcctg ccaatggtgc
     4321 aggaaatcgt ggcggaacac gctggcaccg tctacatcga cacccagtac cgcagcggtt
     4381 gccgcatgat cgtggaactg cccttttcct ccgccgcctg atcgcgaccg cattccggga
     4441 gagaagtgat tgccatgagt acgccgatgc atctgcgaac cacacccaag ccggtcagcc
     4501 gcaccgagct gctggaacgt catctgagtt cgctgtacca ggtggcccgc atactgagcc
     4561 gcacccagcc catcgaggaa accctgtcgc aggtgctcgc ggttttgcac aatgatgccg
     4621 gcatgcagca cggcctggtg accatttccg attccgaaca cagcagcctg cagattggcg
     4681 ccatccacag cgacaatgaa aacatcaccc gcagctccgg ctcggtgcgc taccgcattg
     4741 gtgaaggcat tatcggcggc attctgaagc acggctctac catcgtactg ggccggattt
     4801 catcggaacc acgttttctg gatcgcctgg cgctgtatga tctggaactg ccttttgtcg
     4861 gcgttcccat caagaacgcg gaaggcacca ccgtaggcgt tctggcagcg caaccggatg
     4921 cgccagccga tgacctgttg gcggaacgca cccgtttcat ggaagccatc gcccacctgc
     4981 tggcgcaaac cgtgcgtctg gcggtgaatc tggaacgcgg cgaacagctc accaccgagc
     5041 gcgacgagct gcgccgcgaa gtgcgcgcaa aattcggctt tgaaaacatg gtggtgggcc
     5101 acacaccggc catgcgccgg gtattcgacc agatccgtcg cgtcgccaag tgggacagca
     5161 ccgtgctgat cctcggcgaa tccggcaccg gcaaggagct gatcgccagc gccatccact
     5221 acaactcgcc gcgcgcacac cgaccgttcg tgcgcctgaa ctgcgccgca ctgccggaaa
     5281 ccctgctgga atcggaattg ttcggccacg aaaaaggcgc gttcaccggt gccatcaaac
     5341 agcgcaaggg acgtttcgag caagccgaca gcggcacact tttcctcgat gaaatcggtg
     5401 aaatctcgcc gcagttccag gccaagctgc tgcgcatact gcaggaaggc gaattcgagc
     5461 gcgtgggcgg cagccagacc atcaaggtga acctgcgcat cgtcgccgcc accaaccgcg
     5521 atctggaaca ggaagtggac aaaggcaaat tccgcgagga cctgtattac cggctcaatg
     5581 tcatggcgat ccgcgtgccg ccgctgcgtg aacgcgccgc cgacattccc gagctggcgg
     5641 aattcctgct ggacaaagtg gcgcaacggc aggaacgtcc actgcacctg acggacagcg
     5701 cgacccgcct gctgatgagt tacagctggc ccggcaacgt gcgcgaactg gaaaactgcc
     5761 tggaacgcgc cgccatcatg agcgaagacg gtaccgtcga cggcgacatc atttccctca
     5821 ccggactgga cgaagattcc cgcccgctgc tgactcattc ttccgccccc gccgtggatt
     5881 tcaccgacga aaccatggac gagcgcgagc gcgtcatcgc tgcgctggaa caggccggct
     5941 gggtgcaggc caaagccgcg cgcctgctgg gcatgactcc gcgccagatc gcctaccgcg
     6001 tgcaaaccct caacatcaac atgcgcaaaa tctgacagcg cacagcggcg gcactgtgtc
     6061 gtacacccca cacaacgccg ctggcgtttt tgtctggagc cccacaaaaa ctccgcattt
     6121 tccagcctta aactctttaa aaacaagact ttaatttcgc ggcatatcaa ttgctatcca
     6181 tacggtggaa gccagactca ccacctcgcc cgcaagggca ccgaccggag accgaccatg
     6241 gaactgagcg tattggggca ggaaagcggc ggcggctgct catcaggcag ctgtggcagc
     6301 agcagcgaac agatttccca cctgccggac cacattctgc aaaaggtaca gaaccacccc
     6361 tgctattccg aagacgcgca ccactatttc gcccgcatgc acgtggcagt agcgcccgcg
     6421 tgcaacatcc agtgccacta ctgcaatcgc aaatatgatt gctcgaacga atcgcgcccc
     6481 ggcgtcgtat cggaagtgct gacaccggaa caggccgtga aaaaagtccg ggccgtagcc
     6541 gccaccattc cgcagatgac cgtgctgggc attgcagggc cgggcgatcc actggcgaat
     6601 ccggatcgca ccttcagcac cttccgcatg ctgagcgaac aaactccgga catcaaactg
     6661 tgcgtctcca ccaacggcct ggcattgccg gcgtcagtgg acgagctggc caaacacaac
     6721 atcgaccacg tcaccatcac catcaactgt gtcgatcccg aaatcggcgc caaaatctac
     6781 ccttggatct actggaacaa caagcgcatt cgcggcaaga agggcgcgaa gatcctgatc
     6841 gagcagcagc agaagggcct ggagatgctg accgcgcgcg gcatcctggt gaaggtcaac
     6901 tcggtgctga tccccggggt caacgacgag catctcaagg aggtcagccg catcgtcaag
     6961 gccaagggcg ccttcctgca caacgtcatg ccgctgatcg cggaagcgag ccacggcacc
     7021 ttctacggcc tgatgggcca gcgcagcccg gacgccgcgg aactcaaggc actgcaggac
     7081 gcctgcgccg gcgacatgaa catgatgcgc cactgccgcc agtgccgcgc cgacgcggtc
     7141 ggcctgctcg gcgaggaccg cggcgacgaa ttcaccatgg acaagatcga cgccatggag
     7201 atcgactacg aagccgccat gaaagtgcgt gcagaactgc gcgccaaggt gacggccgag
     7261 ctggaggcca agcgt
//
LOCUS       DASUEG010000851         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1844878, whole genome shotgun sequence.
ACCESSION   DASUEG010000851 DASUEG010000000
VERSION     DASUEG010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844878"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFX11_16040"
     CDS             complement(<1..1352)
                     /locus_tag="VFX11_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4940190.1"
                     /translation="MADIIAASHGLAVTQAKDISQSSLALEADVVVIGSGAGGAVAAY
                     ELARAGKSVIVLEAGPYVPSSEFTEDFSQAIVQLYEDKGTQTNADGDLMVLQGKCVGG
                     STVVNGCVAFRTPDYILDDWQKQYGLTNLTVDALEPYFARVEANLGIHVNPEHEINRN
                     SAVLREGAKKLGISWKPFQRNIRECALTGHCLSGCATDRKQSMLVTYLPWATAHGARI
                     YADTKVTQIRAENGKATGVVAEVTDPKTGVKKADVSVKAKLVVLAAGAVQTPLLLQRS
                     NLANSSGQVGKNFACHPSTFVVGQYEEDIHPWRGAMLGIYVDEWAHPSKGGYILEGGG
                     AGAVELSAVTQPGLGEDFMDYMANLKKLAGIVTLIHDHNVGEISWDDDKKKIDYRVAE
                     ADYPAMKAAIASAVKLHFASGAKKVYLPTVAHAVVENPADADAKVAAIELGLQSLRMV
                     S"
     gene            complement(1441..2049)
                     /locus_tag="VFX11_16045"
     CDS             complement(1441..2049)
                     /locus_tag="VFX11_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940191.1"
                     /translation="MTQNKNKAASDVAAALAPLSRRSVLKTGLWAVAGVSALAAGGFA
                     LLRPSPRDSLPVPAGLVHLEAQHYHLFSRLIEILLPTEGTTLLPPAQVPVVNHIDSLL
                     DALDPAIRKQLLTGLALFDNAAVVTHFKRFVDLPDAEALAYVGGWVNSGVMPRRALGF
                     VVTKLTHTGYWMDSQTWPMVEFDGPVSRRWGIPSRGNQPLPG"
     gene            complement(2198..3052)
                     /locus_tag="VFX11_16050"
     CDS             complement(2198..3052)
                     /locus_tag="VFX11_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940192.1"
                     /translation="MTSLTRKKCFITGAASGIGRATAIAAAAQGAELVLTDIDAAGLE
                     AVAAQIRQSGGKVLHSRALDIADYAAVRQYADDLHAQFGSLDVLMNIAGIAIWGTVET
                     LAHAQWRRCIEVNLMGPIHVMECFLPPMIKAGRGGCLVNVASAAGLFPLPMHAPYSAS
                     KFGLRGVSEVLRQDLRRHGIHVCLVCPGAVNTGLVQTVQIAGIDLQHPQAVKLRQDFQ
                     RHAVTPEQAARAILDGIAARRWMVFTSPDIRFGYWMQRKWAWPFDLAMRWLNNRLHAV
                     VQAAQTRG"
BASE COUNT          548 a         1013 c          986 g          586 t
ORIGIN      
        1 gacaccatgc gcaacgactg caggcccagc tcgatcgccg ccactttggc gtcggcgtca
       61 gccggatttt ccactacggc atgcgccacg gtgggcaggt aaaccttttt cgcgccgctg
      121 gcaaaatgca gtttcaccgc cgacgcaatc gccgctttca tcgccgggta atccgcttcc
      181 gccacccggt aatcgatctt ctttttgtcg tcatcccacg aaatttcacc cacgttgtga
      241 tcgtggatca gggtgacgat gcccgccagc ttcttgaggt tggccatgta atccatgaag
      301 tcctcgccca gacccggctg ggtgacggca gacagctcca ccgcaccggc gccgccacct
      361 tccagaatgt agcctccctt cgacggatgc gcccactcgt ccacataaat gcccagcatg
      421 gcgccgcgcc agggatgaat gtcttcctcg tactgtccca ccacaaaggt ggacggatgg
      481 caggcgaaat tcttgcccac ctggccggac gaattcgcca gattggaacg ctgcagcaac
      541 agcggggttt gcaccgcgcc cgccgccagc accaccagtt tcgccttcac gctgacatcg
      601 gcttttttca cgccggtttt aggatcggtc acttccgcca ccacgccggt ggccttgccg
      661 ttttccgccc ggatctgcgt gactttggtg tcggcataga tgcgggcgcc gtgggcggtg
      721 gcccagggca gataggtcac cagcatcgac tgcttgcgat cggtggcgca gccggacaga
      781 cagtggccgg taagcgcgca ttcccggatg ttgcgctgga acggcttcca ggaaatgccg
      841 agttttttcg cgccttcgcg cagcacggcc gagttgcgat tgatttcgtg ttcgggattc
      901 acgtggatgc cgaggttggc ttccacccgg gcgaaatacg gctccagtgc gtccacggtc
      961 aggttggtga ggccgtactg tttttgccag tcatccagga tgtagtccgg cgtgcggaac
     1021 gcgacgcaac cgttcaccac ggtggaaccg ccgacgcatt tgccctgcaa taccatcagg
     1081 tcgccgtcgg cgttggtctg ggtgcccttg tcttcataca gctgcacgat ggcctggctg
     1141 aaatcctcgg tgaattcact ggacgggacg taggggccgg cttccagcac gatgacggat
     1201 ttgcccgccc tggccagttc ataggccgcg actgcgccgc ccgcgccaga gccgatgaca
     1261 acgacgtctg cttccagcgc caggctgctc tgggaaatgt ctttcgcctg ggttactgcc
     1321 aggccgtggg atgctgcaat gatgtctgcc atgttgtgtg ctctccgccg ggggtcaggc
     1381 ccgggccggt actgtgaatg ctgtgctgta gatgccgggt tcgtaagtca gggggccggt
     1441 tcagcccggc agcggctggt tgccacggga cggaatgccc cagcgccgcg agacagggcc
     1501 atcgaactcc accatcggcc aggtctggct gtccatccag taaccggtgt gggtgagttt
     1561 ggtgaccacg aaacccagcg cgcgcctggg catgacgccg gaattcaccc agccgccgac
     1621 ataggccagg gcctcggcgt caggcaggtc cacaaagcgc ttgaagtggg taaccacggc
     1681 ggcgttgtcg aacagggcca gcccggtcag caactgcttg cggatggcgg ggtccagcgc
     1741 gtccagcagg ctgtcaatgt gattaacgac tggcacctgc gcgggcggca gcaaagtcgt
     1801 gccttcggtg ggcaacagga tttcgatcag gcggctgaac aggtggtagt gctgggcttc
     1861 cagatggacc agtcccgccg gcaccggcag gctgtcacgg ggcgacgggc gcagcagggc
     1921 aaaaccgccg gcggccaggg cacttacgcc ggcaacggcc cacaatccgg tcttcagaac
     1981 agagcggcgc gacaggggcg ccagtgcggc cgcgacatcg ctggccgcct tgtttttgtt
     2041 ttgggtcatg ggaaaacgtc cctcggcaat ctcgtggctg attggtcaca atcggcccag
     2101 tgtacgcaaa acaatctgtc tgccaagcga aatttaatca actgctttaa tccggacggg
     2161 taccggaccg gcccctgacc ccccttggcg ctggcggtca gccccgggtc tgggccgcct
     2221 gcaccaccgc atgcaggcgg ttgttgagcc agcgcatcgc cagatcgaag ggccaggccc
     2281 acttgcgctg catccagtag ccaaagcgga tatccggcga ggtgaacacc atccagcgcc
     2341 gcgccgcgat gccgtccaga atcgcgcggg ccgcctgttc cggcgtgacg gcgtggcgct
     2401 ggaaatcctg gcgcagcttg accgcctgcg gatgctgcag gtcgatgccg gcgatctgca
     2461 ctgtctgcac caggccggtg ttcactgcgc cgggacacac caggcacaca tggatgccat
     2521 gccggcgcag atcctgccgc aacacttccg acacaccgcg caaaccgaat ttgctggcac
     2581 tgtagggcgc atgcatgggc agcgggaaca gcccggccgc cgacgccaca ttcaccaggc
     2641 aaccgccgcg ccccgcctta atcatcggcg gcagaaaaca ctccatcaca tggatcggcc
     2701 ccatcaggtt gacctcgatg cagcggcgcc actgcgcgtg ggccagcgtt tccaccgtgc
     2761 cccagatggc aatgccggcg atgttcatca atacgtccag actgccgaat tgcgcgtgca
     2821 ggtcgtcggc gtactgccgc accgcggcat aatcggcaat gtcgagggcg cgggaatgca
     2881 gcaccttgcc gccgctctgg cggatctgcg ctgccacggc ttccaggccg gctgcatcga
     2941 tgtcggtcag caccagttcg gcgccctggg cggccgccgc gatggccgtg gcccggccaa
     3001 tgccgctcgc ggcgccggtg atgaagcatt tttttcgggt caggctggtc atgccgggtt
     3061 cctttgccac tggttcaacg ggagtttctg catggtgcgg ccgctgcccg gtgaacgcca
     3121 gtgcgcaaac caa
//
LOCUS       DASUEG010000852         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_298406, whole genome shotgun sequence.
ACCESSION   DASUEG010000852 DASUEG010000000
VERSION     DASUEG010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298406"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1536
                     /locus_tag="VFX11_16055"
     CDS             226..1536
                     /locus_tag="VFX11_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4940193.1"
                     /translation="MLDDLIPLLIYLLPLLLIGGVYVYLQKRRDKANLTLLQEARESG
                     LHEPASLHPVIDTTRCVGCGACVNACPEHNVLGLIQGKAQLIGAANCIGHGACQKACP
                     VGAIDLVFGTATRGVDIPLIAADFQTSVPGIYIAGELGGMGLIRNAIEQGRQAMEAMV
                     KALPKRVEAPLDAVIVGAGPSGLSAALAAKAAGIRYITLDQEVTLGGTVAHFPRGKLV
                     MTQPATLPLVGKVKLTETSKEHLLAFWNDVLQRHPLNMALGEQVQHIERSGSGFRVIT
                     GSRQYQCHQVLLAIGRRGTPRKLNVPGEDASKVVYRLIDPEQYRGQAVLVVGGGDSAL
                     EAAISIADEQDTQVTLAYRGDSFSRAKAKNRERVQALVQAKKIDVFLNSSPATIEDKV
                     VILQTPEGVKVIANDSVIICAGGELPTGFLKKIGINVETKYGTA"
     gene            1523..>2863
                     /locus_tag="VFX11_16060"
     CDS             1523..>2863
                     /locus_tag="VFX11_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4940194.1"
                     /translation="MALPERARWIWLPFLLVMFLLPAFASDVWQQYATHIRLRQYQQA
                     AQLIEPLARSRDPKACYELAQLYRNGTGVKKDVTKARQLLETAAQQGHTDSQYLLGVF
                     YSKGIGGAKDPQQAQRYLQQAAAQKHDKAEKALEQLGAVANDSSLDAQQVTNAALKGD
                     LATLRTAIATRQFLDATDSDGNHLLALAASRGQQPAAELLKQQGMNINQQNHNGETAL
                     HLAAAAQDQPMVRWLLDNGAKPDIRNKLGRTPLHIAVEKNDPTMVESMLSHHADPQLA
                     DNAGNTAVQLAQARQNAAVLLVFQRRGVTTAHDSTLQQRVAAARQTADDIAPLQLAVE
                     RGDLALVKALLNDTPDPWRPNAQGHTVITLAAQQKQSSILAYLLQSAKGKGLMGPHGR
                     NALFFAVTADRRDNLALLLSSGADPLQQDDNKKNAVTFAVESGSALSSDLLKPVP"
BASE COUNT          644 a          877 c          836 g          506 t
ORIGIN      
        1 ggtgccgcga caatcgactc agccggcatc ggatctccaa cattcagcac attgcgacag
       61 gatctaaagc aggacaggcc gacacattcc gcccacaccc tgctttaaca gtaagccccg
      121 attcttaaat aacccttaag aaatctgtgc tattttttgt tctgccaaaa gcaacacagc
      181 tccgcctgca tttttgcgaa cctcgtcaca cacttaggct catttatgct ggatgacctt
      241 atcccgctgc tgatctacct gctgcccctg ctgctgatcg gcggtgttta cgtctatctc
      301 cagaaacggc gcgacaaagc gaacctgacg ctgctgcagg aagcgcgcga gtccggcctg
      361 catgagccgg cgtcgctgca ccctgtcatc gacactaccc gctgcgtggg ctgtggcgcc
      421 tgcgtgaatg catgccctga acacaacgtg ctgggcctta tccagggcaa ggcccaactg
      481 atcggcgcgg ccaactgcat tggccatggt gcgtgccaga aagcctgccc ggtgggtgcc
      541 atcgatctgg tcttcggcac cgcgacgcgg ggcgtggaca ttcccctcat agcggcggat
      601 tttcagacct cggtacccgg catctacata gcaggcgaac tggggggcat gggcctgatc
      661 cgcaatgcga tcgagcaggg ccgtcaggcc atggaagcca tggtgaaagc gcttcccaaa
      721 cgcgttgagg cgcccctgga tgccgtgatt gtgggcgccg ggccgtcggg gttgtccgcc
      781 gcgctggcag caaaagccgc cggcatccgc tacatcacac tggatcagga agtgacgctg
      841 ggcggcacgg tggcacattt tccccggggc aaactggtga tgacccagcc cgctacgcta
      901 ccgctggtgg gaaaagtgaa gctcactgaa accagcaagg aacatctgct ggcgttctgg
      961 aatgacgtgt tgcagcggca tcccctcaac atggcactgg gggaacaggt tcagcacatc
     1021 gagcgctctg gaagcggatt tcgcgtgatc accggcagca gacaatatca gtgtcaccag
     1081 gtgctgctcg ccatcggccg ccgcggcaca ccgcgcaaac tgaatgtgcc cggcgaagat
     1141 gccagcaagg tggtgtaccg gttgatcgat cctgaacaat atcgcggcca ggccgtgctg
     1201 gtggttggcg gcggcgacag cgcactggaa gcggccatca gtattgccga cgagcaggac
     1261 acacaggtca cgctggcgta tcgcggcgat tccttcagcc gcgccaaggc gaaaaaccgc
     1321 gagcgggtgc aggcgctggt gcaagccaaa aaaatagacg tatttcttaa cagctcacct
     1381 gccaccatcg aagacaaggt ggtgatcctg caaacgcccg agggcgtaaa ggtcatcgcc
     1441 aatgacagcg tgatcatctg cgccggcggg gaattgccaa ccggatttct caaaaaaatc
     1501 ggcatcaacg tggaaaccaa atatggcact gcctgaacgc gcgcgctgga tctggttgcc
     1561 gtttttgctg gtcatgtttt tgttgcccgc gttcgccagc gacgtgtggc agcaatatgc
     1621 cacccatatc cgtctgcgcc agtatcagca ggccgcccaa ttgatcgaac cgctcgcacg
     1681 atcccgtgat cccaaagcct gctatgaact ggcacagctg tatcgcaatg gcaccggggt
     1741 taaaaaggat gtgacgaaag cgcgacaact gctggaaacc gccgcgcagc agggacacac
     1801 cgacagccag tatctgctgg gcgtgttcta cagcaaaggc atcggtggcg ccaaagatcc
     1861 gcaacaggcg caacggtatc tgcaacaggc tgccgcgcaa aaacatgaca aggcggaaaa
     1921 agcgctggaa cagctgggag cagtcgccaa cgattccagc ctcgatgcac agcaggtgac
     1981 gaatgccgcg ctgaaaggtg atctggccac gttgcgcacc gccattgcaa cccggcaatt
     2041 tctggatgcc acggacagcg acggcaacca tctgctggca ctggcagctt cgcgcggcca
     2101 gcaaccggcg gcggaattgc tgaaacagca gggcatgaat atcaatcaac agaatcacaa
     2161 cggcgaaacg gcgttgcacc tggccgccgc ggcgcaggat caacccatgg tgcgctggct
     2221 gctggacaac ggcgccaaac ccgacattcg caacaaactg ggccgaaccc cattgcatat
     2281 tgcagtggaa aaaaatgatc cgacgatggt ggaatccatg ctgagccatc atgccgatcc
     2341 gcagcttgcc gataacgctg gcaacaccgc cgtgcaactg gcgcaggcac gtcaaaatgc
     2401 tgcggtacta ctggtatttc agcgccgtgg cgtgacaacg gcgcacgatt cgacgctgca
     2461 gcaacgggtg gcagccgcgc gccagacagc ggacgatatt gcccccttgc agctggctgt
     2521 cgagcggggc gatctggcgc tggtaaaggc cctgctcaac gacacccccg atccctggcg
     2581 gcccaacgcc cagggccaca cggtgatcac cctggccgca caacagaagc agtcatcgat
     2641 tcttgcttac ctgctgcaat cagccaaagg caaaggcctg atggggcccc acgggcgcaa
     2701 cgcattgttc tttgctgtca ccgccgatcg ccgcgacaac ctggcgctgc tgctgtccag
     2761 cggggccgat cccctgcaac aggatgacaa caagaaaaac gccgtcactt tcgccgtgga
     2821 aagtggctcg gctctctcgt ctgatttgct gaagccggtg ccg
//
LOCUS       DASUEG010000853         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1797830, whole genome shotgun sequence.
ACCESSION   DASUEG010000853 DASUEG010000000
VERSION     DASUEG010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797830"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFX11_16065"
     CDS             complement(<1..825)
                     /locus_tag="VFX11_16065"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX4940195.1"
                     /translation="MPRKLTTLACLTLVLSLTACSSGQQASTSATDSPADPLASIHAM
                     VEQAEKMESPKAEKLLIDAANQLLARGDLEEAGRILVPLDSSMLPAKIKADHVIALAR
                     LAMAQEKYARVTDLLTTDERGLISASATLDAERLNQISLLRAQAWEAQQNYLAAARER
                     IFVSPMLADTDEFNRNHQQIWADLNNVPEDSLETLSRTAAIPEIQGWLELAWIAKNQQ
                     DNIDQQLRDVRRWQVKFAGHPAARQLPDSLRVLTEMSDQKPMDVALLLPLQGKYRQS"
     gene            961..1806
                     /gene="rsmI"
                     /locus_tag="VFX11_16070"
     CDS             961..1806
                     /gene="rsmI"
                     /locus_tag="VFX11_16070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX4940196.1"
                     /translation="MASGTLYIVATPIGNLNDITLRALEVLKQVDLIAAEDTRHSKKL
                     LDAYSINTRLMSVHDHNEADRAGFLVGLLAKGQSVALISDAGTPLISDPGYRLVGAVR
                     EAGHPVVTIPGACAAVAALSIAGLPTDRFLFAGFLSAKQQARRTALEGLLRESATLVF
                     YESPKRIVELVQDIALVLGESRQIAVAKELTKAFETVQTGTPSEVLAWLEADPHHQRG
                     EFVVLVEGFAADESDAVLDPKILRALALAREQMPLKKACALVSELTGAPKNQLYDIAL
                     KQFNA"
     gene            1838..2186
                     /gene="rnpB"
                     /locus_tag="VFX11_16075"
     ncRNA           1838..2186
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX11_16075"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2514..2972
                     /gene="mraZ"
                     /locus_tag="VFX11_16080"
     CDS             2514..2972
                     /gene="mraZ"
                     /locus_tag="VFX11_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX4940197.1"
                     /translation="MFRGPNILNLDAKGRLVLPTRYRDALLEKCQGQLVATIDTEERC
                     LQLYPMPEWLEIERKINALPSFNKAARRVQRLLVGHATEVEMDAAGRIGIPPVLRSYA
                     DLDKKIMLLGQGHRFEIWSEDLWNQKRDAWLADDSEDDDGMPDQMLSLVL"
     gene            2972..>3548
                     /gene="rsmH"
                     /locus_tag="VFX11_16085"
     CDS             2972..>3548
                     /gene="rsmH"
                     /locus_tag="VFX11_16085"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX4940198.1"
                     /translation="MTGPAFSHETVLLQEAVDALLTDRQGIYIDGTFGRGGHARLALS
                     QLDSAATLLVFDKDPQAIATADALAAADSRLQPVHDSFVALGREVDARGWRGQVQGIL
                     LDLGVSSPQLDDPERGFSFMNDGPLDMRMNNQAGLSAADWLNSATADEIAEVLREYGE
                     ERFAWRIAQAIVARRVEEPLSRTRQLAAWIED"
BASE COUNT          654 a          971 c         1154 g          769 t
ORIGIN      
        1 ggattgccgg tacttgccct gcagaggcag cagcagggca acatccatcg gtttctggtc
       61 gctcatttct gtcagtacgc gcaggctgtc gggcagttgg cgtgcggcgg ggtggccagc
      121 gaatttcacc tgccagcgac gcacgtcgcg cagctgctgg tcaatattgt cctgctggtt
      181 cttcgcaatc caggccagtt ccagccagcc ctggatttcc ggaatggcgg cggtgcggct
      241 gagtgtttcc agggaatctt ccggcacatt gttcagatcg gcccagattt gctggtggtt
      301 gcggttgaat tcatcggtgt ccgccagcat gggcgataca aaaatgcgtt cgcgcgcggc
      361 ggccagataa ttctgctggg cttcccacgc ctgtgcccgc agcaggctga tctggttcag
      421 gcgttccgca tccagtgtgg cggaggcgct gatcaggccg cgctcgtcgg tggtgagcag
      481 gtcggtgacg cgggcgtatt tttcctgggc catggccagg cgggcgaggg cgatgacgtg
      541 atcggccttg atcttggccg gcagcatgga ggaatcgagc ggcaccagta tccggccggc
      601 ttcttccaga tcgccacggg ccagcagctg gttggcggcg tcgatgagga gtttttccgc
      661 cttggggctc tccatttttt ccgcctgttc caccatggca tggatgctgg ccaggggatc
      721 agcgggcgaa tccgtggcgg aggtggaagc ctgttggccg gaggagcagg ctgtcaggga
      781 cagcaccagg gtcaggcagg caagtgtggt aagcttccgc ggcatctcgg gtgtcttcat
      841 cgaattcgga ttcagttcgg gattcttgag gttcaagtaa gattcaaggt tttggttttt
      901 cagatctggc ccggtctttt cagtctagcc aggtcagggc atttcaggtc gaacgtaacc
      961 atggcgagtg gaactcttta tattgtcgca acacccatcg ggaatctcaa cgacattacg
     1021 ttgcgggcgc tggaggtgtt gaagcaggtg gacctgattg ccgctgagga cacgcgccac
     1081 agcaagaaac tgctggacgc ctacagcatc aacacccggc tgatgtcggt gcacgatcac
     1141 aacgaggccg acagggccgg gtttctggtg gggcttctgg ccaaggggca gtcggtggcg
     1201 ctgatttccg atgccggcac accgctgatc agcgatcccg gctaccgcct ggtgggggcc
     1261 gtgcgtgagg cggggcatcc ggtggtgacc attccaggtg cctgcgcggc ggtggcggca
     1321 ctgtcgattg ccggtctgcc caccgaccgc ttcctgtttg ccggtttcct gtcggcaaag
     1381 cagcaggcgc ggcggacggc actggaggga ctgctccgtg aatccgccac cctggtgttc
     1441 tacgaatccc ccaagcgtat tgtagaactg gtgcaggaca ttgcactggt gctgggagaa
     1501 agccgtcaga tcgccgtggc gaaggagctg accaaggcat ttgaaaccgt gcagaccggc
     1561 acgccatcgg aggtgctggc ctggcttgaa gcggacccgc atcatcagcg cggcgagttc
     1621 gtggtgctgg tggaagggtt tgcggcagac gaatcggacg ccgtgctcga tcccaaaatc
     1681 ctgcgcgcgc tggcgctggc ccgtgagcag atgccgctga aaaaggcctg tgcgctggtg
     1741 tcggagctga ccggtgcccc caagaaccag ctctacgata ttgccctgaa acaattcaat
     1801 gcttgaatcc ttagacaatt agctatattt tgcaccggaa gtcagccaga cagtcgctct
     1861 tcgtttcggc ggagggagga aagtccgggc tccacagggc aaggtgccag gtaacgcctg
     1921 ggcggcgtga gccgacggaa agtgcaacag aaagcaaacc gcctaagccc gcaagggccg
     1981 gtaagggtga aagggtgcgg taagagcgca ccgcatggct ggcaacagtt catggcacgg
     2041 caaaccccac ctggagcaag accaaatagg aatccattgg cgtggcccgc gctggattcg
     2101 ggtaggttgc tagaggcgtg cggcgacgta cgtcccagag gaatgactgt ccacgacaga
     2161 acccggctta caggctgact ttattttttt cccttctcta tctgcggttt aataaccgcg
     2221 ctcccctgat cgatcgatcc atattgccaa ataatcttcc aagctagaac ccgctttaag
     2281 agccctttcc atccccctga atttgcttga tttttttgag ccattttttg cctttttgtg
     2341 tgctgtttcg cgcgtaagtt attgtttttg cgcggcaaat tggcattttt ttgcccggtt
     2401 ttttcttgac gcccgatttt gcagtcccta tagtgtcacg gagtgggaag ttgtgggttt
     2461 tggtgggaaa aaagggtttc agatagcctt cgggtgggaa aaagggaagc gtggtgtttc
     2521 gaggtccgaa catactgaat ctggacgcga aaggccggtt ggtactgcca acccgctatc
     2581 gggacgcttt gctcgaaaaa tgccagggcc agctggtggc caccatcgac accgaagagc
     2641 gctgcctgca actgtacccc atgccggaat ggctcgaaat cgagcgcaag atcaacgcac
     2701 ttccctcgtt taacaaggcc gcccgccgcg tgcagcgcct gctggtgggt catgccaccg
     2761 aggtggagat ggacgccgcc ggacgcattg gcattcctcc cgtgctgcgg tcctacgccg
     2821 atctggacaa gaagatcatg ctgctgggtc agggtcaccg ttttgaaatc tggtcggaag
     2881 atctgtggaa tcagaagcgc gatgcctggc tggcggacga tagcgaggac gacgacggca
     2941 tgccggacca gatgctgtcg ctggtgcttt gatgaccggg ccggcatttt cccatgaaac
     3001 cgttttgttg caagaggcgg tggacgcatt gttgactgac aggcagggca tctacatcga
     3061 tggcactttc gggcgtggcg gacatgcccg gctggcgctg tcgcagctgg attctgctgc
     3121 caccctgctg gtgttcgaca aagaccccca ggccattgcc acggcggacg cgctcgctgc
     3181 cgccgattcc cgcctgcagc cggtgcacga ttcgtttgtg gcgctgggac gcgaagttga
     3241 tgcccgcggc tggcgcggcc aggtgcaggg cattctgctg gatctggggg tgtcgtcgcc
     3301 ccagctggac gatcccgagc gcggtttttc ctttatgaat gatggtccgc tggatatgcg
     3361 gatgaacaac caggccggcc tgtccgctgc cgactggctg aattccgcga ctgccgacga
     3421 aattgccgag gtgctgcgcg aatacggtga agagcggttt gcctggcgca tcgcgcaggc
     3481 gattgtcgcg cgccgtgtgg aagagccgct gtcgcgcacc cgtcagctgg cagcctggat
     3541 cgaggacg
//
LOCUS       DASUEG010000854         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_11587, whole genome shotgun sequence.
ACCESSION   DASUEG010000854 DASUEG010000000
VERSION     DASUEG010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11587"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..550
                     /locus_tag="VFX11_16090"
     CDS             194..550
                     /locus_tag="VFX11_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX4940199.1"
                     /translation="MKGKLLALGIFSILMTGVAQADDHCDDPVADWQPRDALRHELES
                     NGWQVKRIRVDDGCYEVDGIDSNGNRFEATFTPASLEIRELEIRFRKGGRAGDYLDKD
                     RPHHRHGAPAGTPASP"
     gene            602..1072
                     /locus_tag="VFX11_16095"
     CDS             602..1072
                     /locus_tag="VFX11_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HEX4940200.1"
                     /translation="MKTKQLFLALGTALALPALSLAKPVTLTTELSAYSGDGAYVALY
                     LTDANGQYKRTLWIAGKKPKYYKHLADWARGSGQNKAEYDGMTGASVHSGKTLSVTVE
                     VPDALIDAGNQIHIDTAVEKKRDNRSEVVVPLTTDGAGKPTAGKGYVKAFTYSF"
     gene            1091..>2474
                     /locus_tag="VFX11_16100"
     CDS             1091..>2474
                     /locus_tag="VFX11_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX4940201.1"
                     /translation="MLRQLHSLPGLVAAVLVAVLAVSGAVLSISPALERWSATLPATG
                     QMTVADLAGKVATQYPLVEQIQRTPSGSIIVYFRDGDVSGVDLFDPVAGKSLSAYESP
                     SLMRWVRNLHRSLLLDTEGRIATGTGAVLMVLLVMTGSFLLAARLGGWRNILGPVRGS
                     LSQRLHGILSRYAMLGLLLSAMTGMYLSAGSLELIPEALEAEPEFPESGELAAPLPVH
                     TLAALKAIDLNDLREMVYPYPGDPADNYSVRTRQGAGFVHASSGELLSFETHGAPREI
                     YEFIYMLHTGEGLWWLGLLLGLSALTVPVLAVTGIQIWWKRRRGLPKLVGNVAADAAD
                     TVILVGTEGNTTWGFARTLHDAMTKAGHRVHTAPMNQLAPAYRSATRLFILAATYGDG
                     GPPASGNQFLAKLRKFNGAPQFQFTVLGFGDRQFPRFCQFAVDVDAALHAKGWTAFCP
                     LDLIDRQSPQE"
BASE COUNT          484 a          727 c          779 g          484 t
ORIGIN      
        1 gagcctgaac accccggcct gcttttttaa ccggatttca ggttgctgtc aggataccgc
       61 actggtcttt ggttccgggc caggcggccc tgccaaatta ccatccccat cacccgttcc
      121 tttgatctgc cttcagactg ccctaaggaa ggaggattaa agtagtcggc agcaaagaaa
      181 aaggagagtg aacatgaaag gcaagctgct cgcccttggc atcttttcca ttctgatgac
      241 cggcgtcgca caggcggatg atcattgcga tgaccccgtc gctgactggc agccacgcga
      301 tgcgctccgg catgaactcg agagcaacgg ctggcaggtc aagcgcatcc gggtcgacga
      361 cggttgctat gaagtggatg gcattgacag caatggcaac cggttcgaag ccacgttcac
      421 accggcatcg ctggagatcc gcgagctgga aatccgtttt cgcaaaggtg gtcgcgcggg
      481 ggattacctg gacaaggacc ggccgcacca ccggcacggg gctccggcgg gcacgcctgc
      541 cagtccgtga tccagtctgg ctgcatcgat caaatgtaat gagcaatgga gaggacatcc
      601 catgaaaacc aaacaattgt ttctggccct cggtaccgcg ctggcattgc cggcactgtc
      661 gctggccaaa cccgtcaccc tgaccacgga gctgtcggcc tacagcggtg atggcgccta
      721 tgtggcgctg tatctcaccg atgccaatgg gcagtacaag cgcacgctgt ggattgccgg
      781 caaaaagccg aaatactaca aacatctggc ggactgggcc cgtggcagcg gccagaacaa
      841 agccgaatac gatggcatga ccggcgccag cgttcacagt ggcaaaaccc tgtcggtgac
      901 agtagaggtt cctgatgcac tgatcgatgc cggtaaccag attcacatcg acactgcggt
      961 tgagaaaaag cgcgacaacc gatccgaagt ggtggtgccg ctgacgaccg atggcgccgg
     1021 caaacccact gcaggcaagg gttacgtgaa ggcgttcacc tacagcttct aagtctggaa
     1081 ttgcggaaac atgctacgtc aattgcattc cctgcccggt ctggttgccg cggtgttggt
     1141 ggccgtgctg gccgtgagcg gggcagttct ttccatcagt cctgccctgg aacggtggag
     1201 cgcgaccttg ccggccaccg gccagatgac ggtggcagac ctggcgggaa aggtggcaac
     1261 gcaatatccg ctggtggagc agatccagcg tacgccgtcc ggttcgatca tcgtgtattt
     1321 ccgggatggc gacgtttcgg gcgtggacct gtttgatccg gtcgccggca aatcgctgtc
     1381 cgcatacgaa agcccgtcct tgatgcgctg ggtgaggaat ctgcaccggt cgctgttgct
     1441 ggacacggaa ggccggattg caaccgggac aggcgcagtg ctgatggttc tgctggtgat
     1501 gaccggctcg ttcctgctgg ctgcacgcct gggcggctgg cgcaacattc tgggcccggt
     1561 gcgcggctcg ttgtcccagc gactgcatgg aatcctgagc cgctatgcca tgctcggcct
     1621 gctgctttct gcgatgaccg ggatgtatct gtcggcaggc agcctggaac ttattcccga
     1681 ggcactggaa gcggagccgg aatttcccga gtccggcgag ctggcggcac cactgcctgt
     1741 gcacacgctg gcggcactca aggcaatcga tctgaatgac ctgcgggaaa tggtttaccc
     1801 ctacccgggc gatcccgccg acaattattc cgtgcgcacg cgccagggcg caggcttcgt
     1861 gcatgcgtct tccggcgaac tgctgtcctt tgagacgcac ggcgcgccgc gcgaaatata
     1921 cgaattcatt tacatgctgc ataccggcga agggttgtgg tggctgggcc tgctgctggg
     1981 tctgtcggcg ctgacagtgc cggtgctggc agtgacgggc atccagatct ggtggaagcg
     2041 gcggcggggc ctgcccaaac tggtgggcaa tgtcgcggcg gacgccgccg atacggtgat
     2101 tctggtgggc actgagggca acactacctg gggcttcgcc agaaccctgc acgatgcgat
     2161 gacgaaggca ggtcatcgcg tccacacggc acccatgaac cagctggcgc ccgcatatcg
     2221 cagtgcgacg cggctgttta tcctggcggc cacgtatggc gacggtggtc cgccagcgtc
     2281 tggcaaccag tttctggcga agctgcgaaa attcaacggt gcaccgcaat tccagttcac
     2341 cgtactgggt tttggcgacc ggcagtttcc caggttctgc cagttcgccg ttgatgtcga
     2401 cgcagcactg cacgccaaag gctggacggc cttctgcccg ctcgacctga ttgaccggca
     2461 atcgccgcag gaat
//
LOCUS       DASUEG010000855         4666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1859461, whole genome shotgun sequence.
ACCESSION   DASUEG010000855 DASUEG010000000
VERSION     DASUEG010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859461"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFX11_16105"
     CDS             <1..426
                     /locus_tag="VFX11_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940202.1"
                     /translation="VYTLGFENQDGVTVDYVTDIGPLNSRLTLMAGRSDSTVESNGDL
                     LDVSIRNQMGGAWSLTYDWLTARVAYFESRLAIPNEGTATLADGIIGYDAGLALVVPG
                     YQVGTLDSVADDVQFDEDKASFAGIGVSADVNNIIAVAE"
     gene            430..804
                     /locus_tag="VFX11_16110"
     CDS             430..804
                     /locus_tag="VFX11_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4940203.1"
                     /translation="MVRSDETPLEDRNSWYASLGYRIQKFTVFGTFEKWDFNAQTDTI
                     EEFERSAPITGIGPVDAGRAGILAGLEQTFVGTKDADTVGVGLRYDFHPSAAFKVEYN
                     QMDNQTDDTKPKSLAVAVQLVF"
     gene            818..1240
                     /locus_tag="VFX11_16115"
     CDS             818..1240
                     /locus_tag="VFX11_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009838694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4940204.1"
                     /translation="MKSNVKKVLCASLLTIFCSATHAEVAVIVNPANADAITKDDIAS
                     LYLAKTKTFPGGKNAIPLDRPEGSPTRVEFVSKVIDKEEAQMKSYWSRLIFTGKGVPP
                     KVVETDAEVKDMVARNPDTIGFIDAAAADATVKVVAKF"
     gene            complement(1327..2652)
                     /gene="pmbA"
                     /locus_tag="VFX11_16120"
     CDS             complement(1327..2652)
                     /gene="pmbA"
                     /locus_tag="VFX11_16120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX4940205.1"
                     /translation="MTITRDQQLLRDCISQALELAGKAGASAEVGVYQDQGLLTSVRQ
                     GQVDKVEFTRNHGFAVTVYRGRSKGHASSSDFSSTAIEQTVQAALDIARFTAEDPCGG
                     LADADQMARSIPDLDLYHPWDLQPQQAIELARQCEAAGLQAHPAIANSDGASVASGES
                     LRGYGNSHGLVVVYPQSRHAVSCALIAKQGDRMERGGWSFSHRDSRVLPAVDGIGIRA
                     AERASRRLGSRSVPTCDVPVLFAAEIAGGLLGHFISAISGGNLYRHASFLEGALGQRV
                     FPSWMTISEHPLLPCEIGSAPVDGDGLPTSEKAFVRDGELASYVLGTYSARKLGLRST
                     ANAGGVRNLRCSSTHSLDDLLQQMGTGLLVTDVMGQGINILTGDYSRGAAGFWVENGA
                     LQFPVSEITIAGNLKSMFGNLVGVGTDIDNRGNVQTGSILLASMKVGGK"
     gene            2888..>4666
                     /locus_tag="VFX11_16125"
     CDS             2888..>4666
                     /locus_tag="VFX11_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4940206.1"
                     /translation="MRRPAADAGLPVIPRFKLLKKLGEGGMASVFLGRQESTGREVAI
                     KILAHHLQSNARWKDRFLDEARRLAELSHPNVVPVFDWGTHEGVGYIVMEYIRGGTLA
                     QRLKTQTVTVRDAIQITQQIAAGLDFASEKGYVHRDIKPDNILFREDGSLCILDFGIA
                     KESESNTTITSQGIPFGTGAYMSPEQAQPSGKVIDGRSDLYSLGIVLYEILTGTRPFD
                     FRQYDAMKGFQMYLFAHVHSPPPPLPPAVSAFQPVLNQLLAKEPKDRFSRGNDVREAL
                     ARLAATLPEQELARGIRGPGESTLLMSGPAGLHPDATPVAQHAGVNSAPGHAAEPARV
                     GPEHPSGVAISLPDVPRKYARKAWTLAGAVAITGLSAGYLTLAPKSPAPDNTPVPAQA
                     EANTPTPQPAAVKSGSATTDETDRITRVITQQLQAARELMQAEPGNLMAQQQLVARLN
                     QILQLAPGHAEATTSLERVLQKQVAHTRAQLDSNRLDTVPAHLSLIASLSREAAADLQ
                     SRYDAARKAEADNRQRLIRLAGIEQDIQRQLTTAQKQQPTQARETLLNTAGLTRDAMA
                     IQLGEEEATRYRQQIASAYTDRIQQQI"
BASE COUNT          956 a         1511 c         1383 g          816 t
ORIGIN      
        1 gtttacaccc tgggtttcga gaaccaggac ggggtgaccg tcgattatgt taccgacatc
       61 ggcccattga attcccgcct gaccctgatg gcagggcgct cggattcgac tgtggaaagc
      121 aacggggact tgctggacgt cagcatccgc aaccagatgg gtggtgcctg gagcctgacc
      181 tacgactggc tcaccgcgcg ggtagcctat ttcgagtcgc ggctggcgat tccaaatgag
      241 ggtactgcca ctcttgccga tggcattatt ggctatgatg ccggactcgc gctcgtagtg
      301 cccgggtatc aggttggaac gctggacagc gtggctgacg acgtgcaatt cgacgaggac
      361 aaggcttcct tcgcgggtat tggtgtaagc gccgatgtga acaacatcat tgcggtagct
      421 gaataaacca tggtccgcag cgatgaaact ccgctggaag atcgcaattc ctggtatgca
      481 tctcttggat atcgcatcca gaaattcacg gtgttcggta ctttcgaaaa atgggacttc
      541 aacgcacaga ccgacaccat cgaagaattt gaacgttctg caccgatcac cggcattggt
      601 cccgtcgacg ccggtcgcgc cggcatcctg gctggcctgg agcaaacctt tgtcggcacc
      661 aaggacgctg acaccgttgg cgttggcctg cgttacgatt tccatccttc agctgcattc
      721 aaggtggaat acaaccagat ggataaccag actgacgata ccaaaccgaa gtcgctggcc
      781 gtcgccgtgc aactggtgtt ctaagggagg agccgacatg aaatccaatg ttaaaaaagt
      841 gctgtgcgcg tccctgctga ccattttctg tagtgctacg catgccgaag ttgcagtaat
      901 cgtcaatcct gccaacgccg acgccattac caaggatgat attgcttccc tttacctggc
      961 caaaaccaaa accttccccg gcggcaagaa tgcaattccg ctggatcgtc ccgaaggctc
     1021 gcccacccgg gtggaattcg tctccaaggt gatcgacaaa gaagaggctc aaatgaagtc
     1081 ctactggtcg cgcctgattt tcaccggtaa gggcgtacca cccaaggtgg tggaaacgga
     1141 tgcggaagtg aaggacatgg tggcacgcaa tcccgatacc atcggcttca tcgatgcggc
     1201 ggcggcagat gctaccgtga aggtagtagc gaaattctga gtttgtttcc gacttttagc
     1261 gatcctgttt tatgttgatc ggccccgggc gtaacaaccc ggggcttttt tcatccaccg
     1321 ctgtcgttat ttcccgccca ccttcatcga tgccagcagg atcgagccgg tctgcacgtt
     1381 gccacggtta tcgatatccg tacccacacc caccagattc ccgaacatgg atttcagatt
     1441 gcccgcgatg gtgatctcgg ataccggaaa ctggagtgca ccattttcca cccagaatcc
     1501 cgccgcgccc cgggaataat caccagtaag aatgttgatg ccctgcccca tgacatccgt
     1561 caccagcaga ccggtgccca tttgttgcag cagatcgtcc aggctgtggg tggagctaca
     1621 gcgcaggttg cgcacgccgc cggcattggc ggtgctgcgc agccccagtt tgcgggcgga
     1681 gtaggtgccc agcacataac tggccagctc gccatcacgg acgaaggcct tttccgacgt
     1741 gggcaggcca tcgccatcca ccggcgcgct accgatttcg cagggcagca acggatgctc
     1801 gctgatggtc atccaggacg ggaagacgcg ctgccccagc gccccttcca gaaacgaggc
     1861 atgccggtag aggttgccgc cggaaatggc gctgatgaaa tgccccagca gcccgccagc
     1921 gatttccgcc gcgaacagta ccggcacgtc gcaggtgggc acgctgcggc tgcccaggcg
     1981 ccggctggcg cgttccgctg cccgaatgcc gatgccgtcc accgccggca gaacgcggga
     2041 atcccggtgg ctgaacgacc agccgccccg ctccatcctg tccccctgtt tggcgatcag
     2101 cgcgcagctg acggcatggc gcgattgcgg gtacaccacc accagaccat gactgttgcc
     2161 gtaaccccgc agggattcgc cgctggccac actggcgcca tcactgttgg caatggcggg
     2221 atgcgcctgc aggcccgccg cctcgcactg ccgcgccagc tcgatggcct gttgcggctg
     2281 cagatcccag ggatggtaaa gatccaggtc cggaatggag cgcgccatct ggtcggcatc
     2341 cgccagcccg ccgcaggggt cttccgctgt gaaacgggcg atgtccaggg ccgcctgcac
     2401 cgtctgctca atggcggtag aactgaaatc cgaactcgat gcatggccct tgctgcgccc
     2461 ccgatacaca gtgacggcaa agccgtggtt gcgggtgaac tccaccttgt ccacctgccc
     2521 ctgccgcacc gaggtcagca ggccctggtc ctggtacacc cccacttccg cactggcccc
     2581 ggccttgccc gccagctcca gcgcctggga gatgcagtca cgcaacagct gctgatcacg
     2641 ggtgatcgtc atgagaaggg aatccttagc cgtcggaccg gcgaaacaca ctgttatgga
     2701 aggggctgtg gacggcaggc ggtgtccgtt ggtcaccctg cattccagcg gaatttggtg
     2761 gtaccatggc tgccctgtgc aactgcgcgt gcaagcctgt aatccccgga ataatagcaa
     2821 tggtgctggc ctgatgtctg ccaaagagcg aagtaacctg ttcttgcagg cgtggctacg
     2881 cagactcttg cgccgtccgg ccgccgatgc cgggctgccg gtcatccccc gattcaaact
     2941 gctgaaaaaa ctgggcgaag gcggcatggc cagcgtcttt cttggccgcc aggaatccac
     3001 cggccgcgag gtcgcgatca agatcctggc ccaccatctg caaagcaacg cccgctggaa
     3061 agaccgcttc ctggacgagg cccgccgtct ggcggagctg tcccacccca atgtggtacc
     3121 cgtgttcgac tggggcaccc acgagggcgt cggctacatc gtcatggaat acatcagggg
     3181 cggcaccctg gcccagcgcc tgaaaaccca gaccgtcacg gtgcgcgacg ccatccagat
     3241 cacccagcag atcgccgccg gactggattt cgccagcgag aaaggctacg tgcaccggga
     3301 catcaagccg gacaacatcc tgttccgcga ggacggctcc ctctgcatcc tcgatttcgg
     3361 cattgccaag gaaagcgaat ccaacaccac catcaccagc cagggcatcc ccttcggcac
     3421 cggcgcctac atgagccccg agcaggccca gccgtcgggc aaggtgattg atggccgcag
     3481 cgatctgtac agcctgggga tcgtactgta cgaaatcctc accggcacgc ggccgtttga
     3541 tttccgccag tacgatgcga tgaaagggtt ccagatgtac ctgtttgcgc acgtgcattc
     3601 gcccccaccg cccctgccac cggccgtgtc ggcctttcag ccggtgctca accagctgct
     3661 ggccaaggaa cccaaagacc gcttcagtcg cggcaacgat gtgcgggaag cactggcgcg
     3721 cctggcggcg actctgccgg agcaggaact ggcccgcggc atacgggggc cgggagagag
     3781 cacgctgctg atgtcggggc ctgcggggct gcatccggat gcgaccccgg tggcgcagca
     3841 cgcgggtgtg aatagcgcgc caggtcatgc agctgagccg gcccgtgttg ggccggaaca
     3901 tcccagcgga gtggccatca gtctgccgga cgtgcccagg aaatatgcgc gcaaagcctg
     3961 gacactggcg ggcgcggtgg ccatcacggg gctgtcggcg ggttatctga cgctggcacc
     4021 caaatcaccc gcccctgaca acacaccggt gccggcccag gccgaagcca atacgcccac
     4081 accgcaaccg gcggcggtga aaagcggctc tgcgacaact gacgaaacgg atcgcatcac
     4141 ccgcgtcatc acccagcagt tgcaggccgc ccgggagctg atgcaggccg aacccggcaa
     4201 cctgatggcc cagcaacagc tggtggcgcg cctcaaccag atactgcaac tggcgcccgg
     4261 ccacgccgaa gccactacca gcctggaacg cgtgctgcag aagcaggtgg cccacacccg
     4321 cgcccaactg gacagcaacc gactggatac agtgcccgct cacctgagcc tgatcgccag
     4381 cctgtcccgc gaggcagcgg cggacctgca atcccgctac gacgccgccc gcaaggccga
     4441 ggccgacaac cggcagcgcc tgatccggct cgccggtatt gaacaggata ttcagcgcca
     4501 actgacaacg gcacaaaaac agcagcccac ccaggccagg gagacactgc tcaacactgc
     4561 cgggctgacc cgggatgcca tggccattca actgggggaa gaagaggcca cgcgctaccg
     4621 ccagcagatc gccagcgcct ataccgaccg gattcagcag cagatc
//
LOCUS       DASUEG010000856         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1867147, whole genome shotgun sequence.
ACCESSION   DASUEG010000856 DASUEG010000000
VERSION     DASUEG010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867147"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFX11_16130"
     CDS             complement(<1..887)
                     /locus_tag="VFX11_16130"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX4940207.1"
                     /translation="MSDEPLQEALVKPSKSLSIIWLLPLIAAAIGAWLLIKSVKEAPV
                     EITINFPSGTGIEVGKTKVIHEGITAGVVSDVRLDQSDMTGVIATLDMDRRVEPLLRT
                     TTQFWLVKPEISLSGVTGLETIVTGNYIGVKIGLDGERTRHFDALEKPPVLDSKVPGL
                     HLKLLAKDLGSIYTGAPVMFKKIVVGDVQEYELDAANDRVVISVHIRPPYDQLVTEKT
                     RFWNASGFRVKAGLDGVDMRMDSLLSLVKGGITFDVTDPARETPAKNGDEFRLYRDYG
                     SAQRGVRATIRFASAEKPA"
     gene            complement(880..1509)
                     /locus_tag="VFX11_16135"
     CDS             complement(880..1509)
                     /locus_tag="VFX11_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX4940208.1"
                     /translation="MVVQLAPQLQQGQSCCPRCGSVLHARMPNSIARTWALVITAWIF
                     ILPANLYPVMTVIYFGEGEPDTILSGVVHLAQAGMIPIAILVFVASIVVPVLKLVGIM
                     VLLLTVQFKWQLNKRQCTVLYRVIEVIGAWSMLDLFMISILVTLVDLGAVATITAGTG
                     ATAFATVVVLTMFAAMSFDPRLIWDLEDGLPEPPALHPQPNLPQEPPHV"
     gene            complement(1544..2173)
                     /locus_tag="VFX11_16140"
     CDS             complement(1544..2173)
                     /locus_tag="VFX11_16140"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX4940209.1"
                     /translation="MLHHQSQLVCHECDALLQATHADPGQKLVCPRCGCTLYKPRRDS
                     INRGLALSLTALILFVPANFLPIMTFNMLGLNNADTMVKGATQLFRQGYWWMAALVFF
                     CSILAPLLEFVLITGICLLVKLNRYNAPLVFMLKIQSRVNRWGMLEVFMLGILVAYIK
                     MIDLGEIQLGMGMVCFTGLLAVTTLNAVLFDTHPIWEKVGQARARLSDR"
     gene            complement(2330..>5313)
                     /locus_tag="VFX11_16145"
     CDS             complement(2330..>5313)
                     /locus_tag="VFX11_16145"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX4940210.1"
                     /translation="QGSDQGLGFTDSWRFNVQETGMEILEFGYTTISTDGSADYAWTG
                     SDWTLRDRTTWMLSATGWNEFADAPSVDGTPATTAILARGTTGNYGSRWEMAGYASAI
                     TGLPMREFPGEEWEEGLADPAANFAGDNARGVGVAVTSLMDEYEILCDLNMPKYGLLQ
                     CENWILQSFPVSGNDQDVTTDNLADALSDVLHANGSVPATPIGGVPVGRIYNGNSETH
                     VFAWLTGSNVSGSAGTGGIVEFYTHTAPNMTSQKIANVAADWNITDPSGTGALVLRFT
                     LPDAIHADEFFADYEMHRNVVLAAVALADANPYLRAGHFSPSGTVQQHTALNGVALNE
                     LLTGFAYQKPDTDADGVSDDMDNCPAVANADQLDENGNGRGDACEGGGGTDSDNDTVP
                     DVSDNCPMVPNQNQNNLDGDAQGDACDIDLDGDGINNPTDNCPMMPNADQTDTDTDGI
                     GDVCDNGGGGSLVDTDSDGVIDSQDAFPDNASEQRDSDGDGTGDNSDACPYTASATCA
                     DPGVNMQGVYLASITATGLEWDESTQQCVTITNGSRAHLLKFRQLGNQLVMSGEDENG
                     SWEDYGTIAANGQFQIQNVADGFQLSGTFVAGSVFNVSYTDNADGCAQSGTGTFNPGA
                     DIAENSVGDVGLVHFDGDSFTDGASGIEVLEFEYGIISSLALEQRFEYDLDTSAWVDV
                     STFPGLEYFITDVGIESVQDRYRISGYVNAGQTAIGQPATSDGLALSSLTSLHVDLRE
                     LNVENLPMLPLLGGTYGAAIAPDSLFATGARAYFATLTQQVSLYSFWCDDDWNQYVVD
                     TYTGCQNVVAKSYQDFNTDGQMDPVAATALDEVVSTPADFNSGTVGGTGLWLGDGEDV
                     VSGVGGSEYHPYYLNGYLISDNGNASGANPVLRIVKHYQNTTNYWAEKVVIAEVPFTA
                     TTVNGVNVIQWDTPARVMQLGHLDSEQAHPFLFEESALDSQPLVRRGERQISGTVEHE
                     LLYNLTAKDQILNAFEIVPGP"
BASE COUNT          995 a         1610 c         1658 g         1050 t
ORIGIN      
        1 tccgccggct tttcggccga ggcgaagcgg atggtggcac gcacgccgcg ctgggcactg
       61 ccgtaatccc gatacagacg gaattcgtcg ccatttttgg caggggtctc cctggcggga
      121 tcggtgacat cgaaggtgat gccgcccttc accagcgaca gcagcgaatc catgcgcatg
      181 tcgacaccgt cgaggcccgc tttcacgcgg aaaccgctgg cgttccagaa gcgggttttc
      241 tccgtcacca gctggtcgta gggcgggcgg atatgcacgc tgatgacaac gcggtcattg
      301 gcggcatcca gttcgtattc ctgcacgtcg cccaccacga ttttcttgaa catcaccggc
      361 gcgcccgtgt agatcgagcc cagatccttc gccagcagtt tcaggtgcag gcccggcacc
      421 ttgctgtcca gcaccggcgg tttttccagt gcgtcgaaat ggcgggtgcg ttcgccgtcc
      481 aggccgattt tcacgccgat gtaattgccg gtgacgatgg tttccagtcc ggtgacacca
      541 ctcaggctga tctccggttt caccagccag aactgtgtgg tggtgcgcag cagcggttcg
      601 acacggcggt ccatgtcgag ggtggcgatg acgccggtca tgtcgctctg gtcgaggcgc
      661 acgtcgctca ccacgccggc ggtgatgccc tcgtgaatga ccttggtctt gcccacttca
      721 atgccggtgc cgctggggaa attgatggtg atttccaccg gcgcctcttt cacggacttg
      781 atcagcaacc aggcgccgat ggcggccgca atcagcggca gcagccagat gatcgacagg
      841 cttttcgaag gcttgaccag cgcttcctgc aacggttcgt cagacatggg gtggttcctg
      901 tggcaaattc ggttggggat gcaaagccgg cggttccggc aggccgtctt ccagatccca
      961 gatcagacgg ggatcgaaac tcatggcggc gaacatggtg agcaccacca cggtggcgaa
     1021 ggccgtggcg ccggtaccgg cggtgatggt ggcgacggcc cccagatcca ccagcgtcac
     1081 cagaatggaa atcatgaaca ggtcgagcat ggaccaggcg ccgatcactt cgatgacgcg
     1141 gtaaagcacc gtgcactggc gcttgttgag ctgccatttg aactgcaccg tgagcagcag
     1201 caccatgatg cccaccagtt tcagcaccgg caccacaatg ctggcaacaa acaccagaat
     1261 ggcgattgga atcatgcccg cctgcgccag atgcaccacg ccggacagga tggtgtccgg
     1321 ctcgccttcg ccaaaataga tcaccgtcat caccggatac aggttggccg gcagaatgaa
     1381 aatccaggcg gtgatcacca gcgcccaggt gcgggcgatg ctgttgggca tgcgcgcgtg
     1441 cagcacgctg ccgcagcggg gacagcagga ctggccctgc tgcagctgcg gcgccagctg
     1501 taccaccaga tggcagtcgc ggcacagcac cagatggcgg gatctagcgg tcggaaaggc
     1561 gtgcacgggc ctgccccact ttttcccaga tgggatgggt atcgaacaga acggcgttca
     1621 gcgtggtaac ggcgagcagg ccggtgaaac agaccatgcc catgccaagc tggatttccc
     1681 ccaggtcgat catcttgatg taggccacca ggatgcccag catgaacact tccagcatgc
     1741 cccagcggtt gacgcgactc tggatcttca gcatgaacac cagcggtgcg ttgtagcggt
     1801 tcagtttcac cagcagacag atgccggtga tcaggacaaa ttccagcagc ggtgccagaa
     1861 tgctgcagaa aaacaccagc gccgccatcc accagtaacc ctgccggaac agctgggtgg
     1921 cacccttgac catggtgtcg gcattgttca ggcccagcat gttgaaggtc atgatcggca
     1981 ggaagttggc gggcacgaac aggatcagcg cggtgagact gagcgcgagg ccacgattga
     2041 tggaatcgcg gcgcggcttg tacagggtgc agccacagcg cggacacacc agtttctggc
     2101 ccggatcggc atgcgtggcc tgcaacagcg cgtcgcattc gtgacagacc agttgggatt
     2161 ggtggtgcag catgggccag gttgctcgcg ggaacagttg cggggatttt atcccagtcg
     2221 ggcgggcatg tctgtgcggg tgtgtgagtg cgggtgtgcc ggtgcggggt ttccaaaaaa
     2281 gaagccgccc ttgcaggcgg cttcgtggtg gctgatcccc cgacagcaat cagggcccgg
     2341 ggacaatctc gaaggcattc aggatctggt ccttcgcggt gaggttgtac agcagctcat
     2401 gctccacagt accgctgatc tggcgctcgc cgcgacgcac cagaggctgg ctgtcgagtg
     2461 ccgactcctc gaacaggaac gggtgcgcct gctcgctgtc cagatggccc aactgcatca
     2521 cgcgggctgg agtgtcccac tggatcacat tgacaccgtt cacggtggtg gcggtgaacg
     2581 gcacttccgc gatcaccact ttctccgccc agtagttggt ggtgttctgg taatgcttga
     2641 cgatgcgcag caccggattg gcaccgctgg cattgccgtt gtcactgatc aggtagccgt
     2701 tgaggtagta gggatggtac tcgctgccgc cgacgccgct caccacatcc tcgccatcac
     2761 ccagccacag gccagtacca ccgacggtgc cgctgttgaa gtcggccggt gtggatacca
     2821 cctcatccag cgccgtcgca gccaccgggt ccatctgtcc gtctgtgttg aaatcctgat
     2881 aggacttggc tactacgttc tggcatccgg tgtaggtatc caccacgtac tggttccagt
     2941 catcgtcaca ccagaaactg tacagcgaca cctgctgggt gagggtggcg aaataggccc
     3001 gggcaccggt ggcgaacagg ctgtccggcg cgatggcggc accgtaggta ccacccagca
     3061 gcggcaacat gggcaggttt tccacgttca gctcgcgcag gtccacatgc aggctggtga
     3121 gcgaggacag ggccagtccg tcgctggtgg cgggctggcc aatagcggtc tggccggcat
     3181 tgacgtaacc gctgatgcga taacggtcct gcacggattc aatgcccacg tcggtgatga
     3241 aatattccag acccgggaaa gtgctgacat ccacccaggc gctggtgtcc aggtcgtatt
     3301 cgaaacgctg ctccagggcc aggctggaaa tgatgccgta ttcgaactcc agcacttcga
     3361 tgccgctggc accgtcagtg aagctgtcgc catcgaaatg caccaggccc acatcgccca
     3421 cgctgttttc ggcgatgtcc gcgcccggat tgaaggtgcc ggtgccactc tgcgcgcagc
     3481 catcggcgtt gtcggtgtag ctgacattga acactgagcc ggctacaaag gtaccgctca
     3541 actggaagcc atcggcaacg ttctggatct ggaactggcc gttggcagcg atggtgccgt
     3601 aatcctccca gctgccgttt tcatcctcac cgctcatcac cagctggttg cccagctgac
     3661 ggaacttcag gagatgcgca cggctgccgt tggtgatggt cacgcattgc tgcgtcgact
     3721 catcccactc cagaccggtg gcggtaatgc tggccagata gaccccctgc atgttcacgc
     3781 cgggatcggc acaggtggcg ctggcggtgt aggggcaggc gtcgctgttg tcgcctgtac
     3841 cgtcaccgtc gctgtcgcgc tgctcactgg cgttgtcagg gaaggcgtcc tgactgtcga
     3901 tcacgccatc gctgtcggtg tccaccaggg aaccaccgcc accgttgtcg cagacatcgc
     3961 cgatgccatc cgtgtcagta tcggtctgat cggcgttggg catcatgggg cagttgtcgg
     4021 tgggattgtt gatgccgtca ccatccagat cgatgtcaca ggcgtcaccc tgggcgtcgc
     4081 catccagatt gttctggttc tggttgggca ccatggggca gttgtcgctg acatccggca
     4141 cggtgtcatt gtcgctatcg gttccaccgc cgccttcgca ggcatcaccg cgaccattgc
     4201 cgttttcatc gagctgatcg gcgttggcaa cagccggaca gttgtccatg tcatcgctga
     4261 cgccatctgc atcggtgtcc ggtttctggt aagcgaagcc ggtgagcagt tcgttcagcg
     4321 ccacgccgtt cagcgcggtg tgctgctgca cggtgccgct gggcgagaag tgcccggcgc
     4381 gcaggtacgg attggcatcg gccaacgcca ccgccgccag caccacgttg cgatgcattt
     4441 cgtaatcagc gaagaactcg tcggcgtgga tggcatccgg aagggtgaag cgcaacacca
     4501 gcgcaccggt gccggacgga tcagtgatat tccagtcagc ggcaacattt gcaattttct
     4561 gcgaggtcat gttgggcgcc gtgtgcgtgt aaaactccac aatgccaccc gtaccggcac
     4621 tgccactgac gttgctgccg gtcagccagg cgaacacatg ggtttcactg ttgccgttgt
     4681 aaatacgacc cacgggcact ccgccgatgg gtgtagccgg cacggaaccg ttggcatgca
     4741 acacatcact cagcgcgtcc gccaggttgt cggtagtgac atcctgatcg ttgcccgaga
     4801 cggggaagct ctgcaggatc cagttttcac actgcagcag cccgtatttt ggcatgttca
     4861 ggtcgcacag gatttcgtac tcatccatca gcgacgtcac cgccacacct acgccacgcg
     4921 cgttgtcgcc ggcaaagttg gcagcaggat cggccaggcc ttcctcccat tcttcgccgg
     4981 ggaattcacg catgggcaga ccggtgatgg cgctggcata acctgccatt tcccaacggc
     5041 tgccgtaatt gccggtggtg ccacgggcca gaatcgcggt ggtggccggt gttccgtcca
     5101 ctgacggcgc atcggcgaac tcgttccagc cggtggcgct cagcatccag gtggtgcggt
     5161 cgcgcagcgt ccagtcgctg ccggtccacg cgtaatctgc gctgccgtca gtggaaatgg
     5221 tggtgtagcc aaactccagg atttccatgc cggtttcctg aacattgaag cgccagctgt
     5281 cggtgaagcc cagaccctga tcgctaccct gcg
//
LOCUS       DASUEG010000857         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1646304, whole genome shotgun sequence.
ACCESSION   DASUEG010000857 DASUEG010000000
VERSION     DASUEG010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646304"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1591
                     /locus_tag="VFX11_16150"
     CDS             443..1591
                     /locus_tag="VFX11_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940211.1"
                     /translation="MKKIVSAVVIVLLVAGIYWFSSRPEIPQVGVAEIGQGEVESLVS
                     NTRAGTIRSCQRSKLSLQSGGTVAELLVKNGDRVQQGQVLLRLWNGDQKARLSQARAQ
                     LDSANLAVREICGNAARDQRDAKRAVTLASKKVISEDALDTSRTRAEVSSFSCERAKV
                     DASAAQAQLELQQALLQQTELVAPFAGVVAEINGEVGEFVTPSPPGVATPPAVDLIAD
                     ECLYVRAPIDEVDAAAIKVGMPARLSLDAFRGQTFAGTVSRIAPYVQDYEKQARTVDV
                     EARFDQPPANVQLLVGYSADIEVILNRHETVLRVPTEAVFDGNQVLVLNSNNVLEKRE
                     FETGLANWTWTEVKSGLQAGDRVLLTLDTPGAVDGAEVAPTPQGKAND"
     gene            1584..2264
                     /locus_tag="VFX11_16155"
     CDS             1584..2264
                     /locus_tag="VFX11_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4940212.1"
                     /translation="MIELQHINKTFQLGDQTVHALDDVSLLIDKGEYISVMGPSGSGK
                     STLLNVLGLLDQPNDGHYLLEGTDTTQLSEPERARYRRDHIGFVFQSYHLVNRLNARE
                     NIELPLVLAGIPPKERAPRVDEMLQRLSLTPRADHLPNQLSGGQRQRVAIGRAIIMKP
                     QILLADEPTGNLDSKSGADVIELLEELNVQQGITLLIVTHDAAVGQRAHRRIRMVDGR
                     IDTDKNGA"
     gene            2268..>2841
                     /locus_tag="VFX11_16160"
     CDS             2268..>2841
                     /locus_tag="VFX11_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008224553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4940213.1"
                     /translation="MRTADLLRFDFQVLNRHRFRALMMLIAMSIGVAAVNLLTGLGEG
                     AKAFVLGEFSMLGKNTLIMLPGKKETSGGMPPMMGAAPRDITLQDAEAMRRLSGVVQI
                     APLIVGMSEVSYSSRNRDVMIAGTTASYFSIRQLTIRQGTALPEAPSDVASPVCVIGW
                     KVKQEIFGERPALGHWLKAGDRRFRVVGIMD"
BASE COUNT          575 a          853 c          898 g          515 t
ORIGIN      
        1 atccgcctct gccgatgccg ccgcgggcag tgacaatccg gccagcccca ttgcaaaggc
       61 cagacccagc gccggcaagc gcagcatgga tcggaacagg ctcgtcatta tccccatggg
      121 accgtcagtc gctcgtgatc aggtatcagc aaacaatctt atagttctgt acaaaaatta
      181 gacaggtcgc agacctattg gtagcaggtt cctgtgtcga tttttatcgg tttgtaagtg
      241 gttgaaccag ttccgcgaac caggtcaacc attatagtca ccactgcatt cctcaccgga
      301 tttttatgac cagcggcaac ccgccgccct ttgcgcccct aacttgacgc aagccagtca
      361 caagcaaggc atttctggtt agagtagcgg ttgttttgcg ccgggctgca gtgcgctgaa
      421 tcgcaccgac taaggatcct ccatgaaaaa gattgtctct gccgttgtca tcgttttgct
      481 ggttgccggc atttactggt tcagcagccg ccccgagatt ccccaggtcg gcgtggcgga
      541 aatcggacag ggcgaggtgg aatcactggt gtccaacacc cgggcaggca ccatccggtc
      601 ctgccagcgt tcgaagctgt cgctgcaatc ggggggcacg gtggcggaac tgctggtgaa
      661 gaacggtgac cgggtgcagc agggtcaggt tctgctacga ctgtggaacg gtgaccagaa
      721 ggcacggttg agtcaggcca gggcgcaact ggattctgcc aacctggcgg tgagggaaat
      781 ctgcggcaac gccgcccgcg accagcgcga tgccaagcgg gcggtaacgc tggcgagcaa
      841 aaaggtgatc tcggaggatg cgctggatac ctcgcgcacc cgggcggaag tgtccagttt
      901 cagctgcgag agggcgaagg tggacgccag cgcggcccag gcccagctgg aactgcagca
      961 ggcgctgttg cagcagaccg aactggtggc gccgttcgct ggcgtggtgg cggagatcaa
     1021 cggtgaagtc ggcgagttcg tcaccccttc cccaccgggc gttgccacgc caccggccgt
     1081 ggacctgatc gccgatgaat gcctgtacgt gcgcgcaccc atcgatgagg tggacgccgc
     1141 cgccatcaag gtgggcatgc ccgcccgcct cagcctggat gcctttcgtg gccagacttt
     1201 cgcgggcacc gtcagccgca ttgcgcctta tgttcaggac tacgaaaagc aggcccgcac
     1261 cgtggatgtg gaagcacgtt tcgaccagcc tccggccaac gtgcaattgc tggtgggtta
     1321 cagcgccgac atcgaagtga ttctgaaccg ccacgaaaca gtattgcgtg tccccaccga
     1381 agcggtgttc gacggtaatc aggtgctggt tttgaatagc aacaacgtgc tggaaaaacg
     1441 cgagtttgaa accggtctgg cgaactggac ctggaccgag gtgaaaagcg gcctgcaggc
     1501 gggcgacagg gtactgctga ccctcgatac gcccggcgcg gtggacggcg cggaagtggc
     1561 ccccacaccg caagggaagg ccaatgattg agctgcagca catcaacaaa acattccagc
     1621 tcggggacca gactgtccac gcgctggatg atgtcagtct gcttatcgac aagggcgaat
     1681 atatttccgt gatggggccg tccggttccg gcaagtccac cctgctcaac gtgctgggtc
     1741 tgctggacca gcccaacgac ggccactatc tgctggaagg caccgacacc acacaactga
     1801 gcgaaccgga gcgcgcccgc taccgccgcg atcatatcgg cttcgtgttt cagtcctatc
     1861 acctggtgaa ccggctcaac gcgcgcgaga acatcgagct gccgctggtg ctggcaggga
     1921 ttccaccgaa ggaacgcgcg ccaagagtgg atgaaatgct gcagcggctg agcctgactc
     1981 cgcgcgccga tcatttgccc aaccagctgt ccggcgggca gcgccagcgg gtggcgattg
     2041 gccgcgcgat tatcatgaaa ccgcagatcc tgctggcgga cgaacccacc ggcaacctgg
     2101 acagcaaatc cggcgccgac gtgatcgagc tgctggagga actgaacgtg caacagggca
     2161 tcacgcttct gatcgtcact cacgatgccg ccgtgggtca gcgcgctcac cgccgcatcc
     2221 gcatggtgga cggccgcatc gacactgaca agaacggagc ctgaacgatg cgcaccgccg
     2281 acctgcttcg cttcgacttc caggtgctca accgtcaccg ctttcgcgcg ctgatgatgt
     2341 tgattgccat gtcgatcggc gtggcggcgg tgaacctgct caccggcctg ggagaaggcg
     2401 ccaaggcgtt cgtgctgggg gaattttcca tgctcggaaa aaataccctg atcatgttgc
     2461 ccggcaagaa ggaaaccagt ggtggcatgc cgccgatgat gggcgccgcg ccgcgcgaca
     2521 ttaccttgca ggacgccgag gccatgcgcc ggctcagcgg cgtggtgcag attgcaccgc
     2581 tgattgtcgg catgagcgaa gtgtcgtaca gcagccgcaa ccgggatgtg atgatcgcgg
     2641 gcaccaccgc ctcgtacttt tccatccgcc agttgacgat tcgccagggc acggcgttgc
     2701 cggaagcgcc cagcgatgta gccagcccgg tgtgcgtgat cggctggaag gtgaagcagg
     2761 aaatcttcgg tgagcgcccc gccctgggcc attggctgaa agccggcgac cgccgtttcc
     2821 gcgtggtggg gatcatggat g
//
LOCUS       DASUEG010000858         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1482809, whole genome shotgun sequence.
ACCESSION   DASUEG010000858 DASUEG010000000
VERSION     DASUEG010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482809"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFX11_16165"
     CDS             <1..354
                     /locus_tag="VFX11_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940214.1"
                     /translation="QTPYATKHAFNGWVDQFLVTPDQGLNDKYVSLGFDLNAWTTKIL
                     IQYHEYESDVESGPLGKELDMGEESGVQILKTLGPNWTLGAKYGDYKQPSQEFTTGNT
                     RRNADKVWLWAELNF"
     gene            complement(457..1296)
                     /locus_tag="VFX11_16170"
     CDS             complement(457..1296)
                     /locus_tag="VFX11_16170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940215.1"
                     /translation="MDTSSISFTALYTGHVWYKHGMSARFFTSPRADLMYNAVRPINF
                     VGEQLLGMSLSDMLLQRHRIMDHRVRQLIEQEGVTQILEIACGLSPRGYTFSRQYPHL
                     HYVEADLPGMATRKQSLLLRHQGFGPNHKVVACNIFESGEPNGLDHVLANVLEPGRPT
                     IVITEGLVNYFDLATISGFWQKLAQLGRQFPAAWYITDLVPEVKEFHRRVLVSAAQKA
                     LGVMTQSRTNLHFRSDADVERGFRRCGFQSVTVHRPEDFYDALDIPRSEGESYVRVVE
                     ARC"
     gene            complement(1363..1677)
                     /locus_tag="VFX11_16175"
     CDS             complement(1363..1677)
                     /locus_tag="VFX11_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4940216.1"
                     /translation="MTILCHINDIEDDSAQSFDVDDRNIFVVKKFGKVFVYLNSCPHI
                     GIPLEFLPNDFLDNDKRYILCSNHGALFEIENGDCIAGPCVGQPLDAVPFTVEDGSIR
                     LL"
     gene            2081..3538
                     /locus_tag="VFX11_16180"
     CDS             2081..3538
                     /locus_tag="VFX11_16180"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4940217.1"
                     /translation="MFPFLSRCKCGLLLAVTALLGACANLYAPSNAPIDAIDNTRGYR
                     GMEKLASLGDVALVVSFSGGGTRAAALSYGVMQELRDTKLNAGGRQQNLLDEVDHISS
                     VSGGSFTAAYYGLFGYELFQHYEDDFLRQSVQGKLIKRMINPVNWFNSAFSGLDRTEM
                     AIDYYDSEFFRGKTFADMRPYPYIEINATDLSQGNRFAFTQLYFDLICSDLNSVKVAS
                     AVTASSAVPVLFPSVVLKNHAGHCDPSASLLWPQLQRTEFSSVRDRILVNSLKSYLNS
                     GERPYIHLVDGGISDNLGLRATIERLSLFGLNTSRRDSRLPRHVVVLEVNAQVQPRRP
                     IDSRAEKPSVADTVDAVSSAQIDRYTAETEALMETVMAEVQARAERDQLDTRFHLVKV
                     NFEAVAQDQFRRFFNNLPTSLELPNEEITTLIWAGRYLLRESPEFQAFLASPGLGGVP
                     ARAILHKKSRCQSKSRLQCLFQETGVGREQESPKD"
     gene            3577..4635
                     /locus_tag="VFX11_16185"
     CDS             3577..4635
                     /locus_tag="VFX11_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4940218.1"
                     /translation="MVPMLRSHPLLLLLILLALLLVALAASLSLGAVNVPLAESLGVI
                     VRHLAGAPAEASTADLVIWQIRLPRVLLSVLVGAALAVSGALVQGLFRNPLADPSLIG
                     VSGGAAVGAAIAIVLGGVVEGALGLGNAQWLLPLLAFAGGGAVTALVYRLGVSPLGTS
                     VATMLLAGVAINAMTGACISYLSTVAEDARLRNLVFWLMGSFNAASWPKVLWVAPFTL
                     LILVMIPFVWRGLNALLLGESEARHLGFSVERLRWRVILVTALAVGAAVAAVGVIGFV
                     GLVVPHIVRLLLGPDHRWLLPCCAVLGAALLCAADLAARMVVQPQELAIGVLTAFLGA
                     PFFLFLIVQRRRGQIHGA"
     gene            4625..5398
                     /locus_tag="VFX11_16190"
     CDS             4625..5398
                     /locus_tag="VFX11_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009836024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEX4940219.1"
                     /translation="MALDVRNLQFHIGQHSLLKALDLQVKAGECVAVVGPNGAGKTTL
                     FRLLAGEVEPSAGTIQFNGRELTRWPVAQLARMRAVLPQSSTLQFSFTVQEVVAMGRM
                     PHATGRKIDDGIVHDVMQVCDVRKLAERAYTALSGGERQRVQLARVLAQVWQPVADGE
                     RLLLLDEPTSALDIAHQIELMQVVRQVCAADIAVLVILHDLNLAARFANRILMLHEGS
                     LIAAGTPEQVLQPDLIECVFNVKTQVICHPLYGCPLVVS"
     gene            5626..>6141
                     /locus_tag="VFX11_16195"
     CDS             5626..>6141
                     /locus_tag="VFX11_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940220.1"
                     /translation="MFSTRINSILLAEADQHAQRRLCGVLQRLAPQASVQVTTSVAET
                     CEVLTQTPPVLAVINFTLIDGNFIDALKSIPRLPLPPFLLGLTRCPDCAETVYAMRYG
                     FNHVLQTAANAEQLDEVQFAHVLTQMVSEPAPLEATIGKLVNPLLYNQMAQFDLKQVS
                     GAIEQHIFSLAS"
BASE COUNT         1122 a         1713 c         1969 g         1337 t
ORIGIN      
        1 cagacgcctt acgccaccaa gcacgcattc aacggttggg tggaccagtt cctggtgacg
       61 ccggaccagg gtctgaatga caaatatgtc tcgctcggtt tcgacctgaa tgcctggacc
      121 accaagattc tgatccagta tcacgaatac gaatctgacg tggaatccgg tccgctgggc
      181 aaggaactgg atatgggcga agaaagcggt gtgcagattc tcaaaaccct gggccccaac
      241 tggacgctcg gcgccaaata cggcgactac aagcagccca gtcaggagtt caccaccggc
      301 aacacccgtc gcaacgccga caaagtgtgg ctgtgggctg agctgaattt ctgaaactcc
      361 gcgccatttt cacgcgcaac aaaaagggcc ctgcaaacgg gcccttttta ctgctctcct
      421 gcaatctgtc aggcaggttg ctcgcatccg gcacgatcaa cacctcgcct ccaccacccg
      481 cacataactt tcaccctcgc tacgcggtat atccagggcg tcataaaaat cttcaggtct
      541 gtgcacggtc acggactgga aaccgcagcg gcgaaagccg cgctccacat cagcgtcgct
      601 gcggaaatgc agattggtgc gcgactgcgt catcacgccc agtgctttct gcgccgccga
      661 caccagtaca cgtctgtgga attccttcac ttccggcacc agatcggtaa tgtaccaggc
      721 tgcagggaat tgccgtccca gttgcgccag tttttgccag aaacccgaga tggtcgccag
      781 atcgaaataa ttcaccagtc cttcagtgat gacgatggtg gggcggcccg gctccagcac
      841 gttggccagc acatgatcca gcccgttcgg ttcgccactt tcaaaaatgt tgcaggccac
      901 tactttatga ttcgggccga aaccctggtg gcgcagcagc agcgattgct tgcgagttgc
      961 catgccgggc agatccgctt ccacataatg cagatgcggg tactggcggg aaaaggtgta
     1021 gccccggggc gacaggccgc aggcgatttc cagaatctgg gtgacacctt cctgttcgat
     1081 cagctggcgg acgcggtgat ccataatgcg gtgccgctgc agcagcatgt cgctcaggct
     1141 catgcccagc aactgttcgc cgacgaaatt gatcgggcgt acggcgttgt acatcaggtc
     1201 ggcccggggt gaagtgaaaa agcgtgcaga catgccgtgc ttgtaccaga catgaccggt
     1261 ataaagtgcg gtgaagctga tgctggatgt atccatgttt ccccctgaaa tgagtaacgg
     1321 gcagggacgt ggccatgcct gaataatagt tcccggttgg ggttacagca ggcggatgct
     1381 gccgtcttcc accgtgaagg gcacggcatc cagcggctgg ccgacgcagg ggcctgcgat
     1441 gcagtcgccg ttttcgattt cgaacagcgc accgtggttg gagcagagga tgtagcgttt
     1501 gtcgttgtcg agaaaatcgt tgggcaggaa ttccagcggg atgccgatgt gggggcagga
     1561 gttcaggtag acgaacacct tgccgaattt tttcactacg aagatgttgc ggtcgtcgac
     1621 atcgaaactc tgcgcgctgt cgtcttcgat atcgttgatg tgacaaagga tggtcatgga
     1681 aagggcccta ctactgatcg atgagcgagt gcgggttcaa gaatagcctt tttcagatca
     1741 caaagtaaac gatgtgtgta ctggcggctg caggggcgtc ccgataaaaa attcgcggcg
     1801 gggattgttt tcgcgaatga taatgattag cattggcttc gtgtggtgag tgcaatgatc
     1861 cggtcgcccg aagcggtgat cgtcatggcc cctctctcct ctctacaacc ttgtcctcgt
     1921 cgcgcgggtt gaccgaatcg cccagtgcgc tcaccacaca ctcagtcaaa acatggtgca
     1981 ttgaccgggg aatgtccccg gtcttttttc ccgctttttt gagccgaacg ccgcgctcga
     2041 cttatcatac ccagttcttg ttcatcctcc tggagttgcg atgtttccgt tcctgtcccg
     2101 ttgcaagtgc ggcctgctgc ttgctgtgac ggcattgctg ggtgcctgcg ccaatctgta
     2161 tgcgccgtcc aacgcgccga ttgacgcgat cgacaacacc cggggctacc ggggcatgga
     2221 gaagctggca tcattgggtg atgtagcgct ggtggtgtcg ttttccggcg gtggcacgcg
     2281 ggcggcggcg ctgtcgtatg gcgtgatgca ggaattgcgc gacaccaagc tgaatgccgg
     2341 tggccggcaa cagaacctgc tggatgaggt ggaccacatc agttcggtgt ccggtggcag
     2401 cttcaccgct gcctattacg gcctgttcgg ttatgagctg tttcaacact acgaagacga
     2461 tttcctgcgc cagagcgtgc agggcaagct gatcaaaagg atgatcaacc cggtcaactg
     2521 gttcaacagc gctttttccg gcctggaccg cacggaaatg gccatcgatt attacgacag
     2581 cgaatttttt cgcggcaaga cctttgccga catgcggccc tatccctata tcgaaatcaa
     2641 tgccacggac ctgagccagg gcaaccggtt tgcgttcacc cagttgtatt tcgatctgat
     2701 ctgttccgat ctgaattccg tgaaagtggc cagcgccgtg acggcatcgt cggcagtgcc
     2761 tgtgctgttt cccagtgtgg tgctgaagaa ccatgccggc cattgcgatc ccagtgccag
     2821 cctgctgtgg ccccaattgc agcggaccga gttttccagt gtgcgtgacc gcattctggt
     2881 gaattccctg aagagttatc tgaacagcgg ggagcggccg tatatccatc tggtggacgg
     2941 cggcatttcc gacaacctgg gtttgcgggc caccatcgag cggctgtcgc tgttcgggct
     3001 gaatacgagc cgccgcgaca gccggttgcc ccgtcacgtg gtggtgctgg aagtgaatgc
     3061 gcaggtgcag ccaaggcgac ccatcgattc ccgggcggaa aagccgtcgg tggcggatac
     3121 ggtggacgca gtgtcgtcgg cgcagatcga ccgttacacg gcagaaaccg aggcgttgat
     3181 ggaaacggtg atggccgaag tgcaggcccg ggccgagcgg gatcagctgg atacgcgctt
     3241 tcatctggtg aaggtgaatt tcgaggcggt ggcgcaggac cagttccgcc gcttcttcaa
     3301 caatctgccc accagcctgg agttgccaaa tgaggagatc accacgctga tctgggccgg
     3361 gcgctacctg ctgcgggaat cgccggagtt ccaggccttt ctggccagcc cgggcctggg
     3421 tggtgtgccg gcgcgggcca ttctgcacaa gaagagtcgc tgccagtcga agtcgcggtt
     3481 gcagtgcctg ttccaggaaa ccggcgtcgg gcgggagcag gagagcccga aggactagtc
     3541 cggctgatat aatgccggca ctttgtcgtt cctgcgctgg tgcccatgct gcgttcccat
     3601 cccctgttgt tgttgctgat cctgcttgcg ctgctgctgg ttgcgctggc ggccagcctg
     3661 tccctgggcg cggtgaatgt gccgctggcg gaatccctgg gcgtgatcgt gcggcatctg
     3721 gcgggcgcgc cggctgaagc gtccacggcg gatctggtga tctggcagat ccgtctgccc
     3781 cgggtgttgc tgagcgtgct ggtgggcgcg gcactggcgg tgtcgggcgc gctggtgcag
     3841 ggactgttcc gcaatccgct ggcggacccc agcctgatag gcgtgtctgg tggtgcggcg
     3901 gtgggggcgg cgattgcgat tgtactgggt ggtgttgtgg agggggcgct gggtctgggc
     3961 aatgcacagt ggctgctgcc attgctggcg ttcgccggtg gtggcgcggt gacggcattg
     4021 gtgtatcgcc tgggcgtgag cccgctgggc acctcggtgg ccaccatgct gctggcgggt
     4081 gtggcgatca atgcgatgac cggtgcttgc atcagttacc tgtccaccgt ggccgaagac
     4141 gcgcgcctgc gtaatctggt gttctggctg atgggcagtt tcaatgccgc gtcctggccc
     4201 aaggtgttgt gggtggcgcc gtttacgctg ctgattctgg tgatgattcc ttttgtctgg
     4261 cgcgggctga atgcgttgct gctgggggaa tcggaagcac ggcatctggg attttcggtg
     4321 gagcgattgc gctggcgcgt gatcctggtg acggcgctgg cggtgggcgc cgcagtggcg
     4381 gcggtgggcg tgatcggttt cgtggggctg gtggtgccgc atatcgtgcg gttgttgctg
     4441 gggccggatc atcgctggct gttgccctgt tgcgccgtgc tgggtgccgc gttgctgtgt
     4501 gcggcggatc tggcggcacg catggtggtg cagccgcagg aactggccat cggtgtgctc
     4561 actgcatttc tgggcgcacc gtttttcctt tttctgattg tgcagcggcg gcgggggcag
     4621 attcatggcg cttgatgtcc gcaatctgca attccatatc ggccagcact cgctgttgaa
     4681 agcgctcgat ctgcaggtga aggcgggcga atgtgtcgcc gtggtcggcc ccaatggcgc
     4741 gggcaagacc acgctattcc gtctgttggc aggtgaagtg gaacccagtg ccggcaccat
     4801 tcaattcaat ggccgcgagc tgacgcgctg gccggtggcg caactggccc gcatgcgtgc
     4861 cgtgctgccg cagtcttcca cactgcagtt ttctttcacc gtgcaggaag tggtggcgat
     4921 ggggcggatg ccgcacgcga ccggacgcaa aatcgatgat ggcatcgtgc acgacgtcat
     4981 gcaggtgtgc gatgtgcgca aactggcgga gcgcgcgtac acggcgctgt ccggtggcga
     5041 gcgtcagcgc gtgcaactgg cgcgggtgct ggcgcaggtg tggcagccgg tggccgatgg
     5101 cgaacgtctg ctgctgctgg atgagcccac gtcggcgctc gacattgccc atcagatcga
     5161 actgatgcag gtggtgcggc aggtatgcgc ggcggacatc gcggtgctgg tgattctgca
     5221 cgacctgaat ctggcggcac gctttgccaa tcgcatcctg atgttgcacg agggcagcct
     5281 gattgccgcc ggcacgccgg aacaggtgtt gcagccggat ctgatcgaat gtgtgttcaa
     5341 tgtgaaaacc caggtaatct gtcacccgtt gtatggttgt ccgttggtgg tgagttgagc
     5401 gggcgatcac cgcccccgtc aacaaactcg cactactttt cgtacaacac ccggctcccg
     5461 gtttccgata ttcactgtct gcaatattgc ccacgccagt tggcacaatc accgctctgc
     5521 gcaattgcct ttcaggtgga ccacgcctaa tgtgaagggc gtcgtgaatc tatcgacacc
     5581 agcgcaacaa tggcggcgcg cgattgccgc aggatgaaac acggaatgtt cagcaccaga
     5641 atcaatagca tattgttggc ggaggcagat cagcacgcgc agcgccgtct gtgcggggtg
     5701 ttgcaacggc tggcacccca ggccagcgtg caggtcacga ccagtgtcgc cgaaacctgc
     5761 gaagtcttga cgcaaacgcc ccctgtgctc gccgtgatca atttcacgct gattgacggc
     5821 aatttcatcg acgcactgaa atccatcccg cgcctgcccc tgccgccatt tctgctgggt
     5881 ctgacccgct gccccgactg tgccgaaacc gtctatgcca tgcgctatgg cttcaatcat
     5941 gtgctgcaga ctgcggccaa tgccgagcag ctggatgaag tgcagtttgc gcatgtgttg
     6001 acgcagatgg tgtcggaacc ggcgccgctg gaggcaacca tcggcaagct ggtgaatccg
     6061 ctgctgtata accagatggc gcagttcgat ttaaaacagg tgagtggtgc catcgaacag
     6121 cacattttca gtctcgccag c
//
LOCUS       DASUEG010000859         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_342695, whole genome shotgun sequence.
ACCESSION   DASUEG010000859 DASUEG010000000
VERSION     DASUEG010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342695"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFX11_16200"
     CDS             complement(<1..122)
                     /locus_tag="VFX11_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4940221.1"
                     /translation="MEFAPILTIAAKEFRDRIRNRWVLAVSLIFTVFALVITYFG"
     gene            complement(133..1065)
                     /locus_tag="VFX11_16205"
     CDS             complement(133..1065)
                     /locus_tag="VFX11_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4940222.1"
                     /translation="MASTTPVSADTVIAVHGVTKHYGSVHAVDGVSLDCRRGELFGLI
                     GHNGAGKSTLFKMMLGLIPVTAGSIQIAGENVTGKGFRNVRRKIGYLPENIVLYDNLT
                     GLETLQFFARLKGLPQDHCAATLERVGLQHAADRRVNGYSKGMRQRLGFAQALLGNPQ
                     LLFLDEPTNGLDPEAIRGFYAIINDLKLQGVTIILTSHILAEIQERVDRLAILASGKL
                     QASGTVQQLREQMSLPLTITVKPAADAMASVRRILEQLPGLTVGTSADCITVHCPREQ
                     KMQVLTTVTALGPQVQDLHVREASLEDVFFGFAG"
     gene            complement(1037..2293)
                     /locus_tag="VFX11_16210"
     CDS             complement(1037..2293)
                     /locus_tag="VFX11_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX4940223.1"
                     /translation="MVLAVISAHPVYATTSTTWQIAPGDPIQPIINQATAGDVIELQR
                     GFYRENLRIEKPLTLRGLNRPTLSGGLQGDTVRITAQQVTIEGLIVRDSGDSLREQNA
                     GIYLQPGAHHVTVRNCDLTYNLFGIWIENADDVVIDNNIITGKRDYRSSQRGNGIQLY
                     NSQRARITNNNISFVRDGIYIDVSHHAVFSGNRLHHSRYGSHYMNSYYNLWEGNDSWM
                     NRGGLALMEVRDQVVRNNRAWANSDHGIMLRTIQNARVENNIVAGNARGFFIYDAEYN
                     TLRDNLVVDNVVGVHLWAGSKNNRVEGNDFISNREPVRYVGAKDEAWGTERGNHWSNY
                     LGWDRNGDNRGDIQYEANDIVDRLTWKHPMMKLLLASPAVQSLRLVGQQFPVLRAPSV
                     IDPNPQMRPSHPDWRDWLGKHYPGFR"
     gene            complement(2359..>2597)
                     /locus_tag="VFX11_16215"
     CDS             complement(2359..>2597)
                     /locus_tag="VFX11_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription regulator"
                     /protein_id="HEX4940224.1"
                     /translation="GRLHTGLAIGLWTAWCLFEVLERLGSKPYVKEGPWWQRNYRKAS
                     AMDLVSYVGFKNLVIGALLFLLLQSLDLLMLSAV"
BASE COUNT          497 a          735 c          799 g          566 t
ORIGIN      
        1 ccaaaataag taatgaccag tgcaaacacg gtgaagatca gactgacggc cagcacccag
       61 cgattgcgga tgcggtcgcg gaattccttg gcagcgatgg tgagaatggg cgcaaattcc
      121 atgcagcagt ccttaaccgg cgaatccgaa aaatacatct tccagcgacg cttcccgcac
      181 atgcagatcc tgcacctgtg gcccgagcgc ggtgacggtg gtcagtacct gcattttctg
      241 ttcgcggggg cagtgtacgg tgatgcagtc tgcactggtg ccaaccgtca ggccgggaag
      301 ttgttccagt attctgcgca cggatgccat ggcatcggca gccggtttca ccgtgatggt
      361 gagcggcaga ctcatctgct cgcgcagttg ttgcacggtg ccgctggcct gcaatttgcc
      421 ggaggccaga atggcgagac gatctaccct ttcctggatt tccgcgagaa tatgcgaggt
      481 gagaatgatg gtgacaccct gcagtttcag atcgttgatg atggcgtaga agccgcggat
      541 ggcttccgga tcgagtccgt tggtgggttc gtccagaaaa agcaattgcg gattgcccag
      601 cagcgcctgc gcgaaaccca gccgttggcg catgcctttg gagtagccgt tcacccgacg
      661 gtcagcggcg tgctgcaaac ccacccgttc cagcgtggcg gcgcaatggt cctgcggcaa
      721 tcctttcagg cgggcaaaaa actgcaaggt ttccaggccg gtgagattgt cgtacagcac
      781 gatgttttcc ggcagatagc cgatcttgcg tcgtacgttg cggaagccct tgccagtgac
      841 attttcaccc gcaatctgaa tgctgccggc ggtaacggga atcagcccca gcatcatctt
      901 gaacaaggtg cttttgccgg cgccattgtg gccgatcagc ccgaacagtt cgccgcgccg
      961 gcagtccagc gatacgccat cgacggcatg cacgctgccg taatgtttgg tgacgccgtg
     1021 cactgcaatc acggtgtcag cggaaaccgg ggtagtgctt gccaagccag tctctccagt
     1081 cagggtgcga aggtcgcatt tggggattgg ggtcgattac gctgggtgcg cgcagcaccg
     1141 gaaattgctg gcccaccagc cgcagcgatt gtactgcagg gctcgccagc agcaatttca
     1201 tcatgggatg tttccaggtc aggcggtcga cgatgtcgtt cgcttcgtat tgaatgtcgc
     1261 cccgattgtc gccgttgcga tcccagccca gatagttgct ccagtggttg ccgcgttctg
     1321 ttccccacgc ctcgtctttc gcgcccacgt aacgcaccgg ttcgcggttg gaaatgaaat
     1381 cgttgccttc caccctgttg ttcttggagc cggcccacag gtgcacgccg accacattgt
     1441 ccaccaccag gttgtcccgc agcgtgttgt attcggcatc ataaatgaaa aagccgcgcg
     1501 cgttgccggc gacgatattg ttttccaccc gcgcgttctg gatcgtgcgc agcatgatgc
     1561 cgtgatcgga gttggcccag gcgcggttgt tgcgcactac ctgatcgcgc acttccatca
     1621 gggccaggcc gccgcgattc atccagctgt cgttgccttc ccacaggttg taataggaat
     1681 tcatgtagtg ggaaccgtag cggctgtgat gcaggcgatt gccgctgaaa accgcgtggt
     1741 gcgatacatc gatatagata ccgtcgcgca cgaagctgat gttgttgttg gtgatgcggg
     1801 cccgctgcga gttgtacagc tggatgccgt tgccgcgctg ggaagagcga tagtcgcgct
     1861 tgccggtgat gatgttgttg tcgatgacca catcgtcggc gttttcgatc cagatgccga
     1921 acaggttgta ggtgagatcg cagttgcgca cggtgacgtg gtgtgcgccc ggttgcaggt
     1981 agatgccggc attctgttcg cgcaggctgt cgccggaatc gcgcacgatg agaccttcaa
     2041 tcgtgacctg ctgtgccgtg atgcgcacgg tgtcaccctg cagtccgccg gacagggtgg
     2101 ggcggttgag gccacgcagg gtgagtggtt tttcgatgcg caggttttca cgataaaagc
     2161 cccgctgcag ttcaatgacg tcgccggcag tggcctgatt gatgatgggt tggatggggt
     2221 cgccgggtgc gatctgccag gttgtggatg tggttgcgta aacgggatgg gcactgatca
     2281 ctgccagcac catcagcagc gccagtatcg ttccggtggg ttttatggtc cggccgcgcc
     2341 accaccgtct cattgtcatc aaaccgcgct cagcatcagc agatcgagcg actgcaacag
     2401 cagaaacagc agcgcaccga tgaccagatt cttgaaaccc acgtagctga ccagatccat
     2461 cgcgctggcc ttgcggtaat tccgttgcca ccagggacct tccttcacat agggctttga
     2521 gcccagccgt tccagcacct cgaacagaca ccaggcggtc cacaggccga tggcaaggcc
     2581 cgtgtgcaag cggccat
//
LOCUS       DASUEG010000860         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_485389, whole genome shotgun sequence.
ACCESSION   DASUEG010000860 DASUEG010000000
VERSION     DASUEG010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485389"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="VFX11_16220"
     CDS             <1..104
                     /locus_tag="VFX11_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4940225.1"
                     /translation="TGALSWSNYWYKPDGPLTIDQLAEQALALALKI"
     gene            464..1147
                     /locus_tag="VFX11_16225"
     CDS             464..1147
                     /locus_tag="VFX11_16225"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4940226.1"
                     /translation="MSASESSVMEFGGVQERGPDARDRILRAAESLFAIRGFEGVSTT
                     QIAKVAGITQPLIHYHFKNKEALWKATVARLFSRMSEEFSAELRGLPQTDSRRYMVEM
                     IRSYVTFVARYPQFGQFILREGVQQSSRLAWMVDEWLKPLLNQFQDVYKKGLSEGWVK
                     DIPFPQLIMLITASASHFFSLAPLVNSLYGIDVNSPDQVLAHSDAVVEVAVNAILRAP
                     VGAEPALSA"
     gene            1211..1453
                     /locus_tag="VFX11_16230"
     CDS             1211..1453
                     /locus_tag="VFX11_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940227.1"
                     /translation="MLAPVLAWRWGRGVIGNGFPFGSTGYVILEEGDLDPASFQLQVR
                     HYLVVKPSGETVSGIFTLPEARDFILQAESKILKNE"
     gene            1581..1656
                     /locus_tag="VFX11_16235"
     tRNA            1581..1656
                     /locus_tag="VFX11_16235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1614..1616,aa:Val,seq:tac)
     gene            1663..1739
                     /locus_tag="VFX11_16240"
     tRNA            1663..1739
                     /locus_tag="VFX11_16240"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1697..1699,aa:Asp,seq:gtc)
     gene            complement(1986..3137)
                     /locus_tag="VFX11_16245"
     CDS             complement(1986..3137)
                     /locus_tag="VFX11_16245"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX4940228.1"
                     /translation="MFAQANTIGIIGAKGLLFIAALLVALFTSFNSHAAALTVVSQPS
                     SKTITEGSSISFTVKATSSRTIRYAWFKNGIQLNDKNSRTLTISKATPSNAGTFYCKV
                     TDGVTTIRCKSFTLSVNKSGALSITRQPADTVIYEGKSTTFSVATNSTKPLTYAWYKD
                     GSQLSNKTASTLTISSATPGNAGTFSCKVSDGVTTVSCASFKLTVNKIVRITQQPVNQ
                     IQSAGTSASLSVTATGTAPLSYQWYYNGTAISGATASTLKFASATVNNSGKYYCVVKN
                     PGSSATSTTASLSISSAAKTGSVKISWARPTTRADGTALAATSIAGYDLYHSSSSASS
                     MTRLSSLTAAELSIVVTDLSLGTHYFALATKDASGMQSKLSSTITVSIQ"
     gene            complement(3605..4630)
                     /locus_tag="VFX11_16250"
     CDS             complement(3605..4630)
                     /locus_tag="VFX11_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4940229.1"
                     /translation="MKIGDTTASYISYLLECADSLGASVDRLLADAELNPRELQDGDN
                     RIDLTYLMRLGYGAIRQTGRPDIGLILGRQTGITRFGHAGLAAMTAPTLGDALEIITR
                     YEPLHGRCYRGSSQLTRNGQQTQLEFYSIAPYNDYTYFVVDAVLGGWYQIIQWMTGRT
                     DLVAHALIEFSTPAYYQAYDATFPCGVRFHQELNGLVLKPGALATPLLHYNPALHASL
                     LRTCDELLTQVAMADSYRNKVLKVLGTMLHGKTPSIEEVAQQLCIPPWTLRRKLKDED
                     TAYQNLVDEMRRDVALSYMKNTDLSFGEIAYLLGFSTPGAFQRAFKRWAGTTPGEYRK
                     SLEMQRK"
     gene            4899..6302
                     /locus_tag="VFX11_16255"
     CDS             4899..6302
                     /locus_tag="VFX11_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940230.1"
                     /translation="MAMLDSSGSNEAVLFFEEGQVSKEMTWSEFEAILDGVVGINDFA
                     GEKRLAAFVTVTGRLKVTGVALFTITFDKQGFADKSWNLPLRHLVDTGGAGPDLGAGP
                     IKLACKSQCSVAWHAGMLWDPDLSPRTNTFVRIRDAIADNKLHLPVHAEVEVAPVSVP
                     QRAPGNWGMAPPPPTLNSPWDSDSGLTEAQLAALESQHRNKIAALIKQQRLHIQTLTT
                     ENQHNISSTKLHYEKELQLAKIEIARLRSEHESLHSQNIALREQNEAQRKQLETLKKS
                     LEQEMYKAQKNDQAEIDALRAHYEQLMPQRIQEETAKLKEDIELRNMELLYRHEVAKQ
                     LREELCQLRKDKIRLVNEGGDKFLERLEALGISFIAFHPGAGHVSIQLGEMASYMENP
                     IAYAADKCLVSEDHYRHWLAHYQKPECQAPLTNETICGCRIRRVDVPSQFLMGESDRC
                     DKHKPRSFGDNVVNLRG"
     gene            6374..>6570
                     /locus_tag="VFX11_16260"
     CDS             6374..>6570
                     /locus_tag="VFX11_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940231.1"
                     /translation="MSAVPSLNQIADYEQSLQQWPELVASGLLVQPAIESLSLPLFAQ
                     RDVRVDVLRADLLHPRISGNK"
BASE COUNT         1341 a         1706 c         1986 g         1537 t
ORIGIN      
        1 tgacgggtgc gctcagctgg tcgaactact ggtacaagcc cgacggtcct ctcaccatcg
       61 atcaactggc agaacaggct ctggcccttg cgctgaaaat ctgaaaacga gcgctgtccg
      121 atcaagcctg acctaccgat aaaaatggcc attttatgcg tcgatcaggt cgctggatgg
      181 ccacaaaata caagctcttc cagcgcgtga atttgggtaa cttaagcccg tgatttcatt
      241 cacaaagcgg atacgaaaat tacaattctc ttgattgagt agacgtttat aaagctgcta
      301 gtatccttga acgttaattg ttctatcgca gtacaacgtc gcaatttgcg tgttttcctt
      361 ggcatttctg acggtattgg tgcgcatccg cgtgctcgtc aaagctgcat ctggggtgga
      421 aaacagcaca tagtaaaaac aaaaaaaata aaaggtggaa agtatgagcg catcggaatc
      481 gtcagtgatg gaatttgggg gcgtgcagga gcgggggccc gacgcgcggg acagaatact
      541 tcgcgctgct gaatcgttgt ttgccattcg tggctttgaa ggggtttcca caacccagat
      601 cgccaaggtt gccggcatca cccaaccgtt gattcactat cacttcaaga acaaggaagc
      661 gctctggaaa gccacggtcg cgcgcctgtt cagtcgcatg tctgaagaat tttccgcaga
      721 attgcgaggt ctcccgcaaa ccgacagccg ccgttacatg gtggaaatga tccgctccta
      781 cgtcaccttc gttgcccggt atccccagtt tggccagttc atcctgcgcg agggcgtaca
      841 gcaatcctcc cgtttggcct ggatggtgga tgagtggctc aagcccctgt tgaaccagtt
      901 ccaagacgtt tacaaaaaag ggctttcgga aggttgggtg aaggacattc cattcccgca
      961 gctaatcatg ctgatcaccg cctctgccag ccatttcttc tccctggcac cgctggtgaa
     1021 ttccctttat ggcattgacg tgaacagccc tgaccaggtg ctggcgcaca gcgacgcggt
     1081 ggtggaagtg gccgtcaacg caatcctgcg ggcaccagtg ggagcggagc ctgcgttgtc
     1141 tgcctgagcg cgcacgctga ttgcaatttc cagtgactgc tgtaaaaagg gcgccctgcg
     1201 gggcgctttt ttgttggcgc ctgtcttggc ttggcggtgg gggcggggcg tgattggcaa
     1261 tggttttcct tttggcagca cgggctatgt gatcctggag gagggcgatc tggacccggc
     1321 cagtttccag ttgcaggtgc gtcattatct ggtcgtgaaa ccttccggtg aaactgtgtc
     1381 aggcatcttt actttgccgg aagcccggga cttcattctt caggctgaat caaaaatatt
     1441 gaaaaatgaa tgagttacat gatcgcctaa ataatgcagg atataggcga agtggttact
     1501 tacatgatga aattatttca gtttttagca tttagcgttt gacagccctg ccagaattcg
     1561 taaaatggcg cccacttctc gggtggttag ctcagctggg agagcatcgc ccttacaagg
     1621 cgagggtcac aggttcgatc cctgtaccac ccaccaaaat acggagcggt agttcagttg
     1681 gttagaatac cggcctgtca cgccgggggt cgcgggttcg agccccgtcc gctccgccaa
     1741 cattcagcca gtggttctgc attgcttgcc tggatagtga tactaaaaac ccctggatcg
     1801 caagattcag gggttttttg tttctgcgtc ggctaaaacg tcagagttct ggttccagct
     1861 tcatccccgc ttgaaatgca ttgatcccgc ctgtagcaga tgctgatgct cagagaagcg
     1921 gtgcccggca gaatccttgg cgtcaacacg aggaggtaga gcggttcgtg ttgacgccag
     1981 cctgcttact ggatgctgac ggtgatggtg gaggacagtt tgctctgcat gccgctggcg
     2041 tctttggtgg ccagggcgaa gtagtgggtg ccaagcgaaa ggtcggtcac cacaatgctc
     2101 agttccgctg cagtgaggct gctgagcctg gtcatgctgg aggcgctgct gctggaatgg
     2161 tacaggtcat aacccgcaat gctggtggcc gcgagcgcgg tgccatcggc acgggtggtc
     2221 ggtctggccc agctgatctt gaccgaaccg gtcttggccg cggagctgat gctcagcgaa
     2281 gcggtggtgg aggtggcgga tgagcccggg ttcttcacca cgcagtagta cttgccggaa
     2341 ttgttgacgg tcgcgctggc gaatttcagt gtgctggctg ttgcaccact gatggcggta
     2401 ccgttgtagt accactggta gctgaggggt gccgttccgg ttgccgtgac tgacaggctc
     2461 gcgctggtgc ccgcactctg gatctggttg actggttgct gggtgatgcg cacgatcttg
     2521 ttcacggtca gcttgaatga cgcgcagctc acggttgtga cgccgtcgct gaccttgcag
     2581 gagaaggtgc cggcattgcc cggtgtggcg ctgctgatag tcagcgtgct ggcggttttg
     2641 ttgctgagct gggaaccatc cttgtaccag gcgtatgtca ggggcttggt gctgttggtg
     2701 gccacgctga atgtggtcga cttgccttca tagatgacgg tgtccgccgg ttgcctggtg
     2761 atgctcagcg cgcctgactt gttgacgctc agagtgaatg acttgcagcg gatggtggtg
     2821 acgccgtcgg tcaccttgca atagaaagta ccggcgttgc tcggggtggc tttgctgatg
     2881 gtcagggtgc ggctgttctt gtcgttcaac tgaatgccgt tcttgaacca ggcgtagcgg
     2941 atggtgcggg aactggttgc cttgacggta aaggaaatcg agctgccttc tgtaatggtt
     3001 ttgctggacg gttgcgacac tacggtcaat gcggcggcgt gggaattgaa gctggtgaac
     3061 agggcaacaa gcagggccgc aatgaacaac aaacccttcg caccgatgat cccaattgta
     3121 ttggcttgag caaacatcca tttcaccttt attggctggg actaattcgt ttcgacgtgt
     3181 ggattctagg tgcaggtagt tgctgtcaag tgcggcacgg ttcacaaact gataggccac
     3241 acaaagtctt gtcgccattg ttgtggacaa agtcgcattt ttttcgtgaa tttctgtctg
     3301 gcaccgactt gatgcgctgg cgggatttgg tgtattagcc tgtttttcga gcagtaaacg
     3361 ggagcttcac cggtcacaaa agcttaacct gccgttcacc cgcttttaat tttcccggtt
     3421 tttaataggc actgtggaat ggcatcccgg ccggtgtttt tggcatgatg cgtgaaagga
     3481 tttcaaagcc aatagaaaac aggagaacgc tcatggatca ctatcaggat gaattgctgg
     3541 aacgtcgtga aaacgagttt gatgacgaag gcgacgacgc ctacgaaatg tagaacttgc
     3601 cagcttattt tcgctgcatt tccagcgatt tacggtattc ccccggcgtt gtgccggccc
     3661 agcgcttgaa ggcccgctga aacgcgccgg gggttgaaaa tcccagcagg taggctattt
     3721 caccgaacga cagatcggtg tttttcatat agctgagcgc tacgtccctt cgcatttcgt
     3781 ccaccagatt ctggtaggcc gtgtcctcat ccttcagttt gcgacgcagg gtccagggtg
     3841 gtatgcacaa ctgttgtgca acctcttcga tggacggtgt cttgccgtgc agcatggtgc
     3901 cgagcacttt caataccttg ttgcggtagc tgtcggccat ggccacctgg gtcagcagtt
     3961 cgtcgcaggt ccgcagcagg ctggcatgca gggccggatt gtagtgcagc agcggggtgg
     4021 cgagtgcgcc gggcttcagc accagtccat tgagttcctg atgaaagcgc acgccgcagg
     4081 ggaaagtggc gtcgtaggcc tggtagtagg ctggcgtgga gaattcgatc agtgcatggg
     4141 ccaccaggtc ggtgcggccg gtcatccact ggataatctg gtaccagccc cccaggacgg
     4201 cgtccacgac gaaataggtg taatcgttgt agggggcgat ggagtagaac tcgagctggg
     4261 tctgctgtcc gttgcgggtc agttgtgatg atccgcgata gcagcggcca tggagcggct
     4321 cgtagcgggt gatgatttca agggcatcgc caagagtcgg ggcggtcatg gcggccagcc
     4381 cggcgtggcc aaagcgggtg atgccggtct ggcggcccag gatcaggcca atgtcgggtc
     4441 ggccggtctg gcggatggcg ccgtagccca gtcgcatcag gtaggtgaga tcgatgcggt
     4501 tgtcgccatc ctgcagttcg cgggggttga gttccgcatc ggccagcagg cggtccaccg
     4561 atgcgcccag gctgtcggca cattccagca ggtaggaaat gtaactggcg gtggtgtctc
     4621 cgattttcat tcagattcga ctgcaggtgg ttgatcggca atggttcaca ttatataggc
     4681 aagcaccgga attgggattg gcgaaaagac tggttgtttt ttgagcttgg catcacagtc
     4741 cgcctgtatg gccggtcact tcctctcacg atacaacgcc ttagtatctc tccatatctg
     4801 cgaaggctgg ctacactgat agtatcttgc actttttccc ggtatccgtg tggcgccact
     4861 gatggcgccg ccatttcggc aaaggatatt cacctgtaat ggccatgctc gattcatccg
     4921 gctccaacga ggcagtattg tttttcgagg aaggccaggt ctccaaggag atgacctggt
     4981 ctgaattcga agccattctc gatggcgttg ttggcatcaa tgattttgcc ggcgaaaagc
     5041 gtctggccgc cttcgttact gtgaccggcc ggctgaaagt cacgggcgtg gccctattca
     5101 ctataacgtt cgacaagcag ggttttgccg acaagtcctg gaatctgccc ttgcggcatc
     5161 tggtggatac cggtggagcc ggccccgatc tgggtgccgg ccccatcaag ctggcctgca
     5221 aaagccagtg ttcggtggcc tggcatgccg gcatgctctg ggaccctgat ctgtcgccgc
     5281 gcaccaatac cttcgtccgc attcgcgacg ccattgccga caacaagctg catcttcccg
     5341 tgcacgcgga agtggaggtg gcgccggtgt ccgtgccgca acgggctccc ggtaactggg
     5401 gcatggcccc gccgccgccc acgctgaatt cgccctggga cagtgattcc ggtctgacgg
     5461 aagcccagct ggccgcgctg gaatcgcagc atcgcaacaa gattgccgcc ctgatcaagc
     5521 agcagcggct gcatattcag acgctgacga ccgaaaacca gcacaatatt tcctccacca
     5581 agctgcatta cgaaaaggaa ctgcagctgg ccaagatcga gatcgcgcgg ctgcgcagcg
     5641 agcatgaatc cctccatagc cagaacattg ctctgcggga acagaacgag gcccagcgca
     5701 agcagctgga gaccctgaag aaatcccttg agcaggaaat gtataaggca caaaaaaacg
     5761 accaggcgga aatcgatgca ctgcgcgcgc attatgagca gctgatgccg cagcggatcc
     5821 aggaggaaac cgccaagctc aaggaagata tcgagttgcg taacatggag ctgctgtacc
     5881 ggcacgaagt ggcgaaacaa ttgcgggaag agctgtgcca gttgcgcaag gacaagatcc
     5941 gcctggtcaa cgaaggtggc gacaagttcc tggaacggct ggaagcgctg ggtatcagtt
     6001 tcatcgcgtt tcaccccgga gcgggccacg tgtcgatcca gctgggcgaa atggcgtcgt
     6061 atatggaaaa tccgattgcc tatgccgccg acaaatgtct ggtgtcggag gatcattacc
     6121 ggcactggct ggcccactac cagaagccgg aatgccaggc tcccctcacc aacgaaacga
     6181 tttgcggttg ccgcatccgt cgtgtggatg tgcccagcca gttcctcatg ggcgaatccg
     6241 atcgttgcga caagcacaaa ccccgttcgt tcggtgataa cgtcgttaat ctgcgcggct
     6301 gatgttggcg ctgctcccgg tggcgctaca atggttcttt tcccggtgag tacggttctg
     6361 tgagtgctgt cctgtgagtg ccgtcccgtc gctcaatcag attgccgatt acgaacaatc
     6421 cctgcagcag tggcctgaac tggttgccag cgggttgctg gtgcaacctg ctatcgaatc
     6481 cttgtcgttg cccctgtttg cgcagcggga tgtgcgcgtc gatgtgttgc gcgccgacct
     6541 gctgcatccg cgcatttccg gcaataaatg
//
LOCUS       DASUEG010000861         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_522749, whole genome shotgun sequence.
ACCESSION   DASUEG010000861 DASUEG010000000
VERSION     DASUEG010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522749"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFX11_16265"
     CDS             complement(<1..394)
                     /locus_tag="VFX11_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940232.1"
                     /translation="MKLKALAAAVALMSTATVANADWNDGKNAVSGAASGNGELVLLV
                     WDQVLEVSVVQDLGDSYLNFYDNSQNSAFTFDANLDALFASTFANSNAADIQWSVFAN
                     SAGSTLSSNPERYKNGFMVTVNDAEENPT"
     gene            1060..>2200
                     /locus_tag="VFX11_16270"
     CDS             1060..>2200
                     /locus_tag="VFX11_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4940233.1"
                     /translation="MASTFLLPYRRQIVYSLTALIFTASAMLSIGQGLRLLIDQGIAT
                     ASSDRLAHYVLVFMGLAVALAVGTFARHYWVTWLGERVVADIRAKVFNHLIDLHPGFF
                     EQNRALEIQSRLTADTTLLQSVVGSSISMALRNLILMAGGIVWLFITNVKLTVIVMIS
                     VPLVITPILFFGRRVRHLSSKSQDKVADVGAYVGEILGNIRTVQAYNHQPLDKQRFHG
                     IVEAAFDMAKRRIVQRSLLITAVIVLVLGAVSIMLWVGGQDVIAGRITGGELAAFVFY
                     AVIVGSAVASISEVIGELQRAAGAAQRIIELLQSTSTIQAPSQPAVLSQPVQGRIDIQ
                     SLTFAYPTRPDVFAIHDLTLNIRAGETLALVGPSGAGKSTLFDLLL"
BASE COUNT          477 a          638 c          607 g          478 t
ORIGIN      
        1 cagtggggtt ttcttctgcg tcgttcacag taaccatgaa gccgttcttg taacgctccg
       61 ggttggacga cagagtggaa ccggcgctgt tggcgaatac cgaccactgg atatcagccg
      121 cattggaatt ggcgaaagtg ctggcgaaca gtgcatccag gttggcatcg aaggtgaacg
      181 cgctgttctg gctgttgtca tagaaattca ggtaggaatc gcccagatcc tgaacaaccg
      241 acacttcaag aacctgatcc cataccagca gaaccagttc accgttaccg gaagcagcgc
      301 cggacactgc gtttttgccg tcgttccagt ctgcgttggc aacagtggca gtggacatca
      361 gggcaactgc ggcagcaagt gctttcaatt tcatctgtat ttcctctcga tgattaaaaa
      421 aatctgtaag actccgattg ggtgcggaaa cagaatttcc gcaggttttg aaaatcaagc
      481 tccgccgtca tccaccccaa aatcatttct ttgcgtgctg gaacctttcg cgagatcgaa
      541 tttcaaaacg aggccactat aaccaagcgc tggggtcagt cggtgaacgt ttcgtgaatg
      601 tttttttaag ttttcagcgc aaaaacaagt agttattcgg ggatgtgcag aaacaaacct
      661 gtcatatgat tcgcgcaaaa gttgcggatt gctgtaaccc gtctgcaaca ggacagacgg
      721 ccaaaagcgg ggacacaccg ctacccgggc aagccgcatc tgcattcaat ccttgaaaat
      781 caagatgatg accctgcggc agggccatca cccacccaac caggcaatcc aaaagtagaa
      841 aaatgcgatg tcagttcgat gccactctcg tggcagccgc atggcgttat caacaggctt
      901 atctacaggc tgccttgagg ggcccattgt catgaagcgc tcacccggag caggcacaaa
      961 gcagtggaca aatcaccgcc agcgggcaaa atgattttcc ggtcaatctc ccccaaggat
     1021 cacctccccg tgcacaccaa ccgatggcag gccacgcgca tggccagcac cttcctgctg
     1081 ccctaccggc gccagattgt ctattcactg acagccctga tctttaccgc atccgccatg
     1141 ctgtccatcg gccaggggtt gcgtctactc atcgatcagg gcattgccac tgcttcgtcc
     1201 gaccggctag cacactacgt actggtcttc atgggactcg cggtagcgct ggcggtgggt
     1261 accttcgccc ggcattactg ggtgacctgg ctgggggaac gggtggtggc agatatccgg
     1321 gccaaggtgt tcaaccacct gatcgatctg catcccggct tctttgagca gaatcgcgca
     1381 ttggaaatcc agtcgcgact caccgccgac accaccctgc tgcaatccgt ggtgggttcg
     1441 tccatttcca tggcgctgcg caatctgatc ctgatggcgg gcggcatcgt ctggctgttc
     1501 atcaccaacg tcaaactgac cgtgatcgtg atgatctccg tgccgctggt gatcacgccg
     1561 atcctgtttt tcgggcggcg ggtacgccat ctgtccagca agagccagga caaggtcgcc
     1621 gacgtgggtg cctatgtggg cgagattctc ggcaacatca gaaccgtgca ggcctacaac
     1681 caccagccgc tcgacaagca acgctttcac ggcatcgtgg aagccgcgtt cgacatggcg
     1741 aagcgacgca tcgtgcaacg aagcctgctg attaccgccg tcatcgtact ggtgctgggt
     1801 gcggtgagca tcatgttgtg ggtgggcgga caggatgtca ttgcgggacg catcaccgga
     1861 ggcgagctgg cggcattcgt attttatgcg gtcattgtcg gctcggcggt ggcctcgatt
     1921 tcggaagtga tcggcgaatt gcaacgggct gccggtgccg cgcagcgtat catcgagctg
     1981 ttgcaatcca cgtccactat ccaggcgcca tcacagcccg cagtgctgtc gcaacccgtg
     2041 cagggccgca tcgatatcca gtccctgaca tttgcctatc cgacgcggcc ggacgtattc
     2101 gccatccacg atctgaccct caatatccgg gccggagaaa cgctggccct ggtgggcccg
     2161 tcgggcgctg gcaaatccac cctgtttgat ctgctgctgc
//
LOCUS       DASUEG010000862         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_608243, whole genome shotgun sequence.
ACCESSION   DASUEG010000862 DASUEG010000000
VERSION     DASUEG010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608243"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1715
                     /gene="gltX"
                     /locus_tag="VFX11_16275"
     CDS             210..1715
                     /gene="gltX"
                     /locus_tag="VFX11_16275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX4940234.1"
                     /translation="MTVRTRVAPSPTGDPHVGTAYIALFNRVFAHQHGGQFILRIEDT
                     DQVRSTPESEAMILDSLRWMGLTWDEGPDVGGPHGPYRQSERSAIYREHADLLVQMGH
                     AFHCYRTPEELDALRQQKIESGNQTALKPSELALPADEVARRKAAGAPYVIRMMVPEE
                     GKCPIDDMLRGVIELDWAQVDAQVLMKSDGMPTYHLANVVDDHLMQITHVLRGEEWIN
                     SAPKHKLLYQYFGWEMPKLCHMPLLRNPDKSKLSKRKNPTSINYYRRMGFLPEALVNY
                     LGRMGWSMPDEREKFSLQEMQAHFDLMRVSLGGPVFDIAKLSWLNGLWIRESLTVEQL
                     AQKFQEWAFNESYLTQILPLIQKRLEVLSDVVKLADFFVAGQVELNAADLQTGKLEPD
                     QTRKILQYSLWRFEEQRSWNRDTISTALSALSTSMGIKLKDFMLPLFVAVSGRQSAPS
                     IMDAMAIIGSDMTRARLRHALDLLGGPSKKEAKEWEKEYRAFNQKDAPENC"
     gene            1823..1898
                     /locus_tag="VFX11_16280"
     tRNA            1823..1898
                     /locus_tag="VFX11_16280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1856..1858,aa:Ala,seq:ggc)
     gene            1917..1992
                     /locus_tag="VFX11_16285"
     tRNA            1917..1992
                     /locus_tag="VFX11_16285"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1951..1953,aa:Glu,seq:ttc)
BASE COUNT          474 a          725 c          573 g          412 t
ORIGIN      
        1 atgcaccagc gccattgcgg gggagaagcg cgtcgttatt tcaggcccct gacactcaag
       61 ctggcggtta ttcagactgg ctgacattga acaacgtcgt tctggcttcc tgcgcttcag
      121 gtggcggcaa cggacaaaac ccgttccccg ccccctgtcc atccgatata taattgccgc
      181 ctttcttttt ccggacagga ttctccccca tgacagtcag aacccgcgtc gccccgtccc
      241 cgaccggcga tccccacgtt ggcaccgcct acatcgccct gttcaaccgg gtgttcgccc
      301 accagcacgg cggccagttc atcctgcgga tcgaagacac cgaccaggta cgcagcaccc
      361 cggaatccga ggccatgatc ctcgactccc tgcgctggat gggcctgacc tgggacgaag
      421 gcccggacgt cggcggcccc cacggcccct atcgccagag cgagcgttcc gccatctacc
      481 gtgaacacgc cgacctgctg gtgcagatgg ggcacgcctt ccactgctac cgcaccccgg
      541 aagaactcga cgccctgcgc cagcagaaaa tcgaatccgg caaccagact gcgctaaagc
      601 cttccgaact ggcactgcct gccgacgaag tggcccgtcg caaggccgcc ggcgctccct
      661 acgtcatccg catgatggtg ccggaagagg gcaagtgccc catcgacgac atgctgcgcg
      721 gcgtgatcga actggactgg gctcaggtgg acgcacaggt actgatgaaa tccgacggca
      781 tgcccaccta ccacctggcc aacgtggtgg acgaccacct gatgcagatc acccacgtcc
      841 tgcgcggcga ggagtggatc aactccgccc ccaaacacaa attgctgtac cagtatttcg
      901 gctgggaaat gccgaaactc tgccacatgc ccctgctgcg caatcccgac aagagcaagc
      961 tcagcaagcg caagaatccc accagcatca actattaccg ccgcatgggc ttcctgcccg
     1021 aagcgctggt caactacctg ggccgcatgg gctggtccat gccggacgag cgggaaaaat
     1081 tctccctgca ggaaatgcag gcgcacttcg acctgatgcg cgtctccctc ggcggcccgg
     1141 tcttcgacat cgccaaactc agctggctca acggcctgtg gattcgcgaa tccctcacgg
     1201 ttgaacagtt ggcgcagaaa ttccaggaat gggccttcaa tgagtcctac ttgacccaga
     1261 tcctgcccct gatccagaag cggctggagg tgctgtcgga cgtggtgaaa ctggcggact
     1321 tcttcgtcgc tggccaggtg gaactgaacg ccgccgacct gcagaccggc aaactcgagc
     1381 ccgaccagac ccgcaagatc ctgcaataca gcctctggcg cttcgaggaa cagcgcagct
     1441 ggaaccgcga caccatcagc accgccctca gcgccctgtc caccagcatg ggcatcaagc
     1501 tgaaggactt catgctaccc ctgttcgtgg ctgtatctgg ccgccagtcc gccccttcaa
     1561 taatggacgc catggccata atcggctccg acatgacccg cgcacgcctg cgccatgccc
     1621 ttgatttgct tggcggaccc agtaagaagg aggccaagga atgggagaag gaatatcgag
     1681 catttaatca aaaggatgcc cctgaaaact gttgacagcc cctgggatag tccataaaat
     1741 tcgcaacctc ttgaggctga tcttcaagtt ttcaactggc aaaactctat tgaaaacaga
     1801 aggttggccc ttgatccaga ttggggctat agctcagctg ggagagcgct acaatggcat
     1861 tgtagaggtc ggcggttcga tcccgcctag ctccaccaat ctgaaacacc ctcagcgtcc
     1921 ccttcgtcta gcggcctagg acactgccct ttcacggcag taacaggggt tcgaaccccc
     1981 taggggacgc cacttttcct tcgttttacc taccattctt ttgcctcatt cctgtctgtc
     2041 cttcatgtca tttcaattca catggagcgc aacatggaag tcatcaagac cctcagacct
     2101 ggcgacagcg gtaccaagcg actcaccgcc cgttatggcg accggctaat ctgcgtccgt
     2161 tatcgcaagg acgaagcgct taac
//
LOCUS       DASUEG010000863         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2110115, whole genome shotgun sequence.
ACCESSION   DASUEG010000863 DASUEG010000000
VERSION     DASUEG010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110115"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..258)
                     /locus_tag="VFX11_16290"
     CDS             complement(19..258)
                     /locus_tag="VFX11_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940235.1"
                     /translation="MKTIYDTSVTFTFRGKPKTVKRVIKNQARSFLLSHRKKTEPIPF
                     FYPDFPDQPHYSKHRLLQRRPLLQTGRRGNYMTVA"
     gene            323..1438
                     /locus_tag="VFX11_16295"
     CDS             323..1438
                     /locus_tag="VFX11_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4940236.1"
                     /translation="MKVRLLPLAISAAIAMPGVALAEGPTVYGKLNVSYEMVDVDYAY
                     DDIDPNSADRWDLLSNASRFGVKGDAAINDSLKAIYQIEWEVGADEGNTFTSRDRFVG
                     LAGGFGTLQAGKFDSPLKKSQGKIDQFNDLAGDIKYVLLGEERLDNVIQYSTPVMGGF
                     QGNIAFAPGEEYDQGQDDPKDGPADSFSLSGTFTVDALYLALAYDSQVATEAYTGIDS
                     DFELTDTNDLIGEIADELDVDSETAYFDTVRLVAQYTMGDFQLGALYQMAETSDPLFT
                     VLDLEQDGFVVSGAFTLGQNVFKLQYGASTTEANVDEVDFSAENDATSLGLGMDHKLS
                     KQTTLFAYYNALSYEFDDEDAEEQTKDNLGVGIVHNF"
     gene            complement(1603..>2792)
                     /gene="fabB"
                     /locus_tag="VFX11_16300"
     CDS             complement(1603..>2792)
                     /gene="fabB"
                     /locus_tag="VFX11_16300"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX4940237.1"
                     /translation="GIVSCLGNDKATVTAALKNGTSGIRFKESYKEMGLRSHVAGQVQ
                     INVEDFIDRKPLRFMGDAAAYAYIAMQQAIADSGLTDSEVSNIRTGIVAGSGGGSSDN
                     QVQAADILREKGVKRIGPYMVPRVMTSTVSACLATPFKIKGVNYSITSACATSAHCIG
                     HAAELIQLGKQDIVFAGGGEEEHWALTMMFDAMGALSTKYNDAPSTASRAFDVTRDGF
                     VIAGGGGMLVIEEYEHAKARGAKIYAELVGYGATSDGCDMVAPSGEGAMRCMQMAMST
                     VKGPIDYINAHGTSTPVGDAAEIGAVKEVFGGKTPPISSTKSLTGHSLGAAGVQEAIY
                     SLLMLENDFIAASANIENLDEKVAGADIVRERRDNAGLRTVMSNSFGFGGTNATLVMQ
                     KPE"
BASE COUNT          626 a          718 c          762 g          686 t
ORIGIN      
        1 cgaaatagcc tttgcttttc atgcgactgt catataattg ccacgtcgtc ctgtttgcaa
       61 tagggggcgg cgctgcaaca atctgtgttt gctgtagtgc ggctggtcag ggaagtcagg
      121 atagaaaaat ggaatgggct cagtcttttt gcgatgcgac agcaggaaac tgcgagcctg
      181 gtttttgatg acccgtttta ctgtttttgg tttcccgcga aacgtgaatg tcactgaagt
      241 gtcataaatc gtcttcatac tgcgcaccgt cagtgaaaac agtcttttgt gtcaaatacc
      301 tcggaccttt aggagagaga atatgaaagt aagattgctt cctttggcta tcagcgccgc
      361 aattgccatg cctggagttg ctttagcgga aggccctact gtttatggca agctgaatgt
      421 ttcatatgaa atggttgatg ttgattacgc gtacgacgac atcgatccca acagcgctga
      481 tcggtgggac ttgctgagca atgcgtcgcg ttttggtgtg aagggtgatg cagctattaa
      541 cgacagcctg aaggcaattt accagatcga atgggaagtg ggcgcagacg aaggcaacac
      601 tttcacttcc cgtgatcgct ttgtaggtct ggctggtggg tttggtacac tgcaggcggg
      661 caagtttgat agcccactga aaaagtccca aggcaagatc gatcagttca atgacttggc
      721 aggcgacatt aaatacgttc tgctgggcga agagcgcttg gacaacgtaa ttcagtattc
      781 cacaccagta atgggtggtt tccaaggcaa tatcgcattt gcgccaggtg aagagtacga
      841 tcaaggtcag gatgatccca aagatggtcc agctgactcg ttctctctta gcggtacatt
      901 caccgtcgac gcattgtatc tcgcgttagc ttacgattct caagtcgcta ccgaagccta
      961 tacaggaata gatagtgact tcgaattgac tgataccaac gatttgattg gcgagattgc
     1021 agacgagctg gatgttgatt ctgaaactgc ctattttgat actgtaaggt tggttgctca
     1081 atacacaatg ggtgatttcc agctaggtgc gttgtatcag atggctgaaa cgtcagatcc
     1141 tcttttcacc gtgcttgatc tggaacaaga tggattcgtt gttagtggtg cgttcacatt
     1201 gggtcaaaac gtatttaagc tacagtatgg tgcgtctaca accgaggcca atgtagacga
     1261 agttgatttc tctgcagaaa acgacgctac atcgcttggt ctgggaatgg atcacaagct
     1321 cagcaagcaa acaaccctgt ttgcttatta caacgcgctg agctatgagt tcgatgatga
     1381 agacgccgaa gaacaaacca aagacaacct gggcgtaggt atcgtccaca acttctaatc
     1441 cctcccagga tcaagaagtt cccgaaaacg ccagcctcgt gctggcgttt tcatttctgc
     1501 aattgcaatc gtccgcaaga tccttcattt tctcccgccc acaaaaaagc agcccgaagg
     1561 ctgcttcaat gttcacttca gtaacggttt gccgccgcca gatcactccg gcttctgcat
     1621 caccagcgtc gcgttggtgc caccaaaccc gaaactgttg ctcatcactg tccgcagtcc
     1681 ggcattgtcc cgccgttccc gcacgatatc ggcacctgcc actttctcat ccagattttc
     1741 aatgttggcg gacgcggcga tgaaatcgtt ttccagcatc agcaggctgt atattgcttc
     1801 ctgcacgcca gctgcaccca gcgagtgacc cgtgagggat ttggtggagc tgatgggggg
     1861 tgtcttgccg ccgaatactt cctttaccgc accgatttct gccgcatcac ctaccggtgt
     1921 gctggtaccg tgggcattga tgtaatcgat ggggcctttc accgtggaca tggccatctg
     1981 catgcagcgc atcgcgcctt cgccgctggg agccaccatg tcgcagccgt cggaagtggc
     2041 gccgtagccc accagttcgg catagatttt cgcgccacgg gccttggcat gttcatattc
     2101 ctcgatgacc agcatgccac cgccgccagc aatgacgaag ccgtcacggg tgacatcaaa
     2161 ggcacgggat gcagtggaag gggcatcgtt gtacttcgtg gacagtgcgc ccatggcatc
     2221 gaacatcatg gtcagggccc aatgttcctc ctcgccaccg ccggcaaaaa cgatgtcctg
     2281 cttgccgagc tggatcagtt ccgccgcgtg gccaatgcag tgagcgctgg tggcgcaggc
     2341 agaggtgatc gagtagttca cacccttgat cttgaacggt gtggccagac aggcggacac
     2401 ggtgctggtc atgacgcgtg gcaccatata gggcccaatg cgcttgacgc ctttttcccg
     2461 caggatgtcg gcagcctgca cctggttgtc ggagctgccg ccaccggagc cggcgacgat
     2521 gccggtacgg atattggaaa cttcgctgtc ggtcaggccg gaatcggcaa tcgcctgctg
     2581 catggcaatg taggcgtagg cagcggcatc acccatgaaa cgcaggggtt tacggtcgat
     2641 aaagtcttcg acgttgatct ggacctggcc cgccacatgg ctgcgcaagc ccatttcttt
     2701 gtacgactcc ttgaagcgga tgcccgaggt gccgttcttg agcgcagctg tgaccgttgc
     2761 cttgtcgttg ccgaggcagg aaacgatgcc ca
//
LOCUS       DASUEG010000864         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_354023, whole genome shotgun sequence.
ACCESSION   DASUEG010000864 DASUEG010000000
VERSION     DASUEG010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354023"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..845)
                     /locus_tag="VFX11_16305"
     CDS             complement(129..845)
                     /locus_tag="VFX11_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4940238.1"
                     /translation="MTDPVEQQLANDLLAASIHEIKNRFGLLFAQLEQLLQSLPLNDN
                     HSHSADLIQDEAQFIGSELVRILASYKSLDSSTSANLNQQFLLDFLEEKVARHASTAR
                     ARGLRIDFECDEDLAAFFDAGIIGIVLDTAIYNAAKEGAKTILLTAEHEPGSGELTIQ
                     VHDDGPGFPPAMLEAAASGPGAIKAESGSTGLGLHFARRLLALHREAGEAGDSSRCGT
                     LTLGHSDRLGGACVTLHLPQ"
     gene            complement(842..2482)
                     /locus_tag="VFX11_16310"
     CDS             complement(842..2482)
                     /locus_tag="VFX11_16310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940239.1"
                     /translation="MNMDLIPYHKKKVLIIEDLAEMRSTLKSMLANMGVQSMETVNNG
                     EEALAKLALNQYDIIFSDYELGRGKDGQQILEEVRHSGLIPASTVYIMVTAAQTVEMV
                     MGALEFEPDGYITKPVTLEILRTRLNRIIRTKDVYQEINRAIDRKDINGALEACNRLA
                     VENPKFALPAYRIKGKLLLNENRIEEARDIYDTVLGIKRVAWAMLGMAKVHYLQDQLD
                     DAQKLLEGLANTNVKYVEAFDWLAKVLEAQGKLKSAQAVLEKAVEQSPKAVLRQQELA
                     RVAELNGDYETLHKACRKANALAKNSVFRNADLLIRYAKSMQPKIKHGSMRDQKLAIS
                     EAMSLLETAKTEFQLNIAQTLKCALVEAQTLFNSGKEIEGKLAYKAAVTMLKNEELLS
                     LDDRLDVFAAKLQFEDSSDAAAYGAKLLAQIGNNRRLQANYYRVMDFHLSKQPEERLA
                     LLKKRGQELMERNDFEEAFDVLQRAARAANTDADSQVSALTAGVNLYRQGTRSTELLE
                     DVNSLFKALENLNTGDPRYPALEKLRLQWSQQTSGTPS"
     gene            complement(2579..>3020)
                     /locus_tag="VFX11_16315"
     CDS             complement(2579..>3020)
                     /locus_tag="VFX11_16315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940240.1"
                     /translation="GANARFLAQRGLHCTGWDISQVALDQLARWRGDDGAQVYTQLRD
                     VEAEPPAPASFDVIVVSDFLHRPLCNALSAALRPGGLLFYQTWCADKCATAGPSNPDF
                     LLQTNELPSLFTGLEIRFYQHFSRAGDLTLGDRDRACLVAQQPG"
BASE COUNT          515 a          882 c          945 g          678 t
ORIGIN      
        1 cacatggatg tgcttgtagc gtgtcctgaa acgccttcac ccgctcatgc tgcctagtgt
       61 ggcagtaagc ccaaaacccg gcattatagc ctgaagtctt taccgattgg ggattgaaac
      121 gtgccgcgct actgcggcag atgcagggtg acgcaggcac caccgaggcg gtcgctgtgg
      181 cccagggtca gggtgccgca acgggaggaa tcgccggcct cccccgcttc ccggtgcagt
      241 gccagcaggc gacgggcgaa atgcaggccc aggccggtgg aaccggattc ggccttgatg
      301 gcgccgggtc cggatgccgc cgcttccagc atggccggcg gaaaacccgg accgtcatcg
      361 tgcacctgga tcgtcagttc gccactgccc ggctcgtgtt ccgccgtgag cagaatggtt
      421 ttggcgcctt cctttgccgc attgtagatg gcggtgtcca gcacgatacc gatgatgccg
      481 gcatcgaaaa aggcggccag atcctcgtca cactcaaagt cgatgcgcag gccccgtgcg
      541 cgggcggtgc tggcatggcg ggcgactttc tcttccagaa aatccagcag aaactgctgg
      601 ttcaggttgg cactggtact gctgtcgagc gacttgtaac tggccaggat acgcaccagt
      661 tcgctgccaa tgaactgcgc ctcgtcctgg atcagatcag cgctatggct gtggttatca
      721 ttcagtggca gggattgcag taattgctca agctgggcaa acagcagccc gaaacggttc
      781 ttgatttcat ggatcgacgc cgccagcaga tcgttcgcca gctgctgctc caccggatcg
      841 gtcatgacgg ggtaccgctg gtctgctgcg accattgcag gcgcagcttt tccagtgcag
      901 ggtagcgcgg atcgccggtg ttgagattct ccagcgcctt gaacaggctg ttcacatcct
      961 ccagcagctc ggtactgcgg gtaccctgcc ggtacaggtt gactccggcg gtgagcgcgg
     1021 acacctggct gtcagcgtcg gtattggcgg cgcgcgctgc ccgctgcagc acgtcaaagg
     1081 cttcctcgaa atcgttgcgt tccatcagct cctggccccg ctttttcagc agtgccagac
     1141 gttcctcggg ctgcttggac aggtggaaat ccattacccg gtaatagttg gcctgcagcc
     1201 gccggttatt gccgatctgt gccagcagtt ttgcaccgta ggctgcggcg tcgctgctgt
     1261 cttcaaactg cagcttggcg gcgaatacgt ccaggcgatc gtccagcgac agcaactcct
     1321 catttttcag catcgttacc gcagctttat aggccagctt gccttcgatt tccttgccgc
     1381 tgttgaagag ggtctgggcc tccaccagcg cgcatttcag cgtttgcgct atgttgagct
     1441 gaaattccgt tttggcggtt tccagcaatg acatggcttc actgattgcc agtttctggt
     1501 cgcgcatgct gccgtgcttg attttcggct gcatggactt ggcataacgg atcagcaggt
     1561 cggcgttccg gaacacggaa ttcttggcca gcgcgttggc cttgcggcag gctttgtgca
     1621 gcgtttcgta gtcgccgttc aattcggcga cgcgggccag ttcctgttgc cgcagcacag
     1681 ccttgggcga ttgctccacg gctttttcca gtacggcctg cgccgacttc agtttgccct
     1741 gagcttcaag caccttggcc agccaatcaa aggcttccac gtatttgacg ttggtgttgg
     1801 ccaacccttc cagcagcttt tgtgcatcgt ccagctggtc ctgcaggtag tgcaccttgg
     1861 ccatgcccag cattgcccag gccacccgct tgatacccag cacggtgtcg taaatgtcgc
     1921 gggcttcctc gatgcggttt tcgttcagca gcagcttgcc tttgatgcgg taggccggca
     1981 gtgcgaattt gggattttcc accgccagcc ggttgcaggc ttccagcgcg ccgttgatgt
     2041 ccttgcggtc gatggcgcgg ttgatttcct ggtacacgtc cttggtacgg atgattctgt
     2101 tcaggcgggt acgcaggatt tcgagggtca ccggtttggt gatatagccg tcgggttcga
     2161 actccagcgc gcccatgacc atctccaccg tctgtgcggc ggtgaccatg atgtacacgg
     2221 tggatgcggg aatcaggcct gaatgacgta cctcttccag gatctgctgg ccatccttgc
     2281 cacggcccag ttcgtagtcg gagaagatga tgtcgtactg gttcagtgcc agcttggcca
     2341 gtgcctcctc gccattgttg acggtttcca tggactgtac gcccatgttg gcaagcatgg
     2401 atttcagggt ggagcgcatc tccgccagat cttcgatgat cagcactttt ttcttgtggt
     2461 acggaatcag gtccatattc atggcagtgg cacgacgtcc tgcgcagatg gctacttacc
     2521 tgataagtgt aggagaaatg gccggctgcg ccagtgtggt gccggcgctg ccccggcgtc
     2581 agcctggttg ctgggccacc aggcaggcgc ggtcgcggtc acccaaggtc agatcgccgg
     2641 cccgcgagaa atgctggtag aagcggattt ccaggccggt gaagaggctc ggcaattcgt
     2701 tggtctgcag caggaaatcc ggattggacg ggccggcggt agcgcacttg tcggcgcacc
     2761 aggtctgata gaacagcaga ccgccgggac gcagggcggc gctgagggcg ttgcagagcg
     2821 ggcggtgcag gaaatcgctg acgacgatga cgtcgaagct ggcgggcgcc ggtggttcgg
     2881 cttccacgtc gcgcagctgg gtgtagactt gcgcgccatc gtcaccgcgc cagcgcgcca
     2941 gttggtcgag ggccacctgg ctgatgtccc agccggtgca gtgcaggccc cgttgcgcga
     3001 gaaaacgggc attggcaccc
//
LOCUS       DASUEG010000865         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_652391, whole genome shotgun sequence.
ACCESSION   DASUEG010000865 DASUEG010000000
VERSION     DASUEG010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652391"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFX11_16320"
     CDS             complement(<1..536)
                     /locus_tag="VFX11_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HEX4940241.1"
                     /translation="MSQAATQYSLAELMIVAGAEALRHDGEVLATGIGVLPRLAASLA
                     MKTFNPDLMMTDSEAFILSEPNPVSGRSSHEGQKQESWMGFSRIFDNVWSGKRHAIVG
                     PSQIDRYGQTNISALGGSYDKPKSQMLGVRGFPGNSISHANSFFVPAHSKRVFVEGEC
                     DVVCSIGYNPQRLPKGYAL"
     gene            complement(569..1444)
                     /locus_tag="VFX11_16325"
     CDS             complement(569..1444)
                     /locus_tag="VFX11_16325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX4940242.1"
                     /translation="MNKVRTAQEAVAQIESGMTVGIGGWGPRRKPMALVRELLRSDVK
                     DLTIVAYGGADVGLLCAAGKVKKVVFAFVSLDFIPLEPYFREARQNGTVETMEIDEGM
                     MLLGLRAAAWGVPFIPTRIGLGTDVIKFNKDLKVIGSPYDDKEWVAMPALNLDVALIH
                     VDRADERGVCQIKGPDLYMDDWFARAAKNTIVSCDELVPTEYFHAGDEARYVHWERSQ
                     TQMVVPAPGGAHPSSCNPLYGYDTAHFNEYNASVKDGGFSRYFEKYIAGKSESEYQMA
                     VGGLEKIRALALPVY"
     gene            1680..3281
                     /locus_tag="VFX11_16330"
     CDS             1680..3281
                     /locus_tag="VFX11_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008004410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HEX4940243.1"
                     /translation="MKNPSHERAQDTGPVATIPQLVQRAARLFGPQTFLEEDGTRISF
                     GDFAQQARQVAAALLARGIQTGDRVAIWAPNISEWVIAAIGAQCAGAVLVTLNTRYKG
                     SEAAYILRQSRARLLFSIDRFLNCDYPAMLQGEALPDLQGLIVFRAAPDTTSSHTRWE
                     DFLEQATATAPALVQQRMDAIAGSDLSDILFTSGTTGNPKGVMTSHEQNLRAFTSFAD
                     ILGLCSGDRYLVINPFFHSFGYKAGILTCLIKGVTLLPHAVFDASDILTRIARDRISV
                     LPGPPTLFQSLLAHPALDQHDLSTLKRATTGAAVIPVEMIHQMKKRLGFATVITAYGL
                     TESCGLVTMCRAGDSAEIIATTSGRAIPDVELRCVDASNQEVPRGEPGEIVFRGFNVM
                     QGYFENAAASAEVIDRDGWLHTGDVGVMDAEGNLKITDRLKDMFITGGFNCYPAEIEK
                     IIAGHSAVTMNAVIGIPDERMGEVAMAFVVKKPGAALDEAGLIAWCRDQMANFKVPRK
                     VRFVDALPLNASGKVVKADLRKLAQ"
     gene            3335..>3750
                     /locus_tag="VFX11_16335"
     CDS             3335..>3750
                     /locus_tag="VFX11_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biphenyl 2,3-dioxygenase"
                     /protein_id="HEX4940244.1"
                     /translation="MKIHSLGYIGVNCTDPARWSDYGTKVLGMEDVSAQLRAPGDSSV
                     FLKMDDRPFRIIVSQSGQDNFGFSGWEIAGPVAFAEAVQTLEKHGVNFERAGADVLAA
                     RRVQELVSFADPDGNRHEIYWGPVSDFRQFASPVGI"
BASE COUNT          754 a         1212 c         1069 g          715 t
ORIGIN      
        1 agcgcgtaac ctttcggcag ccgctgcggg ttgtagccaa tggaacagac cacatcgcac
       61 tcgccttcca cgaacacacg cttgctgtgg gctggcacga aaaacgagtt ggcgtggctg
      121 atggaattgc ccggaaaacc gcgcacgccc agcatctgcg atttgggctt gtcgtagctg
      181 ccaccgaggg cggaaatatt ggtctggccg taacggtcga tctgcgacgg gcccacaatg
      241 gcgtgccgct tgccgctcca cacgttgtcg aaaatgcgcg aaaaacccat ccagctttcc
      301 tgcttctgcc cttcgtgcga gctgcggccg ctgacgggat tcggttccga cagaatgaag
      361 gcttcggaat ccgtcatcat cagatccgga ttgaacgttt tcatcgccag gctggcggcc
      421 aggcgcggca atacgccgat gccggtggcc agcacttcgc cgtcatgacg cagcgcttca
      481 gcgcccgcca cgatcatcag ttctgccaga ctgtactggg ttgctgcttg actcattgat
      541 atttccccat tgctcagact ggcacggatc agtaaaccgg aagcgccagg gcgcggattt
      601 tttccagacc gcctaccgcc atctggtatt cggattcgct cttgccggcg atgtattttt
      661 cgaaatagcg gctgaagcca ccatccttca ccgacgcgtt gtattcgttg aaatgcgcgg
      721 tgtcgtaacc gtacagcgga ttgcaggagg acggatgggc gccgccggga gccggcacca
      781 ccatctgggt ctgcgaccgt tcccaatgca cgtagcgggc ttcgtcgccg gcatgaaaat
      841 actcagtggg caccagttcg tcgcagctca caatggtgtt tttcgcggca cgggcaaacc
      901 agtcgtccat atacaggtcg gggcccttga tctggcacac accacgctcg tcggcgcggt
      961 ccacgtgaat cagcgccaca tccagattca gcgcgggcat tgccacccat tccttatcgt
     1021 cgtaaggact gccaatcact ttcagatctt tattgaattt gatgacatcc gtgcccagac
     1081 caatccgggt gggaatgaag ggcacacccc aggccgcagc gcgcaggccc agcagcatca
     1141 tgccctcgtc gatttccatc gtctcgacag tgccgttctg gcgcgcctcg cggaaataag
     1201 gctccagcgg aatgaaatcc agcgacacga atgcgaacac cactttcttc accttgccgg
     1261 ccgcgcacag caggcccaca tcggcgccgc cataagcaac gatggtgagg tctttcacat
     1321 cggaacgcag cagctcgcgc accagcgcca tcggtttgcg ccgggggccc cagccgccga
     1381 tgccgacggt catgccgctt tcgatctgcg ccaccgcttc ttgtgccgtt cgcactttat
     1441 tcatcggatt ctctctgtag ttcgtatcct gggttccgct ctgatgtccc agtgcgtgat
     1501 tccggccaag gccatcgcag ccgaaactgt ccaaaaaaca acggtaattt cccaatcttc
     1561 ccggtcatta tggggggttc aagcaggggg aataatcgtc caagtggact aggacgcccg
     1621 cgccaccgtt ccgctacctt ttgcctggtc gacaacaaga agaaacaggt gcaggctgca
     1681 tgaaaaaccc gtcccacgaa cgcgcccagg acactggccc cgtcgccacc atcccgcagc
     1741 tggtgcagcg ggccgcgcgc ctgttcggcc cccagacgtt tctggaagag gacggcaccc
     1801 gaatcagctt tggcgatttc gcgcagcagg cccgccaggt ggccgccgcc ctgctggccc
     1861 gcggcatcca gaccggtgat cgcgtggcga tctgggctcc caatatcagc gagtgggtga
     1921 ttgccgccat cggcgcccag tgcgccggcg ccgtgctggt cactctgaat acccgctaca
     1981 agggcagcga ggcggcctat atcctgcgcc agagccgcgc ccgcctgctg ttcagcatcg
     2041 accgctttct caactgcgat tatccggcca tgctgcaggg cgaagcgctg ccggatctgc
     2101 agggcctcat cgtttttcgc gccgcgcccg acaccacaag cagccacacc cgctgggaag
     2161 atttcctgga acaggccacc gccaccgcgc ccgccctggt gcagcagcgc atggacgcca
     2221 tcgccggcag cgacctgtcc gacatcctgt ttacgtccgg taccaccggc aaccccaaag
     2281 gcgtgatgac gagtcacgag cagaatcttc gcgcctttac ctcattcgcc gacatcctgg
     2341 gcctgtgcag cggcgatcgc tatctggtga tcaatccgtt ttttcacagc ttcggctaca
     2401 aggccgggat tctgacgtgc ctgatcaagg gcgtgacact gctgccccac gccgtgttcg
     2461 acgccagcga catcctgacc cgcatcgccc gcgaccgcat cagcgtgctg ccggggccac
     2521 ccaccctgtt ccagtcactg ctggcacatc cggcgctgga ccagcacgac ctttccacac
     2581 tgaaacgcgc caccaccggc gccgccgtga ttccggtgga aatgattcac cagatgaaaa
     2641 aacggctggg gtttgccacc gtgatcaccg catacggcct caccgaaagc tgcggcctgg
     2701 tcaccatgtg ccgcgccggc gacagcgccg agatcatcgc caccacgtca ggccgcgcga
     2761 ttccggacgt ggaactgcgc tgcgtggatg ccagtaacca ggaagtgccc cggggcgaac
     2821 cgggcgaaat cgtgtttcgc ggtttcaatg tgatgcaggg ctattttgaa aatgccgccg
     2881 cctctgccga ggtaattgat cgcgacggct ggctgcatac cggcgacgtc ggcgtgatgg
     2941 acgctgaagg caatctgaaa atcaccgacc gcctgaaaga catgttcatc accggcggct
     3001 tcaactgcta tccggcagaa atcgaaaaaa tcattgcagg ccacagcgcc gtcaccatga
     3061 acgccgtgat cggcataccg gacgagcgca tgggcgaagt ggcgatggcc ttcgtggtga
     3121 aaaagcctgg cgccgcgctg gatgaagccg gcctgattgc ctggtgtcgc gaccagatgg
     3181 ccaatttcaa ggtaccgcgc aaggtacgct tcgtggatgc gctgccgctg aatgcatccg
     3241 gcaaagtcgt caaggcagac ctgcgcaaac tggcgcaata gaaaatggtt tattccatag
     3301 cgggatacac actccaacaa aaaggaatgt tcagatgaaa attcacagcc tcggctacat
     3361 tggcgtcaac tgcaccgacc ccgcccgctg gtccgactac ggcaccaaag tgctgggcat
     3421 ggaggatgtg tcggcgcaac tgcgcgcacc cggtgacagc agcgtattcc tgaaaatgga
     3481 cgaccgcccc ttccgcatca tcgtcagcca atccggccag gacaacttcg gtttcagcgg
     3541 ctgggaaatt gccggccctg ttgcctttgc cgaagcggta cagactctgg aaaaacacgg
     3601 cgtgaatttc gaacgcgccg gtgccgacgt actggcggcg cgccgcgtgc aggaactggt
     3661 gagctttgcc gatcccgatg gcaaccgcca cgaaatctac tggggcccgg tgtccgactt
     3721 ccgccagttc gcctcgcccg ttggcatcaa
//
LOCUS       DASUEG010000866         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1832656, whole genome shotgun sequence.
ACCESSION   DASUEG010000866 DASUEG010000000
VERSION     DASUEG010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832656"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3346)
                     /gene="gltB"
                     /locus_tag="VFX11_16340"
     CDS             complement(<1..>3346)
                     /gene="gltB"
                     /locus_tag="VFX11_16340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX4940245.1"
                     /translation="KLKEDRDFYVASLSATIVSYKGLMMPKDLPAFYLELADARMETA
                     ICVFHQRFSTNTLPRWPLAQPFRFLAHNGEINTIMGNRNWSVARTPKFASELLPDFDD
                     IKPLVNRTGSDSSSLDNMLELLVVGGMDIFRAVRMLVPPAWQNVETMDADLRGFYEYN
                     SMHMEPWDGPAGLVMTDGRHAICMLDRNGLRPSRWVITQNGYITLASEIGVHAYRPED
                     VVAKGRVGPGQILVVDTETGELLHTADIDNRLKARKPYKQWLKDNAIRIQDTLTEETR
                     QLEQLDPEAIKTYQKMFQVSFEERDQVLRPMAETAQEAVGSMGDDTPMAVLSTKIRPL
                     YDFFRQQFAQVTNPPIDPLREAIVMSLETCLGAERNVFEETAEHADRAILTSPVLSHS
                     KFQAVTNIKRKGYEVARIDLNYDEAISLRQAVINVADAAEQAVRSGKVILVLTDRAIQ
                     QGKLPVHALLAVGAVHNRLTQKGLRCDSNIVVETATARDPHHFACLIGFGATAVYPYL
                     AYDVLCDLVRTGEILMDPIDAQKNFRKSIDKGLLKILSKMGISTVASYRGGQLFEAVG
                     IASDVREICFSDVPSRIEGAGFVHFEADIKALAADAWKARKPIEQGGLLKFVHGGEYH
                     AFNPDVVAALHKAVQSGEYEAYKEYAALTQDRPVAAIRDLLKLKSGLKPIPLEDVEPL
                     EKILRRFDSAGMSLGALSPEAHEAIATAMNTLGGRSNSGEGGEDPARFGTIKNSKIKQ
                     VASGRFGVTPAYLTSAEVLQIKIAQGAKPGEGGQLPGGKVNELIARLRYSVPGVTLIS
                     PPPHHDIYSIEDLSQLIFDLKQVNPDALVSVKLVSEPGVGTIAAGVAKAYADLITISG
                     YDGGTAASPLTSIRYAGSPWELGLSEAHQALRANDLRAKVRLQTDGGLKNGLDVIKAG
                     IMGAESFGFGTIPMLALGCKYLRICHLNNCATGVATQQDKLRKDHFIGEPEMVMNFFK
                     FVAQETREWLARIGVRSMEELVGRTDLLEIIEGITPKQKALNLRPLLVNDNIPADKPQ
                     TCGMPRNEPFDKGILAEKMVSAAISAIEKKAGGEFEFRITNCDRSIGARISGEIAKRH
                     GNQGMADKPITFRFKGTAGQSFGVWNA"
BASE COUNT          601 a         1056 c         1029 g          660 t
ORIGIN      
        1 cggcgttcca cacgccgaag ctctggcccg ctgtgccttt gaagcggaaa gtgatgggct
       61 tgtccgccat gccctggttg ccgtgacgct tggcgatttc accggaaatc cgcgcaccga
      121 tggagcggtc gcagttggtg atgcggaatt cgaattcgcc accggctttc ttctcgatgg
      181 cggagatcgc cgcgctgacc attttctcgg ccagaatgcc cttgtcgaac ggttcgttgc
      241 gcggcatgcc gcaggtctgc ggcttgtcgg caggaatgtt gtcgttcacc agcagcggac
      301 gcaggttcag cgccttctgc ttgggcgtga tgccctcgat gatttccagc agatcggtgc
      361 gacccaccag ctcttccatc gagcgcacgc cgatgcgggc cagccactcg cgggtttcct
      421 gcgccacgaa cttgaagaag ttcatcacca tttccggctc gccgatgaaa tggtccttgc
      481 gcagtttgtc ctgctgggtg gcaacgccgg tggcgcagtt gttcaggtga cagatccgca
      541 agtatttgca gcccagcgcc agcatcggaa tggtgccgaa gccgaagctt tccgcgccca
      601 tgatgccagc cttgatcacg tcgaggccgt tcttcaggcc accatcagtc tgcaggcgta
      661 ccttggcgcg caggtcgttg gcgcgcaatg cctggtgcgc ttcgctcaga cccagttccc
      721 acggcgagcc ggcatagcgg atcgaggtga gcgggctggc ggcggtaccg ccgtcataac
      781 cggaaatggt gatcaggtcg gcgtacgcct tggccacacc ggcggcgatc gtgcccacgc
      841 cgggttccga caccagcttc accgacacca gcgcatccgg attgacctgc ttcaggtcga
      901 aaatcagctg cgacaaatct tcgatggaat agatgtcgtg atgcggcggc ggcgagatca
      961 gggtcacgcc gggcacggaa taacgcaggc gcgcgatcag ctcgttcacc ttgccaccgg
     1021 gcaactggcc tccctcacca ggcttggcgc cctgggcaat cttgatctgc agcacttccg
     1081 cactggtcag atacgccggg gtcacgccga aacggcccga cgccacctgc ttgatcttgg
     1141 aattcttgat ggtgccgaaa cgggccggat cttcaccgcc ctcaccggaa ttggaacggc
     1201 cacccagcgt gttcatcgcg gtggcgatgg cctcgtgggc ttccgggctc agcgcaccca
     1261 gcgacatgcc ggcggaatcg aagcggcgca ggattttttc cagcggctcc acgtcctcca
     1321 gcggaatcgg cttgaggccg gacttcagtt tcagcaggtc gcgaatcgcc gccaccggac
     1381 gatcctgggt cagcgccgcg tattccttgt aggcctcgta ttcgccggac tgcacagcct
     1441 tgtgcagcgc cgccaccaca tccgggttga aggcatggta ttcgccaccg tggacaaatt
     1501 tcagcaaacc gccctgctcg atcggcttgc gcgctttcca cgcatccgcc gccagcgcct
     1561 tgatgtctgc ttcaaaatgg acaaagccgg cgccttcgat gcggctcggc acgtcgctga
     1621 aacagatctc gcgcacgtcg gacgcgatgc ccaccgcttc gaacagctga ccgccacgat
     1681 aggacgccac cgtggaaata cccatcttcg acaggatctt caacagaccc ttgtcgatgg
     1741 acttgcggaa attcttctgc gcatcgatcg ggtccatcag gatctcaccg gtacgcacca
     1801 gatcgcacag cacgtcgtac gccagatacg gatacacggc ggtggcgcca aaaccgatca
     1861 gacaggcgaa atgatgcgga tcgcgggcag tcgcggtttc caccacgata ttggaatcgc
     1921 agcgcagccc cttctgggtc agacggttgt gcaccgcgcc caccgccagc aatgcgtgca
     1981 ccggcagttt gccctgctgg atcgcgcggt cggtcagcac caggatgacc ttgccggaac
     2041 gcacggcctg ctcggcagcg tccgccacat tgatcaccgc ctgacgcagg ctgatggcct
     2101 cgtcgtaatt caggtcgatg cgggcgactt catagccctt gcgcttgatg ttggtaaccg
     2161 cctggaactt gctgtgggac agcaccggcg acgtcagaat cgcgcggtcg gcgtgctcgg
     2221 cagtttcctc gaacacattg cgctcggcac ccagacaggt ttccagcgac atcacgatcg
     2281 cttcccgcag cggatcgatc ggcggattgg tcacctgcgc aaactgctgg cggaagaagt
     2341 cgtacagcgg acgtatcttg gtggacagca ccgccatcgg ggtgtcgtcg cccatggagc
     2401 ccaccgcttc ctgcgcggtt tccgccatcg gacgcagcac ctggtcccgc tcctcgaacg
     2461 acacctggaa catcttctgg taagtcttga tcgcctccgg gtccagttgc tccagctgcc
     2521 gggtttcctc ggtcagcgtg tcctggatgc ggatggcgtt atccttcagc cactgtttgt
     2581 agggcttgcg ggccttcagg cgattgtcga tgtcggcagt gtgcagcagc tcgccggtct
     2641 cggtgtccac caccagaatc tggccgggtc cgacgcggcc tttggccacc acatcttccg
     2701 gccgataggc atgcacgccg atctcggacg ccagcgtgat gtagccgttc tgggtgatca
     2761 cccaacggga cggacgcaga ccgttgcggt ccagcatgca gatggcgtga cggccatcgg
     2821 tcatcaccag accggcgggg ccgtcccagg gctccatgtg catggagttg tattcgtaga
     2881 agccgcgcag atcggcatcc atggtctcga cgttctgcca ggccggcggc accagcatgc
     2941 gcacggcacg gaagatgtcc atgccaccga ccaccagcag ttccagcatg ttgtcgaggc
     3001 tggaggagtc ggaaccggtg cggttcacca gcggcttgat gtcatcgaaa tcaggcagca
     3061 gttcggatgc gaatttgggc gtccgcgcca cagaccagtt gcggttgccc atgatggtgt
     3121 tgatttcacc gttgtgcgcc aggaagcgga acggctgcgc cagcggccag cgcggcaggg
     3181 tattggtgga gaaacgctgg tggaagacgc agatcgcggt ctccatgcgc gcgtcggcca
     3241 gctcgaggta gaaggctggc agatccttcg gcatcatcaa ccctttgtag gagacgatgg
     3301 tggcggacag gctggccacg tagaaatcgc ggtcttcctt cagttt
//
LOCUS       DASUEG010000867         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1429977, whole genome shotgun sequence.
ACCESSION   DASUEG010000867 DASUEG010000000
VERSION     DASUEG010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429977"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VFX11_16345"
     CDS             <1..1217
                     /locus_tag="VFX11_16345"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4940246.1"
                     /translation="RSDNTASAAIACAALLGEGDPLRFDGDNGQANIHLRLALWQSSS
                     PHNVGHGQLQRGTWQRIQQLVQQTAKRVQTQWNPASVDHADIAGALAQAFPDRVAQLR
                     ADSQSRYRMANGKGVQLGRHDALAGSQYLVILETDGAGNEPRVRLACALTLAQLKSAL
                     ATHLQQQDVISWNEQRGAVEAERQLQFGALVLERSRLRQPWPDAAQQCLLQALQKNAL
                     DDLPWNDNTTQLLARLQWLHRQQPDQWPDCSRTALSESIQDWLLPFLNNIYSLTEVQK
                     INLHDALKNRLEWNQWQALDTQAPTAIALPAGTTRELDYLAENGPVLRARLQEFYGLN
                     VHPTLPNGQRILLELLSPAQRPIQVTRDLPGFWHGSYREVAKDMRGRYPKHFWPDDPA
                     SAVATTKTKRNM"
     gene            complement(1223..2296)
                     /locus_tag="VFX11_16350"
     CDS             complement(1223..2296)
                     /locus_tag="VFX11_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940247.1"
                     /translation="MNPGPLHLLRCLPGLLLISLLTACGGGGGGGDDDEIGSFNSAIS
                     LDRRISAARATASNSSACTAIEPFYWEIGDRSDRITGSSEGDGSVLASTDMKIASASK
                     WIFGAYVLEKRGNVLTADDIKALTMRTGYSEFRYGLCIEGTQVQQDTLTVQQCFEKSA
                     SGTANNDYVAGDDGKFNYNGGHFQYWAVQNGLEDDINETLAIELGDQFGNAFALDFDS
                     PQLAGGLYMNALNYAFFLRSILSDDLYMHDRLGTDAVCTSPAVCPDTATYTPIPADEA
                     WHYSLGHWVEDDPVVGDGAFSSPGYFGFYPWIDSGRKWYGIVARGVEPGNLLEFLFGD
                     TAMESVDCGRRIRKAWLSGQQQN"
     gene            2450..>3760
                     /locus_tag="VFX11_16355"
     CDS             2450..>3760
                     /locus_tag="VFX11_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX4940248.1"
                     /translation="MTTPQTLPQTATLDGNEAVALIAHKCNEVIAIYPITPASAMGEF
                     ADAWSAQGRRNLWGSVPQIVEMQSEGGAAGAVHGALQGGALCTTFTASQGLLLMLPNL
                     YKIAGELTPTVFHIAARSLASHALSIFCDHSDVMSARGTGFALVASNSVQEAHDFAAI
                     TQAVTLRSRIPVLHFFDGFRTSHEVAKIDLVEDDDLRALLDGPFNQNAIAEHRRRALS
                     PDHPVLRGTAQNPDVFFQAREACNPFYDRFPALLQECMDAFATRTGRAYHLYDYVGAP
                     DAEQVIVLMGSGAETVHETVLQLNAGDAKTGVLKVHLFRPFSAKHFLAALPSSVKSIA
                     VLDRTKEPGAAGEPLYQDVCTVFMEAFNSGVRTSLPKIIGGRFGLSSKEFTPAMVRAV
                     FAELRAPAPKNHFTVGIEDDVTHRSLPYAMYETANPTANDDSSND"
BASE COUNT          746 a         1210 c         1140 g          664 t
ORIGIN      
        1 agcgcagtga caacaccgcc agcgccgcga ttgcctgcgc cgccctgctg ggggaaggcg
       61 atccgctgcg cttcgacggc gacaacgggc aggcaaatat tcatctgcgg ctggcgctgt
      121 ggcagtcatc atcaccgcac aacgttggcc acggtcagct gcagcgcggc acctggcagc
      181 gcatccagca actggtgcag cagacggcga aacgcgtgca gacccaatgg aatcccgcca
      241 gcgtcgatca tgccgatatt gccggcgcac tggcgcaggc ctttccggac cgtgttgccc
      301 agctgcgcgc cgacagccag tcgcgttatc gcatggccaa cggcaagggc gtgcagctgg
      361 gccgtcacga tgcactggcc ggttcgcagt atctggtgat tctggaaacc gatggcgccg
      421 gcaacgaacc gcgcgtgcgg ctggcctgcg cactgacact ggcgcaacta aaatcagcac
      481 tggccaccca tttgcagcaa caggacgtga tcagctggaa cgaacagcgc ggcgcggtgg
      541 aagcggaacg gcagctgcag ttcggcgcgc tggtgctgga gcgcagccgt ctgcggcaac
      601 cctggccgga tgcagcgcag caatgtctgc tgcaggcact gcaaaaaaat gccctcgacg
      661 atctgccgtg gaatgacaac accacgcagc tgctggcgcg cctgcaatgg ctgcaccggc
      721 agcagccgga tcaatggccc gactgttcgc gcacagcttt aagcgaatcc attcaagact
      781 ggctgctgcc gtttctgaac aatatctaca gcctgacaga agtgcagaaa atcaatctgc
      841 acgatgccct gaaaaaccgg ctggagtgga atcagtggca ggcactggac actcaggcgc
      901 ccacagccat cgcactgccg gccggcacca cgcgggaact ggattatctg gcggaaaacg
      961 gtccggtgct gcgggcgcgg cttcaggaat tctacggttt gaatgtgcat ccgacgctgc
     1021 ccaacgggca gcgcattctg ctggaattgc tgtcaccggc gcagcggccg attcaggtga
     1081 cgcgggatct gccgggcttc tggcacggca gctacaggga agtggcgaag gacatgcgcg
     1141 gccgctatcc gaaacatttc tggccggacg atccggccag cgctgtcgcc accactaaaa
     1201 ccaaacgcaa tatgtagccg gatcagttct gctgctgtcc gctcaaccag gccttgcgga
     1261 tgcggcgacc gcaatcgacc gactccatcg cggtgtcacc aaacagaaat tccagcagat
     1321 tgcccggttc cactccccgc gccacgatgc cataccattt cctgcccgag tcgatccagg
     1381 gataaaaacc gaaataaccg gggctgctga aggcgccgtc acccacgacc ggatcgtctt
     1441 ccacccagtg gccaagggaa tagtgccagg cctcgtcggc gggaatcggt gtgtaggtgg
     1501 cggtgtcggg acagacggcg gggctggtgc agacggcatc ggtgccaagc cggtcatgca
     1561 tgtacagatc gtcgctcaga atcgagcgca ggaaaaaagc atagttcagc gcattcatgt
     1621 acaggccgcc cgccagttgg ggcgaatcga aatccagcgc gaaggcattg ccgaactggt
     1681 cccccagttc gatggccagg gtttcgttga tgtcatcttc cagcccgttc tgcaccgccc
     1741 agtactggaa gtggccaccg ttgtagttga acttgccgtc gtctccggcc acatagtcgt
     1801 tgttggcggt accactggcc gatttttcaa agcactgctg taccgtcagg gtatcctgct
     1861 gcacctgggt gccctcaatg cacaggccat aacggaattc gctgtagccg gtgcgcatcg
     1921 tgagggcctt gatatcgtca gccgtcagca cattgccccg tttttccagc acataggcgc
     1981 caaaaatcca tttgctggcg gaggcgattt tcatgtcggt gctggccagc acggagccat
     2041 caccttcgct gctgccggtg atgcggtcac tgcgatcccc aatttcccaa tagaaaggtt
     2101 cgatggccgt acaggcgctg ctgttggacg ccgtggcccg ggcggcgctg atgcgcctgt
     2161 cgaggctgat ggcgctgttg aaactgccga tctcatcatc gtcgccacca ccgcccccgc
     2221 caccgcaggc ggtcaacagg ctgatcagca gcaggccggg caggcagcgc aacaggtgaa
     2281 gcgggccggg gttcatgcaa gtatctccag actggttggc aaacccgggt agcatgacgc
     2341 cagcgcgccg atgctgcaac ggtcgccacc ggtcacgaac ttgtgccaga tcaacaaccg
     2401 ggcaccgggt gcagtgtaga aacaaggtat tcaccgggag gaaacctgca tgaccacgcc
     2461 ccagaccctg ccccagactg cgacgctgga cggcaacgag gcggtggccc tcatcgccca
     2521 caaatgcaat gaggtcattg ccatctaccc catcaccccc gcgtccgcca tgggcgagtt
     2581 tgccgacgcc tggtcggccc agggccgccg caacctgtgg ggcagcgtgc cgcagatcgt
     2641 cgagatgcaa agcgaaggcg gtgccgccgg tgccgtgcac ggggccctgc agggcggcgc
     2701 cctgtgcacc acgttcaccg cgtcccaggg cctgctgctg atgctgccca acctgtacaa
     2761 gatcgccggc gagctgaccc cgacggtgtt tcacatcgcg gcgcggtcgc tggcatcgca
     2821 cgcgctgtcg attttctgcg atcacagtga cgtgatgtcg gcacggggca ccggctttgc
     2881 actggtggcc tccaactccg tgcaggaggc ccacgatttc gccgccatca cccaggcggt
     2941 caccctgcgt tcccgcattc cggtgctgca ttttttcgac ggcttccgca cgtcccacga
     3001 ggtagcgaaa atcgatctgg tggaagacga cgatctgcgc gcgctgctgg atggcccgtt
     3061 caaccagaat gccattgccg aacaccggcg gcgcgccttg tcaccggatc atccggtgct
     3121 gcgcggaacc gcccagaatc ccgatgtgtt tttccaggcg cgggaagcct gcaatccgtt
     3181 ttacgaccgc tttcctgccc tgctgcagga atgcatggac gcgttcgcca cccgcaccgg
     3241 ccgcgcctat cacctgtacg attacgtggg tgctcccgat gcggagcagg tgatcgtgct
     3301 gatgggttcg ggcgctgaaa ccgtgcacga aaccgtgctg caactgaatg cgggcgacgc
     3361 caaaaccggc gtgctgaaag tgcacctgtt ccgcccgttc agcgcgaagc atttcctggc
     3421 agcgctgccg tccagcgtaa aatccatcgc cgtgctcgac cgcaccaagg aaccgggcgc
     3481 cgccggcgag cctctgtatc aggacgtgtg caccgtcttc atggaagcgt tcaatagcgg
     3541 cgtacgcacg tcgctgccga aaatcatcgg cggccgcttc ggtctgtcat ccaaggaatt
     3601 cacccctgcc atggtgcgcg cggtttttgc cgagctgcga gcgcccgcac cgaaaaatca
     3661 tttcaccgtc ggcatcgagg atgacgtcac ccaccggtcg ctgccctatg caatgtacga
     3721 aaccgccaac ccgacggcca acgatgacag cagcaacgac
//
LOCUS       DASUEG010000868         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1027696, whole genome shotgun sequence.
ACCESSION   DASUEG010000868 DASUEG010000000
VERSION     DASUEG010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027696"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..867
                     /locus_tag="VFX11_16360"
     CDS             163..867
                     /locus_tag="VFX11_16360"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HEX4940249.1"
                     /translation="MKKPHLSRSMLMLAFLSCLACTFANAESGEWERRFSQDGLSVYT
                     KHIEGAPIKSFRAEMIAEAPFQLVMSVLEDVQHYPDWFHLCRSYEVLQGSMKEGEYIG
                     YYVVEAPWPLKDRDVYVKNRMSQDPETQTVTILTDAVPDFMPVKEEFTRVPEVYGRWT
                     VRPVDSEHTYVEFIGHGHPGGIIPLWVANLVVTDVPKKTFENLRSLFEKRLANQTGMN
                     ERAVSFQTIELTRSPH"
     gene            complement(919..2379)
                     /locus_tag="VFX11_16365"
     CDS             complement(919..2379)
                     /locus_tag="VFX11_16365"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX4940250.1"
                     /translation="MNAVNPASAFIRTLALRVLCLLVFLLMCPVHAAVVVPEPVIKSI
                     AYDAGSKQHPFVASILDLSALGYVESEFQMSGSANTYGRAGKWRSDGKWDVKTVRANV
                     PYATRLLVRRPADPAKFNGIVVVEWLNNTAIMDVDVIWAQSHEELMREGYAWVGVSAQ
                     TLGVRALRAWDRQRYGELRLTTDGVSYDVFSQAALAVRSQSESLLGGLPVAGVIGAGE
                     SQSAIRLTTYVNAFQAAAVQVFDAMLLYSRFPLSAPLKDGITALAPSKVYIRADNALK
                     ILQLETEQDVFMFRYRISRQDDTELLRTWELAGASHYDDYGVNQLLPQYQRDFPQLGS
                     VQLVCRNSLNKIPSHYVVNAALSSLTRWITTGQAPPQSPRLEYANGKVIRDANGNAVG
                     GIRLPHMDAATATNNYNNYGSIGAGGNFFVNAFACPFLGNTVPFSRAKLQALYPTHQD
                     YVSKFKAAADAALNAGFMVQADYEEAVAEAEVSAVP"
BASE COUNT          576 a          673 c          655 g          532 t
ORIGIN      
        1 actgtactca gccattgaaa ctggcactgg tcttggagct gagtggcggc aacattgatt
       61 acaaacacga ttgggtttta agatgatttc cagggccctg cttcccgcgg gcgcatttgc
      121 cactgaacct gtgtttacca aaaatcagaa caaggacaca ccatgaaaaa accacatctt
      181 tcccgcagca tgctcatgct tgcatttctg tcctgcctgg cctgtacatt cgcaaatgcg
      241 gaatccggcg agtgggaacg ccggttttca caagatggcc tgagcgtcta caccaaacat
      301 atcgagggag caccgatcaa atcgtttaga gctgagatga ttgcggaggc gccctttcag
      361 ctcgtcatgt ccgtactgga agacgttcaa cattatcctg actggtttca cctctgcaga
      421 tcctatgaag ttctccaggg atcgatgaaa gaaggcgagt atataggcta ctacgtggtc
      481 gaggcgccct ggccgcttaa agatcgggat gtttacgtca agaacagaat gagtcaggac
      541 ccggaaacgc agactgtcac catattgacg gacgccgttc cggatttcat gccggtaaaa
      601 gaggaattca cccgggttcc ggaggtgtat ggccgatgga ccgtcagacc ggtagatagc
      661 gagcatacct acgtggaatt cattgggcat ggacatccgg gtggaatcat tcctctatgg
      721 gttgccaatc tggtggttac ggatgtcccc aagaaaacgt ttgagaacct tcgttccctg
      781 ttcgaaaagc gattggcaaa ccagaccggc atgaacgaaa gagccgtatc ctttcagacc
      841 attgaactga ccagatcgcc acattgaacc cacccagcga aacaggtcgt gcagaagtaa
      901 tactggccct ggccggtatt acggcacggc tgagacctct gcttcggcaa ccgcttcttc
      961 ataatccgcc tgcaccatga atccggcgtt gagcgccgca tccgctgccg ctttgaattt
     1021 cgacacataa tcctgatggg taggatacaa cgcctgcagc ttggcacggc tgaacggcac
     1081 ggtattaccc agaaacggac atgcgaatgc attgacaaag aagttgccgc ccgcgccaat
     1141 gctgccgtag ttgttgtaat tattggttgc cgtcgctgca tccatatgcg gcaggcgaat
     1201 gccacccaca gcattgccgt tggcatcacg gatgactttt ccattcgcgt attcaagacg
     1261 aggtgactga gggggcgcct ggcccgtcgt aatccacctg gtcaaactgg agagcgcggc
     1321 attgaccacg tagtgcgatg gaatcttgtt gagtgaattc ctgcaaacca gctgaacgga
     1381 gccaagctgg ggaaaatccc gttgatactg gggcagcagc tggttcacac cataatcatc
     1441 atagtgggaa gctccggcca gctcccaggt tcggagcagc tcggtatcgt cctgacgcga
     1501 aatgcggtaa cggaacatga aaacatcctg ttccgtttcg agctgcagaa ttttgagcgc
     1561 attgtcggca cggatgtaga ccttcgatgg cgcaagtgcc gtaataccat ccttcagcgg
     1621 tgctgaaagg gggaatcgcg aatagagaag catcgcatcg aagacctgca cggccgccgc
     1681 ctgaaacgcg ttgacatagg tggtgagccg aatggccgac tgcgactcac cagccccaat
     1741 cacgcccgct accggaaggc cacccagcag ggattccgac tgcgaacgca ctgccagtgc
     1801 cgcctgggaa aaaacgtcgt atgacacacc atctgtggtc agacgcaact caccgtatcg
     1861 ttgccggtcc caggcgcgca gcgcccgcac acccagcgtt tgtgcgctga cgcccaccca
     1921 ggcgtaacct tcccgcatca gctcttcgtg ggactgcgcc cagatcacat cgacatccat
     1981 gatcgctgtg ttattcagcc actcgacaac cacgatgcca ttgaactttg ccggatccgc
     2041 tggccgcctg accagaagtc gtgttgcgta agggacattt gcgcgcactg tcttgacgtc
     2101 ccacttgcca tcgctgcgcc acttccccgc cctgccatag gtattggccg atccggacat
     2161 ctggaactca ctttcaacat aacccagcgc ggacaggtcc aggatcgacg ccacgaaggg
     2221 gtgttgtttt gatccagcgt cgtaggcaat ggatttgatg acaggttcag gtacaaccac
     2281 cgctgcatga acggggcaca tcaacaggaa gacgagaaga cacaggactc gcaaggccag
     2341 cgtgcggatg aatgcgctgg ccggattgac tgcgttcatt gactattcct tttgttttta
     2401 ttgtgttggc ctgttctatt tattggttct ggcgga
//
LOCUS       DASUEG010000869         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_298798, whole genome shotgun sequence.
ACCESSION   DASUEG010000869 DASUEG010000000
VERSION     DASUEG010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298798"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..798)
                     /locus_tag="VFX11_16370"
     CDS             complement(127..798)
                     /locus_tag="VFX11_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940251.1"
                     /translation="MLMRHVTFSLLLCSTPLATQAQGLEPLSDSEMSSAGAQQGVLLN
                     LTLLNNMTRNGSGALVRDSQCTESVQPNPCRMGLEIAQRDTWFMAKEFYGSLQIKDLR
                     MDAGFLPATATGYQNYNRFRDAAGNCLLSGNPSGCNPVGLPAIRTSYPSSADGTPSVY
                     DDVLSFMNVGRTWLEFTTTSPAVEGYMRDTTTNSAFGVRLSDSRALNEPAHMRFRGTA
                     YVFGF"
     gene            complement(792..2300)
                     /locus_tag="VFX11_16375"
     CDS             complement(792..2300)
                     /locus_tag="VFX11_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940252.1"
                     /translation="MRKSATSALLLALLAVGAALPLPSTARAALADLDDASLEEVSGA
                     GIAIALDDFEFAMAPTSYFEQVGSAPAGGGCSGTGSVASNINCWRRGDLRWYGINISA
                     AGGSNDGFHWNDSTACSSSSLNCPRGGVIDNFSPFDNPYIIRAWSPAGYAANGNCVNG
                     ATGGCTAPATPINKAIYEFLAPTSQPNYTVSWWGEMEVGSTRNSQTQALATNAGTVLK
                     SQNIIRGNAAGSVFRMFNYGTAANPTFAMFYHSYLRGDYRFSVTQSVVNDTIGQAPVF
                     ADTEGLYFRNVDAFLPIGQLYYQALTIKAIGTAGNFELSLTPLPTGTTTGDVAVQNKH
                     YSLNATDTSYGYETARANNTGGTTSADYKLTHGYVRWGSMDWNGATTGSTTTGRNAIN
                     EGNDGVLFRSCSGCSTFYAYADRPLVIDKRGPTGSTQRSQNYQCSTSNAGSCTIPTGA
                     GGSTNIVSGGAANSTRTYPVTATNAVNLGDSRIDGLMIQTLRIQSCGGSPTC"
     gene            complement(2529..2927)
                     /locus_tag="VFX11_16380"
     CDS             complement(2529..2927)
                     /locus_tag="VFX11_16380"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004816004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4940253.1"
                     /translation="MNDAPAPQGTISLQTIAMPADTNWNGDIFGGWLMAQMDLAGAVA
                     ARKRAHGRITTVAVESMVFHHPVAIGDVISCYTTIERIGTTSMKIRIEVWETADPCQP
                     TVKLTEGVYTYVAIDEKGRPRPVPADACPS"
     gene            complement(2979..>3210)
                     /locus_tag="VFX11_16385"
     CDS             complement(2979..>3210)
                     /locus_tag="VFX11_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4940254.1"
                     /translation="NPRTLQRRLKEENITFQSLFDSSRYRLACELLAQTGNPVEDIAA
                     MLGYSEPTNFYRAFKTWSGLTPNEYRRQYRRP"
BASE COUNT          576 a          923 c         1069 g          642 t
ORIGIN      
        1 ccgaagtcac tggcgacctg tgtggctatt gccgcatcca gtgcatcgcc agtgctggca
       61 tgcaggcaca ccggaaccat cagcaatgcc agcagcgcga tgtttcgcca ttgcatgaca
      121 atccgctcag aaaccgaaaa catatgcagt cccccggaaa cgcatgtggg cgggttcgtt
      181 gagtgcgcgc gaatcactca ggcgcacgcc gaaggcgctg ttggtggtgg tgtcacgcat
      241 atagccttca accgccggac tggtagtggt gaattccagc caggttcggc ccacgttcat
      301 gaaactcagc acgtcgtcat agacgctggg tgtgccatcg gcgctgctgg gataactggt
      361 ccgaatggcc ggcaggccga cgggattgca gccggaggga ttgccgctca acaggcagtt
      421 gccagcggca tcgcggaagc ggttgtagtt ctggtagccc gttgccgtcg cgggtaaaaa
      481 accggcatcc atgcgcaagt cctttatctg cagcgagccg tagaattcct tcgccatgaa
      541 ccaggtgtcg cgctgggcga tttcgaggcc catgcggcag gggttgggct gaacggactc
      601 cgtacactgg ctgtcgcgca ccagtgcgcc cgagccgttg cgcgtcatgt tgttgagtag
      661 tgtcaggttg agcagcacgc cttgctgcgc accggcggac gacatttcgc tgtcgctcag
      721 cggctccagc ccctgtgcct gtgttgccag aggcgtgctg cacagcagga gagagaaagt
      781 gacgtggcgc atcagcatgt tggcgagcct ccgcaactct ggatgcgcag cgtctggatc
      841 atcaggccgt cgatacggct gtcgcccagg ttgaccgcat tggtagcggt gaccgggtag
      901 gtgcgggtgc tgttcgccgc gccgccactg acaatgttgg tactgccacc ggcgccagtc
      961 ggaatcgtgc agctgccggc attgctggtg ctgcactggt aattctgcga acgctgcgtg
     1021 ctgccggtcg ggccgcgctt gtcgatgacc agtggccggt cggcataggc ataaaacgtg
     1081 gagcagccgg agcagctgcg gaacaggacg ccgtcattgc cctcgttgat ggcattgcgt
     1141 cccgtggtgg tcgagcccgt tgttgctcca ttccagtcca tcgatcccca gcgcacgtag
     1201 ccatgggtca gcttgtagtc agccgacgtg gtgcccccgg tgttgttggc gcgcgcggtt
     1261 tcatagccat aggaggtgtc agtggcgttc aggctgtagt gcttgttttg cacggccaca
     1321 tcaccggtgg tggtcccggt gggcagtggc gtcagcgaca gttcaaagtt gcccgccgtg
     1381 ccaatcgcct tgatcgtcag cgcctggtag tagagctggc caatcggaag gaaggcatcg
     1441 acgttgcgga aatacaaacc ttccgtatca gcaaaaacgg gtgcctgacc gatggtgtca
     1501 ttcaccaccg attgcgtgac actgaagcgg taatcgccac gcagatagct gtggtagaac
     1561 atcgcgaatg tcggatttgc cgccgtcccg taattgaaca tgcggaagac cgagccggcc
     1621 gcgttgccgc gaatgatgtt ctggcttttc agcacggtac cggcattggt ggccagcgcc
     1681 tgcgtttgcg agttgcgcgt actgccgact tccatttcac cccaccatga caccgtgtag
     1741 ttgggctgcg aggtgggggc caggaattcg tagatggcct tgttgatggg ggtggccggc
     1801 gcggtgcagc cgccggtagc gccgttgacg cagttgccgt tggcagcata gccggccgga
     1861 gaccaggcac ggatgatgta ggggttgtca aagggcgaga agttgtcgat caccccgcca
     1921 cgcggacagt tcagcgagct ggagctgcaa gctgtgctgt cgttccagtg gaagccgtca
     1981 ttgctgccac cggcagccga gatgttgatg ccataccagc gcagatcacc gcggcgccag
     2041 caattgatgt tggaggcgac cgatcccgtc cccgagcaac cgcccccggc cggggcagag
     2101 ccgacctgct cgaagtagct ggtcggcgcc atggcaaact cgaagtcatc cagggcaatg
     2161 gcgataccgg ccccggatac ctcttccagt gatgcatcgt ccaggtcggc aagcgcggcc
     2221 cgggcggtgg acggcagcgg caaagcggcg cccacggcca gaagggcaag cagaagcgcg
     2281 gaggtcgcag actttcgcat ggcagccagt gttattttta tgatggctgc acagtgtgct
     2341 ggctattttt tgatctgaca gcgtcgtgac gcgtcgtcca ctgtcaatgg gcgacaaggg
     2401 tgagcaggtg ctcaggccgg accttgcgag gctccggtct atcgcgatgt tggtggctgt
     2461 cccgtcatgg attccgcgac agggacccat ggcggtcggt gccggtgaca gccagagccg
     2521 gcaatggctc agctcggaca ggcatctgca ggcacgggcc gtggccgccc cttctcgtcg
     2581 attgccacat aggtatagac accctccgtc agcttcaccg tgggctggca ggggtccgcg
     2641 gtttcccaga cttcgatgcg gatcttcatc gaggtcgtgc cgatgcgctc aatagtggtg
     2701 tagcaggaga tgacatcgcc gatggccacc ggatgatgga acaccatgct ttccacggcc
     2761 accgtggtga tgcggccatg ggcgcgcttg cgtgcggcga ccgcgccggc caggtccatc
     2821 tgggccatca gccagccacc aaagatgtcg ccgttccagt tggtgtcggc cggcatggca
     2881 atcgtctgca gggaaatggt gccttgcggg gctggggcat cgttcatgaa gtgctcctct
     2941 tgaaggggga tgcccgcgtt ataacagaac tgaccgggct atggccgacg gtactggcgc
     3001 cggtattcat tcggcgtcag gcctgaccac gtcttgaacg cccggtagaa attggtgggc
     3061 tccgagtagc ccagcatggc ggcaatgtcc tcgaccggat tgccggtctg ggccagcagt
     3121 tcgcaggcca ggcggtagcg gctgctgtca aacagcgact ggaaggtgat gttctcttcc
     3181 ttcagtcggc gctgcagggt gcgcgggttc
//
LOCUS       DASUEG010000870         6714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_166546, whole genome shotgun sequence.
ACCESSION   DASUEG010000870 DASUEG010000000
VERSION     DASUEG010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166546"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFX11_16390"
     CDS             complement(<1..562)
                     /locus_tag="VFX11_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX4940255.1"
                     /translation="MSVYAKWWIFGPLIGLLTLASIRCELFENNLFDTYATGEWPTVT
                     CAPSDMIYRSADGRCNDLDLPAMGMAGMRMGRNIQPDYSWQDTDNLLNPNPRDVSKEL
                     LTRTDGIREVPFLNYLAASWIQFMIHDWFEHGNRDHSNPIAVPVDGDDTHNSGDGTMP
                     IGRTPADPTKNPSDGRPATFLNENTAW"
     gene            1036..3366
                     /locus_tag="VFX11_16395"
     CDS             1036..3366
                     /locus_tag="VFX11_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4940256.1"
                     /translation="MNTIKKSLWIGMDDSAPVLSACAGQTGRHLSHDAWVAELGWSQQ
                     RLTLVCAPSGSGKSRLLGELQLMLSDNSTAVDCIRIDLADGGDAAVTLTQIAQQLTAF
                     APSSREKYRQQSLSAPALVSAISRQLPGCARPLRIFVDGLVAGRDDSLLYWLLQPGLS
                     QAVDVRWFVAMDGMPDAALAALLSAAQARLVDAAELMIGASTLPQVASPASFGWLVES
                     MGLPSSLRTPSALTLAGRLCRRWMQQHDARAVLQLLAPVPADEFLSHANTALPLLWAL
                     IIELRLDEAAELLKKIRPEALLQDLSLPFTEIDNTLEACSLLIRLAGDESLAAGQRTT
                     LLGRITEKTSPLQTELLNRHAALLYGGGHIPLAKAVARRSLHIAQQQDDVIQLQLSRT
                     QMLLCDFVQGHLDAVVEEVEQDAAQLSRRLCALAPGMTDPALDLAIAINNCTRAYLYY
                     ECGRVADAEPLLAPALARIAPCHFSLPLTLATITRARILLRSGQPLAAEQMLEQLDEM
                     VEIQRNRRLRAFVCFEKMCQRLAAGVRADDLIARYELDAQPVHVETLFRDQFQEEHLF
                     WLKSRLVALLMNADFATASEYALKGVIKSLNIGSFRHLATFYLFKALSEFRLGRYQDA
                     KATGNRALDLCWQHGYRQLLVDQARTMQDLWQQLQRQGDMSSLPDADFLRGLLTRAEP
                     GRAILRPAGAALPARDIGAELRRDLGLTDKEVEILTLLAEGLCNKKIGSRASIALTTV
                     KWHLQNIFSKLDVRNRTEAVLKAQDLGLLESGSPAR"
     gene            complement(3476..4249)
                     /locus_tag="VFX11_16400"
     CDS             complement(3476..4249)
                     /locus_tag="VFX11_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940257.1"
                     /translation="MMRYKLVAAMLLPTLLLTGCGGGGETRRSTSNSANLTGLWRMQL
                     TTAQNSMTAESNVSFHITESASGLNMVACDNRDQVKLQKAATGIDGLPVGTMKIVNND
                     TMTAKNELGTSKATKMATTAKFDMGTLQLSASQLGTQNFTDLCVQSSDARVLGVATQD
                     NYSATTLYNGKPLLFDILVIGNLGVGTYKLSSTPAVGEATLRLQGDGLKTPLNRTEMS
                     FEKGTLTITDDTAVWLKGNFSATMPNGSPLTGSFSFEKP"
     gene            complement(4468..4845)
                     /locus_tag="VFX11_16405"
     CDS             complement(4468..4845)
                     /locus_tag="VFX11_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX4940258.1"
                     /translation="MTSLYERIGGDAAVNAAVDLFYVKVLADKRISHFFEGVDMAKQH
                     RMQKGFFTFAFGGPANYSGRGMEAAHRKLVQEKGLNDSHYDAVMENLGATLQELGVPA
                     DLIAEAAAIAESVRGPVLGRAAD"
     gene            complement(5097..>6714)
                     /locus_tag="VFX11_16410"
     CDS             complement(5097..>6714)
                     /locus_tag="VFX11_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940259.1"
                     /translation="RGREIFWSYDFNIGLAHAVRNLNWKNIFEYKTTDPITREVADPA
                     KLIGAYPTVAGEVPAEAINDVYFNPYPQPNPIPGFLTVQPQLTKGNTKNSGWVGYRYA
                     EASHPYTGPGQWIGFNCASCHSYRVSYEKGASNITKVIPGLPNPEWSMKWAVLGNKTG
                     DTTATFDGIEANEEGPSWDPGKVAVDKTALIYFMPAGTGEHNIVRLSDEGSETDNDYQ
                     FSPIAIPNITNYMAIRRSLSHTESYVGFEGSYIHAEEPDGATGSMLASELKALTAYMT
                     VLDKDDNDLRNVGLYRWLKHNGKLGTQTGNGSLAEGSFVQNGWQSYPGVVSAVNAGKA
                     IFNRDCGSCHNDTLGAHTSEKMFRLDEVGRFFTPTIYHKDQQSIRATFLRDVYWTQHR
                     GLLTDGHVRNMEDLVNPDRCEEGTDLYNQYYTLHPPASPALGGPDFPEPYPAANRKGD
                     VFRVLKSPSTSETDTGAKRNRFIERHKYFVTVPWDDQYYYWDYQKMRAEWGPDELGTP
                     APVNLPAAPHPWCAQNASDVGNLVQYVLTL"
BASE COUNT         1209 a         1822 c         2233 g         1450 t
ORIGIN      
        1 accatgcagt gttttcgttc aggaaggtgg cggggcggcc atcgctgggg ttcttggtgg
       61 gatcagccgg cgtgcggccg atcggcatgg tgccatcacc gctgttgtgg gtgtcgtcgc
      121 catccaccgg cacggcgatc ggattgctgt gatcgcggtt gccatgctca aaccagtcgt
      181 gaatcatgaa ctggatccat gaagccgcca ggtagttcag gaacggtact tcacggatgc
      241 cgtcggtacg ggtcagcagc tctttgctga catcgcgcgg attggggttg agcagattat
      301 cagtgtcctg ccagctgtaa tccggctgga tattgcggcc catgcgcatg cctgccatgc
      361 ccattgccgg cagatccaga tcgttacagc ggccatcggc gctgcggtag atcatgtcgc
      421 tgggggcgca ggtcacagtc ggccattcac cggtggcgta ggtgtcgaac aaattgttct
      481 cgaacagctc gcagcgaatg ctggccagcg ttaacaatcc aataagtgga ccaaaaatcc
      541 accatttagc gtatacactc atagttgctc cactcaggtt ttttataatt attgaaacgc
      601 gttgccagag aaccacgtaa acgtgctctc aatccgctag agcataatga gttggaatag
      661 tggcatcgcc gtacataggt aggggtgaga cagggagggg ggagggaggg ggagcgggcc
      721 ccgagccaac acaaatggac ttgtgttacc ccggaaaaaa tggtgtgctg tgaacgcgtt
      781 ttgccggaag ggaaattatt gcagtgcggg tcgcggcagg tgcagggatt gcggctgaca
      841 atatggcaat caccaagaaa aagagactcc gcgcacaaat cctgacaata aaataaaact
      901 taaaaaatca gagtcaggat taaatcaaac gtcttaagcg ttggcaatag accctggttt
      961 cattttttga cctttttcgg gacgaaaggg aagaccgcac agaataacaa taatcgtcag
     1021 cgcgaaatcg ggatcatgaa taccatcaag aagtcactgt ggattggcat ggatgattcg
     1081 gcaccggtgt tgtctgcctg tgccggtcaa accgggcggc acttgtcgca cgatgcatgg
     1141 gtcgcggaac tggggtggtc gcagcagcgg ttaaccctcg tctgcgcgcc gtcaggctcc
     1201 ggcaaaagcc ggctgctggg tgagttgcaa ttgatgctgt ctgacaactc gacagcagtg
     1261 gattgcatcc gtatcgacct ggcggatggc ggagatgccg cggtaaccct tacccagatt
     1321 gcacaacagc tcacggcgtt cgctccttcc tcccgcgaaa aataccgaca gcaaagcctg
     1381 agtgcacccg cgctggtatc ggccatcagt cgccagttgc ccgggtgcgc gaggccgctg
     1441 cggatttttg tcgatggtct ggtggccggg cgtgatgaca gtttgctgta ctggttactg
     1501 cagccgggcc tgtcgcaggc cgtggatgtg cgctggtttg tggcgatgga cggaatgccg
     1561 gacgcggcgc tggcggcatt gttgtccgcg gcgcaggcca ggctggtgga tgccgccgag
     1621 ttgatgatcg gagccagtac gctgccgcag gtggccagcc cggcctcatt tggctggctg
     1681 gtggagtcaa tggggcttcc atcctcgttg cggacgccat ccgccctgac actggccgga
     1741 cggttgtgca gacgctggat gcaacagcac gatgcccgtg cagttctgca attgctggcg
     1801 cctgtgccag cggacgaatt cctttcccat gccaatactg cattgcccct gctgtgggcg
     1861 ctgatcatcg agttgcggct ggatgaggct gcggagctgc tgaagaaaat ccgccctgag
     1921 gcgctgctgc aggatctgtc cctgcccttt acggaaatcg ataacacgct ggaggcctgc
     1981 agcctgctga tccggctggc cggcgacgag tccctggcgg ccgggcagcg cacgacgttg
     2041 ctgggcagaa tcaccgagaa aacctctccg ttgcagaccg aattgctcaa ccgccacgcg
     2101 gcgctgctgt atggcggtgg ccacatcccg ctggcaaagg cggtggcgcg acgcagcctg
     2161 catatcgcgc agcagcagga tgatgtgatc cagctgcagt tgtcccgcac gcaaatgctg
     2221 ttgtgtgatt ttgtgcaggg tcacctcgat gccgtggtgg aggaagttga acaggatgcc
     2281 gcgcagctga gccggcgtct gtgtgcactg gccccgggga tgaccgatcc agcgctggat
     2341 cttgccattg ccatcaacaa ttgcacccgc gcctacctgt actatgagtg cggccgggtg
     2401 gcggatgccg aaccgctgct ggcaccggcg ctggcgcgta tagccccgtg ccatttctcg
     2461 ctgccgctga cactggccac catcacgcgc gcgcggattc tgctgcggtc cggccagccg
     2521 ctggcggcgg aacagatgct ggagcagctg gacgaaatgg tggaaatcca gcgcaaccgg
     2581 cggctgcggg cattcgtgtg tttcgagaaa atgtgccagc gcctggccgc cggtgttcga
     2641 gccgacgacc tgattgcacg ctatgaactg gatgcacagc cggtgcacgt ggaaaccctg
     2701 tttcgcgatc agttccagga agagcatttg ttctggctca agagccggct ggttgcattg
     2761 ctgatgaacg cggattttgc aaccgcgtcg gaatatgcgc tgaaaggcgt gatcaagagt
     2821 ctgaacatcg gctcattccg tcacctcgcc actttttatc tgttcaaggc gctgagtgaa
     2881 ttccgcctcg gccgctacca ggacgccaag gccaccggca atcgcgcgct ggatctgtgc
     2941 tggcagcacg gttaccgcca gttgctggtg gatcaggccc gcaccatgca ggacttgtgg
     3001 cagcagttgc aacgccaggg tgatatgtcg tcattaccgg atgcggattt tctgcgtggg
     3061 ttgctgaccc gagccgagcc gggccgggcc atactgcgac cggccggcgc agcgttgccg
     3121 gcgcgcgata ttggcgcgga gttgcggcgc gatctgggcc tgaccgacaa ggaagtggaa
     3181 attctcacgt tgctggcgga aggtttgtgc aacaagaaaa tcggcagtcg cgccagcatc
     3241 gcgttgacca ccgtgaaatg gcatctgcag aacattttca gcaagctgga tgtgcgcaat
     3301 cgtaccgagg ccgtgctgaa agcgcaggat ctggggctgc tggaaagcgg gagccctgcg
     3361 cgctgagttc agggcgcgat gggattcttc ttgcgatcgg tgattctgaa atgcgaaagg
     3421 ccggcaccgg tgagggtgtc ggcctttttt ggattcgcca ggaatcccgc tgcgatcagg
     3481 gtttctcgaa ggagaagctg ccggtcagcg gggagccgtt cggcatcgtg gcgctgaagt
     3541 tgcccttgag ccataccgca gtgtcgtcgg tgatggtcag ggtgcctttc tcgaagctca
     3601 tttcagtgcg gttcagcggg gttttcaggc catcaccctg caggcgcagt gtggcttcac
     3661 ccaccgccgg cgtgctggac agcttgtagg tgccaacacc cagattaccg attaccagga
     3721 tgtcaaacag caggggcttg ccgttgtaca gggtagtggc ggaatagttg tcctgggtgg
     3781 ctacgcccag cacgcgggca tcggacgatt gcacgcacag gtcggtgaag ttctgcgtac
     3841 ccagctggct ggcagacagc tgcagggtgc ccatgtcgaa cttggcggtg gtggccatct
     3901 tggtggcttt cgaggtgccc agttcgttct tggcggtcat ggtgtcgttg ttgacgattt
     3961 tcatggtgcc caccggcaga ccatcgatac cggttgctgc cttctgcagt ttcacctggt
     4021 cgcggttgtc gcaggcgacc atgttcagcc cgctggcgct ttctgtgatg tggaagctga
     4081 cgttggattc agcggtcatg ctgttttggg cggtggtcag ctgcatgcgc cacaaaccgg
     4141 tcagattggc actgttgctg gtggagcggc gggtttcgcc gccgccgcca cagccggtta
     4201 gcaggagggt gggaagcagc atggcggcaa cgagtttgta acgcatcata ggactaatac
     4261 ccttatctgg aatttccggt ttgttgtttt tattgtcatt gccggcatgg ctggtcccgg
     4321 gcaatggttt cgcgattgaa cgtctacggt cacgacatta aacccgatcg ccggaagctc
     4381 agcaccatac aaaggtaggg gagggtggag ggggtgggac ggtgccggcg gcgccccagg
     4441 ccgccggcac tggaatggca ccggatttca gtcggccgcg cgtcccagga cggggccgcg
     4501 cacgctttcg gcgatggcgg cggcttcggc gatcaggtcg gcgggcacgc ccagttcctg
     4561 cagggtggcg cccaggtttt ccatcacggc gtcgtaatgg ctgtcgttaa gtcccttttc
     4621 ctgcaccagc ttgcggtggg ccgcttccat gccgcgaccg gagtagttgg ccgggccacc
     4681 gaaggcaaag gtgaaaaagc ccttctgcat gcggtgttgc ttggccatgt ccacgccctc
     4741 aaagaaatgg ctgatccgct tgtcagccag cacctttaca tagaacaggt cgacggcggc
     4801 gttgacggct gcgtcgccgc cgatgcgttc gtacagtgat gtcatggtgt ttcccctcta
     4861 caggataaga atgccccggt gggcaggttg ggtctgaagc tgggtgccag gcagacaccc
     4921 gccagccatg ttacagggtc gggaagggta ttgcgcctat gtcattgggg gtaggttgcg
     4981 tagtcaccag tatcggacag gggcaacggg gcctgtttga tgcgtgaatt taaagtgatg
     5041 accagaaaaa cggggccctt gggccccgtt ggttggatga agagcgataa tgcaccttac
     5101 agcgtcaaca cgtactgcac caggttgccc acgtcagacg cattctgtgc acaccagggg
     5161 tgcggtgcgg ccggcaggtt taccggtgcc ggtgtgccca gttcatccgg gccccactcg
     5221 gcccgcattt tctggtaatc ccagtagtag tactggtcgt cccagggcac ggtgacgaaa
     5281 tacttgtgcc gttcgatgaa gcggttgcgc ttggcgccgg tgtcggtttc gctggtggac
     5341 ggcgatttca gcacacggaa cacatcgccc ttgcggttgg cggcgggata cggctcgggg
     5401 aagtccgggc cgcccagtgc cggactggcg ggcgggtgca gggtgtagta ctggttgtac
     5461 aggtcggtgc cttcttcgca gcggtccggg ttgaccaggt cttccatgtt gcgcacgtgg
     5521 ccgtcggtga gcaggccgcg atgctgggtc cagtacacgt cacgcaggaa ggtggcgcgg
     5581 atcgactgct ggtccttgtg gtagatggtg ggagtgaaga agcggcccac ttcgtccagc
     5641 cggaacatct tctcgctggt gtgcgcgccc agggtgtcgt tgtggcagct gccgcagtcg
     5701 cggttgaaga tcgctttgcc cgcgttcacg gcgctgacca cgccggggta gctctgccag
     5761 ccgttctgca cgaagctgcc ttcggccagg ctgccgttgc cggtttgcgt gcccagcttg
     5821 ccgttgtgtt tcagccagcg gtacaggccc acgttgcgca ggtcgttgtc gtccttgtcc
     5881 agcacggtca tgtacgcggt cagcgctttc agttccgacg ccagcatgga accggtggcg
     5941 ccatcgggtt cttccgcgtg aatgtacgag ccttcgaagc cgacgtagct ttcggtgtgc
     6001 gacagcgagc ggcggatcgc catgtagtta gtgatgttgg gaatggcgat gggcgagaac
     6061 tggtagtcgt tgtcggtctc gctgccttca tccgacaggc gcacgatgtt gtgttcgccg
     6121 gtacccgctg gcatgaagta gatcagcgcg gtcttgtcga cggccacttt gcccgggtcc
     6181 cagctggggc cttcctcgtt ggcttcgatg ccgtcgaagg tggcggtggt gtcgccggtc
     6241 ttgttgccca gcacagccca tttcatggac cactcggggt tgggcaggcc ggggataacc
     6301 ttggtaatgt tgctggcgcc tttctcatag gaaacgcgat aagagtggca ggacgcgcag
     6361 ttgaagccga tccactggcc ggggccggtg tagggatggc tggcctcggc gtagcggtaa
     6421 cccacccagc cgctgttttt ggtgttgccc ttggtgagct ggggctgcac ggtgaggaag
     6481 ccgggaatcg ggttgggctg cgggtagggg ttgaagtaca cgtcattgat ggcttccgcc
     6541 ggtacttcgc ctgccacggt ggggtaggcc ccgatcagct tggccgggtc tgccacttcg
     6601 cgggtgatgg ggtcggtggt cttgtattcg aagatgttct tccagttcag gttacgcacg
     6661 gcgtgggcca ggccgatgtt gaaatcgtag gaccagaaga tctcgcggcc gcgc
//
LOCUS       DASUEG010000871         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1934694, whole genome shotgun sequence.
ACCESSION   DASUEG010000871 DASUEG010000000
VERSION     DASUEG010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934694"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1740
                     /locus_tag="VFX11_16415"
     CDS             202..1740
                     /locus_tag="VFX11_16415"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX4940260.1"
                     /translation="MTAKVITACGEISGIQRENHVEFRGIPYAEQPIGVSRFKAPQPL
                     LPDEEPMAAETFGDACQQEEVSMFGISQMSEDCLYLNIWTPDCDNRKRPVMVWIHGGG
                     YQTGSGAQLIYRGGQLAVHGDVVVVTLNYRLGAFGFLYLNDLIPEHYNVGANNGLLDQ
                     IEALKWIRQNIAQFGGDPDQVTLFGESAGAMAIATLLACPAAQGLFKRAILQSGSADQ
                     VLTREEANKIAHRFLEVTDINPAKPDKLWQLTPQQIVKAQRDCMRMSFDRGMYAQQVP
                     QTGMTLVPVIDGLTLPQSPLRSLEQGDARHIPLLVAWTRDEWNLFLRMPGAEKIMNNE
                     TFNNLDKLGLIKLCEKNLPGMGERAANLYEKILKARNPNASYTDMFSAFESDRVFRIP
                     SLRIGELQGRHSQDVFLCQFNWDKGLLGACHASDIPLVFGCADNGVGQFLTGGGDGAR
                     RLSDIVQSSWIAFARSGNPATDRVGHWPAFNKDTRKVMCFDETTQLQDDPLEQEQELW
                     EGIL"
     gene            1908..>2944
                     /locus_tag="VFX11_16420"
     CDS             1908..>2944
                     /locus_tag="VFX11_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX4940261.1"
                     /translation="MSKQVFFIGQRWANTAETDLGIGIVTQVNDRTISLFFPAAEELR
                     NYAKANAPLTRLQFHPRDTIETQDGTRYRVLSRTEQDGLFFYESEGENGEIKTLCETQ
                     VQGSSQMPEALKRLLLGQLDDFRNFGLRLRTWELLANYDRHPAKGFMGARVDLIPHQF
                     HIAQTVSARTAPRVMLADEVGLGKTIEAGLIAHQQLISGKIGRVLILVPDSLVHQWLV
                     EMRRRFNLPVRVLDQDQCEALCEQEQTDNPFLYEQLVLCSIQFLLHHKQWAEAALDTP
                     WDLLVVDEAHHLAWEPGNASPAYSLVEQFSLRAKGLLLLTATPEKEGPESHFAQLHLL
                     DPLRYAELPAF"
BASE COUNT          613 a          955 c          851 g          525 t
ORIGIN      
        1 catgaatgat ctcgacagct ggttgtgaat tgaactgctt ttgattctgg accaaacgtt
       61 cttgaatgca aaaggccgga agcgtcattt ttcgggcaga tattgtcgcc ccgtgctggc
      121 cgtggcaatc ttccctgaat atcaggtttt gaacccgttc acacatccca ccgcctcaac
      181 gttaacgaca aggaatcctc catgacggcc aaagtgatca ccgcctgtgg cgaaatcagc
      241 ggcatccagc gcgaaaacca tgtggaattc cgtggcattc cctatgccga gcaaccgatc
      301 ggtgtaagcc ggttcaaggc gccgcagccg ctgctgccgg acgaagaacc catggcggca
      361 gaaaccttcg gcgacgcctg ccagcaggaa gaagtgtcca tgttcggcat cagccagatg
      421 agcgaggact gtctttacct gaacatctgg accccggatt gcgacaaccg caagcgtccg
      481 gtaatggtgt ggattcacgg cggcggctac cagaccggct ccggcgcgca gctgatctat
      541 cgcggcggcc agctggcggt gcacggcgac gtggtggtgg tgacgctgaa ttaccggctc
      601 ggtgcgttcg gcttcctcta cctgaacgac ctgattcccg aacactacaa tgtcggcgcc
      661 aacaatggtc tgctggacca gatcgaagca ctgaaatgga ttcgccagaa cattgcccag
      721 ttcggcggcg atccggacca ggtgacgctg ttcggcgagt ccgccggcgc catggccatc
      781 gccacgctgc tggcctgtcc tgcggcccag ggtctgttca aacgcgcaat cctgcagagc
      841 gggtccgccg accaggtgct cacccgcgag gaagccaaca aaattgccca ccgctttctg
      901 gaagtcaccg acatcaatcc cgccaagccg gacaagctgt ggcaactcac gccgcagcag
      961 atcgtcaagg cccagcgcga ctgcatgcgc atgagtttcg atcgcggcat gtatgcacag
     1021 caggtaccgc agaccggcat gaccctggtg ccagtgattg atggcctcac cttgccgcaa
     1081 tcgccgctgc gctcgctgga gcagggcgat gcccgtcaca ttcccctgct ggtggcctgg
     1141 acccgcgatg aatggaacct gttcctgcgc atgcccggcg cggaaaaaat catgaacaac
     1201 gaaaccttca ataatctcga caagctgggc ctgatcaaac tgtgcgaaaa gaacctgcca
     1261 ggcatgggcg aacgcgccgc caacctgtat gaaaaaatcc tgaaggcccg caatcccaac
     1321 gcttcctaca ccgacatgtt cagcgcgttc gagagcgacc gcgtgttccg cattccgtcg
     1381 ctgcgcatcg gcgaactgca gggccgccac agccaggacg tcttcctctg ccagttcaac
     1441 tgggacaagg gcctgctggg cgcctgccac gccagcgaca ttcccctggt gttcggctgc
     1501 gccgacaacg gcgtaggcca gttcctcacc ggtggcggcg atggcgccag aagactcagc
     1561 gatatcgtgc agagcagctg gatcgctttt gcgcgctccg gtaatcccgc taccgaccgg
     1621 gtcggccact ggcccgcctt caacaaggac acccgcaagg tgatgtgttt cgacgagacc
     1681 acccagctgc aggacgatcc gctggagcag gaacaggagt tgtgggaagg gattttgtaa
     1741 tctgatccgg gccggccatg cgttgatcca atcccagcaa cctgcgcagg aacccggaca
     1801 ttacggcatc tgcgcggggt ggatgcgtcg ttaatttttt gttcatggat taataacggc
     1861 gcatttccta gaatacgcgg cttcagagtc gcggggtcaa tcgtcacatg agcaagcagg
     1921 tattcttcat cggacagcgc tgggccaaca ccgccgaaac ggatctcggc attggcatcg
     1981 tcacccaggt taacgaccgc accatcagcc tgttcttccc cgccgccgag gaactgcgca
     2041 actacgccaa ggccaacgcc cccctcaccc gcctgcagtt ccacccccgc gacaccatcg
     2101 aaacccagga cggcacccgc taccgggtgc tatcccgcac cgagcaggac ggcctgttct
     2161 tttatgaatc cgaaggcgaa aacggcgaga tcaaaaccct gtgcgaaacc caggtgcagg
     2221 gcagcagcca gatgcccgag gcgctaaagc gcctgctgct gggccagctc gacgacttcc
     2281 gcaacttcgg cctgcgcctg agaacctggg aactgctggc caactacgac cgccaccctg
     2341 ccaagggttt catgggcgcc cgggtggacc tgattccgca ccagttccac atcgcccaga
     2401 ccgtgtcggc ccgcaccgct ccccgcgtca tgctggcgga cgaagtgggc ctgggcaaaa
     2461 ccatcgaagc cggtctgatc gcccaccagc aactgatcag cggcaagatc ggccgggtgc
     2521 tgatcctggt gccggactcc ctggtgcacc agtggctggt ggaaatgcgc cgccgtttca
     2581 acctgccggt gcgggtgctg gatcaggacc agtgcgaggc cctgtgcgag caggaacaga
     2641 ccgacaatcc cttcctgtac gaacagctgg tgctctgctc gatccagttt ttgctgcatc
     2701 acaaacagtg ggccgaagcc gcgctggaca ccccctggga tctgctggtg gtggacgaag
     2761 cccatcacct ggcctgggaa ccgggcaacg ccagcccggc ctactcgctg gtggaacagt
     2821 tctcgctgcg ggcgaagggc ctgctgctgc tgaccgccac gccggagaaa gaaggccccg
     2881 aaagccactt cgcccagctg cacctgctgg acccgctgcg ctacgccgaa ctgccagcct
     2941 tcat
//
LOCUS       DASUEG010000872         7330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1690817, whole genome shotgun sequence.
ACCESSION   DASUEG010000872 DASUEG010000000
VERSION     DASUEG010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690817"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1308)
                     /locus_tag="VFX11_16425"
                     /pseudo
     CDS             complement(74..1308)
                     /locus_tag="VFX11_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
     gene            1426..2001
                     /locus_tag="VFX11_16430"
     CDS             1426..2001
                     /locus_tag="VFX11_16430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4940262.1"
                     /translation="MSDIERRDYYRMEDRVHLIRTPLEKHLISDDPYGDQYNIPRQAL
                     LISQLQAIDNDSRDLLRQVGDYNRALGSYLRAMDEKIELLAKYVVSHDKELAQKEPVN
                     LSEGGISYYDGSPLAVGSFLHLIIVLFPSYSSIAAIGQVRTCEKIEEQPVIYRVGVEF
                     QVLLEADRKLIVRHIRRRQSQAIREQGQKSD"
     gene            2131..2415
                     /locus_tag="VFX11_16435"
     CDS             2131..2415
                     /locus_tag="VFX11_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940263.1"
                     /translation="MKTMMKSLIVMVLAGLCGNLLAEEIAVPAGQQGDSSVDRPRTGL
                     SMDQVTAKFGAPDQQLPAVGNPPITRWVYAGYTVYFENDRVIHSVLNAAP"
     gene            2484..3542
                     /locus_tag="VFX11_16440"
     CDS             2484..3542
                     /locus_tag="VFX11_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX4940264.1"
                     /translation="MTERFSLLPEWAEQEAVLMAWPHESTDWQPWLAAIQHDYVELAV
                     AIAREVPPVILCQDEAHRDLILRQLAGRCEQAPVLVIAPYNDTWCRDYGPITLGPITL
                     GPAGNTGQRRLLDFCFNGWGDKYDAELDNGINRSIAQVWKPELQTIDFELEGGSIETD
                     GAGTLLTTTHCLLDSNRNNDFNKEQIEAFVLEKLGLQRCLWLSRGALFGDDTDSHIDN
                     LARFCSADTIAFATCSDVNDPHHEHLQAMEAELQQFRQPNGRPYVLKAIEIPQPQFDE
                     NGKRLPGSYINFLIVNNSVIVPVFDCPQDQAALQTLQQCFPGKRLLPVPGKNLIKQYG
                     GPHCATMQLPKGTTLINR"
     gene            3574..4491
                     /locus_tag="VFX11_16445"
     CDS             3574..4491
                     /locus_tag="VFX11_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX4940265.1"
                     /translation="MSNKTLRVGVIQQPAWPDKAKSLAETERLLKQLVDAGKPELVLL
                     QELHCTHYFCQVEDTALFDLAEPLDGPSVQTLSRWAKQYNTVLVGSIFEKRAPGVFHN
                     TAAVFEKDGSLAGTYRKMHIPDDPGFYEKFYFTPGDAVKLNGDSGFTPIPTSVGNLGV
                     LVCWDQWYPEGARLMAMAGADLLLYPTAIGWDPRDTDDAQAKQKNAWQLIQRSHAVAN
                     HLPVIVANRVGHEDDPSGQSDGILFWGGSFISGQQGDILCEGDAHTAQYLIAEVELGR
                     TEYLRRMWPYFRDRRIDAYGGLTQRFLKP"
     gene            4551..5285
                     /locus_tag="VFX11_16450"
     CDS             4551..5285
                     /locus_tag="VFX11_16450"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4940266.1"
                     /translation="MLIIGHRGARREAPENTLAGFRYLRSLGVHHVELDVRLSKDQQL
                     VVIHDDTLNRTTAHNGNVRDFTAAELAQVNAAAKYAGGWPEATPVPLLRDVLKEWDDL
                     ESIQLEVKTTDENDLRTIAHLIRDLVDELDLIDTAIVTSMDTHLLGYMHQTAPEIRRG
                     YVAERFTREPLAMCQLHHCTHLITNYHRVTPGLVQTAHTIGLEVSVWTVNDLNIAHRI
                     QHCGADSIITDVPSLMLNNLRHDIEY"
     gene            5269..6318
                     /locus_tag="VFX11_16455"
     CDS             5269..6318
                     /locus_tag="VFX11_16455"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX4940267.1"
                     /translation="MTSNTKRLLTTLLAGAATLLQGCDASFYWQAARGQFDLLQRRQD
                     IPALLQSDSTDANLKRQLEYVLQVRTFAAEQLHLTVNDNYLTYVDLQRPFVVWNVFAT
                     PELSLENRRWCFPVAGCVPYRGYFAETDAQAYAGTLAQQGFDTYVGGVAAYSTLGWFD
                     DAVLNTFVSRDQLSLAALLIHELAHQTLYVPDDSTFNESFATAVENLGLERWIEQAGL
                     QDQLPAYREAKQRQQQFLDLVLTHRDEREKLFASSLTDAEKRQRKQELQAALQLSYLQ
                     LKQSWNGYDGYDRWFSRPVNNAQLSTVATYHALEPGFRALFEQSGHHFPAFFQRCREI
                     ARLPKDQRDALLQRR"
     gene            6452..>7330
                     /locus_tag="VFX11_16460"
     CDS             6452..>7330
                     /locus_tag="VFX11_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4940268.1"
                     /translation="MSMPVSDKCEPFIHLDKLIPVLSDLYTAIKSRNEKCQEMLQGLI
                     SRINRIADTHPDATIAAIHLSKQQSPLKQPIYGAILAHLFSQQHGLDDKKEQALAQAV
                     LTCNLTFYEFQVLLNSMDGKLTDAQRAKLQKHPLQAAQYMEAAGFIDPQLTKAIRQHH
                     ERPDGSGYPNHLKGPDISDLALVVSMCEVYTARIDNRAYRKPVLAREALSHLYKEDDP
                     RLKGLLLSFAKAVGVYPPGTWVKLANNETAMVTRRQKNSPVPIVKALFDRNDIPYMGP
                     LTRDCNDPALKVVGATF"
BASE COUNT         1604 a         2348 c         2051 g         1327 t
ORIGIN      
        1 ggatttgagg acctggacct gctccgggcc ctcatcgaaa tgtttcacca gatcgcggca
       61 ttccagcacg gcattattca tagcgcaggg cctccgctgg ttgcactttt gacgctttca
      121 gcgccgggta aagggtggca aggaaactca tcaggaagcc catgccggca atgaacacca
      181 cgttttccca gcgcagttcc gatggcaggt aattcacaaa gtactgggcc atcagcttgg
      241 tgtgggtaac cttctccagc cagttgaaaa agtcgctgat gttgagggcg atggcaatgc
      301 ccagcgccat ccccgccagg gtgccaaaca cgccgatcac gcaaccctgc accatgaaca
      361 cacccatgat ggtgcccggc gaggcaccca gggtacgcag gatcgcgatg tccgctttct
      421 tgtccgtgac caccatcacc aggctcgaca caatgttgaa cgccgccacc gccacgatca
      481 gcatcagcag cagggccatc atggtttttt ccatcttgat ggcgttgaac agggtaccgt
      541 gggtgcgggt ccagtcagag gcgtaatact tgccctgctc ggcattggcc agatcccagg
      601 aaatggcgcc cgcctggaac agatcctcac ccgcagccga acaccttcca cttcgccttt
      661 cattctggcc agccgggcgg cgtcttcgat gtgaacgaag cccaccatgc cgtccagctc
      721 ggcgcccaca tggaacacgc cgatcagggt gaaacgcttg aaacggggca cgatgccggc
      781 gggcgtgatc gccgcctcgg gcaataccag ggttaccttg tcacccagcg ccagacccag
      841 cttgcgcgcc agggcgctgc ccagaatgat attgaagtcg ccaggtttca gatcgttcag
      901 gctgcccgca tccatgtgct ggggcaggat cgacacatcc ttttcatagt ccggctcgac
      961 cccggtaatg aaggcactgt ccaccacgcc cttgttgctg agcaggccct gcagctggat
     1021 gaacggggcg gccgcctcca ctccctcatg ggcgcgggct ttctcaatca gtgcctgcca
     1081 gtcgtcgaac ggccggtaac ccagcacgct ggcctggggc accatgccca ggatgcggcg
     1141 ctgcagttcg cggtcaaagc cgttcatgac cgacagcacc acgatcagca cggccacgcc
     1201 cagggtgagc cccaggatcg acgtcagcga tatgaaggaa atgaaatgat tacgccgctt
     1261 ggcgcgggtg taacgtaaac cgataaaaag ggggacaggt ctgtacatgg cgcgcattaa
     1321 accacagcaa tcaagcgaat acgaatacta taaggacagt ccagcaacat gggattcccg
     1381 ttttctgact acaattgtgg gatgaaaccg gaggatacag cccccatgtc cgacatcgaa
     1441 cggcgcgact attaccgcat ggaagatcgg gttcacctga tcaggacacc actggaaaag
     1501 cacctgattt cagatgaccc ctacggcgac cagtacaaca tcccccgcca ggccctgctg
     1561 atcagccagt tgcaggccat agacaacgac agccgggact tgttgcgcca ggtcggcgat
     1621 tataaccgcg ccctgggcag ttacctgcgg gcaatggatg aaaaaatcga gctgctggcc
     1681 aaatacgtgg tgagtcacga caaggagctg gcccagaagg agccggtgaa cctgagcgaa
     1741 ggcggcattt cctattacga cggctcgccg ctggcggtgg ggtcgttcct gcacctgatc
     1801 attgtcctct tcccgtccta ctccagcatc gctgccatcg gccaggtgcg cacgtgcgag
     1861 aaaatcgagg aacagcctgt catctaccgg gtcggcgtgg aattccaggt attgctggag
     1921 gcggaccgca agctgatcgt gcgccacatc cgccgccgcc agtcgcaggc aattcgtgaa
     1981 cagggccaaa aatcggactg aatgagcagc gataacaagg ttttgtctgc catttctgct
     2041 ataaataatc tgctgctata tcgaaacggt tgcaatgaat aggtttctat tactaatcga
     2101 aaaacaagct gcggctaaaa aggtcggacc atgaaaacaa tgatgaaatc cctgatcgtg
     2161 atggtattgg ccgggttgtg cggcaacctg ctcgccgagg agattgcggt acctgctggc
     2221 cagcagggcg acagcagcgt cgaccgtccc cgcaccggcc tcagcatgga ccaggtcacc
     2281 gccaaattcg gcgccccgga ccagcaactg cccgccgtag gcaatccacc gattacccgc
     2341 tgggtgtacg ccggctacac cgtgtatttc gagaatgacc gcgtcatcca cagcgtcctg
     2401 aacgctgctc cctgaattca cttttccccg gggcggtaat ccgctatttt accgcctcgt
     2461 tccactacct tcccgctgca atcatgactg aacgtttttc cctgctgccc gaatgggctg
     2521 aacaggaagc tgtgctcatg gcatggcccc acgaatccac cgactggcaa ccctggctgg
     2581 cggcgatcca gcacgattac gtggaactgg ccgtggccat tgcgcgggaa gtgccaccgg
     2641 tcatcctgtg tcaggacgag gcgcaccgcg atctgatcct gcgtcagctg gcaggtcgct
     2701 gcgaacaggc acccgtgctg gtaatcgctc cctacaacga tacctggtgc cgggactatg
     2761 gccctataac gctgggcccc attaccctgg gcccggcggg caataccggc cagcgccgcc
     2821 tgctggactt ctgcttcaac ggctggggcg acaaatacga cgcggaactg gacaacggca
     2881 tcaaccgctc catcgcccag gtgtggaagc cggaactgca gaccatcgat ttcgaactgg
     2941 aaggcggcag cattgaaacc gacggcgccg gcaccctgct caccaccacc cattgtctgc
     3001 tggacagcaa ccgcaacaac gatttcaaca aggaacagat cgaagccttc gtgctggaaa
     3061 aactgggcct gcaacgctgc ctgtggctga gccgcggcgc cctgttcggt gacgacaccg
     3121 acagccatat cgacaacctg gcccgcttct gcagcgccga caccatcgcc ttcgccacct
     3181 gttccgacgt gaatgatccg caccacgaac acttgcaggc gatggaagca gagctgcaac
     3241 agttccgtca gcccaacggc agaccctacg tgctgaaggc gatcgaaatc ccgcagccgc
     3301 agttcgacga gaacggcaag cgcctgcccg gcagctatat caattttttg atcgtcaaca
     3361 acagtgtcat cgtgccggtg tttgattgcc cgcaggacca ggccgcgctg cagaccctgc
     3421 agcagtgctt ccccggcaaa cggctgctgc cggtcccggg caaaaacctg atcaagcagt
     3481 atggcggccc gcactgtgcc accatgcagc tgcccaaagg caccactctc atcaaccgtt
     3541 aacccgtttc atccgttcaa aggttcgagc aagatgagca acaagaccct gcgcgttggc
     3601 gtgatccagc aaccggcctg gcctgacaag gccaagagcc ttgccgaaac cgaacgtctg
     3661 ctgaaacagc tggtggacgc gggcaaaccg gaactggtgc tgctgcagga actgcactgc
     3721 acgcattatt tctgtcaggt ggaagacacc gcgctgttcg atctggcgga accgctggac
     3781 ggcccgagtg tgcagaccct gtcgcgctgg gcgaagcagt acaacaccgt gctggtggga
     3841 tcgatctttg aaaaacgggc gcccggcgtg ttccacaaca ccgccgccgt gtttgaaaag
     3901 gacggttcgc tggcaggcac ctaccgcaag atgcacatcc ccgacgatcc gggtttctac
     3961 gaaaaattct atttcacccc cggcgatgcg gtgaaactga acggcgacag cggcttcacc
     4021 ccgattccca ccagtgtggg caacctgggc gtgctggtgt gctgggacca gtggtacccg
     4081 gaaggcgcgc gcctgatggc catggccggc gccgacctcc tgctctaccc cactgccatc
     4141 ggctgggacc cgcgcgacac cgacgacgcg caggcaaaac agaaaaatgc ctggcaactg
     4201 atccagcgct cgcatgccgt ggccaaccac ctgccggtga tcgtggcgaa ccgcgtcggc
     4261 catgaagacg atccgtctgg ccagagcgac ggcatcctgt tctggggcgg cagtttcatc
     4321 agcggccagc agggcgacat cctctgcgaa ggcgacgcgc acaccgcaca atacctgatt
     4381 gccgaggtgg aactgggtcg cactgaatac ctgcgccgca tgtggccgta tttccgcgac
     4441 cgccgcatcg atgcctacgg cggcctgacc cagcgctttc tcaaaccctg attgttctgc
     4501 gagacagatt ttcccgcgaa cctgattttt ccgcgaagga gttaacccgc atgctgatca
     4561 tcggtcaccg tggcgcgcgc cgggaagcgc cggaaaacac cctcgccggc tttcggtatc
     4621 tgcgctccct cggtgtgcac cacgtggaac tggacgtacg cctgtcgaaa gaccagcagc
     4681 tggtggtgat ccacgacgac accctcaacc gcaccaccgc ccacaacggc aatgtccgcg
     4741 atttcacggc ggcggaactg gcccaggtga atgccgcagc gaaatatgcc ggcggctggc
     4801 cggaggccac accggtgccg ctgctgcgcg acgtactgaa agagtgggac gatctggaaa
     4861 gcatccagct ggaagtcaaa accactgatg agaacgacct gcgcaccatc gcccacctga
     4921 ttcgtgacct ggtggatgaa ctggacctga tcgataccgc catcgtcacg tcgatggaca
     4981 cgcacctgct gggctacatg caccagaccg cacccgaaat ccggcgcggc tacgtggccg
     5041 agcgcttcac ccgcgagccg ctggccatgt gccagctgca ccactgcacg catctgatca
     5101 ccaactatca ccgcgtcacg ccgggtctgg tacagaccgc acacactatt ggcctggaag
     5161 tatcggtgtg gacggtgaac gatctcaata tcgcccaccg catccagcac tgcggcgccg
     5221 acagcatcat caccgacgtg ccatcgctga tgctgaacaa cctgcgccat gacatcgaat
     5281 actaaacgcc tgctcaccac actgctggca ggcgccgcca cactgctgca aggctgcgac
     5341 gccagcttct actggcaggc cgcccggggc cagttcgacc tgctgcaacg gcgccaggac
     5401 atcccagcgt tgctgcagtc cgacagcacc gatgcaaacc tgaaacgcca actggaatac
     5461 gtgctgcagg tgcgtacctt tgcggcggag cagctgcatc tcaccgtcaa cgacaattac
     5521 ctgacctacg tcgacctgca acggcccttc gtggtgtgga acgtgttcgc cacgccggaa
     5581 ctgtcgctgg aaaaccggcg ctggtgtttt ccggtagccg gttgcgtgcc ctatcgcggc
     5641 tattttgccg aaaccgatgc tcaggcctac gccggcaccc tggcacagca aggcttcgac
     5701 acctacgtgg gtggcgtggc cgcctacagc accctgggct ggttcgacga tgccgtgctg
     5761 aacaccttcg tcagccgcga tcagctgtca ctggcggcgc tgctgatcca cgaactggcg
     5821 caccagaccc tgtacgtacc cgacgattcc actttcaacg aaagcttcgc caccgcggtg
     5881 gaaaatctgg ggctggaacg gtggatcgaa caggccggat tgcaggatca gctgccagcc
     5941 taccgtgagg caaaacaacg ccagcagcaa tttctggatc tggtgctgac acaccgggac
     6001 gaacgggaaa agctgtttgc ctcatccctt accgatgcgg aaaaacgcca gcgcaaacag
     6061 gaactgcagg ccgcactgca actgtcctac ctgcagctga agcagtcctg gaacggatat
     6121 gacggctacg accgctggtt ctcgcgcccg gtcaacaacg cccagctttc gacggtcgct
     6181 acctaccacg cactggagcc cggatttcgt gccctgtttg aacagagcgg gcaccatttt
     6241 cccgcattct tccagcgctg ccgcgaaatt gcccggttac ccaaggacca gcgcgacgcc
     6301 ctgctgcaac gccgctgaag cacaccctgc cgcaactttc tgccagccgg cgcagtcacc
     6361 ccgatcggca cctggccacc gccaccgcct gcctccggag acttcgtcta tactgtttct
     6421 aaacgtttga attccggatc gaatcccttg tatgagcatg cccgtcagcg acaagtgcga
     6481 accttttatc cacctggaca agttgattcc agtgctgtcg gatctgtata cggcgatcaa
     6541 atcacgcaat gaaaaatgcc aggagatgtt gcagggcctg atcagccgga tcaatcgcat
     6601 tgccgacacc catccggacg ccaccatcgc agccatccat ctgtcgaaac agcagtcacc
     6661 gctgaaacag cccatttatg gcgcgattct ggcgcatctg ttcagtcagc agcatggtct
     6721 ggatgacaag aaggaacagg cgctggccca ggccgtactc acctgcaatc tcacctttta
     6781 cgaattccag gtactgctaa acagcatgga cggcaaactc accgacgcgc agcgcgccaa
     6841 gttgcagaag catccgctgc aggcagccca atatatggaa gcggcaggat tcatcgatcc
     6901 ccaactgacc aaggccatcc gccagcacca cgaacggccg gacggaagtg gttatccgaa
     6961 tcatctgaaa gggccggaca tttccgatct ggcactggtg gtgtccatgt gcgaggtgta
     7021 cacggcgcgc atcgacaacc gcgcctaccg caagccggtg ctggcacggg aagcactgtc
     7081 gcacctgtac aaggaagatg atccgcgtct gaaaggtctg ttgctgagtt ttgccaaggc
     7141 tgtcggcgtg tatccgccgg gtacctgggt gaagctggcg aacaatgaga ccgccatggt
     7201 gacgcggcgg cagaagaaca gcccggtgcc catcgtgaag gcgctgttcg accgcaacga
     7261 tattccctac atggggcccc tcacccgcga ctgcaacgat cccgccctca aggtcgtcgg
     7321 ggccactttc
//
LOCUS       DASUEG010000873         6895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1659416, whole genome shotgun sequence.
ACCESSION   DASUEG010000873 DASUEG010000000
VERSION     DASUEG010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659416"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VFX11_16465"
     CDS             complement(<1..1378)
                     /locus_tag="VFX11_16465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4940269.1"
                     /translation="MSQVQDVPSLQRALRESEQQFRLLVESVRDYAIFMLDAEGRIAS
                     WNAGSQRIKGYTAEEVLGKHFSIFYTPEDIAKGMPQKELDCAMREGRCEVQGWRLGRD
                     SARFLAHVVITPIVDESGRLRGFAKVTRDISLHHAAEQRFRQVVESSPSAMVMINMQG
                     VIEMVNTQAERIFGHSRAELLGQQVELLLPMRLSQHHPQLREAFFSAPQSRPMGAGRD
                     LFARRKDGSEFPVEIGLNPIETDEGTKVLSAIVDISDRKQKEQKIQAALEEKNILLGE
                     IHHRVKNNLQIVHSLLDLQSSRVSDPVALEMLRDSQNRIRSMALIHQSLYLSNDFAQV
                     NLGNVLEALIPALLESYSVDHSLIRYEFTVDDVLLSINQAIPCGLIINELISNALKHA
                     FSDGRSGTIHVELAQKEQDYVLLSVSDDGAGIPPDVDLKKTDTLGLQLVHLLADQLGA
                     TLTINRAEP"
     gene            1654..2196
                     /locus_tag="VFX11_16470"
     CDS             1654..2196
                     /locus_tag="VFX11_16470"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX4940270.1"
                     /translation="MKKLAIGKAFTLMESGPVVLVTTHDGKRDNVMTISWTMVLDFTA
                     RFAITTGSWNHSYTALRKTRECVIAIPTVDLLDTVVGIGTCSGADTDKFSRFGLTPVK
                     GKKVKAPLIRECLANIECKVVDIINKHNIIVLEGVAAYVDVSRKEKRTLHAIGDGTFI
                     VDGRKLSRKKMMADKIPPGI"
     gene            complement(2253..3092)
                     /locus_tag="VFX11_16475"
     CDS             complement(2253..3092)
                     /locus_tag="VFX11_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940271.1"
                     /translation="MKIRGSTITYNDTTKKITTRVDARIDGILTGEYTNFWAVMNDQL
                     SITERYVGYGQAPWFNDYVSEAWCSGTSKVDYDGSVDTFVANIVLNVADPIITAFNNG
                     QPISINNVLNKSARMLQGPGCHLAKPFTARQFLVPNTDMKLVLDFPVLQVQGGVTVLG
                     RYALAWRNMSVSINGLAGPGILYADPNEQPEGQFYATPRDLDSFLTYTWEAPGAIIAQ
                     PNAQSTKIRWPITVTSGQQVSKTVKVTVRDGSGHVVSKQESVLIRSSDTIPETDCVKY
                     ICD"
     gene            complement(3144..3767)
                     /locus_tag="VFX11_16480"
     CDS             complement(3144..3767)
                     /locus_tag="VFX11_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940272.1"
                     /translation="MTAMNQLGPQRDQRFLLVATLLLGFATSLQPGQAPHAAICQNCE
                     ATPVPDVPTYIPPPPPMVALQFDQCIFSDYSVFSMVMDKPAFTNVVAGTANGVFKQMR
                     NALGVYDRYQTEANRTDREPFLHPALWPNLDIRIEDCVQQDAGGYGTNVAVWFEQPGY
                     YGSSDTTESRNWWAKNMNYREVNNQSGQVGIHIDDRALQSMLSIAKA"
     gene            complement(3976..5016)
                     /gene="arsB"
                     /locus_tag="VFX11_16485"
     CDS             complement(3976..5016)
                     /gene="arsB"
                     /locus_tag="VFX11_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019933766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX4940273.1"
                     /translation="MGMFERYLSLWVAMCIVAGVTLGNLMPDLFVLIASLEWAHVNLV
                     VAVLIWIMIYPMMVQIDFSAVKNVGKKPQGLMLTLVINWLIKPFTMAALGWLFFKAVF
                     TPWVDPALATEYIAGMILLGVAPCTAMVFVWSQLTKGDPNYTLVQVSVNDLVMVVAFA
                     PIAAFLLGVSDIEIPWETLLISVVLYVVLPLIAGTLTRHRLESRGDGVRLKSFLASVK
                     PWSVMGLLLTVVLLFGFQAEVILDQPYAIVLIAIPLLIQSYGIFFITYAAARKMKLPY
                     DVAAPASLIGTSNFFELAVAVAISLFGLHSGAALATVVGVLVEVPVMLSLVAFANRTQ
                     YWFQPGASRIPQ"
     gene            complement(5016..5489)
                     /locus_tag="VFX11_16490"
     CDS             complement(5016..5489)
                     /locus_tag="VFX11_16490"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX4940274.1"
                     /translation="MKILFLCTHNACRSILAEAIARKMGRGRWQVASAGSQPSGRVHP
                     LTLHYLAEAGYSTDDLHSQGWDSLEEFQPDVLITVCDQAAGESCPVWLGRAMKVHWGL
                     ADPSRNAPGSPEADAAFRYTIRLLEGRFEALLKHDLDQLAPAQLQQIFIETGASR"
     gene            complement(5535..5879)
                     /locus_tag="VFX11_16495"
     CDS             complement(5535..5879)
                     /locus_tag="VFX11_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011070872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4940275.1"
                     /translation="MNPVQFYKCLADDTRLRCLLLIAREQELCVCELTEALQEIQPKI
                     SRHLAQLRENGLLLDRRQGQWVFYRINPAMPDWAKAVLSTTLDSNPAFLAKNLKQLCR
                     MGDRPTRTQACC"
     gene            complement(5984..>6895)
                     /locus_tag="VFX11_16500"
     CDS             complement(5984..>6895)
                     /locus_tag="VFX11_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEX4940276.1"
                     /translation="ALRAKGWTAFCPLDLIDRQSPQEFARWGKTVGELIGTDLTLEHN
                     PERPHTTPLQLAERVEYAADTDTPTTVFRFIAPPPARKAGILRFLRRHRGLPYFEAGD
                     LVGILPPGSHVPRFYSLASSSKEGLLEICVRKHTEGLCSGYLHNLKTGETIDAFIRPN
                     PEFRPASGKTPVILIGAGTGIGPLTGFIKHNTPHHPMYLYWGGRNPQSDFLYQSELNG
                     YLEDKRLTELNTAFSRTDDGAYVQDRISADAAELRQLIQKGAQILVCGGREMAKSVRD
                     AIDAIIAPLDTSVQALRAEGRYREDVY"
BASE COUNT         1528 a         2008 c         1908 g         1451 t
ORIGIN      
        1 tgggttcggc ccggttgatc gtaagagtcg cacccagctg atcggccagc agatgcacca
       61 gctgcaatcc gagggtgtcg gtttttttca ggtccacatc cggcggaatt cccgcaccat
      121 cgtcgctcac cgacagcaac acgtagtcct gctccttctg cgccagctcc acatgaatgg
      181 taccgctgcg tccgtcggaa aatgcatgct tgagtgcatt ggaaatcagt tcgttgatga
      241 tcaggccaca gggaatcgcc tgattgatcg agagcagcac atcgtcgacc gtaaattcgt
      301 agcggatgag gctatggtcg acactgtagg attccagcag cgcgggaatc agcgcttcca
      361 gcacattgcc cagattgacc tgggcaaaat cattggacag gtacagtgac tggtgaatga
      421 gtgccatcga acgaatccgg ttctggctgt cccgcagcat ttccagggcc accggatcgg
      481 aaacacgcga ggattgcagg tccagcaggc tgtgtacgat ctgcagattg ttctttacgc
      541 ggtggtgaat ttcacccaac aggatatttt tctcttcgag cgcagcctgg attttctgtt
      601 ccttctgctt gcggtcggag atgtccacaa tagccgaaag caccttggtg ccctcatcgg
      661 tttcaatcgg attgagtccg atttccaccg gaaattcgct gccatccttg cgccgggcaa
      721 acagatcgcg gcccgccccc atcggccgtg attgcggagc agagaaaaag gcttcccgca
      781 gctggggatg gtgctggctc aaacgcatcg gcagcagcag ttcaacctgc tgacccagca
      841 attccgcacg ggaatgtccg aaaattcgct cggcctgggt gttcaccatc tcgatgacac
      901 cctgcatgtt gatcatcacc atcgcgctgg gtgatgattc caccacctgg cgaaagcgtt
      961 gctcggcagc atgatgcagg gaaatatcac gggtgacctt ggcaaagcca cgcagccttc
     1021 cgctctcatc gacaatcggg gtaatgacaa cgtgggccag aaagcgggcg gaatcccgac
     1081 ccaggcgcca accctgcacc tcgcaacgcc cttcgcgcat tgcacagtcc agttccttct
     1141 gcggcatgcc cttggcaatg tcttccggtg tgtaaaaaat cgagaagtgt tttcccagca
     1201 cttcttcggc ggtgtatccc ttgatgcgct gggaacccgc gttccagctg gcgatacggc
     1261 cttccgcgtc gagcatgaag atcgcgtaat cacgtacgct ttccaccagc aaccggaact
     1321 gctgttcact ttcgcgcaac gcccgttgca gcgaaggtac atcctgtact tggctcatgc
     1381 tcatcccttg gtccgattga tccaatatct tataaacata gtcgattatg acctgccagc
     1441 gcgggccggg gttctggtta gttcgcatcc cgacaaaacc agaaaaaaat cacaaaaata
     1501 cacgcactgg aagtatgcgt actcgcaata catcttcatt gagtaaggtt atagtcaggc
     1561 gaacacaggg tttacgccgt ccatggcacc atattcgtgc accggtggca cgcacattcg
     1621 aatacggaat tctgatgtcg aggaacgcag caaatgaaaa aactggctat tggcaaagcc
     1681 tttacattga tggaatccgg gcctgttgta ctggtgacca cccacgatgg aaaacgtgac
     1741 aacgtgatga caatctcatg gaccatggtg ctggatttca cggcaaggtt cgccatcact
     1801 acaggcagct ggaatcattc ctacaccgcg ctgcgcaaaa ccagggagtg tgtcatcgcc
     1861 attcccacgg ttgacctgct ggatacggtg gttggcattg gcacctgttc gggcgctgac
     1921 acggacaagt tttcccgctt cggtctgacg ccggtaaaag gcaaaaaggt gaaggcaccc
     1981 ctgatccgcg aatgcctggc caacatcgaa tgcaaggtcg tcgacatcat caacaaacac
     2041 aacatcatcg tactggaagg cgtggcagcc tacgttgatg tttcccgcaa ggaaaaaagg
     2101 acactgcacg ccattggcga tggcacgttc atcgtggatg gccgcaaact gagccgaaaa
     2161 aaaatgatgg cagacaaaat cccgcccggc atttagtgat gccgataccg ggcgggatct
     2221 ggcgttgaac atttatgcaa gcacgcggac cgtcaatcgc agatatattt cacgcaatct
     2281 gtttcgggaa tcgtatccga cgaccggatc aaaactgatt cctgcttgct gaccacatgg
     2341 ccgctgccgt cgcgcacggt caccttcacc gtcttcgaca cctgctgccc actggttact
     2401 gtgatcggcc agcggatctt ggtggattgc gcgttgggct gggcgatgat cgctcccggt
     2461 gcttcccagg tgtacgtcag gaagctgtcc agatcgcgtg gcgtggcata aaactgccct
     2521 tccggttgtt cgttgggatc ggcatacaga atgcccgggc cggccaggcc attgatgctg
     2581 accgacatgt tgcgccacgc cagagcataa cgtcccagca cggtgacgcc accctgcacc
     2641 tgcagcaccg gaaaatccag taccagtttc atgtcggtgt tgggcaccag aaactgccgg
     2701 gcagtgaacg gcttcgccag atggcagccc ggcccctgca gcatgcgcgc gctcttgttc
     2761 aacacgttat tgatactgat cggctgacca ttattgaacg cagtgataat cggatcggcc
     2821 acgttcagca caatattggc gacaaaggta tcgacgctgc cgtcatagtc cactttcgat
     2881 gtgccgctgc accaggcttc gctgacgtaa tcgttgaacc agggcgcctg gccatatccc
     2941 acgtaacgtt ccgtgatgct gagttgatca ttcatcactg cccagaagtt ggtgtactca
     3001 ccggtgagga tgccatcgat gcgagcgtcc acccgcgttg tgattttctt ggtggtgtcg
     3061 ttataggtaa tggtggaccc gcgaatcttc actttgtttt ccagcgcaga gcccttgacc
     3121 tgcttgtcga tctgccgctg gccctaggcc ttggcgatgc tcagcatgct ctgcagtgca
     3181 cggtcgtcga tatgaatgcc cacctgcccg ctttgattgt tcacctcgcg gtagttcatg
     3241 tttttcgccc accagttacg cgattcggtg gtatcggacg aaccgtaata acccggctgc
     3301 tcaaaccaca cggcaacatt ggtaccatag ccgccggcgt cctgctgcac gcaatcctcg
     3361 atgcggatat ccaggttcgg ccacaacgcc ggatgcagga acggctcgcg atccgtacgg
     3421 ttggcttcgg tctggtagcg atcataaacg cccagtgcat tgcgcatctg tttgaacaca
     3481 ccgttcgcgg tgccggcgac cacattggtg aatgcgggct tgtccatcac catgctgaac
     3541 acgctgtaat cgctgaaaat acactggtcg aattgcaggg ccaccattgg cggcggcggg
     3601 ggaatataag tgggaacatc cggtactggc gttgcttcgc aattctggca gatggccgcg
     3661 tgcggcgcct gaccgggctg caggctggtg gcaaatccca gcagcagcgt tgcaacaaga
     3721 agaaagcgct ggtcgcgctg cggccccaat tgattcatgg cagtcattgt cttaaccctt
     3781 gatccgtcga tgtgaaaaat ggctcgacgg gcgacttccc tgactgctac tgcttttcct
     3841 tcagccagtg acacccgatg cggggcgcat gataaagatg ccgccccgga cggtcatgtc
     3901 actttttcgc aaaaacggct gcagtttagt cacgtcctgc gtatgacttt cgttcggtgg
     3961 tgatgctgaa gctgattatt gcggtatacg cgatgcgcca ggctggaacc agtattgcgt
     4021 ccggtttgca aaggcaacca gcgacagcat caccggcact tcaaccagca cgccgaccac
     4081 ggtggccagg gccgcaccgg agtgcaggcc aaacagtgag atggcaaccg caaccgccag
     4141 ctcaaagaaa ttcgatgtgc caatcaggct ggccggcgca gcaacgtcat agggcagctt
     4201 catcttgcgc gcggcggcgt aagtgatgaa gaaaatgcca taactctgaa tcagcaacgg
     4261 aatggcaatc agcacgatcg catagggttg atccagaatt acctcagcct gaaagccgaa
     4321 cagcagcaca accgtcagca acaatcccat gacagaccag ggttttacac tggccagaaa
     4381 cgatttcagc ctgacaccat ccccccggct ttccagacga tggcgcgtta gggtgccggc
     4441 gatcagcggc agtacgacat agagcaccac ggaaatcagc aaggtctccc agggtatttc
     4501 aatatcgctg acacccagca gaaacgcggc aatcggtgcg aacgccacca ccatgaccag
     4561 atcattgacc gatacctgaa ccagcgtgta attgggatcg cccttggtga gctggctcca
     4621 cacgaaaacc atcgcagtac agggcgccac acccagcagg atcatgccgg cgatatattc
     4681 cgtcgccagc gcgggatcga cccagggcgt gaacaccgct ttgaaaaaca gccagcccag
     4741 cgcagccatg gtgaatggct tgatcaacca attgatcacc agcgtcagca tcaggccttg
     4801 tggttttttg ccgacgtttt tcaccgcaga gaaatcgatc tgcaccatca tcggatagat
     4861 catgatccag atcagaacag caaccaccag attcacatgc gcccattcaa gactagcaat
     4921 caggacgaac agatcgggca tcagatttcc cagcgtcacg ccggccacaa tgcacatcgc
     4981 tacccacaac gacagatacc gctcgaacat gcccatcagc gtgatgctcc tgtctctata
     5041 aaaatctgct gcagctgcgc cggtgccagt tgatccagat cgtgcttcaa caaggcttca
     5101 aagcggcctt ccagcaggcg gatcgtgtag cggaatgccg catcggcttc tggcgatcct
     5161 ggcgcattgc gggaaggatc ggccagcccc caatgcactt tcatcgcgcg ccccagccac
     5221 accgggcagg attctccggc agcctgatcg cataccgtga tcagcacgtc cggctggaat
     5281 tcttccagtg aatcccagcc ctggctgtgc aggtcgtccg tgctgtagcc cgcctccgcc
     5341 agatagtgca gggtcaacgg atgcacgcgg ccggagggtt ggctgccggc actggccacc
     5401 tgccagcggc cgcgccccat tttacgggcg atagcctccg ccagaatgct gcggcaggca
     5461 ttgtgtgtgc acaaaaacag tattttcata gcgtcatcca gagaccaaca tcaggcgctt
     5521 cagcaacagg cttttcagca acaggcctgg gtacgtgtgg gcctgtcacc catacggcaa
     5581 agctgtttaa gatttttcgc cagaaaggcg ggattgctgt ccaacgtggt ggagaggacg
     5641 gccttggccc agtccggcat tgccggattg attcgataaa acacccactg gccctggcgg
     5701 cggtccagca gcaaaccatt ttcccgcagc tgcgccagat gacgggagat tttgggctgg
     5761 atctcctgca gcgcttcagt cagctcgcac acgcacagct cctgttcccg ggcaatcagc
     5821 agcaggcagc gcagccgcgt gtcatcggcc agacatttgt agaactggac tggattcatg
     5881 ggtgtactcg caggagctta atccgtgtcg gtcgggatta tatacgaaat ttcatatata
     5941 ttaaaagtgc ccacggcaat gcaggtctga tcccgggatg gaatcagtaa acatcctccc
     6001 gatatcgccc ttcggccctc agcgcctgca cgctggtgtc gagcggtgca atgatcgcgt
     6061 cgatggcgtc cctgacgctt tttgccatct cccggccacc acacaccaga atctgcgcac
     6121 ctttctggat cagctggcgc agttccgcgg catccgcact gattctgtcc tgcacgtagg
     6181 cgccatcatc cgtgcgggaa aacgccgtgt tcagctcggt caaacgcttg tcttcgagat
     6241 agccgttcag ctctgactga tacagaaaat cagattgcgg attgcgtccg ccccagtaca
     6301 ggtacatggg atgatgcggt gtgttgtgct tgatgaatcc ggtcagtggc ccgatgcccg
     6361 tgccggcgcc gatcagaata acgggcgtct tgccggaggc cggccggaat tccggattgg
     6421 gccggatgaa ggcgtcgatg gtttcaccgg ttttcagatt gtgcaggtag ccggaacaca
     6481 ggccttcggt gtgcttgcgc acgcagatct ccagcagccc ttccttcgat gaagacgcca
     6541 gcgaataaaa ccggggaacg tgactgcccg gcggcaggat gccgacgaga tcgccagcct
     6601 caaaataagg aagaccacgg tgccggcgca gaaagcgcag gatgcccgcc ttgcgcgctg
     6661 gcggtggggc gatgaaacgg aatacggtgg tgggcgtatc cgtatcagcg gcgtattcca
     6721 cgcgctccgc cagttgcagc ggcgtggtgt gcggacgttc cggattgtgc tccagcgtca
     6781 gatccgtgcc gatcagctcg cccacggttt taccccagcg ggcaaattcc tgcggcgatt
     6841 gccggtcaat caggtcgagc gggcagaagg ccgtccagcc tttggcgcgc agtgc
//
LOCUS       DASUEG010000874         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1580153, whole genome shotgun sequence.
ACCESSION   DASUEG010000874 DASUEG010000000
VERSION     DASUEG010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580153"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2128)
                     /gene="hrpA"
                     /locus_tag="VFX11_16505"
                     /pseudo
     CDS             complement(1..2128)
                     /gene="hrpA"
                     /locus_tag="VFX11_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032333.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
BASE COUNT          407 a          674 c          723 g          484 t
ORIGIN      
        1 tctcggccgc cacgatccat ttcggcttgc gctggtggca ggcggaaccg ggaaaaatct
       61 gcacggtgcg attgcgggca gccgtgtagc ctttcagttc atccttcatc gccacgtggc
      121 tgagcaggcc ggacaaaatc gccttgtgga tttcctcgta gcgggccggc tcggtgtttt
      181 cctgccagcc cagttcgtgg cacacctgct tcagctggcg atacacatcc tgccactcgc
      241 gcatgcgcag atagctgaga aaacgttcca gcagccagcg ccggtagcgg ttgttgctca
      301 gttcttcttt ctgcttttca ctttcctgcc agatgttcac ataggtgaga aaatccgact
      361 cctcgccctg gaacttgcgc agggattcgt ccgccgcacc ctgccggtca tgggggcgtt
      421 gccagggttc ctgcacgctc atgccgctgg caatgatcag catctcccgc aggcagtgat
      481 attgtttggc ggcgatgatc atgcgaccga tgcgggggtc caccggcagt ttcaccagat
      541 cgaaacccaa cggggaaatc tggcgtttgc gatccaccgc gttcagttct tccagcaggc
      601 ggtagccatc gttcaccagg cgggaatcgg gcggatcaat gaacgggaag cgcgacactt
      661 cgcccagttt caagccctgc atctgcagga tgacagacgc caggttggtg cgcaggattt
      721 ccggctcggt aaattccggg cggttgagaa aatcggtttc gtcatagagc cgaatgcaga
      781 tgccttccgc aacacgtccg caacggccca tgcgctggtt ggcgctggcc cgcgacaccg
      841 gctcgatcgg caggcgctgc actttcgtgc ggtaactgta acggctgatg cgcgccagac
      901 cggtgtcgat cacgtatccg atattcggca cggtgatgga ggtttccgcc acgttggtgg
      961 acagcacgat gcggcggccg gtgtgggtct ggaaaatgcg gttctgttcg ctgttgctga
     1021 ggcgggaata cagcggcagg atttcggtgt gcttcggccc gttcttgcgc aggcgatccg
     1081 ccacctgacg gatttcccgc tcgccggaca gaaataccag aatgtcgccg gggcgacgcg
     1141 gattgggatg cgcacgctcc agctgcacaa tttcttccac ggcgcgcagc acaccggttt
     1201 ccagcaggcc ttcgtcgtcc ggcacctgtt cgtcatcgct gctgtcgtcg gtttgcagcg
     1261 gacggtacag cacgtccacc gggaaagtgc ggcccgacac ttcaatcacc ggcgcatcgt
     1321 tgaaatggcg ctagaaacgc cagtggtcga tggtggcgga cgtgatgatg actttcagat
     1381 ccggacgctt cggcagaatc tgacggagat aacccagcag gaaatcgata ttgaggctgc
     1441 gttcgtgcgc ctcgtcgatg atgatggcgt cgtagccgtt gaggaagcgg tcgttgggaa
     1501 tttcggccag caggatgccg tcggtcatca gtttgatcag gctgtgctcg cccagctgat
     1561 cctggaaccg caccttgaaa cccgccacct gccccagcgg cgatttcagt tcgtcggcga
     1621 tgcgctgggc cacgctgcgg gccgccagcc ggcgcggctg cgtgtggccg ataaagccgg
     1681 tcacgcccag gccggcctcg atgcacatct tggggatctg ggtggtttta ccggaaccgg
     1741 tctcgccggc gatgatcacc acctgatgtt cgcggatagc cgagatgatg tcgtcgcggc
     1801 gctgggacac cggcagatcc gggtattcca gaacaggaat gctgttgcgc cgcgccgtaa
     1861 ccgcctgcag ggatcgttcg atgctggcct gcagtttttg caggtcgcgc tgcagtggtg
     1921 ccgtatcaga ctgcggcgct gacttctgaa gctccggctt ctgctgtcct ttctgtcttt
     1981 ccttctgctg caactggtcc agactgcggg ccagccggta gcggtcgcgg gtcaggcaca
     2041 aggggatctg ccggcgcagg gcagccagat cgggcttggt ttcggggttt ggttccggct
     2101 tttccacagg cttccagcgg cacaacattg gccgtcttca attcaatcag ggggcgcatt
     2161 gtacaaaaaa gccggggccg gaaaaaacaa agcctgcgcg gggcaggctt ggctcgagtg
     2221 ccagcatgaa cgaagcggca ggtatttgaa tccggacacg gactgggcgt ccccccgtga
     2281 acccatcc
//
LOCUS       DASUEG010000875         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1933663, whole genome shotgun sequence.
ACCESSION   DASUEG010000875 DASUEG010000000
VERSION     DASUEG010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933663"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..793)
                     /gene="plsY"
                     /locus_tag="VFX11_16510"
     CDS             complement(167..793)
                     /gene="plsY"
                     /locus_tag="VFX11_16510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX4940277.1"
                     /translation="MLLYSLLAVVLWTFSYLLGSVSTAILVCRLFGYPDPRSTGSHNP
                     GATNVLRVGGKTAAIVTLIGDVAKGVVPVLIARQLGLPDSIAAFCGFCAFLGHLYPVY
                     FDFQGGKGVATAMGAVAALHWPSALAIGLVWLVVAATTRISSLAAIISFFTAPMLVLL
                     IRADLFYPILVLSLMLLFRHRVNIMQLIRGEELAFGSKESGDGRSPQE"
     gene            880..1236
                     /gene="folB"
                     /locus_tag="VFX11_16515"
     CDS             880..1236
                     /gene="folB"
                     /locus_tag="VFX11_16515"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX4940278.1"
                     /translation="MDIVFIRELKIQTVIGVFEWERAIRQTVVLDLEMAADVARAAAT
                     DRLEDALDYKAVTNRVVQFVEDSRFQLVETLAESIATILRDEFGVPWLRLRVSKPGAI
                     HGAKDVGVLIERGGAG"
     gene            1233..1772
                     /gene="folK"
                     /locus_tag="VFX11_16520"
     CDS             1233..1772
                     /gene="folK"
                     /locus_tag="VFX11_16520"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4940279.1"
                     /translation="MSTTVFLGLGSNDQPRRNIRLALDLLQECFGALQVSPVFANAPL
                     ATAAVAPDAELYFNLVVSAVCNVPAGELKQQLRRIEERCGRVHASGDCLQKNPVCSLD
                     IDLLLYGDAVGDVDGVVLPHPDVLRRAYVLYPLSLLVPSGRHPQLQQTFAELWRGFGK
                     SRWELKPVAPFLPDYSLQQ"
     gene            complement(1832..2578)
                     /locus_tag="VFX11_16525"
     CDS             complement(1832..2578)
                     /locus_tag="VFX11_16525"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX4940280.1"
                     /translation="MTTDSSRKVALITGSARRIGAHTADYFHQRDYDIVLHYRTSGDG
                     AKNLRDRLNSVRPHSCIALHADLSDPAAWEQLAEQVRSWKHRLDLLVNNASSYYPTEF
                     GNTTLSQWNDLFASNAMAPFFLTQHLLPLLKQSRGCVVNIVDINADKPLPDFTPYCMA
                     KAALKAMTLSLARALAPDVRVNGVSPGTILWPADGNMPDAVQQAILTNTPMQTIGSPD
                     DIAKAIYFLAEQAPFVTGQIIAVDGGYSLS"
     gene            complement(2589..>3538)
                     /locus_tag="VFX11_16530"
     CDS             complement(2589..>3538)
                     /locus_tag="VFX11_16530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4940281.1"
                     /translation="TLVDPFHGQQDLQNRLLRHVSPAFVEDPLRVLRVARFLARFHDR
                     GFQIADETLALMRDIAASGELQTLTPERVWTETEKALLEPSPWRYFEALRDCGALAVV
                     FPELDKLFGVPQPPKHHPEIDTGLHVLLTLQTALKLMQTHPLEKADRVAVLFSLICHD
                     LGKGMTPEFKWPSHPGHEPISAHLAEKLCLRLRAPNPVKRLGLLVAEFHTHCHRAFEL
                     KPATVMRLLEALDALRRPETLPLFLLACEADARGRTGLEDRDYPQADYIRDCAVAARR
                     VEAKPLVELGFKGETLGIELRKRRIVAIRDVKNCYVKEQ"
BASE COUNT          689 a          998 c         1156 g          695 t
ORIGIN      
        1 ccagggcgga tctggcagtg cgggcgcggg cgcgctggcc gttgtcggag ctgaaggggc
       61 gggatcaggc ggatgcatcc cgggttggtc aggattccag accggcttga actggcgggg
      121 tttcagtgga ttgttctgcc agcatccggc gtggaaatgc ggctggtcat tcctgcggtg
      181 aacgtccgtc accggattcc ttgctgccga aggccagttc ctcgccccgg atcagctgca
      241 tgatgttcac ccgatggcgg aacagcagca tcagtgacag caccaggatc ggatagaaca
      301 ggtcggcccg gatcagcaat accagcatgg gcgcggtgaa aaagctgatg atggccgcga
      361 gtgaactgat gcgggtggtg gcagcaacca ccagccagac caatccgatg gccagtgccg
      421 aaggccagtg caacgccgcc actgcaccca tcgccgtggc cacgcccttg ccaccctgga
      481 agtcgaaata caccggataa agatggccca gaaaggcgca gaacccgcag aaggcggcaa
      541 tgctgtcggg cagcccgagc tggcgggcaa tcaacaccgg caccacgcct ttggcaacat
      601 caccaatcaa tgtgacaatg gccgccgtct tcccacccac ccgcagtaca ttggtggcgc
      661 ccgggttgtg ggagccggtg gagcggggat cgggatagcc gaacaggcga cacaccagga
      721 ttgcggtgga aaccgatccc agcagatagg aaaatgtcca gagcacgacg gccagcaggc
      781 tgtacagaag caactccacg cgggtggtta aacaatgatg aaagaattgt ttcacttcgc
      841 tgccggtagc tcaagcgtaa tcagaaacag gcgggccaga tggatattgt tttcatacgc
      901 gaactgaaaa tccagaccgt gattggtgtg ttcgaatggg agcgtgcgat ccgccagaca
      961 gtggtgctgg atctggaaat ggctgccgac gtggcgcgcg cagccgcaac cgatcgtctg
     1021 gaggatgccc tggactataa ggcggtgacc aaccgtgtgg tccagttcgt ggaggacagc
     1081 cgtttccagc tggtggaaac cctggcggaa tccattgcca ccatcctgcg ggacgaattc
     1141 ggcgtgccct ggctacggtt gcgggtgagc aaacctggcg cgatccacgg agcgaaggat
     1201 gtgggcgtgc tgatcgagcg gggtggggct ggttgagtac caccgttttc ctgggcctgg
     1261 gcagcaacga tcagccgcgg cggaatatcc gcctggcgct ggatctgctg caggagtgct
     1321 tcggagccct gcaggtttcg ccggtatttg ccaatgcgcc gctggcgacg gcggcggtag
     1381 cgcccgatgc cgagctgtat ttcaatctgg tggtgtcagc ggtctgcaac gtgccggcgg
     1441 gagagctgaa gcagcagttg cgacggatcg aggaacgctg cgggcgcgtg catgcctcgg
     1501 gcgattgcct ccagaaaaat ccggtgtgtt cgctggatat tgatctgctg ttgtatggcg
     1561 acgcggtggg cgatgtggac ggtgtcgtgc tgccgcatcc ggatgtgctc cggcgcgcct
     1621 atgtcctcta tccactttcc ctgctggtgc cgtccggccg ccatccgcaa ctgcaacaga
     1681 cctttgccga gttgtggcgt ggcttcggca aatccagatg ggaactgaaa ccggtggcgc
     1741 cgtttctgcc ggattattcc ctgcaacagt agtgcccctt cgagtggtgc ctcttccctg
     1801 ctgcagtctc cattgcgctg cgcgaatcct gtcaactcag cgaatatccg ccatccactg
     1861 caatgatctg gccggtgacg aagggcgcct gctccgcaag aaaataaatg gccttggcga
     1921 tgtcatccgg cgagccgatg gtttgcatgg gcgtgttggt cagaatggct tgctgcacgg
     1981 cgtccggcat gttgccgtcg gcgggccaga gaatggtgcc gggggatacg ccattcaccc
     2041 gcacgtccgg tgccagtgcg cgggccagcg acagcgtcat ggctttcagc gcggctttcg
     2101 ccatgcagta gggcgtgaaa tccggcagcg gtttgtcggc attgatatcg acgatgttca
     2161 ccacacatcc gcgactctgt ttcagcagag gcaataaatg ctgggtgaga aaaaacggcg
     2221 ccatggcatt gctggcgaac agatcgttcc actggctgag ggtggtgttg ccaaattccg
     2281 ttgggtagta cgaagaggcg ttattcacca gcagatcgag ccggtgtttc cagctgcgca
     2341 cctgttccgc cagctgttcc caggcggcgg ggtcggacag atccgcatgc agcgcaatgc
     2401 aggagtgggg gcgtaccgaa ttgagccggt cgcgcagatt tttcgcgccg tcgccggaag
     2461 tgcggtaatg cagcacgatg tcgtagtcgc gctgatggaa atagtctgca gtgtgtgcgc
     2521 caatgcgacg ggccgagccg gtgatcaggg ccaccttgcg ggaagaatcg gttgtcattg
     2581 ttattccttc actgttcttt cacgtagcag ttttttacat cgcgaatcgc aacgatgcgg
     2641 cgcttgcgca attcgatgcc aagagtttcg cccttgaaac ccagttccac cagcggtttg
     2701 gcttccaccc ggcgtgcggc gacggcgcaa tcgcgaatat agtcggcttg cggatagtcg
     2761 cgatcttcca ggccggtgcg gccgcgggca tccgcttcgc aggccagcag aaacagcggt
     2821 aatgtctcgg gccgccgcag tgcatccagt gcttccagca ggcgcatcac cgtggccggt
     2881 ttcagttcga aggcgcgatg gcagtgggtg tgaaattctg ccaccagcag gccgagacgt
     2941 ttcaccggat tgggcgcgcg caggcgcagg cacaattttt ccgccaggtg tgcgctgatg
     3001 ggctcgtggc ccggatgact cggccatttg aattccggcg tcatgccctt gcccagatcg
     3061 tgacagatca gggaaaacag cacagcgacg cgatccgcct tttccagcgg gtgagtctgc
     3121 atcagtttca gcgctgtctg caacgtcagc agcacatgaa ggccggtgtc gatttccgga
     3181 tggtgtttgg gcggttgcgg cacaccaaac agtttgtcca gttcgggaaa cactacggcc
     3241 agcgcgccgc agtcgcgcag ggcttcgaaa tagcgccagg gcgaaggttc cagcagggct
     3301 ttttcggttt cggtccacac ccgttccggc gtcagcgtct gcaattcgcc actggcggcg
     3361 atgtcgcgca tcagtgccag cgtttcgtcc gcaatttgaa agccgcgatc gtgaaaacgc
     3421 gccagaaagc gtgccacccg caacacccgc aggggatctt ccacgaaggc gggggacacg
     3481 tggcgcaaca agcggttctg cagatcctgc tggccatgga agggatcaac gagggtgc
//
LOCUS       DASUEG010000876         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1867417, whole genome shotgun sequence.
ACCESSION   DASUEG010000876 DASUEG010000000
VERSION     DASUEG010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867417"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1068
                     /locus_tag="VFX11_16535"
     CDS             196..1068
                     /locus_tag="VFX11_16535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4940282.1"
                     /translation="MTPAPATATESAITRTNASPDAIPNIKVRRMDFEFPDTLPEFWF
                     DSNPVLTALLTAMSVSFPSGERYFIDSVRHFQARIDNAELKEQIRGFIGQEANHTKEH
                     IALNRFMDRKGYPATAMENFVRKAITWVQRRSSPEENLARTAALEHFTAIIAGGFLDH
                     PELFEKMAPELAQIWAWHSIEEVEHRSVAFDVYKSEVNDEKLRRRTMVKVTFIFTMVN
                     SVRTLMLLNVSGHLFNVSAWLKGLNLMWGRAGVFRKLVPQYFAYYRKDFHPAQHDYHA
                     QVEAAKLRYLGDKA"
     gene            complement(1100..1936)
                     /locus_tag="VFX11_16540"
     CDS             complement(1100..1936)
                     /locus_tag="VFX11_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940283.1"
                     /translation="MTAVKTVVITGGNTGIGLATAKQIVQQGQRVILACRDQARAERA
                     RQEIAQAVRGAEVLVYALDLASFDSIRAFAAKLTQDYPAIDVLINNAGVYPTTQQFTR
                     EKFELQFGVNYLGHFLLTHLLLPALKNAGDARIVHLSSIMHNLGKMDFDSFRGRKRYS
                     ATGAYAQSKLANLLFSNELARRLPASITSNAVHPGGVDSEIYRDMPVFLYRILKLFLI
                     SADRAGKFVADMALAESWNGKTGQFKSAHGPLPVARASRNTELSRRLYEESCGLTGVS
                     PL"
     gene            2254..>3272
                     /locus_tag="VFX11_16545"
     CDS             2254..>3272
                     /locus_tag="VFX11_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4940284.1"
                     /translation="MSNRRKNEWWHRSVPVIYPQTYLSLAGERGAEPDSILRDAGLPA
                     DLLANPRAEISFLQMKALVDKVLERTGDDGIGVEVGWRLPPTAFGNLGYGLLCSATLQ
                     DALDLCLRFWHLPGRVVRASLSRSGATCTIDFVLIAPVPEPNHHLILESTLASLYRGF
                     QLLAGASAADLEVWFQGPPPAYADRVSARLGQVRYNMPATQFRFSAHLLQTPLPMHNP
                     TGLIFAIQQCEREESLLNEIENPIREKVRRNLVLGSGGYPSLQKMSEILSMTTRTLRR
                     RLEEEGIQYKTMVEETKRRDAVRLLDDEVMEIQQVAQLLGYQDPANFTRAFRQWTGQT
                     PSQYRA"
BASE COUNT          688 a         1002 c          952 g          630 t
ORIGIN      
        1 aatgatgcac aagaagaaat agttggggca gcggccagct ggtcatgctg ccaaactggt
       61 tgtgcagaat gggcctgacg cagcgttctg tccgcagatg acaatcaatc tgtccctcta
      121 cagcctgacc cggcgggcag cgtcccggtt aacgtagaac ctaccgctca gctgctgaaa
      181 gagaaggagg acactatgac ccctgccccg gccactgcaa cagaatctgc aatcacccgc
      241 accaatgcca gccccgatgc catccccaac atcaaagtca ggcggatgga ttttgagttt
      301 cccgacaccc tgccggaatt ctggttcgac agcaatcccg ttctgaccgc attgctcacg
      361 gccatgtcag tttcctttcc ctctggcgag cgctacttca ttgacagcgt gcggcatttt
      421 caggcgcgca tcgacaatgc cgaactgaaa gaacagattc gcggtttcat tggccaggaa
      481 gccaatcaca ccaaggagca tatcgcgctg aaccggttta tggaccggaa gggctacccg
      541 gccacggcga tggagaattt tgtgcgcaag gccatcacgt gggtgcagcg gcgctccagc
      601 cccgaagaga atctggcgcg caccgccgcg ctggaacatt tcacggccat catcgcgggt
      661 ggctttctcg accatccgga actgttcgaa aaaatggcgc cggagctggc tcaaatctgg
      721 gcctggcatt ccatcgagga agtggagcac cgatccgttg ccttcgatgt gtacaagtca
      781 gaagtgaacg acgaaaaact gcggcggcgc accatggtga aagtgacgtt cattttcacc
      841 atggtgaaca gcgtgcgcac gctgatgttg ctgaacgtct ccggccatct gttcaatgtc
      901 agcgcctggc tgaagggact caatctgatg tgggggcgtg ccggcgtatt tcgcaaactc
      961 gtgccgcagt atttcgcgta ttaccgcaag gattttcatc ctgcacagca cgattatcac
     1021 gcccaggtgg aggcagcgaa attacgctac ctgggggaca aggcgtaata aacgcagcag
     1081 gcgcggaatc caacccgcat cacagcggac tgacccccgt cagtccgcaa ctttcctcat
     1141 acaggcgccg gctcaattcc gtattccggc tcgcgcgcgc caccggcaag gggccgtgcg
     1201 ccgatttgaa ctggcccgtc ttgccattcc agctttccgc cagcgccatg tccgcaacaa
     1261 atttgccggc ccggtcagca gaaatcagaa acagtttcag gatccggtac aggaacactg
     1321 gcatatcccg gtagatttcc gaatccacac cgccgggatg cacggcattg ctggtgatgc
     1381 tggccggcaa ccgccgcgcc agttcgttgc tgaacagcag attcgccagt ttcgattgcg
     1441 catacgcgcc ggtggccgag taacgcttgc ggcccctgaa actgtcgaaa tccattttgc
     1501 ccaggttgtg catgatggaa gacaggtgaa cgattctggc atcgcccgcg ttcttcagcg
     1561 ccggcagcag cagatgcgtc agcaggaaat gccccagata attcacgcca aactgcagct
     1621 cgaatttttc ccgggtgaac tgctgcgttg tgggatacac gccggcgttg ttgatcagca
     1681 cgtcgatggc cgggtagtcc tgcgtcagct ttgcggcaaa cgcgcggatg ctgtcgaacg
     1741 acgccaggtc cagcgcatat accagcactt ccgcgccacg taccgcctgt gcaatttcct
     1801 gcctggcccg ctccgccctt gcctggtccc ggcacgccag aatcacccgc tgaccctgct
     1861 gtacaatctg cttggcggtg gccaggccaa tgccggtatt gcctccggtg ataaccacgg
     1921 ttttcacggc tgtcattgca ttcaccttga cgactggatg agattgggaa ggcggggatc
     1981 atcaccaaaa aatttggccg atgcaacggg gcgtagcatc ggccaatgcg gcttcaacac
     2041 caccggtggt cctcactggt cctgggggat gcatcggtac ggcaggaagc cggagcgccc
     2101 attattcctg ccaggggaca gtgtgatgag attccaaccg gacaaaatac ttgtcatctg
     2161 cggccagtgc gccacgctgc aacctgcccg atactggcgt gcagctgtat cgcgacggaa
     2221 cggtaccgga aaacacatgg ggacaatagc cgggtgagca acaggcgcaa gaacgaatgg
     2281 tggcaccgca gcgtgcctgt catctatccg cagacttatc tgagcctggc cggtgaacgc
     2341 ggtgccgagc cggacagcat tctccgtgac gccggcctgc ccgccgatct gctggccaat
     2401 ccccgggcgg aaatttcctt cctgcagatg aaggcgctgg tggacaaggt gctggaacgc
     2461 accggcgatg acggtatcgg cgtcgaggtg ggctggcgcc tgccacccac ggcgttcggc
     2521 aatctcggtt acggattgtt gtgcagcgca accctgcagg atgcgctgga tctgtgcctg
     2581 cgcttctggc atttgccggg gcgggtggtg cgggcctcgc tcagccgcag cggcgccacc
     2641 tgcaccattg atttcgtcct tatcgcgccg gtgccggaac ccaatcacca cctgatcctt
     2701 gaatccaccc tggccagcct gtatcgcggt tttcaattgc tggcaggtgc cagcgctgcc
     2761 gatctggagg tctggttcca gggcccgccg cccgcctacg ccgaccgggt cagcgcgcgg
     2821 ctcggccagg tgcgctacaa catgccggct acgcaattcc gcttcagcgc acacctgctg
     2881 caaaccccgc tgcccatgca caatcccacc ggattgatct tcgcgatcca gcagtgcgag
     2941 cgggaagaat ccctgctcaa cgaaatagaa aatcccatcc gcgaaaaagt gcggcgcaat
     3001 ctggtgctgg gcagtggcgg ctatccatcg ttgcagaaaa tgagtgaaat tctgagcatg
     3061 accacccgca cgctaaggcg ccgcctggaa gaggaaggca tccagtacaa gaccatggtg
     3121 gaagaaacca agcggcgcga tgcggtgcgg ctgctggatg acgaggtcat ggaaatccag
     3181 caggtggcgc agctgctggg gtatcaggac cccgccaatt tcacccgcgc gttccgtcag
     3241 tggaccgggc agactcccag ccagtaccgc gc
//
LOCUS       DASUEG010000877         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_592125, whole genome shotgun sequence.
ACCESSION   DASUEG010000877 DASUEG010000000
VERSION     DASUEG010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592125"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFX11_16550"
     CDS             complement(<1..187)
                     /locus_tag="VFX11_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX4940285.1"
                     /translation="MRYTKTPYAQHQFIDTDGKAVGDLPPWMENSEQLLRFYRTMTLI
                     RLFDKRVVALQRTGQMGT"
     gene            complement(205..1227)
                     /locus_tag="VFX11_16555"
     CDS             complement(205..1227)
                     /locus_tag="VFX11_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HEX4940286.1"
                     /translation="MFDKIGQAHLSELHFKSDEAAQLQAIIAIHNTKLGPALGGCRCI
                     EYASDDAAITDAIRLARGMSYKAALAKVPQGGGKAVILKPKQAFDRAALYRAFGKFVN
                     ELGGRYITAVDSGTHLDDMDEVARITPYVSGSNIDGLDPSPMTALGVLAGMKAAAFHR
                     YGNADLAGRIVAIQGVGNVGFALAELLHKEGARLLVSDIDPLKVDQVKRAFGAEAVNP
                     EHFFHVHCHILAPCGLGAVINDATLTQLDCDIIAGSANNQLAEPRHGQILHERGILYA
                     PDYVINAGGLIKVSLGHLQRSESAIRTKTWGIGETLLHMFERAAADNRPPSQVADQMA
                     EDILYH"
     gene            complement(1502..>3087)
                     /locus_tag="VFX11_16560"
     CDS             complement(1502..>3087)
                     /locus_tag="VFX11_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HEX4940287.1"
                     /translation="KMVVAPEGEYQTFITRKKDLPQEIPANWRVTPIDSDRVTIQLYE
                     STAIFLPDHQPFPVRAAGQLPSGFEPGASYAARQHPRGLEMAVYAATDAIRSVGIPWQ
                     DILNQIAPDKISVYASAAMSQLDENGVGGMLRAGLMGKRTSSKQCPLGFAEMPADFIN
                     AYVLGNVGSTGAMMGACATFLYNLDKAIQDIQSGKALIAIVGTSDAPILPEVMEGYRV
                     MGALAEDEGLKKLDGISTDPDYRRACRPFSDNCGFTIGESAQYVVLCNDTLALQLGAS
                     VYGAAPAVFVHADGIKKSISAPGMGNYLTLGRAVHFARQIGSTPRALQHSFVMAHGTG
                     TPQNRVTESHVLDSVAEAFGIAEWPVAAVKAYLGHSIGSAGGDQLAAALGVFAQGILP
                     GIKTITHFAADIHRQHLSLDIQHREYANGHFEQAILNAKGFGGNNASAVLLSPQRTTA
                     MLQQRHGKHDWLDYLHRNESVREQQQQYHDQLLREIPASIYRYGEPVVDGHELKITSN
                     HIQIPGYNHPVDLATPNPY"
BASE COUNT          586 a          884 c          946 g          671 t
ORIGIN      
        1 aggttcccat ctggccggtg cgctgcagcg ccaccacccg tttgtcgaac aggcggatca
       61 gcgtcatggt gcgataaaac cgcagcaact gttcactgtt ctccatccag ggcggcagat
      121 cgccgacggc ttttccgtcc gtatcgatga actggtgttg tgcataaggc gttttcgtat
      181 accgcatttt attgttcccc acgctcagtg atacagaatg tcttccgcca tctgatcggc
      241 gacctgtgat ggtggccggt tgtcggctgc cgcccgttcg aacatgtgca gcagggtttc
      301 gccgatgccc caggtcttgg tgcgaatggc gctttcgctg cgctgcagat ggcccaggga
      361 aaccttgatc aggccgccgg cgttgatcac ataatccggc gcgtacagga tgccgcgttc
      421 atgcaggatc tggccgtggc ggggttccgc cagctgattg ttggccgagc cggcgatgat
      481 gtcgcaatcc agctgggtga gggtggcatc gttgatcacg gcgcccaggc cgcagggtgc
      541 caggatgtgg caatggacgt ggaaaaaatg ctcggggtta acggcttcgg caccgaaggc
      601 gcgtttcacc tgatcgactt ttagcgggtc gatgtcactg accagcaggc gcgcgccctc
      661 cttgtgcagc aactccgcca gcgcaaatcc cacgttgccc acgccctgga tcgcgacgat
      721 gcggcccgcc agatcggcgt tgccgtagcg gtgaaaggcg gcggctttca taccggccag
      781 tacgcccagt gcggtcatgg gggacggatc gagcccgtcg atattgctgc cggatacata
      841 gggcgtaatg cgcgccacct catccatgtc atccagatgg gtgccgctgt ccaccgccgt
      901 gatatagcgg ccaccgagtt cattcacgaa tttgccgaag gcgcgataca gcgcggcgcg
      961 gtcgaatgcc tgtttgggtt tgaggatgac ggccttgccg ccaccttgtg gcacctttgc
     1021 cagcgcagcc ttgtagctca tgccgcgcgc caggcggatg gcatcggtga tggcagcgtc
     1081 gtccgatgca tattcgatgc agcgacagcc gcccagtgcc gggcccagtt tggtgttgtg
     1141 gatggcgatg atggcctgca gttgcgcggc ttcatcggat ttgaaatgca gttcgctgag
     1201 gtgcgcctgc ccgattttgt cgaacatggt actactcctt ccaaacgagg gtggggtaac
     1261 acccgtgtgc gttcttccgg tgctgtgaag aattctggat cgcagaatgg cttcaccaac
     1321 aggacgtatt ttttacggtc tgttctaaag atataagaaa aattcggatt tgacgaatcg
     1381 ggcgcggggt ttttctgtat ccaatgggcg tcagattttt tccgcgacac tgaaaagccg
     1441 tctaaccact tgatatcatt ttcgttatca agacgtcaga cggccgttca cgccatttga
     1501 atcaataggg attgggcgtg gccagatcta ccggatggtt ataaccgggg atctgaatat
     1561 gattgctggt aatcttcagt tcatgaccat ccaccacggg ctcgccgtaa cggtagatgg
     1621 aggccggaat ttcccgcagc aactgatcat gatactgctg ctgttgctcc cgcacggatt
     1681 cgttgcggtg caggtaatcc agccagtcat gcttgccatg gcgttgctgc aacatcgcgg
     1741 tagtgcgctg cggtgacagc agcacggccg atgcgttgtt gccaccaaaa cccttggcgt
     1801 tgagaatcgc ctgttcgaaa tgaccgttcg cgtattcgcg gtgctgaatg tcgagtgaca
     1861 gatgctggcg gtgaatatct gctgcgaaat gcgtgatggt tttaatgccg ggcagaatgc
     1921 cttgtgcaaa cacacccagt gccgccgcca gctgatcacc gccagcagag ccaatggagt
     1981 ggcccaggta cgccttgacg gccgcaacgg gccactcggc aatgccaaag gcttccgcca
     2041 cgctgtccag cacgtgggat tcggtgacgc gattctgcgg cgtgccggtg ccgtgggcca
     2101 tcacgaacga atgctgcagt gcgcgcggag tggaaccgat ctgccgggcg aaatgcacag
     2161 cacggcccag ggtcagatag ttgcccatgc cgggtgcgga aatggatttc ttgatgccgt
     2221 ccgcgtgcac gaatactgcc ggcgcggcgc cataaacgct ggcacccagt tgcaacgcca
     2281 gcgtgtcatt gcacagcacc acatattgtg cggattcgcc gatggtgaag ccgcaattat
     2341 ccgagaaagg acgacaggca cggcgatagt ccggatcggt gctgatgcca tcgagtttct
     2401 tcagcccttc gtcttccgcc agcgcgccca tcacgcgata gccttccatg acttccggca
     2461 gaatcggtgc gtcgctggtg ccgacaatgg caatcagcgc cttgccgctc tgaatgtcct
     2521 ggatcgcctt gtccaggtta tacaaaaacg tggcgcaggc acccatcatc gcgccggtgg
     2581 aaccgacatt gcccagcacg taggcattga tgaagtctgc cggcatttcc gcaaagccca
     2641 gcgggcattg tttcgacgac gtgcgcttgc ccatcaggcc cgcgcgcaac atgccgccga
     2701 caccgttttc atccagctgg ctcatggcgg cgctggcgta gacgctgatt ttgtccggcg
     2761 cgatctggtt cagaatgtcc tgccagggaa tgccgacgga acggatcgca tcggtagcgg
     2821 cgtacacggc catctccagg ccgcgcggat gctgacgtgc ggcatagctg gcgcccggct
     2881 cgaaaccgga aggcagctgg cctgcggcgc gcacgggaaa cggctggtga tccggcagaa
     2941 atatggcagt gctttcatac agctggatcg taacgcggtc gctgtcgatc ggtgtcacgc
     3001 gccagttggc gggaatttcc tgcggcagat cctttttgcg cgtgatgaag gtttggtact
     3061 cgccttccgg cgccaccacc atcttgc
//
LOCUS       DASUEG010000878         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1933768, whole genome shotgun sequence.
ACCESSION   DASUEG010000878 DASUEG010000000
VERSION     DASUEG010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933768"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /gene="moaA"
                     /locus_tag="VFX11_16565"
     CDS             complement(<1..943)
                     /gene="moaA"
                     /locus_tag="VFX11_16565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX4940288.1"
                     /translation="MNLYTLASPDSAVAPAAPELRDRFGRRIDYVRVSVTDRCDLRCS
                     YCIPKGFKDFEEPAHWLTFDEMERLLGVFVRLGVRRVRLTGGEPLLRRGLPDLARRLS
                     ALGVDDLSLSTNGTQLRHQAHALRQAGVQRLNVSLDSLRRECVEQLSGRDSLQQVLDG
                     LQAAQVAGFDRIKINMVVMATTPDADIDAMVDFCRQHAFVLRLIEAMPMGDSGRSAGT
                     VDLQQVARRLRQRFDLHETAQPLGAGPARYLSSADGDFSVGFITPMSQHFCATCNRVR
                     LAVDGTLYLCLGQEEKLELGPLLRGGASDSELETAIRQ"
     gene            complement(1041..1433)
                     /locus_tag="VFX11_16570"
     CDS             complement(1041..1433)
                     /locus_tag="VFX11_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX4940289.1"
                     /translation="MKNRKPASACACGSGLAQAQCCEPLIKGERPAPTAEALMRSRYV
                     AYALHADDYVLATWHPSTRPPALNADEVPIKWVGLEVHAHHPDGERAQVEFTACYKVQ
                     GRLARLHEVSRFVFEQGRWWYVDGDHPS"
     gene            complement(1452..>2081)
                     /locus_tag="VFX11_16575"
     CDS             complement(1452..>2081)
                     /locus_tag="VFX11_16575"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEX4940290.1"
                     /translation="LYFETLRQGVAMEDAAIIEELVYALQQYGDHPAFLRHDNKPVLF
                     IWASGRVTAERWKTILAAVRQQAGPALFIGMGCDTLDLDVFDGVHDYTVNAPTDLPRY
                     QRLCGNKTRHHFLLSPSGGRKLWAATAMPGYDDRALTQRAEHLLIPRDSGAYYRHTLQ
                     AALDSQPDWLLVTSWNEWGENTHIENSVVYGDLYERLTREYTAPWLANR"
BASE COUNT          407 a          670 c          638 g          366 t
ORIGIN      
        1 cctggcgaat ggcagtctcc agttcactgt cgctggcgcc gccgcgcagc aggggcccca
       61 gttccagttt ctcttcctga ccgaggcata gatacagggt gccatccacg gccagtcgca
      121 cgcggttgca ggtggcgcag aaatgttgcg acatgggtgt aatgaagccg acgctgaaat
      181 cgccatctgc gctgctcagg taacgcgcgg gcccggctcc cagcggctgc gccgtctcat
      241 gcaggtcgaa acgctgccgc agacggcggg ccacttgctg cagatccact gtgccggcgg
      301 agcggccact gtcacccatg ggcatggcct cgatcaggcg caggacgaaa gcatgctggc
      361 ggcaaaaatc gaccatggca tcaatatccg catcgggtgt tgttgccatg accaccatat
      421 tgatcttgat ccggtcgaaa ccggccacct gggccgcctg caaaccgtcc agcacctgct
      481 gcagactgtc acggccggaa agttgctcga cacactcgcg ccgcagcgaa tccagactga
      541 cattgaggcg ctgcacgccc gcctgacgca gggcgtgcgc ctgatggcgc aactgtgtgc
      601 cattagtgga cagcgacagg tcgtccaccc caagcgcact gagacgccgc gccaggtctg
      661 gcaggccccg gcgcaacaag ggttcgcccc cggtcaggcg cacgcgccgt acccccaggc
      721 gcacgaacac gccgagcagg cgttccatct cgtcgaaggt aagccagtgc gcgggttcct
      781 caaagtcttt gaagcctttg ggaatgcaat agctgcagcg cagatcgcag cgatcggtta
      841 cggatacgcg cacataatcg atgcggcgac cgaaacggtc gcgcaattct ggggcggccg
      901 gagcgaccgc ggagtcaggg gaagccaggg tgtacaggtt catgcgcatt ctctagcaag
      961 acttgttccc cccgccggaa cggcacagac gcggcgggta cctgtgcaat cagtcgcagc
     1021 aagcggcccc gccttacagc tcaggatggg tgatcaccat cgacatacca ccatcgcccc
     1081 tgttcaaaca caaaacggct tacctcgtgc aggcgcgcca gccggccctg caccttatag
     1141 caggcggtaa actcgacctg cgcccgctct ccgtcggggt ggtgcgcatg cacttccagt
     1201 ccgacccact tgatgggcac ctcgtcggca ttcagcgccg gcggacgcgt ggacggatgc
     1261 caggtcgcca gcacatagtc atcggcatga agcgcgtagg ccacatagcg tgagcgcatc
     1321 agcgcttctg ccgtaggtgc cggtcgctcg cccttgatca gcggttcgca gcactgcgcc
     1381 tgcgccaggc cgctgccaca cgcacaagcg ctagcgggtt tgcggttttt catgcgcgac
     1441 gttccttcct ttcagcgatt cgccagccat ggcgcggtgt attcacgtgt caggcgctca
     1501 tacaggtcgc cataaacgac gctgttttca atatgggtgt tttcgcccca ctcattccag
     1561 ctggtcacca gcaaccagtc gggctgacta tccagcgccg cctgcagggt atggcggtag
     1621 taagcgccgg aatcacgggg gataagcaaa tgttccgcgc gctgcgtgag ggcccggtca
     1681 tcataacccg gcatggccgt ggcggcccac agcttgcgcc cgccggacgg gctgagcagg
     1741 aagtggtggc gggtcttgtt gccgcacaga cgctgataac gcggcaaatc ggtcggagca
     1801 ttgacggtgt aatcgtgtac gccatcaaac acatccagat cgagcgtatc gcagcccatg
     1861 ccgataaaca gcgccgggcc ggcctgctgg cgcacggcgg ccagaatggt tttccagcgc
     1921 tctgccgtga cgcggccgct ggcccagata aacaacaccg gcttgttgtc gtggcggaga
     1981 aaagccgggt gatcgccata ctgctgcagg gcatacacca gctcttcgat aatggcggca
     2041 tcttccatgg ccacaccctg acgcagggtt tcgaaataca g
//
LOCUS       DASUEG010000879         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1982195, whole genome shotgun sequence.
ACCESSION   DASUEG010000879 DASUEG010000000
VERSION     DASUEG010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982195"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFX11_16580"
     CDS             complement(<1..788)
                     /locus_tag="VFX11_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940291.1"
                     /translation="MLNPYFTEDHNLLREQIRRFVENEIVPNGEAWEEDGCVPREILR
                     KMGDLGFLGLTHEEQYGGSGMDVRGAVVLAEELSRSTFAGVNITALVHTNMASPHLAN
                     AGSPEQKQKYLPDIIAGKKITAICVTEPGAGSDVAGIRTRAVQDGNDWVINGSKIFIT
                     NGVYGDLYFVAAKTDPTAKGSRGISMFIVEKGTPGFKVGRALKKHGWRSSDTAELFFD
                     DVRVPQANMLGELNKGFYAVMKNFQHERIMLAAMGVGEAQKALD"
     gene            complement(811..2877)
                     /locus_tag="VFX11_16585"
     CDS             complement(811..2877)
                     /locus_tag="VFX11_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4940292.1"
                     /translation="MSVTHATGGIKRLLIANRGEIACRVIRSCKKLGIESVAVFSDAD
                     RRARHVQQADLACHIGGAAVGESYLNAKAIIDAALKVKADAIHPGYGFLSESTQLISL
                     CNQNGIIFVGPTLESIAAMGSKIEAKEIAQGLEIPTVPGYNGETQDADRLQKEAERIG
                     FPVLIKASAGGGGKGMRIVRQSSEFREALTLARQEALNAFGDDRVLLEKYLVRPRHIE
                     VQLLGDTHGNVVHLFDRECSIQRNYQKVIEEAPVSHLDMKVRQSLFDCAVRLGRHLGY
                     RGAGTVEFIFDADSQQAYFLEMNTRLQVEHPTTELVTGIDLVEWQIRVANGEALAFAQ
                     QDLKTIGHAIEARVNAEDPANNYLPEIGTIQLYREPVAEGLRIDSGVQQGSEITPFYD
                     SMLAKVIAFGDTRGLASQRLLHGLNNFAIGGVKTNQEFLTSLITRPAFHQELTTQYIG
                     KEFPQGWQPWGEQMHTAIAACAFALEQEHRTDAAMAGTPWQKLGGWRLTAGAGTPAVA
                     RLWLQTNNGLREIRVVGRQGKYDVQVIEPAAKDTPSTSAHFAIHLYRPAVTLTDAQWQ
                     VELDGTRRSLDIAITEKQVSISSPRLRRSYRFIGLEDAMLGQAAATKNAGNVIRARMP
                     GLITDVLVQVGDLVEEGATAVVMEAMKLIHNMPCPVSGTVKQVLVAAGDKVEDGSLLI
                     EIEPAQ"
     gene            complement(2874..3674)
                     /locus_tag="VFX11_16590"
     CDS             complement(2874..3674)
                     /locus_tag="VFX11_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4940293.1"
                     /translation="MSSYSSFNTIKVSVDARGVATLLLNRPERHNAMNDELIREVTEA
                     AVLLDADPKVRCVILTGTGESFCAGGDLKWMQGILNSSRAERVADSAKLAKMLRTLNE
                     LSKPLIGRINGQAYGGGTGLVSVCDIAIAADTANMALTEVRLGLTPANISTFVVGKMG
                     RANARRTFLNARPLTAAKAKEVGLVDEVVPAADLDAAVETEVAYVLQCGPGAIAATKK
                     LIFYVDSHDTDTNLIYTANLLADTWESAEGQEGIRCFFNKDVPSWRQP"
     gene            complement(3703..>4372)
                     /locus_tag="VFX11_16595"
     CDS             complement(3703..>4372)
                     /locus_tag="VFX11_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4940294.1"
                     /translation="IDASDFQEFKPQWGTSLICGTAHIQGYPVGILGNNGPLFSDASV
                     KGAHFIELCEQRNIPLLFLHNVPGFMVGKAAEQGGISKHSAKLVYAMSCAKVPRFSVV
                     IGGSYGAGNYGMCGRGFRPNLMFAWPNSVVATMSADIGTNVVMELARTSITKPPTPEA
                     LDKLDRETRAMYAEKSGAYYATSRLWDDGIIEPEQTRDVIGLCLAVAATLPPEEGRSY
                     VYRM"
BASE COUNT          785 a         1308 c         1329 g          950 t
ORIGIN      
        1 atgtcgagcg ccttctgtgc ttcgccgacg cccatggcag ccagcatgat gcgttcgtgc
       61 tggaagtttt tcatgacggc gtaaaatcct ttgttaagct cgccgagcat gttggcctgg
      121 ggtacgcgca cgtcgtcgaa aaacaactcg gcggtgtcgg aactgcgcca gccgtgtttc
      181 ttcagggcgc gtccgacctt gaagccgggt gtgccttttt cgacgatgaa catggatata
      241 ccgcgggagc ctttggcggt gggatcggtt ttggcggcga cgaaatagag gtcgccataa
      301 acgccattgg tgatgaagat tttgctgccg ttgatgaccc agtcgttgcc gtcctgcacg
      361 gcacgtgtgc gaatgccggc gacgtcggag ccggcgcccg gttcggtgac gcagatggcg
      421 gtgatttttt tgccggcgat gatgtcgggc aggtatttct gtttttgctc cggtgagccg
      481 gcgttggcga ggtgaggcga ggccatgttg gtgtgaacca gcgcggtgat gttaactccg
      541 gcaaaggtgg agcgggacag ttcttctgcc aggacgacgg cgccgcgcac gtccatgccg
      601 ctgccgccgt actgttcttc gtgggtgagg ccgaggaagc caagatcgcc cattttgcgc
      661 aggatttcac gggggacgca gccgtcttct tcccaggctt cgccgttggg gacgatttcg
      721 ttttccacga agcggcggat ctgttcgcgc aggaggttgt ggtcttcggt gaaatagggg
      781 ttcaacattt tgttttggcc tcgtgaattt ttattgcgcc ggttcaatct cgatcagcaa
      841 gctgccgtct tccaccttgt ctccggcggc gaccaatacc tgtttgacgg taccgctgac
      901 cgggcacggc atgttgtgaa tcagcttcat cgcttccatc accacggcgg tggcgccctc
      961 ttccaccaaa tcgccgactt gcaccagcac atccgtgatc aggccgggca tgcgcgcgcg
     1021 aatgacatta ccggcattct tggtagccgc cgcctgaccc agcatggcgt cttccagtcc
     1081 gatgaaccgg taggaccgac gcaggcgcgg cgagctgatc gatacctgct tttcggtaat
     1141 ggcgatgtcg agtgagcggc gggtgccgtc cagttccacc tgccactgcg cgtcagtgag
     1201 ggtgactgcg gggcggtaca ggtgaatggc gaaatgcgct gatgtggaag gggtgtcctt
     1261 cgccgcaggt tcgattacct gcacgtcgta tttgccttgc cggccaacga cgcggatttc
     1321 gcgcaagcca ttgttggttt gcagccacag ccgcgcgacg gccggtgtac cggcaccagc
     1381 tgtcaaacgc cagccgccca gtttctgcca gggtgtgccc gccatggcgg cgtcggtgcg
     1441 gtgttcctgt tccagcgcga acgcgcacgc tgcaatggcg gtgtgcatct gctcgcccca
     1501 gggctgccag ccctgcggaa attccttgcc aatatattgc gtggtcaatt cctggtgaaa
     1561 cgcggggcgc gtgatcaggc tggtaagaaa ttcctgattc gttttcacgc cgccgatggc
     1621 gaaattgttc agaccatgca gcaggcgttg cgatgccagt ccgcgcgtgt caccaaaggc
     1681 gatgactttt gccagcatgg aatcgtagaa cggggtgatt tccgagccct gctgcacacc
     1741 gctgtcgatg cgcaatcctt ccgccactgg ttcgcggtac agctggatgg tgccgatttc
     1801 cggcaggtaa ttgttggcgg ggtcttccgc gttcacccgg gcttcgatgg catggccaat
     1861 ggtcttcaga tcctgttgcg cgaatgccag tgcttcgcca ttggcgacgc ggatctgcca
     1921 ttccaccaga tcgatgccag tgaccagttc ggtggtggga tgctccacct gcaggcgggt
     1981 gttcatttcc aggaaatagg cctgctggct gtcggcgtcg aaaatgaatt cgacagtgcc
     2041 ggcaccgcga tagcccagat gccggcccag acgcacggcg caatcgaaca ggctctggcg
     2101 caccttcatg tccagatgac tgaccggcgc ttcctcgatg accttttgat aattgcgctg
     2161 gatcgagcat tcgcggtcga acaggtgcac cacgttaccg tgggtgtcgc ccagcaactg
     2221 cacttcgatg tgacggggcc gcaccaggta tttttccagc agcacacggt catcaccgaa
     2281 cgcgttcaat gcttcctgcc gcgccagtgt caacgcttcg cggaattcgc tggactgccg
     2341 cacgatgcgc atgcccttgc caccgccgcc ggcgctggcc ttgatcagca cggggaagcc
     2401 aatgcgctcc gcttccttct gcaggcgatc ggcatcctgg gtttcgccgt tgtagcccgg
     2461 caccgtggga atttccaggc cctgcgcgat ctccttggct tcgattttcg aacccatggc
     2521 ggcgatgctt tccagcgtcg gaccgacaaa aataatgccg ttctggttgc acagggaaat
     2581 cagttgcgtg ctttccgaca ggaaaccata accgggatgg atcgcgtcgg ctttcacttt
     2641 cagcgcggca tcaatgatgg ctttcgcgtt caggtagctt tcgcccacgg ccgcaccgcc
     2701 gatatggcag gccagatccg cctgctgcac gtgacgggca cgacgatccg catcggaaaa
     2761 tacggccacc gattcgatcc cgagtttctt gcagctgcga atcacgcggc aggcgatctc
     2821 gccccggttg gcgatcagca ggcgcttgat gccgccggtt gcatgggtaa cgctcatggc
     2881 tgcctccagc tcggcacatc cttgttgaag aagcagcgga tgccttcctg cccttccgcc
     2941 gattcccagg tgtcggccag cagattggcg gtatagatca ggttggtgtc ggtgtcgtgg
     3001 ctgtcgacat agaaaatcag cttcttggtg gcggcgatgg caccaggccc gcactgcagc
     3061 acataggcga cttccgtttc cactgcggca tccagatcgg ctgccggcac cacttcatcc
     3121 accagcccca cttccttcgc tttggctgcc gtgagcggac gcgcattcag aaaagtgcga
     3181 cgggcgttgg cgcggcccat cttgcccacc acgaaggtgg agatgttggc gggtgtgagg
     3241 cccagacgca cttcggtcag tgccatattg gcggtgtccg ccgcgatggc gatatcgcac
     3301 accgacacca gtcccgtgcc acccccgtaa gcctgaccat tgatgcggcc gatcagcggc
     3361 ttcgacagtt cattcagggt gcgcagcatt tttgccagct tggcactgtc agcgacgcgt
     3421 tcggcgcggc tggaattgag aatgccctgc atccatttca ggtcaccgcc ggcacagaaa
     3481 gattcgccag taccggtgag aatcacacag cgcactttcg gatcggcatc cagcagcacg
     3541 gcggcttcgg ttacttcgcg aatcagctca tcgttcatcg cgttgtggcg ctcggggcgg
     3601 ttcagcagca acgtggccac accacgtgcg tccacagaaa ccttgatcgt attgaaactc
     3661 gaatagctac tcatcattca attccttcaa cagcaaaacc ggtcacatgc ggtaaacgta
     3721 agaacggcct tcttccggcg gcaaggtggc cgccaccgcc agacacaacc cgatcacgtc
     3781 gcgggtctgt tccggttcga tgatgccgtc atcccacagg cgcgaggtgg cgtaataggc
     3841 gccgctcttc tcggcgtaca tggcgcgggt ttcgcggtcc agtttgtcca gtgcttccgg
     3901 tgtcggcggt ttggtgatgc tggtacgcgc cagctccatc accacgttgg tgccgatgtc
     3961 ggcgctcatg gtggccacca cggaattggg ccacgcgaac atcaggttgg ggcggaaacc
     4021 gcggccacac atgccgtagt tgcccgcgcc gtaagacccg ccgatcacca cgctgaagcg
     4081 cggcactttc gcgcacgaca tggcatacac cagcttggcg gaatgtttgg aaataccgcc
     4141 ctgctccgcc gccttgccca ccatgaagcc cggcacgtta tgcaggaaca gcagcggaat
     4201 attgcgctgc tcgcacagtt cgatgaaatg ggcacctttc accgacgcat cggaaaacaa
     4261 cggcccgttg ttgcccagaa tgcccaccgg gtagccctgg atatgcgcag tgccacagat
     4321 caggctggtg ccccactggg gtttgaattc ctggaagtcg ctggcgtcga ta
//
LOCUS       DASUEG010000880         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1447511, whole genome shotgun sequence.
ACCESSION   DASUEG010000880 DASUEG010000000
VERSION     DASUEG010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1153
                     /locus_tag="VFX11_16600"
     CDS             161..1153
                     /locus_tag="VFX11_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940295.1"
                     /translation="MPNADLPIMIVDDAKFSGAVIGRTLKSAGYTDVRTASSAQQALL
                     MLESRPVNVLIADWLMPEMDGLELASRVRQLDEATNHFTYIMLLTAREGDQALMQAFD
                     QGVDDFINKSAMNQQLLPRIYAADRLAGLQNRLLKENQLLVESNQRLKKNSLVDPLTG
                     LGNQQFALERLQATIQHAQSRGGAACLMIINIDNYNTLMQQHGKIILREIMVGISRRL
                     RQLVRPLDVVARLSNEEFAVITHQPDIGQANAVTYRRVYDALNVKALKTSKGFVQLKA
                     AMCMSAADTETGFPAPDKMLGITKQQIEQARTIGRLMVIKWRSVKKPEGDTPAP"
     gene            1172..2113
                     /locus_tag="VFX11_16605"
     CDS             1172..2113
                     /locus_tag="VFX11_16605"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4940296.1"
                     /translation="MPHADWHILGPGAIGSLFAWHLAEAGSSVRLLTRHPGESARLIT
                     LDDHTASPHTSATRAFHVDHQDDTPIRRLLVTVKAHQTTAALESIRHRIDSRSLILFL
                     QNGMGTWDCAKTLFPATRWLVGTTTEGAWRAADRHIVHAGRGETWIGSLNPAWTNDAE
                     QLVDTLKNTRFHPGHDAQILQRLWRKLAVNCAINPLTVLFDCRNGELLQKPAALRQME
                     AVCVEVERVMGAALGGVTVEPLFPLVRTVAEKTGANHSSMLQDYRRGQPTEIEFITGY
                     LVAEGRRLGIDTPENAGILDAIRGLNPHWSGTDCTLP"
     gene            complement(2160..2447)
                     /locus_tag="VFX11_16610"
     CDS             complement(2160..2447)
                     /locus_tag="VFX11_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940297.1"
                     /translation="MSNVLINTELLRELLNAASRTAMTHRGSEHESYVLGQLEATANF
                     AYVLAAGSGDAELELLCQQLALDALNRYSELEYKALTAAAGGDTRRACPQY"
     gene            2683..3243
                     /locus_tag="VFX11_16615"
     CDS             2683..3243
                     /locus_tag="VFX11_16615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX4940298.1"
                     /translation="MGHRLSKIYTRTGDKGTTGLGDGTRVDKDCLRVEAYGTTDELNS
                     AIGMVLSEATVPDDLRSWLLEIQHDLFDLGGELCIPGHIVLNATFIDRLEHQIDAMNE
                     HLPPLKEFILPGGNRAASTCHLARTICRRAERRVLSLAKQEPINDWGLKYLNRLSDFL
                     FVAARVLARTDNGSEVLWNRQRFSQP"
     gene            3240..>3517
                     /locus_tag="VFX11_16620"
     CDS             3240..>3517
                     /locus_tag="VFX11_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX4940299.1"
                     /translation="MSDFITGIDFILGGARSGKSVMAEQRARASGKRIVYIATAEARD
                     GEMQNRIAHHRDRRPSHWQTVEEPLHLAQALRAHQSPDHLILVDCLTL"
BASE COUNT          730 a         1160 c         1045 g          582 t
ORIGIN      
        1 ctggctgcga ccgcggatca aggccgccgc agctgaaaat tgcgacacgg atacagacga
       61 atcgcctccg gatcagcgat aatctgggcg caattagaca gagccgcagc gctcggccac
      121 acttaaaaac aataatcagg cccacaacaa gaatcccact atgcccaatg ccgatttgcc
      181 catcatgatc gtggatgacg cgaagttttc cggcgccgtg attggacgaa ccctgaaatc
      241 cgccggctac accgatgtcc gcaccgccag cagcgcccag caggccctgc tgatgctgga
      301 aagccgcccc gtcaatgtgc tgatcgccga ctggctgatg ccggaaatgg acggcctgga
      361 gctggccagc cgtgtgcgcc agctggacga agccaccaat cacttcacct acatcatgct
      421 gctcaccgca cgggaaggtg atcaggcgct gatgcaggcg ttcgaccagg gcgtggacga
      481 tttcatcaac aaatccgcca tgaaccagca gttgctgccg cgcatttatg ccgccgaccg
      541 cctggccgga ttgcagaacc gcctgctgaa ggaaaaccag ctgctggtgg aatcgaacca
      601 gcgcctgaaa aagaattccc tggtggaccc gctcaccggc ctgggcaacc agcagttcgc
      661 gctggagcgc ctgcaggcca ccatccagca cgcccagtcc cggggcggcg ccgcctgcct
      721 gatgatcatc aacatcgaca actacaacac cctgatgcag cagcacggca agatcatcct
      781 gcgcgagatc atggtgggta tctcccgccg cctgcgccag ctggtgcggc cgctggacgt
      841 ggtggcgcgg ctgtcgaacg aggaattcgc cgtcatcacc caccagcccg acatcggcca
      901 ggccaacgcg gtgacctacc gccgcgtcta cgatgcgctg aacgtgaaag cactgaaaac
      961 ctcgaaaggt tttgtgcagc tgaaagccgc catgtgcatg tcggcggcgg acaccgaaac
     1021 cggttttccc gcgccggaca aaatgctcgg catcaccaaa cagcagatcg agcaggcgcg
     1081 taccatcggc cggttgatgg tgataaagtg gcgcagcgtg aaaaagcccg agggcgacac
     1141 gccggccccg taacccacca ccggaattcc catgccgcac gccgactggc acattctggg
     1201 cccgggagcc atcggctccc tgttcgcctg gcacctggcg gaagccggca gcagtgtccg
     1261 cctgctcacc cgccatcccg gtgaaagcgc ccgtctgatc accctggacg accacaccgc
     1321 ctcgccccac accagcgcca cccgggcctt ccatgtggat caccaggacg acacccccat
     1381 ccggcgcctg ctggtgaccg tgaaagcgca ccagaccacc gccgccctgg aatccatccg
     1441 tcaccgcatc gattcccgca gcctgatcct gttcctgcag aacggcatgg gcacctggga
     1501 ctgcgccaaa accctgttcc ccgccacccg ctggctggtg ggcaccacca ccgaaggtgc
     1561 ctggcgcgcg gcggatcgcc acatcgtcca cgccgggcgc ggcgagacct ggatcggcag
     1621 cctgaacccg gcctggacca acgacgcgga acaactggtt gatacgctga aaaacacccg
     1681 gttccacccc ggccatgatg cgcagatcct gcaacggctg tggcgcaagc tggcagtgaa
     1741 ctgcgcgatc aatcccctca cggtgctgtt tgactgccgc aacggcgagc tgctgcagaa
     1801 gccggcggcg ctgaggcaga tggaagcggt gtgtgttgag gtggaacggg tgatgggagc
     1861 tgcgctgggg ggcgttacgg tggaaccgct gttcccactg gtgcgcacgg tggcggaaaa
     1921 gaccggcgcc aatcattcat cgatgctgca ggactaccgg cggggccagc ccaccgagat
     1981 cgaattcatt accgggtatc tggtggcgga aggtcggcgt ctgggcatcg acacacccga
     2041 gaacgccggg attctggatg ccatccgggg gctgaatccg cactggtcag gcactgattg
     2101 cacgctcccc tgagcagact aagggccggg tatcacacgt aacggtatcg aactggagat
     2161 cagtattgag ggcaggcccg acgggtatcg ccgcctgcag cggcagtcaa ggccttgtat
     2221 tccagttcgg aataacggtt cagggcatcc agcgccagtt gctggcacag cagctcaagt
     2281 tccgcatcac cagatccggc ggccagcaca taggcgaaat tggcagtcgc ctccagctgg
     2341 cccaggacat aggattcatg ctcgctgccc cggtgggtca tggccgtacg tgacgcggcg
     2401 ttcaacaatt cccgcagtaa ttcggtattg atcaggacat tgctcatggt cggcttctcc
     2461 gggacagaat gcttcaacgt gggtgtcaga ctgcggtcac gtcagcttga ccctcacaaa
     2521 gcttagtcgt agattgccgc tccattctgt gacttttgat acaaaagagg gggcgaaaag
     2581 gccatttttt gatcaaaatc agactgtcac gaacttgctg gtcacggggc gccaaagcca
     2641 gtcgccttaa cctgaaatcc gaacctgaac tggagccatc ccatgggcca ccgcctttcc
     2701 aaaatctaca cccgcaccgg cgacaaaggc actaccggac tgggggacgg cacccgggtg
     2761 gacaaggact gcctgcgggt ggaggcctat ggcaccaccg acgaactcaa ctccgcgatc
     2821 ggcatggtgc tgtcggaagc caccgtgccg gacgatctgc ggtcctggct gctggaaatc
     2881 cagcacgacc tgttcgatct ggggggtgaa ctctgcattc cgggccatat cgtgctgaac
     2941 gccaccttta tcgaccggct ggaacaccag atcgacgcca tgaatgaaca tctgccgccg
     3001 ctgaaggaat tcatcctgcc gggtggcaac cgcgccgcca gtacctgcca cctggcccgc
     3061 accatctgcc gtcgtgccga gcgccgggtg ctgtcgctgg ccaagcagga gcccatcaat
     3121 gactggggcc tgaaatacct gaaccggctg tcggactttc tgttcgtggc cgcccgcgtg
     3181 ctggcccgca ccgacaacgg cagtgaagtg ctgtggaacc ggcagcgctt ttcccagcca
     3241 tgagcgactt catcaccggc atcgatttca ttctcggggg cgcccgctcc ggcaagagcg
     3301 tgatggcaga gcagcgggcc cgggccagcg gcaagcgcat cgtgtacatc gccaccgccg
     3361 aggcccgcga cggcgagatg cagaaccgca tcgcccatca ccgcgaccgg cgcccgtccc
     3421 actggcagac ggtggaagaa cccctgcacc tggcccaggc cctgcgcgcg caccagtcgc
     3481 ccgaccacct gatcctggtg gattgtctga ctctgtg
//
LOCUS       DASUEG010000881         4802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1646656, whole genome shotgun sequence.
ACCESSION   DASUEG010000881 DASUEG010000000
VERSION     DASUEG010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646656"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFX11_16625"
     CDS             <1..407
                     /locus_tag="VFX11_16625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX4940300.1"
                     /translation="EASKPLTDAFAKVGIDCIFLIAPTTKIPRIEKIARAAGGYLYYV
                     SVKGVTGAGNLDVEEVNRKLETIRKLTDLPITVGFGIKDAATARLIGEKADGVVVGSA
                     LVNLVAQNQNDLPALQSRIAELMQSLRQGLDQ"
     gene            432..1298
                     /gene="accD"
                     /locus_tag="VFX11_16630"
     CDS             432..1298
                     /gene="accD"
                     /locus_tag="VFX11_16630"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX4940301.1"
                     /translation="MSNSWIEKILPSVGRNTNKKTNIPEGLWRKCAKCGAFLYRPELE
                     KNLDVCPKCDHHMRLSARRRLDLFLDQDGRSEIAADLEPVDRLKFKDSKRYKDRLVAA
                     QKGTGEKDVLIVLKGKVNDYPVVASAFEFEFMGGSMGSIVGEKFVRGVNAAIAARTPF
                     VCFSASGGARMQEALFSLMQMAKTSAALEKLKEARLPFISVLTDPVYGGVSASLAMLG
                     DINVAEPNALIGFAGPRVIEQTVRQKLPEGFQRSEFLLEHGALDMIVHRLQLKDTLHR
                     LMAKLLPAATAA"
     gene            1298..2641
                     /gene="folC"
                     /locus_tag="VFX11_16635"
     CDS             1298..2641
                     /gene="folC"
                     /locus_tag="VFX11_16635"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX4940302.1"
                     /translation="MTDTPSLQAAGQGLVTSTVTLTTLSQWLQRLERLHFKSIDMGLE
                     RVSRVAQALGLLSGLPFIFTVGGTNGKGSTVAFLESILRAGGYRVGTYTSPHLIDFNE
                     RVRIDGSEVSDEQLIAAFEKIESARNDISLTYFEFTTLAALLVFRQAQLDVLVLEVGL
                     GGRLDAVNVWDADVAVVTSIDLDHQAFLGNTREKIGLEKIGIGRAGKPLVLGERNTPQ
                     LVLDSLRALAIPFLQLGRDFDYQTRENDWRFAGLSRDSKPCVQEPLPVPVLYIANAAL
                     ALQALALSPFTPAPDAIRAGLENATLAGRQQIVQHQPDLMLDVGHNPHAAAHLARRLE
                     KSAYRRVHCIVGMLRDKALADTLVELLPVVDCWYPVTLGGERGQGADAIASELREAGA
                     RVGFLAASPAQACRRLLTQVQRDDLILVFGSFYTVADVIQCRARTAGTRIVEKRT"
     gene            2646..3320
                     /locus_tag="VFX11_16640"
     CDS             2646..3320
                     /locus_tag="VFX11_16640"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4940303.1"
                     /translation="MEQNLKQRLVGALVLISLAVIFLPLVFDGQQQRIDTTQYAIPEQ
                     PALTLKAVDIEPLEQQAAEELAVVNTITQQKQEQEEQAATQMPADATTNIPAAQPQSP
                     VAPAPAPATPTTQPASPVATYIEQEKKIDQALQNAPPGQTVDLAEAWIIQVGAFSSQP
                     NADGLRDKLKANGYPAFVKPVKVASGSLFKVYVGPEIRRHRVDQQKLELEQKYQLKAL
                     ILKYIP"
     gene            3399..3899
                     /locus_tag="VFX11_16645"
     CDS             3399..3899
                     /locus_tag="VFX11_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX4940304.1"
                     /translation="MTIADYVILGSIAVSMVLSLLRGFTIEAISLASWIAAFVIARLF
                     SLPFAVLLNDLVDPPSMRQPVAFILLFVLTLIVGALIKHLAKAFINATGLSGTDRLLG
                     MVFGAARGALLVVVSLSVLSRTTQMPADPWWQKSVLIPHFLLVEGWTAEMGQKAWNGI
                     MAIGRT"
     gene            3959..>4802
                     /locus_tag="VFX11_16650"
     CDS             3959..>4802
                     /locus_tag="VFX11_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX4940305.1"
                     /translation="MCGIVGIVGRSNVNQTLYDALTVLQHRGQDAAGMVTCDQDKLFL
                     RKDNGMVRDVFRTRHMRRLQGNMGIGHVRYPTAGTSSSAEAQPFYVNSPYGITLAHNG
                     NLTNAGEIGEDLYKTDLRHINTNSDSEVLLNIFAHELQVLGKLVPTEEDIFSAVSAVH
                     RRCKGAYAVVAMITGYGIVGFRDPYGIRPACFGERETESGIEYMIASESVALQAMGFQ
                     LIRDIAPGEAVYINMEGQLFTRQCASNPVYRPCIFEHVYLARPDSIMDGISVYKARLR
                     MGERL"
BASE COUNT          961 a         1384 c         1463 g          994 t
ORIGIN      
        1 cggaagcgtc caagccactg accgacgctt tcgccaaggt cggcatcgac tgcatcttcc
       61 tgatagcgcc cacaaccaaa atcccacgca tcgagaagat tgcgcgggcc gcgggcggtt
      121 acctgtatta cgtgtcggtg aagggcgtaa ccggagcggg gaatctggat gtggaagagg
      181 tgaaccgcaa gctggaaaca attcgcaaac taacggattt gcccattacc gtgggctttg
      241 gtatcaagga tgccgcaacc gcgcgcctga tcggcgaaaa ggccgatggc gtggtggtgg
      301 gcagcgcgct ggtcaatctg gtggcgcaaa accagaacga ccttcctgcc ctgcagtccc
      361 gcattgccga actcatgcaa tccctgcggc agggtctgga ccaataataa ttcttaactc
      421 aggatggcat tatgagtaac agctggattg agaaaatcct gcccagtgtt gggagaaaca
      481 caaacaagaa gaccaatatc cccgaaggac tctggcggaa atgcgccaaa tgcggtgcgt
      541 ttctctatcg tcccgagctg gaaaagaatc tggatgtctg ccccaagtgc gatcaccaca
      601 tgcggttgag tgcgcggcgc cggctggacc tgtttctgga ccaggacggc cgtagcgaga
      661 tcgctgccga tctggagccg gtggatcgcc tgaaattcaa ggacagcaag cgttacaagg
      721 atcgtctggt tgccgcgcaa aagggcactg gcgagaagga cgtgctgatc gtattgaaag
      781 gcaaggtcaa tgattatccg gtggtggcgt ctgcctttga attcgaattc atgggcggct
      841 ccatgggttc catcgttggc gagaaattcg tgcgtggcgt gaacgcggca attgctgccc
      901 gaaccccgtt cgtgtgtttt tctgccagtg gtggtgcgcg catgcaggaa gcactgttct
      961 cgctgatgca gatggcgaaa accagtgccg cactggaaaa gctgaaagaa gcccgcctgc
     1021 cgttcatttc cgtgctgacc gatccggttt acggtggcgt gtccgccagt ctggccatgc
     1081 tgggtgatat caatgtggcg gagcccaatg cgctgatcgg ttttgccggc ccccgcgtga
     1141 ttgagcagac cgtgcgccag aaattaccgg aaggtttcca gcgcagtgaa ttcctgctgg
     1201 agcatggcgc gctggacatg atcgtgcacc gcctgcagtt gaaagacacg ctgcaccgtc
     1261 tgatggcaaa actgttgccg gcggcaacgg cggcctgatg accgataccc cgtcgttgca
     1321 ggccgccggt caggggttgg tgacgtccac agtgacgttg acgactctca gccagtggct
     1381 gcaacgactg gagcgtctgc atttcaaatc catcgatatg ggtctggagc gggtgagccg
     1441 ggtggcgcag gcgctgggcc tgctctctgg tctgcccttc atcttcacgg taggcggcac
     1501 caacggcaaa ggctccaccg ttgcgtttct ggaatccatt cttcgcgccg gcggttatcg
     1561 cgtcggcacc tacacgtccc cccatctgat cgatttcaac gagcgcgtgc gcattgacgg
     1621 cagcgaggta tcggatgaac agctgatcgc tgcgtttgaa aaaatcgagt ccgcccgcaa
     1681 cgacatcagt ctcacttatt ttgaatttac caccctcgcg gcgttgctgg ttttcaggca
     1741 ggcgcaactc gatgtgctgg tgctggaagt ggggctgggt gggcgcctcg atgctgtgaa
     1801 tgtgtgggat gcagatgtgg cggtggtgac ctccatcgat ctggaccatc aggcgtttct
     1861 tggcaatacc cgcgaaaaaa tcggcctgga gaaaatcggc atcggccgcg ccggcaagcc
     1921 gctggtgctg ggagagcgca atacgccgca actggtgctg gattccctgc gggcactggc
     1981 gattcccttt ctgcagctgg ggcgtgattt cgattatcag acgcgggaaa acgactggcg
     2041 ctttgcggga ctgtcgcggg acagcaaacc ctgtgtgcag gaaccactgc cggttccagt
     2101 gctgtacatc gccaatgccg ctctggcgct gcaggcactg gcgttaagtc cattcacacc
     2161 agcgcccgat gccatccggg ccggtctgga aaatgccact ctggccggac gccagcagat
     2221 cgtgcagcac cagcccgacc tgatgctgga tgtgggccac aatccccatg cggctgccca
     2281 tctggcccgc cggctggaga agtcagcata ccgccgcgtg cactgcatcg tcggcatgct
     2341 gcgggataaa gcgctggcgg acactctggt ggaactgctg ccggtagtgg actgctggta
     2401 cccggtcacc ctgggtggcg aacgggggca gggcgccgat gccattgcgt cggaattgcg
     2461 cgaggccggc gcccgggtag gctttctggc ggcgtcccct gcacaggcct gtcgccggct
     2521 gttgacgcag gtacagcgcg atgacctgat tttggtcttc ggttccttct atactgtcgc
     2581 agacgtaata caatgccgcg ctcggacggc aggcacccgg atcgtagaaa agaggaccta
     2641 aggctttgga acaaaacctg aaacaacggt tggtgggcgc actggtgctg atctcgctgg
     2701 cggtgatttt tctgccactg gtattcgacg ggcagcagca gcgcatcgac accacccaat
     2761 atgccatacc cgagcagccc gcgttgacgc tgaaagcagt ggacatcgag ccgctggaac
     2821 agcaggccgc tgaggaactg gcggtagtca ataccatcac ccaacagaag caggaacagg
     2881 aagagcaggc ggcaacccag atgcctgccg atgccaccac caatatacct gcagcccagc
     2941 cgcaatcgcc cgttgctccc gctcccgctc ccgcaacccc gaccacccag cctgcttccc
     3001 ccgtcgcgac ctacatcgag caggaaaaga aaatcgacca ggcactgcaa aacgctccac
     3061 cggggcagac cgtggatctg gcggaagcct ggatcatcca ggtgggcgcc ttttccagcc
     3121 agcccaacgc cgatggcctg cgggacaagc tcaaggccaa cggctatccg gcgtttgtga
     3181 agccggtgaa ggtggcatcg ggcagtctgt tcaaggtgta tgtgggccct gaaatccgcc
     3241 gccaccgggt ggatcagcag aaactggagc tggaacagaa ataccaactc aaagcgctga
     3301 ttttgaagta cattccctga acagaatggc gtaggatttt tgtccgcctt tttggtagaa
     3361 tgcgcgctct tcccaggacc cggtcagtat ttcctacgat gactattgcc gattatgtga
     3421 ttctcggttc catcgccgtt tccatggtgc tgagtctgct gcgcgggttt accatcgaag
     3481 ccatttctct agccagctgg attgccgcat ttgtcattgc ccgtctgttc agtttgccgt
     3541 tcgccgtcct gctcaacgat ctggttgatc cgccttccat gcggcagccg gtggcattca
     3601 tattgttgtt cgtgctcacc ctgattgtcg gcgccctgat caagcatctg gccaaggcgt
     3661 tcatcaatgc caccgggctg tcgggcaccg accgtctgct gggcatggta tttggcgctg
     3721 cgcgaggcgc attgctggtg gtggtgagtc tgtccgtgct gtcacggacc acccagatgc
     3781 cggccgaccc ctggtggcag aagtccgtat tgatacccca tttcctgctg gtggaaggct
     3841 ggaccgcaga aatggggcag aaagcctgga acgggatcat ggctatcggc cgtacctgat
     3901 cctgcttgcg ctatcattgt tgttcatcgc agttcatgac cgatcgaggt aatagcgaat
     3961 gtgcggaata gtcggcatcg ttggccgctc caacgtcaat caaaccctgt acgacgcctt
     4021 gacggtgctg cagcaccgtg gccaggatgc cgctggaatg gtcacctgtg atcaggacaa
     4081 actgttcctg cgcaaggaca atggcatggt gcgggatgtg ttccgcaccc gtcacatgcg
     4141 ccggctgcag ggcaacatgg gcatcggcca tgtgcgctat cccacggcgg ggacttccag
     4201 ttctgcggag gcacagccct tctacgtgaa ctccccgtat ggcatcacgc tggcccataa
     4261 cggcaacctg accaatgcgg gggagattgg cgaggatctg tacaaaaccg atctgcgcca
     4321 cattaacacc aactccgatt cggaagtgct gctgaacatt tttgcgcacg agctgcaggt
     4381 gctgggcaaa ctggtgccca ccgaggaaga cattttttct gcggtttctg ccgtgcatcg
     4441 tcgctgcaag ggtgcctacg ccgtagtggc catgatcacc ggctacggca tcgtcggctt
     4501 ccgcgatccc tacggcatcc gccccgcctg cttcggcgag cgtgagaccg aaagcggtat
     4561 cgaatacatg attgcgtcgg aaagtgtggc gctgcaggcc atgggcttcc agctgatccg
     4621 cgacatcgcg ccgggcgagg cggtgtacat caacatggaa gggcagctgt tcacccgcca
     4681 gtgtgcgtcc aatccggtgt accgtccctg cattttcgag catgtgtacc tggcgcggcc
     4741 ggattccatc atggatggca tttccgtgta caaggcccgc ctgcgcatgg gcgagcggct
     4801 gg
//
LOCUS       DASUEG010000882         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1206911, whole genome shotgun sequence.
ACCESSION   DASUEG010000882 DASUEG010000000
VERSION     DASUEG010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206911"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFX11_16655"
     CDS             complement(<1..622)
                     /locus_tag="VFX11_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4940306.1"
                     /translation="MIQIHRENWPVLVAAARTPFLESAGAYSELMSYELGACALRGLM
                     QRVALDPQLIELVVMGTVLHEINTTNVAREAMLSAGLPPSIPAYTVAMAGISPSVGVM
                     NICNEIALGRMQFGIAGGTENFSDVPIRLSQKIRRTLMKVRQSRDSRERVKLLAQLRP
                     WDLKLDLPMSADYTTGLSMGSACEAMVRTFGASRADSDAFTAHSHTA"
     gene            complement(619..1515)
                     /locus_tag="VFX11_16660"
     CDS             complement(619..1515)
                     /locus_tag="VFX11_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4940307.1"
                     /translation="MPTTVKDLPFRLLLEHRDTISAMALGGLRSVLPFKPKQAVPTPV
                     HRTVNPPSAELLKAYRDWCQAAILQGIPPHFVCAKISMPVVAELTAQSPYPLLSVLNQ
                     GVLLQLHQPLPEGEPIELRGELVDASDDGFRARIHSRVQVGTPSAPHAITIDAIAAVM
                     LKKRPGGDADKKRAEPDYVTIDHWQAQWNEGQTFFWLTGDFNPIHTVPALAKRTRFKG
                     CIMHGYGAFAQVYEALQRSGARFQDIEARFVRPLPLPSGPLLIQRAQTPDEDGQIAFR
                     LLDEAGTLYQAGRYLPVAEARP"
     gene            complement(1725..2279)
                     /locus_tag="VFX11_16665"
     CDS             complement(1725..2279)
                     /locus_tag="VFX11_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX4940308.1"
                     /translation="MSKIGIFFGTETGTTRLIAKKLHKLLGDDIADKPVNVNRITPDD
                     MLQYDSLILGTPSYGVGEIPGLSAGCLERNWEEFLVQLHNPDFSGKRIAFFGLGAQER
                     YADRFASSLMALVQVFKGFGADIVGDWSTEGYTFQHSASVVDGRFVGLVIDHRTQGMY
                     TDERLKIWAEQIRPLLSAKRASAA"
     gene            complement(2394..>4646)
                     /locus_tag="VFX11_16670"
     CDS             complement(2394..>4646)
                     /locus_tag="VFX11_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX4940309.1"
                     /translation="PPTKHQQYLNEAKALDLAEMKQLKANKRTALVVVLIRHQYAQTL
                     DSAADIVIKTLRKMEHSAEKRLEEYRADQQKQIDRLISVLSGTVKVYLDKPESVAAFD
                     PILGKEGKNILALCEQYMAYAGNNYYPFMVPLYKKQRPALFRAVEILKLEAATEDTDV
                     LKAFEFICLHKQRRTETLPIQENPDDPNSKNLLNIRWIRDKWWKLVTGKATKSAQITH
                     VNKLAFELCVFDRIAEELSTGDLYIPYSETFDDYREQMISWEQYDTELPGYCEELGLV
                     KGSTEFTQALKTQLTEACYAADRNFPDDEQVRIENGKLIIGKSKAEEASREIEAISEL
                     LQDRLEKINLLDLIINAERWLSLHKLFGPLSGFESRVDDPFLRFVLTLFCYGTNIGPT
                     ETERSVRGISRKQVAWLNLKRTTVERLDKAIFKVSNAYKKFNLIECWGSGNSVSADGK
                     LWDLYEDNLLSEYHLRYGRFGGIAYYHVSDTYIALFSHFIPCGVYEAIYILDGLLNDE
                     SDFKPDTVHGDTQAQSTPVFGLAYLLGIKLMPRIRNIKDLNFYKPDRNMVLQHIQSLF
                     HEPIQWDRIEKYYPDMLRTTMSIKAGKITASTILRRFGTKNRKNKLYFAFRELGRVVR
                     TMFLLEYITDLELRKTIQAATCKSEEFNEFARWLFFANSGKIASNLKHEQGKIVKFNH
                     LLANLAILYNVNAMTTAFNDLRAEGHDIRREHMAKFSPYHTAHLGRLGSFELDLNKEV
                     KPMRVKLEIE"
BASE COUNT          949 a         1308 c         1311 g         1078 t
ORIGIN      
        1 cggcagtatg gctgtgggca gtgaaggcgt cgctgtcggc ccggctggcg ccaaaggtgc
       61 ggaccatggc ttcgcaggcc gatcccatac tgagcccggt ggtgtaatcc gcgctcatgg
      121 gcaaatccag tttcaaatcc cacggacgca gctgcgccag taacttcacg cgctcgcggc
      181 tgtcgcggct ctgccgcact ttcatcaggg tgcggcggat tttctgcgac agccggatcg
      241 gtacgtcgga aaaattttcc gtgccgccgg cgataccgaa ctgcatccgg cccagggcga
      301 tttcattgca gatgttcatc acgcccacac tgggagaaat tcccgccatg gccacggtgt
      361 acgcgggaat cgacggtggc aagcccgcac tcaacatggc ttcccgtgca acgttggtgg
      421 tgttgatttc gtgcagcacc gtgcccatca ccaccagttc aatcaactgc ggatcgaggg
      481 caacgcgctg catcaggccg cgcagtgcgc acgcgcccag ttcataactc atcaattccg
      541 aataggcgcc ggcactttcc agaaaaggcg tgcgggcggc ggccaccaga acaggccagt
      601 tttcccggtg aatttgtatc atgggcgggc ctccgcaacg ggaaggtaac ggccggcctg
      661 gtacagggtg ccggcttcgt ccagcaggcg aaaggcaatc tggccatcct catccggtgt
      721 ttgcgcccgc tggatcagca gcggaccgct ggggagcggc agcggccgta caaaacgcgc
      781 ttcgatatcc tggaatcgcg caccgctgcg ctgcagcgcc tcgtatacct gcgcgaaggc
      841 gccgtagccg tgcatgatgc agcctttgaa gcgggtgcgt ttcgccagcg ctggcaccgt
      901 atgaatcgga ttgaaatcgc cggtgagcca gaagaaggtc tggccttcat tccactgcgc
      961 ctgccagtgg tcgatggtga cgtaatcggg ttccgcgcgt tttttgtcgg cgtcgccgcc
     1021 agggcgcttc ttcagcatga cggcggcaat cgcatcgatg gtgatggcat ggggtgccga
     1081 cggcgtgccc acctgcacgc gggaatgaat gcgcgcccgg aaaccgtcgt cgctggcgtc
     1141 caccaattcg ccccgcaatt cgatgggttc accctccggc aatggttgat gcagttgcag
     1201 aagcacgccc tggttcagca cgctcagcag cgggtagggt gattgcgcgg tgagttccgc
     1261 caccaccggc atgctgattt tggcgcagac aaaatgcggc gggatgccct ggaggatggc
     1321 ggcctggcac cagtcacggt aagccttcag cagctcggca gagggcggat tcacagttcg
     1381 gtgcacgggt gtcggcaccg cttgtttggg tttgaacggc aggacggaac gcaggccgcc
     1441 cagggccatg gcgctgatgg tgtcccggtg ctccagcagc aggcggaagg gcaggtcttt
     1501 cacggttgtc ggcatggtct ggcgctcgca cggaagttga ccgggcaagc atgcctgaaa
     1561 acgccgctcg cgataagtca cagcgttgac ttcaggcgtg caaaaagtga caggcgtggt
     1621 tgtggcacgg cttttctccg tttgcaccgc cactgtctga agccaattag ccagccgaga
     1681 aagcacggcg ccgggttggc tggaggtgat cctatttccg cgcgtcaggc tgccgatgcg
     1741 cgctttgcac tgagcagcgg ccgaatctgt tccgcccaga ttttcaggcg ctcgtcggtg
     1801 tacatgcctt gcgtgcgatg atcgatcacc agccccacga aacgcccgtc caccaccgag
     1861 gcggaatgct ggaaggtata accctcggtg ctccagtcgc ccacgatatc ggcgccgaag
     1921 cctttgaaca cctgaaccag tgccatgagc gaactggcga agcgatccgc atagcgttcc
     1981 tgcgcaccca gcccaaaaaa cgcaatgcgc ttgccggaaa aatccggatt gtgcagttgc
     2041 accaggaatt cctcccagtt gcgctccagg cagccggcgc tgagccccgg aatctcgccc
     2101 actccatagc tgggcgtgcc gaggataagg gaatcgtact gcagcatatc gtccggcgtg
     2161 atgcggttga cgttcaccgg cttgtcggcg atgtcgtcac ccagcagctt gtgcagcttc
     2221 ttggcaatca ggcgggtagt gccggtttcc gtgccgaaaa aaatgccgat cttgctcatg
     2281 acgtcagccc cgtttgttgg atgtgcgaca acgccccggc ctgcgtagga ggtccggccg
     2341 gtaaaagtgt tggatttagc cacttcgctc ttaactatat gaaatagcta gctttattca
     2401 atttccagtt taacccgcat cggcttcact tccttattta gatccaactc aaagctaccc
     2461 agccggccca gatgtgccgt gtggtacggc gagaacttgg ccatgtgctc ccgcctgata
     2521 tcgtgccctt ccgcacgtag gtcgttgaac gctgtggtca tcgcgttaac gttgtagaga
     2581 atggccaggt tggccagcag gtggttgaat tttacgatct tcccctgctc gtgcttcaga
     2641 ttggacgcga tctttccgct gttggcgaag aacaaccagc gcgcaaactc gttgaactcc
     2701 tcgcttttgc aggttgctgc ctgaatggtt ttgcgcagtt ccagatcagt gatgtactcc
     2761 agcagaaaca ttgtccgcac cacccgaccc agctcacgga atgcaaagta cagtttgttc
     2821 ttgcggtttt tggtgccaaa acgccgcagg atggttgagg ctgtgatctt gcccgccttg
     2881 atcgacattg tcgtccttag catgtcggga tagtacttct cgatgcggtc ccactgaatc
     2941 ggctcatgaa acaaggactg aatgtgctgg agcaccatat tgcgatccgg tttatagaaa
     3001 ttcaaatcct tgatgttacg aatgcgcggc atcaatttga tgcccagcaa atatgccagc
     3061 ccgaatacag gtgttgattg ggcttgggtg tcaccatgta ccgtgtccgg cttaaagtcg
     3121 gactcgtcat tcagcaaccc atccaggata tagatcgcct catatacccc gcacggaatg
     3181 aagtgactga acagcgcaat gtaggtgtcg gacacgtggt aataagcaat gccgccaaac
     3241 cggccgtatc gtagatggta ttctgacagc agattatcct cgtacagatc ccacaatttg
     3301 ccgtctgccg atacgctgtt ccccgatccc cagcactcaa tcagattaaa tttcttgtac
     3361 gcattgctga ctttgaatat ggccttatcc agacgctcaa cggtggttcg cttgaggttg
     3421 agccaagcta cctgtttgcg gctgattcca cgaacggagc gttccgtttc agtgggtccg
     3481 atattcgtgc cataacagaa taacgtcagc acaaagcgca ggaatggatc atccaccctg
     3541 gattcaaagc ctgataaggg gccaaacaac ttatgcaacg acagccaacg ttccgcattg
     3601 atgatgagat ccagcaaatt gatcttttcc aaccgatcct gcagcaactc tgaaattgcc
     3661 tcaatctccc gcgaagcctc ttctgcttta ctcttgccaa tgatcagctt gccgttttca
     3721 atgcgcacct gttcgtcatc tggaaaattg cgatcagccg cgtaacaagc ctccgtcaat
     3781 tgcgtcttga gcgcttgggt gaactcggtg ctacccttca ccagtcccag ctcttcgcag
     3841 taacctggca gctctgtatc gtattgctcc cagctgatca tctgctcccg ataatcatca
     3901 aaggtttcgc tgtaaggaat gtacagatcg ccagtgctca gttcctctgc gatgcggtca
     3961 aatacacaga gctcaaaggc cagcttgttc acatgggtga tctgggcaga tttggtggct
     4021 ttgccagtga ccagcttcca ccacttatcg cgaatccagc ggatattgag gaggtttttg
     4081 ctattgggat cgtcggggtt ttcctggatc gggagcgttt ccgttctgcg ttgcttgtgc
     4141 aggcaaataa attcgaaggc cttcaggacg tcagtgtctt cggtagccgc ttccagcttg
     4201 agaatttcca ccgcgcgaaa taacgctggt cgctgttttt tgtatagggg caccatgaac
     4261 ggataatagt tgttgcccgc ataagccata tactgctcgc aaagcgcgag tatatttttg
     4321 ccttctttgc ccaggatggg atcgaaggcg gcaacggact ctggtttatc caggtagacc
     4381 ttgacggttc ccgacaatac cgagatcaag cgatcgatct gtttctgttg gtctgcccga
     4441 tattcttcca gccgtttttc ggccgaatgc tccattttgc gcaaggtctt gatgacgatg
     4501 tcagcagcgc tgtcgagggt ttgggcgtat tgatgccgta tcaacacaac caccaacgcg
     4561 gtgcgtttgt tggccttgag ttgcttcatt tccgccagat cgagtgcctt ggcctcatta
     4621 aggtattgct gatgcttggt cggagg
//
LOCUS       DASUEG010000883         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1861834, whole genome shotgun sequence.
ACCESSION   DASUEG010000883 DASUEG010000000
VERSION     DASUEG010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861834"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFX11_16675"
     CDS             <1..539
                     /locus_tag="VFX11_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4940310.1"
                     /translation="PIIAVTAHALASEKHQLLNSGMDDYVTKPINESQLIHIIHKWTG
                     VNLHQKNDERGTTSAEPGRNGAAVDMQLGIKLANGKADLAEEMLDMLFTSLQKDKSLI
                     TERLADEDYGALLEVVHKLHGATRYTGVPKLQKCSFALEEALKLQRYETVPELTSDLM
                     QAIDQVVGWYVENRVPVR"
     gene            complement(587..1132)
                     /locus_tag="VFX11_16680"
     CDS             complement(587..1132)
                     /locus_tag="VFX11_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX4940311.1"
                     /translation="MKCTSCKQGSLQPAYLDQLFPCHTCDHCGGNWILLGDYLAWKEA
                     NKDYVFAPEAGEPVAEDHKQALLCPVSGNLMLKYRVSADSDHRLDLSPAVHGMWLDKG
                     EWALLKQKGLAGSLNRIFTDPWQRKIREASARETFQHLYEHEFGAADYAKIQSIRQWL
                     WQHPHKLKLIAYLTDEDPYSA"
     gene            complement(1129..1533)
                     /gene="acpS"
                     /locus_tag="VFX11_16685"
     CDS             complement(1129..1533)
                     /gene="acpS"
                     /locus_tag="VFX11_16685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011262547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX4940312.1"
                     /translation="MIVGIGTDIIAVERMAESFARMGDAYPRRLLTESEFAEFQKRSD
                     INKAAYLAKRFAAKEAAVKALGTGFADGITWKHVNIHNNEKGAPVMTLTGRALEHANT
                     LGVTRVHLSISDEKEHAVAFVILESDVQGVAS"
     gene            complement(1530..2270)
                     /gene="pdxJ"
                     /locus_tag="VFX11_16690"
     CDS             complement(1530..2270)
                     /gene="pdxJ"
                     /locus_tag="VFX11_16690"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX4940313.1"
                     /translation="MNLHNRILLGVNVDHVATLRQARGTRYPDPVQAALDAEEAGADG
                     ITVHLREDRRHIQERDVRILMDTLQTRVNLEMAVTSEMLAYAEFIKPPHCCLVPEKRQ
                     ELTTEGGLDVVGNFDRIKAACDRLGAAGIEVSLFIDADQAQIEAAARAGAPVIEIHTG
                     AYADAETDTALQAEFQRIRAGVDIGLQNGLVVNAGHGLNYQNVEPIAAIPGITELNIG
                     HGIIARAVFTGLKEAVREMKRLMLQARA"
     gene            complement(2400..>3001)
                     /locus_tag="VFX11_16695"
     CDS             complement(2400..>3001)
                     /locus_tag="VFX11_16695"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEX4940314.1"
                     /translation="KEGRQGAKARLLQPFTPLAITFDGKQDLKSGRLLEAAAASVMLT
                     GTALFSGMYLNELLVRLLHKEEALPELFQHYEATLRQLQDMPPEIPLRLFEQNLLLEL
                     GYELVLDRDTEGQPVQRDGHYLYEADSGFVRLLQLPKEPSRLRRCFSGAHLLEIHRQA
                     YEDSAVRAAAKQLSRLALAPHLGDRPLHSRELFQQLAKR"
BASE COUNT          610 a          930 c          834 g          627 t
ORIGIN      
        1 caccaatcat tgccgtgacc gcccatgcac tggccagtga gaaacaccag ctgctcaatt
       61 ccggcatgga tgactatgtc accaagccca tcaacgaatc ccagctgatt cacatcatcc
      121 acaaatggac cggcgtcaac ctgcaccaga aaaatgacga gcgcggtacc acctctgccg
      181 aaccgggccg caatggcgcc gccgtggaca tgcagctggg catcaagctg gccaacggca
      241 aagcagatct ggcggaggaa atgctggaca tgctgttcac gtcgctgcag aaggacaaat
      301 cgctcatcac cgaacggctg gcggacgaag attacggcgc gctactggaa gtggtgcaca
      361 agctgcacgg tgccacccgc tacaccggtg taccgaaatt gcagaagtgc tcattcgcgc
      421 tggaggaggc gctgaagctg cagcgttacg agaccgtgcc cgagctgacc agcgatctga
      481 tgcaggcaat cgatcaggtg gtgggctggt atgtggaaaa ccgggtgccg gtgcgctgaa
      541 ggcaccggcg gccggtcatt gttgtgtgct caacttcgca gccagatcag gctgaatagg
      601 ggtcttcatc tgtcagatag gcgatcagct tcagcttgtg cggatgctgc cacaaccact
      661 gccggatcga ctggatcttt gcataatccg ccgcaccgaa ttcatgctcg tacaaatgct
      721 ggaaggtttc ccgagctgag gcttcgcgaa tcttgcgctg ccagggatcg gtgaaaatcc
      781 ggttcaggct gcctgccaga cctttctgct tcagcaacgc ccattcgccc ttgtccagcc
      841 acatgccgtg caccgcaggg ctcagatcca gccggtgatc cgaatcggcg gacacccggt
      901 atttcagcat caggttgccg gatacaggac acagcaacgc ctgcttgtga tcctccgcca
      961 ccggctcacc tgcttccggt gcaaagacat aatccttgtt ggcttccttc caggccagat
     1021 agtcccccag cagaatccag ttgccaccac aatgatcgca ggtgtggcag ggaaacagct
     1081 ggtccagata ggcaggctgc aggctgccct gtttgcaact ggtgcatttc atgatgcaac
     1141 tccttgtacg tccgattcca gaatgacgaa cgccaccgcg tgctcttttt cgtcggaaat
     1201 tgacagatgc acacgggtga cacccagcgt gttggcatgt tccagcgctc tgcccgtcaa
     1261 cgtcatgacc ggcgcgcctt tttcgttgtt gtgaatgttg acgtgtttcc aggtgatacc
     1321 gtcggcaaat ccggtgccga gagccttcac cgccgcctct ttcgccgcaa accgcttggc
     1381 cagatacgcc gccttgttga tatcgctgcg cttctggaat tcggcaaatt cggattcggt
     1441 gagcagacgg cgcggatagg catcgcccat gcgtgcgaag gattccgcca tccgctccac
     1501 ggcaataatg tcagtgccga taccgacgat catgcccgcg cctgcagcat cagtcgcttc
     1561 atttcacgca ccgcctcttt caggccggta aaaacggccc gggcaatgat tccgtgacca
     1621 atgttcaatt cggtaatacc gggaatagcc gcaatcggtt ccacattctg gtagttcaaa
     1681 ccgtggccgg cattcacgac cagtccgttc tgcaagccga tgtccacgcc ggcgcgaatg
     1741 cgctggaact ccgcctgcaa ggctgtatcg gtttcagcat cggcataggc gccggtgtga
     1801 atttcgatga ccggtgcacc ggccctggcc gccgcttcga tctgcgcctg atccgcatcg
     1861 ataaacaacg acacttcgat tcccgccgcg cccaaccggt cgcaggctgc cttgatccgg
     1921 tcgaagttgc ccaccacatc cagcccgcct tcagtggtca gctcctgccg tttttccggc
     1981 accaggcaac aatgaggtgg cttgatgaat tcggcataag ccagcatctc gctggtgacg
     2041 gccatttcca gattgacacg ggtctgcagc gtatccatca gaatacgaac atcgcgttcc
     2101 tggatatgac ggcggtcctc cctcaggtgt acggtgatgc catcggcgcc agcctcttcc
     2161 gcatccagcg ccgcctgcac cggatcggga taacgggtgc cgcgcgcctg gcgcaaggtg
     2221 gcgacatgat ccacgttcac acccagcaga attcgattat gcagattcat gatggcctct
     2281 tcattccatg acagcgttga cattgacggc attgagtttg tgcgcaccga cccatgctca
     2341 atccggcgcg atacccgacc aactgcctgg tggaccgacg gctgattttt ctggcaattt
     2401 cagcgtttgg ccagttgctg gaacagttcg cggctgtgca gcggcctgtc gcccagatgt
     2461 ggcgccagcg ccagccggct caactgcttg gccgctgccc gcaccgcgct atcttcatat
     2521 gcctgccggt ggatttcaag caagtgcgcg ccactgaaac aacggcgcag acgggacggt
     2581 tctttcggca gctgcagcaa gcgaacgaaa ccactgtcgg cctcgtacag gtagtggcca
     2641 tcgcgctgca ccggttggcc ttccgtgtcg cggtccagca ccagttcgta gcccagttcc
     2701 agcagcaggt tttgctcaaa cagacgcagt ggaatttccg gcggcatatc ctgcaactgg
     2761 cgcaacgtag cttcgtagtg ctgaaacagc tccggcaggg cttcttcctt gtgcaacagc
     2821 cgcaccagca attcgttcag gtacatgccg ctgaacagtg ccgtgccggt cagcatcacc
     2881 gaggccgccg ccgcttccag cagacgcccg cttttcagat cctgtttgcc gtcaaaggta
     2941 atggcaaggg gggtaaaggg ttgcagcaga cgggctttcg cgccctgcct gccttccttt
     3001 t
//
LOCUS       DASUEG010000884         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1024172, whole genome shotgun sequence.
ACCESSION   DASUEG010000884 DASUEG010000000
VERSION     DASUEG010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024172"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="VFX11_16700"
     CDS             <1..1801
                     /locus_tag="VFX11_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940315.1"
                     /translation="ALDCRAGAILSNRGLLPLVTGWQSGLGETRDVPRISIDRLLKTI
                     LPAEFVPFQAQPDDVAFLQYTSGSTGHPKGVVVSHANLLANFAQILQGFLRDNPVVDW
                     LEELRSVCWLPPFHDMGLIGGILTPIFAGAQVTLMAPMTFLRRPEIWLETISSQRAHI
                     SGGPNFAYEYCLQIVTEGQLQNLDLSRWKVAYNGAEPLQADVLQRFAERFAGCGFNPG
                     AFFPCYGLAEASLFVSGAPGGRGARTLAADQHALQQGRFVAPVPQSPERHQTLVSSGR
                     IAEGTNVRIVQPDTRQPCAEGEVGEIWISGPSVAQGYWAKPALSASTFQARIEQEPHS
                     ACYLRTGDLGFRWQDELYVTGRIKELIIVAGRNLYPQDIEQTVQASHGDFRPRGGTAF
                     GLPGNGREQLVVLQELHRSGNRYHDLALLSANAARAVAARHGVLPAAIVLLPLNALPR
                     TSSGKLQRQEARQLYRDGLLTPLHVWRPGSNLPARGVTQLRPGSAELLHRDWQKELCL
                     EVQLWVAEKLDVEPHHVTLDVTFVDLGIDSVEAVELVKRLQERVGRPIPPIEMLRYPT
                     VKALIENLAAERQQHQLLRLRQECEETFDLGSK"
     gene            2219..3517
                     /locus_tag="VFX11_16705"
     CDS             2219..3517
                     /locus_tag="VFX11_16705"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4940316.1"
                     /translation="MSAFWQGRHRFLRALRVYLGILLSYRRLAKQIRSGTYTRDGITA
                     LQHKVHTENAQRLFQLFQDNGGTWIKAGQCLSGRPDFLPLEYIEALQPLQNNVAPLPF
                     HRIEPVLIRHWGEHWRDRFSQFDEIPVASASIAQVYKARLSDDTQVAIKILIPGIHAL
                     YEQDLVLYRILARVLAALLPQLDVVGTVRVFLDSLHQELDFIQEARNVTTFGQLPHLP
                     RVRSLTLLPELSNADIVTTEWVEGTTLVEYLRHASPDTRYELLSLLQHSYIQQVIRFG
                     IFQGDAHPGNYLIDQDGNLVFVDFGYLGSLTDRERLSYINLILATIDGDARSLQLALR
                     DGGFNCLSDDLFSSRLLELFHTCTRKEGQNIKFAQVEKMVHEVLDCIRQRRANVPAHY
                     LVTGRVFVTLTGLFKLFGVLPERIDIRAILVGSQPVTDTK"
BASE COUNT          777 a         1068 c         1021 g          747 t
ORIGIN      
        1 tgcgctggat tgccgtgcag gcgccatcct gtccaatcgc ggcttgctgc cgctggtaac
       61 cggttggcaa agcgggctgg gtgaaacccg ggatgtgccc cgcatcagta tcgatcgtct
      121 gctgaaaaca attcttcctg cagaatttgt tcctttccag gcccaacccg atgacgtcgc
      181 ttttctgcaa tatacctccg gttccactgg gcaccccaag ggcgtggtgg tatcccatgc
      241 caacctgttg gccaacttcg cccagattct gcaaggcttt ctgcgtgaca atccggtggt
      301 ggactggctc gaagaattgc gctccgtgtg ctggttgcca ccgttccacg atatggggct
      361 gatcggcggc atcctgacac cgatatttgc gggcgcccag gtcacactga tggcgccgat
      421 gaccttcctg cggcgacccg aaatctggct ggaaaccatc tccagccagc gagctcacat
      481 tagcggtgga cccaatttcg cctacgaata ttgcctgcag atagtgactg aagggcaatt
      541 acagaatctg gatctgtcgc gctggaaagt ggcgtacaac ggggccgagc cattacaggc
      601 ggatgttctc cagcgcttcg cagagcgatt tgccggatgc ggctttaacc ccggcgcgtt
      661 ctttccctgc tatggcctgg cggaagcgag cctgttcgtg agtggcgcac cgggcggccg
      721 gggcgcacgc acgcttgcgg ccgatcagca tgcattacag cagggtcgat ttgtggcgcc
      781 agtcccacag tccccggagc ggcatcagac cctggtaagt agcggccgga ttgcggaagg
      841 cacgaatgtc agaatcgtgc agcccgacac ccgccagccc tgcgccgaag gcgaagtggg
      901 tgaaatctgg ataagcggac cgtcggttgc ccagggatat tgggccaagc cggcactgtc
      961 ggcgagcacc tttcaagccc ggatcgaaca ggagccccat tcggcgtgct atttacggac
     1021 tggagatctg gggttccgct ggcaggacga actgtatgtc accggccgca tcaaggagct
     1081 cataatcgta gccggtcgca atctgtatcc ccaggatatc gagcagacag tgcaagcaag
     1141 ccacggggat ttccggcctc ggggcggcac ggcttttggc ttgccgggca acgggcggga
     1201 gcaacttgtc gttctgcagg aactgcatcg aagcggaaac cgttaccatg atcttgccct
     1261 gctctcggcc aatgcagccc gcgcggtggc cgcacggcat ggtgtgttgc cagcggcaat
     1321 tgtgctgctg ccgctcaacg ctctgccgcg aacctccagc ggcaaattgc agcggcagga
     1381 agcacgccaa ctgtatcggg atggcctgct cacaccgttg catgtctggc gccccggcag
     1441 caaccttcct gctcgcggcg tgacgcagtt gcgtcccggc agcgccgaat tactgcatcg
     1501 ggactggcaa aaggagctct gcctggaagt gcagctctgg gtggcggaaa agctggatgt
     1561 ggagccccac catgtgactc tcgatgtaac ctttgtcgat ctggggatcg attcagtgga
     1621 agcggtggag cttgtgaagc gcctgcagga gcgggtcggt cggcccatcc cgccaatcga
     1681 aatgctgcgt taccccaccg taaaggccct gatagagaac ctggcagcag agcgtcaaca
     1741 acatcaacta ctgcgattac ggcaggagtg cgaagagaca ttcgacctgg gttccaaata
     1801 aaacggcgca ggaatacgaa caactggccg ttcagtgcgc ggtgaacgac agggttgcag
     1861 aggacatgct gacagcgaag gagagcccct gcaaaaggcg aacagatatt tcagattggc
     1921 cagacgggaa ttggtgcaga atggcagcaa ggaaatgaat catcggctga ctttttatct
     1981 gagctccatg cggactgaaa aatccggaca gaagtttgaa caaaccaatg aaccaatgaa
     2041 ccaatgaacc aatgaccgat tacaaattct gacaaatatg gcaccacact gctggccaat
     2101 tgcgccacgg tgacaaaatt gtccctttat tcctgcccgt tgagcgggaa taatccagct
     2161 acactcataa ccactgcaac actgtcgtcg tgtgaaaagg attgtcctcg cttccagcgt
     2221 gtctgcattt tggcagggcc gccaccgctt tctgcgcgca ttgcgcgttt atctgggtat
     2281 cctgctgtcc taccgacgtc tggcaaaaca gattcggagc ggcacctata cccgcgacgg
     2341 aatcaccgct ctgcagcaca aagtgcacac tgaaaatgcc caacgcctgt ttcaactgtt
     2401 ccaggacaat ggtggcacct ggatcaaggc aggccagtgc ctgagcgggc gtccggattt
     2461 tctgccactg gaatacatcg aggcgctgca accgctgcag aacaacgtcg cccctctgcc
     2521 cttccaccgt attgaacccg tgctgattcg ccactggggc gagcactggc gcgatcgctt
     2581 cagccagttt gacgaaatac cggttgcctc cgcatcgatt gcacaggtgt ataaagcccg
     2641 tctgtctgat gacacccagg tggccatcaa gattctcatt cccggcattc atgcgctcta
     2701 tgaacaggat ctggtgctct atcgcattct ggcgcgcgtg cttgccgccc tgttgcccca
     2761 gctggacgtg gtgggcaccg tccgtgtttt ccttgattcc ctgcatcagg aactggattt
     2821 cattcaggaa gcccgtaacg tcactacttt tggccagctg ccccatctgc cccgagtgcg
     2881 cagcctgacc ctgctgcccg aactgagcaa tgctgatatc gtgactaccg aatgggtgga
     2941 aggcaccaca ctggtggaat atctgcgcca cgccagcccc gacacccgct acgaattact
     3001 gtcactactg caacacagct atatccagca ggtcatccgc tttggcattt tccagggaga
     3061 tgcccatccc ggaaattacc tgatagatca ggacggcaat ctggtgtttg tggatttcgg
     3121 ttatctgggc agcctgaccg atcgggaacg gctcagctat atcaatctga ttctggccac
     3181 catcgacggc gatgcccgca gcctgcaact ggcgctgcgc gacggcggct tcaactgttt
     3241 gtcagacgat ctgttttcgt cacggctgct ggaactgttc cacacctgca cccgcaagga
     3301 aggccagaac attaaattcg ctcaagtgga aaaaatggta cacgaggtac tcgactgcat
     3361 ccgtcaacgc cgcgccaacg tgccggccca ctatctggtg accggccggg tgtttgtcac
     3421 cctcaccggt ctgttcaagc tgtttggtgt gctacccgaa cgcattgaca tccgcgccat
     3481 cctggtcggc tcccagcccg tcaccgacac caaatgatcc tttaggtgcg aaaaaagcgg
     3541 actgagtgtg caggccagag acgccaacct tgacctgccg caacggtaca accagacccg
     3601 gcgtaaaaat ccg
//
LOCUS       DASUEG010000885         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_542236, whole genome shotgun sequence.
ACCESSION   DASUEG010000885 DASUEG010000000
VERSION     DASUEG010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542236"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFX11_16710"
     CDS             complement(<1..257)
                     /locus_tag="VFX11_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX4940317.1"
                     /translation="MTLTADTRARIESILSSHDVVLFMKGSPQRPQCGFSMKASSIVS
                     GIIPEYAHVDVLQDPEIREGIKEFGQWPTIPQLYVKGELIGG"
     gene            578..1309
                     /locus_tag="VFX11_16715"
     CDS             578..1309
                     /locus_tag="VFX11_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4940318.1"
                     /translation="MSTLIFGGGGMGIVAFSGARSAGAAQEQTRVMVIHDNQFVRQAI
                     QTVLLQAARQFAVQSMDVNTLAQGAWEPESTDVMILGVQRDGAELSFLKTLSLIPSVK
                     VLVMADRADEALVEQSIHLGAKGVFHHCCEPGQLVNAVAAVHRGEFWIERKFISRMLM
                     ESGRRDAKPTGRRLIDDLTPAELKILSAIAKVRGSSNKRVAAHLGLSEHTVRNHLSRI
                     YTKLGLLNRVDLFSFVQRHLPEFAA"
     gene            1713..2378
                     /locus_tag="VFX11_16720"
     CDS             1713..2378
                     /locus_tag="VFX11_16720"
                     /inference="COORDINATES: protein motif:HMM:NF018688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940319.1"
                     /translation="MTRDLNSGLESGSDAVPMGCPPHGRQALIATILLACASSFPLAA
                     ADVPPESWPAAMSLDARESATPAASLFVEQHGLDQHVSGLQLGAGNIGLIEQQGSASS
                     AHLQQAGASQRASVWQDGMANRALVQQAGTNNNAEVEQRGSGNEAEIDQWGSDGFAEI
                     RQVGDNNLASIAQRGDGLTGQIHQIGSNNVYHLFSSGPESAQISVIQVGDSAPPVLIT
                     PGN"
     gene            2483..3409
                     /locus_tag="VFX11_16725"
     CDS             2483..3409
                     /locus_tag="VFX11_16725"
                     /inference="COORDINATES: protein motif:HMM:NF018688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940320.1"
                     /translation="MNQTSSLRASIALVLALGLSSVAVAGNYSTSYQYGNNNQAGTTQ
                     TGNNSSHVVQSGSANQAATTQVGSSWGYAQQYGNANNASLNQSGQNGGYLYQSGEGNY
                     AAASQNGYNHAQSVQQGTSNGSTTNQAGQNSSIGYQQGSYNGSYANQQGQNTGSVWVR
                     GDYNSTSIYQSGNSSPSQQQSVNIQIGGETVSNNNWANASQVGEGNQSTALINGNSNG
                     NYTGQYGADNVAKNRTTGTGNWTHANQNGERNLVAGVDEEEFMTVEGYGNRAYLYQSG
                     SDNEVHASQSGTYNNMYVNQYGSANVATVIQN"
BASE COUNT          724 a          962 c         1073 g          666 t
ORIGIN      
        1 ccgccgatca attcgccttt cacatagagc tgggggatgg tcggccactg gccgaattcc
       61 ttgatgccct cgcggatctc cggatcctga agcacatcga cgtgcgcgta ctcgggaatg
      121 attccggaga cgatgctcga ggctttcatc gagaagccgc actgggggcg ctgcgggctg
      181 cccttcatga acagcacgac atcgtgcgag gacaggatgg attcgatgcg ggcgcgggtg
      241 tcggcggtca aggtcatgga gggaatcacg caattggtta gcccggcatt gtagcggcct
      301 gttctcgctg gcccatgaag gcaggtgtct ggccgggtca gggtggtggg ttgagtaaaa
      361 ggggaatgcc gtaccgccca ggtgcgactg tgaatccttg ttaaggagca gggcggaatg
      421 gggcttttct ctcacagggt gataaaaatg tgaggcactc cgcattttag gggggcggcc
      481 ggcgcgatca cccgtcaaaa cgggatcgcg acgcggtggg aacgggcggg acgccctgag
      541 ggtccaccac ggctgcttga tgcgggcaaa ggacgaggtg agtacgttga tttttggggg
      601 tggcggaatg ggaatcgtcg ctttttctgg ggctcgctcg gcgggcgcgg cccaggaaca
      661 gactcgggtg atggtgatcc acgacaacca gtttgtgcgt caggccattc aaactgtcct
      721 gctgcaagcg gcgcggcagt tcgccgtcca gtcgatggat gtaaacaccc tggcacaggg
      781 ggcgtgggag cccgagtcga cggatgtgat gattctgggc gtgcagcggg atggagccga
      841 actgtcgttc ctgaagacgc tgtccctgat tccgtcggtc aaagtcctcg tgatggccga
      901 tcgtgccgac gaggcgttgg tggagcagtc catccatctg ggggccaaag gcgtcttcca
      961 ccattgttgc gagcccggcc aattggtaaa cgccgtggcg gctgtgcatc gtggggagtt
     1021 ttggattgag cgaaagttca tcagccgaat gctgatggag tccggcaggc gcgatgccaa
     1081 accgacaggc cgtcgattaa tcgatgatct gacaccggcc gagctcaaaa tcctctcggc
     1141 catcgccaag gttcggggca gttccaacaa gcgtgttgct gcacatcttg ggctgagcga
     1201 gcacacggta cgcaatcacc tctcgcgcat ctataccaaa ctcggcttgc tcaatcgcgt
     1261 cgacctgttc tcgttcgtcc aacgccatct ccccgaattc gctgcttgat tgaccttgag
     1321 cggtgacgcc gctcgggcgg tcttcccctc attaaggcat cgcgccaggc gccgtgggga
     1381 ggcatcatcc ctaggctgct gcttgcgcag ctcagttctt ctcgccgatc tgtaaaccga
     1441 ctattccatt cgagcgtatc gactggcgta cgggaaaaat gcgtcagcgt cttcccgcca
     1501 gcagcatttc tccaagggtc tgacttgcgg cgtccgaatc gcggcatgtg ttgggattcc
     1561 atgccagaag tagtagcgct gcaccacgtg gcaggtacat ctgtaccagc gaaggttgag
     1621 cgtttctgct gtggtgtcat gggacaactg gaacgcgcct gatgtcgggc gaactggtct
     1681 gatgagcgct agaaacgaga tgtcgaggcg ccatgacgcg ggatctgaac agtgggctag
     1741 aaagcggcag tgacgcggtg ccgatgggct gcccgccaca cggtcggcaa gcacttattg
     1801 ccaccattct gctggcctgc gcttcgtctt ttccgctggc ggcggcagac gtcccgcctg
     1861 aatcctggcc tgcagccatg agtctggatg cgagggaaag cgcaactccc gcagcgtcgt
     1921 tgtttgttga acaacatggt ctggatcagc atgtcagtgg cttgcagctt ggcgccggaa
     1981 acatcggctt gattgagcag cagggcagtg cgtcctctgc gcatttgcag caagcgggtg
     2041 cttcgcagcg tgcctcggtt tggcaggacg gaatggccaa ccgggcgctc gttcaacagg
     2101 caggcactaa caacaatgca gaggtcgagc agcgcggcag tggcaacgag gccgagatcg
     2161 atcagtgggg gagtgacggg ttcgccgaga tccgccaagt gggcgacaac aacctggcga
     2221 gcattgccca gcgcggcgat gggctcactg gccagatcca tcagatcggc tccaacaacg
     2281 tgtaccacct tttttcttct gggccagagt cagcccagat cagcgttatc caagtcggtg
     2341 acagcgcccc tccggtgctc atcactcccg gtaactagcc ggaggtgtgg cggagacaac
     2401 ggcttaccgt tttggtgacg ggcgagaaac gccctgtggc aagtgtgtgc atatctcaat
     2461 catcctcaag gagcttcatc aaatgaatca gacatcatcg cttcgtgcca gcatcgccct
     2521 cgtgcttgct ctcggtcttt cctcggtggc ggttgccggc aactactcga cgtcgtatca
     2581 gtacggcaac aacaatcagg ccggcaccac ccagacgggt aacaacagca gccacgtcgt
     2641 ccagagcggt agcgccaatc aggctgctac cacccaggtg ggctccagct ggggctacgc
     2701 ccagcaatac ggtaacgcga ataatgccag tctgaaccag agcggccaga atggcggtta
     2761 tctgtatcag agcggcgagg gcaactacgc tgccgcctca cagaatggct acaaccatgc
     2821 gcagtcggtg cagcaaggca ccagcaacgg ttcaaccact aaccaggcag gacagaacag
     2881 cagcattggt taccagcagg gctcctacaa cggcagctat gccaatcagc agggtcagaa
     2941 cacggggtcg gtgtgggttc gtggtgatta caactccacc agcatttacc agagcggcaa
     3001 cagcagccca agccagcagc agtcggtgaa tatccagatt ggtggcgaga cggtctccaa
     3061 caacaactgg gccaacgctt ctcaggtggg tgaaggcaac cagtccaccg cgctgatcaa
     3121 tggcaacagc aacggcaact acaccggtca gtatggcgcg gacaacgtcg ccaagaaccg
     3181 caccacgggc accggcaact ggactcatgc caaccagaat ggtgagcgca acctcgtggc
     3241 cggtgtcgat gaagaagagt tcatgaccgt tgagggctat ggcaaccgcg cctatctgta
     3301 ccagagtggt tcggacaacg aagtgcacgc cagccagtcg ggtacttaca acaacatgta
     3361 tgtgaaccag tacggctcgg cgaacgtcgc caccgttatt cagaactgag tgaacgtctc
     3421 aagga
//
LOCUS       DASUEG010000886         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_567301, whole genome shotgun sequence.
ACCESSION   DASUEG010000886 DASUEG010000000
VERSION     DASUEG010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567301"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VFX11_16730"
     CDS             complement(<1..914)
                     /locus_tag="VFX11_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940321.1"
                     /translation="MSSDQRKRVILATLAGATSLLVLFLLHGEGTSTGVLMLVVVSGV
                     LWSLALRLAGKLQGVPHPAHAPQRIRPFGAPRLAAAADPPPDPLGHYFSHLLGSLEHT
                     WLVFRKHYRQTEKSLHNVKVSLDKAMELSRDTGLLAANAMMASASCGEIGRGFVTVSQ
                     DLIDISERSGDDLLQLRQLVSSLASELERLGTLVEHPLEFWVVSSAGLPVRELINLRS
                     GIAISQHELRALAERYRRSPRQDVRWLQLGDAIKRLLNELINTLFQLELHLDDVLSDM
                     RLLKLAEGPGSWQQIVEIKDRLHAAREPG"
     gene            complement(921..2021)
                     /locus_tag="VFX11_16735"
     CDS             complement(921..2021)
                     /locus_tag="VFX11_16735"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX4940322.1"
                     /translation="MLSNGANPTGKIRVLVVDDSAVIRQLLSQIINAESDMEVVGTAS
                     DPLIAREKIKQLAPDVLTLDIEMPRMDGITFLRNLMRLRPMPVIMISTLTQVGADVTL
                     EALNIGAFDFLPKPRENLGKGLLEYGDDITRKIRAAARANVRQLERTSEQTRAAPQKP
                     VTRVADYSPRPDALIAIGASTGGTEAIKEVLLRLPENCPPVVITQHIPAVFSTSYALR
                     MDNTCAMHVHEASANQKILPGNVYIAPGDFHLLVRRGSDGWVTALSSAPKVNHHRPSA
                     DVMFDSIVDLALTGAVGVLLTGMGSDGAKGLLRLKQIGCHTIIQDQESSVVWGMPGAA
                     FQIDAHCEMKPLDGIATRLLALTNNRTRSREA"
BASE COUNT          423 a          651 c          686 g          412 t
ORIGIN      
        1 cctggctcgc gagccgcatg caatctgtct tttatttcaa caatttgttg ccaggaaccg
       61 gggccttcgg ccagtttcag caggcgcata tcagataaca catcatcaag gtgcaactca
      121 agctgaaaca gtgtgttaat cagttcgttg agcaggcgct tgatggcgtc gcccagttgc
      181 agccagcgca catcctggcg gggtgaacgg cgatagcgct cggccagcgc gcgcagttca
      241 tgctggctga tggcgatgcc ggagcgaaga ttgatcagct cacgcacggg caggccggca
      301 ctggacacaa cccagaactc cagcggatgt tccaccagcg tgcccaggcg ttccagttcg
      361 ctggccaggc tgctcaccag ttgccgcagc tggagtaaat catccccgga acgttcgctg
      421 atgtcgatga gatcctggga caccgtgacg aagccgcgcc cgatctcgcc acacgaagcg
      481 gacgccatca tggcattggc ggccagcagg ccggtatcac ggctcagttc catcgccttg
      541 tcgagagaca ctttcacatt gtgcagggat ttttcggttt gacggtagtg tttgcgaaac
      601 accagccagg tgtgttcgag actgcccaac aggtggctga agtagtgacc cagcggatcg
      661 ggcggcggat cggcagcagc ggccagccgg ggtgcgccga acgggcgtat gcgctgcggc
      721 gcgtgcgccg gatgcggcac accttgcagc ttgcccgcca ggcgcagggc aaggctccag
      781 agtacgccgg acacaaccac cagcatcagc acaccggtag atgtcccctc cccgtgcaac
      841 aggaacagca ccagcagact ggtggcacca gccagagtgg caagaatgac gcgcttgcgt
      901 tggtcgctgg acatggtccg tcaggcttcc cgggagcggg tgcggttgtt ggtaagggcc
      961 agcaggcggg tagcgatgcc atccaggggc ttcatttcac agtgcgcatc aatctggaaa
     1021 gccgcgcccg gcatgcccca taccaccgac gactcctggt cttggataat agtgtgacag
     1081 ccgatctgct tgagacgcaa caagcccttc gcaccgtcgg agcccatgcc ggtcaagagc
     1141 acgccaacgg cgccggtcaa tgccagatcg acgatggaat cgaacatgac atccgccgac
     1201 ggccggtggt gattgacctt gggcgcgctg ctcagggccg tgacccagcc gtcgctgccg
     1261 cgacgcacca gcagatggaa atccccgggg gcaatgtaga cattgccggg caggattttc
     1321 tggttggcac tggcctcatg cacatgcatg gcgcaggtgt tgtccatgcg tagcgcgtag
     1381 gacgtactga acacggcggg aatgtgttgg gtgatgacca ctggcgggca attctccggt
     1441 aaccgcagca gcacttcctt gatcgcctcg gtgccgccgg tggatgcgcc aatggcaatg
     1501 agtgcgtcgg gccggggcga gtaatccgcc acccgggtga ccggcttttg cggcgcggcc
     1561 cgcgtctgct cgctggtgcg ttccagctgg cgcacgttgg cccgggcggc agcacggatt
     1621 ttgcgggtga tgtcgtcacc atactccagc agccccttgc ccaggttttc gcggggcttg
     1681 ggcaggaaat cgaaggcgcc gatattcagc gcttccagcg tgacgtcggc gcccacctgg
     1741 gtaagggtgg aaatcatgat caccggcatg ggccgcaggc gcatcaggtt gcgcaggaaa
     1801 gtgatgccgt ccatgcgggg catctcgatg tcgagagtca gcacgtcagg tgccagctgc
     1861 ttgatctttt cccgggcgat gagcggatca gacgcggtac ccaccacttc catgtcggac
     1921 tcggcgttga tgatctggct gagcagctgc cggatcaccg cagaatcatc taccaccagc
     1981 acccggatct tgcctgtcgg gttcgcacca ttgctcaaca tgccggacac cattcagaac
     2041 agctcgatat cgccctgcgg cggccgggta ttgatggtac gggcgtaact gcgctcgcgc
     2101 tcgtacacga catcattgcg agtggatttc attttgcgca ctttcactga tcccgtatcc
     2161 ggaaaataaa gg
//
LOCUS       DASUEG010000887         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_740617, whole genome shotgun sequence.
ACCESSION   DASUEG010000887 DASUEG010000000
VERSION     DASUEG010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740617"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFX11_16740"
     CDS             complement(<1..592)
                     /locus_tag="VFX11_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4940323.1"
                     /translation="MARTTPLKRYRNIGISAHIDAGKTTTTERVLFYTGKSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWSGMDKQFEEHQINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYRVPRIAFVNKMDRTGANFLRAVEQMRT
                     RLGANPVPVVLPIGAEENFEGVIDLVKMKAIYWDEAS"
     gene            complement(611..1081)
                     /gene="rpsG"
                     /locus_tag="VFX11_16745"
     CDS             complement(611..1081)
                     /gene="rpsG"
                     /locus_tag="VFX11_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX4940324.1"
                     /translation="MPRRRVVAAREILPDPKFGSQTIAKFINHVMERGKKSIAESVVY
                     GALERVAEKTKGDPVEFFENTLEKVRPMVEVKARRVGGATYQVPMEVRPSRRTALAMR
                     WLVDSARKRSEKSMALRLAGELLDAAEGKGAAIKKREDVHRMAEANKAFSHYRF"
     gene            complement(1169..1543)
                     /gene="rpsL"
                     /locus_tag="VFX11_16750"
     CDS             complement(1169..1543)
                     /gene="rpsL"
                     /locus_tag="VFX11_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX4940325.1"
                     /translation="MATTNQLIRKGRTALVEKSGVPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTSGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DCAGVKDRNQSRSKYGTKRPKK"
     gene            complement(1674..>2554)
                     /locus_tag="VFX11_16755"
     CDS             complement(1674..>2554)
                     /locus_tag="VFX11_16755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004932377.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4940326.1"
                     /translation="ALQDGAMVGVGDVIGRIPMESSKTRDITGGLPRVADLFEARKPK
                     ESAILAEKSGVVSFGKETKGKHRLIITPDDGSEIYEELIPKWRQINVFEGERVSRGET
                     ISDGPQNPHDILRLKGETALATYIVNEVQDVYRLQGVKINDKHIEVIIRQMLRKVEIV
                     DSGDSSLIKGEQVEYGRVMEENEALKGADRFPAKFDRILMGITKASLATESFISAASF
                     QETTRVLTEAAVTGKEDDLRGLKENVVVGRLIPAGTGFAYHAERRRARQAERAVTAPT
                     ASDVEQALSDALNQGE"
BASE COUNT          541 a          773 c          693 g          547 t
ORIGIN      
        1 gagatgcttc atcccagtag atagccttca ttttgaccag atcgatcacg ccttcgaagt
       61 tttcttcggc accgatcggc aacacaaccg gaacaggatt ggcacccaag cgggtcctca
      121 tctgctcgac agcgcgcagg aagttggcac cagtacggtc catcttgttg acaaaggcaa
      181 tgcgcggcac gcgatacttg ttagcctgac gccagaccgt ttccgactgc ggctgcacgc
      241 cacccacggc acagtaaacc atgcaggcgc catcaagcac gcgcatggaa cgctccacct
      301 caatcgtgaa gtcaacgtgg cccggggtat caatgatgtt gatctgatgc tcttcgaact
      361 gcttgtccat gccggaccag aaacaagtgg ttgcagcaga ggtaatcgtg atgcctcgct
      421 cctgctcctg ctccatccag tccatggtgg ccgcgccgtc gtgcacctca ccaatcttgt
      481 gcgacttgcc cgtgtagaac aggacacgct cggtagtggt ggtcttgcca gcatcaatgt
      541 gcgcactgat gccgatgttg cgatagcgct tgagtggggt ggttcttgcc atgattcgta
      601 tctccggcaa ttagaagcga tagtgcgaga aggccttgtt ggcctccgcc atgcggtgca
      661 cgtcttcacg cttcttgatg gcagcgccct tgccttctgc ggcatccagg agctcaccag
      721 ccagacgcag cgccatgctc ttttcagagc gcttgcgggc ggagtccacc agccagcgca
      781 tggccagggc agtacgacgg gagggacgta cttccatggg cacctggtaa gtggcaccgc
      841 caacacggcg agccttcact tcaaccatcg gacgaacttt ctcgagggta ttctcgaaaa
      901 actcaaccgg atcacccttt gtcttctcgg caacacgctc aagcgcgcca taaaccacgg
      961 actcagcgat ggacttcttg ccgcgctcca tcacatggtt gatgaacttg gcgatagtct
     1021 ggctaccgaa cttgggatcc ggaagaattt cacgggctgc tactacacgt cttcttggca
     1081 ttttctcatt aacctttcat tacgcttcag ggttgtccgg gacttaaagt tggaaactga
     1141 aatgcccggg ccttactgcg ccacaagatt acttcttggg acgcttggtg ccgtacttgg
     1201 aacggctctg gttgcgatcc ttgacgccgg cgcaatccag ggaaccacgc acggtgtggt
     1261 aacgcacacc tggcagatcc ttgacacgac caccacgaat caggacaaca gagtgctcct
     1321 gcaggttgtg gccctcgccc ccgatgtagg agctgacttc aaagcccgag gtcagacgaa
     1381 cacggcaaac cttgcggagc gcggagttcg gcttcttcgg agtagtggta tagacacgcg
     1441 tgcagacgcc acggcgctgc gggctgccct tgagcgcggg cacgccagac ttctcgacga
     1501 gcgccgtgcg acccttgcgg atcagctggt tggtggttgc catggtggca atattctccc
     1561 gtagtaataa aaaaccccca aaaaagactt agcctttttg agggttgcga attttatgga
     1621 ttgctgcccg cgacgtcaag atggtcccgg gcagcaatcg acacacggca gggttattcg
     1681 ccctgattca gtgcatccga caaggcctgc tcgacatcac tggccgtagg agcagtaacg
     1741 gcacgctcag cctgacgggc gcggcgacgc tcggcatgat aagcaaagcc ggtaccagcc
     1801 ggaatgagac ggccaaccac cacattctcc ttcaggccac gcaggtcatc ttccttgccg
     1861 gtcaccgctg cttccgtcag gactcgggtg gtctcctgga aggaggctgc cgagatgaag
     1921 gactccgtgg ccaacgatgc cttggtgata cccatcagga tacggtcaaa cttggccggg
     1981 aagcggtcag cccccttaag tgcctcgttc tcttccatga cacgaccgta ttcaacctgc
     2041 tcacccttga tcagagagga gtcaccgctg tcaacgatct ccaccttgcg caacatctgg
     2101 cgaatgatga cttcgatgtg cttgtcgttg atcttcacgc cctgcagacg gtaaacgtcc
     2161 tgtacctcat tgacgatata cgtcgccaga gcagtttcac ccttgaggcg caggatgtcg
     2221 tgcgggttct gcggaccatc cgaaatggtt tcgccgcggg acacacgctc cccttcgaac
     2281 acgttgatct gacgccactt cggaatcagc tcttcataaa tctcggaccc gtcatccggc
     2341 gtaatgatca gacgatgctt gcccttcgtc tccttgccga aagacaccac gccggacttt
     2401 tcagccagga tcgcggattc cttgggcttg cgggcttcaa agaggtcagc cacgcgcggc
     2461 agaccaccgg tgatgtcacg ggtcttagac gactccatcg gaatacgacc gataacgtca
     2521 cccacaccca ccatggcacc atcctgcagg gcag
//
LOCUS       DASUEG010000888         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2125469, whole genome shotgun sequence.
ACCESSION   DASUEG010000888 DASUEG010000000
VERSION     DASUEG010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125469"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFX11_16760"
     CDS             complement(<1..260)
                     /locus_tag="VFX11_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4940327.1"
                     /translation="MELLSGAEMIVRSLKDEGVETIFGYPGGAVLHIYDALFQQKGIT
                     HVLVRHEQAAAHAADGFARVSGKPGVVLATSGPGATNTVTGIA"
     gene            780..1244
                     /locus_tag="VFX11_16765"
     CDS             780..1244
                     /locus_tag="VFX11_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4940328.1"
                     /translation="MKTYRSAVLAAALVSFCFVSAAEAAGKYYKWTDSAGVVHYGENP
                     PDPSKAQKVSVSTGVPSGQPATEEDLEKKQAKLMGDAEAEKEKQDEKTTTEENAKIVK
                     ENCDIYKQNLSALKNSARIREKDAKGEYRYLTDEEKAERTKSAETYIQENCK"
     gene            complement(1369..>3154)
                     /locus_tag="VFX11_16770"
     CDS             complement(1369..>3154)
                     /locus_tag="VFX11_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4940329.1"
                     /translation="NELAKEEGSVILFIDELHTMVGAGKGEGAMDAGNMLKPALARGE
                     LHCVGATTLDEYRQYIEKDAALERRFQKVLVDQPTVEDTIAILRGLKERYEVHHGVDI
                     TDSAIVEAAKLSHRYITDRQLPDKAIDLIDEAASRIRMEMDSKPEELDRLERRLIQLK
                     MEREALKKEEDEASRKRLEKLNEDIGRIEREYADLEEIWTAEKAALQGSQQIRAELEQ
                     ARIDMEHARRAGDLARMSELQYGRIPDLEKRLDMAAQAEMMEMKLLRNKVTEEEIAEV
                     VSKWTGIPVSKMLEGERDKLLRMEEALHQRVIGQHEAIVAVSNAIRRSRAGLSDPNRP
                     NGSFLFLGPTGVGKTELCKALANFLFDTDDAMVRIDMSEFMEKHSVARLIGAPPGYVG
                     YEEGGYLTELVRRKPYSVILLDEVEKAHPDVFNILLQVLEDGRLTDGQGRTVDFKNTV
                     IVMTSNMGSEIIQTMTGEDEYDRMKAAVMDIVATKFRPELINRIDEVVVFHPLAHSQI
                     RGIATIQLANLRKRLAERDLSLELSDAVMDKLVEAGFDPVYGARPLKRAIQREIENPL
                     AQDILSGRFLPRDTIRGELREGRIVFGK"
BASE COUNT          626 a          915 c          924 g          689 t
ORIGIN      
        1 gctataccgg tcaccgtatt ggtggcgccg ggaccggacg tggccagcac cacaccgggt
       61 ttaccgctga cccgggcaaa accgtccgcc gcatgggcag ccgcctgctc gtgacggacc
      121 aggacgtgag taatgccttt ctgctggaac agtgcgtcat agatatgaag tactgccccg
      181 ccgggataac caaagatggt ttcaacccct tcgtccttga gggagcggac gatcatttct
      241 gcgcctgata agagttccac gatctcacac ctcatcttga acaacgccat ccggaggcca
      301 agccggggga tgctcaagca tttgcttctg cgttaatggt tcagtgaatt cagttccctg
      361 catgcgcggc aatggttgcg tacagcctgg gactgtgggt ttaagctagt gctggcagaa
      421 aaaccaaggg ttggggcttg ttccacctca tacgctttgg gcatatgatt cttccagcga
      481 gatcgacggg gtaaagcaga tggtgactgc gattaaatgg cgaaattctc gctctagcgc
      541 aggcggtatg gggtttgtcc cggcggcctg cacccacctc agtgagccgc ttatgttgac
      601 acggttaagg attttgtcaa tagacgccaa ttccctgcaa aagcgggtga atctgtccgt
      661 ttaggtcgcc gggcaggcag atttccgacc ctttcacagt ttgttcctga ttttctacgc
      721 gctggacagt taacccgcta tattgaatag gagaattttc catggagctt ggcatagcaa
      781 tgaaaaccta tcgttccgcg gtcttggcgg ctgccctggt gtccttctgt ttcgtttccg
      841 cggccgaggc tgcgggcaag tattacaagt ggacagacag cgcgggggtc gtccattacg
      901 gcgaaaaccc gcccgacccc agcaaggcgc agaaggtgag cgtgagtacc ggagtgcctt
      961 ccggccagcc ggcgacggaa gaggatctgg agaaaaagca ggccaagctg atgggcgatg
     1021 cggaagcgga aaaggaaaag caggacgaaa agacgacaac cgaagaaaat gccaaaatcg
     1081 tcaaggaaaa ctgtgacatc tataagcaaa acctgagcgc cctgaaaaac agcgcccgca
     1141 tccgcgaaaa ggatgccaag ggtgagtatc gctacctgac cgatgaagaa aaggccgagc
     1201 gcaccaagtc agccgagacc tatatccagg aaaactgcaa ataaggcagt tttcctggcc
     1261 gctcaccttg tggggacggc gcgcataaca aaacccccgg cctgaaaaac cgggggtttt
     1321 gttttgtcag gaaaaacctg accgcagaac acctgatcca gacccggctt acttgccgaa
     1381 tacgatcctg ccctcccgca gttcgccccg gatggtatcc cgcgggagga agcggccgga
     1441 caggatgtcc tgggccagcg ggttctcgat ctcccgctgg atggcgcgct tgagcggacg
     1501 ggcaccgtag accgggtcga aaccggcttc caccagtttg tccatcacgg catccgacag
     1561 ctccagactc agatcccgct ctgccagccg cttgcgcagg ttggccagct ggatggtggc
     1621 gataccgcgg atctggctgt gggccagcgg gtggaacacc accacctcgt cgatccggtt
     1681 gatcagctcg ggacggaact tggtggccac gatgtccatc acggcggcct tcatgcggtc
     1741 gtattcgtcc tcgccggtca tggtctggat gatttcagag cccatattgg aggtcatgac
     1801 gattaccgtg ttcttgaaat ccactgtgcg cccctgaccg tcggttagac ggccatcttc
     1861 cagcacctgc agcaggatgt tgaacacatc cggatgtgcc ttctccacct cgtccagcag
     1921 gatcaccgag tagggtttgc ggcgcaccag ctcagtcagg tagccacctt cctcataccc
     1981 cacatagccc ggcggagcgc cgatcagacg ggccacggaa tgcttttcca tgaactccga
     2041 catgtcgatg cgaaccatgg cgtcgtcggt gtcgaacagg aagtttgcca gcgccttgca
     2101 cagttcggtc ttgcccacgc cggtggggcc caggaacagg aacgagccgt tggggcggtt
     2161 ggggtccgac aggccggcgc gggaacgacg gatcgcattc gacacggcga caatcgcttc
     2221 atgctgcccg atcacgcgct ggtgcagagc ctcttccatc cgcaacagtt tgtcgcgctc
     2281 gccttccagc attttggaca cgggaatacc ggtccacttg gaaaccactt cggcgatttc
     2341 ttcctcggtg accttgttgc gcagcagttt catttccatc atctcggcct gcgcggccat
     2401 gtcgagacgt ttttccaggt cgggaatgcg gccgtactgc aattcagaca tgcgcgccag
     2461 atcgccggca cggcgggcgt gttccatgtc gatgcgggcc tgctccagtt ccgcccggat
     2521 ctgctggctg ccctgcagtg cagctttttc ggcggtccag atttcttcca ggtcggcgta
     2581 ttcgcgttcg atgcgtccga tgtcctcgtt gagtttttcc agccgtttgc gggaggcttc
     2641 atcttcctct tttttcagcg cctcgcgttc cattttcagc tggatgagac ggcgttcgag
     2701 gcggtcgagt tcttccggct tggaatccat ttccatgcgg atgcggctgg cggcttcgtc
     2761 gatcaggtcg atggctttat ccggcagctg gcggtcggta atgtagcggt gcgacagttt
     2821 tgccgcttcc acgatggcgg aatcagtgat atcgacaccg tggtgcactt cataccgttc
     2881 tttcagaccg cgcaggatgg cgatggtgtc ttccacggtg ggctggtcca ccagcacttt
     2941 ctggaaacgg cgttccagcg cggcatcctt ttcgatgtat tggcgatatt catccagcgt
     3001 agtggcgccg acgcagtgca gttcgccgcg agccagcgcc ggtttcagca tgttgccggc
     3061 atccatcgcg ccttcgccct tgccagcgcc gaccatggtg tgcagctcgt cgatgaagag
     3121 gatcacactg ccctcttcct tcgccagttc gttc
//
LOCUS       DASUEG010000889         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1207640, whole genome shotgun sequence.
ACCESSION   DASUEG010000889 DASUEG010000000
VERSION     DASUEG010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207640"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..1093)
                     /locus_tag="VFX11_16775"
     CDS             complement(371..1093)
                     /locus_tag="VFX11_16775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940330.1"
                     /translation="MRGKEYTPGDFESTKHDNLRPQFYEGMNTIKTLGYETEDFIHHF
                     PCFTGHLTLARFISFYESYRQTLGLAGHIAEIGMFRGAVSFFFAKLIKHFEPYAMTQV
                     HGFDWFQGNQPTEADKNITPGGYTESYERVKKLVECQGLDNILKVHKLDVTQELEGFF
                     EKYSHLQFKIVFLDAGQHEIVSAVLPYFWERLNKGGMMILDQYNFDVAPGETRAVREF
                     FGNVQVRTFPNGWMPTAYVVKE"
     gene            complement(1104..1766)
                     /locus_tag="VFX11_16780"
     CDS             complement(1104..1766)
                     /locus_tag="VFX11_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX4940331.1"
                     /translation="MVDIVMFGSGQFAEVAVAYLERDTKHNIVAFTLDQEYIEEPTFQ
                     GKPVVPFETLEEHYPPDRVELFLPLSYKKMNRYREEHYLNAKARGYRFITYVSPHARI
                     HSHTRIGENCMILDDNVIQPYVEIGNNCILWSGNHIGHHTKIRDNVFIASHAVVSGAV
                     EIGNNCFLGVNCTIRDNIKIAPYCLIGAGAVILKSTREKQVFIGPKSEAADISTDDLK
                     NI"
     gene            complement(1807..>2111)
                     /locus_tag="VFX11_16785"
     CDS             complement(1807..>2111)
                     /locus_tag="VFX11_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016959526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4940332.1"
                     /translation="DITDVISAYLQRGDLNVELLGRGTAWLDTGTHDALLDAGNFIKL
                     IEDRQSLKICCPEEISYLMGYIDDNQLERLIVPLQKSGYGQYLQRILDENRRFSAN"
BASE COUNT          432 a          532 c          583 g          564 t
ORIGIN      
        1 tggcggagat aagggtgttt aatgagcctt caataggtga tccacttgag tcgtggtggt
       61 aagcgagtat ccagttcttg aactcacttg cgtagctgtt gtttggcgca gtagccaaaa
      121 gggctgatat tgacagtaag catagtgcaa ccgtgtgttt catggcgtat gtccttgtgc
      181 tgatatgatt tagttatttg tcactcgcat atggtctgaa tggcggtcta atcatagacg
      241 tatgccacaa tctgctgatt gaattggtat tattcacgcc gcgtttctat tggatgaata
      301 gtgtgagctc tatgtctatt tcctggttct cgataatcat gtgattcttg tcgatcttat
      361 ggcgagaccg ttattccttc acgacgtagg ccgtcggcat ccatccattc ggaaaagtcc
      421 tcacctgaac attgccaaaa aactcccgca ccgctcgcgt ctcgcccggc gcaacatcga
      481 agttgtactg gtccagtatc atcatgccac ccttgttgag gcgctcccag aagtagggca
      541 gcactgcgct gacgatttca tgctggcccg catccaggaa cacgatcttg aattgcaggt
      601 ggctgtattt ttcaaaaaat ccctcaagct cctgggtgac gtccagcttg tgcaccttca
      661 ggatgttgtc cagaccctgg cattccacca gttttttaac ccgctcatag gattcggtgt
      721 agccgccggg cgtaatgttt ttgtcggcct cggtgggctg gttgccctgg aaccagtcga
      781 agccatgcac ctgggtcatg gcatagggct caaagtgctt tatcagcttg gcgaagaaga
      841 atgagactgc gccgcgaaac atgccgatct cggcgatatg gcccgccagc cccagggtct
      901 ggcggtagct ttcatagaag gaaatgaagc gggcaagggt gaggtggccg gtaaagcacg
      961 gaaaatggtg aatgaaatct tcggtttcat aacccagcgt cttgatggtg ttcatgcctt
     1021 catagaactg ggggcgcagg ttgtcgtgct tggtgctttc gaaatcgccg ggtgtatatt
     1081 ccttgccacg catggttttt tcctcagata tttttcaggt cgtcggtgct gatgtcggcg
     1141 gcctctgatt tcggtccgat gaacacctgt ttttccctgg tggacttgag aatgactgcg
     1201 cctgcgccaa tcagacagta aggtgcaatc ttgatgttgt cgcgaatcgt gcagttcacg
     1261 ccaagaaagc agttgttgcc gatctccacg gcgccagaca ccaccgcgtg cgatgcaata
     1321 aatacattgt cgcggatttt ggtgtgatga ccgatgtgat tgcccgacca caggatgcag
     1381 ttgttgccaa tctccacgta gggctgaatg acgttgtcgt ccaggatcat gcagttctca
     1441 ccaatcctgg tgtggctgtg aatgcgggcg tggggcgaca cataggtgat gaaccggtag
     1501 ccgcgcgcct tggcgttgag gtagtgctct tcgcggtaac ggttcatttt tttgtagctg
     1561 agcggcagga acagttcaac gcgatccggc gggtagtgtt cctccagcgt ttcgaatggc
     1621 actaccggct tgccctggaa cgtaggctct tcgatatatt cctggtcgag ggtaaatgcc
     1681 acgatattgt gcttcgtgtc gcgctccaga taggcgacgg ccacttccgc aaactggcca
     1741 ctgccgaaca ttacgatgtc taccatttgg ctccccttgt gtccgatggc attccggttg
     1801 cttcccctaa ttcgctgaaa agcggcggtt ttcgtccagt atgcgttgca ggtattgccc
     1861 gtagccgctc ttttgcagcg gcacgatcag gcgttccagc tggttgtcat cgatgtatcc
     1921 catcagatag ctgatttcct ccgggcagca gattttcaac gactgccggt cctcgatcag
     1981 cttgatgaaa ttgccggcat ccagcagcgc atcgtgagta ccggtatcga gccaggcggt
     2041 gccgcgcccg agcaattcca cattcagatc gccacgctgc agataggcgc tgatgacatc
     2101 ggtgatgtcc a
//
LOCUS       DASUEG010000890         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_921889, whole genome shotgun sequence.
ACCESSION   DASUEG010000890 DASUEG010000000
VERSION     DASUEG010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921889"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VFX11_16790"
     CDS             complement(<1..1784)
                     /locus_tag="VFX11_16790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940333.1"
                     /translation="MNQTLPLIIIADNQDANIAYYSELVQASGARALATAEGGNVLNL
                     VASHQPALLILDSALNDPDSYQVLNLLKSDSRTLHIPVLFITGNLSERKMCLHQELFR
                     VVDVLAKPVAERVLQHRVKIYLQLQQYRNQIMAMGSENKKSLVENREEGVLALDRQGQ
                     ILYANAAAERLLKAPVLDLAGNYLETILEEACSGVRSRWKDHPIAKVTRTEQILQVDK
                     AMLWRADGESISVKFAAIPVNTLPEIGLLFAFRQLKDTRESKDKIAKLSHVDHLTNLP
                     LRAPIEEHIDRCVLKAGISGFYFAVLYVDLDHFRYINESLGHDRGDQLIREVAQRINS
                     LVRRDDMVGRMEGDEFVVVLGHIDLPDNAGIVAQKIIDQIRETFLIDGHQVFTGCSIG
                     VSVYPTCGDDARTLLKNAESALARAKAIGRNNYQYYTAEMNKQRVEQMQIEYELHQAV
                     EQKQWRVQFQPVAAVENDQLVACEIKLSWVNPARGELMLDEFLPAAEESGLAPVIFRW
                     FWQHALERVSTMNARQRENVKLILPVSPSLLMQEGGVEWVTGVVRAQGIRTEQIYVGL
                     PESSLAIRHSQQSEVLNQLRKNGFHLIL"
     gene            complement(1794..3764)
                     /locus_tag="VFX11_16795"
     CDS             complement(1794..3764)
                     /locus_tag="VFX11_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940334.1"
                     /translation="MLIHKPRACTDTHFFRALKPAVVVLGEGGVVLDFSPGAEALFGL
                     RRSQLDRVGFGQCVPCPKEYHNDLTSYLLDYAESNPNLWHQPLVAYRYDGVRFPLVYR
                     IARVEGASPCWICEFVDAAEYQGNQHRLKQQLDETLASSRAKSRFLHHMSQQFRAPIG
                     RLLGAIGEALDYGQLPGALADNLEQALLSGKELQRSLNEMVDFTRLETDQLEFQNINF
                     NFRLVLEEIVDGFSDLAKRRGIELATLVSPYVPESVVGDPDRLHQILQSLLNNAFKFT
                     HEGGITVKAECQIENDTHAVIELEVTDTGEGMSEEKASAIRTAFEKRDASFSDRYGGL
                     GIGLAISKELLELMGGSISLRTVEGLGSTFKVSLKLPKGAGLEANSSLLSRHRLVLVS
                     DSLRDRPKLLEYFQEWQLEIESYGSSPVALEKMRQASLTGQPCELVVIDLQQPKLAGL
                     QLAQEINRIPAFANVRILLLVGDRDHLNLGAVPNLRVDAVLLKPVRKQMLHDAIATVL
                     GREQSAVQPLVTDRSLQDANGKQTRRALLVDDNEVNQIIAKGALKKLGIHADVTNNGE
                     EAIEAVMEKQYDFILMDCEMPIMDGFEATRSIREWEQQHGGHLPIIALTASDSGDCHD
                     ACMQAGMDEYMQKPFRADQLNTILNRIQIKPR"
BASE COUNT          758 a         1238 c         1160 g          863 t
ORIGIN      
        1 tccaggatca ggtggaagcc attcttgcgc agttgattca aaacctcgct ctgctggctg
       61 tggcgaatgg ccagcgagga ttccggcagg cccacataaa tctgttcggt gcgaataccc
      121 tgggcgcgga cgacccccgt cacccattcc accccccctt cctgcatcag cagcgacggt
      181 gacaccggca ggatcaactt cacgttctcg cgctggcggg cattcatggt actgacccgt
      241 tccagcgcgt gctgccaaaa ccagcggaaa atcaccggcg ccagaccgga ttcctccgcc
      301 gccggcagga attcgtccag catcagttca ccgcgcgccg gattcaccca cgacagtttg
      361 atctcacacg ccaccagctg gtcgttttcc actgccgcca ccggctggaa ctgcacgcgc
      421 cactgcttct gctcaacggc ctgatgcagt tcgtattcga tctgcatctg ctccacccgc
      481 tgcttgttca tttcagcggt gtagtactga taattgttgc gaccgatggc tttggcccgc
      541 gccagtgcgg attcggcatt tttcagcagc gtacgcgcgt catcgccaca ggtgggataa
      601 acgctcacgc caatcgagca tccggtgaat acctggtgac cgtcgatcag gaacgtctcg
      661 cggatctggt caatgatttt ctgcgccacg atgcccgcgt tgtccggcag atcgatatgg
      721 ccgagcacca ccacgaattc atccccttcc atgcggccca ccatgtcatc acggcgcacc
      781 agtgagttga tgcgctgcgc cacttcgcgg atcagctggt cgccgcgatc atgccccagg
      841 gattcgttga tgtagcggaa atggtcgagg tcaacataca gcaccgcaaa ataaaagccg
      901 gaaatgcccg ccttcaatac gcagcggtca atatgctcct cgatgggtgc ccgtagcggc
      961 aggttggtca ggtgatccac atgggaaagc ttggcgatct tgtctttcga ttcccgtgtg
     1021 tccttcagtt ggcggaaggc aaacagcaac ccaatctcgg gcagcgtgtt cacgggaatc
     1081 gccgcgaatt tcacgctgat actttcgccg tcggcgcgcc acagcatggc cttgtccacc
     1141 tgcaggatct gttctgtgcg cgtaactttt gcgatggggt gatccttcca gcgcgaacgc
     1201 acgccgctgc aggcttcttc cagaatggtt tccagataat tgcccgccag atccagcacc
     1261 ggcgctttca gcaggcgctc ggccgccgcg ttggcatata gaatctgacc ttgccggtcc
     1321 agggccagca ccccttcttc acggttttcc accagcgact ttttgttctc gctgcccatg
     1381 gccatgatct ggttgcggta ttgctgcagc tgcaggtaga tcttcactcg atgctgcagc
     1441 acgcgttcgg ccaccggctt ggccagcaca tccaccaccc ggaacagttc ctggtgcagg
     1501 cacatcttgc gctccgacag attgccggtg atgaacagca ccggaatgtg cagcgtgcgg
     1561 ctgtcggact tgagcaggtt caacacctgg taactgtcgg gatcgttcag cgcggaatcc
     1621 agaatcagca acgcgggctg gtggctggcc accagattca gtacgttgcc gccttccgcg
     1681 gtggccaggg cacgggcgcc ggaggcctgt accagttcgg aatagtaggc gatgttggcg
     1741 tcctggttgt cggcgatgat gatcaagggc agtgtctggt tcatatcatc cattcaacgg
     1801 ggtttgatct gaattcggtt cagaatcgtg ttcagctgat cggcgcgaaa cggcttctgc
     1861 atgtactcat ccataccggc ctgcatgcag gcgtcgtgac aatcgccgga atcgctggcg
     1921 gtaagggcga tgatgggcag atggccaccg tgttgctgct cccattcgcg aatgctgcgg
     1981 gtggcctcga agccgtccat gatcggcatt tcacaatcca tcaggataaa gtcgtattgt
     2041 ttctccatca ccgcctcgat ggcttcctca ccattgttgg tgacgtcggc gtggatgccc
     2101 agttttttca gggcgccctt ggcgatgatc tggttcactt cgttgtcgtc caccagtaac
     2161 gcgcgccggg tctgcttgcc gttggcatcc tgcaggctgc ggtcggtcac cagcggttgc
     2221 acggcagact gttcgcgccc cagcacggtg gcgatggcat catgcagcat ctgcttgcgc
     2281 accggtttca gcagcacggc atccacccgc aggttgggca cggcacccag attcaggtga
     2341 tcccgatcgc cgaccagcag caggatgcgc acattggcga atgcggggat acgattgatt
     2401 tcctgcgcca gctgcaggcc tgccagcttg ggttgctgca agtcgatcac caccagttcg
     2461 cagggctgtc ctgtcaggct ggcctggcgc attttttcca gcgccaccgg gctgctgcca
     2521 taactctcaa tttccagctg ccattcctgg aaatattcca gcagcttggg acggtcgcgc
     2581 agggaatcgg acaccagcac cagccggtgc cggctcagca gggaggaatt ggcctccagc
     2641 cccgcgccct tgggcaactt cagtgaaacc ttgaatgtgg agcccagccc ttccaccgta
     2701 cgcagcgaga tcgaaccgcc catcaactcc agcagttcct tgctgatggc caggccaatg
     2761 cccagcccgc cgtaacggtc ggaaaaagag gcatcgcgtt tttcaaaggc cgtgcggata
     2821 gcgctggcct tttcctccga catgccttcg ccggtgtctg tcacttccag ttcgatcacg
     2881 gcgtgggtgt cgttttcgat ctggcattcg gctttcaccg tgataccgcc ttcgtgcgtg
     2941 aacttgaatg cgttgttgag caggctttgc agaatctggt gcaggcggtc aggatcgccg
     3001 accacgcttt ccggcacata gggcgacacc agagtcgcca gttcgatgcc gcgccgcttg
     3061 gccagatcgc tgaagccgtc gacgatttcc tccagcacca ggcggaaatt gaaattgata
     3121 ttctggaatt ccagctggtc cgtttccagc cgggtgaaat ccaccatctc gttcagcgag
     3181 cgttgcagtt ccttgcccga cagcagggcc tgctccaggt tgtcggccag cgcgccgggc
     3241 aactggccgt aatccagtgc ctcgccgatg gcgccgagca gacggccaat gggcgcgcga
     3301 aactgttgcg acatgtggtg cagaaagcgc gacttggcgc gggacgacgc cagggtttca
     3361 tccagttgct gcttcaggcg gtgctggttg ccctggtatt cggcggcatc gacgaactcg
     3421 cagatccagc agggtgatgc gccctccacc ctggcgatgc ggtacaccag cggaaagcgc
     3481 acgccgtcat aacggtaggc caccaggggc tggtgccaca ggttgggatt gctttcggcg
     3541 taatccagca ggtaggacgt caggtcgttg tgatattcct tcgggcaggg cacgcactga
     3601 ccgaacccga cccggtccag ctggctgcgg cgcaggccga acagagcttc cgccccgggt
     3661 gagaaatcca gcaccacgcc accctcgccc agcaccacca ccgccggttt gagggcgcga
     3721 aagaaatgcg tgtccgtaca ggcgcgtggc ttgtgaatca gcatggggct ccaactgcta
     3781 aatgaccgtc atctcagtat agatgccact ggtcggccca ccagaaaagg cagggcttaa
     3841 atccgggcat aaaaaaagcc ccgcacatca cgtgggggct ttcttgacca gatctttgta
     3901 aaacaattac ttacttcaac ttacggccct tgctggcggc gatccgcatg cgcagcgcgt
     3961 tgagcttgat gaagccttcg gcatccttct ggttgtaggc gccgccgtcg tcctcgaag
//
LOCUS       DASUEG010000891         5093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1293470, whole genome shotgun sequence.
ACCESSION   DASUEG010000891 DASUEG010000000
VERSION     DASUEG010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293470"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFX11_16800"
     CDS             complement(<1..318)
                     /locus_tag="VFX11_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940335.1"
                     /translation="MSSRSFFQDGLSVFITKIVVIGLSIFSVVIQANALGPAGRGVLA
                     MLLIYPQLFTSIAEGGMRQATVYYVGQKAIPDARVFGASILYTLGSAAIFSTLIFFLL
                     STSE"
     gene            complement(359..1408)
                     /gene="murB"
                     /locus_tag="VFX11_16805"
     CDS             complement(359..1408)
                     /gene="murB"
                     /locus_tag="VFX11_16805"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX4940336.1"
                     /translation="MTNRVHDQTLPVQNSASGAARLIPPALVERLRALNGLDVLPDLD
                     LGRFSRWKIGGTADCVVCPRSTAALREVILLAREAGVPVTVIGLTTNLLFAQEGVRAL
                     VIHLGPQFAGMRITGNRVWGQAGVWVPGFARRAAKAGLSGIEHLAGIPGTLGGLICMN
                     GGSQRKGIGDHLVSATALDLDGTLHTYDRAQCGFAYRTSVFQQNHQIITEAEFEYETA
                     RDAKAVRREMKHILEDRRRKFPQKLPNCGSVFVSNPALYASVGPPGAVIEKCGLKGTT
                     RGGAEISHLHANFIVNRGGATTADVLYLIDLIRTRVQQETGCDLEAEAKYVDTLGRFS
                     PAHVAARQQAQEHIA"
     gene            complement(1445..2722)
                     /locus_tag="VFX11_16810"
     CDS             complement(1445..2722)
                     /locus_tag="VFX11_16810"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4940337.1"
                     /translation="MKADMIIEGTQVPAGKVVVSGAKNSATRLLAAALTSAETVRIRN
                     FPTHLVDAVAKIRFMRDLGADIEVDNAHSSVEIHTRSLSLRPDLDYNVPIRTTYLLAA
                     GQLARNGEARIPYPGGCKIGNRGYDLHIMVWQALGCVVTEEENCIHIQGRLRGNLIDF
                     PISTVGGTENALICAAVAMGDTEIRNAYVSPEVHDLILLLREMGAQIDNFGNSLIRVR
                     GCSGLLRGATFQVMPDRIEALTWIIFAVMTGGEICIDGVPFEHMQAPLLHLQEAGIDI
                     FRNSRSAYINANCVSNAGIQPFELACGTHPGVISDMQSFYTLLGMKAKGTSRIYDYRY
                     PERIAYARELDKFAPGAVACEPGKITTRGMKPMTGARVTSTDLRGSMALVMCAFCAEG
                     KSYVEGVDLALRGYNDLAGKLKSLGLNFQVIEA"
     gene            complement(2840..3709)
                     /locus_tag="VFX11_16815"
     CDS             complement(2840..3709)
                     /locus_tag="VFX11_16815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4940338.1"
                     /translation="MASDIPLITVVITAFNRPDYLEQSVASALAQEGADLEIIVIDDC
                     SPTDLQPVLAAFSGPIRYHRMARNGGANRARNEGVRQARGRYVAFLDDDDIWLPHKLQ
                     RQLQHLAGATACICGFRLLEKDLVFVHPIDQVNATMLKLGNPYCGMSGLLCERQWLLQ
                     NPFDENLDNGQDWDMFVRLARQAPLRYVAEPLFRYRRGSHDSITTKVRQMKPADMERR
                     LLVTYKHREWLGERLFRRRIAHTILGYLGTRSQPWKLVLMALRISGPLATATVLGEKF
                     NKFMRRGGRVSTH"
     gene            complement(3746..4879)
                     /locus_tag="VFX11_16820"
     CDS             complement(3746..4879)
                     /locus_tag="VFX11_16820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4940339.1"
                     /translation="MRVAFVGGRGYHSNHGGVENAMREIASRLAACPDLDVDVYGQGN
                     LPWFAATTEAPGLTIVGAPALCSRFDGNAVLALANCLYALLMRRPKVLLLFASGPSLL
                     AILARLLRVKVIAALRAIDSQRDKWGWFNASLLRLGEISALRVADACTVNSLEMFRYY
                     DGAARGLIYIPNGASAASAGSDAVLERLGLHPDGYLLFAARLDPVKRLHLLLRAHGRL
                     PTDCRLPLVVAGGQCTSAAYRQELEALSGTGVQFIGHVDGDILDPLMRHCAVFVLPSI
                     LEGMSNSLLEAMHSGRCVLCADVAANCDVVQQEPAALFRADDLDDLTQRLTDYCRHPE
                     QRRRCGQAMQQIACQHYCWDTTAERYRELIFRTAGWSTPATGT"
     gene            complement(4901..>5093)
                     /locus_tag="VFX11_16825"
     CDS             complement(4901..>5093)
                     /locus_tag="VFX11_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940340.1"
                     /translation="PLWQVEGMILFAILNALAWELNLTVMVPAPATTGPDRQVPLPTG
                     QVSVPDTRDTPVVPHQDRA"
BASE COUNT          901 a         1579 c         1644 g          969 t
ORIGIN      
        1 ttctgacgtg ctcagcagaa agaaaatcag ggtggagaaa atggcggcag atcccagcgt
       61 atacaggatg gacgcgccaa acacgcgggc gtccggaatg gccttctgcc ccacgtagta
      121 cacggttgcc tggcgcatgc caccctctgc gatcgaggtg aacagctggg gataaatcag
      181 cagcatggcc agcacgccac gcccggcggg gccgagtgcg ttggcctgga tcaccacgct
      241 gaagatcgac agcccgatta ccacgatctt ggtaatgaag accgaaaggc cgtcttgaaa
      301 aaaggatcgg gatgacacgg ttactccttg ctgcagccgg tagtggcgac aatcgttgtc
      361 atgcaatgtg ctcctgtgcc tgctggcgcg ctgccacatg ggcagggctg aaacggccca
      421 gcgtatccac atacttggct tccgcctcca ggtcgcagcc ggtttcctgc tgtacccggg
      481 tgcggatcag atcgatcaga tacagtacgt ccgccgtggt ggcgccgccg cggttgacga
      541 tgaagttggc gtgcaggtgc gagatctcag cgccgccccg cgtcgtgcct ttcagaccac
      601 atttttcgat caccgcgccc ggcgggccaa cactggcgta cagggcggga ttgctgacaa
      661 acaccgagcc gcagttgggc agcttctgtg gaaacttgcg ccggcgatct tccagaatgt
      721 gtttcatctc ccgccgcacc gccttggcat cgcgggcagt ttcatactcg aattcggctt
      781 ccgtgatgat ctggtggttc tgctggaaga ccgacgtgcg gtaggcaaag ccgcattgcg
      841 cccggtcgta ggtatgcagc gtgccgtcca gatccagcgc ggtggccgac accagatgat
      901 cgccgatgcc tttgcgctgg ctgccgccgt tcatgcagat cagcccgccc agggttcccg
      961 ggatgccggc aagatgctcg attccgctca gaccggcctt ggccgcgcgc cgggcaaagc
     1021 cgggtaccca cacacccgcc tggccccaga cccggttgcc cgtgatgcgc atgccggcaa
     1081 actgcgggcc caggtgaatc accagtgccc ggacaccttc ctgggcaaac agcaaattgg
     1141 tggtgaggcc gatcaccgtc accggcacgc ccgcttcccg tgccagcaga atcacctcgc
     1201 gcagtgcagc cgtcgaccgc ggacacacga cgcaatctgc tgtgccaccg attttccagc
     1261 ggctgaaccg gcccaggtcc agatccggga ggacgtccag cccgttcagt gcccgcaggc
     1321 gctccaccaa cgccggtggg atgaggcggg ccgcccccga tgcgctattt tggaccggaa
     1381 gcgtttggtc gtgcaccctg tttgtcatgt gagcgttttc tgtccacatt gatgcgtttg
     1441 cccctcaggc ttcgatcacc tggaagttca ggcccaggga tttgagcttg cccgccagat
     1501 cgttgtagcc gcgcagcgcc agatccaccc cttccacata gcttttgccc tctgcgcaga
     1561 aagcgcacat caccagcgcc atgctgccgc gcaggtcggt ggaggtcacc cgggcgccgg
     1621 tcatgggttt catgccccgg gtggtgatct tgccgggctc gcacgccacc gcgccggggg
     1681 cgaacttgtc cagttcgcgg gcgtaggcga tgcgttccgg atagcggtag tcatagatgc
     1741 gcgaagtgcc tttggccttc atgcccagca gggtgtagaa cgactgcatg tcggaaatca
     1801 cgcccggatg ggtgccgcag gccagttcga atggctggat gccggcgttg ctgacgcagt
     1861 tggcgttgat gtaggcgctg cgggagttgc ggaagatgtc gatgccggct tcctgcagat
     1921 gcagcagggg ggcctgcatg tgttcaaacg gaacaccgtc gatgcagatc tcgccgccgg
     1981 tcatgaccgc aaagatgatc caggtgaggg cttcgatgcg gtccggcatg acctggaagg
     2041 tggcaccgcg cagcaggccg ctgcagccgc gcacccggat gaggctgttg ccgaaattgt
     2101 cgatctgggc gcccatttcc cgcagcagca ggatcaggtc gtgcacttcc ggcgacacat
     2161 aggcgttacg gatttcggtg tcgcccatgg ccacggcggc gcagatcagc gcgttctcgg
     2221 tgccgcccac ggtggagatg gggaagtcga tcaggttgcc gcgcagacgg ccctggatgt
     2281 gtatgcagtt ttcttcttcc gtcaccacgc agcccagggc ctgccacacc atgatgtgca
     2341 ggtcgtagcc ccggttgccg atcttgcagc cgccggggta aggaatccgg gcctcgccgt
     2401 tgcgcgccag ctggccggct gccagcaggt aggtggtacg gatcggcacg ttgtaatcca
     2461 ggtccgggcg caggctgagg ctgcgggtat ggatctcgac gctggaatgg gcgttgtcca
     2521 cttcgatgtc ggcacccagg tcacgcatga agcgtatctt ggccacggcg tccaccagat
     2581 gggtgggaaa attgcggatg cgcacggttt ccgcggaggt gagggccgct gccagcagtc
     2641 gggtggcgga gttttttgca ccgctcacca ctaccttgcc ggctggaacc tgggttccct
     2701 cgatgatcat gtccgctttc attggttgac tccctttgga tactggcatt gcttgaatgg
     2761 aggaccggcc cgtgacggcc agtgccttca aatggatcac gtgtctggtt ttacaaagca
     2821 actaacgggc cgggtgcact cagtgggtgc tgacgcggcc gccccgccgc atgaatttgt
     2881 tgaatttttc gcctaatact gttgccgtgg ccaggggccc gctgatgcgc agtgccatca
     2941 agacaagctt ccagggttgg ctgcgggtgc cgagatatcc caggatggtg tgggcgatcc
     3001 ggcgccggaa cagacgctct cccagccatt cccggtgctt gtaggtcacc agcaggcgcc
     3061 gctccatgtc cgccggcttc atctgccgga ccttggtggt gatgctgtcg tggctgccac
     3121 gccggtaccg gaacagaggt tcggccacat agcggagggg ggcctgccgg gcgaggcgca
     3181 caaacatatc ccagtcctgg ccgttgtcca gattctcgtc gaacgggttc tgcagcagcc
     3241 actggcgctc gcacagcagg ccgctcatgc cgcagtaggg gttgcccagt ttgagcatgg
     3301 tggcgttcac ctggtcgatc gggtggacga agaccagatc cttctccagc agcctgaatc
     3361 cgcagatgca ggccgtggcg cccgccaggt gttgcagctg ccgttgcagt ttgtggggca
     3421 gccagatgtc gtcatcatcc aggaaggcca catatcggcc ccgggcctgg cgcacgccct
     3481 cgttgcgggc ccggttggcg ccgccgttgc gcgccatgcg gtggtagcgg atcgggcctg
     3541 aaaacgccgc caggaccggt tgcagatcgg tgggcgagca gtcgtcgatc acaatgatct
     3601 ccaggtcggc cccttcctgc gccagggcgc tggcaaccga ctgttccagg tagtccggcc
     3661 ggttgaaggc agtgatgacg acggtgatca ggggaatgtc gctggccatg gtttcgatag
     3721 gattctgctt caggttgtga gtgactcagg tgccggttgc cggtgtggac cagccggcgg
     3781 tacggaatat cagctcgcgg taacgttccg cagtggtatc ccagcaatag tgctgacagg
     3841 caatctgctg catggcctgg ccacaccggc gtcgctgctc cgggtggcga cagtagtcgg
     3901 tcaggcgttg cgtcaggtca tccagatcat cagccctgaa cagcgccgcc ggttcctgct
     3961 gcaccacgtc gcagttcgct gccacgtcgg cgcacagcac acagcggccg ctgtgcatgg
     4021 cctccagaag gctgttggac atgccttcca ggattgatgg cagtacaaat actgcacagt
     4081 gacgcatgag cgggtcgagg atatccccgt cgacgtggcc gataaattgt actcccgtgc
     4141 cggatagtgc ctccagttcc tgacgatagg ctgccgaggt gcactggccg ccggcaacta
     4201 ccaggggcag ccgacaatcc gtcggcaacc gcccatgggc ccgcaacagc agatgcaggc
     4261 gcttgaccgg atcgagccgg gcggcaaaca gcaggtagcc atccgggtgc aggcccagcc
     4321 gctccagtac ggcatcgctt ccggcgctgg cggcactggc gccgttgggg atgtagatca
     4381 ggcccctggc ggcgccatcg taatagcgga acatctccag gctgttcacc gtgcaggcat
     4441 cggccactcg cagggcgctg atttccccca ggcgcagcag actggcattg aaccagcccc
     4501 acttgtcgcg ctggctgtcg atggcgcgca gggccgctat cactttcacc cgcagcaatc
     4561 gcgccagaat ggccagcagg ctgggcccgg aggcaaacag caacagcacc tttggccgcc
     4621 gcatcagcag tgcatacagg cagttggcca gtgcgagcac ggcgttgcca tcgaagcggg
     4681 agcagagtgc aggggcgccc acgatggtca ggccaggggc ctcggtggtc gctgcaaacc
     4741 agggcaggtt tccctgtcca tagacatcta catcgagatc cgggcaggca gccagccgcg
     4801 aggcgatttc acgcatcgcg ttctccactc cgccatgatt ggagtgatat ccccggccgc
     4861 ctacgaatgc cactctcact tcccatctcc tggtcgcgca ttacgccctg tcctggtgag
     4921 gaacgacagg cgtgtccctg gtgtccggca cactgacctg gccggtcggc aagggtacct
     4981 gacggtccgg tcctgtcgtg gccggcgcgg gtaccatcac ggtcaggttc aattcccatg
     5041 ccagtgcatt gaggatcgcg aacagtatca tgccttcgac ctgccacaag ggc
//
LOCUS       DASUEG010000892         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_210857, whole genome shotgun sequence.
ACCESSION   DASUEG010000892 DASUEG010000000
VERSION     DASUEG010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210857"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFX11_16830"
     CDS             complement(<1..629)
                     /locus_tag="VFX11_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4940341.1"
                     /translation="MSYQHILDGKTLDLPVGKVVCVGRNYAEHAKELNNPVPTSPILF
                     IKPSTALVRLEGGFVIPKDQGECHHELEMSVLIGKPLKNADVATVKAAILGYGIGLDL
                     TLRDLQNRLKEKSHPWEVAKAFDGACPLTAFVAAEKIGGPEQADIALRVNGAVRQTGN
                     TSDMITPVFALIAFMSRHFTLLPGDVIMTGTPAGVAALHAGDQLQVTLGT"
     gene            complement(626..2023)
                     /locus_tag="VFX11_16835"
     CDS             complement(626..2023)
                     /locus_tag="VFX11_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4940342.1"
                     /translation="MTDTTRIIAGLTQLVDAGRLLTDAASLKHYGCDWTKKYDPQPLA
                     IALPKTVEQVQAIVRFANEHKLGLVPSGGRTGLSAAAVAANGEVVVAFDLMNKVLDFN
                     ATDRTARCQAGVVTEQLQQFAEEQGLYYPVDFASAGSSQLGGNISTNAGGIKVIRYGM
                     TRDWVAGLKVVTGKGDILDLNADLIKNATGYDFRHLFIGAEGTLGFIVEATMKLARAP
                     KNLTVMVLGVPEFRAIMQVLNAFQSKIDLTAFEFFSEKAVQLVTAHHKVPRPFDTATP
                     YYALLEFEAASDGVMEEAMALFESCVENGWASDGVISQSETQAAALWRLREGISETLA
                     PFIPYKNDISVVVSKVPDFLEDIDAIVGPQYPDFEIVWFGHIGDGNLHLNILKPQALS
                     KEEFFKRCETVNTQVFETVQKYNGSVSAEHGVGLTKKPYLHYTRSAAEIGYMKAVKQV
                     FDPNNVMNPGKVFDL"
     gene            2385..3614
                     /gene="serA"
                     /locus_tag="VFX11_16840"
     CDS             2385..3614
                     /gene="serA"
                     /locus_tag="VFX11_16840"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX4940343.1"
                     /translation="MTATSLDKSKIRILLLEGVHQSAVDNFKRAGYTNIEYIKTALSG
                     DELKAKVADAHFVGIRSRTQLTEEIFAAAPKLVAVGCFCIGTNQVNLNSALIRGIPVF
                     NAPFSNTRSVAELTLAQAILLLRRVPEKNASCHRGGWLKDAKNSFEIRGKTLGIIGYG
                     NIGSQLSVLAEGLGMKVRFYDVVTKLPMGNASQVNTLDELLEEADVVSLHVPETPSTK
                     NMFGRDQLAKMKKLSILINNARGTVVDIDALAEALREQRILGAAIDVFPKEPKSNDEE
                     FITPLREFDNVILTPHIGGSTLEAQQNIGSEVSEKFIKYSDNGSTISAVNFPEVALPV
                     HGNVHRLLHIHKNVPGMLSKINKIFSDNNINICGQYLQTNDQIGYVVIDVDKASSELA
                     LEKVREVEGTIRARVLY"
BASE COUNT          782 a         1187 c         1024 g          738 t
ORIGIN      
        1 gtcccgagcg tcacctgcaa ctgatcaccg gcatgcagcg ccgccacacc cgccggcgta
       61 cccgtcatga tcacatcgcc gggcagcagg gtgaaatgcc ggctcatgaa ggcaatcaat
      121 gcgaacaccg gtgtgatcat gtcggacgta ttgcctgttt gccgcaccgc accgttcact
      181 ctcaacgcaa tatcggcctg ttccggcccg ccgatttttt ccgccgccac aaacgccgtc
      241 agcgggcagg cgccgtcaaa ggctttcgcc acttcccacg gatgggattt ttccttcagc
      301 ctgttctgca gatcccgcaa ggtcaggtcc aggccaatgc cgtaacccag aatcgcggct
      361 ttcaccgttg cgacatccgc atttttcagc ggtttgccga tcagtaccga catttccagt
      421 tcatgatggc actcaccctg atctttcgga atgacaaagc caccttccag ccgcaccagc
      481 gccgtggacg gtttgataaa caggatcggc gacgtgggca ccggattgtt cagttccttc
      541 gcatgctcgg cgtaattgcg gccgacacac accaccttgc ccaccggcag gtccagggtt
      601 ttgccatcca gaatatgctg atagctcaca ggtcaaatac cttgccggga ttcatcacat
      661 tgttgggatc gaacacctgc ttcaccgctt tcatgtaacc gatctcggcg gcactgcggg
      721 tgtaatgcag gtacggcttc ttggtcagac ccacaccatg ctcggccgac acactgccgt
      781 tgtatttctg caccgtctcg aacacctgcg tgttcacggt ttcgcagcgt ttgaaaaatt
      841 cctctttcga cagcgcctgc ggcttcagta tattcaggtg caggttgccg tcgccgatat
      901 ggccgaacca cacgatctcg aaatccggat actgcggacc gacgatcgcg tcgatgtctt
      961 ccagaaaatc cggcaccttc gacaccacta ccgaaatgtc attcttgtac ggaatgaagg
     1021 gcgccagggt ttcactgatg ccttcccgca gccgccacaa tgccgctgcc tgtgtctcgc
     1081 tctggctgat cacgccgtcg ctggcccagc cgttttccac gcaggattca aacagcgcca
     1141 tggcctcttc cattacaccg tcgctcgccg cttcaaactc cagcagcgcg taatagggcg
     1201 tggcagtgtc gaacgggcgc ggtaccttgt gatgcgctgt caccagctgc acggcttttt
     1261 cggaaaaaaa ttcaaacgcg gtgagatcga ttttgctctg gaaggcattc agcacctgca
     1321 tgatggcgcg gaactccggc acgcccagca ccatcacggt gagatttttc ggcgcccgcg
     1381 ccagcttcat ggtggcttcc acgataaagc ccagcgtgcc ttcagcaccg atgaacagat
     1441 ggcggaaatc atagccggtg gcgttcttga tcaggtcggc attcaggtcg aggatgtcgc
     1501 ccttgcccgt caccactttc aggcccgcca cccagtcgcg agtcatgccg taacggatca
     1561 ccttgatgcc gccggcgttg gtggaaatat tgccgcccag ctggctggaa ccggccgagg
     1621 caaaatccac cggataatac aggccctgct cctcggcgaa ctgctgcagc tgctcggtga
     1681 ccacgccggc ctggcaacgc gcggtgcggt cggtggcgtt gaagtccagc accttgttca
     1741 tcaaatcgaa cgccaccacc acttcgccat tggccgccac cgccgccgcc gacagcccgg
     1801 ttctgccgcc ggaaggcacc agtcccagct tgtgttcatt ggcaaagcgc acgatggcct
     1861 gcacctgctc cacggttttg ggcagggcga tggccagggg ttgagggtcg tattttttgg
     1921 tccagtcgca gccgtagtgt ttgaggctgg cggcgtcggt aagcaggcgg cctgcgtcca
     1981 ccagctgggt cagtcctgca atgatccggg ttgtgtcagt catgggattt ctcttttgga
     2041 gcaagggttt ggaaactgcc agggacgctg gcggtgaacg ctgtccagac acgccgtgaa
     2101 cccatccctg ggggctgcac agcggcatcc ctgccgctga gcgtccggcc agcgttcacc
     2161 gccagcgtcc cgtccaaatt cggtgccacg tttcgtagtg ccaaaaccgc accattttac
     2221 cgaccccaag gtcatttccc ccattaggga aattcaccgc gccttgcgac attttcaagg
     2281 ccccggggat gtgaacaaag ggggttatga taacataggc cgcctttcgg ggctggtccc
     2341 caccagctgc caccggttct tttcaagacg taagcgagac catcatgacc gcgacatccc
     2401 tggacaaaag caagatccgc atccttctgc tggaaggcgt acaccagtcg gccgtcgaca
     2461 acttcaagcg cgccggctac accaacatcg aatacatcaa gaccgccctg tccggggacg
     2521 aactgaaagc caaggtcgcc gacgcgcact tcgtgggcat ccgctcccgc acccagctca
     2581 ccgaggaaat cttcgccgcc gccccgaaac tggtggccgt gggctgcttc tgcatcggca
     2641 ccaaccaggt caacctgaat tccgccctga ttcgcggcat tcccgtcttc aacgcgccct
     2701 tctccaacac ccgttcggtg gcagaactga cactggcgca ggcgatcctg ctgctgcgcc
     2761 gcgtgccgga aaagaacgcc agctgccacc ggggcggttg gctgaaagac gccaagaaca
     2821 gcttcgagat ccgcggcaaa accctcggca tcatcggcta tggcaacatt ggttcgcagc
     2881 tcagcgtgct ggccgaaggc ctgggcatga aagtgcgctt ctatgacgtg gtgaccaagc
     2941 tgccgatggg caatgcatcg caggtcaaca ccctggacga actgctggaa gaagccgacg
     3001 tggtgagcct gcacgtgccg gaaaccccgt ccaccaaaaa catgttcggt cgcgaccagc
     3061 tggcgaaaat gaaaaagctg tcgatcctga tcaacaacgc ccgcggcacc gtggtggaca
     3121 tcgacgccct ggccgaagcc ctgcgtgaac aacgcattct gggcgccgcc atcgacgtgt
     3181 tcccgaaaga gcccaagtcc aacgacgagg aattcatcac cccgctgcgg gaattcgaca
     3241 acgtgatcct gacaccgcac atcggtggca gcacgctgga agcgcagcag aacatcggct
     3301 cggaagtgtc ggagaagttc atcaagtaca gcgataacgg ctccaccatt tccgccgtga
     3361 atttccccga agtggcgctg ccggtgcacg gcaatgtgca ccgcctgctg cacatccaca
     3421 agaacgtgcc gggcatgctg tcgaagatca acaagatttt ctccgacaac aacatcaaca
     3481 tctgcggaca gtacctgcag accaacgacc agatcggtta tgtggtaatt gatgtagaca
     3541 aggcgtccag cgagctggcg ctggagaaag tgcgcgaagt ggaaggcacc atccgggcgc
     3601 gcgtgctgta ctgatccgca atgctgccgt gacaaacaaa aaacccgctg ccaacacagc
     3661 gggttttttt gtgccttgcc cagcggcagg ccgccatgcg caggcattgg ttgcaattcc
     3721 atgacagcga t
//
LOCUS       DASUEG010000893         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1669081, whole genome shotgun sequence.
ACCESSION   DASUEG010000893 DASUEG010000000
VERSION     DASUEG010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669081"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFX11_16845"
     CDS             <1..616
                     /locus_tag="VFX11_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940344.1"
                     /translation="SMVVGHSQTGEMDKADGPYYLFRVMRQLQRWLPRWLPLVGLEGG
                     YFNMVPVDFVARALDRIAHLPALDGQCFHLTAHRNYSLGELMNIVADVAHAPRAAWQI
                     SNQWLEQPPGRLVKAATTTGPVRWLAQKALQRLEVPVAMLSFLTYPVTFATTNTDAAL
                     AGTDIQAPDLETYIRPLWDYWNEQLEPAQPPRHFRHHFRTERHD"
     gene            609..1610
                     /locus_tag="VFX11_16850"
     CDS             609..1610
                     /locus_tag="VFX11_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940345.1"
                     /translation="MTDKVKFRKPKILPYFQRAADHWKWNAKSIISPEQALADRVSGK
                     VVMLTGASSGIGALVAHKLAAAGATVLMTARSKDKLQDLAEEIRNAGGKADVYAGNLT
                     VMEDCDRICQEALRDHGRVDILINNAGRSIRRSIKFSFDRFHDYERTMQLNFFGSIRM
                     AMNLLPGMLERDEGHIINVSTVGVQANPARFSAYLSSKWALEGWSWVAANEFAHTGVR
                     FSTINYPLVRTPMIAPTKIYDYMPVMSPETAVKWMLDVIITRNKRKLGLLGKGALAMY
                     YVFPKTSESIVNASYQVVYEAPPASYAESREKLKAVQPETPPTLPNASEQAASTVRH"
     gene            complement(1854..>2468)
                     /locus_tag="VFX11_16855"
     CDS             complement(1854..>2468)
                     /locus_tag="VFX11_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX4940346.1"
                     /translation="AIVVLIVMNVRGVSGVAPYILVGLVLWTAVLKSGVHATLAGVIL
                     GLIIPATGRDQHGRNTLEQLEHDLKAPVGFVILPLFAFANTGLSLAGLTFASLLHPVP
                     LGIALGLFLGKQVGVFGLCFVAAKLRLVSLPSGVNWKQMYGVSILCGIGFTMSLFISS
                     LAFTGGDPNFALNDRLGVLLGSLLSAVLGYGILRWVLPSAVTKG"
BASE COUNT          555 a          799 c          718 g          396 t
ORIGIN      
        1 gtccatggtg gtgggccaca gccagaccgg cgagatggac aaggctgacg gcccctatta
       61 tctgttccgg gtcatgcggc agctgcagcg ctggctgccc cggtggctgc cactggtggg
      121 tctggaaggt ggctacttca acatggtgcc ggtggatttc gtcgcccggg ccctggaccg
      181 gatcgcacac ctgcctgcac tcgacggcca gtgctttcat ctcaccgcgc accgcaatta
      241 ttccctgggc gaactgatga atatcgtcgc agacgtggct cacgcaccgc gcgccgcctg
      301 gcagatcagc aaccagtggc tggagcagcc gccgggccgc ctggtgaaag ccgcaaccac
      361 caccggcccc gtacgctggc tggcgcagaa agcgctgcag cgtctcgagg tgcccgtagc
      421 gatgctgtcg ttcctgacct acccggtaac gtttgctacc accaacaccg acgcggccct
      481 ggccggcacc gacattcagg cgccggatct tgagacgtat atccggccac tgtgggatta
      541 ctggaacgaa cagctggaac ccgcgcagcc accccgacat ttccgtcatc acttccggac
      601 cgaacgccat gactgacaaa gtgaaattca gaaagccgaa aatcctgccc tatttccagc
      661 gcgcagccga tcactggaag tggaacgcga agagcatcat cagccccgaa caggcgctgg
      721 cagaccgcgt gtccggcaag gtggtgatgc tgaccggcgc cagctccggc atcggcgcac
      781 tggtggccca caagctggca gcggccggcg ccaccgtgct gatgacggca cgctcgaaag
      841 acaaactgca ggatctggcg gaagaaatcc gtaacgcagg tggcaaggct gatgtctacg
      901 ccggcaacct gacggtgatg gaagactgtg atcgcatctg ccaggaagcg ctgcgggatc
      961 atggccgggt ggacatcctg atcaacaatg cgggccgttc catccgtcgt tcgatcaaat
     1021 tcagtttcga ccgcttccac gactatgagc gaaccatgca gctgaacttt ttcggttcaa
     1081 tccgcatggc gatgaacctg ctgccgggca tgctggagcg cgacgaaggc cacatcatca
     1141 acgtgtccac cgtgggcgtg caggcgaatc cggcgcggtt ttccgcgtac ctgtcgtcga
     1201 aatgggcgct ggaaggctgg agctgggtgg cggccaacga atttgcccac acgggcgtgc
     1261 gcttctccac catcaactac ccgctggtgc gcacgccaat gatcgcgccc accaagatct
     1321 acgattacat gccggtgatg tcgccggaga cggcggtgaa atggatgctg gacgtgataa
     1381 tcacccgcaa caagcgcaag ctgggactgc tgggcaaggg cgcactggcc atgtactacg
     1441 tgttcccgaa aacgtcggaa agcatcgtca atgccagtta ccaggtcgtg tacgaggcgc
     1501 cgccggcctc ttacgcggaa agccgggaaa aactgaaagc agtgcaaccg gaaacgccac
     1561 ccacgctgcc taacgccagc gaacaggcgg catccaccgt gcggcattga aatccatcag
     1621 gatattgccg gggcgacgca cgcaagtccg cgccccggca atagggctca gacgccgccg
     1681 atctcgcaca catcgttacc cagccacagg gccagatcgc acgtggtgca gccggacagc
     1741 agcagggacg acagtaaaac cagcacagcc attcttgttg ttttcatggt gcagttcccg
     1801 atccagagga aggttgaatc ccgatcatac acggaagcgc aagcgcgcct ccatcagcct
     1861 tttgttaccg ccgacggcaa cacccaccgc agtatcccat aacccagcac tgccgacagc
     1921 agactgccca gcaggactcc caagcggtca ttcagggcga agttgggatc accgccggta
     1981 aacgccagcg agctgatgaa cagactcatg gtgaaaccga taccgcacag gatcgacacg
     2041 ccgtacatct gcttccagtt gacgccggac ggcagcgaca ccagccgcag cttcgccgca
     2101 acgaaacaca ggccgaacac gccgacctgc ttgcccagaa acaggcccaa tgcaatgccc
     2161 agcggtaccg gatgcagcag cgaagcaaac gtcagccctg ccagcgacag gccggtattg
     2221 gcgaaggcaa acagcggcag aatcacgaag cccaccggcg ctttcaaatc gtgttcgagc
     2281 tgctccagtg tgttgcggcc gtgctggtcg cggccggtgg cgggaatgat caggcccaga
     2341 atcacaccgg ccagagtcgc atgcacgccg gatttcaaca ccgccgtcca cagcaccagt
     2401 cccaccagaa tatagggcgc cacgccactc acaccgcgca cattcatcac aatcagcacc
     2461 acaatcgc
//
LOCUS       DASUEG010000894         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1870561, whole genome shotgun sequence.
ACCESSION   DASUEG010000894 DASUEG010000000
VERSION     DASUEG010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870561"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1161
                     /locus_tag="VFX11_16860"
     CDS             112..1161
                     /locus_tag="VFX11_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4940347.1"
                     /translation="MLAGCNLALAATFDLPTDGSTVVGAPRVVIPIGDNTLLDIARKL
                     DVGHHEITWANPGVDVWMPDDAERVVVPSQFILPPKPWTGIVLNISQRRLFYFPAAAK
                     GQPAKVITFPVSIAREGWSTPLGVTKVVAKHKDPAWFVPKSIQAEHKADTGEEFPEYF
                     PPGPDNPMGMLAIQLGFKSIFIHGTNRPWGVGMRTSHGCLHLYPEDAAELFPLMKAGI
                     PVRVIDEPVVAGVSNGQLHLASYEPVAEYGTTRDLMTRAVLALAPFLQASVQPLPDPQ
                     AKPAKAAPVPRYTVDWDKVKASIASPQVLPLSIAPEGISLAQKLEAVTPEVYEYPPYG
                     VDANNAALPAEPAIE"
     gene            1229..1825
                     /locus_tag="VFX11_16865"
     CDS             1229..1825
                     /locus_tag="VFX11_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX4940348.1"
                     /translation="MTKPFSQACENNQQPILDVLRRHVSAPGTLLEIGSGTGQHAVFM
                     ARHLPQLQWQPTDVPANLPGIDAWRTEAGLGNVAPPLAFDVWDATPSLTPSRYLFSAN
                     TLHIMSWETVQRFFSLIPSLLEPDGIAFFYGPFNYNGIFTSDSNARFDQWLKAQAPHQ
                     GIRDFAAVAQLAEQAGMALIEDCPMPANNRTLVWRRYL"
     gene            complement(1816..2898)
                     /locus_tag="VFX11_16870"
     CDS             complement(1816..2898)
                     /locus_tag="VFX11_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4940349.1"
                     /translation="MTSFAPITGTVGTESTSIPQPDTLNPAGILATGFGFWSSKVLLT
                     AVDLGVFTRLAGTRLRGEELGQILGLHPRGIWDFFDALVALGFLQRSGNGVDALYFNS
                     AETEQFLDRNKPTYVGGILEMANDRLFRFWSDLKPALQTGQPQNEVRHSQKPMFEVLY
                     EDAPRLEQFMGAMRGISAGNFAAFAEKFDFSRYQSLCDVGGATGLLSSLVAQRHAHLH
                     CTTFDLPAVEPIARQWIARAGLEQRVDVVSGDFLTGTLPRADIITMGMILHDWNLEKK
                     KHLIRLAYDALPEGGAFVAIENLIDNERRENAFGLMMSLNMLIEFGDAFDFTAADFEG
                     WCREAGFRRFDVLHLNGPCSAAIAYK"
     gene            3044..>3858
                     /locus_tag="VFX11_16875"
     CDS             3044..>3858
                     /locus_tag="VFX11_16875"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4940350.1"
                     /translation="MDQLYCMRVFTRVVEQGSFARAADDLAVSRATITTAVAQLEQHL
                     GSRLLHRTTRRLSLTDEGQRYYASCVRILGELAEADDELSRRTVRGLLRVSVPQSFAR
                     LPFFPALEAFLRQHPDLSVDVVLTDRAVNLVEEGIDCAIRGAAIPGDSLLVARQLTPV
                     NRATCASPAYLEQHGTPADLAALASHDCIRFISPATGRPAPWIFNVAGESQEFVPAGR
                     LAVTSYEAAAAAAEAGIGIVQVPEPLVYRELRAGRLRPLLEDFTAHMPPLCVV"
BASE COUNT          718 a         1101 c         1271 g          768 t
ORIGIN      
        1 aggtttttga gatggaatgc taaccggcag caggcagtag gcatgggcaa tacaacaggt
       61 ttttataaca ggatgatgag gcagtgtagg cgatggctgc ccggcttggc gatgctggca
      121 ggatgcaatc tggcactggc ggctacgttc gatctgccca ccgatggttc cactgttgtc
      181 ggcgcgcccc gggtagtgat tcccatcggc gacaatacgc tgctggatat cgcccgcaag
      241 ctggatgtgg ggcaccacga aatcacctgg gccaatcctg gcgtggatgt gtggatgccg
      301 gacgatgccg agcgtgtggt ggtgccgtcg caattcattc tgccgcccaa gccctggacc
      361 ggcattgtgc tgaacatttc ccagcgccgt ctgttttatt ttcccgctgc cgccaaaggc
      421 cagccggcga aggtgatcac gtttccggtc agcatcgcgc gggagggctg gtcgacgcca
      481 ctgggtgtga ccaaagtggt ggccaagcac aaggacccgg cctggtttgt gcccaagagc
      541 atccaggcgg aacacaaggc tgataccggt gaggagtttc cggaatattt tccgccgggg
      601 ccggacaatc ccatgggcat gctggcgatc cagctgggat tcaagagtat tttcatccat
      661 ggcaccaacc ggccctgggg ggtgggcatg cgcaccagcc acggttgtct gcacctgtat
      721 ccggaagatg cggccgaact gtttccgctg atgaaggcgg gtattcccgt gcgggtgatc
      781 gatgagccgg tggtggcggg cgtcagcaat ggccagttgc atctggccag ttacgaaccg
      841 gtggctgaat atggaaccac gcgggatctg atgacgcggg cggtactggc attggcgccg
      901 ttcctgcagg cgtcggtgca gccgttgcct gatccgcagg ccaaacctgc gaaggcagcg
      961 cctgtgccgc gttacacagt ggattgggac aaagtgaaag cgtctattgc atcgccgcag
     1021 gtgctgccgc tctccattgc gccggagggg atcagcctgg cgcagaagct ggaggcggtg
     1081 acacccgagg tttacgagta tccgccctac ggcgtcgatg cgaacaatgc agcgctgccg
     1141 gcggaacccg ccatcgagta aacggatctg gcgctgcggg aacttatgac gaaatattca
     1201 ggacgaaacg ttttgtgaga aaccttctat gacgaaaccg ttttcgcagg cctgtgaaaa
     1261 caaccagcaa ccgattctgg acgtattgcg ccggcatgtg agtgcgcccg gcacgttgct
     1321 ggaaatcggc agcggcactg gtcagcacgc ggtgttcatg gcccggcacc tgccgcagtt
     1381 gcagtggcag cccacggatg tgccggcgaa tttgccgggc attgatgcct ggcgcaccga
     1441 agcgggtctg ggcaatgtgg caccacctct ggcgttcgat gtgtgggatg ccacgccgtc
     1501 cttaactcca tcccgctacc tgttttccgc caacaccctg cacatcatga gctgggaaac
     1561 ggtgcagcgt tttttcagtc tgattccatc gttgctggag ccggacggca tcgctttttt
     1621 ttacggcccg ttcaattaca acggtatttt taccagcgac agcaacgcgc gtttcgacca
     1681 gtggctgaaa gcgcaggcgc cgcatcaggg catccgtgat tttgctgcgg tggcccagct
     1741 ggcggagcag gcgggaatgg cgttgataga agattgcccc atgcccgcca acaatcgcac
     1801 gctggtgtgg cgacgctatt tgtaggcgat ggcggcgctg caggggccgt tcaggtgcag
     1861 cacgtcgaag cgacggaaac cggcctcgcg acaccagcct tcaaaatcgg cggcggtgaa
     1921 atcgaaggcg tcgccgaatt cgatcagcat gttcagcgac atcatcaggc cgaaagcgtt
     1981 ttcccgccgc tcgttgtcga tcaggttttc gatggcgacg aaggccccgc cttccggcag
     2041 cgcgtcatag gccagccgga tcaggtgttt tttcttttcc agattccagt cgtgcaggat
     2101 catgcccatg gtgatgatgt cggccctggg cagggtgccg gtgaggaaat cgccggacac
     2161 cacatccacc cgctgttcca ggcctgcccg cgcaatccac tgccgcgcga tcggttccac
     2221 cgcgggcaga tcgaacgtgg tgcagtgcag gtgcgcatga cgctgtgcca ccagactcga
     2281 cagtaatccg gtggcgccac ccacatcgca cagcgattgg tagcgggaga aatcgaattt
     2341 ttccgcgaag gcggcaaagt tgccggcgga gataccgcgc atcgcgccca tgaactgctc
     2401 cagccggggc gcgtcttcat acagcacttc gaacatgggt ttctggctgt ggcggacttc
     2461 gttttgcggc tggccggttt gcagtgccgg cttcagatcg ctccagaagc ggaacaggcg
     2521 atcgttggcc atttccagaa tgccacccac gtaggtgggt ttgttgcggt cgagaaattg
     2581 ctcggtttcg gcgctgttga aatacagcgc atcgacgccg ttgccgctgc gctgcagaaa
     2641 gccgagcgca accagtgcat cgaaaaaatc ccagatgcca cggggatgca ggcccaggat
     2701 ctggcccagt tcctcgccgc gcaggcgggt gcctgccaga cgggtgaaca cgcccaggtc
     2761 gacagcggtg agcaggactt ttgaactcca gaagccgaag ccggtggcga gaatgccggc
     2821 cgggttcagg gtgtcgggct ggggaatgga agtggattcg gtgccaacgg ttccggtgat
     2881 aggggcaaag ctggtcatgg cgggttctcc ctgagtgcca aaagccaaaa gtatcggatt
     2941 gaaactgcaa cggtgtgcac tctagtgccc tgccggggcg ggataaagct ggcaagcctg
     3001 aattgattgt ttatgatctc caaacaatcc gcggaggaat gtcatggacc aactctactg
     3061 catgcgtgtg ttcacccggg tggtggagca gggttcgttt gcacgcgccg ccgatgatct
     3121 ggcggtgtcg cgggccacga tcaccacggc agtggcacag ctggaacaac atctgggcag
     3181 ccgtctgctg caccgcacca cccgccgcct gagcctgacc gacgaaggcc agcgctacta
     3241 cgccagttgc gtgcgcatcc tgggggaact ggcggaggcg gatgatgaat tgtcccgccg
     3301 cacggtgcgc ggcctgttgc gggtatcggt gccgcaatcg tttgcgcgcc tgccgttttt
     3361 tccggcgctg gaggcatttt tgcggcagca tccggacttg tcggtggacg tcgtgctgac
     3421 ggatcgcgcc gtcaatctgg tggaagaggg cattgactgt gcgatacgcg gcgccgcgat
     3481 tccgggcgac tcactgctgg tagcgcgaca gctgacgccg gtgaatcggg ccacctgcgc
     3541 ctcgccggcg tatctggagc agcacggcac gcctgcggat ctggcggcgc tggccagcca
     3601 cgattgcatc cgcttcattt cccccgctac cgggcgaccg gcgccctgga ttttcaacgt
     3661 tgcgggtgag tcgcaggaat ttgtgccggc ggggcggctg gcggtgacgt cgtacgaagc
     3721 ggctgccgca gcggcggaag cgggcatcgg catcgtgcag gtgccagaac ctctggtgta
     3781 ccgggaattg cgtgcgggca ggttgcggcc cctgctggag gatttcacgg cgcacatgcc
     3841 gcccctgtgc gtggtgta
//
LOCUS       DASUEG010000895         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1519440, whole genome shotgun sequence.
ACCESSION   DASUEG010000895 DASUEG010000000
VERSION     DASUEG010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519440"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..487)
                     /locus_tag="VFX11_16880"
     CDS             complement(77..487)
                     /locus_tag="VFX11_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4940351.1"
                     /translation="MQPRMSMITLGVADLERSVRFYRDGLGFPKMDSPPEVAFFTLSG
                     TWLALYPRDLLAADAHVPAQGSGFPGFTIAHNVDSEAQVDEVIGKAVGAGATLLKPGQ
                     KVFWGGYSGYFSDPDGFLWEIAHNPLFWVGPRDS"
     gene            complement(519..1088)
                     /locus_tag="VFX11_16885"
     CDS             complement(519..1088)
                     /locus_tag="VFX11_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HEX4940352.1"
                     /translation="MSVSNREELVEHLRHGNRVHFLFFWGHQKSAAGIGKTCLSQWYE
                     SPFEAGGITYLTAEQFMMAEKARLFGDGDTRAKILASTRPDTAKALGRVVAGFDDRRW
                     AERRFDIVVAANLEKFQQNPDLKAFLLGTGDSVLVEASPVDSIWGIGLAADDPNAGNP
                     AAWKGLNLLGFALMQVRDRLRRQTDVTAV"
     gene            complement(1134..1571)
                     /locus_tag="VFX11_16890"
     CDS             complement(1134..1571)
                     /locus_tag="VFX11_16890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4940353.1"
                     /translation="MFEVKCSVDIPRPPDVVFAFAGNYANDPVWRSGVLSMVYQTSGA
                     PAAGVRTKEIIRSMGRKAVTIGEIIEYSPSRTAFRSISGPVPCEGSREFVPVASGTRF
                     TYSLTLHPTGFLGLLEPLLKRMFTKQIQADVQRLKRHLEKNST"
     gene            complement(1659..2141)
                     /locus_tag="VFX11_16895"
     CDS             complement(1659..2141)
                     /locus_tag="VFX11_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4940354.1"
                     /translation="MSIEIRIADYRNPADARDIGFLLNLYASDKMGGGEPLPQHVQDN
                     VARELSRLPYAFSLLCHVDGVPAGLVNCFEAFSTFACKPLINIHDIAISGEFRGRGIS
                     QKLLAAVEEIARRKGCCKITLEVLEGNIVAKNAYRKFGFDNYALDPEMGHAEFWQKSL
                     "
     gene            complement(2194..>2311)
                     /locus_tag="VFX11_16900"
     CDS             complement(2194..>2311)
                     /locus_tag="VFX11_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HEX4940355.1"
                     /translation="SFSPVNYGYKRLSDLIRASELFDMEMRKTVMYFRDARK"
BASE COUNT          500 a          696 c          640 g          475 t
ORIGIN      
        1 ccatttttag cgcctagagt acccacggag cgtatgtgag tatggtccag ccagcgcttt
       61 tttgcacaat aagtggtcag gaatcccgag gcccaaccca aaacagcgga ttgtgggcga
      121 tctcccagag aaaaccatcc ggatcggaaa agtagccgga atagccgccc caaaacacct
      181 tctgccccgg cttgagcagc gttgcacccg cgcccaccgc cttgccgatc acctcatcca
      241 cctgggcttc tgaatccacg ttgtgggcga tcgtgaagcc cgggaagccg gaaccctgtg
      301 ccggcacatg ggcatctgca gcgagcaaat cccgcggata aagcgccagc caggtgccgc
      361 tcagtgtaaa gaacgccacc tcgggcggcg aatccatttt gggaaaaccc agtccgtccc
      421 ggtagaagcg gacagaccgc tccagatcgg cgacacccag cgtaatcatg ctcattcgcg
      481 gctgcatagc ctgttgtact ccctgtggaa aacgcggttc agacagcggt gacgtctgtt
      541 tggcgacgaa gccggtcccg cacctgcatc agggcaaaac ccagcaggtt cagtcctttc
      601 caggcagcgg gatttcctgc attgggatca tcggccgcca acccgattcc ccatatgctg
      661 tcgaccgggc tggcctccac cagcacactg tcacctgtac ccagcaggaa tgccttcagg
      721 tcgggattct gctggaattt ttccagattg gcagccacta caatgtcgaa gcgccgttcg
      781 gcccaacgcc ggtcatcgaa tcctgccaca actcggccta gcgccttggc agtatcgggg
      841 cgggtggacg ccagaatttt cgcccgcgtg tctccgtcgc caaacagcct ggctttttca
      901 gccatcataa attgctcagc tgtcaaatag gtgatgccgc cggcctcaaa gggggattca
      961 taccactggc tgaggcaggt cttgccgatc cccgcggcgg atttctggtg tccccagaaa
     1021 aacaggaagt ggaccctgtt gccatggcgg agatgttcga ccagttcttc ccgattgctg
     1081 acgctcacac aattaccccg ctgaaaaatg gctgccggat ttcaatgaag aggtcaggtg
     1141 gaattctttt caagatgccg cttcaaccgc tgtacatcgg cctggatctg tttggtaaac
     1201 atgcgtttga gcaacggttc aagcaggccg agaaaacccg tgggatgaag ggtcagggaa
     1261 taggtgaaac gagtccccga agccaccggc acgaactcac ggctgccttc acagggcacc
     1321 ggacctgaaa tgctgcgaaa cgccgtacgg gaaggcgagt attcaatgat ttcgccgatg
     1381 gtgacggcct tgcgtcccat gctgcgtatg atctccttgg tacggacgcc tgccgcgggc
     1441 gcgccgctgg tctggtacac catggacagc accccgctgc gccacacagg gtcattggca
     1501 tagttgccgg cgaatgcgaa aaccacgtcg ggcgggcgcg gtatgtccac ggaacatttc
     1561 acttcgaaca tgggatctcc tgatttctga gatagcggcc tgggtgtcaa cggacgtaga
     1621 acgctgccgg caaccccgcc gggcgcgtat gagcctgcct acagcgattt ctgccagaac
     1681 tcggcatgcc ccatttccgg atcgagcgcg tagttgtcaa agccgaattt gcgataggca
     1741 ttctttgcca caatattgcc ttccagcact tccagcgtga tcttgcagca acccttcctg
     1801 cgcgcgatct cttcaaccgc tgccagcaat ttctggctta tgccgcggcc gcgaaattcg
     1861 ccggaaatcg caatatcatg gatgttgatc agcggcttgc aggcgaaggt ggaaaaagcc
     1921 tcgaagcagt tcaccagccc ggccggaaca ccgtccacat gacagagcag gctgaaggca
     1981 taaggcaacc gggacagctc acgggccacg ttatcctgca catgctgcgg caggggttcg
     2041 ccgccaccca ttttatcgga cgcgtaaagg ttcaacagga acccgatgtc tctggcatcg
     2101 gcaggattcc ggtaatcggc tatgcggatt tcgatactca tgctgctgga tctgccctct
     2161 ggattgtgtg cggatcgcgc cggctgccat cgcttacttg cgcgcatccc tgaaatacat
     2221 cacggttttt ctcatttcca tatcgaacag ctcgctcgcc ctgatcaggt cgctgagccg
     2281 cttgtaaccg taattgaccg gtgaaaagga g
//
LOCUS       DASUEG010000896         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_170010, whole genome shotgun sequence.
ACCESSION   DASUEG010000896 DASUEG010000000
VERSION     DASUEG010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170010"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFX11_16905"
     CDS             complement(<1..1286)
                     /locus_tag="VFX11_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4940356.1"
                     /translation="MLSKDLEVTLNLAFRDARTKRHEYMTVEHLLLALLDNDAAATVL
                     KACGTNLDELRSDLANFVDETTPLIPSSDSERETQPTLGFQRVLQRAVFHVQSSGKKE
                     VTGANVLVAIFSEQESQAVYFLKKQSVARIDVVNYIAHGISKVSDESHNLEDHSQETH
                     EEEGVSEGTGKSPLESYATNLNEQAAVGRIDPLVGRDEEVERTVQILCRRRKNNPLLV
                     GEPGVGKTAIAEGLAKKIIDSEVPEAISDCVVYSLDLGALLAGTKYRGDFEKRFKALL
                     AELKKKKHAILFIDEIHTIIGAGAASGGVMDASNLLKPMLASGDIKCIGSTTFQEYRG
                     IFEKDRALARRFQKIDVVEPSVDDTYEILKGLKSRFEEHHDLKYSNKALRTAAELAAR
                     YINDRHLPDKAIDVIDEVGAYQRLQPPSKRKKVITVA"
     gene            complement(1325..1660)
                     /gene="clpS"
                     /locus_tag="VFX11_16910"
     CDS             complement(1325..1660)
                     /gene="clpS"
                     /locus_tag="VFX11_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX4940357.1"
                     /translation="MENTGGDTPHRDFDGGLAVAESKADLAPPPMYQIVMLDDDFTPM
                     EFVVDVLQQFFGMNREKATQIMLTVHTQGRASCGTFTRDVAETKAAQVIKYARENQHP
                     LMCEVEKYQ"
     gene            1964..2272
                     /gene="cspD"
                     /locus_tag="VFX11_16915"
     CDS             1964..2272
                     /gene="cspD"
                     /locus_tag="VFX11_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014109107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HEX4940358.1"
                     /translation="MATGKVKWFNNAKGYGFIRPDTGGEDLFVHYSYICMDGYRSLKA
                     GQQVTYDVREAPKGLHAMNINMIGAEQKSPTQETTQAEPFGSAAAGASSESMLHQEAI
                     "
     gene            complement(2421..3683)
                     /gene="icd"
                     /locus_tag="VFX11_16920"
     CDS             complement(2421..3683)
                     /gene="icd"
                     /locus_tag="VFX11_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX4940359.1"
                     /translation="MGYQKIRVPADGDKITVNADLSLNVPNHPIIPFIEGDGIGVDIT
                     PVMLKVTNAAVAKAYGNKRSITWMEIYCGEKSTKVYGPDIWLPEETLEALREYVIGIK
                     GPLTTPVGGGIRSLNVALRQELDLYVCQRPVRWFRGVPSPVHCPEKTNMVIFRENSED
                     IYAGIEWKADSEEAKKVIRFLREEMGVKKIRFPETCGIGIKPVSKEGTQRLVRKAIQY
                     TIDNDCPSLTLVHKGNIMKYTEGAFKDWGYELAVERFGAEPVDGGPWHQFKNPKTGKT
                     IIVKDVIADAFLQQILMRPDEYSVIATLNLNGDYISDALAAEVGGIGIAPGANIGGSV
                     AVFEATHGTAPKYAGQDKVNPGSLVLSAEMMLRHMGWTEAADLIIKGMQGAIEAKTVT
                     YDFERLMPDATLLRCSEFGDAIVEHMDD"
BASE COUNT          870 a         1096 c          959 g          953 t
ORIGIN      
        1 gcaaccgtga tcactttctt gcgcttgctc ggtggctgca gacgctgata ggcacccact
       61 tcgtcgatca cgtctatcgc cttgtccggc agatgacgat cattgatgta gcgagctgcc
      121 agttcggccg cagtacgcag ggccttattg gaatacttca gatcgtgatg ttcttcgaag
      181 cgcgatttca aacctttcag aatttcgtag gtgtcatcca cgctgggttc aaccacatcg
      241 atcttctgga agcgacgcgc cagggcacgg tctttctcga aaataccgcg gtattcctgg
      301 aatgtggtgg aaccaatgca cttgatgtcg cccgatgcca gcatcggctt gagcaggttc
      361 gacgcatcca tcaccccgcc agatgctgca ccggcaccga tgatagtgtg gatctcgtcg
      421 atgaacagaa tcgcgtgctt tttctttttc agttcggcca gcaaagcctt gaagcgtttt
      481 tcaaaatcac cgcgatattt ggttccggcc agcaatgcgc ccagatccag cgaatacacc
      541 acgcaatcag aaatcgcttc cggcacttcg ctgtcgatga ttttcttcgc cagaccctcg
      601 gcaatggcgg tcttgcccac accgggctcg cccaccagca gcgggttgtt cttgcggcga
      661 cggcagagaa tctgcacggt gcgctcaacc tcttcgtcac gcccaaccag cggatcgata
      721 cgacccactg cggcctgctc attcaggttg gtggcatagg attccagcgg actcttgccg
      781 gtaccctcgg aaacaccctc ttcctcatga gtttcctggc tgtgatcttc caggttgtgc
      841 gattcgtccg acaccttgga aatgccgtga gcgatgtaat tcaccacatc gatgcgtgcg
      901 acgctctgct tcttcaggaa atagacagcc tggctttcct gttcgctgaa aatcgccacc
      961 agcacgttgg cacccgtgac ttccttcttg ccggaggatt gcacgtgaaa caccgcccgc
     1021 tgcaaaaccc gctgaaagcc caatgttggc tgggtttcgc gctcggaatc gctggaggga
     1081 atcagcggag tggtttcatc aacgaagttg gccaggtccg accgcaattc gtccagatta
     1141 gtgccgcacg ccttgagcac agttgctgcg gcgtcattgt ccagcaatgc gagcagcaga
     1201 tgttcaaccg tcatatattc atgacgcttg gtacgtgcat ctctgaacgc caaatttaac
     1261 gtgacttcca gatctttgct taacatcttc tttacacctt aatcctgcac taaggatgat
     1321 cgggctattg atacttttcc acttcacaca tcaagggatg ttggttttcc cttgcgtatt
     1381 tgatcacttg cgcggctttt gtttccgcga catcccgggt aaatgtaccg cagctggcac
     1441 gtccctgggt gtgaaccgta agcatgatct gtgtagcctt ttcacgattc ataccaaaaa
     1501 actgctgcaa cacatccaca acaaactcca tcggagtgaa atcgtcgtcc agcattacga
     1561 tctggtacat cggcggtggc gccagatctg ctttcgactc tgcaacagcc agaccaccat
     1621 caaaatcacg atggggggtg tcgcctcccg tgttctccat atgaataaat ggtagtagag
     1681 aatctttggc tttactcata accagaaatc ataaaatcca tggataaaat actgagcgct
     1741 cataaagagc atatatgggg gctacgttgg tcagttcaac cccaaaaaca cttgactaat
     1801 acgccacaat cgcgatgatt gtaggctgtg attaacaaaa gttaacacaa aatactccag
     1861 cacagtacaa ttcttcgtaa ttcgctgttt ggctggataa aaataataac aatcgacacg
     1921 actgccgctg cgatgaggta gtcccaacag aaggacttga gttatggcaa ccgggaaagt
     1981 gaagtggttc aataacgcca agggttacgg ctttattcgt ccagataccg gaggtgagga
     2041 tctcttcgtg cattactcct acatttgcat ggatggatac agaagcctca aagcgggaca
     2101 gcaagtcacc tacgacgttc gcgaagctcc caaaggcctg catgcgatga acatcaacat
     2161 gataggcgca gaacagaaat cccccaccca ggaaaccaca caggcggaac ctttcggctc
     2221 ggctgccgct ggcgcatctt ccgaaagcat gctgcatcag gaagcgatct agctttctgt
     2281 acctcacacc ctgcccgcag gcggcagggt cggatagagc ggaaactgca gcgccagacg
     2341 cagtttttcc cgcttttctc cgctattaac atcaaacggc gcccattggc gccgtttgat
     2401 gaaagcccgt ttccggtcgt tcagtcatcc atgtgctcta caatggcgtc gccgaattcg
     2461 gagcaacgca gcaatgtggc atctggcatc aaccgctcga agtcataggt tacggtcttg
     2521 gcttcgatgg cgccctgcat ccccttaatg atcaggtctg cggcctcggt ccagcccata
     2581 tgacgcaaca tcatttctgc agacaggacc agagaaccgg gattcacctt gtcctggcca
     2641 gcatacttgg gcgcagttcc gtgagtagcc tcgaacaccg cgacactgcc gccgatgttg
     2701 gcgcccggag caataccgat gccccctact tccgccgcca gcgcgtccga gatgtagtcg
     2761 ccgttcaggt tgagcgtggc gatgacactg tactcgtccg ggcgcatcag gatctgctgc
     2821 aggaaggcat ctgcaatcac gtccttgacg ataatggtct tgccggtttt cgggttcttg
     2881 aactggtgcc agggaccacc gtccacaggc tcggcaccga atcgctcaac ggccagttca
     2941 tagccccaat ccttgaacgc gccttcggtg tatttcatga tgttgccctt gtgcaccagg
     3001 gtcagcgatg gacaatcgtt gtcgattgtg tactgaatcg ccttgcgcac cagacgctgg
     3061 gtgccttcct tggagaccgg cttgatgccg atgccacagg tttcggggaa acggattttc
     3121 tttacgccca tttcttcccg caggaagcga atcacttttt tggcttcttc gctgtccgcc
     3181 ttccattcga taccggcgta gatgtcttcg gaattctcac ggaaaataac catgttggtt
     3241 ttttccgggc agtgtaccgg gctgggcaca ccgcggaacc agcgcaccgg gcgctgacac
     3301 acgtaaagat caagctcctg acgcagcgcc acgttcagcg aacggatgcc tccgccaacc
     3361 ggcgtggtca agggaccttt gatgccgatg acgtattcgc gcagcgcttc cagggtttct
     3421 tcgggcaacc agatatcggg cccgtaaact ttggttgatt tttccccgca gtagatttcc
     3481 atccaggtga tggaacgctt gttgccatat gcttttgcaa cagcagcatt cgttactttc
     3541 agcatcactg gtgtgatatc gacgccaatc ccgtctcctt cgatgaaagg gatgatagga
     3601 tggttcggga cattcaggga caggtccgcg ttgacggtga ttttgtcacc atctgccgga
     3661 accctaatct tttggtaccc catgtcgttt tttactccct atttacatac aattacggtt
     3721 taggtacgcg aattatacag aagattgtat gcattcgcct catcaatttc gcgattactg
     3781 accaattatc aatcacataa tgcgccttcc gcccgccgct gaaccacagg tgttgctgct
     3841 caacaagccc tttcaggtgc tctgtcaatt ctccgaac
//
LOCUS       DASUEG010000897         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1404332, whole genome shotgun sequence.
ACCESSION   DASUEG010000897 DASUEG010000000
VERSION     DASUEG010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404332"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..3155
                     /locus_tag="VFX11_16925"
     CDS             105..3155
                     /locus_tag="VFX11_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940360.1"
                     /translation="MSFNLSEWALRNRQIVLYFMLVLAVAGAMSYTRLGQSEDPPFTF
                     KAMVIRSDWPGASAEEVSRQVTERIEKKVMETGEYQYVMSYSRPGESVVTFVALDNLN
                     NRKIPDLWYQVRKKVGDIRHTLPPGVIGPFFNDEFGTTFGNIYALTGDGFDYAVLKDY
                     ADRLQLQLQRVKDVGKVELIGLQDEKIWIELSNVKAATLGLSMAQVQQALEEQNAVSS
                     AGFFETRSDRVQLRVSGRFESVEEIRNFPIRVGDRTFHIGDVATVRRGFNDPPAPRMR
                     FMGKDGIGIAVSMKAGGDILVLGKALREESARLQNTLPVGMELHSVSDQPAAVKTGVG
                     EFVRVLVEALVIVLVVSFASLGLRTGLVVALSIPLVLAMTFAAMHYFEIGLHKISLGA
                     LVLALGLLVDDAIIAVEMMAVKMEQGFSRLKAASFAWTSTAFPMLTGTLITAAGFLPI
                     ATAQSGTGEYTRSIFQVVTIALLASWVAAVVFVPYLGDRLLPDIMGKHGGHAHDPYST
                     TFYRRVRAVVEWCVRRRGLVILITLALFVGSLALFRLIPQQFFPPSGRLELVVDLKLA
                     EGSSLKATEEQMKRLEEKLKTQPGVDNYVAYVGTGSPRFYLPLDQQLPSSRFGQFVVR
                     CKTVEEREDVRRWLIDTMQQEFPTLITRISRLENGPPVGYPIQLRVSGEHIDVVRDLA
                     RQVAAKVRENPYVSSVHLNWEEPSKVIYLNIDQERARALGVTTAALSRFLQSALSGSR
                     VNQFRENNELIEIMLRGTDRERQRMELLPSLAVPTDKGLSVSLSQVATVEYGFEEGVI
                     WHRDRLPTVTVYGDIYGGQQPSALVKDILPTLDPVRAQLPEGYRLEVGGTVEDSARGQ
                     ASVMAGMPLFVVVVMTLLMLQLKSISRSVLVFLTAPLGLVGVTWFLLAFQKPFGFIAM
                     LGTIALSGMIMRNSVILVDQIEQDIAAGIDRWQAVVGATVRRFRPIVLTALAAVLAMI
                     PLSRSNFWGPMAVAIMGGLISATVLTLLFVPALYAAWFRVRKPV"
BASE COUNT          526 a          966 c         1064 g          606 t
ORIGIN      
        1 ctgggtagtg gaggcgggcg tgcacatcct gctgccgggc cagcaggtga aaccggtgga
       61 ccgcgacaac cgtccggtgc agctggcggc ggggggataa cccgatgtct ttcaacctgt
      121 cggaatgggc gctgagaaat cgccagatcg tgctgtattt catgctggta ctggccgtgg
      181 cgggcgcgat gtcctacacc cggctgggtc agagtgaaga cccgcccttt acgttcaagg
      241 ccatggtcat ccgttccgac tggcccggtg ccagtgccga ggaagtgtcg cgccaggtca
      301 ccgaacgcat cgaaaaaaag gtgatggaga ccggcgaata ccagtacgtc atgtcctatt
      361 cccgcccggg tgaatcggtg gtgacctttg tcgctctcga caacctcaac aatcgcaaga
      421 ttcccgatct gtggtaccag gtgcgcaaga aagtggggga tatccgccac acgctgccgc
      481 ccggcgtcat cggacctttc ttcaatgacg aattcggtac caccttcggc aatatctacg
      541 ccctgaccgg cgacggcttc gattatgccg tgctgaagga ctacgccgac cgcctgcagc
      601 tgcaactgca gcgggtgaag gacgtgggca aggtggagct gatcggcctg caggacgaga
      661 agatctggat cgagctgtcc aacgtcaagg ccgccacgct tggcctgtcg atggcgcagg
      721 tgcagcaggc gctggaggaa cagaacgcgg tgtcctccgc cggctttttt gaaacccggt
      781 ctgaccgggt gcagctgcgg gtgtccggcc ggtttgagtc ggtggaggaa atccgcaatt
      841 tcccgatccg ggtaggtgac cgcactttcc atatcggcga cgtggccacc gtgcggcgcg
      901 gtttcaacga tccgccggca ccgcgcatgc gcttcatggg caaggacggc atcggtattg
      961 ccgtatccat gaaggcgggc ggcgacatcc tggtactcgg caaggccctg cgcgaggaat
     1021 ccgcccggct gcagaacaca ttgccggtgg gcatggaact gcacagcgtg tcggaccagc
     1081 cggcggcggt gaaaaccggc gtgggcgagt tcgtgcgggt actggtggaa gcgctggtga
     1141 tcgtgctggt agtgagtttc gcgtcgttgg gcctgcgcac aggtctggtg gtggcgctgt
     1201 cgattccgct ggtgctggcg atgacctttg cggcgatgca ttactttgag atcggcctgc
     1261 ataagatatc cctgggtgca ctggtgctgg ccctgggcct gctggtggac gatgccatca
     1321 tcgcggtgga aatgatggcg gtgaagatgg agcagggctt cagccgcctg aaggccgcca
     1381 gtttcgcctg gaccagcact gcgtttccca tgctcaccgg taccctgatc acggcggcgg
     1441 gcttcctgcc cattgccact gcccagtccg gcactggcga atacacccgc tccatattcc
     1501 aggtggtgac cattgccctg ctggcatcct gggtggcggc ggtggtgttc gtgccctacc
     1561 tgggcgaccg cctgctgccg gacatcatgg gcaagcacgg cggccacgcc cacgatccct
     1621 attccacaac gttctaccgg cgggtgcggg ccgtggtgga gtggtgcgtg cgccggcgcg
     1681 gtctggtcat cctgatcacg ctggccctgt tcgtgggttc gctggcactg ttccggctga
     1741 taccgcaaca gttcttcccg ccctcgggcc ggctggaact ggtggtggac ctgaagctgg
     1801 cggaaggctc ttccctcaag gccaccgagg agcagatgaa gcggctggag gaaaaactca
     1861 aaacccagcc tggcgtcgac aattacgtgg cttacgtggg caccggatcg ccccggttct
     1921 acctgccgct ggaccagcag ttgccgtcca gccggttcgg ccagttcgtg gtgcgctgca
     1981 aaaccgtcga ggaacgtgag gacgtgcggc gctggctgat cgacaccatg cagcaggaat
     2041 ttcccaccct gattacccgc atctcgcggc tggagaatgg cccgccggtg ggctacccga
     2101 tccagttgcg ggtgtcgggc gagcatatcg acgtggtgcg cgacctggcc cggcaggtgg
     2161 cggccaaggt gcgggagaat ccctatgtct ccagcgtgca cctgaactgg gaagagccca
     2221 gcaaggtgat ctacctgaac atcgaccagg agcgggcgcg tgcgctgggc gtcaccacgg
     2281 ccgcgctgtc gcggttcctg cagagcgcgc tgtccggctc ccgcgtcaac cagttccgcg
     2341 agaacaacga actcatcgag atcatgctgc gcggcaccga ccgcgagcgc cagcgcatgg
     2401 aactgctgcc cagcctggcg gtacccaccg acaagggcct cagcgtgtcg ctgtcccagg
     2461 tcgccaccgt tgaatacggt ttcgaggaag gcgtgatctg gcaccgcgac cgcctgccca
     2521 ccgtcaccgt ctatggcgac atttatggcg gccagcagcc gtcggcgctg gtgaaggaca
     2581 tcctgcccac gctggacccg gtgcgggccc agttgccgga aggctaccgg ctggaagtgg
     2641 ggggcacggt ggaggattcc gcccggggtc aggcctcggt gatggcgggc atgcccctgt
     2701 tcgtggtggt ggtgatgacc ctgctcatgc tgcagctgaa aagcatctcg cgttcggtgc
     2761 tggtgttcct caccgcgccc ctgggcctgg tcggcgtcac ctggttcctg ctggcattcc
     2821 agaagccatt cggcttcatc gccatgctgg gcaccatcgc cctcagcggc atgatcatgc
     2881 gcaattcggt gatcctggtg gaccagatcg agcaggatat cgcggccggc atcgaccgct
     2941 ggcaggcagt ggtgggcgcc accgtgcgcc gcttccgccc catcgtgctg acggccctgg
     3001 cggcggtgct ggcgatgatt ccgctgtcgc gctccaattt ctgggggccg atggcggtgg
     3061 cgatcatggg cggactgatt tccgccacgg ttctcacgct gctgttcgtg ccggcgctgt
     3121 acgctgcctg gtttcgcgtc aggaaaccgg tctgatcaag ga
//
LOCUS       DASUEG010000898         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1978412, whole genome shotgun sequence.
ACCESSION   DASUEG010000898 DASUEG010000000
VERSION     DASUEG010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978412"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFX11_16930"
     CDS             <1..343
                     /locus_tag="VFX11_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4940361.1"
                     /translation="QRMFPVQKVRFMYYTGEFIDAQEAWRLGAVERVVPKEKLRETAL
                     TIAAKIAEKSSPMIKLAKEALNGIEDGNLEDKYRWEQGFTLQAYSEKDSQEARNAFVE
                     KRDAEFSGQKS"
     gene            365..1522
                     /locus_tag="VFX11_16935"
                     /pseudo
     CDS             365..1522
                     /locus_tag="VFX11_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            1538..2605
                     /locus_tag="VFX11_16940"
     CDS             1538..2605
                     /locus_tag="VFX11_16940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940362.1"
                     /translation="MDFTFTDDQLLFCKSVQAFLQQEVTPAAVRQSWELDTGRSDERW
                     QQLVDLGVTAMLVPEQFGGLGMNELDFVLLAQACGQVALPEPLVETALVATPLLATLA
                     QRDSRCASLLEKIATGERKVAIGHAANNLVSDAHTADFLLLPSGNQIHLVPRDQVKLA
                     AQKSVDPSRRLFAVEWNANDETCLVSGPEGVALLAATLNRGALGAAAQLIGLAEAMVQ
                     MTVKYTSDRHQFGKPIGSNQALKHHMANCAVKIEFAKPTLYRAAYTVSKRPVHADFAV
                     SHAKVAAGEAALLAAKNSIQSHGAMGYTWECDLHIFMKRAWALDKFWGHAGFHKSRLH
                     QWLMNPNAKIGADKTFGEQVA"
BASE COUNT          601 a          791 c          855 g          503 t
ORIGIN      
        1 gcagcgcatg ttcccggtgc agaaagtccg cttcatgtac tacaccggcg aattcatcga
       61 cgcgcaggaa gcctggcgtc tgggcgcagt ggaacgcgtg gtgccgaaag aaaaactgcg
      121 cgaaaccgcg ctgaccatcg ccgcgaaaat tgccgagaaa agttcgccca tgatcaagct
      181 ggcgaaagaa gcgctcaatg gtattgaaga cggcaacctg gaggacaaat accgctggga
      241 acagggtttc accctgcagg cctacagcga aaaggattcg caggaagccc gcaatgcctt
      301 cgtggaaaaa cgtgacgccg aattttccgg ccagaaaagc tgaattgctg aaaggtattc
      361 catcatggat ctgaattaca ccgcagaaca gatcgccttt cgcaaggaag tgcgcagctg
      421 gctggagcag catgttcccg cccggcccct gcaatccttc gacacgaagg aaggttttga
      481 gcagcaccgc cagtgggaac aaaccctggc caaaggcaac tggggcatgg tgacctggcc
      541 gaaggaatac ggtggccggg gctgcaacct gatcgagtgg ctgatttttg aggaagaata
      601 ttaccgcgcc caggcgccgc tgcgggtcaa ccagaacggc attttcctgc tgggccccac
      661 gctgatggaa tacggcacgc cggaacagaa ggcgcgcttc atcaccaaaa tggccagcgg
      721 tgaggaaatg tgggcgcagg gctggtcgga gccgggtgcc ggcagtgaca tggcagcgat
      781 tcgctcgaaa gcgattcgtg acggcgacga gtacgtcatc aacggccaga aagtctggtc
      841 cacccgcgcg gtgtttgccg attgggtatt ctgcattttc cgcaccgatc cggcatccga
      901 caaacaccac ggtctgtcgt ttattttcgt gccgctgaat gcacccggcg tcaccgtgcg
      961 gccgattccg caattgaatg gtctgcccgg tttcgccgaa atttttttcg acgacgtgcg
     1021 cgtgccggtg gccaaccggc tgggcgacga agggcagggc tggaatatcg ccatgtccac
     1081 cgccggcttc gagcgtggcc tgatgctgcg ttcgcccgcc cgcttccagg aaaccgcgaa
     1141 acgactggtg gccctgtacc gcgaacaccg caatgacatc gtggatctca gcatagaaga
     1201 cgccgtggtg aaatccttca tggatgcaga agcctacgcg cttgccacct accagactgc
     1261 ttcgcggctg atgaatggcg gcaagattgg gccggagtct tctgtgaaca agattttctg
     1321 gtcggaactg gatcagcaca tgcacgaaac cgccatgagt attctggcgg cgcgggcgga
     1381 actgctgccg cactcttctg cggctggcga tgtgggcacc tggctggacg gtttcctgtt
     1441 tgcccagtcc ggccccattt atgccggcac caacgaaatc caacgcaaca tcattgccga
     1501 gcgcatgctg ggcatgccgc gcaaagagga gtgcagtatg gactttacct ttaccgatga
     1561 ccagctgctg ttctgcaaga gcgtgcaggc atttctgcag caggaagtga cgcccgcagc
     1621 agtgcgccaa tcctgggagc tggacaccgg ccgcagcgat gaacgctggc agcagctggt
     1681 cgacctgggt gtaaccgcca tgctggtgcc ggaacagttc ggcggcctgg gcatgaacga
     1741 actggatttc gtgctgctgg cccaagcctg cggtcaggtt gcgttgccgg agccgctggt
     1801 ggaaaccgcg ctggtggcca caccgctgct ggcaaccctg gcgcagcgtg attcccgctg
     1861 tgcctcgctg ctggaaaaaa ttgccacggg cgagcgcaag gtggccatcg gccacgccgc
     1921 caataatctg gtgtctgacg cacacacggc ggattttctg ctgctgccgt ccggtaacca
     1981 gattcatctg gtacctcggg atcaggtgaa gcttgcggcg cagaaaagtg ttgatccttc
     2041 ccgccgcctg ttcgcggtgg agtggaatgc caacgacgaa acctgtctgg tgagtggccc
     2101 cgaaggtgtt gccctgctgg cggccacact caatcgcggc gcactgggcg cagcggcgca
     2161 actgattggc ctggcggaag ccatggtgca gatgacggtg aaatacacat cggatcgcca
     2221 ccagttcggc aaacccatcg gcagcaacca ggcgctgaaa catcacatgg ccaactgcgc
     2281 cgtgaaaatc gaattcgcca agcccacgct ctatcgcgct gcatacactg tcagcaagcg
     2341 tccggtacat gcggacttcg ccgtgtcgca cgccaaggtt gcagcgggtg aagccgcgtt
     2401 gctggcggcg aaaaacagca ttcaatccca tggcgcgatg ggttacacct gggaatgcga
     2461 cctgcacatc ttcatgaagc gcgcctgggc actggacaaa ttctggggcc acgccggatt
     2521 ccacaagagc cgcctgcatc aatggctgat gaatcccaat gcaaaaatcg gcgccgacaa
     2581 aacgtttggt gagcaggtgg cgtaagcaga agaaagaatg taccgcgatg cgggaagaag
     2641 ggtgcggtgc tggcgtgttg ccagccaatc cacgcagccg agcatcgcag aattgtccgg
     2701 aggaaggtgg cgagtgtctg agcgcgcagc gcgagttgcg ccaccgccgg
//
LOCUS       DASUEG010000899         5028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1691490, whole genome shotgun sequence.
ACCESSION   DASUEG010000899 DASUEG010000000
VERSION     DASUEG010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691490"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3066)
                     /locus_tag="VFX11_16945"
     CDS             complement(<1..3066)
                     /locus_tag="VFX11_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940363.1"
                     /translation="MMFPNLSSFAVKQRSVTLYFILLSVLAGAYAFASLGRAEDPAIT
                     LQMVVVSAVWPGATPAEIERQVVLPIEKKIQEVDYVEEINTTVKADRADLQVKFYSYT
                     PMDKLPELQFQLRKRMLDIAASLPKGVIGPLVNDDFSDVYFSLYSLSAPGLPMREVAR
                     FAERLRDELQLISGVRKAMLIGEREERIFIDLDNVKMTNSGISSQTVFETIQAYNTLI
                     PSGQIDTAGPRLRFRLDADLSALQRLETLPIRTGTTVVHLGDLARVYQGYEEPPSYMV
                     RVNGQDALLIGVVMAKGENGLDLGERLAAFLQKEKAELPLGMTLDQITNQADAITAAV
                     NLFQVKFIVAVVVVMGVGCLALGFRAGLIVGIAVPITLGLTFVLMKSSGINLDRITLG
                     ALIIALGLLVDDAIIAIEIMLVKLEEGASKMTAATHAWTATASPMLFGTLITMFGFVP
                     IGFAKSGVGEYAGNIFWVLAFSLLISWLVAVTFTPYLGVQLLAKPRHDAEKKDHHGVY
                     DSPFYSRFRTVVDVCVRRRGAVVLATVTLFVLAVAGMAGAVEKQFFPGSDRPEILIDL
                     SLPPSTSIAVTDATVKRIEHLLKDMDGVKNYASYIGGGSPRFFISVNPEHPDSSFAKV
                     VVTAAGPHERDRIMRALENQVEAGAFPEARVRIRQLLFGPPVDWPVSFRVMGRDTATL
                     RELAGKVREVMLQHPHVVDPHLEWNQRVPTLKLNMDDERLRLLGMTPRDVAQQIQYRL
                     QGLPVTEVRQGIRTVDLLVRGQGGTQSPLDMPIYEVRNNAGQKIPLSHLGTTELVYED
                     AVIRRHQRQLFLAVQAEVKGAQAQDVTKAVWNDLQPLRAALLEGYRIDIGGSLERSKE
                     SEDSINQVFPLMLALMITCVMMQTRTFTGTLIVLATAPLGVIGAVIALLVFQKPFGFV
                     ALLGLIGLAGILMRNTLILTQQVSDNLADGLTPRDAVIEAAVRRARPVVLTALAAALA
                     FIPLTTDVFWGPMAYVLIGGVVAGTVITLLFVPALYALAFRFSAKEDSGN"
     gene            complement(3063..4223)
                     /locus_tag="VFX11_16950"
     CDS             complement(3063..4223)
                     /locus_tag="VFX11_16950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940364.1"
                     /translation="MARSLPQSMGRPGVAACDFGSDVISVRSRSLLVRGLLFMRLILF
                     IALLVLLGGCKAPSDSGVTAPPVKSVYTVTLTEQSQILPQSYSGTVRARHEIPVAFQV
                     GGRIQHRLVNAGQTVAPGDVLFRLDPQELEQTLVATKAMLSAATAELEVAEADVVRYR
                     ALFGKDSISQQAFEREKMAERAAKAQWESAQAQVRHAEIALRHTVLKATAPGILIDVS
                     GNVGQVVAAGQPIALLADLNEWEIEVFLPDGFPPPPTASAHFGNRTVDLKLREVAGAA
                     DATSRTWRVRYSLEKRLDLKLGMLVHVVMQANLDDHPRFTVPLSALDERGDGAHVWKI
                     VEQRAQRFPVSIMDAGPSQARIHGAHLQLGDRIIALGTHLLEPDQSVRARQP"
     gene            4108..4821
                     /locus_tag="VFX11_16955"
     CDS             4108..4821
                     /locus_tag="VFX11_16955"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4940365.1"
                     /translation="MNSKPRTNSERERTDITSDPKSQAATPGRPMDCGKDLAILQAAR
                     ELLFKEGLQAVTMESVARKAGVSKVTVYSRHANRNDLISAVMRQQAEVFSEALTPAQG
                     SKMDVRRSLLAFSRTILDFLVSEDHLQFLRALIAAQGAELDLQGFYGQGPQNILEHLA
                     HGLAAFAAEGQIQCCHPLRSAELFLGMLLRMDLVRALYGVIPHYSEKDLEEHAAFVVT
                     MFLRLHGESSSEEGACDSQ"
     gene            complement(4813..>5028)
                     /locus_tag="VFX11_16960"
     CDS             complement(4813..>5028)
                     /locus_tag="VFX11_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940366.1"
                     /translation="ESGGHILRLASNFYFLRFFQLLRPRPDGTGVFIFRPPSPVQAEW
                     VRGRIPLLNEPEICAAYLSASFVKRSL"
BASE COUNT         1130 a         1505 c         1388 g         1005 t
ORIGIN      
        1 attcccgcta tcttccttcg ccgagaatct gaacgccagt gcgtagagtg ctggcacgaa
       61 caatagggta ataaccgtcc cggcgaccac gccaccaatc agcacgtagg ccatcgggcc
      121 ccagaacaca tccgtcgtca atgggatgaa tgccaacgcc gccgctagag ccgtcaacac
      181 caccggtcgt gcacgccgca ctgccgcttc tatcaccgcg tcccgagggg ttaggccgtc
      241 ggctagattg tcgctgacct gctgggtcag aataagcgtg ttgcgcatca gaatgccggc
      301 caggccgatc agtccgagca aggcgacgaa gccgaagggc ttttggaaca ccagcagcgc
      361 gatcacggcg ccaattacgc ccaagggcgc cgttgccagc acgatcaatg tgccggtgaa
      421 cgtgcgtgtt tgcatcatca cgcaagtgat catcaaggcc agcattaagg gaaacacctg
      481 attgatggaa tcttccgact ccttggaacg ctccaggctg ccgccgatgt caatacgata
      541 cccctccagt aatgcagccc gcagcggctg caaatcgttc cacaccgcct tggtgacatc
      601 ctgcgcctgc gcgcccttga cttcagcctg caccgccagg aacaactggc gctgatggcg
      661 gcgtatcacg gcatcttcgt ataccaattc ggtggtgccc agatgactca aggggatttt
      721 ctgacccgcg ttgttgcgaa cttcgtagat tggcatgtcc agcggcgact gcgtaccgcc
      781 ctgaccgcgc accaacagat ccaccgtcct gatgccttgc cgaacctcgg tcaccgggag
      841 cccttgcaaa cgatactgga tctgctgcgc cacatcccgc ggcgtcatac ccagcaagcg
      901 caagcgttcg tcatccatgt tcagcttcag ggtaggcacg cgctgattcc attctaggtg
      961 cgggtcgacc acatggggat gttgcaacat aacctctcgc accttgccgg cgagttcgcg
     1021 cagggtcgcc gtgtcccgtc ccattacacg gaagctcacc ggccagtcca ccggggggcc
     1081 gaacagcaac tgccggatgc gcacgcgcgc ctcgggaaat gcaccggcct ccacttggtt
     1141 ctctagggca cgcatgatcc tatcgcgttc atgaggacct gcggcagtca cgacgacttt
     1201 ggcgaaagac gaatcgggat gctcgggatt caccgatatg aagaaacgcg gcgagcctcc
     1261 accgatatag ctcgcatagt ttttgacgcc atccatatct ttcaacaggt gctcgatgcg
     1321 cttcaccgtc gcatcggtaa cggcgatgga agtactagga ggtaaggata aatcgatgag
     1381 aatttccggg cggtccgagc cggggaaaaa ctgcttctcc accgcgccag ccatgcccgc
     1441 cacagccaag acgaagaggg tgaccgtagc gagcaccacg gcgccgcgac ggcgcacgca
     1501 aacatccacc accgtgcgaa acctgctgta aaagggggaa tcatacaccc catgatgatc
     1561 cttcttttcc gcatcatggc gcggcttcgc cagcaattgc acgcccagat aaggcgtgaa
     1621 agtgacggca accagccagg aaatcaatag tgaaaacgcc agcacccaaa agatattgcc
     1681 ggcatactct ccgacgccgg atttggcaaa cccgatcggc acaaaaccaa acatcgtgat
     1741 cagagtgcca aacagcatgg gtgatgcggt cgctgtccac gcatgagtgg ccgccgtcat
     1801 cttgctcgcg ccttcttcca gcttcaccag catgatttcg atcgcgatga ttgcatcatc
     1861 caccagcagc cccagagcaa tgatcaaggc gccgagcgtg atgcggtcga gattgatacc
     1921 gctggatttc atcagcacaa aagtcagccc gagggtaatc ggcacggcga tccccacgat
     1981 cagccctgcc cggaagccca gcgccagaca tcccacgccc ataacgacga ccaccgcgac
     2041 gatgaacttt acttgaaaca ggttgactgc cgccgttatc gcgtcggcct ggttggtgat
     2101 ctggtccaag gtcatgccga gcggcaattc cgctttctcc ttctgaagaa atgcggcaag
     2161 tctttcgcca aggtccaggc cattctcgcc cttggccatg accacgccga tgagcaaggc
     2221 atcctggccg ttgacgcgaa ccatatacga tggcggctct tcataaccct gataaaccct
     2281 cgccagatcc cccaggtgca ccactgtagt accggtgcgg atcggcaatg tctccagtcg
     2341 ctgcagtgcg gacagatccg catcaaggcg aaagcgcaag cgtggaccag ccgtgtctat
     2401 ctgacctgag gggatcaggg tgttgtaggc ctggatggtc tcgaacactg tctgcgatga
     2461 aatgccgcta ttggtcatct tgacattatc gaggtcgata aagatgcgct cttctcgctc
     2521 accgatcagc atggctttgc gcacaccgga aatgagctgc aactcatcgc gaagacgctc
     2581 ggcaaagcgg gcgacttcgc gcatgggcag gcctggtgcg ctaaggctgt agaggctgaa
     2641 atagacatca gagaagtcat cattgacaag gggaccaatg acacccttgg gcaaggatgc
     2701 cgcgatatcc agcatgcgct tccttaactg gaactgcagc tccggtaatt tatccatggg
     2761 cgtgtaactg tagaatttga cctgaaggtc ggcccggtcc gccttgacgg tggtgtttat
     2821 ctcctcgacg taatccacct cctggatttt cttttcgatt gggagcacga cttgccgctc
     2881 gatttcagca ggcgtcgccc ccggccatac tgccgacacc actaccatct gcagagtaat
     2941 ggcaggatct tccgcccgcc ccagcgacgc aaacgcgtag gcgcctgcaa gtaccgacag
     3001 aagaatgaaa taaagtgtta ccgaccgctg ctttaccgca aacgaagaaa ggttgggaaa
     3061 catcatggct gacgcgccct caccgattgg tcaggttcaa gcaggtgggt gcccaatgcg
     3121 atgattcgat caccaagctg cagatgcgcg ccatggattc gcgcctgact tgggcccgcg
     3181 tccattatgc tcactgggaa acgctgcgct ctctgttcga cgatcttcca gacgtgagcg
     3241 ccgtcacctc gctcatccag cgcagacagc ggaaccgtga accgggggtg atcgtccaga
     3301 ttggcttgca taacaacgtg tactagcatg cccagcttca ggtcgagacg tttttcgagg
     3361 gagtagcgca ctcgccaagt gcgactggtg gcatctgcgg cgccggcgac ttcgcgtagc
     3421 ttgaggtcga ccgttcggtt accgaagtga gccgaggccg ttggcggagg cgggaaccca
     3481 tccggcagga aaacctcgat ctcccattcg ttcaggtcgg ccagcaacgc gatgggttgc
     3541 ccggccgcca ccacctgccc aacgttgccg ctgacatcaa tcaaaatacc gggggccgtt
     3601 gcttttaata cggtatgccg cagtgcgatt tccgcatggc gaacctgagc ttgcgcggat
     3661 tcccactggg ccttggccgc ccgttccgcc attttctccc gctcgaatgc ctgttgactg
     3721 atgctgtcct taccaaacaa cgcacggtag cggacgacat cggcctccgc tacctccagt
     3781 tctgcagtcg ctgccgataa catggctttt gtggccacaa gcgtttgctc cagctcttgt
     3841 ggatcgagtc tgaatagcac atcgccgggc gccactgtct gccccgcatt gacgagccga
     3901 tgctgaatcc gtccgccgac ctggaatgcc actggaattt cgtggcgcgc acgtaccgtg
     3961 cccgagtacg attgcggtaa gatctgagat tgctctgtga gcgtcactgt gtatacgctc
     4021 ttgactggcg gtgctgtcac cccggaatcg gaaggcgctt tgcaaccgcc gagaagcacc
     4081 aacaaggcga taaagagtat taaacgcatg aacagtaagc cccgcaccaa tagtgaacgc
     4141 gagcgtacag atatcacgtc cgatccaaaa tcacaagccg ctacgcccgg gcgccccatg
     4201 gattgcggca aggatctcgc cattctgcaa gcagcccgag aattgctgtt caaagagggg
     4261 ctacaggccg tcacgatgga atctgtcgcg aggaaagccg gcgtctccaa ggtgaccgtt
     4321 tacagccgac acgccaatcg caacgatctg atctcggcgg tcatgcggca acaagcggaa
     4381 gtgttctcgg aggccttaac cccggcacag ggtagcaaga tggacgttcg gcgctcgttg
     4441 cttgccttta gccgcaccat tctcgacttt ctggtgagcg aggaccatct gcaattcctg
     4501 cgggcgctca ttgccgccca gggggccgag ctggatctgc aaggctttta cggtcaaggc
     4561 ccccaaaata tcctggaaca tctggcgcat gggttggccg cattcgctgc cgaggggcag
     4621 attcaatgct gtcacccctt acgcagcgca gagttgtttc tgggaatgct gctgcgcatg
     4681 gatcttgtcc gcgcccttta cggtgtaata ccgcactatt cagaaaagga tcttgaagaa
     4741 catgcagcat ttgtagtcac aatgttcctg cgactgcatg gggaaagctc gtcggaagaa
     4801 ggggcatgtg attcacaatg acctttttac aaaagaggca gatagatatg cggcacagat
     4861 ttcgggttca ttcaagaggg gtatgcgtcc cctgacccat tctgcttgaa caggtgatgg
     4921 tgggcggaaa ataaaaaccc cggtgccatc aggccggggt cgcaatagtt gaaaaaagcg
     4981 caaaaaataa aagttggaag caagcctcag aatgtggcca ccactttc
//
LOCUS       DASUEG010000900         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1867935, whole genome shotgun sequence.
ACCESSION   DASUEG010000900 DASUEG010000000
VERSION     DASUEG010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867935"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFX11_16965"
     CDS             <1..227
                     /locus_tag="VFX11_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014978872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940367.1"
                     /translation="SEVKAFLAQRESVASLQNEKELNAISKIAQVNQQTRAIAPGDEQ
                     RRLGNPAAEAAGFLRQSGEPARKDDASDKP"
     gene            514..1059
                     /locus_tag="VFX11_16970"
     CDS             514..1059
                     /locus_tag="VFX11_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4940368.1"
                     /translation="MNLFNLKALGCALLLAASSASFAIPMTTVGGVDSLLSSVKLNNS
                     GDATELAWVQSILGSDVTFGGKLEGSEFDWEKVDGTLDVYAQYLTDPSDYFLVKTGKL
                     KGTNDTHFLFRNIADLAYAVIDLSLMGFTWREANIQKVSHISQFDRPTTAVPEPGVLS
                     LLAIGLLGFVLARRMSAGTTH"
     gene            1308..1748
                     /gene="mscL"
                     /locus_tag="VFX11_16975"
     CDS             1308..1748
                     /gene="mscL"
                     /locus_tag="VFX11_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX4940369.1"
                     /translation="MLKEFKQFAMRGNVVDMAVGIIIGGAFGTIVKSLVSDVIMPPIG
                     LLLGGVDFSDLFITLKEGATAGPYATLAAAQSAGAVTLGYGLFINSVVSFMIVAFAVF
                     LLIKGINKLQPPPAATPTTKDCPFCFSSISLKATRCPHCTSELK"
     gene            1745..2395
                     /locus_tag="VFX11_16980"
     CDS             1745..2395
                     /locus_tag="VFX11_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-D-aspartate receptor NMDAR2C subunit"
                     /protein_id="HEX4940370.1"
                     /translation="MIPAGPDVTPAFDPWRQRFLDLLAPWTGAAGASPWYHALRSRYD
                     EPQRHYHTFVHIQACLQHFDAVRPLLQEPVAVELALWFHDAIYDPRAKDNEQRSADFA
                     RTALLACQLDAGLITQVEKLILLTRHDAAPDTDDGRLLVDIDLAILGAAPDLYGRYEQ
                     WVRQEYAHVPHWLYRWGRKRLLRRFLRHPFLYATGHFRQQLEVQARHNLQQAIKNL"
     gene            2510..3520
                     /gene="fbp"
                     /locus_tag="VFX11_16985"
     CDS             2510..3520
                     /gene="fbp"
                     /locus_tag="VFX11_16985"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX4940371.1"
                     /translation="MEIIKTLGEFIIDQQLEYPDATGELTSLFSSIRLAAKILHREVN
                     KAGLADITGAAGADNVQGEQQQKLDVYANERFKNALAQRGVVCGIASEEEEQFVRFEE
                     TRNLDGKYVVLIDPLDGSSNIDVNVSVGTIFSVFRRVSPLGSHVTQEDFLQPGYKQVA
                     AGYVIYGSSTMLVYSTGNGVNGFTYDPTIGVFCLSHPNMKIPADGTIYSINEGNYVHF
                     PDGVKKYIKYCQEEDKSTRRPYSSRYIGSLVSDFHRNLIKGGIYLYPSTSRHPDGKLR
                     LLYECNPMAFLIEQAGGKALAGVGKRILDIQPTELHQRCPLFVGSPNMVDKLEEFIKQ
                     FG"
     gene            complement(3597..4451)
                     /locus_tag="VFX11_16990"
     CDS             complement(3597..4451)
                     /locus_tag="VFX11_16990"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4940372.1"
                     /translation="MSQPAAGASADQAFAEIRKTPLISWPALAIVVVYYAVYIALWKL
                     SLDGAINNVLAAAIGTFVAYLAFTPAHDAMHRAASRVPWVNELILRTATFVAVPFGSG
                     KLFRIMHMQHHRFANDEKDPDHALSSQYRNILLWGVWPFLYLWTWARHRDQYPPIKFY
                     NVWGEAGVGVAIIVAMFWFFPQQMIWLWAVPMYFAFFLMCLVFMVLPHYPHKVKDSDD
                     PYQATVIRTGWEWLLTPLLMYQNYHLVHHLYPTVPFYRYKQAWLAREAFHESHHPAKV
                     TAFQLFRN"
     gene            complement(4653..>5068)
                     /locus_tag="VFX11_16995"
     CDS             complement(4653..>5068)
                     /locus_tag="VFX11_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4940373.1"
                     /translation="RGFLHSHSYTGNPLACAAALATLDIFRDDRVLEQNQTLIRKMQQ
                     ATAHFNDHPHVADVRQTGMILAIELVKNKTTREPYPWQERRGLRVYQHGLDQGALLRP
                     LGSVVYFMPPYVITEEQIDWLAQVAWEGIQKATQD"
BASE COUNT         1055 a         1515 c         1456 g         1042 t
ORIGIN      
        1 tcagtgaagt aaaagcgttc ctggcccaaa gggaatccgt ggcttcgctg cagaacgaaa
       61 aggaattaaa cgccatctcc aaaatagccc aggtgaatca gcagacccgc gccatcgctc
      121 ccggcgatga acagcgacgg ctgggcaatc ctgcggcgga agcagcgggt tttctgcgcc
      181 aaagcggcga acccgcccgg aaagacgacg cgagtgacaa gccctgatgc agtgtcggag
      241 ctcttttcac ctcgctgccg gcaatcctaa ccccagcctg cggatataaa tgcgccaccc
      301 tgacacttca acgggttggc gccgatacct gaattacagc gctgtcggcc atttttacat
      361 tttttcctgc tccagcgagc gcgcattttt ctaagcatac gtctcccaac gttttttttc
      421 cgctggcccg ggccttgctc atgcaacgtg ccctggcagg tctgccaggt ggtcatttga
      481 cattaatcct gcaagatgac ttggggatcc gagatgaatc tgttcaatct aaaagcgctg
      541 ggttgtgcgt tgctgctggc ggccagttcc gcctctttcg ccattcccat gaccaccgtc
      601 gggggagtgg attcgctgct gtcatccgtc aagctgaaca acagtggcga tgcgacggaa
      661 ctggcgtggg ttcaatccat tctgggttcg gacgtcacgt ttggtggcaa gctggaaggt
      721 tctgaattcg attgggaaaa agtggatgga acactggatg tttacgccca gtatctcacc
      781 gatccttcgg attatttcct ggtaaaaacc ggcaaactga aaggcaccaa cgacacccac
      841 ttcctgttcc ggaacatcgc tgacctggcc tacgcggtga tcgatctgtc actgatgggg
      901 ttcacctggc gggaagccaa catccagaag gtgagccaca tctcccagtt cgacaggccc
      961 acaacagcag tccccgaacc gggcgtactg tcgttgctgg ccatcggctt gttgggattt
     1021 gttctggccc gccgcatgag tgccggcacc acgcattgat acgcaaccag gctagctgtg
     1081 caaaagggcg ggatttcccg ccctttttta tgggccggtg ccagcacgct gcgctttccg
     1141 caagtgacgg ccgccttgat ccaggtcggg ataccctgct gtcggccggc atagctcggt
     1201 gatagtatga ctgcggttac ctgggaaaac cgcttgtggc tcaggtacat tggattctta
     1261 atcaatcacg ggcaccaatc acgaggagcg attccatgct ggggaacatg ttgaaggaat
     1321 tcaagcaatt tgccatgcgc ggcaatgtgg tggacatggc ggtcggtatc atcatcggcg
     1381 gcgcgtttgg caccatcgtg aagagcctcg tgtcggatgt catcatgcca cccatcggcc
     1441 tgttgctcgg gggcgtggat ttttccgatc tgttcatcac cctgaaagaa ggcgccactg
     1501 cgggtcccta cgccaccctg gcagcagccc agtcggccgg tgccgtgacg ctgggctacg
     1561 gcctgttcat caattccgtc gtcagcttca tgatcgtggc gttcgccgta ttcctgctaa
     1621 tcaagggcat taacaaattg cagcctccgc cggcagcgac acccaccacc aaggactgtc
     1681 cgttctgttt ttccagcatt tcgctcaagg ccacccgctg ccctcactgc acctctgaac
     1741 tgaagtgatt ccggcagggc ctgacgtcac accggccttc gatccctggc gccagcgttt
     1801 tctcgatctg ctggcgccgt ggacaggcgc cgccggtgcg tcgccctggt accacgcact
     1861 gcggtcgcgc tatgacgagc cgcagcgcca ctatcacacc ttcgtccata tccaggcctg
     1921 cctgcagcat ttcgatgcgg tgcgtccgct gctgcaggag cccgtggcag tggaactggc
     1981 gctctggttt cacgatgcca tctatgatcc ccgcgccaaa gacaacgagc agcgcagcgc
     2041 cgacttcgcc cgcacggctc tgctggcctg ccagctggac gccggcctga tcacgcaggt
     2101 ggaaaagctg attctgctga cccggcatga cgccgccccc gatacggatg acggccgcct
     2161 gctggtggac atcgatctgg cgatcctggg cgcggcaccg gatctgtacg ggcgctacga
     2221 acagtgggtc cgccaggaat atgcccatgt gccgcactgg ctttaccgct ggggccgcaa
     2281 gcgcctgctg cgccggttcc tgcgccatcc cttcctgtat gccaccggac atttccggca
     2341 gcagctggaa gtgcaggcac gacacaacct tcagcaggcc atcaagaatc tgtgagggca
     2401 catatccgct gcaaaccggc acgggtataa tgcgcacgat ccattacctg tatttcatcg
     2461 cacgcaggca ttccctgccg tttcccgtca accgactggt atctcattca tggaaatcat
     2521 caagacgctc ggcgaattca tcattgacca gcaactggaa tacccggatg ccaccgggga
     2581 gctgacctcg ctgttttcct ccatccggct ggccgccaaa atcctgcacc gggaagtgaa
     2641 caaggccggc ctggccgaca tcaccggcgc ggcaggcgcc gacaacgtgc agggcgaaca
     2701 gcagcagaag ctggacgtgt acgccaacga gcgcttcaag aatgcgctgg cgcagcgggg
     2761 cgtggtgtgc gggattgcct ccgaggaaga ggaacagttc gtccgcttcg aggaaacccg
     2821 caatctcgac ggcaaatacg tcgtgctgat cgatcccctg gacggctcct ccaacatcga
     2881 cgtgaacgtt tccgtcggca ccattttcag cgtgtttcgc cgcgtgtcgc cactgggcag
     2941 ccacgtgacg caggaggatt ttctgcagcc gggctacaag caggtggccg ccggatatgt
     3001 gatttacggc tcgtccacca tgctggtgta ttcaaccggc aatggcgtga acggctttac
     3061 ctatgatccc accatcggtg tgttctgcct gtcccacccc aacatgaaaa ttccggcaga
     3121 cggcaccatc tattccatca acgaaggcaa ctatgtgcac ttccccgatg gcgtgaaaaa
     3181 atacatcaaa tactgccagg aagaagacaa gagcacccgg cgcccctatt catcgcgtta
     3241 tatcggatcg ctggtatcgg attttcaccg caacctgatc aagggcggca tttatctgta
     3301 tccgtccacc agccgccacc ccgatggcaa gctgcgtctg ctgtatgaat gcaatccgat
     3361 ggcgtttctg atcgaacagg ccggcggcaa ggcgctggcg ggcgtgggca aacggattct
     3421 ggatatccag ccgacagaac tgcatcagcg ctgcccgctg tttgtcggct cgcccaacat
     3481 ggtggacaag ctggaagagt tcatcaaaca gttcggctga tgaagcagtc tggatgcaat
     3541 aaaaaaggcg ggaattcccc gccttttttg cgcactgact ttttgttaac accggttcaa
     3601 ttgcggaaca actggaacgc cgtcactttt gcgggatgat gcgattcatg aaacgcttcc
     3661 cgcgccagcc acgcctgctt gtagcggtag aacggcacgg tgggatagag gtgatgcacc
     3721 agatgataat tctggtacat cagcagcggc gtcagcagcc actcccaacc ggtacgaatg
     3781 acggtggcct gatatggatc gtcgctgtcc ttcaccttgt gggggtagtg cggcaacacc
     3841 atgaacacca gacacatcag gaaaaacgcg aaatacatcg gcaccgccca cagccagatc
     3901 atctgctgcg ggaaaaacca gaacatcgcg acaatgattg ccacgcccac gcctgcttca
     3961 ccccacacgt tgtagaactt gatcggcgga tattggtccc ggtggcgggc ccaggtccac
     4021 aggtacagga acggccacac gccccacagc aggatgttgc ggtactggct ggacagggcg
     4081 tgatccggat ctttttcgtc gttggcaaaa cggtgatgct gcatgtgcat gatgcggaac
     4141 agcttgccgg agccaaacgg cactgctacg aaagtagccg tgcgcaggat cagttcgttc
     4201 acccagggaa cgcgcgacgc cgcccggtgc atggcatcgt gggcaggcgt aaaggccagg
     4261 taggcaacga aagtgccgat ggccgccgcc agcacgttgt taatggcacc gtccagcgac
     4321 agtttccaca gggcgatata gaccgcgtag tacaccacca caatggccag ggcaggccag
     4381 gatataagcg gggttttacg gatctcggca aatgcctggt cggcgctggc gccggctgcc
     4441 ggttgcgaca tatgaacctc gtgttcccaa tcaataagaa gtcagtgccc cgccacggcg
     4501 agcacttgtg accggcgggt ctgatccacc tacagtagca ggtggattgc ggtcgggtga
     4561 agccggatgg acgggtggcg aggccacttt aatgcgaagt ctaattcgcg ttcaattcgt
     4621 gtttgacacc ggactggcca aacacgactg gctcaatcct gtgtcgcctt ctgaatcccc
     4681 tcccaggcca cctgcgccag ccagtcgatc tgctcttcag tgatcacgta gggcggcatg
     4741 aaatacacca cgctgcccaa cggccgcaac aacgcgcctt gatccagccc gtgctggtag
     4801 acccgcagac cccgacgttc ctgccagggg tagggctcgc gggtggtttt gtttttcacc
     4861 agttcgatgg ccaggatcat gccggtctgg cgcacatcgg ccacgtgggg atggtcattg
     4921 aagtgcgcag tggcctgctg cattttccgg atcagggttt gattctgttc cagcacccgg
     4981 tcgtcgcgga agatatccag ggtggcgagg gcggctgcgc aggctagcgg gttgccggtg
     5041 taggaatgag aatgcaggaa gccccgca
//
LOCUS       DASUEG010000901         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1033881, whole genome shotgun sequence.
ACCESSION   DASUEG010000901 DASUEG010000000
VERSION     DASUEG010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033881"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1072
                     /gene="gap"
                     /locus_tag="VFX11_17000"
     CDS             68..1072
                     /gene="gap"
                     /locus_tag="VFX11_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX4940374.1"
                     /translation="MKKIAINGLGRIGRLVLREYIRNRPANLELVAVNDLVSAENIAY
                     LLKYDSVHGRFEHEVTSGDDHLTIDGVRINFYHQADPHNLPWDTLGVDLVIDCTGVFT
                     HHDKAAFHQQAGAKKVLISAPSETADLTLVVGVNDKAYDTAKHHIVSNASCTTNSLAP
                     PLKVLDAAFGIESVMVTTVHAYTVSQGMVDEPNKKMIRGRAGALNIIPTSTGSDKATE
                     LVLPQLKGKVSALALRVPVPDGAITDIVAVLRRNVTKDEINVALKAAAEGDMDGILGF
                     TTEPLVSTDILGDARSGIVHGLSTRVVNGNHAKVQVWYDNEHGYACRMLDAAVLMCGG
                     "
     gene            1291..2712
                     /locus_tag="VFX11_17005"
     CDS             1291..2712
                     /locus_tag="VFX11_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940375.1"
                     /translation="MKYKNLKGIILFSVLLAGCGGFSAYKWAVDSVFASHGLVVRLDV
                     ADENQFYRIQTDESGVHAATINKSGDIVEEQSFVTEWKNWHKIVSANPGQYFIYSDFA
                     HMYPSEPLHIAFVDVEARVMNENFDLSALLDGYRFRLEGVTVTQKNELVLVGNKYDAT
                     YVNPSKSLIVVVDSAGTVVYTHTDPEVGLFFNATSLKNGDFAVYGSPSLESGANVGRV
                     FRFNPDHQLITQIALVEPDDHTGHVRAITDKGTYFGIHAPNADTVNLKLIDWQENEKF
                     SADVDDEYFFYLFDNGYFHEFDNGSFVLGNRDVIEKFDAMGRSVFRYVPAKLVEGHRV
                     IAVGPHDDLFVGYETKRMSAAPLIATGTDAAGNPTTKIVLSFSGATNVGYQQIATDGT
                     LVRKTQEQPFKFTAEIDQVAWEGTFPSYSATVKSVSPGPCKLAGAVVMPDGMLISHSQ
                     LCDETAVTGGLNSQTPNYTTRAY"
BASE COUNT          709 a          732 c          845 g          689 t
ORIGIN      
        1 cgcaacgatt atcaataatg agccgggcac gacctgcccg ccacgacagt caatcaagga
       61 gtggagcatg aagaagattg cgataaacgg attgggccgc atcggtcgcc tggtgttgcg
      121 cgaatacatc cgcaaccgcc ccgccaatct ggaactggtg gcggtgaacg atctggtgtc
      181 agcggaaaat attgcctacc tgctgaaata cgattccgtg cacggccgct tcgagcatga
      241 agtgacgtct ggcgatgacc atctcaccat cgacggtgtc cgcatcaatt tctatcacca
      301 ggctgatccg cacaacctgc cctgggatac cctgggagtg gatctggtga tcgactgcac
      361 cggcgtgttc acccaccacg acaaggctgc cttccaccag caggccggcg cgaaaaaagt
      421 gctgatcagt gcaccgtcgg aaaccgccga cctgacactg gtggtgggcg tgaatgacaa
      481 ggcctacgac acagcgaagc atcacatcgt gtcgaacgca tcctgcacca ccaactcgct
      541 ggcgccgccg ctgaaagtgc tggatgccgc ctttggcatc gaaagcgtga tggtgaccac
      601 cgtgcatgcc tatacggtgt cgcagggcat ggtggatgag cccaacaaga aaatgattcg
      661 cggccgcgcc ggtgcgctca acatcattcc caccagcacc ggttccgaca aggccactga
      721 actggtgctg ccgcagctga aaggcaaggt gtcggcgctg gcgttgcggg tgccggtgcc
      781 cgatggcgcg attaccgata tcgtggcggt gctgcgacgc aacgtcacca aggacgagat
      841 caacgtcgcg ctgaaagcag cagccgaggg cgacatggat ggcattctgg gtttcaccac
      901 ggaaccgctg gtgtccaccg acattctggg cgatgcgcga tcggggattg tgcacggcct
      961 ttccacccgc gtggtgaatg gcaatcacgc caaggtgcag gtgtggtacg acaatgaaca
     1021 cggctacgca tgccggatgc tggatgcagc ggtgttgatg tgtggcgggt gacatgagaa
     1081 cgccacgtct ggcctgattc cgacactatc cacaagagcc acccgaaagg gtggcttttt
     1141 tttggctaag ggtttgctgc tgatggtgta ctaaaaaatg aattacaggt tgcctcaaac
     1201 aggttaaccc attatctttc ggaaacggaa ttcatattcc gataatggtt gttgcagcga
     1261 tcatcgcaaa ttcatcaagg aatcattttt atgaaatata aaaatctgaa aggaatcata
     1321 ctgttttcgg ttctactggc aggctgtggc ggtttttccg cctataagtg ggcggtggat
     1381 tcagtgtttg cgtcgcatgg actggttgtc cgcctggatg tggcggatga gaaccagttc
     1441 taccggattc agacggacga atctggcgtg cacgctgcca cgattaataa aagcggtgat
     1501 attgttgaag aacagtcatt tgtaacggag tggaaaaatt ggcataaaat tgtgtcggcc
     1561 aatcctggtc agtattttat atattcagac tttgcgcaca tgtatccttc cgagccgctt
     1621 cacattgctt tcgttgatgt ggaagcgcgt gtgatgaatg aaaacttcga tttgagcgcc
     1681 ctgctggacg gttatcgctt tcgtcttgag ggggttaccg tcacgcagaa aaacgaattg
     1741 gttctcgttg gcaacaaata tgatgcgacc tatgtaaatc catctaaaag tctgatcgtc
     1801 gtcgtggata gcgcaggaac ggtcgtatat acgcacactg atccagaagt cggtttgttt
     1861 ttcaatgcga catcgttgaa aaatggcgat ttcgctgttt acggatcgcc ttcgctggag
     1921 tctggcgcca atgttggaag ggtttttcga ttcaacccgg atcaccaact gataactcaa
     1981 attgcccttg tggagcctga tgatcatacc ggtcatgtca gggcgataac cgataaaggc
     2041 acctactttg gcatacatgc acctaacgca gacacggtaa atctcaaatt gatcgattgg
     2101 caggaaaacg agaagttctc cgcggatgtt gatgacgaat actttttcta tctgttcgat
     2161 aacggctatt ttcacgaatt cgacaatggc agttttgtac tggggaatcg ggatgtgatt
     2221 gagaaatttg atgcgatggg tcgcagcgtt ttcaggtacg ttccggcaaa gctggttgaa
     2281 ggccatcggg tcattgctgt tgggccccat gacgaccttt tcgttggata cgaaacgaaa
     2341 aggatgagtg ctgccccgtt aatagcaact gggacggacg cagccggtaa tccgaccaca
     2401 aaaatcgtac tttcatttag tggcgccacc aacgtgggat atcagcagat tgcaacggac
     2461 ggaaccctgg tacgcaaaac acaggagcaa ccgtttaaat tcacggcaga aatagatcaa
     2521 gtggcttggg agggaacgtt tccctcgtat tctgccaccg tcaaatcggt atcgcctggc
     2581 ccgtgtaagt tagccggtgc ggtcgtaatg cccgatggca tgctgatcag ccatagccag
     2641 ctttgcgatg aaaccgcggt cactggtggc ctgaattccc aaacgccgaa ctatacgact
     2701 cgggcctact aggggagttg caggtgcagg tgtaccggca aggcaggcgt tgtcccgtcg
     2761 ggagggacgc ctgcttccag ccaggtcctt caggaacagc cgcatagtgt tgattgctgt
     2821 gctggaaatt gagaagtgga gcgtttactc ttccattccg cgtgcccttg ttcgaggatg
     2881 cgaaatggtt gagaagagcc ttttgcaaca ggtagtgata gaggagcctg cccagcagcg
     2941 cagcaaggac gcccttgacc gctttctgaa ggtgg
//
LOCUS       DASUEG010000902         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2000511, whole genome shotgun sequence.
ACCESSION   DASUEG010000902 DASUEG010000000
VERSION     DASUEG010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..486
                     /locus_tag="VFX11_17010"
                     /pseudo
     CDS             314..486
                     /locus_tag="VFX11_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611820.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(918..2174)
                     /locus_tag="VFX11_17015"
     CDS             complement(918..2174)
                     /locus_tag="VFX11_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940376.1"
                     /translation="MLPALRRLHLFAVLALSGLYLFVSSIALADNDLAYQLKYRLEFL
                     PGSDQALVSITLDNGKLIRSLRFQNQPGQYADIQANGALSVTDTEVRWVPPKAKSTLS
                     LKVKVTRERKPGEFEAMMTKDWALFRGDDIFPAVKVDFVDGAHALATLEVVLPPGWSS
                     IETGWPRIKDNTFRIDNPNRKFDRPTGWMIAGKLGTRRAKVSRTSISVSAPQGSQMRR
                     MDVLTFFKFVWPELHRAFKNTPRKLLIVGMDDPMWRGGLSASNSLFLHADRPIVSENG
                     TSPLLHELTHMMTRIYGAKTAETNDDWIAEGLAEFYSFELIYRAGGMSKERRKNIIKK
                     LAKWGKDVKHLRRQPSTGAFTAQAVVLMDDLDKEIRRRSKGDASIDDVTRDLMAKRTV
                     SLDDLKQSVAALIGPDSETLRSPLLK"
     gene            2370..2942
                     /locus_tag="VFX11_17020"
     CDS             2370..2942
                     /locus_tag="VFX11_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940377.1"
                     /translation="MSFDAEIFEATYIAIGGYAGQKEAVKSAVVENLKTARTEAIKSF
                     MSKQEEMAASDGWLLVKSASVVSTAKTMQSKSVINPSVTGALMVGWHLNKKAYVSYYE
                     LSSGKYISDVKALRKHLDINDDKFTYCAEENIIARYPHVRFLAASAFRVCDNITYPIP
                     ACGYNGKPKTKCKLTLQTLGIKEIEWSTTD"
     gene            2959..3453
                     /locus_tag="VFX11_17025"
     CDS             2959..3453
                     /locus_tag="VFX11_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX4940378.1"
                     /translation="MPDAIDKKLIHEAIVSALKKLVENAQKAMLLAYEAATHEENIAE
                     NKYDTLGLEAAYLTQGQARRLAECEADLAAFQNLVPKTFSTGDAIAIGALVHIEDESG
                     HEQYLLLGPAAGGLKIAAGGQDILVVTPSAPLGQALLHAGVGDTATVHLAGTQKRYEI
                     LAVS"
     gene            3560..4363
                     /locus_tag="VFX11_17030"
     CDS             3560..4363
                     /locus_tag="VFX11_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX4940379.1"
                     /translation="MKTMMASQVEIKPLSIGDPWRWLQQGWQDFKQAPLIGMAHGLGV
                     SAFGLLLLLIAYNQFWLLAGAFTAFLAVAPVVAVGLYAVSQALEKKQPATFLLVFRTW
                     WSWRGQPHHDWRLVRFGLLLCLSGAGWVLTSAAFITLFAPEPVNQPLDFLRHVVLARD
                     TYVFEAWWILGVTLAAPVFASTVITLPLLLDRQIPILDAVVVSWKVVIQNPYPMALWA
                     LLIMVSSMLGILGLAGSVIVVPVLGHASWHAYRDAVGADHLPSRTKGQP"
     gene            4369..4581
                     /locus_tag="VFX11_17035"
     CDS             4369..4581
                     /locus_tag="VFX11_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEX4940380.1"
                     /translation="MLGYTEQDIATFGLTIGLAAFMLYMVFIVAQLARESKAGRFGTF
                     VLFLVLTLGMIGFVAKLVIQWLLGIG"
     gene            complement(4618..5406)
                     /locus_tag="VFX11_17040"
     CDS             complement(4618..5406)
                     /locus_tag="VFX11_17040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940381.1"
                     /translation="MNEALDAKTQDWINRVVYHRKGVERSYTNRILNVAESMALLKYH
                     DYFAGRAVLDIGVGTGRTTRYLYPLASKYVAIDYSPVMIDQMHEAMPEVPAFLCDARD
                     LSMFQDEEFEFIFGSNNVVDALCHEDRLRCLREISRVLKPGGMFLFSSHNRDLAPHLR
                     KPSITWSRNPVDSAIRVRNWLRSMANKARVEQYQREEADYALYCDSGHDYCCIHYYID
                     QRTQRRQLESMGFTVLEVLDPFGQTLQPDDRVPDSHWLMYVATR"
BASE COUNT         1377 a         1677 c         1635 g         1320 t
ORIGIN      
        1 ggaatgtcga atgcagatga cacaacccgt cattcactat cttgaatgct tgcctcttga
       61 agactggaaa atccaatacc cagtgctgtc gcttcgatga ttgatatctg aaaatccgtg
      121 cctccgcaat aaatctgctg gccaggaaca tttcctttgc cgattcagtc aatttctgct
      181 ggttgaccgc attggcacgc cggagtcgaa gccgtccatt tcctgacgtt ggctcctctc
      241 ccccgctgaa aagcaccctc atcacacctt gcgttggcac cctatagagt cccaaacctt
      301 ttcagagtag agcatgcgcg gcgccgatat tacccaggaa tcattgttca ccaccgtcca
      361 gctggaagcc tttgtcctca cgcatcaccc actgcgtggc aaacgggagt tgatgggcac
      421 cgcgctgatc ggctgaacgg gctgtttgac agtgcctacg ctgaactggg tagggcatcc
      481 attgcccgga gtggctgttg cagccgttgc tgctgcagca aagtggacgc caccttccga
      541 ctggtgatga gggcgtggaa tctattgagc acgcacaagt tacgtgagca atgggcccaa
      601 gaaaggttaa aagcgggttg aattcagcga tttcacagaa ggcaaaacac attatctaaa
      661 aaaatatcga aaattcagcc tgagttattg actcaggaag cagcagagag ctgggttccc
      721 gatattttca gcagcctgct ggtctccaca tcaacggcgg gaaccccgtt caattgggcg
      781 tttgcaccgc caccggggtt gaaaagcaca cttgtactcg atgaattgac ggccagaatc
      841 ctggactgcc tgacggaagg ttcccccact gccggcacct ggccgcccca aaacggatgt
      901 ggaaagcaaa tatcacgcta tttgagcaac ggtgagcgca gcgtctcgct gtcaggaccg
      961 atcagcgctg ccacggactg cttcaggtca tccagtgaca ctgtccgctt cgccatcagg
     1021 tcgcgggtca catcgtcgat tgaggcgtcg cccttgctac ggcgccggat ctccttgtcc
     1081 agatcgtcca tcagcaccac cgcctgcgcg gtgaatgcac cggtggacgg ctgacggcgc
     1141 aggtgcttga catccttgcc ccatttcgcc agctttttga tgatattttt gcgccgctcc
     1201 ttgctcatgc cgccggcccg gtaaatcagt tcaaaggaat aaaattctgc gagcccttct
     1261 gcgatccagt catcgttggt ctctgccgtt ttggcgccat agatccgcgt catcatatga
     1321 gtcagttcat gcagcaacgg gctggttcca ttttcgctga cgatagggcg atccgcgtgc
     1381 agaaacagcg aattggacgc agaaagtccg ccacgccaca tgggatcatc catgccaaca
     1441 atcagcaact tcctgggcgt attcttgaag gcacgatgca gctcgggcca gacaaacttg
     1501 aaaaaggtca gcacgtccat ccgacgcatc tggctgcctt ggggagcgct gaccgagatg
     1561 gacgtcctgc tgactttcgc acgtctcgtt cccagcttgc cggcaatcat ccacccggtt
     1621 ggccgatcaa atttgcgatt ggggttgtcg atgcgaaagg tattgtcctt aattcggggc
     1681 cagcctgttt cgatgctgct ccagcccggc ggcagaacca cttccagggt cgccagggca
     1741 tgtgcaccat ccacaaaatc gactttcacg gccggaaaaa tatcgtcacc gcgaaacagc
     1801 gcccagtcct tggtcatcat ggcctcgaac tcacccggtt tgcgctcgcg cgttaccttg
     1861 actttaagcg acaacgtgga ctttgcttta ggtggcaccc agcgcacctc ggtatccgtc
     1921 accgacagtg cgccattggc ctgaatgtct gcatactggc cgggctggtt ctggaagcgc
     1981 agagaccgaa tcagcttgcc attgtccagc gtaatgctga ccagggcctg gtcgctgcca
     2041 ggcagaaatt caagccggta tttgagctga taggccagat cattgtcggc cagtgcaatt
     2101 gaggagacga acagatacag acccgacagg gccagcactg cgaaaaggtg taatcggcgt
     2161 aatgctggga gcatggtcaa cggctgccaa tgacaggctg gaggagtcaa cattctgtgc
     2221 gccgaagcat atcatcaaat atggggagcg gcgacgaatg aaacggcggc caacccgtgc
     2281 aaggcagaat atccgaccaa aacctgcctg caaaacaggc agtcgtcctg ttcgtcacat
     2341 gccaagttca tcacaaaagg agccgtgata tgtcgttcga cgctgaaatc tttgaggcca
     2401 cctatatcgc cattggtggc tatgccggcc agaaggaagc ggtgaaatca gccgtggtgg
     2461 aaaacctgaa aactgcccgc accgaagcca tcaaaagttt catgagcaag caagaggaaa
     2521 tggccgcaag cgatggctgg ctcctcgtca agagcgccag cgtggtcagc actgccaaga
     2581 ccatgcaaag caaaagtgtc atcaatcctt ccgttaccgg tgcgctcatg gtgggctggc
     2641 acctcaacaa aaaggcctat gtgagttact acgagctctc cagcggcaaa tatatcagtg
     2701 acgtaaaagc attgcgcaaa catctggaca tcaatgacga caagtttact tactgcgcag
     2761 aagaaaacat aattgcacgc tatccccacg tgcggtttct ggcagcatcg gcgtttcgcg
     2821 tctgcgacaa cattacgtat cccattccgg cctgcggcta caacggtaaa cccaagacaa
     2881 aatgcaaact gaccctgcaa acactgggca tcaaggaaat cgagtggtct acaaccgact
     2941 gaaggtcaac agcacaccat gcccgacgca attgacaaaa aactgattca tgaggccatc
     3001 gtcagcgccc tgaaaaagct tgttgagaac gctcaaaagg ccatgcttct cgcctatgaa
     3061 gcggcaacgc acgaagaaaa cattgcggaa aacaaatacg atacgctggg ccttgaggca
     3121 gcctatctga cacagggtca ggcccgcagg ctggcagaat gcgaagccga tctggccgct
     3181 tttcagaacc ttgtacccaa gacattttca acaggggatg ccattgcgat tggcgcactg
     3241 gttcacattg aggatgaaag cggccatgaa cagtatctgt tgttggggcc cgctgccggc
     3301 ggattgaaaa tagcggcagg tgggcaggac attctggtcg tgacaccctc tgccccgctg
     3361 ggccaggctc tgttgcatgc aggtgttggc gacacagcaa ctgttcacct ggccggcacg
     3421 cagaaacgtt atgaaatcct ggctgtcagc tgaaggtctg ccagcactca cgctggacgt
     3481 gtcactgcat ggacgctttc ccactccaac atgaatatgc agtagactgc acatcacgct
     3541 catttcggaa ttcccaaaca tgaaaaccat gatggcttcc caggttgaga taaagccttt
     3601 atccattggc gatccctggc gctggctgca acagggctgg caggacttca aacaggcgcc
     3661 gctgatcggg atggcacacg ggctgggtgt ttccgcgttc gggctgctcc tgctgttgat
     3721 cgcctacaac cagttctggc tgctggcagg tgcatttact gcgtttctgg ccgtagcacc
     3781 ggtggtcgcg gtgggtttgt atgcggtgag ccaggcgctg gaaaaaaaac agcccgccac
     3841 gttcctgctg gtgtttcgca cctggtggag ctggcggggc cagccccatc atgactggcg
     3901 gctggtccgg ttcggcttgt tgctgtgcct gtcgggcgcc ggatgggtac tcacgtccgc
     3961 cgcattcatc acgctgtttg cgccggagcc ggtgaatcag ccgctggatt ttctgcgtca
     4021 cgtagtgctg gcgcgtgaca cttacgtatt cgaggcatgg tggatactgg gggttacgct
     4081 ggcggctccg gtatttgcgt ccactgtgat cacactgccg ctgctgctgg atcgccagat
     4141 tcccattctg gatgccgtcg ttgtcagctg gaaagtcgtt attcagaatc cctatccgat
     4201 ggcgctgtgg gcactgctga tcatggtcag ctcgatgctg ggcattctgg gtctggccgg
     4261 cagcgtcatt gtggttccgg tgctgggtca tgccagctgg catgcctatc gggatgccgt
     4321 cggtgccgat catctgccgt cacgaaccaa aggccaaccg tgaacacgtt gctgggctac
     4381 accgagcagg acatcgccac ctttggcctc accatcgggc tggctgcgtt catgctgtac
     4441 atggttttca tcgtggcgca gctggcacgg gaatccaagg ccgggcgatt cggcacattt
     4501 gtgctgttcc tggtgctgac gctgggcatg atcggctttg tggcgaagct ggtcatccag
     4561 tggctgctgg gtatcggatg acgctaacgg ccttccgcat gggcgctgcc gacaggatca
     4621 acgagtggca acgtacataa gccaatggct gtcgggaacc ctgtcgtccg gctgaagcgt
     4681 ctggccaaac ggatcgagca cttccagcac ggtaaatccc atcgactcca gctggcgtcg
     4741 ctgggtacgc tggtcgatgt agtagtgaat gcagcagtaa tcgtgcccgg aatcgcagta
     4801 cagggcgtaa tccgcttcct cgcgctgata ctgctccact ctggctttat tggccatgga
     4861 acgcagccag ttgcggacgc ggatggcgga atcaaccgga ttgcgcgacc aggtaatgct
     4921 gggcttgcgc aggtgcggtg ccaaatcccg gttgtgggag gaaaacagaa acatgccacc
     4981 gggcttgaga acccgggaaa tttcccgcaa acagcgcaag cggtcttcgt ggcacagcgc
     5041 gtcaaccaca ttgttactgc cgaaaatgaa ttcgaactcc tcgtcctgga acatcgacag
     5101 atcgcgcgca tcacacagaa atgccggaac ctctggcatg gcttcatgca tctgatcaat
     5161 catgacagga gaataatcaa tcgcaacgta cttgctcgcc agcggataca ggtagcgggt
     5221 ggtccgcccc gtgccgacgc cgatatccag aaccgcacgc cctgcaaaat agtcgtggta
     5281 tttcagcaat gccatggatt cggcaacatt caggatgcgg ttcgtataag aacgttccac
     5341 tcccttgcgg tgatagacaa ccctattgat ccagtcctga gtcttcgcat ccagtgcttc
     5401 gttcattcgt tcacctgtgc tcgttaaacc gattgcttgt aatctcaacg accgtcgcat
     5461 tcctgttcaa aacagttcag aacacaatat caacaacaaa gacagggtta ataggcaggg
     5521 ccagtccata acgttgtgtc gacgctgatc aggcaaggct gctcgagagc ctcacaccga
     5581 tactaaaggc gaattgccgc cagtcaagtt cactcatcga ataccgcgcc gggcacatac
     5641 ccgtcaccga ccaatcacca gcagcccgtt ttgattccgg ccaaacctcg attgtcgccc
     5701 cgcaaaagtg cagcctttat gacagataca tgacctgctg catgggcaca aatatcctgt
     5761 ggaaacagca gtcaaaaccg atgaaaatac gcgggcctga taaacccgcc atggatggca
     5821 ccggtttggg catacatcgg gccacgcttt tttcccttta tatattgaac atctttttat
     5881 tcagtcattt acgactccgg tcatcggacg ggggaagctg tacatgaaaa ccaagattcc
     5941 ggctttggat gggttgagag gcattgccgt gttatgggtc atgcttcaca acggttcttt
     6001 ttttgcagt
//
LOCUS       DASUEG010000903         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2089369, whole genome shotgun sequence.
ACCESSION   DASUEG010000903 DASUEG010000000
VERSION     DASUEG010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089369"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..860)
                     /locus_tag="VFX11_17045"
     CDS             complement(105..860)
                     /locus_tag="VFX11_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4940382.1"
                     /translation="MHTQSPGPDAQAWSRNLAYLLYQPYKVLIFLPVIVIATNLLGGL
                     IILLVSLGMPRLARPLPVLWARINALAAPARVRVQGSENVDRNQSYVVVANHQSHFDI
                     LAIYGWLGIEIRWVMKIELRKVPVLGLACEKLGHVYIDRSNHRKAQASIDAAKQQLQN
                     GTSIFFFPEGTRSLDGRLLPFKKGAFKLAKELGLPLLPVTINGSHDILPAKTLNLFPG
                     TVDIIIHPPIPVEEASSEEQLMAQARERIASRL"
     gene            1018..1512
                     /locus_tag="VFX11_17050"
     CDS             1018..1512
                     /locus_tag="VFX11_17050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4940383.1"
                     /translation="MVFRPLRACAGLLACWLLPAYAVADCAQLPADARVGNAGCLVVR
                     SGAVLMVQQRLSGGWALPGGTAENGERAACTAARETREETGLTVQVQQHLLTLDNGFN
                     IFRCNYTGEQAPAPQDRMEIRGVAWHDARQRAALSWRFDAQRDQIEALMRQQEAAPST
                     QPTP"
     gene            1522..3006
                     /locus_tag="VFX11_17055"
     CDS             1522..3006
                     /locus_tag="VFX11_17055"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4940384.1"
                     /translation="MDFNNSKAKARSRLRNLLLAVLMSFVLGAVACAWLYFSIQSRDL
                     PEPGFWQDSARLQPALAELDAAAIDSFAGYLRHEQSLFAGLAQLVHEQGRSEPAASRF
                     HPAGPRNPVRFEPNWNRTTENPVRNPLGAVVLVHGLSDSPYSLRTLAELFNQAGYYTL
                     TLRLPGHGTAPGMLLATDLEQFRAAVRVGVQHAVEQVGADKPLLMVGYSNGAPLVVDY
                     SLTQLDGGTDPLPQKLVLLSPALTVNPGAEWAFLRAALGRLPGLGKAGWISVQPELDP
                     FKYASFPAHAGQQLVTLTESIQQRLTRLPDTARQRFPAVLALVSVADATVPAGAVASH
                     LLEQLPASGSELVVFDINRRHEMQWLIKGETLAWLDTLRHRDALPFALSILTNLSDAS
                     SQVQEMRRDAGASASRWQVSEQEGRWPPQVYSLSHIALPFRADDPFYGDDGSQLERLA
                     NLSLRGEKNLFVLPPDQLMRLRYNPFSEYLQQKILVWVNRPVQP"
     gene            complement(3028..4233)
                     /locus_tag="VFX11_17060"
     CDS             complement(3028..4233)
                     /locus_tag="VFX11_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940385.1"
                     /translation="MPTTRKKILCTWELGGDLGHVSRLAGLARELLQRGFEVTVALKD
                     LSRCHGFFADMDINLMQAPVWLPRISMQRPIACLPDTLLLNGYLEPDPLHALVRGWRS
                     LFALVQPDLLLCDYSPTALLASHGSGLPRIQVGTGFSEPATGGPIVDWRPWPATDGLV
                     ARQEQRVLQTINTVLQRLGATPLQRLSDLFATDATFITTYPEFDLYPDRTGATTYCIT
                     RNDQAFGPAVTWPAGNGPRIFAYLKRQHPQLPLLLEALSRCHARVFAACPDGDAALLQ
                     RFTGPNFACSTQLVDLEAAVAEADLFVGHGNMGSVTQSLRAGKPLAALPVHLEQLLTG
                     RQLQQLKLGALVEKIDSADTLAAQLEQWANDPDLHHHAAEFARRHQALAAASVSVLLA
                     DACDAQVTR"
     gene            4443..>4706
                     /locus_tag="VFX11_17065"
     CDS             4443..>4706
                     /locus_tag="VFX11_17065"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4940386.1"
                     /translation="MKHPIQSFPMLALALAVSQATYAQVTAVDDTVTTTVDNTAIINV
                     LANDSSTVGNETMFVDWEGTLQTAYGSVVMGQNNELIYTPNEGY"
BASE COUNT          832 a         1380 c         1575 g          919 t
ORIGIN      
        1 tgtccgcaca tgcgttgatg gtgtggtgtt cgtgagccga aaaaaaagcc gctgatccca
       61 gcggcttttt tgtgcctgtc cggcgagcgg ggtgtggtgg gcgttcacag gcgcgaggcg
      121 atgcgctcgc gggcctgagc catcagctgc tcttcgctgc tggcttcctc caccgggatg
      181 ggcgggtgga tgatgatgtc cactgtgccc ggaaacaggt tcagggtttt cgccggcagg
      241 atgtcgtggg agccgttgat ggtgaccggc agaagcggca ggcccagttc cttcgccagc
      301 ttgaacgccc ctttcttgaa cggcagcagc cggccatcca ggctgcgggt gccttccggg
      361 aaaaagaata tcgaggtgcc gttctgcagt tgctgcttgg cggcgtcgat ggacgcctgc
      421 gccttgcgat gattggagcg gtcaatgtaa acgtggccca gcttttcgca ggccaggccc
      481 agtaccggta ccttgcgcag ttcgattttc atcacccagc ggatctcgat acccagccag
      541 ccataaatag ccaggatgtc aaagtggctc tgatgattgg ccaccaccac gtaggactgg
      601 ttgcggtcca cgttttcact gccttgcacc cgtacccgcg cgggggccgc cagggcattg
      661 atgcgggccc acagtaccgg cagaggccga gccagccggg gcatgcccag cgacaccagc
      721 aggatgatca acccgcccag caagttggtg gcaatcacga ttacgggcag gaaaatcaat
      781 actttgtatg gttgatacag caggtaggcc aggttgcgcg accacgcctg ggcgtcgggt
      841 ccgggcgact gtgtgtgcaa ggggacatcc ctcctgatag ggctcgttgg cgtggtccaa
      901 ttttagggga tgtgactata tgatgacagc gggcacccgg ccgattgttg tttcggaacg
      961 tttccgttgg cgtaatggga aggcaaccgt atctggattt tggaaggtgg tgtggacatg
     1021 gtgtttcgac ccttgagggc ttgtgccggg ctgctggcat gctggctgct gccggcatat
     1081 gccgtggcgg attgcgcgca gttgccggcg gacgcccgcg ttggcaatgc cggctgtctg
     1141 gtggtgcgat ccggggcagt gctgatggtg cagcagcgcc tgagcggtgg ctgggcgctg
     1201 cctggcggta ccgcagaaaa tggcgagcgg gccgcctgta cggccgcgag ggaaacccgg
     1261 gaagaaaccg gtctgactgt tcaggtgcag cagcacctgc taacactaga taacggcttc
     1321 aatatttttc gttgtaacta cactggtgaa caggcgccgg cgccccagga ccggatggag
     1381 atccgtggcg tggcctggca cgatgcccgg cagcgggcgg cgctgtcctg gcgcttcgac
     1441 gcccagcgcg accagatcga ggcgctgatg cgtcagcagg aggcagcgcc ttccacccaa
     1501 ccgactccat aaaaggcgag catggacttc aataacagca aggccaaagc aagatcccgc
     1561 ctgcgtaacc ttctgctggc cgtactgatg agctttgtgc tgggcgccgt tgcctgcgcc
     1621 tggctgtatt tctcgataca aagccgggat ttgccggaac cgggtttctg gcaggactcc
     1681 gcccggctgc agccggcgct ggcggaactg gatgccgcgg ccatcgattc ctttgccggt
     1741 tatctgcgcc acgaacagtc gctgtttgcc ggactggcgc agctggtgca cgagcagggg
     1801 cgctctgaac ccgccgccag ccgcttccat cccgccggcc cgcgcaatcc tgtccgcttc
     1861 gaaccgaact ggaaccgcac gacggaaaat ccggtacgca atccgctggg tgcggtggtg
     1921 ctggtgcatg gtctgtcgga ttccccttat agcctgcgca ccctggcgga actgttcaac
     1981 caggcgggtt attacacgct gaccctgcga ctgcccggcc atggcaccgc gccgggcatg
     2041 ctgctggcca cggacctgga gcagtttcgc gcggccgtac gggtaggtgt gcagcatgcg
     2101 gtggagcagg tgggtgccga caagccgctg ctgatggtgg gctattcgaa cggtgcgccg
     2161 ctggtggtgg attacagcct gacccagctg gacggcggca ccgatccgtt gccacagaaa
     2221 ctggtgttgc tgtcgccggc gctgactgtg aatcccggtg ccgagtgggc gtttctgcgc
     2281 gcggcgctgg gccgtctgcc ggggctggga aaggccggct ggatcagcgt gcagccggaa
     2341 ctggacccct tcaaatacgc ctcctttccc gcccatgccg gccagcagct ggtcacgctg
     2401 accgagtcga ttcagcaacg cctgacccgg ttgccggaca ccgcgcgcca gcgttttccc
     2461 gccgtattgg cgctggtgtc ggtggcggat gccacggtgc cggccggtgc ggtggcaagt
     2521 catctgctgg aacagttgcc ggcctccgga tcggaactgg tggtgttcga catcaaccgc
     2581 cgccatgaaa tgcagtggct gatcaagggg gaaacgctgg cctggctgga taccctgcgt
     2641 catcgtgacg cgctgccgtt tgcgctcagc atcctgacca atctgtcgga cgcgtcatcg
     2701 caggtgcagg aaatgcggcg ggatgccgga gcgtccgcat cccgctggca ggtgagcgag
     2761 caggaaggcc gctggccccc gcaggtgtat tcgctgtcgc acatagcgct gccgtttcgc
     2821 gccgacgatc cgttttacgg tgatgacggc agtcagctgg agcggctggc caatctgtcc
     2881 ctgaggggtg agaaaaacct gttcgtgctg ccgccggacc agctgatgcg gctgcggtac
     2941 aacccgttca gtgagtatct gcagcagaag attctggtct gggtgaaccg gccagtgcag
     3001 ccgtagccgt actggaatgg cgcacggcta gcgtgtcacc tgtgcatcgc aggcatccgc
     3061 cagcaatacg gacactgacg ctgctgccag tgcctgatgc cggcgcgcaa attcagcggc
     3121 atggtggtgc agatccggat cgttggccca ctgttccagt tgcgcagcca gtgtgtctgc
     3181 gctgtcgatt ttttccacca gtgcacccag tttcaactgc tgcagttgcc ggccggtgag
     3241 cagctgttcc aggtgaaccg gcaatgcagc cagcggcttg ccggcgcgta acgactgggt
     3301 gaccgatccc atgttgccgt gccccacgaa caggtccgct tctgcgaccg ccgcctccag
     3361 atccaccagc tgcgtgctgc aggcgaagtt ggggccggtg aagcgctgca gcagtgccgc
     3421 gtcgccgtca ggacaggcgg cgaagacgcg ggcgtggcaa cgggacaagg cctccagcag
     3481 cagtggcaat tgcggatgct ggcgtttcag gtaggcgaaa atgcgcggtc cgtttccggc
     3541 cggccaggtg acagcaggtc cgaaggcctg atcgttgcgg gtaatgcagt aagtggttgc
     3601 accggtccgg tccggataca gatcaaattc cggataggtg gtgatgaagg tggcgtcggt
     3661 ggcgaacaga tcggacaggc gctgcagtgg cgtggcgccc agtcgctgca gcacggtgtt
     3721 gatcgtctgc aaaacgcgct gttcctggcg tgccaccagg ccatccgttg ccggccaggg
     3781 gcgccagtcc acgatggggc ctcctgtcgc cggctcggaa aatcccgtgc ccacctgtat
     3841 gcgcggtaat ccactgccat ggctggccag caaggcggtg ggggaatagt cgcagagcag
     3901 aaggtctggc tgcaccagcg cgaacaggct gcgccatccc cgcaccaggg cgtgcagggg
     3961 atcgggttcc aggtagccgt tcagcaacag ggtgtcgggc aggcaggcga tggggcgctg
     4021 catgctgatc cggggcagcc acaccggcgc ctgcatgaga tttatatcca tatcagcaaa
     4081 aaaaccgtgg cagcgcgaca gatccttcag tgctacggtg acctcaaaac cccgctgcaa
     4141 cagttcccgt gccagcccgg ctaggcgcga aacgtggccc aggtcgccac ccagttccca
     4201 ggtgcagagt attttcttgc gtgtggtggg catgacgttt ccggcaatgg tggtggctgt
     4261 gcgttgcgcc gattctactg caggcaatac acgagcacga tcctggagtg aatacacctg
     4321 cctggtcccg ggtcccgggt cccgggtctt gggtcttggg tcctgggggc gtgtggctgt
     4381 tcagtgagaa tttgaacggt tagtatggtg ggcctctctc ctcgactgac aggattccac
     4441 ggatgaaaca ccctattcag agcttcccca tgctggcact ggcactggcg gtatcacagg
     4501 ccacttatgc ccaggttact gcggtggatg acacggtgac caccaccgtg gacaacaccg
     4561 ccatcatcaa tgtgctggcc aacgattcgt ccaccgttgg taacgagact atgtttgtcg
     4621 actgggaagg cacgctgcaa accgcttacg gcagcgtggt gatggggcag aacaatgagc
     4681 tgatctatac gcccaacgag ggttac
//
LOCUS       DASUEG010000904        11100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1249834, whole genome shotgun sequence.
ACCESSION   DASUEG010000904 DASUEG010000000
VERSION     DASUEG010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249834"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFX11_17070"
     CDS             complement(<1..255)
                     /locus_tag="VFX11_17070"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX4940387.1"
                     /translation="MISTTQDVLRPGQVVIYRSQHQTGSRSEHGLATLRHMAARLAGL
                     KRYEFAGDYDPSQRYDSLYFVPEDTLLHDTARKLGIQDENN"
     gene            complement(398..634)
                     /locus_tag="VFX11_17075"
     CDS             complement(398..634)
                     /locus_tag="VFX11_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HEX4940388.1"
                     /translation="MDSTTHTFADLFIQLGLDGDEVSIRRFCASHTLAGETLLPDADF
                     WTPQQAQFLRESWHQDSDWVAQIDQLNTSLRKHS"
     gene            1319..1846
                     /locus_tag="VFX11_17080"
     CDS             1319..1846
                     /locus_tag="VFX11_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX4940389.1"
                     /translation="MPIASKSNTKAPRKSASRAAVAEHPSRDALSLLKEDHRKVEALF
                     EQYESARKRERKQAIVKTICEELSLHAAVEESVFYPALREVMTHKKDLALLDEAVVEH
                     ATLKGLIAKLESSQPDARFYDATVTVLKEYVNHHVKEEEKEIFAKVRKSALSTMELGQ
                     VMQAAREKLKRKFTH"
     gene            2061..4253
                     /gene="glgB"
                     /locus_tag="VFX11_17085"
     CDS             2061..4253
                     /gene="glgB"
                     /locus_tag="VFX11_17085"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX4940390.1"
                     /translation="MKIQPIVNPAANREWQRVAEGRHHDPFAVLGMHPEGGHWRMTVY
                     RPRADSICLMLNESPVALERVSNSDFFTLAGARSEWGVHPVLLEKRGGDERRYVDPYT
                     FLPQLDEGWLQQFHAGECFTAHEVLGAHPHQSDGIDGILFAVWAPNAERVSVVGDFND
                     WDGRVNPMRTRGSSGVWELFIPGLATGQYYKFELRQRDTGAILLKADPYGRYFEMRPD
                     TSSLIASGDEFRWQDDLWLARRRSADWLHQPMSVYEVHLGSWRRSAGNDYLNYRDIAH
                     QLVPYVKDLGFTHVELMPVTEHPFDGSWGYQVTGYFAPTSRFGSIEDFKYFVDFCHRH
                     GVGVILDWVPAHFPRDTHALASFDGTALYEHADPRRGEHKDWGTLIFNYGRNEVRNFL
                     YSSAYFWLDQFHIDGLRVDAVASMLYLDYSRQPGEWTPNQYGGNENLDAIHFLRRTNE
                     VVHQAFPGVLMMAEESTAWPQVSRPVYLGGLGFSMKWNMGWMNDTLAYFLQDPLFRQY
                     HHDKLTFSMMYAYSENFMLPLSHDEVVHGKRSLLEKMPGDAWQQFAGLRLLFTYLYTH
                     PGKKLLFMGSEFGQGREWDHDHGLDWHLLQHDFQAGAQRAVRDLNLLYCRQPALHQWE
                     FEPRGFEWIDCHDHAQSVISYVRRSENGFLVVVLNFTPVVREHYRIGVPEAGVYGECF
                     NSDSVFYAGSNVSNGVAIQSEAVPCMARANSILLTLPPLGGIVLQKAL"
     gene            4320..5261
                     /locus_tag="VFX11_17090"
     CDS             4320..5261
                     /locus_tag="VFX11_17090"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4940391.1"
                     /translation="MTSSTYPHNRRTGINARTVSIPPDKQTAFRALTQTPRVAWPTLA
                     LAAVCLSGALFCMVAGFTGRLNIYLACLLIAFNFYWFFSVIHDAIHRSVSRYRWLNET
                     VGQLASSLFNPIASLELFRWGHLQHHRHTNDEQDIDYWCHGPAWSLPLRWMLIDGQYL
                     LHALRSKEASAKKALLKALPFMLGGAVLIVWLIASGHGFEYLMLSLVPSRIAFIFIGF
                     SFFWLPHAHWPDQYQDLRQSQNLTIASTVRTGYEWLLNPLLQYQNYHLIHHVWPGTPF
                     YNNTRLWQLLEPEFRQRDLAVTPGFRLLPELHRVSEH"
     gene            5303..6205
                     /locus_tag="VFX11_17095"
     CDS             5303..6205
                     /locus_tag="VFX11_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX4940392.1"
                     /translation="MATRYFLLDVFADVPFQGTQIPVVVPERRLSDSEKLLIASEFQK
                     SETVFLDTANAEAPFSVYTDTRQTIFGAHTILAAAYIAHEMGLSQDQGAYTSFEIVQN
                     RQPIKVFIDKVPDGIGSIQFARTIVPSIDRFTPSLSRIANALGIDEKHISFSKYRPMV
                     VSVDNPTLIIPFTRAEHVQAAKLNSESWSELLSETYAADIFLFAPKSHKDIEDFHGRI
                     VNPNVASNEFPPIGNVMPEFVAFLAEQKDTADGTHTFSIDRGSEKTRKSVLHVEFDKR
                     PGKSIQCRIGGRVVKMGVGTLLFP"
     gene            complement(6274..7938)
                     /gene="pgi"
                     /locus_tag="VFX11_17100"
     CDS             complement(6274..7938)
                     /gene="pgi"
                     /locus_tag="VFX11_17100"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4940393.1"
                     /translation="MPNPVNAPVWKRLQELQQQLAGQRLQTLFEQDSQRFTQFSAQVG
                     NIFIDYSKNFIDGETLDALLELAGDQQLKDATNRLASGQQIAPTVNAVPYTALRDRSF
                     LPVCIDGEDVSSGVRNVLFTMRRLCENIRSWNWRGYNGKPLTDIVTLGVGGSGNGAEM
                     VCRALAPYHSPSLKSHFIANADAQAIRDKLAQLHPDTTLFIISSKSFVTQETLQNARL
                     AKAWVTANASTADAMHQHFIAITSNKAAALEFGIPEDNILPVWDWLPGRYSLWTSAGL
                     PIALAIGMERFEQMLDGAHLIDQHFLNAPLRQNLPALMGLLGVWYANFWNTPSQAILP
                     YSELLDLLPGYLQHSLMETNGKSIDLEGQPVNYSTAPVIWGSAGTKNQHTYFQLLHQG
                     THLVPADFIAVASDTSGSDDSVTTFLHLLAQTETLMRGTTPEQDVGSTSDEQRLPGNK
                     PSTSLLLADLSPGTLGQLLALYEHEGFVQSVLWNLNPFSHWGTELGKRYCAGLGSASI
                     DEIAAARHDSSTLGLLGVFKRMQEEQHRRDPFHTGNMREESVYTVF"
     gene            8182..9618
                     /gene="glgA"
                     /locus_tag="VFX11_17105"
     CDS             8182..9618
                     /gene="glgA"
                     /locus_tag="VFX11_17105"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX4940394.1"
                     /translation="MNLLFATSELFPLIKTGGLADVSQSLPLALRQAGVDVTLVLPAY
                     RSVLAQLPEPLARWPVNDAFVPAETVLLENRLPGSDLRLLLVQPGDLFDREGGPYSDA
                     KGLDWPDNADRFAQFCRVVVALLAGRCGAEPFSAGHFDVVHCNDWQTGLVPALLALEP
                     VPVRSIFTIHNLAYQGLFSRAVFERLALPEAWWHHDRLEFYGNFSFLKAGIVYADAIT
                     TVSPTYAREILEEPLACGMGGLLQYHRHKLQGILNGADYSLWNPATDSHLRFHYDVDS
                     LPEKLHNKLDLQRSLGLRLGKSIPLLGVVSRLVYQKGADWIIDCMQRLQGEKLQWVVL
                     GTGDRQHEQQLLALARAVPKQIAVTIGYDEAMAHRIEAAADLFVMPSRYEPCGLNQIY
                     SLRYGTLPLVRATGGLADTVTDCTDGFAYRGDTASDLEQCIRRAIGYFSKSKTWEKMQ
                     RNAMGQRFDWQQSAQAYLALYQHRDSRN"
     gene            9621..10616
                     /locus_tag="VFX11_17110"
     CDS             9621..10616
                     /locus_tag="VFX11_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX4940395.1"
                     /translation="MHYDIVAIGSATVDQFADTDSELIRIETPTMQEKLIAFPLGSKI
                     LIRKLELATGGGGTNCAVALARLGLKTAYLGKIGDDGNGEYVVRQLAGEQVDFIGPRE
                     GNTGTSIILNSFTHDRTILAYKGANNNLRPDEIKPFDTHWVYLASMLETSFDTVVDLI
                     CRHDFRVAFNPSNYQAELGYKALAKLIDRVEILVMNLEEACKFLGLDYRKPPAATTLL
                     QEMAKLPPRIFVITDGSEGVHVYDREFYYRGLPAPDLQVVEATGAGDAFASTFTAACV
                     LGEPVERAIHLAMTNAESVLKYRGAKEKLLTRAELTETAQQVQRSISKMPLPAAR"
     gene            10656..>11100
                     /locus_tag="VFX11_17115"
     CDS             10656..>11100
                     /locus_tag="VFX11_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940396.1"
                     /translation="MQRSAVITFQIDQLIPNAGENESGWIVSVWEGANGFLQNLEFSC
                     EDPYRSALAEYRISARLDAAVKLLQQARSKAGSFAGYLQQSQQGSSLSTGAAITIEIQ
                     AAKGNLELYSMYNAVGTFIHQLVLAFNLAFPGSCRFVGTHYEGPDA"
BASE COUNT         2229 a         3161 c         3332 g         2378 t
ORIGIN      
        1 attgttctca tcctgaatac ccagcttgcg tgcagtatcg tgcagcaacg tatcctcggg
       61 aacaaaatat aggctgtcgt agcgttgcga cgggtcatag tcgccggcaa attcatagcg
      121 cttgagtcct gcaaggcggg cagccatgtg gcgcaatgtc gctaatccgt gttcagaacg
      181 gctgcctgtc tggtgctggc tacggtagat tacgacctgt ccggggcgta gcacatcttg
      241 cgttgtcgaa atcatcagaa tgcatccgta gttgggacga cgtcatggtt gcgatccgct
      301 ggcgatatgg ccgcagcccg gatggcgtct tgtggctgta aagctcattc ctgccgctac
      361 cttgcgtcgt cacgctgttg tcaaaaatgc gcaggcatca gctgtgtttt ctcaggctgg
      421 tattcagctg gtcaatctgc gctacccagt ccgagtcctg gtgccagctt tcacgcagaa
      481 attgagcctg ctgcggtgtc cagaaatctg cgtccggaag cagcgtttcg ccagcgagcg
      541 tgtggctggc acagaagcga cggattgaga cttcatcgcc gtccagaccg agttgtatga
      601 agagatctgc aaaagtgtgt gtcgtgctgt ccatcctgtg gctccgaaaa ttatgaatgg
      661 ttttcctgat agtccttcac ggattttctg tgggcggcat ggcgacggac tctgcttcag
      721 gcgattcgca ccgaaagagg aatagcatca gcatcaccgt tgtgcctgtc ggtaaggcag
      781 cacgtcttca gtctcttgcc ggctatagac gatgtggccc gtcaggctcc cgccaaacgc
      841 cgtcacaaat tccgtcacag aacagcgggc aaagagaaag atcgacaatc cattaccctt
      901 tagcgatttg ccatggcttc ctgggtgcat ccatcccctg gccatactgt gtgaccctgc
      961 atttttcact gtcagcggtt ctgcggtcga tgacgaaaat tttccaagca atgtgcatgc
     1021 ctgctttgtg gaatgcagaa gcggcagggc tggcggtcgt ggcggcatgt cgataattgt
     1081 gtgagttacc agaagatttg caattcttgc taaatccaga aggttaagaa tacgggcagg
     1141 ctgcgttatg cagggttacg cacgctgcat cagcgtcagg ctgccgcttc cggggctgct
     1201 gggtgttaaa aaatcaggtt tgcattgcag gatggcatgc agcttgcctc gtatatcgcg
     1261 caggcagcgg attgcagttc gctgtgtcac cgggtaaaca caggacagga gattcctaat
     1321 gccgattgca tcgaagtcaa ataccaaagc accccgcaaa tccgcgtcgc gcgcagcggt
     1381 ggcggaacat ccgagtcggg acgcactttc gctgctgaaa gaagaccacc gtaaagttga
     1441 agccttgttt gaacaatacg aatcggcgcg caagcgagag cgcaagcagg ccatcgttaa
     1501 aaccatctgt gaggagttgt cattgcacgc ggcagttgag gaatccgtct tttacccggc
     1561 cctgcgtgaa gtgatgaccc acaagaagga tctggcgctg ctggacgaag ccgtggtgga
     1621 gcatgccacg ttgaaagggt tgatagccaa gctggaatct tcccagccgg acgcccgctt
     1681 ttacgatgcc accgttaccg tgctcaagga atacgtgaac caccatgtca aggaagagga
     1741 aaaagagatc tttgccaagg ttcgcaaaag cgcactgtcc acgatggaac tgggtcaggt
     1801 gatgcaggcc gcccgcgaga agctgaagcg gaagttcacg cactgaagtt ggtcccgccc
     1861 tgcaatccag gcggatgcct gtgctggccg ggtaaccgtc agcaagcggc agcctgatga
     1921 tttccggctt cgaaaggcca tggcttcccg gcgcccggag aggtatcctt catgtagaag
     1981 ggctcgcggg acggagcaaa ccacgcgtgg cgtggttgat cagaacccgt gtgtaatcag
     2041 aaccagtgtc agagaatcta atgaaaattc agcctatagt gaatccggcg gcgaaccggg
     2101 aatggcaacg ggttgcagaa ggacgtcatc atgatccgtt cgccgtgctg ggcatgcatc
     2161 ctgaaggtgg ccactggcgc atgacggtgt atcggccccg ggcggattct atttgtctaa
     2221 tgctgaatga atcgccagtg gcactggagc gcgtgagcaa ttcggatttc tttacgctgg
     2281 ccggcgctcg cagcgaatgg ggggtgcacc cggtactgct ggaaaaacgc ggtggcgacg
     2341 aacgacgcta tgttgatccc tacacgttcc tgccgcagct ggacgagggc tggctgcaac
     2401 agtttcatgc tggtgagtgc ttcaccgccc acgaggtgct gggcgctcat ccgcatcagt
     2461 ctgatggtat cgacggtatt ctgtttgccg tgtgggcacc caatgcagaa cgggtcagtg
     2521 tcgtggggga tttcaacgac tgggacggcc gcgtgaatcc catgcgcacc cgtggcagtt
     2581 ccggcgtgtg ggaactgttc ataccggggc tggcgacagg gcaatactac aagttcgaac
     2641 tgcggcagcg cgacaccggc gcgattttgc tgaaagctga tccctatggc cgctatttcg
     2701 aaatgcgacc cgacacttca tcgctgattg ccagtggcga tgagttccgc tggcaggacg
     2761 atctgtggct ggcgcgccgg cgcagtgccg actggctgca ccagcccatg tcggtttatg
     2821 aagtgcatct gggttcctgg cgtcgcagcg cgggcaacga ttatctcaat taccgtgaca
     2881 tcgcccacca gctggtcccc tatgtaaagg acctgggttt tacccatgtg gaactgatgc
     2941 cggttaccga gcatcccttc gacggttcct ggggttacca ggttactggc tactttgctc
     3001 ccaccagccg ctttggcagc atcgaggatt tcaagtattt cgtggatttc tgccaccgcc
     3061 atggcgtagg cgtgattctg gactgggtgc cggcacattt cccgcgcgac acccacgccc
     3121 tggccagttt tgatggtacc gcactgtatg aacatgctga tcctcgccgg ggagaacaca
     3181 aggactgggg cacactgatc ttcaactacg gccgcaatga agtgcgcaat tttctgtatt
     3241 ccagcgcgta tttctggctc gaccagtttc acatcgacgg cctgcgcgtg gatgcggtgg
     3301 cgtcaatgct gtatctggat tattcgcgcc agccgggcga gtggacaccg aaccagtacg
     3361 gcggcaatga aaatctggat gccatccatt ttctgcgccg caccaacgaa gtggtgcacc
     3421 aggcctttcc cggcgtgctg atgatggcag aggaatccac tgcctggccc caggtgtcgc
     3481 ggccggtcta tctgggtggt ctgggtttca gcatgaaatg gaacatgggc tggatgaacg
     3541 acacccttgc ctatttcctg caggacccgc tgttccgcca gtaccaccat gacaaactga
     3601 ccttcagcat gatgtacgcc tactcggaaa atttcatgct gccgctgtcc cacgatgagg
     3661 tcgttcacgg caagcgttct ctgctggaga agatgccggg cgatgcctgg cagcagtttg
     3721 ccggactgcg gttgctgttc acgtatctgt acacgcaccc cggtaaaaaa ctgctgttca
     3781 tgggcagcga gttcggccag gggcgcgagt gggatcacga tcacgggctg gactggcatc
     3841 tgctgcagca tgattttcag gccggcgcgc agcgcgcggt acgggatctt aatctgctct
     3901 attgccggca accggcactg caccagtggg agttcgaacc gcgcggcttt gagtggatcg
     3961 actgtcacga tcatgcccag tcggtgatca gctatgtgcg tcggtcggag aatggctttc
     4021 tggtggtggt gctgaatttt acgccggtgg tccgggagca ttatcgcatc ggcgtgccgg
     4081 aggcgggcgt gtacggcgaa tgcttcaatt cggattcagt gttttatgcc ggcagtaatg
     4141 tgtcgaatgg cgttgccatc cagtccgaag cggtgccctg tatggcacgg gctaacagta
     4201 tcctgctgac actgccaccg ctgggcggaa tcgtgttgca gaaagccctg tgaacgcttg
     4261 catcctggac aatgccgttg tggaatgaac tttccagcgc gcaccgggga acctgtaaca
     4321 tgaccagctc cacctatcca cacaaccgca gaacaggcat caatgcccgc accgtttcca
     4381 ttcccccaga caagcagact gcatttcgcg ctttgacgca aacgccccgg gtagcgtggc
     4441 ccacgctggc cctggcagcg gtctgtctga gcggcgccct gttctgcatg gtggccggct
     4501 tcaccggacg cctcaacatc tatctggcat gcctgctcat tgcgtttaat ttctactggt
     4561 ttttcagcgt catacacgat gcgatccacc gtagtgtcag tcggtaccga tggctgaacg
     4621 aaaccgtggg ccagttggcc tccagcctgt tcaatcccat cgcatccctg gaactgttcc
     4681 gctggggcca tctacagcat caccgccaca ccaatgatga acaggacatc gattactggt
     4741 gccacggccc cgcctggtcg ctgccactgc gctggatgct gatcgacggg cagtacctgc
     4801 tgcatgcact gcgcagcaag gaagcgtcag caaagaaggc cctgctgaag gcgctgccgt
     4861 tcatgctggg tggtgccgtc ctgattgtct ggctgatcgc cagcggccac gggtttgaat
     4921 atctgatgct gagtctggtg ccgtcccgca tcgcatttat ctttatcggt ttttcctttt
     4981 tctggctacc ccatgcccac tggcccgatc agtaccagga tttacgccaa tcgcaaaatc
     5041 tcaccattgc cagcaccgtg cgcacagggt atgaatggtt gctgaatccg cttttgcagt
     5101 accagaacta ccatttgatc caccatgtct ggcccggtac tccgttttac aacaataccc
     5161 ggctttggca attgctggag ccggaattcc gccagcggga tctggcggta actcccggct
     5221 tcaggctgct gccggaattg caccgggtca gcgaacactg atcgactttt cggacaatca
     5281 taaacaggac aaggaatcct ttatggccac ccggtacttt cttttggacg tttttgcaga
     5341 tgtgcctttt caggggacgc agatacccgt tgtggtaccg gagcggcgac tttccgacag
     5401 cgaaaagctg ttgatagcga gcgaatttca gaagtccgaa accgtgtttc tggacacggc
     5461 gaatgccgag gccccgttca gtgtctacac cgacacccgg cagaccatct ttggcgcgca
     5521 caccattctg gcggccgcct atatcgccca tgagatgggg ctgagccagg atcagggtgc
     5581 ctatacgtcg tttgaaatcg tgcagaaccg gcagccgatc aaggtgttta tcgacaaggt
     5641 accggacggc atcggttcaa ttcagtttgc gcggacgatt gtgccgtcga tcgatcgctt
     5701 cacgccatcg ctgagccgca ttgcaaatgc gctgggcatc gatgaaaaac atatttcatt
     5761 cagcaaatac cgtccgatgg tggtcagtgt cgacaacccg acgctgatca ttccattcac
     5821 ccgggccgag catgtgcagg cggcgaagct caatagtgaa tcctggtccg agctgctgtc
     5881 ggagacctac gctgccgaca tattcctgtt cgcgccgaaa tcgcacaagg acatcgagga
     5941 ttttcatggc cgcatcgtca atccgaatgt ggccagcaac gagtttccgc ccatcgggaa
     6001 cgtgatgccc gaattcgttg cctttctggc cgaacagaaa gataccgcgg acggtaccca
     6061 tactttcagc attgatcgcg gcagcgagaa aacgcgcaaa agcgtgctgc atgtggaatt
     6121 cgacaaacgt cctggcaaaa gcatccagtg ccggatcggc gggcgggtgg taaaaatggg
     6181 cgtggggacg ttgctgtttc cgtaactggc gtgaaaagcc ggggcgaact gacaattcca
     6241 gcgcctgaac cggctgatcg gttctgccgg cgttcagaaa accgtgtaaa cgctttcttc
     6301 ccgcatgttg ccggtgtgga acggatcacg ccggtgctgt tcttcctgca tgcgtttgaa
     6361 tacgcccagc aatcccagcg tggaactgtc gtggcgggcg gcagcgatct cgtcgatgct
     6421 ggcgctgccc agcccggcgc aataccgctt gcccagctcg gtgccccagt gcgagaacgg
     6481 attcaggttc cacagcacgc tctgtacaaa tccttcatgt tcatacagtg ccagtaactg
     6541 gcccagtgtg ccgggcgaca gatccgccag cagcaggctt gtggacggct tgttgccggg
     6601 caaccgctgt tcgtcactgg tgcttcccac atcctgttcc ggggtggtgc cacgcatcag
     6661 ggtctcggtc tgtgccagta aatgcaggaa cgtggtgacg gaatcgtcgc tgccgctggt
     6721 atcggacgcc actgcaatga aatctgccgg taccaggtgc gtgccctggt gcagcagctg
     6781 gaagtaagtg tgctggttct tggttccggc ggaaccccag atcaccggtg cggtggagta
     6841 attcaccggc tggccttcca gatcgatcga cttgccgttg gtttccatca gactgtgttg
     6901 cagataaccc ggcaacaggt ccagtaattc gctgtagggc aggatcgcct gcgatggcgt
     6961 gttccagaaa ttcgcgtacc agacgcccag caatcccatc agcgccggca gattctggcg
     7021 caaaggcgca ttcaggaaat gctggtcgat caggtgggcg ccgtccagca tctgctcgaa
     7081 ccgctccatg ccgatggcca gcgcgatggg caggccggca ctggtccaca gggagtagcg
     7141 gcccggcagc cagtcccaca ccggcaggat gttgtcttcc ggaatcccga attccagcgc
     7201 tgccgccttg ttggaggtga tggcgatgaa atgctggtgc attgcatccg cggtgctggc
     7261 attggcggtg acccaggctt tggccaggcg ggcattctgc agggtttcct gggtgacgaa
     7321 actcttgctg gaaatgatga acagcgtggt gtcgggatgc agttgagcca gcttgtcgcg
     7381 gatcgcctgg gcatccgcat tggcgatgaa atgggatttc agcgacggcg aatggtaggg
     7441 cgccagtgcc cggcacacca tctcggcgcc gttgcccgat ccgcccacgc cgagtgtgac
     7501 gatgtcggtg aggggcttgc cgttgtagcc gcgccagttc caggaacgga tgttctcaca
     7561 caggcggcgc atggtgaaca gcacgttgcg cacaccggat gacacgtcct cgccgtcgat
     7621 gcacacgggc aggaaggagc gatcccgcag tgcagtgtac ggcacggcgt tcaccgttgg
     7681 cgcgatctgt tgtccgctgg cgagccggtt ggtggcgtcc ttgagttgct gatcgccggc
     7741 cagttccagc agcgcatcca gggtttcgcc gtcgatgaaa ttcttggagt agtcgatgaa
     7801 aatattgccg acctgcgccg agaattgcgt gaagcgctga ctgtcctgtt cgaacagcgt
     7861 ctgcagtcgc tgaccggcaa gctgttgctg cagttcctgc aggcgtttcc aaacgggggc
     7921 gttgacaggg ttcggcatga cggactccat ggcaatctgg cgggtggacc ctgcgtgcgt
     7981 gatcctgcgc gcgctgatga tagggctttg attgccagct tagagccaga tcatgggtgc
     8041 caatttgacc gccgtcaaat cccggcggtg cacgggtgtg ctgctcctgc ttccctgcca
     8101 aagcgcctat gctaaagtta cgccagtttt cccagtcatt acgccacctt atcattcacc
     8161 acacgggaaa ccggatacgc gatgaatctg ctctttgcta ccagcgaact tttccccctg
     8221 atcaaaaccg gcggactcgc ggacgtgtcg cagtcactgc cgctggccct gcgccaggcg
     8281 ggtgtggacg tcaccctggt actgccggcc taccgttcag tgctggcgca gctgccggaa
     8341 cccctggccc ggtggccggt gaacgatgcc ttcgtgccgg cggaaacggt tctgctggaa
     8401 aatcgcctgc caggcagcga tctgcgcctg ctgctggtgc agccgggtga tctgttcgat
     8461 cgcgagggtg gtccctacag cgatgccaag ggcctggact ggcccgacaa tgccgatcgt
     8521 ttcgcccagt tctgccgggt ggtggtggcg ctgctggcgg gccgttgcgg cgcggagccg
     8581 ttcagtgccg gccattttga tgtggtgcac tgcaacgact ggcagacggg gctggttccc
     8641 gcgctgctgg cgctggaacc ggtgccggtg cgcagcatct tcaccatcca caatctggcg
     8701 tatcaggggc tgttttcccg tgctgtgttc gaaaggctgg ccctgccgga agcctggtgg
     8761 catcacgatc ggctggagtt ttacggcaat ttctcgttcc tgaaagcggg catcgtgtat
     8821 gccgacgcca tcaccaccgt cagccccacc tatgcgcgcg aaatcctgga agaaccactg
     8881 gcctgcggca tgggcgggct gctgcaatat caccgtcaca aactgcaggg tattctgaat
     8941 ggcgcggatt acagtttgtg gaatcccgcc actgattccc acctgcgttt tcactacgac
     9001 gtcgacagcc tgccggaaaa acttcacaac aaactcgatc tgcagcgcag cctggggttg
     9061 cggctgggca aatcgatccc gttgctgggt gtggtgagcc ggctggtgta ccagaagggt
     9121 gccgactgga tcatcgactg catgcagagg ctgcaaggcg aaaaactgca atgggtggtg
     9181 ctgggcaccg gcgaccgcca gcacgagcag caattactgg cactggcgcg ggccgtgccg
     9241 aagcagatcg ccgtgaccat cggttatgac gaagccatgg cccaccgcat cgaggccgcc
     9301 gccgatctgt ttgtcatgcc atcgcgctac gagccctgtg gcctgaacca gatctacagt
     9361 ctgcgttacg gcaccctgcc actggtgcgt gccaccggcg gcctggcgga cacggtgacg
     9421 gactgcaccg acggtttcgc ctaccggggc gatacggctt ctgatctgga acaatgtatc
     9481 cgccgggcga tcggttactt tagcaagagc aagacgtggg aaaaaatgca gcgcaatgcc
     9541 atgggccagc ggtttgactg gcagcagagc gcgcaagcct atcttgccct ttaccagcac
     9601 cgggacagca ggaattgaag atgcattacg acatcgtggc catcggcagc gccaccgtgg
     9661 accagttcgc cgacaccgat tccgaactga tccgcatcga aacgcccacc atgcaggaga
     9721 agctgattgc gtttcccctg ggcagcaaga tcctgatccg caaattggag ctggccactg
     9781 gcggcggcgg caccaactgc gcagtggcgc tggcacggct gggcctgaaa accgcatacc
     9841 tgggcaagat cggcgacgac ggcaacggcg agtacgtggt gcgccagctg gccggcgaac
     9901 aggtcgattt catcggcccg cgggaaggca acaccggcac ctccatcatc ctcaacagtt
     9961 tcactcacga ccgcaccatc ctggcctaca agggcgcgaa caacaatctg cgtccggacg
    10021 agatcaaacc gttcgacacg cattgggttt atctggcgag catgctggaa accagttttg
    10081 acacggtggt ggacctgatc tgccgccatg atttccgcgt ggcattcaat cccagcaatt
    10141 accaggcgga gctgggatat aaagcgctgg cgaaactgat cgaccgggtg gaaatcctgg
    10201 tgatgaacct ggaggaagcc tgcaagttcc tggggctgga ttaccgcaag ccgccagcgg
    10261 ccaccacgct gctgcaggaa atggcgaaac tgccgccgcg catctttgtg atcaccgacg
    10321 gcagcgaagg cgtgcatgtt tacgatcggg aattctatta tcgtggcctg cccgcgccgg
    10381 atctgcaggt ggtggaagcc acgggcgccg gcgatgcctt tgcttccacc tttaccgctg
    10441 cctgcgtgct gggtgaaccg gtggagcgtg ccattcacct ggccatgacc aatgccgaat
    10501 ctgtgctgaa ataccgtggc gcgaaggaaa agctgctgac ccgcgccgag ctgactgaaa
    10561 cagcacagca ggttcagcgc agcatcagca aaatgccgct gcctgctgca cgctgacagc
    10621 tttgattgca ccttgcctgt ccggagacca tcgccatgca acgttcagcc gtcatcacgt
    10681 tccagattga tcaactgatc cccaatgccg gggaaaatga aagcggctgg atcgtgtcag
    10741 tctgggaagg cgccaatggc tttctgcaga atctggaatt cagctgcgaa gatccctatc
    10801 gttctgcgct ggcggagtat cgcatcagcg cgcgcctgga tgcggcggtg aagttgttgc
    10861 agcaggcccg cagtaaggca ggcagcttcg cgggttacct gcagcagtcc cagcagggca
    10921 gcagcctgtc caccggcgcc gcgatcacca tcgagatcca ggcggcaaaa ggcaatcttg
    10981 aactctattc aatgtacaac gctgtgggca ccttcattca ccagctggtg ctggcgttca
    11041 atctggcgtt ccccggttcc tgccggttcg tgggcaccca ttatgaagga cccgacgctg
//
LOCUS       DASUEG010000905         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_477004, whole genome shotgun sequence.
ACCESSION   DASUEG010000905 DASUEG010000000
VERSION     DASUEG010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477004"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFX11_17120"
     CDS             <1..641
                     /locus_tag="VFX11_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4940397.1"
                     /translation="GIVRGEELDAHSNPMLQEAQQWGMELAFVSRQRYREWTRADGPA
                     ADAHTWIIPEGGDNHLGVLGCMSLVPRAAYAGYDQVWLALGTGCTLAGVRLALPPTVN
                     VCGVSVLAGDWPRQQMQERLQQWNWHEQANWDVLTLYHAGGYGRAPAAVLSFVAEFGD
                     NTGVPLDPVYTGKAMQALCDQVQRGIVSAGTRVLFIHTGGLQGARGFSSSTG"
     gene            649..1533
                     /locus_tag="VFX11_17125"
     CDS             649..1533
                     /locus_tag="VFX11_17125"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEX4940398.1"
                     /translation="MADKDSFRNIGLIARPGSPQVVDTLQTIIAFLDGRNIKVMLDED
                     MKDMMPLDGHQRGNRKMIGTTCDLVIVVGGDGCLLGAARDLARYKVPLLGVNRGRLGF
                     LTDILPSEVEERISEVLDGEYTTEYRFLLEAQIKRDGKLMGDNSALNDVVFHSGNTVK
                     MLEFELFVDGDFVYRQRSDGLIVSTPTGSTAYALSGGGPIMHPRLDAIVMVPMFPHTL
                     SSRPIVIDGNSEIKIYVSPTNEVHPLLNCDGQRGAVLDPGDWVYIRKRSHPLKLLHPS
                     SHSFYEACRSKLGWSEVL"
     gene            1530..2495
                     /locus_tag="VFX11_17130"
     CDS             1530..2495
                     /locus_tag="VFX11_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX4940399.1"
                     /translation="MIIDRDDVAGYDVIGDVHGCARALCQLLQELGYQEQHEVWRHPE
                     RKAVFVGDIIDRGTQIRDAMRLVRRMVEAGSAYLALGNHEYNAVAFAFEVDGPADCPR
                     RQFQLRLARHLQQTLQEYRFHEAEWRDTLDWLRQQPLCLEFSHFRVVHACWDALRIGQ
                     ARAIHNGCALRDEQFLLDSMQRGTEPYRVVERLLKGTDLRLPEGMTLIGADGIERNRI
                     RTKFWKKTPATYGDVLFQPDKLPDEWMQRALSDQDKERLLHYGKQEKPLFVGHYWRRG
                     TPKVIRDNIACLDYSAVKNGRLVAYRMGAEARLYNEQFAWVSVSL"
     gene            2592..4292
                     /locus_tag="VFX11_17135"
     CDS             2592..4292
                     /locus_tag="VFX11_17135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940400.1"
                     /translation="MNLKGTAASLLLWLLLTLALVLMSGWMHQLANRTPSHQDARQIN
                     LTLQYEVQQLQDLLHWVATLPAEELAGIDTPADLRLIVDSSDVLLFHVSSDWEKERQA
                     LLVSLVNFLNQPLEQNLGVTYWRDQVLLVAVHRDGDRKYLAGMFLNGWLRRLVDATGI
                     QLRLVTGEIVDVLRRSGHAVLPLPAMLGKPVYIEGKPVLLNEAIPFRWWLAVPTATVV
                     AALIVWFFHYRPVWRRLRGLLLQMRGIMQGSTFRERLQPSGKDEIGELVVQFNSLLSS
                     LEYSYNLMAKTNLVTTELLSRVDVAPAALPQPTDEQALKQSLDMASRLSGALQRKAIE
                     LYLQPVFGRDRTTVTGYEALSRWMDPELGMVLPLEYLAVAEKAGLTDPLTELMLHQTF
                     DLLRRLPGSDQEPLMLSVNLSAAQLFAPGLMQFLERCEKEDRKLFARLELEVKESTLT
                     RDFDLAAVIVGRLRELGIGVCIDDFGLSRYSLMYLQKLPVTAIKLARVFSERISREPR
                     EVAFIEGVARFAGGLGVRVIVKNLENEQQLLSLRPDLPVEYQGLALGGPMPVADVIRR
                     "
     gene            4356..4649
                     /locus_tag="VFX11_17140"
     CDS             4356..4649
                     /locus_tag="VFX11_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HEX4940401.1"
                     /translation="MTLDELIASITPETYANLQRAVELGKWPDGTRLTPEQRANCMQA
                     VIAYGERNLAAEERVGYIDRGVKEEGEVCGDDHSQADTHDHAHEQEKPVKFLN"
     gene            complement(4695..5147)
                     /locus_tag="VFX11_17145"
     CDS             complement(4695..5147)
                     /locus_tag="VFX11_17145"
                     /inference="COORDINATES: protein motif:HMM:NF040030.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HEX4940402.1"
                     /translation="MPKYPLPVQAIIDQEQLQQVLWVTAAGGDDSDAPVQPMDTVLQD
                     DFQQRFDLVFIDARCFAQPKTRLLALIAKARDLLASRVLVEWQPEVHREWQDADFLAL
                     AMRRRAVMETGTGPQVYYGYDLRTYKQVPDWLNPKYWANPQMWDQARW"
BASE COUNT         1006 a         1489 c         1712 g         1049 t
ORIGIN      
        1 tcggcattgt gcggggtgaa gaactggatg cccattccaa cccgatgctg caggaagcgc
       61 agcaatgggg catggagctg gcgtttgtgt cgcgtcaacg ttatcgcgaa tggacccggg
      121 ctgacgggcc ggcggcggat gctcacacct ggattattcc tgaaggcggc gacaatcacc
      181 tgggtgtgct gggttgcatg agtttggtgc cgcgcgcagc atacgccggg tacgatcagg
      241 tctggctggc gctgggtacc ggttgcacgc tggcaggtgt gcggctggcg ttaccgccga
      301 cggtgaacgt gtgcggcgta tcggtactgg cgggcgactg gccccggcag cagatgcagg
      361 aacgtttgca gcagtggaac tggcacgagc aggcgaactg ggacgtgctg acgctgtatc
      421 atgcaggtgg ttacggccgt gcgccggcag cagtgttgag ttttgtcgct gaatttggcg
      481 acaataccgg cgtgccgctg gacccggtct ataccggtaa agcgatgcag gcattatgcg
      541 atcaggtgca gcgtggcatt gtttctgctg gcactcgcgt gctgttcatt cataccggcg
      601 gtctgcaagg cgcgcgcggt ttttcatcct ccacaggata agttggcaat ggcagacaaa
      661 gattcctttc gcaatattgg tttgatcgcc cggcccggca gtccgcaggt ggtggacacg
      721 ctgcagacca tcatcgcgtt tctggatggt cgcaacatca aggtaatgct tgacgaagac
      781 atgaaggaca tgatgccgct cgacggtcat cagcgcggca atcgcaagat gatcggcacc
      841 acctgcgatc tggtgatcgt ggtgggcggc gatggttgcc tgctgggtgc ggcgcgggat
      901 ctggcccgtt acaaggtgcc gttgctgggg gtgaaccggg gccggctggg attcctcacc
      961 gatattctgc cgagcgaggt ggaagaacgc atcagcgaag tgctggatgg cgagtacacc
     1021 accgaatacc gctttctgct ggaagcgcag atcaagcgcg acggtaaatt gatgggggat
     1081 aattccgcgc tcaacgacgt ggtgttccat tccggcaaca cggtgaagat gctggagttc
     1141 gaattgttcg tggatggcga tttcgtttat cgccagcgct cggatggtct cattgtgtcg
     1201 acgcccaccg gttccaccgc ctatgccttg tccggcggcg ggccgatcat gcatccgcgt
     1261 ctggatgcga tcgtgatggt gccgatgttt ccgcacacgc tgagcagtcg gccgatcgtg
     1321 atcgacggca acagcgagat caagatctat gtgtcgccca ccaatgaggt gcatccgttg
     1381 ctgaactgcg acggccagcg cggcgcggtg ctcgatccgg gcgactgggt gtacatccgc
     1441 aagcgttccc atccgctcaa gctgctgcat cccagcagcc acagttttta cgaagcctgc
     1501 cgctccaagc tgggctggag cgaagttctt tgatcatcga ccgcgatgat gtggccggtt
     1561 acgacgttat tggcgatgtg cacggctgtg cccgggcact gtgccagttg ctgcaggaac
     1621 tgggctatca ggaacagcat gaggtgtggc ggcatccgga acgcaaagcg gtgttcgtcg
     1681 gcgatatcat tgatcgcggc acgcagatcc gtgatgccat gcgtctggtg cggcggatgg
     1741 tggaggcggg cagtgcctat ctggcgctgg gcaatcatga atacaacgcg gtggcgtttg
     1801 cctttgaagt agacgggccg gcggactgtc cgcgccggca gttccagttg cggctggccc
     1861 ggcacctgca gcagaccctg caggaatacc gttttcacga agcggaatgg cgtgacaccc
     1921 tggactggct gcgccaacag ccgctctgtc tggaattttc ccattttcgc gtggtgcatg
     1981 cgtgctggga cgcgctgcgg atcgggcagg cccgcgcgat ccacaatggc tgcgcactgc
     2041 gcgacgaaca gttcctgctc gacagcatgc agcgtggcac cgagccgtac cgggtggtgg
     2101 aacgcctgct caagggtacc gacctgcgct tgccggaggg catgacgctg attggtgctg
     2161 acggcatcga gcgcaaccgc atccgcacca aattctggaa aaaaacgccc gccacctatg
     2221 gcgacgtgct gttccagccg gacaagctgc cggatgaatg gatgcagcgg gcgttaagcg
     2281 atcaggacaa ggaacgcctg ctgcattatg gcaagcagga aaagccattg ttcgtgggac
     2341 actactggcg tcggggtacg ccgaaagtga ttcgtgacaa tatcgcctgt ctggattaca
     2401 gcgcggtgaa aaacggccgg ctggtggcct accggatggg cgcggaagcg cggctttaca
     2461 acgaacagtt cgcctgggtg agcgtcagtc tgtgacagca attgcggaaa atgctcctac
     2521 acttaaccgc acgaaaacag aacaatacaa agcaataaaa acaggaatca gatgaaagga
     2581 atttcaccga catgaatctg aaggggactg ccgcttctct gttgttgtgg ctgctgctga
     2641 cattggcgct ggtgctgatg agcggctgga tgcaccagct ggccaatcga acgccgtcgc
     2701 atcaggacgc gcgccagatc aatctcaccc tgcaatacga agtgcaacag ctgcaggatc
     2761 tgctgcactg ggtggcgacc ttgccggcgg aggaactggc cgggatcgat accccggccg
     2821 atctgcggct gatcgtcgac agcagtgatg tgctgctgtt tcatgtcagt tccgactggg
     2881 aaaaagagcg ccaggcgctg ctggtgagtc tggtgaattt tctcaatcag ccgctggagc
     2941 agaatctggg cgtgacctat tggcgcgacc aggtgctgct ggtggcggtg caccgtgacg
     3001 gcgaccggaa atacctggcc ggcatgttcc tgaatggctg gctgcgccgg ctggtggacg
     3061 ccaccggcat ccagttgcga ctggtgacag gcgagatcgt cgatgtgctg cgtcgcagcg
     3121 gacatgcggt gctgccactg ccggccatgc tgggcaagcc ggtgtatatc gaaggcaaac
     3181 cggtgctgct gaacgaggcg attccgttcc gctggtggct ggcggtgccg acggcaacgg
     3241 tggtggcggc cctgatcgtg tggtttttcc attaccggcc ggtttggcgc cgtctgcgcg
     3301 gcctgctgct gcagatgcgc ggcatcatgc agggcagtac cttccgcgag cgtctgcaac
     3361 cttctggcaa ggacgagatc ggcgaactgg tcgtgcagtt caacagcctg ctcagttcgc
     3421 tggaatacag ttacaacctg atggcgaaaa ccaatctggt gacgacggaa ttgctgtcgc
     3481 gggtggatgt ggcgccggcc gcgttgccgc aacccacgga tgagcaggcg ctgaaacagt
     3541 cgctcgatat ggcgtcccgc ctgagcgggg cgctgcagcg caaagccatc gaactctatc
     3601 tgcaaccggt gttcgggcgc gaccgcacca ccgtcaccgg ctatgaggcg ctgagccgct
     3661 ggatggaccc ggagctgggc atggtattgc cgctggaata cctggcggtg gcagaaaagg
     3721 ccggactcac cgaccccctc accgaactga tgctgcacca gacgttcgac ctgttgcgcc
     3781 gcctgcccgg cagcgatcag gaacccctga tgctgtccgt caacctgagc gcggcgcaac
     3841 tgttcgctcc tgggctgatg cagttcctgg aacggtgcga aaaggaggac cgcaagctgt
     3901 tcgcgcgcct ggaactggaa gtgaaggaat ccaccctgac ccgggacttc gatctggcgg
     3961 cagtgatcgt ggggcggctg cgggaactgg gcattggtgt ctgcatcgat gatttcggcc
     4021 tcagccgtta ttcactgatg tatctgcaga agctgccggt cacggccatc aagctggcgc
     4081 gggtgttcag cgaacgcatt tcccgcgaac cccgcgaggt ggctttcatc gagggcgtag
     4141 cccgctttgc tggcggcctg ggggtgcggg tgatcgtcaa gaatctggag aacgagcagc
     4201 aactgctgag cctgcggccg gaccttcccg tggaatatca gggacttgcc ctgggtggtc
     4261 cgatgccggt tgccgacgtg atccggcgtt gagcaccgtc tcaacccggc gcaacatcga
     4321 tggtatactg cgcgcctctg cctgcggagc cgatcatgac cctcgacgaa ctcattgcct
     4381 ccattacccc ggaaacctac gccaacctgc agcgtgccgt tgagctgggc aagtggcccg
     4441 acggcacccg cctgacgccg gagcagcgtg ccaactgcat gcaggcggtg atcgcctacg
     4501 gcgagcgcaa tctggcggca gaggagcggg ttggttacat cgaccgggga gtgaaggaag
     4561 aaggggaagt gtgtggcgat gaccattccc aagcggatac ccacgatcat gcccacgagc
     4621 aggaaaagcc ggtcaaattt ctgaattgat tctcaccgcg gtgcgcgcat gccgttgcgc
     4681 gcaggctgcg tgatctacca gcgcgcctga tcccacatct gcgggttcgc ccagtatttg
     4741 ggattgagcc agtccggcac ctgcttgtag gtgcgcagat cgtagccgta atacacctgg
     4801 ggaccggttc cggtctccat caccgcccgc cgccgcatgg ccagggccag aaaatccgca
     4861 tcttgccatt ccctgtgcac ttccggctgc cactccacca gcacccggct ggccagcaga
     4921 tcccgcgcct tggcgatcag cgccagcaac cgcgttttcg gctgcgcaaa acagcgggcg
     4981 tcaatgaaca ccagatcgaa gcgctgctga aaatcatcct gcagaacggt gtccattggc
     5041 tggactgggg cgtcgctgtc atcgccgccg gcggccgtca cccacagcac ctgctgcaac
     5101 tgttcctgat cgataatggc ttgaactggc aggggatatt tcggcataag gtcatatttg
     5161 cgtaagcggc tggggctggg acatactgcg tgacattctc ctttttcaca agtcgccctt
     5221 tttacaggca tccgcattat ctccgcagga attcgt
//
LOCUS       DASUEG010000906         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1338036, whole genome shotgun sequence.
ACCESSION   DASUEG010000906 DASUEG010000000
VERSION     DASUEG010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338036"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1013
                     /locus_tag="VFX11_17150"
     CDS             93..1013
                     /locus_tag="VFX11_17150"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX4940403.1"
                     /translation="MSMADRDGLIWFDGEMVPWRDAKVHVLTHTLHYGMGVFEGVRAY
                     ATANGPGIFRLQEHTDRLFRSAHIMNMKIPFTKEQVNEAQLAAVRENKLPHAYLRPMV
                     FFGSEGMGLRASNLQVHLIVAAWEWPSYMSPEAKEVGIKVRTSSYTRHHVNISMCKAK
                     ANGHYINSMLALNEALACGCEEALLLDTEGYVAEGSGENVFIVYKGKIYTPELTACLD
                     GITRATVMQLANELGYEVIEKRITRDEVYVADEAFFTGTAAEVLPIQQLDGRVIGSGK
                     RGPITEQLQKLYFDAVMGRSEKHKDWISYI"
     gene            1089..2009
                     /locus_tag="VFX11_17155"
     CDS             1089..2009
                     /locus_tag="VFX11_17155"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX4940404.1"
                     /translation="MTAQAAKPTILWVVTDEKPGHRSQQEGLVERLQALAPFEIHWLP
                     VDTIQISLLDAVLRRRVMPNLPAPDWILGAGAGTHSLILMLKRIFRAKTILLMKGAFP
                     AALFDANITPLHDNPPTRKNVLPTTGVMNPVFPRYEGRDKNTGTFLIGGINEHYGWDD
                     GTVIDQVEKICRAQSGVQWTLTDSRRSPAAFLPALKARNLPNLTLISHKDTARGFIKQ
                     QLDTTGQLWVTRDSVSMVYECITSGAPTGLLDLKPLRQSRVVKNMDELLKEGWIQDFA
                     LWDLRQPLPPSPRRLWEADRAARWLLQFFG"
     gene            complement(2016..>2939)
                     /locus_tag="VFX11_17160"
     CDS             complement(2016..>2939)
                     /locus_tag="VFX11_17160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4940405.1"
                     /translation="RQWEHRMDTLRRNGVETEGFRFGGKLDMLDTWAYRRALLEFHPD
                     IVMTWMGRASIATPAGKGRYILVNRLGHYYNLKYYRHADYWVGISRGICKHMVDGGMP
                     GDRVFYIPNFADETPVQPIPRDSFNTPTDRPLILAAGRLHENKAFEVLLQAMTDLPGV
                     TLWLAGSGPEEANLKQLCNRLGLDEQVRFLGWRNDVTTLMASVDLFVCPSRHEGLGSI
                     VMESWAHRCPIVATNSQGPGEAITDGVSGLITPVDDAKALGQAIRRVIESPDLRQALI
                     EGGSRVYAEGYCRQHIVEEYVKLYQQIMARG"
BASE COUNT          609 a          924 c          848 g          558 t
ORIGIN      
        1 agtggccggc ctcagccgcc gccaacgcgg cctaaatgct ttcaacctac tgcattcaaa
       61 gacaagcctg taatcgatag gagagtttca ccatgtccat ggccgaccgc gacggtctga
      121 tctggtttga cggcgaaatg gtgccctggc gcgatgccaa ggtccacgtg ctgacccaca
      181 ccctgcatta cggcatgggc gtgtttgaag gcgtccgcgc ctacgccact gcgaacggcc
      241 cgggcatctt ccgcctgcag gaacataccg accgcctgtt ccgctccgcg cacatcatga
      301 acatgaaaat tcccttcacc aaggaacagg tgaacgaggc gcagctggcc gccgtacggg
      361 aaaacaagct gcctcacgcc tacctgcggc cgatggtgtt cttcggttcc gaaggcatgg
      421 gcctgcgtgc ttccaacctg caggtgcacc tgatcgttgc cgcgtgggaa tggccgtcct
      481 acatgtcgcc ggaagccaag gaagtgggca tcaaggtgcg cacctcgtcc tacacccgcc
      541 accatgtgaa catctccatg tgcaaggcaa aggcgaacgg tcactacatc aactccatgc
      601 tggcgctgaa cgaagccctg gcctgcggtt gtgaggaagc cctgctgctg gacaccgaag
      661 gctacgtggc tgaaggcagc ggcgagaacg tattcatcgt ttacaagggc aagatctaca
      721 cgccggaact gaccgcgtgc ctggacggca tcacccgcgc cacggtcatg cagctggcca
      781 acgaactggg ctacgaagtc atcgagaaac gcatcacccg cgacgaagtc tacgtggcag
      841 acgaagcctt cttcactggc actgccgccg aagtgctgcc gatccagcaa ctggatggcc
      901 gcgtcatcgg ttccggcaag cgtggcccga tcaccgagca attgcagaaa ctgtatttcg
      961 atgcagtgat gggtcgctcc gagaagcaca aggactggat cagctatatt tgatccgtcc
     1021 gcctgtggcc gcgaggaatc tcaccttgcg gccacgtcct tccgcatacc ctgcctgaag
     1081 gggccgccat gacagcccaa gccgccaaac ccaccattct ctgggtcgtc accgatgaaa
     1141 aacccggcca tcgcagccag caggaaggcc tggtggagcg cctgcaggcc ctggcaccgt
     1201 tcgaaataca ctggcttcct gtcgatacca ttcagatcag tctgctcgat gctgtgctgc
     1261 gccgtcgggt gatgccgaac ctgccggcac cggactggat tctgggggcc ggcgccggca
     1321 cccacagcct gatcctgatg ctgaagcgaa tcttccgcgc caaaaccatt ctgctgatga
     1381 agggtgcgtt tccggctgcg ctgtttgacg ccaacatcac gccgctgcat gacaatccgc
     1441 caacgcgcaa aaacgtgctg ccgaccacgg gcgtgatgaa cccggtattt ccccgttatg
     1501 aaggccgcga taaaaacacc ggcacatttc tgattggtgg cattaatgag cattacggct
     1561 gggacgatgg gacggtcatc gatcaggtgg aaaaaatctg tcgtgcccag tccggcgtgc
     1621 agtggacgct gaccgattcc agacgctcgc cggcagcatt cctgccggca ctgaaagcca
     1681 gaaacctgcc caatctgacg ctgatttccc acaaggacac cgcacgcggc ttcatcaagc
     1741 agcaactgga taccaccggc cagctgtggg tgacccgtga cagcgtgtcg atggtgtatg
     1801 aatgcatcac ttcaggtgcg cccacgggcc tgctggacct gaagccgttg cgccagtcgc
     1861 gggtggtaaa aaacatggat gaattgctga aggagggctg gatccaggat ttcgcgctgt
     1921 gggatctgcg tcagccgttg ccgccatcgc ccaggcgatt gtgggaagcg gaccgggcgg
     1981 cgaggtggtt gttgcagttt tttgggtgac gcgcgtcaac cgcgcgccat gatctgctgg
     2041 tacagcttca catactcctc cacgatgtgc tggcggcaat aaccctcggc atacacccgg
     2101 ctgccaccct cgatcagcgc ctgccgcaga tccggacttt caatgacccg gcggattgcc
     2161 tggccgaggg ctttggcatc gtccaccggt gtgatcagac cgctcacacc atccgtaatt
     2221 gcctcgcccg gcccctgcga attggtggcg acgatagggc agcgatgagc ccaggattcc
     2281 atcacgatcg accccagccc ctcgtgccgg gacggacaca cgaacagatc caccgatgcc
     2341 atcagcgtgg tcacgtcgtt gcgccagccc agaaagcgca cctgctcatc caggcccaac
     2401 cggttgcaca gctgtttcag attggcttct tccgggcctg atcctgccag ccacaacgtg
     2461 acgcccggca aatccgtcat cgcctgcagc agcacttcaa aggccttgtt ctcgtgcagg
     2521 cgccctgctg ccagaatcag cgggcggtcg gtaggcgtgt tgaaactgtc gcgcgggatc
     2581 ggctgcaccg gcgtttcatc ggcgaaattg ggaatgtaga acacgcggtc gccgggcatg
     2641 ccgccatcga ccatgtgttt gcagatgccc cgactgatgc caacccagta atcggcatgc
     2701 cggtaatatt tcaggttgta gtaatgcccc agccggttca ccagaatata gcgacccttg
     2761 ccggcgggcg tggcaatgct ggcgcgaccc atccaggtca ttacgatgtc ggggtgaaat
     2821 tccagcagtg cacgccggta ggcccaggta tccagcatat ccagtttgcc gccgaaccgg
     2881 aaaccttcgg tttcaacacc atttctgcgc aaggtgtcca tgcgatgttc ccactggcg
//
LOCUS       DASUEG010000907         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2067511, whole genome shotgun sequence.
ACCESSION   DASUEG010000907 DASUEG010000000
VERSION     DASUEG010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067511"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFX11_17165"
     CDS             <1..327
                     /locus_tag="VFX11_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940406.1"
                     /translation="VIPYVNDRIAFGEPISNRQSVAFMVANMAIELDAMRLMVYRAAS
                     RAEQGLEFHQQAYLCRVLCAERSMEIGTNGVQLLGGHGFTREHPVEMWYRNLRAVGIL
                     LGVAAV"
     gene            373..1608
                     /locus_tag="VFX11_17170"
     CDS             373..1608
                     /locus_tag="VFX11_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940407.1"
                     /translation="MINLELPQKLLTAQQMAHQLAKGAFRPIARKYDKIEHCPTPEEL
                     IPISQMMRAMGGQGALGGKKEGGKKEGSKSEAAADSVKNGANLMGIMATTEMAWGCCG
                     LMLSLPGVGLGNAAIDAVGTPEQKEKFGKLYCAMAITEPGCGSDSAAVQTTAKLDGDE
                     WVINGEKIFATAGDRGDAVVVWATLDKSLGKAAIKSFVVEKGTPGMEVVRCEDKMGLR
                     VSDTAAIRFHDCRIPKSNILGSPEIAADAEARKKAFGGVMQTFDNTRPMVAAMALGVA
                     RGALEITRELLEKEGVKADYSKSLYNSSALEAEIYRMEANLEGARLLTLKAAWMADNK
                     QPNSKEASMCKAKAGRVGNDITLKCVEICGVLGYSENWLLEKLARDSKIIDIFEGTQQ
                     IQQLIVARHLLGKSSSELK"
     gene            1776..4451
                     /locus_tag="VFX11_17175"
     CDS             1776..4451
                     /locus_tag="VFX11_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940408.1"
                     /translation="MPFSESPGQLRETLGNLFARTAACLRYPARMSFYVAQAGHIDEI
                     QILPSTELMLEIHDDITPLSSYPHAVLESVFRTGNPILVEQSQLSEISNLDVYVVNAG
                     ITSFLCLPVVATEACNAAIWLERKDPLQNFSFAELDSLEQQIHGLQDRLTHHIETQSL
                     HRLFNRDLFESELVFVSRDNAFMEGVRAVESHLRVFERAVKSADTGFVIVDARLPNQP
                     IIYCNPAFERITGYSFRETVGKNCRFLQKPGEQDEKVQLMRDAILRGEPCQVLLKNYR
                     KNGELFWNELTISPIRDAQGNLTHLIGVQNDVSDRVAIDVARKETESRFRALYDNSLD
                     GICYYSLEGRCISANRAYCDLLGLTPEEVVGIHYADITPEKWREADRKVVQEQLFVRG
                     YSDLYEKEYFRKDGSIVPVSVRAALHYDASGKPIATWGIVRDISEYRNVIDRLKQNEA
                     HMEFLAYHDSLTAMPNRLLFQDRVIKALERAKRNGKKVALMLLDLDRFKNINDSLGHA
                     MGDSFLKEISTRIQHTIREMDTAARIGGDEFVIVMENFNEISSVTAVANKLLKTLAVP
                     INIQQHELYATASIGISIFPNDGQNVETLMKAADAAMYCAKEKGKNSFHYYTDDLNKR
                     TLELLVLENDMRRALDLNQFFVAYQPQFALQDGALVGVEALLRWQHPVQGLLAPTVFI
                     PLAEETGLIEPLGRWMLRASCTQIKAWVEKGYRMGRIAVNLSARQFRQPGLVEDIHFI
                     LSDVGLGAEHLELEITESIAMDNVESTVAKLEVLKRMGLRLSIDDFGTGYSSLSYLKR
                     FAIDKLKIDQSFVRDIVTDPNDAAIAASTIALAHKMGLKVIAEGVETAEQVAFLREQG
                     CDEVQGFFYGKPMSAVELERMLGTAPQRYRAGAQH"
     gene            complement(4467..>5228)
                     /locus_tag="VFX11_17180"
     CDS             complement(4467..>5228)
                     /locus_tag="VFX11_17180"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX4940409.1"
                     /translation="QFGNLVDLFMLDGRTYRTPHPCGEGDFFGRYIPIGCQNWASDQQ
                     TLLGGDQRDWLLNGLIGSSARWKLLGNQTYMGRLGIIMKNGRIPINVDAWDGFDYERN
                     LINNEVASNKVENFVVVTGDLHTYIASHVKKDYSRNSIFDFHNYLGVEFMTPSVTSAG
                     LFDMIGAKTPVGQQQQLAQALTNTAVRLTNPHIRYFNTTQNGWSTIEFNNRQCDWKAY
                     VVDKNINSGTVNNKLIRHYRKYTTNPWITTQAAFI"
BASE COUNT         1156 a         1407 c         1564 g         1101 t
ORIGIN      
        1 gtgattccct atgtgaacga ccgcatcgcg tttggcgaac cgatttccaa ccgtcagtcc
       61 gttgccttca tggtggcgaa catggcgatc gaactggatg ccatgcgcct gatggtgtac
      121 cgggccgcgt cccgtgccga gcagggcctg gagttccacc agcaggcgta tctgtgccgc
      181 gtgctgtgcg ccgagcgcag catggaaatc ggtaccaacg gagtgcagtt gctgggtggc
      241 cacggtttca cccgcgagca tccggtggaa atgtggtacc gcaacctgcg tgcggtgggc
      301 atcctgctgg gcgtcgccgc tgtttaagcg ggcagtgatt tatccggtcg aatcgaataa
      361 cggagaacga acatgatcaa tctcgaatta ccgcaaaaac tgctgactgc acaacagatg
      421 gcgcaccagc tggcgaaagg cgcgttccgt cccatcgccc gcaaatatga caagatcgaa
      481 cactgcccca caccggaaga actgattccc atcagccaga tgatgcgggc catgggtgga
      541 cagggggctc tcggcggaaa aaaagagggg ggaaagaaag agggcagcaa gagtgaggcc
      601 gctgccgaca gcgtgaaaaa cggtgccaac ctgatgggca tcatggccac caccgaaatg
      661 gcgtggggtt gctgcggcct gatgctgtcg ctgccgggcg tgggcctggg caacgccgcg
      721 atcgatgcgg tgggcacgcc ggagcagaag gaaaaattcg gaaaattgta ctgcgcgatg
      781 gcgatcactg aacccggttg cggttccgat tccgccgccg tgcaaaccac ggcaaaactg
      841 gatggtgatg agtgggtcat caatggcgaa aaaatctttg ccactgccgg cgatcgtggt
      901 gacgcagttg tagtgtgggc caccctcgac aaatccctgg gcaaggctgc catcaaatcc
      961 ttcgtcgtag aaaaaggcac tcccggcatg gaagtggtgc gctgcgaaga caagatgggt
     1021 ctgcgcgtgt ccgacaccgc cgccatccgt ttccacgact gccgtattcc caaatccaac
     1081 atcctgggct cccctgaaat tgctgccgat gccgaagcgc gcaaaaaagc cttcggtggc
     1141 gtgatgcaga ccttcgacaa cacccgtccg atggtggcgg cgatggcgtt gggtgtggcc
     1201 cggggcgcgc tggagatcac gcgtgagctg ctggaaaaag agggcgttaa agcggattac
     1261 agcaagtcgc tttacaacag cagcgcgctg gaggcggaaa tctaccgcat ggaggccaat
     1321 ctggaaggtg cccgcctgct gaccctgaaa gcggcctgga tggcggacaa caagcagccg
     1381 aactcgaagg aagcgtcgat gtgcaaggcg aaagccggcc gcgtcggtaa cgatatcacc
     1441 ttgaaatgcg tcgagatttg cggcgttctg ggatacagcg aaaactggct gctggaaaag
     1501 ctggcgcggg attcgaaaat catcgacatc ttcgaaggca cgcagcagat ccagcaattg
     1561 attgtcgccc gtcacctgct gggcaagtcc tccagcgaac tgaaataagc gcgcagctgt
     1621 tgctgcggcc cattggccaa agcaactggc ttacttttcc attctgttgt caacaacgca
     1681 gccggacact caggtgtccg gttagcgccc gtttttccag cgattgccta tactgaacgc
     1741 attatctgca tcaattgggg tggcttcatc gcgttatgcc gttttcggaa tcgccaggcc
     1801 agctgcggga aaccctgggc aatctgtttg ccaggactgc cgcgtgcctg cggtatccgg
     1861 cgcggatgtc cttttatgtg gcacaggctg gccatattga tgagatccag atattgccca
     1921 gcacggagct gatgctggag atccatgatg acatcacccc cttgtccagt tatccgcacg
     1981 cggtattgga aagtgtgttt cgcaccggca atcccattct ggtggaacaa agccagctga
     2041 gcgagatcag taacctggac gtatatgtcg tcaatgccgg aatcaccagt tttctttgcc
     2101 tgccggtggt ggccaccgag gcctgcaacg ccgccatctg gctggagcgc aaggatccgc
     2161 tgcagaattt cagttttgcc gaactggata gcctggaaca gcagattcac gggctccagg
     2221 atcggctgac ccatcatatt gaaacccagt ctctgcaccg tcttttcaat cgcgacctgt
     2281 ttgagtccga gctcgtgttc gtttcccggg acaatgcatt catggaaggg gtgcgggcgg
     2341 tggaatcgca tctgcgggta tttgagcggg cagtaaaatc tgccgacacg ggctttgtga
     2401 ttgtcgatgc acgattgccc aatcagccga tcatatattg caatcccgcc ttcgaacgca
     2461 ttaccggtta ttcctttcgg gaaaccgtgg gcaagaactg ccgtttcctg cagaagccgg
     2521 gcgagcagga cgaaaaggtc cagctgatgc gggatgcgat actgcgcgga gaaccttgcc
     2581 aggtattgct gaaaaattat cgcaaaaatg gcgaactgtt ctggaatgaa ctcaccattt
     2641 cgccgatcag ggatgcgcag ggcaatctta cccatctgat tggcgtgcag aacgatgtca
     2701 gtgaccgcgt ggcaatagat gtggcgcgca aggaaactga atcccgtttt cgtgccctgt
     2761 acgacaatag cctggatggc atctgctact acagcctgga aggccgctgt atcagtgcca
     2821 atcgtgctta ctgcgatctg ctgggtttga cgcccgagga agtcgtcggc attcactacg
     2881 ccgatattac ccccgaaaag tggcgggaag cggatcgcaa agtggtccag gaacaattgt
     2941 tcgtccgggg ctatagcgat ttgtatgaga aggaatactt tcgcaaggat ggcagcatcg
     3001 ttccggtcag tgtgcgtgcg gcgctgcact atgatgccag cggaaaaccc attgccacct
     3061 ggggaatcgt gcgcgacatc agcgaatacc gcaacgtcat cgaccggctg aagcagaatg
     3121 aagcgcacat ggaatttctg gcctatcacg attcactcac ggccatgccc aaccgcctgc
     3181 tgtttcagga tcgcgtgatc aaggcgctgg aacgggccaa gcgcaacggc aaaaaagtgg
     3241 cactgatgct gttggatctc gatcgtttca agaacatcaa tgattctctc ggccacgcga
     3301 tgggcgacag tttcctcaag gaaatttcca cccgcatcca gcataccatc cgcgaaatgg
     3361 acactgcagc gcgcatcggt ggcgacgaat tcgtgatcgt gatggaaaat ttcaacgaga
     3421 tcagcagcgt gacggcggtg gccaacaaac tgctgaaaac gttggctgtg cccatcaata
     3481 tccagcagca tgaactgtat gccactgcca gcatcggcat cagcattttt cccaacgacg
     3541 gccagaatgt ggaaaccctg atgaaggctg ccgatgccgc catgtactgc gccaaggaaa
     3601 aagggaaaaa cagttttcac tattacaccg acgatctcaa caagcgcacc ctggaattgc
     3661 tggtgctgga aaatgacatg cggcgtgcgc tggacctgaa ccagtttttt gttgcctacc
     3721 agccgcagtt tgccctgcag gatggcgcgc tggtgggtgt ggaagccttg ttacgctggc
     3781 agcatccggt gcagggcttg ctggcgccaa ccgtgttcat tccactggcc gaggaaaccg
     3841 gattgattga accgctcggt cgctggatgc tgcgtgccag ttgcacccag atcaaggcgt
     3901 gggtggagaa gggctaccgc atggggcgga tcgcggtgaa cctgtccgcg cgccagttcc
     3961 gtcagccggg gctggtggag gacattcatt tcatcctcag cgatgtaggc ttgggcgccg
     4021 agcacctgga gctggaaatc accgaaagca tcgcaatgga caatgtcgaa tccacggttg
     4081 caaaactcga agtgctgaaa cggatgggat tgcgcctgtc gatcgacgat tttggtaccg
     4141 gatattcctc gctgagttat ctcaagcgtt tcgccatcga caaattgaag attgaccaga
     4201 gtttcgtgcg cgacatcgtg actgatccca acgacgccgc catcgcggca tccactatcg
     4261 cactggccca caagatgggt ttgaaagtga ttgcggaagg tgtggagacg gcggagcagg
     4321 tggcgttcct gcgggagcag ggttgcgatg aagtgcaggg gtttttctac ggcaagccaa
     4381 tgtcggccgt ggagctggag cgcatgctgg gcacggcgcc gcaacgttac cgcgccggcg
     4441 cccagcattg agttaccgcg ttcggatcag atgaacgctg cctgcgtggt gatccaggga
     4501 ttggtggtgt acttgcggta gtggcggatc agcttgttgt tcaccgtgcc actgttgata
     4561 ttcttgtcca ccacgtaggc tttccagtca cactgccgat tattgaattc aatcgtcgac
     4621 cagccattct gcgtggtatt gaaataacgg atatgggggt tggtcagccg cacggcagtg
     4681 ttggtcagcg cctgcgccag ttgctgctgt tgtcctaccg gtgttttcgc cccgatcata
     4741 tcgaacagcc ccgccgaggt caccgacggc gtcatgaatt caacgcccag ataattgtga
     4801 aaatcgaaaa tggaattgcg gctgtaatcc tttttcacgt gtgacgcgat ataggtgtgc
     4861 aggtcgcccg tcaccaccac gaagttctcc accttgttgg atgccacttc attgttgatc
     4921 aggttgcgct cgtaatcgaa gccgtcccac gcgtccacgt tgatggggat gcggccgttt
     4981 ttcattatga tgccaagccg gcccatgtag gtctggttgc ccagcagttt ccagcgtgcg
     5041 ctggagccga tcaggccgtt gagcagccag tcgcgctgat cgccgcccag caacgtttgc
     5101 tggtcactgg cccagttctg gcagccaatc ggaatgtagc ggccgaagaa atcgccttca
     5161 ccgcagggat gcggcgtgcg gtaggtgcgg ccgtcgagca tgaacaggtc caccagattg
     5221 ccgaattg
//
LOCUS       DASUEG010000908         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1205938, whole genome shotgun sequence.
ACCESSION   DASUEG010000908 DASUEG010000000
VERSION     DASUEG010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205938"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..378
                     /gene="apaG"
                     /locus_tag="VFX11_17185"
     CDS             19..378
                     /gene="apaG"
                     /locus_tag="VFX11_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX4940410.1"
                     /translation="MAVEVISEYIPDQSNAAEHRFVFAYHVRIENRGNLPAQLLSRHW
                     IITDGNQRVQEVRGEGVIGEQPTILPGETYQYSSGAVLQTPVGSMKGSYQMVDATGIR
                     FDAPIPIFTLASPTALH"
     gene            378..1196
                     /locus_tag="VFX11_17190"
     CDS             378..1196
                     /locus_tag="VFX11_17190"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX4940411.1"
                     /translation="MATWAIGDLQGCYEDFRCLLEKIRFNADRDQLWLTGDLVNRGPA
                     SLDTLRFCHERRDNLTTVLGNHDLHMLAVLSGSIEFKRKDTFRDVVKASDRDALADWL
                     LSCPLLHENDACVMAHAGIYPLWTLEQARSCAREVEAVLRDDARRDLFFAHMYGNEPS
                     RWDESLQGPERWRTITNFFTRMRLLDAEGHIDLLHKESLTTVPPHLHPWYAWPGRTHI
                     AKKILFGHWAALEGRTGQDHIIALDTGCVWGNRLSAYCLETGIWQACDCHHHRR"
     gene            1543..>2180
                     /locus_tag="VFX11_17195"
     CDS             1543..>2180
                     /locus_tag="VFX11_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX4940412.1"
                     /translation="MSIFSHYQSRYETTQEEEYSLQEYLDICKRDPSAYSSASERMLK
                     AIGEPELVDTSMDPRLSRIFSNKIMRRYPAFDEFYGMEECIEQIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKHLIEKIPFYAIKGSPVNESSLALFSPDEDGAILEEE
                     YGIPKRYLKGIMSPWAVKRLHEFNGDISKFKVVKLYPSILDQIAVAKTEPGD"
BASE COUNT          499 a          672 c          586 g          423 t
ORIGIN      
        1 cataagaatt ccagcgatat tgccgtggaa gtgatcagcg aatacatccc cgaccagtcc
       61 aacgctgccg aacaccgctt tgtcttcgcc tatcatgtcc gcatcgaaaa caggggcaac
      121 ctgccggcgc aactgctgtc ccgccactgg atcatcaccg acggcaacca gcgggtgcag
      181 gaagtgcgcg gcgagggcgt gatcggcgag caacccacga ttctgccagg ggaaacctac
      241 cagtacagca gcggtgccgt gctgcagacg ccggtgggca gcatgaaggg gtcgtatcag
      301 atggtggacg ccaccggcat ccgcttcgac gcgccgattc ccatcttcac cctcgcctca
      361 cccacggccc tgcactgatg gccacctggg caatcggcga tctgcagggc tgttacgagg
      421 atttccgctg cctgctggag aaaatccgct tcaacgccga ccgcgaccag ctctggctca
      481 ccggcgacct ggtgaaccgg ggccccgcct cgctggacac cctgcgtttc tgccacgaac
      541 gccgcgacaa cctgaccacc gtgctgggca accacgacct gcacatgctg gcggtgttgt
      601 ctggcagcat cgaattcaag cgcaaggata cgttccgcga cgtggtgaag gcatctgatc
      661 gcgacgccct ggcagattgg ctgctgagct gcccgctgct gcatgagaac gacgcctgcg
      721 tgatggccca cgccggcatc tacccgctgt ggaccctgga acaggcccgc agctgcgccc
      781 gcgaagtgga agccgtgctg cgcgacgacg cccgccgcga cctgtttttt gcccacatgt
      841 atggcaacga gcccagccgc tgggacgaat cactgcaggg gcccgagcgc tggcgcacca
      901 tcaccaactt cttcacccgc atgcgcctgc tggatgcgga aggtcatatc gacctgctgc
      961 acaaggaatc cctcaccacc gtgccgcctc atctgcatcc ctggtatgcc tggccggggc
     1021 ggacccacat cgcaaaaaaa atcctgttcg ggcactgggc cgcgctggaa ggccgaaccg
     1081 gccaggacca catcattgcg ctggacaccg gctgcgtgtg gggcaaccgg ctcagcgcct
     1141 actgtctgga aaccggcatt tggcaggcgt gcgactgcca ccaccaccgc cgctaatcgc
     1201 cattcaatcg caacatccgc ccgcctgata acccgcgact ataacggctg gaataaacag
     1261 ccgttttaaa attctttgca gctttcgctt cccatttcgc tccaaaaatt ctaaaaatag
     1321 aaaaaagggc aataaatcct ttcgattcaa ctgttttttg gtttttgttt ttggcgtttg
     1381 atactaagct tcaaacaggg cccgattttg cggacaacac ttggtcacta aaccgttact
     1441 atttttggtt gtccattttg atgcgctttc gtattgtgaa aacagcgaga gccccgattg
     1501 caagaccact gacgcgcaca cgccggaaca cgaggtagat tcatgagcat cttcagtcac
     1561 taccagagcc gctacgaaac cacccaggaa gaagagtatt cactgcagga atacctcgac
     1621 atctgcaaac gcgatccgtc ggcgtactcc agcgcatccg aacgcatgct gaaagccatc
     1681 ggcgaacccg aactggtcga cacgtcgatg gacccgcgcc tgagccggat attttccaac
     1741 aagatcatgc gccgttaccc cgcgttcgac gaattctatg gcatggagga atgcatcgag
     1801 cagatcgtgt cgtacttccg ccacgccgcg cagggactgg aagaaaagaa acagatcctg
     1861 tatctgctgg gtccggtggg tggtggtaaa tccagtctgg cggaaaaact caaacacctg
     1921 atcgaaaaaa ttccgttcta cgcgatcaag ggttcaccgg tgaacgaatc atcgttggcg
     1981 ctgttctcgc ccgatgaaga cggcgcgatt ctggaagagg aatatggcat tccaaaacgc
     2041 tatctgaaag gcatcatgtc gccatgggca gtaaaacgcc tgcacgaatt caacggcgac
     2101 atttccaaat tcaaggtagt gaaactgtat ccttctattc tggaccagat cgccgtggcc
     2161 aagaccgagc cgggcgacga
//
LOCUS       DASUEG010000909         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_685215, whole genome shotgun sequence.
ACCESSION   DASUEG010000909 DASUEG010000000
VERSION     DASUEG010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685215"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..1280
                     /locus_tag="VFX11_17200"
     CDS             519..1280
                     /locus_tag="VFX11_17200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4940413.1"
                     /translation="MRMNKSLLGLSMWVACLFADVASAGVVSIQQYSGFLYSGSLGTT
                     SLEHTGINYSEDQLSPAGVTASFTSNLDADNLGSVTWSFANNSGSLLADAWFFVFLDA
                     EIDQATNTFFNESGALASVNGMGAGDIFADSWEIDEPGYLFGDIYDHLLAGYLDNSNG
                     VPAGAEDDVSMALGFSLGDLEAGAIVTAEMIFSRFNIGGLNHVDDDSAESLFFNGTAV
                     VQGTAPVQVPEPGIAVILLTGLLFVGLARRKSRLS"
     gene            1336..>3859
                     /locus_tag="VFX11_17205"
     CDS             1336..>3859
                     /locus_tag="VFX11_17205"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX4940414.1"
                     /translation="MPSKKLLRSLLPAPLAAAFLSTFVSVATTAQAALGPVAADVLVV
                     IDESGSMSGEQRWVADVVPALEQNLVSYGVGSEGQSNLYGLIGFGDSRVVPRSLLLGG
                     KLLGSPAEFVQASSGLRVNGGTEDGWRGIEFALDTYPRRSGAAVNIILATDEDRDNTN
                     SAITFNTVLAKLQANKALLNAVVNATLRCGNNTPALGMDSTGVGYVADGSGGFTTCTG
                     ARAISGSGTTVANYVNLAVQSGGAVWDLNFLRSGGHYARSFTNALLSIKVDEILDQRP
                     VSDLVAVAQAVPNPAAVSQPVILDGAKSFHQREGRTIVSWEWDLNNDGVYDATGPQVT
                     TTFPAIGQYPVTLRVTDDSATPLVDSATLTVNVATGALKPTAVAGGPYLFCPQDTHWW
                     LDGSRSVNPDDGQSAAGGVPDRITEYAWDLNNDLNFSDRTGATINAAAQLQALGVGDH
                     VVRLRVTDNNAAAFPGPGQTNLSDTSITQVSVRDASDLLCNCLPDLAARPKNTKVQLT
                     WTDTGAYQYAVYRSLQNGGPYQQIAVTDSRYSTFLDLGLQLDTPYYYVVSALGRNGAE
                     ICRSRQISATPTARRLDPANRAPVISSVPVTAATEGTPYRYDVNATDPDTRDTVTYSL
                     SVAPVGMTIDAVSGLISWTPVNAQVGQHAVTVQASDIQGAFTNQSFVITVANSNQQPA
                     IVSQPITTATENVAYRYLVQAVDPDLGDQLTFALVSAPAGMIINSQSGEISWTPTGSQ
                     VGNQAVEVQVADAAGAIALQTFVVVVAGQNVAPSITSTPVVVASTGQEYRYDVDAADP
                     DAGDTLTFALGAFPDGMVIDTATGLIAWTPTPAQVGTHAITVV"
BASE COUNT          708 a         1120 c         1180 g          851 t
ORIGIN      
        1 ctggtgaatg acgggccggt gacgttctgg ctggagagtt gaaccaggcg cttgccgatg
       61 ctggcattgt gaatcgtcaa gcccctgaat gtacgcgctg tatcctcgct gcgaataccg
      121 gttccttaac aaaaggttca cgcgctgctg ttatttttgc acagcgattt tgtgcgaaat
      181 ttggcgtggc ctctgtatac cccttttacg gccgttgagg cgtggcattc agtcgttata
      241 atgccgccgg ggatttttca cagggctgtg ccgcattggg gatttttcga tgcacggacg
      301 ttgggcgctc atcacgcagc gacttttcat tttctcgtcc aatgggaagc ctgccagctt
      361 ttcgctgacg gcaggttgtg tcccgggccg gcgtttcctt cacgtcgccc ttcgttctgt
      421 ttcctgttcc cggatttcat tgcctgtttc ttgcggtaat cgcgccagca acgaggcttc
      481 ttgttttcgt ttttgattaa ccaaatgcag ggctttttat gcgtatgaac aaatctctat
      541 tggggctttc aatgtgggtg gcatgcctgt ttgctgacgt agccagcgct ggcgtggtct
      601 cgatccagca gtacagcggg tttctctatt ccggttcgct gggaacgacg tcgctggaac
      661 ataccggcat caactacagc gaggaccagc tgtcgcccgc cggtgtcacg gcttccttca
      721 ccagcaatct ggatgcggac aatctgggtt ccgttacctg gtcgtttgcc aacaactcag
      781 gcagcctgct ggcggacgcc tggttttttg ttttcctcga tgccgaaata gaccaggcga
      841 ccaacacttt tttcaatgag tcgggtgcgc tggcctccgt caacggtatg ggcgcaggcg
      901 acatcttcgc cgacagctgg gaaatcgacg aacccggtta cctgtttggc gatatctacg
      961 accacctgct tgccggttac ctggataaca gcaatggcgt gcccgcggga gcggaagacg
     1021 atgtgtccat ggcgctgggt ttcagcctgg gggatctgga agcgggcgcc attgtcaccg
     1081 ccgagatgat cttcagccgt ttcaatatcg gtggcctgaa tcatgtggat gacgattccg
     1141 ccgagtcgct gttcttcaat ggaaccgcgg tggttcaggg tactgcgccg gtacaggtgc
     1201 cggagccggg gattgccgtc atcctgctga ccggattgct gttcgtgggc ctggcccgca
     1261 gaaaaagccg cctgtcataa cgacgccaga aacgacactt atcttttttc agggaccgat
     1321 tctttgggga cgatcatgcc tagcaaaaaa ttgctgcgtt cactgctgcc agcgccgctg
     1381 gcggctgctt tcctttccac gtttgtatcc gtggcaacaa ccgcccaggc agcgctggga
     1441 ccggtggctg ccgatgtgct ggtggtcatc gacgagtcag gctccatgag tggagaacag
     1501 cgctgggttg ccgatgtggt gccggcgctg gagcagaatc tggtgtccta tggtgtcggc
     1561 agcgaggggc agtcgaacct gtacggtctg atcggatttg gcgacagtcg ggtggtgccc
     1621 cgcagcctgc tgctgggtgg caaattgctg ggcagtcctg ccgagttcgt gcaggccagc
     1681 agcgggttgc gggtcaatgg tggaacggaa gatggctggc gcggcattga atttgcgctg
     1741 gatacctatc cccgtcgcag tggcgcggca gtgaatatca ttctggccac cgatgaggat
     1801 cgcgacaaca ccaacagcgc gatcacgttc aataccgtgc tggccaagct gcaggccaac
     1861 aaggcgttgc tcaatgcggt ggtcaacgcc accctgcggt gcggcaacaa cacaccggcg
     1921 ctgggcatgg acagtaccgg cgttggctat gtggccgatg gcagcggcgg tttcactacc
     1981 tgcaccggcg cccgcgcgat cagtggttcc ggtaccacgg ttgccaatta tgtcaatctg
     2041 gcggtgcaga gcggtggtgc ggtgtgggac ctgaatttcc tgcgctccgg cggacattac
     2101 gcccgctcgt tcaccaatgc gctgctcagc atcaaggttg acgaaattct tgaccagcgc
     2161 cccgtcagcg acctggttgc cgttgcgcag gcggttccca atcccgccgc ggtgagccag
     2221 ccggtgatac tggacggcgc caagtccttc catcagaggg aaggcagaac catcgtttcc
     2281 tgggaatggg atttgaataa cgatggcgtg tacgatgcga ccggtccgca ggtgacaacc
     2341 acattccctg ccatcggcca gtatccggtt acgctgcggg tgaccgacga cagcgcgacg
     2401 ccgctggtgg acagtgcgac cttgacagtc aacgttgcga ccggtgcgct caagccgaca
     2461 gcggtagccg gtggacccta tctgttctgc ccgcaggaca cgcactggtg gctggatggc
     2521 agccgttcgg tcaaccccga tgacggccag agtgccgccg gtggcgtgcc tgaccgcatc
     2581 accgaatatg cgtgggacct gaacaacgat ctgaattttt ctgaccgcac tggcgcaacg
     2641 atcaacgctg ccgcccagct gcaggcactt ggcgtgggtg atcatgtagt gcgcctgcgg
     2701 gtgacggaca acaatgccgc tgcattcccc ggccctggcc agaccaatct ttccgatacg
     2761 tccatcaccc aggtgagtgt gcgtgatgcg tcggacctgc tgtgcaactg tctgccggat
     2821 ctggcggcgc ggccgaagaa caccaaggta cagctgacct ggaccgacac cggcgcctat
     2881 cagtatgccg tgtatcgcag cctgcagaac ggtggtccct accagcagat tgctgtcact
     2941 gacagccgct attccacctt cctcgatctg ggtctgcaac tggacactcc ctactattac
     3001 gtggtgagcg cgctgggtcg caatggtgcc gagatctgcc gttcgcgcca gatttccgca
     3061 acacccactg cgcggcgtct ggaccccgcc aaccgggcac cggttatttc ctccgtccct
     3121 gttacggcgg ccactgaagg caccccatac cggtacgacg tgaatgccac cgatccggat
     3181 acccgtgaca cagtcaccta cagtctgtcc gtggcgccgg tgggcatgac catcgatgct
     3241 gtcagcggtc tcatcagctg gacaccggtc aatgcgcagg tgggtcagca cgctgtgacc
     3301 gtacaggcgt cggatatcca gggcgctttc accaaccagt cgtttgtgat cacggttgcc
     3361 aacagcaacc agcagcccgc catcgtcagt caaccaatca ccacggcaac cgaaaatgtc
     3421 gcttaccgtt atctggttca ggccgttgat cctgatctgg gcgatcagct gacgttcgcg
     3481 ctggtgtcag ctccagccgg catgatcatc aacagccaga gcggcgaaat cagctggaca
     3541 cccaccggtt cgcaagtggg caatcaggca gtggaggtgc aggtggcgga tgccgccggt
     3601 gcgattgcgt tgcagacttt tgtcgtggtg gtggcgggcc agaatgttgc gccgtccatt
     3661 accagcacac cggtggttgt ggccagtaca ggccaggagt accgctacga tgtggatgcg
     3721 gctgatcccg atgcgggcga tacgctgaca tttgcactgg gtgcattccc cgacggcatg
     3781 gtgatcgaca ctgcgaccgg cctgattgcc tggacaccaa cgccggcaca ggtgggcaca
     3841 catgcgatca cggtggtgg
//
LOCUS       DASUEG010000910         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2111533, whole genome shotgun sequence.
ACCESSION   DASUEG010000910 DASUEG010000000
VERSION     DASUEG010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111533"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /gene="thrH"
                     /locus_tag="VFX11_17210"
     CDS             <1..485
                     /gene="thrH"
                     /locus_tag="VFX11_17210"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEX4940415.1"
                     /translation="RLRILDEHGLGLNEIQEVIADMGPMDGALEFVDWLRERFQVVIL
                     SDTFYEFAQPLMRQLRWPTLMCHKLEVAPNGRIVDYKLRQKDPKRQAVKAFHALNYRC
                     IAAGDSYNDTTMLGEAEVGILFKAPQNVINEFPQFPSVTTYEDLKKEFIKGSIRELSL
                     "
     gene            complement(544..1692)
                     /gene="pabB"
                     /locus_tag="VFX11_17215"
     CDS             complement(544..1692)
                     /gene="pabB"
                     /locus_tag="VFX11_17215"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX4940416.1"
                     /translation="MQVSITAMIDQLALFGIGDGGAGPLPGWYGLASYDLYGALHGIE
                     QPAPTLALDRLQAGFYPSVVVIDHQERQCNVVTLQGHEQHAEELLDALLHQTIDAGAA
                     PFRLTTPFDSNLSRDAYLRRFDQVQAYLHAGDCYQVNLAQRFSARCSGDPWDAFNRLT
                     SALAAPMASYFAGAGFHCLSLSPERFLSIRNNHITTHPIKGTRPRHPDPVQDQANAEA
                     LQNSEKDRAENLMIVDLLRNDLGRCCVPGSIQVTELFAVESFANVHHLVSTVEGDLRA
                     GIHPLQAFFSAFPGGSITGAPKRRAIEIIAELEPDNRSFYCGCAFYCDVGGKLDSSIL
                     IRSLVQHEGLIHCWGGGGIVADSVGEQEYQETLDKVGIILRTLSATNS"
     gene            complement(1934..2752)
                     /locus_tag="VFX11_17220"
     CDS             complement(1934..2752)
                     /locus_tag="VFX11_17220"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX4940417.1"
                     /translation="MKRTVFFISDGTGITAETLGHSLLAQFDSIDFEQITIPYVNNET
                     RAQDAVSRINKAAAADNAPPIVFDTIVNEDVRRVLAKSNGFMIDIFSSFLKPLETALG
                     ASSSYSVGKSHSIVNNQSYDLRIDSVHFALDNDDGARTRHYDLADVILVGVSRSGKTP
                     TCLYLALQFGVKAANYPLTEDDLDDLDLPKALKDHRSKLFGLSIEPERLAAIRTERKP
                     NSKYASIQQCESEVRGAEAIFNRYGIPFINSTHASIEEISTMIIEAAGIKRRLA"
     gene            3030..>3180
                     /locus_tag="VFX11_17225"
     CDS             3030..>3180
                     /locus_tag="VFX11_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX4940418.1"
                     /translation="MNDYVIWFQDLSKHDIDKVGGKNASLGEMISNLASKGVSVPGGF
                     ATTAHA"
BASE COUNT          737 a          850 c          920 g          673 t
ORIGIN      
        1 agcggctgcg catcctggac gagcacggcc tgggcctcaa cgagatccag gaagtgatcg
       61 ccgacatggg tccgatggac ggggcgctgg agttcgttga ctggctgcgg gaacgcttcc
      121 aggtggtgat tctctccgat accttctatg agtttgccca gccactgatg cgccaactgc
      181 gctggcccac cctgatgtgc cacaaactgg aagttgcacc caacggccgc atcgtggact
      241 acaaactgcg ccagaaagat cctaagcgtc aggcagtgaa ggcattccac gccctgaact
      301 accgctgcat cgccgcgggc gattcctata atgacaccac catgctaggt gaggctgagg
      361 tcggcatcct gttcaaggcg cctcagaacg tgatcaacga gtttccccag tttccgtctg
      421 tgaccaccta tgaagacctg aagaaggaat tcatcaaggg atcgattcgc gagctttctc
      481 tgtaaaggca tcacataaat cgtttacaaa cagacagttg atgctgaatt ctgacaattc
      541 acattaactg tttgttgcgc tcaatgtccg cagaatgatc cccaccttgt ccagcgtttc
      601 ctgatattcc tgttccccca ctgaatccgc cacgataccg ccgccgcccc agcagtgaat
      661 caatccttcg tgctgcacca gcgagcggat gaggatgctg gaatccagct tgccgcccac
      721 atcgcaatag aaagcacaac cgcagtaaaa actgcgattg tccggttcca gctcggcgat
      781 gatttcgatg gcgcggcgct tgggggcgcc cgtgatggaa ccaccgggaa aggcgctgaa
      841 gaaagcctgc aatgggtgga tgccggcgcg cagatcgcct tcgacagtac tcaccaggtg
      901 atgcacgttg gcgaacgatt ccactgcgaa cagttctgtg acctggatgc tgccaggcac
      961 gcaacaacgg ccaagatcat tgcgcaacag gtccacgatc atcaggttct ccgcacggtc
     1021 tttctcgctg ttttgcaggg cttctgcatt ggcctgatcc tgaacgggat caggatggcg
     1081 ggggcgggtg cccttgatgg gatgagtggt gatgtggttg ttcctgatgg agagaaaccg
     1141 ttccggcgac agactcaggc agtggaaacc ggcgccagcg aaatagctgg ccatgggggc
     1201 cgccagtgca gaagtaagcc ggttaaaggc atcccaggga tcgccgctgc agcgggctga
     1261 aaatcgctgt gccagattca cctgatagca gtcaccggcg tgcaggtaag cctgcacctg
     1321 gtcaaaccgc cgcaggtatg catcgcggga aaggttggag tcaaaagggg tggtcaggcg
     1381 aaagggcgcg gcaccagcat caatggtttg atgcagcagt gcatcgagca attcctctgc
     1441 atgctgttca tggccttgca aggtcaccac attacattgt ctttcctggt gatcaattac
     1501 gacaaccgag ggataaaaac ctgcctgcag acgatccagc gccaacgtgg gggccggctg
     1561 ctcaatgcca tgcaaggcgc catacagatc ataactggcc agcccatacc agccggggag
     1621 cggacccgcc ccgccgtccc cgatgccgaa cagtgccagc tggtcaatca tggcagtgat
     1681 cgagacctgc actccggcat ccgttgccgc agccgaccaa ggcagcctga cctgctgcgc
     1741 cggcagcgca gagaacacat caaaccgcgc accctcaaat ccggggcagc tcagaagcag
     1801 gaagtggccg ggtaaatggc gcagtcgcga gaacagcaac ctgctgtccc cgggataagg
     1861 cagtatcaag cgggaaaggg cgggcatctt tttcttgaaa tgacgttaaa tagatgcgat
     1921 aacaatatga ttatcaagca agtctgcgct tgatgccggc tgcttcgata atcatggtcg
     1981 atatttcttc gatcgaagca tgggtgctgt tgatgaacgg aataccataa cgattgaaga
     2041 tggcttccgc accacgcact tcgctttcgc attgctggat cgaggcgtac ttgctgttgg
     2101 gtttgcgctc ggtgcgaatg gcggccaggc gctcgggctc gatactaagg ccaaacagct
     2161 tgctgcggtg atccttgagc gccttgggca gatccaggtc gtccagatca tcttcagtca
     2221 gcgggtagtt tgcagccttg accccgaatt gcagcgccag gtacaggcaa gtaggcgttt
     2281 tcccgctgcg ggaaacaccc accagaatca cgtcggccag atcatagtgg cgggtgcggg
     2341 caccgtcgtc attgtccagc gcgaagtgca cggaatcgat ccgcagatca taactctggt
     2401 tgtttacgat cgagtgcgat ttgcccacag agtaggaaga ggatgcgcca agtgcggtct
     2461 ccagcggctt gaggaagctg gagaaaatat cgatcatgaa gccgttgctt ttggccagta
     2521 cccgacgcac gtcttcattg acaatggtgt cgaatacgat cggtggggca ttgtcggcgg
     2581 cagctgcctt gttgatgcgg gaaacggcat cctgcgcccg ggtttcgtta ttgacgtaag
     2641 ggatggtgat ctgctcgaag tctatactgt cgaattgcgc cagaagcgaa tgaccgaggg
     2701 tttccgccgt gatgccggta ccgtcggaga tgaagaaaac tgtgcgcttc atggctaaat
     2761 cctgattggc aaaaggatcg gaataattta acgtgaaaac ccatttataa cgagtaattt
     2821 attgactttt ttagtagaat cttgcgttct gcgttacata gccgcgtggc ggaaaacggg
     2881 aaggggacac cccctgacta ccgttattcc catgaataac tgtggtgaac gtctggtcac
     2941 gcaaagcggt cggcactgat tgctgctgac ctgaggccgg gcaccaaaag tgccttgatt
     3001 ttcattttta caaggtaggg agaacgacct tgaacgatta cgtaatctgg ttccaggatc
     3061 tttcaaaaca cgatatcgac aaggtgggcg gcaagaacgc ttcactgggc gagatgatca
     3121 gcaatctggc cagcaagggt gtttcggtgc cgggcggatt tgccaccacc gcacatgcgt
//
LOCUS       DASUEG010000911         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1794309, whole genome shotgun sequence.
ACCESSION   DASUEG010000911 DASUEG010000000
VERSION     DASUEG010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794309"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFX11_17230"
     CDS             <1..296
                     /locus_tag="VFX11_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940419.1"
                     /translation="CIGWIFFRAESLQDALNIIVALHVPDFSIAAAPYSGAILGMVVF
                     AILLAHIVDYGIIRYRHALESSRFWFWLPVLVLGQIFVFLIGEPGYDFIYFQF"
     gene            307..1590
                     /locus_tag="VFX11_17235"
     CDS             307..1590
                     /locus_tag="VFX11_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940420.1"
                     /translation="MPLSNGRAGLLMILIALLVLGPAVLFVRGFTGLAEKSGGDVFGI
                     QRIRSVHAQMDYMAQQAGPTVLVFGSSLTNEGFSPRHFDRQAEAMLGTDITSFNIGMG
                     NMKPSYQLLLAKRLREAHERAGRKAALSIIELTPFLLTEKRNAFRPFMTEQVTAVLMS
                     REELMQELWRDPERFARLFTIKYLRDGISAEAITGGLRFLIGSAQSQVPLIASLGQDQ
                     IALITEQRRLQGELNRQIGQQHPDTRKSIVWNPATQGGLIDMEDLSADTQDLARQLVR
                     MMRDPGTLQRDARDREECCDIRHLVFDASMVDEFVTIVEEFKQFSERVEIVLMPTNHT
                     LIAPTPEALARQQQVLQQIAARTGVPLLDYQQHPQFAADDFYDATHLSLDSGKPRFSA
                     LLARDLADELVSVIGANGKTVAGKTVVTGSENGGG"
     gene            complement(1685..>2719)
                     /locus_tag="VFX11_17240"
     CDS             complement(1685..>2719)
                     /locus_tag="VFX11_17240"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEX4940421.1"
                     /translation="AEETPSPLMHSIVEQLCQEMEMEHEQLCSVLVNLLQADLPLEPK
                     ETVREHDERGASYREGRGERESRREREYDETPIEHKPVPLKEFPDIEMERFRIEVGKK
                     HGAKPGDIVGAIANELELESSYIGEINMRGEYSTVDLPEGMPKELLGQLKTIRVRQQQ
                     LRATRYGKEEPGKGNKAFAPQEDARTGKKKFDKPRRERPARDASDHAAPSAQADTAPT
                     LDPIESLLETLTRARPVAEKTAVTDDAATKLSRRSSADKPAQKISYKEKSAGEKPFRE
                     KSFKAGAARSGDAKESGLKDRKFKDKPSKDKPFKEKSFKDKPFKGKTEKGKTFADKTP
                     FKGKPKPKKS"
BASE COUNT          433 a          778 c          824 g          684 t
ORIGIN      
        1 tctgcattgg ctggattttc tttcgtgcag aatcgctgca ggatgcgctg aacatcatcg
       61 tggcgctgca tgtgccggat ttttccattg cagcggcacc ctattcggga gcgattctgg
      121 gtatggtggt gtttgcaata ctgctggcgc atatcgttga ttacgggatc atccgctacc
      181 gccacgcgct ggaatcgtcg cggttctggt tctggctgcc cgtgttggtg ctcgggcaga
      241 ttttcgtatt cctgattggt gagcctggct atgacttcat ctacttccag ttctgagcat
      301 gcctccatgc ccttgagcaa cggccgcgcc ggtctgctga tgatactgat tgcgctgctg
      361 gtgctggggc ctgcggtcct gtttgtgcgc ggctttaccg ggcttgcgga aaaaagtggc
      421 ggcgatgttt ttggcatcca gcgtatccgc tcggtgcatg cgcaaatgga ctacatggcg
      481 caacaggccg gccccacggt gctggtgttc ggttcctcgc tgaccaacga aggtttttcg
      541 ccacgccatt tcgatcggca ggcggaggcc atgctgggaa cggatatcac cagcttcaat
      601 attggcatgg gtaacatgaa gccttcctat cagctgttgc tggccaagcg tctgcgtgaa
      661 gctcacgagc gggccggccg caaggctgcg ctgtcaatca tcgagctgac cccgtttctg
      721 ctcacggaaa agcgcaatgc cttccgcccc ttcatgaccg agcaggtgac agcagtgttg
      781 atgtctcgcg aagaactgat gcaggaactc tggcgtgatc cggagcgttt cgctcgcctg
      841 tttaccatta aatacctgcg cgatggcatt tcagctgagg ccattaccgg cgggctccgc
      901 ttcctcatcg gttcggcgca gtcgcaggtg ccgctgattg ccagcctggg tcaagaccag
      961 atcgcgctga ttaccgagca gcgccggctg cagggagagc tgaaccggca gatcggccag
     1021 cagcatccgg acacgcgcaa atcgatcgtg tggaaccccg ccacccaggg cggcctgatc
     1081 gacatggagg atctgtctgc cgacacccag gacctggcgc ggcagctggt ccgcatgatg
     1141 cgcgatccgg gcaccctgca acgggatgcg cgggatcgcg aggagtgctg cgatatccgc
     1201 catctggtat ttgatgccag catggtggat gaatttgtca ccatcgtgga agaattcaag
     1261 cagttttctg agcgggtgga aatcgtgttg atgcccacca atcacacatt gatcgccccg
     1321 acaccggaag cgctggcgcg gcagcagcag gtgctgcaac agattgcggc ccgcaccggt
     1381 gtgccgttgc tggattacca gcagcatccg caatttgcgg cagacgattt ctacgacgcc
     1441 acacacctgt cgctggactc cggcaagccg cgtttctcgg cgctgttggc acgtgatctg
     1501 gcggatgagc tggtgagtgt aatcggggcc aatgggaaaa ccgtggccgg caagactgtg
     1561 gtcacgggct cggaaaacgg aggcggttag acgccggtcc tttttttgcg caatcttttt
     1621 aaaccggtcg gccgcctgat tttcgaagtg caaaaaaaag ccccgcaatg gcggggcttt
     1681 cagatcacga cttcttcggc ttgggtttgc ctttgaaggg cgtcttgtcg gcgaaggtct
     1741 ttcccttctc cgtcttgccc ttgaacggct tgtccttgaa agacttctct ttgaaaggtt
     1801 tgtctttcga cggtttgtcc ttgaatttcc tgtccttcaa tcccgattct ttcgcgtcgc
     1861 cactgcgggc tgctccggcc ttgaacgatt tctccctgaa aggcttttcc ccggcagact
     1921 tctctttgta cgagattttc tgcgctggct tgtcggcgct ggagcgcctt gagagttttg
     1981 ttgccgcgtc gtccgttact gctgtctttt ccgctaccgg gcgggcgcgg gtgagcgttt
     2041 ccagcaggct ttcgatcggg tccagagtcg gggcggtatc ggcctgcgcg gatggtgccg
     2101 cgtggtcgga tgcgtcccgt gccggtcttt cgcggcgggg tttgtcgaat ttctttttac
     2161 ctgttctggc gtcttcctgc ggcgcgaaag ccttgttgcc cttgccgggc tcttccttgc
     2221 cgtagcgggt ggcgcgcagc tgctgctggc gcacacggat ggttttcagc tggcccagca
     2281 attccttcgg catgccttcc ggcaggtcca cggtggagta ttcaccgcgc atgttgattt
     2341 cgccgatgta gctgctttcc agttccagtt cgttggcgat ggcgccgacg atgtcaccgg
     2401 gtttggcgcc gtgtttcttg cccacctcga tgcggaaacg ttccatttcg atgtcgggaa
     2461 attccttcag cggcactggc ttgtgttcga ttggcgtttc gtcgtattcg cgctcgcgac
     2521 ggctttcccg ttcgccgcgg ccttcgcgat aggatgcccc gcgctcatca tgttcacgca
     2581 cggtttcctt gggctccagc ggcaggtctg cctgcagcag gttgaccagt accgagcaca
     2641 gctgctcgtg ctccatttcc atttcctggc acaactgctc gacgatggag tgcatcagcg
     2701 gcgatggcgt ttcttccgc
//
LOCUS       DASUEG010000912         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_57456, whole genome shotgun sequence.
ACCESSION   DASUEG010000912 DASUEG010000000
VERSION     DASUEG010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57456"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFX11_17245"
     CDS             complement(<1..937)
                     /locus_tag="VFX11_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940422.1"
                     /translation="MPQYKAPLRDMQFLIHEVFNAGQHYQQLGYTDVNLDLIDAILTE
                     GARFTENELAPLRTLGDEHGAKWKDGVVTTPPGFKDAFNAFRDNGWATLAIDPAWGGQ
                     GLPHSLNIIFHEMCFTANVAWGAIVTLTQGAIRAIEAHASDELKALYLPRLGSIEWTG
                     TMCLTEPHAGSDVGLVKTKAEPQADGTYAISGTKIFITQGENDYADNIVHLVLARLPG
                     APKGPKGISLFLVPKFLPDASGQPGARNRVSCASIEKKMGLKGSPTCVLNFDGAQGWL
                     VGQPGAGLACMFTMMNAARLDVGIEGLAAAELSFQG"
     gene            complement(1148..1885)
                     /locus_tag="VFX11_17250"
     CDS             complement(1148..1885)
                     /locus_tag="VFX11_17250"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX4940423.1"
                     /translation="MKPALQLAVLLAAVNSTALLAEETPADKLWGGEVTLNYSQSSGN
                     SNATSFSGKSKAERDGEFWRNTYKLDGDNETAENDEGDETRTAEKYFASARADYKVTS
                     NSFLFGLLEHTDDRFSGYDFETAVSFGYGRQLIETGHHNLKADIGPGYRYSEFEDTGL
                     EEEEAMVRIGAQYVWTINDNAVFDEDFSTEVGEEKTISKSLSRLKVKINGSLWGSVSY
                     EIKHTDEVPPGIKNSDRKTLFGLNYVF"
     gene            complement(1958..>2614)
                     /locus_tag="VFX11_17255"
     CDS             complement(1958..>2614)
                     /locus_tag="VFX11_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4940424.1"
                     /translation="HSTDDDLDDGLVNVGVAYAFGGKKEETKAAPAAVVAAAPADSDG
                     DGVIDPNDKCPGTLAGVKVDATGCELDSDADGVVDSKDQCPGTPAGTAVDETGCRFKK
                     ESVELQIQFPTNSAAIAEQYQSEIKRLADFLVKYPAIAINIEGHTDAQGSNEFNKKLS
                     QQRAESVKGALVTRYGINANRVTPIGYGEEKPIATNDTAAGREQNRRVVAVISASYKE
                     "
BASE COUNT          488 a          757 c          787 g          582 t
ORIGIN      
        1 cgccctggaa cgacagctcc gccgccgcca gtccttcaat gcccacatcg agccgcgccg
       61 cgttcatcat ggtgaacatg caggccagac cggcgcccgg ctgccccacc agccagcctt
      121 gcgcaccgtc gaaattcagt acgcaggtgg gcgagccttt caggcccatt ttcttttcga
      181 tggacgcgca ggagacccgg ttgcgcgcac ccggctggcc agatgcgtct ggcaggaatt
      241 tcggcaccag gaacaggctg atgcctttcg gacctttggg ggcgcccggc agtcgggcca
      301 gcaccagatg gacgatgttg tcggcgtaat cgttttcgcc ctgggtgatg aaaatcttgg
      361 tgccgctgat ggcataggtg ccatcagcct ggggttcggc tttggtcttc accagaccca
      421 cgtcactgcc ggcgtgcggc tcggtcaggc acatggtgcc ggtccactcg atcgatccca
      481 gcctgggcag gtaaagggct ttgagttcat ccgaagcatg ggcttcgatg gcacggatgg
      541 cgccctgggt cagggtgacg atagcgcccc aggccacgtt ggcggtgaag cacatctcat
      601 gaaaaatgat gttcaggctg tggggcaggc cctgcccgcc ccacgcggga tcgatggcca
      661 gggtggccca gccgttgtcg cggaaggcgt tgaaggcgtc cttgaaaccc ggcggcgtgg
      721 tgacgacgcc atccttccat ttcgcgccgt gctcgtcgcc cagggtgcgc agcggggcca
      781 gttcattttc ggtgaagcgc gcgccctccg tcagaatggc atcgatcaga tccaggttaa
      841 cgtcggtgta gcccagctgc tggtaatgct gaccggcatt gaacacttcg tgaatcagaa
      901 actgcatgtc acgaaggggg gctttgtact gcggcatggg tacatcctcg caaggtctgt
      961 tgatgacaag ctgatcagct tcggggcgga attattgcca gtgcagcatg gcgaatcaat
     1021 ggatcgggcc agtgacccaa gagtcagtcc gaattgacct ttgaaatgtc cgtttgacta
     1081 gttttggcta taaatgcggc gggctggcca ccggctggca ccaccagccc ggcggtcagg
     1141 agcaggctca gaacacgtaa ttcaggccga acagggtttt gcggtcggag ttcttgatgc
     1201 ccggcgggac ttcgtcggtg tgcttgattt cgtaagacac cgagccccac aggctgccgt
     1261 tgattttgac cttgagccgg gacagggatt ttgaaatggt tttctcctcg ccaacctcgg
     1321 tgctgaaatc ctcgtcgaac acggcattgt cattgatggt ccagacatac tgggcaccga
     1381 tgcgcaccat cgcctcctct tcctccaggc cggtatcctc gaactcgctg tagcgataac
     1441 cagggccaat gtcggccttg agattgtgat gcccggtctc gatcagctga cggccataac
     1501 cgaaggagac agccgtctcg aagtcgtagc cgctgaaccg gtcgtcggta tgctccagca
     1561 ggccgaacag gaaggaattg cttgtgacct tgtaatccgc cctggctgaa gcgaaatatt
     1621 tttcggccgt acgcgtctcg tcgccttcgt cattctcggc ggtttcattg tcgccatcga
     1681 gcttgtaggt gttgcgccag aactcgccat cacgttcagc cttggacttg ccgctgaaac
     1741 tggtggcgtt gctgttgccg gaactttgcg aataattgag ggtgacttca ccgccccaga
     1801 gtttgtcagc gggagtctct tccgccagca gggcggtcga attcacggcg gccaagagaa
     1861 ccgccagttg cagggctggt ttcatttttc ttctccctga aaaaaagaaa aaccctgccg
     1921 acgggagtac cgccggcagg gtttcgatca cagcatatta ctctttgtag ctggcagaga
     1981 tgactgctac cacgcgacgg ttctgctcgc gaccagcggc agtgtcgttg gtggcaatgg
     2041 gcttctcttc gccgtaaccg atcggagtga cacggttggc gttgataccg taacgggtaa
     2101 ccagtgcacc cttcacggat tcagcgcgtt gctgcgacag tttcttgttg aattcgttgg
     2161 aaccctgcgc atcggtgtga ccttcgatat tgatggcaat cgccgggtac ttcacgagga
     2221 agtcagccag acgcttgatt tcagactggt actgctcggc gatggctgca gagttggtgg
     2281 ggaactggat ctgcagttca acgctttctt tcttgaagcg gcaaccggtt tcgtcaacgg
     2341 cagtgccagc cggagtgccg gggcactgat ccttgctgtc aaccacgcca tccgcatcgg
     2401 aatccagttc gcaaccggta gcgtccactt tcacgccagc cagcgtgccg gggcacttgt
     2461 cgttcgggtc gataacgccg tcaccatcgc tgtccgctgg tgcagcggca accactgctg
     2521 caggggcagc cttggtctct tccttcttgc caccgaaagc gtaggcaaca cccacgttca
     2581 ccaggccgtc atccagatcg tcgtctgtgc tgtg
//
LOCUS       DASUEG010000913         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_971801, whole genome shotgun sequence.
ACCESSION   DASUEG010000913 DASUEG010000000
VERSION     DASUEG010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971801"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="frr"
                     /locus_tag="VFX11_17260"
     CDS             <1..500
                     /gene="frr"
                     /locus_tag="VFX11_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013797130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX4940425.1"
                     /translation="ETAFKKVRTGRAHPSILDGVMVPYYGVDTPLKQVANVTVEDART
                     LAISPWEKQLGSAIEKAIMKADLGLNPSSNGDLIRVPMPALTEQTRKEMIKICKAEAE
                     HGRVAIRNIRRDANTMLKDLLKEKEITEDEERRGQEDVQKLTDKYVAEVEEMLKKKEA
                     DLLEV"
     gene            561..1331
                     /gene="uppS"
                     /locus_tag="VFX11_17265"
     CDS             561..1331
                     /gene="uppS"
                     /locus_tag="VFX11_17265"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX4940426.1"
                     /translation="MKVQSSAPDIDSSAPAIPRHVAIIMDGNNRWAKQRGLRGVEGHK
                     QGARAVRATVENCARAGVEVLTVFAFSSENWRRPEEEVNALMSLFLEALTQEVAELHQ
                     NGIRLVFIGDLSAFTPELRRKMDDSVRLTANNQRMTFAVAVNYGGQWDIANACRQLAS
                     QVEQGTLRADQITPEKVQQCISLGDLPMPDLCIRTGGENRISNFMLWQMAYAELYFTD
                     LYWPDFDQAALDAAFADFTRRQRRFGRTSEQIAASQEE"
     gene            1335..2144
                     /locus_tag="VFX11_17270"
     CDS             1335..2144
                     /locus_tag="VFX11_17270"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX4940427.1"
                     /translation="MLKQRIITALILLPLILGAIFYLDTPWFALAMAIPVALGAWEWA
                     NIMGIEDPVRRLPYVGIVLALLMLVYLTRFNAVLYLGCLWWLVAVWLVKSYPAEVVRW
                     EGRGVQSLIGLLVLVPAWWSLVLIQGQADGHWWLLYVMLLVWGADTGAYFVGRKLGKN
                     KLAPAVSPGKSIEGLLGGLATTAVIALLVAFSTHASDRAGLIPFLLISLVAVIGSVFG
                     DLAESMFKRHRGIKDSSQLLPGHGGVLDRIDSITAAVPLFASGMLLLGAMQ"
     gene            2154..>2533
                     /locus_tag="VFX11_17275"
     CDS             2154..>2533
                     /locus_tag="VFX11_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX4940428.1"
                     /translation="MTERKRVTVLGSTGSIGVSTLDVIARHPAEFEVFALTANNRVEP
                     LLQQVLATQPRYAVMRDELSAEQLRSRLRELGSDTEVLAGESGLVTVSSHPQTDYVMA
                     AIVGAAGLMPTLAAVKTGKRVLLA"
BASE COUNT          490 a          730 c          812 g          501 t
ORIGIN      
        1 tggaaacggc attcaagaaa gtcagaaccg gtcgtgccca ccccagcatt ctggacggcg
       61 tgatggtgcc ctattacggc gtggacacac cgctcaagca ggtggccaac gtcaccgtgg
      121 aagatgcgcg caccctggcg atttccccct gggaaaagca gctgggttct gccattgaaa
      181 aagccatcat gaaagccgac ctgggcctga accccagctc caacggtgat ctgatccggg
      241 ttcccatgcc ggcactgacc gagcagaccc gcaaggaaat gatcaagatc tgcaaggcgg
      301 aagccgagca tggtcgtgtc gcgatccgca atatccgccg cgacgccaat accatgctga
      361 aggatctgct gaaggaaaag gaaatcaccg aggacgagga gcgtcgcggc caggaggacg
      421 tacagaagct gacggacaag tacgtcgccg aagtcgaaga aatgctgaag aaaaaagaag
      481 cggatctgct cgaagtctga tccgcccggc ctgcgccatt gcccggcgca ggccgaccga
      541 ttccatcacg gaggataccc ttgaaagtcc agtcttccgc tcccgatatt gattcctccg
      601 ctcccgccat tccccgtcat gttgcgatca tcatggatgg caacaaccgc tgggcgaagc
      661 agcgcggcct gcgcggcgtt gaaggccaca agcagggtgc ccgtgccgtg cgtgccacag
      721 tggagaattg tgcccgggcc ggggttgaag tactcaccgt gtttgcgttc agcagtgaaa
      781 actggcgccg gccggaagag gaggtcaacg ccctgatgag cctgttcctg gaagccctga
      841 cccaggaagt ggctgaactg caccagaacg gcatccggct ggtgttcatc ggtgatctgt
      901 ctgcctttac gcccgagttg cggcgcaaga tggacgattc ggtgcgcctg accgccaata
      961 accagcgcat gacctttgcc gtcgccgtga attatggtgg ccagtgggat atcgccaatg
     1021 cgtgccgtca gctggccagc caggtggagc agggcaccct gcgcgctgat cagatcacgc
     1081 cggaaaaagt gcagcagtgc atcagcctgg gtgatctgcc catgccggat ctgtgcatcc
     1141 gcaccggcgg tgaaaaccgc atcagtaatt tcatgctgtg gcaaatggcc tacgcggaac
     1201 tttactttac cgacctttac tggcctgatt tcgatcaggc ggccctggac gcggcgttcg
     1261 cggatttcac gcggcgccag cgtcgtttcg ggcgcaccag cgagcagatt gcagcgagcc
     1321 aagaagaata agacatgttg aagcagcgca tcattacagc actgattttg ttgcccctga
     1381 tactcggggc aattttttat ttggatacac cctggttcgc cctggcgatg gccattccgg
     1441 tggccctggg cgcctgggaa tgggccaata tcatgggtat cgaagacccg gtccgccgcc
     1501 tgccctatgt gggcatcgtg ctggcactgc ttatgttggt gtatctcacc cgtttcaacg
     1561 cggtccttta tctgggctgc ctgtggtggc tggtggccgt gtggctggtg aaatcctacc
     1621 cggctgaggt ggtgcgctgg gaaggccgcg gtgtccagag cctgatcggc ctgctggtgc
     1681 tggtgccggc ctggtggtcg ctggtcctga ttcaggggca ggctgacggg cactggtggc
     1741 tgctgtatgt gatgttgctg gtgtggggtg ccgataccgg ggcctatttc gtgggtcgca
     1801 agctgggcaa gaacaagctg gcgccggcgg tcagtccggg caagtccatc gaaggcctgc
     1861 tgggcgggct ggccaccacg gccgtcatcg ccctgctggt ggctttttcc acccacgcgt
     1921 ccgaccgcgc cggattgatt cccttcctgc tgatcagtct ggtggccgtg atcggctcgg
     1981 tattcgggga tctggcggag agcatgttca agcgtcatcg cggtatcaag gacagtagcc
     2041 agttgctgcc gggacatggc ggcgtgctgg accgtatcga cagcatcact gccgctgtgc
     2101 ccctgtttgc cagcggcatg ctgttgctgg gagcaatgca atgaggcaat cccatgactg
     2161 aaaggaaacg cgttactgtg ctgggaagca cgggttccat cggcgtcagc acactggatg
     2221 tgattgcccg gcatcccgcc gagtttgaag tatttgccct gaccgccaac aaccgggtgg
     2281 aacccctgct ccagcaggta ctggccacgc aaccgcgcta tgcggtgatg cgggatgaac
     2341 tgtcagcgga gcagttgcgg agccgtctgc gcgagctggg ttccgacacg gaagtgctgg
     2401 caggggagag cggactggtt acggtatcca gccatccgca gaccgactac gtgatggccg
     2461 ccattgtcgg cgccgccggc ctgatgccga cccttgcggc tgtgaaaacc ggcaagcgcg
     2521 tgctgctggc caa
//
LOCUS       DASUEG010000914         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_846712, whole genome shotgun sequence.
ACCESSION   DASUEG010000914 DASUEG010000000
VERSION     DASUEG010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846712"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /gene="csrA"
                     /locus_tag="VFX11_17280"
     CDS             <1..169
                     /gene="csrA"
                     /locus_tag="VFX11_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX4940429.1"
                     /translation="GETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVAVHREEIYQRIQ
                     REKGGEHDTGA"
     gene            306..398
                     /locus_tag="VFX11_17285"
     tRNA            306..398
                     /locus_tag="VFX11_17285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Ser,seq:gct)
     gene            582..658
                     /locus_tag="VFX11_17290"
     tRNA            582..658
                     /locus_tag="VFX11_17290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:616..618,aa:Arg,seq:acg)
     gene            complement(818..1126)
                     /locus_tag="VFX11_17295"
     CDS             complement(818..1126)
                     /locus_tag="VFX11_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940430.1"
                     /translation="MNKAIVLVAVAALGLAACNKQEESKALESAKESAAQAVEAAKDA
                     AGHASDAVQEAGEAAKEVAVEGTQAAVDAAAEEAKKAADKTKETAEASAEAVKEALPQ
                     "
     gene            complement(1281..>2083)
                     /locus_tag="VFX11_17300"
     CDS             complement(1281..>2083)
                     /locus_tag="VFX11_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940431.1"
                     /translation="IFACLFIAGVIYLLWPLLKESATARFWAAGALFSVAPITTTVPA
                     DRNLLFVGIGSSMLLGMLFHTLFTRLQTSRLHRGGTWILVVIHLILAPLLMPVFSYSP
                     QIWNHLMGLPLARKIPITNPDESLLTFGIPMPIGLGSTPMRFAEGLPLANKFWMISSL
                     KQNFTITRANHDTLLVKAEDGFIDSIEATLRNVHRKPFTPDFKISLTDMDIAVTRVDE
                     NGRPLELTLTFNNNRLEKTRILTWTGKTFERHDVPKTAGETLALQLDP"
BASE COUNT          440 a          539 c          630 g          474 t
ORIGIN      
        1 aggcgaaacc ctgatgatcg gcgacgaagt caccgtgacc gtgctgggcg taaagggtaa
       61 tcaggtgcgg atcggtgtga atgcgccgaa ggaagtcgcc gtccaccggg aagagatcta
      121 tcagcgcata cagcgcgaaa aaggcggtga gcacgacact ggcgcctgaa acgcgtccgg
      181 aagtggtccc gatgaaagcg atgcttcggc atcgcttttg tttttaagga cttacgtgcg
      241 atcaaattag ccagaatggg ctaggaattt gcgccaaacg cgggtatcat tctgcccacg
      301 tttttggaga ggtggccgag aggccgaagg cgctcccctg ctaagggagt atacggtcaa
      361 aagctgtatc gagggttcga atccctccct ctccgccata ttcccccggg aatatccgga
      421 gtctgaaagt taaatccccc gatttttcat gatgatgctt cggtcgagcc agagcaggtt
      481 cgcaggttgc aaacggtgtt gatcggttcg gcaggttgag tcaaaaaagt gcaataactg
      541 attgactcgg aagcgcgaat tcgtaaaatg cacactctca cgcgccagta gctcagctgg
      601 atagagtacc tggctacgaa ccaggcggtc ggaggttcga atccttcctg gcgcgccata
      661 ttgaagaaag aaaaaaggct gcagaaatgc agcctttttt tatgcccgtt cgacaggata
      721 tctatttcgc aggattcttc ggtgagatgc ggaccggatg gatctccggc ccgtttccct
      781 ggtttgcaga acgacgtctg cagaaccgca ttgacgctta ctggggcaga gcttctttta
      841 ctgcttcagc ggatgcttcc gcggtttcct tggttttgtc ggcagctttc ttcgcttctt
      901 cggcagcggc gtcaacagcg gcctgagtgc cttccactgc aacttccttg gcggcttcac
      961 cggcttcctg cacggcgtca gatgcgtgac cagcggcatc cttggctgct tcaactgcct
     1021 gggcagccga ttctttcgcg ctttccagtg ctttcgattc ttcctgcttg ttgcacgctg
     1081 ccagacccag tgccgcaact gctaccaata cgattgcctt gttcattgtc gtgcctccag
     1141 tggttcaaag aggcgcgagg ttaagccaat tcgctcgata aattaagttt gattagtaca
     1201 ttttcgacat cggtcacgtt ttcattcgtc ggctggaaac cggcagccgt attgcagcct
     1261 gctgccggat gtgaccagca tcaggggtcc agttgcagcg cgagcgtttc acctgccgtc
     1321 ttcggcacgt catgacgctc gaacgtttta ccggtccagg tcaggatgcg ggtcttctcc
     1381 agccggttgt tgttgaacgt cagggtcagc tccagcgggc ggccattttc atccacgcgt
     1441 gtgacggcaa tatccatgtc ggtaagcgat attttgaaat ccggcgtgaa cggttttctg
     1501 tgcacgttgc gcagcgttgc ttcgatggaa tcgatgaatc cgtcttcggc ctttaccagc
     1561 agggtgtcgt ggttggcccg ggtgatggtg aagttctgtt tgagcgagga aatcatccag
     1621 aacttgtttg ccaatggcag gccttcggca aagcgcatgg gggtcgagcc caggccgatg
     1681 ggcatgggaa tgccgaaagt cagcaggctt tcgtccggat tggtgatcgg aatcttgcgt
     1741 gccagcggca gccccatgag gtgattccag atctgcgggc tgtagctgaa caccggcatc
     1801 agcagagggg ccaggatcag atgaatcaca accagaatcc aggtgccacc acggtgcagg
     1861 cgggacgtct gcaggcgggt gaacagggta tgaaagagca ttcccagcag catggatgag
     1921 ccgatgccga cgaacagcag gttgcggtcg gccggcacgg tggtagtgat gggggcaacc
     1981 gagaacaggg cgccggcggc ccagaagcgg gcagtggcgg attccttcag tagcggccac
     2041 agcagataaa tgacgccggc gatgaacagg caggcgaaga tca
//
LOCUS       DASUEG010000915         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1758084, whole genome shotgun sequence.
ACCESSION   DASUEG010000915 DASUEG010000000
VERSION     DASUEG010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758084"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="clpX"
                     /locus_tag="VFX11_17305"
     CDS             complement(<1..725)
                     /gene="clpX"
                     /locus_tag="VFX11_17305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HEX4940432.1"
                     /translation="MTDDRDGKGEDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECV
                     DLCNDIIREEVQEDSNDKNSDKLPTPIEIKDILDDYVIGQDRAKKVLSVAVYNHYKRL
                     RSGAAKSAKGSNVEVELGKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEA
                     GYVGEDVENIIQKLLQKCDYDVEKAQMGIVYIDEIDKISRKSDNPSITRDVSGEGVQQ
                     ALLKLIEGTVASVPPQGGRKHPQ"
     gene            complement(856..1485)
                     /gene="clpP"
                     /locus_tag="VFX11_17310"
     CDS             complement(856..1485)
                     /gene="clpP"
                     /locus_tag="VFX11_17310"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX4940433.1"
                     /translation="MSHLLNELAERNPILNTGLVPMVIEQTARGERSFDIYSRLLKER
                     VIFMVGQVEDYMANLIVAQLLFLESENPDKDIHLYINSPGGSVTAGMAIYDTMQFIKP
                     DVSTMCVGQACSMGSLLLTAGAQGKRFCLPNSRVMIHQPLGGFQGQASDIEIHAREII
                     QMKRRLNEILAHHTGQPYDTIDRDTDRDNFMSAYDAKKYGLVDEVLEKR"
     gene            complement(1691..2998)
                     /gene="tig"
                     /locus_tag="VFX11_17315"
     CDS             complement(1691..2998)
                     /gene="tig"
                     /locus_tag="VFX11_17315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX4940434.1"
                     /translation="METTSGLGRRMTVGVPADKIDNAVETKIVETAKRVRIDGFRPGK
                     VPVREVRRRYGKAIREEVIGEIVSNSFYEAMNQQALNPAGYPSIERTKDQPGEDFEFV
                     ATFEVYPQVSVQGLSSISVSRPVADITEADIDTMIQRLREQRATFADVERPAQNGDQL
                     IIDFVGTKDGEAFQGGSAENSKLVLGSGSMIPGFEDGLLGATKGEERVLALTFPADYH
                     SEELKGQAVQFAVTVKAVQEKQLPEVNEEFIKLFSPKESSMENFRAEIRQNMERELKN
                     AVKNRVKKQVMDGVLEQNSIDAPKALIDSEIDRQRRQMVQQFGGGANFDFRTLPAELF
                     EQQATRAVKLALLLGELIKERQIKADATRVRAAIEEMAQPYENPAQVVSWYYENEQVL
                     QQVEAMVLEDQVVDVILEQAQLVDETMSYEDAVRPANQDQADD"
BASE COUNT          682 a          886 c          846 g          807 t
ORIGIN      
        1 tgctgcggat gcttgcgtcc accctgcggc ggaaccgaag ctaccgtacc ttcgatcagc
       61 ttcagcaacg cctgctgcac gccctcgccc gatacgtccc gggtaatgga cggattgtca
      121 gatttgcggg aaatcttgtc gatctcatcg atgtagacga tacccatctg ggccttttcc
      181 acatcgtaat cacatttctg cagcaatttc tggataatgt tctcaacgtc ttcaccgaca
      241 tagcccgctt cggtgagcgt cgttgcatcg gcgatggtga atggcacatt caacaaacgg
      301 gccagcgttt ccgccagcag cgttttaccg ctaccggtag gaccaatcag cagaatgttg
      361 ctcttgccca gttcaacttc aacattggag cctttcgcac ttttggccgc accggaacgc
      421 agacgcttgt aatggttgta caccgctacc gacaacactt tcttggcgcg gtcctgtcca
      481 atcacgtagt cgtcgagaat atccttgatt tcgatggggg tcggcagttt gtcgctgttc
      541 ttgtcgttgc tgtcttcctg cacttcttcg cgaatgatgt cgttgcaaag atcgacacat
      601 tcgtcgcaga tgaatacaga gggaccggcg atcagtttgc gcacttcatg ctggcttttc
      661 ccgcaaaatg agcaataaag cagcttgccg ctgtcttcgc cttttccgtc acgatcgtca
      721 gtcatttatt tacctcttct agcctgcatc gggctcttag aaagatgcag cgaaaacccc
      781 gtttttgcaa gtacgagatt cccgatcagt tgacaccgga tctagctata tcttttatgt
      841 gattgttttt taagcttatc ttttttcgag tacttcgtcc accaacccgt atttcttggc
      901 gtcgtaggcg ctcatgaaat tgtcgcggtc ggtgtcgcgg tcaatggtgt cataaggctg
      961 gccggtgtgg tgagccagga tctcgttgag gcgccgtttc atctggatga tttcgcgggc
     1021 atggatctcg atatcagacg cctgaccctg gaagccgccc agcggctggt gaatcatgac
     1081 gcgggagttc ggcaggcaga agcgcttgcc ctgggcgccc gcggtcagca gcagggaacc
     1141 catggaacag gcctgaccca cgcacatggt ggatacgtcg ggcttgatga attgcatggt
     1201 gtcataaatc gccatacccg cagttacgga cccgcccgga gaattgatat agaggtggat
     1261 atccttgtcc gggttttccg attccaggaa cagcagctgt gccacgatca ggttggccat
     1321 gtagtcttcc acctgaccca ccatgaaaat cacccgctct ttcaacaagc gggaataaat
     1381 gtcaaacgaa cgctcgccac gggcagtctg ctcgataacc atgggaacca aaccggtgtt
     1441 caggatgggg ttgcgttcgg cgagctcatt cagaagatgg gacatgggga tgacttcctt
     1501 tagttgatta attgagcaga caagaatcgt tcgcaattca ccctgaggat atgcggtctt
     1561 ttcaagcgct ttcaagacct tgtcgcgttt atttttacta tagccccgat cgggtcagcc
     1621 atcccagtga agcacaaaaa aaaacgcgac ctcaagggtc gcgttctcgt tgataccgga
     1681 ttgccccgta tcaatcgtcc gcctgatcct gattggcggg acgtactgcg tcctcgtagc
     1741 tcatggtctc gtcgacgagc tgggcctgct ccaggatgac atccaccacc tggtcttcca
     1801 gcaccatcgc ttcaacctgc tgcagcacct gttcgttttc atagtaccag ctgaccactt
     1861 gcgccggatt ttcgtaaggc tgtgccattt cttcgatagc agcgcgcacg cgggtggcat
     1921 ctgccttgat ctggcgttcc ttgatcagtt cacccagcaa cagagccagc ttgacagcgc
     1981 gggtggcctg ctgttcgaac aactcggcgg gcagagtgcg gaaatcaaaa ttggcgccgc
     2041 caccaaactg ctgcaccatc tggcgacgct ggcggtcgat ctcggaatca atcagggctt
     2101 tcggcgcgtc aatgctgttc tgttccagca cgccgtccat tacctgcttt ttcacccggt
     2161 tttttaccgc gttcttcagc tcacgctcca tattctgacg gatttcggca cggaaatttt
     2221 ccatgctgga ttccttgggc gagaaaagct tgatgaactc ttcatttact tcgggcaact
     2281 gtttttcctg aaccgctttg acggtgacag caaactgaac cgcctggcct ttcaattctt
     2341 ccgagtgata gtccgccggg aaagtcagcg ccagaacgcg ctcttcgccc ttggtggctc
     2401 ccagcaggcc atcttcaaaa cctggaatca tggaaccgga gcccagtaca agcttggagt
     2461 tttcagcgct gccgccctgg aaggcttctc catccttggt gccaacgaaa tcgatgatga
     2521 gctgatcgcc gttctgcgcc ggacgttcaa cgtcagcaaa ggtggcacgc tgctcacgca
     2581 gacgctggat catggtgtca atatcggcct ctgtaatgtc ggcaacagga cgggacacgc
     2641 tgatgctgga caagccctgc accgagacct gcggatagac ttcaaaggtg gcgacgaatt
     2701 caaaatcctc gcccggctga tccttggtgc gctcgatgct gggataacct gcaggattga
     2761 gtgcctgctg gttcatggct tcatagaagg agttgctgac gatttcacca atcacttctt
     2821 cgcgaatggc tttgccatag cggcgacgca cttcacgcac gggaaccttg ccaggacgga
     2881 agccgtcgat gcgaacgcgc ttggcggttt cgacgatttt ggtttcaacg gcgttatcaa
     2941 tcttgtcggc aggaacgccg acggtcatgc ggcggccgag tccggaggtg gtttccacgg
     3001 aaacttgcat atcaacctcg aatgctgttt gtagttgcgt aaatgatgcg tgagttgtaa
     3061 tcaatgagcc gattccgcaa aacagaaacc ggtaatatcc tttggacatt accggttttg
     3121 tagtctggcc tggcttgtcg gctatccaac tatctactat ccaaagaaag tggggtggac
     3181 gatggggctc gaacccacga caaccggaat cacaatccgg g
//
LOCUS       DASUEG010000916         5707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2001358, whole genome shotgun sequence.
ACCESSION   DASUEG010000916 DASUEG010000000
VERSION     DASUEG010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001358"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3188
                     /locus_tag="VFX11_17320"
     CDS             <1..3188
                     /locus_tag="VFX11_17320"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX4940435.1"
                     /translation="SPLQIQLVSSLYETGGRPVVRSYRARVWPGNIQFGVRPSFATGK
                     NPPSNSVVDFDLVQVDAEGALQAASSVQVNLVREDRQYYWEYNDNEGWHYQFTEKEYV
                     ERSQTMALQAQTGANKISFNVDWGRYRLEVKDPSSGAVTTVRFHAGEDWYADWQESQR
                     ADKSVRPDIVTLALDKGAYKPGDVAKLQVNSPHAGEALIMVESNQPLWLKRVRMDQPQ
                     TTVEIPIAAEWNRHDIHVSAVVLRPASRKEQITPNRAFGLVHLPLDRSDRKLSLELKA
                     PEKMQPDTDLRSEITVLDATGAPFANGYVTLAAVDVGVLNISEFKTPDPVSGFFGRRR
                     YSVDSRDIYGNLIELNDFAKAKIRFGGDADLARGGKEPDSDVQIVSLFNAPVKLDDKG
                     KAQVDLPIPYFNGKVRVMALAFGESKFASAEQETTIAAPLVTDLGLPRFLALGDTATA
                     AFDLHNMTGVAQNLKVEISTGAGLEMSTVEKTVALEADRKTTLPFTIKGDNLQQGSVK
                     IRVAGEGFTAIEREWKIGLRPAYPAEIRMANAILDPGAEFAVPSTLFKDLHGATAEML
                     LSVGNRANLNLPVHIRDLLHYPYGCLEQTSSSAYPYALATEQNQARFNMTPVTAQQRA
                     ERIDVALTRIAGMQASNGGFGLWDDTSPEEHWLTVYVTDFMLNVQEQGFSVPPAMLEK
                     ALNRLTEYVNRTGSLFEQRYTDDMDHYRFSYKAYAGYVLARLNKAPLASLRTLHDQFR
                     SQSESGLPLLHLGIALQLAGDQSRADAAINEALVKVRSEKNYLADYGSPVRDQAMMIY
                     LLLKHQIHTEHALTLSYSLADDLKQAQWLSTQERNALFMAGVMLESTTGENWMAKLRV
                     GDADELLTQSGNAYRKFEGDTLQKSIQIANTGNGRLFASAQVSGYPLTKPTPVEDQGY
                     RIRRQYFSPEGKAMQLSQVRGGDLVLVHLEVDSDTIAPESLIVDMLPAGFELENQNLE
                     HAVKLTDFRIDGKTLQELQGYAKVMHQEYRDDRYVAALDVRWDRGHLFYLMRAVTPGD
                     YRVPPPYVEDMYRAQRRGVGMTTDSVKVLPR"
     gene            3228..5657
                     /gene="pbpC"
                     /locus_tag="VFX11_17325"
     CDS             3228..5657
                     /gene="pbpC"
                     /locus_tag="VFX11_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX4940436.1"
                     /translation="MQTNPQDNATTPTQRPNFSTAAAGRFVRHVVPALFGNRWRQVAS
                     CCALLLALLFILDKTFPLNLPGDDALFARVVTDSSGRPLRVFPDRKGVWRYSIRLDEV
                     SPLYVEALLNYEDRAFWQHPGINPLAMLRALLQATLNGHVVSGGSTISMQVARLLHPH
                     ACTVLGKGYQMLRALQLEWHLSKREILQLYLNIAPFGGTVEGVQAAAYAYLDKPASAL
                     THGEAALLAVLPQSPTRIRPDRNPERAQVARDKVLDRMEKLGVWDARTVADAKQETVY
                     ASSLRPPQNAPLLASTLVQRHRESSVIRSTIDGELQRNLEDYVARYRNVLPAQSSLAI
                     LVVDNSDMRVRAYVGTADFGNQDRFGYLDMVQAVRSPGSTLKPFLFAMALEQGLIHSE
                     SLLSDAPRSGSSYRPGNFSGGFSGPVSAADALQRSLNIPAVDLLEQLGPQQFAARLQQ
                     GGLKLTIPGDQVPGLAVILGGAGATLEQLVTVYSSLARGGQSAPLRYRDDDPLLERYL
                     LDPGSAWIVWKILQSAPRPDRLHTLHQTLGQQPIAWKTGTSYGFRDAWSIGVSTRYSV
                     GVWVGRPDGTPMPGHFGAETAAPLMFDIFQQIARDDTGEPPQPASVSQRVICWPLGTD
                     ASRNAPGQCEQQRTAWVLHDTVPPTPNSGRNPLPLWVDAGTGMRVTPLCDGVQAQATQ
                     IVLWPAAIEPWLPGSARRAQRIPPLDARCHEDQPLQLPAPRILGLEDNSRLTAVQADA
                     MPQVDLSVAGGVGELQWYINGEYRFSSKANQTVRHRFATAGRYEIVVVDEQGQTDRRR
                     VKVERADLAYY"
BASE COUNT         1102 a         1674 c         1870 g         1061 t
ORIGIN      
        1 actcgccgct gcagatccag ctggtgagca gcctgtatga aaccggcggt cgcccggtgg
       61 tgcgcagtta ccgcgcccgc gtctggcccg gcaatatcca gttcggcgtg cggccatcgt
      121 tcgccaccgg caagaatccg ccatccaatt ccgtggtgga tttcgatctg gtgcaggtgg
      181 atgcggaagg cgcattacag gcggccagca gtgtgcaggt gaatctggtg cgcgaggatc
      241 gccagtatta ctgggaatat aacgacaacg aaggctggca ttaccagttc accgaaaaag
      301 aatatgtcga gcgttcgcag acaatggcgc tgcaggcgca gaccggcgcc aataaaattt
      361 ccttcaacgt ggactggggc cgctatcgcc tggaagtaaa agatccgtcc agcggcgccg
      421 tgaccaccgt gcgttttcat gccggtgaag actggtacgc cgactggcag gaaagccagc
      481 gcgctgacaa atccgtgcgc cccgatatcg tgacgctggc gctggacaag ggcgcctaca
      541 aacccggtga cgtggcgaaa ctgcaggtga acagcccgca cgcgggtgaa gcgctgatta
      601 tggtggagtc gaaccagccc ctgtggctca agcgcgtgcg catggaccag ccgcagacca
      661 ccgtggaaat cccgattgcc gccgagtgga accggcacga catccatgtg tccgccgtgg
      721 tgctgcgccc cgccagccgc aaggaacaga tcacgcccaa tcgcgcgttt ggtctggtgc
      781 atctgccgct ggatcgcagc gaccgcaagt tgagcctgga actgaaagcg ccggaaaaaa
      841 tgcagccgga taccgacctg cgtagcgaga tcaccgtgct ggatgccacc ggcgcgccct
      901 tcgccaacgg ctatgtcacg ctggcggcag tggatgtggg tgtgctcaat atcagcgaat
      961 tcaaaacccc cgatccggtg tctggctttt tcggtcgccg ccgttacagc gtggacagcc
     1021 gcgacattta cggcaacctg atcgagctga atgattttgc caaggcgaag atccgcttcg
     1081 gtggtgacgc tgatctggca cgcggtggca aggaaccgga ttccgatgtg cagatcgtgt
     1141 cgctgttcaa cgcgccggtg aaactggatg acaagggcaa ggcgcaggtc gatctgccga
     1201 ttccctattt caacggcaag gtgcgggtga tggcgctggc gttcggtgaa tcgaaattcg
     1261 ccagtgccga acaggaaacc accatcgccg caccgctggt gacggatctg ggtctgccgc
     1321 gtttcctggc gctgggcgat accgccactg ccgcgttcga tctgcacaac atgaccggcg
     1381 tggcgcagaa cctgaaagtg gaaatcagca ctggcgcggg gctggaaatg tcgacggtgg
     1441 aaaagactgt agcactggag gccgatcgca aaaccacact gccgttcacc atcaagggtg
     1501 acaacctgca gcagggcagc gtgaaaatcc gcgtggcggg agaaggtttc accgccatcg
     1561 aacgggaatg gaagatcggt ttgcgtccgg cctatccagc ggaaatccgc atggccaatg
     1621 cgatcctgga cccgggtgcg gaatttgccg tgccatcaac gctgttcaag gatctgcatg
     1681 gcgccacggc ggaaatgttg ctgtcggtgg gcaacagggc caacctgaat ctgcccgtgc
     1741 acatccgcga tctgctgcat tatccctacg gatgtctgga gcagaccagc agcagcgcct
     1801 atccgtatgc gctggccacc gagcagaatc aggcccgctt caacatgacg ccggtgaccg
     1861 cgcagcagcg cgccgaacgc atcgatgtgg cactgacgcg cattgccggc atgcaggcca
     1921 gcaacggtgg tttcggtttg tgggacgaca cgtcgccgga agaacactgg ctcaccgtgt
     1981 atgtcaccga cttcatgctc aacgtgcagg agcagggctt ttccgtgccg ccggcgatgc
     2041 tggaaaaagc gctgaaccgg ctcaccgaat atgtgaaccg cactggcagc ctgttcgagc
     2101 agcgctacac cgacgacatg gatcactacc gcttctccta caaggcctat gccggttacg
     2161 tgctggcgcg gctgaacaag gcgccgctgg cctcgctgcg caccctgcac gaccagttcc
     2221 gcagccagag cgaatccggt ttgccgctgc tgcatctggg cattgcgctg cagctggcgg
     2281 gggatcagtc ccgcgccgat gccgccatca acgaggcgct ggtgaaggtg cgcagtgaaa
     2341 agaattatct ggcggattac ggcagcccgg tgcgcgacca ggccatgatg atttacctgc
     2401 tgctgaagca ccagattcac accgagcatg cgctgaccct gagttacagc ctggccgatg
     2461 atcttaagca ggcgcagtgg ctgagcacgc aggaacgcaa cgcgctgttc atggccggcg
     2521 tgatgctgga atccaccacc ggcgaaaact ggatggcgaa attgcgggtg ggcgatgcgg
     2581 atgaactgct gacgcaatcc ggcaatgcct atcgcaagtt cgagggcgac accttgcaga
     2641 aatccattca gattgccaac accggtaacg gtcgcctgtt tgcgtcggca caggtgtcgg
     2701 gctatccgct caccaaaccc acgccggtgg aagaccaggg ctaccgcatc cgccgtcagt
     2761 atttctcgcc ggaaggcaag gcgatgcagc tgagtcaggt gcgcggcggc gatctggtgc
     2821 tggtgcatct ggaagtggat tccgacacca tcgcgccgga atccctgatc gtggacatgc
     2881 tgcccgccgg ctttgaactg gaaaaccaga acctggaaca tgcagtgaag ctgacggatt
     2941 ttcgcatcga cggcaaaacc ctgcaggaat tgcagggcta cgccaaggtg atgcaccagg
     3001 aataccgcga tgaccgttac gtggcagcgc tggatgtgcg ctgggatcgc ggccacctgt
     3061 tctacctgat gcgtgcggtg acaccgggcg actaccgcgt gccgccaccc tatgtggagg
     3121 acatgtaccg ggcgcagcgc cgtggcgtgg gcatgaccac tgacagcgtg aaagtgttgc
     3181 cgcgttaagc agtaaagcgc aattgcagtg gcaggatggg attgaacgtg cagacgaacc
     3241 cgcaggacaa cgcaacaacg ccgacgcagc ggccgaattt ttcaacggcc gctgcaggca
     3301 ggtttgtgcg gcatgtggtg cccgcgttgt ttggcaatcg ctggcgtcag gtcgcatcct
     3361 gctgtgcatt gttgctggcg ctgctgttca tcctcgataa aacctttccg ttgaatctgc
     3421 ccggtgatga tgccctgttc gcccgcgttg tcaccgacag cagcgggcgt ccgctgcggg
     3481 tgtttcccga ccgcaagggg gtgtggcgct attccatcag gctcgatgaa gtgtcgccgc
     3541 tgtatgtgga agcgctgctg aattacgaag accgcgcgtt ctggcaacac cccggcatca
     3601 atccgctggc gatgctgcgg gccttgttgc aggccacgct gaacggtcat gtggtatccg
     3661 gcggctccac catcagcatg caggtggcgc ggctgctgca cccgcatgcg tgcacggtgt
     3721 tgggcaaggg ctaccagatg ctgcgggcac tgcagttgga atggcatttg agcaagcggg
     3781 aaatattgca gctgtatctg aatatcgcgc cgtttggtgg cacggtggag ggcgtgcagg
     3841 cggcggccta tgcctatctc gacaagccgg ccagtgcgct cacccacggt gaagcggcgc
     3901 tgcttgctgt actgccacaa tcgcccacgc ggattcgccc ggaccgcaat cccgaacggg
     3961 cgcaggtggc acgggacaag gtgctcgacc gcatggaaaa actcggcgtg tgggatgcgc
     4021 gcacggtggc ggatgcgaaa caggaaactg tgtatgccag cagcctgcgt ccgccgcaga
     4081 atgcgccgct gctggccagc acgcttgttc agcgtcatcg cgaatccagc gtgattcgca
     4141 gcaccatcga cggcgagctg cagcgcaatc tggaagatta tgtggcgcgc tatcgcaacg
     4201 tgttgccggc gcaaagttcg ctggcgatcc tggtggtgga caacagtgac atgcgcgtgc
     4261 gcgcctacgt gggcacggcg gattttggta atcaggaccg ctttggttat ctcgacatgg
     4321 tgcaggcggt gcgctcgccc ggctccacgc tgaaaccgtt tctgtttgcg atggcgctgg
     4381 agcagggcct gatccattcc gaatccctgc tgtccgatgc gccgcgcagt ggcagcagtt
     4441 atcggccggg gaatttttcc ggtggcttca gtggcccggt gtcggcggcg gatgccttgc
     4501 aacgctcgct gaatattcct gccgtggatc tgctggaaca gctgggtccg caacaatttg
     4561 ctgcgcgtct gcagcagggt ggactgaagc tgaccattcc cggcgatcag gtgccagggc
     4621 tggcggtgat tctgggcggt gccggtgcca cgctggagca gctggtgacc gtgtacagtt
     4681 cgctggcgcg tggcgggcag agcgcgccac tgcgctatcg cgatgatgat ccgctgctgg
     4741 aacggtacct gctggatccg ggcagtgcct ggatcgtatg gaaaatcctg cagtcggcgc
     4801 cgcgcccgga tcgtctgcac acgctgcacc agacgctggg tcagcaaccc atcgcctgga
     4861 agaccggtac cagttacggt ttccgcgatg cctggtccat tggtgtcagc acccgctaca
     4921 gcgtgggtgt gtgggtgggg cggccggatg gtacgcccat gcccggccat ttcggtgcag
     4981 aaactgccgc gccgctgatg ttcgatattt tccagcagat tgcccgcgac gataccggcg
     5041 aaccaccgca accggccagc gtaagccagc gcgtcatctg ctggccgctg ggcacggatg
     5101 cgtcgcgcaa tgcgcccggt cagtgtgaac agcagcgcac ggcctgggtg ctgcacgaca
     5161 cggtgccgcc cacgccgaac agcggccgca atccgttgcc tctgtgggtg gatgcgggca
     5221 ctggcatgcg cgtgacgcca ctgtgtgacg gcgtgcaggc gcaggcgacg caaatcgtgc
     5281 tctggcctgc cgcgatcgag ccctggttgc ccggttccgc ccgccgcgcc cagcgcattc
     5341 cgccattgga tgcccgttgc cacgaggacc agccgctgca gttgcccgcg ccgcggattc
     5401 tggggctgga ggataacagc cgcctcacgg ccgtgcaggc cgatgccatg ccgcaggtgg
     5461 atttgagtgt ggcgggtggc gtgggggaat tgcagtggta catcaacggc gaataccgct
     5521 tcagcagcaa ggctaaccag acagtgcgtc atcgttttgc cacggccggc cgctatgaaa
     5581 tcgtggtggt ggatgagcaa gggcagacgg accggcggcg ggtgaaggtg gagcgggcgg
     5641 atctggccta ctattaaaga gttggtttca tgcgatttac ttaggccgtg gcgccggggg
     5701 ctggatg
//
LOCUS       DASUEG010000917         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1338417, whole genome shotgun sequence.
ACCESSION   DASUEG010000917 DASUEG010000000
VERSION     DASUEG010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338417"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFX11_17330"
     CDS             <1..836
                     /locus_tag="VFX11_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX4940437.1"
                     /translation="TTGNSYSASGALWAAHDAMKKLGLVQIGPSGKMAGKAMVVGATG
                     AIGSVCARLLAKAVDDLYLVSPEAGKLLALKEDIEHETPNARVHVMAHADKCLGEMDM
                     IVTATSGAGKRILDILQVKPGCVITDVARPLDIPAEDVAKRPDVLVIESGEIRLPGEV
                     HMKNIGLPKNVAYACLAETIVLALEGRFENYTLGRNIEWEKVREIYKLGLKHGMQLAS
                     ISGVNGVYSDADLAQVRQLALEKLGQPLVPARETLPELAPATTVEFSEIIKRNRKTVD
                     S"
     gene            complement(872..>2030)
                     /locus_tag="VFX11_17335"
     CDS             complement(872..>2030)
                     /locus_tag="VFX11_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX4940438.1"
                     /translation="VAGREEARLIYLGVAHTLADDGGKRLVFDIGGGSTEFIIGKRFE
                     PLLTESLHMGCVAYRDRFFANGKISEQGFKKAVMTARTELAAIEATYRKKGWDNVVGA
                     SGSVKAIEGALQENGWFKDAIDLAGLHKLRDRLLTFNSLDAIDIPGIKADRRHILPSG
                     LAILIAIFEQLGIKSAHYSDGAMREGILYDLLGRHVHEDVRERSVLAMMNRYSVDTAQ
                     AERVLQSALKAFGQVKKEWDLGEYCREWLRWSALTHEIGMAVSHSGFHKHGAYLLTHS
                     DLPGFTRQDQKVMAVLVGNHRRKLKESDWQELPSHLQEKVLRLVLLLRLSALFHRARR
                     EDLVPDFRLAVDGKGARLRFSAKWLEEHPLLQAELQEEQDVWKKIGMKLEF"
BASE COUNT          419 a          669 c          578 g          364 t
ORIGIN      
        1 tcaccaccgg caacagctac agcgcgtccg gcgccttgtg ggcggcccat gacgcgatga
       61 aaaaactcgg cctggtccag atcggcccca gcggcaagat ggcgggcaag gcaatggtgg
      121 tgggcgcaac cggcgccatc ggttccgtct gcgcacgact gctggcaaaa gccgtggacg
      181 atctctatct ggtgtcaccg gaagccggca agctgctggc actgaaggaa gacatcgagc
      241 acgaaactcc caatgcccgc gtccatgtga tggcgcacgc ggacaaatgc ctgggcgaga
      301 tggacatgat cgtcaccgcc acctccggcg ccggcaaacg catcctcgac atcctgcagg
      361 taaaacccgg ctgcgtgatc accgatgtgg cgcgtccgct cgacattccc gccgaggacg
      421 tcgccaagcg ccccgacgtg ctggtgatcg aatccggcga gatccgcctg cccggtgagg
      481 tgcacatgaa aaacatcgga ctgccgaaaa acgtcgccta tgcctgtctg gcggaaacca
      541 tcgtgctggc gctggaaggc cgcttcgaga attacaccct gggccgcaat atcgagtggg
      601 aaaaggtgcg ggagatttac aagctgggac tgaaacacgg catgcagctg gcgtcgattt
      661 ccggcgtgaa tggcgtgtac agcgacgcgg atctggcgca ggtgcggcaa ctggcgctgg
      721 agaaactggg ccagccactg gtaccggctc gcgaaacctt gccggaactg gcgccagcca
      781 ccacggtgga attcagcgaa atcatcaagc gcaacaggaa aaccgtggat tcctgatcaa
      841 tcccgccggc gctgggggcg caactgcgcc ctcagaactc cagtttcatg ccgattttct
      901 tccacacatc ctgctcttcc tgcagttctg cctgcagcag cggatgctcc tccagccatt
      961 tggccgaaaa ccgcaggcgc gcgcccttgc catccacggc caggcgaaaa tccggcacca
     1021 gatcctcgcg ccgggcccga tgaaacagcg cgctcagtcg cagcagcaat accagtcgca
     1081 acactttctc ctgcaggtgc gatggcaatt cctgccagtc gctttccttc agcttgcgcc
     1141 ggtgattgcc caccagcacc gccatcactt tctggtcctg gcgggtgaag cccggaaggt
     1201 ctgaatgcgt gagcagatag gcaccgtgct tgtgaaaacc cgagtgggac actgccatgc
     1261 cgatttcatg ggtcagggct gaccagcgca gccattcgcg gcaatactcg cccagatccc
     1321 attccttctt cacctggccg aacgccttca gcgccgactg cagtacccgt tcggcctggg
     1381 cagtatccac gctgtaccgg ttcatcatcg ccagcaccga acgttcgcgc acatcctcat
     1441 gcacatgccg ccccagcagg tcgtacagaa tgccttcgcg catggcaccg tcggaataat
     1501 gcgccgactt gatgcccagc tgctcgaaga tcgcgatcag gatggcaagg ccactgggca
     1561 ggatatggcg gcggtccgcc ttgataccgg gaatgtcgat ggcgtccagg gaattgaacg
     1621 tcagcagccg gtcccgcaat ttgtgcaggc ccgccagatc aatggcgtcc ttgaaccagc
     1681 cattttcctg cagcgcgccc tcaatggcct tcaccgagcc cgaggcgccc accacgttat
     1741 cccaaccttt cttgcggtac gtcgcttcta tcgccgccag ctcggtgcgt gccgtcatta
     1801 cggccttttt gaaaccctgc tcgctgatct tgccgttggc aaaaaaacgg tcgcgatagg
     1861 caacgcagcc catgtgcagg gactcggtca acagcggctc aaaccgtttg ccaataatga
     1921 attccgtaga accgccacca atgtcgaaca ccagccgctt gccgccatca tccgccagcg
     1981 tgtgcgccac ccccaggtag atcaggcgcg cctcttcacg gccggccacc
//
LOCUS       DASUEG010000918         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_654168, whole genome shotgun sequence.
ACCESSION   DASUEG010000918 DASUEG010000000
VERSION     DASUEG010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654168"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFX11_17340"
     CDS             complement(<1..714)
                     /locus_tag="VFX11_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4940439.1"
                     /translation="MTDFETADFFTDTSLIENPFPYFEYLRSQGPIARLPHRNVLAVT
                     GYAEAIEILNDPQTFSSANAATGPIPDLPFEPEGDDITEQLNAHRDEMIATGLVVAKD
                     GKEHANHRSLMMNLFTPTRLKANEEYMWNLTSTLLDKFVDQGHCDLIASYATPFATLV
                     IADLLGIPENDRITFCEKLGPPPGAVGGEVDYMDSQVLGMSFLFEYFGQYLAERRETP
                     KGDILSQFAQAKFPDGSTPS"
     gene            complement(1002..1766)
                     /locus_tag="VFX11_17345"
     CDS             complement(1002..1766)
                     /locus_tag="VFX11_17345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4940440.1"
                     /translation="MLAWNWDNTGLPTLMLVHGFGAHAHWWSYLAPYFRDRYRIVALD
                     LPGMGDSEPPAVYDDDCFARAITGCIEEHQLQPVCIIGHSFGGAQSLRAIAMSPHLFN
                     RGIVVDTNVRLPPEPLIRRLQPKGSHKRSPTRADCMARFRLVPPQPNYIDALVHFIAH
                     HSCTGEPQNWHWKSDPNCLNAGEIENSDILRAVSTPIDMIYGEKSFLNIENKPARVLS
                     HFPRAGTLLTIPNAGHHIMVDHPLELIAAIKTLLLN"
     gene            complement(1993..3678)
                     /locus_tag="VFX11_17350"
     CDS             complement(1993..3678)
                     /locus_tag="VFX11_17350"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4940441.1"
                     /translation="MLFLAFSASSTQASFTDNLTIGNPKALSLGHAVTADPPGIDSVH
                     FNPAGLTGLKGRQFHLKGVYGIFEMEMEMGEYGEYQQNLIDNWVNDPNHSSYFVDDEG
                     NLTAAGQAFIYDEAHNAKSEVEGPTIMLPGTGMVDLPFAGGVMGGASFTPTGSRFTFA
                     TNVYAPMMNGLHRAKDDPGRYAQERVAFTVITYFSPSVAYELTDELSVGLAITFNYAG
                     MGMELPAREPHAGIFFLGSSWTQDNFCNGKPAGNDPTDPDTNICTTVVNAYEEYANLS
                     FEVEQLDALGYNLGLLWSPQPWLSLGVAYNSKINADMDGNFEFPVNDNFKTFLVGLMS
                     GSTYSNAERGLGSALDLPSVDEIQNDAKGKMNVQYELPQHLAAGISVQILPRWKTNFD
                     VKWTEWSAFSNINLNFGKSVGLLKLGGVVDTLLYNGDNGITGNSVKYKLGLQDVTYWG
                     MGTEYQYNDQLALRAGFESRPSAVPDDHPNAFIPINDGKLYSAGFAYELKDDNHIDFA
                     LGYFNSKTHFPPCSADVGNGCNLNNVVYPVFQGQDLKTNVTFYLIELMYSRHF"
     gene            4253..5017
                     /locus_tag="VFX11_17355"
     CDS             4253..5017
                     /locus_tag="VFX11_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940442.1"
                     /translation="MKLIRQGEAKKLMHTKDQRICLWLVPVFGSLFIFAFWNFKGFFP
                     PMSPTMSAEAVAAFYRDNLGHIRSSMILCNVIGVSLVPFYTVIVVQMMRMLNASRVFA
                     YSYLIAVVNGGTLFALADLAWLNAAFRPDRDPQLTMLLNDLAWFAFITPVGFVIVQNF
                     CLAMAVYLDKGLRPVFPRWVAHFNIVTAVLIAPSAFALMYKTGPLAWDGAISFWLHIS
                     TYGVYVAVMFFVVRAAIRQQDEEALAAQSSWQERLA"
     gene            5014..5790
                     /locus_tag="VFX11_17360"
     CDS             5014..5790
                     /locus_tag="VFX11_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940443.1"
                     /translation="MTGALMTPVTGSNPRTGRFAENPKLDQWICFWSIPVFYVLFGII
                     FVMLGRIMPPPTPTMSTADIVAFMTSPNLSLAVVLLALSLGMYALNSGLMLTQMKRME
                     GVSPALRYAYIAVLGVGGLPGCLFPGYMFALGAFRPEYDPDILAMLYDLGFLCFVGSL
                     GCFVIQYVVFTIAVFLDRRGIFPRWLGYFSIWTLVTELVATPVFVTQTGPYAWDGLLA
                     FYQGTVIWVTWQTCVTICLYKAIKSQPLEELGKPFRPNEM"
     gene            5812..>6120
                     /locus_tag="VFX11_17365"
     CDS             5812..>6120
                     /locus_tag="VFX11_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX4940444.1"
                     /translation="MLNGNGVAMGQDLDTGVATGRAIRSRGRLPGQPAMWFFVIGDLW
                     IFTCYFACYVFDRGQNPEAFLRGQQTLSQGVGAFNTMLLLTSSLWVAFCVQAVRLGAI
                     A"
BASE COUNT         1331 a         1486 c         1703 g         1600 t
ORIGIN      
        1 cgacggcgtt gatccgtccg gaaacttggc ctgggcaaac tggctgagaa tatcgccttt
       61 gggtgtttcg cggcgctcgg caaggtactg cccaaaatat tcaaacagga agctcatgcc
      121 gagaacctgg gaatccatgt aatccacttc accacccact gcaccgggcg gcgggcccag
      181 cttttcgcag aaggtgatgc ggtcgttttc cggaataccc agcaggtcgg caataaccag
      241 tgttgcaaac ggagtcgcat aggaagcgat cagatcgcag tggccctggt ccacaaattt
      301 gtcgagcaac gtgctggtga ggttccacat gtactcttcg ttggctttca aacgggtggg
      361 cgtgaacagg ttcatcatca gagagcgatg attggcatgt tctttaccgt ctttggcaac
      421 caccagtccg gtcgcaatca tttcgtcgcg atgcgcattc aattgctcgg tgatgtcgtc
      481 gccttcaggt tcaaaaggca aatcggggat aggccctgtt gcggcattgg ccgacgaaaa
      541 agtctgggga tcgttgagaa tctcaattgc ttctgcatag ccggtaacgg ccagaacatt
      601 ccggtggggc agacgggcaa tgggcccctg gctgcgcagg tactcgaaat aggggaacgg
      661 gttttcaatc agcgaagtgt cagtaaaaaa atcggcggtt tcaaaatcag tcatgtttcc
      721 ctctcatttc tttttaaaag gctttcaggg ctttgggcca ggtgactggc cgtcgggttt
      781 cagcgatttt ggctggaaag tcggataagt gcctgatttc aggagcgctg tgcctgaatc
      841 aagatgtgaa actatgtggt tcatattaat cgggcagtat aaaaagtcaa tagtgggatt
      901 ccggatagtg cgggcaggcc ggtggcgggt tttgaatggc ggacttgccg ccattccagc
      961 ttttttcacc agaaggacag gggcaacttt gaagacgggg ttcaattcag caacaaggtt
     1021 ttgatggcgg cgataagctc cagtggatga tccaccatga tgtggtgtcc agcgttgggg
     1081 attgtgagca atgttcccgc gcggggaaaa tggctcagaa cccgcgccgg tttgttttca
     1141 atattgagga aactcttttc accgtaaatc atgtcgatcg gggtgctgac cgcacgcaat
     1201 atatcggaat tttcaatttc gcctgcgttg aggcagttgg gatcggattt ccagtgccaa
     1261 ttctgcggct caccggtaca ggaatggtgc gcgatgaagt gcaccagcgc gtcgatatag
     1321 ttcggctgcg gtggcaccag gcgaaatcgt gccatgcaat cagcccgtgt cgggctgcgt
     1381 ttatggctgc ctttgggctg caatcggcgg atgagcggct ccggcggcag gcgcacattg
     1441 gtgtccacca caatgccacg gttgaacagg tggggtgaca tcgctattgc ccgcaatgat
     1501 tgtgctccgc cgaaactgtg gccgatgatg caaaccggtt gcagctgatg ttcctcaatg
     1561 caacctgtga tggcgcgggc aaagcagtca tcgtcataaa ctgcgggtgg ttcgctgtcg
     1621 cccattccgg gcagatccag tgccactatc cgataacgat cccgaaagta tggagccaga
     1681 tagctccacc aatgcgcgtg agcgccgaat ccatgtacca gcatcagcgt gggcaatccg
     1741 gtgttgtccc agttccacgc cagcatgtgc actgatctgc cggcaatccg cgtctgtacc
     1801 gataggttcg gtatgccgat attgtgcaga aaccaggggg gagcgttgtc caatggttga
     1861 taagagtcgt gtctggttga accaacctcg ctcatgctgc gaacctcaat acagtgcggc
     1921 cattggcaaa aacgcatcca atctgcgaac tgccagtgaa ttgtgcgtag aaaaggaatg
     1981 gatagtaaaa cgctaaaaat gtcgactgta catcagctca atcagataga aagttacatt
     2041 ggttttcaga tcctgaccct gaaataccgg atagaccacg ttattcagat tgcagccatt
     2101 tccaacatcg gcagaacagg gtggaaagtg ggtttttgaa ttgaagtatc cgagcgcaaa
     2161 atcaatatgg ttatcatctt tcagttcgta ggcaaatcca gctgaataca gtttgccatc
     2221 gttgatgggg atgaacgcgt tgggatgatc atccggaacg gcagaagggc gcgattcaaa
     2281 gccagcccgc agtgccagtt gatcgttgta ctggtattct gtgcccattc cccaataggt
     2341 tacatcctgt aatcccagct tgtatttgac agaattgccg gtaatgccgt tgtcgccatt
     2401 gtacagaagt gtgtcaacca cgccgcccag cttgagcaag ccgacagatt tgccaaaatt
     2461 caagttaata ttgctgaaag cagaccattc cgtccatttg acgtcgaaat tggttttcca
     2521 gcgcgggagg atctggacgc ttattccggc cgccagatgt tgtggcagtt catactggac
     2581 gttcatcttg cctttggcat cattctggat ctcgtcaacc gatggcagat cgagtgccga
     2641 tccaaggccc cgttcggcgt tggagtaggt ggacccgctc atcagtccaa ccagaaatgt
     2701 tttgaaattg tcattgaccg gaaattcgaa attaccgtcc atatcagcgt tgatcttgga
     2761 gttgtacgcg acacccagtg acagccaggg ttgggggctc cacaaaagtc cgaggttgta
     2821 tcccagcgca tccagctgtt ccacctcaaa cgacaagttg gcgtattcct cgtatgcgtt
     2881 tacaacggtg gtacatatat tggtgtcggg atctgtcggg tcgttacctg ccggtttccc
     2941 gttacagaaa ttgtcctggg tccaggacga acccagaaaa aatatgcccg catggggttc
     3001 tctggccgga agctccatcc ccatgccagc ataattgaag gtaattgcca gaccgactga
     3061 caactcatcc gtgagttcgt acgctactga aggcgaaaaa taggtgatga cggtaaacgc
     3121 gacgcgttcc tgggcgtacc ggccggggtc atctttcgcc cgatggagcc cattcatcat
     3181 gggggcatag acgttcgtgg caaacgtaaa gcggctaccg gtgggcgtga aggacgcgcc
     3241 tcccatcacg cctccagcaa aaggcaaatc caccatgccg gttccaggca gcatgatggt
     3301 tggcccctcc acctcgctct tggcgttgtg ggcttcgtcg taaataaagg cttgccccgc
     3361 agcggtcaga ttcccttcgt catcgacgaa gtaggaagag tgattgggat cgtttaccca
     3421 gttgtcaatc agattctgct gatactctcc gtactccccc atctccattt ccatttcgaa
     3481 tattccatag actcccttca aatgaaattg acgccctttc agcccggtaa gaccggcagg
     3541 attgaagtgg acggagtcga tccctggcgg gtcggcggta acggcatgcc ccagcgacaa
     3601 cgcttttgga ttgccaatcg tcaaattgtc tgtgaaggaa gcctgtgtcg aggaagcgga
     3661 gaacgccaga aacaacaaaa aacacagata gctgctttga ttcgtcttgt accggcaatt
     3721 gaacaaggtg cttctataaa tcatatcttc tgatttttta attgcttttc tgaatggtgc
     3781 ttgagatcta cgctatgaca gggcgtactt ttcaagcttg tatcgaaaat caatctatct
     3841 gatcggatat tgtggcgcaa cgccagtttg cctgtttcgg caatagtttt gttgtttaga
     3901 tgtaatgaat ttcaggttgt gcgtagctgt tgttttatcg gttgaatttg attcctgttc
     3961 tttcaatggt ttgaatggat cagtcgattt tcgacagatt gaacaagcgc cgctgggtgt
     4021 agcttgaatg gaagtatgag tgtcattttt tttgctaacg cagccatttt acctaacaag
     4081 gtttagtgga gccgtctgct gcggtttttc atcaaacatg aacccgctat gaagctctgt
     4141 ttcatctgtt gaatgaatga gtcagcatca cttcggttgt tgtaaaccgg gagtcgattg
     4201 aatactgtag tatcgttgcg aaacggaaag acttgatcgt gtacggttgc agttgaaatt
     4261 gattcgacag ggcgaggcca aaaaactcat gcatacaaaa gatcagcgca tttgcctgtg
     4321 gctggttccg gtttttgggt cattgttcat tttcgccttc tggaacttca agggattctt
     4381 tccgccaatg tcaccaacga tgagcgcaga agcagttgcc gcgttctacc gtgacaatct
     4441 tggccatatc cggtccagca tgattctttg caacgtgata ggtgtttcgc ttgttccttt
     4501 ttacaccgtc attgtcgtgc agatgatgcg gatgctgaac gccagtcggg tgtttgccta
     4561 ttcgtacctg atcgcagtgg tcaatggggg tactctgttc gcactggctg accttgcgtg
     4621 gctcaatgct gctttccgcc ctgatcgcga tcctcaactg acgatgctgc ttaacgacct
     4681 ggcctggttt gcttttatta caccggtggg tttcgtgatc gtacagaatt tctgtctggc
     4741 gatggcagtc tatctggaca aggggttgcg accggtgttt ccacgctggg ttgctcattt
     4801 caatatcgtc acggcggtgc tgatcgctcc aagtgctttt gcgttgatgt acaagacggg
     4861 gcctttggca tgggatggtg caatcagctt ctggctgcac atttcgactt atggagtgta
     4921 cgtggcagtt atgtttttcg tggtgcgtgc ggctatccgc caacaggatg aagaagcctt
     4981 ggctgcgcaa tcctcctggc aggagaggct ggcatgacag gtgccctcat gaccccggtc
     5041 acagggagta atccccgtac gggcagattt gctgaaaatc ccaaactgga tcagtggatc
     5101 tgcttctggt ccattccggt tttttatgtg ctgttcggca tcatattcgt catgctcggc
     5161 cgaatcatgc cgccacccac accgaccatg tcgaccgcag atatcgtcgc cttcatgaca
     5221 tcgcccaacc tgtcgctggc tgtcgttttg ctggcactgt cgctgggaat gtacgccttg
     5281 aattcgggcc tgatgctgac gcaaatgaag cgcatggagg gcgtcagccc tgcgctgcgt
     5341 tatgcctata tagcggtgtt gggcgtcggc ggcctgcccg gttgcctgtt tcccggctac
     5401 atgtttgcgc tgggcgcgtt tcgcccggag tatgatcctg acattctggc gatgctgtac
     5461 gatcttggct ttctttgctt tgtgggctct ctgggttgct tcgtgatcca gtatgtcgtt
     5521 ttcacgatcg cggtttttct agatcggcgc ggcattttcc cccgatggct cggctacttc
     5581 agcatatgga cgctggtaac ggagttggtg gcaacacctg tttttgtgac gcagaccggt
     5641 ccctatgcct gggatggtct gcttgccttt tatcaaggca ccgtgatttg ggttacctgg
     5701 caaacctgcg tcacgatctg tctgtacaag gcaataaaga gccagccatt ggaggagttg
     5761 gggaaaccct tcagacccaa tgaaatgtga ttgctccggg aagggtggac aatgctgaac
     5821 ggcaacgggg tggccatggg gcaagacctg gataccggcg tggcaacagg tcgggcaatc
     5881 cggtctcggg gacgtttacc gggacagccg gcgatgtggt tttttgtgat cggcgatttg
     5941 tggatattca cctgttactt tgcctgctat gtgtttgacc gcgggcaaaa tcccgaggca
     6001 tttctgcggg gccagcaaac gctcagtcag ggagtcgggg cattcaatac catgctgctg
     6061 ttaaccagtt cgctgtgggt tgcattctgc gttcaggcgg tacggttggg agccattgcg
//
LOCUS       DASUEG010000919         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_391202, whole genome shotgun sequence.
ACCESSION   DASUEG010000919 DASUEG010000000
VERSION     DASUEG010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391202"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFX11_17370"
     CDS             <1..728
                     /locus_tag="VFX11_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940445.1"
                     /translation="GIGRATAERFYNAGWCVGLFDISAQPLAELSRTWQPERFRIQVA
                     DVTQEAQIGAAIDRFVADVGKLDVLFNCAGVLEIGDFESIPLQRHHQIIDINNKGVLN
                     CCHHALRHLKNNPGARVISMSSASSLYGVPGFASYSASKFFVKGLTEALNVEWSRYGI
                     RVMDVEPPFVKTPMLAGKQAAIIERMGVNLSADDIAEAVFAAAGDTSIHHPVSTPYKV
                     LRVARKFLPDVATRTLMKLMSGY"
     gene            complement(816..1652)
                     /locus_tag="VFX11_17375"
     CDS             complement(816..1652)
                     /locus_tag="VFX11_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940446.1"
                     /translation="MSHLKNKTLFITGASRGIGKEIALKAARDGANIVIAAKTTEPHP
                     KLPGTIYSAAEEIEKAGGRALPLVVDVREEAQVAEAVEKAVATFGGIDILVNNASAIN
                     LTGTLQVDMKRFDLMHSINFRGTFLCSKLCVPHLKKSANPHVLNLAPPLSLNPRWFAP
                     HLAYSMAKFGMSFCVLGMAEEFRNDNIAFNALWPRTAIATAAVQNHLGGDHTVRLSRL
                     PQIMADAAYEIFKRSSRDCTGNFFIDDEVLTEAGVKDFRQYQVDTSLEPHQLIPDFFI
                     DV"
     gene            1925..2455
                     /locus_tag="VFX11_17380"
     CDS             1925..2455
                     /locus_tag="VFX11_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4940447.1"
                     /translation="MDQRASTRTSLPDFCQLQAGQIMSVPVQTVYEGWSVKRLMKFFV
                     EAEISGAPVIASDGELVGVVSISDIVRFENLPPKEKISVSNMDCYNEYVGYRLSESEL
                     KAMFDHADENCTVNQIMTPSVIAVSQETSLPEIAALMREQNIHRVFVQNQNRVVGVVS
                     TSNVLGVIASMCEVSR"
     gene            complement(2503..>2687)
                     /locus_tag="VFX11_17385"
     CDS             complement(2503..>2687)
                     /locus_tag="VFX11_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4940448.1"
                     /translation="TDREKQILKAIASGDSNKMIARKLDITEGTVKVHVKKVLKKLSF
                     RSRVEAAVWAVSQGIR"
BASE COUNT          509 a          701 c          856 g          621 t
ORIGIN      
        1 ctggcattgg tcgcgccacg gctgaacgct tttacaatgc cggctggtgt gtcggcctgt
       61 ttgatatcag cgcgcagccg ttggcggagc tgagccgtac ctggcagcca gagcgttttc
      121 gtatccaggt ggccgatgtc acgcaggaag cccagatcgg tgcggccatt gaccgtttcg
      181 tggcggatgt gggcaagctg gatgtgctgt tcaattgcgc gggcgtgctg gagatcggtg
      241 attttgaatc catcccgctg caacgtcatc accagattat cgacatcaac aacaagggcg
      301 tgctgaattg ctgccatcac gcgctgcggc atctgaagaa caatccggga gcgcgggtga
      361 tcagcatgtc gtcggcgtca tcgttgtacg gcgtgccggg gtttgccagc tattccgcgt
      421 ccaagttctt tgtgaaggga ttgaccgagg cgttgaatgt ggagtggtcc cgttacggga
      481 tccgggtgat ggacgtcgag ccgccgtttg tgaaaactcc gatgctggca ggcaagcagg
      541 ccgccatcat tgagcgcatg ggggtgaatc tgtcggcgga cgatattgca gaagccgtgt
      601 tcgcggcagc gggggacacc agcattcatc accccgtcag cacgccgtac aaagtcctgc
      661 gggtggcgcg caagttcctg ccggatgtgg ctacacggac cctgatgaag ctgatgtccg
      721 gctattgaga aaatgcacgg cgggctgcca gtctgcgcag cccgccgatt tgtttttact
      781 gttgcggttt gaccgcccca tcctgtgccg atccgtcaca cgtcgatgaa gaaatccgga
      841 atcagctggt ggggttccag cgaggtgtcc acctgatact gacggaaatc ctttacgccg
      901 gcttcggtca gcacttcgtc atcaatgaag aaattcccgg tgcagtcccg gctgctgcgc
      961 ttgaagattt cgtacgcggc gtcggccatg atctgcggca gacgtgacag ccggacagtg
     1021 tgatcgccac ccaggtgatt ctgcactgca gccgtggcaa tcgcggtacg gggccagagc
     1081 gcgttgaacg cgatgttgtc gttgcggaat tcttcggcca tgcccagtac gcagaagctc
     1141 atgccgaatt ttgccatgct gtaggccagg tgcggtgcga accagcgcgg attcagactg
     1201 agcggcggcg ccaggttcag cacgtgcgga ttggcggatt ttttcaggtg cggcacgcac
     1261 agtttcgagc acaggaaggt gccgcggaag ttgatcgagt gcatcaggtc gaaccgcttc
     1321 atgtccacct gcagggtgcc ggtcaggttg atggcactgg cattgttcac caggatgtcg
     1381 ataccgccaa aggtggcaac ggctttttcc accgcttcgg caacctgggc ttcttcccgt
     1441 acgtccacca ccagcggcag cgcgcggcca ccggcttttt cgatttcctc ggcggcgctg
     1501 tagatggtgc ccggcagttt gggatggggc tcggtggttt tggcggcgat gacgatgttg
     1561 gcaccgtcgc gggcggcctt cagggcgatt tccttgccaa ttccgcggct ggcaccggtg
     1621 atgaacaggg ttttattctt gagatggctc atggtgtgct ccctctggcg tgtggtgagg
     1681 tcactataaa caaggatttt cccctgtggc ggggaatgcc ggttgaggtg aatgatttgt
     1741 catttgcggg tgccagatcc agtgcctggg gcttatccct ttggggatag cgtcggatct
     1801 ttcctgcgta atatcaataa accggcgcgg ggttgctgct atacgttaac ccagtggttc
     1861 ctgtttacct gcgacgcagg acagtgaacc ggatgtggta gagatcccga cgggagggac
     1921 cagaatggat cagagagcga gtaccagaac cagtttgccg gatttctgtc agttgcaggc
     1981 gggtcagatc atgtcggtac cggtgcagac cgtctacgaa ggctggtcgg tcaagcggct
     2041 gatgaaattt tttgtggaag ccgaaatttc cggcgcaccg gtaattgcca gcgatggcga
     2101 actggtgggg gttgtgtcaa tttcggatat cgtgcggttc gagaacctgc cgcccaagga
     2161 aaagatctcg gtgagcaaca tggattgcta caacgagtat gtgggttaca ggctgtcgga
     2221 gtccgagctg aaagccatgt ttgatcacgc tgacgagaac tgcacggtga accagatcat
     2281 gacgccgtcg gtgatcgctg tcagtcagga gacgtcattg ccggaaattg ccgcgctgat
     2341 gcgtgagcag aatatccatc gggtattcgt gcagaaccag aaccgggttg tgggtgtggt
     2401 gagcacatcc aatgtgctgg gtgtcattgc gtccatgtgt gaggtaagcc gataagtcag
     2461 actgcacaag cgcggggcac gaggtgtgcc ccgcctcctg cctcagcgaa ttccctgact
     2521 cactgcccac acggctgctt ccacccgcga gcgaaagctc agcttcttca gcactttctt
     2581 cacgtgaacc ttcactgtgc cttcggtgat gtccagcttg cgggcgatca ttttgttgct
     2641 gtcgccggaa gcaatggctt tcagtatctg cttttcacga tcggtca
//
LOCUS       DASUEG010000920         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_610057, whole genome shotgun sequence.
ACCESSION   DASUEG010000920 DASUEG010000000
VERSION     DASUEG010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610057"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFX11_17390"
     CDS             <1..410
                     /locus_tag="VFX11_17390"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4940449.1"
                     /translation="RTLGLTLPLLGGDGINTAEFMALAGEAAEGVTASLPGVPLDSMP
                     HGTEFRQKFESKYGQIQLYAPYCYDAVMVLAAAMKKADSIEPATFLPAIAATQHDGVT
                     SQIRFDDKGDLVGGAITLYRIESGKWKTLTTIQ"
     gene            510..1439
                     /locus_tag="VFX11_17395"
     CDS             510..1439
                     /locus_tag="VFX11_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4940450.1"
                     /translation="MDIFVQQLFNGIILGSIYALIALGYTMVYGILGLINFAHGEVVM
                     IGAMVTITLLRILVEMNTGLPAAALVALAITTALPLCALLGFTLERVAYRPLRNAPRL
                     SPLITAIGLSIILQNLAMIIWGRNYAPFPPILDTTPWKILGAAITPLQVVIVILAVAI
                     MAGLWLLIGRTRKGRAMRAVAQNPNAARLMGVNVDGIISLTFVLGSALGCIAGVMVAA
                     NYGQAHATMGFMLGLKAFAAAVLGGIGNLAGAMIGGLLLGLIESFGAGYIGDLTGGFL
                     GSQYQDVFAFLVLILVLVFRPSGLMGERVAERA"
     gene            1441..>2497
                     /locus_tag="VFX11_17400"
     CDS             1441..>2497
                     /locus_tag="VFX11_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4940451.1"
                     /translation="MSKTLSVKSHSLPAKVWAGYALLALTLLIAPFIVGQVGGNAWVR
                     ILDFALLYIMLALGLNIVVGFAGLLDLGYIAFYAVGAYLYALLASAHFDYHVSAWIIL
                     PIGALLACFFGAMLGAPTLKLRGDYLAVVTLGFGEIVRLFMNNLDRPFNLTNGPQGIS
                     GIDPVSVAGYGLNKGITLFGQHFPSSYAYYYLFLVCMLLVVFVCIRLENSRLGRAWVA
                     IREDEVAAQAMGINTRNVKLLAFALGASFGGIAGGLFSGFQGFVSPESFTLMESVMVL
                     CMVVLGGMGHIPGVILGAVLLTITPEVLREVINPLQRSLFGRSVIDPENLRMVLFGLA
                     LVLVMLFRPAGLWPSQRR"
BASE COUNT          406 a          756 c          781 g          554 t
ORIGIN      
        1 tgcgcaccct cggcctcacg ctgccactgc tgggcggcga cggcatcaat accgcggaat
       61 tcatggcgct ggccggtgaa gcggcggaag gcgtgacggc gtcgctgccg ggcgtgccgc
      121 tggattcgat gccccacggt accgagttcc gccagaaatt cgaaagcaag tacggccaga
      181 tccagttgta cgcaccctac tgctacgacg ccgtgatggt gctggccgcc gccatgaaaa
      241 aagccgattc gatcgagccc gccacgttcc tgcccgccat tgccgccacc cagcacgacg
      301 gcgtgacctc gcagatccgc ttcgacgaca agggcgatct ggtaggcggc gcgatcacgc
      361 tctatcgaat tgaaagtggc aagtggaaaa ccctcacgac cattcagtga tgcgttcaca
      421 cgagggccgg ccaacaccgg ccctgcttct gtcatgacat ccggtttcac ccagcggttt
      481 caggcaacgc gattcaggta acaggctacg tggatatttt tgttcagcag cttttcaacg
      541 gcatcattct gggcagcatc tatgcgttga tcgcgctggg ctacaccatg gtgtacggca
      601 ttctggggct gatcaatttc gcccacggcg aagtggtgat gatcggcgcg atggtcacca
      661 tcaccctgct gcgcatactg gtggaaatga acactggcct gcccgccgcc gcgctggtgg
      721 cgctggccat cactaccgca ctgcccctgt gcgcactgct gggtttcacg ctggaacggg
      781 tggcgtatcg cccgctgcgc aatgcgccgc gtctgtcacc gctgattacc gcaatcgggc
      841 tttcgatcat tctgcaaaac ctggccatga ttatctgggg ccgcaactac gcgccgttcc
      901 cacccattct ggacaccaca ccctggaaaa tcctgggcgc cgccatcacg ccgttgcagg
      961 tggtgatcgt gatactggcg gtggcgatca tggcggggct gtggctgttg atcgggcgca
     1021 cgcggaaagg ccgcgccatg cgcgccgtgg cgcagaatcc gaacgcagca agactgatgg
     1081 gtgtgaatgt ggacggcatc atctcgctca cctttgtgct gggttcggcg ctgggctgca
     1141 ttgccggcgt gatggtggcc gccaattacg gtcaggccca cgccaccatg ggtttcatgc
     1201 tgggattgaa agcgtttgcg gcggcagtgc tgggcggcat cggcaatctg gccggcgcaa
     1261 tgattggcgg cttgctgctg gggctgatcg aaagttttgg cgccggttat atcggcgatc
     1321 tcaccggtgg ctttctcggc agccagtatc aggatgtgtt tgcgtttctg gtgctgatcc
     1381 tggtgctggt gttccgcccg tccggtttga tgggcgaacg cgtggcggaa cgggcctgac
     1441 atgagcaaaa cactgtccgt caaatcgcat tctttgcccg ccaaagtctg ggccggctat
     1501 gccctgctcg ctctcacatt gttgattgcg ccattcattg tcggacaggt gggcggcaac
     1561 gcctgggtgc ggattctgga tttcgcactg ctctacatca tgctggcgct ggggctgaat
     1621 atcgtcgtcg gttttgccgg cctgctggat ctgggttaca tcgcgtttta cgccgtgggc
     1681 gcctacctgt acgcgctgct ggcatcggcg catttcgatt atcacgtgtc ggcctggatc
     1741 atcctgccca tcggtgcatt actggcctgt tttttcggcg ccatgctggg ggcacccacg
     1801 ctgaaactgc gcggcgatta tctggcggtg gtcacgctcg gcttcggcga aatcgtgcgc
     1861 ctgttcatga acaacctcga ccgccctttc aacctgacca acggcccgca gggcatcagc
     1921 ggcatcgatc cggtcagcgt cgccggctac ggcctcaaca aagggatcac gctgttcggc
     1981 cagcattttc cgtcgtccta cgcgtactac tatctgttcc tggtgtgcat gctgctggtg
     2041 gtgttcgtct gcatccggct ggagaattca cgcctcggcc gcgcctgggt cgcgattcgc
     2101 gaagatgaag tcgccgcgca ggccatgggc atcaacacgc gaaatgtgaa gctgctggcg
     2161 ttcgcgctgg gtgccagctt cggcggcatc gccggcggat tgttctccgg cttccagggt
     2221 tttgtcagcc ccgaaagttt caccctgatg gaatcggtga tggtgctgtg catggtggtg
     2281 ctgggtggca tgggccatat tcccggcgtc atcctcggcg cggtgctgct gaccatcacg
     2341 ccggaagtgc tgcgcgaagt catcaacccg ctgcaacgct ctctgttcgg ccgcagcgtg
     2401 atcgatccgg aaaacctgcg catggtgctg ttcggcctgg cgctggtgct ggtgatgctg
     2461 tttcgccccg ccgggctctg gcccagccag cgtcgtc
//
LOCUS       DASUEG010000921         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_130226, whole genome shotgun sequence.
ACCESSION   DASUEG010000921 DASUEG010000000
VERSION     DASUEG010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130226"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..2155)
                     /locus_tag="VFX11_17405"
     CDS             complement(113..2155)
                     /locus_tag="VFX11_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4940452.1"
                     /translation="MGLAGQPSPDDLPTAETITVTATRIEQPISTLSNSVSLISEQDI
                     QQVSAVHASEILNRVPGVWITRGNGQEHLTAIRSPVLTGAGSCGAFYIAEEGVPVRPT
                     GFCNVNELFDLNTEQAQRIEVLRGPGTALHSSDALHGVINVISQESSQEGEGRVSVEA
                     GPNDYARIKAGTSDSQGQWGYRVNVNGATDGGYQDDSGFDQQKLTARLDYLGDEWSSF
                     SLFSASNLNQETAGYVVGTDAYKDDELRRENPNPEAYRDSQSERWQTHFERILTNDSV
                     LIITPYARYSEMEFLMHYLPGEPVEENGQTSAGVQTTYRTPVGSRLILTQGVDAEITD
                     AYLKQTQADTFSWSIPAGKQYDYEVAARLLAAFMTAEQQITDRARVSLGGRYETLEYD
                     YDNRMISGNTDGNGNPCVNTATGATGCRYTRPEDRTDDFENLSVNAGIVYHLSDAFIP
                     SARISHGFRAPQATELYRLQAGQLISNLDSEEINSAELAFKGALGAVSYDLASFYMKK
                     NNVIFQSSDRQNLDNGQTRHYGLEYQLLWQMADQWDIAAGGTFARHQYTEDVTEPGSS
                     TLLHTDGNDIVSAPHRMSSVQLGWQPVAATRLEMEWVSMGEYYTDLANLHSYEGHDLL
                     HLRLRQQVSDATNVGLRITNLTNKDYAERADYNSIVGDRYFVGQPRSVFGDVQVRF"
     gene            complement(2317..>2582)
                     /locus_tag="VFX11_17410"
     CDS             complement(2317..>2582)
                     /locus_tag="VFX11_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940453.1"
                     /translation="SELDAAVSAFCERMLNGATKAIRWTKVLINLELKRIVHSVLDTG
                     LAYEGLSAHSADHREGVRALQEKRKPVFGQQDIPGKDTPRQGD"
BASE COUNT          509 a          695 c          770 g          608 t
ORIGIN      
        1 aatgcagagt tgattctgaa agaagtgaca ggattcgaca ggaatccggt gaacctctga
       61 atgacgactg ggtatgaaaa tactgcgtta ttcagaggtt tttttatacg ccttaaaacc
      121 ggacctgcac gtcgccaaat acggatcgcg gttgcccgac gaaatagcgg tcccccacaa
      181 tgctgttgta atccgcccgt tcggcataat ctttgttggt caggttggta atgcgcaggc
      241 ccacgttggt ggcgtcgctg acctgctggc gcaatcgcag gtgcagcagg tcgtggcctt
      301 catagctgtg caggtttgcc agatcggtgt agtactcgcc catggatacc cattccattt
      361 ccaggcgcgt ggcagcgaca ggctgccagc ccagttgtac tgaactcatg cggtggggag
      421 cggaaacaat gtcgttacca tcggtgtgca gcagcgtgct ggagccgggc tcagtgacat
      481 cctcggtata ttgatgacgg gcaaaggtgc caccggccgc gatatcccac tggtcggcca
      541 tctgccagag caattgatat tccaggccgt agtggcgggt ctgtccgtta tccaggttct
      601 gccgatccga agactggaaa atcacgttgt tctttttcat atagaagctg gccaggtcat
      661 aactgacggc acccaaggcg cccttgaaag ccagttcggc gctgttgatt tcttcggaat
      721 caagattgga aatcagctgc cccgcctgca ggcgatacag ttccgtcgcc tgcggtgcgc
      781 ggaacccatg actgatccgg gcgctgggaa tgaatgcatc gctaaggtga taaacaattc
      841 ccgcgtttac cgacagattt tcaaaatcgt cggtccggtc ttccggtcgg gtgtaacggc
      901 aaccggtcgc tcctgtcgcg gtattcacgc acggattgcc attgccatcg gtattgccgc
      961 tgatcatgcg attgtcgtaa tcgtattcca gcgtttcata gcggccgccc agtgacacac
     1021 gcgcgcggtc cgttatttgc tgctccgctg tcatgaacgc cgccagcagc cgggctgcca
     1081 cttcgtagtc gtattgcttg ccggccggga tggaccagga gaaggtatcg gcctgcgtct
     1141 gcttcaggta ggcatcggtg atttcagcgt ccacaccctg ggtcagaatc aggcgggagc
     1201 cgaccggtgt gcggtaggtg gtctggactc cggcgctggt ttggccattt tcctctaccg
     1261 gttcgccggg caaataatgc atcaggaatt ccatttcgga ataacgtgca taaggggtga
     1321 tgatcagcac gctgtcgttc gtcagaatcc gttcgaaatg ggtttgccag cgctcggatt
     1381 ggctgtcgcg ataggcttcg gggttgggat tttcccggcg gagttcgtca tctttatacg
     1441 catcagtccc gaccacgtag ccggcggttt cctgattcag attgctggca ctgaagaggc
     1501 tgaagctgga ccactcgtca ccgagataat ccaggcgcgc ggtcagtttt tgctggtcga
     1561 atccggagtc gtcctgatag ccgccgtccg ttgcgccgtt gacgttgacg cgatagcccc
     1621 attgtccctg gctgtcgctg gtgccggctt tgatgcgggc atagtcattg ggaccggctt
     1681 cgacggacac ccgtccttcg ccttcctgcg aagactcctg gctgataacg ttgatcacgc
     1741 cgtgcagggc gtccgaactg tgcagcgcgg ttccagggcc gcgcagaact tcaatccgct
     1801 gggcctgctc ggtattgagg tcgaataatt cattgacgtt gcaaaagccg gtggggcgga
     1861 cgggcactcc ctcttcggcg atataaaagg cgccgcagct gccagcgcca gtcaatacgg
     1921 gcgagcggat ggcggtgaga tgttcctgac cattgccacg ggtaatccag acgcccggca
     1981 cccggtttag aatttcgctg gcatgcaccg ccgaaacctg ctgaatatcc tgttctgaaa
     2041 tcaggcttac gctgttgctc agtgtggaaa tgggctgctc gatacgagtg gctgtgacgg
     2101 tgatcgtttc tgctgtgggc aagtcgtcgg gggacggttg cccggcgaga cccatcaggg
     2161 gaattccggt aaggaacaag gcgggagtca ataaaatcca gcgtaaacgg tttttgttgt
     2221 tatcggttct ttcagtctgt gtgcgcatcg aaatacaaca ggtcactata tggggcgccc
     2281 tctgcgacaa ggcaaacagg gcgcggaaaa gcgaaatcaa tcgccctggc gtggcgtgtc
     2341 cttgcctgga atatcctgct ggccgaaaac aggtttacgc ttttcctgca gggcacgaac
     2401 gccttcgcgg tgatcggccg agtgcgcgct gagtccttcg taggccaaac cggtatccag
     2461 taccgaatgt acgatgcgct tcaattccag gttgatcaat actttcgtcc agcggatggc
     2521 tttggtggcg ccgttcagca tgcgctcgca aaatgccgat accgctgcat ccagttcgct
     2581 tg
//
LOCUS       DASUEG010000922         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1427297, whole genome shotgun sequence.
ACCESSION   DASUEG010000922 DASUEG010000000
VERSION     DASUEG010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427297"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="VFX11_17415"
     CDS             complement(<1..2257)
                     /locus_tag="VFX11_17415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940454.1"
                     /translation="MAKRFFSLKWKAFVYTSLLLILVFTVFTLTSGYFSHQRLLDLGR
                     ERTQRNVEQVDALLQRIAERTQSLARTFVNVQGVADALQRRDRDALTASVQPYWDEIG
                     ADFALRSAVVFTPEDEALMWRGDPRIPPELLEKYHQDGQAHWMLACFDLCEIYVVTPL
                     AFPGAGSDASARGMLVLSGSLQAVVSRFQDLNRSRIAISVRRNTPPETRENSLDEWGS
                     VIVSQLDQARDLPLLQAMARTYSRADVFNGVVLEADSKRFEFVLRPLRGAVFPRQGEL
                     LFVTDVTAEHVQYLAVRRSLNLFAIVGLVFAELILLSTLWSPLNRLRSIAGVLPQLAT
                     DSLGQIRRQLATLDRDHRYNDESDMLTDAALRLSHLLEDLQGEVRLRAEELEKKTIDL
                     EAEKQFVHSLLDTAHALILTQDRNGCIALINQHCTWITGFNAPELLGKSFFKLLPESE
                     QLPDLRFQLNELLNGFRNELHHESAVVRKDGSTLHMAWYHSLLPQSKEEHQILSIALD
                     ISERKEAEERLGWLASHDPLTGLFNRRRFGEELQKSIAHAKRYERTGAILFFDLDQFK
                     DVNDTSGHKVGDDLLRRVAERLRLEARDSDQIFRLGGDEFAMIVREVNRDSIAAVAKR
                     LCSALTSIEVQGSGRLHRVSSSIGIALFPEHGEYVDDLVANADIAMYQAKDAGRNGWH
                     IFSLEEQDRERIHERVYWNEKVKEALATGSLTIAFQPIQKIGATTPSHFEALLRVMDE
                     NGKPLPTYKFIQ"
     gene            2556..>3179
                     /locus_tag="VFX11_17420"
     CDS             2556..>3179
                     /locus_tag="VFX11_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4940455.1"
                     /translation="MPTVTLTPQLERIILNSQTHHHIDESGEASLAQIQRAPASSWLH
                     NPLGTINFGQSSYPYWFRTRITGLDQLPQTTYLRVDYAHLDELDIFLVRDGGIVEQYH
                     TGDTTPFSSRPIPYRTYLVPLSAQQGSSVEIYVRIATEGPIQAPMDILTRAELDRQDK
                     LNFLWFGAYFGIMIIMLFYNSLIFVFVRDLSYFLYLLYISATAFLQFT"
BASE COUNT          612 a         1035 c          901 g          631 t
ORIGIN      
        1 actggataaa tttgtaagtg ggcagcggct tgccattttc gtccatcacc cgcagcagcg
       61 cctcgaaatg gctgggcgtt gtggcgccga ttttctggat cggctggaat gcaatggtca
      121 ggctgccggt ggccagcgcc tccttcactt tttcgttcca gtacacgcgt tcgtggatgc
      181 gctcgcggtc ctgctcttcc agcgagaaaa tgtgccagcc gttgcggccc gcgtccttgg
      241 cctggtacat cgcaatgtcg gcgttggcca ccagatcatc cacgtattcg ccatgttcgg
      301 gaaacaacgc aataccgatg ctggacgaca cccggtgcaa acgcccggaa ccctgcacct
      361 cgatgctggt cagcgccgag cacagccgct tggcgacagc tgcgatggaa tcccggttca
      421 cttcacgcac gatcatcgca aattcgtcgc cgcccagccg gaaaatctgg tcggaatccc
      481 gtgcctccag tcgcagccgc tccgccaccc gccgcagcag gtcatcgccc accttgtggc
      541 cgctggtgtc gtttacgtcc ttgaactggt ccaggtcgaa aaacaggatc gcgcctgtac
      601 gttcataacg cttggcatgg gcgatggatt tctgcagctc ctcaccaaaa cggcggcggt
      661 tgaacagtcc ggtgagcgga tcgtgggacg ccagccagcc cagccgttcc tcggcttcct
      721 tgcgctcgct gatgtccagg gcaatgctga gaatctggtg ttcttccttt gattgcggca
      781 gcagcgagtg gtaccaggcc atgtgcagcg tggagccgtc cttgcgcacc accgccgatt
      841 cgtgatgcag ctcgttgcgg aagccgttga gcagttcgtt cagctggaaa cgcagatcgg
      901 gcaactgctc gctttccggc agcagtttga agaaggattt gcccagcagt tcaggcgcgt
      961 tgaagccggt gatccaggtg cagtgctggt tgatcagggc gatgcagccg ttgcggtcct
     1021 gggtcaggat cagggcgtgg gcggtatcca gcaggctgtg cacaaactgc ttttccgctt
     1081 ccaggtcgat ggttttcttt tccagttcct cggcgcgcaa ccgcacttcc ccctgcaggt
     1141 cttccagcag atgggacagg cgcagcgcgg catccgtcag catatcgctt tcgtcattgt
     1201 aacggtggtc gcgatccagt gtggccagct ggcgccggat ctggcccaga ctgtcggtgg
     1261 ccagctgcgg cagcacgccg gcaatggaac gcagccggtt cagcgggctc cacagggtgg
     1321 acagcaggat cagttcggca aacaccaggc cgacgatggc aaacaggttg agactgcgac
     1381 gcaccgccag atactggacg tgttcggctg tcacgtccgt cacgaacaac agctcgccct
     1441 ggcggggaaa aacggcgccc cgcaggggcc gcaacacaaa ttcgaagcgt ttgctgtccg
     1501 cttccagcac cacgccgttg aacacgtcgg cgcgggaata ggtccgggcc atggcctgca
     1561 acagcggcag atcccgcgcc tgatccagtt gcgacacgat gacactgccc cactcgtcca
     1621 ggctgttctc gcgggtttcc ggcggcgtat tacgccgcac cgaaatcgca atgcgggacc
     1681 ggttcagatc ctgaaagcgg ctcaccaccg cctgcaggga accgctcagc accagcatgc
     1741 cccgggcgga cgcgtcgctg ccggcgccag gaaatgccag cggtgtcacg acgtagattt
     1801 cacacagatc aaagcacgcc agcatccagt gcgcctggcc gtcctgatga tatttttcca
     1861 gcagttccgg tggaatgcgc ggatcaccgc gccacatcag ggcttcgtcc tccggggtga
     1921 acaccaccgc cgaacgcagg gcaaaatcag cgccgatttc gtcccagtag ggctgcacac
     1981 tggccgtcag cgcgtcgcgg tcccgccgct gcagcgcatc cgccacaccc tggacattga
     2041 caaacgtgcg cgccagggac tgggtacgct ccgcgatccg ctgcagcagg gcgtccacct
     2101 gctccacatt gcgctgggta cgctcacgac ccagatccaa caatcgctgg tgcgaaaaat
     2161 agccagatgt cagcgtgaag accgtgaaca ccagaatcag caacaggctg gtatagacga
     2221 aggccttcca cttaagactg aaaaatcgtt ttgccacttg taggggtact caccctttcc
     2281 cggttcgctc ctaaccagta tagagaggga ttcgcccgat cacccccgaa ccggaaagct
     2341 aatcaatcta ggctaattga cagccctatt cactcaacta ggcttttacg gagtacccac
     2401 ctgcttttcc gcgtccggag gaatggcttc tgtcttcgcg tacccaggcc ctgccctctt
     2461 ctgcccgacg tcctgcccgt ccgcctcgcg cgctttggct ggtctgcctg ctgctgtggt
     2521 gctgcctggc acgggcggca gataatccag ccgacatgcc aacggtgacc ctgacgccgc
     2581 agctggagcg gatcatcctg aacagccaga cccatcacca catcgacgaa tccggcgagg
     2641 cctcgctggc ccagatacag cgggcaccgg ccagcagctg gctgcacaac cccctgggta
     2701 ccatcaattt cggccagtcc tcctatccct actggttccg cacccgcatc accggcctgg
     2761 accagctgcc acaaaccacc tacctgcgcg tcgactacgc ccacctggac gaactggata
     2821 tcttcctggt acgcgacggc ggcattgtgg agcagtacca caccggtgac accaccccgt
     2881 tttccagccg tccgattccc tatcgcacct atctggttcc gttaagcgcg cagcagggaa
     2941 gcagcgtgga aatctacgtg cgcatcgcca ccgagggccc gatccaggcg ccaatggaca
     3001 tcctcacccg tgcggagctg gaccggcagg acaaactgaa tttcctctgg ttcggcgcct
     3061 atttcggcat catgatcatc atgctgttct ataacagcct gatcttcgtg ttcgtgcgcg
     3121 atctgagtta tttcctttac ctgctgtaca tcagtgccac ggcgttcctg caattcacc
//
LOCUS       DASUEG010000923         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_788301, whole genome shotgun sequence.
ACCESSION   DASUEG010000923 DASUEG010000000
VERSION     DASUEG010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788301"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFX11_17425"
     CDS             complement(<1..559)
                     /locus_tag="VFX11_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940456.1"
                     /translation="MDAVIPCPESGAAAPARTLPARWLLHRHAARWITALATLLALPS
                     LWSGLVADDYYHAARFLAPGLLPDIGSYSLFDLFAVSDGASATNTRLVEQGLLPWWTD
                     SQFRFQLWRPLAEASHWLDYRLWPSSPWLMHLHQLAWFAALQLVVLLFYRRLFPAQAL
                     GVLAFALFVLSANFSQTVTWLAARNT"
     gene            796..2796
                     /gene="tkt"
                     /locus_tag="VFX11_17430"
     CDS             796..2796
                     /gene="tkt"
                     /locus_tag="VFX11_17430"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX4940457.1"
                     /translation="MPTRSELANAIRALSMDAVQKAKSGHPGAPMGMADIAQVLWCDY
                     LKHNPSNPKWPDRDRFILSNGHGSMLIYSLLHLTGYDVSIDDLKNFRQLHSKTPGHPE
                     YGYTPGVETTTGPLGQGIANAVGMAIAEKALAAHFNKGGYNIVDHNTYVFMGDGCMME
                     GISHEVCSLAGTLGLGKLIAFYDDNGISIDGEVHGWFTDNTPKRFESYGWHVIPNVNG
                     HNPDEIRAAIEMARANADRPSLICCKTIIGYGAPNKQGKEECHGAPLGDDEIKLARAQ
                     LGWNHAPFEVPSDIYAEWNAKEKGARAESDWQRQFDNYRKEFPQLAAEFERRMKGELP
                     ADWAEKSAAYIQQVQAGGEKIASRKASQNSLNAFGPLLPELLGGSADLAGSNLTIWKG
                     CKGISAEDAAGNYVYWGVREFGMTAAMNGIALHGGFITYGATFLIFMEYARNAMRMAA
                     LMKQQNIQVYTHDSIGLGEDGPTHQPVEQLANLRTTPNMSLWRPCDAAESAVAWKAAI
                     ERRNGPTALVFSRQNLAPMPRTAEQLANVAKGGYVLKAFSAGKQDALIIATGSEVELA
                     VKAAETLAAKGRNVSVVSMPSVDVFLQQDAAYRESVLPNAVRARVAVEAGIADYWNKF
                     VGLDGAVVGMTTFGESAPADLLFKQFGFTVENVVAKVESVLR"
     gene            2798..>3354
                     /locus_tag="VFX11_17435"
     CDS             2798..>3354
                     /locus_tag="VFX11_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX4940458.1"
                     /translation="MTLRIAINGYGRIGRTMLRALYEGRAHGCNRDIQLVAINDLGAA
                     ESLLHLTKYDTTHGRFHTPVTLLDDNRLQVNGDQILLLQIADPAQCPWAELDIDVVLE
                     CTGRFRSHAAASRHLQAGAKRVVIGAVAFDDVDATIVFGVNHEELRPEHKIISAASCT
                     THCIAPLLQLMDSTFGVKQAFMTEI"
BASE COUNT          723 a         1042 c         1021 g          568 t
ORIGIN      
        1 gggtgttgcg cgccgcaagc caggtgacgg tctggctgaa attggccgac agcacaaaca
       61 gcgcaaaggc cagaacgccc agcgcctgcg ccggaaacag ccgccggtag aacagcagca
      121 caaccagctg cagggcggca aaccaggcca gctgatgcag gtgcatcagc cagggtgagg
      181 aaggccacaa ccggtaatcg agccagtgac tcgcttccgc cagcggccgc cacagctgga
      241 accggaactg gctgtcggtc caccagggca gcagtccctg ctccaccagc cgggtattcg
      301 ttgcactggc gccatcgctc accgcaaaca ggtcgaacag cgagtagctg ccaatgtccg
      361 gcaacagacc tggcgccaga aagcgtgcgg cgtgatagta gtcatcggcc accagtccgc
      421 tccacaggga cggcagcgcc agcagggtgg ccagcgccgt gatccagcgc gcggcatgcc
      481 gatgcagcag ccagcgggcc ggcagggtac gagcgggcgc ggcagcgccg ctttcagggc
      541 agggaatgac agcgtccatg gggctatctt gttgtgttta taggggaatc aggcgcgaat
      601 gagcgttggt gccgcagttg taaatgcatc ccgccacggc aacaagtgcg gcgggatcga
      661 atttacacaa atccaacaca gatccccgcg cagcgcgggc gccagcggcg atcccgtttg
      721 ccgctcatgc ctacttgagc gacaatagcg cccccaaaac cgcgacccgg actaaatcag
      781 ttaggagcat ttccgatgcc cacccgcagt gaacttgcca atgccattcg cgccctctcc
      841 atggacgcag tccagaaagc caagtccggt cacccgggcg ccccgatggg aatggctgat
      901 atcgcgcagg tcctgtggtg cgactacctg aagcacaacc cgtccaaccc gaaatggccc
      961 gaccgcgacc gcttcatcct gtccaacggt cacggttcca tgctcattta ttccctgctg
     1021 cacctgaccg gttatgacgt ctccatcgac gatctgaaaa acttccgcca gctgcactcg
     1081 aaaacccccg gtcaccccga atacggctac acccccggtg tggaaaccac caccggaccg
     1141 ctgggacagg gcattgccaa cgcggtgggc atggcgatcg cagaaaaagc gctggcggca
     1201 cacttcaata aaggcggcta caacatcgtc gaccataaca cctacgtgtt catgggcgac
     1261 ggctgcatga tggaaggcat ttcccacgaa gtctgctcgc tggcgggcac actgggcctg
     1321 ggcaagctga tcgcgttcta cgacgacaac ggcatctcca tcgacggcga agtgcacggc
     1381 tggttcaccg acaacacgcc caagcgtttt gaaagctacg gctggcatgt aattccgaat
     1441 gtgaacggtc acaatccgga cgaaatccgc gccgccatcg aaatggcccg cgccaatgcc
     1501 gatcgtccgt cgctgatctg ctgcaagacc atcattggct acggcgcccc caacaaacag
     1561 ggcaaggaag aatgccacgg tgcgccgctg ggcgacgacg aaatcaagct ggcccgcgcg
     1621 cagctgggct ggaatcacgc gccgtttgaa gtgccgtccg atatttacgc cgaatggaat
     1681 gcgaaagaaa aaggcgcccg cgcggaaagc gactggcagc gccagttcga caactaccgc
     1741 aaggaattcc cgcaactggc ggcggaattc gaacgccgca tgaaaggcga actgccggcg
     1801 gactgggcgg aaaaatccgc cgcctacatt cagcaggtgc aggcgggcgg cgagaaaatt
     1861 gcctcgcgca aagcgtcgca gaattcactc aatgcgtttg gtccgctgct gccggaactg
     1921 ctgggcggtt ctgccgacct ggccggttcc aacctgacca tctggaaagg ctgcaagggc
     1981 atttccgctg aagacgcagc cggcaactac gtttactggg gtgtgcgcga attcggcatg
     2041 accgccgcca tgaacggcat cgcactgcac ggcggtttca tcacctacgg cgccactttc
     2101 ctgatcttca tggaatacgc gcgcaacgcc atgcgcatgg ccgcgctgat gaagcagcag
     2161 aacattcagg tctacaccca cgactccatt ggcctgggcg aagacggccc cacccaccag
     2221 cctgtcgagc agctggccaa cctgcgcacc acgcccaaca tgtcgttgtg gcgtccgtgc
     2281 gatgctgccg agtccgctgt ggcctggaag gcggcgatcg aacgtcgtaa cggccccact
     2341 gcactggtgt tctcgcgcca gaatctggca ccgatgccgc gcacggccga gcaactggcg
     2401 aacgtggcaa agggtggtta tgtgttgaaa gcgttctccg ccggcaaaca ggacgcgctg
     2461 atcattgcca ccggctccga agtggaattg gcggtgaaag cagccgaaac actggctgcg
     2521 aaaggcagga acgtgtcggt ggtgtccatg cccagcgtcg atgttttcct gcagcaggac
     2581 gcggcctatc gcgaatccgt actgcccaac gctgtgcgcg cccgcgtggc ggtggaagcc
     2641 ggcattgccg attactggaa caagtttgtt ggcctcgacg gcgccgtagt gggcatgacc
     2701 acgttcggcg aatccgcacc ggcggacctg ctgttcaagc agttcggttt cactgtggaa
     2761 aatgtagtgg cgaaagtgga gtcggtcctc cgttgatatg actctgcgca tcgccatcaa
     2821 cggctacggc cgtatcggac gcaccatgct gcgcgcgctg tatgaaggcc gcgcccacgg
     2881 ttgcaatcgc gatatccagc tggtggcgat caatgatctg ggtgcagcgg aatccctgct
     2941 gcacctgacg aaatacgaca ccacccacgg ccgtttccac acgccggtca ccttgctgga
     3001 cgacaatcgg ctgcaggtga acggcgacca gatcctgcta ttgcagatag ccgatccggc
     3061 gcaatgtccg tgggcggaac tggatatcga tgtggtgctg gaatgcaccg gccggtttcg
     3121 ttcgcacgcg gctgccagcc ggcatctgca ggcgggtgcg aagcgggtgg tgatcggtgc
     3181 ggtggcattc gatgatgtgg acgcgacgat tgtattcggc gtgaatcacg aggaattgcg
     3241 gccggagcac aagatcattt cggcagcgtc ctgcaccacg cactgcatcg caccgctgct
     3301 gcagttgatg gattcgacct tcggcgtgaa acaggcgttc atgaccgaaa tcca
//
LOCUS       DASUEG010000924         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1612479, whole genome shotgun sequence.
ACCESSION   DASUEG010000924 DASUEG010000000
VERSION     DASUEG010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612479"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1900)
                     /gene="purH"
                     /locus_tag="VFX11_17440"
     CDS             complement(284..1900)
                     /gene="purH"
                     /locus_tag="VFX11_17440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX4940459.1"
                     /translation="MPLREPSHSSITRQTAIMSQPIKRALISVSDKTGIVDFARALSA
                     MGVEILSTGGTYKLLREQGVKAVEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGIDD
                     AAMQQHGITPIDLVAVNLYPFEQTVAKPDCDLETAIENIDIGGPTMVRSSAKNHKWVT
                     IVVNASDYGRVLDEMQAGGNVVSDRTRFELALAAFEHTAKYDGMIANYLGSLQNDGSK
                     TPFPRTFNMQFEKVQEMRYGENPHQRAAFYREARPAEPSVSTAKQLQGKELSFNNVAD
                     TDAALECVKSFAEPACVIVKHANPCGVAIGANINEAYERAFLTDPTSAFGGIIAFNQP
                     LDEKTARNIVDKQFVEVIIAPGASNAAIAIVGEKKNVRLLVCEPWGKRVDGFDYKRVN
                     GGLLVQDRDNGMITQSDLKLVTKRAPSAEEMRDLLFCWQVAKFVKSNAIVYAKNLQTI
                     GIGAGQMSRVYSAKIAGIKAEDEGLVVPGSVMASDAFFPFRDGIDAAAKAGIRAVIQP
                     GGSIRDEEVIAAADEADIAMVFTGMRHFRH"
     gene            complement(2062..2397)
                     /gene="fis"
                     /locus_tag="VFX11_17445"
     CDS             complement(2062..2397)
                     /gene="fis"
                     /locus_tag="VFX11_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEX4940460.1"
                     /translation="MNETNYRDDDESYASNATSASSAELKQHLVTPVEQGNQTIRDNV
                     HKALRNYFAHLDGQPVTDVYGMVLSEVEAPLLETVMEYTRGNQTKASILLGLNRGTLR
                     KKLKTYGLI"
     gene            complement(2394..3386)
                     /gene="dusB"
                     /locus_tag="VFX11_17450"
     CDS             complement(2394..3386)
                     /gene="dusB"
                     /locus_tag="VFX11_17450"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003217321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX4940461.1"
                     /translation="MSPRIGPYALKSPVILAPMAGVTDQPFRLLCMRLGAGMVVAEML
                     TSDTSLWQSRKSSLRLIHQDEPEPRSVQIAGNEPDMMAEAARLCVERGAQIVDINMGC
                     PAKKVCKKAAGSALLKDEVLVRSILKAVVAAVDVPVTLKIRTGWSPDQRNGLTIARIA
                     EDTGIQALAVHGRTRACGFKGEVEYDTIAQIKEAVSIPVFANGDITTPLQAVQILRST
                     GADGIMIGRAAQGNPWIFREIAHFLESGETLPAPAYSEMQAITVEHLHRLHDFYGAHL
                     GVRIARKHLGWYVQHLPLGAQFRSRFNTLETPADQLASAQDYFEQLIEGEVMAA"
     gene            complement(3566..>4180)
                     /locus_tag="VFX11_17455"
     CDS             complement(3566..>4180)
                     /locus_tag="VFX11_17455"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX4940462.1"
                     /translation="LEQKEPEEEITPMKLFQDSDDVINRQVRLSTLQFGEDEPSRQPL
                     RTALMLLGSLLLVMALAAQYVYFNLDDVARNPTLRPWLEMVCKPAGCTLPAQSDLARI
                     VSANLVVRSHPHERNALVIDVIIKNTAPFEQPFPAVQLTFEDINNNPVASRRFLPAEY
                     IHDTSVDIQRMPANTPIHLTLEIVDPGKNAVNYQLTFLPVDGKK"
BASE COUNT          801 a         1190 c         1242 g          947 t
ORIGIN      
        1 gctgacgttc cggaaaaccc acctcggacc aacggagcta cgtgccacag taaagctcgg
       61 tttgctcttc tggtcagaga gagtaattcc agaagctggc acaatgtctg acaagcagga
      121 tcgggtcata gggttcaagg acgtcagcgg ccatggatgg ccgctgtcga gcctacaggg
      181 atgtacttgt ggcgtgcctt gaaccctatg acccgatcct gcgctcgcac caaactgtat
      241 tgaactctgg attgggccac gaagaaacga cccgaaccaa aaatcagtgc cggaaatgcc
      301 gcatcccggt aaacaccatc gcaatgtccg cctcgtccgc tgccgcaatc acttcctcgt
      361 cgcgaatcga accgccgggc tggatcaccg cacgaatgcc tgccttcgcc gccgcatcaa
      421 tgccatcgcg gaacgggaaa aacgcatccg atgccatcac cgatcccggc accaccaggc
      481 cttcgtcttc cgccttgatg ccagcgatct ttgcactgta cacccggctc atctggccgg
      541 caccgatacc gatggtctgc agattcttcg catacacaat cgcattggat ttaacaaatt
      601 tcgcaacctg ccagcagaac agcagatcgc gcatttcctc ggcggacggc gcgcgcttcg
      661 tcaccagttt cagatcggat tgcgtgatca tgccgttgtc acggtcctgc accagcagac
      721 cgccgttcac gcgcttgtaa tcgaaaccat cgacacgctt gccccagggc tcgcagacca
      781 gcagacgaac attcttcttc tcgcccacaa tcgcaattgc tgcattgctc gcgcccggcg
      841 cgatgatcac ttccacgaac tgcttgtcga cgatattgcg ggcggttttc tcatccagcg
      901 gctggttgaa ggcaatgatg ccaccgaatg cagaggtggg atccgtcagg aacgcgcgct
      961 cgtaggcttc gttgatattc gcgccgatgg ccacgccgca ggggttggcg tgcttgacga
     1021 tcacacacgc gggctcggca aacgacttca cgcattccag cgcggcgtca gtatctgcca
     1081 cgttgttgaa tgacagttcc ttgccctgca actgcttggc cgtgctcacg ctgggctcgg
     1141 cgggcctggc ttcgcggtag aacgcagcgc gctggtgcgg gttctcaccg tagcgcattt
     1201 cctgcacttt ttcgaattgc atgttgaagg tgcgggggaa gggcgtcttg ctgccgtcgt
     1261 tctgcaggct gcccagataa ttcgcgatca tgccatcgta tttggcagtg tgttcgaacg
     1321 cagccagcgc cagttcgaaa cgggtgcggt cactcaccac attgccaccg gcttgcattt
     1381 catccagcac gcggccgtaa tcggacgcgt tcaccacaat ggtgacccac ttatggttct
     1441 tggcagagga gcgcaccatg gtcgggccgc cgatatcgat attctcgatc gccgtttcca
     1501 gatcgcagtc cggcttggcc accgtctgtt caaacggata caggtttact gccaccaggt
     1561 cgatcggcgt gatgccgtgc tgctgcatgg ccgcgtcatc gatgccgcgg cggcccagaa
     1621 tgccgccgtg cactttcggg tgcagggttt tcacgcggcc gtccatcatt tccggaaagc
     1681 cagtgtagtc ggagacttcc accgccttca cgccctgttc ccgcagcagc ttgtaagtgc
     1741 cgccagtaga aaggatttcg acgcccatgg cgctcagggc gcgggcaaag tcgacgatac
     1801 cggttttgtc ggaaacactg attaatgcgc gtttgatggg ctggctcatg atggctgtct
     1861 gtcgcgtaat gctggagtgg gatggttcgc gcaggggcat ggccgccggc gaggggcgcc
     1921 attgtagtcg gaatcggggc cggatttaag acggaggcca aaaaaacacc aaggcggtgt
     1981 caaaccgatt gcctgtcgct gaatacatca acagcggctt tccctcgcag gagggatggg
     2041 ccgctgttgc cgccagactg atcagatcag tccgtaggtt ttcagcttct tgcgcagggt
     2101 gccgcggttc agtccaagca ggatggatgc cttggtctga ttgcccctgg tgtactccat
     2161 aacggtttcc agcaggggtg cctcaacctc ggacagaacc atgccataca catccgttac
     2221 cggctggccg tccaggtggg caaagtagtt acgcagtgcc ttgtggacgt tgtcgcggat
     2281 ggtctggttg ccctgttcaa ccggcgtcac cagatgctgc ttgagttccg cgctgctggc
     2341 tgaagttgcg ttgctggcgt agctttcgtc gtcgtctctg taatttgttt cgttcatgcc
     2401 gccattacct ctccctcgat tagttgctca aagtaatcct gagcactggc gagttgatct
     2461 gctggtgttt caagcgtgtt aaaacgtgac ctgaattgtg ctcccaacgg caaatgttgt
     2521 acataccagc ccaggtgttt tctggctata cgtacgccca gatgcgcgcc ataaaagtca
     2581 tgcagacggt gcagatgctc caccgtaatt gcctgcattt ccgagtatgc cggtgccgga
     2641 agcgtttccc ccgattcgag aaaatgggcg atctctcgaa agatccaggg attgccctgt
     2701 gctgcccgcc cgatcatgat gccatcggcg ccggtgctgc ggaggatctg cactgcttgg
     2761 aggggagtgg tgatgtcgcc attggcaaat accgggatac tgacggcttc cttgatttgc
     2821 gcaatggtgt cgtactccac ctcgcccttg aacccgcagg ctcgtgtcct tccatgaact
     2881 gccagtgcct gaatgcccgt gtcttccgcg atgcgggcaa tggtgaggcc attgcgctga
     2941 tcgggtgacc atccggtacg aatcttcagg gtgaccggta catccacagc cgccacaacg
     3001 gctttcaata tggatctgac cagtacttca tctttgagca aggccgaacc tgctgctttc
     3061 ttgcaaacct tcttggcggg gcagcccatg ttgatgtcaa cgatctgggc gccacgttcc
     3121 acacaaagcc gggctgcttc tgccatcatg tcgggttcgt tgccggcgat ctgcactgag
     3181 cgcggctcgg gctcgtcctg atggatcagg cgcaggctgg atttgcggga ttgccacagg
     3241 ctggtatcgc tggtcagcat ttccgctacc accatgccgg cgccgaggcg catgcagagg
     3301 agtcggaagg gctggtcggt gacgccggcc atgggtgcaa gtatcactgg gcttttcagt
     3361 gcgtagggcc cgattcgagg ggacattcga actcccgatc aataaaacgc ctaatgacat
     3421 tcagccgtgc ttaaagatcg agctatcata gcgggaagat ggggcggaat aaacagggtt
     3481 ttaaatggat ttgtaaaatt acccgactgt tggtgaatgt tcagccacgg gacggcgcca
     3541 gttgtgtcaa aaacctacaa tctgtctatt ttttgccgtc cacgggcaga aatgtcagct
     3601 gataattgac ggcgtttttg cccgggtcga cgatttccag ggtcaggtgg atgggcgtgt
     3661 tggctggcat gcgctggatg tcgacgctgg tgtcgtgaat gtactcggct ggcaggaagc
     3721 gccggctggc gaccgggttg ttgttgatgt cctcgaaggt gagctgcacc gcggggaagg
     3781 gctgttcgaa tggtgcggtg ttcttgatga tcacgtcgat caccagggcg ttgcgctcat
     3841 gggggtggga acggaccacc aggttggcgc tcacgatgcg ggcaagatcg ctctgggctg
     3901 gcagggtgca gccggcgggc ttgcacacca tttccagcca gggtctcagg gtcgggttgc
     3961 gcgccacgtc gtccaggttg aaatagacat actgggctgc cagggccatg accagcagga
     4021 gtgagcccag cagcatgagg gcggtgcgca gcggctggcg cgatggctca tcctcgccaa
     4081 actgcagggt gctgaggcgc acctgccggt tgatgacgtc gtcactgtcc tggaacagct
     4141 tcatcggtgt gatttcttct tccggttctt tctgctccag
//
LOCUS       DASUEG010000925         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1294860, whole genome shotgun sequence.
ACCESSION   DASUEG010000925 DASUEG010000000
VERSION     DASUEG010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294860"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..852)
                     /locus_tag="VFX11_17460"
     CDS             complement(115..852)
                     /locus_tag="VFX11_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940463.1"
                     /translation="MGNNYSTPSASLVNTTGNPDALQAGIAGDYQLAIGDVISEAWQR
                     TKGIKRYVLGAGILLYILMFVFIMILGVIMALTGQTGEEISAASVVLQFVVQIALMAV
                     ILPFVGGIFIMCIKHIQGHQVSFGDLFSCLGKTGSLLITAILMNIMIFIGFMLFVLPG
                     IYLSVAYILTVPLVVDRGLGPWEALEASRKAITKKWFTVLGFYLVLLVIMVVSMIPLG
                     LGLIWTIPMMAVAFAILYRQVFGIESY"
     gene            complement(970..2334)
                     /gene="chrA"
                     /locus_tag="VFX11_17465"
     CDS             complement(970..2334)
                     /gene="chrA"
                     /locus_tag="VFX11_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX4940464.1"
                     /translation="MPSSSTPSALADAAPPAPISFWQAFRFWLKLGFISFGGPAGQIA
                     IMHSELVERQRWLSERRFLHALNYCMVLPGPEAQQLATYIGWLMHRSWGGIVAGGLFV
                     LPSLLILIVLSWIYIAFGELPLVAGLFYGIKPAVTAIVVQAAHRIGTRALKNNILWGI
                     AAASFVAIFALNVPFPAIVLGAALIGYLGGRLMPDHFKVGGGHGSAGKSYGPAIIDDT
                     TPPPAHTRFRWPRLVALLVIGAALWLLPMAALTLSLGWDHTFTQMGWFFTKAALLTFG
                     GAYAVLPYVYQGAVTHYGWVTPTQMIDGLALGETTPGPLIMVVAFVGFVGGYVKSLFG
                     PDALFLAGAAAATLVTWFTFLPSFLFILIGAPLVESTHGDLKFTAPLTAITAAVVGVV
                     INLALFFGYHVLWPQGFAGAFDGISAAIALAATVALFRFKRTVMEVIAACAGVGLMVT
                     LLLR"
     gene            complement(2418..3032)
                     /locus_tag="VFX11_17470"
     CDS             complement(2418..3032)
                     /locus_tag="VFX11_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX4940465.1"
                     /translation="MPHSPKPALSAILRTLADDTRLERVAISDLLAALGDRAIGALLF
                     VFAFPNVLPAPPGLSGILGAPLIFLAAQLTLGLKPWLPEIITRRSMARQDFQALIQRM
                     APWLERAEKLLRPRLITATLPPMEYLVGLVCLLMACVLVLPIPLGNILPALGISLMSL
                     GVVARDGVWVMLGLAVAVSASVVVSGVVFALLKAALFLFTEILQ"
     gene            complement(3085..4218)
                     /locus_tag="VFX11_17475"
     CDS             complement(3085..4218)
                     /locus_tag="VFX11_17475"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX4940466.1"
                     /translation="MDDPLHIDRWTGLNDANSIGGKLQFMHGVMQTHMPFVTRLAVAL
                     YDADTDDLRTFAFSSREASPLTHYSAKLASCHSLHNIITSGQPRVVNDLSIYNDVDHE
                     HTRVIYASGYRSSYTLPMVWEGNFFGFVFFNSHLTDVFTNRVLNELDVIGHMIALLIY
                     NERTNVRTMLATIRSALDLTHSRDPETGCHLERMSRYARLIAKNLASRFGFDDQYIEH
                     VFLFAPLHDLGKLAIPDRILLKEGPLDDAEYSIMRTHSREGRRMIDKLLLNYGLNGVT
                     HVDMLRNIALHHHEAVDGSGYPEGLAGDQIPIEARIVAVADVFDALTSRRPYKPAWTN
                     AAAFAKLRSLAGVKLDADCVSALIDNEQPLLEIQAAFQENPYG"
     gene            4384..>5073
                     /locus_tag="VFX11_17480"
     CDS             4384..>5073
                     /locus_tag="VFX11_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX4940467.1"
                     /translation="MSSLRTEQDSLGPVEVPADCLWGAQTQRSLEHFAISTERMPDEF
                     IQALAAVKLACTKVNRDLGLLPESHANAIMDAAGEVMIGQHFREFPLSIWQTGSGTQT
                     NMNMNEVLANRASELLGGERGELRQVHPNDQVNLGQSSNDVFPTAMHLAAARGVFLLL
                     LPALSVLQKALAAKSAAFADIIKIGRTHLQDATPLTLGQEFSGYAAQLDQARLLIEQT
                     LPAIYPLAIGGT"
BASE COUNT         1082 a         1518 c         1560 g          913 t
ORIGIN      
        1 gattgccgcg gatgctgtaa atgcattgcc ctgtctcggc tgaaagcgaa taggccgtaa
       61 aaagaaaagg ccccggtttc ggggcctttt gtgttctgct gcagacgatc gctctcagta
      121 gctttcgatc ccgaacacct gccggtacag gatcgcaaat gccaccgcca tcatcgggat
      181 ggtccagatc aggccaagtc ccagcggaat catgctcacc accatgatca ccagcagcac
      241 gagataaaag ccgagcacgg tgaaccactt cttggtgatc gccttgcgcg aggcttccag
      301 cgcttcccac gggcccagac cgcggtccac caccagcggc acggtcagga tataggccac
      361 cgacagatag atgccgggaa ggacgaacag catgaatccg atgaagatca tgatgttcat
      421 caggatcgcg gtgatcagca ggctgccggt tttgccgagg caggaaaaca gatcgccaaa
      481 gctgacctgg tgtccctgga tatgcttgat gcacatgatg aagatgccgc cgacgaaggg
      541 caggatcacc gccatcaagg cgatctgcac cacgaattgc agcacaacgc tggcggcgga
      601 gatctcttcg ccggtctgac cggtcagcgc catgatgacg cccaggatca tgatgaatac
      661 gaacatcagg atatacagca gaatgcctgc gcccagcaca taacgcttga ttcctttcgt
      721 tctctgccag gcttcggaaa tcacatcgcc gatcgccagc tggtagtcac cggcaatgcc
      781 cgcctgcagc gcgtcgggat tacctgtcgt gttcaccagc gacgctgaag gggtggaata
      841 gttgttgccc actggaaact ccttgtcgtt gttttttgaa aatgcggttg atcgccgttt
      901 cagtatagcg gggaacgcct tcatttccgg cgtgggagtt ttgtgcactt tttcacttca
      961 ggcatggcat caccgcaaga gcagcgtcac catcagcccc acgccggcgc aggcggcgat
     1021 cacttccatg accgtgcgct tgaagcggaa cagtgcgacg gtggccgcca gggcgattgc
     1081 agcggatatc ccgtcgaacg cgcccgcgaa tccctgcggc cacagcacgt gatagccgaa
     1141 gaacagcgcc aggttgatga ccacgcccac cacggcagcg gtgattgccg tgagcggtgc
     1201 ggtgaatttc aggtcgccgt gggtggattc aacgaggggg gcgccgatca ggatgaacag
     1261 gaacgagggc aggaacgtga accaggtaac caatgttgct gctgccgcgc cggccagaaa
     1321 aagtgcgtcc gggccgaaca gggatttcac atagccgcca acaaagccga cgaaggccac
     1381 caccatgatc agcggtcccg gcgtggtttc ccccagggcc aggccgtcaa tcatttgcgt
     1441 cggcgtcacc cagccgtaat gcgtcactgc accctggtac acatagggca gcaccgcata
     1501 ggcgccaccg aaggtgagca gcgccgcttt cgtaaagaac catcccatct gggtgaaggt
     1561 gtgatcccag cccaggctca gggtgagtgc ggccatgggc agcaaccaca gcgccgcgcc
     1621 gatcaccagc agggccacca gtcggggcca gcggaagcgg gtgtgggcgg gcggcggcgt
     1681 ggtgtcgtcg atgatggccg ggccgtagga tttgccggca ctgccgtgac cgccgccgac
     1741 cttgaagtgg tccggcatca ggcgcccgcc cagatagccg atcagggcgg cccccagcac
     1801 gatagcggga aacggtacgt tcaacgcgaa tatcgccacg aacgaggcgg ccgcgattcc
     1861 ccacagaata ttgtttttca gcgcccgggt gccgatgcgg tgcgccgcct gcaccacgat
     1921 ggcggtgacc gccggcttga tgccataaaa cagccccgcc accagtggca gttcgccgaa
     1981 cgcaatgtaa atccaggaca gcacgatcag gatcagcaac gacggcagca cgaacagtcc
     2041 ccccgccacg ataccgcccc agctgcggtg catcagccag ccgatatagg tggccagctg
     2101 ttgcgcttcc ggcccaggca acaccatgca gtaattcagt gcatgcagga agcgtcgctc
     2161 cgacagccag cgctgccgtt ccaccagctc gctgtgcatg atggcgattt gccccgcagg
     2221 cccgccaaaa ctgatgaacc ccagcttgag ccagaaacgg aatgcctgcc agaaactgat
     2281 cggggcaggt ggagcggcgt cggcgagggc ggatggggtg gacgatgacg gcattcaacg
     2341 aggctcccgc tcagaagaca aaggtgaggg tccagtgtaa cccgcttcaa cggcaggtta
     2401 atgcaggcga gccgcgttca ctgcaggatt tcagtgaaca ggaacagcgc cgctttcagc
     2461 agagcaaaca ccacgccaga caccacgacg gaagcagaca cggccacggc cagaccgagc
     2521 atcacccaca caccgtcccg cgccaccacg cccaggctca tcagggaaat gcccagtgcc
     2581 ggcaggatat tgcccagcgg aatcggcagc accagtacgc acgccatcag cagacacacc
     2641 agccccacca ggtattccat cggcggcagc gtggcggtga tcaggcgtgg acgcagcagt
     2701 ttttccgccc gctccagcca gggcgccatg cgctggatca gggcctggaa atcctgccgg
     2761 gccatggaac gccgggtgat gatctccggc agccagggct tgagaccgag tgtgagctgg
     2821 gccgccagaa aaatcagcgg cgcgccgaga atgcccgaca atcccggtgg cgccggcagc
     2881 acattgggaa aggcgaacac gaacagcaat gcgccgatgg cgcggtcacc cagggcggcg
     2941 agcaggtcgc tgatggcgac ccgttccagg cgcgtgtcat cggcgagtgt gcggagaatc
     3001 gcggaaaggg cgggtttggg gctgtggggc atggtgcggt tccggttact gctggttcag
     3061 gggcactagc ttacgctatt ggtttcatcc gtagggattt tcctggaatg cggcctggat
     3121 ctccagcagc ggctgttcgt tatcgatcaa cgcgctgacg cagtccgcat ccagcttgac
     3181 ccccgccagg gaacgcaact tggcgaaggc cgccgcgttg gtccaggctg gcttgtaggg
     3241 gcgccggctg gtgagggcgt caaacacatc ggccaccgcc acgatgcgcg cttcgatggg
     3301 gatctggtcg cccgccaatc cttccggata acccgagcca tcgaccgcct cgtggtgatg
     3361 cagggcgatg ttccgcagca tgtccacatg ggtgaccccg ttcaaaccgt aattcagcag
     3421 cagcttgtcg atcattctgc gcccttcccg ggaatgggtg cgcatgatgc tgtattcagc
     3481 gtcgtcgagc gggccttcct tcaaaagaat gcggtccgga atggccagct tgcccagatc
     3541 gtgcagcggc gcgaacagga aaacatgctc gatgtactgg tcatcgaaac caaaccggct
     3601 ggcgaggttt tttgcgatca ggcgcgcata acgcgacatc cgctccaggt ggcaaccggt
     3661 ctccgggtcg cgggagtgtg tgagatccag cgccgaccgg atggtggcga gcatggtgcg
     3721 cacattggtg cgctcgttgt agatcagcag cgcgatcatg tggccgatca catccagttc
     3781 gttgagtaca cgattggtga agacatcggt cagatgggag ttgaaaaata caaagccgaa
     3841 aaaattgcct tcccacacca tgggcaaggt atagctggaa cgataaccgc tggcgtaaat
     3901 gacgcgcgta tgttcgtgat cgacatcgtt gtagatggag agatcattca ccacgcgcgg
     3961 ctgtccgctg gtaatgatgt tgtgcaggga atggcagctg gccagcttgg cggaataatg
     4021 agtcagcggg ctggcttccc ggctgctgaa ggcgaaggtg cgcaggtcat ccgtatcggc
     4081 gtcgtacaga gcgaccgcaa gccgggtcac gaacggcatg tgtgtctgca tcacaccgtg
     4141 catgaactgc agttttcccc caatggaatt cgcatcattc aggccggtcc agcgatcgat
     4201 atgcagcggg tcatccattc tgatagtcca gatacagtag gtgcttgcca gtaactatag
     4261 caggcgccgc cggttcccaa cggttggcag aagtgaggta tattttccct acccccgtca
     4321 ttcacaagga agcgacatga agccggatcg gttgcacatt ctccagtggt tcaggtggtc
     4381 atcatgagca gcctgcgtac tgaacaagat tctctcggcc ccgtagaagt tcctgctgat
     4441 tgcctgtggg gcgcgcagac ccagcgttcc cttgagcatt tcgccatctc caccgagcgc
     4501 atgccggatg aattcattca ggccctcgcg gccgtcaagc tggcctgcac caaggtaaac
     4561 cgggatctgg gcctgctgcc ggaaagtcac gccaacgcca tcatggatgc ggccggcgaa
     4621 gtgatgatcg gccagcattt cagggaattc ccgctgtcca tctggcaaac cggttccggc
     4681 acgcagacca acatgaacat gaacgaagtg ctggccaacc gcgcgtcgga actgctgggc
     4741 ggtgaacgtg gtgaactgcg ccaggtgcat cccaatgatc aggtgaatct cgggcaatcg
     4801 tcgaatgacg tgtttcccac cgccatgcat ctggcggcag cgcggggcgt gttccttttg
     4861 cttctgcccg ccctgtccgt tctgcagaag gcgctggcgg ccaagtccgc tgcgtttgct
     4921 gacatcatca agatcggccg cactcatctg caggacgcca cgccgctgac cctggggcag
     4981 gaattttctg gctacgccgc ccagctcgac caggcccgcc tgctgatcga gcagacgttg
     5041 ccggcaattt atccgctggc gatcgggggg act
//
LOCUS       DASUEG010000926         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1250847, whole genome shotgun sequence.
ACCESSION   DASUEG010000926 DASUEG010000000
VERSION     DASUEG010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250847"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX11_17485"
     CDS             <1..258
                     /locus_tag="VFX11_17485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4940468.1"
                     /translation="GGTISSLLDLTGALAAWSGHDPSRGMKAATVSMTVNYLSAALGC
                     DIIASATAVKRGRELIFTEVAISEESSGRPIANGTMVYRIA"
     gene            complement(301..1902)
                     /locus_tag="VFX11_17490"
     CDS             complement(301..1902)
                     /locus_tag="VFX11_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4940469.1"
                     /translation="MDNRYDFIVVGAGSAGCVLANRLSESGKFSVCVVEAGPHDNSGF
                     VNVPFGVIGLIREGKRNWGYYTAPQSELGNRRLYWPRGRTLGGSSSINAMVYIRGQHL
                     DYDDWQDAGAAHWGWQQVRPVFNAHENNEQYLPDDWHGRGGPLNVTRVTDPNPLSAMF
                     VRAGVELGYPENDDFNGASQMGFGRFQVTQKDGRRWSAARAFLDPAKARPNLTIMTDM
                     LVTRVLVDNGRARGIECLDAQGKTVTLTCQREVVLSGGAINSPQLLMLSGIGDRQHLE
                     EIGIRCIVDSPQVGRNLQDHLDMTVSIRDKSRQSIGFSPFFLPRLIRAFYDYVRHKRG
                     FLASNAAEAGAFLNVGGGARPDIQLHFIPAFLRDHGRELTPGFGCTVHVCQLRPRSRG
                     YICLQDNDPRSAPLIDPRYLSDQDDIRVLREGVKLVRRLFQTDAFAPAFGGEDLPPAT
                     VKTDAQLDEDIRQRSETIYHPVGTCRMGEDRLAVVDSQLRVKGISGLRVADASVMPLL
                     ISGNTNAPCMMIGERAAGFMLEESA"
     gene            complement(1895..3004)
                     /locus_tag="VFX11_17495"
     CDS             complement(1895..3004)
                     /locus_tag="VFX11_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940470.1"
                     /translation="MDFKLSENTTMIADTAKRYLKDQYSFDIYRSVLAQTNHLESSRW
                     EMMQELGWFGLPFSEAVGGYGGDLIDVAMIVSEMGASLCLDPYVDMVVAPGKLLESVD
                     SEPARQLLASIIDGKSAVACGFYDIHSGYDALSPSLVIEGNRLSGQKSFVPDGGFAQS
                     VLVTARRGKELALVHVRLSDCEVNNHRALDGSRMSSIVFDDVMLDDDAIIACGAEAES
                     AATQTLAWLQALDCARMYGAARALYEKTLDYAKTRKQFAVHIGSFQVIQHYLVDMFID
                     LQQLESMLLMVSIKAASGDAAERDRACAAAKAYFADKAVRIAQQAIQIHGGVGVTEEL
                     DVGHYFRLITHCSLSHGAGDIQVERFAALSEAAHG"
BASE COUNT          722 a          949 c          776 g          593 t
ORIGIN      
        1 ggcggtacca tcagcagctt gctggatctg accggcgcgc tggccgcctg gtccggtcac
       61 gacccctccc gaggcatgaa agcggcgaca gtctccatga ccgtgaacta tctttccgca
      121 gctctcggct gtgacatcat cgccagcgca acagctgtca aacgtggccg cgagctgatc
      181 tttaccgagg tggcgatcag tgaggaaagc tcaggaagac cgatcgccaa cgggacaatg
      241 gtctaccgta tcgcctgaat cgcagcgcac cgggcggcag gcaacacacc gcccggcggc
      301 tcatgcgctc tcctcaagca taaagcccgc agcacgctca ccgatcatca tacaaggcgc
      361 attggtgttg ccgctgatca gcaatggcat caccgatgca tcggcaacac gcaagccact
      421 gatgcctttg actcgaagct ggctatcaac caccgccaga cggtcttcac ccatgcgaca
      481 ggtgcccact ggatggtata tcgtctctga ccgctgccga atatcctcgt ccagctgcgc
      541 atccgttttt accgttgccg gaggcagatc ttcgccacca aaggcgggcg caaaagcatc
      601 agtctggaac aggcgacgca ccagttttac cccctcacgt agcaccctga tatcgtcctg
      661 gtctgacaaa tagcgcgggt caatcaaggg cgcgctacgc gggtcattat cctgcaggca
      721 aatgtaaccc cggctccttg gccgcaactg gcaaacgtga acagtacaac cgaaacccgg
      781 tgtcagctcc cggccatgat cacgcaggaa ggcggggata aaatgcagct gaatatcagg
      841 gcgcgcacca ccgccaacat tcaaaaacgc acctgcttcg gccgcgttac tggcaaggaa
      901 gccgcgctta tgccgcacat agtcataaaa tgccctgatc agcctcggca ggaaaaaagg
      961 cgagaagccg atcgactgcc ggctcttgtc acgaatggat accgtcatat ccaggtgatc
     1021 ctggagattc ctgcccacct ggggggagtc gacgatacac ctgatgccaa tttcctcaag
     1081 gtgctggcgg tcgccgatac cggacaacat cagtaactgg ggtgagttga tggcaccgcc
     1141 tgacagcacg acctcccgct gacaggtcag tgtgacggtt ttcccctggg catcaaggca
     1201 ttcaatgccg cgcgctcggc cgttgtcgac aagcacacgg gtcaccagca tatcggtcat
     1261 gatcgtcagg ttcggccgcg ctttcgccgg atccagaaat gcgcgcgccg cactccagcg
     1321 tcgcccgtcc ttctgggtca cctggaaacg cccgaagccc atctgtgagg cgccgttgaa
     1381 gtcgtcgttc tcaggatagc caagctcgac gccggcgcga acaaacatgg cgctgagggg
     1441 attcgggtca gtgacccggg tgacattgag ggggccaccc cgaccgtgcc agtcgtcggg
     1501 caagtattgc tcattgttct catgagcatt gaataccggc ctgacctgct gccagcccca
     1561 gtgggctgct ccggcatcct gccaatcgtc ataatcgaga tgctggccac ggatatagac
     1621 catggcattg atcgagctgc ttccacccag cgtcctgcct ctcggccagt acagacgtcg
     1681 attgccgagc tcactctggg gggcagtata gtaaccccag tttcttttac cttcgcgtat
     1741 caggccgatc acgccgaacg gaacattaac gaaaccgcta ttatcatggg gcccggcctc
     1801 gaccacacaa accgaaaact ttcccgattc actcaatcga ttcgccagaa cacagcccgc
     1861 agaaccggca ccgacaacaa tgaagtcgta acggttatcc atgtgcggcc tcactcaagg
     1921 cggcaaaccg ctcgacctgg atgtcgcccg ccccatggga aagcgagcag tgtgtaatca
     1981 aacgaaaata atggccaaca tccagctcct cggtaacccc gacgccacca tgaatctgga
     2041 tggcttgctg ggcgattcgc acagccttgt cagcgaagta ggctttggcg gcagcacagg
     2101 cgcggtcgcg ctctgccgca tcgccgctgg cagccttgat tgaaaccatc agcaacattg
     2161 attccagctg ctggagatca ataaacatat cgaccagata gtgctggatt acctggaaac
     2221 tgccaatgtg gacagcaaac tgcttgcgcg tctttgcata atcaagtgtt ttttcataga
     2281 gtgctcgggc agcaccatac atacgcgcac aatcaagggc ctgcaaccag gcaagcgtct
     2341 gagtcgctgc cgattctgcc tcggcgccgc aagcaatgat cgcgtcatca tcgagcatga
     2401 catcatcgaa cacaatgctg gacattcggg agccatcaag cgccctgtga ttattcactt
     2461 cacagtctga caaacgcaca tgcaccagcg ccagctcctt ccctctgcgc gccgtcacca
     2521 gaacgctttg cgcaaacccg ccatccggga caaaactctt ctgcccggaa agcctgttac
     2581 cttcaatgac caacgatggg ctcagcgcat cataaccact atggatatca tagaatccgc
     2641 aggccactgc cgattttcca tcgatgattg aagccagcaa ttgtcttgcc ggctccgaat
     2701 caacagactc aagaagcttc cccggcgcca ccaccatatc gacgtaggga tcaagacaaa
     2761 gcgacgcgcc catttcactg acaatcatgg caacgtcgat gagatcacca ccatagccgc
     2821 cgaccgcttc agaaaatggc agaccaaacc agccaagctc ctgcatcatc tcccagcgcg
     2881 atgattcaag gtgattagtc tgagcgagaa cacttctata aatatcaaag ctgtactgat
     2941 ccttgagata gcgcttggcg gtatccgcga tcattgtcgt attttcggag agcttgaaat
     3001 ccattgcctt acctcacaga cccagcaaca tttttgccag
//
LOCUS       DASUEG010000927         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_579290, whole genome shotgun sequence.
ACCESSION   DASUEG010000927 DASUEG010000000
VERSION     DASUEG010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579290"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /gene="rapA"
                     /locus_tag="VFX11_17500"
     CDS             <1..1826
                     /gene="rapA"
                     /locus_tag="VFX11_17500"
                     /inference="COORDINATES: protein motif:HMM:NF003426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEX4940471.1"
                     /translation="RFNDIALIAEHLIDHDTLAAEHLEQLKVLLSDANSHRLITLLQA
                     DTGDKAGEALAARTLATRLLDQHGTGRALFRNTRASISGFPQRKLTRVRLPNPACYAD
                     VKINVLEDALYPEIDTPEGQWLAQDPRVNWLLETLKKRRSEKFLIICHHKDTACALQA
                     HLHFKGGIQCSAFHEDLTLIERDRSAAWFASHEDAAQALICSEIGSEGRNFQFCHNLV
                     MFDLPASIDQLEQRIGRLDRIGQKQDINIYTPYLGDTWQQHLLEWYDEGFGAFDKTES
                     SHSVVFESLRGQFLVALQQDEHALDKLVKTTRARIGDLHQRFIHGRNRLLEIHSKGDG
                     SVQELLDSVAEADNDPNLKEYMELVFSAYGVEEEDLSDTVILLRPGSSLEHNSFPSLP
                     EDGVSATYQRAVALGREDIEFLSWDHPMVRNAMDMVLTSGKGSSVLSLLKNKQVKEGT
                     VLLEALYIVECPAPAELQIQQVLPPTPVRLLLDPKGRDLSAAVDTATLDKQLKRIKND
                     LAAPMLKEIQEPVNEMLAKCEALMDTRVQQVREQALAAFDAYWENEARRLQALAQNMA
                     QPADIAPQLAAVAQKREQGRKVLNSQCTSRLDALRIMISVG"
     gene            1823..>2307
                     /locus_tag="VFX11_17505"
     CDS             1823..>2307
                     /locus_tag="VFX11_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4940472.1"
                     /translation="MIANLLPADADLVILFEDDHLFVVDKPAGLLSVPGRLPQHQDSI
                     QLRAARRFPQALVVHRLDCATSGVMVLAKTKEAQSELSRQFRDRETAKEYIAVTFGNC
                     PARTGEVDLPLIVDWPNRPLQKICFEHGKPSLTRYELLDRDGQRNRLRLIPVTGRSHQ
                     LR"
BASE COUNT          485 a          789 c          684 g          349 t
ORIGIN      
        1 agcgctttaa cgacatcgcc ctgatcgccg agcacctgat cgaccacgac accctggcag
       61 cggagcatct ggaacaactg aaagtgctgc tcagcgacgc caacagccac cgcctgatca
      121 ccctgctgca ggccgacacc ggcgacaagg ccggcgaggc gctggccgcc cgcaccctgg
      181 ccacccgcct gctggaccag cacggcaccg gccgcgcgct gttccgcaac acccgcgcca
      241 gcatcagcgg cttcccccag cgcaaactca cccgcgtgcg cctgcccaac ccggcctgct
      301 atgcggacgt gaaaatcaat gtcctggaag atgcgcttta tccggaaatc gacaccccgg
      361 aaggccagtg gctggcccag gacccccgcg tcaactggct gctggaaacg ctgaagaaac
      421 gccgcagcga aaaattcctg atcatctgcc atcacaagga caccgcctgt gccctgcagg
      481 cgcacctgca tttcaagggc ggcatccagt gcagtgcctt ccacgaagac ctgaccctga
      541 tcgaacgcga ccgcagtgcc gcctggttcg cctctcacga agatgctgcc caggcgctga
      601 tctgttccga gatcggcagc gaaggccgca acttccagtt ctgccacaac ctggtgatgt
      661 ttgacctgcc cgcctcgatc gaccagctgg agcaacgcat cggccgtctc gaccgcatcg
      721 gccagaagca ggacatcaac atctatacgc catatctggg cgacacctgg cagcagcacc
      781 tgctggaatg gtacgacgag ggcttcggcg ccttcgataa aaccgaatcc agccacagcg
      841 tagtgttcga atccctgcgc gggcagttcc tggtagcgct gcagcaggac gagcacgccc
      901 tcgacaagct ggtgaaaacc acccgtgccc gtatcggcga cctgcaccaa cgtttcatcc
      961 acggccgcaa ccggctgctg gaaatccatt ccaagggtga cggcagcgtg caggaactgc
     1021 tggattcagt ggcggaggcc gacaacgacc cgaacctgaa ggaatacatg gaactggtgt
     1081 tcagtgccta cggagtggaa gaggaagacc tgtcggacac ggtgatcctg ctgcggcccg
     1141 gttcctcgct ggaacacaac agcttcccct cgctgccgga agacggcgtc agcgcgacct
     1201 accagcgcgc ggtggccctc ggccgcgagg acatcgaatt tctgtcctgg gatcacccca
     1261 tggtgcgcaa tgcgatggat atggtgctta ccagcggcaa gggcagctcg gtgttgagcc
     1321 tgctgaagaa caaacaggta aaagaaggca ctgtgctgct ggaagccctc tacatcgtgg
     1381 aatgcccggc accggcggag ctgcagatcc agcaggtatt gccgcccacc ccggtgcgcc
     1441 tgctgctgga cccgaaaggc cgcgacctgt cagccgccgt ggacactgcc accctcgaca
     1501 agcagctcaa acgcatcaag aacgatctgg ccgcgcccat gctgaaggag atccaggaac
     1561 cggtgaacga gatgctggcc aaatgcgaag ccctgatgga tacccgggta cagcaggtgc
     1621 gcgagcaggc cctggcggca ttcgacgcct actgggaaaa cgaagcccgc cgcttgcagg
     1681 cactggccca gaacatggcg cagccggccg acatcgcccc gcaactggcg gcggtagcgc
     1741 aaaagcgcga acagggccgc aaggtgctga acagccagtg caccagccgg ctggatgccc
     1801 tgcgtatcat gatcagtgtg ggatgatcgc taacctgctc cccgcggacg ccgatctggt
     1861 catcctgttc gaggacgatc acctgttcgt ggtggacaag ccggccggac tgctgtcggt
     1921 gcccggccgt ctgccgcagc accaggattc catccagctc cgcgctgccc gccgcttccc
     1981 gcaggcgctg gtggtgcacc ggctcgactg cgccaccagc ggcgtgatgg tgctggcgaa
     2041 aaccaaagag gcgcagtccg aactcagccg ccagttccgg gaccgggaaa ccgccaagga
     2101 atacatcgcg gtgacctttg gcaactgccc ggccaggaca ggcgaagtcg acctgccact
     2161 gatcgtcgac tggcccaacc ggcccctgca gaagatctgc ttcgagcacg gcaaaccctc
     2221 cctgacccgc tatgaactgc tggaccgcga cggccagcgc aaccggctgc ggctgatccc
     2281 ggtcaccggc cgctcacacc agctgcg
//
LOCUS       DASUEG010000928         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1030044, whole genome shotgun sequence.
ACCESSION   DASUEG010000928 DASUEG010000000
VERSION     DASUEG010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030044"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFX11_17510"
     CDS             <1..440
                     /locus_tag="VFX11_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940473.1"
                     /translation="CHVQDQGNPLIDIPVVRPADTWFGPNTVRGEICYTTQVTGRLEL
                     SQLTPRPFRAVTPVQISNQGNELMPVERINLPVPFLPVYGAESGRLWTPTLHVTTEER
                     QRVPRIRIDSTISTEAGHVTLLSPARRGSDEHALIRVFDNLFD"
     gene            449..1309
                     /locus_tag="VFX11_17515"
     CDS             449..1309
                     /locus_tag="VFX11_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX4940474.1"
                     /translation="MADFKLESLFNPERLTDISAGLVLILIGWLLARLLSSAFVRAMQ
                     HRLTPHQLLLGRRTIFYLLLILFTLSGLREMGFKLSVLLGAAGVLSVAIGFASQTSAS
                     NLISGLFLIGEGPFSIGDVIRVGQTEGEVLSIDLLSVKLRTGDNLFVRIPNEQLIKSE
                     VINLTRFPIRRHNLPIGIAYKEDISKVREVLLRVARQNPLCLDEPAPTIIVQGFGASS
                     VDLMFYVWTRREQFLDMRDSMQEAIKRAFDEADIEIPFPQVSVHAGSGTDPLPLRLVR
                     DPHAGGSGDQ"
     gene            complement(1322..>2206)
                     /locus_tag="VFX11_17520"
     CDS             complement(1322..>2206)
                     /locus_tag="VFX11_17520"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4940475.1"
                     /translation="DKRDIFRGYELGAVDFLAKPIQREFLLSKVNVFLDLDRQKRDLA
                     LQLTEIQRLKQQNEMLLHALGDAVVAVDAHGVVSFANPAMQHVFHLEPSSLVGMPLND
                     LMFQNTEGVRTTWGTGKIYAATSRGERLPPTRNYYVKTDAGFIEAEVHASPVASADKE
                     FAGAVITLRAITYEETGDLSEDLARKGRRHTRKKVGAILRVFDRTTGKNLGRLLNVST
                     EGFKLAAREGLIVGHRYEVSMILPEPLAGSNTLSFDAEAVWSKPAEDAPGEYRAGFRI
                     VKITPNDARVLAQMIEKY"
BASE COUNT          468 a          702 c          569 g          467 t
ORIGIN      
        1 cttgccatgt ccaggaccag ggcaatccgc tgatcgacat cccggtcgta cgcccggctg
       61 acacctggtt cggcccgaat acggtccgtg gcgaaatctg ctataccacc caggtgacgg
      121 gcaggctgga actcagccaa ctcacgccac gtcccttccg cgccgtgacc ccggtgcaga
      181 tcagcaacca gggtaacgaa ctgatgccgg tagaacgcat caatctgccc gtaccctttc
      241 tgccggttta tggcgccgag tcaggccgcc tctggacacc caccctgcat gtgacgacgg
      301 aggaacgtca acgcgtgcct cgcatccgca tcgacagcac catcagtacc gaagcgggtc
      361 atgtcaccct gctcagtccc gcccgccgcg gctccgacga gcacgcattg atacgtgttt
      421 tcgacaacct gtttgactga gagtcaccat ggccgacttc aaactcgaat ccctgttcaa
      481 ccccgaacgg ctgaccgaca tctcggccgg attggtactc atcctgattg gttggctact
      541 ggcccgactg ctatccagcg ccttcgtccg ggccatgcag caccgcctga cccctcacca
      601 gctactgctg ggcagacgaa cgatttttta cctgctgctg atcttgttca ccctttccgg
      661 cctgcgggaa atgggattca aactgagcgt gctgctgggg gctgccggcg tactgtcggt
      721 ggccatcgga tttgcctccc agacgtctgc ctccaacctc attagcggcc tgttcctgat
      781 cggtgagggg cctttttcga taggcgatgt cattcgtgtc ggccagaccg aaggcgaagt
      841 gctgtcgatc gacctgctgt ccgtcaaact gcgcaccggt gacaacctgt tcgtccgcat
      901 accgaacgaa cagctcatca agtccgaagt gatcaatctg acgcgcttcc cgattcgccg
      961 ccacaacctg ccgatcggta tcgcttacaa ggaagacatc agcaaggttc gcgaggtatt
     1021 gctgcgagtg gcgcgccaga atccgctttg cctggacgag ccggcgccca ccatcatcgt
     1081 acagggcttt ggcgcttcat cagtggacct gatgttctac gtctggaccc ggcgcgaaca
     1141 gtttctggac atgcgcgact ccatgcagga ggccatcaag cgcgcttttg acgaggcaga
     1201 tatcgaaatt cctttcccgc aagtctccgt acatgcagga tcaggaacgg atcccctgcc
     1261 cttgcggctc gtgagggatc cacacgccgg cggttccggc gatcaatgat caacacaaga
     1321 gtcagtattt ctcgatcatc tgggcgagta cacgggcatc attcggtgtg atcttcacaa
     1381 tccggaatcc cgccctgtac tcaccgggag cgtcctcagc aggcttcgac cagacagcct
     1441 cggcgtcaaa actcagcgta ttggaccccg ccagcggctc aggcaaaatc atgctcacct
     1501 catagcggtg accaacaatc aggccttcac gagcagccag cttgaagcct tccgtggaca
     1561 cgttcagcag acgccccaga ttcttgcctg tggtccggtc aaacacacgc aatatggcac
     1621 caactttttt gcgggtatgc cgacgcccct tacgtgccag atcctcagac agatcgccgg
     1681 tttcctcata cgttatggcg cgcaacgtga tgactgcgcc ggcaaattcc ttgtcggcag
     1741 aggccaccgg cgatgcatgc acttctgcct cgatgaatcc ggcatccgtc ttcacataat
     1801 agttacgtgt aggcggcagt cgctcgccgc ggcttgtcgc ggcataaatc tttcccgttc
     1861 cccaggtcgt acggacacct tccgtgttct ggaacatcag gtcattgagt ggcatgccga
     1921 caagactgga gggctccaga tgaaaaacgt gctgcatcgc cggattggcg aaactgacaa
     1981 cgccatgagc atcaacagca acaaccgcat cacccagcgc atgaagcaac atttcgttct
     2041 gctgctttaa tcgctgaatc tctgtcaatt gcaatgcaag gtcacgtttc tgcctgtcca
     2101 gatcaagaaa tacattaacc ttcgacagca aaaactcccg ctggataggc ttcgccagga
     2161 aatccacggc accaagctca tatccacgga agatgtcacg tttgtc
//
LOCUS       DASUEG010000929         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1427499, whole genome shotgun sequence.
ACCESSION   DASUEG010000929 DASUEG010000000
VERSION     DASUEG010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427499"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFX11_17525"
     CDS             complement(<1..479)
                     /locus_tag="VFX11_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4940476.1"
                     /translation="MADVQKPKKFDRNLIVIGAGSAGLVTSYIAAMVKAKVTLIEGHR
                     MGGDCLNTGCVPSKSLLRSARMIADIRRARDFGLKPMRAEFEFAEVMARVQRSVRAIE
                     PHDSIQRYSELGVECISGYAKLVSPWEVEVNGQRLSARSIVIATGARPSVPPIPGLA"
     gene            complement(472..1158)
                     /locus_tag="VFX11_17530"
     CDS             complement(472..1158)
                     /locus_tag="VFX11_17530"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX4940477.1"
                     /translation="MKKKAVFLLVLVACVVAAWTLFDLGRYLSFYYIQSKQAEWQLAL
                     EQHPVWVGAAFFLVYVLVTGLSIPGAAVMTLLAGSLFGLALGTLIVSFASTLGATLAF
                     LLARFIGRDFVQKRFGDQLQTINRGVENEGGFYLFTLRLAPVFPFFLVNILMALTPIR
                     LWSYVWISQLGMLAGTLVYVNAGTQLGQLESMQGILSPALIASFVLLGLLPLVAKKIV
                     EFLRAKRAHG"
     gene            1263..2276
                     /gene="hemB"
                     /locus_tag="VFX11_17535"
     CDS             1263..2276
                     /gene="hemB"
                     /locus_tag="VFX11_17535"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4940478.1"
                     /translation="MPINTLNRGPFPATRMRRLRAAEFTRRLVRETLLTPADLIYPMF
                     VLEGKGVREPIASMPGIERVSLDILQEEARHLSGLGVPAIAIFPVTPQSAKSLDAQEA
                     WNPNGLAQRAIKAIKDAVPGMGVITDVALDPFTTHGQDGIIDDQGYVLNDITVEALIK
                     QAQSHADAGADIVAPSDMMDGRIGAIRRQLEYNGHANVCILAYSAKYASSYYGPFRDA
                     VGSASNLKGGNKYTYQMDPANLNEALHEVALDLQEGADMVMVKPGMPYLDIVREVKSQ
                     FGAPTFVYQVSGEYAMHVAAFQNGWLNRDAVILESLTCIKRAGADGILTYFAKSAAEL
                     LNR"
     gene            2357..>2816
                     /locus_tag="VFX11_17540"
     CDS             2357..>2816
                     /locus_tag="VFX11_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX4940479.1"
                     /translation="MTTNAPAEVTEQPAIDLHNSAYYFNRYLSLLEFNLRVLAQAEDK
                     TLPLLERLRFLMIFSSNMDEFFEIRLAGLQRDISYNQARPEADGMHPQEVLRVISGRC
                     RIAVDRQYKLFNEDLLPALAAENIHFIAREKWSAPQVQWIKHYFRQQVAPV"
BASE COUNT          611 a          940 c          794 g          471 t
ORIGIN      
        1 ttcgccagac cgggaatcgg cggcaccgac ggccgcgcgc cggtggcgat cacaatgctg
       61 cgggcggaca ggcgctggcc attcacctcc acttcccagg gcgacaccag cttggcgtag
      121 ccgctgatgc actccacccc cagctcggag taacgctgga tcgaatcgtg cggctcaatc
      181 gcacgcacac tgcgctgcac ccgcgccatc acctcggcaa attcaaattc cgcccgcatc
      241 ggtttcagac caaaatcccg ggcacggcgg atatcggcaa tcatgcgcgc gctgcgcagc
      301 aggctcttgc tgggcacgca gccggtgttg aggcagtcgc cgcccatgcg gtggccttcg
      361 atcagcgtca ctttcgcctt caccatggcc gcgatgtacg aagtgaccag cccggcggaa
      421 ccggcgccga tcacgatcag gttgcggtcg aattttttcg gcttctgaac atcagccatg
      481 ggcacgtttc gctctcagga attccacgat ttttttcgcc accagcggca gcagacccag
      541 cagcacgaac gacgcgatca gcgccggcga cagaatgccc tgcatggact ccagctgccc
      601 cagctgggtg ccggcattca catacaccaa cgtgcccgcc agcatgccca gctggctgat
      661 ccagacataa ctccacaacc ggatcggcgt cagcgccatc aggatgttca ccaggaaaaa
      721 gggaaacacc ggtgccagcc gcagggtgaa caggtagaag ccgccctcgt tttccacacc
      781 gcggttgatg gtctgcagct ggtcgccgaa acgcttctgc acgaaatcgc ggccgatgaa
      841 acgggccagc aggaacgcca gggtagcccc cagggtactg gcgaacgaca cgatcaacgt
      901 acccagcgcc agtccgaaca gggacccggc cagcagcgtc atcaccgccg caccgggaat
      961 cgacaatccc gtcaccagca catacaccag gaagaacgcc gcccccaccc acaccggatg
     1021 ctgctccagc gccagctgcc attccgcctg ctttgactgg atgtagtaga aactcaggta
     1081 gcggcccaga tcgaacaggg tccaggctgc caccacgcag gccaccagca ccagcagaaa
     1141 caccgctttt ttcttcataa atgccttgct gtcctcatcc gcgctaagac gtcactccgc
     1201 cgacggagta ttattgtgat ttaaaggtcc aacagaatcg cacgatcaca caaggtaatg
     1261 ccatgcccat caataccctg aatcgcggcc cctttcctgc cacccgcatg cgccggctgc
     1321 gcgccgcgga attcacccgc cgtctggtgc gcgaaacgct gctcaccccc gccgacctga
     1381 tctacccgat gtttgtgctg gaaggcaaag gcgtgcgcga gccgattgcg tcgatgccgg
     1441 gcatcgagcg ggtgtcgctg gacatcttac aggaagaggc gcggcacctg agtggcctgg
     1501 gtgtgccggc gattgcgatc tttccggtca caccccagtc ggccaagagc ctggatgcac
     1561 aggaagcctg gaaccccaac ggcctggccc agcgcgccat caaggccatc aaggacgccg
     1621 tgcccggcat gggcgtgatc accgacgtgg cgctggaccc attcaccacc cacggccagg
     1681 acggcatcat cgacgatcag ggttatgtgc tcaatgacat cacggtggag gccctgatca
     1741 agcaggccca gtcccacgcg gacgccggcg ccgacatcgt ggcgccctcc gacatgatgg
     1801 acggccgcat cggcgccatc cgccggcagc tggagtacaa cggtcacgca aatgtctgca
     1861 ttctggccta ttctgccaaa tatgcctcca gttactacgg cccgttccgc gacgcggtgg
     1921 gctcggcgtc caacctcaag ggcggcaaca aatacaccta ccagatggac ccggccaacc
     1981 tgaacgaggc actgcacgaa gtggcgctgg acctgcagga aggcgccgac atggtgatgg
     2041 tgaaacccgg catgccctac ctggacatcg tgcgggaagt gaagagccag ttcggcgcgc
     2101 ccaccttcgt gtatcaggtg agcggcgagt acgccatgca cgtggccgcg ttccagaacg
     2161 gctggctcaa tcgcgacgct gtcatactgg agtcattaac ctgtattaaa agggccggcg
     2221 ccgacggcat cctcacctat tttgccaaaa gcgccgccga actgctgaat cgctgatgga
     2281 ctatttattc gaacccgagc catttgaatc cgagtcattt gaacccgttg ccattcaaac
     2341 ccctgaagtg agcaccatga ccaccaacgc ccctgccgaa gtcaccgaac aaccggccat
     2401 cgacctccac aacagcgcgt actatttcaa ccgctacctg agcctgctgg aattcaacct
     2461 gcgggtactg gcccaggcgg aggacaagac cctgccactg ctggaaaggc tgcgtttcct
     2521 gatgatcttc agcagcaaca tggacgagtt tttcgaaatc cgtctggcgg gactgcagcg
     2581 cgacatttcc tacaatcagg cgcggcccga agccgacggc atgcatccgc aggaagtgct
     2641 gcgggtgatc agcgggcgct gccgcatcgc ggtagaccgc cagtacaaac tgttcaacga
     2701 agacctgctg ccggccctgg cggcggagaa catccatttc atcgcacggg aaaaatggag
     2761 cgcaccgcag gtgcagtgga tcaagcacta cttccgccag caggtggcac cggtga
//
LOCUS       DASUEG010000930         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_129652, whole genome shotgun sequence.
ACCESSION   DASUEG010000930 DASUEG010000000
VERSION     DASUEG010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129652"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX11_17545"
     CDS             <1..264
                     /locus_tag="VFX11_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940480.1"
                     /translation="ASDYMKIHAEQIRAFLPGRTYYTLGTDGFGRSDSREKLRHHFEV
                     NRYFIAIAALNALADDGEIGRSVVEDAIRRFDINPEKPYAPKA"
     gene            313..2013
                     /gene="aceF"
                     /locus_tag="VFX11_17550"
     CDS             313..2013
                     /gene="aceF"
                     /locus_tag="VFX11_17550"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX4940481.1"
                     /translation="MTEIRVPDIGSDSADVIEILVKPGDHLSPEQPIVVLESDKASME
                     VPVPQGGVVRDIAVKVGDSVKLGDLLLQLDDAVAGSSADVREIQQELQQVSSVLEPAG
                     ASPVSAPIVAPAVPAPAHVVPVAEASVTSVQLVKVPDIGSDEADVIEVLVKVGDVLRL
                     EQPIVVLESAKASMEVPSPVEGTVLNVLVKVGDKVSEHVGLLEVSVASPAAAVVEKPA
                     PVAHAASVPANAGMSHAVLSAPAVVSAPAPVPTPAMPEPTHQMVPMANVHAGPAVRRL
                     ARELGVDLGKVKGSGPKERILKDDVHAYVKSVMQAKAVPAAVEGGAGLPKLPVIDFSK
                     WGEIERLPLTKIQRVSAQNLHRAWVTIPHVTQFDEADITELEAFRVAQKDTLKAEGLN
                     LTVLAFLVKACAHVLRQFPKFNSSLDSDGENLVYKKYVHIGVAVDTPNGLVVPVIRDA
                     DKKSVREIARDMGELSAKARDKKLTPAEMQGACFSISSLGGIGGTAFTPIVNWPEVAI
                     LGVSRSATKPVWNGKDFQPRLMLPLSLSYDHRVIDGADAARFTTALSKVLADIRQLLL
                     "
BASE COUNT          401 a          549 c          709 g          430 t
ORIGIN      
        1 gccagtgact acatgaaaat ccatgccgag cagattcgcg ccttcctgcc gggacgcacc
       61 tattacacgt tgggtacgga tggttttggc cgctccgatt cgcgtgaaaa gctgcgccac
      121 catttcgagg tgaaccgtta cttcatcgcc attgcggcac tgaatgcgtt ggcagacgac
      181 ggcgagatcg gtcgcagcgt ggtcgaggat gcgattcgtc gctttgatat caatcctgaa
      241 aaaccctacg cgccaaaagc gtgaagggaa tgaacgtcgt gacgcttatc cagcgcaggc
      301 aaggagaatg aagtgaccga aatacgtgtt ccggacatcg gcagtgatag cgcggatgtc
      361 attgagattc tggtaaagcc aggtgaccac ctgtcaccag agcagccgat cgtggtgctg
      421 gagtccgaca aggccagcat ggaagttccc gtcccgcagg gcggggtggt gcgtgacatt
      481 gccgtgaagg tgggcgacag cgtcaagctg ggcgacctgt tgctgcaact ggatgatgct
      541 gtggcgggtt cgtctgccga tgtgcgtgaa atccagcagg agctgcagca ggtgagctcg
      601 gtgctggagc cggcaggtgc cagccctgtt tcggccccga tcgttgcgcc agctgtacca
      661 gctccggcac atgttgtgcc ggttgccgag gcatccgtta catcggtgca actggtgaag
      721 gtgccggaca tcggctccga cgaagccgat gtgattgaag tgctggtgaa agtgggtgat
      781 gtgctccggc tggaacagcc catcgtggtg ctggagtcgg ccaaggccag catggaagtg
      841 ccgagccctg tcgagggcac ggttctgaat gtgttggtga aggtgggcga caaggtgtcc
      901 gagcatgtgg gcttgctgga ggtgtcggtg gcatcaccgg cggcagctgt tgtagaaaag
      961 ccggcaccgg ttgctcacgc tgcttcggtg ccggcgaatg cgggcatgtc tcatgcagtg
     1021 ttatcggcac ctgcagtggt gtcggcgcca gccccggtcc cgacgccggc catgccggag
     1081 ccgacacacc agatggtgcc gatggccaat gtgcatgccg gccctgccgt gcgtcgcttg
     1141 gcgcgtgagc tgggtgttga tctgggcaag gtgaaaggca gcgggccgaa agaacgcatt
     1201 ctcaaggatg acgtgcatgc ctacgtgaag tcggtcatgc aggccaaggc ggtgccggcg
     1261 gcggtggaag gcggtgcggg cttgcccaaa ctcccggtga tcgacttcag caagtggggc
     1321 gagattgagc gcctgccgct gacgaaaatc cagcgtgtgt cggcgcagaa cctgcaccgt
     1381 gcgtgggtga ccatcccgca cgtgacgcaa ttcgacgagg ccgacatcac cgagctggaa
     1441 gcgttccgtg tggcacagaa ggatacgttg aaggcggaag ggctcaacct gaccgttttg
     1501 gcctttctcg tgaaagcctg tgctcatgtg ttgaggcagt tccctaaatt caacagctcg
     1561 ctcgacagtg atggcgaaaa cctggtgtac aagaaatatg tgcatattgg cgttgccgtg
     1621 gatacgccga atggcttggt cgtgccggtg attcgcgatg cggacaagaa atccgtgcgc
     1681 gagattgccc gtgacatggg cgaactgtcg gccaaggccc gggacaagaa gctgacaccg
     1741 gccgagatgc agggcgcctg cttcagtatt tcgtctctgg gcggcattgg tggcacggcc
     1801 ttcacgccca tcgtgaactg gccggaagtg gccattctag gggtgtcgcg ctcggcaacg
     1861 aagccggtct ggaacggcaa ggatttccag ccgcggctga tgttgccgct gtcgttgtcc
     1921 tatgaccacc gggtgatcga cggcgccgat gccgctcgct tcacgacggc cttgtcgaag
     1981 gtgttggcgg atatccggca gttgctgttg taagcactgt tttgtgcaaa taaaaagccc
     2041 gcatcgttgc gggcttttta tttgggtgcg gttcacgtct ccgggtgtc
//
LOCUS       DASUEG010000931         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1316808, whole genome shotgun sequence.
ACCESSION   DASUEG010000931 DASUEG010000000
VERSION     DASUEG010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316808"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFX11_17555"
     CDS             <1..651
                     /locus_tag="VFX11_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX4940482.1"
                     /translation="VAMRDFAATNPNAFFYQKPITLEQHQQSRMIADPLRLLDCCQES
                     DGAVAFVVTSVERAKHLANVPAVIAGARQSIVSTTRMMTPFYGESISGIPEFDACADD
                     VYGMAGLSPADIDMACLYDHFSPWVLPQLEAFGFCKRGEAKDFVKDGHIGRGGKLPVN
                     THGGQLGEAYIHGMNGIAEAVRQIRGTSVNQVADVNHVLITAGAGVPTGAAILEKG"
     gene            657..1124
                     /locus_tag="VFX11_17560"
     CDS             657..1124
                     /locus_tag="VFX11_17560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4940483.1"
                     /translation="MSKTVALETVPEGFAPNTIGPYSNLIGPLLYKRDTDEQGRPRGT
                     VGILLQDHHVGGNGRGHGGLLLTLLDEAMGMNAYLQKDQVPTVTVSMSTNFIGATLPG
                     QFLYATADIIQITSTLAFMEGKAYCGGKLVGTGSGVWKYLHSTRAQQQAQQQQ"
     gene            1137..1919
                     /locus_tag="VFX11_17565"
     CDS             1137..1919
                     /locus_tag="VFX11_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940484.1"
                     /translation="MTDFSNKSVIVTGGTKGVGFGIAQAFLQAGARVFICGRNAPAQL
                     PQGHSRQAEFIAADVRKPEESQQLIDTVLQRTGRLDVLINNAGGSPPVKAADAPSKLT
                     ESIIRLNLIAPLVFSQQAYHALRKNENGGSIINIASISGVRPSPGTAAYGAAKAGLIN
                     VTRSLAQEWAADKVRVNAIIAGMIKTEASEDHYGGEKGIALIEQSLPMGRMAVPADIA
                     NACLLLAQDSSSYISGAALDVTGAGEPPMFLQLAEQANALKQ"
BASE COUNT          471 a          658 c          622 g          377 t
ORIGIN      
        1 gtcgccatgc gcgatttcgc cgccaccaat cccaacgcat ttttttacca gaagccgatc
       61 acgctggaac agcatcagca atcccgcatg atcgccgatc ccttgcgtct gctggattgc
      121 tgccaggaaa gcgatggcgc agtggcgttc gtggtcactt cagtggaacg cgcgaaacat
      181 ctggccaacg tccctgccgt gattgccggc gcccgccaga gcatcgtcag cactacgcgc
      241 atgatgacac ccttttacgg cgaatcgatt tccggcattc ccgaattcga tgcctgcgcc
      301 gatgacgttt acggcatggc aggattgtca cccgccgaca ttgacatggc ctgcctgtac
      361 gatcattttt caccgtgggt attgccgcag ctggaagcct tcggtttctg caagcgcggc
      421 gaggcaaagg atttcgtcaa ggacggccat atcggccggg gcggaaaact gccggtgaat
      481 acccatggtg gccagctggg cgaagcgtac atccacggca tgaacggcat tgccgaggcc
      541 gtgcggcaga ttcgcggcac gtccgtaaac caggtggccg atgtcaatca tgtgctgatc
      601 accgccggcg ccggcgtgcc caccggtgca gcgattctgg aaaaaggctg aaatgcatga
      661 gtaaaacggt ggcactggaa accgtgccgg aaggctttgc gcccaacacc atcggaccct
      721 attccaatct gatcgggcca cttctgtaca agcgcgacac cgatgaacag gggcgccccc
      781 gcggtaccgt gggcattctg ctgcaggatc atcacgtggg tggcaacgga cgcggtcacg
      841 gtggcctgtt gctgaccctg ctggacgaag ccatgggcat gaacgcctac ctgcaaaaag
      901 atcaggtgcc cactgtcacc gtgtcgatga gcacgaattt catcggcgcc accttgccgg
      961 ggcaattcct ctacgccacc gccgacatca tccagatcac gtccaccctc gccttcatgg
     1021 aaggcaaggc atactgtggc ggcaaactgg taggcactgg cagcggcgtg tggaaatacc
     1081 tgcactccac ccgcgcacag cagcaggcgc aacagcaaca ataagcgaac gacaccatga
     1141 ctgatttcag caacaagagc gttatcgtca ccggcggcac caagggtgtg ggctttggca
     1201 tcgcgcaggc ttttctgcag gccggtgccc gtgtgttcat ctgcggccgc aacgcaccgg
     1261 cgcaactgcc gcaaggccac agccggcagg cggaattcat cgccgccgac gtgcgcaaac
     1321 cggaagaatc gcaacagctg atcgacaccg tactgcaacg taccggccgc ctcgacgtgc
     1381 tgatcaacaa tgccggcggc agtccacccg tcaaggccgc tgacgcgcca tcgaaactca
     1441 ccgagtccat catccgcctg aatctgattg cgccgctggt attttcccag caggcgtatc
     1501 atgcgctccg caaaaatgaa aacggcggca gcattatcaa tatcgccagc atttccggcg
     1561 tgcggccgtc acctggcaca gccgcttatg gagcagcgaa ggccgggctg atcaacgtga
     1621 cgcgctcgct ggcacaggaa tgggcggcgg acaaagtgcg cgtcaacgcc atcatcgccg
     1681 gcatgataaa aaccgaagcg tcagaagatc attacggcgg ggaaaaaggc atcgccctga
     1741 tcgaacagtc gctgccgatg ggacgcatgg cagtgccggc ggacatcgcc aacgcctgtc
     1801 tgctgctggc gcaggacagc tccagctaca tcagcggcgc ggcactggat gtcaccggcg
     1861 ccggcgagcc gccgatgttt ctgcaactgg cggaacaggc aaacgcgctg aagcaataat
     1921 ggtttgatgt tgaaatgtgt gcagaaagag gcgatcaacg cgctcttcct gcacacagcg
     1981 gcatcaaggc aagatgtaat tcgcagaaag cgccaggaag aaagtctcga ctcccattcg
     2041 tcccggacaa ccactcttca ccagctgccc aggccggcag gtcggcgtat ccaccggctg
     2101 gccgttggcg tcgtagggtt gggcaatt
//
LOCUS       DASUEG010000932         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1139794, whole genome shotgun sequence.
ACCESSION   DASUEG010000932 DASUEG010000000
VERSION     DASUEG010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139794"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFX11_17570"
     CDS             complement(<1..229)
                     /locus_tag="VFX11_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4940485.1"
                     /translation="MSLKDTLAQCRTSNDYSALVGAVPYARLLGIGCIKIGDDVVFTL
                     PALRDNIGNPTLPAIHGGVLGGFIEHSAILQV"
     gene            complement(232..675)
                     /locus_tag="VFX11_17575"
     CDS             complement(232..675)
                     /locus_tag="VFX11_17575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4940486.1"
                     /translation="MLRRYLKEVRHSMVLGMELKQVSPEGILVRLPYSDQIVGNPETG
                     VIHGGAITSLMDQACGLAVAAALAPEFDITPTIDLRIDYMRPAEPGKDVFAFVEAYRK
                     TRQVVFTRGIAFHEDRNKPIAHCVANFTRMGLNITPWGTTSPKEA"
     gene            complement(757..1470)
                     /locus_tag="VFX11_17580"
     CDS             complement(757..1470)
                     /locus_tag="VFX11_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HEX4940487.1"
                     /translation="MALHQEIMLGLSLADWLAASWFMLCWAGYTSYAERKARRRDCLS
                     SILHRQRRRWMSEMIARENRVADAALLANLERNVAFFASSTLIILAGVFTVLSATDKA
                     ISIAAELPFAVDAPAAAWEVKLILLTGIFVYAFFKFTWSLRQYGFASVVIGMAPGDGS
                     RERVNADTFAEEAAQVISRAALAFNLGLRSYYFGLAFLIWFVNPWFFALATTWVVGVL
                     YRREFKSNVLQALVRTYNA"
     gene            1544..2440
                     /locus_tag="VFX11_17585"
     CDS             1544..2440
                     /locus_tag="VFX11_17585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4940488.1"
                     /translation="MPSPAPPTRLATAVTFAILATMAGTLAAAAAKYLSNYVSTGLIV
                     FVQYAVCLLTLIPWMRKAGLSGMRTQHLWPHLIRGLSGWLCFYTYFVAVKHIPLVEAA
                     LLRNTAPLCVPFIVYFLHRSGFTLAKTIGALTGFVGVILILHPEGTVVSLWHGVGFLS
                     GVTLALSMVYTRELSADEPANRILFYYFAISTVLSAPLAAVDLKPFPLHIVPLLGFVG
                     ISIFITMKLYTIAYSAAPTNTLAPFSYFGVIFAGLLGWLFWDQMPDSRSLIGMGIVVA
                     GGVITLLARDRTAPDKAATTPG"
     gene            2557..3741
                     /locus_tag="VFX11_17590"
     CDS             2557..3741
                     /locus_tag="VFX11_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX4940489.1"
                     /translation="MDLYSRYKSAFSGLSHPFAWVDMEQLDHNIALHLKRARSMPIRI
                     ASKSIRSVAILRYLLNASAQMQGIMCYHPGEAAWLASQGFDDLLVAYPSLDTHSLNAA
                     LDQAAQGKTIYFMVDLPAHAQAISDLARQKNVTALLCLDLDMSIQYPFLYFGVHRSTV
                     RNAEDAVALYKKIRAMPNVELRGVMGYEAQIAGLGDKVPGQTLKNTAIKLLKQNAIPK
                     VESRRTSTVLALRAAGADIQLVNGGGTGSVESTIAEPSVTEVTVGSGFYCSHLFDNYE
                     ALSARPAAGFALQVTRHPRTHILTCNGGGYVASGTAEKLKLPKPYLPAGMTLDANEGA
                     GEVQTPIHFQGPDLHTGDPVFFRHAKAGELCERFNTLYLLRGTAIEERVSTYRGDGQV
                     FM"
     gene            3775..5208
                     /locus_tag="VFX11_17595"
     CDS             3775..5208
                     /locus_tag="VFX11_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX4940490.1"
                     /translation="MKRMHDYIIVGSGASGSVLAHYLTQAGADVLMLEAGKRFTAATF
                     PQNEMDANAQLMWNGGMDMSRDAATLFIRGKVLGGGTVINQCLLDRFDDVALNDWRTT
                     AGIDFFRTQDMERHYDAVESNLSLHYMQQADWNRNAELYVEGFEKLGLEWAPLRRGQS
                     DCGKGNDCIVCLGGCPRKSKQSMLETFLPKAEKNGLKILSEFEVAEVVHGQQFVTVQG
                     RHQGHPHSLYSRQVILAAGALGTTQLLLKAGVQKRLPALGRNFHCHPQWMTVGFFDEP
                     VDSHKGAFQSVKSSDPRFRDWGFKLENVFAGPIAMSLLKPGYGFEHQRFMQRYRNMAC
                     IEVAVRDQAAGTLALSNKGRLVIDKPLSDGDRQRANRGNQIVHDIFSTLGAREIMTSP
                     IQIALHLMGGCSIGTRASDSVVNEQFQVHGLPRLRIADSSIFPNAPGINPSLSIMALS
                     HRAAETLLAEAGIVASTSTTRKQEQQA"
BASE COUNT         1086 a         1583 c         1520 g         1034 t
ORIGIN      
        1 tcacctgcag gatcgccgag tgctcgatga aaccacccag cacgccgcca tggatcgccg
       61 gcagagtggg attgccgatg ttgtcccgca gcgccggaag cgtgaacacc acatcgtcgc
      121 caatcttgat gcaaccaatc cccagcaaac gggcataagg caccgcaccc accagggcgg
      181 aatagtcgtt ggaagtgcgg cactgggcca gtgtgtcctt caggctcata ttcaggcctc
      241 ctttgggctg gtggtgcccc agggcgtgat gttcaggccc atgcgggtaa aattggcgac
      301 acaatgcgcg atcggtttat tgcgatcttc atggaaggcg atgccgcggg tgaacaccac
      361 ctgccgcgtc ttgcgatagg cctcgacgaa ggcgaacaca tccttgcccg gctccgccgg
      421 ccgcatgtag tcgatgcgca gatcgatggt gggcgtgatg tcgaattccg gcgccagcgc
      481 cgccgccact gccaggccac aggcctggtc catcagactg gtgatggcgc ctccgtgaat
      541 cacaccggtt tccggattgc cgacgatctg gtccgaatag ggcaaccgca ccaggatgcc
      601 ttccggcgac acctgcttca gctccatccc cagcaccatg ctgtggcgca cctctttcag
      661 ataccggcgc agcatgcgtt ccagatcgaa gggtggtttt gcttctgtgt tctctgtttt
      721 gtcggtggac acggatgttc tcctctatgc aggcggtcag gcgttgtagg tgcgcaccag
      781 cgcctgcaat acgttggatt tgaattcccg ccggtacagc acgcccacca cccaggtggt
      841 ggccagcgcg aaaaaccagg gatttacaaa ccagatcaga aaggccagac caaaataata
      901 ggaacgcaac cccaggttga aggccagggc ggcgcgggaa atcacctggg ccgcttcctc
      961 ggcaaaagtg tcggcattca cgcgttcacg gctgccatcg cccggcgcca tgccgatcac
     1021 cactgatgca aatccatact gccgcagtga ccaggtaaac ttgaaaaagg catagacaaa
     1081 gatgccagtg agcaggataa gcttcacttc ccacgccgcg gccggggcat cgacagcaaa
     1141 cggtaactcg gcggcgatgg aaatagcctt gtcggtggcg ctgagcacgg tgaacacgcc
     1201 ggccagaatg atcagggtgg acgacgcgaa gaaagccaca ttgcgttcca gattcgccag
     1261 cagtgccgcg tcggccaccc ggttttcgcg ggcgatcatc tcgctcatcc agcgcctgcg
     1321 ctgccggtgc aggatgctcg acagacaatc ccgtcgccgc gccttgcgtt cggcataact
     1381 ggtatagccg gcccagcaca acataaacca gctcgcggcc agccagtcgg caagggaaag
     1441 acccaacatg atttcctgat gcagcgccat gatccccgat ctttgaaagc agaattttgc
     1501 aataatagca gacatttagc gcaacttgac caggaacaga cccgtgccct cgcccgctcc
     1561 tcccacccgg cttgccaccg ccgtcacctt tgccattctt gcgaccatgg ccgggacgct
     1621 ggcggcagcg gcggcgaaat acctgtcgaa ctacgtcagc accgggctga tcgtattcgt
     1681 gcaatacgcg gtgtgcctgc tgacgctgat tccctggatg cgcaaggccg gtctgtcggg
     1741 catgagaaca cagcacctgt ggccgcacct cattcgcggc ctgtcgggct ggctgtgctt
     1801 ttacacctat tttgtcgcgg taaaacacat tccgctggtg gaagcggcgc tgctgcgcaa
     1861 taccgcgccc ctgtgcgtac ccttcattgt ctatttcctg catcgcagtg gatttaccct
     1921 cgccaaaacc atcggcgcac tgacgggctt tgtcggcgtg atcctgatcc tgcatccgga
     1981 gggaacagtg gtgagccttt ggcacggcgt gggttttctg tcgggggtga cactggcact
     2041 gtcgatggta tacacgcggg aactgtcggc agacgaaccg gccaatcgca ttctgttcta
     2101 ctacttcgcg atatccaccg tactgagcgc gccactggcg gcggtggacc tgaaaccgtt
     2161 cccgctgcat atcgtgcccc tgctgggctt cgtcggcatt tccatcttca tcaccatgaa
     2221 gctctacacc atcgcctact cggccgcgcc caccaacacg ctggcgccgt tttcctattt
     2281 cggtgtgatc ttcgcaggtc tgctgggctg gctgttctgg gaccagatgc cggacagccg
     2341 cagcctgatc ggcatgggaa tcgtggtggc gggtggcgtg atcaccctgt tggcgcggga
     2401 caggacggca ccggacaaag cggccacgac tccggggtaa tccggccaga ccgacgtttg
     2461 tggttgtgac ccctcaccaa attgtagtac catcgttcca atatttgctg ccacacgcct
     2521 caccataaca ataagctggt tcgagccacc gccattatgg atctgtattc acgttacaaa
     2581 tctgcatttt ctggcctgtc gcaccccttt gcctgggtgg acatggagca actggaccac
     2641 aacatcgcgc tccatctgaa acgcgcgcgc agcatgccga tccggattgc gtcgaaatcg
     2701 atccgcagtg tggccatcct gcgctacctg ctcaatgcca gtgcgcagat gcagggcatc
     2761 atgtgttatc accccgggga agccgcctgg ctggccagcc agggattcga cgatttgctg
     2821 gtggcctacc cgagcctgga cacacacagc ctgaacgcgg cactggatca ggctgcgcaa
     2881 ggcaaaacaa tttatttcat ggtggatttg cctgctcatg ctcaggcaat ttccgatctg
     2941 gcgcggcaaa aaaatgtcac cgccctgctc tgcctggatc tcgatatgtc gatccagtac
     3001 ccgtttctgt attttggcgt gcatcgctcc accgtgcgca atgccgagga tgccgtggcc
     3061 ctgtataaaa aaatccgcgc catgccgaat gtggaactgc gtggcgtaat gggatacgaa
     3121 gcacaaatcg ccggcctggg cgacaaggtg ccgggccaga cactgaaaaa caccgcaatc
     3181 aagctattga agcagaatgc gattccgaaa gtcgaatcgc gccgcaccag cacggtactc
     3241 gcattgcgcg cggcaggcgc cgacattcag ctggtcaacg gcggcggcac tggcagtgtg
     3301 gaatccacca ttgccgaacc ctcggttacc gaggtcaccg tgggcagcgg cttctattgt
     3361 tcccatttgt tcgacaatta cgaagcgctc agtgcgcgac cggccgcagg atttgcactt
     3421 caggtcacgc ggcatccccg gactcacatt ctgacctgca atggcggcgg ttatgtggcg
     3481 tcgggcacgg cggaaaagct gaaactgccc aagccatacc tgcccgccgg catgacgctg
     3541 gatgccaacg aaggtgccgg cgaagtgcag acacccattc atttccaggg accggacctt
     3601 cacaccggcg atccggtttt tttccgtcat gcgaaagcag gcgaattgtg cgagcgtttc
     3661 aatacgctct atttgttgcg gggcacagca atcgaggaac gggtcagcac ttaccgcggt
     3721 gatggccagg tgttcatgta aacgatgttc aggtaaacag ggatttttat tgcaatgaaa
     3781 cgaatgcacg actacatcat cgtcggctcc ggtgcttccg gcagcgtgct ggcgcactat
     3841 ctgacgcagg ccggcgccga cgtgctgatg ctggaggccg gcaagcgttt cactgccgcc
     3901 accttcccgc aaaacgaaat ggatgccaac gcccagctga tgtggaacgg cggcatggac
     3961 atgagccgcg acgccgccac gctgtttatc cggggcaagg tgttgggcgg cggcacggtc
     4021 atcaaccagt gtctgctgga tcgtttcgac gatgtggcgc ttaacgactg gcgtaccacc
     4081 gccggcatcg acttcttccg cacgcaagac atggaacgcc actatgacgc agtggaatcc
     4141 aacctgtcgc tgcattacat gcagcaggcc gactggaacc gcaatgcgga actgtatgtg
     4201 gaaggttttg aaaagctggg gctggaatgg gcaccgctgc gccgcggcca gagcgactgc
     4261 ggcaagggta acgactgcat cgtctgcctg ggcggctgcc cgcgcaaatc caagcaaagc
     4321 atgctggaaa cctttctgcc gaaagcggaa aaaaatggcc tgaaaatcct gtcggaattt
     4381 gaagtggcgg aagtggtgca cggccagcag tttgtcaccg tacaggggcg acaccagggg
     4441 catccgcatt cgctgtacag ccgtcaggtg attctggctg ccggcgcgct gggcaccaca
     4501 cagttgttat tgaaagccgg tgtccaaaaa cgcctgccag cactgggccg gaatttccac
     4561 tgccatcccc agtggatgac agttggtttc ttcgacgagc cggtggattc ccacaagggc
     4621 gcattccagt cggtgaaatc gtccgatccg cgcttccgcg actggggttt caaactggaa
     4681 aacgtgttcg ccggccccat cgccatgagc ctgctgaaac cgggctatgg ctttgagcac
     4741 cagcggttca tgcagcgtta ccgcaacatg gcctgcattg aagtggcggt gcgggatcag
     4801 gcggcgggca cccttgcact gagcaacaag gggcggcttg tgatcgacaa gccgctgtct
     4861 gacggagacc gtcagcgcgc caatcgcggc aaccagatcg tgcatgatat tttcagtacg
     4921 ctgggcgcac gtgaaatcat gacctcgcca atccagattg cgctgcacct gatgggcggc
     4981 tgcagcattg gcacccgcgc cagcgattcc gtggtgaacg aacagttcca ggtccacggc
     5041 ctgccacgtc tgcgcattgc ggattcgtcg attttcccca acgcgcccgg catcaatccg
     5101 tcactgtcga tcatggcgct gtcgcaccgg gcggcggaaa ccttgttggc ggaagcaggc
     5161 attgtggcca gcacctccac cacgcgcaaa caggagcaac aggcatgaat ccgggcgcat
     5221 cca
//
LOCUS       DASUEG010000933         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_587733, whole genome shotgun sequence.
ACCESSION   DASUEG010000933 DASUEG010000000
VERSION     DASUEG010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587733"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VFX11_17600"
     CDS             complement(<1..1329)
                     /locus_tag="VFX11_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX4940491.1"
                     /translation="MDILFIFGLILLNGVFAMSEIALVSSRQVRLEKEAADGSESAKA
                     ALKLSREPTRFMSTIQVGITLIGILAGAMGEGSIAERIEVYLLEVDILARWAEPLSLT
                     LMVIIITYFSLIFGELVPKRLAMHNPEAIAKFVAHPMNLLSKFARPLVLLLSISTEAI
                     LKLLGTKQAEEMDIIEEEIHSLMKQGTEAGVLEESEHTMVRNVLRLDDQRVSNIMTLR
                     KEVYYIDLEDGSEENLLKIKDSTHSRIPVCKGGIDNVIGFLYSKDVMNQVLSGKSLDI
                     SDMIKRPLFVPNSATALQLLEQFKKSRVHLAIVVDEHGQTSGLVSVNDVLEAIVGDMP
                     ADDQEYEPDFIEREDGSWLVNALVDISEFKEKFGIRSLPEEEQGNYSTLGGLIMTVLG
                     HIPKVTTVLTLANVQLEVLDMDGNRVDKVLVTRLLTPLPDNDNPPEVQSGY"
     gene            1492..>2021
                     /locus_tag="VFX11_17605"
     CDS             1492..>2021
                     /locus_tag="VFX11_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017219318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4940492.1"
                     /translation="MPTLFTRRPLALAMACLASAPVLAQETDTTADATLEPVEVWATE
                     VKASSVKLDGEAMAIKQADHISDLLRPVPGVDVGGAHSLNQRITIRSMDDKDIRVSID
                     GANQNTYMYHHMGNLQINADILKSVEIDVGNNSVVNGGLGGAVRFETKQAKELLRPGQ
                     DFGARVQASYGDNAYVG"
BASE COUNT          383 a          559 c          639 g          440 t
ORIGIN      
        1 ataccccgac tgcacttccg gcgggttgtc gttgtccggc aggggcgtga gcaggcgtgt
       61 gaccagcacc ttgtccaccc ggttgccgtc catgtccagc acctcaagtt gcacgttggc
      121 cagggtgagc acggtggtga ctttgggaat gtggcccagc acggtcatga tcaggccgcc
      181 cagggtggag tagttaccct gttcttcctc cggcagtgac cggatgccga atttttcctt
      241 gaactcgctg atgtccacca gcgcattcac cagccagctg ccgtcctcgc gctcgatgaa
      301 atccggttcg tattcctggt cgtccgccgg catgtcgccc acgatggctt ccagtacgtc
      361 gttcactgat accagaccac tggtctggcc gtgttcgtcc accacgatgg ccaggtgcac
      421 gcgggatttc ttgaactgct ccagcagttg cagcgcggtg gcggagttgg gcacgaacag
      481 cgggcgcttg atcatgtccg agatatccag cgatttgccg ctcagtacct gattcatcac
      541 gtccttggaa tagaggaagc cgatcacgtt gtcgatgccg cccttgcaca cgggaatgcg
      601 cgagtgggtg gaatccttga tcttcagcag attctcttcg ctgccgtctt ccaggtcgat
      661 gtaatagacc tccttgcgca gcgtcatgat gttgctgacg cgctggtcgt ccagccgcag
      721 cacgttgcgc accatggtgt gttcggattc ctccagtacg ccggcctcgg tgccctgctt
      781 catcagcgag tggatttcct cttcgatgat gtccatttcc tcggcctgct tggtgcccag
      841 cagcttgagg atggcctcgg tggaaatgct cagcagcagc accagcggtc gcgcgaactt
      901 ggacagcagg ttcatggggt gggcgacgaa tttggcgatg gcctcggggt tgtgcatggc
      961 cagccgcttg ggtaccagtt cgccgaaaat cagcgagaag taggtgatga tgatgaccat
     1021 cagggtcagt gacagcggtt cggcccagcg cgccaggata tccacttcca gcaggtagac
     1081 ctcgatgcgc tcggcgatgg agccttcgcc catggcgccg gccaggatgc cgatcagggt
     1141 gatgccgacc tggatggtgg acatgaagcg ggtgggttcg cgggaaagtt tcagggcggc
     1201 tttggcggat tcgctgccgt cggcggcctc cttttccagt ctgacctgac gggaggagac
     1261 cagggcgatt tccgacatgg caaacacgcc attcaggagg atcagcccga agatgaagag
     1321 aatgtccatt aacctttgtc atgctgttga ggaggggcag aagataaagg aaatgggacg
     1381 atattggtat tggtttttga tgacacaggc agtggatggc gcattcttgt tgcaaatgag
     1441 aaatattatt aatagtattt gcgctgcttt ggccatcccc tggagttgtc tatgcccacc
     1501 ctgtttaccc gtcggccact ggcgcttgcc atggcctgtc tggcgtctgc ccccgtgctg
     1561 gcccaggaaa ctgacaccac tgctgatgcc accctggagc cggtggaggt ttgggccacc
     1621 gaagtgaagg cgtcatcggt gaagctggat ggtgaggcca tggccatcaa gcaggccgac
     1681 catatcagcg acctgctgcg gccggttccg ggggtggacg tggggggggc ccattcgctg
     1741 aaccagcgca tcaccatccg cagcatggac gacaaggata tccgcgtcag catcgacggc
     1801 gccaatcaga acacctacat gtaccaccac atgggcaacc tgcagatcaa cgccgacatc
     1861 ctgaaatcgg tggaaatcga cgtgggcaac aattcggtgg tgaatggcgg tctgggtggt
     1921 gcggtgcgct ttgaaaccaa acaggcgaag gagctgctgc ggccgggtca ggatttcggg
     1981 gcgcgggtgc aggcgtccta cggtgacaat gcctatgtgg g
//
LOCUS       DASUEG010000934         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_676295, whole genome shotgun sequence.
ACCESSION   DASUEG010000934 DASUEG010000000
VERSION     DASUEG010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676295"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..503)
                     /locus_tag="VFX11_17610"
     CDS             complement(189..503)
                     /locus_tag="VFX11_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX4940493.1"
                     /translation="MKFIFEVKMKPGFTVEEYAEGWVRASEIIQQTPGARGTYLHRKI
                     GHHDTVLAIAHWESKAHRDAKDDNRSAIVKAILEKHAQFCDITVIGEFEEPEWIVLPP
                     PT"
     gene            744..2027
                     /locus_tag="VFX11_17615"
     CDS             744..2027
                     /locus_tag="VFX11_17615"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX4940494.1"
                     /translation="MAATLLPFHADRDRLFNELHTRPFPVLEQGARVSQVALLHEGNN
                     AEAEYRHIHTLCERYSILPPASGSSCYYQNFGGFELRWERHTEFSTYTFIHRADGPDP
                     FQRTALSLLPPEWLAGLPGKVISGLHVEIQAMPEPAPVGDDLRHHFEGHRLISSWVID
                     RKARLWTAYRIHNDGLGRVLVLNKGLNPCQLGRLVRRLLELETYRMMVLLAFPMARHI
                     APGIAEMDQQLANINEQIKAIQGLEDERRLLGQLSTLAARIEHLRSDTNFRFSAARAY
                     YELVLSRLEELREQEEPGMQTFNEFLPRRLTPAFRTCEAVSGQLDDLSRRIDRASELL
                     RTRVDLTLEAQNQDLLQSMNRRSQMQLQLQQAVEGLSVVAISYYLVGLVKYMAGSLHE
                     MGVLHYPELVVGLSVPVCAVLVWAGVRRLRKALTE"
     gene            2319..>2576
                     /locus_tag="VFX11_17620"
     CDS             2319..>2576
                     /locus_tag="VFX11_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940495.1"
                     /translation="MLLATPAGKASREPELRALLAAGFFGLRSLPGDIEAGFRQTSRT
                     KAAQTLRHSIYGLIVLYLLVVVPVSLFSEDQNLSLWQLYGMF"
BASE COUNT          454 a          777 c          769 g          576 t
ORIGIN      
        1 tcgaaagggt tcctgcctgg caagctggag ggcgaagtga atcaggtgcc ggccgaattc
       61 ctgatcgacg agaatggcat catccgtgtg gcgttctacg gtaacgacat tggcgatcac
      121 ctggatatcg aggtgatcga gcgcttcctg tccccagcca tggcctgagg cagcatgtgc
      181 tgctcccgtc aggtgggcgg gggtagcacg atccattccg gctcctcgaa ttcgccgatc
      241 acggtgatgt cgcagaactg cgcatgcttt tccagaatgg ctttcacgat ggcgctgcga
      301 ttgtcatcct tggcgtcgcg atgggctttt gactcccaat gtgcaatggc cagcaccgtg
      361 tcgtggtggc cgatcttgcg gtgcaggtat gtaccgcgcg cacccggtgt ctgctggatg
      421 atttcgctgg ctcgcaccca gccctcggca tattcctcta ccgtgaagcc cggtttcatc
      481 tttacctcaa agatgaactt catgacgtca tcctcctgcg cgtggtggat tcagtttagg
      541 cagcaaatgg cgggttggcg gttgccggaa tgcacggacc gcacagtgga atatgcatcg
      601 gtgatatgac cgcgaggttt cccgtgcttt caattcccca tgctagcatg caccgttttt
      661 gctcagctcc ggagatcctc gtgacggtat acgttccccc ggccccgccg gccgcagccc
      721 ctgatatgcc ggcatccggc atcatggccg cgaccctgct gccattccat gccgaccgtg
      781 atcgcctgtt caacgagcta cacacacgtc cgtttccggt gttggagcag ggggctcgtg
      841 tctcgcaggt ggccctgctg cacgagggca ataatgccga agccgagtac cggcacatcc
      901 acaccttgtg cgagcgctat tccatcctgc cgccagcatc cggctcctcg tgctattacc
      961 agaacttcgg cggattcgag ttgcgctggg agcgtcatac cgagttctcc acttacacct
     1021 tcatccatcg cgcagacggg cccgacccgt tccagcgtac ggcgctgtca ttgctgcctc
     1081 ccgagtggtt ggcgggcctg cccggcaagg tgatcagcgg cctgcatgta gaaattcagg
     1141 ccatgccaga gccggcaccg gtgggcgatg acttgcgcca ccattttgaa gggcatcgcc
     1201 tcatcagttc ctgggtgatc gatcgcaagg cccgtctgtg gacggcctat cgcatccaca
     1261 acgatggcct gggtcgtgtg ctggtactga acaaggggct caatccctgc cagctgggcc
     1321 gcctggtgcg tcgcctgctg gagctggaaa cctaccgcat gatggtgttg ctggcatttc
     1381 ccatggcgcg tcacattgcc cccggcattg ccgagatgga ccagcaactg gccaacatca
     1441 atgagcagat caaggccatt caggggctgg aagacgagcg ccgcctgctc gggcagcttt
     1501 ccacactggc ggcccgtatc gagcatctgc gttccgatac caatttccgc ttttccgcgg
     1561 cgcgtgcgta ctacgaactg gtgttgagcc ggctggagga gttgcgcgag caggaagagc
     1621 ccggcatgca gaccttcaac gagttcctcc cgcgtcgcct gacacctgca ttccgtacct
     1681 gtgaggctgt gtcggggcag cttgatgacc tgtcacgtcg catcgaccgc gccagcgagc
     1741 tgctgcgtac ccgtgtcgat cttacgctgg aagctcagaa ccaggatctg ctgcagtcca
     1801 tgaatcgtcg cagccagatg cagttgcagt tgcagcaggc ggtggaaggg ctgtcggtgg
     1861 tggcgatcag ctactacctg gtcgggttgg tgaagtacat ggctggttcg ttgcacgaaa
     1921 tgggcgtact gcattacccc gagctggtgg tggggctttc cgtgccggta tgcgccgtgc
     1981 tggtatgggc cggggtcagg cgattgcgca aagcacttac tgaatagcgt ccatgcgcca
     2041 gctgaccgcc gtgggtcttt tgctggcggc gcgatgatgc acttcagcgt tgcctgctca
     2101 ttcattgggc aggcatgcac tcaagcatag accacacatc gacagcatga gccggtggct
     2161 caccattccc gccggtatgt gctgtcgttg gtttagcatc gggctcatgt cgtcggattt
     2221 gtcatgccga cggggccggt cgccctagac tgaaatcaag ctcaggtctc gaccatcgcc
     2281 agccaaccac aatgccaatt catatgagcg agaattccat gctgttggca acccctgcag
     2341 gcaaagcctc cagagaaccc gaactgcgtg ccctgctggc ggccggattt ttcgggctgc
     2401 gctccctgcc cggtgatatt gaagccggct ttcgccagac ctcgcgcacg aaggctgcac
     2461 aaacccttcg gcattccatt tacggcctga tcgtccttta cctgctggtg gtggtgcctg
     2521 tctcgttgtt tagcgaagac cagaacctgt cgctctggca gctctatggc atgttc
//
LOCUS       DASUEG010000935         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1228644, whole genome shotgun sequence.
ACCESSION   DASUEG010000935 DASUEG010000000
VERSION     DASUEG010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228644"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFX11_17625"
     CDS             <1..437
                     /locus_tag="VFX11_17625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4940496.1"
                     /translation="QLEARVILAGFLDNPYPVMSRARFAVFSSLAEGFPNAMAEAMAL
                     SLPVVATDCESGPAEILDDRIRLNAPTAVKAEFGILVPVNDQAAMTQALQWMGDTALQ
                     DHYRQRSRTRIRQFSVDHAVTQYGDALKLPHNIVAHGLRKPL"
     gene            470..1591
                     /locus_tag="VFX11_17630"
     CDS             470..1591
                     /locus_tag="VFX11_17630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018982686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4940497.1"
                     /translation="MKICAIGLRGIPSIMGGIEKHCEQIYPRIAALDDSIQLQVTGRS
                     PYLPTRRSNHLGVEIVALWAVRNKYLETILHTFLAILYARFVARADLVHIHAIGPGLL
                     APLARLLGLKVLITHHGADYHRQKWNSFARFLLRLGERLAISASHRTIIVGSSLCEQL
                     KRQHPRHAHKLIYIPNGASIERRPPESLRNHPVLQQFGLQPGGYVLAVGRLVPEKGFQ
                     DLVQAFRGTPLPGKLVITGGADHPDEFSRNLERQQNEQIIFTGFQSGDALAALYANAS
                     LFVLPSYHEGLPIAALEALSFGRPVLLSDIAPNQDLQLSPACYFPTGDWRALARKIQT
                     PDYSIFQTNTVDILSRFDWDEIARQTLQQMRLALGIPGE"
     gene            2227..>2697
                     /locus_tag="VFX11_17635"
     CDS             2227..>2697
                     /locus_tag="VFX11_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940498.1"
                     /translation="MPLLENRITQVDSFLERRLDDWQMTPPTWRPAGPAVASSTAVRR
                     LLFVINSLEGGGAERVFSLLVNHIQPCLHRVDIDVLLLDDKRQRYEITAPVNLFCLQS
                     DGSLGQSALRFKRFLDMRRPDLVISFLTRANYLAAAFSRFYGYRCIISERSDTTG"
BASE COUNT          574 a          827 c          748 g          548 t
ORIGIN      
        1 tgcaactgga agcacgcgtg atactggccg gcttcctcga caatccctac ccggtcatga
       61 gcagggcacg cttcgccgtg ttctcgtcac tggcagaagg ttttccaaat gcgatggcgg
      121 aagccatggc attgtcgctg cccgtcgttg cgacagattg cgaatccggt ccggcggaaa
      181 tcctggacga caggattcgc ctcaacgcac cgactgccgt caaggcagaa ttcggcatcc
      241 tggtgcccgt caacgatcag gccgccatga cgcaggcact gcaatggatg ggagacaccg
      301 ccctgcagga ccattatcgc cagcgcagcc ggacacgcat ccgccaattc agtgtcgacc
      361 acgcagtgac acagtatggc gatgcgctga aactcccgca caatattgtt gcgcacggac
      421 tgcgcaaacc actttagact catcggcaac acaggaaaat ccaggcacca tgaaaatttg
      481 cgcaatcgga ttgcgaggca taccgtccat catgggcggt attgaaaagc actgcgaaca
      541 gatttatccg cgcatcgcgg cactggacga cagcatccaa ctccaggtca ctggccgcag
      601 cccttatctt cccacccgca gatcaaacca tctcggtgtg gaaatcgtgg ccctgtgggc
      661 ggtgcgcaac aaatatctgg aaaccatcct gcacacgttt ctcgcgattc tgtatgcgcg
      721 cttcgtcgca cgggccgatc tggttcacat ccatgccatt ggccccggat tattggcgcc
      781 actggctcgc ctgctgggcc tgaaggtgct gatcacgcac cacggcgccg actaccatcg
      841 ccagaaatgg aacagcttcg cccgttttct gctgcggctg ggggagcggc tggccatcag
      901 cgcctcgcac cggaccatca tcgtgggttc gtcgttgtgt gaacaactca aacgccagca
      961 cccgcggcac gcacacaagc tgatatacat tcccaacggt gccagcatcg agcggcgccc
     1021 gcccgaatcc ttgcgcaatc atccggtgct gcagcagttt ggcctgcaac cgggaggcta
     1081 cgtgctggcc gttggacgcc tggtgccgga gaaaggtttt caggatctgg tgcaggcgtt
     1141 ccgtggcaca ccactgcctg gcaagctcgt gattaccggt ggcgccgatc acccggacga
     1201 attttcccgc aatctggagc gtcagcagaa tgaacagatc atcttcaccg gctttcaaag
     1261 cggcgatgcg ctggcagcgc tatacgccaa cgcatcgctg ttcgtgctgc cgtcctatca
     1321 tgagggcctg ccgatcgccg cactggaagc gctgagcttt ggccggcctg tcctgctgag
     1381 cgacattgca cccaaccagg acctgcagct gtcgccggcc tgctactttc ccaccggcga
     1441 ctggcgcgca ttggcgcgaa agatccagac accggattac agcatcttcc aaaccaacac
     1501 tgtcgacatt ctgtcgcgct tcgactggga tgaaatcgcg cgccagacgc tgcagcagat
     1561 gcgactggct ctgggcatac cgggtgaatg agcggggacc catgatgcct agctgaacgc
     1621 gagagcaacg gttatccaag tcggataagg cgtgcagacg catcgaggaa cgcccattgc
     1681 tactcgccgc catgaattat tgaggtgaca cgcaaaggcg caatccagcg ataaatgtag
     1741 ggatatggcc ttgcactcac atccgcgcac gcattctgca agcctttgca atggctgcat
     1801 tttcaaaatt ggaatccggt cgcggaatca gaacacgaag tccggtatta aaaacaccga
     1861 agagagagac ggcgtgaagg aatggcgacc gtggtgggta ttgcaatcct gcgttggcac
     1921 attgcggtct tttgtgtcac cattcgcaca ctccattcat gttccaggtt cgcattcgca
     1981 cgaaccggtg tcggcgagac atgaacggtt accgacatcg ccgatgcgtg tgaccctcgg
     2041 gcaccacgcc aacaagaaac tctcataatc agaattgcca gcaccattgt tggctgcgca
     2101 cttgcaccac ctgcaacgct ttgcgaggac gagaaagttt gccggattcg gcagcgggac
     2161 aggcacgccc tcctgctgtg ttatccgctc agattcgcat gcaaaccgta tggaaaagga
     2221 accaccatgc ctctgcttga aaaccgcatc acgcaagtgg attcgtttct ggaacgacgc
     2281 cttgacgatt ggcagatgac tccgccgacg tggcggccgg ctggccctgc cgttgcctcg
     2341 tccactgctg tccgacgcct gctgttcgtc atcaattcgc tggaaggcgg cggtgccgag
     2401 cgtgtcttca gcctgctggt caaccatatt cagccctgtc tgcaccgggt ggacatcgat
     2461 gtgctgctgc tggatgacaa acgccagcgc tacgaaatca ccgcaccggt gaatctgttt
     2521 tgccttcagt ccgacggcag cctggggcag agtgcgctgc gcttcaagcg tttcctcgac
     2581 atgcgccgtc ccgatctcgt gatcagcttt cttacgcggg ccaattatct ggcagcggcc
     2641 ttttcgcgtt tctatgggta ccgctgcatc atcagcgaac gcagtgatac caccggt
//
LOCUS       DASUEG010000936         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_911907, whole genome shotgun sequence.
ACCESSION   DASUEG010000936 DASUEG010000000
VERSION     DASUEG010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911907"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFX11_17640"
     CDS             <1..448
                     /locus_tag="VFX11_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4940499.1"
                     /translation="ALRMLLTYSVADSELAAFSEYASLDGARLDRVQGDTISLDLDYD
                     LAALDTTLHWDVLAVDDVDADVDLDGATLDTAKDGYVVHNISARWMPRSVDGLELTLG
                     VDNLFDEYYASQSSRTGVSLHPRFGELYLMDYEPGRNAKVTVAYQF"
     gene            448..1590
                     /locus_tag="VFX11_17645"
     CDS             448..1590
                     /locus_tag="VFX11_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4940500.1"
                     /translation="MNGCRLLGACLLLLLSIPLQAEIRITDIAGREVVLQQPAQRFVL
                     SEGRYVLTLALVRPGNPVQGLVGMMQTIGWTYPDLEEQVFRLYPEAKNIALFGVRDES
                     SVSVEKIIDLKPEVAIFGVQDHGPGAQNAELLRQLEAAGIKVVFIDFRMDPLQNTTRS
                     IAILGQLFGTETRAQSFIDLYAGKRALIEKRLADVKQRPSVFLQVHPGRFDCCMAMAD
                     GMLGPFVGLAGGRNIVDALAPGPTSEHTAEFLLMENPDVWIGTASGTAMDFKADKNIV
                     ALGAGMTAETARKSLNRFLAAPEFQALDAVKKGRAHALWHDFYNSPLNIAALEAMAQW
                     LHPALFADVDPQRTLMEIDTQFLPFDLNGTFFIGNGSSAVETGKWR"
     gene            1587..>2340
                     /locus_tag="VFX11_17650"
     CDS             1587..>2340
                     /locus_tag="VFX11_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX4940501.1"
                     /translation="MTGFGANYRALWWQRVLILGGMAVVLVMSLLLDLQAGPADLRFE
                     QMLQGLIDPASLDLRYRIILFDVRLPDALIALSVGAALGLAGIETQTILNNPLASPFT
                     LGISAFAAFGASIAIVFVPATALVSHTVVLPLLALVFALLCGMLILLFSHWTGGARES
                     VILFGMALFFLGNALTSALQYVGSAEAVQQIVFWTVGNLTKAGWTEFYIVTACLLLVF
                     PFSMRHVWVMTLIRAGEAQAVSLGLNLPRLRMI"
BASE COUNT          401 a          604 c          804 g          531 t
ORIGIN      
        1 cgcactgcgc atgctgttga cctattccgt ggcggacagc gaactggcgg ccttcagcga
       61 atatgcgtcg ctggatggcg cgcggctgga ccgtgtgcag ggtgacacca tcagcctgga
      121 tctggattac gatctggcag cactggatac gacgctgcac tgggatgtac tggcggtgga
      181 tgacgtggac gcggatgtgg atctggatgg cgccacgctg gatacggcga aggatggtta
      241 tgtggtgcat aacatttcag cccgctggat gccgcgcagt gtggatggcc tggaattgac
      301 cctgggcgtg gacaacctgt tcgacgaata ctacgcgtcg caatcctcgc gcaccggcgt
      361 gtcgttgcat ccccgtttcg gcgaactgta tctgatggac tacgagccgg gccgcaatgc
      421 caaggttacc gtggcgtacc agttctgatg aacggctgcc gtttgctggg cgcttgcctg
      481 ctgttgctgt tgtccattcc gcttcaggct gaaatccgta ttaccgacat tgccgggcgc
      541 gaagtggtgc tgcaacagcc ggcgcagcgt ttcgtgctga gcgagggccg ctatgtgctg
      601 acgctggcgc tggtgcggcc gggcaatccg gtgcaggggc tggtgggcat gatgcagacc
      661 atcggctgga cgtatcccga tctggaggag caggtattcc gcctgtatcc ggaagcgaaa
      721 aatatcgctc tgtttggtgt ccgtgacgag agcagtgtat cggtggagaa aatcatcgac
      781 ctgaaaccgg aggtggcgat tttcggcgtg caggatcacg ggcccggcgc gcagaatgcc
      841 gaattgctgc ggcagctgga agcggcaggc atcaaggtgg tgtttatcga cttccgcatg
      901 gacccgttgc agaacacgac tcgctcgatt gccattctgg ggcagctgtt tggcacggaa
      961 acgcgggcgc agtcttttat tgatctgtat gcggggaaac gtgcgctgat tgaaaagcgt
     1021 ctggcggatg tgaagcaaag gccctctgtt ttcctgcagg tgcatccggg ccgtttcgat
     1081 tgctgcatgg cgatggcgga cggcatgctg ggcccctttg tcggcctggc cggcgggcgc
     1141 aatattgtgg atgcgctggc gccggggccg acgtcagaac atacggcgga atttctgctc
     1201 atggagaatc ccgatgtgtg gattggcacg gcgtcgggca cggcgatgga tttcaaagcg
     1261 gacaaaaata ttgtggcgct gggggccggg atgaccgcag agactgcgcg caaaagtctg
     1321 aaccggtttc tcgcggcacc ggaatttcag gcgctggatg cggtgaagaa aggccgcgcc
     1381 catgcgttgt ggcacgactt ctacaactcg ccattgaata ttgccgcgct ggaagccatg
     1441 gcgcagtggc ttcacccggc gctgtttgcc gatgttgatc cgcagcggac actgatggaa
     1501 atcgatacgc agtttctgcc attcgatctg aacggtacgt tttttatcgg caacggttcg
     1561 tcggcagtgg aaaccggtaa atggagatga cggggttcgg cgcgaactac cgtgcactct
     1621 ggtggcagcg ggtgctgatt ctgggcggca tggcggtggt gctggtgatg agcctgctgc
     1681 tggacctgca ggccgggccg gcggatctga ggtttgaaca gatgctgcag ggtctgattg
     1741 atccggccag tctggatctg cgctaccgca tcattctgtt tgatgtgcgg ctgccggatg
     1801 cgttgattgc gctgtcggtg ggcgcagcgc tggggctggc aggaatcgaa acgcagacga
     1861 ttctgaacaa tcccctggcc agcccgttca cgctgggcat ttcggcgttc gctgcgtttg
     1921 gcgccagtat cgcgattgtg tttgtgccgg ccacggcgtt ggtcagtcac acggtggtgt
     1981 tgccgctgct ggcgctggtg ttcgcactgc tgtgcggcat gctgattctg ctgttttcgc
     2041 actggaccgg cggagcgcgc gagagcgtga tcctattcgg catggcgctg ttctttctcg
     2101 gcaacgcgct gacgtcggca ttgcagtatg tgggcagcgc cgaagccgtg cagcagatcg
     2161 tgttctggac cgtgggcaat ctcaccaagg ccggctggac cgagttttat atcgttaccg
     2221 cctgcctgct gctggtattt ccgttcagca tgcgccatgt gtgggtgatg acgttgatac
     2281 gcgccgggga ggcacaggcc gtcagcctgg gattgaattt gccgcgtctg cgcatgattg
//
LOCUS       DASUEG010000937         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2134416, whole genome shotgun sequence.
ACCESSION   DASUEG010000937 DASUEG010000000
VERSION     DASUEG010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134416"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX11_17655"
     CDS             <1..189
                     /locus_tag="VFX11_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX4940502.1"
                     /translation="VLESALRSTMFSMPSSSGLRRCVLQLRQDADNAALEVVTVTGEA
                     SEATPLSPAVPESLSLEM"
     gene            431..973
                     /locus_tag="VFX11_17660"
     CDS             431..973
                     /locus_tag="VFX11_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039800902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX4940503.1"
                     /translation="MARIGIFFGSNTGKTRKVSKMIKKRFDDDTMADPVNVNRTSAAD
                     VAQYQYLIFGTPTLGDGLLPGLSADCESESWEEFLPTLADVDFTGKTVAIFGLGDQVG
                     YAHEFVDAMLELYNFLKERGATIVGAWPTDGYEFDHSEAVVDGKFVGLALDLDNQSAL
                     TEQRLDAWLKSIAPAFDLNL"
     gene            complement(1066..>2811)
                     /locus_tag="VFX11_17665"
     CDS             complement(1066..>2811)
                     /locus_tag="VFX11_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4940504.1"
                     /translation="ATGQDANTNGVDDGCEDNVDGDQNGYLNDCHGINTLVSRVNANG
                     SLNPVAGDPIDGNVGHGTNMAGVMTAASGNLDNPFLGGVVGVAGIEPNIRIATCKSGQ
                     LESDVFPLIPGVAIPVASEAAIRQCMQYFHDLKMAGVNIAVVNASGGMSRFINISNVM
                     FALVNQKYWLDTPEMQTLANLMEQDDIVVVAAAGNNGWNIDQNVVERAYFPAAFTNSN
                     IISVGAINNQGELWSGSSYGRWTVDIMAPGQSILSTSPRYPLVDAQHADFVVSDGTSQ
                     ATAYVTGVVALLRANASTAGLSASAIRRLLLSSGKPLPAAASKTVGGNLVRLADANGR
                     GALSCNNQVFRRRQLPAANAVVALPGETVKIEVQNYNCAYVGTETSLPVTVTPGNTSF
                     QLRDDGVGDDTVAGDGIYSGSWTVPAGEFEYFLATGADSVTGSTDVLNIKAGIIVDNT
                     DSGADYVGKWWASIYRPGFYGSNYRYATEAEPEKLFTWSPLINEPGYYRAYARWPQGP
                     NFATNSKYRVHHQSPQDGTELITEVSMNQTINGNQWRELGLYWFNGGIQSIDLTNVNA
                     NGTVIADAIMLVPQL"
BASE COUNT          553 a          799 c          856 g          603 t
ORIGIN      
        1 gtgctggaat ccgcgttgcg cagcaccatg ttcagcatgc cgtcctccag tggattgcgc
       61 cgctgcgtgc ttcaactgcg tcaggacgca gacaatgcgg cgctcgaagt ggttacagtg
      121 accggcgagg caagcgaagc cacgccattg tccccagcag tgcccgagtc gctcagcctg
      181 gaaatgtgac gcgggttggg gctgtcgcgg atgcgacagg cccggccaac gcggccggga
      241 gcgattgtcc ggttcacgac acggctctcg gagcaacatt gcagaatgtt aatctggatc
      301 aaaaatatca atgatttgtg agtttttcag gcggaataaa gctggcgcgt tttgtgctgc
      361 tttctgactg agtgcccgga cgtcagggca gggccgcttt tcacacacca gcagatggca
      421 gaggtatgtc atggccagaa taggcatttt ctttggcagc aacaccggca agacccgcaa
      481 ggtgtcgaag atgatcaaga agcgcttcga cgacgatacg atggccgacc cggtcaacgt
      541 caaccgtacc agcgctgccg acgttgcgca gtaccagtat cttatttttg gcacacccac
      601 actgggcgat ggtctgctgc ctggtctgtc cgcggattgc gaaagcgaaa gctgggagga
      661 atttcttccc acactggccg atgtggattt tactggcaag accgtggcga ttttcgggct
      721 gggtgatcag gtgggctatg cccacgagtt cgttgacgcc atgctcgaac tgtacaactt
      781 tctgaaagag cggggtgcca ccatcgttgg tgcctggccc accgacggtt acgaattcga
      841 tcattccgaa gcagtggtgg atggcaaatt cgtcggcctg gcgctggatc tggacaacca
      901 gagcgcgctc accgaacagc ggctggatgc ctggctgaaa agcatcgcgc cggcgtttga
      961 tctgaacctg taacggcagc gtgccgctgg aatgaaaagg ccggcgcggg agttggtccg
     1021 cgccggcctt ttcacagact gccattgtcc ttgcgttgtg tcagttcaga gttgcggtac
     1081 cagcatgatg gcgtcggcga tcaccgtgcc attggcattc acgttggtca gatcgatgga
     1141 ttgaatgccg ccattgaacc agtacagccc cagctcacgc cattgattgc cgttgatggt
     1201 ctgattcatg ctgacttccg tgatcagttc tgtgccgtct tgcggcgact gatgatgcac
     1261 acggtatttt gaattggtgg caaagttcgg accttgcggc caacgtgcat aggcacggta
     1321 gtagccgggt tcgttgatca gtggcgacca ggtgaacagc ttttcaggtt cagcttcggt
     1381 ggcatagcgg tagttgctgc catagaagcc cggccggtag atcgaagccc accatttgcc
     1441 aacatagtct gcgccggaat cggtgttgtc gacaatgatg cccgccttga tattgagaac
     1501 atccgtggag ccagtcacgc tgtccgcacc ggtggcaagg aagtactcga attcgccagc
     1561 gggtacggtc cagctgccgg aataaatacc gtcgccggcc acggtatcgt cgccaacgcc
     1621 atcatcacgc agctggaagc tggtattgcc cggcgttacg gttacaggca gagaggtttc
     1681 cgtgccgacg tacgcgcaat tgtagttctg cacttcgatt ttcacggttt cgcccggcag
     1741 tgccaccacg gcattggcag caggcaattg ccggcgacgg aacacctggt tgttgcagct
     1801 gagcgcaccg cggccattgg cgtccgccag gcgcaccaga ttgccgccca ccgtcttgct
     1861 ggcggcggcg ggcaggggct tgccactgct cagcagcagc cggcgaatgg cactggcact
     1921 caggcctgcc gtgctggcat tggcccgcag cagcgccacc acaccggtga catacgcggt
     1981 ggcctgggac gtaccgtcgc ttaccacgaa atccgcatgc tgcgcatcta ccagcggata
     2041 gcgcggactg gtgctcagaa tggactgacc cggcgccatg atatccaccg tccagcggcc
     2101 atagctggag ccgctccaca gttccccctg attattaatg gcgcccactg aaatgatatt
     2161 gctgttggtg aacgcggcgg ggaaataggc gcgctccact acattctgat cgatgttcca
     2221 gccgttgttg ccggcagcgg cgaccacgac gatgtcatcc tgctccatca agttcgccag
     2281 cgtctgcatt tccggcgtat ccagccagta tttctggttg accagcgcaa acatgacgtt
     2341 ggagatgttg atgaagcgcg acatgccgcc ggatgcattc actacggcaa tattcacgcc
     2401 ggccattttc agatcgtgga agtactgcat gcactggcgg atagcggctt cgctggctac
     2461 cggaatggcg acgccgggaa tcagcgggaa aacatcggat tccagctggc cggatttaca
     2521 ggtggcgatg cggatattcg gctcgatacc ggcaacaccc accacaccgc ccaggaacgg
     2581 attgtcgagg ttgccgctgg cggcggtcat cacgcctgcc atgttggtgc catgccccac
     2641 gttgccatcg atgggatcac cggctaccgg attcagtgag ccgttggcgt tgactcgact
     2701 gaccagcgtg ttgatgccat ggcagtcgtt gagatagccg ttctggtcgc cgtcaacgtt
     2761 gtcttcgcag ccgtcatcga cgccgttggt gttggcatcc tggccggtgg c
//
LOCUS       DASUEG010000938         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_367343, whole genome shotgun sequence.
ACCESSION   DASUEG010000938 DASUEG010000000
VERSION     DASUEG010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367343"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFX11_17670"
     CDS             complement(<1..432)
                     /locus_tag="VFX11_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940505.1"
                     /translation="MNISLFLQMAADAEPDRIALVCSSRRWSYSELWQAAQGAATNIA
                     QSGCQFVALLDESSEATPIALFGAALAGVPYVPLNYRLADADLAALLSRIAPAYAIGD
                     VERIQRLNPDGHHACFKRADFVSAAEAVAEPFVEPEHEDGGI"
     gene            complement(443..733)
                     /locus_tag="VFX11_17675"
     CDS             complement(443..733)
                     /locus_tag="VFX11_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940506.1"
                     /translation="MKPGTKLKSAVCDTEVMVIKGTNIAVDCGGKPMSDTAPAAKADL
                     DAAFAEGTKMGKRYVNVDNTVELLCVKAGKGSLSIGGTPLQIKDSKPLPSSD"
     gene            complement(803..2029)
                     /locus_tag="VFX11_17680"
     CDS             complement(803..2029)
                     /locus_tag="VFX11_17680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4940507.1"
                     /translation="MTQVMKGVKVLEVAQFTFVPAAGAILADWGADVVKIEHPVRGDT
                     QRGFINMGGMKIDPQRNTMMEHANRGKRSVGIDISNPEGVELIYEIAKTADVFLTNYL
                     PFQRQKLKIDLEHIRAVNPKIIYARGSAYGDKGPERDVGGFDGTAFWSRSGVGFSMSP
                     AELGQPLPQGIAAFGDSIGGMNIAGGIAAALFHRAQTGEASEVDVSLLSTAWWAAGSA
                     VDLFIEKREVMRNRMPRSGGNPGNPFTGNYSTSDGGTINLCILTPGAYIRDTFTHLGI
                     PEAADDSRFATVESLMRNWSDASDLMAKAFASKPFEYWRQHLKTMKGQWAPVQSLLDL
                     ASDDQALANDMLFEVESVDGGDPIKLVRGPVQFNHEPVKNTRAPQASEHTETYLMELG
                     LEWERIEQLKSSGAIA"
     gene            2250..3029
                     /locus_tag="VFX11_17685"
     CDS             2250..3029
                     /locus_tag="VFX11_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940508.1"
                     /translation="MNIDSTLAAVVTGGASGLGLATVKALRSQGVKVAIFDLNKDIGE
                     KVAEETGAVFCQCDVLSDESVDNAFAQAREVNGQERVLVCCAGGGNAIPTARRDKKTG
                     EIKIFPTDQFEWVLRLNTVGTFRCITRAAAGMMAGEPVDEERGVIVNTASAAATDGQM
                     GQAAYSAAKAAIVGMTLTIARDLSRENIRVNTIQPGIFDTPAMARAPQEMKDALGAMV
                     PYPRRLGHPDEFASLVLEMIRNRYINGETVRLDGAIRMQPR"
     gene            3106..3939
                     /locus_tag="VFX11_17690"
     CDS             3106..3939
                     /locus_tag="VFX11_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4940509.1"
                     /translation="MTNEATVSSPVTYATEGAVAIISLNRPQQANAQNKELLIELDKA
                     WDRAAEDDQVKVIVLRANGKHFSSGHDLSNSNAAAQFEQDKAESGMMKLYMHERKHFL
                     GFSRKWRDIPKPSIAVVQGSCIAAGMMLCWPCDLILAADNAVFRDPVVMMGIGGVEYH
                     GHTWEFGPRKAKEMLFTASSITAEEARELGMVNRVIPLAELDSQAMELAQQIARMPAF
                     GLAMAKRAVNQTLDIMGQYAATQSVFDIHSLGHGNAVAQCGFPVLVDLEKLKQAGKKP
                     Q"
     gene            3991..4704
                     /locus_tag="VFX11_17695"
     CDS             3991..4704
                     /locus_tag="VFX11_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940510.1"
                     /translation="MMSTFSDKVVVITGAAGGLGQSIAGYFRRAGAELALIDFSEELL
                     QKVFPESDEGNEILLACDLTNKESTSDAFAKIIKRFGRIDVLANIAGGFVMGEAVHET
                     SDATWNFMFNLNTRSIVNTAAATVPVMLKQGGGKIINVSSASSQKGVGQMGAYIASKS
                     AVSRLTESMADELRGKNINVNCIMPSIIDTPANRKSMPGADYSKWVKPDDLASVVGFL
                     ASDASIAVHGAGIPVVGLV"
BASE COUNT         1019 a         1240 c         1388 g         1076 t
ORIGIN      
        1 gatgccaccg tcttcgtgct ctggctcgac aaagggttca gctacggctt cggcagcgga
       61 aacgaaatcg gcccgcttga aacacgcgtg atggccgtcg ggattgagcc gctgaatgcg
      121 ttccacatca ccgattgcgt acgccggtgc aatccgactc agcaaagccg cgaggtctgc
      181 atcggcaagc cggtaattca gtggcacata gggtacaccg gccagcgctg cgccaaacag
      241 ggcgatgggc gtagcctcgc tgctctcatc aagcagagcc acaaattggc agccgctctg
      301 tgcgatgttg gtggcggctc cctgtgcagc ttgccaaagt tcgctgtagc tccagcgccg
      361 actgctacag accagcgcaa tacgatccgg ctcggcgtcc gccgccattt gcagaaacag
      421 agaaatattc atgggcaggc cttcagtcgg atgatggtaa aggtttgctg tccttgatct
      481 gcagtggcgt gccgccaatg gacagtgagc ctttgccggc tttgacgcag agcagctcaa
      541 ccgtattgtc aacattgaca tagcgcttgc ccattttggt gccttcagca aacgctgcat
      601 ccagatcggc ctttgccgcc ggggcggtat cggacatcgg cttgccgccg cagtccaccg
      661 caatattggt tcccttgata accatcactt ctgtatcgca aactgcgctt ttcagttttg
      721 ttccaggctt cataatgctc tcgttatcgt tgctcttttt agcgttgatc ttgttcttgc
      781 gctgatcttt ttatggcgtc tttcaggcga ttgcaccgct cgatttcagc tgctcaatgc
      841 gttcccattc caggcccaat tccatgaggt aggtttcggt atgctccgaa gcctgcggcg
      901 cgcgggtatt ttttacgggt tcatgattga attgaaccgg gccgcgcacc agtttgattg
      961 gatcgccgcc atcgacggat tccacttcga acagcatgtc gttggccagg gcctgatcgt
     1021 cgctggcgag gtccagcaga ctttggacgg gagcccattg gcctttcatg gttttgagat
     1081 gctggcgcca gtattcgaat ggcttgctgg caaaggcttt cgccatgaga tcgctggcat
     1141 cgctccagtt gcgcatcagg gattccaccg tggcgaaccg gctgtcatcc gctgcttcgg
     1201 ggatgcccag gtgcgtaaag gtgtcgcgga tataggcgcc gggcgtaaga atgcacagat
     1261 tgatggtgcc gccatcggag gttgaataat tgccggtgaa ggggttgccc ggattgccgc
     1321 ctgagcgggg catgcgattg cgcatgactt cccgtttttc gataaacaga tcgactgccg
     1381 agcccgccgc ccaccaggcc gtgctgagca gcgacacatc cacttcggat gcttcgccgg
     1441 tttgggcccg atgaaacagc gctgccgcaa tgccaccggc gatattcatg ccgccaatgg
     1501 agtcgccaaa cgccgcgatg ccctgtggca ggggctggcc cagttcagcg ggggacatgg
     1561 agaagccaac gccgctgcgg gaccagaatg cggtgccatc gaagccgccg acatcgcgct
     1621 ccggcccctt gtcgccatag gcgctgccgc gtgcataaat gatcttggga ttcaccgcac
     1681 ggatatgttc aagatcgatt ttcagtttct gccgttgaaa gggcagatag ttggtaagaa
     1741 acacatcggc cgttttggcg atttcataga tcagctcgac accttccgga ttggagatgt
     1801 cgatgccaac gctgcgtttg ccgcgattgg cgtgttccat catggtgttg cgttgcgggt
     1861 cgattttcat cccgcccata ttgatgaatc cgcgttgtgt atcgccacga acagggtgtt
     1921 caatcttgac tacatccgcg ccccagtcag ccagaatggc gccagccgca ggtacaaagg
     1981 taaattgcgc tacctcaagg actttcacgc ctttcattac ttgggtcata ggttttcccc
     2041 gttcctctgc aggtgcacgt ctagcgatga agacgttgga tttggaagaa ttattcaagg
     2101 tataatacta aacaacaaca gtgttgtata taatgagttg ccgcagcaga actgccccat
     2161 atgatttgct gcttatggct gtaacccatt gaagtggtta tagatattca agcctcacat
     2221 cacaagaaat ttccagagga ttttgtgcca tgaacattga ttccactctt gccgcggtgg
     2281 tgacgggcgg tgcgtccggg ctgggtctgg cgacggttaa agccttgagg tcgcaggggg
     2341 ttaaggttgc aatattcgac ctgaacaagg acatcggcga gaaggtggcg gaggaaaccg
     2401 gcgccgtttt ttgtcagtgc gatgtgctgt ctgacgaaag cgtcgacaac gcctttgcac
     2461 aggcccgcga agtcaatggt caggaacgcg tgctggtctg ctgcgccggt ggtggcaacg
     2521 ccattcccac cgcccgtcgt gacaagaaaa ccggtgagat caagatattt ccaactgatc
     2581 agtttgaatg ggtgctgcga ctgaatactg ttggtacttt ccgttgtatc acgcgcgcag
     2641 cagcgggcat gatggcgggt gaaccggtcg atgaagagcg cggtgtcatt gtgaataccg
     2701 catcggcagc ggctaccgac ggtcagatgg ggcaagcagc ctattctgca gcgaaggccg
     2761 ctattgtggg catgacgctc accattgcgc gtgatctgtc gcgcgagaat attcgcgtca
     2821 acaccattca gcccgggatt ttcgatacgc cggccatggc cagggcgccg caggaaatga
     2881 aggatgcact gggtgccatg gtgccttatc cgcgtcgact gggccatcca gacgagtttg
     2941 ccagtctggt gctggagatg attcgcaacc gttacatcaa cggtgaaacg gttcgtctgg
     3001 atggcgccat ccggatgcag ccacgctgat ttcccacgct tcccaactcg ctgcttacgt
     3061 acaaacataa atacaaatac agccagatca gagaggatta tggtcatgac aaatgaagcc
     3121 actgtttcca gccccgtcac ctacgcaacc gaaggcgcgg tggcgataat cagcctgaac
     3181 cgtccccagc aggccaatgc gcagaacaag gaactgctga ttgaactcga caaggcctgg
     3241 gaccgcgccg ccgaagacga tcaggtcaag gtgattgtgc tgcgcgccaa cggcaagcat
     3301 ttttcatcgg gacacgatct gtcgaattcc aacgccgcag cccagtttga gcaggacaag
     3361 gctgaatccg gcatgatgaa gctgtacatg cacgaacgta aacactttct gggtttcagc
     3421 cgcaagtggc gcgatattcc caaaccgtcg attgctgttg tacagggttc ctgtattgcg
     3481 gcgggcatga tgctgtgctg gccttgcgat cttattctgg cggcggacaa tgcggtgttc
     3541 cgcgatccgg tagtgatgat gggtatcggt ggcgttgagt accacggcca cacctgggaa
     3601 ttcggtccgc gcaaggccaa ggagatgctg tttactgcgt cttccatcac ggctgaagaa
     3661 gcgcgcgaat tgggcatggt gaatcgtgtg atcccgctgg cggagctgga ttcccaggcg
     3721 atggaacttg cccagcagat cgccaggatg ccggcgttcg gactcgccat ggcaaagcgc
     3781 gcggtcaacc agacgctgga tatcatgggt cagtatgcgg caacccagtc ggtgttcgat
     3841 atccactcgc tggggcacgg caacgcggtg gcccagtgcg gattcccggt attggtagat
     3901 ctggaaaaac tgaaacaggc tggcaaaaag ccgcaatgat gtggtcgcca aaagccgacc
     3961 agcgtaacaa gccgaataag ggattgagca atgatgagta cattttccga taaagtggta
     4021 gttatcaccg gtgcggccgg agggcttggg caaagcattg caggttattt ccgtagggcg
     4081 ggcgctgaac tggcgttgat cgatttcagt gaagagctgt tgcaaaaagt atttcctgaa
     4141 agcgatgaag gaaatgaaat tcttctggct tgcgacctta ccaacaagga atccaccagc
     4201 gatgcgttcg ccaaaatcat caagcggttt ggtcgcattg atgttctggc gaatatcgcc
     4261 ggtggttttg tcatgggcga agccgtgcat gagacttcgg atgccacctg gaacttcatg
     4321 ttcaatctca atacccgttc catcgtcaac acggcggcag caacggttcc tgtcatgctg
     4381 aagcagggtg gtggcaagat catcaatgtg tcgtcagcct cttcgcaaaa aggggtaggg
     4441 caaatggggg cgtatatcgc atccaagagt gccgtttcgc gcttgacgga gagcatggcg
     4501 gatgagttgc gcggcaagaa catcaatgtg aactgcatca tgccatccat cattgacacg
     4561 ccggcgaatc gcaaaagcat gcccggcgcg gattacagca aatgggttaa accagacgat
     4621 ctggcgagtg tggttgggtt cctggcgtcg gatgcaagca ttgcggtgca cggggcaggc
     4681 attcccgtcg tcggactggt ttaatgtgac ttatttggcg tga
//
LOCUS       DASUEG010000939         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_433346, whole genome shotgun sequence.
ACCESSION   DASUEG010000939 DASUEG010000000
VERSION     DASUEG010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433346"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1349)
                     /locus_tag="VFX11_17700"
     CDS             complement(189..1349)
                     /locus_tag="VFX11_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940511.1"
                     /translation="MNLEFTDEEIAFRNMVRAWMKENVSDDLRSVTARGDMTSKDQQQ
                     RWEKQLGDKGWLALTWPEQYGGPGWTATQRYIFDLERAMAGAPPTSPFGVSMVGPVIY
                     TFGSDVQKQRWLPGIVKGNTLWCQGYSEPNAGSDLASLKTRAERKDDHYLVNGQKIWT
                     TQAHWADMIFCLVRTDSAVKQQEGISFLLIDMKTPGIEVRPIYSIDGHHHLNEVFFSD
                     VQVPLENLVGTEGMGWTIAKFLLTHERTTIAGVADSHHAMARLKEALHAAPRFAVPAQ
                     ARRRIAELEIELMALEYTNFRTVAATDEGRPFGPESSGLKIKGTELQQKLSQTMVELG
                     GLMSLVWDSEEPVGSDQFNNSPRRYNFLRASTIYGGSNEIQKNVLAKMLLGL"
     gene            complement(1391..>2506)
                     /locus_tag="VFX11_17705"
     CDS             complement(1391..>2506)
                     /locus_tag="VFX11_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940512.1"
                     /translation="ESAPMEDQAEGGQQLAGLFYTGGTTGRSKGVMLSHDNLVFNALN
                     VVPEMGYGQSTIYMHAGPMFHLADMASTFAVTLAAGTHAIVPRFDVEEVLSLIQTERV
                     THTLLVPTMINMLVSSGKIRNFDTSSLQRMLYGASPMPEAVLIEAMKQMPNAAFAQGY
                     GQTEASPILTALAPEFHVSGGAKLRSAGRAALGVEVVIMDKDDRELPCGEVGEICARG
                     ENVMLGYWGMEQASAETLRNGWLHTGDLGYMDEDGFVFIVDRAKDMVISGGENIFSVE
                     VEGAIYSHPAVQECAVVGIPHKEWGEMVHAIVVLRDGHRLDEEELQAHCRALIASYKV
                     PRSIEFREQALPVSGAGKILKNELRAPYWEKMERAVN"
BASE COUNT          576 a          767 c          605 g          558 t
ORIGIN      
        1 acttcggaaa atggcagacc gaaccagcca agctcctgca tcatctgcca gcgcgatgac
       61 tcaaggtgat tactccgggc aataacattc ctgtaaatat cgaaactgta ctgatccttg
      121 agatagcgct tggcggtatc cgcgatcatt atcgtatttt cggataaatt gaaatccatt
      181 gtcttacctc acagacccag cagcattttt gctagaacat ttttctggat ctcgttcgat
      241 ccgccgtaga tggtcgacgc gcgcaggaaa ttataacgac gcggtgaatt gttgaactga
      301 tcactgccaa ccggctcctc actgtcccat acgagagaca tcaaaccacc caactcaacc
      361 atggtctgag acagcttctg ctgcaactcg gtgcccttga tcttgagccc ggatgactcc
      421 gggccaaacg ggcgtccctc gtccgttgcg gcaacggttc ggaaattggt gtattccagc
      481 gccatcagct cgatttccag ctcggcaata cgacgacgag cctgagctgg cacggcgaaa
      541 cgcggcgccg catgcagagc ctccttcagc ctggccatgg catgatggct gtcggcgacg
      601 ccggcaatgg tggtgcgttc atgggtgaga agaaacttgg caatcgtcca gcccatccct
      661 tctgtaccca ccagattctc cagtggcacc tgaacatcgg aaaaaaacac ctcattgagg
      721 tgatgatgac catcaatgct gtaaatgggt cggacttcga tacccggcgt tttcatgtca
      781 atcaagagaa acgaaatgcc ctcctgctgc ttgacggcac tgtccgtacg caccagacaa
      841 aaaatcatgt ccgcccagtg ggcctgcgtg gtccatattt tctggccatt caccagataa
      901 tgatcatcct tgcgctcggc gcgggttttc agggaagcaa gatcggaacc ggcattgggt
      961 tccgaatagc cctgacacca caaggtattg cccttgacga tgcccggtaa ccaacgctgc
     1021 ttttgcacat cagagccaaa agtgtaaatc accgggccga ccatcgacac gccaaaagga
     1081 ctggtcggtg gcgcgccagc catggctcgt tcaagatcaa agatatagcg ctgtgtcgcg
     1141 gtccagcctg gcccaccata ctgctcgggc caggtcagag cgagccagcc tttgtcgccc
     1201 agctgttttt cccaacgctg ctgctgatcc ttgctggtca tgtccccgcg cgcagtcacg
     1261 ctgcgcaaat cgtcagagac attctccttc atccatgcac gcaccatgtt gcggaacgcg
     1321 atttcttcgt cagtaaactc aagattcacg caattcacct ttatccggtc agttatttga
     1381 aacccgccta tcagttgacg gcacgctcca tcttctccca atacggagct cgcaactcgt
     1441 tcttgaggat tttcccggcc ccactcactg gcagggcttg ctcgcggaac tcaatgctgc
     1501 gtggcacttt gtagctggca atcagcgccc ggcaatgggc ctgcagctcc tcttcgtcca
     1561 ggcggtgacc atcgcgaagg acaacgatgg catgcaccat ttctccccac tctttgtgag
     1621 gaatgccaac caccgcacat tcctgaacgg caggatggct gtagatcgcg ccttcgacct
     1681 cgacagaaaa gatattttca ccgccactga tgaccatatc tttggcccgg tcgacgataa
     1741 agacaaagcc atcctcgtcc atataaccga gatcgccggt atggagccaa ccgttacgca
     1801 aggtctctgc cgatgcctgc tccatccccc agtaacccag catgacgttc tctccgcggg
     1861 cacagatctc accaacctca ccacaaggaa gctcgcgatc atccttatcc atgatcacaa
     1921 cctcgacacc cagtgcagca cggccagcac tgcgcaattt cgcaccaccc gacacatgga
     1981 actctggcgc tagggcggtc aatatcggcg atgcttccgt ctgcccgtaa ccctgcgcga
     2041 atgctgcatt cggcatctgc ttcatggcct cgatcagcac cgcctccggc attggtgagg
     2101 cgccatacaa catgcgttgc agactactgg tatcaaaatt cctgatcttg cccgacgaga
     2161 ccagcatgtt aatcatcgtc ggcaccagca gcgtatgagt aacacgctct gtttgtatca
     2221 gtgaaagcac ttcctcgaca tcaaaacgtg gcacaatggc atgggttccc gctgccaagg
     2281 tgacggcaaa ggtgctggcc atatccgcca gatgaaacat cggcccggcg tgcatataga
     2341 tggtgctctg tccgtagccc atttcaggaa ctacattcag tgcattgaaa accagattgt
     2401 catgactgag cattacaccc ttggagcgcc cggtggttcc ccctgtgtaa aacagcccgg
     2461 caagctgttg ccctccctcg gcctgatctt ccatcggtgc agattc
//
LOCUS       DASUEG010000940         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1989101, whole genome shotgun sequence.
ACCESSION   DASUEG010000940 DASUEG010000000
VERSION     DASUEG010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989101"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFX11_17710"
     CDS             <1..242
                     /locus_tag="VFX11_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4940513.1"
                     /translation="VDQERRTALLRSVVEATPGWSRAQQEIVTASLDIFWQPSTYERL
                     INGWNFNSEQSLATLTWLIDLIETAVKDERRPEIQ"
     gene            complement(284..1615)
                     /locus_tag="VFX11_17715"
     CDS             complement(284..1615)
                     /locus_tag="VFX11_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4940514.1"
                     /translation="MEMNDMVIISVDDHITEPPDMFDKHLSADDLKTAPKFNTDKDGK
                     SYWTYQGMYMPSVGLNAVVGRPLEEYGMEPNSLEEMRKGVYNVHARINDMNVNGVAAS
                     LNFGTCVGFDGGRFHKAADKKLALKHLRAYNDWHIDEWCGAYPGRFIPCGLLPTWDMD
                     ATVAEIKRIAAKGCTAVSINENPTKQELPSVHNAYWNPMWKAIAEHDITLCLHIGAGN
                     PVPHASMETPIEAWITTMPLSSAVGAADWLHLTALHEYNLKIALSEASIGWVPYFMER
                     ADFAHSRHKFWTHNHFNGMLPSEVFKKHFMLCFIDDPYGLKNLDELNEDLIAYECDYP
                     HSDTLWPECPEHLWKTIKHLKDEQINKITHLNAMKWFNFDPFKHVKKQDLTVGALRLQ
                     AAKDKVDTTPTSYGGAAPLAAGEVPRRITSGDLMKMFAHHAQEGTKKVQAG"
     gene            complement(1742..2452)
                     /locus_tag="VFX11_17720"
     CDS             complement(1742..2452)
                     /locus_tag="VFX11_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940515.1"
                     /translation="MTQQHTGFWRRFRLTPSPNAVQAEVEDDFHCMSVVVHHDGKKAL
                     RIVPDMRRAPWSTCPGAADGLVQTFTGVALALFPERGEKKANCTHLYDLALLAASHAA
                     DRAQTIYDVLVSDPVDNQRLAEVRRNDETLLAWIESGFQIVKPDAAAGIRLDKLRSWI
                     ETLEPALQEPARLLQWGNMLANGRIIPLEKQSDATRMPPSCYTFQPDRAVVAKRIGEI
                     RDFSHGAGRPLEGCKTVV"
     gene            complement(2458..3621)
                     /locus_tag="VFX11_17725"
     CDS             complement(2458..3621)
                     /locus_tag="VFX11_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX4940516.1"
                     /translation="MNKNNEVYITGIGQSEIGKNLLRHPLLLTVDAVKEALEEAGLSI
                     AQIDGVSTYPGRVPQMLGFSPIGADELIDALGIKATWYAGGGEISSQLGAVVAAVGAI
                     RTGQARHVICYRTVYEAAALARPEEYPMPRRDRVEGSAQWTAPFNALSPATWFAQYAM
                     RHVKKYGLTREQLAQIALTDRANALLNPRALVREPLTMDEYLSSRMVADPLCLLDCDR
                     YTDASTVLIVSAGEALDEVKCTPIRIAAMAGSVDRHSWDQAEWMASYATGRDLWKRTD
                     YKPADVDTAQLYDGFSFLALTWLEALGFCEVGEGGRFIEGGKRIALDGELPLNTFGGQ
                     IAGGRLHGFGFAHEAVVQLRGLGGERQIKGNPKVAVATSGGGPLATALLLARD"
     gene            complement(3626..>3877)
                     /locus_tag="VFX11_17730"
     CDS             complement(3626..>3877)
                     /locus_tag="VFX11_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4940517.1"
                     /translation="NVAAKAVSGTGVVDTFTINHQKWHPKMEVPFVIARVALDDAPGV
                     YLTTNIVGCEVSEVDIGDRVKVTFEKQDDIFLPLFVKTA"
BASE COUNT          841 a         1161 c         1008 g          867 t
ORIGIN      
        1 gtgtcgacca ggagcgaagg acggccttgc tgcgttcagt ggtggaggca acccccggtt
       61 ggtccagggc tcagcaagag atagttacgg ccagcctgga tatcttctgg cagccgtcca
      121 cctatgaacg cctgatcaat ggctggaact tcaattccga acagtcgctg gcaactctca
      181 cctggctgat cgatctgatc gaaaccgccg tcaaggatga acggcgccct gaaatccagt
      241 aagaagccca acggaaaaac gggagccaag ctcccgtttt ctgtcaaccc gcctgaacct
      301 tcttggtgcc ttcctgcgca tgatgcgcaa acatcttcat caggtcaccg gacgtaatcc
      361 gccgcggaac ttcacctgcc gccaatggcg ccgcgccgcc gtaagaggtc ggcgtggtat
      421 cgaccttgtc cttcgcggcc tgcagacgca gcgcgcctac tgtcagatcc tgcttcttga
      481 cgtgcttgaa ggggtcgaag ttgaaccact tcatcgcgtt caggtgggtg atcttgttga
      541 tctgctcatc cttcagatgc ttgatcgtct tccacaggtg ttccggacat tcgggccaca
      601 gggtgtcaga gtgaggatag tcgcactcgt aggcgatcag atcttcattg agttcgtcca
      661 ggtttttcaa accataggga tcgtcgataa agcacagcat gaagtgcttc ttgaacactt
      721 cgctcggcag catcccgttg aagtggttat gggtccagaa cttgtggcgg gaatgggcga
      781 aatccgcgcg ctccatgaaa tacggtaccc agccaatgct ggcttccgac aacgcgatct
      841 tcaggttgta ttcgtgcagc gcggtaagat gcaaccagtc cgctgcgcca accgcgctgg
      901 acaacggcat ggtggtaatc cacgcctcga tcggtgtttc cattgaagca tgaggaaccg
      961 gattgccggc accgatatgc agacacaagg taatgtcatg ctcggcgatc gccttccaca
     1021 tcggattcca gtacgcgtta tgaacgctgg gcaattcctg cttggtcggg ttctcgttta
     1081 tggaaacggc cgtgcaacct ttcgcggcaa tacgcttgat ttcggcgacc gttgcatcca
     1141 tgtcccaggt gggcaacagg ccacaaggaa taaagcggcc gggatacgca ccacaccact
     1201 catcgatatg ccagtcgtta taggcgcgca ggtgtttcaa cgccagcttc ttgtccgctg
     1261 ccttgtgaaa acggccgcca tcgaaaccca cgcaggtacc aaagttaagc gacgccgcaa
     1321 cgccattgac gttcatatca ttgatacggg cgtgaacgtt gtagacgcct ttgcgcattt
     1381 cttccagcga attgggttcc atgccgtact cttccagagg acggccaacc accgcgttaa
     1441 gtcccaccga cggcatgtac ataccctggt atgtccagta actcttgcca tctttatcgg
     1501 tattgaattt gggcgcagtt ttcagatcat ctgccgacaa atgtttgtcg aacatatcag
     1561 gtggctcggt aatgtgatca tccacgctaa tgatcaccat atcgttcatt tccatgattc
     1621 tatcctctgg gtttgtctct acttgagttc tatttttcaa actctcaatt tgttttgtcg
     1681 ttggagaaat ccgggttcaa tgttctctgt caaatagcca ggtcaaacaa ccgattctct
     1741 gtcaaacaac ggtcttgcat ccctcaagtg ggcgccctgc cccgtggctg aaatcccgta
     1801 tctcgccaat ccgttttgcc actaccgccc gatcgggctg aaaggtgtaa cagcttggcg
     1861 gcattctggt ggcatccgac tgcttctcca gcgggatgat gcggccattc gccagcatat
     1921 tgccccactg cagcagacgc gccggctcct gcagcgcggg ttccagggtt tcgatccagg
     1981 accgcaactt gtccagtctt atgcctgccg cagcatcggg cttcacaatc tggaaacctg
     2041 attcgatcca ggccagcaac gtttcatcat tccgccgaac ttcagcgaga cgctggttat
     2101 cgaccgggtc cgaaaccaga acgtcataga tcgtctgtgc ccgatccgcc gcatgacttg
     2161 ctgccagcaa ggcaagatcg tacaaatgcg tgcagttggc ctttttttct ccccgctcgg
     2221 gaaacaacgc gagcgctacg ccagtaaatg tctgcaccag gccatcggcc gcccctggac
     2281 aggtcgacca gggtgcgcgg cgcatatccg gtacaattcg cagcgctttt ttgccgtcat
     2341 gatgtacaac gacactcatg cagtggaaat catcttccac ttctgcctgc acggcatttg
     2401 gcgacggtgt caaccggaaa cgacgccaga accccgtgtg ctgttgcgtc accttgatca
     2461 atcccgcgcg agaagcagtg ccgttgcgag cggcccgcct cccgatgttg cgacagcgac
     2521 cttgggattg cctttgatct ggcgctcgcc acccaaacca cgcaattgca cgaccgcttc
     2581 atgggcaaaa ccaaaaccgt gcagacgacc gcctgcgatc tggccaccga acgtattcaa
     2641 aggtaattca ccatccagtg cgatacgctt gccgccttcg atgaatcgcc cgccttcccc
     2701 tacttcacag aaacccaggg cttccagcca ggtaagtgcg agaaaactga aaccgtcgta
     2761 gagctgcgcg gtgtcgacat cggcaggttt gtagtccgtg cgtttccaca aatcccggcc
     2821 ggtcgcatag gaggccatcc attccgcctg atcccagcta tggcgatcca cgctacccgc
     2881 catcgcggca atgcgaatcg gcgtgcattt cacttcatcg agggcttctc ccgccgagac
     2941 aatcaggacc gtcgaggcgt ctgtgtagcg atcgcaatca agcaaacaaa gtgggtcggc
     3001 caccatgcgc gacgacaggt actcatccat cgtcagcggc tcacgcacca gggcgcgcgg
     3061 attgagcagc gcattggccc gatccgtcag ggcaatctgc gccagctgtt cacgggtcaa
     3121 gccgtatttt ttgacatgcc gcatcgcata ttgtgcaaac caggttgcgg gcgacaatgc
     3181 attgaacggt gcggtccatt gcgcggagcc ttccacccga tcgcgccgcg gcatgggata
     3241 ttcctcggga cgcgcaagag cagctgcttc atataccgtg cgataacaaa tgacatgacg
     3301 cgcctggccg gtgcgaatgg caccgaccgc cgccacaacc gcacccagct gcgaactgat
     3361 ttcgccgcca ccggcatacc aggtcgcctt gataccgagg gcatcaatca attcgtcggc
     3421 accgatgggt gaaaaaccca gcatttgcgg aacacgtcca ggataagtgg aaacaccatc
     3481 gatctgtgcg atggacaggc cagcctcttc cagcgcctct ttgacggcat ccactgtcag
     3541 cagcaacggg tgccgcagca gatttttacc aatctcggat tggccaatcc cggtgatata
     3601 gacttcgtta ttcttgttca tcaggctatg ccgtcttcac aaaaagcggc aggaaaatgt
     3661 cgtcctgctt ttcaaatgta acctttaccc ggtcgccgat atcgacttcg ctcacctcac
     3721 atcccacgat gttggtagtc agatacacgc cgggcgcgtc atccagcgca acgcgcgcaa
     3781 tcacaaaagg cacctccatc ttcgggtgcc atttctggtg attgatggtg aaggtatcca
     3841 ccacgccggt gccggaaacc gctttggctg caacatt
//
LOCUS       DASUEG010000941         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1495153, whole genome shotgun sequence.
ACCESSION   DASUEG010000941 DASUEG010000000
VERSION     DASUEG010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495153"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>2760)
                     /locus_tag="VFX11_17735"
     CDS             complement(130..>2760)
                     /locus_tag="VFX11_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX4940518.1"
                     /translation="GSSAQGLGEQEAARRQQEFGANRVETEARTPLWRSLLNEFSHFF
                     ALILWLAAGLAFLAEYLDPGTGMAKLGLAIVGVILINGFFSFWQAYRAEKALDALRSL
                     LPQNVNVLRDGSVTRIGTERLVPGDVVLLAEGDRVPADCRILESAGVRVNLSTLTGEA
                     QPLSRDANADTEVATNRLAARNLLLAGSLLVAGECKAVVYATGMRTEFGLIAHLTQTS
                     GETESPLQREIRRVSRLVALLALGLGLLFFVVGHAIGLPFWANLMFAIGIIVANVPEG
                     LLPTVTLSLALATQRMARRNALVRHLPAVETLGAASIILTDKTGTLTENRMTVRELYV
                     SGQHRLGARHWPRGGDLQLRAVARFCHSLRFTDGEPNGDPMEIALWQFAGPMPQQGQR
                     SSEIPFDGERKRMSVVTRSADGSGMLWCKGAPETVLPLCSHWIDSAATRPLDAAARQL
                     FSRAQNDMADRGLRVLALAWKPLAAGETESESQLELCGLVGLDDPARPDVHEAVTRCH
                     EAGVRVFMVTGDYPRTALAVAREVGIVREATPTVLTGAELAHMSPEALQLRLDAREIV
                     FARVTAEQKMQIVKAFQRKGEIVAVTGDGVNDAPALKTADIGIAMGLSGTDVAREAAD
                     IVLLDDHFATIVNAIEEGRAVFDNIRKFLTYILTSNIPELLPYLAFALFRIPLPLTII
                     QILAVDLGTDMLPAIALGAERPHPDVMKRPPRPRHERLLSWPVIARAYLFLGPLQALG
                     AFVGFFAVLKAGGWNYGDMPGREDAIYLQATTACMSAIVLAQMVNVFVCRHPQLPFWR
                     FGLRENPLLLLGLAVEAGVLIAIVYTAPGNQLFGTAPLPLDIWLTILPFALLLGVMEE
                     IRKGWCRKWCRKWCRKWSSG"
BASE COUNT          524 a          915 c          857 g          464 t
ORIGIN      
        1 ggttcgaccg gagccgctgc aagcggcgga ggaacgtcgg ccaaaatggc cgacggcccc
       61 gaaggggcga ggcgcaacgc gccgagtaat ccttcccccg tgcctttacc tttgccagat
      121 ccagattctt caaccactgc tccacttcct gcaccacttc ctgcaccact tcctgcacca
      181 ccccttccga atctcctcca tcacacccag cagcagcgca aaaggcagta tcgtcagcca
      241 gatgtccagc ggcagcggcg ccgtgccaaa cagctggttg ccaggcgccg tataaacaat
      301 cgcgatcaag acaccggcct ccaccgccag ccccagcagc agcaacggat tctcccgcag
      361 cccgaagcgc cagaagggca gttgcggatg ccggcacacg aagacattga ccatctgtgc
      421 cagcacgatt gcgctcatgc acgcggtggt ggcttgcagg tagatcgcgt cctcccggcc
      481 aggcatgtca ccataattcc agccaccggc tttcagcacc gcgaaaaagc cgacgaacgc
      541 gcccagtgcc tggagcgggc caagaaacag gtacgccctg gcaatgaccg gccatgacag
      601 caggcgctcg tgccgtggcc gcggcgggcg tttcatcacg tccgggtgag ggcgctcggc
      661 acccagcgcg atggccggca gcatgtcagt gccaagatcc accgccagga tctggatgat
      721 ggtgagtggc agcgggatac ggaacagcgc gaaggcaagg tagggcaaca gttccgggat
      781 gttggacgtc aggatgtagg tgaggaattt gcggatattg tcgaaaactg cgcggccttc
      841 ctcgatcgca ttgacgatgg tggcgaaatg atcgtcgagc agcacgatgt cggcggcctc
      901 gcgcgcaaca tcggtgccag aaagccccat cgcaatgcca atgtcagcgg ttttgagtgc
      961 aggggcatcg ttgacgccgt cgcccgtcac cgcaacgatt tcgcccttgc gctgaaacgc
     1021 ctttacgatt tgcattttct gttcggcggt gacgcgggca aacacgattt ccctggcatc
     1081 gagccgcagt tgcagcgctt ccgggctcat gtgggccagc tccgcgccgg tcagcaccgt
     1141 gggtgtagct tcccgcacga tgccaacctc gcgcgccacc gccagtgccg tgcgcggata
     1201 atcaccggtg accatgaaca cgcgcacgcc ggcctcatgg cagcgggtga cagcttcatg
     1261 cacgtcgggc cgcgcgggat cgtccaggcc gacgaggccg cacaattcca gttgcgactc
     1321 ggactcggtc tcacccgctg caagcggctt ccatgccagc gcgagcaccc gcaggccgcg
     1381 gtcggccatg tcgttctggg cgcggctgaa cagctgccgg gcggcagcgt cgagcgggcg
     1441 cgtggctgcg gaatcgatcc agtggctgca caacggcagt acggtttcgg gggcgccttt
     1501 acaccacaac atgccgctgc cgtcggcaga gcgcgtcacc accgacatgc gcttgcgctc
     1561 accgtcaaac ggaatttcgc tgctgcgctg accctgttgc ggcatcggcc cggcaaattg
     1621 ccagagcgca atttccatgg gatcgccgtt gggctcgcca tccgtgaacc gcaggctgtg
     1681 gcagaaccgg gcgacggcgc gcagttgcag gtcaccgccc cgcggccagt gtcgggcgcc
     1741 cagccggtgc tgaccggaca catacagctc ccgcacggtc atccggtttt cggtcagtgt
     1801 tccggtcttg tccgtcagaa tgatgctggc ggcgccgagt gtctctactg ccggcaggtg
     1861 gcgcaccagc gcgttgcgtc gcgccatgcg ctgcgttgcc agcgccaggg aaagcgttac
     1921 cgttggcaac agaccctcgg gcacgttggc cacaatgatg ccgatcgcga acatcaggtt
     1981 ggcccagaac ggcaggccaa tggcgtggcc gacaacgaag aagagcaggc caagccccag
     2041 tgccagcagg gcaaccaggc gtgaaacgcg gcggatttcc cgctgcagtg gcgattcggt
     2101 ttcgccggac gtttgcgtca gatgcgcaat aagtccgaat tcggtacgca ttccggtggc
     2161 gtagaccacg gccttgcact cgcctgccac gagcagcgag cccgccagga gcaggttgcg
     2221 agccgccagc cggtttgtgg caacctcggt atccgcattg gcatcgcgcg acaggggctg
     2281 cgcttctcct gtcagcgtcg agagattgac ccggacgccc gcactttcga gaatccggca
     2341 atccgcaggc accctgtccc cctcggccag cagcacgaca tcgccgggta ccagcctttc
     2401 ggtgccgatt ctggtgacgc tcccgtcacg cagcacgttg acgttctgcg gcagcagcga
     2461 gcgcaaggca tccagcgctt tttcggcgcg atatgcctgc cagaaagaaa agaagccatt
     2521 gatcaggatg acgccgacga tcgccagacc cagcttggcc atgccggttc cgggatcgag
     2581 atattccgcc agaaaggcca gcccggccgc cagccagaga atgagggcaa aaaaatgact
     2641 gaactcgttc agcagcgagc gccagagcgg cgttctggct tcggtttcaa cccggttggc
     2701 gccgaactcc tgctgtcgcc gcgccgcttc ctgttcgccc agcccctgcg cgctgctgcc
//
LOCUS       DASUEG010000942         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1714593, whole genome shotgun sequence.
ACCESSION   DASUEG010000942 DASUEG010000000
VERSION     DASUEG010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714593"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFX11_17740"
     CDS             complement(<1..443)
                     /locus_tag="VFX11_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX4940519.1"
                     /translation="MMNKWFCSAAVGASLMIAGCTTDPYTGEQKVSKAAMGATAGALL
                     GAAVSSKDDRAKGAVIGAAVGGGAGYYFDRQEQILRQKLQGTGVSVTRTQTGIILNMP
                     GNISFPSGQYAILPSFYDVLNSVSLVLKEFDKTAVKVSGHTDSTGG"
     gene            complement(584..742)
                     /locus_tag="VFX11_17745"
     CDS             complement(584..742)
                     /locus_tag="VFX11_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940520.1"
                     /translation="MDPKSKEIYNKIENYLEERKKRDRRDEQQAAQQAQISEEDRRKK
                     RDRRTSAE"
     gene            1000..2025
                     /locus_tag="VFX11_17750"
     CDS             1000..2025
                     /locus_tag="VFX11_17750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4940521.1"
                     /translation="MRILVTGGFGFVGSKAVLGLLQKGHHVRVLELPGHPLQQQFADF
                     WAAAGPVDFVPGDVAVYRDVEGAMTGCDRVLHTAALLNSVASFSRFHRVNALGTANIC
                     QASLAAGIARLVVVSTSDVFGIPEPGQTLDENSPLQPWNEPYADTKIEAVQTARHYRD
                     QQGLPVTLVYPGWVYGPGDRQFFPAVLDMIRDRHVFTWEKGNPYEINLTFIDDLVDAL
                     LRCLLTHEQNGEYLILDTQTGMTPERLYRTLADHIQLPIRVHHLPYSLMMLVAACSQW
                     LARRGFIAKSLLSTTDVKAFGNEFHFSNRRAAEQLGWRPATPVAEGLRRSLEWQAKRR
                     ENPDLVG"
     gene            2059..2409
                     /locus_tag="VFX11_17755"
     CDS             2059..2409
                     /locus_tag="VFX11_17755"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEX4940522.1"
                     /translation="MLAGLLQLLLFYLLGFIVATALHLPIPAAVVGLILLLMFLMARR
                     SASESLLDATGKLLPFLPLCLIPASAGVIRYGALLEKEWLALTAAVVISTLASFVITP
                     YIFRFYRRVLGDSR"
     gene            2406..>2817
                     /locus_tag="VFX11_17760"
     CDS             2406..>2817
                     /locus_tag="VFX11_17760"
                     /inference="COORDINATES: protein motif:HMM:NF016088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEX4940523.1"
                     /translation="MSTLIDWITAFPLFGLLLTLLTFYCGQRLYIASGRNAWLQPVVA
                     SMVLVILVLVLADIPYERYYRSAEMLHLLLGPATVALAVPLFLHARRIRALLLPILLT
                     LITGSVLTVGIAVGVLWLMGGSESSLLSITTKSIT"
BASE COUNT          544 a          801 c          805 g          667 t
ORIGIN      
        1 ccgccggtac tgtcggtatg gcccgacacc ttgacggccg tcttgtcgaa ttccttcaga
       61 accagtgaca cgctgttcag cacgtcatag aacgacggca gaatcgcgta ctggccactg
      121 gggaagctga tgttgccggg catgttgagg atgatgccgg tctgggtgcg ggtaacgctg
      181 acaccggtgc cttgcagttt ttgccgcagg atctgctcct ggcggtcgaa gtagtaaccg
      241 gcaccgccac ccacagccgc gccaatcaca gcccccttgg cccgatcgtc cttgctggag
      301 acagcagcac ccagcagcgc gccagcggtg gcacccatgg cagccttgct gaccttttgt
      361 tcacccgtgt acgggtcagt ggtacaaccg gcaatcatca gggatgcgcc tacggcagca
      421 ctgcaaaacc atttgttcat catcttctcc tgtcagtggg tattgtgacg atatggtttg
      481 aaagtgttgc cgattctaca ttaacccgtc ctgaatgagt ccagcatgaa aacgaaaaag
      541 cggagactgt gctccgcttt ttcaataata ccgggactgg ggcttattca gcgctggtgc
      601 gacggtcccg cttcttgcga cgatcttctt cactgatctg cgcctgctga gctgcctgtt
      661 gctcatcacg gcgatcccgc tttttgcgct cctccaggta attttcaatt ttgttgtaga
      721 tttctttgga cttgggatcc atgactgcac tcctatttga gacgagtctc ttgacgataa
      781 cgaaacgggc cgcaacaagc ggccaatgga taagttttaa ggatcaggtc gaaataagta
      841 tagtccccga catccgatct ggaatggatc taatcccgtt ttttagatca aatggcaata
      901 tttgaaataa ccaaaaagaa taattcaacg cacatcaagc actgccatgg ggcataaaag
      961 gtgctgtata gtacgtccgt accattttta gggggatata tgcgaattct ggtgactggc
     1021 gggtttggtt ttgtgggcag caaggcggta ctcgggctgc tgcagaaagg acatcacgtg
     1081 cgcgtactgg aactgccagg acaccctttg cagcagcagt tcgctgactt ctgggctgcg
     1141 gcggggccgg tcgatttcgt gccgggggat gtggccgttt atcgcgatgt ggaaggggct
     1201 atgacgggtt gcgatcgggt gttgcatacc gctgcccttc tcaattccgt tgccagcttc
     1261 agccgctttc accgggtcaa tgcgctgggc actgcgaaca tctgccaggc cagcctggca
     1321 gcgggaatcg cccgtctggt ggtggtcagc acctcggacg tatttggcat tcctgagcca
     1381 gggcaaacgc tggatgaaaa ctccccctta caaccctgga acgagcccta cgccgacacc
     1441 aagatcgagg ccgtgcagac cgcacggcac taccgcgacc agcaagggct tccggtaacc
     1501 ctggtatatc caggctgggt gtatggacct ggcgatcgtc agtttttccc ggcggtactg
     1561 gacatgatca gggatcgcca tgtcttcacc tgggaaaagg gcaatcccta cgaaatcaac
     1621 ctcaccttca ttgatgacct cgtggatgcc ctgctccgct gcctgctcac ccatgagcag
     1681 aacggtgaat acctgattct ggacacacag acaggcatga caccggaaag actgtatcgc
     1741 accctggctg accatatcca gctaccgata cgggtccacc atttgcccta ctcattgatg
     1801 atgctggtag cagcctgctc gcaatggctg gcacggcggg gtttcattgc caagtcgctg
     1861 ctcagcacca cggatgtgaa ggctttcggc aatgaattcc acttcagtaa ccgccgcgca
     1921 gcggagcaac tgggctggcg gccagccacg cccgttgctg aaggcctgcg gcggagtctg
     1981 gagtggcagg ccaagagacg ggaaaatcct gatctggtag gctagcggcc ccattccttc
     2041 ctcaggagcc tttgcacgat gctggccgga ttgctgcaac tgctgttgtt ttacctgctg
     2101 ggtttcatag tggccaccgc gctgcatctg ccgattccgg ctgcggtagt ggggctgatc
     2161 ctgttgctga tgtttctcat ggcgcggcgt tccgccagcg agtcgttgct ggatgccacc
     2221 ggcaagctgc tgccgttcct gccgttgtgc ctgatccctg ccagtgccgg cgtcatccgc
     2281 tatggcgcgc tgctggaaaa ggaatggctg gctctgaccg cagccgttgt aatcagtacg
     2341 ctggcctcgt tcgtcatcac gccctatatc ttccggtttt accggcgtgt gctgggagat
     2401 tcacgatgag cacgttgatt gattggatca cggcctttcc gctgttcgga ctgctgttga
     2461 cgctgctgac cttttactgc ggccagcgcc tgtacattgc cagcggccgc aatgcgtggt
     2521 tgcaaccggt ggtcgcgagc atggtgctgg tgattctggt gctggtactg gccgacattc
     2581 cctacgagcg ttattaccgc agtgcggaaa tgctgcatct gctgctgggt cctgccaccg
     2641 ttgccctggc ggtccccctg tttctgcatg cccgacgcat ccgcgcgctg ctgttaccca
     2701 ttctgctcac actgataaca ggctcggtgc tgacggtggg aattgccgtc ggtgtgctct
     2761 ggctgatggg gggatcagaa tccagcctgc tgagcatcac caccaaatcc atcacca
//
LOCUS       DASUEG010000943         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_271789, whole genome shotgun sequence.
ACCESSION   DASUEG010000943 DASUEG010000000
VERSION     DASUEG010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271789"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFX11_17765"
     CDS             complement(<1..347)
                     /locus_tag="VFX11_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4940524.1"
                     /translation="MPEHERAAVTVSGERLALSAAQLGIWIGQQLDRNNPAYWTAEAV
                     ELQGELDANRFAQAVQNTVNECEALQMHFHCEGEQVWQEPARTTQTIWQILDFTAAST
                     DAWQAAQKWMQIQL"
     gene            complement(340..1215)
                     /locus_tag="VFX11_17770"
     CDS             complement(340..1215)
                     /locus_tag="VFX11_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX4940525.1"
                     /translation="MSIPRIATYILPDTQSLPANRVDWQADPQRALLLIHDMQDYFLD
                     FFDVGAEPVSTLVENILRLRRWCDGAGVPVLYTAQPPQQSAQERGLLQDWWGPGITAA
                     PERAPIYSPLAPRPQDTVLTKWRYSAFARSDLQQRMQDMRRDQLIICGIYAHIGCMTT
                     AVEAFMHDIQPFFVADAVADFSLLQHRMALDWVSQRCGLVLSTDQLIRQLCPPPPLPQ
                     SLDELRGQVAALLQLNPSDLQPDDDLLLMGLDSIRLMSLVENLRHAGADIEFVDLAER
                     PTLAEWWERLGAIYA"
     gene            complement(1225..3036)
                     /locus_tag="VFX11_17775"
     CDS             complement(1225..3036)
                     /locus_tag="VFX11_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940526.1"
                     /translation="MSTLELQGWTPWPEDFARLYRELGYWRGQTLADLLADRAHHQPQ
                     RLALVCGERRWTYRELHRQSLQLALGLHRLGIQRQDRVIVQLPNVAEFFTVCFALFRL
                     GALPVFALPAHRRAEITHFCELAQAKAWIVAERDGGFDFRELARQVASAVPSLQQVVV
                     WGDAGEFIALDSLLATVDEPAFSQTVLSGTDFTGTAFSATSPQDDTAFVEQALVEKSS
                     VEQALPSGPAASDVAFLQLSGGSTGLPKLIPRTHDDYLYSVRASASICALDQNSVYLC
                     ALPAAHNFPLSSPGTLGTFYVGGTVVLARRPAPDDCFPLIAKESVTVTALVPPLAMVW
                     LDAASKRRSDLHSLQLLQVGGARFSAEAARRVKPVLGCRLQQVFGMAEGLVNYTRLDD
                     SDDVIATTQGRPISPADEIRVVDDEDQPVLPEEPGHLLTRGPYTIRGYFRADEHNRRA
                     FTADGFYRTGDIVRQLPGGHLVVEGRAKDQINRGGDKVAAEEVENHLLAHPAVHDAAL
                     VAMPDAFLGEKTCAFVVIRGDAPRAIELLRFLQERGLAGYKIPDRIEFIAQLPQTGVG
                     KVNKRELRERIAQLLGATAVYQIAPVKNKALQTASAE"
     gene            complement(3033..>4169)
                     /locus_tag="VFX11_17780"
     CDS             complement(3033..>4169)
                     /locus_tag="VFX11_17780"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX4940527.1"
                     /translation="FVSPRQAVLAAGVALQRQMTGLDQVPDMASQLLQEARQQGWSRA
                     LLMGAIPFHPQASARLFVPRYVEVLPRLESLTATTETAPTRRTVRMDRTRMQPTPHQY
                     KQLVKKAVSQIRKGDFEKVVLSRSVHLQAEVDLPALLSTLAQRNPLGYTFALNLGDDA
                     AQARSLVGASPELLLSKCGNRVLSNPLAGSVPRSQDAEENRRRADALLQSQKDRHEHA
                     LVVDAVAAALRPFCRALEVPAVPSVLATPTMLHLSSEISGELIDPTLSSLELALALHP
                     TPAVCGFPPQPALDFIQHNEGFDRGLFTGLVGWSGAHGDGEWAVTIRCAEVTADSVTL
                     YAGAGVVEGSDPVLELAETTAKMRTMLSALGLEPGLESVLGALA"
BASE COUNT          770 a         1364 c         1290 g          745 t
ORIGIN      
        1 agttgtattt gcatccattt ctgcgctgcc tgccaggcgt cggtactggc ggcagtgaaa
       61 tccagtatct gccagattgt ctgcgtcgtg cgcgccggtt cctgccacac ctgctcgccc
      121 tcgcagtgaa aatgcatctg cagggcttcg cattcgttca cggtattctg cacagcttgc
      181 gcaaaccggt tagcgtccag ttcaccctga agttccactg cttccgccgt ccagtaggca
      241 ggattgttgc ggtccagttg ctggccgatc cagatgccca gctgcgcggc ggacaaagcc
      301 agtcgctcac cggaaacagt aacagcagcg cgctcatgct caggcataaa tcgcacccag
      361 ccgttcccac cattccgcca gcgtcggccg ctccgccaga tcgacaaatt caatgtccgc
      421 gccggcatgg cgcaggtttt ccaccaggct catcagccgg atcgaatcca gccccatcaa
      481 cagcagatca tcgtcgggct gcaggtcgga cggattcagc tgcagcagcg ctgccacctg
      541 cccgcgcaat tcatccagcg attgtggcag cggcggaggc gggcataatt gacggatcag
      601 ttgatccgtg gacagcacca ggccacagcg ctgcgacacc cagtccagcg ccatgcgatg
      661 ctgcagcagc gaaaaatcgg cgacggcatc ggccacaaaa aacggctgga tgtcatgcat
      721 gaaggcttcc acggccgtgg tcatgcaacc gatatgagcg tagatgccgc agatgatgag
      781 ctggtcgcgt cgcatgtcct gcatgcgctg ttgcagatcg ctgcgggcga aggcgctgta
      841 tcgccatttg gtgagcacgg tgtcctgcgg gcgaggtgcc agcggggaat aaatcggtgc
      901 gcgctcgggc gccgctgtta tgcctgggcc ccaccagtcc tgcaacagcc cacgttcctg
      961 tgcggattgt tgcggcggtt gtgcggtgta tagcaccggc actccggcgc catcgcacca
     1021 gcgacgcagc cgtagaatat tctccaccag cgtgctgacc ggttccgcgc ccacatcaaa
     1081 aaaatccaga aaataatcct gcatgtcgtg aatcagcaac agggcgcgct gcggatcggc
     1141 ctgccagtcc acgcggttgg cgggcaggga ttgagtgtcc ggcagaatat aagttgcaat
     1201 gcggggaatg ctcatggtgt gggtttactc cgctgatgct gtttgcaggg ctttgttttt
     1261 cacgggcgcg atctggtaga ccgcagtggc gcccagcagc tgggcaatgc gttcgcgcag
     1321 ttcgcgcttg ttcaccttgc ccacgccggt ttgcggtagc tgggcaatga attcgatgcg
     1381 gtcgggaatc ttgtagccgg ccaggccgcg ttcctgcaga aaacgcagca gctcgatggc
     1441 gcggggtgcg tctccgcgaa tcaccacgaa ggcgcaggtt ttttccccca ggaacgcatc
     1501 gggcatggcc accagtgccg catcgtgcac ggccgggtgc gccagcaaat gattttccac
     1561 ttcctctgct gccaccttgt cgccgccgcg attgatctgg tccttggcgc ggccttccac
     1621 gaccagatgg ccgccgggca gttgccgcac gatatcgccg gtgcggtaga agccatccgc
     1681 cgtgaacgcg cgccggttgt gttcgtcggc gcggaaatag ccgcgaatgg tgtatgggcc
     1741 gcgtgtaagc aggtggcccg gttcctccgg cagcaccggc tggtcttcgt cgtcgacaac
     1801 gcggatttca tcggcggggg aaatggggcg gccttgcgtg gtggcgatga cgtcatcgct
     1861 gtcgtccagg cgagtgtaat tcaccagtcc ttccgccatg ccgaacacct gctgcaggcg
     1921 acagcccagc acgggtttga cgcgacgcgc ggcttcggcg ctgaagcggg cgccgcccac
     1981 ctgcaacagc tgcaggctgt gcagatcgct tctgcgtttg ctggcagcgt ccagccacac
     2041 catcgccagc ggaggcacca gtgccgtcac cgtcacgctt tcttttgcaa tcagcggaaa
     2101 gcagtcatcc ggcgcggggc ggcgcgccag caccacagtt ccgccgacat aaaaggtgcc
     2161 cagcgtgccc ggcgaactca acggaaagtt gtgcgcggcg ggcagcgcgc acaaatacac
     2221 gctgttttga tcgagcgcgc agatgctggc actggcgcgc acgctataaa ggtaatcatc
     2281 gtgcgtgcgc ggaatcagtt tcggcagacc ggtgctgccg ccggacagtt gcaggaacgc
     2341 gacgtcgctg gcagcagggc cgctcggcag tgcctgttcc accgaagact tttccaccaa
     2401 tgcctgctcc acaaaagcag tgtcatcctg cggcgacgtt gctgagaaag cagttccagt
     2461 aaaatcggtt cccgataaaa ctgtttgcga aaaagccggc tcatccaccg tcgccagcaa
     2521 cgaatccagc gcgataaatt cccccgcgtc accccacacc acaacctgct gcagagacgg
     2581 cactgcactc gctacctgcc gtgccagttc gcggaaatca aaaccgccat cccgctccgc
     2641 cacgatccag gccttcgcct gcgccagttc gcagaaatgc gtgatctcgg cgcggcggtg
     2701 ggcgggcagg gcaaacaccg gcagcgcgcc caggcggaac agcgcaaagc acaccgtgaa
     2761 aaattcggcg acattgggca actgcacgat cacgcgatcc tgccgctgaa tgcccagccg
     2821 gtgcagcccc agtgccagtt gcagcgactg ccggtgcaat tcccggtacg tccagcgccg
     2881 ctcgccgcac accagcgcca gacgctgcgg ctgatggtgc gcgcggtctg ccagcagatc
     2941 cgccagggtt tggccgcgcc aatagcccag ttcgcggtac aggcgggcga agtcctcggg
     3001 ccagggtgtc cagccctgca attccagtgt gctcacgcca gcgctcccag cacggattcc
     3061 aaacccggtt ccaggcccag tgcgctcagc atcgtgcgca ttttcgcggt ggtctccgcc
     3121 agttccagca cagggtcaga cccttccacc acgccggcgc ctgcatacaa ggtcacactg
     3181 tccgccgtca cttcagcgca gcggatggtg acggcccact cgccatcgcc gtgggcaccg
     3241 ctccagccca ccaggccggt gaacaggcca cgatcgaaac cttcattgtg ctgaatgaaa
     3301 tccagcgccg gctgcggcgg aaaaccgcac acggcgggcg tcgggtgcag cgccagcgcc
     3361 agttccagcg acgacagcgt gggatcgatc agctcgccgc taatttcgct ggaaaggtgc
     3421 agcatggtgg gcgtggccag taccgacggc acggccggca cctccagcgc gcggcagaag
     3481 gggcgcaacg cggcagccac cgcgtccacc accagcgcgt gttcgtggcg gtccttttgc
     3541 gattgcagca gcgcatcggc gcggcgccgg ttttcctcgg catcctggct gcggggcacc
     3601 gaaccggcca gcggattgga caacacgcgg ttgccgcatt tcgacagcag cagttccggg
     3661 ctggcgccca ccagcgagcg ggcttgcgca gcatcgtccc ccagattcag cgcgaaggtg
     3721 taacccagcg gattacgctg ggccagcgtc gacagcaacg ctggcagatc cacctcggcc
     3781 tgcaggtgca ccgagcgcga cagcaccacc ttttcgaaat cgcccttgcg gatctggctc
     3841 accgcctttt tcaccagctg tttatattgg tgcggcgtcg gctgcatgcg ggtgcggtcc
     3901 atccggacgg tccggcgtgt gggtgccgtt tccgttgtgg ccgtcaggga ttccagtcgc
     3961 ggcagcactt ccacatagcg cggtacgaac agccgggcag acgcctgcgg atgaaacggg
     4021 atcgcgccca tcagcagcgc gcggctccag ccttgctggc gggcttcctg caacagttgg
     4081 gatgccatgt ccggcacctg atccagcccc gtcatctgtc gctgcagggc cacgccggcc
     4141 gccagcaccg cctggcgggg ggacacaaa
//
LOCUS       DASUEG010000944         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1582295, whole genome shotgun sequence.
ACCESSION   DASUEG010000944 DASUEG010000000
VERSION     DASUEG010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582295"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFX11_17785"
     CDS             complement(<1..578)
                     /locus_tag="VFX11_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4940528.1"
                     /translation="MRTNTNNYPSPVRAWLTVFILMLAYVLSFVDRQILNLLVGPIRQ
                     DLQISDTEMSLLMGFSFAVFYTICGIPLGRLADSRSRRGLIAAGVVFWSVATATCGLA
                     RSYWQFFLARIGVGVGEAALSPAAYSLITDSFPPAKRATALSAYSMGIYIGSGMAFLL
                     GAKVIQFALSQGEIVLPLLGTIRPWQLIFMLLG"
     gene            889..1671
                     /locus_tag="VFX11_17790"
     CDS             889..1671
                     /locus_tag="VFX11_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940529.1"
                     /translation="MEMRNQQEAMKVVQKQESSSGLAAGAQGWSRRLMLPALLACAAF
                     STNSYALLMSQDSSYGTGSITVDTDTGLQWLDPWLGNAGGSFSSFNAVKTQFASGGYF
                     EGFRYATRLEVETLLFSSVGINAVTSQSSGTATSSDIQGVANLMSFFDNTFSQVFGPD
                     AYISILDAVFDDGAGGADETVLTSSKFGPSLGGGTVQFYDSPDLDSNTNPYGHWLVRD
                     INIASSSVNVDEPQGLLLLGIALAAAFGFAGRKRSTQAIVAA"
     gene            complement(1746..>3151)
                     /gene="glmS"
                     /locus_tag="VFX11_17795"
     CDS             complement(1746..>3151)
                     /gene="glmS"
                     /locus_tag="VFX11_17795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX4940530.1"
                     /translation="DLMGAVRATTAKLRGAYALGVMTGKDSTRLIAAREGSPLVLGVG
                     LGENFIASDQLALLPVTTRFIFLEEGDIAEITREHIRIEDSAGRPAERGIKQYIGGDT
                     SADKGEYRHFMLKEIFEQPTAIRATLEGRTSIDGVNVDIFGPDAKNIFPKIEAVQIVA
                     CGTSFHAGLVAKYWFEGLAGVQCSVEVASEFRYRKTVVRPNTLFVTISQSGETADTLA
                     ALRDAKGYLSTLTVCNVPTSSLVRESAMAFMTKAGPEIGVASTKAFTTQLTALYLLVV
                     GVGKFHAINATEEKRLLLGLQQLPDVVQSALRLNKPIEQMAEMFAEKHHALFLGRGVQ
                     YPVALEGALKLKEISYIHAEAYPAGELKHGPLALVDADMPVVTVAPSDSLLEKLKSNL
                     QEVRARGGELFIFQDAEAGVEKQKDVHVLDVPKVAPELAPIVYTIPLQLLSYHVAIIK
                     GTDVDQPRNLAKSVTVE"
BASE COUNT          672 a          943 c          869 g          667 t
ORIGIN      
        1 cccagcagca tgaatatcaa ctgccaggga cggatggtac ccagcaacgg cagcacgatc
       61 tccccctgcg acagcgcaaa ctggatcacc ttcgcgccca gcagaaatgc catgccggag
      121 ccaatgtaaa tgcccatgga ataggcgctc aacgcggtgg cgcgcttggc gggcggaaaa
      181 ctgtcggtaa tcagcgaata cgccgcaggc gacaacgcgg cttcgcccac tcccacgccg
      241 atgcgggcca gaaaaaattg ccagtagctt cgggccaggc cgcaggtggc ggttgccacg
      301 ctccagaaca ccacgcccgc cgcaatcagc ccgcgccggc tgcgggaatc cgccagtcgt
      361 cccagcggga tgccgcagat ggtgtaaaac acggcgaagg aaaagcccat cagcaggctc
      421 atttcagtgt cggaaatttg caggtcctgc cggatcggtc ccacgagcag attgagaatc
      481 tggcgatcga cgaacgagag cacatacgcc agcatcagaa tgaacacggt cagccacgcc
      541 cggacgggtg acggataatt attggtatta gttcgcaatt gatgtctcct gcgggcttgc
      601 agcctttact gcagcctagc aggtattatt attgcgccat gcaggcagat cggcgcttgg
      661 ccgctccagc cagatttact gccactcgcc tccggttttg aacgctctgc tgaataatgc
      721 gtcgcgccag tgtcgaattt cttaacagac gctgacaaaa tttcctggat tcgagcgcta
      781 tgatactgat taataatgcc atatgatcag tggaacgtgg tttgcttatt caagcgcact
      841 ttcctgaatt ggctataaaa acaagagcat tgcaacggag tggtgaacat ggaaatgcgg
      901 aatcaacagg aggcgatgaa agtggtgcag aaacaggaat catccagcgg attggcagct
      961 ggagcccaag gctggagccg tcgcctgatg ttacccgcat tgcttgcatg tgcggcattt
     1021 tcaaccaatt cctacgcgct gctgatgagc caggattcca gctatggcac gggctccatt
     1081 accgttgaca ccgataccgg attgcagtgg ctggatccgt ggctgggtaa tgcaggcggc
     1141 agcttcagtt ccttcaatgc cgtgaaaacc cagttcgcca gcggcggcta tttcgaggga
     1201 tttcgttacg ccacccgcct ggaagtcgaa accctgctct tctcctcggt cggcatcaac
     1261 gcagtcactt cgcaaagctc cggcactgcc acttccagcg acatccaggg tgtggccaat
     1321 ctgatgtcgt tcttcgacaa cacgttttcg caggtcttcg gtcctgacgc ctacatcagc
     1381 attttggacg ccgtatttga cgatggcgcc ggcggtgcag acgaaaccgt attgacgtcc
     1441 tcgaagtttg gcccaagcct cggcggtggc actgtgcagt tctacgacag cccggacctc
     1501 gacagcaata ccaatcccta cggccactgg ctggtacgcg atatcaatat tgccagcagc
     1561 agcgtaaacg tcgatgaacc gcaaggcctg ttgctgttgg gcatcgccct ggcagccgca
     1621 ttcggttttg ccggccgcaa gcgttcgacc caagccatcg ttgccgcctg aaatacaaag
     1681 gggtaatggc agcaccttgc caccattacc ccctgcattc gaaaatttct ttcgttccgc
     1741 ccgtcttatt ccactgtaac ggacttcgcc aaattccttg gttgatccac gtccgtgccc
     1801 ttgatgatcg ccacgtgata gctcagcaac tgcaacggaa tggtgtacac gatcggcgcc
     1861 agttccggcg ccactttcgg cacatccagc acgtgcacgt ccttctgctt ctccacgccc
     1921 gcttccgcgt cctggaaaat aaagagttca ccaccgcgcg cgcgcacttc ctgcaggttg
     1981 gatttcagtt tttccagcag tgaatccgac ggcgccactg tcaccaccgg catatccgca
     2041 tccaccagcg ccagcgggcc atgtttgagc tcaccggccg gataggcttc cgcgtgaata
     2101 taggaaattt ctttcagctt gagcgcgcct tccagcgcca ccggatactg aacaccgcgc
     2161 cccaaaaaca acgcgtggtg tttttcggca aacatttccg ccatctgttc aatcggttta
     2221 ttcaggcgca atgctgactg gaccacgtct ggcaactgtt gcaggcccag cagcaggcgt
     2281 ttttcttccg ttgcgttgat cgcgtggaat ttgcccacgc ccaccaccag cagatagagc
     2341 gcagtgagct gggtggtgaa cgccttggtg gacgccacgc cgatttcagg gccggccttg
     2401 gtcatgaacg ccatggcgga ttcacgcacc agcgaactgg tgggcacgtt gcacacggtc
     2461 agcgtggaca ggtaaccctt ggcatcgcgc aaggctgcga gagtgtcagc ggtttcgccg
     2521 gactgcgaga tggtgacgaa caatgtattg gggcgcacca cggttttgcg gtagcggaat
     2581 tcgctggcca cttcgacgct gcactgcacg cccgccagac cttcaaacca gtattttgcc
     2641 accaggccag catgaaagga cgtgccgcag gcaacgatct gcaccgcttc gattttcggg
     2701 aagatgtttt tggcatccgg gccgaaaata tccacattca cgccgtcaat gctggtgcgt
     2761 ccttccagcg tggcgcggat cgcggtgggc tgctcgaaga tttccttcag catgaagtga
     2821 cggtattcac ctttgtcggc ggaggtgtca ccgccaatgt actgcttgat accgcgctcg
     2881 gcgggacggc ctgcgctgtc ctcgatgcgg atatgttcgc gggtgatctc agcgatgtcg
     2941 ccttcttcca ggaaaatgaa gcgggtggtc accggcagca atgccagctg gtcagacgca
     3001 atgaagtttt cgcccaggcc cacacccaat accagcggac tgccttctcg tgcggcaatc
     3061 aggcgggtgg aatccttgcc ggtcattacg cccagcgcat aggcaccacg cagtttcgct
     3121 gtggtggccc tcaccgcgcc catcagatcc a
//
LOCUS       DASUEG010000945         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1763696, whole genome shotgun sequence.
ACCESSION   DASUEG010000945 DASUEG010000000
VERSION     DASUEG010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763696"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1623
                     /locus_tag="VFX11_17800"
     CDS             262..1623
                     /locus_tag="VFX11_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4940531.1"
                     /translation="MNSLIDRILNWGWQPELHSAIQMRRIRTLSGSALLLVLVGIPFW
                     MRAIEWQIALRMVTVPTAIALTLAGLVLLRWRHTARMHFLCTQLISLALYVAGMGAML
                     SSGGLGNITEGWLMLVPVVAGINLGLRPALQWGAVILLTLVAVALLPSLGYVLVDQTP
                     PSHQASQQVLQGLGVFVALMILVSSFVSQVSHSEAVLAQQNLQLQQQIIEKEKAEREA
                     RSAEQAKTRFLANMSHEMRTPLNAILGFSRRLDKSLAGRLDEREHNALSRIAECGEGM
                     LRLVDDLFDLSAIDAGTLILHRQPVALGPLLARVVAELQPLAQQRGLSLQQGQCPDCC
                     VTGDPERLEQVLTSLLRFGLTSTQAGDVRISAHRQEDAQVCVSIEDSAVPLDEAAQLR
                     LLDRYNHLHSRGWQDLDGFALGPALAVELIRLHGGRIQIGPGSLGGNCFRILLPPHAP
                     AHE"
     gene            complement(1703..2089)
                     /locus_tag="VFX11_17805"
     CDS             complement(1703..2089)
                     /locus_tag="VFX11_17805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4940532.1"
                     /translation="MRAATKAMVAGLTLAGTLTSLPGQCGLMAADNLQAMSAQNAPVA
                     HVFTPSAPDDSLIALALDHPPEPDFVSRAVPALDTHPLLYPAGDTASILPGSGTLRFG
                     PMPEPGTLSLFGLGLLVWAFIQHRRR"
BASE COUNT          394 a          691 c          736 g          444 t
ORIGIN      
        1 atccgcagca gccggcgttg ctggatggcg aatccagtgt atcccagtct gctgtttcac
       61 accccgcagc gtcccaggac gccgcgtttc tggctggcac cacagcggcc ggcgagccga
      121 tggaggaact ggcgatcact gattcagccg gtgccgacgc cgccgcctga ggcccgtcgc
      181 gctttcctgt tccggctctc ctgccactgc ccccgccctg aggggcgcta tactcaaggc
      241 aactcttccc ggagtggacc cttgaactcc cttatcgacc ggatcctgaa ttgggggtgg
      301 cagcccgaac tgcacagcgc catccagatg cgacgcatcc gcacgctgtc cgggtcggcg
      361 ttgctgctgg tgctggtggg cattccgttc tggatgcggg ccatagaatg gcagatcgca
      421 ctgcgcatgg taacggttcc cacggccatc gcgctgacgc tggccggcct ggtgttattg
      481 cgctggcgcc acacggcgcg catgcatttc ctctgcaccc agctgatcag tcttgccctc
      541 tatgttgccg gcatgggcgc catgctcagc agcggtggtc tgggcaatat caccgagggc
      601 tggctgatgc tggtaccggt ggtggccggg atcaaccttg gattgcgtcc tgcgctgcag
      661 tggggggcgg tgatcctgct cacgctggtt gccgtggccc tgctaccgtc cctgggctat
      721 gtgttggtgg accagacgcc accctcccat caagcgtccc agcaggtact gcagggactg
      781 ggcgtttttg tggcgctgat gatcctggtg tcgagttttg tgagccaggt gagccattcc
      841 gaagccgtgc tggcgcagca gaacctgcag ttgcagcagc agataatcga aaaggaaaaa
      901 gccgagcgcg aggcccgcag cgccgaacag gccaagaccc gtttcctggc caacatgagc
      961 cacgagatgc gcacaccgct gaatgcgatt ctgggatttt cacgccggct ggacaaatcc
     1021 ctggcgggcc gtctggacga gcgtgagcat aacgcgttga gccgcatcgc cgagtgcggg
     1081 gagggcatgc tgcggctggt ggacgatctg ttcgatctgt cggccattga tgccggcacg
     1141 ttgatcctgc accggcagcc cgtcgcgctg ggcccgctgc tggcgcgggt cgtggcggag
     1201 cttcagcccc tggcgcaaca gcgcgggctg tccttgcaac agggccaatg tcccgactgc
     1261 tgcgtgaccg gcgacccgga gcggctggag caggtgctga ccagtctgct gcgctttggc
     1321 ctgacatcga cgcaggcggg agatgtgcgt atttcggctc accgccagga ggatgcgcag
     1381 gtctgtgtgt ccatcgagga ctcggcggtg cccctcgatg aagcggcgca actgcggctg
     1441 ctggaccgtt acaaccatct gcacagccgg ggctggcagg atctggacgg gtttgccctg
     1501 ggccccgccc tggcagttga gctgatccgg ctgcacggtg gccgtatcca gattgggccc
     1561 gggtctctgg gtggaaactg tttccgtatt ctgctgccgc cgcatgcgcc ggcgcatgaa
     1621 taataaagtt acgggcgtaa aaaagccccg cgaacggggc gaaggtcgag gtttaacgct
     1681 tgtagaatct gtagtcttcg agtcagcggc ggcgatgctg gataaacgcc cacaccagca
     1741 atcccaaacc aaacagtgac agcgtgcccg gttctggcat ggggccgaaa cgcagggtgc
     1801 cggagccggg aaggatactg gcggtgtcgc cagcagggta gagcagtgga tgggtatcca
     1861 gcgccggaac cgcccggctg acaaaatccg gttccggcgg gtggtccagt gccagcgcaa
     1921 tcagactgtc atccggtgcc gatggtgtga agacgtgcgc cacgggtgcg ttctgtgctg
     1981 acatcgcctg caggttgtcc gccgccatca ggccacactg gccgggcagg ctggtcagtg
     2041 tgcctgccag cgtcagtcct gccaccattg cctttgttgc tgcccgcatg ctgcattcct
     2101 tctgcatatt cagggatgaa caagcaaagc cagggccagc gtcggaaagc catttaaatt
     2161 cagtcagttg tgaactgttt cgcaacaggc ggcgtaacaa ttgtaaagct gtccgacaag
     2221 cagtcagcct cgatacctgt ccgtcaccat ccttagcccc aatcc
//
LOCUS       DASUEG010000946         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1024329, whole genome shotgun sequence.
ACCESSION   DASUEG010000946 DASUEG010000000
VERSION     DASUEG010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024329"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFX11_17810"
     CDS             <1..216
                     /locus_tag="VFX11_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940533.1"
                     /translation="TPLRYRLNTTSADAIGHVTITFHTEGEVRPADHLACAGLLDAIR
                     PAYESAPGVLRHDLEINHVKSDDRLAR"
     gene            complement(252..1562)
                     /locus_tag="VFX11_17815"
     CDS             complement(252..1562)
                     /locus_tag="VFX11_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940534.1"
                     /translation="MHARWLILLLGFLFTANSMAAVLPPPPLERLEAFKVITIEGEDI
                     PAALGSTLKDLSLAAIVDNVMEPVPFQIDQYNEGGGIYFEGWDIPLAGNIKSFDAADK
                     LLFLYKDAGPRRTARDVYDGEVLAEIMLEDATGVARYVYLVKGSRLRSDEQYVRYSSE
                     LGFVETDFYQMRYNPGNHLKWDDFQATDFVGERPLDSMKLRLNMGVVTSLVPLELNND
                     DLVAIPKGEHIGPIRTTTQLDLTMYYLQVPIMHLSMQVHHYPKSVMYDVRGVIPAFRR
                     MLMDKPYLNMSLDANRLLGSTVRMANGPKEPGIVDGRISAIEEQMRKTAFTPDDNWFW
                     VSTLRNLDVVAFLNYLGDFNEPVSPYIQDDLDKADPPEVFLGQLPNLGYTIHSFPKQG
                     FMGLVVSIYFDEGMSGDLEVFTKQLRTAPEIQVRPVRVSTWAPR"
     gene            1808..2689
                     /locus_tag="VFX11_17820"
     CDS             1808..2689
                     /locus_tag="VFX11_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940535.1"
                     /translation="MQAKNLMTLSAMLDRLTTLAAIRELVELYQQPLAAERQFDLRML
                     VPLLEIRLRDIVSAHHALKNQLRSRREQMGNVLQQAEQILACLASMVAVMNSEDNGSK
                     ICIAAQDLMRDFRKVCLDARSMGSAMVDQEMQAIRLNVTLFAETHRIASDILREGLYE
                     EDLIYKQLARITDQLAAACQSPAAGPDIHQNAAHILKMCLDLKPKAELLANQIGKISQ
                     VPLPTVLTEMARQLSGKYTALIGSFDGLLNSLYASDEQQKALAEAAAIYQYIFGKIES
                     ATAGVAAEHSDGADRAK"
BASE COUNT          677 a          828 c          751 g          576 t
ORIGIN      
        1 actccgctgc gctaccgcct caacaccacc agcgccgacg ccattggaca cgtgacgatt
       61 accttccaca ccgaaggcga ggttcgtccc gccgaccatc tggcctgtgc cggcctgctc
      121 gatgcgattc gcccggcgta tgaaagcgcg ccgggcgtgc tgcggcatga cctggagatc
      181 aatcatgtca aatcggatga ccggctggcg cgctgactat cgttgaactg cctgttgatt
      241 gaggcgcctg cttaccgcgg cgcccacgtt gaaacacgca cgggcctgac ctggatctcc
      301 ggcgcggtgc gcagctgctt cgtgaagacc tcaagatcac cactcatgcc ttcatcaaaa
      361 tagatactca ccaccagtcc catgaaaccc tgtttcggaa aactgtggat ggtgtaaccc
      421 agattgggca actggcccag aaacacttcc ggcggatctg ccttgtccag gtcgtcctgg
      481 atgtaagggg aaaccggctc attgaaatcc ccgagataat tcaggaacgc caccacatcc
      541 agattgcgca gcgtgctgac ccagaaccag ttgtcgtcag gcgtaaacgc cgttttgcgc
      601 atctgctctt caatggcact gatgcggcca tccacaatgc cgggttcttt cggcccgttg
      661 gccatgcgca ccgtggagcc cagcaggcga ttggcatcca gcgacatgtt cagatagggc
      721 ttgtccatca acatgcgccg gaacgccgga atcacgccgc gcacatcgta catgacactc
      781 ttgggataat gatgcacctg catgctcaga tgcatgatcg gcacctgaag ataatacatg
      841 gtcaggtcga gctgggtggt ggtgcgaatg ggaccgatgt gttcgccctt cggaatcgcc
      901 accagatcat cattgttcag ttccagcggc accagcgacg tcaccactcc catgttcagc
      961 cgcagcttca tcgaatccag cggccgctct cccacaaaat ccgtcgcctg gaagtcatcc
     1021 catttcaggt gattgccggg gttgtaacgc atctggtaga aatcggtttc cacgaatccg
     1081 agctccgagc tgtaacgcac gtattgttcg tcggagcgca ggcgggagcc tttcaccagg
     1141 tacacatagc gcgcaactcc ggtcgcatcc tccagcatga tctcggccag cacttcgcca
     1201 tcgtagacgt cgcgcgcggt ccggcgcggg cccgcatcct tgtagaggaa cagcagttta
     1261 tcggcggcat caaatgactt gatgttgccc gccagaggga tgtcccagcc ttcaaaataa
     1321 atgccgccgc cttcgttgta ctggtcaatc tggaacggca cgggctccat cacgttgtcg
     1381 acaattgccg ccagggacaa gtctttgagt gttgagccca gcgcagcggg aatgtcctcg
     1441 ccttcgatgg tgatgacctt gaatgcctcc agccgttcca gaggcggcgg tggcagcacg
     1501 gctgccatgc tgttggcggt gaaaagaaaa cccagcaaaa gaatcagcca gcgcgcgtgc
     1561 atttccaaca gccccctgtt tccgtatata aaataatccc catttccgac gatagcggac
     1621 ggattggggg tagggtcaag actcaatgtg caaatcacga cagggaatga caggagatga
     1681 accctcccgc ttgccggaaa catgctatcc acgccatccg acagccgcct tgcgctgaca
     1741 atccattgac ttttaagatg acaccttgac ctgacgctac aggcagcgcc caaacaggaa
     1801 actctccatg caagccaaaa atctcatgac cttgtccgca atgctggatc gcctgaccac
     1861 gctggcagcc atacgggaac tagtcgaact ctaccaacag ccgctggctg ctgaacgtca
     1921 atttgatctg cggatgctgg ttccgttact ggaaatcagg ctcagagata tagtgtccgc
     1981 ccatcacgca ctgaaaaacc agttgagaag caggcgcgag caaatgggaa atgtgctgca
     2041 gcaggcggaa cagattctgg cctgcttggc atccatggtt gcggtaatga atagtgaaga
     2101 caacggcagc aaaatctgca tcgccgccca ggatctgatg cgcgacttca ggaaggtctg
     2161 tctggatgcc cggtcgatgg gttcagcaat ggtcgatcag gaaatgcagg cgatccgtct
     2221 gaatgtcacc ctctttgccg agacgcacag gatcgcaagc gacatattgc gggaaggcct
     2281 ttacgaagaa gacctgattt acaagcagtt ggccagaatc acggaccagc ttgcggcggc
     2341 ttgccaaagt ccagctgccg ggccagacat acaccaaaat gctgcccata tcctgaaaat
     2401 gtgtttagac ctgaaaccca aagccgaatt gctggccaat cagattggga aaatttcaca
     2461 ggtcccgctg ccaacggttc tgacggaaat ggcaaggcag ctgagcggga aatacacggc
     2521 attgattggc agcttcgacg gactgctcaa ttccctgtat gccagtgacg aacagcagaa
     2581 ggcccttgcc gaagccgccg ccatttacca atatattttt ggaaaaattg agagtgccac
     2641 agcgggcgtg gcagcagaac attccgatgg tgccgatagg gcaaaataat aaaggcgcct
     2701 tcgcttactg aacgacgtag cgctgcatta ttcagcccct caattagcgt ttaatccctc
     2761 agcatttgat cgtcggccca cctggcactg atgacgacca tatacacgaa acaagcaatc
     2821 cccctgcaca cc
//
LOCUS       DASUEG010000947         8425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1825613, whole genome shotgun sequence.
ACCESSION   DASUEG010000947 DASUEG010000000
VERSION     DASUEG010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825613"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="yihA"
                     /locus_tag="VFX11_17825"
     CDS             <1..527
                     /gene="yihA"
                     /locus_tag="VFX11_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX4940536.1"
                     /translation="GRSNSGKSSALNTLADQGKLARTSKTPGRTQLINFFRINDEWKL
                     VDLPGYGYAQVPEQLKLEWQKHMGEYLEKRECLAGLFLVMDSRHPLKEFDRMMLDWAQ
                     HHRMRVHILLSKCDKLNRGPAANSLQAVRKEIKPLGDHITAQLFSSHDKTGVDEAREV
                     LANWFGYEDPVAAG"
     gene            complement(922..3672)
                     /gene="polA"
                     /locus_tag="VFX11_17830"
     CDS             complement(922..3672)
                     /gene="polA"
                     /locus_tag="VFX11_17830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX4940537.1"
                     /translation="MSSKPPLILVDGSSYLYRAFHALPPLTTSKGQPTGAIKGVLNML
                     QKLIKDYQPEHMAVVFDAKGKTFRDDLFPEYKSHRPPMPDDLRSQVEPLQNAIRWMGL
                     PLLIIDNVEADDVIGTLCQQASEQGIEVLVSTGDKDMAQLVNDHVTLINTMTDSIMNR
                     DGVIEKFGVAPEQIIDYLTLVGDSVDNIPGVPKCGPKTAVKWLQEYGSLDGVIANADN
                     IKGKVGENLKESLHFLPLSKQLATIRLDVELEQKPQDLKINPPDKEKLFELYKELEFK
                     GWIAELSGQVKSGPAQSGAATADLFSGDATANDPPAPANHDYQCILTEADFNSWLAQL
                     ENCTAFSFDTETTSLNYMEAELVGLSFSIEAGKAAYVPVAHDYPGAPDQLNRDWVLQQ
                     LKPLLEAEKPKKIGQNLKYDKSVLARYDIHIHGIGWDTMLESYVVDSTATRHDMDSLA
                     LKYLGHKTIAFEDIAGKGAKQLTFNQIAIEQASEYAAEDADITLRLHQTLWPQLEGQA
                     SLRTVFEEIEMPLLPVLSRIERTGVLIDTHMLRLQSQQLAKRMLELEQEAYAIAGQPF
                     NLGSPKQLGEILFEKLGIPVLQKTPTGQPSTAEPVLAELALDYPLPKVLMEYRVLSKL
                     KSTYTDKLPELINARTGRVHTSYQQAVAATGRLSSTDPNLQNIPVRTEEGRRIRQAFI
                     APKNCKLLAADYSQIELRIMAHLSGDDSLLNAFKKGLDIHKATAAEVMGVAIDDVTHD
                     MRRNAKAVNFGLIYGMSAFGLAKQLGIGRKEAQEYIDRYFERYPGVHRYMENIRATAA
                     QQGYVETLFGRRLYLPEINSKNVPRRQAAERTAINAPMQGTAADIIKRAMIAVDGWIQ
                     KDQLQSRMIMQVHDELVLEVPEQELPIVRKKLPELMQNAAVLTVPLLVEVGVGDNWDQ
                     AH"
     gene            complement(3679..4068)
                     /locus_tag="VFX11_17835"
     CDS             complement(3679..4068)
                     /locus_tag="VFX11_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4940538.1"
                     /translation="MSKREIISTDKAPKAIGTYSQAVKVGSTVYLSGQIPLDPATMTM
                     VDSSMEASIVRVFENLKAVAEAAGGSLQDVAKLNIFLTDLSHFALVNEVMARYFQQPY
                     PARAAIGVASLPKGAQVEMDAVLELGA"
     gene            complement(4320..6437)
                     /gene="spoT"
                     /locus_tag="VFX11_17840"
     CDS             complement(4320..6437)
                     /gene="spoT"
                     /locus_tag="VFX11_17840"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX4940539.1"
                     /translation="MQTVETLIEKLHYLPPEVAEDVRRAYYFAEQAHEGQYRRSGDPY
                     IVHPLAVSKILADMHMDAQSLQAALLHDVIEDTHVSKEALTDAFGPIVADLVDGVSKI
                     GAIKFESKAEAQAENFRKMVLAMTKDIRVVLVKLADRLHNMRTLGVLHPQKRRRIAIE
                     TLEIYAPIANRLGMNNIRVELEDLCFTHMYPMRAARIKKAIKSARGNRKDMVTKIQDA
                     VTNRIARDGLTARVMGREKHLYSIYNKMKTQRKPFNEIMDVYGFRIVVDSVDMCYRVL
                     GAVHNLYKPVPGRFKDYIAIPKSNGYQSLHTTLIGMNGVPIEIQIRTEDMEAMANNGI
                     AAHWLYKSDPDPAGGAQNRAKEWLKGLLEMQQRAGNSLEFIETVKIDLFPDEVYVFTP
                     KGHILELPSGATPVDFAYAVHTDVGNSCVAARLDHRLVPLSTPIQSGQTIKIITAPGA
                     CPNPAWLSFVTTAKARANIRHYLKNQRRSESVSLGRRLLDKALGSFELNLDAIPEDRI
                     RQALDMSSFKTLDDLLEDIGLGNQMAQIAARRLLPLTQQDTATDDKSKSPPLVIRGAE
                     GMIINLAKCCRPIPGDSVIGHLSSGRGMVIHRDNCKNIATEMKENPEKCLSVEWAADA
                     EGEYSVDLRVQMENERGALAVLASLISQIDANIETINLKEKDAHLSVVSLRITVRNRK
                     HLARAIRRMRTLKAVNKITRMRN"
     gene            complement(6515..6793)
                     /gene="rpoZ"
                     /locus_tag="VFX11_17845"
     CDS             complement(6515..6793)
                     /gene="rpoZ"
                     /locus_tag="VFX11_17845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX4940540.1"
                     /translation="MARVTVEDCLGKVDNRFSLVLVASKRARQLATGGKDPYVQWEND
                     KPTVVALREIAEGYVDQSILTRKPEPKEYKEPVSFRHAFAAIQDDDDE"
     gene            complement(6861..7472)
                     /gene="gmk"
                     /locus_tag="VFX11_17850"
     CDS             complement(6861..7472)
                     /gene="gmk"
                     /locus_tag="VFX11_17850"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX4940541.1"
                     /translation="MQGTLFVVSAPSGAGKTSLVKALVESTPNIHVSVSHTTRPQRPG
                     EVNGVNYHFVDKDAFGAMLNDGAFLEQAEVFGNLYGTSKYTVADKLKAGVDVVLEIDW
                     QGAQQIRRQAPEAVSIFILPPSRLVLEQRLLGRGQDPVEVIRKRMAQATEQISHHVEF
                     DYLIINDDFEKALKEMQAIVLCQRLGILKQCERHSTLIKSLLS"
     gene            complement(7533..>8425)
                     /locus_tag="VFX11_17855"
     CDS             complement(7533..>8425)
                     /locus_tag="VFX11_17855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX4940542.1"
                     /translation="PPPRRSLPMIRSMTAFSRKEMKAPWGTLSWELRSVNHRYLEISP
                     RLPDTLRDLENPVREKLRQSLDRGKVECTLRLKAENIVPGKIDINEQFVRQLLEAAHR
                     ISALTGEAASLSATTLLNWPGVVQTPETDQSEIQQTALALFESTLEDFVTSREREGSQ
                     LQQIILERLDKIDDQVAIVRGQLPAILQAQKDKLLARLAEINANPDKDRLEQELVYLA
                     QKADVDEELDRLITHVREVRRSLKQGGACGRRLDFLMQELNREANTLGSKSVHTLTTQ
                     VAVELKVLIEQMREQIQNIE"
BASE COUNT         1516 a         2340 c         2642 g         1927 t
ORIGIN      
        1 ccggccgctc caactccggc aagtccagtg ccctcaatac cctggcggac cagggcaaac
       61 tggcccgcac cagcaaaacg cccggtcgca cccagctgat caactttttc cgtatcaacg
      121 acgagtggaa attggtggac ctgccgggct atggctacgc ccaggtgccc gagcagctga
      181 aactggagtg gcagaaacac atgggggaat acctggaaaa gcgcgagtgc ctggcggggc
      241 tgttcctggt gatggattcc cgtcatccgc tgaaggagtt cgaccgcatg atgctggact
      301 gggcccagca ccaccgcatg cgggtgcata tcctgctgag caagtgcgac aagctcaatc
      361 gtggccctgc cgccaattcc ctgcaggcgg tgcgcaagga gatcaagccc ctgggcgacc
      421 atatcaccgc ccaactgttt tcttcccacg acaaaaccgg ggtggacgag gcgcgtgagg
      481 tgttggccaa ctggttcggt tacgaggatc cggtcgccgc tggttgaaat ccagcggaat
      541 tgcgcaaagc ccgcgcgatg cagatcggcg ggcttttttg tggccggaag tttgggcgcc
      601 ctattggaaa agatgtcggg ataaagtgct gaaagcaggg ggagccaaat cgcaggcaaa
      661 aaaagaccgg tagtcttggg gcgaactacc ggtcagggtc cggctcttgg gggaaagccg
      721 gtttgctgta gccgaagaat cggctgattg gggattgctg ccttagcttc atgaattgag
      781 acggccggtg ctggaaaaag ttcaccaaga gtggaaaatt ttttcctttt ttaggccgtt
      841 gaggttcggc agacgatcct tcatcttttt ttctggttat ataagcccac cggtaccggc
      901 aacattgggg gattgcctaa cctaatgagc ttgatcccaa ttatccccta ctccaacttc
      961 aacaagcaag ggcaccgtta acacggcggc gttttgcatc agttccggca attttttccg
     1021 cacaatgggc aattcctgct caggtacttc cagtaccagt tcgtcgtgca cctgcatgat
     1081 catgcgggat tgcagctgat ctttttgtat ccagccatcc acggcgatca tggcgcgctt
     1141 gataatgtca gccgcggtgc cttgcatggg cgcgttgatt gcggtgcgtt ccgccgcctg
     1201 acggcgcggc acgtttttgg aattgatttc cggcagatac agacgacggc caaacagcgt
     1261 ttccacatag ccctgctgcg cggcggtggc gcggatattt tccatgtagc gatgcacgcc
     1321 gggataacgc tcgaaataac gatcgatata ttcctgcgct tccttgcgac cgatgcccag
     1381 ctgtttcgcc agaccaaacg ccgacatgcc gtagatcaaa ccgaaattca ctgctttcgc
     1441 gttgcggcgc atgtcgtggg tgacgtcgtc gatggcaacg cccatcactt ccgccgccgt
     1501 ggccttgtgg atgtcgagcc cctttttgaa tgcattgagc aggctgtcgt cgccggacag
     1561 atgcgccatg atgcgcagtt cgatctggga ataatccgcc gccagcagct tgcagttttt
     1621 cggcgcgatg aaagcctggc ggatgcggcg gccttcctcc gtgcgcacgg gaatattctg
     1681 caggttggga tcggtggaag acaggcgtcc cgttgccgcc accgcctgct gataggacgt
     1741 gtgcacgcgc ccggtgcggg cattgatcag ctcgggtaat ttatcggtat aggtggattt
     1801 cagtttgctg aggacgcgat attccatcag cactttcggc agcgggtaat ccagcgccag
     1861 ttcggccagc accggctctg cggttgaagg ttgtccggtc ggtgttttct gcaacacggg
     1921 aatgccgagt ttttcaaaca ggatttcacc gagctgtttg ggcgagccga gattgaacgg
     1981 ctgaccggcg attgcatagg cttcctgttc cagttccagc atgcgcttgg ccagctgctg
     2041 gctttgcagg cgcagcatgt gggtgtcgat caacacgccg gtgcgttcga tgcgcgacaa
     2101 cactggcaac agcggcattt cgatttcttc gaacactgtg cgcaatgatg cctgaccttc
     2161 cagttgcggc cacagggtct gatgcagccg cagcgtgatg tcggcatctt ccgccgcata
     2221 ttcgctggcc tgttcgatgg cgatctggtt gaaggtgagc tgcttggcgc ccttgccggc
     2281 aatgtcttca aacgcaatgg ttttgtgacc caggtatttc agcgccaggc tgtccatgtc
     2341 gtggcgggtg gcggtggaat cgacgacgta ggattccagc atggtgtccc agccgatgcc
     2401 atggatatga atgtcgtagc gcgccagcac gctcttgtcg tacttcaggt tctgaccgat
     2461 tttttttggc ttttctgctt ccagcaaggg cttgagctgt tgcagtaccc agtcgcgatt
     2521 caattgatcc ggcgcgccgg gataatcatg cgctaccggc acataggctg ccttgcccgc
     2581 ttcaatgctg aacgacagcc ccacgagttc ggcttccatg taattcaggc tggtggtttc
     2641 ggtgtcgaag gaaaacgcgg tgcagttttc taattgtgcc agccaggaat tgaaatccgc
     2701 ttcggtgagg atgcattgat agtcatggtt ggcaggcgca ggtggatcgt tggcggtggc
     2761 gtcgccggaa aacagatcgg cggtagccgc gcctgattgt gctgggcctg atttcacctg
     2821 ccccgacaat tccgcaatcc agcccttgaa ttccagttct ttgtacagct cgaacagttt
     2881 ttccttgtct ggcggattga ttttcagatc ctgcggtttt tgctccagtt cgacatccag
     2941 ccggatggtg gcgagctgtt ttgacagcgg cagaaaatgc aggctttcct tcaggttttc
     3001 gcccaccttg cccttgatgt tgtcggcgtt ggcgatcacg ccatccagcg aaccgtattc
     3061 ctgcagccat ttcaccgcgg ttttcggccc gcatttcggt acgccgggaa tgttgtcgac
     3121 gctgtcgccc accagggtca ggtagtcgat gatctgctcc ggcgccacgc cgaatttttc
     3181 gatcacgccg tcgcggttca tgatggaatc ggtcatggtg ttgatcaggg tgacgtgatc
     3241 attcaccagc tgcgccatgt ccttgtcgcc ggtggagacg agcacttcga taccctgttc
     3301 gctcgcctgc tggcacaggg tgccgatgac atcatccgct tcaacgttgt cgatgatcag
     3361 cagcggcagg cccatccagc ggatcgcgtt ctgcagcggt tccacctgcg agcgcagatc
     3421 gtccggcatc ggcggacggt gggatttgta ctccggaaac agatcgtcgc gaaaggtttt
     3481 gcctttggca tcgaacacca cggccatgtg ctcgggctgg taatccttga tcagcttttg
     3541 cagcatgttg agcacgccct tgatggcgcc ggtgggctgg cctttcgacg tggtgagcgg
     3601 cggcagcgca tgaaaggccc ggtacagata ggaggaaccg tcgacgagta tcaggggagg
     3661 tttggaggac atggcggttc aggcacccag ttccagcacc gcgtccattt ccacctgcgc
     3721 gcctttgggc agcgaggcaa cgccgatggc cgcccgcgcc ggatatggct gctggaaata
     3781 acgcgccatc acttcattca ccaatgcaaa atgactcaga tcggtgagaa aaatattcag
     3841 cttggccaca tcctgcagcg agccaccggc ggcttctgcc accgctttca gattttcgaa
     3901 cacgcgcacg atgctcgctt ccatgctgga atccaccatg gtcatggtcg ccgggtccag
     3961 cggaatctgg ccggacagat agacggtgct gccgactttt acggcctggg aataggtacc
     4021 gatggccttg ggtgccttgt cggtggaaat gatttcgcgc ttgctcatga acggctcctt
     4081 agtggtcagg atgatgcaga aacttcaacg aaaaataatc agaagatcag gcgatgggca
     4141 gttccgccat tttgcgcagc cgggcatcgc agaccaaacc ggaaaaaggt ggcgagtgtc
     4201 tgagcgcgta acgcaagttg cgccaccgcc ggattgggcg agaagcacag ggtagccgaa
     4261 ggccaagcgg catggcggaa ctgcccatcg cctgatcttc gccacgcgac aggatgctat
     4321 cagtttctca tccgcgtgat cttgttcacc gctttcagcg tacgcatgcg gcggatggcg
     4381 cgcgccaggt gtttgcggtt gcgcacggtg atgcgcaggc tcaccacaga cagatgggca
     4441 tccttttcct tcagattgat ggtttcgata ttggcatcga tctgcgagat cagcgacgcc
     4501 agcaccgcca gcgcgccacg ttcgttttcc atctgcaccc gcaggtccac agaatattca
     4561 ccctcggcat cggcggccca ttccaccgac aggcatttct ccggattttc cttcatttcc
     4621 gtggcgatgt tcttgcagtt gtcgcggtgg atcaccatgc cgcgaccgga actcagatgc
     4681 cctatcacgg aatcgccggg aatcggccgg caacatttgg ccagattgat gatcatgcct
     4741 tcggcaccgc ggatcaccag cggcggcgat ttggacttgt cgtcggtggc ggtgtcctgc
     4801 tgggtcagtg gcagcagacg gcgcgcggca atctgcgcca tctggttgcc caggccgatg
     4861 tcttccagca gatcgtcgag agtcttgaag ctggacatgt ccagcgcctg gcggatgcgg
     4921 tcttccggaa tggcatccag attcagttcg aatgagccca gcgccttgtc cagcagacgg
     4981 cggcccagcg aaacggattc ggagcggcgc tggtttttca gatagtggcg gatgttggcg
     5041 cgggctttcg cggtggtgac gaagctgagc caggccgggt tggggcaggc gccaggggcg
     5101 gtgatgatct tgatggtctg gccgctctgg attggcgtgc tcaatggcac cagacggtga
     5161 tccagccgcg ccgccacgca gctgttaccc acatcggtgt gcacggcata ggcaaaatcc
     5221 accggcgtgg cgcccgatgg caattccaga atgtgacctt tgggcgtaaa gacatagacc
     5281 tcgtccggga acagatcgat cttcacggtt tcaatgaatt ccagcgagtt gccggcgcgc
     5341 tgctgcattt ccagcagacc tttcagccat tccttggcgc gattctgcgc gccgcccgcc
     5401 ggatcgggat cggatttata cagccagtgc gccgcgatgc cgttgttggc catcgcctcc
     5461 atgtcttcgg tgcggatctg gatttcgatc ggcacgccgt tcatgccgat cagcgtggta
     5521 tgcagcgact gatagccgtt ggacttggga atcgcgatgt aatccttgaa ccggcccggc
     5581 accggtttat atagattgtg cacggcgccc agcacgcgat aacacatgtc caccgaatcg
     5641 acgacgatgc ggaaaccgta cacgtccatg atctcattga acggtttgcg ctgggttttc
     5701 atcttgttgt agatgctgta caggtgtttt tcgcggccca tcacgcgggc ggtgaggccg
     5761 tcgcgggcga tgcggttggt aacggcatcc tggatctttg tcaccatgtc cttgcggttg
     5821 ccgcgggcgg acttgatggc ttttttgatg cgggcggcac gcatggggta catgtgggtg
     5881 aagcagagat cttccagttc cacccggatg ttgttcatgc ccagccggtt ggcgatcggc
     5941 gcgtagattt ccagcgtttc gatcgcaatg cggcggcgct tctgcggatg cagcacgccc
     6001 agggtgcgca tgttgtgcag gcggtccgcc agcttcacca gcaccacgcg gatgtccttg
     6061 gtcatcgcca gcaccatctt gcggaagttt tccgcctggg cctcggcctt ggattcgaac
     6121 ttgatggcgc cgatcttgga cacaccgtcc accagatcgg cgacgatggg gccgaaagcg
     6181 tcggtgaggg cttccttgga aacgtgggta tcctcgatca cgtcatgcag cagcgcggcc
     6241 tgcaggctct gcgcatccat gtgcatgtcg gcgaggatct tggaaacagc caggggatga
     6301 acaatatagg ggtcgccgct gcggcggtac tggccttcat gggcctgctc ggcgaaatag
     6361 taggcgcgac gcacgtcctc tgccacttcc ggcggcagat agtgaagttt ttctataagg
     6421 gtttcaactg tttgcacgtg cccaatccct cagtgtcgtt caagtgtaaa gggattgggc
     6481 atgggagaga aggcgggaaa aagcggggcc ggacttattc gtcgtcgtcg tcctggatgg
     6541 cggcgaaggc gtgacggaag gagaccggtt ccttgtattc cttgggctcg ggcttgcggg
     6601 tcaggatgga ctgatccaca tagccttcgg cgatttcgcg cagggcaacc acggtcggtt
     6661 tgtcgttttc ccactgcacg tagggatctt tgccgccggt ggccagttgg cgcgcgcgct
     6721 tggacgcgac cagcaccagc gagaatcggt tgtctacttt acccagacag tcttcaacgg
     6781 taacccgtgc cattgtcgta cctcggttgg aatttcaggc aagctggcta gtttactggt
     6841 tttcccggcc ctgacaagcc ttatgacagg agcgatttga tcagcgtgga atgccgttcg
     6901 cattgcttga gaatgcccag gcgctggcac agcacaatgg cctgcatttc cttgagggct
     6961 ttctcgaaat cgtcgttgat gatcaggtag tcgaattcca catggtggga gatctgctcg
     7021 gtggcctggg ccatgcgctt gcggatgacc tccaccggat cctggccgcg gccgagcagg
     7081 cgctgttcca gcaccagtcg cgacggcggc aggatgaaga tgctgacggc ctccggagcc
     7141 tggcggcgga tctgctgggc gccctgccag tcgatttcca gcaccacgtc cacgccagcc
     7201 ttgagcttgt ccgccactgt gtacttgctg gtgccgtaca ggttgccgaa cacctcggcc
     7261 tgttccagga aggcgccgtc gttgagcatg gcgccgaagg catccttgtc cacgaaatgg
     7321 tagttgacgc cgttcacttc gcctggccgc tggggccggg tggtgtggga cacggacacg
     7381 tggatgttgg gggtggattc caccagggcc ttgaccagac tggtcttgcc ggcgccggag
     7441 ggggcggaga cgacaaacag ggtgccttgc atgcggggcc tttcgggtaa gtcgtgtgga
     7501 tgggcgtatc gtttgagtat agctgctgcc ggctactcga tattctggat ctgttcccgc
     7561 atctgctcga tgaggacttt cagttccacc gccacctggg tggtgagggt gtggaccgac
     7621 ttggagccca gggtgttggc ctcgcggttg agttcctgca tcaggaaatc gagccggcgg
     7681 ccgcaggcac ccccctgttt caggctgcgc cgcacttcgc ggacgtgggt gatgaggcgg
     7741 tccagttcct cgtcgacgtc ggctttctgg gccaggtaga ccagttcctg ttccagccgg
     7801 tccttgtcgg gattggcgtt gatttcggcc aggcgggcca gcagcttgtc tttctgtgcc
     7861 tgcaggatgg cgggcagctg gccacgcacg atggcgacct ggtcgtcgat cttgtccagc
     7921 cgttccagga tgatctgctg cagctggctg ccttcgcgtt cgcggctggt gacgaagtct
     7981 tccagagtgg attcgaacag ggcgagggcg gtctgctgga tttcggattg gtcggtttcc
     8041 ggggtctgga ccacgccggg ccagttgagc agggtggtgg cggacaggct ggcggcttca
     8101 ccggtgaggg cgctgatgcg gtgggcggct tccagcagct gtctgacgaa ctgttcgttg
     8161 atgtcgatct tgccgggcac gatgttctcg gccttgaggc gcagggtaca ttccaccttg
     8221 ccgcggtcga ggctctggcg cagtttttcc cgcaccgggt tttccaggtc gcgcagggtg
     8281 tcgggcaggc gcggcgagat ttccaggtag cggtggttta ccgagcgcag ttcccaggac
     8341 agggttcccc agggggcttt catttccttg cggctgaagg cggtcatgct gcggatcatg
     8401 ggcaggctcc tgcgcggggg tgggt
//
LOCUS       DASUEG010000948         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_455839, whole genome shotgun sequence.
ACCESSION   DASUEG010000948 DASUEG010000000
VERSION     DASUEG010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455839"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFX11_17860"
     CDS             <1..482
                     /locus_tag="VFX11_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4940543.1"
                     /translation="KALSNDPDFSQARNNYAAYLFKRGDYAQAIEHLERVTKDYRYEK
                     RYTAYENLGLCYLRTGDSDKALASFQRALKLNNSLPISTLEIAQIKFDRGENAVALEY
                     LTRHEKLSKPSPKQLWLGIRLQRILGDQNKLGSYELQLRNMFPASPEYQAYKASLSS"
     gene            479..1564
                     /locus_tag="VFX11_17865"
     CDS             479..1564
                     /locus_tag="VFX11_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX4940544.1"
                     /translation="MTASPGEPVSTTSSSDNGYDKAPAVSPGAQLQKARKERNLEIPA
                     VAEQLNLSPGVVRALEADDYRMLPNATFVKGYLRSYARVLGIAGDDLVRAYETLTGRN
                     KPVVVEPVAPPRIRKSGNSPKYAIIALLVAGLLGLVLWLMPGEPERTEPPMAIVTDPA
                     ALASADDGDLDDSAAVSADSAADKPALADASQHAAGEDGSSADAGSRDSESGFSLVTP
                     EAAVTATGNPVSAPETPETLPGPSVAAPEIVVPAAPVAAPESSPVASEPVQAEPASTV
                     PPSGRGMLKLAFSGDCWVEVRDVRGKMVFSSLKRAGDDVTLNVAVPVSVKLGNGDVVA
                     VTFNGEPVAFSTTASRKVIRLTLGESD"
     gene            1596..2732
                     /gene="ispG"
                     /locus_tag="VFX11_17870"
                     /gene_synonym="gcpE"
     CDS             1596..2732
                     /gene="ispG"
                     /locus_tag="VFX11_17870"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX4940545.1"
                     /translation="MHCESPIKRRKSRQIMVGKVPVGGDAPITVQSMTNTETCDVEAT
                     VGQIRRMEDAGVDIVRVSVPSLEAADAFGEIRKRVNVPLVADIHFDYKIALAVAEKGV
                     DCLRINPGNIGREDRIRAVVDAALDKNIPIRVGVNAGSLEKELQKKYGEPTPEALVES
                     AMRHVDILDRMNFHNFKLSLKASNVFMTVAAYRLIAAQIDNPLHLGVTEAGGLRSGTV
                     KSAIALGALLMEGIGDTLRISLAADPVEEVKVGFDILKSLGLRTKGINLIACPSCSRQ
                     NFDVISTVNALEARLEDVKMPLDVAIIGCIVNGPGEAKEVDIGLTGGTPANLIYRDGK
                     PSHKVSPEELVDELERVIRARVAEVEANPEAAPEKLRPKITLGV"
     gene            2798..>4050
                     /gene="hisS"
                     /locus_tag="VFX11_17875"
     CDS             2798..>4050
                     /gene="hisS"
                     /locus_tag="VFX11_17875"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX4940546.1"
                     /translation="MTQRIKAIRGMNDILPQDSPVWQFLESRVRQLLSQYGYGEIRLP
                     IVEYTDLFKRSIGEVTDIVEKEMYTFEDRNGESLTLRPEGTASCVRAGEEHGLLYNQV
                     QRLWYTGPMFRYEKPQKGRYRQFHQIGVETFGMGTPDIDAELIIMTARLWRELGISDR
                     VTLQLNSLGSNAARAEFRAALVAFLQEHREQLDEDSKRRLESNPLRVLDSKDHGTQAV
                     LKNAPSLLDFLDAESRDHFEQLKAMLTAAGVKFDINPRLVRGLDYYCKTVFEWVTSAL
                     GAQGTVCAGGRYDGLVEQLGGKATPAVGFAMGMERLVLLLDTAGVVQHLTPAPDVYVV
                     SEDATAALLLAESLRDQAPSLKVQCHCGGGKFKKQLKKADDIGARFALILGESEISSG
                     QVGIKFLREAREQITLSQTQLHTFFA"
BASE COUNT          831 a         1205 c         1264 g          750 t
ORIGIN      
        1 agaaagcctt atcgaacgat ccggattttt cccaggcacg caacaactac gcagcctatc
       61 tgttcaagcg cggcgattat gcccaggcca ttgagcatct ggagcgcgtc accaaggact
      121 accgttatga aaagcgctac accgcctatg aaaatcttgg cctgtgctac cttcggaccg
      181 gtgacagcga caaggcgctg gcgtcgttcc agcgggcgct gaaactgaat aacagcctgc
      241 caatttccac attggaaatt gcccagatca agtttgaccg cggtgaaaac gcggtcgcgc
      301 tggagtacct gacgcggcat gagaagttgt ccaaacccag ccccaaacag ttgtggctcg
      361 gcattcgctt gcagcgtata ttaggcgacc aaaacaagct gggcagttac gagttgcagc
      421 tgcgcaacat gttccccgct tctcccgaat atcaggctta taaggcatca ctcagctcat
      481 gaccgcgtca cccggcgaac ctgtttccac cacctcctcg tcggacaatg gttacgacaa
      541 ggcgccggcc gtttcgccag gcgcgcagct gcagaaagcc cgcaaggaac gcaaccttga
      601 aattcccgcg gtggccgagc agctgaacct gtcgcccgga gtggtgcggg cgctggaagc
      661 ggatgactac cggatgctgc ccaacgcgac gttcgtgaaa ggctatctgc gcagttacgc
      721 gcgagtgctg ggcattgccg gtgatgactt ggtgcgggcc tatgaaaccc tgaccggtcg
      781 caacaaaccg gttgtggtcg agccggtggc gccgccccgg attcgcaaat ccggtaacag
      841 tcccaagtat gcgatcattg cattgctggt ggcgggcctg ctgggcctgg tgctgtggtt
      901 gatgcccggc gagccggagc gaaccgaacc ccccatggcc atcgtgaccg atcctgctgc
      961 gctggccagt gccgatgacg gcgatctgga tgacagcgcg gctgtcagtg ccgattctgc
     1021 agcagacaag cccgctttgg ccgacgcaag tcagcacgcg gcaggtgaag atggcagtag
     1081 cgcggacgcc ggaagccgag atagtgaaag cggtttttcg ctggtgacgc cggaagcagc
     1141 cgtgacggct accgggaatc ctgtctccgc accggagacg ccggaaacgc tccctggtcc
     1201 gtcggttgca gcgccggaga ttgtcgtgcc tgccgctccg gttgcagcgc cggaatccag
     1261 cccggtcgcg tccgagccgg tccaggctga accagcgtcg actgttcccc cgtcgggtcg
     1321 gggcatgctc aagctggctt tcagtggcga ttgctgggtt gaagtgcgcg atgtgcgtgg
     1381 caagatggtt ttctcgtccc tcaagcgggc cggggatgat gtgacgctga atgtggcggt
     1441 acccgtcagt gtcaaactgg gcaatggaga tgttgttgcc gtaacgttca atggcgagcc
     1501 ggtggcgttt tccacgactg ccagccgcaa ggtgatccgt ctgacgctgg gcgaatccga
     1561 ctgatcgttt tccacctttt ggcaggagtg ccaatatgca ttgcgaatcc cccatcaagc
     1621 gccgcaaatc ccgtcagatc atggttggca aggtgccggt cggtggtgat gcacccatca
     1681 ccgtgcagag catgaccaac actgaaacct gcgacgtcga ggctaccgtg ggtcagatcc
     1741 gccgcatgga agatgcaggg gtcgatatcg tgcgggtatc ggtgccgtca ttggaagcgg
     1801 cggatgcatt cggcgaaatc aggaaacggg tcaatgtgcc gctggtggcg gacattcact
     1861 ttgactacaa gatcgcgctg gcagtggccg agaagggtgt ggactgcctg cgcatcaatc
     1921 cgggtaacat cggtcgcgag gaccgcatcc gtgccgtggt ggatgcagcc ctcgacaaga
     1981 acatcccgat ccgggtgggc gtgaacgccg gctcactgga aaaggaattg cagaagaagt
     2041 acggcgaacc gacgccggaa gcgctggtgg aatccgccat gcgccacgtc gatattctcg
     2101 accggatgaa tttccacaac ttcaagctca gcctgaaggc atccaacgtg ttcatgacgg
     2161 tggcagccta ccgtctgatc gcggcccaga tcgacaatcc gctgcacctg ggtgtgaccg
     2221 aagccggcgg cctgcgctct ggcaccgtga aatccgccat tgccctgggg gcgctgctga
     2281 tggaaggcat cggcgacacc ctgcgcatat ccctggcggc ggacccggtt gaagaagtga
     2341 aggtgggctt cgacatcctg aaaagcctgg gtctgcgcac caagggcatc aacctgatcg
     2401 cctgtcccag ctgctcgcgc cagaacttcg atgtgatttc caccgtgaat gccctggaag
     2461 cccggctgga agatgtgaag atgccgctgg atgtggccat cattggctgt atcgtcaatg
     2521 gtcctggcga agccaaggaa gtggacatcg gcctcaccgg tggcaccccc gccaacctga
     2581 tctaccgcga cggcaaaccc agccacaagg tgtcgccgga agagctggtg gacgagctgg
     2641 aacgggtgat ccgcgcccgg gtggcggaag tggaagccaa ccccgaagcc gcccccgaaa
     2701 agctccggcc aaagataaca ctgggcgtct gatctggctt ataatcgggc gccttgccga
     2761 ggggcatgcg cccgtaacag cctaaggatt caccgttttg acccagcgta tcaaagccat
     2821 tcgcggcatg aatgacatcc tgccgcagga ttcccccgtc tggcagtttc tggaaagccg
     2881 cgtgcgccag ttgctgagcc agtacggcta tggcgaaatc cgcctgccga tcgtcgaata
     2941 caccgacctg ttcaagcgtt ccatcggcga agtcaccgac atcgtcgaaa aggaaatgta
     3001 cacctttgag gaccgcaacg gcgaaagcct gaccctgcgc ccggaaggca ccgccagctg
     3061 cgtgcgggcc ggggaagagc acggcctgct gtacaaccag gtgcagcgcc tgtggtacac
     3121 cggccccatg ttccgctatg aaaaacccca gaaaggccgt taccgccagt tccaccagat
     3181 cggggtggaa accttcggca tgggcacgcc ggatatcgac gccgaactca tcatcatgac
     3241 tgcgcgcctg tggcgcgagc tgggcatatc cgatcgggtg accctgcagc tgaacagcct
     3301 gggttccaat gctgcacgcg ccgaattccg tgcggcactg gtggccttcc tgcaggaaca
     3361 ccgcgaacag ctggacgagg acagcaagcg ccgtctggaa tccaatccgc tgcgggtgct
     3421 ggacagcaag gatcacggca cccaggccgt tttgaagaac gcccccagcc tgctggattt
     3481 tctggacgcc gaatcccgcg atcatttcga acagctcaaa gccatgctga cagcggcggg
     3541 agtgaaattc gacatcaatc cccgcctggt gcgcggcctg gattactact gcaagacggt
     3601 gttcgagtgg gtgaccagcg cgctgggtgc ccagggcacg gtctgcgccg gcggccgcta
     3661 cgatggtctg gtggaacagc tgggtggcaa ggccacgccc gccgtcggtt tcgccatggg
     3721 catggagcgc ctggtgttgc tgctggatac tgccggcgtg gtgcagcacc tgacgccggc
     3781 accagatgtt tacgtggtgt cggaagatgc cacggccgca ttgctgctgg cggagtccct
     3841 gcgcgatcag gcgccgtcgc tgaaagtgca gtgccattgc ggtggcggca aattcaagaa
     3901 gcagttgaaa aaggccgacg acatcggcgc ccgttttgcc ctgatcctgg gtgaatccga
     3961 gatcagcagc ggccaggtcg gcatcaagtt tctgcgcgag gcgcgggaac aaatcacgct
     4021 ttcccagact caacttcaca ccttttttgc
//
LOCUS       DASUEG010000949         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2171130, whole genome shotgun sequence.
ACCESSION   DASUEG010000949 DASUEG010000000
VERSION     DASUEG010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171130"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFX11_17880"
     CDS             complement(<1..1005)
                     /locus_tag="VFX11_17880"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HEX4940547.1"
                     /translation="MFKEWNLELNQFLILLAIGAFGGWATGYPGWSIALVSVTYSFWM
                     LFRARDLVRWLRSGSDADAPEGSGLWGALYDHLFQQQKQQRQQINHLHSIIARAQQST
                     NAIRDAVIVVDRYGNLEWWNEAGSHWLGLKTSTDQGQPITNLLRDPRFVRFFNRGQFD
                     STLEIPAVLHPGVVLQYQVTTFGDGERLLVVRDVTRLHNLEQMRKDFVANVSHELRTP
                     LTVIKGYLETFLDVMGENNPQLKRGLGQMQLQAQRMEMLVNDLLLLSRLETENTTQPM
                     KPVQVGRLLKQVFGDAQALNGDKRHDIRLDLDENLAIFGDENELRSAFSNLVVNAVKY
                     T"
     gene            complement(1087..1776)
                     /gene="phoB"
                     /locus_tag="VFX11_17885"
     CDS             complement(1087..1776)
                     /gene="phoB"
                     /locus_tag="VFX11_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX4940548.1"
                     /translation="MPETKVLIVDDEEAIREMISVALELAGFQWIEAENAQVAHSRIV
                     DERPDLILLDWMLPGVSGIELARRLKRDEATGEIPIIMLTAKSEEDNKIQGLDAGADD
                     YITKPFSTRELISRINAVLRRAQGDRSKSILVSGLALDPISHRITADERPVEMGPTEY
                     RLLEFFMTHQDRAYSRAQLLDQVWGGNVYVEDRTVDVHIRRLRKALAPYKYDHLVQTV
                     RGTGYRFSTKI"
     gene            complement(1935..2819)
                     /gene="ubiA"
                     /locus_tag="VFX11_17890"
     CDS             complement(1935..2819)
                     /gene="ubiA"
                     /locus_tag="VFX11_17890"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX4940549.1"
                     /translation="MKELQLSNSPLRRLPDFIQLGRLDRPIGIYLLLWPALWALWIAA
                     EGKPSFKLIFIFVMGVILTRTGGCVINDYADRDFDGQVQRTANRPLATGRISGKEALA
                     VAALIGLLAFVLVLFTNLFTIVLSVGGLLLACTYPYMKRHTYLPQVVLGAAFAWSIPM
                     AFAAVGESVPLHAWLIYMATLSWTMAYDTLYAMVDRDDDIQAGIKSTAILFGDADLMM
                     VGILNGLAMFALWLLGNQLQFSLWYYAGLLAAVALWTWQLWQARTRDRDACFKAFLHN
                     HWVGAAIFAGLFLHYSLN"
BASE COUNT          586 a          874 c          942 g          574 t
ORIGIN      
        1 cgtgtacttc accgcattca ctaccagatt ggaaaacgcg ctgcgcaatt cgttttcatc
       61 gccaaaaatc gccaggtttt catccaggtc cagccggatg tcgtggcgtt tgtcgccatt
      121 cagtgcctgg gcatcgccga atacctgctt cagcaagcgg cccacctgca ccggcttcat
      181 gggctgcgtg gtgttttcgg tttccagtcg tgacagcagc agcagatcgt tcaccagcat
      241 ttccatgcgc tgcgcctgca gttgcatctg ccccaggccg cgtttcagct gcggattgtt
      301 ttcgcccatc acatcgagaa aggtttccag atagcctttg atcaccgtca gcggcgtgcg
      361 cagttcatgc gacacgttgg cgacgaaatc cttgcgcatc tgttccaggt tgtgcagacg
      421 ggtgacatcg cgcaccacca gcagccgctc gccatcacca aaggtggtga cctgatattg
      481 cagtacgacg cctggatgca ataccgcggg aatttccagg gtgctgtcga actggccgcg
      541 attgaaaaaa cgcacgaagc ggggatcgcg cagcaggttg gtgatgggtt ggccctggtc
      601 ggtgctggtt ttcaggccca gccagtggct gccggcttcg ttccaccatt ccagattgcc
      661 gtagcggtcg accacgataa cggcatcgcg aatggcgttg gtggattgct gtgcgcgggc
      721 gatgatggag tgtaggtggt tgatctgctg ccgctgctgt ttttgttgct ggaacagatg
      781 atcgtaaagc gcgccccaca atccgctgcc ttcgggggcg tcggcgtcgc taccgctgcg
      841 cagccagcgc accagatcgc gcgcacggaa cagcatccag aacgaatagg tcacactgac
      901 cagcgcgatg ctccagccgg gataaccggt ggcccagccg ccgaacgcgc caatggcgag
      961 caggatcaga aactggttca gttcaaggtt ccactcttta aacacgcaac actcacctgt
     1021 gaagggcggg gttcaatcat agcccagccg ccgttccagt acattgagtg acagcaacaa
     1081 cgccgctcag atcttggtgg aaaaccggta gccggtgccg cgcacggtct gcaccagatg
     1141 atcgtatttg tacggagcca gtgccttgcg cagacggcgg atgtgcacgt ccacggtgcg
     1201 gtcttccaca tacacgttgc caccccagac ctgatccagc aactgggcgc gggaataggc
     1261 gcggtcctgg tgggtcatga agaattccag cagccggtac tcggtggggc ccatttccac
     1321 cggtcgctcg tcggcggtga tgcggtggct gatcggatcc agcgccaggc cgctgaccag
     1381 gatcgacttg ctgcggtcgc cctgggcgcg ccgcaatacg gcgttgatgc gggaaatcag
     1441 ttcgcgggtg gagaagggct tggtgatgta atcgtcggcg ccggcgtcga ggccctggat
     1501 cttgttgtcc tcttcacttt tggccgtgag catgatgatg ggaatctcgc cggtggcttc
     1561 gtcgcgcttc agacggcggg ccagttcgat accgctcaca cccggcagca tccagtccag
     1621 caggatcagg tcggggcgct cgtccacaat ccggctgtgg gccacttgcg cgttctccgc
     1681 ctcgatccat tggaatccgg ccagctccag ggccaccgag atcatctcgc ggatggcttc
     1741 ctcatcgtca actatcaaaa ctttggtttc aggcatggca aaaaggttcc ggttccccaa
     1801 atcagcgtgc cctatttaat gccgctaatg ttacagcaat atgacgaaaa tgggtcaacg
     1861 gcggcggaaa cggccgggat ttggcctgga acgggtaagt tgcgtgccgg atgaaatggc
     1921 gctgccagcg ctggttagtt gagggagtag tgcaggaaca aacctgcgaa aatcgccgca
     1981 cctacccagt ggttgtgcag gaaggccttg aagcaggcat cgcggtcgcg ggtgcgggcc
     2041 tgccacagct gccaagtcca caatgccact gccgccagca ggccggcgta gtaccagagg
     2101 ctgaactgta actggttgcc cagcagccag agcgcgaaca tggccaggcc gttgaggatg
     2161 cccaccatca tcaggtcggc gtcgccgaac aggatggcgg tggacttgat gccggcctgg
     2221 atatcatcgt cccggtccac catcgcatag agggtgtcgt atgccatggt ccaggacagg
     2281 gtggccatgt agatcagcca ggcgtgcagg ggcacgcttt cacccaccgc cgcgaaggcc
     2341 attggaatcg accaggcgaa cgctgccccc agtacgacct gcggcagata ggtatggcgt
     2401 ttcatgtagg gataggtgca ggccagcagc agtccgccca ccgacagcac gatggtgaac
     2461 aggttggtga acagcaccag cacaaaggcc agcaggccga tcagtgccgc gacagcgagc
     2521 gcttccttgc cgctgatgcg cccggtggcc aggggacggt tggcagtgcg ctgcacctgg
     2581 ccatcgaagt cgcggtcggc atagtcgttg atgacgcagc cgccggtgcg cgtgaggatc
     2641 acccccatca cgaaaatgaa gatcagcttg aaggagggtt tgccttccgc ggcgatccac
     2701 aacgcccaca gggcgggcca cagcagcagg tagatgccga tgggccggtc caggcgtccc
     2761 agctggatga aatccggcag ccggcgcagg ggcgagttgg acagttgcag ttccttcaat
     2821 gtggccacgg cgggctcctg ggttgggcgt gggacgggtc agggctgggt gaaacgggct
     2881 tggcggatta tcgtcattcc cgccccccgg gtacaaccgg agcgtacatc gggcaggcct
     2941 ccaggaagta ctcactcacc agcaggggca gacccg
//
LOCUS       DASUEG010000950         7828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1052373, whole genome shotgun sequence.
ACCESSION   DASUEG010000950 DASUEG010000000
VERSION     DASUEG010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052373"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VFX11_17895"
     CDS             complement(<1..173)
                     /locus_tag="VFX11_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4940550.1"
                     /translation="MSIKVAIRHHTRYSYDRAVQLFPHIFRLRPAVHSRTPIEGYSLN
                     IKPDNHFINWQQDP"
     gene            complement(445..762)
                     /locus_tag="VFX11_17900"
     CDS             complement(445..762)
                     /locus_tag="VFX11_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940551.1"
                     /translation="MQNGSTRTYANVDSASSEGLELAGRWQPIAALQLRTTLTPVAGE
                     VLELDSCWQLHGKLLFDLGDGVQWWLQAENLPDEEFAASAQGNGMEEGVPNRGREFGA
                     GVL"
     gene            complement(833..2803)
                     /locus_tag="VFX11_17905"
     CDS             complement(833..2803)
                     /locus_tag="VFX11_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940552.1"
                     /translation="MPMISSLDLTGAIAAALATLFLLTHWRRRLATEEKWIVLIVCAL
                     TVLINGISVWSWSGQHFAGQVAENWGDYLQILQPALWGMFFYVVVQSALRKELKASRQ
                     RMRDLVENMPVILNAYDRDGGILAWNRTAQEVTGLSRDDVVGNSQALQQLFPDSQVQE
                     QLRMECEHGGGHYKHRIHTLKGRKEERQIAWYNIAQEFPISGWANWSIGLDMTEQLAA
                     QRQLEHLATHDELTGLANRALLHDRLHHALAASQRNNITGALLMLDLDHYKMVNDSHG
                     HPVGDQLLREVGKRLGGCLKSTDTLARIGGDEFMVLLDQIHEPEEAAIVAQRLLSALD
                     EAPFQLFGNNIRTRASIGITIFPDDDIRLDELMKNVDLALYTAKQNGRNTFHFYSRNL
                     HTQFRWQHQVAEKLRDAISNDALLLHYQPQIALRDHRVAGVEALLRWPAFDNGSLSPA
                     VFVPIAENNGLMPDLGRWVVENAFRQAATWQAQHGPMTTAVNLSAVQLYQPNLADFLL
                     QLLERLQLNAENIDLEITESAVMRNVDDAIAAMKQLRDKGFHISLDDFGTGYSSLSYL
                     KRFPVSRIKIDQSFVQGMDTSAGDIAIVRNVIRLGHDLGLLVLAEGVETRQQLQRLGD
                     EGCDLVQGFFCSKPLAAGPLGEFLRQGGGLVH"
     gene            complement(2790..3074)
                     /locus_tag="VFX11_17910"
     CDS             complement(2790..3074)
                     /locus_tag="VFX11_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940553.1"
                     /translation="MSENEILVLLVGTLVLGFVLLYRRDIQRLPAYHYLFAAYLSAWI
                     AWVATVAEHVFLYSFFNVLEHVGYAANGVLLLIWCWKGLRNGAHERHAHD"
     gene            complement(3331..4983)
                     /locus_tag="VFX11_17915"
     CDS             complement(3331..4983)
                     /locus_tag="VFX11_17915"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4940554.1"
                     /translation="MRNFVHAGRALPVLLPLLLAAPAHGQLLTNLSIGNPKGLALANA
                     VTADPPGIDSIHFNPAGLAKIQGRQANLKLLIAQVDLESRFGEPIKPNEAEKQAYYNV
                     NEQCQTDYPAGDADAISQCWGVDPVAGASTESGDPVVMIPFIGVQEVPILAFPMGGMA
                     FEDAKNRWVLGSAVYVPEGIGLTREMEGPGAYQGQEVALTRLTYFSPTIGLSIAENLF
                     VGLGLNLSYQGLYVRTKFRAPTLTLGYLRDLNNIAGSPLPPIEFGPYDSAGLLTLELE
                     DMLSVGFNFGLLWEPKPWLALGFVYHSESKSDLSGDFKMENSELFLKTTTGLSQDPLV
                     SGLIVALGGAPLNAQAVEKGSVELEYITPQNLAFGASIRLLPDLKLNVDAKWANYSRW
                     NDLTFYFDQDVDFLSFGTAISTAAGYNLTSPEMMVITREYEDTWSWAVGLEYQLNANW
                     VARVGYEPRTSAIPDDRTDLIFPLGEMDLYGAGLGWQFDQTTRFDAAIAYLHSETSTP
                     ACGSQNANSCEEGNVVYNPYYATEFENEVNGILVALSVDRKF"
     gene            complement(5157..5927)
                     /locus_tag="VFX11_17920"
     CDS             complement(5157..5927)
                     /locus_tag="VFX11_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4940555.1"
                     /translation="MQPTETRHFYLIRGLIREARHWGVLPDLLRQAFPGAQVFTLDIP
                     GAGDYFRQASPLSVDAMVEHMRADFLQRSRAGENNTLIAISLGGMIAARWLQAHPQDF
                     QRAVLINTSFGGLSPLYHRLRPGAVLSMLRAAVTPPRQREGVILRLVSNNADVFDEGL
                     ALWDSIRRERPVSAANAARQLWAASWFQLPAVRPAVPVLLLASTHDRMVSVECSRAIA
                     RAWQAPLREHSSGGHDLSLDDPGWILEQVRRFPETVPL"
     gene            6078..7103
                     /locus_tag="VFX11_17925"
     CDS             6078..7103
                     /locus_tag="VFX11_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4940556.1"
                     /translation="MDHLPVGTVRIGYVRALLDYLQNHDIDVSPVFAADLLAELDAPS
                     LNERLSVQRWGELLERAIAHTGDAALPLHIAEELTPRHWGVFAYAAMSCKTLAEVVVI
                     LERYERLIDEVNDTRMVLLGDKAGLQWLPRVKAPVPALMQLSLSSWCVFARRYTGHAE
                     LVADVHFTFPAPADIAPYQRIFGGTIAFSQPVTQLVFPLHYLQLPITHHDADTHRILL
                     SQAQSQFEALNAENSVQQQLRDAITRRLTSSGGVTLEQLAEDLGLASRTLQYQLETSG
                     TSYRQLLDEVRTQLARFYRSDPDMALVDVAFLLGYSEQSPFNKAFKRWTGETPGEYRK
                     RTGKPTS"
     gene            7231..>7828
                     /locus_tag="VFX11_17930"
     CDS             7231..>7828
                     /locus_tag="VFX11_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4940557.1"
                     /translation="MQRLPGIAALILSAMLSGNVSAACNNPTTYAKTKYPIVLVPGGF
                     GFVNLGGVLPYFYQIPEDLRCNGATVLTPEFSALATSEQRGEQLRAYLETYFALNPQV
                     EKVNLQSHSQGAGTIRYVAATMPGRVASVTAVAGNVNGNYVAQLYTDSANKLGLAGDF
                     VDWAVQEIGGALGILIGYLSAGETLPQDTLGTIHEISMD"
BASE COUNT         1603 a         2417 c         2306 g         1502 t
ORIGIN      
        1 gggtcctgct gccagttgat gaaatggttg tcgggcttga tgttgagcga gtaaccctcg
       61 atgggcgtgc ggctgtgcac ggccggccgc agccggaaaa tgtgggggaa caactgcacc
      121 gcgcgatcgt aggaatagcg ggtgtggtgg cggatggcaa ctttgatgga cataggcaac
      181 cttgtgtgat cagggtttcg cacagcatga gcagcgcgac ctgtagccta acgtgtttga
      241 gtggcccggt cgcgtgcaat gtcgggactg gagtgcagcg caagttctgt gccacggcgt
      301 gaaggggcag gaagtgcgga agcagcggtg aggatgcgcc agaaacgggc ccttccggcg
      361 ccggcacgca ccttcatgga gcctggctga gttttccagc ggattgtcat ggctggatca
      421 cgccaatccc gccgcaggca gccgctacaa tacccctgct ccgaattcac ggccgcggtt
      481 gggcacgccc tcctccatgc cattgccctg ggccgacgcg gcgaattctt catccggcag
      541 attttctgcc tgcaaccacc actgcacgcc atcacccaga tcgaacagaa gcttgccgtg
      601 cagctgccag caggaatcca gttccagcac ctcccctgcc accggtgtca gcgtggtgcg
      661 caactgcagc gccgcgatcg gctgccagcg ccctgccagc tccagacctt cgctggatgc
      721 cgaatcgacg ttggcatagg tacgcgtgct gccgttctgc acgatcgcca ccggatcgat
      781 atgccatccg gtcgaccact gcccggcgct tgcactggaa tttttattgc attcaatgca
      841 ccagcccgcc accctgccgc aagaattcac ccagtggccc ggccgccagt ggcttcgaac
      901 agaagaatcc ctgcaccaga tcgcaccctt catcacccag acgctgcaac tgctgccgtg
      961 tttccacccc ttctgccaat accagcagac ccagatcgtg gcccaggcgg atgacattgc
     1021 gcacgatggc gatatcaccg gcgctggtgt ccatgccctg cacgaaggac tggtcgatct
     1081 tgatgcgcga caccggaaaa cgcttcagat aactcagcga cgaatagccg gtgccaaaat
     1141 catccagcga aatgtgaaag cccttgtcgc gcaattgctt catggcggcg atggcatcat
     1201 ccacattgcg catcacggca gattcggtaa tttccagatc gatattttcc gcattcagtt
     1261 gcagccgctc cagcagttgc agcagaaaat ccgccagatt gggttggtac aactgcaccg
     1321 ccgacagatt gaccgccgtg gtcatggggc cgtgctgggc ctgccaggtg gctgcctgcc
     1381 ggaacgcgtt ttccaccacc cagcgcccca gatccggcat caggccattg ttctcggcaa
     1441 tcggcacgaa gaccgccggc gacagtgagc cgttatcgaa agccggccag cgcaacagcg
     1501 cctccacacc cgccacgcga tggtcgcgca atgcaatctg cggctggtag tgcaacagca
     1561 aggcgtcatt gctgatggcg tcccgcagct tttccgccac ctggtgctgc cagcgaaatt
     1621 gcgtgtgcag gttgcgcgaa tagaaatgga aggtattgcg gccgttctgc ttggcggtgt
     1681 acagcgccag atcgacgttc ttcatcagct cgtccagccg gatgtcgtcg tcaggaaaaa
     1741 tggtgatgcc aatgctggcg cgggtgcgga tgttattgcc aaacagttgg aacggcgcct
     1801 cgtccagcgc actcaacaga cgctgcgcca caatcgccgc ttcttcgggt tcgtgaatct
     1861 ggtccagcag caccatgaat tcgtcgccgc cgatgcgcgc cagggtatcg gtggatttca
     1921 gacagccacc cagccgcttg cccacttccc gcaacaattg atcacccacc ggatggccat
     1981 ggctgtcgtt caccatcttg tagtgatcca gatccagcat cagcagcgcg ccggtgatgt
     2041 tgttgcgctg gctggcggcc agggcatgat gcagacggtc gtgcagcaat gcgcggttgg
     2101 ccagcccggt gagttcgtcg tgggtggcca gatgttccag ctggcgttgg gcggccagct
     2161 gctcggtcat gtccagaccg atgctccagt tggcccagcc gctgatggga aattcctgcg
     2221 cgatgttgta ccaggcaatc tggcgttctt ccttgcgccc tttcagcgta tgaatgcgat
     2281 gcttgtagtg gccgccgccg tgttcgcact ccatgcgcaa ctgttcctgc acctgggaat
     2341 cgggaaacag ctgctgcagc gcctgggaat tgcctaccac atcgtcgcgg ctgaggccgg
     2401 tgacttcctg cgcggtacgg ttccacgcca ggatgccacc atcgcgatca taggcattga
     2461 gaatcaccgg catgttttcc accagatcgc gcatgcgctg gcggctggcc ttcaattctt
     2521 tgcgcagggc cgactgcacc accacataaa aaaacatgcc ccacagcgcc ggttgcagga
     2581 tctgcagata gtcgccccag ttttccgcca cctggccggc gaagtgctgc ccgctccacg
     2641 accacacgct gatgccgttg atgagcaccg tgagcgcgca gacgatcagt acgatccact
     2701 tttcctcggt ggccagacgc cggcgccagt gggtgagcag aaacagcgtc gccagggctg
     2761 ccgcaattgc gccggtcagg tccagtgaac taatcatggg catggcgctc gtgagctccg
     2821 ttgcgcaaac ctttccaaca ccagatcagc aacagcacac cgttggcggc gtaaccgaca
     2881 tgttccagca cgttaaaaaa tgagtagagg aaaacgtgtt cggcgacagt cgcaacccag
     2941 gcgatccagg ctgacaggta ggcggcaaac agatagtgat aggcgggcag acgctggata
     3001 tccctgcggt agagcagcac gaatcccaac accagggtgc ccaccagcag aaccagaatt
     3061 tcattttcgc tcattgctgc ttatccctcg cgtggcgtcc tgttcccgca tcgtctgctg
     3121 gtcagcctgc caggcaatac acggggcact ccgttcaaaa ttaggcagga gcgcaccaaa
     3181 aagcaacaga ttgcagaaag gcccggggct ggtggggaaa aatcatctaa tctggtgcag
     3241 gctaatagcc aatgatgaca gtcaggcccc acgttgcatg gggccatttg gccgtacggg
     3301 aaaccggtgc ctaagacagc ttggccggct tcagaacttg cgatccaccg acagtgccac
     3361 caggatgcca ttcacttcat tttcaaattc agtcgcgtaa taggggttgt agaccacgtt
     3421 accttcttcg cagctgttgg cgttctgtga tccgcaggcc ggcgtactgg tttccgaatg
     3481 caggtaggcg atggcggcgt cgaagcgggt ggtctggtcg aactgccagc ccaggccggc
     3541 gccatacaga tccatttcac ccaacgggaa aatcaggtcg gtgcgatcat ccggaatcgc
     3601 ggacgtgcgg ggttcatacc ccacccgcgc cacccagttg gcattgagct gatattccag
     3661 gcccaccgcc caggaccagg tgtcctcgta ttcgcgcgtg atcaccatca tctccggcga
     3721 cgtcaggtta taacccgccg cagtgctgat ggcggtaccg aagctgagaa aatccacgtc
     3781 ctgatcgaaa tagaatgtca ggtcattcca gcggctgtaa ttggcccact tcgcatcgac
     3841 attcagtttc agatccggca gcaaccggat cgatgcgcca aacgccaggt tttggggtgt
     3901 gatgtattcc agctccacac tgcccttctc cacggcctgc gcattcagcg gcgcaccccc
     3961 cagcgcaacg atcaggcccg acaccagcgg gtcctgactc aggccggtgg tggttttcag
     4021 aaacagctcg gaattttcca tcttgaaatc gccgctcagg tcggatttcg attcactgtg
     4081 atagacaaaa ccgagcgcca gccagggctt tggttcccac agcagaccga aattgaagcc
     4141 gactgacagc atgtcctcca gctccagcgt cagcagaccg gcactgtcat aggggccgaa
     4201 ctcgatgggc ggcagcggac tgccggcgat gttgttgaga tcgcgcagat accccagtgt
     4261 cagggtgggc gcgcggaatt tggtgcgcac atacaggccc tggtaggaca ggttgaggcc
     4321 cagcccgaca aacaggtttt ccgcaatcga caggccgata gtgggcgaaa aataggtgag
     4381 gcgggtgagc gcaacttcct ggccctggta cgcgcccggg ccttccattt cacgggtcag
     4441 accgatgcct tccggcacat acacggcact gcccagcacc cagcggtttt tcgcgtcctc
     4501 gaacgccatg ccacccatgg ggaacgccag gatcggcact tcctgcacgc cgataaacgg
     4561 aatcatcacc accggatcgc cggactcggt ggaggcgccc gccacgggat caacgcccca
     4621 gcactggctg atggcatccg catcaccggc gggataatcg gtctggcact gttcgttgac
     4681 gttgtagtac gcctgttttt cggcttcatt gggcttgatc ggttcgccga aacgcgattc
     4741 caggtccacc tgcgcgatca gcaatttcag gttcgcctgc cggccctgga tcttggccag
     4801 gccagcggga ttgaaatgga tggaatcgat gcccggcgga tcggcggtga ccgcattggc
     4861 cagggccaga cccttgggat taccgatgga gaggttggtg agcagctgac cgtgggcggg
     4921 agcagccaac agcaacggca acagcacagg caaagcccgg ccggcgtgaa caaaattcct
     4981 catgggatta cacctgtttt tttgttgttc tgatttgttg ttctatttgt ttttttggat
     5041 gaaacggcta ataagacaat tagtacgatg gtcccgattg accagattac actggctttc
     5101 agtgcgttgt cagtaggtgg aggagcggac acccgcgtcg cgccggcaaa tcgcggtcac
     5161 aggggaaccg tttccggaaa acgccgcacc tgttccagaa tccagcccgg atcgtccagc
     5221 gacagatcgt ggccgccgct gctgtgctcc cgcaaggggg cctgccaggc gcgggcgatg
     5281 gcgcgggaac attcgacact caccatgcga tcgtgggtgg acgccagcag cagcaccggc
     5341 accgccggcc gcaccgccgg caactgaaac caggatgctg cccacaactg tcgcgccgca
     5401 ttggcggctg aaaccggtcg ctcacggcgg atgctgtccc acagagccaa accctcatca
     5461 aacacatcgg cgttgttgct caccagtcgc aggatcacgc cttcccgttg ccggggcggg
     5521 gtgacagcgg cccgcagcat gctcagcact gcgcccggcc gcagccggtg gtacaggggc
     5581 gacaatccac caaaactggt gttgatcagc accgcacgct ggaaatcctg cggatgcgcc
     5641 tgcagccagc gcgccgcgat catgccgccc agcgaaatgg cgatcagcgt gttgttttcg
     5701 ccggcacggg agcgctgcaa aaaatccgct cgcatgtgct cgaccatggc gtccacgctc
     5761 aacggcgatg cctggcggaa atagtcgccg gcaccgggaa tatccagcgt aaaaacctgc
     5821 gcgcccggaa acgcctggcg cagcagatcc ggcagcacac cccagtgccg cgcctcgcgg
     5881 atcaggcccc ggatcagata aaaatgacgc gtttcagttg gctgcatgtc gatgccttgc
     5941 gttgcgggtt cacatagttg gtattatcac ggcgttcatt atgcagaatg tcggcgtggc
     6001 gaaaccatag cagccggtcc acctgccgta ttgattcaaa ccggcgtcaa ttgaccaaac
     6061 acgcaaaagg agaaggcatg gatcacctgc ccgtcggcac cgtgcgtatc ggttatgtgc
     6121 gtgcattgct ggattatctg cagaatcatg acatcgacgt gtcaccggtc tttgctgcgg
     6181 atctgctggc cgaactggac gcgccgtccc tgaatgaacg cctttccgtg cagcgctggg
     6241 gcgaactgct ggaacgcgcc atcgcgcaca cgggcgatgc cgcactgcca ctgcacattg
     6301 ccgaagaact cactccccgg cattggggcg tgttcgccta cgccgccatg agctgcaaaa
     6361 ccctggcgga agtggtcgtt attctggagc gctatgagcg cctgattgat gaagtgaacg
     6421 acacccgcat ggtgctgctg ggtgacaagg cagggttgca gtggctgccc cgggtgaagg
     6481 cgccggtgcc ggcactcatg cagttgtcgc tttccagctg gtgcgtattc gcgcgccgct
     6541 acaccggaca cgccgaactg gtggcggatg tgcacttcac gtttcccgca cctgccgaca
     6601 tcgcgcccta ccagcgcatt tttggcggca ccattgcctt cagccagccg gtcacccagc
     6661 tggtttttcc attgcactat ctgcaactgc cgatcaccca tcacgacgcc gacacccatc
     6721 gcatcctgct gtcacaggcg caaagccagt tcgaagcgct gaatgctgaa aattcggtcc
     6781 agcaacaatt gcgggatgcc atcacccggc gcctgaccag cagcggcggc gtcacgctgg
     6841 aacaactggc cgaggatctg gggctggcgt cccgcaccct gcaataccag ctggaaacca
     6901 gcggcaccag ctaccgccag ctgctggacg aagtgcgcac gcaactggcg cgcttctacc
     6961 ggagcgaccc ggacatggcg ctggtggacg tggcgtttct gctgggctat tccgagcaga
     7021 gtccgttcaa caaggcgttc aagcgctgga ccggggaaac gcccggggaa tatcgcaagc
     7081 gcacaggcaa acccacgtcc tgattgcgcg aaaggacaag cgtctgcgtg gttttggaca
     7141 atacggctgc ctggcggcgg aataacgttg caatcctggg ttacgcattc cctgcacacg
     7201 tattccaata acaaaaacga ggacgccacc atgcaacgcc tgccgggcat cgctgccctg
     7261 atcctgtctg caatgctgag cggtaacgtc agtgccgcct gcaacaatcc caccacgtac
     7321 gcaaaaacca aataccccat cgtgctggtg cccggcggct tcggcttcgt caacctgggt
     7381 ggcgtcctgc cgtatttcta ccagatcccc gaggatctgc gctgcaacgg cgccaccgtg
     7441 ctgacgccgg aattttccgc gctggccacg tcggaacagc gcggcgaaca attgcgggcc
     7501 tacctggaaa cctatttcgc gctgaatccg caggtggaga aagtcaatct gcaaagccac
     7561 agccagggcg ccggtaccat ccgctacgtg gccgccacga tgccggggcg cgttgcatcg
     7621 gtgaccgccg tggcaggcaa tgtcaacggc aactacgtcg cgcaactcta taccgacagt
     7681 gccaacaaac tggggctggc gggggatttc gtggattggg cggtgcagga aatcggtggc
     7741 gccctcggca ttctgatcgg ctatctgtcg gcaggcgaaa cgttgccgca ggacacactg
     7801 ggcaccatcc atgaaatcag catggacg
//
LOCUS       DASUEG010000951         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_345469, whole genome shotgun sequence.
ACCESSION   DASUEG010000951 DASUEG010000000
VERSION     DASUEG010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345469"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFX11_17935"
     CDS             complement(<1..515)
                     /locus_tag="VFX11_17935"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX4940558.1"
                     /translation="MYVPEHFAVKDTDVLYDWIERWSFGTLITSHRGRMEANQIPFLV
                     DRERGDLLGHMARSNKQWQTLLNAEHLLVLFNGPHGYVSPAWYSQPDGVPTWNYLTVQ
                     VQGKAELVKTESEAIDILRRLTNTNEALYGPGWRLEDLDETRLKAMTQAIVCFRVHIS
                     ALEGKAKLSQNR"
     gene            680..1693
                     /locus_tag="VFX11_17940"
     CDS             680..1693
                     /locus_tag="VFX11_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX4940559.1"
                     /translation="MKRIVITRHGGPEVLQVQESPDPKPRAGEVLIRVKAAGLNFADV
                     LARKGLYPDAPATPCTVGYEVAGVVEAVGSAAEESWLQQDVIALTRFNGQSDAVVVPV
                     SQVFAKPAGLSFELAASIPVNYLTAWQLLVAMGGLQAHESVLIHNAGGGVGLAALDIA
                     VHVGATTLGTASPGKHAFLRERGLQHPIDYRTGDWGPVVQRITQGKGVDLITDPIGGS
                     HFRKSYRHLRSTGRLGMFGISTVADGLGGKLALVKMALQTPLFHPFGLMNSNKGVFGV
                     NMGHLWHEQEKVRLWTDAILKGYAEGWVRPHVDRVFRFEQAADAHRHMENRGNTGKVV
                     LVP"
     gene            complement(1804..>2422)
                     /locus_tag="VFX11_17945"
     CDS             complement(1804..>2422)
                     /locus_tag="VFX11_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4940560.1"
                     /translation="KTRSDRKLETRRRILDAALQLVEEGRPPEALGLREVARVAGVAA
                     PSIYNHFADMDELGLALVDDSCIRLRSLARTARQPMAEHDMPQALKRLLEQFLQYVNR
                     HESILRLLIQQWFNPNPAFRRTIRRELASMQVDLADSMRQVAAQKGMRSGDYSLESQA
                     ILSLLTVFVLDAMDLDREQRERRMERLERQILMLVLGSRALATGG"
BASE COUNT          445 a          780 c          768 g          429 t
ORIGIN      
        1 cggttctggc tgagcttggc cttgccttcc agagcgctga tatgaacgcg gaagcagacg
       61 atggcctggg tcatggcctt gagccgcgtc tcgtccagat cctccagacg ccagccgggg
      121 ccgtagaggg cctcgttggt attggtgagg cggcgcagga tgtcgatggc ctcgctctcc
      181 gtcttcacca gttccgcctt cccctgcacc tgcacggtca ggtagttcca cgtgggcacg
      241 ccgtccggct gactgtacca ggccggcgac acgtagccgt gggggccgtt gaacagcacc
      301 agcaggtgct cggcgttgag cagggtctgc cactgcttgt tgctgcgggc catgtggccc
      361 agcagatcgc cgcgttcgcg atccaccaga aagggaatct ggttcgcctc catgcggcca
      421 cggtggctgg tgatcagagt gccgaaactc caacgctcta tccagtcata gaggacgtcc
      481 gtgtccttga ccgcgaaatg ttcgggtaca tacatgggac aacttccaga gaaacagggg
      541 caatcaggca acgatagtga gttgcatgaa tggtaggtgg aagctgcccg caaaacaaac
      601 gctccgcctc agtttgacag ttatcgaact atcatatagt ttcgatccac ccccgcgaac
      661 caccactgga gattccccca tgaaacggat tgtcatcacc cgccacggcg gccctgaggt
      721 actgcaggtg caggaaagtc ccgatcccaa accccgcgca ggggaagtgc tgatccgggt
      781 aaaggcggcg ggcctgaatt tcgccgacgt gctggcccgc aaggggctgt atccggatgc
      841 gcccgcgacg ccctgcaccg tcggctatga agtggcaggc gtggtggaag cggtgggcag
      901 tgcggcggaa gaatcctggc tgcaacagga cgtcatcgcc ctgacccgct tcaacggcca
      961 gtccgacgcg gtggtggtgc ccgtgagcca ggtgttcgcc aagcccgccg gcctgtcctt
     1021 cgaactggcg gcgtcgattc cggtgaatta cctcacggcc tggcagctgc tggtggccat
     1081 gggcgggctg caggcccacg aatcggtgct gatccacaat gccggcggcg gcgtggggct
     1141 ggcggcgctg gatattgccg tccatgtcgg cgccaccacc ctgggcaccg ccagccccgg
     1201 caaacatgcc tttctgcggg agcgcggcct gcaacatccc atcgactacc gtaccggcga
     1261 ctggggtccg gtggtgcaac gcatcaccca gggcaagggc gtcgacctga tcaccgatcc
     1321 catcggcggc agccacttcc gcaaaagcta caggcacctg cgcagcaccg gccggctggg
     1381 catgttcggc atctccaccg tggcggacgg tctgggtggc aagctggcgc tggtgaaaat
     1441 ggcactgcaa accccgctct tccacccctt tggcctgatg aacagcaaca agggtgtgtt
     1501 cggcgtgaac atgggccatc tgtggcacga gcaggagaaa gtgcggctgt ggacggatgc
     1561 gattctgaag ggttatgcgg aaggctgggt gcgcccccat gtggaccggg tgttccgctt
     1621 tgaacaggcg gcggacgcac accggcatat ggaaaaccgg ggcaatactg gcaaggtggt
     1681 gctggtgccc tgaacaggga ataagagcag cccgccgtcg gcatacactg agtgatggca
     1741 cccggctgag ccgtggcgcc atcaggccct atccatcagg ccctatccat cgtcccctat
     1801 ctatcagcca cctgttgcca gcgcccggct gcccagtacc agcatcagta tttgccgctc
     1861 caggcgctcc atccggcgct cgcgctgttc cctgtccaga tccatggcat ccagcacaaa
     1921 caccgtcagc agcgacagaa tggcctggga ttccagcgag taatcgccgg accgcatccc
     1981 cttctgcgcg gctacctgcc gcatgctgtc cgccagatcc acctgcatgg acgccagttc
     2041 gcgccggatg gtgcggcgaa acgccgggtt cggattgaac cactgctgga tcagcaggcg
     2101 caggatggac tcgtgccggt tcacatactg caggaactgc tccagcagac gcttcagtgc
     2161 ctggggcatg tcgtgctccg ccattggctg ccgcgccgtg cgggcaagcg aacgcaggcg
     2221 gatgcaactg tcatccacca gcgccagacc cagttcgtcc atgtcggcaa agtggttgta
     2281 gatggaaggc gccgcaaccc ccgccacccg cgccacttcg cgcagcccca gcgcctcggg
     2341 cgggcgcccc tcctccacca gctgcagcgc ggcatccaga atacgccggc gggtttccag
     2401 cttgcggtcg gagcgggtct tg
//
LOCUS       DASUEG010000952         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1064389, whole genome shotgun sequence.
ACCESSION   DASUEG010000952 DASUEG010000000
VERSION     DASUEG010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064389"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFX11_17950"
     CDS             <1..297
                     /locus_tag="VFX11_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940561.1"
                     /translation="RFPIDTLKIDRSFIRDLEQDPESILVLESIISLAQNLKLEIVAE
                     GIETEHQLTILEQRGCDFIQGFYFAKPTPAPESMQFFERYFSRQQDVALEKKAG"
     gene            complement(361..678)
                     /gene="grxD"
                     /locus_tag="VFX11_17955"
     CDS             complement(361..678)
                     /gene="grxD"
                     /locus_tag="VFX11_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX4940562.1"
                     /translation="MDTLDVIKDQISSNNVILYMKGTPQFPQCGFSARAVEALMSVGK
                     PFAYVNILEHPDIRSELPKFANWPTFPQLWVKGELVGGSDIVLEMAQKGELKPLIEEA
                     TGE"
     gene            complement(830..2932)
                     /locus_tag="VFX11_17960"
     CDS             complement(830..2932)
                     /locus_tag="VFX11_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX4940563.1"
                     /translation="MTDSIQTHYRACHLCEAICGLEIKTQGDRVLSIKGDDNDPLSRG
                     YICPKATALQDIHEDPDRLRQPLRRVGDQWQPISWDAAFDLAAEKLVAVQQQHGGNAI
                     GIYMGNPSVHNWGMTTHAANFFKPLKTRNRFSATSVDQLPHQLLCYWMYGHQVLVPVP
                     DIDRTQLLIIVGGNPMASNGSLWTVPGFRHRAKALQKRGGQLVVIDPRRSETADIADQ
                     HHFVRPGSDAFLLLAMVREIFANNWVKAGHLGESLLGLEEVKHAVEPFSLELAAQQTG
                     MSAAAIRELTRQFAHTENAVIYGRMGISVQRFGSICQWAIQLLNILTGHLDVPGGSMF
                     SLPAVDLVWGPNSKPGHFNAWKSRVRGLPEFGGELPCSALAEEIATPGDGQIRALITG
                     AGNPVLSNPNGQQLEKALGSLEFMLSLDIYLNETTRFADLILPSTSPLEHDHYDLALQ
                     VFAVRNTAKYSPAIFPKPEGAMHDWEIMSAIGERISSLLGQKPLPVFPPDMILDAGLR
                     AGPYGKDLSLQVLKDNPHGVDLGEHTTCLPQRLYHEDKKIRCAPDAVLADLPRLLAES
                     SRADAAGFRLIGRRHIRSNNSWLHNSQRLVKGPLRSQLLMHPDDLARLGVSSGHNVKV
                     ASRVGSVVVPVEASDEIMPGVVSLPHGWGHNRAGIRIGVAQEHAGVSVNDLTDEQYLD
                     DLSGNAALNGVPVSVTAA"
     gene            complement(3066..>4394)
                     /locus_tag="VFX11_17965"
     CDS             complement(3066..>4394)
                     /locus_tag="VFX11_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4940564.1"
                     /translation="PFRGGTHLPDVTVIAPVFVHGQLIAFVANRAHHANIGASAPGSM
                     PVSRTLEEEGVVIPPTHLLRNGVWEWPVLERLAGLAPGALQQQQDDGGSAGGAVELSA
                     AVGDFFAQVSANRVGVLRLENLIAQYGVAVFSTLVDALNDYAERLALASLRTLPAGRY
                     SFTDYLDDDGAGTQRIAITCAVIVTEQGVVVDFTGTAPQVAGNVNCPLSVAAAAVFYV
                     LRCLMPEQTPACAGTFRPIQFLAPEGSLLNARYPAAVAAGNVETSTRVVDVLLGALAQ
                     AVPQRIPAASHGSMNNLAMGALLADGRRWDYYETIGGGMGASALGDGISAVQTHMTNT
                     LNTPVESLEAHYPLRVKRYGVRRGSGGAGAHRGGDGIVREIEFLQPATVTLLTERRVS
                     CPWGLSGGASGAAGINLLNGHSLPAKITFSVQPQDVLTVATPGGGGWGAA"
BASE COUNT          894 a         1434 c         1237 g          829 t
ORIGIN      
        1 cgcttcccca tcgacacgct gaagatcgac cgcagtttca tccgcgatct ggagcaggac
       61 cccgaatcca tactggtgct ggaatccatc atcagcctgg cgcagaacct gaagctggag
      121 atcgtggcgg aaggcatcga aaccgaacac cagctgacga tcctggaaca gcgcggttgc
      181 gatttcattc agggctttta tttcgcgaag ccaacaccgg cgccggaatc aatgcaattc
      241 ttcgaacgct atttttcgcg ccagcaggat gtcgcgctgg agaaaaaagc tggctaatgc
      301 ccaataaaaa acccgtcaca ttggacgggt tttttatttt ctgccgcggg tcgcaccggg
      361 ttattcgccc gtggcttcct cgatcagcgg tttcagttca cctttctgcg ccatctccag
      421 cacgatatcg ctgccgccga ccagctcacc cttcacccac agctgcggga aggtcggcca
      481 gttggcgaat ttcggcagct cggaacggat atccgggtgc tccaggatat tcacatacgc
      541 aaacggcttg ccgacggaca tcagggcttc caccgcgcgg gcggaaaaac cgcactgggg
      601 aaactgcggt gtgcctttca tatagaggat gacgttgttg ctgctgatct ggtctttgat
      661 gacgtccagg gtatccatgt tctcacctgc tgttggaaaa ccgggccgca cagacaccgg
      721 caacccgaaa ctggcggcca ttgtaacatg attttgtaac cggattgttc atggctgacc
      781 gcctgaaaaa caccgcactg cccgcaggga ttttacccat cctcccggat caggccgccg
      841 tcaccgacac cggcacgccg ttgagcgcgg cattgcccga caaatcatcc agatactgtt
      901 cgtcggtcag atcattcacg cttacgccgg catgctcctg cgccacgccg atgcggatgc
      961 cggcacgatt gtggccccag ccatggggca gactcaccac cccgggcatg atttcgtcac
     1021 tggcttccac cggcaccacc accgacccca cccgcgatgc caccttcacg ttgtgcccgc
     1081 tgctcacccc cagtcgtgcc agatcgtccg ggtgcatcag cagctggctg cgcagcggcc
     1141 ctttcaccag acgctgggaa ttatgcagcc aggaattgtt gctgcggata tggcgacggc
     1201 cgatcaggcg gaaaccggct gcatcggcgc ggctgctttc cgccagcaga cggggcagat
     1261 ccgcgagcac ggcgtccggc gcgcaacgga ttttcttgtc ctcgtgatac agacgttgcg
     1321 gcaggcaggt ggtgtgttcg cccagatcaa cgccgtgggg attgtccttc agcacctgca
     1381 gcgacagatc cttgccataa ggcccggcgc gcaggcccgc atccagaatc atgtccggcg
     1441 ggaacaccgg cagcggcttc tgccccagca gactggaaat gcgctcgcca atcgccgaca
     1501 tgatttccca gtcgtgcatg gcaccttccg gtttcggaaa aatcgccggg ctgtatttgg
     1561 cggtattgcg caccgcaaac acctgcagcg ccagatcgta atgatcgtgc tccagcggac
     1621 tggtggatgg caggatcaga tcggcaaagc gcgtggtttc attcaggtag atatcgagtg
     1681 acagcatgaa ttccagcgac cccagcgctt tttccagctg ctgcccgttg ggattggaca
     1741 acaccggatt gccggcaccg gtgatcagcg cgcgaatctg gccatcaccg ggcgtggcaa
     1801 tttcctccgc cagcgccgag cacggcagtt cgccaccaaa ttccggcaaa ccgcgcacgc
     1861 ggctcttcca cgcattgaaa tggccgggct tgctgttggg gccccacacc agatccaccg
     1921 ccggcaggct gaacatggaa ccgccgggca catccagatg gcccgtgaga atattcagca
     1981 gctggatcgc ccactggcag atggaaccga aacgctgcac ggaaatgccc atgcggccgt
     2041 agatcacggc gttttccgtg tgggcgaact ggcgggtcag ttccctgatc gcagcggcgg
     2101 acatgccggt ctgctgcgcc gcaagttcca gcgaaaatgg ttccactgca tgtttgactt
     2161 cttccagacc cagcagactt tcgcccagat gccctgcctt cacccagttg ttcgcgaaaa
     2221 tttcgcgcac catcgccagc agcaaaaagg catcgctgcc gggacgtacg aaatgatgct
     2281 ggtcagcgat atcggcagtt tcgctgcggc gcggatcgat caccaccagc tggccaccgc
     2341 gtttctgcaa tgccttggca cgatggcgaa aaccgggcac ggtccacagg ctgccgttgg
     2401 acgccatggg attgccgccg acgatgatca gcaactgcgt gcgatcaata tctggcaccg
     2461 gcaccaacac ctgatggccg tacatccagt agcacagcaa ctggtgcggc agctggtcca
     2521 ccgacgtggc ggaaaagcgg ttgcgggttt tcagcggttt gaaaaaattc gccgcgtgcg
     2581 tggtcatgcc ccagttgtgc acgctgggat tgcccatgta gatgccgatg gcattgccac
     2641 cgtgttgctg ctgcaccgcc accagttttt ccgctgccag atcgaaggcg gcgtcccagc
     2701 tgatgggttg ccactggtca cccactcgcc gcagaggctg acgcagccga tcgggatctt
     2761 catgaatgtc ctgcagcgcg gtggccttgg ggcagatata accgcggctg aggggatcgt
     2821 tgtcatcgcc cttgatggaa agaacgcggt cgccctgggt cttgatctcc agcccgcaga
     2881 tggcttcgca cagatggcag gcgcggtagt gggtttggat gctgtcagtc atggtgcgtc
     2941 tctctctact tttatcccgg tagtaactgc tgttgttgta atgggaacag gaaaaaaaat
     3001 ccggccaact tggataggta caactgctgc ctgggtaaaa ccactggaat tggctcaatc
     3061 gccagtcagg ccgcacccca tccaccacca cccggcgttg ccaccgtcag cacatcctgc
     3121 ggctgcacgc tgaaggtaat ttttgccggc agcgaatggc cattcaacaa attgattccc
     3181 gctgcacctg acgcgccacc cgacaggccc caggggcatg acacgcgccg ctccgtcagc
     3241 aatgttacgg ttgccggctg caagaattcg atctcgcgca cgataccatc gccgccacga
     3301 tgcgcacccg caccgccgga gccacgccgc acgccgtaac gtttcacccg cagcggataa
     3361 tgcgcttcca gactttccac cggcgtattc aacgtattgg tcatgtgcgt ctgcaccgca
     3421 ctgatgccat cgcccaacgc cgacgcgccc atgccaccgc caatggtttc gtaataatcc
     3481 cagcgccggc catccgccag cagtgcgccc atcgccagat tgttcatgct gccgtgactg
     3541 gcggcgggaa tccgctgcgg aactgcctgt gccagcgcgc ccagcagcac atccaccacg
     3601 cgggtgctgg tttccacgtt acctgccgcc accgcagcgg gataacgggc attgagcagg
     3661 ctgccttccg gcgccagaaa ttgaattggc cggaaagtac cggcgcaggc gggggtctgt
     3721 tccggcatca ggcagcgcaa tacatagaac accgcagcag ccgccaccga cagcggacaa
     3781 ttcacattgc ctgccacttg cggcgccgtg ccggtgaagt ccaccacaac gccctgctcc
     3841 gtcacaatta ccgcgcaggt aatcgcgatg cgctgcgtgc ccgcgccatc gtcatccaga
     3901 taatcggtga aggaataacg tcccgcaggc agcgtgcgca aactggccag cgccagccgt
     3961 tccgcgtaat cgttcagtgc atccaccaaa gtggaaaaca ccgccacgcc gtattgcgcg
     4021 atcaaatttt ccagccgcag cacgccgaca cgattggcgc tcacctgggc gaagaaatcc
     4081 cccaccgcag ccgacagttc aaccgctccg ccagcgctgc caccatcatc ctgctgttgc
     4141 tgcaacgcgc ccggtgccaa cccggccagc cgctccagca ccggccattc ccacacacca
     4201 ttgcgcagca gatgcgtcgg cgggatcacc acaccctcct cctccagcgt gcgcgacacc
     4261 ggcatggacc ccggcgcact ggcaccgata ttggcgtgat gggcgcgatt ggccacaaac
     4321 gcaatcagct ggccgtgcac gaacactggc gcaatcaccg tcacgtccgg cagatgggtg
     4381 ccaccccgga acgg
//
LOCUS       DASUEG010000953         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2046599, whole genome shotgun sequence.
ACCESSION   DASUEG010000953 DASUEG010000000
VERSION     DASUEG010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046599"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..713)
                     /locus_tag="VFX11_17970"
     CDS             complement(57..713)
                     /locus_tag="VFX11_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX4940565.1"
                     /translation="MLDTVFPDQIFYRVAEPWMWETPVNAAEEALVEKAVDKRKREFR
                     AGRHAAHALFRQVGADCPALLRGMQREPGWPAGWTGSISHTSGICVAAIASTSRYASL
                     GVDVEQATPLKPDILDMICSKDEQIHLRDIGRLAGTGAALDKLVFSAKESVHKTYFPL
                     NGHTLDFLDARIELDWGNKTFNAFILNPEANPRRPIRQLQGRFLFHGEWVVTCIALPA
                     "
     gene            complement(717..1709)
                     /gene="galE"
                     /locus_tag="VFX11_17975"
     CDS             complement(717..1709)
                     /gene="galE"
                     /locus_tag="VFX11_17975"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX4940566.1"
                     /translation="MAHGTILVTGGAGYIGSHVVRQLGERGEKIVVLDNLSTGFRDAV
                     LYGTLVEGDTGDMALVSRVLQEHQIETVMHFAAHTIVPESVTNPLKYYRNNTANTRNL
                     LECCQNHGVKHFVFSSTAAVYGEPEIPDITEESPTQPINPYGMSKLMSEIMLRDLAHA
                     CPMRHVILRYFNVAGCDPGGRIGQNTEKATLLTKVAVEAAVGKRQQVYIFGTDYPTPD
                     GTGVRDYIHVDDLASAHLKALDYLRAGGESITLNAGYGHGYSVREVLDMVGKVAGKPL
                     TIIETERRAGDPAALIATAGKIRKILGWEPRLDNLEEIVRSALAWERKLQAKRG"
     gene            1843..>2454
                     /locus_tag="VFX11_17980"
     CDS             1843..>2454
                     /locus_tag="VFX11_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940567.1"
                     /translation="MSRFSRSRAFLRNAVVGLATFCLVPDGPGSAQAHEVDTCLARLS
                     QTAQLLKACQIAQSSADQCDKLKARLERNRQTCRAEAFPEESIEHAISYGRGEVEGAV
                     ERSPYRQNVEQRRREQSQMQPNLVRFGKLFPQYAHFRDAVSERFDSRLCPNAYEGSRD
                     RWLYTGSMTLLQYSLSDVKSEPPVNREMHFFAQGKTGVCYGVKA"
BASE COUNT          486 a          725 c          737 g          506 t
ORIGIN      
        1 ggcgtggcgg aaaattcaaa cgaagcagtc gtcatgacgt taccttatgg ttcaattcag
       61 gcagggagcg cgatgcaggt aaccacccat tccccgtgaa acagaaagcg accctgcagt
      121 tgccggatgg gacgacgcgg attggcttcg ggattgagga tgaacgcgtt gaaggtcttg
      181 ttcccccagt ccagctcgat ccgggcatcc agaaaatcca gagtgtgacc attgagcggg
      241 aaataggttt tgtgaaccga ttccttggcg ctgaacacca gcttgtccag cgctgccccg
      301 gtgcccgcca gacggccaat gtcgcgcagg tggatctgtt cgtccttgct gcagatcatg
      361 tccagaatat cgggtttgag cggcgtggcc tgctcaacat ccacccccag gctggcgtac
      421 cggctggtag aggcaatggc cgccacacag attccactgg tgtggctgat actgccggtc
      481 cagccggcgg gccaacccgg ttcccgctgc atgccacgta gcagggcggg gcagtcggca
      541 cccacctggc ggaacagcgc atgggccgca tggcgccccg cgcggaattc gcgcttgcgc
      601 ttgtccacgg ctttttccac cagcgcctct tcggccgcat tgacgggggt ttcccacatc
      661 cagggctcgg ccacgcgata gaaaatctgg tcaggaaaaa cggtatccag catggatcag
      721 ccgcgctttg cctgcagttt gcgctcccag gccagggctg agcgcacgat ttcctcaagg
      781 ttgtcgaggc ggggttccca acccaggatc ttgcggattt tcccggcggt ggcgatcagg
      841 gcggcagggt caccggcccg gcgctcggtt tcaatgatgg tgagcggctt gccggccact
      901 ttgccgacca tatccagcac ttcgcgcacg ctgtaaccgt ggccgtagcc ggcgttcagc
      961 gtgatggatt ccccgcctgc ccgcagataa tccagggctt tcaggtgggc gctggccagg
     1021 tcatccacat ggatgtagtc gcgcacgcca gtgccatctg gggtcgggta atcagtaccg
     1081 aagatataca cctgctgacg cttgcccacc gcagcttcca ccgccacctt ggtgagcagg
     1141 gtggctttct cggtgttctg gccgatgcgg ccgccgggat cgcatcctgc cacgttgaaa
     1201 tagcgcagga tcacgtgccg catcgggcag gcatgggcca gatcccgcag catgatttca
     1261 ctcatcagct tggacatgcc gtaaggattg atgggctggg tgggggattc ctcggtgatg
     1321 tcgggaattt ccggctcgcc atagacagcg gcggtggacg agaacacaaa atgcttcacg
     1381 ccatggttct ggcagcattc cagcagattg cgggtgttgg cggtgttgtt gcggtaatac
     1441 ttgagcgggt tggtaaccga ttcgggcacg atggtgtgcg cggcaaaatg catgacggtt
     1501 tcaatctggt gctcctgcag aacgcggctt accagggcca tgtcgccggt gtcgccttca
     1561 accagggtgc cgtacagaac ggcatcgcga aaaccggtgg acagattgtc gagcacgacg
     1621 attttttcgc cccgctcacc caactgccgc actacatgac tgccgatgta accggcgccg
     1681 ccggtcacca gaatggttcc atgtgccatt ttttcactcc agctagtaaa aaaacccgcc
     1741 aaatcaatat ctaatcagta tatcacggaa ggcgtgaccg cttcagttct gtcgcgcgcc
     1801 gccgactata gttaattcag aatacaattt cacgagttac ccatgtcccg attctctcgc
     1861 agccgtgctt ttttgcgtaa cgccgtcgtc ggtctggcga cgttctgcct cgtccccgat
     1921 ggacccggct cagcccaggc acatgaggtt gacacctgtc tggcccggtt gtcccagact
     1981 gctcagctcc tgaaagcatg ccagatcgcc cagtccagcg ccgatcagtg tgacaaactc
     2041 aaggcccggc ttgaacgcaa ccgccagacc tgccgtgccg aagcgtttcc ggaagaaagc
     2101 attgagcatg ccatctcgta cggcaggggc gaggtggaag gcgccgtgga gcgcagtcct
     2161 taccgtcaga acgtcgagca acgtcgccgg gagcagagcc agatgcaacc caatctggtc
     2221 cgcttcggca agctgtttcc gcaatatgcc catttcaggg acgcagtgtc ggagcgcttt
     2281 gacagccggt tgtgcccgaa tgcctatgag ggaagtcgcg accgctggct ctataccggc
     2341 tcgatgacat tgctgcagta cagcctgagt gatgtgaagt ctgagccccc cgttaaccgg
     2401 gaaatgcact tttttgccca aggcaagaca ggggtatgct acggagtgaa ggca
//
LOCUS       DASUEG010000954         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_738837, whole genome shotgun sequence.
ACCESSION   DASUEG010000954 DASUEG010000000
VERSION     DASUEG010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738837"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFX11_17985"
     CDS             <1..1479
                     /locus_tag="VFX11_17985"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4940568.1"
                     /translation="RRGGAFFMRRSFKGNRLYSAVFNEYLNMVFDRGFSVEYFIEGGR
                     SRTGRLLEPRAGMLNMTVRSFLRTRRRSFVFIPVHFSYEKVLEGGTYLGELHGKAKKK
                     ESVFDIFRTLRKIKGTFGQVHVNVGEPIHLNQLLGDIAPGWRSEAYDDNEPPSWLNDS
                     VNELGNRIITHINDAAVVNPVNLLCLTLLSTTKLAMDEKALAKQLDFYLTFLRRIPYS
                     GIAVVPSLSGSDIIAYCERLEILTRQKHPLGDVLRFDDENAMLMTYYRNNVIHMFALL
                     ALLACLVQNNRRLTRDTLIRLTQQLYPFMQTELFIHWQEAELPELVHRCGDLLVEAGY
                     LQGNDSEVWAPDARSTESMHLSILANTVKQSLERFYITIEILLKSGSGVLRRAQLEEA
                     CTLLAQRMALLHEFSGPEFSDKALFKNFIQALLKNGMLWIDDNGLLAYGDPLRQSHED
                     AHSLLGADTRQSIQQIAELDLEKEMPIQPEKPVKEKKEKERN"
     gene            complement(1498..2277)
                     /locus_tag="VFX11_17990"
     CDS             complement(1498..2277)
                     /locus_tag="VFX11_17990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940569.1"
                     /translation="MTESVPAWIALDRPALRQRLQQLGIAPDVLQGHWYADVPDTANL
                     AAILEWHDDGIQLRATDRELGNPIRVDFLHGKTGFRSQRFQHEMIVKAVTGRSRDPLS
                     VLDCTAGLGRDGFLLAAAGFSVTLVERHPAIACLLLDGLKRAAEDRDAAPVCARIQTH
                     VGSAHDYLAQLEPAAWPDVICVDPMFPERGKTALVKKEMRLFRAVVGDDEDAEALLAL
                     ALQRARKRVVVKRPRKAEFIGGRKPGHQLEGTSSRFDVYVV"
     gene            complement(2329..2703)
                     /locus_tag="VFX11_17995"
     CDS             complement(2329..2703)
                     /locus_tag="VFX11_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940570.1"
                     /translation="MKIGGSQFQSQIGSTGYAREWQRQDVQQAQREQEKARERVVRGD
                     TIDADVQISDGARRSAQQRQVLAGSGAASTQRSPQYYPLPDRGSLSASQQRALQTYST
                     NQGLSRTIDARSEFLGSVDVFA"
BASE COUNT          600 a          899 c          816 g          541 t
ORIGIN      
        1 cgccgtggtg gcgccttctt catgcgtcgc agcttcaagg gcaaccgcct ttattccgcc
       61 gttttcaacg aatacctcaa catggtgttc gaccggggct tctcggtgga atatttcatc
      121 gaaggtggac gcagccgtac cggccgcctg ctggagcccc gcgccggcat gctcaacatg
      181 accgtccgca gcttcctgcg cacacggcgc cgttccttcg tctttattcc cgtgcatttc
      241 agctatgaaa aggtgctgga aggcggcacc tatctgggcg agctgcacgg caaggccaag
      301 aagaaggaaa gcgtgttcga catcttccgc accctgcgca agatcaaggg caccttcggt
      361 caggtgcacg tcaacgtggg cgagccgatt cacctgaacc agttgctggg ggatatcgcc
      421 cccggctggc gcagcgaagc ctacgacgac aacgaaccgc cgtcatggct gaacgattcc
      481 gtcaatgaac tgggtaatcg catcatcacg cacatcaatg atgccgctgt ggtgaacccg
      541 gtgaacctgc tgtgcctgac cctgctgtcg actaccaagc tggccatgga cgaaaaggcc
      601 ctggccaagc agctggattt ctacctgacc ttcctgcgcc ggattccgta ttccggcatc
      661 gcggtagtac ccagcctctc ggggtctgac atcattgcct attgtgaacg gctggaaatc
      721 ctgacgcggc agaagcatcc gctgggcgac gtgctgcgtt tcgacgacga aaacgccatg
      781 ctgatgacgt actaccgcaa caacgtcatc cacatgtttg cgctgctggc cctgctggcc
      841 tgcctggtgc agaacaaccg ccgcctgacg cgtgacaccc tgatccggct gacccagcaa
      901 ctctacccct tcatgcagac cgaactgttt attcactggc aggaagcgga gctgccggaa
      961 ctggtgcacc gttgcgggga cctgctggtg gaggccggtt acctgcaggg caacgacagt
     1021 gaggtgtggg cgccggacgc ccgcagcacc gaaagcatgc atctgtcgat cctggccaac
     1081 acagtgaagc aatcgctgga acgcttctac atcaccatcg agatcctgct gaagtccggt
     1141 tccggcgtgc tgcgacgggc gcaactggaa gaagcctgca ccctgctggc ccagcgcatg
     1201 gcattgctgc atgaattcag cggcccggaa ttctctgaca aggcactgtt caagaatttc
     1261 atccaggcac tgctgaaaaa cgggatgctg tggatcgatg acaatggcct gctggcctat
     1321 ggtgatccat tgcgacagtc gcacgaagac gcgcactccc tgctgggtgc agacacacgg
     1381 cagagcatcc agcagatcgc cgagctggat ctggaaaaag aaatgccgat ccaacccgag
     1441 aagccggtaa aagagaagaa agagaaagaa cgtaattgag aaccggaccg gcggccgcta
     1501 cacgacgtac acgtcaaagc ggctgctggt tccctccagt tgatggccgg gtttgcgtcc
     1561 gccgatgaat tccgccttgc gcggacgctt caccaccacc cgcttgcggg cccgctgcaa
     1621 cgccagcgcc agcaacgcct cggcatcctc gtcatccccc accaccgcgc ggaacagccg
     1681 catttccttt ttcaccaagg cggttttgcc acgctcgggg aacatgggat cgacacagat
     1741 cacatccggc caggccgccg gctcgagttg cgccagataa tcgtgggcac tgcccacgtg
     1801 agtttgaatc cgcgcgcaca ccggcgcggc atcccgatcc tccgccgcgc gttttaaacc
     1861 gtccagcaac aggcaggcga tcgctggatg ccgctccacc agcgtgacgg aaaatcccgc
     1921 cgccgccagc agaaagccat cacgccccaa accggcggtg caatccagca cgctgagcgg
     1981 atcgcggctg cgtcccgtca cggccttcac gatcatttca tgctgaaagc gctgactgcg
     2041 gaatccggtt ttgccgtgca ggaaatccac ccggatcgga ttacccaatt cccggtcggt
     2101 cgcccgcagc tgaatcccgt cgtcgtgcca ttccagtatc gccgccagat tcgccgtatc
     2161 gggaacgtcg gcataccagt gcccctgcag cacgtcaggc gcaataccca gttgctgcaa
     2221 acgctggcgc aaggccggcc ggtccagggc aatccaggcg ggaaccgatt cagtcatggg
     2281 ggaattccgg atatggacag aaaaacgtgc agcctgttac tcagactatc aggcaaacac
     2341 atccacagag ccaaggaatt cactcctggc gtcgatggtg cggctgaggc cctggttggt
     2401 gctgtaggtt tgcaatgcgc gctgctgact ggcggacaga ctgccacgat ctggcagcgg
     2461 ataatattgg ggactgcgtt gtgtggaggc tgcaccactg ccggccagca cctgacgctg
     2521 ctgcgcggag cggcgggcgc catcgctgat ctggacatcg gcgtcgatcg tgtcgccacg
     2581 gacgactcgc tcgcgcgcct tttcctgctc gcgctgagcc tgctgcacgt cctgccgctg
     2641 ccattcgcgg gcataacctg tgctgccaat ctgtgactga aactgggatc caccaatctt
     2701 catagaaccg gtctgactgc cttcaatact gccaatgcat agggttgtgg agatctgacg
     2761 cacgaacaga tgttgaatca gtatagctgc caccggcgca ggccggcgcc taatcagctt
     2821 ctgttcaatg atccacaaat aaattcaacg ggatag
//
LOCUS       DASUEG010000955         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1394909, whole genome shotgun sequence.
ACCESSION   DASUEG010000955 DASUEG010000000
VERSION     DASUEG010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394909"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2583
                     /locus_tag="VFX11_18000"
     CDS             <1..>2583
                     /locus_tag="VFX11_18000"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HEX4940571.1"
                     /translation="DIPWRLHTELSVPLLQVMAVVLTSVLAQAALWAQTYLVTGFLMD
                     ALEGRAPTLDRAREHLKQGAYRGAVYGGLFMILVHLLAGGYHSLAALIVAFPYVSGAL
                     LGMLLFPLAKTIVESFDGSAPFVARLLTSLRDPLNYVRGLVIGSGIVLALDQQLPGHG
                     AWQRSGYGFALGAIAYSGVHLARDWLRIARGQRQFLQGWKVYAVEMLLGGVVGAALTW
                     YGETTQLQVILEKFARYATLSVANPEPYIIYPLFSKWGALNLGLETGGGRLLFNESLS
                     GVISWSMAAPLFSLNLVFLTALLQRSTRPIREFTTTVGLKKVIAEALRVERWGLWMAP
                     IIYSFLRMAPDPAWYNQDGAVRTLVATGMSLAMDDTGFRTWSLNLFMSLLAFDWLRIL
                     IWFDHMGLRVATLVNLSFIGGDMLDEKTARAVGYPSATRYIPEGLRRFGTWMPLLLPF
                     YIPRGQEWDTAWNTAEQMALQAQAEAISASAWLCAGFACLGVLVAAVRLRKGGGSHQR
                     YGGWATQIFKLSNERYALELDPQGRGFSLAQRSAGNREQVDLTQRPLSTLSATGKIFY
                     LLENSADSPADGGLPEIWSLHSSPRPAPGAQNGEHPQRKSLGYSCLHRDLRAHAKVSV
                     HRNDALERWDITLTNTGSRERVVQLISFRDLMLRANGAADRHQSFNDLHIATAFVPPL
                     QALFASNRLLTGFNKQPAGETYFHALAPLPQGTRLLGFQDTRSGILGAGSKRFPATLF
                     HREPLLLEAGTLHQFDPCASLGVEVTLAPSAHITITFVEGWATSRQDAMNLLHRHLKT
                     PVLSAEQLTQALNQPRQHRPELPCPQPAGQETPCSPWHFSPDGRSLQVGSNTPRPWSH
                     PMANA"
BASE COUNT          434 a          891 c          808 g          450 t
ORIGIN      
        1 gatattccct ggcgtttgca tactgaactg tcggtgccgc tgctgcaggt aatggccgtg
       61 gtgctgacat cggtgctggc gcaggcggcg ctgtgggcac aaacctatct ggtgaccggt
      121 ttcctgatgg atgcgctgga aggccgcgca cccacgctgg accgcgcccg tgaacacctg
      181 aaacagggcg cctatcgcgg cgcagtgtac ggtggcctgt tcatgattct ggtgcacctg
      241 ctggcgggcg gctatcacag cctggcggcg ctgatcgtgg cattcccgta tgtaagcggc
      301 gcgctgctgg gcatgctgct gtttccgctg gcgaaaacca tcgtggaaag tttcgacggc
      361 agtgcgccgt tcgtggcgcg actgctgacc agcctgcgtg atccactgaa ttatgtgcgg
      421 ggcctggtga ttggcagcgg catcgtgctg gcgctggacc agcagctgcc ggggcacggc
      481 gcgtggcagc gttccggcta cggttttgca ctgggcgcga tcgcctacag tggcgtgcat
      541 ctggcgcgcg actggctgcg catcgcccgc ggtcagcgcc agtttctgca gggctggaaa
      601 gtctacgccg tggaaatgct gctgggcggc gtggtcggcg cggcgctgac ctggtacggc
      661 gaaaccaccc agctgcaggt gatcctggaa aaattcgcgc gctacgccac cttgagcgtg
      721 gccaaccccg agccgtacat catctatccg ctgttcagca aatggggcgc actgaatctg
      781 ggtctggaaa ccggcggcgg ccggctgctg ttcaacgaat ccctgtccgg cgtgatcagc
      841 tggtcgatgg cggcgccgct gttcagtctc aacctggtat tcctcaccgc gctgctgcag
      901 cgcagcaccc gcccgatccg cgaattcacc accacggtgg gcctgaaaaa agtgatcgcc
      961 gaagcactga gagtcgagcg ctggggcctg tggatggcgc cgatcatcta ttcgttcctg
     1021 cgcatggcgc ccgatccggc ctggtacaac caggacggcg ccgtgcgcac gctggtggcg
     1081 accggcatgt cgctggcgat ggacgacacc ggcttccgta cctggagtct caacctgttc
     1141 atgagtctgc tggcgttcga ctggctgcgc atcctgatct ggttcgatca catgggcctg
     1201 cgcgtggcca cgctggtgaa cctgtccttc atcggcggcg acatgctgga cgagaaaacc
     1261 gcccgcgccg tgggctaccc gtccgctacc cgctacattc ccgaagggct gcgccgcttc
     1321 ggcacctgga tgcccttgct gctgccgttc tacattccgc gcggccagga atgggacacc
     1381 gcctggaaca ccgcagaaca gatggcgttg caggcccagg ccgaagccat ctccgccagc
     1441 gcctggctgt gcgccggttt tgcctgcctg ggcgtgctgg tggcggcagt acggctgcgc
     1501 aagggtggcg gttcgcacca gcgctatggc ggctgggcca cacagatttt caaactgagc
     1561 aatgaacgct acgcactgga actggacccg cagggacgtg gcttcagcct ggcccagcgc
     1621 agtgccggca accgcgagca ggtggacctg acccagcgcc cgctgtccac cctgtccgcc
     1681 accggcaaga ttttctatct gctggaaaac agcgccgatt cgcccgctga cggcggcctg
     1741 cccgagatct ggtcgctgca ttcctcgccc cgcccggccc ccggcgcaca aaacggcgag
     1801 catccgcagc gtaaatccct gggttattcc tgcctgcacc gggatctgcg ggcccacgcc
     1861 aaagtcagcg tacaccgcaa cgacgcgctg gaacgctggg acatcaccct caccaacacc
     1921 ggcagccgcg aacgggtggt gcaactgatc agcttccgcg atctcatgct gcgcgccaac
     1981 ggcgccgccg accgccacca gagcttcaac gacctgcaca tcgccaccgc gttcgtgccg
     2041 ccgctgcagg ccctgttcgc cagcaaccgg ctgctcacgg gcttcaacaa gcagccagca
     2101 ggggaaacct atttccatgc cctggcgccc ctgccgcagg gtacccgtct gctgggtttc
     2161 caggacaccc gctccggtat cctcggcgcc ggttccaaac gtttcccggc caccctgttc
     2221 caccgtgagc cgctgctgct ggaagccggc accctgcacc agttcgaccc ctgcgccagc
     2281 ctgggcgtgg aggtgacgct ggcccccagc gcccacatca cgatcacctt tgtggaaggc
     2341 tgggccacca gtcgccagga cgcaatgaac ctgctgcacc gccacctgaa aacaccggtg
     2401 ctgagtgcgg aacaactgac gcaagccctg aaccagccgc gccagcaccg gccggaactg
     2461 ccctgcccgc aaccggccgg acaggaaacc ccgtgcagcc cctggcattt cagccccgac
     2521 ggccgcagcc tgcaggtggg cagcaacacc ccccgcccct ggtcgcatcc catggccaac
     2581 gca
//
LOCUS       DASUEG010000956         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_30951, whole genome shotgun sequence.
ACCESSION   DASUEG010000956 DASUEG010000000
VERSION     DASUEG010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30951"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /locus_tag="VFX11_18005"
     CDS             complement(<1..2509)
                     /locus_tag="VFX11_18005"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX4940572.1"
                     /translation="MVNLSLGEDYTLLIERLTSLVQERFERTHSDQTPGVVEFCRHYY
                     AAAPFHELQRKRIEDLYGMSVGLWDFIRVMPDKSPRIRVFNPRFEDHGWQSTHTVVEV
                     LAKDSAFLVDTVMMEVVRHGLSIHSVMTNVFWAQRNERGELQNFSFGHKGDGQREALI
                     HVEFDRQPDAQLRAEFARPLQDTLHELSLAVQDFPAMQNHTIAIAEQLKALQPAPVEA
                     SLAEVKHFLSWLVRNNFTFLAMQEYTLVNRDGAASLEKIDGSSMGVARVRQDLELLEA
                     QGGPLLQPEKLIVFGKSGSRSRWHRPVYMDFIGVRKCNDAGEIIGEYRWLGLYTSLVF
                     NNSPRDFPIIGHKLRKVLEGSGLEPTGHDGKRLMQILETFPREELFQTSTEDLLKTAV
                     GVLHIQERRRLRMFVRRSEFSRFLAFLIYTPRDVYDTALRKRFQTVLHEYLDVEDLEF
                     NTYFTESTLTRLYIVVKVNRDSPTEFDVRELEDRLVELARNWSDRLYETLIESAGDDH
                     AGALFTRYADAFSVSYREEFSTRTAVSDIEHIESLDQRAPLAVSFYRNLEDEPHALRF
                     KLFRLEQDVPLSDVLPMLENLGLRVLAGRPYRIELKDASTVWIYDFSVRYDSDQVIDL
                     EKVKQKFQDAFIQTWNGAAEDDSFNRLVLSVGLEWREVAMLRAYAKYFKQTGFAFSQT
                     YIKDALVNHPRVTRLLVDLFELRFQLTGGHSDSSEAALRNRIVECLEQVNSLDEDRIL
                     RRYLDMITGTLRTNFYQKNAEGEFKSYISLKISPRDIPDLPKPLPMFEIFVYSPRVEG
                     VHLRGGKVARGGLRWSDRREDFRTEVMGLVKAQQVKNA"
     gene            complement(2601..>3394)
                     /locus_tag="VFX11_18010"
     CDS             complement(2601..>3394)
                     /locus_tag="VFX11_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4940573.1"
                     /translation="DSLEVVYGGRLDDYSDFDQQFSPRLGLLHTTHDQAVLKILYGHA
                     YRAPNLSEMFGSEQLQPNFDLEAEEMDSLELVYQKQTRHWFSTLTLFRNWWSEAIRGA
                     QVEEPMEGFLYQFRNTGRNEAQGVEAETQARWEQARLSLAASYVRSENVDSGEVFSAF
                     PEWMLDVGWGYRFSAQWDVYINNRLLLRDAASAPVIPGDPIEPADDYFRTDLEVAWSP
                     TDALITRAAVRNLFDRTNYLPSFLYHESGIPDNGVGLTLALELQL"
BASE COUNT          678 a         1042 c          977 g          697 t
ORIGIN      
        1 ctgcgttttt tacctgttgc gcctttacca gccccatgac ttcggtgcgg aaatcctcac
       61 gccggtccga ccagcgcagg ccaccccgcg ccaccttgcc gccgcgcaga tgcacgcctt
      121 ccacccgggg tgaatagacg aagatttcaa acatcggcag cggcttgggc aggtccggaa
      181 tatcgcgcgg agaaatcttc agcgagatat aggatttgaa ttcgccctcg gcatttttct
      241 ggtagaagtt cgtgcgcagc gtgccggtga tcatgtccag gtaacgccgc aggatgcgat
      301 cctcgtccag gctgttcacc tgctccagac attcgacgat gcgattgcgc agggccgcct
      361 cgctgctgtc actgtgtcca cccgtcaact ggaagcgcag ctcgaacaga tccaccagca
      421 gccgggtgac gcggggatga ttcaccagcg cgtccttgat ataagtctgg ctgaaggcaa
      481 acccggtctg cttgaaatac ttggcgtagg cccgcagcat cgccacctcg cgccactcca
      541 gtcccaccga cagcaccagc cggttgaagc tgtcgtcctc cgccgcaccg ttccaggttt
      601 gaatgaaagc gtcctggaat ttctgtttca ccttttccag atcgatcacc tgatcgctgt
      661 cataacgcac gctgaaatcg tagatccaga ccgtgctcgc gtccttcagc tcgatccggt
      721 aaggccgccc cgccagcacc cgcagcccca ggttttccag catcggcagc acatccgaca
      781 gcggtacgtc ctgctccagc cggaacagtt tgaaccgcag ggcgtggggc tcgtcctcca
      841 gattgcggta gaaggacacg gccagcggtg cgcgctgatc caggctttcg atatgctcga
      901 tatcggacac ggccgtgcgg gtggaaaatt cctcgcggta gctcaccgaa aaggcatcgg
      961 catagcgggt gaacagcgcc ccggcatgat catcgccggc ggattcgatc agggtttcgt
     1021 acaggcggtc ggaccagttg cgggccagtt ccaccagccg gtcttccagt tcgcgcacat
     1081 cgaattccgt gggcgaatcc cggttcacct tcaccacgat gtacaggcgc gtcagggtgg
     1141 attcggtgaa ataggtattg aactccagat cctccacgtc caggtattca tgcagcacgg
     1201 tctggaagcg tttgcgcagc gcggtgtcgt acacatcgcg gggcgtgtaa atcaggaatg
     1261 ccaggaaacg gctgaattcg ctgcgccgaa cgaacatgcg caaacgccgc cgctcctgaa
     1321 tgtgcagcac gcccacggcg gttttcagca gatcctcggt ggacgtctgg aacagttcct
     1381 cgcggggaaa ggtttccaga atctgcatca ggcgcttgcc gtcgtggccg gtgggctcca
     1441 gtccggagcc ttccagcact ttccgcagtt tgtgaccgat gatgggaaaa tcccgcggcg
     1501 agttgttgaa caccaacgag gtgtagagcc ccagccagcg gtattcgcca atgatttcgc
     1561 cggcatcgtt gcacttgcgc acgccgatga aatccatgta cacggggcga tgccagcggg
     1621 aacgggagcc ggacttgccg aacacgatca gtttttccgg ctgcagcaac ggcccgccct
     1681 gcgcttccag cagctccagg tcttggcgca cccgtgccac gcccatgctg ctgccgtcga
     1741 ttttttccag cgacgctgcg ccatctctgt ttaccagggt gtattcctgc atcgccagaa
     1801 aggtgaaatt gttgcgcacc agccagctca ggaaatgctt cacctccgcc agcgacgctt
     1861 ccaccggcgc cggctgcagc gccttgagct gttcggcaat ggcaatggtg tgattctgca
     1921 tggcgggaaa atcctgcacc gccagcgaca gttcgtgcag cgtgtcctgc agtggccgcg
     1981 caaactcggc gcgcagctgg gcgtcaggct ggcggtcgaa ttccacatgg atcagcgcct
     2041 cgcgctgacc atcgcccttg tggccgaaac tgaaattctg caattcgccg cgttcgttgc
     2101 gctgggccca gaacacgttg gtcattactg aatgtatcga caggccatgt cgcaccactt
     2161 ccatcatcac ggtgtccacc aggaaggcgc tgtccttggc cagcacttcc actacggtgt
     2221 gggtgctctg ccagccgtgg tcttcgaagc ggggattgaa tacgcggatg cgggggctct
     2281 tgtcgggcat gacgcggatg aaatcccaca gccccaccga catgccatag agatcctcga
     2341 tgcgcttgcg ttgcagttca tggaacggtg cggccgcata gtagtggcgg cagaattcga
     2401 ctacgcccgg ggtctgatcc gagtgggtac gctcaaaccg ctcctgcacc agcgaagtca
     2461 gacgctcgat cagcagggtg tagtcttcac ccagcgacag attgaccatt tcttttcccc
     2521 tcggcccttg tccatttcaa tctgatcagt ttagtacaga cagggcggga ttggttccgc
     2581 gcagagtcaa ggaatggctg tcagagctgc agttccagcg ccagcgtcag gccaacgccg
     2641 ttatccggga tgccggattc atgatagagg aaggatggca ggtaattggt gcggtcaaac
     2701 aggttgcgca ccgcagcgcg ggtgatcaac gcgtccgtgg gtgaccaggc cacttcgaga
     2761 tccgtacgga aatagtcgtc ggccggttcg atgggatctc ccggaatgac aggggcagac
     2821 gccgcgtcgc gcagcagcag acggttgttg atatagacat cccactgcgc gctgaaacga
     2881 tacccccacc cgacatccag catccattcc ggaaacgcgg aaaacacttc accgctgtcg
     2941 acgttttcgc tgcgcacata ggatgccgcc aggctcaggc gtgcctgctc ccagcgggcc
     3001 tgggtttcag cttccacacc ctgggcctcg ttacgccccg tgttgcggaa ctgatacaga
     3061 aaaccctcca tcggctcttc cacttgagca ccgcgaatcg cttcactcca ccagttccgg
     3121 aacagagtga gtgttgagaa ccagtgccgg gtctgcttct ggtagaccag ctccagactg
     3181 tccatctctt ccgcttccag atcaaagttg ggttgcaatt gctcgctgcc aaacatctcg
     3241 gacaggttgg gtgcacggta ggcgtgacca tacaggattt tcagtaccgc ctgatcatgg
     3301 gtggtgtgca aaaggcccag acggggactg aattgctgat caaaatcgct gtagtcatcg
     3361 agacggccac cgtacaccac ttccaatgag tccc
//
LOCUS       DASUEG010000957         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_676608, whole genome shotgun sequence.
ACCESSION   DASUEG010000957 DASUEG010000000
VERSION     DASUEG010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676608"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..417)
                     /locus_tag="VFX11_18015"
     CDS             complement(67..417)
                     /locus_tag="VFX11_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940574.1"
                     /translation="MTPDHDELPRRLSQSLDRSLDSLDAETQQALQQIRRDALQPRRR
                     NLRPAMMIAASLVALVIVPWLALRQPEVAPDMATDMTTDTFDLAAENSYLSEDPDLLA
                     DWEMLDAIGEVPDA"
     gene            complement(414..1007)
                     /locus_tag="VFX11_18020"
     CDS             complement(414..1007)
                     /locus_tag="VFX11_18020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4940575.1"
                     /translation="MLDCSDPRFGHHAMTQTVTPLSLDGFLASVERRAYRMALLATRQ
                     PADALDIVQDAMLKLVDRYRHAPHAEWPLLFQRILQNRILDWHRRQTRSKRWFVFAAP
                     VNEDEAAEKDALDDIADEREQDPPELLARANDMATVLKVVEALPLRQQQAFLLRAWEG
                     LDVSATATAMDCSEGAVKSHYFRALQTLRAALQPEAP"
     gene            complement(1031..1570)
                     /locus_tag="VFX11_18025"
     CDS             complement(1031..1570)
                     /locus_tag="VFX11_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940576.1"
                     /translation="MKIHHLMLAVALTASGVVPVAHAERAAKHPRHSYSADVTRTYGN
                     GKTVNRHTEQTATATGFNRSSTMTGSDGRTASRNVSANRDAEAGTYTKSINGTRMNGD
                     TYSGERVTQKTDDGYTRSSTRTNASGETASKQVSAVVDRENGTLTKNISATGFNGETH
                     SATVVKTYSKGDATGSAQN"
     gene            1753..2553
                     /locus_tag="VFX11_18030"
     CDS             1753..2553
                     /locus_tag="VFX11_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX4940577.1"
                     /translation="MSERVRVQVDGTVAYVTLNRADKHNGMDMEMLKAVIAAQKQVRK
                     LKDIRAVIIHGDGPSFCAGLDFKSVLGQPMKALTSYLQLWWPFRNNFQTWSIGWREVP
                     VPVIAVMHGNCFGAGLQLGLGADIRICTPDAKLSMMEAKWGLVPDMGGVALMRELMPI
                     DVAKEITMTGRVINGVDAKALGLVTHVSDDPLTHAKNLVDEISVRSPDAVGAGKFLLQ
                     EAWRALDGAALRAERLWQRRVMGTKNQRISVERNQKKQDIPFRDRTLS"
     gene            2746..>3732
                     /locus_tag="VFX11_18035"
     CDS             2746..>3732
                     /locus_tag="VFX11_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4940578.1"
                     /translation="MNAATQTQARADWASQAVSRQRAFFESGATRSYEFRLQQLKQLK
                     AAMLKYADEVEAALKADIGRPPLEAYVEMSTAMEELNHTLKHLKKWMQPKKVGTPLLA
                     QPGRSRIEYTSQGVVFIMGPYNYPFVLCIQPLIGALAAGNTVIMKPSSLNPQTANVME
                     KMAKEFFSPDVFQIFLGSTEVTDALLEEQFDHIFFTGSPRVGKIVMAKAAKYLTPVTL
                     ELGGKSPTIVHSDAKLDVAARRILAGKFMNVGQTCIAPDHLFVHASIKDEFERKLVQT
                     LKDFYGNEPQKSPDLGRMINSRHFERVKALIDPAKVLAGGQTDAKELYIAPTL"
BASE COUNT          690 a         1086 c         1230 g          726 t
ORIGIN      
        1 ggtggggcgt cggcatgggc gacagcggcc accagcagga gcggccagaa cagccggcga
       61 cgggaatcag gcatcgggca cctcgccgat ggcatccagc atttcccagt ccgccagcag
      121 gtccgggtct tcgctcaggt agctgttttc cgctgccaga tcgaatgtgt ccgttgtcat
      181 atcagttgcc atgtcaggtg ccacttccgg ttgccgcagc gcgagccagg gcacgatgac
      241 cagagccacc aggctggcag cgatcatcat ggccgggcgc aggttgcgcc gccgcggttg
      301 cagtgcgtcg cggcgaatct gctgcagtgc ctgctgcgtt tccgcatcga gcgaatccag
      361 gctgcgatcc agtgactggc tcaggcggcg gggcagttcg tcatggtccg gtgtcatggg
      421 gcctccggtt gcaaggcggc gcgcagggtt tgcagcgcac ggaaataatg ggatttcacg
      481 gcgccttcgc tgcaatccat cgcagtggcc gtggcgctca cgtcgagccc ttcccaggcc
      541 cgcagcagaa acgcctgttg ctggcgcagt ggcagcgctt ccaccacttt caatacggtg
      601 gccatgtcgt tggcgcgggc cagcagttcc ggcgggtcct gttcgcgttc gtcggcgatg
      661 tcgtccagcg catccttttc tgccgcttca tcctcattca ccggtgccgc aaaaacaaac
      721 cagcgtttgc tgcgggtctg gcgccggtgc cagtcgagaa tacggttttg caggatgcgc
      781 tggaacagca gcggccattc ggcgtggggc gcgtgacgat aacgatccac cagtttcagc
      841 atcgcatcct gcacgatgtc gagcgcatcg gcgggctggc gcgtggcgag caacgccatg
      901 cggtaggcac ggcgttccac gctggcaaga aagccgtcga ggcttaacgg tgtgacagtc
      961 tgcgtcatgg cgtgatggcc gaacctggga tcactgcagt ctagcattgt ccgcttccgc
     1021 gcccttgccc tcagttctgc gccgacccgg tagcgtcacc cttgctgtag gttttcacca
     1081 cggtggcgct gtgcgtttca ccattgaaac cggtcgcgct gatgttcttg gtgagcgtgc
     1141 cgttttccct gtccacgacg gcgctcacct gcttgctggc ggtttcgccg ctggcattgg
     1201 tgcgggtgct gctgcgggtg taaccgtcgt cggttttctg ggtgacgcgc tcgccggaat
     1261 aggtatcgcc attcatgcgg gtgccgttga tgcttttggt gtaggtgccg gcttcggcat
     1321 cgcggttggc gctgacgttg cggctggcgg ttctgccgtc ggagccggtc atggtgctgc
     1381 tgcggttgaa gccggtggcg gtggccgtct gctcggtgtg gcggttgacg gttttgccgt
     1441 tgccataagt gcgggtcacg tcagcgctgt agctgtggcg tgggtgtttg gccgcgcgct
     1501 cggcatgggc gaccggtacg acaccgcttg cggtcagggc aacagccagc atcaaatggt
     1561 ggattttcat gggtttactc cttcaaagca cggttggggg tggtggcttc cggcagcggt
     1621 ttgcgcggcc tctgtgacgt ttaacgcgcg acggcggaat cggtttacac gccggatgaa
     1681 aaattttttc ccagcggagc ggcagcggtg tagccttccg tcatcttttt ttgatgaagc
     1741 aggagtgttg ccatgagtga gcgggttcgc gtccaggtgg acggcacggt ggcctatgtc
     1801 acccttaacc gggccgacaa gcacaacggc atggacatgg aaatgctgaa agcagtgatc
     1861 gcggcgcaga agcaggtgcg caagctgaag gacatccgcg cagtgatcat tcacggcgac
     1921 gggccgtcgt tctgcgccgg gctggatttc aaaagcgtgc tggggcagcc gatgaaggcg
     1981 ctcaccagct atctgcaatt gtggtggccg ttccgcaata actttcagac ctggagcatt
     2041 ggctggcgcg aagtgccggt accggtgatt gcggtgatgc acggcaactg ttttggcgca
     2101 gggctgcagc tgggcctggg tgccgacatc cgcatctgca cgccggacgc aaaactgtcg
     2161 atgatggaag cgaaatgggg tctggtgccg gacatgggcg gcgtggcgct gatgcgcgaa
     2221 ctgatgccga tcgacgtggc gaaggaaatc accatgaccg gccgcgtgat caatggtgtt
     2281 gacgcgaagg cgctgggcct ggtgacccac gtgagtgacg atccgttaac gcacgccaaa
     2341 aatctggtgg acgaaatttc cgtgcgctcg ccggacgccg tgggtgccgg caaattcctg
     2401 ctgcaggaag cctggcgtgc gctggacggt gctgcactgc gggcggaacg gttgtggcag
     2461 cggcgggtga tgggcacgaa aaaccagcgc atttcggtgg agcggaacca gaagaagcag
     2521 gatattccgt tcagggacag aacactgtcc tgagccagcg ccggctgact ggctgccggc
     2581 ctggattcgt tcctgaacag gaaacgatcg ggcattggtg ggccagcccg gcaccggtcc
     2641 agcccgcgct gaccgttttg gtcacccaac ttgagcaccc cggcaattgc ggtgacgcgc
     2701 cagtcatgat tggatcacgc taccaaaaac aaggagcgga cccccatgaa tgcagccacc
     2761 caaacccaag cccgtgccga ttgggccagt caagccgtct cccgccagcg ggcctttttc
     2821 gaatccggcg ccacccgcag ttacgagttc cgcctgcagc agctcaagca gctgaaagcc
     2881 gccatgctga agtacgccga cgaggtggaa gccgcactga aggccgacat cggccgtccg
     2941 ccgctggaag cctacgtgga aatgagcacg gcgatggagg agttgaacca caccctcaag
     3001 cacctgaaaa agtggatgca gcccaagaaa gtgggcacgc cgctgctggc ccagcccggc
     3061 cgcagccgga ttgagtacac gtcccagggc gtggtgttca tcatggggcc ctacaactac
     3121 ccgtttgtgc tgtgcatcca gcccctgatc ggcgcgctgg cggcgggcaa cacggtgatc
     3181 atgaaaccct cgtcgctgaa tccgcagacc gccaatgtca tggagaaaat ggcgaaggaa
     3241 ttcttctcgc cggatgtgtt ccagattttc ctgggcagta ccgaagtcac tgacgccctg
     3301 ctggaagagc agttcgacca tatcttcttc accggcagcc cgcgcgttgg caagatcgtg
     3361 atggcgaaag cggcaaaata cctgacgccc gtaacgctgg aactgggcgg caagagcccc
     3421 accatcgtgc acagcgacgc caaactggat gtggccgcca ggcgcattct ggcaggcaag
     3481 ttcatgaacg tgggccagac ctgcatcgcg ccggatcacc tgttcgtgca cgcgtccata
     3541 aaggatgagt tcgaacgcaa gctggtgcag acgctgaagg atttctacgg caatgaaccc
     3601 cagaaaagcc cggatctggg ccgcatgatc aacagcaggc atttcgaacg ggtgaaagcc
     3661 ctgatcgatc ccgccaaggt actggccggc ggccagaccg atgcaaagga gctgtacatc
     3721 gcacccacgc tg
//
LOCUS       DASUEG010000958         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_411666, whole genome shotgun sequence.
ACCESSION   DASUEG010000958 DASUEG010000000
VERSION     DASUEG010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411666"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..659)
                     /gene="pdxH"
                     /locus_tag="VFX11_18040"
     CDS             complement(15..659)
                     /gene="pdxH"
                     /locus_tag="VFX11_18040"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX4940579.1"
                     /translation="MTTRDDLTHIRNDYARARLGPEDCLPDPVAQFSRWLDEAITSKV
                     NEPTAMNVATVENGRPSARMVLLKGIEQGTFVFYTNYESRKGRQIEANPFVALTLFWP
                     ELERQVRVEGQATRVASATSDAYFASRPYQSRVGAWASEQSRPLDSTASLVARAARFA
                     AQYVTGVPRPPHWGGYAVLPERMEFWQGRPSRLHDRVLYLRDANGHWVRERLYP"
     gene            913..1425
                     /locus_tag="VFX11_18045"
     CDS             913..1425
                     /locus_tag="VFX11_18045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4940580.1"
                     /translation="MTLPDDFFTPARPAPGLSVETLLWLRDNDFDPDSLDTPGRYQDT
                     PIILASRRGLLPQVQELISAGVNINHRNMDGTNCLWASIVPNRFDIAEWLLQHGVDID
                     NQNDNGATALMYASSAGKTEWVAFLLTQGANPHLQSLDGYTALDLAGNVECLRLLRKA
                     GLPIATATPA"
     gene            1519..2949
                     /locus_tag="VFX11_18050"
     CDS             1519..2949
                     /locus_tag="VFX11_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4940581.1"
                     /translation="MYSYTDFYINGQWVAPATPRLLDVINPATEQPMGRISLGSAADV
                     DKAVAAAKTAFTTFSQTSREQRIALLEAIIGAFQQRLEDLAQAISAEMGAPLWLARKA
                     QAPSGLAHFMQALEVLKQYDFESRRGTTCLRKEPVGVCALITPWNWPINQIACKVAPA
                     LAAGCTMVLKPSEIAPINAGIFAEIMHAAGVPAGVFNLVNGDGAGVGSALSSHPDIDM
                     VSFTGSTRAGVAIAKNAADTVKRVAQELGGKSANIVLDDADLEAVVSAGVVSCFTNSG
                     QSCNAPTRMLVPASKLAQATAIAKAAADNVKPGDPHAADSTIGPVASAAQFDKIQRLI
                     EAGIEEGAELVAGGPGKPKGLETGYYVRPTVFSNVNNRMTIAREEIFGPVLVIIPYRD
                     DEDAIAIANDTPYGLSGYVSGEAQHARRVAARLRTGTVHLNGAPVDFSAPFGGYKQSG
                     NGREWGREGVEDFLETKAVLGFFNDE"
BASE COUNT          619 a         1073 c          962 g          531 t
ORIGIN      
        1 ggttgatgca gtattcaggg atacaatcgt tcccgcaccc agtgtccgtt ggcgtcgcgc
       61 aaatacaaca cacggtcatg caggcgtgaa ggccggccct gccagaattc catccgctcc
      121 ggcagcaccg cataaccacc ccagtgcggc ggacggggca cacctgtcac atattgcgcg
      181 gcgaagcggg cggcgcgggc gaccaggctg gcggtgctgt ccagcggccg gctctgctcc
      241 gacgcccagg cgcccacccg gctttgatag gggcgggacg caaagtaggc gtcagacgtg
      301 gccgatgcca cccgcgtggc ctgtccctcg acgcgcacct gccgttccag ctccggccag
      361 aacagcgtca gcgccacgaa cggattggcc tcgatctgcc gtcccttgcg gctttcgtaa
      421 ttggtgtaaa acacgaacgt gccctgctcg atgcctttca gcagcaccat gcgtgccgac
      481 ggccgcccgt tttccaccgt agccacgttc atggccgtgg gttcgttcac cttggacgtg
      541 atggcctcat ccagccagcg gctgaactgg gccaccgggt ccggcaggca atcctcgggc
      601 cccaggcgcg cccgggcata gtcgttgcga atgtgggtca ggtcatcgcg ggtcgtcatg
      661 cagaattcca gctggggatt acaactgcag tgtaagcggg gcgtgtcggc aggtcacgag
      721 ggatggtgcc gggcgcctgg attgtcgcct ttacgccgtc gcaaggctga caaatccggc
      781 atcacggctg agggcaatgt cggcagtgaa gccggtttcc gccattttca tgcgtgtgta
      841 ccgaaattca atcggcggca cagtccctgc aatgacgtcg ctgaactgtt tggaatgacc
      901 ggaaccgaat ccatgaccct gcccgatgat ttcttcacac ctgcccgacc cgcgcccggc
      961 ctcagcgtgg aaaccctgct ctggctgcgc gacaacgact tcgatcccga cagccttgac
     1021 acgccgggcc gctaccagga cacgccgatc atcctcgcca gccgccgtgg cctgctgccg
     1081 caggtgcagg aactgatctc cgccggcgtg aacatcaacc accgcaacat ggacggcacc
     1141 aactgtctgt gggcctccat cgtgcccaac cgtttcgaca tcgctgaatg gctgttgcag
     1201 cacggcgtcg acatcgacaa ccagaacgac aacggcgcca ccgcgctgat gtacgcatcc
     1261 tccgccggca agaccgagtg ggtggccttc ctgctgacac agggcgccaa tccgcatctg
     1321 caatccctcg acggctacac cgcgctggat ctggcaggca acgtcgaatg cctgcgcctg
     1381 ctgcgcaagg cgggcttgcc gattgcgaca gcaacaccgg cctgagcaac ccgacccttc
     1441 ccagggtcca tgtgaacttt catactcgca gcagggcgga gcccaccacc gggctcccgt
     1501 tgcgaaggaa agccgcacat gtattcctat accgatttct acatcaacgg ccagtgggta
     1561 gcgccggcaa ctccgcgcct gctggacgtc atcaatcccg ccaccgaaca gcccatgggc
     1621 cgcatcagcc tgggcagcgc ggcggacgtc gacaaggccg tggctgccgc caaaaccgcc
     1681 ttcaccacct tttcgcaaac ctcgcgggaa cagcgcatcg ccctgctgga ggccatcatc
     1741 ggcgccttcc agcagcggct ggaagatctg gcccaggcca tctcggcgga aatgggcgca
     1801 cccctgtggc tggcacgcaa ggcccaggct cccagcgggc tcgcgcattt catgcaggca
     1861 ctggaagtcc tgaagcaata cgactttgaa agccggcgcg gcactacctg cctgcgcaag
     1921 gaaccggtag gcgtgtgcgc tctgatcacg ccctggaact ggcccatcaa ccagatcgcc
     1981 tgcaaggtgg cccccgcatt ggcggcgggc tgcaccatgg tgctcaagcc cagcgagatc
     2041 gcccccatca acgccggcat ctttgcggaa atcatgcacg ccgccggcgt acctgcgggt
     2101 gtattcaacc tggtcaacgg tgatggtgcc ggtgtcggtt ctgcactgag cagccatccc
     2161 gatatcgaca tggtctcttt caccggcagc acccgcgccg gcgtcgccat cgccaaaaac
     2221 gcggccgaca ccgtgaagcg cgtggcgcag gaactgggcg gcaaatccgc caacatcgtg
     2281 ctcgacgacg ccgacctgga agcggtggtg agcgccggcg tggtgagctg cttcaccaac
     2341 agcggacaat cttgcaacgc ccccacccgc atgctggtgc cggcaagcaa actggcacag
     2401 gccaccgcca tcgccaaagc cgcagccgac aacgtcaaac ccggcgatcc gcacgccgcc
     2461 gatagcacga tcggcccggt ggccagcgcc gcgcaattcg acaagatcca gcgtctgatc
     2521 gaagcgggta tcgaagaagg tgccgaactg gtggccggcg gcccgggcaa acccaagggg
     2581 ctggaaaccg gctattacgt cagacccacc gtgttcagca acgtcaacaa ccgcatgacg
     2641 atcgcgcgcg aggaaatctt cggcccggtg ctggtgatca ttccctaccg cgacgacgag
     2701 gacgccatcg ccatcgccaa cgacacgcct tacggattgt ccggctacgt gtcgggtgaa
     2761 gcgcagcacg cgcgccgggt ggcggcacgc ctgcgcacgg gcaccgtcca cctgaacggc
     2821 gcgccggtgg atttcagcgc gccattcggt ggctacaagc agtcgggcaa tggccgcgag
     2881 tggggccgtg aaggcgtcga ggatttcctc gaaaccaaag ccgtgctggg gtttttcaac
     2941 gacgagtagg gttcactgac gaaaaagggc caccaattcg tctagcattg aatcgggcgg
     3001 accggtttat atcgatccgc ccacgcaatt caacccgctg cgttgctcag tgcgtcacca
     3061 cgtactgggc cgcgcactcg gggcaggcgt ccacctgatt gtagagtcgc ttgccgtcgg
     3121 accaggcgct ggcaaaactg gcaatcgaac ccaggtagat tttttccggg atcgtcggca
     3181 acagt
//
LOCUS       DASUEG010000959         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_765070, whole genome shotgun sequence.
ACCESSION   DASUEG010000959 DASUEG010000000
VERSION     DASUEG010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765070"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFX11_18055"
     CDS             <1..442
                     /locus_tag="VFX11_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX4940582.1"
                     /translation="LQLLSRREYSARELQQKLAGGHAAEDLAAVLEQLAEQGLQSDRR
                     FAEIWVRHRQAQGFGPIRIQSELRQKGISPDQIQAAMEADGMDWFAHAGDVWQRRFRG
                     RRERDPRLKAKQIRFLQYRGFTGEQIRHALGSADLELPDADSPD"
     gene            615..>2080
                     /gene="alaS"
                     /locus_tag="VFX11_18060"
     CDS             615..>2080
                     /gene="alaS"
                     /locus_tag="VFX11_18060"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX4940583.1"
                     /translation="MKTSAIRTAFLEYFKTQGHEVVASSSLIPGNDPTLLFTNAGMVQ
                     FKDVFLGRDLRPYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRYAWEFLTSDKWLNLPKEKLWVTVYASDDEAYDIWNKEVGVPAERVIRIGDNKG
                     APYASDNFWQMGDTGPCGPCTEIFYDHGPDIWGGPPGSPEEDGDRYIEIWNNVFMQFD
                     RQPDGTLVPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFASLLKDVARVTDAQDLED
                     KSLRVVADHIRSCAFLIVDGVVPSNEGRGYVLRRILRRAIRHGNKLGARGAFFHKLVA
                     PLVREMGDAYPELKKAQAQVEKMLLQEEEQFAKTLEQGLKILEQDLEGLVGNQIPGDV
                     VFKLYDTYGFPADLTADIARERNLTIDHIGFEKEMDAQKSRSKAAGKFKANYHDGLDV
                     NTRSEFSGYDQVTDSGRITEIFVDGQPVQSVSAGQPAQ"
BASE COUNT          422 a          660 c          626 g          372 t
ORIGIN      
        1 tctgcagttg ttgtcgcgcc gcgaatacag cgcccgagaa ctgcagcaga agctggcggg
       61 cggacatgcg gccgaggatc tggccgccgt gctggaacag ctggcggagc agggcctgca
      121 aagcgatcgt cgttttgccg agatctgggt gcgccatcgt caggcgcagg gttttggccc
      181 catccgcatc cagtccgagc tgcgccagaa aggcatttcc cccgaccaga tccaggctgc
      241 catggaagcg gacgggatgg actggttcgc ccatgccggc gatgtctggc agcgccgttt
      301 tcgcggccgc cgcgagcgtg acccgcgcct gaaagccaaa cagatccgtt tcctgcaata
      361 ccggggcttc accggcgagc agatccgcca tgcactgggt tctgccgatc tggaactgcc
      421 ggacgcagac tcgccggatt aaccgcccgg atcaacggcc cggttcaggg cgccccctgg
      481 aaacgctcct ccgcagccgt ccatctgccc ccatcccgca gggatgaatt gtgccgattt
      541 gatggtaaac tgccgccttt gtcagtgcgc cccagacccg cgctgtcccc cgactttccc
      601 gatccggtac ccgaatgaag acctctgcaa tccgtacagc gtttcttgag tatttcaaga
      661 cccagggcca cgaagtggtg gccagcagct cgctcatccc cggcaacgat cccaccctgc
      721 tgttcaccaa cgccggcatg gtgcagttca aggatgtgtt tctcggccgc gacctgcgtc
      781 cgtacaagcg cgccgtgacc tcgcagcgct gcgtgcgcgc cggcggcaag cacaacgatc
      841 tggaaaacgt aggctacacc gcgcgtcacc ataccttctt cgaaatgctg ggcaatttca
      901 gcttcggcga ttacttcaag cgcgacgcca tccgctatgc gtgggaattc ctgacgtcag
      961 acaaatggtt gaacctgccc aaggaaaaac tctgggtcac cgtatacgcc agtgacgacg
     1021 aagcctacga catctggaac aaggaagtgg gcgtaccagc ggagcgcgtg atccgcatcg
     1081 gcgacaacaa gggcgcgccg tacgcgtccg acaacttctg gcagatgggc gacaccggcc
     1141 cctgtggccc ctgcaccgag attttctacg atcacggccc tgacatctgg ggtggccctc
     1201 ccggttcacc cgaggaagac ggcgatcgct acatcgaaat ctggaacaac gtgttcatgc
     1261 agttcgaccg ccagccggac ggcaccctgg tgccgctgcc caagccgtcg gtggacaccg
     1321 gcatggggtt ggagcggatt gccgccgtca tgcagcacgt gcacagcaat tatgaaatcg
     1381 acctgttcgc cagtctgctg aaagacgtgg cgcgcgttac cgacgcccag gatctggaag
     1441 acaaatccct gcgcgtggtg gccgaccaca tccgctcctg cgcgtttctg atcgtggacg
     1501 gtgtggtgcc gtccaacgaa ggtcgcggct atgtgctgcg ccgcatcctg cgtcgcgcta
     1561 tccgtcatgg caacaagctc ggcgccaggg gcgcgttctt ccacaagctg gtggcaccgc
     1621 tggtgcgtga gatgggcgat gcctatcccg aattgaaaaa ggcacaggcc caggtggaaa
     1681 aaatgctgct gcaggaagaa gagcagttcg cgaaaacgct ggagcagggc ctgaagattc
     1741 tggagcagga tctggaaggt ctggtgggta accagattcc cggcgacgtg gtgttcaagc
     1801 tctacgacac ctacggtttc cctgccgatc ttaccgccga tatcgcccgc gagcgcaatc
     1861 tcaccatcga ccacatcggc ttcgaaaagg aaatggacgc gcagaagtcg cgctccaagg
     1921 ccgccggcaa attcaaggcg aattaccacg acggcctgga cgtgaacacc cgcagcgaat
     1981 tcagcggcta cgaccaggtc accgatagcg gccgcatcac cgagattttt gtcgatggtc
     2041 agccggtgca gtccgtcagt gcgggtcagc cggcccagat
//
LOCUS       DASUEG010000960         6560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1987311, whole genome shotgun sequence.
ACCESSION   DASUEG010000960 DASUEG010000000
VERSION     DASUEG010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987311"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="VFX11_18065"
     CDS             complement(<1..1647)
                     /locus_tag="VFX11_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4940584.1"
                     /translation="MTDSIRLDRRSFISLTLTAGGALLLEFPLTAHAASSTAPPPITG
                     FFEIGTDHQLYILLNRHEMGQGLSDGLRTLFAEELGTAPERIRVRLLSSGPTQANALA
                     QPLNTGGSASTRTSWVPVRQAAAYCRDVLLKAAALTVQAQAPGRPLPRFELRDGWILG
                     PREPLRFAAVLGKVTEAARAVDPKRVRPTLPPWRYIGEWKEPAWLKEKVTGVAKYGFD
                     RACEQVASIERPDRFGAELAGMNEQAVLAVPGVTAVIPLQTGVAVVAKHTWAALQGRK
                     ALAAQWKGGAELSSADLGDTLAGLLDKPGLSQVVVTEGAAPATAPALERVYRLPFLAH
                     TPMEPLACTVSVGSKRCYIQAGTQRPDAVRMLAAGITGLPMKAIQLDDELSGGSFGRR
                     LGTDFIAEALELGVRLRKPVKVVWTREDQLTAGQYRPPAVIRMRGHAGPKGQLAGLQH
                     QVVSTARLNETASVPPAKGDLTQYLKNLMAPAPGKAVEYGQGVVPSKRVGESAVEGVR
                     QCHYRLPYRRVEHVPVDLPVPVGYWRSVGNSLNIFAQEVFI"
     gene            1804..2346
                     /locus_tag="VFX11_18070"
     CDS             1804..2346
                     /locus_tag="VFX11_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
                     /protein_id="HEX4940585.1"
                     /translation="MNSAVPFKDMMQAGASTTEQALAWFDSLDPVDEAFMLGRWRGRG
                     VVTGHPMDGLLELFNWYGKEFLSAEAVHPLLFTASDGSLVKLDPRWMSMKHARNTPLA
                     RHAISRRLFSALSRLMRTEQPRARLRMIEYRGKVSAAMQYDNLPINDIFHRIDDNTVL
                     GVMDLKGMRQPFFFLLEREG"
     gene            complement(2359..4476)
                     /locus_tag="VFX11_18075"
     CDS             complement(2359..4476)
                     /locus_tag="VFX11_18075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4940586.1"
                     /translation="MNGSVLKQGFRPDIQGLRAVAALLVCAYHLWFGRVSGGVDVFFV
                     VSGFLITGMLLRQVDRQGRVDFAGFWARLLKRLLPAAITVILFTSLASYLLLPKVEWK
                     GAIKEAAAAAIYLENWLLAFDAVDYLARETPPSPFQHFWALSTQGQIYLLWPCLVAMV
                     VWLSARLRRDVRATLMTVLAGVFGLSLAYSVYATRMNQAFAYFDTGARLWEFALGSLL
                     AVPRMAWRPQGVAAVVAGWAGLLAVVSCGLLFQVSTVFPGYAALWPTLGALLILLAGS
                     GNNSVWGVGTLLRTRPLQFLGSISYALYLWHWPLLILVYAYSGTREVSLVQGLAILAV
                     SLLLAWSTTRFLERPVRRDWFPGPGPVRAFAFGAGCVTTTALVLAVWAGYTLRLQALE
                     ADAGIRVGSADYPGARALMPGFKADGAVPLPPLPGPFRIKSDLPASYSDASHQQLLDD
                     QVRVGEYGKRDGRYVIALVGGSHSAQWLPALQAVAERQDLRILSMTKSGCWFSDEAKS
                     DQVPEGAGESCRRWNRNLLKELERARPDAVFTTLTRAAGTETVPSKYVEKWWQMQALG
                     IDVIAIRDTPWHAADPALCVDVFGPANRRCQLPRQQSLLPENPAADLAGLPQRLRQID
                     LTGYLCDAERCPPVIGNVMVYRDSNHLSASYAKTLGPALEPFIHDWLASRAPVSDSRN
                     GTLLQAAVDLPAAGVADSAATPH"
     gene            4967..5971
                     /locus_tag="VFX11_18080"
     CDS             4967..5971
                     /locus_tag="VFX11_18080"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX4940587.1"
                     /translation="MSLVSLCKTLFKVHGMALVLTTSGLLLGGCGGGSQSSGSTEPPV
                     QTDGGSTDGSGTQTGGTDPTPTPTPAPEQHSLSLTSQPANAVIYEGQNQTFSLTYSNS
                     HPVSVAWYYNGSPISGASGSSYTVSNATTARAGTYSCAVTDGTLTVTCGSFSLTVHQI
                     VRITQQPINQIVNEGVNANFSVTATGTGPVSYQWYFNDQPLTGATSAQLNLAATSSAN
                     SGQYHVRVSNGGSSANSSKATLTVISNPNGKVVLSWDAPTRRADGSNLAAGDIASYQI
                     FHADSATGALTQLTSVTGAELSLQVEELAIGTHYFALTTVDTQGLVSAQSSRIAVAVQ
                     "
     gene            6290..>6560
                     /locus_tag="VFX11_18085"
     CDS             6290..>6560
                     /locus_tag="VFX11_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX4940588.1"
                     /translation="MIASSSLLLSGCGGGSADSAKAQSTSTESGNSGTDTGTEQPNTT
                     PHSLSLSSQPGNATLYEGQSTTFTLGVTHNYPVTISWYKNGAKISG"
BASE COUNT         1363 a         2232 c         1912 g         1053 t
ORIGIN      
        1 gataaaaact tcctgggcga agatgttcag gctgttaccc accgagcgcc agtaacccac
       61 gggtaccggc aggtcgacgg gaacatgctc gacccgccgg taaggcaggc ggtaatggca
      121 ctgccgcacc ccctccaccg ccgactcgcc cacccgcttg gacggcacca caccctggcc
      181 gtactccaca gctttaccag gcgccggggc catcaggttt ttcagatact gggtgaggtc
      241 gcctttggcg ggcggtaccg acgccgtttc attcagccgg gcggtactga cgacctgatg
      301 ctgcagcccc gccagctgcc ctttggggcc ggcatggccg cgcatccgga tcactgctgg
      361 tggacggtac tggcctgcag tcagctggtc ctcgcgggtc cacaccacct tgaccggctt
      421 gcgcaggcgc acccccagtt ccagggcttc ggcgataaaa tccgtgccga gccggcggcc
      481 gaacgatccg ccggacagct cgtcgtccag ctggatggcc ttcatcggca agccggtgat
      541 gccggcggcc agcatgcgca cggcatcggg acgctgggtg ccagcctgga tatagcagcg
      601 ctttgatccc acagacacgg tgcaggccag cggctccatg ggggtgtgcg ccagaaatgg
      661 cagacggtaa acgcgctcaa gggcaggcgc cgtggcgggc gccgcgccct cggtgaccac
      721 cacctggctg agtccgggct tgtccagcag gccggccagc gtatcgccca ggtcggcgct
      781 ggacagttcg gcaccacctt tccactgtgc tgccagggcc ttgcgaccct gcagtgctgc
      841 ccaggtgtgt ttcgccacga ccgcaacacc agtctgcagt ggaatcacgg ccgtcacgcc
      901 cggtacggcc agcacggcct gttcattcat ccccgccagc tcggcaccga aacggtccgg
      961 ccgttcaatc gacgccacct gttcgcaggc gcggtcgaaa ccgtatttcg ccacgccggt
     1021 gaccttttcc ttcagccagg cgggttcttt ccattcaccg atatagcgcc agggcggcag
     1081 tgtgggcctg acccgcttcg gatcgaccgc ccgggctgcc tcggtcacct tgccgagcac
     1141 cgcggcgaag cgcagcggct cccgcggacc cagaatccag ccgtcccgca gttcaaatcg
     1201 gggcaacggc cggcccggcg cctgtgcctg cacggtgagt gcggcggcct tcagcagcac
     1261 gtcgcggcag taggcggcgg cctggcgcac gggaacccag ctggtgcggg tggaagcgct
     1321 gcccccggtg ttcagcggct gggccagagc attggcctgg gtcgggccgg aactgagcag
     1381 gcgcacccgg atgcgctcgg gcgccgtacc cagttcttcc gcgaacaggg tgcgcaggcc
     1441 atcgctcagg ccctgcccca tttcatgccg gttcagcaga atgtacagct ggtggtccgt
     1501 accaatttca aaaaagcccg tgatcggtgg cggtgcagtg gaactggccg cgtgggccgt
     1561 cagcggaaac tccagcaaca gggcaccgcc agccgtcagc gtcaggctga tgaaactgcg
     1621 ccggtccagc cggatggaat cagtcatggg aacaactcct ggggcaacgc tgtggggcgg
     1681 catgttatca gttttcgcgc cgttaccgca ggtcaataag aatgggcgtt ttggtcagtg
     1741 tcagcaccgg cgctgaccgc taaggtaggc cgcaccagtc accgcaccca tgaggatgca
     1801 gccatgaaca gcgccgtacc gtttaaggac atgatgcaag cgggtgccag caccactgaa
     1861 caggcgctgg cctggttcga ctcactggac ccggtagacg aggcgttcat gctgggacgc
     1921 tggcgcggtc gcggtgtcgt caccggccat cccatggacg gcctgctgga actgttcaac
     1981 tggtacggga aagagtttct cagcgccgaa gcggtgcatc cgctgctctt cactgccagc
     2041 gacggttccc tggtcaaact ggacccgcgc tggatgtcga tgaagcatgc ccgcaacacc
     2101 ccgctggccc gccacgccat cagccgccgg ctgttcagcg cgctgtcacg actgatgcgg
     2161 accgaacagc ccagggcgcg gctacgcatg atcgagtatc ggggcaaggt gagcgccgcc
     2221 atgcagtacg acaacctgcc gatcaacgat atcttccacc ggatcgatga caatacggtg
     2281 ctgggcgtga tggatctgaa gggaatgcgg caaccgttct ttttcctgct ggaaagggaa
     2341 ggctgacgac ggcagccctc agtgcggggt ggctgccgaa tcggctactc cggcagccgg
     2401 caggtccact gctgcctgca gcagagtacc attgcgggaa tcactgacag gcgcccgact
     2461 cgccagccag tcgtgaatga acggctccag cgccggcccg agggttttcg cgtaactggc
     2521 agacagatgg ttgctgtcgc ggtagaccat tacgttgcca atcaccggtg gacagcgctc
     2581 cgcatcgcac aggtaaccgg tgaggtcgat ctggcgcaac cgttgcggca gaccggcgag
     2641 gtccgcggcg gggttttccg gcagcagcga ctgctgtctt ggcaactgac agcgccggtt
     2701 tgccggcccg aacacgtcga cgcacagggc tggatcggca gcatgccagg gcgtatcccg
     2761 gatcgcgatc acatcgattc ccagcgcctg catctgccac cacttttcca catacttcga
     2821 tggcaccgtt tccgtaccgg ccgcccgcgt cagcgtggtg aacaccgcat cgggccgggc
     2881 gcgctccagt tccttcagca ggttccggtt ccagcggcgg caggactcgc cagcaccctc
     2941 gggaacctga tcggatttgg cctcgtcgct gaaccagcaa ccacttttcg tcatgctcag
     3001 aatccgcagg tcctggcgtt cggcaacggc ctgcaaggct ggcagccact gggcggaatg
     3061 agatcccccc accagcgcaa tcacgtaacg gccatcccgc ttgccgtact cccctacccg
     3121 cacctggtcg tccagcagct gctggtggct ggcatcgctg taggacgccg gcagatcaga
     3181 cttgatgcgg aaggggcccg gcaatggtgg cagcggcacc gcaccgtccg ccttgaatcc
     3241 gggcatcaac gcccgggcac cgggataatc cgcgctgcct acgcggatgc ccgcatcggc
     3301 ttccagcgcc tgcagcctga gggtgtatcc cgcccatacc gcgagaacca gcgcagttgt
     3361 ggtcacacaa ccggcgccaa atgcaaaggc ccgcaccgga cccggacctg ggaaccagtc
     3421 acgccgcacc ggtcgctcga gaaagcgggt ggtggaccag gccagaagca gggataccgc
     3481 caggatcgca aggccctgta ccagactgac ttcgcgcgta ccggaatagg catacaccag
     3541 aatgagcaat ggccagtgcc acaggtagag cgcataggaa atgctgccca gaaactgcag
     3601 cggacgggta cgcagcagag tgccaacacc ccagacgctg ttgttgccgg aacctgccag
     3661 caggatcagc aatgcgccca gcgtaggcca tagcgctgca taccccggaa acaccgtaga
     3721 cacctgaaac aacaggccac agctgaccac tgccagcaaa ccggcccatc ccgccaccac
     3781 cgccgcgacg ccttgcgggc gccacgccat ccggggtacc gccagcagcg atcccagggc
     3841 aaattcccac aatcgtgcgc cagtatcgaa ataggcgaac gcctggttca tgcgggtcgc
     3901 atacacggaa taggcaagag aaagaccaaa cacccccgcc agcacggtca tcagggttgc
     3961 acggacgtcc cgccgcaggc gcgctgacag ccacaccacc atcgccacca gacaaggcca
     4021 aagcaggtaa atctgtccct gggtggacaa cgcccaaaaa tgctggaagg gactgggcgg
     4081 cgtctcccgc gccagatagt caacggcgtc aaaagccaac agccagttct ccaggtaaat
     4141 cgcggcagcg gcggcctcct tgatggcgcc cttccattca accttgggga gaagcaggta
     4201 agaagccaga ctggtaaaca ggataacggt gatcgcggca ggcaaaagcc gcttcagcag
     4261 acgggcccag aagccagcga aatcaacccg gccctgccga tccacctggc gcagcagcat
     4321 acccgtaatc aggaagccgg agaccacgaa aaacacgtcc acaccgccgg acacgcggcc
     4381 aaaccacagg tggtaagcac acaccagcag cgccgcgacg gcacgcaaac cctgaatatc
     4441 gggcctgaat ccctgcttga gaactgaacc gttcaatctc accccaatac gaatgcgcaa
     4501 taggtgcacg ccatatttcc ggtcattgtg ccccggccgg tggccgcgcg gtgcgctaaa
     4561 aatcaatgca atggcaggtt tttcttaccg ttcccggact gcaatccctg gagcaaacgg
     4621 tgtgtgaacc tgagcccggg atactaccac gacgcaggca aacgctaagc atgccagtcg
     4681 ggcagaaaga cctgactcta cagcctgccg actgccggtc gccacgacgg acaaacggcc
     4741 ggcccgacca gcactgtaca aaaattgcgc agacctgtca ctctccccac ccggcaccct
     4801 cacgcattct cgttcccgca gtgaactccg tcccagcttg gccgctcatt tcttccgcat
     4861 ttacgaactg agaactgacc cgcaaggaca cccccacccg gtttctatat tggttgccag
     4921 gaattcgacc tacccggccc ctgttcaaac tgaaggtgta atcacgatgt cgcttgtctc
     4981 tctctgcaaa accctgttca aagtgcacgg aatggcgctg gttctgacca cttccggcct
     5041 tctgctgggc ggctgcggag gcggctccca gagcagcggc agcaccgaac cccctgtcca
     5101 gaccgatggc ggcagtacag acggctccgg cacgcagacc ggcggcaccg atcccacgcc
     5161 cacgcccacg ccagcaccgg aacaacacag tctcagcctg acttcgcaac cagccaatgc
     5221 cgtcatttat gaaggccaga accagacatt cagtctgacc tacagcaata gccacccggt
     5281 aagcgtggcc tggtactaca acggcagccc gatcagcggc gccagcggca gcagctacac
     5341 agtgagcaac gccaccaccg cccgcgccgg cacctattcc tgcgccgtga ccgatggcac
     5401 cctgaccgtc acctgtggca gcttcagcct cacagtccac cagatcgtcc gcatcaccca
     5461 acagcccatc aaccagatag tgaatgaagg tgtaaacgcc aacttcagtg tcaccgctac
     5521 cggcacgggc cccgtgagct accagtggta cttcaacgac cagccgctga ccggcgccac
     5581 cagcgcccag ctgaacctgg ctgccacctc cagcgccaac agcggccagt accacgtgcg
     5641 ggtttccaac ggcggctcca gcgccaacag cagcaaggcc acgctgaccg tcatcagcaa
     5701 cccgaatggc aaggtcgtct tgagctggga cgcgcccacc aggcgtgcgg atggttctaa
     5761 cctggctgcc ggcgatatcg ccagctacca gatcttccac gcggattccg ccacgggcgc
     5821 gctgacccag ctgaccagcg taaccggcgc ggaactgagc ctgcaggtgg aggagctggc
     5881 catcggcacc cactacttcg ccctgaccac cgtggacacc cagggactgg tcagcgctca
     5941 atccagcagg atcgccgtcg cagtccagta accggtaaag ggaggaggcc gcgcttcctc
     6001 ccgtttacta cccgcgctaa acccatcaca tcgggcaaga cacaggccac agtccggact
     6061 gaaccccgct cactgcccgc ctgtgacttt ctgctcaatt tttatatgaa ttttgatcga
     6121 tccatcaagc tgtgaactgg cccacacgcg tgccggagcc tgccgactac tctgaccctt
     6181 atagggttcg tgcgaaccgc tcccatggac acaaattcga aaggtattca acgtgatggc
     6241 catttcatcg atagcacaag gacttttcag ggcacctgcg attgctctca tgatcgccag
     6301 ctcgagcctg ctgctgagcg gttgtggtgg aggcagcgcc gattctgcca aagcccagag
     6361 cacatccact gaatccggaa acagcgggac cgacaccggc accgagcagc cgaacacaac
     6421 accccactcc ctgagcctca gttcccagcc tggcaacgcc accctctatg aagggcagag
     6481 cacaaccttt accctgggcg taacacacaa ctatcccgtc accatcagct ggtacaaaaa
     6541 cggcgccaag atcagcggcg
//
LOCUS       DASUEG010000961         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1416118, whole genome shotgun sequence.
ACCESSION   DASUEG010000961 DASUEG010000000
VERSION     DASUEG010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416118"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFX11_18090"
     CDS             complement(<1..667)
                     /locus_tag="VFX11_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4940589.1"
                     /translation="MNTVITSPLVQSARNWLAREAPALPADLQAAIESLYEAVEAGHV
                     CIRVDARNATSWRATGWVGAPGDYCPFILEDSGRFYLARYHDHEEGVAASLKARAEKL
                     LTPNDAVALTHELHTLFPEDDPDDRQRLAALLAQFKALTLVSGGPGTGKTTTVVKLLA
                     LLQSQAGDTPLHILLAAPTGKAAQRLSESIRGAKEKLPVTDDVKAVIPEQAQTLHRLL
                     GAQG"
     gene            complement(664..>2278)
                     /locus_tag="VFX11_18095"
     CDS             complement(664..>2278)
                     /locus_tag="VFX11_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4940590.1"
                     /translation="AAFNVVERVLSGAHGERRLTNLRHLATLLQQQADTDPVPERQLA
                     WLREQVREAETTEDTQLRLESDAERVQVLTIHVSKGLEYPVVFCPFLWDGSLMRRDDV
                     HRAEYRAGGHSLLDIGSDKFDHATTRMATERMAERLRVLYVALTRAKYRCYGVWGWTK
                     DSETAPFSWLLLGGNTEGDPDADVLSRDLERFDHDDYAAALDRVAAKVPDGFAWQSLS
                     DDIAMTTMPVQTAPDGEPVVPAFTRTLWRRWRVSSFTGLSESAHDVSRKGESPDHDTD
                     VQALVTPDVVLQEAPQEPQGIHAFPRGANAGVFWHAVLEDCVGSPSVVRSAVITERLQ
                     QHALPLDLQPLVDSTLAQLLETPLDEEGARLGHLEAARAEMEFLYPVQQLSPAQFLSL
                     PDVLPRYRDALTALDFPTLQGYLKGFIDLVYRHQGRYYVLDYKTNWLGPDEGAYTQAR
                     MEEAMAHSHYYLQYWLYVLALHRHLKAVKPDYDYERDIGGVRYLFLRGISATTRGIYA
                     RKPSRALIEALDALMSGRAVRGTSAGVPT"
BASE COUNT          450 a          679 c          721 g          428 t
ORIGIN      
        1 caccttgcgc gccaagcaag cggtgcagcg tctgggcctg ttcgggaatg accgctttca
       61 cgtcgtcggt gacggggagc ttttccttgg cgccgcgaat ggattcggag aggcgttgcg
      121 cggcctttcc ggtaggggcc gcaagcagga tgtgcagcgg tgtatctccg gcctgcgact
      181 gcaacaaggc cagcagtttg accacggtgg tggtcttgcc ggtgccgggg ccaccactca
      241 ccagcgtgag cgctttgaat tgggcgagca aggcggcgag gcgctggcgg tcgtctggat
      301 catcttccgg gaagagcgtg tgcaactcgt gcgtgagcgc tacggcatcg ttgggggtca
      361 gcagcttttc agcacgcgct ttcaatgatg ccgccacgcc ctcttcatga tcgtgataac
      421 gggcgagata gaagcgaccg ctgtcctcca gaatgaaggg acagtaatcg ccgggagcgc
      481 ccacccaacc ggtggcgcgc catgaagtgg cgttacgggc atcaacgcgg atgcagacat
      541 gaccggcttc gaccgcttcg tacagtgatt cgatggcggc ctgcaggtct gccggcaggg
      601 ccggtgcttc gcgtgccaac cagttgcggg cggattgaac cagcggcgac gtgatcacgg
      661 tgttcatgtt ggtactcctg ctgacgtgcc acgcactgcc ctgccgctca taagcgcatc
      721 gagcgcctcg atgagtgcgc gcgacggctt acgtgcgtaa atgccgcgcg tggtcgcgct
      781 gataccccgc aggaacaggt agcgcacgcc accgatatca cgctcgtagt cgtaatcggg
      841 tttcacggct ttcaggtgcc gatgcagggc cagcacgtac agccagtact gcaagtaata
      901 atgtgaatgg gccatggctt cttccattcg cgcctgcgtg taggcacctt cgtcaggacc
      961 caaccagttg gtcttgtagt cgagcacgta gtaacgaccc tgatgacggt agacaaggtc
     1021 gataaagcct ttgagataac cctgcagcgt agggaagtcg agcgccgtaa gggcgtcgcg
     1081 gtaacgcggc agcacatccg gcaacgacag gaattgtgca ggtgaaagct gctgaaccgg
     1141 atacaggaac tccatttcgg cacgagcagc ctccagatgc ccgagtcggg caccctcctc
     1201 gtccagcggc gtctccagca actgtgccag cgtgctgtct accagaggtt gcaagtccag
     1261 cggcagtgcg tgctgctgca ggcgttcggt aatcacggcc gagcgaacta ctgaggggct
     1321 tcccacacaa tcttcgagaa cggcatgcca aaaaacacct gcattggcgc cgcgcgggaa
     1381 ggcgtgaata ccttgtggtt cctgtggagc ctcttgcagg acaacatcag gtgtgactag
     1441 tgcctgcacg tcggtatcgt ggtcggggct ttcacccttg cgtgacacgt cgtgggcgct
     1501 ctctgaaaga ccggtaaaac tgctcacgcg ccagcgacgc cacaacgtgc gcgtaaaagc
     1561 aggaacgaca ggctcaccat caggggcagt ctgtaccggc atggtcgtca tggcgatgtc
     1621 atccgacaag gactgccagg caaaaccgtc ggggactttc gcagccacac ggtccagcgc
     1681 agcggcgtaa tcatcgtggt caaaacgttc cagatcacgc gacagcacgt cagcatcggg
     1741 atcaccttca gtattaccgc ccagcagcag ccagctgaag ggcgcggttt cgctgtcctt
     1801 ggtccagccc cacacgccat agcaacgata cttggcgcgc gtcagggcaa cgtagagcac
     1861 acgcaaccgt tcggccatac gttccgtggc catgcgggtg gtggcatggt cgaatttgtc
     1921 cgagccgata tccagcagcg agtggccacc ggcgcggtac tcggcgcggt gtacatcgtc
     1981 acggcgcatc agcgacccgt cccacaggaa aggacagaac acgaccggat attcgaggcc
     2041 cttgctaacg tgaatggtaa gcacctgcac acgctcggca tcgctttcca gtcgcagctg
     2101 ggtgtcttca gtggtttccg cttcacgcac ctgttcgcgc agccaggcca gttggcgctc
     2161 cggcaccgga tcggtatcgg cctgctgctg aaggagcgtg gccaggtggc gcaggttggt
     2221 gaggcgacgc tcgccgtgcg caccacttag tacgcgctcg accacgttga aggcagcc
//
LOCUS       DASUEG010000962         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_787932, whole genome shotgun sequence.
ACCESSION   DASUEG010000962 DASUEG010000000
VERSION     DASUEG010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787932"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFX11_18100"
     CDS             complement(<1..964)
                     /locus_tag="VFX11_18100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX4940591.1"
                     /translation="MDGDPAKILLVDDVAENLVALEALLQDPAHETFSATNANDALEL
                     MLQHEFALAVIDVQMPDMNGFELAQIMRSTERTRQIPIIFVTAGSTKPDNVLMGYQAG
                     AIDFMIKPLDNYAVLSKVRIFVELYQQKMALNRQVAELEEARSQQHQLLEKLQIAQVE
                     LEKAMELRDQFISVVAHELRTPLNTLNLELYARRTYLERGDYSQFTPEHIAEMVEGDE
                     RQLKRLIRLINDMTDLSRIQSGQLSMQCKRFDVTALLRRVVALFSAQIALARCSVTFH
                     GEPGVIINGDEFRIEQAFTNLLTNAIRHCNEKPIEIRVQELAGMV"
     gene            complement(1076..1942)
                     /locus_tag="VFX11_18105"
     CDS             complement(1076..1942)
                     /locus_tag="VFX11_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX4940592.1"
                     /translation="MNRAPDDNQLIRLWNAQEEAEFSALLDDIYHLHNHDFRGYSGAF
                     LRRRLSLVMSRLDLGSVDQLRIRINSDPRSFLTVFQHLTVSVSEMFRDPLYFRCLRDD
                     VFPILKTYSSPRIWVAGCGAGEEVYSIAILLHELGMLECSLIYATDINPIALKTASLG
                     IFPVANLPAYTLNYRAAGGESVLADYYHVAYGTAVFDRQLRRRIVFAEHSLATDSIFS
                     EVQFVSCRNVLIYFSREMQGRTVNMFADTLCHRGFLGLGAREGIAVSGAAARFDNFAK
                     PVKIYRKRAEFY"
     gene            complement(1939..>2561)
                     /locus_tag="VFX11_18110"
     CDS             complement(1939..>2561)
                     /locus_tag="VFX11_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017133867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940593.1"
                     /translation="SAADLGSFPPVVIYAQRSLKPEEEASLLRYSHSIVVKGARSPER
                     LLDEVMLFLHKIETNMSSEHRQLLQSARSPRRALEDRKILLVDDDVRNVFALTSALES
                     RSALVEIARNGIEAVEKVKTVDNIDLVLMDMMMPEMDGYQAMIEIRKDPRFRKLPIIA
                     VTAKAMKDDQERCLRAGANDYVAKPVDIDRLLSLIQVWLPKTKLSP"
BASE COUNT          625 a          735 c          602 g          599 t
ORIGIN      
        1 tcaccatgcc cgcgagttcc tgaacccgaa tttcaatggg cttttcattg caatgacgaa
       61 tggcattcgt caacagattg gtaaaggcct gttcgatgcg aaattcatcg ccattgataa
      121 taacgcccgg ttctccgtga aaagtgaccg agcatcgggc caacgcaatc tgcgcagaaa
      181 aaagagcaac cacccgacgc agcagagcag tcacatcaaa gcgtttgcac tgcatcgaca
      241 actgtccgct ctggatcctg gacagatcgg tcatatcgtt gataagccgg atcaggcgct
      301 tcagttgacg ctcatcgcct tctaccattt cggcaatatg ctcaggcgta aactgcgaat
      361 aatctccccg ctccagatag gtacggcgcg catacagttc cagattgagt gtattcagtg
      421 gcgttcgcaa ttcatgcgcc accaccgaaa tgaactgatc cctcagctcc atcgctttct
      481 cgagctccac ctgcgctatc tgcaatttct ccagcaactg atgttgctgg gatcgcgcct
      541 cctccagctc ggcgacctgt cgattcagcg ccatcttctg ctggtacagc tcgacgaata
      601 ttctcacctt gctgagcacc gcatagttat ccaggggctt gatcatgaaa tcaatggcac
      661 ccgcctggta gcccatcagc acgttgtctg gtttggtact gcctgccgtt acgaatatga
      721 tgggtatctg ccgcgtgcgc tcggtagagc gcatgatctg cgccagttca aatccattca
      781 tatccggcat ttgcacatca atcactgcca gggcaaactc gtgctgcaac atcagctcga
      841 gcgcatcatt ggcgttggta gcactaaagg tctcgtgcgc tggatcctgc aacagcgctt
      901 ccagcgccac caggttttca gcaacatcat ctaccagcag aattttcgcc ggatcaccat
      961 ccattctttc cctcctgaat ctccctgatg ccacccgcat tttcaacggt tgaataagca
     1021 ggcaacgcaa ccacacccga cttccatcta tccctggtgt tcagcgctcg ttcattcaat
     1081 agaattccgc ccttttcctg tatattttga ccggtttcgc aaaattatcg aaccttgccg
     1141 ctgcaccgga cacggcaatg ccttcccggg cgcctagccc aagaaatccc cggtgacaca
     1201 acgtatcagc aaacatgttg acagtgcgcc cctgcatttc ccgactgaaa tagatcaaaa
     1261 cgtttcggca cgacacaaac tgcacttccg aaaatatcga atcggttgcc aggctgtgct
     1321 ctgcgaacac aattctgcgc cgaagctgac gatcaaaaac cgcggttcca tatgcgacat
     1381 gataatagtc tgccaggaca gattcaccac cagcagcacg atagttcaag gtgtacgcag
     1441 gaagattggc aactggaaaa atgcccagcg atgcagtttt caacgcgatt ggattgatgt
     1501 cggtggcata gatgagactg cattccagca tgcccaattc atgcaaaagt atcgcgatgg
     1561 aatagacctc ttctccggca ccacatccgg ccacccagat cctcggcgaa gagtaggtct
     1621 tgaggatcgg aaacacgtcg tcacgcaaac agcggaaata cagtggatcc cggaacatct
     1681 cgctaacgga cacagtcaga tgctggaaaa ccgttaaaaa actcctggga tcactgttga
     1741 tgcggatgcg tagctggtcc acgctgccaa gatcgagcct gctcatgacc agactcagcc
     1801 ggcggcgcag aaaagcaccc gaatagccac gaaaatcgtg attgtgcagg tggtaaatat
     1861 catccagcaa tgcggaaaac tcagcttctt cctgagcgtt ccacaatcgg atcaactgat
     1921 tgtcgtcagg tgcacggttc atggggacaa ttttgttttt ggcaaccaga cctgaatcag
     1981 cgacagaagg cggtcaatgt caaccggctt cgccacataa tcatttgcgc cggcgcgcaa
     2041 acagcgttcc tgatcatcct tcatggcttt tgccgttacc gcaatgatgg gaagcttcct
     2101 gaaacgggga tccttgcgga tttcgatcat ggcctgatat ccatccattt caggcatcat
     2161 catgtccatc aagaccaggt ctatattgtc gactgtcttc actttttcca ctgcctctat
     2221 accattgcgg gcaatttcaa ccaacgcgct tcggctctcc agtgcgctgg tcagtgcgaa
     2281 tacattgcgg acgtcatcat caaccagcag gatttttcta tcctccagtg cccgcctggg
     2341 tgagcgcgca gactgcagca gttgccggtg ctcgctcgac atattggttt caattttatg
     2401 caggaacagc atgacttcgt ccagcagccg ctccggcgat cgggcaccct tgacgactat
     2461 ggaatgggaa tagcgcaaca ggctggcttc ctcttccggc ttgagactgc gttgagcata
     2521 aatcactaca ggtggaaagg aaccgaggtc tgctgctgac a
//
LOCUS       DASUEG010000963         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1714901, whole genome shotgun sequence.
ACCESSION   DASUEG010000963 DASUEG010000000
VERSION     DASUEG010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714901"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2773
                     /locus_tag="VFX11_18115"
     CDS             <1..>2773
                     /locus_tag="VFX11_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4940594.1"
                     /translation="RTVGWFTSIYPVVLQLDAQADLGAHIRAVKEQLRAIPRKGMSFG
                     VLRFLSEQPERRQLLNSLAQSQISFNYLGQLDQVLADDALLQQATESTGDELAPAGQR
                     KYLLDISGRVQNGELILAFRYSHSVHQSATIEKLASGLVNELTSIIEHCGDLSHAAYT
                     PSDFPLARLDQQQLDSLLPRRGVETVYPLSPVQEGMLFHSLYEPGSWVYFDQLIVPIN
                     GAVDETALFDAWRLVVARHQILRTGFIWDGVQQPLQIVMEQIDLDIHRYDWRKLDAAS
                     AQEKFQRYLIDDRNLGFNFKRPGVLRLSWIAMPGDEHRLLWSFHHILLDGWSMPVVFG
                     ELFTAYEALAQGRTPEWPAAPRYQDFIGWMLVQDRAKAQAFWRKYLKGFTAATPIPVK
                     NSAAVVAHRARSGDAASRYREQEIRISGVLLEGLQQLAKQHRLTLNHVMQGIWAVLLQ
                     RYSGENDVLFGTTVSGRPAELPGIENMVGLFINTLPLRMQVDRHAPFIDWLQSVQETQ
                     AEMKHYEYSSLVDVQKASELPPKQPMFESILVFENYPIDETLEQTQQSLQIGNPDAFQ
                     QTNFPLTLVAIPGRDLLVRFSYDSHLFDADTIERMLAHIETLLSAVHANPASVIEHLP
                     LITEREYEHLVYELNDTIRPFPSESTLAELFEQKAAQYARQIVCEDGDQLITYERLNS
                     KANQLARLLISNGVKPADPVLLCFDRSLSMVECMLAVLKAGGAYVPVDPAYPEDRIRH
                     MAEDTRPPVVVTLKQHSSRFDFLQGTGTKVLELETLAPALQGQPDANLNIRPQQGSHA
                     LACVIFTSGSTGRPKGVCIPQIGVARMVQNPDYMVMAPGHRLGHASAIAFDAATFEIW
                     TPLLNGGTLVVIERDIALDPERFARFIKQKKITIQFLTTALYNVISKQVPDVFSDVDY
                     CLFGGEACDP"
BASE COUNT          547 a          800 c          844 g          582 t
ORIGIN      
        1 cgcgcacggt aggctggttc acctcgattt atccggtggt gctgcagctg gacgcacagg
       61 cggatctggg tgcgcatatc cgcgcggtga aagaacagct gcgggccatt ccgcgcaagg
      121 gcatgagctt tggcgtgctg cggttcctgt cggaacagcc ggagcggcgc cagttgctga
      181 attcactggc acagagccag atcagcttca actatctggg tcagctggat caggtgctgg
      241 cggacgacgc gttgctgcag caggcaaccg aaagtaccgg cgatgaactg gcgcccgccg
      301 gtcagcgtaa atacttgctg gatatcagcg gccgcgtgca gaacggtgaa ctgatcctgg
      361 cattccgtta cagccattcg gttcatcagt ctgccactat cgagaagctg gccagtggtc
      421 tggtgaacga actgaccagc atcatcgagc actgtggtga tctgtcccat gccgcttaca
      481 caccgtcaga ttttccgctg gcgcgactgg atcagcagca actggattct ctgttgcccc
      541 gccgtggcgt ggagactgtt tatccattgt cgccggtaca ggaaggcatg ctgttccaca
      601 gtctgtatga accgggttcc tgggtttact tcgatcagct gatcgttccc atcaacggcg
      661 ccgtcgatga aacggcactg ttcgatgcct ggcgtttagt cgtggcgcga caccagatcc
      721 tgcgcaccgg ctttatctgg gatggcgtgc agcaaccgct gcagatcgtg atggaacaga
      781 tcgatctgga tattcaccgc tacgactggc gcaagctgga cgcggcgtcg gcgcaggaaa
      841 aattccagcg ttatctgatc gatgaccgta acctgggctt caatttcaag cgccccggtg
      901 tgctgcggct gagctggatc gccatgccgg gcgatgaaca ccgcctgctg tggagcttcc
      961 accacattct gctggatggc tggtcgatgc cggtggtgtt tggtgagctg ttcacggctt
     1021 atgaagcgct ggctcaaggt cgcacaccgg aatggccggc ggcgccacgc taccaggatt
     1081 ttatcggctg gatgctggtg caggatcgtg cgaaggcgca ggccttctgg cgcaaatacc
     1141 tgaaaggatt cactgccgcc acgccgattc cggtgaagaa ttcggctgct gtcgttgcac
     1201 accgtgcccg cagtggtgac gccgcgtccc gttaccggga acaggaaatc cgtatatccg
     1261 gcgtcttgct ggaaggactg cagcagctgg ccaaacagca ccgcctgacc ctgaatcatg
     1321 tcatgcaggg tatctgggcc gtgttgctgc agcgttacag tggcgagaac gacgtgctgt
     1381 tcggtaccac cgtatcgggt cgccccgcgg agctgcccgg catcgagaac atggtgggcc
     1441 tgttcatcaa tacgctgccg ctgcgcatgc aggtggatcg tcatgcgccc tttatcgact
     1501 ggttgcagtc ggtgcaggaa acccaggcgg aaatgaaaca ctacgaatac agctctctgg
     1561 tggatgtgca gaaagccagt gaactgccgc ccaagcagcc gatgttcgaa tccattctgg
     1621 tgttcgagaa ctatcccatc gacgaaaccc tggagcagac gcagcagtcg ttgcagattg
     1681 gcaatcccga cgcattccag cagaccaatt tcccattgac gctggtggcg attcccggcc
     1741 gcgatctgct ggtgcggttc tcctacgaca gccacctgtt cgatgcggat accatcgagc
     1801 gcatgctggc ccatatcgaa acgctgctct cggccgttca cgccaatcca gccagtgtga
     1861 ttgagcattt gccgctgatc accgagcggg agtacgagca tctggtgtat gaactgaatg
     1921 acaccattcg cccgtttccg tccgaatcca cgctggcgga actgttcgag cagaaggcgg
     1981 cgcaatatgc ccgccagatc gtgtgcgaag acggcgacca gctgatcaca tacgagcgcc
     2041 tgaacagcaa ggcgaaccag ctggcgcgtc tgctgatttc caatggcgtc aagcctgctg
     2101 atccagtgct gctgtgcttt gaccgttcgc tgtcaatggt ggagtgcatg ctggcggtgc
     2161 tgaaggccgg cggcgcttat gtaccggtcg atcccgccta tcccgaggat cgcatccgcc
     2221 atatggccga ggacacgcgt ccaccggtgg tggtgacgct gaaacagcac agcagccggt
     2281 ttgatttttt gcagggcact ggcaccaaag tgctggagct ggaaacgctg gcgccggcat
     2341 tgcagggtca gcctgatgcg aacctgaaca tccggccgca gcagggcagc cacgcactgg
     2401 cctgcgtgat tttcacctcc ggttccaccg gccggccgaa aggtgtgtgc attccgcaga
     2461 tcggcgtggc gcgcatggtg cagaatccgg attacatggt aatggcgccg ggtcatcgtc
     2521 tgggccatgc ctctgccatc gcgttcgacg cggccacttt cgaaatctgg acgccgctgc
     2581 tgaacggcgg cacgctggtg gtgatcgaac gggatatcgc actcgatccc gaacggtttg
     2641 cgcgtttcat caagcagaaa aaaatcacca tccagttcct gaccacggcc ctgtacaacg
     2701 tgatttcaaa acaggtaccc gatgtgttca gcgacgtgga ttactgcctg tttggcggcg
     2761 aggcctgcga tcc
//
LOCUS       DASUEG010000964         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1017158, whole genome shotgun sequence.
ACCESSION   DASUEG010000964 DASUEG010000000
VERSION     DASUEG010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017158"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFX11_18120"
     CDS             <1..722
                     /locus_tag="VFX11_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4940595.1"
                     /translation="LAGILVVGPRLGRFDPVTGQARTIPGHNLGMVALGGFILWLGWF
                     GFNAGSTLQANMSLGKIALNTHLAACAGAAGTTLLLALTGKPILLTLTVNGSIAGLVA
                     ITAGCATMEPGYAILTGLVAGIIVVAGTRALELLLLDDAVGAIPVHGLAGVWGTLAAG
                     LFFMDDPFNAQRIWVQAVGCIVAFLWSFPLAFTMYWLLEKTVGLRASSLQQQRGLDYA
                     EHHETGYPEFDQLHLHKPLTP"
     gene            745..1398
                     /locus_tag="VFX11_18125"
     CDS             745..1398
                     /locus_tag="VFX11_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940596.1"
                     /translation="MEERIRQRRALYTVLRQWLDENNLLEALQVFEDQFQGQPTIAVN
                     DYLNRIAPLYRDRVDAKTLRHNLMQLLVRKSAQLEPDPLPLLQQLRARTESVALEKKG
                     AQATAEQLALHTLVSNMMRKLAQHQRKQVHAAIALQLKQHFVNGSYDALARYLLTDNP
                     LHLALFDDRSLRQFFNLIYVAACEVLGPTSADRWFGSCIAQLRETDRVAGDAVNRYL"
     gene            complement(1408..2223)
                     /locus_tag="VFX11_18130"
     CDS             complement(1408..2223)
                     /locus_tag="VFX11_18130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4940597.1"
                     /translation="MPFSDDAYSLLYLWSQRTIYIGAMFELSCFTPAASALVISMEKP
                     LRIRSAASPDFIATRSALIPAGVSVEVEDTDQLMACCYLDALGRDLAAFKTLMQRQVG
                     NLWIDSRIERQQIASYELIYRTRMPAAQTYKTLLELIFPPAAVCNAVPGARAEIARVV
                     EIIRCDPVSNPSNLQLAQQVGLTEAQLQRQFKQITGIPIRRYRLWHRLFVTATLMMLG
                     KTLTDAALEAGFSDSSHFNHAFRSILGMKPSFIFQRRDRIFIYAGGDVEAGDG"
     gene            2420..3379
                     /locus_tag="VFX11_18135"
     CDS             2420..3379
                     /locus_tag="VFX11_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX4940598.1"
                     /translation="MSLHRVVAGTFLCLLFSLSTSNALAISADYAKTRYPIVMVGGFL
                     AFDSIFGIEYFYGVADNLRRYGATVYESDVSEFQTNETRGEELLAQIEEYLAVTGHKK
                     VNLVAHSQGSPTARYVAAMRPDLVASVTSVHGMNRGTQIADFFKQVAPEGSTTLALLE
                     ALSQAMGFLWTTLSGGPNPTGQSAAAIIQGADPVDYERFNKRYPQGMPTSACGTSGAA
                     QVNGVRYWSWGGTGGLFFYQTNPLDLLDTAMYGITPLMFSKGVKHDGVVPLCGQYLGT
                     PIRHNYAQNHTDAQNQLFGLTGVTVNPVTLFRDHANRLKKAGL"
     gene            3376..4476
                     /locus_tag="VFX11_18140"
     CDS             3376..4476
                     /locus_tag="VFX11_18140"
                     /inference="COORDINATES: protein motif:HMM:NF015252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HEX4940599.1"
                     /translation="MIERQTATKPVRLASARSAGNRNRPGVTTGLLLLTLVALGGGWY
                     WQSTKVAGKHGDEKGVPASTASMAQETSVVLLTTPTEAIADDTAPTDVQQALSDTSLA
                     GLEIPNSLMMDGDQLVANEGLRELMDFFLTLTGEREPAQIRALFAAAATAQCAAICAE
                     QALVIYDHYLEYLRAMETASADLQDTDDLRSRLQRVAGIRSEFLGEEVAKSLFAAENA
                     YDNLRVAQWEIRTQTDLSAAEKSEALAALESSLPPDLAAREQERKLLRSVRNLNRTLD
                     EQQKDAADRYAARAALLDVAAAERLQALDTRRAEWAQRYEAYRQERTRLDISGLDTVD
                     RQQALEQLRLRHFSAQESQRAAALDRVNGLER"
     gene            complement(4552..>5314)
                     /locus_tag="VFX11_18145"
     CDS             complement(4552..>5314)
                     /locus_tag="VFX11_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940600.1"
                     /translation="AFDSAIVFYASTQDPRLSSFTGSPRAQFIYFDADEIGWGDESER
                     FPFNTRDDFHHYRIEVESDGMARFYADGQLALQRADFKLHPRLAFGDMTNDPAVNGRF
                     SLSGMTVSGRHTTLVSADFRPSTCPNPLNAHGRGRVPVAIAGTTAFNVNRIDPESVRI
                     NGVPAQAWSIRHATRPVLPYVGKVNVSDCTANGSDGRLDLVLAFDEQALLASLGSIYN
                     GQVTALRVTGKLKPEFGVQAFAGEDIVRIINVRPH"
BASE COUNT         1109 a         1590 c         1593 g         1022 t
ORIGIN      
        1 cgctggcggg cattctggtg gtgggtccgc ggctggggcg tttcgatccc gttaccggac
       61 aggcacgcac gattcccggc cacaatctgg gcatggtggc gctgggtggt ttcattctgt
      121 ggctgggctg gttcggcttc aacgccggca gcacactgca ggccaacatg agcctgggca
      181 agattgcact gaacacccat ctggccgcct gcgcgggtgc tgccggtacc acactgctgc
      241 tggcactcac gggcaagccg attctgctga cactcaccgt gaacggcagc attgccggcc
      301 tggtggcgat caccgctggc tgcgccacca tggaaccagg ctacgccatt ctcaccgggc
      361 tggtggccgg catcatcgtg gtggccggaa ccagggcgct ggaactgctg ttgctggacg
      421 acgcggtggg cgcgataccc gtgcacggcc tcgccggtgt ctggggcacg ctggcagcag
      481 gtctgttttt catggacgat ccgttcaacg cacagcggat ctgggtgcag gccgtgggct
      541 gtattgtcgc tttcctgtgg tcatttccgc ttgcgttcac catgtactgg ctgctggaaa
      601 aaaccgtcgg cctgcgcgca tcgagcctgc agcaacaacg gggactggat tatgccgaac
      661 atcatgaaac cggttatccc gagttcgatc agctgcatct gcacaaaccc ctgacacctt
      721 aattgcccgc caacaggtag ccgtatggaa gaacgtattc gccagcgccg cgccctttac
      781 accgtgctgc gacaatggct ggatgagaat aacctgctgg aagcgctgca ggtgtttgaa
      841 gaccagtttc agggccagcc caccatcgcg gtgaatgatt acctcaaccg cattgcgccg
      901 ctgtaccgcg atcgggtgga tgcaaaaacc ctgcgccaca acctgatgca gttgctggtg
      961 cgcaaatcgg cacaactgga gcctgatccg ctgccgctgc tgcaacagct gcgggcgcgc
     1021 accgaatccg tggcactgga aaaaaaaggc gcgcaggcca cagcggaaca gctggcgctg
     1081 cacaccctgg tgtcgaacat gatgcgtaag ctggcgcagc accagcgcaa gcaggtgcat
     1141 gccgccatcg cgttgcaact gaagcagcac ttcgtcaacg gcagttacga cgccctcgcc
     1201 cgctacctgt tgaccgacaa cccactgcat ctggccctgt tcgacgaccg cagcctgcgc
     1261 cagtttttca acctgattta cgtggccgcc tgtgaagtcc tgggcccgac cagtgcggac
     1321 cgctggttcg gcagctgcat tgcgcagttg cgggaaaccg atcgggtcgc tggcgacgcc
     1381 gtgaaccgct acttgtagca gaacctgtca gccgtctccc gcctccacgt cgccaccggc
     1441 gtaaataaaa atgcggtcgc gtcgctggaa tatgaacgac ggcttcattc ccagaatgct
     1501 gcgaaacgca tgattgaagt gggatgaatc ggaaaagcct gcctccagcg ccgcatcggt
     1561 gagagttttt cccaacatca tcaaggtggc cgtcacgaac agccggtgcc acaaccggta
     1621 acgccggatg ggaatgcccg taatctgttt gaactgccgc tgcagctgcg cctcggtcaa
     1681 tccgacctgt tgcgccagct gcaggttcga cggattggac accggatcac accggattat
     1741 ttccaccacc cgagcaatct ccgcacgcgc accaggcact gcgttgcaca cggctgccgg
     1801 tggaaaaatc aattccagca aggttttata ggtctgcgct gccggcatcc gcgtgcgata
     1861 aatcagttca tagctggcga tctgctgccg ctcgattctg ctgtcgatcc agaggttgcc
     1921 tacctgtcgc tgcatcagag ttttgaatgc cgccagatca cggcccagcg cgtccaggta
     1981 gcaacaggcc atcaactgat cggtgtcctc cacctctacc gacacgccag cgggaatcag
     2041 tgcactgcgg gttgcgatga aatcagggct ggcggcactc cggatccgca gtggtttttc
     2101 catgcttatc accagcgcgg aggccgccgg ggtaaaacag gacaattcaa acatggcacc
     2161 gatgtaaatc gtgcgctggc tccacaggta aagcagggaa taggcatcgt cgctgaaagg
     2221 catggtccgg tgtcgttgtt tttattatga atcgggacgg ggagtgctcc gcatgaccgc
     2281 cctgttcaat tcatacaagc gcggccgcgt cggaatattc tattgtgaaa atgagcgaat
     2341 aataaaggtg attcctaaaa acaggctctc ttcataactc aataaaaaac ttaaaacaaa
     2401 aaccaaagga aatttgctta tgtcactcca tcgcgtcgtg gcaggtacct tcttgtgcct
     2461 gctgttcagc ctttccacca gtaacgccct tgccatcagc gccgattacg ccaaaacccg
     2521 ctatccgatt gtcatggtgg gtggttttct ggcgtttgat tccattttcg gtatcgaata
     2581 tttctacggt gttgccgaca acctgcgccg ctacggtgcc actgtgtacg aatcggatgt
     2641 cagcgaattc cagaccaacg aaacccgggg cgaggaattg ctggcccaga tcgaggaata
     2701 tctggcagtg acagggcaca agaaagtgaa cctggtcgct cacagccagg gatcgcccac
     2761 tgcgcgctat gtggcagcca tgcggccgga tctggtggcg tcggtcactt cggtgcacgg
     2821 catgaaccgg ggcacgcaga tcgccgattt cttcaagcag gtggcgccgg aaggcagcac
     2881 cacactggcg ttgctggaag cgctgagcca ggccatgggt tttctctgga ctacactgtc
     2941 tggcggcccc aatcccaccg ggcagtccgc cgccgccatt attcagggcg ccgatccagt
     3001 ggactacgag cgcttcaaca agcggtatcc gcagggtatg cccaccagcg cctgcggcac
     3061 cagcggcgca gcgcaagtca acggcgtgcg gtactggtcc tggggtggca ctggcggcct
     3121 gttcttctac cagaccaatc cgctggatct gctcgacact gccatgtacg gcatcacacc
     3181 gctgatgttc tccaagggtg tgaagcatga cggcgtggtg ccgctgtgcg gccagtatct
     3241 gggtacgcca attcgccaca actacgcgca gaaccatact gatgcacaga atcagctgtt
     3301 tggtctgacc ggagtgacgg tgaatccggt gacgctgttc cgcgatcacg ccaaccggct
     3361 gaaaaaggct ggcctgtgat cgagcggcaa actgcgacga agccggtacg actggcttct
     3421 gccagatctg ccggcaaccg caacaggccg ggtgtcacta ccggcctgtt gctgctgacg
     3481 ctggtggcat tgggtggcgg ctggtactgg cagtcgacga aagtggctgg caagcacggt
     3541 gacgagaagg gcgtacctgc atccactgcg agcatggcgc aggaaaccag tgtcgtgctt
     3601 ctgacgacac ccacggaggc gatcgcagac gatactgcgc caaccgatgt gcagcaggcg
     3661 ttgtcggata cgtccctcgc cggcctcgag atccccaaca gtctgatgat ggacggcgat
     3721 cagctggtcg ccaacgaagg gctgcgcgag ttgatggatt tctttctcac cctcaccggc
     3781 gagcgcgaac cggcccagat tcgcgcgctg tttgcagccg ccgccaccgc gcagtgtgca
     3841 gcgatttgcg cggaacaggc gttggtgatt tacgatcact acctggaata tctgcgggcg
     3901 atggagacgg ccagtgctga tctgcaagat actgatgatc tgcgcagccg attgcagcgg
     3961 gttgccggca tccggtcgga attcctgggg gaggaggtcg ccaagagcct gttcgcggcg
     4021 gagaacgcct atgacaacct gcgcgtcgca cagtgggaaa tccgtactca aaccgatctg
     4081 tccgccgcgg aaaaaagcga ggcgctggca gcactggaga gcagcctgcc accggatctt
     4141 gcagcccgcg agcaggagag aaaattgctg cgctcggtgc gaaacctgaa ccggacactg
     4201 gacgaacagc aaaaggatgc agcagaccgg tatgctgccc gcgccgcgct gctggatgtg
     4261 gccgctgccg aacgactgca ggcgctggat acccgtcgcg ccgaatgggc acaacggtat
     4321 gaggcgtacc ggcaggaacg cacccgcctg gacatcagcg ggttggacac ggtggatcgc
     4381 cagcaggcac tggaacaact gcggctgcgg catttttcgg ctcaggaaag tcagcgcgcg
     4441 gcggcactgg atcgcgtcaa cggtctggaa cgctgaaacg cccagaccgg ttcagtgccc
     4501 tgccctcgtg gccatccctt ctgcggcaat gccacggcga aatcgccctt ttcagtgcgg
     4561 ccggacattg attatccgca cgatatcttc gcccgcaaac gcctgcacgc cgaattctgg
     4621 cttgagcttg ccggtaacac gcagcgcggt cacctgcccg ttgtagatgg agcccaatga
     4681 cgccagcagc gcctgttcat cgaaagccag caccagatcc agacggccgt cggaaccgtt
     4741 cgcggtacaa tccgatacgt tgactttacc tacataggga agcaccggcc gcgtggcatg
     4801 gcgaatggac caggcttgcg ctggtacacc attgatccgc accgattccg ggtcgatccg
     4861 gttcacgttg aacgccgtgg taccggcaat ggcgacaggt acccgtcccc gcccgtgagc
     4921 attgagtgga ttgggacagg tggagggcct gaaatccgcc gacaccagag tggtgtgacg
     4981 tccactgacc gtcatacccg acagtgaaaa acgcccgttg accgcaggat cattggtcat
     5041 gtctccaaac gccaggcgcg gatgcagctt gaaatcggcg cgctgcaacg cgagttgccc
     5101 atcggcataa aaccgggcca tgccgtcgct ttccacttcg atccggtaat gatggaagtc
     5161 atcccgggtg ttgaacggga agcgctctga ctcatcgccc cagccaatct catccgcatc
     5221 gaaataaatg aactgcgcac gtggactgcc ggtgaatgaa ctgagccggg ggtcctgggt
     5281 ggaggcgtag aacacgatgg ccgaatcaaa tgca
//
LOCUS       DASUEG010000965         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_345243, whole genome shotgun sequence.
ACCESSION   DASUEG010000965 DASUEG010000000
VERSION     DASUEG010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345243"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1139
                     /locus_tag="VFX11_18150"
     CDS             336..1139
                     /locus_tag="VFX11_18150"
                     /inference="COORDINATES: protein motif:HMM:NF014514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit A"
                     /protein_id="HEX4940601.1"
                     /translation="MNELQLEESRAKEAKETRAQILDMESLNNPAAMKLYRRLDGILI
                     LVLFTFLTLGIQIQFALTAGDWDYWIDWRDRRWWPLVSPISLLMVIGAFTYGIWQRVR
                     LPIIATAITVLLCLVSWGSRYLNFHQFTYFPLTFTFPSTYIALGILLDCSLIMTRSII
                     VSAVIGGGLFGALVYPLNWPLFAAYRIPVELNGMLLSLGDLMGYEYIRTTTPEYLRII
                     EHSTLRTFGGSVTPLTAVFAGFVSIVVYALFLWIGSYVNKLAVFVKRIV"
     gene            1276..>2067
                     /locus_tag="VFX11_18155"
     CDS             1276..>2067
                     /locus_tag="VFX11_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HEX4940602.1"
                     /translation="MMLAGDVLAHGERAQLASMRMRTVHWFDTQVSPTKAKVGDTVTI
                     TGKFVPSMYWPDTLPSIGATAFLNVGVPGPTFLRVHSEVNGVPMMRSTAFPLGKLYEY
                     KVVLRARIPGRYHVHPLINVLDAGSLVGKGTWLEVDKADSDAPFVNNTTTLTGQTVDL
                     ETHGLRDVQLWHLLWIAMGVAWLLFWLIRKELFIPRFMRIRALGDDRANELLTKRDFV
                     VSAMFFTLTLAIILTGYLWAERKYPVTIPLQTGKVVVEGLDVPMGD"
BASE COUNT          399 a          519 c          583 g          566 t
ORIGIN      
        1 tttacgcaga agagttgttt gctgcgccga tccactgggg ttttgtgttg ctgggctggt
       61 cactgttctt ctttgtgccg ctgctcgtgc agctcttcac ttacatgggt cgctccatct
      121 cccaggtagc ggcgctgaga agccggcaga tggtttgagt cgtcctttca caagatcatt
      181 tgcttcgccc cgatagggtt ctctgtcgaa gcagtggttc tcgaacgagg cggtttcgca
      241 gtccccgtaa gcctgcttgc ggggactgtc tttctgagaa cggccgttgc gcacccgcag
      301 ttcactgatt ttcgaaaata cgaggtgttg cccagatgaa cgaattgcaa ttggaggaat
      361 ctcgggccaa agaggccaag gaaaccaggg cccagatcct tgacatggaa tcgctcaaca
      421 acccggctgc gatgaaacta tatcgacggt tggacggcat cctgattctg gttttattca
      481 cttttttaac gttggggata cagatccaat ttgcccttac agccggcgat tgggattatt
      541 ggattgactg gcgtgatcgc cggtggtggc ctttggtgtc gccgatttcc ctgctgatgg
      601 tcattggggc atttacctac ggtatatggc agcgtgtgcg tctgccaatc attgctaccg
      661 ccataaccgt gctgctgtgt ctggtgtcct ggggtagtcg ctatctgaat tttcaccagt
      721 tcacttactt cccgctgacc tttacctttc cgtcaaccta tatcgcgctc ggcattttgc
      781 tcgattgttc attgatcatg acgcgtagca ttatcgtttc agcggtgatt ggcggaggct
      841 tgttcggcgc gctggtgtat ccgttgaact ggcctttgtt tgctgcctac cgaatcccgg
      901 tagaactcaa cggcatgttg ctgtctctgg gggatttgat ggggtacgaa tatattcgta
      961 ccacgactcc cgaatacctg cgcattatcg agcactctac tttgcgaacg tttggcggtt
     1021 cggtcacgcc attgaccgcc gtatttgcgg gtttcgtcag cattgtggtt tatgcccttt
     1081 ttctttggat cggttcttat gtgaacaagc tggcagtttt tgtcaagcgc atcgtttgag
     1141 caaatgacag ccaagcttca ccaaataaac gggcgatatg agggggcgta gggcgtgggg
     1201 tctacaaaaa acatgatagc gacaggaaag gttcattcaa ggctcgggtt ggtatcgact
     1261 gcggcgttgg tgctgatgat gcttgccggc gatgtgctcg cccacggcga gcgtgcccag
     1321 ctggccagta tgcggatgcg caccgtgcat tggttcgaca cgcaggtgtc acccaccaag
     1381 gccaaagtag gcgataccgt cactatcacc ggcaaattcg tcccgtccat gtattggccg
     1441 gatacgttgc ccagcattgg cgccactgcg tttctgaacg ttggtgtacc cggcccgact
     1501 tttctacggg tgcattcgga agtgaacggc gttccgatga tgcgctctac cgcctttcct
     1561 ttgggaaagc tgtatgaata caaggtggtg ctgcgtgcgc gcatcccggg tcgctatcat
     1621 gttcatccgc tgatcaatgt gttggatgcg gggtccctgg ttggcaaagg aacctggctc
     1681 gaagttgaca aggcagattc cgatgcgccc ttcgttaaca atacgaccac gctaaccgga
     1741 caaaccgtcg atctagaaac ccatggtctg cgcgatgtgc agttgtggca tctgctctgg
     1801 atcgcgatgg gcgttgcttg gctgttgttc tggctgatcc gcaaggagct gttcattccc
     1861 agattcatgc gcatcagggc cttgggtgac gatcgcgcca atgagctact gacaaaaagg
     1921 gattttgttg tttcggcaat gttcttcacc ctgacgctgg caattatcct taccggatat
     1981 ctgtgggctg aacggaaata cccggtgacc attccacttc aaaccggcaa agtagtcgtt
     2041 gaaggtttag atgtgccgat gggtgat
//
LOCUS       DASUEG010000966         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1860869, whole genome shotgun sequence.
ACCESSION   DASUEG010000966 DASUEG010000000
VERSION     DASUEG010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860869"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFX11_18160"
     CDS             complement(<1..861)
                     /locus_tag="VFX11_18160"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX4940603.1"
                     /translation="MMNVRIVSTGLYLPARVQTSSELSGLIGRSEDWIISRTGVRERR
                     IAEEPMNSMAARAALQAIGDGPRPDCIINASLTPLQLIPDSSVFIQRALGWESIPCWS
                     IHATCLSFLVALMNASALIHADLYRRILVVSAEAGTPWRNLNQPESAALFGDAAAAVV
                     LEKASETSAILDWEMSTWPDGAELTEYRGCGTRKPPGHPDSIPEDNLFNMMGPKVFRL
                     VLMHISSVLRALFERNDMTFDDVDLFILHQASGPGMEGFVKLGFAREKIVNIVAEYGN
                     CIAASMPMTLA"
     gene            complement(962..1153)
                     /locus_tag="VFX11_18165"
     CDS             complement(962..1153)
                     /locus_tag="VFX11_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940604.1"
                     /translation="MSTRTTVIAANENGASKNFREPIVARLSPLHFHLGRVQRIFVAY
                     QFIRAKKLSVLSGMDSYKK"
     gene            1466..2608
                     /locus_tag="VFX11_18170"
     CDS             1466..2608
                     /locus_tag="VFX11_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4940605.1"
                     /translation="MAGPLSGIRIIELAGIGPGPFCGMMLADMGADVIRVERPGGNPG
                     DAGGHDILFRNRKSVAVNLKTPAGVETVLRLCEKADGLFEGFRPGVAERLGVGPQVCL
                     ARNPKLVYGRMTGWGQTGPLAKAAGHDINYISLSGALHAMGRRGEGPVPPLNLVGDFG
                     GGGMMLAFGMVCGILEASRSGKGQEIDCSMVEGASALMAMFYGLHGIGQFSDERGTHM
                     LDSGAHFYDTYATADGKYISIGSIEPQFYALLKQQLQLDESVFGEQLNPQRWPQQKEK
                     LAAIFRTRTRDEWCKLMEGTDVCFAPVLGLEEAPRHPHNLARNSFVPVGDMLQPAPTP
                     RFSRTPSGTPRPISAPGTDTRAVLDDCGFSSAEIDALLANGAVAQV"
     gene            complement(2727..3593)
                     /locus_tag="VFX11_18175"
     CDS             complement(2727..3593)
                     /locus_tag="VFX11_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012533334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX4940606.1"
                     /translation="MKNIWAIVAMLFVFAFTATEAQAKKFGGSKSFGRSYQTAPAPAA
                     KPDAPTQTAAPGAAAAAGKRGMLGGLMGGLLAGGLIAALIGGGAFEGIQFMDILIIGL
                     LAFVIFKLLRSRRPVASSPAPVYAGGQGGNNSERRAFDFENKSSGGAVFTPAGSGFGV
                     SQVPFNLPAGFDLTAFLAGARDHYRTLQDAWNKNDLVKIQEYVSPELYNDLRAERATL
                     VGDQHTEVLFVDAELVRADQIGSRAEVSVKFSGRYRDTVEGVEEPITDIWHLERDTAQ
                     AGSPWYIVGIEA"
     gene            complement(3633..>4769)
                     /locus_tag="VFX11_18180"
     CDS             complement(3633..>4769)
                     /locus_tag="VFX11_18180"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4940607.1"
                     /translation="SCRCFLFGDARLCGAGSVPVWCWFSSALAANRLIGDDMDIVNYS
                     TGIFVRALDKLYRSRGLDFSVLLRRKGFPAASAMTVETPITVRLTTDLLNAAMEELQD
                     PVLGISLSRFADYPHFGALGLSLAAGGTIQESLHRVARYHRLVSDVVTPQVLYLPQQI
                     ALKFAPSGGHVPHPQALLFVLASFVRLLRARLQERFDPAQIQVPAGMESAHDALRHFF
                     RCPLRSGDAFVLVLHRADEDRLLGSGDSELCDLIEFTLRQRLRQCNQGQFIAALGKWI
                     AERLPAGEPTLAEAAENFQLGVRTLQRKLKEEGVTWLQLVETARRELVERYRGVEGVT
                     VTQLAFLAGYQDVSSFSRAFKRWYGVAPTQMASSGPVVFPVQST"
BASE COUNT         1027 a         1465 c         1356 g          921 t
ORIGIN      
        1 ggcgagcgtc attggcatgg aggcggcaat gcagttgcca tactcggcga cgatgttgac
       61 aattttttcg cgcgcgaaac cgagcttgac gaaaccttcc atgccaggcc ccgatgcctg
      121 atgcagaata aacagatcga catcatcaaa tgtcatgtca ttgcgctcga acaaggcgcg
      181 caaaacgctg gaaatatgca tcagcaccag acggaacacc ttcggcccca tcatgttgaa
      241 cagattgtct tccggaatac tgtcgggatg gcccggcggc ttgcgggtgc cgcaaccacg
      301 gtattcggtg agctctgcac cgtccggcca ggtggacatt tcccagtcca gaatcgcgct
      361 ggtttcactg gctttttcga gcaccaccgc cgccgcggca tcaccgaaca gtgcggcact
      421 ttccggctgg ttcaggttgc gccagggcgt acccgcttct gccgacacga caagaatgcg
      481 ccggtacaga tccgcatgga tcagcgccga cgcgttcatc agtgcgacca gaaacgacag
      541 gcaggtggca tggatggacc agcagggaat gctttcccag cccagcgctc gctgaatgaa
      601 aacagaactg tcaggaatca gttgcagtgg cgtcagcgat gcattgatga tgcagtcagg
      661 acggggacca tcgccaatgg cttgcagtgc agccctggcc gccatgctgt tcatcggctc
      721 ttcggcaatg cgacgctcgc gcacgccggt tcgggaaatg atccagtcct cacttcgtcc
      781 gatcaggcca gaaagctcgg aagacgtttg aacacgcgca ggcaaatata aaccggtgga
      841 tacgattctg acgttcatca ttcgagaatt ccttttctct tttatgaaat cgcatgtgct
      901 aaaactatat aaaaccgtgc cggctcacaa cccggaatat gtggcaattg tctgcagtac
      961 gttatttttt gtaagaatcc atccctgata aaactgacaa tttttttgca cgaataaatt
     1021 gataggcaac aaaaatgcgc tgtacccgcc ctaagtgaaa atgcagcggc gacaaacgtg
     1081 caacgatcgg ttcgcggaaa ttttttgaag ctccattttc attggctgcg ataaccgttg
     1141 tgcgtgtcga cataccaatt ggagcgctgc ttcactgcac gaaaaaatcc agggctgaag
     1201 caccgtattc aggccatgat ttcacgccat tcacagaatc gcgcacccac atcgtctttc
     1261 acttcctggc ggggttgtgg tcgcaacctg tgatcgcgtc gggccgtggc tccatgaccg
     1321 ttaccgggcg ccaatagtgc cttgcttttc tgcacctgca ctgctgctgg caaggctgga
     1381 cttccgcccg gacagccttt acctttcagc ctgcgaccta ccggtcatcc actattctta
     1441 gttccccgat cttcaaggag ttcccatggc aggccccctc agcggcatac gcatcatcga
     1501 actggccggc atcggccccg gtcccttctg cggcatgatg ctggcggaca tgggcgcgga
     1561 cgtgatccgg gtggaacggc ccggtggcaa tcccggtgac gccgggggcc atgacatcct
     1621 gttccgcaat cgcaaaagtg tggcggtgaa cctgaaaaca ccggccggcg tggaaaccgt
     1681 gctgcgcctg tgcgaaaaag ccgatggtct gtttgaaggt ttccgcccgg gcgtggctga
     1741 gcggctgggc gtgggaccgc aggtgtgtct ggcacgcaat ccgaagctgg tgtacggccg
     1801 catgacgggt tggggacaga cgggtccgct ggcaaaggca gcggggcatg acatcaatta
     1861 catcagcctg tccggcgcac tgcatgccat ggggcggcgc ggtgaaggcc cggtgccacc
     1921 gctgaatctg gtaggcgatt tcggtggcgg cggcatgatg ctggcattcg gcatggtgtg
     1981 cggcattctg gaagcgtcac gctcgggcaa gggacaggaa atcgattgtt cgatggtgga
     2041 aggcgccagc gcgctgatgg cgatgttcta tggtctgcac ggcatcggcc agttcagtga
     2101 tgaacgcggc acccacatgc tggattccgg cgcgcatttt tacgatacct atgccaccgc
     2161 cgacggcaaa tacatcagca tcggcagcat cgagccgcag ttctacgcgc tgctgaagca
     2221 gcagttgcag ctggatgaat cggtgtttgg cgaacagttg aatccgcagc gctggccgca
     2281 gcagaaagaa aaactggcgg cgattttccg cacccgcacc cgcgatgaat ggtgcaagct
     2341 gatggaaggc accgatgtat gcttcgcgcc cgtgctgggg ctggaagaag cgccacgcca
     2401 tccccacaat ctggcccgca acagtttcgt gccggtgggc gacatgctgc agccggcacc
     2461 gacgccgcgt ttcagccgta cgccctccgg caccccgcgc ccgatcagtg cgccgggtac
     2521 cgatacccgt gcggtactgg acgactgcgg attttccagc gctgaaattg acgcactgct
     2581 ggcaaacgga gccgtggcgc aggtttaaaa aaaccggggc cataaaatag ggcgccataa
     2641 aaaaagcccg gcggttggaa tgaccgccgg gctttttttc gacttccgta ttttcagtgc
     2701 tttccgcaac ctacacgccg ttgcgattat gcttcgatac ccacgatata ccagggcgaa
     2761 cccgcctggg ccgtgtcacg ctccaggtgc cagatatcgg tgatgggttc ttccacacct
     2821 tccaccgtat cacggtagcg gccgctgaac ttcacgctca cctccgcacg gctgccgatc
     2881 tggtcggccc gcaccagttc cgcatccaca aacagcactt cggtatgttg atcgcccacc
     2941 agggtggcgc gctcggcccg caaatcgttg tacagctcgg ggctgacata ttcctggatt
     3001 ttcaccagat cgttcttgtt ccaggcgtcc tgcagggtgc ggtagtgatc acgggcaccg
     3061 gccagaaatg cggtcaggtc aaaaccggcc ggcagattga acggcacctg gctgacaccg
     3121 aagccgctgc cggccggtgt gaacaccgcg ccaccggacg atttgttctc gaaatcgaag
     3181 gcgcgccgct cactgttgtt gccgccctgc ccaccggcgt agaccggtgc cgggctgctc
     3241 gccacaggac gccggctgcg caacagcttg aagatcacaa acgccagcag gccgatgatc
     3301 aggatgtcca tgaactggat gccctcgaag gcaccaccgc ctatcagcgc tgcaatcagg
     3361 ccacccgcca gcagaccgcc catcagacca ccgagcatgc ctcgcttgcc cgcagcagct
     3421 gcggcgccgg gcgctgcggt ctgggtagga gcatccggct ttgcggccgg cgccggcgcc
     3481 gtctggtagg aacggccgaa ggatttgctg ccaccgaatt tcttggcctg cgcctcggtg
     3541 gcggtaaagg cgaacacaaa caacatggcg acaattgccc atatattttt catggcttta
     3601 tcctttccat tgatgacaca actgtcgaca tattatgtcg attgcaccgg gaagacaacc
     3661 ggtccggaag acgccatctg cgtgggcgcc acaccgtacc agcgcttgaa ggctcgcgaa
     3721 aatgaactga cgtcctgata gccggccaga aacgccagct gggtcaccgt cacaccctcc
     3781 acgcctcgat agcgttccac cagttcgcgc cgcgcggttt ccaccagctg caaccaggta
     3841 acaccttcct ccttcagctt gcgttgcagg gtccgcacac ccagctggaa attctctgcc
     3901 gcctcagcca gcgtgggctc gccagccggc agccgttccg ctatccattt gccaagcgcc
     3961 gcgatgaact gcccctggtt gcattgacgc aagcgctgac gcagggtgaa ttcgatcagg
     4021 tcgcacagct cgctgtcgcc gctgccgagc aaccggtcct cgtccgcgcg gtgcaaaaca
     4081 agcacgaagg catcaccact gcgcagtggg cagcggaaaa aatgccgcag ggcatcatgc
     4141 gcggattcca tgccggccgg cacctggatc tgcgcgggat caaagcgttc ctgcaaccgc
     4201 gcccgcaaca gacgcacaaa gctggccagc acaaacagca aagcctgcgg atgcggcaca
     4261 tgaccgccgg acggcgcgaa tttcaatgca atctgctgtg gcagatacaa cacctgcggt
     4321 gtcaccacat cactcaccag tcgatgatag cgtgcgacac ggtgcaatga ttcctgaatc
     4381 gtgccacccg ccgccagcga caatcccagc gcaccgaaat ggggatagtc ggcaaaacga
     4441 gacaggctga tcccgaggac agggtcctgc agttcctcca tcgctgcatt cagcaggtcc
     4501 gtggtcaaac gcacggtgat gggggtctcc actgtcatcg ccgatgcggc gggaaatccc
     4561 tttcgccgca gcaagacaga aaaatccagt ccccggctgc gatacagctt atccagcgcc
     4621 cgcacaaaaa taccggtgct gtaattcacg atgtccatgt cgtctccaat caacctgttg
     4681 gcagccaatg ccgagctgaa ccagcaccac accggtacgc ttccggcacc acacaaccgt
     4741 gcatcaccaa acaggaagca ccgacatga
//
LOCUS       DASUEG010000967         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_433680, whole genome shotgun sequence.
ACCESSION   DASUEG010000967 DASUEG010000000
VERSION     DASUEG010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433680"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFX11_18185"
     CDS             complement(<1..1097)
                     /locus_tag="VFX11_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX4940608.1"
                     /translation="MSQLSAETKTPLVLSISDRVPNLVILLVLIFLIFARTGESIHGQ
                     MTKLGEYLWEDYFSLRAEVPMPTCDRNPNIAQQLDQLEAEAASASADFDLLDEGFNRA
                     EAEASLQNQKRLCALEYQQAEAYESRVTPSVKAFRSLESVFSQASVFATAQQQSFLLI
                     LLFFAAGIATLQRHHIAFRPIEWRLDFQVSTSLQLLANTSLALSAWLYRDSTFNSSID
                     VADPFLVNGLVAGTTVLALISLYQLFNIPADARRRGKLGRALLSVPLYTYVMLAFAII
                     VFFVQQHPAGLSIYFSAFFDNAGSYLDVALYLWCGMLLKQTQLGERVFNIFTPWKLPP
                     ELLATVAILVMAVPTAYTGASSIIILAAGVVV"
     gene            complement(1130..2161)
                     /locus_tag="VFX11_18190"
     CDS             complement(1130..2161)
                     /locus_tag="VFX11_18190"
                     /inference="COORDINATES: protein motif:HMM:NF039651.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative solute-binding protein"
                     /protein_id="HEX4940609.1"
                     /translation="MKSTSLKRLIQQSLTGMLTCVALAASAYAAEPVPLTMCAFTFMG
                     EGGPEHQALLDYQTAALGWGVKLTMKPYINEKIVAEELKSGVCDLANMTSNQARNFNK
                     FTGTLDSPAAIPSYKHLRIVLETLAKPTAAKYMQVGDYEVVGIQPAGEVFLFTNDRKL
                     QSLSDMAGKRIAILDTMPEMRQLVLDLGMTPVSSTMTNMYQKFNNGATDITAGPAVVY
                     DLMELHKGLKPNGGILNMPLVQGNNQFVARSSKLPPGFAQKSREYFVNNFDKNLSMIQ
                     SAEQKIPKELWVPLPESRKDEYDQQTRNIRLAFRDKSIYDAKMLTLLRKVRCQVDGTL
                     AECTAQGAE"
     gene            complement(2231..>3149)
                     /locus_tag="VFX11_18195"
     CDS             complement(2231..>3149)
                     /locus_tag="VFX11_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940610.1"
                     /translation="GMVSFGNDYITPWFMASDDTDVMCAMGEGMGAMTFPLGPATDPM
                     VPMLTLASGMCADERSKEEELRYIRAMRKNDVEEAQDARTLQKRWAELAARRQYFGYQ
                     ATVRHFGEPGSECPEFEDRNEEMSYLFGLLGGLQALQTDMNNGGTAGVALDVMPKVVK
                     GLDCVNSEEFWGIPDAVQASVNVIKANVGGDRAAVDAGLKELRAASDRGKSQGVRMVQ
                     LMEAAVFNTMGDTEKAKAVIRDHVASKKQTAPNPELKIIDEMATRGIRLISDKMWTQA
                     TGQRTPYNQLGTFWDDRSESSALDIDELL"
BASE COUNT          650 a          902 c          892 g          705 t
ORIGIN      
        1 accaccacgc cagcagccag aatgatgatg gatgatgcac cggtgtaggc cgtgggcacc
       61 gccatgacca gaatggcaac agtagccagc aattccggtg gcagcttcca gggcgtgaag
      121 atattgaaga cgcgttcacc cagctgggtt tgcttcaaca gcatgccgca ccagaggtac
      181 aacgctacat ccaggtagga acctgcatta tcgaaaaatg cgctgaagta aatcgacagt
      241 ccggccgggt gttgctggac gaagaatacg atgatagcga aggccagcat cacataggtg
      301 tacagcggca ccgacaacag cgcgcgcccc agcttgcctc gacggcgggc gtccgctgga
      361 atattgaata gctgatacag ggaaatcagc gccagcaccg tggtacccgc caccagaccg
      421 ttcaccagga atggatccgc cacatcgatg ctggagttga acgtactgtc gcggtatagc
      481 caggccgaca gggccaggct ggtgttggcc agtaattgca atgacgtgga gacctggaaa
      541 tccaggcgcc attcgatggg ccggaacgca atgtggtgac gttgcagcgt ggcgatgccg
      601 gcggcgaaaa acagcaaaat cagcaggaag gattgctgct gggccgtcgc gaatacgctc
      661 gcctgcgaga acacactttc cagcgaacgg aaggctttga cagacggcgt cacccgactt
      721 tcataggctt ccgcctgctg atattccagt gcgcacaaac gtttctggtt ctgcagcgaa
      781 gcttcggcct ccgcccggtt aaatccctca tccagcagat cgaaatcagc ggatgcactg
      841 gcggcctccg cttccagctg gtccagctgc tgcgcaatat tgggattgcg atcgcaggtc
      901 ggcatgggca cttccgcccg cagggaaaaa taatcctccc acaggtattc gcccagtttg
      961 gtcatttgcc catggatgct ttcaccggta cgggcgaaga tcaggaagat caacaccagc
     1021 aggatgacca gattgggcac gcgatcggag atcgacaata ccagcggtgt cttggtttcg
     1081 gcggatagtt ggctcacgtc ggattacctg tcaggatgcg cgttacgcgt tattcggcgc
     1141 cttgcgccgt gcattcggcc agcgtgccat ccacctgaca gcgcaccttg cgcagcaggg
     1201 tcagcatttt ggcgtcataa atgctcttgt cgcgaaacgc cagacggata ttgcgggttt
     1261 gctggtcgta ttcatccttg cggctttccg gtagcggtac ccacaattcc ttgggaatct
     1321 tctgctccgc actctggatc atgctcaggt tcttatcgaa attattgaca aagtattcgc
     1381 gggatttctg tgcaaaaccc ggcggcaatt tgctcgaacg agcgacaaac tggttattgc
     1441 cctgcaccag cggcatgttg agaataccgc cgttgggctt cagccccttg tgcagctcca
     1501 tcaggtcata caccactgcc gggccggccg ttatgtcggt cgctccgttg ttgaatttct
     1561 ggtacatgtt ggtcatggtg gatgacaccg gcgtcatgcc gaggtccagc accagctggc
     1621 gcatttccgg catggtgtcc agaattgcga tgcgcttgcc cgccatatcc gacagactct
     1681 gcagcttgcg atcgttagtg aacagaaaca cttcgccagc aggctgaatt cccaccactt
     1741 cataatcacc cacctgcata tatttggcgg ccgtgggctt ggccagcgtt tccagcacga
     1801 ttctcagatg cttgtaggac ggtatggctg ccggggaatc cagcgtgccc gtgaacttgt
     1861 tgaaattgcg cgcctggttg ctggtcatgt tggccaggtc gcagactccg gatttcaatt
     1921 cttccgcaac gattttttca ttgatgtagg gtttcatcgt cagtttgaca ccccagccca
     1981 gtgcagcggt ctggtagtcc agcagcgcct ggtgttccgg gccgccctca cccatgaacg
     2041 tgaaggcgca catggtcagc ggcacgggtt ccgctgcata ggcactggcg gccagtgcga
     2101 cgcaggtcag catgccagtc aaagactgct ggataaggcg tttgagcgaa gtggatttca
     2161 tggtcatatc ccttgtagtg ttgttgtggc ccatttcacg gctgggcggg aagtgattag
     2221 agatggcggt ttaaagcaat tcgtcaatat ccagcgcgga cgattcactc ctgtcatccc
     2281 agaacgtccc cagctggttg tagggcgtgc gctggccagt ggcttgtgtc cacatcttgt
     2341 cggaaataag gcggatgccg cgggtggcca tttcgtcgat gattttcagc tcgggattgg
     2401 gcgcggtttg cttttttgaa gccacatgat cccgtatcac ggctttggct ttttcggtat
     2461 cgcccatggt attgaatacg gcggcttcca tcagctgcac catgcgcaca ccctgggatt
     2521 tgccgcgatc tgaagccgcg cgcagttcct tcaggcctgc atcaacggca gcgcgatcgc
     2581 cgccaacgtt ggccttgatg acattgacgg aggcctgcac cgcgtcaggg atgccccaga
     2641 attcttccga attcacacaa tcaagcccct tcaccacttt cggcatgaca tccagtgcca
     2701 cgccggccgt gccgccgtta ttcatgtcgg tctgcagtgc ctgcaatccg cccagaaggc
     2761 cgaacagata actcatttct tcattgcggt cttcgaactc cggacattca ctgccgggtt
     2821 caccgaagtg gcgcactgtg gcctgatagc cgaagtactg tcggcgagcc gccagctcgg
     2881 cccaacgctt ttgcagcgtg cgggcatcct gcgcttcttc gacatcgttt ttgcgcatgg
     2941 cacggatata acgcaattcc tcttccttgc tgcgctcgtc agcgcacata cctgacgcca
     3001 gcgtcagcat gggcaccatg ggatccgtag cggggccgag tggaaacgtc atcgcgccca
     3061 tgccttcgcc cattgcacac atgacatcgg tgtcatcgct agccatgaac cagggagtga
     3121 tgtagtcgtt gccgaaactc accatgccg
//
LOCUS       DASUEG010000968         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1205851, whole genome shotgun sequence.
ACCESSION   DASUEG010000968 DASUEG010000000
VERSION     DASUEG010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205851"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /gene="scpA"
                     /locus_tag="VFX11_18200"
     CDS             <1..1942
                     /gene="scpA"
                     /locus_tag="VFX11_18200"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX4940611.1"
                     /translation="ATMYAGRPWTIRQYAGFSTAAESNAFYRKNLAAGQQGASVAFDL
                     ATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDTVSVSMTMNGAVLPVLAM
                     YIVAAEEQGVSQDKLSGTIQNDILKEFMVRNTYIYPPEGSMRIIADIIGYTSKFMPKY
                     NSISISGYHIQEAGADAALELAFTLADGKEYIETALKAGLDIDHFAPRLSFFFGIGMN
                     FYMEIAKLRAARLLWDRIVKEYNPKKDESRMLRTHCQTSGWSLTEQDPYNNVVRTTIE
                     AMAAVFGGTQSLHTNAFDEAIALPTEFSARIARNTQIIIQEETGITKVIDPWGGSYMM
                     EKLTQDIADKAWGIIEDIQARGGMAKAIEQGEPKLRIEESAARKQARIDRGEDVIVGV
                     NKYVLKKEDDIDVLEIDNSSVREQQIARLKQMRATRDQKACQAALDALTEWARTGEGN
                     GLDLAIKASRARASVGEISDAIEKVWGRYNANSKTVSGVYGSSYENDSDWTEMQSDIK
                     AFEDRHGRRPRMLVAKMGQDGHDRGAKVIATAFADVGFDIDLSPLFSTPSEVAKQAIE
                     NDVHVIGVSSQAAGHKTLVPDLINALKAQNATDIIVVVGGVIPRQDYDFLYQSGVKGI
                     FGPGTKIPLAARKVLDAINVSA"
     gene            1949..2914
                     /gene="meaB"
                     /locus_tag="VFX11_18205"
     CDS             1949..2914
                     /gene="meaB"
                     /locus_tag="VFX11_18205"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX4940612.1"
                     /translation="MSIDKEALLQGNRRALAKAITLTESKLDSHRQEAQELLESLLPH
                     TGNSIRIGITGIPGVGKSTFIEAFGLHLIRAGKKVAVLAVDPSSPITGGSILGDKTRM
                     EVLSQQENAFIRPSPSGGALGGVAQKTRESMLLCEAAGYDVILVETVGVGQSEYEVAS
                     MVDFFLVLMLPNAGDELQGIKKGILELADAIVINKADGSARAMADQARHHYNNALSIL
                     HHHHFWQPRVLLCSAMENKNIDVIWEMILEYRQRSQEAGVFLTKRARQNQEWMWRLVH
                     ELINRSLSHDPHAKAVCDAMQNKVNAGQTTPYIAAHRIVESLRQI"
     gene            3089..>3763
                     /locus_tag="VFX11_18210"
     CDS             3089..>3763
                     /locus_tag="VFX11_18210"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX4940613.1"
                     /translation="MGTKMMSDTEQRGGNVSSVAATLPSLVKQVKDNTFAILSNNFSH
                     FFSSCDDLFFDLASKAGSNKEQNLYFDSMREVRMKKPQVWSLFKDGYEENFRNLTKSR
                     LSGKKKSAPEPLNLDTMQLVDKEDMEQDVAITGIVNKARTENQEQLYQLNCRFDYLLP
                     DVKVNEGNNPLDPAQICNAVSKALRVLDLDIRCKVILLKHLDRTVIVELRNIYSLANE
                     LMINAGV"
BASE COUNT          899 a         1159 c         1018 g          687 t
ORIGIN      
        1 agccaccatg tacgccggcc gcccctggac catccgccag tacgccggct tttccaccgc
       61 cgctgaatcc aacgccttct accgcaagaa cctggctgcc ggccagcaag gcgcttccgt
      121 ggcattcgac ctggccaccc accgcggcta cgactccgat cacccgcgcg tgaccggcga
      181 tgtcggcaag gccggggtgg ccatcgattc tgtggaagac atgaaaatcc tgttcgacgg
      241 cattccgctg gacaccgtct ccgtctccat gaccatgaac ggtgcggtgt tgccggtact
      301 ggccatgtac attgtcgccg ccgaagaaca gggcgtgtcg caggacaaac tgtccggcac
      361 gatccagaac gacattttga aagaattcat ggtgcgcaac acctacatct acccgccgga
      421 aggctccatg cgcatcattg ccgacatcat cggctacacc tcgaagttca tgccgaagta
      481 caactccata tccatttccg gctaccacat ccaggaagcc ggtgccgacg cggccctgga
      541 actggccttc accctggctg acggcaagga atacatcgaa accgcgctga aagccggcct
      601 cgatatcgac catttcgcgc cacgcctgtc gttcttcttc ggcatcggca tgaatttcta
      661 catggaaatt gccaaactgc gcgctgcgcg tctgctgtgg gaccgcatcg tcaaggaata
      721 caatccgaag aaagacgaat cccgcatgct gcgcacccat tgccagactt ccggctggtc
      781 gctcaccgag caggacccgt acaacaacgt ggtgcgcacc accatcgaag ccatggcagc
      841 cgtgttcggt ggcacccagt cgttgcacac caacgcgttt gacgaagcga ttgctctgcc
      901 caccgaattt tccgcccgta tcgcccgcaa cacccagatc atcatccagg aagaaaccgg
      961 catcaccaag gtcatcgatc cgtggggcgg ttcctacatg atggaaaaac tgacccagga
     1021 catcgccgac aaggcctggg gcatcatcga ggacatccag gcccggggcg gcatggccaa
     1081 ggccatcgaa cagggtgagc cgaaactgcg catcgaggaa tccgctgcac gcaaacaggc
     1141 acggatcgat cgcggcgaag acgtcatcgt cggcgtcaac aaatacgtat tgaagaaaga
     1201 agacgacatc gacgtgctgg aaatcgacaa ctcgtcggta cgtgaacagc aaattgcccg
     1261 actcaagcag atgcgcgcca cccgcgacca gaaggcctgc caggctgccc tggacgcgct
     1321 caccgaatgg gccagaaccg gcgaaggcaa cggcctggat ctggccatca aggcctcccg
     1381 tgcccgtgca tccgttggcg agatcagcga cgccatcgaa aaagtctggg gccgctacaa
     1441 cgcgaacagc aagaccgtgt ccggcgtgta tggcagctcc tacgaaaacg attccgactg
     1501 gaccgaaatg cagtccgaca tcaaggcgtt cgaagacagg cacggccgcc gtccgcgcat
     1561 gctggtggcg aaaatgggcc aggacggcca tgaccgtggc gccaaagtga tcgccaccgc
     1621 gtttgctgac gtgggcttcg acatcgacct gtcaccgctc ttctcaacac ccagcgaagt
     1681 cgcgaaacag gctatcgaaa acgacgtgca cgtgatcggc gtatcgtccc aggccgccgg
     1741 ccacaagacg ctggtacccg acctgatcaa cgccctgaaa gcccagaacg ccacggatat
     1801 catcgtggtg gtaggcggcg taatcccgcg tcaggactac gatttcctgt accagtccgg
     1861 cgtaaaaggc atcttcggcc ccggcaccaa aatcccgctg gcggcccgca aggtactcga
     1921 cgccattaac gtgtcggcct gacccctgat gtccatcgat aaagaagcgc ttttgcaggg
     1981 caaccggcgt gcccttgcaa aagccatcac gctgacggaa agcaagctgg acagccaccg
     2041 tcaggaggcc caggagttgc tggaatcgct gttgccccac accggcaaca gcatccgcat
     2101 cggcattacc ggcattcccg gcgtgggcaa gtccaccttt atcgaagcct ttggcctcca
     2161 tctcatccgg gcgggcaaga aggtcgctgt cctggcagta gaccccagtt cccccatcac
     2221 cggcggcagc atcctcggcg acaagacccg catggaagtg ctgtcccagc aggaaaatgc
     2281 cttcatacgc ccatccccca gtggcggcgc tctgggtgga gtggcgcaga aaacccgtga
     2341 aagcatgctg ctgtgcgaag ccgcaggcta tgacgtgatc ctggtggaaa ccgtgggtgt
     2401 cggtcagtcg gagtacgaag tggccagcat ggtggacttc ttcctggtcc tgatgctgcc
     2461 caatgcgggc gatgaactgc agggcatcaa gaaaggcata ctggaactgg cggatgccat
     2521 cgtgatcaac aaggcggacg ggtccgcccg cgccatggcc gatcaggccc gccaccacta
     2581 caacaatgcc ctttccattt tgcaccacca ccatttctgg caaccccggg tgctgctctg
     2641 ttctgccatg gaaaacaaga acatagatgt aatctgggag atgatcctcg agtaccgcca
     2701 gcgctcacag gaggcaggcg tcttcctcac caaacgggcc cgccagaacc aggaatggat
     2761 gtggcgactg gtgcacgaac tgatcaatcg cagcctgagt cacgatccac acgccaaggc
     2821 agtttgtgac gccatgcaaa ataaggtaaa tgccggtcag acaacgcctt acattgctgc
     2881 ccacaggatt gtagaatcct tgcgacaaat ctgattttca gcctataagt aactggttgg
     2941 tatgtccgtt gcttcaccca gatacaatca gataactttt gagtaatccg gattcacaca
     3001 aactgtgact tttgtgcatt gttccctggc taaagttcag ccagaataaa ggaataccgg
     3061 tcaatgacgg ggcatggtgg gatccgcaat gggaaccaag atgatgagtg atactgaaca
     3121 acgcggcggc aacgtttcca gtgtcgcagc cacgctcccg tcgctggtga aacaggtgaa
     3181 ggacaacacc ttcgctatcc tgtccaataa tttcagccac ttcttcagca gctgcgacga
     3241 tctgtttttc gatctcgcca gcaaggccgg ttccaacaag gaacagaatc tctatttcga
     3301 ctcgatgcgc gaagtgcgca tgaaaaagcc gcaggtctgg tccctgttca aggacggcta
     3361 cgaggaaaac ttccgcaatc tcaccaagag ccgtctttcc ggcaagaaaa aatcggcgcc
     3421 ggaaccgctc aatctggaca cgatgcagct ggtggacaag gaagacatgg aacaggacgt
     3481 agccatcacc ggcatcgtca acaaggcccg taccgaaaac caggagcagc tgtatcagct
     3541 gaactgccgc ttcgattacc tgttgccgga cgtgaaagtc aacgaaggca acaatcctct
     3601 cgatcccgcc cagatctgca atgcggtatc caaggcgctg cgtgtgctgg atctggatat
     3661 ccgctgcaag gtgatcctgc tcaagcatct ggatcgcacg gtgatcgttg aactgcgcaa
     3721 catttactcc ctcgccaatg aattgatgat caatgccggc gtt
//
LOCUS       DASUEG010000969         6196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_831310, whole genome shotgun sequence.
ACCESSION   DASUEG010000969 DASUEG010000000
VERSION     DASUEG010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831310"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX11_18215"
     CDS             <1..386
                     /locus_tag="VFX11_18215"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX4940614.1"
                     /translation="QAGMIWSDQFNQVPVSVRFYAGGDQSIRGYDYNSLSPLDAQGNL
                     VGGSRLLVGGADYLYRFRPNWLAALFMDHGGALDETNEPLFSGAGFGVRWLSPVGSLS
                     LDLAQALNGENEGKYRLHFTMGTML"
     gene            383..3931
                     /locus_tag="VFX11_18220"
     CDS             383..3931
                     /locus_tag="VFX11_18220"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX4940615.1"
                     /translation="MRTGLKLLRHLLLWLGLPLLLLAVLVVLLLFTRPGAQLAADVAM
                     GAVPQLRIEDLDGSIAHGLRASRVVWRDGSGVQVELQGLATVWDPACLLRTTFCVESL
                     VAGTLRIEVPPGVDEPAADDDTGFPPLPLPLLVLPWHLQLLQLYLAEIRIVLPERTLL
                     IGPVSLAARWSGSRVTVEQLHAAMADPWLGRADARLSGTLDMAAEWPLDLALGGSYTP
                     PLQDWQEQQVQLTGHGNLRHLQLDGTLNARLRLPGLEPLALAADVVTGSLDSRATLHR
                     LEGEYRGAPLKVQGGLAFSHVGDLTLDAILAQWGGNQIQLTGQLRDQWNVQGSLALAQ
                     PDLLAPDARGRLDGRLQVTGPRADPRVSVTGTSAELSLPALKLRQLALDASINPVSLN
                     RLQAILTAKGLDTDGQSLRDLDLRLSGDAASHELTARASLDQQGLDTRIQGSLNPDSF
                     DWTGTVSRLQVQLDRDWNLRLQQPAAVRWQQAGQRLRVARSCLVDERSRACFSLDQQI
                     AAQKGDASLDLESFDLTRLQPWLQDQLRLDGQLNSSLRLSGAWLDPSVQGDLSLSSLN
                     VRPAKGEAQGIRNATVTVRFNGRQASVDTGFDLPPELHAQSADPWQIGWGKGRVRLQR
                     GCWQVSRLQGADRVGDLCLTAEHSTRQGLKGNLDVNVDLAQALQPWLPDGFAVQGNLT
                     AAADLSLRGRDLRANLDVRSQGGNLLLDSGNGTAPVTLPYRDLHWQAGLAQEQLDTRL
                     ELLSDRLGTGQVQARIAVMQPDPAIDLTGSLEALRLDVLRPLFPRLDTFAGQISAEAR
                     VTGTLGAPRIHGEARLQDMKVQSPSLPQGIDDLVARLAFSGDRAELTGQLASGDGGAD
                     LGGQLALTDAGWRGEFNLHGRSLSLRRSEDFTVQYQPDLTLQIEPDRLHLIGTLVLQK
                     GFVWIKELPEGSVSTSDDVVFVDETGAPETTRSSHAVTADVMVSVQDKLRLRGFGGDV
                     RLSGQVRVRQDATGVATGRGTIDIVEGSYTGFGQQLKIRKGQIIFSGPLDQPYISLEA
                     IREVDTVVAGLRVSGPASEPIATLFSEPAMADSEVLYYIVTGKEPGTGTKEDNTLVQN
                     TLLSMSLMGDRPRMRNLASKVGIEDLQMGTAGTGESTEVLLSGYLSPRTYLQYGISLF
                     EPVNTLTVRYELRDNLFLEAVSGLASALDLLYSFEF"
     gene            4026..4595
                     /locus_tag="VFX11_18225"
     CDS             4026..4595
                     /locus_tag="VFX11_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940616.1"
                     /translation="MTRRNRTDSHPDKPRKGRGRKPSTEDERKDRVIQTRVPKDLEST
                     LKDAAERKRMTVSHLIRNVLEDTFRLVDGIVADSSALVENVVRDARKLAATAQGEARP
                     LDKAAEKAGNDSTPPAEESQEAAMATADPLGAVDAWQDVIVNRPSQCASCGVELVRGQ
                     RAFRGLVSQTASVGQSPALWLCPACIEKL"
     gene            4690..>6196
                     /locus_tag="VFX11_18230"
     CDS             4690..>6196
                     /locus_tag="VFX11_18230"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4940617.1"
                     /translation="MDAVGNTTHNPRRDSGMKAWLAVMEASLAAVEQTVWQGRELAEQ
                     ALAATRAIAKGADAAREEYQALAREAAIWPQRLKRISRTGWMLTKVTGSYRLWGTRSA
                     FISRRRLPAALEKLHRSNAIRFRETSLEQGGAFLKVGQLLSARADVLPKAWVDELAVL
                     QDQARVECFSAIRHQIETELGDTLENLFLEFNPEPIAAASIGQVHKAVLKDGRPVAVK
                     VQRPGLEEIINLDMSLLKLFLASIENLLPPTDLATITTEIERTIREELDYRAEAKWMQ
                     RLGDFLRDVPGVIVPRPVQALSGKRVLISEFIEGRKLTSELDARQQAGDDKGLSDLLG
                     RLLDLYLRQVLQAGCFQADPHPGNILVTADDKLVLLDFGCTMELSEQFRSGYANVLGA
                     AMMGEKESVAASLQALGFKTRSGKPDTLLAFADALLSTLRNTATAVGGDNPAWPSADD
                     ILQRGKALFSMADNDPVDKLPAEFIMLARVFTTLGGLFIHYKPKMDVTKYLL"
BASE COUNT         1093 a         1990 c         2060 g         1053 t
ORIGIN      
        1 gtcaggccgg catgatctgg agcgaccagt tcaaccaggt accggtgagc gtgcgcttct
       61 acgccggtgg tgaccagagc attcgtggtt atgactacaa cagcctgtcg ccgctggatg
      121 cgcagggcaa tctggtgggc ggcagtcgcc tgctggtggg tggcgccgac tatctgtacc
      181 ggttccggcc caactggctg gcggccctgt tcatggacca cggtggcgcg ctggatgaaa
      241 ccaacgaacc ccttttcagc ggtgccggat tcggtgtgcg ctggctgtcg ccggtgggca
      301 gcctgagcct ggatctggcg caggcgctga acggggaaaa cgagggcaaa taccgcctgc
      361 atttcaccat ggggaccatg ctgtgaggac cgggctgaaa ctgctgcgac atctgctgct
      421 gtggctgggg ctgccgctcc tgctgctggc agtgctggtg gtactgctgc tgttcacccg
      481 cccgggggcg cagctcgcag cggatgtggc gatgggcgcg gtgccgcaac tgcgtatcga
      541 ggatctggat ggctccatcg cccatggcct gcgcgccagc cgggtggtgt ggcgcgatgg
      601 cagcggcgtg caggtcgaac tgcagggtct ggccacggtg tgggacccgg cctgtctgct
      661 gcgcaccacg ttctgcgtgg aatccctcgt ggcgggcacg ctgcgcatcg aggttccgcc
      721 gggcgtcgac gagcctgctg ccgatgatga caccggcttc cctcccctgc ctctgcccct
      781 gctggtcttg ccctggcacc tgcaactgct acagctgtat ctggcggaaa tccgcatcgt
      841 gctgccggag cggacgctgc tgatcggccc cgtgtcgctg gcagcacgct ggtccggcag
      901 ccgggtgacg gtggaacagc tgcacgctgc catggcggac ccctggctgg gtcgtgccga
      961 cgcccgttta tccggcaccc tggacatggc ggcggaatgg ccgctggatc tggccctggg
     1021 gggaagctac accccgccgc tgcaggactg gcaggaacag caggtgcaac tgacgggcca
     1081 tggcaatctg cgccacctgc agctggacgg aaccctgaac gccaggctgc ggctgccggg
     1141 gctggagccg ctggccctgg cagcagatgt ggtcaccggc agcctcgaca gtcgcgccac
     1201 cctgcaccgg ctggaggggg aatatcgcgg tgcgccgctg aaggtgcagg gtggactggc
     1261 cttcagtcac gttggcgacc tgaccctgga cgccattctg gcgcagtggg gtggcaacca
     1321 gatccagctg acgggtcagc tgcgggatca gtggaatgtg cagggcagcc ttgccctggc
     1381 gcaaccggat ctgctggcgc cggacgcccg cggccggctg gacggcaggt tgcaggtgac
     1441 gggtccccgc gccgatcccc gggtcagcgt caccggcacc agcgccgaac tgtcgctgcc
     1501 ggcgctgaag ctgcggcaac tggcgctgga tgccagcatc aatccggtca gtctgaaccg
     1561 gttgcaggcc atcctcactg ccaaggggct ggacaccgac ggccagtccc tgcgggatct
     1621 ggacctgcgc ctgagcggtg acgctgccag ccatgaactg accgcccgtg cctctctgga
     1681 ccagcagggg ctcgacaccc ggattcaggg cagcctcaac cccgacagtt tcgactggac
     1741 cggcaccgtg tcccggctgc aggtgcaact ggatcgcgac tggaacctgc gtctgcagca
     1801 gccggccgct gtgcgctggc aacaggcagg gcagcgcctg cgggtggcgc gcagctgtct
     1861 ggtggatgag cgcagccgcg cctgcttcag cctggaccag cagatcgccg cgcaaaaagg
     1921 cgatgcctcc ctcgacctgg agtcgttcga tctgacccgg ctgcaaccct ggctgcagga
     1981 ccagctgcga ctggacggcc agctcaattc cagcctgcgc ctgagcggcg cgtggcttga
     2041 cccatccgtg cagggcgacc tgtcactttc cagtctgaat gtgcgtcccg ccaagggtga
     2101 agcgcagggc atccgcaatg ccacggtgac ggtgcgcttc aacggccgcc aggccagtgt
     2161 ggacaccggt ttcgatcttc cccctgaatt gcatgcccag tcagccgacc cctggcagat
     2221 cggctggggc aaggggaggg tccggctgca gcgcggctgc tggcaggtct cccggctgca
     2281 gggtgcggat cgggtaggcg atctgtgcct gactgccgag cactccaccc gccagggtct
     2341 taagggcaac ctggatgtca atgtggatct ggcccaggcg ctgcagccct ggctgccgga
     2401 cggattcgcc gtgcagggca atctcaccgc cgccgccgat ctcagcctgc ggggccgtga
     2461 cctgcgcgcc aacctggatg tgcgctcgca gggaggcaat ctgctgctgg acagtggcaa
     2521 cggtaccgcc ccggtaacgc tgccctaccg ggacctgcac tggcaggccg gtctggcaca
     2581 ggagcagctg gacacccgcc tggaactgct ttcggacagg ctcggcactg gtcaggtcca
     2641 ggcccggatt gccgtgatgc agccggaccc cgccatcgac ctgaccggca gtctggaggc
     2701 ccttcgcctg gatgtcctgc gccccctgtt cccccgcctg gatacgttcg ccgggcagat
     2761 cagcgcggag gccagggtta ccggcaccct gggggcaccg cgcattcatg gcgaagcccg
     2821 gctgcaggat atgaaagtgc aaagcccgag ccttccgcag ggcatcgatg atctggttgc
     2881 ccgcctggcg ttcagcggcg accgcgccga actgacgggt cagctggcca gcggcgacgg
     2941 cggcgcggat ctcggcggac agctggccct taccgatgcc ggctggcgcg gcgagttcaa
     3001 cctgcacggg cgcagcctgt ccctgcgccg gtccgaggat ttcaccgtcc aataccagcc
     3061 ggacctcacc ttgcagatcg agccggaccg cctgcacctg atcggcaccc tggtgttgca
     3121 gaagggcttc gtctggatca aggaactgcc ggaaggctcc gtgagcacct cggacgatgt
     3181 ggtgttcgtg gatgaaacgg gcgcgccgga aacgacccgc tccagccacg ccgtgacggc
     3241 ggatgtcatg gtgagcgtgc aggacaagct gcggttgagg ggcttcggcg gtgacgtccg
     3301 tctgagcgga caggtgcggg tgcgccagga cgccaccggc gtggctaccg ggcgcggcac
     3361 catcgacatc gtcgagggca gctacaccgg cttcggccag cagctcaaga tccgcaaggg
     3421 ccagatcatc ttcagcggcc ccctcgacca gccttatatc agcctggaag ccatccgcga
     3481 ggtcgacacc gtggtagccg gcctgcgggt cagcggcccc gccagcgaac cgatcgccac
     3541 cctgttttcg gaacccgcca tggccgacag cgaagtgctt tattacatcg tcaccggcaa
     3601 ggaaccgggc accggcacca aggaggacaa caccctggtg cagaacaccc tgctcagtat
     3661 gagtctgatg ggcgaccggc cgcgcatgcg caacctggcc agcaaggtgg gcatcgaaga
     3721 cctgcagatg ggtaccgccg gcaccggcga aagcaccgaa gtgctgttgt cgggctacct
     3781 gtcgccgcgc acctacctgc aatacggcat cagcctgttc gagccggtga acaccctcac
     3841 cgtgcgttac gaattgcgtg acaacctgtt cctggaagcg gtgagtggcc tggccagcgc
     3901 cctcgacctg ctgtacagct ttgaattctg aagcatccat ccctgatgac aggcggctgg
     3961 cccgggttga cacctgaccg gccttgccat attttgtatt acaacaattc tggacaggac
     4021 tatccgtgac ccgccgcaat cgcaccgaca gccacccgga caagccccgc aaaggacgcg
     4081 gccgcaaacc gtccaccgag gacgagcgca aggaccgcgt gatccagacc cgggtgccga
     4141 aagacctgga aagcaccctc aaggatgcgg cggaacgcaa gcgcatgacc gtctcccacc
     4201 tgatccgcaa cgtactggaa gacaccttcc gtctggtgga cggcatcgtc gccgattcca
     4261 gtgcgctggt ggaaaatgtg gtccgcgacg cccgtaagct ggcggcaacc gcccagggtg
     4321 aggccaggcc gctggacaag gcggcggaaa aagccgggaa tgactccacc ccacctgcgg
     4381 aagaatcgca ggaagccgca atggccaccg ccgacccgct gggtgccgtc gatgcctggc
     4441 aggacgtgat cgtcaaccgc ccgagccagt gtgcgtcctg tggtgtggaa ctggtgcgcg
     4501 gtcagcgcgc ctttcgcggc cttgtcagcc agaccgccag cgttggacag agcccggcac
     4561 tctggctctg ccccgcctgt atcgaaaaac tgtaaccgct ttacatctcc ggagcccaac
     4621 ccgctccggt ttattacaaa ctgatttgta ctacaaaaaa tgacatgaaa cctgacccgg
     4681 gaggtcatca tggacgcagt cggaaacacc acgcacaacc cccgccgcga cagtggcatg
     4741 aaagcctggc tggccgtcat ggaagcctcc ctcgccgcgg tcgagcagac cgtgtggcag
     4801 gggcgcgaac tggcagagca ggcgttggcc gccacccgcg cgattgccaa gggcgccgac
     4861 gccgcgcggg aggaatacca ggctctggca cgggaagccg ccatctggcc gcagcgcctg
     4921 aaacgtattt ccagaactgg ctggatgctt accaaagtca ccggcagtta ccgcttgtgg
     4981 ggcacgcgat ccgctttcat ctcccgccgt cgcctgcctg ccgcactgga aaaactgcac
     5041 cgcagcaatg ccatccgttt ccgcgaaacg tcgctggagc agggcggtgc tttcctgaaa
     5101 gtggggcaac tgttgtcggc ccgcgcggat gttctgccca aagcctgggt ggacgaactg
     5161 gccgtgctgc aggaccaggc ccgggtggaa tgcttcagcg ccatccgcca ccagatcgaa
     5221 accgagctgg gcgacacgct ggaaaacctg tttcttgaat tcaatcccga gcctatcgcc
     5281 gccgccagca ttggtcaggt gcacaaggcg gttctgaagg acgggcgccc ggtggcggtg
     5341 aaagtgcagc gccccggcct ggaagaaatc atcaatctgg atatgtcgct gctgaagctg
     5401 ttcctggcca gcatcgaaaa cctgctgcca cccacggacc tggccaccat caccaccgaa
     5461 atcgagcgca ccattcgcga agagctggat taccgggcgg aagcgaaatg gatgcagcgg
     5521 ctgggggatt ttctgcgcga tgtgcctggc gtgatcgtgc cgcgtccggt gcaggcactg
     5581 agcggcaagc gggtgctgat cagtgaattc attgagggcc gtaaactcac ttccgaactg
     5641 gacgcgcgcc agcaggccgg tgacgacaag gggctgtccg atctgctcgg ccggctgctg
     5701 gatctgtatc tgcgccaggt gctgcaggcc ggttgtttcc aggccgatcc gcacccgggc
     5761 aatattctgg tcactgccga cgacaagctg gtgttgctgg attttggctg caccatggag
     5821 ttgtcggagc agtttcgcag cggctacgcc aatgtgctgg gcgcggcgat gatgggcgag
     5881 aaggagagtg tggccgcgtc gctgcaagcc ctgggtttca agacccgcag tggcaagccg
     5941 gacacgctgc tggcgtttgc cgatgcgttg ctttcaacgc tgcgcaacac ggccacggcg
     6001 gttggtggcg ataatcctgc ctggcccagc gcggacgata ttttgcagcg cggcaaagcc
     6061 ttgttctcga tggcggataa tgatccggtg gacaagctgc cagcggaatt catcatgctg
     6121 gcgcgggtgt tcacgacgct gggcgggttg ttcatccatt acaagccgaa gatggatgtg
     6181 acgaagtatt tgctgc
//
LOCUS       DASUEG010000970         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_765248, whole genome shotgun sequence.
ACCESSION   DASUEG010000970 DASUEG010000000
VERSION     DASUEG010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765248"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VFX11_18235"
     CDS             complement(<1..1554)
                     /locus_tag="VFX11_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001998041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX4940618.1"
                     /translation="MSGRFSVGIDLGTTNSVVAYVDLQSGEDNPALSVLPIPQLTQPG
                     VVENRNQLPSFLYQPHESEVSAQELVLPWGSSESSLVGVIARHLGSKTPLRLVSSAKS
                     WLCHGGVDRHAAFLPLNTPEDVAKVSPLQASIAYLNHMKAAWNHQYPGFALEQQDVTL
                     TVPASFDPAARDLTVEAARAIGLHNLTLLEEPQAALYSWIKASGEQWRKTVKVGDVIL
                     VVDVGGGTTDLSLIAVTEEDGNLQLNRIAVGDHILLGGDNMDLALAYRLKAKLDAEGK
                     QLQPWQIQALTHGCRDAKEILLGDSGETAVPIVVPSRGSKLLGATLRTELTREEVEQT
                     LVDGFFPQVAIHEHPRQALRGALSQLGLPYAQDAGVTRHLAAFLAKQANATADLFGHA
                     GANFIKPTAVLLNGGVLKAPALAQRLMSVINGWLQQAGAEAARLLEGADLDLAVARGA
                     AYYGFVRKGHGVRIRGGIANAYYVGIESAMPAVPGMAPPIQALCVAPFGMEEGSETSP
                     SFAEFGLIVG"
     gene            complement(1551..2126)
                     /locus_tag="VFX11_18240"
     CDS             complement(1551..2126)
                     /locus_tag="VFX11_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HEX4940619.1"
                     /translation="MTFDPTALPATLDALHLVLGLSSVVFLALLFAKSKPAPAVPESR
                     AVALPVEAPKPAPQPVPALKTHSPDAALQLLSLLQQEARFVDFLHEDLKGFSDAEIGA
                     VARVVHEGGKKTLNQYFKLEPVRSETEESRVTLPAGFNAAEVRVTGNVVGQPPFTGTL
                     VHRGWKAVEANLPKLADGHDARIIAQAEVEL"
     gene            2420..3304
                     /gene="htpX"
                     /locus_tag="VFX11_18245"
     CDS             2420..3304
                     /gene="htpX"
                     /locus_tag="VFX11_18245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX4940620.1"
                     /translation="MMRIALFLLTNLAVLAVAGITLSVLGVPGYLQQGGASINLQALL
                     IYCAVFGFAGSFISLFLSKPMAKWSSGTQVITQPRNQAEHWLLETVRELSTKAGIGMP
                     EVGVFPSPVSNAFATGWNKNSALVAVSEGLLHRFDREEVRAVLAHEIGHVANGDMVTL
                     SLIQGIVNTFVMFFARIASFAVDQFFRRDDGEGMGGIARMAVTFVFEMIFGILASIIV
                     MWFSRQREYRADAAGADLAGRGAMIRALQRLKAESEAPNDLPQTMTAFGIRSHDMMAL
                     FASHPPLDQRIAALQGGR"
BASE COUNT          680 a         1096 c          926 g          643 t
ORIGIN      
        1 acccacgatc aagccaaact cggcaaagga cggcgatgtt tcactgccct cctccatgcc
       61 gaacggcgcc acgcacaagg cctgaatcgg cggcgccatg ccaggcacgg caggcatcgc
      121 actttcaatt cccacgtaat aggcgttggc gataccacca cggatacgca cgccatgacc
      181 cttgcgcaca aatccgtaat aggccgcacc ccgcgccacc gccagatcca gatcggcgcc
      241 ttccagcaaa cgcgccgctt ccgcgcccgc ctgttgcagc cagccgttga tcacactcat
      301 caaacgctgc gccagcgccg gcgctttcag cacgccacca ttcaacagca ctgccgtagg
      361 cttgatgaaa ttcgctccgg cgtggccgaa cagatccgcc gtcgcattgg cctgcttcgc
      421 cagaaacgcc gccagatgac gggtaacacc tgcatcctgc gcatagggca gacccagctg
      481 cgacaacgca cctcgcaacg cctgcctggg atgttcatga atcgcaacct gcgggaagaa
      541 accgtccacc aacgtctgtt ccacttcttc gcgggtgagt tcggtgcgca aggtggcacc
      601 caataatttg gaaccgcggc tcggcaccac gatgggaaca gccgtttcgc cactgtcgcc
      661 cagcagaatt tccttggcat cgcgacagcc gtgggtcagg gcctggatct gccaaggttg
      721 caactgcttg ccttccgcgt ccagcttggc tttcagacga tacgccagcg ccagatccat
      781 gttgtcgccg cccagcagga tgtgatcacc caccgcaatg cgattcagtt gcaggttgcc
      841 gtcttcttcc gtcaccgcga tcagcgacag atccgtggtg ccaccaccaa catccaccac
      901 cagaatcaca tcgccgactt ttacggtttt gcgccactgc tcgccactgg ctttgatcca
      961 gctgtacagc gccgcttgcg gttcttccag cagggtcaga ttgtgcagac caatggcgcg
     1021 cgccgcctcc acggtgagat cgcgcgcagc cggatcgaac gacgccggca cggtcagcgt
     1081 cacatcctgc tgctccagcg caaatcccgg atactggtga ttccacgccg ctttcatatg
     1141 attcagatag gcaatcgacg cctgcaacgg cgacactttc gccacatctt ccggcgtatt
     1201 cagcggcaga aatgctgcat gccgatccac accaccgtga cacaaccagc ttttcgccga
     1261 cgacaccagc cgcaacggcg tcttgctgcc cagatgccgg gcaatcacgc ccaccagcga
     1321 tgattcgctg ctgccccagg gcagcaccag ttcctgcgcc gacacttccg attcgtgcgg
     1381 ctggtagaga aacgacggca gctggttgcg gttttccacc acgcccggct gggtcagctg
     1441 cggaatcggc aacacgctca gcgccggatt gtcttcacca gattgcagat ccacatacgc
     1501 caccacgctg ttggtggtgc ccagatcgat gccaacggaa aaacgaccac tcacagttca
     1561 acctcggctt gcgcaatgat gcgggcgtcg tgaccgtccg ccagtttcgg cagattggct
     1621 tccaccgctt tccagccccg gtgcaccagc gtgccggtga agggcggctg gcccaccaca
     1681 ttgccggtca cccgcacttc ggcagcgtta aaaccggccg gcagtgtcac gcgggattct
     1741 tccgtttcac tgcgcaccgg ttccagtttg aaatactgat tcagggtctt cttgccaccc
     1801 tcatgcacca cccgcgccac cgcgccgatt tccgcgtcgg aaaaaccttt cagatcctcg
     1861 tgcaggaaat ccacgaagcg ggcttcctgc tgcagcaggc tgaggagttg cagggcagca
     1921 tccggcgaat gggttttgag ggcaggtaca ggctggggtg caggtttcgg agcttctacc
     1981 ggcagtgcca ccgcccggct ttccggtacg gccggcgctg gtttcgattt cgcgaacagc
     2041 agcgccagaa acacaacaga ggaaaggcca agtaccagat gcagggcgtc cagggtggca
     2101 ggcagggcgg tcggatcaaa cgtcatggcg gattctcgac ttgtactgga aaggaggggg
     2161 tggaaacccg tacaaatacc ggcccgactg gggccggcgc acaaagtgcc gcgcagttta
     2221 ccatcttgcc gaccgcaata aagaccacag aaagctggtc ttttatgttt catgaaacca
     2281 tgacatgata tgtcatcgaa acttatggtt tccgggttgt ccaacgtcag gcggctgttg
     2341 caattcaagc acttgaaatg aaccgcgcag cctccataac aggacagtgt gattcctaac
     2401 aagaggtcaa gaattcacca tgatgcgtat agcccttttt ctcttaacca acctggccgt
     2461 tctggcggtt gccggcatca ctttgagcgt gctgggcgtg cccggctacc tgcaacaggg
     2521 cggcgcctcc atcaacctgc aggcgttgct gatctactgt gccgtgttcg gcttcgccgg
     2581 ctccttcatc tccctgttcc tgtccaagcc catggccaag tggagctcgg gcacccaggt
     2641 cattacccag ccccgcaacc aggcggaaca ctggctgctg gaaaccgtgc gcgaactgtc
     2701 taccaaggca ggtatcggca tgccggaagt gggcgtgttc ccgtccccgg tatccaacgc
     2761 tttcgccacc ggctggaaca agaacagtgc actggtagcc gtgagcgaag gcctgctgca
     2821 ccgctttgac cgcgaagaag tccgcgcggt actggcccat gagatcgggc acgtggccaa
     2881 cggcgacatg gtgaccctgt cgctgatcca gggcatcgtc aacaccttcg tcatgttctt
     2941 cgcccgcata gcgtccttcg cggtggatca attcttccgc cgtgacgatg gcgaaggcat
     3001 gggtggcatt gcccgcatgg ccgttacctt cgtgttcgag atgatcttcg gcatcctggc
     3061 cagcatcatc gtgatgtggt tctcgcgcca gcgcgaatac cgggccgacg ccgccggcgc
     3121 cgatctggcc ggtcgtggtg ccatgatccg tgcgctgcaa cgcctgaagg cggaaagcga
     3181 agcgcccaac gatctgccgc agaccatgac tgcctttggt atccgcagcc acgacatgat
     3241 ggcactgttt gcctcgcatc cgccgctgga ccaacgcata gccgcgctgc aaggcggtcg
     3301 ctgagcgctg aaatctgaac gcaagcactc atcagcagca aacaa
//
LOCUS       DASUEG010000971         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1948211, whole genome shotgun sequence.
ACCESSION   DASUEG010000971 DASUEG010000000
VERSION     DASUEG010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948211"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFX11_18250"
     CDS             <1..961
                     /locus_tag="VFX11_18250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX4940621.1"
                     /translation="KGLIAFAEGDWSNAERLMAKGATGNEVALINYLTAAQAAHEQGQ
                     EDRRDDYLRLAHQSTKGVETAIALTKARLQFRSEQWEESLATLMVLKQNEKLPSYPTV
                     IKMLAQVYIKLEDWDSLLQLLPELHKRKVVEPDEHNELSRRCHVGKLKQSLRGSEEDR
                     LHNLRQAWEQVPRKLHHDPALLQTYVEALIQLNAGDEAERLITDALRKDWDDNLARLY
                     GTVRGGDVAKQLQWAENWLRERPNNAMLLLTLGRLSLQLQQWEQARQYFEASLRARKS
                     AEAHGELGRLLSHLGEHQASNDNFQQGLAMISQRLPDLPMPER"
     gene            1095..1472
                     /locus_tag="VFX11_18255"
     CDS             1095..1472
                     /locus_tag="VFX11_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX4940622.1"
                     /translation="MNDINPAPDQQRELSPDERNWGMFCHLAGFAGIIIPFGSIIGPL
                     VIWLIKRDEMPFVNYNGKEALNFQITYMVAFLVSVVLMSVVIGFLMMAVVGIAWLVFT
                     ILAISHSSKGEYYRFPYIFRVIN"
     gene            complement(1522..1965)
                     /locus_tag="VFX11_18260"
     CDS             complement(1522..1965)
                     /locus_tag="VFX11_18260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4940623.1"
                     /translation="MSTSHSFSVNLTLLEGYVFKVDFGDMGDILTDEAPPLGNGEGPN
                     PSRLVAAAVGNCLCASLLFALRKAKEQPGGVSAVVTGDLERQDGRWRIGRLDVKLQVE
                     NAQTLQQLPAVLEKFEEYCIVTQSIRQGIPVNVEVVDQDGKVLKA"
     gene            2162..>2579
                     /locus_tag="VFX11_18265"
     CDS             2162..>2579
                     /locus_tag="VFX11_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940624.1"
                     /translation="MGKGKQTYVFEHQLKTLVNIRERIDLLVQKHGELKGGVPGVLFR
                     TAVDPHSPTLYQGDALGKRRIVQGVREGDYLFSESDPSWIQPSDEHGLSFSSSFNQVK
                     FTLDLLGKFQKKGTKINVAYWILESSIHIPEDMAFVQ"
BASE COUNT          570 a          751 c          740 g          518 t
ORIGIN      
        1 caagggcctg atcgcgttcg cggaaggcga ctggtccaac gctgaacgcc tgatggccaa
       61 gggcgccacc ggcaatgaag tggcgctgat caactacctc accgctgccc aggccgccca
      121 tgaacagggc caggaagacc gccgcgacga ttacctgcgg ctggcgcacc agtccaccaa
      181 gggtgttgaa accgcgattg cgctcaccaa agcccgcctg caattccgca gcgagcagtg
      241 ggaggaatcc ctggccaccc tgatggtgct caagcaaaac gaaaaactgc ccagctaccc
      301 caccgtcatc aaaatgctgg cgcaggtgta catcaaactg gaagactggg acagcctgct
      361 gcagctgctg ccggagctgc acaagcgcaa ggtggtggaa ccggatgaac acaacgaact
      421 ctcacgccgc tgccacgttg gcaaactgaa acagtccctg cgtggcagcg aggaagatcg
      481 cctgcacaac ctgcggcagg cctgggagca ggtgccgcgc aaactgcacc acgatccggc
      541 cttgctgcag acctatgtcg aagcgctgat ccagctgaat gccggcgacg aagccgagcg
      601 tctgattacc gatgcgctgc gcaaggactg ggacgacaac ctggcccgcc tgtacggcac
      661 cgtgcgtggc ggcgacgtgg ccaaacagct gcaatgggcg gagaactggt tgcgggagcg
      721 ccccaacaac gccatgctgc tgctcaccct gggccgcctg agcctgcaac tgcaacagtg
      781 ggaacaggca cgccagtatt tcgaagccag cctgcgcgca cgcaaatccg ccgaggcgca
      841 cggtgagctg gggcgtctgc tcagtcacct gggcgaacac caggccagca acgacaattt
      901 ccagcagggg ttggcgatga tctcgcagcg gttgccggat ttgccgatgc cggagcggta
      961 attgcgtacc accgccagcg actgtttgca cagaattggc ggtcatactg ctgctgatgg
     1021 attgcttgca attgggcggg aatttgcaga atctatcccc gattcactca cggaacgaaa
     1081 ccaaaggggg caccatgaac gacatcaatc cagcaccgga ccagcagcgt gagctgtcgc
     1141 cggacgaacg caactggggc atgttctgtc acctggcggg gttcgccggg atcatcattc
     1201 ccttcggcag catcatcggc ccgctggtga tctggctgat caagcgggat gaaatgcctt
     1261 tcgtgaacta caacggcaag gaagcgctga atttccagat cacctatatg gtggccttcc
     1321 tcgtaagtgt ggtgctgatg tccgttgtca tcggattcct gatgatggcg gtggtgggga
     1381 ttgcctggct ggtgttcacc atcctcgcca tctcgcacag cagcaagggc gagtactacc
     1441 gcttcccgta tatcttccgc gtcatcaact gatcttttga gcgacctgct gcggcgcacc
     1501 gccgcaacag gtcgctcatt gtcacgcctt caatactttt ccgtcctgat ccaccacttc
     1561 cacattcacc ggaatcccct ggcggatact ctgggtgaca atgcagtatt cctcgaattt
     1621 ctccaacact gccggcagct gctgcagcgt ctgtgcgttt tccacctgga gtttcacgtc
     1681 cagccggccg atgcgccagc gcccatcctg ccgctccaga tcacccgtta ccacggcgct
     1741 cacaccgccg ggttgctctt ttgccttgcg caaggcaaac agcaggctgg cgcacaggca
     1801 gtttcctact gccgctgcta ccaggcgcga cggattgggc ccttcgccat tgcccaaggg
     1861 cggcgcctcg tccgtgagga tgtcgcccat atcgccgaaa tcgactttga aaacgtagcc
     1921 ttcgagaagg gtgaggttga cggagaagga gtgtgaggtg ctcatgacag cgtgccctgt
     1981 ttcggtctgg aatactttta ctataaccaa gcaacggtta catgcgaatg agatgggtca
     2041 aaatgtctgg ttgaatggac gcagtgaaaa tgtacggatt tgatgtcgta ataattcaac
     2101 agacgtcgtc gttttgctgg cggacactgt cgactgtatc caacctcata cggcaattac
     2161 catgggcaaa gggaagcaaa cgtacgtttt cgaacatcag ctcaaaacgc tggtgaatat
     2221 ccgggaacga attgatctac tggtacagaa acatggtgag ctgaaaggcg gtgtccctgg
     2281 cgtcctgttt cgtacagcgg ttgatccaca ttcgcccacc ctgtatcagg gtgatgcctt
     2341 gggcaagcgg cgaattgtcc agggcgtcag agaaggcgat tatctgtttt ccgagtctga
     2401 ccccagctgg atccagccca gtgacgagca cgggctgtca ttctccagca gcttcaatca
     2461 ggtcaagttc acgctggact tgctggggaa gtttcagaag aagggcacta aaatcaatgt
     2521 cgcctactgg attctggaaa gctccattca tattccagag gatatggcat ttgtgcagg
//
LOCUS       DASUEG010000972         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1848043, whole genome shotgun sequence.
ACCESSION   DASUEG010000972 DASUEG010000000
VERSION     DASUEG010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848043"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /gene="sat"
                     /locus_tag="VFX11_18270"
     CDS             complement(<1..263)
                     /gene="sat"
                     /locus_tag="VFX11_18270"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260733.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX4940625.1"
                     /translation="MSKLVKPHGSDVLKTLLLEGDALASAQAAAAKLPQVKLSSRETG
                     DLIMLGIGGFTPLDGFMGKADWQSVCDRMTLASGLFWPIPITKS"
     gene            complement(361..1119)
                     /locus_tag="VFX11_18275"
     CDS             complement(361..1119)
                     /locus_tag="VFX11_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX4940626.1"
                     /translation="MISRDALLAELNQLLQPQYFKDYAPNGLQVQGRESIQTLVTGVT
                     ATRALIERAVALKADAIFVHHGYFWRSEDPAITGMKYQRVKLLLDHGINLFAYHLPLD
                     AHPELGNNAQLGKLLGFKTEGGLQPGEPRPIGNVGVLDAPLTLAQLEALVAARLGRPP
                     LAIRGGDHPIKRVAWCTGGAQDYIEQAVALGADAYISGEISEQTVHVARECGIHYLAA
                     GHHATERGGARAVGQYLAQKFQLDHQFVDIDNPA"
     gene            1223..2428
                     /locus_tag="VFX11_18280"
     CDS             1223..2428
                     /locus_tag="VFX11_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4940627.1"
                     /translation="MASDFMKSLARFLVTPVLAGLAVAALFLLLNNREPPVQRHEIVL
                     IEQQVPARETQSTGLAPETRPQLNAAQGDPVSYADAVARAQPAVVNIFSTKIVTREYH
                     PLLDDPNFRRFFGLNEAPRRERMLSSLGSGVIVSPTGYVLTNNHVISGADEIRVALAD
                     GRETFAAVVGTDPETDLALLFIEMPDLPAIALTKSDAIRVGDVVLAIGNPFGVGQTVT
                     KGIVSALGRHNNLSVFVDFIQTDAAINPGNSGGALINAYGDLVGINTAIYSKSGGSQG
                     IGFAIPVDTAKSVMMELLQHGQVIRGWLGVEPQVLTDALIKALDLPDINGLLVKGIFK
                     NGPAHVAGVRPGDIITHLNGEAVNDPKVAMNLISGLQPGEKVSIRVIRQREPLEIVAV
                     AAARPVPGN"
     gene            2463..3782
                     /locus_tag="VFX11_18285"
     CDS             2463..3782
                     /locus_tag="VFX11_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX4940628.1"
                     /translation="MTTPASTPTLQRHIGLIALTLYGVGDILGAGIYGLIGRAAGEMG
                     HAIWLAFLVSMIAAGLTGLSYASLGSRFPRAGGAAYISLRAYRKPFISYMVGLAVLAS
                     GLTSMATAAKVFGGYLNGLFPALPPLAIALGFSLLIAGIVFRGIRESLFANGIFTLIE
                     VSGLFLIIAAGLPYLGSVNYLDAATPQNLADDISLSLALTGAVLTFYSFVGFEDLLNV
                     AEEVKEPQKTLPRALLLAVLIASLIYMTISLVAVSVLAPAALAGSQQPLVDVAAKIAP
                     WFPSSAFSLIALFAVANTALLNFVMGSRLLYGMASHGLVPRVLSRVHAGRHTPHMAII
                     AVWVIFVVLVLSGDISSLARATSVLLLCCFVLVNGALVVLKTRESWHGHFDIPVFVPV
                     LGALICLAMLSQAKAPELLTAGAILAGIVVLYFVMRPSADALEHMDD"
BASE COUNT          648 a         1235 c         1259 g          794 t
ORIGIN      
        1 gacttggtga tggggatcgg ccagaacagg ccgctggcca gggtcatcct gtcgcacacg
       61 gattgccagt cggccttgcc catgaagccg tccagcgggg tgaagccgcc gatgcccagc
      121 atgatcaggt cgccggtttc gcgggaggac agttttacct ggggcaattt ggcggcggcg
      181 gcctgggcac tggccagggc atcgccttcc agcaggagcg ttttcaacac atcgctgccg
      241 tggggcttca ctagtttcga catggcactt ctcgttccgg ttgacgggga aaatggggtg
      301 gctgcaacgc agggattctg cgcaaaaggg gctgcattat aaccgttccg gcgcctgcgg
      361 tcaggccgga ttgtcgatat ccacaaactg gtgatccagc tggaattttt gcgccagata
      421 ctggcccacg gcgcgggcac cgccccgttc ggtggcatgg tggcccgccg ccaggtagtg
      481 aatgccgcac tcccgcgcca cgtgcaccgt ctgttccgag atctcgccgc tgatgtaggc
      541 atccgccccc agcgccaccg cctgttcgat atagtcctgg gcgccgccgg tgcaccaggc
      601 cacccgcttg atggggtgat cgccaccgcg gatggccagg ggcgggcgac ccagcctggc
      661 agccaccagc gcttccagct gcgccagggt cagcggcgca tccagcacgc ccacattgcc
      721 aatcggacgc ggctcgccgg gttgcaggcc gccttcggtc ttgaacccca gcagcttgcc
      781 cagttgcgcg ttgttgccca gttcggggtg ggcgtccagc ggcaggtgat aggcgaacag
      841 gttgatgccg tggtccagca gcagcttcac ccgctggtat ttcatgccgg tgatcgcggg
      901 atcttcgctg cgccagaaat agccgtggtg aacgaagatc gcatcggcct tcaatgccac
      961 cgcacgttcg atcagcgcgc gggtggcggt gacgccggtg accagcgtct ggatcgactc
     1021 acgcccctgc acctgcaagc cgttgggcgc gtaatccttg aaatactgcg gttgcagcag
     1081 ctggttgagt tcggcgagca aggcgtcgcg gctgatcatg aatccggtgt ctccgtcagg
     1141 gcgcgtggtg cacggcgccg ttccgtgtta atttgctttg gcatctcttt ccaatcccga
     1201 ttattccaaa caccgcgacg gaatggccag cgactttatg aaatcccttg cccgctttct
     1261 ggtgacgccg gttctggccg gtctggctgt ggccgccctg tttctgctgc tgaacaaccg
     1321 cgagccgccg gtgcagcgtc acgagatcgt gctgatcgaa cagcaggtgc cggcgcggga
     1381 aacccagagc accgggctgg cgccggaaac gcgtccccaa ttgaacgccg cccagggcga
     1441 tccggtgtcc tatgccgatg ccgtggcccg cgcccagccg gcagtggtga acatcttcag
     1501 caccaagatc gtgacccgtg aataccatcc cctgctggac gaccccaatt tccgccgttt
     1561 tttcgggttg aacgaggcgc cacgacgcga acgcatgctg tccagtctgg ggtccggcgt
     1621 gatcgtcagc cccaccggct acgtgctgac gaacaaccat gtcatcagtg gcgccgatga
     1681 aatccgcgtg gccctggcgg acgggcgcga aacctttgcc gctgtggtgg gcactgatcc
     1741 ggaaaccgat ctggccctgc tgttcatcga aatgcccgac ctgcccgcga tcgccctgac
     1801 caagagcgat gcgatccgtg tgggcgacgt ggtgctggcc atcggcaatc ccttcggcgt
     1861 gggccagacg gtaacgaagg gcatcgtgag tgccttgggc cgccacaaca acctgagcgt
     1921 gttcgtcgat ttcatccaga ccgacgccgc catcaacccc ggcaattccg gcggcgcgct
     1981 gatcaatgcc tatggcgatc tggtgggaat caacaccgcg atctattcca aatccggcgg
     2041 ttcccagggc atcggctttg ccattcccgt ggacaccgcc aaaagcgtga tgatggaact
     2101 gctgcagcac ggccaggtga ttcgcggctg gctgggtgtg gagccacagg tgctcaccga
     2161 tgcgctcatc aaggccctgg acctgcccga catcaacggc ctgctggtga aaggcatttt
     2221 caagaacgga cccgcccatg tggccggcgt gcgccccggc gacatcatca cccatctgaa
     2281 tggcgaggcg gtgaatgatc ccaaggttgc catgaacctg atctccggcc tgcagccggg
     2341 ggaaaaggtc agtatccggg tgattcgtca gcgcgagcca ctggagatcg tcgccgtggc
     2401 agccgcgcgg cccgtgcccg gcaattgaga tttcacgttg cggtttcact gttgaggttc
     2461 tgatgacgac accggcttcc acccccaccc tgcagcgtca tatcggactg atcgcactga
     2521 ccctgtacgg cgtgggcgac attctggggg cgggcatcta cggcctgatc gggcgcgctg
     2581 ccggcgaaat gggtcacgcc atctggctgg cgtttctggt gagcatgatt gccgccgggc
     2641 tcaccggcct gtcctacgcc agcctggggt cgcgctttcc gcgggcgggc ggcgctgcgt
     2701 atatctcgct gcgcgcctac cgcaaaccgt tcatttccta catggtgggg ctggccgtgc
     2761 tggcatcggg gctcacctcc atggccaccg ccgcgaaggt attcggcggc tacctcaacg
     2821 gcctgttccc cgcgctgcca ccgctggcga ttgccctggg cttttcgctg ctgatcgccg
     2881 gcatcgtgtt tcgcggcatt cgcgaatccc tgttcgccaa cggcattttt accctgattg
     2941 aggtcagcgg gctgtttctg atcatcgcag ccggactgcc ctatctgggc tcggtcaatt
     3001 acctggatgc cgccacaccg cagaatctgg cagacgatat cagcctgtcg ctggcgctga
     3061 ccggagcggt gctgacgttc tattccttcg tcggtttcga ggacctgctg aacgtggcgg
     3121 aggaagtgaa ggaaccgcag aaaaccctgc cccgcgccct gctgctggcg gtgctgattg
     3181 cttcgctgat ctacatgacc atcagtctgg tggcggtatc ggtgctggca ccggccgcgc
     3241 tggccggatc gcaacagccg ctggtggacg tggccgcgaa gatcgcgccc tggttcccaa
     3301 gttcggcctt cagcctgatc gcgctgttcg cagtggcgaa tacggcgctg ctgaatttcg
     3361 tcatgggctc gcgcctgctg tacggcatgg cgtcacacgg gctggtgccg cgcgtgctgt
     3421 cccgcgtgca tgcgggccgg cacacgccgc atatggccat cattgcggta tgggtgattt
     3481 tcgtggtgct ggtgctgagc ggtgacattt catcgctggc acgagccacc agcgtactgc
     3541 tgttgtgctg tttcgtgctg gtgaacggtg cgctggtggt attgaaaacg cgggaatcct
     3601 ggcacggcca tttcgacatt ccggttttcg tgccggtact gggcgcgctg atctgcctgg
     3661 cgatgctgag ccaggcaaag gcgccggaac tgctcaccgc cggagcaatt ctggcgggta
     3721 ttgtggtgct gtatttcgtg atgcggccgt cggcggatgc gctggagcat atggatgatt
     3781 aggctgcagt accgacaacc cgtccagcct cgattgttgc cgccatgccg gagcatccac
     3841 cagaaacagc ggcgacgccg gcgccatggc cttcagatat aacgccgcca ttcggcgctg
     3901 ggattgccag ccatgctgca attgatgcag gcaccg
//
LOCUS       DASUEG010000973         4131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2140787, whole genome shotgun sequence.
ACCESSION   DASUEG010000973 DASUEG010000000
VERSION     DASUEG010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140787"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2233
                     /locus_tag="VFX11_18290"
     CDS             <1..2233
                     /locus_tag="VFX11_18290"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4940629.1"
                     /translation="ARMGEELVEHLSAFHFLLDLPIQNQTWNSLEPVQKRLQAQKAIR
                     ALLGFLSQHKPLILVLEDLHWIDVESQMLLEKFVEFVQSHSILLITTFRPEYQASWLS
                     DNVSLLELKPFSDLEALEYLRSILGPHTSMQQVRGEIARRCEGIPLYMEEMIHHLQDS
                     HLIWGEQGNFQSSLDTLPDSLPVTIHSVISTRIDRRSKMAKSILQAASVIGQRFSASV
                     LDYVTDIPREYARHAIAELIQAGLVVESGDPMNEEYHFKHALIHQVTYDAIPRERKRL
                     THASLVNAIESLYKERLEEHIYRLAEHAYLGQYWYKAVEYYLKACYHAIGRSSHHQAV
                     LLLDRGLDALRHLPLNQDTLGQRIDFLAVGMNALVPLGEQDRLVRDLREAEKLCAAVG
                     EPKRTCSILSQLTNALWMVGKHDEAQIAARAAVEIAQDLHYTPLMIASQYNLAMVCHA
                     QGNYEDTIAIEEKILATLSGELETRRFGWSGFPSVFARTFLGNSLVEMGKLERAEQEI
                     LRGVHVADQARHPYSQAMIYDTWGYYLNAVDRFVEARTCLDTALMLCEEYAVPTMIPA
                     VSAKLGEALIGCGELNLASKVLEAAAQPSCYRKGGRYTWFYLFKALANLHLAQNDPEQ
                     ARQYAQKAYSLTQETGEIGHHGWSSLTLARVYARQMGSRQGGAAKRQVDSLFDQALDI
                     AQQKGMHLLGFHAAREATLFNEREADVKTQLGYFDLAEYHSRFLPAKPFGAQLNAVRI
                     RVS"
     gene            complement(2230..>4131)
                     /locus_tag="VFX11_18295"
     CDS             complement(2230..>4131)
                     /locus_tag="VFX11_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940630.1"
                     /translation="FASHMRRVNPRLTLSEGESEGTLRVDQHRLIRRAMPYGPYIPTG
                     TDLAQAPDAQRGLHFFCYNARIDSQFEFVQKNWINNCDFMGFSSNVIDPIVGNRARDK
                     LGLFSFNAESLPVYGLQQYVQVKGGEYFFTPGRKGLQLLLQLVQQVDPVRVPKQRIET
                     WDPVQSDPLDVAKYVDATRLVGGNRFVKLWVQGSTGARVPYYYFAYPDDMNAILAQPN
                     VFTNVLYRERIRKLTGGDMLLSKNETPQREQQKKLTGQLLSPANFEARMQAVLGGTLE
                     QIRTGFASTGKLELVEHLARRLPLAVIKNLYGIAPPRMPADGILSRLQIAHFFDRTDY
                     NEVPQIWRENCGNYGFRTTPDDTLLFWVRMLFLQVFGNMINLDYITRLSQAAAAEFLP
                     LLDQQIDDAVARPTDPDSIMAGLIRMYRDEYGISDTDRLVKAVRQSLLELAVGSTDTT
                     AKGIAGVVAKILQFGSNLQEAISHLLADQPEALKLLQAWLANPALRLQLTPQMNGLLE
                     QVALKCLYLNPVAPLLPRFCLNGATYVSSVGETLNIEPGAMVCLVPQVTLLMELQQKL
                     PWSNGRYLFMSDSPHACMGRQIALLEIREALKLLLTLPNVRPAAGPAGMLLEKYRLPA
                     SMWLRCDVD"
BASE COUNT          829 a         1246 c         1266 g          790 t
ORIGIN      
        1 ggcacgcatg ggtgaggaac tggtggagca cctgtcagcg ttccacttcc tgctggacct
       61 gcccatccag aaccagacct ggaattccct ggagccggtg cagaaacggc tgcaggcgca
      121 aaaggccatt cgcgccctgc tgggttttct gtcccagcac aagccgctga tcctggtgct
      181 ggaggatctg cactggatcg acgtggaaag ccagatgctg ctggaaaaat tcgtggagtt
      241 cgtgcagtcc cattcgattc tgctgatcac cacgttccgc ccggaatacc aggccagctg
      301 gctgagcgac aatgtgagcc tgctggaact gaaaccgttc tccgatctgg aagcgctgga
      361 atacctgcgc tcgatcctgg gcccccacac cagcatgcag caggtgcgcg gcgagatcgc
      421 gcggcgctgc gaaggcattc ctctttatat ggaggaaatg attcaccacc tgcaggacag
      481 ccacctgatc tggggagaac agggcaactt ccagtcgtcg ctggatacgc tgccggattc
      541 gctgccggta accattcact cggtgatttc cacccgtatc gaccgccgtt cgaaaatggc
      601 gaagagcatc ctgcaggcgg cgtcggtgat cggccagcgc ttttccgcca gcgtgctgga
      661 ttacgtcacc gatattccgc gcgaatatgc ccgccacgcc attgcggagc tgatccaggc
      721 cggcctggtg gtggaaagtg gcgatccgat gaacgaggaa tatcatttca agcacgcgct
      781 gattcatcag gtgacctacg acgccattcc ccgcgaacgc aaacgcctga cgcatgccag
      841 cctggtgaac gcgatcgagt cgctctacaa ggagcgtctg gaagaacata tttaccggct
      901 ggcggaacac gcctatctgg gccagtactg gtacaaggcg gtggagtact acctgaaggc
      961 ctgttatcac gccatcgggc gctcgtcgca ccatcaggcg gtgctgttgc tggatcgcgg
     1021 tctggatgca ctgcgccatc tgccgctgaa ccaggacaca ctgggccagc gcatcgactt
     1081 tctcgcggtg ggcatgaatg ccctggtgcc gctgggcgaa caggaccggc tggtgcggga
     1141 tctgcgtgaa gcggaaaaac tctgtgcggc cgtgggcgaa cccaagcgca cctgttccat
     1201 tctgagccag ctgaccaatg cgttgtggat ggtaggcaaa catgacgagg cgcaaatagc
     1261 cgcccgcgct gccgtggaaa ttgcccagga cctgcattac acaccgttga tgatcgccag
     1321 ccagtacaac ctggcgatgg tctgccacgc tcagggcaat tacgaggaca cgattgcgat
     1381 cgaggaaaaa atcctggcca cgctgtcagg cgaactggaa acccgccgct tcggctggtc
     1441 cggttttccc agcgtgttcg cccgcacttt cctgggcaac agcctggtgg agatgggcaa
     1501 gctggaacgg gcggagcagg aaatcctgcg cggcgtgcac gtggccgatc aggcccgcca
     1561 tccttactcc caggccatga tctatgacac ctggggctat tacctgaatg cagtggaccg
     1621 gtttgtggaa gcacgtacct gtctggatac ggcgctgatg ctgtgcgagg aatacgcggt
     1681 gcccaccatg attccggcgg tatcggccaa gctcggcgaa gcgctgatcg gctgcggtga
     1741 actgaatctg gccagcaagg tgctggaggc tgccgcacaa cccagttgtt accgcaaggg
     1801 tggccgctac acctggtttt atctgttcaa ggcactggcg aacctgcatc tggcgcagaa
     1861 cgatccggag caggcacgcc agtacgcgca gaaggcctac agtctgacgc aggaaaccgg
     1921 cgagatcggg catcacggct ggtcgtcgct gacgctggcg cgggtgtacg cgcggcagat
     1981 gggaagccgt cagggtggtg ctgccaaacg ccaggtggac tcactgttcg atcaggcgct
     2041 ggatatcgcg cagcagaaag gcatgcacct gctgggcttc catgccgcgc gcgaggccac
     2101 gctgttcaat gaacgggaag cggatgtgaa aacccagctg ggctatttcg acctggccga
     2161 gtatcacagc cgcttcctgc ccgccaaacc ctttggcgca cagctgaatg cagtgcggat
     2221 ccgggtatcc taatcaacgt cacaccgcag ccacatgctg gccggtaacc ggtatttctc
     2281 cagtaacata ccggccgggc ctgcggcagg acgcacattt ggcaacgtca gcagcaactt
     2341 cagcgcctcg cggatttcca gcagcgcaat ctgccggccc atgcaggcat gcggcgaatc
     2401 ggacatgaac aggtagcggc cattgctcca gggcagtttc tgctgcagtt ccatcagcag
     2461 ggtcacctgg ggcaccagac acaccatggc gcccggttcg atattgaggg tctctcccac
     2521 tgaactgacg taggttgcgc cattcaggca gaagcgaggc agcaacggcg ccaccggatt
     2581 cagatacaga cacttcagcg ccacctgctc cagcaacccg ttcatctgcg gcgtcagttg
     2641 cagccgcagc gccgggttgg caagccaggc ctgcagcagt ttcaatgcct cgggctgatc
     2701 ggccagcaga tggctgatgg cctcctgcag gttggatccg aactgcagta ttttggccac
     2761 cacgccggcg atgccttttg ccgtggtatc ggtactgccc accgccagtt ccagcaggct
     2821 ctgccgcacc gctttcacca gacgatcagt gtcgctgatc ccgtattcgt cacggtacat
     2881 ccggatcagg cccgccatga tgctgtcggg atcggtcggg cgggccacag cgtcgtcaat
     2941 ctgctggtcc agcagcggca ggaattcggc tgccgccgcc tgtgacagcc gggtgatgta
     3001 gtccaggttg atcatgttgc cgaacacctg caggaacagc atgcgcaccc agaacaggag
     3061 cgtatcgtcc ggcgtggtgc ggaaaccgta gttgccacag ttctcgcgcc agatctgcgg
     3121 cacctcgttg taatcggtgc gatcgaagaa gtgggcaatc tgcaggcgcg acaggatgcc
     3181 atctgccggc attcgtggcg gtgcaatgcc atacaggttc ttgatcacgg ccagcggcag
     3241 ccgccgcgcg agatgttcca ccagctccag tttgccggtg ctggcgaatc cggtacgaat
     3301 ctgctccagc gtgccgccca gcaccgcctg catgcgggcc tcgaaattcg ccggggacag
     3361 cagctggccg gtgagtttct tctgctgttc ccgctgcggg gtttcatttt tcgacagcag
     3421 catgtcgccg ccggtgagtt tgcggatacg ctccctgtac agcacgttgg tgaacacgtt
     3481 cggctgcgcc aggatcgcgt tcatgtcgtc gggataggcg aagtaatagt aaggcacgcg
     3541 cgcgccagta ctgccctgca cccacagctt gacgaagcgg ttgcctccca ccagacgggt
     3601 ggcgtccaca tatttcgcca catccagcgg atcggactgc accgggtccc aggtttcgat
     3661 gcgctgtttg ggcacccgca ccggatccac ctgctgcacc agttgcagca acagttgcag
     3721 gcccttgcgt cccggcgtga aaaaatactc gccgcctttc acctgcacat attgctgcaa
     3781 gccgtacacc ggcagggatt cagcattgaa cgagaacagg cccagcttgt cgcgggcgcg
     3841 gttgccgacg atgggatcga tcacgttgga ggaaaagccc atgaaatcgc aattattgat
     3901 ccagttcttc tgcacgaatt caaactggga atcgatgcgg gcgttgtaac agaaaaaatg
     3961 caggccgcgc tgggcgtccg gcgcctgggc aaggtcggta ccggtgggaa tatagggtcc
     4021 ataaggcatg gcacggcgaa tcaggcggtg ctggtccacc cgcagggtac cctcgctctc
     4081 gccttcactc agggtcaggc gcggattcac ccgccgcatg tgggatgcga a
//
LOCUS       DASUEG010000974         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1023079, whole genome shotgun sequence.
ACCESSION   DASUEG010000974 DASUEG010000000
VERSION     DASUEG010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023079"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFX11_18300"
     CDS             complement(<1..561)
                     /locus_tag="VFX11_18300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4940631.1"
                     /translation="MDEPLDPLIGRILNNAFRIESLLADGGMSRVYLAHQISLARTVV
                     IKMLLPGFNDPDFIQLFLREARVNSNLNHPNIVGVFDFGQTEDGLVFLAMEYLEGATL
                     ADVVQGQGDGQKGLTLANIVWLMEQLCNAVSAAHQQQVVHRDLKPGNVMVARLAGNTT
                     VVKVVDFGISKPMDEENLKHTQMGAVM"
     gene            complement(554..2209)
                     /locus_tag="VFX11_18305"
     CDS             complement(554..2209)
                     /locus_tag="VFX11_18305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4940632.1"
                     /translation="MQNETTMWGKVRAPGFSVQASRYALTIAYHEDWQRIGATAYLDD
                     LSKGKSVAVSRLEPEFQLPFLSGGYLLDDRYVSRKPLTIEQLNGRLLLTPHPEGSEVR
                     VDGQVIRAHHYVFEEVLQQGVVIELGQRVVLVLHSLAPELRSKNMHRMLGVSEAIESV
                     RRSINRVADLDVPVLIRGETGTGKELVASAIHNASLRSQKPLLAVNVAAIPDSLAISE
                     LFGARKGSFTGAARDKPGYFVQADNSTLFLDEIGDAPDEVQVALLRTLETGEVRPVGA
                     TDSVKVDVRMIAATDANLESKLEAQTFRNPLLQRLAGFEIWLSPLAQRKADIGLLLAE
                     FLRFEFDAIGEQRHYQLGAEATAGTDNLWYRFFRQALEFPWPGNIRQMRNVARQVAIH
                     NRQAMVTSIPFRIEAMLAETRPAPETAGGALHSVSDGASNGVANTAPGPAPVSDDAHD
                     DDDEPGIPRRKPSTVTEAELMSMLEQQRWDLKNTAEHLNISRAALYKMIEANPRVRKA
                     GDISVEEIQQSYVRCGGNLERMVEELKVSGAALKRRIRELGIG"
     gene            complement(2283..2585)
                     /locus_tag="VFX11_18310"
     CDS             complement(2283..2585)
                     /locus_tag="VFX11_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX4940633.1"
                     /translation="MGQPAARVGDMHVCPMMTPGVPPIPHVGGPIAAPSAPTVLIGGM
                     PAAGMGSLCTCVGPPDSIVKASMTVMIGGKPAARMGDMTAHGGSIVIGCPTVLIGG"
BASE COUNT          519 a          888 c          745 g          549 t
ORIGIN      
        1 catcaccgcg cccatctgcg tgtgcttgag gttttcctca tccatcggct tgctgatgcc
       61 gaaatccacc accttcacca ccgtggtgtt acccgccagc cgcgccacca tcacattgcc
      121 gggcttcaga tcgcgatgca ccacctgctg ctgatgcgcg gcgctgactg cattgcacag
      181 ctgttccatc aaccagacga tattggccag ggtcagacct ttctgcccat caccctgccc
      241 ctgcaccaca tccgccaggg tcgccccttc caggtattcc atcgccaaga acaccagacc
      301 gtcttcggtc tggccgaagt cgaacacacc gacgatattg ggatgattga ggttgctgtt
      361 gacgcgggct tcacgcagga agagctggat gaaatcggga tcgttgaagc ctggcaacaa
      421 catcttgatg acgacggtgc gcgccaggct gatctggtgc gccagataca cgcgactcat
      481 accgccatcg gccagcaggc tttcgatgcg aaaggcgttg ttgagaatgc ggccgatcag
      541 gggatccagc ggctcatcca atccccagct cccggatgcg acgcttgagt gcggcgccag
      601 acactttcag ttcttccacc atgcgctcca gattgccgcc gcagcggacg tatgactgct
      661 ggatttcctc gacgctaata tccccggcct tgcgcacacg gggattggct tcgatcatct
      721 tgtacagggc agcgcgggaa atattcaggt gttcagcggt gtttttcaga tcccagcgct
      781 gctgttccag catagacatc aattccgctt ccgtcaccgt gctgggcttg cgccggggaa
      841 taccaggctc gtcgtcatcg tcgtgcgcat cgtcagatac aggcgctggc ccgggagcgg
      901 tgttggcaac accattggat gcgccatcgg acacggagtg caacgcaccg ccagctgttt
      961 ccggcgccgg ccgggtctcc gccagcatgg cttcaatacg gaatggaatg ctggtcacca
     1021 tcgcctgccg attgtgaatc gccacctgcc gcgccacgtt gcgcatctgg cggatattgc
     1081 cgggccacgg gaattcgagt gcctgacgga aaaagcgata ccacaggttg tcagtaccgg
     1141 cggtggcttc cgcgcccagt tgatagtggc gctgctcgcc gatggcatcg aactcgaacc
     1201 gcaggaattc cgccagcagc aggccgatat ccgccttgcg ctgagccagg ggcgaaagcc
     1261 agatttcgaa ccccgccagc cgctgcaaca acggattgcg gaaagtctgt gcctccagct
     1321 tgctttccag attggcatca gtggccgcga tcatgcgcac atccaccttc acgctgtcgg
     1381 tggcacccac cgggcgcacc tcaccggttt ccagcgtgcg caacagggcc acctgcactt
     1441 cgtccggtgc gtcgccaatt tcatccagga aaagcgtgct gttgtcggcc tgcacgaaat
     1501 agccgggctt gtcgcgggcg gcgccggtga aagaaccctt gcgcgcaccg aacagttccg
     1561 agattgccag cgagtcggga atggccgcca cgttcactgc cagcagcggc ttctggctgc
     1621 gcagactggc attgtggatt gcactggcca ccagttcctt gccggtaccg gtttcaccac
     1681 gaatcagcac cggcacatcc agatcggcca cccgattgat ggaacgacgg acactttcaa
     1741 tggcctcgct cacgcccagc atgcgatgca tatttttgct gcgcagctcc ggcgccaggc
     1801 tgtgcagcac cagcaccacc cgctgaccca attcgatcac cacaccctgc tgcagcactt
     1861 cctcgaacac ataatggtgg gcacggatca cctgcccatc cacccgcact tccgaacctt
     1921 cgggatgcgg cgtcaacagc aggcggccgt tgagctgttc gatggtgagg ggtttacggc
     1981 tgacataacg gtcatccagc agataaccgc cggacaggaa cggcaactgg aattccggct
     2041 ccagccgcga cacggccacg cttttcccct tcgacaaatc atccaggtag gcggtagcgc
     2101 caatacgttg ccaatcctcg tgatatgcga tcgtcaaagc atagcggctc gcctggacac
     2161 tgaagccagg cgcgcgcact ttgccccaca tggtggtttc attctgcatt cacttccccg
     2221 gctggctgat ttttattttc ccgtgaatgg aagatgtatt ctgccaaatc cagcgccagc
     2281 gttcacccgc ctatcagcac cgtcggacac ccgataacga tggacccacc atgcgccgtc
     2341 atgtcaccca tgcgcgctgc cggcttgccg ccgatcatca ccgtcatgct ggctttgacg
     2401 atgctgtccg gcgggccgac gcaggtgcaa aggctgccca tgcccgccgc tggcataccg
     2461 ccaatcaata ccgtcggcgc agacggtgct gcaataggcc cgcccacatg aggaattggc
     2521 ggtacacccg gtgtcatcat cgggcagaca tgcatgtcgc ccacgcgcgc tgcgggttgt
     2581 cccatgatcc agcctccgtc agttgatcat caccaggccg ccctgcattt tcagcatgcc
     2641 gccgcctttc acttccgtca tcgcgttgcc gttgattttt accatggtgc cggtgatggt
     2701 c
//
LOCUS       DASUEG010000975         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1450127, whole genome shotgun sequence.
ACCESSION   DASUEG010000975 DASUEG010000000
VERSION     DASUEG010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450127"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFX11_18315"
     CDS             complement(<1..632)
                     /locus_tag="VFX11_18315"
                     /inference="COORDINATES: protein motif:HMM:NF019316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEX4940634.1"
                     /translation="MLMCHFRWIFPPLLRACLLALACLCAAPPALALQPLAIDPGFNE
                     TTLGRQADFILDDSRQLTLDAAMAEDAPWQPLQMDTASFGFNARPLWLKLELASGSPQ
                     SRHLLLIVDYPVLDRVDIHVVHPGKPPERVTVGDTLPFDQRPRDHRLFLLPLTLEPQA
                     RMTLYLRVQTEGSLQVPLRLADREYFYEHQLGFTMIQGWYFGIMTVMALY"
     gene            complement(755..3283)
                     /locus_tag="VFX11_18320"
     CDS             complement(755..3283)
                     /locus_tag="VFX11_18320"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940635.1"
                     /translation="MRGRLPLPSLLYLLLAACLLLGNALPAQALELVLAEGVPFRAQN
                     AATGIEYLRDPTGTLALEEVQQQPASAWALSQADVPSFGFDAAAYWLRLQVRNDTASH
                     QSLVLDIAYPILDDIQAHIRYSNSGTATFHLGDTLPFASRPIDHHNFAVPLKLDAGTT
                     ASIYLRVQTGTSMQIPLTLWDREHFFEQQQRYLMGQGLYFGILLVMAVYNLFIYVSVR
                     HSAYIYYAISVMGMAFFMASLHGFGFQYVWPEFPGVNNWVTTVSLSLFVGAAAAFTNA
                     LLQMRKNSPRYYYVLLATALVYAALILLTPWLSYAFRIKVGTALGVVSSLTALAAGSY
                     LLHKGVRTARYYVMAYSFLVFGFVALSLNKFGLLPRNTLTEFSAQICSVIEIVLLSFA
                     LADRINEERREKYKAKQQALEHEQRAREEQERYLRLRHSAELEELQAQQKIIAAQAES
                     RAKSEFLATMSHEIRTPMNGVLGMAELLQDTDLQPQQRQYLDVITSSGKALLHIINDI
                     LDYSKISAGKLALEQIEFDLAQLCRDCSAMFAATAERKQIALNCTLEEGTPLRIQADP
                     TRLRQILLNLLGNAFKFTPQGEIRLRVLALSPVNASGQTRLQFEVSDTGIGISAEAQA
                     RLFQEFAQADSSITREYGGTGLGLSISQRLVALMGGEIGVFSEPGKGSTFWFTLPCLA
                     INASANNSSVPAVATDHQHPRLRGRRVLVVEDNPVNLLVINGMLKKLGMICETADNGQ
                     TALDLLRDHHTRFDLVLMDCEMPVLDGYAAARALRDHERQQQLSPLPVIALSAHVLPE
                     HQDRARDCGMNDHLAKPLEFSALKTKLAICLGSTPVASQLGLAH"
BASE COUNT          628 a         1021 c         1097 g          617 t
ORIGIN      
        1 ttgtacagcg ccatcaccgt catgatgccg aagtaccagc cctggatcat ggtgaagccc
       61 agctggtgct cgtagaagta ttcccggtct gccagccgca gcggaacctg cagagagccc
      121 tcggtctgta cccgcaggta cagggtcatg cgggcctggg gttccagtgt cagtggcagc
      181 aggaacaggc ggtgatccct tggccgctgg tcgaaaggca gggtgtcgcc gacggtcacg
      241 cgctccggcg gcttgccggg atggacgacg tggatgtcga cccggtccag caccgggtaa
      301 tccacgatca gcaacaggtg tcgcgattgg gggctcccgc tggccagttc cagtttcagc
      361 cagagcggtc tggcgttgaa gccgaatgac gcggtatcca tctgcaaggg ctgccagggg
      421 gcatcttccg ccatggccgc gtccagggtc agctggcggc tgtcgtccag tatgaaatcc
      481 gcctggcgcc ccagggtggt ttcattgaag ccgggatcga tcgccagggg ttgcagcgcc
      541 agggcaggcg gggccgcaca taggcaggcc agtgccagca ggcaggcacg cagcagaggc
      601 ggaaagatcc agcggaaatg gcacatcaac atcacaacgg ggattcacca gtcaggcagc
      661 cctctacact ataggccagc caccccatcc cgagcgggct tatcgcattt tgatatgaat
      721 cctgcgcggg gtatgtccgg caaggtgtgg gtgttcagtg ggccaggccc agctggctgg
      781 ccaccggcgt gctgcccagg caaatggcca gcttggtttt cagggcgctg aattccagcg
      841 gcttggccag atgatcgttc atgccgcagt cccgtgcccg gtcctggtgc tccggcagca
      901 catgggcgct gagggcgatc accggcagcg gcgacagctg ctgttgccgc tcatgatcgc
      961 gcagggcgcg ggcggcggca tagccgtcca gcaccggcat ctcgcagtcc atcagcacca
     1021 gatcgaagcg ggtgtgatga tcccgcagca gatccagcgc ggtctggccg ttgtccgccg
     1081 tctcgcagat catgcccagc tttttcagca tgccgttgat caccagcagg ttcaccggat
     1141 tgtcttccac caccagcacc cggcggcccc gcaggcgcgg gtgctggtgg tcggttgcga
     1201 cagccggaac actgctgttg ttggcgctgg cgttaatggc cagacagggc agggtgaacc
     1261 agaacgtgct gccttttccc ggttcgctga aaacgccgat ttcgccgccc atcaacgcta
     1321 ccagccgctg cgagatgctc aggcccagcc cggtgccgcc gtattcccgc gtaatggacg
     1381 aatcagcctg ggcgaattcc tggaacaggc gcgcctgggc ctcggcggaa atgccgattc
     1441 ccgtgtcgct cacttcgaac tgcagccggg tctggccgct ggcattgacg ggtgacaggg
     1501 ccagcacccg cagccggatt tcgccctggg gcgtgaactt gaatgcgttg cccagcaggt
     1561 tcagcagaat ctggcgcagg cgggtgggat ccgcctggat gcgcagcggt gtgccctctt
     1621 ccagcgtgca gttcaatgcg atctgcttgc gttcggcggt ggcggcaaac atggcgctgc
     1681 agtcgcgaca caattgggcc aggtcgaatt cgatctgctc cagggccagc ttgccggcgc
     1741 tgattttcga gtaatccaga atgtcgttga tgatgtgcag cagcgccttg ccggaactgg
     1801 tgatcacatc cagatattgc cgctgctgcg gttgcaggtc ggtatcctgc agcagttcgg
     1861 ccatgcccag cacgccgttc atgggggtgc ggatttcatg gctcatggtg gcgaggaact
     1921 cgctcttggc acggctttcc gcctgggcgg cgatgatttt ctgctgggcc tgcagttcct
     1981 ccagctcggc gctgtggcgc aggcgaaggt agcgttcctg ttcttcgcgg gcgcgctgtt
     2041 cgtgttccag tgcctgctgc ttggccttgt atttctcgcg ccgttcctcg ttgatgcggt
     2101 ccgccagcgc gaacgacagc agcacgatct cgatcaccga acagatctgg gcgctgaatt
     2161 cagtgagcgt attgcgcggc agcaaaccga acttgttgag cgacagcgcc acgaagccaa
     2221 ataccaggaa gctgtaggcc atcacgtaat agcgggcagt gcgcacaccc ttgtgcagca
     2281 gatagctgcc ggcggccagc gccgtcagcg agctcaccac tcccagcgcg gtaccgacct
     2341 tgatgcggaa agcgtacgac agccagggcg tcagcaggat cagggcggca tacaccagcg
     2401 ccgtggccag cagcacgtag taatagcgcg ggctgttctt cctcatctgc agcaaggcat
     2461 tggtaaatgc cgcggcggcg cccacgaaca ggctcagcga cacggtggtg acccagttat
     2521 tcacgccagg gaattccggc cacacatact ggaaaccgaa accgtgcagc gacgccatga
     2581 aaaacgccat ccccatcacc gagatcgcat agtagatata ggcgctgtga cgcaccgaaa
     2641 catagatgaa caggttgtac accgccatca ccagcaggat gccgaaatac agaccctgac
     2701 ccatcagata gcgctgctgc tgttcgaaga aatgctcccg gtcccacagg gtgagcggga
     2761 tctgcattga tgtgcctgtc tgcacccgca ggtagatcga ggcggtggtg cccgcgtcca
     2821 gtttcagggg cacggcgaaa ttgtggtggt cgatggggcg gctggcgaaa ggcagggtgt
     2881 cgcccaggtg gaaggttgca gtcccgctgt tgctgtagcg gatgtgggcc tgaatgtcat
     2941 ccaggatcgg atacgcgata tccagcacca gggactggtg gctggcggta tcgttgcgca
     3001 cctgcagccg cagccagtag gcggccgcgt cgaatccgaa ggagggcacg tccgcctgcg
     3061 acagggccca ggccgaggcc ggttgttgct gcacctcttc cagggccagt gtgccggtgg
     3121 ggtcgcgcag gtactcgatg ccggttgcgg cgttctgcgc gcggaacggc accccttccg
     3181 ccagcaccag ttccagtgcc tgcgcgggca atgcgttacc cagcagcagg catgccgcca
     3241 gcagcaggta cagcagagaa gggagcggga ggcgacctcg cacccggcgg gaatccggcg
     3301 cagacaaggc aataatccaa tagcaatgca acgctatgac tatagcctat gcccctgcat
     3361 ccc
//
LOCUS       DASUEG010000976         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1757579, whole genome shotgun sequence.
ACCESSION   DASUEG010000976 DASUEG010000000
VERSION     DASUEG010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757579"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="VFX11_18325"
     CDS             complement(<1..2057)
                     /locus_tag="VFX11_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4940636.1"
                     /translation="MFLKKMLYVNWGNIPQLELDFGPVNLFSGGNGSGKTTAADGIQT
                     LMTAAYENLFNFNPGQDETTQRGRGGKQVRTLASYVLGCDDGSYARLSTTDGYLGAVF
                     APGRGEKAEPFTILMGMRAHLDSAGNQRQARLDDVQFFILPATELSIDKLVRSYNDGK
                     HVVPLHELTDLLQREFGKKALEVYDKKGAYLRRLYGLLRGKRDAVSDREAKHAARTFA
                     NFMAYKPVKSINDFVAEEILEKKDLGEAIRTVSDLMKTIHGMDEDARHIRESLGILQQ
                     VSATSSHYVEHWILRAVLRYQEASRLFQLKQKAYIDTKQLQKKLQDDIQERETRLAVN
                     RERRAETHQQIVELEAQRRGISVLNEKDQLQATITKLQHALTGKAAPLLQQDQQLRRN
                     LDSTKQIAASLQQQSWGTDIAALDQKPLRESLRAVLKLEQTHNIDLQRLFNRDWVDIA
                     PLEEYLDRAIELDTLHNQWSDRLHLPVSEQVASVRDQLAALKNEWERELQSQQRQLQQ
                     KEKEIAQLEARKVSYPPGVEVALKAIRAQCPDADPRVLCDYIDVLDPQWQMAIEGYLG
                     GARFSIIVKPEYEAEAIRIVRNLPGQRNSARVIQGSKAQRDAERFQPAADSIIHILKF
                     QHKTAEYYLRASYGSVVRVPDAETLRSTARGITSNGLASGNYSMWRCDIPDAELVFGQ
                     SAR"
     gene            complement(2061..2792)
                     /locus_tag="VFX11_18330"
     CDS             complement(2061..2792)
                     /locus_tag="VFX11_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEX4940637.1"
                     /translation="MNLSELLQQKLSAKQFSTDEFSELLIRLLDYGVICRDESQIEEQ
                     LYDRYLQLHDLVEDYLSPLRVRIQHDRRFCFVRIFPPGAQVPGLPDDDAQPFSGGLRA
                     QLSQQEVATVLVLRAEYDKALREGQIDEQGSVMLSLEALGIALKNLLNRTLPEKKTER
                     AQLFRRLRQLRLIQFSQGSDLMQQDAWLKIRPSITSFVNETILQNLGAGLAGTAPAED
                     ADSLDEDASEMSGKPASTLFAVGEE"
     gene            complement(2803..4272)
                     /locus_tag="VFX11_18335"
     CDS             complement(2803..4272)
                     /locus_tag="VFX11_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetA family
                     protein"
                     /protein_id="HEX4940638.1"
                     /translation="MFFSDSRNQFFRPLVSKYREQIVECLRLLYERQYSESADYGESL
                     QRDQVVEIFEEALARAPLLDSDDSDADTRFKSEREQANWILNQLLEHGWLEKQVDQAT
                     LHSTFPFSRMGRLFTQSFSELGRASVRTRHRNTRNTLNALEAFVNRGEVHDLLDAYEY
                     SERIISDFTDVIAELEERKRELVREVEARMLVQQATDHFFEFMEKRFQPDLSVRLSAD
                     SVEKHRDAVQDVIDRIRRKRKEFKHDAEKKLRQLAPELVEQLAQPGRSVLYFVLDTIE
                     DRMRRAADLMLPALRRALQSFTKRADIIIRQLSYLNAQRDNDVVEVCRQLAQLPETDQ
                     NARLEQAGRELAPLRVQLIDPEQVRLTQRREQMPLDTRVEEEQAVSEQNRREMLVQNL
                     LDQAFVMHNEGLRDYVVQSMAGGSRISTADLPVHNARELLAMAHAIETASHQHFDSDY
                     RFQVEYAGGQGKNNYFDTFDIFHIELVRGSDTAPVPASE"
     gene            complement(4564..>4895)
                     /locus_tag="VFX11_18340"
     CDS             complement(4564..>4895)
                     /locus_tag="VFX11_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4940639.1"
                     /translation="AVPSLAAPQDLTLYSFESSPYSRIVREKLSELELPYKLVNIGKQ
                     QRADVGPATRRLHLGPYKPLPNTKRSEFFAKHGNVQVPYLIDPNTGIDMFESADISAY
                     LDKTYKR"
BASE COUNT          900 a         1475 c         1445 g         1075 t
ORIGIN      
        1 tcgcgtgcac tctggccgaa gaccagttct gcatcgggaa tgtcgcagcg ccacatgctg
       61 taattaccgg acgccagacc attgctggta attccgcgcg cggtggagcg caatgtttcc
      121 gcatccggca cccgcaccac gctgccgtag ctggcacgca gataatattc ggcggttttg
      181 tgctggaatt tcagaatgtg aatgatggaa tccgccgccg gctggaaccg ttcggcatca
      241 cgttgcgcct tgctgccctg aatcacccgc gcgctgttgc gctggcccgg caggttgcgc
      301 acgatgcgga tcgcctcggc ctcgtattcc ggcttcacga taatggaaaa ccgcgcgcca
      361 cccagatagc cttcgatggc catctgccac tgtggatcga gcacgtcgat gtaatcgcac
      421 aatacccgtg gatcggcatc ggggcactgg gcgcgaatgg ctttcagcgc cacttccaca
      481 ccgggtggat aggacacctt gcgcgcttcc agttgcgcga tttccttttc cttctgctgc
      541 aactgacgct gctgggactg cagttcgcgc tcccattcgt tcttcaatgc cgccagctga
      601 tcgcgcacgc tggcgacctg ttccgacacc ggcagatgca accggtcgct ccattgattg
      661 tgcaacgtat ccagttcaat ggcgcggtcg aggtattctt ccagcggtgc gatatccacc
      721 cagtcgcgat tgaacaggcg ctgcagatca atgttgtggg tctgctccag tttcagcacg
      781 gcacgcaggg actcccgcag cggcttctgg tccagcgcgg cgatgtcggt gccccaggat
      841 tgctgctgca ggctggcggc gatctgtttg gtgctgtcca gattgcggcg taactgctgg
      901 tcctgctgca gcaagggtgc agccttgccg gtcaatgcat gctgtaattt ggtgatggtg
      961 gcctgcagct ggtccttttc attcagcacc gatatgccac gacgctgcgc ttccagctcc
     1021 acgatctgct ggtgggtttc tgcccggcgt tcacggttca ccgccagccg ggtttcacgt
     1081 tcctggatat cgtcctgcag tttcttctgc agttgtttgg tatcgatata ggctttctgt
     1141 ttcagctgga acaggcggct ggcttcctgg tagcgcagaa cggcgcgcag aatccagtgt
     1201 tcgacataat gactgctggt ggcagacacc tgctgcagga tgccaaggga ttcacggata
     1261 tgacgtgcgt cctcgtccat gccgtgaatg gttttcatca ggtcggacac ggtgcggatc
     1321 gcttcgccca gatccttttt ctccaggatt tcctcggcga caaaatcgtt gatgcttttt
     1381 accggcttgt aggccatgaa attggcaaag gtgcgcgccg cgtgtttggc ttcgcggtcc
     1441 gacacagcat cgcgcttgcc ccgcagcagg ccatacagcc ggcgcagata ggcacctttc
     1501 ttgtcgtaga cctccagcgc tttcttgccg aattcgcgct gcagcaggtc ggtcagttcg
     1561 tgaagcggca ccacatgctt gccgtcgtta taactgcgca ccagcttgtc aatgctcaac
     1621 tcggttgccg gcagaatgaa aaactgcacg tcgtcgagcc gcgcctgccg ctggttgccg
     1681 gcgctgtcca ggtgcgcgcg catgcccatc aggatggtga agggttcggc cttttccccg
     1741 cgcccgggcg cgaacacggc gcccagataa ccgtcggtgg tggacagccg cgcataactg
     1801 ccgtcatcgc agcccagcac atacgacgcc agcgtgcgca cctgcttgcc gccacgacca
     1861 cgctgggtgg tttcatcctg gcccgggttg aagttgaaca ggttttcgta agctgcggtc
     1921 atcagggttt gaatgccgtc cgccgccgtg gtcttgccgg aaccgttgcc accggaaaac
     1981 aggttcaccg ggccgaagtc cagttccagt tggggaatgt tgccccagtt cacgtagagc
     2041 atttttttca gaaacatgcg ttattcctcg cccacggcaa acagggtgga tgcaggcttg
     2101 cctgacattt ccgacgcatc ttcgtcgagg gaatcagcat cctcagccgg agcagtaccg
     2161 gcaagacccg cccccagatt ctgcagaatg gtttcattga cgaagctggt gatcgagggc
     2221 cggattttca gccaggcatc ctgctgcatc agatcgctgc cctggctgaa ctggatcagc
     2281 cgcaactggc gcaaacgccg gaacagctgc gcgcgctcgg ttttcttttc cggcaaggtg
     2341 cggttgagca gattcttcag cgcaataccc agcgcttcca gcgacagcat taccgaaccc
     2401 tgctcgtcga tctgcccttc ccgcagcgcc ttgtcgtatt cggcgcgcaa caccagcact
     2461 gttgccactt cctgctggct cagctgcgcc cgcagaccac cgctgaacgg ctgggcgtcg
     2521 tcgtccggca atcccggcac ctgcgcaccg ggcggaaaaa tccgcacgaa gcagaaacgc
     2581 ctgtcgtgct ggatacgcac ccgcagcggt gacagatagt cctccaccag atcgtgcaac
     2641 tgcaggtagc ggtcgtacag ctgttcctcg atctggcttt catcccggca gatcacgccg
     2701 taatccagca gccggatcag cagctcactg aattcatcgg tactgaactg tttggcactg
     2761 agtttctgtt gcagcaattc ggataggttc attctggctg tttcattcag atgcaggaac
     2821 cggtgcggta tctgaaccgc gcaccagttc gatatgaaaa atgtcgaacg tgtcgaagta
     2881 attgttcttg ccctgcccgc cggcgtattc cacctggaaa cggtaatcgc tgtcgaaatg
     2941 ctggtgcgac gcggtttcaa tggcgtgcgc catggccagc agttcgcggg cattgtgcac
     3001 cggcagatcg gcggtactga tgcggctgcc accggccatc gactgcacca cataatcgcg
     3061 cagaccttcg ttgtgcatga caaacgcctg gtccagcaga ttctgcacca gcatctcgcg
     3121 ccggttctgc tcgctgacgg cctgctcttc ttccacccgg gtatccagtg gcatctgctc
     3181 gcgccgctgg gtcaggcgca cctgctcggg gtcgatcaat tgcacccgca acggcgccag
     3241 ttcccgcccc gcctgttcca gccgtgcgtt ctggtccgtt tccggcagtt gcgccagctg
     3301 gcggcagact tccaccacgt cgttgtcgcg ctgggcattc agatacgaca gctggcgaat
     3361 gatgatgtcg gcccgcttgg tgaaactctg cagtgcgcgg cgcaatgccg gcagcatcag
     3421 atcggcggcg cggcgcatgc ggtcttcaat ggtatcgagc acgaaataca gcaccgaccg
     3481 gcccggctgc gccagctgct caacaagttc aggcgccagc tgccgcagtt ttttctcggc
     3541 gtcgtgcttg aattccttgc gcttgcggcg gatgcggtcg atcacgtcct gcaccgcgtc
     3601 gcggtgtttt tccacgctgt cggcggacag gcgtacggac agatccggct ggaaccgctt
     3661 ttccatgaat tcaaagaaat gatcggtggc ctgctgcacc agcatgcgcg cttccacttc
     3721 ccgcaccagt tcacgcttgc gctcttccag ctcggcaatc acgtcggtga aatcgctgat
     3781 gatgcgctcg gaatattcat aggcgtcgag cagatcatgc acttcgcccc ggttcacaaa
     3841 ggcttccagc gcgttcagcg tgttgcgggt gttgcggtga cgggtgcgca ccgaggcacg
     3901 gcccagttcc gaaaagcttt gcgtgaacag acgccccatg cgcgaaaacg gaaaggtgct
     3961 gtgcagcgtg gcctggtcca cctgcttttc cagccagccg tgttccagca gctggttcag
     4021 aatccagttg gcctgttcgc gctcggattt gaagcgcgtg tcggcgtcgc tgtcatcgct
     4081 gtccagcagg ggcgcgcggg ccagcgcttc ctcgaaaatc tccaccacct ggtcgcgctg
     4141 cagcgattcg ccgtaatcgg cactttcgct gtactggcgc tcgtacagca ggcgcaggca
     4201 ttcgacgatc tgttcacggt acttgctgac caggggacgg aaaaactggt tgcggctgtc
     4261 gctaaaaaac ataggttgca ctaaaaaaca taggcaggaa tcgggttacc ggcagagatg
     4321 acaggaagaa tgacgccaaa cccggtgcag gacaacggtt gcggcaactg accgcctttt
     4381 tactggaatt cgcggggtac ttcaagcaag cagacagggc cggaactgtc cagaaggaac
     4441 tggaaaaaaa cctgtcttat caggcagctg cacaaactgt agaagccagg ggctgcaacg
     4501 tgtcgtcagg tccggagggg gcaggtcttt cactatcagg tccgccccgc tccggtgcca
     4561 gtgtcagcgc ttgtaggtct tgtccagata cgcggagata tcggcggact cgaacatgtc
     4621 gatgccggtg ttgggatcga tcaggtaagg cacctgcacg ttgccgtgtt tggcaaagaa
     4681 ttccgaacgc ttggtgttgg gcagcggctt gtaagggccc aggtgcaaac ggcgggtggc
     4741 tggccccaca tccgcccgct gctgtttgcc gatgttgacc agcttgtagg gcaattccag
     4801 ctcgctgagt ttttcccgca cgatgcgcga gtaagggctg gattcgaagc tgtacagggt
     4861 caggtcctgc ggtgccgcca gcgagggcac ggcac
//
LOCUS       DASUEG010000977         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1317541, whole genome shotgun sequence.
ACCESSION   DASUEG010000977 DASUEG010000000
VERSION     DASUEG010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317541"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="VFX11_18345"
     CDS             complement(<1..1871)
                     /locus_tag="VFX11_18345"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX4940640.1"
                     /translation="MVTVRNNPVVREDGSIDLRAWIEHLKEKVEITDEEGLLQLAEYS
                     RDLKNKTPNSQNAWAESASSFMTGLEMVEILADLRMDTDSLKAAVIYRAVRERQTTLE
                     NVSRMAGTGIAKLIEGVLRMAAIAAVMNPEKKVVLGQATDQLDNLRKMLVAMVDDVRV
                     ALIKLAERTCAIRAVKTAPEDKKQKVAREVFDIYAPLAHRLGIGHLKWELEDLSFRYL
                     QPEAYMRIAKLLDEKRMDRDNYIRNVVKQIHDALARVGIEAEISGRAKHIYSIWRKMK
                     RKNIDFYQVYDIRAVRVLVPEIRDCYAALGVAHNLWQHIPKEFDDYIATPKENGYRSL
                     HTAVLGPEGKVLEVQIRTQDMHDEAELGVCAHWRYKEGSTGRTDSYDDKIAWLRQVLE
                     WQEELGDSAVSSVLSQFSEDIIDERVYVFTPDGHVVDLAAGATPLDFAYHVHTEVGHR
                     CRGAKVNGRIVPLNYKLQTGEQVEILKGNEARPSRDWLQPSLGFIGTSRARAKVVHWF
                     KQQDRGRNIEDGREVLEKEFKRLDVGKINLEPLAQTFGLRTAEDLYAAVGAGDVRVSQ
                     VLSGIQALEGDQPQQELLPLDTRRPRINPVTSEFSINGVDNLMTTIAGCCKPVPGDEV
                     "
     gene            complement(1878..>2869)
                     /gene="rlmD"
                     /locus_tag="VFX11_18350"
     CDS             complement(1878..>2869)
                     /gene="rlmD"
                     /locus_tag="VFX11_18350"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX4940641.1"
                     /translation="LQPQELLPPMTGPEFGYRRKARLGAKYVEKKGRALVGFREKSSA
                     FLAEIEQCHVLVPEVGLAFPEFRQLIESLQQRLQIPQIEVAQGDDATALVVRHMQPLP
                     PADLETLVQFCQQRQFHLYLQPAGPDSIHKVWPADNDLRLHYKLEDLGLTLQFHPTDF
                     TQVNPVINRQMVKQAIEFLDPQPGERILDLFCGLGNFTLPLATKSAQVVGVEGDEAMV
                     VRGRENAAANHLTNVEFFGADLTKPFEGQPWAHGGFDKILIDPPRSGALEIVSKMTVF
                     RPKRIVYVSCNPATLARDAGELKKQGYQLLKAGVMDMFPHTTHVESIAVFEPE"
BASE COUNT          525 a          917 c          794 g          633 t
ORIGIN      
        1 acttcgtcgc ccggcaccgg tttgcagcag ccggcgatcg tggtcatcag attgtccaca
       61 ccattgatac tgaattccga tgtgaccgga ttgatgcggg gccgacgggt atccagcggc
      121 agcagttcct gctggggctg atcgccttcc agtgcctgaa tgccgctgag cacctggctc
      181 acccgcacat cgccggcacc gacggccgca tacagatcct cggctgttcg caatccgaag
      241 gtctgcgcca gcggctccag attgatcttg cccacgtcca gccgcttgaa ttccttctcc
      301 agcacttcgc ggccgtcttc gatattgcgg ccacgatcct gctgcttgaa ccagtgcacg
      361 accttggcgc gggctcggct ggtgccgatg aaacccagcg acggttgcag ccagtcacgg
      421 ctcggacgcg cttcgttgcc tttgagaatt tccacctgct cgccggtctg cagcttgtag
      481 ttcagcggca cgatgcggcc attcactttc gcgccccggc agcggtggcc cacttcggta
      541 tgcacgtggt aggcaaaatc cagcggcgtg gcgccggccg ccagatccac cacatggccg
      601 tccggcgtga acacatacac gcgctcgtcg atgatgtctt cgctgaattg cgacagcacg
      661 ctgctgaccg cggaatcgcc cagttcttcc tgccattcca gtacctgccg cagccaggcg
      721 atcttgtcgt cgtagctgtc ggtgcgaccg gtgctgcctt ccttatagcg ccagtgcgcg
      781 cacacgccca gttccgcttc gtcgtgcatg tcctgggtac ggatctgcac ttccagcacc
      841 ttgccttccg gacccagtac cgccgtatgc agcgagcggt aaccgttttc cttcggcgtg
      901 gcaatgtagt cgtcaaattc cttgggaatg tgctgccaca gattgtgcgc aacgcccagc
      961 gcggcataac aatcgcggat ttccggcacc agcacccgca cggcacgaat gtcgtaaacc
     1021 tgatagaaat cgatattctt gcgcttcatt ttgcgccaga tgctgtagat gtgcttggcg
     1081 cggccactga tctcggcttc aatccccacc cgcgccagcg cgtcatgaat ctgcttcacc
     1141 acattgcgga tgtagttgtc gcggtccatg cgcttttcat ccagcagctt ggcgatgcgc
     1201 atgtaggctt cgggttgcag gtaacggaag gacagatcct ccagctccca cttcaagtga
     1261 ccgatgccca gccggtgagc cagcggcgcg tagatatcga aaacctcgcg ggccactttc
     1321 tgctttttgt cttccggcgc ggttttcacc gcgcgaatcg cgcaggtccg ttccgccagc
     1381 ttgatcaggg cgacgcgcac gtcatccacc atcgccacca gcattttgcg caggttgtcg
     1441 agctgatcgg tggcctggcc cagcaccact ttcttctcgg gattcatcac ggcggcaatc
     1501 gccgccatcc gcagcacgcc ttcgatcagt ttggcgatac cggtgcccgc catgcggctg
     1561 acgttttcca gcgtggtctg gcgttcgcgc acggcgcggt aaatcaccgc cgccttcagc
     1621 gaatccgtat ccatgcgcag atcggcgagg atttccacca tttccaggcc ggtcatgaaa
     1681 ctgctggcgg actcggccca ggcattctgg gaattagggg tcttgttttt cagatcgcgg
     1741 ctgtattccg ccagctgcaa cagaccttcc tcgtcggtga tttcgacttt ttctttcagg
     1801 tgctcgatcc acgcccgcag atcgatgctg ccgtcttccc gaaccaccgg attgttgcgt
     1861 actgtcacca ttgatgttta ttccggttca aatactgcaa tggattccac atgggtagtg
     1921 tgcgggaaca tgtccatcac accggccttc aacagttgat agccctgctt tttcaattcg
     1981 cctgcatcgc gcgccagcgt agcgggattg caggatacgt aaacgatgcg tttcggcctg
     2041 aacaccgtca tcttgctgac aatctccagt gcaccggaac gcggcgggtc gatcaggatc
     2101 ttgtcgaaac caccgtgggc ccagggctgg ccctcgaacg gcttggtcag atcggcgccg
     2161 aaaaattcca cgttggtcag atgattggcc gctgcatttt cacgcccgcg caccaccatc
     2221 gcctcatcac cttccacccc caccacctgt gcagacttcg tcgccaatgg cagggtaaag
     2281 ttccccaggc cgcagaacag atccagaatg cgctcgccgg gttgcggatc gagaaattcg
     2341 atcgcctgct tcaccatctg ccgattgatc accggattca cctgggtgaa atctgtggga
     2401 tggaattgca gggtcagacc gagatcctcc agtttgtagt gcaggcgcaa atcgttatca
     2461 gccggccaga ccttgtgaat actgtcaggg ccggcgggct gcagatagag gtgaaactgc
     2521 cgctgctggc aaaactgcac cagcgtctcc agatcggccg gcggcaatgg ctgcatgtgg
     2581 cgcaccacca gcgcggtggc atcgtcgccc tgggccactt caatctgagg aatctgcaaa
     2641 cgctgctgca gggattcgat cagctgccgg aattcgggaa acgccagccc aacttcgggc
     2701 accagcacgt ggcactgttc gatctcggcg agaaaggcgc tgcttttctc gcgaaaaccc
     2761 accagcgcgc ggcccttttt ttccacatat tttgcaccca gacgcgcctt gcgccgataa
     2821 ccgaactccg ggccggtcat gggcggcagc aattcctgcg gctgcaagt
//
LOCUS       DASUEG010000978         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1251598, whole genome shotgun sequence.
ACCESSION   DASUEG010000978 DASUEG010000000
VERSION     DASUEG010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251598"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3340
                     /locus_tag="VFX11_18355"
     CDS             <1..>3340
                     /locus_tag="VFX11_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX4940642.1"
                     /translation="CIDSLQLVNDAELRRLLVLDDGAEAVLPLAPMQRDFCLDSVREP
                     DTQRNSIGAWLALPFAVDAEQWRQALQAVVAANPFLRARFVESSRTWLEPFYQVIARN
                     APAQLGLLDWRDRALPEIELQQALESLALPAWTVLGGPLTRHYLVRVSDQKHIAVVAS
                     HHAVFDGVSKRQYFHQMLDAYAGNSLEAMPVSLLQQWIQQRSDSTDSRAVIDYWRDAL
                     SQSEPLGNRAAQPGAAVVDRWELAADEHALLQSWCRNRGVSVTNYLRTLYALAIRQTD
                     YRDERIVLIDAMAGRDSATGDGDLENAVGCFFQFLPSVQQTGPGIDSIGDLLQANRQW
                     RKRMGDACFLSMLERRQFLNPNALEFQFNYRLPAVTDAFEYQGASLHIEPVQPDNAGT
                     VKLLVTPRNESLHLRLSFRANDFAGFDLLKRMRQVHGQIMAGEENLAQLDWLFAAEKQ
                     QQLQQWQGLPVADYSGETLLDLLARQVDATPDAVAVICGSQQQNYRALDAASNRVAHW
                     LNRQGIGRGQRVAVCLGRGLWLPSLYLGVVKSGAAYVPMDAAYPADRIAYIVEDSQAP
                     VLVTENCVLKRLQEAGVVLPTRTQILLADDIDTLLQDCPDQPLPKLPQPDDILYYVYT
                     SGSTGRPKGAGVYHRGERNLLQWYRQLLQLNGQSRVLLLSALGFDLTQKNLFIPFCTG
                     AALVIPDFEDYDPEQLGHLIAQQNVSVINCAPSAFYPLVEQDSHPGYPFASLRHVVLG
                     GEPIRLGSLKHWFDAPAVNCTLTNSYGPTECTDVVAYCSLQQIDDIHRAMPIGRALPN
                     TQLFIVNQQGALMPTGATGELCVAGIGVGTGYLNRPELNEQSFQPARFGTGAANWYRT
                     GDLVRYNDAGELVYVGRKDFQVKLRGLRIEPGEIDSILKTLAHVRDALTLVVDDRLVS
                     YVIAPRSFDIPAGRDALRKQLPEFMVPGAIVLLDAWPLTPNGKIDRKALPKPDARDQG
                     AHVPPRNDSEQRIADIWCQVLKLSQVSVTANFFEVGGHSLLATQVVSRIRQAFGVELS
                     VRTLFESPTIEKLTHAISSAAAAGFVDTAPPIVPLDPPNRDTLSFAQYRLWFVDQLNQ
                     GSSEYNLPSALKITGPLDVAVLDRV"
BASE COUNT          609 a         1008 c         1051 g          672 t
ORIGIN      
        1 gtgcatcgac agtctgcagc tggtgaatga tgcggaattg cgtcgtctgc tggtgctgga
       61 cgatggcgcc gaagccgtgc tgccgttggc acccatgcag cgggatttct gcctggacag
      121 cgtgcgcgaa cccgataccc agcgcaacag cattggcgcc tggctggcgc tgccgtttgc
      181 ggtggacgcg gagcagtggc gtcaggcatt gcaggccgtt gtcgcagcca atccgtttct
      241 gcgggcacgg tttgtggaaa gcagtcgcac ctggctggaa ccgttctatc aggtaattgc
      301 gcgcaatgcg ccggcacaac tggggttgct ggattggcgc gaccgcgcct tgccggaaat
      361 agaattgcag caggcgctgg aatccctggc gttgccggcc tggaccgtgc tgggcgggcc
      421 gctgacgcgg cattatctgg tgcgcgtgag tgatcagaaa catattgcag tggtggcatc
      481 ccatcacgcg gtgtttgacg gcgtcagcaa gcgccagtat ttccatcaaa tgctggatgc
      541 ctatgccggc aattctctgg aagcaatgcc ggtttcgctg ctgcaacagt ggatacagca
      601 gcgttcggat tccactgaca gccgcgccgt tatcgactac tggcgcgacg cgctgagcca
      661 gtcggaaccg ctgggcaatc gcgccgcgca gcccggtgcc gccgtggtgg atcgctggga
      721 gctggcagca gacgagcatg cgcttctgca gagctggtgt cgcaatcgtg gcgtcagcgt
      781 caccaattat ttgcggaccc tgtatgcgct tgcgatccgg caaaccgatt accgcgatga
      841 acgcatcgta ctgatcgatg cgatggcagg gcgggacagt gcaacaggag atggcgatct
      901 tgagaatgcc gtcggctgtt ttttccagtt tctgcccagc gtgcagcaga ccggtcccgg
      961 tatcgactcc atcggtgacc tgctgcaggc caatcgccag tggcgcaagc ggatgggcga
     1021 tgcctgcttc ctgtcgatgc tggagcggcg ccagtttctg aatcccaatg cgctggaatt
     1081 ccagttcaac tatcgtctgc ctgccgtcac cgacgccttc gaataccagg gtgcgtctct
     1141 gcacatcgag ccggtgcagc cggacaacgc aggcactgtc aagctgttgg tcacgccacg
     1201 caatgaatcc ctgcacttgc ggctgagctt ccgcgccaac gatttcgccg gcttcgatct
     1261 gctgaaacgc atgcgtcaag tgcatggcca gatcatggcg ggcgaggaaa atctggcaca
     1321 gctggactgg ctgtttgccg ctgaaaaaca gcagcaattg cagcagtggc agggcttacc
     1381 ggttgcagac tattccggcg aaaccttgct ggatctgctc gcgcggcagg tggatgccac
     1441 gccggatgcc gtcgcggtca tttgcggcag ccagcaacag aattatcgtg cgctggatgc
     1501 cgccagcaac cgggtggcgc actggttgaa ccggcagggc attggccgtg gccagcgcgt
     1561 agcggtttgt ctgggccgtg ggctgtggct gccatccctg tacctgggtg tggtgaaatc
     1621 cggcgctgcc tatgtgccga tggatgcggc ttatcccgcc gaccgtattg cctacattgt
     1681 ggaagacagt caggcaccgg tgctggtcac cgaaaactgc gtgctgaagc ggctgcagga
     1741 agcgggtgtt gtgctgccca cccgcacaca gattctgctg gcggatgata tcgatacgct
     1801 gctgcaggat tgccccgatc agccgctgcc aaaactgccg cagcccgacg acattctgta
     1861 ttacgtctac acctccggct ccaccggccg gcccaaaggc gcgggcgttt atcatcgtgg
     1921 cgagcgcaat ctgctgcaat ggtaccgcca gctgttgcag ttgaacggcc aatcccgcgt
     1981 gttgctgctg agcgcgctcg gtttcgacct gacccagaaa aatctgttca ttccgttctg
     2041 caccggcgct gcgctggtga ttccggattt cgaggattac gatccggaac agcttgggca
     2101 cctgatcgcg cagcagaacg tttccgtcat caactgcgcg ccgtcggcat tttatccgct
     2161 ggtggaacag gacagtcatc cgggttatcc gtttgcatcg ctgcgccatg tggtgctggg
     2221 tggcgaaccg atccgtctgg gcagcctgaa acactggttc gatgcgcccg ccgtgaactg
     2281 cacactcacc aacagctacg gccccaccga atgcaccgac gtggtggcgt actgctcgct
     2341 gcaacaaatc gacgacattc atcgcgcgat gcccatcggt cgtgcgctgc ccaatacgca
     2401 gttgttcatc gtcaaccagc agggcgcgtt gatgcccacg ggcgccacgg gcgaactgtg
     2461 cgtggccggt attggtgtgg gcaccggtta tctcaaccgc cccgaactga atgaacagtc
     2521 gttccagccg gcgcgctttg gcactggcgc tgccaactgg tatcgcaccg gcgatctggt
     2581 gcgctacaac gatgccggcg aactggtgta tgtgggccgc aaggatttcc aggtgaaact
     2641 gcgcggcctg cgcatcgagc ccggcgaaat cgacagcatc ctgaaaacac tggcgcacgt
     2701 gcgcgatgcg ctgacgctgg tggtggacga ccgtctggtg tcctacgtga tcgcgccgcg
     2761 ttcgttcgac attcctgccg gccgcgatgc actgcgcaaa cagctgccgg aattcatggt
     2821 gcctggtgcg atcgtgttgc tggatgcctg gccgctcacg cccaacggca agatcgaccg
     2881 caaggcgctg cccaagcccg acgcacggga ccagggtgcc catgtgccgc cccgcaacga
     2941 cagcgaacag cgcatcgccg atatctggtg tcaggtgttg aagctgtcgc aggtgagcgt
     3001 caccgccaat ttctttgaag tgggcggaca ttcgctgctg gcgacccagg tggtgtcgcg
     3061 catccggcag gcgtttggtg tggaactgtc ggtacgcacg ctgttcgaat cgcccaccat
     3121 cgaaaaactg acccatgcca tcagcagcgc cgccgccgcc ggctttgtgg ataccgcgcc
     3181 acccatcgtg ccgctggacc cacccaaccg cgacacgctg tcgttcgcac aatatcgcct
     3241 gtggttcgtc gatcagctga accagggcag cagcgaatac aatctgccgt cggcgctgaa
     3301 aatcaccggt ccgctggatg tggcggtact ggaccgcgtg
//
LOCUS       DASUEG010000979         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1254517, whole genome shotgun sequence.
ACCESSION   DASUEG010000979 DASUEG010000000
VERSION     DASUEG010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254517"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFX11_18360"
     CDS             <1..900
                     /locus_tag="VFX11_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HEX4940643.1"
                     /translation="LDLPKSIEAYYQETGRAGRDGEPATALLLYGLEDVVKLGQMMAQ
                     SEGNEQFKRMERQRLDAMLGLCEITSCRRQALLRYFGEEDTQPCGNCDSCLLPPKTWD
                     GTDAVRKLLSCIYRTGQRFGANHVIDVLRGSSSEKILGFEHHKLSTHGIGKEFSATEW
                     KAVVRQLVARGYLAVDADAFGALRLTESCRPLLRGEESIQLRKEVSASQTKATRTPHD
                     DMDEQDVPLWNALRACRKRIAEQLGMPPYVVFHDSTLREMVAQRPVTERALLQISGVG
                     DSKLQKFGDAFLQVIREYEYVEE"
     gene            complement(928..>2343)
                     /locus_tag="VFX11_18365"
     CDS             complement(928..>2343)
                     /locus_tag="VFX11_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940644.1"
                     /translation="WPSSPWLMHLHQLAWFAALQLVVLLFYRRLFPAQALGVLAFALF
                     VLSANFSQTVTWLAARNTLMGATFGIAALLLHIKGWQEDRGLYRGLAVGLFALALAAS
                     EFGLGASTWLFAWVMVMESGSLMRRAGRLLPYGLVMGVWALIYKTGHYGVAQSEFYLD
                     PLQNPAGYVLGLGQRSVDLLFSSLYGFSTGMLGAPTRSLTGWLIACGGVTLVIGLVWN
                     WLQEPHFRFLLLGALLSLLPIAAGPGGARTLAFVSLGMVPFVAALLHRWMSGADRRTL
                     TTGVAWVLVACLALGALARPATAYLMPEYDVTYIQAPAQQLPADDSAPEPQRVILLNP
                     SSVLHAILYPLARVQRALSPGAPTYPLASGVTPIALTRDGSNSLVLSPAEGFLTEPAA
                     FFVRRKDQPLRQGDRFAFSGLQVEILSLNAAQLPHKVRFTFDHPLESPHLQLLQCEHL
                     RFTAFAIPAIGETVVLPACKR"
BASE COUNT          495 a          710 c          771 g          367 t
ORIGIN      
        1 ctggatttgc cgaaaagcat cgaggcctat tatcaggaaa ccggccgtgc cggtcgtgac
       61 ggggagcctg ccaccgcgct gctgctgtac ggcctggagg atgtggtgaa gctgggccag
      121 atgatggcgc agtccgaagg caatgagcaa ttcaagcgca tggagcggca gcggctcgat
      181 gccatgttgg gcctgtgcga aatcaccagc tgccggcgcc aggcgctgct gcgttatttt
      241 ggcgaggaag acacccagcc ctgtggcaac tgcgatagct gtctgttgcc cccgaaaacc
      301 tgggacggca cggatgcggt gcgcaaattg ctgtcctgca tttatcgcac cgggcaacgc
      361 tttggcgcca atcatgtgat cgacgtgctg cgcggcagca gcagtgaaaa aattctcggc
      421 tttgaacacc acaagctttc cacccacggc atcggcaagg aattcagtgc caccgaatgg
      481 aaagcggtgg tgcggcagct ggtggcgcgg ggttatctgg cggtggatgc ggatgcgttt
      541 ggcgcgttgc gtctaactga aagctgccgg ccgttgctgc gcggcgagga atccatccag
      601 ctgcgcaagg aagtgtccgc ctcgcagacc aaggcaacgc gcacgccgca cgacgacatg
      661 gacgaacagg atgttccgct gtggaatgca ctgcgtgcct gtcgcaagcg cattgccgag
      721 caactgggca tgccgcccta tgtggtgttt cacgattcca ccctgcggga aatggtggca
      781 caacgccctg tgaccgaacg cgccctgctg cagatcagcg gtgtgggcga cagcaaactg
      841 cagaaatttg gcgacgcgtt cctgcaggtg atccgcgaat acgagtacgt ggaggaatga
      901 aatccggcgc agccgtcgtt acccgtgtca gcgcttgcag gctggcagca ccaccgtttc
      961 gccaatggcg ggaatggcaa aggcagtgaa gcgcaggtgt tcgcattgca gcaactgcag
     1021 atggggcgat tcaaggggat ggtcaaaggt gaaacgcacc ttgtgcggta gttgcgctgc
     1081 attgagcgac agtatctcca cctgcagacc ggaaaatgca aagcggtcgc cctgacgcag
     1141 gggctggtct ttgcgccgga cgaaaaaggc ggcgggttcc gtcaaaaaac cttcggcggg
     1201 ggacagcacc aggctgttgc tgccgtcgcg ggtgagtgcg atgggcgtta cgcccgatgc
     1261 cagcggatac gtgggcgcgc ccggtgacag cgcccgttgc acccgcgcca gtggatagag
     1321 aattgcgtgc agcacggaac tgggattcag cagaatcacg cgctgcggtt ccggggcgct
     1381 gtcatccgcc ggcagctgtt gcgccggagc ctggatatac gtgacgtcgt attcgggcat
     1441 cagataggcc gtggcaggac gggccagcgc acccagggcc aggcaggcca ccagcaccca
     1501 cgcgaccccg gtcgttaacg tccgccggtc ggcgcctgac atccagcggt gcagcagcgc
     1561 cgccacgaag ggcaccatgc ccaggctgac aaacgccagg gtacgggcgc cgccagggcc
     1621 tgccgcaatc ggcagcagac tcagcagggc gccgagcagc aggaatcgaa aatgcggctc
     1681 ttgcagccag ttccacacca gcccgatcac cagtgtcacc ccgccgcagg cgatcagcca
     1741 tcccgtcagg ctgcgggtag gtgcgcccag catgccggtg gaaaagccgt acagggacga
     1801 aaacagcaga tcaacgctgc gctggcccag tcccagaacg tagccggccg gattctgcag
     1861 cgggtccaga tagaattcgg attgtgccac gccgtagtgg cccgtcttgt agatcagcgc
     1921 ccagacgccc atgacgaggc cgtagggcag cagccgtccg gcacgtcgca tcagactgcc
     1981 ggattccatc accatcaccc acgcaaacag ccaggtgctg gcgcccaggc caaactccga
     2041 cgccgcgagt gccagtgcaa acaggccgac ggccagccca cggtaaagcc cgcggtcttc
     2101 ctgccagcct ttgatgtgca gcagcagtgc ggcaatgcca aaggtcgcgc ccatcagggt
     2161 gttgcgcgcc gcaagccagg tgacggtctg gctgaaattg gccgacagca caaacagcgc
     2221 aaaggccaga acgcccagcg cctgcgccgg aaacagccgt cggtagaaca gcagcacaac
     2281 cagctgcagg gcggcaaacc aggccagctg atgcaggtgc atcagccagg gtgaggaagg
     2341 cca
//
LOCUS       DASUEG010000980         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1818458, whole genome shotgun sequence.
ACCESSION   DASUEG010000980 DASUEG010000000
VERSION     DASUEG010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818458"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFX11_18370"
     CDS             <1..570
                     /locus_tag="VFX11_18370"
                     /inference="COORDINATES: protein motif:HMM:NF016159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEX4940645.1"
                     /translation="DLTHAGFCARINLTHWADNLSEKYLTVSRNRGDRFLQDIFLAWT
                     GFTDTVNVSADTSEFLNIVEAYAQQLPEDTSKEYKSKVVDFCMEQDKNGEPVVYKELS
                     FYLDDAEPERFEKFVQQHQEQPREELIPDKNQLRKYVRFSGRSKEMSLQFAAELLGKQ
                     VEFDASNETLIIRQLPKALLQQLKKHRAS"
     gene            complement(594..1055)
                     /locus_tag="VFX11_18375"
     CDS             complement(594..1055)
                     /locus_tag="VFX11_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940646.1"
                     /translation="MPIRINHLELTLPPGTLEREKANLNGFYRDVLGLEGIEVPMVQL
                     DCPKYLLRFDAESSQFLFLTEEPDALVSTSFDHIGLLLDNRAAVAAAHEKCLAWQQRD
                     ARVEIRPPQDMDAGMLINHSFYVRYILPLWFELQALEYRPGFAPAKAWTYG"
     gene            complement(1154..2794)
                     /locus_tag="VFX11_18380"
     CDS             complement(1154..2794)
                     /locus_tag="VFX11_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4940647.1"
                     /translation="MRTFRDLSFRLKISLPMIIITLMFLASSVSSLLQSRELAANAEQ
                     IVHQHLPEINYILQADRDLYQAQIAERSIIFLPAGSPSLAQYEAQFQENAQQARERVQ
                     KFFDNNSDPRWRSYAERFDTNYRQWLDLASRNVRTRMSGQNIEGDTTTIISKTETSFQ
                     AARAVLDELTDLRIEDANALTDTIESNSASTQRNLLVMLIVGLAVCGAALLFVPPMLV
                     KPLHSIRERLVDISGGSGDLTARIELQQNDEFGQVVQHFNQFMQKLQTLVKQIKDIST
                     EVAGSSTQMNQDAKSSKDSIDQQGDALTLVATAVNQMSAAIQEVARNTTEAADEAKKA
                     SARSEEGQRIVHDTIEQIQQLAAQVQTASSVIVHVEAEAGKVTSVIDVIRGIAEQTNL
                     LALNAAIEAARAGEQGRGFAVVADEVRTLASRTQESTQDIQNMLQHLQQGVKEAVTAM
                     NTSRTSAMTTVQTTEGAGKTLDEIKEAVSNITRMAIQIAAAAEEQSEVTEDINRNLTQ
                     INHFAETTANIATSTLKSSDTLGGLTRTLNQAVQSFRV"
BASE COUNT          599 a          909 c          988 g          745 t
ORIGIN      
        1 gacctgaccc acgccggatt ttgcgcgcgc atcaatctga cccactgggc tgacaacctg
       61 tcggaaaaat acctcacggt gtcacgcaat cgcggcgacc gtttcctgca ggatattttc
      121 ctggcctgga ccggttttac cgacacagta aacgtgtccg ctgacaccag cgaatttctc
      181 aatatcgtgg aagcctatgc ccagcagttg ccggaggaca ccagcaagga atacaagagc
      241 aaggtggtgg atttctgcat ggagcaggat aaaaacggcg agccggtggt gtacaaggag
      301 ttatcgttct atctggatga cgccgagccg gagcggttcg agaaattcgt gcagcagcat
      361 caggaacagc cgcgcgagga actgattccg gataaaaacc agctgcgcaa atacgtgcgc
      421 ttcagcggtc gcagcaagga aatgagtctg cagttcgcgg cggaattgct gggcaagcag
      481 gtggagttcg atgcgtcgaa cgagacgctg attatccgcc agttgcccaa agcgttactg
      541 cagcagctga agaaacaccg cgcgtcctga aaaacagcgt gcgttcgcgc ctgtcagccg
      601 taagtccagg ctttggcagg tgcaaacccc ggccggtact ccagcgcctg cagttcgaac
      661 cagagtggca ggatgtagcg cacgtaaaag gaatgattga tcagcatgcc ggcgtccatg
      721 tcctggggcg gccggatttc cacccgcgca tcccgctgct gccacgccag gcatttttcg
      781 tgggccgccg ccacagcagc gcggttatcc agcagcagcc cgatgtggtc gaaactggtg
      841 gacaccagcg cgtcgggctc ctccgtcaga aacaggaact ggctggattc cgcgtcgaac
      901 cgcagcaggt atttggggca gtccagctgc accatcggta cctcgatccc ctccagcccc
      961 agcacgtcgc gatagaaccc gttgagattg gccttttccc gttccagggt gccgggcggc
     1021 agggtgagtt ccagatgatt gatgcgaatg ggcatgacgt gatctccagg cagcgtatgg
     1081 ctgcatgaca tcacggtagc ccgtcgccgg catcgtccgt tggaacgatt gtgctcgccg
     1141 acgggctggc ggtttatacc cggaagctct gcaccgcctg gttcagggtg cgggtcaggc
     1201 cacccagcgt gtcgctggat ttcagcgtgg acgttgcgat gtttgccgtg gtttcggcga
     1261 agtgattgat ctgggtgaga ttgcggttga tatcctccgt cacctcgctc tgctcttcgg
     1321 ccgcggccgc gatctggatg gccatgcggg taatgttgga tacggcttcc ttgatctcat
     1381 ccagcgtctt gccggcgcct tcggtggtct gcaccgtggt catcgccgat gtgcggctgg
     1441 tgttcattgc cgtcaccgct tccttcacgc cctgctgcag atgctgcagc atgttctgga
     1501 tatcctgggt ggattcctgg gtgcggcttg ccagcgtgcg cacttcatcg gccaccaccg
     1561 caaagccacg gccctgttcg ccggcgcgtg ccgcttcaat cgccgcgttc agtgccagca
     1621 ggttggtctg ctcggcgatg ccgcgaatca cgtcgatcac cgatgtcacc ttgccggctt
     1681 ccgcttccac atgcacgatc acgctggagg cagtctgcac ctgcgctgcc agttgctgga
     1741 tctgctcgat ggtgtcgtgc acgatgcgct ggccttcctc ggaacgggcg ctggcctttt
     1801 tcgcttcgtc cgctgcttcg gtggtgttgc gcgccacttc ctgaatcgcg gcggacatct
     1861 ggttcaccgc cgtggccacc agcgtcagcg catcgccctg ctgatcgatc gaatccttgc
     1921 tggactttgc gtcctgattc atctgcgtgc tgctgccagc cacctccgtt gaaatgtcct
     1981 tgatctgttt caccagcgtc tgcagtttct gcatgaactg gttgaagtgc tgcaccacct
     2041 ggccgaactc gtcattttgc tgcagctcga tgcgggcggt gagatcgccg gaaccgccgg
     2101 aaatgtccac caggcgctca cggatcgaat gcagcggttt caccagcatg ggcggcacga
     2161 acagcaacgc ggcgccgcac accgccagcc cgacgatcag catgaccagc agattgcgct
     2221 gggtgctggc ggaattgctt tcgatggtgt cggtcaatgc attggcatct tcaatgcgca
     2281 ggtcggtcag ttcgtccagc acggcacggg cggcctggaa cgaggtttcg gttttgctga
     2341 tgatcgtggt ggtgtcgcct tcgatgttct ggccgctcat gcgggtgcgc acgttgcggc
     2401 tggccaggtc gagccactgc cggtaattgg tgtcaaagcg ttcggcgtag gaccgccaac
     2461 gcggatcgga attgttgtcg aaaaatttct gtacgcgctc gcgcgcctgt tgcgcgttct
     2521 cctggaactg cgcttcatat tgcgcgagac tgggcgagcc ggcgggcagg aagatgatgc
     2581 tgcgctcggc gatctgcgcc tgatacaagt cgcgatcggc ttgcaggatg taattgattt
     2641 ccggcaggtg ctgatgaacg atctgttccg cgtttgccgc cagttcgcgc gactgcagca
     2701 ggcttgacac ggaagaggcc agaaacatga gcgtgatgat gatcatgggc aggctgatct
     2761 tgaggcggaa ggaaagatct cggaaggtgc gcataggtgt cctgtcgtta tttcagttat
     2821 tccagttccc tgatttattt ctagtccaaa aaacaggcag atcaaactgt taacggtgat
     2881 cgtaagtggc attcatcgcc atttgttcta acaaatgggc ggatttgcgc acactcacgc
     2941 aggatttctg tttcatcttt tttaaaatca gatgcttgca ctcctaatct ttatcggttg
     3001 ccattcgttg catttcgtaa catgacactg tcaaattacg ctgtgtagac tagcggcgct
     3061 ggtcaggtgc gggggcgtct gaccggtttg ggggcaatcg cgcgaccggt ctttgtcgct
     3121 ggcgcaaggt tgcagttgtg caggtgaagc cgcaggtatt gcggcccctg cgtagtcggt
     3181 aatggaaggt cagataccat gtggaagtgg agcttcttgc gttcatcgtt cgttgctacc
     3241 g
//
LOCUS       DASUEG010000981         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1295829, whole genome shotgun sequence.
ACCESSION   DASUEG010000981 DASUEG010000000
VERSION     DASUEG010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295829"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFX11_18385"
     CDS             complement(<1..241)
                     /locus_tag="VFX11_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007242311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX4940648.1"
                     /translation="MVSDDSSNDNKVRLDKWLWAARFYKTRSIAKEMIEGGKVHYDGH
                     RVKASREVALGATIRLRQGVDEKEVVVTGLETRRGN"
     gene            380..2230
                     /locus_tag="VFX11_18390"
     CDS             380..2230
                     /locus_tag="VFX11_18390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940649.1"
                     /translation="MKPWWTLWLLVLLTPVQAEALRLQPGQERYAVGPFLQVWEDVSA
                     RLGPAEVLALREQFEPVQDENVTQGYSHSAWWFYLALDEAGAAGEWLLHVDYSALDYL
                     DVWQVSGAAEPRSLYSGGDHLPFPRRPLLHSAFLVPFVLQPQEHSALLIRVQTQGSVI
                     MPLQILSKNAFIEDEESARLMLGGLWGGLLFLMLYNLLLYRNVRDRVYLYYCAYILAF
                     VVGSLHLSGVGFHYLWPNWLWMQTGGLPVLFLLASFFANNFSRLLLDLKRRHPLLDRI
                     LYGFAVLHVICMLVFPLLPFQLSLYCTVAVVLSMGVFLMTAGVMSFVENYPPGRNYFL
                     AWISLFIAVVIFALETMGLTHGSFISKWSLQIGAGVEALLFAIAMSDRINLFKQQALQ
                     AQEKVVEQEKLLRIAQEEANRELEQRVDQRTRELAKVVEDLARVNEKLDRLNQLDELT
                     HVFNRRAFNHRSATLLAQCKREQRSLAVLLLDIDHFKQVNDRYGHLAGDECLRRIAAL
                     IQARVTRPQDFVARLGGEEFVVVLYDTPADGALHVAERIRVEVQQACFPWDDQCIPLT
                     VSVGLCCGVPASWHEVEHFTGWADQALYQAKSAGRNRAVHRDLVTVAECN"
     gene            complement(2360..3310)
                     /locus_tag="VFX11_18395"
     CDS             complement(2360..3310)
                     /locus_tag="VFX11_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017043660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX4940650.1"
                     /translation="MLPFHTPRISRLWLPGLFLLCNFCVSTTANAATDGYTRTRYPIV
                     LIHGMALFDDMLGVSGWYSIPRQLRKGGANVFVLQVSALNTTELRGEQAARQIETILA
                     TTGAEKVNLIGHSHGSPTARYLAGVYPHYVASVTSVHGVNWGTPVADAMQQDLAERNL
                     SGRLFKGVMSAWGALVNVLSGDPLPQSYPLEIEALTTAKALAFNAQYPDGMPQQYCGD
                     GAPVVNGIHYFSWTGNRVMTNLADLADYGLLASAALMDEPNDGLVPVCATYLGQVIRD
                     NYRMNHIDAIDHLFGLHSSKDTDPPVVYRQQANRLQQLGL"
     gene            3533..>3862
                     /locus_tag="VFX11_18400"
     CDS             3533..>3862
                     /locus_tag="VFX11_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940651.1"
                     /translation="MKKNGLLTLAFGVAVLAVCAWVLVGPFLVKPGLSVPGVQMLPAP
                     AALPGVATSPGITTSAEFLRWAQQGAALGDVPRSLQGTAVDGGVELDAQGNLVVGESL
                     RRLFDYFL"
BASE COUNT          652 a         1044 c         1323 g          843 t
ORIGIN      
        1 cattgccgcg ccgggtttcg agtccggtca ccacgacttc cttttcatcc acaccctggc
       61 gcaggcggat ggtggcgccc agggccactt cccggctggc tttgacacgg tgaccgtcgt
      121 agtgcacttt gcctccttcg atcatctcct ttgcgatgga ccgggtcttg tagaagcgtg
      181 ccgcccacag ccatttgtcg agtcgcacct tgttgtcgtt gctgctgtcg tcggaaacca
      241 tgagtattgc gtcttcgcgc gggtggtggg gctggtcagg ggtggtcatt ataaaaggat
      301 tttgcaccgg agtatgcccg cgtcatgtgg atgacgacgc actaaacagg aaacgcagga
      361 atttgagggc caggttcaaa tgaaaccatg gtggacattg tggctgctgg tgctgctgac
      421 gccggtgcag gccgaggcgc tgcggttgca gccaggacag gagcggtatg ccgtgggccc
      481 gttcctgcag gtgtgggagg atgtgtcggc ccgtctgggc ccggcggagg tgctggcgct
      541 gcgggagcag ttcgagccgg tgcaggatga aaatgtgacg cagggctaca gccactcggc
      601 ctggtggttc tatctggcgc tggatgaggc gggggcggcg ggggagtggc tgctgcatgt
      661 ggattacagt gcgctggatt atcttgatgt gtggcaggtg agtggtgcgg cagagccccg
      721 ctccctgtac agcggtggcg atcatctgcc ctttccccgg cgaccgttgc tgcacagcgc
      781 attcctggtg ccctttgtgc tgcagccgca ggaacattca gccctgctga tccgggtgca
      841 gacccagggc tccgtcatca tgccactgca gatcctgtcg aaaaacgcct tcattgagga
      901 tgaggaaagc gcgcgcctga tgctgggcgg gctatggggc gggctgctgt tcctgatgtt
      961 gtacaacctg ctgctgtacc gcaacgtgcg tgaccgcgtc tatctgtatt actgcgccta
     1021 tatcctggcc ttcgtggtgg gctcgctgca cctgtccggc gtcggcttcc attatctgtg
     1081 gccgaactgg ctgtggatgc agaccggtgg tctgccggtg ctgttcctgc tggcatcctt
     1141 cttcgccaac aatttctcac gcctgctgct ggatctgaag cgccggcatc cgctgctgga
     1201 ccggattctg tacggctttg cggtgctgca tgtgatctgc atgctggtgt ttcccctgct
     1261 gccgttccag ctgtcgctgt attgcaccgt tgcggtcgtg ctcagcatgg gcgttttcct
     1321 gatgacggcg ggtgtgatgt cgttcgtgga aaactatccg ccggggcgga attattttct
     1381 ggcctggatc agcctgttca tcgcggtagt gattttcgcg ctggaaacca tggggctcac
     1441 ccacggatcg ttcatctcga aatggtcgct gcagattggc gcgggtgtgg aggcgctgct
     1501 gtttgccatc gccatgtcgg atcgcatcaa tctgttcaaa cagcaggcgc tgcaggcgca
     1561 ggaaaaagtg gtggagcagg agaagctgct gcggatcgcg caggaggaag ccaaccgcga
     1621 gctggaacaa cgggtggacc agcgcacccg cgagctggcg aaagtggtgg aagatctggc
     1681 gcgggtgaac gagaagctgg atcgcctgaa ccagctggac gaactgaccc atgtgttcaa
     1741 ccggcgcgcc ttcaatcacc gcagtgccac cctgctggcc cagtgcaagc gggagcagcg
     1801 cagcctggcg gtattgctgc tggatatcga tcacttcaag caggtgaacg accgctacgg
     1861 ccatctggcg ggggacgaat gtctgcgccg gatcgcggca ctgatccagg cgcgggtgac
     1921 gcggccgcag gatttcgtgg cgcggctggg tggcgaggaa ttcgtggtgg tgctgtatga
     1981 cacgccggcg gatggtgcgc ttcatgtggc ggagcgcatt cgcgtcgagg tgcagcaagc
     2041 ctgcttcccg tgggacgacc agtgtattcc gctgacggtg agcgtgggcc tgtgctgcgg
     2101 tgttcctgcc agctggcatg aggtggagca tttcaccggc tgggcggatc aggcgctgta
     2161 ccaggccaag tccgctggcc gcaaccgggc ggtgcatcgg gatctggtga cggtggcgga
     2221 atgcaattga actgaaaaag acaccattca ccggggctcg ctgaccagtg aatggtgttt
     2281 tgcagtgaca acaggcgtgc agttacaacc tggtgcagtt acaagagtgg cgctagttct
     2341 tgtggtaagt gctggaaaat cagaggccca gttgttgcag ccggttggcc tgctggcggt
     2401 ataccaccgg tggatcggtg tccttgctgc tgtgcaggcc gaacaggtgg tcgatggcat
     2461 cgatatgatt catgcggtag ttgtcgcgga tcacctgtcc cagataggtg gcgcacaccg
     2521 gcaccagacc gtcgttgggt tcgtccatca gcgccgctga cgccagcagg ccgtagtcgg
     2581 cgaggtccgc cagattggtc atgacgcggt tgccggtcca ggaaaaataa tggattccgt
     2641 tgaccaccgg cgcgccgtca ccgcaatact gttgtggcat gccatccgga tattgtgcgt
     2701 tgaatgccag cgccttggcc gtggtaagtg cctcgatttc cagcggataa ctttgtggca
     2761 aaggatcgcc gctcagcaca ttcaccagcg cgccccaggc gctcatcacc cctttgaaca
     2821 gtcggccgga gagattgcgc tccgccagat cctgctgcat ggcatcggcc accggtgtac
     2881 cccagttcac gccgtgcacg ctggtcaccg aggcgacgta gtgcggatac accccggcca
     2941 gatagcgcgc ggtggggctg ccgtgactgt ggccgatcag gttcactttt tccgccccgg
     3001 tggtggccag gatggtttcg atctggcggg ccgcctgctc gccacgcagt tcggtggtat
     3061 tgagcgccga tacttgcagc acgaacacat tggcgccacc cttgcgtaac tggcgcggaa
     3121 tgctgtacca gccgctgacc cccagcatgt cgtcgaacag ggccatgccg tgaatcagaa
     3181 cgatgggata gcgggtgcgg gtgtaaccgt cagtagctgc gtttgcggtt gtgctgacac
     3241 agaaattgca cagcaagaac agtcccggta accacaggcg ggaaatgcgc ggtgtgtgga
     3301 agggcagcat gagagaatcc ttgttttatt attgtccggg aacgttgctg gccggtgaat
     3361 ctggcattcc gtctggcagg ctagccggtt gcggggtttt ccgacatgga cttgatccgg
     3421 tttggccatt tgcgcgggcg gaaatggccg ggtcggacag ctgctgtgca taaactatgc
     3481 gatggctgtc cactggttac ggacagggaa tgcctgcaag gagagggtcg ccatgaaaaa
     3541 gaacgggttg ttgacactgg ccttcggggt tgcggtgctg gcggtgtgcg cgtgggttct
     3601 ggtgggtccc tttctggtga agccggggct gtcggtcccc ggcgtgcaga tgctgcctgc
     3661 gccggcggcg ctgccagggg tggcaacgtc acccgggatc acgacgtcag ctgaatttct
     3721 gcggtgggcg caacagggcg cggcactggg tgacgtgccg cgttcgctgc agggcacggc
     3781 agtggatggc ggcgtggagc tggacgcgca gggcaatctg gttgtggggg aatcattgcg
     3841 gcgcctgttc gattattttc tt
//
LOCUS       DASUEG010000982         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1582819, whole genome shotgun sequence.
ACCESSION   DASUEG010000982 DASUEG010000000
VERSION     DASUEG010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582819"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFX11_18405"
     CDS             complement(<1..333)
                     /locus_tag="VFX11_18405"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEX4940652.1"
                     /translation="MQWLLDVITLKFFITPYLILLIYWFGAFVVPVAGWLLCWWLLTR
                     LKENEIASAGLDGLGNGIGRLPRSGQFKRYFWIAFTLVFLFMELLWRLLFEFLIAYFH
                     IHDALMQLS"
     gene            complement(358..765)
                     /locus_tag="VFX11_18410"
     CDS             complement(358..765)
                     /locus_tag="VFX11_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940653.1"
                     /translation="MSFKPLILALLVGTSLLLQGCISFNSLADARDGKGSGQVKEYSA
                     NRDQVWQQALAIIEESDLNLVNADATQGLILAQQPIAPLSLTAGQNVAIYVSESHGRT
                     RVEVISRKALGDIEFVSKDWEDYILERLDQRFI"
     gene            complement(814..2766)
                     /locus_tag="VFX11_18415"
     CDS             complement(814..2766)
                     /locus_tag="VFX11_18415"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940654.1"
                     /translation="MASLVTGGTGFIGKRLIDRLLDRNESVFVLVRDRSLPALQHVIA
                     TRWQQKADLIQPLTGDICQPLAGISADQLETLKSQVERVYHLAALYDLNMSDAEADAI
                     NITGTRNVVQLSNALGCPLHHVSSIAVTGGQYRGRFTEAMFDEGQSLQHPYYRTKFDS
                     EALVRAEAQVPFRIYRPGIVVGDSRTGEMDKLDGPYYLFPLLLMLRSLPRQLPLIGLD
                     AGRMNLVPVDYVASALDAISHQDDLSGNCFHLVAKRDLKVCEVMNLFARSAGAPTFAA
                     LLPERLSRLLLRGVGASLRHSRYGQVTKQTFFEKTRMPASALDVLNWHTTFDARATEA
                     VLQPLGITCPDPTDYADTLWRYWQQHLAPKRQHKNLHHKKPRWTPEIKGKRVLITGAS
                     SGIGKALALQLASKGATVLLVARSAEKLEDVRREIRLAGGSAFAYPCDLTVEEDVKKL
                     ITQLDAEPPIDVLVNNAGRSIRRSLSHSCDRLHDFERTMQLNYFGALRITLGLLPRMR
                     QERNGHIIHVSSVGVPVHSPRFAAYLASKAAFDEFSRVAAGELRQDNIHFSIIYMPLV
                     RTSMIAPTESYKHTPALSPESAANLIVRTIQRRPARVMNGFSRSMQLLHGLTPKLGVR
                     ALNLGYRYTSGTAGRPAQGNAASDQT"
     gene            complement(3045..3302)
                     /locus_tag="VFX11_18420"
     CDS             complement(3045..3302)
                     /locus_tag="VFX11_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX4940655.1"
                     /translation="MSPIAVLIACWLGLVLLSVTTVLLGSSGSSGLLTAGILLAALGK
                     GWIIIDRFMELRHTRSFWRPLLLAWPVLMAVVVGFGLLMRP"
     gene            complement(3332..3859)
                     /locus_tag="VFX11_18425"
     CDS             complement(3332..3859)
                     /locus_tag="VFX11_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4940656.1"
                     /translation="MWFFILAELTVFGILIPAFVVTERLHPELFHASRALLDTSTGLV
                     LTLSLLTAGLLAALAVEQVRHSRHRTGTGLLAGAQLSASIYVIIKFNEYRHLASLGLD
                     IEYDAFFRLYWLLTGFHFLHVLLGLVILAFMTFRCWRQAYGPGQSSSLESGVLYWHMV
                     DLVWVMLFPLVYVIN"
     gene            complement(3969..>4258)
                     /locus_tag="VFX11_18430"
     CDS             complement(3969..>4258)
                     /locus_tag="VFX11_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4940657.1"
                     /translation="IEQINRTGFGLTLGVHSRINRFCESVTKKAQVGNVYINRNMIGA
                     TVGSQPFGGQDLSGTGPKAGGPHYLLRLANEKTVSINTAAIGGNTRLLTED"
BASE COUNT          811 a         1247 c         1395 g          805 t
ORIGIN      
        1 tgacagctgc atcagcgcat cgtggatgtg gaagtaggcg atcaggaatt caaacagcag
       61 gcgccacaac agttccatga acaggaacac cagggtgaag gcgatccaga aatagcgctt
      121 gaactggccc gagcggggca gccggccaat gccgttgccc aggccgtcca ggccggcgga
      181 ggcgatctcg ttttccttca gcctggtcag cagccaccag cacagcagcc agcccgccac
      241 cggcaccacg aaggcgccga accagtagat gagcaggatc aggtagggcg tgatgaaaaa
      301 tttcagggtg atgacatcca gcagccattg catgggttga gaactccggt gatcgtgtca
      361 gatgaaacgt tggtccagcc gttcgagaat gtagtcttcc cagtctttcg acacgaattc
      421 gatatccccc agcgccttgc ggctgatcac ctccacccgg gtgcggccgt ggctttcgct
      481 gacgtagatg gccacgttct ggccggcggt caggctcagc ggtgcgatcg gctgttgcgc
      541 caggatcagg ccctgggtgg cgtcggcatt caccagattc aggtccgatt cctcgatgat
      601 ggcgagcgcc tgttgccaca cctgatcgcg gttggcgctg tactccttca cttgtccgct
      661 gcccttgccg tcgcgggcgt ccgccaggct gttgaacgag atgcagccct gcaataacag
      721 gctggtgccc accagcaggg cgaggatgag aggtttgaat gacatggtga tctcctttgt
      781 gtttgttggt ggtggaattg aagtgaatgc ggttcaggtc tggtcggacg cggcattgcc
      841 ctgcgccggt ctgccggcgg tgccgctggt gtagcggtaa cccaggttca gcgcgcgtac
      901 gcccagtttg ggtgtgagcc cgtgcagcaa ttgcatgctg cgggaaaatc cgttcatcac
      961 ccgtgccggc cggcgctgga tcgtgcgcac aatcaggttg gcggcggact ccggcgacag
     1021 cgcaggcgtg tgcttgtagc tttccgtcgg cgcgatcatc gaggtgcgca ccagcggcat
     1081 gtagatgatg gaaaaatgaa tgttgtcctg acgcaattcc ccggcggcga cacggctgaa
     1141 ttcgtcgaag gccgccttgc tggcgaggta ggcggcaaag cgtggcgaat gcaccggcac
     1201 gccaacgctg gagacgtgga tgatgtggcc gttgcgttcc tgccgcatgc gtggcagcaa
     1261 acccagggtg atgcgcagcg caccgaaata attcagctgc atggtgcgtt cgaaatcgtg
     1321 caggcggtcg caggaatggg acagcgagcg gcggatgctg cggccggcgt tgttcaccag
     1381 cacgtcgatg ggtggttctg catccagttg cgtgatgagt tttttgacat cttcttccac
     1441 cgtcagatcg caggggtagg caaacgcgct gccgccggcc agacggattt cgcggcgcac
     1501 gtcctccagc ttttctgccg agcgggccac cagcaacacc gtggcgccct tgcttgccag
     1561 ttgcagcgcc agcgccttgc caatgccgct gctggcgccg gtgatcagta cgcgcttgcc
     1621 tttaatttcc ggcgtccagc gcggtttttt gtggtgcagg tttttgtgct ggcgcttggg
     1681 cgcaagatgc tgttgccagt agcgccacag ggtgtcggca taatccgtcg ggtccgggca
     1741 ggtgattccc agtggttgca gaacagcttc ggttgcgcgc gcatcgaacg tggtgtgcca
     1801 gttgagtaca tcgagggccg aggcgggcat gcgggttttt tcgaagaacg tctgcttagt
     1861 cacctggcca tagcgggagt ggcgcaggct ggcgccgaca ccccgcagca gcagacgcga
     1921 cagacgctcc ggcaacagcg cggcgaaggt gggagcgccg gcggagcggg cgaacaggtt
     1981 catcacctcg cagactttca gatcgcgctt tgccaccagg tggaagcagt tgccggacag
     2041 gtcatcctgg tgggaaatgg cgtccagcgc actggccacg taatccaccg gcaccaggtt
     2101 catccgtccg gcgtccagcc cgatcagcgg cagctggcgc ggcagcgacc gcagcatcag
     2161 cagcagcggg aacaggtaat aggggccgtc cagcttgtcc atctcgcccg tgcgcgaatc
     2221 ccccaccacg atgcctgggc ggtaaatgcg gaacggcacc tgcgcttccg cccgcaccag
     2281 cgcctcggaa tcgaacttgg tgcggtagta gggatgctgc agggactggc cttcgtcgaa
     2341 catggcctcg gtgaagcggc cccggtactg gccgccggtg accgcgatgg aactcacatg
     2401 atgcagcggg cagcccaggg cgttgctcag ttgcaccacg ttgcgggtgc cggtgatgtt
     2461 gatggcatcg gcctcggcgt cactcatgtt gagatcgtag agcgccgcca gatggtagac
     2521 gcgctccacc tggcttttca gcgtttccag ctggtccgca ctgatgcccg ccagtggctg
     2581 gcagatatcg ccggtcagcg gctggatcag gtcggccttc tgctgccagc gggtggcgat
     2641 cacgtgctgc agggccggca gggaccggtc ccgtaccagc acaaaaacag attcattccg
     2701 gtccagcagc cggtcgatca ggcgtttgcc gataaacccc gtgcccccag tgactagcga
     2761 cgccatgcgg cggtcctcct gcttgatgac ggttggatcg ggtgtcggac gagcctgttc
     2821 aagggccatc cgtcacaagt tactcattgg taatatgtgt aacttaccag tcggtaactt
     2881 aaagtcaagg gggagttttg acctgtgccg tcgtggggga gcatcgggcg ggtgggattt
     2941 gcaaggggga aaggttgaag gccggggctg ttgtcggtag tggctcgacg tggaatggcc
     3001 tgatctggat tgagtgggtt tggggctgcg gttgtcgtgc ctggttacgg ccgcatcagc
     3061 aggccgaagc cgacgacgac ggccatgagc accggccagg ccagcagcag cggacgccag
     3121 aaactgcggg tgtggcgcag ttccatgaag cggtcaatga tgatccagcc cttgcccagc
     3181 gcggccagca aaatgccggc ggtgagcagg ccactgctgc cgctgctgcc cagcagaacg
     3241 gtggtcaccg acagcagcac cagtcccagc cagcaggcga tcaggactgc gatgggcgac
     3301 atgcgcaaat ttcccaagcg tgaaaactcc atcagttgat cacatacacc agcggaaaca
     3361 gcatcaccca caccagatcc accatgtgcc agtacagcac gcccgattcc aggctgctgc
     3421 tctggccggg accataggcc tggcgccagc atcggaacgt catgaaggcc aggatcacca
     3481 gccccagcag cacgtgcaga aaatgaaagc cggtgagcag ccagtacagc cggaagaagg
     3541 catcgtattc gatatccagc cccaggctgg ccagatgccg gtattcgttg aacttgatga
     3601 tgacgtaaat gctggccgac agttgcgccc ccgccagcag ccctgtgccg gtgcgatgac
     3661 gggaatggcg cacctgttcg acggcaagcg ccgccagcag tccggcggtc agcaggctga
     3721 gggtgaggac caggccggtg gaggtatcga gcagcgcccg gctggcatgg aacagttccg
     3781 ggtgcagccg ttcggtgacg acgaaggcgg ggatcagaat gccgaacacc gtcagttccg
     3841 ccagaatgaa aaaccacatc gccagatcgc ccggcagtgc gtgtggtttt gatgcagcag
     3901 aaaaggcagt ggactcgttc aaggcattcc cggccggtaa gaaaaattcc ggcttcgata
     3961 gtgccacctc aatcctcggt cagcaaccgc gtgttgccgc cgatggccgc cgtgttgatc
     4021 gacacggttt tttcattggc caatcgcagc aaataatgcg gcccgcccgc tttcggcccc
     4081 gtgcccgaca gatcctgccc gccaaacggc tgcgatccca cggtggcgcc gatcatgttg
     4141 cggttgatgt acacgttgcc cacctgcgcc ttcttcgtca cgctttcgca gaagcggttg
     4201 atgcggctgt gcacgcccag ggtgaggccg aagccggtgc ggttgatctg ctcgatca
//
LOCUS       DASUEG010000983         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1261675, whole genome shotgun sequence.
ACCESSION   DASUEG010000983 DASUEG010000000
VERSION     DASUEG010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261675"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFX11_18435"
     CDS             <1..578
                     /locus_tag="VFX11_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4940658.1"
                     /translation="RPIQQITGTCEFNEVFFDDAECAADDIVGKPGDGWKVAMGLLGF
                     ERGVSTLGQQMQFQNELNEIIAVARQNGADKNPVLRQRIAKAHAELKIMRYNSLRMLS
                     GQSGSDGSLQKEALIYKLFWATWHRDLGELAMDVMGPESQILDAEPYQLNRLQALYLF
                     TRSDTIYGGTNQIQRNIIAERGLGMPKEPRG"
     gene            646..1437
                     /locus_tag="VFX11_18440"
     CDS             646..1437
                     /locus_tag="VFX11_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940659.1"
                     /translation="MDIHNPTYVPGHGLLKGKSVLITAAAGAGIGFAAAKKCVEEGCR
                     GIVISDIHAGRLEQSVQKLKEETGFNNVFGQIGNVAVEDDVQALINFAEDKLQGVDVL
                     INNAGLGTTRALIDMEDSEWNKVIDVTLNGTMRMTRYMLKIMKARGNGGVIVNNASVL
                     GWRAQKEQSHYAAAKAGVMALTRCAALEAAEFGVRINAVSPSLAVHPMLKKSAPAELL
                     AELEGREAYGRGAEVWEVANIMVFLASDYSSYLTGEIISCSSQRA"
     gene            1450..1908
                     /locus_tag="VFX11_18445"
     CDS             1450..1908
                     /locus_tag="VFX11_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4940660.1"
                     /translation="MTTVFKSPLDLEAAVGKRLGESDWLTIDQARINLFADATGDHQW
                     IHVDPEKAKTGSFGTTIAHGYLTLSLVNMFLPQIVDVQGISMGVNYGADKLRFPSPVK
                     VNSRVRGVGELVAVEQVKGGVQATIRVTVEIEGSDKPACVVDTISRYYPK"
BASE COUNT          469 a          566 c          628 g          386 t
ORIGIN      
        1 tgcggccgat ccagcagatc acgggcacct gcgaattcaa cgaagtgttt ttcgatgatg
       61 ccgagtgcgc ggccgatgat atcgtcggca aacccggtga tggatggaaa gtggcgatgg
      121 gtctgctggg tttcgagcgc ggcgtttcca ccctcggcca gcagatgcag ttccagaacg
      181 aactgaacga gatcatcgcc gttgccaggc agaatggtgc cgataaaaat cccgtactgc
      241 gccagcgcat tgcgaaagcc catgctgaac tgaagatcat gcgctacaac tcgttgcgca
      301 tgctgtccgg ccagtctggc agtgacggct cgctgcagaa ggaagccctg atctacaaac
      361 tgttctgggc cacctggcac cgcgatctgg gcgaactggc gatggatgtg atgggcccgg
      421 aaagccagat cctggacgca gagccctatc agctgaaccg gttgcaggcg ctgtatctgt
      481 tcacccgctc cgacaccatt tacggcggca ccaaccagat tcagcgcaac atcatagccg
      541 agcgcggcct gggcatgccc aaagaaccac gcggatagat aaagaggagg gctcggtctg
      601 gtactaccag gccaagtgct ccgacacaca ttaagagggt tcaccatgga cattcacaat
      661 cccacctacg tgcccggcca cggcctgttg aaaggaaaat ccgtgctgat cactgctgcg
      721 gccggtgccg gcatcggttt cgcggcggcg aaaaaatgtg tcgaggaagg ttgtcgcggc
      781 attgtgatca gcgacattca tgccggccgt ctggaacagt cggtgcagaa gctgaaggaa
      841 gaaaccggct tcaacaacgt attcggccag atcggcaacg tcgcagtgga agacgacgtg
      901 caggcgctga tcaatttcgc cgaagacaaa ttgcagggtg tcgatgtgct gatcaacaac
      961 gctggcctgg gcaccaccag ggcgctgatc gacatggagg acagcgagtg gaataaagtc
     1021 atcgatgtga cgctcaacgg caccatgcgc atgacccgct acatgctgaa aatcatgaaa
     1081 gcgcgcggca acggcggtgt gatcgtcaac aacgcctcag tgctgggatg gcgtgcgcag
     1141 aaggagcagt cgcactatgc cgctgccaag gcaggcgtga tggcactgac ccgttgcgcg
     1201 gcgctggaag cggcggagtt cggtgtgcgc atcaatgcgg tgtcgccgtc gctggcggtg
     1261 catccgatgc tgaagaaatc cgcgccggcg gaactgctgg cggaactgga aggccgcgaa
     1321 gcctacggcc gtggtgccga ggtgtgggaa gtggcgaaca tcatggtgtt tctggcgtcg
     1381 gattattcca gctatctgac cggcgaaatc atttcctgtt ccagtcagcg tgcgtaaggg
     1441 gattgcagca tgacaaccgt attcaaatca ccgctggatc tggaagccgc agtaggcaag
     1501 cgcctgggtg agagtgactg gctcaccatc gaccaggccc gcatcaacct gtttgccgat
     1561 gccactggtg atcaccagtg gattcatgtt gatccggaaa aagccaaaac cggcagcttc
     1621 ggtaccacca ttgcccacgg ctatctcacg ctgtcactgg taaatatgtt cctgccacag
     1681 atagtcgacg tgcagggtat cagcatgggc gtgaattacg gtgccgacaa attgcggttt
     1741 ccgtcgccgg tcaaggtgaa ttcccgcgtg cgcggggtgg gagaactggt cgcggtggag
     1801 caggtgaaag gcggcgtgca ggccaccatt cgcgtcaccg tggaaatcga aggcagtgac
     1861 aaaccggcct gcgtcgtgga tacgatctcc cgctactatc ccaagtgaat cacagaaccc
     1921 gaccattgtt tcaacggccg accgtatttt ccggcgcaaa gaattccatt caaggggaaa
     1981 gcacatgaaa gaagccgtca tcgtctccac cgcccgtacc gcgctgggca aatcgttccg
     2041 tggcgcatt
//
LOCUS       DASUEG010000984         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1759408, whole genome shotgun sequence.
ACCESSION   DASUEG010000984 DASUEG010000000
VERSION     DASUEG010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759408"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /locus_tag="VFX11_18450"
     CDS             complement(<1..2555)
                     /locus_tag="VFX11_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4940661.1"
                     /translation="MTARQQIFRVRRNYNQWVANQTLEDYALRFTAKRARRWSLARVA
                     NTALGAISFLALEAIGGAVTLSYGFDNAVVAIMVVGLIIFLTGMPIAFYAAKYGIDID
                     LLTRGAGFGYIGSTITSLIYASFTFIFFALEAAIMAMALEILLGIPQTIGYLLSSLLV
                     IPLVTHGITFISRFQLWSQPLWMALQIVPFVYIALHDIGSVQDWTQYEGLHGSGAGGL
                     NLLLFGAASSVIFSLIAQIGEQVDFLRFLPAIAEEKNGQQGKKLRWWLAMLAAGPGWI
                     IVGTLKILAGSFLAVLALKHGVLADDAADPTRMYSIAFGYITSSPDFALALAGIFVIL
                     SQLKINVTNSYAGSIAWSNFFSRLTHSHPGRVVWLVFNVAIALLLMELGIYRALESIL
                     GVYAIVAVAWVGSLVADLVINKPLGLSPPQIEFKRAHLYDINPVGFGSMILASLLGIS
                     AHLGSFGEVPQALSPYVALVSAFVLAPLIAWWTKGRYYIARMPETIFCGSEAITCCIC
                     EHAFEPEDITTCPAYGGHICSLCCSLDARCQDLCKPDARLSRQIISLLRGVLPDRITD
                     ALNSRLGHFLGLLLLIGGCIGLLLTLVYYQIPVTDADTANLIGTVLWKEFFILLIVAG
                     VVAWLFILAHESRVVAQEESQRQTRLLEEEIIAHERTDLALQKAKEVAEAANHAKSRY
                     LTGISHELRTPLNAVLGYAQLLEQDDEIPHSRREAISTIRRSSEHLADLIEGLLDISK
                     IEAGRLDLHRDQVRIRPLLDQIVQMFRLQAEAKGILFEYRNEGFLPEYVATDEKRLRQ
                     ILINLLSNAIKFTEWGGVSFSVRYRSQVAEFTIRDSGVGIHKEDIERIFRPFER"
     gene            2824..>3211
                     /locus_tag="VFX11_18455"
     CDS             2824..>3211
                     /locus_tag="VFX11_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX4940662.1"
                     /translation="MLTKFPGSNKTHSSLRRRILQGMLALPAAMLLSHAALAAPSTAE
                     VNTTGLAVTDSTVKVGILHSVTGTMAISETGSVQAEKLAIDQINAMGGVLGRRIKVIK
                     EDGASDWPTFAEKAKKLLINDRCAAVF"
BASE COUNT          717 a          970 c          942 g          582 t
ORIGIN      
        1 cgttcgaagg gacggaagat gcgttcgata tcctccttgt gaatgccaac gccgctgtca
       61 cggatggtga actcggctac ctggctgcga taacgcacgc tgaacgacac gccaccccat
      121 tcggtgaact tgatggcgtt cgacagcagg ttgatcagta tctggcgcag acgtttttca
      181 tcggtggcca cgtattccgg cagaaagcct tcattgcggt attcgaacag gatgcccttg
      241 gcttccgcct gcagccggaa catttgcacg atctgatcca gcagcggacg aatgcgcacc
      301 tgatcgcggt gcagatcgag ccggcccgct tctatcttgg aaatatcgag caaaccttca
      361 atcagatccg ccagatgttc actgctgcgg cggatggtgg aaatggcctc gcggcgggaa
      421 tggggaattt cgtcgtcctg ttccagcaat tgcgcgtaac cgagcaccgc attgagcggt
      481 gtgcgcaatt catggctgat gccagtgagg taacggcttt tggcatggtt cgctgcttct
      541 gccacctcct tggctttctg caacgccaga tcggtacgtt cgtgggcgat gatttcctct
      601 tccagcagac gcgtttgccg ttgcgattcc tcctgcgcca ccacgcgact ttcgtgcgcc
      661 agaataaaca gccaggccac cacgccggcc acgatcagca gaatgaaaaa ttccttccac
      721 aacacggtgc cgatcaggtt ggccgtgtct gcatcggtga cgggaatctg gtaatacacc
      781 agggtgagca gcaaaccgat gcagccgccg atcagcagca gcagaccgag aaaatggccg
      841 agcctggaat tgagggcgtc ggtgatgcgg tccggcagca caccgcgcag caggctgatg
      901 atctggcgcg acagacgcgc atccggtttg cacaggtcct ggcagcgggc gtccagcgaa
      961 cagcacagcg agcagatgtg accgccgtag gccgggcagg tggtaatgtc ttccggttcg
     1021 aaggcgtgtt cgcagatgca gcaagtaatg gcttcactgc cgcagaagat cgtttcgggc
     1081 atgcgggcga tgtaataacg gcccttggtc caccaggcta tcaggggggc cagcacgaat
     1141 gcagacacca gtgccacata aggcgacagc gcctgcggca cttcaccgaa cgagcccagg
     1201 tgcgcgctga tgcccagcag cgatgccagg atcatggaac cgaagcctac cgggttgatg
     1261 tcatatagat gcgcgcgctt gaattcgatc tgcggcggac tcaaccccag cggcttgttg
     1321 atcaccagat ccgccaccag cgatcccacc caggccaccg ccacaatcgc gtacacgccg
     1381 agaatacttt ccagcgcgcg gtagatgccc agctccatca gcagcagcgc gatcgccacg
     1441 ttgaacacca gccacaccac ccggccggga tgactgtgcg tcagccgcga gaaaaaattc
     1501 gaccaggcga tggagccggc gtaggaattg gtgacgttga ttttcagctg cgacaggatg
     1561 acgaaaatac cggccagcgc cagcgcaaaa tccggcgacg aggtgatgta gccaaacgcg
     1621 atactgtaca tgcgggtggg atcggcggca tcgtctgcca gcacgccgtg tttcagcgcc
     1681 agcaccgcca gaaaggagcc cgccaggatt ttcagcgtgc cgacgatgat ccagcccggc
     1741 cccgccgcca gcatcgccag ccaccagcgc agttttttgc cttgctgtcc atttttttcc
     1801 tcggcaatcg ccggcagaaa gcgcaggaaa tccacctgct ccccaatctg cgcgatcagg
     1861 gagaaaatca ccgatgacgc cgcaccgaac agcaggagat tcagcccgcc tgcgccgctg
     1921 ccgtgcaggc cttcatattg ggtccagtcc tgaaccgagc cgatgtcatg cagcgcgatg
     1981 tacacaaacg gcacgatctg cagcgccatc cacaacggct gcgaccacaa ctggaaccgg
     2041 ctgataaagg taatcccgtg cgtcaccagt ggaatcacca gcagcgaact gagcaggtag
     2101 ccaatggttt gggggatacc aagcaggatt tccagcgcca tggccatgat ggctgcttcc
     2161 agcgcgaaga aaataaacgt aaacgaggca tagatcagcg acgtgatggt ggagccaata
     2221 tagccaaagc ctgcaccacg agtgagcaaa tctatatcga tgccatattt tgcagcataa
     2281 aaggcgatgg gcatgccggt gaggaagatg atcaggccca ccaccatgat ggcaaccacc
     2341 gcattgtcga agccgtagct gagggtgacc gcgccgccga tggcttccag tgccagaaaa
     2401 gaaatcgccc ccagcgcggt gttcgccacc cgcgccagcg accagcgccg cgcccgcttg
     2461 gcggtaaaac gcagggcgta atcctccagg gtctggttgg ccacccactg gttgtagttg
     2521 cggcggacgc gaaaaatctg ctggcgagcg gtcatggtcg ggttcgggtc tacttcagct
     2581 gggcttccgg attggcctga ctggaacaat gacgcaagtt gcataccagc caaagccggt
     2641 taatgaaaag gccggccacc tgcgcaaaat accccgtcca ccctgccagc cctatgcgtc
     2701 atttgacgta gttcgctgca atgccctggg gcatttcccg aattggcgcg cgcagtcagg
     2761 caatcaatac tcggtcccga cacacggtgt cccattccga tcgattgcct gagaggttgt
     2821 accatgctga caaaattccc tggctccaac aaaacccact cgtcgctgcg gcgacggata
     2881 ctgcagggca tgctggcctt gcctgccgcc atgctgctca gccacgcggc attggctgcg
     2941 ccgtccactg ccgaggtgaa caccaccggt ctggcggtga ccgacagcac cgtgaaagtg
     3001 ggcattctgc attccgtcac cggcaccatg gcgatcagcg aaaccggatc ggtgcaggcg
     3061 gaaaagctcg ccatcgacca gatcaacgcc atgggagggg tgctgggccg caggatcaag
     3121 gtgatcaagg aggacggcgc ctcggattgg ccgaccttcg ccgaaaaggc gaagaagctc
     3181 ctgatcaacg atcgctgcgc cgccgtgttc g
//
LOCUS       DASUEG010000985         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1008566, whole genome shotgun sequence.
ACCESSION   DASUEG010000985 DASUEG010000000
VERSION     DASUEG010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008566"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFX11_18460"
     CDS             <1..478
                     /locus_tag="VFX11_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX4940663.1"
                     /translation="GKMTDYALVLMHRLAKSPSLQLRMEELAVETRLGLPTVRKLMRQ
                     LVNAGLVRSERGAKGGYQLARFPELITIAHIVAAVEGPLAITECCDDDGGCELSGGCD
                     VESQMPTLNELITLMLGSISLTDMGRLCGNVQGNLGVLLERILERQQFNTDSAMPA"
     gene            611..1384
                     /locus_tag="VFX11_18465"
     CDS             611..1384
                     /locus_tag="VFX11_18465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4940664.1"
                     /translation="MSTLPRMFTRKLAVPVALAAALSGCAADGSFDTSAAMNVGAGVL
                     QAATLSEESVKQTASLAAQQMDSENKVPPASNAYSKRLAKITQGMTNIDGLDLNFKVY
                     MSEEINAFAMADGTVRVYSGLLDAMPDDQALAVIGHEVGHVKLKHSYQQMQQKLLTDS
                     AFAAAISVGGTVGTLTQSQLGQLGYAAVNAQFSQKDELEADQYAVATLRKLGKDPYAM
                     KRAIETLQAKYGSGGGFLSSHPSNDKRVEKIQAAIDGKK"
     gene            1500..2723
                     /locus_tag="VFX11_18470"
     CDS             1500..2723
                     /locus_tag="VFX11_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX4940665.1"
                     /translation="MESAYATVSRIANETYGHSRRIMPEQPWWENLESSFHKFPDDFD
                     LDLPTQLLVDGTVVVDLAVRTAVAGVVSTLALPLSANPFRLKQDMEDSALYREIASSH
                     DASRFFRFPNVKVDMQERKPGLIRFNPPDGQCRVLTFKSPFQPVNPRLHDRYLKNERN
                     RHAIAQYWTHNCGPRPTICVVHGFMADPYWVNSLFLGLPWFYRLGYDILLYTLPFHGA
                     RQGPFSPFSGHGYFANGISHIAETVAHSIYDFRIFLNYLERIGVPRFGVTGISLGGYT
                     SAVAAAVDERLHFAIPNVPVVSLLDLILQWFPAGNLIKAAMRLSGVSIKEARHISAVH
                     CPLTYPVKLPKERLMIIGGAGDRFAPPKHARLLWDHWNRPDIHWFPGNHIIHLDQGRY
                     LKDVAAFLQECGFAE"
     gene            complement(2786..2917)
                     /locus_tag="VFX11_18475"
     CDS             complement(2786..2917)
                     /locus_tag="VFX11_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940666.1"
                     /translation="MYVDEAVIAGMLALISSLAVGGYVLYFMLHDMKMKTKGKMKAH"
BASE COUNT          625 a          958 c          910 g          626 t
ORIGIN      
        1 cggcaaaatg acagattacg cattggtgct catgcaccgg ctggccaagt cgccgtccct
       61 gcagttgcgt atggaggaac tggcggtgga aacccggctg ggtctgccga cggtgcgcaa
      121 gctgatgcgg cagctggtga atgcgggact ggtgcgctcc gagcgcggcg ccaagggcgg
      181 ctaccagctg gcccgttttc cggaactgat caccatcgcc catattgtcg cggcggtgga
      241 agggcccctg gccatcaccg aatgctgtga cgatgacggc ggctgcgagc tgtccggcgg
      301 ttgcgacgtg gaatcgcaga tgcccactct caacgaactc atcaccctga tgctggggtc
      361 catctccctc accgatatgg ggcgcctctg tggcaatgtg cagggcaacc tgggcgtgct
      421 gctggaacgc attctggaac ggcagcaatt caacaccgac agtgccatgc cggcctgacc
      481 ccgtctggcc gcaattccgg tattttcacc ggttcgcctg ccggccccga tctgccataa
      541 tcccgcttcc ttatttcccc tgattcgaat cgccagaatc gaaacctacc tgataaagag
      601 gatgctcagg atgtcgacct tgcccaggat gttcacccgc aaattggccg ttcccgttgc
      661 cctggccgcc gcgctttccg gctgcgccgc cgacggttcc tttgatacca gcgctgccat
      721 gaatgtgggc gccggcgtgc tgcaggccgc cacccttagc gaagagagcg tcaagcagac
      781 cgcctctctc gccgcccagc agatggatag cgagaacaag gtacctccgg ccagcaatgc
      841 ctacagcaag cggctggcga agatcaccca gggcatgacc aacattgacg gcctcgatct
      901 gaacttcaag gtgtacatgt cggaggaaat caacgccttc gccatggcgg acggcacagt
      961 gcgggtctat tcgggcctgc tggatgccat gccggacgat caggcgctgg cggtcattgg
     1021 ccacgaagtg ggccacgtca aactcaagca cagctaccag cagatgcagc agaagctgct
     1081 gacggattcc gcctttgctg ccgccatttc cgtgggcggc accgtgggta cgctgaccca
     1141 gtcgcaactg ggccagctgg gttatgcagc ggtcaatgca cagttctcgc aaaaggatga
     1201 actggaggcc gaccagtacg ctgtagccac cctgcgcaaa ctgggcaagg acccctacgc
     1261 catgaagcgc gccatcgaaa ccctgcaggc caaatacggt tccggcggtg gtttcctcag
     1321 ttcccacccg tccaacgaca agcgggtcga gaaaatccag gctgccatcg acggcaaaaa
     1381 gtaagcggca aacagcagta cagatgctgc gctgaatgca caaggccgcg acccaccgtc
     1441 gcggccttgt cgttttggct atactgaaat gcataagcca ttgaaacggc gaggtgctta
     1501 tggaatccgc ctacgcgacc gtgtcccgca ttgccaacga gacctacggc cactcccgcc
     1561 gcatcatgcc ggagcagccc tggtgggaaa atctcgaaag cagtttccac aaattccccg
     1621 acgattttga tctggacctg ccgacgcaac tgctggtgga tggcaccgtg gtagtggatc
     1681 tggcggtgcg cacggcggtg gcgggtgtgg tgtccacgtt ggcgctgccg ctgtcggcca
     1741 atccgttccg gctgaagcag gacatggaag acagcgcgct gtaccgggaa atcgccagca
     1801 gccatgacgc cagccgcttc ttccgctttc ccaacgtgaa agtggacatg caggagcgca
     1861 agcccggcct gatccgtttc aatccgccgg acggccagtg ccgcgttctc accttcaaaa
     1921 gcccgttcca gccggtgaat ccccgtctgc acgaccgcta tttgaagaac gaacgcaacc
     1981 gtcacgccat cgcccagtac tggacccaca actgcggccc gcgccccacc atctgcgtgg
     2041 tgcacggctt catggccgac ccgtactggg tgaacagcct gttcctggga ttgccctggt
     2101 tttaccggct gggttacgac attctgctgt acacactgcc attccatggc gcgcgtcagg
     2161 ggccgttctc gcccttcagc ggtcacggct atttcgccaa tggcatttcc catattgcgg
     2221 aaacggtggc gcattccatt tacgatttcc gcattttcct gaattacctg gagcggatcg
     2281 gcgtgccgcg tttcggcgtc accggcatca gcctcggcgg ttacaccagc gcggtggcgg
     2341 cagcggtgga cgagcgtctg cattttgcca ttcccaacgt gccggtggta agcctgctcg
     2401 acctgatcct ccagtggttt cccgccggca atctgatcaa ggcggccatg cggttgagcg
     2461 gtgtgtcgat caaggaagcg cgccacatct cggccgtgca ttgcccgctg acgtacccgg
     2521 tgaagttgcc gaaagagcgg ctgatgatca tcggcggcgc cggtgaccgc ttcgcgccgc
     2581 ccaagcatgc gcgtctgttg tgggatcact ggaaccggcc ggacatccac tggtttccgg
     2641 gcaatcacat cattcacctg gaccaggggc gatatctgaa agacgtggcg gcgtttctgc
     2701 aggaatgcgg ttttgccgag taaaaaagga agcgggggtg tcgactgggt cggcaccccc
     2761 gctggagaag gtcagtaagc ctggttcagt gggctttcat cttgcccttg gttttcatct
     2821 tcatgtcatg gagcatgaag tacaacacat agccgccgac agccagtgaa ctgataaggg
     2881 ccagcattcc ggcgataact gcttcatcaa cgtacataaa ttccctctct gtatcttatt
     2941 agtgattgtt tttgtcgata ctgctgaccg ggatcaatgc ccggtattcc agaatgtagt
     3001 ccagcggctg gacgaaagca cccccatgta actatacagt ctgaatacct ctttccttat
     3061 acccaaaatc tccaccggtg acgcgggggc ggcgggctag actgaagtct ggagccaat
//
LOCUS       DASUEG010000986         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1560827, whole genome shotgun sequence.
ACCESSION   DASUEG010000986 DASUEG010000000
VERSION     DASUEG010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560827"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2339
                     /locus_tag="VFX11_18480"
     CDS             <1..>2339
                     /locus_tag="VFX11_18480"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4940667.1"
                     /translation="HLTAVVAGLHNVGAAIQWKNLFIDGKHTRVDLPTYPFQKQSYWV
                     EEIRIGNYSKQSGMSFARMLYSTDWKETALADEANVNYDDVVVIGNKPAWLAALQAQH
                     ANVLVLDANSTAETIKSAVAPLQEKAKAAGKVLPVLLSAPVMPTDSNALVDTVNAAIK
                     PVLATARALVAGSDSTNPVRLWCVSENAYSFGKEGGTDNINLAAYPQLGFAKGMALEA
                     GELWGGILDVSGSADEQAAAITAELNGQTPEDAVKYVGAVRSVLRLQSDRPAQTSLSN
                     LNDKATYLVTGGLGGLGLYVAEALALSGAKHLVLMSRRGDLASLQGERREQIEKLQQK
                     GVDIRVANVDVADRNAVAGLIEELAHGEFPLKGIVHGAGVNDVVLAHEMQPEQWLNVT
                     QSKVQGGWNLHQASLNVELDFFVVFSSIASVWGSGGMAHYAAANHFLDGLVDYRLAQG
                     LPAAAFNWGPWGGAGMAVGEAAEEAERRGLRPFDPQSGIELLSKSWKGGKAHQVMVDV
                     TWDRFREILEMRRPHPLFGALGRSLSTASGVTGEKSAFFKSLYPLEPTERATQIVGYL
                     QGLLGRVVGKAEGETVDPEMPLMDLGIDSIMALEIKKQLEADTGQAMRATLIFDYPTI
                     NKIAEYFSVALYGEEEGAAAVSGGVFHGEPIAIVGIGCKMPMAPNGPGDFWKLLSNGE
                     CGINDAPSQRWDLDRYLDKDENAPGKTYTLSAGLVDDIESFDGKMFGIAPRELESMEP
                     QQRLVLEHAWTALENAGYSPAAMNGTRTGVFVGVGANEY"
BASE COUNT          483 a          654 c          764 g          438 t
ORIGIN      
        1 atcacctgac cgctgtggtg gccggcctgc ataacgtagg tgctgcgatc cagtggaaaa
       61 acctgttcat cgatggcaag catacccgcg tcgatctgcc cacctatccg ttccagaagc
      121 agagctactg ggtggaagaa atccgcatcg gcaattacag caagcagtcg ggcatgtcgt
      181 tcgcccgcat gctgtacagc acggactgga aagaaaccgc gctggccgac gaagccaacg
      241 ttaactatga cgatgttgtc gtaatcggca acaaacccgc ctggctggcc gcgctgcagg
      301 cgcagcatgc caatgtgctg gtgctggacg ccaacagcac ggcagaaacc atcaagagcg
      361 ccgtggcgcc gctgcaggaa aaagccaagg ctgctggcaa agtgctgccg gtgctgctga
      421 gtgcgccggt gatgccaacc gacagcaacg cgctggtcga taccgtcaac gccgccatca
      481 aaccggtgct ggcgaccgct cgcgcgttgg ttgcaggcag cgattccacc aacccggtgc
      541 gcctgtggtg cgtgagtgag aacgcctatt cgttcggaaa agagggggga actgacaaca
      601 tcaacctggc cgcgtatccg caactgggct ttgccaaagg catggcgctg gaagccggtg
      661 aactctgggg cggcattctg gatgtgagcg gcagtgccga cgaacaggcc gctgcgatca
      721 ccgccgagct gaatggccag acgcccgaag acgcagtgaa gtacgtaggc gccgtgcgtt
      781 ccgtgctgcg tctgcaatcg gatcgtcctg cgcagaccag cctgtccaat ctgaacgaca
      841 aggccaccta tctggtaacc ggcggcctcg gcggtctggg cctgtatgtc gcggaagcac
      901 tggcgctgtc cggtgcgaaa catctggtgc tgatgagccg tcgtggcgat ctggcatcgc
      961 tgcagggtga gcgtcgcgag cagatcgaaa aactgcaaca gaaaggcgtg gatatccgcg
     1021 tcgccaatgt cgatgtggca gaccgcaatg cggtggccgg cctgatcgag gaactggcgc
     1081 acggcgaatt cccgctgaaa ggtatcgtgc acggcgccgg tgtgaacgat gtggtgctgg
     1141 cccatgaaat gcagccggag cagtggctga atgtaaccca gtcgaaagtg cagggtggct
     1201 ggaacctgca tcaggcatcg ctgaacgtgg agctggattt cttcgtggtg ttctcgtcca
     1261 tcgcctctgt gtggggttcc ggtggcatgg cgcattacgc agcggcgaac catttcctcg
     1321 acggtctggt ggattaccgt ttggcgcaag gcctgccggc tgctgcgttc aactggggtc
     1381 cctggggtgg cgcgggcatg gcagtgggcg aagcggcgga agaagccgag cgtcgcggcc
     1441 tgcgcccgtt cgatccacag tccggtattg aattgctgag caaatcctgg aaaggcggaa
     1501 aagcgcatca ggtgatggtg gatgtgacct gggaccgttt ccgcgaaatt ctggaaatgc
     1561 gtcgtccgca tccgctgttt ggtgcgctgg gccgttcact gtcgactgcg tctggcgtca
     1621 ccggcgaaaa atccgcgttc ttcaagtccc tgtatccgct ggagccgacc gagcgtgcca
     1681 cgcaaatcgt tggctatctg caaggtctgt tgggtcgggt ggtaggcaag gccgaaggcg
     1741 aaaccgtcga tccggaaatg ccgctgatgg atctgggtat cgattccatc atggcgttgg
     1801 aaatcaagaa gcagctggaa gccgacaccg gccaggccat gcgcgcgacg ctgattttcg
     1861 attatccgac cattaacaag atcgccgagt acttcagcgt tgcgctgtat ggcgaggaag
     1921 aaggtgcggc tgccgttagc ggcggcgtct tccacggcga gccgattgca attgtcggta
     1981 tcggctgcaa gatgccgatg gcacccaacg gcccgggcga tttctggaaa ttgctgagca
     2041 atggcgagtg cggcatcaat gacgcaccga gccagcgctg ggatctggac cgttacctgg
     2101 acaaagatga aaacgcgccg ggcaaaacct acacgctgtc tgccggtctg gtggatgaca
     2161 ttgaatcctt cgacggcaag atgttcggca ttgcgccgcg cgaactggaa agcatggaac
     2221 cgcagcaacg tctggtgctg gaacacgcct ggaccgcgct ggaaaacgcc ggctattccc
     2281 cggctgccat gaacggtacc cgcaccggtg tgttcgtcgg cgttggcgcc aacgaatac
//
LOCUS       DASUEG010000987         4923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_381490, whole genome shotgun sequence.
ACCESSION   DASUEG010000987 DASUEG010000000
VERSION     DASUEG010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381490"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFX11_18485"
     CDS             <1..195
                     /locus_tag="VFX11_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HEX4940668.1"
                     /translation="IADAQVVQIEYPVLQYPVKVSSINLDKTPLLQGTLLGIKGQYLM
                     FDTGVINIRKYSGYEVEWSA"
     gene            337..1881
                     /locus_tag="VFX11_18490"
     CDS             337..1881
                     /locus_tag="VFX11_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4940669.1"
                     /translation="MRFLLDLPLKFKFWLVNLVSFAGMLLLVLAAIVDYRQGLLEQAQ
                     QQARPLLALQAQLMQAQPERTPIHLPSGFIIADDDVRWLGDAEISGRDLQQRVANLPL
                     RGGFQFDDRSGSGTLGRWWADLFGGDALRYVALQSTSGGQALGMIVDVPSIMALSWQK
                     FFYYGGVVFLMMLLVLAASQALIQFVSKPVAALRDAMRQVHEQGDLSVRAVCESSDEV
                     GQMTLAFNNMVKDVSRIVTEIRAAAFTMDSMSEHLARESSSNVQSVEVQQAETEQLAA
                     AITEMASTSQDVQRNANENNVRSMESVKVARSGNQQVDFVVTAITRLATDIRDGAAVV
                     HKLADETSAIGTALDVIKSIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAQR
                     VQDSTDQIKTMLDRLQQSSMQAVNVMTERSSDAGRCVEQAQQAGAVIHEIAGNAQSIN
                     DSNTQIAVSTTEQSSTVESVNQNVIKLRDEMEAVFTSVKRNADAARTLAELSTRMMRS
                     IEHLKL"
     gene            complement(1882..2325)
                     /locus_tag="VFX11_18495"
     CDS             complement(1882..2325)
                     /locus_tag="VFX11_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX4940670.1"
                     /translation="MAYLSLFLTAFAAATVLPLSSEALLLALLQQGFSPALLWLVATT
                     GNTLGSVVNWGMGRFLLHFLTARWQQMRWVPIKPAQLETAQRLFQRYGVWSLLFAWLP
                     VVGDALTLAAGMLRVNFPVFVTLTAIGKGARYAVLLWLAEKAFAG"
     gene            complement(2362..4497)
                     /gene="pta"
                     /locus_tag="VFX11_18500"
     CDS             complement(2362..4497)
                     /gene="pta"
                     /locus_tag="VFX11_18500"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX4940671.1"
                     /translation="MKTLLLAPTESGVGLTTICLGLLHALDQKGLRVAFCKPFGHLKE
                     DGGDRSVQLIQHYTGLNPPAPLPCTRAAQLLAANQADLLMEEVISLRQHTLASLTYKP
                     DVLVLEGVNTSADDPVLAQLNALMARSLDAQVILVTTPHAQSITELNQRLEASATHFG
                     GIGDRRVVGCILNMINAPLDRHGHIRPDIDNPSFNTALSWSQIQTQLPILQRQDFHLA
                     GGIPWDADLTAPRTWDVAQYLGATILAAGEIKLRRVLHVTIATQRVGNLLQELQPGTL
                     VITTGDREDVVVAAALAQLNGVSLAGVLFTQPADLRQDALAFCRHAIAKGLPLLQVPQ
                     DSYQMINLLPGFDNKVPVDDDIRIRHVMETVAQNLDKIWLAKLLSSKRELRLSPAAFR
                     YSLVQRARASHKTIVLPEGDEPRTIKAAVTCFHRGIARCQLLGNPDTIRQVAAANAIA
                     LPPELEIIDPVPLRETYLGLLLSLRKHKGLTEERAQQELEDNVVLGTLMLHQAKVDGL
                     VSGAVHTTANTIRPALQLIRTKPDIKIVSSIFFMCLPEQVVIFGDCAINPDPNAEELA
                     DIAIASADSARAFGVTPRVAMISYSTMESGTGSDVDKVREATRIAQSKRPDLLIDGPL
                     QYDAAMIPNVGASKAPGSKVAGHATVLIFPDLNTGNTTYKAVQRSAQVVSIGPMLQGL
                     KKPVNDLSRGATVDDIIYTIALTAIQAQE"
     gene            complement(4541..>4923)
                     /locus_tag="VFX11_18505"
     CDS             complement(4541..>4923)
                     /locus_tag="VFX11_18505"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="AckA utilizes acetate and can acetylate CheY which
                     increases signal strength during flagellar rotation;
                     utilizes magnesium and ATP; also involved in conversion of
                     acetate to aceyl-CoA; also known to act on propionate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX4940672.1"
                     /translation="AGLKGVSGIGNDMRELLQAASQGHAGAQLAVNLFCYRLAKTIAS
                     YLVPLGRIDAIAFTGGIGENAAPVRAQVIELLQGLGFRIDPEKNNARSQEPRNIAPAG
                     APAVLVIPTREEWMIARQTAQLLA"
BASE COUNT          915 a         1375 c         1614 g         1019 t
ORIGIN      
        1 attgccgatg cgcaggtggt gcagatcgag tatccggttc tgcagtatcc ggtgaaagtg
       61 agcagcatca atctggacaa gacgccgctg ttgcagggca cgttgctggg gatcaagggc
      121 cagtacctga tgttcgacac cggcgtgatc aatatccgca agtattccgg ctacgaagtg
      181 gagtggtctg cctgatcgtt gatcgggttc tgtggacaat ccgttttata tccagctgct
      241 atagttattc cgggtgttgt cgcgcggcat ttcggtgctt ttttagcgcg tccggttata
      301 aatggcacga atgatattct gcacggtggt gttcccttgc gctttctgct ggatcttccc
      361 ctcaaattca aattctggct ggtaaatctg gtcagcttcg ccggcatgct gctgctggtg
      421 ctggcggcca ttgtcgatta tcggcagggc ttgctggagc aggcgcagca gcaggccagg
      481 ccactgctgg cgctgcaggc gcaactgatg caggctcagc cggagcgtac gccgatccat
      541 ttgccctccg gtttcatcat tgcggacgat gatgtgcgct ggctgggtga tgccgagatt
      601 tccggccgtg acttgcagca gcgggtggcg aatttgccgt tgcgcggtgg cttccagttt
      661 gacgacagat ccggctcggg cacgctgggt cgctggtggg cggatctgtt tggcggtgat
      721 gcgctgcgct atgtcgcact gcaatccacc agcggcggtc aggcgctggg gatgattgtc
      781 gacgtgccgt cgataatggc gttgagctgg cagaaatttt tctattacgg tggcgtggtg
      841 ttcctgatga tgttgctggt attggcggcg tcgcaggcgt tgatccagtt tgtatcgaaa
      901 ccggtggcgg cgttgcgtga tgccatgcgc caggtacacg agcagggtga tctgtcggtg
      961 cgcgcggtgt gcgaatcgtc cgatgaagtg gggcagatga cgctggcatt caacaacatg
     1021 gtgaaggatg tgtcgcgcat tgtcaccgag atccgcgcgg ccgcgttcac catggattcg
     1081 atgtcggagc acctggcgcg ggaatccagc agcaatgtgc agagtgttga agtgcaacag
     1141 gctgaaaccg aacagctggc ggcagccatt actgaaatgg ccagcaccag tcaggatgtg
     1201 cagcgtaacg ccaacgagaa caatgtgcgt tcgatggaat cggtgaaagt agccaggagc
     1261 ggtaaccagc aggtggattt tgtggtgaca gccattacgc gtctggccac cgatattcgc
     1321 gatggcgcag cggtggtaca caagctggca gatgaaacca gtgccatcgg caccgcgctg
     1381 gatgtgatca agtcgattgc tgaacagacc aatctgctgg cgctcaacgc tgcaattgaa
     1441 gctgcccgcg ccggcgagca gggccgtggt tttgccgtgg tggcggatga agtgcgcagt
     1501 ctggcgcagc gggtgcagga ttccactgac cagatcaaga ccatgctgga tcggttgcaa
     1561 cagagttcga tgcaggcggt aaacgtgatg acggagcgtt ccagcgatgc cggtcgctgc
     1621 gtggagcagg cgcagcaggc gggtgcggtg attcacgaaa ttgccggcaa tgcccagagc
     1681 atcaacgatt ccaacaccca gatcgcggtg agcaccacgg aacaaagcag cacggtggaa
     1741 agcgtaaacc agaacgtgat caagttgcgc gatgaaatgg aagcggtgtt caccagtgtg
     1801 aagcgcaacg cggatgccgc acgtacgctg gcggagcttt ccacccggat gatgcgttcg
     1861 atcgagcacc tgaagctgta atcaacccgc gaaggctttt tccgccagcc acagcaatac
     1921 tgcgtagcga gcgcccttgc cgatcgcggt gagagtcaca aacaccggga aattcacccg
     1981 caacattcca gctgccagtg tcagtgcatc gcccaccacc ggcagccatg cgaacagcag
     2041 gctccacacg ccataacgtt gaaacaggcg ttgcgcggtt tccaattgcg ccggcttgat
     2101 tggcacccag cgcatttgct gccagcgcgc tgtcagaaaa tgcagcagga agcgccccat
     2161 tccccagttc accaccgatc ccagcgtgtt gccggtggtg gccaccagcc agagcagcgc
     2221 cggtgaaaat ccctgttgca gcagcgccag cagcagtgct tccgacgaca gcggcagcac
     2281 ggtggccgct gcaaatgctg tcagaaacag tgacaggtac gccatcaacg atcaggcccg
     2341 cgcatcatgg ctgaatgctg ctcattcctg cgcctgaatg gcggtgaggg cgatggtgta
     2401 gatgatgtcg tccaccgtgg cgccccgcga cagatcgttc accggttttt tcaatccctg
     2461 cagcatggga ccgatgctga ccacctgcgc gctgcgctgc acggccttgt aggtggtgtt
     2521 gccggtattg aggtcgggga aaatcagtac ggtggcatgg cccgccacct tgctgcccgg
     2581 tgccttgctg gcgccgacgt tgggaatcat cgcggcgtca tattgcagcg gaccgtcgat
     2641 cagcaggtcg gggcgttttg actgcgcgat gcgggtagcc tcacgcacct tgtccacgtc
     2701 gctgccggtg ccggattcca tggtgctgta gctgatcatt gccacccgcg gtgtgacgcc
     2761 gaaagcacgg gcggaatcgg cgctggcaat ggcgatgtcc gccagttctt ctgcattggg
     2821 atcgggattg atggcgcagt cgccaaagat caccacctgt tccggcagac acatgaaaaa
     2881 tatcgacgac acgatcttga tgtccggttt ggtgcggatc agttgcagcg ccggacggat
     2941 ggtgttggcg gtggtgtgca cggcgccaga taccagtccg tccaccttgg cctgatgcag
     3001 catcagtgtg cccagcacca cgttgtcttc cagttcctgc tgcgcgcgtt cttccgtcag
     3061 tcccttgtgt ttgcgtagtg acagcagcag gccgagatag gtttcccgca gcggcaccgg
     3121 atcaatgatt tccagttccg gcggcagggc aatcgcgttg gcggcggcga cctggcgaat
     3181 ggtatcggga ttgcccagca gctggcagcg ggcgatgccg cgatgaaaac aggtgacggc
     3241 cgccttgatg gtgcgcggct cgtcgccctc gggcaggaca atggttttgt gcgaggcgcg
     3301 ggcgcgctgt accaggctgt aacggaacgc cgccggtgac agccgcagtt cccgcttgct
     3361 tgacagcagt ttcgccagcc agattttgtc gagattctgc gccacggttt ccattacgtg
     3421 gcggatgcgg atgtcgtcat ccaccggcac cttgttgtcg aaaccgggca gcaggttgat
     3481 catctggtag ctgtcctgcg gcacctgcaa cagcggcaat ccttttgcga tggcatggcg
     3541 gcagaacgcc agcgcatcct gccgcagatc ggccggctgg gtgaacagca cgccggccag
     3601 cgacacgcca ttcagctgcg ccagtgcggc ggccaccact acgtcttcgc ggtcgccggt
     3661 agtgatcacc agtgtgccgg gctgcaattc ctgcagcagg ttgcccacgc gctgggtggc
     3721 gatggtgaca tgcagcacgc gccgcagttt gatttcaccg gcggccagaa tggtggcacc
     3781 cagatattgc gccacatccc aggtgcgcgg cgcggtgaga tcggcgtccc acggaatgcc
     3841 gccggccaga tggaaatcct ggcgttgcag gatgggcagt tgggtctgga tctgcgacca
     3901 cgacagtgcg gtattgaacg acggattgtc gatgtccggg cggatgtggc cgtgacggtc
     3961 caggggtgcg ttgatcatgt tgaggatgca gcccaccacg cgccggtcgc caatgccgcc
     4021 gaagtgggtg gcgctggcct ccagccgctg gttcagctca gtgattgact gggcgtgtgg
     4081 agtggtcacc agaatgacct gcgcgtccag cgagcgcgcc atcagcgcgt tgagttgcgc
     4141 cagcaccgga tcatcggcgc tggtgttgac gccttccagc accagcacgt caggcttgta
     4201 ggtcagtgat gccagtgtgt gctggcgcag tgaaatgact tcttccatca gcaggtcggc
     4261 ctgattggca gccagcagct gtgcggcccg ggtgcagggc agcggtgccg gtggattcaa
     4321 cccggtgtaa tgctggatca gttgcaccga gcggtcgccg ccatcttcct tgagatggcc
     4381 gaagggtttg cagaacgcca cgcgcagacc tttctggtcc agcgcgtgca acaggcccag
     4441 gcagatggtg gtaagtccga ctccggattc ggtgggcgcc agcagcaggg ttttcatcgg
     4501 tggattcctt gtcaaacgtt caggccattt ctgtgtcagg tcaggcaagc agttgtgcgg
     4561 tctggcgggc gatcatccat tcctcgcggg tggggatcac cagcactgca ggcgcgccag
     4621 cgggtgcaat gttgcgtggt tcctgtgagc gcgcgttatt tttttccgga tcgatgcgga
     4681 agccaagccc ctgcaacagt tcgatcacct gcgcgcgcac cggtgcggcg ttttcaccga
     4741 tgccaccggt gaaggcgatg gcgtcgatac ggcccagggg taccagatag gacgcaatgg
     4801 tttttgccag ccggtagcag aacagattga cggccagttg cgcgcccgcg tggccctggc
     4861 tggcggcctg cagcaattcg cgcatgtcgt tgccgatgcc ggacacacct ttcagcccgg
     4921 ctt
//
LOCUS       DASUEG010000988         5465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2018458, whole genome shotgun sequence.
ACCESSION   DASUEG010000988 DASUEG010000000
VERSION     DASUEG010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018458"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..979)
                     /locus_tag="VFX11_18510"
     CDS             complement(377..979)
                     /locus_tag="VFX11_18510"
                     /inference="COORDINATES: protein motif:HMM:NF040319.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HEX4940673.1"
                     /translation="MSNPTEIVSRWIDAQPEGTLIRPRDLEHLVNRAAGSLGKARAVD
                     ACGVRRLRCGEGQPLRPGSAADRQDGPISREHHWARHRPSLRGSGQCPGLTKQVPVRQ
                     IYLTDGRSRTLTLGKQIIEIRHVPKWMLVLGDSLVGDVVRALEWLGPDLAEQAVGKLA
                     GRIGQEDWRVVLGIRHSLPAWMAAAIQKALPTSVPVSFSR"
     gene            complement(963..1178)
                     /locus_tag="VFX11_18515"
     CDS             complement(963..1178)
                     /locus_tag="VFX11_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX4940674.1"
                     /translation="MDVVHLNQKQLAARWCISEATMECWRSEGIGPKFLKLCGRVLYC
                     QVDIEAYEESCLQVSTTQVAVAHVQSN"
     gene            1489..1749
                     /locus_tag="VFX11_18520"
     CDS             1489..1749
                     /locus_tag="VFX11_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4940675.1"
                     /translation="MTSIRSPQQLGDALRAARKQLGLTQPQLALAAGVGVRFIVDLEA
                     GKPTLRLENVLRVIDALGGEVQLSGLPSSVTNDQREGGDHGA"
     gene            1739..3034
                     /locus_tag="VFX11_18525"
     CDS             1739..3034
                     /locus_tag="VFX11_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX4940676.1"
                     /translation="MAHELEVWLFADRVGTLSLVDGRLNFGYAPGWLSHKDAVALSAS
                     LPLQAEPFDDRKTRPFFAGLLPEGQMRRLIAQQFQVSGQNDFALLDHIGGECAGAVTF
                     LEPGQALPVPTRSDDVQWLSDDEVVAILDELPRRPMLAGKDGLRLSLAGAQDKLPVVF
                     DGARIGLPLNGTPSSHILKPAIHAVEDSVINEGFCMALAEAMQLKPAKSKVHFVLDRS
                     FLLVERYDRLIDTQGQRQRLHQEDFCQALGVVPEMKYQNEGGPDLVQCFDLVRSATRP
                     NAPQVLRLLDFVIFNALIGNHDAHAKNFSLLYSGKAPVLAPFYDTLSTAVYPTLTQKM
                     AMKIGSKYKFSEVQARHWEQFAEGVGFTKAQAKRRILELAKLLPATARKLQSDPGNSF
                     AGNAVVEQINTLIEQRCALTIRRLTDPAAENDAAVEPSA"
     gene            complement(3487..3699)
                     /locus_tag="VFX11_18530"
     CDS             complement(3487..3699)
                     /locus_tag="VFX11_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009562915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4940677.1"
                     /translation="MEVAHLNQKQLAARWSISEATLERWRSEGIGPKFLKLCGRVLYR
                     QVDIEAYEESCLATSTKTVVAQVGAG"
     gene            3915..4964
                     /locus_tag="VFX11_18535"
     CDS             3915..4964
                     /locus_tag="VFX11_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX4940678.1"
                     /translation="MLNTDTIQITPEILSLIARIDEFKGAWRALGTLAPDRLSALRRV
                     ATIESIGSSTRIEGSKLSDREVEQLLSNVEIKSFATRDEQEVAGYAELMDLVFSSWQD
                     IPITENHIKQLHQILLRYSEKDTWHRGNYKTNSNSVAAFDEAGAQIGIVFQTASPFDT
                     PRLMTELLVWVNQERETARLHPLLIIALCIVVFLEIHPFQDGNGRLSRVLTTLLLLQA
                     GYAYVPYSSLESVVEQSKEAYYLALRQTQGTIRTEAPNWQPWLMFFLRSLAEQARRLE
                     KKVEREKIVLAAMPELQLQIVEFAREHGRVTIGEAIKLTGASRNTLKQHFRALVERGT
                     LDQHGSGRGAWYGLH"
     gene            5353..>5465
                     /locus_tag="VFX11_18540"
     CDS             5353..>5465
                     /locus_tag="VFX11_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX4940679.1"
                     /translation="MPLTDTAIRNAKPGDKPVKLFDERGLYIEISPTGGKW"
BASE COUNT         1111 a         1638 c         1589 g         1127 t
ORIGIN      
        1 ggccatcgat cgcaagggtc tgcgggctct ttttgcgcct gttttgtggc tggccgtgat
       61 ggccgaatgg ctcgtctctg cgcggaatca ctcccgccat cacgccgcta tgctcaaatt
      121 gaattttgca aggtcgacta cataggtttg gcgcagggtt ccaccccacc cagaccccgc
      181 aggagttcga atctttccgc acgaacccgc aggaaccctc accagcccgc acggccagat
      241 ggtaggttgt tgattttcaa cgaaccgccc gatcggttcg cgcttgcgga ctgcggacgt
      301 tcaattcccc ggtaatctgg cttgcaagcg tcgttgcagt gccgtctcgt tgcgaaacgc
      361 tggcacttgc acacatctac cgactaaacg aaaccggtac cgatgtcggc agtgctttct
      421 ggattgctgc tgccatccac gccggcagac tgtgccggat gccgagtacc acccgccagt
      481 cctcctgccc gatgcgcccg gccagcttgc cgaccgcttg ctcggcaaga tccgggccca
      541 gccattcaag agctcttacg acatcgccga cgagactgtc acccaggacc agcatccatt
      601 tgggcacatg gcggatctcg atgatttgct tgccgagagt cagagtccgg gaccggccat
      661 cggtcaagta gatctgccga acgggaacct gtttggtcag cccagggcat tggccgctac
      721 cccgcaatga cggacgatgg cgcgcccagt gatgctcgcg agagattgga ccatcttgtc
      781 gatcggcggc ggaacctggc cgaagcggct ggccctcacc gcaacgtaga cgccgcacgc
      841 cacacgcatc aaccgcccgt gccttgccaa gcgagccagc tgcgcgattg accaggtgct
      901 ccaagtctcg aggcctgatc aaagtccctt ccggctgggc gtcaatccag cggctgacga
      961 tttcagttgg attggacatg ggcaacggcg acctgggtgg ttgatacctg caggcacgac
     1021 tcctcgtaag cctcgatgtc gacttgacaa tacaggacac gaccgcagag cttcaggaac
     1081 ttgggcccga tcccctcgct gcgccagcat tccatggtgg cctcgctgat gcaccagcgg
     1141 gcggccagtt gtttttggtt gaggtggaca acgtccatct tggtctcctt ccgaatgagt
     1201 tggcaggagt ccatgaaggc gctgttctgc tcaccagcca agcgatagtt gtgaccatct
     1261 catcccaatg aggggccgtg gcgcagttcg ggcatgtatt cggcgaactg agcgacattg
     1321 ggtgtaaatg agcgtcaatt tggaagcaag atacgcatat aattcccgat cgggaaatat
     1381 gcagctaatc cacggcctaa tcgcatgcag cattcccgat cgggaaatat cgcttgcaca
     1441 tagtccggtt gtggctgata atttcccgaa cgggaaactg agcaagccat gacatccatt
     1501 cgatcacccc aacaactcgg cgatgcactg cgtgccgctc gcaagcagct cgggctgacc
     1561 cagccccagt tggcgctggc ggcaggggtg ggtgtgcgct tcattgtcga ccttgaagca
     1621 ggcaaaccca ccttaaggct ggaaaacgta ctgcgggtca ttgatgcatt gggtggagag
     1681 gtccagttga gcgggttgcc atcatctgtg accaatgatc aacgcgaggg tggcgaccat
     1741 ggcgcatgag ctggaagtct ggctattcgc cgatcgcgtc ggcacgctgt ccctagtcga
     1801 tgggcgactg aactttgggt acgcccccgg ttggctgtca cacaaagacg ccgtagcctt
     1861 gtccgcctca ctccccctgc aagcggagcc gttcgacgat cgcaaaacgc gtcccttctt
     1921 tgcgggtctt ctacccgaag ggcaaatgcg ccgcctgatt gcgcagcagt tccaggtgtc
     1981 tggccagaac gactttgcgc tgctggacca catcggcggc gaatgcgccg gggccgtgac
     2041 ctttcttgag ccagggcagg ctttgcctgt gccgacacgc agcgatgacg ttcaatggct
     2101 gagcgacgac gaagtcgttg ccatcctgga tgaattgccg cgtcgcccca tgctggcagg
     2161 taaagacggc ttacggcttt ccttggctgg tgcccaagac aagttgccag tggttttcga
     2221 tggtgcgcgc atcgggctgc ccctcaacgg cacgcccagc tcccacatcc tgaaaccggc
     2281 catccacgcg gttgaagaca gtgtgatcaa cgaagggttt tgcatggcgc tggctgaggc
     2341 catgcagctc aagccagcga aatcgaaagt tcactttgtg ctggatcgct cgtttctgct
     2401 ggttgaacgc tacgaccgac tgattgatac ccaggggcag cggcaacggc ttcatcaaga
     2461 ggacttctgc caggcactgg gcgtggtgcc cgaaatgaag taccagaatg aaggtggtcc
     2521 ggatttggtc cagtgttttg atctggtgcg cagtgcgacg cgccccaatg caccgcaggt
     2581 tctgcgactg ctcgacttcg tgatcttcaa tgcgctgatc ggcaatcatg atgcgcacgc
     2641 caagaatttc tctctactgt actcaggcaa agcccctgtt ctggcgccgt tttacgacac
     2701 gctgtcgacg gcggtgtatc caacactgac gcagaagatg gcgatgaaaa tcggcagcaa
     2761 gtacaagttc agcgaagttc aggcacggca ctgggagcag tttgcagaag gcgtgggctt
     2821 taccaaggcg caggccaaac ggcgcattct ggaattggca aagttactgc ccgcgacagc
     2881 gcgcaagctc cagtctgacc ccggaaacag ttttgctggc aatgccgtgg ttgagcaaat
     2941 caataccttg attgaacaac gctgcgccct gacgattcgg cggctcaccg accccgccgc
     3001 cgaaaatgac gcagccgtcg aaccctcggc ctgaaccgtc atgcggacgc gggttcgatt
     3061 ccgtgttttg gaattgaaca ggcgctggaa agcagatggg tttgatgccc gtttccggtg
     3121 acaaccaagg ttggaaaacc tgtgtgcagg tctacaatcc agcaggcttt tcgatcgagt
     3181 tcgcaccggt tcgcagcagc ccgcagcgct tcgattccgt actttcaata atcgtttggt
     3241 gcggggtgct gccccaccca taccccgcag gagttcgaat ctttccgcac gaacccgcag
     3301 gaaccctcac cagcccgcac ggccagatgg taggttgttg attttcaacg aaccgcccga
     3361 tcggttcgcg cttgcggact gcggacgttc aattccctgg caatctggct tgtaaacgtg
     3421 gtcgccggta ccggctcgtt gcgaaacgtc ggtacttgca gatatttacc gacgaatcgg
     3481 aacatctcag ccagcaccga cctgagccac cacggtcttg gtcgacgttg ccaggcaaga
     3541 ctcctcgtag gcctcaatgt cgacctgccg gtagagcacc cggccgcaga gcttcaggaa
     3601 cttgggcccg atcccctcgc tgcgccagcg ttccagggtg gcctcgctga tgctccagcg
     3661 agcagccagt tgtttttgat tgaggtgagc gacttccatc ttggtctcca tccgaatgag
     3721 ttggcaggag tccatgaagg cgctgttccg cccgtgagcc aagtgatctc cttgcagaac
     3781 cctattcgcc gcattttctg cggagattag gccgattatt ccccgcaaga ccccaacctg
     3841 gaaagattgg gtgtaaatgg gtgtcttaat ggatatggct cgaaaatggg tgtaattggg
     3901 tgtagtattg gttcatgctg aacacggaca ccatccagat caccccggag atcctgagcc
     3961 tcatcgccag gatcgacgag ttcaagggcg cctggcgcgc cttgggtacg ctcgcgcccg
     4021 accgcttgtc ggccttgcgc agggtggcca ctatcgagag catcggctca tcgacccgca
     4081 tcgagggcag caagctgtct gaccgcgagg tggagcagct tctgtcgaat gtggagatca
     4141 agtccttcgc cacccgcgac gagcaggaag tggccggcta cgccgagctg atggatctgg
     4201 tgttctcgtc ctggcaagac attccgatca ccgagaacca catcaagcag ttgcaccaga
     4261 tcttgctgcg ctacagcgag aaggacacct ggcatcgcgg caactacaag accaactcga
     4321 acagcgtcgc tgccttcgat gaagccggcg cgcagatcgg catcgtgttt cagacagcgt
     4381 cgccgttcga cacgccccgc ttgatgacgg aactgttggt ctgggtgaac caggagcgtg
     4441 agacagcccg tctgcatccg ctgctgatca tcgccctgtg catcgtggtg ttccttgaga
     4501 tccacccctt ccaggacggc aacgggcggc tctctcgcgt gctgaccacg ctgctgctcc
     4561 tgcaggccgg gtacgcttat gtgccttaca gctcgctaga aagcgtagtt gagcagagca
     4621 aggaagcgta ctacctggcg ctgcgccaga cgcaaggcac gatccgcacc gaggcaccaa
     4681 actggcagcc gtggctgatg ttcttcctgc gctcgctggc cgagcaggct cggcgcctgg
     4741 agaagaaggt cgagcgcgag aagatcgtgc tggcagccat gccggagctg cagctacaga
     4801 tcgtcgagtt cgcccgcgaa cacggccgcg tcaccatcgg cgaagccatc aagctgaccg
     4861 gggccagccg caatacgctc aagcagcatt tccgggcatt ggtcgagcgc ggcacgttgg
     4921 accagcacgg cagtggccgt ggagcctggt acggcctgca ctgagcgatt gcgggacgcc
     4981 ggttctattc ctgattctgg aattgaactg gcccgaaatg cagacgaggt tgaacgtcct
     5041 ttccggtgac caatcagggc gaaaaacctg cttgatcgtc catggccgca gggcgcccca
     5101 gcaggctttt cgatcgagtt cgcaatggtt cgcagcagcc cgcagcgctt cgattccgta
     5161 ctttcaatag tcgtttggtg cggggtgctg ccccaccacc atttaaactt ccaacaacat
     5221 ccataatcat cctagagccc gcgtaattgc gggctttttc tttggttttc tatccagcaa
     5281 cggacagctt tgtccattga caaccggcaa aaatcagggg caggcgcgtt acccctacag
     5341 ggagttgccc caatgccact gaccgacacc gcgatccgca acgccaagcc cggcgacaag
     5401 cccgtcaagc tcttcgacga gcgcgggctg tatatcgaaa tttcgcccac cggcggcaaa
     5461 tggtg
//
LOCUS       DASUEG010000989         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_213954, whole genome shotgun sequence.
ACCESSION   DASUEG010000989 DASUEG010000000
VERSION     DASUEG010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213954"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFX11_18545"
     CDS             <1..259
                     /locus_tag="VFX11_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005300347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940680.1"
                     /translation="PIVRTFAPFVAGMSSMTHRRFMLLNCVGAVLWVVLFVYAGHYFG
                     NLPAVRQNFTLLMFGIILVSVLPMVIGFLQQWRARRVQPKT"
     gene            340..594
                     /locus_tag="VFX11_18550"
     CDS             340..594
                     /locus_tag="VFX11_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX4940681.1"
                     /translation="MPTYDYRCEANGKVYEVKHPMAVSVSTWGELRALADLSDDSIPD
                     SAPVAKVLTTGGVVKSSALKNAEAPPCMSGGCGGGRCQFG"
     gene            822..1301
                     /locus_tag="VFX11_18555"
     CDS             822..1301
                     /locus_tag="VFX11_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX4940682.1"
                     /translation="MTTLTAIRNVAEITVDPGTLLVAFASQEGDIVDQHFGSAQAFYL
                     YSVNADHAELVAHQSFGYEKKDGNEDKLKPKLLWLAGADVVYCGSIGGSATRQLVSLG
                     VNPLKVEGGPDVEELLAALQTQLRGTPEAWLTNILRRKQQDRSDNRFDAMDDEGWDG"
     gene            1503..1757
                     /locus_tag="VFX11_18560"
     CDS             1503..1757
                     /locus_tag="VFX11_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940683.1"
                     /translation="METFHLITPLEGNLKAALQLRPVPQLPDHWILQVDLFYDGTPAG
                     STSFRLSGYSRADAETVARTVRSNAFMMKEIDEFLWGESD"
     gene            1801..2103
                     /locus_tag="VFX11_18565"
     CDS             1801..2103
                     /locus_tag="VFX11_18565"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX4940684.1"
                     /translation="MYIAMNRFRIARGREQDFIAIWKNRDTHLQTVPGFKTFNLLQGP
                     SDDECTLFASHSTWESAAAFMDWTRSEAFRKAHANAGDSKGVYLGPPKFEGFESVL"
     gene            2234..>2773
                     /locus_tag="VFX11_18570"
     CDS             2234..>2773
                     /locus_tag="VFX11_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940685.1"
                     /translation="MRYHHLLPFLLLITAPALPAADLQTLALQPQTVPILRPFDGQVE
                     AINAATVSGQTSGRVAEILFDVGDAVPAGAKILSLVSEDQQGGLQQAQAALADARAGL
                     NVDRLELQRIEALFAKGVVSKAEQERAQGRVTSREAQVKNAEGALRRAQQQMAYTTVQ
                     APFAGVVSERHIELGEAVAP"
BASE COUNT          529 a          804 c          874 g          566 t
ORIGIN      
        1 gcccatcgtg cgcaccttcg cgccgtttgt ggcaggcatg agcagcatga cgcatcgccg
       61 tttcatgctg ctgaattgtg tcggggcggt actgtgggtg gtgctgttcg tgtatgccgg
      121 gcattacttt ggcaatttgc ccgcggtgcg ccagaatttc accctgctga tgttcggcat
      181 cattctggtg tcggtgctgc cgatggtgat cggattcctg cagcagtggc gggcgcgccg
      241 ggtgcagccg aaaacctgat ctgggtcgct gttcagggtg tcggtgcgtc ggccatactg
      301 gcaaaatagc ccgggttttt gttctaagga ggttttttca tgcccaccta cgattaccgt
      361 tgcgaagcca acggcaaagt ttatgaagtg aagcatccga tggcggtgtc ggtgtccacc
      421 tggggtgagc tgcgtgcgct ggcggatctg agtgacgatt cgattccgga cagcgcgccg
      481 gtcgccaaag tgctgaccac cggtggcgtg gtaaaaagtt ccgccctgaa aaatgccgag
      541 gcgccgccct gcatgtccgg tggctgcggt ggcggccgtt gccagttcgg ctgattgtcg
      601 ctggtatttc actggcacat tccctgctaa atcctctcct gtaagtgtgt tgcgcgcccg
      661 gcgggatgct cctgccagcg ccgcgcagca cgctggaaac ccacagattt gccgcatttt
      721 cgccactgtt tgaacggtgg cggagccggc cgcattgtgc ggtttccgac aaaacggcgg
      781 tcgccagcgc gacaagtctg accaacagcc aggagaatcc catgactacc ctgacagcca
      841 ttcgcaacgt cgccgagata acggtggacc cgggcaccct gctggtggcg tttgcgtccc
      901 aggaaggcga tatcgtcgac cagcatttcg gttcggcgca ggcgttttac ctgtacagcg
      961 tgaatgccga ccatgccgag ctggtggcgc accagtcgtt cggctatgaa aagaaggacg
     1021 gcaacgaaga caagctgaag ccgaaactgc tgtggctggc gggggcggac gtggtgtatt
     1081 gcggatcgat tggcggttcg gccacgcgac agctggtgtc gctgggcgtg aatccgctga
     1141 aagtggaagg tggcccggat gtggaggaat tgctggcggc gctgcagacg caactgcgcg
     1201 gcacgccgga agcctggctg acgaatattc tcaggcgcaa gcagcaggac aggagcgaca
     1261 accgcttcga tgccatggac gatgaaggct gggacggcta gcaggctgtt gaaaaactac
     1321 tgcgcttgca aatactgcgt taaaaatggg ctcaaaatcc tcatttacaa tctgtaaact
     1381 ccggtttttc gcccattttt gccttgtctt tgcggcgctc gtaacgtttt tcaacaacct
     1441 gctaggcccc gtgccaacca cgccagcgat ttaaccgcag ccattgaaaa agcccgccga
     1501 ccatggaaac ctttcacctg atcacgccgc tcgaaggcaa tctgaaagcg gcactgcaat
     1561 tgcgaccggt gccgcagctg ccggaccact ggattctgca ggtggacctg ttctacgacg
     1621 gcaccccggc gggcagcacc agcttccgtc tcagcggcta ttcccgcgca gacgccgaaa
     1681 cggtggcccg caccgtgcgc agcaacgcgt tcatgatgaa ggaaatcgac gaattcctct
     1741 ggggcgagag tgattgagtt tttttgaatc ttttgatgca gtaactgacg agggataacc
     1801 atgtacattg ccatgaaccg cttccgcatt gcgcggggcc gggaacagga tttcattgcc
     1861 atctggaaaa accgcgacac gcacctgcaa actgtgcccg gttttaaaac cttcaacctg
     1921 ctgcagggcc ccagcgacga cgagtgcacc ctgtttgcat cgcactccac ctgggaatcc
     1981 gctgctgcct tcatggactg gacccgatcg gaagccttcc gcaaggctca cgccaacgcc
     2041 ggcgattcca agggcgttta tctgggtccg ccgaaattcg agggttttga atcggtgctg
     2101 tgatccgcgc cgtttattga tccccgccaa gccttgtccg ttgccgggag gctatattcc
     2161 aaggtgaacc gttgttgccg gtctgtgctg cttgcgcgga tcggcggcat atcctcatca
     2221 cccgggaatt ctcatgcgct accaccacct gcttccattt ctgctgttga tcaccgcgcc
     2281 cgcgctgccg gccgccgatc tgcaaaccct ggccctgcaa ccgcaaaccg tgccgattct
     2341 gcgcccgttc gacggccagg tggaagcgat caacgccgcc acggtgtcgg gccagaccag
     2401 cggccgggtg gcggagatcc tgtttgatgt gggcgatgcc gtgccggcgg gcgccaaaat
     2461 actgagcctg gtgagcgagg accagcaggg tggcctgcag caggcccagg cggcattggc
     2521 cgatgcgcgg gccggactga atgtggaccg gctggaactg cagcgtatcg aagccctgtt
     2581 cgccaagggc gtggtgtcga aggcggaaca ggaacgggcc cagggccgcg tgaccagccg
     2641 cgaggcgcag gtgaaaaacg ccgagggtgc gctgcggcgc gcgcagcagc agatggccta
     2701 caccaccgtg caggcgccgt ttgccggtgt ggtgagtgaa cgtcacattg aactgggtga
     2761 agccgtggcg ccg
//
LOCUS       DASUEG010000990         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_787838, whole genome shotgun sequence.
ACCESSION   DASUEG010000990 DASUEG010000000
VERSION     DASUEG010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787838"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFX11_18575"
     CDS             <1..1320
                     /locus_tag="VFX11_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4940686.1"
                     /translation="WVARMIMMTMHFIKNDDGTPQVPFKTVYVHGLVRDAEGQKMSKS
                     KGNVLDPLDIIDGITLEELVQKRTSGMMQPQLAAKIEKQTRKHFPDGIASYGTDALRF
                     TFLSLASTGRDIKFDVGRLEGYRNFCNKLWNATRYVLMNCEGQDCGLDNSEIELSLAD
                     RWIISRLQKTEVQVVKALDEFRFDHASQAVYEFVWNEYCDWYLELSKPVLNDDSMSAA
                     AKRGTRRTLLRVLETILRLAHPMMPFITEEIWQQIAPKVGIKGPTIMLQSYPEANSNK
                     IDEAAETDIGWLQGVILAVRNIRGEMNIPPGKLLPVLCQKGSAADEKRVADYAGFLKS
                     LAKLEKISWLPAGAEAPMSAIQLVGDMQVLVPMAGLIDKNAEIARLQKEISKREQEIG
                     RVESKLGNESFVARAPADVVEKEKAKIAEHQAALVELKAQLEKMQQL"
     gene            1435..2022
                     /gene="rrtA"
                     /locus_tag="VFX11_18580"
     CDS             1435..2022
                     /gene="rrtA"
                     /locus_tag="VFX11_18580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEX4940687.1"
                     /translation="MALISVTNLLPQSLIEILAFQADSIRGGQVWRLLSCHFVHLGTN
                     HYLLNIVGFLILSVAFREDITPREEIISLLLSCLSVSAGLYCFNPEMAWYVGLSGALY
                     GIMAHYLIVGWSRTPFLSAIFILFLASKLLYQQFFPDSASTTGQLTGTAAFIGGPIAE
                     DSHLYGAITGFVTGFISLLLFHRHHVTRAEPDHAD"
     gene            1988..>2869
                     /locus_tag="VFX11_18585"
     CDS             1988..>2869
                     /locus_tag="VFX11_18585"
                     /inference="COORDINATES: protein motif:HMM:NF020481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEX4940688.1"
                     /translation="MSPAPNLTTRIEHFRSPAVRDLAWAILSPTLIAAPDASQHTTPA
                     WYEQAFLAIESHLHELDKDDSKLVQHLTTPATPRLGIWFERLWSYWLQHNGRYELLAA
                     NLQVKQDEKTRGEFDFIVRDSHADEIEHWELAIKFYLGIPPLHSANHWFGPNLQDRLD
                     LKFQHLVERQLPLSQSHAGQQICAERGWTVKRRRLISKGRLYLPGMETESHPPLPKCI
                     DTHALLGHWLTQQEFQRAARPFPAAAYRHLTKSEWMVSQARQSVSFSNILSQVQQSYP
                     VQIQVEGWGEQALRLFVV"
BASE COUNT          641 a          880 c          796 g          552 t
ORIGIN      
        1 tgggtggcgc gcatgatcat gatgaccatg cacttcatca agaacgacga cggcacgccg
       61 caggtgccgt tcaagacggt atacgtgcac ggcctggtac gcgacgccga aggccaaaaa
      121 atgtcgaagt cgaaaggcaa cgtgctcgac ccgctcgaca tcatcgacgg catcacactg
      181 gaagaactgg tgcagaagcg cacaagcggc atgatgcagc cgcagctggc ggcgaaaatc
      241 gaaaaacaga cccgcaagca tttccccgac ggcattgcct cctacggcac cgacgcgctg
      301 cgcttcacgt ttttgtcgct cgcctccacc ggtcgcgaca tcaagttcga cgtgggccgg
      361 ctggaaggct accgcaattt ctgcaacaag ctgtggaacg ccacccgcta cgtgctgatg
      421 aattgcgaag ggcaggattg cgggttggac aacagcgaaa tcgaactgag cctggcggat
      481 cgctggatca tttcccgcct gcagaaaacc gaagtgcagg tggtgaaagc actggatgaa
      541 ttccgtttcg atcacgcgtc ccaggccgtg tacgaattcg tctggaacga atactgcgac
      601 tggtatctgg aactgtccaa accggtgctg aacgacgata gcatgagtgc cgccgccaaa
      661 cgtggcaccc gtcgcaccct gctgcgcgtg ctggagacga ttctgcggct ggcgcaccca
      721 atgatgccgt tcatcaccga ggaaatctgg cagcagatcg cgccgaaagt tggcatcaag
      781 ggtccaacca tcatgctgca atcctatccg gaagcgaaca gcaacaagat cgacgaagcg
      841 gccgaaaccg acatcggctg gctgcagggt gtgattcttg cggtgcgcaa cattcgcggc
      901 gaaatgaata tcccgccggg caaactgctg ccggtgctgt gccagaaagg cagcgcggcc
      961 gatgaaaaac gcgtcgcgga ttatgcaggc ttcctgaaat ctctcgccaa gctggaaaaa
     1021 atttcctggc tgcccgctgg cgccgaagct cccatgtccg cgatccagct ggtaggcgac
     1081 atgcaggtat tggtgcccat ggccggcctg atcgacaaaa acgccgagat cgcccgcctg
     1141 cagaaggaaa tcagcaagcg cgaacaggaa atcggtcgcg tcgaaagcaa actgggtaac
     1201 gaaagttttg ttgcccgcgc tccggcggat gtggtggaaa aggaaaaggc caagatcgcc
     1261 gaacatcagg cggcgctggt ggaactgaaa gcgcagctgg agaaaatgca gcagctgtaa
     1321 tccatgaacg acaacggcgc gtcaccacaa tcagctgcag acattgcccg cgaggcacag
     1381 gccaccgccc gtgcccacgc gtttttccgg caatggtggt tttcgctgct cgtgatggcg
     1441 ctgatttccg taaccaacct gttgcctcaa agcctgatcg aaattctggc tttccaggct
     1501 gacagcatca ggggcggaca ggtgtggagg ctactgagct gtcattttgt gcaccttggc
     1561 accaaccatt atcttctcaa catcgtcggc ttcctgattc tgtcggtcgc attcagggag
     1621 gacatcactc cgcgcgaaga aataatcagt ctgctgctct cctgcctgag cgtcagtgcc
     1681 ggcctctatt gcttcaaccc ggagatggcg tggtatgtcg gactatccgg tgccctgtac
     1741 ggcataatgg cgcactatct gattgtgggc tggagccgaa cccccttcct gtctgccatt
     1801 tttattctgt ttctggccag caaacttctc tatcaacaat tcttccctga ctcggcttcc
     1861 acgaccgggc aactcaccgg cacggctgcc ttcattggcg gccccatcgc agaagacagc
     1921 cacctctacg gcgccatcac cggttttgtc accggattta tttcattgct gctgttccac
     1981 cgccatcatg tcacccgcgc cgaacctgac cacgcggatt gaacatttcc gctctcccgc
     2041 cgtgcgcgat ctcgcgtggg cgatcctgag ccccaccctg atcgctgcgc cggatgccag
     2101 ccagcacacc acgcccgcct ggtacgaaca ggcatttctc gccatcgaat cgcatctgca
     2161 tgaactggac aaagacgaca gcaaactggt tcaacacctc accacgcccg caacgccccg
     2221 gcttggcatc tggttcgaac ggctgtggtc gtactggctg cagcacaatg gccgctacga
     2281 attgctggca gccaatctgc aggtcaagca ggacgaaaaa acgcgggggg aattcgattt
     2341 catcgtgcgc gacagtcatg cggatgagat cgaacactgg gagctcgcca tcaagttcta
     2401 cctgggcatc ccacccctgc actctgcaaa tcactggttc ggccccaacc tgcaggaccg
     2461 gctggatctg aaattccagc atctggtaga acggcaactg cctctgtcgc aatcccatgc
     2521 cggacaacag atctgcgccg agcggggctg gactgtcaag cgccgccggc tgatcagcaa
     2581 gggccgcctg tacctgccgg gtatggaaac cgaatcccat ccgcccctgc ccaagtgcat
     2641 cgacacgcac gcactcctcg gccactggtt gacgcaacag gaattccagc gcgcagcccg
     2701 cccgtttccc gccgctgcct atcgccacct gaccaagtca gagtggatgg tgtcacaggc
     2761 gcgacaatct gtatcctttt ccaacattct gtcgcaagtg caacaatcgt atccggtgca
     2821 gatacaggtg gagggctggg gcgaacaagc actgcgcctg ttcgtcgtg
//
LOCUS       DASUEG010000991         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_655377, whole genome shotgun sequence.
ACCESSION   DASUEG010000991 DASUEG010000000
VERSION     DASUEG010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655377"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VFX11_18590"
     CDS             complement(<1..1916)
                     /locus_tag="VFX11_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4940689.1"
                     /translation="MKRIPFTADEAARANHPESSPETSPLVLSRRIFLHHGAYAAAGL
                     TLAVALPIGACSSPPPATPVTAGPVPTPPPPDLDANAFVRVTTDNRVIVTIKHLEMGQ
                     GAYNGLATLVAEEMDADWAQLEAQNAPADATRYNNLSWGPVQGTGGSSGLPNAYLQMR
                     EAGAAARYLLVSAAAEQWNVPRADLSVRQGRVLHAASNRSASFGELASLASRQQLPAS
                     VPLKDPADFVFIGRPTPRLDTVKTYGSARFTQDMYLPDMVTALVAHPPRFGATLAQVD
                     SSAALAVPGVLAVLPIPSGVAVIARSFWQAKQGRDALRLEWDESRAVFVDTAELLEQY
                     HLKTTEPGRIAAKRGQLKQGFRQSSRTLESSFEFPFLSHAAMEPMNCVVQQKPDGIEV
                     WNGCQLQTMDQQAVARVFGVQPAQVKINTLFAGGSFGRRANPYSDYVVEAAQIARAYE
                     RKVPVKLVWTREDDLRAGYFRPLYVHHLKAGLNRQGEIVAWRHRIIGQSILKGTPFEA
                     MMVKDGIDQSSVEGAANLPYTIPNFRVELTTVDPGVPVQWWRSVGSTHTAFATEHFLD
                     RIARAAGRDPVQYLLQLLKDHPRHTAVLKLAAEKSGWGKPLPKGVSLGIAVHESFNTC
                     VAQVAQVRRLNDGR"
BASE COUNT          326 a          614 c          713 g          365 t
ORIGIN      
        1 aagcggccgt cgttcagacg gcgcacctgt gccacttgcg ccacgcaggt gttgaaggat
       61 tcatgcacgg caatccccag gctgacaccc ttgggcagcg gcttgcccca gccacttttt
      121 tccgccgcca gcttcagcac ggcggtgtgg cgggggtgat ccttcagcaa ttgcaggagg
      181 tattgcaccg gatcgcgtcc ggcggcccgc gcaatccggt ccaggaaatg ctcggtggcg
      241 aaggcggtgt gggtggaccc caccgagcgc caccactgca ccggcacgcc cgggtccacc
      301 gtggtcagtt ccacgcggaa attgggaatg gtgtacggca ggttggcggc gccttccacc
      361 gaagactggt cgatgccgtc cttcaccatc atggcttcga agggggtgcc cttgagaatg
      421 gactggccga tgatgcggtg gcgccacgcc acgatctcgc cctggcggtt gaggccggct
      481 ttcaggtgat gcacatacag cggacggaaa taaccggcgc gcagatcgtc ttcccgcgtc
      541 cacaccagtt tcaccggcac cttgcgctcg taggcgcggg cgatctgggc ggcttccacc
      601 acgtagtcgg aatatgggtt ggcgcgacgg ccgaaactgc cgccagcaaa gagcgtgttg
      661 attttcacct gtgccggctg cacgccgaac acccgggcca cggcttgctg gtccatcgtc
      721 tgcagctgac agccattcca cacctcgatg ccatcgggtt tctgctgcac cacgcagttc
      781 atgggctcca tggcggcgtg ggacaaaaac ggaaattcaa agctggattc cagggtgcgg
      841 ctggactgtc tgaaaccctg tttcagctgg ccgcgtttgg cggcgatgcg accgggctcg
      901 gtggttttca ggtggtactg ttccagcagt tcggcggtat ccacgaacac ggcgcggctt
      961 tcatcccatt ccagcctgag ggcatcgcgg ccctgtttcg cctgccagaa ggagcgggcg
     1021 atcaccgcca cgccactggg aatgggcagc accgccagta caccgggcac cgccagtgcc
     1081 gccgagctgt cgacctgcgc cagggtggca ccgaagcgcg gggggtgggc caccagtgcg
     1141 gtgaccatgt ccggcaggta catgtcctgg gtgaagcggg cgctgccgta ggtcttgaca
     1201 gtatccaggc gtggcgtggg gcggccgatg aaaacgaagt cggcgggatc tttcaggggg
     1261 acgctggcgg gtagttgctg acggctggcg agggacgcca gttcaccgaa actggcgctg
     1321 cgattcgagg ccgcgtgcag cacgcggccc tggcgcacac tcagatccgc gcggggtacg
     1381 ttccactgtt ccgcggcggc ggacaccagc aggtagcgcg ctgcagcccc ggcttcccgc
     1441 atctgcagat aggcattggg caggccgctg ctgccgccgg tgccctggac cggaccccag
     1501 gacaggttgt tgtagcgggt ggcgtccgcc ggtgcgttct gcgcttccag ctgggcccag
     1561 tcggcgtcca tttcctctgc caccagggtg gccaggccgt tgtaggcgcc ctggcccatt
     1621 tccagatgtt tgatggtgac gatgacgcgg ttgtctgtgg ttacccgcac aaaggcgttg
     1681 gcgtccagat ctggcggtgg tggtgtgggg acggggcctg cggtcaccgg ggtggcgggg
     1741 ggcggcgatg agcaggcgcc aatgggcagt gccaccgcca gggtcaggcc ggcggcggca
     1801 taggcgccgt ggtgcaggaa gatccgccgg gacaatacaa gagggctggt ttcagggctg
     1861 gattccggat ggttggcacg ggctgcttca tcagcggtga acgggatgcg cttcatgcgg
     1921 gattctcctg ctcggctgca ccactggcca gggaccgggc agcctggtgg ataccgcgcc
     1981 ggatgcggac ataggtggca cagcggcaga tattgccg
//
LOCUS       DASUEG010000992         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1538880, whole genome shotgun sequence.
ACCESSION   DASUEG010000992 DASUEG010000000
VERSION     DASUEG010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538880"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFX11_18595"
     CDS             complement(<1..855)
                     /locus_tag="VFX11_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940690.1"
                     /translation="MDSGANFLRCGVFCNVAIAVPHFSNNNNLFIAKGHNVKTIKTLL
                     FLLTVIGLTACKQVNITAPTSNFVDDEKPTAFTLTFTGGAPTDLKIQLNSTIVTDRFA
                     VTATGATATGDQLADVVYPGRNFFRVTGNKMVKQITFYYDTEGPSIRILDTDHDAKTV
                     TGYVEDKGGIASVSLDGVAISLDAKNGFSVPFADQDVHTFVATDNFGHTSNTDFADRE
                     SEFAGISARLNQGGFDFLTSALRQAIEGKNLASSIKDNKFIVSDIRLGDACVVPCVSF
                     AIDNFSVSQ"
     gene            1191..1925
                     /locus_tag="VFX11_18600"
     CDS             1191..1925
                     /locus_tag="VFX11_18600"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX4940691.1"
                     /translation="MNTGTGGINAVIGDLSAVRAGIPVYRLGEQARIQHHLQCVRDYL
                     AANAPRDLTVSQRRNRRQVLSVLQTYIDQGVFPEHDDPAVEQRLPRFIDQRGVHCAVG
                     YLARETGDERWGEQINARFEYAFVPQIESPDLMDWAQVNGLSVRDCALIQPSYLPPDW
                     PLSECPMLFLARNTSLETRLDIVREFRDTQLVRAPGGRWAVKYYYQTGPQVVKFLHAH
                     RWSQLPARTFLALLIDLLEARRAGQH"
     gene            2025..2336
                     /locus_tag="VFX11_18605"
     CDS             2025..2336
                     /locus_tag="VFX11_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940692.1"
                     /translation="MLRKPGCRQILMLLALSPVAALADDICPDPVAFIDAGWQHESVQ
                     DAMDDYLRDHRMRANWGQEPVPPDAVGRDYYEWLWISEFGAKMKGELVQRFCGATQPA
                     E"
     gene            complement(2338..2964)
                     /locus_tag="VFX11_18610"
     CDS             complement(2338..2964)
                     /locus_tag="VFX11_18610"
                     /inference="COORDINATES: protein motif:HMM:NF039449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6436 domain-containing protein"
                     /protein_id="HEX4940693.1"
                     /translation="MDMPAPPPDSRSPTPPGARRWLYVIALIWLLLVAGLLWYSLRLP
                     QQWFDPLQTQPHAFQDASALHRLTAALATQHPDLKPGQPLFIRFRQTDCRCEQLVDAY
                     HQLQTKLLQQQGYRVLTVEKADLEQLARTQLPELFSWITATPAVMVVDGNGALAYFGP
                     YHQEGVCNSENSYLEPVLQAVNTQQPVNIINTLVFGCFCATAPSRGTN"
     gene            3067..3357
                     /locus_tag="VFX11_18615"
     CDS             3067..3357
                     /locus_tag="VFX11_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX4940694.1"
                     /translation="MPMNKTTEIEAAVFRRLLQHLDDHKEVQNIDLMNLAGFCRNCLA
                     KWMVTAAQEQGETLTYDQAREKIYGMPYDEWKQRYQKEASAGQLEQFNQRKD"
     gene            3373..>3772
                     /locus_tag="VFX11_18620"
     CDS             3373..>3772
                     /locus_tag="VFX11_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4940695.1"
                     /translation="MSSVLTCPLCNGQNIEPWHRDRRRDYLRCVDCELVFVPASFHLD
                     TAAEKAVYDQHENDPADAGYRRFLMRLAQPLLQRLPLQSTGLDFGCGPGPALAAILRE
                     AGHSVALYDKFYFPDAATLQQQYDFICATEV"
BASE COUNT          787 a         1008 c         1170 g          807 t
ORIGIN      
        1 ttggctaacg ctgaaattgt caatagcaaa cgatacgcaa ggtactacgc aagcgtctcc
       61 aagccttata tcagaaacaa tgaacttatt atctttaatg gaggaggcaa ggtttttacc
      121 ttcgattgcc tgtctcagcg cggaggtaag gaaatcaaac ccaccctgat tcaatcgcgc
      181 agaaataccc gcgaactcgc tctcccgatc cgcaaaatcc gtattgctgg tatgaccaaa
      241 attatcggtc gcaacaaacg tatgcacatc ctggtcagca aacggcacac taaacccatt
      301 cttcgcgtcc agcgaaatcg ccacaccatc cagactcaca ctcgcaatgc cgcctttgtc
      361 ttccacatag ccggtgacgg tcttggcgtc atggtcggta tccagaatgc ggatcgacgg
      421 gccttcggtg tcgtagtaga aggtaatctg tttcaccatc ttgttgccgg tgacgcggaa
      481 gaagttgcgg ccggggtaca cgacgtctgc cagctgatca ccggttgcag tggcaccggt
      541 ggcggttacg gcgaaacggt cagtaacgat ggtggagtta agctggattt tcaggtcggt
      601 cggagcgccg cccgtgaacg tcagcgtgaa agcagtgggc ttttcatcgt caacgaaatt
      661 gctggtggga gctgttatgt ttacctgctt acaggcggtg agcccgatta ctgtaagcaa
      721 aaacaacagt gtttttattg ttttcacgtt gtgccccttt gcgatgaaca aattgttgtt
      781 gtttgaaaaa tggggtactg caattgctac gttgcaaaaa actccacagc gcaaaaagtt
      841 ggcgccactg tccatgctac ttaattgatc actgcagtaa agttaaattt gtttgctgat
      901 tgcgactgat tagttaatca agcagcgact ggggcggcta tgccattgat tgttgtccgg
      961 agtggcactg agtgtaacgt taggtaacgc tgacggccaa tgtgaggcgc cataaactaa
     1021 tccctgtagg cgcgggcagg cattgccggc accccttctt ccctgccgct gaatcgtgtt
     1081 acaagggcgt tacggcagtc aatccggcgt ggatggtctg gagtgccggt gacagtcgta
     1141 gtctcgacaa cgacggttca aacaataata aacgtagaag gagctgcaac atgaacacag
     1201 gaacaggcgg catcaatgcc gtcatcggcg acctcagcgc cgtgcgcgcg ggcattccgg
     1261 tgtaccggct gggcgagcag gcgcggattc agcatcatct gcaatgtgtg cgcgattatc
     1321 tggcggccaa tgcgccgcgg gatttgaccg tttcccagcg ccgcaaccgg cggcaggtgt
     1381 tgtcggtgct gcaaacctat atcgaccagg gcgtttttcc cgaacatgat gatcctgcgg
     1441 tggagcagcg tctgccacgc ttcatcgatc agcgcggcgt gcattgtgca gtggggtatt
     1501 tggcgcggga aaccggcgat gaacgctggg gtgagcagat caatgcgcgc ttcgaatatg
     1561 cctttgtacc gcaaatcgaa agtccggatc tcatggactg ggcgcaggtg aatggcctga
     1621 gcgtgcggga ttgcgcgctg attcagccgt cctaccttcc gcccgactgg cccttgtctg
     1681 aatgtccgat gctgtttctg gccaggaata cgtcgctgga aacccggttg gatatcgtgc
     1741 gtgaatttcg cgatacgcag ttggttcggg caccgggtgg ccgctgggcg gtgaagtatt
     1801 attaccagac cggtccgcag gtagtgaaat ttctgcatgc gcaccgctgg agccagctgc
     1861 cggcgcgcac attcctggcg ctgctgatcg acctgcttga agcgcgcagg gccgggcagc
     1921 actgaacgtg ggagcggggt tcgccacgaa gtgtgcgggt gaaccctgcc ggcggctgga
     1981 atatccggcc cgggaaaaaa tccgtcatga aggaggagtg cgacatgttg cgtaaaccag
     2041 gttgcaggca aattctgatg ctgctggcac tgtcaccggt tgcagcgctg gctgacgaca
     2101 tctgcccgga cccggtggcg ttcatcgatg ctggctggca gcacgaatcc gtgcaggatg
     2161 cgatggacga ttatctgcgt gatcatcgca tgcgggccaa ctgggggcag gagccggtgc
     2221 caccggacgc cgtggggcgc gattattacg agtggctgtg gatcagcgaa tttggcgcaa
     2281 agatgaaagg cgaactggtg cagcgattct gcggcgccac gcagcctgcg gaatgattca
     2341 gttcgtgccg cgtgacggcg ccgtggcgca gaagcagcca aataccagcg tgttgatgat
     2401 gttcaccggt tgctgcgtat tcaccgcctg cagcaccggt tccagatagc tgttttcact
     2461 gttacagacg ccttcctgat gataggggcc gaaataggcc agcgcaccgt ttccatccac
     2521 caccatcacg gccggcgttg ctgtgatcca gctgaacagt tccggcagtt gcgtccttgc
     2581 cagttgctcc agatcggctt tttccacggt cagcacgcgg tatccctgct gttgcagtaa
     2641 cttggtttgc agttgatggt aggcgtccac cagctgctca cagcggcagt ccgtctggcg
     2701 gaaacggatg aacagcggct gcccgggctt cagatcggga tgctgcgttg ccagcgcggc
     2761 ggtgaggcgg tgcagagcgc tggcgtcctg gaaggcgtgg ggctgtgtct gcagcgggtc
     2821 gaaccactgc tggggcaggc gcagggaata ccacagcagg ccggccacca gcagcagcca
     2881 gatcagggcg atcacgtaca gccagcgccg ggcgccggga ggcgtgggcg agcggctgtc
     2941 ggggggcggg gcaggcatgt ccatgatgat gggtctgtgt ggcggcggtc agccggcatt
     3001 ctagcaggcg ggcgcgcgcg attggaaagg catgcggtag aatggattcc cgttttttac
     3061 cggtgcattc ccatgaacaa gaccactgaa atcgaagctg ctgtttttcg ccgcctgctg
     3121 caacatctgg atgaccacaa ggaagtgcag aatatcgacc tgatgaacct ggcgggtttc
     3181 tgccgcaact gtctggccaa gtggatggtg acggccgcgc aggagcaggg cgagacgctc
     3241 acctatgatc aggcgcggga aaaaatctac ggcatgccct atgacgagtg gaagcagcgc
     3301 taccagaagg aagccagtgc cggacaactg gaacagttca accagcgcaa ggactgagag
     3361 cgactgccgg ccatgtcatc tgtgctgacc tgtcctctgt gcaacgggca aaacattgag
     3421 ccctggcacc gcgaccgccg ccgtgattac ctgcgttgcg tggactgcga actggtgttc
     3481 gtgccggcat cgtttcatct ggataccgct gcggaaaaag ccgtgtacga ccagcatgaa
     3541 aacgatcccg ccgatgcggg ttaccggcgt tttctgatgc gactggcgca gcctttgctg
     3601 cagcgcttgc cgctgcaatc caccggactg gatttcggtt gcggccccgg cccggcgctg
     3661 gcagcgatat tgcgggaggc cgggcattcc gtcgcgctgt acgacaagtt ttattttccc
     3721 gacgcggcga cgttgcagca gcaatacgat tttatctgcg ccaccgaagt gc
//
LOCUS       DASUEG010000993         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1914216, whole genome shotgun sequence.
ACCESSION   DASUEG010000993 DASUEG010000000
VERSION     DASUEG010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914216"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /gene="priA"
                     /locus_tag="VFX11_18625"
     CDS             complement(<1..1643)
                     /gene="priA"
                     /locus_tag="VFX11_18625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4940696.1"
                     /translation="MTFSAVKPTSPTPRPAPAHTIVQVALPVPVRRTFDYLPRADQPV
                     TDLQPGRRVQVPFGKKQVIGIIAGVVPHSAYGDDKLKTLTALLDDEPALTPPILRLCQ
                     WATDYYQHAAGDVLGAALPNQLRNSPDSWDERAWRWQLTQKGRLIGLEQLGRAKRQQD
                     AVRLLREHPDGLHQPILTGFGVQPAILRALADKGLIEQHEDGPARAHWRREQILAESP
                     LTLNDEQKHALEPLNACAEFRVFLLEGVTGSGKTEIYLQAITHCLQQGRQALVLVPEI
                     GLTPQTLHRFQQRFAVPVVSFHSHLTDRERLASWRKARTGAAAILIGTRSALFTPLEN
                     LGLIIIDEEHDLSYKQQEGFRYNARDLAIMRGKIENVPVLLGSATPSLESLHNARSGR
                     YVHLALTQRAGNAQQPRFRVLDIRQKPLTHGMAPEMLEAMQQTLQAGRQVLVFINRRG
                     YAPVLMCHDCGWFKECPHCDHRMTCHANPAHLHCHHCNHQERIPRHCGHCKSTDLRPI
                     GMGTERLEDNLTALFPKFPVIRIDRDTTQRKQAMAQHLER"
     gene            1820..2038
                     /gene="rpmE"
                     /locus_tag="VFX11_18630"
     CDS             1820..2038
                     /gene="rpmE"
                     /locus_tag="VFX11_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX4940697.1"
                     /translation="MKADIHPQYGEMTATCTCGNVIATRSTLSKDIHIDVCSACHPFY
                     TGQQKVVDTGGRIDKFKKRFGAVASIKK"
     gene            2089..2949
                     /locus_tag="VFX11_18635"
     CDS             2089..2949
                     /locus_tag="VFX11_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX4940698.1"
                     /translation="MGAVYGWSLLWLALLAAPVRAAPDCALADSVKTESVQAEAVLDG
                     DTLVLTDGRRVRLIGVNTPEVEHPPRPAEPLADDALRQLQQLAGKSPLYLQVGAEPRD
                     HYGRTLGHLYAADGRNVIAELLRQGLGFQVAIPPNLDHADCYAAAQAQARQVGAGVWS
                     HDYFRPQPADSDRLKGGYQRIQGRVEKVSLTKKVIWVDLAGNVSLKLDRRHAAYLEGV
                     TLDQVVAQSRAGPGRGALVLEVRGWLIDRSRWGGRMQQQIASGQRKRFMVNLSHFSQW
                     ETLPVSAQSR"
     gene            3225..4496
                     /locus_tag="VFX11_18640"
     CDS             3225..4496
                     /locus_tag="VFX11_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX4940699.1"
                     /translation="MSSDLKKAALEYHALPRPGKISVELTKPTETARDLSLAYSPGVA
                     EPVREIAKDPEAAYKYTNKGNLVAVISDGTAILGLGDLGCLASKPVMEGKGVLFKRFA
                     GIDVFDIEVDAESPQAFIDTVKRISPTFGGINLEDIKAPECFEIERVLIEQCNIPVFH
                     DDQHGTAIIAAAALINALEIQHKRIEDISIVCIGAGAAGVATLRLLVNMGAKKTNMYV
                     LDRNGVIHSGRSDLNQYKAAFAHETDKRTLEDALAGADVFIGVSSANLLTPDMLRLMA
                     DNPIVFALANPDPEIRPELAYATRSDVIVATGRSDYPNQVNNVLGFPYIFRGALDVRA
                     TRINEEMKVAAVRAISRLAKEPVPEVVEAAYEGQHLEFGREYIIPKPMDPRLLGAVSA
                     AVARAAVDSGVATRPYPAHYPLETQADIRYY"
BASE COUNT          878 a         1449 c         1482 g          893 t
ORIGIN      
        1 cgttccagat gctgcgccat cgcctgcttg cgctgggtgg tgtcgcgatc gatgcggatc
       61 acgggaaatt tcgggaacag ggcggtgaga ttgtcctcca gccgctcagt gcccatgccg
      121 atggggcgca gatcggtgct cttgcagtgg ccgcagtggc ggggaatccg ttcctggtga
      181 ttgcagtgat ggcaatgcag gtgggccgga ttggcgtggc aggtcatgcg gtggtcgcag
      241 tgggggcatt ccttgaacca gccgcagtcg tgacacatca gcaccggtgc atagccgcgc
      301 cggttgatga acaccagcac ctggcgaccc gcctgcagag tctgctgcat ggcttccagc
      361 atttccggcg ccataccgtg ggtgagcggt ttctggcgga tatccagcac ccggaaacgc
      421 ggttgctggg cattgccggc gcgctgggtc agcgccagat gcacataacg gccgctgcgg
      481 gcgttgtgca gggattccag gctgggagtg gcactgccca gcagcaccgg cacgttctcg
      541 atcttgccgc gcatgatcgc cagatcgcgg gcgttgtagc ggaatccctc ctgctgttta
      601 taggaaaggt cgtgttcctc atcgatgatg atcaggccca ggttttccag tggtgtgaat
      661 agcgccgagc gggtgccgat caggatcgcg gccgcgcccg tgcgcgcctt gcgccagctg
      721 gccagtcgct cccggtcggt gaggtgggaa tggaagctca ccaccggcac cgcgaaccgt
      781 tgctggaaac ggtgcagagt ctgcggcgtc aggccgattt ccggcaccag caccagcgcc
      841 tgcctgccct gctgcaggca atgcgtgatg gcttgcaggt aaatttccgt cttgccgctg
      901 ccagtcacac cctccagcaa aaatacgcgg aattcggcgc aggcattgag cggctccagc
      961 gcatgctttt gttcgtcgtt cagcgtgagg gggctttccg ccagaatctg ctcgcgccgc
     1021 cagtgggcgc gggcggggcc atcctcgtgc tgctcgatca ggcctttgtc cgccagagcc
     1081 cgcagtattg ccggttgcac gccgaacccg gtcagtatcg gctggtgcag gccatccgga
     1141 tgttcccgca gcaggcgcac cgcatcctgc tggcgcttgg cccggcccag ttgctccagc
     1201 ccgatcaggc gacctttctg cgtcagctgc cagcgccagg cccgctcgtc ccaactgtcg
     1261 ggactgttgc gtagctggtt gggcagggcg gcacccagca cgtcgccggc ggcatgctgg
     1321 taatagtcgg tggcccactg acacaggcgc aggatcggcg gggtgagcgc aggctcgtca
     1381 tccagcaggg cggtaagggt tttcagcttg tcgtcgccat aggcggaatg gggtacaacg
     1441 ccggcgataa tgccgatcac ctgcttcttg ccgaagggta cctgcacccg ccggccgggc
     1501 tgcaaatctg tcaccggctg gtcggcgcgg ggcagataat cgaacgtgcg ccgcaccggc
     1561 acgggcagcg ccacctggac aatggtgtgg gccggagcag ggcggggcgt gggggaagtc
     1621 ggcttcacgg cggaaaaagt caatgcaatc cagatggttg cgggatcggg gttgcggcca
     1681 ctctactgtg tcggccaaca atttgccaga ctttggcaac ctctggtatc atgcgcgccc
     1741 ttgtgacata cccatgtacc cttcatgcgg tgcccggccc agaccgggtg gcggcgtgaa
     1801 cccgaaaggt gagacttcca tgaaagctga catccatccc caatacggcg aaatgactgc
     1861 aacctgcacc tgcggcaacg tgatcgccac ccgctccacc ctgagcaaag acatccacat
     1921 cgacgtctgt tccgcttgcc atcccttcta cactggccag cagaaagtgg tcgataccgg
     1981 tggccgcatc gacaagttca agaagcgttt cggcgcggtg gcttccatca agaaataatg
     2041 cgcgtgtatt cccgcagcag cagaaaggct ccccgtggag ccttttttgt gggcgccgtt
     2101 tacggatgga gcctgctctg gctggcgctg cttgccgcac cggtgcgggc agcgccggat
     2161 tgtgccctcg ccgattccgt caagactgaa agcgtgcagg cggaagcggt actggacggc
     2221 gacactctcg ttcttacgga cgggcgccgg gtgcggctga tcggggtgaa cacgccggaa
     2281 gtggagcatc caccacgtcc cgccgagccc ctggcggacg acgccctgcg ccagttgcag
     2341 caactggctg gaaaaagccc gctctacctg caggttggcg ccgagccccg cgaccattac
     2401 ggtcgcacgc tgggtcatct gtacgcggcg gacgggcgca acgtcattgc cgaattgctc
     2461 cggcagggac tcggattcca ggtggcgatt ccgcccaacc ttgaccacgc cgattgttac
     2521 gccgccgccc aggcacaggc ccggcaggtg ggcgcgggtg tttggagcca tgattatttc
     2581 aggccacaac ctgccgacag cgacaggctc aagggcggtt accagcgcat acaggggcgg
     2641 gttgaaaagg tgtccctcac caaaaaggtg atctgggtcg atctggcagg caatgtcagt
     2701 ctgaaactgg accgccgtca tgcagcgtat cttgaaggtg tgacactgga tcaggttgta
     2761 gcccagtccc gcgccggccc tggcagaggt gccctggtgc tggaagtgcg cggctggctg
     2821 atcgatcgca gccgttgggg tggcaggatg cagcagcaaa ttgcgtccgg ccagcgcaag
     2881 cgtttcatgg tcaacctgtc ccatttcagc cagtgggaaa cactgccggt gtccgctcag
     2941 tcacgctgaa gcgttttctt acaaccggag tctccacttc tgcgccgacc ccgcattttc
     3001 ctgcgtgaca gcatgaatcg ggtgttgagc accccgagtg atttcgctat cctacaccac
     3061 cccctttttt cgaccgcgcc gggtggatta aacctgtcca ttcaggctcg tgcgtttcgc
     3121 cagttgacgt gcaccactgc tggccagcca caaccaggct ctgaacactc aatgattcaa
     3181 taagcagacc atcccagtat gccccagtag ggaaccctat atatatgtcg tccgatctga
     3241 agaaagccgc gctcgaatat catgccttgc cccgtccggg caagatcagt gtcgagctca
     3301 ccaagcccac cgagactgcc cgcgatctgt ccctggccta cagcccgggt gtggccgagc
     3361 cggtgcggga aatcgccaag gatccggaag cagcgtacaa gtacaccaac aagggcaacc
     3421 tggtggcggt catcagcgat ggcaccgcga ttctgggtct gggtgatctg ggttgcctgg
     3481 cgagcaagcc ggtcatggaa ggcaagggcg tcctgttcaa acgtttcgcc ggcattgacg
     3541 tgttcgacat cgaagtggac gcggaaagcc ctcaggcctt tatcgacacg gtgaagcgca
     3601 tctcgcccac cttcggcggc atcaacctgg aagacatcaa ggcgccggaa tgcttcgaga
     3661 tcgagcgcgt gctgatcgag cagtgcaata ttccggtgtt ccatgacgac cagcacggca
     3721 cggccatcat cgccgccgcc gccctgatca acgcgctgga aattcagcac aagcgcatcg
     3781 aggatatctc catcgtctgc atcggtgccg gtgcggcggg tgttgcgacc ctgcgcctgc
     3841 tggtgaatat gggggcgaag aaaaccaaca tgtatgtgct ggaccgcaat ggcgtgatcc
     3901 attccggccg cagcgacctg aaccagtaca aggcggcatt tgcccacgaa accgacaagc
     3961 ggactctgga agatgcgctg gcaggggcgg acgtgttcat cggtgtgtcc agcgccaatc
     4021 tgctcactcc ggacatgctg cgcctgatgg cagacaatcc tatcgtgttt gcgctggcga
     4081 atcccgatcc ggaaatccgc ccggaactgg cctatgccac ccgcagcgac gtcatcgttg
     4141 ccaccggccg cagcgattac ccgaaccagg tcaacaacgt actgggtttc ccctacattt
     4201 tccgcggcgc cctggatgtg cgtgccacgc gcatcaacga ggaaatgaaa gtggcggcgg
     4261 tgcgcgccat cagccgtctt gccaaggagc cggtgcctga agtggtggaa gcggcctacg
     4321 aaggacagca cctggaattc ggccgcgagt acatcattcc caagccgatg gatccccgct
     4381 tgctgggcgc cgtgtcggcg gcagtggcgc gggcggcggt ggattccggc gtggcgaccc
     4441 gtccctaccc cgcacattat ccgctggaga cgcaggcaga catccgctac tactgatgtc
     4501 tgctcacccg ggcgggaaac attttccaca gtcaataaaa aagccgactc cgtacagtcg
     4561 gcttttttgt gtctgcaagt gcgggcgcgt cagatagtgt taacgggccc tagaacagcg
     4621 attcgggcat ttcaccgctg gccccaccgc ttccggcaga ggcgggccct tcagcgcctt
     4681 ccacaataaa ggccggcaca tc
//
LOCUS       DASUEG010000994         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_725179, whole genome shotgun sequence.
ACCESSION   DASUEG010000994 DASUEG010000000
VERSION     DASUEG010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725179"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFX11_18645"
     CDS             <1..900
                     /locus_tag="VFX11_18645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940700.1"
                     /translation="GNDWARYLLLGWSTTITGYCMFILAMLDLLEFNGTVLFLQAAGL
                     GLGNVMVTTAIAARVRRERREKASAIREALQLSREVAQLNREKEQLQQYASQQLQQEV
                     EEKTRTLNQKLQNLQNSNRQLERDSLSDALTGLGNRRYLDAVFPESIRQCSQHRAPLG
                     ILVIDADHFKRVNDTFGHLAGDECLRKLSTILQKYCRRNLDTLVRYGGEEFVMLLPAT
                     DLEGVLKVAESIRHHVEHAQFWFDNQRVPVTVSLGVYVGIPAPQASAERLMHKADEAL
                     YLAKRNGRNRVEIYKAALESVKA"
     gene            1066..1923
                     /locus_tag="VFX11_18650"
     CDS             1066..1923
                     /locus_tag="VFX11_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940701.1"
                     /translation="MRWNRLLLLMLAPALLTACGGGDSDDQDYEDSVSVPYGVIFQVN
                     GVQDATAVTIQQVELNADNGYAEDCDTDNADDLVGETEYCDDVRNIGIVTFQFRFINN
                     LLRSIPFSFSGVGVTVQIERQTGTDPETWELVWDSDAYLRVQYKIFGLAEYDPNALGL
                     NNVTGAPYTSLAPAEAYPNQDYAALTFRFLTERNFIDDNGNGKFDQDEDRLVALPGTT
                     TAGNLEGSDKCAWVPTLNEGTNKKNMPACQTEELPAGTYRATAHYHFAIDGELADPPP
                     VIITVNPAQ"
     gene            1959..>2826
                     /locus_tag="VFX11_18655"
     CDS             1959..>2826
                     /locus_tag="VFX11_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX4940702.1"
                     /translation="MHEPSRPGLTANQAREALEHHGPNALPEQPAESFLHRYLRQFRN
                     PVIYILLFALAFDLAIWWQEGADGIPLEAVTILVILLFNSILGAWQETKSEAALARLK
                     ALAAPRTWVLRDGCLVQLPSVELVPGDLIRIEAGERIPADGTLESANSFSADESILTG
                     ESVPLEKGIGDLVLSGTLAVRGLGWIRIAATGQQSTMGKLAHLLQSVEREQTPLERRL
                     AKFGRQVAICVIGLAALVLLAGMSTQGFDDINRLLLFAVALAVAAVPESLPAVLTLAM
                     ALGMERMAARKAV"
BASE COUNT          577 a          873 c          842 g          534 t
ORIGIN      
        1 ggcaatgact gggcccgcta tctgttgctg ggctggtcca ccaccatcac cggctactgc
       61 atgtttatcc tggcgatgct ggacctgctg gaattcaacg gcaccgtcct gttcctgcag
      121 gcggcggggc tgggactggg caacgtcatg gtcaccacag ccattgccgc acgggtgcgg
      181 cgtgaacggc gtgaaaaggc atctgccatc cgcgaggcat tgcagctgag ccgtgaagtg
      241 gcacagctca atcgtgaaaa ggagcagctg cagcagtacg cctcacagca gttgcagcag
      301 gaagtggagg aaaaaacccg cacgctcaac cagaagctgc aaaacctgca aaactccaac
      361 cgccagctgg aacgggattc cctctcggat gcgctcaccg gcctgggcaa ccggcgttac
      421 ctggatgccg tgtttcccga gtccatccgg cagtgttccc aacaccgtgc acctttgggc
      481 atcctggtca tcgacgccga ccacttcaag cgcgtcaacg acaccttcgg ccatctggca
      541 ggggacgaat gcctgcgcaa gttgtccacc attctgcaga agtactgccg tcgcaacctg
      601 gacaccctgg tgcgctacgg tggcgaggaa ttcgtcatgc tgctgccggc cacagacctg
      661 gagggggtgc tgaaggtggc agagagcatc cgtcatcatg tcgagcacgc ccagttctgg
      721 ttcgacaacc agcgcgtgcc agtgacggtc agccttggcg tgtacgtcgg cattccggcc
      781 ccccaggcca gcgccgaaag actgatgcac aaggccgacg aagccctgta cctggccaaa
      841 cgcaacggcc gcaaccgggt ggaaatctac aaggccgcac tggaaagcgt gaaggcctga
      901 accggcccga tcatccgcag accgaacaaa gccacaactc cagggtccat actgctgcat
      961 cccgatcgcc tgccggcggt tgagttttgc ctgcggggct gatttaatgc caagcccatt
     1021 gttggggcga accgggactg acctggatac cttgtggaga aaagaatgcg ttggaacagg
     1081 ctcttgttgc tcatgctggc accggcactg ctgactgcgt gtggtggagg ggatagcgac
     1141 gatcaggact acgaggacag cgtgtcagtg ccgtacggcg tcattttcca ggtcaacggg
     1201 gtgcaggacg ccacggcggt cacgattcag caggtcgaac tgaatgctga caatggctat
     1261 gctgaagact gcgacacaga caacgcggac gatctggtcg gcgagacaga gtactgcgat
     1321 gacgtgcgga atatcggcat cgtcaccttc cagttccgct tcatcaacaa cctgctgcgc
     1381 tccattccct tttccttcag cggtgtcggc gttactgtgc agattgaacg ccagacgggc
     1441 accgatcccg agacgtggga gctggtttgg gattccgatg cctatctccg cgtccagtac
     1501 aagatctttg gcctggcaga gtacgatccg aatgctctcg gcctgaataa tgttacagga
     1561 gccccctaca cgtcactggc gcctgcggaa gcctacccca accaggatta tgcggctttg
     1621 acgttcaggt ttctgacaga gcgcaatttc attgatgaca atggcaacgg caagttcgac
     1681 caggacgagg acaggctggt ggctttgccc ggaaccacca ctgccggtaa ccttgagggc
     1741 tccgataaat gcgcatgggt accgacgctg aacgaaggga cgaacaagaa aaacatgccg
     1801 gcctgccaga ccgaagaact gccggccggc acctaccgtg ccactgccca ctaccatttc
     1861 gcaatcgatg gtgagctggc ggatccgcct ccggtcatca tcaccgtcaa tcccgctcaa
     1921 tgatgtccaa ttgagcgccc gagcccgagt gcaataccat gcacgaacct tcccggcccg
     1981 gcctcactgc taatcaggcc cgcgaggcgt tggagcacca tggccccaac gcattgccgg
     2041 agcaaccggc cgaatccttc ctgcaccggt atctccgcca gttccgcaac cccgtcatct
     2101 acatcctgct gtttgccctg gccttcgatc tggccatctg gtggcaggaa ggtgcagatg
     2161 gcattccgct ggaagctgtc accatcctgg tgatcctcct gttcaactcc attctgggtg
     2221 cctggcagga aacaaagtcc gaagccgcac tggcccgcct aaaagcgctc gccgcgccca
     2281 gaacctgggt gttgcgcgat ggatgtctgg tgcagctccc cagcgttgag ctggtgccgg
     2341 gcgacctgat ccggatcgag gcgggcgagc gcattccggc cgatggtacc ctcgaatcag
     2401 ccaacagctt ttccgctgac gaatccattc tcacgggcga atcggtaccg ctggaaaaag
     2461 gcatcggcga cctggtgctg tccggcactc ttgccgtgcg ggggctgggc tggatccgaa
     2521 ttgcagccac cggtcagcag tccaccatgg gcaagctcgc tcacctgttg cagtccgtgg
     2581 agcgggaaca gacaccactg gagcgacggc tggcaaaatt cggccggcag gttgccatct
     2641 gcgtgatcgg gttggctgca ttggtcctgc tggcgggcat gtcaacccag ggatttgatg
     2701 acatcaaccg gcttctgctg tttgccgtag cgttggcggt ggccgctgtc ccggaaagcc
     2761 tgcccgccgt cctcaccctg gccatggccc tgggcatgga gcgcatggcc gcccgcaaag
     2821 cggtgg
//
LOCUS       DASUEG010000995         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_324109, whole genome shotgun sequence.
ACCESSION   DASUEG010000995 DASUEG010000000
VERSION     DASUEG010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324109"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFX11_18660"
     CDS             <1..551
                     /locus_tag="VFX11_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX4940703.1"
                     /translation="TSQGALPFDLCLCAPGFKASPLVASMTDQLDDNGRAKVTDTLQL
                     VGDDRIWIAGDSAAFPWQGPGPLRMGCAVALPMGAQAGENVAAVIKGNEPTPFSFGFL
                     IRCVSLGRKAGVVQTTQWDDKARSRVFTGATGAWIKERVCRMTFNIPRWELATGLRMY
                     RWPPVPAIHQDIQRTVIDSVEG"
     gene            555..1445
                     /gene="sigJ"
                     /locus_tag="VFX11_18665"
     CDS             555..1445
                     /gene="sigJ"
                     /locus_tag="VFX11_18665"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX4940704.1"
                     /translation="MENNTAIFERERGRLFGLAYRMLGSVADAEDVLQEVCLRWLQQD
                     SDTIANPAGWLLTVVTRECIDWQRSARHRREHYVGVWLPEPLLESFHDTVDGPQAMTE
                     LANNLTLAFLHLLEKLNPLERAVLLLRQAFDWPFSDIAATLGKAEAHCRQLFKRAQEK
                     IGSDIAMTRPDANAERLLLQFMQTCTSGDFSGLLNCLHEDLIAYSDGGGKVRSLLRPI
                     AGSERVARFLRRIIQPRANLAVIARMINGEPGLLAFENGQPVAALIFAFREQKLWRLY
                     SIRNPDKLTGALFDQQSGEQ"
     gene            complement(1501..1896)
                     /locus_tag="VFX11_18670"
     CDS             complement(1501..1896)
                     /locus_tag="VFX11_18670"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEX4940705.1"
                     /translation="MRSLSWFAFLAFASGTLLIAHPAIAGSMRCGRHIINEGASQYEV
                     HAKCGDPVYQQLIEEPVTVISTSQASIYSRLGANGQNLQQDILLQEEQPMYRTIERWT
                     YNFGSGRLLREVDFFNGDVIRIETGSRAP"
     gene            complement(1997..>2473)
                     /locus_tag="VFX11_18675"
     CDS             complement(1997..>2473)
                     /locus_tag="VFX11_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011041569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HEX4940706.1"
                     /translation="IGLYRRVAVYEEFVVGAKEGFETAVRLIPYLVAMLVAIAVFRAS
                     GALDFVGRAMSRVCEAVGLDTAFVDALPVALMKPFSGSGARAMMLDVMNNHGVDSFVG
                     KVAAVMQGSTETTFYVLTVYFGVVGITRIRYALWCGLLCDVVGLAMAVLVSYWFFG"
BASE COUNT          542 a          725 c          716 g          490 t
ORIGIN      
        1 gtacttcgca aggcgcattg ccctttgacc tgtgtttatg cgcgccggga ttcaaggcat
       61 caccgctggt agccagcatg accgatcagc tggatgataa cggacgggcc aaggtcaccg
      121 acacattgca actggtcggc gatgaccgga tctggattgc cggggacagc gctgcatttc
      181 cgtggcaagg ccccgggccg ttacgcatgg gttgcgctgt cgccctgcca atgggcgcac
      241 aggcggggga aaatgtggct gccgtgataa aaggaaatga acctacgccg ttttcgtttg
      301 gttttttgat acggtgtgtc agcctgggca ggaaggcggg cgtggtgcaa accacacagt
      361 gggacgacaa agccagaagc cgggtattca ccggcgcgac aggtgcgtgg atcaaggagc
      421 gcgtgtgtcg catgaccttc aacattccgc gctgggaatt ggcgacgggc ctgcggatgt
      481 atcgctggcc accggtgcct gccatccatc aggatattca gcgtactgtc attgattctg
      541 tagaaggtta acgaatggaa aataacacgg caatcttcga gcgtgagcgc ggacggttgt
      601 ttggacttgc ctatcgcatg ctgggttcag tcgccgatgc cgaagatgtg ctgcaggagg
      661 tttgtctgcg ctggttgcag caggacagcg acaccatcgc caatcctgct ggctggctgc
      721 tcacagtggt aacgcgcgag tgtatcgact ggcagcgcag tgcccgtcat cgccgcgaac
      781 attatgtggg tgtgtggctt ccggaaccgc tgctggaatc ctttcacgac accgttgacg
      841 ggcctcaggc aatgactgaa ctcgcaaaca atctgacgct tgcgttcctg cacctgctgg
      901 aaaaactgaa tccgctggaa cgggcagtac tgctgctgcg gcaggccttc gactggccgt
      961 tcagtgatat cgccgcaacg ctcggaaaag ccgaagcgca ttgccgtcag ctgttcaagc
     1021 gcgcgcaaga aaaaatcggc agcgacatcg caatgaccag acccgacgcc aatgcggaac
     1081 ggttgttgct gcaattcatg cagacctgca ccagcgggga tttttcaggc ctgctcaact
     1141 gcctgcatga agacctgatt gcctactcgg atggtggtgg caaggtcagg tcgttgctgc
     1201 ggcccattgc gggcagcgaa cgcgtcgccc ggtttctgcg acgcatcatt caaccacgtg
     1261 ccaatctcgc agttattgcc cgcatgatca atggcgagcc tggattgctg gcattcgaaa
     1321 atggtcagcc tgtagcagcg ctgatctttg cgttcaggga gcaaaagttg tggcgtttat
     1381 actcgatccg caatcccgac aaattgacgg gcgcattgtt cgatcagcag tctggtgagc
     1441 agtgaaattt ctgtatccgc agcgacagat tcatatccgg ccggagcgtt gcgtttcgcg
     1501 tcagggcgcc cggctgccgg tttcgatccg gattacatcg ccgttgaaaa aatcaacctc
     1561 ccgcagcagg cgccccgagc cgaagttgta ggtccagcgc tcgatggtgc ggtacatggg
     1621 ctgctcttcc tgcagcagga tgtcctgctg caggttctgt ccgtttgcac ccaagcggct
     1681 gtagatactg gcctgggaag tggaaatgac cgtcaccggt tcctcgatca gttgctgata
     1741 aaccggatcg ccgcacttgg catgcacttc gtactggctg gcgccttcgt tgatgatatg
     1801 gcgaccgcag cgcatggagc cggcaatcgc cggatgcgcg atcagcaagg taccgctggc
     1861 aaaggcgaga aaggcaaacc acgaaaggga acgcataaaa cctcctgaaa agtgccgggc
     1921 ttggatttca gggtggtccg gccaaccatt cagtcatgct aacaatactt cctgtacgcc
     1981 cgctgaacgg cggacttcag ccgaaaaacc agtagcttac cagcaccgcc atggccagcc
     2041 ccacgacgtc gcacaacagt ccgcaccaga gcgcataacg aatgcgggta atgcccacca
     2101 cgccgaaata cacggtcagc acatagaagg tcgtctcggt ggaaccctgc atcacggctg
     2161 ccaccttgcc aacgaaggaa tccacgccgt gattgttcat cacgtccagc atcatggcgc
     2221 gggcaccact gccgctgaac ggtttcatca gcgccacggg cagcgcatcc acgaaagccg
     2281 tatccaggcc cactgcttcg cacacccggc tcatagcccg cccgacgaaa tccagcgcgc
     2341 cagacgcccg gaacaccgca atcgccacca gcatggccac cagataggga atcagccgca
     2401 ccgccgtttc aaaaccttcc ttcgcgccga caacaaattc ctcatacacc gccaccctgc
     2461 ggtacagacc aat
//
LOCUS       DASUEG010000996         5723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_628058, whole genome shotgun sequence.
ACCESSION   DASUEG010000996 DASUEG010000000
VERSION     DASUEG010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628058"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFX11_18680"
     CDS             <1..854
                     /locus_tag="VFX11_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940707.1"
                     /translation="CTYRKGQLVSSESVLAAKSPVPIATAASAGAPLAAAMATTTLVA
                     SSVSAPATRNDTARTVAAKPVPAAKATASAPVTRTAAPKPAVKPVPAPEIATVPPAIV
                     VAAAPVAAHASAATSSHVKSAIPASANETSFASKLEQEKQNLKKTSVAELNQTRSDIY
                     FTDNWRDQDLMALPEKAWWNKKSSLFKDELEVVSQHGDVRIRMLVSDYKGPGQYRLDS
                     VIIETPGQHFEKAGNGGTLVVLDDQQGWLSGSFNFEVNGQNGETLAFNDGLFRVKDQS
                     QLLSIMK"
     gene            complement(966..2954)
                     /locus_tag="VFX11_18685"
     CDS             complement(966..2954)
                     /locus_tag="VFX11_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940708.1"
                     /translation="MTYFVTGASGFIGRFLLEKLLKRQGTIYVLMREASLGKLDTLKA
                     LVGDTEGRIVPVIGDITQPKLGISADDMAMLKGKVKHFFHLAAIYDLSAPEAVQVSAN
                     IDGTRHAVKAAKEMAAGCFHHVSSIAAAGMYPGIFREDMFEEAEKLNDPYLRTKHDSE
                     AIVRKDCKIPFRIYRPAMVVGHSKTGEIDKIDGPYYFFGLIKKLRKLLPPWMPVIGIE
                     GGRFNIVPVDYVVDAMDYLAHKDGLDGKCFHLTDVDHYKIGEVINIFARAGHAPQMTM
                     RIDTRMFSFMPPFLRQTLGTLPPVKRITNTVLEDLGIPRSLLKFTQYHTKFDNRDARA
                     ALDGSGISCPRLDTYAWRLWDYWERNLDPELFIDRTLEGRVKGKVVVVTGASSGIGKA
                     SALKLAAAGAKVILAARTPEKLEETKEEITAAGGEAFTYSVDIANMEDCDRFAQQVLA
                     DFGRVDVLINNAGRSIRRSISLSYDRFHDFERTMQLNYFGALRLMMNFLPVMEKNRSG
                     HVINISSIGALTYPARFSAYVASKSALDAFSRCAASEYSDCNVHFTTINMPLVRTPMI
                     APTKIYDNVPTISPEEAADMVGEALIYRSKRIATKLGVFGQVVHAVAPKFAEIIINTG
                     FRMFPDSAAAQGKKDDSQPSVVSPEQMAFAYIMRGVHW"
     gene            complement(3005..4375)
                     /locus_tag="VFX11_18690"
     CDS             complement(3005..4375)
                     /locus_tag="VFX11_18690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX4940709.1"
                     /translation="MRTSNHRALEPMSVVDTAWLRMDSPDNLMVINGLLMFDEPMSYE
                     DFKEILEDRLIRRHKRFRQRPVQVGNQYLWEELPQVDMQYHLSALEGLQECCNEAEIR
                     AAVSRQIPAPLDADKPLWRFFFVPKYKEGTAVMVKVHHSYADGIALISVLDSIADKSV
                     LHSSPAANIKFPQRKPRSDALLHRAEFALTSAAFYTGFSVAWLFEAMRVILARPDSKT
                     AYKRKLTAHKQVGWAPSLDINEVKRVSKAMGCTINDVILGCVAGSLRRYLERIGEPVN
                     GVQLRATVPVNLRPIEDALQLGNRFGLVYLNLPVGEPTVERRIQAVRQAMRQLKSGVQ
                     AQMSYNVLGILGFFPRAVEKFFLDFFSSKASAVMTNVPGPAEPVYLKGRKLAKPMFWV
                     PQSGGIGIGVSILSYDNKVEFGFVADAALIPDPQAMIDDFVDEFASIKNAVLGVPVFS
                     GQLSHV"
     gene            4716..5366
                     /gene="fabR"
                     /locus_tag="VFX11_18695"
     CDS             4716..5366
                     /gene="fabR"
                     /locus_tag="VFX11_18695"
                     /inference="COORDINATES: protein motif:HMM:NF008402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HEX4940710.1"
                     /translation="MTEPLTRAEQKQQTRETIMTAAVRKMNDDRGFSSLSLREVAKEA
                     GIAPASFYRHFENMEDLALALVKEAGDALLKIMSVTRESEEKGENILENAVAMCMAHF
                     RENGPLFRVLARESTGNSPIIRRAIQRVLNELNKQLAAVIELEMKKRGRAITDAAWIA
                     EAIITIIFYIGVSNIDLSYARQKEAEKRLVHHVRAIYWGAEAMASSQHLERQLMLS"
     gene            complement(5376..>5723)
                     /locus_tag="VFX11_18700"
     CDS             complement(5376..>5723)
                     /locus_tag="VFX11_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4940711.1"
                     /translation="ALPFADADFDTVVACLVFCTIPEPENAAREAFRVLKPGGRLVFF
                     EHVASPRDTTRAWQNRLNPLWRKMACGCEINRDTRALFEAAGFRYESIDDFIHPKVPA
                     LVAPVIAGIAIKA"
BASE COUNT         1279 a         1763 c         1574 g         1107 t
ORIGIN      
        1 cctgcaccta ccggaaagga caactggtga gcagcgaatc cgttctggca gcgaaatcac
       61 ctgtgccgat cgccacagcc gcatccgcgg gtgctcccct ggcggctgcc atggccacta
      121 ccacccttgt ggccagcagt gtgtctgctc cggcaacccg caacgacact gcccgcactg
      181 tcgccgcgaa accggtgccc gccgcaaagg cgaccgccag cgcccccgtg accagaacag
      241 cagccccgaa gccggctgta aaaccggtac cggcgccgga gatcgcaacc gtcccaccag
      301 ccattgtggt ggcagcggcg ccggtggcgg cccatgcctc ggctgccact tccagccatg
      361 tcaaatctgc cattccggca tcggcgaacg aaaccagctt cgccagcaaa ctggaacagg
      421 aaaaacagaa cctgaaaaag acctcggtgg cggaactgaa ccagacccgc agcgacattt
      481 acttcaccga caactggcgt gaccaggacc tgatggcgtt gccggaaaaa gcctggtgga
      541 acaagaaatc ctcgctgttc aaggatgaac tggaagtcgt gtcccagcat ggcgatgtgc
      601 gcatccgcat gctggtcagt gattacaagg gaccgggcca gtaccgtctc gacagcgtga
      661 tcatcgaaac acccgggcag cacttcgaga aagccggcaa tggtggcacc ctcgtagtac
      721 tcgacgacca gcagggctgg ctgagcggtt ccttcaattt cgaagtgaac gggcagaacg
      781 gagaaaccct ggcgttcaac gacggcctgt tccgggtgaa agaccagagt cagctgctgt
      841 cgatcatgaa gtgaatccac aattgaaaat aaaaagggcg ccactattaa gggcgccctt
      901 ttctttctga cagtaccgat ttaccggtgc aggttttacc agtgtcccct ttaccaatgc
      961 cccctttacc aatgtactcc gcgcatgatg taggcgaacg ccatctgctc cggcgacacc
     1021 actgacggct gggagtcgtc cttcttgccc tgggccgccg ccgagtccgg gaacatgcgg
     1081 aaaccggtgt tgatgatgat ctcggcaaac ttcggcgcca ctgcgtgcac aacctgaccg
     1141 aacacaccca gcttggtggc aatgcgcttg ctgcggtaaa tcagcgcctc gccgaccatg
     1201 tccgccgcct cttccgggct gatggtgggc acgttgtcgt aaatcttggt gggcgcgatc
     1261 atcggcgtgc gcaccagcgg catgttgatg gtggtgaagt gcacgttgca gtcggaatac
     1321 tccgatgccg cgcagcgcga gaatgcatcc agcgccgact tcgatgccac gtaggcactg
     1381 aaccgcgccg gataggtcag cgcaccgatc gatgaaatat tgatgacgtg cccgctgcga
     1441 ttcttttcca ttaccggcag gaaattcatc atcagccgca gcgcgccgaa gtaattcagc
     1501 tgcatggtgc gctcgaaatc gtgaaaacgg tcgtaagaca gtgagatcga acggcggatg
     1561 gaccggcccg cgttattgat cagcacatcg acgcggccga aatccgccag cacctgctgg
     1621 gcgaaacggt cgcagtcttc catgttggcg atgtccacgc tgtaggtgaa tgcctcgcct
     1681 cccgccgccg taatttcttc cttggtctct tccagctttt ccggcgtgcg cgccgccaga
     1741 atcacttttg cgcctgccgc tgccagtttc agcgccgacg ccttgccgat gccggacgaa
     1801 gcaccggtca ccaccaccac cttgcctttg acccgccctt ccagcgtgcg gtcgatgaac
     1861 agttcgggat ccaggttgcg ttcccagtaa tcccacaggc gccaggcgta ggtatccagg
     1921 cgcgggcagc tgatgccgga accatccagc gcggcgcggg catcgcgatt gtcgaacttg
     1981 gtgtggtact gggtgaattt cagcagcgaa cggggaatac ccagatcttc cagcacggtg
     2041 ttggtgatgc gtttcaccgg cggcagcgtg cccagggtct ggcgcaggaa cggcggcata
     2101 aagctgaaca tgcgggtgtc gatacgcatg gtcatctgcg gcgcatggcc ggcgcgggca
     2161 aaaatgttga tcacttcgcc gatcttgtaa tgatccacat cggtgaggtg gaagcatttg
     2221 ccatccagtc cgtccttgtg cgccagataa tccatggcat ccactacgta atccactggc
     2281 actatgttga agcgcccgcc ttcgatgcca atcaccggca tccagggcgg cagcagcttg
     2341 cgcaatttct tgatcaggcc gaagaaataa taagggccgt cgatcttgtc gatctcgccg
     2401 gttttcgaat gccccaccac catggccggg cgataaatgc ggaacggaat cttgcagtcc
     2461 ttgcgcacaa tggcttccga atcatgcttg gtgcgcagat agggatcgtt gagtttttcc
     2521 gcttcctcga acatgtcctc gcggaaaatg ccggggtaca tgccggccgc cgcaatggaa
     2581 ctgacatgat ggaaacaacc ggcggccatt tccttcgccg ctttcaccgc atggcgggtg
     2641 ccgtcaatat tggcactgac ctgcaccgct tccggcgccg acagatcata aatcgccgcc
     2701 aggtggaaaa aatgttttac cttgcctttc agcatcgcca tgtcatcggc gctgatgccc
     2761 agcttgggct gggtaatgtc gccgattacc ggaacgatcc ggccttcggt atcgcctacc
     2821 agtgctttca gcgtatccag cttgccgagg gatgcctcgc gcatcagcac ataaatggtg
     2881 ccctgacgct tgagcagctt ttccagcaaa aagcgcccga taaagccact ggcgccggtg
     2941 acgaaataag tcatgttggt atcctcatct acccttgaaa aagaaaactg cgactcacaa
     3001 acacttacac gtgtgacaac tgccccgaga acacgggtac tcccaatact gcgttcttga
     3061 tcgaggcaaa ttcatccacg aaatcgtcga tcatggcctg tgggtccgga atcagcgcgg
     3121 cgtccgcgac aaagccgaat tccaccttgt tgtcgtaact cagaatgctc acaccgatgc
     3181 cgatgccacc ggactgcggc acccagaaca tgggcttggc caatttccgc cccttcaggt
     3241 acaccggctc ggcgggcccc ggcacgttgg tcatcaccgc cgacgccttg ctggaaaaga
     3301 aatcgaggaa gaatttttcc accgcacgcg ggaagaagcc gagaataccc agcacgttgt
     3361 aactcatctg cgcctgcacc ccgctcttga gctgacgcat ggcctgacgc acagcctgga
     3421 tacggcgctc gaccgtgggc tcacccaccg gcaggttcag gtacaccagg ccaaagcggt
     3481 tgcccaactg cagggcgtct tcgatggggc gcaggttgac gggcaccgtg gcccgcagct
     3541 gcacgccgtt gaccggctcg ccgatccgct ccagatagcg gcgcagactg ccggccacac
     3601 agcccaggat cacatcattg atggtgcacc ccatggcttt gctgacccgt ttcacttcat
     3661 tgatgtccag cgaaggcgcc cagcctacct gcttatgggc agtcagctta cgtttgtacg
     3721 ctgttttgga atccggccgg gccagaatca cccgcatggc ttcaaacaac caggccacgg
     3781 agaacccggt ataaaaggcc gctgacgtga gcgcgaactc tgcccggtgc agcagcgcat
     3841 cagagcgggg cttgcgctgg ggaaacttga tgttggcggc cggagaactg tgcaagacag
     3901 acttgtcggc aatggaatcc agcactgaaa tcagcgcaat tccatcggca tagctgtggt
     3961 gcaccttcac catcaccgcc gtgccttcct tgtatttcgg cacgaaaaag aagcgccaca
     4021 acggcttgtc cgcatccagc ggcgccggaa tctgccggct cacggccgcc cggatttccg
     4081 cctcattgca gcattcctgc agcccttcca gagcggacag gtgatattgc atgtccacct
     4141 ggggcagttc ttcccacaga tactggttac cgacctgcac tggccgctgc cggaagcgct
     4201 tgtgccgccg gatcagccgg tcttccagga tctctttgaa atcctcgtaa ctcattggct
     4261 cgtcaaacat caacaagcca ttgattacca tcaggttatc gggtgaatcc atgcgcagcc
     4321 acgcagtgtc gactacgctc atgggctcca gcgcccggtg attggaagtc ctcataccat
     4381 tgttccctta gccccgagac agcgttgcag ttgccattgt tgggtagatg aattcgcttt
     4441 tcagcgtaca agtgtttgcc ataataacga acgtatgccg aaatgcaaca ccctcttaag
     4501 ggcgaatgtc gcaataagcc agacatttgg cgcgaacggc tttacaaaac aaaaagatgc
     4561 aaaaacgaaa ttgaaaaacc agctaacaaa tgtacacttc cattgcttga ccaattagcc
     4621 agtactgcgc aatccgcgcg gtgggcagtc ccattccagg actggacacg caaccccaag
     4681 gatagtgcag ccaacccgct ttcgcctttg agcccatgac cgaaccactg acccgagccg
     4741 aacagaaaca acagacccgc gaaaccatca tgacggcggc cgtccgcaag atgaatgacg
     4801 accgtggctt ctccagcctg agcctgcgcg aagtggcgaa agaggccggc atcgcgcccg
     4861 cctcgttcta tcgtcatttt gaaaacatgg aggatctggc gctggcgctg gtgaaggagg
     4921 ccggcgatgc gctgctcaag atcatgagcg tgacacggga atcggaagag aaaggcgaaa
     4981 acattctgga aaacgcagtc gcaatgtgca tggcgcactt tcgcgaaaac ggcccgctgt
     5041 ttcgcgtact ggcgcgcgag tccacgggca attcacccat tatccgccgc gccattcagc
     5101 gggtgctgaa cgaactgaac aagcagttgg cggccgtgat cgagctggaa atgaaaaaac
     5161 gcggccgcgc catcaccgac gcggcctgga tagcagaagc catcatcacc atcattttct
     5221 acattggcgt ttccaacatc gacctttcct acgcccgcca gaaggaagca gagaaacgcc
     5281 tggtgcatca cgttcgcgcc atttactggg gcgccgaagc catggccagc tcgcagcatc
     5341 tggaacggca attgatgctg tcctgattgt tcacatcagg ccttgatagc aatgcccgca
     5401 atgaccggcg ccaccagcgc cggcaccttc ggatgaatga aatcatcaat tgactcatag
     5461 cgaaagcctg cggcctcgaa cagtgcgcgg gtgtcacggt tgatctcgca accacaggcc
     5521 atcttccgcc acaacggatt cagtcggttc tgccacgcac gcgtggtatc ccggggcgac
     5581 gccacgtgct cgaaaaacac cagccggccg ccgggcttca atacacgaaa cgcttcccgc
     5641 gccgcatttt ccggctcggg aatcgtgcag aacaccaggc aggccaccac cgtatcgaaa
     5701 tccgcatcgg caaacggcaa cgc
//
LOCUS       DASUEG010000997         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_634997, whole genome shotgun sequence.
ACCESSION   DASUEG010000997 DASUEG010000000
VERSION     DASUEG010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634997"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFX11_18705"
     CDS             <1..1117
                     /locus_tag="VFX11_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4940712.1"
                     /translation="FLDLLSALKNRIIYADMDQFYHLSRTVLVKDEKHFDKFDRAFKK
                     YFDGLEAIDDLFTSLIPDEWLRKEIEKTLSKEEFEKLKQLGDFEKLMEEFKKRLEEQK
                     KRHQGGNKMIGTGGTSPYGAYGQNPAGFRMAGNSRNKNAVKVWEKRQYRNLDDSVELG
                     VRNIKMALRRLRKFTREGMPDQLDIDDTISSTARNAGYLDIKLVPEKRNGVKVLIFFD
                     VGGSMDPYVRTCEELFSAARAEFKHMEYFYFHNFIYEGVWKDNMRRWTDRTNVWDVIH
                     TYSSDYKVIFVGDATMSPYEISSVGGSVEHWNEEAGEVWMRRITQHFDKVAWLNPEPE
                     SSWEHTTSVVWTRKLLENRMFPLTLKGLEDGMRYLSK"
     gene            complement(1218..1691)
                     /locus_tag="VFX11_18710"
     CDS             complement(1218..1691)
                     /locus_tag="VFX11_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX4940713.1"
                     /translation="MDYLENIPFDELQEGQTAEYVKTVSEDDIKLFAIISGDTNPLHL
                     DPDYAKTTRFGQCIAHGSICAIIVSAAVATRIPGPGTIYAGQEMKFKKPVFPGDTLTA
                     TLTVQEKKRKGNIVLIDCVIRNQHGDTVFTGVSTVVAPTEKVRIAAVKLPTVELR"
     gene            complement(1794..2777)
                     /locus_tag="VFX11_18715"
     CDS             complement(1794..2777)
                     /locus_tag="VFX11_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4940714.1"
                     /translation="MQSEKLYLTSLDNHKIFVRKWSPDAAATSPRAIIHILHGMAEHC
                     TRYEPIAERMVDAGYVVFAHDHRGHGRSIPQGGLVGHYADHNGWPLVIADTRKVNEYI
                     HQQYPGIPVIIVGHSMGSFITQGYLIQHGRTVDAVVLSGSAFNTPMAIRSARMLAHLE
                     KLRTGPKGRSSLIDMATFASYNRQVAKSPRTGSDWLSRDPCEVDKYVGDPLCGFLCTN
                     QLWLDLTGGLEQISRLRNIRNIPPALPILLISGERDPLSFDNSRHGIEKLAEHLRSGG
                     VREVSVRLYEDARHEIFNEINRHEVIDDVLAWLDTRFSTRENVRRTDAAMA"
     gene            3138..>4216
                     /locus_tag="VFX11_18720"
     CDS             3138..>4216
                     /locus_tag="VFX11_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940715.1"
                     /translation="MVDRIWTQHYPEGVSPEISYDRFKTVLDVFENACRRYADRPAFS
                     NMGCTITYGQLDKLSADFAAYLQNHTSLKKGDRIALQMPNLIQYPIALFGALRAGLVI
                     VNTNPLYTAREMEHQFNDAGCKALVALANFGDLIEEVVPRTGIKHVIITQVADMQPPV
                     KRLLMNLIIKHVKKMVPSFNIPGAVSFTKAMSKGASSTFDQDHRPEPDDIAVLQYTGG
                     TTGVAKGAMLTHRNLASNMMQFDFILRALRPGQEIAITPLPLYHIFSFTVNCLGLMSL
                     GANIVLITNPRDIPGFVKELAKWRFTTFTGLNTLFVALMNNPDFQKLDFSSLKLTIAG
                     GMALQKDTADRWEAMTGNKIAEGFG"
BASE COUNT          903 a         1199 c         1243 g          871 t
ORIGIN      
        1 atttctcgac ctgctgtctg cgctgaagaa ccggatcatc tacgcggaca tggaccagtt
       61 ttaccatctg tcccgtaccg tgctggtgaa ggatgaaaag cacttcgaca agttcgaccg
      121 cgccttcaag aaatattttg acggcctgga agcgatcgac gatctgttca cctccctgat
      181 cccggacgag tggctgcgca aggaaatcga gaagaccctc agcaaggaag aattcgaaaa
      241 gctgaaacag ctgggcgatt ttgaaaaact gatggaggaa ttcaagaagc ggctggagga
      301 acagaagaag cgccaccagg gcggcaacaa gatgatcggt accggtggca cttcgccgta
      361 cggtgcctac ggccagaacc cggccggctt ccgcatggcg ggcaactctc gcaacaagaa
      421 tgcggttaaa gtctgggaaa agcgccagta ccgcaacctg gacgattctg tggagctggg
      481 ggtgcgcaac atcaagatgg ccctgcgccg cctgcgcaag ttcacccgcg aaggcatgcc
      541 ggaccagctc gacatcgacg acaccatttc ctccaccgcg cgcaatgcgg gctacctgga
      601 tatcaagctg gtgccggaaa agcgcaacgg cgtgaaggtg ctgatcttct tcgacgtggg
      661 cggttccatg gacccttacg tgcgtacctg cgaggaactg ttctccgccg cacgcgccga
      721 gttcaagcac atggagtact tctatttcca taacttcatt tatgaaggcg tgtggaagga
      781 caacatgcgg cgctggaccg atcgcaccaa tgtgtgggac gtgattcaca cctacagctc
      841 agactacaag gtgattttcg tgggtgacgc caccatgtct ccctacgaga tcagctccgt
      901 gggcggcagt gtcgagcact ggaacgagga agccggggaa gtctggatgc gccgcatcac
      961 ccagcatttt gacaaggtgg cgtggctgaa tccggaaccg gaatccagct gggaacacac
     1021 tacctcggtg gtctggacgc gcaaactcct ggaaaaccgc atgttcccgc tgaccctgaa
     1081 agggctggaa gacggcatgc gctatctgtc gaaataaaga cgctcaaatc aagggctgtt
     1141 caaacagcag aaaaaacaaa ccccggttcg tccggggttt ttattgtcag cctgatgctg
     1201 agggcgttgc tggttgctca gcgcagttcc accgtcggca gtttcaccgc agcaatgcgg
     1261 actttctcgg tcggtgccac caccgtcgac acacccgtga acaccgtatc gccgtgctgg
     1321 ttgcggatca cgcagtcgat cagcacgatg ttgcccttgc gtttcttctc ctgcaccgtc
     1381 agtgtcgcgg tcaatgtgtc gccggggaaa accggcttct tgaatttcat ttcctgaccc
     1441 gcgtagatgg tgccgggccc gggaatgcgg gtggcgaccg ccgcgctgac gataatggcg
     1501 cagatcgagc catgggcgat gcactggccg aagcgggtgg tcttggcgta gtcgggatca
     1561 aggtgcagcg gattggtgtc accggaaatg atggcaaaca gtttgatatc atcctcggaa
     1621 acggttttca cgtattccgc cgtctggcct tcctgcagtt cgtcgaacgg aatgttttcc
     1681 agataatcca tcgttacatc ctcctcaaac ggatagccct gcattctaat cagcgtgcag
     1741 ggctaccggt atcgaaagag tgtaagcgaa tttagatttt ggcacgtgcg gcgtcaggcc
     1801 atcgcggcgt ccgtgcggcg cacgttttcc cgggtggaaa aacgggtgtc cagccaggcc
     1861 agcacgtcgt cgatcacttc gtggcggttg atctcgttga aaatctcgtg gcgcgcgtct
     1921 tcgtacagtc ttaccgacac ctcgcgcaca ccgccgctgc gaagatgctc cgccagcttt
     1981 tcgataccgt gacggctgtt gtcgaagctg agcggatcgc gttcgcccga gatcagcagg
     2041 atcggcaggg cgggcggaat gttgcggatg ttgcgcaggc gcgagatctg ctccagtccg
     2101 ccggtcaggt ccagccagag ctggttggtg cagagaaaac cacacagcgg atcaccgacg
     2161 tacttgtcca cttcgcacgg atcgcggctc agccagtcag agccggtgcg cggacttttg
     2221 gcgacctgac ggttgtagct ggcaaaggtg gccatgtcga tcaggctgct gcgacctttc
     2281 gggccggtac gcagcttttc cagatgagcc agcatgcgcg ccgagcgaat cgccatcggc
     2341 gtattgaagg cagagcccga cagcaccacc gcatccactg ttctgccatg ctggatcaga
     2401 tagccctggg tgataaagga gcccatgctg tggccaacga tgatcaccgg gatgccaggg
     2461 tattgctggt gaatatactc attcaccttg cgggtatcgg cgatcacgag gggccagcca
     2521 ttatggtcgg catagtgacc taccagcccg ccctggggaa tggaacggcc gtggccccgg
     2581 tggtcatggg caaacacgac atagccggca tccaccatcc gttcggcgat cggttcgtag
     2641 cgggtgcagt gttcggccat gccgtgcagg atatggatga tggcgcgggg cgatgtcgcc
     2701 gcagcgtccg gactccactt gcgtacaaag atcttgtggt tgtccaggct ggtgagatag
     2761 agcttttcag attgcatgat ggcgaccccg tgacgttccc tggcgatagg cgttggcatc
     2821 agatgccatg attttttgcg tggcggtaat tgtatatgct ggcgtccgat ccggccaatt
     2881 tacctccccg gctattgtgg gttgcatcga agcgcagaat aaatagtaca gaatttaacg
     2941 acttatcggt cacgcgtggc ggcggggagc gggcactggt ttgccggaat caaaactggt
     3001 aacctcacgc cgccacacgc cggacagacc attttcacgt catttgcatt ggtctggatc
     3061 aaggggatgg gcgtcaattc agcaaattgc ccatgggcaa acaggctagg gaatccacgc
     3121 agcgctgggg tatcagcatg gttgatcgca tttggacaca gcattatccg gaaggggttt
     3181 cccctgaaat ttcctacgac aggttcaaga cagtgctcga tgtctttgaa aatgcctgtc
     3241 gccgttatgc ggaccgcccg gccttcagca atatgggctg caccatcacc tatggccagc
     3301 tggacaagct gagcgccgat ttcgccgcct atctgcagaa ccacaccagc ctgaaaaagg
     3361 gcgaccgcat cgccctgcag atgcccaacc tgatccagta tcccattgcg ctgttcggtg
     3421 ccctgcgcgc cggtctggtg atcgtcaaca ccaatcccct gtacaccgcc cgtgaaatgg
     3481 agcatcagtt caatgatgcc ggctgcaagg cgctggtggc cctggccaat ttcggcgatc
     3541 tgatcgagga agtggtgccg cgtaccggca tcaagcacgt gatcatcacg caggtggccg
     3601 acatgcagcc gccggtgaag cgcctgctga tgaacctgat catcaagcat gtgaagaaaa
     3661 tggttccctc tttcaatatc ccgggtgccg tgtcgttcac caaggccatg agcaagggcg
     3721 catccagcac cttcgaccag gatcaccgcc ccgagcccga cgatatcgcc gtgctgcagt
     3781 acaccggtgg cacgacaggt gtggccaagg gcgcgatgct gacccacagg aacctggcgt
     3841 cgaacatgat gcagttcgat tttatcctgc gggcactgcg tcccgggcag gaaattgcca
     3901 tcacgccgct gccgctctat cacatctttt ccttcacggt gaactgcctg ggtctgatgt
     3961 cgctgggcgc caatattgtg ctgatcacca atccgcgcga cattcccggt tttgtgaagg
     4021 agctggcgaa gtggcgcttc accaccttca ccggcctgaa caccctgttc gtggcactga
     4081 tgaacaatcc cgacttccag aagctggatt tcagttcgct gaagctcacc atcgccggcg
     4141 gcatggcgct gcagaaagac accgccgacc gctgggaagc catgaccggc aacaagatcg
     4201 cggaaggctt cggcat
//
LOCUS       DASUEG010000998         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_655507, whole genome shotgun sequence.
ACCESSION   DASUEG010000998 DASUEG010000000
VERSION     DASUEG010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655507"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFX11_18725"
     CDS             complement(<1..469)
                     /locus_tag="VFX11_18725"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX4940716.1"
                     /translation="MHQRLVPATLIPGILDLAIKNRINVEKIFATAKVDLAVIGHSGT
                     FLTLEQILRLFNAAYAAMNDPAFGIRLGESVQYHSLDLVGQLVATSRNLEEALDELFQ
                     FKDLVAPFTLFSLDLKGKNAILGFSIDTSLIKKNLPVHQDLVSTTVVTIANAIA"
     gene            complement(605..3127)
                     /locus_tag="VFX11_18730"
     CDS             complement(605..3127)
                     /locus_tag="VFX11_18730"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4940717.1"
                     /translation="MKQGHLVGSCRAHLFIHLLCWLLLLVLPVVTQAADPTSTHSASQ
                     AGRQSPIIAILPSHLDGYDLGPFMSHWYDDTEHSSLEAARGAQRDGRFAPMERLSPNM
                     GFARGHHWFHVVMQNALGFRRMILLEVDYPILDQIEFFCFGPGQEPTYFPAGDHVQFS
                     SRVVKVRNYVVPLNLEPYRSTECLIRARSTSNVIFPLRAFDNIPYIEKSHRVEWGLGI
                     LYGLAFALLFYNLMIYISTREAVYLYFILHVIGGLGYTTAMDGTLAPFWIGLELQDLG
                     LLISICLSAGAGILFGLEFLDIRRTWPAANMVGTVMFLGMMGFSVLVLVAPLMLSHIV
                     ITLFALVIGIYLFVLGVKRWRDGYGPAQVYVLGYGTVMVMIVWMALNVLFLRADVRWI
                     TYGMSLAWLCELLVLSVALGYRIQDNRRERTALSQQVQAVLHESNSKTEFMAKVSHEI
                     RTPMNGIMGLVELLLGTALGPDQRRYLNAIRHAGHGLQEVINDVLDFSRIEAGKMNLV
                     RHPFELQQMLQDVCAIYEFEARRKRIELGCFIAEGTPLQLIGDASRIRQVLLNVLSNA
                     LKYTEQGFVHINVQLTDAILSDQLMVRFEVEDSGIGISPRDQRKLFQSFSQIQHEHRQ
                     DTPGSGLGLLISQQIVELMGGEMGVKSELGRGSCFWFNLPLTLPDNVQVAEAAIALDL
                     FDGVTSAAMDSEHTPAPVVPIRATSGPCVLVVEDNEINQNVMTGFLQKLGIKPDLADN
                     GRTAVEMVQRGHTYDLILMDCEMPVMDGYEAASRILKWQRANGLTPTPIVALSAHALD
                     KHRDMAFEAGMVDYLSKPIGFRQLVDKIGRYIDLPADAAMLF"
     gene            complement(3197..>3472)
                     /locus_tag="VFX11_18735"
     CDS             complement(3197..>3472)
                     /locus_tag="VFX11_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940718.1"
                     /translation="GFSSLYQIKLLPIDMVKIDGSFIRHLPQEPEDQALVRAIVEVAR
                     VFGLMTVAEFVENEAIVDMLAELGVDYAQGYHIAEPRFFDRIWGETP"
BASE COUNT          794 a         1070 c          950 g          658 t
ORIGIN      
        1 cggcgatggc attggcgatg gtcaccaccg tggtcgacac cagatcctgg tgcaccggca
       61 ggtttttctt gatcagggaa gtgtcgatgg aaaaaccgag gatggcattc ttgcccttga
      121 gatccagcga gaacagcgtg aacggcgcca ccagatcctt gaactggaac agctcgtcca
      181 gcgcttcttc cagattacgg ctggtggcca ccagttgtcc caccagatcc aggctgtggt
      241 actgcaccga ttcccccagg cgaatgccaa aggcaggatc gttcatggcc gcataggcgg
      301 cattgaacag acgcaggatt tgttcgaggg tgaggaaggt accggaatgg ccgataacag
      361 caagatccac cttcgccgtg gcgaaaatct tttcgacatt gatgcgattt ttaatcgcca
      421 gatcaagaat gcctggaatc agcgttgccg gaaccaaccg ttgatgcata aagacactct
      481 gcagtacccg aaataacagc caattagcac aagtaaatca gtcagctgaa aatgtaatat
      541 ctggccaagc atactgactt tgaattgact aaacaacttt tgtccgcatc ctttcggtgc
      601 gcactcagaa aagcatggca gcatccgccg gcaaatcaat atatcggccg attttgtcta
      661 ccagctggcg gaaaccgatc ggcttcgaca ggtaatccac cattccggcc tcgaaagcca
      721 tgtcgcggtg cttgtccagt gcgtgagccg acagcgccac gatcggcgtc ggggtcaggc
      781 cgttcgcccg ctgccacttc aggatacggg aggccgcctc gtagccatcc atcactggca
      841 tctcacagtc catcaggatc agatcgtaag tgtggccccg ctgtaccatt tccacggcgg
      901 tgcggccatt gtccgccaga tcgggcttga tacccagttt ctgcaggaag ccggtcatca
      961 cgttctgatt gatttcattg tcctccacca ccagcacaca agggccactg gtggcgcgga
     1021 tcggcaccac cggtgcgggt gtatgctcgg aatccatggc ggcggatgtc acgccgtcga
     1081 acagatccag cgcgatggcc gcctccgcca cctgcacgtt gtccggcaag gtcagcggca
     1141 ggttgaacca gaaacaggag ccgcgcccca gctcgctctt cactcccatc tcgcccccca
     1201 tcagttccac aatctgctgg ctgatcagca atcccaggcc gctgcccggc gtgtcctgcc
     1261 ggtgctcatg ctggatctgg gaaaagctct ggaacagctt gcgctgatcg cggggggaaa
     1321 tgccgatgcc ggaatcctcc acctcgaacc gcaccatcaa ctggtcggac aggatcgcat
     1381 cggtcagctg cacgttgatg tgcacgaagc cctgctctgt gtatttgagc gcattcgaca
     1441 gaacattcag cagcacctgg cggatacggg aggcatcgcc gatcaactgc aacggcgtgc
     1501 cctcggcgat gaagcagccc agttcgatgc gcttgcgccg cgcctcgaat tcataaatcg
     1561 cgcagacgtc ctgcaacatc tgctgcagtt cgaacgggtg ccgcaccaga ttcatcttgc
     1621 cggcctcgat gcgggaaaaa tccagcacat cgttgatcac ttcctgcaac ccgtggccgg
     1681 cgtgacggat cgcgttcagg tagcggcgct gatccggtcc cagcgcggta cccagcagca
     1741 actccaccag ccccatgatg ccgttcatgg gcgtgcgaat ctcgtggctc accttggcca
     1801 tgaattcggt cttgctgttg ctttcatgca gcaccgcctg cacctgctgg ctcagcgcgg
     1861 tacgctcgcg acggttgtcc tgaatgcgat aacccaacgc caccgacagc accagcagtt
     1921 cgcacagcca ggccaggctc atgccgtagg tgatccagcg cacatcggcg cgcaggaaca
     1981 gcacattcaa cgccatccat acgatcatca ccatcaccgt gccatagccc agcacataca
     2041 cctgcgccgg accatagcca tcacgccagc gcttgacacc caatacaaac agataaatgc
     2101 cgatcaccag cgcgaacagg gtgatgacaa tgtgcgacag catcagcggc gccaccagca
     2161 ccagcacgct gaagcccatc atgcccagaa acatcacggt gcccaccatg ttcgccgccg
     2221 gccaggtacg gcggatgtcc agaaattcca gtccgaacag gatgccggca cccgcgctca
     2281 gacagatcga gatcaacagc ccgagatcct gcaattccag cccgatccag aaaggtgcca
     2341 gagttccgtc catcgctgtg gtataaccca ggccgccgat cacgtgcaga atgaaataca
     2401 ggtacaccgc ttcgcgggtg gagatgtaga tcatcagatt gtagaacagc agcgcgaagg
     2461 ccagtccata cagaatgccc agcccccatt ccacccgatg gctcttttca atatagggaa
     2521 tattgtcgaa ggcacgcagc ggaaaaataa cgttcgacgt ggagcgcgcg cggatcaggc
     2581 actcggtgga tcggtagggt tccagattca gcggcaccac atagttgcgc accttcacca
     2641 cgcggctgga gaactgcacg tgatcgccag cgggaaaata ggtgggttcc tgtccaggcc
     2701 cgaagcagaa aaattcgatc tggtcgagga tggggtagtc cacttcaagc aggatcatgc
     2761 ggcgaaaacc cagcgcgttc tgcatcacca catggaacca gtgatgtccg cgggcaaatc
     2821 ccatattggg cgacaggcgt tccatcggcg caaatcggcc gtcacgctgc gcgccgcggg
     2881 cagcctccag tgatgaatgc tcggtatcgt cataccagtg cgacataaac ggccccagat
     2941 cgtagccgtc gaggtggctg ggcagtatcg caatgatcgg ggactgccgg ccggcctggg
     3001 acgcggaatg agtactggtt gggtcagccg cctgcgtgac aaccggtaac accagcagca
     3061 acagccagca cagcagatga atgaacagat gggctcggca actgccgacg agatgtccct
     3121 gcttcacaat gggattccat tatccttgtt ctctggtccg ctagaatata ccgcgccgtc
     3181 actggctcaa agcgggttat ggtgtttcgc cccagatccg gtcgaaaaaa cgcggttccg
     3241 caatatggta gccctgggca tagtccacgc ccagctcggc cagcatgtcc acaatggcct
     3301 cgttttcgac aaattctgcc actgtcatca acccgaatac ccgcgccact tccacgatcg
     3361 cgcgcaccag tgcctggtcc tccggctcct gcggcagatg gcggatgaag gaaccgtcga
     3421 tcttcaccat gtcgatgggc agcagcttga tctggtacag cgacgaaaag cc
//
LOCUS       DASUEG010000999         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1166488, whole genome shotgun sequence.
ACCESSION   DASUEG010000999 DASUEG010000000
VERSION     DASUEG010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166488"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFX11_18740"
     CDS             complement(<1..983)
                     /locus_tag="VFX11_18740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX4940719.1"
                     /translation="MDRTTESKKLTAAPGARRYWPVHLLQRVFRDYPGSMAVRLWDGN
                     TLEFGTDAPRFTLNFRHPRVFRDLVLFRDPLRLAEAYIRGKVDLDGDLYSALALRNYL
                     QAVCLTVAERAAFLARAALMSGVPRSNRDFREPAGSRAIRARLSPRHSRAVNRQAIAF
                     HYDVSNDFYRLWLDEQMVYSCAYFEAADDSLDQAQRNKLGHICRKLRLKPGERLLDIG
                     CGWGALIRWAATHYGVSAHGITLSRGQYEHCQQRIAAEGLRDRVSVELVDYRDLPGEA
                     VFDKIVSVGMFEHVGVKNYPIYFGAVRRLLKPGGLFLNHGITQETEGWDRK"
     gene            complement(1022..1249)
                     /locus_tag="VFX11_18745"
     CDS             complement(1022..1249)
                     /locus_tag="VFX11_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX4940720.1"
                     /translation="MLHDGWALWGMHLFWWLFWITMIVALFSLFTPVPRGRRRDTPLE
                     LLQRRYAAGEITSAEYEERKTKLERDATPVR"
     gene            complement(1342..2004)
                     /locus_tag="VFX11_18750"
     CDS             complement(1342..2004)
                     /locus_tag="VFX11_18750"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX4940721.1"
                     /translation="MDSTSPPAYGLWSLVIINSFIFIIFAFSFAKPQSTRDWRSFGAF
                     SGFLVALFTEMYGFPLTIYLLSGWLTTQFPGIDWLSHDAGHLLETLFGWSGNPHFGPF
                     HLLSSAFLVGGFWVLASAWKVLFEAQRTDRLATTGAYRYVRHPQYGGFVLIMFGFLLQ
                     WPTLVTLIMFPILVFMYLRLAKTEETWMQARFGDEYRHYAAQTPAFFPRWRGAKAIGR
                     IS"
     gene            complement(2004..2306)
                     /locus_tag="VFX11_18755"
     CDS             complement(2004..2306)
                     /locus_tag="VFX11_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011992998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HEX4940722.1"
                     /translation="MAHDHGANDNRQHNRRSNWVFIGFLAIGGYFLITEHRAHLLPFL
                     GYLPFLLLLACPLMHIFHHGGHGGHESHGSGQKESTSDQQKDKPTDHQSHTTKGES"
BASE COUNT          509 a          724 c          672 g          449 t
ORIGIN      
        1 ctctttctgt cccaaccttc ggtctcctgg gtgatgccgt ggttcaggaa cagcccgccc
       61 ggtttcagca ggcgccggac cgcgccgaaa tagatcgggt aattcttcac cccgacgtgc
      121 tcgaacatgc cgacgctgac gatcttgtcg aacacggctt cgcccggcag atcgcggtaa
      181 tcgacgagtt ccacactgac ccggtcccgc aatccttcgg cggcgatccg ttgctggcag
      241 tgttcgtact gaccacggct aagggtgatg ccgtgggccg acacgccgta atgcgtggcc
      301 gcccagcgga tcaaagcgcc ccagccgcag ccgatgtcca gtagccgttc gccgggcttg
      361 agccgtagtt ttcggcaaat atgcccgagc ttgttacgtt gcgcctggtc caggctgtcg
      421 tccgcagcct cgaaataagc gcaggaataa accatctgct cgtccagcca cagccgataa
      481 aagtcattgg atacgtcgta gtgaaaggcg atggcttgcc ggttcacggc gcgcgagtgg
      541 cgcggcgaaa gccgtgcgcg tatcgccctt gatcccgccg gttctcggaa gtcccgattg
      601 ctgcggggca caccgctcat gagcgctgcc ctggcgagaa acgcggcccg ttcggcgacc
      661 gtcaaacaca ccgcctgcaa gtaattcctc agcgccagcg ccgagtaaag atcgccatcc
      721 agatcaacct tgccgcgtat ataggcctcg gccaagcgca gcggatcgcg aaacagaacc
      781 agatcacgaa aaacccgcgg atgccgaaag ttcaacgtga atctcggcgc gtcggtgccg
      841 aattccagcg tgttgccatc ccacaggcgc accgccatgc tgcccgggta atcccggaac
      901 acgcgctgca gcaaatgcac gggccagtac cgccgcgcgc cgggcgcggc cgtcagtttc
      961 ttgctctcag tagtcctgtc catctgctac ccctccctcg ctttgtcgct ggtccacact
     1021 gtcaccggac cggcgtggcg tcgcgttcga gcttggtctt gcgctcctca tattcggctg
     1081 acgtgatctc gcccgcggca taacgacgct gcagcagttc gagcggcgta tcgcgccgcc
     1141 ggccgcgcgg caccggcgtg aacagcgaaa acagggccac gatcatcgtg atccagaaca
     1201 gccaccaaaa aaggtgcatt ccccacaaag cccagccgtc atgcaacatg tctcacctcc
     1261 gtattggcca gaaaattccg agaacgggcg cggacctcga acttgcaggg tatctttcat
     1321 ggatgaccct ggattactgg attagctgat tcgcccgatc gctttggccc cccgccagcg
     1381 gggaaagaac gccggtgtct gggcggcgta atgccggtac tcgtcgccga atctggcctg
     1441 catccaagtt tcctcggtct ttgccaaccg caggtacatg aacaccagaa ttggaaacat
     1501 gatcagggtc accagcgttg gccactgcaa caggaatccg aacatgatca gcacgaagcc
     1561 gccgtattgc ggatggcgta cataacgata ggcacccgtg gtggccagcc gatcagtgcg
     1621 ctgggcttcg aacagcactt tccatgctga tgccaaaacc cagaacccgc cgaccagaaa
     1681 ggcactgctc aaaaggtgaa aggggccgaa atgcggattg ccgctccaac cgaacaaggt
     1741 ctccagcaaa tgaccggcat cgtgcgacag ccagtcgatg ccggggaact gggtggtcaa
     1801 ccatccggac agcaggtaaa tcgtcagcgg aaagccatac atttccgtga acaacgccac
     1861 caggaacccg gaaaacgccc cgaacgaccg ccagtcccgc gtgctctgcg gcttggcgaa
     1921 gctgaaggcg aagatgatga agatgaacga gttgatgatt accagtgacc ataatccata
     1981 ggcaggcggg gatgtggaat ccattacgat tctcctttgg tcgtgtgact ttgatggtcg
     2041 gttggtttgt ctttttgctg atcactggta gattcctttt gaccgctgcc atggctctcg
     2101 tgcccaccgt gcccaccatg gtggaatatg tgcatcagcg gacaggccag cagcaggaga
     2161 aagggcaggt aaccgagaaa gggcagcagg tgcgcccgat gttcggtgat caaaaaataa
     2221 ccgccgattg ccaggaagcc gataaatacc cagttgcttc tgcggttatg ctggcggttg
     2281 tcgttggcgc cgtggtcatg tgccatgatg tgctccttca gaaaacagat agaaaaactg
     2341 ctcgcctctc cccc
//
LOCUS       DASUEG010001000         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1224078, whole genome shotgun sequence.
ACCESSION   DASUEG010001000 DASUEG010000000
VERSION     DASUEG010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224078"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..523)
                     /gene="nuoJ"
                     /locus_tag="VFX11_18760"
     CDS             complement(14..523)
                     /gene="nuoJ"
                     /locus_tag="VFX11_18760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX4940723.1"
                     /translation="MEIAFYSAGTIAVIATLKMLFSHDPVHGLLYLVISLLSVAVIYF
                     AVGAPFAGVLEMIVYAGAIMVLFVFVVMMLNLGETTVAMERAWLQPRAWVLPVILSSL
                     LLGLMVSFLANTQPHPIGTDVSIKQVGIALYGPYILAVELASLILLAALVTAFHLGRR
                     DEDAEGKSQ"
     gene            complement(538..1083)
                     /gene="nuoI"
                     /locus_tag="VFX11_18765"
     CDS             complement(538..1083)
                     /gene="nuoI"
                     /locus_tag="VFX11_18765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008014903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX4940724.1"
                     /translation="MKALIGIITGVLSQLRSMYMVFMHSFAKRDTLQYPEQPVYLPPR
                     YRGRIVLTRDPDGEERCVACNLCAAACPAQCISLQKAEREDGRWYPDFFRINFSRCIF
                     CGMCEEACPTTAIQLTPDFEMAEFRRQDLVYEKEDLLISGPGKNPDYNFYRVAGMAIA
                     GKPKGAAQHEADPIDVKSLLP"
     gene            complement(1094..2098)
                     /gene="nuoH"
                     /locus_tag="VFX11_18770"
     CDS             complement(1094..2098)
                     /gene="nuoH"
                     /locus_tag="VFX11_18770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003219563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX4940725.1"
                     /translation="MDWLTDETLAIIIAVLKSVVILLGVVVVAAFLIFVERRLLALWQ
                     DRYGPNRVGPFGLLQLIADMMKMFFKEDWTPPFADKLLFWIAPAIAVSGLLMAFCVVP
                     ITPTWGIADLDIGILFFLAMAGLSVYAVMFAGWASNSKYALLGAVRSTAQTLTYEVFM
                     GLSLMGVVAVAGTFNLREIVEAQKDMWFVIPQFIGFVTFFIAGVAVAHRAPFDQPEAE
                     QELAAGFFTEYSGLKWGMFFVGEYVGVVVVSALLTVLFFGGWYGPFLDVAPWLAFFYF
                     AAKVAFFIMLFILLRGALPRPRYDHMMAAGWKVLLPLTLINLLVTGAVVLMRTPPVAL
                     "
     gene            complement(2098..>4146)
                     /locus_tag="VFX11_18775"
     CDS             complement(2098..>4146)
                     /locus_tag="VFX11_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4940726.1"
                     /translation="PSVCQQCSVGCNTSPGERYGELRRIENRYNGTINHYFLCDRGRF
                     GYGYVNRSDRPRQPQQKSGQAWINLTQDQALDAALAALRGSKRVIGIGSPRASLESNF
                     ALQSLVGAGNFFSGVNSIENQLLKTTVKILREGPVKTPSLRDMEKYDAVLVLGEDVTQ
                     TAPRIALALRQAAKNKARQMAAERKVFEWQAEAIQNIGQRELSPVFIASLSATRLDDI
                     GADHFHGSPEDIARLGFAVAHAINADAPAVEGADAALLQRAQKIADALQGAKKPLIVS
                     GTSLLCEPVLQAAANVSRALHARNAETAISVVVPEVNSLGLGILDLPSLDEALNLLAI
                     READTVVVLENDLYRRAPASVVDNALAQAKNVIVLDHQLTDTARKGNLLLSATAFAET
                     DGTVVSQEGRAQRFFQVFDANFYHPEYAMQDSWRWLHGLATSMAGQEFAWGEMDDATA
                     ACAKAIPVLAGIVNAAPDSDFRIKGLKIARSPRRYSGRTAQRANLNVSELRAPQDADS
                     PLAFSMEGFFGPATPGPLTPFAWSPGWNSPQAWNKFQDEVGGHLREGDSGVRLIEPAT
                     QPAGFYQPCDPFVVKAGDWRIVPVHHIFGSDELAARAEVTESRQPEPYVGLAQADATA
                     LNVKEGAPLLLTFGTRHATLAVKILPGLASGMLALPVGINGVPVVSASDKVSIAGGAA
                     "
BASE COUNT          803 a         1277 c         1327 g          739 t
ORIGIN      
        1 agtggaatgc cgttcattgg gattttcctt ctgcgtcttc gtcacggcgg cccaggtgga
       61 acgcggtcac cagcgcggcc agcaggatca gcgaggccag ttccaccgcc agaatgtacg
      121 gaccatacag ggcgatacct acttgcttga tcgacacgtc ggtgccgatc ggatgcggct
      181 gggtgttggc caggaaggac accatcagac ccagcagcag gctggacagg atcaccggca
      241 atacccaggc gcgcggttgc agccaggctc tttccatggc aacggtggtt tcgcccaggt
      301 tcagcatcat taccacaaac acgaacagca ccatgatggc gccggcatac acaatcattt
      361 ccagcacgcc ggcaaacggc gcgcccacgg caaaataaat caccgccaca ctcagcagcg
      421 aaatgacgag gtagagcaga ccatgcacgg gatcgtggct gaacagcatt ttcagcgtgg
      481 cgatcactgc aatggtgccg gcagagtaaa acgcgatttc cacagtagtt cctcgtctta
      541 cggcagcagg gatttgacgt cgatagggtc ggcttcgtgc tgcgccgcac ccttgggttt
      601 gcccgcgatg gccatgcccg ctacccggta aaagttgtaa tcgggatttt tgccggggcc
      661 ggaaatcagc aggtcttcct tttcatatac cagatcctgc cgcctgaatt ccgccatctc
      721 gaaatccggg gtcagctgga tcgcggtggt ggggcaggct tcctcgcaca tgccgcagaa
      781 aatgcagcgt gaaaagttga tgcggaaaaa atccgggtac cagcggccgt cttcgcgctc
      841 ggctttttgc agcgagatgc actgggccgg gcaggcggcg gcgcacaggt tgcacgcgac
      901 gcagcgttct tcgccatcgg ggtcgcgggt cagcacgatg cggccacggt agcgcggcgg
      961 cagatagacc ggctgttcgg ggtactgcag ggtgtcacgc ttcgcaaagc tgtgcatgaa
     1021 caccatgtac atgctgcgca gttggctcaa gacgccggtg atgatgccaa tcagggcttt
     1081 cattgcagtt acctcaaagc gcgacgggtg gtgtgcgcat cagcaccacc gcaccggtga
     1141 caagaagatt gatcagggtg agcggcagca acaccttcca gccggcagcc atcatgtggt
     1201 cgtagcgcgg acgcggcagc gcgccccgca gcaggatgaa cagcatgatg aagaacgcaa
     1261 ccttggcggc aaaatagaag aacgccagcc agggcgccac atccaggaac gggccgtacc
     1321 agccaccgaa aaacagcaca gtcagcagcg cggataccac gacgacaccc acgtattcgc
     1381 cgacgaagaa catgccccac ttcaggccgg aatattccgt gaaaaagccg gccgccagtt
     1441 cctgttccgc ttccggttga tcgaagggag cgcggtgcgc aacggcgacg cccgcgatga
     1501 aaaaggtgac aaaaccgatg aattgcggaa tcacgaacca catgtctttc tgtgcttcga
     1561 cgatctcgcg caggttgaac gtaccggcca ctgccaccac acccatcagc gacaggccca
     1621 tgaacacttc ataggtgagc gtctgggcgg tggagcgcac ggctcccagc aacgcatatt
     1681 tgctgttgct ggcccagccg gcgaacatca cggcatacac cgacagaccg gccatcgcca
     1741 gaaagaacag tatgccgatg tcgaggtcgg caatgcccca ggtcggcgtg atcggcacca
     1801 cgcagaaggc catcagcaga ccactcaccg cgatggccgg cgcaatccag aacagcagct
     1861 tgtcggcgaa cggcggcgtc cagtcttcct tgaagaacat cttcatcatg tcggcgatca
     1921 gctgcagcag accgaacggg ccgacgcgat tggggccgta gcgatcctgc cacagcgcca
     1981 gcaggcgacg ctcgacaaag atcaggaacg ccgccaccac caccacgccc agcaggatga
     2041 cgacggattt cagcaccgca atgatgatcg ccagggtctc gtcggtgagc cagtccatca
     2101 tgcggcacct ccggcgatgc tcaccttgtc agaggctgag acgaccggca cgccgttgat
     2161 gcccaccggc agtgcgagca tgccgctggc cagccccggc aggattttca ccgccagggt
     2221 ggcgtgacgg gtgccgaagg tcagcagcag cggcgcgcct tcctttacgt tcagcgcggt
     2281 ggcgtcggcc tgggccaggc ccacgtaagg ctcgggctgg cgcgattcgg tcacttcggc
     2341 gcgggctgcc aattcgtcgc tgccgaaaat atggtgcacg ggcacgatgc gccagtcgcc
     2401 ggctttcacc acgaagggat cgcagggttg gtagaaaccg gcaggctggg tggccggttc
     2461 gatcaggcgc acgccggaat caccttcgcg caaatggccg cccacttcgt cctggaactt
     2521 gttccaggct tgcggcgagt tccagcccgg cgaccaggca aacggtgtca gcggacccgg
     2581 agtagcgggg ccgaaaaagc cttccatcga gaaggccagc gggctgtcgg cgtcctgcgg
     2641 tgcgcgcagt tcgctcacgt tcaggttggc gcgctgggcg gtgcggcccg agtagcggcg
     2701 tggcgagcgg gcaattttca gacccttgat gcggaagtcg ctgtccggtg cggcgttgac
     2761 gatgccggcc agtaccggga tcgccttggc gcacgcggca gtggcatcgt ccatttcgcc
     2821 ccacgcgaat tcctggccgg ccatggaggt ggccaggccg tgcagccagc gccagctgtc
     2881 ctgcatcgca tattccggat gatagaagtt ggcgtcgaac acctggaaga aacgttgggc
     2941 gcggccttcc tgactcacca cggtgccgtc ggtttcggcg aaggcggtgg cggacagcag
     3001 caggttaccc ttgcgcgcgg tgtcggtgag ctggtggtcc agcacgatca cgttcttggc
     3061 ctgggccagc gcgttgtcca ccacggatgc gggggcgcgg cggtacaggt cattttccag
     3121 caccacgacg gtgtcggctt cgcggatggc cagcaggttc agtgcctcgt ccagcgatgg
     3181 caggtccagg atgcccaggc ccaggctgtt cacttccggc accaccacgc tgatggcggt
     3241 ttcggcgttg cgggcgtgca gcgcgcggct gacgttggcg gcggcctgca gcaccggttc
     3301 gcacagcagg ctggtgccgg acacgatcag cggctttttc gcgccctgca gcgcatcggc
     3361 aattttctgc gcccgttgca gcagggcggc gtcggcgcct tcaaccgctg gcgcatcggc
     3421 attgatggcg tgcgccaccg caaagccaag gcgggcgata tcttccggcg aaccgtggaa
     3481 atggtcggcg ccgatgtcgt ccagacgggt ggcggacagc gacgcaatga acaccggact
     3541 cagttcacgc tggccaatgt tctggatcgc ttcagcctgc cactcgaaca ccttgcgctc
     3601 ggcggccatc tgacgcgctt tgttcttcgc tgcctgacgc agggccaggg cgatgcgtgg
     3661 tgcagtctgg gtgacatcct cgcccagcac cagcacggcg tcgtattttt ccatgtcgcg
     3721 cagggacggg gttttcaccg gaccttcccg caggattttc accgtggttt tcagcagctg
     3781 gttttcaatg ctgttcacgc cgctgaagaa attgcctgca cccaccagcg attgcagggc
     3841 gaagttgctt tccaggctgg cgcggggcga accgataccg atcacccgct tgctgccccg
     3901 cagggctgcc agggcggcat ccagtgcctg atcctgggtc aggtttatcc aggcctggcc
     3961 gcttttctgt tgcggctggc gcggacggtc ggaacggttc acatagccgt aaccgaagcg
     4021 gccacggtcg cacaggaaat agtggttgat ggtgccgtta tagcggttct cgatgcggcg
     4081 cagttcgccg tagcgttcac ccgggctggt gttgcagccg acgctgcact gctggcacac
     4141 gctggg
//
LOCUS       DASUEG010001001         7706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_103968, whole genome shotgun sequence.
ACCESSION   DASUEG010001001 DASUEG010000000
VERSION     DASUEG010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103968"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VFX11_18780"
     CDS             complement(<1..1429)
                     /locus_tag="VFX11_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4940727.1"
                     /translation="MMTSTLSLRGRLFAGFLLVTLISTLAFALGASYFAKRSLQESID
                     TRLNTAAQAVPGLVEDAYWNIRHQSEAVDDAKYLQQLRALRRYTDSVGLDYLYVMMVD
                     GDKVHMVLDSATEEEVQADDYSHYFEHYSDASDFVLEAHRSGQPQIDEYTDRWGTFRS
                     LFLPVTLGDQRFVIGADITTASIDNAVQQQLMLYGLIALGIFVLGLVLASWLSRLIAR
                     PLQEMMEVTRQVSERRDLTRTIATRGQDEMSAAILGLNQLVAFFRNTLLLVRKEASGS
                     ETLASHLQQTSHTWLQRFETSVDKLATITRQANSIHADTEQASHLVSSARSGMQAAVE
                     DLKHMRQSLEQMSAGVDSNARSGATLAQQLSSLNQQANDISSVLVVIRQIAEQTNLLA
                     LNAAIEAARAGDQGRGFAVVADEVRKLAIETQETLGRTNEGVQRIISSINEAAGNTEH
                     NARIAQDVVSISGTAVSGVDGIASRI"
     gene            complement(1499..3994)
                     /locus_tag="VFX11_18785"
     CDS             complement(1499..3994)
                     /locus_tag="VFX11_18785"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940728.1"
                     /translation="MCAISTETRCLPIPPFLLWLLLLLSCNSASASLLTLDTSETGRQ
                     FPIERHVSFLEDARGELRPEALLRGERDRMLQENQGRNFSFGISSSTYWLMFELSNNG
                     LGDSSASMQRLLEVNYPPLDRITLYFFDSQGNLRQIESGDTLPFDSRPMAHTGYVFPL
                     DINLGETRRFLLKVNSQGSVRVPMTLWEPDYFHEQTRSLMLLHGIYFGIVALMFAYNL
                     ILYLFIRDQTYLYYIVYIAALTFFQSNYSGFAFQYLWPTQPQLNNLIIIYSILMVQIF
                     ATQFTRRVLDTATTQPRLDQIIRWLGTATITLMLLTPILSYHLMLYSGLITAMINIAV
                     IVTAASTNAARGMRTARLFLTAWSAFLLGAVILGGVAVGLLPANGFTNNALIIGSAIE
                     VTLLSLSLADRMNQIERERTRAEQEAKNALMAVNRALLESNKMKDEFLNTISHEMRTP
                     MHGVLSSIDHIRHQDESRKREQFVDSAERSAQQMMMLIDSVLNYTELHSSSFTLEQGT
                     FILSRLAASLDELFRKQAEAKGLQFNIEIQPDVPDLLIGDFRRTQQILMNLLSNAVKF
                     THQGFVRLTIALVTIDERRCRAKIEFRVSDSGIGMLEGTESIIFDRFRQLDSSFSRGF
                     GGLGIGLATSKQIARLMQAELGFQSRPNEGSTFRLVAEFPFSPKNSARKKAEALQRQW
                     QDLARDRLALIVEDNPVNQMVLQASLTKLGLRTRVAGNGNEAISQLLAHPVDIVLMDC
                     QMPVLDGFEATRAIRQLANDKSQVPIIAITANAMSKDRERCVEAGMNDYMSKPVNMHE
                     LKEKLLKWLPLQPRDNTGHGNPVQLGDSDAPST"
     gene            complement(3999..6332)
                     /locus_tag="VFX11_18790"
     CDS             complement(3999..6332)
                     /locus_tag="VFX11_18790"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940729.1"
                     /translation="MDIGDLPPFARVDGLFWHLEDPAGELGIDDLIDPARSLPFQPGT
                     DTALNKGFSQSAFWLVGRFRAAPDATNEKALRYLEVDYSLLDDIRYYVVVDGKVQQRW
                     LTGDTRPFDTRPIDHSRFLFPLELRPGEEKTVLFRVQSTSSLRMPLSFWDPFQFYHTQ
                     RIHLLIDGIYYGIIFLMFFYNLCLLITVRDISYFYYIGYTFSLALFQLGMTGYGFEYL
                     WSRLPVVNSFLIPFSICLIAASILKFSQRVLDLKKNAPHTHRFFNWVVLLQVAGMVAS
                     LSLSYQLVIKPVIWCAMVVASAMLFVSAAEAWKGSHTARLFLIAWASLLLGSMILAFA
                     SMGWIPANLVTTNAFMLGSAIEVTLLSITLAERLNQINRQKADMEKEAKRALEIVNQT
                     LQDNNRIKDEFLATISHELRTPMNGVLGCLQHIHDEPISDKASQYLNYADRSARHMML
                     LVDSLLTYTELQSGKLVLDNRPFTTRELLASARSLFTELSQKKGIALNFFIEESTPDV
                     LISDCVRLSQILNNLLDNAIKFTRHGRVEVRIQGESANPASTDFQLIMQVSDTGIGIA
                     EDKLALIFERFRQVDGAFNRGYGGLGIGLAVIKSLLGFLGGAIAVESTPGKGTTFTVH
                     IPCHRQPAVAEEADVVAPERSPASLRILVVEDNPVNQLVLKGLLQKQGYSVVTADNGI
                     QAVQLVQSTPVDAILMDCQMPELDGFEATRQIRALPLPVSKVPIIAVTANAMSQDRER
                     CVEAGMNDYTSKPIDARALNKKIIYWVNLPGKTQKAG"
     gene            complement(6533..7078)
                     /locus_tag="VFX11_18795"
     CDS             complement(6533..7078)
                     /locus_tag="VFX11_18795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4940730.1"
                     /translation="MKYCSHCGHPVSLKVPEGDNRERHVCDNCGTVHYQNPRIIAGTL
                     PVFGDRVLLCRRAIEPRLGYWTLPAGFMENGETTEQAAARETFEEADAEVDIHGLYTV
                     FNLPHISQVYLFFRADVIDGRHGPGTESLESRLFEEHEIPWQDLAFPTVARTLQYFFS
                     DRRAGEFPVRVEDITAYQRKP"
     gene            complement(7256..7597)
                     /locus_tag="VFX11_18800"
     CDS             complement(7256..7597)
                     /locus_tag="VFX11_18800"
                     /inference="COORDINATES: protein motif:HMM:NF022777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HEX4940731.1"
                     /translation="MRYVLPEFDQLREMAQHDPEGLERLRVQLCDQLIQSAPEAYRRK
                     LRGLQFRVDMERRRAKTPMAACIAISGMMHDSFDRLRQVLNEAVSNASSTTASQADAA
                     TAAKVLPFRRA"
BASE COUNT         1551 a         2182 c         2409 g         1564 t
ORIGIN      
        1 caatgcggct ggcgatgccg tccacgccag acacagccgt gccggaaatg gagaccacat
       61 cctgcgcgat gcgggcattg tgttcggtat tgcccgctgc ttcattgatg ctggaaatga
      121 tgcgttgcac accttcgttg gtgcggccga gggtttcctg ggtttcgatg gcgagcttgc
      181 gcacttcgtc cgccaccacg gcgaaaccac ggccctgatc gccagcgcgg gccgcttcga
      241 tggcggcatt gagtgccagc aggttggtct gctcggcgat ctggcggatc accaccagca
      301 ccgaactgat gtcgttggcc tgctggttca gcgaagacag ctgttgtgca agggttgctc
      361 cgctgcgggc attgctgtcc acgcctgcgc tcatctgctc cagtgactgc cgcatgtgtt
      421 tcaggtcttc cacagctgcc tgcatgccgg accgggccga actgaccagg tgcgatgcct
      481 gttcggtgtc ggcatgaatc gagttggcct gccgggtgat ggtggcgagt ttgtccacgc
      541 tggtttcgaa ccgttgcagc caggtgtgcg aggtttgctg cagatggctg gccagtgttt
      601 cgctgccgct ggcttccttg cgcaccagca gcagggtgtt gcggaaaaag gccaccagct
      661 ggttcaggcc caggatggcg gcggacattt catcctgtcc gcgggtggcg atggtgcggg
      721 taagatcacg tcgctccgac acctgccgcg tcacttccat catttcctgc agcggccgcg
      781 caatcagacg cgacagccag gacgccagca ccaggcccag cacgaaaatg cccagcgcga
      841 tcaggccgta caacatcagt tgctgctgca cggcgttgtc gatggatgcg gtggtgatgt
      901 cggcgccgat cacgaagcgt tgatcgccca gtgtcaccgg caggaacagc gagcggaagg
      961 taccccagcg gtcggtgtat tcatcgatct gcggctggcc gctgcgatgg gcttccagca
     1021 cgaaatcgct ggcgtcggaa tagtgctcga aatagtggct gtaatcatcg gcctgcactt
     1081 cttcctcggt ggcggaatcc agcaccatgt gaaccttgtc gccgtccacc atcatcacgt
     1141 acaggtaatc caggcccacg ctgtcggtat agcggcgcag cgctcgcagc tgctgcaggt
     1201 attttgcgtc gtcaacggct tctgattggt gccggatgtt ccagtacgcg tcttccacca
     1261 gcccgggtac ggcctgggcg gcggtgttca gccgcgtgtc gatgctttcc tgcagggaac
     1321 gtttggcgaa atagctggcg cccagggcaa aagccagcgt ggaaatcagg gtgaccagca
     1381 ggaagccggc aaacaggcgt ccgcgcaggg aaagggtgct tgtcatcatg gcagagggga
     1441 atcggttggc gatgtttcca ttcagtatag acagcttttg ccacaggatg gtgcgggttc
     1501 aggttgaggg ggcgtcgctg tcgcccagtt gaaccggatt cccgtggcct gtgttgtcgc
     1561 gtggttgcag gggcagccac ttcaacagct tttccttcag ctcatgcatg ttcacgggtt
     1621 tgctcatata gtcgttcata ccggcttcca cgcagcgctc ccgatccttc gacatggcat
     1681 tagcggtaat ggcgatgatc ggcacctggc ttttgtcgtt ggccagttgg cggatggcgc
     1741 gggtggcctc gaagccgtcc agtaccggca tctggcaatc catcagcacg atgtccaccg
     1801 gatgggccag cagttgcgag atggcttcgt taccattgcc ggccacccgc gtgcgcaatc
     1861 cgagtttggt cagggatgcc tgcagcacca tctggttcac cggattgtct tccacgatca
     1921 gcgccagccg gtcccgggcc aggtcctgcc actgacgctg caaggcttct gcttttttgc
     1981 gcgcagaatt tttgggcgag aagggaaatt cagcgacaag cctgaaggtg gagccctcgt
     2041 tggggcgcga ctggaaaccc agctccgcct gcatcagccg tgcgatctgc ttgctggtgg
     2101 ccaggccaat gccaagcccg ccaaagcccc gactgaagct gctgtccagc tggcggaagc
     2161 ggtcgaagat gatggactcg gtgccttcca gcatgccgat gccggaatcg ctgacccgga
     2221 attcgatctt cgcgcggcag cgtcgctcat cgatggtgac cagcgcgatc gtcaggcgga
     2281 cgaatccctg gtgagtgaac ttgaccgcgt tgctgagcag gttcatcaga atctgctggg
     2341 tgcggcgaaa atccccgatc agcaggtctg gtacgtccgg ttgaatctcg atgttgaact
     2401 gcagcccctt ggcttctgcc tgcttgcgga acagctcatc cagcgaagcg gcgagccggg
     2461 acaggatgaa cgtgccctgc tcgagcgtga aggacgacga atggagttcg gtgtagttca
     2521 gcacgctgtc gatcagcatc atcatctgct gcgcggagcg ctcggcgctg tccacgaact
     2581 gttcgcgctt gcggctttca tcctggtggc ggatatgatc aatgctgctc agcacgccat
     2641 gcatgggcgt gcgcatctcg tggctgatgg tattgagaaa ttcatccttc atcttgttgc
     2701 tttccagcag cgcgcggttc actgccatca acgcattctt ggcttcctgc tcagcacggg
     2761 tacgttcccg ttctatctgg ttcatgcggt ctgccagtga cagtgacaac agggtcactt
     2821 caatggcgga accgatgatc aacgcattgt tggtgaaacc gttcgccggc agcaggccca
     2881 ctgcaacacc gcctagaatc accgcaccca gcaggaaggc agaccacgcc gtcagaaaca
     2941 ggcgtgcggt gcgcatgcca cgggccgcgt tggtgctggc cgcggtgacg atgaccgcga
     3001 tgttgatcat ggctgtgatc agcccagagt acagcatcag gtggtagctc aggatgggtg
     3061 tcaacagcat cagcgtgatg gtggcggtgc cgagccagcg aatgatctgg tccaggcgtg
     3121 gctgggtggt tgcggtatcc agcacccgtc gcgtgaactg ggtggcaaaa atctgcacca
     3181 tcaatatcga atagatgatg atgagattgt tcagttgcgg ctgcgttggc cacaggtact
     3241 ggaaggcaaa accactgtag ttggactgga agaaagtcag cgctgcgata tagacaatat
     3301 agtaaagata cgtctggtcc cggatgaaca ggtagagaat caggttgtag gcaaacatca
     3361 gcgccacgat gccgaaatag ataccgtgca acaacatcag cgagcgtgtc tgctcatgaa
     3421 aatagtccgg ttcccacaat gtcatgggca cgcggacaga gccctgtgaa tttaccttca
     3481 gcagaaagcg ccgggtttca cccaggttga tgtcgagagg aaagacataa ccggtgtggg
     3541 ccatggggcg gctgtcgaag ggcagggtgt cgccggattc gatctggcgc agattgccct
     3601 ggctgtcaaa aaaatacagc gtgatacggt ccagcggcgg gtagttgact tccagcagcc
     3661 gctgcatgct ggcggaactg tcgcccaggc cgttgttgct gagttcgaac atcagccagt
     3721 aagtggacga ggaaataccg aagctgaaat tccggccctg attttcctgc agcatccggt
     3781 cgcgttcgcc gcgcaacaat gcttccggac gcaattctcc gcgtgcatct tccagaaagc
     3841 tgacatggcg ttcaatcgga aactggcggc cggtttcact ggtgtccagg gtcagcaggc
     3901 tggcgctggc agagttgcag gacagcagca acagcaacca cagcagaaat ggtggaattg
     3961 gcagacagcg cgtttcagtc gagatggcgc acatggcctc agccggcctt ctgcgttttg
     4021 ccgggcagat tcacccagta aatgattttc ttgttcagcg cgcgggcgtc gatgggtttg
     4081 ctggtgtaat cgttcatgcc ggcttccaca cagcgctcgc gatcctgcga catggcgttg
     4141 gcggtgacag caatgatcgg aaccttgctc actggtagcg gcagggcgcg aatttgccgg
     4201 gtcgcctcga agccgtccag ttccggcatc tggcaatcca tcagaatggc gtccaccggc
     4261 gtcgactgca ccagttgcac tgcctggatg ccgttgtcgg cggtgacaac agagtaaccc
     4321 tgcttctgca gcagtccttt cagcaccagc tggttcaccg ggttgtcttc caccaccagt
     4381 atgcgcaggc tggcgggcga gcgctccggc gcgacgacgt cggcttcttc cgccactgcc
     4441 ggctgacggt gacagggaat gtggacggtg aaggtagtgc ccttgcccgg tgtggattcc
     4501 acggcaatcg ccccgcccag gaaacccagc agggacttga ttactgccag gccgatgccc
     4561 aggccgccgt aaccgcgatt gaaggcacca tccacctgac ggaagcgctc gaaaatcagc
     4621 gccagtttgt cctcggcgat gccgatcccg gtgtcgctca cctgcatgat cagttgaaaa
     4681 tcggtactgg ctggattggc ggattcaccc tggatgcgca cttccacccg gccatggcgg
     4741 gtgaatttga tcgcgttgtc cagcaggttg ttgaggatct ggctcagccg cacgcagtcg
     4801 ctgatcagca catccggtgt cgattcctcg atgaaaaaat tcagtgcgat gccttttttc
     4861 tggctcagct cggtgaacag gctgcgtgcg ctggcgagca gttcgcgggt ggtgaagggc
     4921 cggttgtcca gcaccagttt gccggattgc agttcggtgt aggtgagcag gctgtccacc
     4981 agcagcatca tgtgccgggc cgagcggtcg gcataattca gatactggct ggccttgtcg
     5041 gaaatgggtt cgtcgtgaat atgctgcagg caacccagca caccattcat cggcgtacgc
     5101 agttcgtggc tgatggtggc gaggaattcg tccttgatgc ggttgttgtc ctgcagggtt
     5161 tgattcacga tttccagcgc gcgcttggct tccttctcca tgtcggcttt ctgccggttg
     5221 atctggttca ggcgttccgc cagcgtgatc gacagcagcg tgacctcgat ggcggagccg
     5281 agcatgaacg cattggtggt gaccaggttg gccggaatcc agcccatcga cgcaaacgca
     5341 agaatcatcg aacccagcag cagcgatgcc caggcgatca gaaacaggcg tgcggtgtgc
     5401 gaacctttcc aggcctcggc cgcgctcacg aacagcatgg ccgacgccac caccatcgcg
     5461 caccagatca ccggcttgat caccagttga tacgacagcg acagactcgc caccatgccc
     5521 gccacttgca gcagcaccac ccagttgaaa aaacggtgcg tgtggggcgc attttttttc
     5581 aggtccagca cccgctggga aaatttcagg atagacgccg caatcaggca gatgctgaac
     5641 ggaatcagaa aactgttcac caccggcagc cggctccaca ggtattcgaa gccgtaaccg
     5701 gtcatgccca gctggaacag cgcaagggaa aaggtgtaac cgatgtagta aaagtagctg
     5761 atgtcccgca ccgtgatcag caggcacagg ttgtagaaaa acatcaggaa aatgatgccg
     5821 taataaatgc cgtcgataag caggtgaatg cgctgggtgt gatagaactg gaacgggtcc
     5881 cagaacgaca gcggcatgcg cagggaactg gtgctctgga cgcgaaacag aacggtcttt
     5941 tcctcgcccg gacgcagttc cagcggaaac aggaagcggg aatggtcgat cggacgggtg
     6001 tcgaacggcc gggtgtcacc ggtcagccag cgctgctgca ccttgccatc caccaccacg
     6061 taatagcgga tgtcgtccag cagggagtag tccacttcca gatagcgcag tgccttttca
     6121 ttggtggcgt caggcgcggc gcggaaacgc cccaccagcc agaaggcgga ttggctgaat
     6181 cccttgttga gtgcggtatc ggtgccgggc tggaacggca aggagcgggc agggtcgatc
     6241 aggtcatcga tgccgagttc gccggcggga tcttccaggt gccagaacaa tccatccacc
     6301 cgcgcaaacg gtggcagatc gccaatgtcc atggcggcgc ctgcaggcag ggcaaggaca
     6361 ccggccggca tcaggcacca ggccagcaac caggctaaac agacgcgcaa acgcaacatt
     6421 gcaccccgga attgatggaa aacagcggca ctgctcattt cagtctagca gcggggtgcg
     6481 gcgttttccg gccgggggcc aaagaagtat tttgcgggct gcggggcgtc gcttatggct
     6541 ttcgctgata agccgtgatg tcttccaccc gtaccggaaa ctctccggcg cggcggtccg
     6601 agaaaaagta ttgcagggtg cgggccacag tgggaaacgc cagatcctgc caggggattt
     6661 catgttcctc gaacaggcgg ctttccaggc tctcggtacc cgggccgtgg cggccatcga
     6721 tcacatcggc gcggaagaac aggtagacct ggctgatatg gggcaggttg aacacggtat
     6781 acagaccgtg aatgtccact tccgcatcgg cctcctcgaa ggtttcgcgg gcggcggcct
     6841 gttcggtggt ttcgccgttc tccatgaagc cggcgggcag ggtccagtag cccaggcggg
     6901 gctcgatggc gcggcggcac agcagaaccc ggtcgccaaa caccggcagg gtgccggcga
     6961 tgatgcgcgg attctggtaa tggacggtgc cacagttgtc acagacgtgg cgttcccgat
     7021 tgtcgccctc ggggactttc aggctgacag ggtgtccgca atgactgcaa tatttcatgg
     7081 tgtcgcgccg tttcgctgcg ggaaagcggc agtataaacg caggcgccaa aacccgatac
     7141 cggtcaggcc ggggtgtgca ggcaaaaagc aggcaaaaaa aagggccctt gaggggcccc
     7201 gcactatgct tttgaaagga agtgctctgc aaagaagaaa agcggaaatc ggctgtcagg
     7261 cgcggcggaa gggcagtacc ttcgcggcag tggccgcatc agcctggctt gcagtggtcg
     7321 atgaggcatt gctgacggcc tcattgagaa cctggcgcag gcggtcgaac gaatcatgca
     7381 tcatgcccga gatggcaatg caggccgcca tgggcgtctt ggcgcggcgg cgttccatgt
     7441 ccacccggaa ctgcaggcca cgcagtttgc ggcggtatgc ttcaggagca ctctggatca
     7501 actggtcgca cagctgcacc cgcaaccgct ccagcccttc cggatcgtgc tgtgccatct
     7561 ctctcaactg atcaaattcc ggtaacacgt aacgcatcga aattacctct taggcctgcc
     7621 aatcacgccg ggtgtattgg cgtcccgccc cccaaatgtg agaactcgac aatcgtgtgg
     7681 aatcgcaaga atcctaatta agttat
//
LOCUS       DASUEG010001002         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_147995, whole genome shotgun sequence.
ACCESSION   DASUEG010001002 DASUEG010000000
VERSION     DASUEG010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147995"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1791
                     /gene="yidC"
                     /locus_tag="VFX11_18805"
     CDS             88..1791
                     /gene="yidC"
                     /locus_tag="VFX11_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4940732.1"
                     /translation="MDWQRTLIIAALAVVSYLMILQYQKDYIDTPAAIQAETSAQTAA
                     SADQVPTPDSASAPPSAASADVPQPAPTAQSQAVPVTQPAGQSQLIEVETDLLQARID
                     PVGGDIVSLALKAYPVSIAQPNQPFQLLRQDGQQFYIAQSGLVGAQGTDSAAGRPRYV
                     SSKSQYRMDGDSLVVDLTYNQDTGAQITKTFTFQRGSYLIDVNTRVDNRGASPWQAGM
                     YGQIQRDGSPDPGTNTGAALGMITFLGAAYWDQEKPYNKIALEDIGSTALDKPIPGGW
                     LAMIQHYFLSAWIPDQNVTHHYNTRKTQGRDNYIISFTTPAVNVAPGASHTFSAKFYA
                     GPKIQKNLEAILPDKGLDLAIDYGPLFFISKLLFWLLSFFHGLTANWGVAIILLTFVV
                     KLIFLYPSAISYRSMAKMRKVQPLLARLKEQFGDDRQKMSVEMMNIYKREKINPLGGC
                     LPILIQMPVFLALYWALQESVELRQAPFFFWIKDLSVMDPWFVLPLIMGGTMFIQQML
                     NPAPTDPMQARVMKFMPVIFTVMFLFFPAGLVLYWVTNNTLSILQQWLITRSIEAGDK
                     K"
     gene            1849..3243
                     /gene="mnmE"
                     /locus_tag="VFX11_18810"
     CDS             1849..3243
                     /gene="mnmE"
                     /locus_tag="VFX11_18810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX4940733.1"
                     /translation="MNLPTAIDTIAAQATPSGRGGVGIIRISGPHTVAIAQALCQRAS
                     AFTPRFAHFCRFHNAQQQVLDEGLLLYFPAPNSFTGEDVVEIQGHGGPVVMASLLNRV
                     VELGARVARPGEFSERAFLNDKIDLAQAEAIADLIDSASEQAARSALRSLQGAFSDRI
                     NVLLEQLIALRIYVEAAIDFPEEEIDFLGDGKVLQDLDAIRAQLQQVLQEASRGVLLK
                     EGMKVVLAGKPNAGKSSLLNALSGRDSAIVTAIAGTTRDVLREHIHLDGMPLHITDTA
                     GLRDSPDEVEQEGIRRAWSEIRAADRVLMLVDARDLFSGATEIEMEPEKIWPEFVHEL
                     PDRARLTLVINKIDLVNQTPRALAAGMTPVIFLSAKNGSGVDLLREHLKTVMGYGGEG
                     DSSFTARQRHIDALKKAQQAIEQGRAQLVQSGAGELLAEDLRLAQEYLGEITGRFTPD
                     DLLGRIFSSFCIGK"
     gene            3268..4338
                     /locus_tag="VFX11_18815"
     CDS             3268..4338
                     /locus_tag="VFX11_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940734.1"
                     /translation="MKITKDKKSKQKAKQIGGMAGRFVQLQEDIRKKRQRNASLRMDI
                     DALLAEYQNRILPHERRLVQPTSALLQRLIDFFAMKSLTRWQRDELVVWIHETLELLG
                     RLDSEAAQTMGKAFNQKLADYFNMSVEQLDKIQAEEDDIEAIVEQLFREMNPDDGDED
                     VDLQDDLFGFDESEPAAGQNENPEPVVSQKPAPAFGEEWIKSLFRRTAKILHPDREQD
                     EQRRNEKHILMSQLLTARDAGDVMAMIELYTTHVADAQAEFTEAEYQDILVMLEDQLQ
                     RLDEESDDIVNATPFHHFLYRRLYSRQKAARERKIKAYLAQIDEDIQAQMEGIAALRT
                     LKDLKMALEVRLSHFDDVDMKW"
BASE COUNT          971 a         1186 c         1284 g          925 t
ORIGIN      
        1 tccctggcat gaaggcggtt atgacccggt tccgggcacg gtcggctccg attccgaaca
       61 atttcccccc aacagtaagt aatcaagatg gactggcaac gcactctgat cattgccgcc
      121 ctggcggttg tttcatattt gatgattctt cagtaccaga aggattacat cgacactcca
      181 gccgccatcc aggccgaaac gtccgcgcaa accgccgcat cggcggatca ggtgccgaca
      241 ccggatagcg catcagcgcc accatcggcc gcttccgccg atgtacccca accagcgccg
      301 acagcacaat cccaggcggt tccggtgaca cagcctgccg gccaatcaca gctgatcgag
      361 gtcgagaccg acctgctgca ggccagaatc gatcctgtcg gcggcgatat tgtcagtctg
      421 gccctgaagg cgtatccggt tagcatcgcc cagccgaacc agccgtttca gctcctacgt
      481 caggatggtc agcagttcta tattgcccag agcggactcg tgggtgcgca aggcaccgat
      541 agtgccgccg gacgcccgcg ctatgtgagc agtaaaagtc agtaccgcat ggacggcgac
      601 agcctggtgg tggacctgac ctacaaccag gacacgggcg cgcagatcac caaaacattt
      661 accttccagc gcggcagcta cctcatcgat gtgaataccc gggtcgacaa ccgtggtgca
      721 tcaccctggc aggccgggat gtacggtcag atccagcgcg acggcagccc cgatccgggc
      781 accaacaccg gcgcagcgct cggcatgatt acgttcctgg gtgccgcata ttgggatcag
      841 gaaaaaccct ataacaagat tgcactggaa gatattggca gcactgcact cgacaagcct
      901 attcctggcg gctggctggc gatgatccag cattatttcc tcagtgcctg gattcccgac
      961 cagaatgtca cgcatcatta caacacgcgc aagacccagg gccgtgacaa ttacatcatc
     1021 agttttacca cgcctgcggt aaatgttgcg cccggagcca gtcatacatt cagtgccaaa
     1081 ttctatgctg gccccaagat ccagaagaat ctggaagcga ttctgccgga caaaggtctc
     1141 gatcttgcca tcgattatgg tccgctgttt ttcatttcca agctgctgtt ctggctgctg
     1201 tcattcttcc atggcctcac cgccaactgg ggtgtggcca tcattctgct gacgtttgtc
     1261 gtcaaactga ttttcctgta tccctccgcc atcagttacc gctccatggc caagatgcgc
     1321 aaggtgcagc ctctgctggc ccgactgaag gaacagttcg gggatgatcg ccagaagatg
     1381 tccgtggaaa tgatgaacat ctacaagcgg gaaaaaatca atccgctggg cggctgtctg
     1441 ccgatcctga tccagatgcc ggtgttcctg gcgctgtact gggcactgca ggaaagcgtg
     1501 gaattgcggc aggcgccgtt cttcttctgg atcaaggatc tgtcggtcat ggacccctgg
     1561 ttcgtgttgc cgctgatcat gggtggcacc atgtttatcc agcagatgct gaatccggca
     1621 cccactgatc caatgcaggc gcgggtcatg aaattcatgc cagtgatttt caccgtgatg
     1681 ttcctgttct tccccgctgg actggtgttg tactgggtga ccaacaacac cttgtcgatc
     1741 ctgcagcaat ggttgattac ccgcagcatc gaggcgggtg acaaaaaata aaaattccgg
     1801 cgccgatggc gccggttttc gttttgacag catcacattg ttattgccat gaatctgcca
     1861 actgccattg acaccattgc cgcgcaagcc acgccttcag gccggggtgg tgtcggcatc
     1921 atccgcattt cgggtccgca tactgttgcc atcgcccagg ccctgtgtca gcgcgcttct
     1981 gccttcactc cccgcttcgc acacttctgc cgttttcata atgcgcagca gcaagtgctg
     2041 gacgaaggct tgctgctgta ctttcccgcg ccgaattcgt tcaccggtga agacgtggtt
     2101 gagatccagg gccacggcgg accagtggtg atggccagcc tgcttaaccg ggtggtggaa
     2161 ctgggggcgc gggtggcacg ccccggcgaa ttttccgagc gcgcgtttct gaacgacaag
     2221 atcgatctgg cccaggcgga agcgattgcg gacctgattg attccgcgtc cgagcaagca
     2281 gcccgcagtg ccttgcgttc tttgcaaggc gccttttctg atcgtatcaa tgtgctgctg
     2341 gagcagttga tcgcgctgcg catctatgtg gaagccgcga ttgattttcc cgaagaggaa
     2401 attgattttc ttggcgacgg aaaagtgttg caggatctcg atgccattcg agcgcagttg
     2461 cagcaagtcc tgcaggaagc cagtcgcggc gtactgttga aggaaggtat gaaagtggtg
     2521 ctggcgggaa aacccaatgc gggcaaatcc agtctgctga acgcattgtc ggggcgcgac
     2581 tctgccattg tgaccgccat tgccggcact acccgcgatg tgttgcgcga gcatattcat
     2641 ctggatggca tgccgttgca tattaccgac acggccggct tgcgcgacag tccggatgag
     2701 gtggagcagg aaggcatccg ccgggcctgg tctgaaattc gcgccgctga ccgggtactg
     2761 atgctggtgg atgcgcgtga cctgttttcc ggtgcaactg aaattgaaat ggaaccggaa
     2821 aaaatctggc cggagttcgt gcatgaattg ccggaccgcg cgcggctgac gttggtgatc
     2881 aacaagatcg atctggtgaa tcagacaccg cgcgcgctag cagcggggat gacgccggtg
     2941 atttttcttt ctgccaaaaa cggcagtggc gtggatctgc tgcgtgagca tctgaaaacc
     3001 gtgatgggat atggtgggga aggcgacagt agttttaccg cccgtcagcg tcatatcgat
     3061 gcgctgaaaa aggcgcagca ggcaattgag cagggcaggg cgcagctggt gcagtccggt
     3121 gctggcgaat tgctggcgga ggatttgcgg ctggcgcagg aatatctggg cgaaattacg
     3181 gggcggttta cgccggatga tctgctgggg cggatttttt ccagtttctg tatcggcaaa
     3241 tgagatggct gtgctcggaa taacgtcatg aaaatcacca aggacaaaaa atcaaagcag
     3301 aaggccaaac agatcggagg aatggcagga cgctttgtgc agctgcagga agacatccgc
     3361 aagaaacgcc agcgcaacgc atccctgcgg atggatatcg atgcgctgct ggcggaatac
     3421 caaaaccgta tcctgcccca tgaaaggcga ctggtgcagc cgacgtcggc gctgctgcaa
     3481 cggttgatcg attttttcgc catgaaaagc ctgacccggt ggcaacggga tgagctggtg
     3541 gtctggattc acgagacgct ggaattgctg ggtcgtctgg acagcgaagc cgcgcagacg
     3601 atgggcaaag ccttcaacca gaagctggcc gattacttca atatgtccgt tgagcaatta
     3661 gacaagattc aggccgaaga ggacgacatc gaggccatcg tcgagcagct gtttcgcgaa
     3721 atgaatccgg atgacgggga tgaagacgtc gatcttcagg atgatctgtt cggttttgat
     3781 gaatctgaac cggccgccgg gcaaaatgaa aatccagaac cggttgtctc gcaaaagccg
     3841 gccccggctt ttggtgagga gtggatcaaa tccctgtttc gccgcaccgc caaaatcctg
     3901 caccccgatc gcgagcagga tgaacaacgc cgcaacgaga aacacattct gatgtcgcaa
     3961 ttgttgacag cgcgggatgc gggtgacgtc atggccatga tcgagttgta caccacccat
     4021 gttgccgatg cgcaagcgga attcactgag gcggaatacc aggacatcct cgtcatgctg
     4081 gaagatcaat tgcagcggct ggatgaagag agcgacgaca ttgtcaatgc cacgcccttc
     4141 catcatttcc tgtaccggcg cctgtactcc agacagaagg cggcgcggga acggaaaata
     4201 aaggcctatc tggcgcagat cgatgaggat attcaggcgc agatggaagg catcgcggcg
     4261 ctgcggacgc tgaaggatct gaaaatggcg ctggaagtcc ggctgagcca ctttgatgac
     4321 gttgatatga agtggtaaag ggtggtggat cacgccgggt ttacgg
//
LOCUS       DASUEG010001003         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1848524, whole genome shotgun sequence.
ACCESSION   DASUEG010001003 DASUEG010000000
VERSION     DASUEG010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848524"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="alr"
                     /locus_tag="VFX11_18820"
     CDS             complement(<1..661)
                     /gene="alr"
                     /locus_tag="VFX11_18820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX4940735.1"
                     /translation="MSRGTRAVIDLRALQHNFRLVRERAPGSQVLAVVKADGYGHGAV
                     AVAQALREADAFGVATFGEALVLQRAGITQPILLMEGVMSAEELQLAAQHRFQIVVHQ
                     FWQLALLQRVAVSQPLVVWLKVDTGMHRLGIPLTACTQVWDQLRACASVNEIRLMSHL
                     ACADDADHPLNRVQREGFAALVHTTGAVQSSLANSAGLLRGEAFQFQWVRPGLMLYGA
                     TP"
     gene            complement(667..2055)
                     /gene="dnaB"
                     /locus_tag="VFX11_18825"
     CDS             complement(667..2055)
                     /gene="dnaB"
                     /locus_tag="VFX11_18825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX4940736.1"
                     /translation="MSDFPIELQLDSDIETASIKVPPHSIEAEQSVLGGLMLDNNAFD
                     DVAELVTDVDFYRRDHRLIFRAISQLAELSKPFDVVTLGEALDNAGILDESGGMAYLA
                     LLAKNTPSASNIKAYADIVRERSVLRQMIQVSNEIAESAFRPEGRSSSELLDEAERKV
                     FNIAEQGSKGAGPVPIKQVLKATVDRIDQLFKSDDKITGIPTGFTDLDEKTSGLQGGA
                     LVIVAARPAMGKTTFSMNMAENALIRTGKPVLVFSMEMPAEQIVMRMLASLGRIDMSR
                     VTSGKLEEDDWPRFSSAVSLLAEQKLFIDDSAGLSPVELRARARRVARDQGDLGLIMV
                     DYLQLMQVPGAENRVNEISEISRSLKGLAKEMNCPVVALSQLNRGLEQRPNKRPVMSD
                     LRESGAIEQDADIIMFIYRDEVYNEDSEDKGIAEIIIGKQRNGPIGTVRLAFLGRYTR
                     FEDLAHNSYGEY"
BASE COUNT          494 a          770 c          684 g          430 t
ORIGIN      
        1 gcggcgtggc gccatacagc atcaggccgg ggcgcaccca ctggaactgg aacgcctcgc
       61 cccgcaacag accggccgaa ttggccaggc tgctttggac tgcgcccgtt gtgtgcacca
      121 gcgcggcaaa accctcccgc tgaacgcggt tgagggggtg gtctgcgtca tccgcgcagg
      181 ccagatggct catcagccgg atttcattca cgctggcgca ggcacgcaac tgatcccaca
      241 cctgggtgca agcggtgagc ggaatgccca gccggtgcat gccagtatcc actttcaacc
      301 acaccaccag cggttgcgac accgccaccc gctgcaacaa tgccaactgc cagaactggt
      361 gtaccacgat ctggaaccgg tgctgtgcgg ccagctgcag ttcctcggcg ctcatcacgc
      421 cttccatcag caggatcggc tgggtgatgc ccgcccgctg cagcaccagt gcctcgccaa
      481 aggtcgccac gccgaaagcg tcggcctcgc gcagcgcctg cgccacggcc accgctccat
      541 gcccgtagcc atccgccttc accaccgcca acacctgact gccgggcgca cgctcgcgca
      601 ccaggcggaa attgtgttgc agggcgcgca ggtcgatcac cgcgcgggtg ccgcgactca
      661 tgggggtcag tattctccgt aggaattgtg ggccagatct tcaaagcggg tgtaacggcc
      721 cagaaacgcc agccggacgg tgccgatggg gccgttccgc tgtttgccga tgatgatttc
      781 cgcgatgccc ttgtcttccg aatcctcgtt atagacctca tcacggtaaa tgaacatgat
      841 gatgtccgcg tcctgctcga ttgcaccgga ttcccgcagg tccgacatga ccgggcgctt
      901 gttcgggcgc tgctccagac cccggttcag ctgcgacagc gccaccaccg ggcagttcat
      961 ctcttttgcc aggcctttca gcgaacggga gatttccgaa atctcgttca cccggttttc
     1021 cgcgccaggc acctgcatca gctgcaggta gtccaccatg atcaggccca ggtcgccctg
     1081 gtcccgcgcc acacggcggg cgcgggcgcg cagttcaacg gggctgaggc cggcggaatc
     1141 atcgatgaac agcttctgct ccgccagcag gctcacggcc gaactgaaac ggggccagtc
     1201 atcttcctcc agcttgccgg acgtcacccg cgacatgtcg atgcgtccca gggacgccag
     1261 catccgcatc acaatctgct cggcgggcat ttccatactg aacaccagca ccggtttgcc
     1321 ggtgcggatc agcgcattct ccgccatgtt catcgaaaac gtggttttac ccatggccgg
     1381 acgggccgcc acgatcacca gtgcgccacc ctgcaggccc gaggtctttt catccagatc
     1441 ggtaaacccg gtgggaatac cggtaatctt gtcgtcggac ttgaacagtt gatcgatgcg
     1501 gtcgacggtc gccttcaaca cctgcttgat cggcaccgga ccggcaccct tggagccctg
     1561 ctcggcgatg ttgaacacct tgcgctcggc ctcgtccagc agttcggagg acgaacgacc
     1621 ttccgggcgg aacgccgact ccgcaatctc gttcgacacc tgaatcatct gccgcagcac
     1681 agaacgttca cgcacgatgt cggcataggc cttgatgttg gacgcactgg gcgtattttt
     1741 cgccagcagc gccagatagg ccatgccgcc gctctcatcc agaataccgg cgttgtcgag
     1801 ggcttcgccc agggtcacca catcgaacgg cttgctcaac tctgccagct gcgagatggc
     1861 gcgaaaaatc agccgatgat cgcggcggta aaagtccacg tctgttacca gctcggccac
     1921 gtcgtcaaag gcgttgttgt ccagcatcag accgcccagc acagattgct cggcttcgat
     1981 ggaatggggc ggcaccttga tggacgcggt ttcgatatcc gagtccagtt gcagctcgat
     2041 gggaaaatca ctcatggcag gcgaattcgg acagtaggaa aacgggaaag tggagcaagt
     2101 gtaacggctg gtttgcaaaa cgcgcaacga agttggcagg aagatcaggg ccgttcagcg
     2161 gaatgagcaa gcacagcaga caacagatta cataacgcct gaacagcacg tctcacgcac
     2221 gaacacgcag acctggaaac aaaggcgggg acatccgcac ttactgcgca catgcacttg
     2281 tgaacgagac ataaacaaca aaactccggc ctccacacag gaaaccagag ttttgcaggg
     2341 cgtgacagaa atacctggcc gaagccagtt tattccgg
//
LOCUS       DASUEG010001004         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2135521, whole genome shotgun sequence.
ACCESSION   DASUEG010001004 DASUEG010000000
VERSION     DASUEG010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135521"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFX11_18830"
     CDS             complement(<1..812)
                     /locus_tag="VFX11_18830"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4940737.1"
                     /translation="MQPDRSDHFDCCIIGAGVVGLAIAHELALRKRSVVILEQEDRHG
                     TGSSSRNSEVIHAGIYYPAGSLKATLCVEGREQLYRFCESRGVRHRRIGKLIVAGTQE
                     RHADLELLQKKAAANGVTDLQWIEAKALAQLEPHVSGTSALLSPSTGIVDSHGLMLAL
                     LASIEASGGTLSLKTEFLQAQRRSDGFSIQANSVGESCHFRCEQLVNAAGLRAQAVAA
                     RIDGLDSNAIPPLHLCAGRYFALSGAAPFTRLIYPLPDAAMTGLGIHATLDL"
     gene            complement(827..1849)
                     /locus_tag="VFX11_18835"
     CDS             complement(827..1849)
                     /locus_tag="VFX11_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEX4940738.1"
                     /translation="MVEAGNELAAQWNAYVDAQPRSCVYHRFEFRHIIERSFGHRVLY
                     LAALDQNRRICGILPAIQINSRIFGNYMVSVPFFNYGGALADDDGIEQTLMQHLNERG
                     AALGVSHIEYRDTQPRPGYVQKTEKSSLILKLPSSPDTLWDDIGTKVRAQIKKAQGSG
                     LTFKTGRHELLDDFYHVFAINMRDLGTPVYAKDFFKNILDAATLDARLAIAYLNNKPV
                     SCAFLIAWHDTMEIPWASTLREANACNANMFLYWNVLKSATESGIGFFDFGRSSKDAG
                     TFKFKLQWGAQPQQLYWHYWLKDQTDLPALNPNNPKYRIAIAVWQRLPVWLTRLIGPH
                     LVKNLP"
     gene            complement(1884..>2110)
                     /locus_tag="VFX11_18840"
     CDS             complement(1884..>2110)
                     /locus_tag="VFX11_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940739.1"
                     /translation="ELEKGICSRKIGEAKKAGTPADALLEQIRQVSEKIRSIKSAQDE
                     LNAAASALLTPAATPEEKKTRRFSALLRTR"
BASE COUNT          381 a          626 c          657 g          446 t
ORIGIN      
        1 tgcaggtcca gggtggcgtg gatgcccagc ccggtcatgg cagcgtccgg cagcggatag
       61 atcagccgtg tgaacggtgc ggcaccggac agtgcgaaat agcggcccgc gcacaggtgc
      121 agcgggggaa tggcgtttga atcaagaccg tcgatgcggg ccgccactgc ctgcgcgcgc
      181 aggccggcag cattgaccag ttgctcgcag cgaaaatggc aggattcgcc gacgctgttg
      241 gcctggatgc tgaagccatc gctgcgccgc tgcgcctgca ggaattcagt tttcagactc
      301 agagtgccgc cgctggcctc gatgcttgcc agcagcgcca gcatcaggcc gtggctgtcg
      361 acgatgccgg tggacggtga cagcagcgcg gatgtgccgc tgacgtgcgg ttccagttgc
      421 gcgagtgctt tcgcttcaat ccattgcaaa tccgtcacgc cgttggcggc ggctttcttc
      481 tgcagcagtt ccagatccgc gtgccgttcc tgcgttcctg ccacgatcag cttgccaatg
      541 cggcggtggc gaacgcctcg gctttcacag aagcggtaca actgctcccg gccttccacg
      601 cacaacgtcg ctttcaatga gccggcggga taatagatcc cggcgtgaat cacttcgctg
      661 ttgcggctgc tgctgccggt gccgtgacga tcctcctgct ccagaatcac gacagagcgt
      721 ttgcgcaggg ccagctcgtg cgcaatcgcc agacccacca cgccggcgcc aatgatgcag
      781 cagtcgaaat ggtcggatcg gtcaggctgc atcggcacgc cttcgctcag ggcagatttt
      841 tcacaaggtg cggaccgatc aggcgcgtca gccacaccgg tagccgctgc cacaccgcga
      901 tcgcaatgcg gtacttcgga ttgttcggat tcagcgccgg caaatcggtc tggtccttca
      961 gccagtaatg ccagtacagc tgctgcggct gcgcgcccca ttgcagcttg aacttgaagg
     1021 tgcccgcatc cttggacgag cggccgaaat cgaaaaagcc gatgccgctt tcagtggcgg
     1081 acttcagcac attccagtac aggaacatgt tggcgttgca ggcatttgcc tcgcgcaggg
     1141 tggacgccca gggaatttcc atggtgtcgt gccaggcaat cagaaatgcg caggacaccg
     1201 gcttgttgtt gagataggcg atggccagcc gggcatcgag cgtcgctgca tccagaatgt
     1261 ttttgaagaa atccttcgcg tacaccggcg tgcccagatc gcgcatgttg atggcaaaca
     1321 catggtagaa gtcatccagc agttcatgcc ggccggtttt gaaggtcaga ccgctgcctt
     1381 gcgctttctt gatctgcgca cgcaccttgg taccgatgtc gtcccacagg gtgtcggggc
     1441 tcgatggcag cttcagtatc agcgaggatt tttcggtttt ctgcacatag ccagggcggg
     1501 gctgggtgtc gcggtattcg atgtgggaaa cgcccagtgc ggccccgcgc tcgttcaggt
     1561 gctgcatcag tgtctgttcg atcccgtcat catcggccag agcgccgccg taattgaaaa
     1621 acggcaccga caccatgtag ttgccgaaaa tgcggctgtt gatctggatg gcgggcagaa
     1681 tgccgcaaat gcgccggttc tggtccaggg cagcgagata taaaacccgg tggccgaatg
     1741 agcgctcgat gatgtgcctg aattcgaagc ggtggtacac gcagctgcgg ggctgggcat
     1801 cgacataggc attccactgt gcagccagtt cgttgccggc ttccaccacc tcgatctggc
     1861 ccgctggggt tgtcgcacca acgtcaccgc gtgcgcagaa gtgcgctgaa acggcgggtt
     1921 tttttttctt ccggcgtcgc tgccggtgtc agcaacgcgc tggccgctgc gttcaactca
     1981 tcctgggcgc ttttgatgga acggattttt tccgacactt gccggatctg ctccagcaat
     2041 gcatcagccg gtgtgccggc ctttttggcc tcgccgattt tgcgcgagca gatgcctttt
     2101 tccagctcca
//
LOCUS       DASUEG010001005         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_899591, whole genome shotgun sequence.
ACCESSION   DASUEG010001005 DASUEG010000000
VERSION     DASUEG010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899591"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFX11_18845"
     CDS             complement(<1..714)
                     /locus_tag="VFX11_18845"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4940740.1"
                     /translation="MMRFDYRGRTRQGDLVSGTLDAASAEAAALQLQNQAIVPVAITE
                     AKAASGKSININQLFGPPKVRPDDLVMFCRQMRALTRAGIPIIQAIGGLADISKSVTL
                     KAALSDVSVRLATGSTLANALASHGKIFNSMFVSLIHVGENTGRLDDAFSKLIGHIEL
                     ERETRNRITQAMRYPIAVMSAMVIAMFIVNLFVIPQFAKVFKKVGAELPLPTRVLVAT
                     SEFTVAYWWLILGVLIGSVV"
     gene            complement(739..2463)
                     /locus_tag="VFX11_18850"
     CDS             complement(739..2463)
                     /locus_tag="VFX11_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX4940741.1"
                     /translation="MEPARAPKVRIGDLLVSKGLIIDDQLKRALDEQKRTGKKIGKVV
                     VDLGFVSESQLLQALAAHFSYPFVDMARFRLNNELIKTLPETYARRFRAIVLSEQPDG
                     LLVGMGDPLDLLAMDELQRILRRPVHPAFVKEQELLDAMDRAYRRSEQIASIAVELDS
                     ELGTTDFDLDNLATTTDTAEAPVVRLLQSIFEDAVQVRASDIHIEPDETLLRVRLRID
                     GELQEQVMKEKRIAPALVSRLKIMSGLDISEKRLPQDGRFNIRVTGKSIDVRLSTLPV
                     PYGECVVMRLLDQSAGMLSMDRLGMPDAIRVRFENLIHRPYGMVLVTGPTGSGKTTTL
                     YAALNLLNAPERKVITAEDPIEYRLSRINQVQINSKIGLTFASVLRTALRQDPDIILV
                     GEMRDHETAEIGLRAAMTGHMVLSTLHTNDSVSTAMRLLDMGCDAFLVASSLRAVIAQ
                     RLVRRICEYCKQPYKLTSQEMAWLGNIDSTALDAQFYKGTGCHQCNNTGYSGRIGVYE
                     LLEMDEHLLFALRKGDAAEFTRAAENSAHFRSLAKCALDYARQGVTSVQEVFKISASL
                     DDRNAPVL"
     gene            complement(2441..>3319)
                     /locus_tag="VFX11_18855"
     CDS             complement(2441..>3319)
                     /locus_tag="VFX11_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940742.1"
                     /translation="GYNGELETSDNKIAGGLEQADFSSLVNENVGLIGGVFTLGANFT
                     DFNAVLALLQLQGDVQVLSSPRISTLNNQKAVIKVGNDEFFVTDISSTTTTTSSTVSD
                     TPDITLTPFFSGIALDVTPQISETGDVILHVHPSITEVVEKTKDIGLGEETLSLPLAF
                     SSIRETDSIVRASNGQVVVIGGLMQDSFREEVSKIPWLGDIPVIGDFLFSQHLRRKVK
                     SELVILLKPHVVQSEFYTQQNAAIEERFKPYIPGLNVSPIQKEAEPAQEADPDASVTV
                     TPASSEAAQDGTGTSP"
BASE COUNT          664 a         1037 c          933 g          685 t
ORIGIN      
        1 cacgaccgaa ccgatcagca cacccaggat cagccaccag tacgccacgg tgaattccga
       61 tgtcgccacc agcacgcggg tgggtagcgg cagttccgca cccactttct tgaatacttt
      121 tgcgaactgc ggaataacga acaggttgac gatgaacatc gcaatcacca tcgccgacat
      181 caccgcgatc gggtagcgca tggcctgggt gatacggttg cgggtttccc gctccagttc
      241 gatatggccg atcagtttgg aaaacgcgtc atccagccgg ccggtatttt cgcccacgtg
      301 aatcaggctg acgaacatgc tgttgaaaat cttgccgtga ctggcgagcg cattggccag
      361 cgtcgagccg gtggccagac gcaccgacac atccgacagc gccgctttca gcgtgactga
      421 cttgctgatg tctgccagcc cgccgatggc ctgaataatg ggtatgccgg cacgggtgag
      481 cgcccgcatc tgccggcaga acatcaccag atcgtcgggg cgcaccttgg gcgggccgaa
      541 cagctggttg atgttgatgc ttttgccaga cgccgccttc gcctcggtga tggccaccgg
      601 cacaatcgcc tgattctgca attgcagcgc cgctgcttct gcacttgccg catccagcgt
      661 accgctgacc agatcaccct ggcgggtgcg gccccggtaa tcaaaccgca tcatgaccgc
      721 acacctgccc ggcgcttact acaacaccgg cgcattgcga tcatccagcg atgccgagat
      781 cttgaagact tcctgcaccg acgtgacacc ctgccgcgca taatccagcg cgcatttcgc
      841 cagggaacgg aaatgggcac tattttctgc tgcccgggtg aattcggcgg catcgccctt
      901 gcgcagcgcg aacagcagat gctcatccat ttccagcagc tcatacacac cgatgcggcc
      961 ggaatagccg gtgttgttgc actgatggca tcctgtaccc ttgtagaact gggcgtcgag
     1021 ggcagtactg tcgatattgc ccagccaggc catctcctgc gacgtcagct tgtagggctg
     1081 cttgcagtat tcacagatgc gacgcaccag gcgctgggca atcaccgccc gcaacgacga
     1141 cgccaccaga aacgcgtcgc accccatgtc gagcagacgc atcgctgtgg aaaccgaatc
     1201 attggtgtgc agggtggaca gcaccatatg gcccgtcatc gcggcccgca agccgatttc
     1261 agcggtctcg tgatcgcgca tctcgcccac cagaatgatg tccgggtcct gccgcaatgc
     1321 cgtgcgcagc accgacgcaa acgtcaggcc gatcttgctg ttgatctgca cctggttgat
     1381 gcgcgacaga cgatattcaa tgggatcttc cgccgtgatg actttgcgct ccggcgcatt
     1441 caacagattc agcgccgcat acaatgtggt ggttttgccg ctgccggtgg ggccggtcac
     1501 cagcaccatg ccataggggc ggtgaatcag attctcgaac cgcacccgga tagcgtccgg
     1561 catgcccaga cggtccatcg acaacatgcc cgccgactga tccagcagac gcatgaccac
     1621 gcactcgcca tacggcaccg gcaaggtgga aagacgcacg tcgatactct tgcccgttac
     1681 ccggatgttg aaccggccgt cctgcggcaa gcgtttttcc gaaatatcca ggccggacat
     1741 gattttcagg cgcgacacca gcgcaggcgc gatgcgcttt tccttcatca cctgttcctg
     1801 cagttcgcca tcgatgcgca ggcgcacccg cagcagggtt tcgtccggct cgatgtgaat
     1861 atccgaggca cgcacctgca ccgcatcttc aaaaatactt tgcagcagac gcacgacggg
     1921 cgcttcggcg gtgtcggtgg tggtagccag attatcgagg tcgaaatccg tggtgcccag
     1981 ttcggaatcc agttccaccg caatcgatgc gatctgctcg gaccggcggt aggcgcgatc
     2041 catcgcatcc agcagttcct gctccttgac gaacgccggg tgcacgggcc gccgcagaat
     2101 ccgctgcagt tcatccattg ccagcagatc cagcgggtcg cccatgccca ccagcaggcc
     2161 gtcgggctgc tcactcagca cgatagcgcg gaaacggcgc gcataggttt ccggcagcgt
     2221 tttgatcaat tcattgttga ggcggaaccg cgccatatcc acgaatggat agctgaaatg
     2281 ggcagccagg gcctgcagca gctggctctc gctgacaaaa cccagatcca ccaccacctt
     2341 gccgattttc ttgccggtgc gtttctgttc gtccagcgcg cgcttgagct gatcatcgat
     2401 gatcagccct ttcgatacca gcaggtctcc aatgcggact ttaggggctc gtgccggttc
     2461 catcttgcgc ggcctcgcta ctggcagggg tgacggtgac ggatgcatcc gggtcagctt
     2521 cctgcgccgg ctctgcttcc ttctggatcg gcgacacgtt caggcccgga atatagggtt
     2581 tgaagcgttc ctcgatggcg gcgttctgct gggtgtagaa ttcactctgc accacatgcg
     2641 gcttcagcag aatcaccagt tcacttttca ccttgcggcg caggtgctgg ctgaacagga
     2701 aatccccgat tacgggaatg tcacccagcc agggaatttt cgacacctcc tcgcgaaagc
     2761 tgtcctgcat caacccaccg atcaccacca cctgaccgtt gctggcacgt acgatgctgt
     2821 cggtttcgcg aatggaactg aacgccaggg gcaacgacag ggtttcttct cccaggccga
     2881 tatccttggt cttttccacc acctcggtaa ttgaggggtg cacatgcagg atcacgtcac
     2941 ccgtttcgga aatctgtggc gtcacatcca gggcgatgcc ggagaaaaaa ggcgtcaggg
     3001 tgatgtccgg cgtgtcactt accgtggatg acgtggtcgt ggtggtactg gaaatgtcgg
     3061 tcacgaaaaa ttcgtcgtta cctaccttga taaccgcttt ctggttgttg agggtggaaa
     3121 tgcgcgggct tgacagcacc tgcacatcgc cctgcaactg cagcagcgcc agtacggcat
     3181 tgaagtcggt gaaattggca cccaacgtga acacgccgcc aatcagcccc acgttttcgt
     3241 tcaccaggct gctgaaatcc gcctgttcca ggccgcctgc aatcttgttg tcgctggttt
     3301 ccagttcacc gttgtaacc
//
LOCUS       DASUEG010001006         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1870938, whole genome shotgun sequence.
ACCESSION   DASUEG010001006 DASUEG010000000
VERSION     DASUEG010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870938"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFX11_18860"
     CDS             <1..831
                     /locus_tag="VFX11_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940743.1"
                     /translation="PTEDPHARAQLCLSCHQGTQQKLASHDIMGAGHPRLSFELDTFT
                     VLQPEHYVKDDDYRQRKPDISSPQIWATGQISGAERTLELLASKRFHGGGMFPEISFY
                     DCHSCHHPMNDIRWQGGEGIKPGALRLNDAHLQISALLLAELLPAQAQKWRDGMKALQ
                     LAGQQGPDAVKTQAQSLQKQLPDIVARFRQPQTLAQQKALLRRLLSEAQKGAFRDYTS
                     AEQVVMGCSVLFSSLKLDDTLRTTLDNLYQVLDDEHRFQPERLRAAAGKALADVERHL
                     "
     gene            828..1679
                     /locus_tag="VFX11_18865"
     CDS             828..1679
                     /locus_tag="VFX11_18865"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX4940744.1"
                     /translation="MTEHDTGQTAPRAHSSAQTHPGRKRPQNQDSIHMDDGQGLWVLA
                     DGMGGHQGGEIASAIACEEIPAAVADGKSLQDALRVAHRKILARGEQQPALRGLGTTV
                     VVIHQQGHEYELAWSGDSRIYRWRKGRLQQLSEDHSIVARMLRAGLISDEQARTHPHQ
                     HVISSCLGTTSGDGLEIGYRIETWEKGDWLLLCSDGLTDELEDDTIAALLQQHNDLDA
                     ASQALLQAALEAGGRDNVSVALIAAPAAAPEGISQRRPLHPAIWGVLGSVLALVVLLA
                     IKLLRPE"
     gene            1720..2799
                     /locus_tag="VFX11_18870"
     CDS             1720..2799
                     /locus_tag="VFX11_18870"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4940745.1"
                     /translation="MTDDNATRLHIEPIGVTLIAEDGKEFLISESTTVGRSKDAGLMI
                     DDDQISRRHAQLKPLSDGLEVEDLGSSNGTFLNGQRLPKFVARHGDILHFYKHKYSVS
                     IRSQQAPAFDPEATRLHIPEAAPAPVEKPLPAEKPVAVDLPSPAPKAVVSDLAGDKKP
                     ADEKKPAGETKPADADKPERAWWETSDEGPVGTVLLRPGEVDLEAEMTRLFNLGPAQT
                     PRLVGMAGEYSGRIFELGEGKFVIGREAGCAIRLDNDGISVKHAQIIHEGDTWKLVNL
                     LSSNGTFVNSERIQTAYLNSGDELRFGSVALLFQLPGGAIIKSAEAEATAAEPKKSGP
                     ASKTGLIAAIVAFLLVLVVGALVLL"
BASE COUNT          604 a          916 c          889 g          505 t
ORIGIN      
        1 cctaccgaag atcctcacgc ccgcgcgcaa ctgtgcctgt cctgccacca gggtacgcag
       61 cagaaactgg cgagccacga catcatgggc gccggccatc cgcgcctgtc gttcgagctc
      121 gacaccttca ccgtgctgca accggagcac tacgtgaaag acgacgatta ccgtcagcgc
      181 aagccggata tttccagccc gcaaatctgg gccaccggcc agatcagtgg cgcagaacgc
      241 acactggagc tgctggcatc aaagcgattc cacggcggcg gcatgttccc ggaaatcagt
      301 ttttacgatt gccactcctg ccatcatccg atgaacgaca tccgctggca gggaggtgaa
      361 ggcattaagc ccggcgcgct gcggctgaac gacgcccacc tgcagatcag cgctctcctg
      421 ctggcagagc tgctgccggc gcaggcacaa aagtggcgtg acggcatgaa ggcattgcag
      481 ctggccgggc agcagggacc ggacgcagtg aaaacccagg cccagtcgct gcagaaacag
      541 ctgcctgaca ttgttgcgcg tttccgccag ccacagacac tcgcccaaca gaaagccctg
      601 ctgcgccgtc tgctgtccga ggcgcagaaa ggcgccttcc gcgattacac cagtgccgaa
      661 caggtggtga tgggctgcag cgtactgttc agcagcctga aactggacga cactttacgc
      721 accaccctcg acaaccttta tcaggtactg gatgacgaac accggtttca acccgaacgc
      781 ctgcgcgcgg ctgcaggcaa ggcgctggca gatgtggagc gccacctatg actgagcacg
      841 acactgggca gactgccccg cgcgcgcatt cctccgcgca aacccatcct ggccgcaagc
      901 gcccgcagaa ccaggacagt attcacatgg acgacggcca gggcctgtgg gtgctggcgg
      961 atggcatggg cgggcaccag ggcggcgaga tcgccagcgc catcgcctgt gaggaaattc
     1021 ccgccgctgt cgccgacggc aaatcgctgc aggatgccct gcgggtggcc catcgcaaaa
     1081 ttctggcacg cggcgaacag cagccggcct tgcgcggcct tggcaccact gtcgtggtga
     1141 tccaccagca ggggcacgag tacgaactgg cctggagcgg cgacagccgc atttaccgct
     1201 ggcgcaaagg ccggctgcaa cagctcagtg aggatcattc catcgtggcg cggatgctcc
     1261 gggccgggct gatttccgac gagcaggccc gcacccatcc gcaccagcat gtgatcagtt
     1321 cctgtctggg tactacgtct ggcgacgggc tggaaatcgg ttacaggatt gaaacctggg
     1381 aaaaaggcga ctggttgttg ttgtgcagcg acggcctgac cgacgaactg gaagacgaca
     1441 ccatcgccgc cctcctgcaa cagcacaatg atctggacgc cgccagccag gctctgctgc
     1501 aggcagcgct ggaagccggt ggacgcgaca atgtcagcgt tgcgctgatc gcggcgcccg
     1561 cggccgcgcc ggagggcatt tcccagcgcc ggccgctgca cccggccatc tggggtgttc
     1621 tgggttccgt gctggcgctg gtggtgctcc tggccatcaa gctgctgcgg ccggagtgaa
     1681 gtaccatgca ttacgtgatt gactcacagg aaaaccgcaa tgaccgatga caatgccaca
     1741 cgcctgcata tcgaaccgat aggcgtcaca ctcatcgccg aggacggcaa ggaattcctg
     1801 atcagcgaat ccaccaccgt ggggcgcagc aaggacgcgg gattgatgat tgacgacgac
     1861 cagatctccc gccgtcacgc ccagctgaaa ccgctttccg acgggctgga agtggaggat
     1921 ctgggttctt ccaacggcac cttcctgaat ggccagcggt tgccgaaatt tgtcgcccgt
     1981 catggcgaca tcctgcactt ttacaagcac aaatacagcg tcagcatccg gtcgcagcag
     2041 gcacccgcat tcgatcccga ggccacccgt ctgcatatac cggaagcagc ccctgcgccg
     2101 gtggaaaagc ccctcccggc ggaaaagcct gtagccgttg atcttccctc gccagcacca
     2161 aaagcggtgg tgtccgatct ggctggtgac aagaaaccgg ccgacgaaaa aaaaccggcg
     2221 ggcgaaacga agcctgccga tgccgacaaa cccgaacggg cctggtggga aacctcggat
     2281 gaaggcccgg tgggtactgt gctgctccgg cccggtgaag tggacctgga agcagaaatg
     2341 acgcgcctgt tcaatctcgg cccggcgcaa acgccccggc tggtgggcat ggcgggcgaa
     2401 tactccggcc gcattttcga actgggcgaa ggcaaattcg tgatcgggcg tgaagccggc
     2461 tgcgccatcc gcctggacaa cgacggcatc tcggtaaagc acgcgcagat cattcatgaa
     2521 ggtgacacct ggaaactggt gaacctgctg tcgtccaacg gcaccttcgt caacagcgaa
     2581 cggattcaga cagcgtatct gaacagtggc gacgaactgc gtttcggttc cgtggcgttg
     2641 ctgttccagc tgccaggtgg tgcgattatc aaatccgccg aagcggaagc cactgcggcg
     2701 gagccgaaaa aatcaggtcc cgccagcaag acgggcctga ttgctgccat tgtggctttt
     2761 ctgttggtgc tggtggtggg cgcgctggtg ctgctctgac aggccagcgt ccatcaggtg
     2821 atttaccgtt tttttcagcc ttcagttttc acaatccggc gccctggcat ttgccggggc
     2881 tgcttccctt actgtccgtt caattgccag ttgt
//
LOCUS       DASUEG010001007         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1450731, whole genome shotgun sequence.
ACCESSION   DASUEG010001007 DASUEG010000000
VERSION     DASUEG010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450731"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..2872
                     /locus_tag="VFX11_18875"
     CDS             32..2872
                     /locus_tag="VFX11_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940746.1"
                     /translation="MKRRVYPLYVLVTGLLVVCLGVPGGLLVGSQYRARKEHAFADAG
                     AQMRTQLMMTQARVERDLANRNFSSVASEVVMLKLLDDVNLSAVVDELGIVRYSSNFV
                     IVDQPAATQLHQFDGDLFAQMQRNSRSMLVSDESRENLFGYYPLAFSRTENSMRPDRI
                     GALFVHWSLERPYRYLQLQVLREAGYLLLLTLLASGGLFLLLRRKIVRPLRRLQQKAD
                     YAAGGQDVQNFSENGVAETVNLGQALDRMRQQQLTGLQDVRRSEERWLFALDAAGQGV
                     HDWDLVRGQCYFSPQCRRVLGLDATAPLACDDWKKQVLAEDWNFVERRLERHFQGLSD
                     FCNAEYRIMGADGNPHYILMRGRVVERKPDGSPGRFVATHADVTSRRRMEDALRNSEE
                     KYRRLFEMAQEGIWVIDAAGLTTIVNNAMASMLGYKKEEMIGRHLFDFMDEDSRIAAR
                     ENMKRRAQGIEEQHDFEFLASNGRRVFTTMQTAPIFDGNGVYAGAIAGVMDITERKRA
                     EERIRQQALFDELTRLPNRRLLNDRLIQEQARALRHQHVGAVLFIDLDHFKNVNDSLG
                     HPVGDALLIEIAERLQNVVREEDTLARLGGDEFVVLLPELDSNPTTAASQARGVGLKM
                     QEALTPTFDIAGHKLNISCSIGIALYPLDQESIHDILKQADTAMYRAKQEGRSTLCIF
                     NKEMHEAIEQNLRLQMLLPGALQENQFELHYQAQFNHRRELIGAEALLRWQEPTQGFV
                     RPDLFIRAAEESGFIVQLGDWVLRHACAQLRQWQNAGLPVSFQRLAINISARQFAQDD
                     FPQRVLACVQDAGIDPRQIELEITESMLLNRLEQVVEKMRVLHGMGFYIALDDFGTGY
                     SSLSYLRSLPLHKLKIDRAFVRDIQNDRNDRAIVETIIAMARHMELDVIAEGVEDEQQ
                     FDFLREKGCLQYQGYYFARPVPALTFQQQWTP"
BASE COUNT          585 a          853 c          935 g          579 t
ORIGIN      
        1 ctgcaccccg ctggctggaa ggcgtgcgac catgaagcgg cgtgtgtacc ctctgtatgt
       61 actggtgacc ggcctgctgg tggtgtgcct gggcgtgccc ggtgggctgc tggtgggcag
      121 ccagtatcgg gcgcgcaaag agcatgcctt tgcggatgcg ggcgcccaga tgcggaccca
      181 gctgatgatg acgcaggcgc gggtggagcg ggatctggca aatcgcaatt tctccagtgt
      241 tgccagtgaa gtggtcatgc tcaaactgct ggacgacgtc aacctgagtg ccgtcgtgga
      301 tgagttgggt atcgtgcgtt acagctccaa tttcgtcatt gtcgatcagc cggccgctac
      361 ccagttgcac cagttcgacg gtgacctgtt cgcgcagatg cagcgcaact ccagatccat
      421 gctggtcagc gacgaatcac gcgaaaacct gtttggctat tatcctcttg cgttcagccg
      481 gaccgaaaac agcatgcgtc ccgatcgtat cggcgcgctg ttcgtgcact ggagcctgga
      541 gcgcccctat cgctatctgc aactccaggt cttgcgtgaa gcaggctacc tgctgctgct
      601 gacactgttg gcgtccggtg gcctgttcct gttgttgcgt cgcaagatcg tgcggccgtt
      661 gcggcggctg cagcaaaagg ctgactatgc tgcgggtggt caggacgtgc agaacttcag
      721 cgaaaacggc gtcgccgaaa ccgtcaacct gggtcaggcg ctggaccgga tgcgccagca
      781 acagctgacc ggcctgcagg atgtgcgacg cagtgaggag cgctggctgt ttgcgctgga
      841 tgcagcgggc cagggcgttc acgactggga tctggtgcgg ggccaatgct atttttcgcc
      901 gcagtgccgc cgcgtgctgg gacttgacgc caccgcacca ctggcttgcg acgactggaa
      961 aaaacaggtg ctggctgagg actggaattt tgtcgagcgt cgcctggaac ggcattttca
     1021 agggttgtct gatttctgca acgccgagta ccgcataatg ggggccgacg gcaacccgca
     1081 ttacattctg atgcgcggcc gcgtggtcga gcgcaagccc gatggcagtc cgggccggtt
     1141 cgtcgccacc cacgccgacg ttaccagccg ccgccgtatg gaggatgcgc tgcgcaacag
     1201 cgaggaaaaa taccggcgcc tgttcgaaat ggcccaggaa ggcatttggg tgatcgatgc
     1261 tgccggcctc accaccatcg tcaacaatgc gatggccagt atgctgggct acaaaaaaga
     1321 ggagatgatc ggtcgccacc tgttcgattt catggacgag gattcccgca tcgctgcgcg
     1381 ggaaaatatg aaacgcaggg ctcagggcat cgaggaacaa cacgatttcg aatttctcgc
     1441 cagtaacggc cggcgggttt tcaccaccat gcagactgcc cccattttcg atggcaatgg
     1501 tgtgtatgcg ggggccattg ccggcgtcat ggacattacc gaacgcaagc gcgccgagga
     1561 gcgcatccgg cagcaggcgc tgttcgatga actcacccgg ctgccgaacc ggcgcctgct
     1621 caatgaccgg ctgatacagg agcaggcgcg tgcgttgcgc caccagcatg tcggtgcggt
     1681 gttattcatc gatctggatc acttcaagaa cgtcaacgat tccctcggac atcccgtggg
     1741 ggacgccctg ctgatcgaga ttgccgagcg tctgcagaac gtggtgcgcg aagaagatac
     1801 gctggcgcgg ctgggtggcg acgaattcgt ggtgctgctg ccggaactgg acagcaatcc
     1861 caccacggct gcgtcccagg cgcgtggcgt cgggctgaaa atgcaggaag ccctgacccc
     1921 gaccttcgac attgccggcc acaagctcaa catcagctgc agtatcggga ttgcgctgta
     1981 cccgctggac caggaatcca ttcacgacat cctgaaacag gcggataccg cgatgtaccg
     2041 cgccaaacag gaaggccgca gcacgctgtg tattttcaac aaggaaatgc acgaggccat
     2101 cgaacagaac ctgcgcctgc agatgctgct gccaggtgcg ctgcaggaga accagttcga
     2161 actgcattac caggcccagt tcaaccaccg gcgcgaactc attggcgcag aggcgttgct
     2221 gcgctggcag gagccgaccc agggtttcgt gcggcccgat ctgttcatcc gcgcggcgga
     2281 ggaatcgggt ttcatcgtgc agctgggtga ctgggtgttg cgacacgcct gtgcccagtt
     2341 gcggcaatgg cagaacgccg gcctgcccgt cagcttccag cgtctggcca tcaatatcag
     2401 tgcgcgacag tttgcgcagg acgatttccc ccagcgcgta ctggcgtgtg tgcaggacgc
     2461 cggcatcgat ccccgccaga tcgaactgga aatcacggaa agcatgctgc tgaaccggct
     2521 ggagcaggtg gtggaaaaaa tgcgcgtgct gcacgggatg ggtttttaca ttgcgctcga
     2581 cgattttggt accggctatt cgtcgctgtc gtatctgcgc tcactgccgt tgcacaagct
     2641 caagatcgat cgagcctttg tgcgcgacat ccagaatgac cgcaatgatc gcgccatcgt
     2701 cgaaaccatt attgcgatgg cgcgccacat ggaactggat gtgattgccg aaggtgtcga
     2761 ggacgaacag cagttcgatt tcctgcggga aaaaggctgt ctgcagtacc agggttatta
     2821 cttcgcccgt ccggtgccgg cgctcacctt ccagcagcaa tggacgccct aaacgcactc
     2881 cagcagcggt cgtggtgaag gcacgcctgc atcctggcgg aacagggcca ccttcaggtt
     2941 gtcggcgcga ta
//
LOCUS       DASUEG010001008         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_699607, whole genome shotgun sequence.
ACCESSION   DASUEG010001008 DASUEG010000000
VERSION     DASUEG010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699607"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFX11_18880"
     CDS             <1..783
                     /locus_tag="VFX11_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940747.1"
                     /translation="AGELVMPHTRFAACFAAFVRCSLIASVSAFLLSCAALTASAPPT
                     DNASRPLPARKPLQSVVIDEFTHDTQVLRTSDYAFVQAWWIPVEFWQAALSQVNPALA
                     QQSLDVLKPYSMLAVVQADVTALGGFVFYPRDDLQQRLQLQWQPSRGEAVTLHPVSQP
                     NSTLQNLVTQLSPLLSRAMGEMGRNMQFFVFEDVKADGTRVTDPYSAGTLRVNLAPSE
                     TQAAAQLDIELPVNALYEPRLCANGKPAHISWQYCPWDGQPL"
     gene            865..1974
                     /locus_tag="VFX11_18885"
     CDS             865..1974
                     /locus_tag="VFX11_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX4940748.1"
                     /translation="MKTRAAVAWAAGQPLSIEEVDLEGPRAGEVMVKLAATGVCHTDA
                     FTQSGADPEGIFPCILGHEGAGVVVEVGPGVTSVAVGDHVIPLYTPECGKCKFCLSGK
                     TNLCQAIRATQGKGLMPDGTSRFSKNGQPIYHYMGTSTFSEYTVLPEISVAKISKEAP
                     LDKVCLLGCGITTGIGAVLNTAKVQPGSTVAVFGLGGIGLSVIQGAVMAKASRILAID
                     INEDKFEMARMLGATDCVNPKKYDKPIQDVIVDLTDGGVDYSFECIGNVNVMRSALEC
                     CHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGVKGRTQLPDYVNRYMKGE
                     IRIDEFITYTMPLDRINEAFDLMHDGKAIRSVIIY"
     gene            1992..>2373
                     /locus_tag="VFX11_18890"
     CDS             1992..>2373
                     /locus_tag="VFX11_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4940749.1"
                     /translation="MQILEDIRCFDGRQLRVQHASQACACDMIFSVFLPPHAAARKVP
                     VLWWLSGLTCTDQNFVTKAGAQRMAAELGIAIVAPDTSPRGEGVPGDPAASWDFGLGA
                     GFYVDATQAPFDRHYRMYRYVQQEL"
BASE COUNT          447 a          671 c          759 g          496 t
ORIGIN      
        1 gctggggaac ttgtcatgcc gcacacccgt tttgccgcct gttttgctgc atttgtccgt
       61 tgtagtctaa tcgcttccgt ttccgcgttc ctgctcagct gtgctgcgct gactgccagc
      121 gcaccgccca ctgataacgc atcgcgtccg ctgccggcgc gcaaaccgtt gcagtcggtg
      181 gtgatcgatg aattcacgca tgacacccag gtcctgcgta ccagcgacta tgcttttgtg
      241 caagcctggt ggattccggt ggaattctgg caggcggccc tgagccaggt gaatccggca
      301 ctggcgcagc agtcgctcga tgtgctgaaa ccttacagca tgctggcagt ggtgcaggct
      361 gatgttactg cgctgggtgg ctttgtgttt tatccccgcg acgatctgca gcagagactg
      421 cagctgcagt ggcaaccgtc ccggggtgag gcggtgacgc tgcacccggt aagccagccc
      481 aacagcacgt tgcagaatct ggtgacacaa ctgtcgccac tactgagccg cgccatgggc
      541 gaaatggggc gcaacatgca attcttcgtg ttcgaggatg tgaaggccga cggcacccgg
      601 gtgaccgatc cttacagtgc cggcacgctg cgggtaaatc tggcgccgag tgaaacccag
      661 gccgccgcgc agctggatat cgaactgccg gtgaatgcgt tgtatgaacc gcgcctgtgc
      721 gccaacggca agccagccca catttcgtgg cagtattgcc cctgggatgg ccagccactc
      781 tgactggcca accctgtcaa attgcagtag cctcgcaaca gtcgctttcg ctaccactct
      841 cattccaaca acaggatttt tcccatgaaa acacgcgccg ccgtcgcctg ggccgcaggc
      901 cagccattaa gcatcgagga agtggatcta gaagggcccc gcgccggcga ggtgatggtg
      961 aagctggcgg ccaccggtgt gtgccatacc gatgcgttta cgcagagcgg tgctgacccg
     1021 gagggcattt tcccctgcat cctgggccac gaaggcgcgg gcgtcgtggt ggaagtgggg
     1081 ccgggcgtca ccagtgtggc ggtgggcgat catgtgattc cgctgtacac gccggaatgc
     1141 ggcaagtgca aattctgcct gtcgggcaag accaatctgt gtcaggcaat ccgcgccacc
     1201 cagggcaagg gtttgatgcc ggacggtacc tcccgtttca gcaaaaatgg ccaacccatt
     1261 tatcactaca tgggcacctc gacgttttcc gaatataccg tgctgccgga aatttccgtg
     1321 gcgaaaatca gcaaagaggc gccgctggat aaggtctgcc tgctgggttg cggcatcacc
     1381 accggcatcg gcgcggtgct gaacacggcg aaagtgcagc ccggttccac cgtggcggtg
     1441 tttggtctgg gtggaattgg tctgtcggtc atccagggcg cggtgatggc gaaagcgtcg
     1501 cggattctgg cgatcgatat caacgaagac aaattcgaga tggcgcgcat gctgggcgct
     1561 accgattgtg tgaatccgaa aaaatacgac aagccgatcc aggacgtgat tgtggatctc
     1621 accgacggtg gtgtggacta ttccttcgag tgcatcggca acgtgaatgt catgcggtcg
     1681 gcgctggagt gctgtcacaa gggctggggt gaatccatca tcatcggcgt ggcgggcgca
     1741 ggccaggaaa tttccacccg gccgttccag ctggtgaccg ggcgcgtgtg gcgcggcagt
     1801 gcgtttggtg gagtgaaagg ccgcacgcaa ttgccggact atgtgaaccg ttacatgaag
     1861 ggtgagatca ggatcgacga gttcatcacc tacaccatgc cgctggaccg catcaatgaa
     1921 gccttcgatc tgatgcacga tggcaaggcg atccggtcgg tcatcattta ctgattttcg
     1981 ggagcaggcg cgtgcagatt ctggaagata tccgctgttt cgatggccgc cagttgcggg
     2041 tgcagcatgc gtcgcaggcg tgtgcgtgcg acatgatttt ttccgtgttt ctgccgcctc
     2101 atgcggctgc cagaaaagtg ccggtgctgt ggtggttgtc gggtcttacc tgcaccgacc
     2161 agaatttcgt gaccaaggcg ggcgcgcagc ggatggcggc ggagctgggc atcgccatcg
     2221 tcgcgccgga caccagtccc cgtggcgagg gcgtgcccgg tgatccggcg gccagctggg
     2281 attttggcct gggcgcaggt ttttatgtgg atgccacgca agccccgttc gaccgccatt
     2341 accgcatgta ccgctatgtg cagcaggaat tgc
//
LOCUS       DASUEG010001009         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1760010, whole genome shotgun sequence.
ACCESSION   DASUEG010001009 DASUEG010000000
VERSION     DASUEG010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760010"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFX11_18895"
     CDS             <1..234
                     /locus_tag="VFX11_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940750.1"
                     /translation="SFHEATARAFANDRILLDGEIAYAMKLVRCLNRMESLILPKIIA
                     ERALKRYPNLSLPEKMLGGARIYTQVAKNLIRG"
     gene            238..1422
                     /locus_tag="VFX11_18900"
     CDS             238..1422
                     /locus_tag="VFX11_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX4940751.1"
                     /translation="MSKSYYEFFCPVKVVAGSAALEHIPYELSTLSASRPLIITDKGV
                     RGAGLIDHVLAAFAESGMDVIDIYDDVPPDSSLKVVKDITRVYREKQCDSIIAVGGGS
                     VIDTSKGVNILVSENGNDLSDFSGVGVLKKPLKPFFVIPTTAGTGSEVTMVAVISDPD
                     KGVKLPFTSYFLLPNVAILDARMTLTLPPHITAMTAMDAMTHAVEAYTGMAKNPISDA
                     YATSAIRKIAENLPRVLDKPDDADARLELAQASTMAGIAFSNSMVGLVHSLGHSLGAL
                     CHLPHGLCMNLFLPYVLEYNLPQRGDVIGELLLYLAGADVYARTPAHERGRKAIQVIR
                     DMRDEVNRRCKLPRTLSETGKITRDQLPKLAQMALDDGSIIFNPVEVDLDDALAVLNR
                     AW"
     gene            complement(1438..>3763)
                     /locus_tag="VFX11_18905"
     CDS             complement(1438..>3763)
                     /locus_tag="VFX11_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4940752.1"
                     /translation="APIQDSDGKISCIAGVIRDITAARQSNDQIRFLNQLYAALSRTQ
                     RALMDCHDENSLFSRVCQIAVDHGGMAMAWIGTCDAASGKIRPVTSAGRNTDYLDGIS
                     ISSRADLPEGRGPTGTAFREGSAIFIQDFMSSTITSPWHERARRCGWRASAAVGIRRA
                     GKPYAVLTLYHTLPDAFDDKTADLLQEMAANLGHGLDQLDIEAERRQYEQQVQASSRR
                     YHEILETSIDGFWLLDLEGRILSVNSAYVRRSGYSRDQLLTMHVSDLEALHDPAVIAA
                     NIRRLAGEGAVQFESLHRASDGSLWPVEVKAVMLAGEEGRFFAFLRDLSERKRAEEEI
                     INLGFYDPLTGLPNRRLLLERLRHAMAGSSRNQLYCAVLFIDLDHFKVINDTVGHETG
                     DLLLQEVARRLRELVREEDTIARLGGDEFVAVLEELDADINSAMARAGLVADKLLKAF
                     ARPYLLNGEEFICTASIGIVIFRDHDVTIEDILKHSDLAMYGAKKSGRNVVRFFDPEM
                     QRELERRTRLETDLRRSLTEQQLRLYCQAQVDQSGKVIGAEVLLRWQHPERGLVPPLD
                     FIPFAEESGLIIPIGQWLLREACLQLKRWEASAETRPILLSVNVSAREFRQPDFIANM
                     QTILTETGANPARLELEITESLLLEGIDEFIVKMREMKAMGISFALDDFGTGYSSLAY
                     LKKLPLNQLKIDRSFVQDLGRDRSDEAIVQTIIRMSETMGLAVIAEGVESELQRRMLQ
                     QYGCRRFQGYLFGRPQPIEHFADTLINARAASVD"
BASE COUNT          702 a         1130 c         1169 g          762 t
ORIGIN      
        1 tcctttcacg aagccacagc acgggccttc gccaatgacc gcatcctgct ggacggtgaa
       61 attgcctacg ccatgaagct ggtgcggtgt ctgaaccgga tggaaagcct gatcctgccc
      121 aaaatcattg ccgagcgcgc actcaagcgc tatcccaacc tgtcgctgcc ggaaaaaatg
      181 ctgggcggcg cacgcatcta tacacaagtt gccaagaacc tgattcgggg ctgatccatg
      241 tcgaaatcct attacgaatt cttttgtccg gtgaaagtgg tggcgggcag tgccgcgctg
      301 gaacacattc cctatgaact gagcacgctg tcggcctccc gccccctgat cattaccgac
      361 aagggtgtgc gtggcgcggg cctgattgat catgtgctgg cagcttttgc tgaaagcggc
      421 atggatgtga tcgacattta tgatgacgtg ccgccggatt cttcgctgaa agtggtgaag
      481 gacatcaccc gcgtctaccg cgaaaaacaa tgcgattcca tcatcgccgt cggtggtggc
      541 tcggtcatcg acaccagcaa gggcgtgaac atcctggtat cggaaaacgg caatgatctg
      601 tcggatttct cgggtgtggg cgtgctgaaa aagccgctga agcctttctt cgtgattccc
      661 accaccgccg gcaccggttc tgaagtgacg atggtggcgg tgatttccga tccggacaag
      721 ggtgtgaagc tgccattcac gtcctatttt ctgctgccga acgtagcgat tctcgacgcg
      781 cgcatgacac tcacactgcc gccgcacatt acggcaatga cggcaatgga tgccatgacc
      841 cacgctgtcg aggcctatac cggcatggcg aaaaatccca tcagcgatgc ttacgccacg
      901 tccgccattc gcaagatcgc ggaaaacctg ccccgcgtgc tggacaaacc cgacgacgca
      961 gacgcccggc tggaactggc ccaggcgtcc accatggccg gcattgcatt ttccaattcc
     1021 atggtgggac tggtgcattc actcggccat tccctgggcg ccctctgcca tttgccccat
     1081 ggcctttgca tgaacctgtt cctgccctat gtactggaat acaacctgcc gcagcgggga
     1141 gacgtgatcg gtgaactgct gctgtatctg gcgggtgccg atgtctatgc gcggaccccg
     1201 gcacacgagc gcggccggaa agcgatacag gtgatccgcg acatgcgcga tgaggtcaac
     1261 cgtcgctgca agctgccgcg cacgctgtcg gaaaccggca agatcacccg cgaccagctg
     1321 ccgaagctgg cacagatggc gctggatgat ggctccatca ttttcaatcc ggtggaagtg
     1381 gatctggatg acgcgctggc ggtgttgaac cgcgcctggt aagcgaatcc cgcgccgtca
     1441 gtcaacgctg gcggcgcggg cgtttatcaa cgtgtcagca aagtgttcga tcggctgcgg
     1501 tcggccaaac aggtagccct ggaagcgccg gcagccatat tgctgcagca tccgccgttg
     1561 cagttcggat tccacccctt ccgcaattac cgccagtccc attgtttcgc tcatgcggat
     1621 gatggtttgc acgatggcct cgtcggagcg gtcccgcccc aggtcctgca cgaagctgcg
     1681 atcgatcttg agctggttca gcggcagttt tttcagatag gccagcgacg agtagccggt
     1741 gccgaaatcg tccagcgcga acgagatgcc catcgctttc atctcgcgca ttttcactat
     1801 gaattcatcg atgccttcca gcagcaggct ttcggtgatt tccagttcca gccgcgccgg
     1861 attggcgccg gtctccgtca gaatcgtctg catgttggca atgaaatccg gttgccggaa
     1921 ttcccgtgcg ctgacattca ccgacaacag tattggccgc gtttccgcac tggcttccca
     1981 ccgtttgagc tgaagacagg cttcccttag cagccactgg ccgatgggaa tgatcagccc
     2041 gctttcttcg gcgaagggaa tgaaatccag ggggggtacc aggccgcgct cgggatgctg
     2101 ccagcgcagc agaacttcgg caccgatcac cttgccggac tggtccacct gggcctggca
     2161 gtacaggcgc aattgctgct cggtcaggct gcggcgcaga tccgtctcca gccgggtgcg
     2221 ccgctccagt tcgcgctgca tttccggatc gaaaaaacgc accacgttgc gcccgctttt
     2281 cttggcgccg tacatggcca gatccgaatg cttgaggatg tcttcgatgg taacgtcgtg
     2341 atcgcggaaa atcacgatgc cgatgctggc ggtgcagatg aattcctcgc cattgagcag
     2401 ataggggcgg gcgaaggcct tcagcaattt gtccgccacc agtccggcac gggccatggc
     2461 gctgttaatg tcggcgtcca gttcttccag cacggccacg aactcgtcgc cgcccagccg
     2521 ggcgatggta tcttcctccc gcaccagttc gcgcaggcgt cgtgctactt cctgcagcag
     2581 cagatcgccc gtttcatggc ccacggtgtc gttgatcacc ttgaaatgat ccagatcgat
     2641 gaacaggacg gcgcagtaca actggttgcg gctgctgccc gccatggcgt ggcgcaggcg
     2701 ctccagcagc agccggcggt tgggcaggcc ggtgagggga tcgtagaaac ccaggttgat
     2761 gatctcttcc tcggcgcgct tgcgctcgct caggtcgcgc aggaatgcga aaaaccggcc
     2821 ctcttcccct gccagcatca cggccttgac ttccaccggc cagagcgagc cgtcactggc
     2881 gcggtgcagg gattcgaact ggacggcgcc ttcgccggcc agacggcgga tattggcggc
     2941 gatgacggcg ggatcgtgca atgcttccag atcgctgacg tgcatggtca gcagctggtc
     3001 gcgggaatag ccggagcggc gcacataggc actgttgacg ctgagaatgc ggccttcgag
     3061 atcgaggagc cagaagccgt cgatggaggt ttccagaatc tcgtgatagc ggcgcgagct
     3121 ggcctgcacc tgttgttcgt actggcggcg ctcggcctca atgtccagct gatcgagccc
     3181 gtgtcccaga ttggcggcca tttcctgcag caggtccgcg gtcttgtcgt cgaaggcatc
     3241 gggcaaggtg tggtacaggg tgagcaccgc atatggcttg ccggcgcggc ggattccaac
     3301 ggcggcggag gcgcgccagc cacagcggcg cgcccgctcg tgccacggtg aggtaatggt
     3361 ggaggacatg aaatcctgaa tgaagatggc gctgccctcg cggaatgcgg tgccggtggg
     3421 gccgcgacct tccggcaggt ccgcccgcga tgagatgctg atgccatcga ggtagtcggt
     3481 gttgcgtccg gcgctggtca ccggccggat ttttccgctg gcggcatcgc aggtgccgat
     3541 ccaggccatg gccatgccac cgtggtccac ggcgatctgg cagacccggc tgaacaggct
     3601 gttttcgtcg tgacagtcca tcagcgcgcg ctgggtgcgg gacagggcgg catacagctg
     3661 gttgagaaag cggatctggt cgttgctctg gcgggcggcg gtgatgtcac ggatcacgcc
     3721 ggcaatgcag gagattttcc cgtcgctgtc ctggatcggg gcg
//
LOCUS       DASUEG010001010         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_676938, whole genome shotgun sequence.
ACCESSION   DASUEG010001010 DASUEG010000000
VERSION     DASUEG010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676938"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..623)
                     /locus_tag="VFX11_18910"
     CDS             complement(39..623)
                     /locus_tag="VFX11_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940753.1"
                     /translation="MPTFRHFICLCASILVFTGCDTTSKPDSMPRELRQSSVVSTSTP
                     DFAPAPGATLAWKSDLRVKLSPGVHNDPAMVAFIKAEVDRQLQAKGYGFAPAGTTPTF
                     QLEAVLIMGDELNETQLREALGFDPGLVGKDTTYEKGSMLLFLIDPASLQTEWLAVVQ
                     VFAAPDLPQDVRQERARFGIGQLMSALPSVNGAQ"
     gene            691..1332
                     /locus_tag="VFX11_18915"
     CDS             691..1332
                     /locus_tag="VFX11_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4940754.1"
                     /translation="MQYRIVPVTQFEQNCSLIWCEQTRKAAVVDPGGYLERILTAVKQ
                     EGVTLEKILLTHAHIDHAGATADLAEREQLPIIGPHKGDEFWIQGLPQQGAMFGFKPS
                     RVFTPDRWLEDGENVQVGNVTLSVLHCPGHTPGHVVFFDPTSKLAIVGDVLFNGSIGR
                     TDFPGGNHQQLIDSIKQKLFPLGDDIDFIPGHGPMSTFGNEKKFNPFVGGKWG"
     gene            complement(1362..2144)
                     /locus_tag="VFX11_18920"
     CDS             complement(1362..2144)
                     /locus_tag="VFX11_18920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4940755.1"
                     /translation="MSACSGFFFYPMKQMVRTPADVGLAYEDLAFTTRDQVQLHGWLL
                     HAEKPRGLVLFLHGNAENISTHLGSVYWLPEQGYEVLLLDYRGYGRSEGEPDFPEVYA
                     DVDAAYRWAQQYAAQKQLPLFILGQSLGAAISSYYFGTLPRADVSYKAVALDAVFSGH
                     RDIARDVAGRSIITWPFQFIVPYLVPKDYDPKAHIAGFAPTPLLFFHSPEDQVIPYQQ
                     GEVVFRHASEPKAWVTTAGPHIATFNYQSNRDRLLQFFDAHR"
     gene            complement(2218..>3616)
                     /locus_tag="VFX11_18925"
     CDS             complement(2218..>3616)
                     /locus_tag="VFX11_18925"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX4940756.1"
                     /translation="DPEHVESGSTWLSYAVNFGAEYHPEDNSILYAWKGLLGGYPGFF
                     SVLPYHEKISEYNRIENRDLWEYRLNLTPDETHLLVWHLWELRNIDFDYFFFDENCSF
                     RLLELLQLARPGVDLTSGFEVRAIPVDTVRRVQDAGLVRQVEYRPAAATDLQFQADQL
                     TDHQQALAWDLAHRQRPLDDPQLTALPQEQQAQILRVAYKHLRYEQGKAARDDALAQH
                     SLALLRALKNQATDATATPPAPVTPESGHDTLLVGIAGGRSDGRGLLDLSLRASYHDL
                     ADNPSGYLDGAAINLGEMQLRQREDDSLQIELLNFVDIRSHAPRSRFFSPITWRVQAG
                     FDRVYAEDEQGETDDALTARVNGGAGITLPLGDRHLVYGMGMARLEYDKLLEENLATG
                     AGALAGALFYLPIGTLQLESDYYRFSDGHERSDTRLIHNLPLGKNDAIRLNALHRKQV
                     ETRFDEFSLEYRHYF"
BASE COUNT          700 a         1032 c         1164 g          720 t
ORIGIN      
        1 tcgggcatgg cgctatccct gcgcggctgg gctgtcactt actgcgcacc gtttacggac
       61 ggcagcgcgc tcatcaactg gccgatgccg aagcgggcac gttcctggcg tacgtcctgg
      121 ggcagatccg gcgcggcaaa aacctgcacc acggcaagcc attcggtttg caggctggca
      181 ggatcgatca ggaacagcag catgctgcct ttttcgtagg tggtgtcctt gccgaccaga
      241 cccgggtcga agcccagcgc ttcgcgcaac tgggtttcat tcagttcatc gcccatgatc
      301 agtacggctt ccagctgaaa agtgggtgtg gtgcctgccg gcgcgaaacc gtagcccttg
      361 gcctgcaact ggcggtccac ttccgccttg atgaaggcca ccatggcggg atcgttgtgg
      421 acacccggcg acagcttcac gcgaaggtcg cttttccagg ccagcgtggc gcccggtgcg
      481 ggtgcgaaat ccggcgtgct ggtggacacg accgaggatt gccgcagttc ccgtggcatg
      541 ctgtccggct ttgaagtggt gtcgcagccg gtgaatacca gaatgctggc gcacagacag
      601 ataaagtgac ggaaggtggg catgagtcgc atccatggag ggcaagcaaa gctgatatca
      661 tagcagacaa atttgccgga ggaattcgcc atgcaatacc gcattgtccc tgtgacgcag
      721 ttcgaacaga actgttccct gatctggtgt gaacagaccc gcaaggccgc cgtggtggac
      781 ccgggcggct atctggaacg tattctgacc gccgtaaagc aggagggcgt gacgctggaa
      841 aaaatcctgc tgacccacgc gcacatcgat cacgccggtg ccaccgccga tctggcggag
      901 cgcgagcagc tgccgatcat cggtccgcac aagggtgatg aattctggat tcagggtctg
      961 ccgcagcagg gggcgatgtt tggattcaaa ccgtcgcggg tattcacgcc ggaccgctgg
     1021 ctggaggacg gtgaaaatgt gcaggtgggc aatgttacgc tcagtgtttt gcactgcccg
     1081 ggccacacgc cggggcacgt ggtgtttttc gatccgacct caaaactggc gattgtcggc
     1141 gacgtgctgt tcaacggttc catcggccgc acggattttc ctggcggcaa tcatcagcag
     1201 ctgatcgatt ccatcaagca gaaactgttt ccgctgggcg acgatatcga tttcattccc
     1261 ggccacggtc ccatgtcgac gttcggcaac gagaaaaaat tcaatccgtt cgtgggcggg
     1321 aagtggggct gagggcgctg tcccgggttt ggcgatgtga ttcagcgatg ggcgtcaaaa
     1381 aactgcagca gccgatcgcg attggactgg tagttgaagg tggcgatgtg cggccctgcg
     1441 gtggtgaccc aggctttggg ttcgctggcg tgcctgaaca ccacttcgcc ctgttgatag
     1501 ggaatcacct gatcctcggg gctgtgaaaa aacagcagcg gcgtgggcgc gaagccggcg
     1561 atgtgggctt tcggatcgta atccttcggc accagatagg gcacgatgaa ctggaacggc
     1621 caggtgatga tgctgcggcc ggcgacgtca cgggcgatgt cgcggtggcc cgagaaaacg
     1681 gcgtccagcg ccacggcctt gtagctgaca tcggcgcggg gcagggtgcc gaaataataa
     1741 ctgctgatgg cagcgcccag gctctggccc aggatgaaca gcggcagttg tttttgagcc
     1801 gcgtactgtt gtgcccagcg ataggcggca tcgacgtcgg catacacttc gggaaaatcc
     1861 ggttcccctt ccgagcggcc gtagccgcga taatcaagca atagtacttc atagccctgc
     1921 tccggcagcc agtatacgct gccgaggtgg gtactgatgt tttccgcgtt gccgtgcagg
     1981 aacagcacca gaccccgggg cttttcagcg tgcagcagcc agccgtgcag ctggacctga
     2041 tcgcgcgtgg tgaaagcgag atcctcatag gccagaccga catctgccgg tgtgcgcacc
     2101 atctgtttca tcggatagaa aaagaagccg ctgcaggcgc tcatggtggt taccactgtt
     2161 agcagaaagc acaggcgtac aaggacgcgg ataggcaagc gtcgcaccgg caagcgatca
     2221 aaaatagtgg cgatattcca gactgaattc gtcaaagcgg gtttccactt gtttgcggtg
     2281 cagggcgttc aggcggatgg cgtcattctt gcccagcggc aggttgtgaa tcaagcgggt
     2341 gtcggaacgt tcgtggccat cgctgaaacg gtagtagtca gattcgagtt gcagggtgcc
     2401 gatcggcaga tagaacaatg cgccggccag cgcgccggca ccggtcgcca gattttcttc
     2461 cagcagcttg tcatattcca gccgcgccat acccatgccg tacaccagat gacgatcgcc
     2521 cagcggcagc gtgatgccgg cgccgccgtt gacacgggca gtcagggcgt cgtcggtttc
     2581 gccctgctcg tcctcggcgt agacgcggtc gaagccggcc tgcacccgcc aggtgatggg
     2641 ggaaaagaag cggctgcgcg gtgcgtggga tcggatgtcg acaaaattca gcagttcaat
     2701 ctgcagcgaa tcgtcttcgc gctgacgcag ctgcatttcg ccgagattga tggcggcacc
     2761 gtcgagatag cccgagggat tgtctgccag atcgtggtag ctggcgcgca ggctcaggtc
     2821 cagcaggccc ctgccgtcgc tgcgaccgcc ggcaataccg accagcaggg tgtcatgacc
     2881 ggattcaggc gtgacgggtg ccggtggtgt ggctgtcgca tcggttgcct gattcttcag
     2941 cgcacgcagc agcgccaggc tgtgttgcgc cagtgcgtca tcccgtgcgg ccttgccttg
     3001 ttcgtagcgc aggtgtttgt acgccacccg caggatttgt gcctgttgct cctgcggtaa
     3061 tgccgtcagt tggggatcgt ccagagggcg ttggcggtgc gccagatccc aggccagcgc
     3121 ctgctggtgg tccgtcagct ggtctgcctg gaattgcaga tcggtggccg cagcaggacg
     3181 atactccacc tgacgcacca gccccgcatc ctgcacccgg cgcaccgtgt ccaccggaat
     3241 cgcccgcacc tcgaagccac tggtgaggtc cactccaggc cgtgccagct gcagcagttc
     3301 cagcagccgg aaagagcagt tttcgtcaaa gaagaagtaa tcgaaatcga tattgcgcag
     3361 ttcccacagg tgccacacca gcaggtgggt ttcgtccggc gtcaggttga ggcggtattc
     3421 ccacaggtcg cgattttcga tgcggttgta ctcgctgatt ttctcgtggt acggcagcac
     3481 gctgaaaaat ccggggtaac cgcccagcag tcctttccag gcgtacagga tcgaattgtc
     3541 ctccggatgg tattcggcac cgaaattgac cgcgtagctg agccaggtgg aaccgctttc
     3601 cacatgctcc gggtcg
//
LOCUS       DASUEG010001011         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1031084, whole genome shotgun sequence.
ACCESSION   DASUEG010001011 DASUEG010000000
VERSION     DASUEG010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031084"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFX11_18930"
     CDS             <1..1065
                     /locus_tag="VFX11_18930"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX4940757.1"
                     /translation="LPGNSALFDFSGPVPYDNAPRLERRDFVQNSNDSYWLTNPAQPI
                     SGPSILYGGENKAQTLRSRMGQKMLQDAAGDDGKFSASELEAALFSQRSYLAENVLED
                     LLVQCQSQGDVPVLIKNAAGETSSVNIAGTCAALAQWDGHFHRDSRGAHAFREFAEQF
                     AKNPQWAVPFDASQPTTTPNTLLTNEFTLQQLAVATLNLQAADIAPDAELGDVQFIER
                     ANPDGSPGNERLPFAGSNNIEGGFNVFRPVYSLDGSTLPRHLYKSLPGTQLSAEAQGY
                     PLDYGSSWMMVVEFTRKGPKARGLMTYSQSVDTTSPHALDQSRLYSEQPRLQPIPFRE
                     RDIAAHTQSELRLRLDIRAN"
     gene            1178..2917
                     /locus_tag="VFX11_18935"
     CDS             1178..2917
                     /locus_tag="VFX11_18935"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX4940758.1"
                     /translation="MTTAKRSLRALLQPLALVCATGLLTGCPHDIYLKTGLGVLRGTL
                     EDNGKVVSWKGVPYAQAPVGDLRWQAPRPAQPWHGVRDARDFGSACTQIGSMYGPPAQ
                     GSVVDINLRDVFDKPVGSEDCLFLNLWRPASKKKNLPVVLFIHGGSHMVGTSSYYRGD
                     RLAQEDMIFISVNYRLNLFGWLNHPALKVADDGSDPLAPLTNSGNFATLDLIQALQFV
                     RNHIDEFGGDPDNVTIMGQSAGASSVFSLLVSPLAQGLFDKAVMLSPGLINQSPETSF
                     NYANGLLVALALQAGLATDADSAAVWLATQTPQQLRAFLYSRTPQQLVTATQLDPRLS
                     LTPAILRDGLVQPADPNAAVASGQFNNVPVLVGLTQEEGKLFTRPAFLITSQERWTLM
                     QTFNPDDPASTPTTLEQIVRPELLPADRPQQGTCGAEDFIVGGYNAYANLCGSPFPTA
                     LFRQFQDQALLPLLAMHQPALYAYHWGWNQQPEPWRTVYGAAHGSDVPFLLGLFDTPL
                     FAHGYTQQNAPGRQALSDHALQALAAFAKTGDPNHADLPVAWLPWSPAPGAPKRLLLD
                     ADDEHALLDMSAQ"
     gene            complement(2967..>3871)
                     /locus_tag="VFX11_18940"
     CDS             complement(2967..>3871)
                     /locus_tag="VFX11_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4940759.1"
                     /translation="AGLLTEKGPGSELFDEILADSVKQFQREQYLDVDGAVGKSTRAV
                     LNVPVQVRIDQVRANLERGRWLLHEIKGDFVLVDIAGFSIHFYRNGQPVWSSRVQVGR
                     PFRSTPIFKSRVTYFTFNPTWTIPPTIFKEDVLPKVRKDPDYLAKNQWQALDSAGKPL
                     DPATIDWNRPGNILLRQAAGPANPLGQVAIRFPNPYAVYLHDTPSKSQFGKGMRAFSS
                     GCIRVERPLELVELLFNDPQKWSREQIEAAIATGATRDVGLVTPVPILLAYYTVAVGG
                     DGRILFKHDIYDRDREVLAALDRR"
BASE COUNT          740 a         1342 c         1118 g          671 t
ORIGIN      
        1 ctgccaggca attccgcgct gttcgatttc agcggaccgg tgccctatga caatgcgccc
       61 cggctggagc gccgcgattt cgtgcagaac tccaacgaca gctactggct gaccaatccg
      121 gcgcaaccca tcagcggtcc gtcgattctg tatggcggtg aaaacaaggc gcagacgttg
      181 cgttcgcgca tgggccagaa aatgctgcag gatgctgccg gtgacgacgg caaattctct
      241 gcgtcagaac tggaagccgc gctgttcagc cagcgcagtt atctggcgga aaatgtgctg
      301 gaggatttgc tggtgcaatg ccagagtcag ggcgatgtgc cggtgctgat aaaaaatgcc
      361 gccggcgaaa catccagtgt gaatatcgcc ggcacctgcg cggcactggc gcagtgggac
      421 ggccacttcc accgtgacag ccgcggtgcc cacgccttcc gcgaattcgc cgagcagttt
      481 gcgaaaaatc cgcagtgggc cgtgccgttc gatgcgagcc aacccaccac aacgccaaac
      541 accctgctga ccaacgaatt caccctgcaa caactggcgg tagccacact caatttgcag
      601 gcagcggata ttgcaccgga tgcagaactg ggcgacgtgc agttcatcga gcgcgccaat
      661 cccgatggca gccccggcaa cgaacgcctg ccctttgccg gcagcaacaa tatcgagggc
      721 ggcttcaacg tcttccgtcc cgtgtactcg ctggacggct ccaccctgcc acgccatctc
      781 tacaaatcgc tgcccggcac ccagctcagc gccgaagcgc agggctaccc gctggattac
      841 ggctccagct ggatgatggt ggtggaattc acccgcaagg gcccgaaagc gcgggggctg
      901 atgacctatt cgcaatcggt cgacaccacc tcgccgcacg cactggacca gtcgcgtctt
      961 tattcggaac agccacgcct gcaaccgatt ccgttccgcg agcgtgatat tgccgcccac
     1021 acgcaatcgg aactgcggct gcgcctggac atccgcgcaa actgattcct gcttttttta
     1081 acctgcggca agcgctggca cccgtcacgc ctgccgcact tttattccat caaaacggtg
     1141 gtccatctaa aaacgaaaac gcttgagagg gaacaacatg accacagcaa aacgttcgct
     1201 gcgcgccttg ctgcagccac tggcactggt gtgcgcaacc gggctgctca ccggctgccc
     1261 ccatgacatt tatctgaaaa ccggtctggg tgtgctgagg ggcacgctgg aagacaacgg
     1321 caaagtggtg agctggaaag gcgtgcccta tgcccaggca ccggtgggcg atctgcgctg
     1381 gcaggcgcca cgcccggcac aaccctggca cggcgtacgc gatgcccgcg actttggcag
     1441 cgcctgcacc cagatcggca gcatgtacgg tccgccggcc cagggcagcg tcgtggacat
     1501 caacctgcgc gacgttttcg acaagccggt gggcagcgag gactgcctgt tcctcaacct
     1561 ctggcgcccc gcgtcgaaaa agaaaaacct gccggtggtg ttgttcatcc acggcggcag
     1621 ccacatggtg ggcaccagtt cctactaccg cggcgaccgg ctggcgcagg aagacatgat
     1681 tttcatctcg gtgaactatc gtctgaacct gttcggctgg ctcaaccatc cggcgctgaa
     1741 agtggcagac gatggcagcg atccgctggc gccgctcacc aactccggca acttcgccac
     1801 cctggatctg atccaggcgc tgcagtttgt gcggaaccac atcgatgaat tcggcggtga
     1861 tcccgacaac gtcaccatca tgggccagtc cgccggtgcc tccagcgtgt tcagcctgct
     1921 ggtgtcgccg ctggcgcagg gcctgttcga caaggccgtg atgctgagcc cgggcctgat
     1981 caaccagtcc ccggaaacca gtttcaatta cgccaacggc ctgctggtgg cgctggcgct
     2041 gcaagccggc ctggccaccg acgccgacag tgccgccgtc tggctggcca cccagacccc
     2101 gcagcagctg cgcgcattcc tctacagccg caccccgcag cagctggtca ccgccaccca
     2161 actcgatccc cgcctcagcc tcacgcccgc gatcctgcgc gacggtctgg tgcaacccgc
     2221 cgatcccaat gccgccgtgg ccagcggcca gttcaacaac gtgccggtac tggtgggcct
     2281 gacgcaggag gaaggcaaac tcttcacccg ccccgccttc ctgatcacca gccaggaacg
     2341 ctggaccctg atgcagacct tcaatccgga tgatccggcc tcaacaccca cgacgctgga
     2401 acagatcgtg cgcccggaac tgttgccggc ggatcgcccc cagcagggca cctgcggcgc
     2461 cgaagacttt atcgtcggcg gctacaacgc ctacgccaat ctgtgcggct cacctttccc
     2521 caccgccctg ttccggcagt tccaggacca ggcactgctg cccctgctgg ccatgcacca
     2581 gcccgccctg tacgcctacc actggggctg gaaccagcag ccggagccct ggcgcaccgt
     2641 ctatggcgcc gcccacggca gcgacgtgcc cttcctgctg ggcctgttcg acaccccgct
     2701 gtttgcccat ggctacaccc agcagaacgc gccggggcgg caggcactgt ccgaccatgc
     2761 gctgcaggcg ctggcagcat tcgccaaaac cggcgatccc aaccatgcgg acctgcccgt
     2821 cgcctggctg ccctggtcgc cggcaccggg cgcccccaaa cgcctgctgc tggatgcgga
     2881 cgatgagcac gccctgctgg acatgtcggc gcaataaaaa aaggggaagc ccatcgcttc
     2941 cccttttttc gtcggaaccg gttgtgtcag cgccggtcca gcgccgccaa aacctcacga
     3001 tcccgatcgt agatgtcatg cttgaacagg atgcggccgt caccgcccac cgccaccgtg
     3061 taataggcca gcaggatcgg caccggcgtc accaggccca catccctggt agcgccggtg
     3121 gcaatggccg cctcgatctg ctcccggctc cacttctgcg gatcgttgaa cagcagctcc
     3181 accagttcca gcggccgctc cacccggatg cagccggaac tgaacgcccg catgcccttg
     3241 ccaaactggc tcttgctggg ggtatcgtgc agatagaccg cgtaaggatt ggggaagcgg
     3301 atcgccacct ggcccagcgg attggccggc cccgccgcct gccgcagcag gatattgccc
     3361 ggccggttcc agtcgatggt cgccgggtcc agcggcttgc cggcactgtc cagcgcctgc
     3421 cactggttct tcgccaggta gtccgggtcc ttgcgcacct tgggcagcac atcttccttg
     3481 aagatggtgg gcggaatggt ccaggtggga ttgaacgtga aataggtcac ccgcgacttg
     3541 aagatgggcg tgctgcggaa gggcctgcct acctgcaccc gtgaactcca caccggctga
     3601 ccattgcggt aaaagtggat gctgaaacct gcaatgtcca ccagcacgaa atcgcccttg
     3661 atctcgtgca gcagccagcg gccccgctcc agattggccc gcacctgatc gatgcgaacc
     3721 tgcaccggta cattcagtac cgcccgggtg ctcttgccca cggcaccgtc cacgtccaga
     3781 tactgttcac gctggaactg ctttaccgag tctgccagga tctcatcaaa cagttcgctg
     3841 ccaggaccct tctccgtcag caggcccgcc g
//
LOCUS       DASUEG010001012         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2003173, whole genome shotgun sequence.
ACCESSION   DASUEG010001012 DASUEG010000000
VERSION     DASUEG010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003173"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFX11_18945"
     CDS             complement(<1..186)
                     /locus_tag="VFX11_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940760.1"
                     /translation="MTFITPKQFLEQRLTMIETELDRLGVPHLLPLVQRITRNFCNFP
                     FDLDMEPAQQLLGKLSEL"
     gene            581..979
                     /locus_tag="VFX11_18950"
     CDS             581..979
                     /locus_tag="VFX11_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940761.1"
                     /translation="MGALGTTARAETQGYAVINPTATADQRDLLAVVVSLKFPPNVTT
                     VGQALTALLERSGYRLAYGDNTDPQLSTLMHLPLPQVHRSLGPIRLRDALQALAGSAW
                     ILVEDPVNRLVSFDLDSRFRLAAHGAGEGV"
     gene            976..1971
                     /locus_tag="VFX11_18955"
     CDS             976..1971
                     /locus_tag="VFX11_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940762.1"
                     /translation="MSRTTNNLRVVDDREIVIDPAAGDETFDPEIDLGAIRPDDDIEE
                     SPQTATAPKRGGGLSGMLRTVLIGFGVLLILLVAMVMYLRGSRGPSTEPPVTASVPVL
                     ESALSGVGRVERGEVFEAAIEVEKEVASRPQEQSVEPHVTVPVAPADATPDAALPTDT
                     ITPNDTVKSVIAEMQQVLAEHRQLIQDQLEQNKQFQAELQAIRTSSEATSLRVDSVVA
                     DLAQVRHSLEDLTARETKKVATLQRQLAAKNEQARQQARHAEAQRSEPPPFRVASVTL
                     WGSDYLATVTLLSGQQRDVTVGELLDGWKIDAISATGVSVTRLRDGAKLNLAAGV"
     gene            2365..>2568
                     /locus_tag="VFX11_18960"
     CDS             2365..>2568
                     /locus_tag="VFX11_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940763.1"
                     /translation="MNAAPWDSLTPEQAAGAWQLTTAEWNQYRKLMQGPARYYATEMP
                     PLMVLAMYADTDHELAHYAELLAQ"
BASE COUNT          511 a          690 c          776 g          591 t
ORIGIN      
        1 gagttcgctc agcttcccca gcagttgctg cgctggttcc atgtccagat cgaacgggaa
       61 attgcagaaa ttgcgcgtga tgcgctgcac cagcggcaac agatgtggca cacccaggcg
      121 atcgagctcg gtttcaatca tggtcagacg ctgctccaga aattgttttg gggtgatgaa
      181 ggtcacagta gcgcttcctt ttgggtgctg tcgtttgaat tgattcagat ggcttcggag
      241 tcatttttta ggcaggcgcc gccttttgcc attttcgcct tttgtgtaaa cccttaccct
      301 ttatgcagtt taaccccttg catatttagc cgttaatcct ttccgtgccg cccgcccatt
      361 caagggcggc acccttttgg gtaggcgccc ttacccctga ggccctttcc gcctttttgc
      421 taaaaccggc ccgtctggtg ctggttaaat aaagcgcacg aagccatctg aatcaattcg
      481 cacgacagag tgttgaccct tcggtacggt gcctccacac aacggaggtt cagccatgca
      541 tgtgattcga tcccttttaa tttgcaccag tctgcttgtc ttgggtgccc ttggcacaac
      601 ggcccgtgcc gaaacccagg gctatgccgt catcaacccg acggcgaccg ctgaccagcg
      661 tgatctgctg gcagtagtgg tgagcctgaa atttccaccc aacgtcacca ccgtgggaca
      721 ggcgttgaca gcactgctgg aacgctccgg ttatcgcctc gcctatggcg acaataccga
      781 tcctcagcta tcgaccctga tgcacttacc cctgccgcag gtacaccgat cattagggcc
      841 aatacgcttg cgggatgctc tgcaggcact ggcaggcagc gcctggattt tggtggaaga
      901 cccggtgaat cggttggtga gttttgatct ggattcccgt tttcgcttgg ctgctcatgg
      961 agccggggag ggtgtatgag ccgtacaaca aataatctgc gagtggtaga tgacagggaa
     1021 attgtgatcg atccagcggc gggagacgaa acatttgatc ctgagatcga cttaggtgca
     1081 atccggcctg atgacgacat tgaggaaagt ccgcagacgg ccaccgctcc caagcggggt
     1141 ggtggtctgt ccgggatgtt gcgcactgtt ctgattgggt ttggggtctt attgattctg
     1201 ctggtggcca tggtgatgta tttgcgtggc agcagaggcc catcaacgga accgcctgtg
     1261 accgccagcg tgccggtgtt ggaaagtgcg ctgtctgggg tcgggcgggt tgagcgtggc
     1321 gaagtatttg aagcagctat tgaggtggaa aaggaggtgg cgagcaggcc acaggaacag
     1381 tccgtagagc cccacgtgac tgttcctgtg gcaccagctg acgccacgcc cgacgctgct
     1441 ctgccaacag ataccatcac gcccaacgac acggtgaaat ctgtaatagc ggaaatgcag
     1501 caggttcttg ctgaacatcg gcaattgatc caagaccagc tggagcagaa caagcaattc
     1561 caggcggagc ttcaggccat ccgaacttcc agtgaggcga cgagcttgcg agttgattca
     1621 gttgtggcgg atctcgctca agtgcgtcac tcgctggaag acctcactgc ccgcgaaacg
     1681 aagaaggtag cgacgcttca gcgtcagttg gcagcgaaga acgaacaagc caggcagcag
     1741 gcccgtcacg ctgaggcgca gcgcagcgag ccaccaccgt tccgtgtggc gtcggtgacg
     1801 ctgtggggtt ctgattatct cgccacggtg acattgctct ctggccagca acgggacgtg
     1861 acggttgggg agttgctcga cggctggaaa atcgatgcca tttccgcgac cggcgtatct
     1921 gttacgcgct tgcgcgatgg cgccaagttg aatttggcag ctggagtgtg acgatgcgac
     1981 tcgcctttca ctggtcatcg aaaacagttt tggttggctg cattttgttg gcgggttgcg
     2041 cgacgccccg tagttctgcc gttacggttc agcctgttgt gtgcacggtt cactaccgcg
     2101 atctaactca gcgttgggtc gcggcggatt atcgttgtgc ggagaccagc cttccgctgc
     2161 cgctgccgcg tatccgcaca ttggtggaca gacatgcctc cgcgcagggg ccgatagcgg
     2221 gaggaataca tgagatgcgt tgacgttcta tttcggtgga tggcctgcgc aggattctgt
     2281 ctgctgccga tgtgcacgtg ggcgttagaa cggcaaggtg tccacgtcca gccctcggat
     2341 aagcgtgccg atcccgttgg gtcggtgaat gcggcgcctt gggatagcct cacgcccgaa
     2401 caggctgctg gtgcatggca attgacgact gcagagtgga atcaataccg gaaattgatg
     2461 caggggcctg cgcgatacta cgccacagag atgcctccgc tcatggtgct ggcaatgtac
     2521 gcggacacag atcatgagct tgctcactat gctgaattgc ttgcgcag
//
LOCUS       DASUEG010001013         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1362840, whole genome shotgun sequence.
ACCESSION   DASUEG010001013 DASUEG010000000
VERSION     DASUEG010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362840"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VFX11_18965"
     CDS             complement(<1..1712)
                     /locus_tag="VFX11_18965"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX4940764.1"
                     /translation="MKSLTTVVPDQRLRWRPGSGLLLAWLLCCWLLPPLAPLAAGQTD
                     VIVVDDSSTALALPGPLPTALDDPTLPIGAWLQRPFTPDLYGISLQPGPDTYWHRLQL
                     EGRFGDTKPRDWHLVADTHLLRHLEFYLFDGDQLILHQRAGLLDAVDRAGRYSGIHLD
                     FSLRQNQTLTLLIRKQNNGPAILPLTLYSEAAFAVQQRNQYLFWGGVIAVLLALALYN
                     ALVYAMQPGPAYLWYLAFHVTTFLYFSGIHGFGFLLWPDWLQLYLAQHIMTLNFLVIW
                     LVTRFANVFLNAKKNAPWHHRHLRWFGYLCWPGIVASHVVPEYGMIPVFSVVQVAGSV
                     FGISMAVVALRNHYRPAVFFLVSWLFTVAGGAVGMATFTSVLPATFFTLHGFLFGAMA
                     ELMLLSLALADRIRFMEDQMLAKAFEDPLTGAPNFSFFRNRFSDQLEAIQQRHSHLYL
                     LVLDLQGFRELVGLLGPEVLGQAYRNHVDRMTDFIRNQRWAVPIRTLQGEAVFFISLP
                     GGQEMMLADARSAGDDSIQQIMQELHELSDQAIAVSGINSKIGFRIGCAAFHPQSSTI
                     FECFR"
     gene            1971..3161
                     /locus_tag="VFX11_18970"
     CDS             1971..3161
                     /locus_tag="VFX11_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940765.1"
                     /translation="MQKYFRILLPVAFLAAACVVLVILFMARPKAERHAAEERVPMVL
                     VTPVASQSLRIPVLTRGLVLPGNDIPVVAEVSGPVTYVSPNFVTGGFFRKGEVLMRVD
                     DIENEVKIRKTQAMVAQASQALQQAQAEAAARRTGGNDVDLVRNYDQQAKAQFEAAKA
                     ELAALLLAQKNTVLTAPFDGRVRAENLQVGMYLKPGVPLGQIYAVNIAEVRMPLSNQQ
                     AALVDLPTRRSVLAPEGPGVNFSAVYGGTRYHWSGVVLGAEGSMDEFNRLLNVYARIE
                     DPFAADPAQPGRPPLTLGTFVEAEIEGRNFDNVFVVPRRAFRNGSQLWIVTADSRLQR
                     KDVDVLYKGRDFIYVKEGLAEGDQVVLSHLNTAVDGLVVRAQTANPKADAPQPVAQAD
                     ETQP"
     gene            3171..>3629
                     /locus_tag="VFX11_18975"
     CDS             3171..>3629
                     /locus_tag="VFX11_18975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940766.1"
                     /translation="MNTLIEWFVRNRIAANLLMLLFLGAGALSLMDVRSEIIPSVSLD
                     MISVTVPYPGASPEAVEQAIVNPVEAAIYDVEGVKSISSRAVEHLGVVTAELDWGYDS
                     KDILNEIKARVDGIGSFPKDSQRPVISELSVRNLVAYLIISGEADERSLRA"
BASE COUNT          710 a         1138 c         1155 g          626 t
ORIGIN      
        1 cgaaaacatt cgaaaatggt gctggactgc gggtgaaacg ccgcacagcc aatgcgaaaa
       61 ccgatcttgc tgttgatgcc gctgacggcg atggcctggt ccgacagttc gtgcagttcc
      121 tgcatgatct gctggatgct gtcgtcgccg gcggagcggg catccgccag catcatttcc
      181 tggccgccgg gcaagctgat gaaaaacacc gcttcaccct gcagcgtccg gatcggcacg
      241 gcccagcgct ggttgcggat gaaatccgtc atgcgatcca cgtggttgcg gtaggcctgg
      301 cccagcacct ccgggcccag cagccccacc agttcgcgga aaccctgcag atccagcacc
      361 agcaggtaca ggtgggaatg gcgttgctgg atggcttcca gctgatcgga aaatcggttg
      421 cggaagaagg agaaattggg ggcgccagtg agcgggtcct caaacgcttt ggccagcatc
      481 tggtcttcca tgaagcggat gcggtctgcc agcgccagcg acagcagcat cagctccgcc
      541 atcgcaccga acaggaagcc gtgcagggtg aagaaggtgg cgggcagcac actggtgaag
      601 gtggccatgc cgaccgcacc gcccgccacc gtgaacagcc aggacaccag gaagaaaacc
      661 gccggccggt aatggttgcg cagcgccacc accgccatgg agatcccgaa caccgatccc
      721 gccacctgca ccaccgaaaa caccggaatc atcccgtatt ccggcaccac gtgggatgcc
      781 acaatgccgg gccagcacag gtagccgaac cagcgcaggt ggcgatggtg ccagggtgcg
      841 ttcttcttcg cattcaggaa cacgttggcg aagcgggtca ccagccagat caccagaaag
      901 ttcagggtca tgatgtgctg ggccagatac agctgcagcc agtccggcca cagcagaaag
      961 ccgaatccgt gaatgccgga gaaatacagg aacgttgtga cgtgaaaggc cagataccac
     1021 agataggccg gccccggctg catggcgtac accagggcgt tgtaaagcgc cagcgccagc
     1081 agcaccgcaa ttaccccgcc ccagaacaga tactggttgc gctgttgcac cgcgaaggct
     1141 gcctcgctat acagggtcag cggcaggatg gcggggccgt tgttctgctt gcggatcagc
     1201 agggtcagcg tctggttctg gcgcaggctg aaatccaggt ggatgccgct gtaacggccg
     1261 gcccggtcca ccgcgtccag cagaccggcg cgctgatgca ggatcagctg gtcgccatca
     1321 aacagataga attcaaggtg gcgcagcagg tgggtgtcgg ccaccagatg ccagtcgcgt
     1381 ggcttggtgt cgccaaagcg accctccagc tgcaggcgat gccagtaggt gtcagggcca
     1441 ggctgcagtg agatgccgta caggtccggt gtgaaaggac gctgcaacca ggcaccgatg
     1501 ggcagggtgg ggtcatccag ggcagtgggc agcggccccg gcagcgccag cgcggtggag
     1561 ctgtcgtcca cgacaatcac gtcggtctgt ccggccgcca gcggtgccag cgggggtaac
     1621 agccagcagc acagcagcca ggccagcagc aggccggagc ccggccgcca ccgcaggcgc
     1681 tggtccggca cgacagtggt cagtgatttc ataagccgcg acaattggtt ggagcgaatc
     1741 gacgcccagt ctatccttaa actgtagccg atcccccctg cggtaacgta atgccacata
     1801 gcctttacaa atttgtttgt ggcaaccctc ccgcagtttt ttcacttcgg tgtattatct
     1861 tcagcctcgg agcagccggg gtgtcggatg ccccggctgc ccggcaccat ccatccctgt
     1921 cgacacgcgt ggccggcaca cgcagggcgc agcagtagac aacaataaat atgcagaaat
     1981 actttcgaat tctcttgccc gttgccttcc tcgcggccgc ctgcgtcgtt ctggtcatcc
     2041 tgttcatggc ccgtcccaag gcagagcgcc atgccgcgga agaacgggtt cccatggtgc
     2101 tggtcacccc ggtggcctcc cagtctctcc gcattcccgt gctgacccgt ggtctggtgc
     2161 tgccgggcaa cgatattccg gtggtggcgg aagtcagcgg cccggtcacc tatgtgtccc
     2221 ccaacttcgt caccggcggc ttcttccgca agggcgaagt gctgatgcgg gtggacgaca
     2281 tcgaaaatga agtgaagatc cgcaagaccc aggccatggt ggcccaggcc agccaggccc
     2341 tgcagcaggc ccaggcggaa gccgccgccc gccgcaccgg tggcaatgac gtggacctgg
     2401 tgcgcaatta cgatcagcag gccaaggccc agttcgaggc cgccaaggcc gagctcgccg
     2461 ccctgctgct ggcgcagaaa aacaccgtac tgacggcgcc attcgatggc cgcgtgcggg
     2521 ccgagaacct gcaggtgggc atgtacctca agcctggtgt gccgctgggg cagatttatg
     2581 cggtgaatat cgccgaagtg cgcatgccgc tgtccaacca gcaggccgcc ctggtggatc
     2641 tgcccacccg ccgttcggtg ctggcaccgg aaggccctgg cgtcaatttc tccgccgttt
     2701 acggcggcac ccgctaccac tggtccggcg tggtgctggg ggcggagggc agcatggacg
     2761 agttcaaccg cctgctcaac gtttatgccc gcatcgaaga tccgtttgcc gccgacccgg
     2821 cccagcccgg tcgcccaccc ctgaccctgg gcacctttgt cgaggccgag atcgaaggcc
     2881 gcaatttcga caatgtcttc gtggtgccgc ggcgtgcatt ccgcaacggt tcgcagttgt
     2941 ggatcgtgac cgcggacagc cggctgcagc gcaaggacgt cgatgtcctc tacaaagggc
     3001 gcgatttcat ttatgtgaag gaagggctgg cggagggcga ccaggtcgtg ctcagtcacc
     3061 tgaatactgc cgtggatggc ctggtcgtac gcgcccagac tgccaacccg aaggcggatg
     3121 ccccgcaacc cgtggcccag gccgacgaaa cccagccctg aggagcggcg atgaataccc
     3181 tgattgaatg gtttgtacgc aatcgcattg ccgccaatct gctgatgctg ctgttcctgg
     3241 gcgctggcgc cctttccctg atggacgtgc gcagcgaaat catccccagt gtttccctgg
     3301 acatgatttc cgtgaccgtg ccctacccgg gtgcctcgcc ggaagccgtg gagcaggcca
     3361 tcgtcaatcc ggtggaagct gccatctacg atgtggaggg ggtgaaatcc atatcctccc
     3421 gcgcggtgga acacctgggc gtggtgacgg cggaactgga ctggggctac gacagcaagg
     3481 acatcctcaa cgagatcaag gcccgggtgg atggcattgg ttcctttccc aaggattccc
     3541 agcgccccgt gatcagcgag ctgtcggtgc gcaacctggt ggcctacctg atcatttccg
     3601 gcgaggcgga cgagcgctcg ctgcgggcc
//
LOCUS       DASUEG010001014         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1827022, whole genome shotgun sequence.
ACCESSION   DASUEG010001014 DASUEG010000000
VERSION     DASUEG010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827022"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1564)
                     /locus_tag="VFX11_18980"
     CDS             complement(167..1564)
                     /locus_tag="VFX11_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940767.1"
                     /translation="MRITTLMVLVFAICLPVPVQAAVGDPPPPIEKGLLDCSLYRYGS
                     QFDAAVARTLKRMADATYTDPTLKAAFEGATRQDVTDRFYQLELLYRVTDCPQPVVRG
                     YAGARQVVLQVSALQSPVCEQLVSSETLRLSGLVVEPLHVPSGELRCGLARNQVPGLL
                     TLGRLTQAGRSLPGENTVTVIASGPFAEPAPVVGRPPVTPACPSETGRPDPVIEDMLA
                     KQSVVHYDLPRNWKVEPCIDIIGGPSSLGMTCNVANWNNYQQRLYDEFERIMGLAEAS
                     GDPERIYSAAYWLIDQNDPFRMTILSFDYPGQDCDAIRNRILTGWGACIYNKINKAAK
                     DNATDWLQGKKNIPQVKANMTLKSQESVMECFMDRRKYLEYYNDADKLLNDWLRPMPA
                     PEKRQQLMAKVQQRVRSAMANSGYGDCEYYADAVVRLDQVIWRDQYRSGVAQLPVDWD
                     HDFWKSARTCARDSW"
     gene            1758..2192
                     /locus_tag="VFX11_18985"
     CDS             1758..2192
                     /locus_tag="VFX11_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940768.1"
                     /translation="MLRYLLPALVPSWRFFDDIGPSPRIEYCLLADENATPGWQPFQP
                     PPEHIPLPQLFRRLVWNPGRNEALYLLSSCEKILERDALQAQEVIAGRMAQTLRSGEL
                     PPQARYFRFRLLVVEREGDRLVQREQFVSPAFALDQSWGPIP"
     gene            2189..>2694
                     /locus_tag="VFX11_18990"
     CDS             2189..>2694
                     /locus_tag="VFX11_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX4940769.1"
                     /translation="MMDLSITGIESATRLCELLLGLALAQQSIEHLFMPMSDRRLFAL
                     RLLLSLLLAAGLWPTPVLGVLLLTSTWILARCDGPYNGGSDRVAVLMQICLLAVHLCR
                     DLNAPLRWQEVAFGYLAIQIVLSYFMAGWVKIVNPQWRRGRALVDVFAFSAYPQSESL
                     RGWAQWPRL"
BASE COUNT          492 a          841 c          774 g          587 t
ORIGIN      
        1 cggaacgggg ttgatatgcg tcgaggacag ccttgagtat acgcggcata ctcgccccat
       61 tccatgccac tgagcaggac cagttcatga gcctgacctc cggactattc aatccaaaga
      121 accgttttga ctttcacaaa cacagtgtgc aggacgttgc tgcctgctac cacgaatccc
      181 tcgcacaagt gcgcgcgctt ttccagaaat cgtgatccca atccaccggc agctgtgcaa
      241 ctccggaccg gtactgatca cgccagatca cctgatcaag tcgcaccact gcatctgcat
      301 agtattcaca gtcgccatat ccgctgttgg ccatcgcgct gcgaacacgc tgctgcactt
      361 tcgccatcaa ctgctgacgt ttttccggtg ccggcatcgg gcggagccag tcgttgagaa
      421 gcttgtcggc gtcgttgtag tattccagat attttctcct gtccataaag cactccatca
      481 ccgactcctg cgatttcagt gtcatattgg ctttgacctg gggaatattt ttcttccctt
      541 gcagccagtc cgttgcgttg tccttcgctg ccttgttgat cttgttgtag atgcaggcac
      601 cccacccggt aagaatccgg ttccggatgg cgtcacaatc ctggccggga tagtcaaatg
      661 acaagatggt catgcgaaag ggatcattct ggtcgatcag ccagtaggct gccgaataaa
      721 tccgttccgg atcgccactg gcttccgcca accccatgat gcgttcaaat tcgtcataca
      781 gtcgctgctg gtagttgttc cagttcgcaa cgttgcaggt catccccaga cttgagggcc
      841 cccctatgat gtcgatacag ggttccactt tccaattgcg gggcaagtcg taatggacaa
      901 cgctttgctt ggccagcatg tcttcgatca cggggtctgg gcgcccggtc tcgctggggc
      961 atgcgggtgt gaccggtggc cggcccacca ccggcgccgg ttccgcgaac gggccgctcg
     1021 cgatgaccgt cacggtattc tcacccggca ggcttctgcc ggcctgggtc agccgtccca
     1081 acgtcagcaa tcccggcacc tggttgcgcg ccagcccgca gcgcaattcg ccgctgggaa
     1141 cgtgcagcgg ttccactacc aatccggaca ggcgcaacgt ttcgctgctc accagctgct
     1201 cgcacaccgg tgattgcagc gcgctcacct gcaacaccac ctgacgcgca ccggcataac
     1261 cgcgcaccac cggttgcgga caatccgtca cacggtagag cagttcaagc tggtagaagc
     1321 ggtcggtcac atcctgacgc gtggcgcctt cgaacgctgc cttcagggtc ggatcggtat
     1381 aggtggcatc cgccatccgc ttcagcgtgc gggcgaccgc cgcatcgaac tggctgccat
     1441 agcggtacaa cgagcagtcg agcaatccct tttcaatggg cggaggcggg tcacccacgg
     1501 ccgcctgaac aggaaccggc agacagattg cgaagacaag caccatcagg gttgtgatgc
     1561 gcataaatca ttccttggcg atcgggcggg acggggcggg cgtgctgttt gaatcgattg
     1621 cctgcgtcaa gcattcccca attctgttcc cgactcaacg ggcagtgcga ctgattctgc
     1681 cccaagctca attatggatt tcaaaaaccg ctgaattgac taagctctct gcaatcgctt
     1741 accgagtcca cctgcccatg ttgcgctatc tactgcccgc cctggttcca tcctggcgct
     1801 ttttcgatga catcggaccg tcaccgcgca ttgagtattg cctgctggcg gatgaaaacg
     1861 ccacgcccgg gtggcagccg ttccagccgc cgcctgagca tattccactg ccacaattat
     1921 tccggcgact ggtctggaat cccgggcgca acgaggcgct gtatctgctc tccagctgcg
     1981 aaaaaatcct ggaacgcgat gcactgcagg cgcaggaggt gatcgccgga cgcatggcac
     2041 aaacgctgcg gtctggcgaa ttgccacccc aggcacggta ttttcgcttt cgcctgctgg
     2101 tggtggagcg ggaaggtgac aggctggtgc agcgcgagca gtttgtgtcg ccggcgtttg
     2161 cactcgatca gtcgtgggga ccgattccat gatggatctt tccatcacag gcatcgaatc
     2221 cgccacacgc ctgtgtgaac tcctcctcgg cctggcgctg gcccagcaaa gcatcgagca
     2281 tctgttcatg cccatgagcg atcggcgcct gtttgccctg cgcctgctgc tgagcctgtt
     2341 gctggcggcg ggcctgtggc cgacgccggt gctgggcgtt ctgctgctca ccagcacctg
     2401 gatactggcc cgttgcgacg gcccctacaa cggcggctcc gaccgcgtcg cggtgctgat
     2461 gcagatctgt ctgctggccg tgcatctgtg ccgggatctg aatgcgccgc tgcgctggca
     2521 ggaggtggcg ttcggctacc tcgccatcca gatcgtgctg tcgtatttca tggcaggctg
     2581 ggtgaagatc gtgaatccgc agtggcgccg gggtcgggcg ctggtggatg tgttcgcgtt
     2641 ttccgcctac ccgcaaagcg aatccctgcg cggctgggca cagtggccgc gcct
//
LOCUS       DASUEG010001015         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1310230, whole genome shotgun sequence.
ACCESSION   DASUEG010001015 DASUEG010000000
VERSION     DASUEG010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310230"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFX11_18995"
     CDS             complement(<1..834)
                     /locus_tag="VFX11_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4940770.1"
                     /translation="MPVYESLLNPAGDEFKANADQMLKLVQEFRAIEESVMATEAKAK
                     PKFDKRGQLLPRERINRLLDPGSPFLELMSLAGHLMHDDRDGSSAGGGIIAGIGYVEG
                     IRCMVVANNSAVKGGTISPAGLHKSLRLQEIVAQNKLPTITLSESGGANLNYAGEIFV
                     EGARTFANQARLSAAGIPQITVVHGNATAGGAYQPGLSDYIIAVRKRTKMFLAGPPLL
                     KAATGEIAHDEDLGGAEMHAQVSGTAEYLAENDADGIRLAREILRMIPWNDALPTLTK
                     KA"
     gene            complement(837..1715)
                     /locus_tag="VFX11_19000"
     CDS             complement(837..1715)
                     /locus_tag="VFX11_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4940771.1"
                     /translation="MGYRSIFHPELFKDKTIIVTGGGSGIGRCTAHELASLGANVVLI
                     GRKQEKLDKVMAEIREDGGRCSGFACDIRDEESVRGTVSKIVAENKIIHGLVNNAGGQ
                     FPSPLMAISKKGWDAVINTNLTGGFLMAREVFNQSMSSHGGSIVNIVADMWNGMPGMG
                     HSGAARAGMVNFTQTAAYEWGHAGVRVNAVAPGWITSSGMDTYPDSFKAVIKTLKAAV
                     PLGRMGNEAEVSSSICFLLSEGAAFISGTTVRIDGAASQGNVAIYPLQAHKNSKPYDG
                     FHREIVPALFLDDEGK"
     gene            complement(1718..3514)
                     /locus_tag="VFX11_19005"
     CDS             complement(1718..3514)
                     /locus_tag="VFX11_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4940772.1"
                     /translation="MTGNIIRIGCASAFWGDTESAAFQLIRQADLDYLVFDYLAEITM
                     SIMAGARMQQPDAGYATDFVTSVMQPLLPEIAAKGVKIIANAGGVNPKGCRDALQKLI
                     DAAGLQLKVALVLGDDLLPRAPDYRNQGITEMDRGTPLPPFMVSMNAYLGAPAIVAAL
                     QAGADIVITGRVVDSALVLAPLVHEFGWQWNDFDKLAQGSLAGHIIECGAQCTGGNFT
                     DWESVPRYDNMGFPVIECAADGSFVVTKPDNTGGLVSCATVGEQLVYEIGNPAAYFLP
                     DVVCDFTQVNLQQTGENRVAVSGAKGLPPTDQYKVSATYPDGFRITASFILAGIDARR
                     KAERVSAAILKKVSRLFAEKGMADFSRTDVDILGSEATYGAHSRALNSREVVVKIAAS
                     HSDKKALVLFSKEIAQAATGMAPGITGIVGGRPKVSPMIRLFSCLVPKSSVQPVMDIQ
                     GQVTDVAVNNANGYDGVNDTPITQEPGLQTAASVPLVKLAWARSGDKGNHANIGVIAR
                     KPDYLPFIKAALTPAAVARYMQHVLDPQTGKVSAWELPGLHALNFLLENSLGGGGIAS
                     LRSDPQGKAFAQQLLEFPIPVSSTIAQELQVK"
BASE COUNT          714 a         1142 c         1036 g          809 t
ORIGIN      
        1 ggctttcttc gtcagtgttg gcagtgcatc gttccaggga atcatgcgca ggatctcccg
       61 cgccaggcga atgccgtcgg catcattttc tgccagatat tctgccgtgc cggagacttg
      121 cgcatgcatt tccgcgccgc ccagatcttc gtcgtgggcg atttctcctg tggccgcttt
      181 caatagtggc ggacctgcca gaaacatctt ggtgcgcttg cggacggcga tgatgtagtc
      241 cgacaaacca ggctgatacg cgccacctgc cgtagcattg ccatgtacca ccgtgatctg
      301 gggaataccc gccgccgaca gtcgcgcctg attggcaaag gtccgcgcgc cttccacaaa
      361 tatttcaccg gcataattca ggttggcgcc accgctttcc gacagtgtga tggttggcag
      421 tttgttctgc gccacgattt cctgcaggcg cagtgatttg tgcaatcccg ccggcgaaat
      481 ggtgccaccc ttcaccgcac tgttgttggc caccaccatg caccggatac cttccacata
      541 accgatgccg gcaatgatgc caccgccggc actgctgcca tcacggtcat cgtgcatcag
      601 atggcccgcc agcgacatca attccagaaa cggcgaaccg gggtccaata gacggttgat
      661 ccgctcccgt ggcaacagtt gtccgcgctt gtcgaatttc ggtttggctt tcgcttctgt
      721 cgccatcacg gattcctcga tggcgcgaaa ttcctgcacc agcttcagca tctgatctgc
      781 gttggctttg aattcgtccc cggcggggtt cagcaggctt tcatacaccg gcatgattat
      841 ttaccctcgt catccagaaa aagagcggga acgatttccc ggtggaatcc gtcgtaaggt
      901 ttggaattct tgtgcgcctg cagcggatag attgccacgt tgccctgaga ggcagcgccg
      961 tcgatgcgca ccgtcgtgcc gctgataaag gccgcgcctt cgcttaagag aaaacagata
     1021 ctgctgctga cttccgcttc attgcccatg cgccccagcg gcaccgcagc tttcagcgtt
     1081 ttgatcacgg ccttgaagct gtcgggataa gtgtccatgc cgctggacgt aatccagccc
     1141 ggcgccacag cattcacccg cacaccggca tggccccatt cataggcagc cgtctgcgtg
     1201 aaattcacca tgcccgcacg tgcagcgccc gagtgcccca tgcccggcat gccgttccac
     1261 atgtcggcaa caatattgac gatggagccg ccgtggctgc tcatgctctg gttgaatact
     1321 tcccgcgcca tcaaaaaacc gccggtgaga ttggtgttga tcaccgcgtc ccaacctttt
     1381 ttggaaatcg ccatcagggg agacggaaac tggccgccgg cattgtttac caggccgtgt
     1441 atgattttat tttccgccac aattttgctg acggtgccgc gcacggattc ctcgtcgcga
     1501 atatcacagg cgaagccgga acagcggcct ccatcctcgc ggatttctgc catcaccttg
     1561 tccagctttt cctgcttgcg gccgatcagt acgacgtttg cacccagtga cgccagttcg
     1621 tgcgcggtgc aacggccgat gccgctgcca ccaccggtga cgatgatggt cttgtccttg
     1681 aagagttcag gatgaaaaat agaacggtat cccatgatca cttgacctgt agttcttgcg
     1741 cgatggtgga agaaaccgga atcggaaatt ccagcagctg ctgggcgaag gccttgcctt
     1801 gcggatcgct gcgcagactg gcgataccgc cgccgcccag gctgttttcc agcagaaaat
     1861 tcagtgcatg cagccccggc agttcccagg ccgacacttt tccggtctgc gggtccagca
     1921 cgtgttgcat gtatctcgcc acggcggcgg gcgtcagtgc cgccttgatg aagggcaggt
     1981 agtccggctt gcgcgcaatg acgccgatat tggcatgatt gcccttgtcg ccgctgcggg
     2041 cccaggccag tttcaccagc ggtacgctgg cagcggtttg cagcccgggc tcctgtgtga
     2101 tgggcgtgtc attgacgccg tcataaccgt tggcgttatt cacagcgacg tctgttacct
     2161 ggccctgaat atccatcaca ggttgcacgc tgcttttcgg aaccagacag gaaaacaacc
     2221 ggatcatcgg cgacactttt ggccggccgc cgacaatgcc ggtgatgccc ggtgccatgc
     2281 cggtggccgc ttgtgcgatt tcttttgaaa acagtaccag cgcttttttg tcactgtggc
     2341 tggctgcgat cttcactacg acttcgcgcg aattcagcgc ccgggaatgc gcgccgtagg
     2401 tagcttcgct gcccagaata tccacatccg tgcggctgaa atccgccatg cccttttctg
     2461 caaacaggcg gctgactttt ttcagaatcg cggcactgac gcgctctgcc ttgcggcggg
     2521 cgtcgatgcc cgcgaggatg aaagaagccg taatgcgaaa gccgtcggga taggtggcag
     2581 agactttata ttgatctgtc ggcggcaagc ctttggcccc actcacggcc acacggtttt
     2641 cgccggtttg ctgcagattc acctgcgtga aatcgcacac cacatccggc aggaaatagg
     2701 cggccggatt gccgatctca tacaccagct gttcacccac cgtggcgcag ctgaccagtc
     2761 cgccagtatt gtccggcttg gttactacaa agctgccatc cgccgcgcat tcgatcacgg
     2821 gaaaacccat gttgtcgtac ctgggcacac tttcccagtc agtgaaattg ccgccggtgc
     2881 actgcgcacc gcactcgatg atatggccgg ccagactgcc ctgggccagc ttgtcgaaat
     2941 cgttccactg ccagccgaat tcgtgcacca gcggcgccag caccagcgcg ctgtccacca
     3001 cgcgaccggt gatcacgatg tccgcgccag cctgcagtgc ggccacgatc gccggtgcac
     3061 ccagatacgc attcatgctc accatgaaag gtggcagcgg tgtgccgcga tccatttccg
     3121 ttatgccctg attgcggtag tccggcgcac ggggcagcag atcgtcaccc agtaccagcg
     3181 cgactttcag ttgcaggccg gcggcgtcaa tcagcttctg cagcgcatcg cggcagccct
     3241 tgggattcac gccgccagcg ttggcaatga tcttcacgcc cttggcggca atctccggca
     3301 gcagtggctg catgacactg gtgacgaaat ccgtcgcata accggcatcc ggctgctgca
     3361 tgcgggcacc ggccatgatc gacatggtga tttcggcgag gtaatcaaaa accagataat
     3421 ccaggtcggc ctggcggatc agttgaaagg cggcggattc cgtgtcgccc cagaaggcgg
     3481 aggcgcagcc gatcctgatg atgtttccgg tcatgaaagc actactcctg tttgtgggtc
     3541 ctgacgctcg gggcgacggg gcggttgcga aaaaccggcg gatactgcaa agttgcccta
     3601 cactaccaag caagcgcttg gtttgtaaag tacccctatg accactggct ttttggcgtc
     3661 ctttactgta agaaaagccg cccggaccca agaccaccca c
//
LOCUS       DASUEG010001016         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1097843, whole genome shotgun sequence.
ACCESSION   DASUEG010001016 DASUEG010000000
VERSION     DASUEG010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097843"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFX11_19010"
     CDS             <1..767
                     /locus_tag="VFX11_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940773.1"
                     /translation="LLLHTQAQAANNPSVVLRDIHSAQLILQAAASSFHRYQGAEGDP
                     RQLKVLNADLLVLKDSLRAVFQDLADLGMTSELTQLKGHWQEAARNLNTAVSAIGGKG
                     FAEGQVVNDYLRNNHKAATDLRNAYAAVVKLTGVPVNPTVQALRDQTLLMQELCTLYL
                     ELSYATYAYTYRAELGDKDTLDQMAMRFRHGLDQLQVPATGNLPAQLADARAKWQFLE
                     KSFLQYNEKSVPYLVLKFGPVIISDLQQMTATLDPA"
     gene            complement(792..1292)
                     /locus_tag="VFX11_19015"
     CDS             complement(792..1292)
                     /locus_tag="VFX11_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940774.1"
                     /translation="MTIWIIIAVSMLFIVGPILMLRPSPRERRLARIRAVAQQHRVKI
                     QPLFLRRDPVYAATLERNLHLADHDWARYQLVAEEKQTGPSVKGKWVQRRTPQGELVW
                     EAQDIRQTPVPAVDALLARWHAAQSPDFLALELGPRSASLVWNEKGDLAEVEAVCRQL
                     QELLTV"
     gene            1417..1860
                     /locus_tag="VFX11_19020"
     CDS             1417..1860
                     /locus_tag="VFX11_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4940775.1"
                     /translation="MGQYTHILLAADFNQESDIVVKRAKALAGTFGAKLSLVHVLEPI
                     SIAYGGEFPVDLGDLHKELEKQAQTQLAALGDQLGVAAENRYLEVGITEKEILRVAKA
                     HGVDLIVMGSHGRHGLALLLGSTANAVLHHADIDVLAVRVSAKKK"
     gene            complement(1877..2599)
                     /locus_tag="VFX11_19025"
     CDS             complement(1877..2599)
                     /locus_tag="VFX11_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940776.1"
                     /translation="MKIQYTFTLEDLALHYEVDVDREFDLKQARPEAPEWAALGHNQC
                     PNCPLTVKDCAYCPAAVDLVQVVTDFQRLPAIESAKVRVVTPEREYFKETALEEGLRS
                     LMGLIMATSACPILHKLKPNARNHLPFASQDEFIIRSAALYLLRQYFNYREGMRPDWD
                     LQGLISINEELQTLNQAFWQRVHKACESDSNLKALLSFFNLSSSVSYSLEAQLQRIRP
                     IMTDESVSPAIQPESDRERSFY"
     gene            2814..3407
                     /locus_tag="VFX11_19030"
     CDS             2814..3407
                     /locus_tag="VFX11_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940777.1"
                     /translation="MAKGSHLINPGSSAVKPDLDWSQVKETISMLCLAMAQIETTLND
                     SSRSVGELTETFSGMAQDAQTVSQLCTELDSAAKWESRRDELAQTAQRISQQMYRSIV
                     AFQFYDRLSQKLGHVNESLTHLGELISDASRLYNPGEWRRIQDEIRSHYTMECERMMF
                     DLIMQGATVAEALALYRHQFQQTEALRDSNGDEIELF"
     gene            complement(3445..4239)
                     /locus_tag="VFX11_19035"
     CDS             complement(3445..4239)
                     /locus_tag="VFX11_19035"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX4940778.1"
                     /translation="MEVTRLFVHPVKSLGGIGVNAFAIDRFGPVWDRRWLVVDRQGQF
                     ITQRQHARMALVRAALDKDQVTLNAPQRNPLVFSATDFAGGDAVKVQVWRDQCDAVTG
                     PAHFDQWLSDFLGVDCRLVFMPESSRRPVNTDYAQAGETVGFADGFPLLLAAEESLRD
                     LNSRMSITIGMERFRPNLVVAGSAAFAEDGWKRIRVGTMEFRVAKPCSRCAIPTIDPA
                     TAQKQPEVFKTLKAFRQRDGEVYFGQNLLPVGAGSVRVGDRVVILE"
     gene            complement(4255..5655)
                     /locus_tag="VFX11_19040"
     CDS             complement(4255..5655)
                     /locus_tag="VFX11_19040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4940779.1"
                     /translation="MAKLTFWGAAQEVTGSCHLIETEGLRILLDCGMHQGGKQAEKRN
                     GEPFPFDPRRLDAVVLSHGHLDHSGLLPRLFKDGYKGPVFCTEATRNLLAIMLDDAVN
                     LHLRDVEWENQRRKRAGKPPIAPWYDEEDVLHVLEHCVGVPYHQSRAINGHVSLYFRD
                     AGHIIGSAIVELKISEKGAEKTLVFSGDLGNTDVVLMNNPETVSEADLVLMEGTYGDR
                     DHRSMDHTLDELEAIMQQAHQDRGNVLIPAFAVGRTQEMLFHLSALHLKGKLPQQKVF
                     LDSPMAIEVTRLYSEYVELLDAADLKRLSLRFGDDPRKLLPSLTFTQNTEDSMAINRV
                     KGGAVIIAGSGMCNGGRIRHHLKHNLWREECHIVFPGFQAKGTLGRQLVDGEKKVKML
                     GSTFAVKAKIHTLGGFSAHAGQSELLDWAGHFNGKPRFYLVHGEADALQVLQQKLFSA
                     RGISAEVPAEGSSIVF"
     gene            complement(5809..7086)
                     /locus_tag="VFX11_19045"
     CDS             complement(5809..7086)
                     /locus_tag="VFX11_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4940780.1"
                     /translation="MYVDQASKQQLQAALADLESQYAGFQAAKLSLDLTRGKPNSAQL
                     DLSNRLDGILDGQYKGADGTDSRNYGGLDGQPEAKALFCQMLGVKPEETLIGGNASLT
                     LMFQTLNYAHNFGVRGAGSAWNKEGKIKFLCPVPGYDRHFGICEELGIEMIPVAMDDN
                     GPDMDQVEALVKADKLIKGIWCVPRFSNPSGIVYSDQVVERIAKLAKIAGPNFRIMWD
                     NAYCIHAFHDDAQPLANVMDLARKHGTEDSLLIFGSTSKITFAGAGLAFMGASVENLK
                     HFKKHLGIITIGPDKVNQLRHVKFFGDHAGLLDHMRKHAELLKPRFDAVIEHLNKGLK
                     NTDMGSWTVPEGGYFVSFDSRPGLAKEIVRLADAVGVKLTPAGATFPYGKDPADRNIR
                     LAPSFPTLADINKAMEVFVVCVQLASVRQKLAA"
     gene            7300..7375
                     /locus_tag="VFX11_19050"
     tRNA            7300..7375
                     /locus_tag="VFX11_19050"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7333..7335,aa:Asn,seq:gtt)
BASE COUNT         1542 a         2372 c         2125 g         1387 t
ORIGIN      
        1 tgcttctgct gcacacgcag gcccaggcgg ccaacaaccc gtccgtggtg ctgcgggaca
       61 tccactccgc ccagctgatc ctgcaggcgg ccgcctccag cttccaccgc tatcagggcg
      121 ccgagggtga tccccgccag ctgaaagtgc tgaatgcgga cctgctggtg ctgaaggatt
      181 cgttgcgggc ggtattccag gatctggcgg atctgggcat gaccagcgag ctgacccagc
      241 tcaaaggcca ctggcaggaa gcggcgcgca acctgaatac ggccgtcagt gccatcggcg
      301 gcaagggctt tgcggaaggc caggtggtga acgactacct gcgcaacaat cacaaggcag
      361 cgacggacct gcgcaacgcc tacgcggcgg tagtgaagct gacgggagtg ccagtgaatc
      421 ccaccgtgca ggccctgcgc gaccagaccc tgctgatgca ggaactgtgc accctgtatc
      481 tggaactgag ctatgccacc tacgcgtaca cctaccgcgc cgaactgggt gacaaggaca
      541 ccctggacca gatggcaatg cgcttccggc acggcctgga ccagctgcag gtacccgcca
      601 ccggcaacct gcccgcccag ctggcggacg cccgcgccaa gtggcagttc ctggagaaat
      661 ccttcctgca gtacaacgag aaatccgtac cctatctggt gctgaagttc ggcccggtga
      721 tcatcagcga cctgcagcaa atgacggcca cgctggaccc tgcctgaacg gggcagccag
      781 cccgcgccgt tttacaccgt cagcagttcc tgcaactgcc ggcagacggc ttccacctct
      841 gccagatcgc ccttctcgtt ccacaccaga gaggcgctgc ggggccccag ttccagcgcc
      901 aggaaatccg gcgactgcgc cgcgtgccag cgggccagca gcgcgtcgac ggccggtacg
      961 ggggtctggc ggatatcctg cgcttcccac accagttccc cctgcggcgt cctgcgctgc
     1021 acccacttgc ccttgacgga cggcccggtc tgcttctcct ccgccaccag ctggtagcgg
     1081 gcccagtcgt ggtccgccag atggagattg cgttccagcg tggcggcata gaccggatca
     1141 cgccgcagga aaagcggctg gatcttgacc cgatgctgct gcgccaccgc gcgaatgcgc
     1201 gccagacgcc gctcgcgggg cgatggccgc agcatcagga tgggaccgac gatgaacagc
     1261 atcgatacgg caataatgat ccagatggtc atgatgaata cgctccgact gtgcaatctt
     1321 gaccggcaac aagacacatc cgtcgccggc cttgcattat gcaaaggacg cattatctaa
     1381 tagccaacat catttcccta acagaaggac tgttttatgg gccaatacac tcatattctg
     1441 ctggccgccg acttcaacca ggaatcggac atcgtggtga agcgcgccaa ggcactggcc
     1501 ggcacgttcg gcgcgaagct gagtctggtg catgtgctgg agccgatctc gatcgcctac
     1561 ggcggcgaat tcccggtgga cctgggtgac ctgcacaagg aactggaaaa gcaggcccag
     1621 acccagctgg cagccctggg ggatcaactg ggcgtggcgg cagagaaccg ttacctggaa
     1681 gtgggcatca ccgagaagga aatcctgcgg gtagccaagg cccatggcgt ggaccttatc
     1741 gtgatgggca gccacggccg ccatggcctg gcactgctgc tgggatcgac ggccaatgcc
     1801 gtgctgcatc acgccgacat cgacgtgctc gccgtgcgcg tcagcgcgaa gaaaaagtaa
     1861 cgcgacaaaa aagcaatcag tagaacgagc gctcgcgatc cgactctggc tggatggcgg
     1921 gcgacaccga ctcgtctgtc atgatggggc gaatacgctg caactgcgcc tccagcgaat
     1981 aactcacgct ggacgacagg ttgaagaaac tcagcagcgc cttcaggttg gaatcgcttt
     2041 cacacgcctt gtgcacccgc tgccagaacg cctggttcaa cgtctgcaat tcctcgttga
     2101 tgctgatcag gccctgcaga tcccagtcgg gccgcatgcc ttcccggtaa ttgaaatact
     2161 gccgcagcag atacagcgcc gccgagcgga tgatgaattc atcctgcgat gcaaacggca
     2221 aatggttgcg ggcgttgggc ttgagcttgt gcaggatcgg gcaggcgctg gtggccatga
     2281 tcaggcccat caatgaacgc aggccctctt ccagcgcggt ttccttgaaa tactcccgct
     2341 ccggggtcac gacccgcacc ttggcactct cgatcgctgg caagcgctgg aaatccgtca
     2401 ccacctgcac caggtccacc gctgccggac aataggcgca atccttcacc gtcagcggac
     2461 agttcgggca ctgattgtga cccagtgccg cccactccgg cgcctcggga cgcgcctgct
     2521 tcagatcgaa ctcgcggtcc acgtccacct cgtagtgcag cgcgaggtct tccagcgtga
     2581 aggtgtactg aattttcatg ggattccggc cttttacccg ccacatccgg cgtttccttc
     2641 agtgtagcag gcatcggcat tccgccaact tcaggacaat ggggttaacc ggctgaatga
     2701 acaggaaata tgcactctgt cacgacgcac ccgtgacccg tctttcgcct cccccagcaa
     2761 gttggactat gctgattcag gcaaatccgt ttcctgtggg caggagtgag tgcgtggcca
     2821 aaggttccca cctcatcaat cccggttcca gcgccgtcaa acccgatctc gactggagtc
     2881 aggtaaaaga aaccatttcg atgctgtgcc tggcaatggc gcagatcgag accaccctga
     2941 acgacagctc gcgttcggtg ggtgaactga ccgagacctt ctccggcatg gcccaggacg
     3001 cccagaccgt gtcgcaactc tgcaccgagc tggacagcgc cgccaaatgg gaatcccgcc
     3061 gcgacgaact ggcacagacc gcccagcgca tcagccagca aatgtaccgc tcgatcgtcg
     3121 cgttccagtt ttacgatcgc ctgagccaga aactgggcca cgtcaatgag agcctgaccc
     3181 acctgggcga actgatcagc gatgccagcc gcctgtacaa tccgggtgaa tggcgccgca
     3241 tccaggacga gatccgctcc cactacacca tggaatgcga gcgcatgatg tttgacctga
     3301 tcatgcaggg cgcgacggtg gccgaagcgc tggccctcta tcgccaccag ttccagcaaa
     3361 ccgaagccct gcgcgacagc aacggcgacg aaatcgaact gttctgaccg gcgcgggcaa
     3421 tcggcgccct agaaaagacc cctactattc aagaatcacc acccggtccc ccacccgcac
     3481 ggaacccgcg cccaccggca gcaggttctg gccgaaatac acttcgccat cacgctgacg
     3541 gaatgctttc agcgtcttga acacctccgg ctgtttctgc gccgtggcgg gatcaatggt
     3601 gggaatcgcg cagcgggaac agggcttggc gacgcggaat tccatcgtgc cgacacggat
     3661 gcgtttccag ccatcctcgg cgaaagctgc gctgcctgcc accaccagat tgggccggaa
     3721 ccgctccatg ccaatcgtga tgctcatacg gctgttcagg tcgcgcaggg attcctcggc
     3781 agccagcagc agtggaaagc cgtcggcgaa acccaccgtt tcaccggctt gggcgtaatc
     3841 ggtattcacc ggacggcggg acgattccgg catgaacacc aggcggcaat ccacacccag
     3901 aaaatcactc agccactgat cgaaatgggc ggggccggtg acggcatcgc actgatcgcg
     3961 ccatacctgc accttcaccg cgtcaccgcc agcaaaatcc gttgcagaaa acaccagcgg
     4021 attgcgctgc ggcgcgttca gagtgacctg atccttatcc agcgccgcac gcaccagcgc
     4081 catgcgagca tgctggcgct gggtgatgaa ctggccctgc cggtccacca ccagccagcg
     4141 ccggtcccac accggaccaa agcggtcgat ggcaaatgcg ttcacaccga tgccccccag
     4201 ggatttcacc ggatgtacaa acagacgagt gacttccatt acaccttccc ccgcttaaaa
     4261 aacgatgctg ctgccttcgg ctggcacttc cgcactgatg cccctggccg agaacagttt
     4321 ctgctgcaac acctgcagcg cgtcggcctc gccgtgcacc agatagaacc ggggcttgcc
     4381 gttgaaatgg cccgcccagt ccagcagttc actctggcca gcatgggccg agaatccacc
     4441 cagtgtatga atcttcgctt ttaccgcaaa cgtggacccc agcattttca ccttcttctc
     4501 gccatccacc agctgccggc ccagcgtgcc tttggcctga aaaccgggga acacgatatg
     4561 gcattcctcg cgccacagat tgtgcttcag gtgatgacgg atacgaccgc cgttgcacat
     4621 cccgctgccc gcaatgatca ccgctccgcc cttgacgcga ttgatggcca tggaatcttc
     4681 ggtgttctgc gtgaacgtca gggacggcag caacttgcgg ggatcgtcac cgaaacgcaa
     4741 cgacagacgc ttcagatccg cggcgtccag cagctccacg tattcgctgt acagacgcgt
     4801 cacttcaatc gccatcgggc tgtccagaaa caccttctgc tgcggcagct tgcctttcag
     4861 atgcagtgcg ctcaggtgaa acagcatttc ctgggtccgg cccaccgcaa acgccgggat
     4921 cagcacattg ccgcgatcct ggtgcgcctg ctgcatgatg gcttccagct catccagggt
     4981 gtgatccatg ctgcggtgat cgcgatcgcc gtacgtgcct tccatcagca ccagatcggc
     5041 ctcgctcacg gtttccggat tattcatcag caccacatcg gtattgccga ggtcgccgga
     5101 aaacaccagg gtcttctccg cgcccttctc cgagattttc agctcaacga tggccgaacc
     5161 gatgatatgg cccgcatcgc ggaaatacag cgacacatgc ccattgatcg cgcgggactg
     5221 gtgataagga acgccgacac aatgttccag cacatgcagc acgtcctcct cgtcgtacca
     5281 gggcgcgatg ggcggcttgc cggcgcgctt gcggcgctgg ttttcccact ccacatcacg
     5341 caggtgcaga ttgacggcat cgtccagcat gatcgccagc aggttgcggg tggcctcggt
     5401 gcagaatacc gggcccttgt agccgtcctt gaacaggcgt ggcagcaaac cggaatggtc
     5461 cagatggccg tgggacagca ccaccgcatc cagccggcgc ggatcgaagg gaaagggttc
     5521 gccgttacgc ttttccgcct gcttgccgcc ctgatgcatg ccgcaatcca gcaggatacg
     5581 caggccttcc gtttcaatca ggtgacagga accggtgact tcctgggcag cgccccagaa
     5641 ggtgagtttg gccatggtct ggtaagcccc gttgcattaa tgatgggcac acgataccac
     5701 agcctccgac cctggaaatt gagcaaaacc aatgagcgga tgcggaaaac gaaaaagggc
     5761 gaacccggtg tggattcgcc ctcttttgcg atgcttttga aagtgggatc aggccgccag
     5821 tttctgccgc accgacgcca gctgcacgca caccacgaac acttccattg ccttgttaat
     5881 gtccgccagc gtggggaagc tgggggccag acggatattg cggtccgccg gatcctttcc
     5941 atacgggaag gtggcaccgg ccggggtcag tttcacaccc accgcgtccg ccagccgcac
     6001 gatttccttc gccagaccgg gacgggaatc gaacgacacg aaatagccgc cttccggcac
     6061 ggtccagctg cccatatccg tgtttttcag ccccttgttc aaatgctcga tcaccgcatc
     6121 gaaacggggt ttcagcaatt ccgcgtgctt gcgcatgtga tccagcaaac cggcatgatc
     6181 accgaagaac ttcacatggc gcagctggtt cactttgtcc gggccgatgg tgatgatgcc
     6241 caggtgtttc ttgaaatgct tgaggttctc gaccgatgcg cccatgaacg ccagacccgc
     6301 gccggcaaag gtgatcttcg aggtggaacc gaaaatcagc agggaatcct cggtgccatg
     6361 cttgcgcgcc agatccatca cattggccag cggctgcgcg tcgtcgtgga aggcgtggat
     6421 gcaatacgcg ttgtcccaca tgatgcggaa attggggccg gcaattttgg ccagtttcgc
     6481 aatacgctcc accacctgat cgctgtatac gatgccggac ggattggaga aacgcggcac
     6541 acaccagatg cccttgatca gcttgtccgc cttcaccagc gcttccacct gatccatgtc
     6601 ggggccgttg tcgtccatcg ccaccgggat catctcgatg cccagttcct cgcagatgcc
     6661 aaagtggcgg tcatagccgg gcaccgggca caggaacttg atcttgccct ctttattcca
     6721 cgccgagcct gcaccgcgca cgccgaaatt gtgggcgtag ttcagggtct ggaacatcag
     6781 ggtgaggctg gcattgccgc cgatcagtgt ctcttcgggc ttcacgccca gcatctggca
     6841 gaacagagcc ttggcttcgg gctggccgtc gaggccgccg taattacggc tgtcggtgcc
     6901 gtcggcaccc ttgtactggc catccagaat accgtccagg cggttggaca gatccagctg
     6961 cgcgctgttg ggtttgccac gggtcaggtc caggctcaat ttggcggcct gaaacccggc
     7021 atactggctt tccagatcgg ccagcgcagc ttgcaattgt tgtttcgacg cttgatcaac
     7081 gtacaaggca catcccctct tgtgaagaca tcaacgggcc atttgagacc cgacactata
     7141 gtgtgtcgga atatgaatgc aagcctgcac agacggggta atcgccgttt tgcgaaccgc
     7201 ccgtggccac agcgggaacg gctaacctgt tgaaagcttg ctgaaaaaaa ggagttgacc
     7261 aacccgggca accccattag tatacgcggc ctgtaccgat ccacagtagc tcagtcggta
     7321 gagcaagcgg ctgttaaccg ctaggtcgta ggttcgagcc ctacctgtgg agccaaatac
     7381 gaaaaagggg gaaccgaatg gttccccctt tttattggct gcgtat
//
LOCUS       DASUEG010001017         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1473344, whole genome shotgun sequence.
ACCESSION   DASUEG010001017 DASUEG010000000
VERSION     DASUEG010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473344"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFX11_19055"
     CDS             complement(<1..517)
                     /locus_tag="VFX11_19055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX4940781.1"
                     /translation="MTILVVGASGLIGGEILKALRQAGYMARGASRNRPHEETPASWL
                     ELPFAELEKAQAWLPYLQDVQVVVNCLGIISEQQAGDFDRLHSQIPRALFAACEQSGV
                     QQIIQLSALGASLEASTPYWRSKAEADTALLSRPLRATVIRPSLVYGEGGASTRLFTL
                     LSTLPVLCLPYA"
     gene            complement(514..1056)
                     /locus_tag="VFX11_19060"
     CDS             complement(514..1056)
                     /locus_tag="VFX11_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940782.1"
                     /translation="MTSVLWLLVGQGMLGASDVLINHEWRERLPQQATAATEQLLHGV
                     RELLYALVFAGLAWCLWHGTWAWLFALVLLTEIVLTGWDFVQEDRTRVLSATERLMHL
                     VLSMGGGAYCALLIPHLLQWAQQPTGLVVVHYGAVTWLLSGLAIGVFSWGVRDLQAGF
                     RLRKKGNIARQVPALIEASV"
     gene            complement(1053..1463)
                     /locus_tag="VFX11_19065"
     CDS             complement(1053..1463)
                     /locus_tag="VFX11_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX4940783.1"
                     /translation="MHSLAGMVLAWAPTWLLSELYHQPIDLAFWQTTAPAAARAQQLW
                     WISLFGVTVQSLGLWMWALVYLGQRYRSRFAWGALIAGIVIWAPQDLFISLQADAWLH
                     VWVDLIAVFSMLPPLLWLCWHDGHQPLSGVEQPS"
     gene            complement(1530..1952)
                     /locus_tag="VFX11_19070"
     CDS             complement(1530..1952)
                     /locus_tag="VFX11_19070"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX4940784.1"
                     /translation="MAALTLFFDGHCPFCASEMARLQQWDRYGALAFVDIASPHFEPA
                     SLGVTAADLDHEIHSQDGNGKLLIGIDSLLAAYTLVGKRWLVWPLAVRPLRPAFAALY
                     RSFARNRYRISAWLGYRQAPSCTDGVCGIGNPFFRGHK"
     gene            complement(1977..2504)
                     /locus_tag="VFX11_19075"
     CDS             complement(1977..2504)
                     /locus_tag="VFX11_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEX4940785.1"
                     /translation="MSLSPTMQKYILHWGEMGTRWGVNRTVAQIHALLFLSNQPLTAE
                     TIAETLTVARSNVSTSLKELQSWGLIKVTHVLGDRRDHFVALQDVWEIFRVIIEERKR
                     REIDPTLTVLRECALEAESDQSLEPATRVRMDEVLAFLEMLASTYDDYKHLPPVTLQK
                     MLKMGGKVARFLGSE"
BASE COUNT          653 a          837 c          752 g          495 t
ORIGIN      
        1 gtgcatacgg caggcaaagt accggtaacg tactcagcaa agtgaacagt cgcgtgctgg
       61 cacctccttc gccgtaaacc agagagggcc gaatcaccgt cgcgcgcaaa ggccgcgaca
      121 gcagagctgt gtcggcctcc gctttgctgc gccaatacgg agtgctggcc tcaaggctgg
      181 cccccagtgc agacaattga atgatttgct gaacaccact ctgctcacag gcagcaaaca
      241 gcgcccgcgg gatctggctg tgaagccggt cgaaatcgcc tgcctgctgc tcgctgatga
      301 tgccaaggca gttaacgaca acctgcacat cttgcaaata tggcaaccac gcctgagcct
      361 tttccagttc ggcaaatggc aattcgagcc agcttgctgg cgtttcctcg tgcggtcgat
      421 tccggctggc accgcgagcc atgtaaccgg cttggcgtaa tgctttcaag atttcaccgc
      481 caatcaaacc gctggcaccg acaacgagaa tggtcatacc gacgcctcga tgagtgcggg
      541 cacctgcctc gctatgttgc ccttttttcg caggcgaaag cccgcctgca gatcgcgaac
      601 gccccaggaa aatactccga tggccaagcc gctgagcagc cacgtcaccg cgccgtaatg
      661 gaccaccacc aagccggtcg gttgttgcgc ccactgcagc aagtgcggaa tcagcaaagc
      721 gcaataagca ccgccgccca tgctgagcac cagatgcatc agtcgctcgg tcgctgacag
      781 cacccgcgtg cggtcttctt ggacaaagtc ccagcccgtc aaaacaattt cagtcaacag
      841 gacaagagcg aacagccatg cccatgtgcc gtgccacagg caccaagcca aaccggcgaa
      901 tacgagtgcg tacagcaact cacgcacacc gtgcagcaac tgctcagtcg cggcagtagc
      961 ttgttgcggc aagcgctcac gccactcgtg attgatcaaa acatccgacg cgccgagcat
     1021 gccctgcccg accaatagcc agagcacact ggtcacgatg gctgctccac tccactcaac
     1081 ggctgatgac cgtcatgcca gcacagccac agcagtggcg gcagcatgct gaacacggca
     1141 atcagatcca cccaaacgtg cagccaggca tcggcttgta gcgagatgaa cagatcctgc
     1201 ggcgcccaga ttacgatgcc agcaatcaac gcgccccaag caaagcgact gcgatagcgc
     1261 tggccgagat acaccagcgc ccacatccag agccccagcg actggaccgt gacgccaaac
     1321 aaactgatcc accacagttg ttgggcccgg gcagcagcag gtgcggtggt ttgccagaag
     1381 gcgagatcga tgggctgatg gtagagctcc gacagcaacc aagtcggcgc ccaggccaac
     1441 accatgccgg ccagcgaatg cacgactgcc gcagcgtaca gccattgaat gcaaaaccag
     1501 cgcaacgaaa acgaggcttg tgctttcatt tatttatggc ctctgaagaa cggattgccg
     1561 ataccgcaga cgccatctgt gcaggacggc gcttgccggt aaccgagcca agccgaaatc
     1621 cgataacgat tgcgagcaaa gctgcggtag agcgccgcga aagcaggacg cagcggtcgc
     1681 actgccaatg gccaaaccag ccagcgcttg ccaaccaagg tgtacgccgc cagcaggcta
     1741 tcgatgccga tcagtagttt gccgttgcca tcttggctgt ggatttcgtg atccagatcg
     1801 gcggcggtga caccaagact ggccggttcg aaatggggag aagcgatgtc gacgaatgcc
     1861 aacgcaccat agcggtccca ctgctgcaag cgggccattt cggacgcgca gaatgggcag
     1921 tggccatcaa agaaaagcgt aagtgcggcc atgctatgac tccttctaga gaccggctat
     1981 tcggaaccga gaaaacgcgc aaccttgcca cccatcttga gcattttttg cagcgtgacc
     2041 ggcggcaggt gtttgtagtc gtcgtacgtg ctggcgagca tttccagaaa cgccaacacc
     2101 tcatccattc gcacacgcgt tgccggttcg aggctttgat cggactctgc ctccaaggcg
     2161 cattcgcgca gcacggtcag cgttggatcg atctcgcgac gcttccgctc ttcgatgatg
     2221 acccggaaga tttcccagac atcctgcaga gcgacaaagt ggtcgcgccg gtcgcccagc
     2281 acatgggtca ccttgatcag cccccagctt tgcagctctt tcaggctggt gctgacgttg
     2341 gaacgggcca ccgtcagcgt ctcggcgatg gtctcggcgg taagcggctg gttgctcaga
     2401 aacagcagcg catggatttg cgccaccgtg cgattgaccc cccaccgggt gcccatttcg
     2461 ccccagtgga ggatgtactt ctgcatcgtt gggctgagtg acatggcaat tctctaattt
     2521 ctgtctcgac agaaattaga gaacaaacag aaaacccagt caagcgcttt cgctgctttg
     2581 tgcaaaaagt acacggatta caggttatat ttgccaaaat taggtagcga gtacttacat
     2641 tcagacgctt tagctaccta aacaagtggc tgaaccaagc tcagacaaac gtcagatacg
     2701 gcacgatgct gccggcgaac tgggtcagga tgcggaa
//
LOCUS       DASUEG010001018         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1724521, whole genome shotgun sequence.
ACCESSION   DASUEG010001018 DASUEG010000000
VERSION     DASUEG010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724521"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFX11_19080"
     CDS             <1..996
                     /locus_tag="VFX11_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HEX4940786.1"
                     /translation="VDAEAIDDGANEEDEQRDYLGEMNNLTVMMITGAKKIAQERLSE
                     ADRTLIKECLIAGLHASREQGAQHAVIADVVDAMDARLRDADLDEPQRIRLREMRMAL
                     NRWTTGTHGHFFNRYGRSWPEVDCTIVDMGILTGNDNDDMLAVALISLINTITGLGEK
                     YQHTHGARETIVLIDEGHVITKNPVLAKPLVFGLKTWRKLGIWLWQATQNLKDYPDEA
                     QVMLALAEWWICLRMESAQEIEHIAKFKTLTQEDRHLLASARKQDGAFTEGVILGSKI
                     KALFRVVMPALPLVLSMTDPDQKKAIAELARINGVSEIGAAKLMAQQIIEKRETA"
     gene            993..1460
                     /locus_tag="VFX11_19085"
     CDS             993..1460
                     /locus_tag="VFX11_19085"
                     /inference="COORDINATES: protein motif:HMM:NF019152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1525 domain-containing protein"
                     /protein_id="HEX4940787.1"
                     /translation="MNPRLFIIEQGHSFLKACSVGLLIYSQPVLAEPVHVTFFGNNQY
                     QPTGIEAVEREGVIVTVYNLDSQVNLEKHLAEGLPADDLEKATAIARSRVAQISQQEM
                     VSLFTGILRAREWGIERFPAIVFGRGESVVYGVTDLEQALMLWRDLQAAGIGQ"
     gene            1496..2929
                     /locus_tag="VFX11_19090"
     CDS             1496..2929
                     /locus_tag="VFX11_19090"
                     /inference="COORDINATES: protein motif:HMM:NF018536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HEX4940788.1"
                     /translation="MTIATVSVPARAAGEDQSGFTIVDLVESGVRGLPDCLNYCVVGV
                     CIHLRVRLLSIQILISPRVEHNTPEFVVSAYAAGKQQPWMEWREVFGPAQESSSNGML
                     ALITSSGRMGGYGTYQEETQIYDRQEIFKEVDVLGHPLSMLPTLLANGDISDRMPRAT
                     QQANLVQMIDEANQEESPTELGTFNDAMDSANEIVDTAGFPAIFLDPALLRVFSYMDT
                     ANQIRDSVNTVAEAMNLLQEVSDLISSPPGISGSFRTDNLMCPNSVLPFTPYYLSSND
                     LFGWRLGIPDRIFHAGDIAQAMVPLMSNRKVLGTATGGMPGIGEGTWAGLYPRMGFVR
                     NSHDGKTASVVAQRGLDLVLANRGSDHRGHVTLFSPGFPNRNDHRVLPYVFNASGVDG
                     GLWQPVFPAGKHQCTDNLYQDKTMADIANDRSAPRSNKLRYAWTFWRRYNCCLQRRGR
                     LIESISIPPVCLTANLIDDPAPSSEAY"
     gene            2948..>4243
                     /locus_tag="VFX11_19095"
     CDS             2948..>4243
                     /locus_tag="VFX11_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940789.1"
                     /translation="MVAIFKARFKAVLVLLALSVAMLMVPSPAPRAEPLTQYVDDFFY
                     ELGGGRAIPRGAAGYVTYRIGARFTITPGYSCGNFSLEQNLEQALNRIRTQVQGLPDQ
                     LSMAATNMISSLPMYLVKNYASDIYGILMWNLDQSIELFRFQYKTCETLEAEIMANEK
                     DYNPYAAATRAAVLNQYIWGGENGQTIDETARETQTNPGRRGFNFLGDGRGTAARPIP
                     LKHDLMVLAYNNRLGRVANPLDVSAPTTHQNEAMVQTWRTPQHAADWLVAATGEFWLV
                     VDSSAPKESAPGVGLRPEIDMLTDNYEIAITNAVQLNNFDDLDALELGGVPNKLRLTD
                     RVIESIQALKGDKQAIAIERLASDTAVAIVKNKVDLATTLLRAAVQDPDVATSQIASV
                     AGQVASQARTWLREEMEEISQMMAIDRMGPAATPVIIMNR"
BASE COUNT          965 a         1130 c         1246 g          902 t
ORIGIN      
        1 gtcgatgctg aggcaatcga tgatggtgca aatgaagagg acgagcagcg ggattatctg
       61 ggcgagatga acaatctcac cgtgatgatg attacgggtg ccaagaagat agcgcaggaa
      121 cgactatcag aggcagatcg cacactcatc aaggagtgcc tgattgcagg actgcacgcc
      181 agtcgggaac agggtgccca acatgcggta attgccgacg tggtcgacgc catggatgcc
      241 cgtttacgcg acgcggatct ggacgaaccc caacgcatcc gtctgcgtga gatgcgcatg
      301 gccctgaatc gctggacaac cggaacgcac ggccacttct tcaatcgcta tggtcggtcg
      361 tggccggaag tagattgcac tatcgtggac atgggcattc tgactggtaa tgacaacgat
      421 gacatgttgg ccgtggcgtt gatttcactg atcaatacca tcaccggcct gggggaaaaa
      481 tatcagcata cccacggtgc acgcgaaacc attgtgctga tcgacgaagg ccacgtcatt
      541 accaaaaacc ccgtgctggc gaagccgctg gtgtttggat tgaagacctg gcgaaaactc
      601 ggtatctggt tgtggcaggc gacgcagaat ctcaaagatt atcccgacga agcccaggtg
      661 atgctggcgc tggcggaatg gtggatttgc ttgcggatgg agtcggccca ggaaattgag
      721 catatcgcca agttcaaaac gctgacacag gaagatcgcc atttgctggc ctccgcccgc
      781 aagcaggatg gcgccttcac tgaaggcgtc atcctgggtt ccaagatcaa ggcactgttt
      841 cgggttgtga tgcctgcact gccgctggtg ttgtcgatga ctgatccgga tcagaaaaag
      901 gccatcgcgg aactcgccag aatcaacggt gtgtcggaaa tcggagcggc caagttgatg
      961 gcgcagcaga tcatcgaaaa gagggaaacc gcatgaaccc gagattgttc atcatcgaac
     1021 aaggccattc gtttctgaag gcttgctctg tcggacttct gatctattcg cagcctgttt
     1081 tggcggagcc tgttcacgtg actttttttg gcaacaacca ataccagccc actggtatcg
     1141 aggcagtgga gcgtgaaggt gtaattgtca ccgtctacaa ccttgattca caggtcaacc
     1201 tggaaaagca tctcgccgaa ggattgcctg ccgacgatct ggagaaggcc actgcgatcg
     1261 cacggtcgcg ggtcgcacag atttcccagc aggagatggt gagtctgttt accggcattc
     1321 tcagagcaag agagtggggt attgaacggt ttcccgcgat cgtcttcggt cggggtgagt
     1381 ccgtggtata cggtgtcacg gatctggagc aggcgctgat gctctggcgc gatctgcagg
     1441 cagcggggat aggccaatga aatcgcaaat tcgcattcta ctgctggcgg tttgtatgac
     1501 aatcgccacc gtgtctgtgc cggcccgcgc cgccggtgaa gatcaaagcg gattcaccat
     1561 tgtcgatctg gtggagtcgg gcgtcagggg attgcctgac tgcctcaact actgcgtcgt
     1621 gggtgtctgc atccacctgc gtgtgcgatt gctgtcgatc cagatcctga tttctccccg
     1681 cgtggaacac aatacacctg aattcgtggt gtccgcctat gcggcgggca aacagcaacc
     1741 gtggatggag tggcgagagg tgttcgggcc agcgcaggag tcttcaagca acggtatgtt
     1801 ggccctgatc acgtccagtg gtcgcatggg tggctatggc acgtatcaag aggaaaccca
     1861 aatttatgat cgacaggaaa ttttcaagga agtggacgta ctgggacatc ccctgtcaat
     1921 gttgcccaca ttgttggcaa atggcgatat ttcggatcgg atgccaagag cgacccagca
     1981 agccaacctc gtgcagatga tcgacgaggc caatcaggag gaatccccca ccgagctggg
     2041 cacattcaac gacgcgatgg attctgccaa tgagattgtc gataccgcag gatttcctgc
     2101 gatatttctc gatccggcat tgttgcgtgt gttctcttac atggacaccg cgaatcagat
     2161 ccgggattcc gtcaacacgg tggcagaggc catgaacctg ttgcaggaag tgtcagacct
     2221 gatcagctcg ccaccgggca tttccggaag tttccgcacg gacaatctga tgtgtcccaa
     2281 cagcgtgttg ccgttcacgc cctactacct gagttcaaac gatctgtttg gttggcgctt
     2341 gggtattccc gatcggatat ttcatgccgg cgacatcgct caggccatgg tgccactcat
     2401 gagcaaccgg aaagtgctgg gaacagctac cggcggcatg ccagggattg gcgaaggtac
     2461 ttgggctggc ttgtatcccc gcatgggatt tgtccgcaat tcccacgacg gcaagactgc
     2521 cagtgtggtg gcgcagcgcg gcctggatct ggtgctggcc aatcggggca gtgatcatcg
     2581 cgggcatgtc acgctgttca gccctggctt cccaaatcgc aacgatcacc gggtgctgcc
     2641 ctacgtcttc aacgcgagtg gtgtcgatgg tggcttgtgg caacccgttt ttcctgccgg
     2701 gaaacaccag tgcaccgaca atctttacca ggacaagacc atggccgaca tcgccaatga
     2761 tcggtcggca ccccgcagca acaaattgcg ttacgcctgg acgttctggc gccgttacaa
     2821 ctgctgtttg cagcggcgcg gacgtctgat cgagagcatc agcattccac cggtttgtct
     2881 gaccgccaat ctgatcgatg acccggcgcc cagcagtgag gcgtactgac ccatgaagga
     2941 ttccactatg gttgcaatct ttaaagcacg atttaaagca gtgctggtgc tactggcatt
     3001 gtcggtggca atgctgatgg tgccaagccc tgcaccacgc gctgaaccac tgacccagta
     3061 cgttgatgac tttttctacg aactgggagg cgggcgggcc attccccgag gtgcggctgg
     3121 atatgtcact taccggatag gggcacggtt caccatcacg ccgggatatt cctgcggcaa
     3181 tttctccctc gaacagaatc tggagcaggc gctgaatcgc atccgcaccc aggtgcaggg
     3241 tctgccggat cagctgagca tggccgctac caacatgatt tcgtcgttgc cgatgtatct
     3301 cgttaaaaac tatgcgtctg atatctacgg catcctgatg tggaatctgg atcagtccat
     3361 cgaattgttc cgcttccagt acaaaacctg cgaaacgctg gaagcggaaa tcatggcgaa
     3421 cgaaaaagac tacaacccct atgccgccgc tacacgggct gcggtgttga accagtacat
     3481 ctggggcggg gaaaatggcc agacgatcga tgaaactgcc cgtgagactc agacgaatcc
     3541 gggtcggcgg ggcttcaatt ttctaggtga cggacgcggt accgcagcgc gaccgatccc
     3601 gctcaaacat gatctgatgg tgcttgccta caataaccga ctgggtcggg tggcgaatcc
     3661 tctcgatgtg tcggcaccga ccacccacca gaacgaagcc atggtacaga catggcgtac
     3721 accccaacac gcggcggatt ggctggtggc cgcgaccggc gaattctggt tggtagtcga
     3781 tagcagtgca ccgaaggaat ctgcaccggg tgtggggctg cggccagaaa tcgatatgct
     3841 cacggacaat tacgagatag cgattaccaa tgccgtgcaa ctcaataatt tcgacgatct
     3901 tgatgcattg gaacttggcg gagttcccaa caagctgcgc ctgacggatc gtgtcatcga
     3961 atccatacag gcactgaagg gcgacaaaca agccatagcc atcgaacgcc tggcttcgga
     4021 cacggccgtg gctatcgtca agaacaaagt ggatctggcc accacgttat tgcgtgctgc
     4081 tgtacaggat ccggatgtgg ctacctcgca gattgccagt gtggcagggc aggtggcatc
     4141 ccaggcccgc acctggttgc gagaagagat ggaagagatc agccagatga tggccatcga
     4201 tcgcatgggg ccagccgcca cacctgtgat catcatgaat cga
//
LOCUS       DASUEG010001019         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1672192, whole genome shotgun sequence.
ACCESSION   DASUEG010001019 DASUEG010000000
VERSION     DASUEG010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672192"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..789
                     /locus_tag="VFX11_19100"
     CDS             46..789
                     /locus_tag="VFX11_19100"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4940790.1"
                     /translation="MKSNKIHTKILAVVLGASALSANAGVISYTESFDFSALSFSDSE
                     FGSNDNATTSVTRSQNISLAGFDASLGTLTGVSLSFGSDWSLATKVTASDTNNNWGTE
                     YTNGTSLATSTLSIDLLNPAGPGDDKYNVQTAYCSGTGVYSAACTGKSASAGDFNGSF
                     YLDNINLGAFIGGNVLLNLVQELTAEVTNCDSDSSCYAWNFNNGWAGDITISYVFDTV
                     NVPEPATLALFGLGLIGLGLTRRKVKNQG"
     gene            1189..>3038
                     /locus_tag="VFX11_19105"
     CDS             1189..>3038
                     /locus_tag="VFX11_19105"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4940791.1"
                     /translation="MLSKRTLREWISSLPVLLLIVMVMAISLNEKMYSQMLRVGEQLF
                     GNYYILRVDIPTPSCDPKQDIDKALEEEIHKQKAAQQSDEFGGLFGDSAIDTDALRVS
                     LEGQRQACVQQHEMAKTNQERVTDSIRAFRVLDHAMLAIGHIGRDSQQLILLALIFIC
                     GLTATMTNHHISLRPLQNANDYRISGIAQTIANLSLAISAWEYLQIGANAGVPMQNES
                     TRWGYVIVFCLMTGISLLQSLFVPKSAAPGGSIGKALLSIPLYAYATFGAAYYFLIAS
                     GNKQGLVLHVDRIMDAADLFTKISLLIWAGLLIKQTHIGELVFRIFKPWRLPPELLAF
                     VAIFLMAVPTAYTGASGAIIVAMGGVVYNELRRAGARRNLALATTAMTGSLGVVLKPC
                     LLVLIIAFLNKEVTTDDLFHWGTRVFIMTSFIFFVIALIAREEKLSVAPTREALVPSL
                     KAFIPLFPYVILVAITLLLFYVLLDVKLDENTAAIMVPMIVLVMVIYEKVMGKKRLEE
                     QETYHDEERQTTAEGAIRVATSSTAELMGGLMLLIALSMSIGGVIEETHIIQDAAEHG
                     GLFHNQWTVMTALILVLMMIGMSGMEPFGAVILVSGSLAQVAYKFGIDPI"
BASE COUNT          655 a          919 c          827 g          637 t
ORIGIN      
        1 gcggacaatt tgcaatcagc tcttactttg ggaatggggc aacttatgaa atcgaacaaa
       61 attcatacaa aaattctggc cgtagtgctg ggtgcatctg cactgagtgc caatgccggt
      121 gtcatcagct acaccgaaag ctttgacttc agcgcactgt cgttcagcga cagcgagttc
      181 ggctcaaacg acaatgcaac caccagcgtc acccgcagcc agaacatctc cctggccggc
      241 ttcgacgcca gcctgggcac gctgaccggc gtcagcctga gcttcggttc cgactggagc
      301 ctggccacca aggtcaccgc cagcgacacc aacaataact ggggcaccga gtacaccaac
      361 ggtacttccc tggccacctc gaccctgtcg atcgacctgc tgaacccggc tggtcctggc
      421 gatgacaaat acaacgtaca gacagcctac tgctccggca caggcgttta cagcgcagcc
      481 tgcacaggca agagcgcatc ggccggtgac ttcaacggct ccttctacct ggacaacatc
      541 aacctgggcg cctttattgg cggcaacgtt ctgctgaacc tggtgcagga gctgacggct
      601 gaagtaacca actgcgacag cgattccagc tgctacgcct ggaacttcaa caatggctgg
      661 gctggcgaca tcaccatcag ctacgtcttc gataccgtca acgtaccgga acctgccaca
      721 ctggcgctgt tcggtctggg cctgatcggc ctgggtctga cccgccgcaa ggtgaaaaac
      781 cagggctgat tgtttccctt ccaaaggcga catgatcaaa ccagaaaggc acccccgggt
      841 gccttttttt atttgtaaag tctggataac aaatttttgc accggggtta tcgcatggcc
      901 gtccggcacc cggttatact gcagcgcccg tgccgttggc agatttgcta ttgtgccggg
      961 tttggcgggt tatatacggc caatttgcag ggaaattctg gttctgagcg acaactttct
     1021 gcgcatattt gtcgcgtcaa aaaggcggac ttcacctaga atcggcccac tctgagggct
     1081 taccggggcc tgcaaattgc ctttatccgc gccgtattga cagccggggt agggctgttc
     1141 aaaacaacaa atcagatgga ctacaacaac taaacaaggt gcgcactcat gttgtcgaaa
     1201 cgcacattgc gggaatggat ttcgtcttta cccgtgctgc ttctcattgt gatggtcatg
     1261 gccatcagcc tgaacgaaaa gatgtattcc cagatgctgc gtgtagggga acagctgttc
     1321 gggaactact atattctgcg ggtggatatt cccacgccca gttgtgatcc caagcaggat
     1381 atcgacaagg cgctggaaga agaaatccac aagcagaaag cggcccagca aagcgacgaa
     1441 ttcggcggcc tgttcggcga ttccgcgatc gataccgatg ccctgcgagt gtccctggag
     1501 gggcagcgtc aagcctgcgt gcagcaacac gagatggcaa agaccaacca ggagcgggtc
     1561 accgacagca tccgcgcatt cagggttctg gatcacgcca tgctcgccat cggccacatc
     1621 ggccgcgaca gccagcaact gatcctgctc gccctgatct tcatctgtgg cctcaccgcc
     1681 accatgacca atcaccatat cagcctgcgc ccgttgcaga atgccaatga ctatcgcatc
     1741 agcggcatcg cccagaccat cgccaacctg tctctggcca tatcggcctg ggaatacctt
     1801 cagatcggcg ccaatgccgg cgttcccatg cagaacgaat ccacccgctg gggttatgtg
     1861 atcgtgttct gcctgatgac gggaatcagc ctgctgcagt cgctgttcgt gcccaaatcc
     1921 gccgcaccag gcggcagcat cggcaaggca ctgctgtcga tcccgcttta cgcctatgcc
     1981 acgttcggtg ccgcctacta cttcctgatt gcttccggca acaaacaggg tctggtgctg
     2041 catgtggacc gcatcatgga tgccgccgac ctgttcacca agatcagcct gctgatctgg
     2101 gctggcctgc tgatcaagca gacccacatt ggcgagctgg tattccggat tttcaaaccc
     2161 tggcgcctgc cgccggagtt gctcgccttc gtcgcgattt tcctgatggc ggtacccacc
     2221 gcctacaccg gtgcctccgg cgcaattatc gtcgccatgg gcggcgtggt gtataacgaa
     2281 ctgcgccgcg ccggtgcccg ccgcaacctg gcactggcca ccaccgcaat gaccggcagc
     2341 ctgggcgtgg tgctgaaacc ctgtctgctg gtgttgatca tcgccttcct caacaaggaa
     2401 gtgaccaccg acgatctgtt ccactggggc acccgtgtct tcatcatgac gtccttcatt
     2461 ttcttcgtga ttgcgctgat cgcccgcgag gaaaagctca gcgttgcacc cacccgtgaa
     2521 gcgctggttc ccagcctgaa agccttcatt cccctgttcc cctacgtgat cctggtggca
     2581 attacactgc tgctgttcta cgtgctgctg gatgtgaagc tggatgagaa caccgccgcc
     2641 atcatggtac cgatgatcgt gctggtaatg gtgatctacg agaaagtgat gggcaagaaa
     2701 cgcctggaag agcaagaaac ctatcacgac gaggaacggc agacgacggc ggaaggtgca
     2761 atccgcgtag ctacgtccag caccgccgaa ctgatgggcg gcctgatgct gctgatcgcg
     2821 ctgtcgatgt ccatcggcgg tgtgatcgag gaaacccata tcatccagga cgctgccgaa
     2881 cacggcggac tgttccacaa tcagtggacc gtcatgaccg ccctgatcct ggtgctgatg
     2941 atgatcggca tgagcggcat ggaaccgttc ggcgcggtga tactggtgtc cggctcgctg
     3001 gcgcaggtcg catacaagtt tggcatcgat ccgatcca
//
LOCUS       DASUEG010001020         5044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1495338, whole genome shotgun sequence.
ACCESSION   DASUEG010001020 DASUEG010000000
VERSION     DASUEG010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495338"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..903)
                     /gene="miaB"
                     /locus_tag="VFX11_19110"
                     /pseudo
     CDS             complement(1..903)
                     /gene="miaB"
                     /locus_tag="VFX11_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            1083..3044
                     /locus_tag="VFX11_19115"
     CDS             1083..3044
                     /locus_tag="VFX11_19115"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4940792.1"
                     /translation="MRAGFWRGVAVVLGLTVVAPVMANPDFIAAREAYRNGDNQTVVE
                     KAGVLSRDPLGIYPRYWLLTRQVETVAPEQMRPFLKYYHGAWLAEKLRAEWLVVLGRK
                     GEWALFREQYPLLLDTPSQELRCHAYAARLEAGDDKVLPEARESLWFTAKDLPSACDP
                     VLQALQASPVLTDADRWARLRMALTANAQGLARHLASLLDRSLTAAELQAIASNPARY
                     LDKASLDSRLDRELAVYAIGRLARSDLDGAAARLERREAALGDEPAGFAWQQLGMVAA
                     RKLDGRALDWMARSEGRTSWTDEAIEWRIRMALRAGDWELALRSIRQLSDATRQQRAW
                     QYWEARALEVRKALPGANRIFATLSLDDDYYGLLARDRLGPLVGPSGSVYVVTDEDRK
                     LVRAHAGLQRALALNEMGFRTEAVREWNWSLRNADDRLLLAAAEAANEAGWYDRAIYA
                     ADRTKKLHNYSLRYLAPYREVTRGYALHLGLDESWVYGLIRQESRFVTTARSSVGAGG
                     LMQVMPATAQWVANRIGLKYHAGMVNEVGTNVQLGTYYMKHVLDTLSNQPVLATAGYN
                     AGPNRARAWQPADTPLEAAIYVESIPFLETRDYVKKVMANAVAYSMTFGENRLSLTSR
                     LGVIPPRNAPPSAEEMETEEVATTQADTP"
     gene            3041..3664
                     /locus_tag="VFX11_19120"
     CDS             3041..3664
                     /locus_tag="VFX11_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4940793.1"
                     /translation="MNNIPVARIVAAVVILMALLVAVLAGWRYYQMSRAAQALPINTR
                     MGGDFMLPSTRDVPLDTRQLRGKVVLLNFGFASCPDVCPMVLARLRQVVNELGADASG
                     VQVVFVSFDPARDSIAQLKGYVAHFHPDIIGATGTETEIAAIARRYGVVYMKEDTGSA
                     AGYGFAHSDFIYLLDAEGKVRKLYDNRTLTTDISRDIRTLLAADRLF"
     gene            3642..4178
                     /locus_tag="VFX11_19125"
     CDS             3642..4178
                     /locus_tag="VFX11_19125"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX4940794.1"
                     /translation="MLLTGFSEHNGTPCMKTTNGLFALLVPVLMSLASMSLAAADVAP
                     VQVVAAWVPEPPAVARHAAAYVTLVGGGQDDVLLGATTTAAEIVELHETSLADGVLRM
                     KPVASLPVATRQRIEFTPGGLHMMLINLRQPLRYGSRIPLELRFEKAGKVKVEAEVRS
                     LPPAPASRDAEMHHHHHH"
     gene            4413..>5044
                     /locus_tag="VFX11_19130"
     CDS             4413..>5044
                     /locus_tag="VFX11_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940795.1"
                     /translation="MRHRTFLYSVFSAAVMTTVLSGCGEQKPSPAAGGAGEGMPPVEV
                     GVVTVTPGQLALTSELPGRLEALRTAEVRARVAGTVFERAFREGSDVKAGDLLYRIDP
                     RPYEAQLANARSELARAEAELVEARQRETRARSLIGKSMISQQDFDAAIALAKASEAD
                     VAAKKATVELHRLNVNLASVRAPISGRIGRALVTEGALVGQGEVTPLAVI"
BASE COUNT          899 a         1369 c         1705 g         1071 t
ORIGIN      
        1 ggggcgcacg gccttcagct tgcggatgcg ctcgaggtaa tccgccgtgg tgtggttgcg
       61 cttcatgccg gccagcacgc ggttggcgcc ggactgcaca ggcaggtgca ggtgcgaaac
      121 cagcttgggt gtatcggcgt agacagcgat caggtcgtca ttgaattcca gcggatgcga
      181 cgtggtgtag cggatgcggt cgataccggg aatctcggcg acatggcgca gcagctcggc
      241 aaaggtgcag atgctgccgt catgatgtgg gccccgataa ccgttgacgt tctgaccgag
      301 cagggtgacc tcgcgcacgc ccttgccggc gagcacggcg atttcggcaa tcacgtcatc
      361 gaaggggcgc gagacctcct cgccacgggt atagggcacc acgcagaaag tgcagtactt
      421 cgagcagcct tccatgatgg agacgaaggc cttgaagcct tcgacacgcg gctccggcag
      481 gaagtcgaac ttttcgattt ccgggaagct cacgtcaacg agggtgattt tcctggccag
      541 tgttgatgct tctgcgcgct cgttcagcat ctttggcagg cgatgcaggg tctgtggacc
      601 gaacaccatg tccacaaagg gggcgcgctt ctggatgcct tccccttcct gtgaagctac
      661 acagcccccc acaccgatga cgatgtcggg gcgtgcctct ttcagcttgc gccagcgccc
      721 cagctccgag aacacctttt cctgcgcttt ttcgcggatg gaacaggtgt tgagcaggag
      781 aacatcggct tcttcggggt tggtggtcag ctccatgccg tgggagtcgc ccagcaggtc
      841 ggccatgcgc gagctgtcat actcgttcat ctggcatccc tgggtttcga tataaagctt
      901 cttggccatg gcagcggagg tcaccggtag ggggcaaaaa caggcgcgca ttatagcgat
      961 ggggtgggcc aggggacagc ggcaccggtc agtcctcgtc ggacacaaaa gcagacacaa
     1021 atgccggttg cgcaggggcg ccagccccgt atcctagcca gtcgagatgt gaggagcagg
     1081 gtgtgcgagc tggattctgg cgtggggtgg cggtggttct ggggttgacg gtggtcgctc
     1141 cggtgatggc caaccctgat ttcattgcgg cccgggaggc ctatcgcaac ggcgacaacc
     1201 agacggtggt ggaaaaggcc ggggtattgt cgcgcgaccc gctcggcatc tatccccgtt
     1261 attggctgct gacccggcag gtggagaccg tagcgcctga acagatgcgg cctttcctga
     1321 agtactacca cggtgcctgg ctggcagaaa agctgcgtgc cgaatggctg gtggtgttgg
     1381 ggcgcaaggg tgagtgggcg ttgttccgtg agcagtaccc tttgctgctg gataccccgt
     1441 cgcaggagct gcgctgccat gcgtatgctg ctcgccttga ggcgggagat gacaaggtgt
     1501 tgcccgaggc gcgtgaaagc ctgtggttta cggccaagga tcttccctct gcctgtgatc
     1561 ccgtcctgca ggctttgcag gcatctcccg tgttgacaga tgctgaccgt tgggcgcgct
     1621 tgcgtatggc cctgacggcc aacgcccagg ggttggcacg ccatctggcc tcgctgctgg
     1681 atcgttcact gacagcggcc gagttgcagg caattgcttc caacccggca cgctatctcg
     1741 acaaggccag tctggactcc cggctggacc gtgagctggc ggtctatgcg atcggtcgac
     1801 tggctcgctc ggatcttgat ggggcggctg cccgtctgga gcggcgggag gcggcactgg
     1861 gcgatgaacc tgccggtttt gcctggcagc agttgggcat ggtggcggct cgcaagctgg
     1921 acgggcgcgc cctggactgg atggcgcgca gtgagggccg tacttcctgg accgatgagg
     1981 ccatcgagtg gcgtatccgc atggcactgc gtgccgggga ttgggagctg gcgctgcgca
     2041 gcattcgcca actgagtgat gccacccgcc agcaacgtgc ctggcagtat tgggaggcaa
     2101 gggcgctgga ggtccgcaag gcgttaccgg gcgccaatcg catttttgcc acgctcagcc
     2161 tggatgatga ctattacggt ttgctggcac gtgatcgcct tggccccttg gtcggtccgt
     2221 ctggttcggt ttatgtcgtg acggatgagg atcgcaagct ggtccgggcg catgccggat
     2281 tacagcgtgc cttggcgctc aacgagatgg ggttccggac agaggcggta cgcgagtgga
     2341 actggtcact tcggaatgcc gatgaccgcc tgctgttggc ggccgcagaa gcagccaatg
     2401 aggcaggctg gtatgacagg gcgatttatg cggcagaccg cacgaagaaa ctgcataact
     2461 acagcctgcg ctatctggct ccttatcgtg aggttacacg tggctatgcg ctgcatctcg
     2521 ggttggatga gtcctgggta tacggcctga ttcggcagga aagccgcttt gtgacgacgg
     2581 cgcggtcctc tgtcggggcg ggcggtctca tgcaggtgat gccggcaacg gcacagtggg
     2641 tggccaaccg catcgggctc aagtatcacg cgggcatggt caacgaagtg ggcaccaatg
     2701 tgcagcttgg aacgtattac atgaagcatg tgctggatac gctcagcaac cagcccgtgc
     2761 tggcgacggc gggctacaac gccgggccca atcgtgcgcg ggcctggcag ccggccgata
     2821 caccgctgga ggccgccatc tatgtcgaga gcatcccgtt tctggaaacg cgtgactatg
     2881 tgaaaaaggt catggccaat gccgtggcct acagcatgac gtttggcgaa aaccggctgt
     2941 cgctgacctc gcggctgggt gtgattcctc cgcgcaatgc gccgccatcg gcggaggaga
     3001 tggagacgga agaagtggcg acaacccagg cagatacccc atgaacaata ttccggttgc
     3061 ccgcattgtt gcggctgtgg tcatcctgat ggcgttattg gtggccgtgc tggcgggctg
     3121 gcgctactac cagatgagcc gtgcggcaca ggccctgccg attaacacga gaatgggggg
     3181 cgatttcatg ttgccgtcta ctcgtgatgt cccgctggat acccggcagt tgcgcggcaa
     3241 agtggtgctg ctgaatttcg gctttgcgtc ctgtccggat gtatgcccca tggtgctggc
     3301 tcgtttgcgc caggtcgtca atgagctggg ggccgatgca agcggggtgc aggtcgtgtt
     3361 tgtatcattt gatccggcca gggattccat tgcccagctc aagggatatg tagcgcattt
     3421 ccatcccgac atcattggtg caacaggaac ggaaactgaa attgcagcga ttgcgcggcg
     3481 gtacggggtg gtctacatga aggaggatac cgggtctgcc gccggatacg gatttgcaca
     3541 cagtgatttc atttatttgc tggatgctga gggaaaggtg cgcaagctct atgataatcg
     3601 cacgctgacc accgatatca gtcgtgacat aaggacgctg cttgctgctg accggctttt
     3661 ctgaacataa cgggacacca tgcatgaaaa caacaaacgg attatttgcg ctgctagtgc
     3721 cggtcttgat gtcgttggca tcgatgtcgt tggctgcggc agacgtggcg ccggtgcagg
     3781 tggtcgcggc ctgggtgcct gagccccctg cggtcgcgcg tcatgcggcg gcgtatgtga
     3841 cccttgtggg aggggggcag gatgatgtgt tgctgggggc gaccacgacg gcggccgaga
     3901 ttgtggagct gcatgaaacc agtctggcgg acggcgtgtt gcgcatgaag ccggttgcat
     3961 ctcttccggt ggcgacccgc caacgtatcg agttcactcc cggagggttg cacatgatgc
     4021 tgattaacct gcgtcagccg cttcgttacg gcagtcgcat tccgctggag ttacgttttg
     4081 aaaaggctgg caaggtaaag gtggaggccg aggtgcggtc gttgccgcca gcgccggcca
     4141 gccgagatgc cgagatgcat caccaccatc atcactgagc accttttctt gaaaatcccg
     4201 tacagttgct gcgggatttt ctgtctcttt tttgtttcgg ttttacccct ctccgacagg
     4261 cggctttttt tcaaacagca cgggtgctag gttcccggaa cttgctgctg cgcggttccc
     4321 tttctcctgc tgactgtcat gagaaacgtg cggaactgcc ctaatctgtt agcagctgcc
     4381 attgactccc gatttgttac caggagcgca tcatgcgtca caggaccttc ctgtattccg
     4441 tcttttctgc tgcggtcatg acgaccgttt tgtccggttg tggtgagcag aagccgtcac
     4501 ctgctgcagg tggtgctggt gaaggcatgc cgcctgtcga ggtgggggtg gtgaccgtga
     4561 cgcctggcca actggcgttg acatcggaat tacccggtcg cctggaagcg ctgcggacgg
     4621 ccgaggtgcg tgcgcgcgtg gcggggactg tgtttgaacg ggctttccga gaaggcagtg
     4681 atgtcaaggc aggggatctg ctgtaccgca tcgaccctcg tccgtacgaa gcgcagctgg
     4741 ccaatgcgcg cagcgaactg gcgcgtgccg aggccgaact ggtggaggct cggcaacgtg
     4801 aaacacgtgc ccgctcgctg attggcaaga gcatgatcag tcagcaggat tttgatgcgg
     4861 ccattgcgct ggccaaggca tccgaggccg atgtggcagc caaaaaggcg acggtcgagt
     4921 tgcatcgcct gaacgtgaat ctggcatcgg tgcgtgcgcc catttccggg cgcattggtc
     4981 gtgcactggt aacggagggg gcgttggtcg gtcagggtga agtgacgccg ctggcggtga
     5041 taca
//
LOCUS       DASUEG010001021         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_465062, whole genome shotgun sequence.
ACCESSION   DASUEG010001021 DASUEG010000000
VERSION     DASUEG010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465062"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VFX11_19135"
     CDS             <1..1284
                     /locus_tag="VFX11_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4940796.1"
                     /translation="ATTELERSYRVNLNMIGIDGRPRVKGLYDILNEWLEFRITTVRR
                     RLQYRLDKVLDRLHILEGLLIAYLNIDEVIHIIRTEDEPKQVMMERFGLSDLQTEAIL
                     NLRLRNLAKLEEFQIRGEQEELEAERKTLEEILNSNAKLKKLIVKELQADMDKFGDDR
                     RSPIVQREEAKAMDETELVAAEPVTVVLSKKGWVRAAKGHDVDPVSLSYKAGDEFLAV
                     AKGKSNQNAIFFDSTGRSYTLQAHTLPSARGQGEPLSARLEPPSGATFDGVLMGSDDQ
                     LCLMASDAGYGFIMRLGDAQSRNKSGKALLSLPPKARVLQPKLVQDADNEFVVAVSNE
                     GRMLMFPLRELPELAKGKGNKIIGIPSARAEAREEFVVDVVVINRDQQLKVIAGKRHI
                     GLKFSDLEHYMGERGRRGNMLPRGFQKVDAIEVTE"
     gene            complement(1312..2376)
                     /locus_tag="VFX11_19140"
     CDS             complement(1312..2376)
                     /locus_tag="VFX11_19140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4940797.1"
                     /translation="MKLVIVTDAWHPQINGVVTTMTRVVARARQRGFQVELIDPSRFT
                     TVPCPRYENEIRLAVDVWRIVPMLKQAAPDFVHIVSEGPLGLAARLWLSFNKIPYTSA
                     YHTRFPEYVHNLWPVLSVDLGYRVLRRFHANSRAVFVPTASLYDELAHAGFDHLRLWS
                     RGVDAEHFHPCQRPHGEKLFGHLPQPWFVYVGRVAPEKNLQAFLQLELPGSKIVVGDG
                     PAREALQRQFPASHFTGFQSGVTLAGCFAAADVFVFPSRTDTFGVVLLEAMASGTPVA
                     AFPVTGPKDVVMQGKTGFLSENLAHAALKALQLERQDCRQHATQFTWARAADMLLDHL
                     AWIPAGKAANANHRPLPGLQ"
     gene            complement(2511..>2716)
                     /locus_tag="VFX11_19145"
     CDS             complement(2511..>2716)
                     /locus_tag="VFX11_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HEX4940798.1"
                     /translation="ENADFHVLTKAYRGLPLEAFERFLQLFQDQGRDINARDAKGRTL
                     LQLAQENRSHLDYVEVLKKFGAL"
BASE COUNT          590 a          743 c          878 g          505 t
ORIGIN      
        1 gccaccaccg agctggaacg ttcctatcgc gtcaacctga acatgatcgg catcgatggc
       61 cgcccgcgcg tgaagggtct gtatgacatt ctgaacgaat ggctggaatt ccgcatcacc
      121 accgtgcgcc gccgtctgca ataccgtctg gataaagtgc tcgatcgcct gcatatcctg
      181 gaaggtttgc tgatcgcgta tctgaatatc gacgaagtga tccacatcat ccgcacggaa
      241 gacgaaccca agcaggtgat gatggagcgg ttcggtctct cggatttgca gaccgaagca
      301 atcctgaatt tacgtctgcg caatctggcc aaactggaag aattccagat tcgtggcgag
      361 caggaagaac tggaagctga gcgcaagacg ctggaagaaa ttctcaattc caatgcgaaa
      421 ctgaaaaagc tgatcgtgaa ggaattgcag gccgacatgg acaagtttgg cgatgaccgc
      481 cgctcgccca tcgtgcagcg tgaagaagcc aaagcgatgg atgaaaccga actggtggcg
      541 gcggagccgg tgacggtggt gctgtcgaaa aaaggctggg tgcgcgcggc gaagggccac
      601 gatgtggatc cggtcagcct gagctacaag gccggcgatg aattcctggc ggtggcgaaa
      661 ggcaagtcca accagaacgc gattttcttt gattccactg gccgcagtta cacgctgcag
      721 gcacacacat tgccgtcggc gcgggggcag ggtgaacccc tgtcggcgcg attggaacca
      781 ccttcaggcg ccacgtttga tggcgtgctg atgggcagcg atgaccagct gtgcctgatg
      841 gccagcgatg ccggttatgg cttcatcatg agactgggcg atgcgcagtc gcgcaacaag
      901 agcggcaagg cgttactgag cctgccgcca aaagcccgtg tgctgcagcc gaagctggtg
      961 caggacgcgg acaatgaatt cgtggtggcg gtgagcaatg aaggccgcat gctgatgttc
     1021 ccgctgcggg aattgccgga gctggccaaa ggcaagggca acaagatcat cggcattccg
     1081 tctgcccgtg cggaagcgcg cgaggaattc gtggtggatg tggtggtgat caaccgcgac
     1141 cagcaactga aggtgatcgc cggcaagcgt cacatcggat tgaagttcag cgatctggaa
     1201 cactacatgg gtgagcgtgg gcggcgtggc aacatgctgc cccgtggatt ccagaaagtg
     1261 gatgcgatcg aggtgacaga gtaagttcag tgcaggctgc aggggacccg gtcactgcag
     1321 ccctggcagg ggccggtggt tggcgttggc cgccttgcct gccggaatcc acgccagatg
     1381 gtccagtagc atgtccgccg cccgcgccca ggtgaactgg gtggcgtgct ggcgacagtc
     1441 ttgtcgttca agttgcagcg ctttcaaggc cgcgtgcgcc agattttccg acagaaatcc
     1501 ggttttcccc tgcatcacca catctttcgg acccgtcact ggaaatgctg cgacgggtgt
     1561 gccgctggcc atcgcttcca gtagcactac accaaaggtg tcggtacggg acgggaacac
     1621 gaacacgtcg gcggccgcga agcagcccgc cagggtgacg ccggactgga agccggtgaa
     1681 atgcgaagcg ggaaattgcc gttgcagtgc ttcccgcgcc gggccgtcac cgacgacgat
     1741 tttggaaccc ggcagctcca gttgcagaaa cgcctgcaga tttttttccg gtgccacacg
     1801 gcccacgtag acaaaccagg gctgcggcag gtggccaaac agcttttcac catgagggcg
     1861 ctggcaggga tggaaatgct cggcgtccac gccccgcgac cacagacgca ggtgatcaaa
     1921 accggcgtgt gccagttcat cgtaaaggct ggcggtgggc acgaacacgg cgcggctgtt
     1981 ggcgtgaaag cggcgcagta cgcgatagcc gagatcaacg ctgagcacgg gccaaaggtt
     2041 gtgcacatat tcgggaaaac gggtgtggta ggcggaggtg tagggaattt tattgaagct
     2101 gagccatagg cgtgcagcca gccccagcgg accttccgag acaatgtgaa caaaatccgg
     2161 cgccgcctgc ttcagcatgg gcacgatgcg ccatacatct actgccagcc ggatttcatt
     2221 ttcgtaacgg gggcagggca ccgtggtgaa gcgggaagga tcaatcagtt ccacctggaa
     2281 tccccgttgc cgggcccgcg ccaccacccg cgtcatggtg gtgacgacac cgttgatctg
     2341 cgggtgccag gcgtccgtca cgatgaccag tttcattctg cgactccttg ccgcgcacag
     2401 cgcaggggag ccgggcgcat gttgtcatac atgcgcgaat tgtggtcagg tcaggttaaa
     2461 accgggtgac agggaacgga acttcagatg acggaacgtt gtaggaatga ttacagcgcc
     2521 ccgaacttct tcaacacctc gacgtagtcc aggtggctgc ggttttcctg cgccagttgc
     2581 agcagggtgc ggcctttggc atcgcgggca ttgatgtcgc ggccctggtc ctggaacagc
     2641 tgcaggaaac gttcgaatgc ctccagcggc agaccgcgat aggccttggt caggacatga
     2701 aaatcggcgt tctcgt
//
LOCUS       DASUEG010001022         7862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_921209, whole genome shotgun sequence.
ACCESSION   DASUEG010001022 DASUEG010000000
VERSION     DASUEG010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921209"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="VFX11_19150"
     CDS             complement(<1..1707)
                     /locus_tag="VFX11_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX4940799.1"
                     /translation="MMNPLLASHRLPPFSQIKPEHVVPAIDTILTENRQQIEAIVAHV
                     QQHDVSVENFLLPFEELNDRLSNAWSPVGHLNGVLNSEALRNAYNSCLPKLSEYGTEL
                     GQHKGLFQAYTELKNSPQFAQLTQAQKKVVDNALRDFHLSGIDLPPEQQKRYGEISKR
                     LSELTSKFSENVLDCTNAFSKHVMDVTLLEGLPETQLQAARIAAQKKNLEGWLLTLDI
                     PVYIAVMTYCDNRTLRREFYEAYNTKASDQGPFAGKFDNNPLMQEILALRHELANLLG
                     FANYAERSIATKMTETTDQVMEFLQDLARKALPAAKQEIRELTEFAVDYCNADDLQAW
                     DVSYYSEKLKQQRFAISQEALRPWFPLEKVLAGLFEIARRLFDVTFQLDESVDRYHPD
                     VRFYRVFEADEEIAGFYFDLFARANKRGGAWMDECRVRRRTLNGSLQLPVAYLTCNFN
                     GPVGDKPALLTHSEVTTLFHEFGHGLHHMLTKVDDGAVSGINGVAWDAVELPSQFLEN
                     WCWEKEAIPLMSGHYETGEPLPQDMLDQMLAAKNFQSAMMIVRQLEFSIFDFRIHRDY
                     KPG"
     gene            complement(1830..2342)
                     /locus_tag="VFX11_19155"
     CDS             complement(1830..2342)
                     /locus_tag="VFX11_19155"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX4940800.1"
                     /translation="MKSSSRILASLLLASGLAFATTSMAEHRHDHEQTKQCPGKSGMG
                     MMGGGMMGGGMMGKGMHGGMGDMDHKPPFLRGIDLTEEQRDKIFEITYAQIPVHRTFR
                     KEMHKLQQQMHELPLSADYDAGKMKKLVDAQTKLQGEHMLKMAEAHNKIYKLLTDEQR
                     KELAEHHKKD"
     gene            2472..2684
                     /locus_tag="VFX11_19160"
     CDS             2472..2684
                     /locus_tag="VFX11_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940801.1"
                     /translation="MEGDAAGGLIEIKGARYGQKNPAGWESCGVQLNTIITGTRLALR
                     RLPENATNCRRAANTAVTFKIQPATP"
     gene            complement(2659..3480)
                     /gene="aroE"
                     /locus_tag="VFX11_19165"
     CDS             complement(2659..3480)
                     /gene="aroE"
                     /locus_tag="VFX11_19165"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX4940802.1"
                     /translation="MSIDRYAVMGNPIKHSKSPQIHKAFALQTDQDMTYTALLVPEEG
                     FREAVDNFRAEGGKGINVTVPFKLEAFDLVNKRSERAERAKAVNTIVVRTDGSLFGDN
                     TDGVGMVRDITHNHDGRFTGKKVLVVGAGGAVRGVLAPLLKENPAQVIITNRTFEKAR
                     ELAQEFQDLGRIDAVAMNRLEGPFNWIINGTSASLGGSVPELPASVVGPHTWVYDMMY
                     GKDATPFNAWALQHGAEKAFDGLGMLVEQAAESFRLWRGVMPSTAPVIKELRAGF"
     gene            complement(3477..4394)
                     /gene="hemF"
                     /locus_tag="VFX11_19170"
     CDS             complement(3477..4394)
                     /gene="hemF"
                     /locus_tag="VFX11_19170"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX4940803.1"
                     /translation="MTTIDLDAVKRYLLALQDDICRQLAEEETDGQTFLEDSWTREAG
                     GGGRSRVLEGGTVFEKAGVNFSHVYGDGLPKSATAHRPELEGRRFQAMGVSLVIHPRN
                     PLVPTSHANVRFFIAEKDGADPVWWFGGGFDLTPYYGFEEDCVHWHQTAKAACDPFGA
                     DVYPRYKKWCDEYFFLKHRNETRGVGGLFFDDLNQWEYEKCFAFMRSVGDAFIKAYRP
                     LVQKRKHLPYTEPQREFQLYRRGRYVEFNLVYDRGTLFGLQTGGRTESILMSLPALVS
                     WKYNWHPEPGTAEARLYTDFLGARQWIEA"
     gene            complement(4420..4983)
                     /locus_tag="VFX11_19175"
     CDS             complement(4420..4983)
                     /locus_tag="VFX11_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX4940804.1"
                     /translation="MLSLRHRLRAVHTLATGGVIAYPTEAVWGLGCDPFNAAAVERLL
                     ALKRRPAHKGLILVAADQEQIAPLLEGLTDPQREQLNRTWPGPVTWLISDLHNRIPAN
                     VKGNSTSVAVRVSAHPLVRELCRAWGGPLVSTSANRTGQDPFRSEWALRRHGLLQPDL
                     ILPGRTQQLNKPTEIRDLSTGRIIRAG"
     gene            complement(4987..5496)
                     /gene="purE"
                     /locus_tag="VFX11_19180"
     CDS             complement(4987..5496)
                     /gene="purE"
                     /locus_tag="VFX11_19180"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX4940805.1"
                     /translation="MSQPFVAILMGSDSDFPQVEPAVDTLRKLGIAVEVKVTSAHRTP
                     EITHAYVKDADQRGCSVFICAAGMAAHLAGAVSANTVKPVIGIPIDVTLNGLDALLST
                     VMMPGGIPVATVAIGKAGAKNAGYLAAQMMALADPALAQKLKADRAANAQQVQEKDAA
                     LQARLAAKG"
     gene            5725..6669
                     /locus_tag="VFX11_19185"
     CDS             5725..6669
                     /locus_tag="VFX11_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX4940806.1"
                     /translation="MIKHLPLLAAALLAAGCSTVTQQATNTLPVTQPQISPTLQQPEF
                     KGLKRKVAIARFSNETSAGASFLLDASNDRIGKQASDILSARLVETNKFILLERADLD
                     KIQNEAALANLSKVNVGADYLIIGSVSEYGREAVSETGIFSRNKKQLARAKVNVRLVD
                     VRNGRVIYSEEGSGEALSEANKVFGVGESAGYDTSLDDKAISSAISKLVSNLVENLMD
                     SPWQAYLIGQQDGFFIMTGGKSQGVKPGDQFSVLRKGKVVRNPQTGLDLELPGTPVAK
                     LQVASQAGQGDNEISLCQAVEGSLQGLDLTTLVVQEIR"
     gene            6686..7000
                     /locus_tag="VFX11_19190"
     CDS             6686..7000
                     /locus_tag="VFX11_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940807.1"
                     /translation="MKKIIVGLLALLLAACGSFKTQVQTEEGSSLHLVGNPEQAVLVL
                     DNTTTLDLNTTKSFDLNGKQVTKIVVPKGQHRVTITRNGALLVDRSIYVGEGQAFEIV
                     LP"
     gene            7032..7388
                     /locus_tag="VFX11_19195"
     CDS             7032..7388
                     /locus_tag="VFX11_19195"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEX4940808.1"
                     /translation="MQKKLLISLSLSAVLLTGCVVAPPHATGFYWGNYSRTLYEYQKT
                     PSPSTLEAHRNSLLDIIARADSGQSKVPPGIYAELGKLHLDKGEKAEAVKWFQQEVAH
                     YPESGVLMKTLIEKAG"
     gene            7402..>7862
                     /locus_tag="VFX11_19200"
     CDS             7402..>7862
                     /locus_tag="VFX11_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HEX4940809.1"
                     /translation="MSRISMLRALAPAALLLGVSLLPGCATAPATAPITNQEVFTGIY
                     SEKPNSILVLPAINHSTAADAPLYYIASIAQPLTEQGYYVLPVPVTNALLQQAGISDG
                     AQLLAVPPQRFGQMFGADAILFVTIDSWDTSYYVLGGNVTVALTFKLVSTR"
BASE COUNT         1492 a         2413 c         2396 g         1561 t
ORIGIN      
        1 gcccggcttg taatcgcggt gaatgcggaa atcgaaaatg gaaaattcca gctggcgcac
       61 gatcatcatc gccgactgga aatttttcgc cgccagcatc tggtcgagca tgtcctgcgg
      121 cagcggttcg ccggtctcgt aatggcccga catcagtgga atcgcttctt tttcccagca
      181 ccagttttcc aggaactggc tgggcagctc caccgcatcc caggccacgc cgttgatgcc
      241 ggacaccgcg ccgtcatcca ccttggtcag catgtgatgc aggccgtggc cgaattcatg
      301 gaacagggtg gtgacttcac tgtgcgtgag cagggcaggt ttgtcgccga ccgggccgtt
      361 gaaattgcag gtcaggtacg ccaccggcaa ttgcaggctg ccattcaacg tgcgtcggcg
      421 cacgcggcat tcgtccatcc aggcgccgcc gcgcttgttg gcgcgggcga acaggtcgaa
      481 atagaaaccg gcaatttcct cgtccgcctc gaacacgcga tagaaacgca cgtcgggatg
      541 atagcgatca acggattcat ccagctggaa ggtcacgtca aacaggcgac gcgcaatttc
      601 aaacagccct gccagcactt tttccagcgg aaaccagggc cgcagtgctt cctgcgaaat
      661 cgcgaaacgt tgctgcttga gtttttcgct gtagtaggac acatcccacg cctgcagatc
      721 gtcggcgttg cagtagtcca cggcgaattc ggtcagctcg cggatttcct gtttggccgc
      781 cggcagtgcc ttgcgcgcca gatcctgcag gaattccatc acctgatcgg tggtctcggt
      841 catcttggtg gcgatggacc gttcggcgta attggcaaag cccagcaggt tggccagctc
      901 gtggcgcagc gccagaattt cctgcatcag cggattgttg tcgaacttgc cggcgaacgg
      961 gccctggtcc gacgccttgg tgttgtaggc ttcgtaaaat tcgcggcgca gggtgcggtt
     1021 atcgcagtag gtcatcactg cgatatagac gggaatatcc agcgtcagca gccagccttc
     1081 cagattcttt ttctgcgcgg cgatccgggc agcctgcagc tgggtttccg gcaggccttc
     1141 cagcaacgtc acatccatca cgtgtttgct gaaagcattg gtacagtcga gcacgttttc
     1201 agaaaacttg ctggtgagtt ccgacaggcg cttgctgatc tcgccgtaac gtttctgctg
     1261 ctccggcggc agatcgatgc cggacaggtg gaaatcccgc agcgcattgt ccaccacctt
     1321 tttctgcgcc tgggtcagtt gcgcaaactg cggcgaattc ttcagctccg tgtacgcctg
     1381 gaacagcccc ttgtgctggc ccagctcggt gccgtattcc gacagcttcg gcaggcagct
     1441 gttgtaggca ttgcgcaggg cctcgctgtt cagcacccca ttgaggtgtc ccaccggcga
     1501 ccaggcattg ctcaggcggt cattcagttc ctcgaacggc agcaggaagt tctccaccga
     1561 cacatcgtgc tgctgcacat gggcgacgat ggcttcgatc tgctggcggt tttcagtgag
     1621 gatggtgtcg atggcgggaa caacgtgctc gggcttgatc tggctgaacg ggggcaggcg
     1681 atgggaggca agcagtggat tcatgatgac cggggacctg atagagggac atgggcctag
     1741 tgtaaccacc ctgtgcctga tcaccaaggg ctgcccgcca gaccacggcc ggtctggcgg
     1801 ggtgcctgag attgcccagg gggaagcggt cagtccttct tgtggtgctc cgccagctcc
     1861 ttgcgctgtt cgtcggtcaa cagcttgtag atcttgttgt gggcctcggc cattttcagc
     1921 atgtgctcgc cctgcagctt ggtctgggcg tccaccagct ttttcatttt gccggcatca
     1981 taatccgccg acaggggcag ttcatgcatc tgctgctgca gcttgtgcat ttccttgcgg
     2041 aatgtgcggt gaacggggat ctgggcgtag gtgatctcaa aaatcttgtc gcgctgttcc
     2101 tccgtcaggt cgatgccgcg caggaacggt ggcttgtgat ccatgtcgcc catgccgccg
     2161 tgcatgcctt tgcccatcat gccgccaccc atcattccac cgcccatcat gcccatgccg
     2221 gatttgcccg gacactgctt ggtttgctcg tggtcgtggc ggtgttccgc catggaggtg
     2281 gtggcgaaag ccaggccgct ggccagcagc agcgaggcga ggattcggct ggatgatttc
     2341 atgaggaagt ctcctgaaga taagtctgtt gccgtttgat ttgcgccggg atgaacagtt
     2401 cccaatccgg cgacctggaa cggagtatga cagtgctttg tgcaagttcc ggtgagaaat
     2461 gtggaaacag tatggaaggt gacgccgccg gaggattgat tgaaatcaag ggtgcccgtt
     2521 atggacaaaa aaaccccgca gggtgggagt cctgcggggt tcaactcaac acaattatta
     2581 ccggcaccag attagcgctg cgtcgcctgc ctgaaaatgc gaccaattgt cgcagggcgg
     2641 cgaacacggc tgtcacattc aaaatccagc ccgcaactcc ttgattaccg gagcggtact
     2701 gggcattacg ccgcgccaca ggcggaacga ttccgccgcc tgctccacca gcatgcccag
     2761 accatcgaat gctttttccg caccgtgctg cagcgcccag gcgttgaacg gcgtggcgtc
     2821 cttgccgtac atcatgtcgt acacccaggt gtgggggccg accaccgacg ccggcagttc
     2881 cggcacgctg ccgcccaggc tggcagaggt gccgttgatg atccagttga acggcccttc
     2941 cagccggttc atggcaaccg cgtcgatgcg gcccagatcc tggaattcct gcgccagctc
     3001 ccgcgctttt tcaaaggtgc ggttggtgat gatcacctgg gcgggatttt ccttcagcag
     3061 cggtgccagc acaccgcgca ctgcgcctcc ggcacccacc accagcactt tcttgccggt
     3121 gaaacggccg tcatggttat gggtgatgtc gcgcaccatg cccacgccat cggtattgtc
     3181 gccaaacagc gagccgtcag tgcgcaccac aatcgtgttc acggctttcg cccgctccgc
     3241 ccgctcgctg cgcttgttca ccagatcaaa ggcttccagc ttgaatggca ccgtaacgtt
     3301 gatgcctttg ccgccctcgg cgcggaaatt gtccactgcc tcgcggaaac cctcttcggg
     3361 caccagcaag gcggtgtagg tcatgtcctg atccgtctgc agcgcgaacg ccttgtggat
     3421 ctgcggtgat ttggaatgct tgatgggatt gcccatcacg gcgtagcgat caatgctcat
     3481 gcttctatcc attggcgggc accgagaaaa tccgtgtaca ggcgcgcttc cgccgtgccc
     3541 ggttccgggt gccagttgta tttccagctc accagcgccg gcagcgacat caggatcgat
     3601 tcggtgcggc cgccggtctg caaaccaaac aaagtgccgc gatcatagac caggttgaat
     3661 tccacgtaac ggccccgacg gtagagctgg aattcgcgct gtggttccgt gtacggcaga
     3721 tgcttgcgct tctgcaccag cggacggtag gccttgatga aggcgtcacc cacgctgcgc
     3781 atgaaggcaa aacatttctc gtattcccac tggttgaggt cgtcgaaaaa caggccgccg
     3841 acaccgcggg tttcgttgcg gtgtttcagg aagaagtatt cgtcgcacca ttttttgtag
     3901 cgcggataca catcggcgcc gaagggatcg cacgcggctt tggcggtctg gtgccagtgc
     3961 acgcagtcct cttcgaaacc gtaatagggg gtcaggtcga agccgccgcc gaaccaccac
     4021 accgggtcgg caccgtcttt ctcggcgatg aagaaacgca cgttggcgtg ggaggtcggc
     4081 accagcgggt tgcgcggatg gatcaccagc gacaccccca tggcctggaa ccggcgccct
     4141 tccagttcgg gccggtgggc agtggcggac ttgggcaggc catcgccata cacatgggaa
     4201 aagttcaccc cggccttctc gaatacggtg cccccttcga gcacgcggga gcgaccaccg
     4261 ccgccggcct cgcgggtcca gctgtcttca aggaaggtct gtccgtctgt ttcttcttcg
     4321 gccagctggc ggcagatatc atcctgcaag gccagcaggt agcgtttcac cgcgtccaga
     4381 tcgatggtcg tcatacgtct tcccccgtgc tccatggcat caaccggcgc ggattatacg
     4441 cccggtggac aaatcccgta tttcagtggg cttatttaat tgttgggtgc gccccggcag
     4501 gatcaggtcc ggctgcagca ggccgtggcg gcgcagggcc cattcgctgc gaaaggggtc
     4561 ctggccggtg cggttggcac tggtggatac cagcggtcca ccccaggccc ggcacagttc
     4621 ccgcaccagt gggtgagcgc ttacccgcac tgcaacactg gtggaattgc cctttacgtt
     4681 ggcaggaatc cggttgtgca gatcggaaat cagccaggtg accgggccgg gccaggtgcg
     4741 gttcagctgc tcgcgctgcg gatcggtcag accttccagc aggggtgcaa tctgctcctg
     4801 gtccgccgcc accaggatca ggcccttgtg ggccggccgc cgcttgagtg ccagcaggcg
     4861 ctcgacggcg gcggcattga acggatcaca ccccagcccc cacactgctt cggtgggata
     4921 ggcgatcacg ccgccagtgg ccagggtgtg cacggcacgc aaccggtgcc gcagggacag
     4981 catggttcag cctttggccg ccagacgggc ctgcagggcc gcgtcctttt cctgcacctg
     5041 ttgggcgttg gcagcacggt cggccttcag cttctgggcc agggcgggat cggccagggc
     5101 catcatctgc gccgccagat agcccgcgtt cttggcgccg gccttgccga ttgccacggt
     5161 ggcgacggga atgccgcccg gcatcatcac ggtggacagc agggcatcca ggccgttcag
     5221 ggtgacatcg atcggaattc cgatcaccgg cttcacggta ttggccgata cggcgcctgc
     5281 cagatgcgct gccatgcccg ccgcgcagat gaacaccgag caaccgcgct ggtcggcatc
     5341 cttcacgtag gcatgggtga tctcgggcgt acgatgggcg gacgttactt tcacttccac
     5401 ggcaataccc agcttgcgca gggtgtccac ggcgggttct acctggggga agtcggaatc
     5461 ggaacccatc aggatggcga cgaagggctg gctcatgatg cacctttagg ttgacgtgaa
     5521 aaatggggta gcaggcgttc gaggcgggca agttacccca gtccccgggg ccttgcaagt
     5581 ggcgtaaaca gccgtaaaaa gcgtgtggtc aagtgatttc gtacggtaat tttgccattt
     5641 ccgcctacat tgaccctgtc agtgccgctg cctagaatcc ccgggcaccg ttctcatatc
     5701 ttcttctgtt ttacaggaac gtccatgatc aagcatcttc ccctgctggc agccgcgctg
     5761 ctggcggccg gttgctccac cgtcacccag caggccacca ataccctgcc ggtcacccag
     5821 ccccagatca gccccaccct gcagcagccc gagttcaagg gcctcaagcg caaggtcgcc
     5881 atcgcccgtt tcagcaatga aaccagcgcc ggcgccagct tcctgctcga cgccagcaat
     5941 gaccgcatcg gcaagcaggc cagcgacatc ctgtcggcgc ggctggtgga aaccaacaaa
     6001 ttcatcctgc tggagcgggc cgacctggac aagatccaga acgaagccgc tctggccaac
     6061 ctgagcaagg tgaacgtcgg tgccgactac ctgatcatcg gttcggtgtc ggaatacggc
     6121 cgcgaggcgg tgagcgaaac cggcattttc agccgcaaca agaaacagct ggcgcgggcg
     6181 aaagtgaacg tgcggctggt ggacgtgcgc aacggccgcg tcatctactc ggaagaaggt
     6241 tccggtgaag ccctgtcgga agccaacaag gtgttcggcg tgggcgaaag cgccggctac
     6301 gacaccagcc tggacgacaa ggccatttcc tccgccattt ccaagctggt gagcaatctg
     6361 gtggaaaacc tgatggactc accctggcag gcctacctga tcggccagca ggacggtttc
     6421 ttcatcatga ccggcggcaa gtcccagggc gtgaagccgg gtgaccagtt cagcgtgctg
     6481 cgcaagggca aggtggtgcg caacccgcag accggcctcg acctggaatt gccgggcaca
     6541 ccggtagcca aactgcaggt ggccagccag gccgggcagg gcgacaacga aatctccctg
     6601 tgccaggccg tcgagggctc actgcagggc ctggatctga caaccctggt ggtccaggaa
     6661 atccgctgaa ttcgaggaca acaacatgaa aaagatcatc gtgggcctgc tggccctgct
     6721 gctggccgcg tgtggcagct tcaagaccca ggtgcagacc gaggaaggca gcagcctgca
     6781 tctggtcggc aatccggagc aggccgtgct ggtgctggac aacaccacca ccctggatct
     6841 gaacacgacc aaatcgttcg acctgaacgg caagcaggtc accaagatcg ttgtgccgaa
     6901 aggccagcac cgggtgacca ttacccgtaa cggcgccctg ctggtggacc gctccattta
     6961 cgtcggcgaa ggccaggcct tcgaaatcgt cctgccctga tactttggcc tgaacctacc
     7021 ggaagtagaa catgcagaag aagttgttga tcagcctgag cctgagcgcc gtcctgttga
     7081 cgggctgcgt cgtggcgccg ccccatgcca ccggattcta ctggggtaat tattcccgca
     7141 ccctgtacga ataccagaag acaccctcgc cctcgactct ggaggcccac cgcaacagcc
     7201 tgctggacat catcgcccgg gccgacagcg gtcagtcgaa agtaccgccg ggcatctatg
     7261 ccgaactggg caagctgcac ctggacaagg gcgagaaagc agaagcggtg aaatggttcc
     7321 agcaggaagt ggctcattac ccggagtccg gcgtgctgat gaaaaccctg attgagaagg
     7381 cgggctgaga ccatgcgcag aatgagcaga atttcgatgt tgcgtgcgct ggcaccggcg
     7441 gccctgctgc tgggcgtgag cctgttgccc ggctgcgcca ccgcgccggc cacggcgccc
     7501 atcaccaacc aggaagtgtt cacgggcatt tattcggaaa agcccaattc catcctggtg
     7561 ctgccggcca tcaatcactc caccgccgcc gatgcgccgc tgtattacat cgccagcatc
     7621 gcgcaaccgc tcaccgagca gggctactac gtgctgccgg ttccggtgac caacgcgttg
     7681 ctgcaacagg ccgggatcag tgacggtgcc cagttgctgg cggtgccgcc gcagcgtttc
     7741 ggccagatgt tcggcgccga cgccatcctg ttcgtcacca tcgattcctg ggacacctcc
     7801 tactacgtcc tgggcggcaa tgtgaccgtg gcactcacct tcaagctggt gtccacccgc
     7861 ag
//
LOCUS       DASUEG010001023         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_15970, whole genome shotgun sequence.
ACCESSION   DASUEG010001023 DASUEG010000000
VERSION     DASUEG010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15970"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFX11_19205"
     CDS             complement(<1..628)
                     /locus_tag="VFX11_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4940810.1"
                     /translation="MTQSLDFEGIERLPLKEYTERAYLDYSMYVILDRALPHIGDGLK
                     PVQRRIVYAMSELGLKSTAKHKKSARTVGDVLGKFHPHGDSACYEAMVLMAQSFSYRY
                     PLIDGQGNWGSPDDPKSFAAMRYTESRLAPYADVLLAELEQGTVEWVPNFDGTLEEPS
                     LLPSRLPNVLLNGATGIAVGMATDIPPHNLREVAQATVHLIKHPNTSLD"
     gene            complement(791..1375)
                     /locus_tag="VFX11_19210"
     CDS             complement(791..1375)
                     /locus_tag="VFX11_19210"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4940811.1"
                     /translation="MKKTLLASALLLAAAPAFAGNFNYTYIEGGYGEVDASYNDFDVD
                     GDALFLGGSVDLQKGFGIIGSFYTIDFDEDIDGTIFTVGGQFHTPISSQADFVGSIQL
                     INAEVEYDGRYGYDDSADDTGLLLRGGIRFAIQQNLQLEGDISYIDNDFWDDDELGLK
                     AGLRFFFNRQFSLAGGFASDQELDGLYISGRFDL"
     gene            complement(1484..3538)
                     /locus_tag="VFX11_19215"
     CDS             complement(1484..3538)
                     /locus_tag="VFX11_19215"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX4940812.1"
                     /translation="MRTLLKRLGYGLLLLVLLALGGGIFTVWPFVMFPVTEIRIQRTA
                     PLPVIPGETLLAGVAERDITPPVGIPKMGYSAWAREADGFRNRLKTRAFYLKPKQGEP
                     IVVLQADLPASSLVLHHKVADLIAARTDVAMHNLSLHVTHTHSGPGQFMDSDFYNTFG
                     SNKPGFDPALFDFLATQMADAVMAAYESRRPAKIAIGSTAIYGATKNRAMGAYVRNEN
                     VTDKSEDAAAALRAVNPLITLVRIDALTDAGDYRPLGAFSSFAIHGTGIPPFTHPYHG
                     DVWTFFERELEWNIARQYQTPWQPVHGPFEANHADNNPNYRHGLRGDSETRRIGLALA
                     DRAWQLFQSLDTQLQDDVQVSSAMREIDLLNLRPQDEKMLCERAIAGTAVVGAAQGDE
                     VFPISYLPPFQRGWPDEAGDDCHAEKRWMLSKLQAWGLAPTRYPHRVSFTLFRIGDLA
                     LLGLPFEITFEAGNRIASAVTDAFGVAANPVSRVVVSSHTNGFFGYSATREEYSAQWY
                     EGGHTIYGPNTTEFLAKESAKLAGELLRQPGYADLPQEWTFALASKSYYPAPVTPSGH
                     RVELQVPRFVDAQANQEPYWEMQLLDVHPSAIALHEPLLAIEFSDDGEHFVPLLDGQG
                     VAVNDQGYDLQIIHDGASDAGMARYFLRWHNPLIASPGRWYRFRVEARAGQEVFHSSQ
                     FR"
     gene            complement(3626..4153)
                     /locus_tag="VFX11_19220"
     CDS             complement(3626..4153)
                     /locus_tag="VFX11_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940813.1"
                     /translation="MSSSPKFKIIVNGVAEGYHPERVRAAVGNRFGITPAQVEQLLAS
                     QGGLLTELVDHQTAWQLKNLLQQLGVNCRIAPVPLSGLSERTQNMSLHSPAAGRHIGH
                     APVRRANRQSVPMRSGKASIRPTSRVQTSSHTPIWRLVAVVGVALVLGWYLQSGRPPF
                     QHGAAPAPVVADVVR"
     gene            4547..>4990
                     /gene="fabA"
                     /locus_tag="VFX11_19225"
     CDS             4547..>4990
                     /gene="fabA"
                     /locus_tag="VFX11_19225"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HEX4940814.1"
                     /translation="MLKKAFYSRPELENFLHANETEGPQLPQGNLRMMDRVVSISETE
                     GRYQRGALVAEYDLDPQQWFFSCHFPGDPVMPGCLVLDGLWQCLGFYLAWRGYHGKGR
                     AIGVDTVRFFNEIRPQSGSVQYRLHVRRILTRDFTLGVADGEVWVG"
BASE COUNT         1042 a         1476 c         1553 g          919 t
ORIGIN      
        1 tgtccaggct ggtattgggg tgcttgatca agtgcaccgt ggcctgtgca acttcgcgca
       61 ggttgtgcgg cggaatgtcg gtggccatgc ccaccgcaat gccggtggcg ccgttcagca
      121 acacattggg cagacgcgac ggcagcagcg acggttcttc cagcgtgccg tcaaagttcg
      181 gcacccattc caccgtgccc tgctccagtt ccgccagcag cacgtcggcg taaggtgcca
      241 gccgcgattc ggtgtaacgc atggcggcga aggatttggg atcgtccggc gaaccccagt
      301 taccctgacc atcaatcagc ggatagcggt acgagaatga ctgcgccatc agcaccatgg
      361 cttcgtagca ggcgctgtcg ccgtggggat ggaattttcc gagaacatca cccacggtgc
      421 gggcggattt cttgtgcttg gcggtggatt tcaggcccag ctcggacatg gcgtacacga
      481 tgcggcgctg caccggcttg aggccgtcgc cgatatgcgg cagcgcgcgg tcgaggatga
      541 cgtacatgga gtaatccagg taggcacgct cggtatattc tttcaacgga aggcgttcga
      601 tgccttcgaa atcgagggat tgggtcatgg tggctcggtg acagggacgg gtagaatgaa
      661 caagttgctg ttcattctaa tagaagaaac cgaaaaatac agaaccggct cagagggttg
      721 ggccgcaggg gaagaaaaac ggcaggtgga agcaatattg ctgctcccgc ccgccgccag
      781 gtcagggcgc ttacaggtcg aagcggccgc tgatatacag accatccagt tcctggtcgg
      841 aagcaaagcc tccggcaagg gagaactggc ggttgaagaa gaaacgcagg ccggctttca
      901 ggcccagctc gtcgtcatcc cagaaatcgt tgtcgatata ggagatgtcg ccttccagct
      961 gcaggttctg ctggatggcg aaacggatgc cgccgcgcag caacaggccg gtgtcatccg
     1021 cactgtcgtc gtaaccgtag cggccgtcat actccacctc ggcattgatc agctggatgc
     1081 tgcccacaaa atcggcttgg ctgctgatcg gcgtatggaa ctggccaccc acggtaaaaa
     1141 tggtgccatc aatgtcttca tcgaagtcga tagtgtagaa gctgccgata atgccgaagc
     1201 ccttctgcag atccaccgaa ccgcctagga acagggcgtc gccgtccacg tcaaaatcgt
     1261 tgtaagaagc gtccacttcc ccgtaaccac cttcgatgta ggtgtaattg aaattgccgg
     1321 cgaatgccgg tgcagccgcc aacagcaagg cgctggcaag cagggtcttc ttcatgattg
     1381 ctctcctgtg aggtcaaagt ggcgaaatgg taaccccccg cgccgtcaca acccaagccc
     1441 gaccgccgga tttgtgcatc ggatcaccaa aaacggcgcg gcatcaacgg aactgcgagg
     1501 aatggaatac ttcctgaccg gcgcgggcct cgacacgaaa gcgataccag cggccgggag
     1561 aagctatcag gggattgtgc cagcgcagga aataacgggc catgccggca tcgctggcgc
     1621 catcgtggat gatctgtaaa tcgtaaccct ggtcgttcac cgccacaccc tgcccgtcca
     1681 gcagcggcac aaagtgttcg ccgtcgtcgc tgaactcgat ggccagcagg ggctcgtgca
     1741 gggcgatggc cgacggatgc acgtccagca gctgcatttc ccagtagggt tcctgattgg
     1801 cctgggcatc cacgaaccgg ggcacctgca gctccacccg atggccgctg ggcgtcaccg
     1861 gggccggata atagcttttg ctggccaggg cgaaggtcca ttcctgcggc aggtcggcat
     1921 aaccgggttg ccgcaacagc tctcccgcca gcttggcaga ttccttcgcc agaaattcag
     1981 tggtgttggg gccgtagatg gtgtggccgc cttcgtacca ctgggcggaa tattcctcgc
     2041 gcgtggcgct gtagccgaag aagccgttgg tgtgactgct gaccaccacg cgggaaacgg
     2101 gatttgccgc aacgccaaac gcatcggtca cagcggacgc aatgcggttg cccgcttcaa
     2161 acgtgatttc gaacggcagg cccaacagag ccagatcgcc aatgcggaac agtgtgaacg
     2221 acacccggtg cggatagcgg gtcggcgcca gcccccatgc ctgcagcttc gatagcatcc
     2281 agcgtttttc cgcatggcag tcatcgccag cctcatccgg ccagccgcgc tggaacggtg
     2341 gcaggtaact gatgggaaat acttcgtcgc cctgcgccgc acccaccacg gcggtgccgg
     2401 caatcgcgcg ctcgcacagc attttttcat cctgcgggcg caggttcagc agatcgattt
     2461 cgcgcatggc gctgctgacc tgcacgtcgt cctgcaattg cgtgtcgagg gactggaaca
     2521 gttgccacgc cctgtccgcc agcgccagac cgatgcgacg ggtctcactg tcaccccgca
     2581 ggccatgacg atagttggga ttgttgtcgg cgtgattggc ttcaaatgga ccatgtaccg
     2641 gctgccacgg ggtttgatac tgacgcgcga tattccattc cagttcgcgc tcgaagaagg
     2701 tccagacatc gccatgatag ggatgggtga acggcggaat gccggtgcca tgaatggcaa
     2761 aactggaaaa cgcgcccagc ggtctgtagt cgccggcgtc ggtgagcgcg tcgatgcgca
     2821 ccagggtgat cagcggattc actgcgcgca gggccgccgc agcatcttca cttttgtcgg
     2881 tgacattctc gttgcgcacg taagcgccca tcgcgcggtt tttggtggca ccgtagattg
     2941 ccgtgctgcc aatggcgatc ttcgccggac gccggctttc ataggcagcc atcaccgcat
     3001 cggccatctg cgtcgcaaga aaatcaaaca gcgccggatc aaagcccggc ttgttggaac
     3061 caaacgtgtt gtagaaatca ctgtccatga actggccggg gccgctgtga gtgtgggtca
     3121 cgtgcagcga caggttgtgc atggccacgt cagtgcgggc ggcgatcagg tccgccacct
     3181 tgtgatgcag caccagtgaa ctggcgggca ggtccgcctg caataccaca atgggctcgc
     3241 cctgcttggg cttcagatag aaggcgcggg tcttaaggcg gttgcggaaa ccatccgcct
     3301 cccgcgccca ggcggagtaa cccattttgg gaatgccaac gggcggcgtg atgtcgcgct
     3361 cggccacgcc cgccagcagg gtctcgccgg gaatcaccgg aagcggtgcc gtgcgctgga
     3421 tgcggatttc ggtaacggga aacatgacga agggccagac ggtgaagatg ccgcccccca
     3481 atgccagcag gaccagcaac aacaggccgt agcccagtct tttcagaagt gtgcgcaaag
     3541 ggaatccgcc gcgatgccgg ttattatttt gaacgatcgt accaaatcaa gcaaaggctg
     3601 ccaacccgac gaaaaccgcg gagggtcagc gcaccacatc cgccacgacc ggggccggag
     3661 ctgctccgtg ctggaacggc ggccggcctg actgcaggta ccaacccagc acgagtgcaa
     3721 cgccaaccac tgccaccaac cgccagattg gggtgtgact ggaagtctgg acacgcgatg
     3781 tcggccggat ggatgccttg ccgctgcgca tgggcaccga ctggcgattg gcgcgccgca
     3841 cgggcgcatg gccgatatgg cggccagcgg cgggggaatg cagactcatg ttctgcgtcc
     3901 gttcactcag gcccgacagc ggcaccggcg caatccggca gttcacgccc agctgctgca
     3961 gcaggttctt caactgccac gcggtctgat ggtccaccag ctcggtgagc aggcctccct
     4021 gactggccag caactgttcc acctgggcgg gcgtgatgcc gaaccggttg ccaacggcgg
     4081 cacgcacccg ctcggggtga tagccctcgg ccacaccgtt cacgatgatt ttgaacttgg
     4141 gtgaagatga catgtgattc ccccgaaccc tcgaatgccc cccggcaggg ttcacgtacg
     4201 ctgtgccgaa actgcgtcca atcatgacgg acctgcagca aacggggtag tgaatgcgca
     4261 gcatcataaa gtcaaaaaag tacggaaaat gtgcagcagg tcgaatattc aaaagatgac
     4321 caggagcggt gcctgaaagc agacgcggct aaaaaagatt caagccggaa tcccggcgga
     4381 taaccgaaat ccagacggca gtaccagggg aaaccttttg atacacaccc tggtttgtga
     4441 ctgatcagcc agaccactac cattccaata cttgtccaaa ttggccacgg aatcggcagt
     4501 gcatgcgtct ccgcggccgg ccgctcatac gaaggtgtct tccgccatgc tgaaaaaagc
     4561 gttctacagc cgcccggaac tggaaaattt cctgcacgcc aatgaaacgg aagggccgca
     4621 actgccccag ggcaatctgc gcatgatgga ccgggtggtg tcgatctcgg agaccgaggg
     4681 ccgctaccag cgcggcgccc tggtggccga atacgacctg gacccgcagc aatggttttt
     4741 cagctgtcat tttcccggcg atcccgtgat gccagggtgc ctggtgctgg atggactgtg
     4801 gcagtgccta ggcttctacc tggcgtggcg tggctatcac ggcaaagggc gggcgatcgg
     4861 cgtggacacg gtgcggtttt tcaacgagat ccggccccag tccggcagcg tgcaatatcg
     4921 cctgcatgtg cgccgcatcc tgacacggga tttcaccctg ggtgtggcgg acggcgaagt
     4981 ctgggtgggg
//
LOCUS       DASUEG010001024         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1517922, whole genome shotgun sequence.
ACCESSION   DASUEG010001024 DASUEG010000000
VERSION     DASUEG010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517922"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VFX11_19230"
     CDS             complement(<1..1493)
                     /locus_tag="VFX11_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940815.1"
                     /translation="MFCSGWLRRRGHAGLALWSICISLMLSPGPVVAVSLVPQTCDLP
                     AYGNWNNGMRPDGYGVDLQLEWVKQGHRISPWLPLPLPHQTLQVSRYESALREYRARR
                     LPITFTGTQWEYLLSEQPYLRIKDDTNPNVLGHNGLNERRLSPFGSEKYWYEVGKSWT
                     DREMFRQLANIYPDPPQVVLLSNNEHPLLGWRELERDRRFVSRYGSKLTDEQKRELAA
                     SLWEKLYQALHQGMRDGLAATPWKDRVRLVGFLANGSMNMGRDSGWMFERLHTRDRID
                     PYSRFWDGSSYDIYYNSEETLLRSAPYRAMNSVFMVEENRRTKRDYYAELSIWDGHTY
                     DRQDWRRHQENKQPGSSLSAFVALGTLGMWLLKPEVVREFRFYDESYHNNREYTQGIM
                     ARIDAVSSDPVLKSFWCNGELVKNVRVVHPYQHSVPAKYQAESRWYFLDVEPASASPA
                     RANPDRYAAAIALRKGRVPDRSWLVYGYSYGAVSGGTAARLMLPDFG"
     gene            complement(1498..2274)
                     /locus_tag="VFX11_19235"
     CDS             complement(1498..2274)
                     /locus_tag="VFX11_19235"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX4940816.1"
                     /translation="MERKIDYIDAMPVGNTQARIRTGQPERSTEIQYPIRYSRTRIHQ
                     PSAALLERNRILHEGSTGDVVYAYKMLRTQVLKKLQDNGWNSVGLIGARAQQGATLTA
                     INLATAIAMDPRHTALLVDLNLRNPAVHRYFGYEPAQGVEDVLLAGASVESALFNPGV
                     EGLLVLPARAPCLNSSELLSGPEMHRLIVDLKQRYASRIIVFDLPPVLEAEDSISFAE
                     YYDAGLLVIADGVTSRADVERTANLVGRKPMLGSVFNNAF"
     gene            complement(2285..>2833)
                     /locus_tag="VFX11_19240"
     CDS             complement(2285..>2833)
                     /locus_tag="VFX11_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX4940817.1"
                     /translation="SKSRLQRKLEAHEAALQNAPRVEKEYRDLLRDLDNTSSKYQEVK
                     AKQMEAQMARTLETERKAERFTLIEPPLVPQRPVSPNRPLIFGFGIALALGVAAVLVW
                     LLGRVDGTVRSAQDVLKIIGVEPLVTIPHIVTLAETSTRKRKRVLASVAGFGVVLVAM
                     VVTHFAVAPLDVLWYVTLRKLG"
BASE COUNT          604 a          873 c          752 g          604 t
ORIGIN      
        1 attccaaaat ccggcagcat cagccgggct gcggttcccc cactgacagc gccgtagctg
       61 tagccataca ccagccacga ccggtcaggc acccggccct tgcgcaaggc aatggcggca
      121 gcatagcgat ctgggttggc acgggctgga cttgccgatg cgggctcgac atcaagaaag
      181 taccagcgcg actccgcctg gtattttgct ggaaccgaat gctgatacgg atgcaccacg
      241 cggacattct taaccagttc accgttgcac cagaatgatt tcagtaccgg gtccgacgac
      301 accgcgtcaa tgcgagccat gattccctgc gtgtattcgc ggttgttgtg gtagctctcg
      361 tcgtagaaac ggaattcgcg gaccacttcc ggtttgagca gccacatgcc gagcgtgccc
      421 agcgccacga aggcactcag acttgatccc ggttgtttat tctcctgatg ccggcgccag
      481 tcctggcggt cataagtatg cccatcccaa atggaaagtt ccgcatagta gtcgcgcttt
      541 gtcctgcggt tttcctcaac catgaacacc gaattcatcg ctcggtacgg cgccgaccgc
      601 agcaacgttt cctcgctgtt gtagtaaatg tcataactcg aaccatccca gaaccgggaa
      661 tagggatcga tgcgatcacg ggtgtgcaga cgctcgaaca tccagccgct gtcgcggccc
      721 atgttcatgc tgccgttggc cagaaacccc accagtcgta cccggtcttt ccacggtgtt
      781 gccgccagac cgtcccgcat tccttgatgc agtgcctggt acagcttctc ccacaaggac
      841 gcggccagtt cgcgcttctg ttcatccgtc agtttgctgc catagcggct gacgaaacgg
      901 cgatcgcgtt cgagttcgcg ccagcccaat agcggatgct cgttgttgga aagcagaacg
      961 acctgtggcg ggtcgggata aatatttgcc aactgccgga acatctcgcg atcggtccag
     1021 cttttgccaa cctcatacca gtatttttca ctgccaaacg gtgacaggcg ccgttcgttg
     1081 aggccgttgt ggccaagcac attgggattg gtatcatcct tgatacggag atagggctgt
     1141 tcgctgagca ggtattccca ctgggtgccg gtaaacgtga taggcaaacg gcgggcgcgg
     1201 tattcacgca aggcactttc ataccgggag acctgcagcg tctggtgtgg cagcggcagg
     1261 ggcagccagg gtgaaatccg gtgcccctgc tttacccact ccaactgcaa atccacaccg
     1321 taaccatcag gcctcatgcc gttgttccag ttgccatagg ccggcaagtc gcaggtctgc
     1381 ggcaccagac tgaccgccac tacaggcccc ggactcagca tcaggctgat gcagatactc
     1441 cacagagcca gaccagcatg gccacgccga cgcagccaac cggaacagaa catccagcta
     1501 gaatgcattg ttgaacaccg atccaagcat cggtttgcga ccaaccagat ttgcagtccg
     1561 ttcgacgtca gccctggagg tgacgccatc ggcgatgaca agcaacccag catcatagta
     1621 ttccgcaaag gaaatgctgt cttccgcctc cagtactggc ggcagatcaa acacgatgat
     1681 tctggatgca tagcgctgtt tgagatccac tatcagccgg tgcatctccg gaccactaag
     1741 caactcggat gaattcaggc acggcgctcg tgcaggcaat acgagcaatc cttcgacacc
     1801 gggattgaac agcgcgcttt ccacactggc gcctgccagc aggacgtctt cgacaccctg
     1861 tgccggctca tagccgaaat agcgatggac agcaggattt ctgagattca ggtccaccag
     1921 caacgcggta tggcgcgggt ccatggcgat ggccgtagca agattgattg ccgtcaacgt
     1981 ggcaccctgt tgcgcccgcg caccgatcaa ccctacgctg ttccagccat tgtcctgcaa
     2041 cttcttcagc acctgggtgc gcaacatctt gtatgcatac accacgtctc cagtgctacc
     2101 ttcgtgcaga atgcgattgc gctcaagcag ggctgcagac ggttgatgga tccgggtgcg
     2161 cgaataccgg atcggatact ggatttccgt actgcgctcc ggctggcccg tacgtatcct
     2221 ggcttgagtg ttgccaaccg gcatcgcgtc aatgtaatca attttccgtt ccacctgcac
     2281 tctcctaacc caacttgcgt agtgtcacgt accacagcac atccaacggt gcgacagcaa
     2341 aatgtgtcac caccattgca accagaacca caccaaatcc agccaccgag gccagcaccc
     2401 gttttctttt cctggtgctg gtctctgcca gcgtcacgat gtggggaatg gtcaccagtg
     2461 gctcgacccc aatgattttc agcacgtcct gagctgatcg aacagtgcca tccaccctgc
     2521 ccaacagcca caccagaacc gccgcgacgc ccagcgccag cgctattcca aaaccgaaaa
     2581 tgagtggccg gttcgggctc accggacgtt gtggcaccaa gggcggttca atcagcgtga
     2641 aacgctcagc tttacgctcc gtttccagtg tgcgcgccat ctgggcttcc atctgcttgg
     2701 ctttgacttc ctgatacttg ctggacgtgt tgtcgagatc gcgcaacaga tcgcggtatt
     2761 ccttttcaac cctgggcgcg ttctggagtg cggcttcatg agcttccaat ttgcgctgca
     2821 agcgtgattt gct
//
LOCUS       DASUEG010001025         8616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1318913, whole genome shotgun sequence.
ACCESSION   DASUEG010001025 DASUEG010000000
VERSION     DASUEG010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318913"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFX11_19245"
     CDS             <1..963
                     /locus_tag="VFX11_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4940818.1"
                     /translation="TVRDPNNEKSVAPLKKIAAAAPQGSLKLFKADLLDPTAFDAPMQ
                     GCEVVIHTASPFVVRGFKDAREALIRPAVEGTRNVLEAVNRTQSVKRVVLTSSVAAVF
                     GDNADLKKNPRGMFNEDDWNFSSSETHQPYSYSKFLAEKEAWKIHDAQQRWQLVTINP
                     SMVGGPALTKSSASTSIDTLRGLGNGRLWPGVPFMRLGWVDVRDVAQAHVEAAFRPDA
                     EGRHILSNGEPSMLEIANILRRHFGSAYRFPVFELPKFVVKWFGRFMDSSATPKFIEQ
                     NAGHDLKFDNTRSRQRLGIQYRPLEQTFVDHFQQLLDDGLIRKK"
     gene            1127..1894
                     /locus_tag="VFX11_19250"
     CDS             1127..1894
                     /locus_tag="VFX11_19250"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX4940819.1"
                     /translation="MTSLGEAQLPLLFKEGDYSIFLFVVSKRKVESLLRGTGLRPAIT
                     FGNRTFAALVLGHYSITSYKPFRFASLAISAERQFGFRPTSPWKEIFSRADQRHTGFY
                     FLNSLLDNQDMVTMGKEIWGFPHSFGDIQLAIENSRIRSHVECAHSRKTLFGLDSLGL
                     RLWRMSALEFTMFSVRGRQILRTLFNTRCTLDMSLPLQFRLRLGDSPHPIVQNLRLLG
                     LDNRRPLLCFSTHQFQGRIHEGSVVENLDDAPRSSSV"
     gene            complement(1901..4999)
                     /locus_tag="VFX11_19255"
     CDS             complement(1901..4999)
                     /locus_tag="VFX11_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4940820.1"
                     /translation="MKFTDIFIRRPVLAVVINLIIVIAGLQAIGSLNVRQFPRSDNAA
                     VTITTAYIGADAELVRGFITTPLERAIAAADGIDYVESQSSLGVSTITARLQLNYDPV
                     KALAEISSKVDQVRGDLPPESEVPVIDVVSADSEFASGYLSFTSKIMEQNQITDYLIR
                     VVQPRLAALSGVQRAEILGARTFAMRIWLHPEKMAALNVTPAQVRQALASNNVLSAVG
                     QTKGSLVQVNLTTDTNLQSVRDFQRLVVREQGGAIIRLEDIADVVLGAEDYTSDVRYS
                     GETAVFIGLWALPNANSIDVLKRVRAEMELIKRSLPGGLEGMVSYDATDYINSAIKEV
                     VKTLSETLLIVILVIFLFLGSMRSALIPVIAIPLSLIGAIFLIQLFGFTVNLLTLLAI
                     VLSVGLVVDDAIVVVENVERHLSEGQSPMQAAILGARELVSPIISMTITLAAVYLPIA
                     LQGGLTGSLFREFALTLAGAVVISGVVAVTLSPMMSSQLLKPGMDKQGLAGVVHNLFE
                     RLKAVYRRSLVATMDNRKPIYAVWIVFSVLTVPMFMFSAKELAPMEDQGIIFGIIDAP
                     ANATLEQTTHFTAQASRAFLSLPETDFTFQFTNPSGGFCAMAALPWDQRERTVFDMMP
                     ELTEKLGVIPGIQTFPVAPPALPGGGGFPVEFVIASTAPATQMVDFAQQLQQKAASSG
                     MFAFPPMIDVKYDQPQATLHIDREKVADLGLTMAQVSQDLGAMLGGSYVNRFDLDGRS
                     YKVIPQIQRSQRMTPEQLQDIHITGPDGELVPLSTIATISTKTVPRTLNRMQQMNSVK
                     ISGVAIRPLDDALRYLEDEAMKILPKGYTIDYTGESRQLRVEGNKFLPAFSMALALIF
                     LVLAAQFNSFRDPFVILAGSVPLALFGALIFTFLKMPAPDMPFWTNGWTTTLNIYSQV
                     GLVTLVGLVSKNGILIVEFANQLQKQGMAQKEAVIEAAVTRLRPIMMTTVATIAGHFP
                     LVLVTGAGAAARNSIGLVLVGGMAVGTMFTLFVVPAIYLLISRKRKARVVEEESMQAQ
                     TA"
     gene            complement(5013..6137)
                     /locus_tag="VFX11_19260"
     CDS             complement(5013..6137)
                     /locus_tag="VFX11_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4940821.1"
                     /translation="MKKHTLYTLLSIPAAIGLLAGIKFMQFDSMMKAGENYQPPALLV
                     TYASVQELQWESLMHAVGTVEAEDGIVVQAELPGKITKLAFTPGATVKEGDLLVQQDI
                     SIEQTSLRSAEAAAELARSNLQRAQRMIKQNGVSASDFDTSAAQAKQSEAQVENVKAQ
                     MAKKSIRAPFAGRLGVRQVSIGQDLRDGDPIVTLQKLDPIMVNFSLPQRDLARLQTGL
                     TVRVYSADFPDLQLQGTITAINPSVEKATRNVKVQAQLRNPGERLLPGMFVEVDVVLP
                     DPQKVLAIPATAILYAPYGDSVFVIEDRPEDQGGGKIVRQQIVKTGISRGDFIAITQG
                     LQPNATVVSTGTFKLQNGQVVTPDNTHSPEFSLTPKPDNS"
     gene            complement(6342..7307)
                     /locus_tag="VFX11_19265"
     CDS             complement(6342..7307)
                     /locus_tag="VFX11_19265"
                     /inference="COORDINATES: protein motif:HMM:NF024107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4940822.1"
                     /translation="MPRTAFRIFLLISLLFSLFAAGCRQASPPPTTPDPSALDEPGPF
                     QMAKYNTSLANELGRYPATVYYPAQTTSDGLVPDHSQGPYPAVTFSPGLASNKSFNRW
                     VGIHLATHGYVVLIFTVPNPLLLRTHQQEAGFAIGFDKLAAENANPASPLHNLVDLEH
                     RAIMGHSLGAMASLRAAAYMNVDAVVPLAPYTIETEALAAITAPTQIQAATQDCITGP
                     DKAKIDYDNLGAPIRQLVTINGGNHVSFNDANSLAETIGGPLFDCPKVIDTADHHQRL
                     SRRYFTAWLDYFLKGHTEVEEFLFGEPAQQDLLTELLTALEFMRP"
     gene            7619..>8616
                     /locus_tag="VFX11_19270"
     CDS             7619..>8616
                     /locus_tag="VFX11_19270"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEX4940823.1"
                     /translation="MHTNLSRFFLILFGVLGALVFAIGLRYGGGEHFPDRSGAPELPA
                     STLEVVANLDLPPGNIAVSASGRIFFSFHPEAKPPVNVAELVDGKPVPYPAQLPDGVQ
                     YQSVLSLRVDRQNRLWVLDNAHHGTGAPRLLAFDLATNTLVHRHDFASDIAGIGSHLN
                     DFQVRADGNMIYIAEASIFAKNPALVIYDVEKQTARRVLEGHDSVVADYFTPVVQGRR
                     MLMLGIFAIRPGVDSIALSRDGEWLYFAPVTDDKLWRIRRELLDDPALDTTTLAQSVQ
                     PYFPKTMSDGITTDDAGNVYLSDADNSAIVLLTPQGQLRTLLKDDTLRWPDGFSFGP"
BASE COUNT         1658 a         2520 c         2649 g         1789 t
ORIGIN      
        1 accgtgcgtg atcctaacaa cgaaaaatcc gttgcaccgc tgaagaaaat tgccgcagcc
       61 gcgccgcagg ggtcgctcaa gctgttcaag gccgacctgc tggacccgac cgcgttcgat
      121 gcgccgatgc agggctgcga agtcgtcatt cacaccgcgt cacctttcgt ggtgcgggga
      181 ttcaaggatg cgcgggaagc cctgatccgc ccggcggtgg aaggaacccg caacgtactg
      241 gaggcagtga atcgcacgca gtcggtgaaa cgggtagtgc tcaccagcag tgtggcggcg
      301 gtgtttggcg acaacgccga cctgaaaaaa aatccgcgcg gcatgttcaa tgaggatgac
      361 tggaattttt ccagcagcga aacccatcag ccgtattcct attcgaaatt cctggcggaa
      421 aaggaagcct ggaaaatcca cgacgcacag cagcgctggc agctggtgac aatcaatccc
      481 agcatggtgg gcggcccggc cctcaccaaa agcagtgcgt ccaccagcat tgataccctg
      541 cgcggcctgg gcaacggccg gctgtggccc ggcgtgccgt tcatgcggct gggctgggtg
      601 gacgtgcgcg acgtggcgca agcccatgtg gaagcggcgt tccgcccgga cgcggaaggg
      661 cggcacatcc tgtcgaacgg cgaacccagc atgctggaga tcgccaatat cctgcgccgg
      721 catttcggca gcgcctaccg gttcccggta tttgaattgc ccaagttcgt ggtgaagtgg
      781 tttggccgct tcatggattc cagtgccacg ccgaaattca tagagcagaa cgccggccac
      841 gacctgaaat tcgacaacac ccgcagtcgt cagcgtttgg gtattcagta ccggccgctg
      901 gagcagacct tcgtggatca cttccagcaa ttactggacg acgggctgat ccgcaaaaaa
      961 tagggtggcc gtgatggtgg acgtctgccc ggtcacattt acggaggaac ggcgttactg
     1021 cacggtcgtg ctgtgttagg ctggccatct ttgacatgac tgccaaattt atcagcccag
     1081 cgccatgcag attgatccgt tttttcaggt tccccagcac gcggtaatga catcgctcgg
     1141 cgaggcccag ttaccgctgc tgttcaagga aggggactac agcatttttt tgtttgtggt
     1201 cagcaagcgc aaggtggaat ccctgttgcg gggaaccggc ctgcgtcctg ccatcacgtt
     1261 tggcaaccgc acgttcgcgg ccctggtgct ggggcattac tcgatcacca gctacaagcc
     1321 gttccggttt gcctcgctgg ccatttcggc ggagcgccaa ttcggtttcc ggcccacctc
     1381 gccctggaag gaaatctttt cccgcgcgga tcaacgccac acggggttct attttctcaa
     1441 cagcctgctg gacaaccagg atatggtgac gatgggcaag gagatctggg gctttccgca
     1501 cagcttcggc gacattcagc tggccatcga gaattcccgc atccgcagcc acgtggaatg
     1561 tgcgcattca cgcaaaaccc tgtttggtct tgacagcctc ggcctgcggc tctggcgcat
     1621 gtcggcgctg gaattcacca tgttttcggt gcgtggcagg caaatcctgc gcacgctgtt
     1681 caatacccgc tgcactctgg acatgtcgct gccgctgcaa tttcgcctgc gcctgggtga
     1741 cagcccacat ccgatagtgc agaacctgcg cctgctgggg ctcgacaacc ggcggccgtt
     1801 gctgtgcttc agcacccatc aatttcaggg ccgcattcac gaaggcagtg tggtggagaa
     1861 tctggacgac gcgccgcgtt cgtccagtgt gtagtcgggc tcaggctgtc tgcgcctgca
     1921 tggattcctc ttccaccacc cgagccttcc tcttcctgct gatcagcaaa taaatcgccg
     1981 gtaccacaaa cagcgtgaac atcgtgccca ccgccatgcc gcccaccagc accaggccga
     2041 tggagttgcg cgcggccgca ccggcaccgg tcaccagcac cagcgggaaa tgcccggcaa
     2101 tggtggcaac cgttgtcatc atgatgggcc gcagtcgtgt caccgccgct tcgatcaccg
     2161 cttctttctg cgccatgccc tgcttctgca gctggttggc gaattccacg atcagaatgc
     2221 cgttcttcga taccagccct accagcgtta ccagtcccac ctgcgaatag atgttcagcg
     2281 tggtggtcca gccgtttgtc cagaatggca tgtccggtgc gggcattttc agaaaggtga
     2341 aaatcagcgc gccgaacagc gccaggggta ctgatcctgc cagaatcaca aacggatcgc
     2401 ggaagctgtt gaactgcgct gccagcacca gaaaaatcag ggccagcgcc atgctgaacg
     2461 cgggcaggaa tttgttgcct tccacccgca gctgacgcga ctcgccggtg taatcgatgg
     2521 tgtaaccctt gggcaggatc ttcatcgctt cgtcttccag gtaacgcagg gcatcgtcca
     2581 gcggacgaat ggctacgccg ctgatcttta ccgagttcat ctgctgcatg cggttgagcg
     2641 tgcgtggcac ggtcttggta ctgatggtgg cgatggtgct gaggggaacc agttcgccat
     2701 caggtccggt gatgtgaata tcctgcaact gttccggtgt catgcgctgg ctgcgctgga
     2761 tttgcggaat caccttgtag ctgcggccat ccagatcgaa gcggttgacg taactgccgc
     2821 ccagcatggc acccagatcc tggctcacct gtgccatggt caggcccagg tccgccactt
     2881 tctcgcgatc gatgtgcagg gtggcttgcg gctggtcata tttcacgtcg atcatcggcg
     2941 gaaacgcgaa catgccgctg ctcgctgcct tctgctgcaa ctgctgcgcg aaatccacca
     3001 tctgggttgc gggtgcggtg gaggcaatca cgaattccac gggaaaaccg ccgccgcccg
     3061 gcagcgcggg tggtgccacc ggaaacgtct gaatgccggg aattacgccc agcttttccg
     3121 tcagttccgg catcatgtca aagaccgtgc gctcgcgctg gtcccacggc agggcagcca
     3181 ttgcacagaa gccgccgctg ggattggtga actggaaggt gaagtccgtt tccggcaggc
     3241 tgaggaacgc gcgtgaagcc tgagccgtga aatgggtggt ctgttccagt gtggcattgg
     3301 caggcgcatc aatgatgccg aagatgatgc cctggtcttc cattggtgcc agttccttcg
     3361 ccgaaaacat gaacatgggc acggtgagaa cgctgaacac gatccacacg gcatagatag
     3421 gtttgcggtt gtccatggtg gccaccaggc tgcggcggta gacggctttc aaccgttcaa
     3481 acaggttgtg caccacgcct gccaggccct gcttgtccat gccgggtttg agcagctgcg
     3541 acgacatcat gggcgacagc gtaacagcca ctacaccgga aatgaccact gcacccgcca
     3601 gcgtcagcgc aaattcgcgg aacagcgagc cggtgagtcc gccctgcaat gcgatgggca
     3661 gatacacggc cgccagtgtg atcgtcatgg aaatgatcgg gctcaccagt tcgcgcgcgc
     3721 ccaggatggc cgcctgcatg ggtgactggc cttcgctcag atgccgttcc acgttttcca
     3781 ccaccacgat ggcatcgtcc accaccagcc ccaccgacag cacgatggcc agcagcgtca
     3841 gcaggtttac cgtgaagccg aacaactgga tcaggaaaat ggcgccgatc agcgacagcg
     3901 ggattgcgat caccggaatc agtgccgacc gcatcgatcc cagaaacagg aagatcacca
     3961 ggatcacgat cagcagcgtt tcgctcaggg ttttcaccac ttccttgatg gcgctgttga
     4021 tgtaatcggt ggcgtcgtag gacaccatgc cttccagtcc gcccggcaga ctgcgcttga
     4081 tgagctccat ctctgcgcgc acccgcttga gcacgtcgat ggaattggcg ttgggcagcg
     4141 cccagaggcc gatgaatacc gctgtttcgc cggagtagcg cacgtcgctg gtgtaatcct
     4201 cggcgcccag caccacgtcg gcgatgtcct ccaggcggat gatggcgccg ccctgttcac
     4261 gcaccaccag gcgctggaaa tcccgcaccg actgcaggtt ggtgtcagtg gtcaggttca
     4321 cctgcaccag cgagcccttg gtctggccca ctgcgctcag cacgttgttg ctggcgagcg
     4381 cctgacgcac ctgcgccggc gtcacgttca gcgccgccat tttttccgga tgcagccaga
     4441 tacgcatggc aaaggtgcgc gcgccgagaa tctcggcacg ctgcacgccg gagagcgctg
     4501 ccagtcgcgg ttgcaccacg cggatcaggt aatcggtgat ctggttctgc tccatgatct
     4561 tcgacgtgaa gctcaggtag cccgacgcga attcactgtc ggcggatacc acgtcgatca
     4621 ctggcacttc cgattccggc ggcaggtcgc cgcgcacctg gtctaccttg gaactgattt
     4681 ccgccagcgc tttcaccgga tcgtaattca gttgcaggcg cgcggtgatg gtggagacgc
     4741 ccagcgaact ttgcgattcc acgtagtcga tgccatcggc ggcggcgatg gcgcgttcca
     4801 gcggcgtggt gatgaaaccg cgcaccagtt cggcatcggc accgatgtag gcggtggtga
     4861 tggtgacggc ggcgttgtcg ctgcggggaa actggcgcac gttgagtgag ccgatagcct
     4921 gcaggccggc aatcacaatg atcaggttga tcaccaccgc cagcaccggc cggcggatga
     4981 aaatgtcggt gaatttcatg gtggcggccc tgtcaggaat tatcgggttt cggtgtcagg
     5041 ctgaattcgg gagagtgcgt gttgtcgggt gtcacgacct gtccgttctg cagtttgaag
     5101 gtgccggtgg agaccacggt ggcattgggc tgcaggccct gggtaatggc aatgaaatct
     5161 ccgcgggaaa tgccggtttt cacgatctgc tggcgcacga tcttgccgcc gccctggtct
     5221 tccgggcggt cttcaatcac gaacactgaa tccccgtagg gcgcatacag aatggccgtg
     5281 gcggggatgg ccagcacctt ttgcggatcg ggcagcacca cgtccacttc gacgaacatg
     5341 ccgggcagca ggcgctcgcc cggattgcgc agctgggcct gcaccttcac gttgcgggtc
     5401 gccttttcca cgctggggtt gatggcagta atggtgcctt gcagctgcag gtccggaaaa
     5461 tcggcggaat acacccgcac ggtgaggccg gtttgcagcc gtgccagatc ccgctgcggc
     5521 agggaaaaat tcaccatgat cggatcgagc ttctgcaggg tgacaatggg atcaccgtca
     5581 cgcagatcct ggccgatgct cacctggcgc acgcccagtc gccctgcaaa tggcgcgcgt
     5641 atggatttct tcgccatctg cgccttgacg ttttccactt gagcttcact ctgcttcgcc
     5701 tgcgccgccg atgtatcgaa gtccgatgcc gacacgccgt tctgcttgat catgcgctgg
     5761 gcccgctgca gattgcttct ggccagctcg gccgctgcct cggcagaacg cagcgatgtc
     5821 tgctcgatgg aaatatcctg ctgcaccagc aggtcgcctt ccttcacggt ggcaccgggc
     5881 gtgaaggcca gcttcgtgat cttgcccggc agttctgcct gcaccacgat accgtcttcg
     5941 gcctccaccg tgcccactgc gtgcatcagc gattcccact gcagttcctg cacgctggca
     6001 taggtgacca gcagcgcggg tggctggtag ttttctccgg ctttcatcat ggaatcgaac
     6061 tgcatgaact tgatgccggc cagcaggccg atggcagcag gaatactcag cagcgtgtag
     6121 agggtgtgct ttttcatgca gactccagag tcgaaaaaaa atcagcccgc gcgcgcaccg
     6181 ggcgacgaac ggaccgacat attagcctga ttttctggag gatggcgatg gtgatggatg
     6241 ccggatactg cggaaaaatt cacgtacaat gagaagggat tgcgtcgata ttcgccctgc
     6301 cgatgacaat aacggccacc tgcagggcgc aatggcgatg atcagggccg catgaattcc
     6361 agtgctgtca gcagctctgt cagcagatcc tgttgggccg gctcgccgaa cagaaattct
     6421 tccacttccg tgtgtccttt cagaaaataa tcgagccacg cggtgaaata gcggcgcgac
     6481 aggcgctggt gatggtcagc ggtgtcgatt accttcgggc agtcgaacag cgggccgccg
     6541 atggtttccg ccagactgtt ggcgtcgttg aagctgacat ggttgccgcc gttgatagtg
     6601 accagctgcc tgatgggtgc acccagattg tcataatcga tttttgcctt gtcggggccg
     6661 gtgatgcagt cctgggtggc ggcctggatc tgggtgggtg cggtgatggc ggcgagcgct
     6721 tcggtttcaa tggtataggg cgccagcgga accacggcat caacattcat gtacgcggcc
     6781 gcccgcaggc ttgccatggc gccgagggaa tggcccatga tcgcacggtg ttccagatcc
     6841 accagattgt gcagcgggct ggccggattt gcattttccg ccgccagttt gtcgaagcca
     6901 atggcgaaac cggcttcctg ctggtgagtg cgcagcagca gtggattggg cacggtgaaa
     6961 atcagcacga cataaccgtg ggtggccaga tgaatgccga cccagcggtt aaaactttta
     7021 ttggatgcca gccctggcga gaaggtgaca gcggggtagg ggccctggct gtgatccggc
     7081 accagtccgt cgctggtggt ttgtgccggg tagtacacgg tggcggggta gcggcccagt
     7141 tcattcgcca gtgacgtgtt gtattttgcc atctggaacg ggccgggttc atccagtgcg
     7201 ctggggtccg gcgtggtggg tggtggtgag gcctgccggc aaccggcggc aaacagggaa
     7261 aacagtagtg atatcagtag gaagatgcgg aaggcagtgc gcggcatagg gatgaccttt
     7321 tttgttgtag gttcatgggt ggggtcggtg gacggggaca acaggtccga ctgtaacaat
     7381 cgtggtgata cggcttcatc gtccaaaagc attagcccgc ttgtcagcag gggctggctt
     7441 gcagcgtgga tggccggctt ttatgctgca ttgattctgg aatggtggac caggcagatt
     7501 caaagcgggg attcaccttc aaaatacgca gtggctgaga tccgtagtcc cgtgcgatcc
     7561 ggtgtaccag gcccggctat ttcaaacccg gctatttcaa aagctaacgg agcgcagcat
     7621 gcacacaaac ctttcccgtt tttttctgat tctgttcggc gtgctggggg cgctggtgtt
     7681 tgccatcggg ttgcgctacg gcggtggtga acattttccc gatcgcagcg gtgcgccgga
     7741 actgccggct tccaccctgg aagtcgtggc caatctcgac ctgccgccgg gcaatatcgc
     7801 agtatctgcc agtggccgca tttttttcag ttttcatccg gaagccaaac ctccggtgaa
     7861 tgtcgcggaa ctggtggacg gcaagccggt gccgtatcca gcgcaattgc cggatggagt
     7921 gcagtaccag tctgtattgt cgctgcgggt ggaccggcag aatcgtctgt gggtactgga
     7981 caacgcccat cacggcactg gcgcaccgcg cctgctggca ttcgatctgg ccaccaacac
     8041 gctggtgcat cgccatgatt tcgccagcga catcgccggc atcggttccc acctcaacga
     8101 tttccaggtg cgtgccgacg gcaacatgat ttacatcgcc gaggccagca tctttgcaaa
     8161 aaatcccgcg ctggtgattt acgatgtgga aaaacaaacc gcgcgccgcg tgctggaagg
     8221 ccacgattcc gtggtggcgg attatttcac tccggtggtg caggggcggc gcatgctgat
     8281 gctgggcata ttcgcgatcc gcccgggtgt ggattccatt gcgctgagcc gtgacggcga
     8341 gtggctgtac ttcgcgccgg tgaccgacga caaactgtgg cgcatccggc gtgaattact
     8401 ggacgacccg gcgctggata ccaccacgct ggcgcaatcc gtgcagccgt attttcccaa
     8461 aaccatgagc gatggcatta ccactgacga tgccggcaat gtctatctca gcgacgccga
     8521 caattccgcc atcgtgctgc tgacaccgca gggccagctg cgcacgcttt tgaaagacga
     8581 cacattacgc tggcccgatg gcttcagttt tggtcc
//
LOCUS       DASUEG010001026         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1892648, whole genome shotgun sequence.
ACCESSION   DASUEG010001026 DASUEG010000000
VERSION     DASUEG010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892648"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFX11_19275"
     CDS             complement(<1..959)
                     /locus_tag="VFX11_19275"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX4940824.1"
                     /translation="MTVIKQDDLIQSVADALQYISYYHPVDFIQAINAAYEREQNQAA
                     KDAMAQILINSRMCAEGHRPICQDTGIVTVFVKVGMDVRWDLGGMSLDDAINEGVRRA
                     YLNPDNVLRASILRDPAGKRANTKDNTPAVIHYSIVPGDKVDITVAAKGGGSENKSKM
                     VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAALLAKESLMDPVDMHEL
                     LARGPQNRIEELRIELYEKVNALGIGAQGLGGLTTVVDIKIRDYPTHAASLPVAMIPN
                     CAATRHVHFELDGSGPAHLEVPKLEDWPKITFDASKSRRVNLDT"
     gene            complement(1094..1990)
                     /locus_tag="VFX11_19280"
     CDS             complement(1094..1990)
                     /locus_tag="VFX11_19280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4940825.1"
                     /translation="MSSIEVLTLEHEGLSFSARATGSGPLVLMLHGFPDDFHSFDAQM
                     EAIAAEGYRVVAPMMRGYETSSCSPRDLYHLIHLASDVFAWMKLLGVKQCHLVGHDWG
                     ALTSYVAAALQPKKFASLTTLAIPHLRRGLQGVAGVPSQLVKSSYILLMQFQKASEAI
                     VERDDYAFIETLWRRWSPGWHYSPADIEQVKSTFRADGVTHAATQYYRCLLQPLSLST
                     QQSWRLLTSRVSVPTLAITGEQDGCMDSRLYDSLMREQDFENGLEVCKLPNAGHFLHR
                     EAPVEVNGLILDWIRQHPVSAD"
     gene            complement(1987..3240)
                     /locus_tag="VFX11_19285"
     CDS             complement(1987..3240)
                     /locus_tag="VFX11_19285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX4940826.1"
                     /translation="MPLSIARQSLSFSRFVIRRFLEDNCRQNAMVLTYTTLFAVVPIM
                     TVMFAMLSAIPSLQHVSADIQDFVFKHFIPSSGAVVQEHLQAFSHQARGLTMIGLVML
                     VVTALMLLVTIEKSFNQIWKVHHARKGVVSFLRYWAVLSLGPLLLGAGFAISSYLASL
                     KLFSTAASVVGDAVPGLQLLPFVSTTLAFMLLYVTVPNCKVPLKAGFWGGFLAALLFE
                     LAKGAFGLFVGQFSSYTLVYGAFAAFPVFLIWIYLSWVIILLGVEITRALALYRRTGL
                     ARRHPVVALLDILQLFWRKQQRGGTVTDIEAMSILGKKEVENWFQFADILQREHIIRR
                     TDDGGYVLARNLEQIRFNDFYRSLPWPLPRPVDLEHLPADDHWVQVLKPALLTVSDTM
                     DRELGMSLASVLREDDVESSGVKSV"
     gene            3362..3709
                     /gene="arsC"
                     /locus_tag="VFX11_19290"
     CDS             3362..3709
                     /gene="arsC"
                     /locus_tag="VFX11_19290"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610449.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX4940827.1"
                     /translation="MYTIYHNPRCSKSREALALLEEAGVSVTVVRYLETPLDADALRQ
                     LLSRLGLPARDLLRSKEEEFASLGLGDPALTDDDLIAALVSHPRLMERPVVVHGRRAV
                     IARPPERVKELLK"
     gene            3706..4302
                     /gene="wrbA"
                     /locus_tag="VFX11_19295"
     CDS             3706..4302
                     /gene="wrbA"
                     /locus_tag="VFX11_19295"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX4940828.1"
                     /translation="MTTPYVLVLYYSRHGATRQMARLIARGVEKEGLEARLRTVPAVS
                     TTVEATEPVIPEEGELYCSLDDLSGCAGLALGSPTRFGNMAAPLKYFLDGTSGLWLNG
                     ALIDKPAVVFTSTSSLHGGQETTLLSMMLPLMHHGMAVLGLPYSEAGLLRTTAGGTPY
                     GASHLAGADSKRALDDTESELCIALGQRIARHARRGLS"
     gene            4299..>4652
                     /locus_tag="VFX11_19300"
     CDS             4299..>4652
                     /locus_tag="VFX11_19300"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX4940829.1"
                     /translation="MTTSRASRARKLTLVLLISLLVFMAAETFLFAPPTMSLAAILFI
                     IGIKIVPPALFILPLSRGHAMSAVWLGILLMPYFCWAVLGALVPGTEGVVAAMRGALT
                     AACVVSAMLFARWQKA"
BASE COUNT          944 a         1419 c         1393 g          896 t
ORIGIN      
        1 gtatccaggt tgacgcggcg ggacttgctg gcatcaaagg tgatcttcgg ccagtcttcc
       61 agcttcggca cctccaggtg ggccgggccg gagccatcca gctcgaagtg cacgtgacgg
      121 gtggcggcac agttggggat catggcgacc ggcagcgagg cggcgtgggt cgggtagtcg
      181 cgaatcttga tgtccactac agtggtcaga ccacccaaac cttgggcgcc gatacccagc
      241 gcgtttactt tctcgtacag ctcgatacgc agttcttcga tgcggttttg cgggccacgg
      301 gccaacagct cgtgcatgtc gaccgggtcc atcagggatt ccttggccag cagtgcagcc
      361 ttttcggccg taccaccgat gccgataccc aacatgccgg gcgggcacca gccggcgccc
      421 atggtcggca cggtcttcag cacccagtca acgatggaat cggacgggtt gagcatgacc
      481 atcttggact tgttctcgga accaccaccc ttggcggcga cagtgatgtc gaccttgtcg
      541 cccggcacga tgctgtagtg gatgacggct ggcgtgttgt ccttggtgtt ggcgcgcttg
      601 ccggccggat cacgaaggat ggaggcacgc agcacgttgt ccggattcag gtaggcacga
      661 cgcacacctt cgttgatggc atcgtccagg ctcataccgc ccaggtccca acgcacatcc
      721 atgcccactt tcacaaacac ggtgacgata ccggtgtcct ggcagatcgg gcggtggcct
      781 tcggcacaca tgcgtgagtt gatcaggatc tgcgccatgg cgtccttggc ggcctggttt
      841 tgttcccgct cataggccgc attgatggcc tggatgaagt ccaccggatg gtagtaggaa
      901 atgtactgga gggcgtcggc aacgctctgg atgaggtcgt cctgcttgat gacggtcatg
      961 gggtctccgg gctgggcgcg gttagcgggc tggcatgatc aggcaaggca ttgtgtgccc
     1021 tgcccgcagg tagtttttgg cggcgccgat tatacccaag caggcggcgg atatccgccc
     1081 cgatgtgacg ctgtcagtcg gcggacacgg ggtgctgacg gatccagtcc agaatcagcc
     1141 cgttgacctc gaccggggct tcccggtgca ggaaatgacc ggcattgggc agcttgcaga
     1201 cctcaaggcc gttctcgaaa tcctgctcgc gcataaggct gtcatacagg cggctgtcca
     1261 tgcagccatc ctgctcgcct gtgatggcca gcgtgggcac gctgacgcgg ctggtcagca
     1321 ggcgccagga ctgctgggtg gacagtgaca gcggttgcaa caggcagcgg tagtactggg
     1381 tggccgcatg ggtcacacca tcagcacgga aggtggattt cacctgctcg atgtcggcgg
     1441 gcgagtaatg ccagccgggt gaccagcgcc gccagagtgt ctcgatgaag gcgtaatcgt
     1501 cacgctcgac aatggcctcg gacgccttct ggaactgcat gagcaggatg tacgacgact
     1561 tcaccaactg gctcggcacc cctgcgacac cctgcaagcc acgccgcagg tgcggaatgg
     1621 ccagagtggt caacgaagca aacttctttg gctgcaaggc cgccgccaca tagctggtca
     1681 gtgcccccca gtcatgtccg accagatggc actgcttgac gcccagaagc ttcatccagg
     1741 caaagacatc ggatgccaga tggatgaggt ggtacagatc gcgcggcgag caggatgagg
     1801 tctcgtaacc acgcatcatc ggagccacga cgcggtagcc ctcagcggca attgcctcca
     1861 tctgggcatc gaaggaatga aagtcgtccg gaaaaccatg cagcatcagg accagcgggc
     1921 cactaccggt tgcgcgtgcg gaaaaactca aaccctcgtg ctcgagggtc agcacttcaa
     1981 tgctgctcat acagacttca ctccactgct ttccacatca tcttcccgga ggacgctggc
     2041 cagagacatg cccagttcac ggtccatggt gtcgctgacc gtcagcaacg ccggcttgag
     2101 tacctgcacc cagtgatcat ctgcaggcag atgctccaga tccaccggac gcggtaatgg
     2161 ccagggcagg ctgcggtaaa aatcattaaa acgaatctgc tccaggttgc gcgccagcac
     2221 atatcccccg tcgtccgttc gcctgatgat atgttcacgc tgcaggatat cggcaaactg
     2281 gaaccagttt tccacttctt tcttcccgag aatgctcatt gcctctatat cggtcaccgt
     2341 tcctccacgc tgctgcttgc gccagaacag ctggaggata tcgagcaaag ctactacggg
     2401 atggcgacgg gccagcccgg tgcgacgata cagggcgagc gcccgcgtga tctcaacccc
     2461 cagcaaaatg atcacccacg acaggtagat ccagatcaga aagaccggga aggccgcaaa
     2521 agcaccatac accagcgtat acgacgaaaa ctggccgaca aagagcccga acgcaccttt
     2581 ggccagctca aacaggagtg ccgccaggaa tccgccccag aaacccgcct tcaacggcac
     2641 cttgcaattg ggcacggtga catacaaaag catgaaggcc agcgtggtgg acacgaaggg
     2701 cagcaactgc aagccgggca cggcatcacc caccacagat gccgccgtac tgaacagttt
     2761 gagggaagcc agataggagg aaatggcgaa gcccgcacca agcaacagag gtcccaggct
     2821 gagcactgcc cagtaccgca ggaagctcac cacgcccttg cgtgcatgat gaaccttcca
     2881 tatctggttg aacgacttct cgatggtaac cagcagcatc aacgcagtta ccaccagcat
     2941 gaccagacct atcatggtca gccctcgtgc ctggtgcgag aacgcctgca gatgctcctg
     3001 caccaccgca cccgaactgg gtatgaaatg cttgaagacg aagtcctgaa tgtcggcgct
     3061 gacatgctgc aaggaaggaa tggccgacag catcgcaaac atgacggtca tgatgggcac
     3121 caccgcgaac agggtggtat aggtcagcac catggcattc tgccgacagt tgtcttccag
     3181 gaatcgccgg atgacaaagc ggctgaatga gagggactgg cgtgcaatcg acaaaggcat
     3241 gaaagatccg tgcagtttca ggctggccaa gtattcccgt cagggtacac gctgtcgagt
     3301 atcaccgggg cggccttcat cgctacaatg accgcctttg attgctgcaa gcatagcgcc
     3361 catgtacacc atttatcaca atccccgctg ctccaagtcc cgcgaggctc tggccttgct
     3421 ggaagaggcc ggtgtttccg ttactgtcgt ccggtatctg gagacccctc tggatgctga
     3481 tgcccttcga caactgctgt ctcgactggg tctacccgcc agagacctgc tgcgcagcaa
     3541 ggaggaagaa tttgccagtc ttggccttgg cgatccggca ctgaccgatg acgatctgat
     3601 tgccgcgctg gtctcgcatc ctcgcctgat ggagcgccct gttgtcgttc atggccgccg
     3661 cgccgtgatt gcccgtcccc ccgagcgggt caaggagcta ctgaaatgac caccccctat
     3721 gttctggtgc tgtactacag ccgtcatggc gccactcgcc agatggcccg cctgattgcc
     3781 cgtggtgtcg agaaagaagg actggaggcc agactgcgca ccgtgccggc cgtgtcgaca
     3841 accgtagagg ccacggaacc ggtgattccg gaggaaggtg aactctattg ttcgctggac
     3901 gacctctccg gatgcgccgg actggcactg ggtagcccta cccgctttgg caacatggct
     3961 gcccccctga aatacttcct ggatggcacc agcggcttgt ggctgaacgg agcgttgatc
     4021 gacaagccgg ctgtggtctt tacctccacc agctccctgc atggcggaca ggaaaccacc
     4081 ctgctcagca tgatgctgcc gctgatgcat cacggcatgg cggtacttgg gctgccctat
     4141 tcggaggccg gcctgctgcg gacaacggcc ggcggcaccc cttacggggc cagccatctt
     4201 gccggcgcgg acagcaagcg tgcgctggat gataccgaga gcgagctttg catcgccctg
     4261 ggacagcgaa ttgcccgcca cgccagacga ggactgtcat gaccacttcc cgcgcctccc
     4321 gggcacgcaa actgacgctg gtgctgctga tcagcctgct ggttttcatg gcggctgaaa
     4381 ccttcctgtt cgccccgcca acgatgagcc tggctgccat cctgttcatc atcggcatca
     4441 agattgtgcc gcccgccttg ttcattctgc ccctgtcgcg gggacatgcc atgagtgctg
     4501 tatggttggg catcctgttg atgccttact tctgctgggc tgtactgggg gccttggtgc
     4561 cgggcacgga aggtgttgtc gccgccatgc gcggcgctct aactgccgcc tgcgtggtgt
     4621 cggccatgct ctttgcccgc tggcaaaagg cc
//
LOCUS       DASUEG010001027         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1495818, whole genome shotgun sequence.
ACCESSION   DASUEG010001027 DASUEG010000000
VERSION     DASUEG010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495818"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1570
                     /gene="flhF"
                     /locus_tag="VFX11_19305"
     CDS             113..1570
                     /gene="flhF"
                     /locus_tag="VFX11_19305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX4940830.1"
                     /translation="MNTQKFNAPDMRQALKMVRDSLGADAVILSNRKVAGGVEIIAAA
                     DYGTFLAEHSAREREAHHVPPFMDASAPVVSPAQGDPARLIQPDKRQDPALAEQMKLM
                     ADQQRAQSASRQFNELLRRQLQEQRAQADAEPSVEISPQAQARQAQAQAPVKAPTPVA
                     PRVQPAPGAGSSSELAAMRNELSQLRNMLNVQLANVSWGDFSYRHPLEANVFKRLTKL
                     GLSPALCRKLTRNLAPEMDTKSAWQHALKELGEDLPLVTEDILAHGGIFAFVGPAGVG
                     KTTTIAKLATRYVLEHGADDIALVTTDSYRIAGHEQLSTLGRILKIPVRVVTEQNSLE
                     QILRGLARKKLVLIDTAGLSHKDQVHREQMKMLEAMGANIQRWLVLSSTCQRRILDKA
                     IDDYKHLNLEGCVFTKLDESASLGEALSVAIEAQLPIAYITDGQNIPDDIARPQPSRL
                     INHAIALAKENKADDSLVADLYSDALTAQSDVAYA"
     gene            1604..2425
                     /locus_tag="VFX11_19310"
     CDS             1604..2425
                     /locus_tag="VFX11_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX4940831.1"
                     /translation="MSTHPVQVIAVTGGKGGVGKSNVTINLGMCLAEMGRRVVLLDAD
                     LGLANLDILLGITARENLSHVLRGECSLRDILVDGPGGMKIVPASSGVAELVNLGTRE
                     HAGLIHAFSELSNDVDVLLIDTAAGISDMVMSFVTAAQESLFVVTNEPTSITDAYAMI
                     KLLNQQHGMHRFNILANMVRSQQEGRELYAKLSRVTDRFLDVALQFIGCIPFDEYVRK
                     AVMRQKPVVEAYPRSKAALAFRTLANKVDSWPLPTTPRGHLEFFVDRLVQGAAVR"
     gene            2437..>2775
                     /locus_tag="VFX11_19315"
     CDS             2437..>2775
                     /locus_tag="VFX11_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4940832.1"
                     /translation="MLGVNRYAMYSDESAPPLAVMVEQYTPLVKRIAHHLLARLPSSV
                     AVEDLYQAGMIGLLDAIKRFDSTKGASFETYAGIRIRGAMVDEVRKGEWAPRSVHRNA
                     RRIQEAMRAVE"
BASE COUNT          595 a          815 c          829 g          536 t
ORIGIN      
        1 tgcggttcag tgtccagggc ctgcatgtgt tgtcgtacaa cgaagtgcct gacaacaagc
       61 agatcaccat caccgcgtcg gtcggtcaat gatttcagaa aggtaaagca tcatgaatac
      121 acaaaaattc aacgcaccgg atatgcgtca ggccctgaaa atggtgcgcg actcgctggg
      181 tgccgatgcc gtcatcctgt ccaaccgcaa agtggctggt ggcgtggaaa tcattgctgc
      241 cgccgactac ggcacttttc tggcggaaca ctctgcccgt gagcgcgagg cccatcatgt
      301 ccccccgttc atggacgcca gcgcgccggt cgtttcgccg gcccagggtg atcctgcccg
      361 cctgatccag ccggacaaac gtcaggatcc ggcactggcc gagcagatga aactgatggc
      421 ggaccagcag cgcgcgcaaa gtgcaagccg ccagttcaat gaactgctgc ggcgtcagct
      481 gcaggaacag cgggcacagg ccgatgccga acccagcgtg gaaatcagcc cgcaggcgca
      541 ggcccggcag gcacaagccc aggcgcctgt caaagcgccc acgcccgtag caccccgcgt
      601 gcagccggct cccggcgccg gcagcagcag cgagctggca gccatgcgca acgaactcag
      661 tcagttgcgt aatatgctca atgtacagct ggccaacgtg tcctggggcg atttcagcta
      721 tcgccatccg ctggaggcca acgtcttcaa gcgtctgacc aagttgggat tgtcaccggc
      781 gctgtgccgc aaactgaccc gcaatctggc gccggaaatg gacaccaaat ctgcctggca
      841 acacgcattg aaggagctgg gggaggatct gccactggtg accgaagaca ttctcgctca
      901 tggcggtatt ttcgctttcg tcggccctgc cggggtgggc aagaccacca ccatcgccaa
      961 gctggccacc cgctatgtac tggaacacgg tgccgatgac atcgctctgg tgaccaccga
     1021 cagctaccgc attgccggcc atgagcagct gagcaccctc ggtcgcatcc tgaagattcc
     1081 ggtgcgggtg gtgactgaac agaattccct ggagcagata ctgcgtggcc tggcgcgcaa
     1141 gaagctggtg ttgatcgaca ccgctggcct cagccacaag gatcaggtgc accgtgagca
     1201 gatgaagatg ctggaagcca tgggtgccaa catccagcgc tggctggtgc tgtcctccac
     1261 ctgtcagcgc cggattcttg acaaggcaat cgacgattac aagcacttga atctggaagg
     1321 atgtgtgttc acaaaactgg acgaatccgc ctctttgggc gaggctttaa gtgtagcaat
     1381 cgaagcgcag ttacccattg cctacattac tgacggccag aacatcccgg acgacattgc
     1441 ccgcccacag ccatcccgcc tgatcaatca cgccatagcc ctggcgaagg agaacaaggc
     1501 cgatgacagt ctggtggcgg atctctacag cgacgccctg acggcgcaat ccgatgtggc
     1561 ctacgcgtaa cgacgctaag aaaaggtgtt tgtgaattca tttatgagta ctcatccagt
     1621 acaggtcatt gcggtgacgg gcggcaaagg cggcgttggc aagagtaacg tcaccatcaa
     1681 tctgggcatg tgcctggcgg aaatgggccg gcgcgtcgtg ctgctggacg ctgacctggg
     1741 gctggccaat ctcgatatcc tgctggggat cacggcccgg gaaaacctgt cccatgtact
     1801 gcggggcgaa tgctcgctac gcgatatcct ggtggatggt cccggcggca tgaaaattgt
     1861 gccggcatcg tccggcgtgg cggaactggt gaatctggga acccgcgaac acgcgggcct
     1921 gattcatgcg ttcagtgaac tgagcaatga tgttgatgta ttgctgatcg atacggccgc
     1981 cggcatttcc gacatggtga tgagttttgt caccgctgcc caggaaagcc tttttgttgt
     2041 caccaatgaa cccacgtcga ttaccgacgc ctatgccatg atcaagctgt tgaatcaaca
     2101 acatggcatg caccgcttca atattctggc aaacatggtg agaagccagc aggaagggcg
     2161 ggaactctat gcgaaattgt ccagggtcac cgaccggttt ctggatgtcg cgctacaatt
     2221 tatcggatgc attccctttg atgagtacgt gcgcaaggcc gtcatgcggc agaagccggt
     2281 ggtggaagcc tatccccgca gcaaggctgc gctggctttc cgtacactgg ccaacaaggt
     2341 cgacagctgg ccgttgccca cgacaccgcg cggacacctc gagtttttcg tggatcgcct
     2401 ggtgcaggga gctgcagtca gatagcaggg tgcaacatgc tgggtgtcaa ccgctacgct
     2461 atgtacagtg atgaatcggc accgcctctc gccgtcatgg tggaacagta cacgccgctg
     2521 gtgaaacgga tcgcccatca cctgctggcg cgcttgcctt cgtctgttgc ggtggaagat
     2581 ctctaccagg ccggcatgat cggcctgctc gatgccatca agcgttttga cagcaccaag
     2641 ggcgccagtt tcgaaaccta tgccggcatc cgcatccggg gtgccatggt ggacgaggtg
     2701 cgcaagggcg agtgggctcc ccgttctgtg caccgcaatg cccgtcgcat tcaggaagcc
     2761 atgcgcgctg tcgag
//
LOCUS       DASUEG010001028         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1871643, whole genome shotgun sequence.
ACCESSION   DASUEG010001028 DASUEG010000000
VERSION     DASUEG010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871643"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFX11_19320"
     CDS             <1..375
                     /locus_tag="VFX11_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX4940833.1"
                     /translation="ERFALVSLGVCSQTGEIPNCNIVLTVDGAEKRAQSEGAGPVDAT
                     FKAIEKIADSGTSLELYNVSAITSGTDSQGEVTVRLEKGGRIVNGHGADIDIVVASAK
                     AYLNALNHLAVAGKANPQTSGV"
     gene            379..1248
                     /locus_tag="VFX11_19325"
     CDS             379..1248
                     /locus_tag="VFX11_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940834.1"
                     /translation="MMQEAQRLAYLNALGITQYVPLAPIAGAPVLPVLQEADDPGYAP
                     EDLLVRESTSRYVDESMPVTVQPVAAPDFEAEEADTEKAVAGKTVTVPQQAFNDIPAA
                     SNIPAASDIPVLDLSKVKPEPAPVTAPAKPAAGLRFALAVVTLPQRLRLIVELAQPDA
                     PGFSAQEHRLVSDLLLALDAKTELNDSATKLFRWPLVNNPRIAADASATRDALFAFLA
                     GAQEAQAVPAIAFIGTRAVRSLHSAEPGSLFELPGISGRCLVTHSLKDIQQDWRLKPA
                     LWSHLHSLLSPAS"
     gene            1255..1713
                     /gene="rimI"
                     /locus_tag="VFX11_19330"
     CDS             1255..1713
                     /gene="rimI"
                     /locus_tag="VFX11_19330"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX4940835.1"
                     /translation="MQFQLMTEGLLPAVMDIEERAYRWPWTEGMFRDSLNSGYLCHVL
                     ADAGQLLGYTVIYVAVGESHILNICVEPDKQGLGIGRMLLRHALECAVDHGAEQAFLE
                     VRPSNLPAIGLYESMGFVQVGLRKEYYPAGDAREDALVYQADLAGFSRSK"
     gene            1875..>3091
                     /locus_tag="VFX11_19335"
     CDS             1875..>3091
                     /locus_tag="VFX11_19335"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX4940836.1"
                     /translation="MKLTTPTVGPIVGHTTPTETRLWFRGRFEGTKPKGYRRCFGAVR
                     WREAGKDWLGQKVGKMSPNFDMTGVFPLLKLKAATLYEYQAGWFLVDSELDSVQHSEF
                     NWDQLPLWRFKTPADTATAGHSVVVGSCRYLLKLFGGEIFDDRGDKTFHSVLVQHRSR
                     PIDALLMVGDQIYADDLNFISPDRKLDQFLERYQEVFSQDHVRRLMSEVPTYMILDDH
                     EIEDNWPNKATNKDMATLYPHAIHAYQIYQCSHSPVFELDKEGWVTGTPTRFWYTFSK
                     GCVDWFVMDVRSERLWHGDPARRQMIKSVQMNALLQWLSEGSDRVKMIVTSVPFIPDL
                     RTDADDKWGAYLAERSKILDHILANKIRRVAFVSGDVHCSFTSQITAPDDPQFRVYSI
                     ISSSLFWPYPHME"
BASE COUNT          600 a          946 c          929 g          616 t
ORIGIN      
        1 gaacgcttcg cgctggtgtc gctgggtgtg tgctcgcaga ccggcgaaat tcccaattgc
       61 aacatcgtgc tgaccgtgga tggcgccgaa aaacgcgcgc agagcgaagg ggccggcccg
      121 gtggatgcca ccttcaaggc catcgagaaa atcgccgaca gcggcaccag tctggagctg
      181 tacaacgtca gcgccatcac cagtggcacc gattcgcagg gcgaagtgac cgtgcgcctg
      241 gagaagggcg gtcgcatcgt gaatggccac ggtgccgaca tcgacatcgt ggtggcatcc
      301 gccaaggcgt acctgaacgc actcaatcat ctcgcggtgg ccggcaaggc caatccgcag
      361 accagcggtg tgtaaaccat gatgcaggaa gcgcagcggc tggcgtatct gaacgcactg
      421 ggcatcaccc agtatgtgcc gctggcgccc attgccggcg cgccggtgtt gccggtgctg
      481 caggaagcgg atgatcccgg ttatgcgccg gaggatctgc tggtgcggga atcgacgtcc
      541 cgctacgtcg atgagtcaat gccggtcacg gtacaaccgg tagccgcacc ggacttcgag
      601 gcagaagaag ccgataccga aaaagccgtt gccggaaaaa cggtgacggt tccgcagcag
      661 gctttcaatg acattcctgc tgccagtaac attcctgctg cgagtgacat tccggtgctg
      721 gatctcagca aggtcaaacc ggaaccagcc ccggtgacag cacccgccaa accggcggcc
      781 ggcctgcgtt tcgccctggc ggtcgttacc cttccccagc gcctgcgcct gatcgtggaa
      841 ctggcccagc cggatgcgcc gggtttttcc gcccaggagc accgtctggt cagcgatctg
      901 ctgctggccc tggatgccaa aacggaattg aatgacagcg ccaccaaact gttccgctgg
      961 ccgctggtga acaatccccg catcgccgcc gacgccagtg ctacccgcga cgccctgttc
     1021 gcgtttctcg ccggcgcgca ggaggcgcag gcagttcccg ccattgcctt cattggcact
     1081 cgcgctgtcc gcagtctgca cagtgccgaa ccgggatcgc tgtttgaact gcctggaatc
     1141 tccggtcgtt gtctggtcac ccacagtctg aaagacattc agcaggactg gcgcctgaag
     1201 ccggcgctgt ggtcccacct gcattccctg ctgtcgcccg catcctgatc cgccatgcaa
     1261 ttccagctga tgaccgaagg cctgctcccc gccgtgatgg atatcgagga gcgcgcctac
     1321 cgctggccct ggaccgaggg catgttccgc gacagcctca acagcggcta cctgtgccat
     1381 gtgctggccg atgctggcca gttgctgggt tacacggtga tttatgtggc ggtgggcgaa
     1441 agccacattc tgaacatctg tgtcgagcca gataaacagg gtctgggaat tggtcgcatg
     1501 ctgttgcggc atgccctgga gtgtgcggtg gaccacggcg cagaacaggc gtttctggaa
     1561 gtgcggccca gcaacctgcc cgcgatcggt ctgtatgagt cgatgggctt tgtgcaggtg
     1621 ggcttgcgca aggaatatta cccggccggt gacgcccgcg aggatgcgct ggtgtatcag
     1681 gcggatctgg ccggtttcag ccgctccaaa tagcgcccct ctctttccgc agttcgttac
     1741 gcacctttcc actttcttga ctatgtttct ctaaccgggt tgctggtgca ggcgcattcg
     1801 cgctgccctg gcgagatgga tctcggcgtt gcattgttca tgcgcatcaa gccacgcgaa
     1861 tcaaggagcg ccccatgaaa ctgactactc ccaccgtcgg accgattgtt ggtcatacca
     1921 ctcccaccga aacccgactc tggtttcgcg gccgtttcga aggtaccaaa cccaagggtt
     1981 accgccgctg ctttggtgct gtgcgctggc gcgaggcggg caaggactgg ctggggcaga
     2041 aggtgggcaa gatgtcaccc aatttcgaca tgaccggcgt gtttcccctg ctgaagctga
     2101 aagcggccac cctgtatgaa taccaggccg gctggtttct ggtggattcg gaactggaca
     2161 gcgtgcagca cagtgaattc aactgggatc agttgccgct ctggcgtttc aaaacgccgg
     2221 ccgacaccgc cacggccggt cattccgtgg tggtgggttc ctgccgctac ctgctgaaac
     2281 tgttcggcgg cgagattttc gatgatcgcg gcgacaaaac gtttcactcc gttctggtac
     2341 agcaccgtag ccggcctatt gacgcgctgc tgatggtggg tgaccagatc tacgcggacg
     2401 acctcaattt catctcgccg gaccgcaagc tggaccagtt cctggaacgc taccaggaag
     2461 tgttttccca ggatcacgtg cgccgcctga tgtccgaagt gcccacctac atgattctcg
     2521 acgatcatga aatcgaggat aactggccca acaaggccac caacaaggac atggccacgc
     2581 tgtaccccca tgccattcac gcctaccaga tttaccagtg cagccacagc cccgtattcg
     2641 agctcgacaa ggaaggctgg gtgaccggca cgcccacccg cttctggtac accttcagca
     2701 agggctgtgt ggactggttt gtgatggacg tgcgctccga acggctgtgg catggtgatc
     2761 cggcgcggcg gcaaatgatc aagtcggtgc agatgaacgc attgctgcag tggctgtcgg
     2821 agggctccga cagagtgaaa atgattgtca catcagtgcc cttcattccc gatctgcgca
     2881 cggatgccga cgacaagtgg ggtgcctatc tggcggaacg cagcaagatt ctggaccaca
     2941 tactggccaa caagattcgc cgtgtcgcct ttgtgtccgg cgacgtgcac tgttccttca
     3001 ccagccagat aacagcaccg gacgatccgc agttcagggt ctacagcatc atttcgtcgt
     3061 cactgttctg gccctacccg cacatggaga a
//
LOCUS       DASUEG010001029         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_502180, whole genome shotgun sequence.
ACCESSION   DASUEG010001029 DASUEG010000000
VERSION     DASUEG010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502180"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..536)
                     /locus_tag="VFX11_19340"
     CDS             complement(216..536)
                     /locus_tag="VFX11_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HEX4940837.1"
                     /translation="MKVLFLSAAILLAAGLGGCANVKGPSPGLLYSDYNGPVSLGSLD
                     KVTPAKSGKACAENILGAIATGDASIHTAKRNGGITRVASVEHHNTQYYGFYTRFCTV
                     VKGE"
     gene            complement(554..868)
                     /locus_tag="VFX11_19345"
     CDS             complement(554..868)
                     /locus_tag="VFX11_19345"
                     /inference="COORDINATES: protein motif:HMM:NF024546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HEX4940838.1"
                     /translation="MKLFQAATLALAAATLGGCAVVATPSHGLLYTKVQGPIVVGNGV
                     NTSKTGQACVTNILGLISTGDASIDAAKKAGGIKDVSSVDHDSTIMLGVYGQFCTVVK
                     GN"
     gene            complement(970..2697)
                     /locus_tag="VFX11_19350"
     CDS             complement(970..2697)
                     /locus_tag="VFX11_19350"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HEX4940839.1"
                     /translation="MALTTPALRVRAAQASRAGRKPENEDAMGIRLPTDHAQTRKGLA
                     MALADGVSGAGEGRRAAEVSVQGFLSDYYATPETWTVKTSAFRVLSALNRWLYGQGQQ
                     YTQIHQGFLTTFSALVLRSQTAHLFHVGDTRIQRLRDNTLEPLTLDHATRVGAEKTYL
                     TRAFGMDWQVDIDYRAVDVQSGDIFLLSTDGVHEFLPIPQLKKLLAQARDNPDACCEA
                     LLDAAYNAGSDDNISCQVLVIDSLDAPAADDSRLHATRLPLLSGLTIGQHIDGMLVEA
                     ELHASPRSQLYRVHDTITRRTMALKMPSPQLGDDAEHLSRFALEDWIGQRIDHPNVVK
                     GWPVPRQRQCLYLLQEYLEGETLSAWAEKNPRPPVQTVTSFAAQIIKGLRALHRRETL
                     HQDIKPDNLFLCSDGTLKLIDLGSARVAGLEDGTSHDRPGAAEYAAPEYALNLPRDAR
                     SDQFSLAITLYELLTGKHPYGETYARAVSPADFQKLSYTSACRYNPHVPLWLDAALKK
                     ACSLLMDERYAALGEFLVDLESPNPTLAPVSARPWLERDPAGFWRGLALVMSLIALAE
                     LGVIIFQAR"
     gene            complement(2708..4186)
                     /locus_tag="VFX11_19355"
     CDS             complement(2708..4186)
                     /locus_tag="VFX11_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HEX4940840.1"
                     /translation="MSSSKPTFPLFSFSEPRIRVLHFTWLAFFITFLIWFAHVSLMPM
                     IKESLHLTDAQVKAFLILNVALTIPARILIGILVDRYGPRASYSFLLAVCGMLCVGFA
                     LAQTFEQLAVMRFLLGLTGAGFVVGIRLVSEWFPAREVGIAEGIYGGWGNFGAAIAKI
                     GMPILAVTVFGGEFGWRYAIGLIGVIAIVYSVIFYRNVSDTPKGATYFKPKKAGGLEV
                     STKRDLVFYMLTNLPIYMILYVLAWRLAPSGLNIISQDFAYLCYAIITFLVVWQSWKI
                     WGVNKHLMHETVPVMQRYKFKQVAILNLAYLACFGSEIAIVSMLPMFFGDMFHLSPMA
                     MGLMAGSFTIMNLVARPGGGWLSDRIGRRKVLVACLAAQTVGYLLLSNVSESWGIVLA
                     VAVTLVTSIFVQAACGAVYSIVPLIQRRMTGQIAGMAGAYGNVGGVLFLTVLSFVSPH
                     LFFVVIAGVSAVAFLAANFIDEPKGHMVEVDEHGNVQMIAVE"
BASE COUNT          991 a         1313 c         1465 g          855 t
ORIGIN      
        1 ccagcagcag tggcgcaatc cctgcgaaaa gacggtgcat gggcttcatg acgagtcctg
       61 tagacagcaa aaacatgggg aaatgagcgg taaacccgcg agcggggccg attgtagcac
      121 cggctttccg ggggaatgtg ctgctttgtc gggcgattgt ggtcaggagg taaaaaacgg
      181 gctgtccccc atggcgggga gacagcccca ggcggtcact cgcccttcac cacggtgcag
      241 aaacgggtat agaacccgta gtactgcgtg ttgtggtgct ccacagaggc aacgcgtgtg
      301 atgcctccat tgcgcttggc cgtgtggatg gaggcatcgc ctgtggcaat ggctcccaga
      361 atgttttcgg cacaggcttt gccactcttg gcaggcgtga ccttgtccag cgagcccagc
      421 gagactggtc cgttgtagtc cgagtagagc agaccgggtg agggcccctt cacgttggca
      481 caacctccca agcccgccgc cagcaggatc gcggccgaca ggaacaagac tttcatgctg
      541 atgtccccgg ggctcagttg ccctttacca cagtgcagaa ctggccataa acgccaagca
      601 tgatggtgct gtcatggtcc acgctggaga cgtccttgat gccgcctgcc ttcttggcag
      661 cgtcgatgga agcgtcaccg gtggagatca ggccgaggat gttggtcaca caggcctggc
      721 cggtcttgga ggtgttaacg ccattgccga caacgatggg gccttgcacc ttggtgtaga
      781 gcaggccgtg gctgggggta gcaacgacag cgcaaccgcc cagagtggca gcggcaaggg
      841 cgagggtggc agcttgaaac agcttcatgg gatttccttg ttttccagtg ttgtcatgaa
      901 gccccttgag gggcgcgggc aacttaccgt gttgtcacgg agttgtcatg acttcagagg
      961 gtcaggagat caccttgcct gaaaaatgat cacaccgagt tcggcaagcg caattagcga
     1021 catcactagc gccagtccgc gccagaatcc ggccgggtct cgctccagcc agggacgggc
     1081 actgacggga gccagtgtgg ggttggggga ttccaggtcc accagaaact cgccgagcgc
     1141 ggcatagcgc tcatccatca gcaacgaaca ggcttttttc agcgctgcat ccagccacaa
     1201 ggggacgtgc gggttgtagc ggcaggcgct ggtgtagctg agcttctgga aatcggctgg
     1261 ggacaccgca cgggcatagg tttcgccgta aggatgtttg ccggtgagta gctcatagag
     1321 cgtgatcgcc agcgagaact ggtccgagcg cgcatcgcgt ggcagattga gggcatattc
     1381 cggtgcggca tactcggctg cccccggacg atcgtgcgat gttccatctt ccagccccgc
     1441 cacgcgggcc gacccgagat caatcagctt tagcgtgccg tcactgcaca ggaacagatt
     1501 gtctggtttg atgtcttgat gcagggtttc gcgccggtgc aatgcgcgca ggcccttgat
     1561 gatctgggcg gcaaagctgg tcacggtttg taccggaggg cgcggatttt tttcggccca
     1621 tgcggacagg gtttcccctt ccagatattc ctgcaacagg tacaggcact gacgctggcg
     1681 tggcaccggc cagcctttca ccacgttcgg atgatcaatg cgttgtccga tccagtcttc
     1741 cagcgcaaat cgagacaagt gctcggcatc gtcgcccagt tgcggtgacg gcattttcag
     1801 cgccatggtg cggcgtgtga tggtgtcatg cacgcgatac agctggctgc gcggactggc
     1861 atgcagctcg gcttccacca gcatgccatc gatgtgttgc ccaatcgtca ggccagacag
     1921 caacggcagg cgtgtcgcat gaaggcgact atcgtcggcg gcaggagcat cgaggctgtc
     1981 gatcaccagc acctgacagg aaatgttgtc atcgctgcca gcgttatagg ccgcatcgag
     2041 taatgcttcg cagcaggcgt cgggattgtc gcgcgcctgc gccaacagct ttttcagctg
     2101 cggaatgggc aggaactcat gcaccccatc ggtgctgagc aggaatatgt cgccggattg
     2161 cacatccacc gcgcggtagt cgatatctac ctgccagtcc atgccgaaag cgcgggtgag
     2221 gtaggttttt tcggcaccga cacgcgtggc gtgatccagc gtgagcggct ccagcgtgtt
     2281 gtcacgcaga cgctggatgc gggtgtcacc gacatgaaac aggtgcgcgg tctgcgaacg
     2341 cagaaccagt gcggaaaagg tggtgagaaa accctgatga atctgcgtgt actgctgccc
     2401 ctggccatac agccagcgat tgagggcaga cagcactcgg aaggccgatg tcttcaccgt
     2461 ccaggtttcc ggtgtggcgt aatagtctga caggaatcct tgtacgctga cttcggcagc
     2521 acgacggccc tcacccgcac cactcacccc gtcggcaagc gccatggcca gccccttgcg
     2581 agtctgggcg tggtcggtcg gcagacggat gcccatggca tcttcgtttt ccggcttgcg
     2641 acctgcccgg ctggcctggg cagcgcgtac gcgcagggct ggggtggtga gggccatgtt
     2701 cacggcatta ctccactgca atcatctgga cattgccatg ctcgtccact tccaccatat
     2761 ggcccttcgg ctcatcgatg aagttggccg ccaggaaggc aacggcggac acgccggcaa
     2821 tcaccacgaa gaagaggtgc gggctgacaa acgacagcac ggtcaggaac agcacgccac
     2881 ccacattacc gtatgcaccc gccataccgg caatctggcc ggtcatgcgg cgctggatca
     2941 gcggcacgat ggagtacacg gcaccgcagg ccgcctgcac gaagatggag gtaaccaggg
     3001 tgacggccac ggccagcacg atgccccagc tttcactcac gttcgacagc agcagatagc
     3061 ccacggtctg ggcagccaga caggccacca gcaccttgcg acgaccgata cggtcagaca
     3121 gccagccacc acccggacgt gcaacaaggt tcatgatggt gaacgagccg gccatcaggc
     3181 ccatggccat cggcgagagg tggaacatgt cgccgaagaa catcggcagc atcgacacga
     3241 tggcaatttc cgaaccaaaa caggcgaggt aggcaagatt caggatggcc acctgcttga
     3301 acttgtagcg ctgcatcacc ggcacggttt cgtgcatcag gtgcttgttc acgccccaga
     3361 ttttccacga ctgccacacg accaggaagg tgatgatggc gtagcagagg taggcaaaat
     3421 cctgcgaaat gatgttcagg ccggacggcg ccagacgcca tgccagtacg taaaggatca
     3481 tgtagatggg caggttggtg agcatgtaga acaccaggtc gcgcttggtc gaaacttcca
     3541 ggccgcccgc ctttttgggc ttgaagtaag tggcaccttt cggcgtgtcg ctcacgttgc
     3601 gatagaaaat caccgaatac acgatggcaa tcacgccaat caggccgatg gcatagcgcc
     3661 agccaaactc gccgccaaac acggtgacgg ccaggatggg catgccgatc ttggcgatgg
     3721 cggcaccaaa gttgccccag ccaccataaa tgccttcggc gatgccgact tcacgagccg
     3781 ggaaccattc gctcaccaga cggataccga ccacgaagcc ggcaccggtc agaccgagca
     3841 ggaagcgcat cacggcgagc tgttcaaagg tctgtgcgag cgcaaagccg acgcagagca
     3901 tgccgcagac ggccagcaaa aagctgtagc tggcacgcgg gccgtagcgg tcgaccagaa
     3961 tgccgatcag gatgcgcgcc ggaatggtca gcgcgacatt aaggataaga aaggccttta
     4021 cctgtgcatc ggtaaggtgc agcgattcct tgatcatggg catgagggag acatgcgcaa
     4081 accagatcag gaaggtgatg aaaaacgcca gccaggtgaa gtgcaggacg cgtatgcggg
     4141 gttcgctaaa tgaaaacagc ggaaacgtgg gcttgctcga agacatgatg aactccagac
     4201 gaaagcaggg ggaacagcgt tccatgtggc aggccttgcc ttcgccgggc gctccgttgc
     4261 tcccagcgac attgggagat gctctgcaat tgacatgcca gcagctgcaa aagcgtgcag
     4321 caccagggtt tcagttggtg cataccgttt cgggaggtct gctttggtgc gccaatcccg
     4381 gtcatggcac atgaagtgcg gcatttcggt gcacttgtgt ggtgccgtcg cagggcaggg
     4441 cggggtgaga ggaggggcag gggccttggc ggacgggaac tgcgagccat tccggtgcgg
     4501 gaagcaccaa gatggcatgg gcattgcaaa tacccaggca agcgtcccgc cttttactgg
     4561 atggttctac atgaaacggc aaaagctcat cgtggttggc aacggcatgg ccggcatccg
     4621 cacc
//
LOCUS       DASUEG010001030         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1231082, whole genome shotgun sequence.
ACCESSION   DASUEG010001030 DASUEG010000000
VERSION     DASUEG010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231082"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFX11_19360"
     CDS             complement(<1..211)
                     /locus_tag="VFX11_19360"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4940841.1"
                     /translation="MSEIRKAILYSVLNQYSLQIISFISVAVLARLLTPDQIGVFAVA
                     TSIAFLAIEIRSFGVSEFLVREKAVD"
     gene            complement(336..1298)
                     /locus_tag="VFX11_19365"
     CDS             complement(336..1298)
                     /locus_tag="VFX11_19365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4940842.1"
                     /translation="MQKTDWDLVSVVIPVFNAEAHLEETLASVLAQTYPNIEIIAVDD
                     GSKDRSAQILEKYRHRIRVVRQKNSGAAAARNLGVREAQGKWIAFLDADDLWSVDKVE
                     RQLAACGDRVWSYTNSMFLGGVNDGKQDSDLTEKYQGQVLEQLICNNFVGTSSVMVLR
                     QVFLDAGGFSESLQSIEDWEFWIRLASAHEICYVNEPLVRYRVHSSSASRSTRKTLPN
                     HMKVIETVFGVGGPAESLGHLKPSAKAQSYGICSQIAEEEGDFLFALRCSVLACTQAP
                     WALRRWERLLKSFVKYLISLIRFKFVQHSGWLAMVVVFEPVVPF"
     gene            1959..2885
                     /locus_tag="VFX11_19370"
     CDS             1959..2885
                     /locus_tag="VFX11_19370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4940843.1"
                     /translation="MQEPASDLVSVVIPVYNAEKYLEETLASVLAQTYPHIEIIAVDD
                     GSKDRSIQILEQYPDRIRVVRQANAGAAVARNRGVQEATGKWIAFLDADDLWTPDKIQ
                     RQLDASRGYVWSHTDSVFIGGVNDGRKDSDFTDKHQGQILEQLICSNFIGTSTLMIQR
                     QVFLDAGGFDESLRSIQDWELWIRIARQYPIGYVDAPLMKYRVHAVSASRSTRKTLPN
                     HLKVIEKVFAIGGPAETLSHLKSSAKARSYGICSQIAEEEGDFSFALRCSVLACQQEP
                     TEPSRWVRVTKAFIKYLLSLLGFRKNLSAGHH"
     gene            2920..>3917
                     /locus_tag="VFX11_19375"
     CDS             2920..>3917
                     /locus_tag="VFX11_19375"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4940844.1"
                     /translation="MSDLRKAILYSVLNQHSLQVISIVSIAILARLLSPDEVGIFAVA
                     TSIAFLATELRSFGVGEYLIREKEIDTGKIRTVLGVMVIMSWGLAVLLIAGAPWIAHF
                     YDNPDLRNLLWIIALPFFLAPHTAIPYALLVRDMKFTEILRINLLGSLVRNGGSIGLA
                     LLGFSYYSLAYGTLAGIVAEFLAISYFRPAGTPWMPAFSGLKHVFQVGMQISLSKFLQ
                     STSQNASDLILGRLATMKDVGMYSRGLGLILFLQSLLTNAVAPVALPHLSQVKRTGGS
                     VAEAYLYAVALVGAFALPLFAVVNIAAHPLINALFGDQWDVSVHVASNLAIWAMLQS"
BASE COUNT          924 a         1164 c          987 g          842 t
ORIGIN      
        1 ggtcaacggc cttttcccgc accagaaatt cgctgacgcc gaatgaacgg atttcaattg
       61 ccagaaacgc aatggaggta gccaccgcga acacaccgat ctgatccggc gtcagcaggc
      121 gcgccaacac agctactgaa ataaagctga tgatctgcaa cgaatactgg ttcagcaccg
      181 aatacagaat ggctttgcgg atttcactca tcgctgagca accgccaagg caggaaaaca
      241 aacagcaaga ggagaaagca ccgaaattga ctcatcgacg aggggtgaag tctttctcgg
      301 cagaaccagc atccacccgg tagcggtcgc acccctcaaa agggaaccac tggctcgaac
      361 actacgacca ttgccaacca gccggaatgc tgaacaaact tgaaccggat cagggatatc
      421 aggtatttga cgaatgactt gagcagacgc tcccagcgcc gcaacgccca cggcgcctgt
      481 gtacaggcca gaaccgagca gcgcagtgca aacaggaagt ccccttcttc ttcagcaatc
      541 tgcgagcaga tgccgtacga ctgcgccttg gcggacggct tcaaatggcc cagtgactcc
      601 gcaggcccac ccacgccaaa tacggtttca attaccttca tgtgattggg caaagtcttg
      661 cgcgtactgc gcgatgcaga tgaagagtgc acccgatatc gcaccagcgg ctcgttcaca
      721 tagcagatct cgtgagcact ggcgagccgg atccagaact cccagtcctc gatagactgc
      781 agtgactcac tgaaaccacc ggcatccaga aacacctgcc gcagcaccat caccgaggag
      841 gttcccacaa aattattgca aatcagctgc tccaggacct gaccctggta tttctcggtc
      901 aggtcactgt cctgcttgcc atcgttgacg ccacccagaa acatcgaatt tgtataagac
      961 catacgcgat ccccacaggc cgccagctga cgctcaacct tgtcgacact ccagaggtca
     1021 tcggcatcca ggaacgcgat ccacttgccc tgggcttcgc gcacacccag gttacgcgcg
     1081 gccgccgcgc cactgttctt ttgcctgaca acccggatac gatggcggta cttttccaga
     1141 atctgggccg accggtcctt cgaaccgtcg tccaccgcta tgatctcaat attcgggtag
     1201 gtctgggcca atacgcttgc cagcgtctct tccagatgcg cctcggcgtt gaacacaggt
     1261 ataacaacgc ttaccagatc ccaatcagtt ttttgcattc aaattcccat gcggacaatg
     1321 gcccactccc cagacagcag tcacaatgcc gatcatgggt atcccgtggc gaagaccgtg
     1381 ttgctggccc cataaacaaa agtacacgac actactgaat tggcaacatc ggcaccctgc
     1441 acgcgcaatc ccgagacatt aactcgcgga gaaaccgggt gtaacaggtt tatagtaaaa
     1501 ctcattccgt tattgactgt ccagcgatca ttttgtgatc atttacaata cgtctccggc
     1561 aggtttcgcc agccgcatcc actgtcaaag agttggatat gcttatggtg cagcgccatg
     1621 aaacttagta aattagtctc gctaaactgg ctctttcccg gccacctgcg ctcaccattt
     1681 tcacttgctt gaccgccaat gcttctggac gcagggaaga agccaaccaa tactgattta
     1741 ccggcttcca tcgcccgccc ctaacgttat tcgtttttcg gcaatccgcc ccaatccgac
     1801 gtatacgccg tagaggatag gcggaattcc tataaccacc gggcagaaga gacgtagatc
     1861 acacgcgcga gcaattattt aatgggcgcg acggaacttg aaaggcggat atgaatataa
     1921 tgttggcact tcgtttttgg tcataaaaag gctggtgaat gcaggaaccc gcatcggatc
     1981 tggtaagcgt tgtcatacct gtgtacaacg ccgaaaagta ccttgaagag acgctggcaa
     2041 gcgtattggc ccagacctac ccgcatattg agatcatagc ggtcgacgat ggttcaaaag
     2101 atcgctcaat tcagatactt gaacagtatc cagatcgtat acgcgtagtt cggcaggcca
     2161 acgcaggcgc cgctgtggcc cgtaatcggg gagttcagga agccaccggc aaatggatcg
     2221 cgttcctgga cgccgatgat ctgtggacac ccgacaagat ccagcgccaa ctggatgcca
     2281 gccggggata tgtctggtcc cataccgact cggtattcat tgggggcgta aatgatggca
     2341 ggaaagacag cgatttcacc gataagcacc agggccagat ccttgagcag ctgatctgca
     2401 gcaacttcat tggcacttcc accctgatga tccagcggca ggtgtttctg gatgccggtg
     2461 gcttcgacga atcactgcgt tctattcagg actgggaact ctggatccgg atagcgcgcc
     2521 agtatccgat cggatacgtg gatgcgccac tgatgaaata ccgtgtgcat gctgtttcgg
     2581 catcccgcag cacccgcaag accctgccca accatctgaa agtcattgaa aaggtgtttg
     2641 ccattggcgg accggcggaa acactgagcc atctcaagtc ctctgccaag gcgcgctcct
     2701 atggcatctg ctcccagatt gccgaggaag agggcgattt ttccttcgcc ctgcgctgct
     2761 ccgtgctagc ctgccagcag gaaccaaccg aacccagccg ctgggtgaga gtcaccaagg
     2821 cttttatcaa gtacctgctt tcgctgctcg gattcagaaa aaacctcagc gccggtcatc
     2881 actaggcaaa aacaaccctc tgcaattccc aaggcttcga tgagcgatct ccgcaaagct
     2941 atcctgtatt cagtgttgaa ccagcactcg ctgcaagtca tcagcattgt atcgatcgct
     3001 attctggccc ggctgctatc cccggacgaa gtcggcattt ttgccgtggc cacctcaata
     3061 gcgtttctgg caaccgagct gcgatcattt ggtgtggggg aatacctgat ccgggaaaag
     3121 gagatcgata caggaaaaat tcgcacggta ctcggtgtga tggtaatcat gtcctggggc
     3181 ttggccgtgc tgctaatcgc tggcgcgccc tggatcgccc atttctatga caacccggat
     3241 ctgcgtaacc tgctctggat tattgccctt cccttctttc tagcaccgca cacggccatt
     3301 ccctacgcgc tgctggtcag ggacatgaaa ttcacggaga tccttcgcat aaatcttctg
     3361 ggcagcctgg tccgcaacgg cggatctatt gggctggcac tactgggatt cagctactac
     3421 tccctggcgt atggcacact ggcgggtatt gtcgccgagt ttctggccat cagctatttt
     3481 cgcccggccg gcaccccctg gatgcccgcg ttcagcggtc tcaaacacgt attccaggta
     3541 ggcatgcaga tcagcctcag caaatttctg cagtcaacgt cgcagaacgc cagcgacctg
     3601 atcctggggc gcctggccac catgaaagat gtgggcatgt attcacgagg actgggcctt
     3661 atcctgttcc tgcaaagcct gctcaccaac gcggtggcac ccgttgcact gccgcacctt
     3721 tcacaggtca aacgcaccgg cggttcggta gcggaagcct acctgtatgc cgtcgctctc
     3781 gtcggcgcct ttgccctccc acttttcgct gtcgtaaaca tcgcggcaca ccctctgatc
     3841 aacgccttgt ttggagacca gtgggacgtg tcagtccatg tggcctcaaa cctggccatc
     3901 tgggccatgc tgcaatc
//
LOCUS       DASUEG010001031         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_126521, whole genome shotgun sequence.
ACCESSION   DASUEG010001031 DASUEG010000000
VERSION     DASUEG010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126521"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="ppsA"
                     /locus_tag="VFX11_19380"
     CDS             <1..1217
                     /gene="ppsA"
                     /locus_tag="VFX11_19380"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX4940845.1"
                     /translation="GDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAAIIARELGIP
                     AVVGCGDATDLLQEGQPVTVSCAEGDTGLVYEGKLGFDVQKNSIDSMPKLPFKIMMNV
                     GNPDRAFDFQSLPNEGIGLARLEFIINRMIGVHPKALLNYETLPKDVKQSVQRRISGY
                     AGPVEFYIEKLVEGVATLAASFYPKKVIVRLSDFKSNEYANLIGGKLYEPEEENPMLG
                     FRGASRYISDSFRDCFELECRALKKVREEMGLDNVEIMVPFVRTVGEAEQVIKLLEAN
                     GLKRGEKGLRVIMMCELPTNALLADDFLQHFDGFSIGSNDLTQLTLGLDRDSGIVSHL
                     FDERDPAVKALLKRAIEACNKAGKYVGICGQGPSDHPDLAKWLMEIGIDSISLNPDSV
                     LDTWFYLAENHA"
     gene            complement(1287..1718)
                     /gene="hisI"
                     /locus_tag="VFX11_19385"
     CDS             complement(1287..1718)
                     /gene="hisI"
                     /locus_tag="VFX11_19385"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX4940846.1"
                     /translation="MNIFKDNEHYSTGFQLDRDTLLAQVKYNNDGLVPAIAQQYDTKE
                     VLMLAWMNAAALQETLQTGRVCYWSRSRNAFWRKGESSGQVQILKSARIDCDGDTLLL
                     DVDQTGPACHTGRRHCFFYKFEGDHVVIDGDVMIPPDELYK"
     gene            1811..2728
                     /locus_tag="VFX11_19390"
     CDS             1811..2728
                     /locus_tag="VFX11_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4940847.1"
                     /translation="MQNQPDHIVVMFADVSGSTQLYERLGDTMAHECIAESLQRIIRH
                     VKQNRGRLIETIGDEAMVTFAAPLDAIHAACAIQRQFAAEPVARGQRVKVRIGFHFGP
                     IEYDANQHPFGDTVNVAARVVALCEAGRIIATRDSLQHINSAGEFTLRSYQRTRVKGK
                     SEPLVTDEIVWDNEDSTSLFNATQNTQVKIAGPMMYLVYRNQPIALGTDRVSFVVGRG
                     PECDLVIDNALASRAHARLEIRWGEVYLVDHSTNGTYLRLGEGRRESNGGSVRLHRRE
                     LLLTGKGEIGIGTPVENAGESCLIQFEIY"
     gene            complement(2771..3013)
                     /locus_tag="VFX11_19395"
     CDS             complement(2771..3013)
                     /locus_tag="VFX11_19395"
                     /inference="COORDINATES: protein motif:HMM:NF045308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940848.1"
                     /translation="MTQDLQAKRKELADKREQLLQRLDAIKRDIGGGLSADFEEQAVQ
                     LENADVLAEISRVTNEELQKVTQALQRIEQELARKH"
BASE COUNT          662 a          926 c          988 g          674 t
ORIGIN      
        1 tgggagatgt tctcgtcact gacatgaccg atcccgactg ggaaccgatc atgaagcgcg
       61 cttccgccat cgtgaccaat cgtggtggcc ggacctgcca cgctgcgatt atcgcgcgcg
      121 aactgggcat tcctgctgtc gtgggctgcg gcgacgccac cgacctgctg caggaaggcc
      181 agcccgtaac cgtgtcctgc gccgagggcg acaccggtct ggtatacgag ggcaagctgg
      241 gcttcgatgt gcagaaaaac tccattgatt ccatgcccaa gctgccgttc aagatcatga
      301 tgaacgtggg caacccggac cgggcattcg acttccagtc cctgcccaat gaaggcatcg
      361 gcctggcccg tctggagttc atcatcaacc gcatgattgg cgtgcatccc aaggcgctgc
      421 tgaactacga aaccctgccc aaggacgtca agcagagcgt gcagcgccgt atctccggct
      481 acgctggtcc ggtggagttc tacatcgaga agctggtgga gggtgtagcc accctggcgg
      541 cgtctttcta tcccaagaaa gtgatcgtgc gcctgtctga cttcaaatcc aacgagtacg
      601 caaacctgat cggcggcaag ctgtatgagc ctgaggaaga gaacccgatg ctgggcttcc
      661 gtggtgcgtc ccgctacatc tccgacagct tccgcgactg tttcgagctg gaatgccgcg
      721 cgctgaagaa ggtgcgcgag gaaatgggtc tggacaatgt ggagatcatg gtgccgttcg
      781 tgcggaccgt gggcgaggca gaacaggtga tcaagctgct ggaggccaat ggcctgaaac
      841 ggggtgagaa gggcctgcgc gtgatcatga tgtgcgagct gcccaccaac gcgttgctgg
      901 cggacgattt cctgcaacac ttcgacggct tctccatcgg ctccaacgac ctgacccagc
      961 tgaccctggg cctggaccgg gattccggca tcgtgtccca tctgtttgat gaacgcgacc
     1021 cggccgtgaa agcattgctg aagcgcgcca tcgaagcctg caacaaggct ggcaagtacg
     1081 tgggtatctg cggccagggg ccgtcggacc acccggatct ggccaagtgg ctgatggaaa
     1141 tcgggatcga cagcatctcg ctgaaccccg attccgtgct ggacacctgg ttctatctgg
     1201 cggaaaacca cgcctgatca gataccggcg gaagaagggg gctacggccc ccttttttgc
     1261 gtctgaaggg aagggtgctg gacggcttac ttgtagagtt catccggcgg gatcatcacg
     1321 tcgccgtcga tgacgacatg gtcaccttcg aacttgtaaa agaaacagtg gcgccggccg
     1381 gtatgacagg cggggccggt ctggtccacg tccagcagca gggtgtcgcc atcgcagtcg
     1441 atgcgggcag atttcaggat ctgcacctga ccgctgcttt cacccttgcg ccagaaggcg
     1501 ttgcgggagc gggaccagta acagacccgc cccgtctgca gggtttcctg cagggcagcg
     1561 gcgttcatcc aggccagcat cagcacttct ttggtgtcat attgctgcgc aatcgccgga
     1621 acgagtccat cattgttata tttcacctgg gccagcagcg tgtcgcgatc cagctgaaag
     1681 ccggtagaat aatgttcgtt gtctttgaaa atgttcatgg caatgaaggg gtcatgctga
     1741 gttcgaggtt tcagtctcgt gtacggcgtg accccgttgg gtaccaccag cacgaatcag
     1801 ggatattttc ttgcaaaatc aaccggatca catagtagtc atgttcgccg acgtatccgg
     1861 cagcacccag ttgtatgagc ggctcgggga tacgatggcg catgagtgta tcgcggaaag
     1921 cctgcagcgt atcatccggc atgtcaaaca aaaccggggg aggctgatcg aaaccatcgg
     1981 cgacgaggcg atggtcacct tcgcagcgcc cctggatgcc attcatgccg cgtgcgcgat
     2041 ccagcgtcag ttcgcagcgg agccggtggc gcgcgggcag cgggtgaaag tgcggatcgg
     2101 gtttcatttt ggccccattg aatatgacgc caaccagcat ccctttggcg acactgtcaa
     2161 cgtggctgcg cgggtggtgg ccctgtgcga ggcaggccgc atcattgcca cacgcgacag
     2221 cctgcagcac atcaacagcg cgggcgagtt tacgttgcgc tcataccagc gcacacgcgt
     2281 gaagggcaaa agcgaaccgc tggtgaccga cgagattgtg tgggacaacg aggattccac
     2341 ttcgttgttc aacgccaccc agaataccca ggtgaagatc gctggcccca tgatgtacct
     2401 ggtctatcgc aaccagccta ttgcactggg tactgaccgg gtcagctttg ttgttgggcg
     2461 tgggccggaa tgcgatctgg tgatcgacaa tgcattggcg tcgcgggccc acgcacggct
     2521 ggagattcgc tggggagaag tctacctggt ggatcacagc accaacggca cttatctgcg
     2581 gctgggtgaa ggccggcgcg aatcgaacgg aggttccgtg cgcctgcacc ggcgcgaatt
     2641 gctgttgaca gggaagggcg agatcggcat cggcacaccg gtggaaaatg ccggggaaag
     2701 ctgcctgatc cagtttgaaa tctactgagg cgcggacata tcgctctttt gcgcctcaac
     2761 ctttcacgcc tcagtgcttg cgcgcgagtt cctgttcgat tcgttgcagt gcctgcgtca
     2821 ctttctgcag ttcctcattg gtcacccggc tgatttccgc cagcacatcc gcgttttcca
     2881 gttgcaccgc ctgttcttca aagtcagctg acagaccacc gccaatatcg cgcttgatgg
     2941 catccaggcg ttgcagcagt tgctcgcgct tgtctgccag ttcttttctc tttgcctgca
     3001 aatcctgggt catgccagtg ttctccgata agtattgaaa aattatccct tgtcggggcc
     3061 aatgggttat tgatgaatat caagtctcgg agaggcagcg aaactggcca ggccaatcgc
     3121 ggtaattgaa tacctgcccg gtgtctcgca cgttggcgat gcgggacaga tcgagatcgg
     3181 ccaacaacca ctgaggcgtg ccgaattcgc ccagcgccag cacaccgtta tcgggaaagc
     3241 cccggtctac
//
LOCUS       DASUEG010001032         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1784335, whole genome shotgun sequence.
ACCESSION   DASUEG010001032 DASUEG010000000
VERSION     DASUEG010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784335"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /gene="hypF"
                     /locus_tag="VFX11_19400"
     CDS             complement(<1..2041)
                     /gene="hypF"
                     /locus_tag="VFX11_19400"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX4940849.1"
                     /translation="MMATDAGRPETWALHLTLRGEVQGIGLRPRVKRWADALGLRGWI
                     RNQGDQVALQVMGPASAVQQMLDQLRGLDCAVDCAELDQARLSAERVAQSGFAILPGE
                     PLRADRLYQVPADRGICAQCLREFHDPADRRFHYPFISCNDCGPRFSILTALPFARAN
                     TAYSRLPPCLDCQAEFEDPENRRFHSELISCAACGPQLSGVGLSGIGVPAPAAPDDLI
                     QHALDRLNGGGIVGLKSLSGMQLLACADNDSAVRRLRERKQRPFKPLALLVPSLVWAQ
                     RLVQVNDVAAQWLVSPQRPIVLLPKNTAADALLAASVAPDVPDLGLMLPVTALQSWLV
                     EAVGAPLVCTSANVSDEPILFANADLETAVGTLCDFALLHDLEIEFPQDDSVLMENQG
                     RTTLLRRARGFCHTVFPVAPGEATLALGGDLKNTFALARGGGAVLGPHLGDLASVKTL
                     QRRDWLLERFAALQHFTPQHICADAHPGYISRQQAQRMAQQSEPHRLQRVQHHRAHLL
                     ANQIACPLPPRYLALIWDGNGYGDDGTLWGSEAFLVDGGQVTRVAALAPFALPGGVQA
                     LLQPWRLALSLSFAAGISRADALPRLVSGSLPESQLAAVWQLLEKNLNCPRSCAMGRL
                     FDGMSYLLLGTERNCYEGYAPVQLQYSAQRGLDDSAAFPFDGQGDGRVGVVAPFAI"
     gene            2183..>2425
                     /locus_tag="VFX11_19405"
     CDS             2183..>2425
                     /locus_tag="VFX11_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940850.1"
                     /translation="MTSSSTASGSGLPRLFSRCSPLPQWDAAAAQVLLQARFGRPWQR
                     QDLLQALIVIQHHFGAITVPAQTWLARHCNTNTADVR"
BASE COUNT          491 a          820 c          705 g          409 t
ORIGIN      
        1 ggattgcgaa tggagcgacc actccgactc gcccgtcgcc ttgcccatcg aatggaaatg
       61 cggcggaatc atccagcccg cgctgcgcac tgtactgcaa ttgcacgggc gcataaccct
      121 cgtagcaatt gcgttccgtg cccagcagca ggtaactcat cccgtcaaac agtcggccca
      181 tggcacagct gcggggacag ttgagattct tttccagcaa ctgccagacg gcggccagtt
      241 gcgattcagg caacgagccg gaaaccagtc gcggcaacgc gtccgcacgg gagatgccgg
      301 cggcaaaact cagggacaac gccaaacgcc agggttgcag cagcgcctgc actccacccg
      361 gcaacgcaaa tggcgccaat gctgcgacac gggtaacctg tccaccgtcc accagaaacg
      421 cttcgctgcc ccacagggtg ccgtcgtcac cgtagccgtt gccatcccag atcagcgcca
      481 ggtagcgcgg cggcagcgga caagcaatct gattggccag caggtgggca cggtgatgct
      541 gcaccctttg cagcctgtgt ggttcggatt gctgagccat gcgctgcgcc tgctgccggc
      601 tgatataacc cggatgggca tccgcacaaa tgtgttgcgg tgtgaaatgc tgcagcgccg
      661 caaaacgttc cagcaaccag tcccgtcgct gcagggtttt taccgatgcc agatccccca
      721 gatgggggcc cagcactgcc ccgccacccc gcgccagcgc gaaggtgttt ttcagatcac
      781 cgcccaatgc cagggttgcc tcgcccgggg caacaggaaa aacggtatgg cagaaacctc
      841 gggcacgccg caacaatgtc gtgcggccct ggttttccat cagcacggaa tcgtcctggg
      901 gaaattcgat ctccagatca tgcagcagcg cgaaatcgca cagggttcca acggctgttt
      961 ccagatccgc gttggcaaac aggatcggct cgtcgctgac attggcgctg gtgcagacca
     1021 gcggcgcacc gaccgcttcc accagccatg actgcagcgc ggtcacgggc aacatcaggc
     1081 ccagatccgg cacatccggt gccacggacg ctgccagcaa tgcatccgca gccgtatttt
     1141 ttggcaacag cacgatgggg cgttgcggcg acaccagcca ctgcgcggcc acgtcgttca
     1201 cctgcaccaa ccgctgcgcc cagaccagcg atggcaccag caacgccagc ggtttgaacg
     1261 ggcgctgctt gcgttcacgc agccggcgca cggcggagtc gttatcggcg caagccagca
     1321 gctgcattcc ggacagggat ttcaggccga caatgccgcc gccattcagg cgatccagcg
     1381 catgctggat cagatcgtca ggtgctgccg gcgctggcac accaatacca gacaagccaa
     1441 cgccagacag ctgcggcccg cacgccgcgc aactgatcag ctcgctgtga aagcgccggt
     1501 tttccggatc ttcgaactcc gcctggcagt caaggcacgg cggcaggcgc gaatatgcgg
     1561 tattggcgcg cgcgaatggc agcgctgtca gaatgctgaa acggggccca caatcattgc
     1621 aactgataaa ggggtaatga aaacgacgat cagcaggatc gtgaaattcc cgcagacact
     1681 gcgcacagat accgcgatcg gccggcacct gatacagccg gtcggcgcgc aacggctcgc
     1741 cgggcaggat cgcaaaaccg gattgcgcca cccgttcagc cgacaaccgg gcctgatcca
     1801 gttcagcaca atcaacagcg cagtccagcc cgcgcaactg gtccagcatc tgttgcacag
     1861 ccgacgccgg gcccatcacc tgcagcgcca cctggtcgcc ctggttgcga atccagcccc
     1921 gcaggcccag cgcgtctgcc cagcgtttca cccggggccg caggccgatc ccctgcactt
     1981 cgccgcgcaa ggtcaggtgc agcgcccagg tttccggccg gccggcatcc gttgccatca
     2041 tgctgtcacc ctaatgatgt ttatcagatt cggttgtccc cacgtttgac ttctttgatc
     2101 tgcatttgca cccgctggat gggatcagta taagatagga aaccatcgaa cctgacagta
     2161 acttgtggcc acggcgaccg acatgacgtc atcctccacc gcttctggca gcggcttgcc
     2221 ccgcctgttt tcccgctgct ctcctctgcc gcagtgggat gcggcggccg cgcaggtatt
     2281 gctgcaggcc cgcttcggcc gcccgtggca acgacaggat ctgctgcagg cgctgatcgt
     2341 catccagcat catttcggcg ccatcaccgt gccggcgcaa acctggctgg cgcgccattg
     2401 caacaccaac acggccgatg tccgc
//
LOCUS       DASUEG010001033         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_303204, whole genome shotgun sequence.
ACCESSION   DASUEG010001033 DASUEG010000000
VERSION     DASUEG010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303204"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1359)
                     /locus_tag="VFX11_19410"
     CDS             complement(115..1359)
                     /locus_tag="VFX11_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4940851.1"
                     /translation="MTQEQQVCIIIGASHAGAQLATSLRKEGWAGRIVVIGDETQLPY
                     HRPPLSKALLMKEKTPDQIEIFKATVYEKSSVEFRLGLSVEKIDRAAKTVLLNNGETL
                     AYTKLALCTGARVRKLEIPGANLKGVHYLRTLADAEKIQSEVKPGGHAVIVGGGYIGL
                     ETAASLRKLGMDVTVLEMMNRVLERVTAPELSAYYTDLHQRHGVKIITNAQALELRGQ
                     DRVQQVVCNEGRVLGADLVIIGIGVIPNTELAADAGLEISNGVVVDEFAQTSDPDIVA
                     AGDCTNHPNDLLGYRLRLESVPNAMEQGKAAAASICGLKKAYHALPWFWSDQYNAKLQ
                     IAGMNRGYERVIIRGVPSNDQFVAWYMKGDQVLAADCINSAKEFMAAKKIIAQKLVLK
                     ESDLADAGVDLAAVVNGMGAGA"
     gene            1577..1897
                     /locus_tag="VFX11_19415"
     CDS             1577..1897
                     /locus_tag="VFX11_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4940852.1"
                     /translation="MPNIQVTDLSGKVSTLKAKSGETLMEALRDNGYSDIEAVCGGVC
                     SCSTCHVYLEGDWFSKLGERSEDENQLVTSTDHFKPNSRLSCQVTVSDDMDGMAITIA
                     HQTY"
     gene            complement(2080..>3970)
                     /locus_tag="VFX11_19420"
     CDS             complement(2080..>3970)
                     /locus_tag="VFX11_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940853.1"
                     /translation="DATPLPVDPRTLVQPGISDGKAVHFNAYWKECHVDPALVKEAGH
                     PETCGELRDVFYRGETLMDTGSEGVAALFAGTRYNALSAAAGISTFSASQYNKLWTIW
                     GGYSQRPADFDKLVSNRYGSGMHTERNPYPLPGEDPNQTNGGSGQLPEMFTQVRKADG
                     SWSGRIGVTCHGCHSGEVGNKQQGEDLGFIFGGSSATDLNLFLRDMLPLGYLASGVTP
                     LNLTQTRGTNNASAVNIAFLFPQNGLPSISGFLKILGSGSTGSMDSPPFWNMGHRPLK
                     FVDGLFPMDAPRVDAVFYTPFFGLFGGTSGGLGKEGMAWMRQHGPDMNTWVETLKAPR
                     YPYPVDTALAEAGSVLFHELDLWAPERNNPIDRPEGNGSCAACHGAYAPRYVNDPEFL
                     ATPELEGMAGYIVPTRIIGTDKRRVATNTEGMQVTGSKNFFGYPETKGTENDCGPQGQ
                     KRLRGDREMGYLAQPLYGVWASAPYFHNGSVPNVWEVLKPADRKPIWRRVSAPRLEGA
                     EGEVVMGFDTDLDRAFDPQKLGWKYDEIPCVNLPPMVKPGFNCSTKNIYVTPWPQRLL
                     EGLYGSLTGAWNVDFPYIRTTENMENRKIYNTHMYSQGNEGHEFTSVLTDAERTAIIE
                     YLKTL"
BASE COUNT          718 a         1200 c         1183 g          869 t
ORIGIN      
        1 ggatggccgc tgtcgagcct acagggatgt acttgtggcg tgtcccgaac cccaacgcca
       61 gaccctcgat cgcaggaacg tcaggtccgg cgcgcattcc aatttcaaaa caacttacgc
      121 cccagccccc atcccgttca ccaccgccgc cagatccaca cccgcatccg ccagatcgct
      181 ttccttcagc accagcttct gcgcgatgat cttcttcgcc gccatgaatt ccttcgcgct
      241 gttgatgcag tccgccgcca gtacctgatc acctttcatg taccacgcca cgaactgatc
      301 attggacggc acgccgcgaa taatcacccg ctcgtaacca cgattcatgc cggcgatctg
      361 caacttcgcg ttgtactggt ccgaccagaa ccagggcagt gcatgatagg cttttttcaa
      421 gccgcagatc gaagcggccg ccgcttttcc ctgctccatg gcgttgggca cggattccag
      481 ccgcagacga tagcccagca gatcgttggg gtggttggtg cagtcgcctg ccgccacgat
      541 gtccgggtcg gaggtttgcg cgaattcatc cactaccacg ccgttgctga tttccagccc
      601 ggcgtccgcc gccagttcgg tgttgggaat cacgccaatg ccgatgatca ccagatccgc
      661 gcccagcacg cggccctcgt tgcacacgac ctgctgcaca cgatcctggc cacgcaattc
      721 aagcgcctgc gcgttggtga tgatcttcac gccgtggcgc tggtgcaggt cggtgtagta
      781 ggccgacagt tccggcgccg taacccgttc cagcacccgg ttcatcattt ccagcacggt
      841 cacgtccatg cccagcttgc gcagcgatgc cgccgtttcc agcccgatat aaccgccacc
      901 cacgatcacc gcatggccgc cgggtttcac ctcgctctgg attttttctg catccgccag
      961 ggtgcgcagg tagtgcacgc ctttcaggtt ggcgccggga atctccagct tgcgcacgcg
     1021 ggcgcccgtg cacagggcca gtttggtgta ggccagggtt tcgccgttgt tgagcagcac
     1081 ggttttggca gcacggtcga tcttttccac gctcaggccc agcctgaatt ccacgctgga
     1141 tttttcatac acggtggctt tgaaaatctc gatctggtcg ggcgtctttt ccttcatcag
     1201 cagcgccttc gacagcggcg ggcggtgata gggcagctgg gtttcgtcgc cgatcacgac
     1261 gatgcggccg gcccagcctt ccttgcgcag actggtggcc agttgggcgc cggcatggct
     1321 ggcgccgatg atgatgcaaa cctgttgttc ctgcgtcatg acttcttttc ccctgcagtc
     1381 gaaatgtcgt gctgttgcct attgtacctg caatggcagg cttgattttg tctgccataa
     1441 agtatacggt gtatactccg gaagcaatag taaatccgct tttttcctgt gccaatctcg
     1501 gccctgtgcc ggccattcac cgggaaatcg aacaaaaaca atttcatatt caataagaat
     1561 tgcgggagta agcaccatgc caaacattca agtcaccgat ctgtcgggaa aggtatccac
     1621 gctgaaggcc aagagcggcg aaaccctgat ggaggcgctg cgggataatg gttacagcga
     1681 tatcgaagcc gtatgcggtg gtgtctgttc ctgttccacc tgtcatgtgt acctggaagg
     1741 cgactggttc agcaaactgg gtgagcgcag cgaagacgag aaccagctgg tgaccagcac
     1801 cgatcacttc aaacccaatt cccgcctgtc gtgccaggtc acggtgtccg acgacatgga
     1861 cggcatggcc atcaccatcg cccaccagac ctactgaggt ttcagggtgt ccgcgcttgc
     1921 gcaggcggcc aggcgccccg gtgtgagcat gaacgacatc accagtcacc agcgggtgga
     1981 cagcccgagc tgttttctac ccattgtttt ctgcaattca gtgtctgcaa aaaaaaaggg
     2041 caacggcgtt aaccgttgcc cttttctact gctgccttct caaagcgttt tcaggtactc
     2101 gatgatggcg gtgcgttcgg cgtcggtgag tacgctggtg aactcgtggc cctcattgcc
     2161 ctggctgtac atgtgcgtgt tgtagatctt gcggttctcc atgttttccg tggtgcggat
     2221 ataggggaaa tccacgttcc aggcgccggt caggctgccg taaagtcctt ccagtaaccg
     2281 ctgcggccag ggtgtgacgt aaatgttctt cgtgctgcag ttgaaaccgg gcttcaccat
     2341 gggtggcagg ttcacgcagg gaatctcgtc atatttccag cccagtttct gcgggtcgaa
     2401 ggcgcggtcc agatcggtat cgaagcccat gaccacttca ccttcagcac cttccagtcg
     2461 cggtgccgaa acgcggcgcc agatgggttt acgatccgcc ggtttcagca cttcccagac
     2521 attgggaacc gagccgttgt ggaagtaggg tgccgacgcc cacacgccat acagcggttg
     2581 tgccagatag cccatctcac ggtcgccgcg caggcgtttc tggccttgcg ggccgcagtc
     2641 gttttcggtg cccttggttt ccgggtagcc aaagaagttt ttggaaccgg tcacctgcat
     2701 gccttcggtg ttggtggcga cacggcgttt gtcggtgccg atgatgcggg taggtacgat
     2761 gtagccggcc atgccttcca gttccggcgt tgccaggaat tccggatcat ttacgtagcg
     2821 cggtgcatag gcgccgtggc aggccgcaca ggagccgttg ccctccggcc tgtcgatcgg
     2881 gttgttgcgc tccggtgccc acagatccag ttcatggaac aggacggatc cggcttccgc
     2941 gagtgcggtg tcgaccgggt aggggtagcg cggggctttc agggtttcca cccaggtgtt
     3001 catgtccgga ccgtgctggc gcatccaggc catgccctct ttgcccaggc cgccggaggt
     3061 gccgccaaac aggccgaaga agggcgtgta gaacacggca tccacccgcg gggcgtccat
     3121 cgggaacagg ccatcgacga atttcagcgg gcggtggccc atgttccaga agggcggtga
     3181 atccatgctg ccggtggaac cggagccgag gattttcaga aagccgctga tgctgggcag
     3241 accgttctgt ggaaacagga aggcgatatt cacggcgctg gcgttgttgg tgccgcgggt
     3301 ctgcgtcagg ttcagtggtg taacgcccga ggccagataa cccagcggca gcatgtcacg
     3361 caggaacagg ttcaggtcgg tggcgctgct gccgccgaat atgaagccca ggtcttcgcc
     3421 ttgttgcttg ttgcccactt cgccgctgtg gcagccatga caggtaacgc cgatccggcc
     3481 gctccagctg ccgtctgcct tgcgcacctg ggtgaacatt tccggcaact ggccgctgcc
     3541 gccgttggtc tggttgggat cttcgcccgg caggggatag ggattgcgtt cagtgtgcat
     3601 gccggagccg tagcgattgg acaccagttt gtcgaaatcc gccgggcgct ggctgtagcc
     3661 gccccagatg gtccagagtt tgttgtattg cgaagcactg aacgtggaga tgcccgcggc
     3721 agcagacagg gcgttgtagc gggtgccggc aaacagtgcc gccacgcctt cactacccgt
     3781 gtccatcagg gtttcgccac ggtagaaaac gtcccgcagc tcgccgcagg tctccgggtg
     3841 gcccgcttct ttcaccagcg ccggatcgac gtgacattct ttccagtacg cgttgaagtg
     3901 aaccgctttg ccgtcactga taccgggctg caccagtgtg cgcggatcga caggcagagg
     3961 cgttgcatcg
//
LOCUS       DASUEG010001034         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_463859, whole genome shotgun sequence.
ACCESSION   DASUEG010001034 DASUEG010000000
VERSION     DASUEG010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463859"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="dbpA"
                     /locus_tag="VFX11_19425"
     CDS             complement(<1..1007)
                     /gene="dbpA"
                     /locus_tag="VFX11_19425"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004713123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEX4940854.1"
                     /translation="MSSVPESPAFSTQSLPEALLDTLNELGYHAMTPIQAQSLPSLLA
                     GRDVIAQSKTGSGKTAAFGIGVLSALNPRDFSVQALVLCPTRELADQVLKEMRRLARF
                     AGNIKMLSLCGGVPMGPQLDSLEHGAHVIVGTPGRVLKHLAKGSLDLGKLKVLVLDEA
                     DRMLDMGFHDSIVEVVDQMPSKRQTLLFSATYSKEVRALSTRFQRDPVSVTVADAPEA
                     VKIAQIFHEVDNRERVNAVAAVLRHHKPESAVLFCNTKQRCEEVVTALSEKGFQVAAL
                     HGDLEQRDRDLVLVKFSNRSCPLLVATDVAARGLDIKDLAMVINVEMAFDPEVHVHRI
                     GR"
     gene            complement(1115..1969)
                     /locus_tag="VFX11_19430"
     CDS             complement(1115..1969)
                     /locus_tag="VFX11_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HEX4940855.1"
                     /translation="MKTPKRLEPLLEDGLIDEVVAQLKSGKEATVYLVRCGGVLRCAK
                     VYKDAHHRSFKQASTYREGRKVRNTRDARAMEKGSRFGKQELEEAWQNAEVSALYRLA
                     DAGVRVPTPHGCFDGVLLMDMVSDGHGRPAPRLSEVSFTPEEAVACHHQLVHEVVRML
                     CAGLVHGDLSEYNILIDPKGPVIIDLPQAVDAAGNNNAFTMLERDVQNLADYFGQFAP
                     ELLDTRYAKEIWALYESGELTPESHLTGEFEEDETPADLDSLLREIMDARDEEERRKA
                     AEEQGDEF"
     gene            complement(1980..2999)
                     /gene="nagZ"
                     /locus_tag="VFX11_19435"
     CDS             complement(1980..2999)
                     /gene="nagZ"
                     /locus_tag="VFX11_19435"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX4940856.1"
                     /translation="MAIGALMLDVEGLTLTDDEKKLLAHPHVGGLILFARNFQSVEQV
                     TTLVQEIRAVRPEILIAVDQEGGRVQRFRDGFVRLPPMKVFGDLYAENPGEARQQAYQ
                     CGWLMASEVLAVGVDISFAPILDVDCGISQVIGDRAFNADPQVAVTLLREFIRGMHEA
                     GMAATGKHFPGHGSVEADSHVALPVDERPWAEIEAHDLVPFAALSRELQGIMPAHVIY
                     SKVDPNPAGFSSFWLQDILRERLGFDGVIFSDDLSMEGAVATGSFSDRANAALDAGCD
                     RVLVCNHRAGALEVLEALEKRGQFPEQRRFEAMKGKFRHSWTSLHDDDNWKMVASRVG
                     VNTVA"
     gene            complement(3015..>5573)
                     /locus_tag="VFX11_19440"
     CDS             complement(3015..>5573)
                     /locus_tag="VFX11_19440"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX4940857.1"
                     /translation="LPDLVMRRRFSLLAIPLMVLALSGCDAMDLMLRNTVDKAEGVVQ
                     LKGVSAPVTIRRDAFGVPLVEAASEDDLAFGLGYVMASDRLAQMVSYSLVAQGRLSEM
                     AGATTRDLDIYMRTLGLHRHAEQHLKLASPALRHKLQRFADGVNAWMASHQDRLPLDF
                     KLSAYAPEAWTPLHSMDVFMMLNLGLSLNLPEEVAFLDLARVVGPEKAAWLIPSYPDE
                     PVALDEAAKLGDFPFKDLQTNVVAQSRVSQQLASLFVPVRQAASNNWAVAAARTKGKA
                     SILANDTHLMLEHPPVWMLVQVSAPGYRAGGIAIAGLPGVVAGYNGHVAWGMTMVMAD
                     SQDVFVEKLKQENGKTFYLYRGEWQPVTERREVIRIKGEPDHVVTVQETVHGPLLNDA
                     LRGRRVNEVMLPTQRVSGGYGLAVQTTAGVADLSMERFFRLGQAADFAAVGEAIQGIG
                     AIHLNLLFADAANIGWQVTGLYPQRRKGRGYFPSPGWTGEYDWAGMVPFAQLPRDINP
                     ATGFLATANHRTVMANHVPHLTGSWYAPERHERIVQQLEADAGQTLSSTVALQGDVVD
                     LAALKLRDLLQAHDQRLRERIAHMPEGARESALKSLGMLTAFDGRMAIDAPGAALYGI
                     FLDSLTRQVFADELGAPEGQAWQSFLSANAITYSAQQDHMLGREDSPFWDDVKTAERR
                     EGKWDIVARTLAISWQQALFQLSADPANWRWGKLHTYHWESGSTKMKPFMPAVPGMVV
                     GLLGRYLDRGPFPAPGSTNTVNVAGYTIGDGYKVWNVPAMRLVVDFSLSEPLQVIIAG
                     GQSGNPASRHYDDGIPLYLSGDNRRMAFHDAGLVSEQFNRTLVLKPATVSEQPK"
BASE COUNT         1145 a         1890 c         1535 g         1003 t
ORIGIN      
        1 cggccgatgc ggtgcacgtg cacttccgga tcaaacgcca tttcgacatt gatcaccatg
       61 gcaagatcct tgatgtcgag gccgcgtgcc gccacatccg tcgccaccag caacgggcag
      121 ctgcggttgg aaaatttcac cagcaccaag tcacggtcgc gttgctccag atcaccatgc
      181 agcgcggcca cctggaagcc tttttcggac agcgccgtca ctacttcctc acagcgctgc
      241 ttggtattgc agaacagaac ggcagattcc ggcttgtgat gacgcaatac agcggccacc
      301 gcattcacgc gctcgcgatt gtccacttcg tggaaaatct gggcaatctt caccgcctcc
      361 ggcgcatcag ccaccgtgac ggacaccgga tcgcgctgga aacgcgtgct cagggcacgc
      421 acttcttttg agtacgttgc cgagaacaac agcgtctgcc gctttgaggg catctggtcc
      481 acaacttcga cgatgctgtc atggaagccc atgtccagca tgcggtcggc ctcatccagc
      541 accagcacct tcaacttgcc caaatcaagc gagcccttgg ccagatgctt cagtacacgg
      601 cccggcgtac cgacaatcac gtgggcacca tgctccagcg aatccagctg cggccccatg
      661 ggcacgccac cgcacagact cagcatcttg atgttgccag caaagcgcgc cagccgacgc
      721 atctccttaa gcacttggtc cgcaagctca cgcgtcgggc acagcaccag cgcttgcaca
      781 ctgaagtcac gcggattaag ggcactcagc acgccaatgc caaaggccgc cgtctttccg
      841 ctaccggttt tcgactgggc aatcacatcg cgccctgcga gcaatgaggg caggctctgc
      901 gcctggatgg gcgtcatggc gtggtagccc agctcgttca gggtatcgag cagggcttcc
      961 ggcagggact gggtggaaaa agcaggtgat tcaggcacag acgacacagg cgcaatccgg
     1021 gatgacaaac aggaggctca tggtaccact cccctcctcc ggcacgatgc ccggcgggcg
     1081 gattcccgcc ccaagccgta gcaacagtga ctcgtcagaa ctcgtcgccc tgttcctctg
     1141 ccgctttgcg tcgctcctcc tcgtcacggg catccatgat ttcgcggagc aggctgtcca
     1201 gatcggcagg cgtctcgtct tcctcgaatt cgccggtgag atggctttcc ggcgtcagtt
     1261 caccgctttc atacaaggcc cagatttcct tggcgtagcg ggtatcgagc agctccgggg
     1321 caaactggcc gaaataatcc gccaggttct gcacatcccg ctccagcatg gtgaaggcat
     1381 tgttgttgcc ggccgcatcc acggcctgcg gcagatcgat gatcaccggg cctttggggt
     1441 cgatcaggat gttgtattcg gacaggtcac catgcaccag acccgcacac agcatgcgca
     1501 ctacctcgtg caccagttgg tggtggcagg ccacggcttc ttccggcgta aaactgactt
     1561 cactcaggcg cggcgccggg cggccatgac catccgacac catgtccatg agcaacacgc
     1621 catcgaagca gccatgcggg gtaggcacgc gcacgccagc atcagcgaga cgatacaacg
     1681 cactcacctc ggcattctgc caggcctctt ccagttcctg cttgccaaag cgcgagccct
     1741 tctccattgc acgggcatca cgcgtattgc gcactttgcg gccttcgcga tacgtcgacg
     1801 cctgcttgaa gctgcggtgg tgagcatcct tgtacacctt ggcacaccgc agcacaccgc
     1861 cgcagcgcac caggtacacc gttgcttcct tgccgctttt gagctgtgcc accacctcgt
     1921 cgatcaggcc gtcttccaac agtggctcaa gtcgtttagg ggttttcatc cgggatgcat
     1981 cacgccacag tgttcacgcc gacacgggac gccaccatct tccagttgtc gtcgtcatgc
     2041 agcgacgtcc agctgtggcg gaacttgccc ttcatggcct cgaagcgacg ctgctccggg
     2101 aactggccac gcttttccag cgcctccagc acttccagcg cgccggcgcg gtggttgcaa
     2161 accagcaccc tgtcgcagcc ggcatccagc gccgcattgg cacgatcgct gaacgaaccg
     2221 gtggccacgg cgccttccat gctcaggtca tccgagaaaa tcacgccatc aaaacccagg
     2281 cgttcacgca ggatgtcctg cagccagaag gacgaaaagc cggccgggtt cgggtccact
     2341 ttcgaataaa tgacgtgcgc aggcatgatg ccctgcagct cgcgcgacag cgccgcaaac
     2401 ggtaccagat catgcgcttc gatctcggcc cacggacgct cgtcaaccgg cagggcaaca
     2461 tgtgaatcgg cctcaaccga gccgtgtccc gggaaatgct tgcccgtcgc tgccatgccg
     2521 gcctcgtgca tgccacgaat gaactcacgc agcagcgtca cggccacttg cggatcggca
     2581 ttgaaagcac gatcaccgat cacctgcgaa ataccacagt ccacgtccag aatcggcgca
     2641 aagctgatgt ccacaccaac cgccagcact tcactcgcca tcagccagcc acactggtag
     2701 gcctgctggc gggcttcgcc cggattctct gcatacaggt caccaaatac cttcatcgga
     2761 ggcagacgca cgaagccgtc acggaaacgc tgcacacggc cgccttcctg gtccaccgca
     2821 atcaggattt ccgggcgcac ggcgcggatt tcctgcacca gcgttgtcac ctgctccacg
     2881 ctctggaaat tgcgggcaaa caggatcagg ccgcccacat gcgggtgggc cagcaatttc
     2941 ttttcatcgt cggtgagggt gagtccctca acatccagca tcaaggcgcc aatcgccatg
     3001 aaccactcct cacatcattt cggttgttcg gacactgttg ccggcttgag caccagcgtc
     3061 cggttgaact gctcactgac cagcccggca tcatgaaacg ccatgcggcg attgtcgccg
     3121 gagaggtaca gcggaatgcc gtcatcataa tgacggctgg ccgggttgcc actctggccc
     3181 ccggcaatga tcacctgcaa cggctcagac aggctgaaat ccactaccag gcgcatggct
     3241 ggcacattcc acaccttgta gccgtcacca atggtgtaac ccgccacgtt gacggtattg
     3301 gtggaacccg gcgccgggaa cggcccacga tccagatacc gccccagcaa cccgaccacc
     3361 atgcccggta ctgccggcat gaatggcttc attttcgtgg aaccggattc ccagtgatag
     3421 gtatggagct tgccccagcg ccagttcgcc ggatctgcac tcagctggaa caaggcctgc
     3481 tgccaactga tggccagtgt tcgcgccacg atatcccatt tgccctcgcg ccgctcggcc
     3541 gttttcacat catcccagaa cggactgtcc tcacgcccca gcatgtggtc ctgctgtgcc
     3601 gaatacgtga tggcattcgc actcaggaaa gactgccagg cctgcccctc cggtgcaccc
     3661 agctcatcgg caaacacctg ccgggtcagg ctgtccagga agatgccgta cagggccgcc
     3721 cccggtgcat caatggccat acgcccgtcg aacgctgtca gcatcccgag cgacttcaac
     3781 gcagactccc gcgccccctc gggcatatgc gcaatacgct cacgcagacg ctggtcatgg
     3841 gcttgcagca agtccctgag cttgagtgcc gccagatcca ccacatcacc ctgcagtgcc
     3901 accgtcgatg acagtgtctg tccggcatcc gcctccagct gctgaacgat gcgctcatga
     3961 cgctccgggg cataccagga gccggtcagg tgcggcacat gattcgccat caccgtgcgg
     4021 tgattggccg tggccagaaa gcccgtggcc ggattgatgt cgcggggcaa ttgcgcgaag
     4081 ggcaccatcc ccgcccaatc gtattcgccc gtccagccgg gcgacgggaa atagccgcgc
     4141 cccttccggc gctgcggata caggccagtc acttgccagc cgatattggc ggcatcggca
     4201 aacagcaaat taaggtgaat ggcaccgatg ccctggatag cctcgccgac cgccgcaaaa
     4261 tcagcggcct gccccagccg gaagaaacgc tccatgctga ggtcagccac ccccgccgtt
     4321 gtctggaccg ccagaccata gccgccgctg acccgctgcg tgggcagcat gacctcgttc
     4381 acacggcggc ctcgtaatgc gtcgttcagc aaaggcccat gcaccgtctc ctgcacagtc
     4441 accacatgat ccggctcgcc cttgatacgg atgacttcac ggcgttcggt cacgggctgc
     4501 cattccccgc ggtacaggta gaaggtcttg ccgttttcct gcttcagctt ttcaacaaag
     4561 acatcctgcg aatcagccat gaccatggtc atgccccaag ccacatgacc gttgtagccc
     4621 gcgaccacac cgggcaggcc ggcaatcgca atgccaccgg cgcgataacc cggcgcactg
     4681 acctgcacca gcatccacac cggcggatgc tccagcatca ggtgcgtgtc attggccaga
     4741 atactggcct tgcccttggt acgtgcagca gccaccgccc aattattgga cgccgcctgc
     4801 cgcacgggca caaaaagact ggcgagctgc tggctgacac gggactgggc aacaacattt
     4861 gtctgcaagt ccttgaaggg gaagtcgcca agcttggcgg cctcgtccaa cgccaccggc
     4921 tcatccggat aagacggaat cagccacgca gccttctccg gccccaccac ccgcgccagg
     4981 tccaggaacg caacttcctc cggcaggttc agcgacaacc cgaggttgag catcatgaag
     5041 acatccatcg aatggagggg tgtccaggct tccggcgcat aagcactaag cttgaagtcc
     5101 agcggcaagc gatcctgatg cgatgccatc caggcattca caccatcggc aaagcgctgc
     5161 aacttgtgcc ggagcgccgg cgacgccagc ttgaggtgct gctcggcatg acgatgcaat
     5221 cccagcgtac gcatgtagat gtcgaggtca cgggttgtag cgcccgccat ctcggacagt
     5281 cgcccctgtg ccaccagcga ataggacacc atttgcgcca gccggtcgga cgccatgaca
     5341 tagcccaggc caaaggccag atcgtcttca ctggcagcct ctaccaacgg caccccgaac
     5401 gcgtcccgac gaatcgtgac cggtgccgac acgcctttga gctggaccac gccctcggcc
     5461 ttgtcgacgg tattgcgcag catcaggtcc atggcatcgc agcccgagag ggctagcacc
     5521 atcaggggaa tggcaagaag ggaaaaacgt cgacgcatga ccaagtccgg aag
//
LOCUS       DASUEG010001035         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_224454, whole genome shotgun sequence.
ACCESSION   DASUEG010001035 DASUEG010000000
VERSION     DASUEG010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224454"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..>2602
                     /locus_tag="VFX11_19445"
     CDS             181..>2602
                     /locus_tag="VFX11_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX4940858.1"
                     /translation="MNISVPRYLIGIDLGTTHTVVAYADLQNGADNAEPTLFEIEQLI
                     APGEVARRPLLPSFRYHPAPGELPEEQLVLPWRAQPLPGDDVLHVTGELARELGSKVE
                     GRQIASAKSWLSHDKVDRSAPILPWAAAEGVAKASPVVASASYLNHVRCAWNHDHPEH
                     PLEQQAVVITVPASFDEAARSLTLQAADMAGYPAVTLLEEPQAVCYDWFHRHRDTAAQ
                     QLQQSRLLFVVDVGGGTTDLSLIRVKTTNGQLQLDRIAVGDHLMLGGDNVDLALAHQA
                     EQRISGEGKKLSAASLSQLIQQSRKAKELLLGEQPPASAKVTLLGSGARLIGGARSAE
                     FSREEVQALALDGFFPPINFGEQPARRSSAMVEFGLPYAPDPAVSRYLSQFLSTHQNS
                     CREALGLPATATAVPDTVLFNGGVFKSSALKNRALDVLGQWRGETVTELHNQHPDLAV
                     AFGAVAFALAQRTSTLKIGGGSPRSYFLQVQTRSGDNKGVCLLPKGTEEGAEIALHEH
                     RFALRLNQPVQFHLAACSGDDQFSPGMLVDLNDDRFLPLPPLVAALDDSDAQNEIPVT
                     LVTRLTEVGTLDIHCQSEADPSRRWKVEFQLRRDLQAKHQRSALPPKFKDAIAALDEV
                     FGPGDKQADPNAVKRLRPTIEKLLGERDQWDTALARALFDALWERSKRRRRSAQHERV
                     WFNLAGYCLRPGFGYPADDWRISQVWALYAQGLQFNQESQSWAEWWTFWRRAAGGLDQ
                     TAQQRILKDIAKFLNPATARNLKIQQEIKNKSYEDMVRLAAALENLDLPTKTELGDWL
                     LKRLEKSSE"
BASE COUNT          523 a          823 c          792 g          464 t
ORIGIN      
        1 gacagaacag ttttcgcgct taggtccccg agctgcacgg tcagacctgc cattccagac
       61 acgccacaag tacatccctg taggctcgac agcggccatc catggccgct gacgtccctg
      121 aacaccaacc ttcctccact ccggcttcag ccactacttt cccgcgtgag tcgtttttaa
      181 atgaatatca gcgtcccccg ctacctcatc ggcattgacc tcggcactac ccacacggtg
      241 gtggcatatg ccgatctgca aaacggtgcg gacaatgccg agcccaccct gttcgaaatt
      301 gaacagctga ttgcaccggg cgaagtggcg cggcgaccgc tgctgccgtc gttccgctat
      361 caccctgcgc ccggagaatt acccgaagaa cagctggttt tgccatggcg cgcgcaaccg
      421 ttgccgggtg acgacgtgct gcatgtcacc ggcgaactgg cgcgggaact gggcagcaag
      481 gtggaaggcc gccagattgc gagtgcgaaa agctggctgt cccacgacaa ggtggatcgc
      541 tccgcgccga tcctgccctg ggcggcggcc gagggtgtcg cgaaggcatc gccggtggtg
      601 gccagtgcgt cctacctgaa ccatgtgcgt tgtgcctgga atcacgatca tcccgaacat
      661 ccgctggagc agcaggcagt tgtcatcacc gtgccggcat cgttcgatga ggcggcgcgc
      721 tcgctgacat tgcaggccgc cgacatggcc ggttaccccg ccgtgactct gctggaagag
      781 cctcaggccg tttgctacga ctggtttcac cgccaccgcg atactgccgc acaacaattg
      841 cagcaaagcc gcctgttgtt tgtggtggac gtgggcggcg gcaccaccga cctcagcctg
      901 atccgcgtga aaaccaccaa cggccagttg cagctggacc gcatcgcggt gggcgaccac
      961 ctcatgctgg gcggcgacaa tgtggatctg gcgctggccc atcaggccga acagcgcatt
     1021 tccggcgaag gcaaaaaact gtccgccgcg agtctgtcgc aactgattca gcaaagccgc
     1081 aaggcgaagg aattgttgct gggggaacag ccacctgcat ccgcgaaagt cacgttgctg
     1141 ggcagtggtg cgcgtttgat tggcggcgcc cgcagcgcgg aattttcccg cgaggaagta
     1201 caggcgctgg cgctggacgg atttttcccg ccgatcaatt ttggcgagca accggccaga
     1261 cgcagcagcg cgatggtgga attcggcctg ccgtatgcgc ctgatccggc cgttagccgc
     1321 tatctgtcgc agtttctgtc cacgcaccag aacagctgcc gggaagcgct gggtttgccg
     1381 gcaaccgcaa ccgccgtccc cgacaccgtc ctgttcaacg ggggtgtctt caaaagttcg
     1441 gccctgaaaa atcgtgcgct ggacgtgctg ggccagtggc ggggtgaaac cgtcaccgaa
     1501 ttgcacaacc agcaccccga tctcgcggtg gcgttcggtg ctgtggcatt tgcgctggcg
     1561 caacgcacct ccaccctgaa aatcggcggc ggctccccgc gcagttattt cctgcaggtg
     1621 caaaccagat cgggtgataa caagggcgtg tgcctgctgc ccaagggcac ggaagagggt
     1681 gccgaaatcg cactgcacga acaccgtttc gcgttgcgcc tgaatcagcc ggtgcagttc
     1741 cacctggcgg cctgttccgg tgacgatcag ttttcccccg gcatgctggt ggatctgaac
     1801 gacgaccgct ttctgccgct gccgccgctg gtggccgcac tggatgacag cgatgcgcaa
     1861 aatgaaattc ccgtcacgct ggttacgcgc ctgacagaag tcggtacgct ggatattcac
     1921 tgccagtcgg aagccgatcc gtcccggcgc tggaaagtgg aattccagtt gcgtcgtgat
     1981 ctgcaggcga agcatcagcg cagcgcactg ccacctaaat tcaaagacgc gatagccgcc
     2041 ctcgacgaag tgttcggccc cggcgacaaa caggccgacc ccaacgcggt gaaacgcttg
     2101 cggcccacga tcgaaaaact gctgggcgaa cgtgatcagt gggacacagc cctggcccgc
     2161 gcgctgttcg acgcgctgtg ggagcgcagc aagcggcgcc ggcgcagcgc acagcacgaa
     2221 cgggtctggt tcaatctggc gggttactgc ctgcgccccg gcttcggtta tccggcagac
     2281 gactggcgca tcagccaggt ctgggcgctg tacgcgcagg gcctgcagtt caatcaggaa
     2341 agccagtcct gggcggaatg gtggaccttc tggcgtcgtg ccgccggtgg cctcgaccag
     2401 accgcacagc agcgcatcct caaggacatc gccaaattcc ttaatccggc cactgcccgc
     2461 aacctgaaaa tccagcagga aatcaaaaac aaatcctacg aggatatggt gcggctggcg
     2521 gcggccctgg aaaatctcga cctgccgacc aaaactgaac tgggcgactg gctgttgaaa
     2581 cgcctcgaaa aaagcagcga aa
//
LOCUS       DASUEG010001036         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1628985, whole genome shotgun sequence.
ACCESSION   DASUEG010001036 DASUEG010000000
VERSION     DASUEG010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628985"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFX11_19450"
     CDS             <1..1156
                     /locus_tag="VFX11_19450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4940859.1"
                     /translation="DAGISRLLDIPVSELEGRSWFSLFHPFDRNRIDAALKQLESAGQ
                     GHAVLRTAKASGATLYLYVNLCRAEGTNPGGDYYCLVQNLGDRTGYDASQQHYERLFS
                     LSNDLLCVASTMGYFLQVNPAFTRVLGYSSEELQRTTFLEFIHPDDVDATLNEIRKLR
                     SGQDTLYFENRYRCKDGSWRWLAWSTPASNEEGILYAVAKDITERKEHEDHLLRMAKF
                     DFLSGLPNRSYLEEELKRAMSRAGRGQQILVGYFVDLNDFHQVNERHGHDVGDDLIRH
                     MAVRLRTLLRACDFIARVGSDEFFVLAEVEHSSNALHLADKIGMLLHNPFTLEDGMIK
                     VSASVGTTLYQAEVHRDPSSFITSAERAMQMQKQKYKQINTGNDSRVAPA"
     gene            complement(1175..2086)
                     /locus_tag="VFX11_19455"
     CDS             complement(1175..2086)
                     /locus_tag="VFX11_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX4940860.1"
                     /translation="MNELRQERDLVLVVDDSPDTLGMLNHALDQAGFTVLVALEGLQA
                     VSIARKMLPDIILLDAVMPNLDGFATCRILKREPDLADVPVIFMTGLTDTDDIVKGLE
                     AGGVDYVTKPVKPQELIARIRVHLNNARLTQSARTALDTAGQFLFATTRDGQFLWATP
                     QVHHLFEKSGADRFWLDQQLTPQLRAGFPAQALPGSNLQLQGLSLPLKVSYLGEPAPG
                     ERLLRLTDPERPSETEALRKHLAVTEREAEVLVWIAHGKTNREIGQILSMSPRTVNKH
                     LEQIFRKLGVENRTTAAAMALRHLGTD"
     gene            complement(2083..>2944)
                     /locus_tag="VFX11_19460"
     CDS             complement(2083..>2944)
                     /locus_tag="VFX11_19460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4940861.1"
                     /translation="IFRPFERVRKSGANPVPGTGLGLTITRLLTDIMGGDIAVQSEPG
                     VGSTFTVSLMLSRIDTPCEAPVVEKQIRGYLGARRLLMVVDDDPSHRGLISDLLTPLG
                     FGVVEAACGADCLALARQCHADAFLLDVSMPGMTGWQLAESLRGNGVIAPIIMISADA
                     REGQRDQHDADTPPRWHDDYVVKPVRLQMLLEKLGALLRLTWFYERERAESEPVPQPF
                     LPEQLPSAEVLQELRTLAEIGYINGLKQKIQQLRQEGSATPEFIAMLQNLTSQFRFDR
                     IIEIAQTVKP"
BASE COUNT          576 a          910 c          886 g          572 t
ORIGIN      
        1 agacgcaggc atctcgcgac tgctggatat tcctgtgtcg gaactggagg gacgctcctg
       61 gttcagcctg tttcatccct tcgaccgcaa tcgcattgac gccgccctga agcagctgga
      121 aagcgcgggc cagggtcatg ctgtcctgcg cacggccaag gccagcggcg ccacgctgta
      181 cctgtacgtg aacctgtgcc gcgccgaggg gaccaatccc ggcggcgatt actactgcct
      241 ggtgcagaat ctgggagacc gtaccggcta cgacgcgagc cagcagcatt acgagcgcct
      301 gttctcgttg tccaacgacc tgctctgcgt ggccagcacc atgggttact tcctgcaggt
      361 gaatccggcg ttcacacgcg tactgggcta ctcttccgaa gaactgcaac gtaccacctt
      421 cctggaattc atccatccgg acgacgtgga tgccaccctg aatgaaatcc gcaagctgcg
      481 cagcggccag gacacgctgt atttcgagaa ccgttaccgc tgcaaggacg gcagctggcg
      541 ctggctggcg tggagcacac cggcgtcgaa cgaggaaggc atcctgtacg cggtggccaa
      601 ggacatcacc gagcgcaagg agcatgagga tcatctgctg cgcatggcga aattcgattt
      661 cctgtcagga ctgcccaacc gcagctatct ggaggaggaa ttgaagcgcg ccatgtcgcg
      721 cgcaggccgc gggcagcaga tactggtggg ctacttcgtc gatctgaatg atttccacca
      781 ggtgaatgaa cgccacggac acgacgtggg cgatgacctg atccgtcaca tggcggtacg
      841 tctgcgcacc ctgttgcggg cctgcgattt cattgcacgc gtcggcagcg atgaattttt
      901 tgtgctggcg gaagtggagc attcatcgaa tgcgctgcac ctggccgaca agattgggat
      961 gttgctgcac aatccgttca cgctggagga cggcatgatc aaggtgagcg ccagcgtcgg
     1021 cactaccctg taccaggccg aagtgcatcg tgatccttcg agtttcatca ccagcgctga
     1081 gcgggcgatg cagatgcaga agcaaaaata caaacagatc aatacgggca acgattcccg
     1141 cgtcgcgccg gcctgatcac cccgacattc cgtttcaatc cgtacccaga tgccgtaacg
     1201 ccatggccgc cgccgtggtg cggttttcca cgcccagctt gcggaagatc tgctccagat
     1261 gtttgttcac ggtgcgcgga ctcatgctga ggatctggcc gatttcgcga ttggttttgc
     1321 catgggcgat ccacaccagc acctctgctt cccgctcggt gaccgccaga tgtttgcgca
     1381 gcgcttcggt ttccgacggc cgttccggat cggtcagacg cagcagacgt tcccccggcg
     1441 ccggctcccc cagataactg accttgagcg gcagtgaaag cccctgcaat tgcagattgc
     1501 tgccgggcag tgcctgcgcc ggaaaacccg cccgcaattg cggcgtgagt tgctggtcga
     1561 gccagaagcg gtcggcaccg gatttttcga acaggtgatg cacctgcggc gtagcccaca
     1621 ggaactggcc gtcgcgggtg gtggcaaaca ggaactggcc ggcggtgtcc agtgccgtgc
     1681 gcgcgctttg cgtcaggcgc gcgttgttca ggtgcacccg gatgcgggcg atcagctcct
     1741 gcggtttcac cggcttggtg acgtaatcaa caccaccggc ttccagccct ttcacaatgt
     1801 cgtcggtgtc ggtgaggccg gtcatgaaaa tcaccggcac gtccgccaga tccggttcgc
     1861 gtttcaaaat gcggcaggtg gcgaagccat ccaggttcgg catcactgcg tccagcagaa
     1921 tgatatccgg cagcattttg cgggcaatgc tgactgcctg caatccttcc agcgccacca
     1981 gcacggtgaa accggcctga tccagcgcgt gattgagcat gccaagggtg tcgggcgagt
     2041 catccaccac caacaccaga tcccgttcct gccgcagctc attcatggtt tcaccgtttg
     2101 tgcaatttca atgatgcgat cgaaccggaa ctggctggtg agattctgca gcatggcgat
     2161 gaattccggt gtggcgctgc cctcctggcg cagttgctgg attttctgct tgagtccatt
     2221 gatgtagccg atttccgcca gcgtgcgcag ttcctgcagc acctccgcag acggcaactg
     2281 ttccggcaaa aagggctgtg gtaccggctc ggattccgca cgctcacgct cgtaaaacca
     2341 cgtcagccgc agcagcgcgc cgagcttttc cagcagcatc tgcaggcgca ccggtttcac
     2401 tacatagtca tcatgccagc gcggcggtgt gtcggcatcg tgctggtcac gctggccttc
     2461 gcgggcgtcg gcggaaatca tgattatggg cgcaatcacg ccattgccgc gcagggactc
     2521 ggccagttgc cagccggtca tgccgggcat ggacacatcc agcagaaatg catcggcgtg
     2581 acactgccgc gccagcgcca gacaatcagc accgcacgcc gcttccacca caccaaaccc
     2641 cagcggcgtg agcagatcgc tgatcaggcc acggtgggac ggatcatcat ccaccaccat
     2701 cagtaatctg cgcgcaccga ggtagccgcg aatctgtttt tccaccaccg gcgcttcgca
     2761 gggcgtgtcg atgcgcgaca gcatcaggct gacggtaaag gtactgccaa cgccgggctc
     2821 gctctgcacg gcaatgtcgc cacccatgat gtcagtgagc agacgcgtga tggtgagtcc
     2881 cagcccggta ccgggcacgg gattggcgcc ggatttacgc acccgttcga agggacggaa
     2941 gatg
//
LOCUS       DASUEG010001037         7373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1761482, whole genome shotgun sequence.
ACCESSION   DASUEG010001037 DASUEG010000000
VERSION     DASUEG010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761482"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VFX11_19465"
     CDS             complement(<1..1465)
                     /locus_tag="VFX11_19465"
                     /inference="COORDINATES: protein motif:HMM:NF024205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940862.1"
                     /translation="MNSLFRPAFWALIWSVLLVLVPPVLAAGEPEKAAESGSGPNLES
                     QLQQLQEEQKLLARQNAEINSQMQQAPGLVEQMRREISRLERQPASPKIPNSYEALES
                     AIELLDAQAKSIQLSLSDVMDRISEQQALPTQASEQVTAAQRQIQDLEAQLRAALQAD
                     QTLPANNLQVQVLRQQLANAKLRRDIAGNGLEGYQKLLEMHTVNRDLLLLRLSALKTA
                     LELLRTARDSQREKNAETQQEDNDLSQSEWEQKPKPIVTQLEENQRLATLLNTVSDSL
                     VHTNDAIADGKQELQDLRYRFDIAEQQLQLTDFHQYVDDYLLRQRQSLQVRIWEQQDS
                     SDLTEQISQARLDQFKFDDQLHQVRTSAARQRKIELVLKKIPDLDEAGRAQYATEVQN
                     ILTQREEVLSRLLEVNAKLVVSLTNLQLLQQDQLTQRQQFFELLNEKLLWRRSGTPLS
                     LGWLQKLPGSLHWFVTHPGWLELVQVWYRAIVQPVFPL"
     gene            1597..3267
                     /gene="ettA"
                     /locus_tag="VFX11_19470"
     CDS             1597..3267
                     /gene="ettA"
                     /locus_tag="VFX11_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX4940863.1"
                     /translation="MAQYIFTMHRVSKVVPPKREILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDKEFDGEARPATGIKIGYLPQEPQLDPAKDVRGNVEDGVREIKDVLT
                     RFDEISMKFGEEMSDDDMNKLLEEQAELQNKIDACNGWDIDRTLEIAADALRLPPWDA
                     DVTKLSGGEKRRVALCRLLLSAPDMLLLDEPTNHLDAESVAWLERFLHDYKGTVVAIT
                     HDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEARLEQEAKQEASRQRSIKSELE
                     WVRANPKGRQAKSKARIARFEELTSVEAQKRNETQELYIPVAERLGDDVIEFRDVTKS
                     FGDRVLYDHLNFKIPKGAIVGIIGPNGAGKTTLFRLINGEQKPDSGEVHIGSTVQLAY
                     VDQSRDSLNNNRTVWEEVSDGLDFIKIGSYEMASRSYVGRFNFKGADQQKRVGELSGG
                     ERNRLHLAKLLRTGGNVLMLDEPTNDLDIETLRALEEALLTYAGVAIVISHDRWFLDR
                     IATHILDFEGEEGVEFFEGNFTDYEAYKKKKYGEEATQPKRLKYRKLV"
     gene            complement(3464..3682)
                     /locus_tag="VFX11_19475"
     CDS             complement(3464..3682)
                     /locus_tag="VFX11_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940864.1"
                     /translation="MRTTSTTNETQFVEEVDEVLMDDIDNEIEKRLEGKSKRSGDYLR
                     KIEALMEERRLQRELGYLDDDSEGGDEE"
     gene            4026..4394
                     /locus_tag="VFX11_19480"
     CDS             4026..4394
                     /locus_tag="VFX11_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4940865.1"
                     /translation="MLEKRNFTRIAFDARARLLKGESDWATHLLDISLKGALVELPAG
                     SALKTGDAVSLHLLLSDEETEINMEGHVVHQEGNHVGLVCDHIDVDSVSHLRRIVELN
                     TGSEALLEREFNALTRFGKT"
     gene            complement(4447..5013)
                     /locus_tag="VFX11_19485"
     CDS             complement(4447..5013)
                     /locus_tag="VFX11_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX4940866.1"
                     /translation="MEAFLSSTFAVAIAEIGDKTQLLSLFLATRFTQRYAIIAGILVA
                     TLLNHAASAWLGVFVMQWVPAQWGHWLIAGSFVLIALWLLIPDKDDGAPSALDRYGAF
                     IATTVLFFLAEIGDKTQIATVVLAAHYHSTFWVVAGSTLGMLAANVPVVLAGEWLMQR
                     MPLRYARWGACALFLVLAVVTLVNGGKG"
     gene            complement(5213..6580)
                     /gene="radA"
                     /locus_tag="VFX11_19490"
     CDS             complement(5213..6580)
                     /gene="radA"
                     /locus_tag="VFX11_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX4940867.1"
                     /translation="MAKAKTQYVCNDCGSVFAKWAGQCTDCGAWNTLTEFNPDHGMGP
                     RTGKGAKTGWAGEARKVTTMAEVALVDVPRVASGSAELDRVLGGGLVEGSVVLIGGDP
                     GIGKSTILLQTMTHLASRLPALYITGEESLQQVALRARRLDLPMDALRIMSETSIENI
                     LATAAKEQPKVMVVDSIQTVYSEALTSAPGGVSQVRECAAALVRYAKQSGTALFLAGH
                     VTKEGALAGPRVLEHMVDCVLYFEGEQDNRFRMIRAVKNRFGAVNELGVFAMTDKGLR
                     EVSNPSAIFLSRYDHPTPGSIVMVTREGSRPLLVEVQALVDDSQLGNPRRVAVGLDQN
                     RLAMLLAVLHRHGGVATMGMDVFLNVVGGVKVLETGSDLAVLTAVVSSLRNRALDNRL
                     IAFGEVGLSGEIRPVPNGQERLKEAAKHGFARAIVPKGNVPKAPIPGMEVVGVTRLEE
                     ALDAL"
     gene            complement(6608..6994)
                     /locus_tag="VFX11_19495"
     CDS             complement(6608..6994)
                     /locus_tag="VFX11_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940868.1"
                     /translation="MPFWVTLYIAFMVISLPVGVYMLRQLENDWLHPVGGLVSTLLSI
                     AFIFAYWMPDTVPFNTPSTILMYVFVLFWDLYTLKRVRTKLPEYLNMSEDSELQPDSS
                     TWLLGIVLMAPAYYFGALVCLSIVTS"
     gene            complement(6996..>7373)
                     /locus_tag="VFX11_19500"
     CDS             complement(6996..>7373)
                     /locus_tag="VFX11_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4940869.1"
                     /translation="QLESRIISTSVVPAGETVGYSATWRAAKDTPIGVVAIGYGDGYP
                     RHVSPEAFVLIKGQRCPLVGRVSMDMLTVDISNVPDATLETPVILWGRGLPVEWVARW
                     AGTINYELLCQVTDRVERIYAAG"
BASE COUNT         1513 a         2360 c         2129 g         1371 t
ORIGIN      
        1 gcagcggaaa caccggctgg acaatggcgc gataccagac ctgcaccagc tccagccagc
       61 cgggatgggt gacaaaccag tgcaggctgc ccggcagctt ctgcaaccat ccaagactca
      121 gaggcgtgcc gctgcgccgc cacaacagtt tttcattgag cagctcgaag aactgctggc
      181 gttgtgtcag ctggtcctgc tgcagcaatt gcaggttggt caggctcacc accagcttgg
      241 cgttcacttc cagcaggcgt gacagcactt cctcgcgctg ggtcagtatg ttttgcactt
      301 ccgtcgcata ttgggcgcgg ccggcttcat ccagatcggg aatctttttc agcaccaact
      361 cgatcttgcg ctggcgggcg gcgctggtgc gcacctggtg cagctggtca tcgaacttga
      421 actggtccag cctcgcctgg gaaatttgct ccgtcaggtc gctggaatcc tgctgttccc
      481 agatgcgtac ctgcaggctc tgacgctggc gcagcaggta atcatccaca tattggtgga
      541 aatccgtgag ctgcaactgc tgctcggcga tatcgaaacg gtagcgcaga tcctgcaatt
      601 cctgcttgcc gtcggcgatg gcatcattgg tgtgcaccag gctgtcggag actgtattca
      661 gcagtgtcgc caggcgctgg ttttcctcca gctgggtcac gatgggcttg ggcttttgct
      721 cccattccga ctgcgacagg tcgttgtctt cctgctgtgt ctcggcattc ttctcgcgct
      781 gggaatcccg cgccgtccgc aacagttcca gcgcagtctt cagcgcactc agccgcagca
      841 gcaacaagtc acggttgacg gtatgcatct ccaggagctt ctggtagccc tccagaccat
      901 tgccggcaat gtcccggcgc agcttggcgt tggccagctg ctggcgcagt acctggacct
      961 gcaggttatt ggcaggcagg gtctggtccg cctgcagggc agcccgcaac tgggcctcca
     1021 gatcctgaat ctggcgctgg gcggcggtga cctgttcgct ggcctgggtg ggcagcgcct
     1081 gttgctcgct gatccggtcc atcacgtcag acaggctcag ctggattgac ttggcctgcg
     1141 catccagcag ctcgatggcc gattccagcg cctcgtagga attgggaatc ttcggactgg
     1201 caggctgtct ctccagccgg ctgatctcgc gccgcatctg ctcgaccagg cccggcgcct
     1261 gctgcatctg gctgttgatc tcggcgttct ggcgcgccag cagcttctgc tcttcctgca
     1321 gctgctgcag ctgcgattcc aggttggggc ccgatccgga ttcagcggcc ttctctggct
     1381 cgccggcagc aagcaccggt ggaaccagca ccagcagcac agaccagatc aacgcccaaa
     1441 aagcaggcct gaaaagcgag ttcacacagg ctcctttccc ggctgaaccg gggtatttag
     1501 atcaaggatg gaagtttgca tcgccctggc aattggagta gcgtagcgcc gaatcccaac
     1561 aacccttccc ggcaattaca cagtattcaa taagctatgg cgcaatatat tttcaccatg
     1621 catcgggtca gcaaggtcgt gcccccgaag cgcgaaattc tcaaggatat ctccctgtct
     1681 ttcttccccg gcgccaagat cggcgtactc ggcctgaacg gctcgggcaa gtccacgctg
     1741 ctgcgcatta tggcaggtgt cgacaaagag ttcgacgggg aagcccgccc tgccaccggc
     1801 atcaagatcg gttacctgcc gcaggagccc cagctggatc ccgccaagga cgtgcgcggc
     1861 aatgtggaag acggcgtgcg tgaaatcaag gatgtgctga cccgcttcga cgaaatcagc
     1921 atgaagttcg gcgaggaaat gtcggacgat gacatgaaca agctgctgga agagcaggcc
     1981 gaactgcaga acaagatcga cgcctgcaac ggctgggaca tcgaccgcac cctggaaatc
     2041 gccgccgacg ccctgcgcct gccgccctgg gatgccgacg tgaccaagct gtccggtggt
     2101 gaaaagcgcc gggtggccct gtgccgcctg ctgctgtccg cccccgacat gctgctgctg
     2161 gacgaaccga ccaaccacct ggacgccgag tccgtcgcct ggctggaacg cttcctccac
     2221 gactataaag gcaccgtggt ggccatcacc cacgaccgtt acttcctcga caacgccgcc
     2281 gagtggattc tggaactgga ccgcggccac ggcatcccct ggaaaggcaa ctattcgtcc
     2341 tggctggaac agaaggaagc ccgcctggaa caggaagcca agcaggaagc ctcacgccag
     2401 cgcagcatca aatccgaact ggaatgggta cgcgccaacc ccaagggccg tcaggccaag
     2461 agcaaggcgc gtatcgcccg cttcgaggaa cttaccagcg tcgaagccca gaagcgcaac
     2521 gaaacccagg aactctacat ccccgtggcg gagcggctgg gggatgacgt catcgaattc
     2581 cgcgacgtca ccaaatcctt cggcgaccgc gtgctgtacg accacctgaa tttcaagatc
     2641 cccaaaggcg ccatcgtcgg catcatcggc cccaacggtg ccggtaaaac caccctgttc
     2701 cgcctgatca atggcgaaca gaagcccgac agcggcgaag tgcacattgg ctccaccgtg
     2761 cagctggcct acgtcgacca gtcccgtgac agcctgaaca acaaccgcac cgtgtgggaa
     2821 gaagtgtccg acggtctcga tttcatcaag atcggctcct acgaaatggc atcacgctcg
     2881 tacgtgggcc gtttcaactt caagggcgct gaccagcaga aacgggtggg cgaactgtcc
     2941 ggcggcgagc gcaaccgtct gcatctggcc aagctgctgc gcactggcgg caacgtgctg
     3001 atgctggacg aaccgaccaa cgacctcgat atcgaaaccc tgcgggccct ggaagaagcg
     3061 ctgctgacct acgccggcgt ggccatcgta atctctcacg accgctggtt cctggaccgg
     3121 attgcaaccc acatcctgga tttcgaaggc gaggaaggcg tggaattctt cgaaggcaac
     3181 ttcaccgatt acgaagccta caagaagaaa aaatacggcg aggaagccac ccagccaaaa
     3241 cggctcaaat accggaagct ggtctgaccg caaacgaaac gatcgaagtg aaaacggaaa
     3301 ggggcgcaaa tgcgcccctt ttgctgcatg tcatattggc actgaacacg ggtgcatcaa
     3361 cccggaacac tcacggcaca ggaccggcac gctgcacagc accgaaaatc cgccgccccg
     3421 gcccctcacg gtttccggca gtggccagtt gaaggcagtg cggttactcc tcgtcgccgc
     3481 cttcgctgtc gtcatcaaga taccccagct cccgctgcag gcggcgctct tccatcagcg
     3541 cctcgatttt tctcaggtag tcgcctgacc ttttgctttt tccttccagg cgtttttcga
     3601 tttcattgtc gatatcgtcc atcaggactt cgtcgacttc ttcaacgaat tgagtttcat
     3661 tggtggtgga tgtcgttctc ataaccccat tcctgcacaa tgtttcagag ttgtgaaatc
     3721 aggcactctc gttcccaacc agcgcatctt ccatgaaagc ctggcggctt tcatctttta
     3781 attattaacg cagcatcaaa aaaatttgta gttcctttta acttttctta gagttttttc
     3841 ccatgagcgg tcaccggttt atgcgttgca aagcaccgaa ccgccacgac cggcttttgg
     3901 taacacggat ttgcgaaccg taacaaaaac gccacagggc caacttgaat ttggcggaaa
     3961 ttctgcgttt tattaaagat tggactatgc tggatgcaat ggactgatca gggcgtaccg
     4021 ccatcatgct agaaaaacgc aatttcaccc gcattgcctt cgacgcccgc gcgcgcctgc
     4081 tgaagggcga atccgactgg gctacacacc tgctggatat ttccctgaag ggggcgctgg
     4141 tggaactgcc agccggttcc gcactgaaaa cgggtgacgc ggtgtcgctg cacctgttgc
     4201 tcagtgacga ggaaaccgaa atcaacatgg aggggcatgt ggtgcaccag gaaggcaatc
     4261 atgtaggact ggtgtgcgac catatcgatg tggacagcgt gtcgcacctg cgccgcatcg
     4321 tggaactcaa caccggcagc gaggcgttgc tggaacggga gttcaacgca ctgacccgat
     4381 tcggcaaaac ctgatccagc cccccagccc gggccagagc gcaccacgcc ccggcccctc
     4441 cgcaacctat cccttgcccc cgttcaccaa cgttaccacc gccagcacca gaaacagggc
     4501 gcacgcaccc cagcgcgcat agcgcagtgg catccgctgc atcagccact cgccggccag
     4561 caccaccggc acgttcgccg ccagcatgcc cagcgtactg cccgccacca cccagaacgt
     4621 tgaatggtaa tgcgccgcca gcaccacggt ggcgatctgc gtcttgtcac cgatttccgc
     4681 cagaaagaac agcacggtgg tggcaatgaa tgcgccgtac cggtccagcg cgctgggcgc
     4741 gccatcgtcc ttgtcgggaa tcagcagcca gagcgcgatc agcacgaaac tgccggctat
     4801 cagccagtgg ccccactggg ccggaaccca ctgcatgacg aacacaccca gccaggcaga
     4861 agcggcgtga ttgagcagtg tggcaaccag aatgccggca atgatggcgt agcgttgggt
     4921 gaagcgggtg gccagaaaca gcgacagcag ctgggtcttg tcaccgattt cggcaatggc
     4981 gacagcgaag gtggaactga ggaaggcttc catgaacagg tctcggcggg aggttggaca
     5041 caaggcaacg gcgactgtcc cgcccgggca gtggctgttg cctcaagtct caccagtcca
     5101 caggttgctg tggatgccac gaccataagc cttcggctca attatgttga tcgtgacgct
     5161 ggcacggggc cagccggtta ctcccctaag gtgcgagaat tctaacagga aatcacagcg
     5221 cgtccagcgc ctcttccagc cgcgttaccc ccaccacctc catgccggga atcggcgctt
     5281 tcggcacgtt gcccttcggc acgatggcgc gggcaaaccc atgtttggcc gcttccttca
     5341 gccgctcctg gccattgggc accggacgga tctcgccgga caaccccact tcgccgaacg
     5401 caatcaggcg attgtccagc gcccggttgc gcagactgga caccacggcg gtgagcaccg
     5461 ccagatcgga gccggtttcc agcactttca ccccgcccac cacgttgagg aacacatcca
     5521 tgcccatggt ggcgacgccg ccgtgccggt gcagcaccgc cagcaacatg gcgaggcggt
     5581 tctggtccag ccccaccgcg acgcggcgcg gattgcccag ctggctgtcg tccaccagcg
     5641 cctgcacctc caccagcagc gggcggctgc cttcccgcgt caccatgaca atactgccgg
     5701 gtgtgggatg gtcgtaacgg gacaggaaaa tcgccgaggg attggacact tcccgcaggc
     5761 ccttgtccgt catggcgaaa acgcccagct cattgactgc gccaaagcgg ttcttcacgg
     5821 cgcggatcat gcggaagcgg ttgtcctgct cgccttcgaa atagagcacg cagtcgacca
     5881 tgtgttccag cacgcgaggg ccggccagag caccttcctt ggtgacgtgg cccgccagga
     5941 acagcgccgt gccactctgc ttggcgtagc gcaccagtgc ggcggcacac tcccgcacct
     6001 gcgacacacc gccgggggct gaggtgagcg cttcgctgta aacagtctgg attgaatcca
     6061 ccaccatcac cttgggctgc tccttcgcgg cggtggcgag gatgttctcg atgctggttt
     6121 ccgacatgat gcgcagcgca tccatgggca ggtccagccg gcgggcccgc agggccacct
     6181 gctgcaggga ttcctcaccg gtgatgtaga gagcgggcag gcgcgaggcc agatgggtca
     6241 tggtctgcag caggatggtg gacttgccga tgccaggatc gccgccgatc agcaccaccg
     6301 agccctccac caggccgccg cccagcaccc ggtccagttc cgccgagccg gatgccaccc
     6361 gcggcacgtc caccagcgcc acttccgcca tggtggtgac cttgcgcgcc tcccccgccc
     6421 agccggtttt tgcgcccttg ccagtgcgag ggcccatgcc gtggtcggga ttgaactcgg
     6481 tcagcgtatt ccaggcgccg cagtcggtgc actgtcccgc ccacttggcg aacacactgc
     6541 cgcagtcatt gcagacatac tgggtcttgg ccttggccat gtttggcatt ccttttcagg
     6601 cgatgattca ggaggtgacg atgctcagac acaccagcgc accgaaataa taggcgggcg
     6661 ccatcagcac aatgcccagc agccaggtac tggaatcggg ctgcaactcc gaatcctcag
     6721 acatgttgag gtattccggc agtttggtgc gcacgcgctt gagggtatac agatcccaga
     6781 acaggacaaa cacgtacatg agaatcgtgg acggcgtgtt gaagggcacg gtgtccggca
     6841 tccagtaggc gaatatgaat gcgatgctga ggagagtgga gaccaggccg cctaccggat
     6901 gcaaccagtc gttttccagc tgccgcagca tgtagacccc taccggcagg gagatcacca
     6961 tgaacgcgat gtagagcgtc acccagaacg gcatgtcagc ccgccgcgta gatgcgctcg
     7021 acccggtcgg tgacctggca cagcagctcg taattgatgg tgccggccca gcgcgccacc
     7081 cattccaccg gcagcccccg gccccacaga atcaccggcg tttccagcgt ggcatcgggc
     7141 acattgctga tatccaccgt cagcatgtcc atggacaccc ggcccaccag cgggcaacgc
     7201 tggcccttga tcagcacgaa ggcttccggc gacacgtgac gcggataacc atcgccatat
     7261 ccaatcgcca ccaccccgat gggcgtatct ttggccgcac gccaggtggc gctgtagccg
     7321 acagtttccc cggccggcac caccgacgtc gagatgatgc gggattccag ttg
//
LOCUS       DASUEG010001038         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1806093, whole genome shotgun sequence.
ACCESSION   DASUEG010001038 DASUEG010000000
VERSION     DASUEG010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806093"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1542)
                     /locus_tag="VFX11_19505"
     CDS             complement(289..1542)
                     /locus_tag="VFX11_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4940870.1"
                     /translation="MAISKITLAEWRSFWFLPFAAALGYSTSVIHVYSLGPFIEPLQQ
                     AFGWSRAEASLGISISSLISAIFCIPIGMLVDRVGPRRVGLVGVLLMLSAFALLGTTT
                     GGIANWILLWVVVAFSTLWVQATVWTSAVASRFESSRGIAFAITLSGASLSATVFPLL
                     ATWLIGAYGWRTAFSAMGGIWAAMVFPVLFLFFRGAQDVLRKQGAVVPGAAKILAGLT
                     LSEGLRSPKLYKLLLASGLFSFTAIGALVHFVPILTDSGTTPLAAAGVASLIGIFSII
                     GRLGTGLLLDKFPGHLVGAAAFMLPIVSAVLLLFAGSDPVSQSIAAAALGLTVGSEVD
                     VIAFLAAKHFGLKNFGALYGALVMALSMGTAFGPLTAGYVFDFSGSYAPFLMLAIVLM
                     AVSAVALLSLGPAPETHEADTVPAH"
     gene            complement(1553..>2153)
                     /locus_tag="VFX11_19510"
     CDS             complement(1553..>2153)
                     /locus_tag="VFX11_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940871.1"
                     /translation="YLIEVNGTPQLKLRADVLPADLSLPMEELVATGFIMTAMPVVNA
                     IPSVVAARPGIVTYADLPPVTSIFRPKAIVSAPVATVGAAEPAMATSRSTVKTENPAA
                     PIEGDWKITIKAPTGPLQTLLNLQFRDGCLTGTQSGDGTVAPIVDAKLEGNNISWINH
                     ATKPIKLKCEFKAVLDGRQMAGKMKAGFMGSYTFTGVKV"
BASE COUNT          537 a          634 c          590 g          392 t
ORIGIN      
        1 gcgctgttgt acagatagaa attgagttgt gcaccgtccg tttgaacgct gggagatggc
       61 gcaaaataat tgatgaacaa tcgtagcgtt gtcactgcat cctccttgaa taccgcttga
      121 atgttgcgat ttgatggcag acgcatttaa cgcctgctga tggattgcac tatcatcaag
      181 atcacatcgg ccgagcacac ttcatcaaga aaacgacagt gtgcctttcc gcagattaaa
      241 ctgcagcttc aaaaagtcca gcgctttttc gccgcaccct gtcgcagatc aatgcgctgg
      301 cacagtatcg gcctcgtgcg tctccggtgc cggcccaagg ctcagaagcg ctactgcact
      361 caccgccatc agcacgatgg ccagcatcag gaagggggca tagctgccgg agaaatcgaa
      421 cacgtaaccc gcggtcaatg gcccaaacgc agtgcccatt gacagcgcca tcaccagcgc
      481 gccgtacagc gcgccgaaat tcttcaagcc gaaatgtttg gcagcgagaa aggcgatgac
      541 atccacctcc gatccgacag tgagaccgag cgcagcggca gcgattgact ggctgacggg
      601 atcactgccg gcaaacaaca gcagtaccgc cgaaacgatg ggcagcatga aggctgccgc
      661 accaaccaga tgaccgggaa atttgtccag cagcaaacca gtgcccagtc tgccgattat
      721 ggaaaagatg ccaatcagcg aagcgacacc cgctgccgcc agtggtgtgg tgccgctgtc
      781 cgtcaggatc ggcacaaaat gcacaagggc gccgatcgct gtaaacgaga acaggccact
      841 ggccagcaac agcttgtaga gttttggcga gcgcaaccct tcggacagtg taagtccggc
      901 gagaattttc gctgcaccgg gcacaacagc gccctgtttg cgcagcacat cctgagcacc
      961 gcgaaagaaa agaaacaaca cgggaaatac catggccgcc cagattccgc ccatggcgct
     1021 gaacgccgtc cgccagccgt aagcgcctat cagccaggtt gccagcagcg gaaaaaccgt
     1081 tgcactcaga gatgcaccgg acaatgtgat ggcaaatgca atgccgcgcg atgattcgaa
     1141 gcggctggcc accgcactgg tccacacggt ggcctgcacc cacaacgtcg agaaggcgac
     1201 gacaacccac aacaatatcc agtttgcgat cccgcccgtc gtggtgccca gcaaggcgaa
     1261 ggcggacagc atcagcaaca ctcccaccag cccaacccgt cggggaccca cccgatcaac
     1321 gagcatgcca atgggaatgc agaaaatcgc actgatgagg gatgaaatac tgattcccag
     1381 cgacgcttcg gcacgcgacc agccgaaggc ctgctgcaag ggttcaataa acggaccgag
     1441 cgaataaacg tgaatgaccg atgtcgaata gccaagcgca gcagcgaagg gcagaaacca
     1501 gaagctgcgc cactccgcga gcgtgatttt tgaaatagcc atccagttcc cctcagactt
     1561 ttacaccggt aaaggtgtac gagcccataa agccggcttt catttttcct gccatctgcc
     1621 taccgtcgag cactgctttg aactcgcact tcagttttat cggcttggta gcatgattga
     1681 tccaggagat gttgttacct tcaagcttcg catcaacgat gggagcaacg gtgccatcgc
     1741 cgctctgtgt gcccgtcagg cagccgtccc tgaactgcag gttaagcagg gtttgcagag
     1801 gtcccgtcgg cgcctttatg gtgatcttcc agtcgccttc gatcggcgct gccggatttt
     1861 ccgttttcac agtcgatcgc gaagtggcca tggccggctc tgccgcacca accgttgcaa
     1921 cgggggccga tacgatcgcc ttcggccgaa agatcgatgt gacgggcggc agatcagcat
     1981 aggtgacaat gccggggcga gcagccacaa cgcttggaat cgcattcaca acaggcatcg
     2041 cggtcatgat gaagccggta gcaaccagtt cttccatggg cagactgaga tcggcgggca
     2101 gaacgtcggc gcgcaatttc agctgcggcg taccattcac ttcaatgaga tag
//
LOCUS       DASUEG010001039         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_298791, whole genome shotgun sequence.
ACCESSION   DASUEG010001039 DASUEG010000000
VERSION     DASUEG010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298791"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFX11_19515"
     CDS             complement(<1..466)
                     /locus_tag="VFX11_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4940872.1"
                     /translation="MDIDLARTFLEIMRSGSFVQAAERLHVTQTTVTARVRTLEESLD
                     CQLFVRSRTGTRLTPDGERFVAYATTLVQTWDRARADLSLPVGHKTRLCIGTETSLWN
                     PLLLQWLLWMKDFRPDIALRTEVAEADVLLEQVEKGTLDAVLVHRPGYYSGLN"
     gene            594..1157
                     /locus_tag="VFX11_19520"
     CDS             594..1157
                     /locus_tag="VFX11_19520"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEX4940873.1"
                     /translation="MKDSLQHESPHWALLDQLGAHRPAPVDDTTLAGCSSPPCQQTHT
                     DPAYHGFLADADVVARLNVLLEAERAGALVCAQTLKQLPAGELHDLLLRIQHDEVKSC
                     RGLLRSIHLLNGTPSTAIGDFHPKAMAIADLAERLHLLDRGQAWVVRQLDALLPQVMQ
                     PEVFAQLQTMRDDHRDNIEKLRHESPR"
     gene            1225..1494
                     /locus_tag="VFX11_19525"
     CDS             1225..1494
                     /locus_tag="VFX11_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane sensor/regulator PpyR"
                     /protein_id="HEX4940874.1"
                     /translation="MLSTIGDIFNSRPVMFMKLLQLSRDVMVGSLMVMVAGLIVGFGL
                     ESKVGLGVQVIGHALAMIAAVGLKLGYIMRLEALSVLNASRRQDA"
     gene            1756..2418
                     /locus_tag="VFX11_19530"
     CDS             1756..2418
                     /locus_tag="VFX11_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4940875.1"
                     /translation="MNFKAPDSLSEQIARHLGEKIITGELKAGERIQELRVAGELNVS
                     RGSVREAYLILERRHLIDILPRRGAVVSEMTPRHVRNVYEVNILLLTQLARRATPVWK
                     EEDLAAFLQLLDEMDGYLQRNQAIDFHNASFRFARMAYRFAENPYLEEILEDLQPLMR
                     RTYYYAIRTDKIEMEKSMKMFRDLMERIIARDSDGAVKTLELFGQHQCETVLAFLESA
                     QD"
BASE COUNT          520 a          696 c          752 g          520 t
ORIGIN      
        1 cgttcaggcc ggagtaataa ccggggcgat gtaccagcac ggcatccagc gtgccttttt
       61 ccacctgttc cagcagcacg tcggcttccg ccacttcggt gcgcagcgca atgtccgggc
      121 gaaaatcctt catccacaac agccattgca acagcagggg attccacaga ctggtctcgg
      181 tgccgatgca gaggcgggtc ttgtggccta ccggcaggct caggtctgcc cgggcgcggt
      241 cccaggtctg caccagcgtg gtggcgtagg caacaaagcg ctcgccgtcc ggtgtcagac
      301 gcgtgccggt gcgactgcgc acaaacagct gacaatccag ggattcctcc agcgtgcgca
      361 cccgcgcggt gacagtagtc tgggtcacat gcagacgttc tgcggcctgc acaaaactgc
      421 ctgagcgcat gatttccaga aacgttcggg caagatcaat atccatgctg ctttccagca
      481 aaaaaattca taggtccggt gaccagcaac gatcaatacg cagcgtagta tggagcaacg
      541 gtccgccgac gcaagtacgg tcactgatct ggaaaaaaaa gcgggagaac cccatgaaag
      601 acagcctgca acacgaatcg ccacactggg cgctgctgga tcaactgggt gcgcaccggc
      661 cggcgccggt ggatgacact acgctggccg gatgcagttc gccgccgtgc cagcagactc
      721 acaccgatcc ggcctaccat ggatttctgg ccgatgccga tgtggtggcg cgactcaatg
      781 tcctgctgga agccgagcgg gccggtgcgc tggtctgtgc gcagacgctg aaacagttgc
      841 ccgccggcga gttgcacgat ctgctgctgc ggattcagca cgacgaagtg aaatcctgtc
      901 gtggcctgtt gcgcagtatt catctgctca atggtacgcc ttccactgct atcggtgatt
      961 tccatcccaa agcgatggcg attgcggatc tggctgaacg tctgcatctg ctggatcgcg
     1021 gccaggcctg ggtggtgcgc cagctggacg cgttgctgcc acaggtgatg cagccggagg
     1081 tgttcgcgca actgcagacg atgcgcgacg atcaccggga caatatcgaa aaattgcggc
     1141 acgaatcccc gcgctgatat cccttcaccc gcagcaaaat cattgcagca atgccgcagg
     1201 atttttcatt atcaccagga ggtagtgctc agtaccatcg gcgacatatt caacagcagg
     1261 ccggtgatgt tcatgaaatt gttgcagctt tcccgcgatg tgatggtggg gtcattgatg
     1321 gtcatggtgg cgggcttgat cgtgggattt ggcctggaaa gcaaagtggg gctgggggtg
     1381 caggtgatcg ggcatgcact ggccatgatc gcggccgtgg gcctcaagct ggggtacatc
     1441 atgcggctgg aggccctgtc cgtcctgaat gcatcccgtc ggcaggacgc ctgatcgatc
     1501 cgcagtagcc gctccatacc cttctttctt ttcgcttttc ctcttcatct tgccgtaatc
     1561 gtgtatccaa ctgtttcctg tgttgcgttt ccgtttccgg cttgcgcgga tatgggacaa
     1621 aagtacatgg atcatttatt atgcgcgcct attggtaggc aagacggcgt gactggggcc
     1681 atctgccggc atcttgctgg taccttgcgt ctaccgtata agatctttaa ccacaatact
     1741 aaaagcagtt cgaccatgaa tttcaaagca cccgacagcc tttccgagca gattgcgcgc
     1801 catttgggcg aaaagatcat taccggcgaa ctcaaggccg gcgagcgtat ccaggaattg
     1861 cgggtggcgg gcgaactgaa tgtcagccgg ggatcggtgc gcgaggctta cctgatcctg
     1921 gagcggcgcc atctgatcga catcctgcca cggcgtggtg cggtagtgtc agagatgacg
     1981 ccccggcatg tgcgcaatgt atatgaagtg aatatcctgc tgctgaccca gctggcgcgc
     2041 cgggctactc cggtgtggaa ggaagaggat ctggcggcgt tcctgcagct gctggatgaa
     2101 atggatggct atctgcagcg caaccaggcc atcgattttc acaacgcttc gttccgtttc
     2161 gcccgcatgg cgtaccgctt tgccgaaaac ccctatctgg aagaaatcct cgaagacctg
     2221 cagccgctga tgcggcgcac ctactattac gctatccgca ctgacaagat cgaaatggag
     2281 aaatccatga aaatgttccg cgacctgatg gagcggatta ttgcgcggga ttctgacggt
     2341 gcggtaaaaa ccctggaact gttcggccag caccagtgtg aaacggtgct ggcgtttctg
     2401 gaaagcgcgc aggactgaca ggcggggacg cggggagtgg caggtcggca tcaagaacgt
     2461 cagcggccat ggatggccgc tgtcgagc
//
LOCUS       DASUEG010001040         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_1010596, whole genome shotgun sequence.
ACCESSION   DASUEG010001040 DASUEG010000000
VERSION     DASUEG010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010596"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..824
                     /locus_tag="VFX11_19535"
     CDS             24..824
                     /locus_tag="VFX11_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX4940876.1"
                     /translation="MNASCKPESTPFYVPQSNEVTLFEHAFRHQLPVLIKGPTGCGKT
                     RFVRHMAAKLGLPLFTVACHDDLTATDLVGRHLLQDGDTVWCDGPLTRAVRDGGICYL
                     DEVVEARKDTTVVLHPLSDDRRELPIERTGELLHAPKNFMLVVSYNPGYQNLFKGMKP
                     STRQRFVAMRFDFPKPQIEKQVVIAETGVSERIADQLVQVAQALRRLQDHDLEESVST
                     RLLVYCAALIHSGLPWQSACRAALAEPLSDDEETVNALMDVIVAIMDA"
     gene            840..1229
                     /locus_tag="VFX11_19540"
     CDS             840..1229
                     /locus_tag="VFX11_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940877.1"
                     /translation="MNDPNQHDASQRRRAGLAYILYLLNLSFLPGIGFLILLALWHSP
                     PSRPPFVNTHYQQAAFASIVAGVLLAMVSVVIVLAGGFNSPWTFVILILYFTLCHSLL
                     LMLGVFGFTRANNGKPFQFFSPKSWRS"
     gene            1233..>2271
                     /locus_tag="VFX11_19545"
     CDS             1233..>2271
                     /locus_tag="VFX11_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940878.1"
                     /translation="MEEAVGKLWHKWITQASGSEFPDARVALPDVQKKIALWFHSFGG
                     APVLTIKAASREQWQARRSWLARIAGVEWQVQLCWKDNESLFLPGALTCFPDRALNEK
                     LYLWLAALAALPADTHASGHWIQRSQQQVQQALALWPGLRKTYQMLVDAHLQLRPLPT
                     SKNLQAQEQQLRDALRNPDQVVTTDIDPRTLAPVPLWLHPLPPATPSAQSSAPPPDIT
                     AETDQVTEIKSDHRRQGKQVDHSREDEGLLAFRLESLFSWAEFVNVDRPQEDDEELGA
                     KQAANDLDEFAVSRKSGTVKQRLKFDLDLPASDYDDVSTHEGIKLPEWDYRKNLLLKD
                     HCTLSLLYGRDI"
BASE COUNT          456 a          715 c          676 g          424 t
ORIGIN      
        1 gtgcccctta ccggatgaca agcatgaacg catcctgcaa gccagaatca acgcccttct
       61 acgttcccca gtccaatgaa gtcaccttgt tcgaacatgc attccggcat cagttgccag
      121 tgctgatcaa gggccccacc ggctgcggca aaacccgttt tgtgcgccat atggcggcga
      181 aactgggcct acccctgttc acggtggcct gtcacgacga cctcaccgcc accgatctgg
      241 tcgggcggca tttattgcag gatggcgata ccgtctggtg cgacggaccg ctcacccgcg
      301 ccgtgcgtga cggcggtatc tgctatctgg atgaagtagt ggaagcgcgc aaggacacca
      361 ccgtggtgct gcacccgctg tcggacgatc gccgcgaatt gcccatcgaa cgcaccggcg
      421 aactgctgca cgcgccgaaa aatttcatgc tggtggtgtc ctacaacccc ggctaccaga
      481 atctgttcaa gggcatgaag ccctccactc gccagcgctt tgttgcgatg cggttcgatt
      541 ttcccaaacc gcagatcgag aagcaggtgg tgatcgcaga aaccggtgtc agcgaacgca
      601 ttgccgatca actggtgcag gtggcacagg cgctgcgccg gctgcaggat cacgatctgg
      661 aggaatccgt atccacccgc ctgctggtgt actgcgccgc gctgattcac agcggactgc
      721 cctggcagag cgcctgccgc gccgcattgg cggagccgtt gtccgacgac gaggaaacgg
      781 tgaacgcgtt gatggatgtg atcgtcgcca tcatggatgc ctgaacggag aagcctgcca
      841 tgaacgaccc gaaccagcac gatgccagcc agcgccgccg cgccgggctc gcctacatat
      901 tgtatctgct gaatctgtcg tttctgccgg gcatcggctt tctgatcctg ctggcactgt
      961 ggcattcacc gccatcgcgc ccgcccttcg tgaacaccca ttatcagcag gctgcgttcg
     1021 ccagcattgt cgccggcgtg ctgctggcga tggtcagcgt cgtcatcgtg ctggccggcg
     1081 gtttcaacag cccctggacc ttcgtcattc tcatcctcta tttcaccctg tgccattcat
     1141 tgctgctgat gctgggcgtg ttcggcttca cccgcgccaa caacggcaaa cccttccagt
     1201 tcttcagccc gaaatcctgg cggagttgaa ccatggaaga agccgtcggc aaactgtggc
     1261 acaagtggat cacccaggcg tccggctcgg aatttcccga cgcgcgcgtg gccctgccgg
     1321 acgtgcagaa aaaaatcgca ctctggtttc acagtttcgg cggcgcgccg gtgctcacca
     1381 tcaaggcggc gtcgcgggaa cagtggcagg cacggcgcag ctggctggcc cgcatcgccg
     1441 gcgtggaatg gcaggtgcaa ctgtgctgga aggacaacga aagcctgttt ctgccgggcg
     1501 cgctgacctg ctttcctgat cgcgcgctga atgaaaaact ctatttgtgg ctggcagcgc
     1561 tggcagcgct accggcggac acgcacgcgt cgggacactg gattcagcgt tcgcaacagc
     1621 aggtgcagca ggcactggcg ctatggccgg ggctgcgcaa aacctatcag atgctggtgg
     1681 acgcacatct gcaattgcgt ccgctgccca catcaaaaaa cctgcaggcg caggaacagc
     1741 aactgcggga cgcgctgcgc aatccggacc aggttgtgac caccgacatc gatccgcgca
     1801 cactggcgcc ggtaccgctg tggctgcatc cgctgccgcc cgcaacacct tccgcacaat
     1861 cctcggcacc accgccggat atcacggcgg aaaccgatca ggtcaccgaa ataaaaagcg
     1921 atcaccgccg tcagggcaaa caggtggacc attcccgcga agacgaaggg ctgctcgcct
     1981 tccgcctgga aagtctgttt tcctgggccg agttcgtgaa tgtggaccgc ccgcaggaag
     2041 acgacgagga actgggcgcc aagcaggccg ccaacgatct ggatgaattc gcggtgtcgc
     2101 gcaagagcgg cacggtgaag cagcgcctga agttcgacct ggatctgccg gcatcggact
     2161 atgacgatgt cagcacgcac gagggcatca agctgccgga gtgggattac cgcaagaatc
     2221 tgttgttgaa ggatcactgc acgctgagtc tattgtacgg ccgcgacatc g
//
LOCUS       DASUEG010001041         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_531951, whole genome shotgun sequence.
ACCESSION   DASUEG010001041 DASUEG010000000
VERSION     DASUEG010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531951"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1712
                     /locus_tag="VFX11_19550"
     CDS             105..1712
                     /locus_tag="VFX11_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX4940879.1"
                     /translation="MFVFSDQERYFHKWPAGLSLPGHERLQKTGVTFHNHYCPAVMCT
                     SSRSVMLTGLQTATNGMFENTDVAYVPNLSTDFPTIGHMLRKAGYYTAYKGKWHLNRE
                     FEREEGDNTLTQKMDRYGFSDFYSPGDMIAHTLGGYEFDHLIAGSAITWLRRHGRRLT
                     DEGKPWCLFVSLINPHDIMYFNTDAPGINVQDTGSLTMHAARAPASRLYEASWDMPIA
                     KNLQQPFNDPGRPGAHGEFDKMWGYVLGRIPAEEERWRRFNDFYINSIRSVDLQVNSI
                     LGELDALRLADRTAFIYTSDHGEMAGAHGLRGKGPFAYQEAMHLPMYVVHPDVKGGQD
                     TRALTGHIDFAPTMLALAGVDATRAAEFAGRELPGKDFSPVLSNPGASKVHSTRESVL
                     FTYSGLVTNDGDLFQHVVEARSEGKKPILAMLKDGFKPDMKKRGSLRTVFDGRYKFTR
                     YFSPLDHNKPTTLDELYQFNDVELFDLASDPHEMVNLGADRNTNRDLLVAMNAKLNNV
                     IKAEIGVDDGRELPDIPMVDWTVGKIS"
     gene            complement(1805..>3820)
                     /locus_tag="VFX11_19555"
     CDS             complement(1805..>3820)
                     /locus_tag="VFX11_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX4940880.1"
                     /translation="QPLWKTIMTSPTRLVLLCCVLLLTACGEPQPPDTKPFQDVDADA
                     NGHTPATRVTTSANKAVEKNLTKDESDLEAARRGFIASDDPLDIRRADGKPIWTATDY
                     AFLDGKRPASVHPALWRQEQLNNLHGLFKVADGVHQVRGYDLANMTIIDGRRGRIIVD
                     PLTSVETARAAMALVEKNLGPRPLIAIIFTHSHVDHFGGVNALLESIKPGAAAPRIIA
                     PQGFMEEAVSENVLAGTVMMRRARYMYGSSLARSERGHVGSGLGKQPALGTISIARPT
                     DLITQTGQKLSLDGVRFVFQLVPESEAPAEMTFHLPEKKIWCGAELVSRTLHNLYTLR
                     GAKVRDALKWSGYIQQAIDLFAADTDTIINSHHWPVWGNDNARRYLAQQRDLYKFIHD
                     QTLRLASQGLTPPEIAEQLKLPASLTDSFANHGFYGTLKHNARAVYQFYFGWYDGNPA
                     HLNPLPPNQTAGRYVEAMGGAKAVKGRARTAYDDGDYRWAAELLNHVVFADPDDAQAR
                     ELLARSYDQLGYQAESAPWRDNYLTGALELRKGVVPPVLVANSSAMLQHIPMEQFFNA
                     IATLLNGDKAAGKQAVINLNFSDTREQYVLWLENAVLHHRQGSAEGADFSLTLTRTLW
                     QQLLLRQAGLSDLLLNDDFKLDGDRLKLLDFLSLLDQPQPDFAIVEP"
BASE COUNT          749 a         1059 c         1181 g          831 t
ORIGIN      
        1 ctgcggcagt agcgggcagc caattgcccg gaacggtatt tgcggatact ggaaagacag
       61 cacctgccca aatttctgca cgtacatcgt ccaggcccaa tctgattttt gtgttctcgg
      121 atcaggagcg ttatttccat aaatggccag cgggcctgtc attgcctggg cacgagcgtt
      181 tgcaaaaaac cggcgtgacc tttcacaacc actattgccc agctgtcatg tgcacctcat
      241 cacgttcggt catgctgaca ggtctgcaaa ccgctaccaa tggcatgttc gagaacaccg
      301 atgtggctta tgttccgaat ctttcaacag acttccccac cattgggcac atgttgcgaa
      361 aggccggtta ttacaccgcc tataaaggta aatggcacct gaaccgggaa ttcgaacgcg
      421 aggaaggtga caacacgctc acgcagaaga tggatcgata tggattttcg gatttttatt
      481 cgcccggtga catgattgcc cacacgctgg gcggatatga gtttgatcac ctgattgcag
      541 gcagtgcgat tacctggctg cgccgtcacg gcagacgcct gacggatgaa ggcaaaccct
      601 ggtgtctgtt cgtgagtctg atcaatccgc acgacatcat gtatttcaat accgatgcgc
      661 ctggcatcaa tgtgcaggac acgggctcac tgaccatgca tgcggcccgg gcgccagcca
      721 gcaggctgta cgaagcctcc tgggacatgc ccattgcaaa aaatctgcag caacccttca
      781 atgatcccgg acgcccgggt gcccatggtg aattcgacaa aatgtggggt tacgtgctcg
      841 gccgtattcc ggccgaagaa gaacggtggc gccgcttcaa tgatttctat atcaactcca
      901 tccgttcggt ggatctgcaa gtcaatagca ttctgggtga actcgatgcg ttgcggcttg
      961 cagaccgcac ggcgtttatc tacacctccg accacggtga aatggcgggt gcgcacggtt
     1021 tgcgtgggaa aggtccgttt gcctaccagg aagccatgca tctgccgatg tatgtcgtac
     1081 atccggatgt aaagggtggg caggataccc gcgctttgac ggggcatatc gactttgcac
     1141 ccaccatgct ggcgctggcc ggcgttgatg ccacccgcgc ggctgagttc gcaggacgtg
     1201 aattgccggg caaggatttt tcacctgtgc tgagcaatcc gggtgcttcc aaggtgcatt
     1261 caacccggga gtcggtgttg tttacataca gtggactggt taccaacgat ggcgatttgt
     1321 tccaacacgt tgtcgaagcc aggtctgagg gcaaaaagcc cattctggcg atgctgaaag
     1381 atggtttcaa acccgatatg aagaagcgtg gcagcctgcg gacggtgttt gacgggcgct
     1441 acaagttcac ccggtatttt tcaccgctgg atcacaacaa acccactaca ctcgacgagc
     1501 tgtatcagtt caacgatgtg gagctgtttg atctggccag tgatcctcac gaaatggtca
     1561 atctgggtgc cgatcgaaac accaacaggg atctgcttgt tgcgatgaac gcaaaactga
     1621 acaatgtcat caaggcagag atcggtgtgg atgatggcag ggagttgccg gatatcccca
     1681 tggtggattg gactgtcggt aagatcagct gatttgtgag gcagacgcga caacgttttc
     1741 tgacattgtt tgctggcgtt tgtgggcatc cggccttgtg agtagtccgg caaaagaccc
     1801 ggcctcaagg ttccacgatt gcaaagtctg gctgcggctg atccagcagg ctgagaaaat
     1861 ccagcagttt caggcggtcg ccgtccagtt tgaaatcgtc gttcagcaac aggtctgaca
     1921 agcccgcctg acgcagcagc agttgttgcc acagggtgcg ggtgagcgtc aggctgaaat
     1981 ctgcgccttc tgcactgccc tgccggtggt gcagcacggc gttctccagc cagagcacat
     2041 actgttccct ggtgtcgctg aaattcaggt tgatcaccgc ctgcttgccc gcggccttgt
     2101 cgccgttgag cagggtggcg atggcgttga agaactgctc catgggaatg tgctgcagca
     2161 tggcgctgga gttggcaacc agtacggggg gaaccacacc tttgcgcagt tccagcgcgc
     2221 cggtcaggta gttgtcgcgc cagggcgctg actccgcctg atagcccagc tggtcgtaac
     2281 tgcgggccag cagttcccgc gcctgcgcgt catccggatc ggcaaagacc acatggttga
     2341 gcaactctgc cgcccagcgg taatcaccat cgtcataggc ggtgcgggca cggcccttca
     2401 ccgccttcgc gccgcccatg gcttccacgt agcgtccggc cgtctggttg ggaggcaacg
     2461 ggttcaggtg ggcagggttg ccgtcgtacc agccgaaata gaattgatat acggcgcggg
     2521 cgttgtgctt cagggtgccg tagaaaccgt ggttggcaaa actgtcagtg agcgaggcgg
     2581 gcagtttcag ctgctcggca atctctggcg gggtgaggcc ctggctggcc agacgcagcg
     2641 tctggtcgtg aatgaatttg tacaggtcgc gctgctgtgc cagataacgc cgggcgttgt
     2701 cgttgcccca caccggccag tggtggctgt tgatgatggt gtcggtgtcg gcggcgaaca
     2761 ggtcgatggc ctgctggatg tagccggacc atttcaaggc atcccgcacc ttggcgccgc
     2821 gcagggtgta caggttgtgc agcgtgcgcg acaccagctc ggcgccacac cagatttttt
     2881 tctccggcag atggaacgtc atctccgccg gcgcttcgga ttccggcacc agctggaaca
     2941 caaagcgcac gccatcgagg ctgagtttct ggccggtttg cgtgatgagg tcagtgggcc
     3001 gcgcgatgga aatcgtgccc agcgccggtt gcttgcccag ccccgatccc acgtgccctc
     3061 gttcggagcg cgccagtgat gatccgtaca tgtaacgggc gcgccgcatc atcacggttc
     3121 cggccagcac gttttcgctc acggcttctt ccatgaaacc ttgcggcgca atgatgcgcg
     3181 gtgctgccgc gccgggcttg atcgactcca gcagcgcgtt cacgccgccg aaatgatcca
     3241 cgtggctgtg ggtgaaaatg atggcgatca gcggacgcgg acccagattt ttttccacca
     3301 gcgccatcgc cgcgcgggcg gtttccaccg acgtcagcgg atcgacaatg atgcgtccgc
     3361 gccggccgtc aatgatggtc atgttggcca ggtcatagcc gcgcacctgg tgcacgccgt
     3421 cggccacctt gaacaggcca tgcagattgt tgagctgttc ctgccgccac agggcgggat
     3481 gcacactggc agggcgcttg ccatcgagga aggcgtagtc ggtggcggtc cagattggct
     3541 tgccatcggc acggcggatg tcgaggggat cgtcgctggc gatgaaaccg cgccgggcgg
     3601 cttccagatc cgattcgtcc ttggtcaggt ttttttccac cgccttgttg gctgacgtgg
     3661 tgacgcgggt ggcgggggta tggccgttgg cgtcggcgtc cacgtcctgg aatggtttgg
     3721 tgtccggtgg ttgcggttcg ccacaggctg tcagcaggag cacacagcac agcagaacaa
     3781 gacgggtggg gctggtcatg atggttttcc acagtggttg
//
LOCUS       DASUEG010001042         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2026787, whole genome shotgun sequence.
ACCESSION   DASUEG010001042 DASUEG010000000
VERSION     DASUEG010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026787"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..606
                     /gene="gstA"
                     /locus_tag="VFX11_19560"
     CDS             7..606
                     /gene="gstA"
                     /locus_tag="VFX11_19560"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX4940881.1"
                     /translation="MKLYYSPGACSLSPHIVALEADLPLQLEKTDLAKKTTASGEDFL
                     KVNAKGYVPALKLDDGSVLTEGPAIVQYLADKAPASKLIPAAGTLERYRVLEWLNFIS
                     TELHKTFSPLFNPTTTDARKQEVREYLAKRFTTVEQQLAKTPFITGDSFTVADAYLFT
                     VLGWGKYVNVELPKPLQDYLGRVFQRPAVQQALKEEGLI"
     gene            798..>2272
                     /locus_tag="VFX11_19565"
     CDS             798..>2272
                     /locus_tag="VFX11_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX4940882.1"
                     /translation="MSAKHATPTVVSISLGSSRDDYDLSTRFLDQDLHVVRLGTDGDI
                     AQAEALVTQWQNKAAAIALEGLRDRQRVGAFQLQQRLDQRLRHRARHRPVTSGTELRD
                     ILQEWAIRHLQNEQKGFFNNANVLVFSGAQHYRSARVLAEFTDNLRFADPVLQLGIPK
                     FLNSLQALELYTTGSHQVLRWAPPQVLSPSLAPVQEWNHFILRKALHKADVVIAHFDQ
                     LDAFGLEELAGKTLITSAISEERLKSLRDRGVYTVIDYTPQPFQHTVGIAVLQAMMLA
                     ALEKKNSDLTEDDYLDIITRLELQPRVLYPRGFKRLSRFAFVIHPLSQQYFRNAKPID
                     LVANIAPKPVMNTLEKIIAYSPPFVYSRVSGIQSPTGAEAEGWLITVGGTPKEIMRHG
                     PEFTYRRLLAAADMAKKLGAQIMGLGAFTKVVGDAGVTVAKRAPLPITTGNSYSASGA
                     LWAAHDAVKKLGLVQVGPSGKMAGKAMVVGATGAIGSVCARL"
BASE COUNT          471 a          759 c          653 g          389 t
ORIGIN      
        1 gatgttatga aactgtacta ctcaccgggc gcctgctccc tgtcccctca tatcgtcgca
       61 ctggaagcgg atctgccgct gcaactggaa aaaaccgatc tcgccaagaa gaccaccgcc
      121 tcgggcgagg acttcctgaa agtgaatgcc aagggctacg tgccggccct gaaactggac
      181 gacggcagcg tgctcaccga aggccccgcc atcgtgcagt atctggcgga caaggcgccg
      241 gccagcaagc tgatccccgc cgcaggcacc ctggaacgct accgcgtgct ggagtggctg
      301 aacttcattt ccaccgaact gcacaaaacc ttctcgccgc tgttcaaccc caccaccacc
      361 gacgcgcgca agcaggaagt gcgtgagtac ctggccaagc gcttcactac cgtcgagcag
      421 cagctggcaa aaaccccgtt catcacaggc gacagcttca ccgtggcaga cgcctacctg
      481 ttcaccgtgc tgggctgggg caaatacgtg aacgtggaac tgcccaagcc gctgcaggac
      541 tacctgggcc gcgtattcca gcgtcccgcc gtgcagcagg cactgaagga agaaggcctg
      601 atctgatccg gttttctgct gcaaccgcgc ctccaggcac caccgcccgg aggcgttcct
      661 tcaacgcatt cgctttaatt tcaccgccgt tcagcgcatt ttcaccggca tttacaaacc
      721 cttgatctgg gctaacgtcc gcgcgccacc tttctgctag aaatgtttac tcacgtcagg
      781 aaggactgga cccgctcatg agcgcaaaac acgccacccc caccgtcgtc agcatcagcc
      841 tgggatcgag ccgcgacgac tacgacctct ccacccgctt tctggaccag gatctgcacg
      901 tggtgcggct gggcacggac ggcgacatcg cccaggccga agcactggta acacagtggc
      961 agaacaaggc cgccgccatc gcgctggaag gcctgcgcga tcgccagcgg gtgggtgcct
     1021 tccagttaca gcagcgactg gaccagcgtc tgcgccaccg cgcccgtcat cgccccgtga
     1081 ccagcggcac ggaactgcgc gacatcctgc aggaatgggc catccgtcat ctgcagaacg
     1141 aacagaaagg ttttttcaac aacgccaacg tactggtgtt cagcggcgcg caacattacc
     1201 gctccgcccg cgtactggcg gaattcaccg acaacctgcg cttcgccgat ccggtgctgc
     1261 agctgggcat cccgaagttc ctcaactcac tgcaggcgct ggagctctac accaccggca
     1321 gccatcaggt gctgcgctgg gcgccgccgc aggtgctgtc gccgtcgctg gcgccggtgc
     1381 aggaatggaa tcacttcatc ctgcgcaagg cgttgcacaa ggctgacgtg gtgatcgccc
     1441 acttcgatca gctcgatgca ttcgggctgg aagaactggc gggcaaaacc ctgatcactt
     1501 ccgccatttc ggaagagcgt ctgaaaagcc tgcgcgaccg cggcgtgtac acggtgatcg
     1561 actacacgcc gcaaccgttc cagcacaccg tcggtattgc ggtactgcag gccatgatgc
     1621 tggccgcgct ggagaagaaa aacagcgatc taaccgaaga cgactacctc gacatcatca
     1681 cccgcctgga actgcaaccg cgtgtgctgt atccgcgcgg tttcaaacgc ctgagccggt
     1741 ttgccttcgt catccatccg ctgtcgcagc agtatttccg caacgccaag cccatcgatc
     1801 tggtggcgaa tatcgcaccc aagccggtga tgaacacgct ggaaaaaatc atcgcctact
     1861 ccccaccgtt cgtgtattcg cgtgtcagcg gcattcaatc ccccaccggc gcggaagccg
     1921 aaggctggct cattaccgtg ggcggcacgc cgaaggaaat catgcgacac ggcccggagt
     1981 tcacctaccg ccgtctgctg gcggcggcgg acatggcgaa aaaactgggc gcacagatca
     2041 tggggctggg cgccttcacc aaagtggtgg gcgacgccgg cgtcacggtc gccaagcgcg
     2101 ccccgctgcc gatcaccacc ggcaacagct acagcgcgtc cggtgcattg tgggcggcgc
     2161 atgatgcggt gaaaaaactc ggcctggtgc aggtcggccc cagcgggaag atggctggca
     2221 aggcaatggt ggtgggcgca accggggcca tcggttccgt ctgcgcacga ct
//
LOCUS       DASUEG010001043         5558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_237299, whole genome shotgun sequence.
ACCESSION   DASUEG010001043 DASUEG010000000
VERSION     DASUEG010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237299"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFX11_19570"
     CDS             complement(<1..1062)
                     /locus_tag="VFX11_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEX4940883.1"
                     /translation="MLKVLACVLLATAVISAQAAETFSFQTVIDKARDLAAQPYHEPE
                     PIPRFMRELTYDQYQDIRFNPDKSLWKQGKSKFQVMLMPPGLFYGHAVKVNIVDGQGA
                     KPLPFKKDLFTFTDPELEKLVPADLGYAGLKLTFPFFQPDIQNQFLVFAGASYFRGVG
                     KDNGWGISTRGIAIDTGLPTGEEFPSFLEFWLVHPAANSDQMMVYGLLDGPSLAGAYQ
                     FNIKPGKRTSVDVKAVLYPRNNIKLMGVAPLTSMFYYGENTSRPRGEWRSQVHDSDGL
                     LIHNGTGEWLWRPLINPVYLDMNYFRADDLNGFGLIQRDNGFNDYHDLGARYEIRPST
                     WVKPEGKWGPGYVVLVQLPT"
     gene            complement(1314..2537)
                     /locus_tag="VFX11_19575"
     CDS             complement(1314..2537)
                     /locus_tag="VFX11_19575"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX4940884.1"
                     /translation="MDIAELKKFEFLKHLSDQQLIMLVNNSESLKLKAGEPLIDAGDT
                     DGWEYFLLAGELDLIPPEGQPVRTLQANSPEALKALVRQRPRPVNVVAHSAAAVLKVD
                     LENLKNLLREAPSDSYEVRKALREDQPEDKQLLLDIYADLRNNKLILPSLPEVAVRIR
                     RLIDEGTNSAKKISQAVNTDPVIAAKLIKAANSPLFRGTKEFETSSQAIVRLGMQTTK
                     QLVTSFTLKELFKARSNVLKKRMDALWQHSIEIAAICFVLAKHARGVDPEQGLLAGLL
                     HDIGTVPILMYAEQYDTLVNDSQLLEKTIKELKTELGGVILKRWSFSEEMINATQHSE
                     NWRYQHEGGADITDLVIVAHLHQLMGSAEKAAQLDKVPAFRRLFPGELPFTQLTAILE
                     EAKIQLDETRQLLVA"
     gene            2836..4962
                     /locus_tag="VFX11_19580"
     CDS             2836..4962
                     /locus_tag="VFX11_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4940885.1"
                     /translation="MYGFPEFKNLVDMASQQAAERRDATVATFITDNGHIDSRMSFSN
                     LDEDARRIALVLVEKGLAGSNLLLLYTPGLDYIRAFFGCLYAGCVPVPAYPPVGAKDL
                     VRLQKVALDCNAGAILSNSTLAPLIEAWVTNLGQGVEIPCIATDKLNASANISGFVPH
                     QAGPDDVAFLQYTSGSTGHPKGVMVTHGNLLANFKQILCGFFQGNEMVDALADLRIAI
                     WLPPFHDMGLIGGVLTPIFAGAHVSLMSPLTFLKRPLLWLQTISEQKAHVSGGPNFGY
                     QYCARKVTEEQAETLDLSSWQVAFNGAEPIQLEALNSFSERFRVSGFNPKAFLPCYGL
                     AEASLFVAGSPSGRGARVLKAQLDQMEKGRFAAAESGYQGKCAELVSSGVLAVGTDVK
                     IVNPQTKRPCADGEVGEIWVQGPSVAKGYWEKPQFSAGVFQAVMAGDASGTEYMRTGD
                     LGFLWHAELFVTGRIKEVIIIAGRNLYPQDIELTLQEANPAFKLGGGAAFSVTHDGKE
                     QLVILQEVSRAAGDQADYKLLAAEGARAVASRYGVMPMALVLVAGSSIPKTSSGKLQR
                     SEARQMYKEGNFAPLYTWQPGGGNQRRAAIASHSHLDDLNVDWQSELYADVQGWVAEK
                     LDVEPHHIDLDVTFSELGVDSVEAVELVDRLQDRIERTIPAIELLRYPTVKALIDHFS
                     AELSQRALDKVLREEDGEVVEQVLEE"
     gene            complement(5029..>5558)
                     /locus_tag="VFX11_19585"
     CDS             complement(5029..>5558)
                     /locus_tag="VFX11_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX4940886.1"
                     /translation="MQTDPNFGNYFVRLGNGSDIPDRIVLLDFGAVREFSSHTIDPGR
                     TLVKAAFLHDEALLFQSLEALKMVAPDTPDNAKRSLSKLCFMAIEPFADPDRFPPPPE
                     LLTAKGEYRWGASNLPSRLSVQAGMSAAGSSRHFTVPPRELMFLVRKIMGAYTFMSVL
                     NAEIRGHEILRPYLK"
BASE COUNT         1035 a         1546 c         1786 g         1191 t
ORIGIN      
        1 ggtgggcagc tgtaccagca cgacataacc aggaccccac ttgccttcgg gcttgaccca
       61 ggtgctgggg cggatttcat agcgcgcgcc cagatcgtgg taatcgttga aaccgttgtc
      121 gcgctggatc aggccgaaac cgttcaggtc atcagcccgg aagtaattca tgtccaggta
      181 gaccgggttg atcagcgggc gccacagcca ctcgccagtg ccgttatgaa tcagcaggcc
      241 gtcggagtca tgtacctggc tgcgccattc gccacggggg cgggaggtgt tttcgccgta
      301 gtagaacatg ctggtcagcg gtgcaacgcc catcagcttg atgttgttgc gcgggtagag
      361 cacagccttc acgtccaccg aggtacgctt gccgggcttg atgttgaact ggtaggcgcc
      421 tgccaggctg gggccatcca gcaggccgta taccatcatc tggtcggaat tggcggccgg
      481 gtgcaccagc cagaactcca ggaacgaggg gaattcctcg ccggtgggca ggccggtgtc
      541 gatggcaatg ccccgggtgg aaatgcccca gccgttatcc ttgcccacgc cgcggaagta
      601 gctggcgccg gcaaacacca ggaactggtt ctggatgtcg ggctggaaga acgggaaggt
      661 cagcttcagg ccggcgtagc ccaggtcggc aggcaccagc ttctccagtt cggggtctgt
      721 aaaggtgaac aggtccttct tgaagggcag gggtttggca ccctggccat cgacgatatt
      781 gaccttcacc gcgtggccat agaacaggcc cggcggcatc aacattacct ggaatttgga
      841 tttgccctgc ttccacagac tcttgtccgg gttgaagcgg atgtcctggt actggtcgta
      901 ggtgagttcc cgcatgaagc gcgggatcgg ctccggctca tggtacggtt gggcagccag
      961 atcgcgagcc ttgtcgataa ctgtctgaaa gctaaatgtt tcagccgcct gagcggagat
     1021 taccgccgtt gccagcaata cacatgcgag aactttcaac atgaatccat gactccattg
     1081 tccgggttca aaacattgat tgggcattga ccgatacccc cgcgacgttc cgcacgacag
     1141 accgcattct gcaaggaatg tgttcgagac aaagccagga aatcattgcg ttttcacgtc
     1201 gggcgatcgt gatgatgccg ctggggcgtt attgcgccgg cattatcgca caggaaattt
     1261 cgggaacaaa tgcgcagggt tgtaaatatt ttatgaacag tccctgcgcg ggctcaggcc
     1321 accagcagct gccgggtttc atccagctgg atttttgctt cctccagaat cgccgtcagc
     1381 tgggtgaagg gcagttcgcc tggaaaaagg cggcggaaag cgggcacttt gtccagctgg
     1441 gccgcttttt ccgcgctgcc catcagttgg tgcaggtggg cgacgatgac caggtcagtg
     1501 atgtcggcac cgccctcatg ctggtaccgc cagttttccg agtgctgggt ggcattgatc
     1561 atttcctcgc tgaagctcca gcgtttgagg atgacgccgc ccagttcggt tttcagttcc
     1621 ttgatggtct tttccagcaa ctgggaatcg ttcaccaggg tgtcgtattg ctcggcgtac
     1681 atgaggatgg ggactgtgcc gatgtcatgc agcaatccag ccagcaggcc ctgctccgga
     1741 tcgacgcccc gggcgtgctt ggccagcacg aagcagatgg cggcaatttc gatgctgtgc
     1801 tgccagaggg catccatgcg ttttttcagc acgttcgacc tggccttgaa cagctccttg
     1861 agggtgaaac tggtgaccag ctgcttggtg gtctgcatgc cgaggcggac gatggcctgg
     1921 gaactggttt caaattcctt ggtgccgcgg aacagggggc tgttggcggc cttgatcagc
     1981 ttggcagcga tcaccgggtc ggtattgacg gcctgggaaa tctttttcgc gctgttggtg
     2041 ccttcgtcga tcaggcgacg gatgcgcacg gccacttccg gcaacgacgg cagtatcagc
     2101 ttgttgttgc gcaggtcggc gtagatatcc agcagcagtt gtttgtcttc cggctggtcc
     2161 tcgcgcaggg ccttgcggac ctcgtaactg tcggacggcg cttcccgcaa caggttcttc
     2221 aggttttcca gatccacctt caatactgcc gccgccgaat gtgccaccac attcaccggg
     2281 cgtggccgct gccgcaccag ggctttcagc gcttccgggc tgttggcctg cagggtgcgc
     2341 accggctggc cttccggcgg gatcaggtcc agctccccgg cgagcaggaa gtattcccag
     2401 ccatcggtgt cgccggcgtc aatcagcggt tcccccgcct tgagtttgag ggattcgctg
     2461 ttgttcacca gcatgatcag ttgctggtcg ctgaggtgtt tcaggaactc aaatttcttc
     2521 agttcagcga tgtccatagt gattcttcag tctgctgtgc ggtgggcggc aaggtttgtt
     2581 tattaagtat aggaagcgat tggggaaagt aggaatttcg cgccgggaat gtgaggcagc
     2641 ccgcagcgga acgcggtttg cgaccgttgg tcgtgccggt gcggaactga caggtctcct
     2701 gacacaaacg gctacaaatc ctcacttgtt tagtccttgt gatggtccgc aggtgcttgt
     2761 ttaaatgggg tattggtttc ggtgttgtac cggccaaaaa aacaataata cgggccagct
     2821 gaaaccgagg tacctatgta cggatttccc gaattcaaga atttggtcga tatggcgtcg
     2881 cagcaggcgg cggaacggcg cgatgcgacg gttgccacgt tcatcaccga caatggccat
     2941 atcgacagcc gcatgtcgtt ttccaatctc gatgaggatg cccgccggat tgcgctggtg
     3001 ctggtggaaa aggggcttgc cggcagtaac ctgctgctgc tctacactcc gggtctggat
     3061 tatattcgcg ccttttttgg ctgcctttat gccggctgcg tgccggtgcc ggcttatcct
     3121 cccgtgggcg cgaaggatct ggtgcggctg cagaaagtcg cgctggactg caacgcaggc
     3181 gccattctgt cgaacagtac cctggcgccc ctgattgaag cctgggtgac gaatcttggc
     3241 cagggtgtcg aaataccctg catcgccacc gataagctga atgcatccgc caacatcagc
     3301 ggtttcgtgc cgcaccaggc cgggccggat gacgtcgctt tcctgcagta cacctccggt
     3361 tccaccgggc atcccaaggg cgtcatggtg acccatggca acctgctggc gaacttcaag
     3421 cagatcctgt gcggcttttt ccagggcaat gaaatggtgg acgcgctggc ggacctgcgc
     3481 attgccatct ggctgccgcc tttccacgac atggggttga tcgggggtgt gctgacgccg
     3541 atatttgcgg gtgcccatgt ctccctgatg tcgccgctca ccttcctcaa gcgccccttg
     3601 ctctggttgc agaccatttc cgagcagaag gcccacgtca gcggcggccc caacttcggt
     3661 taccagtatt gcgcccgcaa ggtaacggag gagcaggcgg aaacgctgga cctgtccagc
     3721 tggcaggtgg cgttcaatgg tgccgagccg atccagctgg aggcgctcaa cagtttctcc
     3781 gagcgtttcc gtgtcagcgg tttcaatccc aaagccttcc tgccctgtta cggtctggca
     3841 gaggcgagcc tgttcgtggc cggctcgcca tcggggcggg gcgcccgggt gctgaaggcc
     3901 cagctggacc agatggaaaa aggccgtttt gctgcggccg agtccggtta tcagggcaaa
     3961 tgtgccgagc tggtcagttc cggtgtgctg gcggtgggta ccgatgtgaa gattgtgaat
     4021 ccccagacca agcggccttg cgccgatggt gaagtgggcg agatctgggt gcagggcccg
     4081 tccgtggcga aaggttactg ggagaaaccg cagttctctg ccggcgtgtt ccaggccgtg
     4141 atggcgggcg atgccagtgg caccgaatac atgcgcaccg gcgaccttgg cttcctgtgg
     4201 catgcagagc tgttcgtcac tggccgcatc aaggaagtga tcatcattgc ggggcgtaac
     4261 ctgtatccgc aggacatcga gctgacgctg caggaggcca atccggcgtt caagctgggc
     4321 ggcggtgcgg ctttttccgt gacccacgac ggcaaggaac agctggtgat cctgcaggaa
     4381 gtcagccggg cggcggggga tcaggcggat tacaaactgc tggcggcgga aggtgcgcgg
     4441 gcggtggcat cgcggtacgg tgtgatgccg atggcactgg tgctggtggc gggcagctcc
     4501 attcccaaaa catccagcgg caagctgcag cgcagcgaag cccggcagat gtacaaggaa
     4561 ggcaattttg ccccgctgta tacctggcaa ccgggtggcg gcaatcagcg cagagcggca
     4621 atcgccagcc attcccatct ggacgatctg aacgtggact ggcaatccga actgtatgcc
     4681 gatgtgcagg gctgggtggc ggagaagctg gatgtcgagc cccaccatat cgacctggac
     4741 gtgacgttct ctgagcttgg agtggattcc gtcgaggcgg tggagctggt ggaccggctg
     4801 caggaccgca tcgaacgcac cattcccgcc atcgagctgc tgcgctaccc gacggtgaaa
     4861 gcactgatcg accatttttc ggcggaactg agccagcgtg ccctggacaa ggtgctgcgg
     4921 gaagaagatg gtgaggttgt cgagcaggtg ctggaggaat aagggttcag gtcacccggc
     4981 ggaagcagca gtgtctgctg tcccgtcggg actgtttcaa tcagatcttt atttcaggta
     5041 cggccgcaga atctcgtggc cgcgaatctc tgcgttcagc accgacatga aggtgtaggc
     5101 ccccatgatc ttgcgcacca ggaacatcag ttcccggggc ggtaccgtga agtggcggct
     5161 ggagccggcg gcagacattc ccgcctgcac gctcaggcgg gacggcaggt tactggcgcc
     5221 ccagcggtat tcgcccttcg cagtgagcag ttcgggcgga ggcgggaacc tgtccggatc
     5281 ggcgaagggc tcgatcgcca tgaagcagag tttgctcagg ctgcgcttgg cgttgtctgg
     5341 cgtgtccggt gccaccattt tcagcgcttc caggctctgg aacagcagcg cttcatcgtg
     5401 caggaaagcg gctttcacca gggtgcgacc gggatcgatg gtgtggctgg aaaattcccg
     5461 caccgcgccg aaatccagta gcacgatccg gtccggaatg tcggacccgt tgcccagacg
     5521 cacaaagtag ttgccgaaat tgggatcggt ctgcattt
//
LOCUS       DASUEG010001044         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Kapaibacterium sp. isolate SMAG_U1346
            k141_2203487, whole genome shotgun sequence.
ACCESSION   DASUEG010001044 DASUEG010000000
VERSION     DASUEG010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961499
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Kapaibacterium sp. (soil metagenome)
  ORGANISM  Candidatus Kapaibacterium sp.
            Bacteria; Pseudomonadati; Candidatus Kapaibacteriota; Candidatus
            Kapaibacteriia; Candidatus Kapaibacteriales; Candidatus
            Kapaibacteriaceae; Candidatus Kapaibacterium.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:07:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,921
            CDSs (total)                      :: 3,889
            Genes (coding)                    :: 3,866
            CDSs (with protein)               :: 3,866
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Kapaibacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203487"
                     /isolate="SMAG_U1346"
                     /isolation_source="soil"
                     /db_xref="taxon:2447898"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFX11_19590"
     CDS             <1..622
                     /locus_tag="VFX11_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4940887.1"
                     /translation="SIIPEFFMRFLIWILTHTMYRVKHTNLDRIPDEGPALLVCNHVS
                     FMDALIIAGACRRPVRFVMFKPIYDMPVLNFIFRTGKTIPIHSKNVDPVTYENAFTRI
                     SQELRDGEVVCIFPEGKLTTTGEIDEFKNGMEKILAQDPVTVVPMALRGLWGSFFSHK
                     DSSAMTRLPRRFWSKVELVAGDPVAPDQARAAMMREKVQALRGDWK"
     gene            complement(649..1455)
                     /locus_tag="VFX11_19595"
     CDS             complement(649..1455)
                     /locus_tag="VFX11_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4940888.1"
                     /translation="MEKTLPDFIADELIARIYVGQLKPGDRLPAERQLAETLGVDRTS
                     LRMALRTLNRMHLVRPVRGSGITVTDYRKHAGLDFLAAILDIPELELGTDIKLESVDA
                     FNSILSGLIYQMVRGTIDARLVEEVRDLLQLQLRLLEEKPLNELLLDQLAELDLRLQE
                     ILLYRRGGMIVELMSNSTRPLRATVIRELFGLIDVRAYVEFHQDLIMKIGTGVLPVDQ
                     VAGYYYQYTVQLMEPLKQRYRANPQSPRLRASPLKSALARQVDPVSSQGL"
     gene            complement(1548..1736)
                     /locus_tag="VFX11_19600"
     CDS             complement(1548..1736)
                     /locus_tag="VFX11_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4940889.1"
                     /translation="MKDEHKRRKLRNRLALDPLLQKGGAHGKTKKAERAAQKQQTRQL
                     AMNRGDDSSPFHFFCLST"
     gene            complement(1806..>2935)
                     /locus_tag="VFX11_19605"
     CDS             complement(1806..>2935)
                     /locus_tag="VFX11_19605"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX4940890.1"
                     /translation="QAYQQLANIASLPIVHGHIAAMPDVHLGKGATVGSVIPTRNAII
                     PAAVGVDIGCGMNAVRLSLKAWQLPDNLRQLRGEIEDRVPVGFNAHKWDNSRDHACKQ
                     LSGDLDRILGQHPAIGRMMKNIGKTWVCQMGSLGGGNHFIELCLDENQDVWVMLHSGS
                     RGIGNNIGQYFIGLARKDMGQHLHQLPDKDLAYFSEGNPHFDDYVFAVHWAQDYAMQN
                     RREMMKLVVEALRVHLPDFDLTAEAINCHHNYVAIEPHMGHKLYITRKGAIRAGAGEL
                     GIIPGSMGAKSYIVRGKGNPESFCSCAHGAGRRMSREQAKKRFQREDLERQTKGVECR
                     KDKGVIDEIPAAYKDIDEVMANQTDLVEIVHTLKQVLCVKG"
BASE COUNT          572 a          865 c          853 g          645 t
ORIGIN      
        1 ttccatcatt cccgaattct tcatgcgctt tcttatctgg atcctcaccc ataccatgta
       61 ccgggtgaag cataccaatc tggacagaat tcccgacgaa ggccctgccc tgctggtgtg
      121 caaccatgtg tcgttcatgg atgcactgat catcgccggt gcctgccgcc gcccggtgcg
      181 ctttgtgatg ttcaagccga tctacgacat gccggtgctg aatttcattt tccgcaccgg
      241 caagacgatt ccgatccact cgaaaaatgt cgacccggtg acctacgaaa atgcgttcac
      301 ccggatttcc caggaattgc gtgacggcga agtggtgtgc attttccccg aaggcaaact
      361 taccaccacc ggcgagatcg acgaattcaa gaacggcatg gagaaaatcc tggcacagga
      421 cccggtgacc gtggtgccca tggcgctgcg tggtctgtgg ggcagcttct tcagccacaa
      481 ggacagcagc gcaatgacac ggctgccacg ccggttctgg tcgaaggtgg aactggtggc
      541 gggcgatccg gtggcgccgg accaggccag ggcggcgatg atgcgcgaga aagtacaggc
      601 cctgcggggt gactggaaat aaaccggtaa tgatcgggcc ggtgccgttt acaggccctg
      661 gctggaaacc gggtccacct gccgcgccag cgccgacttc aagggtgaag cacgcaggcg
      721 cggtgactgg gggttggcac ggtaacgctg tttcagcggt tccatcagct gtaccgtgta
      781 ttgatagtaa taaccggcca cctgatccac cggcagcacg ccggtaccga ttttcatgat
      841 cagatcctgg tggaattcca cgtaagcccg cacatcgatc aggccgaaca gctcgcggat
      901 tacggtggcg cgcagcggac gggtggaatt cgacatcagt tcgacaatca tgcccccgcg
      961 ccggtacagc aggatttcct gcagccgcag atccagctcc gccagctggt ccagcagtag
     1021 ttcattgagc ggcttttctt ccagcagcct cagctgcagt tgcagcagat cacgcacctc
     1081 ctccaccagc cgggcgtcga tagtgccacg caccatctgg taaatcaggc cggacagaat
     1141 gctgttgaac gcgtccaccg attccagctt gatgtcagta cccagttcca gttcggggat
     1201 gtcgagaatg gcggcgagaa aatcgaggcc ggcgtgcttg cgataatcgg tgacagtgat
     1261 gccggaaccg cgcacgggcc ggaccagatg catgcgattg agggtgcgca gggccatgcg
     1321 cagggaagtg cgatcgacac cgagggtttc ggcaagctgg cgttctgccg gcagccggtc
     1381 gcccggcttg agctggccga catagatacg ggctatcagt tcatcggcaa tgaaatccgg
     1441 aagggtcttt tccattggcg gtgtcgtttc ttgttatcgg attttgttga agtctatcac
     1501 aaaaacaggg attcgccggg agcaccagaa aggggtagtg ggcaggatca ggttgacagg
     1561 cagaaaaaat gaaaaggcga tgaatcgtcg ccgcgattca tcgccagttg ccgggtttgc
     1621 tgcttttgcg ccgcccgttc ggcctttttg gttttgccgt gggcaccgcc tttctgcagc
     1681 agcgggtcca gggccagccg gttacgcagt ttcctgcgct tgtgttcgtc tttcatgggg
     1741 tttctcccgt ggttttttcc agtagtctct tcctgcagtc tcttcctgtg tggatacctt
     1801 gcctttcagc ctttcacgca cagcacctgt ttcagagtgt gcacgatttc caccagatcc
     1861 gtctggttgg ccatcacctc gtcgatatcc ttgtaggccg ccggaatctc gtcgatgacg
     1921 cccttgtcct tgcggcattc cacgcccttg gtctgccgtt caagatcctc gcgctggaag
     1981 cgctttttcg cctgctcccg gctcatcctg cgcccggcac cgtgagcgca ggaacagaag
     2041 gattccggat tgcccttgcc acgcacgatg tacgatttgg cgcccatgct gccggggatg
     2101 atgcccagct cgcccgcgcc agcgcgaatg gcgcccttgc gggtgatata gagcttgtgc
     2161 cccatatgcg gctcgattgc cacgtagttg tgatgacaat tgatcgcctc tgccgtcaga
     2221 tcgaaatccg gtaaatggac gcgcaacgcc tccaccacca gtttcatcat ttcgcgccgg
     2281 ttctgcatgg cataatcctg cgcccagtgc accgcaaata cgtaatcgtc gaaatgcgga
     2341 ttgccttcgc tgaaatacgc cagatccttg tcgggcagct ggtgcagatg ctggcccatg
     2401 tccttgcgtg ccaggccaat aaaatactgg ccgatgttgt tgccgatgcc acgactgccg
     2461 gaatgcagca tcacccacac atcctggttt tcatccagac acagttcgat gaagtggttg
     2521 ccgccaccga gcgagcccat ctggcacacc caggttttgc caatgttttt catcatgcga
     2581 ccaatggccg gatgctgccc cagaatgcgg tcgagatcgc cggacagctg tttgcaggcg
     2641 tgatcgcgac tgttgtccca tttgtgggcg ttgaaaccca ccggcacacg atcctcgatt
     2701 tcaccgcgca actggcgcag gttgtccggc agttgccagg ctttcagcga cagccgcact
     2761 gcattcatgc cgcaaccgat atccacaccc actgcggcgg gaatgatggc gttgcgggtg
     2821 ggaattactg atcccaccgt ggcgcccttg cccagatgca catcaggcat ggcggcaata
     2881 tgaccgtgca cgatgggcag gctggcgatg ttggccagct gctggtacgc ctgcg
//
